LOCUS       DASRTJ010000001         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75747,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000001 DASRTJ010000000
VERSION     DASRTJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75747"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFF70_00005"
     CDS             complement(<1..1197)
                     /locus_tag="VFF70_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY43106.1"
                     /translation="MSHTRKMWALIAVLMVVSMVISACQPAATPAPTAAPTTAPKPTT
                     PPPAPTAAPTAVPVTMLSKAAPDCTWGGTLKSIEAKDDTTVVFTMCTPDPAFPSKAAF
                     ASLAIQSAAHLTASGGKPLEDALGTGAYMVKEWVRGDHLTLVPNPNYWGDKPKTGTVI
                     FKWNKEAAARLTSLKSGEIDGMDNPDPNDFKAIEADATLKLYPRVALNVFYLGINNTK
                     PPFDNEKVRQAIGMGIDRQAIVDKYYPVGSSVADYFTPCAIPGGCEGDPWYKFDPVAA
                     KALLAGVTMPKGITLSYRDVVRGYLPTPGKVAEELQAQLKKNLGIDVTIDVQESGTFL
                     DNSSKGNLSLHLLGWGADYPDQTDFLDYHFGKGASAQFGTKFDDLTAVLAQAASLADQ
                     TARNKLY"
     gene            complement(1399..1767)
                     /locus_tag="VFF70_00010"
     CDS             complement(1399..1767)
                     /locus_tag="VFF70_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZY43107.1"
                     /translation="MKILQEATGSDRVVFRIVPSGRLDAVTVPTLEEFLQSELSAGHN
                     YLILDLSEVSYLSSSGLRALLQARRQAQAAGGDLVLAAMSVRAQEIFEMIGFTNLFRV
                     FDRVAEAVVALTQPAVNSKS"
     gene            complement(1808..2248)
                     /locus_tag="VFF70_00015"
     CDS             complement(1808..2248)
                     /locus_tag="VFF70_00015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43108.1"
                     /translation="MMVREERHLTVPGRFESLARISEFVTAAAQSAGLDADDVFHVEM
                     AVDEACSNVIEHAYEDQQGEIDLTCSTVEPGNLQIVILDHGQPFDPTTVPQPTFVDPS
                     QIDVMQEGGLGLYFMRKLMDDVHFEFVPSQGNKLTMIKRHHMVQ"
     gene            complement(2348..2680)
                     /locus_tag="VFF70_00020"
     CDS             complement(2348..2680)
                     /locus_tag="VFF70_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZY43109.1"
                     /translation="MNIETRQLKHVTVVTVVGRVDSATAPELEKKLHDLIEGNASQLI
                     LDLKQTEYMSSAGLRVLVSSLKLAKKNNGDLRLAQISTRVQEVLDLAGLTPVFHIHPD
                     LVEAVGSF"
     gene            complement(2776..4242)
                     /locus_tag="VFF70_00025"
     CDS             complement(2776..4242)
                     /locus_tag="VFF70_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY43110.1"
                     /translation="MLRVNRLEQIFNSDRKGVSTLATIALSACAALIVGLLVALLGGP
                     IALGAVLGLFVLAQILRQIEVGFAALAAVICLVPFAALPINIGFKPTFLDLIILALYG
                     VWMLERATGKLGRFVSTPLTIPVLVFLALALASFTAGLGYAPLTANIARHFAEIVLSV
                     VLYFLIADSVRQPVRLRSIVRMIIIGGSIAAVLAIVLYLLPHATSIDILSRLKLFGYP
                     EGPGVLRFINDDPSLPQRATGTSIDPNALGGLLIMTLTLGAPQLFSQKPVLPRFLILL
                     GLGCMGSALLLSFSRGSFLGTGVALFVLGVLRYRKLLLIMVVALTVILILPQTQAYVT
                     HFFQGVQGQDLATQMRFGEYSDALKLIQRYPVLGVGFTGSPDLDTYLSVASVYLLMAG
                     EMGLVGLAAFLILMLTLLVSAWRNRRAVANRSDVGAIWWGLHAALIGALVGGIFDHYF
                     FNLDFHHSVTLFWLYIGLAAVATRMVQSQPATIDQPAA"
BASE COUNT         1167 a         1272 c         1152 g          934 t
ORIGIN      
        1 atacaacttg ttgcgcgcgg tctgatcggc caatgaggca gcttgggcca acaccgctgt
       61 caaatcatca aacttcgtgc cgaactgagc gctggcacct ttgccgaagt gataatccaa
      121 gaagtcggtt tgatctggat agtccgcacc ccaacccaac aaatgcaagg acagattacc
      181 cttcgaagaa ttgtcaagga aggtgccaga ctcttgaacg tcgatggtga catcgatgcc
      241 caagttcttc ttcagctgcg cctggagttc ttcagcgacc ttaccgggcg ttggcagata
      301 gccgcgcacc acatcgcgat acgacagcgt gatgcctttc ggcatggtca cgccggccag
      361 caaggccttc gctgctacgg ggtcaaactt gtaccacggg tcgccttcgc aaccgccggg
      421 gatcgcgcaa ggcgtgaagt aatcagctac ggatgagccg actggatagt atttatcgac
      481 gatcgcctgt cgatcgatac ccatgccaat cgcttgccga accttttcat tgtcgaacgg
      541 cggcttggtg ttattgatgc ccaggtagaa cacgttcaac gcaacgcgcg gatacaactt
      601 caaggtcgca tcagcttcaa tcgctttgaa gtcattggga tcgggattgt ccatgccgtc
      661 aatttcgccc gacttcaagc tggtcaagcg ggcagccgct tctttgttcc acttgaagat
      721 aaccgtgccg gtcttgggct tgtcgcccca gtaattgggg ttgggcacta acgtcaaatg
      781 atcgccacgc acccattcct tgaccatgta cgcgcctgtg cctagagcat cttccagtgg
      841 cttgccgccg ctggctgtca aatgcgcagc ggattgaatg gccagcgaag caaaggcggc
      901 cttcgagggg aaggcaggat ctggcgtgca cattgtaaag acgacagttg tatcatcttt
      961 cgcttcgatc gacttcagtg tgccgcccca agtgcaatcc ggcgcagcct tggacagcat
     1021 cgtcacgggc actgcggtcg gggccgcagt gggagccggc ggcggagtgg tcggcttggg
     1081 ggccgtggtc ggtgcagctg tcggcgcggg agtcgcagcc ggctggcagg ccgagataac
     1141 cattgagact accatgagca ctgcgatgag tgcccacatc ttgcgagtgt gtgacatgtt
     1201 tttctcctcc tgagagaatc gttagaaaat tcgaatggtt aatttactaa actgaaccta
     1261 gtttgatcta tacgatcatc acctcctctc atcgtttttg aagggtacgc cttaaagtat
     1321 agattgcgcg gtcgagattg tcaatgaagt aggagcaaga aacggatcgc aactcaatcc
     1381 gatcggaata atcaaaccct aagacttgga attcacggcg ggttgagtca gggcgacaac
     1441 tgcctcagca acacgatcga acacacgaaa taaattagtg aaaccaatca tctcaaatat
     1501 ttcctgcgcg cgaacactca tggcggccag caccaaatcg ccgcctgcgg cttgggcttg
     1561 acgccgcgct tgaagtagag cacgcaagcc gctactcgag aggtatgaca cttcgctcag
     1621 atcgagaatt aagtagttgt ggccggcgct caactccgat tgcaaaaatt cttccaacgt
     1681 aggcaccgtc accgcatcca gtcgcccact gggaacgatg cgaaaaacta ctcggtcact
     1741 gccagttgcc tcttgaagaa ttttcacaat aatgtcctat ctgaatttcg attccggcct
     1801 agtgcaatta ctgcaccata tggtgccgct tgatcatggt gagcttgttg ccctgactgg
     1861 gcacaaactc aaagtgtaca tcgtccatga gtttccgcat aaaatagagg cccaacccgc
     1921 cttcttgcat cacatcaatt tgggagggat cgacaaaggt tggttgcggc accgtggttg
     1981 gatcaaacgg ctggccatga tctaagatca caatttggag attgcccggc tcgacagttg
     2041 aacacgtcag atcgatttcg ccttgctggt cttcgtacgc atgctcaatc acgttcgagc
     2101 aagcttcgtc gacagccatc tcaacatgaa acacatcgtc agcatccaag cccgccgatt
     2161 gagctgcggc cgtgacgaac tcggaaattc gggcgagtga ttcgaagcgg ccgggcacgg
     2221 ttaaatgtcg ttcctcgcgc accatcattg atacctgtca acgactctcg tcattctcga
     2281 cacaccgcct ctatcgcgtt aatcacgagc agccaaaagg tgcaataacg cgtccaggta
     2341 atcagaacta gaaggagcca accgcttcaa ctaaatcagg atgaatatgg aacacgggcg
     2401 tcagtccggc caaatcaagc acttcctgca cgcgcgtgga aatctgcgcc agccgcaggt
     2461 cgccgttgtt ctttttcgcc aacttcagcg acgacaccag cactcgcaga ccggcgctgc
     2521 tcatatattc ggtctgcttg agatccagga taagctgact agcattgcct tcgattaaat
     2581 catgcagctt cttttccagc tcaggcgcag tggcgctgtc gacgcggccg accacggtca
     2641 caaccgttac atgcttcaac tgccgagttt caatattcat gcaggtttct cctgtttcgt
     2701 gaagtgagca tcactaaaaa atgaagctaa gcgcgattat accatagcgt caattgattg
     2761 cgaatcgctt gcgccttacg cagcgggctg atcgatcgtc gccggttggc tctgcaccat
     2821 ccgggtcgcc accgccgcca agccgatata cagccaaaac aaagtgacgg aatgatgaaa
     2881 gtctaaattg aaaaaataat gatcgaagat gccgccgacc agcgcgccga tcaacgccgc
     2941 atgcagtccc caccagatcg ccccgacatc cgatcgattg gcaacggctc gacggtttcg
     3001 ccacgcgctg accaacaaag tcagcatcaa aatcaaaaac gcggcaagac ccactaaacc
     3061 catctcaccg gccatcaata aatacacact tgccacactg aggtaggtat cgagatcggg
     3121 cgagccagta aagcccacgc ccaataccgg atagcgctga atcaatttaa gtgcatcgct
     3181 gtattcgcca aatcgcattt gcgttgccaa atcttgaccc tgcacgcctt gaaagaaatg
     3241 ggtcacgtaa gcttgggtct gcggcaagat caaaattacg gttaaggcta cgaccatgat
     3301 caacagcaat ttgcgatagc gcagtacgcc caacacaaac agcgccacac cggtgcccaa
     3361 aaacgacccg cgcgagaacg acagcaacaa tgctgagccc atacaaccga ggcccagcaa
     3421 aatcaaaaaa cgcggcagca ccggcttctg actaaataat tgtggcgcgc ctaaggtcaa
     3481 agtcatgatg agcaagccac cgagtgcatt cggatcgatc gaggtgcccg tcgcgcgttg
     3541 gggcaaggac ggatcgtcgt tgatgaaacg cagcacgccc ggtccctcag gataaccaaa
     3601 caatttcaac cttgacaaaa tatcgatcga ggtggcatgg ggcagcagat acaaaacgat
     3661 ggcgaggaca gccgcgatcg acccgccgat gataatcata cgcacaatgc tgcgcagccg
     3721 aacaggctgg cgtacgctgt cggcgatcaa gaagtacagc acgacgctca acacaatttc
     3781 ggcgaagtgt cgggcgatat tggccgtcaa gggcgcgtaa cccaggcccg cggtgaagga
     3841 ggctaaagcc aatgctaaaa aaaccaggac gggaatcgtc aacggagtgc tgacaaaacg
     3901 acccaacttg ccggtggcac gttccaacat ccacacgcca tataaggcga gaatgatcag
     3961 atcgagaaac gtcggcttaa agccgatatt gatcggcagc gcagcgaacg gcaccaagca
     4021 aatcaccgcc gctaaggccg caaagccgac ttcgatctgc cgcaaaatct gagcgagcac
     4081 gaacaacccc aagaccgcgc ccaaggcgat cggtccaccc agcagcgcga cgagcaggcc
     4141 aacgatcagc gcagcacagg cgctcagcgc aatagtcgca agagttgata cgcccttgcg
     4201 atctgagttg aagatttgtt cgagtcgatt aacacgcaac atgttgtatc gattacttcc
     4261 aggagcggca taaggttcaa tgctcgttca tcgcctgatc cctgtaggtt tgcagtatag
     4321 cacacagacc attttcttca gcccaaatat aggcggtgtg cgcgtctcac ctgctcaact
     4381 gaagcagccc tcagaccgtt caggccggac gatcgatcat gcataagact caaacactta
     4441 cttcatgcga tccgtatcgc cggtgcagcc caattctttc atatccgaac tacctacgcc
     4501 gatgaaatta gtgtgacacc acaag
//
LOCUS       DASRTJ010000002         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75761,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000002 DASRTJ010000000
VERSION     DASRTJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75761"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFF70_00030"
     CDS             <1..1250
                     /locus_tag="VFF70_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZY43111.1"
                     /translation="LVLSACTPAATPVPTVAPTAVPPKPTVAAPSTAAPVVPTAAPTA
                     AAMSPFVSKPSSHVMDDGFVCPEPQNKIDLKSKDLNLFVWTEYIPQDIIDCFQLVYGI
                     KVNRDEYSSNEDMYAKLSQGSTNYDLVQPTDFIVGLMIRQGLLQKIDKSQLPNLAYFD
                     SNYLNLGFDPNNEYTIPYEAGTDSIVYNADTVATPPTSFADLFKPEYAGHMVVLDDSR
                     AIIGAVLLTLGYDVNTKDPKQLDEAKAKLAQLAKGIKLFDSDSPKTALIAGDVDLGIT
                     WTGEAFLAANENPAIKYIYPTEGAIVWQDNYAIPKNAPHLDAAYAWLNYSMQPDLFWL
                     MLRDFPYTNPVKGSLDYAKGNTLIVKDADGNDVTPDDLYQAYINSPVTNTPAADLKNG
                     HRIDDVGDALPLYDKIWTEIKGQ"
     gene            1369..2634
                     /locus_tag="VFF70_00035"
     CDS             1369..2634
                     /locus_tag="VFF70_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY43112.1"
                     /translation="MPSATDHIASVWGRIFNIMVDHGEGTYLYDTNGRRYIDFTCGIG
                     VTNTGHAHPQVVKAIQAQAAKMIHAQANIMYHQPMLELVDELLTIMPAPVLDSFFFSN
                     SGAEILEAAVKLAKQVNKRPSVIVFSGSFHGRTHLTMSMTTSKTIYRLGYQPLVPGIF
                     VTPFPYAYQLGMDEANATDYCIHELKRLLKSQVAPEEVACIVIEPVLGEGGYVVPPVA
                     FMQELRKICDQYGIWLVADEVQSGFGRTGKYFAVEHFSVVPDILVSAKGIASGLPLSM
                     LATRKELMNKWTPGSHGGTFGGNVIACAAAVATIRAIKQDQMLDNTVARGDQLVMGLK
                     KLQKDFSSIGDVRGKGLMIATEFTKNGEPDTDTTKAVVKAAFDRGLMLLTCGTYDNVI
                     RWIPPLVVTKEQIDEALTIFAAVLDTVTK"
     gene            2724..3497
                     /locus_tag="VFF70_00040"
     CDS             2724..3497
                     /locus_tag="VFF70_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY43113.1"
                     /translation="MTDRLLNKAALITGGTSGIGEAAAKLFAAEGACVAITGRRRELG
                     ENVVASIKQSGGEAIYIDADVVRSIDCARSIDQTLAAFKRIDILFNNAGFVAQGKLED
                     TSEADWDRSFEVNVKGVYLMTKLIVPVMRGQGGGVIINNASDWGIVGGPGYAAYSATK
                     GAVVLLTKTVALEVARENIRVNAICPGDTFVERWRVDERRNSSGDMAQRLKEMGEEMP
                     IGRVGTVAEIAKSVLFLASDDSSFMTGATLIVDGGNTAR"
BASE COUNT          853 a          966 c         1021 g          794 t
ORIGIN      
        1 gcttagtatt gtccgcctgc actccggcag caacgcctgt gccgaccgta gcgccaactg
       61 ctgtgccgcc caagccaacg gtcgctgcgc cgtccacggc tgcgcccgtc gtgccgaccg
      121 cagcgccaac cgccgcagcg atgtctccgt tcgtctcgaa gcccagcagc cacgtcatgg
      181 atgatggctt tgtctgtccg gaaccgcaga acaagatcga tctgaaatca aaagacctca
      241 atttgttcgt ctggacggaa tatatcccgc aggacatcat cgactgcttt cagctggtct
      301 atggcatcaa ggtcaatcgt gatgaatatt cctcgaatga agacatgtat gccaagttgt
      361 cacagggtag caccaactat gatctggtgc agccgacgga cttcatcgtc ggtctgatga
      421 ttcggcaggg actgctgcag aagatcgaca agagccagct gccgaacttg gcttacttcg
      481 attcaaatta tctaaacttg ggcttcgatc cgaataacga gtacacgata ccgtatgaag
      541 ccggcacaga ctcgatcgtc tacaacgccg ataccgtggc gacgccgccg acctcgttcg
      601 cggatctctt taagcccgag tatgccggac acatggtcgt gttggatgac tcgcgcgcga
      661 tcatcggcgc agtgctgctg accctgggct atgatgtcaa caccaaagac cccaagcagc
      721 tggatgaagc caaagccaaa ctcgcccagt tggccaaagg catcaagttg tttgacagcg
      781 acagccccaa gaccgcgctc atcgccggtg atgttgatct cggcatcacc tggaccggcg
      841 aagccttcct ggccgccaac gaaaatccgg ccatcaaata tatctatccc accgaaggcg
      901 cgatcgtttg gcaggacaac tacgccattc ccaagaacgc gccgcatctc gacgccgcct
      961 atgcctggct taattactcc atgcagccgg atctcttctg gttgatgctg cgcgatttcc
     1021 cgtacaccaa tccggttaag ggttcgctcg attatgccaa aggcaatacg ctgatagtga
     1081 aagacgccga tggcaatgac gtgacgcccg acgatttgta tcaggcatac atcaactcgc
     1141 ctgtaaccaa cacaccggcc gcggacttga agaatggcca tcgcattgat gatgtgggcg
     1201 atgcgctgcc tttgtacgat aagatctgga ctgaaatcaa agggcagtag ctaaacctga
     1261 tcggttttcg caccgaaggg ggttaggaga tgtgattaac tcctagcccc ctttgtgata
     1321 agtgtagaga tgttccgccg gaacgtctct acctaaacgg agaatatcat gccatctgcg
     1381 actgatcaca tcgcatccgt ctgggggcgc atcttcaaca tcatggtcga tcacggcgaa
     1441 gggacttatt tatatgatac caatggccgc cggtatattg acttcacttg cggcattggt
     1501 gtgaccaaca ccggccacgc tcatccacaa gtggtcaagg cgattcaagc acaggccgcc
     1561 aagatgatcc atgctcaggc caacatcatg taccatcagc cgatgctgga actggtcgat
     1621 gaactattga ccatcatgcc cgcgcccgtg ctcgattcat ttttcttcag caacagcggc
     1681 gcggaaatat tggaagccgc cgtaaagtta gcgaaacagg taaacaagcg gccgagtgtc
     1741 attgtgttta gcggatcgtt tcatggccgc acgcacttga cgatgagcat gacgaccagc
     1801 aagacgatct atcggctggg ctatcaaccg ctggtgcccg gcatcttcgt cacacctttt
     1861 ccgtatgcgt atcaactcgg catggatgaa gcgaatgcca ccgattactg tatccatgaa
     1921 ttgaagcggc tgctaaagag tcaggtcgcc ccggaagaag tcgcctgtat cgtgatcgaa
     1981 ccggtgttgg gcgaaggcgg ttacgtggtg ccgcccgtcg ccttcatgca agagctgcgt
     2041 aaaatttgcg atcaatatgg catctggttg gttgccgatg aagtccagag cggttttggc
     2101 cggaccggaa aatattttgc cgttgagcat ttcagtgtcg tgccggatat tttagtcagc
     2161 gccaaaggta tcgcctccgg cttgccctta agcatgttag cgacgcgcaa agaattaatg
     2221 aataagtgga cgcccggctc gcatggtgga accttcggtg gcaatgtcat cgcctgcgcg
     2281 gcggcggtcg ccaccatccg cgcgatcaag caagaccaga tgctcgataa caccgtcgcg
     2341 cgcggcgatc aattagtgat gggcttgaag aaactgcaaa aagatttttc atcgatcggc
     2401 gatgtgcgcg gcaaaggctt gatgatcgcc actgagttta cgaagaacgg cgaaccggat
     2461 acggacacga ccaaagctgt ggtcaaagcg gccttcgatc gaggcctgat gctgttgacg
     2521 tgtggcactt acgataatgt cattcgctgg attccgccgc tcgtcgtaac caaagagcag
     2581 atcgatgaag cgctgactat ttttgcggcc gtgctggata cggtgacaaa atgaaatcgg
     2641 acacggaaac acatggatta cgcggatgga aatttctaat tttatccgtg ttcatccgtg
     2701 caaaccgtgt ccaataattt cctatgactg atcgtctact caacaaagcg gcactcatca
     2761 ccggcggcac gtccggcatc ggcgaagcag cggcgaagtt gtttgccgcc gaaggcgcgt
     2821 gcgttgcgat cacgggccgt cggcgcgaac tcggcgagaa cgttgtcgct tcgatcaaac
     2881 aatcgggcgg cgaagcgatc tacatcgatg ccgatgttgt ccgatcgatc gactgcgccc
     2941 gatcgatcga tcaaacgctc gcggcgttca agcggatcga tatcttgttc aataatgcgg
     3001 gcttcgtcgc gcagggcaag ttggaagata cgagtgaagc cgattgggat cgttcttttg
     3061 aagtgaatgt gaaaggcgtg tacttgatga cgaagttgat cgtgcctgtc atgcgcgggc
     3121 agggcggcgg cgtgatcatc aacaatgcat ccgattgggg catcgtcggc gggccaggct
     3181 atgcggctta cagcgcgacg aagggcgcag tggtgctgct gacgaaaacg gtggcgctgg
     3241 aagtggcacg cgaaaatatt cgcgtgaatg caatttgccc cggcgatact tttgtcgagc
     3301 gctggcgcgt ggatgaacgc cgcaattcat cgggcgatat ggctcaacga ctcaaagaga
     3361 tgggtgaaga aatgccgatc gggcgagtag gcacagtggc cgagattgcc aagtccgttt
     3421 tgtttctagc cagcgacgat tcgtcattca tgacaggcgc aacgctgatc gtcgatggcg
     3481 ggaatacggc acgctaagtg gctctgggcg gagcgtcagc tccgccaaat ttcgaacgcg
     3541 agagctgacg ccctcgcttg agcagaaggg agtctaatgg aatacagatt attcggccga
     3601 acgggtgtga aggtttcgcc gttgtgttta ggtg
//
LOCUS       DASRTJ010000003         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_37951,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000003 DASRTJ010000000
VERSION     DASRTJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37951"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFF70_00045"
     CDS             <1..734
                     /locus_tag="VFF70_00045"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43114.1"
                     /translation="LRMCREIGNRRDECVSLGNLADVALYLGDHQAAHEYTEQAIKLA
                     RQIGNKISESWSLASQGLLLHQRKDDEAAYEYSQRALAIAADVGARHEQARAYTFSGH
                     ALIGLGKPIEAAEAYERALNLRRELGQRNLEFEALAGLARASYAQHKFDEARTHIESI
                     LLYLTDGSLDGTDEPIRVYLTCYTVLKAARDPRTEKTLITAYNILQERAERIQDDGIR
                     RSFLENVPAHRELIAELKHYDHNDK"
     gene            839..>3596
                     /locus_tag="VFF70_00050"
     CDS             839..>3596
                     /locus_tag="VFF70_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HZY43115.1"
                     /translation="MITTAQTRQLHWLQSVLKIQPGEGARVALMILYSAAAMGIVVTI
                     GYSVQDALFLSSLPTSDTPYLLILPAFTIGLSVLVYGRIAARIRLDHLVIGTACLLSI
                     LLLLVRVGLDTSFSKSFALLATSYVLIDLCVTLVLVQFWAFAGQVFNSREAKRLFGLI
                     ATGGTLVSMLAGLSLGPLSEWLGVPNLFFVIIGGLIVCGGCAWILGQWLYTPARVTET
                     AQSHRSEDKPASLQADLAGIRRSRLLLAIAGFIGLMSVFIFVAIYQFSIALQLNFGSD
                     DQGMVAFLGRFEFWINLIALLLQFFVISRVMKRFGLFAALLMLPIGMAIISIAGLLTG
                     AAFLVMVSLRGGELVLRRTFTDSAVNILYLPIAARLRQRAIELAEVLYQICAAIIGLA
                     ILALQNVPGWSYLNWSWLIVIGVGLWFGLWLWTRLQYQTALASNLKKRKIDFENTTID
                     ITDLATVKVLADTLRGSDAEYILHALSLITAAPNVNWDEYVAPLLSHPSPEVRVASLK
                     QIGRENNTAYVEAVADLLKAEDEGVRAAAIVAYCAIARPNAIDRIAPFLSETQSQVRG
                     AAVAGLIKYDGLAGRTQAELALDQMLHSPLPADRCEAARAIGLLTQLDANARLQMLLA
                     DHDRTVKIEAIRSIGIRQQRELLSQILQLLTDSHLAAAAAQALAEYGTTVESDLVALM
                     IDLQIDRSVRARVPDILQHIGTPTAFQILTQQLHDPDELIRSAVDTALRRLRPEHPEW
                     IVPVEWVNQALQIEIRYSYQFTMLRHDAQAESVDALLEDTLARRIQLSLDRIFLLLEM
                     LYPNEKLEPIRRGLDANQEYTRAAAIELLDNLVEHHIGELLIPLIEAPVAEMVEMAQR
                     RFTLQRWPLKHHLINLAEGQDRWLQACAIYQMGFTSAYELIEHVQAALKSDDPLLRET
                     AIAAG"
BASE COUNT          726 a          927 c         1041 g          902 t
ORIGIN      
        1 gtttgcgcat gtgccgcgag atcggcaatc gacgcgatga atgcgtgtcg ctgggcaacc
       61 tggccgatgt cgcgttgtac ttgggcgatc atcaggcggc gcatgaatat accgagcaag
      121 cgataaagtt ggcgcgacag atcggcaaca agataagcga gagctggtca ctggcctcgc
      181 aggggttgtt gctgcatcaa cggaaggatg atgaagccgc gtatgaatat agccagcggg
      241 cgttggcaat cgcggccgat gtcggtgcgc ggcatgagca ggcgcgggcg tatacatttt
      301 ccggccacgc gctcatcggc ctcggcaaac cgatcgaagc tgctgaagcc tatgagcgcg
      361 cgcttaacct gcgtcgtgaa ctcggtcaac gcaatctgga gttcgaagct ctagccggtt
      421 tagcacgtgc ctcatatgca cagcataaat tcgatgaggc gcgcacgcac atcgaatcga
      481 tcttgctgta tctgacggat ggcagtctcg atgggaccga tgaaccgatc cgggtttatt
      541 tgacgtgtta cactgttttg aaagcggcgc gtgatccccg cacggagaaa acattgatca
      601 cggcgtataa tattttgcag gaacgcgccg agcgcattca agacgacggt atccgccgct
      661 cgtttctcga gaatgttccg gcgcatcgtg aattgatcgc agagctgaag cactatgatc
      721 acaatgacaa atgaacaatg acaaatgaca aaaatatccg tgttcgtttc aatctgcggg
      781 gaaatttttt tgtgcagcat atgtttagtt cactaacgat gacttggcta acgatccaat
      841 gatcactacc gctcaaacgc gccagcttca ttggcttcag tctgtattaa agattcaacc
      901 cggcgaaggt gcgcgcgtgg cgttgatgat tttgtattcc gcggcggcga tgggtattgt
      961 cgtgacgatt ggctactcgg tgcaggacgc cttgttcctg agttcgctgc cgacctcgga
     1021 tacaccctat ttgttgattc tgccggcgtt cacgatcggc ctgagcgtgc tggtgtatgg
     1081 ccgcattgcc gcccgcattc gcctcgatca tttagtcatc ggtacggcat gcctcttaag
     1141 catcttgttg ttgctcgtgc gggtcgggtt ggacacgtcc ttcagtaaat catttgcgct
     1201 gcttgcgaca agctatgtgc tgatcgatct gtgtgtcacg cttgtgcttg tccagttctg
     1261 ggcttttgcc gggcaagtat tcaattcgcg cgaagccaaa cggctgtttg gtttgatcgc
     1321 gaccggcggc acactggtca gtatgctggc cggtctgtcc ttgggtccgt tgtctgaatg
     1381 gctcggcgtg ccgaatttat ttttcgtgat catcggtggg ctgatcgtgt gcggcggctg
     1441 cgcgtggatc ctcggtcaat ggctctacac tccggcgcgc gtcacggaaa ccgctcaatc
     1501 gcatcgctct gaagacaagc ccgccagctt acaggcagat ttggcgggca tccggcgatc
     1561 acggctgctg ctggctatcg ccggttttat cgggttgatg tcggtcttta ttttcgtcgc
     1621 catttatcaa ttctcgattg cgctgcagct caacttcggt tccgatgatc aaggcatggt
     1681 tgccttcctg ggccgatttg aattctggat caatctgatt gccttgctgc tgcaattctt
     1741 tgtaatcagc cgcgtcatga aacgtttcgg cttatttgcg gcgctgctca tgctgccgat
     1801 cggcatggcg atcatctcga tcgcgggttt gttgaccggc gccgcctttc tggtgatggt
     1861 tagcttgcgc ggcggcgaat tagtgttgcg acgtaccttt accgactcgg ccgtcaatat
     1921 cttatatctg ccgattgcgg cgcgtttgcg ccagcgggca attgaactgg ccgaagtgtt
     1981 gtatcaaatc tgcgccgcga ttattggatt ggcgatcttg gcgttgcaaa atgtgccagg
     2041 ctggtcgtat ctcaattggt cgtggttaat cgtgatcggc gtcggcctgt ggtttggctt
     2101 gtggctgtgg acgcggctgc agtatcagac tgcgctggcg tccaatttga agaaacgcaa
     2161 gatcgatttt gaaaatacga caatcgatat taccgatttg gcgacggtga aagtcttggc
     2221 agacacgctg cgcggctctg acgccgaata tatcctgcac gcgctcagcc tgattactgc
     2281 agccccgaac gtaaattggg atgaatatgt tgcgcctctg ctgagtcacc cctcgccaga
     2341 agtgcgcgtc gcttcattaa agcagatcgg tcgtgagaac aacactgcct atgtcgaggc
     2401 cgtcgccgat cttctcaaag cggaagatga aggtgtgcgc gcggcggcga ttgtagcgta
     2461 ctgcgctatc gcccggccga acgccatcga tcgtatcgcg ccgttcttgt ctgagacgca
     2521 atcgcaggtc cgcggcgcag ctgtggctgg cttgattaaa tatgatggac tggccggccg
     2581 cacgcaggcg gaactggcgc tcgatcaaat gctgcatagt ccactgccag ccgatcgatg
     2641 tgaagcggct cgcgcgattg gcttgctcac tcaattggac gccaacgccc ggctgcaaat
     2701 gctgctagct gatcatgatc gcaccgttaa aatcgaagca atacggtcga tcggaattcg
     2761 acagcaacgt gaactgctgt cccagattct gcagctgttg actgacagtc acttggccgc
     2821 agcggcggct caggccttgg ctgagtatgg gacgaccgtt gaatctgatc tggttgcgtt
     2881 gatgatagac ctgcagatcg atcgttcggt gcgagcgcgt gtgccggata ttttgcagca
     2941 tattggcaca ccgacggctt tccagatttt gacgcagcag ctgcatgatc cagatgagct
     3001 catccgatcg gccgtggaca ccgcactcag gcgcttgcga cctgaacatc cagagtggat
     3061 cgttccggtc gaatgggtca atcaggcgct gcagattgaa attcgatata gttaccagtt
     3121 caccatgctc cggcacgatg cgcaggctga atcagtcgat gcgttgttgg aggatacgct
     3181 ggcccgccgg atacagctgt cactcgatcg gatctttttg ttgctcgaaa tgctgtatcc
     3241 taatgagaaa cttgagccta tccgccgtgg gctggatgct aatcaggaat acactcgcgc
     3301 cgccgcgatt gagctgttgg acaatctggt cgagcatcac atcggcgagc tgttgattcc
     3361 gttgattgaa gcgccggtgg ctgaaatggt cgagatggct caacgtcgat tcactcttca
     3421 gcgttggccc ttgaagcacc acttaatcaa tttggctgaa ggtcaagatc gctggctgca
     3481 agcctgtgcg atctatcaaa tgggttttac atcggcctat gaattgatcg agcacgttca
     3541 agccgcgctg aaatccgacg atccgctgtt gcgcgaaacg gcgatagccg ctggca
//
LOCUS       DASRTJ010000004         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_189307,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000004 DASRTJ010000000
VERSION     DASRTJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189307"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFF70_00055"
     CDS             complement(<1..728)
                     /locus_tag="VFF70_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZY43116.1"
                     /translation="MTTYTYDSANRMVSAGGVAYTWDNNSNLTNDGSTLYRFDQANRM
                     ISTTLSGATTLFNYNGDGVRLKQVTAGVPTTYTQDIVAPLPVVLQAKTGTVATQHLFS
                     MGTRPIGQYTSSAWQYLLPDGLGSVRQIADASGNVLLSESYEPYGKLLSSSGSASSIF
                     AYGGEQIDTSGLIYLRARYMQPGLGIFLSHDPYSGDVMRPGSMNGWNYTEGNPVNRLD
                     PTGWYAYFTDEEAGAITLLTLWFL"
     gene            complement(931..1674)
                     /locus_tag="VFF70_00060"
     CDS             complement(931..1674)
                     /locus_tag="VFF70_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZY43117.1"
                     /translation="MATLYLGTQGFSYQDWVGNFYPPHTHPENYLTHYAKHFRALELD
                     NTFYTVPRSTSIQAWYDHTPPDFIFSAKFPQSITHAKQLINAEAETHTFLEMMSGLKE
                     KCGPLVLQFKYDFDPKSANDLDRYLAQLPKSFRYVIEFRHTGWLQDRYFDLLSQHRAA
                     FCLHDLYYMPKIARITTDFTYIRWLGNRKQLKHFERIQIDRSTEQAWWSGVAKDALRH
                     GIDVFGFFNNYWAGHAPSSAEMFLKQLVG"
     gene            complement(1756..>2089)
                     /locus_tag="VFF70_00065"
     CDS             complement(1756..>2089)
                     /locus_tag="VFF70_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43118.1"
                     /translation="YPGDHILGEVQVIGGDAKTIKTATVDLLWREKGEAHDFVDHTEG
                     LNVRVEIDPVQLTSGQLFPIDIPIPLDADPSFVSQHASMTRLVRAELKLADDKSLIAE
                     TIIRIGTK"
BASE COUNT          505 a          546 c          506 g          532 t
ORIGIN      
        1 tcaagaaacc aaagggtcag caaagtaatt gcaccggctt cttcatcagt aaaataggcg
       61 taccaacctg tcggatcaag tctattaacc ggattcccct cagtgtagtt ccacccattc
      121 atcgatccgg gtctcatcac atccccacta tacggatcat gtgacaaaaa tattcccaag
      181 cccggctgca tatatcttgc ccgtaagtaa attaacccgc tggtatcgat ctgttccccg
      241 ccgtaggcga atattgaact tgcagatcca ctactcgaca acagttttcc gtaaggttca
      301 taactttcgc tcaacaagac atttccactt gcatcagcaa tttgtcgcac cgatcccaac
      361 ccgtccggca ataaatactg ccatgcgctc gaagtatact gcccaatcgg tcgtgtgccc
      421 atgctgaaca ggtgctgagt cgcaaccgtg ccagtcttcg cttgaaggac aacaggcaac
      481 ggggcaacga tgtcttgcgt gtatgtcgtt ggcacaccag cagtcacttg cttcaatctc
      541 acgccatcgc cgttgtagtt gaagagcgtg gttgctccgc tcaacgtggt gctgatcatt
      601 cgattggctt gatcgaagcg atacagcgtg ctgccatcat tggtgagatt gctattgttg
      661 tcccaagtgt aagctacgcc acccgcactc accattcgat tcgcagagtc gtaggtgtaa
      721 gttgtcacga cggttgaggt gatatgctgg ctctaacgcg catgcagggc agaaatatgc
      781 ttccactatc tttatgccag cgtaaatgca aaatccaaaa aatgcactta ttgctcccgc
      841 ttctgcataa gcgggactga atgcaggcag ttgaagacga cgctcgtttt gctgtgttac
      901 ttttcccctg tcactcatca cttctcttaa tcaacccact aactgcttta aaaacatttc
      961 tgcgctcgac ggtgcgtgcc cggcccaata gttgttgaaa aagccaaaga catcgatacc
     1021 atgccttaat gcatcttttg ccacgccgct ccaccaagcc tgctctgtgc ttcgatcgat
     1081 ctgaatccgc tcaaaatgct ttagttgctt gcgattgccc agccagcgga tgtaagtgaa
     1141 gtccgttgtg atgcgggcga tcttcggcat gtaatacaga tcgtggaggc agaacgcggc
     1201 tcgatgttgg ctgagcagat caaagtatcg atcttgcagc cagcctgtat gacgaaactc
     1261 aatcacatat cgaaacgatt tggggagttg agctaaatac cgatcgaggt cgttggcgga
     1321 cttgggatcg aaatcgtact tgaattgcag caccagcggg ccgcactttt ctttcaaacc
     1381 agacatcatc tccagaaatg tgtgcgtctc ggcttcggca ttgatcaatt gcttagcgtg
     1441 ggtgatcgat tgcggaaatt tggcgctgaa aataaaatca ggcggggtat ggtcatacca
     1501 cgcctgaatg gaagttgatc gcggcaccgt ataaaacgta ttgtcgagtt ctagcgcgcg
     1561 aaagtgtttg gcgtagtgcg tcagataatt ttcagggtga gtgtgcggcg gataaaaatt
     1621 gccgacccaa tcttggtaag agaatccttg cgtgccaaga tagagcgttg ccatatatca
     1681 tctgtgtcgc cctgagcaaa gcgaagggtc tcgcacactt ttccgagatg cttcgctcag
     1741 catgacaatc aagaattact ttgtcccaat gcggatgatc gtttcggcta tcaaagattt
     1801 atcatcggcc aatttgagtt ccgctcgaac gagccgcgtc atcgacgcat gttgactgac
     1861 gaacgacggg tcggcatcca gcgggattgg gatatcgatc ggaaacagtt ggccgctggt
     1921 tagctgcacc ggatcgatct ccacccgcac attcaggcct tccgtgtgat cgacaaaatc
     1981 gtgcgcctcg cctttttcgc gccacagcag atcgaccgtg gcggtcttga tcgtttttgc
     2041 atccccgccg atgacttgca cctcgcccag aatgtgatcg cccggataa
//
LOCUS       DASRTJ010000005         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75899,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000005 DASRTJ010000000
VERSION     DASRTJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75899"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFF70_00070"
     CDS             <1..184
                     /locus_tag="VFF70_00070"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZY43119.1"
                     /translation="AFTDRVGEIAETEGHHPDILTQWGKVTIWWSTHKIKNLHRNDFI
                     MAAKTDDIAQEESFAS"
     gene            884..2530
                     /locus_tag="VFF70_00075"
     CDS             884..2530
                     /locus_tag="VFF70_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZY43120.1"
                     /translation="MATIDGLEAWTRASHGLTILIGVNPPSPSIAFNLPLLRADAPFV
                     TVPYGLMTQLLSIASRLPGARAALRASAHSLSFILPQSLGDGYQHTSNDSAQPAAALA
                     DLLQRLSGDAPLVIQLNDLHRADEPSLKLLREVARLIEPQPILIVGTYRADLQHTATL
                     SEMLNDRSFPQLTCGPVWSNDLSIMLDGLFGRKVTLGSMIDAIKNDPRSMLLGGAAVI
                     EACRLQGRWTDGLMLSERAIELAAKHEAHYLHAIVRSIRGDILIGLGQAAEAEAEFDQ
                     VAESAAIKRDNDLVAAAAWGSHKARSMLSVKRLALPGALKRWRTQVRSSNDPLTAAAL
                     LGDIIAHLIESGQLGEARSWQHDLEMLVDYSGHPMARYAAAYCLGEFLTYTNDWRSAS
                     GAYRAAMQHAGVMHDILSEARANSQLALALLELGDLESKNEGRERLSIAHSVLSRLNA
                     RPDIEKSEAGAKRFGLRPRQRRPSMSNRTPGSLTRREREVLGLVMNGMTNRQIAQRLT
                     ISEKTAEGHVGNILAKLSCSSRMKAAELARATGLLEGVPA"
     gene            complement(2629..3438)
                     /locus_tag="VFF70_00080"
     CDS             complement(2629..3438)
                     /locus_tag="VFF70_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43121.1"
                     /translation="MLSNLSRRLTYLNALLYATLGTLLFIQPEQLAPIFAWKVTPFMT
                     MTIGGWCLGNAWLAFLAARRWRWELVYSSLLYLWIFGLFEILIVIAFNDKLQLGQPIA
                     WLYLFTLLINIVSAIVGLIEWLRVRPTLPTSNTPRSGLLRGFAILFVIAVAFLGGYGL
                     LARIGDPGTSGQVFPEVMSLFTLRSFGAFYLALAIGVVPLIWARDRRSVLSHGFLAFG
                     FLIFVTLAALVYVRLFNFSAHPFQLLYLGAYFSVAAVTIVFLLKGGTGTRT"
     gene            complement(3514..>4254)
                     /locus_tag="VFF70_00085"
     CDS             complement(3514..>4254)
                     /locus_tag="VFF70_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZY43122.1"
                     /translation="VLLIVRGESLPRGRSLFQLIGMGALGYVGPSFSYLTAIKYASAG
                     LVALLLYLYPAFVAILSATFLKEKFTRLKIAALIIATLGAALTANPQGGSWTGILLAI
                     TAAAIYSVYIIVGTTVLKRVSAIQSSTVIFAGAGVVFSTLAIINGPQLPHSSAGWTAL
                     IAIILVATILPVVAFLAGLKRIGPTNASLLSTLEPVVTVLLAAWLFGEVLQSAALMGG
                     GLILIAVILLTRSELHQADRQPDQIDLT"
BASE COUNT         1087 a         1091 c         1149 g          927 t
ORIGIN      
        1 ggcattcact gaccgtgttg gcgagatcgc ggagactgaa ggtcatcatc cagacatatt
       61 aacacaatgg gggaaggtta ctatctggtg gtcaacgcat aaaatcaaaa atctgcatcg
      121 caatgacttc atcatggccg cgaagaccga tgacattgcg caggaagaat catttgcatc
      181 ctgatcggca gattgtgcct acgcgctagt acactatcat gccattgcgt aatcggatat
      241 tcgcacggcg gtgccaaata gtgcacgcgt tagctcatct ccatgcgata aactgagaca
      301 aactgttatt gcctttttcc ttggtgtgat gacagggtgg acgcacaaga acattggttt
      361 gcatcgcaaa gatttcatta ggacctcgaa gaccgatcgg gtggaggcga tttccaatgg
      421 atcgccgatc ggattctggc gccttccgga cactcatcga ttcagcattt cagatcgatc
      481 aaattgatcg acgttggagt gatcgaggga acactcctcg ccttataaac gtctcatcta
      541 gtgtgtcaac caatttaata tatttgcaac gaatttttca cgattgcgtt atcactgtac
      601 tattcgtgcg taatacgtga agacaatttg atcgcttatg tgctatcagg ccgtgagcgt
      661 tgtttgcacg tagcgcatat gtaggatcag cgtcgtgtct tttcattcac ttctcatcta
      721 atattcatct gcaaggctta tactcaagtc gtattaagtt tggctaaagg agaacagggg
      781 gatgccactg gataccattc gtaagaatcg catgatccta gcgcctcgcc gttttatcgc
      841 accccaacct actgaaactg cgcgatcact gcgccggatg gaaatggcta ccatcgacgg
      901 attggaagcc tggacgcgcg ccagtcatgg cctcaccata ttgatcggtg tgaatccacc
      961 cagcccatcg atcgcattta atctgccact tctgcgtgcc gatgcgcctt tcgtcaccgt
     1021 accttacggg ctcatgacgc aattgttgtc aatcgcttcg cggctgcccg gcgcccgggc
     1081 agccctgcgc gcttctgctc actcactcag ttttattttg ccccaatcac tcggcgacgg
     1141 atatcaacac acatcgaacg attctgctca accagccgcg gcgttggccg atctattgca
     1201 acgattatcc ggggatgcgc cattagtgat tcaactcaat gacttgcacc gcgcggatga
     1261 gccaagtttg aaactgttgc gcgaggtcgc tcgcttgatc gaaccgcaac cgatcttgat
     1321 cgtgggtacg tatcgcgccg atctacaaca cactgcaact cttagcgaga tgttgaatga
     1381 ccgatcgttt ccgcagctaa cctgcggccc cgtctggtcg aatgatctct cgatcatgtt
     1441 ggacggcttg tttggccgca aggtaacctt aggttcgatg atcgatgcca tcaaaaacga
     1501 tccgcgttcg atgctattag gcggcgcggc ggtgattgaa gcctgccgct tacaaggccg
     1561 ttggacggat ggcttgatgt tgtccgagcg cgcgatcgaa ttagcggcga agcacgaagc
     1621 tcattacttg cacgcgattg tgcgttcgat tcgcggtgac atcctgatcg gtttggggca
     1681 agccgctgaa gccgaagccg aattcgatca agttgcagag tcggcggcaa ttaagcgcga
     1741 taacgatttg gttgcagctg ccgcttgggg ctcgcataaa gctcgatcga tgctgtcagt
     1801 gaagcgtctg gctttgccgg gagcgttgaa gcgctggcgc acgcaggttc gatcgagtaa
     1861 tgatccattg acggcggccg cgctgctggg cgatatcatt gcccacttga tcgaaagtgg
     1921 ccagctaggc gaagcgcgat catggcagca cgatcttgaa atgttagtcg attactccgg
     1981 ccatcccatg gcgcgatatg ctgcggccta ctgcttgggc gagttcttga cttataccaa
     2041 tgattggcga tccgcctcgg gcgcttatcg tgcagcgatg cagcacgctg gagtgatgca
     2101 cgatattttg agtgaggcac gagcgaatag tcaattagct ttggcattgc ttgaactcgg
     2161 cgatctggaa agcaagaacg aaggccgcga gcgattgtcg atcgcccatt ctgtgttgtc
     2221 gcgcttgaat gcgcggccgg atatcgagaa gagcgaagcc ggcgcaaagc gatttggtct
     2281 caggccgcgc cagcgccgcc cgtcgatgag caatcggacg ccgggcagct taacgcggcg
     2341 cgaacgtgaa gtgctagggc tggtgatgaa tggcatgacc aatcgacaga tcgctcaacg
     2401 cttgacgatc agtgagaaga ctgctgaagg ccacgtcgga aatattttag cgaagttgag
     2461 ttgcagcagc cgcatgaagg ccgccgaact cgcacgtgcc acgggtttgc ttgaaggcgt
     2521 gcccgcgtag gaacgccgag ataaaacagg caagaccatg tgtcgctact caaaaagaaa
     2581 acttccgaag tctcacagac ttcggaagtt ttttattcga aataaagtct aagttcgcgt
     2641 accggtcccg ccctttagca aaaatacgat tgtgacggcg gccacgctga agtacgcgcc
     2701 gagatagagc agttgaaacg gatgcgcgct gaagttgaat aaacgcacat agactaaggc
     2761 ggcaagcgtg acgaagatca gaaaaccgaa agccaagaag ccgtgactta acaccgagcg
     2821 tcgatctcgc gcccaaatga gcggcaccac gccgatcgcc aacgcgagat agaacgcgcc
     2881 gaaactgcgc agggtgaaca acgacatgac ttccggaaat acttggccgc tcgtccccgg
     2941 atcgccaatt cgggcgagga gaccatagcc gccaagaaaa gcaacggcga tgacaaacaa
     3001 aattgcgaaa ccgcgcaaca aacctgatcg tggcgtgttg ctcgttggca atgttggtcg
     3061 aactcgcagc cattcaatca agccaacgat cgcgctgacg atattgatga ggagggtgaa
     3121 cagatacagc caggcgatgg gctgacctaa ttgcaacttg tcattgaagg caataacgat
     3181 caaaatttca aataatccga agatccacag atacagtagc gaagagtaaa ccaattccca
     3241 acgccagcgc cgcgcagcga gaaaagccag ccacgcattg cccaagcacc acccgccgat
     3301 tgtcatcgtc atgaacggtg ttactttcca ggcaaaaatt ggcgcgagtt gctcaggctg
     3361 aatgaacaac agtgtgccca aggtggcgta caacaaggca ttcaggtaag tcaagcgacg
     3421 tgagaggtta gacagcatat tgatctcctc ttagtaactt gtggggttag cgcaaccgat
     3481 cagctgacaa taagtatagc gtcgatcgat ccatcaagtc aaatcaattt gatcgggttg
     3541 gcgatctgct tgatgcagtt cactgcgggt caataatatc acggcgatca aaatgagtcc
     3601 gccacccatc aaagctgcag attgtagaac ttcgccaaaa agccaggcgg ctaataacac
     3661 cgtgacgact ggctctagtg ttgacagcag cgaagcgttt gtcgggccga tacgcttcag
     3721 tcccgcgaga aacgcgacaa cgggcaaaat tgtcgcaaca aggatgatgg cgatgagcgc
     3781 ggtccaacct gctgaactgt gcggcagctg cggcccattg atgatggcca acgtcgagaa
     3841 caccacaccg gctccggcaa agatcacggt cgaagattga atggctgaaa cgcgtttcag
     3901 aacggtggtg ccaacgatga tgtagaccga atagatcgcg gcggccgtga tagccaacag
     3961 gatacccgtc catgatcccc cttgcggatt ggctgtcaac gcagcgccga gtgtagcgat
     4021 aatcaatgca gcaattttta agcgcgtgaa tttttctttg aggaatgtgg cggagaggat
     4081 cgccacgaac gcgggataaa gatacaacag cagcgcgact aaaccggctg aggcatattt
     4141 aatggcagtg agatatgaaa acgatggacc gacatagccc agcgcgccca tcccaattaa
     4201 ctggaataac gatcgaccgc gtggcaatga ctcacctcga acgatcagca acac
//
LOCUS       DASRTJ010000006         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_151497,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000006 DASRTJ010000000
VERSION     DASRTJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151497"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="tilS"
                     /locus_tag="VFF70_00090"
     CDS             <1..932
                     /gene="tilS"
                     /locus_tag="VFF70_00090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZY43123.1"
                     /translation="SYLIRPLLETPREAIKDYCQQNNLQPRIDATNADTTYFRNRLRH
                     DLLPLLETYNANIRSILRRTASVIAAEYEALETHVDAAWHETIIEEGDAIIFHLAKFK
                     AQPLAIRRSLLRRSIQQLRPPLRDVDFVNIEDAIDLIERAETGDRSTLPQELMLEVGY
                     DSITISMANETILPDWPLLSEETGAIPITVPGSTRLTNSQWSIEATVLPEVENFREDM
                     WSTHLDADSLSGPLSIRPRTSTDRFQPQGMRSPIGLKDWMINAKIPRAVRDRLPLIVS
                     GDQIVWVAGFRVGQPFIVREDSKQIVKLTFSKD"
     gene            991..>1522
                     /locus_tag="VFF70_00095"
     CDS             991..>1522
                     /locus_tag="VFF70_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY43124.1"
                     /translation="MDNPDLILTSPITAADRSHLNHGYAIAITGVALWSTTGIFISYL
                     LKNFTLAPMTLAFWRNIIVIIALGLGLFVFKRSLLRVQKRDRWFLILYGLSLTLMNGL
                     WTFSVAYNGAAVSTVLVYASPGITAVAGKFFFKERLTWLRIAAVIASLIGSALVARAI
                     DPAQWNLNAIGIVIGIL"
BASE COUNT          361 a          406 c          383 g          372 t
ORIGIN      
        1 gctcttatct cattcggcca cttctcgaaa caccgagaga agccattaaa gactattgcc
       61 agcagaacaa tcttcagcca cgcatcgatg cgacgaatgc cgacacgaca tacttccgca
      121 atcgattgcg tcatgatctg ctaccactgc tcgaaacata caatgccaac atccgatcga
      181 tcttgcggcg cacggcctcc gtcatcgcgg ctgagtatga agcactggag actcacgtcg
      241 atgcagcctg gcatgaaacg atcattgaag aaggtgatgc gatcattttt catttggcaa
      301 aattcaaagc acagccgctg gcgattcgtc gatcgttgct gcgccgatcg attcagcaac
      361 ttcgtccgcc gttacgtgac gtcgatttcg tgaacatcga agacgcgatc gatctgatcg
      421 aacgcgcgga aaccggcgac agatcaactt tgccgcaaga attgatgttg gaagtcggtt
      481 atgattcaat cacgatctca atggccaacg aaacgattct tccggattgg ccactcttat
      541 cagaagagac aggtgctatc cccatcactg taccgggatc aacgcggctg acgaattctc
      601 aatggagcat tgaagcaaca gtcctcccgg aggttgagaa ctttcgggag gatatgtgga
      661 gcactcatct cgacgccgat agcctatcgg gtccattgag catccgcccg cgcacttcga
      721 cggatcgatt tcaaccgcag ggcatgcgat caccaatcgg gctaaaagat tggatgatca
      781 atgctaaaat tccgcgcgct gtgcgcgatc gattgccgtt aatcgtctcg ggcgatcaga
      841 tcgtatgggt ggccggattt cgcgtgggac agcccttcat cgttagagaa gattccaaac
      901 aaattgtcaa gttgactttc agcaaagatt agcaaaggac acgcgatacg cgacgagtga
      961 aatcacgcct cacgtatcgc acatcaattc atggataatc ccgatctcat tcttacctct
     1021 ccaattactg ctgccgatcg atctcatcta aatcacggct acgcgattgc gattactggc
     1081 gtggcgctct ggtcgacgac cggtatcttc attagttacc tattgaagaa cttcacgctc
     1141 gcgccgatga ccttggcatt ttggcgcaac atcattgtca tcatcgccct gggcttgggt
     1201 ctattcgtct tcaagcgtag tttgctgcgc gtgcagaaac gcgatcgatg gttcttgata
     1261 ctgtatggct tatcgctgac attgatgaat ggactgtgga ctttttcagt cgcgtataac
     1321 ggcgcggcag tatccaccgt gctggtctat gcttcgccgg gcattaccgc cgttgccggg
     1381 aaattcttct tcaaagaacg cttgacgtgg ctgcggatcg ccgcggtgat cgccagcttg
     1441 atcggcagcg cgttggtcgc acgcgcgatc gatccggctc aatggaattt gaatgcgatc
     1501 gggatcgtca tcggcattct at
//
LOCUS       DASRTJ010000007         5810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_227336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000007 DASRTJ010000000
VERSION     DASRTJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VFF70_00100"
     CDS             <1..1467
                     /locus_tag="VFF70_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43125.1"
                     /translation="VYLLLRALNLSSREDARRTPTTTRMTGLARAIVTAATLYTHFIG
                     FFLLPFEALLIVLVIGRQQRREVILAGSGLLILALPIIAYGLSRAAGHDANFGFRPLD
                     SIAEELWSTFIVGRSNEFFQPWWAVAPGLLLLVLGSLYGLINRSARSSTLLALGYLII
                     PLLLFYAATFTRPLYTGPRHLILIAPPFYLLMACGLDAIWKRSRLIAVAALLWSVALS
                     GIWLYRQYIDPADTKEDIRSMAQLISARVTPQDAVILEGAVGSFTFDYYYTGSAPRSF
                     IPVFPSSDVEGAITALNARAQTADRVWLVTDPPPENISSNALDQWARGHLLRLDHQRF
                     PAMWLGSAYQLYTAHFPIYAALPIDAMPRNIQWLTAALRLAGAGSIVVSPDRGVVTTA
                     LYWRIDASPTSDFIFTQRLIDDTGAEWGLQIGAAFDNWSAAQWPIGKLIKQETQIDLP
                     HGLPAGHYRLLVSVADKSGQPFTLLSGLGEIEAAQVDN"
     gene            1470..3764
                     /locus_tag="VFF70_00105"
     CDS             1470..3764
                     /locus_tag="VFF70_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43126.1"
                     /translation="MTQSRITHHASSFLSVKTIVITFTVIASIYSISTPIFEASDELW
                     HYPLVQWLSKGNPLPLQDAKNVGPWKQEASQPPLYYTLMGWATFWIDTRDMPQVRIEN
                     PHVDNGVITTDGNINLVVHNPDRESFPWRGTVLAVHLIRFLSVLMGAVTVWLTYRIAL
                     ELFPDRSYLALGAAAVNAFTPMFVFISGAINNDNLTMLLCSLSLLLMIKRLREEQGAG
                     GRKFSFRLGRWLPLGIVIGLATLTKTSALGLLPITAITVVIVAWRRGSWPEFFAGAIA
                     TAVPVLLIAGWWYLRNIQLYGDVLGLSAFIDVLGRRAAPASLLQLWGERWGFMLSYWG
                     LFGGVNVPLDNWVYHILNALAVLAFIGAIIYQVRMTMDWFRSDPIHTWRDFRFKLRDY
                     LQGRAPLFIVGLFGLIVVVSLTQWATITWSSQGRLVFSAISMWSIFSVVGLATLFGSR
                     FARLAIAATSVLMFLITLLAPFTTITPAYARSLPVQSLQPPAISFDVTFNHQLKLIGA
                     DFKSTSAQPGGSIEIILYWQASAPIDKNYSTFVHLLDENEIVVAQRDMFPGQGLWPTS
                     QMKVGDIIPSRYVINLPATAYAPDKLIFEVGVYDYASQTRLLMLDGADNLRFGSIDLQ
                     SVAGDVPNPIDYNFGDQIDLVGYSLDRRSAAPNDALQLTLYWRARSKVPIDYTVFTHL
                     LQPPQTIWAQQDKPLSPPSSSWTIGQTISQTYDLHLKPDVSSGVYEIEVGLYDSANFD
                     RLRIVADDGRITDNFVLLSKVRIK"
     gene            3818..5674
                     /locus_tag="VFF70_00110"
     CDS             3818..5674
                     /locus_tag="VFF70_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HZY43127.1"
                     /translation="MKVSRQNFIPWWTNWAAPFMLLIFVLASIFLAFRQHAAFQTNLY
                     DLGLYAQRVWNLSRGAGFTTSLGLTNFLSGHFAPLLALIAPTFLIWPDARMLMIIQAI
                     ALTMSIVPGYLILRIRYPLMAPALVLAFVFSPLLHQTSATEFHGIILAAPFMAWAFYA
                     LYMRRTRMMLVALAFAVLAREDMGLYVACFGLFMLICRKGQRLIGLALAAFGGAWVIV
                     VINWVMPIFGHTYRHFNAFSALGGDSVTEMIANVIHDPIRVARIIFTSAKLAALAKFI
                     LPLAGLPIVALGYPLLWLPVVAVYLLSNATGSGLLNSWRVAPFLPLLWGSIAALFARL
                     RPRWAIGGMAVLMLATLIGYLTLSPFPGGGQFDATLYQVNQHTQIGEQIVASIPPAVS
                     VAAQDGLAAHLSTRAQMRLFPLYSLANTPEFIVLDDKATDFYPQTADEFKLALLNLQM
                     NPQFDVVHEQDGYFVFRSTGGVNQSLQPTSLTYSSTLKLYGFDLAQSAGSNPFSAVSG
                     NADHGDILRVSLYWTALVPMPNHLSISVRVNDAAGNIIAQDDSWPARGQIPTPLWEIG
                     RSIRDVHYLNLPDSALQELLTLKVIVYAADTLQPLEPVEGYVLSALLGTQPK"
BASE COUNT         1149 a         1514 c         1560 g         1587 t
ORIGIN      
        1 gtgtatctct tgcttcgagc gctgaatctt tcctcgcgcg aagatgctag acggacacca
       61 acaaccactc gaatgactgg gcttgcgcgg gcgatcgtca ccgccgcaac tctctatacg
      121 cattttatcg gattcttcct gttgccgttt gaagccctgc tgatcgtatt ggtcattggc
      181 cgtcagcagc ggcgcgaggt tattctggcc ggaagcggat tgttgatctt ggcgctgccg
      241 attatcgctt atgggttgtc gcgcgcagct gggcacgatg ctaatttcgg ctttcgacca
      301 ctggacagca ttgctgaaga actatggagc acattcatcg tcggtcgatc gaatgagttc
      361 tttcagccgt ggtgggcggt tgcgcccggc ttattgctgt tggtgctggg cagtctgtac
      421 ggattgatca atcgatcagc gcgcagttcg acgctgttag cacttggcta tctgatcatt
      481 ccgctcctgc tgttttacgc cgcgacattt actcggccgt tatacaccgg cccgcggcat
      541 ttgatcttga tcgcgccgcc gttttatctg ctcatggcct gcggcctcga cgcgatctgg
      601 aaacgatcgc gtcttatcgc ggtggctgca ctcctctgga gcgttgcgct ctcgggcatt
      661 tggttatatc ggcagtacat tgatccagcc gacaccaaag aagatattcg atcgatggcg
      721 caacttattt cggcgcgggt cacacctcaa gatgccgtga tcttggaagg cgcagtgggt
      781 tcattcacct tcgattacta ttacaccggt tcagcgccgc gttcattcat tccggttttt
      841 ccctcgtccg atgttgaggg cgcaattacc gcgctgaatg cacgcgcgca aaccgccgat
      901 cgagtgtggc tggtcactga tccgccaccg gagaatattt cttcaaacgc gctcgatcag
      961 tgggcgcgcg gtcacttgct gcgcttggat catcagcgct tcccggcgat gtggcttgga
     1021 tcagcctatc aactgtatac tgcacacttt ccgatttatg ctgctctgcc gatcgatgca
     1081 atgccgcgca atattcagtg gctgacggct gcattgcgtt tggccggtgc tggttcgatc
     1141 gttgtttcac ccgatcgagg tgttgtgacg acggcgctct actggcggat cgatgcttcg
     1201 cctacgagtg actttatatt tacacagcgc ctgatcgatg ataccggcgc ggagtggggt
     1261 ttgcagatcg gcgccgcgtt cgataactgg tcggccgcgc aatggccgat cggaaaactg
     1321 atcaaacaag aaacgcagat cgatctaccg cacggtttgc ccgccggtca ttatcgttta
     1381 ttggtgagcg ttgccgataa atcaggccag ccattcacct tgctgagtgg cttgggcgaa
     1441 atcgaagccg ctcaagtcga taattagcca tgactcaatc acgtatcacg catcacgcct
     1501 caagtttttt gagcgtcaaa acgatcgtga tcaccttcac ggtgatcgcg tcgatctaca
     1561 gcatcagcac gccgatcttc gaagcgtccg atgaattgtg gcattacccg ttggtacagt
     1621 ggctatcgaa aggcaatccg ctgccattac aagatgcaaa aaatgtcggg ccgtggaaac
     1681 aagaagcgag tcagccgcca ctgtattaca ccttaatggg ctgggcgact ttctggatcg
     1741 acacgcgtga catgccgcaa gtgcgcattg aaaatccgca tgtcgataac ggcgtcatca
     1801 cgaccgatgg caatatcaat cttgtcgtcc acaatcctga tcgagaatca ttcccgtggc
     1861 gcggcacggt gcttgccgtt cacctaattc gattcttgtc ggtgttgatg ggagcagtaa
     1921 cagtctggct gacttatcgt attgcacttg aattatttcc cgatcgatct tatctcgcct
     1981 taggcgcggc ggccgtcaat gccttcacgc cgatgttcgt cttcatcagc ggcgcgatca
     2041 acaatgacaa cttgactatg ctgttgtgtt cgttgtcgct gctgctcatg atcaagcgct
     2101 tgcgcgaaga acaaggggca ggcgggcgga aattttcatt tcgacttggc cgctggttgc
     2161 ccttaggtat cgtaattggc ttagcgacac tgaccaagac tagtgcatta ggtttgttgc
     2221 cgatcacggc gataacggtt gtcatcgttg catggcggcg gggatcgtgg cctgagtttt
     2281 ttgcgggcgc tatcgcaacg gccgtgccgg ttttattgat cgcaggttgg tggtatctgc
     2341 gcaacattca actctacggc gatgtgcttg gtctcagtgc cttcatcgat gtcctcggtc
     2401 gacgcgcagc tccagcgagt ttgctgcaat tgtggggtga acgctggggc tttatgctct
     2461 cgtattgggg cttgttcggc ggcgtaaatg tgccgcttga taattgggtg tatcacatct
     2521 tgaatgcctt ggcagttctc gcgttcatcg gcgcgatcat ctatcaggtg cgcatgacga
     2581 tggactggtt tcgctctgat cccattcata cgtggcgcga ctttagattt aaactgcgcg
     2641 attacctgca aggtcgtgcg ccgttattca tcgttggctt gtttggactc atcgtcgtcg
     2701 tctcgttgac gcagtgggca acgatcacgt ggtcgtcgca aggtcgatta gtcttttcgg
     2761 cgatctcgat gtggtcgatc ttcagcgtcg ttggactggc tactcttttc ggttctcggt
     2821 ttgcccgatt ggcgatcgcg gcgacttcgg tcctcatgtt cctgattact ttgctcgcgc
     2881 cctttaccac gatcacgcca gcgtatgctc gcagtctgcc agttcaatcc cttcagccgc
     2941 ccgccatttc ttttgatgtt actttcaatc atcaactcaa actgatcggc gctgatttca
     3001 aatcaacatc agctcaaccc ggcggatcta tcgagatcat attgtattgg caggccagcg
     3061 cgccgatcga taaaaactac agcaccttcg ttcatctgct agatgaaaat gagatcgttg
     3121 ttgctcaacg cgacatgttc cccggtcaag gtctgtggcc gacctcgcag atgaaggttg
     3181 gcgacatcat tccgagccgt tacgtgatca acctgccagc aacggcttac gcgcccgaca
     3241 aattgatctt cgaagttggc gtatacgatt acgcttctca aactagattg ttgatgctag
     3301 atggcgcgga caacttgcgt tttggatcga tcgatcttca atcggtagcg ggcgatgtcc
     3361 ctaacccgat cgattacaac tttggcgatc agatcgatct ggtcggctat tcactcgatc
     3421 gccgatcggc tgctccgaat gacgcgctgc agctcaccct gtactggcgc gcgcgatcga
     3481 aagtgccgat cgactacacc gtctttactc acctcctgca gccgccgcag acgatttggg
     3541 cgcagcagga caagccgctt agtccgccgt cgtcgagttg gacaatcggg caaacgatca
     3601 gccaaacgta tgatctccac ctcaaaccgg atgtgtcatc gggcgtgtat gagatcgaag
     3661 tgggcttata cgattccgcc aactttgatc gactgcggat cgttgccgac gatggccgta
     3721 tcacggataa ctttgtgctg ctcagcaaag tgcgcatcaa ataaatgaaa tcgctgctga
     3781 tgaagaattc gagattacgc gttaaaggga tactgcgatg aaggtttccc gtcaaaactt
     3841 cattccctgg tggacgaatt gggctgcacc cttcatgctg cttatttttg tcttagcgtc
     3901 cattttcttg gcgtttcggc agcacgcggc tttccagact aacttatatg atctcggtct
     3961 ttatgcgcag cgcgtctgga atctatcgcg tggggcgggg ttcactacct cgctcggact
     4021 gactaacttc ctgagcggtc atttcgcgcc gttgttggcg ttgatcgcgc cgacgtttct
     4081 tatttggccg gacgcgcgaa tgctgatgat aattcaggcg attgcgttga ccatgtcaat
     4141 cgtgcccggc tatttgattc tccgtatccg ctatccgttg atggcgcccg cactggttct
     4201 ggcgtttgtc ttcagcccgc tgctgcatca gacctccgcc acggaatttc atggcattat
     4261 attggctgcc ccgtttatgg cttgggcatt ctatgcgctg tatatgcggc gtactcgaat
     4321 gatgttggtc gcattagcct tcgcagtctt agcgcgcgaa gatatgggtt tgtatgtggc
     4381 ttgctttggt ttgttcatgc tgatctgccg caaagggcag cgattgattg gcctggcgtt
     4441 ggccgcgttc ggtggtgcct gggtgatcgt tgtaatcaat tgggtgatgc caatctttgg
     4501 ccacacttac cgtcacttta atgccttctc ggcgttaggc ggcgattcag tgactgagat
     4561 gatcgcgaac gtcattcatg atccgatccg tgtggcgagg attatcttca cctcagcgaa
     4621 gctggccgct ctggcaaaat ttattttgcc gctcgccggt ttaccgattg tcgcactggg
     4681 ttatcccttg ttgtggctgc cggtggtggc agtgtatttg ctctcgaatg ccacaggttc
     4741 aggcttattg aatagctggc gggttgcgcc gttcttaccc ttgttatggg gatcgatcgc
     4801 cgccttgttt gcacgactgc gtccacgttg ggcgattggc ggcatggcag tcttgatgct
     4861 agccacactc atcggttatc tgactttaag tccatttccg ggcggagggc agtttgatgc
     4921 gacactctat caagtgaatc agcacaccca gatcggcgaa cagattgtgg cgagcattcc
     4981 gcccgctgta tccgtcgcgg cacaggatgg attggcggca catctaagta cgcgtgcaca
     5041 gatgcggctc ttcccgttgt acagcctggc caatactccc gagttcattg tgttagatga
     5101 taaagcaact gatttctatc cacaaacagc cgatgaattt aaattggcat tactgaattt
     5161 gcagatgaat ccgcaatttg atgtagtgca cgaacaagac ggctactttg tatttcgatc
     5221 gactggcggc gtgaatcaat ccttgcagcc cacatcgttg acctattcgt cgacactcaa
     5281 gttatacggc ttcgatctcg cacaatccgc tggaagtaat ccgttcagtg ccgttagcgg
     5341 caatgcggat catggagata ttctacgcgt gtcgctgtac tggacagcgt tagtgccgat
     5401 gccgaatcat ctctcgattt cggttcgagt gaatgatgcg gctggaaata tcattgctca
     5461 agacgacagt tggccggcac gtggccaaat ccctacgccg ttgtgggaaa tcggtcgatc
     5521 gattcgtgac gttcactatc taaatttacc tgacagcgca ctgcaggagc tgctgacgct
     5581 caaggtgatc gtctatgcgg ccgatacgct gcaaccactt gagcccgtgg agggatatgt
     5641 tctttcagca ttactgggca cgcagccaaa atgattaatg ctgaaaatat ttattaagcg
     5701 tctgatgaac atccaattga tttttgtaac gtttatcggg ttggggctgt tgtgccccgt
     5761 tggcacacct atcttccagg cacaagataa atttatcttc gaagtgggtg
//
LOCUS       DASRTJ010000008         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_227361,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000008 DASRTJ010000000
VERSION     DASRTJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227361"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VFF70_00115"
     CDS             <1..1565
                     /locus_tag="VFF70_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43128.1"
                     /translation="SLITHHSTLLILFSLLPITLIATNIPLVNQHNVDADREAWGRYV
                     LSLPTPLRAALLVDSEKIAPLYYLQVTEHIRPDLDILVLGDESLYREELDKRIAAGQP
                     VYLARFLPNLPYHLRSLGPLVEVSMQPGTVAPNFQMNLHFGDAIDLTSISIDDNNPIR
                     VTFSWQAVTDARPNYHLRLQLIDDSGRVWWEDTGARPVNGYYPTGAWTKGETVTDYHE
                     IKPDPFVPPGRYTLRVGFYVPFRAEGLLTDDGTPWQVVTSVQVKSIDSPESLAHEIRS
                     TYGAMLELTAVDVLGIIPPLSHVVFRLNSIGADSVSNARLSMVNSSGLNISTAQQVIN
                     VGQSRLEFNAPEMNGVYTLRLDLQQPARCHWLAPITADCEIGSFEVSGEAIEHAINFE
                     NQVLLTEAKIDRATAKPGEAIHIDLTWRGLKTWSYDYTAFVHLIGPDGKVHGQIDQWP
                     VQGTLPTSNWVAGQTVNDPYVVTVTPDAPSGKYQVEAGWYLLATLRRLSVLDSSGRPS
                     DDHVIIGEFNVP"
     gene            1717..2748
                     /locus_tag="VFF70_00120"
     CDS             1717..2748
                     /locus_tag="VFF70_00120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43129.1"
                     /translation="MALEINTLTTTDLFKDLASEWNAILHNSAADTPFLTLEWQQTWW
                     DCLAEGELFVITVRDSGALIGIAPLFLVSKPFKDNEEPRRLLRLIGGVDASDYLDLIA
                     VRDREREVIDAMLSALAKSDRWDVLDLYNVPEASLARSVLPALAAPRQWKMIDEPQVV
                     CPIIPLPISFEEYLDSLDKKERHELRRKLRRAENAEGVTWYAVTAQDHEHDLTEAAES
                     FINLMMQSRSDKSDFMTAAMRRFFHSMIRAMHAGGFLHLSFLEVEGVKAATYLCFDYG
                     SRRLVFNSGLDTTQFQSLSAGIVLAARLIEESIQRGYTEFDFLRGNEDYKYRLGAKDT
                     YIYHVAIER"
BASE COUNT          658 a          721 c          769 g          706 t
ORIGIN      
        1 cctcactcat tactcatcac tcaacactcc tcattctctt ctctcttctg cccattactc
       61 tcatcgccac caacattccg ctcgtcaatc aacacaacgt cgatgcggat cgagaagcat
      121 gggggcgcta tgtcctctca ttgccaacac ctcttcgggc ggcgctgttg gtcgatagtg
      181 aaaagattgc gccgttatat tatctgcaag tcaccgaaca catccgcccc gatctggata
      241 ttttagttct gggcgatgaa tcgctttatc gagaagagtt ggataagcgc atcgcggccg
      301 gacaaccggt ctatctcgcg cggtttctgc caaacttgcc gtatcacctg cgatcacttg
      361 gcccgttagt ggaagtctcg atgcagccgg gcaccgttgc gccgaatttt caaatgaatc
      421 tacacttcgg cgacgcaatc gatctgacca gtatatcgat cgatgacaac aatccgattc
      481 gcgtcacctt cagctggcag gcagtgactg acgctcgacc gaactatcat ctgcgcttgc
      541 agttaattga cgattcaggt cgcgtctggt gggaagatac aggcgcgcgt ccggtcaatg
      601 gctattatcc gaccggcgcg tggacaaaag gcgagaccgt tactgattat cacgagatca
      661 aacccgatcc ctttgtgccg cccggtcgat acactttgcg tgttggcttc tacgtgccat
      721 ttcgtgccga aggtttgctg accgatgacg gcacgccatg gcaggtagtc acttcggttc
      781 aagtgaaatc gatcgattcg ccggaatcac tcgcacatga aattcgatca acatacggag
      841 ctatgttaga gttaaccgca gtcgatgtat tgggtattat ccctccgcta tcgcatgtag
      901 tgttccgcct gaattcgatc ggtgctgatt cagtttcgaa tgctagacta tcgatggtca
      961 acagcagtgg tttaaacata tcgacagccc agcaagtgat caatgtcgga cagtcgcgtc
     1021 tggaattcaa tgcgcctgaa atgaatggcg tctatacctt gcgacttgat cttcaacaac
     1081 ccgctcgctg tcattggctt gcaccgatca ctgccgattg cgaaatcggc tcgttcgagg
     1141 tctccggcga agcgatcgag catgcgatca actttgagaa tcaagtgttg ctaaccgagg
     1201 cgaagattga tcgggctaca gcaaagccgg gtgaagccat ccacatcgat ctcacatggc
     1261 gcggattgaa aacatggtcg tacgattaca cggcgttcgt gcatttgatc ggaccggatg
     1321 gcaaagtgca tgggcagatc gatcagtggc cggtgcaagg cacgctgccc acgtcgaatt
     1381 gggtggcggg acagacagtg aatgatccgt atgttgtgac agtcacgccc gatgctccaa
     1441 gcggcaagta tcaagtggaa gcaggttggt atttgctggc aactttgcgc cgactatcgg
     1501 tgttggattc aagtggacgg cctagcgatg atcatgtaat cattggcgaa ttcaatgtgc
     1561 cttgagcctt cgctcagatt cgatcttttt cgaatggggg ggagggtcaa aaatgccgac
     1621 cgcagttctc ggtaatcggg cggagcaatt agcggctctc aacctcatcg ggtataatct
     1681 tcgagcgttt agcgctgaaa attggaattg gatcaattgg cactagaaat taacacctta
     1741 accacaacgg atttattcaa agacttggct tcagagtgga atgcgatact gcacaacagc
     1801 gcggccgata cgcccttcct gacgctcgaa tggcagcaga cgtggtggga ctgtttggct
     1861 gagggcgaat tgtttgtcat caccgtccgc gacagcggcg cactcattgg gattgcgccg
     1921 ttgtttcttg tatcgaaacc gttcaaagat aatgaagaac ccagacgctt gctgcgcttg
     1981 atcggcggcg tggatgcgtc cgattatctg gacttgatcg cggtgcgtga tcgcgaacgt
     2041 gaagtgatcg atgcgatgct gtctgcgctg gcaaaatccg atcgatggga tgtgctcgat
     2101 ctgtataacg tgccggaagc ctcgctagcc cgatcggtat taccggcgtt agcggcgccg
     2161 cgccaatgga aaatgatcga cgaaccgcag gtcgtctgcc cgatcatccc tttgccgatc
     2221 tcgtttgaag aatacttgga cagcctcgat aaaaaggagc gccatgaact gcggcgcaaa
     2281 ctgcggcgcg cagaaaacgc tgaaggcgtg acgtggtacg cggtcacggc acaagatcac
     2341 gaacacgatc tgactgaagc ggctgagtcc ttcatcaact tgatgatgca gagccgatcg
     2401 gacaagtcgg acttcatgac ggcggcgatg cggcgcttct ttcacagcat gatcagagcc
     2461 atgcatgccg gcggcttctt gcatttgtca ttcttggaag tggagggcgt caaagctgcc
     2521 acgtatttgt gcttcgatta tggttcgcgc cgtttggttt ttaactccgg gctggacacc
     2581 acgcagtttc aatctttgag cgcgggcatc gtgctggccg cgcggctgat cgaagaatcg
     2641 atccagcgag gctacaccga gttcgatttt ctgcgcggca atgaagatta caaatatcgc
     2701 ttgggcgcta aagatacata catttatcac gtggcgatcg aacgatgatt gcgatcatct
     2761 cgcggataga atagaaaact gatattgtta tatttgtttt tgtccgtgtt tcctttaatc
     2821 tgtgaggaaa aggttttagg ttgtcaccct gagc
//
LOCUS       DASRTJ010000009         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_76098,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000009 DASRTJ010000000
VERSION     DASRTJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76098"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VFF70_00125"
     CDS             complement(<1..1946)
                     /locus_tag="VFF70_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY43130.1"
                     /translation="MDVTGRIGQYTIIQSIGQGGMASVYKAYQPNLDRYVAIKVLPAQ
                     HALTPGFAERFDREAKAVALLNHPNILPIIDYGQEGDLTYIVMKYVPHGTLADRTGKP
                     IELALATGLIEQIAAALDHAHSRGIIHRDIKPSNVLLDEGDWVQLADFGLARVLSGPS
                     ELTASGASMGSPAYISPEQGQGLPLDHHTDIYSLGVVLYEMATGQLPFSAETAMTLVF
                     KHIYEEPPTPRSINPALSSAIEAVILKAMSKTTVDRYHSAGDLARELRLAVSGSADSS
                     ASGVLDRPALRHAVGQVSSDPIAPISSVSAINLDEQAVELMIETIPTVSHFIGREKEL
                     ANYRARLQRDGFIVISGLAGAGKTALGAKLAHEVAKDAKHIFWFTFDQIEQSTVDALL
                     WALGAFFESHGDHNLWRYLNNEPESQKPLDRTVRMNLLLAGLTANRYVLCFDDLQIVE
                     RVPEIAYVFKLIRQRLAEQRESTASQVILLGRQVSRELESFVTAPLSGFDYEEAVAFV
                     MEQPVHLPAAVVRRLWERTEGNPKLLELSVSAIEPLVGESAAVDRFVEAMSRKGDVRD
                     YLMSNIYGALRDDEKVIMGALSVFPNSIERDVAQTILEADGIDDVVTRLDALIDRQLL
                     DETDQDQIHCHGLVRDYCYHVLSRA"
BASE COUNT          500 a          628 c          567 g          479 t
ORIGIN      
        1 gcgcggctga gaacgtgata acaataatcg cgcaccaagc cgtgacaatg gatctgatcc
       61 tgatcggtct catccaacag ctgccgatcg attagagcgt cgaggcgcgt caccacgtca
      121 tcgatgccgt cggcttccag aatcgtctgg gccacgtcgc gctcgatcga gttgggaaag
      181 accgacagtg cgcccatgat tactttttcg tcgtcgcgca gcgcgccgta aatattcgac
      241 atgagataat cacgcacgtc gcctttgcgc gacatcgcct cgacgaagcg atcaacagcg
      301 gcagactcac ccacgagcgg ctcgatcgcc gatacactca attccaacaa cttggggttg
      361 ccttcggtgc gttcccacaa gcggcgcacg accgcagccg gcaaatgaac aggctgctcc
      421 atgacaaacg cgaccgcttc ctcataatcg aaaccactta acggcgcagt aacaaatgac
      481 tccaactcac gcgatacttg acgtccgagc aaaattactt gcgaggctgt gctctcgcgc
      541 tgctcagcca aacgctggcg gatcagtttg aagacgtacg cgatctccgg cacacgctcg
      601 acgatctgta gatcgtcgaa gcacaacacg taacggttgg cggtcaagcc tgccaacagc
      661 agattcatac gcaccgtgcg atcgagcggc ttctgcgatt ctggttcgtt attgaggtac
      721 cgccacaaat tgtgatcgcc gtggctttcg aaaaacgcgc ccagcgccca caatagcgcg
      781 tcaaccgttg actgctctat ctgatcgaac gtgaaccaaa aaatatgttt ggcgtctttt
      841 gccacttcat gcgcgagctt ggcgcccaaa gcggttttgc ccgcgcccgc cagaccggag
      901 attacaataa agccatcccg ctgcaagcgc gcccggtaat tcgctaattc tttttcacgt
      961 ccgataaaat gcgaaactgt agggattgtt tcgatcatca actccacggc ctgctcgtcg
     1021 agattgatcg cagacacaga actgatcggc gcgatcgggt cagaagacac ttgccccact
     1081 gcgtggcgca gtgcgggtcg atcgagtacg cctgacgcgc tagaatcggc cgaaccggaa
     1141 accgccaaac ggagctcgcg tgccaaatcg cccgcggagt gatatcgatc caccgtcgtc
     1201 ttggacattg ctttcaaaat cacggcttcg atcgcagatg acaatgcggg gttgatcgaa
     1261 cgcggcgtgg gcggttcttc atagatgtgc ttaaagacca gcgtcatggc agtctcagcg
     1321 ctgaacggca gttggcctgt cgccatttca tacagcacca cgcctaacga atagatgtcc
     1381 gtgtgatgat cgagcggcag accctgacct tgttcgggcg agatgtatgc cggcgaaccc
     1441 atacttgcgc cggacgcagt aagttcgctc ggtccagaca acactcgcgc taaaccaaaa
     1501 tccgctagct gtacccaatc gccttcgtct aacaatacgt tcgagggttt gatgtcgcga
     1561 tggatgatgc cgcgactatg ggcatgatca agcgcagccg caatctgctc gatcaatccg
     1621 gtggccagcg ccagctcgat tggtttaccc gtccgatcgg ccagcgtgcc atgcggcaca
     1681 tatttcatga cgatgtatgt cagatcgcct tcttgtccgt aatcgatgat cggtagaata
     1741 ttggggtggt tgagcagcgc gacggctttg gcttcgcgat cgaagcgttc ggcgaagccc
     1801 ggcgtgaggg cgtgctgcgc agggagaacc ttgattgcga catagcgatc gagattgggc
     1861 tggtaggctt tataaacgga tgccatgcct ccttgcccga ttgattgaat gatcgtatat
     1921 tgcccgatcc tgccagttac gtccatgcgc tcctcgaaat gcattgtaca acaattctaa
     1981 cttcttatac aaaaagttgg atcgtcattg cgaggcggtc tcaggccgac gaagcaatcc
     2041 ctcctctatg ttttccgaaa ttacttctcc gcatcggtcc aaagtatgaa agcgcgtgca
     2101 tcatcgctta atgctttccc tgcaaaggcg gatagtcagt cgactcattt ctgcacccgc
     2161 ttcaacaact tctc
//
LOCUS       DASRTJ010000010         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_189417,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000010 DASRTJ010000000
VERSION     DASRTJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189417"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFF70_00130"
     CDS             <1..895
                     /locus_tag="VFF70_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43131.1"
                     /translation="FRSIIRPIDQLTLAAHALTRGDLSTRVEVDDRPTRLGTDELSDL
                     GSAFNSMADHLQQAEQLRRDLTADIAHELRTPLSVIRANLEAMIDGVYPTDGVHIHTT
                     LDQVNLLARLIEDLRTLALAEAGQLPLVKRPADVTALISSALASFETQAQAQQIDLTS
                     NVDTNLPMIAIDPDRIQQALAILIANALRHTPVNGSIKVSGHQAGKQLIIEVTDTGSG
                     IAPEDLPHVFERFYRADKHLHRAARGSAQEQVPRSRAGGGSGLGLAIAKSIVGAHAGT
                     IETHSELGKGTTMRLQLPIEL"
     gene            934..1134
                     /locus_tag="VFF70_00135"
     CDS             934..1134
                     /locus_tag="VFF70_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZY43132.1"
                     /translation="MSADNYIKLDSFLKWQNLVETGGQAKVVIQNGEVKVNGKNETRR
                     GRKLIDGDKVTYNGKTIVVKLV"
     gene            complement(1165..2202)
                     /locus_tag="VFF70_00140"
     CDS             complement(1165..2202)
                     /locus_tag="VFF70_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43133.1"
                     /translation="MENKLPYSPSWIDRFTRWFDRVPGPKWLYYMIAAIVLALVHAII
                     NWQSGAYPAGTFDAFHVWFVVEIVMTVAFVHYLDRYSDAALERFRPALQISDDAYADL
                     RYQLTHLPPRRTLVISLLMGVLFAGVVAAAPSEMMRQFNFDSTPTLKAINVGLFAVTW
                     FLFGTLYYHAFHQLRLISRIYAEHAQIDLLSLLPLYAFSGVTARTAIGITIMNVMWTI
                     TEPQTLSANLTSAVPYLIMALIIFVWPLWGLHRRILIEKRRAIDANGQHWQAITAGLH
                     RDAEAKMIDSATKYRDFLISLDMERSLLKKVPTWPWEPETLRGFLSALALPIVLFLIQ
                     YVIGQMLSGGK"
     gene            complement(2353..>4082)
                     /locus_tag="VFF70_00145"
     CDS             complement(2353..>4082)
                     /locus_tag="VFF70_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZY43134.1"
                     /translation="SQYTPGVGWAQAITYRSRVLKEKEWAGSILVALGGDASTAANGF
                     WASLNIVTTQSLPYLYLIEDNSYGISVPSTYQTPGGDLAKNLSSFTGLYLIDGDGTDP
                     IDAAIKIDQAVQYVRSHRGPALLRLSVPRLSGHSFTDNQSYKTLQQIETEQARDPLPK
                     LKQFILNQKYLSEAEWAALEQSVEAEVAAARDRALAQAEPDTTSTQRHVFFDGSNPQT
                     IGGLIGDGIKLPEGSTTPNVTDPGSRLNLIDAIKRTLEVELSINPRLAIFGEDVGVKG
                     GVHGATTDLQLKFGADRVFDTSLSEDGIIGRSVGLALAGLMPVPEIQFRKYTDPATEQ
                     INNVGTMRWRTMSKFAAPIVVRMPVGFGRKTGDPWHSVSGETIHAHTIGWRVAYPSNA
                     EDAVGLLRSALRGNDPTFFYEHRALYDTAPARRPYPGDEYVLPFGVANVLSSGDDLTV
                     VTWGSMVQRCVDAANPPGGFKPPGGSGGSIEIIDLRTIVPWDKAAVLKSVSKTGKCLI
                     VHEDGWTVGFGAEIAATIAQEIFFDLDAPIVRLATPDVPLPYNTGLMDSVVPTVEKIR
                     EEMERLLRI"
BASE COUNT          966 a         1153 c         1077 g          886 t
ORIGIN      
        1 tttccgcagc atcatccgcc cgatcgatca actcaccctc gctgcccacg ccttgacgcg
       61 cggcgatcta tcgactcgcg tcgaagtaga tgatcgtccg acgcgcctgg gcacggatga
      121 actgagcgat ctcggctcgg ctttcaacag catggctgat catttgcagc aggccgaaca
      181 actccgccgc gatctgaccg ccgacatcgc gcacgaactg cgcacgccgc tgagtgtgat
      241 ccgcgccaat ctcgaagcga tgatcgatgg ggtctatccc actgacggcg ttcacattca
      301 cactacgctc gatcaagtca atctccttgc acgcttgatc gaagacctgc gcacgttggc
      361 tttagccgaa gccgggcaac tgcccttagt caaacggccg gccgatgtca ccgccttgat
      421 ctcaagcgcg ctcgcttcat tcgagacaca agcgcaagcc cagcagatcg atctaacatc
      481 caacgtggac acaaatctcc ccatgatcgc gatcgatccc gatcggattc agcaagcgct
      541 cgcgatcttg atcgcgaatg cgctgcgcca cacccccgtc aacggatcga tcaaagtatc
      601 cggtcatcaa gccggcaagc aattgatcat tgaagtcacc gataccggtt cgggcatcgc
      661 gccagaagat ctgccgcacg tctttgagcg cttctatcgc gccgacaaac acttgcaccg
      721 cgctgcgcgc ggtagcgcgc aagagcaggt gcctcgatcg cgcgcgggcg gcggttcagg
      781 gttggggctg gcgattgcca agagcattgt tggcgcgcat gctggaacta tcgaaacgca
      841 cagcgagttg ggcaaaggca cgacaatgag gcttcaatta ccgatcgaat tgtagagatg
      901 ttccagtggg tcgtctttac gtgaggggct aatatgagcg cggataacta catcaaactc
      961 gattcattcc tcaagtggca gaatcttgtc gagaccggcg gccaggccaa ggtcgtcatt
     1021 caaaacggcg aggtcaaagt caacgggaag aatgaaacgc gccgaggccg aaaattgatc
     1081 gatggggata aagtgactta caatggtaag acgatcgttg taaagttagt ataggggcga
     1141 gcacatcggc tcgccccact tttttcattt gccgccgctc aacatttgcc cgatcacata
     1201 ctgaatcaag aagagcacaa tcggcagcgc cagcgcagat aagaaaccgc gcagcgtttc
     1261 cggttcccac ggccacgtcg gcactttctt caagaggctg cgttccatat ccaaacttat
     1321 cagaaagtcc cgatatttgg tggcgctatc gatcatcttc gcctcggcat cgcggtgcag
     1381 ccccgcggtg atcgcttgcc agtgttggcc gttggcatcg atcgcgcgcc gtttctcgat
     1441 caagatgcgg cgatgaagac cccacaacgg ccacacgaaa ataatcagcg ccatgatcag
     1501 atagggcacg gcactcgtca ggttggcgct gagagtttga ggttcggtga tcgtccacat
     1561 cacattcatg atggtgatgc cgatggcggt gcgcgccgtc acgcccgaaa aggcatacaa
     1621 cggtaacagg ctgagcaaat cgatctgcgc gtgttcggcg tagatgcggc tgattaagcg
     1681 cagttgatga aaggcatgat agtacaacgt gccaaataga aaccacgtca cggcgaagag
     1741 gcccacattg atcgccttta aggtgggcgt cgagtcaaaa ttgaattgcc tcatcatctc
     1801 cgacggggcc gcggctacaa cgcctgcgaa cagcacaccc atgagcaggc taatcaccaa
     1861 tgttcgccgt ggtggcagat gcgtcaactg ataacgcaaa tccgcatacg catcgtcact
     1921 gatctgcaac gccggccgga atcgttccag cgcagcgtct gaatagcgat cgagataatg
     1981 gacgaacgct acagtcatca caatctcgac cacgaaccag acatgaaatg catcaaacgt
     2041 gcccgccgga taggcgcccg attgccaatt gataatcgca tgaaccagcg cgagtacgat
     2101 tgcggcgatc atgtaataca accatttcgg gccgggtact cgatcgaacc agcgcgtaaa
     2161 ccgatcgatc caactgggcg aatagggcag tttattttcc atgttgatat cctctttgcg
     2221 tgttctcaaa tatagttaag gtttgtcatt tcgaacgaag tgagaaatct ctacctctgt
     2281 aaacaaacat tgctaattgt cgcaaagatt tctcaccgct gcgcggttcg aaatgacatg
     2341 cttcaacaaa cctcaaattc tcaacaacct ttccatctct tcacgaatct tctcgaccgt
     2401 cggcacgact gaatccatca agcccgtatt atacggcaat ggcacatccg gcgtggccag
     2461 tcgtacgatc ggcgcatcga gatcaaaaaa tatttcttgc gcgatcgtcg cggcgatctc
     2521 ggcgccgaag ccaaccgtcc agccatcttc gtgaacgatc agacatttgc ccgtttttga
     2581 aactgatttc aatactgccg ctttgtccca cggcacaatt gtacgtagat cgatgatctc
     2641 gatcgatccg cccgatcctc ccggaggttt gaaacctccg ggaggatttg ctgcatcgac
     2701 acaccgttgc accatcgaac cccaagtcac aacggtgaga tcatcgccgc tcgataaaac
     2761 attggcgacg ccgaagggca gcacgtactc atcgccggga tacggtcgac gcgcaggcgc
     2821 agtgtcatac aacgcccgat gctcgtagaa aaatgttgga tcattgccgc gcaacgcgga
     2881 tcgcaataag ccgacggcgt cttcggcatt tgacggatag gcgacgcgcc agccgatggt
     2941 gtgcgcgtga atcgtttcgc cgctgacgct gtgccacgga tcgccggtct tgcgtccgaa
     3001 gccgacgggc atacgcacga cgatcggcgc ggcaaatttg ctcatggtgc gccagcgcat
     3061 cgtgcccaca ttgttgattt gttctgtcgc gggatcagta tactttctga attgaatttc
     3121 aggcacaggc atcaaacccg ctaaagccag accaaccgat cgaccgatga tgccatcttc
     3181 gcttaacgat gtatcgaata cgcgatccgc gccgaacttt aattgcaagt cggtcgtcgc
     3241 gccatgcacg ccgcccttca cgcctacatc ttcgccgaag atcgccaaac gcggattgat
     3301 cgacaactca acttccaagg tgcgtttgat cgcatcgatc aggttcaagc ggctgccagg
     3361 atcggttaca ttcggcgtag tcgatccttc cggcaatttt attccatcgc cgattagtcc
     3421 gccgatcgtt tgcggattcg atccgtcgaa gaagacatgc cgctgggtcg atgtagtatc
     3481 aggttcggct tgtgctaacg cccgatcgcg tgcagcggcg acctcggcct ccaccgattg
     3541 ttccagcgct gcccattcgg cttcgcttaa atatttttga ttcaaaataa attgttttag
     3601 cttcggcaat ggatcgcgcg cttgttccgt ttcgatctgc tgcagagttt tgtacgattg
     3661 attatcggtg aaggagtggc cgcttagacg cggcacagac aatcggagta aagccggacc
     3721 gcgatgggat cgaacgtact gcacggcttg atcgatcttg atcgccgcat cgatcggatc
     3781 agtcccatcg ccatcgatca gatataagcc ggtgaaggaa gataaatttt tggcaagatc
     3841 gccgccgggg gtttgataag tgctgggtac ggagatgccg taactgttgt cttcaatcaa
     3901 atagaggtaa ggcagtgatt gcgtggtgac gatattgagc gaggcccaga agccattggc
     3961 cgcagttgaa gcatcgccgc ctaaagcgac gaggattgat ccagcccact ctttttcttt
     4021 cagcacgcgc gatcgatagg tgatggcttg cgcccaaccg acgccaggcg tgtattgcga
     4081 gc
//
LOCUS       DASRTJ010000011         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_38264,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000011 DASRTJ010000000
VERSION     DASRTJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38264"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFF70_00150"
     CDS             complement(<1..427)
                     /locus_tag="VFF70_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43135.1"
                     /translation="MDAIQLENVSKVYHIGKVETHALEDINLSIGEGEFTALVGPSGS
                     GKTTLLQLIGCLDKPDHGAVKIKGQDVTRFNANQRADLRRESIGFIFQFFALVPVLTA
                     YENVELPLLLGNVKTKERRERVMELLKAVGLSDRTKHRPD"
     gene            complement(548..>1511)
                     /locus_tag="VFF70_00155"
     CDS             complement(548..>1511)
                     /locus_tag="VFF70_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZY43136.1"
                     /translation="ASALSEVKAAVPVLWAGGIVNTIEDSAGVQIFGIDTTSPIYEPI
                     QSSIVAGNFLTADDRNGILLGKRLAASLGVAVGQNINVAIINSDGQPDQQSFTIRGLY
                     STGIPSYDQNAVFLPLSKAQAFAGANGKASAIFIMLKDEADTDKVAAALRGPEVSVVT
                     WSELNKVFVQTMQLASSFYVLLDGIVILIVAVIIANTLLMAVFERTREMGILAALGMK
                     RRQIMQMFLLEAASLGLAGVIIGVILGSAGVAYMTVNGIPLTEDVAAVAGNSFALGTS
                     MNARFDVASFAALSIATLIVVILASLYPARYASRLEPVEALRAD"
BASE COUNT          357 a          437 c          390 g          327 t
ORIGIN      
        1 gatcgggccg gtgcttagtt cgatcggata agcccacagc cttcaacaat tccatcacgc
       61 gctcgcgccg ctctttggtt ttgacgttgc ccaacaacag cggcagttcg acattttcat
      121 acgcggtcag caccggcacg agcgcgaaga attgaaagat gaaaccgatg ctctcgcggc
      181 gcagatcggc gcgctgattg gcgttgaagc gcgtgacgtc ctgccccttg attttcaccg
      241 cgccgtgatc gggtttgtcg agacacccga ttaattgcag cagcgtcgtc ttgcctgaac
      301 ctgacggtcc gaccagcgca gtgaactcgc cttcgccaat actcaggttg atgtcctcca
      361 gcgcatgcgt ttcgaccttg ccgatatgat agaccttact gacattttct aattgaatag
      421 catccatgat tattttcctt ttgagttgtc cgccgtcatt gcgagcgaag cgaagcaatc
      481 tccacgataa tcaactgaga ttgcttcggc gcaaaaagat gcgcctcgca atgacgttag
      541 agtttagtta atccgcccgc agcgcttcga ccggctccaa acggctggca tagcgcgcag
      601 gatagagcga agccaagatc acaacgatca aagtcgcgat cgatagcgcg gcgaaagagg
      661 cgacatcgaa acgggcattc atgctcgtgc ctaaggcgaa gctattgccc gcgaccgccg
      721 cgacatcttc ggtgagcggg ataccgttga cggtcatgta agccacacca gccgaaccca
      781 agatcacacc gatgatgaca ccggccaagc ctaaactcgc ggcttccaac aaaaacatct
      841 gcatgatctg tcgccgcttc atgccgagtg cggccagaat acccatttca cgcgtgcgct
      901 cgaagacggc catcaacaac gtgttggcga tgatcaccgc cacgatcaag attacgatcc
      961 catccaacag cacatagaag ctggaggcca gctgcattgt ttggacaaac actttgttca
     1021 attccgacca cgtcacgaca ctgacctcgg ggccgcgcaa agcggcagca actttatcgg
     1081 tgtcagcctc atccttgagc atgatgaaga tagcgctggc tttgccattc gcgccggcaa
     1141 aggcttgcgc tttggacagc ggcagaaaga ccgcgttctg atcgtaactg ggaatgcccg
     1201 tggaatacag gcctcgtatg gtgaatgatt gttgatcagg ctgaccgtcc gaattgatga
     1261 tcgccacatt gatattttgt ccgacggcga cgcctaagct agcggccaat cgtttgccga
     1321 gcaaaatccc gtttcgatcg tccgccgtta gaaaatttcc ggcgacgatc gacgactgga
     1381 tcggttcata aatcggcgaa gtagtgtcga taccgaaaat ttggacgccg gctgaatcct
     1441 caatcgtgtt tacaatccca ccggcccaca gcacgggcac ggccgctttc acttcgctca
     1501 acgcgctggc t
//
LOCUS       DASRTJ010000012         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_227576,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000012 DASRTJ010000000
VERSION     DASRTJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227576"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="VFF70_00160"
     CDS             complement(<1..109)
                     /locus_tag="VFF70_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZY43137.1"
                     /translation="MEKHSTAQDVAKLAGVSRTTVSFVLNKVDGIRISPE"
     gene            complement(397..810)
                     /locus_tag="VFF70_00165"
     CDS             complement(397..810)
                     /locus_tag="VFF70_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZY43138.1"
                     /translation="MEISIDEVQGRVPVTILVLHGDLDGSNYQTLITTADKVYQSGTT
                     NLILDLSQLNYMSSAGLVALHAIAKLLQGGPLPDPNAGWEAFHAIERDQAAGTHSHLK
                     LLNPQGKVDQVLVMTGMKSFFDVYTDRQAAIAACG"
     gene            complement(794..1216)
                     /locus_tag="VFF70_00170"
     CDS             complement(794..1216)
                     /locus_tag="VFF70_00170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43139.1"
                     /translation="MEQLIVARQFVEDTAARLAVRPDAISKIVLAVDELITNIIVHGY
                     QGRPGSIDIELQAIGSDLEIRLRDQSPPFDPATVPEPAFDMPLDERPLGGLGIYLARK
                     IMDSLTYQSTVGRGNELTLLKKQVIHTDSKEIGNGNQH"
     gene            complement(1422..2282)
                     /locus_tag="VFF70_00175"
     CDS             complement(1422..2282)
                     /locus_tag="VFF70_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZY43140.1"
                     /translation="MKLATYQLNNKISIGAVRDNSIVDLSSIAPDMLSFIAAGSSALD
                     RARELTRSARQAISIDSVKLLAPIPNPRRNIMCLGQNYEEHARERARSAGKEYVPLKY
                     PTFFTKATHTINGPYDDIPFDASVSKHIDWEAELGVILGRSGKNIPLDRAMDYVFGYA
                     CINDVTARDLQAQHTQFFKGKSLDGSCPLGPWIVTADEIADPHALKIMSRVNGVTKQD
                     STTGEMIFKIPQLISILSCGMTLDAGDIISTGTPSGVGEARTPPEFLKPGDIVEVEVE
                     RIGLLRNRIS"
     gene            complement(2347..2422)
                     /locus_tag="VFF70_00180"
     tRNA            complement(2347..2422)
                     /locus_tag="VFF70_00180"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2387..2389),aa:Gly,seq:tcc)
     gene            complement(2427..2499)
                     /locus_tag="VFF70_00185"
     tRNA            complement(2427..2499)
                     /locus_tag="VFF70_00185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2464..2466),aa:Pro,seq:tgg)
     gene            complement(2549..2734)
                     /locus_tag="VFF70_00190"
                     /pseudo
     CDS             complement(2549..2734)
                     /locus_tag="VFF70_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2777..>3048)
                     /locus_tag="VFF70_00195"
     CDS             complement(2777..>3048)
                     /locus_tag="VFF70_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY43141.1"
                     /translation="IEIGAEFAALAKAHGKTPGQLALAWCKDQPGITSPIVGPRTIEQ
                     LYDLLPAMEMSLSAEERSACDDLNPPGGVIANFHNTSGWLKTPIG"
BASE COUNT          707 a          821 c          792 g          728 t
ORIGIN      
        1 tctcagggct gatgcgtatc ccgtcaactt tgtttaggac aaaggaaacc gttgtgcggg
       61 acacaccagc cagtttggct acatcttgag cagtcgagtg tttttccatg ctaacacgtt
      121 ttactaacac gagttagcaa gagtatatta taaaagcctg gtaaaagtca ataccgaaaa
      181 ttcaggaaga tcaatgagga taaggcgagg caattttgtg ttgcggcgat ggaagcgagt
      241 gcttcatgat gcgggggcag atcgcggagg attgatcatt tctcatggaa tagggcacga
      301 cggcgcggat aggcgatcta gccgattagt aaacgcacca ccgatcagca ccgccccaga
      361 cgatgcttga ttctcacgtt gattcgatct cctgatttac ccacacgccg cgatggcagc
      421 ctgccgatcc gtgtagacat caaagaagct tttcatgccg gtcatgacca acacttgatc
      481 gaccttgccc tgcgggttca atagtttaag gtgggagtgt gtgcccgccg cttgatcgcg
      541 ctcgatggcg tggaacgcct cccaaccggc attcggatcg ggcagcggcc cgccctgcag
      601 caatttcgca atcgcatgca gcgcaaccaa accggcactg ctcatataat tcaactgact
      661 taaatcaagt atcaggttgg ttgtaccaga ttgataaact ttatcggctg ttgtgatcaa
      721 agtttgataa ttagaaccgt ccaaatcacc atggagaacg aggattgtca ccgggactct
      781 accctgaacc tcatcaatgc tgatttccat tgcctatctc ctttgagtca gtgtggataa
      841 cttgcttttt caacaaagtc aattcattgc cccgcccaac tgtggattga taagtgagtg
      901 agtccatgat ctttcgagcg agatagatac ctagcccgcc cagtggtcgc tcgtccaaag
      961 gcatatcgaa cgccggctcg ggcacggtgg ccggatcgaa tggcggtgat tgatcacgca
     1021 accgaatttc aagatcagaa ccgatcgctt gcagttcaat gtcaatgctg cccggccggc
     1081 cttgatagcc atgcacgatg atgttggtaa tcaactcgtc tacggccagt acaattttgg
     1141 aaatcgcatc tggtctaacc gctagccgtg cagctgtatc ctcgacgaac tgtcgagcga
     1201 cgattaattg ttccacatca gccgcgattc gaaattgata caccattcaa gggtctaccg
     1261 atctgatcgc tcacaccaaa ctcgatcgag tcaacccacc tctcctcagt actgggtgaa
     1321 ccacaaaaaa tcaataaggt cgattataga tcatttttcg cgagtgtgtt cgtaccacac
     1381 gcttaggcat cgccgatgaa ttagcagctc atttagcggc tttagctgat ccgattacgc
     1441 agcaacccga tccgttccac ttctacttcg acaatatcgc ccggtttcag aaattctggc
     1501 ggcgtgcgcg cttcgccgac cccgctgggt gtgccggtcg atatgatgtc gccggcatcg
     1561 agcgtcatac cgcacgataa gatcgagatg agctgcggaa ttttgaaaat catttcgccc
     1621 gtcgtcgagt cttgtttggt cacgccgttt acgcgagaca tgatcttcaa tgcatgcgga
     1681 tccgctattt catcggccgt tacgatccac ggtcccagcg ggcacgaacc gtcaaggctc
     1741 ttgcctttga agaactgcgt gtgctgcgct tgtagatcac gcgcggtcac atcattgatg
     1801 caggcgtaac cgaatacgta gtccatcgct cgatcgagcg gaatgttctt gcccgatcga
     1861 ccgaggatca cacccagttc ggcttcccaa tcgatgtgct ttgagaccga agcatcaaat
     1921 gggatgtcgt catacggccc gttaatggta tgagtagcct tagtaaaaaa cgtgggatat
     1981 ttcagcggca catattcttt gccggccgat cgagcgcgtt cgcgcgcatg ctcttcgtag
     2041 ttctgtccca agcacataat gttgcggcgc ggattcggaa tcggcgcgag gagtttcacc
     2101 gaatcgatcg agatggcctg cctggccgat cgggtcagct cccgcgcccg atcgagcgcc
     2161 gatgatcccg ctgcgatgaa gctcagcatg tccggagcaa tcgacgacag atcgacgatc
     2221 gagttatcgc gcactgcgcc aatgctaatt ttattattta gttgataggt ggcgagtttc
     2281 aaattgcctc cgattgattg atgtaacaaa aacgatcgat tgcacgagac aatcgatcgt
     2341 actggttgga gcgggagacg ggactcggac ccgcgacatt ctgcttggaa ggcagacgct
     2401 ctaccaactg agctactccc gcgcagtcgg ggtggccgga cttgaaccgg cgaccccacg
     2461 gtcccaaacc gtgtgcgcta ccaactgcgc tacaccccga agcgattcga attgtaccct
     2521 gtcgatccgc ttcggtcaag gattcttatt attcgctgcc cgcgtcttgc gtcagccggc
     2581 tggcgatcaa gatcggaatt gccgtggcta agatcacgaa cacagccgcg acattggtga
     2641 ccggccgctg atgcgaacga ttaagttggc tgaaaatcca aatgggcagc gtctcttgct
     2701 gaccggccgt gaaggtggta acgatgatct catcctcgac ttcgttacga gagtctttga
     2761 aatgtgcatc gccaatctaa ccgatcggcg tcttcagcca gccactggta ttgtggaagt
     2821 tggcgatcac accaccaggc ggattgagat catcgcatgc ggatcgttct tcagcgctta
     2881 acgacatctc cattgcaggc agcaggtcgt atagctgctc gatcgtccgc ggtccgacga
     2941 tcggtgaggt aataccgggt tgatctttgc accacgccag agcgagttgg cccggggtct
     3001 tgccatgcgc tttcgccaat gcggcaaact ccgcgccgat ctcgatcc
//
LOCUS       DASRTJ010000013         5292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_554,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000013 DASRTJ010000000
VERSION     DASRTJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFF70_00200"
     CDS             complement(<1..349)
                     /locus_tag="VFF70_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43142.1"
                     /translation="MNQWSLFKRSWSIFWKNRTLWVFGLLAALGGGAASINFRGGDTS
                     QFANLPLGGREMLRQMFQSIDVNALIGIGVVIGLVTFLIGTFGRAALYKMINAIEENQ
                     PLSVGAGFTAAGKK"
     gene            complement(356..1189)
                     /locus_tag="VFF70_00205"
     CDS             complement(356..1189)
                     /locus_tag="VFF70_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43143.1"
                     /translation="MNRTALLKRAGSIWLHTPALWIVTLIGILVQAIASYALREPSIG
                     VGILQTVLAFIVTAFTSGALISLVNSAASEERASIGAGIGSGLRNLAPLLITSLILAI
                     PVWILLVLATGSITDVIASGLGQPNSLQISDLLNNLGGLLSIVGVVFVIAVLVHAISV
                     GAERAIVIDGQAIVAALKKSVLLLRTKVVDFITWGLILAVAIIGLEMIVAVIFGQLIN
                     VPDFDQSVLGTILITLNVIVGALTTVLISTIWTLVYREWQNPAVVEAAPAEKRVSKKK
                     G"
     gene            complement(1186..1605)
                     /locus_tag="VFF70_00210"
     CDS             complement(1186..1605)
                     /locus_tag="VFF70_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY43144.1"
                     /translation="MSKEGVVIILEDRSGCIAMQLRDDRLDVVAAGQWGLFGGWIESN
                     ESPRDAVVREVEEELNCALKVSKLSLLESRSINWGVPPDVFMMSTHVFHYPVTTECDH
                     AVLNEGQAFRFMQPIDLKDKWVVPHHWAILNDYWRTK"
     gene            complement(1721..3283)
                     /gene="guaA"
                     /locus_tag="VFF70_00215"
     CDS             complement(1721..3283)
                     /gene="guaA"
                     /locus_tag="VFF70_00215"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZY43145.1"
                     /translation="MREAIAIIDYGSQYTQLIARRVREAQVYCEIFAWDTAPDKVLAL
                     QPHAYILSGGPNSVYDPGAPTLPQYILDSHAPILGICYGMQMMAHTLGGKVAPSQQRE
                     YGPAEVQIIDEKLSLFSNLQHPTSNLQVWMSHGDRIDQVPAGFHVTARSTNSPIAAMA
                     DEQRQLYAIQFHPEVNHTPQGRAILRNFLFEVAHCTPAWTSTSFIEESVNSIRSRVGG
                     NQVICGLSGGVDSAVAAALIHKAIGDQLTCIFIDTGLLRQAEPEQIVDTFQKHMGLHL
                     IAVNAVEEFLSALDGVIDPEEKRKIIGEKFIRVFEREANAIKIDGRSPKFLAQGTLYP
                     DVIESRGPERQVADKIKTHHNVGGLPKDMQFELIEPLRYLFKDEARSIGSVLGLPDAI
                     VWRQPFPGPGLAVRIIGDITWDRLETLRAADAIVRAELDQAGLSREIGQSFAVLTPIR
                     SVGVMGDGRTYGNVVAVRAVTTDDFMTADWARLPYDVLGRISSRIVNEVKGVNRVVYD
                     ISSKPPATIEWE"
     gene            complement(3335..3907)
                     /gene="xpt"
                     /locus_tag="VFF70_00220"
     CDS             complement(3335..3907)
                     /gene="xpt"
                     /locus_tag="VFF70_00220"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002828841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HZY43146.1"
                     /translation="MNALQFRIKLEGKNLGRGILKVDAFINHQVDPQLMHSCAIEFAS
                     RFLSARPTKVLTAEISGIAPALLTGLELQVPIVYARKTKPITMPDTVILTTAPSHTKG
                     VMVDLMVSPEYLGKGERVLIIDDFLASGQTIMGLVRLAQAAGAEVIGIGALIEKTFEG
                     GREMLEQAGLRVESLVRITAMTDDQIVFAD"
     gene            4032..5216
                     /locus_tag="VFF70_00225"
     CDS             4032..5216
                     /locus_tag="VFF70_00225"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HZY43147.1"
                     /translation="MTLELNKVSHQVVQLGEQAAQRKRDLDLVVPQVQKILRAHANDA
                     DLRDLARRATETQKWRGAIPTAEPLDAAIDPPAHPDRLTIIAGDGSQIYPDAHGAALY
                     YLINVGTIIFKHGSDLAPIVATDPKVFGDAEALYEDDRLVSTNLVNARRNLSELAHVV
                     DLTLKENEASLAIALLDGTLALWAHAEGISQDQQDQLERDYIHRLDQLKDVHIPIGAF
                     VSRPRSTMVAELAALTDFDNSQDALTSIANNRVHRFKGLRDTAFFSSMLRTRQRSAVF
                     EVAPGWNTTYRDRGNSIHFFYVNVGDAHRSEIARVEVPVWVAEDRSALGLLHAAIVEQ
                     CTVSRGYPYVLARAHEIAVVTNGERAEFENMIATQLMRSGIEARPSEKAFQKSLLVGK
                     RR"
BASE COUNT         1234 a         1458 c         1419 g         1181 t
ORIGIN      
        1 attttttacc ggccgcggtg aagcccgcgc ccacgctcag cggctggttt tcttcgatcg
       61 cgttgatcat cttatacaac gcggcgcgcc cgaaggtgcc gatcaggaat gttacaaggc
      121 cgatcaccac gccaattccg atcagcgcat tgacatcgat cgattgaaac atctggcgca
      181 gcatttcgcg tccgccgagc ggcaggttgg cgaattggct ggtatcgccg ccacggaagt
      241 taatgctggc cgcgccgccg ccgagagcag ccagcaaccc aaagacccac aacgttctgt
      301 tcttccagaa gatcgaccac gatcgtttga acagcgacca ttgattcatg tgtccctaac
      361 ctttcttttt actcacacgc ttttcagccg gtgcggcttc aaccacagcc ggattctgcc
      421 attcacgata gaccagcgtc cagatcgtgg agatcaacac tgtcgtcaat gcaccgacga
      481 tcacgttcag cgtaatcaga attgtgccaa gcacggattg atcaaagtcg ggcacgttga
      541 tcaattggcc gaagatgact gcgacgatca tttccagtcc tatgatagcc acggccagga
      601 tcagtcccca ggtaatgaag tcaacgacct ttgttctcaa gagcagcaca ctctttttga
      661 gcgcagcgac gatcgcttga ccatcgatca cgatcgcgcg ctcggcgcca acactaatgg
      721 cgtggaccag cacagcgatc acgaatacaa caccgacgat actgagcagc ccgcccagat
      781 tatttagcaa gtccgaaatt tgtaaactgt tcggctgacc caagccgctg gcgatcacat
      841 cggtgatcga tccggtggct aggactaaga ggatccacac ggggatagcc aagatgaggc
      901 tggtgatcaa cagcggcgcg agattgcgca agcccgatcc aatcccggcg ccgatcgaag
      961 cgcgttcttc gctagccgcg ctattcacca ggctgatcaa cgcgccactg gtaaaagcag
     1021 tcacgatgaa agcgagcacc gtttgaagga tgcctactcc gatcgatggt tcgcgcagcg
     1081 catagctggc aatagcttga actaaaattc cgatcaaggt cacaatccat agtgccggag
     1141 tatgcagcca gatcgatccg gcgcgcttga gtaatgcagt gcgattcatt ttgttctcca
     1201 gtaatcgttc aagatagccc agtgatgggg tacaacccat ttatctttga gatcgatcgg
     1261 ctgcatgaat cggaaggctt ggccttcatt caacacagcg tgatcacatt cagtggtgac
     1321 gggataatga aaaacatggg tgctcatcat gaagacatcg ggcggcacac cccagttgat
     1381 cgatcgcgac tccagcaatg ataacttcga tactttaagt gcgcaattca actcttcctc
     1441 cacttcacgc acgaccgcat cgcgtggcga ttcattcgat tcgatccaac cgccgaacaa
     1501 accccactgt cccgcggcaa caacatccag tcgatcgtcg cgcagttgca tggcgataca
     1561 gcctgatcga tcttcgagaa tgatgacaac gccttcttta ctcaacttat tctcgcgtgt
     1621 gtcattccga ggccgtcggc cgaggaatct ctgccacgat cagcaactct agccgaggcg
     1681 atagagattc cgagcgaagc gaggaatgac aattcaattg ctactcccac tcaatcgtcg
     1741 cgggcggctt cgacgatata tcatacacca cgcggttaac gcctttgact tcattgacga
     1801 tgcggctgct gatgcgcccc agcacatcat acggcaggcg cgcccaatcg gcggtcatga
     1861 aatcatcggt cgtcacggcg cgcaccgcga cgacattgcc atacgttcga ccgtcgccca
     1921 tgacacccac gctgcggatc ggggtcagca ccgcgaagga ttgtccgatc tcgcggctca
     1981 gtccggcttg atcgagttcg gcccgcacga tcgcatcggc ggcgcgcagc gtttccaatc
     2041 gatcccaggt gatgtcgccg ataatgcgca ccgccaaacc cggcccggga aaaggctgcc
     2101 gccacacgat agcatccggc agacccaaca cactcccgat cgatcgcgct tcgtccttga
     2161 agagataacg cagcggctcg atcagctcga actgcatatc tttcggcagg ccgccgacgt
     2221 tgtgatgcgt tttgattttg tccgcgactt gacgctccgg cccgcgcgat tcgatcacgt
     2281 cgggataaag tgtgccttgt gccaggaatt ttggtgagcg gccgtcgatc ttgatggcgt
     2341 tcgcttcgcg ctcaaacacg cggataaatt tctcgccgat gattttacgc ttctcttcgg
     2401 gatcgatcac cccatcgagc gcggataaaa attcttcaac ggcgttgacc gcaattaggt
     2461 gcagacccat atgtttttga aaagtatcga caatttgttc aggctcggct tgacgcagca
     2521 agccggtatc gatgaagatg caggtcagtt gatcgccgat cgctttgtgg atcaacgcgg
     2581 cggctacggc cgaatcgacg ccgccggaaa gcccgcagat cacttgattg ccccccacgc
     2641 gggatcgaat agagttcact gattcttcaa tgaacgaagt tgaggtccag gcaggcgtgc
     2701 agtgtgcaac ctcgaacaaa aagttgcgga ggatcgcgcg tccctgaggt gtgtgattca
     2761 cttcgggatg aaactgaatc gcgtacaact ggcgctgctc atcggccatc gccgcgatcg
     2821 gcgagttggt cgatcgggcg gtgacgtgaa agccggctgg cacttgatcg atccgatcgc
     2881 catgggacat ccacacttga agattggatg ttggatgctg gaggttggag aagagagaca
     2941 acttctcgtc gatgatctga acttcggctg gaccatattc gcgttgctgc gaaggcgcga
     3001 ctttgccacc gagtgtatgg gccatcatct gcatgccgta gcaaatgccc aagatcggcg
     3061 cgtgtgaatc gagaatgtat tgaggcaacg tcggcgcgcc gggatcgtac acgctgttcg
     3121 gcccgcccga taaaatgtaa gcgtgaggtt gaagcgcgag aactttatcg ggcgcggtat
     3181 cccacgcaaa aatttcgcag tacacctgcg cctcgcgcac acgccgcgcg atcaactgcg
     3241 tgtactgcga tccgtaatcg ataatggcaa tggcttcgcg cattaattct ccatgaaaat
     3301 gaatccagat tctttagcgg gaatctggtt tcacttaatc tgcgaacacg atttggtcgt
     3361 cggtcatcgc cgtaatgcgg accagcgatt cgacgcgcaa gccggcctgc tccaacattt
     3421 cgcggccgcc ctcgaaagtt ttttcgatca gcgcaccaat gccaataact tccgcgccgg
     3481 ccgcttgggc gagccggact aaacccataa tcgtttggcc gctggccagg aagtcgtcga
     3541 tgatcaacac gcgctcacct ttgcctaaat attcgggtga caccatgaga tcgaccatca
     3601 cgcctttcgt gtgagacggc gcggtggtga gaatgaccgt gtccggcatg gtgatcggtt
     3661 ttgttttgcg ggcatatacg atcgggactt gcagctctaa cccggtcaat aaggcgggcg
     3721 cgatgccgct gatttcggcg gtcaacactt tggtcggacg ggctgacaga aagcggctgg
     3781 caaactctat ggcacagctg tgcatcaact gcggatcgac ttgatgattg atgaaggcat
     3841 caaccttgag aatgccgcgt cctaagtttt tgccttcgag cttaattcga aattgcaatg
     3901 cgttcatgcg aggctccaga ggtaggatac cgaattttac atcagtcagg ttgaatctac
     3961 aacgaggctt gattttgatc ggcggcttgg ttataatcaa cccactttcg atcggcttcc
     4021 cctaaaagac catgaccctt gaactcaaca aagtcagtca tcaagtggtt caactcggcg
     4081 agcaggccgc gcagcgtaag cgcgatctcg atctcgtcgt accgcaggtg caaaagattc
     4141 tgcgcgcaca tgcgaacgat gccgacctgc gcgatctcgc tcgacgcgcg acggaaactc
     4201 agaaatggcg cggcgccatc cccaccgctg aaccactcga tgctgcgatt gatccgcccg
     4261 cacaccctga tcgattaacg atcatcgccg gcgatgggtc acagatctat cccgatgcgc
     4321 acggcgcggc gttgtattat ttgatcaacg tcggcacaat catcttcaag cacggttccg
     4381 acctggcccc gattgttgcg actgacccga aagttttcgg cgacgcagaa gcattatatg
     4441 aagatgaccg acttgtgtct actaacttgg taaatgcccg acgtaattta tccgaattgg
     4501 cgcatgttgt tgatttaaca ctgaaagaga atgaagctag cctcgcaatt gcattactcg
     4561 atggaacgtt ggcactttgg gcgcatgccg aaggaatctc acaggatcag caagatcaat
     4621 tagagcgtga ttacatccat cgacttgatc aattgaaaga tgtgcacatt ccgatcggtg
     4681 catttgtttc ccggccacgc agcacgatgg tggcagaatt agctgcgttg actgactttg
     4741 ataattcaca agatgctcta acgagcatcg ctaataatcg cgtccatcgt ttcaagggac
     4801 tacgtgatac cgcattcttc tcctcaatgc tgaggactcg tcagcgtagt gcggtctttg
     4861 aagttgcgcc aggctggaat acgacatatc gcgatcgagg taatagcatt cacttcttct
     4921 atgtgaatgt cggtgatgcg catcgttccg agatcgcgcg cgtggaagtg cccgtttggg
     4981 tcgccgaaga tcgatcggcg ctgggcttgc tccatgcggc gatagtcgag cagtgcaccg
     5041 tctcgcgcgg ttatccgtac gttttggcgc gcgcgcatga aatcgccgtg gtcaccaatg
     5101 gcgaacgcgc cgagtttgaa aacatgatcg ctacgcagtt gatgcgatca ggcatcgaag
     5161 cgcggccatc ggaaaaagcg tttcagaaat cgttgttggt aggtaagcgc agatgaaaat
     5221 gtgaagcgat aaacgcgctc agggtggccg tggggatgta cgaacttgta cagggtggag
     5281 actgaatatg at
//
LOCUS       DASRTJ010000014         1789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_708,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000014 DASRTJ010000000
VERSION     DASRTJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFF70_00230"
     CDS             complement(<1..584)
                     /locus_tag="VFF70_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43148.1"
                     /translation="MPYHILMIAPTSFFGDYGCHVRILEEARILQRRGQRVTIVTYYQ
                     GRDLPELKIIRTRPTPWHADYEVGSSRHKLAFDVLLSWTALRAALKLKPDIIHAHLHE
                     GALIGRVLGALTGAPLVFDFQGSLTAEMLDHHFIRPGGKRAWAVRRLENAIDRMPRAI
                     LTSSQHSAKVLLNEFNLQPDRVIPLPDCVDTEFFR"
     gene            complement(712..972)
                     /locus_tag="VFF70_00235"
     CDS             complement(712..972)
                     /locus_tag="VFF70_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZY43149.1"
                     /translation="MPYVYIVECTDGSLYTGWSIDVEARVKAHNAGRGGRYTRTHRPV
                     KLVYLESHRTRGAAMKREVAIKTWPRKRKIQLISDQGSKKNK"
     gene            complement(998..>1789)
                     /locus_tag="VFF70_00240"
     CDS             complement(998..>1789)
                     /locus_tag="VFF70_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZY43150.1"
                     /translation="LIIITSPASELREIFLEIGSQLLEGAVVIDLASVKSEVLKIAEE
                     ALPKTVHHLGCHLILHPDASDRFPAEPDADLFHGSILVMTPAIHTDEVAIKSGSSLAK
                     TIGARPYFMDVFEHDGLLAAVEGMPGLVSAAMLLAATRSRAWDELSQIAGAIFAQATQ
                     PAGHPAIDGGTALTMNKAEVLRWLDTFLETLREVRQTVSDGNAKQINTLLAAATQQRL
                     EWLAARPMTPWNDEEAMPGPPHELRRMDLLAPKWGQEFKEPQRKS"
BASE COUNT          393 a          517 c          495 g          384 t
ORIGIN      
        1 cgaaaaaatt cggtgtccac acaatcgggc agcggaatca ctcgatcggg ctgtagattg
       61 aattcattca gcagcacctt cgcgctgtgc tgcgaggaag tcaagatcgc gcgcggcatc
      121 cgatcgatcg cgttctccaa tcggcgcaca gcccatgcgc gtttgccgcc cggtcggatg
      181 aagtgatgat ccaacatttc ggcggtcagg ctgccttgaa aatcaaacac gagcggcgcg
      241 ccggtcaacg cgcccaacac ccggccgatc aatgcgcctt cgtgcaaatg cgcgtgaata
      301 atatcgggtt tcaatttcaa cgcggcgcgc agcgccgtcc acgacaacaa cacatcgaac
      361 gcgagtttgt ggcgcgacga tccgacttca tagtcagcgt gccacggggt gggccgcgtg
      421 cgaatgattt tcagttcggg caggtcgcgg ccttggtaat aggtcacaat cgtcacgcgc
      481 tgcccgcgcc gctgcaaaat gcgcgcctct tcgagaatgc gcacatggca accatagtcc
      541 ccaaagaaag acgtcggggc gatcatgagg atgtggtagg gcataaggat aaaggagaaa
      601 cccctctccg cttcgctacg ggggcgcttc gcgggatgaa ggatgaaaga aaaacgaaga
      661 gcggtcgatg attagtgaag agtgactagt aaaatcgctg caaccttatc actacttatt
      721 tttctttgat ccttgatcac tgattaactg tatttttctt ttcctcggcc acgttttgat
      781 cgcgacttcg cgtttcatcg cggctccacg cgtgcgatgt gattccaaat aaaccaactt
      841 caccggtcga tgggtgcgtg tataccgtcc gcctcgaccg gcgttgtgcg ctttcacgcg
      901 cgcttcaaca tcgatcgacc agccggtgta taacgatcca tctgtgcact cgacgatgta
      961 aacgtagggc atgtcatgaa ggagggctga aagcggatca agatttacgc tgcggctctt
     1021 taaactcttg accccacttc ggcgcgagca gatccatgcg gcgcagttcg tgcggcggtc
     1081 cgggcatggc ttcttcatcg ttccatggcg tcatcggccg cgccgccaac cattccaaac
     1141 gctgctgcgt cgcagcggcc aacagcgtgt tgatctgctt ggcattgcca tcgctcacgg
     1201 tctgtcgaac ttcgcgcagt gtttccaaga acgtatccaa ccagcgcagc acttccgctt
     1261 tgttcatcgt caaggccgtg ccaccgtcga tcgccggatg gccggcaggc tgcgtcgctt
     1321 gcgcgaagat cgcgccggcg atctggctga gttcatccca cgcgcgcgat cgagttgcgg
     1381 ctagcagcat tgcggcggac accaagccgg gcatgccttc aaccgcggcc aacagtccat
     1441 cgtgctcgaa cacatccata aagtatggcc gcgcgccgat cgtcttggcc aagctgctgc
     1501 cggacttgat cgcaacttca tcggtatgaa tggccggtgt catcaccagg atcgatccgt
     1561 ggaacagatc ggcatccggt tcagcgggga atcgatcaga agcatcggga tgcagaatca
     1621 agtggcagcc caagtgatga acggttttag gcaatgcttc ttccgcaatc ttcagcactt
     1681 ctgatttcac cgatgccaga tcgatcacaa ctgcgccttc gagcagctgc gaaccaatct
     1741 ccaagaagat ctcgcgcaat tcagacgcgg gtgacgtgat gatgatcaa
//
LOCUS       DASRTJ010000015        10226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_265224,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000015 DASRTJ010000000
VERSION     DASRTJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265224"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFF70_00245"
     CDS             <1..414
                     /locus_tag="VFF70_00245"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZY43151.1"
                     /translation="PIMHYRLSKHDGDLIKRGLIETVRLHVAAGAQEVGGPITGLPTY
                     RSANGELEAYLDRIQPMMLRPNGSMLFSAHQMSTCHMGADRSSSVIDEHCEAHEVKGL
                     FVTDGSSLPNAPGVNPMITIMAVAYQAAQYIKTRC"
     gene            586..1200
                     /locus_tag="VFF70_00250"
     CDS             586..1200
                     /locus_tag="VFF70_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZY43152.1"
                     /translation="MNSIDQIIQRVKKTQHGFGDIRTAATEIVAEHSRTESLRIAKTL
                     FTSEVHQARAAAVFMMGDLAAQSKSTLKFLRSKVSQDSDWRVQEILAKAFDRYCADVG
                     YETALPVIKDWLQDKQPNVRRAVTEGLRIWTTRPYFKDQPEVAIDLLSRLKDDDSDYV
                     RKSVGNALRDISRRHKDLIRAEVLTWDVSNRYVKQTQQLASKFL"
     gene            1382..2884
                     /locus_tag="VFF70_00255"
     CDS             1382..2884
                     /locus_tag="VFF70_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZY43153.1"
                     /translation="MINRNIDGYEITQEVGRGNAAAVYAARQQPVGRYVAIKIFDHLT
                     SDSAARLEQLFTQLEAIDHVNILPMYDSGQIDDRVYWVMRYMPTGPLKTRSHWTIDQI
                     DQLIGQVASALDYAHQHDLVHGSLKPSNVLLDHAGNAFVCDFGVAHIVGQSPSDYPLP
                     EQRRDFKPDVRSDVYALGALAYELLTGRPPIDLRGREQDRLNWRVTLPPLPSSINSKL
                     SPAIDAAVMKALSIDPAQRYPSAPEFAEAFTQVRGTVEAVQDETQPLDRVSDSTARRV
                     RTAHAKIDWRWIAGGFIGLLILIGLSSVLSSPPQATVIQSTSTPVSIVTAVSTSIPLA
                     VPRPSSVPTSEPSVTPGSIVSATPIISVPSTSAPTPKPPLAPTVPVRASVTPTPTVPI
                     SIESLTLLIPRKADRATLRLHFHTHIRPDNAGPIGMLSMSIPAIEPLIKERGLAAVGS
                     GDQTLGVSIVLSCNLLADPITTRQILLIVKDAQGKTIYAQSLDYTKTWCQ"
     gene            2932..3705
                     /locus_tag="VFF70_00260"
     CDS             2932..3705
                     /locus_tag="VFF70_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY43154.1"
                     /translation="MDYTTILTSISDRVGLVQINRPQQMNALNATVMEELAIALEAFD
                     RNDQAGCLVVTGNQRAFAAGADIKEMSDASAIEMLTRDTIARWDRIRQIKKPIVAAVS
                     GYCLGGGCELAMGCDLIVASESAQFGQPEINLGVMPGAGGTQRLTRAIGKSKAMEMIL
                     TGRTMSAREAEQCGLVVRVVPIEVYLDEAIKLANEIAARPPIAVRFAKEAINKAFETS
                     LADGLEYERRLFYFLFATEDQKEGMQAFIEKRKADWKGK"
     gene            3988..4599
                     /locus_tag="VFF70_00265"
     CDS             3988..4599
                     /locus_tag="VFF70_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZY43155.1"
                     /translation="MTKKSQSTDQPKSKHPLLFKIAIILGGLLVLGTAGGVTAMTLEN
                     HDSFCASCHAEPEYTYFQRESSTTPVDLATFHTTKQANCIDCHSGNGIVPGRINSLML
                     GARDLVSWIGGHAPQPAPLNTPIGDENCLKCHSNIAAIQNMNNHFHVFLAQWQAKDPN
                     AAQCVSCHQGHNTTTADSTLSFLDRQATTAVCQRCHTFSGEGN"
     gene            4787..6496
                     /locus_tag="VFF70_00270"
     CDS             4787..6496
                     /locus_tag="VFF70_00270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZY43156.1"
                     /translation="MFKNLPTEPQGFIDWTWSNYEPYYHDLETRSLDGSTVAQWLADW
                     TSISQRFSETYSRLHVATTLNTADTEAEQRYHAFIGNVVPAIEAADQKLKLKLLASQL
                     QPIGFEIPLRNMRAEADLFREENLPLLTENRKLGSEYNKIIGAQSIQWNGVEITLQQV
                     RPIIQNSDRVTREKAWRAAADRQLASRTAINELWVKLMKVRGQIPRANGLPNFLEYKW
                     RELLRFDYSPADSETFQRAIESVAMPAATRIYNKMRDRLGVKTLRPWDLDLDLYPISF
                     PPLKPYASIEELLDKPSAIFHRVDPQLGEYYDIMRREKLIDPSNYKGKAPGAYCTEYT
                     VQKRPFVFLNSVGVRDDVRTLLHECGHAFHVFETNHLPYIQQLQVTMEFAEVASMSME
                     LLAGPYLSKQNGGFYSEAEATHDRIDHLERNILFWPYMAVVDAFQHWVYTRIEEATDA
                     GNCDRKWAELWRRFIPGVDWSGLEDSMMTGWHRKQHIHRAPLYYVEYGLAQLGAVQVW
                     RNALKDQASAVKNYRHALSLGATVALPELYKAAGAKFAFDAHTLGEAVELIESQILQL
                     EKN"
     gene            6646..7611
                     /locus_tag="VFF70_00275"
     CDS             6646..7611
                     /locus_tag="VFF70_00275"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY43157.1"
                     /translation="MTTCLVLGATGFIGGQIVRSAMARGWQVRALRRDPKSVGAIGDL
                     KPDWFVGNLDDRSSLLEAMRDCDVVFHAAAAYPHAAHGIDRRVQAAVAQMQNVLSAAT
                     EAKLDRLIYTSSFTTIGSPGDPNRFADERDQYQPGSAHDAYYESKWAMEVAALNAAHA
                     GLPVIALCPAAVFGPGDVHLSVSEIIVMTAKGKMPFYLDATFGAIDVRDVAEAHLNAI
                     ERGTIGERYILSAHNITLQEGLTLAARAANQKPPSIKIGRRLLTAIIAVGQWLPGGSI
                     GHLRTMRFWQPLNNAKAINELGLTTRPIDQTLNDALTWFKANHYL"
     gene            7670..8041
                     /locus_tag="VFF70_00280"
     CDS             7670..8041
                     /locus_tag="VFF70_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43158.1"
                     /translation="MNYTEPEVITIIEGPPPDIQLSQEMWSFSLWEGTAPRSMGVCQM
                     RTFNGSSMMDRCTGAWREGRDVRLDFPQRDGLRKQVDVLAARTDQTPDGDVLFLWIVA
                     PLGEEIESEAEDADDDPGSLA"
     gene            8118..8534
                     /locus_tag="VFF70_00285"
     CDS             8118..8534
                     /locus_tag="VFF70_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZY43159.1"
                     /translation="MKPDSTAIEIPATHIDLFKRATKAFAMLALTLSDGSPHVTPIWF
                     DWDGTAIIINTARNRVKDKILRKHPKVAVLISAPDDPYRYIHLRGRVVEETEEGGYEE
                     ICDLNEKYHGNRNFLKRPGQVRVTYKILPEKIFADT"
     gene            complement(8625..9146)
                     /locus_tag="VFF70_00290"
     CDS             complement(8625..9146)
                     /locus_tag="VFF70_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZY43160.1"
                     /translation="MLNVLLLNASFEPLAVVGLKRAIGLIIDGKVDLVEAVPGRELRS
                     PSTRQPMPSVLRLRYFVNVPRRGVGWSRRGVLSRDHHSCQYCGRHMRPGETTIDHIIP
                     QWQCKRLNIRASTWGNTVACCPKCQQRKGGRSMHEAGIRFFNPNYEPKMPRTSYLVMT
                     SSIRPEWRQYIRL"
     gene            9582..>10226
                     /locus_tag="VFF70_00295"
     CDS             9582..>10226
                     /locus_tag="VFF70_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HZY43161.1"
                     /translation="MAHSLQIFIDEYVQQLEPLLKLRNAAYWDFTAQGDEQIQQEYAR
                     LDTEIRQIHSDKARFEELKKLSATPSGDALLDRQARLMRDSFQSQQMSPDMIKQIVAL
                     ETDITAEFNGFRADLNGQSMTDNQLKKILKESNDEVERRAAWEASKQIGAQVADRVRQ
                     TVQLRNRAAREMGFANYYAMSLHLQELNEDTLFALFDRLEKLTRPLFTAYKTDLD"
BASE COUNT         2396 a         2723 c         2868 g         2239 t
ORIGIN      
        1 ccgattatgc attatcgact ttcaaaacat gacggtgatc tgattaagcg cggcttgatt
       61 gaaacggtgc gattgcatgt ggcggccggc gcgcaggaag ttggcgggcc gattacgggt
      121 ttgccgacct atcgatcagc caatggagag ttggaggcgt atctcgatcg aatacagcca
      181 atgatgctgc ggccgaacgg ctccatgttg ttcagcgcgc atcaaatgag cacttgtcac
      241 atgggtgctg atcgatcgtc gagtgtgatt gatgaacatt gtgaggcgca tgaagtgaag
      301 ggcttgtttg taaccgatgg cagttcgctg ccgaatgcgc ccggcgtcaa tcccatgatc
      361 acgatcatgg ccgtggccta tcaggcggcg caatatatta aaacgcgctg ctgaatgtgg
      421 agtatggaat gcagaacgcg aaaagcatgt tgtcattctg agccgcggcc ctgcacaaaa
      481 ctggcagggc cttcgggcgg cgaagcaatc tctatcgcat gagcgctctg cgccgatcag
      541 catagagatt cctcgctgtg cccggaatga caggatcaat ttgtgatgaa ctcaatcgat
      601 cagatcattc agcgtgtcaa gaagacgcag cacggctttg gcgatattcg cacagcggcg
      661 acggagattg tggccgaaca ttctcgtacc gaaagtttac gcatcgcgaa aactctattc
      721 acttccgaag tgcatcaagc ccgcgcggca gcggtgttca tgatgggcga ccttgccgcg
      781 caatcgaagt caacgctgaa attcttgcgc tcgaaagtaa gtcaggatag tgattggcgt
      841 gttcaagaaa ttttggcgaa agccttcgat cgatattgcg cggatgttgg atatgagacg
      901 gctttgcccg tgattaaaga ttggctgcag gacaagcagc caaatgtgcg gcgcgcagtc
      961 accgagggtc tgcgtatctg gacaacgcgg ccctatttca aagatcagcc tgaagtggct
     1021 attgacttat taagccgatt gaaagatgat gacagtgatt acgtccgcaa gtcggtgggc
     1081 aatgcgctgc gcgacatcag ccgcaggcac aaggatctga tccgtgccga agtgctgacg
     1141 tgggatgtgt cgaatcgata cgtgaaacag acgcaacaat tggcgagcaa gtttttgtag
     1201 agcgtggaat acggagcgga acgtcaatgt gagcaacacg tgcaccgcaa tgtcctgcgc
     1261 tggtgcaacc gcaggctgtg taacacggag cagtgcaggt gggcgaagaa gtctcattga
     1321 tagtgagtcg agattgcttc gtcggcctga cggcttcctc gcactgacgc ccggttaaaa
     1381 aatgatcaac cgaaacattg acggctacga aattacgcaa gaggtcgggc gcggcaacgc
     1441 ggctgcggtg tatgctgctc ggcagcaacc cgttggacgc tatgtggcga tcaaaatttt
     1501 cgatcacctg acgtctgatt cggcggcgcg cttggaacag ctattcactc agctcgaagc
     1561 gatcgatcat gtgaacattc tgccgatgta cgacagcggg cagattgatg atcgggtgta
     1621 ctgggtgatg cgctatatgc cgaccggccc attgaaaacc cgatcgcact ggacgatcga
     1681 tcagatcgat cagctgatcg ggcaagtcgc gtccgcgctg gattacgcgc atcagcacga
     1741 tctggtgcat ggcagtctga agccgtcgaa cgtgctgctc gatcatgcgg gcaatgcgtt
     1801 cgtgtgcgat tttggcgtgg cgcacatcgt cgggcaatcg ccgagtgatt atccgctgcc
     1861 cgaacaacgc cgtgatttca agcccgacgt gcgatccgat gtctacgcat tgggcgcgct
     1921 ggcgtatgag ctgttgaccg gccgtccgcc gatcgatcta cgcgggcgcg aacaggatcg
     1981 gttaaattgg cgcgtgacgc tgccgccgct gcccagttcg attaattcca aactttcacc
     2041 tgcgatcgat gccgcagtca tgaaagcact ctcgatcgat ccggcgcagc gttatccatc
     2101 cgcgccggaa ttcgcggaag cattcaccca ggtgcggggt acagttgaag cggtgcaaga
     2161 tgaaacgcag ccgcttgatc gtgtttcgga ctcaacggcg cgccgcgtcc ggacagccca
     2221 cgcaaaaatc gattggcgtt ggatcgcggg cggtttcatc gggctgctga tcctaatcgg
     2281 gttaagcagc gtcctttcat caccacctca agccacagtt attcaatcga ccagcacacc
     2341 tgtgtcgatc gtcactgcag tatcgacatc aattcccctc gcagtgcctc ggccatcgag
     2401 cgtgccgacc agcgaaccaa gtgttacacc tggctcgatc gtttccgcca cgccgatcat
     2461 ttctgtgccg tcgaccagtg cgcccacgcc taagccgcca cttgcgccca ccgtccccgt
     2521 gcgggcgtcg gtcacaccga cgccgactgt gccgatcagc atcgagtcgt tgacgctgct
     2581 gattccgcgc aaggccgatc gcgcgacgtt gaggctgcat ttccacacgc acattcggcc
     2641 ggacaatgcc gggccgatcg gtatgttgtc gatgtccatt ccagcgattg agccattaat
     2701 caaagagcga ggtttagcgg cagtcggatc gggcgatcag acgctgggcg tatcgattgt
     2761 gctgagttgc aatctgctgg cagatccgat cacaacgcgg cagatattac tgattgtgaa
     2821 ggatgcacag ggcaagacga tttatgcgca gtcattggat tacacgaaga cctggtgcca
     2881 gtaaccccag cctagccccc tcatcgcagc gaggaaggcg aggatcgttt aatggattac
     2941 acaactatct taacttcaat ttcagatcgc gttgggctgg tgcagatcaa tcggccgcag
     3001 cagatgaacg cgctgaatgc caccgtgatg gaagagctgg cgatcgcgct ggaagcattc
     3061 gatcgcaatg atcaagcggg ctgcctggtg gttaccggca atcaacgcgc gttcgcggcg
     3121 ggcgcggata tcaaagagat gtcggacgcc agcgcgattg agatgttgac acgcgacact
     3181 attgcgcgct gggatcgtat caggcagatc aagaagccga tcgtggccgc cgtgtccggt
     3241 tattgtttgg gcggcggctg cgaattggcg atgggctgtg atctgatcgt cgcatcggaa
     3301 tcagcgcagt tcggtcagcc ggagatcaac ctcggcgtga tgccgggcgc gggcggcacg
     3361 cagcgactga cccgcgccat cggcaaaagc aaagcgatgg aaatgatctt gaccggtcgc
     3421 acgatgtcgg cgcgcgaagc ggaacaatgc ggacttgtcg tgcgcgttgt gccgatcgaa
     3481 gtttatttgg acgaagcaat caagttggcg aatgagatcg cggcgcggcc gccgatcgcg
     3541 gttcgatttg cgaaggaagc gatcaacaaa gcgtttgaaa cttcactcgc cgatggatta
     3601 gaatacgagc ggcgtttatt ctatttctta ttcgcgacgg aagatcaaaa agaaggcatg
     3661 caggcgttta tcgagaaacg caaggcggac tggaaaggaa agtaggggca aggtgacctc
     3721 gttcgtacga acagacgaaa catgatcatc tctcacgcag aaatcatcac aggtctgcag
     3781 tcgatcggcc tgccgagcgg tatcaacgtt ttagatcatt cgttattagc gtcgattatg
     3841 cctcgatcga ttacaagtcg atcaactagc tcgcaatgac agacgggcct ttaaactctt
     3901 catccaacct taacatcccc ttcatcactt ccacaacaaa attatccata atgaacgcca
     3961 acatctatca aggcgcttat cttcagcatg actaaaaaat cccaatcgac cgatcagccc
     4021 aaatccaaac atccactgtt attcaagatc gccatcatcc tcggcggatt gctagtattg
     4081 ggaacagcgg gtggcgtcac tgcgatgacg ctggagaatc acgattcgtt ttgcgcgtcg
     4141 tgccacgccg agccggaata tacttacttt cagcgcgaat cctcaaccac gcccgtcgat
     4201 ctggctacct tccatacgac gaagcaagcg aactgcatcg attgccactc cggcaatggc
     4261 attgtgccag gccgtatcaa ttccttgatg ttgggcgcgc gcgatctggt ttcatggatc
     4321 ggcgggcatg cccctcaacc cgcgccgctc aacaccccga tcggcgatga gaattgtttg
     4381 aagtgccaca gcaatattgc cgccatacaa aacatgaaca atcactttca cgtgttcttg
     4441 gcccaatggc aagccaaaga tccaaatgcc gctcagtgcg tcagctgcca tcaaggccac
     4501 aacaccacca cggctgatag cacgctttca ttcttggatc gacaggcgac gaccgccgtg
     4561 tgccagcgct gccatacttt ctccggcgaa ggcaactaaa tatttcaccg ccgagtacac
     4621 taagagcgca gagaaaataa acttctctgc gctcttagtg tctgcctatg cccgacgcag
     4681 agcgtcggtg ggccgctagg cgcagcgatt tatttccttc gattgacaac ctctgcactc
     4741 gatgataggc ttcccatgtt gaaactttac ctgaagggac acattcatgt ttaaaaattt
     4801 gccgactgag cctcagggtt ttatcgattg gacttggtcg aattacgagc cgtattatca
     4861 cgatctggaa actcgatcgt tagacggcag cactgtggcg cagtggctag ccgattggac
     4921 ctccatcagc cagcgatttt ctgaaaccta ctcgcgctta catgtcgcca cgacattgaa
     4981 caccgccgat accgaagccg agcagcgcta tcacgctttc atcggcaatg ttgtccccgc
     5041 gatcgaagcg gcggaccaga aactgaaatt aaagttactg gcgagccagc tgcagccgat
     5101 cgggtttgag attcccctgc gcaatatgcg agctgaagcg gatttgttcc gtgaggaaaa
     5161 tctgccgctg ctgacggaaa atcgaaaact cggatcggag tacaacaaga tcatcggcgc
     5221 gcagagcatt caatggaacg gcgtagagat taccttgcag caggtcaggc ctatcatcca
     5281 gaattccgat cgagttacgc gcgaaaaagc ctggcgggcg gcggctgatc gccagttggc
     5341 atcgcgcacg gcgatcaacg agttgtgggt gaagttgatg aaggtgcgcg gacaaattcc
     5401 gcgcgcgaat ggcctgccga attttctgga atacaaatgg cgcgaacttt tgcgcttcga
     5461 ttattcaccg gccgatagcg aaactttcca gcgcgcgatc gagagtgtgg ccatgccggc
     5521 ggctacgcgt atctataaca aaatgcgcga tcgattgggc gtgaaaactt tgcgaccgtg
     5581 ggatcttgat cttgatctgt acccgatcag ctttccgccc ctcaagccgt acgcttcgat
     5641 cgaagaattg ctggacaaac cctcggcgat tttccatcgc gtcgatccgc agttgggcga
     5701 atattacgac atcatgcgac gcgaaaaatt gatcgatccg tcgaactata aaggcaaagc
     5761 gccgggtgca tactgtacgg aatacaccgt gcagaagcgg ccgttcgttt tcttgaattc
     5821 tgtcggtgtg cgtgatgacg tgcggacttt gctgcacgag tgcggtcatg cattccatgt
     5881 ctttgaaacg aaccatctgc cgtacatcca acagctgcaa gtcaccatgg aatttgccga
     5941 agttgcctca atgagtatgg agctgctggc cgggccgtat ctctcgaagc agaacggcgg
     6001 tttctattcg gaggccgagg ccacgcacga ccgcatcgat catttggagc gcaatatatt
     6061 attctggccg tatatggccg tggtcgatgc attccagcat tgggtctaca ctcgcattga
     6121 agaggcgacg gatgcgggca actgcgatcg aaagtgggcg gagttgtggc ggcgcttcat
     6181 tcccggtgtc gattggagcg gcctcgaaga ttcgatgatg accggctggc atcgcaagca
     6241 gcacattcat cgcgcgccgt tgtattacgt ggaatatgga ctggcgcagt taggcgcagt
     6301 gcaagtctgg cgcaacgcct tgaaagatca agcgagtgcg gtgaagaatt atcgacatgc
     6361 gttgtcactc ggcgcaacag ttgctttgcc tgaattgtat aaagccgctg gcgcgaagtt
     6421 cgctttcgat gcacacactt tgggagaagc cgttgagttg atcgaatcac agattttgca
     6481 gttagagaag aattgatcca cgaagaagat aaagtgtcat tgcaagccgc tcagcggcga
     6541 agcactctcc cgatcgattt gctcaaccga cgagattgct tcgtggcaca tccctagagg
     6601 gctgtgccat ttagggactt cgcttgcaat gacgcccatt tccttatgac cacctgcctc
     6661 gttctgggcg cgaccggctt catcggcgga caaattgttc gatcggcgat ggcacgcggc
     6721 tggcaagttc gtgcactgcg acgcgatccc aaatccgtcg gcgcgatcgg cgatctgaag
     6781 cccgactggt tcgtcggcaa cctcgatgat cgatcttccc tgctggaagc catgcgcgac
     6841 tgcgatgtgg tgtttcatgc ggccgcggct tatccgcatg cggcacacgg catcgatcgg
     6901 cgcgttcaag cagctgtggc tcaaatgcaa aatgtattga gtgcagctac agaagccaag
     6961 ctcgatcgct tgatctatac gagttccttc actacgatcg gttcaccggg cgaccccaat
     7021 cgattcgcag atgaacgcga tcagtatcag cccggctcag cgcacgatgc ttattacgaa
     7081 tcgaaatggg cgatggaagt cgccgcgttg aatgcggcgc atgcaggttt gccggtcatc
     7141 gccttgtgcc cggcggcggt gtttggccca ggcgatgtgc atctctccgt aagtgagatc
     7201 atcgtgatga cggcgaaggg gaagatgccg ttttatcttg atgccacatt cggcgcgatc
     7261 gatgtgcgcg atgtggctga agcgcacctc aacgcgattg agcgcggaac gatcggcgag
     7321 cgctacatcc tctccgcgca caacatcact ttgcaagaag gcttgacgtt ggcggcgcgg
     7381 gccgccaacc agaaaccgcc atcgatcaag atcggccggc gtttgctcac agcgatcatt
     7441 gccgtgggtc aatggctgcc cggtggatcg atcgggcatt tgcgcacaat gcgtttctgg
     7501 cagccgctga ataatgccaa agcgatcaac gaattgggtc tgaccacgcg cccgatcgat
     7561 caaactttga atgacgcctt gacgtggttc aaggcgaatc attatttgta agtttgatta
     7621 aaatcgatat ggtataatct ccccgcaatc tcaggtagga gcagttcaca tgaattacac
     7681 agaaccggaa gtcattacga ttatcgaagg cccgccgccg gatattcaac tctcacaaga
     7741 gatgtggtcg ttctcgctgt gggaaggcac cgcgccgcga tcgatgggcg tctgccagat
     7801 gcgcaccttc aacggatcgt cgatgatgga tcgctgcaca ggcgcgtggc gtgaaggccg
     7861 agacgtgcgc ttggattttc cgcagcgcga tggtctgcgc aagcaggtgg atgtgctggc
     7921 cgcccgcacc gatcagacgc ccgatggcga tgtgttattc ctgtggatcg ttgcgccgct
     7981 cggggaagag atcgaatccg aggcggaaga cgcagatgac gatcccggtt ccttggctta
     8041 actgatctcg tgctgcaaac tggatagcga cggctatcca gtttttgttt accccattca
     8101 tcgtaaagga ttagaatatg aaacctgact caacagcgat tgaaattccc gcgacccaca
     8161 tcgatttgtt caagcgcgca acgaaagcct ttgccatgtt ggcgctgacc ctgtcagacg
     8221 gatcgccgca cgtcacaccc atttggttcg attgggacgg gactgccatt atcatcaaca
     8281 ccgcgcgcaa tcgagtcaaa gataaaatat tgcgcaagca tcccaaagtt gccgtgctga
     8341 tctctgcgcc cgatgatccg tatcgctata ttcatttacg cgggcgagtc gtagaggaaa
     8401 cagaagaggg cggttacgaa gagatttgcg atctcaatga gaagtatcac ggcaaccgca
     8461 attttctgaa acggcccggg caagtacgcg tcacttacaa gatattgccc gaaaagattt
     8521 ttgctgatac gtaagcggca aaaaaagacc tgcgcggttt ctaaaaccgc gcaggtctaa
     8581 actttctaaa gtccgcgctg cagataaact gtggcgcgtt ttatttacaa gcgaatgtac
     8641 tgccgccact ccggccggat gctggaggtc atcaccaagt aactggtgcg cggcatcttc
     8701 ggttcgtaat ttggattgaa gaaccggatg ccggcttcgt gcatcgatcg gccgcccttg
     8761 cgctgctggc acttcgggca gcaggccacg gtgttgcccc acgtgctcgc gcgaatattc
     8821 aagcgcttgc actgccactg tggaatgatg tgatcgatcg tggtttcgcc ggggcgcata
     8881 tgcctgccgc agtattggca gctgtgatga tcgcgcgaca gcacgccgcg ccgtgaccaa
     8941 cctacgccgc gtcgcggcac attcacaaag taacgcagcc gcagcacgct gggcatcggc
     9001 tggcgagtgc tgggcgagcg cagttctcgg cccggcaccg cttccaccaa atccactttg
     9061 ccgtcgatga tcaagccgat ggctcgcttc agcccgacca cggccagcgg ttcaaatgag
     9121 gcgttcaata acaagacgtt caacatagtt gctaagcctg ccaccgttga tgaatctgcc
     9181 ggacttccca aaaatccaga tcggtctcaa gtggccgtaa acagaaagcg cgaaggaaga
     9241 agtttcccct cgcgcctgcc aaacccgatc gattgagctg ccgatcgaac gaactactcc
     9301 tgaggtctaa caggcacata aacgggatcg acttcaccca cccatcgggt tgaggtctgc
     9361 ccgcctattc gattaaagcg taagggtaac gctaagagtt tcatcgacgc taagattaca
     9421 cgaaaattta aatttggtca agatttttgg tgacaacaaa tttgcccacc gcgccatcct
     9481 cggcacggtg ggcggcttga gcatttgctg accgtgtttg acgcgcggac aatcacgcgg
     9541 tacactacgc caatatttta attttggaag aggagcaaca cgtggcacat tcactccaga
     9601 tttttatcga tgaatatgtt cagcagctcg agccgctgct caagctgcgc aacgcggcgt
     9661 attgggattt tacggcccaa ggcgatgagc aaattcagca ggaatatgcg cggttggata
     9721 cggaaatccg gcagattcat tccgataaag cacgctttga agaattgaaa aaattgtcgg
     9781 ccacacccag cggcgatgcg ctgctcgatc ggcaggcgcg gttaatgcgc gatagctttc
     9841 aaagccagca gatgtcgccg gatatgatca aacagatcgt tgcgctggag accgacatca
     9901 ccgccgagtt caacggcttt cgcgccgacc tcaacggcca atcgatgacg gacaatcagt
     9961 tgaaaaagat tctgaaagaa tccaacgatg aggtcgagcg gcgcgccgcc tgggaagcca
    10021 gcaaacagat cggggcgcag gtggccgatc gggtcaggca aaccgtgcag ctgcgtaatc
    10081 gcgccgcgcg tgaaatgggt ttcgccaatt attatgccat gagcttgcac ttgcaggaat
    10141 tgaatgaaga tacattgttc gctttgttcg atcgattgga aaaactcacg cggccgttgt
    10201 tcaccgctta caaaaccgat ctggat
//
LOCUS       DASRTJ010000016         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_303598,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000016 DASRTJ010000000
VERSION     DASRTJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303598"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VFF70_00300"
     CDS             complement(<1..1561)
                     /locus_tag="VFF70_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZY43162.1"
                     /translation="MKVDTILAHGTLIVMDDSFTLIKDGALAIAGDKIAAVGTSDRIL
                     AEHSSDRMIDCRDCAIMPGLINAHTHVPMTLLRGMADDLRLDVWLMGYMMPVERAFVS
                     PEFVRIGAMLGCAEMIRSGTTSFADMYYFEDAIAEAVAHAGMRGVLAQTVLKFPAPDA
                     ASYEDSIEACRKFIEKWKGHALIIPAVGPHAHYTCPSEVLEACVALATEYNVPLHTHV
                     SETAFEVEESRKQHGMPVVPWIKKHGLLETQLIAAHCVHVDNGEIRSLQRSGAGIAHN
                     PSSNLKLASGLAPIAEMIQQNVKLGIGTDGTASNNDLDMFEEMRLASFIAKGAAHDPL
                     VVPARTALMLATIGGARAIHIDHLAGSLEVGKRADVTVVDLTGLHNTPRFSHDPNAVY
                     SQLVYACKSTDVRDVWCNGRALMRDRELLTVDTEFVMEQAQEIAAKIDAFLTAREGNV
                     LNKLIAIGGLAQEESYEVQVKAAIESPDLVERALQHSGLQIIRSSVRKQYDTYFIFSR
                     AAEERIRYREDE"
     gene            1785..2672
                     /locus_tag="VFF70_00305"
     CDS             1785..2672
                     /locus_tag="VFF70_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZY43163.1"
                     /translation="MIAYVFSGGNIRGALQVGAAQALLADPSRRPDLIVGTSIGAING
                     THLAANPTLQGALHLAEIWRSIRRSDVYPGGRLRILWRLIRRRDSIFPNEPLRSMLMQ
                     TVTEAKTFGDLLIPMFITANTLNSNAVYLWGDDPSASIIEALLTSTAVPSIFPPMRYN
                     GYEYVDGGILTNLPLDVAVDRGATEIYALDVSYLNETLPSVNGAITMVQRTIQIMLHQ
                     QAVDQLQRVTQIPGVIVHHLQLPGYKEMAINDFTQSDAMIVAGRKLAEEYLASPRPNF
                     IHIQSAPPPPPGAVRFDRI"
     gene            complement(3181..>4266)
                     /locus_tag="VFF70_00310"
     CDS             complement(3181..>4266)
                     /locus_tag="VFF70_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43164.1"
                     /translation="VSFQITAARAYIKYIGGGYAPDVTNVAEDLHTADSAASALVSVG
                     GSISVRGAADAYGCGSEQISEYSLWVKGDPTFSLTQPANGTAYNPGAGGWSNITTVTY
                     TTNDQRNYNKLDGMPAPDFLTNGSVWSSRTICSYIDFLPPFCFPVPDLVEFYWGSGTS
                     GKYAFLLKVVDTGGNTYYDLQRAWIDNETIRGKIANLRYHGSLSDIPPCTDILVNDGS
                     NHARQLDIRGFATDPLIVTTDLTQPTSDNFSSYNLTFRKQSAVSEVAVLSSTNPVPNR
                     ATWTGGMGDPPTDLLALLNLSWIDAGTPAPNDANGNPVPADNRLARGTSCTYDIYLRV
                     YDKTIVNEGTDHNLLYTFPVKIVNDLT"
BASE COUNT          956 a         1226 c         1105 g          979 t
ORIGIN      
        1 gttcatcttc gcgatagcgg atgcgctctt cggccgcgcg gctgaagatg aaataagtgt
       61 cgtactgttt tcgcaccgac gatcgaatga tctgcaaacc gctgtgttgc agcgcacgct
      121 ccaccaaatc gggcgactcg atcgccgcct tgacctgcac ctcgtaactt tcttcctgcg
      181 ccaaaccgcc gatggcgatc agtttgttga ggacatttcc ttcgcgcgcc gttaagaacg
      241 catcgatctt ggcggcgatc tcctgcgctt gctccatcac aaactcagta tcgaccgtca
      301 gcaattcccg atcgcgcatc aacgcccggc cgttgcacca cacatcgcgg acatcagtgc
      361 ttttacaggc gtacaccagc tgtgaataaa ccgcgttggg atcatgcgag aagcgcggcg
      421 tgttgtgcag cccggtcaga tcgacgacgg ttacatcggc acgcttgcca acctccaacg
      481 aacccgccaa atgatcgatg tggattgcgc gcgcgccgcc gatggtcgcc agcatcaagg
      541 ccgtgcgcgc tggcacaacc agcggatcat gcgccgcgcc tttggcaatg aacgatgcca
      601 gtcgcatctc ttcaaacata tccagatcgt tgttactggc cgtgccatcc gtgccgatgc
      661 ccagcttcac attctgctgg atcatttcgg cgatcggcgc caggccgctg gctaatttca
      721 agttgctaga tggattgtgc gcaatcccgg cgcccgatcg ctgcagcgat cgaatctcgc
      781 cgttatcgac gtgcacacag tgcgccgcga tcaattgtgt ttccaataaa ccgtgcttct
      841 tgatccacgg cacgaccggc atgccatgct gcttgcggct ttcctctacc tcgaacgccg
      901 tttcagacac atgcgtatgt aaaggaacgt tgtattccgt cgccaacgcg acgcacgcct
      961 ccaacacttc cgacggacag gtgtaatgcg cgtgcggccc gacggctgga atgatcagcg
     1021 catggccttt ccatttttcg ataaacttgc gacaagcctc gatcgagtct tcatatgagg
     1081 cggcatccgg cgcagggaat ttcaacacgg tctgcgccaa cacaccgcgc atgcccgcat
     1141 gcgccaccgc ttcggcaatg gcatcttcga agtaatacat atccgcgaac gaagtggtgc
     1201 ccgatcggat catctcggca caaccgagca tcgcgccgat gcggacgaac tcggggctta
     1261 caaacgcccg ctcgaccggc atcatataac ccatcaacca cacgtccagt cgcaggtcat
     1321 cagccatgcc gcgcagcaac gtcatcggca catgcgtgtg cgcattgatc aagcccggca
     1381 taattgcgca gtcgcgacag tcgatcattc gatcggacga gtgctcggcc aaaatccgat
     1441 cggatgttcc gacggctgcg atcttatcac ccgcgatcgc caaggcgcca tctttgatca
     1501 atgtgaatga atcatccatc acgatcaatg tgccatgcgc caaaatcgta tcgactttca
     1561 tactctgctc cgttcaatca aattcaagtg attatagcac gccgatcgta ataatttttt
     1621 atgggcgcgc gcgcaatcaa tggtcgatcg atccccaacg gcgcgccaaa caatctcgcc
     1681 atgtatttgc gtgattaagt tgcgattaat ccatttgcca gtataatccg ccaaccatct
     1741 tcgtctgtca tgtgtcattt ttcattgtgc atggcttctt gcctatgatt gcttacgtct
     1801 tctccggtgg aaatattcgc ggtgcactgc aagtcggcgc ggcgcaagcc ctcttagccg
     1861 atccatctcg ccgtcccgat ctgatcgtcg gcacatcgat cggcgcgatc aacggcacac
     1921 acctcgccgc caatcccacc ttacaaggtg cgctgcatct ggccgaaatt tggcgcagca
     1981 ttcggcgctc agatgtttat cccggcggtc gattgcgaat cctgtggcgc ttgattcgcc
     2041 gccgagatag tatatttcca aacgagccgc tgcgatcaat gttgatgcaa acggtcaccg
     2101 aagccaaaac ttttggcgat ctcttaattc cgatgttcat caccgccaac acactaaaca
     2161 gcaatgcggt ctatttgtgg ggcgatgatc cgtccgcatc gatcatcgag gcactgttga
     2221 cttcaaccgc ggtgccatcg atctttccgc caatgcgcta caacggctac gagtatgtcg
     2281 atggcggaat tctcaccaat ttgccgctcg atgttgcggt cgatcgtggc gcgacagaaa
     2341 tctacgcgct ggatgtcagt tacttgaacg agacgctgcc atcggtcaac ggcgcgatca
     2401 cgatggtgca gcgcacgatc caaatcatgc tgcaccaaca ggccgtcgat caactccagc
     2461 gcgtgacgca gatacccggc gttattgttc atcacctgca gctgccgggg tacaaagaaa
     2521 tggcgatcaa cgacttcacg caatcggacg cgatgatcgt tgccggacga aagttggccg
     2581 aggaatattt ggccagccca cgaccgaact tcattcatat acaatcggct ccgccaccgc
     2641 caccgggagc ggtgagattc gaccgaatat aaaggaggcc ggatcttaca atgagaaccg
     2701 gcctccttta ttgtgaatat cttaccccac cttcggcaat ttctgttttc ggatttcggc
     2761 atgcatccgc aatccactgc tgccaaacag cgtccatgct gttgggaggt gttaatgaaa
     2821 aaatttgtgt ggagatgtca aaagtcaata aggtggcagc catggattat tcaaagaacg
     2881 tatcgcttca ttgcggctga acctggtgtg atactgattg aaccaaacaa aagtgatcat
     2941 tcgaatgcct cctcgattag catctcaaat tttgatgtcg gcgcgaacgc gtatacgata
     3001 aaacaaaaag cctcgaggtt tgaaccccga ggcttttcat ttctacagat tgcgcttatg
     3061 tataccgcat aacgcaacac tttcgttttc ccaaaccact ggcaattcgc tctgcgtggg
     3121 gagcagtggc cacgttttgt tgcgaatcgt ttacactgtc atgattggaa cgatagcaca
     3181 ctaggtgaga tcgttgacga tcttgacagg gaaagtatag agcagattgt ggtcggttcc
     3241 ctcgttgaca atcgtcttgt cgtaaaccct taagtagatg tcataggtgc aactcgtgcc
     3301 acgagcgaga cggttgtcgg ctggtaccgg attgccattc gcgtcgttcg gcgcaggcgt
     3361 acccgcgtca atccaactca agttcaataa cgcgagtaga tcggtgggcg gatctcccat
     3421 accgcccgtc catgtggcac gattcgggac cggatttgtc gaagaaagaa cggcaacttc
     3481 gctgaccgcg ctttgtttgc ggaaggtcag gttgtaactc gaaaaattgt cgctggtcgg
     3541 ctgcgtcaag tcggtcgtga cgattagcgg atcggtcgca aagccgcgaa tatccagttg
     3601 acgtgcatga ttgctcccat cattcaccag gatatccgtg cagggtggaa tatcgctcag
     3661 gctcccgtga tagcggagat tggcgatctt gccgcgaatc gtctcattat caatccacgc
     3721 ccgttgaagg tcatagtatg tgttgcctcc cgtatccacg accttgagca ggaacgcata
     3781 cttgccgctt gtgccactgc cccagtagaa ctcgaccaga tcgggtacgg ggaagcagaa
     3841 cggcggcaga aaatcaatgt aagaacagat agtgcgcgac gaccagacgg acccattcgt
     3901 caaaaagtcg ggggcgggca tgccgtcgag tttattgtaa ttgcgctggt cgttagtggt
     3961 ataggtcacc gtcgtaatgt ttgaccagcc acctgcgcct ggattatacg cagtgccatt
     4021 cgcgggttgg gtgaggctga aggttgggtc gcccttcacc cagaggctgt attcgctaat
     4081 ctgttccgag ccacaaccgt aggcgtccgc tgcgccccgc acactgatag acccgccaac
     4141 actgaccaac gccgacgccg cgctgtccgc ggtgtggaga tcctcggcga cgttcgtcac
     4201 gtcgggcgca tagccaccgc cgatgtactt gatgtacgcg cgcgccgcgg tgatctggaa
     4261 tgagac
//
LOCUS       DASRTJ010000017         7748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_943,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000017 DASRTJ010000000
VERSION     DASRTJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..537)
                     /gene="greA"
                     /locus_tag="VFF70_00315"
     CDS             complement(67..537)
                     /gene="greA"
                     /locus_tag="VFF70_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZY43165.1"
                     /translation="MAEPATFLTKEGLKKLEEELHHLQTVRRQEVADRLHAAIEEGDI
                     DENAEYDDAKNEQAFVEGRIMTLENLLKNAVLIDESAKVSDEVTLGCKVTIVEGNSAP
                     EVYQLVGAAEADPTKDKISNESPLGKALIGRRVGDTVQVNAPAGLLTFRIASVS"
     gene            705..968
                     /locus_tag="VFF70_00320"
     CDS             705..968
                     /locus_tag="VFF70_00320"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZY43166.1"
                     /translation="MRIIVLAGPYSLGAIPCAVLVARSRGVDIRSVGSGNPGAHNVMH
                     QVGQFWGWQRVVSCITGSNQIDSNTRQYRASNTIMAWGKGVIR"
     gene            1028..2029
                     /locus_tag="VFF70_00325"
     CDS             1028..2029
                     /locus_tag="VFF70_00325"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006006904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HZY43167.1"
                     /translation="MSSRQFTMQIDQLPRRSLGFFPTPLHPLPNLSKRLGGPQLWVKR
                     DDQTGLATGGNKTRKLEFLLGDALQQNADLLMTVGAPQSNHARQTAAAAAQAGLRSTL
                     VLRGTPQTDRGGNILIDDLIGAEVRWAGDRPPQDLMNTIAEVMKLKGHRPYIIPLGGS
                     NAVGAVGYVTAMQELLDQAQALNVTFDAIVFATSSGGTQAGLVVGARRAGYTGRILGI
                     SVDHHTAGLTPVLIEIANATANRIDLAFTFSASDFEVNDQYLGGGYAILGDPERKAIR
                     ITAQTEGLLVDPVYTGRAMAGLMDLIRRREFRLDQNVLFWHTGGTAALFAYSKQLEH"
     gene            complement(2147..2707)
                     /locus_tag="VFF70_00330"
     CDS             complement(2147..2707)
                     /locus_tag="VFF70_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY43168.1"
                     /translation="MKITPRVVVVVAVLLMCVAAIVPAGSAAASNVFDSPVPTVAPTP
                     APGNLGVHTVRAGESLYCIGRAYGISPWAIASTNGVPWPYTIFVGQALNIPNVPWLNI
                     PAGPICVKQFGGGVPVPTPAPTSVPATPGPTPVPGTCVQSYLILPGDTLFGISIRFGV
                     DIPTLAARNHIANINLIFAYTTICIR"
     gene            complement(2816..5362)
                     /locus_tag="VFF70_00335"
     CDS             complement(2816..5362)
                     /locus_tag="VFF70_00335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZY43169.1"
                     /translation="MRLVRRILMGLVILIVLLAALVGGGYYYLSHRAFPQVDGTLQVS
                     GLTTPVSIIRDKSGIPHIYASNTHDLFFAQGYTEAQDRLWEMEVYRIGISGRTSELQP
                     SSSSLETDKFVLTLGFRRAAQADLAALDPKSLGYLKAFADGVNAFISTHQNTLPLEFT
                     IVGLFGSKGANYKPEPWSPVDSLEWAKAMAWNLGGNWQDELFNAQILAKFGDEPGKTI
                     IVDLKPAYDYKSHPIVVPSGVSWNNVPSSLANLQTVDSILGLRGRDIGSNNWTIAGSH
                     TTTGLPILANDPHLGIQIPSIWYFNGLHCKPVTTECPFEVIGATFPGVPGVVIGHNAK
                     IAWGLTNVGPDVQDLFLEKITGNQYEYRGQQVDLTIVPETIKITGQLPTDYTPSTNET
                     SVYDEAANLTTITLNVRVTRHGPIISDVDKDTAKLGYPVAFAWTAVNAPERTIDSVLG
                     VDTASNWNEFLSALAYFGSPSQNFVYADVDGNIGYHTPGRIPVRAKGDGQLPVPGWTG
                     EYEWTGYIPFSELPQSFNPDRGYILTANNAIVGPDYPYLISRDWDRGYRARRISDLLS
                     AKLKLSADDIAAIQVDDLNLSAQEIQPYLTSLTVDGDAAKVLDAIKTWDFKEQRASVG
                     ATAYEVFWYYLLRNTFDDELGDLASSYVDGGDVNREAMGRLLSKVDAPWWDNVTTSAK
                     EARDDILKKSLTDAAKALTTELGSDPAGWTWSKVHAAVFRSRALGTSPVAFIFNRGPI
                     PVDGGTAIVNNTGGNFSDGYSDPADPTAPSAKLTDIFAEQTVPSLRQIIDLSDLNKSI
                     FINTTGQSGLPTHPHYDDMIDPWRNIQYVPMWWAEQDIKSHAEGTLMLTP"
     gene            5505..7022
                     /locus_tag="VFF70_00340"
     CDS             5505..7022
                     /locus_tag="VFF70_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43170.1"
                     /translation="MANPITIDQSRSAQRATRLTPYFSLGTRYSPLFIIFTAFILRLG
                     SIAFQSLWRDEVDAIRFALAPLPDLIKTLTQPGYNGPLYFLMLRGWIGVAGQSEFAVR
                     FPSLVFGVISVVLIFALGKRLFNRSIGLIAATLFAFSAYQVWYSQEAKMYTLITALAL
                     ASVYFLRRGIEEGRTRYWTGLVVATSLAMYAHILAALLIPVEAALFILWWRMSRSQIR
                     SGAIALTLLTVPYIPLAGWELPLIFQPAETGFANYTFGGMLNTLGNAYTVSILTPLTS
                     WLISLVIGVSAALAIWGLLESKFSVTHRLALLLWLALPFLAIFVVSINRPIFTDRYLI
                     WIEAAYFLLISIGLYSLWRIWKPIGLAGLIGLVVVSLLSINAQATTPFKSDFRSGAHA
                     IEREIKPPEAIVFQIPYVQYTFDYYFHQRYRPIEGPYTNYPGNTNGYRDSEDTVIQSL
                     TTQFQNQQSVWLVLSEEEMWDRRHLLRQWLDSHGTVTYHAIFAQVEITRYQLSPP"
     gene            complement(7080..>7748)
                     /locus_tag="VFF70_00345"
     CDS             complement(7080..>7748)
                     /locus_tag="VFF70_00345"
                     /inference="COORDINATES: protein motif:HMM:NF037737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43171.1"
                     /translation="LHVVSVGESRIDMAIGDLEKLSNPSVGLSAKAGQIDIRIAAKAA
                     TRSGAKRLTAEIEQHVRERVGENIFGADEDTLEGVIAGLLADQHTTLASVELGTGGLI
                     GGRLSGAQNQSFRGGLVLSDYKALHRSLPNAQDDESNQSAVIEAARGIREVHKSDLGL
                     AAILREAANHQGLRLFVALVTQDNVETLLRGFGGHSGLAAQWASNIALGLVWKFLKAR
                     DVEE"
BASE COUNT         1784 a         2093 c         2018 g         1853 t
ORIGIN      
        1 gagcaggtta ctaaaaagtc catgcgatcg gttccgcatg gacttgatta aatcacgctt
       61 actcgatcaa ctaactgaag cgatgcggaa cgtaagcaga ccggcgggtg cgttgacttg
      121 aacggtatcg cccacgcggc ggccgattaa cgctttgccc agcggacttt cattggagat
      181 cttgtcctta gtcggatcgg cttcggccgc accgaccaat tgatacactt ctggcgcgct
      241 gttgccttcc acgatcgtga ctttgcagcc cagtgtcact tcatcgctga ccttcgcgct
      301 ctcatcgatc aagactgcat tcttgagcaa attctccagt gtcatgatgc ggccttcgac
      361 aaacgcttgc tcattcttgg cgtcgtcata ttcggcgttc tcatcgatgt cgccttcttc
      421 tatcgcggcg tgcaagcgat ctgctacttc ctggcggcga accgtttgca gatggtgtaa
      481 ttcttcttca agttttttta gtccctcttt ggtcaagaag gttgctggct cggccataca
      541 tttctcctgg aacagttgcg attgtcaata aaatgcgccc actcgcgcat catataaata
      601 aagactgaga gctgtcgcct ctcagtcccg ttcaaagtac tgccaacatt ataatgagtt
      661 tcgggcgatt tgtaaagcgg gatgtcgatg ctataatcgg gtcgatgagg atcatcgttc
      721 tggccggacc gtactcatta ggcgcgattc cctgcgcggt cctcgtcgcc cgatcgcgtg
      781 gagtggacat ccgatcggtc ggatcgggta atccaggcgc gcataatgtg atgcaccagg
      841 tcggccaatt ctggggctgg cagagggttg ttagctgcat taccgggagc aatcaaattg
      901 attcgaacac aaggcagtat cgggccagca atacgatcat ggcgtggggg aaaggagtga
      961 taagatgaat agatgattgg cgggcaagat gatctggtcg atagaatcat cttgtcagca
     1021 tgtcatcttg tcatcgcgtc aattcacgat gcaaatcgat caattgcctc gccgctcatt
     1081 aggtttcttc cccacaccgc tgcatccgct gccaaatcta tcgaagcgcc tgggcggacc
     1141 gcagctatgg gtcaaacgcg acgatcaaac cggtctggcg acgggcggca acaaaacacg
     1201 caaattggaa ttcttgttag gcgacgcatt gcagcaaaac gccgatctat tgatgaccgt
     1261 cggtgcgccg cagtcgaatc acgcgcgcca gactgcagcc gcggctgcac aagccggctt
     1321 gcgatcaacc ttagttctgc gtgggacgcc acaaactgat cgggggggca acattctgat
     1381 cgatgatctg atcggagcgg aagtgcgctg ggccggcgat cggccgccgc aggatttgat
     1441 gaatacgatc gccgaggtga tgaagctgaa aggtcatcga ccctatatta ttcccctcgg
     1501 cggctccaat gcggtcggtg cggtaggcta tgtgacggcg atgcaggagt tgctcgatca
     1561 agcgcaggcg ctgaatgtga cctttgatgc gatagttttt gcaacaagct ccggtggcac
     1621 tcaagcgggg ctagtcgtgg gcgcacgtcg agccgggtac accggtcgga ttttgggcat
     1681 cagtgttgat caccataccg ccgggttgac gccggtgttg atcgagatcg cgaatgcaac
     1741 agctaatcga atcgacctgg cctttacttt cagcgcgagt gattttgaag tcaacgatca
     1801 gtatttgggt ggggggtatg ccatattggg tgatccggaa cgcaaagcga ttcggatcac
     1861 cgcccagact gaaggactct tggttgatcc ggtctacact ggacgggcga tggctggctt
     1921 gatggacttg attcggcggc gcgagttccg actcgatcaa aatgttttat tctggcacac
     1981 gggcgggaca gctgccttgt ttgcgtactc gaagcagctg gaacactgac ccttgcaata
     2041 gtttcaaaac ttattgcccg ctccaaacaa aactttcgaa agattcaata tcaccaaaaa
     2101 gaggcaacct cttcacagaa gttgcctctc tgcattttgg tccagtttat ctgatacaga
     2161 tcgtcgtgta ggcgaaaatc aagttgatgt tcgcgatgtg attacgggcc gccaacgtgg
     2221 gaatatcgac gccgaaccga atagaaatac caaacaaggt atcgccgggc aggatcagat
     2281 acgactgaac gcatgtgcca ggcacaggcg ttggtccggg tgtagccggg acagacgtcg
     2341 gcgccggagt cggcacaggc acaccgccac caaactgttt gacgcagatc gggccggccg
     2401 gaatattcag ccacggcaca tttggaatat tcagtgcctg acccacgaaa atcgtgtacg
     2461 gccacggcac gccattcgtc gaggcgatgg cccacggcga gatgccatac gcgcgaccga
     2521 tgcaatacaa cgactcgccg gctctcacgg tatgcacgcc taaattacct ggcgctggcg
     2581 tgggtgccac cgtcggcact gggctgtcaa aaacattcga cgcggcggca ctcccggccg
     2641 gaacgattgc cgcgacgcac atcaataata cggctacaac cacaacaaca cgcggagtaa
     2701 ttttcatgat aggagctcct tcgctaggat tgaatcacat tatagcgctg atgtccatta
     2761 tattcaaacg cgttgaaagc gcgcataccg attaagttac agttcgatta gcgccttagg
     2821 gcgtcaacat gagagtgcct tcggcgtggg atttgatgtc ctgttcggcc caccacatgg
     2881 gaacatactg aatgttgcgc cacggatcaa tcatgtcatc gtaatgcgga tgggtaggca
     2941 agccgctttg gccggtcgtg ttaatgaaga tcgatttgtt gagatcgctc agatcgataa
     3001 tctgtcgcag tgacggcacg gtttgctcag caaaaatgtc ggtgagttta gccgatggcg
     3061 cagtgggatc agcgggatca gaataaccat cgctaaagtt accgcccgta ttgttcacaa
     3121 tcgccgtgcc gccatcgact ggaatcgggc cgcgattaaa aatgaaggca accgggctgg
     3181 tgcctaaagc gcggctgcga aaaactgcag catgcacttt gctccacgtc caacccgctg
     3241 gatccgatcc gagctcggtc gtcaacgctt tggccgcatc ggtgagcgac tttttcaaaa
     3301 tatcatcgcg ggcttctttt gcagacgtag tgacattgtc ccaccacggc gcgtcaactt
     3361 tgcttaatag tctgcccatc gcttcacgat tcacatcacc gccatcgacg tacgagctcg
     3421 ccagatcgcc cagctcgtca tcgaaagtgt tgcgcagcag gtaataccag aacacttcat
     3481 acgccgtcgc gccgacgcta gcgcgctgct ctttgaaatc ccaagtcttg atcgcatcca
     3541 aaactttagc tgcatcgcca tcgaccgtga gcgatgttag atatggttgg atctcctgag
     3601 ccgatagatt cagatcgtcc acttgaatcg ccgcgatgtc atcggcgctg agtttgagct
     3661 tggcggaaag tagatcggag atgcgccgcg cgcgataacc gcgatcccaa tcccgcgaga
     3721 tgagatatgg ataatcgggg ccaacgattg cattatttgc cgtgaggatg taaccacgat
     3781 ctggattgaa tgactgtggc agttcgctga atggaatgta gcccgtccat tcgtattcgc
     3841 cggtccagcc cggcaccggc aattgaccat cacctttcgc ccgaacggga atccggccgg
     3901 gcgtatgata accgatgttg ccatcgacat cggcataaac aaaattttgg ctgggcgatc
     3961 cgaagtacgc cagcgccgaa agaaattcat tccaattact agcggtatcg acgcctaaca
     4021 cagaatcgat cgttcgttcg ggcgcgttga ccgctgtcca ggcaaacgca acgggataac
     4081 ccaacttcgc ggtatctttg tccacatcgc tgatgatcgg gccgtgccgt gtgacgcgca
     4141 cgttcaaagt gatcgtcgtc aagttcgctg cttcatcgta cacggaagtt tcgttcgtcg
     4201 atggcgtgta atcggtgggc agctggcctg taatcttgat cgtctctggc acgatcgtga
     4261 gatcgacttg ctgacctcta tattcatatt gattgccggt gattttttcc aagaacaggt
     4321 cctgcacatc cggcccgaca ttcgtcaagc cccaggcaat cttcgcgttg tgaccgatga
     4381 ccacaccggg cacgccgggg aatgtcgcac cgatcacttc gaaaggacat tcagtggtga
     4441 cgggcttaca gtgcaagccg ttgaaatacc agatcgatgg aatctgaatg ccgaggtgcg
     4501 gatcgtttgc cagtatgggt agaccggtgg tggtgtgact gcccgcgatc gtccagttgt
     4561 tcgatccgat gtcccgcccg cgaagcccca ggatcgaatc gacggtttgc aagttagcta
     4621 aactcgatgg gacattgttc cagctcactc cggacggaac aacgatcgga tgacttttat
     4681 aatcataagc gggtttcaga tcgacaataa tcgttttgcc tggctcgtcg ccgaacttcg
     4741 ccaggatttg agcattgaac aattcatctt gccagttgcc gcctaaattc caagccatgg
     4801 ccttggccca ttccagcgaa tcgactggcg accatggctc aggtttatag tttgcacctt
     4861 tgctgccgaa caatccgacg atcgtgaatt caagcggcaa ggtgttttga tgtgttgaga
     4921 taaaagcgtt cacgccgtcg gcaaaggctt ttaagtaacc gaggcttttg ggatcgaggg
     4981 ctgcaaggtc ggcttgggct gcgcgtcgaa acccgagggt cagcacaaac ttatcggttt
     5041 ccagactgga cgatgaaggt tggagctcgg acgtgcggcc ggatattcca atgcgatata
     5101 cttccatttc ccacaatcga tcttgtgctt cagtgtaacc ttgcgcaaag aacagatcgt
     5161 gcgtgttact ggcataaata tgaggaatgc cggatttgtc acgaatgatc gagaccggcg
     5221 tcgtcaagcc ggagacttgc aaagtgccgt ccacttgcgg aaaagcgcga tgagataagt
     5281 aatagtaacc accgccgact aacgctgcga gcaggacaat gagaatgacc agacccatca
     5341 gaatccgacg cacaagtcgc atacgtaacc tccggcttaa attggactca tttgtatcat
     5401 acctgaagca caaagtcaaa agatcggcgc gctcaacggt gcggtataat cgcttatcgc
     5461 atatcaaagg tgatgaataa cagatagcaa atggcgaatt gtaaatggca aatcccataa
     5521 cgatcgatca atcacgcagc gcgcagcgcg caacacggct gacaccgtac ttctcactcg
     5581 ggactcgtta ctcacccctc ttcattatct tcaccgcgtt tatcttgcgc ctcggctcga
     5641 tcgcgtttca aagcctctgg cgcgatgaag tcgatgccat acgttttgca ttggcgccac
     5701 tacctgatct gatcaaaacc ttaacgcagc caggctataa cggtccgctg tacttcttga
     5761 tgctgcgcgg ctggatcggc gttgcgggcc aaagcgaatt cgctgttcga tttccttcat
     5821 tagttttcgg cgtcatcagc gttgtattaa tctttgcgtt gggcaaacgc ctgttcaatc
     5881 gatcgatcgg tctcatcgcc gcaaccttgt tcgccttctc tgcgtatcaa gtgtggtata
     5941 gccaggaagc caaaatgtat accttgatca ccgcgcttgc actcgcctcg gtttattttt
     6001 tgcggagagg aatagaagag ggtcggaccc gctactggac cggtctggtc gtcgcaactt
     6061 cgttggccat gtacgcgcat atattggccg cgttgttgat tccagttgaa gcagcgttat
     6121 tcattctatg gtggcgcatg agccgcagtc aaattcgttc gggcgcgatc gcgctcacct
     6181 tgttgactgt gccttacatt cctttggccg gctgggaact gccactgatc tttcagccag
     6241 ccgaaaccgg ctttgcaaat tacacgttcg gtggaatgtt aaacacactc ggcaatgcct
     6301 atacggtaag catcttgacg cccttaacca gttggctaat cagcttggtt attggggtca
     6361 gtgcggcgct ggccatctgg ggtttgctcg aatcaaaatt ttcggtcaca catcggctcg
     6421 ctttgctgct ctggctcgcg ctgcctttct tagcgatctt cgtggtctcg atcaaccggc
     6481 cgatctttac ggatcgatac ttgatctgga tcgaagccgc ttactttttg ttgatctcga
     6541 ttggcttgta ctcattatgg cggatctgga aaccgatcgg tttagccggg ctgatcggct
     6601 tagtcgtcgt cagcctactc agtattaacg cgcaagcgac cacgccgttc aaatcagatt
     6661 ttcgatcagg agctcatgcc atcgagcgtg agatcaaacc ccctgaagcg atcgtctttc
     6721 aaatcccata cgtgcagtac actttcgatt attactttca tcaacgctat cggccgatcg
     6781 aagggccgta cacaaattat cccggcaaca ccaatggtta tcgcgacagt gaagataccg
     6841 taatccaaag cctgaccacc caatttcaaa atcaacagag tgtgtggtta gttttatccg
     6901 aagaagagat gtgggatcga cgacatctct tgcgccaatg gttggattcg catggcacgg
     6961 ttacttatca cgccatcttc gcgcaagttg aaattacgcg ctatcaactc agtccacctt
     7021 aaaacaaaaa gagccacact ctgcgactct ttcaatctgc aacttgccac ttgcacactc
     7081 tattcctcaa catcccgcgc tttcaaaaac ttccacacta aacctagagc gatattcgac
     7141 gcccattgag cagccaagcc cgaatgtccg ccgaagccgc gcagcagagt ttctacattg
     7201 tcttgggtca ctaacgcaac aaatagcctc aagccttgat ggttcgccgc ttcacgcagg
     7261 atcgccgcca aacctaaatc gctcttatgc acttcgcgga tgccgcgggc cgcttcgatg
     7321 accgcacttt ggttcgattc atcatcttgc gcattcggca acgatcgatg cagcgccttg
     7381 taatccgaca gcaccaaccc gccgcgaaac gattgattct gcgcgcccga caatcgacca
     7441 ccgatcaagc cgccggtgcc cagctcgaca ctggcgagtg tggtatgttg atcggctaac
     7501 agtccggcaa tgacaccttc cagcgtatcc tcatcggcac caaatatatt ttcgccgact
     7561 cgctcgcgca catgttgctc gatctcggca gtcaggcgct tcgctccaga gcgtgtcgcg
     7621 gccttagctg caattcgaat atcgatctgg ccggctttcg ccgacagacc gacactgggg
     7681 ttcgacaact tttccagatc cccaatggcc atatcaatgc ggctttcgcc cacactcacc
     7741 acatgcag
//
LOCUS       DASRTJ010000018         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_113956,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000018 DASRTJ010000000
VERSION     DASRTJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113956"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFF70_00350"
     CDS             complement(<1..1004)
                     /locus_tag="VFF70_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43172.1"
                     /translation="MSNPPATILCVDDDLLILELLDRIFAKYSYTILHAADSTAALQL
                     IHDRSDIDLILLDIMLPGGLDGVEICRRIRAVQTGAYVPIIMLTALGQTEHIATGLDA
                     GADDYITKPFSPREILARVQAALRLGRTQRELTEAQNRYRVLVETSRDVIFALNTAGQ
                     LSYISPACRTLTGYTAAELLADPQPLARVVRSEDAQRVAAYFQQASTTSEPAELEFQI
                     ISQDGHSRWVTLSCAAIHDAQGSISGIQGAARDITQRKQIEAATWQRSQELSALNLIA
                     TRVNQSLELENTLGETLDALMEVLGIEFGTIHVVQDGEFNLRAAHGVPDDVARTWHQL
                     L"
     gene            complement(1175..>2227)
                     /locus_tag="VFF70_00355"
     CDS             complement(1175..>2227)
                     /locus_tag="VFF70_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZY43173.1"
                     /translation="HLGLAIAAIWRGHQAILAPSPDQVVQANDILLIVGREERVAQLA
                     EQGCQIGREGTNGRLNGHISAQGVSFIEVMPAPHSQALSQTLKQLEFRNKYGFTVVAM
                     WRGGRSYRTDVGDFSLTLGDALLVVGAHNRLKSLQNNPDFIVLEPDVSDQPVQRKRAA
                     FTIGVISIAIVASIAGFPIHLAMLAGAILVVLAGIITMEEAYRAIEWQAIFMIAGMYT
                     VSLAMVKTGLAGIIGAGMVNVVTPLGPIGLAAGAYLLSVVLTQFMGGQVTALVTGPIA
                     ISAALTLHVNPQAIAVATAIGCSASFFTPIAHPVNVLMIGPANYQFRDFFRIGWRLTL
                     LCFVMLLIGMLLFWKL"
BASE COUNT          495 a          627 c          640 g          465 t
ORIGIN      
        1 agcagttggt gccaagtgcg ggccacatcg tccggcacgc cgtgtgccgc gcgtagattg
       61 aactcgccgt cttgcacgac gtgaatcgtg ccaaactcga tgccgagcac ttccatcaaa
      121 gcgtccagtg tttcacctag cgtgttttcc aattccagcg attgattgac tcgcgtagcg
      181 atcaagttca gcgccgagag ttcttgtgag cgctgccagg tggccgcttc gatctgtttg
      241 cgttgggtga tgtcgcgcgc ggcgccttga atgccgctga tcgatccctg cgcatcgtga
      301 atcgctgcgc aggaaagcgt gacccagcgg ctgtgtccgt cttggctgat gatctggaat
      361 tcgagttcgg ccggttcgct ggtggtggac gcttgttgaa aataggccgc gacgcgctgc
      421 gcatcttcgg aacgcacaac gcgcgctaaa ggctgcggat cggccagtaa ttctgcggcg
      481 gtgtagccgg tcaatgttct gcaggcggga ctaatgtagg aaagttgacc ggcggtgttc
      541 agcgcgaaga tcacatcacg cgaagtttcg accaggacgc gataccgatt ttgcgcttca
      601 gtcagttcgc gctgagtgcg gcccaggcgc agcgcggctt gcactcgcgc taaaatttcg
      661 cgcgggctga agggcttggt gatgtaatca tcagcgcccg catccagacc ggtggcaatg
      721 tgttcggtct gaccgagcgc ggtcaacatg atgatcggca catacgctcc cgtttgaacg
      781 gcgcggatgc ggcggcaaat ctcgacgccg tccagaccgc cgggcagcat gatgtccaat
      841 aagatcagat cgatatctga tcgatcgtgg attagttgca gcgcagcagt gctgtcagcc
      901 gcgtgcagta tggtatacga atatttggcg aagattcgat ccagcaattc caggatcagc
      961 aaatcatcat cgacgcatag aatcgtggca ggtggattgc tcatgacggg ttgagaggat
     1021 tatagcattg aacattggcg ggtgaaatgt agtaattgat gaatctatac gacattcctt
     1081 ggttactgtc acacccgcga aagcaggtgt ccaggttatc gttctggatt cccgcctgcg
     1141 cgggaatgac ggtaagcgta tgcagattcc tttgttacaa cttccaaaac aataacatgc
     1201 cgatcaacaa catcacaaag caaagcagcg tcagccgcca accgatccga aagaagtcgc
     1261 ggaactgata gttcgccgga ccgatcatca acacattgac gggatgagcg atcggtgtga
     1321 agaacgaggc cgaacagccg attgcggtgg cgaccgcgat cgcttgcgga ttgacatgca
     1381 gcgtcaacgc tgcgctgatc gcgatcggac cggtgactaa cgcagtaact tgtccaccca
     1441 tgaactgcgt cagcacaacg ctgagcagat acgctccggc cgccaaaccg atcgggccga
     1501 gcggcgtgac cacgttgacc attcccgcgc cgatgatacc cgccagtcca gttttcacca
     1561 tcgccagact gaccgtgtac attccggcga tcatgaagat tgcctgccac tcgatggcgc
     1621 gataggcttc ttccatggta atgatgcccg ccaagacgac gaggatcgct ccggccaaca
     1681 tggcgagatg aatcggaaag ccggcgatcg aggcaacgat cgcgatcgag attactccga
     1741 tcgtgaaagc ggcgcgcttg cgctgaaccg gttgatcgct gacatccggt tcgagcacga
     1801 tgaagtccgg attgttttgc agactcttca atcgattgtg cgcaccgacg accaacagtg
     1861 catcacccaa cgtcaagctg aagtcgccga catcggtgcg atagctgcgc ccgccgcgcc
     1921 acatcgccac aacggtaaag ccatacttat tgcgaaactc caactgcttc aaagtttggc
     1981 tcagtgcttg cgaatgcggc gcaggcatca cttcgatgaa cgacacgccc tgcgcgctga
     2041 tgtgtccgtt aagacgaccg ttagtgcctt cacgtccgat ctggcagcct tgctcagcca
     2101 actgcgccac gcgctcttca cgcccgacga tcaacaagat gtcgttggct tgcaccactt
     2161 gatccggcga cggggccaag atcgcttgat ggccacgcca gatcgcggcg atggctaacc
     2221 ccaaatg
//
LOCUS       DASRTJ010000019         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_38908,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000019 DASRTJ010000000
VERSION     DASRTJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38908"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..964
                     /gene="lgt"
                     /locus_tag="VFF70_00360"
     CDS             98..964
                     /gene="lgt"
                     /locus_tag="VFF70_00360"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZY43174.1"
                     /translation="MSCVLTHPGFCIGPIPVNWYGIIIVTGILMGGFVAAREARRKGE
                     NTDRVWDALMIVAIAGIIGARLYHVLSNPAGCNETIQGCGWPWYSQHPADIVLLMTQG
                     GLGIFGALIAGGLAAFIYIRRKKLSFARYLDIAAPAILIGQAIGRWGNFVNQELYGPP
                     TTLPWGISIDAAHRYGAFTDLTQYPVETTRFHPDFLYESILNTIGFIVLVIVARKFAN
                     RLKDGDLFLLYMIWYPVVRILVESLRPDAWTVAGGGLATAQLVSMVSIVIAVALLIFR
                     HRPHTPIEPASA"
     gene            1066..2103
                     /locus_tag="VFF70_00365"
     CDS             1066..2103
                     /locus_tag="VFF70_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY43175.1"
                     /translation="MQPVTRRQFLIAAGGSAIAAVIASCAPQLTSNNAATPSSGSAPT
                     AVPQVIEPPKKIYLSSIEGLYQQNYASTPDVDEKSWFMQIDGLVDSPLKLDMTEIKSL
                     PKYDDVRTLECIGNPVGGNLIGNVEWNGIRLKDLLAKVKIDPKATHVNFQAADGYYTS
                     VELDWLMQDRTLLAYGLNGAPLTPDHGFPLRINMPGLYGQKMPKWITHMEFTNNPVTG
                     FWESQGWSNTADVQTNSKFSEPHNGQHLEPGQNPVFGVAFAGKNKITKVEVSADGQNW
                     INCDLLQGDSPLVWTQWSGVWEAQPGKYEMEVRATDETGFTQNIPSGGYLAQSYPKGT
                     SAIATVAVRVE"
     gene            2112..>2719
                     /locus_tag="VFF70_00370"
     CDS             2112..>2719
                     /locus_tag="VFF70_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43176.1"
                     /translation="MRFKNGKRRWILTALFGAVLVLGAGGAIGGTLLEDHDDFCISCH
                     TTPEQTYYDRVQQALKDKAGVTDLKNLAAQRVAIDLASQHYIVTDTLSCISCHGGVRN
                     LADRVESLALGAKDSLVYFLGRADQTIEKTTTGDPALINRACVGCHATQLVTVKFENH
                     FHVKLPQAWNLFQGGAQPIVTADNPKALTDPRSKPELLDTSIT"
BASE COUNT          611 a          730 c          760 g          618 t
ORIGIN      
        1 cctcggtgtc ctgtttgtcc tcagaagttt cctgtaaata aacagccttg agtgatgtat
       61 aatcgcgctg tgatgaattc ccgccaagga gagcaatatg tcgtgtgtgt taacccatcc
      121 cggattctgc attggtccaa taccggtgaa ttggtatggc atcatcatcg tcactggaat
      181 attgatgggc ggctttgtcg ctgcacgtga agcgcgccgt aaaggcgaga acaccgatcg
      241 ggtgtgggac gcgttaatga tcgtggccat tgcgggcatt atcggagcac ggttgtatca
      301 tgtactctcc aatccagccg gttgcaatga aacgattcaa ggctgcggtt ggccatggta
      361 cagccagcat ccggccgata ttgtattgtt gatgacacag ggcgggctgg gaattttcgg
      421 cgcgttgatc gctggcgggc tggccgcctt catttacatt cgtcgtaaaa aattatcttt
      481 tgcgcgttac ttggatattg ccgcgcccgc aatcttgatc ggtcaagcga tcgggcgctg
      541 gggcaacttt gtcaatcaag aactgtacgg cccgccgacg acgctgccgt ggggcatttc
      601 gatcgacgcg gcgcaccgct acggcgcatt tacagatctc actcaatacc ccgttgagac
      661 gacgcgcttc catcccgatt ttttatacga gtcgattttg aacaccattg gctttattgt
      721 cttagtgatc gtcgcgcgca agtttgccaa tcggctgaaa gacggcgatc tgtttttgtt
      781 gtatatgatc tggtatcccg tggtgcggat tttagtcgaa tcgctgcggc ctgatgcgtg
      841 gaccgttgcc ggcgggggct tggccacggc gcagctggtc tcgatggtct cgatcgtgat
      901 tgcggtggca ttattgatct ttcgccatcg gccgcacacg ccgatcgagc cggccagcgc
      961 ttagccgcgc aagatgttcg taataaattc cccggacaat agactgtata gcattggcgt
     1021 aacagcacct gttgtgccaa ttaactttag ccaaggtagg aaagtatgca accggttacc
     1081 cgtcgacaat ttctcatcgc cgcgggcggc agcgccatcg ccgcggtgat agccagctgc
     1141 gcgccgcagt tgacttccaa caatgcggca acgccgtcat ccggcagcgc gccgaccgcc
     1201 gtcccgcaag tgatcgaacc tcccaagaaa atctatctat ctagcattga aggtctgtat
     1261 caacagaact atgcttcgac gccggatgtc gatgaaaaaa gttggttcat gcagatcgat
     1321 ggtctggtcg attcaccgct gaagcttgat atgaccgaga tcaaatcact gccgaagtat
     1381 gacgacgttc gcacactgga atgcatcggc aatccggtgg gcggcaactt gatcggcaat
     1441 gtggagtgga acggcattcg gctgaaagat ctgttggcga aagtaaagat cgatccgaag
     1501 gcgacgcatg tgaactttca agccgccgac ggttattaca ccagcgtcga actcgattgg
     1561 ctcatgcagg accgcaccct gctggcttat ggtttgaacg gagcaccctt gacgcccgat
     1621 catggtttcc cgctgcgcat caatatgccc ggcttatatg gccaaaagat gcccaagtgg
     1681 atcacccata tggaattcac caataacccg gtgaccggct tttgggaaag tcagggttgg
     1741 tcgaacacgg ccgatgtgca aacgaattcg aagttctccg agccgcacaa cggccagcat
     1801 cttgagcccg gccagaatcc ggttttcggt gtggcctttg ccggcaagaa taaaatcacc
     1861 aaagttgaag tgtcagccga tgggcaaaat tggatcaact gcgatctcct gcaaggcgac
     1921 agcccgctag tctggacgca gtggtccgga gtctgggagg cccagcccgg caagtacgaa
     1981 atggaagtgc gcgcgaccga tgaaaccggc ttcacccaaa acattccgag cggcggctat
     2041 ttggcccaat catatcccaa gggcaccagc gccatcgcga cggtggctgt gcgcgtggaa
     2101 taggctcgtt catgcgattc aagaacggga agcgacgctg gattttgact gcgctgtttg
     2161 gcgcagtcct cgttttgggc gcgggcggcg cgatcggcgg cacgctgctg gaagatcacg
     2221 acgatttttg catcagctgc cacaccacgc ctgaacaaac ttattacgat cgagtccagc
     2281 aagcgctgaa agacaaagcg ggcgtgactg atctcaaaaa tttggccgcg cagcgcgtgg
     2341 cgatcgatct cgccagccag cattacatcg ttaccgatac gctgagctgc attagctgcc
     2401 atggcggcgt gcgcaatctg gccgatcgag ttgaaagtct ggcgcttggc gcaaaagaca
     2461 gcctcgtgta ttttctcggt cgcgccgatc aaacgatcga gaaaacaacg accggcgatc
     2521 cggctttgat caatcgcgcg tgtgtcggct gtcacgccac gcaactggtc actgtcaaat
     2581 tcgaaaatca tttccacgtc aaactgccgc aggcctggaa cttatttcag ggtggggctc
     2641 aaccgatcgt gaccgccgac aacccaaagg ctttgactga tcctcgatcg aaaccggaac
     2701 tgttggatac ctccatcac
//
LOCUS       DASRTJ010000020         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_228121,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000020 DASRTJ010000000
VERSION     DASRTJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228121"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFF70_00375"
     CDS             <1..833
                     /locus_tag="VFF70_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZY43177.1"
                     /translation="IVNADDYGLTAGVSAGIRRAHSNGILTSTTALMNRPNVEAELKI
                     AAAESPALGIGVHLNLTSGDPVLSPAQVPTLMKVFDQHDHGLQRLDLIGQLNMAEVQA
                     EWRAQIEKFVRASGQPPDHLDSHHHSSYYTPELCSLMLDLAREYHCAIRLPFAHLDEP
                     NFIFDDLPIDLRDRAKQEVSKLLIDAIDVPKPDHFFADFYNETATLKTLLELLAALPD
                     GISELMTHPGYNDATLSGFSIYNEPRSRELAVLTDPAVRNYLAVNKIELISFANLKHA
                     "
     gene            853..>1624
                     /locus_tag="VFF70_00380"
     CDS             853..>1624
                     /locus_tag="VFF70_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZY43178.1"
                     /translation="MTSYYLGADVGGTNTRILIAAEDGHAIGYGQSGPGNHEQVGYDG
                     FTRAVQAAAQHALTVANITINQIDHAAFGIAGYDWPAERAETLGALQSIGLSCPIEIV
                     NDATIGLVAGSEAGWGIAIVSGTGCNCHGLDQTRRREGQVTGGGITLGEAAGSSELVA
                     KAIQAVAYEWTRRGPATQLTALLLKHTGTHTVESFLDRMMNGRLNLDAAAAPLVFQAA
                     HNGDEVALNLIRWAGHELAELAKAVIRQLDLAPLAFDVV"
BASE COUNT          375 a          473 c          428 g          348 t
ORIGIN      
        1 tgattgtcaa cgcagatgat tacggcctaa cagccggtgt gtccgccggt atccgacgcg
       61 ctcattcgaa tggaatctta acttccacca ccgccttaat gaacaggccg aatgtcgagg
      121 ccgaattgaa aatcgccgcg gcggaaagtc ccgcgctcgg aattggggtc caccttaatt
      181 tgacgtcagg cgatccggtt ttgtcacccg cgcaagtgcc gacgctaatg aaggtctttg
      241 atcaacacga tcacggactg cagcgcttgg atctgatcgg acaactcaat atggcagaag
      301 tacaagctga atggcgcgcc cagatcgaga aatttgtacg cgcgagcggc cagccacccg
      361 atcaccttga tagtcatcat cacagttcat attacacacc cgaactgtgc agcctgatgc
      421 ttgacctcgc gcgcgaatat cactgcgcga tccgtttgcc cttcgcccat cttgatgaac
      481 cgaatttcat cttcgacgat ctcccgatcg atctgcgcga ccgggccaaa caagaagttt
      541 caaaattatt gatcgatgcg atcgatgtgc ctaagcccga tcacttcttc gccgacttct
      601 ataatgaaac cgccaccctt aagacgctgc tggagcttct cgctgctctg ccggatggca
      661 tatccgaact catgacgcat cctggttaca atgacgctac attatcaggc ttcagcattt
      721 acaacgaacc acgctcgcgt gaactcgctg tattaaccga tccggcggta cgaaattatc
      781 tggcagtgaa taagatcgaa ttgatctcgt ttgccaattt gaagcacgct taatttattg
      841 gagttaccta tcatgacctc gtattatctt ggtgcggatg ttggcggcac caacacacgc
      901 atcctcatcg ccgctgaaga cggtcacgcg atcggctacg gccaaagcgg gccaggcaat
      961 catgaacaag ttggctacga cggcttcacc cgcgccgtgc aagccgccgc gcaacatgca
     1021 ctaacggtag cgaatatcac gatcaatcaa atcgatcacg cggctttcgg catcgccggt
     1081 tatgattggc cggccgagcg cgccgaaacg ctgggcgcgc tccaatcgat cggcttgagc
     1141 tgcccgatcg agatcgtcaa cgatgcaacc atcggcttgg tcgccggatc ggaagcgggc
     1201 tggggcatcg ccatcgtatc gggcaccggc tgcaattgtc acggcctcga tcaaacccgt
     1261 cgacgcgaag ggcaagtcac cggcggcggc atcacgttgg gcgaagcggc cggtagcagc
     1321 gagctggtgg ccaaagcgat tcaagccgta gcctacgaat ggacacgccg tggcccggcc
     1381 acacaattga ccgccctgtt gttgaaacac acgggtacac acaccgtgga aagttttttg
     1441 gaccgcatga tgaatggccg gctcaacctt gacgctgcgg cagccccgct ggtttttcaa
     1501 gcggcccaca acggcgatga agtggccttg aatttgattc gctgggccgg acatgaattg
     1561 gccgagttag cgaaagccgt tattcgccaa ctcgatcttg cgccgctggc cttcgatgta
     1621 gtca
//
LOCUS       DASRTJ010000021         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_1096,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000021 DASRTJ010000000
VERSION     DASRTJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2614)
                     /locus_tag="VFF70_00385"
     CDS             complement(<1..2614)
                     /locus_tag="VFF70_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZY43179.1"
                     /translation="MAVVDDFEARREKLTPAKRALLDKRLRGEAWVVSAPIQPRSNQA
                     WRPVSFTQERFWLVNQLDGADPRYNMSSIYRITGELDVARLQYALQAIVDRQAVLRTN
                     FVAVQGVPQPIINDHAQINFQVFELTTLPPQEAETRSLQLIQQEIQRPFDLTRDVMLR
                     AVLIKQSDGQFLLALIRHHIATDGWSTRIIQQELTALYLQANSLPVLATQYADYAAWQ
                     RAQFDQGAFEKDIAFWKQQLAGSLPILELPTDHPRPSLQTWRGASEYLMLPVDLVKRA
                     REFSRRERLTLFMTLLASFDVLLYRYSAQADILVGTPVANRLHAETEKMIGPFFNMLV
                     MRSRLSGDLSFRNLMSQVRDTTLTAYDHQRAPFEKIVEVIQPDRDLGHSPVFQVMFAL
                     QNIPPSPLNLVGLAVEEVEIDRGVAQYDLTLEISEQTDGLRCSIEYNTDLFEAGTISR
                     MLGHYQNILAAAIVDPDRSIATLPLLTEAERRQLLIEWNLTAQAYPLDQCLQHLFQEQ
                     VKRTPDRLAVIFKDAHLTYAELNKRANRLAHYLIGCGVKPDDLVAIYFEPSLEMVVAI
                     LGTLKAGGAYVPLDPSNPARRRDLMLRDSGAKIILTQHRLAAQLLPNAGIILSLDADR
                     SLFEDESGLDPMTVCRPENLAYVIYTSGSSGQPKGVMIEHRSVVNNAQYCIQMLGLRP
                     NDRVLQFASISFDGSIEEIFPTLLSGAGLVLRPKELELNLKDFLDFVRVQHLTVLEPP
                     TAFWHQLTQDMVEGSTQLPAAIRLLFVGGEKASAAVLAAWLKIEGERVRWLNGYGPTE
                     ATSSVITYEAFASLPSINPLTEVPIGRPDPNTQAYILDDLLQPVPVGVPGELHIGGVC
                     LARGYLHQPALTTQK"
     gene            complement(2614..>2904)
                     /locus_tag="VFF70_00390"
     CDS             complement(2614..>2904)
                     /locus_tag="VFF70_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZY43180.1"
                     /translation="VEEALALIWQEVLHVDRTGVQAEFLQLGGDSILATQLIARIQQK
                     YQLSLSLVEFFAAPTISDQARILERLLLDEIEALSDDDLSIKKVELTRSGQD"
BASE COUNT          687 a          782 c          768 g          667 t
ORIGIN      
        1 atttttgcgt ggtcagcgcc ggttgatgca aataaccgcg cgccaagcat accccgccaa
       61 tgtgcaactc gcccggtacg cccaccggca ccggctgcaa aagatcgtca aggatgtacg
      121 cctgcgtatt tggatctggc cggccaatcg gtacttcggt gagcggattg atcgacggga
      181 gtgatgcaaa tgcttcataa gtaatgacgc tggatgtcgc ctcggtcggg ccatagccat
      241 tcaaccagcg tacacgctcg ccttcgattt ttagccaggc cgctaacaca gccgccgagg
      301 ctttctcgcc tccgacaaat aggagtcgaa ttgccgccgg cagctgtgta ctaccctcaa
      361 ccatatcctg cgtcaactga tgccaaaatg cggttggcgg ttctagcacc gtcaggtgct
      421 gtacccgcac aaaatcaaga aaatccttca ggttcaattc gagttcttta gggcgcagga
      481 ccaaacccgc gccacttaat aaagttggaa atatttcctc aatcgagcca tcaaaactaa
      541 tggacgcgaa ttggagcaca cggtcgtttg gccgaagccc gagcatctgt atacaatact
      601 gcgcgttatt gaccaccgat cgatgctcaa tcatcacgcc cttgggttgg ccagatgagc
      661 cggaggtgta aatcacatac gccagatttt ccggccggca gaccgtcatc ggatcgaggc
      721 cgctctcatc ttcaaacagc gatcgatcgg catccaagga gagaatgatc cccgcattag
      781 gcagcagctg agcggccagt cgatgttgag tgaggatgat ttttgcaccg ctgtcgcgca
      841 gcatcaaatc ccttcgccgg gcgggattgg acggatcgag cggcacgtaa gcgccgccag
      901 cttttaaggt gccgagtatc gccacaacca tctctagcga aggctcgaaa tagatcgcca
      961 ctaaatcgtc cggtttcacc ccacagccaa tcaggtaatg tgccaatcga ttcgcgcgct
     1021 tattcaattc ggcgtaggtc aagtgtgcat ctttgaaaat caccgctagt cgatcgggtg
     1081 tgcgcttgac ctgctcttga aataaatgct ggagacattg atccagcggg taagcttgcg
     1141 ccgtcagatt ccattcgatt aagagttgcc gacgctcagc ttcagtgagc aagggcagcg
     1201 tggcgatcga tcgatccgga tcgacgatgg cggccgccaa gatattttga taatgcccca
     1261 acatccggga gatagtcccg gcttcaaaca gatcggtgtt gtactcgatc gagcaacgca
     1321 acccatcggt ttgctcgcta atttccagcg tcagatcata ttgtgccacg ccgcgatcga
     1381 tttcgacttc ctcaacggcg aggccgacca aattcaatgg cgatggcgga atattttgta
     1441 aggcaaacat cacctggaac acgggcgagt gacccaagtc ccgatcgggt tgaatcactt
     1501 cgacaatttt ctcgaagggc gcgcgctgat gatcataagc ggtcaaggta gtgtcgcgca
     1561 cttgcgacat caagttgcgg aaactgagat cgcccgataa gcggctgcgc atgaccagca
     1621 tattgaagaa tggtccaatc atcttttcag tttcggcgtg cagtcgattg gcaaccggcg
     1681 tgcccactag aatatcggct tgcgcactgt agcgatataa cagcacatcg aaactcgcca
     1741 gaagtgtcat gaataaggtt agtcgctcgc ggcggctgaa ttcccgcgcg cgcttaacta
     1801 aatccacggg caacatcaaa tattcggatg ctccgcgcca ggtctgcagt gaaggccgcg
     1861 gatgatcggt ggggagctct agaatcggca gcgacccagc cagctgctgc ttccaaaaag
     1921 cgatgtcttt ttcaaatgca ccttgatcga attgcgcgcg ttgccacgcc gcgtaatctg
     1981 catattgagt tgcgagcacc ggcagcgagt tggcctgcaa atataatgcc gtcaattctt
     2041 gctggatgat cctagtcgac cagccatccg tcgcaatatg gtgccggatc agcgcgagga
     2101 gaaattgccc gtcgctttgt ttaatcaaga cggcgcgcaa catcacatcc cgcgttagat
     2161 cgaatgggcg ttgaatttct tgctggatga gctggagcga tcgagtttca gcttcttggg
     2221 gtggcaacgt ggtaagttca aatacctgaa agttgatttg tgcatggtcg ttgatgatcg
     2281 gctgcgggac tccctgcacg gcaacaaagt tcgttcgcaa gacggcctgt cgatccacaa
     2341 tcgcttggag agcgtattga agccttgcaa catcgagttc gcccgtgatg cggtagatgc
     2401 tcgacatgtt gtaacgcggg tcggctccat cgagctgatt gaccagccaa aatcgctctt
     2461 gagtaaacga caccggcctc caggcttgat tggatcgggg ctggattggc gctgatacta
     2521 cccacgcttc gccgcgcaag cgcttgtcca gcagcgcgcg tttggcagga gtcagttttt
     2581 ctcgacgtgc ctcaaaatca tcaacgacag ccattaatct tgtccggatc gggttagttc
     2641 tacttttttg attgaaagat catcatcgct taaggcttcg atttcgtcca acagcagccg
     2701 ctccaagatt ctggcttgat ccgaaatagt tggcgcggca aagaattcca ctagcgagag
     2761 ggacagctga tatttttgct ggatgcgcgc aatcaattgt gtagccaaaa ttgaatcgcc
     2821 gccgagttgc agaaattcag cctgcacgcc agtccgatcg acatgcagca cctcttgcca
     2881 aatcagagct aacgcttctt ctac
//
LOCUS       DASRTJ010000022         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_228269,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000022 DASRTJ010000000
VERSION     DASRTJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228269"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFF70_00395"
     CDS             <1..623
                     /locus_tag="VFF70_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HZY43181.1"
                     /translation="SVSLEQWKVEYSQSIRQDESQKSQAVTLGNVTEHFVPYLRDFIF
                     NPKDAHFIGSPVDFIVFDGLNDGEVKSVVFIEVKTGTSGLSSTERGIKEAVQSGKVQW
                     IELNPQLESQQPVEVDSSPRSAAPVEEETEFDVVLIGSGEKKIEVFKVMREVLPKLSI
                     NEAAHIVDGAPQTVLSAVSKEAAAAAKTKLEATGASVEVKHHVSVG"
     gene            complement(605..730)
                     /locus_tag="VFF70_00400"
     CDS             complement(605..730)
                     /locus_tag="VFF70_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43182.1"
                     /translation="MKMKRGGLIDQAASLFYWVIASLIYSTAQTRSEGKIYPTDT"
     gene            complement(741..1076)
                     /locus_tag="VFF70_00405"
     CDS             complement(741..1076)
                     /locus_tag="VFF70_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZY43183.1"
                     /translation="MKSEKGVYEITVEELSSRMKSGDVLTIIDVREPFEYNFAHIEGA
                     VLKPLPDLMEWAGELDKDQEYVLQCHTGSRSAYATTVLTQMGFKQVKNLVGGIAEWSA
                     RIDPNVPQY"
     gene            complement(1073..1828)
                     /locus_tag="VFF70_00410"
     CDS             complement(1073..1828)
                     /locus_tag="VFF70_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HZY43184.1"
                     /translation="MSNPAVTFQPTRRSDRFMFWLSAHWLLAFNVVWGAFVIAPWLAP
                     VLMRAGAPGVANGIYWFYQFFCHQLPERSYFLFGPQPMYSLQQVQIVFPATDPNVLRQ
                     FVGNADFGFKVAYSDRMVAMYTAFWAASIGFSAVRRRVQPLPIWGYILMLLPMAIDGG
                     SHFLSDLQAGANFGHGFRDANMWLAQLTNNAFPAAFYAGDALGSFNSIMRILTGVLFG
                     IASVWLAWPYAEAAFAEAREELEYRWKKVKSEK"
     gene            complement(2007..2276)
                     /locus_tag="VFF70_00415"
     CDS             complement(2007..2276)
                     /locus_tag="VFF70_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43185.1"
                     /translation="MKVTIDRNICGASLNACEHCFSFFAQHPMGVDRYCIVEQAEDGS
                     DLLTLTLRTEGHENTLVLDDAQREMVAAEGWSSLVDFVPKFYRAG"
     gene            complement(2539..>3808)
                     /locus_tag="VFF70_00420"
     CDS             complement(2539..>3808)
                     /locus_tag="VFF70_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43186.1"
                     /translation="MAPIKRVAMLSVHTCPLATLGGKETGGMNVYVRDLARELSRRGI
                     AVNVFTRSQNSHLPHVMHKLGSLGRVIHIPAGPEEPYDKNQIYDHLPEFVEGVKRFAA
                     DENIQYDVIHSHYWLSGWVARELRDVWHAPIVQMFHTLGKMKNSIAQSQAEMESERRI
                     EMESEIVQFADRLVAATPAEREQLIKLYGADPETISIVPPGVDLNLFQPMPTEIARQH
                     INVDVGPHDWMVMFAGRIEPLKGVDTLIRAMALLAHECPSWVDRLCLAIIGGDPDTNE
                     NAEMERLKQMHHELQLGDLVVFLGAKDQDTLHYYYNSADVLVMPSHYESFGMVALEAM
                     ACGTPVVASDVGGLSYLVRDGETGFHVPDGDFVALASTLARLLQDKQLRRNLGDEAMR
                     WAQTYAWAHIADRILGVYREARSMPLSIAA"
BASE COUNT          970 a         1049 c          987 g          802 t
ORIGIN      
        1 cgtcggtaag tcttgagcaa tggaaggttg aatactcaca atcaatcaga caagacgaga
       61 gccagaaaag ccaagccgtg acgcttggaa atgttaccga gcactttgtt ccgtatctgc
      121 gagatttcat ctttaatcca aaagatgccc attttatagg aagtccggtt gactttattg
      181 tcttcgatgg gttaaacgac ggcgaggtaa agagtgtcgt ctttattgag gtgaaaaccg
      241 gcacatcggg cttaagtagc acggaaagag gaataaaaga agcagttcaa tctggcaagg
      301 ttcagtggat agaactaaat ccacaattag aatcgcaaca gcctgttgaa gtcgattctt
      361 ctccgaggag cgcagctccg gtcgaagagg agaccgaatt cgatgtggta ttgataggca
      421 gtggcgagaa gaagatcgaa gtgttcaagg taatgcgcga ggtactgccc aaactcagta
      481 taaacgaagc agcgcacatt gtcgatggcg ctccacagac tgtgctgagc gcagttagca
      541 aagaagcggc tgccgcggcc aagaccaagt tggaagcaac gggcgcgagc gtcgaagtca
      601 agcatcatgt gtccgtaggg tagattttgc cctccgaccg cgtctgcgca gtagaataga
      661 tcaatgaagc aatcacccaa taaaacagcg aggcggcttg atcgatcagg ccgcctcgtt
      721 tcattttcac atttcactca tcaatactgc ggcacgttcg gatcgatccg cgcggaccat
      781 tcggcgatgc cgccgacgag gttcttcacc tgcttgaagc ccatttgcgt taagaccgtc
      841 gtggcatacg cgctgcgcga tccggtgtgg cactgcagca catactcttg atctttatcc
      901 agttcacccg cccactccat cagatcgggc agcggcttca acaccgcgcc ttcgatgtga
      961 gcaaagttgt attcaaacgg ctcgcgcacg tcgatgatcg tcagcacgtc gccggatttc
     1021 atccggctgg aaagttcttc gacggtgatt tcatacacgc ctttttcact cttcactttt
     1081 cactcttcac ttttttccat cgatattcca attcttcgcg cgcttcggca aaggccgcct
     1141 cggcatacgg ccaggccagc caaaccgacg caatcccaaa caacacaccg gtcaagatac
     1201 gcatgatcga attgaaggag cctaacgcat cgcctgcgta gaatgccgcc ggaaaagcat
     1261 tgttggttaa ttgcgccagc cacatatttg catcacgaaa gccatgaccg aaatttgcgc
     1321 ccgcttgtag atcgctgagg aagtgtgacc cgccatcgat cgccatcggc agcagcatca
     1381 agatgtaacc ccagatcggc agcggttgca cgcgccgtcg gaccgcagaa aaaccgatcg
     1441 aggcagccca aaaagccgta tacatcgcca ccatccgatc ggagtaagcg actttgaaac
     1501 caaagtcggc attgccgaca aattggcgca ggacattcgg atcggtggcc gggaagacga
     1561 tttggacttg ctgcagagaa tacatcggct gcgggccgaa gaggaaatac gatcgctcgg
     1621 ggagttgatg gcaaaagaat tggtagaacc aataaatgcc gttggcaacg ccgggggcac
     1681 cggccctcat caacacgggt gccagccaag gtgcgattac gaatgcgccc cacaccacgt
     1741 tgaacgccaa caaccagtgc gctgacaacc agaacatgaa tcgatctgag cgtctggttg
     1801 gttgaaaggt gacggctgga ttcgacatga tctgctcccg ataaactcaa cacatcgatc
     1861 ggtgatgctg gcggatcgac gccatgattg taccctgaag ccagatcggc gtaaagcaaa
     1921 cttcgatcag aaccggatca aaaaagaggg cggcagctgc gtgataacgc acagctgccg
     1981 ccagagaaaa aaggtgggtt gttgaattat ccggcgcgat agaacttcgg gacaaaatca
     2041 accagtgaag accagccttc ggcggcgacc atttcgcgtt gagcatcatc cagcaccaaa
     2101 gtgttctcat ggccttcggt gcgcagcgtc agggtcaaca aatccgagcc atcttcggcc
     2161 tgttcgacga tgcagtatcg atccacgccc atcggatgct gggcgaagaa cgagaagcaa
     2221 tgctcacagg cattcaaact tgcgccgcaa atgttgcgat cgatcgtgac tttcatatta
     2281 gagcctcctc acctatttac agttaacaag atgagcatac tcttttttgg cggcaactta
     2341 aagtgctaaa gtaatcatga tcgatgtgat aagtgtcatg catgcgaggt gactgcaaac
     2401 aacgtggctc gatcggataa ataaccagat aaagttcgtc tttaatccga tcgatcttct
     2461 ggtaaataaa aaacggcggg cgacctgccc tagtaacgaa gtggataagg ttgtccgcca
     2521 cgagcaacct gcgtaacctt acgctgcaat actcagcggc atcgatcggg cttcgcgata
     2581 cacacccaaa atccgatcgg cgatgtgcgc ccaagcgtaa gtctgcgccc aacgcatggc
     2641 ctcgtcgcct aaattccgcc gcagctgctt atcctgcagc aagcgggcca gggttgaagc
     2701 caacgccacg aagtcgccgt ccggtacgtg aaaaccggtt tcgccatcac gcaccagata
     2761 gctcaagcca cctacatccg aggcaaccac cggtgtaccg cacgccatcg cctctaacgc
     2821 gaccatgccg aacgattcat agtgcgacgg cataaccaac acatccgctg agttgtaata
     2881 ataatgcaag gtgtcctgat ctttcgcgcc gaggaacacc accagatcgc ctagctgcaa
     2941 ttcatgatgc atttgcttca agcgttccat ctcagcgttc tcatttgtat ccggatcgcc
     3001 gccgatgatc gccaggcaca atcgatcaac ccaactggga cattcgtgtg ccaacagcgc
     3061 catcgcgcgg atcaacgtat cgacgccttt caatggttcg atccggcccg cgaacatcac
     3121 catccagtcg tgcggaccaa catcgacgtt gatatgctga cgcgcgatct cggtgggcat
     3181 gggctggaac aaattcagat cgacgcccgg cggcacgatc gagatcgttt ccggatcagc
     3241 gccatataac ttaatcaact gctcgcgctc ggccggggtg gcggccacca gccgatcggc
     3301 aaactgcacg atctcggatt ccatttcgat gcgtcgctcg gattccattt cggcttgact
     3361 ttgagcgatc gaatttttca tcttgcccag ggtgtggaac atctgcacga tcggcgcgtg
     3421 ccatacatcg cgcaattcgc gcgcgaccca gccggacaac caatagtgac tgtgaatcac
     3481 atcgtactga atattttcgt ccgcggcaaa acgtttgacg ccctcgacaa actcaggcaa
     3541 gtgatcatag atttgatttt tgtcgtacgg ttcttccggt ccggcgggaa tgtgaatgac
     3601 gcggcccagc gagccgagtt tgtgcatgac gtgcggcagg tgcgaattct gcgatcgagt
     3661 gaacacattg acggcgatgc cgcgccgcga caactcgcgc gccagatcgc gcacgtagac
     3721 attcatgccg cccgtctctt tgccgccgag cgtcgccagc ggacaagtat gcacgctcaa
     3781 catcgctaca cgtttaatag gagccatg
//
LOCUS       DASRTJ010000023         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_304115,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000023 DASRTJ010000000
VERSION     DASRTJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304115"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFF70_00425"
     CDS             <1..528
                     /locus_tag="VFF70_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43187.1"
                     /translation="LTGGLDAEKYLAHSAHPWAPACNVIYRRSIFKAIGGFDERYLNY
                     EYCDLHLRMRHHSDLVFKHEPRALVLHWHRSTWRQYWHQQFFYGRGYGQFLWNYRDQF
                     HWEPWREVKAWGQLVGLAIRAAWPGQDDRSIVRCGLFIKQFAQHLGFISTYYNRAERA
                     RWLNQSTASLVTSGG"
     gene            complement(492..995)
                     /locus_tag="VFF70_00430"
     CDS             complement(492..995)
                     /locus_tag="VFF70_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis protein"
                     /protein_id="HZY43188.1"
                     /translation="MPKFAYKWTPAGIWQRVHQMIRQPSDVILFWRLGYFIWSAPGRL
                     NRSSLPTLLKNYRSSPRPKAIDPNAAIDRINRLSQPWLRRSFFRARNTCYLRSLMMFR
                     YLDPAGHDLRINFVVEPAINPGDRLKGHAWVTIDGAILESTAPDIIERTQQLYIYPPE
                     VTSDAVD"
     gene            complement(988..1914)
                     /locus_tag="VFF70_00435"
     CDS             complement(988..1914)
                     /locus_tag="VFF70_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43189.1"
                     /translation="MASNISVRVVIPVYNGADTIGDLLTALTHQVDPPLDCEIIVVDN
                     GSTDRTCEVVRSFGAALLEESERGPGAARNRGLVAARGEVIAHLDADVVPTRQWLKKI
                     VEPFNDPETIMVGGATYNYKPETAAQRYMASLPDARLERRIGQSTFPFIASRNMAVRC
                     ESALAIGGWATDMPTAEDADFCLRLWRKFRAPICHQPTAIAFHHDRATLAELFHQAFT
                     YGEGLARMYFKYPELGPWSFWNTIQLSWVLMKQALVSRALSLGHSIGLDPANRAELVA
                     IHRAWMQSYWEGFRSMRQDLQRRNALRGNVNA"
     gene            complement(1904..2854)
                     /locus_tag="VFF70_00440"
     CDS             complement(1904..2854)
                     /locus_tag="VFF70_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43190.1"
                     /translation="MRRLYPQEMQKIAEAQRQPVSTSELTRSDQPSVTVVILSLDRLH
                     LTRRCIDSVYANSDYPFQLLIWDNGSQPETIAYLKQLEAVHANVQVIYSGTNVGCGGG
                     RNRAFRLVDTDYIFSMDNDMICHADWLRETMACVVRQAASFVAPMRLNPAGQVWSACN
                     ELIHRQETDTLEIARWFHDLPIAVVQSLFKDADLPTNFMSGGLGLFATRVFHEIGEFD
                     ENYRAGFEDLDISLRLSDQGYRVWSTVRALVTHDDEWQPHLQADFVYAQQRYDLDQLR
                     CDADHFKARWGLDVLPEKYVTRFQQRLDLKLKAESDRYGL"
     gene            complement(2860..3975)
                     /locus_tag="VFF70_00445"
     CDS             complement(2860..3975)
                     /locus_tag="VFF70_00445"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZY43191.1"
                     /translation="MRPEDKILLAATRQNFPLEYQQAVLAACQRSHIDWPALFLTAVN
                     HGVEPLVYYNLKQISPAKLGVPPAVIDKFKQHMTHNVFVKDHMAELLPDILSLFERYQ
                     VEVMLVKGAALDRLIYARPWSTTSEDIDLLLKPTRSELEQIDGGAIITALKVINKQAD
                     RLKLHIEYDCYAHHDLNMNGVLPIDFDRIWRDASAFNIGEHRVAIMAPEDLLIGACVN
                     CCRKRFFRLKALCDIAEIISRLPALNWPLFIEKANSYQCDNIVYAALIVTRATIGCDV
                     PSIVLSELKTSRVRKFFIRMTARSLKRVCSLDTLSRYSGRKIFRRVFGLSLVLPYVTY
                     RWDQVGRKAQELDRAWKQLAIGDEDGPNGSDRSQEIK"
BASE COUNT          952 a         1141 c         1032 g          872 t
ORIGIN      
        1 ctcaccggcg gactcgacgc ggaaaaatat ctggcccact cggcgcatcc ctgggcaccg
       61 gcctgtaacg tcatctatcg gcgtagtatc tttaaagcga tcggtggctt tgacgagcgt
      121 tacctcaact atgaatactg cgatctacat ctgcgcatgc gccaccacag cgacttggtc
      181 ttcaaacatg aaccccgcgc gctggtgttg cactggcatc gatcgacatg gcgccaatat
      241 tggcatcaac aattttttta cgggcgtggc tatggtcaat tcctctggaa ttatcgcgat
      301 caattccact gggagccgtg gcgcgaagtg aaagcctggg gacagcttgt cgggttggcg
      361 atccgtgccg catggcctgg ccaagacgat cgatcgattg tgcggtgtgg cttgtttatc
      421 aagcagttcg cacagcatct gggtttcatc agcacttact acaatcgggc cgaacgcgcg
      481 cgctggctga atcaatctac tgcatcgctc gtcacttccg gcggataaat atacagctgc
      541 tgcgtgcgct cgataatatc cggcgcggtc gattcaagga tcgcgccatc gatcgtcacc
      601 caggcgtgac ctttcaggcg atcgccgggg ttgatcgccg gttcgaccac aaagttgata
      661 cgcagatcat gtcctgccgg atcgaggtaa cgaaacatca tcaatgatcg caaatagcag
      721 gtattgcggg cgcggaagaa ggatcggcgc aaccacggtt ggcttagacg attgatccga
      781 tcgatcgcgg cgttgggatc gatcgcttta ggacgcggtg acgatcgata atttttcagg
      841 agcgtcggca aactcgatcg attcaaccgg cccggcgcgc tccagatgaa atagcccagc
      901 cgccagaaca aaatcacatc ggacggctgc cggatcattt gatgcacgcg ctgccagatg
      961 ccggcgggtg tccacttgta agcaaattta ggcattcaca ttccctctaa gcgcgttacg
     1021 ccgctgcaga tcttggcgca tactcctaaa gccttcccag taactctgca tccaggcgcg
     1081 atgaatcgct accagctcgg cgcgattagc cgggtccagg ccaatcgagt gaccaagact
     1141 taacgcgcgc gagacaagcg cttgcttcat caacacccaa ctcaattgga tagtattcca
     1201 gaaactccac ggcccaagtt ctggatattt gaaatacatg cgcgccaagc cttcgccata
     1261 ggtaaacgct tgatgaaaaa gttcagctaa cgtagcacga tcatgatgaa aagctatggc
     1321 cgtcggttga tggcaaatgg gcgcgcgaaa ttttctccaa agtcgtaaac aaaagtcggc
     1381 gtcttctgcg gtcggcatat cagtggccca accgccgatc gccaacgcag actcacaccg
     1441 gacagccata ttgcgggaag cgataaacgg aaaagtggac tgcccgatcc tgcgttccag
     1501 acgcgcgtcc ggaagactcg ccatataacg ctgcgccgct gtttcgggtt tatagttata
     1561 agtcgcgccg ccgaccatga ttgtctcagg atcattgaaa ggttcaacaa tttttttgag
     1621 ccactggcgc gtaggcacaa catccgcatc cagatgcgcg atgacctcac cacgcgctgc
     1681 aaccaagccg cggttgcgcg ccgcgccggg tccgcgctcg ctttcttcca gcagcgcggc
     1741 cccgaacgag cggacaacct cgcacgtccg atcagtcgag ccgttatcga cgacgatgat
     1801 ctcacaatcg agcggcggat cgacttgatg cgtcagcgcg gtcagcagat cgccgatcgt
     1861 gtccgcgccg ttatagaccg gtatcaccac cctcactgaa atgttagagg ccatatcgat
     1921 ctgattcagc tttcagttta agatcgagcc gctgctgaaa gcgggtgaca tatttttccg
     1981 gcagaacgtc cagaccccag cgcgctttga aatgatccgc gtcacagcga agttgatcga
     2041 gatcgtaacg ttgttgcgcg tacacgaagt ccgcttgaag gtgtggttgc cattcgtcgt
     2101 cgtgcgtcac taaagcccgc acggtcgacc aaaccctata accttgatca ctcaagcgca
     2161 gtgaaatgtc taaatcttca aaaccggcac gatagttttc gtcgaactcg ccgatctcat
     2221 gaaagacgcg agttgcgaat agtccaaggc cgccactcat aaaattggta ggcagatcag
     2281 cgtccttgaa aagtgactga accacggcga tgggcagatc atgaaaccaa cgagcaatct
     2341 caagtgtatc cgtctcctgc cggtgaatca actcattgca cgcggaccac acttgcccgg
     2401 caggattgag ccgcatcggc gcgacgaagg acgccgcctg ccgcacaaca caggccatgg
     2461 tctcacgcaa ccaatctgcg tggcagatca tatcgttgtc cattgagaaa atataatcgg
     2521 tatcaaccag ccggaaggcg cgattgcgcc caccaccgca gccgacattc gtaccagagt
     2581 agatcacctg cacgttagcg tgcacggcct caagttgttt caggtaagca atcgtctctg
     2641 gctgggagcc gttgtcccaa atcagcaact gaaatggata atcgctgttc gcatatacgc
     2701 tgtcaataca gcggcgcgtg aggtgcaatc gatcgagcga caggatcacc actgtgacgc
     2761 tcggctgatc cgatcgagtc agctcgctcg tcgaaaccgg ctgacgttgc gcctcggcga
     2821 tcttctgcat ttcctgcgga tatagacgtc tcatgcgggt catttgatct cctgcgatcg
     2881 atcactcccg tttggaccat cttcatcccc aattgctaat tgcttccacg cccgatcgag
     2941 ctcctgcgct ttgcggccaa cttgatccca gcgataggtc acatacggca gcaccaacga
     3001 taacccaaac acacgcctga aaatttttcg gccgctgtaa cgtgacagtg tatctaacga
     3061 gcagactctc ttcaagctgc gtgccgtcat tcggatgaaa aactttctca cccgcgaagt
     3121 cttcaactca ctcaatacga tcgacggcac gtcacagccg atcgtcgccc gggtaacaat
     3181 caacgcggcg tagacaatgt tatcgcattg ataactgttc gctttttcaa tgaagagcgg
     3241 ccagttcaaa gccggcaacc ggctgataat ctcggcgatg tcgcataacg cctttaggcg
     3301 aaagaaacgc ttgcggcagc agttgacaca cgcgccgatc aacaaatcct ccggcgccat
     3361 gatcgccact cgatgttcac cgatgttgaa cgctgaggca tcgcgccaga tgcgatcaaa
     3421 atcgatcggc aggacgccat tcatattgag atcatggtgg gcgtagcaat cgtactcgat
     3481 gtgcagcttc agccgatcgg cttgcttgtt gatcaccttt agagctgtaa tgatcgcgcc
     3541 gccgtcgatc tgttccaatt cactgcgcgt cggcttgagt aagagatcga tatcttcaga
     3601 cgtggtcgac cagggtcgcg cgtagatcaa tcggtccagc gccgcaccct tgaccagcat
     3661 cacctcaacc tgataacgct cgaacaggga cagaatatcc ggcagcaatt ccgccatgtg
     3721 atctttgaca aagacgttgt gcgtcatgtg ctgtttaaat ttgtcgatca cagcgggtgg
     3781 cacaccaagt ttcgccggag aaatctgctt gaggttgtaa tacaccaacg gctcaacgcc
     3841 gtgattaacg gccgttaaaa aaagcgccgg ccagtcgatg tgactgcgct ggcacgccgc
     3901 gaggacggct tgctggtatt ccagtggaaa attttgccgc gtagccgcca acaaaatttt
     3961 gtcttcgggt cgcataacgc actcattata tcagcac
//
LOCUS       DASRTJ010000024         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_304121,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000024 DASRTJ010000000
VERSION     DASRTJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304121"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFF70_00450"
     CDS             <1..333
                     /locus_tag="VFF70_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZY43192.1"
                     /translation="MLMNVWERRREIGALRSIGMTRAQIIRMILAEAGAMGAIGAVFG
                     IGFGVLLSRVFIEGMRVSIGYRIEFTLPFSSILLGLAIAFLVSQAAALYPAWRGSRVN
                     IVEAIKHE"
     gene            406..1593
                     /locus_tag="VFF70_00455"
     CDS             406..1593
                     /locus_tag="VFF70_00455"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZY43193.1"
                     /translation="MSRNRRIFLVIGIIVLSAIGFAGYQAFLAPTPPTPAPASVIKAS
                     EDVVSAQAFVAPRQHSDLAFRSGGRVAEVLVSEGEQVMPGQALVRLQDDQLKAALAQT
                     HAALNAAQANEDLVAEGARPEEIAQAEAAVRAAEAQVTAAITDRDRLTGGGTDASIAA
                     AQSQLAAALVEQKLAQDALDKLPDFVHGTPEEQLRYRVNAANQAVNAAQKNLDEALSG
                     SGQQVKAAQSNIAAAIAQRDIAQAQLEQLRNGATAAQLAAAKANTAQAQAAINAALAA
                     LDEATLKAPFAGSVAFIDVEVGQVIAAGLTVASIADLRSWDVDTDDLSEVDVVNVQAG
                     QAATITLDALPGILLSGTVLSVTPKSETKRGDVTYTAKIKIDNPDPRLRWGMTAQVNI
                     TVK"
     gene            complement(1689..2075)
                     /locus_tag="VFF70_00460"
     CDS             complement(1689..2075)
                     /locus_tag="VFF70_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43194.1"
                     /translation="MSQAADAQPCPRCGSRVHRVHRQPLDRLISIVLPRQRYRCAQCG
                     WTGVRRASGRSNEGHAVGLRRKQVVLIVVLLTLMLVAGVAMTRIVFDQSTPSNGRPRR
                     RSGQVMPQINMLYVAAGDLRLDWSDS"
     gene            complement(2099..2905)
                     /locus_tag="VFF70_00465"
     CDS             complement(2099..2905)
                     /locus_tag="VFF70_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HZY43195.1"
                     /translation="MNRRVLLGVATFALAIFIYGAVARILLAPIVQLPALPGGTSTLT
                     LLLMIFSLLHARYLLGWRHTLLFFALSTVISLIYEEVGVGTGLIFGPYHYSDVLGMKL
                     GLVPILIPIAWFAMIYPSYVIANLITDGQPSGSRGGLARIAWVSLLSAMVMTAWDLLI
                     DPILSHPNVHAWVWDRLGSYFGIPIHNYLGWMLTTFTVYLVYRIFERRSAPQPVGAIT
                     NWIAALAIIAYGSMMLGNMLTGPAALIVIGPFAMGLPIVFALDRLWHRSS"
     gene            3103..>3704
                     /locus_tag="VFF70_00470"
     CDS             3103..>3704
                     /locus_tag="VFF70_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZY43196.1"
                     /translation="MTQKTAFVFSGVLTAFLLVVGGGLVGRMAQTEATPAPTAAVAAP
                     VAVADPASQVIAQLQDREAAYQQLIDQANQRLQQAYDQQKATAAKLNQTRSVAAAPRT
                     QQPAVAAAAQPAAPALSAESAINIAIDASGGKRMTRTPELVMFEGSVAYEIGFTNGAI
                     YVDANSGSVLFNGVTAPHAGGGSTASVQPPASSGEPTDSH"
BASE COUNT          906 a         1051 c         1031 g          716 t
ORIGIN      
        1 atgttgatga atgtttggga acgccgccgc gagatcgggg cgctgcgatc gatcggcatg
       61 acgcgcgcgc agatcatccg catgatcctg gccgaggccg gggcaatggg ggcgatcggc
      121 gcagtgttcg gcatcggctt cggcgtgctg ttgtcgcgcg tgttcatcga aggcatgcga
      181 gtgtcgatcg gctatcggat cgaattcaca ctgcccttca gttcgatctt gttgggcttg
      241 gcaattgcat tcttggtgtc acaagcggcg gcgttgtatc cggcgtggcg cggctcgcgc
      301 gtgaacattg tggaagcgat taaacacgaa taatacaagg atgatggaag aaggctgaat
      361 ttttcatgcc tccaccttca gccctatccc ttcaatggag gactcatgag tcgaaacaga
      421 aggatctttt tggtcattgg gatcatcgtg ctcagtgcga tcggcttcgc gggttatcaa
      481 gcgtttctcg cgccgacgcc gcccacgcct gcaccggcat ccgttattaa agccagtgaa
      541 gatgtggtct cagcacaagc ctttgtcgca ccgcggcagc acagcgatct ggcgtttcga
      601 tcgggcgggc gcgtggctga ggtcttggta tccgaaggcg agcaggtgat gccaggccaa
      661 gcgttggttc gcttgcaaga tgatcagctc aaagccgcac tcgctcaaac acacgccgcg
      721 cttaatgccg cgcaagccaa tgaagattta gtggctgagg gcgcgcggcc ggaagagatc
      781 gcgcaagcgg aagcggctgt acgtgcagct gaagctcagg tgaccgccgc gatcaccgat
      841 cgcgatcgat taacgggcgg tgggactgac gcctcgatcg ctgcggcgca atcgcaactc
      901 gcagcggcgt tggtcgaaca gaagttggcg caagatgcgc tggataagtt gccggacttt
      961 gtacacggca cacccgagga acagctgcgt taccgagtga atgccgcgaa ccaagccgtg
     1021 aatgccgcac agaaaaattt ggacgaagct ttatccggct cgggtcagca ggtaaaggct
     1081 gcgcaatcga acatcgccgc cgcgatcgcg cagcgcgaca ttgcacaagc gcagctcgaa
     1141 caattaagga atggtgcgac ggcggctcaa ctcgcggcgg ccaaagcgaa tactgctcaa
     1201 gcccaagccg cgatcaacgc agccctggcc gcgctcgacg aagcgacatt gaaagcgccc
     1261 ttcgccggca gtgtggcctt tatcgacgtt gaagtcggac aagtaattgc cgccggcttg
     1321 accgtcgcca gcatcgccga tctcagaagt tgggatgtgg acacggatga tctcagcgaa
     1381 gtcgatgtag tgaatgtgca ggctggccaa gccgcaacga tcacactcga cgcactgccg
     1441 ggtattttgt tgagcggcac tgtcctatcg gttacgccga aatcagaaac caaacgcggg
     1501 gatgtgactt acacggcgaa gatcaaaatc gacaatccag atccgcgttt gcgctggggc
     1561 atgaccgcgc aggtaaatat caccgtaaaa tgattggcga caagcgtatt gagatcaatc
     1621 catgaaaagt cccgtcgagg tcaacctcaa cgggactttt tgatcgcact aaatcaaatg
     1681 tccatcgatc aagagtcaga ccagtccaat ctcaaatcac cggctgcaac gtacaacata
     1741 tttatctgtg gcatgacctg ccctgatctt cttctgggcc gtccgttgct tggcgttgat
     1801 tgatcaaaga cgatacgcgt catagcgacg ccggctacca gcatcaacgt gagcaagacg
     1861 acaatgagca cgacctgctt gcgccgcagg ccaaccgcat gaccttcatt cgatcggccc
     1921 gacgcgcgac gcacgcccgt ccaaccgcac tgtgcgcagc gatagcgctg gcgcggcagg
     1981 acgatcgaga tcaatcgatc caacggctgg cgatgaactc gatggactcg tgagccacag
     2041 cgcggacagg gttgagcatc agcagcttga gacattattg actccattcg attaagcatt
     2101 agctcgatcg atgccacagg cgatcgagcg caaagacaat cggcagaccc atcgcaaatg
     2161 ggccgatgac gatcaacgcg gcagggccgg tcagcatgtt gcccaacatc atcgagccgt
     2221 aagcgatgat cgccaaagcc gcaatccaat tggtgatcgc accgacaggc tgcggcgcgc
     2281 tgcgccgctc gaatatgcga tagaccagat aaaccgtgaa cgtggtcagc atccaaccca
     2341 aataattatg gatcggaatg ccaaaatagg atcctagtcg atcccacacc caagcgtgta
     2401 cattgggatg cgacaaaatc ggatcaatga gtagatccca cgcggtcatg accatggcgc
     2461 tgagcagcga tacccacgca atgcgcgcca ggccgccgcg actaccgctt ggttgtccgt
     2521 ctgtgatcag attagcgatc acgtaactgg gatagatcat cgcgaaccac gcgatcggga
     2581 tcaaaatcgg cactagcccg agcttcatac ccagcacatc ggagtaatga tacggcccga
     2641 aaatcaaacc cgtgcctacc ccgacttcct catagatcaa cgagatcact gtgcttaacg
     2701 caaagaacaa caacgtgtgc cgccagccca gcaaataccg cgcatgcaac aaactaaata
     2761 tcatgagcag cagcgttaag gttgatgtac cgcctggcag ggccggcagc tgcacaatcg
     2821 gggccagcaa aatccgcgca accgcgccat aaataaaaat ggctaaagca aaggtcgcta
     2881 cacctaggag cacgcgtcga ttcatatcgg cctcatcagt agattcaaga cacgttggct
     2941 gcgaaaactt tcgctgccaa atgcctgtat tgtactagat gaaggccaga ttagaaaaac
     3001 aaataaccag acggcatcca ttaatctcac atgactcaag tacccgttac ttattgggcg
     3061 gtttcgcaac ttataaggta acttacccat tggaggttca gcatgactca aaaaactgct
     3121 ttcgtatttt ccggcgtatt aactgctttc ctgttggtgg tcggcggcgg attagttggc
     3181 cgcatggcac agactgaagc tacgcccgca ccgactgcgg ctgtggcggc acccgtcgcg
     3241 gtcgccgacc cggcgagtca ggtgatagcc cagctacaag atcgtgaagc cgcctaccag
     3301 caattgatcg atcaagcgaa tcagcgtttg caacaagctt acgatcaaca aaaagcaact
     3361 gcggccaagc tcaatcaaac ccgatcagtt gcagctgctc cgcgcactca acaaccggcg
     3421 gttgcggctg cagctcagcc ggctgcgcct gcgttgtccg ccgaatccgc aattaacatc
     3481 gccatcgatg cttccggcgg caagcgaatg acccgcacgc ccgagctcgt catgtttgaa
     3541 ggcagtgttg cttacgagat cggctttacg aatggcgcaa tctatgtgga cgccaattcg
     3601 ggctcagttt tgttcaacgg tgtcaccgcg ccgcacgccg gcggcggcag caccgcttcg
     3661 gtccagccac ccgcgagcag tggcgaacca acagactcgc atga
//
LOCUS       DASRTJ010000025         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_228434,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000025 DASRTJ010000000
VERSION     DASRTJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228434"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFF70_00475"
     CDS             complement(<1..944)
                     /locus_tag="VFF70_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43197.1"
                     /translation="MSGEEPLTPEEAEEEVEEIIEAAKRVGVEIDTEAAIEWMVAVSE
                     AEREGALAQDPFSGVFGHRISLLDFDQDHLDYFRRLGQRVRLARRSNVETAIAIAGSS
                     AQGKVQLFPGDNDFFERVNIKTPTLDAARKLLRDMVRETAIRAFLEPDIVLLEVNLGV
                     YPLAVIERGEPRGAGDSITWKPSDVANGYIVVARADDPNLSIRINWAEVQSGLGCTFF
                     GWIVADRESGRIVLASNMLDVTWEAPDGKLTSLDGSIDPFFQEIYLEAAALPVFTKIV
                     KQVEQSAMNAYINSMRWQVFHYTHQAPNYGKASKRLYNL"
     gene            complement(941..1495)
                     /locus_tag="VFF70_00480"
     CDS             complement(941..1495)
                     /locus_tag="VFF70_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZY43198.1"
                     /translation="MAHQTDTYISIDVEAAGPVPGEYSLLSIGACVVGAAEKNFYVEL
                     KPINFNFVQAAIDVAGFDLKALDRSGIDPREALRLFEEWLRSVAPIDPAPIFVAYPLA
                     FDWMFVAYYFQRFIGHNPFGYQGLDIKSYYAGLVGTRWSQSGHEQISAKLSIEFELTH
                     NAREDAIEQAKLFDRLLNYKKGEA"
     gene            1668..>2540
                     /locus_tag="VFF70_00485"
     CDS             1668..>2540
                     /locus_tag="VFF70_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZY43199.1"
                     /translation="MQGKLQRTGWLLLIAILLIFGSTRSIAAKIEPGDGQSSSSIYVL
                     HGIDRVEDRSAIAATGVDIIEVGKDYIVIAATPEQIDQIKQLGFAPSQFQALVASPSI
                     FVDPAYHTYAQMVANIQSYAITYTNIMQLITVGVSYEGRPIWVAKISDNPNIDEGESE
                     VLFTFHQHAREHLTVEQGLYTLHLLTDNYGSDITITQLVDTRVVWLFFDLNPDGGEYD
                     ISGGTYQGQRKNCQPVGCDSSNIGTDLNRNSDYGWGCCSGSSASPSDETYRGPSAFSE
                     PETTVISNFVATRVI"
BASE COUNT          600 a          727 c          632 g          581 t
ORIGIN      
        1 agattgtaca atcgcttgga agctttgccg taattcggtg cttgatgtgt gtaatgaaac
       61 acctgccaac gcatggagtt aatgtaggcg ttcatcgcgc tctgctccac ttgcttgaca
      121 atcttcgtga acaccggcag cgcggccgcc tctaaataaa tttcttgaaa gaacggatcg
      181 atcgagccgt cgagcgaagt caacttgcca tccggcgctt cccatgtcac atccaacatg
      241 ttgctcgcca acacgatgcg gccggattct cgatcggcca cgatccaacc aaaaaacgtg
      301 cagcccaggc ccgattgaac ttcagcccag ttgatcctga tcgataggtt cggatcgtca
      361 gcgcgcgcca cgacgatata accgttcgcc acatcagatg gtttccacgt tattgagtcg
      421 cctgccccgc gcggctcccc ccgctcaata accgccagtg gatatacgcc gagattcact
      481 tctaataaaa caatgtctgg ctctagaaaa gctcggatgg ccgtttcgcg caccatgtcg
      541 cgcagtaatt tgcgcgctgc atcgagcgtt ggcgttttga tattcacgcg ctcgaagaaa
      601 tcattgtcgc ccggaaataa ctgcacttta ccctgcgccg aactgcccgc aattgcgatc
      661 gccgtttcca cattcgatcg acgcgccaac cgcacgcgct gccccaagcg ccggaagtaa
      721 tccaaatgat cttgatcaaa atccagcagc gaaatgcgat gaccgaacac gccgctgaac
      781 gggtcttggg ccagtgcgcc ctcgcgttcg gcttccgaga ccgccaccat ccattcaatt
      841 gcggcctcag tatcgatctc cacgccgact cgtttcgccg cctcaatgat ttcttcgact
      901 tcttcttctg cctcttcggg cgtcaatggc tcttcgccgc tcatgcttct ccttttttgt
      961 aattcaacaa tcgatcgaac aacttagctt gctcgatcgc atcttcacgc gcattgtgtg
     1021 ttagctcaaa ctcgatggac aactttgcgc tgatctgctc gtgccccgat tggctccagc
     1081 gcgtcccgac gagacccgca taatatgatt tgatatccag accttgatag ccgaacggat
     1141 tgtggccgat gaagcgctga aaatagtaag ccacaaacat ccaatcgaac gcgagcgggt
     1201 aagccacaaa gattggcgcg ggatcgatcg gggcgaccga gcgcagccac tcttcaaaca
     1261 gccgcagcgc ttcacgcgga tcgattccgc ttcgatcgag agccttcaaa tcgaagccgg
     1321 ccacatcgat cgctgcctga acgaaattga aattgatcgg cttgagctca acgtagaaat
     1381 ttttttcggc cgcgcccacc acgcatgcgc cgatcgatag caaactatat tcgcccggca
     1441 ccggtccggc ggcttccaca tcgatagaga tgtaagtgtc agtttgatga gccatattat
     1501 tgttgtcatt tcgagcgaag agagaaatct cgttggtcaa gggcaagaga cctctcgctg
     1561 cgtccgaggt gacatgcgag ccagatacag actttgcgag acgtgccgct catgatatga
     1621 tgcggcttca gttcaaggga ttatacaacg caagggcgaa ggctaacatg cagggcaaac
     1681 tccaacgcac cggctggctg ctattgatcg cgatcctatt gatcttcgga tcgacgcgct
     1741 cgatcgccgc caagatcgag ccaggcgacg ggcaatcatc gtcatcgata tatgtcctcc
     1801 acgggatcga tcgggttgaa gatcgatcgg cgattgcggc aaccggcgtg gacatcatcg
     1861 aagtgggcaa agactacatc gtgatcgctg cgacaccgga acagatcgat caaataaaac
     1921 aactcggttt cgcgccgagt caattccaag cgttggtcgc cagtccatcg atcttcgtcg
     1981 atccggcgta tcatacgtac gcgcaaatgg tggccaacat ccaatcttac gcgattactt
     2041 ataccaacat catgcagttg atcaccgtcg gcgtatcgta tgaaggccgc ccgatctggg
     2101 tcgcgaaaat ttcggataat ccgaatatag atgaaggcga atccgaagtc ttgttcacct
     2161 ttcatcaaca tgcgcgcgaa catctcactg ttgaacaagg tttatatact ttgcatctgc
     2221 tcaccgacaa ctatggcagt gatataacga tcacgcagtt agtcgacacg cgcgtcgtct
     2281 ggctgttctt cgatctgaat cccgacggtg gtgagtacga tatcagcggc ggcacgtatc
     2341 aaggtcaacg caagaattgc caacccgtcg ggtgtgattc gagcaatatt ggcaccgatc
     2401 tcaatcgcaa ctcggattat ggctggggct gctgcagtgg atcgagtgcc agcccttcag
     2461 atgagaccta tcgcgggccg tcggcatttt ctgagccgga gacaaccgtg atcagcaact
     2521 tcgtcgctac tcgcgtgatc
//
LOCUS       DASRTJ010000026         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_228481,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000026 DASRTJ010000000
VERSION     DASRTJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228481"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFF70_00490"
     CDS             <1..946
                     /locus_tag="VFF70_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43200.1"
                     /translation="RDIAMVFQSYALYPHMSVYDNLAFSLRLRAKTGRVRKLVTLVFN
                     RPAYQKIKDQEANIDQRVHKAAQTLLIEQLLPRKPKQLSGGQRQRVAVGRAIVREPKV
                     FLFDEPLSNLDAKLRVQTRAEITKLHQQLGTTFIYVTHDQVEAMTMGTRIAVMKDGAL
                     QQVDSPQNLYDRPANQFVAGFIGSPSMNFFDVLLKGTRDNVIVEGDSFQLNIPAERTK
                     GLDQYLGQTVSMGVRPEDLHDANFVPPNIHIGTLDAQVDVTELMGNEVYLYLLTGKSS
                     YVARVDPRTKARVGTKVQMAANLDNLHFFSKSNEKAIR"
     gene            1032..1520
                     /locus_tag="VFF70_00495"
     CDS             1032..1520
                     /locus_tag="VFF70_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY43201.1"
                     /translation="MTNEERTRQIEVYGSAVDLLTAALTEFPPEMWQYRSAPDDWTIH
                     EIVIHIADSEANSYVRCRRFIAEPGSMVLGYDERGWADRLRYHEHSTADALQLFKWLR
                     LESYHLIKTLSPVVWANTVNHTESGVMTMDQWLDIYTRHIPDHIAQMRAVYAAWKKSG
                     AH"
     gene            1734..>3083
                     /locus_tag="VFF70_00500"
     CDS             1734..>3083
                     /locus_tag="VFF70_00500"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZY43202.1"
                     /translation="MTSLTSEHHHPLKILFLAAECAPFFKVGGLADVVGSLPQALHEL
                     GHDVRVIMPRYRPIDGAQYGLRRMEGFANVPTADMDRVSEVEVSDAKGLPTYFVWDER
                     FFGRDTVYGQPDEALAFVFFCRAAMEFVRSMDWQPDAIHCHDWHTGAALMMLELNRDQ
                     RFNSIARVFTIHNLVYQGVTGDAIFRFAGFDGHIDRVLGEQAGTANWMARGIAHADMI
                     NTVSPTYAAEIRTPDYGAGLDGLLRARTGHVVGILNGIDRSEWNPATDAALTSKFDAT
                     SIRRRSSNKKVVQETLGLKVNARVPLIGLVTRLAEQKGVDILIAAGERLLERNVQLAI
                     LGSGDERYQTAIDQLRDRYPDQVGVEHRFNDRLARLIYGGSDMFLMPSRYEPCGLGQM
                     IAMRYGSLPIVRATGGLKDSVLDAGRYPKRGTGFSFEPYSPEGLIGAVDRAIKVFANT
                     "
BASE COUNT          664 a          822 c          906 g          691 t
ORIGIN      
        1 tcgcgatatt gccatggtgt tccagtcgta cgcgttgtat ccgcacatga gcgtgtacga
       61 caatctggct ttctcgctgc gactgcgcgc caagaccggc cgcgtgcgca agttggtcac
      121 gctggttttc aatcgaccgg cgtatcaaaa gatcaaagac caggaagcca atattgatca
      181 gcgcgtgcat aaagccgcgc agacgctgct gattgagcag ctgctgccgc gcaaaccaaa
      241 gcaactgtcg ggcggtcaac gccagcgtgt ggccgtgggc cgcgcgatcg tgcgtgagcc
      301 aaaagtcttc ttgttcgacg agccgctgtc caacctcgat gcgaagctgc gcgtgcagac
      361 gcgcgccgag attaccaagc tgcaccagca gctgggcacg acgttcattt acgtgacgca
      421 cgatcaagtt gaagcgatga ccatgggcac gcgtatcgca gtgatgaaag acggtgcgct
      481 gcagcaagtg gattcgccgc agaatctgta cgatcggccg gccaatcaat ttgtggcggg
      541 ctttatcggc agccccagca tgaatttctt cgacgtcctg ttgaaaggca cacgcgataa
      601 cgtgatcgtt gaaggcgata gcttccaatt gaatattccg gctgaacgca ctaagggtct
      661 cgatcaatat ctgggtcaaa ccgtgtccat gggtgttcga ccggaagatc tgcatgatgc
      721 caacttcgtt ccaccgaata ttcatatcgg cactttggac gctcaggtgg atgtcaccga
      781 gttgatgggc aatgaagtgt acttgtattt gctgacgggc aagagctcgt atgtggcccg
      841 cgtcgatccg cgcaccaaag cgcgcgtcgg caccaaagtg caaatggcgg ccaatcttga
      901 caacctgcac ttcttcagca agtcaaacga aaaagcgatt cgttgatcga tctgaattcc
      961 aatagaccct cggcctgaaa cgccgagggt tttgttttag tcccgatcat ttagggcacg
     1021 gagtgctgaa tatgacaaat gaagagcgta cccgccagat cgaagtgtat ggtagcgcgg
     1081 ttgacctgct gacagcggcg ctgacagaat tcccgccgga gatgtggcag tatcgatcgg
     1141 cgcccgacga ctggacgatt catgagatcg tcattcatat cgccgacagc gaagcgaata
     1201 gttacgtgcg ctgccgccgc ttcatcgccg aacccggatc gatggtgctg ggctacgacg
     1261 agcgcggctg ggcggatcgg ctgcgttatc atgagcacag cacggctgac gccttacaat
     1321 tgttcaaatg gctccggctt gagagttatc atctgatcaa gacgctgtcc ccagtggtct
     1381 gggcgaacac cgtcaatcat acggagagcg gcgtgatgac gatggaccag tggctcgaca
     1441 tctatacgcg ccacattccg gatcacatcg cgcagatgcg cgccgtgtat gcggcctgga
     1501 aaaaatcggg cgcacactga tcgctgcagt cttaataaaa tccattgacg aattgtttgg
     1561 ctcgtgctct cgccgctccg gcactgtcac cctgagcgca gcgaagggtc tctatcgatt
     1621 ggcgcagaga ttcttcgctt cggtcagaat gacagttagc gactttgtta aagccctaca
     1681 ctgatcgcag tatttctttg actagccttg cggcttgtgc tagtatgtga tcaatgacct
     1741 ctttaacctc cgaacaccat catcctttga aaattctgtt cctcgccgcc gagtgcgcgc
     1801 cgttcttcaa agtcggcgga ctggccgatg ttgtcggctc gctgccgcag gctctgcatg
     1861 aattgggtca cgacgttcgg gtgatcatgc cgcgctatcg gccgatcgat ggcgcgcagt
     1921 acggcctgcg gcgcatggaa ggtttcgcga atgtgccaac cgctgatatg gatcgcgtca
     1981 gcgaagtgga ggtctccgac gcgaaggggc tgccgactta ttttgtctgg gatgaaagat
     2041 tctttgggcg cgataccgtc tacggccagc ctgatgaagc gctggccttc gtgttcttct
     2101 gccgggccgc catggaattc gttcgatcga tggactggca gccggacgcc attcactgcc
     2161 acgattggca cacgggcgca gcgctgatga tgctggaact gaatcgcgat cagcgcttca
     2221 actcgatcgc gcgggtgttt acgatccata atctggtcta tcaaggtgtg acgggcgatg
     2281 caatctttcg cttcgccggg ttcgatggtc atatcgatcg tgtcttgggg gagcaggctg
     2341 gcaccgcgaa ttggatggcg cgcggcatcg cgcatgcgga tatgatcaac actgtcagcc
     2401 cgacgtatgc cgcagaaatt cgcacgcccg attacggtgc tggcctcgac ggtcttctcc
     2461 gcgcacggac cggacatgtg gtcggaattc tgaacggcat cgatcgatct gaatggaatc
     2521 cggcaaccga tgcggcctta acgtcgaaat ttgatgccac gtcgatcagg cgccgatcga
     2581 gcaacaaaaa agttgtgcaa gagacgctcg gcttgaaagt aaacgcgcgt gtaccgctga
     2641 tcggtttggt gacgcgcctg gctgagcaaa agggtgtcga tattttgatc gcggcgggtg
     2701 aaagattatt agagcgcaat gtgcaattgg cgatcttggg tagtggcgat gagcgttacc
     2761 aaactgcgat cgatcaactg cgtgatcggt atcccgatca agtcggcgtc gagcatcgct
     2821 tcaatgatcg cctcgcccgc ttgatttacg gtggcagtga tatgttcttg atgccgtcgc
     2881 gttatgaacc gtgcgggctg ggccagatga tcgcgatgcg gtatggctca ctgccgatcg
     2941 tccgcgcgac gggcggcttg aaggactcgg tactcgacgc gggacgttat ccaaaaagag
     3001 gcactggctt ttccttcgag ccatattcgc cagaaggcct aatcggggca gtcgatcgcg
     3061 cgatcaaagt atttgccaac aca
//
LOCUS       DASRTJ010000027         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_152015,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000027 DASRTJ010000000
VERSION     DASRTJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152015"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFF70_00505"
     CDS             <1..534
                     /locus_tag="VFF70_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43203.1"
                     /translation="IEAIVDNLLNEIMPDGGWNCRRGGYSKPHHSSFNTTFNVLEGLR
                     EYIEARRGTRRKKAIVAEHSALELMLQHRLYKSDKTGNIINSKFKMLSYPPRWHYDVL
                     RGLVYFARADAPRDDRLQDAIDLLHSRRRTDGLWPVQYKHPAKVFFDMEKTGGPSRWN
                     TLRALQVLRWWNGQPHF"
     gene            complement(651..911)
                     /locus_tag="VFF70_00510"
     CDS             complement(651..911)
                     /locus_tag="VFF70_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43204.1"
                     /translation="MPRIRCNYEGCIHLEGGLCTADEIELDQELGCLTFTQVEEVEIE
                     ELDADLVADVEEEEEEDDDEEWEEELDEDEDDDDDDDDKEEW"
     gene            complement(990..>1910)
                     /gene="glp"
                     /locus_tag="VFF70_00515"
     CDS             complement(990..>1910)
                     /gene="glp"
                     /locus_tag="VFF70_00515"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZY43205.1"
                     /translation="VVPVEDTDDGARGTSSLPSAVSIKKPFQRGAFVRYQGDDVKRGE
                     KILEAGTLIHPADIALMAAVGHARVQVYRQPRVAVLATGDELVEPNQKPDRGQIRNSN
                     EYAVAALIAQSGGLPVRLGIARDNKESIAAKLDQAIGQGVDLIIATAGVSVGAYDFVK
                     DVIASNGSIDLWRVRMRPGKPLAFGNYRSVPFFGLPGNPVSAMLTFEQFVRPVLRVMA
                     GFAHWRKPTIDVVLLESIESDGRETYARAWLQRDADRWTAKLSGGQESNMLKSLARAN
                     ALVIVPDGVTHLERGSIAQAQLLIAFDDIQ"
BASE COUNT          438 a          609 c          436 g          427 t
ORIGIN      
        1 atcgaagcga tcgtagacaa tctcttaaac gaaatcatgc cggatggcgg ctggaactgt
       61 cgacgtggcg gctactccaa accccatcat agttcattca atacaacttt caacgtgctc
      121 gaagggctgc gcgaatatat tgaagcgcgc cgagggacac gccgcaaaaa agcaatcgtc
      181 gccgagcaca gcgcattaga actcatgctg caacatcgat tgtataagtc ggacaagacc
      241 ggcaacatca tcaactcaaa attcaaaatg ttatcctatc cgccgcgttg gcattacgat
      301 gtgctgcgcg gcctcgtcta cttcgcccgc gctgatgcgc cccgcgatga tcgattgcaa
      361 gacgcgatcg atctgcttca ttcgcgacgt cgcacagatg gcttgtggcc tgtgcagtat
      421 aagcatcccg ccaaagtatt cttcgatatg gaaaagaccg gcgggcctag ccgttggaat
      481 acactgcgcg cgttgcaagt attgcgctgg tggaatggtc agccacattt ctgatgatcc
      541 ataaatttaa ttactttcgg ctaggtactc taccaaataa aaatcagcgg actcgatctt
      601 ccaagtccgc tgattcattt catcctcaac actatcgacg actgaagccg tcaccactct
      661 tccttgtcat catcgtcgtc atcgtcatct tcgtcttcgt cgagctcctc ttcccactcc
      721 tcatcgtcat cctcttcttc ctcttcctcg acgtcggcga cgagatcggc atccagttcc
      781 tcaatttcca cttcctcgac ctgcgtgaac gtgagacagc ccagctcctg atcgagttcg
      841 atctcatcgg ccgtgcacag accgccttcc aggtgaatgc agccctcgta attgcagcgt
      901 atcctcggca tcggccaacc tcccgaatca cgaacctctc agagtgtctc gacgagatta
      961 tatcaggaac taatcaacac gcaagcaagt cactgaatat cgtcaaaggc aatcaacagc
     1021 tgcgcttgcg cgatcgatcc gcgctcaaga tgcgtcacgc catccggcac aatcaccagc
     1081 gcattcgctc gcgccaaact cttcagcata ttcgattcct gcccgccact caattttgcc
     1141 gtccaccgat cggcatcgcg ctgcaaccac gcgcgcgcat acgtttcgcg cccgtcactc
     1201 tcgatcgact ccaacagcac cacatcgatc gtcggcttgc gccagtgcgc gaagccggcc
     1261 atcacccgca gcaccggtcg cacaaattgc tcgaaggtca gcatcgccga tacgggatta
     1321 ccaggcagtc caaaaaacgg cacgcttcga taatttccaa acgcgagcgg tttgccgggc
     1381 cgcattcgca ctcgccataa atcgatcgat ccgttcgatg cgattacgtc cttgacgaaa
     1441 tcgtacgcgc ccaccgatac acccgccgtg gcaatgatca gatcaacgcc ttgcccgatc
     1501 gcttgatcta acttcgcggc gatcgattcc ttgttatcac gtgcgatgcc caatcgcacg
     1561 ggcaagccgc cggactgcgc gatcaatgca gccaccgcgt attcattcga gttgcgaatc
     1621 tgtccgcgat ccggtttttg atttggctcg acgagttcat caccggtcgc taacactgcg
     1681 acgcgcggct gtcgataaac ttgcacacgc gcatgtccga ctgcggccat caacgcgata
     1741 tcggccggat gaataagcgt gcccgcctca agaattttct cgccgcgctt cacatcatcg
     1801 ccttgataac gcacgaatgc tccgcgctga aaaggtttct tgatcgagac cgcacttggc
     1861 agcgagctcg ttccgcgcgc accatcatcc gtgtcttcaa cgggcacaac
//
LOCUS       DASRTJ010000028         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_152020,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000028 DASRTJ010000000
VERSION     DASRTJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152020"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFF70_00520"
     CDS             <1..313
                     /locus_tag="VFF70_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43206.1"
                     /translation="DSLTRFQKIEDPAAIAECLFRLAIVFNAKGQTQPAVVLMSAQDS
                     WRAARGTALPPADRAEYDRCRAALRSQLGESNFDAACAEGSTFDLDRAAAYALSIGAQ
                     F"
     gene            376..3432
                     /locus_tag="VFF70_00525"
     CDS             376..3432
                     /locus_tag="VFF70_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZY43207.1"
                     /translation="MDLPLTINLQSKTFSVSPKDRLIDLLRRNGYFGVKHGCDDGSCG
                     ACTVLIDGKPVNSCTLLAAQVEGKTIATIEGVGVMNTSPENGPTHGWKGNVALHPLQK
                     AFVETGAIQCGFCTPAMILAAKSLLDQNQNPSEAEVRDVLSGVLCRCTGYTKPVQAIQ
                     RAAAMMRGEAVQPIELKNEPVPLFNNRMPTNLRDDETGIMVKTTPMPTLVIAPDTQKW
                     KVVGHPEIKVDAYKLVQGKPAFTDDIELRGTLVAKVLWSPIAHGRIKYINVSKARALK
                     GVAAVLTYQDIPRVVYSTAGQSDPIPGPLDAFSLDVKVRFVGDRVAFVAAETEEIAER
                     ALELIEVEYEELPAILDPRESMKPDAIRIHDEPEYVNFADSDPSKNLAAQIRIDIGDV
                     NVGFKQADRIFEADYIVPKVQQVSIETHIVVTYWDEDDRMVIRTSTQVPFHVRRILAP
                     VLGLTAKRIRVIKPRIGGGFGGKQEVMIEDVAAHLTIATGRPVRFEYTRGEEFYAARS
                     RHPMRVHMKTGVTKDGKITANEMVALSDTGAYGCHALTVTGNTGHKGMALYTGQPDLL
                     PNPAPFANDANKGGNAAVPNIRFYADVVYTNTPPAGAYRGYGVPQGAMPVDVHMERIA
                     HEMKIDPIEFRLKNIVRAGDEHPFSRAWSEGREPRPEIIKTNALPECVAQASATIDWV
                     RKFGNPDWHTVPNKPHLRRGIGIACVMQGTAIPYLDMGAASIKMNDDGSFNVLVGATD
                     LGTGSDTVLGQMAAEVLGVPLDDIIMYSSDTDFTPFDKGAYASSTTYISGAAVVKAAE
                     KVAEQIKQVASEMMQVSVDQIHLADKKAWGPDGRSATLEKIALRSLHAENQHQIMAVG
                     SYMSPESPPPFAAQFAEVTVDTETGQVTVDRLVMAIDSGVIVNPVTASGQIEGGMTQA
                     LGYAVCEEMKYDEKGRSLANDLYTYKIFRADEMPALDVIFVETMEPSHPFGVKAVAEI
                     PMDGVAPAVVNAIHDATGAWLNEIPTTPERVWRTMHSDQLTMNNDK"
     gene            3593..3883
                     /locus_tag="VFF70_00530"
     CDS             3593..3883
                     /locus_tag="VFF70_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZY43208.1"
                     /translation="MTDNIYSAILEAQSKGIASALCTVIKSSGSVPRHESSKMLVYLD
                     GRFVGTVGGGEMESRVIEAAKSIARSGEAKIVEYSLVDPKQGDPGVCGGTVE"
     gene            3917..4396
                     /locus_tag="VFF70_00535"
     CDS             3917..4396
                     /locus_tag="VFF70_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZY43209.1"
                     /translation="MIFGAGHVGRATAHLAKWLGFRVIVADDRSDLASADWIPEADQY
                     VTGSINEQLDQININSQTYILSLTRGVMIDIETLPALLNTDATYIGVIGSRRRWQTAL
                     KQLREQGVSEEKLNRVHAPIGLELNAETPEEIATSIMAEVVMLKNKGSGKSMMTSDT"
     gene            4462..>4711
                     /locus_tag="VFF70_00540"
     CDS             4462..>4711
                     /locus_tag="VFF70_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZY43210.1"
                     /translation="MWQTYYTATTIDDVLKLLTQHGKNARLIAGGTDILIELERKIRT
                     PSVLIDITHIPGLDQIKLKKNILHLGPLVTHNQVVGSKE"
BASE COUNT         1178 a         1233 c         1313 g          987 t
ORIGIN      
        1 ggatagcttg acgcgctttc aaaaaattga agaccccgcg gccattgccg aatgtttgtt
       61 tagactggca atcgttttca acgccaaggg acaaacacag ccggcggtcg tgctcatgag
      121 cgcgcaagat tcttggcgcg ccgcccgggg cactgccttg ccgccggccg atcgggccga
      181 gtacgatcga tgccgcgcgg ccttacgcag ccaattaggc gaatcaaatt tcgatgcagc
      241 ctgcgcagag ggtagcacgt ttgatttaga tcgagctgcc gcttatgcct tgagtatcgg
      301 cgcgcagttt tgagttcgac ctgacagaac tagccgacaa atggaagaca atactgataa
      361 agtggattag cgatcatgga cttaccatta acgataaact tgcaatcaaa aactttttca
      421 gtttctccca aagaccgctt gatcgatctg ctccgccgga acggttattt cggcgtgaag
      481 cacggctgcg atgacggatc gtgcggcgcg tgtacggtat tgatcgatgg caagccagtt
      541 aattcttgca cgctgctcgc cgcacaagtg gaaggcaaga cgatcgccac cattgaaggt
      601 gttggggtga tgaatacgtc gcccgaaaat ggaccgacac acggttggaa gggcaacgtc
      661 gccttacatc cactgcaaaa ggcctttgtc gaaacaggcg cgattcaatg cggcttctgc
      721 acacccgcga tgatcctcgc cgccaaatca ctgcttgatc aaaatcaaaa tccgtctgaa
      781 gccgaagtgc gcgatgtatt gagtggagtg ctatgtcgat gcacgggtta caccaaaccc
      841 gtgcaagcaa tccaacgcgc agcggcgatg atgcgcggtg aggcagtcca gccgatcgag
      901 ttaaagaacg agcctgtgcc acttttcaac aatcggatgc ctactaatct gcgcgatgat
      961 gaaacgggaa taatggtgaa gactacaccg atgcccacgc tcgtcattgc acccgatacg
     1021 caaaaatgga aagtcgtcgg ccatccggaa atcaaagtcg atgcgtacaa attggtgcag
     1081 ggcaaacctg cctttactga tgacatcgaa ttgcgcggga cgctggttgc taaagtattg
     1141 tggtcgccga tcgcgcatgg ccgcatcaaa tatatcaatg tcagtaaagc gcgtgcgttg
     1201 aaaggtgtag cggccgtcct cacgtatcaa gatattccgc gcgtcgtgta ttccaccgcc
     1261 gggcaatccg atccgatccc cggcccgctc gatgcgttct cgctcgatgt caaagttcga
     1321 ttcgtcggcg atcgcgtggc ctttgtagct gccgagaccg aagagatagc ggaacgcgcg
     1381 ctggaattga tcgaagttga atacgaagag ttgccggcca tcctcgatcc gcgcgagtcg
     1441 atgaagccgg acgcgatccg tattcacgac gaacctgaat acgtgaactt tgcggattcc
     1501 gatccaagta aaaatctcgc ggcgcaaatc cggatcgata tcggcgacgt gaacgttggg
     1561 ttcaagcaag ccgatcgtat atttgaagcc gactacattg ttcccaaggt gcagcaagtc
     1621 tcgatcgaaa cacacatcgt tgtcacgtat tgggacgaag acgatcgcat ggtcattcgc
     1681 acttcaacgc aagttccttt tcatgtcaga agaatcctcg cgccagtgtt gggcttaacc
     1741 gccaagcgca tccgcgtgat caagccgcgc atcggcggcg gcttcggcgg caagcaggaa
     1801 gtgatgatcg aagatgtggc cgcgcatctc accattgcga ctggtcgtcc ggttcgattt
     1861 gaatatacgc gcggtgaaga attttacgcg gcgcgatcaa gacatccgat gcgcgtccat
     1921 atgaagacgg gtgtgacgaa agacggcaag atcactgcga atgaaatggt tgcgttgagc
     1981 gacactggcg catatggctg tcacgcgctc acggttacgg gcaatactgg acacaaaggg
     2041 atggcgttgt acacgggtca acccgacctc ctccctaacc ctgccccgtt cgcaaacgac
     2101 gcgaacaagg gagggaacgc ggcagttccc aatattcgtt tttatgcgga tgtggtgtac
     2161 accaacacac cgcccgcggg cgcctatcgg ggttatggtg tgccgcaggg tgcgatgcca
     2221 gtcgacgtgc acatggagcg cattgcacac gaaatgaaga tcgatccgat cgagtttcgt
     2281 ttgaagaaca tcgtccgggc gggggatgag catcccttca gccgcgcatg gagtgagggc
     2341 cgcgaacctc gaccggagat catcaagacg aatgcgttgc ccgaatgtgt ggcgcaggct
     2401 tcggcgacga ttgactgggt gcgcaagttc ggcaatcccg attggcatac tgtgcccaac
     2461 aagccgcatc tgcggcgcgg catcggcatc gcgtgcgtga tgcagggcac tgcgattcct
     2521 tatctcgaca tgggcgcggc ctcgatcaag atgaatgatg acggatcgtt taatgtgtta
     2581 gtcggcgcga ccgatctcgg aactggatcg gatacagtac tgggacaaat ggccgccgag
     2641 gttctcggcg tgccgctcga tgacatcatc atgtattcaa gcgacaccga tttcacaccg
     2701 ttcgataaag gcgcatatgc ttcatccacc acctatatct caggcgcagc cgtggtcaag
     2761 gccgccgaaa aagttgcaga acagatcaag caagttgcaa gtgaaatgat gcaagtttca
     2821 gtagatcaaa ttcacttggc agacaaaaaa gcgtgggggc cggatggccg atcggctacg
     2881 cttgaaaaga tcgcactgcg atcgctgcat gcggagaatc agcatcagat catggcggtc
     2941 ggatcgtaca tgtcgcccga atcgccaccg ccgttcgccg cacagttcgc ggaagtcacg
     3001 gtcgatactg aaaccggcca agtcacggtc gatcgattgg tgatggcgat cgattccggc
     3061 gtgatcgtca atcccgtcac ggcatccggc caaatcgaag gcggcatgac gcaagcactt
     3121 ggttatgcag tgtgcgaaga gatgaagtat gacgagaagg gccgatcgct cgcgaacgat
     3181 ctgtacactt acaaaatttt ccgcgcggat gaaatgcccg cgctcgatgt gatcttcgtc
     3241 gagacgatgg agccgtcgca tccgttcggc gtgaaggccg tcgctgaaat cccgatggac
     3301 ggtgtcgcac ctgcagtggt caacgcgatc cacgacgcaa ccggcgcatg gctgaatgaa
     3361 attccgacta cgccggagcg agtgtggcgg acaatgcaca gtgatcaatt aacaatgaac
     3421 aatgacaaat gacgagctag cgtggcaggt tgtcatttcg agccgcgtaa atcgcggaga
     3481 gaaatctctg cccctgttaa cacaattgtc cactgtgata gagatttctc gcttcgctcg
     3541 aaatgacaca ggttgattgc aagttgcccc gaatcaagta caatcatcca cgatgactga
     3601 caacatctat tccgctatcc tcgaagcaca atcgaagggc attgcatccg cactctgcac
     3661 cgtgatcaaa tcgagcggct cggtaccgcg gcatgaatca tcgaagatgc tggtctatct
     3721 cgatggacgc ttcgtcggca cagtcggtgg cggcgaaatg gaaagccgcg tgatcgaagc
     3781 ggccaaatcg atcgcccgat ccggcgaagc gaagattgtt gaatacagcc tcgtcgatcc
     3841 gaaacaaggc gatcctggtg tatgtggagg cactgtggaa taatttatcg agccaatcaa
     3901 tcccccagcc accatattga ttttcggcgc aggccatgtc ggtcgagcga cggcgcactt
     3961 agcgaaatgg ctgggcttcc gcgtgattgt tgccgacgat cgatcggatc tcgcttcagc
     4021 cgactggatt ccagaggccg atcagtatgt gactggctca atcaatgagc aacttgatca
     4081 aatcaacatc aattcacaga cctatattct gtcactcacg cgcggtgtca tgatcgatat
     4141 tgagactttg cctgcgttgc tgaatacaga tgccacatac atcggcgtga taggttcgag
     4201 acgacgatgg caaacggcct tgaagcaatt gcgcgagcag ggcgtgagcg aagagaaact
     4261 taatcgcgtg catgcgccga tcgggttgga actcaacgcc gagacgccgg aagagatcgc
     4321 caccagcatc atggcggaag tggtgatgct gaaaaataag ggttcgggga agtcaatgat
     4381 gacgagtgat acgtgagcag cttatcatgc atcacgtatc aggcatcaag ttagcaaatc
     4441 atttcacctg gaatatttat catgtggcaa acctactaca ccgcaacaac catcgatgac
     4501 gtattaaaat tgctgacaca gcatggcaag aacgcgcgct tgatcgccgg cggcaccgac
     4561 attttgatcg agttggagcg caagatcaga acgccgagtg tgttgattga tatcacgcat
     4621 attcccggcc tcgatcagat taaattgaag aaaaacatcc tccaccttgg cccgctcgtc
     4681 acccataacc aagtcgtcgg atcgaaagaa c
//
LOCUS       DASRTJ010000029         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_228523,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000029 DASRTJ010000000
VERSION     DASRTJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228523"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VFF70_00545"
     CDS             complement(<1..115)
                     /locus_tag="VFF70_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZY43211.1"
                     /translation="MSDLGSQLRAAREAKGLSLEQVFKATRIKLSYLEALES"
     gene            complement(196..672)
                     /gene="rplI"
                     /locus_tag="VFF70_00550"
     CDS             complement(196..672)
                     /gene="rplI"
                     /locus_tag="VFF70_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZY43212.1"
                     /translation="MKVLLTHDVTKLGKAGMVKNVTDGYARNYLLPQGMAVLATAGAV
                     KQSESLSKAEQKRQSVLATDASALAEQLQAVSLTFHMRAGEDGKLYGSVTASNIVDEL
                     KRTTGLELDRHKLELREPLRSLGAHKVAVRLASELAPEMTVHVVREETGSTEPAAA"
     gene            786..1586
                     /locus_tag="VFF70_00555"
     CDS             786..1586
                     /locus_tag="VFF70_00555"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HZY43213.1"
                     /translation="MTLDTPPLPASPWQRLPNALSSAVLLAIVGLFFIAAPPDLLDKL
                     NLIGFALCHRLPERSFVIDGHQLPLCARCTGIYLGLLIGTLSLIVRRRTRASRLPATP
                     IIVALIGFIVIMGIDGLNSTISVIPNAPQLWHTTNFLRITTGTLYGLAISMLFPPFFN
                     LAVWSQPTGNRTLKSWRELLPMLIVAAVAITIVQAKPDWLLYPIALISIGGALALLSL
                     INSIFVLSAMKLENAIGRWQQLARPLLFGLALGLIEITLLDLLRAASL"
     gene            1707..>2126
                     /locus_tag="VFF70_00560"
     CDS             1707..>2126
                     /locus_tag="VFF70_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43214.1"
                     /translation="MQGGNMQFKFDQAGLMKVGLLPIIAAAIAGALYIVFGLWAIAGI
                     LSWVAFVFGGVWYVMSLRKAGTTPDLVNLLVNAAILGAVAGLVYGIVEMITIPMGLNS
                     ALGGLGGLGAFGAGAVGIGTVISEIIRGAIVAAGAAWV"
BASE COUNT          408 a          591 c          586 g          541 t
ORIGIN      
        1 tcgattcgag tgcttcgaga taggatagtt tgatgcgggt agccttgaag acttgttcca
       61 gactcaagcc tttggcctct cgcgccgcgc gaagttgaga accgaggtca ctcatggggt
      121 ttcaatcaac cacgatcgca cggatgacac gaatgaattg ctcggcgtgt ttctgcgtgt
      181 tctgcgtgga tcgaattaag ccgcggcagg ttcggttgag ccggtctctt cgcgcaccac
      241 atgaacggtc atctcgggcg cgagttcgct ggccagacgc acagcaactt tgtgcgcgcc
      301 gagtgagcgc agcggttcac gcagttccag cttatgccga tccagttcaa gaccggtggt
      361 gcgcttgagt tcatcgacaa tgttggacgc cgtcaccgag ccgtacaact tgccgtcttc
      421 gcccgcgcgc atatggaacg tcaatgacac cgcttgtagc tgctcggcca atgcggacgc
      481 atcagtggct aacaccgatt ggcgcttctg ctccgctttg gacagcgact cagactgttt
      541 cactgcgccg gcagtggcta gcacggccat cccttgcggc agcaaataat tgcgggcata
      601 accgtcggta acattcttga ccataccggc cttgcccaac ttggtcacat cgtgggtcaa
      661 taatactttc atcgtcagct tactccctca aagattcgga ttcagatgat acctcaaaaa
      721 cgtcgaaaac tcaactggct gatcagcgtg ttataatcaa atcacttggc ggggtaagga
      781 ttgatatgac acttgataca ccgcccctgc ccgcctcgcc gtggcaacgc ctgcccaacg
      841 ctttgtcctc cgcggtgctg ttggctatcg tcggcttatt cttcatcgcc gcacctcctg
      901 acctgctcga caaactgaat ctcatcggct tcgcgctttg tcatcgcctg cccgaacgtt
      961 ccttcgtcat cgatgggcat caactgccgc tgtgcgcgcg ttgcaccggc atatacctcg
     1021 gcctgttgat cggcacgctg tccttgatcg tccgtcgtcg aacgcgcgcc tcgcgcttgc
     1081 ccgctacgcc gataatcgtc gcgctgattg gtttcattgt catcatgggt atcgacggtc
     1141 tcaactcgac gatctcggtc atcccgaatg cgccacagtt atggcatacc accaacttcc
     1201 ttcgaatcac aacgggcacg ttgtacgggc tggccatcag tatgttgttc ccgccttttt
     1261 tcaatctagc tgtgtggagt cagcccacgg gcaatcgcac gctcaagagt tggcgtgaac
     1321 tgctgccgat gttgatcgtc gccgctgttg cgattacgat cgttcaagct aaacccgatt
     1381 ggctgctcta tccgatcgcg ctgatctcga tcggcggagc gttagctttg ctatcgctga
     1441 tcaattcgat cttcgtgctg agcgcgatga agctggagaa tgcgatcggg cgttggcagc
     1501 aactcgccag gccgctgctc tttggattgg ccctgggcct gatcgaaatt acactccttg
     1561 atttgctgcg agcggcttcg ttgtaaagat gcgcgcctca attccgcacc atttaggctg
     1621 gccgtgacag tccggttcgg atttgcatat ttaggtgatt ctggtctaca ataggccatc
     1681 ctaaatccct ttcaggagga gtcaaaatgc aaggtggaaa tatgcagttc aagtttgatc
     1741 aggcaggatt gatgaaggtc ggactattac cgataattgc agcagcgatt gcaggcgcac
     1801 tctatatcgt cttcggcttg tgggctattg cgggaatttt gagctgggtc gccttcgtgt
     1861 ttggcggcgt ctggtacgtg atgagcctgc gcaaagctgg cacaacaccc gatctggtga
     1921 acttgctcgt taatgccgcg attctcggcg ctgtcgctgg actggtgtat ggcatcgtcg
     1981 aaatgatcac gatcccgatg ggtctgaatt cagctcttgg tgggttaggc ggtctaggcg
     2041 cgtttggcgc aggtgcagtg ggcatcggca ccgtcatctc ggaaatcatc cgaggtgcaa
     2101 tagtggctgc tggcgctgct tgggtc
//
LOCUS       DASRTJ010000030         9032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_304359,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000030 DASRTJ010000000
VERSION     DASRTJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304359"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFF70_00565"
     CDS             <1..377
                     /locus_tag="VFF70_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43215.1"
                     /translation="IYRVLKPGGMIVVHTAPNRWYDRYAYPFVRFIRRLQGKKYPSNP
                     RALNVEANVDVHVNEQDIRSMKRYLRRAGFHQVNVWLHSPPQNRHENILFRAARHIAF
                     EWLPFRWFFEREVFAVGVKNDK"
     gene            423..1376
                     /locus_tag="VFF70_00570"
     CDS             423..1376
                     /locus_tag="VFF70_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HZY43216.1"
                     /translation="MPDRRSVDELSIEELEEILAIRKREAREARLERLRGEGRVIELA
                     DLKDEPIQSDRPAGEALKPSSSYYRSGEVVDERSSRWRLKLKLSRSSVKTWRDRFLLL
                     VEIGAVIGLIAVIIATEQSRIATNQTVITDIVTPVPTPLIAAVVLPSGHRPPTAPGGA
                     APNLLDEVPAHLRDYVQSITPLPIPTPGPGQPTRIQIPAINIDAQVVAGDDWEQLKKG
                     VGHHPGTANPGERGNVVLSAHDDIFGEIFRHLDQLKPGDEVIVYSGNQRYRYVITAQR
                     IIRPTQVEVMQPTSDPTVTLISCYPYLVDTQRIAVFAQLAQ"
     gene            1481..1744
                     /locus_tag="VFF70_00575"
     CDS             1481..1744
                     /locus_tag="VFF70_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43217.1"
                     /translation="MAKKTRKVKQQESQQRAIRQAVNNTQSVSIAPMAGAAPVMVNRS
                     AGVRAAPLEPGEEYKYVRSDLKRIALLALSFTVILIALSFVIK"
     gene            complement(1822..2544)
                     /locus_tag="VFF70_00580"
     CDS             complement(1822..2544)
                     /locus_tag="VFF70_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43218.1"
                     /translation="MTDAKLLPIIWTLASMVIAVLITLIFSPARADRFRSTLAGRLLE
                     HIFRLIYFIGLPYAALLTHSLSAVDLGFMGNSGSNEAAVFGWTSADWLRGLSTGLTLG
                     LIVLIPIGLAARQMARAGSPLGVDVRSAGSIAIDSVYSEAHWAFYRSAPLILLGSAYG
                     AALIGLLLVSIELVVAMVRNGLGTQPEDRQSWIGQALLLAMSATTFILTRNVWLAIAL
                     HMVIEVALKAITTRLAAHMKIA"
     gene            complement(2541..4238)
                     /locus_tag="VFF70_00585"
     CDS             complement(2541..4238)
                     /locus_tag="VFF70_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZY43219.1"
                     /translation="MRPSSSRNSKIDWRLAVLRWVLIFGALAVALLASGSAGELGRRS
                     LALLVVTAVYNLVIMILLIFDVRIDGLPWISLAIDTVLAIVLLFVSGGSNSPLLFFSL
                     FPILTAALRFSWIISALTTATIAIAYLALEGPLPSVILIVSVAIFSAAISSRMRDIVR
                     DNRDTADDDELQQLRSTREQARAIFEMASTLSATLNYQHILDSVLDVSVLGLKEIGPT
                     STRMVGIVLLFAGSGLRVAASRHLDMFDEKLQLELRGGAISDALASAEPVICHDTNRD
                     PEISTYTALHRCKIAVAVPLRAGFENLGVALFGSQDAGLFTPEHIDLLLAVCNQATIA
                     LQNAQLFESLRAEKARIVEIEEDARKKLARDLHDGPTQSVAAIAMRLNYTRTLFQRDP
                     VRVPEELEKLEEMARNTTKEIRHMLFTLRPLVLETQGVIAALETLVQKLHDAEPTLNV
                     QLLLDDQLENLDKDAQGMIFYVVEEAVGNARKHASAKNVWVRTAPDGKDYILEIKDDG
                     IGFDVGKVQANYDKRGSLGMVNMSERAEVMSAKLSIASAMGQGTRVTMRMPLGRVQK"
     gene            complement(4616..4689)
                     /locus_tag="VFF70_00590"
     tRNA            complement(4616..4689)
                     /locus_tag="VFF70_00590"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4653..4655),aa:Val,seq:cac)
     gene            4962..5261
                     /locus_tag="VFF70_00595"
     CDS             4962..5261
                     /locus_tag="VFF70_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HZY43220.1"
                     /translation="MDIRQLSEEMQRFVEAKGWTRSDSPRPQTPRNIATSLAIEAAEV
                     LEHFQWEDRVIDRPELAAELADVMLYLLQLASISGIDLEQAVLGKLKVNYDRTWD"
     gene            5242..5802
                     /locus_tag="VFF70_00600"
     CDS             5242..5802
                     /locus_tag="VFF70_00600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZY43221.1"
                     /translation="MTELGTDQKPWQVLNKRTIYRSDWINLDQWSVRLPDGSIIPDHH
                     VLDYPYEAVGIIPIGADGRILMIDHYRFITDTRGWEIPSGGIEQGESVLQSAQRELLE
                     EAGYSASDWKVLGKYHPSNGSSNQVFHAAIARQLNRESEPTDVNETLGLRWFTIDQVR
                     QLIDRNGIRDGLSLTTLCWGMIVGLT"
     gene            5799..6440
                     /locus_tag="VFF70_00605"
     CDS             5799..6440
                     /locus_tag="VFF70_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZY43222.1"
                     /translation="MKILAISDQVEDALYKPSIVDRFSDIDLVISCGDLPYSYLEYIV
                     TVLNKPMYYVHGNHDLTLEYTESGAQRLIPAGVESLNSRTTIGPQGMLLAGLEGSIRY
                     DPRSAFQFTQSEMNLKAWRLSLRLIRNQLRYGRYLDVLVTHSPPFGVGDGPDYAHIGF
                     KAFNRLIKRYRPKLLLHGHQHTYLGLKPGTLVEATNVFNVFPYRVIEWEYGDE"
     gene            6433..7389
                     /locus_tag="VFF70_00610"
     CDS             6433..7389
                     /locus_tag="VFF70_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HZY43223.1"
                     /translation="MSDDGRAQNEYERMRRRTLFRDVLARLAGRPNRLLSYEEVKQSL
                     ELGGPIYRGLKPVPVVQIVGSVDRYRDFDAEFMPAQDRTADRWKSVARAYYDEVNLPP
                     VRLYKVGDAYFVLDGHHRVSVAREQGVAFIDAEVLEAVSRVPVSADLRAEDLEVLHEY
                     RHFLERTRLDEIRPDQHIRFTVAGGYDRLIEHMAMHRYFIQVEWGRDVGEEQAVAHWY
                     DTVYMPVVEAIRAHNVLADFPHRTESDVYLWIVEHLYYLQEKEKDVSIEEAAEEYADQ
                     FSERPIKKIVRSVKQAFEGTETRSDSEAQKSNGMESEKNVTT"
     gene            7376..7912
                     /locus_tag="VFF70_00615"
     CDS             7376..7912
                     /locus_tag="VFF70_00615"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZY43224.1"
                     /translation="MSQLKRSIVTVFGTNAPKAGEPDYEQARALGAVLAKAGYVVATG
                     GYFGTMEAASRGAKEAGGHVIGVTSSVFDDLRLTANPYLDEEIKFPTLFQRLHHLVTF
                     ADAWVALPGGIGTLSEVALTWSLMQVGEKSRQPFVLVGSMWRDLLENFSKNGYVKEEY
                     HQLIRYADDIDAVLGLLK"
     gene            8151..>9032
                     /locus_tag="VFF70_00620"
     CDS             8151..>9032
                     /locus_tag="VFF70_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZY43225.1"
                     /translation="MADKLTPRSEDYSKWYNDLVQRAELADYSPVRGSMVIRPYGYGL
                     WENVQAGLDRRFKATGHQNAYFPLLIPMSFLQKEKEHVKGFAPELAVVTHAGGEKLEE
                     PLVIRPTSETIIGHMYAQWIKSYRDLPILINCWNNVVRWELRTKLFLRTAEFLWQEGH
                     TAHATAEEAEEETIRMLNVYADFAVNDAAIPVIPGRKSDSEKFAGAVRSYTIEAMMGD
                     KKALQSGTSHFLGQNFAKAFEIKYLSKNNAQEFVWTTSWGLSTRMIGGIIMVHGDDQG
                     LKLPPKLAPIQVVIVPIF"
BASE COUNT         2102 a         2330 c         2567 g         2033 t
ORIGIN      
        1 acatctatcg cgtgttgaag ccgggcggca tgatcgtcgt ccataccgcg cccaatcgct
       61 ggtacgatcg atacgcttat ccatttgtgc gtttcattcg ccgtttgcag ggaaaaaagt
      121 atccgtcgaa tccgcgcgcg ctcaacgtcg aagcgaatgt cgacgtgcat gtgaatgaac
      181 aagacattcg cagcatgaag cgttacttgc gccgcgcagg ttttcatcaa gtaaacgtgt
      241 ggctgcatag tccaccgcag aatcgacacg aaaacatttt gtttcgagcg gcacgtcata
      301 tcgcctttga gtggcttccg tttcgctggt tctttgagcg cgaagtgttt gcagtaggag
      361 tcaagaatga taagtgacgg gtgattcgtt tctcacccgt cacttattac ttgtcactgg
      421 ctatgcctga tcgtcgcagc gttgatgaac tttcaattga agagctggaa gaaattctgg
      481 cgattcgcaa acgcgaggcg cgtgaggctc gcttggagcg gctgcgtggc gaaggccgcg
      541 tgatcgagct ggccgatctc aaagacgagc ctatccaatc cgatcgacct gctggcgaag
      601 cgctcaagcc gtcgagcagc tattatcgat cgggtgaagt ggtggacgaa agaagctcac
      661 gctggcgctt gaagctaaag ctcagtcgat cgagcgtgaa gacgtggcgc gatcgattct
      721 tgctgctggt ggagatcggt gcggtgatcg gtttgatcgc cgtgatcatc gccacggaac
      781 aatcgcgcat tgccacgaat cagaccgtga ttacagatat tgtaacgcca gtgccgacac
      841 cgttgatcgc tgcggtcgta ttgccatctg gccaccgtcc gccgactgcg ccgggcgggg
      901 cggccccgaa tttgctcgat gaagttcccg cgcatctgag agactacgtg caatcgatca
      961 cgccgctgcc gatcccgacg cccggccctg gccaaccgac gcggattcaa atcccggcga
     1021 tcaatatcga tgcgcaggtg gttgccggcg atgattggga acagttgaag aaaggcgtgg
     1081 ggcatcatcc gggtacggcc aatccgggcg aacgcggtaa cgtggtgcta tcagcccatg
     1141 atgacatctt cggtgaaatt ttcagacacc tcgatcaact caagcccggc gatgaagtga
     1201 ttgtgtattc gggcaatcaa cgctatcgat atgtcatcac ggcgcagcgc atcattcggc
     1261 cgacgcaggt cgaagtgatg cagccgacca gcgatcccac tgtgacattg atttcttgct
     1321 atccgtattt agtcgataca cagcgcattg ctgtctttgc gcaattggcg caataaaaac
     1381 gcgtgaacaa actgccggaa cgaagtgaat ggtctgaacc ttcgccagga tatctgactg
     1441 tggtagaata gcgcctatcc cttcaagagg aatttgattc atggccaaga agactcgcaa
     1501 agttaaacaa caagaatccc aacagcgcgc gattcgacag gcagtcaaca acacgcagtc
     1561 cgtttcgatc gcgccgatgg cgggcgctgc gccggtgatg gtaaatcgat cggccggtgt
     1621 aagggccgct ccgctcgaac cgggcgaaga atacaagtat gtccgatcgg atctcaagcg
     1681 tattgcactg ctcgctctat cgttcaccgt gatcttgatc gcgctgtcgt tcgtcattaa
     1741 gtaagacaac tcgcccgaag aattaaaaag gatcgacgcg aaatcgtcga tcctttttcg
     1801 ttttagtttt gttccgatcg attaagctat cttcatgtgc gctgccagcc gggttgtgat
     1861 cgctttcagt gcgacttcaa tgaccatgtg cagtgcaatc gccagccaga cattacgtgt
     1921 caagataaac gtagtggcgc tcatcgccaa caacagcgct tgcccgatcc acgattgccg
     1981 atcttccggc tgcgtgccca aaccgttccg caccatcgcg acgacgagct caatgctgac
     2041 caacagcaag ccaatcaacg ccgcgccata tgcactgcct aacaagatca gcggagcgga
     2101 tcgatagaac gcccaatggg cttcgctata aactgaatcg atcgcgattg atccggccga
     2161 tctcacgtcg acacccaacg gcgatccagc gcgagccatt tgtcgggcag ctaacccgat
     2221 cgggatcagc acgatcaagc ccaacgtcaa cccggtgctg agtccgcgca gccaatcggc
     2281 ggatgtccag ccgaagaccg cagcctcgtt cgatccgctg ttgcccatga aacccagatc
     2341 gacggccgat agggagtgtg tcaacagtgc ggcgtagggc agaccgatga agtagatcaa
     2401 acggaagatg tgttcgagga ggcggccggc aagtgtggaa cgaaatcgat cggcgcgggc
     2461 ggggctgaag atcaaagtga tcagcactgc gatgaccatc gaggccagtg tccagatgat
     2521 cggcagcagc ttggcgtcgg tcatttctgc acgcgcccga gcggcatccg catggtgacg
     2581 cgtgtgcctt gacccatggc agacgcgatc gaaagtttgg cgctcatgac ttcggcgcgc
     2641 tcgctcatgt tgaccatacc cagactgccg cgcttgtcgt agttggcttg aactttgcct
     2701 acgtcaaagc cgatgccgtc gtctttgatt tcgagaatgt agtccttgcc gtccggcgca
     2761 gtgcgcaccc agacgttttt cgcgctcgcg tgtttgcgcg cattgcccac ggcttcttcg
     2821 accacgtaga agatcatgcc ctgcgcatct ttgtccaggt tttcaagctg atcgtccaac
     2881 aatagctgca cgttcagggt aggttcagca tcatgcagtt tctgcacgag tgtttccagc
     2941 gcggcgatga cgccctgcgt ttccagcacc aagggccgca gggtaaacag catatgtcga
     3001 atctctttgg tggtattgcg cgccatttct tcaagttttt ccaactcttc cggcacgcgc
     3061 accggatcgc gttggaacag cgttcgggtg taattcagcc gcatcgcgat ggcggccacg
     3121 ctttgggtgg ggccgtcgtg cagatcacgc gccagttttt tgcgggcgtc ttcttcgatc
     3181 tcgacgatgc gggctttctc ggcgcgcagg ctctcaaaca actgagcgtt ctgcaaagct
     3241 atcgtcgctt gattgcagac tgccagcagc agatcgatgt gctccggtgt aaagaggccg
     3301 gcatcttgac tgccgaataa ggccacgcct aaattttcga agccggcccg cagcggcacg
     3361 gccacagcaa tcttgcagcg atgcagcgcg gtgtaagtgc tgatctcagg atcgcgattt
     3421 gtatcatggc agatgactgg ctcggcgcta gctaaagcgt cagagatcgc accgccgcgc
     3481 aattcaagtt gaagtttttc gtcaaacata tctaaatgtc gcgaggcggc cacgcgcaaa
     3541 cctgaaccgg cgaataacag cacgatgccg accatgcgag tggaagtcgg cccgatttct
     3601 ttgaggccca ggacgctgac gtccagcacg gaatcgagaa tgtgttgata gttgagggtg
     3661 gcgcttaagg tgctggccat ttcaaaaatg gcgcgcgcct gttcgcgggt cgagcgcagc
     3721 tgctggagtt catcgtcatc tgcggtgtcc cgattatcgc gtacgatgtc ccgcattcgg
     3781 ctgctgatgg cggcggaaaa gatcgccacg gacacgatca ggatgacgct gggcagcggg
     3841 ccttccagtg ccaaatatgc aatggcgatc gtggccgtcg ttaacgcact gatgatccag
     3901 ctaaaacgca gtgccgcggt gaggatggga aacaaactaa agaataacaa ggggctattg
     3961 ctgccgccgc taacgaataa caacacaatg gcgagcacgg tatcgatggc gaggctgatc
     4021 cacggtagcc catcgatgcg cacgtcgaag atcagcaaaa tcatgatcac cagattgtac
     4081 acggcggtga ccaccaacaa cgccagcgat cggcggccca gttcacccgc actgccagag
     4141 gccagcaggg ccacagctaa tgcgccgaag attaaaaccc agcgcaggac ggccagtcgc
     4201 caatcgatct ttgagttgcg agatgacgac ggtcgcatct tatccggaac tccatcgagg
     4261 aaagtgggct aaactgcggc gattgtacca caagtcatat ggggaaaatt tagtgagacc
     4321 gctacttctt ttgcccgtcg cggaaaatct acagcaattc tgcttgcgag gtggcatccc
     4381 gctcggtcaa ttagcccagc accccgatgg tctgctttcg agcagcacga tgtgcggtga
     4441 gaaatatcag attcaatgag cagcagatga tgaatggaca cagagatttc ggccacatca
     4501 atgctccagc ggcgcatcga tggcccgcta agcgtcgcta cgcttgaaat gatagtgaca
     4561 gatgcaataa caaacggcca tcgatcaaat cgatggccgt ttcatttggg atgagtgggc
     4621 ggtataggac ttgaacctac gaccccacgg atgtgaaccg tgtgctctaa ccagctgagc
     4681 taaccgcccg aagtgtttga gattatagca tcgcgctgat caattgacaa gtgttttgaa
     4741 aaaaagtata atgccgccca tgttcattcg caaagttttg ctaagcccgt tgcctactga
     4801 gccgaaccac aagtctggat aggcgtattg cagctgtcgc ttcgagtttg caaccagtcc
     4861 tctccggctt tgagggggct ggttttttat tgccgagatc ctcccgcagg tttcaggcaa
     4921 tctattgaga acccaagttc aacctgcggg aggatcatcg aatggatatt cgtcaattgt
     4981 ctgaagaaat gcagcgcttc gttgaagcga aaggctggac gcgatccgat tcgccgcggc
     5041 cgcagacgcc gcgcaacatt gcgacttcat tagcgatcga agcggccgaa gtgctggagc
     5101 actttcaatg ggaagatcgc gtcatcgatc ggcccgagct ggccgccgaa ctcgccgatg
     5161 tgatgttata tttactgcaa ctcgcatcga tcagcggtat cgatttggaa caagcggtgc
     5221 tgggtaagtt gaaagtgaac tatgaccgaa cttgggactg atcaaaagcc ctggcaagtc
     5281 ttgaataaac gcacgattta tcgcagcgac tggatcaatc ttgatcaatg gtcagtgcgc
     5341 ttgcccgatg gatcgatcat tcctgatcat cacgtgctgg attatccata cgaagcggtc
     5401 ggtatcattc cgatcggcgc ggacggacgc atcctgatga tcgatcacta tcgcttcatt
     5461 accgatacac gcggctggga aattccgagc ggcggaatcg agcagggtga atccgtgttg
     5521 caaagtgcgc agcgtgaact tcttgaagaa gcaggttatt cggcgagtga ctggaaagtg
     5581 ttgggcaaat accacccctc gaacggatcg agcaatcagg ttttccatgc cgcgatcgcg
     5641 cggcagctca atcgggaatc tgaaccaacc gatgtgaatg aaactttggg attgcgctgg
     5701 ttcacgatcg atcaagtgcg gcagttgatc gatcgcaatg gaatccgcga cggtttgtca
     5761 ttgactacgt tgtgttgggg aatgatcgta ggactcacat gaagatactc gccattagcg
     5821 atcaagttga ggacgcgttg tacaaaccat ccatcgtcga tcgatttagc gatattgatc
     5881 tggtgatcag ctgcggcgat ctgccgtaca gttatctgga atatatcgtg acggtgctga
     5941 ataagccgat gtattacgtg catggcaatc atgatctcac gcttgaatat accgagagcg
     6001 gcgcgcagcg attgattccg gccggtgttg agtcgttgaa ttcgcgcacg acgatcggac
     6061 cgcagggcat gctgctggcg ggactggaag gatcgatccg atacgatccg cgcagcgcct
     6121 ttcaattcac gcaaagtgaa atgaatttga aggcctggcg attgtcgctg cgcttgatcc
     6181 gaaatcaatt gcgctatggc cgttatctcg atgtgctggt cacgcattcg ccgccgttcg
     6241 gcgtgggcga cggccccgat tatgcgcaca tcggtttcaa agccttcaat aggctgatta
     6301 aacgctatcg ccccaaactt ttattgcatg ggcatcaaca tacttacctg ggcctgaagc
     6361 cgggcacgtt agttgaagcg accaatgtgt tcaatgtttt tccgtatcgc gtgatcgaat
     6421 gggagtatgg cgatgagtga tgatggtcgc gctcaaaacg aatacgaacg catgcgccgc
     6481 cgcacacttt tccgcgatgt gctagcccgc ttggctggcc gccctaaccg gttgttatcc
     6541 tatgaagaag tgaaacaatc gttggagttg ggcggcccga tctatcgtgg tctgaagcct
     6601 gtgcctgtcg tacagatcgt cggcagcgtt gatcgctatc gcgacttcga tgcagaattt
     6661 atgcccgcgc aggatcgcac tgccgatcgt tggaagagcg tggcgcgcgc gtattacgat
     6721 gaagtcaatt tgccgcccgt gcgcttgtat aaagtcggcg atgcgtactt cgtgctggac
     6781 ggtcatcatc gcgtctcggt ggcgcgcgag cagggcgtgg cgttcatcga tgccgaagtg
     6841 ctggaagcgg tctcgcgtgt gccggtcagc gccgatctgc gcgccgaaga tttggaagtg
     6901 ctgcacgagt atcggcattt tctggagcgc acccggctgg atgagatccg gcccgatcag
     6961 cacattcgct tcacggtggc tggcggctac gatcgattga tcgagcacat ggcgatgcat
     7021 cgctacttca ttcaagtcga atggggtcgc gacgttggcg aagagcaagc cgtggcacat
     7081 tggtatgaca cggtctatat gccagtcgtc gaagcgatcc gcgcgcacaa tgtcttggcc
     7141 gatttcccgc atcgcaccga atctgatgtg tatttgtgga tcgtcgagca tttgtattat
     7201 ctgcaggaaa aagagaaaga tgtttcgatc gaagaagcag ctgaagaata tgccgatcaa
     7261 ttcagcgagc ggccaatcaa aaagatcgtg cgcagtgtga agcaggcttt tgaaggcact
     7321 gaaacgcgat cggactctga agcccaaaaa tcaaatggca tggaatcgga gaagaatgtc
     7381 acaacttaaa agatcgatcg ttaccgtgtt tggcaccaac gcgccgaaag cgggcgaacc
     7441 ggattacgag caagcgcgcg cgttaggtgc tgtgttggcg aaggcaggct atgtggtggc
     7501 gaccggcggt tacttcggca cgatggaagc cgcgagccgg ggcgcgaaag aagcgggcgg
     7561 acatgtgatc ggcgtgacat cttcagtttt tgatgactta cgactcactg caaatccgta
     7621 tttggatgaa gagatcaaat ttcccacttt gttccaacgc cttcatcatt tagtgacctt
     7681 cgccgatgcg tgggtcgcgc tgcccggcgg catcggcacg ttgagcgaag tcgcgttaac
     7741 gtggagtttg atgcaagtcg gcgagaagtc gcggcagccg tttgttctgg ttggctcgat
     7801 gtggcgcgac ttgctggaaa atttttcaaa gaacggatat gtgaaggaag agtatcatca
     7861 attgatcaga tatgcagatg atattgacgc ggtattgggg ttgttgaagt aatcgtattg
     7921 cgtcgtaacc ttatgcacaa cttttttgaa tggcaaaaga ttcaacgtca ttgcgaggcg
     7981 catctttttg cgccgaagca atctcagctg attgtcgtgg agatggcttc gcttcgctcg
     8041 caatgacggt tctcgtttac ttgattcaaa aagttgcgtg gtacgcaaga acgaagcatg
     8101 caacacgcac cacatttcat caactcaaat tattttgatg aggaacgatc atggctgaca
     8161 aacttactcc acgcagtgaa gattattcaa agtggtacaa cgatctggtg cagcgcgccg
     8221 aactggccga ttactcgccg gtgcgtggct cgatggtgat ccggccctat ggctacggct
     8281 tatgggagaa cgtgcaggcc gggctcgatc ggcgcttcaa ggcgaccggg catcagaatg
     8341 cctactttcc gctgctcatc ccgatgtcgt tcctgcaaaa agaaaaggaa cacgtcaaag
     8401 gctttgcgcc cgaactcgcg gttgtgacac atgccggtgg cgaaaaactg gaagagcctt
     8461 tggttattcg cccgacatcc gaaacgatca tcggccatat gtacgcgcag tggatcaaga
     8521 gttatcgcga tctgccgatc ttgatcaatt gctggaacaa cgtcgtacgt tgggaactgc
     8581 gcacgaaatt gtttttacgc acggcagaat ttttgtggca ggaaggccac accgcgcatg
     8641 ccaccgccga agaagctgaa gaggaaacca tccgcatgtt gaatgtgtac gccgacttcg
     8701 cagtgaacga cgcggcgatt ccggtgattc cggggcgcaa atcggattcg gaaaaattcg
     8761 ccggcgcagt tcgatcgtat acgatcgaag ccatgatggg cgacaagaaa gcgctgcagt
     8821 cgggcacgtc gcattttctc ggccaaaatt tcgcgaaggc atttgagatc aagtatctga
     8881 gcaagaacaa cgcacaagaa tttgtgtgga cgacctcgtg gggtttatcg acgcggatga
     8941 tcggcggcat catcatggtg cacggcgacg atcaaggttt gaagctgccg cctaaactcg
     9001 cgccgatcca agtagttatc gtccctatct tt
//
LOCUS       DASRTJ010000031         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_1681,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000031 DASRTJ010000000
VERSION     DASRTJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="glp"
                     /locus_tag="VFF70_00625"
     CDS             <1..1093
                     /gene="glp"
                     /locus_tag="VFF70_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZY43226.1"
                     /translation="MPLPSFPRSTMDGYAVRAADTFGASEALPAYLKVIGESPMGRAP
                     SIELNAGQTAIIHTGGMMPSGADAVVMIERTQKVTEAEIEVLRAVAPGENVLHVGEDV
                     KPNELLLPIGHVLRPQDLGGLMAMGVTSIKVARRPRVGILATGDEVIDPHTVPQAGQV
                     RDVNTYTLKGLIERSGCVAQSGGIIRDDFDHLVQAARSLIDQCDALVLSAGSSVSVRD
                     MTSDVINQLGQPGVLVHGISIKPGKPTILAVCNGKPVFGLPGNPVSALVVGDLFITPT
                     LWRMQGCDQPPTRQAVQARLAHNLSSVPGRVDYIQVKLIERAGETWAEPIFGKSNLIY
                     TLVKSDGMLIIPIDATGLQAGELVEVRLF"
     gene            1121..1912
                     /locus_tag="VFF70_00630"
     CDS             1121..1912
                     /locus_tag="VFF70_00630"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZY43227.1"
                     /translation="MAVIIAGHHRSGTSMFRLVCQSHPRVEINREMGNFLQIDTSWAD
                     YRRFMFQRWWNKRNASMRSDSTSKAPHPPTEMPRNLILVLRYLQRIRRHQHGTINAEI
                     IDRALHDLYPQAEVVGDKFPDYHFQLDRFASDKNLQCVFIYRDPRDVAASFVAKTRTV
                     WRAHTDDSVRQPAEIAERWVAAMHRMERTADRLFIVRYEDFVTNPQPIVKRLGEWLKI
                     DPALFNTRPIHAESIGKHRRGLSEQEICDVIEVAGTTMQLYGYTI"
     gene            1927..>2383
                     /locus_tag="VFF70_00635"
     CDS             1927..>2383
                     /locus_tag="VFF70_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HZY43228.1"
                     /translation="MAPNVYLEDIPLDEAWQCFTDALQTIGRWQSLESEELLIDRALG
                     RVTAEPIWARVSSPAYHSSAMDGYAVRAEDTVHATETSPLQLKIGSEAQYLDTGDPLP
                     AWANAVIQIEHVQPIGNDAIEIMSSIAPWTAVRSMGEDMVATELVLPANH"
BASE COUNT          541 a          621 c          697 g          524 t
ORIGIN      
        1 gatgcccctg ccttcatttc cgcgcagcac catggatggg tatgcggtgc gcgcggcaga
       61 taccttcggc gcgagtgaag cgctgcctgc ttacttgaaa gtgatcggtg aaagtccgat
      121 gggccgcgct ccctcgatcg aactaaatgc cggtcaaacg gcgatcattc ataccggcgg
      181 aatgatgcca agcggcgcgg acgccgtggt gatgatcgag cgcacgcaaa aagtaactga
      241 ggccgagatc gaagtcctgc gcgcggtggc gccgggcgaa aatgtattgc acgtcggcga
      301 ggatgtaaag ccgaatgagc tgctgctgcc gatcgggcat gtgctgcgcc cgcaagattt
      361 aggcggtttg atggcgatgg gcgtcacatc gatcaaagtg gcgcggcggc cgcgcgttgg
      421 gattttggca acgggcgatg aggtcatcga tccgcatacg gtgccgcagg cagggcaagt
      481 gcgtgatgtc aatacgtata cgctgaaagg cttgatcgaa cgatcgggct gcgtggctca
      541 atcgggcggc atcattcgtg acgatttcga tcatctggtt caagccgcgc gcagtttgat
      601 cgatcaatgc gatgcactag ttttatcggc gggcagttcg gtgagtgtgc gcgatatgac
      661 ttccgacgtg atcaatcaat tgggacagcc cggggtgttg gtgcacggta tatcgatcaa
      721 acccggcaag cccacaatac tggcagtgtg caatggcaag ccggtgtttg gcctgcccgg
      781 caatccggtt agcgcgttgg tcgtcggcga tctgttcatc acgccgacgt tgtggcgaat
      841 gcaaggctgc gatcaaccgc ctacgcgcca agcggtgcaa gcgcgactgg cgcacaacct
      901 gtcttcagtg ccgggccgcg tcgattacat tcaagtgaag ttgatcgaac gcgctggcga
      961 aacgtgggcc gaaccgatct ttggcaaaag caatttgatc tacacgctgg tcaagtccga
     1021 tgggatgctg atcattccga tcgatgcaac cggacttcaa gccggtgaat tagttgaagt
     1081 gagattattt tgactgcaaa acttttttag caggcgaagc gtggcagtaa tcattgcagg
     1141 gcatcaccgc tcaggaactt ctatgtttag actcgtgtgc cagagtcatc cgcgggttga
     1201 aatcaatcgc gagatgggca acttcttaca aattgatact tcatgggccg actatcgacg
     1261 gttcatgttt cagcgctggt ggaacaaacg gaatgcatcc atgcggtcgg attcgacttc
     1321 caaggcgccg catccgccca ctgagatgcc gcgcaacctg atattagtac taagatattt
     1381 acagcgcatt cgccgccatc aacacggcac aattaatgcg gaaatcatcg accgcgcatt
     1441 gcatgatctt tatccgcaag ccgaagttgt gggggataaa tttccagact atcactttca
     1501 acttgatcga ttcgcatcgg ataagaatct gcaatgcgtc tttatctatc gcgatccgcg
     1561 cgatgtagcc gcttcattcg tggctaaaac ccggaccgtc tggcgtgccc acaccgacga
     1621 ttccgtccgc caacctgccg aaattgccga gcgctgggta gcggcgatgc atagaatgga
     1681 acgcactgcc gatcgattgt tcatcgtccg ctacgaagat ttcgtaacga acccgcagcc
     1741 tatcgtcaaa cggctaggag aatggctgaa gatcgatccg gctctcttta acacgcggcc
     1801 gatccatgct gaaagcatcg gcaaacatcg gcggggtttg agcgagcagg aaatatgcga
     1861 tgttattgaa gtcgctggga ccacgatgca gctttatggc tataccattt agatttgaga
     1921 atttcaatgg cgcccaacgt ttacctcgaa gacattccac tggatgaagc ctggcaatgc
     1981 tttactgacg cgctgcaaac gatcgggcgt tggcaatcgc tcgagagtga agagctgctg
     2041 atcgatcgcg cgttgggccg cgtgaccgcc gagccaatct gggcgcgcgt ttcatcgccc
     2101 gcgtatcatt cgagcgcgat ggacggctac gccgttcgcg ccgaagacac ggtgcatgca
     2161 actgaaacaa gtccgttgca actaaagatc ggatctgagg cccaatatct ggacacgggc
     2221 gatcctctgc ccgcctgggc gaatgcagtg atccagattg agcacgttca acctattggg
     2281 aacgatgcga tcgaaatcat gtcgtcgatc gcaccgtgga ctgcggttcg atcgatgggc
     2341 gaggatatgg tcgcgactga attggtcttg ccagccaatc acg
//
LOCUS       DASRTJ010000032         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_77273,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000032 DASRTJ010000000
VERSION     DASRTJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77273"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFF70_00640"
     CDS             complement(<1..491)
                     /locus_tag="VFF70_00640"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZY43229.1"
                     /translation="MASPTSNLQPPTSIAILNIPVHDVTMSETLDWINRFIVERSSHQ
                     ICTINPEFIMAAQKDSEFKRILNHSALNIPDGVGILWAARRLGHRLRERVGGSDLVLQ
                     IAQRAAIAGWKIFMLGAAEGVAEQAAGQLRSNFPAINIVGAFAGSPSIDREAELADRI
                     RSTS"
     gene            complement(493..1620)
                     /locus_tag="VFF70_00645"
     CDS             complement(493..1620)
                     /locus_tag="VFF70_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY43230.1"
                     /translation="MINRKPSSSAALIGIDASRSIGDRPTGTELYSRYLIETLIDQTH
                     PDYSFRLYYNQPPKSLSPNPRSAIRIIPFPRLWTHVRLSLEMLLHAPDLLFIPAHVIP
                     IIHPRRSIVTVHDLGYLHFPEAHPPRQRWYLDRSTRWNVRSASRAIADSEATKRDLID
                     RYHAEADRISVAYPGIDPSIQRVADPIEIDRVKAKYNIDDDYILYLGTLQPRKNLSRM
                     ITAFGQVQSNLQLVLAGKTGWYADPLFKQVADHSLSARVLFPGYTAEADKSALLSGAT
                     ALVFPSLYEGFGFPVLEAMRCGVPVLCSNTSSLPEVSGDAALLIDPLSIESIADGLTR
                     IINDQALRQKLIAAGYAQAKKFSWRSCADDVLKVIQQVLNS"
     gene            complement(1601..2179)
                     /locus_tag="VFF70_00650"
     CDS             complement(1601..2179)
                     /locus_tag="VFF70_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43231.1"
                     /translation="MDRRGALIPALVLILIGVFFLLVNLGVLPKLSITELWPGIVMFV
                     GVMFWLGFIFGREHDAGLAFAGTIITLIGAFFFLFTLHTFVPGYGFIQWGDQGRLWPA
                     YPAIVGIAFVVLWIANRFRDAGVLIPAAILLAVGLAGFSFTLGDVTPFRDIVRFWPVL
                     LIGLGLVILVRAFVRPNRSVTPPTGMNDQPKA"
     gene            complement(2235..2849)
                     /locus_tag="VFF70_00655"
     CDS             complement(2235..2849)
                     /locus_tag="VFF70_00655"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZY43232.1"
                     /translation="MKTEILSPDQLDLAAQLLRDHQLVAFPTDTVYGLGTLAFDGATV
                     AKLYAAKDRSIEKAIPILIGDIDQLEQIAIEIPLIAHKLIEQFWPGALTIVLKKHPNI
                     PIEVSATETIGVRMPDLDLTRNLIRLTGPLAVTSANRSAGLNPRTALEVLAQLNDRID
                     AVIDGGSTPGNVSSTVIDCTQSLPTILREGALTSAVMNFLRETL"
BASE COUNT          716 a          741 c          842 g          636 t
ORIGIN      
        1 gaagtcgatc gaatccgatc cgcaagctcg gcttctcgat cgatcgacgg cgatccggcg
       61 aacgcaccga cgatgttgat cgctggaaag ttcgatcgaa gttgacccgc cgcttgttca
      121 gcaacaccct cggctgcgcc cagcataaat attttccaac cggcgatcgc tgcgcgctgt
      181 gcgatctgca gcaccaaatc cgatccgccc acgcgctcac gcagacgatg acccaatcga
      241 cgcgcggccc ataatattcc gacgccatca ggaatattta gcgcagaatg attgagtatc
      301 cgcttgaact ccgaatcttt ttgcgcggcc atgatgaatt cgggattgat ggtgcagatt
      361 tggtgtgatg atcgctcaac aatgaaacga ttgatccagt cgagtgtctc gctcatcgtc
      421 acgtcgtgga cagggatatt gaggatggcg atggaagttg gaggttggag gtttgaagtt
      481 ggagaggcca tgtcaactat tcaagacttg ttgaattact ttcaggacgt catcggcgca
      541 agatcgccac gagaattttt tcgcttgtgc gtaaccggca gcgatcaatt tttgtctcaa
      601 ggcttgatcg ttgatgattc tcgttaaacc atctgcaatc gattcaatgc tcaacggatc
      661 gatcagcagc gccgcatcgc ctgagacttc cggcagcgat gaggtattgc tgcacaacac
      721 aggcacaccg catcgcatcg cttcgaggac gggaaagccg aagccttcat agagagaagg
      781 aaacactagc gcggttgcgc cgctgagcaa cgcggattta tcagcctcag cagtatagcc
      841 tggaaacagt acacgcgctg agagagaatg atcggccact tgtttgaaga gcggatcggc
      901 ataccaaccg gttttgcccg cgaggactaa ctggaggttg gattggactt gcccaaacgc
      961 cgtgatcatt ctcgacaagt tttttctggg ctgcagtgtg ccgagataca aaatatagtc
     1021 gtcgtcgata ttgtatttcg ctttgacgcg atcgatctca attggatcag ccacacgctg
     1081 gattgaagga tcgatgcccg gataggctac gctgatccga tcggcttcag cgtgataccg
     1141 atcgatcaga tcgcgctttg tcgcttcgga atcggcgatg gcgcgtgatg cagatcgaac
     1201 attccagcgc gtcgatcgat ccagatacca gcgttgtcgc ggcggatgcg cttcgggaaa
     1261 atgcaagtaa ccgagatcgt gaacggttac gatcgatcgg cgcggatgaa tgatcgggat
     1321 cacgtgcgct ggaatgaata agagatcggg cgcatgtagc agcatctcga gcgacaatcg
     1381 aacgtgcgtc cacaatcgcg gaaagggaat aattcggatt gcggatcgcg gattggggga
     1441 taaagatttg ggcggttggt tgtaatagag gcgaaatgaa taatcaggat gagtttgatc
     1501 gatcagggtt tcgatcaagt aacgcgaata caattccgtg cccgtcggtc gatcgccaat
     1561 cgatcggctg gcatcaattc cgataagcgc agcagatgaa ctaggctttc ggttgatcat
     1621 tcatcccagt gggcggtgtg actgatcgat tcggccgcac gaacgctcga acgagaatga
     1681 cgagacccag tccaatcaag agcaccggcc agaaacgcac aatgtcacgg aacggagtga
     1741 cgtcgcctag cgtgaaactg aagcccgcca gtccgactgc aagcagaatc gccgccggga
     1801 tcagcacgcc cgcatcgcgg aatcgattcg cgatccacaa cacgacgaag gcgatgccga
     1861 cgatcgcggg ataggccggc cacaagcggc cttgatcgcc ccattgaatg aagccgtagc
     1921 cgggcacaaa agtatggagc gtgaagagaa agaagaacgc gccgatcagg gtgatgatcg
     1981 tgccggcgaa ggcgagaccc gcatcatgct cacggccgaa gatgaatccc agccagaaca
     2041 tgacgccgac aaacatgacg atgcctggcc acaattcggt gatggagagt ttgggcagca
     2101 caccgaggtt gaccagcaga aagaatacgc cgatcaaaat taaaactaaa gccggaatca
     2161 atgcaccgcg tcgatccata ttgcacctcc gagtgaatta ccccgctgct atgtcgacga
     2221 cgttttcgat tgtatcacaa agtttcgcgc aagaaattca tgaccgcact ggtcagcgcg
     2281 ccttcgcgca atatggtcgg caatgattgc gtgcaatcga tcacagtgga ggagacattg
     2341 cccggcgtcg atccgccgtc gataaccgcg tcgatgcgat cgtttaattg cgccaacact
     2401 tctagcgctg tacgcggatt cagccctgcc gatcgattcg cactggtgac agccagcggg
     2461 ccagtgaggc ggatcaagtt gcgcgtgaga tcgagatcgg gcattctgac gccgatcgtt
     2521 tcagtagcgc tgacttcgat cgggatgttt ggatgcttct tcaagacgat agttaatgcg
     2581 cccggccaaa attgttcgat cagcttatgc gcaattaatg gaatctcgat cgcgatctgt
     2641 tcgagttgat cgatgtcacc gatcaagatc ggtatcgctt tttcgatcga tcgatcttta
     2701 gccgcgtaga gtttcgctac cgttgcgcca tcgaaagcga gggtgccgag gccgtagacg
     2761 gtgtcagtcg gaaacgcgac gagttggtga tcgcgcaaca attgtgcggc gagatcgagt
     2821 tggtcagggg agaggatctc ggtcttcatt tcagttgggg cggacggccg tccgccctta
     2881 cattttaact cggaggatct cgatgttggt cacgttgtcg cggtcgaacg taagg
//
LOCUS       DASRTJ010000033         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_77305,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000033 DASRTJ010000000
VERSION     DASRTJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77305"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="VFF70_00660"
     CDS             <1..>1595
                     /locus_tag="VFF70_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HZY43233.1"
                     /translation="VLALGLVLVVIGHTAAPSVSLPARQSQTVASAAKILVGQAGLIE
                     VSAADLISSDLGWKSIDPTSVRLFFQDRQQPVWLEGQGAALTLKFYGQAADSRYTREN
                     AYFFQIDQSLVPPLPMLTQTLVERSSGAIDHYTATLHAEENFLYSPLVANGDHWFWAQ
                     LPAPQSKMFTVELSAVVDGPAQLTIGVWGSTEASASPDHHLRIKINRQNVADQTWDGI
                     ARNTLKADLPRGLLVAGSNIIQIEAPGDTGVAADITFLDWIEIRYLRHFVAENDRLEF
                     DGVDGAVQLSGFSGPIDLFDITVPDRLVRLIGPSTLTQGSIEIGVQADHHYMAVGPHG
                     YQSAVRIVPAATSPDLKAADNGADYIAIGPADLLAPLQPLLNWHQTQNLKVVAIPAET
                     IYDQFGNGLPEPEAIHSFVQYAVQHWTPAPKFVLLVGDATYDPLGYSGLPDGNRLPTF
                     LVQTVYGGETASDVGFVQLNDDPWPDMPIGRVPARTAEQVTTWVNKTLAYEQSAPVGD
                     WRQRILAVADGQESSFQSEAAAF"
BASE COUNT          335 a          438 c          443 g          379 t
ORIGIN      
        1 gtgctggcac tcggcctggt gctcgtggtg atcggtcaca ctgctgcgcc atctgtgtca
       61 ctccccgcac gtcaatctca aacggtagca tcggcagcga aaatcttagt tggtcaggct
      121 ggcctgatcg aagtatctgc cgccgatctc atctcgagtg atctaggctg gaaatcgatc
      181 gatccgacaa gtgtccgatt gttctttcaa gaccgacagc agcccgtgtg gctcgaaggg
      241 cagggcgctg cgctgacctt gaagttctat gggcaagcag ccgatagtcg atatacgcgc
      301 gagaacgcgt attttttcca gatcgaccag tcgcttgttc cgcctctacc gatgctgacg
      361 cagactttgg ttgagcgatc gtcaggcgcg attgatcatt acaccgccac gctgcatgcc
      421 gaagaaaatt ttctgtattc accgctcgtc gcgaatggtg atcactggtt ttgggcacag
      481 ctgccggccc cgcaaagcaa aatgttcaca gtcgaattaa gtgctgtagt ggatggtcct
      541 gcacagctta ccatcggcgt atggggcagc acggaagcgt ctgcttcacc ggatcatcat
      601 ttgcgtataa aaataaatag gcaaaatgtg gccgatcaga cgtgggacgg tatcgcgcgc
      661 aacactctta aagccgatct gcctcgcggc ctgcttgtcg ctgggagcaa tatcatccag
      721 atcgaagcgc caggcgacac tggcgtagca gcggacatca ccttcctaga ctggatcgag
      781 attcgttatc tccgtcattt tgtcgctgag aacgatcgat tggagtttga cggtgtcgat
      841 ggggccgtcc agcttagcgg cttttcaggt ccgatcgatc tcttcgatat caccgtgccc
      901 gatcggctgg tgcggttgat cgggccgtcc acgctgactc agggatcgat cgagattggt
      961 gtgcaagccg atcaccatta tatggccgtc gggccgcatg gttatcaatc agccgtaaga
     1021 attgttccgg ccgccacgtc gcccgatctg aaagccgctg acaacggcgc cgactacatc
     1081 gcgattggcc ccgccgatct gttagcgcca ttacagccgc tattgaactg gcatcaaacg
     1141 caaaatctta aggtggtggc tatcccggct gagactattt acgatcaatt tgggaacggg
     1201 ttgcctgaac ccgaggcaat acattcgttc gtgcaatacg cggttcaaca ttggactccc
     1261 gccccgaaat ttgtattact cgtcggcgat gctacgtacg atccgttagg ctactccggc
     1321 ctgccggatg gcaatcgtct gccgacgttt ttggtgcaga ctgtttatgg tggtgaaact
     1381 gcaagtgacg tcggttttgt tcaattgaat gatgatccct ggcccgatat gccgatcggg
     1441 cgtgtaccgg cccgcaccgc tgagcaagtg accacctggg tcaacaagac gctggcttac
     1501 gaacaatcag cgccggtcgg cgattggcgt caacgcatcc tggccgtcgc cgatggacaa
     1561 gagtcatctt tccaatctga ggcggcagcc ttctt
//
LOCUS       DASRTJ010000034         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_304477,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000034 DASRTJ010000000
VERSION     DASRTJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304477"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1059)
                     /locus_tag="VFF70_00665"
     CDS             complement(10..1059)
                     /locus_tag="VFF70_00665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY43234.1"
                     /translation="MLKPGAVLRARYKVVESIGQGGMGAVYQAEDLRLEGRRCAIKEV
                     RLDLELDEEAGRQAQEQFYREASVLARLDHSTLPKVSDYFSINDTDYLVMDFVPGRDL
                     KDIMDEARRSNLFLNEADVLNWAAQLCDALSFLHRQEPPIVHRDIKPSNIKLTPDGYI
                     KLVDFGLVKVLLANDDNRTITVMQGRGTAAYTPLEQYGGDAGHTDVRSDIYSLGATLY
                     HLLTHQQPLDAKQRFLNPSALQSARAINPKISAQTDRAIMSAIALHPDLRPASIDEFH
                     DLMLASPGVTPAPINPRVSTNHTNHSWSQAFQSNRMLIAALIGLIVLAILITLIAAPS
                     ATAIHSSATPTALLR"
     gene            1212..1865
                     /locus_tag="VFF70_00670"
     CDS             1212..1865
                     /locus_tag="VFF70_00670"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY43235.1"
                     /translation="MQDSMIPILVVKEGQLAGKRWPLDKPALTIGRGEDCDIVLPDRQ
                     VSRQHIRVLHDVQGYAVEDLRSKNGTYLNGVPVQGIARLQDGDEIQIALSVRLLFVGA
                     EATLPLTSEMVKISVPGVRLNKSQRQVWVGGNAIDPPLSISQYRLLELLWERKGKVVT
                     REEVIAAVWPESDEAGITEQAIDALMRRLRDRVAELDPDHEYLVTVRGHGFRLDDRP"
BASE COUNT          443 a          493 c          563 g          409 t
ORIGIN      
        1 cccttacatt taacgcagta aggctgtcgg tgtcgccgag gaatgaatgg cggtggccga
       61 cggtgcggca atgagagtaa tcagaatcgc taaaacaatc aacccgatca gcgcggcgat
      121 caacatgcga ttagactgga atgcctgcga ccatgagtga ttagtgtgat tcgtgctaac
      181 gcgcggattg atcggtgcgg gcgtcacgcc gggcgaggcc agcatcagat cgtggaattc
      241 atcgatcgaa gccgggcgca gatcgggatg cagcgcaatc gcggacatga tcgctcgatc
      301 ggtttgggct gaaattttcg gattgatcgc ccgcgccgat tgcagcgcgc ttggatttaa
      361 gaaacgctgt ttggcatcca gcggctgttg atgggtcagc agatgataca atgttgcgcc
      421 tagcgaataa atatcgctgc gcacatccgt gtggcccgcg tcgccgccat attgttcgag
      481 cggtgtgtaa gccgcggtcc cgcggccttg cataacggtg atggtgcgat tgtcgtcatt
      541 cgccagcagc actttgacca gaccgaaatc gacgagctta atatacccgt cgggtgtgag
      601 cttgatgtta gagggtttga tgtcgcggtg cacgatcggc ggttcctggc gatgaagaaa
      661 ggacaacgca tcacacaact gcgctgccca attcaagaca tcggcctcgt tcaggaacaa
      721 attcgatcgg cgcgcttcat ccatgatgtc tttgagatcg cggccgggca caaaatccat
      781 gaccagataa tcggtatcgt tgatggaaaa gtaatcggag actttgggca gcgtggaatg
      841 atcgagacgc gcgaggacgc tggcttcgcg gtaaaattgt tcctgcgctt ggcggccagc
      901 ttcttcatcc agttccagat cgagccggac ttctttgatc gcgcagcggc ggccttccag
      961 acgcaggtct tcagcttgat agaccgcccc catgccaccc tgcccgatcg attcaacgac
     1021 tttataccgt gcgcgcagca ccgcgccggg ctttaacatg atactcctct tctcctgaca
     1081 gaaagcttaa ctgacatcga aggcattgtc aacctcacct aaccccaacc cctgccctcg
     1141 tccgatgcta gttgaaattt tatatcggga gaggggacag gcaaggtgaa gggcgaggga
     1201 tggaggcaaa tatgcaagac agcatgattc caatcttagt tgtgaaagaa gggcagttag
     1261 ccggaaagcg ctggccgctc gacaagcccg cgctgacaat tggccgcggt gaggattgtg
     1321 acatcgtgct accggatcga caggtgagcc gccagcatat ccgcgtgctg cacgacgttc
     1381 aaggttacgc agtcgaagac ctgcgtagca aaaacggaac ttatttgaat ggcgtgcccg
     1441 tgcagggcat cgcccgttta caagatggtg atgaaatcca aatcgcgttg agtgtgcgcc
     1501 tgctctttgt cggcgcagaa gcaaccctgc cgctcacctc cgagatggtt aaaattagtg
     1561 tgcccggcgt gcgcttgaac aaaagtcagc ggcaagtgtg ggtcggcggt aacgcgatcg
     1621 atccaccgtt gtccatctcg caatatcgct tgctggaatt attgtgggaa cggaaaggca
     1681 aagttgttac acgcgaagaa gtcatcgccg cagtgtggcc cgaatcggac gaagcgggca
     1741 tcacggaaca ggcgatcgac gcactgatgc gccgcctgcg tgatcgcgtg gccgaactcg
     1801 atccagatca cgagtatctg gtgacggtca gaggacacgg ctttcggtta gatgatcggc
     1861 cataaccatg acgaatgcag gtatgatttg tgactgacct attgacga
//
LOCUS       DASRTJ010000035         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_39608,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000035 DASRTJ010000000
VERSION     DASRTJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39608"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..409
                     /gene="sucC"
                     /locus_tag="VFF70_00675"
                     /pseudo
     CDS             1..409
                     /gene="sucC"
                     /locus_tag="VFF70_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
     gene            653..1522
                     /gene="sucD"
                     /locus_tag="VFF70_00680"
     CDS             653..1522
                     /gene="sucD"
                     /locus_tag="VFF70_00680"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZY43236.1"
                     /translation="MSILVGKHTRLLVQGITGREGEFHTKQMIEYGTQVVGGTTPGKG
                     GEWAAGVPVFDSVKEAVDATDANVSIIYVPARFAPDAIYEAADSGLKLIVCITEGVPV
                     LDMIRVRAYLDQKKVRLIGPNCPGLITPGEAKVGIIPGHICKPGGIGIVARSGTLTYE
                     VIYALSNRGMGQSTCVGIGGDPINGTNFIDVLQMFEDDPLTDQVVLIGEIGGSDEERA
                     ADFIAARMSKPVTAFIAGRTAPAGKRMGHAGAIIEGGAGTAAGKIAAFEAVGVRVAQH
                     PEEIPDLVAYAAR"
     gene            1582..1989
                     /locus_tag="VFF70_00685"
     CDS             1582..1989
                     /locus_tag="VFF70_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY43237.1"
                     /translation="MAGIPCVAVIIKNSHDQVLLLLRDNKLGLSFANHWTLPGGRVEF
                     NETIEAAAQREVQEEIVLELPLLAWKTYERITRDVLIEQHVFVSASDHSLVEMTLGEG
                     QALRFFGEEDIPILPIGFEFEKLLAEFFREKPI"
     gene            1973..>2873
                     /locus_tag="VFF70_00690"
     CDS             1973..>2873
                     /locus_tag="VFF70_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HZY43238.1"
                     /translation="MKSRFSLRIDLHLTTVILIAVIAALPFITQSGLPRHTDLELHVF
                     RAAEYSQVLSGGVLYPRWAPDFYYGYGYPIFNYYAPFTYALASLFAIPFGIVAGVKGA
                     ILLAFFLAAIGMYYFARRHFGSIGGVIGSAAFVLSPYILFIDPLMRGDLAEFFALSLI
                     PWLFLVFDRPAQPLGRTQAYRALVLGILIHSHNLTALFALGILAAYLLWRGLTIDTPR
                     RWVADFGAVVFALLLTSIFWLPFIAERSAIRLDVAGPGHFDYHNHFIQLSMLLSPSPA
                     IDLGATTPKFIYNLGLVQWLLIIP"
BASE COUNT          632 a          699 c          843 g          699 t
ORIGIN      
        1 gtcgtatatt aaactggatg gcgagatcgg gtgcatggtg aatggcgcgg gcctggcgat
       61 ggcaacgatg gatatgacgc agttgttcgg cggcagcccg gccaacttcc tcgatattgg
      121 cggcggcgcg caagccgata aggtagcggc ggctctccga ttgatcttgg ctgaccctaa
      181 agtgaaagct gtgttgttta atattttcgg cggtatcacc cgctgtgacg aagtggcgcg
      241 cggcatcgtc gcggccttgg aagaagtgaa accgcatgtg cccatggtgg cacgcttagt
      301 aggcacgaat gaagaagagg gacgccagat tctggctgcg gccaaaatgc aaacagccaa
      361 gactttggct gaagccgccc aaaaggccgt cgctgcggtg aaaggtgaag cggccgtcgt
      421 gaagttgccg cccgtttcgc tggagccgct caatgtcgcg cgaaagactg tcaggccgcg
      481 taaaccaatc ggaaaaaaga aacaaccgaa aaagaaattg atcgcacgtt caaaatcaac
      541 accgaagacg tcggcgagaa aatcaatcaa gaagaaatca aaatcgagag catgaaacca
      601 tgatcaaaac tatctgcgat aggcactatg ctatccgcgg tgtggaggct gaatgagcat
      661 cctggttggc aaacacacgc gcttgttggt gcagggtatt accgggcgcg aaggcgaatt
      721 tcacacgaag caaatgattg agtatggcac gcaggttgtg ggcggcacaa cgccgggcaa
      781 aggcggcgag tgggccgcag gtgtgccggt cttcgattcg gtcaaagaag ccgtcgatgc
      841 gaccgacgcg aatgtatcga tcatttatgt gccggcgcgc tttgcgcctg atgcgattta
      901 tgaagcggcc gattcgggac tgaagttgat cgtgtgcatc actgaaggcg tgccagtctt
      961 ggatatgatt cgcgtgcgcg cgtatctcga tcaaaagaaa gtacgcttga ttggcccgaa
     1021 ctgtccgggt ttgatcacgc cgggtgaagc aaaagtggga attattcccg gccatatttg
     1081 caagccgggt ggcatcggta ttgtcgcgcg cagtggcacg ttgacttacg aagtgattta
     1141 cgcgttgagt aatcgcggca tgggacagag cacgtgcgtg ggtatcggcg gcgatccgat
     1201 caatggcacg aatttcatcg atgtgctgca gatgtttgaa gatgatccgt tgaccgatca
     1261 agtggtactg attggcgaga tcggcggatc ggacgaagaa cgcgcggcag atttcatcgc
     1321 agcgcgcatg tccaaaccgg tgacggcctt catcgctgga cgaactgcgc cagcaggcaa
     1381 acgcatggga catgccggcg cgatcattga aggcggcgca ggcacggcgg cgggcaagat
     1441 cgctgcgttt gaagcggtag gcgtaagagt cgcgcagcat cccgaagaaa ttccagactt
     1501 ggtcgcttac gcggcacgat aaatgcagcc agcataaaaa gagccgactc attgtcggct
     1561 ctagttttat attggagcag tgtggctggt attccgtgtg ttgcggtcat tatcaagaat
     1621 tcacacgatc aggttctgct cttgttgcgt gacaacaaac ttggtctgtc ttttgccaat
     1681 cactggacgt tgcccggcgg acgcgtcgag ttcaatgaga ccatcgaagc agccgcacaa
     1741 cgcgaagtgc aagaggaaat tgtgttagaa ttacccctcc ttgcttggaa gacttacgaa
     1801 cgaattacgc gggacgtttt aattgaacag catgtatttg tgagtgcaag cgatcacagc
     1861 ctagttgaga tgactctggg tgaagggcag gcgctccgtt tcttcggtga agaagatatc
     1921 ccaatcttac cgatcgggtt tgagtttgaa aaattgctag cagagttttt tcgtgaaaag
     1981 ccgatttagc cttcgtatcg atcttcatct cactacagtt atcttgattg cagtgatcgc
     2041 ggcactgccg ttcattactc aatcgggttt gccgcgtcac acggatcttg aactgcatgt
     2101 ctttcgcgcc gccgaataca gccaggtgct gagcggcggc gttctctatc cacgttgggc
     2161 gccggatttt tattacggtt atggctatcc gatcttcaac tactatgcgc cgttcactta
     2221 tgcattagcc agtttatttg caatcccgtt tggcatcgtg gcgggtgtga agggtgcgat
     2281 cctgctcgcg tttttcttgg ccgcgatcgg aatgtattat tttgcgcggc ggcattttgg
     2341 atcgatcggc ggcgtgatcg gatcggcagc gtttgtgttg tcgccgtaca tcctgtttat
     2401 cgatccattg atgcgcggcg atctggccga gttctttgcg ttgagtttga tcccgtggtt
     2461 gtttctagtg ttcgatcggc cagctcagcc ccttggccgc acacaagctt atcgcgcgct
     2521 ggttctgggg atattgatcc atagtcacaa cctaacagcg ttattcgcgc tcggcatctt
     2581 agctgcatat ctgttgtggc gcggactgac gatcgatact ccgcgtcgct gggttgccga
     2641 ttttggcgca gttgtgtttg cgctgctgct gacctcgatc ttttggctgc ccttcattgc
     2701 tgaacgaagt gccattcgat tggatgtcgc cggtcccggc catttcgatt atcacaacca
     2761 cttcatccaa ctatcaatgc tactgtcgcc ctcaccggcg atcgatctcg gtgcgacgac
     2821 gccgaagttc atttacaatc tcggcctggt gcagtggctg ttgatcatac ccg
//
LOCUS       DASRTJ010000036         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_1889,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000036 DASRTJ010000000
VERSION     DASRTJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..854)
                     /locus_tag="VFF70_00695"
     CDS             complement(498..854)
                     /locus_tag="VFF70_00695"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZY43239.1"
                     /translation="MAALLQMKCIACRADSPPATTDEIHEWLLLLPEWKIIERDGVRQ
                     LARTYQLENFAKALALTDRVGEISENEGHHPDILTQWGKVTIWWSTHKIKNLHRNDFI
                     MAAKTDDIAQEESFAS"
     gene            1173..>3995
                     /locus_tag="VFF70_00700"
     CDS             1173..>3995
                     /locus_tag="VFF70_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZY43240.1"
                     /translation="MPRLSSSIEDIKDHYTVVVIGSGYGGGIAASRSARAGQQVCVLE
                     RGKEFQPGEYPDTELEALPEIQVNLPDQTLGSRTGLYDVHVSDDMNVLIGCGLGGTSL
                     INANVASRPERRVLDDPRWPQAFRDDIDTLLMEGYAHAEEMLGSNPYPDNLPPTRKML
                     AHQKSASAFSPAKFYRLPINVTFQDGVNKVGVQQHQCALCGDCMSGCNYGAKNTVLMN
                     YLPDAKNHGAEIFTQVSVRYLERKGNRWHVHYEILDVGQEGFEAPTSVVTADIVVLAA
                     GTLGTNEILLRSKAAGLSLSDQLGNRFSGNGDVLGFGYNNDVEINMVGFGSRKPGQFD
                     PVGPTIITAIDLREQPNLEDGMIIEEGGACGPLAAFMPKTLSLAAKLVGQDTDSGLAD
                     LIEENSRELDSFIRGAYHGAVKNTQVYLVMAHDDSGGNLTLENDRLQIKWPGAGDKPY
                     IESVNNSLVRATKPLGGTYTENPAWSKLQAKNMVSAHPLGGCVMAEDAEQGVTNHKGQ
                     VFSGPSGSQVYAGLYVNDGAIIPRSLGTNPHITISAVAERNMLLLAKDRGWTIDYSFG
                     QVAQPIELGVFPAKPGIQFTEAMEGWFSTKIKDDFEAGLRQGQADGSSFRIIVTIRSD
                     DVERMMSDPDHAAQLVGTATAPTLSPQPLTLTDGKFYLFIQDPNQFNVRFMRYRMKMT
                     AQDGQVYTFDGNKVIHNEPGFDMWADTTTLFITVYSGPDMNGPVIGKGVLKILLKDLR
                     HQLTTMQVPNAKDLGQRISILARFGRFFCDQLFDLYGGVFARPSVFDPGAAPRKKRPL
                     RAGVPEVYFFKTEDNLQLRLTRYQGGSKGPVILAHGGAVSSLIYSTDLIETNLTEYLY
                     AHGYDVWLLDYRASIELPYATALYTGDDIALYDWPAAVQKVLERTGASSVQAVTHCYG
                     AITFNMAMQAGLQGVRSAVCSQIGSHP"
BASE COUNT          980 a         1090 c         1053 g          872 t
ORIGIN      
        1 aggtataagc cttgcagatg aatattagat gagaagtgaa tgaaaagaca cgacgctgat
       61 cctacatatg cgctacgtgc aaacaacgct cacggcctta tagcacataa gcgatcaatt
      121 tgtctttacg tgttacgcac gaataatact gagataacgc agccgtgaaa aattcgttgc
      181 aaatatattg aatcgattga cacactagat tagacgttta taaggcgagg agtgttccat
      241 cgatcactcc aatgtcgatc aatttgatcg atctgaaatg ctgaatcgat gaatgtccgg
      301 aagacgcctg aatccgatcg gcgatcaatt ggaaatctca tccaccctgt caccacacca
      361 aggaaaaagg caataacagt ttgtctcagt ttatcgcatg gagatgagct aacgcgtgca
      421 ctatttggca ccgccgtgcg aatatccgat tacgcaatgg catgatagtg tactagcgcg
      481 caggcacaat ctgccgatca ggatgcaaat gattcttcct gcgcaatgtc atcggtcttc
      541 gcggccatga tgaaatcatt ccgatgcaga ttttttatct tgtgcgttga ccaccagata
      601 gtaaccttcc cccattgcgt caatatgtct ggatgatgac cttcattctc cgagatctcg
      661 ccaacacggt cagtcaatgc caacgctttt gcaaaattct caagctgata ggtacgtgcc
      721 aattgccgca cgccatcacg ttcgataatc ttccattctg gcaatagcag cagccattca
      781 tgaatttcgt cagttgtcgc cggcggcgaa tcggcacggc aagcgataca tttcatttgt
      841 aagagcgcag ccatgttatc ccccttagtg taagtaacaa tcgcacaagc tatgagccgg
      901 tttcatgcgg aggcttgtga cttagccact cgctgacctt gcgtacaatg gttgattttc
      961 cccaattgag tggacggggt gttacaccat cgtcaccgcg tgttcgaggt aatccttgca
     1021 gcgagccaag cggttccgtt taatttagac ctgccgagat ttgctaattg cagagttcgg
     1081 atatacactt tactttattc tgtatcgtgt gtcaaagcat ggatgaagtc ggattcctag
     1141 ccagaaccga tatagcccaa gagaggaaaa acatgccccg tttatcatcg tcgatcgagg
     1201 acatcaaaga tcattacaca gtcgtcgtga tcggatcggg ttacggcggc ggcattgccg
     1261 cgtcacgttc ggcgcgcgcc ggtcaacaag tctgcgtgct tgaacgtggc aaggagtttc
     1321 agcctggcga atatccggac accgaactcg aagcgctccc tgaaattcaa gtgaacttgc
     1381 ccgatcaaac gcttggctcg cgcaccggcc tctatgacgt tcacgtcagc gacgacatga
     1441 atgtgctgat cggctgcgga ctgggcggca cgtcgttgat caatgccaac gttgcctcgc
     1501 ggcccgaacg gcgcgtgctc gacgatccgc gctggcccca agcatttcgc gacgacatcg
     1561 atacactgct gatggaaggt tatgcgcacg ccgaagagat gttaggctcc aatccgtatc
     1621 ccgataatct tccgccgacg cggaagatgc tggcgcacca aaaatcggcg agcgcgttca
     1681 gcccggctaa gttctatcgc ttaccgatca atgtgacctt ccaagacggg gtcaacaaag
     1741 tcggcgtgca gcaacaccag tgcgcactgt gcggcgattg catgtcaggc tgcaactacg
     1801 gcgcgaagaa caccgtcctg atgaactatc tgcctgacgc gaagaatcat ggcgcggaga
     1861 tcttcacaca agtatcggtg cgttatctcg aacgaaaggg caaccgctgg cacgtgcatt
     1921 acgaaatcct cgacgttggg caagaagggt ttgaagctcc gacatcggtc gtcaccgcgg
     1981 atattgtggt tctggcagcg ggcacgctcg gcacgaatga aatcctgctg cgatcgaaag
     2041 ccgcgggctt atccttgtcc gatcaattgg gaaatcgttt ttcaggcaac ggggatgtac
     2101 tgggcttcgg ttacaacaac gacgttgaga tcaacatggt cggcttcggc agtcgcaagc
     2161 cgggccaatt cgatccggtc ggaccgacga tcatcactgc gatcgatctg cgtgagcagc
     2221 cgaatcttga agacggcatg atcatcgaag agggcggcgc gtgcgggccg ctggccgcct
     2281 tcatgccgaa gactctatcc ctcgccgcca aactcgtcgg ccaagacaca gacagcgggc
     2341 tggccgatct gatcgaggaa aactcgcgcg aactcgacag cttcattcgc ggcgcgtatc
     2401 acggcgcggt gaaaaacact caggtgtatc tggtcatggc tcacgacgac agcgggggta
     2461 acctcacgct tgaaaatgat cgcttgcaga tcaaatggcc gggcgcaggt gataaaccgt
     2521 acatcgaatc tgtgaacaac agtctagtga gagcgaccaa gccattgggc ggcacctaca
     2581 ctgaaaatcc ggcgtggagc aaactccaag cgaagaacat ggtgtcggcg catccgctgg
     2641 gtggctgcgt catggccgaa gacgcagagc agggtgtaac aaatcacaag ggtcaagtgt
     2701 tcagcggacc atccggctcg caagtctacg ctggcttata cgtcaatgac ggcgcgatca
     2761 ttccacgatc attaggtacg aatccacaca tcaccatcag cgcggtagcc gagcggaaca
     2821 tgctgctgct cgcgaaagat cgtggctgga cgatcgatta cagcttcgga caggtggcgc
     2881 agccgatcga gttgggcgtc ttcccggcga aacccggcat tcaattcacc gaagcgatgg
     2941 aaggttggtt ctcgacaaag atcaaagatg actttgaagc aggcctgcgt caaggccaag
     3001 cagacggttc atccttccgc atcatcgtga cgatccgatc ggatgacgtc gagcgcatga
     3061 tgagcgatcc cgatcatgcc gcacaactcg tcggcacggc aacagcgcct accctgtcgc
     3121 cccagccact cacgttgacg gatggcaaat tctatctatt cattcaagac cccaatcaat
     3181 tcaatgtgcg tttcatgcgg tatcgcatga agatgaccgc gcaagatgga caggtataca
     3241 ccttcgacgg caacaaagtg attcacaacg aacccggctt cgatatgtgg gctgacacga
     3301 ccacgctatt cattaccgtg tactccggcc ctgacatgaa cggcccggtc atcggtaaag
     3361 gtgttttgaa gattctgcta aaagacttgc ggcatcaatt gacgacgatg caagtgccga
     3421 acgcgaaaga cctcggccag cgcatttcaa tccttgcacg tttcggtcgc ttcttctgcg
     3481 atcaactctt tgacctctac ggtggggtct tcgctcgacc cagtgtattc gatcccggcg
     3541 ctgcgccgcg caagaaacga ccgctgcgcg ccggcgtgcc cgaagtgtat ttcttcaaga
     3601 ccgaagataa cttgcagtta cggctcaccc gatatcaagg cggcagcaaa gggccagtca
     3661 tcctcgcgca cggtggggcc gtgtccagcc tcatctattc caccgacttg atcgaaacga
     3721 acctcaccga atacttgtac gcccatggtt acgacgtgtg gctgctcgac tatcgagcaa
     3781 gcatcgaact gccttatgcg acggcgctct ataccgggga tgatatcgcc ctttacgact
     3841 ggcccgccgc agtgcagaaa gtgctggaaa gaaccggggc atccagcgtg caggccgtta
     3901 cacactgtta tggtgccatc acgttcaaca tggctatgca agccggctta cagggtgtgc
     3961 gctcagccgt gtgctcacaa atcggatcgc atccc
//
LOCUS       DASRTJ010000037         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_265769,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000037 DASRTJ010000000
VERSION     DASRTJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265769"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFF70_00705"
     CDS             <1..183
                     /locus_tag="VFF70_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43241.1"
                     /translation="GRSSMVMGWESVQVAATVTSFAEPVKPDRLGCASRAKNERLDSR
                     VAQHFQRTDAVSVSAS"
     gene            complement(767..1594)
                     /locus_tag="VFF70_00710"
     CDS             complement(767..1594)
                     /locus_tag="VFF70_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZY43242.1"
                     /translation="MIISVSSWSFHQELYAGTLRLSDVPYHAFDLGYRAVELNDMFLY
                     PRPPNRIARLLGRKAKPFVRKEYDIHALRAVRLNRFRSGTKLVCWTIDSDLTLQTAAR
                     EAQLAYLAQAIQAARFLGAPMIRVTLGGETNDRSALDRAVNLMSSVLPVAIAANIKLA
                     VENHGGLSNDPDLLIEFIERCRGGRSSALGACLDLGNFERDPLIGIQKIAPYAIHVHA
                     KALAFDDSGEEATIDYKSSLSILKAAGYAGALSIEYEGQGGAAEGIHQTRDLIEKYW"
     gene            complement(1591..2445)
                     /locus_tag="VFF70_00715"
     CDS             complement(1591..2445)
                     /locus_tag="VFF70_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY43243.1"
                     /translation="MSRYHIDSVELHTLDVGAGQPVLLLHGLGSCGDDWLFQTPVFSR
                     QYRVIAPDFRGHGSSTDSAEPITIPRLAKDMVGLMSALDISSAHVVGLSLGGLVAQQL
                     ALDYPDKVDRMVLTNTFAHLWPTSMRETLILGRRAIVSLWLPMHATARVVAADLFPKP
                     DQAELRKAIIDRVGGNNERSYRKFIQAVRRFDVRRQLPNIQAPTLVIAGERDTLVPRS
                     CQQQLVRSIPNAEWKLIQDSGHATPIDQVEKFNQVVLEFLKKDEARRLKDETQPSDSS
                     FSPQSAAL"
     gene            2589..3545
                     /locus_tag="VFF70_00720"
     CDS             2589..3545
                     /locus_tag="VFF70_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43244.1"
                     /translation="MPDSIRDRLRRILNGNPAQRIAQTDQARRSIDARRTDETIVGAT
                     SIKPRLNPIEPARSAPIDPIAPAASTPNDVTQPVSRPKVPVSEPTVPMRPIDLPAAND
                     PYQSPAVLRPIDHRMVGDVAEAQAFIENLREKMAALAEDFAAGHINRQQFESVYVHYR
                     EQRQMIEGLLTSMTSSAWRKAVAEGETAILLKRSTAKVLSYALYENDSSILLASSGDF
                     KIDPAVVVPMLSAYRSATAEAFGAGIRNSEIEGGRWLTFIPGRQTTIIVLFSIQPARG
                     QLDMLEDLHRDFETANASIISEGKGQEAAEQFVRLWTLGRGA"
     gene            3642..4136
                     /locus_tag="VFF70_00725"
     CDS             3642..4136
                     /locus_tag="VFF70_00725"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZY43245.1"
                     /translation="MATVTTQTQAKVRVLFVCTGNICRSPMAEAVFRDLVKKAKLDGQ
                     FEIKSAGTGHWHVGERPHRGTREVLLRHAIDVNGMVAKQVTHADLEYFEYIIAMDNEN
                     LSDLRSFSLNQAKASRLLDYAAGINARDVPDPYTDGRFELVYELVRIGTEGLLAKIRV
                     EKNI"
     gene            4306..>5129
                     /locus_tag="VFF70_00730"
     CDS             4306..>5129
                     /locus_tag="VFF70_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HZY43246.1"
                     /translation="MIPDNLRIEIAKRLGTLKAITPVSGGDINQAARIETDRAAYFVK
                     WNLQPLPRMFEVEARGLQLLADARAVRIPQVIAVIDEPPALVLEWIDQGHNKSDAAED
                     LGRLLAQQHRSIGKTFGLDHDNTIGANLQFNQPTEAWIDFFREYRLGVQAALARESGH
                     LPTDRARQLDWLMDHLDIWIDPKIVVPSLLHGDLWGGNYLIDAQGHPVLIDPAVYYGD
                     REAEIAFTELFGGFGSRFYAAYNEAWPLDRGYADRRDLYNLYHLLNHLNLFGEAYGG"
BASE COUNT         1232 a         1427 c         1337 g         1133 t
ORIGIN      
        1 gggcgaagtt cgatggtcat gggctgggag tcagttcaag ttgctgcgac tgtaacatca
       61 tttgcagagc cggtcaaacc cgatcggtta ggctgcgcga gcagggcaaa aaatgagcgg
      121 ctcgatagtc gagtcgctca acatttccaa cgaaccgatg cggtctctgt cagtgccagc
      181 taggtaacgc taccacccgc cgtgatatcg gtaagtgctg gatactccac caactctggc
      241 gattcataaa cttcattcat ggttgttccc tccttgccga tagtttagca tccttgaagc
      301 tcttggtcaa gccagatcga tgctctccaa ttaattaaaa aaagcggctc gatattcgag
      361 ccgcttagcc aacttgacga ttgctccaac cggaaagtcc gccgcttatg aggcgttact
      421 ggactgggcc gtgacatctg tcagcgctgc atactccatc aactctggcg attcataaac
      481 ctctttcatg gttatcacct cccgaacgtc agcttagcat ctttggaatt ttgagtcaag
      541 caggatcgat gcaatccacc taaataaaaa tccagccgct cagccaactt ggcactttct
      601 ccaattagga agaccgccgc ttatacggga atactggacg aggctgtgac gtcagtcaac
      661 ggtggatact ctaccaattt cggtgattca taaacttctt tcataaggat cacctcctga
      721 cgagcagttt agcacttctc aaatgacaag tcaagcaaaa taggatctac caatactttt
      781 caattaagtc cctggtttgg tgaattccct ccgcagcacc accctgccct tcgtactcaa
      841 tgctcagcgc tccggcgtag ccagctgcct tcaagatcga gagggaactc ttataatcga
      901 tcgtggcttc ttcgccactg tcatcaaaag ccaacgcttt agcgtgtaca tggatcgcat
      961 acggcgcgat tttctgaata ccgatcagcg gatcacgctc gaaattgccc aagtccaaac
     1021 acgcacccag tgccgacgaa cgcccgcccc gacaccgttc aatgaattcg atcaacagat
     1081 caggatcgtt actcaagcca ccgtgattct caacggctag ttttatattg gcggcgatcg
     1141 cgacgggcaa cacgctgctc attagattca cggcgcgatc gagcgccgat cgatcgttcg
     1201 tctcgccgcc caacgtgacg cggatcatcg gcgcgcccaa aaaccgtgcg gcttgaatgg
     1261 cttgggccag ataagcgagt tgggcttcgc gcgccgcagt ctgtaacgtt aaatccgaat
     1321 cgatcgtcca gcacactaat tttgtgccac tgcggaatcg attcaagcgc acggcgcgca
     1381 aggcgtgaat gtcatattct ttccgaacga acggtttggc tttgcgaccg agcaaccgcg
     1441 caattcgatt cggtggtcta ggatacagaa acatatcgtt caattcaacg gcacggtaac
     1501 ccagatcgaa cgcgtgatac ggcacatccg ataaacgcaa tgtaccggcg tacagttctt
     1561 gatggaagga ccaggaggaa acggagatga tcatagggct gcagactgag ggctgaagga
     1621 tgaatctgaa ggttgagttt catccttcag ccttctggct tcatcttttt taagaaactc
     1681 taacacgact tgattaaact tttctacttg atcgatcggc gtggcatggc cggaatcttg
     1741 aatcaacttc cattcggcat ttggaataga gcgcacgagt tgctgctgac aactgcgcgg
     1801 cacgagtgta tcccgctcac ctgcaatcac taaggttggt gcctgaatgt tgggcagctg
     1861 ccggcgcacg tcaaatcgtc gaaccgcctg aatgaacttt cggtatgatc gctcgttatt
     1921 gccgcccact cgatcgatga tcgctttccg cagctcagct tgatcgggtt taggaaacag
     1981 atcggcggcc accacccgcg ccgtggcgtg catcggcagc cagagcgaca cgatcgcgcg
     2041 gcggcccaag attagcgtct cacgcatact ggttggccac aaatgcgcga acgtattcgt
     2101 caacaccatg cgatcaactt tatccggata atcaagcgcc aattgctgcg cgaccagccc
     2161 gccgagcgat agcccgacca catgcgcgga tgaaatatcg agcgccgaca tcaagcccac
     2221 catgtctttc gctagacgtg gaatagtaat cggctcggcc gaatccgtgc tggaaccgtg
     2281 tccgcgaaaa tcaggcgcaa tgacacgata ctgccgcgag aaaaccggcg tctgaaacag
     2341 ccaatcgtcg ccgcacgaac ccaacccatg caggagcaaa accggctgac cggcgccaac
     2401 gtctaaagta tgcagctcaa ccgaatcaat atgatatctt gacatgcgac agccacccgg
     2461 ccgaatccat cgtaacacgg cggcaggcag atgacaactc gccgcttatc agcctgatac
     2521 agtcttgtaa ctcaactgtg atcgaaatgc tgtatacttt tttaatcaca tcatgactga
     2581 gttgctacat gcctgattca attcgagatc gattgcgccg cattctgaac ggcaatcctg
     2641 cgcaacgcat cgctcaaacc gatcaagccc gaagatcgat cgacgcgcgg cgcacagacg
     2701 agacaatcgt cggcgctaca tcgatcaaac ctaggttgaa tccgatcgag cccgcacgct
     2761 ccgcgccgat cgatccaatc gcacctgcag cctcaacgcc gaacgatgtt acccaacctg
     2821 tatcgcgccc gaaggtgcca gtctctgaac ctacggtgcc gatgcggccg atcgatctgc
     2881 ctgcggccaa tgatccgtat caatctccgg ccgtcttgcg cccgatcgat caccgtatgg
     2941 ttggtgatgt ggcggaagcg caggccttca tcgaaaacct gcgcgagaaa atggccgcgt
     3001 tagccgaaga ctttgccgcg ggacatatca accggcagca atttgaaagc gtgtatgtgc
     3061 attatcgcga acagcggcaa atgatcgaag ggctgttgac ctcgatgacg agcagtgcgt
     3121 ggcgcaaagc cgtggccgaa ggtgaaacgg ccatcttgct caagcgcagc acggccaaag
     3181 tgttgagcta cgcgttgtac gagaacgaca gcagcatcct gctcgccagc agcggcgact
     3241 tcaagatcga tccggctgtg gtcgtaccga tgttgtccgc ctatcgatcg gccaccgccg
     3301 aagcatttgg cgcgggcatt cgcaacagtg agatcgaagg cgggcgttgg ctgacattta
     3361 tccctggccg gcagaccacg atcattgtgc tgttctcgat acagcctgcg cgcggccaac
     3421 tcgatatgct ggaagacctg catcgcgatt tcgaaacggc gaatgcgagc atcatttctg
     3481 aaggcaaagg ccaggaagcg gccgagcaat ttgtgcgctt gtggacgctg ggccgcggcg
     3541 cttagataat ttttgatctc tgatcttcaa tctctgattt tgttcgggac ttagtcattg
     3601 atcattgcac attgttcatg gttcattcga aggactattc tatggcaact gtaactactc
     3661 aaacacaggc aaaggtgcgc gtgttgtttg tctgcactgg caacatttgt cgatcgccga
     3721 tggcggaagc ggtattccgc gacttggtga agaaggccaa gcttgacggc cagttcgaaa
     3781 tcaaatcggc cggcaccggc cattggcacg tcggcgagcg gccgcatcgc ggcacacgcg
     3841 aagtgctgct gcgccacgcg atcgatgtga acggcatggt cgccaagcaa gtgacccatg
     3901 ctgatctgga atattttgaa tacatcatcg cgatggacaa cgagaatctg tcggacttgc
     3961 gatcgttcag cctgaaccag gccaaagcat cgcgtctgct tgattacgcg gcaggcatca
     4021 atgcgcgcga cgtgcccgat ccgtatacgg atggccgctt cgagttggtg tatgaactgg
     4081 tccgcattgg cacggaaggg ctgctggcga agattcgcgt tgaaaagaat atttgatagc
     4141 gtgtcagcat cctgagcgga atggcggact cgaagagtcc gccatgcagt cgaaggacgc
     4201 gctagtagta catacgctct tcgtcaaccg cgtccttcga ctgcgagttg aagtcaatga
     4261 cttcaactct tcgcgcagga tgctgacagt actttcctca gataaatgat ccccgataat
     4321 ttacggattg agatcgcaaa aagactaggc acccttaaag ccataactcc agtcagcggc
     4381 ggagacatca atcaagcggc gcgcatcgaa accgatcgcg ccgcttactt cgtcaagtgg
     4441 aatctgcaac cactgccccg catgtttgaa gtcgaagcgc gcggactgca actcttagcc
     4501 gatgcccgcg cggtgcggat accacaagtc atcgctgtca tcgatgaacc gcccgcgctc
     4561 gttttggaat ggatcgatca aggtcacaac aaatcggatg ccgccgaaga tctcggccga
     4621 ttgctcgcgc agcagcatcg atcgattggc aaaacattcg ggcttgatca cgacaacacc
     4681 atcggcgcga acttgcagtt caaccaaccg accgaagcgt ggatcgattt cttccgcgaa
     4741 tatcgattgg gcgtacaagc tgcactcgcg cgagagagcg gccacctccc aaccgatcga
     4801 gcccggcagc tcgattggct gatggatcat ctggatatat ggatcgatcc aaaaattgta
     4861 gtgccttccc tactgcatgg cgatctttgg ggcggcaatt atttgattga cgcgcaaggg
     4921 catccagtct tgatcgatcc ggcggtgtat tacggtgatc gcgaagccga gatcgctttc
     4981 accgaactct tcggtggctt cggatcgcgc ttttatgcgg cgtataacga agcgtggccg
     5041 ctcgatcgag gctacgccga tcggcgcgac ttgtataatc tgtatcatct gctcaatcat
     5101 ctgaatttgt tcggcgaagc atacggcgg
//
LOCUS       DASRTJ010000038         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_2035,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000038 DASRTJ010000000
VERSION     DASRTJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1338
                     /locus_tag="VFF70_00735"
     CDS             229..1338
                     /locus_tag="VFF70_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43247.1"
                     /translation="MSNSIPFILMLGGDRGSQPLLEAMRRVRQACARDLLLRAKHSGA
                     FDPIIVASDDPDWTATLGDLNVTIDLDQVARPFQFGHRLADLIDRNHFDKVLYLGAAS
                     APLMTADQLIEIAEAIRTQDRVAISNNIYSSDWAAISPASIVGQWIDRLEADNSLGWV
                     LSHDAGLKPFAWPASPATRLDIDVPIDAQIAALHSDCGSNLREVVKTLQWNDHRLRAA
                     REVMRVPATRVILAGRVPSWAWAQMEKATQCWVRVYSEERGMRAAGRQNAGQVRSILN
                     DHLNSIGMKQFFIELCSMADAIVWDTRVLWAARDLWPSEIDRYASDLGLVDQITDPFI
                     REFTQAVMDAPIPIVTGGHALVSGGLWTILETMHS"
     gene            complement(1417..>2230)
                     /locus_tag="VFF70_00740"
     CDS             complement(1417..>2230)
                     /locus_tag="VFF70_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZY43248.1"
                     /translation="DLSPHTVKSFIYDLNILIEYATPARPIGQLAQRDLENFIEWLVN
                     GRGVPCNPKSLSRRLVSLKAFFRWLTEAKVILEDPAAPIAHKPVSTPLPDVLSDEQVD
                     QILAVTQKIRGAAAKPDARPHLLVTLLLTSGIKKSECMALKLEHIDLSEVEQPAIWIR
                     YTDPRNYHKERKLKLDPRWPRVFDQYQQQYRITATLFNCTSRNLEYILADVGKQAGLP
                     KPLSFEMLRWTCAVRDRSRGMKEETLRHKLGLSRVTWAETWPKIEKLAGPAI"
BASE COUNT          450 a          566 c          670 g          544 t
ORIGIN      
        1 ggtgattacg gtctgcttca accggaaatg gcgtaaggct tgctcgaagt ccgcgtcgtc
       61 gcggacgact cttagtcatg cccgcgcgga cgcgggctga tcggcataca aggatcacaa
      121 cgcgagagtc gttacggctc ccgcgtttca ttgtccaaca ggcgacggct cccttgccgt
      181 cgatcgctcg ggcgtgggag cccggccttt ctttcaagag ttcattagat gtccaattca
      241 atcccattca ttctcatgtt gggtggcgat cgtggatcgc agccgctgct tgaagcgatg
      301 cgccgcgtgc ggcaagcttg cgcgcgtgat ctgctgctcc gcgctaaaca ctccggtgcg
      361 ttcgatccca ttatcgtggc gagtgatgat cccgattgga cggccacgct cggcgatttg
      421 aatgtcacga tcgatcttga tcaagttgcc cggccgtttc agtttggcca ccgattggcc
      481 gatctcatcg atcgaaatca tttcgacaaa gtgttgtatc tgggtgcagc ttctgcaccg
      541 ttgatgactg ccgatcaact cattgaaatc gccgaggcga ttcgcactca agatcgagtt
      601 gcgatcagta acaatattta ttcttcagat tgggcagcca tttccccggc atcgatcgtc
      661 ggccaatgga tcgatcgcct cgaggccgat aattcgctgg gctgggtgct gtcacatgat
      721 gctgggctga aacctttcgc gtggcctgca tcgccagcaa ctcggttgga tattgatgtg
      781 ccaatcgatg cgcagattgc ggcactccat tctgactgcg gatcgaacct gcgcgaagtc
      841 gtcaagacat tgcagtggaa cgatcatcgt ttgcgggcgg cgcgtgaggt gatgcgtgtt
      901 ccggcgacgc gcgtaattct ggccgggcga gtgccatcgt gggcgtgggc gcagatggag
      961 aaagcgacgc agtgttgggt gcgcgtgtac agtgaagagc gcggcatgcg tgccgcggga
     1021 agacaaaatg ccggacaggt tcgatcgatc ttaaacgatc atctcaattc aattggcatg
     1081 aaacaattct ttattgaatt gtgtagtatg gccgatgcga tagtgtggga tacgcgggtg
     1141 ttgtgggccg cgcgcgacct gtggccgtcg gagatcgatc gctatgcgtc cgatttgggg
     1201 ttggtcgatc agatcaccga tccgtttatt cgagagttta cgcaagccgt tatggatgcg
     1261 ccaattccga tcgtgacggg cgggcacgcg ttggtctcgg gcggactgtg gacgattctg
     1321 gagacaatgc atagttgata atgcacaatg catgattcat tcgcggctaa atcgatctaa
     1381 atctgtgtgg gcaattgtgc attaagcatt atgcagtcaa atcgctggcc cggccaactt
     1441 ttcaatcttc ggccaggttt cggcccacgt cactcgcgac aagccgagtt tgtgccgcag
     1501 cgtttcttct ttcatcccgc gcgatcgatc gcgcaccgcg catgtccagc gcagcatctc
     1561 gaacgaaagt ggcttgggca gtccggcctg tttaccgaca tcggccaaaa tgtattcgag
     1621 gttgcgcgag gtgcagttga atagcgtggc cgtaatccga tattgctgtt gatattgatc
     1681 gaacacgcgc ggccacctgg gatcgagttt gagtttgcgc tctttgtggt agttgcgcgg
     1741 atcggtgtaa cgaatccaga tggcgggttg ttccacttcc gagagatcga tgtgctccag
     1801 cttgagcgcc atgcactcgc tcttcttaat gccgctcgtc aataataacg tgaccaacaa
     1861 atgcggacgc gcatcgggct tggctgcagc gccgcgaatt ttttgcgtga ccgctaagat
     1921 ttgatcgact tgctcatcgg acaagacatc gggcaggggc gtggagacgg gcttatgcgc
     1981 gatcggcgcg gcggggtctt caaggattac tttcgcttcg gtcaaccaac gaaaaaatgc
     2041 tttgagcgag actaagcgtc gcgataacga tttgggattg cacggcacac ctcgaccgtt
     2101 gaccagccac tcgatgaagt tttccagatc gcgttgggcc agttggccga tcgggcgcgc
     2161 gggcgtggcg tattcgatca agatgttgag atcgtaaatg aaggatttaa ccgtgtgtgg
     2221 cgaaagatcg
//
LOCUS       DASRTJ010000039         9325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_39899,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000039 DASRTJ010000000
VERSION     DASRTJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39899"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VFF70_00745"
     CDS             complement(<1..1400)
                     /locus_tag="VFF70_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43249.1"
                     /translation="MNLLRTIPIFSLCAILFGSIFMTLGTVTRSASAAPDSLPALVFV
                     TRSHLATPDDIFSDELGPAGEFGTGLPKFAPGSKLMIRHSNGSFTTLVDGTNPIAATG
                     YLIDVQSPDVSFDGTQIVFAGATTVDPESSQYGWRLYEINVNGTGFHKLNIDDRTFTS
                     VPNNNLNHYNFGDYETYGWWNDLFPAYLADGRIVFSSSRYPSRSHYDERHTYNVYVVN
                     ADGSNVHRISSERGSLLHPTPLPDGRILVARWWNNFNQPSDQGIYNRIDNQSGDHVLP
                     DGTVIYSNTDETFNPALGRLALGFELRQAPNTWHLMVIDPDGTHFQRYAFTPYSEYEL
                     TNDSGNDTYVGAQPAVVLSGTQQFIAFTSQQDESMVHSTLRTGIRVARPDVSMMYSNT
                     ADAIAGLTYDKVWGHGDTSSPYAIHPWGLPDGSILFSYAAGPIGGLPSIGSYNDPVTG
                     RNFDLQGSALQYQLYT"
     gene            complement(1540..2211)
                     /locus_tag="VFF70_00750"
     CDS             complement(1540..2211)
                     /locus_tag="VFF70_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZY43250.1"
                     /translation="MCGRFTLAVDPAQLKLLLDLNDVPADLAPRYNIAPTQLVAVVTS
                     LQDRKVEMVQWGLIPSWAKDPSIGGRMINARAETLEEKPAFRAAFKRRRCAILADGFF
                     EWKKVAEGKKETKQPYYIGLIDDQPFAFAGLWETWKSPQGEEKRTCTIITTTPNKLMA
                     EIHDRMPVILNKESIWDWIDPEASPIALQAVLQPYPAQQMKMYPISKLVNKPENDRAE
                     VITPA"
     gene            complement(2326..3003)
                     /locus_tag="VFF70_00755"
     CDS             complement(2326..3003)
                     /locus_tag="VFF70_00755"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZY43251.1"
                     /translation="MIIVFALIGLIVGGLINLLADALPDRKPLASIGSLFRWPMPIRN
                     LMVEVGTAIMFAFIYLQPVFADPIKLILAGFHMAVLILVTVTDLEHRLILNKVMFPAI
                     AVAALTSWLWFWHEYWYVAFIGGAIGFAFFWLAVVVGGRLFGRGAMSAGDVTLAAYVG
                     LITGFPGIITALVITILAGGIISVLLLLLRVVNLRSGIPYGPFLVVGGFVSILWGAAI
                     TTGFFFR"
     gene            3124..3951
                     /locus_tag="VFF70_00760"
     CDS             3124..3951
                     /locus_tag="VFF70_00760"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43252.1"
                     /translation="MLDRLTTINRERLSVFIALILISLGLLPLIESPTSGIGGSFFGT
                     PLKLEFSATTLVALLAAALSCAGIDTLLRAHPKVRRGEVARTLPYWIIPALTVVVASQ
                     ILTSITEPITWVTVLLITGALLWLECVAEYATIDAEGPAAGRARMFLNAVAYILAALL
                     FGLIWNSRARSLLSASLILLVTLGLAIDLLLSSRANGRRIFMLSAAVAIVMAEANWAT
                     NYWDTTVFIAAPAQLFIFYALVGLAGQYLIDRLNRRVLIEFAIVMLVALIVLLRGRG"
     gene            complement(4072..4254)
                     /locus_tag="VFF70_00765"
     CDS             complement(4072..4254)
                     /locus_tag="VFF70_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43253.1"
                     /translation="MARRSQSQMKAQRNLRIMFAILAILVVGSMIISAISPNVTTTTA
                     LPTSVLVRTAVPLPLK"
     gene            complement(4368..5732)
                     /locus_tag="VFF70_00770"
     CDS             complement(4368..5732)
                     /locus_tag="VFF70_00770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43254.1"
                     /translation="MNLIRVLLLFLLGVALLLVAQPHDSIAAASVERSDEYRSTYAFN
                     EADRFLQPALIRQPWNAMLYLRSSDIALGLRDLDRSSAQLDLADVAGADRSEVEVRRA
                     ILAEARQQFELAATFWLSATLDRPEDSTAILRLIDADVRLAKFDAAKTVAQDWLDRSN
                     LPEAHFALAKLIALDDPISTKDHFTQAPVDQAQEFLAALNDPQSSDPAYRSVALGRAY
                     LVDNDLQLAARAFQTATLFNPNYADAFAYYGFTLDQLGQAGGAQLDRAVEINRDLVVA
                     RYFRALHAIAQGDQSSALIDLKVAIDREPQNYLVAIELGRAYARQADFVSAEKWLQVA
                     RDLRPEDVLSWQVLCEFYSSTGANDQAIAAAQQAVVLAPNDAEAHVWLGRAHVLSGNF
                     QFAEQELRTAVRLEPRSATAHYFLGRFLKRNTDEGRLEFERASALDPTGAIGDLARRE
                     LSLP"
     gene            5861..7069
                     /locus_tag="VFF70_00775"
     CDS             5861..7069
                     /locus_tag="VFF70_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY43255.1"
                     /translation="MDFNLSAEHQMVQKMVRDFGEKEVKPTIKDWDRKQEMNPAVLPR
                     MAELGILGICIPVKYGGQGFDYISLGLACEELEAVDTSLRVIMSVHMGLNSLALLQWG
                     TEDQKQRFLVPQAKGEKYAMFGLTEPGVGSDVANMASTAKRDGSDYLINGEKMWISLA
                     TKAHHILWFAKTDVNAKDTHEGITAFMIDTSMKGVTRGDIHGKLGVRAGSTGWVNCSD
                     VRVPQENRIGAEGEGFRIAMSALDNGRYTVGSGATGLIRASLEASVKYAKERKTFGKE
                     IGKHQLIQQKIAFMVQKYEIGRLLYLRAGWMKNMGLRNTRETSLMKWYATDASFEAAD
                     EAIQVHGAYGYSDEYDVERYLRNSRGAIIYEGTSEIHQLMQAGYALGYREDGELRCEL
                     PAFDPKVWQE"
     gene            7360..8157
                     /locus_tag="VFF70_00780"
     CDS             7360..8157
                     /locus_tag="VFF70_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZY43256.1"
                     /translation="MNIVVCTKQVPDTAATLKVDEGSNVSWGDAPLVINPWDEYAIEE
                     ALRLKEKHGGKVTVLSIGPEQAKEALKQAVAMGCDEAILVWDQSLDGSDTLATSYILA
                     QAIKKIGDVDAVFFGKQAIDGDTGLVQGGVARRLGWPLLSFVSKIDALDPAAKTIEVD
                     RLVEQGHQKVASKLPAVITVVKEINEPRYPSFINIRKASKLQIPIWDAASIGVEAGKV
                     GRANAAAQWTKIMPLPARSGDVEMIGGDSPQAIASALADKLLAEKVI"
     gene            8159..9142
                     /locus_tag="VFF70_00785"
     CDS             8159..9142
                     /locus_tag="VFF70_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZY43257.1"
                     /translation="MAYNILVWIESFKGHAAALSYECLGEARRLADGGTVTAAVFGEN
                     AASIASEAITLGADKAIVCEDASLNDFRAEPYTALLTKIAIDLKPDVIMAGASTRGRD
                     LVSGLAVELETGTIADCTSIEVKDGQIVVTRPVFAGKLLATLIVPSKRPIIITTRSRA
                     FAMPMAEASHKGEVTKVNAVMSEDQIISKITGFEVEGEGEVSLNDARVIVAGGRGVGG
                     PEGFAPITALARALGAAVGASRAAVDAGWIPYTYQVGQTGKTVGPDLYIACGISGAIQ
                     HQAGMRTAKVIVAINKDADAPIFKLARYGVVGDLFQIVPALTEEFKKRLGK"
BASE COUNT         2230 a         2477 c         2603 g         2015 t
ORIGIN      
        1 atcgtataca gttgatactg aagagccgat ccttgcagat cgaaatttcg tccggtcacc
       61 ggatcgttgt aagaaccgat cgagggcagc cccccgattg ggccagcggc gtacgaaaat
      121 aagatcgatc catcgggcag accccacgga tgaatcgcat agggcgaaga agtatcgcca
      181 tgaccccaga ctttatcgta ggtcaagccc gcaatcgcat ccgcagtgtt ggaatacatc
      241 atcgagacgt caggccgcgc cacgcgaatg ccggtgcgta aagtggaatg caccatgctt
      301 tcatcctgct gcgacgtgaa ggcgatgaat tgctgcgtgc ccgataacac aacggcgggc
      361 tgcgcgccga cgtaagtgtc attgcctgaa tcattggtga gttcgtattc gctgtacggc
      421 gtgaaggcat aacgctggaa gtgcgtgcca tccggatcga tcaccatgag gtgccaggtg
      481 ttcggcgctt ggcgcaattc aaagccgagc gcgagccgac ctaaggcggg attgaaagtt
      541 tcatcggtgt tggaatagat caccgtgccg tccggtaaaa cgtgatcgcc ggattgatta
      601 tcgatgcgat tgtagatgcc ttgatcgctg ggctgattga agttgttcca ccagcgcgcg
      661 acgaggatgc ggccatcagg caagggcgtt gggtgcagca aactgccgcg ctcgctgctg
      721 atgcgatgca cattcgatcc gtcagcattc acgacgtata cgttgtaagt atgccgctca
      781 tcgtaatgcg atcggctggg atagcgcgaa gaagaaaaaa caattcggcc gtcggccaaa
      841 tatgcaggga agagatcatt ccaccagccg taagtttcat aatcgccgaa gttgtaatga
      901 ttgaggttgt tgttgggcac agacgtgaag gttcgatcgt cgatgttgag tttgtggaag
      961 cccgtgccgt tcacattgat ctcatataac cgccagccat attggctcga ttcaggatcg
     1021 acggtggtcg cacccgcaaa aacgatttgg gttccatcga acgacacatc gggcgattgc
     1081 acatcgatca agtagccggt agccgcaatc ggattggtgc catcgactaa tgtagtgaac
     1141 gatccatttg aatgacggat catcaacttg ctgcccggcg caaattttgg caggcctgtc
     1201 ccaaattctc cggcgggtcc cagctcatcc gagaaaatat cgtcaggcgt ggctaaatgc
     1261 gatcgagtga cgaagacgag agcgggcaaa ctatcgggtg cagctgaagc cgatcgggtg
     1321 accgtgccca acgtcatgaa gatcgatcca aagagtatcg cgcacagaga aaaaatcgga
     1381 atggtgcgga gcaggttcat agtcgacctt ggtttagaga tgatgacaat gctctgagta
     1441 tagcgaggac tcccaatgaa tcaatagtgc gctggtcatt cagcctaaac aaaacggcga
     1501 taggcacaaa atcctatcgc tgcatccggg tcggtcgttt taagccggcg tgatcacttc
     1561 tgcgcgatca ttctccggtt tattgaccaa cttggaaatg ggatacatct tcatctgctg
     1621 ggcgggatac ggctgcagca ctgcctgcaa tgcgatcggc gatgcttccg gatcgatcca
     1681 gtcccatatc gactctttat tcaagatcac cggcatccga tcgtggatct cagccatcag
     1741 tttgttcggc gtcgtggtga tgatcgtgca ggtgcgcttc tcttcgccct gaggcgattt
     1801 ccatgtttcc cacaagcccg caaaagcgaa cggttgatca tcgatcaagc cgatgtaata
     1861 aggctgcttc gtctctttct tgccttcagc aactttcttc cattcgaaaa agccgtccgc
     1921 caagatcgcg cagcggcgac gcttgaacgc cgctcgaaag gcgggcttct cttcgagcgt
     1981 ttcagcgcgc gcgttgatca tccggccgcc gatcgatgga tctttcgccc atgacggaat
     2041 caagccccac tggaccattt ctacttttcg atcttgcagg ctcgtcacga cggccaccaa
     2101 ctgcgtcggc gcaatgttgt agcgcggcgc aagatcagcc ggcacatcgt ttagatcgag
     2161 cagcagtttg agttgagccg gatcaacggc gagagtgaat cgtccgcaca tagtaaattc
     2221 gttttagatg gcaggttgca agttgcacga atctaatgcg tgctacttat tgcgtaacaa
     2281 atcattggac ttttacgaat tacgcaatac gccgtaataa gtttatcacc taaagaaaaa
     2341 ccccgtcgta atcgccgcgc cccacagtat gctcacgaag ccgccgacga cgaggaacgg
     2401 cccatagggg ataccgctgc gcaaattgac gacgcgcaat aagagcaaca aaacgctgat
     2461 gataccgccc gccagaatgg tgatgaccaa cgcagtgata atccctggaa agccggtgat
     2521 caatccgacg taggccgcca gggtcacatc gcctgcgctc atcgctcccc gcccgaacaa
     2581 tctgccaccc acaacgacgg ccagccagaa gaatgcgaaa ccgatcgcac caccgatgaa
     2641 ggcgacatac caatactcgt gccagaacca caaccatgag gtgagtgccg ccactgcaat
     2701 tgccggaaac atcactttgt tcagaatcaa ccgatgttcc agatcggtga ccgtgacgag
     2761 aatcaacaca gccatatgga aaccggcgag gatcaacttg atcggatcgg cgaagacggg
     2821 ctgcaagtaa ataaatgcaa acatgatcgc cgtgccgacc tcgaccatca gattgcggat
     2881 gggcatcggc cagcggaaca gtgaaccgat cgaggccagc ggtttgcgat cgggtaacgc
     2941 atcggccagc aagttgatca agccgccgac gatcaaaccg atgagggcga aaacgattat
     3001 caaactttat tccttaatcc aagatagcat gcaagggaaa taattcatcc ttacgccttt
     3061 atccttcatg agtataccac tcgctttgac aaaagcagat cgagcggcta gaatcaaacc
     3121 gctatgttag accgattaac cactatcaat cgcgaacggc tcagcgtctt tattgctctg
     3181 atcttaatca gcctcggctt gctgccgttg atcgaatcgc cgaccagcgg catcggcgga
     3241 tcgttcttcg gcacgccctt gaaattggaa ttttcagcca caaccttggt cgcgttgttg
     3301 gctgcggctt tgtcgtgcgc cggcatcgat acgttgctgc gcgcccatcc caaagtccgg
     3361 cgcggcgaag tcgcgcgcac actgccgtac tggatcatcc ctgcgttaac ggtcgtcgtc
     3421 gcatcgcaga ttctcaccag catcactgaa cccattactt gggtcaccgt attgttgatc
     3481 acaggtgctc tcttatggct ggaatgcgtg gccgaatacg ccacgatcga tgcagagggt
     3541 cccgctgctg gccgcgcgcg aatgttcttg aatgccgtgg cgtacatctt ggccgcgctg
     3601 ttgttcggcc tgatctggaa ttcgcgcgcc cgcagtttat tgagtgcctc actgatcctt
     3661 ttggtaacgc tcggcctcgc catcgatcta ttgttgagct cgcgcgcaaa cggccgccgg
     3721 atattcatgt tatcggctgc cgtcgccatc gtcatggccg aagcgaattg ggccaccaat
     3781 tattgggaca ccaccgtctt catcgccgcg cccgctcaac tcttcatctt ctatgcctta
     3841 gtcgggttgg ccggacaata tctgatcgat cggctcaatc gccgcgtact gatcgaattt
     3901 gcgatcgtta tgctcgtcgc attgatcgta ttgctgcgcg gccgagggtg atccctaaca
     3961 tcccccaggt tatacctcaa ccgatcgcgt gagcaaacaa cttgcaggag aataaccgaa
     4021 agcgaaacgc cccgatgatt tcatcgaggc gttttttgtc tggtcaacaa tctatttgag
     4081 cggcagtggc acagccgtgc gcacaagcac ggaggttggc agtgcggtgg tggtcgtgac
     4141 gttaggactg atcgccgaaa tgatcatgct gcccaccacc agaattgcca gaatcgcgaa
     4201 catgatgcgc aggttgcgct gtgctttcat ttgactttgt gaacgacgag ccatgctttt
     4261 cttactccag atctgaactg cggcgattat accattggtt tggaagttag agggatgatc
     4321 ctcttcaaca acccatccct ccaacatcca acttctatcc tccaatatta gggcagtgac
     4381 aactctcttc tcgccaaatc cccaattgcc ccggttggat cgagcgcact ggcgcgttca
     4441 aattccaaac ggccttcatc cgtattacgc tttaagaagc gtcccagaaa ataatgcgct
     4501 gttgcggatc gcggctcaag tctgaccgcc gtccgcagtt cttgttcggc aaattgaaaa
     4561 ttgccagaca acacatgggc gcggcccaac cacacatgtg cttcagcatc gttcggcgca
     4621 agcaccaccg cctgctgcgc ggcggcaatc gcctggtcgt tcgcgccagt gctgctataa
     4681 aactcgcata acacttgcca acttaacaca tcctccggtc gaaggtcgcg tgcaacctgc
     4741 agccattttt ccgctgaaac aaaatcggcc tggcgcgcat aggcccggcc cagttcgatc
     4801 gcgactaaat aattctgcgg ctcgcgatcg atcgcaactt ttagatcgat tagtgcgctc
     4861 gattgatcac cctgcgcgat ggcgtgcagc gcgcggaaat aacgcgcgac cactaaatcc
     4921 cgattgattt ccaccgcccg atcgagctgc gcgccgccag cctgtcccag ctgatcgagc
     4981 gtgaagccgt agtaagcaaa cgcatcagcg tagttgggat tgaacagtgt tgcggtttga
     5041 aatgcacgcg cggcaagttg cagatcgtta tcgaccagat acgcgcggcc taaagcgacc
     5101 gatcgataag ccggatcgct tgactgtgga tcattcaatg cggccagaaa ctcttgcgct
     5161 tgatcgaccg gcgcttgcgt gaaatgatct ttagtgctga tcggatcatc gagcgcgatc
     5221 agtttggcta gcgcgaaatg cgcttccggt aagttcgatc gatcgagcca gtcctgcgca
     5281 actgttttgg ctgcgtcgaa tttagctaga cgaacatcgg catcgatcaa acgtaatatc
     5341 gccgttgaat cttcgggacg atcgagtgtg gcacttaacc aaaaagtggc ggctaattca
     5401 aattgctgcc gggcttcggc cagtatggct cggcggactt cgacttccga tcgatctgca
     5461 ccagcgacgt cggccagatc taattgggcg gaagatcgat cgagatcacg caagcccaat
     5521 gcaatgtcac tcgatcgtag atacagcatt gcattccatg gctgccggat caacgcgggt
     5581 tgcaagaatc gatcggcttc attgaaggca tacgttgatc gatattcatc gctgcgctca
     5641 acggacgccg ccgcgatcga atcatggggc tgagccacga gcagcagcgc cacaccgagc
     5701 aagaataaca gcaaaacccg aatgaggttc attggtgtag aattatactc gctaacgggt
     5761 tatgagtcgt gcggtatcga gcgatactgc attgcaacgc ccgcagagtg gtgcaagttc
     5821 tacttgatct tgttctttat cacttttcaa ggagcctctc atggatttca acctctcggc
     5881 cgaacatcaa atggtccaaa aaatggtgcg cgactttggc gagaaagaag ttaagccgac
     5941 gatcaaagat tgggatcgca aacaggaaat gaatccggcg gtcttgccgc gcatggccga
     6001 gttgggcatc ttgggtattt gcattccggt gaaatacggc ggccagggct tcgattacat
     6061 ttcgctcggc ctcgcctgcg aagaattgga agcggtcgat acgtcgctgc gcgtgatcat
     6121 gtcggtgcac atgggcttga atagtttggc gctgctgcaa tggggcacgg aagatcagaa
     6181 gcaaagattt ttagtgccgc aagcgaaagg cgaaaaatat gcgatgttcg gtctgaccga
     6241 gccgggcgtc ggcagcgatg tggcaaatat ggcctcgacc gcgaaacgcg acggcagcga
     6301 ttacctcatc aacggcgaga agatgtggat cagtttggca acgaaggcac atcacatttt
     6361 gtggttcgcc aaaacggacg tcaacgccaa agacacgcat gaagggatta cggccttcat
     6421 gatcgatacg agtatgaaag gcgtgacgcg cggcgacatt cacggcaagc tcggcgtgcg
     6481 tgccggatcg accggctggg tgaattgtag cgatgtgcgc gtgccacaag aaaatcgcat
     6541 cggcgcagaa ggcgaaggtt tccgcatcgc gatgtcggcg ctcgataacg gtcgatatac
     6601 cgtggggtcg ggcgcgacgg gattgatcag agcctcgctc gaagcgagtg tgaagtatgc
     6661 gaaggagcgc aagacgttcg gtaaagagat cggcaagcat caattgatcc agcagaagat
     6721 cgcgttcatg gtgcagaagt atgagatcgg ccgattgttg tatctgcgcg cgggttggat
     6781 gaagaacatg ggcttgcgca acacgcgtga gacttcgctg atgaaatggt acgcgactga
     6841 tgcgtccttc gaagcggccg acgaagcgat ccaagttcac ggtgcgtatg gttactccga
     6901 tgaatacgat gtggaaagat atttacgaaa ttcgcgcggc gcgatcatct acgaaggcac
     6961 gagcgagatt catcaattga tgcaggcggg ttatgcgcta ggctatcgag aggacggcga
     7021 gttgaggtgt gagttgccgg cgttcgatcc gaaagtttgg caggagtagc gagtcagcga
     7081 atcagcgaaa aacttaaccg cgaccctgca cactacacgc aaggcaggcg acgctaagac
     7141 gcaaagagca attcaatttg tcattccgaa cgaagtgagg aatctctatc tcaattggcc
     7201 tcgtgagttt gcagtggcag agattcctcg gcctatggcc tcggaatgac acgcaaattt
     7261 aaagaaaatc cacatgctat aattgcgctc ggtatctagc cacatgcgat aggctagttg
     7321 ctattagcca actgctatca ttcttaagga ggtttcccgt tgaacattgt cgtctgcacc
     7381 aaacaagtcc ctgataccgc ggcgacgctc aaggtcgatg aaggatcgaa cgtgagctgg
     7441 ggagacgcgc cgctggtcat caatccgtgg gacgagtacg caatcgaaga agcgctccgc
     7501 ttgaaagaga aacacggcgg caaagtcacc gtgctctcga tcgggccgga gcaagccaag
     7561 gaagcgttga agcaagcagt agccatgggc tgcgatgaag cgatcctcgt gtgggatcaa
     7621 tcgctcgacg ggagtgacac gctggcgacg agttacattc tcgcgcaagc gatcaagaag
     7681 atcggcgatg tcgatgcggt gttcttcggc aagcaggcga tcgatggcga taccggcctt
     7741 gtgcagggcg gcgtcgctcg acggttgggt tggccgctgc tgtcgtttgt gtcgaagatc
     7801 gatgcgctcg atccagccgc gaagacgatc gaggtcgatc gcttggtgga gcagggtcat
     7861 caaaaagtag cgagtaaact accggccgtc atcaccgtcg tcaaagagat caacgagccg
     7921 cgttatccat cgttcataaa catccgcaaa gcgagcaaac tacaaattcc gatttgggac
     7981 gccgcatcga tcggtgtgga agcaggtaaa gtcggtcgag cgaatgcggc ggcgcaatgg
     8041 acgaagatca tgcccctgcc cgcgcgcagc ggcgacgtcg aaatgatcgg tggcgactcg
     8101 ccgcaagcga tcgcatcggc gttggcagac aaattgctgg cggagaaggt gatctaacat
     8161 ggcatacaac attcttgttt ggatcgaatc cttcaaaggc catgcggctg cactatctta
     8221 cgaatgttta ggcgaagcgc gtcgattggc cgacggcggc acagtgaccg ccgcagtctt
     8281 cggcgagaac gcggcctcga tcgcaagtga agcgatcaca ttgggcgccg ataaagcgat
     8341 cgtatgtgaa gatgcgtcat tgaatgattt tcgggccgag ccatacactg ccttgctgac
     8401 caagatcgcg atcgatctca agcctgatgt catcatggcg ggcgcatcga cccgcggccg
     8461 cgatctggtc agcggcctcg ccgtcgagtt ggaaaccggc acgatcgcag actgcacctc
     8521 gatcgaagtg aaggacggac agatcgttgt gacgcggccc gtcttcgcgg gtaaattgct
     8581 agcaacgctg atcgttcctt ctaaacgacc gatcatcatc acgactcgat cgcgtgcgtt
     8641 tgcgatgccg atggctgaag cttcgcataa gggcgaagtg acaaaagtga atgcggtgat
     8701 gagtgaagat caaatcatca gcaaaatcac cggtttcgag gttgaaggcg aaggcgaagt
     8761 cagcctcaac gatgcgcgcg tcatcgtagc gggcgggcgc ggtgtcggcg gaccggaagg
     8821 cttcgcgccg atcaccgcct tggcgagagc acttggcgcg gcggtcggag ccagtcgagc
     8881 ggcggtcgat gcaggttgga ttccctacac gtatcaagtc ggccagaccg gcaagaccgt
     8941 tggccccgat ctgtacatcg cctgcggcat cagcggcgcg attcaacatc aagccggcat
     9001 gcgcaccgcc aaagtgatcg tcgcgatcaa caaagatgcc gacgcgccga tcttcaaact
     9061 cgcgcgttac ggcgtcgtcg gcgatctatt ccagatcgtt ccggcgttga ctgaagagtt
     9121 caagaagagg ctcggtaaat agcctcatcc aatgaactaa ctacggagct acacacctgc
     9181 ggtaaagcac gcagtgcgga ccggaggaca cggagaaaaa taaaaactct gtgcctccgt
     9241 gtctctgtgg ttttattttt cagctctttc actgactcgc tgattcgctc cttcgacttc
     9301 gacatgtctc ctgcggattc atgtc
//
LOCUS       DASRTJ010000040         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_265852,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000040 DASRTJ010000000
VERSION     DASRTJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265852"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1560)
                     /locus_tag="VFF70_00790"
     CDS             complement(<1..>1560)
                     /locus_tag="VFF70_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HZY43258.1"
                     /translation="YLADRAWGTVREDYSANGDAWNYLSHDQSRSRAYRWNEDGIGGI
                     CDRMQYLCFGLALWNGKDPILKERFFGLSGPEGNHGEDVKEYYFYLDSTPTHSYMKML
                     YKYPQAEFPYWKLIDENKQRDRNSFEYELLDTGVFNDSRYFDVFIEYAKAAPDDILIK
                     ISIANRSPEAAELDVLPQLWFRNEWSWGKQDHPKPQLRAINETTIHANHAELGERWLY
                     CNNGRVQGLGQAQGLPLLLFTENESNKERLWSAANDSPYVKDAFHDYVVNGKTNRVNS
                     DRTGSKAAAQYHLTIDAGQTIMLTLRLSDKYTNRPFDDFDSIFATRIKEADEFYRAIQ
                     APALSDDEKNVQRQAFAGLMWSKQFYHYNVERWLYGDPIGPPPPIERQKGRNHEWTQV
                     DSRDIISMPDKWEYPWFAAWDLAFHTIAIALIDPEFAKRQLLLMLREWYMHPNGQIPA
                     YEWEFGDVKPPVHAWAAWRVYKIDKKHTGVADTDFLERIFHKLLLNFTWWVNRKDAEG
                     NNVFQGGFLGL"
BASE COUNT          336 a          443 c          395 g          386 t
ORIGIN      
        1 cgaggccgag aaaaccgcct tgaaaaacat tattgccttc cgcgtctttg cgattcaccc
       61 accacgtgaa gttcaacaat aatttgtgga agatgcgttc cagaaaatcg gtatccgcga
      121 cgccagtgtg cttcttatcg atcttataca cgcgccacgc ggcccaggcg tgcacgggcg
      181 gcttcacatc gccgaattcc cattcgtacg cagggatttg tccgttgggg tgcatatacc
      241 attcgcgcag catcagcagc aattgccgct tggcaaactc cggatcgatc aacgcgatgg
      301 cgatggtgtg aaaggccaag tcccacgcgg cgaaccacgg atactcccat ttatccggca
      361 tcgatatgat gtcgcgcgaa tcgacctggg tccattcgtg attgcggcct ttctgccgct
      421 cgatcggcgg gggcggcccg atcggatcac cgtacagcca acgctcgaca ttgtaatgat
      481 aaaactgttt gctccacatc aatccggcaa aggcttgacg ctgcacgttc ttttcatcgt
      541 cgcttaacgc tggcgcttga atcgctcgat agaactcatc cgcctctttg atgcgcgttg
      601 caaagatcga atcgaaatca tcgaatggcc gattggtata cttgtccgat agtctcagcg
      661 tcaacatgat cgtttggccg gcgtcgatcg ttaagtgata ttgtgccgct gctttgctgc
      721 ctgtccgatc ggagttcacg cggttcgttt tgccgttaac aacgtagtcg tggaacgcat
      781 ccttcacata tggcgaatcg ttggcggcgc tccacaagcg ttctttgttc gattcgtttt
      841 cagtgaataa cagcaggggc aagccctgtg cttgccctaa gccttgtact cgcccgttgt
      901 tgcaatacaa ccaacgctcg ccgagttcgg catgattcgc atgaatcgtc gtttcgttga
      961 tcgcgcgcag ctgcggcttg ggatggtcct gtttgcccca cgaccactcg ttgcgaaacc
     1021 acaactgcgg cagcacatcc agctcggccg cttccgggct gcgattcgcg atcgagattt
     1081 tgattaagat gtcatcgggc gcggctttgg catattcgat aaacacgtcg aagtagcggc
     1141 tgtcattgaa cacgcccgta tcgagcaact cgtactcaaa tgaattccga tcgcgctgct
     1201 tgttctcgtc gattaacttc caatacggga actccgcctg cggatatttg tacagcatct
     1261 tcatataaga atgggtcggc gtactgtcga ggtaaaaata atattccttc acgtcttcgc
     1321 cgtgattgcc ctcggggccg ctcagaccga agaaacgttc tttgagaatg ggatctttgc
     1381 cgttccacaa tgccaaccca aaacacagat attgcatgcg atcgcaaatg ccgccaatgc
     1441 catcttcatt ccagcgatag gcgcggctgc gcgactgatc gtgcgacaga taattccacg
     1501 cgtcgccatt tgcgctgtaa tcttcgcgca ccgtccccca ggcgcgatcg gccagatacg
//
LOCUS       DASRTJ010000041         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_341571,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000041 DASRTJ010000000
VERSION     DASRTJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341571"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFF70_00795"
     CDS             <1..347
                     /locus_tag="VFF70_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZY43259.1"
                     /translation="TEMTFHDPCYLGRHNNVYDAPRQVLYSVGAEIVEMDRNRNNSFC
                     CGAGGAQMWKEEEYGTERVSANRIKEAMATGKDTLAVGCPFCMIMLTDAAKAEQTAMQ
                     IKDVAEIVAERI"
     gene            489..1427
                     /locus_tag="VFF70_00800"
     CDS             489..1427
                     /locus_tag="VFF70_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY43260.1"
                     /translation="MANQIETISLGKTDVKVTPLGLGAWQWGDRFMWQYGNGYNDRDV
                     HDAFKVSLDASINWIDTAELYGSGTSERLLGQFMHELNVQPLVATKFLPLPWRVRQGS
                     LVDALKRSLNRLNLSKVDLYQIHFLLPFRSVETWANGLADALDAGLTRAVGVSNYNVE
                     KTLKTVNVLAKRGKVLASNQVEYSLLNRSIERSGLIDLCRELEITVIAYSPIGKGLLS
                     GKYTPDHLPPGRRGRKYNRAYLIKIQPLIDLIKQIGQGHGGKTPAQIALNWVLCKGAV
                     AIPGAKNGMQAHENAGALGWRLTNDEVSRLDEASDQ"
     gene            1595..>1988
                     /locus_tag="VFF70_00805"
     CDS             1595..>1988
                     /locus_tag="VFF70_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY43261.1"
                     /translation="MTDISRVQAVAQSIQANIGRVIVGKSSVIELLLAALFSEGHVLI
                     EDVPGLGKTMLAKSLAKSLGCSFQRVQFTPDLLPSDITGVNVFNQKSGDFEFRPGPIM
                     AQIVLADEINRAGPRTQSALLEAMEERQV"
BASE COUNT          483 a          470 c          586 g          449 t
ORIGIN      
        1 ctactgagat gacttttcac gatccatgct atctcggtcg gcacaacaat gtgtacgatg
       61 cgccgcgcca ggtgctgtat tcggtcggcg cagagatcgt cgagatggat cgcaatcgca
      121 acaattcgtt ttgttgcggc gcgggcggcg cgcagatgtg gaaagaagaa gagtacggca
      181 ccgaacgtgt cagcgcgaat cgcatcaaag aggcgatggc gaccggcaaa gatacgttgg
      241 cggtcggttg tccgttctgc atgatcatgt tgaccgacgc agccaaggcc gagcagacgg
      301 cgatgcagat caaagatgtg gcggagattg ttgcggagag aatttgatcc acgaagggca
      361 cgaaaagttg atctgtcatt ctgagaagcg aagcaacgag gaatctctat cactgtttag
      421 attgattgtt tatggaggta gagattcctc gctgcgctcg gaatgacaag agtgcatttt
      481 ggagattaat ggcgaatcaa atagaaacaa tatccttagg caagaccgat gtaaaagtta
      541 cgcccctcgg cctcggcgcg tggcagtggg gcgatcgatt catgtggcag tatggcaacg
      601 gctacaacga tcgcgatgtg catgacgcgt tcaaggtctc gctcgatgca agcatcaact
      661 ggatcgatac ggcggaactg tatggcagtg gcacgtcgga gcgattgctg ggtcagttca
      721 tgcatgagtt gaatgtccag ccgctcgtcg cgacgaagtt cttgccgttg ccatggcgtg
      781 tgcggcaagg ttcacttgtg gacgcgctta aacgcagttt gaaccgattg aacctctcga
      841 aagtagactt gtatcagatt cactttctct taccatttcg atccgtcgaa acgtgggcga
      901 atgggctggc ggacgcgctc gatgcggggc tgacgcgcgc ggtcggcgtg tcgaactata
      961 acgttgagaa aactcttaag accgtaaatg ttttggccaa acgcggcaaa gtcctggctt
     1021 caaatcaagt agagtattcg ttgctgaatc gatcgatcga acgaagcggt ttgatcgatc
     1081 tatgccgcga gttggagatc acggtgattg cctacagccc gatcggtaag gggctgctca
     1141 gcggcaaata cacacctgat catctgccgc cgggccgtcg aggtagaaaa tacaatcgag
     1201 catatctgat caagattcag ccgttgatcg atctgatcaa acagatcgga caaggacatg
     1261 gcggcaagac acccgcgcaa atcgcattga attgggtgct ctgcaaaggt gcagtcgcca
     1321 tccccggtgc gaaaaatgga atgcaagcgc atgagaatgc gggcgcattg ggttggcggt
     1381 tgacgaatga tgaagtgagc aggcttgatg aagcaagtga tcagtgatca ggatggcgcg
     1441 ttataaagtt gcttgtcatt tcgagccgcg ctttttgcgg cgagaaatct ctatctcaat
     1501 tggctgtgat tgtaaatcga gatagagatt tctcgcttcg ctcgaaatga catagcccac
     1561 aggccggctc aacaaatcag aaatcagaga ttccatgacc gacatcagcc gtgttcaagc
     1621 agttgcccaa tcaatccaag ccaacattgg ccgcgtcatc gttggcaagt cgagcgtgat
     1681 tgagttgctg ttggccgcgt tgttcagtga gggccacgtc ttgatcgaag atgtgcctgg
     1741 cctgggcaag acgatgttgg cgaagtcgct ggcgaaatcg ctcggctgct catttcagcg
     1801 cgtgcagttc acgcccgatc tgctcccgtc cgatattacg ggcgttaacg tcttcaatca
     1861 aaagagcggc gactttgaat ttcgacccgg cccgatcatg gcgcagatcg ttttggccga
     1921 tgagatcaat cgagccgggc cgcgcacgca atcggccttg ctcgaagcga tggaagagcg
     1981 ccaagtct
//
LOCUS       DASRTJ010000042         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_229153,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000042 DASRTJ010000000
VERSION     DASRTJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229153"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFF70_00810"
     CDS             complement(<1..502)
                     /locus_tag="VFF70_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43262.1"
                     /translation="MSLIIVGLILVAPLLQSSPLQTDDGALHIYRTVALDRAIHDGLI
                     YPRWFPDLAFGYGFPFFNYREPLGYYLIELPHLIGLSIPLALNLLLAGSVILSGLAVM
                     WWVSDIFNQRAGFVAGVLYMASPYTLIGPIERGNLPEVIALVLIPLVLWAIRRVIVLG
                     GRKYFIA"
     gene            complement(613..3876)
                     /locus_tag="VFF70_00815"
     CDS             complement(613..3876)
                     /locus_tag="VFF70_00815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43263.1"
                     /translation="MIPFIRRHSIFFSALIIFVLAFITRAGALKQYVTPDEPIWVLRS
                     LNFATALSRGDWAGTAQIGHPGVTTMWLGSIGIVLKRLADPIASVQAIDWLRQVPALA
                     PDNAEAFKRLGVFLTFARLPVILINTLGIVGIFLLAQKLFGQSMALLAALLMALDPFM
                     AGLSGLLHVDGLLTTFSTLSVLSLLIAISGQRSAISRESSVAVRPRSAVRWFALSGFF
                     AALAFLSKSPALFLTPFTFLVVSIAILFKMIPLRSALIGLSTFVICHFAFVILLYPAM
                     WLNPIGTLQGILGLASFYSGNAVRPTFFDGQYVLNHGLTFYPTALLYRLSLIELLGLI
                     IAIVFIVREIRRRRSNFQLPASNLYSIVIFLVYAILFIVFITPVAKKYDRYMLPVFPM
                     LIFVAAWGIGQLSQITLKTFRFGSVFKYLLPMAVAAQVIMVAINWPYLLMHYDPLLGG
                     SVEAQKHFAVGWGEGLGAAANWINSQPDGLQSTVATAAIPSFAPIFSGRSTGINDRGL
                     ELADYSVLTLSERQLNPNAFAQLQARATIVQTLHLGRIEGAWIFANNFAQQQAEVLNQ
                     ADAQQDAIITSIDLPVSRAYHGAAKMVVLRADVTPKQIELILNDLSTHYRRLWFTWST
                     ASMPVVQNQIQHWLSQTADLASQQSVGAVQLAAYDLRPGKIGQIDPFITQFNGNFLLL
                     GITPSPARVQAGQSSDVVMRWQATASVNSVYTATLELVDGQGEVWSAGGDPITDQDQL
                     LTPAWPIGHIAEQFFDLSLPREIPPGQYAVRVSIEQSNHSVGLFSPSGTFSGTAPMLA
                     TLQVDRNDQPLTSIGRTIEYPFSHTWQNAITLVGFDNGPGVVINGDPWTVNVVWQAVM
                     ANLPKLKVLWQVRGPDNALVYETRLPLSPYSTTNWRAGEIIGAHYTLRFPAELPNADY
                     HVSLGVIDPAGQLSAGGMFTPFDVRLLHRDRSFDLPPPQYPISITFSDPAITLIGADY
                     SATTLHAGDQLPLTLYWQAAATTDTRYNVFVHFETLDGRVITQIDSQPQGSGMPTTSW
                     APGQSILDVYPFTLPADTPPGAYQIVVGMYNPLDGEHLINQATGLDHVVLSPPIIIR"
     gene            complement(4040..>6172)
                     /locus_tag="VFF70_00820"
     CDS             complement(4040..>6172)
                     /locus_tag="VFF70_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43264.1"
                     /translation="GLANAIGLIADRLKNRARESSSLSGASLFARGVVKGRDLNQAPR
                     PSTAQRSFDSLFRSGCFYIFVVACFIITSIQFIGVWPLTLRAYNPLLGGFNAASRALP
                     VGGGESAAAGSALAASPFASKVIAASDIIGTAPFFPGDLVPNDEAGFARADYLLFNTS
                     DFQLTPDVPQHWIDAAPVLTISIQNQNFAWLYPNQRLAADRQRLIDQRQVGDALITDY
                     LATLPARTEDPTIVLPEDISEATAIDQLNRVAQSHARIFFFHYTASRKPTSILITRLL
                     DTFAIKLDDWSSALSSGALYALPKGVSFNSTPTPLNAKATFGQRAHLTEAALSMPHVQ
                     PGQSIGLATQWIAALPDTQLFVSLIDADGHTWSQIDDRVPASDSGNTQRAKRLTVPVP
                     PVTPPGAYQLKLSLIDLASGAPLSIQIADNNFAGFDWLLGSITIDPAHTQIDPATRKP
                     AIEIDADLNGLRAIGSDMPPDPIVSGDPWTLSMEWASSAEHLPELDIEWQLVSNNQIV
                     YSTTLPLNSYATDHWRTGDVLQSKYDFRLPITLLAGKYDLKFKLIDHATAQVLDDQVV
                     RLTSVNISSRPRDFTAPPVQYSSNIQFGSTATLLGANRLQADQALTVTLVWRANQITT
                     TNATAFVQIINGDQVVQQIDNWQIGGDAPTSTWAPGQIIVDQYVFDVSSGNYQVAVGL
                     YDAANGQRLPAIDAAGKRLPQDRAFVIK"
BASE COUNT         1496 a         1626 c         1790 g         1260 t
ORIGIN      
        1 acgcgataaa atattttcgt ccacctaaaa cgatcactcg acggatagcc cacagcacca
       61 atgggatcaa caccaacgcg atcacttccg gcagattgcc gcgttcgatc ggcccaatca
      121 aagtataggg cgaggccata tataagacac ctgcgacaaa gcccgcgcgc tgattaaaga
      181 tgtcgctgac ccaccacatc actgctaagc cgctcaagat gacgcttccg gctaatagta
      241 aattgagagc cagcggaatg ctcaagccga tcaggtgcgg cagctcgatc agataataac
      301 ccagcggttc gcgatagttg aagaagggaa agccgtaacc gaaagccaga tcaggaaacc
      361 agcgtggata gatcaagccg tcatgaatcg cccgatcgag cgcgacggtc cgatagatat
      421 gcagcgcgcc gtcatcggtc tgcaagggcg atgattgcaa taacggtgcg acgaggatca
      481 ggccgacaat gatcaatgac aaatgacaaa tgacagatga caaacgcggc gaggggggac
      541 ccgtctctac ttcgttacga gggcgcttcg cggaggcaag aggtgtcatc gaatagtcaa
      601 cgaatcaacg aatcagcgaa tgataatcgg tggactgagt acaacgtgat caaggccggt
      661 ggcttgattg atcaagtgtt cgccgtcgag tggattatac atgcccacga cgatctgata
      721 agcgccgggc ggtgtgtcgg cgggaagggt gaacggataa acatccagaa tggattgtcc
      781 gggcgcccat gaagtggtgg gcatgccgct gccctgcggt tgcgaatcga tttgagtgat
      841 gacgcggcca tccagcgttt cgaaatggac gaaaacgtta tagcgtgtat cggttgtcgc
      901 cgcggcctgc caatataaag tgagcggtag ttgatcacca gcgtgaagtg tcgtcgccga
      961 gtaatccgcg ccgatcaacg taatcgccgg atcgctgaat gtgatcgaga tcggatactg
     1021 cggcggaggc agatcgaacg atcgatcgcg gtgcaataat cgcacatcaa atggcgtgaa
     1081 catgccgcct gctgataatt gaccagctgg atcgatcacg cctaatgaga catgataatc
     1141 ggcgtttggc agctcggccg gaaaacgcag cgtgtaatgc gcgccgatga tttcaccggc
     1201 gcgccagttg gttgttgaat acgggctcag cggcaaacgt gtttcgtaaa cgagcgcgtt
     1261 gtctgggccg cgcacttgcc acagaacttt taattttgga agattggcca tcaccgcctg
     1321 ccacacaaca ttcaccgtcc atggatcgcc gttgatgacg acccccggcc cattatcaaa
     1381 accgactagc gtgattgcgt tttgccacgt atggctgaat ggatactcga ttgttcgccc
     1441 gatcgaagtc aacggctgat cgttgcgatc gacttgcagc gtggccaaca tcggtgcagt
     1501 gccagagaat gtgcccgacg ggctgaataa gccgacactg tgattcgatt gttcgatgct
     1561 gactctgact gcgtactggc ctggcggtat ttcacgcggc aggctgaggt caaaaaattg
     1621 ttcagcaata tgcccgatcg gccacgcggg cgttaagagt tgatcttgat cggtaatcgg
     1681 gtcaccaccg gcgctccaga cttcaccctg cccgtcgact aattccagcg tggcggtata
     1741 gacgctattc acggaggctg tggcttgcca gcgcatgacg acatcactgg attgtcccgc
     1801 ctgcacgcgg gcgggagagg gcgtgatgcc caataacaag aagttgccat taaattgcgt
     1861 gatgaacgga tcgatctgcc cgatctttcc gggccgcaaa tcataagccg caagctgaac
     1921 cgcgccgaca ctttgctgcg aagccagatc ggccgtttgg gacagccagt gttgaatttg
     1981 attttggacc acgggcatcg atgcagtcga ccaggtaaac cacagccgcc gataatgcgt
     2041 gctcagatcg ttcaggatca attcgatctg cttgggtgtg acatcagcgc gcagcaccac
     2101 catcttggct gcgccgtgat aggcccgcga taccggcaga tcgatcgatg tgataatcgc
     2161 gtcttgctgt gcatctgctt gattcaaaac ctcggcttgt tgctgcgcga aattattggc
     2221 gaagatccag gcgccttcga tgcggccgag gtgcagcgtc tgcacgatcg ttgcgcgcgc
     2281 ctgtagctgg gcgaatgcat tcggattcaa ctgccgctcg ctcagggtga gcacagagta
     2341 gtcagccaac tccaaacccc gatcgttgat cccggttgat cgaccgctga agatcggtgc
     2401 gaaggacggt atggccgccg tcgccaccgt cgattgtaaa ccatcgggct gggagttgat
     2461 ccaattcgcc gccgcaccca ggccttcacc ccagcccacc gcgaaatgtt tttgcgcttc
     2521 aacgcttccg ccgagcagcg gatcataatg catcaacaaa tacggccagt tgattgccac
     2581 catgatcact tgcgcagcga cggccatagg cagcagatat ttgaatacac tgccgaagcg
     2641 gaatgtcttg agtgtgatct gagatagttg tccaatgccc caggccgcga cgaagattag
     2701 catcgggaag acgggcagca tatatcgatc gtacttcttg gcgacgggcg tgatgaagac
     2761 gatgaaaaga attgcgtaga cgagaaaaat gacaatggaa tagaggttag aggctggaag
     2821 ttggaaattg gatcggcgcc gacggatttc gcgcacgatg aaaacaatag cgatgatcaa
     2881 gccaagtagt tcgatcagcg agaggcgata cagtaaggct gtcggataaa acgtcaagcc
     2941 gtgattaagc acatactgcc cgtcgaagaa tgtggggcgc accgcattgc cgctgtagaa
     3001 agaagccaga ccgaggatgc cttgcagcgt gccgatcgga ttgagccaca tggcggggta
     3061 cagcaaaatg acaaaggcaa aatgacaaat cacaaatgta gaaagaccga tgagcgctga
     3121 tcgaagcgga atcatcttga acaagattgc gatgctgaca actaagaaag tgaaaggcgt
     3181 taagaacaat gcgggtgact tacttaggaa ggcgagtgcg gcgaagaagc ccgataacgc
     3241 gaaccacctg accgccgacc gcggacgaac ggccacagag gattcacggc tgatcgctga
     3301 gcgctgaccg ctgatcgcga tgagcagcga caaaaccgac agagttgaaa atgtggtcag
     3361 caatccatcg acgtgcagca agccgctgag tccggccatg aacggatcga gtgccatcaa
     3421 tagtgcggcc agcagcgcca tcgactgccc gaataatttt tgggcgagca agaaaattcc
     3481 gacgatgccc agcgtgttga tcaaaatgac tggcagtcga gcgaaagtga ggaacacgcc
     3541 gagccgcttg aaggcctcgg cgttgtcagg cgcgagcgca ggcacctgcc gcagccaatc
     3601 gatcgcttgg acggacgcga tcggatcagc caaacgtttc aacacgatgc cgatcgatcc
     3661 cagccacatc gtggtgacgc ccgggtgtcc gatctgcgcg gtgcctgccc aatcgccgcg
     3721 gctcaacgcc gtcgcaaagt taagcgagcg cagcacccag atcggttcat cgggcgtgac
     3781 gtattgtttg agcgcgcccg cgcgcgtgat gaacgcgagg acaaagatga tcagcgcgga
     3841 aaaaaagatc gaatgacggc ggatgaatgg gatcatgaga ggccaagccg tcattgcgag
     3901 gaggccgtta ggccgacgaa gcaatctcag tggattgtcg tggagattgc ttcgtcgcgc
     3961 ctccgacttc ctcttcgccg cgctttgcgc gcgaagagtt cgctcaggat gcttctcgca
     4021 atgacgaagg gaattgattt cactttatca cgaatgcgcg atcttgcggc aaacgcttgc
     4081 ccgctgcatc gatcgcgggc agacgttgac cattcgctgc gtcatatagg ccaaccgcga
     4141 cttggtagtt acccgatgat acatcaaaca catattgatc gacgatgatc tgtccgggtg
     4201 cccaggtgct ggtcggcgcg tcgccgccaa tttgccaatt gtcaatctgc tgcacgactt
     4261 gatcgccgtt gatgatctgc acgaacgcgg tggcgttggt agtcgtgatt tgattcgctc
     4321 gccagactag tgtcactgtc agcgcttgat cggcttgaag cctatttgcg ccgagcaaag
     4381 tggctgtcga tccaaattga atgttgcttg aatattgaac cggcggtgcc gtaaaatctc
     4441 gtgggcgtga cgaaatatta acggatgtta aacgcacaac ttgatcatcc agcacttgag
     4501 ccgtcgcatg atcgatcaac ttgaacttta gatcatactt gccagccagt aatgtaatcg
     4561 gcaaccgaaa atcgtatttg ctttgcagca catcgcctgt gcgccagtga tcggtggcat
     4621 aggaattgag cggcagcgtg gtcgagtaaa cgatctgatt gtttgaaacc aactgccact
     4681 cgatatctaa ctccggcaga tgttccgcgc tcgacgccca ttccatcgac aacgtccacg
     4741 gatcgccgct cacaatcgga tcgggcggca tatccgatcc gatcgcgcgc aggccgttga
     4801 gatcggcatc gatctcgatc gcgggcttgc gcgtggccgg atcgatctgc gtatgcgccg
     4861 gatcgatcgt gatcgagccg agcagccaat cgaatccggc gaaattgttg tccgcaattt
     4921 gaatgctgag cggcgcgccg ctcgccagat cgatgaggct taacttcaat tgataagcac
     4981 ctggcggtgt gacgggcggc acaggcacgg tcagacgctt cgcgcgctgc gtgttgccgc
     5041 tgtcactggc gggcacacga tcatcgatct gactccaggt atgtccgtca gcatcgatca
     5101 aggacacaaa caattgtgta tcaggcagcg ccgcgatcca ttgagtggcc agaccgatcg
     5161 attggccggg ctgcacgtgt ggcatgctca acgctgcctc tgtcaagtgt gcgcgctggc
     5221 cgaaggttgc cttcgcattc aaaggtgtcg gcgttgagtt gaatgaaaca cctttgggca
     5281 gtgcatacaa cgcgccgctg ctcaaagctg aggaccagtc atccagtttg atcgcaaatg
     5341 tatcgagcaa acgtgtgatc aaaatgctgg tcggcttgcg gctcgcggtg taatgaaaga
     5401 agaagatgcg cgcatgcgat tgagcgacgc gattcaattg atcgatcgca gtcgcttcgc
     5461 tgatgtcttc cggcagcacg atcgtcggat cttccgttcg agcgggcagg gtcgctagat
     5521 aatcggtgat cagtgcatcg cccacttgac gctgatcgat caagcgctgt cgatcggcgg
     5581 ccagccgttg attcggatac aaccacgcga agttttgatt ctgaattgag atcgtcagca
     5641 caggcgcagc gtcgatccag tgctgcggca catcgggtgt gagttgaaaa tcggaagtgt
     5701 taaacaacag ataatcggcg cgcgcgaaac cggcttcgtc attcggcact aagtcgcccg
     5761 gaaaaaatgg cgcagtgccg atgatgtcac tcgctgcaat cacttttgag gcgaagggcg
     5821 aagctgctaa tgcgctgccg gctgccgcgc tttcgccgcc gccgacaggc agggcgcggc
     5881 tcgcggcatt gaatccgccc aatagcggat tgtatgctcg cagtgtcagc ggccatacgc
     5941 cgatgaattg gatcgaggtg atgataaaac aggcaacaac aaaaatgtaa aagcagcctg
     6001 agcggaacag cgaatcgaaa gatcgctgtg cagtcgaagg acgcggcgcc tgattaagat
     6061 cgcgtccttt gactacgcct cgcgcaaaga gcgaggctcc gctcaggctg ctagattctc
     6121 tcgctcgatt cttcaaccga tccgcgatta acccgatcgc gttagccagc cc
//
LOCUS       DASRTJ010000043         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_40131,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000043 DASRTJ010000000
VERSION     DASRTJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40131"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFF70_00825"
     CDS             <1..854
                     /locus_tag="VFF70_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY43265.1"
                     /translation="LKSYRIPMVKGPYTFIVTSRGCTAGCTYCIKHVSYQFSVRLRSP
                     EKIIEEIKVLNDLGITNIHMYADLFTVSREQVRGISQGLINAGMKIKWTCNSRVDYVD
                     EELLKLMARSGCWYISWGLESANEQILKHARKGTNPARAKQSLMWAKQAGIKNWGYFI
                     IGLPGETRETIQETIDYSKHLPLDIALFHIAAPYPGTPFFFEVLKNGWFRKGTQWEQV
                     DMDKSTVLDYPGLPAEVLEKEAKRAFREWAFRPGPIFTYLKSINSLSVVRSALEIGFG
                     VLGWVKG"
     gene            886..1122
                     /locus_tag="VFF70_00830"
     CDS             886..1122
                     /locus_tag="VFF70_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZY43266.1"
                     /translation="MAYQITLEIEHLEEGQYLGTSPDLPGLIVQADTPEEVIDLAPKI
                     AHQLIESLLAEGDPLPKTLKEATVPYRVQVVIPA"
     gene            1119..1325
                     /locus_tag="VFF70_00835"
     CDS             1119..1325
                     /locus_tag="VFF70_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZY43267.1"
                     /translation="MNYRELTKRLQRLGCYMTRQAAGSHEIWRNPNTQRQAVIPNPGS
                     KEIPRGTLRAILRELGIDPAEQNR"
     gene            1327..3141
                     /locus_tag="VFF70_00840"
     CDS             1327..3141
                     /locus_tag="VFF70_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43268.1"
                     /translation="MSSLAHYSSNWRQSPRRLLILSVVAIILIQTALYVQAASIRGFA
                     TGDGGVKLWQVQAILRSGSLDAPLEYPAAVYDPDHLYSPFVVPWAFWQNDQAFTEYTS
                     PFIWVSVPLFALFGQAGLLLLPWLCSTLIVIASAGLAWRVRPDRSAALVPIIVGIASP
                     ISIYGMEFWEHTPGTILAVFAIAAIVKSIDSPRRSIWLMLSGASIGLGFTMRAELYVL
                     PIAILIGLWLLRSILPFIRSIFFLSIGGLLTAGPWWLYQYLHWGSPFGPRLAQNVPLL
                     GGNDMLTRLGNTTGQNWTMLWPRAGEGLEALAVLGVIGLLLIMTLYLLNRPLTAERGL
                     RIHSILFWILIIDVLLIVVITTWRIANWQNVVDQRPDDLLSTFPVILFLPLWFGTRLA
                     FQNQQSTINNQKSEIARFLLVTPIAFVVLVLILSPFHGGVQWGPRFLLYIMPPLSVVI
                     VDRVSGWWQSVSRLRQVGLTVLFALLLIAGGFSTWMGLRFMHDSQLASAALTVAIERT
                     PEHVVVTDVWFVPQSAAYTFNDKLWLLAEDDQPMFKLIQSLRKTTNEPGMLYITALTW
                     AHLDPQTLLGARIAPNGDPQFVDSPAHYFQLSRYFLYK"
     gene            complement(3153..>3431)
                     /locus_tag="VFF70_00845"
     CDS             complement(3153..>3431)
                     /locus_tag="VFF70_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43269.1"
                     /translation="VMGAIILLIVLTIEVWARLPKVYGVYMLTIMLFSLTSGDLISMP
                     RFVVPMFPGFIALALIGKNRSPDRAILIVSILLQGVVALMFTKGYWIA"
BASE COUNT          748 a          890 c          948 g          845 t
ORIGIN      
        1 ccttgaagag ttatcgcatt ccgatggtca aaggcccgta cacatttatt gtgacgagtc
       61 gtggctgcac ggcgggctgc acgtattgca tcaagcacgt gtcgtatcaa ttcagtgtgc
      121 gcttgcgatc gccggaaaag attattgaag agatcaaggt cttgaatgat ctgggcatca
      181 ccaacattca catgtatgcg gacttgttca ccgtcagccg cgagcaagtg cggggcatct
      241 cgcaaggctt gatcaatgcg ggcatgaaga tcaagtggac gtgcaacagc cgcgtcgatt
      301 acgtggacga agaactattg aagctgatgg cgcgatcggg ctgctggtat atttcgtggg
      361 gacttgaatc ggccaacgaa caaattctga agcatgcgcg caaggggacg aatccggcgc
      421 gcgcgaaaca atcgctcatg tgggcgaagc aagcgggcat caagaattgg ggttacttca
      481 tcatcggcct gccgggtgag acccgcgaaa cgatccaaga aacgatcgat tactccaagc
      541 acctgccgct cgacattgcg ctgttccaca tcgccgcgcc gtatccgggc acgccgtttt
      601 tctttgaagt gttgaagaat ggctggttcc gtaaaggcac gcagtgggag caagtggaca
      661 tggataaatc gacggtgctg gattatccgg gtttgcccgc cgaagtattg gagaaagaag
      721 cgaagcgggc gttccgcgaa tgggctttcc ggcccggtcc gattttcact tatctcaaga
      781 gcatcaatag tttgtccgtc gtgaggtccg cgttggagat cggcttcggg gtgttgggct
      841 gggtcaaggg ctgatggaga gacagcgaga tagagggaag gcgagatggc atatcaaatc
      901 actcttgaaa tagagcatct tgaggaaggc caatatctgg gcaccagccc ggacttgcct
      961 ggcttgatcg ttcaggccga tacgccagaa gaagtgatcg atcttgcgcc aaagatcgcg
     1021 catcaattga ttgaatcatt attagctgaa ggcgatccgc tgcccaagac attgaaagaa
     1081 gctactgtac cctatcgagt gcaagtcgtt attcctgcat gaactatcgc gaactgacca
     1141 aacggttgca acgactgggt tgctatatga cgcggcaagc tgccggtagt cacgaaatct
     1201 ggcgtaaccc gaatacccag cggcaggcag ttattccgaa tcctgggagc aaagaaattc
     1261 cccgcggcac tcttcgagcg atcttgcgtg aattaggaat agatcccgct gaacagaatc
     1321 gttagcatgt ccagccttgc ccattactca tcaaattggc gccaatcgcc acgccgatta
     1381 ttaatcttat cggtcgtggc gattattttg atccaaactg cgctctatgt gcaagccgca
     1441 tccattcgcg gttttgccac cggcgatggc ggtgtcaagc tgtggcaggt gcaagccatc
     1501 ttgcgatccg gcagcctcga tgcgccgttg gagtatccag ctgcggtcta cgaccccgat
     1561 catctctact caccgtttgt cgtaccgtgg gctttttggc agaatgatca agccttcact
     1621 gaatacacct cgccgtttat ctgggttagt gtgccgttgt ttgcgttgtt cggtcaagcc
     1681 ggcttattgc tgctaccgtg gttgtgcagt acattgattg tgatcgcctc ggcggggctg
     1741 gcttggcgcg tgcggcccga tcgatccgca gcgctggtgc cgatcatcgt cggcatcgcc
     1801 agcccaattt cgatctacgg gatggaattt tgggagcaca cgcccggcac aatcctggcc
     1861 gtgtttgcca ttgcggcgat cgtcaaatcg atcgattcgc cacgccgctc gatttggttg
     1921 atgctctccg gcgcatcgat cgggttgggg tttacgatgc gtgccgaatt atatgtcctg
     1981 ccgattgcga tcctcatcgg tttgtggttg cttcgctcga tcttgccgtt catccgatca
     2041 atcttcttct tatcgatcgg cgggttactc accgccgggc cgtggtggtt gtatcaatat
     2101 ctgcattggg gtagtccgtt tgggccgcgc ttagcgcaga atgtgccgct cttgggcggc
     2161 aacgatatgc tcacgcggtt gggcaacacc accggacaga actggacaat gttgtggccg
     2221 cgcgcgggcg aaggattaga ggcattagcc gttctgggcg tcatcggctt gcttctgatt
     2281 atgacgctgt atcttctcaa cagaccgctg actgcggaac gcggattgcg gattcattcc
     2341 attctcttct ggattttgat cattgacgtc ctattgatag ttgtgatcac aacttggcga
     2401 atcgcaaact ggcagaatgt tgtcgatcag cgaccggacg atctgctcag taccttcccc
     2461 gttattttat ttctgcctct ctggtttggg acacgtctcg cctttcaaaa tcaacaatca
     2521 acaatcaaca atcagaaatc agagatcgct cgcttcttgc ttgtaacgcc catcgccttt
     2581 gttgtgctag tgttgatcct cagtccattt catggcggcg tgcaatgggg tccgcgcttc
     2641 ttgttgtata tcatgccgcc cttgagcgtc gttatcgtcg atcgagtgag tggctggtgg
     2701 caatccgtat cacgattgag gcaagttggt ctgactgtac tatttgcctt actgttaatc
     2761 gccggtggct tttcaacgtg gatgggtctg cgcttcatgc acgacagtca acttgccagc
     2821 gccgcgttga cggtggccat cgaacgcaca ccggagcatg tggtggtgac ggatgtgtgg
     2881 ttcgtcccgc aaagcgccgc ttatactttt aacgataaac tatggctgtt ggcagaagac
     2941 gatcaaccga tgtttaagtt gatccaatca ttgcgtaaaa caaccaacga gccgggcatg
     3001 ctgtatatca ccgcgttgac gtgggctcac cttgatccgc aaacgctgct gggcgcacgc
     3061 atcgcgccga atggcgatcc gcagttcgtc gattccccgg cccactattt tcagctcagc
     3121 cgctacttct tatacaagta aagacatcgg tcttacgcga tccaataccc ttttgtaaac
     3181 atcaacgcga cgaccccctg caagaggatc gagacgatca agatcgcgcg atcgggcgat
     3241 cggtttttgc cgatcagcgc taaggcgatg aagccgggga acatgggcac gacgaagcgc
     3301 ggcatcgaga tcaaatcgcc gctggtcaat gaaaagagca tgatggtcaa catgtacacg
     3361 ccgtaaactt tcggcaggcg cgcccacact tcgatcgtca gcacaatcaa taagattatc
     3421 gctcccatca c
//
LOCUS       DASRTJ010000044         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_78072,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000044 DASRTJ010000000
VERSION     DASRTJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78072"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..707)
                     /locus_tag="VFF70_00850"
     CDS             complement(144..707)
                     /locus_tag="VFF70_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43270.1"
                     /translation="MSDGKSLQDEGRKLYHGGQYEEAAAKLVEAQQAFVAANDQANAA
                     ECANDRGVCWRQAAKWAEATAAFAEARSMFQALNDAKGEGQVVGNIAALADSQNDKDH
                     AADLYLEAIDLLEKAGAKDLAKDTYTALSRLCLKRGNFMGAISAYDSGLEEGDKPNMM
                     QRVVRRILGGSTKPPKATEKKDELNSS"
     gene            complement(718..1401)
                     /locus_tag="VFF70_00855"
     CDS             complement(718..1401)
                     /locus_tag="VFF70_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZY43271.1"
                     /translation="MSTVVVPETIAIRRRIISVLLQGARLKTGKSKKECADAIGATTG
                     AYAAYEAGRRDISLPELEVLAHYLKLPISDFFENQDRVVNEEPEVNRSQVMVLRQRII
                     GALLRKARTDKGKSAKDLAELLGLPPRRIAQYELGQRPIPLAQLQEMADALQMSMRYF
                     IDEGFGTIGEQEQTRTQFELFNDLPDDLRKFVSSPSNTSYLRVAQHLSGLTTEQLRNV
                     AASILDITY"
     gene            1677..2888
                     /locus_tag="VFF70_00860"
     CDS             1677..2888
                     /locus_tag="VFF70_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZY43272.1"
                     /translation="MVNDFPGKGTVAVLKTQPKTVLDDYRKLLNLAGYQQALPKDRGT
                     LLKINISWQTWYPACSTAPWQLEGVIKAMQADGYNLIGAHNDTVVVDTADGEANNKQK
                     FVTDKYGVPCVYLYKPDVEWIEYKPKTPFLVLDKVYPEGLFIPKTFYGKNIVHLPTVK
                     THVFTTITGAMKNAFGGLLHRKRHWTHGVIHETLVDLLQIQQDIHSGIFAVMDGTFAG
                     DGPGPRAMHWHEKDIILASSDQVAIDAISAKLQGFDPMSIQFLRMAHNRGLGIADPRQ
                     IKIVGYDIEQEAPWNFVQTDTFASKGQKMIYHGPLKPLEGLLLRSPIVPWSFFASNFY
                     HNVYWYPFVGRPRVEAALQTKWGQLFKEYGDGQVVMPGVEPKTIRQAAMGAAAVGAAM
                     LALPLMFRRRK"
     gene            2945..>3130
                     /locus_tag="VFF70_00865"
     CDS             2945..>3130
                     /locus_tag="VFF70_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43273.1"
                     /translation="MAKIHGIGRMTIGEMREAVKQGARFVVFEYVLSFIVISRRRVSA
                     TFFVRPGESTFSMSLPYN"
BASE COUNT          709 a          865 c          813 g          743 t
ORIGIN      
        1 cagaaggaac atccgcactt gcggttagcg gaatctgacc acgcgtaaaa aagttcggac
       61 agcagttgaa ctgctgtccg aacaaatagc ccaaaacaaa actgcgagac ttaacccctc
      121 gcagttttta ttttattcaa aaattaagaa gaattcaatt cgtctttttt ctctgtggcc
      181 ttcggtggtt tggtcgatcc gccgagaatt ctgcgaacga cgcgctgcat catattcggc
      241 ttatcgccct cttctaaccc tgagtcgtac gcgctgatcg cgcccatgaa attgccgcgc
      301 ttcaagcaca agcgactgag cgcagtgtaa gtgtctttag ccaaatcttt tgcgcctgct
      361 ttttccaaga ggtcgatcgc ttcaagatac aaatccgccg catggtcctt atcgttctgc
      421 gaatcagcca acgcggctat attgcccacg acttgtcctt cgcccttagc atcgttcaag
      481 gcttgaaaca tcgatcgggc ttcagcaaag gccgcggtcg cttctgccca tttcgccgct
      541 tgccgccagc atacgccgcg atcgttggcg cattcggcgg cgttggcttg atcgttcgcc
      601 gcaacaaacg cctgctgggc ttcgacaagc ttcgccgcgg cttcttcata ctggccgccg
      661 tgatataatt tccgtccttc gtcttgcaat gatttcccgt cgctcatatc acaaccttta
      721 ataggtgatg tccaagatcg atgcggcgac gttgcgcagt tgttccgtcg tcaggccgga
      781 caagtgctgc gctacgcgta aataactggt attggatgga cttgacacaa acttgcgaag
      841 gtcatcgggc aaatcattga agagctcaaa ttgagtgcga gtctgttctt gctcgccaat
      901 cgtgccgaaa ccttcatcga tgaaatacct catggacatc tgcagcgcgt cggccatctc
      961 ttgcagctgc gccaatggaa ttggccgctg accaagttca tactgcgcga tacgacgcgg
     1021 tggaaggcct aagagctcgg ccaggtcctt ggccgatttt cctttatcgg tacgcgcttt
     1081 gcgcagcagc gcgccgatga tgcgctgtcg caaaaccatc acctggctgc ggttcacttc
     1141 tggttcttca ttgacaacgc gatcttgatt ctcaaaaaaa tcgctgatgg gcagcttcag
     1201 gtaatgcgcc aggacttcca gttccggcaa ggaaatatca cggcggccgg cttcatacgc
     1261 ggcgtacgcg cccgtcgtcg caccgatagc atcggcacac tcttttttcg atttgcccgt
     1321 tttgaggcgc gcaccttgca gcaaaaccga aatgattctg cgtcgaatcg cgatggtctc
     1381 tggcacaacc acagtcgaca tgccgatccc tccggcggtg atttaataac tcgatcggtg
     1441 atcacatcga tcgagaaaac cgatcgattt cgcgcattat accatagaga cagatcgcaa
     1501 gtggctaatt gcaaatcgcg catcgtgtat cccatatcgc cccttgtcgt ctgccgtttg
     1561 catccgcgaa atgcccctgt tgcgaagcgg agtgggattg ccccgttcga tcatcgcttg
     1621 tcatgacttc attgctcagg tacaataaaa gccacatatt ttttggagga tgcaatatgg
     1681 taaatgattt ccccggtaaa ggcaccgtcg cagttttgaa gacgcagccg aagacggtgc
     1741 tcgatgatta ccgcaaacta ttgaacttgg cgggctatca acaagccctg cccaaagatc
     1801 ggggtacgct gcttaagatc aacatctcgt ggcagacgtg gtatcccgct tgctcgactg
     1861 cgccgtggca attggaaggc gtcatcaagg cgatgcaggc cgatggctat aacttgatcg
     1921 gcgcgcacaa tgatactgtc gtcgtcgata cggctgatgg cgaagcgaac aacaagcaga
     1981 aattcgttac cgacaaatac ggagtgccgt gcgtttatct ctataagcca gacgtcgaat
     2041 ggatcgagta caagccgaag acaccgttcc tcgtgctcga taaagtttat cccgaagggt
     2101 tgttcattcc caaaactttc tacggcaaga acatcgtaca cctgcccacg gtcaagacgc
     2161 atgtcttcac gacgatcacc ggcgcgatga agaacgcgtt cggcggcttg ctccatcgca
     2221 agcggcattg gacacatggt gtcattcacg agacgctggt cgatctgctt caaattcaac
     2281 aagacattca ctccggcatc ttcgccgtga tggatggcac gttcgccgga gacggccccg
     2341 gcccgcgtgc gatgcactgg cacgagaaag acatcatcct ggcttcgagc gatcaagtgg
     2401 cgattgatgc gatctccgct aagctgcaag gcttcgatcc gatgtcgatc cagtttctgc
     2461 gcatggcgca caatcgcggc ctcggcattg ccgatccacg ccagatcaag atcgtcggtt
     2521 acgacattga acaagaagca ccgtggaact tcgtccagac cgataccttc gcgagcaaag
     2581 gccagaagat gatctatcat ggcccattga aaccgttaga agggttgttg ttgcgctcgc
     2641 cgatcgtgcc gtggagtttc ttcgcgagta acttctatca caacgtgtac tggtatccct
     2701 tcgtcggccg gccacgcgtc gaggccgcat tgcaaacgaa gtggggccaa ttgttcaaag
     2761 aatacggtga cggacaagtg gtcatgcccg gcgtggaacc gaagaccatc cgccaagcag
     2821 cgatgggcgc agccgccgtc ggtgcggcga tgctggcgct gccgttgatg tttcgacgac
     2881 gcaaataatt tttcagatac gtaggggcga gtttgtaact cgcccctcct cttgtgtgaa
     2941 gagtatggca aagattcacg gcatcggtcg catgacgatt ggtgaaatgc gcgaagcagt
     3001 taaacaaggc gcgcgtttcg tcgtcttcga atatgtgctg tcgttcatcg tcatctctcg
     3061 acggcgagtg agcgccacgt tcttcgtgcg gcccggcgaa tcgacgttct cgatgagcct
     3121 gccgtataac
//
LOCUS       DASRTJ010000045         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_152489,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000045 DASRTJ010000000
VERSION     DASRTJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152489"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFF70_00870"
     CDS             complement(<1..191)
                     /locus_tag="VFF70_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY43274.1"
                     /translation="MEVAPNVHQITLNFVNAFLIIDEDGLTLIDTGLPGSDKKIIEYL
                     SGIGRLPNNLKRIILTHSD"
     gene            296..1801
                     /gene="malQ"
                     /locus_tag="VFF70_00875"
     CDS             296..1801
                     /gene="malQ"
                     /locus_tag="VFF70_00875"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZY43275.1"
                     /translation="MTFDRASGVLLHPTSFPGPDGIGDLGPEAYRWIDFLSSAGCALW
                     QVLPLGPTGYGDSPYQCFSAFAGNPYLISPDLLLKENLLVTTDIADRPQFPIDRVDFG
                     PAITWKLTLLDRAYRRFRIDRSKQLREEFALFQIDQSSWLDDFSIFMALKEAHQSAWG
                     DWPKALRQRDPQALQAACIQHADSIEKHAFRQFLFFRQWSALRTYAHTKKIKIVGDIP
                     IFVAYDSADVWSQPELFYLNSRGQPTVVAGVPPDYFAPTGQLWGNPLYRWAVHQRSGY
                     AWWLERLRATFRQVDIARLDHFRGFAGYWGVPADQLTAEKGKWTTGPGKIFFKAIQRA
                     LGELPIIAEDLGEITPDVIELRDAFDLPGMKILQFAFNGASPLNPFLPHRFPVNCVVY
                     TGTHDNDTIRGWYASASEREREFALKYLNTAEGNLSVAMIRAAWSSVANMALASMQDL
                     LDLDGSARMNFPGRESGNWGWRMRADALTFELRDRVKELNILYGRVNPAPG"
     gene            1822..2763
                     /locus_tag="VFF70_00880"
     CDS             1822..2763
                     /locus_tag="VFF70_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43276.1"
                     /translation="MAIDPLVTVIIATYNSRGTLALTLNSVLNQDWTDFEVWIVGDEC
                     TDNSAEVVSAFNDPRLNWVNLPKNTGSQSEPNNEGLRRARGQYIAYLGHDDLWLPWHL
                     STLIAHLQATSADLVHSLCVMIAPAGVFDAIGLPRDGVNYDLHTIVPSTWLHRLEVAG
                     AIGGWRDPHSISRAADFDFTRRAFVAGRRIEFAPVLSVLKFGSAWWKSYVRTGDPPQA
                     AYLQALLKDPIAVRDQVLFDMAVAYTRYYRSVGKVSVRINLREVYRDLHRLAGSIGRA
                     AIDKFGRERFPLKRLLIVLQSRNVRKLRRARGLPPLK"
     gene            complement(2787..3152)
                     /locus_tag="VFF70_00885"
     CDS             complement(2787..3152)
                     /locus_tag="VFF70_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HZY43277.1"
                     /translation="MSAYKPLHFIHESIKVIFDRPQTYEKKPDCPNGFIWNDKTYRVT
                     KNLEEWHDYARKGRMAANMQPVHAEAAKTKGSWGVGRYYFRVRVNAGQIFEIYYDRAP
                     KGSKHRKGAWFLIGELIEA"
     gene            complement(3194..>3779)
                     /locus_tag="VFF70_00890"
     CDS             complement(3194..>3779)
                     /locus_tag="VFF70_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZY43278.1"
                     /translation="QWAEEIRQTQTPRSTWSGRWPSLATQFVVGAVGESDLEILTTSA
                     YLIQKLHLARTYFSAFNPVSDTPLENHPAENPVREHRLYQTSFLLRDYGFDLEDMPFT
                     QNGFLPLDVDPKIAWAKMNLSETPLEINRADRRELLHVPGIGPKGVEAILKARRKGKL
                     LDLKDLKSIGVIASRAAPFLLLDGKRPMRQLSLW"
BASE COUNT          800 a          947 c         1061 g          971 t
ORIGIN      
        1 atgtcggaat gagtcaggat gatccgtttg agattgttgg gtaatcggcc gatcccgctc
       61 agatactcaa taatcttctt gtcactgccc ggcaagccgg tgtcaatcaa cgtcaagccg
      121 tcctcatcga taatgagaaa ggcattcacg aaattcaatg tgatttgatg aacgtttggt
      181 gcgacttcca tgctgtaccc cctttcgcaa attgtttgca gagattgtat catcacttgg
      241 aaagaaatga ggcctgcgct atacttgccc cttgatctga gcttggagaa aaataatgac
      301 gttcgatcgt gcttcaggtg tgttgctgca tccgacgagt ttccccggcc ccgacggtat
      361 cggcgatctt gggccagagg cttatcgctg gatcgatttt ctatcgtcgg ctggttgtgc
      421 gttgtggcag gtattgccgc tcggtcccac cgggtatggc gattcgccgt atcaatgttt
      481 ctcggccttt gcgggtaacc catatttgat cagtcccgat cttttgctta aagaaaactt
      541 attggtcaca actgatattg ctgatcggcc gcagttcccg atcgatcgag tggattttgg
      601 cccggcgatc acctggaagt tgacgttgct cgatcgggca tatcggcgat ttcgaataga
      661 tagatcgaag cagctgcgag aagagttcgc cttattccaa atcgatcaat ctagctggct
      721 cgacgatttt tcaatcttca tggccttgaa agaagcgcat caatccgcct ggggcgattg
      781 gccgaaggcg ctgcgccaac gcgatccaca agcgctgcaa gccgcgtgca ttcagcacgc
      841 agactcgatc gagaaacatg cctttcgcca attcttgttc ttccgccagt ggtcagcctt
      901 gcgcacgtat gctcacacaa aaaagatcaa gatcgtcggt gatataccga tcttcgtcgc
      961 gtatgattcg gcggatgtat ggtcgcagcc cgaattattc tatcttaata gtcggggaca
     1021 accgaccgtc gtagcgggtg tgccgcccga ttattttgcg ccgaccggac agttatgggg
     1081 caatccgtta tatcgttggg ccgtgcatca acggtcgggt tatgcctggt ggctggaaag
     1141 attgcgcgct acgtttcggc aagtggatat cgcgcggctc gatcactttc gcggctttgc
     1201 cggttattgg ggagtgccgg cggatcagct caccgctgaa aaaggcaaat ggacaaccgg
     1261 cccgggcaaa atttttttca aagccattca gcgcgcgctg ggtgagctgc cgattattgc
     1321 tgaagacctc ggcgagatca cgccggatgt gattgaactg cgtgatgctt tcgatttgcc
     1381 cggcatgaag attttgcaat tcgctttcaa tggtgcctcg ccgctaaatc catttttgcc
     1441 gcatcgtttt ccggtgaact gcgtggtgta tactggcacg catgacaacg atacgatccg
     1501 cggttggtat gccagtgcct ctgagcgcga gcgcgagttt gccttgaagt atctcaacac
     1561 ggctgaaggc aatctgtcgg tagcgatgat tcgcgccgcg tggagttcgg tggcgaatat
     1621 ggcgttggct tccatgcaag acttgctcga tctggatgga tcggcgcgca tgaatttccc
     1681 cggacgcgaa tcgggtaact ggggctggcg tatgcgcgcg gatgcattga cgttcgaact
     1741 gcgcgatcga gtgaaagaac tgaatatctt gtatgggcgc gtcaatcctg ctccaggata
     1801 agcggcaggc acaatcatcc aatggcaata gatccgttag tcacggtcat cattgcaact
     1861 tacaatagca ggggaacgct ggcgctgacg ctgaacagtg tgttgaatca ggattggact
     1921 gactttgagg tatggatcgt cggtgatgaa tgtaccgaca acagcgctga agtggtgtct
     1981 gcgtttaatg acccgcgctt gaattgggtc aatcttccca agaacaccgg cagtcaaagc
     2041 gaaccgaata atgaagggct gcgtcgagca cgcggccaat atattgctta tctcggtcat
     2101 gatgatttat ggctgccgtg gcatctctca acattgatcg cacacttgca agcgacatct
     2161 gccgatctgg tgcatagctt gtgtgtaatg atcgcgccag cgggtgtgtt tgacgcgatc
     2221 gggttgccgc gagatggcgt gaattacgac ctgcatacga ttgtgccatc gacgtggctg
     2281 catcgcttgg aagtggccgg agcgatcggc ggttggcggg atccgcacag tatctcacgc
     2341 gcggcagatt ttgattttac gcggcgagca tttgtcgctg gccggcgcat tgaatttgcg
     2401 cctgtattga gtgtgttgaa atttggctcg gcctggtgga aatcgtatgt tcgaacgggc
     2461 gatccgccgc aggccgcgta tttgcaagcg ctgcttaaag atccgatcgc cgttcgtgat
     2521 caagttttat tcgatatggc cgttgcctat acgcgttatt atcgatcggt gggcaaagtc
     2581 agtgtgcgca tcaatttgcg cgaggtctat cgggacctgc atcgcttggc tgggtcgatc
     2641 ggccgggcgg caatcgataa gtttgggcgt gagcgttttc cgctcaagcg attgttgatc
     2701 gtgctccaaa gccgtaatgt ccggaagctg cgtcgagcgc gtggtctacc gccgctaaaa
     2761 taacgcgacg acccggctgt tcgtctctat gcttcgatca attctccaat taaaaaccat
     2821 gcgcctttgc gatgtttcga gcctttgggc gcacgatcat aatagatttc aaaaatctgt
     2881 cccgcgttta ctcttactcg aaagtaatag cggccaaccc cccacgagcc tttcgtcttg
     2941 gcggcttcgg catgtaccgg ttgcatgtta gcggccatgc gtcccttgcg ggcgtaatcg
     3001 tgccactctt ctaagttttt cgtgactcga taagtcttat cgttccagat gaagccgttg
     3061 ggacagtctg gtttcttttc gtaagtttgt ggccgatcaa agataacctt gatcgattcg
     3121 tggatgaagt gcagaggctt gtatgcggac atatcataac ctgacaggct gtaataactt
     3181 gtcagattta agattaccac aaactcaatt gtcgcatcgg ccgtttgccg tccagcagca
     3241 aaaacggcgc ggcgcgcgac gcgatgacgc cgatcgattt caagtctttc agatcaagta
     3301 gcttgccttt tcgccgcgcc ttcaagatcg cttcgacgcc tttcgggccg atgccgggca
     3361 cgtgcagcag ctcgcgccga tcggcgcggt tgatctccag tggcgtttca ctgagattca
     3421 tcttcgccca cgcaatcttc ggatcgacat cgagcggcaa gaagccattt tgcgtaaagg
     3481 gcatatcctc tagatcgaaa ccataatcgc gcagcaaaaa tgaagtctgg taaagtcggt
     3541 gctcgcgcac cggattctct gccggatgat tttcgagcgg cgtgtcactg accggattga
     3601 acgcggagaa gtacgttcgg gcgagatgca atttctgaat gagataagcc gaggtggtga
     3661 gaatttccaa atcgctttcg ccgactgcgc cgaccacgaa ctgcgtggcg agtgatggcc
     3721 agcgcccgct ccaggttgat cgcggagttt gcgtctggcg aatctcttcc gcccattgc
//
LOCUS       DASRTJ010000046         3607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_2631,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000046 DASRTJ010000000
VERSION     DASRTJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFF70_00895"
     CDS             <1..358
                     /locus_tag="VFF70_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43279.1"
                     /translation="SRIMRPQGVALPMMNMATRLSVANDRITKIAIAAAPVAPTPFRC
                     TQTESFLKGKPANSDSIELAIEVLLSECKPRTSAHRATAEYRREVLPVLLRRTVGKAI
                     DRAKTGEVVPEIYKVE"
     gene            531..3329
                     /locus_tag="VFF70_00900"
     CDS             531..3329
                     /locus_tag="VFF70_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZY43280.1"
                     /translation="MPILNFTLNNKPVSVEAQPNESLAEVLRYRLGMTGTKIGCNEAE
                     CGICTVIVDGQPIDSCIYPALKADGAAIETIEGLANGHLHPLQENFIKFGAVQCGFCT
                     PGLIMQSKALLDRKAGEQVSEEEIKIALKDTYCRCTGYVSVLKAIKAANGQNVSVEDY
                     LPETKHGYNVVGEALPRADSIAKVTGAAIYTDDVSFPGMLFGATLRAMIPHALIKKID
                     TRKAKALPGVHAVLTHEDVPGNKNHGLVTLDWPVLCYDKVRYVGDAVAIVAADSIEIA
                     KAALQLIDVEYEPLPIVATAEYAHQADSPLVHDSGNLLKHIKVAKGDVDQGFAEADVV
                     IERTYHTACTEHAFLEPECSIGRITDDGRYEIYVGSQIVYQDRDQVAAVLGIAEDQVR
                     IISTLIGGGFGGKEDIAGQIHVALLAKATRRPVKMLYTRHESLIFHPKRHATTITVKL
                     GAKKDGRLTAAKAELYGDTGAYASLGEKVLTRATTHANGPYAVANAKSDCFAMYTNNV
                     PAGAFRGFGVTQSAYAVEMTIDELAEQLQIDPIEIRKMNALKVGSTTNTGQVLRESVG
                     LIECIEKVVDEINRTTAPSTSSSSHFVRSESALDASLPANKKRAWGIAVGYKNTGLGG
                     GANDCAGAEIEVYPSGISEIRTSSAEIGQGLPGVLAAIVAEELGLPIEKVNVLLSDTD
                     RTPNGGPTTASRQTFVSGNAARHAAIQMRANMSSVAAERLDVSPDSLIFRNSRVEHNG
                     KSASFAEVVEWMQAEGRSTKLTYEYTAPTTQPLGTGGDMHFAFSYAAHAALVEVDTDT
                     GEVKVLKVIAAHDIGRAINPLALEGQIDGGIVMGIGNALTEEFPHEKGIPYVQWLARY
                     KMPSIKHTPEIKSFIVEHEASTGPFGAKGVGEISSIPITPAITNAIYNAVGVRVRRLP
                     VDQDTLLRAMKSVANSVD"
     gene            complement(3420..>3607)
                     /locus_tag="VFF70_00905"
     CDS             complement(3420..>3607)
                     /locus_tag="VFF70_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43281.1"
                     /translation="GTISSVPDTTTINRLTRDQYATHSIGPQATTDLTGTNGTLVAVD
                     LLLSLPYYATDLPLVTK"
BASE COUNT          879 a          930 c         1056 g          742 t
ORIGIN      
        1 ctcgcgcatc atgcgcccgc aaggcgtggc actgccgatg atgaacatgg ccactcgctt
       61 gagcgtagcc aatgatcgga ttaccaagat cgccatcgcc gctgcgcccg ttgcgccaac
      121 cccatttcgt tgtacacaaa cagaatcgtt cttaaaaggc aaacccgcga attcagattc
      181 gatcgaatta gcgattgaag tcctgttgag tgaatgcaag ccgcgcacca gcgcccatcg
      241 cgccacggcg gaatatcgac gcgaggtgct gcccgtttta ttacgacgca cagtgggcaa
      301 ggcgatcgat cgagcgaaga ccggcgaagt tgtaccggag atctataaag tggaatagcg
      361 agtaggcgag gcaactgcta aatttgtcat ttcgagcgta gcgagaaatc tctttcacaa
      421 taatgatatg acgctgatgg gcatagagat ttctcgtcgc tccgctcctc gaaatgacaa
      481 caaccaacac cttactcatt actcgttatt caatagtttg aggagacacc atgccaatat
      541 taaactttac tctcaacaat aaacctgtgt ccgtcgaagc acagccaaat gaatccctgg
      601 ccgaagtgct gcgctatcga ttggggatga ccggcacgaa gatcggctgc aatgaagcgg
      661 agtgcggcat ctgcaccgtc atcgtcgatg gacagccgat cgattcgtgc atctatcccg
      721 cgttgaaagc ggacggcgca gcgatcgaaa cgatcgaagg tttagcgaac ggacatctgc
      781 atccgctaca agaaaacttc atcaagttcg gcgcggtgca gtgcggtttc tgcacgccgg
      841 gtttgatcat gcaatcgaaa gcgctgctcg atcgcaaggc aggcgagcaa gttagcgagg
      901 aagagatcaa gatcgcgttg aaggacacct actgtcgatg tacaggctat gtgagtgtgc
      961 tgaaggccat caaggccgcg aatggacaaa atgtttcggt tgaagactat ttaccggaaa
     1021 caaagcacgg ctataacgtc gtcggcgaag cgctgccgcg cgccgattcg atcgcgaaag
     1081 tgaccggcgc ggcgatttac accgatgacg tttcattccc cggcatgtta ttcggcgcga
     1141 cattgcgcgc gatgattcca cacgcgctga tcaaaaagat cgatacgcgc aaagcgaagg
     1201 ccttgcccgg cgttcacgcg gtgttgactc atgaagatgt gcccggcaac aagaatcacg
     1261 gtctagtcac tctcgattgg ccggtgctgt gctatgacaa agttcgatat gtcggtgatg
     1321 cagtcgcgat cgtcgccgcg gattcgatcg agattgcgaa ggccgcattg cagctgatcg
     1381 acgtagagta tgagccgtta ccgatcgtcg ctacagccga atacgcacat caagccgatt
     1441 cgccgcttgt tcacgatagc ggcaacttat tgaagcatat caaagttgcg aagggcgatg
     1501 tcgatcaagg ctttgccgaa gctgatgtgg tgatcgagcg aacatatcac actgcttgta
     1561 ccgagcacgc gttccttgag ccggaatgtt cgatcgggcg gatcacggac gacgggcgct
     1621 acgaaatcta cgtcggctcg cagatcgtct atcaggatcg cgatcaagtt gcggcggtat
     1681 tgggcatcgc tgaagatcaa gtgaggatca tcagcacttt gatcggtggc ggattcggcg
     1741 gcaaagaaga catcgccgga caaattcatg tggcgttatt ggcgaaagcg actaggcgtc
     1801 cggtcaagat gttgtacacg cgccatgagt cgttgatctt ccatcccaag cgacacgcga
     1861 cgacgatcac ggtcaaactc ggcgcgaaga aagacggacg actgaccgca gcgaaagccg
     1921 aattatatgg cgatacgggc gcgtatgcgt cgctcggtga aaaagttttg actcgcgcga
     1981 cgacgcacgc gaacgggccg tatgctgtgg cgaatgcgaa gtcggattgt tttgcgatgt
     2041 acacgaacaa tgtgcccgcc ggtgcgttcc gcggattcgg cgtgacgcaa tcagcttacg
     2101 cggttgagat gacgatcgat gaattggctg agcagctgca gatcgatccg atcgagatcc
     2161 ggaagatgaa cgccttgaaa gttggatcga cgacgaatac gggacaggtc ttgcgtgaga
     2221 gcgtgggctt gatcgaatgt attgagaagg tggtggatga gatcaatcgc acgaccgctc
     2281 cttcgacttc ttcttcgtcg cacttcgtgc ggtcagaatc cgctctggat gcctcattgc
     2341 ccgccaataa gaaacgcgcg tggggcattg cagttggcta caagaacact ggacttggcg
     2401 gcggcgcgaa tgattgcgcg ggcgcggaga tcgaagtgta tccatcgggc atcagcgaga
     2461 ttcgcacttc gtcggctgag atcggtcaag gcttgcccgg cgtgctggcc gcgatcgtcg
     2521 ccgaagagtt gggactgccg atcgagaaag tgaatgtgct gttaagcgat accgatcgca
     2581 cacccaacgg cggaccgaca accgcctcgc gccaaacatt cgtcagcggc aatgccgcgc
     2641 gacacgccgc gatccaaatg cgcgcaaaca tgtcgagcgt ggcggctgaa cgcttggacg
     2701 tctcgcccga ttcattgatc ttccgcaata gtcgagttga gcacaatggc aaatcagcct
     2761 cgttcgcgga agtcgtcgaa tggatgcaag ccgaaggtcg atcaacaaaa ctcacttacg
     2821 aatacactgc gccgacgacg cagccgctcg gcacgggcgg cgatatgcac tttgcgtttt
     2881 cgtatgcggc gcatgcggcc ttggtcgaag tcgataccga tacgggcgaa gtgaaagtgt
     2941 tgaaagtgat cgccgcgcac gacatcggcc gcgcgatcaa tccgctggcg ctcgaaggcc
     3001 agatcgacgg cggcattgtg atgggcatcg gcaacgcgtt gaccgaagag ttcccgcacg
     3061 aaaaaggaat tccgtatgtg cagtggctcg cgcgctacaa gatgccgagc atcaaacaca
     3121 caccggagat caaatcgttc atcgtcgaac acgaagcctc gaccgggccg ttcggcgcga
     3181 agggcgtcgg cgaaatcagc agtatcccga tcacgcccgc gatcacgaat gcgatttata
     3241 atgcggtcgg tgtgcgtgtt cgcagactac cggtcgatca ggatacgttg ctgcgggcga
     3301 tgaagagcgt cgcaaacagc gtggattgat taatccgcta aaaagcaggg cataaactac
     3361 tggtgataaa acatttaaga cctgatagat tcactcacga aaatctgcca ggtctttttt
     3421 tattttgtga ccagcggcag atcagtcgcg tagtagggta gactcagcaa aagatcgacg
     3481 gcaaccagcg tgccattggt gccagtgaga tccgtcgtgg cttgcggacc gatcgagtgg
     3541 gtggcatatt gatcgcgggt caggcgattg atcgtagttg tatcgggcac tgagctaatc
     3601 gtgccga
//
LOCUS       DASRTJ010000047         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_305332,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000047 DASRTJ010000000
VERSION     DASRTJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305332"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VFF70_00910"
     CDS             <1..1521
                     /locus_tag="VFF70_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZY43282.1"
                     /translation="FTPAERELMLDGINEQLSDLEKIRSVSISNTVPSPLHFDPRLPS
                     MQFDHQKYRSKFSRPRAPQVPENLEELAFCSIGELAALIRSCKVTSLALTRMYLDRLK
                     RHDPVLKCVVTLTEGLAIKQARRADREIAVGQYRGLLHGIPYGAKDLLAVSGYKTTWG
                     SETHQNQIIDLDATVIDRLEEAGAVLMAKLSVGALAWGDVWFGGMTKNPWNTEEGSSG
                     SSAGSASAVAAGLVGFAIGTETLGSIVSPATQCRVTGLRPTFGRVSRHGAMALCWSMD
                     KIGPIARTVEDCAIVFDAIYGSDGLDQDVVDMPFNYDATLNVKKLRVGYVKSAFDKER
                     DNQANDDATLKVLKSIGVKLIPIELPELPINAMGIILQAEASAAFDELTRSNRDDLLA
                     RQVKDAWPNVWRHSRLIPAVEYVQANRVRYLAMQALAKLMDKIDVYVCPSFGADNLTL
                     TNLTGHPCVVVPNGVDAKGKSSSISFTGKLYGEAAALSLAKAYQLKTDFHLQHPKL"
     gene            1620..1991
                     /locus_tag="VFF70_00915"
     CDS             1620..1991
                     /locus_tag="VFF70_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZY43283.1"
                     /translation="MDKTFFFIGSLSGAIGVLLGAFGSHGLRKRLTPESMATFEIGVR
                     YQMYHAFALIAVPIANGQGRASSLPSISGWLFAIGTILFSGSLYLLALTQKRWLGAIT
                     PFGGLAFIAGWVCLALSALSG"
     gene            2004..2963
                     /locus_tag="VFF70_00920"
     CDS             2004..2963
                     /locus_tag="VFF70_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43284.1"
                     /translation="MDLFLATAKGLVIAQRDDDVWRVKSRSLDGKELTCVASSGSTIY
                     VGALDGVYRSTDRGQTWIELKEGLSIKHARWIAYHADRVFVGTEPANIFVLHEGESMW
                     HECTEVAKLRDVHQWFLPYSPEAGCIRGFAFHGSRAYAAAEVGGSLRSNNHGETWALC
                     GGSTGNPSLDDPPAPYIYPDVHSVEVHPSSADLLYAPTGGGFYRSMDGGQTWKSFYDC
                     YVRAAWIDPIDPNHIILGPADGVSSNGRIEESRDGGKSWKKASNGLDVPWRRYMVERF
                     VQVGDDLLAVLSNGQLLSAALTSLKWQRILVDVPNVNGLAALS"
     gene            3030..>4299
                     /locus_tag="VFF70_00925"
     CDS             3030..>4299
                     /locus_tag="VFF70_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43285.1"
                     /translation="MLGLHFAILHYASARSSTLPSQPTNLTVNYRSGQSFITWTERAD
                     LVSETYRIYRYSQAITAANLMSAGLLYDVPEGTARFFENRFWTGSVWKNRYLDRYVIQ
                     DHGPQLITGTGLLIWTFSPQDFNGGNSGNAYYAVTTVYSGTENTIDFNSANSIGPIGE
                     VITDPLPVEISNTVGAGWHVFIQYMDLRNWNPTFHAPNGTNSYYGLNSADPEVARALQ
                     YAYDYAIFQPGATQCGSMLPAQLPASVWLHPWQGNNYPPASGSSTWCAYQISPIDETE
                     TWWFGFARDHDFRKGGSISHTDRIVNYTEQRVLRMIYDLERSPIGPPVDPNRVYVSGY
                     SMGAGGTLGFALRYPNVFAAAYATKPATNYQLAGGWVGNISAKWGSTAMSVPVLINAP
                     NHWADGLQSHTGESVWDWENHQSNLMNRQTD"
BASE COUNT          948 a         1043 c         1239 g         1069 t
ORIGIN      
        1 ttcacccctg ccgaacgcga gttgatgctc gacgggatca atgagcagtt gagcgatttg
       61 gagaaaatcc gatcggtctc gattagcaac actgtcccat cgccgctgca tttcgatccg
      121 cgtctgccca gcatgcagtt cgatcaccag aaataccgat cgaagttttc tcgtccacgc
      181 gcaccgcaag tgccggagaa tttagaggaa ctagctttct gctcgatcgg tgaactggcg
      241 gcgttgattc ggtcgtgcaa agtgacttcg ctggcattga cgcggatgta tctcgatcga
      301 ttgaagcgcc acgatccagt gttgaaatgc gtggtgacct tgactgaagg cctcgccatc
      361 aaacaagccc gccgcgccga tcgagaaatt gccgtgggtc agtatcgcgg tctgctgcat
      421 ggcattccct acggcgcaaa agatttgttg gcggtcagcg gttacaaaac gacgtgggga
      481 tcagaaacgc accagaatca aatcattgat ctcgacgcca cggtgatcga tcgcctggaa
      541 gaagcgggcg ctgttttgat ggcgaagctg agtgtcggcg cgttggcttg gggcgacgtg
      601 tggttcggcg gcatgactaa gaatccctgg aacaccgaag aaggctcgag tggctcgtcg
      661 gccggatcgg cctcggcggt tgccgcagga ctcgtcggct ttgcgatcgg cactgagacg
      721 ctgggatcga tcgtgtcgcc cgcgacgcag tgtcgcgtaa ctggcttgcg tccaactttc
      781 ggccgggtca gtcggcacgg cgcaatggcc ttgtgttgga gcatggataa gatcggaccg
      841 atcgcacgca cggttgaaga ctgcgcgatt gtgttcgatg cgatctatgg atcggatgga
      901 ctcgatcaag atgtggttga tatgccgttc aactatgatg cgactctgaa tgtcaagaaa
      961 cttcgcgtgg gttacgtgaa aagcgcgttc gataaagagc gcgataatca agcgaatgat
     1021 gacgcgacgt taaaggttct gaaatcgatc ggggtgaagc tgatcccgat cgagttgccg
     1081 gaattgccga tcaatgccat gggcatcatt ctgcaagccg aagcttcggc ggcgttcgat
     1141 gaactcactc gatcgaatcg cgatgattta ttagcgcggc aagtaaaaga tgcctggccg
     1201 aatgtatggc ggcattcacg cttgattccg gccgtcgaat atgtccaggc gaatcgcgtt
     1261 cgatatctgg cgatgcaggc gctggcgaag ctaatggaca agatcgatgt gtatgtctgt
     1321 ccgtcattcg gtgcggacaa tctaacgctg accaatctga ccggccatcc atgcgttgtc
     1381 gtgccaaacg gcgtcgatgc gaaaggcaaa tcgagcagca tttcatttac cggaaaattg
     1441 tatggcgagg cggcggcttt atcgctggcg aaggcatatc aattgaaaac cgattttcat
     1501 ttgcaacatc caaagttata accacctgtt attgcgagtg atgcgaagca atctcaaatt
     1561 cgattgggag attgcttcgt cgaaaaaacg ctcctcgcaa tgacgtatag aacctaagta
     1621 tggataaaac atttttcttc atcggtagtc tctctggtgc gattggcgta ctgttgggcg
     1681 cattcggatc acacggcttg cgtaagcggc tgacacctga atcgatggcg acgttcgaga
     1741 tcggcgtgcg gtatcagatg tatcatgcct tcgcgctgat tgccgtgccg atcgcaaatg
     1801 ggcaggggcg cgcatcgagc ctgccgagta tttccggttg gttgtttgcg atcgggacga
     1861 ttttattttc aggcagcctg tatctcttgg cgttgactca aaaacgttgg ctcggtgcga
     1921 tcacgccatt cggcggattg gcctttatcg cgggttgggt gtgcttggct ttgtccgcat
     1981 tgagtggctg aaggagatca caagtggatc tatttctcgc aactgcaaag ggtctggtta
     2041 tcgctcaacg cgatgatgac gtttggcgcg ttaaatcgag gtcacttgat ggcaaagagt
     2101 tgacttgcgt ggcttcgagc ggcagcacga tctatgttgg cgcgctggat ggagtttatc
     2161 gatcgaccga tcgcggccaa acatggatcg agttgaagga aggcttgtcg atcaaacacg
     2221 cacgctggat cgcgtatcac gccgatcgag tttttgttgg gacagaaccg gcgaatattt
     2281 ttgttttgca cgaaggcgaa tcgatgtggc atgaatgcac tgaagttgca aaattacgtg
     2341 atgtgcacca gtggttcctg ccttattcgc cagaggctgg ctgcatccgc ggtttcgcat
     2401 ttcatggctc gcgagcgtac gctgctgccg aagtcggcgg ttcattgcga tcgaataatc
     2461 acggcgagac ttgggcatta tgcggtggca gcaccggcaa tccgtcgctc gatgatccac
     2521 ctgcgccata tatttatcct gacgtccatt cggtcgaagt ccatccatca tcagccgatc
     2581 tcctttatgc gccaactggc ggtggctttt atcgatcgat ggatgggggt cagacatgga
     2641 aatcttttta cgattgctat gtgcgcgccg cgtggatcga tccgatcgat cccaatcaca
     2701 tcatccttgg cccggccgat ggggtcagct cgaacggtcg gattgaagaa tcgcgcgacg
     2761 gcggaaagtc gtggaagaaa gcatcgaatg gtttagatgt cccgtggcgg cgatacatgg
     2821 tcgaacgttt tgtgcaggtc ggtgatgatc tgctggccgt tttatcgaat gggcaactgc
     2881 tgagcgcagc attgacttcg ttgaagtggc agaggatttt agtggatgtg ccgaatgtca
     2941 acgggctggc tgccctaagt taaggattaa tattgaagca gaggttgctt tatcgaggca
     3001 tagagtcatt aatcttaagc cttaccattt tgctgggact gcatttcgcg atcttgcatt
     3061 acgcctcggc tcggtcttcg acgttacctt ctcaacctac aaacctcacc gtcaattatc
     3121 gcagcggcca gtcattcatt acctggaccg agcgcgcgga tctggtcagt gagacctatc
     3181 ggatctatcg ttactcgcaa gcaatcacgg cagccaatct gatgagtgcg ggtttgttgt
     3241 atgatgtgcc agagggcact gcacgtttct tcgagaatcg tttttggacc ggcagtgtgt
     3301 ggaagaatag atatctcgat cgatatgtta ttcaagatca cggcccgcag ttgattacag
     3361 gcacgggact tttgatttgg acattctcgc cgcaagattt caatgggggt aacagcggca
     3421 atgcgtatta tgcggtcacg actgtctaca gtggaacgga aaacacgatc gatttcaaca
     3481 gcgctaattc gatcggtccg atcggcgaag tgatcaccga tccgctgcca gtggaaatca
     3541 gtaatacggt tggcgctggc tggcatgtgt tcatccaata catggatctc cgcaattgga
     3601 atccaacttt tcatgcgccg aatggcacca atagttatta cggcttgaat tccgccgatc
     3661 ccgaagttgc tcgcgcgctt caatatgcgt atgattacgc gatttttcag ccgggcgcaa
     3721 cccaatgtgg tagcatgctg cccgcgcagc ttccggcatc agtttggctg catccttggc
     3781 agggcaacaa ctatccgccg gcctctggct cctccacttg gtgtgcttac caaatcagcc
     3841 cgatcgatga gactgaaaca tggtggttcg gcttcgcgcg tgatcacgat tttcgcaagg
     3901 gtggctcgat cagccatacc gatcggatag tcaactatac cgagcagcgt gtgctccgga
     3961 tgatatatga tttggagcgc agcccgatcg gcccgccggt cgatcccaat cgcgtgtatg
     4021 tcagtggtta ctcgatgggt gcaggcggca ccttgggttt tgctctacgc tatcccaatg
     4081 tttttgccgc ggcttatgcg actaaacccg ctacgaacta tcagctggct ggaggctggg
     4141 tcggcaacat ttcagcgaaa tggggttcga ccgcaatgag tgtgccggtg ttgatcaatg
     4201 cgccaaatca ttgggcggat ggattgcaat cacacacggg tgaatccgtt tgggattggg
     4261 aaaatcatca gagtaatttg atgaatcgcc agacagatc
//
LOCUS       DASRTJ010000048         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_341870,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000048 DASRTJ010000000
VERSION     DASRTJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341870"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1034)
                     /locus_tag="VFF70_00930"
     CDS             complement(192..1034)
                     /locus_tag="VFF70_00930"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43286.1"
                     /translation="MTTRRSPWLKLFSFLVYGFLYAPILILILFAFNSSRINVIFEGV
                     VNQGPCGPFYWFCVLSQNEEVIAAAGNTLKIALASTVVSTIIGTLAALALQRYKFRLK
                     SFSEISLYIPIVIPEIVMGISLLVLFSGIFTWLNSALGLTGDAQLHLGLVTVILAHIA
                     FSVPFVVLVVRARLHSFDKSVEEAAMDLGANEWQTFRRVTLPLIMPGVLSGAMLAFTL
                     SLDDFIITFFTNGPGSTTLPIYVYGLLRRIVTPEVNALSTVWIAVVFAVLLLSQLVQR
                     RSNA"
     gene            complement(1031..1954)
                     /locus_tag="VFF70_00935"
     CDS             complement(1031..1954)
                     /locus_tag="VFF70_00935"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43287.1"
                     /translation="MLKRLREQRSLQGTLLISPALLWMIVLLIIPLILIVFTSFGQRD
                     PDGNPKFDQLTAGNYVRLTGYTEGCRDFGEYLSGLWQTIIGQNTTASTAERCFDPLYV
                     TILWRSIVLAFETTLVIIVLGYPLAYYVARAAPRRRNFLLFLILIPLWTNFVIRVYAW
                     IRILRAFDILYTPGAVLIGMVYEFLPFMILPMYTSLEKIEPSLYEAAADLGASWIKTF
                     FRVTLPLSLPGVIAGTILVFVPVMGTFVVSDILGGKQFVLVGNLIQSQFLSARDSTFG
                     SAASIILMLMTLVITFLYTRKFGFGEEITAA"
     gene            complement(1961..3064)
                     /locus_tag="VFF70_00940"
     CDS             complement(1961..3064)
                     /locus_tag="VFF70_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43288.1"
                     /translation="MAEFAVELRDIVKEFDDPGRSADKVLAVNHVTLQIQDGEFFSLL
                     GPSGCGKTTSLRMIAGFELPTSGEIYLHGKPMGRIPPFQRAVNTVFQNYALFPHMTIG
                     ENVAFGLQMKGVPKADTGPRVKEALEMVRLPGYENRKPKQMSGGQQQRVALARALVNR
                     PEVLLLDEPLGALDLKLRKEMQLELKTLQREVGITFIYVTHDQEEALTMSDRIAVMSK
                     GVALQVGNSADIYERPNSRFVADFIGETSFMRGQIESQQNRSVMVALDGGLSVRASVD
                     TTLATGREVSVAVRPEKMHINAKLTLDNQLRGHVVSVVYIGTDTHYGVNLSNGQFVRV
                     REQNNTPDSRPVAHEGDEVTVSFAADAARILTE"
     gene            complement(3142..3264)
                     /locus_tag="VFF70_00945"
     CDS             complement(3142..3264)
                     /locus_tag="VFF70_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43289.1"
                     /translation="MRLDRRQRALINADRSAARLDRPTVGPGRSIELQLRRVVL"
     gene            3263..>3648
                     /locus_tag="VFF70_00950"
     CDS             3263..>3648
                     /locus_tag="VFF70_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZY43290.1"
                     /translation="MLQSPDFTRSLPLNYHMIRKISGVVICACVLAACANTLTASSSK
                     LRVVATTTIVGDITRQIAGDQIDVITLLAPGIDPHDYEPVPIDLQVISQAKLILANGA
                     GLEPWLNKITDSTASTDKVVYVSKGLT"
BASE COUNT          914 a         1028 c          963 g          743 t
ORIGIN      
        1 gggcggcaca gcagttggcg ctaaggtcgg cacaggcgtt gctgccggag tgcaggcgga
       61 caacaataag ccgatcgcga taagcgccgc catccctttc ttaagtgtgt gacttgcttt
      121 gaacattttt tcctccaaaa attttataga gatggctgat gacgaatggc tcagccatcg
      181 acttgctaca cttatgcgtt gctgcgacgt tggactaatt gcgagagcaa caagaccgcg
      241 aacaccacgg caatccaaac ggttgataac gcgttgactt ccggcgtaac gatgcgccgt
      301 agtaaaccgt aaacgtagat cggcagcgtc gtcgagccgg gcccattggt aaagaaggtg
      361 atgatgaaat catccagcga tagcgtgaaa gccagcatcg cgcccgataa gacgccgggc
      421 atgatcagcg gcagtgtcac gcgccggaaa gtctgccatt cattcgcgcc caaatccatg
      481 gcggcttctt ccacgctctt atcgaagctg tgcagacgcg ctcgaacgac gagcacgacg
      541 aacggaacgc tgaaagcgat atgtgccagg ataacggtga ctaaccccag gtgcaactgc
      601 gcatcgccag tgagtcccaa agcggaattc agccaggtga atatcccgct gaacaaaacc
      661 agaaggctga tacccatcac gatctcggga atcacaatcg ggatgtacag tgatatttcc
      721 gaaaatgatt ttaatcggaa tttgtagcgc tgcaaagcca gcgcggccag ggtaccaatg
      781 atcgtcgaga cgaccgtcga cgccagcgca atcttcaacg tgttgccggc tgcggcaatc
      841 acctcttcgt tctgggatag cacgcagaac caatagaacg gcccgcacgg cccttgattg
      901 accacgccct caaagatcac attgatgcgc gaagagttga aggcgaaaag gatcaggatc
      961 agaatcggcg catacagaaa gccatagacc aaaaaagaaa agagcttcag ccagggcgag
     1021 cgtcgagtag tcatgcggcc gtgatctcct cgccgaagcc gaatttgcgg gtgtacaaga
     1081 acgtgatcac taacgtcatt aacatcaaga tgatcgaggc cgccgagccg aaggtcgagt
     1141 cacgcgcgct gaggaactgg ctttggatca aattgccaac cagcacaaat tgtttgccgc
     1201 ccaaaatatc ggagacgaca aacgtgccca tcaccggcac aaagaccaag atcgttccgg
     1261 cgatcacacc gggcagcgat aagggcagcg tcacgcgaaa gaaagttttg atccacgacg
     1321 cgcccagatc agcggcggct tcatacagcg aaggttcgat cttctccagc gaagtgtaca
     1381 tgggcaaaat catgaacggc aaaaactcat acaccatgcc gatcaacacc gcgcccggtg
     1441 tgtacaaaat atcgaaggca cgcaggatgc gaatccaagc gtacacccga atgacgaagt
     1501 tggtccagag cggaatcaga atcaggaaca ataagaaatt acgccgccgg ggcgctgcac
     1561 gcgccacata gtaggctaag ggataaccca gcacaatgat gaccaacgtc gtttcaaacg
     1621 ccagcacaat cgagcgccac aagatggtca catagagcgg atcgaaacag cgctcggctg
     1681 tactggccgt ggtgttctgc ccgataatcg tttgccacag cccgcttaag tactcaccaa
     1741 aatcgcggca gccctcagtg tagccggtca accgcacata gttacccgca gtcagctgat
     1801 cgaatttggg gttgccatcg ggatcgcgct ggccgaagga agtaaacaca atcaaaatca
     1861 gcggaatgat caacaacacg atcatccaca acagcgccgg tgagatgagc agtgtgcctt
     1921 gcagcgatcg ctgttcgcgc agacgtttga gcataatggc ctactcggtc aggatgcgcg
     1981 ccgcatccgc cgcaaaactt accgtcactt catcgccttc atgcgccacc gggcgcgagt
     2041 cgggcgtatt attttgttcg cggactcgca caaattggcc gttggataaa ttgacgccgt
     2101 aatgcgtatc cgtgccgatg tacacgacac tgacgacatg gccgcgcagt tgattatcaa
     2161 gtgtcagctt cgcattgatg tgcattttct ccggccggac ggccacactt acttcccgcc
     2221 cagtcgccaa cgtcgtatcc acgctggccc gcacggacaa accgccgtcg agcgccacca
     2281 taaccgatcg gttttgctgc gattcaatct ggccgcgcat aaaactcgtt tcgccgatga
     2341 agtctgctac gaatcgggaa ttgggccgtt cataaatatc ggcactgttg cccacttgca
     2401 gcgccacgcc tttgctcatg accgcgatgc gatcggacat ggtcagcgct tcttcttgat
     2461 cgtgtgtcac atagatgaag gtaatgccga cttcacgctg caatgttttc aattcaagtt
     2521 gcatttcttt gcggagtttg agatcgagtg cgccgagggg ttcatccaac aacagcactt
     2581 cgggtcgatt cactaaggcg cgggccagcg ccacgcgctg ctgctgccca cccgacattt
     2641 gtttcggctt gcgattttcg tatccgggca ggcgcaccat ttccagcgct tctttaacgc
     2701 gcggcccagt gtcggctttt ggcacgcctt tcatctgcag accgaaggct acgttctcgc
     2761 cgatggtcat atgggggaac aaggcatagt tttgaaaaac ggtgttgacc gcgcgctgaa
     2821 agggcggtat gcggcccatg ggtttgccat gcaaataaat ttcgccggag gtgggtagtt
     2881 caaagccggc gatcatgcgc agcgaagttg ttttaccgca gcctgagggt cccaacagcg
     2941 agaagaattc gccatcctgt atttgcagcg tcacatgatt gacggctaat accttgtcag
     3001 cactgcgccc cggatcgtca aactccttca cgatatcgcg cagctcaacc gcaaactccg
     3061 ccatacagcc tccacaccct gtcgatattt ctctagccga tgtcgactca gttcgagttg
     3121 atcgaagcct aatttgtccg attatagcac gacgcgtctg agctgcaact cgatcgatct
     3181 tcccggccca acggttggcc gatcgagccg agcggctgat cggtcggcgt tgatcaacgc
     3241 gcgttgacgt cgatctagcc gcatgctaca atcgccagac tttacgcgca gtttgccgct
     3301 aaactatcac atgatcagaa aaatatctgg agttgtcatc tgcgcgtgtg ttctcgcggc
     3361 ttgcgcgaac acactgacag cttcatcaag caaactacgc gtcgttgcca ccacaaccat
     3421 cgtcggcgat atcacgcgcc agatcgctgg cgaccagatc gatgtgatca cgttgctggc
     3481 acccggcatc gatccgcacg actatgagcc ggtgccgatc gatctgcaag tgatcagcca
     3541 ggccaaactt attctggcga acggcgcggg cttagagccg tggctgaaca aaataacaga
     3601 cagcaccgcc tcaacagaca aagtggtcta tgtgtcaaag ggcttaac
//
LOCUS       DASRTJ010000049         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_114765,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000049 DASRTJ010000000
VERSION     DASRTJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114765"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFF70_00955"
     CDS             complement(<1..222)
                     /locus_tag="VFF70_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HZY43291.1"
                     /translation="MKHRLIFSTVLAVTFVFATFVSLQSKSVRAASLIIVDTAADETT
                     VNGQCSLREAIIAANRDIAVDSCTAGSGID"
     gene            complement(342..1661)
                     /locus_tag="VFF70_00960"
     CDS             complement(342..1661)
                     /locus_tag="VFF70_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZY43292.1"
                     /translation="METLSLIFIAIIGTILASVLALIPALHIYNVVGLLILSAGALSS
                     VLNPDQLAMLLLGMIVGYAMLSTIPSIFVAAPDEAAAFIVLPGQKWLLERRGYEAAIL
                     TGVGALCGLAVLIVISPVAAEATRVLQIILSPHLGWILATIIVFMLMSEWPKSGDRGS
                     SGWARFRAAWTGLGAGLLTFLLSGLLGIIIMYRSVVPTEIAFQNLLPAFLGLYAIPMV
                     LTNLIMGTQVPKQHIASSIDITPGLLFRGAAAGSMGGLFAAFFPVVTGGIGGFLAGHA
                     TAQRDERSFIVSQGTSKFVYYVGGFLLFFLPGLHRAKGGMAQMLSTIYTPATPELYFT
                     VIAAMAICGAIAFFLLILFSRLAIKLIDRIDYRVVSIVTLILLITLIFLTTGLGGLLI
                     ALPATAIGLIPSLFGSRRMNCLGILLIPITLNMAGVGASIAHVLGLI"
     gene            complement(1729..>3676)
                     /locus_tag="VFF70_00965"
     CDS             complement(1729..>3676)
                     /locus_tag="VFF70_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZY43293.1"
                     /translation="YVFATHAPIAQVKIRDVESSFNYAYVQIDGQVSRGPSYNADSKS
                     LTFYVRDDTGELMVSAFRSTADELIAADKIPAPGDTVSVQGTLRVREAVPSLNVESPQ
                     ALTLQRVTENPPTRDIGSITSVDALHGVTLRGMVRSIKIPYDGLKLVTLRDATGSIDV
                     AISSDMESLLGAAPEITVAQSIQVIGSVTLFDTTPQLTLNRGSDMQGLDQAIEIAPPV
                     SIGDLRDSDAGRFVKVSGEVEIVSPFSAGVKVSLIQNDQHMSVLFWQDFWDDLSISSQ
                     ITFGAQLSVQGELNVFRGKLEIVPEIDQDVALTSFAPVNPTVTPTPTSTIEIISKPIG
                     QITDKDVDSIVLTSGSIEKVDDFSQGTRYTLKDNTGSITLLMWNDRIDPARQRELFQA
                     GVTLSVTGKIDQFNGQLEIVPDSVDQVTVISASSNPPSASTPAATPEVRSTSTPAPTA
                     APVSSAPAASIGQLSQDNAGQTYQVQATVVEVSSFSAGFDFLLDDGTGRIKLTLFNDT
                     YKFVPGRSALNLGAEISVAAKVTVFNGALELQPQSGKDVMILKPGSNGSVAVRQVNSL
                     GKPGEIVAIEGTLTDIKDFSSGKNVFVDDGTGNVQVTIFNNVLAYVPVSKLIVGASVR
                     VVGKTDFFGRIQVVPQLGYDVTIK"
BASE COUNT          890 a         1041 c         1011 g          734 t
ORIGIN      
        1 atcgatgccg ctgcccgccg tgcagctgtc gacagcgata tcacggtttg cggcgatgat
       61 cgcttcgcgc agcgaacatt ggccattgac ggtggtctca tcggcagccg tgtcaacgat
      121 gatcaggctg gccgctcgga cggatttgct ttgcagcgaa acgaaagtag cgaagacaaa
      181 cgttactgcg agtacagtgg agaagatcaa tcggtgtttc attgttgcct cctaagctca
      241 atgcagaaac gaaaacaaaa ggaaagataa agggggaacg tcgtgcgtag tgctgatcaa
      301 tctctatcat acacctgtag tcaagcgcgg agcattgatg cttaaatcaa tcccagcaca
      361 tgcgcgatcg atgcgccgac accggccata ttcaacgtga tcgggatcaa gaggatgccc
      421 aaacagttca tgcggcgcga tccgaacagc gaggggatca aaccgatcgc cgtcgcgggc
      481 agcgcgatca ataaaccgcc gaggccagtc gtgagaaaga tcaacgtgat caacaaaatc
      541 aaagtcacaa tactaacgac tcggtaatcg attcgatcga tgagcttgat cgccaatcgg
      601 ctgaacagga ttagcaggaa gaaggcgatg gcgccgcaga tcgccatcgc cgcaatgacg
      661 gtgaagtaca actctggtgt cgccggcgta tagatcgtgc tcaacatctg agccatgccg
      721 cccttggcgc gatgaaggcc cggcaggaaa aacaacaaga agccgccgac gtaatagaca
      781 aatttcgaag tgccttgcga tacgatgaag gatcgttcgt cgcgctgcgc tgtggcgtgt
      841 ccggccagga agccgccgat gccgcccgtc acgaccggaa agaaggctgc gaacaaaccg
      901 cccatcgatc cggccgccgc gccgcgaaac aataacccgg gcgtgatgtc gatcgagctg
      961 gcgatgtgct gtttgggcac ttgcgtgccc atgatcaaat tcgtcagcac catcggaatg
     1021 gcatacaggc cgagaaaggc gggtaataaa ttttgaaacg cgatctcggt cgggacgacg
     1081 ctgcgataca tgatgatgat gcccagcagt ccggagagca ggaaagtgag aaggccggcg
     1141 ccgagtcccg tccacgccgc gcgaaacctc gcccaacccg acgaaccacg atcgccgctc
     1201 ttgggccatt cactcatcaa catgaagacg atgatcgtgg cgaggatcca gccgagatgc
     1261 ggcgataaaa taatttgcag cacgcgcgtg gcttcagccg cgactggact gatgacgatc
     1321 aacacggcga ggccgcacaa tgcgccgaca ccggttaaga tcgccgcttc gtagccgcgc
     1381 cgctcgagca gccatttttg tccgggcaag acaatgaagg ccgcggcttc atcgggtgcc
     1441 gcgacaaaga tcgacgggat ggtgctgagc atcgcgtaac caacgatcat acccagtaag
     1501 agcatcgcca gctgatcagg attcaagacg ctcgatagcg cgcccgccga caagatcagc
     1561 aagccgacca cattgtagat gtgcagcgcg ggaatcaacg ccagcactga agccagaatc
     1621 gttccgataa tagcaatgaa aatcaacgag agagtttcca taggcgaagc gtccaatcta
     1681 acgagtcagc atttcactcg tcactcatta ctcgtcactc gttactcttc atttaatcgt
     1741 cacatcgtaa ccaagctgcg gcaccacttg tatccggcca aagaaatccg tcttgcccac
     1801 tacgcgcacg cttgcgccga cgatcaattt cgaaacgggc acataggcca gcacgttgtt
     1861 gaagatcgtc acctgcacat tgcccgtgcc gtcgtcgacg aacacatttt tgccggatga
     1921 aaaatctttg atgtcggtaa gcgtgccttc gatggcgacg atctcgccgg gcttgcccag
     1981 cgagttgacc tggcgcacgg cgacactgcc gttcgatccc ggcttgagga tcatcacatc
     2041 tttgcccgat tgcggctgca gttccaacgc gccgttgaag accgtcactt ttgccgccac
     2101 ggaaatttcc gcgcccagat tcaaggccga tcggcccggc acaaatttat aggtatcgtt
     2161 gaacaacgtc aatttgatcc gtcctgtccc atcgtccaac aagaaatcaa aaccggcgga
     2221 aaaactgctg acttcgacga cggtggcctg cacttgataa gtttgtccgg cgttgtcctg
     2281 cgacagttga ccgatcgatg cggctggcgc ggaagaaacc ggcgcagcgg ttggcgcggg
     2341 cgtggaagtg gatcgaactt cgggtgtggc cgcgggcgtt gaagccgaag gtggattgct
     2401 cgacgctgag atcaccgtga cttgatccac agagtccggc acaatttcaa gctgaccgtt
     2461 gaactgatcg atcttgccgg tcaccgataa agtgacaccc gcttggaata gttcgcgctg
     2521 cctggctgga tcgatccgat cgttccacat caataatgtg atcgatccgg tgttgtcttt
     2581 caacgtgtag cgtgtgcctt gactgaagtc atctactttc tcgatcgatc cactggtcaa
     2641 tacgatcgag tccacatctt tgtcggtgat ctggccgatc ggtttggaga tgatctcgat
     2701 cgtggacgtg ggtgtcggcg taacggttgg attcactggc gcaaaactcg tcagcgcgac
     2761 atcttgatcg atctcaggca caatctccaa cttgccgcga aaaacattca gttcgccttg
     2821 cacactgagc tgcgcgccga atgtgatctg cgaggaaatc gataggtcat cccaaaaatc
     2881 ctgccagaac aaaacgctca tatgctgatc gttttgaatg agcgaaactt tcacgcccgc
     2941 cgagaacggc gaaactattt cgacctcgcc gctgactttc acgaagcggc ccgcgtccga
     3001 atcgcgcaga tcgccgatcg agacgggcgg cgcaatctcg atcgcttgat caagcccctg
     3061 catatccgag ccgcgattca aggtgagctg cggcgtcgta tcgaacaacg tcacggagcc
     3121 aatcacttga atggactgag cgaccgtgat ctcaggcgcg gcacccaata aactttccat
     3181 atccgacgag attgccacat cgatcgagcc ggtggcatca cgcagcgtga ctaacttcaa
     3241 gccgtcgtat ggaatcttga tcgatcgcac catgccgcgc agcgtcacgc cgtgcagcgc
     3301 atccacgctg gtgatcgatc cgatgtcgcg cgtcggcgga ttttcggtga cgcgctgcag
     3361 cgtcaacgct tgcggcgatt caacatttaa tgaaggcaca gcctcgcgca cacgcagcgt
     3421 gccttgcacc gagaccgtgt cgccgggcgc gggaatctta tcggcggcga tcagctcatc
     3481 ggcggtagat cgaaacgcgc tgaccatcaa ctcgcccgtg tcatcgcgca cataaaacgt
     3541 caacgatttt gaatcggcgt tgtagcttgg cccgcgcgaa acttgtccat cgatctgcac
     3601 ataggcgtag ttgaacgacg attcgacatc acgaattttt acctgcgcaa tcggcgcgtg
     3661 cgtggcaaac acatac
//
LOCUS       DASRTJ010000050         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_305734,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000050 DASRTJ010000000
VERSION     DASRTJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305734"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            883..1893
                     /gene="trpS"
                     /locus_tag="VFF70_00970"
     CDS             883..1893
                     /gene="trpS"
                     /locus_tag="VFF70_00970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZY43294.1"
                     /translation="MKKGRILTGDRPTGPLHLGHYVGTLANRVKLQDDYEVFLLVADY
                     HALTRNVEREHVAAIQANRRLLVLDYLAVGIDPDKTTIYVQSDVPSVAELHLLFSMLT
                     TVPRLERVPTLKEIMEAEHIEQPSYGLLGYPVLQAADILHVRANLVPVGRDQASHVEV
                     TREVARRFNFLYGEVFPEPDTLIGEVPTLVGTDNKAKMSKSLGNAIMLSDDAETVRQK
                     VMNMYTDPARVRMDIPGNPDGNPVFQYHTAFNPNTEEVEDLKSRYRAGKVGDVEVKKK
                     LNAALQNFLEPIRERRAKYAGQPKLIDEILDAGAAKVRPISAETLRMARDAMGFAHKT
                     TE"
     gene            2163..2627
                     /locus_tag="VFF70_00975"
     CDS             2163..2627
                     /locus_tag="VFF70_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZY43295.1"
                     /translation="MKLSKRGEYGLKAMIELAVRESERGVVQIKTLATHEQIPVKFLE
                     QIMVTLKHAGLLQSKAGLGGGYHLARPAREITLGQIVRVLDGPLAPIRCVSQMAYARC
                     VCEDEKTCGLRLVMLDVRNAIADTLDSATLADVAARQRRAESRARRIVRRAT"
BASE COUNT          664 a          639 c          769 g          644 t
ORIGIN      
        1 tcgtatggca atgcaacagg gatacaccga gatggtgccc catacggtag tcatccagcc
       61 caatcagaag agcagaaaat tccagtgtcg tccgtgacat tcgtgttact gcggctgagt
      121 aaggtgtaac attagccgtg cggattatca cagggattcg caataacttc tggagcgact
      181 aacagcgcag gaaactcatc agactagtat aagtccttca ctcccacacc ttttgtggct
      241 aaggttaagc catcacgaca aaggcggcgc gagtcgaatt gaatagttga acctgaaggc
      301 tgctcctccc acaagatcga gcagccttct ttgatcgatc gaagggccag atcgatcgat
      361 cggattcatg ttggccgcgc agctgtgttc gatcaaaatc attgtagact ttagctcaac
      421 ccgatcgtta aaactggcct caagaatcgt gcttgacagc cgcggcgaaa ttgtggtaga
      481 ctccgcctca cacagacgtt gatgaggaat agtacacatc aatcaacaca cagagagtcg
      541 gatagtaagg ggtgagagtc cgatgcgagc gttgatgtga aatggtcttc ggagtcgcga
      601 gagcgaaatc gatcgagagt agctcaagcc ggaaacgcca ccgttatcaa ggcagaaggt
      661 atcagggtat ggtacctgca agagtgaggc tggctaaacc tgtcgcaatc accgacaggt
      721 ttttctattg ctagtctaaa ctgggtggca ccacgggcac attttttacg ctcgtcccat
      781 gcgcgtatcg gcgcgtgtgc gatgggcgtt tttgtttgac tcaggaccat tgtgtgtaga
      841 tagtttcaaa gaaaatcagt cgatgacaca aggagtcgtg atatgaaaaa aggacgaatt
      901 cttaccggcg atcggccaac cgggccgctg catttaggtc actacgttgg gacgttagct
      961 aatcgagtga agctgcaaga cgattacgaa gtgttcttgc tcgtcgccga ttatcacgcc
     1021 ttgacgcgca atgtcgagcg tgagcatgtg gccgcgattc aagctaaccg gcggttactg
     1081 gtgctggatt acttagcggt cggtatagat cccgataaga cgacgatcta cgttcaatct
     1141 gatgtgccga gcgttgccga gctgcatttg ttgttctcaa tgctgacgac ggtgccgcgg
     1201 cttgagcgtg tgcccacgct taaagaaatc atggaggcgg aacatatcga gcagccatcg
     1261 tatggtttgt tgggttatcc cgttttgcag gctgctgaca ttttgcacgt ccgcgcgaat
     1321 ttggtgccgg tcggcagaga tcaggcttcg cacgttgaag tgacgcgtga agtcgcgcgc
     1381 cgtttcaatt ttctatacgg cgaagttttt cctgagccgg atacgcttat cggcgaagta
     1441 ccgacgctcg tgggcacaga caacaaagcc aagatgtcaa aatcgctggg caatgcgatc
     1501 atgttatcag acgatgcgga gacagtaaga cagaaggtga tgaacatgta taccgatcct
     1561 gcgcgcgtcc gcatggatat ccccggcaat ccggacggta atcctgtctt tcaataccac
     1621 actgcgttca atccaaatac agaagaagtt gaggatctga agtcgcgtta tcgcgccggc
     1681 aaagtcggcg atgtggaagt caagaagaag ttaaacgcgg cgttgcaaaa ttttctggaa
     1741 ccgatccgcg agcgccgcgc caaatatgca ggccagccga aattgatcga tgagattctc
     1801 gatgcgggcg cagctaaggt tcgaccaatt tcagcggaga cactgcgcat ggcgcgtgac
     1861 gcgatgggtt ttgcgcataa gactaccgag tagatcgggc agctcaatgc ctgtgtatga
     1921 ttatcctcgc gtagaaaatg gacaggcgat tgcggacttg ccaagggtta cagccacttt
     1981 agctgattgg cgcgaaccag ttttacttcc tatgcagaca ccgatcgatc cgatcggcgt
     2041 ctgttattat cgctgacggt catcagcgat acgggatcag gtgtttgaca acctggcaat
     2101 ctcttggtaa cctgtaatta ttctaaaccc tattatatta gtagactatt gggtgattga
     2161 ccatgaaact ttcgaagcgc ggcgaatatg gattgaaggc tatgatcgaa ctggccgtgc
     2221 gcgagtctga acggggcgta gttcagatca aaacgcttgc gacccatgag cagatccccg
     2281 tcaagtttct ggagcagatc atggttacac tgaagcatgc gggtttgctc cagagtaagg
     2341 caggcctagg tggcggctat cacttggcac gacccgccag ggagattacg ctgggccaga
     2401 ttgtgcgcgt gctggacggt ccgttggctc cgatccggtg cgtcagccag atggcttatg
     2461 cgcgctgtgt gtgcgaggat gaaaagactt gcgggctgcg gttggtgatg ctggatgtgc
     2521 gcaacgcaat cgccgatacc ttggatagtg ctactttagc tgatgtggcg gcgcgccaga
     2581 ggcgcgcgga gtcacgtgcg cgccgcatag ttcgccgcgc aacttaatta aattattgca
     2641 taactgttcg gctgcaggcg aggagaggct cgatttatct tcccgctatt ttaatgatgt
     2701 aaatatctag taatac
//
LOCUS       DASRTJ010000051         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_41035,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000051 DASRTJ010000000
VERSION     DASRTJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41035"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFF70_00980"
     CDS             <1..455
                     /locus_tag="VFF70_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43296.1"
                     /translation="WLVLIIFLVVAIAIIGRIQYGLSQAQAREREAVFMYELSTALAG
                     LNQQADIARMLAKQLQQIYQAALVQVILQADLESRSIMASAPPSAIAQGKPDRTVPIM
                     AARGMTGEICLWRGEIPLPSIDNRLLQNFATQGALALERARLVHGGNQ"
     gene            601..3306
                     /locus_tag="VFF70_00985"
     CDS             601..3306
                     /locus_tag="VFF70_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZY43297.1"
                     /translation="MSDQRPDPDELLARVKHEESKRLRGRLKIFFGATAGVGKTYAML
                     EQARAMKAKGVDVLAGVVETHGRAETEAMLQGLDILPPRLVDYRGTQLQEFDLDAALH
                     RHPALILLDELAHTNATGSRHAKRWQDVDDLLTVGINVYTTMNVQHMESLNDVVAQIT
                     NVVVRETVPDSIVEQADEVELIDLPPDELIQRLREGKVYVSQQAEQAIENFFRKGNLI
                     ALRELALRRTADRVDAQMQMYRRDHSIATTWPTNERLLVCIGPNPLATRLVRAARRMA
                     AGLHAEWLVVYVETPGHLRLPEADRNRVIDTLRLAERLGAETITLSGQTVSEEIINYA
                     RRRNISKIVVGKPHRSPWRQWLRGSIVDAIIRSSGEIDVYVITGDRGEPRSIQRAIGL
                     RRTSSWSAYAWGMVIVLGCTGLAGLLQPLLDPLNLVMIYLLGVVLTAVRYGRGPSILA
                     TILSVAAFDFFFVPPFLTFAVSDTQYIITFGVMLVTALIISNLTWRTRQQAEAARHRE
                     RRTAALYAMGRELVSMRGIQNLASAALRHVNEVFNCQVIILLPDANGEMTSPVLPTNF
                     AIDVKEQSVAQWVFDRSQMAGKDTDTLPSATALYLPMIASRGTIGVLGVRTIDKLNLS
                     EPEQLHTLEAFASQAALAIERARLSDEAEQAKIQVETERLRNSLLSSVSHDLRTPLAS
                     ISGAASSLLEADLLDAETRRDLAEAIYEEASRLDRLVRNLLDMTRLESGAIKLQKEWQ
                     PLEEVIGVVLARLADQLKDRSVSTHLPIDLPLVPLDGVLIEQVLLNLLENAIKYTPAG
                     SPIDLSAWADHDQVTIEIADRGPGLPVGEEQRIFEKFYRAQKPETHGVGLGLTICRGI
                     VEAHGGRIWAENRSGGGTTFRFTLPLEGTPPKIDSNIKSIAAESS"
     gene            3303..4010
                     /locus_tag="VFF70_00990"
     CDS             3303..4010
                     /locus_tag="VFF70_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43298.1"
                     /translation="MITSALGDLVLVIEDEPQIRRFLRATLVSHGYRFAEAATAQLGL
                     MQAATYNPQVIILDLGLPDFDGLEVTRRLREWTTVPIIVLSARGQEQDKIAALDAGAD
                     DYLTKPFGVGELVARIRVALRHAARAADPAHQSSFTAGNIHVDLDQRRIFVDKAEVHL
                     TPIEYSLLTTLIRYAGKVVTHHQLLKEVWGEAYADESHYLRVYMGQLRRKLEADSTRP
                     RYLLTEPGVGYRLVMDE"
     gene            4200..>5281
                     /locus_tag="VFF70_00995"
     CDS             4200..>5281
                     /locus_tag="VFF70_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43299.1"
                     /translation="MLFSIILLVVVCFLAYKVSPRENKEHAHVHGAGQIALLAALALF
                     GVDYFTSYFYATGEMMSALHPYGLQNYGYIAVLVIGLANFVFGGLYIYSLGVFNEGGG
                     SYTASMRYLAPTLSLIVAVTLIEDYVLTIVVSALSGGDQLLSALGAYGTTWIWHFVIG
                     ASLAAVTWYLTIRGRGESSRVVFTLLGIFGLLTLIMAIGLIVANVKGVPALPADQATE
                     AASLGQAILHLLTASMKGMVALTGLEAMSNGIQFVINEDAGIVKWGKQHLPKFKKLWN
                     FYSGKSGIGRLVQSSFLFYGGLTTLFLTFFAIRFNVFDGTAGRTLVGNLAFIGFTQIP
                     GGEILFWAYQLLAVALLAAASMTAFQ"
BASE COUNT         1126 a         1410 c         1493 g         1252 t
ORIGIN      
        1 gttggttggt gctgatcatt ttcttagtcg ttgccattgc catcatcggc cgcattcaat
       61 acggtttgtc gcaggcgcaa gcccgcgagc gtgaagcggt gttcatgtat gaactcagca
      121 cggccttagc cggcttaaat caacaagccg atattgcgcg catgctggct aaacaactcc
      181 agcagattta tcaagcagcc ttagtgcaag tgatcctgca agccgatttg gagtctcgat
      241 cgatcatggc gagcgcaccg cccagcgcga tcgcgcaggg caagcccgat cggacggtgc
      301 cgatcatggc ggcgcgcggc atgaccggcg agatttgttt gtggcgcggc gaaattccgc
      361 tgccctcgat cgataatcgg cttctccaga attttgcgac tcaaggtgcg ctcgcgcttg
      421 aacgggcacg cttggttcat ggcggaaatc aataagtgtc attcccgcat gctttaagcg
      481 ggaatccagc aactctatta gcgtaatgcg tttactgtca atctggatgc ccgacacaca
      541 tgcggtaaag cacgcagtgg gtaccgaaaa cattcgggca tgacatgcta aaatcacttt
      601 atgagcgatc aacgtcccga tcccgatgaa ctcttagcgc gcgtcaagca cgaagagagc
      661 aaacgcttgc gcggccgctt gaaaattttc ttcggcgcga cagccggcgt gggtaaaacc
      721 tacgccatgc tggagcaggc gcgtgccatg aaagccaagg gcgttgatgt tctggccggt
      781 gtcgtcgaga cacacggccg cgccgagacc gaagcaatgc tgcaaggtct cgatattttg
      841 ccgccgcgtt tggtggacta tcgcggcacc caacttcagg aattcgatct cgatgccgcg
      901 ctgcaccgtc accccgcttt aattctcctc gacgaattgg cgcatacgaa tgcgactgga
      961 tcgcgtcatg ccaagcgctg gcaggatgtt gatgatctac ttacggtcgg tatcaacgtc
     1021 tacaccacaa tgaacgttca gcacatggaa agcctgaacg acgtcgtggc gcagatcacc
     1081 aacgtcgtcg tgcgcgaaac cgtgccggat tcgatcgtcg agcaggccga cgaagtggaa
     1141 ttgatcgatc tgccgccgga tgaattgatc cagcgcttgc gcgaaggcaa agtttatgtg
     1201 tcacagcaag ctgagcaggc gatcgaaaat ttcttccgca agggcaactt gattgcgctg
     1261 cgtgaattgg ctttgcggcg cacggccgat cgagtggacg cgcagatgca aatgtaccgg
     1321 cgcgatcatt cgatcgcgac gacgtggccg accaacgaac ggttattggt ctgcattggg
     1381 ccaaatcctt tagcgacgcg gttggtgcgc gcggcgcgcc gcatggctgc cggtctgcat
     1441 gctgagtggt tagtcgtcta tgttgaaacg cccggccact tgcgcttgcc tgaagccgat
     1501 cgcaaccgcg tgatcgatac cctgcgtcta gcggagcggc tcggtgcaga gacgatcacg
     1561 ctcagcggcc agactgtcag cgaagaaata atcaactacg cacgacggcg caacatcagc
     1621 aagatcgtcg tcggcaagcc gcatcgatca ccctggcgac aatggctacg aggatcgatc
     1681 gtggatgcga tcatccgctc tagcggcgag atcgatgtat atgtcatcac cggcgatcgc
     1741 ggcgaacccc gatcgatcca acgtgcgatc gggctgcggc gaactagcag ttggtcagct
     1801 tacgcttggg gcatggtgat cgtactggga tgcaccggac tcgccggact gcttcagccg
     1861 ctccttgatc cattgaattt agtcatgatc tatttattgg gtgtcgtgct gactgccgtg
     1921 cgctatggac gcgggccttc gattctagcc actatcttga gcgtagccgc gttcgacttc
     1981 ttcttcgtac caccgtttct caccttcgct gtatcagata cacagtacat catcacgttc
     2041 ggtgtcatgt tggtcacggc actgattatt agcaacttga catggcgcac acgccagcaa
     2101 gccgaagctg cgcgtcaccg tgagcggcgc accgctgcgt tatatgcgat gggacgcgaa
     2161 ttggtcagca tgcgcggcat tcaaaacctg gcgagcgcgg cactgcggca tgtcaatgaa
     2221 gtcttcaact gtcaggttat catcctgctg cccgatgcga atggagaaat gacttcaccc
     2281 gtgctgccca caaattttgc aatagatgtc aaagagcaaa gcgtggcgca gtgggtgttc
     2341 gatcgcagcc agatggcggg taaggataca gatacgctgc ccagtgccac ggctctatac
     2401 ctaccgatga tcgcgtcgcg tgggacgatc ggtgtcctgg gcgtgcgcac gatagacaag
     2461 ctcaatctgt ctgaaccgga acagctgcat accctcgaag cgtttgccag ccaggcggcc
     2521 ctggcgatcg agcgggcgcg cttatccgat gaagccgagc aagccaagat ccaagtcgaa
     2581 acggaacgcc tgcgcaattc gctgctcagt tccgtctcgc acgatctgcg aacaccgctg
     2641 gcctcgatta gcggtgcggc cagcagtttg ttggaagctg atttgctcga tgctgaaacg
     2701 cgccgcgatt tggccgaagc gatctatgag gaagccagcc ggctcgatcg attagtgcgc
     2761 aatctattgg atatgactcg cctggaatcc ggcgcgatta agctgcagaa agaatggcag
     2821 ccgttggaag aagtgatcgg cgtggtcttg gcccgattgg cggatcaatt gaaagatcga
     2881 tcggtgtcca cccacctgcc gatcgatctg ccgctcgtcc cgctcgatgg agtattgatt
     2941 gaacaagtct tgctgaactt gctggagaac gcgatcaagt acacgccggc cggaagtccg
     3001 atcgatcttt cagcgtgggc ggatcatgat caagtcacga tcgagattgc ggatcgaggg
     3061 ccgggtttgc ccgtcggtga agagcagcgc atctttgaaa aattttatcg cgcgcaaaaa
     3121 ccggaaacac atggggttgg cttgggcttg acgatctgtc gcggcatcgt cgaggcgcat
     3181 ggtggccgca tttgggccga gaaccgatcg ggcggcggaa caacctttcg ctttacactg
     3241 ccgctcgaag gcacgccgcc aaaaatcgat agcaatatca aatccatcgc tgctgagtca
     3301 tcatgatcac atctgcattg ggtgatttgg ttttagtaat agaagatgag ccacagattc
     3361 ggcgcttctt gcgcgcgacg ttggtcagcc acggctatcg ttttgccgaa gcggcgacgg
     3421 cgcagctcgg tttgatgcaa gcggctacct ataacccgca ggtcatcatc ctcgatctcg
     3481 gtttgcctga cttcgacggc ttggaagtta cgcgccgact gcgcgaatgg acgactgtgc
     3541 caatcattgt gttgtcggcg cgcggtcaag agcaggataa gattgccgct ttggatgcgg
     3601 gtgcagacga ttatttgacc aagccattcg gcgtgggcga attggtcgcg cgcattcgag
     3661 tggcactacg gcatgcggcg cgagcggcag atcccgcaca tcaatcgagc ttcacggccg
     3721 gcaacattca tgttgatctg gatcagcggc gcatcttcgt cgacaaggct gaagtccacc
     3781 tgaccccgat cgagtatagc ttgcttacca cgttgattcg ttacgcaggc aaagtggtca
     3841 cgcatcatca actcttaaaa gaagtgtggg gcgaagccta tgctgatgaa agtcattacc
     3901 tgcgcgtgta tatgggtcag ctccgccgca aactcgaagc cgattcgaca cgaccgcgct
     3961 atctgttgac cgagccgggt gtcggctatc gattggtgat ggatgaatag ttattgtctt
     4021 ctgatcctcc cttcggtttc cccttcatct ccaagttcta accgctaacc tttaacctcc
     4081 aatattttta tttgttcttt atgcagtcga gagaatcctt tgcggctgtt ttatatccac
     4141 ctgttaagat gaactgggcg caattcgaat tcactgaatt gcttgactgg tgatcaacaa
     4201 tgttgttctc gataatctta ttggttgtgg tttgctttct ggcctataaa gtttctccca
     4261 gagaaaacaa agagcatgcg catgtccacg gtgcaggcca gatcgccttg ctggcagcgc
     4321 tggctctgtt tggtgtcgat tactttacct catacttcta cgctaccggc gagatgatga
     4381 gcgcactgca tccctatggg ttacagaact atggctacat cgccgtattg gtcatcggtc
     4441 tggcgaactt cgtattcggc ggcttataca tttactcgct gggggtgttc aatgaaggcg
     4501 gcggatccta taccgcgtca atgcgttacc tggcgccgac acttagcttg atcgtcgccg
     4561 taacgttgat cgaagattac gtgctcacga tcgtcgtctc ggccttatcg ggcggcgatc
     4621 agctgttgtc tgctttaggc gcgtatggga cgacatggat ctggcatttt gtcattggcg
     4681 cgtccttggc agcggtcact tggtatctca ccattcgcgg acgcggcgag tcatcgcggg
     4741 tggtcttcac gctattgggt atcttcggct tattgacatt gattatggcg atcggcctga
     4801 tcgttgctaa cgttaaaggt gttccagcat tgccagccga tcaagcgacc gaagcggcct
     4861 ctttaggtca agccatcctg catttattga cggcctcgat gaaaggcatg gtcgcgttga
     4921 ccggcctgga agccatgtcg aacggcattc agttcgtgat caatgaagat gcaggtattg
     4981 tgaagtgggg caagcagcat ctgccgaagt tcaagaaact atggaacttt tacagcggca
     5041 aatcaggcat tgggcgcctg gttcaatcct cgttcttgtt ctacggcggc ttaaccacgt
     5101 tattcttaac cttctttgcg attcgcttca atgtatttga cggcacagcc ggccgcactc
     5161 ttgtcggcaa cctggccttc atcggtttca ctcagattcc tggcggagag attttgtttt
     5221 gggcatatca gctcctggcc gtagcattgc tggccgctgc ctcgatgacg gcctttcaag
     5281 a
//
LOCUS       DASRTJ010000052         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_3255,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000052 DASRTJ010000000
VERSION     DASRTJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFF70_01000"
     CDS             complement(<1..864)
                     /locus_tag="VFF70_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZY43300.1"
                     /translation="MIQLLIDNPLLLLFVVAAIGYPLGRIKFRGGSLGVAAVLFAGLA
                     IGSLHPDLKLPEIIYLLGLVLFVYTVGLSSGRGFFASFRRKGLRDNFLVMVVLIVGAG
                     LALIAHFVLNLKATLTAGLFAGSLTNTPALAGVLETIKSIAPAGAVDRLLAEPVVAYS
                     VSYPIGVVGMILAMAVVQRIWKIDYAAEALTLRDVGATQRRLSNRTIRITRSEAIGMP
                     IHEILRDKPWDVIFGRMKHADQLSLPHGWSVLALDDLITAVGSPEDLDRVTEFLGEAS
                     DEQLDLDRSQLD"
     gene            1028..1870
                     /locus_tag="VFF70_01005"
     CDS             1028..1870
                     /locus_tag="VFF70_01005"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HZY43301.1"
                     /translation="MSRLSNSWELVKESWKVLRADKELLIFPIVSMIGSIIVTIAFMV
                     PIFLSRLYESFSGESSGNKIAFVAFMFLFYVVEYTVIFFCNAALVGAAMIRLKGGDPT
                     LRDGFRIASQHFGAIIGYAIISSTVGMILRAIAERGIIGQIVSGFIGFAWNVATYIAV
                     PILVVEGVGPIEAVKRSTAYLKKTWGERIIGNAGIGLVFGLIIFLIILLGVPAMIALA
                     STKSIGLIAFGGILLVTIIIGLGLIASALTGIYTAAVYRYAAEGATDGFFDPNIVQNT
                     FRQK"
     gene            1972..>2841
                     /locus_tag="VFF70_01010"
     CDS             1972..>2841
                     /locus_tag="VFF70_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZY43302.1"
                     /translation="MEMRLCLMGFGNVGRELVKLIDRKRDEIKAQYDLTFTIVGVATG
                     SHGLLNDRTGLDHAFLLGGEWGDRAKRGTDQDRLHLIKDCGADALIEMSPIDRNAGRP
                     AIDYLEAALNLNMHAITANKGPVVHAYQQLSELARQKRRAFLFEATVMDGFPLLNLYR
                     ECLPATKVSGFRAVLNSTTNLILTLMEEGKSFDEAVQHAQSIGIAETDPSNDIDGWDS
                     AVKVCVLSNVLMGADIRPGDVDRSGIRGITAEMIATAQQNRRKWRLLCSADWIDGKLI
                     ARVAPEDVPANDPL"
BASE COUNT          680 a          768 c          771 g          622 t
ORIGIN      
        1 atctaattga gatcgatcta gatccagctg ctcatcactg gcctcgccca gaaattcagt
       61 cacccgatcg agatcctccg gcgatccaac agccgtaatc aaatcgtcga gcgcgagcac
      121 cgaccagcca tgcggcagtg ataattgatc ggcgtgcttc atccggccaa agatcacatc
      181 ccacggcttg tcccgcaaaa tttcatgaat cggcataccg atcgcttccg atcgggtgat
      241 gcggatcgtt cggttggaca aacgccgctg agttgcgccg acatcgcgca gggtgagcgc
      301 ctcggcggca taatcgatct tccatatacg ctgcaccacc gccatcgcca gaatcatgcc
      361 caccacgccg atcggataac tgactgaata agccacgacc ggctcggcca acaatcgatc
      421 gaccgcacca gccggcgcga tgctcttgat cgtttccagc acgccggcca gtgccggtgt
      481 gtttgtcaag ctgcctgcga acaaacccgc cgtcaacgtc gctttgagat tgagcacaaa
      541 atgcgcgatc aacgccagac ccgcgccgac gatcaagacc accatcacca aaaagttatc
      601 gcgcaagcct ttgcgtcgaa acgaagcgaa gaagccgcgg ccacttgaaa gtccaacggt
      661 gtacacaaac aataccaagc cgagcagata aatgatctcc ggcaatttga gatcaggatg
      721 cagcgatccg atcgccagcc cggcgaacag aaccgcggca acgcctaaac tgccgccgcg
      781 aaatttgatg cggcccagcg gatagccaat cgccgcgacg acgaacaata agagcagtgg
      841 attatcgata aggagttgaa tcataatgaa aggcagctct gcggattgca atgcacaaat
      901 tatcaactga tcgtaacaag attatagcat tgcattttca aattgaagca aagatctcta
      961 tctttacaca tatttcattt cgtcaagata atggtacaat gcgcgtaaca cactgaggag
     1021 gcacaccatg tcacgcttat caaacagttg ggaactcgtc aaagaaagtt ggaaagtcct
     1081 gcgcgccgat aaagaactgc tcatctttcc gatcgtatcg atgatcggat cgattatcgt
     1141 cacgatcgcc ttcatggtgc cgatcttcct ttcgcggcta tatgagagtt tctccggtga
     1201 gagcagcggc aacaagattg cgtttgtggc cttcatgttt ctgttttacg ttgtcgagta
     1261 cacggtcatc ttcttctgca acgcggcgct ggttggtgcg gcaatgatcc gcctgaaagg
     1321 cggcgatccg acgctgcgcg atggctttcg aatcgcgtcg cagcactttg gcgcgatcat
     1381 cggctatgcg atcatctcat cgacggtcgg catgatcctg cgcgcgattg ccgagcgcgg
     1441 catcatcggt cagatcgtgt cgggcttcat cggcttcgcc tggaatgtgg cgacgtatat
     1501 cgccgtgccg atcttggtcg tcgaaggcgt cggtccgatc gaagcggtta aacgcagcac
     1561 agcttacttg aagaagacgt ggggcgaacg aatcatcggc aacgccggga tcggccttgt
     1621 gttcggcctg atcattttcc tgatcattct gctgggtgtg cccgcgatga tcgcgctggc
     1681 ctccactaaa tcgatcggct tgattgcgtt cggtggaatt cttttggtca cgatcattat
     1741 cggtctgggg ttgatcgcct cggcgctgac cggcatctac accgcggcgg tttaccgcta
     1801 tgcggctgaa ggcgcaaccg atggtttctt cgatccgaat atcgtgcaga acacatttag
     1861 gcagaaatag ccacgggcgg atagcaaata ccagattgaa gattgtggcg cagataaacc
     1921 ctgcgccatt ttttctaact cgcacaaatt tctagaaggc ggagccagcg catggaaatg
     1981 agattatgct taatgggttt cggcaacgtg ggccgcgagt tagtcaagtt gatcgatcgc
     2041 aagcgcgacg agatcaaagc acagtatgat ttgactttca cgatcgtcgg cgtcgcgacc
     2101 ggatcgcacg gcttgctgaa cgatcgaacg ggactcgatc acgccttcct gctcggcggc
     2161 gaatggggcg atcgagcgaa acgtggcact gatcaagatc ggttgcactt gatcaaggac
     2221 tgcggcgcgg acgcattgat cgaaatgtcg ccgatcgatc gcaatgcagg tcgcccagcg
     2281 atcgattatc tcgaagccgc gctgaatcta aacatgcatg cgatcacggc gaacaaaggt
     2341 ccggtggtgc atgcgtatca gcaactcagc gaactcgctc gacaaaagcg tcgagcgttt
     2401 ttgtttgaag ccacggtcat ggacggtttt ccgctgctga atttgtatcg cgaatgtttg
     2461 cctgcgacaa aggtgtcggg ttttcgcgcg gtcttgaaca gtaccacaaa tttgattctc
     2521 actttaatgg aagaaggcaa gtcgttcgat gaggccgttc agcacgccca atcgatcggg
     2581 atcgccgaaa ccgatccaag caacgacatc gatggttggg attcagccgt aaaagtctgc
     2641 gtcttatcga atgttctgat gggcgcggac atccgaccgg gcgacgtgga tcgatcgggc
     2701 atccgcggga tcactgctga aatgatcgcg accgcgcaac agaatcgacg caaatggcgc
     2761 ttgctgtgct cggcagattg gatcgatggc aaattgatcg ccagagtcgc gccggaagac
     2821 gttccggcca acgatccgct t
//
LOCUS       DASRTJ010000053         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_305901,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000053 DASRTJ010000000
VERSION     DASRTJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305901"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFF70_01015"
     CDS             complement(<1..546)
                     /locus_tag="VFF70_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZY43303.1"
                     /translation="MPNVALADALSPLILTLDIGSSSTRAMIFDARARSIDDLEAREV
                     HTLHTEPDGTAIDHADELVHRVVAVIDRVIAMLGDRSSDIGAVAIDTYASNLLGLDRS
                     SGPITPIYTYADTRPTDDAARLRAKYDETTVLNRTGCLVRSSYLPARFMWLARTQPKL
                     LQEVNRWISIGEYLFDQFFDRS"
     gene            complement(618..>2252)
                     /locus_tag="VFF70_01020"
     CDS             complement(618..>2252)
                     /locus_tag="VFF70_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZY43304.1"
                     /translation="AVTLVTTFVPQGLVLAATLALSFGAVRISLQKTLVQRINAIESM
                     ANVTVLCFDKTGTLTENRLSVTEIIPIGSRTLDEIRPMLARYIGQLATHNKTASAIEA
                     FVGKAIDGPEKQSEIPFTSTRKWGAMTFADGQTFLLGAPEMLVDDNSIHATSATFAKL
                     GLRVLAFAVSRVKPIGSELPADRSPVALIVVHDTPRADIRSTLASFTARNVRLKVISG
                     DNAETVSSIAAAAGMNVSRVVTGPELEGLTGTAFEEMVLSADVFARIKPETKRQIISA
                     LGDTGEYVAMVGDGVNDVPALKAARLGIAMFDGAQIAKDVADLVLLNNALSTLPQALT
                     EGHKTTQKIYATAKIFLTRNLYFILMFIMVGFMGLPFPGQVRQLSWASITTSGIPAML
                     IALEWIQPRTIWKFRRQVLGYILISGLIGAVAVTTAYTVTYLASGHDLMLARSVMTLM
                     AVIYGTLIFWDVNGVIPFEPITFKQNMREAIIGVLVAMVTLTVPFLLPRLFEIAPIPA
                     PYLIGVSMLAILAAFALWRSTLAQSNILAPFKSLVE"
BASE COUNT          518 a          611 c          647 g          476 t
ORIGIN      
        1 cgatcgatca aagaactgat cgaataaata ttccccgatc gaaatccagc ggtttacttc
       61 ttgaaggagt tttggttgcg tacgcgccaa ccacatgaag cgcgccggga gataactcga
      121 tcgcaccaag cagccggttc gattcagcac agtggtttca tcatattttg cgcgcagccg
      181 cgccgcatcg tcggttggac gagtgtccgc ataagtgtag atgggcgtaa tcgggccgga
      241 agatcgatcg aggccgagaa gatttgaagc gtacgtatcg atcgccactg cgccaatgtc
      301 cgacgatcga tcgcccagca tggcgatcac tcgatcgatc accgcgacaa ctcggtgtac
      361 tagttcgtcg gcatgatcga tcgcagtgcc gtcaggttcg gtatgtagag tgtgaacttc
      421 gcgtgcctca agatcatcga tcgatcgcgc gcgggcatca aagatcatcg ctcgcgtaga
      481 cgatgatccg atatcgagtg tgaggattag tggggagagc gcatctgcta gcgcaacgtt
      541 tggcatagct gcgagtatag ggccaacgga acttgttata agcaggccga gtttagcgaa
      601 aagcgatacg caattgctta ttcaaccaac gatttaaatg gcgccagaat attcgattgt
      661 gccaaggtgg atcgccacag cgcgaaggcg gcgaggatcg ccagcatgct gacgccgatt
      721 aaataaggcg cggggattgg cgcaatctca aatagtcgcg gcagcaagaa cggcaccgtc
      781 agcgtcacca tcgctaccag cacaccgatg atcgcctcgc gcatattttg cttgaaagta
      841 atcggctcga acggaatcac cccgtttacg tcccagaaga tcagcgtgcc atagatcaca
      901 gccatcagcg tcatcaccga tcgggccagc attaagtcat ggccggaagc taaataagtg
      961 acggtgtaag cggtggtgac agcgactgca ccgatcaagc cgctaatcaa aatgtagccc
     1021 aaaacttgtc ggcggaattt ccagatcgtg cgcggctgaa tccattcgag cgcgatcaac
     1081 atcgctggaa taccgctagt ggtgatcgat gcccaactga gctgccggac ttggccgggg
     1141 aagggcaagc ccatgaagcc gaccatgatg aacatcaaaa taaaatacag gttgcgcgtc
     1201 aaaaatattt tggcggtcgc ataaattttt tgtgtggtct tgtgaccctc ggtcaaggct
     1261 tgcggcaaag ttgagagcgc gttgttcagc agcacgagat cagccacatc tttggcgatc
     1321 tgcgcgccgt cgaacatggc aatgccgagc cgcgcggctt tcaatgcggg cacatcattc
     1381 acgccgtcgc cgaccatcgc cacatattcg ccggtgtcgc ctagcgctga aatgatctgg
     1441 cgtttcgttt cgggtttgat gcgcgcaaac acatcggcgg atagcaccat ctcttcaaag
     1501 gcagtgccgg tcagcccttc caattccggt ccggtcacga cgcgcgagac gttcatgccg
     1561 gccgctgctg cgatcgagga gacggtctcg gcattgtcac cgctgatcac tttcaagcgc
     1621 acgttgcgtg ccgtgaacga tgccagggtc gatcgaatgt cggcgcgcgg ggtatcatgc
     1681 acgacgatca acgcgaccgg cgatcgatcg gcggggagct cagatccgat cggcttaact
     1741 cgcgaaaccg caaaggccag cacgcgcaag cctagcttcg caaatgttgc gctggtggcg
     1801 tggatcgaat tgtcgtcgac caacatttcc ggcgcgccga gcaagaaggt ctgcccatcg
     1861 gcgaaagtca tcgcacccca tttgcgcgtg gaggtgaagg gaatttccga ttgcttctct
     1921 ggtccgtcga tcgctttgcc gacaaaggct tcaattgcgc tggcggtttt attatgcgtg
     1981 gcgagttggc cgatatagcg cgcgagcata ggccggattt cgtcgagtgt ccgtgatccg
     2041 atcgggatga tttccgtgac ggacaatcga ttctcggtca gcgtgccggt cttatcgaag
     2101 cacaacaccg tcacattcgc catcgactcg atcgcgttga tgcgctgcac cagcgttttt
     2161 tgcagactga tgcgcactgc gccgaatgac aacgccagtg tcgcggccag caccagacct
     2221 tgcggcacaa atgtggtgac cagcgtgact gc
//
LOCUS       DASRTJ010000054         7089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_3356,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000054 DASRTJ010000000
VERSION     DASRTJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3356"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5457)
                     /locus_tag="VFF70_01025"
     CDS             complement(<1..5457)
                     /locus_tag="VFF70_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZY43305.1"
                     /translation="MTTPLKVLLVEDNAADAELILHALRRAGFDPDVRRIDQASELQA
                     ALDPSLDLILTDYQLPQFTGLHVVKTVRAAGSDVPVIIVSGAIGEEVAVEALRQGADD
                     YLLKDRLGRLDSAVTNALAQRRLRQEKRVSDEALRESEAHLKNILDNASEAIISVDEA
                     QRITLFNSRAEKMFGYRVEEIIGQPLDLLIPAALVSIHRQHAHDFVHLDNTGHLMRMG
                     RELTCRRKDGSEFPGEIGLSRLIENGQVTITAIVADITERKQAEAAVRESKSSLQAVL
                     QSTADGILAVGSDNEVLYSNERFAEMWRIPPTVIADKDDVVLLQHVLDQLVDPQSFLQ
                     KVQELYRSVEESFDSLYFKDGRVFERLSQPLIQESKLHGRVWSFRDITSRKQAEAERQ
                     TLLEIMQAAATTEDLHSFLGLVHQSVAKVIWAENFFVTLYNQTTGLFEEAFSIDQHDQ
                     SPTPPARFEKSISAYVFRTGRPLLMNQAQFDVLQDAGEVELIGANSPSWLGVPLKTAR
                     GTIGVMVVQDYEQADRYSERDQNFLTSIAAQAALAVERKRAEEALLESEKRFRALIES
                     SSDAITLLGATGKVIYDSPSAPGMLGYGPEDWIGRDVFALIHPEDLPRIQGLFEHLRQ
                     TAGARVNFTLRTRHKSGSWLWLEMVATNLLAEPGVNAIVLNYRDVTERKRAETLQNAV
                     YDIAQASALADSLTNLFPQIQRIISGIMPAENFYIALYDQKHNQLSFPYSIDEVDQTY
                     TGEEIEPGRGLTAYVLRTGKSLLCTGSIHKQLVEQGELEQIGVSMQIWLGVPLIVQAK
                     VIGVMVVQHYSDPQAYGEREQRVLEYVSGQVALAIDRKQAEEALAASEAELRALFAAM
                     HDAVLVIDRAGVYREIAPTNLESDLELHRLSPQELLGKKLQDVFPAEQAETFYNVIRR
                     VLATQQIEQIEYELNFNSQAVWYEASISPMSADSTLWVARDITERKRAEEVIQKTESD
                     YRYLFNNANDAIIIFEPEQEIILEANAAACAIYGFAHAELVGLSLKKLTKDLTKGETQ
                     VHELLEMGSYHDFESIHLNRKGEQINFLINSSVIEYQGRPAILSLNRDITERKRAEEL
                     LRTKVEILQALSEIEHEIIATNDPQGILDLVCRRAAALVHVSKSVITSIGPSGDLELA
                     AHFGLSDPAGFNTIFKRLRQTRRLGGISLAARQMVVLPDPSAMDGPLSELAAGENINA
                     AVIVPLAAYEKATGALVVFNAVPRAWTTDEVSVLNTLAGQAAIALQNARLFQEINRRA
                     ERLALLNRISHAVSMTLDIDELLEIVYREVTAKVESEVYLAALYDDQTNELDFRIRVD
                     RGVRQPPERHPLRTGMSGLIIGTKQPVLVRDFEREKAQLPPVSLWGSMEAPRSWLGVP
                     MLLGERVLGVIAVQHYRPNIYNEDDQRLLVTIADSVAVAVENARLYTMQERRAARLAE
                     IVKLGIELGTLQDEYLMLKTLVARTAAIAQSDTCTVLWKEQTNVLTLAAQIGLPENTP
                     LGLPISLPDLPFIHRTLSNGEPLILSDIDQQAPAVRALLVRSDLHSFFAYPMLGLKEI
                     IGVITLSYIVAHKPSQEEINAYQLLAHRAAGAIEDARLFHTEHEQRTWAEALSDTAAA
                     LNATLDFDELLDQILANVGRVVPHDAASIMVSESGVARVVRYRGLAERDVVEVTAQLR
                     LQVDEVDDLRRMSETGQAVIIEDTHAASGWTAVPETNWLASSLGAPIRVKGQVIGFIS
                     LGSAVPKFFTPMHAARLQSFADQAASALHNAQLLAETQQRAEQFTLLHETVRDLQFQP
                     DVPTLLGIIVDRAKLLLHAPGGFIYLYDAAHSELE"
     gene            complement(5608..6051)
                     /locus_tag="VFF70_01030"
     CDS             complement(5608..6051)
                     /locus_tag="VFF70_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43306.1"
                     /translation="MTVGKVEILLIEDNPNDVELTLRAFKKNNFTNTVHVVRDGAEAL
                     EFLFATGAYADRDTNDTPKVILLDLKLPMVDGLEVLRRIKSDAHLRVIPTVMLTSSRE
                     ERDVVESYHLGVNSYIVKPVDFQQFTEAIRTFGLYWLLLNHPPEK"
     gene            complement(6067..>7089)
                     /locus_tag="VFF70_01035"
     CDS             complement(6067..>7089)
                     /locus_tag="VFF70_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43307.1"
                     /translation="GFDWRYLYLNDVADKHNRRPKAELLGKKYMEMWPGVEATEVFTV
                     IRRCMEEHEPQSMETKFIFPDGGTGWFELRIYPVPEGSVIFSIDVTERKGAEEEIQTL
                     NAELEQRVIERTAQLEAANKELESFSYSISHDLRAPLRAIDGFSRILVEDFSANLPPD
                     LQRYLQLIRGNAGQMGHLIDDLLQFSRLNRQPLNKQMVKPMQVVREALRALQPEQAVH
                     QPRIIIGELLECEADPALLKQVWINLLSNAIKYTRKQAAPQIEVGLKYVDGATVYFVR
                     DNGAGFDMQYAHKLFGVFQRLHRAEDYEGNGVGLAIVQRIIERHGGRIWAEAMPNVGA
                     TFYFTL"
BASE COUNT         1554 a         2037 c         1877 g         1621 t
ORIGIN      
        1 ctctaattca ctgtgcgcag cgtcatataa atagataaag ccgcctggcg catgcagcag
       61 taatttagct cgatcgacaa tgatgcccag caaagtcggc acatccggct gaaactgtag
      121 gtcgcgaacg gtttcgtgca gcagagtaaa ttgctcggcc cgctgctgcg tctccgccaa
      181 cagctgagcg ttgtgcaacg cgctggccgc ttgatcggca aatgattgca aacgagcggc
      241 gtgcatcggc gtgaaaaact tcggtacggc gctacccaac gagatgaaac caatcacttg
      301 ccctttgact cgaatcggcg cgcccaagct ggaggcaagc caattcgtct ccggcacagc
      361 cgtccagccg gatgcagcgt gagtatcttc gatgatcacg gcttggcctg tctcgctcat
      421 ccggcgcaga tcgtccactt catccacttg gaggcgcagc tgcgcagtaa cctcgaccac
      481 atcgcgttcc gccaagccgc gatagcgcac tacccgcgca acgcctgact cgctgaccat
      541 gatgctggcc gcgtcatgtg gcaccacccg gcctacgttg gccaggattt gatctaagag
      601 ttcgtcaaaa tcgagagttg cgttcagcgc agccgcagta tcgctcagtg cttcggccca
      661 ggtgcgttgt tcgtgctcgg tgtgaaacaa gcgggcatct tcgatggcac cagccgctcg
      721 atgggccaga agttgatatg cgttaatttc ttcttgtgat ggtttgtggg cgacgatata
      781 actcagggtg ataacaccga taatctcctt caagcccagc ataggatagg caaagaagga
      841 gtgtaggtct gaacggacga gcagcgcacg aaccgccggc gcttgttgat cgatatccga
      901 caggataagt ggctcaccgt tgctcagtgt ccgatggata aacggcaggt ctggcagcga
      961 gatcggtaat cccagcggtg tattttcagg cagaccgatt tgcgcagcaa gcgtcagcac
     1021 attcgtctgt tccttccaca acacggtgca ggtgtcactc tgtgcaatgg ccgcggtgcg
     1081 cgcgaccaaa gtcttcagca tgagatattc atcttgcaat gttcccaatt caatgcccaa
     1141 cttgacgatc tcggccaggc gcgcggcacg gcgttcttgc atcgtataca accgggcgtt
     1201 ctccaccgcg acggcaaccg aatcggcaat ggtgacgagc aaccgctgat cgtcttcgtt
     1261 gtaaatattt ggccggtagt gctgcaccgc aatcacgccg agaactcgct cgcccaacag
     1321 catgggcacg cccaaccacg accgcggagc ttccatgctg ccccacaggc taaccggtgg
     1381 cagctgagct ttttcccgct caaaatcgcg cacgagaacc ggctgcttgg tgccaatgat
     1441 gagccccgac atgccggtgc gcagcggatg gcgttcgggc ggctggcgca cgccgcgatc
     1501 gacgcgaatc cgaaagtcca gctcgttcgt ctgatcgtcg tataaagccg ccaaatacac
     1561 ttccgactcg accttcgccg tcacttcacg ataaacaatc tccaataatt cgtcgatgtc
     1621 caaggtcatg ctgaccgcgt gtgaaatgcg attgagcagc gccagacgtt cagcccggcg
     1681 attgatttcc tgaaacaacc gggcgttttg cagcgctatc gcggcttgac cggccagcgt
     1741 gttcaacaca ctcacctcat cggttgtcca agcgcgcggc acggcgttga aaacaactaa
     1801 cgcgcccgtc gctttttcat aagccgctaa gggcacgatc acggcggcgt tgatattctc
     1861 gccggccgcg agctcagata gcggtccatc catcgcggac ggatcaggca gcaccaccat
     1921 ctgacgcgcg gccaacgaaa taccacccaa ccggcgcgtc tggcgaagtc gcttgaaaat
     1981 ggtattgaag ccagccggat cactcagacc aaaatgagca gccagctcaa gatcgcctga
     2041 agggccgatc gaggtgataa ccgatttgga gacgtgcacc aaggccgcag cccggcggca
     2101 aaccaaatcc aaaatgcctt gcggatcatt agtggcaatg atctcatgtt cgatctcgga
     2161 cagtgcttgc aagatttcga ctttggtgcg gagcaattct tccgcccgct tgcgttcggt
     2221 gatgtcacgg ttcaggctga ggatggccgg tctcccttga tattcaatca ccgaggagtt
     2281 gattaggaaa ttgatttgct cccctttcct attgaggtgg atgctttcga aatcatgata
     2341 ggaccccatt tccaacaact catggacctg cgtctcgcct ttggttagat ccttggtcaa
     2401 ttttttcagg ctcagaccga ccagttcagc gtgagcgaag ccgtaaatgg cgcaggccgc
     2461 cgcgtttgct tcgaggatga tctcttgctc aggctcgaag ataataatcg catcattggc
     2521 attattgaag agataccggt aatcgctctc ggttttttgg attacctctt ccgcccgttt
     2581 gcgttcggtg atgtcgcggg ccacccagag tgtgctgtct gcactcatcg gcgagatgga
     2641 tgcttcgtac cacaccgctt ggctattgaa gttgagttcg tattcgatct gttcaatctg
     2701 ttgcgtcgcc aggacacggc ggatcacatt gtaaaaggtc tcagcctgtt cggccgggaa
     2761 gacatcctgt aatttcttgc ccagcagttc ttgcggcgac agacggtgca attcaagatc
     2821 ggattcaaga ttggtgggcg cgatctcacg ataaacaccg gctcgatcaa tgaccaatac
     2881 agcgtcatgc atggcggcga acagcgcgcg gagttcagcc tcggaggccg ccagcgcttc
     2941 ttccgcttgc ttgcggtcaa tcgccaaagc aacttgcccg gacacgtatt ccagcacgcg
     3001 ctgttcccgc tcgccatatg cctgtggatc ggagtagtgt tgaacgacca tgacaccgat
     3061 gaccttggct tgaactatca gtggaacacc cagccagatc tgcattgaaa cgccaatctg
     3121 ctcgagctcg ccctgttcca ctagctgctt gtgaattgag cctgtgcaca ggagagactt
     3181 gccggttcgt aggacatagg cggtcaatcc tctgccgggt tcgatctcct cacccgtata
     3241 ggtctgatcc acttcatcaa ttgaatatgg gaagctcagc tgattgtgtt tttggtcgta
     3301 gagagcgata taaaagtttt ccgccggcat aatcccggaa atgatacgtt ggatctgtgg
     3361 gaacaggttg gtgagtgaat cggcaagcgc ggatgcctga gcaatgtcgt atacagcatt
     3421 ctggagagtt tccgcccgct tgcgctcggt gacgtcgcgg taattcagaa cgattgcgtt
     3481 tacgcctggc tcggcgagca aattagtcgc gaccatttct agccacaacc acgatccgct
     3541 tttatggcga gtgcggagcg tgaagttgac acgcgcgccg gccgtttgac gaaggtgttc
     3601 gaaaagacct tggatcctgg gcaaatcctc gggatggatc agcgcgaaga catctcgccc
     3661 gatccagtct tcaggcccat aacccagcat tcctggagca gacgggctat catagataac
     3721 tttgccagtg gcccccagca gggtgatcgc atcgctgctg ctttcgatca atgcgcgaaa
     3781 acgtttctca ctttcgagca atgcctcttc cgcccgcttg cgttctacgg ccaacgcagc
     3841 ctgcgcggca atggaggtca agaaattttg atcgcgctcg gaatagcgat cggcctgttc
     3901 gtagtcctgc acaaccatca caccaatcgt tccccgcgcg gtcttcaacg gcacgcccag
     3961 ccacgaaggc gagttggccc cgatcaattc aacttcaccg gcgtcctgca atacatcgaa
     4021 ttgtgcttga ttcattaaga gtggccggcc agtgcgaaac acataggcgc tgatgctttt
     4081 ttcaaaccgg gccggcggag ttggactttg atcgtgctga tcgatcgaaa aggcttcctc
     4141 aaacaatccg gtcgtctggt tgtataaagt cacaaagaaa ttctcagccc aaatcacctt
     4201 ggccacggac tgatgcacga gtcccaaaaa actgtgcaag tcttcggttg tggctgcggc
     4261 ctgcatgatt tccaacagcg tctgacgctc ggcttcagcc tgcttgcgac tggtaatgtc
     4321 gcgaaatgac cacacccggc catgcagttt tgattcctga ataagcggtt gggaaagccg
     4381 ctcaaacacc cggccgtctt taaaatatag ggaatcgaaa ctttcttcaa ccgaccggta
     4441 aagttcctgc accttctgga gaaaactttg cgggtcaacg agttgatcaa gcacatgctg
     4501 cagcaggacg acgtcatcct tgtcggcgat aacggtgggt ggaatccgcc acatctctgc
     4561 gaaacgttca ttgctataca gcacctcgtt atcgctcccc accgccagaa taccatctgc
     4621 ggtggactgt agcaccgctt gtaaggatga tttgctttcg cgcaccgccg cctccgcctg
     4681 cttgcgttcg gtgatgtcgg cgacaatggc cgtaatggtg acctgcccat tttcaataag
     4741 ccgggaaagc ccaatctcgc cggggaactc cgagccgtcc ttgcgccggc aggtcagctc
     4801 gcggcccatt ctcatgagat gtcccgtgtt atctaaatgg acaaaatcgt gggcgtgctg
     4861 acgatgaatc gagaccagcg cggcggggat gagcaagtcc aagggctgcc cgatgatttc
     4921 ttcaacgcga tagccgaaca tcttctcggc gcggctatta aacagcgtaa tccgttgggc
     4981 ttcgtcgacg gagatgatcg cttcggaagc attgtccaaa atatttttga gatgcgcctc
     5041 gctctcgcgc aaggcttcat ccgacacgcg tttttcctgc cgcaaccggc gttgtgctag
     5101 cgcgttcgtg accgctgaat ccaggcggcc caagcgatcc ttgagcagat aatcgtcggc
     5161 gccctgccgc agcgcttcga ccgccacctc ttcgccaatg gcgccggaga cgatgattac
     5221 cggcacatcc gatccggcgg cccgcacggt ttttacaaca tgcaagccgg tgaattgcgg
     5281 caattgataa tcggtcagga tcagatctaa cgacggatca agcgccgcct gcaactcgct
     5341 ggcctgatcg atccgccgca catccggatc gaacccggcg cggcgaaggg cgtgcaggat
     5401 cagttccgca tcggctgcgt tatcttcgac cagtaaaact ttaagcggtg tggtcatcta
     5461 gttttcttaa gctccgcgca aggtacagca atcttagcgg ttcgggttcc cctgcccgag
     5521 cccaacggtg tgtcccacgg gaggcttcgc acggagccgc tgccgctagt tctggctact
     5581 ttactgcaca atgtcagtat gccaccttca tttttccggc ggatgattca gcagcagcca
     5641 gtacaagcca aaagtgcgga tcgcttccgt gaattgttga aagtcgaccg gcttgacaat
     5701 gtaactattc acgcccagat gatagctttc gacgacgtcc cgctcttcgc gcgatgaggt
     5761 gagcatgacc gtcgggatca cgcgcaagtg cgcatcagat ttgatccgcc gcagcacttc
     5821 caggccatcg accatgggca gcttcaagtc gagcaagatt acttttggcg tatcgttggt
     5881 atcccgatcg gcatacgcgc cggtcgcgaa cagaaactct aacgcttcgg ctccgtcacg
     5941 cacgacgtgc accgtattcg tgaagttatt ttttttgaaa gcccgcagcg ttaactcaac
     6001 atcgttcgga ttatcttcga tcagcagaat ctcaacttta ccaacggtca tttccagcct
     6061 cccggattac agcgtgaaat agaatgttgc accgacgttg ggcatggctt cggcccagat
     6121 tcgcccgcca tgccgctcga tgatgcgctg cacgatcgcc agccccacac cattgccttc
     6181 gtaatcttcc gcccgatgta agcgctgaaa cacaccaaac aatttatgtg cgtactgcat
     6241 atcaaagccc gcgccgttgt cgcgcacaaa atacacggtg gctccgtcca catattttaa
     6301 gcccacttcg atctggggag cagcctgttt gcgcgtatac ttaatggcat ttgatagtaa
     6361 gttgatccac acctgcttga gcagagccgg atcggcctcg cactctagca attcgccgat
     6421 gatgatccgc ggttgatgta ccgcctgctc gggctgcaaa gcgcgcagcg cttcgcgcac
     6481 cacctgcatc ggcttcacca tctgtttgtt caacggctgc cgattcaggc gtgagaactg
     6541 cagcagatcg tcaatgagat gacccatctg gccggcatta ccgcggatga gctgcaggta
     6601 tcgctgcaag tctggtggca ggttggccga aaaatcttcc accagaatcc gcgagaagcc
     6661 gtcaatagcg cgcagcggcg cacgcaggtc gtgcgagatg gaatacgaaa acgattctag
     6721 ttctttattc gctgcttcga gctgtgccgt gcgttcgatc acgcgctgtt cgagttcggc
     6781 attcagggtt tggatttcct cctctgcccc cttacgttcg gtgacatcaa tggaaaaaat
     6841 aacactccct tccggcacgg gataaattct caattcgaac cagcctgtgc cgccatccgg
     6901 aaatatgaat ttggtctcca tggattgcgg ttcatgttct tccatgcacc gccggatcac
     6961 agtaaaaact tctgtggcct caacaccggg ccacatctcc atatattttt tcccgagtag
     7021 ttctgcttta ggcctacggt tgtgtttgtc cgcgacatcg ttgagataga ggtaccgcca
     7081 atcaaatcc
//
LOCUS       DASRTJ010000055         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_230417,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000055 DASRTJ010000000
VERSION     DASRTJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230417"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFF70_01040"
     CDS             complement(<1..241)
                     /locus_tag="VFF70_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43308.1"
                     /translation="MTLTHQLNTLESSGLIQLAQASPNLEYLFRHTLVQEAAYASLVR
                     QDRWQLHRWVGETIEQLYPDRLLSLDVTPLLARHFD"
     gene            complement(317..1327)
                     /locus_tag="VFF70_01045"
     CDS             complement(317..1327)
                     /locus_tag="VFF70_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43309.1"
                     /translation="MDTPTQIFTPPYPPSWIDRLLDWIERLPIPSWLFNLLLAPIGIL
                     GVNVLYWITGAEPVGSWAITAYGFVFIPLNLLAGGYLRQLAAHSMDQFSPALANSPKE
                     FARLKYSFTIIPQRRALIITAIVALPLNVIWLYFLKFPIVYGLFSALGYSLGGCLFFQ
                     VARQLRMIGRLHALAKHVSLFDLDPLYAFSRLTARIGFLILLVVYLSTALALIIRPDN
                     PELVYSNLAAIPFAVMCFILPLNGMHQRLAKEKTDLLAKINRQLETTLTELNRVIETG
                     ELERVDLLNRTTTSLINERDIVHKISTWPWQAATFRGFATTLLLPIFIWLITRLLTRF
                     GI"
     gene            complement(1367..>2333)
                     /locus_tag="VFF70_01050"
     CDS             complement(1367..>2333)
                     /locus_tag="VFF70_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43310.1"
                     /translation="DWIDRLRMPNWLFYLLLTIVEFLLYYGLNRVIGFPANTPLESMY
                     AYVVLYNIGGLAFIHYMRRTAKRVLEDFAPALGASDAEFMRLNYQLTVLPAIPTLIAT
                     VLGAAFTFADALDPASQALQIQYPPAVLVFAVLGLAVTGVMLLQIVRLLIMVNRIHRL
                     AQRINLFQFDPLYSFSGLTVRIGLFVVIGAYFNLAIDPNAFASPSTIALNGVLLLIGA
                     AAFVLPLNSMHQRIVQEKKRLLAEHDQRFESVVNELNQHIDARNLEQVDVLTRTMNSL
                     VIQREVLNKIPTWPWRTVTFRGFATTLLLPLVVWLITRLLSRLGL"
BASE COUNT          621 a          589 c          660 g          463 t
ORIGIN      
        1 gatcgaaatg tcgcgcgagc agcggcgtga catcgagcga gagcaaccga tcgggataca
       61 gctgctcgat cgtttcgccg acccagcgat gcagctgcca acgatcctgc cgcacgagcg
      121 aagcataagc tgcttcctgc accagtgtat gccggaagag atattccaaa ttgggcgagg
      181 cttgagccag ctggatgagt cccgacgatt caagtgtgtt aagttggtga gtgagcgtca
      241 caagagtcca gcatttgttg agatgatagt gttactcatc gaatcccgtc tgcgcggacg
      301 cgatcttcgg gcaagattag attccaaaac gagtcagcag ccgggtgatc agccaaatga
      361 agatcggcaa taacaaagtt gtggcaaagc ctctaaaggt cgcggcttgc cagggccagg
      421 ttgaaatctt gtggacaata tcgcgctcat tgatcaagct ggtcgtggtc cgattcagca
      481 gatcgacgcg ctcgagttcg ccggtctcaa tcacgcgatt gagctcggtc aaggtcgttt
      541 ccagctgccg attgattttg gctaacaggt cagtcttttc tttggccagc cgttgatgca
      601 tgccgtttaa tggcaagata aaacacatta ccgcaaaggg gatcgccgcg agatttgagt
      661 aaactagctc aggattgtca gggcggataa tcagggcaag ggccgtgctg agatagacca
      721 cgaggaggat gagaaacccg atccgcgccg tcagcctcga aaatgcatac aatggatcca
      781 aatcaaataa actgacgtgc ttggccagcg catgcaagcg ccctatcatg cgaagttggc
      841 gggcgacctg gaagaaaagg cagcccccca atgaataacc cagggcgctg aacagaccat
      901 agacgattgg aaatttcaaa aaatacagcc agatcacatt caggggcagc gctacaatcg
      961 cggtgatgat caacgccctc cgctgaggga tgatcgtgaa cgaatatttg agccgagcga
     1021 attccttcgg actgttcgcc agcgccggcg aaaattgatc cattgaatgg gcggcgagct
     1081 gccgcagata accacccgcc agcaagttga gtggaatgaa gacaaagccg taggcggtaa
     1141 tggcccagct gcctactggc tcagcccctg taatccaata caggacgttc acgccaagaa
     1201 tcccgatcgg ggcgagcagc agattaaaca gccagctggg tataggcaat cgttcgatcc
     1261 agtccagaag ccgatcgatc cagctgggcg gatagggcgg ggtaaaaatt tgcgttggag
     1321 tatccatatt caccttattc aaaataataa gtatcgagtc gtttcgttac agacctaatc
     1381 ggctcaagag ccgtgtgatc aaccagacga cgagtggcag cagcaatgtt gtcgcgaagc
     1441 ctctaaacgt tacggtgcgc cagggccatg tcggaatttt gttcaacact tcgcgctgaa
     1501 ttactaaact attcatggtg cgcgtcaaca catccacctg ctccagattg cgcgcatcga
     1561 tatgttgatt cagttcgttc acaaccgatt caaaacgttg atcgtgttcg gccaacagcc
     1621 gtttcttttc ttgcacgatg cgctgatgca tgctgttcag cggcaacaca aaggcggccg
     1681 cgccgatcag aagcaggacc ccgttgagcg cgatcgtgga cgggctggca aaggcgttcg
     1741 gatcaatggc gagattaaag tatgcaccga ttacgacgaa taaaccgatc cgcacggtca
     1801 agccggaaaa cgaatagagc gggtcaaatt ggaacagatt gatgcgctgc gccagccggt
     1861 gaatgcgatt gaccatgatc aacagacgaa cgatctgcaa cagcataacg cccgtgacgg
     1921 ccaggccgag cacggcaaag acaaggacag ccggcggata ttggatttgc agcgcttggg
     1981 aagcagggtc aagcgcgtcg gcaaaggtaa aggctgcccc cagcaccgtg gcaatgagcg
     2041 tcgggatggc gggcaggacg gtgagctggt agtttagacg catgaattct gcgtcggatg
     2101 cacccaacgc cggggcaaaa tcttccaaca cgcgtttggc ggttcggcgc atgtaatgaa
     2161 tgaaagccag cccaccgatg ttgtacagca cgacgtaggc atacattgac tccagcggcg
     2221 tattcgccgg aaatccgata actcggttaa ggccgtagta aagcaggaac tcgacgatcg
     2281 tcaatagaag ataaaacaac caattgggca tgcgcagccg atcgatccaa tcg
//
LOCUS       DASRTJ010000056         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_230437,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000056 DASRTJ010000000
VERSION     DASRTJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230437"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFF70_01055"
     CDS             complement(<1..355)
                     /locus_tag="VFF70_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43311.1"
                     /translation="MNNNFLQQAIDAIKSGQAATGKRLLIGVLQNDPFNEMAWLWMSS
                     VYTALDQRRMCLRRVLEINPANETAKRGLANLDPSTSLPTVRSYAASNAAHAEIVVPI
                     TPVNTPNWNEQDKLLV"
     gene            complement(406..1668)
                     /locus_tag="VFF70_01060"
     CDS             complement(406..1668)
                     /locus_tag="VFF70_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43312.1"
                     /translation="MSSKIGVHSLMGPRNGYGDFLQRVADAGQSLAVVKCVDDFGAAF
                     EAKKIVPNVFTVGRINDTPGADMQAFEPMTPNGYMDARKVAAWYYGLCKPIWNANKRW
                     IDAWETFNEFSAHWSWQGEFYMALMDLVERDGFKLVLYACSSGNPPDACTVFQMLPAL
                     RAAKQRGHYLSLHEYGNVGNDIPTLRGTEPYHALRYRQLYESILIPNQADPPLIISEC
                     GQNGGSTFPGTQVFMDDIKWYDANLRQDPYVIGATMFTLGNWAGANFQAALPALGDYI
                     ASTLPAPKPRQPLTPPYVFGDPPVSLPNLLHLRGTPRTQYQRIYVLLPNEPTTNDGNS
                     RTTAWIRAFLDSGTATRFRLTFGFSVDDAGIGDLDRRHVFAVNPSSWNGSLSDFFAKY
                     YHGVDYYSIEVSTPDELRNTLAKRLTPP"
     gene            2299..3468
                     /locus_tag="VFF70_01065"
     CDS             2299..3468
                     /locus_tag="VFF70_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZY43313.1"
                     /translation="MKDRKLLMIPGPIEFDPAVLAAMSLPTTSHVAANFIDVFGQALE
                     KMRKVWLSDDGQPIVIAGSGTLAMDTAGANLIEPFDKALVVNTGYFSDRFGSILERYG
                     ATVTQLRGPIAGRPTIEEVEAALKNGRFKVMTVTHVDTSTAVINDVKALAALARKYET
                     LIVVDGVCSIAGEEFRMSEWGIDVALTASQKAIGVPPGLALLVAGPRAIEALRKRESP
                     VMNYYADWNNWLPVLEAYEARKVGYFGTPAINLVWALNVSLDQILKEGLEARVERHRK
                     LSRSMQAAIKALGLGQVPLNEDCAAHTLSAPRYPQGVTSADLLPRVEAAGAIIAGGLH
                     PQIRTEYFRIGHMGSTSIGDLLATIGAVEIGLAGCGYKFEMGAGVAAAIKSNNHG"
BASE COUNT          808 a          869 c         1019 g          789 t
ORIGIN      
        1 tgacgagcag tttgtcttgc tcgttccaat tcggcgtgtt aaccggcgtg atcggcacga
       61 cgatctctgc gtgggcagca tttgaagctg cgtaactgcg caccgttggc aaggaagtgg
      121 atggatcgag gttggccaac ccgcgtttgg ccgtttcatt cgccggatta atctccaaca
      181 cacgccgcag gcacatgcgg cgttgatcga gcgcggtgta aacgctggac atccacagcc
      241 aggccatctc gttaaacgga tcgttttgca ggacgccgat caacaagcgt ttgccggtcg
      301 cggcctggcc ggacttaatg gcgtcgatag cttgttgcag aaaattgtta ttcattcgat
      361 tcagttctca cgcgttagcg ttaacccgat cggttgatcg atcgattaag gcggtgtcag
      421 ccgcttggct aatgtattcc gcagctcgtc gggcgtgctc acctcgatcg agtaataatc
      481 aacaccgtga tagtacttgg caaaaaaatc cgacagtgat ccgttccagg aactgggatt
      541 cacggcgaat acatgccggc gatccaaatc gccgatgccg gcgtcatcga ctgaaaagcc
      601 gaaggtcagc cggaagcgcg tggcggtgcc actatccaga aaggcacgaa tccaggccgt
      661 cgttcgcgaa ttgccatcat tggtcgttgg ctcgttgggc agcagcacat agatacgctg
      721 gtattgcgtg cgtggtgtgc cacgcagatg caagagattt ggcagggaca ctggcggatc
      781 gccaaataca tacggcggtg taagcggctg ccgcggttta ggggcaggca gcgtggaggc
      841 aatataatct cccaaggccg gcagcgccgc ttgaaaattt gcgccagccc aattgcccaa
      901 ggtgaacatc gtcgcgccga tcacatatgg atcttgtcgg agattggcgt cgtaccattt
      961 gatatcgtcc atgaagactt gcgtaccggg aaacgtcgag ccgccatttt gtccgcattc
     1021 gctaataatc aacggcggat cagcctggtt ggggatcaag atcgattcat acaattgacg
     1081 atagcgcagg gcgtgatacg gttcggtgcc gcgcagagtg ggaatgtcat tgccgacatt
     1141 gccatattcg tgcagagata aataatgacc ccgctgtttt gccgcgcgca aagccggcag
     1201 catttggaaa acagtacagg catccggcgg attgccgctg gagcaggcgt agagcaccag
     1261 cttaaagcca tcgcgttcaa cgagatccat cagtgccata tagaattcgc cctgccagct
     1321 ccaatgggcg gagaattcgt tgaaggtttc ccacgcatcg atccaacgtt tgttggcatt
     1381 ccaaattggt ttgcacaacc cataatacca agccgcgacc ttgcgggcat ccatatagcc
     1441 gttaggcgtc atcggctcaa aggcttgcat atccgcgccg ggtgtgtcgt tgatgcggcc
     1501 gactgtaaaa acattcggca caattttctt ggcttcaaag gcggcgccaa aatcatctac
     1561 gcatttaaca acggccagtg attgcccggc gtcagcgacg cgctgtagaa aatcgccgta
     1621 gccgttgcgc ggacccatga gactgtgcac accaattttg ctgctcatcg ttgtcctctc
     1681 ggccgaatga agatagtctc aatgtgcggc gcgattcccg atcgccattt gctcagtgct
     1741 gcagctcgcg ccgctgcaag cctgtcgtgc tgagcgttga atgagccgaa taactagtgt
     1801 tgatcggcgt gcatctattg aactgcagac tgccgcgcag tgtcgacaca ctgccaaaga
     1861 attgatactg ctggctgaga tcaattggaa cgctgaatgc gatcatcgtt aagaataata
     1921 tacggcagaa acagtgtcgc tgtcaatatg caatgtgaaa aatattttcg acaaatgtca
     1981 tgactaaagt ttgaagtgtg tttgaagatg ttgctgcgca gcttgtcttt aacactcaat
     2041 cttgaattga tgcatataat gcgcaaagaa ttggatcgtt ctcaaatcaa aatcaaacca
     2101 agtggcggct aagcctgctc ttaaacctaa ttacaaccaa actattaatc gcagctgcgg
     2161 gtgaatcatg ttcgcgtgag tttgaataca attcaaaatt tcaggccgga aattttagtg
     2221 gtattggagt acaatccatt ccagttgtgg tcggtgtgcc cgcatgcaaa ttgtttcctt
     2281 atcaaggagt agttcaccat gaaagaccga aagctgctga tgatccccgg cccgatcgaa
     2341 tttgatccgg cggtgctggc ggcgatgagc ctgccgacga cgagccatgt cgccgcgaac
     2401 ttcatcgatg ttttcggtca agcgttggag aaaatgcgca aggtctggct gtcggatgac
     2461 ggccagccga tcgtgatcgc tggctccggt actttggcaa tggacaccgc gggcgcgaac
     2521 ttgatcgagc cgttcgataa agcgttggtg gtgaacaccg ggtacttcag tgatcgattt
     2581 ggatcgatcc tggaaagata tggcgcaacg gtgacgcagc tgcgcgggcc gatcgccggc
     2641 cgacccacga tcgaggaggt ggaagcagcg ctcaagaacg gtcgattcaa agtgatgacc
     2701 gtgacgcatg tcgatacatc gaccgcggtg atcaacgatg tgaaagcgtt ggcggcactg
     2761 gcgcgcaaat atgaaacgct gatcgtcgtc gatggcgtct gttcaatcgc cggtgaagaa
     2821 tttagaatga gcgaatgggg catcgacgtg gcgctgaccg cgtcgcaaaa agcgatcggt
     2881 gttccgccgg gcttggcgct gctcgtcgcc ggaccgcgcg cgatcgaagc gctgcgcaaa
     2941 cgcgaatcgc cggtgatgaa ttactacgct gattggaata actggctccc cgtcttggaa
     3001 gcgtatgagg cgcgcaaggt cggttacttc ggcacgcccg cgatcaattt ggtatgggcg
     3061 ttgaatgtga gtctcgatca gatattaaag gaaggcctgg aggcgcgagt ggagcggcat
     3121 cgcaagttga gtcgatcgat gcaagcggcg atcaaggcgt tgggcttggg tcaagttcca
     3181 ttaaatgaag actgtgccgc gcacacattg agtgcgccgc gttatccgca aggtgtgact
     3241 tcggcggatc tgctgccgcg ggttgaagcg gcgggtgcga tcattgcggg cggtttgcat
     3301 ccgcagatcc gaacggaata cttccgcatc ggtcatatgg gctcgacctc gatcggcgat
     3361 ctgctggcga cgatcggcgc ggtggagatc ggcttggccg gttgcgggta taaattcgaa
     3421 atgggtgccg gtgttgcggc ggcgatcaag agtaataacc acggctgaca tgaatgacac
     3481 tgaca
//
LOCUS       DASRTJ010000057         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_230448,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000057 DASRTJ010000000
VERSION     DASRTJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230448"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFF70_01070"
     CDS             complement(<1..784)
                     /locus_tag="VFF70_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZY43314.1"
                     /translation="MKILILNQSEVEQLLPMRNCIEVMVEALTALAQGRVHLPLRMVV
                     RPTDAKGLLGLMPAYMSGARAAFGIKIIGVFNGNPAIGKDSHQGAVLLISGETGEPLA
                     LMNASAITAIRTGAVSGVATRLLAREAAGDLAIIGSGVQARSHLAAMACVRSIKRVRI
                     VSRSFDHAQRFVSEMRLLYSFPVEAVKSVREAVDHADLIVTVTSSSEPILKREWISSG
                     AHLNVVGASLPHAREVDSATMAASSLFVDRRESTLNESGDYLF"
     gene            complement(905..>1574)
                     /locus_tag="VFF70_01075"
     CDS             complement(905..>1574)
                     /locus_tag="VFF70_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43315.1"
                     /translation="DWIDRSELQINRRAVPAPERSSNAMSVVSCGSPIAGVQVEIVDE
                     HDQPLPDRWVGHIRARSAWMLKEYFNAPEKTAQNIRGGWFYSGDMGYLVDGQLYVCGR
                     QKDVIITYGKNIYPEDLEEIANKIAGLAPGRNVAFGVMNDTQGTEGVVLVCELRPKID
                     PQDLQMIERTLRQQVMQVLNIALADVCLVRERDWVIKTTSGKIARSANREKYLKEFDR
                     RESY"
BASE COUNT          356 a          466 c          389 g          363 t
ORIGIN      
        1 caaacaaata atcgccggat tcattcaagg tcgattcacg tcgatcgaca aacagactcg
       61 acgcggccat cgtcgcggaa tcgacttcgc gcgcgtgcgg cagactcgcg ccgacgacgt
      121 tgagatgcgc gccagatgaa atccattcgc gcttcaagat cggttcgctt gatgaagtaa
      181 ccgtcacaat caagtctgcg tgatcaacgg cctccctcac agattttaca gcctcgacgg
      241 gaaatgaata aagtagcctc atttcagaca cgaagcgttg agcgtgatcg aacgatcggc
      301 tgactatacg cactcgcttg atcgatcgaa cgcaggccat cgccgccaga tgcgatcggg
      361 cttgcacgcc ggagccgatg atcgccaaat cgcccgcagc ttcacgcgcg agcaatcgag
      421 ttgccacgcc cgacacggcc cccgtgcgga tcgccgtaat cgccgaagcg ttcatcaatg
      481 ccagcggctc gccggtttcg ccgctgatca ataagaccgc accttgatgc gaatctttgc
      541 cgatggccgg gttgccgttg aacacgccga tgattttgat gccaaatgca gctcgagcgc
      601 cggacatata cgcgggcatt agaccgagca agcctttggc atcggtcgga cgaacaacca
      661 tgcgcagcgg cagatggact cgaccttgcg cgagtgcagt caatgcttcg accatcacct
      721 cgatacaatt tcgcatcggc agcagttgct caacttctga ttgattgagg atcaaaattt
      781 tcatcgtgac tcgccccgat caaaaacttt cagatacttt tcgctactat gctctcgaac
      841 cgcgtcttcg cggttcggct cgcacgcgag cttagagcaa gctttagaat ttggatcaat
      901 gttttcagta ggactcgcgc cgatcaaatt ctttcaaata cttttcgcgg ttggccgatc
      961 gcgcaatctt gccgctggtg gttttgatca cccaatcgcg ttcgcgcacc aaacacacat
     1021 cggctaaagc gatgttcaac acttgcatca cttgttggcg cagtgtgcgt tcgatcattt
     1081 gtaaatcttg tggatcgatc ttgggccgca gttcacacac cagcaccacg ccttcggtgc
     1141 cttgtgtgtc attcatcacg ccgaaggcca cattgcgtcc cggcgcaagt ccagcgattt
     1201 tgttcgcaat ttcttccaag tcttcgggat aaatattttt gccgtaagtg atgatcacat
     1261 ctttctgccg gccacacaca tacaactgcc cgtcaaccag gtaacccata tcgcccgaat
     1321 aaaaccagcc gcctcgaatg ttctgcgctg ttttctccgg tgcattgaaa tattctttca
     1381 gcatccaggc gctgcgggca cggatgtggc cgacccatcg atcaggcaac ggttgatcgt
     1441 gttcatcgac gatttcaact tgaacaccgg cgatcggtga accgcagctg acaaccgaca
     1501 tcgcgttcga cgatcgctct ggcgcgggca ccgcccgccg attgatctgc agctctgatc
     1561 gatcgatcca atcg
//
LOCUS       DASRTJ010000058         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_79080,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000058 DASRTJ010000000
VERSION     DASRTJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79080"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4022)
                     /locus_tag="VFF70_01080"
     CDS             complement(<1..4022)
                     /locus_tag="VFF70_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43316.1"
                     /translation="MKKKTTTKKRLPATRTTTAPARLRSTSMTKPKPRATAAIDWQSI
                     AEQRAAELAIINSVQEGLAAKLDMQAIYDLVGDEIHKVFFEAQVVDILTYDSTTDLLH
                     PQYVIERGVRYEVKPWPVRGFRKHVISTGQPLLINKDIAQKVVEFDNPEFIMGESTKS
                     WLGVPMLVGREVKGVISLQHIDKENAFSESDVRLLSTLAASMSVALENARLFDEVQKK
                     NVEITESLEQQTATSEVLRVIANSPSDIQPVLDAVAENAARVCGANDALIFQVEGNQL
                     RQVAHYGPIPVQPDELALPINRGSAGGRAVVLGDTIHIHDILAESDSEFPISKAIHNR
                     SKRRTLLATPLMRERVPIGVIAIRRTEVDPFTDKQIALLKTFADQAVIAIENVRLFDE
                     TQRLLKDTQQRAAELAIINSVQQGLASKLDMQAIYDLVGDKIQEIFDAQIVVIGMADS
                     QAGHAYFPYAIEQGTRIELRADPTDALGDYLRSNRQPLLINMERDGVRFGRARGPKSF
                     LTVPMYVGEKFIGAISLQNADRENAFSEADVRLLATLASSMSVALENARLFDETQRLL
                     KETDQRAAELSIINSVQEALASKLDMQGIYDAVGDKVSEIFPNVEVGIRILDPQTKMV
                     HFAYAVERGQRLQIPPMPLQQEVITKHVLNTRLPLLVNENLVEETQQKYGAFTIPGTQ
                     PEKSALWVPLIVGSEGRGLIELVDMDREHAFSDSDVRLLTTVANSMSVALENARLFDE
                     TQRLLKETDQRVAELQIINSIQQGLAAELDFQAIVDLVGDKLREVFNTGDIGLNWFDE
                     KANLVHFLYTYEHGKRLMVPPQPPAQRGIHERLAKTRKSWVLNTAADLQEANIPLIPG
                     TDQCKSMVAVPVISGDRLLGTLQLENHERENAYGESELRLLTTIAASLGTALENARLF
                     DETQRLLKITEDRAAELAIINSVQAALAAELNIQGIYDAVGDKIREIFHQADVGIRIY
                     DPQTNLIHFPYLYENGKPIAIKSDPLPQQGFGPHVIRTRETLVINENMAQAMEKYGSY
                     VIPGTEPLKAGVYVPLVVGDQARALIQIVDTEREHVFSESDVRLLQTLANSMSVALEN
                     ARLFDETQRLFKESEQRAAELAIINSVQAGLASKLDMQSIYDLVGDKIREIFAADTTY
                     IAQYNSEAQIFHFPYYVDKGQRLGNGTLPLGRGLTSAVFNARQPLLLGTQAEQHARGG
                     VTDAYQNAAEDLNQTYLGVPILIGGEVRGVISVQRYRQNSYDETHVRLLETLANSMSV
                     ALENALLFDETQRLLKETDQRAAELSIINSVQQGLASKLDMQSIYDLVGEKIREIFSA
                     DVVGISLYDRSQNLMRIPYML"
     gene            complement(4041..>5191)
                     /locus_tag="VFF70_01085"
     CDS             complement(4041..>5191)
                     /locus_tag="VFF70_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43317.1"
                     /translation="QKSMTLHFTVRDTGLGIPPDRMGRLFQSFSQVDASTARKYGGTG
                     LGLVISKRLSEMMGGNMWAISEGVPGKGAIFHFTIKTQAADAPATSTRRDLRGVQAPL
                     TEKRILIVDDNATNRRILTLQTQKWGMQPRDTASPLQALDWIKHGDPFDVAILDMHMP
                     EMDGLELAQAIRESRDPKALPLIMFTSLGRREGGADSIEFAAHLTKPIKPSQLFDALV
                     SIFVEQPTLVRKAETIKTQFDPEMAHKHPLRILLAEDNAVNQKLALRLLQQMGYRADV
                     AGNGLEAIEAVERQKYDVILMDVQMPEMDGLDATRAIVARWPKGVRPQIIAMTANAMQ
                     GDREMCLEAGMDDYMTKPIRIDELVAALLRSKPTIDQPQLPLSDADKK"
BASE COUNT         1064 a         1457 c         1395 g         1275 t
ORIGIN      
        1 agcatgtaag ggatgcgcat caaattttgg ctgcgatcat acaagctgat gccgacgaca
       61 tcggctgaaa aaatctcgcg aatcttctcg ccgaccagat cgtagatcga ttgcatgtcc
      121 aacttggagg cgaggccttg ctgcacgctg ttgatgatcg atagttcggc ggcgcgttga
      181 tcggtttctt tgagcagccg ctgcgtttca tcaaacagaa gggcgttttc gagcgccacg
      241 ctcatcgagt tggcaagggt ctccaacagg cggacatggg tttcatcata agaattctgc
      301 ctgtagcgct gtacgctaat gactcctcta acttcgcccc cgatcaagat ggggacgcca
      361 aggtaagtct ggtttagatc ttctgcagca ttttgatacg catcagtaac gccgcctcgc
      421 gcgtgctgct ctgcttgtgt acctaagagg agcggctgac gtgcgttaaa gacagctgac
      481 gttagccctc gtcctaatgg aagagtcccg tttcctagtc gctggccttt gtcgacatag
      541 taagggaaat ggaaaatttg cgcctcggaa ttatattgtg caatatatgt tgtatctgcc
      601 gcgaaaatct ctctgatctt atccccgacc agatcgtaga tcgattgcat gtccaacttg
      661 gaggcgagac cggcttgcac gctgttgatg atcgccagtt cggcagcgcg ctgttcgctt
      721 tctttgaaca ggcgctgcgt ttcgtcgaac aggcgcgcat tctcgagtgc cacgctcata
      781 ctattggcga gcgtttgtag caggcgcaca tcggattcgc tgaagacgtg ttcgcgctcc
      841 gtatcaacga tctgaatcaa ggcgcgggcc tgatcaccga cgacaagcgg cacataaacc
      901 cccgctttca gcggctcggt gccgggaata acgtaagagc catacttctc catcgcttgc
      961 gccatgtttt cgttgatcac caatgtctca cgggtacgaa tcacatgagg accaaagccc
     1021 tgctgaggca gcggatcaga tttaatggcg atgggcttgc cgttttcgta cagatacggg
     1081 aagtgaatca aattcgtctg cggatcatag atgcggatgc ccacatccgc ctgatggaag
     1141 atttcgcgga tcttgtcgcc gactgcgtcg tagatgcctt gaatgttcag ttcagcggca
     1201 agcgcggcct gcacgctatt gatgatcgcc agttccgcgg cgcggtcttc ggtgatcttg
     1261 agcaagcgtt gtgtctcatc gaatagccgc gcgttctcga gcgcggtgcc caacgacgca
     1321 gcgatggtgg tcagcaggcg cagttcggat tcaccgtagg cattttcgcg ttcgtgattt
     1381 tcgagttgta acgtgccgag caggcggtca ccactgatga ctggcaccgc gaccattgat
     1441 ttgcattggt cggtgccagg aatcagcggg atgttcgcct cttgcaaatc tgcggcggtg
     1501 ttcaataccc acgatttgcg cgttttggcc agtctttcat ggattccacg ctgggcgggt
     1561 ggttgaggcg gaaccattaa gcgttttcca tgctcatagg tgtaaagaaa gtgaaccaga
     1621 ttggctttct cgtcaaacca attcaacccg atatcgcctg tgttgaatac ttcccgtagt
     1681 ttatcaccga ccagatccac aatggcctgg aaatccaact cggcggccag accctgctgg
     1741 atcgaattga tgatctgcaa ttcggcaacg cgctgatcgg tttctttcag cagccgttgg
     1801 gtctcatcga acaggcgcgc gttttccagc gcgacgctca tactattggc gaccgttgtc
     1861 agcaagcgca catctgaatc gctgaaggcg tgctcgcgat ccatatccac caattcgatc
     1921 aggccccggc cttcactgcc gacgatcagg ggaacccata gcgccgattt ctcaggctga
     1981 gtgccgggaa tggtaaaggc tccgtacttt tgctgcgttt cctccactaa attttcattg
     2041 acgagcagcg gcagacgggt attgagaacg tgcttagtaa tcacttcttg ttgaagtggc
     2101 atgggcggga tttgcagccg ctggcctcgc tccaccgcat aggcaaaatg gaccatcttc
     2161 gtctggggat ccaagatgcg aatacccacc tcgacattgg ggaaaatttc gctgaccttg
     2221 tcgcctactg catcataaat accttgcatg tcaagctttg aggctaatgc ttcctgcacg
     2281 ctgttgatga tcgatagttc ggcggcgcgt tgatcggttt ctttcaacag gcgctgggtt
     2341 tcatcgaaca agcgcgcgtt ttccagcgca acactcatcg atgacgccag tgttgccagc
     2401 aaacgcacat cggcttcgct gaaagcattt tcgcgatcgg cattttgcag cgaaatcgcg
     2461 ccaataaatt tttcgccgac atacatcgga acagtcaaga aagacttggg accccgcgcc
     2521 cgtccgaacc tgacgccgtc acgttccata ttaatgagca acggctggcg attactccgc
     2581 aagtaatctc ctagtgcgtc agtcggatcg gctcttagct cgatacgcgt accttgttca
     2641 atcgcatacg ggaaatatgc gtggcctgcc tggctgtctg ccatccctat aaccacgatc
     2701 tgcgcatcaa aaatctcttg aatcttgtcg ccgaccagat cgtagatcgc ctgcatgtct
     2761 aacttcgacg cgagaccttg ctgcacgctg ttgatgatcg ccagctcggc ggcgcgctgt
     2821 tgagtgtctt tgagcaagcg ctgggtctca tcgaacagcc gcacattctc gatcgcaatc
     2881 acggcttgat cggcaaaggt cttcagcagc gcaatctgct tgtcggtgaa cggatcgacc
     2941 tccgtgcggc gaatcgcaat cactccaatc ggaacgcgct cgcgcattaa gggtgtggcg
     3001 agcagcgtcc gacgtttcga tctgttatgg atcgccttgc tgatcggaaa ttcagaatcg
     3061 ctctcggcca gaatatcgtg gatgtggatc gtgtcgccca gcacaacggc gcgcccgccc
     3121 gcagaaccgc ggttaatagg cagagctaac tcatccggtt gtaccggtat tggaccataa
     3181 tgagcaacct gacgtagctg gttaccctcc acctggaaaa tcagcgcatc gttggctccg
     3241 cacacgcggg ctgcattttc cgcgaccgca tccagaacgg gttggatgtc gcttggagaa
     3301 ttggcaatga cccggagcac ctcgctggtg gcggtctgct gttcaagcga ctcggtgatt
     3361 tcaacattct tcttctgaac ttcgtcgaac aggcgcgcgt tttcaagcgc gacgctcatg
     3421 gatgccgcga gcgtgctgag taagcgcaca tccgattcgc tgaaggcgtt ttccttgtcg
     3481 atatgctgta ggctaatgac gccttttact tcacggccca ccaacatggg gacacccaac
     3541 cacgatttgg ttgattcacc catgataaat tcagggttat caaattcaac taccttctgt
     3601 gcaatatcct tgttgatgag gagcggctgt ccggttgaga tgacatgctt tctgaagccg
     3661 cgcacgggcc agggttttac ttcgtatcgg acacctctct caatgacata ttgaggatgg
     3721 aggagatcgg tcgtagaatc gtatgtcagg atatccacaa cctgcgcctc aaagaaaacc
     3781 ttatgaatct cgtcgccaac cagatcgtag atcgcttgca tgtccaactt ggcggccaga
     3841 ccttcttgca cgctgttgat gattgccagc tcggcggcgc gctgctcggc gatcgattgc
     3901 caatctatgg ctgcagtcgc gcggggcttc ggcttcgtca tcgaagttga tcgcaatcga
     3961 gccggggctg tggtggtgcg tgtggctggt aacctttttt tagtcgttgt ctttttcttc
     4021 atagcttatt ttccctgcga ttactttttg tctgcgtcac tcaacggcag ctgcggctga
     4081 tcgattgtcg gtttcgatct cagcagcgcc gcgaccagtt cgtcgatgcg gatcggcttg
     4141 gtcatgtaat cgtccatgcc cgcctcgagg cacatttccc gatcgccctg catcgcattc
     4201 gcggtcatcg ctattatttg cggccgcacg cctttcggcc agcgcgccac gatcgcgcgc
     4261 gtggcatcca atccatccat ctccggcatc tgcacgtcca tcaagatcac atcgtacttc
     4321 tggcgctcca ccgcctcgat cgcttccagt ccgttgccgg ccacatcggc gcgatagccc
     4381 atctgctgca acagccgcag cgcgagtttt tgattgaccg cattgtcttc ggccagcaaa
     4441 attcgcagcg gatgtttgtg cgccatctca ggatcgaact gcgtcttgat cgtttccgct
     4501 ttccgcacga gcgtcggctg ttcgacgaaa atactgacga gcgcatcgaa taactgggac
     4561 ggcttgatcg gtttggtgag atgcgcggca aattcgatcg aatctgcacc gccttcgcgt
     4621 cggcccaacg acgtgaacat gatcaatggc agcgcttttg gatcacgtga ttcgcgaatc
     4681 gcttgcgcca actccagacc atccatctcc ggcatgtgca tatcgaggat cgccacatcg
     4741 aacggatcac cgtgtttgat ccagtccagc gcctgcagcg gcgacgccgt atcgcgcggc
     4801 tgcatgcccc acttctgcgt ctgcaaagtc aggatgcgcc gattggtcgc gttgtcgtcg
     4861 acgatcaaga tgcgcttctc ggtcagcggc gcttgcacgc cgcgcagatc gcggcgagtg
     4921 gatgtagcgg gcgcatcggc ggcctgcgtc ttgatcgtga agtgaaagat cgcgcctttg
     4981 cccggcacgc cttcgctgat cgcccacata ttgccaccca tcatttcact caggcgcttg
     5041 ctgatgacca gacccaaacc cgtgccgcca tacttgcgcg ccgttgacgc atccacttga
     5101 ctgaacgatt ggaagagccg ccccatacga tcgggcggaa tgccgagacc ggtatctctg
     5161 accgtgaagt gcagcgtcat tgatttctga t
//
LOCUS       DASRTJ010000059         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_342193,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000059 DASRTJ010000000
VERSION     DASRTJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342193"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1693
                     /locus_tag="VFF70_01090"
     CDS             41..1693
                     /locus_tag="VFF70_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZY43318.1"
                     /translation="MKHYEESVPFTISLPDRPVHRFLRSAARRFPNRIAIKFYGRSIT
                     YRALSAEADRFANALRDLGVQRSDRVMILLPNVPQTVIAYYGILRIGGVVVFTSPTNS
                     AEEIIRQVKDSGAKVLIGLGRFAAMIDRVQAQARLDHVILTYVRDYLPSIKKRGLRIQ
                     HPLRHGNRSGAQQWSEIIKNQKAIRPDVHIGRNELAVIIYTGGTSDRPKGVMLSHGNL
                     VANALQTRHWITDIREGHETFLCALPFSHSYGLSATLNAPVMIGATMVLLPTFITEDV
                     LKTIKRERPTLFPGVPTMYMAINNFRGVRKFGIRSIRACISGAAPLPVEVQEAFEKLT
                     RGRLVEGYGLTEASPITHGNPLNGLRKIGSIGIPFPSTEAKIVDIATGQDLPAGHTGE
                     LVVRGPQVMLGYWDLPEETDRVLHKANDDRGLWLYTDDIATMDEDGYFTIINRKKDMI
                     LAGEYQVYPRDVEEVLFEHPKVKDVVVLGVPLTPVGQKVKAFVVLREGEKATPDELLA
                     MCRARLAEWEVPWEIEFRNELPKSFVGKVLRRVLLEEEKLNA"
     gene            1761..2228
                     /gene="greA"
                     /locus_tag="VFF70_01095"
     CDS             1761..2228
                     /gene="greA"
                     /locus_tag="VFF70_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZY43319.1"
                     /translation="MEPAPTYLTPEGKKKLEEELEILKTTKRKELADRLNFAIKQGDL
                     SENADYIMAKEDQAFLEGRIRTIEVMMRDVVLIKEESGGAVRIGSRITVKEVGGDDDE
                     QFNIVGAAEADPMNGKISNESPLGKQLLGKSIGDQVEIDAPGGKIKFKIVAID"
BASE COUNT          612 a          598 c          692 g          506 t
ORIGIN      
        1 cgagacggcc agaacaaaat tgatcaggga gcgcccgtgg ttgaagcatt atgaagagag
       61 cgtgcctttc acgatcagtt tgccggatcg acctgtgcat cgattcctgc gcagcgcggc
      121 gcgccgcttc ccaaatcgaa tcgcgatcaa attctacgga cgatcgatta cgtatcgagc
      181 attgagcgcg gaagccgatc ggtttgccaa cgccctgcgc gatctcggcg tgcagcgcag
      241 tgatcgcgtg atgatcctgc tgccaaacgt tccacagacc gttatcgcct actacggcat
      301 cttgcgcatc ggcggggtcg tcgtgttcac cagcccgacg aacagtgcag aagaaattat
      361 tcgacaggtc aaagattcgg gcgcgaaagt cttgatcggt ctcggtcgtt tcgccgcaat
      421 gatcgatcgg gttcaagctc aagcccgatt ggatcacgtc atcctcacgt acgttcgaga
      481 ttatctgcca tcgatcaaaa agcgcgggct tcgaattcaa catcccctgc ggcatggaaa
      541 tcgatcgggc gcgcagcaat ggtcggaaat catcaagaat caaaaagcga tccggcccga
      601 cgtgcatatc ggacgaaacg aattggccgt gatcatttat accggcggca cgagcgatcg
      661 accgaaaggc gtgatgttgt cgcatggaaa tttagtggcg aatgctttac agacacggca
      721 ttggatcacc gacattcgcg aagggcacga aacattttta tgcgcgctgc cgttctcgca
      781 tagttatgga ctaagcgcga cgttgaatgc gccggtgatg atcggcgcga cgatggtctt
      841 gctgccgacg tttatcactg aagatgtgtt gaagacaatc aagcgcgaga ggccgacgct
      901 gttccccggc gtgccgacga tgtatatggc gatcaataat tttcgcggcg tgcgaaagtt
      961 tggcatccga tcgatccgcg cgtgcatcag cggcgcggcc ccgctgcccg ttgaagtaca
     1021 agaggcattt gaaaaattga cgcgcggtcg attagtagaa ggttatggct tgaccgaagc
     1081 gagtccgatc acgcacggca atccgttgaa cggcctgcgc aagatcggat cgatcggcat
     1141 tccgttccca tcgaccgaag cgaagatcgt cgatattgcc accgggcaag atttgcccgc
     1201 cggtcacacg ggcgaactgg tcgtgcgcgg cccgcaagtg atgctgggct actgggactt
     1261 gccagaagaa accgatcgcg tgttgcacaa agccaatgac gatcgaggcc tgtggttgta
     1321 tacggacgac atcgcgacga tggatgaaga cggttacttt acgatcatca accgcaagaa
     1381 ggatatgatt ctggcgggcg aatatcaagt ttatccgcgc gatgtggaag aggttttgtt
     1441 tgagcatccc aaagtcaagg atgtcgtggt gttgggtgtg ccattgactc ccgtgggaca
     1501 aaaggtcaaa gcgttcgtcg tcctgcgcga aggcgagaag gccacgcccg acgaattgtt
     1561 ggcgatgtgc cgcgcgcggc tggccgaatg ggaagtgccg tgggagatcg agtttcgcaa
     1621 tgaactaccg aagagttttg tcgggaaagt gctgagaaga gtgttgcttg aagaagaaaa
     1681 attaaacgcc taattcacaa tgcacattga tcaattatgc attatacttt gtgcattaag
     1741 cattcgccga ggagacttaa atggaacccg ccccaactta tttaacacct gagggcaaaa
     1801 agaaactcga agaggaattg gaaatcctca agacgaccaa acgcaaagaa ttggccgatc
     1861 gattgaactt tgcgatcaag caaggcgact tgtccgagaa cgcggattac atcatggcga
     1921 aagaggatca agcctttctg gaaggccgca tccgtacgat cgaagtgatg atgcgcgatg
     1981 tcgtcctgat caaagaggag agcggcgggg cagtgcggat cggatcgcgc attaccgtca
     2041 aggaagtcgg cggcgatgac gatgagcaat tcaacatcgt cggcgcggcg gaagccgatc
     2101 cgatgaacgg caagatcagc aacgaaagtc cgctgggcaa gcagctgtta ggcaaatcga
     2161 tcggcgatca agtcgagatc gatgcgcccg gcggcaagat caagttcaag attgtagcga
     2221 ttgattaatg tgcctccagg tcattgcgag cgtagtccct agatggcaca accctctagg
     2281 tctgtgccat gaagcaatct caatagccat caaagagatt gcttcgtcgc gctaagcgct
     2341 cctcgcaatg acgccttcaa ttttcgctgt cacagctata aggaatttaa aacatgaccc
     2401 cactagat
//
LOCUS       DASRTJ010000060         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_266492,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000060 DASRTJ010000000
VERSION     DASRTJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266492"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFF70_01100"
     CDS             <1..202
                     /locus_tag="VFF70_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HZY43320.1"
                     /translation="RSIRAAGAQELIAAVPVGPNQVVRRLRHEADRVVCLAMPNNFWA
                     VGQYYERFGQVSDEEVVGAMTN"
     gene            complement(237..1562)
                     /locus_tag="VFF70_01105"
     CDS             complement(237..1562)
                     /locus_tag="VFF70_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43321.1"
                     /translation="METAPILLAYLLPIGALLVAWGGWTDDRSRENAASALLIIAIAV
                     IAYAAIGFAFQFGGVGLRPDAPIGLRGLDRMWSPVSTANNPWGVIGLEGFLLNAQSTA
                     SGDTALLFALFLHQLPIVITTTLIPALTLAGRVRNFTLVIIASITATIIVPIASMWSW
                     GNGWLSTLGSAVNFGHGFIDPGGASAAFFAAGMITLASLIALKIRRISTEPIELPHLI
                     APLRSIFGAIVLGLGWLAWLLTDPFLQANRSIDLSFAASNILLGAAAATLFSFAYSWF
                     TTGKPNALLTSRSAMGGFIALAASAAFVPTWAAIVIGGIAGLLTPIAIFVIDRLQLDD
                     SSGSIATAGLAGLWSLISIGFLADGTYGAGWNNVGVKEYLGAAGQGVTGLLAKANLQN
                     DPGQMSAQITGLLAIGLFAFVMSWLFIRPWRRLTKIHTLEHSLMPTDDE"
     gene            1710..>2876
                     /locus_tag="VFF70_01110"
     CDS             1710..>2876
                     /locus_tag="VFF70_01110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY43322.1"
                     /translation="MTFSDGENVGPYQIMGQLGSGGMATVYKAYHAALDRYVAIKVLH
                     PAFKQDANFLSRFQREARIVAKLEHPHIVPVYDFNEHAGQPYLVMRFIDGETLKARLV
                     QGDLPFSEIVRILNPTGDALAYAHAQGVLHRDVKPSNILLTPDSGVFLADFGLARIAQ
                     AGESTLSQDSLLGTPQYISPEQAQGRSDLDVRTDVYSLGVVMYELLLGRVPYQADTPY
                     AVIHDHIYTPLPLPRSIKPALSESLERVLLKALAKDREDRFSSVNELILAFDQGMQSA
                     PSEVEPSASIEPAKLIRPERTASTIAAPPVSTPIVQSTAAAPNRSKLGIGLGAAAILI
                     ALIVIVGLAASRPPLPVPPSQLPVGNPSPVNSSAQAQIDQLVQQFNQANQQASKG"
BASE COUNT          682 a          781 c          807 g          606 t
ORIGIN      
        1 gcgttcgatt cgagcggcgg gtgcgcagga gttgatcgcc gcagtgccgg tggggccgaa
       61 tcaagtggtg cgccgcttgc ggcacgaagc ggatcgcgtg gtgtgcttag cgatgccgaa
      121 caatttttgg gcagtgggcc agtattacga gcgctttggg caggtcagtg atgaggaagt
      181 ggttggagca atgactaatt aaaaacgaca aatgagcaaa tgaataatac tcgcgatcat
      241 tcgtcgtctg tcggcatcag actgtgttca agtgtgtgta tcttcgttag tcttcgccaa
      301 ggtcggatga ataaccacga catcacaaat gcaaataaac cgatggccag aaggccagtg
      361 atctgcgcgg acatctgacc gggatcgttc tgcaagttcg ctttcgctaa caggccggta
      421 accccttgtc ccgctgcgcc gagatactct ttcacgccca cattgttcca acccgcaccg
      481 taagttccat cggccaaaaa accgatcgag atcagtgacc agagaccggc cagtccggct
      541 gtggcgatcg agccggatga atcgtcgagc tgcaatcgat cgatcacaaa tatggcgatc
      601 ggcgtcaaca agcccgcaat gccgccaatg acgatggcgg cccacgtcgg cacaaacgca
      661 gcagatgcgg cgagcgcgat gaagccgccc attgcgctgc gtgaagtgag cagcgcattc
      721 ggtttgcccg tagtgaacca actgtaagcg aatgaaaaca aagttgcagc agccgcaccc
      781 aataaaatat tgctggccgc gaaagacaga tcgatcgatc ggttggcttg caaaaacgga
      841 tcggtgagca gccaggccaa ccagccgagg cctaacacga tcgcaccgaa gatcgatcgg
      901 agaggtgcaa tcaagtgagg cagttcgatc ggttccgttg atattcggcg tatcttcaat
      961 gcgatcaagc ttgccagtgt gatcattccg gccgcgaaga aggccgcgct cgcgccgcct
     1021 ggatcgatga agccatgtcc aaagtttacg gcgctgccga gagttgatag ccagccgtta
     1081 ccccacgacc acatactcgc gatcggtacg atgatagttg ccgtgatgct ggcgatgatg
     1141 acgagcgtaa aattgcgcac gcgcccggcg agcgtcagtg cgggaatgag cgtggtggta
     1201 atgacgattg gcagttgatg cagaaacaac gcgaagagca gggcggtatc gccgctggca
     1261 gtcgattgcg cgttgagcag aaatccttcc agcccgatca cgccccatgg attattggcc
     1321 gtgctgaccg gcgaccacat tcgatcgagg ccgcgtaaac cgatcggggc atccggccgc
     1381 aaacccacgc cgccgaattg aaacgcaaat ccgattgcag cgtacgcgat cacggcgatg
     1441 gcgatgatca gcaaagcgct ggcggcattc tcgcgggatc gatcgtcggt ccagccgccc
     1501 cacgcgacca ggagcgcgcc gatcggcagc aggtaggcta gaaggattgg tgcagtttcc
     1561 attcgagatc aattctcaat gcggagtgca caatacatga tactctcatg aagagagcgc
     1621 aataatacca cgattggtca tcgtgcattt agccgtgtgc attgtgaatt gcatatttgc
     1681 agactataat tgcgcgcacg aggcacacga tgaccttttc agacggcgag aatgtcgggc
     1741 cgtatcaaat catggggcaa ttgggcagcg gcggcatggc gacggtctac aaagcctatc
     1801 acgccgcgct cgatcgctac gtggcgatta aagtactgca tccagctttc aagcaagatg
     1861 ccaacttcct ctcacgcttt cagcgcgaag cacgcatcgt ggcgaaattg gaacatccgc
     1921 acattgtgcc cgtgtacgat ttcaatgagc atgctggtca accgtatctg gttatgcgtt
     1981 tcatcgatgg agaaactttg aaggcgcgtt tggttcaagg cgatctgcca ttcagtgaga
     2041 ttgtgcgtat cttaaatccg actggcgacg cattggccta cgcgcacgcg caaggcgtat
     2101 tgcatcgcga tgtaaagccc agcaatattt tgctcacacc cgatagcggc gtgttcctgg
     2161 ccgattttgg tttggcgcgc atcgcgcaag cgggcgaatc gactttgtcg caggattctt
     2221 tactcggcac gccgcaatac atttcgcccg aacaggctca aggccgatcg gatctcgacg
     2281 tccgcacgga cgtgtattca ttaggtgtgg tgatgtatga attgctcctt ggccgcgtcc
     2341 cgtatcaggc tgatactccg tatgcagtga tccatgatca catctacacg ccgcttccac
     2401 tgccgcgctc gatcaagcca gcattgtccg aatccttaga gcgcgtcctg ctcaaagctt
     2461 tggcgaagga tcgcgaagat cgatttagca gcgttaatga attgatattg gcatttgatc
     2521 aaggaatgca atctgctcca tcggaagttg agccttcggc gtcgatcgag ccagctaaac
     2581 ttattcggcc agagcggacc gcatccacta tcgccgcgcc gcctgtatcg accccaatag
     2641 ttcaatcaac agcggcagca ccgaatcgat cgaaactagg gatcggcttg ggcgccgctg
     2701 cgatcttaat cgcgttgatc gtaatcgtcg gtctcgcagc ctctcgacct ccactgccgg
     2761 tgccacccag tcaactgccg gttggaaatc ctagtccggt taattcatcc gcccaagcgc
     2821 agatcgatca attggtgcag caattcaatc aagcgaatca gcaagcatcc aaaggt
//
LOCUS       DASRTJ010000061         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_266497,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000061 DASRTJ010000000
VERSION     DASRTJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266497"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFF70_01115"
     CDS             <1..313
                     /locus_tag="VFF70_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43323.1"
                     /translation="RPCSYNTSFKTKVSQMTNDPKLDTQEQELSKSYERSEWRSGQAQ
                     QKKLQHYQVDAAAMLESDGLISISLPKEDLKAIRQKAAESGTSYQQLIANIVHQFVTG
                     H"
     gene            complement(396..1772)
                     /gene="radA"
                     /locus_tag="VFF70_01120"
     CDS             complement(396..1772)
                     /gene="radA"
                     /locus_tag="VFF70_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZY43324.1"
                     /translation="MPKTKTITQFVCQQCGGTQLKWMGRCPDCGEWNSFVEVVETKAG
                     AKERSSSVLPRAVPTRLKDVKTDGLDRLPMAMQEFSRVLGGGIVPGCLVLVSGEPGIG
                     KSTLLMQVATQMADTHGPVLYVSGEESTQQIKMRAERLGSGGESLYLLTETDLDEILL
                     HIEQLKPRLAIIDSIQTVYVNELSSGPGSISQVRECAARLQAVAKSTGTSIFLVGHVT
                     KSGSIAGPKVLEHIVDTVLYLEGDRFHAYRLLRSTKNRFGATNEVGVFEMRGNGMAEV
                     LNPSEMFLAERLPNAPGSSIAVTMEGTRPLLVEVQALCSTSAFGLPRRTANGLDINRL
                     LLITAVLSKRIGLKLGDQDVFANVIGGLHINEPAADLAAACSIASSMRDVPVAPDLAI
                     IGEVGLSGELRTVSQLPARLSEAAKLGFKRVLIPKTFRLTDAPADKIEILMARSVGEA
                     LSIAMGKQ"
     gene            complement(1921..>2690)
                     /locus_tag="VFF70_01125"
     CDS             complement(1921..>2690)
                     /locus_tag="VFF70_01125"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY43325.1"
                     /translation="VRFSKSAEGPLGFQAARRYALRALPHVSNRVAPIEVCHIVEPPI
                     LEWLQSNETTNLQINSIDDQVDVRLSNLQLPTSKFQFHVSPDSFFQVNSSLINTLVEQ
                     MLTKLDPKKHQVILDAYCGVGLFTCFIAPKADQVIGIESSRSAIRDAKQNLSEFKNVK
                     LLRGLVEKILPTITGPIDGVVVDPPRAGCGRTVIQALIDHRVQRIVYVSCDPSTLARD
                     ARQLIDGGYQLIEVQPIDLFPQTFHIETVALFVHIGK"
BASE COUNT          654 a          759 c          670 g          607 t
ORIGIN      
        1 ccggccgtgt tcgtataaca cttccttcaa aacaaaggta agccagatga ccaacgatcc
       61 caaactagat actcaagaac aggaactatc aaagtcatac gaacgcagcg agtggcgatc
      121 gggtcaagcg cagcaaaaga aattgcaaca ctatcaagtg gatgcagcgg cgatgcttga
      181 gtcggatggg ttgatcagca tctcgctccc gaaagaagac ttgaaagcaa ttcggcagaa
      241 ggccgccgag tcaggcacat cttatcagca actcattgcg aatatcgtgc atcaattcgt
      301 aacaggccac taaagatcaa aaagggcgag cacgaagctc gccctatcat tttctccaac
      361 aaccaaatcc tgatccccga tccctgatta actgatcact gttttcccat cgcgatcgac
      421 agtgcctcac ccacgctgcg cgccatcaga atttcgatct tgtccgcggg cgcgtccgtc
      481 aagcgaaacg tttttggaat caacacgcgc ttgaagccca acttcgccgc ttcgctcaat
      541 cgagcgggca gctgactcac ggtgcgcagt tcgccgctca accccacttc gccgataatc
      601 gccagatcgg gggcgacggg cacatcgcgc atcgacgacg cgatcgagca cgcggcggcc
      661 agatcggccg caggttcgtt gatgtgcagt ccgccaatca cattcgcaaa cacatcttga
      721 tcgcccaact tcaagccgat gcgtttgctc aacacagcgg tgatcaacag caagcgattg
      781 atgtctaatc cattcgcggt gcggcgcggc aaaccgaagg cgctggtgct gcacaacgct
      841 tgcacttcga ctaacaacgg tcgcgtgcct tccatcgtca cggcgatcga tgagccgggt
      901 gcattcggca agcgctcagc caaaaacatt tcacttggat tcaacacttc ggccatgccg
      961 ttgccgcgca tttcaaacac gccgacttcg ttcgtcgcgc cgaatcgatt cttggttgaa
     1021 cgcagcaagc gatacgcgtg gaatcgatcg ccttccagat aaagcacagt gtcaacaatg
     1081 tgttccagaa cctttggccc ggcgatcgag cccgatttag tcacatgccc gacgagaaag
     1141 atgctggtgc cggtcgattt cgcgacggct tgcagacgtg ccgcgcactc acgcacttgc
     1201 gagatcgatc cgggtccgga actgagttcg ttcacgtaca ccgtctggat cgaatcaatg
     1261 atcgccagtc gcggtttcag ttgctcgata tgcagcagaa tctcatccag atcggtttcg
     1321 gtcagcaaat acaatgactc gccaccgctg cccaatcgtt cggcacgcat cttaatttgc
     1381 tgcgtgctct cttcgcccga cacatacaat accggaccat gtgtgtcggc catttgcgtg
     1441 gcaacctgca tgagcaaagt gcttttgccg atccccggct caccgctgac taaaactaaa
     1501 cagcccggca caatgccgcc gcccagcacg cggctgaatt cctgcatcgc cattggcaat
     1561 cgatcgaggc cgtcggtctt gacgtccttc aagcgtgtgg gcacggcgcg cggcagcacc
     1621 gaactcgatc gttccttcgc gcccgctttg gtctcgacga cttcgacgaa tgaattccat
     1681 tcgccgcaat cggggcagcg gcccatccat ttgagttgag tgccgccgca ttgctggcag
     1741 acaaattgtg tgatggtttt tgtttttggc atttttgcaa tctctgaatc acaatctctg
     1801 atttgtgagc aatccgcgaa gttgtataag attattcata tcaatctgtg accattcgtg
     1861 gacaaccttt tctattatcg cataaatcaa agtcgttgac acaaacttta atttgttgtt
     1921 ctactttccg atatgaacga acagagccac tgtctcaata tgaaatgtct gtggaaatag
     1981 atcgatcggc tggacttcga tcagttgata accgccatcg atcaactgcc gcgcatcccg
     2041 cgctaacgtc gatggatcgc agctgacgta gacgatgcgc tgaacccgat gatcgatcag
     2101 cgcctgaatg accgtccgcc cgcagcccgc ccgcggcgga tcgacaacga cgccatcgat
     2161 cggcccggtg atggtcggca aaattttctc aactaaacca cgcagcagtt taacattctt
     2221 gaattcactc aggttctgtt tcgcatctcg aatcgcgctg cggctcgatt cgatcccgat
     2281 aacttgatcg gccttcggcg cgatgaagca cgtaaacaaa cccacaccgc agtaagcatc
     2341 gaggatcact tgatgtttct tcggatcaag tttagtcagc atctgctcga cgagcgtatt
     2401 gatcaaggac gagttgactt gaaagaaact gtcgggggaa acgtggaatt ggaacttgga
     2461 ggttggaagt tgaagattgg agagacgcac atcaacttga tcatcaatcg agttgatctg
     2521 aagatttgtt gtctcattcg attgcagcca ttccaatata ggaggctcaa caatatggca
     2581 cacctcgatc ggggcgacgc ggttcgacac atgcggtaaa gcacgcagtg cgtaccgtcg
     2641 agcggcttga aaaccgagtg ggccttcagc ggatttgctg aatcgaacgt
//
LOCUS       DASRTJ010000062         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_230647,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000062 DASRTJ010000000
VERSION     DASRTJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230647"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1008)
                     /locus_tag="VFF70_01130"
     CDS             complement(19..1008)
                     /locus_tag="VFF70_01130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43326.1"
                     /translation="MVAHATQPTATFSGARPINLSRDLVSIANLIDRAFADDMDDSGR
                     AAVREMRLLGRLAFLFSWAESFVPQGEGWAPGFVWIEEGDVVANATVRRLSLFGRGWM
                     IGNVAVVPKLRGRGIARSLMNACIDLARQRNGDWIALQVRSNNAIARGLYSSLNFQTI
                     SETIELIRTEPDRVTPPDQSSDGHLRIAEPRDTDKIFALAQAANTESMRWIEPQYRTQ
                     FELGFDRRLANFLSGTFATWRVIEVDKDIVGASSIKVNRWTGASRLGLWVTPAYRGRV
                     EQTLVDAVLSELPASTRSISARIVGDHTAGRDALLSRSFTIVRALTNMRLDLH"
     gene            complement(1199..2290)
                     /locus_tag="VFF70_01135"
     CDS             complement(1199..2290)
                     /locus_tag="VFF70_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZY43327.1"
                     /translation="MTTTQYETSPTPHITISCHDDLSLIGSSDQRVLIEIDDDSPASK
                     IDRSGETITINALDDCEIMCPLNSTVTIQLVSGDLRVIQLTGALAIDTVNGDATLREI
                     GPLMIKTVQGDLELRDVEGEARIETVRGDVKVKHVAGPLSINRVSGDLVAEDLGAGAS
                     FGKIDGDLKIETELRPNQNYNANANGDIVLVVLGGGAKMNLASKGELRSRVPMTEWQG
                     NDRAATGVLGDGSAIVNLSARGDVLILSGDSQWGVDGISDHVESMIESAMDRFETEMT
                     RVQRQLEERFGSQADRLHHRQTDRAKKRTERAAGSWGSFFHSPRAGSTPAPAVEPVTD
                     QERMAILKMVEEKKITSDEAAKLLAALEG"
     gene            complement(2478..>3098)
                     /locus_tag="VFF70_01140"
     CDS             complement(2478..>3098)
                     /locus_tag="VFF70_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43328.1"
                     /translation="FILLGTALSNFINSVFWGMGYSLKQDMDVGVLESSWLAPVPRVL
                     LLVGHTITNLIITTATSLIMLLLAGVIFGFRPSGNALAAFLTMLPMLLGLYGFGFAFA
                     ALVLILREANTLVDVSSFLVQIFSGADFPVNSMPRWLLPIALALPLTYGFDAVRGWLL
                     GTQTILPLGVEVILLIVFMFVMIGLGLIAFRALEVRVRRRGTLGQY"
BASE COUNT          708 a          884 c          823 g          683 t
ORIGIN      
        1 actcagaatg acatacgttt agtgcaaatc caatcgcata ttcgtcaacg ctcgcacgat
       61 tgtgaagctg cgtgataaca gcgcatcccg tccggctgtg tgatcaccca cgatgcgcgc
      121 tgagatcgat cgggttgatg caggcagttc gctcaagacc gcatcgacta aagtttgctc
      181 cactcgcccg cgataggctg gtgtaaccca cagacctaat cgacttgcac ccgtccagcg
      241 atttactttg atcgacgacg cgccgacgat atctttatcg acttcaatca cgcgccacgt
      301 ggcaaaagtg ccgctcaaga aatttgccag gcgtcgatcg aatcccagtt caaattgtgt
      361 gcgatattgc ggctcgatcc agcgcatcga ttcggtattc gcggcttggg ccagcgcaaa
      421 gatcttatcg gtgtcacgcg gttcggcgat tcgcaaatgg ccgtcgctgg actgatcggg
      481 cggcgtaacc cgatcgggtt cagtccgaat caactcaata gtttcgctga tcgtttgaaa
      541 gttcagcgag ctatatagtc cgcgcgcaat cgcattattg gatcgaactt gcaaggcgat
      601 ccagtcgccg ttgcgctgac gggctagatc gatacaggca ttcattaaac tacgcgcaat
      661 accgcgcccg cgcaattttg gcacgacagc gacattgccg atcatccagc cccgcccgaa
      721 gagactcaag cgccgcacag tcgcgttggc cacaacatcg ccttcttcaa tccagacgaa
      781 gccgggcgcc cagccttcac cttgcggcac aaaactttca gcccaactga acagaaaagc
      841 gagtcgaccg agcaggcgca tctcgcgcac tgccgcgcga ccgctgtcat ccatatcatc
      901 ggcgaaggcg cgatcgatga gattagcgat gctaaccaga tcacgtgaga gattgatcgg
      961 tctagcacca gagaaagtgg cagtgggctg tgtcgcatgc gcgaccaaag tattatcctt
     1021 ccagcgtttt caattaccgc aaatgaaact gcggcttgaa tgactgcaac gcgatgcggt
     1081 gaatcaatca ccaaagttag atgagcccgc tgtcaatccc gcgaaagcgg gaatccagaa
     1141 taataacact ggacacccgc ctgcgtgggt ctgacttagc gtaatatcgg tcagtcgctt
     1201 agccttctaa tgccgctaat aatttggcgg cttcatccga cgtgatcttt ttctcttcga
     1261 ccattttcag aatcgccatg cgctcttgat ccgttacagg ctcaacggcc ggggcgggtg
     1321 tcgatccggc gcgtggtgag tggaagaacg atccccatga gccggcggcg cgttcggtgc
     1381 gcttcttggc gcgatcggtt tggcggtggt gcagccgatc ggcttgacta ccgaagcgct
     1441 cttcgagttg gcgctgcacg cgcgtcattt cagtttcaaa gcgatccatc gccgattcga
     1501 tcatcgattc gacgtgatcg gaaatgccat cgacgcccca ctggctgtcg ccggacaaga
     1561 tcaacacatc gccgcgcgcc gataaattga caatggcgga tccgtcgccg agcacgccgg
     1621 tggcggctcg atcgttgcct tgccattcag tcatcggcac gcggctgcga agttcgccct
     1681 tgctcgccag attcattttt gcgccgccgc ctaaaaccac cagcacgata tcgccgttgg
     1741 cattggcgtt gtagttttga ttgggccgga gttcggtctc gatttttaga tcgccatcga
     1801 tcttgccgaa ggatgcaccc gcgcccagat cttcggcgac gagatcgccg gacacccgat
     1861 tgatgctgag cggcccggcg acgtgcttca ctttgacatc gccgcgcacg gtttcgatgc
     1921 gcgcttcgcc ctcaacatcg cgcagctcga gatcgccttg caccgttttg atcatcagcg
     1981 ggccaatctc gcgcagcgtg gcgtcgccgt tgacggtgtc gatggctaac gcgccggtca
     2041 attgaatgac gcgcaaatcg ccgctgacca actgaatcgt caccgtcgaa ttcagcgggc
     2101 acatgatctc gcaatcatcc agcgcattaa tggtgatcgt ctcgccgctg cgatcgatct
     2161 ttgacgcggg tgaatcgtcg tcgatctcga tcaagacgcg ctgatcgctt gaaccaatga
     2221 gcgataggtc atcgtggcag ctgatggtga tgtgcggggt aggggaggtc tcgtattgcg
     2281 tagtcgtcat gatgtttgct ccattattgt actggtattt tgatcattcg acaaaaacgt
     2341 ttttagtgtg ggcagtggtt caattcgtgc cccaagctgt ggatcagtcc acaaaatttc
     2401 tacgcgctga ttgtcggtca tgtcttccac agcctttgtt ttcattgttc attgttaatt
     2461 gatcattgat aattgtctca gtattgcccc agcgtgccac gtcgacgcac gcggacctcg
     2521 agtgcgcgaa aagcgatcag tcctaaacca atcatcacga acatgaacac gatcagtagg
     2581 atgacctcta cgcccagcgg cagaatcgtc tgcgtgccaa gcagccagcc gcgcaccgca
     2641 tcgaagccgt aggtcaaagg cagcgccagc gcgatcggta gtaaccagcg tggcatggag
     2701 ttcaccggaa aatctgcgcc ggaaaaaatc tgcaccagga agctgctcac gtcaaccagt
     2761 gtatttgctt cgcgtaggat caagacgagt gcggcgaaag caaacccgaa gccgtacaag
     2821 cctagcagca tcggcagcat agtcagaaaa gccgccagcg cattgccgct tggccggaag
     2881 ccgaagatca ctccagccag caataacatg atcaacgaag ttgctgtggt aatgatcaaa
     2941 tttgtgatcg tgtgaccgac cagcagcaag acgcgcggca ccggcgccag ccaactcgat
     3001 tcaagcacgc ccacatccat atcctgtttc aaggagtagc ccatgcccca gaatactgag
     3061 ttaataaaat tactcaaagc ggtgccaagc aaaatgaa
//
LOCUS       DASRTJ010000063         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_306418,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000063 DASRTJ010000000
VERSION     DASRTJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306418"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFF70_01145"
     CDS             complement(<1..454)
                     /locus_tag="VFF70_01145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43329.1"
                     /translation="MSNEHIRTNNPSQLAVIIVSWNVRVLLAGCLDSLYRDLDSSKVD
                     ARVLVIDNASTDGSVEMIRDKFPRAELTASEVNLGFAGGNNQGLALLDFKGLALPQAP
                     YILFLNPDTEVQSGAIKTMLEFINANPKAAVVTSQLSYGDGTFQHSAFH"
     gene            complement(473..>2495)
                     /gene="phnE"
                     /locus_tag="VFF70_01150"
     CDS             complement(473..>2495)
                     /gene="phnE"
                     /locus_tag="VFF70_01150"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZY43330.1"
                     /translation="DVRLDRLITRFSDAKIIFTAISQPDLFTRDTAQQSGQIVFWVPC
                     SNPPQALPTPSAPNFISLDTACGVAGDTVTLHGENFMPGEVGTLHMYAVGGTNESQIF
                     NDNQAIRPVVGADGKFDITFKITKFAEAEGVDPSAPLEKSIEAQFVQQVGPIKPSETF
                     YDIILGKVNEVGVFVPSKMLETLALGILATIFSTLLAVPLSFFAAHNIMSRIPGGSII
                     YYLMRTFLNFVRAVDPIIWGLIIISWLGLGTFTGFMALTIHSTAALAKLFSEEIEHID
                     LGPVEAVSATGANLTQVLRYAVVPQIYPPFLAYTLLRWDINMRSATIIGFVAGGGIGV
                     FVVETIRKGYYAQYAAALWVIAIVIMLVDYMSGVWRQRILLGRTTKTVVVVLGERIDD
                     KLIEGREQNHQATLNPIESAPLPFYRSLRSLVIIAIGIVVFVYCWQISKIDLRKMFDP
                     SPTFGAIVSDFIHIDLSPDVLEVVLRQMLVTIFQALIATTFGAALAIPFSFLAARNLT
                     GRGVIPRVIYYITRSVFNILRSIEALLYAAIFVFWVGIGAFAGALALTVATFALLGKL
                     FSEAIENIDAGPLEAITATGANRLQVIVYGILPQIIPPFVSYMIYQWDINVRISTIIG
                     FAGGQGIGLLLNNYFGQLQYHKAGTVTLFIVLVIIALDFASAKIRERMV"
BASE COUNT          707 a          637 c          606 g          545 t
ORIGIN      
        1 aatggaacgc gctgtgctga aatgttccgt cgccataaga tagttgtgaa gtcacaacag
       61 cggctttggg attggcatta atgaattcaa gcatcgtctt gatcgcgccc gattggactt
      121 cggtgtcggg attcaagaac aaaatatatg gagcctgagg caaagccaaa cctttgaagt
      181 ccagtaacgc caacccctga ttgttgccgc ctgcgaagcc taaatttact tcactggcag
      241 ttaattcagc gcgaggaaac ttatcccgga tcatttcgac gctgccgtcc gtcgaggcgt
      301 tatcgatcac caggacgcgc gcatcaactt tggatgaatc aaggtcgcga tacaacgaat
      361 cgaggcagcc cgctaaaaga acgcggacat tccatgaaac gatgatgacg gcaagttgcg
      421 agggattatt agttctgata tgttcattgc tcattgtaaa ttgacaattg tctcagacca
      481 tccgctcacg gattttggcg ctagcgaaat ccagtgcgat gatgaccagc actatgaaca
      541 atgttaccgt gccagccttg tgatattgca actggccgaa atagttatta agcagcaagc
      601 caatgccttg accgccagca aagccgatga tggttgatat acgcacgttg atgtcccatt
      661 gataaatcat atatgagacg aacggcggga taatctgcgg caagatacca tacacaatca
      721 cttgcaatcg attcgcgccc gtggccgtaa tcgcttccag cggcccggca tcgatattct
      781 caattgcctc tgagaaaagt ttgcccagca aagcgaaggt ggctacagtt aaggccaagg
      841 cgcccgcaaa tgcgccaata ccgacccaga atacgaagat ggctgcatac aacaaggcct
      901 cgatcgagcg caagatattg aagacactgc gagtgatgta gtagatcact cgtggaatca
      961 ctccgcgccc cgtcaagtta cgcgcagcga gaaaactgaa cgggatcgcg agtgccgccc
     1021 caaaggtggt cgcaattaac gcctggaaaa tcgtgaccag catctgcctg agcacgactt
     1081 ctaatacatc gggactcaga tcgatgtgaa taaaatcgct cacgatagca ccaaatgtgg
     1141 gcgaaggatc aaacatcttg cgcagatcaa tcttagagat ttgccaacag tatacaaaga
     1201 cgacgatgcc aatcgcgata atgactagcg agcgcaacga acgataaaac ggtaagggcg
     1261 cagactcaat cgggttaagg gtggcttgat gattttgctc acgcccttca atcaacttat
     1321 catctatgcg ctcgcccaat actacaacca cggtcttcgt agttcggcct agcaaaatcc
     1381 tttgccgcca cacaccgctc atgtaatcga ccagcatgat cacaatcgcg atgacccata
     1441 atgcggcggc gtattgggcg tagtaaccct tgcggatcgt ttcgactaca aagacaccga
     1501 taccgccacc ggcaacaaaa ccgatgatag tcgccgatct catgttaatg tcccagcgca
     1561 acaatgtata ggccaaaaac ggcgggtaga tttgcggtac gacagcataa cgcaggactt
     1621 gcgtcaagtt cgcgccagtt gcagatactg cctctactgg tccaagatca atatgctcaa
     1681 tttcctcaga aaagagttta gccagggcgg ccgtcgagtg aatggttaaa gccataaagc
     1741 ccgtaaatgt ccccagacct aaccacgaaa tgatgatcaa accccaaatg ataggatcga
     1801 ctgctcgcac aaaattcaag aaggtgcgca tcagataata aatgatcgag ccgcccggaa
     1861 tgcgcgacat gatattgtgc gcggcaaaga aacttaaggg cacagccagg agtgtcgaga
     1921 aaatcgtagc caggatgccc agtgccaaag tctccagcat cttggatgga acaaaaacac
     1981 cgacttcatt gaccttgccc agaataatgt cgtaaaatgt ttcgctgggc ttgataggac
     2041 ccacttgctg gacgaattgc gcctcgatcg atttttccaa aggcgcagac ggatcgacgc
     2101 cttcggcttc agcgaatttg gtaatcttga aagtaatatc gaatttgcca tccgccccga
     2161 caaccggccg aatcgcttgg ttatcattga agatttgtga ctcattcgtg ccgccgacag
     2221 cgtacatatg cagcgtgcca acttcacccg gcataaagtt ctcaccgtgc aatgtgactg
     2281 tatcgccagc aacaccacag gccgtatcaa gtgaaataaa gtttggagcg cttggcgttg
     2341 gcagtgcctg cggcggattg ctgcacggca cccaaaatac aatttgcccg ctctgttgtg
     2401 ccgtgtcgcg cgtaaacaaa tcgggttgag agatagccgt aaaaataatc tttgcatctg
     2461 agaaacgcgt aatcaaccga tcgagtctca cgtcg
//
LOCUS       DASRTJ010000064         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_79296,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000064 DASRTJ010000000
VERSION     DASRTJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79296"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFF70_01155"
     CDS             complement(<1..201)
                     /locus_tag="VFF70_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43331.1"
                     /translation="MQKIKELIPKPTNMDTTARSLWVMRRALLLSSFPLGVLTLGIPI
                     YGPSNLHINATQVGTLFSVYALM"
     gene            complement(284..496)
                     /locus_tag="VFF70_01160"
     CDS             complement(284..496)
                     /locus_tag="VFF70_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HZY43332.1"
                     /translation="MPIDIQVGDTVRMRKPHPCGGTDWQITRIGADIGLKCLTCDHRV
                     MLTRRDFEKRLKTILQRADVASTGKL"
     gene            604..894
                     /locus_tag="VFF70_01165"
     CDS             604..894
                     /locus_tag="VFF70_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43333.1"
                     /translation="MRLRRLLLIVAIGLAGCTSVSTAEVPTINAPAVTASPINSAAAD
                     QSTALPKLERTPLPTGPKAPAITNQVWLNTDKPLTQADLSGKVILIDFWTFG"
     gene            904..1230
                     /locus_tag="VFF70_01170"
     CDS             904..1230
                     /locus_tag="VFF70_01170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZY43334.1"
                     /translation="MHTIPAVRELYSLYKDHGLIVIGMHSPEFDYEKQLNNVTDAIAR
                     LDVPYPVALDNDFTIWNAFRNRYWPSLYFIDKRGVIRRNHIGELHQNTSEWTEATQLI
                     ETLLKE"
     gene            complement(1314..1730)
                     /locus_tag="VFF70_01175"
     CDS             complement(1314..1730)
                     /locus_tag="VFF70_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZY43335.1"
                     /translation="MPEYRVHQIEEVLRRLHKTVEGLRGTVVVSIEGFVVAAYSGDGR
                     LRTDNPVDSPQIAAMAASIIALGERVLGRLSRGEIDRILLDGTQGGIVVVPAGREVAL
                     AVMVSKDAKLGLVMYEVRRAAEQVLKVLAGGVEESK"
     gene            complement(1930..3603)
                     /locus_tag="VFF70_01180"
     CDS             complement(1930..3603)
                     /locus_tag="VFF70_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZY43336.1"
                     /translation="MLQKWMRIFTTLSLSGVLVFALILILGASAARSASAHPIGLSSA
                     PAPLQSTAAFTIGLQLVANGLAQPVYVTSAGDDSGRLFVVERTGLIRVIKNGAVLGSN
                     YLDIQAKVESSGDEQGLLSMAFDPNYKTNGTFYVNYTTDASGHDGDTIIARYIVSNPL
                     SDTAVILTVTHLITIFQPETNHNGGQIQFGPNDDYLYIGMGDGGGGGDQHGTIGNGQD
                     LSALLGKILRIDVRGVPTYTIPASNPFTQTVGAKKEIWAYGLRNPWRFSFDRATGDMY
                     IGDVGQNCWEEIDYQPASDPGGLNYGWRLMEGLHTYPDDSGNCSQPTPTLITLTKPIT
                     NYNHTVGSVALGGYVYRGVQYPCIQGIYFFADYGTGEIWSEQQIAPGVWSSAHQLYAG
                     FSISSFGEDQDGELYAVEYGGAIHKLVSSCPANLSSSTKSVSPNLSQSGGGVTYTIVL
                     RNLGSFFNNTLRVTDTLPSGLSYVSGSFTSTRGTPDASSAPTLKWSGSMSNTFVVTLT
                     YRVTVSTGTPQVLTNYASIDPGFAALFTRTAVIIVNGVRVYLPIVLKNY"
     gene            complement(3700..>5363)
                     /locus_tag="VFF70_01185"
     CDS             complement(3700..>5363)
                     /locus_tag="VFF70_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZY43337.1"
                     /translation="LSTIVALTALAFCVLALMLSAAAGRSAVAQPNGISSVPLTTQST
                     PPFEINLELIANGFPQLTQVTHADDDSGRLFVVERAGRIRVIANGLVVTPFLDIQSIV
                     ESGSDWERGLLSVAFDPDYKTNGTFYVDYTSSITGHVGDIVVARYMVSDPLANVADVI
                     VVTNVMTIYHPDFDHNGGQLQFGPQDDYLYISTGDGSSHPSPLDGIGNSQSLTTTLGK
                     ILRIDVHGLPTYTIPASNPFTLTMGTRPEIWAYGLRNPWRFSFDRSNGDLYLGDVGAN
                     CWEEIDYQPASSHGGENYGWRLVEGFHYFKLDDPSNCNQPMVPPQILITLTRPITNYN
                     HSVGTAVIGGGVYRGQQYPWLIGLFLFGDLGTGQIWAGRQSAPGIWSTAFLLDSNLNL
                     TSFGEDQAGEWYVTDLNGAVYKIISKRADLATSSKQVSNRVPRFGDTLTYSIVLRNPA
                     NLITNTIRVTDVIPIGLSYLTGSFTTAHGNVDESAAPILIWTGELSNTPLLTLTYAVT
                     VSTADKEAITNTALIDPSVSPPFVRSVSIFVNGWRSYLPIIFKSF"
BASE COUNT         1330 a         1460 c         1392 g         1181 t
ORIGIN      
        1 catcagcgcg tagacgctaa agagtgtgcc gacctgagtg gcattgatat gcaaattaga
       61 tgggccgtag attggaatgc cgagtgtgag cacaccgagg ggaaaggacg acagcaacag
      121 cgcgcgccgc atcacccata gtgaacgggc ggtggtatcc atgttggtgg gcttgggaat
      181 aagctcttta attttctgca tcaagtattc agtctcggcg ttcggtgatg cgcgttcgtg
      241 attgtgcgcc gaagattata gcacactcga aatgtgccag acttcaaagt ttgccagttg
      301 aagccacatc ggcccgctgc aagatcgttt taagccgctt ctcaaaatcg cggcgagtca
      361 gcataacgcg gtggtcgcag gtcaaacact tcaggccaat atctgccccg atgcgcgtga
      421 tctgccaatc cgtcccgccg cacggatgcg gcttgcgcat gcgaacggta tcgcctactt
      481 gtatatcgat cggcatgtga tctcctgagg cgattatacc catgtaggac tagttggcaa
      541 ctggccctac atgtaagacg cttgtaatga acagtgccta tactatgact aatgagaggt
      601 aacatgcgac tccgcagatt gctattgatt gttgcgattg gtctagcagg ctgcacatct
      661 gtttcgacag cagaagtgcc aaccataaat gcgcccgcag ttacagccag cccgatcaat
      721 tcagcggcgg ctgatcaatc gactgccctg cctaagctgg agaggacacc cttgccgact
      781 ggaccgaaag cccccgccat caccaatcaa gtttggctca acacggacaa accgttgact
      841 caagccgatc ttagcggcaa agtgatcctg atcgatttct ggacctttgg ttgaattaat
      901 tgcgtgcaca caattcccgc ggtgcgggaa ctttactcgc tatacaaaga ccacggcttg
      961 atcgttattg gcatgcattc gcctgaattt gattacgaga agcagctaaa caatgtaaca
     1021 gatgccatcg ctcgattgga cgtcccatat ccggtggcgc tcgacaatga cttcacgata
     1081 tggaacgcat tcaggaatcg ctactggccg tcgttatact tcattgacaa gcgcggcgtc
     1141 attcgacgga atcacatcgg tgaactgcat caaaatacca gcgagtggac tgaagccacg
     1201 cagttgatcg aaacactgtt gaaagagtaa gggcgaggtc acctcgccgc tacgaagaaa
     1261 acagaaacgc ccgatcgaat caatcgattg ggcgtttcac tgtctgcgtg tatttacttg
     1321 ctttcttcta cgccgccagc cagcactttc aaaacttgct cggcggcgcg gcgcacttca
     1381 tacattacca agcctaattt agcgtccttg ctgaccatca cagccagtgc gacttctctg
     1441 ccggcgggca ccacgacgat accgccttgt gtgccatcca acaaaatccg atcgatctcg
     1501 ccgcgcgaca aacggcccag cacccgctcg cccaacgcaa tgatcgatgc ggccattgcc
     1561 gcgatctgcg gactatcgac cggattgtcg gtgcgcaagc gtccgtcgcc ggagtaggct
     1621 gccaccacaa agccttcgat gctgaccacc actgtgccgc gcaagccttc gacggtctta
     1681 tgcaaacggc gcaacacttc ctcaatttga tgcacccgat attcgggcat gtgacctcat
     1741 tgaataaccg cgtgcaggtt gatcggtatg aaagtttgct aactttgaac ctgtgagagg
     1801 tttatcacta aagtataccg caatcgacaa acaatgacca atgaaaaatg atcactggca
     1861 ataaaaacaa gtccggcctc ttcattagac aagaaaccgg acgatagtaa aaggctgcct
     1921 gcaaaaagat tagtaattct tgagcacaat cggcaggtag acgcgcacgc cgttaacaat
     1981 gatcaccgcc gttcgcgtaa acagcgctgc aaagcccgga tcgatcgagg cgtagttcgt
     2041 tagaacttgc ggcgtgcccg ttgagaccgt cacgcgatag gtcaacgtca ccacaaaagt
     2101 gttcgacatc gatccgctcc acttcaaagt cggggctgaa gaagcatcgg gcgtgccgcg
     2161 ggtcgaggtg aatgatccgc tcacatagct caagccgctg ggcagcgtat cggtgacgcg
     2221 cagcgtgttg ttaaagaacg agccgaggtt gcgcagcacg atcgtatatg tgaccccgcc
     2281 gccggattgt gacagattcg gcgagacgga cttcgtgctc gatgaaagat tagccgggca
     2341 tgaagacacg agtttatgga tcgcgccgcc gtattcgact gcatatagtt cgccgtcttg
     2401 atcttcacca aacgaagaaa tgctaaaacc cgcatatagc tggtgagcac tcgaccaaac
     2461 gccgggcgcg atctgctgct cggaccaaat ttcgccagta ccataatcgg caaaaaagta
     2521 gataccttga atgcaaggat actgcacgcc acgatagaca tagccgccca gcgccacgct
     2581 gccaacggtg tggttatagt tggtaatcgg cttggtgagc gtgatcaggg ttggtgtcgg
     2641 ttgactacaa ttgcctgaat cgtccggata agtgtgcagg ccttccatca aacgccaacc
     2701 ataattcaat ccgccgggat cgctggccgg ttgataatcg atctcttccc aacaattttg
     2761 cccgacatca ccaatataca tatcgcccgt cgctcgatcg aacgagaagc gccacggatt
     2821 gcgcaagcca tatgcccaga tctctttctt ggccccgacc gtttgtgtaa acggattgga
     2881 agcgggaatc gtgtacgttg gcacaccgcg cacatcgatc cgcaaaatct tcccgagcag
     2941 cgcgctgaga tcttgtccat taccgatcgt gccgtgttga tcgccgcccc caccgccatc
     3001 gcccatgccg atgtacaaat aatcgtcgtt cggcccaaat tgaatttgac cgccattgtg
     3061 attcgtttca ggctgaaaaa tcgtgatcaa atgggtgacg gtcaagatca ccgccgtgtc
     3121 cgataatgga ttggagacga tatagcgcgc aatgatcgta tcgccgtcat ggccggatgc
     3181 atccgttgtg tagttcacgt agaacgtgcc gttcgtttta taattcggat cgaaggccat
     3241 gctcaacaga ccctgttcat ccccgctgga ttccaccttc gcctgaatgt ctagatagtt
     3301 cgaacccaat accgctccgt ttttgatcac gcggatgagt ccggtacgct cgaccacaaa
     3361 cagtcgcccg ctatcatcac ccgcgctggt cacatacaca ggctgtgcga gtccgttggc
     3421 caccagctgt aagccgatgg tgaaggcggc ggtcgattga agcggcgcgg gagcagagct
     3481 caacccgatc ggatgagctg aggccgatcg agctgcgctt gcgcccaaaa tcaaaatcag
     3541 cgcaaacacc agcacaccgg aaaggcttaa cgttgtgaat atgcgcatcc atttttgaag
     3601 catcgccgca atcctcctga gatgattgag tctatcatcg cccggcaaca ttcggcaatt
     3661 gtactttggt cagaagccgt gtgagttcaa gcgggggtgt cagaaacttt tgaagatgat
     3721 cggcaagtag ctgcgccagc cgttgacgaa gatcgaaacc gatcggacga acggcgggct
     3781 gacgctggga tcgatcagcg cagtgttcgt gatagcttct ttatccgctg ttgaaacggt
     3841 cacagcgtac gtcagcgtta acagcggcgt gttcgacaat tcgcccgtcc agatcaaaat
     3901 aggcgcggcg gattcatcca cgttgccatg cgccgtggtg aatgaacccg ttaagtagct
     3961 caagccgatc gggatcacat ctgtcacgcg gattgtgttc gtaatcaagt tagcgggatt
     4021 gcgcaagacg attgaataag tcaacgtatc gccgaaacgc ggcacgcggt tggaaacctg
     4081 cttgctggat gtggccagat ctgcgcgttt agaaatgatt ttgtagactg caccgttcag
     4141 gtcagtcaca taccattcgc cagcttgatc ttcaccaaaa gaagtaaggt tcaaattcga
     4201 atccaacaaa aacgcagttg accagatccc gggcgcgctc tgccgcccgg cccagatttg
     4261 tcccgtgccc aagtcgccaa agagaaacaa tccaatcagc cacggatatt gctggccgcg
     4321 atagactccg ccgccgatga ccgcagtacc cacgctgtga ttgtaattcg tgatcggccg
     4381 ggtcaatgtg atcaaaatct gtggaggcac catcggctga ttacaattcg atggatcgtc
     4441 caatttgaaa tagtgaaagc cttccaccaa gcgccacccg taattttctc caccgtgact
     4501 gctggccggt tgataatcga tctcttccca acaattcgcg ccgacatcgc caagatacag
     4561 atcgccgttc gatcgatcga acgagaaccg ccatggatta cgcagaccgt acgcccaaat
     4621 ttccggtcgt gtgcccatcg tcagagtgaa cggattcgaa gcgggaatcg tataggtcgg
     4681 caggccgtgc acatcaatgc gcaaaatttt gccgagcgtt gtcgtcaaac tttgcgaatt
     4741 gccgatcccg tcaagtgggc tgggatgact gctgccatcg ccggtgctga tatacagata
     4801 atcatcctgc ggtccgaatt gaagttgacc gccgttgtga tcaaaatccg gatgatagat
     4861 tgtcatgacg ttcgttacca cgatcacatc tgctacattc gccagcggat cggaaaccat
     4921 atagcgtgcg accacgatgt cgcctacatg gccggtgatg ctggaggtgt aatcaacgta
     4981 gaacgtgcca tttgttttgt aatctggatc aaaggccaca ctgagcagac cgcgctccca
     5041 atcgctgcca gactcaacga tcgattgaat gtccaaaaac ggcgtcacaa ccagcccatt
     5101 agcgatcact cgaatccggc ctgctcgctc aacgacgaac aagcggccac tatcatcgtc
     5161 ggcgtgagta acctgcgtca gttgtgggaa gccattggcg ataagttcga ggttgatttc
     5221 aaacggcggt gtcgattgag tggtgagtgg aactgagcta atcccgttcg gctgagcaac
     5281 ggccgaccga ccggctgccg cgcttaacat cagcgccaaa acgcagaaag ccagtgcggt
     5341 cagagcaacg attgtgctta agc
//
LOCUS       DASRTJ010000065         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_41617,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000065 DASRTJ010000000
VERSION     DASRTJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41617"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="rplI"
                     /locus_tag="VFF70_01190"
     CDS             <1..357
                     /gene="rplI"
                     /locus_tag="VFF70_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZY43338.1"
                     /translation="AGAVKQSESLSKAEQKRQSVLATEASALAEQLQAVSLTFHMRAG
                     EEGKLYGSVTASNIVDELKRTTGLELDRHKLELREPLRSLGAHKVAVRLASELAPEMT
                     VHVVREETGSTEPAAA"
     gene            438..1415
                     /locus_tag="VFF70_01195"
     CDS             438..1415
                     /locus_tag="VFF70_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZY43339.1"
                     /translation="MSDLGSQLRTAREAKGLSLEQVFKATRIKLSYLEALESNRNDAL
                     PGLVQARGFVRTYANYLGLDGEILASALDSGNLIMALPTSPAVSTPIEPAKPIGPPIR
                     TTPIDPVATKPLAVTSTSTIAKPDRSSAPTQSTAKLRVPTLSATSMARPGPSTRGIPT
                     PLLIVGAVMLFLIGALLIITALSSGPKSALPPESATLDGTLVINSEVDPTRSIVLNTL
                     PMSLSLTASEHVWVRVTTDGQTAFEGMLVPMATQSWTARDQIIVETGNAAALTVTRDG
                     QTTVLGNRGQVVARAWGHGTVEDMPIAAPDHTSDLPAAPTSIATTTIKP"
     gene            1526..2890
                     /gene="rimO"
                     /locus_tag="VFF70_01200"
     CDS             1526..2890
                     /gene="rimO"
                     /locus_tag="VFF70_01200"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZY43340.1"
                     /translation="MKSKRYYLLTLGCAKNSVDSDSMAQLLNTSGYESVAEPDDASIL
                     IVNTCGFIGPSRAESIRTLRELADGKSKQQTLIAAGCLSQIWGSMLVDEVPGIDGVIG
                     TRRWMDIVEFTDRVRTSKHPQPLYHLPEEAKVVGTDEHGALRASIAGASAYIKIADGC
                     RRPCAFCSIPRIKGTAVSRPAEAIIHEARVLADRGVREIILIAQDLTDYGHDLGMKDG
                     LPNLLESICEAAPGLKWIRLMYAYPGAVSDRLIEVMATRPQIVHYLDIPLQHGHPDVL
                     QRMRRPANIDWVYRTLEKVRTAMPDVAIRTTFIVGYPGETDAEFDGLMKFVRDLKFDR
                     VGAFQYSFEPATPSADQPDQVSEEVKQERYDRLMHAQQSISLKSNQAFVGRTLEVLIE
                     GQGDGLTLGRSYRDAPEIDGMVILEGTAPIGEFVPVKIDGALNYDLSGHVEMIEPQVV
                     VGAR"
     gene            2909..3538
                     /locus_tag="VFF70_01205"
     CDS             2909..3538
                     /locus_tag="VFF70_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZY43341.1"
                     /translation="MISRFLKAIFIIIFIGAVWTFYRAMHIERWIDALLAGKPIGSAP
                     QSPDSAFTPEVLYWWPNIQQWANQFNLDPDLVATVMQVESCGDPNAISSSGAQGLFQV
                     MPFHFTLNAAALDPATNANAGLAFLVEVLNQARGDANLAMAAYNGGAGVIGLQADQWS
                     AETRSYYYWVSGIYSEAKRGSNARLQEWLRAGGSTLCQQADRSLGIKLN"
     gene            complement(3634..3768)
                     /locus_tag="VFF70_01210"
     CDS             complement(3634..3768)
                     /locus_tag="VFF70_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43342.1"
                     /translation="MDVMVGLLLLTVFAGLHHAGSGRNRADLFVVTLLLGAIIIFTHG
                     "
BASE COUNT          890 a         1068 c         1128 g          866 t
ORIGIN      
        1 gccggcgcag tgaaacagtc cgagtcgctg tccaaagccg agcagaagcg ccaatcggtg
       61 ttagcgactg aagcatccgc tttggctgag cagttgcaag cggtgtcgtt gacgttccat
      121 atgcgcgccg gcgaagaagg caagttgtat ggctcggtga cggcgtccaa cattgtcgat
      181 gaactcaagc gcaccaccgg tctcgaactg gatcggcata agctggaact gcgtgaaccg
      241 ctgcgctcac tcggcgcgca caaagttgct gtgcgtctgg ccagcgaact cgcgcccgag
      301 atgaccgttc acgtggtgcg cgaagagacc ggctcaaccg aacctgccgc ggcttaattc
      361 gatccacgca gaacacgcag aaacacgccg aggaattcat tcgtgtcatc cgtgcgatcg
      421 tggttgattg aaaccccatg agtgacctcg gttctcaact acgcacggcg cgagaggcca
      481 aaggcttgag tctggaacaa gtcttcaagg ctacccgcat caaactatcc tatctcgaag
      541 cactcgaatc gaatcgcaac gacgcgctgc ctggcctggt ccaggcgcgc ggttttgtac
      601 ggacctacgc caactattta gggctcgatg gtgaaatact ggcctcggcg ctcgatagcg
      661 gcaacttgat catggcactg ccgacttcac cagctgtgtc aacaccgatc gaaccagcta
      721 agccaatcgg acccccgatc cgcaccacac cgatcgatcc ggtggccaca aaaccgctcg
      781 ctgtaacctc gacctcgacc atcgctaaac ccgatcgatc ttcggcgccg acacaatcaa
      841 cagccaaact gcgtgtaccg acgttgtcag ccacttcgat ggcgcgacca ggtccatcga
      901 cgcgcggcat tcctacaccg ctgttgatcg tgggagcagt gatgttgttt ctgatcggtg
      961 cgttgttgat catcacggct ttgtcaagcg gaccaaagtc tgcgctgccg cctgagtcag
     1021 cgacgcttga tggcacgtta gtcattaact cggaggtaga tcccacgcga tcgatcgtgt
     1081 tgaataccct accgatgtct ctctcgctaa cggccagcga gcatgtgtgg gtgcgtgtca
     1141 cgactgatgg tcaaactgct ttcgaaggga tgcttgtgcc gatggccact caatcttgga
     1201 ccgcgcgcga tcagatcatt gtcgaaacag gcaatgccgc cgcactgacg gtgacgcgcg
     1261 atggtcaaac tacggtgcta ggaaatcgcg ggcaagtggt ggcgcgcgcg tgggggcatg
     1321 gcactgtcga agatatgccg atcgccgcgc ccgatcatac gagcgatcta ccggctgcac
     1381 ccacctcgat cgcgacgacg acgattaaac cctgatacac ggcagtagat tatttaccgc
     1441 agagatacgg atgcgcggcg atattattaa ttctgcgtct cagcatccat gcggtttgat
     1501 ttacttatcg ggtacaataa ctctcatgaa atctaaacgc tactacttgt taactttagg
     1561 ttgcgccaaa aactcagttg attctgacag catggcgcag ctgctgaata cttccgggta
     1621 cgaatccgtc gccgaaccgg atgatgcctc gatcttgatc gtcaacacct gcggcttcat
     1681 cggcccgtcg cgcgctgaat caatccgcac gctgcgagag ttggctgacg gcaaatccaa
     1741 gcagcaaact ctgattgcgg cgggctgcct ctcgcaaatc tggggatcga tgttagtgga
     1801 cgaagtgcct ggcattgatg gcgtgatcgg cacacggcgc tggatggaca tcgtcgagtt
     1861 caccgatcga gtgcgcacga gcaaacaccc gcagccgttg tatcatttac ctgaagaagc
     1921 gaaagttgtc ggcacggatg aacatggtgc gctgcgtgca tcgatcgccg gcgcgagcgc
     1981 ttacatcaag attgctgatg gctgccgccg accgtgcgcg ttttgttcga tcccgcgcat
     2041 caaaggcacg gcggtcagcc gcccggccga ggcgatcatt cacgaagcac gggtgctggc
     2101 cgatcgaggt gtgcgcgaga tcattttgat cgcgcaagac ctgaccgatt acgggcacga
     2161 tctgggcatg aaagacggtc tgccgaattt gcttgaatcg atctgcgagg ctgcgccggg
     2221 gttgaaatgg attcgcttga tgtatgctta tccgggcgcg gtgagtgatc gcttgatcga
     2281 agtaatggcg actcgaccgc agatcgttca ttatcttgac atcccattgc aacatgggca
     2341 tccagatgtg ttgcagcgca tgcggcgtcc ggcgaatatc gattgggtgt atcgcacgtt
     2401 ggaaaaagtt cgcacggcca tgcccgatgt cgccattcgc acgaccttca tcgtcggtta
     2461 tccgggcgaa acggatgcag aatttgatgg tttgatgaag tttgtgcggg acttgaaatt
     2521 cgatcgagtg ggtgcgttcc aatattcatt cgagccggcc acaccctcgg ccgatcagcc
     2581 cgatcaagtt tcagaggaag tcaaacaaga acgctacgat cgcttaatgc acgcgcagca
     2641 atcgatctcg ttgaagagca atcaagcgtt cgtcggccgc acgctcgagg tgttgatcga
     2701 agggcagggc gatggcttga cgctgggccg atcgtaccgc gatgctccag agatcgatgg
     2761 catggtgatt ctcgaaggca ccgcccccat cggcgagttt gtgccggtca agatcgacgg
     2821 tgcgctgaac tatgatctca gcggccacgt ggaaatgatc gagccgcagg tagtagttgg
     2881 ggcgaggtaa cctcgcccgt ataacgcaat gatctcccgc ttcctcaaag cgatcttcat
     2941 catcatcttc atcggcgcag tgtggacctt ttatcgcgcg atgcacatcg agcggtggat
     3001 cgatgcgctg ctcgctggta aaccgatcgg atcagctccc caatcgcctg attctgcctt
     3061 tactccagaa gttctatatt ggtggcctaa cattcaacag tgggccaatc aatttaatct
     3121 cgatcctgat ctggtggcga ccgtcatgca ggtcgagtcg tgcggcgatc ccaatgccat
     3181 atcgtccagc ggcgcacagg gtttgtttca agtcatgccg tttcatttca cgctgaatgc
     3241 ggccgcgctc gatccggcga cgaatgcgaa cgccggtctc gcttttttgg tcgaggtgtt
     3301 gaatcaagcg cgaggcgatg ccaatctggc aatggccgcg tacaacggcg gggcgggcgt
     3361 gatcggtttg caagccgatc agtggtcagc tgaaacacgg tcatattact attgggtcag
     3421 cgggatatat tcggaagcga aacgtggatc gaatgcgcgc ttgcaggaat ggctcagagc
     3481 gggaggatca acattgtgtc agcaggcgga tcgaagtttg gggattaaac taaactgatc
     3541 aatctttgat ctccgatatc taggctttga tttactgcaa cccgttcagc gaagtggaaa
     3601 atcagaaatc aaaattcaga gatcagagat tcactagccg tgcgtgaaga tgataatcgc
     3661 gccgagcagc agcgtgacga cgaagaggtc ggcgcggttg cggccactac cggcatggtg
     3721 cagtccggca aagactgtta gcagtagcaa gcccaccatc acatccatgt cacacctccg
     3781 cgcacgcact tgaatattgc tttcatctat tcgacgttga gtacggtaaa aaagtgccag
     3841 ccagccagct caaattgaaa gattgttcga ttgatctgaa gggaagcgat ctggaacttt
     3901 agctcagtga ggcgcgtctg ttatgagtag ggttttcgca tgtcaggctg ag
//
LOCUS       DASRTJ010000066         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_306521,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000066 DASRTJ010000000
VERSION     DASRTJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306521"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFF70_01215"
     CDS             complement(<1..772)
                     /locus_tag="VFF70_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY43343.1"
                     /translation="MKKSLLFTLLILAMITSACAPTITPASIPTSAPVVSVLPTPPSS
                     PLAQPESVLPTPSAARTLTVLTHEAFALSDDVLNKFEADNNVTVNFIKGSDAGPALNR
                     AILTKDNPEADIIFGIDNTLIGRALEAGILDPYKPAALSTISTEFQLDPSGQLTPIDY
                     GDVCLNYAKNYFAQKKLSPPQTLIDLTKPEYKNLLVVENPAGSSTGLAFMLATVSVFG
                     EEGWLKFWQQLKANGVKIDEDWNTAYYTDFSGSSGKGPR"
     gene            complement(801..1442)
                     /locus_tag="VFF70_01220"
     CDS             complement(801..1442)
                     /locus_tag="VFF70_01220"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HZY43344.1"
                     /translation="MRAIIFANGNPPDIQTFQKWSTASALIIAADGGTHNALSIEVRP
                     QVIIGDMDSFDIKDRGWLEKTGAKFIVYPEHKDLTDLELAIRYAVTHGADEIVILSAL
                     GDRWDQTLANMMLLGLPELRQVPTRIVDHTLSIVAVWDRIEIEGRSGDGLSLIPIGGA
                     ALGVSIEGCEYPLSDAVLPFAATLGISNQLIESIARVSVKEGIVLAIHTTRDE"
     regulatory      complement(1523..1632)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          372 a          459 c          429 g          421 t
ORIGIN      
        1 gtctcggccc tttgccgctc gatccactga agtcggtgta atacgccgtg ttccaatctt
       61 catcgatctt cacgccgttc gctttcagtt gttgccaaaa tttcaaccaa ccttcttccc
      121 cgaaaacgga aacagtggct aacatgaacg ctagacccgt tgacgatccg gctggattct
      181 cgacgacgag tagattcttg tattcgggct tggtgagatc gatcaacgtt tgcggcggcg
      241 ataatttctt ctgcgcgaaa taattcttgg cgtagttcaa gcaaacatcg ccataatcga
      301 tcggtgtgag ctgccccgat ggatcgagtt gaaactcagt tgagatagtc gacagcgccg
      361 caggtttata cggatcgagg atccccgcct caagcgcgcg gccgatcaag gtgttatcaa
      421 taccaaagat aatatccgct tcggggttgt ctttggtcaa gatcgctcga ttgagcgccg
      481 ggccagcatc agaccctttg ataaagttga cggtaacatt attatccgct tcgaatttat
      541 ttagcacatc atcgcttagc gcgaacgctt cgtgtgtcaa caccgttaaa gtgcgtgcag
      601 cacttggtgt cggtaatact gactctggct gtgccagagg cgatgagggc ggcgttggca
      661 acactgacac cacaggtgcg ctggtgggta ttgatgctgg cgttatagtc ggcgcgcacg
      721 ccgaggtgat cattgccaag attaacagcg tgaatagcaa actcttcttc atagataaat
      781 ctcctatgac gcgatgcaga ttattcatcg cgggttgtgt gaatcgccag aacaatgcct
      841 tctttgacac tgactcgagc gatcgattcg attaattgat tgctgatgcc taacgtcgcc
      901 gcgaacggca gcactgcatc acttaacgga tactcgcagc cttcgatcga gacgccgagc
      961 gcagcgccgc cgatcgggat cagcgataac ccatcgccgc tgcgcccttc gatctcaatt
     1021 cgatcccaca ccgcgacaat cgacaaagtg tgatcgacga tccgggttgg cacttggcgc
     1081 agctcaggca agcctagcag catcatgttc gccaacgttt gatcccaacg gtcaccgagg
     1141 gcgctcaaga tcacgatctc gtccgcacca tgcgtgaccg cgtagcgaat cgccaattcc
     1201 agatcagtta aatctttgtg ctctggataa acaatgaact ttgcgccggt cttttccagc
     1261 cagccgcggt ccttgatatc gaatgaatcc atgtcgccga tgatgacttg tggccgcacc
     1321 tcgatcgaga gtgcgttgtg cgtgccgcca tcggccgcga tgatcaatgc cgatgcagtt
     1381 gaccactttt gaaatgtttg gatgtccggc ggattgccgt ttgcaaaaat aatcgctcgc
     1441 atgtttgtcc ttcagacccg cgaggtttcc aataacctcg cggttctata atcaaaacaa
     1501 aagccacccg gcttgggtgg ctcaatcttg ctgaacgttc cctccgccgg cattatccgg
     1561 atcaggttcc tgggtcagcg atggagtgat cgccctctca gcccgcctcc gcgagctccc
     1621 catatgtgct tgtggttttg attataccac ttccgttctg cccttgtcga agggatgtga
     1681 g
//
LOCUS       DASRTJ010000067         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_41737,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000067 DASRTJ010000000
VERSION     DASRTJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41737"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFF70_01225"
     CDS             complement(<1..799)
                     /locus_tag="VFF70_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43345.1"
                     /translation="MVYSSHIPSVAEVDRIATLGDPIIRNLQITQSYYVLSAALAACT
                     GLNANWCTFATWASKQAGQTIRQEDLARTLEYVLNTSSATTQTARSVAASAQRIGAKR
                     DLNEIQESVWDVLNSAGAIDRASKAVARGNQKVFEEIGREFARFIDICLRDATFDADH
                     VDHFCEGLRPGGPPDGHRYLRQAFTHYYQSFFESDAKARAELLLLANIEIGFHEQTRL
                     QPEIAAALDAAVVDPKEFKRQLIQAIFPYRGWLARLRLFLTRLVTGPT"
     gene            complement(866..>2209)
                     /locus_tag="VFF70_01230"
     CDS             complement(866..>2209)
                     /locus_tag="VFF70_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43346.1"
                     /translation="RQQSLIGAIDWSYHLLDEEEQRLFDVLGVFVDGYDEEAISDLRS
                     LVDDSIDADSRRQIKDQKSRIRDLVEKSLLRYELAPDGTPRYSMYEMIRDYAHEKLRE
                     RGRLDRARQIHAEHYYRLAQAARQHLTQGSEQVKWLNRLEIEHNNLRAALTWSIESID
                     RAEFAYRLAEALHDFWQIRGHLSEGRRWQEHVLALDAVPSAVRAQLLSNVGQIARMQG
                     DYAHAQTVLEQALAIQQQLNDEAGQCRSLEQLAILAGSHGDYTRARDLLEDMLEKRRL
                     LGDERAVVITMNNLAIATRRLKDFMRAGQLYAECAALSRTLNDQRSLARALYGLGEVR
                     VELDDYAEGLRYFRESLKIRQQLGSRPEIIIALGGAGMAYFWLGDAATAAIIMAASEK
                     HRQDLGMAITPVNRPEVEGNIAQVREKVGAAFETAWAKGRAMTIDEAVALALNSD"
BASE COUNT          467 a          680 c          589 g          473 t
ORIGIN      
        1 gggtggggcc ggtgaccaat cgggtgagga acagccgcag ccgcgccagc cagccgcgat
       61 acggaaaaat cgcttggatc aactgccgct tgaactcttt gggatcgacg acagctgcgt
      121 ccagcgcggc ggcaatttcg ggctgcaagc gcgtctgctc gtgaaacccg atttcaatat
      181 tggctaacaa caagagttca gctcgcgcct tggcatcact ttcgaagaac gattgatagt
      241 agtgtgtgaa agcttgacgc aaataacggt gcccatcggg tggaccgccc ggacgcaacc
      301 cttcgcagaa gtgatcgacg tgatcggcat cgaatgtcgc gtcgcgcagg cagatgtcta
      361 tgaagcgcgc aaactcgcgt ccgatttcct cgaaaacctt ctggttgcca cgagctaccg
      421 ccttgcttgc ccgatcgatc gcaccggcag aattcaaaac atcccacacc gattcctgaa
      481 tctcgttaag gtcgcgcttc gcgccgatgc gctgtgcgga agcggcgacg ctcctggcgg
      541 tctgcgtcgt ggcagacgac gtgttgagca catattccag agtgcgcgcg aggtcttctt
      601 ggcggatggt ctgcccggcc tgcttggagg cccacgtagc gaaagtacac caattcgcgt
      661 tgaggccagt gcacgccgcc agcgccgccg acagtacgta gtagctctgc gtgatctgca
      721 agttgcggat gatcggatcg ccgagcgttg cgatccgatc gacttcggca acgctgggaa
      781 tgtgagaaga atagaccata tcgttcattg ccaagaatcg gaagaagcaa gcggcatggt
      841 cttgaaggcc gaattcaatc cgcggtcaat cgctgttcaa cgccagcgcc actgcttcat
      901 cgatcgtcat cgctcgacct ttggcccacg ccgtttcgaa cgcagcgcca accttctcgc
      961 gcacctgagc aatgttcccc tcaacctcgg ggcgattcac cggcgtgatg gccatgccca
     1021 aatcctgccg gtgtttctcg ctcgcagcca tgatgatcgc cgcggtcgcc gcatcgccca
     1081 gccagaagta ggccatgccg gcaccaccca gcgcaatgat gatctcgggg cggctgccca
     1141 gctgttgtcg aatcttcaaa ctctcgcgga agtaacgcag tccttcagca tagtcatcca
     1201 gttcgacgcg cacttcacct aaaccataca acgcccgggc caatgaacgt tgatcgttca
     1261 gcgtgcggct taatgcggca cactccgcat agagttgccc ggcgcgcatg aaatctttta
     1321 agcgtcgcgt ggcgatggcc agattgttca tcgtgatcac gaccgcacgc tcatcgccca
     1381 ataggcgacg cttctccaac atgtcttcca gtagatcacg cgcgcgtgtg tagtcgccgt
     1441 gactgcccgc cagaatagcg agctgctcca acgatcgaca ttgacccgcc tcatcgttca
     1501 attgctgctg aatcgccagc gcttgttcca gcaccgtctg cgcgtgcgca taatcaccct
     1561 gcatgcgcgc gatttgaccg acgttgctca gcagttgcgc acggacggcg gaaggcacag
     1621 catccagcgc caagacgtgt tcttgccagc gccgtccctc gctgagatgc ccacgaatct
     1681 gccagaaatc gtgcagggct tccgccaagc gataggcgaa ctctgcccga tcgattgatt
     1741 cgatcgacca agtcaacgcc gcacgcaaat tgttgtgctc tatctccaac cgattgagcc
     1801 acttcacctg ctcgctgccc tgggtcaaat gctgacgcgc ggcctgcgcc agccgatagt
     1861 aatgttctgc gtgaatctgc cgtgcccgat cgagccgacc gcgctctcga agtttttcgt
     1921 gcgcataatc gcggatcatc tcgtacatcg aatagcgcgg tgtgccatcc ggcgcaagtt
     1981 catagcgcaa gaggctcttt tcaactaaat ctctgatcct tgatttctga tctttgattt
     2041 gacgcctgct atctgcatct attgaatcat ccaccaaaga tcgaaggtca gagattgctt
     2101 cttcgtcgta gccgtccaca aacacgccca gtacatcaaa caaacgttgt tcttcttcgt
     2161 ccagcaagtg gtaactccaa tcgatcgcgc cgatcaacga ttgctggcg
//
LOCUS       DASRTJ010000068         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_41760,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000068 DASRTJ010000000
VERSION     DASRTJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41760"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFF70_01235"
     CDS             <1..194
                     /locus_tag="VFF70_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZY43347.1"
                     /translation="EGHEPPYMVEGNAVPLEVGNTYTIEPGIYVPGLGGVRIEDDMLI
                     TLNGSESLTTFSRELMLIK"
     gene            complement(284..511)
                     /locus_tag="VFF70_01240"
     CDS             complement(284..511)
                     /locus_tag="VFF70_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY43348.1"
                     /translation="MSMPLYEYQCAECATLFEMLRPIVKADAPIACSNCGSLESSRKL
                     SVFAMVGGSHSIESDSTGGCACGGHCSCGQH"
     gene            complement(644..1828)
                     /locus_tag="VFF70_01245"
     CDS             complement(644..1828)
                     /locus_tag="VFF70_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY43349.1"
                     /translation="MDLFEKARQFTRPQEVKALGLYPYFLPLEDSEGTEVTIYGKKII
                     MIGSNNYLGLTTHPKVREAAIEAIKKYGTSCTGSRFLNGTLKLHMEAEEKLAAFVGKP
                     AALIFSTGMQVNLGAISCLLGKDDIAIIDKDDHASIVDGVRLGFGEMRRFVHSDLASL
                     ERQLAAAPDKAGVLVVVDGVYSMGGDIAPLPAIVKLVKQYGARLYVDDAHSLGVLGPQ
                     GRGTAAHFNLTDQVDLIMGTFSKSFASLGGFVAGDADVMQWVQHSARSFIYSASIAPA
                     SLAAAIAALEIMQTESDRIEQLWHNTEKMRKGYQAMGFNTRPSETPVIPIMIGDDQKT
                     FAAWKALFEAGIYTNAVVSPAVPSSKSLLRTSYMATHTDAQLDRVLDTFQRVGRELGL
                     IN"
     gene            1978..2418
                     /locus_tag="VFF70_01250"
     CDS             1978..2418
                     /locus_tag="VFF70_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZY43350.1"
                     /translation="MGLIRLAKQARSGLTRIARSRSNRRHARRAKALLALHHGQSVQQ
                     VAQATGLSRQAIYNLQKRFLTRQADRLTERLSDGQHTGRPPQKIRAAKKIIQWLLRRS
                     PRKYGYAAEDWTVPMLHQQIQKRTGADVSRRTIRRALNELQSHS"
BASE COUNT          609 a          709 c          640 g          528 t
ORIGIN      
        1 tggaaggaca tgagccgcca tatatggtcg aaggcaatgc ggtgccgttg gaagtgggta
       61 acacgtatac gattgagccg ggcatttatg tgccgggcct aggcggcgtg cggatcgagg
      121 atgatatgtt gatcacgctg aatggttcgg agagcttgac cactttctcg cgtgaattga
      181 tgcttattaa atgaagggat cgggctagta atcactcaat aaaaaaaaac tccggcttca
      241 ccgcgaagcc ggagttatat ttttgatctc actgatcaac tggctaatgc tgtccgcacg
      301 aacaatgacc accgcacgca caaccgccgg tcgaatctga ctcgatcgag tgcgatccgc
      361 cgaccatggc aaagaccgat aactttctcg atgattcgag actgccacag ttcgaacagg
      421 cgatcggcgc atcggctttc acgatcggcc tcagcatctc aaagagtgtt gcgcactcgg
      481 cgcattgata ttcatagagt ggcatactca ttaacctcaa tcctcgtcat tgcgagcgaa
      541 gcgaagcaat ctcagcacat ttaaaagagg gattgcttcg tcgcatccct tcgacttcgc
      601 gcttcgcgct ctgctcagca tgctcctcgc aatgacgacc gtattaattg atcaacccga
      661 gttctctgcc cactcgctgg aacgtatcta aaacccgatc gagctgcgca tccgtatgcg
      721 tcgccatgta ggatgtgcgc agcaggctct tgcttgacgg cacagccggt gaaaccaccg
      781 cattggtata gatgcccgcc tcaaacaacg ctttccacgc cgcaaaagtt ttttgatcgt
      841 cgccgatcat gatcgggatg accggcgtct cgcttggcct ggtattgaag cccatcgcct
      901 gataaccttt gcgcatcttt tccgtattgt gccacaactg ctcgatgcga tcggattcag
      961 tctgcatgat ctcgagcgcc gcgatggcag ccgccaaaga tgcgggcgca atcgaggcgg
     1021 agtagatgaa cgatcgggcg ctatgttgca cccactgcat cacatcggca tcgccagcca
     1081 caaaaccgcc caacgatgcg aacgatttgc taaacgtgcc catgatcaga tcgacttgat
     1141 cggtcaaatt gaaatgcgcg gcggtgccac ggccctgcgg tcccaacaca cccagcgagt
     1201 gcgcatcatc gacgtacaag cgtgcgccat attgtttgac caacttaacg atcgcaggta
     1261 gcggcgcgat gtcgccaccc atgctgtaca caccatcgac cacgaccaac acacccgcct
     1321 tatcgggcgc cgcagctaac tgccgttcta atgaagcgag atcgctgtgc acaaaacgcc
     1381 gcatctcacc aaaacccaag cgcaccccgt cgacgatgct ggcgtgatcg tccttgtcga
     1441 tgatggcaat atcgtccttg ccgagcaaac atgagatggc accgaggttg acctgcatgc
     1501 cggtcgaaaa aataagcgcg gccggcttgc cgacaaaggc cgctaatttt tcttcagcct
     1561 ccatgtgcag cttcaacgtc ccattcaaaa agcgtgagcc ggtacaggac gtgccatatt
     1621 tcttgatcgc ttcgatcgcg gcttcacgaa ctttgggatg ggtcgtcagc cccaaatagt
     1681 tattggaacc gatcatgata atcttcttgc cgtagatggt gacttcggtt ccctcagaat
     1741 cttccagcgg caggaaatat ggatacaagc ccagggcttt gacttcttgc gggcgagtaa
     1801 attggcgtgc cttctcaaat aaatccatgc tgcacctcct gaaacaacga actaagattt
     1861 ctcgatggcc gattgtagcc ctgcttcgaa aaatggtcaa gcgctgctgg acagaatatt
     1921 cttgacaaat tattcaggac ggcttatgct atacacgaag gtcaggaggt tgcaggcatg
     1981 ggcttaatcc gattagctaa acaagcccga tcagggttga cacggatcgc gcgatctcga
     2041 tcgaaccgac ggcacgcccg gcgcgccaaa gcgctgctgg cgctgcatca cggtcaaagc
     2101 gtgcagcaag tggcgcaggc cacgggcctg agccggcaag cgatttataa tctccagaag
     2161 cgcttcttga cgcgccaagc cgatcgatta acggaacgcc tcagcgacgg gcagcacact
     2221 ggccgcccac cccaaaaaat tcgcgcggcc aaaaagatca ttcaatggct gctgcgccgc
     2281 agtccacgca agtatgggta tgccgctgaa gattggaccg tgcccatgct gcatcagcaa
     2341 attcaaaagc gcaccggcgc agatgtcagc cgccgcacta ttcgacgcgc gttaaatgag
     2401 ttacaatcgc actcatgagg tggcagccat gtccgagcaa atgattcaac atgcccaagc
     2461 cgtgaagcgc cagcacgaag ctgact
//
LOCUS       DASRTJ010000069         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_306610,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000069 DASRTJ010000000
VERSION     DASRTJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306610"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VFF70_01255"
     CDS             <1..1374
                     /locus_tag="VFF70_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZY43351.1"
                     /translation="VYVGAVAFIVGIDLTEIEAALSFHFGGKKKPLELNFGVVKAAHE
                     WSQKNLTKTDPYRVEPMNETADKFLIDGNTAAALGAIFGGVQFAAWYPITPATSLADG
                     LNEYLPKLRRDVDGKATYSIVQAEDELAAIGMVVGAGWAGARAMTSTSGPGLSLMAEF
                     AGYAYFAEIPAVIWDVQRMGPSTGLPTRVAQGDVLAAYTLGHGDGKHICLLPASVAEC
                     FEFGSTAFDLAERLQTLVIVLSDLDLGMNLWPTKPFEYPAKPIDRGKVLSAEDLKKLG
                     GKWQRYADVDDDGIGYRTLPGTNHPMAAYFTRGTGHNENAIYSERPEDWSANLVRLAK
                     KHDHARTIVPAPAIYMVKDAEVGIISYGTNDPAILEARDRLESTKIKTSYLRLRALPL
                     ENTLIEFIKKHKRVYVVENNLDGQMYSLVSLHTPENVNRLVSICKCDGLPLTARWITE
                     AILEQER"
     gene            1422..>2344
                     /locus_tag="VFF70_01260"
     CDS             1422..>2344
                     /locus_tag="VFF70_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZY43352.1"
                     /translation="MTTTALRKSASVNLVGLTKTDYKGAPSTLCNGCGHDSISSQIIG
                     VAYDLSIDPYRIAKFSGIGCSSKSPAYFLGRSHSINGLHGRMPSLATGATTVNQELIG
                     IGVSGDGDTGSIGFGQFKHMLRRNVDMVYIIENNGVYGLTKGQFSATADMGQNLKYAG
                     LNELPPIDLCMEAIIADCGFVARSFAGDPKQVQALLKAALSHHGTAVIDIISPCVTFN
                     NGEASTKSYSWGKDHDEPLHEITYIPMEHAEIQVDYEPGTVKDVEMHDGSIIRLTKIG
                     HDYDPTDRMAAIKLLEEAKAKQEFVTGLLYV"
BASE COUNT          542 a          654 c          663 g          485 t
ORIGIN      
        1 gtgtacgtcg gcgcggtagc gttcatcgtc ggcatcgatc tgacagagat cgaggcggcg
       61 ctgtcctttc acttcggcgg gaaaaagaaa ccgctcgagc tcaatttcgg cgtggtcaaa
      121 gccgcgcatg agtggtcgca aaagaatctg accaagactg atccctatcg tgtcgaaccg
      181 atgaacgaga cggcggacaa gttcttgatc gacggcaata cagcggcggc gctcggcgcg
      241 atcttcggcg gcgtgcaatt tgccgcctgg tatccgatca ctccggcgac atctttagcg
      301 gatggcttga atgaatatct gccgaaactg cgccgcgatg tcgatgggaa agctacttac
      361 tcgatcgtgc aggccgaaga tgaactcgcg gcgatcggca tggtcgtcgg tgcgggttgg
      421 gccggtgcgc gcgcgatgac ctcgacctcg gggcccggct tatccttgat ggcggaattt
      481 gcaggctacg cttacttcgc cgaaatcccg gcggtgattt gggatgtgca gcgcatgggt
      541 ccatcgaccg gcttgcccac gcgtgttgcg cagggcgatg tgctggccgc ttacacgctc
      601 ggtcatggcg atggcaaaca tatttgtttg ctgcctgcat cggtggccga atgttttgag
      661 ttcggcagca ccgcgttcga tctggccgaa cgcttgcaga ccttagtgat tgtgttgagc
      721 gatctcgatc tcggcatgaa cctatggccg accaaaccct ttgagtatcc cgctaaaccg
      781 atcgatcgcg gcaaggtcct atcggctgaa gatctcaaga agctgggcgg caaatggcag
      841 cgctatgccg atgtcgatga tgatggcatc ggttatcgca cgctgcccgg caccaatcat
      901 ccaatggcag cctacttcac gcgcggtacc ggccacaatg aaaacgccat ctacagcgag
      961 cgccccgaag attggtcggc aaatctggtg cgtttggcga agaaacatga tcacgcccgc
     1021 acgattgtac cggcaccagc tatttatatg gtcaaagatg cggaagtcgg catcatttcg
     1081 tacggcacaa acgatcccgc gatcctcgaa gcgcgcgatc gattggaatc gacgaaaatc
     1141 aaaacctctt atctgcgcct gcgcgcgctg ccactggaaa acactttgat cgaattcatc
     1201 aagaaacaca agcgagtgta tgtcgtcgag aacaacctcg atggacagat gtattcgctc
     1261 gtgtcactgc acacacccga aaacgtcaat cgtctcgtat cgatctgcaa gtgcgacggt
     1321 ctgcccttga ccgcgcgctg gatcaccgaa gcaattttgg aacaagaacg ataatatatt
     1381 gtaggggcga gcggctgctc gcccatgagg atcaataaat catgactacc acagccttgc
     1441 gtaaatcagc cagcgtcaac ctcgttggct taactaaaac cgattacaaa ggcgcaccct
     1501 cgacgttgtg caacggctgc ggccacgact cgatctccag ccagatcatc ggcgtggcgt
     1561 acgatctctc gatcgatccg tatcgcatcg ccaaattcag cggcatcggc tgctccagta
     1621 aaagcccggc ctacttcctc ggtcgatcgc acagcatcaa cggtctgcac ggccgcatgc
     1681 cttcactggc cacgggcgcg acgacggtca accaggaatt gatcggcatc ggcgtcagcg
     1741 gcgatgggga taccggctcg atcgggtttg gccaattcaa acacatgctg cgccgcaacg
     1801 tcgatatggt ctacatcatt gagaataacg gcgtgtatgg tttgaccaaa ggccaatttt
     1861 ccgcgacggc ggacatgggt cagaacttga agtatgccgg cttgaacgaa ctcccgccga
     1921 tcgatctgtg catggaagcg atcattgccg attgcggttt cgtcgcccga tcgttcgccg
     1981 gcgatcccaa gcaggtgcag gcgctgctca aagcagcctt gagtcatcac ggcacagcgg
     2041 tgatcgacat catcagcccg tgtgtcacct tcaataatgg cgaagcgtcg accaagagtt
     2101 actcgtgggg caaagatcac gacgaaccgc tgcacgagat cacctacatt ccgatggagc
     2161 acgctgagat tcaagtggac tatgaaccgg gcacggtcaa agatgtggag atgcacgacg
     2221 gatcgatcat ccgcttgacg aagatcggcc acgactacga tcccactgat cgcatggcgg
     2281 ccatcaagtt gctggaagag gctaaagcca aacaggaatt tgtaacgggc ctgctgtatg
     2341 tgaa
//
LOCUS       DASRTJ010000070        12719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_115222,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000070 DASRTJ010000000
VERSION     DASRTJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115222"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..227)
                     /locus_tag="VFF70_01265"
     tRNA            complement(156..227)
                     /locus_tag="VFF70_01265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Met,seq:cat)
     gene            complement(284..679)
                     /locus_tag="VFF70_01270"
     CDS             complement(284..679)
                     /locus_tag="VFF70_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43353.1"
                     /translation="MSSSRRPAVWRSPQVWLIIAAIIVLPFLAEFNARLAQSRELDAQ
                     QVALSAQLTTEHARQEALLKLQAWVNSDSYVEHWARLARMVQPGEIAVVPVASQLTSS
                     TSTTQSASGNAAPNDILGEWWTAFFGEPR"
     gene            complement(733..816)
                     /locus_tag="VFF70_01275"
     tRNA            complement(733..816)
                     /locus_tag="VFF70_01275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(780..782),aa:Leu,seq:cag)
     gene            complement(869..2599)
                     /locus_tag="VFF70_01280"
     CDS             complement(869..2599)
                     /locus_tag="VFF70_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY43354.1"
                     /translation="MIRHIRWQVALIVLGALMLGGLLAYLAVAFTTTREPAPGGTYVE
                     GIVGSPQFINPLLCQYNEVDHDLCALIFSGLVHFDEHGLPQPDLADHWDILDNGLTYV
                     FHLRSNLRWQDNQPVTAEDVVFTLQMLKDPNYPGSPDLAALWYTVVISHVDNSTIQMT
                     LQEPLAAFLQYASIGILPKHILGNTAAKDLADSPFNKKPIGTGPFAVETFGSPEAGQS
                     QHILLAANSHYYGKQPFIAKIDFKFFPDFSSMVAAYRAGEIQGLSHVPFANLPAVRDI
                     PTLNLYNAPLDAYTMIYLNQGDDVPFFQDQDVRQALFISIDRQKLIDEILHGQAILAN
                     SPIIPGTWAYHSDLQPPALDLGQARQLLSKAGWQFSSASRPATDDTGLVIVPTPSPVS
                     DEPPIRVKNGVPLSFTLYCNSDPVHIAVAKSIAQQWLAIGVKAAVVPVQAGLASNYLA
                     PRNYQAALIDVQLQGDPDPYSFWDETQANAGQNYSQYRDRDVSEILEQARRTSNISTR
                     IDLYRKFQEMFLERMPGILLYYPIYTYAVSEKVSNVQIGPIAFPADRFRTVADWYVIT
                     RRVIVSSASK"
     gene            complement(2848..3075)
                     /gene="secG"
                     /locus_tag="VFF70_01285"
     CDS             complement(2848..3075)
                     /gene="secG"
                     /locus_tag="VFF70_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZY43355.1"
                     /translation="MQNILNIAQIIVAVGLIIIVILQARSSSAGSMFGGGDSPVYHTR
                     RGIERTLFNATVLLAIVFFILAILNVMSVAV"
     gene            complement(3165..4226)
                     /gene="cax"
                     /locus_tag="VFF70_01290"
     CDS             complement(3165..4226)
                     /gene="cax"
                     /locus_tag="VFF70_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZY43356.1"
                     /translation="MRGLRLLLLFIPIAILAELLHWSPLIVFGSSALAVVPLAALLGE
                     ATEAIAEKIGPRFGGLLNATLGNAAELIITLFAVRAGLLDLVKASIIGSILGNVLLVG
                     GASILAGGLKHGLQRFNRSHAGLDSTLAILAVIALSIPSFFNAAIEPDQVRVEELSLT
                     VAAAMLLIYVLSIVYTIRSRDKHEAATDTSIQPSHTAPRWTTRQSIGILIAATAAIVV
                     MSEFLVSTVEPVSHALGLSEFFIGIIIVPIIGNVAEHVVAVQVALKNQMDLSLSIVMG
                     SSLQIALFVAPVLVFVSLLMGHPLTLEFNSFELVSLIAASLISALVSLDGESNWLEGA
                     TLLIVYAIVALAFFFLPSV"
     gene            complement(4342..5292)
                     /locus_tag="VFF70_01295"
     CDS             complement(4342..5292)
                     /locus_tag="VFF70_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZY43357.1"
                     /translation="MLDRNIRATVITNPMSGTLNVQAMLPGALAVLRAAGWQIDVQAT
                     TKSGDAGRLAAQARDQGQQVVLVAGGDGSLNEAANALAYSEVALGILPSGTANVWARQ
                     IGLPIPSPLRSDRLAEAARLLLDGAIRPIDLGRARDRYFVMWSGIGLDAEVTATIEPR
                     PVWVKRLGVISYSVHAIMTALSYRGTRMTVSVDAERIKCRALMVLISNAQLYGAIAQI
                     TPDARLDDGLLDIRILKGDGFFQAVRHMLRFLLRRRSPDPELIELRGQRISVTARRQC
                     YVHVDAEPFDRTPITIEVAPKALKMLIPRTAAASLFVNDS"
     gene            5532..7439
                     /locus_tag="VFF70_01300"
     CDS             5532..7439
                     /locus_tag="VFF70_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZY43358.1"
                     /translation="MSDYVLNNRYRLLSQVASGGMAVVYKAHDTVLNRVVAVKILREM
                     FAEDANFRARFQTEAQSAANLTHPNIVTVYDFSQDGSRTYIVMEYIEGRDLKSVIRSE
                     APLSLDRALDLFVQACAGLGYAHRAGLVHCDVKPQNMLVTNDGHLKVTDFGIARAISQ
                     IVPRDVETVWGTPHYFSPEQATGDTPTPASDVYSLGVVLYEMLSGRLPFEGDSHTDLA
                     MAHLRDEPPPLTALNPTVPIQIEQIINKVMAKEPSNRYRTADQLGRIVVEYRRMAEQS
                     TGMQVPMPMPITTSSDPINRAPIAPEPIGRSRSTDWLAWILGTLAVIAVIGLIPLMFL
                     VVKAYSEPLPVKPTPKATPTLSSTVGQFGEQVLVPNLIGRTEKDAEQFATSAGLQIVL
                     GLERFDAQIPTGSVVDQDPSVGKLAARGSAVTIALSKGPQVSPVQNVLSFVFEDVADG
                     LKTYGWDIRLVEQYNPEPFHKILNQEPVAGVPLAAGQPLTLTVSGGTTITLQVNFANL
                     IELDSANLPADQLKRGDTVNVTLFWHAVHAIPTPYTVFVHLIGPAGLTQQIDTEPGGG
                     SNPTTGWPANAQIADQYSLQIPVTAPKGEYQLRLGLYPTGQPFSRLPVVDPGDTTADN
                     NSILIKTISVQ"
     gene            7601..9532
                     /gene="ftsH"
                     /locus_tag="VFF70_01305"
     CDS             7601..9532
                     /gene="ftsH"
                     /locus_tag="VFF70_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZY43359.1"
                     /translation="MNTARPRNNFVYLLIVVAIGAIIFTALRGSSPQLDDVTLSQLVT
                     AINAGQVDTISVNNDDIKITEVNDKIVTSRKDPSLPLPEQLKALGVLDSAWAKVKYGV
                     ESPPEWGTLLSTLSFLLPGLLVVGLIYFMFRQAQGTNNQALSFGKSRARMFTGEHPTV
                     TFDDVAGADEAKQDLAEIVEFLKEPEKFVSLGARIPKGVLLVGSPGTGKTLLAKAVSG
                     EAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEIDAVGRHRGAGLG
                     GSHDEREQTLNQILVEMDGFDTDTNVIVVAATNRPDILDPALMRPGRFDRRVVLDRPD
                     IKGREEIFKVHVRGKPLGEDIQLGVLAKSTPGFVGADIENMVNEAAILAARRNRKTIL
                     MADFEEAMERVMLGPERKSRIITPKEKKITAFHEAGHAVVMRALPNSDQVRKVSIIPR
                     GFAAGLTWSMPEDDSSHMTRSKLKDLLAGILGGRSAEELVFNELTTGASNDLEKATEI
                     ARSMVTRWGMSDKLGPLVYGKKEELVFLGKELGEQRDYSEAVAQEIDGEVRLLMAEAH
                     ETATRVLKENREKLDRVANTLMEIESLDTADFEAVWNGEQPKGPEKYDSTPAPKPNKP
                     APESPAPTGSGMQPTPVPA"
     gene            complement(9917..10237)
                     /locus_tag="VFF70_01310"
     CDS             complement(9917..10237)
                     /locus_tag="VFF70_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HZY43360.1"
                     /translation="MIDAIGKSIGAQMSVGTDLAMKNDRATGNIIPPIVIGAQKRIET
                     MKRLTARGIDVDFENSFAYADSITDLGLFEMAGNPRPVYPDAELAAQAKSKGWPIHGE
                     CVNE"
     gene            10158..11318
                     /locus_tag="VFF70_01315"
     CDS             10158..11318
                     /locus_tag="VFF70_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZY43361.1"
                     /translation="MARSFFIAKSVPTDICAPIDLPIASIISVNPVETRTVKCPYSPH
                     PQRDLHALGEYLIQRHRQAITAASMDGSAFAGALPFKWQTDFDFPLFNGWKYNQDGKE
                     YERNILVVIPGKNRGEAIVMGDHYDTAYMEDSYAKERGGDGARIAAPGADDNASATAT
                     LLQAAPIFLSLAQEGKLERDVWLLHLTGEEFPSDCMGARVFCQALIEKTLKLQIGTDQ
                     WIDLSATRVVGVLVMDMIGHNRDSAQNIFQISPGKTTASLHLAWQAHLANLIWNAESV
                     EWNQRADRRWPGQCSTDSLTLPEIARHLPLEGEVRTSDDPASSVFNTDVQIFSDIGAP
                     AILVMEDYDIDRTGYHDSKDTLANIDLDYGAALSAVCIETLARAALLPKMTP"
     gene            12070..12453
                     /locus_tag="VFF70_01320"
     CDS             12070..12453
                     /locus_tag="VFF70_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43362.1"
                     /translation="MQFEQPLGTGLLSGQPSKTVFTLDMVAYDGWTTPVTLTFDPANA
                     QVLGIVGLTRKPATETTVSTLIVAPAEQVYLVAVTTADTPLGMYLLYIDAQGGGKQQS
                     LDLRLSVGSGPISFTVYLPLVMKIF"
BASE COUNT         2967 a         3416 c         3592 g         2744 t
ORIGIN      
        1 gtattgcagg aagccttcgc cggctttcgc taagaataaa aggcccggtg gtctacgatt
       61 catttcaaat cctcctgggt gggcggatcc gatgaattgt agactaccgg gctaactaaa
      121 acactatagc ttgatggtca aagttgctgc ttcagttgcg gggataggat tcgaacctat
      181 gacctccggg ttatgagccc gacgagctac cactgctcta ccccgcgatg taaactaacg
      241 ttcagattgt accacccggc acaacatccg tcaaggaatt ttgctaacgc ggttccccga
      301 agaaagccgt ccaccattca ccgagaatat cgtttggcgc tgcgttacct gatgcggatt
      361 gcgtcgttga cgttgaagaa gtgagttgac tggcgaccgg tacgacggcg atctcgcccg
      421 gctgcaccat gcgggctaaa cgcgcccaat gttcgacata gctatctgag ttcacccacg
      481 cttgcagttt caatagcgcc tcttgccgtg cgtgctccgt agtgagttgg gcggatagcg
      541 cgacttgttg cgcgtcgagt tcgcggctct gcgccaggcg cgcgttgaat tcagccaaaa
      601 aaggcagaac aataatcgcg gcgatgatca gccacacttg cggtgaacgc cacacggccg
      661 gacgccgact gctggacatg ctgcacctgc ttcaaaaata aaatcccgga acggttctgg
      721 gatttaagtg agtgccgaag ctgggatttg aacccagatg aggaaacccc cactacgccc
      781 tgaacgtagc gcgtctgcca atttcgccac tccggcggtg actaaaatga ttttaccagt
      841 ttgatttgaa atggtcaagc aatcgatgtc atttagaggc gctgctgacg atcacgcgcc
      901 gcgtgatcac ataccaatcg gcaacggtgc ggaaccgatc ggcaggaaaa gcgatcgggc
      961 cgatctgcac atttgaaact ttttcgctga cggcataggt atagatcgga taatacaata
     1021 agatgcccgg catccgttcg agaaacatct cctgaaactt gcgatagaga tcgatccgtg
     1081 tggagatatt ggaggtgcga cgagcctgct ccaaaatctc gctgacatct cgatcgcgat
     1141 actgcgaata attctgaccg gcattagctt gcgtctcatc ccaaaacgag tacggatcgg
     1201 gatcgccttg cagctgcaca tcgatcaagg ccgcttgata attgcgcgga gctagatagt
     1261 tactggcgag gccggcttgc accggtacga ccgcggcttt gacgccgatc gcgagccatt
     1321 gctgcgcgat ggatttcgcg acggcgatat gtaccggatc gctgttgcag tagagtgtga
     1381 acgagagggg cacgccgttc ttcacgcgaa taggcggctc atcagaaact ggcgaaggag
     1441 tcggtacaat cacgagaccg gtatcatcgg tggccggacg tgaagcgctg gagaactgcc
     1501 aacccgcctt gctcaacaat tgtcgcgctt ggccgagatc gagcgcgggc ggctgtagat
     1561 cagagtggta agcccacgtg ccggggatga tcgggctgtt ggcgagtatc gcttgaccgt
     1621 gcagaatctc gtcgatcaat ttctgccgat cgattgaaat gaacaaggct tggcgcacat
     1681 cttgatcctg gaagaatggc acatcatcgc cttgattgag atagatcatc gtgtaagcat
     1741 ccaacggcgc gttgtataaa ttcaaagtcg ggatgtcgcg cacggcgggt agattggcaa
     1801 acggcacatg gcttaggcct tgaatctcgc ccgcgcgata agccgccacc atcgatgaga
     1861 agtccgggaa aaatttgaaa tcgattttgg cgatgaacgg ttgcttgccg tagtagtgtg
     1921 aattcgcggc cagcaaaata tgttgagatt ggcccgcttc cggcgatcca aacgtctcca
     1981 ccgcgaatgg ccccgtgccg attggttttt tgttgaaggg cgagtcggct aaatctttcg
     2041 cagcggtatt ccccaaaata tgtttgggca gaataccgat tgaggcgtat tgcagaaacg
     2101 cggccagtgg ttcttgcagg gtcatttgga ttgtgctgtt gtcgacgtgg ctgatgacaa
     2161 cggtatacca caacgccgcc aaatcgggtg agccgggata gttggggtct ttcagcattt
     2221 gaagtgtgaa gaccacatct tccgcggtaa cgggttgatt gtcctgccag cgcaggttcg
     2281 atcgcaaatg gaatacgtag gtcaaaccgt tgtccagaat gtcccagtga tcagccaaat
     2341 cgggttgagg caagccatgc tcatcgaagt gaaccaggcc gctaaagatc aatgcacaca
     2401 gatcgtgatc gacctcattg tattggcaca acaaaggatt gatgaactgc ggcgaaccga
     2461 cgatgccttc cacgtaggtg ccgccgggcg ctggttcgcg ggtggtggtg aaggccacag
     2521 ccaggtaagc cagcaggcca cccagcatca gcgcccccag aacgatcaat gcaacttgcc
     2581 aacgaatatg gcgaatcact gcacaatcct tatggattct ctactggaag taagaagttg
     2641 gttgatggca gttggaagag ccgatcatga tcctcgatcc aacatctaac ctcaaatttc
     2701 caacttctaa cgacaacgat actgcaaaac ccgatcgatg accagcggct taccaggtgg
     2761 gacaataaac agggtgttgc gatcttgtcg atcacagcac cccgttcaaa tggagcgatg
     2821 aagttcgtaa accgatcggg aagtgcacta gacggcaacg ctcatcacgt tgagaatggc
     2881 caagatgaag aacacaatcg cgagtaagac cgttgcgttg aagagtgtac gttcaatgcc
     2941 gcgccgggtg tgatagaccg gactgtcgcc gccgccgaac atgctgccgg ccgaggagct
     3001 gcgcgcttgc aagatcacaa tgatgatcaa gccgacggcc acgataattt gagcaatatt
     3061 cagaatattc tgcatgttca atctcctaac gtaaaatctg gctggaatta taacacgcgg
     3121 ccaagtctgg cgcaaacaat catcttcatg tagaggcgct gactctacac ggatggcaaa
     3181 aagaaaaagg ccagcgcaac aatggcataa acgatcagca aagtcgcgcc ttccagccag
     3241 ttcgattcac cgtccagtga caccagtgcg gagatcaatg aggcggcgat taagctgacc
     3301 agttcaaaac tattgaactc caatgttaat gggtgaccca tcaacaaact cacaaacact
     3361 aagacgggcg ccacgaacaa ggcaatctgc aaactggaac ccatgacaat ggataggctt
     3421 aaatccattt gatttttcag ggccacttgt accgctacga cgtgctcggc gacgttgccg
     3481 atgatcggca cgatgatgat gccgatgaaa aattcactca gacccaatgc gtgtgaaacc
     3541 ggctcaaccg tgctgaccaa aaactcgctc atcacgacga tggccgccgt ggccgcgatc
     3601 aggatgccga tcgattggcg tgtcgtccag cgcggcgcgg tgtgcgaagg ctggatggac
     3661 gtgtctgtgg cggcctcgtg tttgtcgcgc gatcgaatgg tatagacgat tgaaaggacg
     3721 tagatcaaca gcatggccgc agcgaccgtc aaactaagtt cttcaacacg gacttgatcc
     3781 ggctcgatgg cggcattgaa gaacgaggga atgctgagag cgatcaccgc tagaatcgcc
     3841 agcgtcgaat ccagcccggc gtggctccga ttgaatcttt gcagcccatg tttgagtccg
     3901 cccgccaaaa tactcgcgcc tccaacgagc agcacattgc ccaagatcga tccgatgatc
     3961 gacgctttga ccagatccaa caatccggcg cgaacggcga acagggtgat gatcaattcg
     4021 gcggcattgc ccagcgtggc attgagcagc ccgccaaagc gcggcccgat tttttcggcg
     4081 atcgcttcgg tggcttcacc taacagcgcg gctaatggaa cgaccgctaa cgccgaagat
     4141 ccaaagacaa tcagcggact ccagtgcagc agttcggcca gaatcgcgat cggaataaag
     4201 agcaacaata gtcgtaagcc acgcataaat tctctcctgg ttggttgtag gagcgaggca
     4261 ttccgcatcg atcgatgttg aattcgcaga ctgcccgatc gggtcgatat caaacacaaa
     4321 tcaatttggg aatgacttgc cctacgaatc attgacaaac aacgaggctg ctgccgtcct
     4381 cggaatcaac atctttagcg ctttcggcgc aacctcgatc gtgatcggcg tccgatcgaa
     4441 cggttcagca tcgacgtgca cgtagcactg gcggcgcgcc gtgacgctga tccgttggcc
     4501 gcgcagttcg atcaattccg gatcgggcga tcggcggcgc agcaagaaac gcagcatgtg
     4561 ccgcaccgct tgaaagaaac cgtcgccttt caagatgcga atgtccagca ggccatcatc
     4621 cagcctagcg tccggtgtga tttgagcaat cgcgccgtac agctgcgcgt tgctgattaa
     4681 gaccattagg gcgcggcact tgatgcgctc tgcatcaacg ctgaccgtca tgcgcgtgcc
     4741 gcgataactg agggcagtca tgatcgcatg cacactgtaa ctgatcacgc caagccgttt
     4801 aacccagacc ggtcgcggtt cgatcgtggc tgtcacttct gcatcgaggc cgatgcctga
     4861 ccacatcaca aaatatcgat cgcgcgcgcg gccgagatcg atcggccgga tcgctccatc
     4921 gagtaacaga cgggccgctt cggctaatcg atccgatcgc aggggcgagg gaatcggcaa
     4981 accgatttgc cgcgcccaaa cattcgctgt gccgctgggc aatataccaa gcgccacttc
     5041 gctataagcg agcgcattcg ctgcttcatt taaactgcca tcgccgccgg cgactaagac
     5101 aacttgttgg ccctgatcgc gcgcttgagc cgcgagacgc cccgcatcgc cggatttggt
     5161 cgtggcctgc acatcgatct gccagccggc tgcgcgcagc actgccaacg cgccgggcag
     5221 catggcttgc acgttaagcg taccggacat gggattggtg atcaccgtcg cgcggatgtt
     5281 gcgatcgagc atcctcaccc cctagcccct ggcacatcga caaaaggaaa aggcgtcgat
     5341 tggccagggg agttgagtga ggcgattata gtgtacagct ccatgaattg gcaactgcgc
     5401 atctttgaga aattaaggta aaatatgaca gatttcttag gctacaaaga cacacaggca
     5461 cactgagtaa ttagatacga tatggtgtcc gtgtgtctta gcggttaata tccacccatt
     5521 cgggatcatt catgtcggac tacgtcctca ataatcgata tcgcttgctc tcgcaggtcg
     5581 ccagcggcgg catggccgtg gtgtacaaag cgcacgatac ggtgctcaat cgtgtcgtcg
     5641 cggtgaaaat tttgcgcgag atgtttgccg aagacgcgaa tttccgggcg cgttttcaaa
     5701 ctgaagcgca atccgccgcc aatctcacgc atccgaatat cgtgacggtc tatgacttca
     5761 gccaggatgg atcgcgcacc tatattgtga tggaatacat cgaggggcgc gatcttaaaa
     5821 gtgtgattcg atcggaagcg ccgctgtcac ttgatcgggc gctggatctg ttcgtgcagg
     5881 cgtgtgcggg cttgggttac gcgcatcgcg ccggattggt gcactgcgat gtgaagccgc
     5941 aaaacatgtt ggtcaccaac gacgggcact tgaaagtaac cgacttcggt attgcgcggg
     6001 ccatctcgca aattgtgccg cgcgatgttg aaaccgtgtg gggcacgccg cattattttt
     6061 cgccggagca agcgaccggc gatacgccga ctccggctag cgatgtgtac agtctcggcg
     6121 tcgtattgta tgaaatgttg tccggccgct tgccctttga aggcgacagt cacaccgatc
     6181 tcgccatggc ccatctgcgc gatgagccgc ccccgctcac cgcacttaat cctaccgtgc
     6241 cgattcagat cgaacagatc atcaacaagg tcatggccaa agagccgtcc aatcgctatc
     6301 ggacggccga tcaactgggc cgcatcgtcg ttgaatatcg acgcatggcc gagcaatcga
     6361 ccggcatgca agtaccgatg cccatgccga ttacgacgtc gagcgatccg atcaaccgcg
     6421 caccgatcgc gcctgaaccg atcgggcgat ctcgttccac cgattggtta gcgtggattt
     6481 taggaacgtt ggcggtgatc gcggtgatcg gtctgatccc gttgatgttc ctcgtcgtga
     6541 aggcttattc ggaaccgttg ccagtgaaac ctactcccaa ggccacgccg actttaagtt
     6601 ccaccgtggg gcaatttggt gaacaagttt tagtgccgaa cttgatcgga cgcaccgaaa
     6661 aagatgcgga acaatttgcc acatcggcag gtttgcaaat cgttttgggg ctggagcgct
     6721 tcgatgcgca gattcccacc ggctcagtgg ttgatcaaga tccgtcagtc ggcaaactcg
     6781 ccgcgcgcgg ctcagccgtg acgatcgcct tgagcaaggg cccgcaggtt tcgcctgtgc
     6841 agaacgtgtt gagttttgtg tttgaagatg tggctgacgg actcaagacc tatggctggg
     6901 acattcgctt ggttgagcag tacaatccag aaccgttcca taaaattttg aatcaagaac
     6961 cagtcgccgg tgtgccgttg gctgctggtc agccgctgac cttgaccgtc agcggcggca
     7021 cgacgattac tttgcaggtc aactttgcca atttgattga gttggattcg gcgaacttgc
     7081 cggccgatca actcaagcgt ggcgataccg taaatgtgac tttgttctgg cacgcggtac
     7141 acgcgatccc cacaccgtat acggtctttg tgcatttgat tggccccgcc ggtttaacgc
     7201 agcagatcga taccgagccg ggcggtggat cgaaccccac gacgggttgg ccggcgaatg
     7261 ctcagatcgc cgatcaatac tcgctgcaaa ttccagtgac tgctccgaaa ggcgaatatc
     7321 aattacgcct cgggctgtat cccaccggtc agcccttcag ccgtttgccg gtcgttgatc
     7381 cgggcgacac aacggcggat aataacagta tcttgattaa gacgatcagc gttcagtagc
     7441 gtaggcccag cggctgttca cgctaggcca taccgagatc gatcgatccc tgtttttcag
     7501 tcctcttaca caaatctaat agtcaattca tttgaagtaa ggtataattt caaccgatcg
     7561 gcattcgcaa gcgatcaagc aagcagtctg gaggttttac gtgaatacag cacgtccgag
     7621 aaataatttt gtgtacttgt tgattgtcgt ggctatcggc gcgattatct tcacggcact
     7681 ccgcggcagt tcgccgcagt tagatgatgt gactttgtca caactggtca cggcgatcaa
     7741 tgccgggcag gttgatacga tcagcgtgaa taatgatgat atcaaaatta ctgaagtcaa
     7801 cgacaaaatt gtgacttcac gcaaagatcc gagtctgccg ctgccggaac agttgaaagc
     7861 tttgggtgtt ctggacagcg cttgggcgaa ggtcaagtac ggtgtcgaga gtccgccgga
     7921 atggggcact ttgctcagca cgttgagttt cttgctgccc ggtttactgg tggtgggttt
     7981 gatctatttc atgttccggc aggcgcaggg caccaacaat caagcgctgt cgtttggcaa
     8041 aagccgcgcg cgcatgttta cgggcgagca tccgaccgtg accttcgatg atgtggccgg
     8101 cgccgatgaa gcgaaacaag acttggcgga aattgtcgag ttcttgaaag agccggagaa
     8161 attcgtatcg ctcggcgcgc gcatccctaa gggcgtattg ctggtcggca gtccgggcac
     8221 gggcaagacc ttgctggcca aggctgtttc cggtgaagcc ggtgtgccgt tcttcagtat
     8281 cagtggttcc gaatttgttg aaatgttcgt gggcgtgggc gcttcgcgcg tgcgtgatct
     8341 attcgatcaa gccaagcggc acagtccgtg cattgtgttt gtcgacgaga tcgacgcggt
     8401 cggtcgtcat cgtggcgcgg gcttgggcgg atcgcatgat gaacgcgagc aaacgctcaa
     8461 tcagattttg gtcgagatgg acggctttga taccgatacg aatgtgatcg tggtggcggc
     8521 caccaaccgg ccggacattc tcgatccggc gttgatgcgg ccgggccggt tcgatcggcg
     8581 cgtggtgctc gatcggccag atatcaaggg tcgcgaagaa attttcaaag tccatgtgcg
     8641 cggcaaaccg ctgggcgagg acatccaact gggtgtgctg gctaaatcga ccccgggctt
     8701 cgtcggcgcg gacatcgaga atatggtgaa tgaagcggcg atcctggccg cgcgacgcaa
     8761 tcgcaaaact attttgatgg ccgacttcga agaagcgatg gaacgcgtga tgctggggcc
     8821 ggagcgcaag agccgcatca ttacgccgaa ggaaaagaag atcacggctt tccatgaggc
     8881 cggtcacgcc gtggtgatgc gcgcactgcc gaacagcgat caggtgcgca aggtatcgat
     8941 cattccgcgc ggcttcgcag cggggttgac gtggagcatg ccggaagatg acagctcgca
     9001 catgacgcgc tcgaagttga aggatctcct ggccggaatt ctgggcggac gctcggcgga
     9061 agagttggtc ttcaatgaac tgaccaccgg tgccagcaac gatctggaga aagcgaccga
     9121 gatcgcgcga tcgatggtga cgcgctgggg catgagcgac aaactcggcc cgctggttta
     9181 cggcaaaaaa gaagagctgg tctttctggg caaggaactg ggcgagcagc gcgattatag
     9241 cgaagcggtc gcccaggaga tcgacggcga agtgcgcttg ctcatggccg aagcacatga
     9301 gacggcgacg agggtcttaa aggaaaatcg cgagaagttg gatcgagtcg cgaacacctt
     9361 gatggagatc gaatcgctcg atacggcgga cttcgaagct gtgtggaatg gtgagcagcc
     9421 gaaaggcccg gaaaaatacg acagtacgcc cgcgccgaag cccaacaagc cggcgccgga
     9481 atcaccggca ccgacaggca gtggtatgca gcccacacct gttccggcgt aaccagtaca
     9541 cgcagcacca caaatgaaat cgagactgct cattgagcag tctcgatttt tttatgcagg
     9601 ggcgaggtca cctcgcgcct tacagttaga aattcttcag cgcgagcggc agatagatgt
     9661 tgtagttgat cgatgcatcg cctcagaagc cgccgttgag ttgagacaac ccgccgttcg
     9721 gcgatccggc atccggctga ccgatcgatc cgccgatggt acgcgtcagt gacgcttgac
     9781 aattcatggt acctgtcagt gatgctttac cattcatgga tacgatccac ctacactaaa
     9841 cgttgtgcca ccgccatcga tcgtatacca cgaaagatcg taactgccgc tttgcggaga
     9901 ttcgatgatt cgctgcttac tcgttgacgc attccccgtg gatcggccag cctttggatt
     9961 tcgcctgcgc ggcgagttct gcatccggat agaccgggcg cggattgccg gccatctcaa
    10021 acaaacccag atcggtgata ctgtccgcgt aggcgaatga gttttcaaaa tccacatcga
    10081 tgccgcgcgc agtcagtcgc ttcatcgttt cgatcctctt ctgcgcgccg atcacaatcg
    10141 gtggaatgat attgcccgtg gctcgatcgt tcttcatcgc caaatctgtt ccgaccgaca
    10201 tttgtgcacc gattgatttg ccaattgcat cgatcatctc ggtaaatcca gtcgagacca
    10261 gaacagtcaa atgtccttac agtccgcatc cgcagcgcga tctccacgcg ctcggtgaat
    10321 atctgatcca gcgccaccgc caggcgatca ccgcggccag tatggatggc agcgcgttcg
    10381 cgggtgcgtt gccgttcaaa tggcaaaccg attttgattt cccgctgttc aatggttgga
    10441 aatacaatca agacggcaaa gagtacgaac gcaatattct ggtggtgatt cccggcaaga
    10501 accgcggcga ggcgattgtg atgggggatc attacgacac cgcctacatg gaagactctt
    10561 atgcgaaaga gcgcggcggt gatggcgcac gaatcgccgc gccgggtgcc gatgacaacg
    10621 cctcggccac ggcgacttta ttacaagccg ctccgatctt tttgagcctt gcgcaggaag
    10681 gcaaattgga gcgcgacgtg tggctgctcc atctcacggg cgaggaattc ccctcggatt
    10741 gcatgggcgc gcgcgttttc tgtcaggcgc tgatcgagaa aacactgaaa cttcagatcg
    10801 gcactgacca gtggatcgat ttgtccgcga cgcgggtcgt cggcgtattg gtgatggaca
    10861 tgattggaca caatcgtgac agcgcgcaaa atatcttcca gatatcaccg ggcaagacca
    10921 cggcgtcgct gcatttggcg tggcaagccc atctggctaa cttaatttgg aatgcagagt
    10981 cagtggagtg gaatcagcgg gccgatcgtc gttggcccgg ccaatgcagc actgacagcc
    11041 tgacattgcc ggaaattgca cggcatctgc cactcgaggg tgaggtgcgc acttcagatg
    11101 atccggcgag ttcggtcttc aacaccgacg tccagatctt ctctgacatt ggcgcgcctg
    11161 ccatcttggt gatggaagac tatgacatag accgcactgg ctatcacgac tccaaagaca
    11221 cgcttgccaa tatcgatctc gactacggcg cggcgctgtc cgctgtttgc atcgaaacgc
    11281 tggcccgggc ggccttgctt ccgaaaatga cgccttgaca tcgaaaggtg cgggtcgcga
    11341 cccaccatgt gtctcccgcc agaaaggctt cagcgtcccc acccacggct gatcctcacc
    11401 tataaccata accgcagcag cagctattcg gagactaccg cttcgattgt cgcggagcgt
    11461 cgcgatggcc agtcactcga cgaccccctg aacgcatcgc ttgaattgtt cccgtcattg
    11521 aggaacccct cggggatcta catgctgaat gcagctggcc caatccggct ggtggagtgg
    11581 aagtggatcg accgaaatgg tcagtagatg attaattcat tgctttcaat tcaacacgag
    11641 ataacggggg tagaggaatc ccgctcttcc ctgattcatc cccccaaccg atgagggtat
    11701 atcatgacct gcgccggagg taagattatc aagctgactc atcatgactc tgaaagcatc
    11761 acgggatatt tttggctacc ataatccgtt taagcccacg aggccgccca accagtcgcc
    11821 cagggttctc acaaagtcag actgggtcgc tgaaaaagca gccgcaacgc ttgatcaggc
    11881 tgcgcctcgt aatagcgccg agcctgagct aaatgggtcc aacctcgccc gatcgtcagc
    11941 ccaataatca agttgttcag actggcgatc acctgaccga agcgtttgaa gattgttcga
    12001 cccgcatctt cacgtcaggt cacgtcccgg cgataatgta gatcattctc gatgccccca
    12061 gtgcctgcga tgcaattcga gcaaccgctc gggactggcc tgttgagcgg tcaaccgagc
    12121 aagaccgtgt tcacgctgga catggtcgcc tacgatggtt ggacaacacc ggttacactc
    12181 acgttcgatc cggccaacgc tcaggtctta ggcatcgtcg gcttgacgcg caagcctgcc
    12241 accgagacaa ccgtcagcac attgatcgtt gcgccagccg agcaggtcta tctcgtcgcc
    12301 gtcaccacag cggacacgcc gttagggatg tatctactgt acatcgatgc gcagggcgga
    12361 ggcaagcagc agtcgctgga tctccgcttg agcgtcggca gtggcccaat atcgttcacg
    12421 gtgtatctgc cgctagtgat gaaaattttt tagattaagg ccgtaaggca gtcagaagat
    12481 aacgtaaaaa aagaggccgc gccttcatgc acggtctctt tttggttcca ctgtctagat
    12541 caccaccagc ttgtgcaagg gtctgcagcg gtggtttagt ggagcctgtc tcgttaatca
    12601 atttatcaac gtaacgactt atcggcgctc agagatctgc atcgacacac agccaaacga
    12661 ggcgtggaga ggttgctgat ttataacgta atagaattgg agaaagaggt gcttggtgg
//
LOCUS       DASRTJ010000071         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_231003,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000071 DASRTJ010000000
VERSION     DASRTJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231003"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="rfbH"
                     /locus_tag="VFF70_01325"
     CDS             complement(<1..992)
                     /gene="rfbH"
                     /locus_tag="VFF70_01325"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HZY43363.1"
                     /translation="MGREDADRRRVEGNDSMDAEVNDRDSKQKRAADLREEILAKVAE
                     YYQLKHADRSFTPGESLVHYAGRVFDEHELINGVDAVLEFQLTHGRFGREFEKKLGAF
                     LGMREVIPVNSGSSANLIAVSTLCAQQLHNGLRPGDEVITPAVTFPTTLAPIIQNRLI
                     PVLVDCQLGNYNIDVDQLEAAYSNKVRAMFIPHTLGNPVEMDKVIEFAKKHELFLIED
                     TCDALGSTYDGKLCGTFGHLATLSFYPAHHITMGEGGAVYTHSRRLARLARVMRDWGR
                     DCFCGYDNPADGKCGHRFDWKIDGSDEPYDHRYLYTEIGYNLKPTDLQASIAVA"
     gene            complement(929..1798)
                     /locus_tag="VFF70_01330"
     CDS             complement(929..1798)
                     /locus_tag="VFF70_01330"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY43364.1"
                     /translation="MHYLITGATGFIGSHLTRRLVGLGERVSIFIRSNSDRSAIKAVL
                     EKLDMHEVDLRTASAVSRAIDMIEPTIVFHCAAAGVSDPFLPPDDAIRSNVYGTINLL
                     RAIKGRAPVIVLRTPGEIEALNVYAAAKAAAWQFCRMYRRTQGWPIAGAMVYQVYGPH
                     QDRKNVIPSAIEAALNDRDFAITSGRSQRDWIYIDDVIDALIAQAQADRFDVDSIDLG
                     TGSTLSVRAVVERIYQLIGRGGKPIVGALPDRPGEVDIQKADVTTAASILNWDAKTPI
                     DDGLKATIQWMQK"
     gene            complement(1830..2819)
                     /locus_tag="VFF70_01335"
     CDS             complement(1830..2819)
                     /locus_tag="VFF70_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY43365.1"
                     /translation="MAVDKFWKNKKVFVTGCTGIVGSWLTLALLDRGADVIGLIRDRV
                     PQSNLMLSGAIDRIVGVDGDITDQALLERILNEYEIEIVFHLAAQPLVRMANRAPIGT
                     FETNIRGTWLLLEACRHSPTVKRIVLASSDKAYGDQDKLPYTEEAPLQGRHPYDVSKS
                     AADLIAQAYACTYDLPVAITRCGNIYGGGDLNWDRIVPGTIRSVLLGEAPIIRSDGTP
                     LRDYLHVDDIVDGYLRVAEKLDDRSMYGQAFNFGMDDPRSALDIARLIISISDRSDLK
                     PVVLNQASNEIGKQYLASEKAHRVLGWKAQYSLEAGLRKTLQWYREFLMHNAS"
     gene            complement(2897..3673)
                     /locus_tag="VFF70_01340"
     CDS             complement(2897..3673)
                     /locus_tag="VFF70_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HZY43366.1"
                     /translation="MPVVILCGGSSTRMRGSSGDDRPKPLVEVGDQPVVWHVMKIYAH
                     YGYTDFVLALGYRGEMIKNYFLDYLPMSRDVTLNLGETRDVTYHAHNDEEQWRVTLTD
                     TGTETQTGARIKKIERYIDTPTFFATYSDGVGDIDLNALLKFHRAHGKLATVTGVRPF
                     SQFGLIETDGDRVTGFREKPQVEGHVNGGFFVFERGVFDYLRGPDTLELEKEPLVNLA
                     RDGQLMIYRHDRFWRAMDTLKDAQVLNDLWKNGSAPWKIW"
     gene            complement(3816..4127)
                     /locus_tag="VFF70_01345"
     CDS             complement(3816..4127)
                     /locus_tag="VFF70_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43367.1"
                     /translation="MAETRVKMLVSYDVVAELQQGYYEFVLGEMIPTAQSMGLQLSEA
                     WHTAYGNYPIRLNGFVANDRAALDRILATPEWKTLEEKLKRFVTDYQRKIIPYREGFQ
                     F"
     gene            complement(4138..>4654)
                     /locus_tag="VFF70_01350"
     CDS             complement(4138..>4654)
                     /locus_tag="VFF70_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZY43368.1"
                     /translation="DYIQPYIVGYKIDLKSMRDKNYRQLGAVMNNVLDGIKLVHERGI
                     WLEIVTLIIPGFNDSNEEIWDAARYIASVSKDIPWHVTAFHKDYKMTDPDNTDARTLL
                     RAAEIGQEAGLNYVYSGNLPKQVGSYENTYCPHCNELLIARQGYRILANKLAGRGICP
                     KCGTTIPGIWK"
BASE COUNT         1093 a         1369 c         1124 g         1068 t
ORIGIN      
        1 tgcgccaccg cgatcgaggc ctgtaaatca gtcggtttta agttgtagcc gatctcggta
       61 tacaggtaac gatgatcgta aggctcatcg ctgccatcga tcttccaatc gaaacgatgg
      121 ccgcatttcc catccgccgg attatcgtag ccgcagaaac aatcgcgacc ccaatcgcgc
      181 atcacgcgcg cgagccgcgc cagccggcgg ctgtgcgtgt agaccgcccc gccctcgccc
      241 atcgtaatat gatgcgccgg ataaaaactc aacgtcgcca aatgaccgaa cgtaccgcac
      301 aatttgccat catacgtgct acccagcgca tcgcaggtgt cttcgatcaa aaataattcg
      361 tgcttctttg caaactcgat aaccttgtcc atctcgacag gattgcccaa ggtatgcgga
      421 ataaacatcg cccgaacctt gttggaatac gctgcttcca attgatcaac gtcgatgttg
      481 taattgccta attggcaatc gaccaaaacc ggtatcaatc gattctgtat gatcggcgcg
      541 agcgtcgtcg gaaaagtgac cgcgggcgta atcacttcat cgccgggccg cagcccgttg
      601 tgcaactgct gcgcacacaa agttgacacg gcaatcaagt tggccgacga tccggaattc
      661 accgggatca cttcacgcat gcctaaaaac gcgccaagct tcttttcgaa ctcgcggcca
      721 aagcggccgt gtgtcaattg aaattccagc accgcatcga cgccgttaat caactcatgc
      781 tcatcgaaca cgcggcccgc ataatgcacc aacgattcgc ccggcgtgaa cgatcgatcg
      841 gcgtgcttga gctgataata ctcggcgacc ttcgccaata tttcctcgcg caagtcggcc
      901 gcgcgttttt gttttgaatc tcgatcgttt acttctgcat ccattgaatc gttgccttca
      961 acccgtcgtc gatcggcgtc ttcgcgtccc aatttaagat cgatgcggct gttgtcacat
     1021 ccgctttttg gatgtccact tcgcccggcc gatcaggcag cgcacccacg atcggtttgc
     1081 caccgcgccc aatcagctga taaattctct cgactactgc gcgcacactc aacgtgctgc
     1141 ccgtgcctag atcgatcgaa tccacatcaa accgatcggc ttgggcttgc gcgatcaagg
     1201 catcgatcac atcatcgatg tagatccaat cgcgctgtga tcggcccgag gttatcgcaa
     1261 aatcccgatc gttcagtgct gcctcaatgg ccgaggggat aacattcttt cgatcttgat
     1321 gcgggccata gacttgatac accatcgcac cggcgatcgg ccagccctgc gtgcggcgat
     1381 acatgcgaca gaactgccac gcggctgctt tggccgccgc ataaacattc aacgcctcga
     1441 tctcgcccgg cgtccgcaaa acaatcacgg gcgcgcggcc tttgatggcg cgcagtaaat
     1501 tgatcgtgcc atagacattc gagcgaatgg catcatccgg cggcaaaaac ggatcgctca
     1561 caccggcggc cgcgcaatga aaaacgatcg tcggctcgat catatcgatc gcacgcgaga
     1621 cagcgctggc cgtccgcaga tcgacttcgt gcatatccag tttttcaaga acggctttga
     1681 tcgcagagcg atcggagttg gatcgaataa aaatactgac gcgctcgccc agtcctacta
     1741 atcggcgcgt cagatgcgag ccgatgaagc ctgtcgcgcc ggtgattaga taatgcactg
     1801 cttgtcaccc ttcgcgtctc agcatgacat caacttgcat tgtgcattaa gaattcacgg
     1861 taccactgca gcgtcttgcg cagacccgcc tcgagtgaat actgtgcttt ccagcctaat
     1921 acgcgatgcg ctttctcgct cgctaaatac tgtttgccaa tctcgttgct tgcctgattc
     1981 aatacgactg gcttgaggtc ggaccgatca gagatcgaaa taatcaatcg ggcaatatcc
     2041 aaagctgatc ggggatcgtc catgccaaag ttaaaagcct gaccatacat cgatcgatcg
     2101 tccaattttt cggccacgcg caaataccca tcgacaatat catcgacatg caagtagtcg
     2161 cgcagcggcg tgccatcact gcgaatgatc ggcgcttcac ccagtaacac actgcgtatc
     2221 gtgccgggca caatgcgatc ccaattcaga tcgccgccgc cgtaaatgtt gccgcagcgc
     2281 gttatcgcca cgggcagatc gtaggtgcag gcatacgctt gcgcaatgag atccgccgcc
     2341 gactttgaga catcgtacgg atggcggcct tgcagcggag cctcttcggt gtacggcaac
     2401 ttgtcctgat caccatacgc tttatccgaa gacgccaaga cgatgcgctt cacggtgggt
     2461 gaatgtcgac aggcttccag taataaccac gtgccgcgga tattggtctc gaatgtgccg
     2521 atcggcgcgc gattcgccat tcggacaagc ggttgagccg caagatggaa aacgatctcg
     2581 atctcatatt cattcaaaat acgttcgagc aacgcctgat cggtgatatc gccatcgaca
     2641 ccaacaatgc gatcgatcgc accggacaac atgagattag actgcggcac tcgatcgcga
     2701 atcaagccga tcacatctgc gccccgatcg agcagtgcga gtgtcagcca cgatccaacg
     2761 ataccggtgc agccggtaac gaagactttt ttgtttttcc agaatttgtc tacggccaag
     2821 tgaaccttct tcaactcggt gcatactgca taattctaaa ctacgcattg tgcattaaga
     2881 attatatctt cagcatttac caaatcttcc acggcgcgct gccattcttc cacagatcat
     2941 tcagcacttg cgcatctttt agcgtatcca tcgcgcgcca gaatcgatcg tgccgataga
     3001 tcatcagctg tccatcgcgc gctaaattca ccagcggctc tttctctaat tcgagcgtgt
     3061 ccgggccgcg taaataatcg aacacgccgc gctcgaacac aaagaaaccg ccgttgacat
     3121 gcccttccac ttgcggcttc tcgcggaagc ccgtcacgcg atcgccgtcg gtttcgatca
     3181 agccgaattg cgagaatggc ctgacgcccg tgacggtcgc cagcttgcca tgcgcccgat
     3241 gaaatttcag cagcgcattg agatcgatgt cgcctactcc gtcgctataa gtagcgaaaa
     3301 acgttggggt gtcaatatac cgttcgatct ttttgatgcg cgcgcccgtc tgtgtttccg
     3361 tcccggtatc cgtcagtgtc acacgccact gctcttcatc attgtgcgcg tgataggtca
     3421 catcgcgcgt ctcgcccaga ttcagcgtga catcgcggct cattggcaga taatccagga
     3481 agtaattctt gatcatctca ccgcgatagc cgagcgccag cacaaagtcg gtgtagccgt
     3541 aatgcgcgta aatcttcatc acgtgccaga ccaccggctg atcgccgacc tcgaccagcg
     3601 gcttcggccg atcgtcgccg ctggagccgc gcatccgcgt gctggagcca ccgcataaaa
     3661 tcactaccgg gattcgatcg ggcatgagca ggctgctttc aaaaaataat ctttgatcgc
     3721 cgacacatca aaccacatcg gtcagcgaca aagattgaac ggtgcagatt atatcataca
     3781 tcaagccgcg ttcggatcga tcacgcgctc aaccgttaaa actgaaagcc ttctcgatac
     3841 ggaataattt tgcgttgata atccgtgaca aaccgcttga gtttttcttc gagggtcttc
     3901 cactccgggg tggccaggat ccgatcgagt gcagcacgat cgttggcgac aaaaccgttg
     3961 aggcgaatag ggtaattgcc ataagccgtg tgccaggctt cactcaactg caggcccatc
     4021 gattgggccg ttggaatcat ctcgcccaat acaaattcat aatagccctg ctgaagttca
     4081 gccaccacat cgtacgagac taacatcttc acgcgtgttt cagccataat cgatcgatca
     4141 cttccatatg ccgggaatgg ttgtgccgca tttcggacaa attccacgcc cggccagttt
     4201 gttcgccaaa atccgatagc cctgtcgagc gatcaagagt tcattgcaat gcggacaata
     4261 agtattctcg tatgaaccaa cctgtttagg taaattgcct gaatacacat agttcagtcc
     4321 ggcctcttgg ccgatctccg ccgcgcgcag caacgttcgc gcatccgtgt tatcgggatc
     4381 agtcatcttg taatctttgt ggaaggccgt cacatgccac ggaatatctt ttgagacaga
     4441 ggcaatatag cgagccgcgt cccatatctc ttcattgcta tcgttgaaac cgggaatgat
     4501 caacgtcaca atttccagcc agatgccgcg ctcgtgaacc agcttgatcc catcgagcac
     4561 gttgttcatc accgcgccca actgtcgata attcttatcg cgcatcgact tgagatcgat
     4621 cttgtagccg acgatgtacg gttggatgta atcc
//
LOCUS       DASRTJ010000072         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_231069,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000072 DASRTJ010000000
VERSION     DASRTJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231069"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /gene="gltX"
                     /locus_tag="VFF70_01355"
     CDS             complement(<1..1395)
                     /gene="gltX"
                     /locus_tag="VFF70_01355"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZY43369.1"
                     /translation="MTVRVRFAPSPTGFLHVGGLRTALFNWLFVRKMGGQFILRIEDT
                     DQKRYAPGGVRAIMDGLLWLGLQWDEGPDKASLRLMQQNEDWDGAPDIGGPHGPYVQS
                     LKVKRFQEAAEELIAKGWAYRCDCPPDRLAQVRKEQIDRKQPARYDRRCRFKQAGDVD
                     PTKPHVVRLAMPLEGTTTVNDLIRGSIMVDYKLLDDFIIIKSDGLPTYHLAAMVDDHD
                     MQITHVLRGDEWLPSLPRHLLIYTAFGWTPPIFAHLPVILNPSGKGKMSKRSAKAAGG
                     DFENSVFVTDFRDEGYLPEAMRNFLALVGWAPGSGIEQEIFTLDDLINVFSLDHVNPS
                     PAAFPYDKLDWMNGMYIRALPIDELARRVYPFLLNAGLEVDTDRLMKIAPDIRERMVT
                     LKDAVEATDFLFTTTVTPDPAQLVGKELTPGQTLDILKQAEEYVRTCEPFEAELMEHA
                     LRDLAEKSGLRLGAF"
     gene            complement(1493..2311)
                     /locus_tag="VFF70_01360"
     CDS             complement(1493..2311)
                     /locus_tag="VFF70_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43370.1"
                     /translation="MLRTPQSRRGTTRRAVCFIALIGLTVAACNNREVPTARPSSVPT
                     VAVSISVVPSLVITLTPQPVFIFTDEFTAYTLSGTIYDAVAGLAADLPQAHLSWHFAA
                     PALQQFNGQTVADANGIYRIPIRVRLKDEVQLTASAPGYLPTTVRLHAREIALNGERL
                     DFGLVKANGLWPTVPGDLGSLSVIGIVYDAYRGQLSPIGGAAVTITLISIVHPPSLIV
                     LITPTDGSFNTTLLMHSTDQIKVVASANDYRETYHLYAASTLTQSLPLLIALSH"
     gene            complement(2317..>2570)
                     /locus_tag="VFF70_01365"
     CDS             complement(2317..>2570)
                     /locus_tag="VFF70_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZY43371.1"
                     /translation="FLLYRLIRIADRSRDQYGQLVVIGFATMIFFQSAINIAMNLNMG
                     IVAGLPLPFISYGGSSLLTLLIAIGVALSVVMRHKKIEF"
BASE COUNT          601 a          697 c          714 g          558 t
ORIGIN      
        1 gaacgcgccg agtctcaagc cggacttctc ggccaaatca cgcagcgcat gttccatcag
       61 ctcggcttca aagggctcac acgtgcgcac atactcttca gcctgtttca agatgtcgag
      121 cgtctgccct ggcgtcagct ctttgccaac gagctgcgca ggatcgggcg tgaccgtcgt
      181 ggtgaacaaa aagtccgtcg cctcgacagc atctttcaac gttaccattc gctctcgaat
      241 atccggtgcg atcttcatta agcgatcagt atcgacttca agcccggcgt tcagcaagaa
      301 tggatacacg cgccgggcta actcatcgat gggcagggcg cggatgtaca tgccgttcat
      361 ccaatcgagt ttatcgtatg gaaatgctgc gggtgaagga ttgacgtgat ccaagctgaa
      421 gacgttgatc aagtcatcca gcgtgaaaat ttcctgctcg ataccggaac ccggcgccca
      481 accgacgagt gccagaaagt tgcgcatcgc ctccggcaaa taaccctcgt cgcgaaaatc
      541 agtcacgaat actgaatttt caaaatcacc ccctgcggct ttggccgatc gcttgctcat
      601 cttgcctttg ccgctgggat tcaaaatcac cggcagatgc gcgaagatag gcggcgtcca
      661 accgaaagct gtatagatca aaaggtggcg cggcaaactc ggcagccatt catcgccgcg
      721 cagcacgtgg gtgatctgca tgtcatgatc gtccaccatc gctgcgagat gataggtagg
      781 caagccgtct gatttgatga tgatgaaatc gtcgagcagc ttgtaatcga ccatgatgct
      841 gccgcgaatg agatcgttga ccgtcgttgt gccttcgagc ggcatagcca aacgcacgac
      901 atgcggctta gttggatcga catcgcccgc ctgcttgaag cggcagcgtc gatcgtacct
      961 cgccggttgt ttgcgatcga tctgctcttt acggacttgc gctaatcgat cgggtgggca
     1021 gtcacaccga tatgcccaac cttttgcaat gagttcttca gcggcttctt gaaatcgttt
     1081 tactttcagc gattgcacat atggcccatg cggtccgccg atgtccggcg cgccatccca
     1141 atcttcattc tgctgcatca gacgcaggct ggctttatcc gggccttcat cccattgcaa
     1201 accgagccac aataaaccat ccatgatcgc gcgcacgccg ccgggtgcgt agcgtttctg
     1261 atcggtgtct tcgatgcgca gaataaactg gccgcccatt ttgcgcacga ataaccaatt
     1321 gaacaaagca gtgcgcaaac cgcccacatg caggaaaccg gtcgggctgg gcgcaaaacg
     1381 aacacgaact gtcataggtg ctccgtcaaa atgttggtta aagagagctg gttctcaagt
     1441 taatgcatga tccgagatcg atgaccagtg atggatgaat gcgattgtgc ctttagtgcg
     1501 ataaggcaat aagcagcggc aaactctggg tgagtgtact cgcggcgtac agatgatatg
     1561 tttctcgata atcattcgcg cttgcaacca ctttgatttg atcggtcgag tgcatcaaca
     1621 atgtggtgtt gaacgatccg tcagtgggtg tgatgagcac gatcagtgac ggcggatgca
     1681 cgatcgagat cagcgtgatc gtaaccgcag cacccccgat cggactgagc tgaccccgat
     1741 aggcatcgta cacaattccg atcacgctca acgaccctag atcgccgggc accgttggcc
     1801 acaagccgtt ggccttgact aaaccgaagt cgagccgctc cccgttcagt gcaatttctc
     1861 tggcatgcaa gcggacggtc gtaggcaaat acccgggcgc gctggccgtt aactgcactt
     1921 catcttttaa ccggacgcga attggaatgc gatagatgcc gttcgcgtcg gcaactgtct
     1981 ggccgttaaa ctgctgtaga gcaggcgcgg caaaatgcca actcaagtgg gcctgcggca
     2041 gatcggcggc cagtccggcg accgcatcat agatcgtacc gctcagggtg taagccgtaa
     2101 actcgtctgt gaagatgaag actggttgcg gcgtgagcgt gataacaaga gatggtacga
     2161 cgctgatact tacggcaacg gttggcacac tcgatggacg ggcggtgggc acttcgcgat
     2221 tgttgcaggc ggcaacggtc agtccgatca gcgcaatgaa acaaacggcg cgccgtgtcg
     2281 tgccgcgtcg gctttgagga gtccgcagca tgtgcgtcag aactcgatct ttttgtgacg
     2341 catgacaacg ctaagcgcca caccgatggc gatcaatagc gttaagagcg agctgccgcc
     2401 ataactgata aacggcagtg gcaggccggc gacgatgccc atgttcaagt tcatggcaat
     2461 gttgatggcc gactggaaaa agatcatggt ggcaaagccg atgactacca gctggccata
     2521 ttgatcgcgc gatcgatcgg cgatgcggat taatcggtag agcagaaatg
//
LOCUS       DASRTJ010000073         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_231142,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000073 DASRTJ010000000
VERSION     DASRTJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231142"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3262)
                     /locus_tag="VFF70_01370"
     CDS             complement(<1..>3262)
                     /locus_tag="VFF70_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43372.1"
                     /translation="PYIIEKGERIRIEADPLGDKGFAPHIMRTKQALMFNTEVEARSK
                     EYGSYVIGGEASKSYLGVPLISGNEARGIIALQNVDRENAFTDSDLSLLTTLSASMSV
                     ALENARLFDETTRLLKETDQRAAELALINSVQEGLASKLDVQSVYDLVGDKIRDIFDA
                     QVVQIMAYDRDANRCYWRYMIEKGERQYTDPTEPRGFSSYILKNAQPLMLNEGVNGWW
                     REHLGTPPTVLAGEMAQSYLGVPLIIGAEARGVISLQNVDREHAFAESDLRLLLTLAS
                     SMSVALENARLFDAERQRAQELEIINSVGEALASQLDMQAVYDLVGDKIRSIFDAQAV
                     TIATYDRETNLIHYPYMIERGQRQQQAPLPITQRGFSAHIMQTRQPLMINQETAQRAV
                     EYGSTIIGGGEMAKSYLGVPLVIGGEARGVISLQNIDRENAFSDSDLRLLTTLSSSMG
                     VALENARLFAETNRRASEMSALTDIGREISSTLDLEAVIDRIAFRAQEVLRAGTSAVY
                     LLEPDGITLRPIAAVGDYSHEVLTSRSRLGEGIIGGVAQSGVAESITDTAQDPRTVHM
                     AGTPEEAHGEKMMVAPLFARDQVIGAMAVWRDAGNSLFAADDLGFLVGLSRQAAIAIQ
                     NARLFEESQKRANETAALNEIGREISATLDINVVLERITQSARDLLIGDTSAVYLVQP
                     DGETLKAVVAIGDIVEQIKASAPKLGTGIVGSIAQTGVPEFINNTVLDRRAVHIAGTS
                     NDDAGKKIMVAPLPAKDRILGVMAVWRDVDRPLFTQTDLDFLTGLARQAVIAIDNARL
                     FDEVQQQKQYSESLVQYSPVAIVTSDLNNIVVSWNPGAEKLFGYKPAEAVGRNLDELI
                     TTAEQRVEAKKYSQQTMLGERIHAITRRLRKDGSFVDVELSSVPVKMGGSGPRSYVAI
                     YHDITELQRAKQEAESANSAKSAFLATMSHEIRTPMNAIIGMSGLILDTPLNGEQREF
                     AEIIRNSGDALLMIINDILDFSKIEAGKMDMESQPFDLRECLEGTLDLIATRAYDKGL
                     DLAYMIDEHVPPTILGDITRVRQIVLNLLTNAVKFTEKGEVVLDVKPEGSIGPNQKS"
BASE COUNT          691 a         1008 c          856 g          707 t
ORIGIN      
        1 gatttctgat tcggcccgat cgatccttcc ggcttaacgt ccaaaaccac ttcgcccttt
       61 tccgtaaact tcacggcatt cgtcaacaga ttcaacacaa tctgccggac acgcgtgatg
      121 tcgccaagaa tcgtcggcgg cacatgctca tcgatcatat aggccagatc gagtccctta
      181 tcatacgcgc gggtggcgat gagatcgagt gtgccttcca ggcactcgcg cagatcgaac
      241 ggctggcttt ccatatccat cttgcccgct tcgatcttgc taaagtccag aatgtcattg
      301 atgatcatca gcagcgcatc gccggaattg cgaatgatct ccgcgaactc gcgctgttcg
      361 ccattcaacg gcgtatccag gatcaagccg ctcatgccga tgatcgcatt catcggcgtg
      421 cggatttcgt ggctcatcgt cgccaggaac gcgctcttgg ctgaattcgc cgattcggct
      481 tcctgcttgg cgcgttgcag ttcggtgatg tcgtgataga tcgccacgta actgcgcgga
      541 ccgctgccgc ccatcttcac cggcaccgat gagagttcca catccacaaa gctgccatct
      601 ttgcgcaggc ggcgagtaat ggcgtgaatg cgctcgccca gcatggtctg ctgcgaatat
      661 ttcttcgctt ccacgcgctg ctctgccgtc gtgatcaact cgtcgagatt gcggccaacg
      721 gcttcagccg gtttgtaacc aaacaatttt tccgcgcccg gattccacga cacgacgatg
      781 ttgttcagat cgctggtgac aatcgccacc gggctgtact ggacaagcga ttccgaatat
      841 tgtttctgct gctgcacttc atcaaacagg cgcgcattgt caatagcaat cacggcttgc
      901 cgcgcgaggc cggtgagaaa atccaagtcc gtctgtgtga acaacggccg atcgacatcg
      961 cgccagacag ccatcacacc gaggatgcga tctttcgccg gcagcggcgc gaccatgatc
     1021 ttcttgcctg catcgtcatt cgatgttcca gcgatatgca ccgcgcgccg atcgagcacg
     1081 gtgttgttga taaattccgg cacgccggtc tgcgcgatgc tgccgacgat accggtgcct
     1141 aacttcggcg cgctggcttt tatttgctca acaatatcgc cgatggcgac cacggccttc
     1201 aacgtttcac catccggttg aacaagatac actgcactgg tgtcgccgat caacaaatcg
     1261 cgcgcgctct gggtgatgcg ttccaacaca acattgatat ccagcgtggc cgaaatttca
     1321 cggccaattt catttaaggc cgccgtttca ttggcgcgtt tctgcgactc ttcaaacaag
     1381 cgcgcatttt gaatggcaat cgcggcttga cgcgataaac cgacgaggaa gcccagatca
     1441 tcggcggcaa acaaactgtt accggcgtcg cgccagaccg ccatcgcacc gatgacttga
     1501 tcgcgcgcaa acaacggcgc gaccatcatc ttctcgccat gcgcttcttc cggcgtgccc
     1561 gccatatgca ccgtgcgcgg atcctgcgcg gtatcggtga tcgattcagc cacaccgctc
     1621 tgggccacac cgccgatgat accttcaccc agccgcgagc gcgaggtaag gacttcgtgt
     1681 gaataatcgc cgacggccgc gatcgggcgc agcgtgatcc cgtcgggctc cagcaaatat
     1741 accgcgctgg tgccggccct caacacttcc tgcgcgcgga aggcgatccg atcgatcacg
     1801 gcttccagat cgagcgtgga cgaaatttcg cggccaatgt cggtcaacgc ggacatttca
     1861 ctcgcgcgcc gattagtctc ggcgaacaaa cgcgcattct ccaacgcgac gcccatggat
     1921 gaagacagcg tggtgagcag ccgcagatca gaatcgctga acgcgttttc ccgatcgatg
     1981 ttctgcagcg agatgacgcc cctcgcctcg ccgccgataa ccagcggcac gccgagatac
     2041 gacttcgcca tttcgccacc gccgatgatg gtcgaaccat actccactgc ccgctgggca
     2101 gtctcttgat tgatcatcaa cggctggcgc gtctgcatga tgtgcgcgga aaaaccgcgc
     2161 tgggtgatgg gcagcggcgc ttgctgttgc cgctggccgc gttcgatcat gtacgggtaa
     2221 tggatcagat tggtctcgcg atcatacgtg gcgatcgtga ccgcctgcgc gtcgaagatc
     2281 gatcgaatct tgtcgccaac caaatcatag acagcctgca tatccaactg tgaagccagc
     2341 gcttcaccga cactgttgat gatctctaat tcctgcgcgc gctggcgctc cgcatcaaac
     2401 aagcgggcgt tctccagggc tacactcatt gaggaagcca gcgtcaacaa caggcgcaaa
     2461 tcggattcgg caaaggcatg ctcccgatcg acattctgca gcgagatgac gccgcgcgct
     2521 tcagcgccga tgatcaacgg cacaccgaga taggattgag ccatctcacc ggccaggacg
     2581 gtcggtggtg tgccgagatg ctcgcgccac cagccattca caccttcatt caacatcaac
     2641 ggctgggcgt tcttgaggat gtaactggag aaaccgcgcg gctcggtggg atcggtatac
     2701 tgccgctcac ctttctcaat catgtaacgc caataacagc gattcgcatc tcgatcatac
     2761 gccatgatct gcacgacctg cgcgtcgaag atgtcgcgaa tcttgtctcc gaccagatcg
     2821 taaaccgatt gcacatccaa cttcgaagcg aggccttcct gcacactgtt gattaacgcc
     2881 agttcggccg cgcgctgatc ggtttctttc agcaaacgcg tggtctcgtc gaataggcgc
     2941 gcattctcca aggcgacact catcgacgca gacaacgtcg tgagcaaact cagatcagaa
     3001 tccgtaaagg cattctcgcg atcgacattt tgcagggcta taatgccgcg cgcttcattg
     3061 ccgctgatca gcggcacacc cagataggat ttagaggctt cgccgccaat cacgtacgag
     3121 ccatattctt tagagcgcgc ttccacctcc gtgttgaaca tcaacgcctg cttggtgcgc
     3181 atgatgtgcg gcgcaaagcc tttgtcgccc aacggatctg cctcgatacg gatgcgttcg
     3241 cccttctcga taatatacgg at
//
LOCUS       DASRTJ010000074        10502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_231157,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000074 DASRTJ010000000
VERSION     DASRTJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231157"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFF70_01375"
     CDS             <1..779
                     /locus_tag="VFF70_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43373.1"
                     /translation="IAAFVQFQRKGGLSNDEFTVNLLRVKAADLQPRAFGGYAGALGA
                     RLTHMLWYVHELRDYPASEYWWTSTDRSGLEVNLIDAVNQIEKYGLAWIEDPNAPRPW
                     EMPEYSAAQFVEVLNQVVTPDLIRLGFRSELRRLNGNFPYPYFVKPIDDRQYGIIEFQ
                     QVYSVDPKQFDFDVRLQRKATSDPLDFRGEQRDATNISLGLLVWQINASSFPEMQAGL
                     APLWWKYATRAELIDRLGDVLAKVKQVAIPWFENIELKRQ"
     gene            820..1518
                     /gene="ccsA"
                     /locus_tag="VFF70_01380"
     CDS             820..1518
                     /gene="ccsA"
                     /locus_tag="VFF70_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZY43374.1"
                     /translation="MTHTKDRLTVPLIAITAVLIGVAIYFTFGYAPTADVLSGSDVQR
                     IFYFHVGIAWIAGLALFVTFVCSIIYLWRGENRKYDIIASASVEIGVVFGVMVLLSGM
                     LWARPVWNTWWTWDPRLTTTAITEFLYVAYLMLRGAVDDPQRRARFSAVYGVIAFASV
                     PITYFSTKWFNTIHPDLFDGKSLSPLTAGMKPAMFISLAAFTLVYIVLVRMRAKVETA
                     TDEVAMLKQDLVNN"
     gene            1631..1789
                     /locus_tag="VFF70_01385"
     CDS             1631..1789
                     /locus_tag="VFF70_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZY43375.1"
                     /translation="MQNIVSYLIPAYVIFWLVTFLYVFSIRSRQKNLEKDIEVLKLLA
                     EKKKDASK"
     gene            1971..2510
                     /locus_tag="VFF70_01390"
                     /pseudo
     CDS             1971..2510
                     /locus_tag="VFF70_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
     gene            2589..2729
                     /locus_tag="VFF70_01395"
                     /pseudo
     CDS             2589..2729
                     /locus_tag="VFF70_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
     gene            2749..3348
                     /locus_tag="VFF70_01400"
     CDS             2749..3348
                     /locus_tag="VFF70_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZY43376.1"
                     /translation="MIDINRRRRLNFLGLFASILAIMIGALLLIRSSIQAQTVVAVST
                     KSSTGLIGPAGKTVQAGDMAPDFELATLDERTIKLSDLRGQPVLVNFWATWCGPCKEE
                     MPLIVAASAAHTAEGLRVLAIDTTAFDDVQDVKKFVANYQMTFDVLLDMDDSVGTGWN
                     TLGLPSSYFIDRMGKVVSVRIGQMSAQELSDSLKMILSD"
     gene            3367..3645
                     /locus_tag="VFF70_01405"
     CDS             3367..3645
                     /locus_tag="VFF70_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43377.1"
                     /translation="MKKFVALLLLTAVWLSACGSSAPVAPDASPTVVDQPLTATLPPA
                     SLSQSTANPTANAVPTAAPVSRKLHATNPATVKLASGKPQLVEFFAFW"
     gene            3661..3888
                     /locus_tag="VFF70_01410"
     CDS             3661..3888
                     /locus_tag="VFF70_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43378.1"
                     /translation="MAPIVHGLEAQYVDRINFAYLDIDDPQTDHFKQALNYRVQPHIF
                     LLDAEGHVVQAWLGRVDEAEFVSAFQKVLSN"
     gene            4145..5143
                     /locus_tag="VFF70_01415"
     CDS             4145..5143
                     /locus_tag="VFF70_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZY43379.1"
                     /translation="MRTLKLHQSAPIETQPLVLATSDMPRPSYRSMLLRVRCCGVCHT
                     DLHIVEGDLAPKKLPITPGHQVVAIVEEIGSEVTHYNIGDRVGVPWLNSICGVCEYCR
                     RGEENLCDFARFTGWDVDGGYADYLLADEDIVVPIPAAFSDIDAAPLLCAGIVGYRSI
                     RLADLHPGERLGIFGFGASGHIAIQIARAWNCPVDVFTRSEEHQALARSLGAEWVGRT
                     EDDSPQQLDRAIIFAPSGALIPIALKHLRKGGTLAINAIHTSPIPQMDYQLIYGERTI
                     RTVANATRRDAQEFMSIAAQIPIKSEVQTFPLADANEVLRLLKHSKIKGAAVLVNW"
     gene            5217..5921
                     /locus_tag="VFF70_01420"
     CDS             5217..5921
                     /locus_tag="VFF70_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HZY43380.1"
                     /translation="MSGTLALVGAGEFLDAMNTVDRALLDRSGGLRVAVLPTASAPDG
                     PGVPERWAKLGIEHFNALGAQTDGVMALDRESCNDQSIADRVSAANLVYFSGGKPDYL
                     YETLVDTSVWAAVKSVYDRGGVIAGCSAGAMIMGAYVPSFKMQLGLPHIVSWQPAFGL
                     VKNSIVVPHYNEFPEFVSRLMFRAPKGTTMIGMDGNTALVGSNNSWLVMGQLRVTLRI
                     EGKTMRYLNGQSVQLN"
     gene            5947..6330
                     /locus_tag="VFF70_01425"
     CDS             5947..6330
                     /locus_tag="VFF70_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43381.1"
                     /translation="MDITALHGLIARAMFTFALVAGGFGLFEYFRKHEIGPSYWGIIM
                     VGNLMAVGQGLIGMWLALGPDRQPVREWVHILYGIVAVLWIPIFNFANGYFNKQEDKL
                     KETLILAIISFFECGVALRAIATSV"
     gene            6427..7593
                     /locus_tag="VFF70_01430"
     CDS             6427..7593
                     /locus_tag="VFF70_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43382.1"
                     /translation="MSEEGNKVLVVDDAVQITQTLTDVLGISGYTVRAAPSGERALQI
                     MESADFDLVITDLKMSGMSGMDLAQFVFKQWPGVPVIILSGYGDLDNVISALRSGITD
                     FIKKPFSADEVISVVDRAIGKNRARASSISAQIDNGERPPRLYNFSARELEQIDAALT
                     RLRSQSTAETAMLIEEAGYTIASKGMVSEADRDALTTTVINIRSNASALASMLGEQQD
                     FSMTFMEGQRVSVYTTAIGRGLYLVIIASKGSKQGVVWLYAKEAATEIDRIAQRMVES
                     ERSTGSAALNVVQPTKKKKKTAPLNPPEMKDMPSTVSTITFKKDKPDPVVAADGSPTL
                     SLEEAERLGLINFGDLAVTDETSSEPPAENTSTESVSFEDAIKRGLLDFGDENN"
     gene            7655..8437
                     /gene="pgeF"
                     /locus_tag="VFF70_01435"
     CDS             7655..8437
                     /gene="pgeF"
                     /locus_tag="VFF70_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZY43383.1"
                     /translation="MKQQHHHNLVYYQFDGLAQYSNVYHGLFTRLGGVSRVPFESLNL
                     SRSVGDEAEAVLENNRRMLAIFDAMPHQSVTAWLVHGKTVAVVDRKDAGGYRAATDAI
                     ITNDRSITLTMRFADCAPIVFYDPIHHALGIAHAGWRGIAANIVHEAVSALQSAFQSK
                     PQDLWAGIGPCISVDRYRVGPEVTDAVRAACPPDAPIEQSQPDGSIHLDLNAAIESQL
                     RASGVNQIEVSNLCTATNTHEWFSHRAENGRTGRFGVVIGLH"
     gene            8509..9225
                     /locus_tag="VFF70_01440"
     CDS             8509..9225
                     /locus_tag="VFF70_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZY43384.1"
                     /translation="MNLEANLQRVQDRIAAAALRTGRNPASITLIAVSKTQPVEVLIE
                     AYHLGLRHFGENRVEEAITKLPAFKRAVNDSSVAFHMIGHVQSRKADEIATLFDRVHS
                     IDSVKLAQRLDRIAAVSSKILPILLEVNVSGEVSKYGFEGQAREELLSAAKTIGQLPN
                     LKLDGFMTMAPIASMPDQARSIFRTLRELRGEIASNLIMDLPHLSMGMTDDFEVSIEE
                     GATLVRVGRAIFGERMKAEG"
     gene            9228..9569
                     /locus_tag="VFF70_01445"
     CDS             9228..9569
                     /locus_tag="VFF70_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZY43385.1"
                     /translation="MKKTLSSAKSAVPVSVQTGIAAKEDHSVTFKVKVLPRSSSNSIE
                     GMENGEWKVKLTAPPVEGAANTALIHYLSDVLDVPRRFVTIIGGQSSRSKLVRVDGLT
                     EEQVKQKLGVE"
     gene            complement(9917..>10502)
                     /locus_tag="VFF70_01450"
     CDS             complement(9917..>10502)
                     /locus_tag="VFF70_01450"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZY43386.1"
                     /translation="TIINFGMGSANAATIMDLLSAIEPKACLLLGKCGGLKKKNKLGD
                     LILPIAAIRGEGASNDYFPPEVPALPAFNLQKAISTTIRNTNRDYWTGTVYTTNRRVW
                     EHDDDFKKYLRRIRCMAIDMETATIFITGFYNQIPTGALLLVSDQPMIPDGIKTAASD
                     LAVTQKFAELHLRIGVDSLRELINHGLTVKHLRF"
BASE COUNT         2405 a         2597 c         2962 g         2538 t
ORIGIN      
        1 agatcgccgc gttcgtgcag tttcaaagaa agggcggctt atcgaacgat gagttcaccg
       61 tgaacctctt gcgtgtaaaa gccgccgacc ttcagccgcg cgcatttggc ggttacgcgg
      121 gcgcgttggg cgcaaggctg acgcatatgc tgtggtatgt gcatgaactc cgagactatc
      181 ctgcgtccga gtattggtgg acatccactg atcgatcggg tcttgaagta aatctgatcg
      241 atgcagtcaa tcaaattgaa aagtatggcc tcgcctggat tgaagatccc aacgcacctc
      301 gaccgtggga gatgccggaa tactcggcgg ctcaatttgt cgaagtgttg aatcaagtgg
      361 tcacgcccga tctgattcgc ttgggattcc gatcggaact gcgccggctc aacggaaatt
      421 ttccgtatcc ctatttcgtg aagccgatcg atgatcgaca gtatggcatt atcgaatttc
      481 aacaagtcta cagtgtcgat cccaagcagt tcgatttcga tgtgcggctg cagcgcaaag
      541 cgacgtccga tccattagac tttcgcggtg aacagcgtga tgctacaaat atatcgctgg
      601 gtttgttagt gtggcagatc aacgcatcaa gttttccgga gatgcaagca ggattagcgc
      661 cgttatggtg gaagtatgcc acgcgggcgg agttgatcga tcggctgggc gatgtactgg
      721 ctaaagtgaa acaggttgca attccgtggt tcgagaatat cgaactgaag cgtcagtgat
      781 aaaatagtct gaacagatag gcagtgagga gatcaattaa tgacacatac caaagatcga
      841 ttgaccgtac cgctgattgc gatcacggcg gtgctaatcg gcgtggcaat ctactttacc
      901 tttggctacg caccgaccgc cgacgtgtta tcgggcagtg atgtgcagcg cattttttat
      961 ttccacgtcg gcatcgcgtg gatcgccggg ctggcgctgt tcgtcacgtt cgtatgcagc
     1021 attatctatt tgtggcgtgg cgagaatcgc aagtacgaca ttatcgcgtc agcctcggtt
     1081 gagatcggcg ttgtcttcgg tgtgatggtg ctgctgtccg gcatgttgtg ggcgcggccg
     1141 gtgtggaaca cgtggtggac atgggacccg cgcttgacga cgacggcgat caccgaattt
     1201 ttgtatgtcg cttatctcat gctgcgcggc gcggtcgatg atccgcagcg acgcgcgcgc
     1261 ttcagcgccg tgtatggcgt catcgcgttt gccagtgtgc cgatcacata cttctccacg
     1321 aagtggttca acaccattca ccccgatctg ttcgacggca agagtctgtc gcccttgacc
     1381 gccgggatga agccggcgat gttcatttcg ctggccgcgt tcacgttggt ctatattgta
     1441 ctggtacgga tgcgcgctaa agtcgagacg gccacggacg aagtggcgat gttgaaacaa
     1501 gatctcgtga acaattgaca aattaacaat gaacaatgca tagtgcatca tgcctaaagc
     1561 gtagtggtcg gcatttattt atgcagtgtg cattaagaat tatgaagtgg aggcaatgat
     1621 gagtcctgaa atgcaaaata tcgtttcgta tttgatcccg gcatatgtaa tcttctggct
     1681 ggtgacgttc ctgtatgtgt tcagcattcg atcgcgccag aagaatctgg agaaagacat
     1741 cgaagtgttg aagctgctgg ccgagaagaa gaaagacgca agcaagtaac cacggacact
     1801 tgcaccgcac tgcgtgtgtt gcagtgcagg tgtgacacgg attacactga tgagaagaaa
     1861 atccgtgtga atcctgttaa tccgtgtcca ctagccttgt aagttccgtg taatgacttg
     1921 ctggtatagt tacgataaga aatttcggtg aaggtatgac ccagcaaata atgaatcccg
     1981 tcccgatcgg ttttaccgcg cgctttcaca ctttcgtgca tgcgctgttg ttcatgctcg
     2041 gtctgtcgat cgtgatcgtg ttcggcttcg gcggcgcggc gacgttcatc ggtggcgtgt
     2101 tgaatgacta tgtggtgcgg gatgtgatga cgcgcattgg cggcgtcgcc gtgatcatct
     2161 tcggcctgca cacactcggt atcctgcgca tttcattttt gaattacgac acgcgcaaac
     2221 aatatcaagg cggagccagc tatggctcat cggtgatgat gggcgtgttc ttcgccgccg
     2281 gttggtcacc gtgcatcggc ccgaccttcg gcgcgatcat caccatgggc ttcacccaaa
     2341 ctcaagtcgg caccgcgatg atgctcgccg ccgcttatgc gatcgggctg ggtctgccgt
     2401 ttgttttgct cggcctcgtc gtcgatcgtg ccaccggttt gctgcgccgc ttaaaaaaat
     2461 atcttcgggt atttgagatc atcacgggcg tcttcctatt aatcttgggc gcgctgctgc
     2521 tcagcggcaa gatgaccgag atcgcggcgt tcgccgcgcg cggcggcttg ttgctcgata
     2581 cgagttcgat tgaaacggcc ggcgcgaatc ccaccatccc gatcgccgtt gtggctggat
     2641 tgctctcttt cctctcgccg tgcgtgctgc cgcttgtgcc cgcttacctc ggctacttgg
     2701 gtggacgcgc cgtgacgcag gtcgctaccg aagcgcattg aaactagcat gatcgatatt
     2761 aatcggcgcc gccgattgaa cttcctcggc ttatttgctt ctatactggc gattatgatt
     2821 ggcgcattgc tgctaatccg atcgtcgatc caagcccaga cggtcgttgc ggtgtcgact
     2881 aaatcatcga ccggtttaat tggcccggcg ggtaaaactg ttcaggccgg tgacatggca
     2941 cccgatttcg aattggctac gctcgatgag cgcacgatca aattatcgga tctcagaggg
     3001 caaccggtgc tcgttaactt ctgggcgact tggtgcgggc cgtgcaaaga agaaatgcct
     3061 ttgattgtcg ctgcttctgc ggcacacaca gcggaaggtc tgcgcgtgtt ggcgatcgat
     3121 acgacggcct ttgacgatgt gcaagatgtg aaaaaatttg tagcaaacta tcagatgacc
     3181 tttgacgtac tgttggatat ggatgattcg gtcggcactg gctggaacac acttggtctg
     3241 cccagttcgt acttcattga tcgcatgggt aaggtcgtct cggtgcgtat tggtcaaatg
     3301 tctgcgcagg aattgagtga tagtttgaag atgattttat cggactaagg agacagataa
     3361 ttagagatga aaaaattcgt tgccctctta ctcttgaccg ctgtctggct gagcgcgtgc
     3421 ggttcaagtg caccggttgc tcccgatgca agtccaacag tagttgatca gccgctgacc
     3481 gcaacacttc cacccgccag tctatcgcag tcgacagcta atccaacggc gaatgctgtg
     3541 ccgacggccg cgcccgtcag ccgcaagctg cacgccacta atccggccac tgtaaaattg
     3601 gcttccggta aaccacagtt ggttgaattc ttcgccttct ggtgaattac ttgccagcaa
     3661 atggcgccca tcgtgcatgg gctggaagca cagtacgtcg atcgcatcaa ctttgcttat
     3721 ctcgacattg atgatcctca aacggatcat tttaagcaag ccttgaacta tcgcgttcag
     3781 ccgcacatct ttctgctgga cgccgaaggc catgttgtgc aagcttggct cggtcgagtt
     3841 gacgaagcgg aatttgtcag cgcgtttcag aaagtattga gcaattaaat ttggcagtgt
     3901 tatttcgaat cgcgcagcag tgagaaatct ctagttccat gccttcgtta accgtgagga
     3961 ttagagattc ctcgctgcgc tcggaaggac aggacctgtt ttcgaggcgc agacttctgg
     4021 ccctgcgcct ccttttgttt atgttcgatg aatgcgacta taatcagcgg gctggcatag
     4081 ggatgccatt gcaacaatcc attccccgta gttccggcat ccttaggccg gtacatccat
     4141 tcatatgcga accctgaaac ttcatcaatc cgcacccatc gaaacacaac ctcttgtgct
     4201 tgcgacgtcc gatatgccac gaccctcata tcgatcgatg ttgctccgcg ttcgatgctg
     4261 cggcgtctgc catactgatt tgcacatcgt cgaaggcgat cttgcgccca agaaattgcc
     4321 gatcacgccc ggtcatcaag ttgtagcgat cgtcgaagag attggcagcg aggtcacgca
     4381 ctacaatatt ggcgatcgcg tcggtgtgcc gtggctgaat tcgatctgcg gtgtatgcga
     4441 atactgtcgt cgcggcgaag agaacttgtg tgactttgct cgcttcactg gctgggatgt
     4501 tgatggtggt tacgccgatt atttgttagc cgacgaagat attgtcgtac cgattccagc
     4561 tgcgttcagc gacatcgatg ccgctccgtt gttatgcgcc ggcattgtcg gctatcgatc
     4621 gatcagatta gccgatttgc atcccggcga gcgtttggga atctttggct tcggcgcgag
     4681 cggacacatc gcgattcaaa ttgcccgtgc atggaactgc ccggtcgatg tgtttacgcg
     4741 cagcgaggaa catcaagcgt tggcgagatc gttaggcgcg gagtgggtcg gtcgaaccga
     4801 agatgattcg ccgcaacaac tcgatcgagc gatcatcttt gcgccatcgg gcgcgctgat
     4861 accaatcgcg ttaaagcatt tgcgcaaggg cggcacactc gccatcaatg cgattcacac
     4921 ctcgccgatt ccgcaaatgg attatcaatt gatctacggt gaacgcacca tacgcactgt
     4981 cgcgaatgcc acgcgccgcg atgcgcagga attcatgtcg atcgccgcgc agattccgat
     5041 caaatcggaa gtacagacat ttccactcgc agacgcgaat gaagttttgc gattgttgaa
     5101 gcatagtaaa ataaaaggcg cggcagtgtt ggtaaattgg tagtggcaag cagcactact
     5161 tgctcttcat taacaaacgc gcgataattg gcaatagccc atcgggggac gagacaatga
     5221 gtggtacact ggcgttagtc ggcgcagggg aatttctcga tgcgatgaat acggtcgatc
     5281 gggcgttgct ggatcgatcg ggcggcctga gagtcgccgt attgcccacc gcctctgcgc
     5341 ccgatgggcc gggtgtgccc gagcgctggg ccaagctggg catcgaacat tttaatgcac
     5401 tcggtgcaca gaccgatggg gtgatggcgc tcgatcgaga atcctgcaac gatcaatcta
     5461 tcgccgatcg ggttagcgcg gcgaacttgg tttatttctc gggcggcaaa cccgattatc
     5521 tgtatgagac attggtcgat acgtccgtgt gggctgccgt aaagtcagtg tacgatcgcg
     5581 gcggcgtgat cgccggttgc agcgcaggcg cgatgatcat gggtgcgtac gtgccaagct
     5641 tcaaaatgca attaggtctg ccgcacatcg tgagctggca gcccgcgttt ggcttggtga
     5701 aaaactcgat tgtcgtgccg cactacaacg aatttcctga attcgtgtcg cgcttgatgt
     5761 tccgcgcgcc caaaggaacg acgatgatcg gcatggatgg caacaccgcg ctggttggat
     5821 cgaacaatag ctggctggtg atgggtcaat tgcgcgtgac gctgcggatc gaagggaaaa
     5881 cgatgcgtta tttaaacgga caatcagtcc agttaaactg atcttatttt ataaacggag
     5941 tcaagcgtgg atattacggc actacatgga ttgatcgccc gggcgatgtt cacctttgct
     6001 ttagtggcgg gcgggttcgg cctgtttgaa tattttcgca aacatgaaat cggccccagt
     6061 tattggggca tcatcatggt cgggaatctg atggccgtgg ggcaagggct gatcggtatg
     6121 tggctggcgc tggggcccga tcgacagccg gtgcgcgagt gggttcatat tttgtatggc
     6181 atcgtcgccg ttttatggat tccgattttc aattttgcca acggctattt caacaagcag
     6241 gaagataaac tcaaagaaac gttgatcctc gccatcatat catttttcga atgcggcgta
     6301 gcgctgcggg cgattgcgac cagcgtataa ttcattgcgt gcggctgaag agcggcctga
     6361 cttaaatttt ggtaggcgtg ctctcttaca tacggtgggc tcaggtaacc gatcgaggat
     6421 gtcaggatga gtgaagaagg taacaaagtt ttagtcgttg atgacgctgt gcagattacc
     6481 caaacactga cggatgtgct ggggatcagc ggctacacgg tgcgagccgc gccaagcggt
     6541 gagcgcgcgc tgcagatcat ggaatcggcc gatttcgatc tggtcatcac cgatctcaag
     6601 atgtctggca tgagcggcat ggacttggct cagttcgttt tcaagcagtg gccaggtgtg
     6661 ccggtgatca tcctcagcgg ttatggcgat ttagataatg tgattagtgc gctacgatcc
     6721 ggcatcactg acttcatcaa gaaaccattc tcggctgacg aagtgatctc cgtggtcgat
     6781 cgggcgatcg gcaagaatcg cgcgcgggcg tcatcgatct ctgcacagat cgacaatggc
     6841 gaacggccgc cgcgcctata caacttttcg gcgcgtgagc tggaacagat cgatgcagcc
     6901 ttaacccgtt tgcgctctca gtcgacggct gaaacggcga tgctgatcga agaagcaggt
     6961 tacaccatcg cctcgaaagg gatggtcagt gaagccgatc gagatgcgct gacgaccacc
     7021 gtcatcaaca tccgatcgaa tgcttcggcg ttagcgagta tgttaggcga gcagcaagat
     7081 ttcagcatga cgttcatgga aggccaacga gtcagcgttt atacaacggc gattggtcgt
     7141 ggcttgtatc tggtgattat cgcgtcgaaa ggctccaaac aaggtgtggt gtggttgtat
     7201 gctaaggagg ctgcaactga gatcgatcgg attgcgcagc gcatggtaga gtcagagcgt
     7261 tcaaccggct cagccgcact aaatgtcgtt caaccgacga aaaagaaaaa gaagacggct
     7321 ccgctcaatc cgccggagat gaaggacatg ccctcgacag tttcgaccat tacgtttaag
     7381 aaagataaac ccgatccggt cgtagcagca gatggcagtc ccacgttgag tcttgaagaa
     7441 gccgagcggc tgggtctgat caacttcggc gatctggccg taaccgatga gacatccagc
     7501 gaaccaccag ctgaaaatac gagcacagaa tcagttagtt ttgaagatgc aatcaagcgg
     7561 ggtttgttgg attttggcga tgaaaacaat taactgcgaa gacctcaagg cgcaaggaaa
     7621 ataattcctc cccaccccaa aggcataagt tagattgaag cagcagcatc atcacaacct
     7681 cgtctattat caattcgacg ggctcgctca atattccaac gtctatcatg gcctctttac
     7741 gcggctcggc ggcgtgagcc gcgtaccatt tgaatcgcta aacctctctc gatcggttgg
     7801 cgatgaagcc gaggccgtgc ttgaaaataa tcggcgcatg ctggcgatct tcgatgccat
     7861 gccccatcaa tctgtcacgg cctggctggt gcatggcaaa accgttgcag tggtggatcg
     7921 aaaagatgca ggcggctatc gagctgcgac cgacgcgatc atcaccaatg atcgatcgat
     7981 tactctgacc atgcgttttg ccgactgtgc gccgatcgtg ttttatgatc ccattcatca
     8041 cgcgttaggc atcgcgcatg cgggctggcg tggaatagcg gccaacattg tgcatgaagc
     8101 ggtgtcagcg ctgcaatcag cttttcagag taagccgcaa gatttgtggg ctgggattgg
     8161 gccatgcatc agtgtcgatc gatatcgcgt tggaccggaa gtgaccgatg ctgtcagagc
     8221 cgcgtgtccg ccagatgctc cgatcgaaca atctcaaccc gatggatcga tacacctcga
     8281 tctgaatgcg gctatcgagt ctcaactgcg cgcgagcggc gtgaatcaaa ttgaagtatc
     8341 gaatttgtgc acggcgacaa atacgcacga atggttttca catcgagccg agaatggtag
     8401 gacgggcagg tttggcgtgg tgattggctt acattgaact aagccttcac atcattatac
     8461 ctatttctta ttcgtgcaga ttcgtggtga ttcgtagatt attttctgat gaacctcgag
     8521 gctaatctcc aacgagtaca agatcgcatc gccgccgcgg cgcttcgcac agggcgtaat
     8581 cccgcatcga tcaccttaat cgccgtcagc aaaacgcagc cggtcgaggt gttgatcgaa
     8641 gcctatcatc taggcctgcg gcacttcggc gagaatcgcg tcgaagaagc gatcactaag
     8701 ctgccagctt tcaagcgtgc cgtcaatgat tcatcagttg cttttcacat gatcggtcat
     8761 gtacaaagcc gaaaggccga cgaaattgcc acactattcg atcgtgttca ttcaatcgat
     8821 agcgtgaagt tggcgcagcg cttggatcga attgcagcgg tgagtagcaa gatactgcca
     8881 atcttgttgg aagtgaatgt gtccggcgaa gtttccaaat acggcttcga gggtcaagcg
     8941 cgtgaagaat tgttgagcgc cgcaaaaacg atcgggcagc tgccgaactt gaaattggat
     9001 ggtttcatga ccatggcacc gatcgcgagc atgcctgatc aagcccgatc gatcttccgc
     9061 actctgcggg aactgcgcgg tgaaatcgca tcgaacttga taatggatct gccacatctt
     9121 tcgatgggca tgacggatga ttttgaagtc tcaatcgaag aaggcgcgac gctggttaga
     9181 gttggccgag cgatctttgg agaaagaatg aaagcagaag gttaaaaatg aaaaaaactc
     9241 ttagcagtgc gaagagcgcc gtgccagtca gtgtgcaaac cggaatagcg gcgaaagaag
     9301 atcactcagt gaccttcaaa gtaaaagtgt tgccacgctc aagcagcaac tcgatcgaag
     9361 gcatggagaa cggcgagtgg aaagtgaaat tgactgcgcc gccggtcgag ggtgcggcga
     9421 acaccgcgct gattcattat ctatcggacg tgctggatgt gccacgcagg tttgtgacga
     9481 tcatcggcgg tcagagttcg cgcagcaaac tcgttcgcgt cgatggctta actgaagaac
     9541 aagtcaaaca gaagttgggc gttgaataaa taactgatgt tgcgcagttg cggccgcagt
     9601 tgcactgctg ccgccgcagt gcaactgcgg tgtgaactta agaagctgag tgtggaataa
     9661 ttaaaacttc ggaagtcact cagacttccg aagttttatt gtatgttaca tcatcgcgag
     9721 ctgcgcaaag tgcggcatgg caatctctac cactgtaaat taactcgaaa ctagcttact
     9781 caaagcccgt gtccgcgcaa gcgtttcacc atttcgattc gaccgcgagg acgtggtcag
     9841 agagcggaac ctagcaggtg tgacggcaca gcggatgaat gatcacatcc aattttcttg
     9901 aagcaaactc cttgctttag aaacgcagat gcttcaccgt caacccgtga ttgatcaact
     9961 ccctgagtga atcgacgccg atacgcagat gcagctcggc aaatttttga gtgaccgcta
    10021 gatcgctggc ggcggtttta atcccgtcgg ggatcatcgg ctgatcggac accagcaata
    10081 gcgcgccggt gggaatctga ttgtagaagc cggtgatgaa gatcgtcgcg gtttccatat
    10141 caatcgccat gcagcggatg cggcgtagat attttttgaa atcgtcgtcg tgttcccaca
    10201 cccggcgatt agtcgtgtag accgtgccgg tccagtaatc gcgattggtg ttgcggatgg
    10261 tcgtcgaaat cgctttctgc aaattaaaag cgggcagggc ggggacttcc ggcggaaagt
    10321 aatcattgga tgcgccttcg ccgcggatcg ccgcgatggg tagaatcaaa tcgcccagtt
    10381 tatttttctt cttcaggccg ccgcattttc ccaacaacaa acacgctttt ggttcgatcg
    10441 cactgagcag atccatgatc gtcgcggcat tggcgctgcc catcccgaag ttgatgatgg
    10501 tg
//
LOCUS       DASRTJ010000075         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_306908,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000075 DASRTJ010000000
VERSION     DASRTJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306908"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFF70_01455"
     CDS             <1..585
                     /locus_tag="VFF70_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY43387.1"
                     /translation="VLAKVPFSKKEFMREASVKKLWGEKGYTPIEQTAARPTLELNGI
                     WGGWTGEGSKTVLPSKAYAKISCRLVADQDFVEIKDLLIKHLKKIAPKEVGVNIKWLH
                     GGQGALVNLDSSYMRAAAKALKEVFGHEPVYLREGGSIPVVAAFQEFMGIDSIMMGFG
                     LPDDNLHSPNERFFLPNFYNGIQATIRYLEEVGK"
     gene            complement(723..1010)
                     /locus_tag="VFF70_01460"
     CDS             complement(723..1010)
                     /locus_tag="VFF70_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HZY43388.1"
                     /translation="MLDVYCADQKLMIEIDGDSHFGPDQIEYDQARTKWLNEEGYRVI
                     RFTNREVVNQFEAVLEKTLEECSRPSPKPSPRYALRASVRPAGSTARVPHV"
     gene            complement(1026..2135)
                     /locus_tag="VFF70_01465"
     CDS             complement(1026..2135)
                     /locus_tag="VFF70_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43389.1"
                     /translation="MTTILVIDDDASIRNNLLDMLETEGFKAAGAENGLKGVHLAQAS
                     PPDLVICDIMMPELDGYGVLQALRTDGRTAMLPFIFLTAKAERADLRFGMEQGADDYL
                     TKPFTRNEVMSAITARLQRAQKVREQTHAKYNQLKQQISTLLAHELRTPLIPIMGYTD
                     LALDNISALSPEEMLGFLGRIEQGSVRLTRLVENLLMLVQIDTGQILGDFAATVKVCR
                     NLGEVITLAVESYRLSALIQGVSLGVKVDADLPAVRLSQPLFATTFGHLIDNGIKFSK
                     NRLGAVTISVHRAGDGVDIALVDHGVGIPADEIPHLFERLQQIDRATHEQQGSGTGLA
                     IVQELIKLHGGTIRVESVLGSGSTFTIHLPEYHET"
     gene            complement(2161..>3405)
                     /locus_tag="VFF70_01470"
     CDS             complement(2161..>3405)
                     /locus_tag="VFF70_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZY43390.1"
                     /translation="ITDRKQREREMAALVSVAAALRAAPTRGDMLPVIVDKAMSLIKA
                     DGGALVMHSPATAEEIFVLSRGNWSDLTGTRLPAGQGISGHVITTGRPYVAGDVRTDP
                     YLDIAAEQLGGAYAAAGVPLLAEQQTIGALLIGRQTGFTPADVRLLTAIGDITANALY
                     RAILIETLEHRVEERTQELTAANQQLIELGQLKDQFISMVSHEYRTPLTTILSSAEML
                     DLYQAKWSVEKKHEHLQQIQASVKRMTELLDQILIIGKAEAGKLAFNPAPVDLLKICR
                     DVAEELQFSTGREDTLTFTHRGECADARLDVNLVRHILSNLLSNAVKYSPKGSSVEFE
                     VTCKTDWAIFQITDHGIGIPPEDQARMFETFHRAKNVGNIQGTGLGLAIVKHAVDRHG
                     GTIEVVTEVNKGTTCTVRLPIG"
BASE COUNT          742 a          919 c          969 g          775 t
ORIGIN      
        1 gtgctggcga aagtgccgtt cagtaaaaaa gaattcatgc gcgaggccag cgtgaaaaaa
       61 ttgtggggcg aaaaagggta cacgcccatc gaacaaaccg ccgctcgacc gacgttggaa
      121 ctcaacggca tttggggtgg ctggacgggc gaaggcagta agaccgttct cccatcgaaa
      181 gcgtatgcga agattagctg ccgcttagtg gcggatcaag atttcgttga gatcaaggat
      241 ttgttgatca agcatctgaa aaagatcgcg ccgaaggaag tcggcgtcaa tatcaaatgg
      301 ctgcacggcg ggcagggtgc attggtgaat ctcgattcgt cttatatgcg cgccgctgcg
      361 aaagcattga aggaagtttt cggtcatgag cctgtctatc ttcgcgaagg cggatcgatc
      421 ccggtggtcg ccgcgttcca ggaattcatg ggcatcgatt cgatcatgat gggcttcggt
      481 ttgcccgacg ataatttgca ttcgcccaac gagcgtttct tcctgccgaa tttctacaac
      541 ggcattcagg cgacgattcg gtatttggag gaagtgggga agtagttgga tagttgggta
      601 gtttggcagt cagaaaagca aagtccgatc ttcgtaaaga ggatcggact ttttttgatc
      661 caccctcatc cccaaccctt ctccctaaaa gcggagaagg gggcaagaat tccctctcca
      721 tttcacacgt gcggtacccg tgcggtagag cccgcagggc gtaccgaagc acgcagtgcg
      781 taccggggag agggcttggg tgagggtcga ctgcactctt ccaatgtctt ctcaagcacg
      841 gcctcaaatt gattcaccac ttcgcgattc gtaaagcgaa ttacgcgata accttcttca
      901 ttcaaccatt tcgtgcgagc ttgatcgtat tcaatttgat ctggcccaaa atgcgagtcg
      961 ccgtcgattt cgatcatcag tttttgatcg gcgcagtaga catcaagcat aacgcgccga
     1021 ccgatctagg tctcgtgata ctccggcaga tggattgtaa acgtgctgcc cgaacctaac
     1081 acgctctcga cacggatcgt gccgccatgc aatttgatca attcttgcac aatcgccagg
     1141 cccgtgccgc tgccttgctg ctcgtgagtc gcgcgatcga tttgttgcaa gcgttcaaac
     1201 agatgtggaa tctcatcggc aggaatcccc acgccatgat cgaccagagc aatgtcaacg
     1261 ccatcgccgg ctcgatggac tgaaatggtg accgcgccca gtcggttctt ggaaaacttg
     1321 atgccattgt caatcaagtg gccgaaggtg gtcgcaaaca gcggttggct caaacgcacc
     1381 gcgggcaaat ccgcatcgac tttgacgccc agactcacgc cctgtatcag cgctgacaag
     1441 cgataagact cgacggccag cgtaatgacc tcaccgaggt tgcggcagac cttgactgtt
     1501 gcggcgaagt ctccgaggat ctgcccggtg tcgatttgga ccaacatcaa aagattttcc
     1561 acgaggcgag tcaggcgcac tgagccctgt tcaatcctcc ccaagaaacc gagcatttcc
     1621 tcgggcgaga gcgcggagat attatccaaa gcgagatcgg tataacccat aatcgggatc
     1681 agcggggtgc gcagttcatg ggccaacagg gtgctgatct gctgcttgag ttgattgtat
     1741 ttggcgtgag tctgctcgcg aaccttctgc gctcgctgca aacgggccgt gatggcgctc
     1801 atgacctcgt tacgggtgaa gggtttcgtt aagtaatcgt cggcaccctg ctccataccg
     1861 aaccgcagat cggcgcgctc ggctttggcg gtcagaaaga tgaacggcag catggcggtg
     1921 cgtccatctg tccgtaaagc ttgcagcaca ccgtacccat ctagttcggg catcatgata
     1981 tcgcaaataa cgagatcggg gggactcgct tgcgccaagt gcacaccttt caatccgttt
     2041 tctgcaccgg cggctttgaa gccttcggtc tctaacatat cgaggagatt attgcgtatt
     2101 gacgcatcat catcgatgac gagaatcgtg gtcatgggag ctcctttatg agtttacgga
     2161 tcacccgatc ggtaatctca cggtgcaggt ggtgcccttg ttcacttcag tgacaacctc
     2221 aatggtcccg ccgtgtcgat cgaccgcgtg cttgacaatg gctaagccta gcccggtgcc
     2281 ctgaatgttg ccgacgtttt ttgctcgatg aaatgtctca aacatgcgag cctgatcttc
     2341 tggcgggatg ccaataccat gatcagtaat ctggaagatc gcccaatcgg ttttacaagt
     2401 cacctcaaac tcgactgagc taccttttgg cgagtacttc accgcattcg atagcaaatt
     2461 gctcaagata tggcgcacca gattgacatc caaacgggca tcagcgcatt caccgcgatg
     2521 ggtaaaggtc aacgtgtcct cacggcccgt tgagaattgc agctcttccg ccacgtcgcg
     2581 gcaaattttc agcagatcga ccggcgctgg attgaaagcg agtttgccgg cctccgcttt
     2641 gccaataatg agaatttgat ccagcagctc ggtcatgcgc ttgacggagg cttgaatctg
     2701 ctgtaaatgt tcatgttttt tttcaacgga ccatttggcc tgatacaaat ccagcatctc
     2761 ggctgacgat aggatggtcg tcaggggtgt gcggtattcg tggctgacca tggatataaa
     2821 ttgatccttc aactggccga gctcgatcag ctgttggtta gccgcagtca actcctgggt
     2881 gcgttcttcg acgcgatgct ccagcgtttc gatgaggatg gcccgataca gcgcattggc
     2941 ggtgatgtcg ccgatggcgg tcagcaggcg cacatcggcc ggggtgaagc cggtttgccg
     3001 gccgatcaac aacgcgccga tggtctgctg ctcggccagc aatggcacgc cggccgcggc
     3061 atacgcgccg cccagctgct cggcggcgat atccaagtac ggatcggtgc gcacatcgcc
     3121 ggcgacgtag ggccggccgg tggtgatgac gtggccgctg atcccctgac cggccggtag
     3181 gcgcgtgccg gtcaagtctg accagttgcc gcgcgacagg acaaagattt cttccgcggt
     3241 ggctggcgag tgcatcacca gcgcaccgcc atcggccttg atcaggctca tagccttatc
     3301 gacgatgacg ggtagcatgt cgccgcgggt gggcgcggcg cgcagggccg cggctacgct
     3361 gactaacgct gccatttcac gctcgcgctg cttgcggtct gtgat
//
LOCUS       DASRTJ010000076         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_79823,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000076 DASRTJ010000000
VERSION     DASRTJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79823"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFF70_01475"
     CDS             complement(<1..194)
                     /locus_tag="VFF70_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43391.1"
                     /translation="MIDFSHGPSATENHRHNLVVNEKVFARSKFLRLSTIVALTALAF
                     CVLALMLSAAAGRSAVAQPNG"
     gene            complement(200..1627)
                     /gene="purB"
                     /locus_tag="VFF70_01480"
     CDS             complement(200..1627)
                     /gene="purB"
                     /locus_tag="VFF70_01480"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZY43392.1"
                     /translation="MNWSNGPLTIDHSTFQSSFSWRYGSENMRSIWSEEHKRKLWRRI
                     WIALAAAEAQFGLVTDEQLADLKAHADQIDLDRALAIEAEIKHDLMAEVKTFAEQCSI
                     GGGIIHLGATSMDVEDNADALRVRESIDLILTSLSALLKSFAAQIEKWADVPTMGFTH
                     LQPAEPTTIGYRLAQYMQDLLVDYEELIRVRREIKGKGFKGAVGTSASYLELVQDSLA
                     KTLKPSLNLEKLVMRALDLDAFDAATQIYPRKQDWLVLNALAGVAMSLNKFAFDLRVL
                     QSPPIGEWREPFGSKQVGSSAMPFKRNPINAEKIDSLARYLAQLPRVAWDDAAQSLLE
                     RTLDDSANRRIILPEAFLCADEILITAQKLIDGLVIDQPAVNRNFEIYAPFAATEKLL
                     MALAKAGADRQDLHEVIREHSLAAWAEVSAGKPNPLIEALCSDPHITLYLHSDKARSI
                     LDARGYIGDAPRRARKIASKVRALD"
     gene            complement(1727..>2809)
                     /locus_tag="VFF70_01485"
     CDS             complement(1727..>2809)
                     /locus_tag="VFF70_01485"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZY43393.1"
                     /translation="GMVLNLRSLAREVDELNQIGANLNPDRLKIDRAAQLLLPGHIAL
                     DGASESDRGDQAIGTTKRGIGPAYTDKAARVGVRAGLMSDRSTFSAAVRSHVDRSNAR
                     LKHDFAQKPIDAETIVTEYVALADRFAAHLTDVNALVHTALERGEHILAEGAQGTLLD
                     IDHGTYPFVTSSSTIAGGVLTSLGVGPKEVTRVIGVAKAFCSRVGSGPFPTELKDALG
                     DRLRGTGSNPWDEFGSTTGRPRRTGWLDLVALKYAARLNGLTELVLTKLDILSGLDPL
                     HVCMAYSYHDQPITNFPGDLAILDDCTPIYSELPGWDKDISKARNITDLPLPVQKYIH
                     QIETFTGVKVSFVSVGPERSQIFETS"
BASE COUNT          628 a          840 c          725 g          616 t
ORIGIN      
        1 ccgttcggct gagcaacggc cgaccgaccg gctgccgcgc ttaacatcag cgccaaaacg
       61 cagaaagcca gtgcggtcag agcaacgatt gtgcttaagc gcaagaattt cgatcgcgca
      121 aaaacctttt cattcacgac caagttgtgc cggtgattct cggttgcgct gggaccgtgg
      181 ctaaaatcga tcaacacatt taatccaaag ccctgacttt cgaggcgatc ttccgcgccc
      241 gccgcggtgc atcgccgatg tagccgcgcg catccaagat cgagcgggct ttgtccgaat
      301 gaagatagag cgtgatgtgc ggatcgctgc acagtgcctc gattaacgga ttcggcttgc
      361 ccgccgaaac ttcggcccac gcggccagcg agtgttcgcg gatcacttca tgcaaatctt
      421 gtcgatcggc tccggcttta gccagcgcca tcaacaattt ttctgttgcg gcaaaaggcg
      481 cgtatatttc aaaattgcga ttgaccgcgg gctgatcgat aaccagcccg tcgatcaatt
      541 tttgcgccgt aatcaggatt tcatctgcgc acaaaaaggc ttccggcaaa atgatccggc
      601 gattggccga atcatctaac gtgcgttcca ataacgactg cgcggcatca tcccaggcca
      661 cgcgcggcag ttgcgccaga taacgcgcca gtgaatcgat cttctcggcg ttgatcggat
      721 tgcgcttgaa gggcatggcc gacgagccaa cctgctttga gccaaacggt tcgcgccact
      781 cgcctatcgg cggcgattgc agcacgcgca gatcgaacgc gaatttgttc agcgacatcg
      841 ccactccggc taaggcgttc aacaccagcc aatcctgttt gcgcggataa atttgcgtcg
      901 ccgcatcgaa ggcgtccaga tcgagcgcgc gcatgacaag tttttctaaa ttcaatgaag
      961 gctttagcgt tttggctaaa ctatcttgca ccaactccag atacgaagcc gatgtgccga
     1021 ccgcgccttt gaaacccttg cccttgatct cgcgccgcac gcggatcaat tcttcgtaat
     1081 caactaataa atcctgcata tactgggcta aacgataacc gatcgtcgtc ggttcggcgg
     1141 gttgtaaatg tgtgaagccc atcgtcggca catcagccca tttttcaatt tgtgcggcga
     1201 atgatttcag caatgcgctg agtgaagtca aaatcaaatc gatcgattcg cgcacacgca
     1261 gcgcgtcggc gttgtcttcc acgtccatcg acgtggcgcc gaggtgaatg atcccgccgc
     1321 cgatcgagca ctgctcggcg aaggtcttca cttcggccat caaatcatgc ttgatctcgg
     1381 cttcgatcgc caatgcccga tcgagatcga tctgatccgc gtgggctttg agatcggcca
     1441 attgttcgtc ggtcaccagc ccgaactgcg cttcagctgc ggctaacgcg atccagatgc
     1501 gccgccacaa tttgcgcttg tgctcttcgc tccagatcga gcgcatgttc tcagaaccat
     1561 aacgccacga aaaagaggat tggaaagttg aatgatcgat tgtcaatgga ccattactcc
     1621 aattcacacg tcattgcgag aagtgccgta ggcacgacga agcaatctcc tactcgatgc
     1681 tgagattgcc atgccgccta ctgcggctcg caatgacgca catcgtttag ctcgtttcaa
     1741 aaatctgcga tcgctccggc cccaccgaaa caaacgaaac cttcaccccc gtgaaggttt
     1801 cgatctgatg aatatatttt tgaaccggca gtggtaggtc cgtgatattc cgcgctttcg
     1861 aaatatcttt gtcccagccc ggcagttcgg aatagatcgg cgtgcagtcg tcgaggatcg
     1921 ccagatcgcc gggaaaattg gtgatgggtt gatcgtgata agagtaagcc atacacacgt
     1981 gcagcggatc gaggccgctg agaatatcca acttcgtcaa gacgagttcg gtcaagccat
     2041 tcaagcgcgc cgcatatttc aatgcgacca gatcgagcca gcctgttctg cgcggccgcc
     2101 ccgtggtcga tccgaattca tcccacggat tcgatcccgt gccacgcaac cgatcgccca
     2161 gcgcgtcctt caattccgtc gggaacggac ctgatccgac gcgcgaacaa aacgctttcg
     2221 ccacgccgat cacgcgcgtc acctctttcg gtccgacgcc taaacttgtc aacacgccgc
     2281 ccgcgatggt ggacgagctg gtcacaaacg gataagtgcc atgatcaata tccagtaacg
     2341 tgccttgcgc accttccgct aaaatatgtt cgccgcgttc cagcgccgta tgcaccaacg
     2401 cattgacgtc cgttaaatgc gcggcaaacc gatcggccaa cgccacgtac tcagtcacga
     2461 tcgtttctgc atcgatcggt ttctgcgcaa aatcatgttt caggcgtgcg ttcgatcgat
     2521 cgacgtgtga tcgaaccgcc gcgctgaagg tcgatcgatc agacatcaat ccggcgcgca
     2581 cgccgacgcg cgcggccttg tccgtatatg cggggccgat gccgcgcttc gtcgtcccga
     2641 tcgcttgatc accccgatcg gattcactgg cgccgtccag cgcgatgtgg cccggcagca
     2701 agagctgcgc cgcccgatcg atcttcaagc gatccggatt taagttcgcg ccaatctgat
     2761 tcagctcatc cacttcacgc gcgagcgatc gtaaattgag gaccatgcc
//
LOCUS       DASRTJ010000077         6655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_4378,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000077 DASRTJ010000000
VERSION     DASRTJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFF70_01490"
     CDS             <1..1486
                     /locus_tag="VFF70_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY43394.1"
                     /translation="YIEKDAALERRFAPVFVDEPNVDETIEMLHGLRDRYEAHHKVRI
                     SDEALVAAAHLSQRYVTDRRLPDKAIDLIDEAAAKLRVSLYTLPADLKEMKSEIDRVT
                     AEEEAAGLARDYENAAILKSKRLQIETDFNQKREEWQREKNLDEVVDEGDIASVIAQW
                     TGIPVAQMMETESAKLLQMEERLHDRIIGQEEAIAALSDAIRRARSGLKDPSRPIGSF
                     IFLGSSGVGKTELAKALAWFMFDDEDALVRVDMSEYREQHTASRLFGAPPGYVGYEEG
                     GQLTEAVRRRPYQVVLFDEIEKAHPEVWNSLLQILDDGRMTDGQGHVVDFRNTVIIMT
                     SNLGTEFGRKGGALGFTRPGVAPAQIADAKNIEDQLKRTFRPEFLNRIDEIIIFGTLS
                     VEDVEKIVDLQMNDIRSRLRDQGLFIELSEAARQWLAKEGYDPQFGARPLRRALQRHV
                     ESPLSVKMLKGEFKVGDTIMVDANESGLVFSQKEKVSVTQDQPA"
     gene            complement(1559..3271)
                     /locus_tag="VFF70_01495"
     CDS             complement(1559..3271)
                     /locus_tag="VFF70_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43395.1"
                     /translation="MLKLVKSFVVGWFAVVWLGITLSSSSASSAHPAAADRRPINPSE
                     LHRTATITTTIYFPIIAVNYIPPPTPVMVSLRLGLNFVSSAEAPANTTRYQQAADLNP
                     AINRWPFYWYGIEPQPITQPRTFDWSKIDANVIADTQHNLPIDAILLGTPPDLATGGS
                     ALAPRPKIGDGLRVLEKSLSTNGPTTISSVASPPIGLYDGIYANGVITNDVPGKPINP
                     NNRWAYFVNAAVNRYKPGGTIAQAQGWLSTTGITHWEIWNEEDLNFFFSGTITDYARL
                     LKVAYLSAKSADPHAVIVLGGMAHFEKPTWLNDTLNIIATDPLSITYHGYMDAVASHS
                     YAWSWQTFNYLYQDRSQLNARGLNNVRLWLTETGLPVCDDPPYVFCPSTYRGSMHEQA
                     NYLIQTLTFAQWLNAEMAIWFQMVDDCGNDGHYDAFGLIRNPASMPACPQTARDGTTR
                     SSYDTYQVFANRVLGAVPYWRKRPTSNQELIAFKRASAGQRIVVMWARSNVAETVVLS
                     ATSSSAELISPDGSTQAIFSINGVYTITLPAATNYSTPTNDGSAAIGGDPRILIETDP
                     DVTP"
     gene            3413..4210
                     /locus_tag="VFF70_01500"
     CDS             3413..4210
                     /locus_tag="VFF70_01500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY43396.1"
                     /translation="MGLFDGQVAWVTGAGRGIGRAAALALAEQGASIAIVSRTQSELD
                     QTADEIKARGGKVLTSLLDVSNWDMVEWTAKQIESLLGPIDLLINNAGVLEPLGKLWE
                     TAPEEWTRLIDINVSGAYYCMRAVLPGMVKRDRGVVINISSGAATNAAPNWTAYAASK
                     AALDQLTRTLAVDLKGTAVRTYSLHPGITESNMQHTLRNATADQLPPERSQFFIDEKI
                     SGNVLDPSVPAQAIVWLCSALCDLDNGAMIDLRSQPEYVEKITQALR"
     gene            complement(4313..>6655)
                     /locus_tag="VFF70_01505"
     CDS             complement(4313..>6655)
                     /locus_tag="VFF70_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43397.1"
                     /translation="LQGELKGFYLARRLWTVSSVSRLGLIALLIGTWLTTTAFSTRQS
                     STATLIGLVMFTLGWAFALTAAVNSAPIVYLIVSAYLGWYGILIGGALAGSLGFALPT
                     LWMLILGWQVMRRPIRRIDRVCSFLLCFGAAYLTYGAFGLSHLWVPIPLASGVLILAL
                     IYFGALFNPIVRRWAIPSRLDAIKNPRSIFWLTLLIVGSFFALSYTRDAAQSAANANL
                     AMQNLLGAADLFWMWLGGGLFAGSLGLGRWLTREAMQIVTARWLRWFIPIGWLAVAIF
                     GYLATGPLPLSIAVFAYHLGLTDWVSTWSNPDYFAVQAQVCVSLIALAYLIILRLRHT
                     YTDNKLAWANGLWIAAYLSVLGYHSSLMGFAGIDQRSIQSLLAWAALALVGGILWRMA
                     KHGADWSGESSSRLYSLLAMLLIMLSVISIVLSANAVQFIQESTLYSFYGIVYLGIPL
                     ALYTLLTNQSHFVPMRGIKLAALFALGSLSAIVVLGINPLAGIHLLIAPLIWAIVLSS
                     HRLQREDRLNQLVAGGALALGFAVFWLKPLVLPIPFLQIVQDWQAQYSTAALNRSGMQ
                     LGQLWFTAAALIVGVAIGWIMIQRRSQLRWLLIAFCAVAFGLIVPRLPEISKSDATLI
                     ANQPGSIPSIVSTPVPNWIETNLVSTTLKISIPPDWSPNPNGAQGELLNLISPQGDLI
                     FSVALFDGATDVQKLAQAANEIRRGSFADYQIDVGPEQRSSNGKNILWLEWSSAANST
                     RGVDAYTIIPQGILQFSLWSAPNAFEQRRAEFEQIINSLQ"
BASE COUNT         1623 a         1752 c         1922 g         1358 t
ORIGIN      
        1 gtatattgag aaagacgcgg ctttggagcg gcgctttgcg cccgtgtttg tcgatgagcc
       61 gaacgttgat gaaacgatcg agatgctgca cggcttgcgc gatcgatacg aggcgcatca
      121 caaggtgcgc atcagcgatg aagcgctggt cgccgccgcg catttgtcgc agcgctacgt
      181 aaccgatcgg cggctgcccg ataaggcgat cgatctaatc gacgaagcgg cggcgaagct
      241 gcgggtgtcg ttgtataccc tgcccgccga tctcaaagag atgaaatctg aaatcgatcg
      301 ggtgaccgct gaagaagagg cagctggact ggcgcgggat tatgagaacg ccgcgatctt
      361 gaagagcaaa cgcttgcaga tcgaaacaga tttcaatcag aagcgtgaag agtggcagcg
      421 tgaaaagaat ttggatgaag tggtggatga aggcgacatc gcttcggtca tcgcgcaatg
      481 gacgggcatt ccggtggcgc aaatgatgga gacggaatcg gccaagctgc tgcagatgga
      541 agaacggctg cacgatcgga tcatcggcca ggaagaagcg atcgcggcac tgagcgatgc
      601 gatccgccgc gctcgatcgg gcttgaaaga tccatcgcgt ccgatcggct cgttcatctt
      661 cctcggctcg tccggcgtgg gcaagaccga actcgccaaa gcgctggcct ggttcatgtt
      721 cgacgatgaa gatgctttag tgcgcgtgga tatgagtgag tatcgcgagc agcacacggc
      781 ctctcgcttg ttcggagcgc cgcccggcta tgtgggctat gaagaaggcg gccaactgac
      841 tgaagccgtt cgacgccgcc cgtaccaagt ggtgttgttc gacgagattg agaaggcgca
      901 tcccgaagtg tggaactcgc tgctgcaaat tttggatgat ggccgcatga ccgacgggca
      961 aggacatgtc gtcgatttcc gcaacacggt gatcatcatg accagcaatc tcggcaccga
     1021 gtttggccgc aaaggcggcg cgctgggctt cacacgaccg ggtgtggcgc ccgcgcagat
     1081 cgccgatgcg aagaacatcg aagatcaact gaagcgcaca ttccggccgg agttcctgaa
     1141 ccggatcgac gagatcatca tctttggcac actgtcggtc gaagatgtgg agaagatcgt
     1201 cgatctgcag atgaatgata tccgcagccg cctgcgcgat caagggctgt tcatcgaact
     1261 atccgaagcg gcacgccagt ggctggcgaa ggaaggatat gatccacagt ttggcgcgcg
     1321 accgttgcgc cgcgcactgc agcgccatgt tgaatcgccg ctgtcggtaa agatgctcaa
     1381 gggtgagttt aaagttggcg ataccattat ggttgatgca aacgagagtg gtttagtttt
     1441 tagtcagaag gaaaaggtca gcgtgacgca agatcagcca gcgtgatctc gatctggtaa
     1501 aagtaaatcg ggtgatgatc gaaatttcga tcatcacccg atttttattt tagccgtgtc
     1561 agggtgtgac gtcaggatcg gtttcgatca agattcgtgg atcgccgccg atggccgcgc
     1621 tgccatcgtt cgtcggtgtg ctgtaattgg tcgcggccgg taaggtgatg gtatatacgc
     1681 cattgatcga gaaaattgct tgagtcgatc catccggtga gatcagctcc gcggaagacg
     1741 aagtggcact cagcacgacg gtttcagcga cattgctgcg cgcccacatc acgacgatgc
     1801 gctgaccagc gctagcgcgt ttgaaggcga tcaattcttg attcgaggtg ggccgcttac
     1861 gccaatatgg cacagcgcct aacactcgat tggcaaagac ttgataagtg tcgtaagacg
     1921 atcgggtggt accgtcccgt gcagtttgcg gacaagccgg catactcgcc ggattgcgga
     1981 tcaaaccaaa ggcatcgtaa tgaccatcgt tgccgcagtc atcgaccatc tggaaccaga
     2041 tcgccatttc ggcattcaac cactgcgcga aggtcagggt ttggatcaag taattggctt
     2101 gttcatgcat cgacccgcga taggtacttg gacagaagac gtaaggcgga tcatcgcaca
     2161 cgggcaggcc ggtttcggtc agccataagc ggacgttatt cagaccccgc gcgtttagct
     2221 ggctgcggtc ttgatacaga taattgaagg tctgccacga ccaggcatag ctgtgcgagg
     2281 cgacggcatc catgtagccg tgatacgtga tcgacagtgg atcagtagca atgatgttta
     2341 aggtgtcgtt gagccaagtc ggtttttcaa aatgagccat gccacccaag acaatcaccg
     2401 cgtgcggatc ggcggacttg gccgaaaggt aagccacttt caataaacgc gcataatccg
     2461 tgatcgtgcc gctgaaaaag aaattgagat cctcttcatt ccaaatttcc cagtgagtaa
     2521 tgccggtcgt gcttaaccaa ccctgtgcct gagctattgt cccgccgggc ttgtagcgat
     2581 ttacagccgc attcacaaaa tatgcccagc gattattcgg attgatcggc ttacccggca
     2641 catcgttggt gatgacgccg ttggcataga tgccgtcgta taaaccgatc ggcggactcg
     2701 cgacgctcga aatggtcgtt ggcccgttcg ttgataggct tttctccagc acacgcagac
     2761 catcaccgat cttggggcgc ggggcgagtg cgctgccgcc cgtggcgaga tcgggtggcg
     2821 tgcccaacaa gatcgcatcg atcggcaagt tgtgctgtgt gtctgcgatc acatttgcat
     2881 cgatcttgct ccaatcaaag gtgcgtggtt gagtaatggg ctgcggctca atgccatacc
     2941 aataaaacgg ccagcggttg atcgccggat tgagatcggc ggcctgttga tagcgagtgg
     3001 tattcgcagg cgcttcagct gaactgacaa agttcaagcc caggcgtaac gagaccatga
     3061 caggggttgg tggcgggata taattaacgg cgatgattgg aaaatagatc gtcgtcgtga
     3121 tggtggcggt gcggtgaagt tcagacggat ttatcggcct tcgatccgct gcagcggggt
     3181 gagcgctgga tgcgcttgag ctgctgaggg tgatgcccaa ccacactacg gcaaaccagc
     3241 cgacgacaaa tgatttgacg agtttcaaca tgtgaatagt ttactcgctt tggtctgaag
     3301 attccaggac aactcggtta caattactca acgtttgaat tacgatcgat gtagagtcag
     3361 tgcggtatgc ttgaattata atcagataat caattcacgg gagaaagtat ccatgggtct
     3421 attcgacgga caagtagcct gggtcacggg cgcaggccgc ggcatcggtc gcgcggcggc
     3481 gttggcgttg gccgagcagg gcgcgtcgat tgcgattgtg tcgcgcacgc agagcgaact
     3541 cgatcaaacg gcggacgaaa tcaaagcgcg cggcggcaaa gtgctcacgt cgctgctcga
     3601 cgtgagcaac tgggacatgg ttgaatggac agccaagcag atcgaatcgt tgcttggccc
     3661 gatcgatctg ctgatcaaca acgcgggtgt tttggagccg cttggcaaat tatgggaaac
     3721 ggctccggaa gagtggacgc gcttgatcga catcaatgta tcgggtgcat attattgcat
     3781 gcgggctgtg ctgccgggca tggtcaaacg cgatcgaggt gtcgtgatca atatcagttc
     3841 cggtgcggcg acgaatgcgg cgcccaattg gacggcgtat gccgcctcga aagccgcgct
     3901 cgatcaattg acgcgaacgc tcgccgtcga tctgaaaggc acggcagttc gcacctattc
     3961 actgcacccg ggcattaccg aatcgaatat gcagcacaca ttgcgcaatg cgaccgcgga
     4021 tcaactgccg ccggaacgca gtcaattttt catcgacgaa aaaatttccg gcaatgtgtt
     4081 ggaccctagc gtgcctgctc aggcgatcgt ttggctgtgc tcggcgttat gcgatctcga
     4141 caacggcgcg atgatcgatc tgcgctcaca gccggagtat gtggagaaga tcacacaagc
     4201 gttgaggtag gggcgaggca ttcccaaatg atcaatgttg gccaccgcac aaatgaaatt
     4261 gagattgtag tcaaaaaaga tcgctgtgga atgcctcgcc cctactactt gatcattgca
     4321 gactgttgat gatttgctca aactctgcgc gccgttgctc gaacgcgttt ggcgcggacc
     4381 ataggctgaa ttgcaagatg ccttgcggaa taatcgtgta ggcatctacg ccccgcgtcg
     4441 aattcgccgc acttgaccac tccagccaca aaatattttt tccgtttgag gatcgctgct
     4501 ctggccctac atcgatctga tagtcggcaa aacttccgcg tcgtatctcg ttcgccgctt
     4561 gcgccaactt ctgcacgtca gttgctccgt cgaacaaagc cacagagaag attaaatcgc
     4621 cttgtggcga gatcaaattg agcagttcgc cttgcgcgcc gttgggattg ggagaccaat
     4681 cgggcgggat tgaaatcttc agcgtagttg agacgagatt agtttcgatc caatttggaa
     4741 ccggcgtcga aacgatcgat ggaattgatc ccggttgatt tgctatcagc gttgcatctg
     4801 attttgaaat ctccggcagg cgcggcacga tcaagccgaa agctactgca cagaaggcaa
     4861 tcaacaacca gcgtaattgc gatcggcgct ggatcatgat ccagccgatc gccactccga
     4921 cgatcaatgc ggccgccgta aaccataact ggccgagctg catgccggat cgattgagcg
     4981 cggcagtgct gtattgcgct tgccaatctt gaacaatttg caaaaatgga attggcaaga
     5041 cgagcggttt cagccaaaac acggcgaagc ccaaggccag cgcgccacca gccaccaatt
     5101 gattcaatcg atcttcgcgc tgcagccggt gagatgataa tacgatcgcc cagatgagcg
     5161 gtgcgatcaa taagtgtatt cccgccagcg gattgatccc caagaccacg atcgcgctga
     5221 gactgccgag cgcaaacaaa gccgcgagct taatcccgcg catcggcaca aaatgcgact
     5281 ggttggtcag cagcgtatag agcgccaacg gaattccaag ataaacaatg ccatagaaag
     5341 aatatagagt tgattcttga atgaactgta cggcatttgc cgacagcacg atcgagatga
     5401 cgctcagcat gatcaacaac atcgccagta agctgtacaa acgcgatgag gattcaccag
     5461 accaatcggc gccatgttta gccattcgcc acagaatgcc gccgactaac gccagcgccg
     5521 cccaagcgag caaagattga atcgagcgct gatcgatccc ggcgaaaccc atcaagctgg
     5581 agtgataacc taacacgctc aggtaggccg caatccacaa gccattcgcc caggccaact
     5641 tgttgtcggt ataagtgtga cgcaagcgca ggattattaa ataagccaac gcgatcaaac
     5701 tcacacagac ctgagcttgc acggcaaagt aatcggggtt gctccaagtg ctgacccaat
     5761 ccgtcagtcc gaggtggtac gcgaacacgg cgatcgataa gggcagcggc cccgtcgcca
     5821 gataaccgaa gatcgccacg gccagccacc cgatcggaat gaaccagcgc agccaacggg
     5881 ccgtgacgat ctgcattgct tcacgcgtga gccagcggcc aagaccgagg ctgccggcga
     5941 ataaaccgcc gcccagccac atccaaaata gatcggccgc gcccaaaaga ttctgcatcg
     6001 ccaagttggc attggcggcc gactgggccg catcgcgcgt gtaacttagc gcgaagaagc
     6061 tgccgacgat caggagcgtc aaccagaaga tcgatcgcgg attcttgatc gcgtccaacc
     6121 tcgacgggat cgcccagcga cgcacgatcg gattgaacaa cgcaccaaaa tagattagcg
     6181 caagaatcaa gacgcctgat gccaatggaa ttggtaccca cagatgactt aagccgaaag
     6241 cgccatacgt taaatacgct gcgccgaaac acaagaggaa cgaacacact cgatcgatcc
     6301 gccggatcgg tcggcgcatc acttgccagc cgagaattaa catccacaat gtcggcagcg
     6361 cgaagccgag cgatccggcc aacgctccgc cgatcaggat tccgtaccag ccgagatatg
     6421 cgctgacgat cagatacacg atcggggccg aattgaccgc ggccgtcagc gcgaacgccc
     6481 agccaagggt gaacatcact aagccgataa gtgtcgccgt tgacgattgc cgagtagaga
     6541 acgccgtcgt cgtcagccat gtgccgatca acaacgcgat cagtcccagg cggcttacgc
     6601 tgctgaccgt ccacagacgt cgagccagat agaaaccttt gagttcacct tgcag
//
LOCUS       DASRTJ010000078         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_342499,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000078 DASRTJ010000000
VERSION     DASRTJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342499"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1768)
                     /locus_tag="VFF70_01510"
     CDS             complement(230..1768)
                     /locus_tag="VFF70_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY43398.1"
                     /translation="MPQRSSNRIIALVCVAIFIGAMDLTVASAFLPQVVVDFELSTQQ
                     FALAGWVVTMYLAAYAVSMTFAGRLSDLYGRRSAYLVCLGIFVFGSLAVALTRTPGLQ
                     PSFSLEWLVIARVIQALGAGAMVPVSMALVADLFTPDRRATPLGFIAAVDTAGWVVGH
                     LYGGIMIRLFTDWRIIFWFNIPIGIIAFIVCARVLKNVTQPKSSGKLDSIGAILIGAA
                     LAAFNIALGAGAESPQVSQFVDAVTPAYQVPLLIASIGLFVGFIGWELRSKQPLLDLR
                     LFRNRTFTGAALVNLGIGFALIVALGDVPLFINAVVASGNTNTSVAEILSDGAWFTGW
                     VLSALTLTMAIMSLFIGRIVNRFGYRAPTLIGLAIATIGFWITSQWQVTTTYLEMTPG
                     LIIAGAGFGMILSPIATAVVNNAPDQERGSASALVIILRLVGMSLGGSIALTWGTQRV
                     QTLVAQFAAGSNQFAGNPLEFFRRATSQAVNESFVLFAVLACAVALLPALLMRKENLD
                     AVYD"
BASE COUNT          481 a          516 c          511 g          354 t
ORIGIN      
        1 aatacgatcg cggcgatctt gaggatcagg ccgcgcggac cgggcttggg gagatgaact
       61 tcaaatacat tcgtttcggg tagtgggtcg ttcatgcttg tcctcctgac aaatgatacg
      121 cttgttccat tatctaaccg atcttgatta tagatcaagc actcaagtgc atgcttttcg
      181 gcggctgaaa gtcacatgcg atcagataag aatagaacaa tctgcgtctt cagtcataaa
      241 ctgcgtctaa attttctttt cgcattaata acgcgggcaa cagcgcaacc gcgcacgcga
      301 gtaccgcgaa caacacgaac gattcattga cggcctgact cgtcgcgcgg cggaaaaatt
      361 cgagagggtt gcctgcgaat tgattcgatc cggcggcgaa ctgcgcgact aaagtctgta
      421 cgcgctgagt gccccacgtc aacgcgatcg atccgccgag gctcatgccg accaagcgca
      481 agatgatcac cagggcggag gcactgccgc gttcttgatc gggcgcgttg ttgacgaccg
      541 ccgtagcaat cggcgacaag atcataccga agcccgcgcc cgcgataatc aagcccggcg
      601 tcatttcaag atacgtggtt gttacttgcc attgactcgt gatccaaaaa ccgatcgtgg
      661 cgattgccaa gccgatcaac gttggcgcgc gataaccgaa tcgattaacg atgcggccga
      721 tgaacagtga catgatcgcc atcgtcaagg tcaacgcgga caagacccag ccggtgaacc
      781 acgcgccgtc gctgagaatt tctgccacgc ttgtattcgt gttgcctgat gcgacgacgg
      841 cattgatgaa caatggcaca tcgcccaagg cgacgatcaa tgcaaagccg atgccgagat
      901 tcactaaagc cgcgccggta aacgttcgat tacggaacag acgcagatcg agcaggggtt
      961 gtttcgatcg gagttcccac ccgatgaagc cgacgaacaa gccgatcgag gcaatcaaca
     1021 gcggaacttg atacgcgggt gtaaccgcat cgacaaactg cgaaacttgt ggcgattccg
     1081 ctcccgcacc taaggcgata ttaaatgcgg ccaaagctgc gccgatcaag atcgccccga
     1141 tcgagtcgag cttgcccgac gattttggct gcgtcacatt ttttagcaca cgggcacaca
     1201 cgatgaaggc gatgatcccg atcggaatat tgaaccaaaa aatgatccgc caatcggtga
     1261 acaagcgaat catgatgccg ccatacaaat gcccgacgac ccagccggct gtatcgaccg
     1321 cggcaatgaa gcctagaggt gtagcccgcc gatcaggtgt gaatagatcg gcgacgagcg
     1381 ccatcgatac cggcaccatc gcgcccgcgc ccagcgcttg aatcacgcgc gcgatcacca
     1441 accattcgag cgagaaggac ggttgaagtc cgggcgtgcg cgttaaagcc accgcgagtg
     1501 atccgaaaac aaaaattcct aaacatacca agtaagcaga tcgacgcccg tagagatcgc
     1561 tcaatcttcc ggcgaacgtc atgctcacgg catacgcggc gagatacatc gtcaccaccc
     1621 aaccggccag ggcaaattgc tgcgtgctca attcaaaatc gacaacgacc tgcggcagaa
     1681 acgctgatgc cacggtgagg tccattgcgc cgatgaagat ggcgacgcag acgagcgcga
     1741 tgatgcggtt agaggagcgt tgcggcatgc gagtcctcgt caagaggcag gatgcaagtt
     1801 gcaggcagtg agtactgtca tttcgacgag cggcgcgagg agaaatctct atctctgtta
     1861 ag
//
LOCUS       DASRTJ010000079         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307065,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000079 DASRTJ010000000
VERSION     DASRTJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307065"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFF70_01515"
     CDS             <1..287
                     /locus_tag="VFF70_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43399.1"
                     /translation="HTLYVLDEPSVGLHAADVHKLIDVLNRLVDSGNSVLIIEHHPDI
                     IKVADHLIDLGPEGGQRGGYIVATGTPEEIAANKESHTGQFLKKHLQPKR"
     gene            601..1887
                     /locus_tag="VFF70_01520"
     CDS             601..1887
                     /locus_tag="VFF70_01520"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZY43400.1"
                     /translation="MNESKNPFETAQAQFDQGADLLGLTQDAKDLMRWPLREFHFRIP
                     VRMDDGTVKVFNGFRVQHSDARGPSKGGIRFHPNETIDTVRALAMWMTWKCAVADIPL
                     GGGKGGIIVDPSSLSIHEKERLCRGWVDQMWRNIGPRMDVPAPDVGTTPQMMAWMMDE
                     YSKLSGQYTPGVFTGKPVDMGGSLGRTEATGYGVIYTVREAMKHLKIDPKTSVAAIQG
                     FGNVGQYAAIGFNEILGGKVACVSCYDAKAKKPYTYSHPNGIDPVFLQSITDEYGTID
                     PAQAKTAGYIQEDAMAWIGKEADVLIPAALEGMITGETAPKIHARVKIIAEGANGPTT
                     LEGDEVLKKRNIFNIPDFLCNSGGVTVSYFESVQNDMNFYWSREEVLQRLDHKITVAF
                     GHVLDMALDRKVYMRDAAYLVAIDKVVRAMRLRGWV"
     gene            2014..>3672
                     /locus_tag="VFF70_01525"
     CDS             2014..>3672
                     /locus_tag="VFF70_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZY43401.1"
                     /translation="MSQPLTEPPNILAIYLQIAQYPILAKDIHGRMRAELFTRGIINS
                     DRLEEEVKAKAILSQQREGISDPLNDEAEQTWEQRRQLIRDYLTDFYFAYNLPLDVFT
                     NIVNELLAQRMGHPATGDLPFNPELAPIDLLLTKAQQYESMPEEYQAKVRHHLEEIIV
                     VLIKTMISDQLAFVRVAKQWFTAEDFRSIRSRLIGSGKIGGKAAGMLLAHKILQHAAP
                     SIAERTTVPDSYFIGADVFYDFLSLNKLQYNQKYKIADQIQAEYPHIRDAYEAGRFPD
                     EIAEQLRGILTKVGQTPLIVRSSSLLEDNFGTSFAGKYESFFCANQGTPKENLRALTT
                     AIRRIYASVYSPDALFYRRHMGLLDYDERMAILLQTVEGQSYQHYRFPTLAGVAFSRS
                     PIVWSPRLRREDGFVRLVLGLGTRAVNRVADDYPRLITLSHPLLRPETTLLAISRYSQ
                     HFIDMIDVQANAFVTRSVRETIGRDYPALRWIASVNEDDTLLPIMSLGLSVSPDRLII
                     TFDNLLQRTDFTSTLKEMLATLAKHYNFPVEMEFAASITGSDVSH"
BASE COUNT          879 a         1029 c          947 g          817 t
ORIGIN      
        1 atcacacgtt gtacgttctc gatgagcctt cagtcggttt gcatgccgcc gatgtgcaca
       61 aactgatcga tgtattgaat cgactggtcg attccggcaa cagcgtgttg atcatcgagc
      121 atcatcccga cattattaaa gtggcggatc acctgatcga tcttgggccg gagggcggtc
      181 agcgcggcgg ctacatcgtg gccacgggca cgccggaaga gatcgccgcg aataaagaat
      241 cgcacaccgg gcagtttttg aagaagcatc ttcagccaaa acgctaataa gttctgatgc
      301 cataatatca gaagatgaaa ctgcccaacg cctcagagtg atgaaactca tcgcagaatc
      361 atcacattta gcaccggagt attttgacag tttgcgctat tctaatgtag aatgaacgtt
      421 atcagagcat tcctatgaac cacttaacat gtctaatcga tgccgccctt cgattgagcg
      481 tgggcaaccg cgaagggacg cattacagct cgtggataaa agcgaccaaa ctcggtcgct
      541 tttttgtcgc aacctaacgg cgatcgatct cgccgtcaaa tttaatcagg agagataact
      601 atgaatgaat cgaagaatcc cttcgaaacc gcccaagccc agttcgatca aggcgcggat
      661 ctgctgggtc tcactcagga tgccaaagac ttgatgcgct ggccgctgcg tgagtttcat
      721 ttccgtattc ccgtgcggat ggatgacggc acagtgaaag tttttaacgg cttccgcgtt
      781 cagcacagcg atgcgcgcgg cccgtccaaa ggcggcatcc gctttcatcc caatgaaacg
      841 atcgacacgg tccgcgcgct ggccatgtgg atgacatgga agtgcgcggt cgccgacatt
      901 ccgctcggcg gcggcaaagg tggcatcatc gtcgatcctt catccttatc gatccacgag
      961 aaggaacgcc tgtgccgagg ctgggtcgat caaatgtggc ggaacattgg tccgcgcatg
     1021 gacgtgcctg cgccggatgt cggcacaacg ccgcagatga tggcttggat gatggacgag
     1081 tacagcaagt taagcggcca atatacgcct ggcgtcttta ccggcaagcc ggtcgatatg
     1141 ggcggctcac tcggtcgaac ggaagcgaca ggctacggcg tgatctacac ggtgcgtgaa
     1201 gcgatgaagc atctcaagat cgatccaaag accagcgtcg cggccattca aggtttcggc
     1261 aacgtcgggc aatatgccgc gatcggcttc aacgaaattc tcggcggcaa agttgcgtgt
     1321 gtgtcatgct acgacgccaa ggccaagaag ccctacacgt attcgcatcc aaatggcatc
     1381 gatccggtct tcttgcaatc gatcaccgat gagtacggca ccatcgatcc tgcgcaagca
     1441 aagacggccg gctacattca agaagacgcc atggcttgga tcggcaaaga ggctgacgtc
     1501 ttaattccgg ccgcgctcga aggcatgatc actggcgaaa ccgcgcccaa gattcatgcg
     1561 cgcgtcaaga tcattgctga aggcgcgaac ggcccgacga cgctcgaagg cgatgaagtt
     1621 ttgaagaagc gcaacatctt caacatcccc gacttcttgt gcaacagcgg cggcgtcacc
     1681 gtttcgtatt ttgaatcggt gcagaacgat atgaacttct actggagccg cgaagaggtg
     1741 ctccagcggc tcgatcataa gatcaccgtg gccttcggcc acgtgctgga tatggcgctc
     1801 gatcgaaaag tgtacatgcg cgatgcggcc tatctcgtgg caatcgataa ggttgtccgc
     1861 gcgatgcggc tgcgcggctg ggtgtaaacc cgatcgatat cgctaacaaa atcaatccct
     1921 ctcgtcgatc aggctgactg gcgagaggga ttttcttttg tcatttgtaa tttgtcattg
     1981 ttcattgtcc cttgttaatt gtataatctg cccatgtctc agcccttgac cgaaccaccc
     2041 aatattctgg ccatttattt acaaattgcg cagtatccca ttctcgcgaa agacatccac
     2101 gggcgcatgc gcgccgaact ttttacgcgc gggatcatca actcggaccg gctcgaagaa
     2161 gaagtaaaag cgaaagccat tctctcccag cagcgcgaag gcatttcaga tccgctgaat
     2221 gatgaggccg aacagacctg ggaacaacgc cgccaattga tccgagatta cctgactgac
     2281 ttttattttg cgtacaacct cccgctcgat gtctttacga atatcgtcaa tgagctgctc
     2341 gcgcagcgca tgggtcatcc agccaccggc gacctgccct tcaatcccga acttgccccg
     2401 atcgatctgc tgttgaccaa agcccagcag tatgaatcga tgccggagga atatcaggcc
     2461 aaggtacgcc atcaccttga agagattatc gtcgtcttga tcaaaacgat gatcagcgat
     2521 caactcgcct tcgtgcgcgt cgccaagcaa tggttcaccg ccgaagattt ccgttctatc
     2581 cgatcgcgcc tcatcggcag cggcaagatc ggcggcaagg cggcgggtat gctgttggca
     2641 cacaaaattt tgcagcacgc cgcaccgtcg atcgccgaac gcaccacggt gccagattcg
     2701 tacttcatcg gcgcagacgt cttctacgat ttcttgtcgc tgaacaaact tcaatacaat
     2761 caaaaataca aaatcgccga tcaaatccag gccgagtatc cgcacatccg cgatgcgtac
     2821 gaagccggtc gattcccgga cgagattgcc gagcaactgc gcggcattct cacgaaggtc
     2881 ggccagacac cgcttatcgt ccgctcatca agtttactcg aagataattt cggcacatcc
     2941 ttcgcgggca aatatgaaag tttcttttgc gccaatcaag gcacacccaa agaaaatctg
     3001 cgcgccttaa ccaccgccat tcggcgcatc tatgccagcg tctacagtcc ggacgcctta
     3061 ttctatcgac gccacatggg cttgctggat tacgacgaac gcatggcaat tttgctgcaa
     3121 acagttgaag gccaaagtta ccagcactat cgctttccaa cattggccgg ggtggctttc
     3181 agccgcagtc cgattgtgtg gagtccgcgc ttgcgccgtg aagacggatt cgtccgttta
     3241 gtcctggggc ttggcacgcg cgccgtcaac cgtgttgccg atgactatcc acgcttgatc
     3301 accttgagcc atccgctcct gcggcctgaa acaacgctcc tggccattag ccgctactcg
     3361 cagcacttca tcgacatgat cgatgtacaa gccaatgcat ttgtcactcg atcggtcaga
     3421 gaaacgatcg ggcgtgatta tcccgcgctg cgctggatcg catctgtcaa tgaagacgat
     3481 acattgctgc cgatcatgtc gctggggctc agcgtgtcac ccgatcgatt gatcatcacc
     3541 ttcgacaatt tattgcagcg caccgatttt acgtcgacac tgaaagagat gttagccacg
     3601 ctggccaagc attacaattt cccggtcgaa atggaatttg ccgcttctat tacgggcagc
     3661 gatgtctcgc ac
//
LOCUS       DASRTJ010000080         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_4458,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000080 DASRTJ010000000
VERSION     DASRTJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4458"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1647
                     /gene="der"
                     /locus_tag="VFF70_01530"
     CDS             217..1647
                     /gene="der"
                     /locus_tag="VFF70_01530"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZY43402.1"
                     /translation="MTKPFVALVGRPNVGKSTLFNRLVGERLAVVDEVPGTTRDRLHA
                     DSTWNGHTFRVIDTGGIDVSDRSAKADRPLNPNTSVEQASIEFLPMIRAQAEIAIQES
                     DAVIFVVDAEAGLTNADREVAEILRRYQLERNGQPWPPIFLAANKADNPDRRNASLEF
                     YELGLGQVYAISAQHGLELGDLLDAVVQSFPPIEVEPEDESVKISIVGRPNVGKSSLL
                     NALLGQERAIVSPVPGTTRDAIDTPMTWGGLAVTLIDTAGIRKRGHIDPGVEQYSVLR
                     ALRAIERSDVALLVIDAVEGVTAQDAHVAGYVLEENKSVVIVINKWDMLQPGLQPKGD
                     GSLTPKEKEFSLTIRTALNFLDYVPVMFVSAKTGLRVDEVMAMALRVQEERLTRIPTS
                     ELNRILREAMDKQAPPTKAGQRLKIFYASQVRVDPPTFLFHVNDSRLVHFTYERYLEN
                     QIRSAHPFVGTPLRLSFRNRARAELE"
     gene            1647..2777
                     /locus_tag="VFF70_01535"
     CDS             1647..2777
                     /locus_tag="VFF70_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43403.1"
                     /translation="MDQFIAVAHNPVQVDTSARNRFIRQAGILYGAIFAIGFSFALWG
                     PDALALQQAFVYLWWGKFVIGLVLILPVCLFAGWLSAGGGLSAVGIGVWIVGCPLIIL
                     IAAHVPYDGVSWLASLNDPYPTAQVMYPFTAPTAAITGLCMLFGAGVGLFMGLISLFA
                     IGQAWDRSTRSNRLSLRSILLLSLSLIPIVPLAPIVDNYIDAPRRFPLVDVQTVIDLA
                     LDPRVNLDSLRLPFLKNLRGDLSANYILMWVSSTADEQMSNIDVQFDNGLVLRCGYNF
                     GSVGLCTRLNQNLDKWMTELMTTGHLSCAGCSVRTDRVTRRWLAATLPTLNGSASLLT
                     VSLLKHQSGWVYERAVFDNGQMIDCRFSGDSPIIVDLCIEAR"
     gene            complement(2861..>4974)
                     /locus_tag="VFF70_01540"
     CDS             complement(2861..>4974)
                     /locus_tag="VFF70_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43404.1"
                     /translation="LTEQARWNAAAMKLRLGQTSAAVADYLQFTQQYSDTTYLPDTWF
                     ELGEAYFAQSDWSKSIGAYQKYLKLRGDLIGSYVQERLGDAYSNLKDFENAAMAYEKS
                     IQQASSSSAEAGLREKLALSYRLLQRTDEAIVQYTSILSFAQNSAYQAQVIYQLGQTY
                     LDAGRTTEGYAQFQQLVNFYFDRGDAYQALVVLLDANQDVNEFQRGLVDYYAKQSDAA
                     IAAFNRFIQMTTDHGNAHYYSGLAYRSAGNIGAAVKAFDAAITHPEASHWGDAWIEKA
                     NTQAIGGDVTGAIDTLTSFVKQHPIDPLAPTALWRAAGILESSGDYQRAIDLNLQLQI
                     NYPSDQNASESLYDAGLDAYRVDDMHVAITAWQTLSNTYPLSNLYAGSLLWQGKALQK
                     LGQDQQANQLLSFAAQATIDYFGIRAQDVLSNSSGLPIISAQLNIDPNEGRATAETWL
                     AKWLGVSVTALQSLPVPVIADGRFQRGEVFWVLGRFDRARDEFESLRQSYSDNAAVLY
                     PLAIYFRDIGLYRSSILAAGQVIRLSPAGTSDQAPIFLARLYYPIYYANLVAPEAQDR
                     SVDPLVVYALMRQESLFEGIATSSAFANGLMQIIPGTAEEIAHDLKWPNYQLSDLYRP
                     LISVKFGVYYLAKQRDYLDGDLIAAWAAYNGGPGNAKRWRDAANGDVDLFVENISLTE
                     TRLYIERLRENLAMYQRLYGK"
BASE COUNT         1100 a         1396 c         1336 g         1142 t
ORIGIN      
        1 ctgcccgcga caattcaagt cagcattagt gagatcatca caccaaccga aacgattaca
       61 ccaacacgca ctttaacccc gacgccaaaa ccgtaaacat ctttttgtca ttctgagccg
      121 cgcagcggcg aagaatctcg gccaataatg cgagaccctt cgcggagttt ccactgatcg
      181 aaatcgaagt gctcagggtg acacgatcga gtattcatga ccaaaccatt tgtagcctta
      241 gtcggccgcc cgaatgtcgg caaatcaact ttgttcaatc gcctcgtcgg tgaacgcctg
      301 gccgtcgtcg atgaagtgcc cggcaccaca cgcgatcgat tgcatgccga cagcacgtgg
      361 aacggtcata ccttcagagt gatcgatacg ggcggcatcg acgtgtccga tcgatccgcg
      421 aaagccgatc ggccgttgaa ccccaacacc tcggtcgagc aagcctcgat cgaattcctg
      481 ccgatgatcc gcgcacaggc cgagatcgcc atccaagaat cggacgcggt gatcttcgtc
      541 gtcgatgcgg aagcgggtct caccaatgcc gatcgagaag tggccgagat attgcgccga
      601 tatcaattgg agcgcaacgg gcagccgtgg ccgccgatct ttctcgcggc gaacaaagcc
      661 gacaacccgg atcgccgcaa tgcttcgctt gaattttacg aactgggtct tggccaagtg
      721 tatgccatct ccgcgcagca cggcctggag ctgggcgatc tgctggatgc ggtcgtgcag
      781 tcgtttccgc cgatcgaagt tgaaccggaa gatgaatccg tcaagatttc gatcgtcggt
      841 cggccaaacg tcggcaagag ttcgctgttg aatgcgctgc tcggccagga acgcgcgatt
      901 gtcagtccgg tgcccggcac cacgcgcgac gcgatcgata cgccaatgac atggggcggc
      961 ttggccgtga cgctgatcga tacggcgggc atccgcaaac gcggccatat cgatccgggc
     1021 gtggagcaat atagtgtgct gcgcgcgttg agggcgatcg aacgatcgga tgtggccctg
     1081 ctcgtgatcg atgcggtgga aggcgtgacc gcgcaagatg cgcacgtcgc cggctacgtc
     1141 ctcgaagaaa acaagagcgt tgtcatcgtc atcaacaaat gggacatgct gcaacccggc
     1201 ctgcaaccga agggagatgg ctcgctcacg ccgaaggaaa aagaattcag cctaacgatt
     1261 cgcacagcgt tgaacttttt ggactacgtg ccagtaatgt ttgtgtcggc gaagacgggg
     1321 ctgcgggttg atgaagttat ggctatggcg ctgcgtgtgc aagaagaacg actgacgcgc
     1381 atcccgacca gcgaactcaa tcgcatcttg cgcgaagcga tggacaaaca agcgccgcca
     1441 accaaggccg gacaacgctt gaaaattttc tatgcctcgc aagtgcgcgt tgatccgccg
     1501 acgttcctat tccatgtgaa cgattcacgc ctggtgcatt tcacgtacga acgctatttg
     1561 gaaaatcaga ttcgttcggc gcatccgttt gtcggcacgc cgctgcgctt gagttttcgc
     1621 aatcgcgccc gcgctgagct ggaataatgg atcaattcat cgccgttgct cacaacccgg
     1681 tacaggtcga tacttcggcg cgcaatcgct tcattcggca agccggaatt ctttacggtg
     1741 caatcttcgc cattggtttt tcattcgcgc tctggggacc ggacgcgctc gcgttgcaac
     1801 aagccttcgt ctatttgtgg tggggcaagt tcgtcatcgg tttagtgttg atcctgccgg
     1861 tgtgtttgtt cgctggctgg ctgtcggccg gcgggggcct atcggcggtc ggcatcggcg
     1921 tgtggattgt gggctgcccg ctgatcattt tgatcgccgc ccatgtgcct tacgacggag
     1981 tcagctggct agcgagcctc aatgatccgt atcccactgc ccaagtcatg tatccgttta
     2041 cagcacccac cgcagctatt accggtctat gcatgctgtt cggagcaggc gtcggcttgt
     2101 ttatgggctt gatctcgctc ttcgcgatcg gtcaggcctg ggatcgatcg acgcgctcca
     2161 atcgcttaag cctgcgttcg atcttgctgc tcagtctgag cttaatcccg atcgtgccgc
     2221 tggccccgat cgtggataac tacatcgatg cgccacggcg ttttcctttg gtggatgtcc
     2281 agacggttat tgatctggcg cttgatccac gagtcaactt agattcgctg cgtttgccat
     2341 ttttgaaaaa cctgcgcggc gatttatctg cgaactacat tttgatgtgg gtctccagca
     2401 ccgccgatga gcagatgtcc aacatcgatg tccagttcga taacggctta gtgctgcgct
     2461 gcggttacaa cttcggctcg gtgggcctgt gcacgcggct taatcagaac ctggacaaat
     2521 ggatgaccga gttgatgaca actgggcatc tcagctgcgc gggctgctcc gtgcgcaccg
     2581 atcgagtcac gcgccgttgg ttggccgcga ctttgcctac gctgaacggc tctgcgtcat
     2641 tgctcactgt cagcctgctc aaacatcaaa gcggttgggt ctatgaacgg gccgtcttcg
     2701 acaacggcca gatgatcgat tgtcgcttca gcggggacag tccgattata gtggatttgt
     2761 gtattgaagc caggtaatca tcaacgggca tgaagcagac aatcggattg aaacggatga
     2821 cttaatcatt tgtcattgtt cattgtttat tgttaattgc tcatttgccg tacaatcttt
     2881 ggtacatcgc cagattctcc ctcagccgtt cgatatacag ccgcgtctca gtcagtgaaa
     2941 tattctccac aaacagatcg acatcgccat tggccgcatc ccgccagcgc ttcgcattgc
     3001 ccggcccgcc gttgtaagcc gcccacgccg cgatgagatc gccatccaag taatcgcgct
     3061 gcttcgccag ataatacacg ccgaacttca cgctgatcag cggtcgatac aaatcactga
     3121 gctgatagtt tggccacttt agatcatgcg caatctcttc tgccgtgccg gggatgatct
     3181 gcatcaagcc attggcaaac gccgacgaag tggcaatgcc ctcaaacaaa ctctcctgcc
     3241 gcatcaaagc ataaaccacc agcggatcga ccgatcgatc ttgcgcttcc ggcgccacca
     3301 ggttcgcgta atagatcgga taatacagcc gtgccaaaaa gatcggggct tgatcggacg
     3361 tacccgcggg cgacaagcga atgacttgcc cggcggccag gatcgacgat cgatatagac
     3421 cgatgtcgcg gaaatagatc gccaacggat acaacacggc ggcgttgtca gaataacttt
     3481 gtcgcagaga ttcaaactca tcacgcgccc gatcgaatct gcccagcacc caaaatactt
     3541 cgccgcgctg aaaacgccca tccgctatga cgggcaccgg caatgattgg agcgccgtta
     3601 cactcacgcc cagccatttc gccagccagg tctcggctgt cgcgcgtcct tcattcggat
     3661 cgatattcaa ttgcgctgag atgatcggca gtcccgacga attgctcaat acatcttggg
     3721 cgcgaatgcc aaaataatca atcgtagctt gcgccgcaaa ggataagagt tgattggctt
     3781 gttgatcttg tccaagtttt tgcagcgctt tgccctgcca caataacgaa ccggcataca
     3841 gattgcttaa tggataggtg ttgcttaaag tttgccacgc agtgatggcc acgtgcatat
     3901 catcgacgcg gtacgcatcg agtccggcat cgtataaaga ttcagaagca ttttgatcgg
     3961 acggataatt tatttgcaac tgcagattga gatcgatcgc gcgctgatag tcgccgctgc
     4021 tttccaaaat tccagctgcg cgccagaggg ccgtcggggc cagcggatcg atcgggtgtt
     4081 gcttgacgaa cgaggtcagc gtatcgatcg cgcccgtcac atcgccgccg atcgcttgcg
     4141 tgttcgcttt ttcgatccac gcatcgcccc aatgcgaggc ctcgggatgc gtgattgccg
     4201 catcgaaggc ttttaccgcc gcgccgatat tccccgccga gcgatacgcc aggcccgaat
     4261 aataatgcgc gttgccgtga tcggttgtca tttgaataaa tcgattgaat gcagcaattg
     4321 cggcatcaga ctgtttggcg tagtaatcga cgaggccgcg ctggaattca ttcacgtctt
     4381 gattcgcatc gagcagcacc actaacgctt gatacgcatc gccgcgatcg aaatagaagt
     4441 tgaccaactg ctgaaattga gcgtaaccct ccgtcgtgcg tcccgcatca agataggttt
     4501 gaccgagttg ataaatcact tgcgcttgat aagcggagtt ttgcgcgaac gacaagatgc
     4561 tggtgtattg cacaatggcc tcatcggtgc gctgcaacaa gcgataactt aaggccaact
     4621 tctcgcgtaa tccagcctca gcggacgagc tgctggcttg ttgaatcgat ttttcatagg
     4681 ccatcgcggc gttttcaaaa tctttgagat tcgagtatgc atcgccgagg cgttcctgca
     4741 catacgaacc aatcagatcg ccgcgcaact tcaaatattt ttggtaagcg ccgatcgact
     4801 tcgaccaatc ggactgcgcg aaatatgctt cacccaattc gaaccacgta tccggcaaat
     4861 atgttgtgtc cgaatactgc tgtgtgaatt gcaaataatc tgccaccgca gccgacgtct
     4921 gccccaaacg cagtttcatc gcagccgcgt tccagcgggc ttgctcagtt agat
//
LOCUS       DASRTJ010000081         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307153,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000081 DASRTJ010000000
VERSION     DASRTJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307153"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFF70_01545"
     CDS             <1..460
                     /locus_tag="VFF70_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY43405.1"
                     /translation="GLSGYQNQSEINEQMNAAIHAAYDKGDKAQAVELSLQLWTDGPD
                     RAPGHIDPMVREKVRAMTAEVFDLPDYDNSFIQALEPPAIDRLAEIHVPTLFIVGDQD
                     VPDILDIAEIVTTHVPGAQKAIIQNTAHHLNLERPAEFNDIVLDFLKSLQ"
     gene            576..2840
                     /locus_tag="VFF70_01550"
     CDS             576..2840
                     /locus_tag="VFF70_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZY43406.1"
                     /translation="MSSFIRSRILLIGVMTILVVIGAVWVFGPGAVQPIAQIPTLAAA
                     PEITFTPPPDLSTLAAQFPRLAKILNNPELDSAYKEFLLAFERGGADAAILLAQQRGL
                     LTEDNQVRITLVLDTQDSAALVTELTNLGVNVRGTYQDLIDISIPIELIVKTAQSDDP
                     GKVFDQITQLNHVTGLQLPQVKPQNSSDLLKADESPPYYLPLASQPAGANFIDPITSE
                     GVKFIQADVWHAAGYTGHGVKIGVLDQGFDSYRDLLGRELPITVTVKSFVPGIDLGQT
                     GINHGAAVAEIIHDVAPDAEIFLAYYDGGDVSMGNAVDWLVSQGVQIISHSAGGMAAP
                     MDGTGRDAEIVDRVTSAGILWINSAGNSANEHYRGAFTDTDNDGVHEYAPGKTLLGFQ
                     STGGATTQIVLDWNDWSVTGTQALNLFLLDDQGSVLASSRNTREGGHPPVQQIIFKFD
                     ASRTYFVTIQGVNVTQPIRLDLYVHPTLNMELSDPTGSLATPGDAIGALSIGAVNWRN
                     SVLEPFSARGPTADGRIKPDLVGPDGVSNAVNAPDRFYGTSAAAPHIAGAAALIWGAY
                     PQASVADVRQVIISSTIDLLEPGADPLTGYGELQLPQPPPTPTPLPPTSTPAPGITIT
                     PAPTSTPIKVVIAIPTIIRSRPPINSGSSDQSGLILIGIAVITIGLAGSIGFRSINRS
                     RTRSKSIKRSSKPDRSIQLTCTHCGRVNRATAIYCTQCGRPIGATVTATKCAQCDHVL
                     RPRAKFCSNCGKAA"
     gene            2948..>3679
                     /locus_tag="VFF70_01555"
     CDS             2948..>3679
                     /locus_tag="VFF70_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY43407.1"
                     /translation="MTLDRRIEVLRSAFPTMGDELLVYLAHNAREVHTSAGDRICEEG
                     ETGDAFYVIMAGRAQVSKFMTLGAQRFLTELQPGQFFGELSLVDDAPRVASVDALTEC
                     ELLVITKDDFLNLLQDSPSMAVPIMRSVTARLRDSDQRTINELRDKNDQLAAAYAELE
                     YTVKRKSDFLTNVAHELRTPLTSVKGYAHLMRTGMLHGPDLDRAFAAIIDNSEAIVRL
                     VNNILFLQEIDFITPAHDPVDMIQLI"
BASE COUNT          841 a         1058 c         1016 g          764 t
ORIGIN      
        1 cggtttaagc ggttatcaaa atcaatctga aatcaacgaa cagatgaatg cagcgatcca
       61 tgccgcttac gataaaggtg ataaggccca agcggttgaa ctctcgttac aactatggac
      121 tgatggcccg gatcgcgcgc ccggccacat cgatccgatg gtgcgcgaga aagtccgcgc
      181 gatgaccgcc gaagtgttcg acctacccga ttacgataat agtttcattc aagcccttga
      241 gccacccgcg atcgatcgct tggcggagat tcacgtgccc accttgttca tcgtcggcga
      301 tcaagatgtg ccggatatcc tagacatcgc ggagattgta acgactcacg taccgggcgc
      361 gcagaaggcg atcattcaga acacagcgca tcatctcaac ctggaacggc ccgcggagtt
      421 taacgacatc gtgttggatt tcttgaagag tcttcaatag tacatatcat tgcgagccgc
      481 ttcaggcggc gaagcaatct gcgcgacagt aaacgagatt gcttcgtcgg tctgcaacct
      541 cctcgcaagg acgatcgaaa cactcgtcat tcatcatgtc ctccttcatt cgatcccgca
      601 ttctactcat cggcgtcatg acgatcctcg tcgtgatcgg cgcagtgtgg gtctttgggc
      661 cgggtgcggt tcagccgatc gcgcaaatac caaccctggc ggccgcgccg gaaataacct
      721 tcacgccccc gcccgatctc agtacactcg ccgcgcagtt tccgcgcctg gcaaaaatac
      781 taaacaatcc cgaactcgat tcggcttata aagaatttct actagctttt gagcgcggcg
      841 gcgcagatgc ggcgatcttg ttggcgcagc agcgtggatt attgactgag gacaatcaag
      901 tccgcatcac gctcgtgttg gacacgcagg attcagcggc gctggtgaca gaactaacga
      961 acctcggcgt gaatgtgcgc ggcacttatc aagacttgat cgacatcagc atcccgatcg
     1021 agttgatcgt gaagaccgcg cagtcagatg atccgggcaa ggtcttcgac caaatcacac
     1081 agttgaatca cgtcacaggt ttgcagctgc cgcaagtgaa accgcaaaat agcagtgact
     1141 tgcttaaggc ggacgaaagt ccgccgtatt atttgccact ggcttctcag ccggcgggag
     1201 caaactttat cgatcccata accagtgaag gggtcaaatt tattcaggcc gatgtatggc
     1261 acgccgcagg ttacaccggc cacggcgtta agatcggcgt gctcgatcaa ggctttgaca
     1321 gttaccgcga tctgttaggc cgcgagctgc ccatcacggt caccgtcaaa tcgtttgtgc
     1381 cgggcatcga ccttggacag accggcatca atcacggcgc ggccgtcgcg gaaatcattc
     1441 acgatgttgc gccggacgca gaaatatttc tggcctatta cgatggcggc gatgtcagca
     1501 tgggcaacgc ggtcgattgg ctggtctcgc agggcgtgca gattatttcg cattcggcgg
     1561 gcggcatggc cgcgccgatg gacggcaccg gccgcgatgc ggaaattgtc gatcgcgtga
     1621 cgagtgccgg catcttatgg atcaactcgg caggcaacag tgccaacgag cactatcgcg
     1681 gcgcattcac cgacaccgat aatgatggcg tacacgaata tgcgccgggc aaaacgctgc
     1741 tcggttttca atcgacgggc ggcgcgacca cccagatcgt tttggattgg aatgattggt
     1801 ctgttactgg aacgcaggcg ctgaatttat ttttgctcga tgatcaaggc agcgtcctgg
     1861 cttcatcacg caacacgcgt gaaggtggac atccgccagt gcagcaaatc atttttaagt
     1921 ttgacgctag ccgcacgtac ttcgtcacga ttcaaggcgt caatgtcacg cagccaatac
     1981 gcctggattt atacgtgcat cccaccctga acatggagtt gagtgatccc acgggcagtt
     2041 tagccacgcc cggcgatgcg atcggtgcgc tgtcgatcgg cgcggtgaat tggcgcaaca
     2101 gtgtgctgga acccttcagc gcgcgcggtc ccacagccga cgggcgcatc aagccggatc
     2161 tcgtcgggcc ggacggcgta tcgaatgcgg tcaatgcacc cgatcgattt tatggcacat
     2221 ctgcggccgc accacatatc gcgggcgcgg cggctttgat ctggggtgca tatccgcaag
     2281 ccagcgtagc tgatgtgcga caggtcatca tctccagcac gatcgatctg cttgagcctg
     2341 gcgcagatcc actaacgggc tatggcgaat tgcaactccc ccagccgcca ccgacaccca
     2401 cgccgttgcc gccaacctcg acgcccgcgc ccggcatcac gatcacgccc gcacccacct
     2461 ccacgccgat caaagtggta attgcaatac caacgatcat tcgatcgcgt ccgccgatca
     2521 acagcgggag cagcgatcag agcggcttga tcttgatcgg gattgccgtg atcacgatcg
     2581 gattagctgg ctcgatcggc ttcagatcga tcaatcgatc gcgcactcga tcaaaatcaa
     2641 tcaagcgatc gagcaaaccc gatcgatcga tccaactcac ttgtacacac tgcgggcgcg
     2701 tgaatcgagc caccgcaatc tactgcacac agtgcggccg accgatcggt gcgacagtca
     2761 ccgcgacaaa atgcgcgcag tgcgatcatg tcctgcgacc cagagctaag ttctgttcca
     2821 actgcgggaa agcggcctaa ggccagctgt aagaaacatg cggcgcaggt caatgggtaa
     2881 caaatcaggt tggactggcg caagcgcata tgctaatatt taaccaccca aatttctgga
     2941 gccaggtgtg acgctcgatc gacggattga agttttgcga agcgcgttcc ccacaatggg
     3001 cgacgagctg ttggtctacc ttgcgcacaa tgcgcgggaa gtccacacgt cggccggcga
     3061 tcgcatttgc gaagaaggcg aaaccggcga tgcgttctat gtaatcatgg cgggtcgcgc
     3121 gcaggtcagc aaatttatga cgctgggtgc gcagcgcttt ttgaccgaac tgcagccggg
     3181 ccaattcttt ggtgaactgt cgctggtgga cgatgcaccg cgcgtcgcca gcgttgatgc
     3241 gttgaccgag tgcgagctgc tcgtcatcac caaagatgat tttttgaatc tcctgcaaga
     3301 cagtccgtcg atggcggtgc cgatcatgcg ctcagtgacc gctcgtctgc gcgattccga
     3361 tcagcgtacg attaacgaac tacgcgacaa aaacgatcaa ttggctgcag cgtatgccga
     3421 attggaatac acggtcaagc gcaagagcga cttcctgacc aacgtcgcgc acgaactgcg
     3481 cacgccgctc acctcggtca aaggttacgc gcatttgatg cgcaccggca tgttgcacgg
     3541 ccccgacctc gatcgagcgt tcgcagcgat catcgacaac tccgaagcga tcgtgcggct
     3601 ggtgaataac attttgttcc tgcaagagat cgattttatc acgcccgcgc atgatccggt
     3661 ggacatgata cagttgatc
//
LOCUS       DASRTJ010000082         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307187,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000082 DASRTJ010000000
VERSION     DASRTJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307187"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFF70_01560"
     CDS             complement(<1..236)
                     /locus_tag="VFF70_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43408.1"
                     /translation="MTQINLSQPTAISHLRALWAIAKKDWRYYWRYPLNAVSTVLQPI
                     VWLTPVYFMGQAFSVNGEARGFAAYTGSLDYMSF"
     gene            complement(284..1162)
                     /locus_tag="VFF70_01565"
     CDS             complement(284..1162)
                     /locus_tag="VFF70_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43409.1"
                     /translation="MTTSSKVSAMTSLGQSNWLAHWHALAAVIRREWIVFTRYPTWIV
                     ALVIWPVIFPAAYILSARALAGPDGSGLAVFNQSAGTGNYLGFIAIGTTVWMWQNVVL
                     WNVGFALRNDQLHGTLESNWLSPTWRFSFLLGSSVTQLVTMLMFLLISGIEFVLLFGV
                     RFDGNLLLVAIMFVAAIPAIYGLGFAFASLVITIKEANAFVFLVRGIVMIFCGITFPL
                     SVLPAWMQNVAAWLPQTYLIRGVRNAALSGADLPALLPDLGALLLFGIAWLTIGYVTF
                     NWMERRARQTGAIGQY"
     gene            complement(1176..2243)
                     /locus_tag="VFF70_01570"
     CDS             complement(1176..2243)
                     /locus_tag="VFF70_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43410.1"
                     /translation="MFNWAIEAENLVKKFPRRAAPTDRGETANDTNQAAAPAPRRKLF
                     ERKPPLSWSVAVDGVSLQIKQGEVFGLLGPNGAGKSTTIRMLCTLLEPTSGTARVNGF
                     DIVKQGNQVRQSLGTVLAGERSTYWKLTARENLEYFAALYHIPPQTAQRRIDDLLERM
                     ELTSRANDLVEKYSTGMRQRVAIAKALLAQPPIVLLDEPTLGLDPQAARRVRELITEL
                     KTEGHTVLLTTHYMEEADQLSDRIGIIDQGRIIALDTPAVLKRRINQKDAIKLEVAGW
                     HSEMAEALQKLPNVENLVTRHLGTDSLYEVCLHAANSRSILPGIIETLSHNGTRLVNL
                     NIVQPSLEDVFINLTGKALRD"
     gene            complement(2261..2647)
                     /locus_tag="VFF70_01575"
     CDS             complement(2261..2647)
                     /locus_tag="VFF70_01575"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HZY43411.1"
                     /translation="MNQILSQCPVCQHQLEVTKLYCRNCDTTIDGHFEIGNLHQLSAD
                     QLSFLITFVRNEGKINRVGEDLGISYPTVRSRLHDVIRSLGFEVGEEEEIPATMPDEE
                     RQSILESLSQGKLSSEEALKRLSGRS"
BASE COUNT          628 a          724 c          715 g          655 t
ORIGIN      
        1 atgaatgaca tgtaatccag actgccggtg taagccgcga agccgcgtgc ttctccgttg
       61 acgctgaacg cctggcccat gaagtacacc ggcgtcagcc agacgatcgg ctggaggaca
      121 gttgataccg cgttcaacgg atagcgccag tagtagcgcc aatctttttt agcgatcgcc
      181 cacaacgcgc gcagatgtga gattgctgtc ggttgcgata gattgatctg agtcatttga
      241 tcattcttct tctgtcattt tgcatttgct cattattcat tgatcagtat tgtccgatcg
      301 cgccggtctg tcgggcgcgg cgttccatcc aattgaatgt tacgtacccg attgttaacc
      361 aggcgatccc gaagagcagc agtgcgccga gatcgggcag cagtgcgggt aagtccgcgc
      421 ccgatagggc cgcgtttcga acgccgcgga tcagatacgt ttgtggcagc caggcggcga
      481 cattttgcat ccaagccggc aatacactca gcgggaaggt aatgccgcag aagatcatca
      541 cgattccgcg taccaagaag acaaaagcat ttgcttcttt gatggtgatc accaaacttg
      601 caaaggcaaa acctaatcca tagattgccg gaattgccgc gacaaacatg atcgccacga
      661 gcaataaatt gccatcgaag cggacgccga ataagagcac gaactcgatg ccggatatca
      721 ataggaacat tagcatcgtg accaattgcg ttacgctgct gcccagcaaa aaggagaagc
      781 gccacgttgg cgacagccag ttcgattcca aagtgccatg caactgatca tttctcagcg
      841 cgaaaccaac attccacaag accacgttct gccacatcca aaccgttgtg ccgatcgcga
      901 tgaagcccag atagttgcct gttcccgccg attgattgaa taccgccaag ccgctgccat
      961 ccggcccagc aagcgcacgc gccgagagga tgtacgctgc tggaaaaatc accggccaaa
     1021 tgactaaggc gacgatccag gttggatagc gggtgaagac aatccactca cggcggatga
     1081 cagccgctag tgcatgccaa tgtgccaacc aattggattg acctaaagat gtcatcgcag
     1141 aaactttcga ggatgttgtc atgatcgatc gtctttcagt ccctcagcgc ttttccagtc
     1201 aagttgataa acacatcttc gagcgagggt tgtacgatgt tcagattgac cagccgtgtg
     1261 ccattgtggc ttaatgtctc gatgatgccc ggcagaatcg agcggctgtt ggcggcatgc
     1321 aggcaaactt catataacga gtcggtgccc agatggcgcg tgaccagatt ctcaacattc
     1381 ggcagtttct gaagcgcctc agccatttcg gaatgccagc ctgcgacctc taatttgatg
     1441 gcatctttct gattgatgcg ccgcttcagc acggccggcg tatcgagcgc gatgatacgg
     1501 ccttgatcga tgatgccaat tcgatcggat agctgatcag cctcttccat atagtgagtg
     1561 gtcagcagca ccgtgtgccc ttcggtcttg agttcggtga tcaattcgcg tacacgtcgc
     1621 gccgcttgcg gatcgaggcc gagtgtcggt tcgtcgagca ggacgatcgg cggttgggcc
     1681 agtagcgctt tggcgatcgc cacacgctgc ctcatgcccg ttgaatattt ctcgaccaga
     1741 tcattggcgc gggaggtcag ctccatgcgc tctaatagat cgtcgatgcg gcgctgagcg
     1801 gtctgcggcg gaatgtgata caacgccgcg aagtattcaa gattctcgcg cgcggtcaat
     1861 ttccagtacg tgctgcgttc gccggccagc acagttccca aactctgccg cacttgattg
     1921 ccctgtttga caatatcaaa gccattaacg cgtgcggtac cgctcgtcgg ttcgagcaag
     1981 gtacacagca ttcggatcgt cgttgacttg cccgcgccat tcggccccaa taaaccgaat
     2041 acttcgcctt gcttaatttg caggctcacg ccatcaacgg ctactgacca gcttagcggt
     2101 ggtttacgct cgaataattt gcgccgtggg gcaggggcag ccgcttggtt ggtgtcattc
     2161 gctgtttcgc ctctgtcggt cggtgctgcg cgccgaggga atttcttgac taaattctcg
     2221 gcttcaatag cccagttgaa catgatctaa cacctgtcat ttagctgcgg ccactcaagc
     2281 gtttgagtgc ttcttcggat gagagcttgc cctgactcag tgactccaag atagattggc
     2341 gctcttcgtc cggcattgtc gctggtatct cttcttcttc accgacctca aacccaaggc
     2401 tgcgaatgac atcgtgtagt cggctgcgca ccgttggata agagatacct agatcttcgc
     2461 cgacccgatt gatcttgcct tcattccgga caaaggtgat caaaaatgac aattgatcgg
     2521 cgctgagttg gtgcaagttg ccgatttcaa aatgtccgtc gatagttgta tcgcagttac
     2581 ggcagtagag tttagttact tcgagttgat gctggcagac tggacactgg cttaaaattt
     2641 gattcatttg gatacacctt aatctttctg cgaagtatga acagtaaagc agccgaacat
     2701 tgataatgtt aataacagca tc
//
LOCUS       DASRTJ010000083         1720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_342590,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000083 DASRTJ010000000
VERSION     DASRTJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342590"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..944
                     /locus_tag="VFF70_01580"
     CDS             156..944
                     /locus_tag="VFF70_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZY43412.1"
                     /translation="MSTHEHSKHIAIHLDGITVGYNNHPALRDVTLDITRGVRMAVLG
                     PNGAGKSTLFKAIMGLLPLRSGHVLIHGEPPRQRESHVAYVPQREDIDTRFPITAREV
                     VMMGRYGRIGWVRRPAKIDRAAVEQALHQLDIVDLANQSIGELSGGQMQRVFLARALA
                     QEADILLLDEPFTGVDAKTEQAIYKLLDDLKARDVTVLLATHDLDKALSKFDDLLLIN
                     GTVIATGCAEDVFQPELLQRTFGSQVTLMPSGNLVMAATDGHHQ"
     gene            998..>1720
                     /locus_tag="VFF70_01585"
     CDS             998..>1720
                     /locus_tag="VFF70_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZY43413.1"
                     /translation="MFDFLLTPLTYPFMQRALLASILVGGLCAVIGSYVVLRGMAFLG
                     DALAHAILPGVAIAFLLNGDLFLGALITAIVAAIGIGFLTRSGRVREDTAIGIIFSGA
                     LALGVVLISSARSYQTDLTHILFGNVLGVTDRDLMLTVGFGIVVLLIIVLLYKELLVF
                     TFDPTFASTLKLPVDRLRYLLMILLAVTVVVSLQTVGVGLVAAMLVTPAAAASLLTRR
                     LSHMMIVSAIIGIASSIVGVYVS"
BASE COUNT          344 a          473 c          475 g          428 t
ORIGIN      
        1 tgaatatcca gatgaccggg aggcttacac aaacggcaaa agcgcattgg ttgtcgaaat
       61 catgagccga gctcgagttt ggggggcagt tcatagtgat cgttaatccg ggatccggtt
      121 ttgtcatttt tcatttgatc atttgtcatt aacctatgag cactcacgaa cactcgaaac
      181 acattgccat tcatctcgac gggatcaccg ttggctataa caatcatccg gcgctgcgag
      241 atgtcacgct cgatattacg cgcggcgtcc gaatggctgt gcttggcccg aatggcgcgg
      301 gcaagtcaac gctcttcaaa gcgatcatgg gcttgctgcc gcttcgatcg ggtcatgtgt
      361 tgattcacgg cgaaccgccc aggcagcgcg agtcgcatgt cgcttacgtg ccgcagcgcg
      421 aagacatcga tacgcgtttt ccgatcaccg cgcgtgaagt ggtgatgatg ggccgctatg
      481 gacgcatcgg ctgggtccgg cggccggcga agatcgatcg ggcggccgtg gaacaagcgc
      541 tgcaccaact ggatatcgtc gatctggcga accaatcgat cggcgagtta tcaggcgggc
      601 aaatgcagcg cgtttttctg gcgcgcgcgt tggctcaaga agcagacatc ttgttgctcg
      661 atgaaccgtt caccggcgtg gatgccaaga ccgaacaagc catctacaaa ttgctcgacg
      721 atctcaaggc gcgcgatgtg acagtgctgt tagccacaca cgatctcgat aaagcgttat
      781 cgaagttcga tgatctcttg ctgatcaacg gcacggtcat cgccaccggc tgcgcggaag
      841 atgtattcca gcccgaattg ctgcagcgca cttttggatc acaggtcacg ttgatgccca
      901 gcggcaatct ggtcatggcc gcaacggatg gacatcatca gtaacgagta acgagtaaaa
      961 aattacccgc tactcgttac tgattactta accagcaatg tttgacttcc tactgactcc
     1021 attaacctat cccttcatgc agcgtgcact gctcgcgtcg atcttggtcg gcggactgtg
     1081 tgcggtcatt ggatcttacg tggtgctgcg cggcatggcc ttcctcggcg acgcattggc
     1141 gcatgcgatt ctgcccgggg tggcgattgc gttcctcctc aatggcgatc tattcctcgg
     1201 cgcgttgatc acggccatcg ttgcggcgat cggtattggc ttcctcacgc gcagtggccg
     1261 cgtgcgcgaa gataccgcta tcggaattat tttttcaggc gcgttggcgt tgggcgtggt
     1321 cttgatcagc agcgcgcgat cgtatcagac cgatctgacg cacatcttat tcggcaacgt
     1381 cctcggcgta accgatcggg atctcatgct aaccgttgga ttcggaatcg tcgtcttgtt
     1441 gatcatcgtg ctgctttaca aagaactgct cgtcttcacg ttcgatccga cttttgcttc
     1501 aacattaaaa ctgcccgtcg atcgattgcg ttatctgctg atgatcttgc tcgccgtcac
     1561 cgtcgtcgta tccttgcaaa cggtcggtgt gggtttagtc gcggcgatgc tcgtcacacc
     1621 ggcagcggcc gcttcattat taacgcgccg cttatcccac atgatgatcg tttcagccat
     1681 catcggcatc gcctcgtcga tcgtcggcgt gtatgtctcg
//
LOCUS       DASRTJ010000084         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_153336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000084 DASRTJ010000000
VERSION     DASRTJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..528
                     /locus_tag="VFF70_01590"
     CDS             97..528
                     /locus_tag="VFF70_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43414.1"
                     /translation="MPPSKSSGRPASGAPGVKMLITAASLAATVGGWAMLSNTEGVSS
                     STTSTPALPPATTASLTVKLQPLPTLVPPPDPKSIAINAQSVIRSSSNQQVAAAPAQA
                     EPAPQVSGLRIVSAPSVVSGGGGSSVAGAPPPATTTRSSKP"
     gene            535..1458
                     /locus_tag="VFF70_01595"
     CDS             535..1458
                     /locus_tag="VFF70_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZY43415.1"
                     /translation="MQRLEFHAMGCQMLAIVDSDSRLAAKQLAQVPQWFEEWEQHLSR
                     FRESSELNELNRQSGRSIPVSPILWEVTQLAIKAAEWSDGLVSPTILNALEAAGYDRS
                     FEAISPIGTLPTRSADPTPAGQWRAIERRASTRSIQLPSNVRIDLGGVAKGWAAARAA
                     RKLSIHGAALVDASGDIALNGLRLDGQAWPIGITDPLNPDRQLDLIMIDQGGVATSGR
                     DYRRWQHDGQWQHHIIDPRTGLPAQTDVISATVIAPNVYQAEVAAKAAVILGSDQGLS
                     WLEDHPHCAGLLVLEDGRVLFSERMHQYLWR"
     gene            1582..2301
                     /locus_tag="VFF70_01600"
     CDS             1582..2301
                     /locus_tag="VFF70_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43416.1"
                     /translation="MSTKISTPDELDTLPPAMALQSLLLMLLAVAVGAFAAAVVLPNW
                     LPGLGNSLLGEAPKAYWYLSRMSAMAAYGLLWFSMATGLIITNKIARVWPGGPTAFDL
                     HQYSSLLGMAFGLFHALILMGDKYINYTLAQILIPFNSTGYKPIWVGLGQVGFYLLAI
                     VALSFYIRKRLGHKTWRLIHYISFAVFILSLLHGLFSGTDSPLDWARMLYWAGGVSLV
                     FLTVYRILVSVFQPKKQTPAH"
     gene            2480..>2754
                     /locus_tag="VFF70_01605"
     CDS             2480..>2754
                     /locus_tag="VFF70_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY43417.1"
                     /translation="MDTKNQLKIVIAGAGYGGLAAVRSLARNPRLQVTLIDQHEYHLL
                     QFQLHEAAVNKIDVETLAIPLPDLLPRSAKFVQAHITGFDLDQHVVKT"
BASE COUNT          614 a          792 c          713 g          635 t
ORIGIN      
        1 accctcaacc ttgatcgcgg gagccgtgac ccaatcagtc tggccgcgac tcccgttttc
       61 attcattcac tctcacatca tttaggagat acttacatgc caccatcaaa atcatctggt
      121 cgacccgcgt caggcgcgcc cggcgtcaaa atgttgatta ctgcagcgtc actagctgcg
      181 acggtcggcg gttgggctat gctcagcaac actgaaggcg tatcatcatc aaccacatcc
      241 acgccggcct tgccgccggc gacgaccgca tctttaaccg tcaaattaca accgctgcca
      301 acattggtgc caccaccaga tcctaaatcg atcgccatta atgcgcagtc cgtcatccgc
      361 agcagcagca atcaacaagt tgccgcggca ccggctcaag ccgagcctgc accgcaagtc
      421 tcaggtctgc ggatcgtcag cgcaccgtcc gtagttagtg gcggcggcgg cagcagcgtc
      481 gccggcgcac ctccacccgc aaccaccaca cgctcatcaa aaccgtgatc gatcatgcag
      541 cgccttgaat ttcacgccat gggttgtcaa atgttggcca tcgtcgattc ggattcgcgt
      601 ttggccgcca aacagttggc gcaagttccg cagtggttcg aagagtggga gcagcatctc
      661 agccgcttcc gcgaatcgag cgaactcaat gaacttaatc gtcagtccgg ccgatccata
      721 ccagtcagtc cgattttgtg ggaagtcacg cagctcgcga ttaaagccgc cgagtggagc
      781 gatggcttgg tctcgccgac gatcctgaat gcgctcgaag ctgccggtta cgatcgatcg
      841 tttgaagcga tcagtccgat cggcactttg ccaactcgat cggctgatcc aacgcccgcc
      901 ggccagtggc gcgcgatcga acggcgagcc agcacccgat cgatccagct gcccagcaac
      961 gtccgcatcg atctgggcgg cgtggctaaa ggctgggcgg ccgcgcgggc cgcgcgcaaa
     1021 ctatcaattc atggtgcggc cttggtcgac gctagcggcg atatcgccct caacggtctg
     1081 cggctcgacg ggcaagcctg gccgatcggg attaccgatc cgctcaatcc cgatcgtcaa
     1141 ctcgatctaa tcatgatcga tcaaggcggc gtggctacgt cagggcgaga ttatcgacgc
     1201 tggcaacatg atggacagtg gcagcatcac atcatcgatc cacgcaccgg cctgcctgca
     1261 cagaccgatg tgatcagcgc caccgtcatc gcacctaacg tgtatcaagc cgaagttgcg
     1321 gcaaaagcag ctgtgatctt gggcagtgac cagggcttga gttggctgga agatcatccg
     1381 cactgcgccg gcttactcgt acttgaggat ggtcgcgtac tctttagcga acgaatgcat
     1441 caatatctgt ggcgataata agttaacaca gccattcccg ccggacactt tatcaggcgc
     1501 ggaaatccag aaacgatcac taaacacccg cttgcgcggg tgtgccaatc gataactgaa
     1561 ttattttcga atagaggaat catgtctaca aaaatttcta cgcccgatga actcgatact
     1621 ttgccgccag cgatggcttt gcaaagtttg ttgctcatgc tgctggcagt ggccgtgggc
     1681 gcctttgcgg ccgcagtggt tctgcctaat tggctgcccg gcttgggcaa ctctttgctc
     1741 ggtgaagcgc ctaaagcgta ttggtatctt tcgcgcatga gcgccatggc cgcctatggt
     1801 ctgctgtggt tctcgatggc gaccggcttg atcatcacca acaagatcgc gcgcgtttgg
     1861 ccgggcggcc cgaccgcctt cgacctccat caatactcaa gcctattggg catggccttc
     1921 ggcctgttcc atgctttgat cttgatgggt gacaaatata tcaactatac gcttgcccag
     1981 atccttattc cattcaacag tactggctac aaaccgatct gggtcggtct gggtcaagtc
     2041 ggcttttatt tactcgcgat cgtcgcgctg agtttctata tacgcaagcg gctcggccac
     2101 aaaacctggc gcttgattca ctacatcagc tttgcggttt tcatcttgtc gctattgcat
     2161 ggcttgttca gcggcacaga cagccccttg gattgggctc gcatgcttta ctgggcaggt
     2221 ggagtcagtc tagtgtttct gacggtctat cgcattctgg tgtcggtttt ccagccaaag
     2281 aaacagacac ctgcgcatta aagccattcc tgccgaattt cccagccccg attttaggta
     2341 aaatctgggc ttttttattg ctgaaattga tagttgacat tctcacaaaa ttgtgatata
     2401 aatcactata taaagatgat agatgtcatc tttatcagaa aaactagacc aacgggctta
     2461 aaggccgctg gaggcgacaa tggataccaa gaatcaactc aaaattgtaa ttgcaggcgc
     2521 aggttatggc ggcttggccg cggtgcgaag tttggcacgg aatccccgct tgcaagttac
     2581 cctaatcgat cagcacgaat atcatctatt gcaatttcaa ctgcatgaag cggccgtcaa
     2641 caagatcgat gttgagacgc tggcaatccc tctgcccgat ttgctgccgc gctcggcgaa
     2701 atttgtgcag gcgcacatca caggtttcga tctcgatcag catgtggtca agac
//
LOCUS       DASRTJ010000085         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_4764,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000085 DASRTJ010000000
VERSION     DASRTJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4764"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFF70_01610"
     CDS             <1..760
                     /locus_tag="VFF70_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZY43418.1"
                     /translation="VIFGDRDLVKLKGEDLRHFRREMQIIFQDPYASLNPRMTVGEII
                     GEPLEIHGTTKGKDKEDRVKALLKEVGLNPSFSTRFPHEFSGGQRQRIGIARAIALNP
                     SFIVCDEPIAALDVSIQAQVVNLLEELQAKYGLTYLFIAHDLSMVRHISDRVAVMYLG
                     RIVEIADWRMIYDDPKHPYTQALLSAIPIPDPVLEAKRQRTILEGDVPSAANPPVGCN
                     FNTRCPVRQERCFHDDPELRELAPGHWVACHFAV"
     gene            complement(960..1223)
                     /locus_tag="VFF70_01615"
     CDS             complement(960..1223)
                     /locus_tag="VFF70_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43419.1"
                     /translation="MKLVGSFEPLGPAVGTTTGGVVGAATTGALVGATVAAGVAAGAQ
                     ALTTMLKTASILTINQIFLVRNILFLLLENVSVKSSIQYDAGW"
     gene            1291..3048
                     /locus_tag="VFF70_01620"
     CDS             1291..3048
                     /locus_tag="VFF70_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY43420.1"
                     /translation="MLFNIMEGLVTFDGADPLKVKPQLAKAIPDPVASADGKGVSYTW
                     NIVDGVKFHNGDPMMAHDVAFSFWRTMLVGDNAVSPAFLVLEAFFLDSAGNPVDDPTQ
                     LVDESGALISAPDDLKAAKPADLEAACKKVQDAVTFDDAAGTVTMNMDHKWGPLMVTL
                     AGGAWAFVSDQAWVKAQGDWDGDCKTWQNFYSIPSESGVLRDKTNGTGPYVLDHWTPN
                     EELVMTANPNYRKGPAKIGRIVLKIVNEFGTRFATYQAGDADAITLGSTADRPQMDTL
                     VKDECDLTTGACQPYSPANPNGILRRYLKLPSVSRTDISMNYLVADGSPFIGSGLLDG
                     AGVPADFFSDINIRKAFAQCFDIDTYVKDVFLGEGTPGLALSLPGQPGNETATRYAFD
                     LDKCKASFEASTLKNADGKGVMDVGFYLQFGYNTGNTSRQTIAQILASSLAKVNPNFL
                     MVPVALPWPTFLRAQSAKQLTISTAGWQEDIHDPHNWYVPYLLGTYASRANVPAELKT
                     QFQTLINQGVFETDQAKRATIYAQLNQLVYDNAVFILGATGDGRKYEPLYVHGWWEGV
                     NRNPMINGQGIYVYELSKD"
     gene            3248..4324
                     /locus_tag="VFF70_01625"
     CDS             3248..4324
                     /locus_tag="VFF70_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43421.1"
                     /translation="MYAYIIRRLLIIPVLLFGVTILLFLMISLLKPDERLALYIKDTP
                     RNEAVVKGLIKRYGLDQPIYVQYANWLFGRQDEDPVTHVVTVKGGILRGDFGWSKTGT
                     DTIARVIQRRFPATVELALWAIIPIVGVGIWMGVLAAVNHNKLIDQILRVFAIVGYSF
                     PSFVFGLLVLMFFYARLQWFPPGRLSDWASQVVQTPAFVHYTSMNTIDGLLNGRLDIF
                     LDALRHLVLPILTLAYIQWALLLKVTRSSMLESLRQDYVTTARAKGLSERNVINRHAR
                     PNAMIPVITVSGFTIVGLLNGVIITETIFVYPGIGSIAAQAAAQLDVLTVLGFTLFNG
                     FILLMANLVVDVLYAVIDPRVRLS"
     gene            4365..>5194
                     /locus_tag="VFF70_01630"
     CDS             4365..>5194
                     /locus_tag="VFF70_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43422.1"
                     /translation="MASVDIAVLQPLDADNLPASRKPPSIGKQLISNRLSVSGFILLT
                     FFALIAIFAPVLAPPLPNTRNPMFIPRDGFNLVPQPPGTAWDRNPPPLPFWWQAVTGL
                     DHWTHPFGMASGGWDIYYGVIWGTRTAFIAGIVITLVTLVIGVLVGSFSAYYGGVIDL
                     VTQRITEVFMTFPFLMAALTAATILTPLLGAKAGVGDAGLVTAGVAIIIFGWMGYARL
                     IRGDILSVRERDYVMAARAVGAKDFRIMIKHIIPNAIFPTLVVASLDIGTYVVTFAAL
                     S"
BASE COUNT         1061 a         1436 c         1480 g         1217 t
ORIGIN      
        1 ggtgatcttt ggcgatcggg atttggtgaa actgaaaggt gaagacctcc gccatttccg
       61 ccgcgaaatg caaatcatct ttcaagatcc gtatgcttct ttgaatccgc gcatgaccgt
      121 gggcgagatc atcggtgagc cgctggaaat tcacgggaca accaaaggca aagacaaaga
      181 agaccgggtc aaggcgttgc tgaaagaagt cggcttgaat ccttcattca gtacgcgttt
      241 cccgcacgag ttctcaggcg gccagcgtca acgcatcggt attgcgcggg ccattgcctt
      301 gaatccatcc ttcatcgtgt gcgatgagcc gatcgccgca ttggacgttt cgattcaagc
      361 gcaagtcgtg aatctgttgg aagagctgca agcgaaatac ggcttaacgt atttgtttat
      421 cgcgcacgac ttgtcgatgg tgcgccacat cagcgatcga gtggccgtga tgtatctggg
      481 acggatcgtg gagatcgccg actggcgaat gatctacgat gatcctaaac acccttacac
      541 tcaagccttg ttgtcggcga ttcccattcc cgatccggta cttgaagcga agcggcagcg
      601 gactattttg gaaggcgatg tgcccagtgc ggcaaatcca ccggtgggct gtaatttcaa
      661 tacgcgctgt ccggttcgtc aagaacgctg cttccatgat gatccggaac tgcgtgaatt
      721 ggcgccggga cattgggtag cctgtcactt cgccgtttag tttgaccgta tctgctcagg
      781 cgtcattgcg agaagcgtcc tgagcaaagc ggtagctctt tgccgcgcag tcgaaggatg
      841 caacgaaaca ctctctgcat cgatcgggag attgcttcgc aaaaaacgct cgcaatgacg
      901 gttgaaatct gagaggaggt gaggacctga acgaaaatta aagcaattcg ccagccggat
      961 caccagccgg catcgtactg aatcgaagat ttaacactca cattctctag gaggagaaac
     1021 aaaatgtttc gcaccaagaa gatttggtta atggtaagta tgctcgcagt tttaagcatg
     1081 gtcgtcagcg cctgcgcgcc ggctgccact cccgcggcaa ctgttgcgcc gactaaggcg
     1141 ccggtagttg cagcaccgac gacgccgcct gttgttgtgc cgaccgcagg ccccaagggc
     1201 tcgaaggatc cgaccagctt cacggaattg accttcggcg atccggaaac acttgacccg
     1261 gcggtcgact atgagacagc cggctcggcc gtgctgttta acatcatgga aggcctggtc
     1321 acgttcgacg gtgctgatcc gttgaaagtt aagccccaat tggctaaggc catccccgat
     1381 ccagtagcca gcgccgatgg caagggcgtc agctacactt ggaacatcgt cgatggtgta
     1441 aagttccata atggcgatcc gatgatggcg catgacgtgg ccttctcgtt ctggcgcacc
     1501 atgctcgtcg gcgacaacgc cgtatccccg gccttcctgg tcctggaagc cttcttcctt
     1561 gatagcgccg gcaatccggt cgatgacccg acccaattgg tcgatgaatc gggcgcgttg
     1621 atcagcgctc cggacgatct aaaggccgcg aagcccgcgg acttggaagc ggcctgcaag
     1681 aaggtacagg atgcagtgac gtttgacgat gccgccggta ccgtgacgat gaatatggat
     1741 cacaagtggg gtccgctcat ggttacgtta gcgggtggtg cctgggcctt cgtgtcggat
     1801 caggcttggg ttaaggctca aggtgattgg gatggcgact gcaagacctg gcagaacttc
     1861 tacagtattc cgtcagagtc tggcgtgctg cgcgacaaga ccaacggcac cggtccctac
     1921 gtgctcgatc actggacccc caacgaagag ttggtcatga cggccaaccc gaactaccgc
     1981 aagggccccg ccaagatcgg ccgtattgtg ctcaagatcg tgaatgaatt cggcacgcgt
     2041 ttcgccacgt atcaagcggg cgatgctgat gcgatcactc ttggctcaac cgccgatcga
     2101 ccgcagatgg atacgctggt caaggatgag tgcgacctca cgactggcgc gtgccagccg
     2161 tactcgccgg ccaacccgaa cggtatcctg cgccgctatc tcaagctgcc gtccgtgagt
     2221 cgcacggata tcagcatgaa ctatctggta gcagacggca gcccgttcat tggcagcggc
     2281 ttgttggatg gcgccggtgt gccagcggat ttcttcagtg acatcaacat ccgcaaggca
     2341 ttcgcccaat gcttcgatat cgatacctac gtcaaggatg tgttcctcgg cgaaggcaca
     2401 cccgggttag cgttgtcgct gcccggtcag cctggcaacg aaaccgccac gcgctatgcg
     2461 tttgatctgg ataaatgcaa agcgtcattc gaggcctcca cgttgaagaa cgccgatggc
     2521 aaaggcgtca tggatgtcgg tttctacctg caatttgggt ataacaccgg caacacttcg
     2581 cgtcagacga ttgcccagat tttggcgtcc agcctggcga aggtgaaccc caacttcctg
     2641 atggtgcccg tggcactgcc gtggccgacc ttccttcgcg cccagagcgc taagcagctg
     2701 acgatcagca ccgccggctg gcaggaagac attcacgacc cgcacaactg gtacgttccg
     2761 tatctgctcg gcacgtacgc gtcacgcgcg aatgtgcccg cagagttgaa gacccagttc
     2821 cagactctga tcaatcaggg cgtgtttgag accgatcagg cgaagcgtgc gaccatctac
     2881 gcgcagctca accagctggt ctatgacaat gccgtgttca tcctcggtgc taccggggat
     2941 ggccgcaagt acgagcctct ctacgtgcac ggctggtggg aaggcgtgaa ccgcaacccg
     3001 atgatcaacg gtcaaggcat ctacgtttac gaactgagca aggactagtt tttcgcagtc
     3061 caaactcgac gcgttaggtt aagaatggca ggggccgagc aatcggcctc tgccaacctg
     3121 atcagatgac tggttgatcc ataagccgct gggcttgtgc atcgaccagt gcgcagcgaa
     3181 tgtgaaccaa tgctgcgctt cgttggtaag tacagtaagt gccaagatgg agtaggagag
     3241 taagcccatg tatgcttata tcattcgacg cttattgatc attcccgtcc tcttgtttgg
     3301 cgtgacgatc ttgctcttcc tcatgatcag tctgttgaag ccggacgagc gcctggcttt
     3361 gtatatcaaa gacaccccgc gcaatgaggc ggtggtcaag gggttgatca aacgctacgg
     3421 gctggatcaa ccgatctacg ttcaatatgc caactggcta tttggccgtc aagatgaaga
     3481 tcctgttacg catgttgtaa cggttaaagg cggcatcctg cgcggcgatt ttggctggtc
     3541 gaaaacgggc accgatacaa ttgcccgcgt gattcaacgc cgcttcccgg ccaccgtcga
     3601 gttggcgttg tgggcgatta tacccatcgt aggggttggc atctggatgg gtgtcctggc
     3661 cgccgtcaat cacaacaaat tgatcgatca gattttgcgc gtcttcgcca tcgttggcta
     3721 ttcgttcccg tccttcgtct tcggcttgtt ggtgttgatg ttcttttatg ctcgcttgca
     3781 gtggttcccg ccggggcgct tatccgactg ggcttctcaa gtggtgcaga cccccgcctt
     3841 tgtgcattac accagcatga atacgatcga cggtctgctg aacggccgcc tggatatttt
     3901 tctggatgcc ctgcggcatt tagtcctgcc gatcttgacg ctggcttaca tccagtgggc
     3961 gctcttgttg aaagtgaccc gttcgtcgat gctggaatcg ctgcgacaag attatgtgac
     4021 caccgcgcgc gccaaaggcc tgagcgagcg caacgtcatc aaccggcatg cgcggccgaa
     4081 tgcgatgatc ccggtgatta ccgtcagcgg ctttacgatc gtcggcttgc tgaacggcgt
     4141 gatcattacc gaaacgatct ttgtctatcc gggcatcggc tcgatcgccg cgcaggctgc
     4201 ggctcagctg gatgtgctga ccgtattggg cttcacgcta tttaacggtt tcatcttact
     4261 catggccaac ctggtggtcg atgtgttgta cgccgtcatc gatccgcgag tgcgcctgag
     4321 ctgatgtttg aatttccaag ctccgtctat cgggagaaaa gataatggcc agtgtggata
     4381 ttgcagtttt gcaaccttta gatgcagata atttgcccgc ttcacgcaag ccgccgagca
     4441 ttggcaagca gctgatctcg aaccggttat cggtgtcagg cttcatcttg ctaaccttct
     4501 tcgcgctaat cgcgatcttt gcgccggtgc tggcgccgcc gctgccaaac acgcgcaacc
     4561 ccatgttcat tccacgcgat ggcttcaatc tggttccgca gccgcccggt accgcttggg
     4621 atcgtaatcc tcctccgctg ccgttctggt ggcaggccgt gaccggttta gatcattgga
     4681 cacacccctt cggcatggcg agcggcggct gggacattta ctatggtgtg atctggggca
     4741 cgcgcaccgc gttcatcgcc ggcattgtca tcacactggt taccttggtg atcggcgtct
     4801 tggttgggtc gttctcggca tattacggtg gtgtcatcga tctggtcacg cagcgcatca
     4861 ctgaagtgtt catgaccttt ccgttcctga tggctgcttt gactgccgcc acgatcttga
     4921 cccctttgct aggggctaaa gctggcgtcg gcgacgcggg cttggtcacc gctggtgttg
     4981 ctattattat attcggttgg atgggctatg cgcgcctgat ccgcggtgac attctatcgg
     5041 tgcgggagcg tgactatgtc atggccgctc gtgccgtcgg cgcgaaagat tttcgcatca
     5101 tgatcaagca catcatccct aacgcgatct ttcccacgtt ggttgtggct tctttggaca
     5161 ttggcacgta cgtcgtcacc tttgcggcgc tgtc
//
LOCUS       DASRTJ010000086         8437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_4771,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000086 DASRTJ010000000
VERSION     DASRTJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4771"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFF70_01635"
     CDS             complement(<1..208)
                     /locus_tag="VFF70_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZY43423.1"
                     /translation="MNDSFVEVPSGPFIYGPEECYERLEFCPPLKPQLTIDLPEFWIA
                     TSPVTYLEWRSFLEATGYKWSGQWY"
     gene            complement(201..2039)
                     /locus_tag="VFF70_01640"
     CDS             complement(201..2039)
                     /locus_tag="VFF70_01640"
                     /inference="COORDINATES: protein motif:HMM:NF039831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HZY43424.1"
                     /translation="MSLSLIGGAAATRTLIKRHRSEQDNMRAAICVDFDDAYSAAIRA
                     GMPFDDLLHQLKQHGATHVSLPELSLNRLIHSGQLGTRAAVRPRTTKPKVGHWNYLYG
                     APDLIEHLVDELSVRLPYTEASTIGKSTLVFAGELPSIGEIGLGFDFDLASSIIKHGL
                     GIVPRPISYAWPENYLIDRTLTQAAALGKLIAFDGNLILGHEMHLAETLEVMERENLW
                     FAYFAEARHQKGDWFIAKRRAPNVILAHHFTPEEMIPLDFHAAAHQWGHLARERGIRM
                     CYVNFFKVLHATDPLDGLHYIEHIKEELEENGFEVTADVMQSSDSSSTATEAIATKQD
                     LALTGLASAGIASAAINSLFDLPEPIALMTTAVAASGALMLPSLERARGHLEEQYAPS
                     YAPKLIALSAASLAPVAAQQLMRQDRSAGIISGVLLNIAGAAAMAAATSDRDYRLRIE
                     DYRGFNLDWWLPLASAALSIPNKSLRIGILASLSGAWFMLNQRRIDPLALIDPAHAEG
                     HTHHLSTFARTIGDVKIAIGPRPARKWAAAGPIGLALALALADRGSRNLSALAGTVGS
                     IGYVLGLTGFRRSERSILITAKEAGRSLAIGTALSFLIPALQRNNE"
     gene            complement(2101..3258)
                     /locus_tag="VFF70_01645"
     CDS             complement(2101..3258)
                     /locus_tag="VFF70_01645"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZY43425.1"
                     /translation="MTALQSTQLILDVLRPNQTPTRWESDRIRANADWDDLAVRSIVL
                     GLAPQLYRRFSAWGNEIPARAAAKFTATYQATANRNTGIYQQLDEILRACDQCSLEPI
                     ALKGIHLAAKLYPDRALRPMNDIDLLFKPDELPAAEDLLESIGYGGKHKSAELGAGVT
                     KHTSTFKRVSDQAATTNPYLSNASGCIIEPHVSLEESWFGLKVDITPGVRERSIEVDL
                     NGQPGRVLAPEDLLLHLCVHFCFHLVMGAPSMVQLSDLLIMTQVDQLDWPRLIDRSNA
                     TRSIPFVLAALSLAQKLLGAPVPDDAIDQLAQLTSPRQRQRIQQLGLIDVLRRTQQKP
                     LTSIGQRIQRGFHDRAETARWAQDWRGRWQVWQTLLKVFKTDTGRMILRKG"
     gene            complement(3669..3797)
                     /locus_tag="VFF70_01650"
     CDS             complement(3669..3797)
                     /locus_tag="VFF70_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43426.1"
                     /translation="MPKTKRPSSKSDGGPLGFEEKLWAAADKLRGHMDAAELFNAQ"
     gene            4189..5400
                     /locus_tag="VFF70_01655"
     CDS             4189..5400
                     /locus_tag="VFF70_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43427.1"
                     /translation="MRAELDQAHAQLEARVTERTRELTSAFELSQEIIAQIDLETLLR
                     SATDRARALTHADAASLCLIDQDSRTLILSAGSGSGALPMGLRQPIGFGLYPADQVID
                     QGLSIATESACTNCGFLHARTPGQCAAVPLRVGDRTLGALCVVRDHGPVFDSDETRAL
                     TLLANSAAIAIVNARLIEAGRRQAEQAAAVAEREYLAAELHDNIAQTLGFLKLKIDQV
                     KGLLDRGAIDSAQLELDRMQRTTGEAFQQVRAALTGLREPLPSAENLAEKISACLADF
                     RQATGMPAELIIDDQSALTLPHVMQTQALLIVREALNNVRRHAQATQAWVRIDRVDGE
                     ARFSVEDNGRGFVSASVDTENHLGVSIMRTRAERSHGYLTIDSAPGAGTKVTAHFSLA
                     VPSDIAQAVRV"
     gene            5397..6068
                     /locus_tag="VFF70_01660"
     CDS             5397..6068
                     /locus_tag="VFF70_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43428.1"
                     /translation="MTAARILLADDHDLFRAGLAGLIEAQPDLEIVGQAEDGLEALSL
                     VRVLKPDLIVMDIKMPMCDGLEATRLIRTEMPDARIVILTVHDEDEKLFDAIKAGASG
                     YILKDTNSTDFLAALRRALAGDAVLPPKLAARVLDEFARLAQQAAPAALPIVTPDLTT
                     REREVLNLIATGATDKEIAAQLSLSLHTVKSHVHSILSKLHAINRRHAADLAGRHIHP
                     KTIKR"
     gene            6255..6539
                     /locus_tag="VFF70_01665"
     CDS             6255..6539
                     /locus_tag="VFF70_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43429.1"
                     /translation="MLSREASVRIIGSAPSPNAALPMLSACQTDAVVVAGENEMSTAA
                     FGQLLATYPDLPVICADLSRDSVQVITSHCIGTRAADLLAAVAELPKRRS"
     gene            6540..7583
                     /locus_tag="VFF70_01670"
     CDS             6540..7583
                     /locus_tag="VFF70_01670"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY43430.1"
                     /translation="MNFAPAQPIEYTEILYSKADWIARITLNRPQNYNAYSTNTLIEL
                     AAAFRQAAFDDEVAVIVYTGAGDRAFCTGGDVKEYESTYTKSPHDYWKYMGLFSAYIE
                     SILNSGKPVIARLNGLAVGGGNESHLACDLSVIAEHAYVGQVGTSVGSVAAGGATQWL
                     PIFVGDRRARWMVMLNRKISAYQALDWGLVNEVVPSIKKDGAFIDHATPEQIAKASKG
                     QDGYSIDLARLDESVNALCAELIDKFPECMRYTKQQTNFWKDLAWHQTVGHARDWLSI
                     HFASWEPVEGMGAFVEKRAPGYRMLRERAANGQSSEFPWGPYANSCPTCGSKYLPSDF
                     EFCGKCGSKLNGA"
     gene            7611..>8437
                     /locus_tag="VFF70_01675"
     CDS             7611..>8437
                     /locus_tag="VFF70_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HZY43431.1"
                     /translation="MAIRVLDSKPYGTLQEALYLCRDLLEDTDFPTVKRWREAGGKVV
                     GHFQVYFPEEIVHAAGMLPFKVRGAPIEARQAEARFGSYLCSIIKTSLELVLSQRVEL
                     DMFVTHPICDAARNLGAVWGRNFSYPCQILYLPQNANSAHAAEYLRGEYDRLKRDIEA
                     IAGHSITDDALRASTILFNENRRLLRHLYAIKRETPWLLSAAEAYMLTAVGGLIPREE
                     HNELLKAVIPQIESRSAKPQDRIRVVFEGGFCEQPPLDLLHNISQSIYVVDDDLLIGL
                     "
BASE COUNT         1864 a         2336 c         2352 g         1885 t
ORIGIN      
        1 gataccactg cccgctccac ttgtagccag tcgcttctaa aaaacttcgc cactccaaat
       61 aagtgactgg cgaggttgcg atccaaaatt ccggcagatc gatcgttagc tgcggcttga
      121 gcggcgggca aaattctaat cgctcgtaac attcttcagg gccgtaaata aatgggcctg
      181 atggaacttc gacaaatgaa tcattcattg ttgcgctgca acgctggtat caagaaactt
      241 agtgccgtgc cgatcgctaa actcctgccc gcctctttcg cggtgattaa gatcgatcgt
      301 tccgatcggc gaaagccggt cagtcccaac acatagccga tcgatccaac cgtgcccgcc
      361 agtgccgata aattccggct gcctcgatcg gccagtgcga gggccaacgc taagccgatc
      421 gggcctgcgg ctgcccattt tcgcgccggg cgtggtccga tcgctatctt cacgtcgccg
      481 atcgtgcgcg caaatgtcga gagatgatgc gtgtgccctt cggcatgcgc cggatcaatt
      541 agcgccaacg gatcgatccg gcgttgattc aacatgaacc aggcaccact taagcttgcc
      601 agaattccaa tacgcagaga tttgtttggg atcgataaag ccgccgaagc taatggcagc
      661 caccaatcta aattgaagcc gcgataatct tcaatccgca gccgataatc acgatcgctc
      721 gtcgcagcag ccatcgcagc cgcaccggcg atattcagca atacaccaga gattatcccc
      781 gccgatcgat cctgtctcat taattgttgc gcggccaccg gcgcgagtga agcggcactc
      841 aatgcgatca acttcggcgc atacgatggc gcatattgtt cttccaaatg tccgcgtgct
      901 cgttcgagag agggcagcat cagcgcgccg cttgccgcaa cagccgtcgt cattaaggca
      961 atcggttcag gcagatcaaa cagtgaattg atcgccgccg aagcaatacc ggccgaggcc
     1021 aaacccgtta acgccaaatc ttgtttcgtg gcgatcgcct cagtagcagt tgaactgcta
     1081 tccgaacttt gcatcacgtc ggctgtcact tcaaagccgt tttcttctaa ctcttccttg
     1141 atatgctcga tgtaatgtag gccatccaat ggatcggtgg catgcagcac tttgaaaaaa
     1201 tttacgtagc acatgcgaat gccgcgctca cgcgccagat gtccccattg atgcgctgcg
     1261 gcatgaaaat cgagcgggat catttcttcg ggcgtgaagt gatgcgctaa gatcacattc
     1321 ggcgcgcgcc gcttcgcaat aaaccagtcg cctttttgat gacgcgcttc cgcgaaataa
     1381 gcgaaccaca aattttcgcg ttccatcact tccaacgttt cggccagatg catctcgtgc
     1441 cccaagatca gattgccatc aaacgcgatg agtttaccca gagccgcagc ttgagtcagt
     1501 gtgcgatcga tcaaatagtt ttcgggccag gcataactga tcggacgcgg cacaatcccc
     1561 aagccgtgct tgatgatgct gctggctaga tcaaaatcga aacccagacc gatctcgccg
     1621 atcgatggca attcgcccgc gaagaccaag gtggattttc cgatcgtgct cgcttcggtg
     1681 tacggcaggc gaaccgacag ctcgtccacc agatgctcga tcaaatccgg cgcgccatac
     1741 agataattcc aatgaccgac ttttggtttc gttgtgcgtg gtcgaacggc tgcgcgcgtc
     1801 cccagctggc ctgaatggat caatcgattc aacgagagtt cgggcagcga gacatgagtc
     1861 gcgccgtgtt gcttcaattg gtgcagcaga tcgtcgaagg gcattccggc gcgaatggct
     1921 gcggagtaag catcatcaaa gtcgacgcag atggcagcgc gcatgttgtc ttgttccgat
     1981 cgatgccgtt tgatcaacgt gcgcgtagct gctgcgccac cgatcaggga taaagacacc
     2041 gacagaagct gcgataattt catgcgctga taattggaga gacgacccgc cgggtcgtct
     2101 ctaccctttc cttaaaatca tgcggcccgt atcggttttg aaaactttca gcagggtctg
     2161 ccacacttgc caccgtccgc gccagtcttg cgcccagcgc gcagtttcgg cgcgatcgtg
     2221 aaagccgcgt tggatacgct gcccgatcga ggtcagcggc ttttgctgcg tgcggcgcag
     2281 aacatcgatc aggccgagct gctgaatgcg ttggcgctgt cgaggcgagg tgagttgtgc
     2341 aagttgatcg atcgcgtcat ccggcaccgg cgcgcctaaa agtttttgcg ccagcgataa
     2401 ggccgccaag acaaacggaa tcgatcgggt tgcattcgat cgatcgatta accgcggcca
     2461 atcgagttga tcgacttgcg tcatgatcaa gagatcgctt agttgaacca tcgagggcgc
     2521 acccatgacg agatggaaac aaaagtgcac gcaaagatga agcagcaaat cttccggcgc
     2581 gaggacgcgg cctggctgac cgttaagatc aacttcgatc gatcgttcgc gcacgccggg
     2641 cgtgatgtct actttcaaac cgaaccacga ttcttccagc gagacatgcg gttcgatgat
     2701 gcagcctgat gcgttcgata aatagggatt ggttgtcgca gcttgatcgc tgacacgctt
     2761 gaaagtgctg gtatgtttgg taacgcctgc gccgagttca gcggatttgt gtttaccgcc
     2821 gtagccgatc gattccagca gatcttcagc agcaggtaac tcatcaggct taaacaacag
     2881 atcgatatca ttcatcggcc gcagagctcg atcgggatac agcttggccg cgagatgaat
     2941 ccctttcaag gcaatcggct ccaagctgca ctgatcacac gcgcgcagaa tttcatcgag
     3001 ctgttgatag atgccggtgt tgcgattggc ggtggcttga tatgttgcag tgaatttcgc
     3061 ggctgctcgc gccggaattt cattgcccca cgcgctgaag cgtcgataca gctgcggggc
     3121 caggcctaac acgatcgagc gcacggccag atcgtcccag tctgcgttcg cgcgaatacg
     3181 atcagactcc cagcgagtgg gagtctgatt ggggcgcagc acatctaaaa tgagttgagt
     3241 cgattgaagt gcggtcatgt catagccaca gagagacgcg ctacgtaaaa cgaaccggca
     3301 gctgccgggt gatcatctag tcttactggt aaaactcagc cccacgttta gtggctgctg
     3361 aaagtgacct gagacaggtc gtcgtgcttg gtgatctctg gcttggtata catgagtcgc
     3421 ctcctaggag ttaacttgct tgaacgggcc atcgggcgta gcgcgcctct ctctgactac
     3481 cacactcgat tggtaaagta taattcgaaa aagtgtatgg ggcaagctat tgaacaatac
     3541 gaataatccg ggtcggcgga tgtccggagg agcgaaatca gcatcgatct gcggcccgtt
     3601 gattgatgtg tcggctgctt gagtttcttc ggaataagtc agggtcatcg aaacaatttt
     3661 gacagtggtt actgtgcgtt gaataattcg gcggcatcca tgtggccgcg cagcttatcg
     3721 gcggcagccc acagtttttc ctcgaagccg agcggcccgc cgtcgctttt agatgaaggg
     3781 cgttttgttt taggcatggt gtaaagggat tatagcatcg acatcgatcg aatgtgggat
     3841 gaaaatgaga ttgccacggc gcgaaccctg caaacagcag gcagggcagg cagatgcgcc
     3901 tcgcaataac gatgccttcg ccggattaac attcgaggcg gaatcttttc gtatgcctcc
     3961 ccttccttcg actgcgcggc gacaaatcac gggtcgatgt ggattacagc ctgtgcgtgc
     4021 acacccgtct tgttcctagt tgacgaatcc ttgcgagtgt gctaacttcc atagcattga
     4081 cggatagatc gaacgtaagc cgatacgtgg tgacgctgcc tgcatgactt gggaggattc
     4141 ggattgagcg acgctgagct tgcccaactg tctcgatcga ttgagatcat gcgtgccgaa
     4201 cttgatcagg cacatgctca actggaggcg cgcgtcacgg aacggacccg cgaactcacc
     4261 tcggcgtttg aactgagcca agagatcatc gcgcagatcg atctcgaaac cttactgcga
     4321 tcagccaccg atcgagcacg cgcgctgacc catgccgacg cggcttcact ctgcttgatc
     4381 gatcaagaca gccgtacgct gatcctttcg gcgggcagcg gcagtggtgc gctcccgatg
     4441 ggtttacgcc agccgatcgg ctttgggctg tacccggctg atcaagtgat cgatcaagga
     4501 ctctcgattg ctactgagtc agcctgcacc aattgtggtt tcttgcatgc acgcacgccg
     4561 ggccaatgcg cggctgtgcc gttgcgtgtt ggcgatcgca cactcggcgc gttgtgtgtc
     4621 gtgcgcgatc acggaccagt ctttgattct gacgagacgc gcgcgctgac tctgctggca
     4681 aactcggcgg cgattgccat tgtcaatgcg cggttgattg aagctggacg tcggcaggcg
     4741 gagcaggcgg ccgcagtggc agaacgggaa tatctcgccg ctgaactgca tgataatatc
     4801 gcgcagacat tgggtttcct taaactcaag atcgatcaag tgaaaggcct gcttgatcga
     4861 ggcgcaatcg attcggcgca gctggaactc gatcgaatgc agcgcacgac gggcgaggct
     4921 ttccagcaag tgcgcgcggc attaacgggt ttgcgcgagc cgttaccatc cgccgaaaat
     4981 ttagcggaga aaatttcggc gtgcctcgct gattttagac aggcgaccgg catgcccgcc
     5041 gagttgatca ttgatgatca atctgccttg actctgccgc atgtgatgca gactcaggcc
     5101 ctactgattg tgcgcgaagc gcttaataat gtgcgacgcc atgcgcaggc cactcaggct
     5161 tgggtgcgca tcgatcgggt tgatggcgaa gcgcgcttca gtgttgaaga caacgggcgt
     5221 ggctttgttt cggcttctgt cgataccgaa aatcatttgg gggtatcgat catgcgcacc
     5281 cgtgccgagc ggagtcacgg ctatctcacg atcgattcag cgcccggtgc aggtactaaa
     5341 gtcacagcac acttctcgtt agctgttccg tcagatattg cacaggcagt ccgcgtatga
     5401 ctgccgctcg aattctactg gccgacgatc acgatctgtt ccgtgcagga ctggccgggc
     5461 tgatcgaagc ccaaccggat ctcgaaattg tcggacaagc agaagacggc ttggaagcgc
     5521 tctccctcgt gcgtgtgttg aaacccgatc tcatcgtcat ggatattaaa atgccgatgt
     5581 gcgatggact cgaggccacc cgtttgatcc gcacggagat gccggatgcg cggatcgtca
     5641 tcctcaccgt gcatgatgaa gacgaaaaac ttttcgatgc aatcaaagcc ggggccagcg
     5701 gttacattct taaagacacc aactcaaccg attttctggc cgcgttgcgt cgcgccttag
     5761 cgggtgacgc cgtgctgccg ccaaaactgg cggcgcgcgt gctcgatgag tttgcccggc
     5821 tcgctcaaca agcggcacct gccgcgctac caattgtcac tcccgatctc accacgcgcg
     5881 aacgtgaagt gctcaatctg attgcgacgg gcgcaacgga caaggaaatt gctgcgcagt
     5941 tgtcgcttag cctccacacg gtgaagagcc atgtgcatag catcttgagt aagttgcatg
     6001 ccatcaatcg acggcacgcg gctgacctgg cgggtcgtca cattcacccc aagacgatca
     6061 agcgctgatt gttcaatttt caactaagaa ttagtcctga aggttgaggg cgatcaattt
     6121 gatcgccctt ttttcaatcc ccatccgggc gacgatggat agacacctga tcaagtttgc
     6181 cgtactctat caatatgaag acgcgcagac tcttcatcat cggtgattca cttttcgcgg
     6241 agacattaac tcaaatgtta tcgcgtgaag cgtccgtgcg catcatcggc tcggcgcctt
     6301 ctccgaatgc cgcgctgccg atgctatccg cctgtcagac cgatgccgtc gtcgtggccg
     6361 gcgaaaacga aatgtccacc gccgcctttg gtcaactcct cgccacctat cctgatttgc
     6421 ccgtcatctg cgccgatctc agccgcgaca gcgtgcaagt catcaccagt cattgcatcg
     6481 gcacgcgcgc ggccgatctt ttagctgcgg tcgccgagct gcccaaacgg aggtcatgaa
     6541 tgaactttgc ccctgctcaa ccgatcgagt ataccgagat cctttattcc aaagctgatt
     6601 ggatcgcgcg catcacgctc aatcgtccgc agaactacaa cgcgtactcg acgaacactt
     6661 tgatcgaatt agcggccgct tttcgtcaag cagcctttga cgatgaagtg gccgttatcg
     6721 tttataccgg cgcgggcgac cgcgcgttct gcaccggcgg cgatgtgaaa gagtatgaaa
     6781 gcacttatac gaaatcaccg cacgattatt ggaagtacat gggtttgttc agcgcgtaca
     6841 ttgaaagcat cttgaactct ggcaagcctg tcatcgcgcg gctgaacggc ctggccgtcg
     6901 gcggcggcaa tgaaagtcat ctggcttgcg atctatccgt catcgccgaa catgcatatg
     6961 tgggtcaagt gggcacgagc gtgggcagtg tggccgcggg cggcgcaaca caatggctgc
     7021 cgatcttcgt cggcgatcgg cgcgcgcgct ggatggtgat gctcaatcgt aaaatatctg
     7081 cgtatcaagc gctcgactgg ggcctggtga acgaagttgt tccgtcgatc aagaaagatg
     7141 gcgcattcat cgatcatgcc acgcctgaac aaattgccaa agcgagcaaa gggcaagacg
     7201 gttactcgat cgatcttgcg cggctcgacg agtctgtcaa tgccttgtgc gcggaactca
     7261 tcgataagtt tcctgagtgc atgcgctaca cgaaacagca gacaaacttc tggaaagatc
     7321 tggcctggca tcaaacggtc ggccatgcac gcgattggtt gtcgatccac tttgcgtcgt
     7381 gggaaccggt cgagggcatg ggcgcgttcg tcgagaagcg cgcgccgggc tatcgcatgc
     7441 tgcgcgaacg tgcggccaac ggtcaatcat ccgaatttcc gtggggaccg tatgcgaact
     7501 cgtgccccac ttgcggcagc aaatatctgc ccagcgattt tgaattttgc gggaagtgcg
     7561 gatcgaagtt gaacggtgcg tgatcacgtt atcactagcg gaggaactac atggccattc
     7621 gtgtcctcga tagcaaacct tacggaactt tacaagaagc gttgtattta tgccgcgact
     7681 tgctggaaga tacagatttt ccaacggtga agcgctggcg cgaagcgggc ggcaaagtgg
     7741 tgggacattt tcaagtttac ttcccggaag agatcgtcca cgccgccggt atgctgccgt
     7801 tcaaagtgcg cggtgcgccg atcgaagcgc ggcaggccga ggcgcgcttt ggatcatatt
     7861 tgtgctcgat tatcaaaacc tcgctcgaac tggtgctcag ccagcgcgta gaattggata
     7921 tgttcgtcac gcatcccatc tgcgatgccg cgcgcaactt gggcgcggtc tggggccgca
     7981 acttctctta tccgtgtcag attctgtatc tgccgcaaaa cgcgaactcg gctcacgccg
     8041 ccgaatatct gcgcggcgaa tacgatcgac tcaagcgcga catagaagcc atcgccggcc
     8101 attcgatcac tgacgacgcg ctgcgtgctt cgacgatttt gttcaacgaa aaccggcgtt
     8161 tgctgcgtca cctgtatgct atcaagcgcg aaacgccgtg gttgctctcg gccgctgaag
     8221 cctacatgct gaccgccgtc ggcggcttga tcccgcgcga agaacacaat gagttattga
     8281 aagctgtcat tccacagatc gagagtcgct ccgccaaacc gcaggatcgc atccgcgtcg
     8341 tgtttgaagg cggcttctgc gagcagcctc cgctcgatct gctgcacaac atctcgcagt
     8401 cgatctacgt ggtcgacgac gatctgctga tcgggct
//
LOCUS       DASRTJ010000087         6485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_266995,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000087 DASRTJ010000000
VERSION     DASRTJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266995"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFF70_01680"
     CDS             <1..1268
                     /locus_tag="VFF70_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43432.1"
                     /translation="PEFGSFKVIQVDMNGSGAREFAAELNTDHRLNADESILSPSDTL
                     LSVAELYARYGSTLEDRLGAALEASPDFVHLAAHWFPRALIADVNTGHLNLAEAILDM
                     NNGGPLPTAALLHDVGLPTEINLNLQTFSLNYALYHDERFDEVGPAGEVLWFLRRLEP
                     PSVLAIPPRLVYTAQLFDRTRIPGDLIKVEHSIDDELSDEAIPANDDDEITFTLTFPH
                     RVSGTLPLTPRIAKLFPTGRTHRIRFMFEDARTGSHWLGWVVREHTYAYGLEAWYIAN
                     DVPAGAYITLRRGTEPGTVIVDLQGQRQRREWVRVAAIKDSHLAFEMLRRPIPSNYDE
                     QMIIVAEDLIGADKLFTQIQERATPLEELIEKMLPNLAKLSPQGNVHAKTLYAAINVI
                     KRIPPGPLFAALANQSAFRSVGDGYWIAR"
     gene            1361..1798
                     /locus_tag="VFF70_01685"
     CDS             1361..1798
                     /locus_tag="VFF70_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43433.1"
                     /translation="MTTTPTKLNRFTKPTLDTRYHIDFDWWDKADRNLRVYLRSHLCE
                     EHRALYADSVETTEEIDWIDPQTAEVKRLDGLRLQMLTHCSKQPDFIAPHTSIIDAAF
                     RVFLTNNNESLSARELGEKINRDPELILRTLGGKQVYQGLRAI"
     gene            1871..2548
                     /locus_tag="VFF70_01690"
     CDS             1871..2548
                     /locus_tag="VFF70_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZY43434.1"
                     /translation="MKVAVKLHGILRDYRPNGVKIDLFELTLNDGAAVREAVEHFGIP
                     PKAVHAIFLNGELAEVDTLLKDGDQVRLFPPVVGGSEPRRRVFIAGIMQGSLRENSLL
                     EQNYRQTISSFLQQHVPNIEIVDPGTLHPNSVSYTAEDARRTLIELAQEAGRVDVLIA
                     FVPEASMGTALEMWEAYQHQRPIFTISPLIHNWVVKSLSTQVFADMDSFRAFVASGEF
                     ANTLRRI"
     gene            2574..2647
                     /locus_tag="VFF70_01695"
     tRNA            2574..2647
                     /locus_tag="VFF70_01695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2608..2610,aa:Arg,seq:tct)
     gene            2709..4460
                     /locus_tag="VFF70_01700"
     CDS             2709..4460
                     /locus_tag="VFF70_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY43435.1"
                     /translation="MLPKVSFRSILISIIILLIAAMPIGLAMARSARSEQRAVGELLT
                     NPGFEDPFIQAGTADLFVADGWAAWYITPDGVNFPSECPENAPATCVPYRVPVYHNTQ
                     PQDPHAPERARSGNSQQWGISYAVYIAGVYQHVANITPGTRLRFSAFMQGFNCSDNRG
                     CYGGIGQYAHSYEPGDMHMRVGIDPTGGQDPFGANVVWSGYQNPLDAFGLIQVEAVAQ
                     ANSVTVFLWSAPNYPELHTDIYVDDASLIAVSQGAVTPPAQPAGTAQPVPTVPSGPGT
                     YTIQPGDTLFAIAFANNLTLDQLLALNPGLTRDTVLQVGQVIKTGGATSASTPAPQST
                     SAPTIAPTSALAVASSTPGGPTTYTVKAGDTLSAIALQFNLTVDQLLSLNGITKDTPL
                     QVGQVLTVGVAAATPTPEATAAPTAAPTSASPLPTPTPAVSASGLCLLAFDDESEAGT
                     FTGSEALVTGVTFDVKGSDGKSLASYTSNGQQEPHCLSNLPDGRYTVDVTLPDGRQAT
                     TEIHWSLSLLSGTSVNVNIGTKPAPIDTATPAVTATPPTAAASQSGGGTFALIAGAAL
                     IVIAGIALFIGLRSRKK"
     gene            4595..>6485
                     /locus_tag="VFF70_01705"
     CDS             4595..>6485
                     /locus_tag="VFF70_01705"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HZY43436.1"
                     /translation="MRNNALRNFLRTPHFTLGMAALIALSIAYQFRAPITLDMASNDE
                     EIYLTRGFYPPEETFGVTYRWTSGEARIVLPGVGSGTSLHLHVNLQELRPSSLAPQPV
                     TISLNDRSVITFTPTTQLNGYDFDLPASDLRGDVILNFQSDTFIPKNSIANNTDDRSL
                     GLFIDQLQITDGSGLVVPPLIVWLLLAGAVLGAYAFGQAVNWGTPINVGIGIVGLLLE
                     IIGIVAARTWTAHNAIWWTGSIVAAWLIAQRLKRPKTEERRQQLANDQQQLNHSQGST
                     AVSSQRSVVKIPYLNILIVFLAWRLALIAIPIVGSDVIGTRECCPEVLPKPLTSWSQA
                     AFGDWYRWDAIWYGSIAQNGYQYFGTREASNVAFFPGFPLLNGIISHTIGLPVEVSGP
                     VVSTLLALIACYFLYRLTLRETDDADTAYRSIVYFLAFPAAYYLAIGYSEALYVVCVL
                     AAFLFAREGKWGWCAVAAFFAGLARLHGALLIVPLAYEYWRHASRRSIARKNISSVFA
                     APIGVLAFMGYLGAQFGKPLAYFEIQTLFFKGIRADAFPTFPGTTFANYLNGLLTGTP
                     STESVAVMGAMILLIVLTIEVWARLPKIYGVYMLTVTLFSLASGDLISMPRFVVPMFP
                     GFITLA"
BASE COUNT         1379 a         1894 c         1730 g         1482 t
ORIGIN      
        1 atccagaatt tggatcgttc aaagtcattc aagtcgacat gaacggcagc ggcgcgcgtg
       61 aatttgcggc cgaactcaac accgatcacc gcctcaacgc cgacgaatca atcctgtcgc
      121 cgagcgacac gttgctgtcc gtcgctgagc tttatgcacg ctatggctca acgcttgaag
      181 atcgtttagg tgcggcgttg gaagccagcc ccgattttgt ccacctggcg gcgcactggt
      241 tcccgcgcgc gttgatcgcc gatgtgaata ccggccacct caatctggct gaagcgatcc
      301 tcgatatgaa taatggtggg ccgctgccca ctgcggcctt gctgcatgac gtggggctgc
      361 ccaccgaaat caatcttaac ctgcaaacct tctcgttgaa ctacgcgctg tatcacgatg
      421 agcgcttcga tgaagtcggg ccggccggtg aagtcttgtg gttcttgcgt cgattggaac
      481 cgccttcggt tttagcgata cccccccgat tggtctacac ggcccaactg tttgatcgaa
      541 ctcgcattcc cggcgatctg atcaaggttg aacattcgat cgatgatgag ctatcagatg
      601 aggccatacc cgcgaacgat gacgacgaga tcactttcac attgacgttt ccacatcgcg
      661 tcagcggcac actgccgtta acgccgcgca ttgccaaatt atttcccacc ggccgcactc
      721 atcgcattcg cttcatgttc gaagatgcgc gcacgggctc acactggttg ggctgggtcg
      781 tgcgcgaaca cacatacgcc tacggcttgg aagcgtggta catcgccaac gatgtgcccg
      841 ccggcgctta tattacattg cggcgcggta ccgagcccgg caccgtcatc gtcgatctgc
      901 agggccagcg ccagcgccgc gaatgggtgc gtgtggccgc aatcaaagac agccatctgg
      961 cctttgaaat gctgcgccgc cctatcccct cgaactacga cgaacaaatg atcatcgtcg
     1021 ccgaagattt aatcggcgca gacaagctgt tcactcaaat tcaggaacgc gctacaccgc
     1081 ttgaagagct gatcgaaaag atgctgccca atctggcgaa gctcagtccg cagggcaatg
     1141 tccacgccaa aacgctgtac gccgcgatca atgtgatcaa gcgcataccg cccggcccgt
     1201 tgtttgccgc gctcgccaat caatctgcct tccgatcggt tggcgacggt tattggatcg
     1261 cccggtagag acggcttgaa cgaatcctct ccgcacaatg aacgatggta taatcatcga
     1321 cggctaatac agccgtcgaa tttattttaa gataacgatc ttgacgacga caccgactaa
     1381 gctcaatcgt ttcaccaaac cgacgctcga tacccgctac cacattgact ttgactggtg
     1441 ggacaaagcg gatcggaatc tgcgcgtgta tctgcgcagc cacttgtgcg aagagcatcg
     1501 cgccttatat gccgactcgg tcgagacgac tgaagagatc gactggatcg atccgcagac
     1561 ggcggaagta aagcgcttgg acggcctgcg cttgcagatg ttgacgcatt gctccaagca
     1621 gccggatttt atcgcgccgc acaccagcat catcgatgcg gcctttcgag ttttcttaac
     1681 gaacaacaat gaatcgttga gcgcgcgcga attaggcgag aagatcaatc gggatccgga
     1741 attgatcctt aggacgctgg gcggcaaaca agtctatcaa ggcctgagag caatttagaa
     1801 acctggaggt tttgcccact ccgcttcgct acggggtgct tcgcaaaaac ctcgcaggtt
     1861 tggggcgaat atgaaagtcg cagtcaagct gcacggcatc ttgcgcgatt atcgaccgaa
     1921 cggcgtcaag atcgatctgt ttgagctaac gctaaatgac ggcgcagcgg tgcgcgaggc
     1981 ggtggagcat ttcgggattc caccgaaagc cgtccatgcg attttcttaa acggtgaact
     2041 ggctgaagtg gatactcttt tgaaagacgg cgatcaggtg cgcttgtttc caccggtcgt
     2101 gggcggcagt gaaccgcgcc ggcgcgtttt cattgcaggc atcatgcaag gttcgctccg
     2161 cgagaacagc ctgctcgagc agaattatcg gcagacgatc agctcgtttt tgcagcagca
     2221 tgtgcccaac atcgagatcg tcgatccggg cacgctgcac ccgaacagtg tcagctacac
     2281 tgctgaagac gcgcggcgaa ctttgatcga gcttgcccaa gaagcgggcc gcgttgatgt
     2341 attgattgca tttgtgccgg aagccagcat gggcaccgcg ctggagatgt gggaagcgta
     2401 tcaacatcaa cgaccgatct tcaccatctc gcccttgatt cacaactggg tggtgaagag
     2461 tttatcgact caagtgtttg ccgatatgga ttcattccgc gcgttcgtcg ccagcggaga
     2521 atttgccaac accctgcgac gcatttgaga ggcgcgttgc gccttaacct cctgccctcg
     2581 tagctcaatt ggatagagcg acggacttct aatccgaagg ttgtgggttc aaatcccgcc
     2641 gggggcacct gatcaaattt attccacgca ccgggccgca cccggcctca aaagttggag
     2701 ggccaacaat gttacccaaa gtctcatttc gttccattct tatttcgatc atcatacttc
     2761 tgatcgcggc catgccgatc ggtttagcga tggcccgatc ggcgcgaagc gaacaacgag
     2821 ccgttggtga gctgctgacc aatcccggct tcgaagatcc tttcattcaa gcgggtactg
     2881 ctgatctctt cgtcgccgat ggctgggcgg cgtggtacat cactcccgac ggtgtgaact
     2941 ttcccagcga gtgtcccgag aatgcaccgg ccacctgtgt accttatcgc gttccggtct
     3001 atcacaatac gcagccgcaa gatccacacg cgccggagcg cgctcgatcc ggcaactccc
     3061 agcaatgggg cattagctac gcggtgtaca tcgccggagt ttatcagcat gttgccaaca
     3121 tcacgcccgg cactcgactg cgcttcagtg cgtttatgca aggcttcaat tgcagtgaca
     3181 atcgcggctg ctacggtggc atcggacaat atgctcattc ttatgaaccg ggcgatatgc
     3241 acatgcgcgt cggtatcgat ccaacaggcg gccaggatcc atttggcgcg aatgtggtat
     3301 ggtccggcta ccaaaatccg ctcgatgcct ttgggttgat tcaagttgaa gcagtcgctc
     3361 aagccaactc tgtgactgtc ttcctctggt cagcaccgaa ttatccggag ctgcacaccg
     3421 atatctatgt cgacgatgcg agtttgatag ccgtgagtca aggtgcggtc acgccgccag
     3481 cgcagccagc gggtaccgct cagcccgtgc cgactgttcc ctcaggcccc ggcacttaca
     3541 ccattcaacc tggcgacaca ttgtttgcca tcgccttcgc caataatctc acactcgatc
     3601 aattgctcgc gctgaatccc ggcctgacgc gcgatactgt tttgcaagtc ggccaagtga
     3661 tcaagacagg tggcgccacc tccgcttcaa cgcctgcgcc gcagtccact tcggctccaa
     3721 ctattgcgcc tacttctgca ctagcagtgg catcatccac acccggtgga ccgacgacgt
     3781 acacagtcaa ggcgggcgat acattatctg cgattgcgct gcagttcaat ctgactgtcg
     3841 atcagctgct gagcctcaat ggcatcacca aagatacgcc gctgcaagtt ggacaagtat
     3901 tgacggttgg cgtagcagcc gccacgccaa cgcctgaagc aacggccgcg cctactgctg
     3961 cgcctacctc tgcttcacct ctaccgacgc ccacaccggc ggtatctgca tcgggcttgt
     4021 gcttattggc ttttgatgac gagagtgaag ccggcacttt caccggcagc gaagcattgg
     4081 tcacgggtgt gacattcgac gtcaagggtt ccgatggtaa atcacttgct tcctatacaa
     4141 gcaatggcca gcaagaacca cactgtctta gcaatttgcc cgacggccgt tacaccgtcg
     4201 atgtaactct gcccgacggt cgacaagcta cgacagagat ccattggtcg ttgtcattat
     4261 tatccggcac atcggtcaac gtgaatatcg gcaccaagcc agcgccgatc gatacggcca
     4321 cgcccgcagt gacagccaca ccgccaacag ccgcagcgag tcaaagtggc ggcggcacct
     4381 ttgcattgat cgccggagcc gcgctcattg tgatcgccgg catcgctttg ttcatcggct
     4441 tgcgttcacg caagaagtaa gcgcaaaacc tggcgcactc attggcaatt gcgcggattg
     4501 attgcactcc gcgcatcttc tgctcactca gtcgttgcac gacgcgatca aaagccgatc
     4561 ctctgcggtt atcaaccctt gagatcaaac tgtcatgcgt aacaacgcgc tgcgcaattt
     4621 tcttcgcact ccacatttca cgcttggcat ggcggccttg atcgcgttgt cgattgctta
     4681 tcaatttcgc gcgccgatca cgctcgacat ggcctcgaac gacgaagaga tatatttgac
     4741 acgcggcttc tatccgccag aagaaacatt tggcgtgacg tatcgctgga ccagcggaga
     4801 ggcgcgaata gttttgcccg gcgtaggcag tggaacaagc ttgcatctgc atgtgaactt
     4861 gcaggaactc cgcccttcgt cgctcgcgcc acagcccgta acgatttcac tcaacgatcg
     4921 atccgtcatt actttcacac caacgaccca actcaacggt tatgacttcg acctgcccgc
     4981 gagcgatctg cgcggcgatg tcattctcaa ctttcagtct gatactttca ttccaaaaaa
     5041 ttcgatcgcg aacaataccg acgatcgatc gttgggcttg ttcatcgatc agcttcagat
     5101 cacagatggc tcgggcttgg tcgtgccgcc gctgatcgtc tggttattat tagctggcgc
     5161 ggtgttgggg gcatatgctt tcgggcaggc cgtaaactgg ggaacgccga tcaacgtcgg
     5221 gatcggaatc gtcgggctgc ttcttgagat catcggtatc gttgcggcac gcacctggac
     5281 tgcacacaac gcaatttggt ggaccggatc gatcgtggcg gcttggttga ttgcgcagag
     5341 actaaaacga ccgaagacag aggagcgccg acagcagtta gcgaatgatc agcaacaact
     5401 taaccacagc caaggatcaa cagcggtcag cagtcagcgg tctgtggtca agattccata
     5461 tctcaacatc ctcatcgttt ttctcgcgtg gcgactcgct ctgatcgcaa ttccgatcgt
     5521 cggctcggac gtgatcggca cacgcgaatg ttgtcccgaa gtattaccaa aaccgttgac
     5581 ctcttggtcg caagccgcat tcggcgattg gtatcgctgg gatgcaatct ggtatggcag
     5641 catcgcacag aacggctatc aatacttcgg cacacgtgaa gcctcgaacg tcgcgttctt
     5701 ccccggcttc ccgctgctca acgggatcat cagccacacg atcgggttgc ccgtcgaggt
     5761 cagcgggccg gtcgtgtcaa ccctgctggc gttaatcgcg tgctacttct tatatcgact
     5821 cactttgcgc gagaccgatg atgcagacac tgcatatcga tcgatcgttt atttcctggc
     5881 atttccggcg gcatattact tggcgatcgg ttacagcgag gcgttgtacg tggtgtgtgt
     5941 cttggccgcg tttcttttcg cgcgtgaagg caagtggggt tggtgcgcgg tcgccgcctt
     6001 cttcgccggc ttggcgcgct tgcatggtgc cttgctgatc gtgccgctgg cttatgaata
     6061 ttggcggcac gcttcgcgtc gatcgatcgc gcgcaaaaac atttccagtg tattcgccgc
     6121 cccgatcggt gtgctggcct tcatgggtta tctcggcgcg cagttcggta aaccgctggc
     6181 ttacttcgaa attcaaacgc tgttcttcaa aggcattcgc gccgacgcgt tcccgacttt
     6241 ccccggcacg accttcgcca attacttgaa tggcttgttg accggcacac cttcgaccga
     6301 aagtgtcgcc gtcatgggcg cgatgatctt attgatcgtg ctgacgatcg aagtgtgggc
     6361 gcgcctgccg aaaatttacg gcgtgtacat gttgaccgtc acactcttct ctctcgctag
     6421 cggcgatttg atctcgatgc cgcgcttcgt tgtgcccatg ttccccggct tcatcacctt
     6481 agcac
//
LOCUS       DASRTJ010000088         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_231849,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000088 DASRTJ010000000
VERSION     DASRTJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231849"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFF70_01710"
     CDS             <1..601
                     /locus_tag="VFF70_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43437.1"
                     /translation="LTEVYTGTFNPFGAPSSSGYDAVNDTYNLTFTNGSAGDSDLVRL
                     GACADRSSLRLGLPSPAFYWTLTGTRTLPDPLSAGLNLNWVDHTHVQIDLFNEQAITL
                     TLWSLNVLDPDQKLALDDLSDDIAALLPMASEVVTDPITLLPSANPSFNTAVLLGNHP
                     YVIEAVLSAEDDPGNLIHVLAQITSPAFNLYLPLVLKNF"
     gene            complement(722..1369)
                     /locus_tag="VFF70_01715"
     CDS             complement(722..1369)
                     /locus_tag="VFF70_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HZY43438.1"
                     /translation="MVFLSILQKNNNREWFEQNRSKYETAKLQFEMLVDELILEYNDV
                     EDMRGVRAKDCVMRIFRDVRFSKDKSPYRTSLGASIGPGGKRSTRMAYYLHLEPHDKS
                     MIAGGLYLPESAHLTAFRTSIDRDASKFKKIINDTAFKKYFGSIDANEKLKTAPQGYA
                     KDHPEIDLLRLKQVTVARLFTDEEVLSKLFTTQAIKTFKVMKPFLDYFNGSLSWQ"
BASE COUNT          349 a          457 c          396 g          411 t
ORIGIN      
        1 gctcacggaa gtgtataccg gcacgttcaa tcctttcggc gccccatcat ctagtggcta
       61 cgatgccgtt aacgacactt acaatctcac tttcacgaat ggctccgcgg gcgatagcga
      121 cctggtgcgc ctgggtgcct gcgccgatcg atcgagcctg cgcttgggcc taccctcacc
      181 tgctttctat tggacgctga ccggcactcg cacactccct gatccgttat ctgccggttt
      241 gaatttgaat tgggttgatc acacgcatgt gcagatcgat cttttcaacg agcaggcgat
      301 cactttaacg ctgtggtcgt tgaatgtgct cgatcccgat caaaaactgg cgcttgacga
      361 tttgagtgac gacatcgccg cgctgctgcc gatggcgagc gaagttgtca ccgatccgat
      421 cacgctgctg ccaagcgcga atccatcgtt taataccgca gtgctgttgg gaaatcaccc
      481 gtacgtaatc gaagcggtgt tatccgccga agacgatcca ggcaacctga ttcacgttct
      541 cgcgcagata acatcaccgg cctttaacct gtatttgccg ctggtgttga aaaatttcta
      601 aagctctgcg ctgtgtcaat tcgccagtat aaaaatcgag actgctcact gagcagtctc
      661 gatacattta tgactaaaac gccttcgagg cacttcacca aacttcagaa gccgtatcga
      721 ttcactgcca gctcaagctg ccattgaagt aatcgaggaa tggcttcatc accttgaaag
      781 ttttgatcgc ctgcgtcgtg aataacttcg acaggacctc ttcatcggtg aacaggcgcg
      841 ccacggtcac ctgcttgagc cgtagcagat cgatctccgg gtgatccttc gcataaccct
      901 gcggcgctgt ctttagtttc tcgttcgcgt cgatcgagcc gaagtatttc ttgaacgctg
      961 tgtcgttgat aatctttttg aatttactcg cgtcgcgatc gatcgatgtg cgaaaagcag
     1021 tcaagtgggc cgactccggc agatacaggc cgcccgcaat catcgatttg tcatgcggct
     1081 ccagatgcag atagtacgcc attcgtgtcg atcgtttgcc gcccggcccg atcgatgcgc
     1141 ccaggctcgt acgatagggc gatttatcct tcgagaatct cacatcacga aaaattctca
     1201 tcacgcaatc cttcgcccga acaccgcgca tgtcttcgac gtcgttgtac tctagaatca
     1261 gttcgtcaac cagcatttca aactgcaact tggccgtttc atatttggat cgattctgct
     1321 caaaccactc gcgattgtta ttcttctgca aaatgctgag aaaaaccaaa acgggcttaa
     1381 gatcgatcgg atcgcttatc gggatttcgc tccaagtgtc tcggaaataa agcgaggcga
     1441 ctcagtgagt cgcctcgaac cgcaatcaat tcaactgttg ctcaggccgg aaccggtgtg
     1501 ggctgcttgc caccgccaga tggtgccggt gattccggcg ccggcttgtt gggcttcggc
     1561 gcgggcgtac tgtcgtattt ttccgggcct ttcggctgct caccattcca cac
//
LOCUS       DASRTJ010000089         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307715,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000089 DASRTJ010000000
VERSION     DASRTJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307715"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..2908)
                     /locus_tag="VFF70_01720"
     CDS             complement(122..2908)
                     /locus_tag="VFF70_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43439.1"
                     /translation="MSTSISNLAKDNTPKVAPSSRLPRRRWISVRLRFLILVVGSGLI
                     ILGLGSFVIFNIAQNVALAEVRLRTNEIVNNTALQLNDRLVRLSESAHGAAAAAYAEA
                     GDLTKLRTTLSQIGQRELDFLSIYVFFDRQAIAGHDYAAVWETRDTMNANRTTKPYYP
                     NLIGAAQYEPNLPVYDYFQNNPRFDVGKNADKGRVIWTDNFPDKGGPGNTNVVAGVTP
                     IYDASDHFTGIAGIDLDFSQIRTYVAAIKPTANSYAMLLDRNGLLIAHAKDTQLNALG
                     QAVSQIAQDNNSTDLKQLGDQMTSGQSGVTELTDPLTGIDVFAAYHPIDTTGWSIAVL
                     IPIADVTGNVNQMGGSILALSLTALGAFGLIGLLLSETVVRPLIRLTKTATLMSGGDL
                     KQIAVIERNDEIGTMASTLNTMTSRLSDLIDSLENRVELRTAQLQASADVGQAATSVL
                     DADQLLKTVVNLITDRFGFYYAAAFIVDDSRHWAVLAEATGEGGRKLKERGHKLEVGG
                     QSMVGSSIAERKPKIALDVGAVAVRFANPLLPDTRSEIALPLILGDHVLGALDVQSTL
                     AAAFDETSTAVLQSMADQVAVALNNADQYQREQARSHQTAALLTATLELTGEVDRSSL
                     ADRIVQLSTRILKADGAGLWLINNNTLGLTAALSASGPAATAKLTPHPGDQLAQRVYT
                     SGQIIRVDDYAGWLSLNDSSLTPDLMHAAIGVPLFWRNQINGVLVISQSEPDLRFTDN
                     DESAAQLLASQAAAAIANVMLLQQQEQTLKELNIVNRRLTGEAWQSHLQGHAIVYEAK
                     RSAAQLIEPALSLQIPVALRGQAIGAITIEDDDSQRTLTDDERGLIHGIIQQMTLALE
                     NQRLTEVTQLAAQRDRAIAEAADKIHRPTDLDAVLQVAVEEISRIVGTRDVRIRLGVD
                     TSPGDGNGRQNVHA"
     gene            complement(2972..>3576)
                     /locus_tag="VFF70_01725"
     CDS             complement(2972..>3576)
                     /locus_tag="VFF70_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43440.1"
                     /translation="VVVQSQQLLAETQTTLKQLDAINRRLTGQAWRDYTMTNGSLRIV
                     DAGLGVPTTANNLSESVVNADRVQVVGPIGDTRSGLIAPIALRGEVIGALSLQEIDKD
                     REWTENEITLLQTVANDVAVAIENARLIEQTERRAERELVISDVSTRMFAANDLNNIV
                     KLAGDELARVLHAARTEVQISGEYLSLIADEASIDDHHGQ"
BASE COUNT          781 a          934 c          982 g          879 t
ORIGIN      
        1 gatcggcagt tggcggagaa tcaaggtgac cagtgtagcg attagcggaa acactaaacc
       61 aaaagctgcg ccgagggcaa cataacggcg cgtctcggtt gttagccagc gagtgagcag
      121 gttacgcatg aacgttctgc cttccgttgc catcacctgg cgatgtgtca acacccaatc
      181 gaatgcgcac atcacgcgtg ccaacgatgc ggctgatttc ttcgacggcc acttgcagta
      241 ccgcgtccag atcggtaggc cggtggatct tatcggcggc ctcggcaatc gctcgatcac
      301 gctgggcggc tagttgggtc acttcggtta agcgttgatt ttccagagcc agcgtcatct
      361 gttgaatgat gccgtgaatc aggccgcgct catcgtcggt cagagtccgc tggctgtcat
      421 catcctcaat cgtgatggcg ccgatcgctt gaccgcgcaa ggcaacgggg atttgcaggg
      481 acagggctgg ttcaatcaac tgtgccgcgc tgcgcttcgc ttcgtagacg atcgcatgtc
      541 cttgcaaatg agattgccaa gcctcaccgg ttaagcggcg attaacgatg tttaattcct
      601 tcagagtttg ctcttgttgt tgaagcagca ttacgttggc gattgctgcc gcagcttggg
      661 aggccaataa ctgagcggca ctttcgtcgt tgtcagtaaa ccgtagatcg ggctcagatt
      721 ggctgatgac caacacgcca ttgatttgat ttcgccagaa taatggcaca ccgatggccg
      781 catgcattag atcgggagtc agactactgt cgttcaggct aagccagccg gcataatcgt
      841 caactcggat aatttgtccg ctggtatata cacgctgcgc taattgatcg ccggggtgtg
      901 gcgtaagctt agcagtcgct gccggtccgc ttgcgctaag cgctgcggtc aatccaagtg
      961 tattgttgtt gatcagccac aagcccgctc cgtcagcctt gagaatgcgg gtggatagct
     1021 gcacgatacg atcagccaat gaagatcgat caacctcgcc ggttaattcc aaagtggctg
     1081 ttaacaaagc ggccgtttga tgagatcggg cttgttcgcg ctgatactgg tctgcgttat
     1141 ttagcgcaac ggcaacttga tcggccatgg attgcagcac tgcggtgctt gtttcatcga
     1201 aggccgctgc cagagtcgat tggacatcta gcgcgcctaa cacgtggtcg cccaagatta
     1261 agggcagggc aatctccgat cgggtatctg gcaataacgg attggcaaag cgcaccgcca
     1321 cggcgccgac atccagcgca atcttgggtt tacgctcggc gatcgaactg ccgaccattg
     1381 attgtccgcc gacctctaat ttgtgtccgc gctctttgag cttgcgcccg ccctcgccgg
     1441 tggcttcagc cagaaccgcc caatggcgag agtcatcgac gataaatgcg gccgcgtagt
     1501 agaaaccaaa ccgatcggtt atgagattaa cgaccgtctt taacaattga tcggcatcta
     1561 gcactgaagt cgctgcctgg cctacatcgg cactggcttg tagttgcgcg gtgcgcagct
     1621 cgacgcggtt ttccagcgag tcgatcaaat cgcttaagcg gctggtcatc gtattcaggg
     1681 ttgaggccat cgttccaatt tcatcattgc gctcaatgac cgcgatttgt ttcaagtcgc
     1741 caccactcat gagtgttgct gtcttggtta agcggataag tgggcgcacc acggtctcgc
     1801 ttaataacaa accgatcaag ccgaaggcgc ccagcgcggt gagtgagagc gctaaaatgc
     1861 tgccgcccat ctgattgaca ttgccggtca catctgcgat cgggataagt actgcgatcg
     1921 accagcctgt cgtgtcgatg ggatgataag cggcgaagac gtcaatgccg gtcagcggat
     1981 cggtcaattc ggttacgcct gattgtccac tggtcatctg atcgccaagc tgcttcagat
     2041 cggtactgtt attgtcttgg gcaatttgac tgactgcttg gccgagggcg ttgagctgcg
     2101 tgtctttggc gtgagcaata agtaacccat ttcgatcgag cagcatagcg tagctattag
     2161 ccgtgggttt tatcgccgca acataagtgc gaatttgact aaaatctaaa tcgatgcccg
     2221 cgataccggt gaagtgatcg ctcgcatcat aaataggcgt gacaccggca acgacgtttg
     2281 tattgccggg tcctccttta tctggaaaat tatctgtcca gattacgcgg cctttatccg
     2341 catttttacc gacatcaaag cgtggattat tctggaagta gtcatacacc ggcaaattgg
     2401 gctcgtactg cgcagcgcca atcagattag gataatatgg tttcgtcgtg cgattggcgt
     2461 tcatcgtatc acgggtctcc cagactgcgg cataatcgtg acccgcgatc gcctgtcgat
     2521 caaagaacac gtaaatgctg agaaaatcca attcacgctg cccaatttgt gagagggtcg
     2581 ttcgtagctt tgtgaggtcg ccagcttcag cataagccgc tgcggctgct ccgtgagcgc
     2641 tttcgcttaa gcgcaccaat cgatcgttaa gctgcagcgc tgtattgttg acgatctcat
     2701 ttgttcgtag cctgacttca gctaatgcta cgttttgagc aatgttaaag atgacgaagc
     2761 tgccaagacc caaaataatc aggccagagc caacgactaa aatgaggaaa cgcaagcgca
     2821 cactgatcca gcggcggcgc ggcagacggc tagacggcgc aactttgggg gtgttgtctt
     2881 tggccaagtt ggagatggat gtgctcatgc actcgcctcc ataaccagcc actcgggccg
     2941 gatcgattgt ttcgttgaat cgctgttttg attattgtcc atgatgatca tcgattgaag
     3001 cttcgtcggc aattaagctg agatattcac cgctaatctg tacttcggtg cgcgcggcat
     3061 gcaggacacg cgctaattca tccccggcca atttgacgat gttgttaaga tcattcgcag
     3121 caaacatgcg ggtgctgaca tcgctgatga caagttcgcg ttcggcgcgt cgttcagtct
     3181 gctcaatcaa gcgggcattc tcgatggcga cagctacgtc attggcgacg gtttgcagca
     3241 atgtaatctc gttctcagtc cactctcgat ccttatcgat ctcttgcaga ctgagcgcgc
     3301 cgatgacttc gccgcgtaat gcaatcggcg caattaaccc ggagcgggta tcaccgatcg
     3361 ggccgaccac ttgcacacga tccgcattga caaccgattc gcttaagttg ttggcggttg
     3421 tggggacgcc cagaccggca tcaactatcc gcaggctgcc gttggtcatt gtgtaatcgc
     3481 gccaggcttg accggtcaag cggcgattga ttgcgtctag ctgtttcaaa gttgtttggg
     3541 tctcggctaa aagttgttgg ctttgcacca caaccg
//
LOCUS       DASRTJ010000090         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307733,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000090 DASRTJ010000000
VERSION     DASRTJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307733"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFF70_01730"
     CDS             <1..511
                     /locus_tag="VFF70_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HZY43441.1"
                     /translation="KNLVNSYPPNNFDRQFSFEEVGRLQQSLEAMYGPRGARGLALRA
                     GRACFKYGIREFGPVLGIADLAFRLLPLGMKLKVGFEVFAETFNKFSDQVVRLGEERD
                     RFRWIIDRCPVCWGRQTEEPCCHLAVGILQEGLYWVSSGKNFLVEEITCIAKGDKECV
                     IVIEKRPLD"
     gene            532..1821
                     /locus_tag="VFF70_01735"
     CDS             532..1821
                     /locus_tag="VFF70_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HZY43442.1"
                     /translation="MSAKIRNLRRIVIRDTRPLPPFNEPARDLRVLNKPLWLMQRDVL
                     APYTREEREISSIEAMSADRVESIVYRDNLFFDQAFIDYFLNRARELGKACRVAFSPN
                     DPAIKIHALPLQSGIRLEKGFAEGELLVADMWYFPYGRESAPRPLVIDTQAREIGYYN
                     VPKYMAPNQGDLTYQVPQRAFLSIEHWVHLFLANSTFGVFADGLRAEKRIEQTGFKLH
                     VLLSAMAQRKRVLSSPALVKVGKNVHIDPTAIIHGPTTIGNNVTVGPGAVIDNCIIGN
                     NVNVAQGCELMLSVVGDGCFLPFRAALFMTTLMENSMVAQNTCLQMCIIGRDTFIGAG
                     STFTDFNMLPKAIRTLYRNELVQVGLPVIGGCVGHNCRLGSGLIIFPARMIESDVLLL
                     GSRAERVITHNVAYQDSDHLKLPNGERLHPAQYRLRV"
     gene            1856..2968
                     /locus_tag="VFF70_01740"
     CDS             1856..2968
                     /locus_tag="VFF70_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZY43443.1"
                     /translation="MDNFAFIIHPIDPKRDVQRKFPVLGKVLPVRMINFLSRFFPPLY
                     ISHITGIRSSATGKEIEGWFVACPFTPQRMMTLPARIVYRKIIATAKKAQSLGAQLVG
                     LGAYTSVVGDGGVTIGHMVEIPVTTGDSYTVAIAIEALKKAAKQMEIDLSSATAAVVG
                     ATGTIGAVSAELLAQAVPQIILIGRNSDRLGLVKARCEAAGAEVQVATDLNALRQADL
                     VLTVTSAMDSIIEPEHIKSGAVVCDVARPRDVSRQVIEQRDDVLVIEGGMVEVPGEVD
                     FHFNFGFPPRMAYACMAETMVLALDGRYESYSLGKDLTIEQVQEIDRIARYHGFTLGG
                     FRSFENAVSAEQIEQIKLKARSKRLGLKTVAAISAA"
     gene            3020..3910
                     /locus_tag="VFF70_01745"
     CDS             3020..3910
                     /locus_tag="VFF70_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43444.1"
                     /translation="MSKGRILVVEDDLDISNMLRIYFTSQGYEVTVAGRGLEALEQTR
                     KQLPSVIVLDIMLPDIDGYEVCAKLRSALRTSHVPIIFLTQKDERSDRIAGLELGADD
                     YITKPFDIEELRLRVQNAMRRAERESLTNPTTGLPSGKLIEEQLRGLMRRKGWGLLYI
                     GLTDLDAFNDVYGFVAGDEVLRFTALLLGETDDKVGGPDDFIGHVGGDDFIVITTDEK
                     AQALADAAHKRFSQDIGSHYSFKDREHGYIVVKDTDDKERQASLIKMCIGVIFANTQE
                     FTDIREITEVSAEARRQMCS"
     gene            4105..>5287
                     /locus_tag="VFF70_01750"
     CDS             4105..>5287
                     /locus_tag="VFF70_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZY43445.1"
                     /translation="MQISFGRLGVRLWVPFVIAAIVSVAIVALLVVVSIDNQSAQISS
                     TQRSVAQSLVTQVSGFVERVRDDLKRLVNPATLPSLSRLVLDQVLSNAIRDQAAFSDL
                     SLVGLNGFEIARVARNDNTIIILTDRSGSAEFQTAAAGREFIGPVRTIEGVPTVRIGV
                     PIFNDRQDVIDVVVGEVDLQSLCDVVCTVSFGLQGYAYLIDDHGNLIAYRDFKSVVSP
                     VTYMPAANDAIINHGQLTGPQLFDTGLASHGESTLAYYAPVLVTDSSRWYIVLEQPTV
                     DAFAQLNELILIGIGLVLISLGSIVVMGILISRRVMRPLAMLRRGAAQLARGDLHHRI
                     TGVQTGDELQFLAAEFNEMATKLQEAQSNSASVALERQQQFEVVQRRVKEMSTLLQTG
                     RA"
BASE COUNT         1176 a         1242 c         1552 g         1317 t
ORIGIN      
        1 gaagaatctg gtcaatagct atccgcccaa taacttcgat cgccagttta gctttgaaga
       61 agttggtaga ttgcaacaat ctctggaagc gatgtacggt ccgcgcggcg cgcgcggttt
      121 ggcgttgcga gctggtcggg cgtgtttcaa atatggcatt cgcgagttcg gtccagtctt
      181 gggtattgcg gatctggctt ttcgattgct gccgctgggt atgaagttaa aagttggctt
      241 tgaagttttt gcagagacct ttaacaagtt cagcgatcaa gtggtgcggc tgggtgaaga
      301 acgcgatcga ttccgctgga ttatcgatcg ttgcccggta tgctggggtc gccagacaga
      361 ggaaccgtgt tgccacttag cggtcggcat tttacaggaa ggcttgtact gggttagcag
      421 cggcaagaac tttctggttg aagaaataac ctgtatcgcc aagggcgata aagaatgcgt
      481 gatcgttatt gaaaagcgtc cgctagatta aggtgaggtt ggaattccgg aatgtcggcc
      541 aaaattcgca acctccgtcg aattgttatc cgcgatacgc ggccactgcc accgtttaat
      601 gagcccgcgc gcgatctgcg cgtgctgaat aagccgctgt ggttgatgca gcgcgatgta
      661 ctcgcaccat acacccgcga agaacgcgag atctcctcga tcgaggccat gtcggccgat
      721 cgagttgagt ccattgtgta tcgcgacaat ctcttcttcg atcaagcatt cattgattat
      781 tttctcaata gagctcgcga gctgggtaaa gcctgccgcg tcgcgttctc gccaaacgat
      841 ccggcaataa aaattcatgc gctgcccttg cagagcggca ttcggctgga gaagggtttc
      901 gccgagggcg agctgctggt ggccgatatg tggtactttc catacggtcg cgagtcagct
      961 ccgcggccgt tagtgatcga tacgcaagca cgcgagatcg gatattacaa tgtgcccaag
     1021 tatatggcgc ccaatcaagg tgatttgact taccaagtgc cgcagcgggc ctttctgtcg
     1081 atcgagcatt gggtgcactt gttcttagct aattcaactt ttggtgtatt tgccgacggg
     1141 ctgcgcgcgg aaaaaaggat tgaacaaact ggcttcaagc tgcatgtctt gctgtcagcc
     1201 atggctcagc gtaagcgtgt attgtccagc cccgcgctgg ttaaagtcgg aaagaatgtt
     1261 cacattgatc cgacggcgat catccacggg ccgacgacga tcggcaataa tgtcaccgtc
     1321 ggaccgggtg cagttattga taattgtatc atcggcaaca acgtcaatgt tgcgcagggc
     1381 tgcgagttga tgttgagcgt cgtgggtgat ggctgcttcc tgccctttcg cgcggcgttg
     1441 ttcatgacca cgttgatgga aaattcgatg gttgcgcaga atacctgttt acaaatgtgc
     1501 atcatcgggc gcgatacatt tattggcgcg ggctcaacgt ttaccgattt caacatgctg
     1561 cctaaagcaa ttcgcaccct gtatcgcaat gaactggttc aggtgggact gcccgttatc
     1621 ggaggctgtg ttggccataa ctgccgactt ggctccggcc tgattatttt tccggcacgg
     1681 atgatcgagt ctgacgtgct gttgttgggc tcgcgtgccg agcgggtgat tacacacaat
     1741 gtggcctatc aagacagcga tcatttgaag ctgccgaatg gcgaaagact gcatccggca
     1801 caatatcgat tacgtgtgta gagccgacag gtcgggtcgg ctgcggggga tttagttgga
     1861 caacttcgca tttatcattc atccgatcga tcccaagcgg gatgtgcagc gaaaatttcc
     1921 ggtgttggga aaagttttgc cggtgcgcat gattaatttc ttatcgcgct tcttcccacc
     1981 gctgtatatt tcgcacatca ccggcattcg atcgagcgcg acgggcaaag aaattgaggg
     2041 ttggtttgta gcgtgtccgt tcacaccgca gcggatgatg actttacccg cccgcatagt
     2101 gtatcggaag atcatcgcga cggcgaagaa ggcacaaagt ttaggcgcac aactggttgg
     2161 tttgggcgca tatacttccg tagtgggtga tggaggcgtt accatcggcc acatggttga
     2221 gatcccggta accactggtg acagttatac ggtggcgatt gcaattgaag cgctcaaaaa
     2281 agcggccaaa caaatggaga tcgatctttc aagtgccacc gcggcggttg tgggggccac
     2341 cggcacgatc ggggccgtga gtgccgagtt gttggcgcag gccgtgccgc agatcattct
     2401 aatcggtcgc aactccgatc gcttggggct agttaaagca cgctgtgaag ctgccggcgc
     2461 cgaagttcaa gtggcaactg atttgaacgc gctgcgtcaa gcagatctag tcctgaccgt
     2521 gacttcggcc atggattcga tcattgagcc cgaacatata aaatccggcg cggtggtgtg
     2581 tgatgtggcc cgaccgcgcg atgtgagcag gcaagttatc gagcagcgtg atgatgtgtt
     2641 agtgattgaa ggcggtatgg tggaagtgcc gggcgaagtt gacttccact ttaattttgg
     2701 ttttccgccg cgcatggcct acgcctgtat ggctgaaaca atggtgctgg cgttggatgg
     2761 ccgttatgaa agttattcct tgggcaagga tctcacgatc gaacaggtgc aggagattga
     2821 tcggattgcc cgatatcatg gctttacact gggcggtttt cgatcattcg agaacgccgt
     2881 cagcgcggaa caaatcgaac agatcaagct gaaggcccga tcgaaacggt tgggcttaaa
     2941 aacagttgca gcaatctctg ccgcctaaac gccgattggg tttaagctgg cacgctttgt
     3001 tgcagtcacg aggtgaagaa tgagtaaagg tcgcatccta gtcgtcgaag atgatttgga
     3061 catctctaac atgctgcgga tttatttcac ttcgcagggc tatgaagtca ccgtagcggg
     3121 ccggggcctt gaagcgctgg agcagacgcg caagcagctg cccagcgtga ttgtcttgga
     3181 tatcatgctg cccgacattg atgggtatga agtctgcgcc aagctgcggt ccgcgctgcg
     3241 caccagccac gttccaatca ttttcctgac gcaaaaagat gagcgcagcg atcgcattgc
     3301 aggtttggag ttgggcgcgg atgattacat cacaaagccc ttcgatattg aggagctgcg
     3361 cctgcgcgtg cagaatgcca tgcgccgcgc cgaacgcgag agcttgacca atccgacgac
     3421 aggtttgccc agcggcaagt tgatcgagga gcagctgcgc ggcttgatgc gccgcaaagg
     3481 ctggggactg ttgtatatcg gtttaaccga tctggatgcg ttcaacgacg tgtatggttt
     3541 cgtcgcgggc gatgaagtac tgcgttttac ggcgttgctg ttgggcgaga ctgacgacaa
     3601 agtgggcggc ccggatgatt tcatcgggca tgtgggcggc gatgatttta ttgtgattac
     3661 gacggatgaa aaagcgcagg cgttggcgga cgcggcgcac aaacgcttta gtcaagatat
     3721 tggctcgcat tattcattca aagatcgcga gcacggatac atcgttgtca aagatacaga
     3781 cgataaagag cgtcaggcgt cattgattaa gatgtgcatt ggcgtgatct tcgccaatac
     3841 ccaggagttt acggatattc gcgagatcac cgaagtttcc gcggaagcgc gacggcagat
     3901 gtgttcataa gaatgcctat cattcttagg gaacgtagca cccgacgcct agcggcccac
     3961 ctgagcgtac tgcgctaagg gtctgggcag aatctccagc tcaatcgcct gggttgttcg
     4021 taatgataga gattcttcac ttcgatcaga atgacattga gtaatggtaa tggtgatggt
     4081 gtgaaccagg atcagactcg acgagtgcaa atttcatttg gccgattggg tgtccggctg
     4141 tgggtaccat ttgtcatcgc ggcgatcgtg tccgtggcga ttgtcgcgct gttagtcgtt
     4201 gtcagcattg acaatcaaag cgcacaaatc agctcgacgc agcggagtgt ggcacaaagt
     4261 ttggttactc aggtcagcgg gtttgtggaa cgggttcgcg atgatcttaa aagattagtg
     4321 aatccagcca ctctgccgtc gttaagtcgg ctggtacttg atcaagtctt aagcaacgcc
     4381 attcgcgatc aggctgcctt ttcggatctt tctttggttg gattgaatgg tttcgaaatt
     4441 gcgcgagtcg cgcgtaatga taacacgatc attattttga ctgatcgatc aggctctgcg
     4501 gagtttcaaa ctgctgcggc cggtcgagag tttattgggc cagtccgaac gatcgagggt
     4561 gtgcccactg tcagaatcgg tgtacctatt ttcaatgacc gccaagatgt gatcgatgtg
     4621 gttgtcggtg aagttgatct gcagagctta tgtgatgtgg tgtgcactgt cagtttcgga
     4681 ctacaggggt atgcctattt aatcgacgat catggcaact tgatcgccta ccgcgatttc
     4741 aaatcggtgg tcagcccggt gacttatatg ccggcggcaa acgatgcgat tattaatcac
     4801 ggtcagttaa ccggcccgca gctatttgat acggggttgg cgtcccacgg cgaatcgact
     4861 ttagcttatt acgccccagt gctggttaca gattccagcc gatggtatat cgtccttgag
     4921 cagccaaccg ttgatgcctt cgcccaactc aatgagctga tcctgatcgg tatcggtcta
     4981 gtcctgattt cgttgggctc tatcgtggtg atgggcattt taatttcacg acgggtgatg
     5041 agaccgttgg ccatgttgcg acgcggtgct gcgcagcttg cccggggcga tctgcaccat
     5101 cgcatcactg gtgtgcagac cggtgatgaa ttgcaatttt tagctgcaga atttaatgag
     5161 atggcgacca agctgcaaga agcccagagc aattccgcca gtgtggcact tgagcggcaa
     5221 cagcaatttg aagtggtgca gcggcgcgtc aaagaaatgt ccacactgct tcaaaccgga
     5281 cgagcca
//
LOCUS       DASRTJ010000091         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_42938,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000091 DASRTJ010000000
VERSION     DASRTJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42938"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1290
                     /locus_tag="VFF70_01755"
     CDS             157..1290
                     /locus_tag="VFF70_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZY43446.1"
                     /translation="MCGMTPRERVLTTFAHKEPDRMPVDMFGTACCLMDPAYFALRDH
                     LGLTGDGRIFRKGQAISYYDERILERLEVDFRRVWLREPINWQPHVIDVETVTDEWGL
                     HLKRVGDTLNVINAPLEKATISDLAAYPWPDPYDPGRIAGLAEEAKHLRETTECAISA
                     RSPFYGIFEIAQRLRGAQQFLLDLAMDKKFATALINQLKEVTLGFFDAYLGAVGPYVD
                     MVEHSDDLGSQAGPLISPKTFKEIIAPARKELNDLIKRKAPNAKIFLHSDGSIFKMIP
                     QLIEIGVDILNPVEPDAAGNDAQNLKKTFGSELVFHGHLDTKGALRGSIEDVRAEVRR
                     VFDNMGSGGGYIMAPTNHLQIDVPPDHVVELYQYAHQYGQYRP"
     gene            1303..>1694
                     /locus_tag="VFF70_01760"
     CDS             1303..>1694
                     /locus_tag="VFF70_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZY43447.1"
                     /translation="MIDIHVHLWDWRDTPGFISEYMISRGYFGDQAGESFFNTPALLA
                     ALDEGAVDLGVLLPLRSENHNWRVSNEYVKQIVDEAQGRLIGFAGVSPLAYDATTELR
                     HAIETLNLRGLKLHPPMQMFDPGDSRCFP"
BASE COUNT          369 a          469 c          499 g          357 t
ORIGIN      
        1 gcctgaagtg aaagctgccg ccgatgtcat cgcgccgacg aatgatgcgg gcggcgtggc
       61 ctgggcactg cgcgagttga tcttgaagca aacgtcgtag agacgattga tcaatcaata
      121 atcacgcggg ggataaataa ttatggagga tcgaacatgt gcggcatgac cccgcgtgaa
      181 cgtgtactga caactttcgc ccacaaagaa ccggatcgga tgccggtcga tatgttcggc
      241 acggcgtgct gcttgatgga ccccgcctac tttgcactgc gcgatcatct cggactcacc
      301 ggcgatggcc gcatcttccg caagggtcaa gcgatctcgt attacgacga gcgtatactt
      361 gaaagactgg aagtcgattt ccgccgagtg tggctgcgtg agccgatcaa ttggcagccg
      421 cacgtgattg acgttgagac cgtcaccgac gagtggggtt tgcatctcaa gcgagttggc
      481 gatacgttga atgtgatcaa cgcaccgttg gagaaagcga cgatcagcga tttggctgct
      541 tatccctggc cggatccata cgatccgggt cgcattgccg gcttggctga agaagcgaag
      601 catctgcgcg agacgaccga gtgtgccatc tcagcccggt cgcccttcta cggcatcttc
      661 gagatcgcgc agcggctgcg gggggcacag cagtttctac ttgatctcgc tatggacaag
      721 aagtttgcca cagcattaat caaccaactt aaggaagtga cgctgggctt cttcgatgcc
      781 tacctgggcg cagtgggccc ctacgtcgat atggtcgagc acagcgatga cctcggctcg
      841 caagccgggc cgctgatctc acccaagacc ttcaaagaga tcatcgcccc ggcccgcaag
      901 gaactgaatg acttgatcaa acgcaaagcg cccaacgcca aaatctttct gcactccgac
      961 ggctccatct tcaagatgat cccgcagctg attgagatcg gcgttgacat cctcaatccg
     1021 gtcgagccgg atgcggccgg taatgatgcg cagaacttga agaaaacatt tggctcggaa
     1081 ctggtctttc atggacactt ggacaccaaa ggcgcgctgc gcggctcgat cgaggatgtg
     1141 cgcgctgaag ttcggcgcgt gttcgataac atggggtcgg gcggcggtta catcatggcc
     1201 ccgacgaatc atctgcagat cgatgttccg ccggatcacg tcgtcgaatt gtatcagtat
     1261 gcccatcagt acggtcagta tcggccctga aaggaacgat ccgtgatcga cattcacgtt
     1321 catctgtggg actggcgcga cacgcccggc tttatctcgg agtatatgat ctcgcgcggc
     1381 tacttcggcg atcaagcggg cgagtcgttc ttcaacacgc ccgcgctgct ggccgccttg
     1441 gatgagggcg cggtcgatct gggggtgttg ctgccgctgc gctcggaaaa tcacaactgg
     1501 cgtgtcagca acgaatacgt caaacaaatt gtcgatgagg cacagggtcg attaattggg
     1561 ttcgcaggcg tctcaccgct ggcgtatgat gcgacgacgg aactgcgtca cgcgatcgaa
     1621 acgctgaacc tgcgcggctt gaaacttcat ccaccgatgc agatgttcga tccgggtgac
     1681 tcgcgctgct tccc
//
LOCUS       DASRTJ010000092         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_191447,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000092 DASRTJ010000000
VERSION     DASRTJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191447"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1241)
                     /locus_tag="VFF70_01765"
     CDS             complement(147..1241)
                     /locus_tag="VFF70_01765"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZY43448.1"
                     /translation="MSIIIDAHEDIAWNVLCYGRDYFKGAQAIRQREAGGAVETATGQ
                     AMLGLPEWLAGGVAIVFGTIFTLPDRKKNDGLGLAYKTPNEAHVLGMRMLDIYHRMAD
                     EQPQIKLIKTQTDLDAVLKTWDGFDPMNPALGQSQRQIGIVMSMENGDPIRTPDEVKM
                     WYDRGLRAVGPAWAGTRYCGGTNEPGPLTDDGFKLLKVMHNLNMLLDLTHMDEQAYFQ
                     ALDRYDGPLLASHSNPRVFTQNTNRHLTDDMIKALIARDGVIGTVIFNKFLLPDWNTG
                     DPKEAATVKTVVERIDYVCQKAGDARHAAIGTDFDGGYGAASTPAGFDTVADLKIIGP
                     ALAEKGYSPANIDLILNGNWLRLLRRSLPA"
     gene            complement(1333..2079)
                     /locus_tag="VFF70_01770"
     CDS             complement(1333..2079)
                     /locus_tag="VFF70_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZY43449.1"
                     /translation="MSDQPREMVVIKNLVKRFGRLRAVDDVNLKVNQGQVLVIIGPSG
                     SGKSTVLRCINHLETPTSGEIWVDGIHVSHNHKDINAIRAEVGMVFQQFNLFPHLTAL
                     GNIMLAQKIVRKRDGEEARQMGLRQLERVGIPEKADEYPGQLSGGQQQRVAIARALAM
                     NPKIMLFDEPTSALDPEMIKEVLEVMLTLAQEGMTMVVVTHEMGFAKNAASEIIFMDH
                     GKIVESSSPAEFFKAPREERTRLFLSQILH"
     gene            complement(2335..3198)
                     /locus_tag="VFF70_01775"
     CDS             complement(2335..3198)
                     /locus_tag="VFF70_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZY43450.1"
                     /translation="MPASEPLSSNRSTQGQFIQQLSRLPWWLLFILFGGVFLFYKIST
                     DELYTTILSKLLQGVALTVFVAVVSYFVAIVIGLIAGLGRVSKNPIIFNIATLYVQII
                     RGVPILVQIFYVAFVLAPAIFTLLHNLGLSLVGLLGPDNFLATSSTQDLSMLARVMIA
                     LAIAYGGFEAETFRAGIESISKGQMEAARSLGMSYFHAMRYVIMPQAVRRVLPPLSND
                     FISMIKDSSLVSVLGVRDVTQEARLYAAASFRYPETYNTLAFVYLTMTILLSLGVKWL
                     ENRMGQEGRSG"
     gene            complement(3388..5010)
                     /locus_tag="VFF70_01780"
     CDS             complement(3388..5010)
                     /locus_tag="VFF70_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZY43451.1"
                     /translation="MKTFRIFSVLIVAMLIVSACAPAVTPTATSTPTTAVQPTKAPAS
                     NLPDLGGREVTIAIEDAYIPFNYVRLDTGKAEGWDYDALAEICKRLNCKPVFKEIGWD
                     GMITAVSQGQFDMAADGITITDDRAKVVDFSDGYIAIDQRVMVRKDTTLTSAANFGTD
                     SKLKLGTQKGTTNYDQAVKMVGESRVVAFDSFGDAVQALINKDVDGVVIDDTAGVGYV
                     GVNKEQIKLLPEKLVSQQLGFVFPKGKDLVKPFNVAIAALKADGTLDKLAAKWFATTP
                     IDPNAIGPGAYAPVLPDLAGKEVTVAIEDAYIPFNYIRLDNGQAEGWDYDAVAAICKL
                     LNCKPVFKEIGWDGMITAVSQGQFDMAADGITITDDRAKVVDFSDGYISIVQRIMIRK
                     DDAAITKADDFKTNTKLKLGTQKGTTNYDEAVTLVGEARVVAFDTFGDAVQALINKDV
                     AGVVIDDTAGVGYVGVNKDQLKLLPDSLVSQELGFVFPKGSALVEPFNMALEAMKADG
                     TLDQLAVKWFAATPIDSSLIGPGAYGTATPTP"
     gene            complement(5118..>5409)
                     /locus_tag="VFF70_01785"
     CDS             complement(5118..>5409)
                     /locus_tag="VFF70_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43452.1"
                     /translation="YKTKRGTVKFTLHNCPRYWTAEYHWGGWRAKYEVRLESFGEAWT
                     RMTIEIDLQPQSFLARVQSSATRSKTNRYLKSRLAAAAQKFYAALPEGSSLT"
BASE COUNT         1231 a         1525 c         1405 g         1248 t
ORIGIN      
        1 gccccttgag cattgacctg cgcagttcgc gtcgccagcc ctgaaccgac agcggctcat
       61 ttaattgaac gggccgtttt gatttagaaa acaaaatcgc tccacgagca aacgcttgtg
      121 gagcgattca tttaacgtca aagtttttac gccggcagcg atcgacgcag cagacgcagc
      181 cagttgccgt tgaggattag atcgatgttc gcaggtgaat aacctttttc ggccagcgca
      241 ggcccgatga tcttcaaatc agcgacagta tcaaagccag ccggtgtcga agccgcgcca
      301 tacccgccat cgaagtccgt gccgatggcg gcgtggcgcg catcgcccgc tttttggcag
      361 acgtaatcga tccgctcgac cacggtcttc accgtggcag cctctttagg atcgccggtg
      421 ttccaatcgg gcagcagaaa tttattaaag atgactgtgc cgatcacacc atcgcgcgcg
      481 atcaacgcct tgatcatgtc atcggttaaa tgccggttcg tattctgagt gaacacgcgc
      541 gggttggaat ggctggcgag cagcgggccg tcatatcgat ccagcgcctg gaagtacgcc
      601 tgttcatcca tgtgcgtcag atcgagcagc atgttgaggt tgtgcatgac cttcaataac
      661 ttgaagccat catccgtaag cggcccaggt tcatttgtac cgccgcagta gcgagtgcca
      721 gcccaggcgg gtccaaccgc tcgcaggccg cggtcatacc acatcttcac ttcatccggc
      781 gtgcgaatgg gatcgccatt ttccatcgac atgacgatgc cgatctgtcg ctgactttga
      841 cctagtgcgg gattcatcgg atcgaagccg tcccacgttt tcaataccgc gtcgagatca
      901 gtttgagttt tgatcaattt gatctgtggc tgctcatcgg ccattcgatg gtagatatcc
      961 agcatgcgca tgcctaacac gtgcgcttca ttcggcgttt tgtaagcgag cccgaggcca
     1021 tcgttcttct tgcgatcagg cagagtaaag atcgtgccga acacaatcgc cacaccaccg
     1081 gccagccact cgggcagacc caacatcgct tgaccggtag ccgtctccac cgccccaccc
     1141 gcctcgcgct gacggatcgc ttgcgcgccc ttgaaataat ctcgaccata acacagcacg
     1201 ttccaggcaa tgtcttcgtg ggcatcgata atgattgaca ctggattctc cgtaaaagga
     1261 tattgaagcc agatatcaga aggcaggata taaaactttc tcttctggca tctgaattct
     1321 acattctgaa ttctaatgca ggatctgact caagaacaag cgggtgcgtt cttctctagg
     1381 cgctttgaag aattcggcgg gtgagctgct ctccacgatc ttgccgtgat ccatgaagat
     1441 gatttcgctg gcggcatttt tagcaaagcc catttcgtgc gtcacgacga ccatcgtcat
     1501 accttcctga gctaacgtca gcatgacttc taaaacttct ttgatcattt ccggatcaag
     1561 cgccgaggtc ggttcatcga acaacatgat cttgggattc atcgccagcg cgcgcgcgat
     1621 ggcgacccgc tgctgctgcc cgcccgagag ttggcccgga tattcatcgg ctttttcggg
     1681 aatgcccacc cgctccaact gtcgcagacc catctgccgc gcttcttccc catcgcgctt
     1741 gcgcacaatc ttctgcgcca gcataatatt tccaagagcg gtcagatgcg ggaataaatt
     1801 gaattgttga aagaccatcc ccacttcggc ccgaatggcg ttgatgtctt tatgattgtg
     1861 tgacacatga ataccatcca cccagatctc gccactcgtt ggcgtttcca gatgattgat
     1921 gcagcgcagc acagtcgatt tgccggagcc gctcggcccg atgatgacca gcacctggcc
     1981 ctgattgact ttcaagttca catcatcgac ggcgcgcaag cggccaaatc gcttgaccag
     2041 attcttgatg acgaccattt ctctgggttg atcgctcata aattttccaa tttcagccgc
     2101 agcctataag catgtgactg gtcattaccc gccaccagtg ggcgcagtag atgatcgagc
     2161 ctggcatggg ttaaacgata tcgataaaaa ctgcaaaacg ttgagcgcgt aaaccgatcg
     2221 gcttagttga gcaaacccga tcagttatcg ggctgatcga tcaattcaag acgcccgatc
     2281 atcgcgccaa gcatgttgaa tcgatcgaaa taaatatcaa acgggctgat aagcctaacc
     2341 gctgcggccc tcctgtccca tccgattctc cagccacttc acgccgagcg acaataagat
     2401 cgtcatcgtg agatagacaa acgccagcgt gttgtacgtt tcaggataac ggaatgaagc
     2461 cgccgcatac aagcgagctt cttgggtcac atcccgcaca cccaagaccg agacgagaga
     2521 tgaatctttg atcatcgaga tgaagtcatt gctcagcggc ggcagcacgc gccgcacggc
     2581 ttgcggcatg atcacatagc gcatcgcatg aaagtaactc atgcccagcg aacgcgccgc
     2641 ttccatttgg cctttgctga tcgattcgat acccgctcga aaggtttccg cttcaaagcc
     2701 gccgtacgcg atcgccaacg cgatcatcac gcgggccagc atgcttaaat cttgtgtgct
     2761 gctggttgcc aggaaattat ccggtcccaa taggccaacc agcgataaac ctaagttgtg
     2821 cagtagagta aagatggccg gagccagcac gaacgcgaca tagaaaattt gaaccagaat
     2881 cggcacaccg cgaatgattt gaacgtagag tgtagcgata ttgaagatga tcggattttt
     2941 ggaaacacgc cccaagcccg cgattaagcc gatcacaatg gcgacgaaat aggagaccac
     3001 tgccacgaag acggtcaagg ctacgccctg cagcagcttg cttaagatgg tggtatacag
     3061 ctcatccgtg ctgatcttgt agaagaggaa tacgccgccg aacaaaataa acaacagcca
     3121 ccatggcagg cgcgacagtt gttgaataaa ctgtccctgg gtcgatcgat tgctgctaag
     3181 cggctcggaa gcaggcatgg tcgctacctt gctggctgag gtgttggggc atattgtatc
     3241 tgttgatggt ggaaagcaca aacgaagcag atgtgttcgc gtaccaatcg atcagcagat
     3301 acaaaatttc cggctctcga atattcgaga gccggaatgt gaagtgcgag cacctggcga
     3361 cagtttcagc acaaggattt agttttgcta cggcgtaggc gtcgcagtgc cgtacgcgcc
     3421 ggggccgatc agactggaat cgatcggcgt agcggcgaac cacttgaccg ccagttgatc
     3481 gagggtgccg tcggctttca tcgcctcaag cgccatattg aatggctcaa ccagggccga
     3541 tcccttgggg aagacaaagc ccaattcctg gctgaccaaa ctatcgggca gcagcttgag
     3601 ctgatctttg ttcacgccga cgtaaccgac accggcggta tcgtcgatca cgaccccagc
     3661 gacgtctttg ttgatcagag cttgcaccgc atcgccgaag gtatcgaacg cgacgacgcg
     3721 cgcttcgccg accaacgtta cggcttcatc atagttggtc gtgcctttct gcgtgccgag
     3781 tttcaatttt gtattcgtct tgaagtcatc ggctttggtg atggcggcgt catctttgcg
     3841 gatcataatg cgctgcacga tcgagatgta gccgtccgag aaatcaacca ccttggctcg
     3901 atcgtcggtg atggtgatcc cgtcggcggc catgtcgaac tggccctgcg acacggccgt
     3961 gatcatgccg tcccaaccga tctctttgaa gaccggcttg cagttcaata atttgcagat
     4021 ggccgcaacc gcgtcgtagt cccagccttc ggcctggccg ttatcaagac gaatgtaatt
     4081 gaagggaata taagcatctt caatcgcgac cgtcacttct ttaccggcga gatcgggcag
     4141 gacaggcgca taagcgccgg ggccgatcgc gtttggatcg atcggcgtgg tggcaaacca
     4201 cttggccgca agtttatcca gtgtgccatc ggctttcagc gctgcaatgg cgacattgaa
     4261 gggttttact aaatctttgc ccttcgggaa gacgaagccc aattgctggc tgaccagttt
     4321 ttccggcagc aacttgatct gttctttatt gacgcccacg taacccacgc cggcggtgtc
     4381 gtcgatcacc acgccatcga catctttgtt gatcaaggct tgcacggcat cgccaaaact
     4441 atcgaacgcg acgacgcggc tttcgccgac catcttgact gcctgatcat agttggtcgt
     4501 gcctttctgt gtaccgagtt tcaacttact atccgtcccg aagttagccg cactggtcag
     4561 cgtggtgtcc ttgcgaacca tcacccgctg atcgatcgcg atgtagccgt ccgagaaatc
     4621 aacgactttg gcacgatcat cggtaatggt gatgccgtcc gcggccatgt cgaactggcc
     4681 ttgagagact gctgtgatca tgccgtccca accgatttct ttgaagaccg gcttgcaatt
     4741 caagcgcttg caaatttcgg ccagcgcatc gtagtcccag ccttccgcct tgccagtgtc
     4801 caagcgcaca tagttgaacg gaatgtaggc atcctcgatc gcgatggtca cttctcttcc
     4861 gcccaagtcc ggcaggttgg atgccggagc tttagtcggc tgcaccgccg tcgtcggcgt
     4921 actggtggcc gtgggcgtca cggctggcgc acaggcgctg acgatcagca tagcgactat
     4981 caacacacta aaaattcgaa acgtcttcat ttcttctctc ctctttgaaa gttttgggat
     5041 gctgcagcga cacttgcggt ttcgaaccgc aagcgacagc acactcaatt gaaacgctat
     5101 tctagttcga tgcggctcta tgtcaaactc gatccctcag gcaaggccgc ataaaatttc
     5161 tgcgcggcag cggccaggcg ggacttcaaa taccgattgg ttttcgagcg cgtcgcggaa
     5221 ctctgcacgc gcgccaagaa cgactgcggc tgcagatcga tctcgatcgt catacgcgtc
     5281 caggcttcgc cgaatgattc caggcgaact tcatactttg cgcgccagcc gccccagtga
     5341 tattcagctg tccaatagcg cggacaattg tgcagggtga atttgacggt gccgcgcttc
     5401 gttttataa
//
LOCUS       DASRTJ010000093         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_307975,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000093 DASRTJ010000000
VERSION     DASRTJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307975"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFF70_01790"
     CDS             complement(<1..1056)
                     /locus_tag="VFF70_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase"
                     /protein_id="HZY43453.1"
                     /translation="MTTQLLLGNDAIALGMVQNNCRVVTAYPGTPSSEILAGVVKFKK
                     QLNREIYTEWSANEKVAFEIALAASWSGLRAATTMKQVGLNVAADPLFSAAYTGVVGG
                     FVIVTCDDPGPQSSQTEQDSRLMALTAKVPVFDPATPAEALAMMQAAFELSERHRIPV
                     IFRPTTRVCHAVQSVDVNGSTPNPLLAAKTGFKKDPQRWAATPAFRLKLHHELNAKLQ
                     AIEAEFETSPLNFINRSDAETDRKLGLIAGGVTYQVALQALAELGVRVPVMKIGTPYP
                     LPRKKVAEFIAQFDQVLVLEEPDACIELQIADRTHVTGRLDYAVPNAGELSPEVISAV
                     LATVLKKVGVTVPPPPDD"
     gene            complement(1171..2544)
                     /locus_tag="VFF70_01795"
     CDS             complement(1171..2544)
                     /locus_tag="VFF70_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZY43454.1"
                     /translation="MSDQHKFLLDESKLPKAWYNINADMPVPPQPVLHPGTLEPVTPD
                     FLSVLFPMNIIMQEISTDRWVEIPEPVREIYKLWRPTPMMRAVRLEKMLDTPAHIYYK
                     YEGVSPVGSHKPNTAVPQAFYNKEAGTKALTTETGAGQWGSALAMACNFFGIDLEIYM
                     VKVSYNQKPYRRIIMQSFGAQVYASPSDRTNVGRSVLAEHPDSPGSLGMAISEAVEVA
                     ATSGGTKKYSLGSVLGHVLMHQTVVGLEALQQMEMAGEYPDMVIGCAGGGSNFAGFTY
                     PFLHQNFTAGRKTRVIAVEPASCPSLTKGKYTFDFGDSAAMAPVVKMYTLGHTFIPPS
                     IHAGGLRYHGMSPSVSALVDHGDIEARAVKQLETFEAALMFMKSEGIIPAPESAHAVR
                     VAIDEALKCKQDGTKKVIAFNLSGHGHFDMMAYDAYNQGQLQDYEYPVEMMAEAMTHL
                     PKVSIPA"
     gene            complement(2663..2935)
                     /locus_tag="VFF70_01800"
     CDS             complement(2663..2935)
                     /locus_tag="VFF70_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43455.1"
                     /translation="MAEIDHMVAPGLNKPPDPKLVAEGWERRFMIDKRRVAEFTELYQ
                     SMGYDVRAETVRDEEVDPECGDCALVMYQIFVTLYTRRSGSSAQSG"
     gene            complement(2938..3795)
                     /locus_tag="VFF70_01805"
     CDS             complement(2938..3795)
                     /locus_tag="VFF70_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZY43456.1"
                     /translation="MIYTAGIDVGSTYTKAVIMRDDDQIAGRGLINTGFKLLEATTRA
                     LQLALDQAGLRQDNLAYIATTGYGRFQVPFRDLNITDLTASASGARYFFPKTQTILDV
                     GGQTMKASRLQEDGRVKSFRLNDKCAAGTGAFLEKTARYMGYSTTEIGPLAATSKEVI
                     AISGVCAVFAESEVINHLSQGSSPADIMYGSIVSLVDRSVQLMKRVQMEPEFTLVGGI
                     LRYESMVKIIREKLGANVNVPEGDLVQFTAALGAALLARRRLNKLSEQGAVIDRGQVE
                     LSDQLIAGT"
     gene            complement(3933..4751)
                     /locus_tag="VFF70_01810"
     CDS             complement(3933..4751)
                     /locus_tag="VFF70_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZY43457.1"
                     /translation="MAYSAGVDVGSTQTKAVILNEDRQIVSRALIDTGANVVKAAENA
                     YVEALKNGNIREEEVEYVVGTGYGRYKVTFGDTQITEISCHGRGAVHMFPNTRTVVDM
                     GGQDTKAIRVSPTGEVIDFCMNDKCAAGTGRFLGAAAAALEIPLDELGQTALRAEKPV
                     KISTTCTVFAESEVVSWLGKGKKLEDILLGVHQSIVSRSMGLMRRVGIEPEVSFTGGV
                     AKNIAVVAELNKAVGFKVNVSEDSHYMGALGAALFAMDRIMASRVPSSQLEKAA"
     gene            complement(4783..6087)
                     /locus_tag="VFF70_01815"
     CDS             complement(4783..6087)
                     /locus_tag="VFF70_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZY43458.1"
                     /translation="MTDSLATATDSLVGRGAKDGAQMFRDWFQTLTTAAERNEKAAYV
                     FVMGSLAELLRVFDFHTVFPEINALQTAVRRVSAEYLNEAEDYGYSPDICGYVKADVA
                     MQLRGGQHPMGRIPRPSLAVYTNACNTYIKWAEIWERLYHIPIFTLDVPGTRAAGGQT
                     WPGQRDFENDRRYVAAQIKELITVCEEVSGKKFDIDRLRETLANANVMSSAWKRVLKL
                     NESTPAVFNALTDGTVYLGVSNGFRGTAAGAKYFTDLVEEMEYKAAHGLGTLTEEKYR
                     LLFVGVPCYPIFRRFNEMFSEWGGAFISSTYLWFASGGTNMGFQYDLDHPLESLAEGV
                     LISVRDAMDSMFFQDQVLIDMVKPYHIDGVVYHPIKSCRTTSTGLADNRRSLMSKTDV
                     SSLFIESDMMDKRVVSEAQLKNRIDAFFEGLATRKQMIANRG"
     gene            complement(6152..>6632)
                     /locus_tag="VFF70_01820"
     CDS             complement(6152..>6632)
                     /locus_tag="VFF70_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HZY43459.1"
                     /translation="IESRSAKPQDRIRVVFEGGFCEQPPLDLLHNISQSIYVVDDDLL
                     IGLRWLTEDVTLNGDPLYNLAEAYLEKSSYSPVQHDLRKPKEKMLLERIKNSHASAAI
                     ITAAKMCEPGLDEQVAYVKALDEAGIPYFVSEFEESMTSFDHLQIQLETFVENLLFA"
BASE COUNT         1362 a         1968 c         1768 g         1534 t
ORIGIN      
        1 atcatcgggc ggcggcggaa ctgtcacgcc gaccttcttc aaaacggtgg ccaggacggc
       61 tgaaataact tcgggcgata actcaccggc gttgggcacc gcataatcca ggcggcctgt
      121 cacatgcgtc cgatcggcga tctgcaactc gatgcacgcg tccggctctt cgaggacaag
      181 tacctgatcg aactgcgcga tgaactccgc cacttttttg cgaggcagcg gatacggtgt
      241 gccgattttc atcacgggca ccctgacgcc taattccgct aaagcttgca aggcaacttg
      301 ataagtcacg ccgcccgcga tgaggcccaa cttccgatcg gtctcggcgt ccgaacggtt
      361 gatgaagttc agcggtgatg tttcaaattc ggcttcgata gcttgcagct tggcgttgag
      421 ttcgtgatga agtttcaatc gaaatgcggg cgtggcggcc cagcgctgcg gatctttttt
      481 gaagccggtc ttggcggcca acaacggatt cggcgttgaa ccgttgacat cgacactctg
      541 cacggcgtga cacacgcgcg tcgtcggccg aaagataacc ggaatgcggt gccgctctga
      601 aagttcgaag gccgcttgca tcatcgccag cgcttcggcg ggcgtcgctg gatcgaatac
      661 ggggactttc gcggtcagcg ccatcaagcg tgagtcttgt tcggtctgcg aagattgcgg
      721 gccgggatca tcgcatgtga cgatgacgaa gccgccgacc acaccggtgt aagcagcgct
      781 gaataatgga tcggccgcca cgttcaaccc cacttgcttc atcgtcgtgg ccgcgcgcag
      841 tccgctccaa ctggcggcta atgcgatctc aaatgccacc ttttcattcg cactccactc
      901 ggtataaatc tcgcggttta attgcttctt gaatttcacc acgcccgcca gaatttctga
      961 actgggcgtg cccggataag ctgtgacgac gcggcagttg ttctgaacca tgccgagggc
     1021 gatcgcgtca ttgccgagga gcagttgagt cgtcatgtca ttttctcaat ataaattacg
     1081 gacgaatttt gcgtggtgcg tgttgcgtac tcaacttctt acgcaccacg caccacgctt
     1141 tactcgttac tcgttacttg ttaatcatcc tcacgccgga atcgaaacct tcggcagatg
     1201 cgtcatagct tccgccatca tctcgaccgg atattcatag tcttgcaact ggccttgatt
     1261 ataagcatcg taggccatca tatcgaagtg gccgtggccg gacagattga aggcgatcac
     1321 tttcttggtg ccatcctgct tgcacttgag cgcttcgtcg atcgccacgc ggaccgcgtg
     1381 cgccgactcc ggcgcaggga taatgccctc ggacttcatg aacatcaaag ctgcttcaaa
     1441 cgtctcgagc tgcttcacgg cgcgcgcttc gatatcgccg tgatcgacta gcgcgctgac
     1501 actcggcgac ataccgtgat agcgcaggcc gcccgcatga atgcttggcg ggataaacgt
     1561 atggcccaac gtatacatct tcacgaccgg agccatcgcc gccgagtcgc caaagtcgaa
     1621 tgtgtatttg cccttcgtca gactcgggca ggacgctggc tcgacggcga tcacgcgcgt
     1681 cttccggccc gccgtgaagt tctgatgcag gaacggataa gtgaagcccg cgaagttcga
     1741 tccaccgccc gcgcagccga tcaccatatc gggatactcg ccggccatct ccatttgttg
     1801 cagtgcctca agaccgacga ccgtttgatg catcagcaca tgacccagca ccgagccaag
     1861 gctgtacttc ttcgtgccgc cactggtcgc cgccacttca actgcttccg agatcgccat
     1921 accgagcgag ccgggcgaat caggatgttc ggccaacacc gatcggccaa cattcgtccg
     1981 atcggacggg ctggcataca cctgcgcgcc aaagctctgc atgatgatgc gtcgatacgg
     2041 cttctggttg tatgacactt tcaccatgta gatttccaga tcgattccaa aaaagttgca
     2101 agccatcgcc agcgccgaac cccactggcc tgcgcccgtc tcggtggtca gcgccttcgt
     2161 gcccgcttcc ttattgtaga aagcctgcgg cacagcggta ttcggtttgt gcgaaccgac
     2221 cggcgacacg ccttcatact tgtaataaat gtgcgcgggc gtatccagca ttttctccag
     2281 ccgcacggcg cgcatcatcg gcgtgggccg ccacaacttg taaatctcgc gcaccggctc
     2341 tggaatttca acccagcgat cggtgctgat ctcttgcatg atgatattca ttggaaacag
     2401 cacgctcaga aaatcgggcg tcaccggctc cagcgtgcct ggatgcagca ccggctgcgg
     2461 cggcaccggc atatcggcgt tgatgttgta ccacgccttg ggcagcttac tttcatccaa
     2521 aagaaatttg tgttggtcgc tcatcgatac tctcctgtct gtagtttgga aatgcaagct
     2581 agattcgatc gagatgctag cgggctattc ttcttatcga ttcgatcgcg gctcctttcg
     2641 tagctgagat ttcaaatctc aattaacccg attgtgctga gctgcccgat cggcgcgtgt
     2701 ataacgtgac aaagatctga tacatcacca gtgcgcaatc gccgcactcc ggatcaacct
     2761 cttcatcccg aaccgtttca gcgcgcacgt catagcccat cgattgatac aactcggtaa
     2821 attcagccac gcgccgcttg tcgatcatga accgccgttc ccagccttcc gccaccaact
     2881 tcggatcggg cggcttattc aagcccggcg cgaccatgtg atcaatctca gccatgctta
     2941 agtcccggcg atgagttgat ctgacaactc gacttgaccg cgatcgatca ctgcgccttg
     3001 ctcgctcaat ttgttcaagc ggcgacgcgc caataaggcc gcgcccagcg ccgccgtgaa
     3061 ttgcaccagg tcgccttccg gcacattgac attggcaccc aatttttctc gaatgatctt
     3121 gaccatcgat tcgtaacgca ggatgccgcc gaccagcgtg aactccggct ccatctgcac
     3181 gcgcttcatc agctgcaccg atcgatccac cagcgacacg atcgaaccgt acatgatgtc
     3241 ggccggcgaa ctaccttgcg agagatgatt gatcacttcg gattcggcaa agaccgcaca
     3301 cacgccggag attgcgatca cttctttcga tgtcgccgcc agcggtccga tctcagtggt
     3361 gctgtaaccc atgtagcgcg ccgttttttc caggaacgcg ccggtgcctg ccgcgcattt
     3421 atcattcaag cggaaagatt tgacgcggcc atcttcttgc aagcggctgg ccttcatcgt
     3481 ttgtccgccc acgtccagga tcgtctgcgt tttcggaaag aagtaacgcg caccggacgc
     3541 cgaagcagtc agatcggtaa tgttcagatc gcggaacgga acttggaagc ggccgtagcc
     3601 ggtcgtggcg atataagcca aattgtcttg tcgcagaccc gcctgatcga gcgccaactg
     3661 caaggcgcgc gttgtggctt cgagcagttt gaagccggtg ttgatcagtc cgcgtccggc
     3721 gatctgatcg tcgtcgcgca tgatcacggc tttggtgtaa gttgaaccca cgtcaatgcc
     3781 tgcggtataa atcatgtttg tcctccggta actaaccttt ctctcataga ctttcgcttg
     3841 tcatttcgaa cgaagtgaga aatctctgcc tcgattagcg gaccatgctc atcagcgtcg
     3901 agatttctcg caaaaaacgc tcgaaatgac ggctaagcag ctttctctaa ttgactcgac
     3961 ggcacacggc tggccatgat gcgatccatt gcaaacaagg ccgcgcctaa cgcgcccata
     4021 tagtgcgagt cctcactcac attcactttg aagcctacgg ctttgttcag ctctgcaacg
     4081 acggcgatgt tcttagcgac gccgccggtg aagctcactt ccggttcaat accgacgcgg
     4141 cgcattaagc ccatcgagcg tgacacgatc gattgatgca cacccaatag aatgtcttcc
     4201 agcttcttgc ctttgcccag ccacgacacc acttcggatt cggcaaagac cgtgcaggtc
     4261 gtgctgatct tcacgggttt ctccgcgcgc aaggcagtct gacctaattc gtccagcgga
     4321 atttccagcg cggcagccgc cgcgcccaga aaacgtcccg tgcccgccgc gcatttatcg
     4381 ttcatgcaga agtcaatcac ttcgcctgtc ggactgacgc gaatggcttt agtatcctgc
     4441 ccgcccatgt cgacgacggt gcgcgtgttg gggaacatat gcaccgcgcc gcgcccgtgg
     4501 caagagatct cggtgatctg cgtgtcgcca aacgtgactt tgtagcggcc atagcctgtg
     4561 ccgacgacat actccacttc ttcttcgcgg atgttgccgt tcttcagcgc ttccacgtaa
     4621 gcgttctcgg cggctttcac tacattagcg cccgtatcga tcaacgcccg gctgacgatc
     4681 tgtcgatctt cgttgaggat gacggctttg gtttgggttg aacctacatc taccccggct
     4741 gaataggcca tgttggtctc ctggttggtg tgtcgcgtat tgctatccgc gattcgcgat
     4801 catttgcttc cgcgtggcca gtccctcaaa gaacgcgtcg atgcgatttt tcaactgtgc
     4861 ttcagagacc acgcgcttat ccatcatgtc cgattcgatg aacaggctgc tgacatcggt
     4921 cttggacatc agtgagcgcc gattatccgc caacccggtc gaggtggtgc ggcaggattt
     4981 gatcgggtga tagaccacgc catcgatgtg ataaggtttc accatatcga tcagcacctg
     5041 atcctgaaag aacatgctgt ccatggcatc gcgcacgctg atcaatacgc cttcggccag
     5101 actttccagc ggatgatcca gatcgtattg gaagcccata ttcgtgccgc cagacgcgaa
     5161 ccacaaataa gtggaactga tgaacgcgcc gccccattca gaaaacattt cattgaagcg
     5221 ccggaagatg ggatagcacg gcacgccgac gaacagcaga cgatattttt cttccgtcag
     5281 tgtgccaagt ccgtgcgccg ctttgtattc catctcttcg acgagatcgg tgaaatactt
     5341 tgcgccggcc gcggtgccgc ggaagccatt cgacacgcca aggtaaactg tgccatcggt
     5401 cagcgcgtta aagacggcgg gcgtgctttc gttcaatttc agcacgcgct tccacgcgct
     5461 gctcatgacg ttggcattcg ccagcgtttc gcgcagccga tcgatgtcga acttcttgcc
     5521 gctgacttct tcgcacacgg tgatcagttc tttgatctgc gcggccacgt accgccgatc
     5581 gttctcgaag tcccgctggc ccggccacgt ttggccgccg gccgcgcgtg tacccggcac
     5641 atcgagcgta aagatgggaa tgtgatacag ccgctcccag atctcggccc acttgatgta
     5701 ggtattgcag gcgttggtgt acacggccag tgacgggcgc ggaatgcggc ccatgggatg
     5761 ttggccgccg cgcagctgca tcgccacatc ggctttaacg tagccacaga tatcgggcga
     5821 gtagccgtag tcttcggctt cgttcagata ttcggccgac acgcgccgca cagcggtctg
     5881 caaggcgttg atctccggaa agaccgtgtg aaaatcaaac acgcgcaaaa gctcggctaa
     5941 gctgcccatg acaaaaacat aagcggcctt ctcgttacgc tcggcggccg tggtgagcgt
     6001 ttgaaaccag tcgcggaaca tttgcgcgcc gtctttagca ccgcggccta caaggctgtc
     6061 tgttgctgtt gctaatgaat cggtcatgtt agtctcctcc gtattgcgta ttacctggtg
     6121 cgttgactcg ttacttgtta ctcgttatcc gtcacgcaaa cagtagattc tctacaaacg
     6181 tctctagctg aatctgcaaa tgatcgaagc tggtcatgct ttcttcaaat tcgctgacga
     6241 agtaaggaat gcccgcttcg tcaagcgctt taacgtaggc gacttgttcg tcgaggcccg
     6301 gctcgcacat tttggcggcg gtgatgatcg ccgcggaagc gtgggagttc ttgatgcgtt
     6361 ccagcaacat tttttctttc ggcttgcgca gatcgtgctg caccgggctg tacgatgatt
     6421 tttccagata ggcttccgcc aagttgtaca ggggatcgcc gttcaacgtc acatcttcgg
     6481 tcaaccagcg cagcccgatc agcagatcgt cgtcgaccac gtagatcgac tgcgagatgt
     6541 tgtgcagcag atcgagcgga ggctgctcgc agaagccgcc ttcaaacacg acgcggatgc
     6601 gatcctgcgg tttggcggag cgactctcga tc
//
LOCUS       DASRTJ010000094         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_308055,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000094 DASRTJ010000000
VERSION     DASRTJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308055"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFF70_01825"
     CDS             complement(<1..576)
                     /locus_tag="VFF70_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43460.1"
                     /translation="MSIPKSEAIHPKSEIKTIVVLFVVLRLTILLLYTPQGLLNAYTD
                     TNYYYRTAQLSDQGYYPFVNMWYEYPPIATYISQGVYSLTRSLPANGTLDIRYQVYSR
                     LLSGLFLIFETGVLILIHRIAAKCWETVTADRLGWIYSVLSVPLFFWNASQNSVVVFF
                     ALWSIEAFLDSHQIRSAIALGLGIATKFTPFF"
     gene            653..1816
                     /locus_tag="VFF70_01830"
     CDS             653..1816
                     /locus_tag="VFF70_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZY43461.1"
                     /translation="MKNQRTIYLDHAATTPVDPRVIEAMQPYWTDHFGNTSSIHAIGR
                     EAQQGLENARRDVAEILNCDPHEIIFTGCGTESDNLALRGVAWAARKSGRGNHIITTK
                     VEHHAVEVTAEQLHELNDFDVTFVDVDEYGRVDPDSIGRAIRSDTVLISVMYANNEVG
                     TLQPLAEIGSVARSKGIAFHTDAVQAGTSMNLKVDDLKVDLLALSAHKFYGPKGVGVL
                     YARSSVELISAITGGGHERGRRPGTVNVAEIVGLATALKLAQADRATENARVRSLRDR
                     LIAGVLDRIPDTRLTGHPINRLPNNASFAIRGVIGENVLMALDLEGICVSTGSACTSA
                     EVEPSFVLTAMGLPYDWAIGSLRMTLGHLTHAEDIDRVLSVLPQVVERLRVHA"
     gene            1885..>2422
                     /locus_tag="VFF70_01835"
     CDS             1885..>2422
                     /locus_tag="VFF70_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZY43462.1"
                     /translation="MSDQNSKTRVVVAMSGGVDSSVAAALLVEQGYDVVGIMLKLWSE
                     GESNRVNRCCTPADTEAARSMANQLGIPFYLINIADSFKSIVVDYFVNEYAAGRTPNP
                     CLECNRHIRFELLLNKALGLGARYLATGHYARVRQTDGQYQLLRGIDPKKDQSYVLSV
                     LGQPELSHALWPLGEMTKP"
BASE COUNT          560 a          599 c          723 g          540 t
ORIGIN      
        1 gaagaagggg gtgaacttgg tggcgatgcc cagtcccagc gcaatggccg atcgaatttg
       61 atgcgaatcg aggaaggcct cgatcgacca caaggcgaaa aacacgacca ccgaattttg
      121 gctggcattc caaaagaaca gcggcacgct caagactgaa tagatccagc ctaatcgatc
      181 ggcggttacg gtctcccagc atttggccgc gatccgatgg atcaagatca gcacaccggt
      241 ttcaaagatc aagaagaggc cgctcaataa gcgcgaatag acttgataac gtatatcgag
      301 cgtgccgttg gcaggcaggc tgcgcgtgag tgaatagacg ccttgcgaaa tataagttgc
      361 gatcggcgga tattcgtacc acatgttgac gaacggatag tagccttgat cggatagctg
      421 cgcggtgcga tagtaatagt tggtgtcggt gtaggcgttg agcaaacctt gcggcgtgta
      481 aagcagcagg attgtcagac gcagcacgac gaacaaaacg acgatcgttt tgatctctga
      541 tttcggatgg atggcctctg atttaggaat actcatgact cgttactcag gttacgtttt
      601 cattgaccgt gtaccctcca catgttacaa tcttttgcac aagagagcaa tgatgaagaa
      661 tcaacgaacc atttaccttg atcatgccgc gaccacaccg gtcgatccgc gcgtcatcga
      721 agcgatgcag ccgtattgga ctgatcactt cggcaatacc tcgtcgatcc atgccattgg
      781 gcgcgaggct caacagggcc tggagaatgc gcgccgggat gtcgctgaga ttctaaactg
      841 cgatccgcat gaaattattt ttaccggctg cggcaccgaa agtgacaatc ttgcactgcg
      901 cggcgtggct tgggcggcgc gcaaaagtgg tcgcggcaat cacatcatca cgacgaaggt
      961 tgagcatcac gcggtcgaag tgacggccga gcagctgcat gagctcaatg acttcgatgt
     1021 gacatttgtc gatgtcgatg aatatggccg cgtcgatccg gactcgatcg ggcgggcgat
     1081 ccgatcggac acggtgttga tcagcgtgat gtatgccaac aacgaagtcg gcacgctgca
     1141 acccttggcc gagatcggat cggtcgcgcg atcgaaaggc atcgcgtttc atactgatgc
     1201 ggtgcaagcc gggacctcga tgaatttgaa agtcgatgat ctcaaagtcg atcttctggc
     1261 gttatcggcg cacaaatttt atgggccgaa aggcgtcggc gtgttgtatg cccgatcgag
     1321 cgtcgaactc atttcggcca ttaccggcgg cgggcatgaa cgagggcggc ggcccggcac
     1381 ggtgaatgta gcggagattg tcggactggc aacggctttg aagttggctc aggccgatcg
     1441 agcaacagaa aatgcccgcg tgcgatcgct gcgcgatcga ttgatcgccg gtgtgctcga
     1501 tcgaattcct gacacgcgct tgaccggcca tccgatcaat cgattgccga acaatgcatc
     1561 gttcgccatt cgcggcgtta tcggcgaaaa cgtcttaatg gcgctcgatc tggaaggcat
     1621 ttgcgtcagc accggatcgg cctgcacgag cgctgaggtc gagccgtcat ttgtgttgac
     1681 cgcgatgggc ttgccgtatg actgggcgat cggttcgctg cgtatgacat tagggcacct
     1741 gacgcacgct gaagatattg atcgcgtatt aagtgtgctg ccgcaagttg tcgagcgatt
     1801 gagagtgcat gcatgagcga agtgcgcatt tactatttac tttctactaa tgagtgaatc
     1861 gtaatgagtg attgatgata tgtgatgagt gatcagaatt ccaagaccag agttgtggtt
     1921 gctatgagcg gcggcgtcga tagttccgtc gcggcggcgc tgctggttga gcagggctac
     1981 gacgtggtcg gcatcatgct gaagttgtgg agcgagggcg agagcaaccg cgtcaatcgc
     2041 tgctgcacgc ccgccgacac tgaagccgcc cgatcgatgg cgaatcaact cggcatcccg
     2101 ttttatctga tcaacatcgc cgattcgttc aaatcgatcg tcgtcgatta ctttgtcaat
     2161 gaatatgcgg cgggccgcac cccgaacccg tgtttggaat gtaatcgcca tattcgattt
     2221 gaattgctgc tgaataaagc gctgggactt ggcgcgcggt atttggcgac gggacattac
     2281 gcgcgcgtgc ggcagaccga tggccagtat caattgctgc gcggaatcga tccgaagaag
     2341 gatcaatcgt acgtgctgag tgtattgggt cagcctgaac tctcacacgc gctgtggccg
     2401 ctgggtgaaa tgaccaagcc gc
//
LOCUS       DASRTJ010000095        10037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_191490,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000095 DASRTJ010000000
VERSION     DASRTJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191490"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFF70_01840"
     CDS             complement(<1..407)
                     /locus_tag="VFF70_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048061038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HZY43463.1"
                     /translation="MSQPIHFFDEPEPAEERIGVYVCHCGTNISATVNVEEVRDWAGQ
                     RLKDRGVVISRDNKFMCSSLGQELIEKDIKELGLTRVVVAACSPHLHEQTFRGAAKRA
                     GLNPYLTELVSIREQVSWVHTDKGAATDKAKAVV"
     gene            complement(404..1399)
                     /locus_tag="VFF70_01845"
     CDS             complement(404..1399)
                     /locus_tag="VFF70_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HZY43464.1"
                     /translation="MNDKYLYYPGCSMESSAKAYSESLAAICEPIGLHLEEINDWNCC
                     GATEYIGISLTPAYSLITRNLALAAQQANGTRTVVAPCSACYLNLAKADYYMQERPIL
                     GENVNTALAAGGLHYDPGSLFIRHLLDVIINDVGLDVVKSKVVRPLTGLRVAPYLGCM
                     VPRPDYEHRWSNPEYPTELDQLLKTLGAEVIDFPLKTHCCGGHMTQIGPNTAFELIRR
                     LVSSADQYKADVMVTLCPMCQMNIDAYQGEMNRHFHTDYRMPIIFFTQLMGLAFGFDA
                     KQLGFGRELVGTREMLARIGVEAPEPIEAAPTRKPRKKEGLPMPRMPGDSEEVKP"
     gene            complement(1396..1998)
                     /locus_tag="VFF70_01850"
     CDS             complement(1396..1998)
                     /locus_tag="VFF70_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY43465.1"
                     /translation="MTEQTSPPKISPQHDPSNPNLRFLEEVSAHTAGVSRLEMCIQCG
                     TCGGSCPPAADMDHTPRMLFAMIRAGFREEALSSNTPWICVSCYHCVVRCPQGVHIAD
                     VMYTLKSMAIRAKLYQDSTAPDFSRSFVDNIEKYGRSYEFGLATRHYLKHFPLRLPGM
                     VPMGFGMLTKQRMDITPHKIDNIDQLHAILERAKEVEGLS"
     gene            2561..2953
                     /locus_tag="VFF70_01855"
     CDS             2561..2953
                     /locus_tag="VFF70_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZY43466.1"
                     /translation="MMTEAILGTALRYAIAARAARVTDLYFVVGQFSDVTDESVQFYW
                     DQLSPGTICEGARLHFDHAPAQLQCLICNHSFTLLNDRIPCPMCASEQVRMLSGDEFR
                     LDSINVATAAEVSPAAIGEVITVPEHEH"
     gene            3370..3756
                     /locus_tag="VFF70_01860"
     CDS             3370..3756
                     /locus_tag="VFF70_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZY43467.1"
                     /translation="METLSIDLPAMYADHHVVEVRSLLLNLPGVEDVYASSAFRIVRV
                     RVDPAQINEAVIRTKLNDAGYLGELSIQTEEWKSGSSQATVGNPFRHTAAYENIKSIA
                     FTQQSEETSRALWPCPGMGLIKKEEE"
     gene            3760..5772
                     /gene="cooS"
                     /locus_tag="VFF70_01865"
     CDS             3760..5772
                     /gene="cooS"
                     /locus_tag="VFF70_01865"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HZY43468.1"
                     /translation="MAKDKKTPQQFSIDPAAQQMLIRADSLGIGTAFTRAEAMPACNI
                     GAQGMCCKMCGMGPCRLTKDGSTGVCGATIDTIQARNFVRMVAAGAAAHSDHGRDMAF
                     LLKAVANNEAEGYSIRDVAKLRTAAAKSGIPIEKRSPNEIANDLADIYIAQFGQQRGE
                     LIPIKRAPVKRQKLWHERGVVPRGIDREVVEALHRTHIGDDQDPEHLLQHAIKTSMAD
                     GWGGSMIATDVADILFGTPAPVVGQANLGVLKADMVNVIVHGHEPILSEMIVAASQTP
                     EIIEYAKAAGAQGVNLAGICCTANEILMRQGIPAAGNFLHQELAILTGAVEAMVVDVQ
                     CIMQALVGLAENFHTKVITTSPKVKIKGATHMEFDEHKALTIAKDILKVAIDNFKNRG
                     AIEIPHDIEDLVPGFSHEYINYMLGGSYRSSFRPLNDAIMSGRIRGVAAIVGCNNPRS
                     QQDYLHTRVTRELLKQDVLVVETGCGAIASAKLGLLLGEAGLDQVGSGLREICETVGI
                     PPVLHMGSCVDNTRILTVLTQMVEEGGLGDDIDQIPAVGLAPEWMSEKALAIGTYCVA
                     SGAYVIFGGSSPLEGMPDKVTGSDDMLHYISAGWEKLYGGKMEFIADPDEMVRKTLEH
                     IDKKRAALGLPEWKADKFGRSGDKRMNEFEVLPIEEKLAAVYGSLN"
     gene            5898..8180
                     /gene="acsB"
                     /locus_tag="VFF70_01870"
     CDS             5898..8180
                     /gene="acsB"
                     /locus_tag="VFF70_01870"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HZY43469.1"
                     /translation="MSKYIATRAIRGANALVNETEAMLQKALAEKGSDCKVGFPNTAY
                     YLPTIYGMTGTPVETLGQLKPILQHARSLLHPLPSNKHWTPYLGETLDSGMATLLAAE
                     TLEAIRFVYGLQPEPMAGYSGSASFTSPDAVKDDGKAGGHLNGPIDDIQLRTWGIQLV
                     DGRMPGFAAIVGCAKSNEVAVKIVRELQRRNILTFLSGNVNGRSIIHQLMEEGVELGY
                     DTYTVPFGTDTLSAIYALGFAARSALTFGGMKGGMAREILNYNKDRVFAFVLALGEVD
                     DLKYAAAAGAINFGFPVIADTVIPEILPTGVTTYEHVVSMPFNEIGGKDDLERAEQLV
                     QKCIEVRGVKVKMSNVPVPVPYGSAFEGEVVRKNDMRIEFGGKYSRCFEYLHMTDLDA
                     ITDGKIEVVGPDFSTIEAQGHMDMGIVIEVAGRKMQQDFEPVLERQIHYFVNGASGIQ
                     HIGQRDIAWIRISTNAADKGFNLEHFGKILHARFHADFGAIVDKVQVTIFTEPTKHAE
                     WLVKARESYDFRNKRLADLTDDKVDEFYSCTLCQSFAPNHVCVVSPERLGLCGAYNWL
                     DCKASFSINPTGPNKPIKLGSSIDGRKGYWSGVNDYAKIGSHDVVQEVAMYSIMENPM
                     TACGCFESIVMLIPEANGVMVLSREDTSMTPAGMTFSTLAGVAGGGQQTPGVMGVGKY
                     YLISPKFISSDGGFKRVVWMSSVLKETMAEELKAVAIREGDPDLIEKIADERNVTSVE
                     DLLAWLEAHNHPAMAMEPMF"
     gene            8197..8817
                     /locus_tag="VFF70_01875"
     CDS             8197..8817
                     /locus_tag="VFF70_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZY43470.1"
                     /translation="MPKIDLRKQLKHLYNPPKNKFTVVDVPSMNFLMIDGEGDPNTSK
                     DFQEAMEALYSMSYTLKFALKFGKYGGKKYDYPVMALEGLWWTDDMTQFISGGKDKWK
                     WTVMIMQPDFITRSMFNAARKEVKEKRNPVALSKVRCAKYTEGPSAQIMYIGKFADEG
                     PTIQRLHDFIKESGHQLRSKHHEIYMSDPRRTAPEKLKTAIRQPFK"
     gene            8876..9388
                     /locus_tag="VFF70_01880"
     CDS             8876..9388
                     /locus_tag="VFF70_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZY43471.1"
                     /translation="MENTSERKLVRDLMTVGVETCAPDTPIIDLARAMLERNFEEIVV
                     LEEGNAAGVVAQDDIIYAFTREDFRSLKAEDVMRENVPQIPADIPISVAAQMMHDQGV
                     RVFWLMHHAGGIEYPAASISYKHLLRYLTANSDEELKDLGLAAARKAPLEQFIERRDA
                     AKQAANSRRK"
     gene            9470..>10037
                     /locus_tag="VFF70_01885"
     CDS             9470..>10037
                     /locus_tag="VFF70_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015556.4"
                     /note="part of a complex that catalyzes the cleavage of
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HZY43472.1"
                     /translation="MPIEIPKDKWPGSVREVTIGATAQDGGTRSHSITIGGEKALPFM
                     HFEAEMPHSPAVAIEIKDRKPDDWSPLLIDTWGEAINDPIKWAQAAEQAGADLIELTL
                     SLTAADGSPNTPQAAVKAVKDVLGATGLPLLVFGPGQAEIDNALLVPIAEATKGERLV
                     LGICEDKNYRTIVATAMANGHLVSSRTPM"
BASE COUNT         2384 a         2648 c         2832 g         2173 t
ORIGIN      
        1 accacggctt tggctttatc ggtggctgcg cccttgtcgg tgtgcaccca cgagacttgc
       61 tcgcggatcg acaccagttc cgtcaggtaa ggatttaacc cggcgcgctt tgccgcaccg
      121 cggaaggtct gctcgtgtaa atgcggcgag caggctgcga cgacgacccg cgtgaggcct
      181 agttccttga tgtccttctc gatcagttcc tggccaaggc tggagcacat gaacttatta
      241 tcgcgcgaga taaccacccc gcgatctttc aagcgctgcc cggcccaatc gcgcacttct
      301 tccacattga ccgtcgccga gatattagta ccgcaatggc acacgtacac cccaatacgt
      361 tcctcagcag gctcaggctc atcgaaaaag tgaatgggct ggctcatggc ttcacctcct
      421 cgctatcacc gggcatgcgt ggcataggca acccttcctt cttgcgcggc ttgcgagtgg
      481 gcgcggcctc gatcggttcc ggcgcttcaa cgccgatgcg tgccagcatc tcgcgcgtgc
      541 cgaccagttc gcggccaaag cccagctgct tcgcatcgaa accaaaggcc agccccatca
      601 attgggtgaa gaaaatgatc ggcatccgat aatcagtgtg gaagtgccgg ttcatttcgc
      661 cttgatacgc atcgatattc atctggcaca tcgggcagag cgtcaccatg acatcggcct
      721 tgtattgatc cgctgagctg actaatcggc ggatcaattc gaacgctgtg ttcggtccga
      781 tctgcgtcat gtgcccgccg cagcagtgcg tcttcaatgg aaagtcgatc acttccgcac
      841 cgagagtctt caacaactga tcgagctcgg tgggatattc aggatttgac cagcgatgtt
      901 cataatcggg gcgcggcacc atgcagccca aatagggcgc cacgcgcaag ccggtcagag
      961 gacgaaccac tttgctcttc acgacatcga gcccgacgtc attgatgatc acgtcgagca
     1021 agtgacggat gaacaacgag ccgggatcgt agtgcagacc gccagccgcc aacgcagtat
     1081 tcacgttctc acccaagatc ggtcgctcct gcatgtagta gtcggctttg gcgagattca
     1141 aataacaggc gctgcacggt gccactaccg tccgtgtgcc gttggcttgc tgggctgcca
     1201 aagcaagatt gcgcgtaatc agcgaatagg ccggtgtcaa actgatgccg atgtattcgg
     1261 ttgcgccgca gcaattccaa tcgttgatct cttcgaggtg taggccgatc ggttcacaga
     1321 tggccgccag tgattcgcta taagctttgg cgctgctctc catcgaacaa ccgggatagt
     1381 agagatactt atcgttcatg ataatccctc cacttccttc gcgcgttcca gaatggcatg
     1441 caattgatcg atattgtcga tcttgtgagg cgtgatgtcc atgcgctgct tcgtcaacat
     1501 gccgaagccc atcggtacca tgccgggcag gcgcagcgga aagtgcttca agtaatggcg
     1561 cgtagcaaga ccaaactcat aactgcgccc gtatttctcg atgttgtcga cgaacgaccg
     1621 agagaaatcg ggcgcggtgg aatcttgata gagtttggcc cggatggcca tgctcttgag
     1681 cgtatacatc acatcggcga tgtgcacgcc ctgcggacag cgcacgacgc agtgatagca
     1741 tgatacgcag atccacggcg tgttgctgct gagcgcttct tcccggaaac ccgcgcggat
     1801 catggcgaac aacatgcgcg gcgtgtgatc catgtccgcc gctggcggac aggatccgcc
     1861 gcacgtgccg cattggatgc acatctctaa ccgcgagaca ccggcggtgt gggcgctgac
     1921 ttcttccaag aaacgcaagt tgggattgga gggatcgtgt tgagggctaa tctttggggg
     1981 tgaagtctgt tcggtcaagg tgacctcctt gaatactggc aaggtgcaaa caaaaaacgc
     2041 aagcgatcct catgctcgat cgaggcctct tgcgttaaca ctcatgataa gtgttgggtt
     2101 taagcccgtc attcattgac ctcccaacta actcaagggt tagttgtggc gcagtgggca
     2161 tcccccgcat atcaaaaaaa caaatggttc aagtaaaatg atctggatca ttcggctcat
     2221 cggcttcaag ttatttacgc cgatgatagt ctcacaaagc caaccgcacg ataagtgatg
     2281 aatgtcacaa gctagtggga tgtgcctcct aacttccatc atgatcatca atacatatta
     2341 atgatacaca acaccaaatt tctattgacc tattaccgtg gaaatcgtca gcgtgtgatc
     2401 tcatttgcga ctcactcgat cgaactactc agtgacattt agtgcatgcg aagccgatga
     2461 aaaccacttc tcgtgtcata gcggctggca gtaatattag attggttatg tgatagcgaa
     2521 atgatcgtca agtgctttag cgatatatat gcatgaactt atgatgactg aagcaatcct
     2581 gggtactgcg cttcgatatg cgatcgcggc gcgcgctgcg cgtgttaccg atctctattt
     2641 tgtcgtcggg cagttttcag atgtgaccga cgagtccgtg cagttttatt gggatcagct
     2701 cagccctggc acaatttgcg aaggcgctcg attgcatttc gatcatgcgc ccgcccaatt
     2761 gcaatgcctc atctgcaacc actcgtttac actgctcaac gatcgaatcc cttgcccgat
     2821 gtgtgcgagt gagcaggtgc gtatgctcag cggtgatgaa tttcgtttgg atagcatcaa
     2881 cgttgctacg gccgctgaag tttcgccagc agcgatcggc gaagtgatca ctgtcccaga
     2941 acatgagcat tgacaaatgg ctttgcagat tttactctag cccacttgtt aaacacacaa
     3001 cacccaatgg cttcccgtcg ggtgttgtgc tttctggata acagattggt ttatttgtag
     3061 cgggtaacgt aataaacaga tcgagatgaa tctaatgtct cgtaataaat tgatcgaacg
     3121 gtatcgatgg gcaactctct gcctccgttg ctgctcgatc ggtcccgcca ttcccagacc
     3181 agcatgcatc gaagtaaatt tcgctcatct ctatcatcga aaataataag aacactgact
     3241 tcgatcaaaa aaaaggggac atctgtcact cggatagaca cttggatgtc attatactga
     3301 gtgcgtaaat tggcacaaat gcaaatgcat aacaattcga ttttcaacag agagggagcg
     3361 aggagccaga tggaaacttt aagtatcgat ctacccgcca tgtacgccga tcatcacgtc
     3421 gtcgaagtgc gcagtctctt gttgaacttg cccggcgtcg aagatgtata tgccagcagc
     3481 gcgtttcgca tcgtgcgcgt gcgcgtcgat ccggcgcaga tcaatgaggc tgtcattcga
     3541 acgaaattga atgacgccgg ttatctgggc gagctatcca ttcaaactga agaatggaaa
     3601 tccggatcgt cccaagctac cgtgggcaat ccattccggc atactgctgc ttacgaaaac
     3661 atcaagtcga ttgcttttac acagcaatcg gaagagacca gccgggcctt gtggccgtgt
     3721 ccgggcatgg gtttgatcaa gaaggaggag gaataaacca tggcaaaaga caagaaaact
     3781 ccacagcagt tctcaattga tcctgccgcg cagcagatgt tgatccgggc agattcatta
     3841 ggtattggca cggcattcac tcgcgctgaa gcaatgccag cctgcaatat cggtgcgcag
     3901 ggcatgtgct gcaagatgtg cggcatgggg ccgtgccgtt tgaccaaaga cggatcgacg
     3961 ggcgtgtgcg gcgcgacgat cgataccatt caagcgcgca acttcgtgcg catggtcgcc
     4021 gctggcgccg ccgcgcattc tgatcatggc cgcgacatgg ctttcttatt gaaggccgtg
     4081 gcgaataacg aagcggaagg ctattcgatc cgcgacgtgg cgaaactccg cacggcggcc
     4141 gcgaagtcgg gtatcccgat cgagaaacgc agcccgaacg aaattgccaa cgatctggcc
     4201 gatatctata tcgcccagtt cggtcagcag cgcggcgagc tgatcccgat caagcgtgct
     4261 ccggtgaaac gacagaagtt gtggcacgag cgcggcgtcg tgccacgtgg tatcgatcga
     4321 gaagtggttg aggcgctgca tcgcacgcat atcggcgatg atcaagaccc cgagcacttg
     4381 ttgcagcatg ctatcaagac gtcgatggcc gacggctggg gcggatcgat gatcgcgact
     4441 gatgtcgccg atattctgtt tggcacacct gcgccggtcg tgggtcaagc gaacctgggc
     4501 gtgttgaaag ccgatatggt caacgtgatc gttcacggtc acgaaccgat cttgagcgag
     4561 atgatcgtcg cggcttcaca gacgccggag attatcgaat acgctaaagc cgccggagcg
     4621 cagggggtca atctcgccgg catctgctgc accgcgaatg aaatcctgat gcggcagggc
     4681 attccagcgg cgggcaactt cctgcatcaa gaactcgcca tcctcacggg cgcggtcgag
     4741 gcgatggtgg tcgatgtgca gtgcatcatg caggcgctgg tcggcctcgc ggaaaacttc
     4801 catacgaaag ttatcaccac ttcgcccaag gtgaagatca aaggcgcgac gcacatggag
     4861 tttgacgagc ataaggcgct gacgattgcc aaagatattt tgaaggtcgc catcgataac
     4921 ttcaaaaacc gcggcgcgat cgagattcca cacgacattg aggatctcgt gcccggtttc
     4981 tcgcacgagt atatcaacta catgctcggc ggcagttacc gttcgtcttt ccgcccgtta
     5041 aatgacgcga tcatgagcgg ccggattcgc ggtgttgcgg cgatcgtcgg ctgcaacaat
     5101 ccgcgcagtc agcaagatta tttacacacc cgcgtcacgc gtgagttatt gaaacaagac
     5161 gtgctcgttg tcgagacggg ctgcggcgcg atcgcatccg cgaaacttgg cttgctctta
     5221 ggcgaagccg gactcgatca agttggctcc ggtctgcgtg aaatatgtga gaccgttggg
     5281 attccgccgg tgctgcacat gggctcgtgc gtggataata cccgcattct caccgtgttg
     5341 actcaaatgg tagaagaagg cggcctcggc gacgacatcg atcagattcc ggcggttggt
     5401 cttgcgcccg aatggatgag cgaaaaagca ttagcgatcg gtacatattg tgtcgcgtcg
     5461 ggcgcgtacg ttatcttcgg cggatcatct ccgctcgaag gcatgcccga taaagtgact
     5521 ggcagcgacg acatgctgca ttacatcagc gccggctggg agaaattgta cggcggcaag
     5581 atggagttca tcgccgatcc cgatgagatg gtgcgcaaga cgctggagca cattgataag
     5641 aaacgcgcgg cgctcggcct gcccgaatgg aaggccgaca agttcggtcg atcgggtgat
     5701 aagcgcatga acgaatttga agtgctgccg atcgaggaga agctggccgc agtttacgga
     5761 tcgttgaatt agaactggag aatgattaac cacagaggca cagaggcaca gagacacgga
     5821 gaaattttaa gttaaatact ctgtgcctca gtgtctcagt ggttcgcaat ctctaatatg
     5881 caacacggag agaaactatg tcaaaatata tcgccacccg agccattcgc ggcgcgaacg
     5941 ccctcgtcaa tgaaaccgaa gcgatgctgc aaaaggctct agccgagaaa gggtcggact
     6001 gcaaagtcgg tttcccgaat acggcttact atctgccgac gatctacggc atgaccggca
     6061 cgccggtcga aaccctgggg cagctcaagc cgatcttgca acacgctcga tcgttattgc
     6121 atccgctgcc atcgaataaa cattggacgc cgtatctggg cgagacgctc gacagcggca
     6181 tggcgacctt gctcgcggct gaaacgctcg aagcgatccg ctttgtgtat ggcctgcaac
     6241 ctgagccaat ggctggatat tccggcagtg catccttcac cagtcccgac gcagtcaaag
     6301 atgatggtaa agctggtggg catctcaacg gcccgataga tgatatccaa ctccgtacat
     6361 ggggcattca actggtcgat ggccgcatgc ccggcttcgc ggcgatcgtc ggctgcgcga
     6421 agtcgaacga agtcgcggtg aagatcgttc gagagttgca gcgccgcaac atcttgacat
     6481 tcctatcggg caacgtcaac ggtcgcagca tcattcatca attgatggaa gaaggcgtcg
     6541 aactgggcta cgacacatac acggttccgt tcggcaccga tacgttgagc gcgatctacg
     6601 cgctgggctt cgcggcccga tcggcgctca cgtttggcgg catgaagggc ggcatggcgc
     6661 gcgagattct caactacaac aaggatcgcg tgttcgcgtt tgtgttggcg ctgggcgaag
     6721 tggacgattt gaaatatgcg gcagcggcgg gtgcgatcaa cttcggcttc cccgtgatcg
     6781 cggacacggt cattccggaa attttaccga cgggcgtcac cacatacgaa cacgttgtct
     6841 cgatgccatt caatgagatt ggtggcaaag acgatcttga acgtgccgag cagttggtgc
     6901 agaagtgcat cgaagtgcgc ggcgtgaaag tgaaaatgtc caacgtgccc gtgcccgtgc
     6961 cgtacggctc cgcgttcgaa ggcgaggtcg tgcgcaagaa tgacatgcgc atcgaatttg
     7021 gcggcaagta ttcacgctgc tttgaatatc tgcacatgac cgatctcgat gcgatcaccg
     7081 acggcaagat cgaggtcgtt ggcccggact ttagcaccat cgaagcgcaa ggtcatatgg
     7141 acatgggcat cgtcatcgaa gtggccggcc gcaaaatgca gcaagacttc gagccggtgc
     7201 ttgagcgaca gattcactac ttcgtcaacg gtgcctcggg cattcaacac atcggccaac
     7261 gtgacattgc ctggattcgc atcagtacca acgcagccga caagggtttc aacctcgaac
     7321 acttcggcaa gatattgcac gcgcgcttcc atgccgactt cggcgcgatc gtcgacaagg
     7381 tgcaagtcac gatctttacc gaaccgacga aacatgctga gtggttagtc aaagcgcgtg
     7441 aatcatacga cttccgtaat aaacgcttag ctgatctgac tgacgacaag gttgatgaat
     7501 tctattcatg tacgctgtgc caatcgttcg ctccgaatca cgtgtgtgtt gtcagcccgg
     7561 agcgattggg gttgtgcggc gcatacaact ggctcgattg caaagcctcg ttcagcatta
     7621 acccgaccgg ccccaacaaa ccgatcaagc tgggcagctc gatcgatggg cggaaaggct
     7681 actggtcggg ggtcaacgat tacgccaaga tcggatcgca cgacgttgtt caagaagtgg
     7741 cgatgtattc gatcatggaa aacccgatga ccgcctgcgg ttgtttcgaa tcgatcgtga
     7801 tgttgatccc cgaagcgaac ggcgtgatgg ttctcagccg agaggacacc agcatgacgc
     7861 cggccggcat gacgttcagc acattggccg gcgtggcggg cggtggtcaa caaacgccgg
     7921 gcgtgatggg cgtcggcaag tattatttga tcagccccaa gttcatctcg tcggatggcg
     7981 gtttcaagcg cgtggtgtgg atgagcagcg tcttgaaaga gacgatggct gaggaattaa
     8041 aggccgtcgc gatccgcgaa ggcgatccgg atttgatcga gaagattgcc gatgaacgga
     8101 atgtaaccag tgtcgaagac ttactcgcgt ggcttgaagc acacaatcac ccggctatgg
     8161 cgatggagcc gatgttctga ttgcggagta atcaatatgc ccaagatcga tctccgtaaa
     8221 caactgaagc atctctacaa cccgccgaaa aataaattca cggtcgtcga tgtgccgtcg
     8281 atgaatttcc taatgatcga tggtgaaggc gatccgaata cttccaaaga ttttcaagag
     8341 gcgatggaag ccttgtactc gatgtcgtac acgttgaagt tcgcgttgaa gttcggcaag
     8401 tatggcggca agaagtatga ctatcccgtg atggcgctcg aaggtctgtg gtggaccgat
     8461 gacatgacgc agttcatcag tggcggtaaa gataagtgga agtggacggt gatgatcatg
     8521 cagccggact tcatcactcg atcgatgttc aacgcggcgc gcaaagaagt gaaagagaaa
     8581 cgcaatccgg tcgcgttgtc caaagtccgc tgcgctaaat acacagaagg acccagtgcg
     8641 cagatcatgt acatcggcaa attcgccgat gaaggcccga cgatccagcg gctgcacgat
     8701 tttatcaaag agagcggaca tcaactgcgc agcaagcatc atgaaatcta catgagcgat
     8761 ccgcgccgca ccgcgccgga gaaattaaag accgccatcc ggcagccgtt taaataaacc
     8821 tcaccccatc tcctctccct taaagggaga gggctagggt gagggtcgaa acaaaatgga
     8881 aaacacttct gaacgcaaac tcgtccgtga tctgatgacc gtcggcgtcg aaacctgcgc
     8941 gcctgatacg ccgatcatcg accttgcgcg cgccatgctg gaacgaaact ttgaagagat
     9001 cgttgtgctc gaagagggaa atgccgcggg cgtagttgct caagatgaca tcatctacgc
     9061 tttcacgcgc gaagactttc gatcgctcaa agctgaagat gtgatgcgcg aaaacgtgcc
     9121 gcaaattccc gccgacattc cgatctcagt cgccgcgcag atgatgcacg atcaaggcgt
     9181 gcgtgtcttc tggttgatgc accacgcggg cggcatcgaa tatccggcgg cttcgatttc
     9241 atacaagcat ttgctgcgct acttaacggc gaacagcgat gaagagttga aagatttggg
     9301 gttggccgcc gcacgcaaag cgccgctcga acaatttatt gaacgacgtg atgcggcgaa
     9361 acaggccgcg aatagtcgga gaaagtaaca tttaccgcag agcgcgcaaa gattttcttt
     9421 aggttttttt gcggtcttag cggcttggcg gttcaatcag gagtctctta tgcccatcga
     9481 aattcccaaa gacaaatggc ccggctcggt tcgtgaagta accatcggcg caacggccca
     9541 ggacggcggc acgcgatcgc acagcatcac gatcggtggt gagaaggcgc tgcccttcat
     9601 gcacttcgag gccgagatgc cgcattcgcc tgccgtcgcg atcgagatca aagatcgcaa
     9661 gccggatgat tggtcgccgc tgttgatcga tacctggggc gaagcgatca atgatccgat
     9721 caagtgggcg caggccgccg aacaagccgg tgcggatttg atcgaactga ctttaagttt
     9781 gacggcagcc gatggttctc ccaacactcc acaggccgcg gtcaaagcgg tgaaagatgt
     9841 attaggcgca accggtctac cgctgttggt ctttgggccg gggcaagccg agatcgataa
     9901 tgcgctgctc gtgccgatcg ctgaagcgac taaaggcgag cgcttggtgc tgggcatttg
     9961 cgaagacaaa aattatcgca cgatcgtggc gacggcgatg gcgaacggcc atctggtcag
    10021 ttcgcgcacg ccgatgg
//
LOCUS       DASRTJ010000096        12445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_342969,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000096 DASRTJ010000000
VERSION     DASRTJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12445
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342969"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFF70_01890"
     CDS             complement(<1..1168)
                     /locus_tag="VFF70_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43473.1"
                     /translation="MAHPSFPLPNYLQAQEEMFRAATRQLQSRSNPQSLSGAAEWLPD
                     NFYLVQQTCRQIREDMPASFYRQLPKRDAGPLQGYPRVYAVAQKLIVMRKARLDLDHI
                     TRFVQLYQDLAPLTTGELWALPVMLRLGLVEFLAQSVSRITDLPRANSLPTLTLPHAI
                     TGDETVANCITSLRMLATQDWQDFFESVSRVEHILRDEPANVYGIMDPETRDRYRKVI
                     EKLAHATGQDEQSVARQAIDLARTHSQPATPRAAHVGYYLLDAGRAQLDARVGYRVPL
                     ISRVRRGALDHPTPVYLGGIGLITFIGVLSASLYAREAGANLWQELAVGLLLLIPALT
                     VSVSLINWIITLAIPPRVLPKMDFEDNIPAEYKTLVVISSLLSSAAEVKSLLKQV"
     gene            complement(1172..2128)
                     /locus_tag="VFF70_01895"
     CDS             complement(1172..2128)
                     /locus_tag="VFF70_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZY43474.1"
                     /translation="MFNRILLPLDRSALAECVLPHTIAIARAFESQATLLHVMDTPRE
                     ARWRRAMDPLNWQIRKAEAKTYLHEVDLRLQAAGLLTETHILEGVAAEQVIGFSDTHA
                     SQLIILSSHGQSGLSDWGVSSVVQKIVLRACTSVMIVRAAQPRAPDVTGLRYQRILVP
                     MDGTQRAEYILPAAATLARAHDAQIVLAHIVQKPAMPRRTPPSREDVELADQLVERNQ
                     AEATQYLEQLRSRITGKVEARVLVSDHLAATLHELVEQEKIDLVLLSAHGVSSQTRGL
                     YGDVVSNLIAYGTTPLIIMQDSSQAQTAPTPIEVADGEPGRQ"
     gene            complement(2418..2810)
                     /locus_tag="VFF70_01900"
     CDS             complement(2418..2810)
                     /locus_tag="VFF70_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY43475.1"
                     /translation="MKGFVQNIEGITIKNEEFRRVLYTAKNCQLVVMALKPKEEIGME
                     VHKLDQFFRVEEGTGEAVLDGVRTVISAGFAVLVPAGTNHNIINTGNVPMKLYTLYAP
                     PNHRDGVVHHTRAEAETDNEHFDGKTTE"
     gene            complement(3012..4091)
                     /locus_tag="VFF70_01905"
     CDS             complement(3012..4091)
                     /locus_tag="VFF70_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZY43476.1"
                     /translation="MFNRILLPLDRSSLAECVLPHTIAVARAFESQVTLLNVMDVPRE
                     ARWRNAMDPLNWQIRKAEAKTYLHEVDLRLQAAGLLTETHILEGFAAEQIIGFSDTHT
                     PQLIILSSHGQSGLSGWNVSGVVLKVILRARTSIMIVRAYQLTPTEMTSLRYRRILVP
                     IDGSQRAECVLPMASTLARFHEAQILLAHVVRRPEMPRRTPPSREDVEVADRLVERNR
                     AEAIQYLNDLRSQLSGEVQARVLVSNHIAATLHELVEQEMIDLVVLSAHGHSGLTQWP
                     YGSVVISFITFGTTPLLIVQDLSQDEIQPSPAEIAAKEPWKAITHGPSVLSVAQLFAG
                     PRSSVSYSDAAVAVQIQAPIALQGG"
     gene            complement(4164..5657)
                     /locus_tag="VFF70_01910"
     CDS             complement(4164..5657)
                     /locus_tag="VFF70_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZY43477.1"
                     /translation="MNTLNGNDKQHRAILQDIAQRAMLERGLLPDFSAEALTELGKIQ
                     APAAVRNDESVCDLTNLLWGSIDNDDSRDLDQLTVAEAMPGNKIKILVAIADVDSIVK
                     NGSAIDEHARHNTTSVYTAAMIFPMLPEKLSTDLTSLNVNEDRLAIVIEMVLGAEGSL
                     QASAIYRARVRNHAQLAYNSVAAWLDGGSTPEGITTVNGLAENLHLQDKAAQSMRNFR
                     HIHGALSLETIEAKPIFDGDQIRDLEVEYKNRATDIIEDCMIAANGVTARYLTSRKFP
                     SIRRVVRTPKRWDRIVELAGEHKFGLPAEPDSKALDEFLIKQKAADPLRFPDLSLAVI
                     KLLGAGEYMAELPGDTAPGHFGLAVKDYAHSTAPNRRYPDLITQRLLKAAIAGSAWPY
                     RKDELDALATHCTEEEDAANKVERQVGKSAAALLLESRIGEQFDAIVTGASDKGTWVR
                     LLNMPVEGKLILGFEGLDVGDRLRVQLISINVERGFIDFKKVGSAGR"
     gene            complement(5670..6734)
                     /locus_tag="VFF70_01915"
     CDS             complement(5670..6734)
                     /locus_tag="VFF70_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZY43478.1"
                     /translation="MTDLTRRNFLKLAGGTLCAAAALPFTNLADRYGFKPTPERNMFL
                     GQSLSPLAAPPASLGRVATSSVNIYAEANRQSSFVRKAYRDEILSLVDQIAGEAVTSS
                     NAIWYQTTDGFVHSAFVQPVENILNPVDLAQNNFWGEVTVPFSDSRRAPSSASGLFLR
                     VYYTCVFRVIEVVTGTDDQPWYRLQDGITWSPGPYVPAAHIRRIDPTELTPLSPGASK
                     RVEVNLQSQIITAYENDVPVLTSSVASGYGANRTPSGTHHVLFKSLASRMIGGQGSGY
                     YDLPGVPFPTYFTGRAAAIHGAYWHNDFGHPRSHGCLNVPAPVARWFWRWTEPSAPFP
                     YEARRYDTPRGIIGTEIKVA"
     gene            6800..6964
                     /locus_tag="VFF70_01920"
     CDS             6800..6964
                     /locus_tag="VFF70_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43479.1"
                     /translation="MFKLRRRSQTQHRSIKSSLEISCPIGPAGSIEFREIYSGGVPFG
                     KLAALGAAVF"
     gene            complement(7166..7384)
                     /locus_tag="VFF70_01925"
     CDS             complement(7166..7384)
                     /locus_tag="VFF70_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43480.1"
                     /translation="MNKDVKSKPVKATHFIRGVGMEAALLLVAFLLGFVPMWLKSRES
                     AGRLSEVERHLDLASVQTALASAAVDTQ"
     gene            complement(7428..7973)
                     /locus_tag="VFF70_01930"
     CDS             complement(7428..7973)
                     /locus_tag="VFF70_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZY43481.1"
                     /translation="MNQIETADNAEDNPALSKIIERNIRTIIRLRLKAADQRGLQDRL
                     ADVITSFSGRMIFAYLHIIWFIVWILLNTGSFGVRPFDPFPYGLLTMIVSLEAIFLST
                     FVLISQNRFSREAEHRADLAMHIGLLTEHELTRVLQMLDIIQDKLGIANDQDSELADL
                     EMETKPEDVLAEIDRLQRRSK"
     gene            complement(8133..8630)
                     /locus_tag="VFF70_01935"
     CDS             complement(8133..8630)
                     /locus_tag="VFF70_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43482.1"
                     /translation="MSENLPNNELAVDRREEIVRTQQPGFASTQQVTRDVAAESRLRV
                     TQITRIMWTVVGILEILLGLRFMLKLIAANPGSGFAGFIYGITGAFTAPFNTLLGAPA
                     SEGSILEVTTLIAMAVYALFFWVVARVIVIATERTSARTTSRSVSEQTPGGPGNERTT
                     RTISS"
     gene            complement(8689..8913)
                     /locus_tag="VFF70_01940"
     CDS             complement(8689..8913)
                     /locus_tag="VFF70_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZY43483.1"
                     /translation="MDATKDILQGKWHELKGKVKGQWGKLTDDDVARLSGKTEELAGV
                     LQQRYGYGKAQAESEINNWLSKHDSKPTKA"
     gene            complement(8994..9143)
                     /locus_tag="VFF70_01945"
     CDS             complement(8994..9143)
                     /locus_tag="VFF70_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43484.1"
                     /translation="MNITKNLGMLLLAIYLILLGLMGLASIAIPGIIMAILALAAGIL
                     ILIGR"
     gene            complement(9240..9500)
                     /locus_tag="VFF70_01950"
     CDS             complement(9240..9500)
                     /locus_tag="VFF70_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZY43485.1"
                     /translation="MGLLSWIVVGAIAGWLAGNLVKGTGFGCLGDIIVGVVGGLVGGF
                     LASQLFNMPDAVNGFNLGSIVVAFLGAVVVIIIVRLLRGGRS"
     gene            complement(9653..10942)
                     /locus_tag="VFF70_01955"
     CDS             complement(9653..10942)
                     /locus_tag="VFF70_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43486.1"
                     /translation="MKLYLLLLTLALTLSACIVTADPAPSGSVNNANSAAAQQARADA
                     AMQQARQMEADAQAAAANETAQAQAAQAVRALAATATAQALNVARAQLAMTADAANFP
                     STQSAVFAQQTAQAIQAKATVDARAMLALQEQAQTTATSQALVAQQTAEALQANAIVD
                     AGAMLAAQQQSQATATAQALQRNNLTANQVADTSQQQRELGAWLIPVLAVSAFGLVLL
                     LGAKFVSGLIDRANDGRRLENQRLAQIASLLAAPTETIVFVGDPQTAFGKMHLLNTPA
                     NSEVYDTGKPMASTAPLIDVEEPPMVVILSNGEVVLPDRAEAREEAARCKLAMKLLRD
                     AIDHEGGHSNHIPPAEQLGWPSRAWTIAIAILRPYGVEILHEQDRETYLVGQSTTLEA
                     LYIAVGERYLTCFPPSVENVIKGSTISQRDLSASTSG"
     gene            complement(10965..11699)
                     /locus_tag="VFF70_01960"
     CDS             complement(10965..11699)
                     /locus_tag="VFF70_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZY43487.1"
                     /translation="MSKAYEVMTHLVATCGLDAHACDVAATMRDLNIGDVLIVEDGKL
                     RGIVTDRDLTIQALTGKDDPQQTPIRKYMTDKVVTGEPAWSLEQVAHTMGKHQIRRLP
                     IVQAGQLVGIISLGDIARHNDTKYAAAKSLQAISTPIGSSALKPSSRGSVLPFLALAA
                     LVTGAFAWLTLNHSGQAVRKQIANSDLYHSAQDAVNATREKVDEAAQSKAVHELRDQM
                     HSKLNQLTAQVAALQAKPKRKHSWFS"
     gene            complement(11899..12045)
                     /locus_tag="VFF70_01965"
     CDS             complement(11899..12045)
                     /locus_tag="VFF70_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZY43488.1"
                     /translation="MLWTIVVILVVLWLLGLLGGVGGSLIHLLLVVAVIVIVVNLLQG
                     RRVS"
     gene            complement(12230..12388)
                     /locus_tag="VFF70_01970"
     CDS             complement(12230..12388)
                     /locus_tag="VFF70_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZY43489.1"
                     /translation="MFNTVFMFGLMMSVPAALPVWPYSRHWGFNPSVILGLLLIVLLI
                     LSAVDVNF"
BASE COUNT         2696 a         3475 c         3438 g         2836 t
ORIGIN      
        1 ccacttgttt gagcaacgac tttacttcgg ccgcactgga caacagcgag gagatgacca
       61 ccagtgtctt gtactcggcc gggatgttat cctcaaaatc catcttgggc aaaacgcgtg
      121 gtggaatggc cagcgtaatg atccaattaa tcagactgac agacacggtg agcgctggga
      181 tgagcaacag cagtccgacc gccagttctt gccaaagatt cgcgccggct tctctcgcat
      241 agagactggc gctgagcaca ccgatgaacg tgatcaaccc aatgccgccg agatacaccg
      301 gtgttggatg atccagtgcc ccacggcgca cgcgcgagat caggggcaca cgatagccca
      361 cgcgcgcgtc gagctgcgca cggcccgcat cgagcagata gtagccgacg tgcgccgcgc
      421 gcggtgtggc cggctgtgaa tgggttcgag ccaggtcaat cgcttggcgc gcgacggatt
      481 gctcatcctg gccggtggcg tgcgccaact tttcgatcac cttgcgatag cggtcgcgcg
      541 tctccggatc cattatgcca tagacgttcg ctggctcatc gcgcagaatg tgttcgaccc
      601 ggctgacgct ctcaaaaaag tcttgccaat cctgggtggc cagcatgcgc agactcgtaa
      661 tgcagttggc gacagtctca tcaccggtga tcgcgtgggg caaggtcagc gtgggcagcg
      721 aatttgctcg gggcaaatcg gtgatgcggc tgacgctttg cgccaggaac tcgacgagcc
      781 ccaggcggag catcacgggc agtgcccaca attcgcccgt tgtcagtggc gcgaggtcct
      841 gatagagctg cacaaagcgc gtgatgtgat ccagatcgag ccgcgccttg cgcatcacga
      901 tcaatttttg cgcgacggca tagacacgcg gataaccctg cagtggccct gcgtcgcgct
      961 tcggcaattg gcggtagaaa ctggccggca tatcctcgcg gatttggcgg caggtttgtt
     1021 gtaccagata gaaattatcc ggcagccact cggcggcccc ggagagcgat tgggggttag
     1081 acctggactg caactgccgc gtcgctgcac gaaacatctc ttcctgggcc tgcaaataat
     1141 tgggtaacgg aaaggacgga tgggccatgc tttattgtct tccgggttcg ccgtcagcca
     1201 cttcgattgg ggtgggcgct gtttgggctt gcgaactatc ttgcatgatg atcagtggcg
     1261 tcgtgccata cgcaatcaaa ttgctgacca catcgccata aagcccgcgg gtttgactgg
     1321 agacaccatg cgcgctgagc agcaccaggt caattttttc ttgctcgacc aattcatgca
     1381 acgtggcggc gaggtggtcg ctgacgagga cgcgcgcttc aactttcccc gtaatccgcg
     1441 atcgcagttg ctccagatac tgggtcgctt ctgcctgatt gcgctccacg agctggtcag
     1501 ccagttcgac atcctcgcgg ctgggtggcg tccttcgggg cattgcgggc ttctggacga
     1561 tgtgcgctaa cacgatttgc gcgtcgtgtg cacgcgccag ggtggcggcg gcgggcagga
     1621 tgtactcggc gcgttgggtg ccatccatcg gcacaaggat gcgctgatag cgcaagccag
     1681 ttacgtccgg cgccctgggc tgagccgcgc gcacgatcat caccgaagtg cacgcgcgca
     1741 gaacaatttt ctgtacgacg ctgctcacgc cccagtcact caagccgctt tgcccgtgac
     1801 tgctcaggat gatcagttgg gacgcgtggg tgtctgaaaa cccgatgact tgttcagccg
     1861 caaccccttc gaggatgtgc gtttcagtga gcaagccggc ggcttgcagg cgcaggtcca
     1921 cttcgtgcaa atacgttttc gcttcggctt tgcggatttg ccaattgagt ggatccatcg
     1981 cacgccgcca gcgcgcttcg cgaggcgtat ccatcacatg cagaagtgtg gcctgggatt
     2041 cgaatgcgcg cgcgatggcg atcgtatgtg gtaacacgca ctcggcgagc gccgagcgat
     2101 ccagggggag gagaattcgg ttaaacatcg ggcttgcctc atttactcat ggttcggtca
     2161 tttatccagc gggtttcgga aaaccgctga ccagttgggt ctagatttcc atcgaggatt
     2221 ccaataggga tagactcttc cttgaacatc cgagagcggc gtcacatgat actcgacctg
     2281 ctcaccgagc gtgacgacag acagataaat acggcgcact cacgccccgc gattgcgaag
     2341 cgcgtcctgg gccgtgcgcg cgtggttata ctcgcccgcc tcaacgcgga caatcacccc
     2401 agcgtccaat cagatctcta ttccgtcgtt ttaccgtcga agtgttcgtt gtccgtctcc
     2461 gcttcggcgc gggtatggtg gacaacgccg tcccgatgat tcggcggcgc atagagcgta
     2521 tagagcttca ttggaacatt gccggtattg atgatgttgt gattcgtccc ggcagggacg
     2581 agcacggcga agcccgcgct gatcaccgtg cgaaccccat cgagaacggc ctcgcccgtc
     2641 ccttcctcca cacgaaagaa ctgatcgagc ttgtgcactt ccatcccgat ttcttccttg
     2701 ggtttcaacg ccatgacaac gagctgacag ttcttagctg tgtaaagtac ccggcgaaat
     2761 tcttcattct tgatggtgat gccttcgatg ttttgtacga aacctttcat agcaactcct
     2821 ccagttgacg actttaacat cgtcgttagt tcgattgcct caaagaagag gcaccttttt
     2881 tatgacgccc aacactaact ttcagcggcc ctccgtcgcg tttcggcaat tcgcgataga
     2941 aactggccgg catatcctcg cggatttggc ggtaggtttg tcgtaccaga tagaaattat
     3001 ccggcagcca ctcagccgcc ttggagagcg attggggctt ggatttggac tgcaactgcc
     3061 gcatcgctgt acgaaacaga acttcttggg cctgcaaata attgggcaac ggaaaggacg
     3121 gatgggccat gcgtgattgc cttccatggt tccttggcag caatttcagc tggacttggc
     3181 tgaatctcgt cctgcgaaag gtcttgcaca atcaacaaag gggtggttcc aaacgtaatg
     3241 aaactgatga ccacactacc atagggccat tgggtcagac cggaatgacc atgagcgctc
     3301 aagactacca ggtctatcat ctcttgttca accaactcat gcaatgtggc ggcgatgtga
     3361 ttgctgacga gaacgcgcgc ttgaacttcc cctgatagct gtgagcgcag gtcattcaag
     3421 tactgaattg cctctgcacg attgcgctcc acaagccggt ctgccacttc gacatcctcg
     3481 cggctgggag gtgtccgccg aggcatttcc ggtcgcctga caacatgcgc cagcaagatt
     3541 tgagcctcgt ggaaacgggc gagggtgctc gccataggca ggacgcactc ggcacgctgc
     3601 gatccgtcaa tgggaaccag gattcgacga tagcgcaggc tggtcatctc cgtcggggtt
     3661 agctgataag cgcgcacgat catgatagaa gtgcgggcac gcaggatgac cttcagcacg
     3721 acaccgctca cgttccagcc actcaagccg ctttgcccgt gactgctcag gatgatcagt
     3781 tgaggcgtgt gggtgtccga aaacccgatg atttgttcag ccgcaaaccc ttcgaggatg
     3841 tgcgtttcag tgagcaagcc ggcggcttgc aggcgcagat ctacttcgtg caaatacgtt
     3901 ttcgcttcgg ctttgcggat ttgccaatta agcggatcca tcgcattccg ccagcgcgct
     3961 tcgcgaggca catccatcac atttaggagc gtgacttggg attcgaacgc gcgcgcgacg
     4021 gcgatcgtat gtggcaatac gcactcggcg agcgacgagc gatccaaggg gaggagaatt
     4081 cggttaaaca tcgggctttc ctcatttagc aacaaaaaac actgatcggt tgacctggcc
     4141 gatcaggcgc gcggaataat cggtcaacgt ccggctgaac cgactttctt gaaatcaata
     4201 aagccccgct caacattgat cgatatcaac tgcacgcgaa gccggtcgcc gacatccaga
     4261 ccctcgaagc caagaatcaa tttcccttca accggcatat tcagcagccg cacccacgtg
     4321 cctttatcag aagcacccgt gacaatggcg tcaaactgtt cgccgattct cgattcaagg
     4381 aggagcgccg ctgcagattt gccgacctgc cgttcaactt tgttggcggc atcctcttct
     4441 tcggtgcagt gtgtcgccag agcatccagt tcatccttcc gatagggcca cgcactaccc
     4501 gctatggcag ccttcaataa gcgttgcgtg atcagatcgg gataacgacg attcggggcg
     4561 gtagaatggg catagtcctt gacggcgaga ccaaagtggc ccggagcggt gtcaccgggc
     4621 agttcggcca tgtattcacc cgctcctaag agtttgatca ccgccaggga taagtctggg
     4681 aagcgcagcg gatcggcggc tttctgcttg atgagaaact catccagcgc ttttgaatcg
     4741 ggttcagcag gcagcccaaa cttgtgctcc ccggccagct cgacaatccg atcccagcgt
     4801 ttgggcgtgc ggaccacccg tcggatcgaa gggaattttc tggacgtgag ataccgggca
     4861 gttacgccgt tggccgcgat catgcaatcc tcgataatgt ccgtcgcgcg gttcttatat
     4921 tccacttcga gatcacggat ttgatcgccg tcaaagatcg gtttcgcctc aatggtctcc
     4981 aaactgagcg ccccgtggat atgccggaaa ttccgcatac tttgggccgc tttgtcctgc
     5041 aggtgcagat tctcagccag gccattgacg gtcgtgatcc cttccggcgt actgccaccg
     5101 tccagccagg ccgccacgct gttataggcc agctgcgcgt gattgcggac acgcgcccga
     5161 tagatagctg aagcctgaag agagccctcc gcccccagca ccatttcaat gacgatggcc
     5221 aggcgatcct cattgacgtt cagtgaggtc aaatcggtgg acagcttttc aggcagcatg
     5281 gggaatatca tggccgcagt atagaccgag gtggtattgt ggcgcgcgtg ctcgtcaatc
     5341 gctgatccgt ttttgacgat cgagtccacg tcagcgatag ccaccagaat cttaattttg
     5401 ttgccgggca tcgcttcggc tacggtgagc tgatcgaggt cgcgggagtc atcgttatcg
     5461 atggagcccc acagcagatt cgtcaggtca cacacggatt catcgttcct gactgcggcc
     5521 ggtgcctgaa tcttgcccag ctcggtaagt gcttcagccg aaaagtcagg gagtaagccg
     5581 cgctcaagca tcgctctttg agcgatgtcc tgtaaaatag cgcggtgctg tttatcattc
     5641 ccattgagcg tgttcatgtg ctgtccttat caagccactt tgatctcggt gccgattatg
     5701 ccgcgcggcg tgtcatagcg tctggcctca taggggaagg gcgcggatgg ctcagtccaa
     5761 cgccagaacc agcgagctac tggtgcaggc acattcaagc agccgtgact gcgcggatgg
     5821 ccgaagtcgt tatgccagta agcgccatgg atcgccgcag ctcgcccagt gaaataagtt
     5881 gggaacggca caccgggcag gtcgtaataa ccgctgcctt gcccgccgat catgcgtgag
     5941 gccagcgact tgaacaggac atggtgtgtg ccgcttggcg tgcggttcgc gccgtagccg
     6001 ctggcgacac tcgaagtcaa caccgggaca tcgttttcgt aggccgtgat gatctgagat
     6061 tgcaaattga cctcgacgcg tttgctggct cccggcgaaa gcggcgtcaa ttcagtagga
     6121 tcaatgcggc ggatatgcgc ggcgggcacg tacggaccgg gcgaccaagt gataccgtct
     6181 tgcagtcgat accacggttg atcgtctgtg cctgtcacaa cctcgatgac gcgaaaaacg
     6241 cacgtatagt acacgcgtag aaataggccg ctggctgaac tcggagctcg gcgtgaatcg
     6301 ctgaacggga ctgtcacttc accccaaaaa ttgttctgcg ccagatcgac cggattgagg
     6361 atattttcga ccggctgcac gaaggccgag tggacaaagc cgtcagtcgt ttgataccag
     6421 atggcattag acgaggtgac cgcttcgcca gcgatctgat caactaatga aaggatctca
     6481 tcccgataag ctttgcgcac gaaggatgat tgtcgattgg cctcggcata aatgttgaca
     6541 cttgaagtgg ccacccggcc cagcgaagca ggcggcgcgg ccaatggact caggctctgg
     6601 cccagaaaca tattcctctc gggcgttggt ttgaagccat accgatcggc cagattggtg
     6661 aagggtaagg ccgccgcggc acacagcgta ccgccggcca gtttaagaaa attgcgtcga
     6721 gtcagatcag tcatgcttgc ctctgtgtcc ttcattaata tatgcgctcc gtgctaagag
     6781 tagaggcata ggtaacagcg tgttcaaact tcgtcgtcga agtcaaactc agcatcgatc
     6841 gatcaaatct tcgctggaga tcagctgccc gatcgggcca gctggctcga tcgagttccg
     6901 ggagatttat tcaggcggtg tcccgttcgg caaattggct gcgcttgggg cggccgtgtt
     6961 ttgatcgata tcggatgggt tagtcccgct cagattggtg gcgtttgaat cgatcgggct
     7021 caggtcggtt gtgctcaccc cggaagatcg cggccatctt gagcgcgggg gattcaaaag
     7081 attttctaat tgcgagagag taaagtttgc cattttcact gccttcatga gccagcagtc
     7141 tcacaccggg ccattaagtt ttacgttatt gcgtgtcaac ggcggccgat gctaaggcag
     7201 tctgtacgct ggccaaatct aagtggcgct cgacttcaga caagcggccg gccgattcgc
     7261 gcgacttgag ccacatcgga acaaagccga gcaggaaggc gacgagcagc aaggctgcct
     7321 ccatgccgac acctcgaatg aaatgagtag ctttaaccgg tttcgatttc acatctttgt
     7381 tcatcgtaac tccttcaggt aaatgattac tgcgtgtgaa gccacactca ctttgaccgc
     7441 cgttgcagcc gatcgatttc agccagcaca tcttcgggct tggtttccat ttccaagtcg
     7501 gccaattcgc tgtcctgatc gttcgcgatg cctaatttgt cttgaatgat gtcgagcatt
     7561 tgcaacactc gcgtcaactc atgttcggtc agcaaaccga tgtgcatcgc caggtcggcg
     7621 cgatgctcgg cttctcggct aaaacgattt tggctgatca gcacaaaggt tgacaggaag
     7681 atcgcttcca gggacacaat catggtcaag agcccgtagg gaaacggatc gaaaggccgc
     7741 accccaaaac ttccagtgtt caataagatc catacaatga accagatgat atgcaaatac
     7801 gcaaagatca tgcgccctga aaaggaagtg atgacatccg cgaggcggtc ttgcaagcct
     7861 cgctggtcag cagccttcag gcgcagccgg ataatcgtgc gaatgttccg ctcgatgatc
     7921 ttgctcagag cgggattatc ttcggcattg tcggccgttt ctatttggtt cacacgactt
     7981 tgctgcatat gacggatgtc cttaacgctg atcactgagc atggcgtctg gcgcttcacg
     8041 caggaaaaga ttttggttat ggtccggtgg ttccaacaac cgagagcttg agcaattcgc
     8101 tgagtgctca aaccctcgac gatcgatcgt agttaactag agatagtacg tgtagtccgc
     8161 tcattgcccg ggccaccggg cgtctgctcg ctcacggatc gcgatgtcgt gcgggcactc
     8221 gtccgttcag tcgcgatgac aatcacgcgc gcgactaccc agaagaacag cgcataaacg
     8281 gccatcgcaa tcaaggtggt cacttctaag atcgatcctt cggaggcggg cgcaccgagc
     8341 aaggtgttaa atggcgcagt gaaggctccg gtaattccat agatgaaacc ggcgaagccg
     8401 ctgcccggat tagccgcaat caacttcaac atgaagcgca ggcccaacaa aatttcgagg
     8461 atccccacca cggtccacat gatccgggta atttgcgtca ctcgcaggcg actctccgcg
     8521 gcgacgtcgc gcgtgacctg ttgcgtggac gcaaaacccg gctgttgtgt tcttacgatt
     8581 tcttcgcggc gatcgaccgc gagttcattg ttcggtaaat tctctgacat ggtatctcct
     8641 taaggtgctt gctagaatat ccaaagtctt gccagagctg gctaatcctt aggctttggt
     8701 tggctttgag tcgtgcttgc tcagccagtt gttgatttct gattctgctt gcgcttttcc
     8761 gtaaccatag cgctgctgca acacgccggc gagttcttct gtttttccgc tgagtcgagc
     8821 cacatcatca tcggtgagtt ttccccactg gcccttcact ttgcctttga gttcatgcca
     8881 cttgccttgc aatatgtctt tggtcgcgtc catgttagat tctccttagt gtgtatgcgg
     8941 tttgatattg agatctgagt tggtgcgctc ggccgccaac agccgttcga cttttatcgg
     9001 ccaatcagga ttaagatacc ggcagccagc gcaagtatcg ccatgataat gccgggaatc
     9061 gcaatgctgg ccaggcccat gaggccgagc aggatcagat agatcgccag caagagcatg
     9121 cctagattct ttgtgatatt catgttgttt acctcctcag gtaatgacta cgatttgaac
     9181 tgcattaata aggtatgtcg cactgtccat tgactggcac ggttcgacca gcgttgagac
     9241 taacttcgac cgccacgcaa caggcgcaca atgatgatca ccacaaccgc tccgagaaat
     9301 gcgacgacga tgctgcccag attaaagcca ttgaccgcat ccggcatgtt aaagagctgt
     9361 gacgccagga agccgcccac cagtccgccg acgacgccca cgatgatgtc acccagacag
     9421 ccgaagcccg tgcctttcac caaatttccg gctaaccagc cagcgatagc cccaaccacg
     9481 atccacgata acaaccccat tttattttct ccttaacgta cgtaggttga gaaagatcca
     9541 tccggctagg tacgccggtc gctcgttaac aagttgttgt gaactcgata ttcgatcagg
     9601 ccagcccctt tcctaagaat tcattccgat ctgaatcccg atcggctgaa gctcacccgc
     9661 tggtgctcgc cgataaatcc cgctgggaaa ttgtcgagcc cttgataaca ttctcgaccg
     9721 atggcggaaa gcaggtcaag tatctttcgc ccacggcaat gtacaaggct tccaaagtgg
     9781 tggattgccc caccaagtat gtttcgcgat cttgctcgtg caaaatttct acaccatatg
     9841 gcctgaggat tgcgatagct atcgtccacg ccctgctggg ccaacccagc tgctcggctg
     9901 gcggaatgtg attcgagtgc ccgccctcat gatcgatcgc gtcacgcagc aatttcatgg
     9961 ccaacttgca acgcgctgct tcttcacgcg cctccgcgcg atcgggcaac accacctcgc
    10021 cgttggacag aataaccacc atcggcggtt cctcaacatc gattaaggga gcggtactcg
    10081 ccataggttt tcccgtgtcg taaacttcac tattcgccgg tgtgttcaat aaatgcattt
    10141 tgccgaaggc tgtttgtgga tcaccgacga agacaatggt ctcggtgggt gccgccagca
    10201 gtgaagcgat ctgggccagc cgctgattct ctaatctgcg tccgtcattg gcccgatcaa
    10261 tcagcccgct cacaaatttc gcccccaaca ataggaccag cccgaaggcg ctgacggcga
    10321 gcacgggtat caaccacgcg cccaactcgc gttgttgctg tgaggtgtcg gctacttgat
    10381 tcgcagtcag attgttacgt tgcagagcct gcgcggtggc ggtggcttga gattgttgct
    10441 gcgctgctaa catggcaccc gcatcaacaa tggcattcgc ctgtagtgcc tcggcagtct
    10501 gttgagcgac cagcgcctga gacgtagctg tcgtctgggc ttgctcctga agtgcgagca
    10561 ttgcccgcgc atcaacggtc gctttcgcct gtatcgcttg tgcggtctgt tgggcgaaca
    10621 cagccgactg tgtggaggga aagttcgcgg catcggccgt catggccagt tgcgcgcgcg
    10681 ctacgtttaa ggcttgggca gtggctgtgg cggcaagtgc ccgcacagcc tgggcagctt
    10741 gagcttgtgc ggtctcattg gctgcagcgg cttgcgcatc ggcctccatc tgtcgggctt
    10801 gttgcatcgc agcatcggcc ctggcttgct gtgctgctgc gctgttagca ttgttaacgg
    10861 atccagatgg agcaggatca gcggtgacga tgcaggccga tagagtcaag gccaaagtta
    10921 acagcagtaa gtaaagtttc atcgtcgtcg atcagtgttt tgatttacga gaaccacgag
    10981 tgtttgcgct tcggtttagc ctgcagcgcc gccacttgcg ccgtgagttg attgagcttg
    11041 gaatgcattt gatcacgcag ctcgtggacc gctttcgatt gtgcggcttc atcgactttc
    11101 tcgcgcgtgg cgttcactgc atcttgcgcc gagtgataca ggtcactgtt cgcgatttgc
    11161 ttgcggaccg cttggcctga atgattcaac gtcagccacg cgaaggcgcc tgtgaccagt
    11221 gcggcgagcg ccaaaaacgg taaaacgctg ccgcgacttg agggctttaa ggccgaagaa
    11281 ccgatcggcg ttgagatagc ctgaagtgat ttcgccgcgg catatttggt atcattgtga
    11341 cgggcgatgt cgccgagtga aataatgcca accaattgtc cggcttgaac gatcggcaaa
    11401 cggcggatct gatgcttgcc catcgtatga gccacttgct ccaaactcca ggccggttca
    11461 ccggtgacca ctttgtccgt catatacttg cggatcggcg tttgctgcgg atcatctttg
    11521 ccggtcagcg cttgaatggt caaatcgcgg tcagtcacga tgccgcgcaa cttgccatct
    11581 tccactataa gcacgtcgcc aatattgaga tcacgcatcg tggcggccac gtcacaggcg
    11641 tgcgcgtcca gaccacaggt tgcaactaag tgcgtcatta cttcataagc tttcgacatg
    11701 attcacctcc aaagatgttg tttctgaatt tgaaacttaa gcacgcggat cagtctgtcc
    11761 gctggctgcc cggtattacg gttgatgcag atttacacaa acctgctggc tgtgacgccc
    11821 acactcgcct gatgggttat cggcagagtg tgggctctcg caccggatcg attggcgggc
    11881 tgcctgccca tctgcggatt agctaacgcg gcggccttgc aataggttca cgacaatcac
    11941 aatcacggcc accacgagca ggagatggat taagctgcca cccacgccac ccaggagacc
    12001 taacaaccac agtaccacca gaatcaccac aattgtccac aacatttgaa cacgctcctc
    12061 tactaatttt ttggaaatca atatcgattg ctagcgacaa gtgagcgagc tagctctgaa
    12121 cgccaccacc gcgtcgatcg cgtgctcgtt ataaccgacc cactacaaac aggaccaaca
    12181 caatcacaac aatcaggtat aacatgtgga ctctcctttc ctgtttattt tagaaattga
    12241 catcaacagc cgacaaaatc aataaaacga taagcaataa gcctaaaatg acacttggat
    12301 tgaagcccca gtgtcgactg tacggccaga ccggcagagc ggccggaact gacatcatta
    12361 gcccaaacat gaaaacagtg ttaaacattt ttggctctac aattctccgg accgactgcg
    12421 tgacttagca cgagcgaatt ttaag
//
LOCUS       DASRTJ010000097         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_267291,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000097 DASRTJ010000000
VERSION     DASRTJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267291"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..2117)
                     /locus_tag="VFF70_01975"
     CDS             complement(246..2117)
                     /locus_tag="VFF70_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZY43490.1"
                     /translation="MPRKPLLLVSIMSAALIGATSIGLARPIDVAPANETGVASTLAP
                     SKIAPWVIDHTDRGAQAEFLVVLNDQADLSPAHQLSSKIDKGQFVYQALLNKAQTTQQ
                     SLLAYLAVQHIQYQSFYIVNAILVTGDRDLALKLADRSDVARIEGNPSIHNPLPRSAS
                     TPDQSTPSSISSIEPGVDYIHAPQVWALGFTGQGVVIGNQDTGMQWDHPALQPHFRGW
                     NGITTTFDYNWHDSIHSTSNGSSCGVDSPTPCDDYGHGTHTTGTAIGDDGAGNQIGVA
                     PGAKWIGCRNMDVGDGTPATYLECFQFFLAPYPVTETYTQGLPALAPDITTNSWSCPT
                     SEGCSFNTLQAAVEAQRAAGIMTIAAATNDGPGCSTISEPIALYDASYTVGALNTGSD
                     SIASFSSRGPVTIDGSQRIKPDLAAPGNPVRSSVPGNGYGSMSGTSMATPHVAGAIAL
                     LWSARPALKNQITATEQVLNNSAVHISTTDCSSGGVPNNVYGYGRLDIYTAIIGARIS
                     PEADHQANLAGQVVSYTLRFTNTDYISNSYVISITSGWPITITPATISNLGVNSGTAI
                     TAFVQLPVSITFNISDVASINVRLQSDAQRAVTTTLTSTAIAPPYIYYFPIIRRDSN"
     gene            2211..3236
                     /locus_tag="VFF70_01980"
     CDS             2211..3236
                     /locus_tag="VFF70_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY43491.1"
                     /translation="MHRERISDDIYVFTSGLYAQVTAGAIITSAGAVIIDTLPFPAET
                     RQVIDYVERRSPQGIRFVIYTHYHADHTYGGYLFKNAQVISHHLCRQLLIERGEAGME
                     LARAQNPQLADVKLRLPDIVFNGSLTLRLGNKTIELAQSPGHSPDMITAYVKEDRILF
                     ASDTVMPVPYIVDGDTNQMAASLTKLKSMSLENVVQGHGEIVLRGEISEAVNSSLNYL
                     QKMQGATANALLLNQSRDDLIREYDIESCGKARTSLNGLVQQLHAANLITTYDRLNDA
                     RTHGGTNHKLRALVRLAVTKPVRKVKGKRKGAAKKTTARSSEAALKSKTGKAANSRSV
                     TKPARYR"
     gene            3233..>3733
                     /locus_tag="VFF70_01985"
     CDS             3233..>3733
                     /locus_tag="VFF70_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43492.1"
                     /translation="MTSSRVDALLKKIDQLNADERRELLARLQADQLALPFVASAESF
                     QANTLIGQPDTIILFDGGSLGNPGKGYGSYALKNNHSKVSLQTRTKSKEPAQGWQITR
                     LNFGDNTTNNEAEYATLMQALDDLHSRIERADRLPQEFSIEIRGDSTLVINQLSGEWK
                     SKDDRMR"
BASE COUNT          879 a          947 c         1055 g          852 t
ORIGIN      
        1 ggtatcgatc gtggccgtca atgcccttgg gtgagcacaa gactcgtccc gtcagaacac
       61 aaacaaaaaa gccacggggc tgagcggcac ccgtggcttt cgatgatcaa ttgaaaacga
      121 tcagcgatcg gtggcaggcg cacccagcaa gtattcgaca acttcaacca tgattgtatt
      181 ttgcatgctg cgcctccttt cgtaagatgg gcagagttta acacactgcg agcttttgtc
      241 aagaactaat ttgaatctcg tcgaatgatg gggaagtaat aaatgtacgg cggtgcaatc
      301 gccgtgctgg tcaacgtcgt tgtcactgct cgctgggcat cgctttgcaa acgaacattg
      361 atcgatgcca cgtcggaaat gttaaacgtg atcgagacgg gcaattgaac gaaagcagtt
      421 atagcagtgc cgctgtttac gcctaaattg ctaatggttg cgggcgtaat ggtaatcggc
      481 caaccggatg tgattgaaat gacgtacgaa ttgctgatat agtcggtgtt ggtgaagcgc
      541 agcgtgtaac ttaccacttg accggcgagg ttcgcctgat gatcggcttc tggtgaaata
      601 cgtgcgccga tgatcgcggt gtaaatatcc aggcggccat agccataaac attgttcggc
      661 acgccgccgc tgctgcaatc tgtggttgaa atgtgcacgg cagaattgtt cagcacttgc
      721 tccgtcgccg tgatctgatt cttgagggcc ggtcgggctg accatagcag cgcgatcgcg
      781 cccgcgacat gcggcgtcgc catgctcgtg ccgctcatcg atccatatcc attgccgggc
      841 acgctcgatc gaactggatt gcccggcgca gctaaatcgg gtttgatgcg ctggctacca
      901 tcgatcgtca ccggcccgcg gctgctaaat gaagcgatcg agtctgagcc ggtgttcaat
      961 gcaccaacag tatagctggc atcgtataat gcgatcggct cactaatcgt tgagcaccca
     1021 ggcccatcat tcgtcgcggc agcgatagtc atgatacccg ccgcgcgctg cgcttcgacc
     1081 gcagcttgca aggtattaaa cgagcagcct tctgatgttg gacaactcca tgagttagtc
     1141 gttatatcgg gcgcgagggc gggcaggcct tgtgtgtaag tttctgtgac aggatatggt
     1201 gctaaaaaga attggaaaca ttctaaataa gttgccggtg tgccgtcgcc gacatccatg
     1261 ttgcggcagc cgatccattt tgcgccgggc gccacgccga tctggttccc cgcgccatca
     1321 tcgccgatag ccgtgccggt ggtatgcgtg ccgtgtccgt aatcatcgca tggagttggc
     1381 gaatcaaccc cgcaggatga tccatttgag gttgaatgaa tactatcgtg ccaattgtaa
     1441 tcaaacgtcg tcgtgattcc attccagcca cgaaaatgtg gctgcaaggc aggatgatcc
     1501 cattgcatgc cggtatcctg attgccgatg acgacgcctt gcccggtgaa gcctaaagcc
     1561 cagacttgcg gcgcgtgaat gtaatcgacg cccggttcga tcgaactgat cgaagagggt
     1621 gtagattgat cgggagtaga ggccgatcgg ggcagtggat tgtggatcga tggattgcct
     1681 tcgatccgcg ctacatctga tcgatcagct agcttcagcg cgagatcgcg atcgcccgtc
     1741 accaaaatcg cgttgacgat atagaacgat tgatattgga tatgctgcac agcaagatag
     1801 gcgagcagag attgttgagt ggtttgcgct ttatttagga gggcctgata cacaaactgg
     1861 cctttatcga ttttgctgct cagttgatgg gctggcgata gatcggcttg atcgttgagg
     1921 acgaccaaga actcggcctg tgcgccgcgg tcggtgtgat cgatcaccca gggtgcgatc
     1981 ttactcggcg caagcgttga agcgacgcct gtttcattgg ccggcgcaac atcgatcggg
     2041 cgagcgaggc caatcgaggt tgcaccgatc agcgcagcgg acatgatcga aacaaggagt
     2101 aatggttttc ttggcatggc tcaattgtaa caggcttttt caagtgatcc agtcatttga
     2161 cattgggtta tcaaatgtgc tacccttagc ggggatcaca gaggagtgcc atgcatcgcg
     2221 aacgaatttc agacgacatc tatgttttta ccagcggctt gtacgcgcag gtgaccgccg
     2281 gggccatcat caccagcgct ggcgcggtca tcatcgatac cttgccgttt ccagccgaga
     2341 cacggcaggt catcgactac gtcgagcgcc gatcgccgca aggcattcgg tttgtcatct
     2401 atacgcatta tcatgccgat cacacgtatg gcggttatct ctttaagaac gcgcaggtaa
     2461 tcagtcatca cttatgccgc cagctgttga tcgagcgtgg cgaagccggt atggagttgg
     2521 cgcgcgctca aaatccgcag ttggcggacg tgaagctgcg tctgcccgat attgtattta
     2581 acggcagcct gactttgcgg ctcggaaaca aaacgatcga attggcgcaa tcgcccggcc
     2641 attcgccgga catgatcacc gcttacgtga aagaggatag gattttattc gcctccgata
     2701 ccgtgatgcc ggtgccctat atcgtcgacg gcgataccaa ccagatggct gcatcgctca
     2761 cgaaattgaa gagcatgagc ctggaaaatg tggtgcaggg gcatggcgaa attgtactgc
     2821 gaggcgaaat cagcgaagcg gtgaacagca gtttgaatta tttgcaaaag atgcagggtg
     2881 ctacggcgaa tgcgttgttg ctcaatcaat cgcgtgatga tttgatccgc gagtatgaca
     2941 ttgagtcgtg cggcaaagcc cgcacttcgc taaacgggtt ggtgcagcaa ctgcacgcgg
     3001 cgaatttgat cacaacctat gatcgattga atgatgcacg cacgcacggc ggcactaatc
     3061 acaagctgcg cgcgttggtg cggcttgcgg ttacgaagcc cgtgcgtaaa gtaaagggta
     3121 agcgcaaagg cgctgccaag aaaaccacag ctcgatcgag tgaagctgct ttgaagtcca
     3181 agacgggtaa agcggctaac tcccgttcgg ttaccaagcc cgcgcgttat cgatgacctc
     3241 ctcacgagtc gatgcacttc taaagaagat cgatcaactc aatgcggatg agcgccgcga
     3301 acttctagcg cgcttgcaag ccgaccaact tgcgctgccg tttgttgcgt cggctgaatc
     3361 ttttcaagcc aataccctga tcggtcaacc cgacaccatc atcttgttcg atgggggcag
     3421 ccttggcaat ccgggcaagg gttacggcag ttacgcgtta aaaaataacc attcgaaagt
     3481 ttctcttcaa actagaacga aatctaagga acctgcgcaa ggttggcaga tcacgcgtct
     3541 caacttcggc gataatacaa ctaataacga agccgaatat gctacgttga tgcaggcgct
     3601 cgacgatctc cacagccgca tcgaacgcgc tgatcgattg ccgcaggaat tctcgatcga
     3661 gattcgcggc gacagtacgc tggtgatcaa tcaactgtcg ggcgagtgga agtcgaaaga
     3721 tgatcgcatg cgc
//
LOCUS       DASRTJ010000098         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_80963,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000098 DASRTJ010000000
VERSION     DASRTJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80963"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VFF70_01990"
     CDS             complement(<1..121)
                     /locus_tag="VFF70_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43493.1"
                     /translation="MSIHFGTDGWRAVISEDFTFDNVRRVAQAIADFVVEREGA"
     gene            complement(199..1530)
                     /locus_tag="VFF70_01995"
     CDS             complement(199..1530)
                     /locus_tag="VFF70_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZY43494.1"
                     /translation="MTDHLRSVAALLQQAALEAVEPAEAVYRVLTRVGNSILVNDQAY
                     DLTSFDRVVIVGAGKASIPMADAVAEVLRDRLTGGVVITKYQHTDRSLHPSIRVHEAG
                     HPVPDQNSINGARGIQDRLSDLTARDLIFCVLSGGASALMTLPAENISLEDLQAVTKL
                     LLNAGASIDQVNAVRKHLDLIKGGGLARMAGEATLISLVLSDVVGDDLSVIASGPTAP
                     DRSTFADAWRVIERLELIDRLPQSVRSRLELGTANRIADTPKPGDPLFVNAQTVIVGS
                     NRLAAGAAETLAKKLNFNTLLFSTFVQGEAREVAKVLGAIAKEIIAAERPIKRPACLI
                     WGGETTVTVKGQGQGGRNQELALAAAIAIDGLPNVLIEAFGTDGTDGPTDAAGAITTG
                     ETLTRARDLKLDARSYLANNDAYHFFQPLDDLIFTGPTGTNVNDLMFLLIE"
     gene            complement(1623..2915)
                     /locus_tag="VFF70_02000"
     CDS             complement(1623..2915)
                     /locus_tag="VFF70_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZY43495.1"
                     /translation="MFRIIRFIFSALVVGLILSACTTQPIQVTASAPTPTPIGQPAPS
                     VTPTPSSSPQPNGPITLTLWVPPAYTPGDASPAQKILAQQLQSIDTHVQILTKNDHGA
                     GGLLDLLTAASPVAPSILPDVIALDTTDLEAAARSGLVQPIDDLVPPDLASDLYPFAR
                     DLGSIDGKLYGIIYGADLEHLVFNTNAIKSPPSKWSDLTTQRYVFALHDGDNGVSDAV
                     LSHYLAAGGTLQEANQQPALDQDSLTQLLGLYQQAQRNNILAVNALDLNSDTDVWTAW
                     LASNAALATVHASTYLSVAPALPALQFAALLAIDQPAPPIARGWVLAIVTRDPRRQVA
                     ALKLIEALLSPDRSGVWTQTAKVLPGRVSVLAQWDQTNPYTRFIGDQLQRALAAPSTS
                     IMNVVGPVLRTTIDDVLSNRATPIDAAQAAVNTVNGNK"
     gene            3124..4104
                     /locus_tag="VFF70_02005"
     CDS             3124..4104
                     /locus_tag="VFF70_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZY43496.1"
                     /translation="MSTRKLISVVSLLVSVMLLASACAPAAMATPAPRLQVITNSESA
                     APQAMPAAVPEAPSSGAANSDQSATTSIKNPAGIAVSPYGANRMIIKNGEMTLLVANP
                     QRAIDQVVNVAVTSGGYVVGSKSWTQDNFTYASITMGVPVDQFEAAQRQLRALAIEVQ
                     SDTASGQDVSDQFVDLQSQLTNLEATEARIRQFLDKATKVEEALAVNKQLTEVQGQIE
                     QIKGQMQYLKDRSAYSTIAVQLNPQIPTPTPTATPTATPTATPVAWYPEKTFTQAAGT
                     LNDVMRSLGDLLIWLGVVIVPIVVPIVIVVLIVNRVRRKNVKVSSPKPEA"
     gene            4321..>4607
                     /locus_tag="VFF70_02010"
     CDS             4321..>4607
                     /locus_tag="VFF70_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZY43497.1"
                     /translation="MSTAKPVYIGTDGGATTSKVSGVWSDGTPISISLLQRSTNSQLG
                     PEAVVRGWVEAITEYLGQNQLTWDQVQGVGLSIPGVFRRYGVLDRSANFPAV"
BASE COUNT         1008 a         1207 c         1383 g         1009 t
ORIGIN      
        1 gcgcgccctc tcgctcgaca acgaagtcgg cgatggcctg cgcgacgcgg cgcacattat
       61 caaaggtaaa gtcttcgctg atgacggcgc gccagccgtc ggtaccgaaa tggatggaca
      121 aattgtttac tccaatgata agttttcaat ttgaaaagta aagagtgaag agtgaaaccg
      181 gaatcactcg tcacttattt attcaattaa caagaacatt aagtcattga cattcgtgcc
      241 ggtcgggccg gtgaaaatca gatcgtcgag cggctgaaag aaatgatacg catcgttgtt
      301 cgccagataa gatcgagcgt caagcttcaa gtcacgcgcg cgcgttaaag tctcaccggt
      361 agtaattgcg ccagcggcat ccgtcgggcc atcagtgcca tccgtgccga acgcttcgat
      421 caaaacgttg ggcaagccat cgatcgcgat agcggccgct aaggccagtt cttgattgcg
      481 gccgccttgg ccttgacctt tcaccgtaac agtcgtttcg ccgccccaga tgaggcaggc
      541 ggggcgtttg atcggccgct ccgcagcgat gatctctttc gcgatggcgc caaggacttt
      601 ggcaacttca cgcgcctcgc cttgtacgaa agttgaaaag agcaatgtgt tgaagtttag
      661 tttcttcgcc aaagtttcag ccgctccagc cgccagtcga ttgctgccaa cgatgacggt
      721 ctgagcgtta acgaataacg gatcgccggg cttgggtgtg tctgcaatcc ggttagctgt
      781 gccaagttcc aatcggcttc gcaccgattg cggcagtcga tcgatcagct caaggcgttc
      841 gatcacgcgc cacgcatcgg caaatgtcga tcgatcgggc gcagtggggc cggaggcgat
      901 gaccgataga tcatcgccga cgacatcgct cagcaccaac gagatcagtg tggcttcacc
      961 ggccatgcgc gcaagtccgc cgcccttgat gagatcaagg tgctttctaa cagcgttgac
     1021 ttgatcgatc gatgcgcccg cattcaataa gagtttggtg acggcctgca aatcttcgag
     1081 cgaaatattt tcggcgggca gtgtcatcaa ggccgatgcg ccgcctgata gaacgcagaa
     1141 gattaaatca cgggcagtta aatcactcaa tcgatcttga atgccgcgcg cgccgttgat
     1201 cgaattttga tcgggtacgg gatgcccggc ttcatgcacg cggatcgatg ggtgcagcga
     1261 tcgatccgtg tgctgatact tggtgatgac cacgccaccg gtcaatcgat cgcgcaggac
     1321 ttcggccacg gcatcggcca tcgggatgga cgctttgccc gcgccgacga tcaccacgcg
     1381 atcgaagctg gtcaggtcat aagcttgatc gttgacgagg atcgaattcc cgacacgcgt
     1441 gaggacgcga tagactgctt cggccggttc gacggcttcg agcgcggctt gttgaagtag
     1501 cgcggcgacg gagcgcagat gatcagtcat gttgattggc gtttcatgac cacaaagaga
     1561 ctaagacacc aagtttaatt tatttttctt tgtgccttag cgtcttcgtg gttattgatc
     1621 gactatttgt ttccattcac cgtattcacc gctgcttgcg ccgcatcgat cggtgtggcg
     1681 cgattggaca agacatcgtc gatcgttgtg cggagcaccg ggccgacgac attcatgatc
     1741 gatgtcgatg gcgcggcgag ggcgcgctgc aattgatcgc cgatgaagcg tgtgtacgga
     1801 ttggtttgat cccattgagc taacacgcta acgcggcccg gcaaaacttt ggcggtctgc
     1861 gtccacacgc cgctccgatc gggtgagagc agtgcttcga tcaatttcag tgcggcgact
     1921 tggcggcgtg gatcgcgcgt gacgatcgct aagacccaac cgcgcgcgat gggcggtgcc
     1981 ggttgatcga tcgcgagcag ggcggcaaac tgaagagcgg gcagcgctgg tgcaacactg
     2041 agatacgttg acgcgtggac agttgccagc gccgcattcg aagccagcca cgctgtccaa
     2101 acatctgtat cgctgttgag atcgagtgca ttgactgcca gaatgttatt gcgctgcgct
     2161 tgttggtaca gtcccaacaa ttgcgtcagc gaatcttgat cgagcgccgg ttgttgattg
     2221 gcctcttgca gcgtgccgcc cgccgccaga tagtgcgaca gcactgcatc actgacgccg
     2281 ttgtcgccgt cgtgcagcgc aaaaacgtaa cgctgcgtgg tgaggtcgct ccatttcgat
     2341 ggaggcgact tgatcgcgtt cgtgttgaag acgagatgtt ccagatcggc gccgtagata
     2401 atgccgtata gtttgccatc gatcgatccg agatcgcggg cgaacggata aaggtccgat
     2461 gcgagatctg gcgggacgag atcatcgatc ggctgtacca aacccgatcg ggcggcggct
     2521 tctaaatcgg ttgtatccag cgcgatcaca tcgggcagga ttgaaggcgc aaccgggctg
     2581 gccgcggtca gtagatcgag caagccgcct gcgccatgat cattcttggt caggatctga
     2641 acgtgtgtgt cgatcgattg cagctgctgg gccagaattt tttgtgcagg cgaagcatcg
     2701 ccgggtgtgt atgcgggcgg aacccacagc gtcaaggtga tggggccgtt aggttgcggc
     2761 gaggagctgg gcgtcggcgt cacgctcggc gcgggttggc cgatcggggt tggggtgggc
     2821 gcgctggctg tgacttggat cggctgggtg gtgcaagcgg agaggatcaa gccaacaact
     2881 agcgcgctga agataaatcg aattatgcgg aacatgctca caccaattga cgtccttgtg
     2941 ggtaaggcgt gaggattata gcatgctcgg cggcggttcg gttgtcgctg caaccggata
     3001 gagtagattg ccaatatatt aatgttagtc gagctagatc tgatctataa tcgactggac
     3061 attcgatcga gtcgccgggc tgtgcaattt ctacgcgcta ttcactttcg aggaggaaga
     3121 gaaatgtcta cccgcaaatt gatttcagtt gttagtctgt tagttagtgt gatgttgttg
     3181 gcctcagcgt gtgcaccagc ggccatggcc acacctgcgc cgaggttgca ggtaataacg
     3241 aatagtgaat cagcagcccc acaggctatg ccggctgccg tgcctgaagc accttcaagc
     3301 ggcgcggcga attccgatca aagcgcaacg acttcgatca agaatccggc tggcatcgca
     3361 gtttcgccct acggcgctaa tcgcatgatc atcaagaacg gcgagatgac gctgttggtc
     3421 gccaatcccc agcgggcaat cgatcaagtg gtcaatgtcg cggtgacttc cggcggctat
     3481 gtcgtcggca gtaagtcgtg gacgcaggac aatttcacct acgcgtcgat cacgatgggc
     3541 gtgccggtcg atcagtttga agcggcccag cgtcaactgc gcgcattggc aattgaagtg
     3601 cagagcgata ctgcttccgg tcaggatgtc agcgatcagt ttgtcgatct acaatcgcag
     3661 ctgactaacc tcgaagcgac cgaagcgcgc atccggcagt tccttgataa agcgaccaaa
     3721 gtcgaagaag cgctggcagt caacaagcag ctgaccgaag ttcaaggcca gatcgagcag
     3781 atcaaaggcc agatgcagta tctcaaagat cgatcggcct actcgacgat cgcggtgcag
     3841 ttgaatccgc agatccccac gccgacgccg acggccactc caacggccac gccgacggcg
     3901 acacccgtcg catggtatcc agagaagacc tttactcaag ccgccggaac gttgaacgac
     3961 gtgatgcgct cactcggtga tctgttgatc tggctcggcg tggtgattgt cccgatcgtc
     4021 gtgccgatcg tgattgtcgt gctgatcgtc aatcgagtgc gaaggaagaa tgtgaaagta
     4081 tcgagtccga agccagaagc ataatcaaat gaaggggcga ggtgacctcg cctttactaa
     4141 ccgcagaggc gcggagacgc agagatttca aatgcggctc cgcgcttttt tgcgcctgac
     4201 agttgtcgaa tcagcttgat gcgcgtatac ttcggggctg tacgatcgaa gtccggcttg
     4261 ttccccagtg ctgttacacc atcggataat ggttggccga aaaaattggc aggagacagc
     4321 atgagcacag cgaaacctgt ttacatcggg accgatggtg gcgccaccac ctccaaggtg
     4381 agtggcgtct ggagtgatgg cacgcccata tcgatcagct tgcttcaacg atcgactaat
     4441 tctcaactcg gccccgaggc cgtcgtgcgc ggctgggtcg aagcgattac cgaatatctg
     4501 ggacaaaatc agctgacatg ggatcaagtg cagggtgtgg ggctatcgat tccgggcgtc
     4561 tttcggcgtt acggtgtact cgatcggtct gctaatttcc cggcggt
//
LOCUS       DASRTJ010000099         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_232503,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000099 DASRTJ010000000
VERSION     DASRTJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232503"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..418)
                     /gene="rpsP"
                     /locus_tag="VFF70_02015"
     CDS             complement(11..418)
                     /gene="rpsP"
                     /locus_tag="VFF70_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZY43498.1"
                     /translation="MLRIRLRRIGAKKQAAYRMVIAENEWARDGRFLEIVGQYNPRTD
                     PSTIEVNEDRVLHWLSNGALPTESVGKLLTKVGTLDRFSRLKKGESRETLLAEAKTAA
                     DALRTSATRQTRTNKAAPSKKNRIKQTEAAPAA"
     gene            complement(600..1928)
                     /gene="ffh"
                     /locus_tag="VFF70_02020"
     CDS             complement(600..1928)
                     /gene="ffh"
                     /locus_tag="VFF70_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZY43499.1"
                     /translation="MFDTLTSKLEAIFKKLARHGKLTEADVDEALREVRLALLEADVN
                     FKVVKEFLARVRVRAIGVEVSEALDPAQQVIKIVHDELLETLGTAVPIDLSGSVPHVV
                     MLVGLQGSGKTTTAAKLALKLRSLGQRPLLVAADTYRPAAVTQLETLGKQLNIPVYSQ
                     GVEAAPPEICANGLKLAFEQSYSVVILDTAGRLAIDDQLMSELEAIKKIAKPREILLV
                     ADAMTGQDAVRTAEEFHRRVNITGLILTKVDGDARGGAAISIRSVTGVPIKFLGVGEK
                     SDALEVFHPDRLATRILGMGDVLTLIEKAEANFDQEQATQAAEKMLSARFDMEDFLAQ
                     LQQIKKLGPITGLLDMIPGMGGMKQQIDPHEAEQSLKRIEAIINSMTVKERRQPDLLN
                     GSRKRRIAKGSGTQVQDINQLIKQFRDMQRMMKQLSSGKGRGLASMLRGR"
     gene            complement(2017..>2665)
                     /locus_tag="VFF70_02025"
     CDS             complement(2017..>2665)
                     /locus_tag="VFF70_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43500.1"
                     /translation="RLQGPPGQQIAADVVALIPECGPNDHPDLSGSSVPSCLPAGIDR
                     GQVTDYVASAISSAAADIPDTLDVGPDFVGSGRWSSLITGRSWIRLLDVGILLLVAIT
                     LLIWFIGALIAGHNFKDGLRWAGGWLLGGSIIVLGLSVLMLIGRDAVLSSASFVRSAN
                     DNITPATLDAIRNVASNFIQQFAVRSIIPAGILFALSFVLIVLGRSNSSRTSPSM"
BASE COUNT          575 a          792 c          690 g          608 t
ORIGIN      
        1 gctcacgata ctaggccgct ggggcggctt cggtctgctt gattctattc ttcttgctgg
       61 gtgcggcttt attagtgcga gtctggcgcg tggcgctcgt gcgcagcgca tcggcagccg
      121 ttttcgcttc ggctaacaat gtttcgcgcg attcgccttt ctttaagcga gaaaaccgat
      181 cgagtgtgcc gaccttcgtc agcagtttcc cgaccgattc agtcggcagt gcaccgttgc
      241 tcaaccaatg cagcacacga tcttcattca cttcgatcgt ggacggatcg gtgcgcggat
      301 tatactggcc gacgatttcc agaaaacggc cgtcgcgcgc ccattcgttt tcggcgatga
      361 ccatgcgata agccgcttgt ttcttcgcgc cgatacggcg cagtcgaatt cgtaacacgt
      421 gatgcgctcc ttgatttgta attggataaa tttgtaattg gatgctggag aagagagggt
      481 gaggtgatag ggaccccaac gccaacttca aacttccaat tctcgaccac cattatacat
      541 cattgcctgc ggagtgagag atgtcattta aatagacctc gatattatcc gtcggggtct
      601 tatctgcccc tcaacatcga agcgagccct cggcctttgc cgctcgataa ctgcttcatc
      661 atccgctgca tatcacggaa ctgtttgatc aactgattga tgtcctgcac ttgcgtgccg
      721 ctgcccttgg cgatccggcg cttgcggctg ccgttgagca gatcaggttg ccgccgttct
      781 ttcaccgtca tcgagttgat gatcgcttcg atgcgcttga gtgattgctc ggcttcatgc
      841 ggatcgatct gctgcttcat gccgcccatg cccggaatca tatcgagcag cccggtaatg
      901 gggccgagtt tcttgatctg ctgcagctgc gccaaaaaat cttccatgtc gaagcgcgcg
      961 ctgagcattt tctcggcagc ctgggtggcc tgctcttgat cgaaattcgc ttcggccttt
     1021 tcgatcaagg tgaggacgtc acccataccc agaattcggg tagctaaccg atcgggatgg
     1081 aaaacttcca gcgcatctga tttctcgccc acgcccagga acttgatcgg cacgccggtc
     1141 acactgcgga tgctgatcgc cgcgccgccg cgcgcatcac catcgacttt ggtgagaatc
     1201 aggccagtga tgttcacacg ccgatggaat tcttcggcag tgcggaccgc gtcctggccg
     1261 gtcatcgcat cggctacgag cagaatttcg cgcggcttgg cgatcttttt gatggcttcc
     1321 agttcagaca tcaactgatc atcgatcgcc aatcgaccgg ccgtatcgag aatcaccacc
     1381 gaatacgatt gctcgaacgc cagcttcaag ccgttcgcgc aaatttccgg cggcgcagct
     1441 tcaacgccct gactgtagac cggaatattg agctgcttgc ccaacgtttc caactgcgtc
     1501 acagccgcgg gtcggtaggt atccgccgcg accaacagcg gtctttgacc caggctgcgc
     1561 agcttcagcg ccaacttggc cgccgtcgtc gtcttgcccg agccttgcag gccgaccaac
     1621 atgaccacat gcggcaccga tcccgacaga tcgatcggta cggcagtgcc cagcgtttcc
     1681 agcaactcat catgaacgat cttgatgact tgctgtgccg gatcgagcgc ctcgctgact
     1741 tcaacgccga tcgcccgcac gcgcactcgc gccaaaaatt ctttgacgac tttgaagttt
     1801 acatcagctt caagcaaagc caaacggact tcacgcagcg cctcatcgac atcggcttcg
     1861 gtcagcttgc cgtgccgcgc tagcttctta aagatcgctt ctaatttgct ggttaaagta
     1921 tcaaacattt ctgctccgcg aataacgagc gcagattgta tctgacgcgc tgggattcgt
     1981 caaggaactc acatgaatct atcgtaatat tgtgtcttac attgatggtg atgttctcga
     2041 tgagttcgat cgccctaaca caatcaacac aaacgacagc gcgaacaaaa ttcctgccgg
     2101 gatgatcgat cgaacggcga actgttggat aaagttcgaa gcgacattgc ggatcgcgtc
     2161 cagcgttgcg ggcgtgatgt tatcgttggc cgatcggacg aagctggccg atgagagcac
     2221 cgcatcgcgc ccgatcaaca tcaagacgct caagcccaac acaatgatcg atccgccgag
     2281 caaccagccg ccggcccaac gcagaccatc tttgaaatta tggccggcga tcagcgcacc
     2341 gatgaaccag atcagcagcg ttatcgcaac cagcagcaaa ataccaacat ccaacaatcg
     2401 aatccaactt cgacccgtga tcaatgagct ccaccggcca ctgccgacaa agtccggccc
     2461 gacatcaagc gtgtccggaa tatccgcagc cgcgctgcta attgcgctcg caacgtaatc
     2521 ggtgacttga ccccgatcga ttccagccgg cagacaggac ggcacgctgc tgccagacag
     2581 atcgggatga tcgtttggac cgcattcggg aatgagcgct accacatcgg ccgcgatttg
     2641 ctgaccgggc ggaccttgca atcga
//
LOCUS       DASRTJ010000100         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_308336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000100 DASRTJ010000000
VERSION     DASRTJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFF70_02030"
     CDS             complement(<1..270)
                     /locus_tag="VFF70_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43501.1"
                     /translation="MQQYEAFTSNIGTTIRAEYLPRTVIPRPYTGPALIDPSLPPQAI
                     AANGVATTTQLSRLSNQQVWQVTVISNAATLDFPLLYWPGWIASID"
     gene            complement(334..1575)
                     /locus_tag="VFF70_02035"
     CDS             complement(334..1575)
                     /locus_tag="VFF70_02035"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HZY43502.1"
                     /translation="MSLVIVNDSLTTSSPPRSVRSINWIGLTLLLLSLLAALPLWTGT
                     GLVNTRGGGDSPFLFFRLQQLVTNLHDGVFPARWMPDAAYGLGYPFFNYYASLPYYFA
                     SIFNLLGFDLLISIKIVQTLGFVLAACGMFRWADRHFNSRWISWLIAIAYLYAPFHFV
                     NVYVRGDSLSEFYAFVFYPLILISIDRVVESPRSFHWLALAYGGLILTHNVSALIFAP
                     FALVYAAIRIPHSAIKKSIMALTLGFLLAFALSAWFWLPALGESNLAQLDAQTTGYFN
                     YAEHFRTIDLVQASIGFDYSIATAPGSNTPFAMGLVQALVTLLGLIALVVTWRRDQNK
                     SLRVFALIGLGLSTLMLTPLSRMVWDHLPLLPFAQFPWRFLSIQALFTSLIIGYAVSI
                     IPARRWVFVIFNLLVGSILVA"
BASE COUNT          492 a          427 c          487 g          299 t
ORIGIN      
        1 atcaatcgat gcgatccagc ccggccaata gagcagcgga aagtccagcg tcgccgcatt
       61 cgaaatcacc gtgacttgcc aaacttgttg attcgacaaa cgcgagagtt gggttgttgt
      121 agcgacgccg ttggccgcga tcgcttgcgg cggcaatgat ggatcgatca acgcgggtcc
      181 ggtgtacggt cgcggaatca ccgtgcgtgg cagatactct gcccggatcg tcgtgccgat
      241 gttcgacgtg aatgcttcgt attgctgcaa tcgatccggc gtgatctcat cggcgcgaat
      301 cggtaaataa tctggctgaa gcggtaagag tatttaagca actaatatcg atccaacaag
      361 caaattgaaa atgacgaata cccaacggcg agcgggaata attgaaacag catagccaat
      421 gattaaggat gtaaacagcg cctgtatcga taagaagcgc catggaaatt gcgcgaacgg
      481 cagcagcggt agatgatccc acaccatgcg tgatagcggc gtaagcatca aggtggataa
      541 accgagaccg atcagcgcga agacgcgcag tgacttattt tgatcgcgcc gccaggtaac
      601 gacgagagcg atcaaaccta gcagcgtgac caatgcttga accagaccca tggcaaacgg
      661 cgtgttcgaa ccgggcgcag tcgcgattga gtaatcgaaa ccgatcgagg cttgcacgag
      721 atcgatcgtg cggaaatgtt cggcgtaatt gaaatagccg gtcgtctgcg catcaagctg
      781 tgccaagttc gattcgccca aggccggcag ccagaaccat gcggagagcg cgaaagcgag
      841 aaggaagcca agtgttaaag ccataatact ttttttgatc gcggaatgtg gaatgcgaat
      901 agcagcatag acgagagcga acggcgcgaa gatcaacgcg gagacattgt gcgtcaggat
      961 caagccgcca taagctaacg cgagccagtg aaatgatcgc ggtgattcga cgacgcgatc
     1021 gatcgagatc aagatcaacg gatagaacac aaaggcgtaa aactccgaca gcgaatcgcc
     1081 gcgcacgtaa acattgacga agtgaaacgg cgcgtacagg taagcaatcg cgatcagcca
     1141 gctgatccat cgggagttga aatgccgatc ggcccagcgg aacattccac aagcagcgag
     1201 cacgaagccc aacgtctgaa cgatcttgat cgagatcaat aaatcaaagc cgaggagatt
     1261 gaagatcgat gcaaagtaat agggcagtga agcgtagtaa ttgaagaacg gataaccgag
     1321 gccatacgcg gcgtccggca tccagcgcgc ggggaacaca ccgtcatgca gattggtgac
     1381 gagctgctgc aatcggaaga acaagaacgg actgtcgccg ccgccacgcg tgttaaccag
     1441 accggttcca gtccacaacg gcagcgcggc caacagcgac aataaaagaa gcgtcaggcc
     1501 gatccagttg atcgatctga cgcttcgcgg tggtgaagaa gtggtcaggg agtcattcac
     1561 aatgacgagt gacaagtgat tagtgaaagg tgaatcagcg atacgcgcta tgcgatacgc
     1621 tatctgctat ttcgcgcgcg atcgctgcac gaacatgccc gccataaatc cggcaaccag
     1681 cagaatgacc acgcctaaac caccc
//
LOCUS       DASRTJ010000101        17766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_308396,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000101 DASRTJ010000000
VERSION     DASRTJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17766
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308396"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFF70_02040"
     CDS             complement(<1..672)
                     /locus_tag="VFF70_02040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZY43503.1"
                     /translation="MAERVAIYLQDAHEIREGMKLAQYAESRGFEAVWQAESRLVRDA
                     IVPMAAFAAVTDRIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRIICGIGAWWDPLA
                     RDVGIERKKPMLAIRETIEVMRRLLRMENVTFHGEFHNVTGIELDVVHGRREPRNVPM
                     YIGATGDAMMEMTGEIADGVVLNYCVPPGYNDNALRLLERGAKKVGRNWLDIDRPQLV
                     VCSVDN"
     gene            complement(712..1359)
                     /gene="cofC"
                     /locus_tag="VFF70_02045"
     CDS             complement(712..1359)
                     /gene="cofC"
                     /locus_tag="VFF70_02045"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZY43504.1"
                     /translation="MSSAFSLRSSTFAIIPVKTLRRSKSRLARALKAPVRAALTKSIL
                     SRTLDLLQQVDRITHTIVISNDITVLDLARSKQAIDLVESESGLNAAIKQAATWSVDR
                     GAGSILIVPSDLPLITSADIESMLDLAVELRCVVIAPDRRGEGTNALLVRPPDAIDFA
                     FGAASFHTHTSQSKDLGLAVHVYQSTTIGLDLDIPEDLERYQNSAALLDPLLLSK"
     gene            complement(1438..2421)
                     /gene="cofD"
                     /locus_tag="VFF70_02050"
     CDS             complement(1438..2421)
                     /gene="cofD"
                     /locus_tag="VFF70_02050"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZY43505.1"
                     /translation="MNIVALAGGVGGAKLAHGLTLTPAPLSLSGRRDRGEGKSLTIIV
                     NTGDDFELYGLHISPDLDTVIYTLAGLANPDTGWGLAGDTFQFLDALKRLGVEMWFRI
                     GDRDLATHIRRTELLRQGKTLTEATQLITQSLGITSMILPMTDDRFRTMLNTDQGRLE
                     FQEYFVHRQWQPRIQSIEFDGASTARLTQPAADAIQVADAIIICPSNPFVSIDPILTL
                     TPAPLSLKRRRDGGENIPIVAVSPIVGGQAIKGPAAKMFEELGLEPAALNVAKHYQGL
                     VTHFVLDQIDASQESAIQLLGMRTFVTDTLMKSIDDRVRLANEVISFISNT"
     gene            complement(2534..3133)
                     /locus_tag="VFF70_02055"
     CDS             complement(2534..3133)
                     /locus_tag="VFF70_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZY43506.1"
                     /translation="MNLTELIHTRRSIRHYTDQSVPLELINQLLEAASWAPSAHNRQP
                     WRFVVISTPEAKHHLAAVMGERLRADRLRDGDPLEAIERDVARSHDRIVQAPIVIIAC
                     LSMNDMDRYPNDRRTQAERTMAIQGVAMSMQNMLLTAHDLGLGACWMCAPLFCSGEVR
                     SALALPIDWEAQALITIGYPIDQSKSKDRVNFKQKTIYR"
     gene            complement(3216..4805)
                     /locus_tag="VFF70_02060"
     CDS             complement(3216..4805)
                     /locus_tag="VFF70_02060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZY43507.1"
                     /translation="MSDLILYNANIHTIDPAKPTAQSIAIRDNKILALGDDQALHSLL
                     PSAEAIDLKCRCVIPGLIDAHLHWEWVSLGLKNIDCETPTLAEMLRRVAERVRITPPD
                     KWIRGHGWNQNVWGETFPTKTDLDRVALNHPVYLTAKSGHAGLANSIALKLANVTAST
                     LNPTHGEIVRDEHGEPTGVFFEDAMDLVSQKIPQVSAQELANAMRDAISHAHRVGLTG
                     VHDFDGAAALQAWQILKENDQLNLRVSKTIPVYLLDHAIALGIRSGFGDNWLRINGVK
                     TFADGALGPRTALMIDPYIGEPNNRGIAVTDKEEMFENVRKASAAGLSSTIHAIGDRA
                     NHDVLDVYESVRQEEAQRGESRLRHRIEHVQLLHPDDLNRLGQLKVIASMQPIHATSD
                     MLIAEKHWGARSAGGYTWRTQLNAGAMLAFGSDAPVESFNPLLGIHAAVTRRRADGSP
                     AADGWYPDQKMNVTEAVHGFTLGAAYASYEEKNKGSLTPGKLADLVVLNQNIFTIGPM
                     EILNTMVEATMIDGQFVWRNF"
     gene            complement(4890..5600)
                     /locus_tag="VFF70_02065"
     CDS             complement(4890..5600)
                     /locus_tag="VFF70_02065"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HZY43508.1"
                     /translation="MMSASIASTDPVLQRMSSLVTEWEQAHDRRSIFLGCYQLMTANM
                     LGAIQQARFHDDEWVSNLLHRFAKYYFDALELYNLHSDETPQVWRLAHNATRQEKVMT
                     LQHLMLGVNAHINYDLVFTLHDMLAPEWNDLSTEQRQQRLSDHTLVNKIIGETIDTVQ
                     DQVIDQHSPWFSLVDKLLGPTDEWLTSRLIAHWRKEVWTNAIEYVELHDRQARLAFQQ
                     KIEASAMRRGNEFLELTP"
     gene            complement(5681..6880)
                     /locus_tag="VFF70_02070"
     CDS             complement(5681..6880)
                     /locus_tag="VFF70_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZY43509.1"
                     /translation="MSSNQKLILEKVEQAIGILREKDVDLWLTFVRETTQVHDPTLSL
                     ILSEDLQLTWHSAMILTQQGDRIAIVGRFDKENVMLTGAYKEVIGYDKSIRPLLVDVL
                     KRINPDCIAINYSENDPAADGLTHGMWTQLFRLLEGTPYAAKLISAEDVIGALRGRKS
                     KAEVDRVRSAIQETQRIFDAVTGFLRPGQKELEIRDYMHEQLANRGLTTAWEIDFCPI
                     VNGHPETPIGHAAATEHRTDRGHTLQIDFGVMKDGFCSDVQRMWYFLDQGETKPPADV
                     QKGFDAVRAAIEAGAKVLKPGVLGWEVDQAARSTLTKLGYPEYQHATGHELGRSAHDG
                     ATVLGPKWERYGETINGEVEVGNVFTLELGVHLPTRGYISLEEDILITANGLEWLTKP
                     QTQLMCI"
     gene            complement(6886..7740)
                     /locus_tag="VFF70_02075"
     CDS             complement(6886..7740)
                     /locus_tag="VFF70_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZY43510.1"
                     /translation="MKKGGLGRGLDALLPESEALVTSGLTEVSVHSIQPNPHQPRSTF
                     DETTLAELADSIREHGLIQPLIVVRADEDRFTLIAGERRWRAAQIAGLASVPVVIKDA
                     APQQMLELALIENVQRADLNPLEEALAYKQLCEEFGLTQDQVAQRVGKSRVAISNTLR
                     LLKLPDAIKVHLVGNQITEGHARALLMLDDLTLQQRTSNQIIKHGLNVRQTEELVRRL
                     TENRPAKKGQSPNRANADSRALEDRMRRSLGTKVSLFRSKKGGTVVIHFYSEEELDSI
                     YRRITKGD"
     gene            complement(7746..8546)
                     /locus_tag="VFF70_02080"
     CDS             complement(7746..8546)
                     /locus_tag="VFF70_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY43511.1"
                     /translation="MLHIYAIANQKGGVGKTTTAVNLGVYLAAKGQRVLLIDFDPQAN
                     ATSSLGFDKKKITSSMYNVLIEGKPLSEVIQLTKRVRLDLAPSTPQLAGAEVELVALT
                     ERERRLKQAMVQIQDNYDYILVDCPPALSLLTINALAAVQGVIVPVQCEYLALEGLSQ
                     IMNTVQLVRKALNPSLTLRGLLMTMFDSRARLAQQVIAEVRSHFGDRVFKTLVPRSIR
                     LGEAPSFGQPILTYAATSSGAIAYDQLADEVLLGDHTNGEVKPGVSAT"
     gene            complement(8548..9375)
                     /locus_tag="VFF70_02085"
     CDS             complement(8548..9375)
                     /locus_tag="VFF70_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZY43512.1"
                     /translation="MIRYTAIGHITKDIVANGWVFGGAVTFSARAARALGCDVSVVTS
                     CERGLDLISVLPDVAIVYQPSDRTTTFENIYMSNGRVQRLHAISDKLKIDLAQSLPRP
                     IDIVHLAPVAQEVDPAWIDQFDGALIGVTPQGWLRQWDSAGRVKPIEWANAAEILRRV
                     AATIISIEDIGGDESIAQQWAAIAPVLVVTRGKAGCTVFSAGQPIDLAAPAVNAIDTT
                     GAGDIFAAAFFVQLRRTHDPIAAGRFAVCLAAQSTTRVGIDGVPTRTEIEQCLAATQ"
     gene            complement(9461..10132)
                     /gene="jag"
                     /locus_tag="VFF70_02090"
     CDS             complement(9461..10132)
                     /gene="jag"
                     /locus_tag="VFF70_02090"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZY43513.1"
                     /translation="MDTPQSIEAVGSDAENAIARALEALNVRRDQVDVLVLETNPRAT
                     RVRLTVKSDRLQAAASTGTDAEVAERIVAALLDKMHLVVSVSAREAVAQDVEDEQRGP
                     AIEVDVTGDDLSNLIGRRGETLDNLQYVARLLIAKELGRYVNLIVDVAGYKSHRAEML
                     KQLAQRLAGQVVNTHKPASMEPMPANERRIIHLALRDHPGVRTESVGEGERRKVTIVP
                     KSVKP"
     gene            complement(10148..10933)
                     /locus_tag="VFF70_02095"
     CDS             complement(10148..10933)
                     /locus_tag="VFF70_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZY43514.1"
                     /translation="MDFLVSGMVNVLLFFYHLLGNSFVLSIVVATVMVRLITLPLTMP
                     QMRSSRKMAALQPQLAELKKKYGNDKQKFAEAQMKLYRENNVSMFGGCLPLILQMFIM
                     IAFYQAITGALAVNPLQLLSLSHRAAPELASLIPIESKFLWLNLGQSDQTPILMFLLP
                     ALVVVTTYLQQKLAAPPSGDPSAASVSKQMNIMMPMMFGLFALNFASGLSIYFIVSNA
                     IGVLQYMVLGKRMTPAVAAAPAKASIKSATTNTKALAAPKAKK"
     gene            complement(10964..12136)
                     /locus_tag="VFF70_02100"
     CDS             complement(10964..12136)
                     /locus_tag="VFF70_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZY43515.1"
                     /translation="MLKHRWFAIALMVVLMSVLAACGGAPAVTSWPGMVVRDNTAYIA
                     SSDQVYALDVATNVDAKRQVWVIKPVQSTSIGYHSQPAISADGKTLYLGADAITGNSG
                     LVVAMNTENVTVTWTYPLTKTDIDPGNIYGGIVLANEKLFFAGGFGQLFALDAATGRP
                     AWNQPFDPKTNTRIWSTPAVSDKLVFIASQDHHVYAVNQSDGNQAWEFPAQGDQSLTV
                     GTFAGSPAVYSDTIYVGSFDSNLYALDINGKLKWKFTATGRLWDPPAESDGTLYFGDL
                     SGNVYALDAATGQSKWPQSVTVIGGVRATPLIADGKVFIGTDQFKMYALDTATGRAVW
                     QNPFSGKDGEMFIVTPAIYSTTLVALPNLAGGTPTRLYGLNAETGAQLFVFPPPAP"
     gene            complement(12160..12369)
                     /gene="yidD"
                     /locus_tag="VFF70_02105"
     CDS             complement(12160..12369)
                     /gene="yidD"
                     /locus_tag="VFF70_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZY43516.1"
                     /translation="MKTIFLFLIRGWQLTFAKVLPPSCRFEPSCSHYGYEAIQKYGAL
                     KGGWLTLKRIGRCQPFHPGGYDPVP"
     gene            complement(12366..12740)
                     /gene="rnpA"
                     /locus_tag="VFF70_02110"
     CDS             complement(12366..12740)
                     /gene="rnpA"
                     /locus_tag="VFF70_02110"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZY43517.1"
                     /translation="MARVFRLRPHGEFDRVRQNGRNWSHPMLIIIAQTRTASPIDRAR
                     VSVATGKKLGGAVVRNRVKRKLRAAVQQVYPHLRTTIDLIVIARQPMLEASVIDITQA
                     LTSTLRRAQLWLEDSSAEDQDR"
     gene            complement(12788..12964)
                     /gene="rpmH"
                     /locus_tag="VFF70_02115"
     CDS             complement(12788..12964)
                     /gene="rpmH"
                     /locus_tag="VFF70_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZY43518.1"
                     /translation="MPKRTFQPRKRRTARTYGFRARMKTKGGRKVLQARRLKGRHSLA
                     YQPSRAKRIDWKAG"
     gene            complement(13150..14439)
                     /gene="murA"
                     /locus_tag="VFF70_02120"
     CDS             complement(13150..14439)
                     /gene="murA"
                     /locus_tag="VFF70_02120"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZY43519.1"
                     /translation="MDQFVIEGGQPLTGQTTPSGNKNAALPLMAACMLTDQPIILHNV
                     PRIRDVRTMLQLLADLGVDISEPDGHTLHLRAANVRKTKLDPDLCREIRASILLAGPM
                     LARLKSIELSPPGGDVIGRRRVDTHFLALTRLGAEIEINHEFRMKADRLVGCDILLDE
                     ASVTGTENAIMAAVMAHGTTILRNAASEPHVQELCLMLNQMGAQIDGIGSNTLTIHAV
                     STLHGAEFTIGPDYLEIGSYIGLAAITGGEITIHNSAPRHLSMVRLVFERLGVKFETR
                     GDDLFVGREQSLTVVPDIGGAIPKIDDAPWPAFPADMMSTALVMATQAAGSVLIHEKM
                     FESRLFFVDKLIGMGARIVLCDPHRCIVQGPSWLHGERLESPDIRAGMALVSAALCAQ
                     GQSIIRNVGQIDRGYEQIEVKLQSLGAKIYRDTEHRG"
     gene            14731..15657
                     /locus_tag="VFF70_02125"
     CDS             14731..15657
                     /locus_tag="VFF70_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43520.1"
                     /translation="MARHPWLLIVPFALDLFLWLGPRIQASALYDQFVPYLQNFPVAL
                     DVDTRLAVQQFMQALQEFFSKFNLFSWLSSGLFGVPTLNAGGDSTAKLVTGGVPAVWQ
                     ISDVGSYILLIIALNIVGLLAAALLWVMLIDVMRKEVLNLRNMLKRSFSLWVRLLTFV
                     LALLAVFIVLALGLSVVTALIGAISLSFAAFIPLVVLSLGAWGLFYLAFTLHGLALYN
                     LPAVRSMRLSMLMTRLFFAPTLGLVVISIAIYMGIGLIWTNFAIDSWARLIAMLGNAF
                     IVAGLMMSSLIYYQNRSSIVLDFLQSVKPTEN"
     gene            15734..17674
                     /gene="gyrB"
                     /locus_tag="VFF70_02130"
     CDS             15734..17674
                     /gene="gyrB"
                     /locus_tag="VFF70_02130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZY43521.1"
                     /translation="MAEPKAKYDASQIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYE
                     VVDNAIDEALAGEADNIDVNIHADMSVSVRDNGRGIPVGPHPTQKDANGKAMDAVEVV
                     MTILHAGGKFGGSGYKVASGLHGVGVSAVNALSEWLITTVHQDGKEYRQEYKRGKPTG
                     PVKLIGKLAKTETWVRGTDQRFMPDRSIFKDEIDYKFDTLIQRFREMAFLTRNLTIKF
                     IDERDGHEMSFHFEGGVRSFVRYLNKSRAVLHPPVYVDKQINGTAVEAAIQYTDGYTE
                     SVYAFANNINTIDGGTHVTGFRSALTRTINDYARKAGLLKDSDPNFSGDDVREGLTAI
                     ISIKLQDPQFESQTKSKLGNAEVKGQVETAVGEAFTEFLEENPREGKAIITKCLTSSR
                     AREAARQARDLVIRKSALESMTLPGKLADCSERDPAKCEIYIVEGDSAGGSAKQGRDR
                     HFQAILPLRGKILNTERARMDKILANNEVKSLISALGCGIGEQFNLDNLRYHRTIIMT
                     DADVDGSHIRTLLLTFFFRYMQSLVEGGFLYIAQPPLYRIESKKLGVKYVYNDADKDI
                     EVAGLKDEKVSIQRYKGLGEMNPEQLWETTLNPENRTLLQVTVEDAAAADRTFDMLMG
                     DEVPPRKRFIQTHAKEVRNLDI"
BASE COUNT         3979 a         4705 c         4818 g         4264 t
ORIGIN      
        1 gttatcgact gagcaaacga ccagctgtgg tcgatcgatg tctagccaat tgcggcctac
       61 tttcttggca ccacgctcta acaacctcag tgcgttatcg ttgtatccgg gtggcacaca
      121 ataatttaac accaccccgt ccgcaatctc accggtcatc tccatcatcg catcgcccgt
      181 cgcgccaata tacatgggca cgttgcgcgg ctcgcggcga ccgtgcacca catccaattc
      241 gatgccagtg acgttatgga actcgccatg gaaggtgacg ttctccatgc gcagcaagcg
      301 gcgcatcact tcgatcgttt ctcggatggc caacatcggt ttcttgcgtt cgatgccgac
      361 atctcgcgcc aagggatccc accacgcgcc gatgccacag atgattcgat tcggcgcgag
      421 atcgtccagc gttaaaaaag tcgcggccag cagaccgatg ttgcgtgtcc agttgttgat
      481 cacaccggag ccaaccttaa tccgatcggt tacggcggcg aaagcggcca ttggcacgat
      541 cgcatcacgt accagccgcg actcggcctg ccagacggct tcgaagccgc ggctttcggc
      601 gtattgcgcc aacttcattc cttcgcgaat ttcatgggca tcttgcagat aaattgctac
      661 gcgttcggcc attgtttctc ctttgcgagt ggttgattgc taacgagtat cttatttcga
      721 aagcagaagc gggtcaagta gagccgcgct gttttgatac cgctccaaat cctctggtat
      781 atccaggtca agcccgatcg ttgtcgactg atagacatgc accgccaagc cgagatcttt
      841 tgattgcgac gtatgggtat gaaacgatgc cgcgccaaac gcaaaatcga tcgcgtcggg
      901 cgggcggacg agcagggcgt tagtgccttc gccgcgccga tcgggcgcga tgacgacaca
      961 gcgcagctcg accgccagat caagcatcga ctcaatatcg gctgaagtga ttaacggtaa
     1021 atccgacggg acgattaaga tcgatccggc tccacggtct actgaccaag ttgctgcttg
     1081 tttgatagcc gcattcaagc ccgactcgga ttcaacgaga tcgatagcct gtttgctgcg
     1141 tgcgagatcg agtaccgtaa tatcgttact gatgacgatg gtgtgggtga tgcgatcgac
     1201 ttgctgcaat agatcgagcg tacgcgacaa gatcgatttg gtgagcgcgg cgcgtaccgg
     1261 cgctttcaag gcgcgcgcca ggcggctttt cgatcgacgc agcgtcttga cgggaatgat
     1321 cgcaaaggtt gaagatcgaa ggctgaaggc tgaagacata gtcggttgcg tggtgcgtga
     1381 gatatagatg gagcctatta ccttcggtaa gggcaagcac acaccacttg atctttctca
     1441 agtattgcta atgaaagaga taacctcgtt cgcaagtcga acacgatcat cgattgattt
     1501 catcaaagta tcagtcacga acgttcgcat gcccagtaat tgtatcgcac tttcttggct
     1561 cgcatcgatt tgatcgagca cgaaatgtgt gaccaagccc tgataatgtt tcgcaacatt
     1621 caaggctgcc ggttccaagc cgagctcctc aaacatttta gcggcagggc ctttgatcgc
     1681 ttgccctcca acgatcggcg aaacagcaac aattggaata ttctcacccc cgtccctcct
     1741 ccttttaagg gagaggggtg caggggtaag ggtcaatatc ggatcaatac tcacgaatgg
     1801 attcgacggg cagatgatga tcgcatctgc aacttgaatc gcatcggctg ctggttgagt
     1861 aagccgcgcc gtcgaagcgc catcaaactc gatcgattgg atgcgcggct gccactgtcg
     1921 gtgaacaaag tattcttgaa attccaatcg gccttgatcg gtatttagca tcgtgcggaa
     1981 tcgatcgtcg gtcatcggta agatcattga ggtgatgcca agcgattgag tgatcagttg
     2041 agtcgcttcg gtaagtgtct tgccttgacg cagcagctcg gtacggcgaa tgtgagtggc
     2101 gagatcacga tcgccaattc ggaaccacat ctcaacgccc aatcttttga gcgcatcaag
     2161 aaactgaaac gtatctccgg caagacccca acctgtatcg ggattggcta gaccggccag
     2221 cgtgtaaatg acggtatcaa gatcgggcga gatgtgcaga ccatataatt caaagtcatc
     2281 accggtgttg acgatgatgg tgagactctt accctcaccc ctatcccttc tccctgaaag
     2341 ggagaggggc gcaggggtga gggtcagtcc atgtgccaac ttcgcaccgc cgacgccgcc
     2401 agccagtgcg acgatgttca tcatattctc cagtaggggc gacgcattcc caatagatca
     2461 gtgtcgatcg gcttatcaat tgcgagcagc atgtttagct gctgcgattg atacggaatg
     2521 cgtcgcccct acattaacgg taaattgttt tctgtttgaa attaacgcga tctttggatt
     2581 tgctctgatc gatcggataa ccgatggtga tcaaggcttg tgcttcccaa tcgatcggca
     2641 aagctagcgc cgatcgaact tcgccgctgc aaaaaagcgg ggcgcacatc cagcatgcgc
     2701 cgaggccgag gtcgtgcgca gtgagcaaca tgttctgcat cgacatcgcc acaccttgaa
     2761 tcgccatcgt gcgttcggct tgcgtgcgtc gatcgttggg atagcgatcc atatcgttca
     2821 tggatagaca ggcgatgatc acgatcggcg cttgaacaat tcgatcgtgc gagcgagcca
     2881 catcacgttc gatcgcttcg agcggatcgc cgtcgcgtaa tcgatcggcg cgcaggcgtt
     2941 cgcccatcac cgcggctaag tggtgcttcg cttcgggcgt cgagatcacc acaaagcgcc
     3001 acggctgtcg attgtgcgcc gagggtgccc aactggcggc ttcaagaagt tgattgatca
     3061 attccaatgg gacagattga tcggtatagt gtcggatcga tcggcgggtg tgaatgagtt
     3121 cagtcagatt caaagtttaa ttcctggtga tgagtttgat ttgcactgat aatccagagt
     3181 cgaaaagcga taagcaatct gctatcagcg atcggctaaa aattccgcca gacaaactgt
     3241 ccatcgatca ttgttgcttc aaccatggtg ttgagaatct ccattggccc aattgtaaaa
     3301 atattttggt taagcaccac tagatcagcc aacttgccgg gcgtcaatga tcctttgttc
     3361 ttctcttcgt atgaagcata tgccgcgcct aacgtaaagc cgtgcaccgc ttcagtcacg
     3421 ttcatctttt gatcgggata ccaaccatcg gcagccggtg atccatcggc gcggcggcgc
     3481 gtcacggccg catgaatacc tagtagcgga ttgaacgatt caaccggcgc gtccgatccg
     3541 aatgccaaca ttgcgcccgc attcaactgc gtgcgccagg tgtaaccacc tgcgcttctc
     3601 gctccccaat gtttttcggc gatcagcata tcgcttgtgg cgtgaatcgg ttgcatcgag
     3661 gcgatcacct tcaactgacc gagccgattg agatcatcgg ggtggagcaa ttgcacatgc
     3721 tcgatgcgat gccgcaatct cgattcccca cgctgcgctt cttcttggcg gaccgactca
     3781 tagacatcca acacatcatg attcgctcgg tcgccgatcg cgtggatggt agaggataag
     3841 cctgccgcgc tggccttgcg cacattctca aacatctctt ctttgtcggt aacggcgatg
     3901 ccgcgattgt ttggctcgcc aatataggga tcgatcatca acgcggtgcg cgggccgagt
     3961 gcgccatcgg caaaggtctt gacgccattg atgcgcagcc agttatcccc gaagcccgat
     4021 cggatgccca gcgcgattgc atgatcgagc aagtacaccg gaatggtttt gctcacgcgc
     4081 aaattcaatt gatcgttctc tttgagaatc tgccacgctt gtaacgcagc cgcgccatcg
     4141 aagtcatgta cacccgtgag gccaactcga tgcgcgtgtg agatcgcatc gcgcatcgca
     4201 ttggcaagtt cttgagcgga tacttgcgga attttctggc tgaccaaatc cattgcatct
     4261 tcaaagaaca ctccggtcgg ttcgccgtgc tcatcgcgaa cgatctcgcc gtgagttgga
     4321 ttcagcgttg acgcagtgac attcgccaac ttcaacgcga ttgagttcgc gagtccggca
     4381 tggccgctct tggccgtgag atacaccgga tgattgagcg caactcgatc gagatcagtc
     4441 ttggttggaa atgtttcgcc ccacacattt tgattccagc catgcccgcg aatccattta
     4501 tcgggtggcg tgatgcggac gcgctcagcc acacgccgca gcatttcagc caacgtcggt
     4561 gtttcgcaat cgatattctt caagccaagc gacacccatt cccaatgcag atgcgcatcg
     4621 atcagaccgg gtatcacgca gcggcatttg agatcgatcg cttcggcgct gggcagcaga
     4681 ctgtgcaacg cttgatcgtc gccgagcgcg agaatcttat tgtcgcggat cgcaatcgat
     4741 tgcgccgtcg gcttcgccgg atcgatcgtg tggatgttgg cgttgtagag aataagatca
     4801 gacatgcctg gctccgatgc gagacgtcgt ttgtcgaaat ctttgatggg ttcgggcgct
     4861 aaacggttga aacgtaaagt gagaatcggt taaggggtta attccaggaa ctcattgccg
     4921 cggcgcatgg cgctcgcttc gatcttttgc tgaaaggcca gccgtgcctg acgatcatgc
     4981 agttcgacat actcaatggc atttgtccag acctctttgc gccagtgagc gatcaagcgc
     5041 gaagtcaacc attcgtcagt cgggcctaac agtttgtcca ctagactgaa ccagggcgaa
     5101 tgttgatcga tgacctgatc ctgaacggta tcgatcgttt cgccgatgat tttgttgacc
     5161 agcgtgtgat cgctcaagcg ctgttgacgc tgctcggtcg agagatcatt ccattcgggg
     5221 gcgagcatat cgtgcagcgt gaagaccaga tcgtagttga tgtgggcgtt aacgcccagc
     5281 atcaggtgtt ggagcgtcat tactttttct tggcgggtag cattgtgcgc cagccgccag
     5341 acctgcggtg tctcgtcact gtgtaagtta tacagttcca gggcgtcgaa gtaatacttg
     5401 gcaaagcggt gcaggagatt agaaacccac tcgtcatcgt gaaagcgtgc ctgttggatc
     5461 gcacccagca tgttggcggt catcagctgg taacaaccta aaaagatgct gcgccgatcg
     5521 tgtgcctgtt cccattcggt gacgagtgaa gacatgcgtt gaagcactgg atctgtagat
     5581 gcgatcgagg ctgacatcat cgattacggt tgaacgtcaa atgtgcgtgg gctcaattcg
     5641 aaatctgaag ggagcaactt gttcaccttt tgacggaaac ctaaatgcac atcaactgtg
     5701 tctgcggctt agtcaaccac tccaagccat tcgctgtaat gagtatatct tcttcaaggc
     5761 tgatatagcc gcgggtgggc aagtggacac ccagctccaa agtgaagaca ttgcccactt
     5821 ccacttcgcc gttgatcgtt tcgccatagc gttcccactt aggaccgagc accgttgcgc
     5881 cgtcgtgcgc gctgcggcct aactcgtgtc cggtggcatg ttgatattcg ggataaccga
     5941 gtttggtcaa tgtcgatcgc gcggcctgat cgacttccca gcctaacaca ccgggcttca
     6001 acacttttgc gcccgcttcg attgcggcgc gcaccgcgtc gaaacctttt tgcacgtcgg
     6061 ctggcggttt ggtttcgcct tggtcgagga agtaccacat gcgctgcacg tccgaacaga
     6121 agccgtcttt catcacgccg aaatcgatct gcaaagtatg gccgcgatcg gtgcggtgct
     6181 cggtggctgc ggcatgcccg atcggggttt cgggatgccc gttgacgatc gggcagaaat
     6241 ctatttccca cgcagtggtc aggccacggt tcgctaattg ttcatgcatg taatcgcgta
     6301 tctcaagttc tttctggccg gggcgcagaa aaccggtaac tgcatcaaag atgcgctgtg
     6361 tttcttgaat cgcgctcctc actcgatcga cttcggcttt ggatttgcgg ccgcgcagcg
     6421 cgccgatgac atcttcagcc gagatgagtt tagcggcgta tggcgtgcct tccaataagc
     6481 ggaacagctg cgtccacatg ccgtgggtca agccatccgc tgccggatcg ttctcgctgt
     6541 agttgatggc aatgcaatcg ggattgatgc gcttgagcac atcgaccagt agcgggcgaa
     6601 tacttttatc gtagccgatg acttctttat aagcgccggt caacatcacg ttctctttat
     6661 cgaagcggcc gacgatggca atgcgatcgc cctgttgggt taagatcatg gccgagtgcc
     6721 aggtcagctg caagtcttca ctcaagatca aactcaaagt aggatcatgc acttgagtgg
     6781 tctcgcgcac aaacgtcagc cacaagtcca catctttttc acgtaagata ccgatggcct
     6841 gttcaacttt ttcgagaatt aatttttgat tactcgacat gctgcttaat ctcctttagt
     6901 gatccgccgg tagatcgaat ccagttcttc ttctgaatag aaatggatca caaccgtacc
     6961 gccttttttc gatcggaaca aactgacttt agtgccgagt gaacgccgca tccgatcttc
     7021 cagtgcgcgg gaatcggcat tcgcccgatt tggactctgt ccctttttag ccggtcgatt
     7081 ttcagtcaag cgccgcacga gctcttcggt ctggcgcaca ttcaagccgt gcttgattat
     7141 ttgattcgac gtgcgttgct gaagggttaa atcgtcgagc atcaacagag cgcgggcatg
     7201 accttcggta atttggttac ccaccaaatg cactttgatg gcatccggca atttcaataa
     7261 gcgcagcgtg tttgaaatcg ctacacgact cttgccgaca cgctgggcca cttgatcctg
     7321 cgtcaagcca aactcttcgc acaattgttt atacgctaac gcttcttcca gcgggttgag
     7381 atcagcgcgc tgcacatttt caatcagcgc caattccagc atttgctgcg gtgcggcgtc
     7441 tttgatcacg acaggcacgc tcgctaatcc ggcgatctgc gcggcgcgcc agcgccgttc
     7501 gccggcaatg agcgtaaatc gatcttcgtc cgcgcggaca acgatcagtg gctgaatcaa
     7561 gccgtgttcc cggatcgagt cggccaattc tgccagtgtg gtttcatcga acgttgatcg
     7621 cggttgatgc gggttaggtt gaatcgaatg cactgaaact tcagttaaac ctgaagtcac
     7681 cagcgcttcc gattccggaa gcagcgcgtc taaacctcta cccaatccgc cttttttcat
     7741 attcatcatg tagcgctgac acctggcttg acttcaccgt tggtgtgatc gcctagcaat
     7801 acttcatcgg ccaattgatc gtaagcgatt gcgccggacg aggtcgccgc gtacgttaat
     7861 atcggttggc cgaatgaggg agcttcaccc aatcggatgc tgcgcggcac tagcgtcttg
     7921 aatacccgat cgccaaaatg tgatcgaact tcggcaatga cctgctgcgc caatcgtgca
     7981 cggctgtcga acatcgtcat cagcaacccg cgcagcgtca aacttgggtt gagcgctttg
     8041 cggacaagct gcaccgtatt catgatttgg gatagccctt ctaaagccag atactcgcac
     8101 tgtactggaa cgatcacacc ctgcaccgct gctaacgcgt taatcgtcaa taagctaagc
     8161 gcgggtgggc agtcaaccaa aatgtaatca tagttatctt gaatctgtac catcgcttgt
     8221 ttaagccgcc gctcgcgttc ggtcaaggcg actaactcga cttcggctcc agcgagctgc
     8281 ggtgtcgacg gcgcgagatc caaacggacg cgcttggtca attgtattac ttcggacagc
     8341 ggcttaccct caatcaatac attatacatc gaggacgtga tctttttctt atcgaagccc
     8401 agcgacgacg ttgcattcgc ctgcggatcg aaatcgatca atagcacgcg ttggcccttc
     8461 gcggccagat acacgcccag attcacggcg gtggtagttt taccgacgcc gcccttttga
     8521 ttggctatcg cataaatatg cagcatgtta ttgagttgcg gctaaacact gttcaatttc
     8581 agtgcgcgtc ggcacgccgt cgatgcctac gcgcgtcgtc gattgcgcgg cgaggcagac
     8641 ggcgaagcgc ccggcggcaa tcggatcgtg cgtgcgacgg agttgcacaa aaaaagcggc
     8701 ggcaaaaata tcgcccgcgc ccgtcgtatc gatcgcgttc acagcgggtg cggctaaatc
     8761 gatcggttgt ccagcgctga aaactgtgca gccagctttg ccacgtgtca cgactagcac
     8821 cggtgcgatc gctgcccact gctgcgcgat cgattcatcg ccgccgatat cttcaatgct
     8881 gatgatcgtc gcagcgacgc ggcgcaaaat ttctgcggcg tttgcccact cgatcggttt
     8941 aacgcggccc gctgaatccc actgacgcag ccagccttgc ggcgttacgc cgatcagcgc
     9001 gccgtcgaac tgatcgatcc acgctgggtc gacttcttgt gcgaccggag ccagatgtac
     9061 gatatcgatc ggacgtggca aactctgcgc aagatcgatc ttcaatttat cgctaattgc
     9121 atgcagcctc tgcactcggc cattcgacat ataaatattt tcaaaggtcg tggtccgatc
     9181 ggagggctga taaacgatag cgacatcagg cagcaccgaa atgagatcga ggccgcgttc
     9241 acagctggtg acgacgctca catcacagcc taacgcgcgg gcggctcgcg ctgaaaaagt
     9301 tactgcgccg ccgaataccc agccgtttgc tacaatgtct ttagtgatat gcccaatcgc
     9361 cgtatagcgt atcattcaat aggacaaatg aaaaatgacc actgacatat taaagcgttt
     9421 agctttttgt catgggtcat tgaatatcgg acattgactt ctagggcttg actgattttg
     9481 gtacaatcgt cactttgcga cgttcaccct cgccgacgct ttccgtgcgg acgccggggt
     9541 gatcgcgcaa cgccagatgg ataatccgcc gttcattcgc gggcataggt tccattgatg
     9601 ccggcttgtg cgtgttaacg acttgtccgg ccaagcgctg cgccaattgt ttcaacattt
     9661 ctgcacgatg tgatttgtag cctgctacat cgacgatcag attcacatag cggcctaatt
     9721 ctttggcgat gagcaggcgc gcgacgtact gcaagttgtc cagcgtttcg ccgcgccggc
     9781 cgatcaagtt gctgagatcg tcgccagtaa cgtcaacttc aatcgccggg ccgcgctgtt
     9841 cgtcttcgac gtcctgtgca acggcttcac gcgccgatac acttacgact aagtgcatct
     9901 tatctaacaa agccgcgacg atgcgttcgg ccacttccgc atcggtgcct gttgaagctg
     9961 cggcttgcag acgatcggat tttaccgtca aacgcacgcg cgtggcgcgt gggtttgttt
    10021 caagcacgag cacatctact tgatcacgcc gaacgttcag cgcctctaaa gctcgggcga
    10081 tggcgttttc agcatcggaa ccaacggctt cgattgattg aggcgtatcc aattcacagc
    10141 ctccagttca tttcttggct ttgggtgcgg ctaaggcttt agtgttggtg gtcgcagact
    10201 tgatcgacgc cttggctgga gcggcggcaa ctgccggtgt catgcgcttg cctagcacca
    10261 tatactgcag cacgccgatg gcgttcgaca cgatgaagta aatgctcaag cccgaggcga
    10321 aattgagcgc gaacaagccg aacatcatgg gcatcatgat attcatctgt ttgctgacgg
    10381 atgccgcgct cggatcgcca ctgggcggag ctgctagttt ctgttgcaag taagtggtga
    10441 caacgactaa agccggcagc aggaacatca agatcggtgt ttgatcggac tgaccgagat
    10501 tcagccacag gaatttgctc tcgatcggta tcaggctggc taattcaggt gcagctcggt
    10561 gggacaacga gagcagctgc aacggattga ctgctaacgc accggtaatc gcttgataga
    10621 atgcgatcat aatgaacatc tgcaaaatca atggcaagca gccaccgaac atgctgacat
    10681 tattctcgcg atacaacttc atctgcgctt cggcgaactt ctgtttgtcg tttccgtatt
    10741 tctttttaag ttcggcgagc tgcggctgca gcgcggccat cttgcgcgac gatcgcatct
    10801 ggggcatggt cagaggcaaa gtaatcaagc gcaccatcac ggtggccacg acgatcgata
    10861 acacaaagct gttgcccagc aggtggtaga aaaacaacag cacgttgacc attcctgaaa
    10921 ctagaaaatc catggtgctt gttcctttct tccgctcgat cggttagggc gccggaggcg
    10981 ggaagacaaa tagctgcgcg ccggtttcgg cattcagacc gtataatcgc gtgggagtgc
    11041 caccagccaa attgggcagg gcgaccaacg ttgtgctata aatcgctggt gtcacgataa
    11101 acatctcgcc gtcttttccg gaaaatggat tttgccaaac tgcacgtccg gtggcggtgt
    11161 ccaacgcgta catcttgaat tgatcggttc cgataaaaac tttaccatcg gcaatcaagg
    11221 gagtggcgcg caccccgccg atcacggtca cggactgcgg ccacttgctc tggccggttg
    11281 cggcatctaa ggcatacaca ttgccgctca gatcgccgaa atataaggtg ccatcagatt
    11341 ccgcaggcgg atcccataag cggccagtgg cggtaaattt ccacttgagt ttgccattga
    11401 tatccagcgc gtacaaattg ctgtcgaaag aacccacgta aattgtatca ctatacacgg
    11461 cgggcgatcc ggcgaatgtt ccaacggtca gcgattgatc gccttgcgcc gggaattccc
    11521 aagcctgatt tccgtccgat tgattaacgg catacacatg atgatcttgc gatgcaatga
    11581 agaccagttt gtcgctgacg gctggcgttg accagatccg agtgttggtc ttcggatcga
    11641 acggttgatt ccaggccggt cgaccagttg ccgcgtccag cgcgaatagc tgcccgaacc
    11701 cgcccgcaaa gaacaatttc tcgttggcta atacgatgcc gccatatatg ttacccggat
    11761 cgatatcggt tttggtcagc ggataagtcc aagtcaccgt cacgttttcg gtgttcatgg
    11821 cgacgaccag cccggaattt ccggtaatag catccgcgcc gaggtataaa gtttttccgt
    11881 cggctgagat agccggttgg ctgtgataac cgatcgaggt ggactgaacc ggcttgatga
    11941 cccagacttg gcgcttggca tcgacattgg ttgccacgtc gagcgcgtaa acttgatcgg
    12001 atgaagcgat ataggccgtg ttgtctcgaa cgaccatgcc gggccaactg gtgactgccg
    12061 gagcgccgcc gcaagcggcc aacacactca tcaacactac catcagagcg atggcgaacc
    12121 atcgatgttt aagcaattga atcaaacaac ctccccatat tatggaacgg gatcgtatcc
    12181 gccggggtga aacggctggc agcggccaat tcgtttaagt gtaagccagc cgccttttag
    12241 tgcgccatat ttttgaattg cctcgtagcc gtagtgcgaa cacgatggct caaatcggca
    12301 ggacggcggt agcactttag cgaaagtcaa ctgccagccc cgaatcaaaa acaaaaaaat
    12361 ggtcttcatc gatcttggtc ttccgcgctc gagtcttcga gccatagctg cgcacgccga
    12421 agtgtggacg tcaaagcctg tgtgatgtcg atgacactgg cctcaagcat aggctgacga
    12481 gcaatgacaa tcaggtcaat cgttgtccgc aagtgtggat atacttgctg caccgccgct
    12541 cggagtttac gtttaactcg attgcggacg actgccccac ctagcttctt gccggtcgcc
    12601 accgataccc tagctcgatc gatcggactt gctgtgcgcg tctgggcaat gatgatcaac
    12661 attggatgtg accagttgcg cccattttgg cggactcgat caaactcgcc gtgtggccgc
    12721 agacgaaata cacgcgccat gctcgccact cagaagcccg gattctcatt gaagccgggt
    12781 ttcttgctca gccggctttc caatcgatgc gcttcgctcg actgggttga taggctaaag
    12841 aatggcggcc tttcaaacgg cgagcttgca atactttgcg gccacccttg gtcttcatgc
    12901 gcgcgcgaaa gccatacgtt cgcgccgtgc gtcgttttct gggttgaaat gtgcgcttgg
    12961 gcattctact ccttcgagat cagtcctatt caaaatgacg tccggtcaaa ctgcaccggg
    13021 cgtggaaatg cgttagttac cagcgagtgg agattataac tgatgtgggg aaactggtca
    13081 aataaataaa atcgcgagac tgacctaatc tcgcgcgtgg gatatcgctt gggatcgatt
    13141 gaagccagat cagccccgat gttccgtatc ccgatagatc ttcgcaccta aagattgaag
    13201 cttcacttcg atctgttcgt aaccccgatc gatctggccg acattgcgga tgatgctctg
    13261 gccctgtgca cacaatgccg ctgagactaa cgccatgcct gcgcggatat cgggactctc
    13321 cagccgttcg ccgtgcagcc aactggggcc ctgcacaata caccgatgcg gatcgcacaa
    13381 gacaatgcgg gcgcccatgc cgatcaattt gtcgacgaag aataatcggc tctcaaacat
    13441 tttctcatga atcagcactg agccggcagc ctgtgtcgcc atcaccagcg cggtgctcat
    13501 catatcggcc gggaaagctg gccacggcgc atcatcaatc ttgggaatgg ctccgccgat
    13561 gtccggcacc accgtcaagg attgttcgcg tcccacgaaa agatcatcac cgcgcgtttc
    13621 gaatttcacg cctaggcgtt cgaacactaa gcgcaccatg ctgaggtgtc gtggcgcgct
    13681 gttgtgaata gtgatttcac cgcccgtgat cgccgctaag ccgatgtacg atccgatctc
    13741 taaataatcg ggaccaatcg taaattcagc gccgtggaga gtggatacag cgtgaatggt
    13801 tagtgtgttt gagccaatgc catcgatctg cgcgcccatt tggttcaaca tgaggcacag
    13861 ttcctgaacg tgtggctcgg aggcggcatt gcgcagaata gttgtgccgt gtgccatgac
    13921 ggcggccatg atcgcattct cagtgccggt gacgctggct tcatctaaga gaatgtcgca
    13981 accgactaag cgatcggcct tcatacgaaa ttcgtgattg atctctatct ctgcgccgag
    14041 tcgagtgagc gccaagaagt gggtgtccac gcgccgccgg ccgatcacat cgccgccggg
    14101 tggtgaaagt tcgatcgatt taaggcgggc taacatcggg ccggccaata aaatcgacgc
    14161 gcgaatctcg cggcacagat cgggatcgag tttggttttg cgcacgttgg cagcccgtag
    14221 atgcagtgta tgcccatcgg gttcggaaat atcgacgccc agatcggcca gcagctgcag
    14281 catcgtgcgc acatcgcgaa tgcgaggcac gttgtgcaag atgatgggct gatcggtaag
    14341 catgcaggcg gccatcaagg gcagggcggc atttttgttg ccgctaggag ttgtttggcc
    14401 ggtgagcggc tgtccgcctt cgatcacaaa ttggtccata gtttttccaa gtcttttgaa
    14461 ttctacctta agccgatggg cttcgcaaac cgatcgagca atgattgcgc catgattgct
    14521 ctacgattga catcgttttc cccccgtctc ggctaagaaa accgctgttt tgcccggaaa
    14581 atcggcaaaa aatacctatt gtttacttcc aactttgcct gacgtgtggt aaaattagat
    14641 gtcgtggata gaagacccgt tcgtcgcccc atattgagcg atcgcccaac tgtaggtgtg
    14701 attgagtcga tttccaccgg atttgacgca gtggcgcgcc atccgtggtt gttgattgtc
    14761 cctttcgcgc tcgatttatt tttgtggctg gggccgcgca ttcaggcgag tgcgctatat
    14821 gatcaatttg tgccgtatct ccaaaacttt ccggttgcat tggatgttga tacacgcttg
    14881 gctgtgcagc agtttatgca ggcgttgcag gaattctttt caaagtttaa tttattctcc
    14941 tggctaagtt cgggcttgtt tggcgtgccg acattgaacg cgggcgggga ttcaacggct
    15001 aagttggtga cgggcggagt gccggcggtc tggcagatca gtgatgtggg cagctacatc
    15061 ttgctcatca ttgcgttgaa tattgtaggc ttgctcgccg ccgcgctgtt gtgggtcatg
    15121 ctgatcgacg ttatgcggaa ggaagtcttg aacctccgca atatgttgaa gcgatcgttc
    15181 agcctgtggg tccgactgct gacttttgtc ttggctttac tggcagtatt catcgtctta
    15241 gcgcttggtc tgtcagttgt tactgcgctg atcggcgcga ttagtttatc gttcgccgca
    15301 ttcattccgc tggtggtgct atcgctggga gcgtggggct tattttatct ggcgtttaca
    15361 ttacacggcc tggccttata caatttgcca gccgtgcgat cgatgcgctt gagcatgctg
    15421 atgactcgct tgttttttgc gccgactttg ggtctggtgg tgatctcgat cgcgatttat
    15481 atgggcatcg gcttgatttg gaccaacttc gcgattgatt cgtgggcaag gttgatcgcc
    15541 atgctgggca atgcttttat cgtagccggt ttgatgatgt cctctttgat ctattaccaa
    15601 aatcgatcga gcatcgtgct tgatttcttg cagtctgtta agccaaccga gaattaatta
    15661 ttagaaattt gatgttgggg ttggaagttg aattttccat tcgccaactt ctatcgttta
    15721 aggagtgata gctgtggcag aacctaaagc caaatatgat gcgagtcaga ttcaggtact
    15781 ggaaggtcta gaggcagttc gccgccgacc gggtatgtac atcggcagta cggacatccg
    15841 cgggctgcat cacttaattt atgaagtagt cgacaatgcg atcgatgaag cattagccgg
    15901 tgaagccgat aacattgatg tgaatattca cgcggacatg agtgtatccg tgcgcgataa
    15961 tggccgtggc attccggtcg gcccgcaccc aacgcagaag gatgcgaacg gcaaggcgat
    16021 ggatgccgtc gaagtcgtca tgacgatttt gcacgcaggc ggcaagttcg gcggctcagg
    16081 ttataaggtc gcgtcaggct tgcacggcgt gggtgtcagc gcggtgaatg ccttaagtga
    16141 atggctgatc acaacggtgc atcaagacgg taaagaatat cggcaagagt ataaacgcgg
    16201 caaaccgacc gggccagtca agctaatcgg gaaactggct aagaccgaaa catgggtacg
    16261 cggcaccgat caacgcttca tgccggatcg atcgatcttc aaagacgaga ttgattacaa
    16321 attcgatacc ttgattcaac gcttccgtga aatggcgttc ttgacacgca acttgacgat
    16381 caagtttatc gacgaacgcg atggacatga gatgtcattc catttcgaag gtggcgttcg
    16441 atcgttcgtg cgttatctca acaagagccg cgcagtgctg catccgccgg tgtatgtcga
    16501 taagcagata aatggtacgg cggttgaagc agcgatccaa tacaccgatg gttacactga
    16561 atcggtctac gcatttgcca acaacatcaa cacgatcgat ggcggcacgc acgtcaccgg
    16621 ttttcgatcg gcgctgacgc gcacgatcaa cgattacgcg cgcaaagccg gactgctgaa
    16681 agattccgat ccgaatttct ccggcgatga tgtgcgcgaa ggcttgacgg caatcatcag
    16741 catcaagctg caagacccgc agtttgaatc gcagaccaaa tcaaagttag gtaacgccga
    16801 agtgaagggc caggttgaaa ctgcggtcgg cgaagcgttt acagaatttt tggaagagaa
    16861 cccgcgcgaa ggaaaagcga tcatcacgaa gtgcttgacc tcgtcgcgcg cgcgtgaggc
    16921 cgcacgtcag gcgcgcgatc tggtgattcg taaatcggcg ctggagtcga tgacgctgcc
    16981 cggcaaattg gccgattgct cggagcgtga tcccgctaaa tgtgaaattt acattgtcga
    17041 aggcgacagt gccggtggct cggccaagca gggacgcgat cggcacttcc aggcgatctt
    17101 gccgttgcgc ggcaagattc tcaacaccga gcgcgcgcgc atggataaga ttctggccaa
    17161 caatgaagtg aagtcgttga tcagcgcact gggctgcggc attggcgagc aattcaactt
    17221 ggataatctg cgctaccacc gcacgatcat catgaccgac gccgacgtcg acggcagtca
    17281 catccgtaca ttattgctca cctttttctt tcgctatatg caaagcttgg ttgaaggcgg
    17341 ttttctttac attgcgcagc cgccattgta tcgcatcgag tcgaagaagc tcggcgtgaa
    17401 atatgtctat aacgatgccg acaaagatat cgaagtggcg ggactcaagg acgagaaagt
    17461 ctcgattcaa cgctataagg gtctgggcga aatgaacccg gagcagttgt gggaaacaac
    17521 tctcaatcct gagaatcgaa ccttgttgca agtcaccgtc gaagacgccg ccgccgccga
    17581 tcgaacgttc gacatgctca tgggtgatga agttccgccg cgcaaacgct tcatccagac
    17641 gcacgcgaaa gaagttcgca atctcgatat ttaatcgaga caaatttgat aggtgttcaa
    17701 ctggttgtta ggccaaattg atctgcgagt agagggcgga gggcaattga ggactctgcc
    17761 ttcttt
//
LOCUS       DASRTJ010000102         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_308584,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000102 DASRTJ010000000
VERSION     DASRTJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308584"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..971
                     /gene="secF"
                     /locus_tag="VFF70_02135"
     CDS             51..971
                     /gene="secF"
                     /locus_tag="VFF70_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZY43522.1"
                     /translation="MWDIVGKRYRWFALSLLVIVPGLIFLFAFGLPFAIDFTGGSLLE
                     LQFKNTAPATADVNAVYAQFGFPDSQVQSTENNGVQIRSKSMEDSTKNSIEAALREKF
                     GDFTELEFGSVSAAISAEVVQGGIAAVGFAAVGILAYLWYAFRKVPHSFRYGVAAIIA
                     MLHDVAVMVGAAAIFGKLLGWEIDALFLTALLTVIGFSVHDTIVVFDRIRENLIRRRG
                     ETYDRIVNHSVIQTLDRSISTQLTVFFTLLALALFGGVTIRHFIVVLLVGILSGTYSS
                     IFNAAQILVVWENKEWRTWFRRKPAEPATM"
     gene            1230..2195
                     /locus_tag="VFF70_02140"
     CDS             1230..2195
                     /locus_tag="VFF70_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZY43523.1"
                     /translation="MRALYVDHGLRFEKDYSIPTPPPGEALVRVLQAGICNTDLELVK
                     GYLNFKGVPGHEFVGMVEKAAKREDLIGQRVAGEINAACGKCETCRAGRPTHCPNRTT
                     LGINRRDGAFADYLLLPFENLHPLPDPVSNDQAVFVEPLAAACEILEQIKIHPTDRVV
                     VIGDGKLGLLCAQVIALTGCWLSVLGRHSDKLQILNERHIHTTIDINEFAPASIDIVV
                     EATGTPSGFETARKIVRPRGTIVLKSTYNSDLNVSLSLVVVDEVSLIGSRCGPFEPAI
                     RLLANRQVEVDRLIQARYSLNEGLQAFERAATKGTMKVIVEMTNE"
BASE COUNT          519 a          550 c          623 g          537 t
ORIGIN      
        1 cccttgtgtt aatggggaag ggttgggagg gggttcagga gaacagtgct atgtgggata
       61 ttgtaggaaa acgttatcgg tggtttgccc tatcgctact cgtgatcgta ccaggcctga
      121 ttttcttgtt cgcattcggt ctgcctttcg cgatcgattt taccggcggt tcattgttag
      181 aactgcagtt taagaatacc gcgcctgcta cggccgacgt caacgcggtc tatgctcaat
      241 tcggttttcc agacagtcag gtgcagagca cggaaaacaa cggcgtgcag attcgatcga
      301 agtcgatgga agattcaaca aagaatagca ttgaagccgc gctgcgagaa aagttcggcg
      361 acttcaccga attagaattc ggatcagtca gcgcggccat cagcgccgaa gtggtgcaag
      421 gcggcatcgc cgccgttggt ttcgccgcag tcggcattct cgcttatctc tggtatgcct
      481 tccgcaaagt gccgcattca tttcgatatg gtgtggcggc gatcatcgcc atgctacacg
      541 atgtggccgt catggtcggc gcggcggcga tcttcggcaa actgctcggc tgggagatcg
      601 atgcgctctt tttgaccgcg ctgctcaccg tcatcggctt ctcagtgcac gatacgatcg
      661 tcgtgttcga tcgcatccgc gagaacttga tccgacgccg cggcgaaacg tacgatcgca
      721 tcgtaaatca tagtgtgatc cagacactcg atcgatcaat cagcacccaa ctgacggtct
      781 tcttcacttt gctggccttg gccttgttcg gcggcgtcac cattcgacac ttcatcgtcg
      841 tgctgctagt cggcatcttg agcggcacgt actcatcgat cttcaacgcg gcgcaaattc
      901 tagtcgtgtg ggaaaataaa gagtggcgca cgtggttccg tcgcaagccg gctgaaccag
      961 ccacaatgta gtataaagtt gtgataacat agagccagtg gagaaatctg ctggctcttt
     1021 ttatttttgg agcgccgact ttagtcggct tattgcaccc gaggatgtgc cgactaaccc
     1081 taaagggcgt ttcgcagagt cggcgttccc aatccaatat gctatacaag atcgaacccg
     1141 ccgaggtttg ataacacaaa ggtccgaaga ctcaaaggaa aactaaaata acttagcgcc
     1201 ttggggtctt ggtggttaag caggtaacta tgcgagcttt gtacgtcgat cacggactac
     1261 gctttgaaaa agattattca atcccaacac cgccgcccgg cgaagcattg gtgcgcgtct
     1321 tgcaagccgg aatctgcaac accgatcttg aactcgttaa aggctacttg aatttcaaag
     1381 gcgtgccggg gcatgagttc gtcgggatgg ttgagaaggc cgctaagcgc gaagatttga
     1441 ttgggcaaag agtggccgga gaaatcaatg cggcttgcgg aaaatgtgag acttgccggg
     1501 ccggtcgacc gacacactgc cccaatcgta ccactctcgg catcaatcgg cgtgatggcg
     1561 cgttcgccga ttatctactc ctgcctttcg agaatctcca tcctctgccc gatcctgtct
     1621 cgaatgatca agccgtgttt gtcgaaccac tcgctgccgc gtgcgaaatc ttggagcaaa
     1681 tcaaaatcca tccgacggat cgagtggttg tcattgggga tggcaagttg ggattgttgt
     1741 gcgcgcaagt gatcgcgctg acaggctgtt ggctatcggt gctcggccgg cattcagata
     1801 agttgcaaat tctgaatgag cggcacattc atacaacgat cgatatcaac gagtttgctc
     1861 ctgcttcgat cgatattgtc gtcgaagcga cgggtacgcc gagcggcttt gaaacggcgc
     1921 ggaagatcgt tcggcccaga ggaacaatcg tgttaaagtc aacctacaac agcgatctca
     1981 acgtgagctt gtcgctggtc gtcgtcgatg aagtatcgct gatcggatcg cgctgcgggc
     2041 cgttcgaacc ggcgatacgc ttgttagcga atcggcaagt ggaagtcgat cgattgattc
     2101 aggcgcggta ctcgctcaat gaggggcttc aagcgttcga gcgtgcggcg acaaaaggaa
     2161 cgatgaaagt aattgttgaa atgacaaatg aataatgacc aatgataaat tatgcgagga
     2221 tatattgat
//
LOCUS       DASRTJ010000103         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_116077,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000103 DASRTJ010000000
VERSION     DASRTJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116077"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..2011)
                     /locus_tag="VFF70_02145"
     CDS             complement(221..2011)
                     /locus_tag="VFF70_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY43524.1"
                     /translation="MTPTAVTSSAPSPFAVFRNRSFSLMWTGQLISTIGSALTSLAAS
                     IYVYHVTNSALSVGLMLMATAAPTILVGLIAGVFVDRYDRKKIMIAADLIRAVLVFLL
                     PILLPVSIVWLYVIVALSSAVGQFFEPAYESLLPEIATDAELAAANSFIAISSFGATA
                     IGFAASGLIGSQNVNIAFWVDAVSFLLSAFCILLIKFPKLAAQEATTVKVVLRNLKAG
                     AKFLINSPMLRSLFIVSIPLFVSFGLANSLLLPFAKNALHATESQYGLQEGLTSIGFV
                     VGSLLMARLTERLREGQWIAISIIGMALAGIGYSLMVSIPIAIGIQMASGLLNAPSSV
                     GRRLIVQRNTPREMRGRVNSAFAVSRDVMFLFGMAAAGLADVIDVRIMYLIASLILLG
                     SGALSLIARGLGQPAAEWRRAMTLLRTAPSMSGLGLGRAALLADMDKLVGLLPSLSTL
                     SAKDRSDFVAHSIVNEAPAGTTIIRVGEQGDTAFFILSGRVVAGLPTPDGGYRGLSAL
                     NAGDFFGEIAALTGAARTANIVADEPTTVLQVPAKTMRALMTNAQLSQLFLSKITERL
                     SRTSVSDLPRFAGYDQQSLRDLRTAPAAES"
     gene            complement(2124..3884)
                     /locus_tag="VFF70_02150"
     CDS             complement(2124..3884)
                     /locus_tag="VFF70_02150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZY43525.1"
                     /translation="MNEYKQSRWSLKDILDEQQIDQQLEQLEQAVAAFEALRPQLTPD
                     ISESEFLDVLRRYEELSATLRALGAYAELWFSEDTQNQAALSLQGRLDQIGADASNRL
                     VFFSLWVKDLPDESIDRLMQLSGDRRYFIDSLRRFKPYLLSESEEQLITLKDVNGIEG
                     LLTLYEMITNKFEFNLHIEGDTKTLTRDELQNYFRNPSPEVRAAAYQELYRVFAENSA
                     VLAQIYTYRARDWHSEMIDLRHFAEPISMQNLANDIPDRVIDTILNVCRQNSPLFQRY
                     FKLKAKWLNVDKLRRYDIYAPLAEAEKTYTYQQATDLVLSTFNNFSPKVSERAQRVFD
                     QGHLDAEVRPGKRGGAFCYAVLPTLTPWVLLNFNGRPRDVAVMAHELGHAVHAMLAAD
                     HSILTFHSALPLAETASVFSEMLMTDRLLHDENDKAVRRDLLSSFIDDAYATVLRQAY
                     ISIFEREAHRLIVENKSIDEVCALCLSIVREQFGDAVEVSDEFKWEWITIPHSYGTPF
                     YTYAYSFGQLLVLALYQQFRNEGEPFKPRYLKILASGGSASPAKILNVAGIDITSAKF
                     WQGGFDVIKGFMTELEQLSK"
     gene            complement(3976..>4211)
                     /locus_tag="VFF70_02155"
     CDS             complement(3976..>4211)
                     /locus_tag="VFF70_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43526.1"
                     /translation="QFIFADPTVQRIGHALLPQFDPSLKFAEALGFERAGGPFKNKRG
                     FGLYSFVKKRPDIPPTPGAQIWQEWKAKINGAN"
BASE COUNT         1003 a         1209 c         1122 g          877 t
ORIGIN      
        1 agctgcgccg atcggtttac gccgctcgac cggtttcttc ttgatcgacg gtggtgtttt
       61 tcgcggagca gatcgcttag tagttttcat ctgtacgtac tccaatgatt taagatgatc
      121 aggcgcaggc cgcctatcat catttcagca caagcgtgtg gtggcgtctg tcgctggcac
      181 gacagagatc ggggcaatca accggccgcg agttacagct tcatgactca gccgccgggg
      241 cggtgcgcag atcacgcagt gattgttgat catagccggc aaaacgcggc aaatctgaca
      301 cgctggtgcg gctcagccgt tcggtaatct tggacaggaa cagttggctg agctgcgcgt
      361 tagtcatcag cgcgcgcatt gttttagctg gaacttgcag gaccgtcgtc ggttcatcgg
      421 ctacgatgtt ggccgtgcgc gctgcgccgg tgagtgccgc aatctcgccg aagaaatcac
      481 cggcgttcaa cgcggataag ccgcgataac caccatccgg tgtgggcagt ccggcgacca
      541 cccggcccga caaaatgaag aaggcggtat cgccctgttc gcctacccga atgatcgtcg
      601 tgccggctgg cgcttcattg acaatggaat gcgcgacaaa atccgatcga tctttcgcgc
      661 tcaacgtgga gagggatggc aataagccga ccaatttgtc catgtccgcc aacagcgcgg
      721 cgcggccaag acccagaccg gacatcgagg gcgcggtgcg caacaaggtc atggcgcgtc
      781 gccattcggc cgccggctga cccaggccgc gcgcgatcaa actcaacgcg cccgagccga
      841 gtaaaatgag cgaagcgatc aaatacatga tgcgcacatc aatcacatcc gctaacccgg
      901 cggccgccat gccgaacaag aacatcacat cgcgcgaaac ggcgaaggcg ctgttcacac
      961 ggccgcgcat ctcgcgcggc gtattgcgct ggacgatcaa acgccggccc acggaagacg
     1021 gcgcattcaa caagcccgac gccatttgaa tgccgatcgc gattggaatg gagaccatga
     1081 gcgaatagcc gatgccggcc agtgccatac cgatgatgct gatggcgatc cattggcctt
     1141 cacgcaagcg ttcggtcaag cgggccatca acaggctgcc gaccacaaag ccgatcgagg
     1201 tgagaccctc ctgcagtcca tactgcgatt cggtggcgtg cagcgcgttc ttcgcaaacg
     1261 gcagcagcag tgagttcgcc agtccgaagc tgacaaaaag cgggatcgat acgatgaata
     1321 gcgaccgcag catcggcgag ttgatcaaga atttagcgcc cgccttcaag ttgcgcagca
     1381 ccactttcac ggtcgtcgct tcttgcgcgg ccaacttcgg gaacttgatc agcaagatac
     1441 aaaaagccga gagtagaaaa gataccgcat ccacccagaa cgcgatattc acgttctgcg
     1501 atccgatcaa accggaagcc gcaaaaccaa tcgccgttgc gccgaaggag ctgatcgcga
     1561 tgaaagaatt ggcggcggcc agttcagcat ccgtggcgat ttccggcagc aggctctcgt
     1621 acgccggttc gaagaactgc ccgaccgcac tcgacagcgc gacgatgaca tacagccaca
     1681 caatactcac gggcagcaga atgggcagca aaaaaaccag cacggcacgg atcagatcgg
     1741 cggcgatcat gatcttcttg cgatcgtagc gatcgacaaa cacgccggcg attaaaccga
     1801 ccaggatcgt cggcgcggcc gtagccatca acatcaagcc cacactcaac gcggagttgg
     1861 tgacatgata tacatagatc gatgcggcca atgaggtcag cgcgctgccg atcgtggaga
     1921 tcaattgacc ggtccacatc aaagagaaac tgcgattgcg gaagactgca aagggtgatg
     1981 gtgcagaaga ggttacggca gttggtgtca ttcaattccc ctttggcttg atagattcat
     2041 ttatccataa tggacctgga gagcgaagac ctgcttgaat tctgcgtgta tctgagtctc
     2101 cttcatggat cgtttcttct aaactattta ctcaattgct caagctccgt catgaaaccc
     2161 ttaatcacat cgaagccgcc ctgccagaac ttggccgaag taatatcgat gcctgctaca
     2221 ttcaaaatct tcgccggtga agccgatccg ccgctggcta agatcttcaa atagcgcggc
     2281 ttaaacggct cgccttcatt tcggaactgc tgatacagcg ccagcaccaa caattgaccg
     2341 aagctgtagg cgtaggtata gaacggcgtc ccgtaactgt gtggaattgt gatccattcc
     2401 cacttgaatt catcgctgac ttcgactgca tcaccgaact gttcgcgcac aatcgacaaa
     2461 cacagcgcgc acacctcatc gatcgatttg ttctccacga tcagccggtg cgcctcgcgc
     2521 tcgaaaatgc tgatgtaggc ctggcgcagc acggtggcat acgcatcgtc gatgaaactg
     2581 gacagcaaat cccggcgtac ggccttatcg ttttcgtcgt gcaataaccg atcggtcatc
     2641 agcatctcgg agaacaccga agccgtctcg gccagcggca gcgccgaatg gaaagtcaag
     2701 atcgaatgat cggcagctaa cattgcgtgc acggcgtgcc ccaactcgtg cgccatcacc
     2761 gccacatcgc gcggtcggcc gttgaaattc aacagcaccc acggcgtcag cgtcggcagg
     2821 acggcgtagc agaatgcgcc gccgcgtttg ccgggccgca cttccgcatc cagatgacct
     2881 tgatcgaaca cgcgctgggc ccgctcgctg accttcggcg aaaaattatt gaacgtgctc
     2941 aacaccaaat cggttgcctg ttgataggta taagtctttt cggcttcagc cagtggcgcg
     3001 taaatatcgt accggcgcaa tttatcgacg ttcagccatt tggccttcaa cttgaaatag
     3061 cgctgaaaga gcggggaatt ctggcggcag acgttcaaga tcgtatcgat cacgcgatcg
     3121 ggaatatcat tggccaaatt ctgcatcgag attggctcag cgaagtgccg cagatcgatc
     3181 atctcgctgt gccaatcgcg tgcacgatag gtgtagattt gtgccagcac cgcgctgttt
     3241 tccgcgaaca cgcgatacaa ctcttgataa gccgcggcgc gcacctccgg cgacggattg
     3301 cggaagtaat tctgcaattc atcgcgcgtc agcgtcttgg tgtcgccctc gatgtgcagg
     3361 ttgaattcaa atttattcgt gatcatctca tacaacgtca acagcccttc gatgccgttg
     3421 acatctttca aagtgatcag ctgctcttcc gattcgctga ggagatacgg cttaaagcgc
     3481 cgcagtgaat cgataaagta acgccgatcg ccgctgagct gcatcaaccg atcgatcgac
     3541 tcatccggca agtcctttac ccatagcgag aagaacacca ggcggttgct ggcatccgct
     3601 ccgatttgat ccaaacggcc ttgcaaactc agcgcggctt gattctgggt atcttccgag
     3661 aaccacaact cggcatacgc gcccagggcg cgcagcgtcg ccgacaattc ttcataacgg
     3721 cgcagcacat ctaaaaattc ggattcggaa atgtccgggg ttaattgggg acggagggct
     3781 tcaaaagctg caacagcttg ttccaactgc tcaagctgct gatcgatctg ctgctcatcg
     3841 aggatgtctt tcaaactcca gcggctttgc ttgtattcgt tcaactcatt cctccccagc
     3901 ttggctgaat cgaagtctaa tgccagaccg ttaaatcgtc aactgcattg agatcatcat
     3961 ccacacacga tcgtcttaat tcgcgccgtt gattttggct ttccattcct gccagatttg
     4021 cgcgcccggt gtcggcggaa tatcgggccg cttcttcacg aatgaataca aaccaaaacc
     4081 gcgtttattt ttgaatggcc cgcccgcacg ttcaaagccc agcgcttcgg cgaacttcaa
     4141 tgatggatcg aattgcggca acagcgcatg accgatgcgc tgcacagttg gatcagcgaa
     4201 aataaattgc t
//
LOCUS       DASRTJ010000104         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_43963,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000104 DASRTJ010000000
VERSION     DASRTJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43963"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFF70_02160"
     CDS             <1..247
                     /locus_tag="VFF70_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HZY43527.1"
                     /translation="FSRYLYTTDLPDPDLIVRTSGEMRVSNFLIWQGAYAEYYATEAL
                     WPDFNEAELQKALDHYTRRERRFGLTTEQMAATQSAK"
     gene            411..1211
                     /locus_tag="VFF70_02165"
     CDS             411..1211
                     /locus_tag="VFF70_02165"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZY43528.1"
                     /translation="MFLTRILSALILAPIVVGLIWIGGWPFSVLLVAFLSLAAIEFAQ
                     VMRRGGFEPPIFFTLILIIISTADAFFPDVQLLRPAIAFVLISSLTWQLFNRTGTPTI
                     NWALALIGGLYLGIAGAHFILLRQLSSGERWLLLSLTGTWLADSGAYLVGSRIGRHKM
                     TPTLSPKKSWEGLIGGIVISVLINMIVAALLDLPIIHGAALGLIGASIGLLGDLSISM
                     IKRQVGVKDTGQLIPGHGGALDRLDSVLFTVIVSYYYIIWIALVPALP"
     gene            1224..2225
                     /gene="glpX"
                     /locus_tag="VFF70_02170"
     CDS             1224..2225
                     /gene="glpX"
                     /locus_tag="VFF70_02170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZY43529.1"
                     /translation="MVNSTKILTDPDRNLGLELVRVTEAAALAAARWMGRGDKINADQ
                     AAVNAMRYLFNTVSMDGIVVIGEGEKDEAPMLYNGEHLGTGEPPQVDIAVDPIDGTRL
                     TSLGMPGAVAVVAMAERGTMYNPKHLVYANKIAVGPRAKGVIDINAPVKDNLAKVARA
                     LDKDVNDLTVVILDRDRHKELIKQVRACGARIKLISDGDIAGGLVTAFEDAGQDILMG
                     IGGSPEAVITAAALKCLGGEIQCKLWARDEKERIKALDLEIDLDQVLTTNDLVAGENV
                     FFAVTGITDGELVGGVRYDGRGAHTTSLVMRSLSGTIRKIESDHHLDKLKLLTNLYE"
     gene            2288..3565
                     /gene="rho"
                     /locus_tag="VFF70_02175"
     CDS             2288..3565
                     /gene="rho"
                     /locus_tag="VFF70_02175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZY43530.1"
                     /translation="MDFVELDNKTLSELRELAKSMEITGYSRLKKDDLIIALLRAKAE
                     GEGYIFGGGVLDVVDENIGFLRSERYLPGPDDVYVSQSQIRRFGLRTGDMVIGQVRPP
                     KDSEKYFGLLRVEAVNGIDPEQAKLRPNFEKLTPIFPNKRFNLETDPANLTTRLLDLV
                     APIGFGQRGLIVSPPKAGKTTVLKHIANAISTRYPDVHLMVALIGERPEEVTDMDRSV
                     DAEVISSTFDEPPQSHIRVAEMALARAKRLVESGRNVAILMDSITRLSRAYNLVVPSS
                     GRTLSGGLDPAALYPPKGFFGAARNIEEGGSLTIIATCLVDTGSRVDDIVYEEFKGTG
                     NMELHLSRRLQERRVFPAFDMERSSTRREELLLEPDTLSKVWLMRRMLSQMMAPPPSG
                     AGMDIANAQEALNGRMSRSKTNVEFFASLRDDF"
     gene            3718..5025
                     /locus_tag="VFF70_02180"
     CDS             3718..5025
                     /locus_tag="VFF70_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HZY43531.1"
                     /translation="MSEIELVPRRAARRLTQPLVEANLVKSIEARRKNTPTLEASARV
                     TTSVVSVSNTDWVELTPSSFGSLFARLFARTAVQFSGTAWLATGDRAKATMLRFISHI
                     VILAVVLGAAVIAGSTHTPRVLAGNTINSVQRPAAADETFFGEGGPIVIDQLSVSPMA
                     NPFTIVPNRPRKDIVVYMVQSNDTLSQIAYLYGLKPETVLWANTDILHDDPHYLTPGM
                     ELKIPPVDGIIYTVKAGDTVDDIAKKFKTSADAIYTDGVLWNQLKPGQLPVVGSVLIV
                     PGGSRDFQIVNLVAQTFFSSQSSSGYCKNVAPGLIGTGTFIWPIQQHWVSGNNYAPWH
                     PGIDLAAHTGDQVYAVDNGIVIYAGWNNSGYGNLVVIDHGNGWISYYAHNSIILVTCG
                     QNVAQGTVISAAGSTGRSTGPHLHIETRFNDVPQNPFNVLPPP"
BASE COUNT         1189 a         1484 c         1411 g         1217 t
ORIGIN      
        1 cttcagccgc tatctgtata ccaccgacct gcccgatccc gatctcatcg tgcgcaccag
       61 cggcgagatg cgcgtcagca attttttgat ctggcagggc gcgtacgccg aatactatgc
      121 gaccgaagcc ttgtggcccg atttcaatga agccgaattg caaaaggcgc tcgatcatta
      181 cacgcggcgc gaacggcgct tcggcctgac gaccgagcaa atggcggcca cgcaatctgc
      241 gaaataacta taacccgatc ggttgagctg gcccgatcga gaaaaacctt gcgaagagcc
      301 tgccctgagc gcagtgaagg gttggactcg aagattttcg gtcaacgctg aacccttcgc
      361 aagtttgtcc caagtcaccg atacaacatc ttgagttcat ttcaacccac atgttcttga
      421 cgcgtatcct gagtgcgctg atcttagcgc cgattgtggt cggcttgatt tggatcggcg
      481 gctggccgtt tagcgtctta ctggtcgcct ttctcagttt agccgcaatc gaatttgcgc
      541 aagtgatgcg gcgcggcgga tttgaaccgc caatcttctt cacgctaatc ctgatcatta
      601 tcagcaccgc cgatgcgttc ttcccagatg tccagctctt gcgaccggcc atcgcgttcg
      661 tgctgatcag ctcattaact tggcagctct tcaatcgcac cggcacccca acaataaatt
      721 gggcgttagc cttgatcggt ggtttgtatt taggcatcgc gggcgcgcac tttattttat
      781 tgagacaact gtctagcggt gaacgctggt tgttgctcag cctgaccggt acgtggttag
      841 ccgacagcgg cgcttattta gttggctcac gcatcggtcg gcacaaaatg acgccgacgt
      901 taagcccgaa gaaaagttgg gaaggcttga tcggcggcat cgtgatcagc gtgctgatca
      961 atatgatcgt tgccgctctg ttagatttgc cgatcattca cggcgcggcc ttagggttga
     1021 tcggcgcatc gatcgggttg ctgggcgatc tatcgatttc aatgatcaag cggcaggtcg
     1081 gcgttaagga caccggtcag ttgattccgg gccacggcgg cgcgctcgat cgattagaca
     1141 gtgtgttgtt taccgtcatc gtcagttact actacatcat ttggatcgcc ttagtcccag
     1201 cactcccttg aggaaaagaa ctcatggtta attccactaa aatcttaact gatcccgatc
     1261 gcaatctcgg cctcgaactg gtgcgcgtca ccgaagccgc cgcgctggcc gccgcgcgct
     1321 ggatggggcg cggcgacaag atcaatgccg atcaagccgc ggtcaatgcc atgcgctatt
     1381 tgttcaacac cgtcagcatg gatggcatcg tcgtgatcgg cgaaggcgaa aaagatgaag
     1441 cgccgatgct gtacaacggc gaacatctgg gcacgggcga gccgccgcag gtggatattg
     1501 ccgttgaccc gatcgatggt acgcgcttaa cgtctttggg gatgcccggc gctgtggccg
     1561 tagtcgcgat ggcggaacgc ggcacgatgt ataaccccaa gcatctagtg tatgcgaata
     1621 agattgccgt cgggccgcga gccaaaggcg tgatcgatat caatgcgccc gtcaaagata
     1681 atttagcgaa ggtcgcccgc gcgctcgaca aagatgttaa cgatctcacc gtcgtcattc
     1741 tcgatcgaga tcgacacaag gaattgatca agcaggttcg ggcttgcggc gcgcgcatca
     1801 aactcatcag cgatggcgac atcgccggcg gattggtcac cgcgtttgaa gacgccgggc
     1861 aagacatcct catgggcatc ggcggatcgc ccgaggccgt catcaccgcc gcggccttga
     1921 aatgcctggg cggcgaaatt caatgcaagc tatgggcgcg cgacgaaaag gaacgcatca
     1981 aagcgctcga tctggagatc gatcttgacc aagtcttaac caccaatgat ctcgtggccg
     2041 gcgaaaatgt tttctttgcc gtgaccggca tcaccgacgg tgaactggtc ggcggcgtgc
     2101 gctatgacgg tcggggcgcg cataccacct cactggtcat gcgcagtctc agcggcacca
     2161 tccgcaagat cgaatctgat catcatctcg ataaactcaa gctgctcacc aatttatatg
     2221 aataagacat caagcctccg gttattgatc gcctactctt gtgccggact gatggaggaa
     2281 taaactcatg gatttcgtag aactcgacaa caaaactctc agtgaactgc gtgaacttgc
     2341 caaatcgatg gaaataaccg gctacagccg cctcaagaaa gatgatctca tcatcgcctt
     2401 gctgcgcgcc aaagccgaag gcgagggcta catctttggc ggtggcgtgc tggacgtggt
     2461 cgatgagaac atcggctttc tgcgcagcga gcgctatcta cccggcccgg atgatgtgta
     2521 cgtcagccag agccagattc ggcgcttcgg tctgcgcaca ggcgatatgg tcatcggcca
     2581 agttcgcccg cctaaagaca gcgagaaata tttcggactg ctgcgcgtcg aagcggtgaa
     2641 cgggatcgat ccggaacaag ccaagctgcg cccaaatttt gaaaagctca cgccgatttt
     2701 tccgaataag cgctttaatc tcgaaaccga tccggctaat ctgaccacgc gtttgctcga
     2761 tctcgtcgcc ccgatcggct ttgggcagcg cggcttgatc gtgtcgccgc caaaagccgg
     2821 caagacaacg gtgctcaagc acatcgccaa tgcgatctcg acacgctatc ctgatgtgca
     2881 tttgatggtc gcgctgatcg gtgagcggcc tgaagaagtg acggatatgg atcgatcggt
     2941 cgatgccgaa gtgatcagca gcacgttcga tgaaccgccg caaagtcaca ttcgcgtggc
     3001 cgagatggcg ctggctcgcg ccaaacgctt ggtggagtcc gggcgcaatg tcgcaatctt
     3061 gatggactct attacccgct taagtcgcgc ctacaacttg gtcgtcccgt cgagcggcag
     3121 aactctatcg ggcggcctgg atcccgccgc cttgtatcca cccaaaggtt tctttggcgc
     3181 agcccgtaat atcgaagaag gtggcagcct gaccatcatc gctacttgct tggttgacac
     3241 cggcagccgc gtcgatgata ttgtttacga agaattcaaa ggcaccggca acatggaact
     3301 gcatctcagc cgccgcttgc aagagcgccg cgtcttcccg gcttttgata tggaacgctc
     3361 cagcactcgc cgcgaagagt tgctccttga gccggatacg ttgagcaagg tttggctgat
     3421 gcgccgcatg ctgtctcaaa tgatggctcc accaccatcc ggcgcaggta tggatattgc
     3481 caacgcccaa gaagctttga acggtcgcat gtctcggtca aagacgaatg tcgaattctt
     3541 cgccagcctc cgcgacgact tctaaaattg ttaaagaaac aatcagttca cataagataa
     3601 atcggggcgt tttcttgcgc agatcgatcg acatggtata atcgcgcccg taggagttgg
     3661 gccttcgtag atactaagtt gccgcttccg attcgccttg cttttgtttt tcgatttatg
     3721 agcgaaattg aattagtccc acgccgcgca gcccgccgac tcactcagcc tttggttgag
     3781 gcgaatttgg ttaaatcgat cgaggcgcgg cgcaaaaata ccccaacgct tgaagcctct
     3841 gctcgagtta caacttctgt cgtgtctgtc tctaacaccg attgggttga gctgactcca
     3901 tcgagttttg gttccctctt tgccaggttg ttcgctcgaa ccgccgtcca atttagtggg
     3961 accgcttggc tagccacggg cgatcgggct aaagcaacta tgctgcgctt tatttcgcac
     4021 atcgtcattt tggccgtcgt attgggcgcg gcggtcattg ccggcagcac acatactccg
     4081 cgcgtgttgg ccgggaatac aatcaacagt gttcagcggc cggctgccgc cgatgaaact
     4141 ttctttggtg aaggcgggcc gattgtcatc gatcaactta gtgtttcgcc gatggcgaac
     4201 cccttcacta tcgtgccgaa tcgaccgcgc aaagatattg tagtctacat ggtccaatcc
     4261 aacgatacct tatcgcaaat tgcttatttg tacggcctca aaccagaaac cgtcttgtgg
     4321 gccaacacag atatattgca cgatgaccca cattatctaa cgccgggtat ggaactaaaa
     4381 ataccgcccg tggatggaat catttacacg gtaaaagccg gtgatacggt tgacgatatt
     4441 gccaagaaat tcaaaacaag tgccgatgcc atttacactg acggcgtcct atggaatcaa
     4501 ctcaagcccg gtcagctgcc cgtggtgggt agtgtattaa tcgtgccggg cggcagccgc
     4561 gatttccaga tcgtcaacct cgtcgcccaa actttcttca gcagccaatc cagttcgggc
     4621 tactgcaaga atgtcgcacc tggcctcatc ggtacgggaa ccttcatttg gcctattcag
     4681 cagcattggg tctccggcaa taactatgca ccatggcatc cgggtattga cttggctgct
     4741 cataccggcg atcaggtcta tgctgtcgac aatggcatag tcatctacgc gggctggaat
     4801 aactccggct atggcaatct ggtcgtgatc gatcacggca acggctggat cagttactat
     4861 gcccacaaca gcatcattct agttacctgc ggtcaaaacg tggcacaagg caccgtcatc
     4921 tcagccgccg gatcgactgg ccgctcgacg ggaccgcacc tccacatcga aacgcgcttc
     4981 aatgacgtgc cgcaaaaccc gttcaacgtc ttgcccccgc cttagcgctg gcgcttctgg
     5041 ctaattgcgg tatactcgcc tcgaacacag ctgttgtgct ggaccactca cagtcttacc
     5101 gggttactcg acaagcaacg gtgttcatta atcattatgg ctatttctca aaaatcactt
     5161 cggaccaata cccagctcag ggaaagtcgc gcagagtcgc tgttcgattt atctctgccc
     5221 aaaacgttcg ataaccgtgt agcgcatagc atcgccatca tcgcgacacg tttatggcag
     5281 accaccacag ccggtttagt c
//
LOCUS       DASRTJ010000105         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_233020,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000105 DASRTJ010000000
VERSION     DASRTJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233020"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFF70_02185"
     CDS             complement(<1..491)
                     /locus_tag="VFF70_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZY43532.1"
                     /translation="MITIDFARTLYPADADSAHDFDHILRVLHMAEKIAQTEGADIDV
                     VRVAALLHDIGLDQGRAGHEISAANRAREILKNLAKGSATLEDSTQQPSQGYSDEFIE
                     AVAHAIESHRFRSGPAPDTLEAKVLFDADKLDSIGAIGIARAFAFGGWRGQKLWADVP
                     ADYT"
     gene            complement(614..1735)
                     /locus_tag="VFF70_02190"
     CDS             complement(614..1735)
                     /locus_tag="VFF70_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43533.1"
                     /translation="MNSKPVKLLFLFADVGGGHRAPAEAVREALHRTVGDAAHVELID
                     MLADYAPFPYNRGQQTYTQSARFARPLLGFVYHLLNGRLRISLGYSVLWRPMRRATRE
                     CMIDHPADLVVSFHPTYSFPLTWLRFPCAFVFTDLITNHALWCAPGAKNYIVPTNSAR
                     DGALRHGVPADRISVTGLPIHPRFLDPIDHPDDIRRRLGITSDKPIVLLTGGGEGLGR
                     LYDIAQAIGESGLNAQLIIIAGRNNDLMTQLEQTEWPMETRVLGFTRDMPDWLALADI
                     LITKSGSNTIAEALVRGLPMILFDFVPGQEEGNPEYIVKAGAGAYCPRPAHVVSTLRD
                     WLNDRSRLLQMSENARKISRPNAAFNVAKIIFELAKNKM"
     gene            1797..2012
                     /locus_tag="VFF70_02195"
     CDS             1797..2012
                     /locus_tag="VFF70_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43534.1"
                     /translation="MNLIISYRRSAIAMAAGAATRHSHHFSICAFDLRDLFGQGHHAQ
                     EMIEIVGGISLGGMKRAGKIDRDHILA"
     gene            complement(2004..>2444)
                     /locus_tag="VFF70_02200"
     CDS             complement(2004..>2444)
                     /locus_tag="VFF70_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY43535.1"
                     /translation="DQGHTVTTLDIVDKSWVPALTPQIFDGKQLPFADNSFDVALLLT
                     VLHHIPCPDDTLIEARRVAQRIIIVEDVFESLAEKWATWIGDSWLNMEIFGHPHSNRN
                     DQGWRETFARYGLRVLHTHQQFLWFFPFRFRLATYVLSRDDSSG"
BASE COUNT          535 a          647 c          723 g          539 t
ORIGIN      
        1 gtgtaatcgg ccggtacatc ggcccacagt ttctgtccgc gccagccgcc gaaggcgaac
       61 gcgcgcgcga tgccgatcgc gccgatcgag tcgagcttgt cggcgtcgaa cagcactttg
      121 gcctcaagcg tgtccggggc cggacccgat cggaagcggt gtgactcgat ggcgtgagcg
      181 acggcttcga tgaattcatc tgaataacct tgcgaaggtt gttgagtaga atcttctagc
      241 gttgccgaac ccttcgcaag gtttttgaga atctctcgcg cccgattcgc cgccgaaatt
      301 tcatgcccgg cgcggccttg atcgaggccg atgtcgtgca gtaaggctgc gacgcgaacc
      361 acgtcgatat ctgcgccttc agtttgcgca atcttctcag ccatgtgcag tacgcgcagg
      421 atatgatcga aatcgtgcgc ggaatcggca tcggcaggat agagtgtgcg ggcaaaatcg
      481 atcgtgatca tgtttatccg ttaggtaggg gcgaggcatt cgcacacaca ttttgatcgt
      541 aagcagtttt gttgttgcca ccgtgttgat caatgtgggt ttttgggcga atgcctcgcc
      601 cctgcaattc aatttacatt ttattcttcg ccaactcaaa gatgatcttt gcaacattga
      661 aggccgcgtt cgggcgtgaa atcttgcgcg cgttttccga catctgtaac aacctggatc
      721 gatcgttcag ccaatcgcgc agtgtcgaga caacgtgcgc tgggcgcgga caataagcgc
      781 cggctccggc tttgacgatg tattcgggat tgccttcttc ttgccccggc acaaaatcga
      841 acaggatcat cggcaggccg cgcaccaatg cttcggcgat ggtgttcgat ccggatttgg
      901 tgatcaatat atcggccagc gccagccaat ccggcatgtc gcgggtgaag cccagcaccc
      961 tggtttccat aggccactcg gtttgctcca attgtgtcat tagatcgttg ttgcgtccgg
     1021 cgatgatgat cagctgcgcg ttaaggcctg actcaccgat ggcctgcgcg atgtcgtaca
     1081 atcgaccaag gccctcgccg ccgcccgtta ataaaacgat cggtttgtcg ctggtaatgc
     1141 ccaagcgccg cctgatatcg tcggggtgat cgattggatc cagaaagcgc ggatgtatcg
     1201 gcagaccggt cacgctgatc cgatcggccg gaacgccgtg ccgcagtgcg ccatcgcggg
     1261 cagaattcgt cggcacaata tagtttttgg cgcccggcgc gcaccataga gcgtgattgg
     1321 tgatcagatc agtgaacaca aacgcgcacg gaaagcgcag ccaggttagc ggaaaactgt
     1381 aagtcgggtg aaatgagaca acaagatcag ccggatgatc gatcatacat tcacgtgtgg
     1441 cgcggcgcat gggccgccac aatacgctgt aaccaagact aatgcgcagt cgtccgttga
     1501 gcagatgata aacaaagccg agcagtggtc gagcgaagcg cgctgactgc gtgtatgttt
     1561 gctggccgcg attgtacgga aatggcgcgt agtcggccaa catatcaatc aattcaacat
     1621 gcgccgcatc accgacggtg cggtgcaagg cctcacgcac ggcttctgcc ggcgcgcgat
     1681 gcccgccgcc gacatcggca aatagaaaga gaagtttaac gggtttagag ttcatcatct
     1741 atcgggtttg agaaacgatc ggctgggatg gcagattgcg tgcaatggat cgacgattga
     1801 atttgatcat cagctatcga cgatcggcca ttgctatggc ggctggtgca gcaacgcgcc
     1861 actctcacca cttcagcatt tgcgccttcg acctgcgcga tctttttggc caggggcatc
     1921 acgcgcagga gatgatcgaa atcgtgggcg gaatcagcct cggtgggatg aagcgtgcgg
     1981 gcaaaatcga tcgtgatcac attttagcct gatgaatcat cccggctcag cacataggtg
     2041 gcaaggcgga aacggaaggg gaagaaccaa agaaattgtt ggtgagtgtg gagcacacgc
     2101 aagccgtagc gcgcaaaggt ttcgcgccag ccttgatcgt tgcggtttga atgcggatgc
     2161 ccgaaaattt ccatgttcag ccacgaatcg ccaatccacg tcgcccattt ttcggcgagg
     2221 ctctcgaaca catcttcgac gataatgatg cgctgagcga cgcggcgcgc ttcgattagc
     2281 gtatcgtcgg ggcatggaat gtgatgcagc acggtcagca gcagcgcaac gtcgaatgag
     2341 ttgtcggcga atggtagctg cttaccgtcg aagatttgcg gcgttaaggc tggcacccag
     2401 cttttgtcca cgatgtcaag cgtcgtaacg gtgtgaccct ggtc
//
LOCUS       DASRTJ010000106         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_343240,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000106 DASRTJ010000000
VERSION     DASRTJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343240"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..884)
                     /locus_tag="VFF70_02205"
     CDS             complement(87..884)
                     /locus_tag="VFF70_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZY43536.1"
                     /translation="MSRIRLDQLLVERGLVESRERGQRLIMAGEVLINEQIADKPGML
                     VASESVVYVKAPLPFVSRGGLKLAAALDRFAIPIGGAICADVGASTGGFTDCLLQRGA
                     SKVYAIDVGYGQLDWKIRSDTRVVVLDRTNARYLEALPESIEVATIDTAFISLSLILP
                     AVIKWLKPDAHIVALIKPQFEAGRDRVGKGGVVRDPLVHAEVLRSILNFAHKIDLKPI
                     DLIRSPIEGPAGNIEFLVWLRNESNDRTSIDQLIQAVGFNQERGLHT"
     gene            1076..2890
                     /locus_tag="VFF70_02210"
     CDS             1076..2890
                     /locus_tag="VFF70_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43537.1"
                     /translation="MFHRYRDRNEQQTDQPTTISITRGQLRTVIGLVIGNVIVLIALA
                     IIAFNAFNQPPIQIIQVVTQPPPLATRVPTPTPSPTPPPPSPFGGGGSIAFTLRRNGN
                     SDIYAVNAGSNQLIRLINNPADDRAAAYSPDGHGLVFASHRDGNWEIYRMEIASGVAT
                     RLTFSNTYDGAPAWSPDGQSIAFESYRGSNLDIYIMDRDGRSVKRLTTNIAPDMNPAW
                     SPDSKSIAFSSFRNGNKDIYLLSLDSAEGEADVADLTNTPDRDEDNPAWSPDGKQLAY
                     TSSGRTGDQLIYLNTFDLQFKSLQEADIGLFGQGTEPTWSPDGNALAFTYHQGDSDVL
                     IGANIGGWGLAQPAFGGREFIEHPTWRTNLIPTEAISRSLADAPLKAPPLYTEIISPT
                     GPGQPPYAFVQLENIKPSGGTLSDAVDDSFRALRARVMRESGFDYLGTLGDSFRPMNH
                     IPRDGQSRRSYHVAGRAFDIDQTPYTQGNKQVVFVREDIGSITYWRVFIKAKNQDGSM
                     GEPLREAPWNLQVGTDSGGAQITTIPTGYYVDFTTIAADYGWQRVRAIYRWRSFYPDV
                     EWWHFQKTEGVSWWDAMKQVYKENDIIASYGPYPGRDN"
     gene            3172..>3748
                     /locus_tag="VFF70_02215"
     CDS             3172..>3748
                     /locus_tag="VFF70_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43538.1"
                     /translation="MKTQLTLVLLLVSAFALSACGTGAATTTGGTPGATTKPNVAPTS
                     APVIVPTRSTGGNLDLTDVGQGLDTLDSYQTNFTMSFDGTEDGTPKQWNWTTLEEFVK
                     NPAAKHSKISSSNTGVADSSFETWEVNSKIFFMFGTTCSQSDSDTPPTGSSSFTPSSI
                     IGDIQGAQLLGTEAINGIPTQHFAVDVQRYAA"
BASE COUNT          889 a         1076 c          938 g          845 t
ORIGIN      
        1 gatctgaagg atgcgtttga gttgggccgc ttgcagcgcc agagccgtga cgctgcggcg
       61 ttgagggcta gttttcggtt ttgggttcaa gtatgaagcc ctcgctcttg gttgaagccc
      121 actgcttgaa tcaattgatc gatcgaagtc cggtcgttcg attcattacg cagccacact
      181 aagaattcga tgttgcccgc aggcccttca atcggcgagc ggatcagatc gatcggcttg
      241 agatcgatct tgtgcgcaaa gtttaggatc gatctcagga cctcggcgtg cacgagcgga
      301 tcgcgcacca ctccgccctt ccccacccga tcgcgccccg cttcgaattg cggcttgatc
      361 aacgcgacga tgtgcgcgtc cggtttcagc catttaatca ccgccggtaa aatcaaactc
      421 agcgagatga aagcagtatc gatcgttgca acctcaatcg attcgggcag tgcttcaaga
      481 taccgcgcat tcgttcgatc aagtaccacc acccgtgtat cactgcgaat cttccaatcg
      541 agttggccgt agccgacatc gatggcataa actttgctcg cgccgcgctg cagcaaacaa
      601 tcggtgaagc cgccagtcga tgcgcccaca tccgcgcaga tcgcgccacc gattggaatc
      661 gcaaatcgat cgagcgccgc cgctaacttt aatccgccgc ggctcacgaa tggcaatggc
      721 gctttgacat acaccactga ctcggacgct acgagcatgc ccggcttatc ggcaatttgc
      781 tcgttgatca aaacttcacc ggccatgatg aggcgctggc cgcgctcgcg gctttcgacg
      841 aggccgcgct ctaccaataa ttgatccaac cgaatgcgac tcaccccgta agcttattcg
      901 aagtggaaga aaagtcaaga ttaaaattgc gtcctacact tcagggtaca cgcgaatgtc
      961 gtcatggaaa tattgttccc ccgcagtgca actgcaggca tacttcgcag caccttacac
     1021 cgcttcatca tttgacaggc tctaacggcg cgtgtatagt cagcccatgc gctcgttgtt
     1081 tcatcgctat cgcgatcgga atgagcaaca gaccgatcaa ccgaccacca tctcaatcac
     1141 gcgcgggcag ctgcgcaccg tcatcggctt agtgatcggc aatgtgatcg ttttgatcgc
     1201 gttggcgatc atcgccttca acgcatttaa ccagccaccc atccaaatca ttcaagttgt
     1261 gacgcagcca ccgcccctgg cgacacgtgt gcccacgccc acaccatcgc cgacgccacc
     1321 gcccccctca ccctttggcg gcggtggatc gattgcgttc acactgcgcc gcaacggcaa
     1381 ctccgatatt tacgctgtca atgccggtag caatcaatta attcgcttga tcaacaatcc
     1441 cgctgacgat cgcgctgctg cgtattcccc cgacggccat ggcctggtgt ttgcctcgca
     1501 tcgtgatggc aactgggaaa tttatcgcat ggagatcgcc agcggcgtcg ccacacgact
     1561 caccttcagc aacacatatg atggcgcacc ggcgtggtcg cccgatggtc agtctatcgc
     1621 gtttgaatcg tatcgaggca gcaatctgga tatctatatt atggatcgcg atggtcgcag
     1681 cgtcaaacgt ttgactacca atatcgcgcc cgatatgaat cccgcttggt cacctgatag
     1741 caaatcgatc gcgttctcgt catttcggaa tgggaataag gacatctatc tgttatcgct
     1801 cgattcagct gaaggcgaag cggatgtagc cgatttgacc aacacacccg atcgggatga
     1861 agataatcct gcgtggtcgc cggatggcaa gcaactcgcc tatacatctt caggccggac
     1921 aggcgatcaa ttgatctacc tgaacacgtt cgatctgcaa ttcaagagtt tgcaagaagc
     1981 cgatatcggc ctgttcggtc aaggcactga gccgacgtgg tcgcccgacg ggaatgctct
     2041 ggctttcacc tatcatcaag gcgacagtga tgtgctgatc ggcgcaaaca taggcggctg
     2101 gggtctggcc cagcctgcct tcggcggccg ggagtttatc gaacatccta cttggagaac
     2161 gaatctcatt ccaaccgaag cgatcagccg cagcttggct gacgctccgt tgaaagcgcc
     2221 gccattgtat acggaaatca ttagtccgac cggacccggc caaccaccgt atgctttcgt
     2281 tcaacttgaa aatatcaagc cctctggcgg cacattgagc gatgcggtcg atgattcatt
     2341 ccgcgcgctg cgcgcacgtg tcatgcgcga atccggcttt gattatctcg gcacgctcgg
     2401 cgattcattt cgtccgatga atcatatccc gcgcgacggg caaagccgcc gatcgtatca
     2461 tgtggctggc cgggcatttg atatcgatca aacgccatat actcaaggca acaaacaagt
     2521 tgtctttgta cgtgaggaca ttggctcaat cacctactgg cgcgttttta tcaaagccaa
     2581 aaatcaagac ggcagtatgg gcgagccgct gcgtgaagca ccgtggaatt tgcaagtcgg
     2641 cacggatagc ggcggcgcgc aaataaccac gattcctacc ggttactacg tcgatttcac
     2701 aaccatcgct gccgattatg gctggcaacg tgtgcgagcg atttatcgct ggcgatcttt
     2761 ctatcccgac gtcgaatggt ggcactttca aaaaaccgaa ggcgtgagtt ggtgggatgc
     2821 catgaaacaa gtgtataagg aaaacgacat tattgcctca tatggaccat atccaggtcg
     2881 tgataattaa gccgtcaact ttgatcgtca cccacggtct taacaaagtc gattgacaat
     2941 ttgtttggcg cgtgctctcg cggctcaggc agtgtcaccc tgagcgcagc gaaaggtccc
     3001 tatccattga cgcagagatt ctttgcttcg ctcagaatga cagtcagcga ctttgttaaa
     3061 gcactagatt gtcaccctcg cagagttgac accactttca ggtgtgttaa gattcaactc
     3121 gcatagtcaa attgtaaatc acgattctaa agaattcagg gaggagaata tatgaagact
     3181 caattaacgc tcgtcttgtt actcgtcagc gcctttgcct taagcgcatg cggaaccgga
     3241 gcggcaacga ctactggcgg cacacccggt gccaccacaa aaccgaatgt cgcgcccaca
     3301 agcgcaccgg tcatcgttcc gactcgatcg acgggcggca atctcgatct aaccgacgtt
     3361 ggacaaggct tagatacgct agattcgtat caaaccaact tcaccatgtc atttgatggc
     3421 accgaagatg gcacgcccaa gcaatggaat tggaccacgc tcgaagaatt cgttaaaaat
     3481 ccagcggcta aacactcaaa gatctccagc agcaataccg gtgttgccga ctccagcttt
     3541 gaaacatggg aagtgaacag caagattttc ttcatgttcg gcacaacctg ctcccaatcc
     3601 gattccgata ccccgccgac tggcagcagc agctttacac cgtccagcat catcggcgat
     3661 attcaaggcg cgcagctgct cggtacagaa gccatcaatg gtattccgac tcagcacttc
     3721 gcggtcgatg tgcagcgcta tgcggctt
//
LOCUS       DASRTJ010000107         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_267587,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000107 DASRTJ010000000
VERSION     DASRTJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267587"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFF70_02220"
     CDS             <1..512
                     /locus_tag="VFF70_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZY43539.1"
                     /translation="LNFCPRCGNALEDQFKFGRMRRVCPNCGFIFFQDPKVAAAVLAE
                     RDGRVALVKRAVDPRKGAWALPAGFVEIDESPIETAARECLEETGLIAQVHDVLGVFH
                     GRDNPNSPVILIVYWAKIIGGELKADDDVDEVRLFAPDEIPDSLAFESTQVALERWKQ
                     LQIQKTAKS"
     gene            complement(532..903)
                     /locus_tag="VFF70_02225"
     CDS             complement(532..903)
                     /locus_tag="VFF70_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZY43540.1"
                     /translation="MLPRAEFEQLVAQALDDLPIEFQQAMRNIEVLIEAWPTQTDLRT
                     TGLVQGQTLLGLYRGIPLTDRTGGYAMVSPDTITIYQGPIEQAYRTAQAIRDGVKHTV
                     IHELAHHFGIDDDRLRELGAY"
     gene            complement(957..1685)
                     /locus_tag="VFF70_02230"
     CDS             complement(957..1685)
                     /locus_tag="VFF70_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZY43541.1"
                     /translation="MAIGLDDYFVDRELTPRDENGEWDYEAFGAVDHTLFSQQLRELT
                     DGQMVMLPRYDFKSGKRATGSTVRISRDHIIIAEGIHALNPNLVPDVPPERVYRIYVS
                     ALTQLNIDQHNRISTTDTRLVRRIVRDARERGYSARDTISRWDKVRAGESRNIFPFQE
                     NANVMFNSALVYEHTALKSLAEPLLRQIRSGTPEYMEADRLLAFLEWFRSAPIDFVPD
                     NSILREFIGGSSLKDFKLWESTNQ"
BASE COUNT          384 a          465 c          461 g          397 t
ORIGIN      
        1 atctcaactt ctgtccgcgc tgcggaaacg cgctggaaga tcaatttaaa tttggccgca
       61 tgcggcgagt gtgtcccaac tgcggtttta ttttctttca agatcccaaa gtcgctgccg
      121 ccgtcctggc tgaacgcgat ggtcgggtcg ccttagtcaa acgcgcggtc gatccacgca
      181 aaggcgcgtg ggcgctaccc gccggtttcg tcgagatcga tgaaagcccg atcgaaacgg
      241 cggcgcgtga atgcctggaa gaaacggggc tcattgcgca agtccatgac gtgttaggcg
      301 tctttcatgg ccgcgacaat ccgaacagcc cggtgatttt gatcgtctat tgggcgaaga
      361 tcatcggcgg agaattgaaa gccgatgatg atgtagatga agtgcgctta ttcgcgcccg
      421 atgaaattcc agatagcctc gcgtttgagt cgacgcaggt tgcattggaa agatggaaac
      481 agctacaaat ccaaaaaacg gccaagagtt gaacccttgg ccgtttgcct tttaatacgc
      541 ccctaactcc cgcaatcgat cgtcgtcaat cccaaagtga tgagccagtt catgaatcac
      601 cgtgtgcttc acgccgtcgc gaatggcctg cgccgtgcgg taggcttgct cgatcggccc
      661 ctgatagatt gtgatcgtat cgggtgaaac catggcatag ccgcccgtcc gatcggtcaa
      721 cggaatcccg cgatacaaac ccagcagtgt ttgaccttgc acgaggccgg tggtgcgcag
      781 atcagtctgc gtcggccacg cttcgatcag cacttcgatg ttgcgcatcg cttgttgaaa
      841 ttcgatcggg agatcgtcca gggcttgggc gacgagttgt tcaaattcgg cacggggcag
      901 catggtagca atctacaact ttcgatcgtc cctgaaggca aatcgattat tgttcattat
      961 tggtttgtcg attcccacag cttgaaatct ttcaaactcg atccgccaat gaactccctg
     1021 aggatcgaat tgtcggggac gaaatcaatc ggcgcggagc ggaaccattc gaggaacgcc
     1081 aacaaccgat cggcctccat atattccggt gtgcccgatc gaatttggcg cagtagcggt
     1141 tcggccaacg actttaatgc ggtgtgttca tagaccagcg ccgagttgaa catgacattc
     1201 gcattttctt gaaacggaaa aatattgcgg ctttcacccg cgcggacttt atcccaacgg
     1261 ctgatcgtat cccgcgccga ataaccgcgc tcacgcgcat cacgcacaat gcgccgcacc
     1321 agccgcgtat cggtggtgct gatgcgattg tgttgatcga tgttgagctg cgtcaaagcc
     1381 gaaacgtaaa tgcggtacac gcgttccggc ggaacatccg gcaccaaatt cggattcagc
     1441 gcgtgaatgc cttcggcaat gataatgtga tcgcgcgaga tgcgcactgt tgatccggtc
     1501 gcccgcttgc cacttttgaa atcgtaccgc ggcagcatca ccatctggcc atccgtgagt
     1561 tcgcgcagtt gttggctgaa caacgtatga tcgaccgcgc cgaacgcctc gtaatcccat
     1621 tcgccatttt catcgcgcgg tgttaattca cgatcgacaa aataatcatc cagcccgatc
     1681 gcgatcgggc tggatgatta ttttgtc
//
LOCUS       DASRTJ010000108         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_233086,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000108 DASRTJ010000000
VERSION     DASRTJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233086"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFF70_02235"
     CDS             <1..725
                     /locus_tag="VFF70_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43542.1"
                     /translation="QASYAADVAKMAAAGADAVEIWNEPNIDREWPNGQISGGNYTAL
                     LAKAYPAIKAANPGTLVISAAPAPTGYFAGGCQAAGCNDDIFLQQMAAAGAANYMDCI
                     GAHHNAGTTAPDVSSGRPEGNHYSWYFLPTLNLYYGSFGGARKVCFTELGYLSPEGYP
                     SLQSTAPGFAWAENTTIAQQAAWLAQAASMSANSGKVRLVIIFNVDFDYYGSDPQAGY
                     AIIRKGGGCPACESLGAVMGAH"
     gene            818..2152
                     /locus_tag="VFF70_02240"
     CDS             818..2152
                     /locus_tag="VFF70_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZY43543.1"
                     /translation="MNNKSFFDRIPLPVLIAAWLIAVLLGVLLVFSQFFNTSSSITAQ
                     SAPTKVANVPPVTQIPQATSLAPKPTSPVPQATPTIPALQDPAFGYGIQADFLTGDPA
                     YAMTQTARLRMGWIKQQLRWGVFSPAPDQFDWSGFDRVINAASAKGLKVMLSIVTAPQ
                     WSHPNLAATKDDPEAINGPPDDLTAYAKFVGTVVDRYPGQVHAIEIWNEQNIDAEWRT
                     APQVVSPEKYAEMLKLAYQTIKAKDPNIIVISGALAPTGYFVGGCTEAGCDDGPYLKR
                     LVDAGFLPYVDCVGVHANGYNVPPDKTQADGYTDPAATFKGPFTNPIPSWYFRGTLEL
                     YRDTVQNQKPLCVTEFGWATNDGFGVVVPGYEFASDNTQQEQADWIVQAYTLMKQWGY
                     VKLAFLFNLDYAQKSPNGANDRTADWSITGLDGSARPAFNALHDSMKAMSGQ"
     gene            2181..3302
                     /locus_tag="VFF70_02245"
     CDS             2181..3302
                     /locus_tag="VFF70_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZY43544.1"
                     /translation="MQKKLIGFIVIAQFLVACGNPQATIVPALVPIHLKSPEYGLQAF
                     LWWKPEITKRDLTLIQQMGFTWVKQNIAWRDIEIAKAEYSWSLADDMVRRVGRRVGLK
                     LLIRLDRQPFWSQAPNTDPLDNAPPANLNDFGSFCHAIADRFKGQIAAYEVWNEPNLA
                     REWGNQPPNPNQYVELLKGCYNGIKSADPKAIVISAGMAPTGTSDQTAMPDDQFIDQM
                     YQAGAANYFDILGVNAPGYKAPPEISPADVAARSDMGGQRFFAFRHVEDIRAIMQKYH
                     DDQKQIAIMEFGWTTDPIHPAYAWHAVTEQQQADYLVRAYKYATDHWSPWISIMSALT
                     LPDPFWTPDDEQYWWAITYPDWPENKVRPAYDALKAMPK"
     gene            complement(3321..>3500)
                     /locus_tag="VFF70_02250"
     CDS             complement(3321..>3500)
                     /locus_tag="VFF70_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43545.1"
                     /translation="AHTGGHPDLVHLAGIPIAALLFIRALTTSSIRSALGAALMLGFV
                     ATHLAITLQFLVRMR"
BASE COUNT          820 a          977 c          949 g          754 t
ORIGIN      
        1 atcaagcttc atacgccgcc gatgtggcga agatggcggc cgcaggcgct gacgcggtcg
       61 agatttggaa cgaacccaat atcgatcgtg aatggccgaa tggtcagatc agcggcggca
      121 attacacggc cttgttggcc aaagcctacc ctgcgatcaa agccgcgaat cctgggacgc
      181 ttgtcatcag cgccgcacct gcgccgactg gctattttgc tggcggttgt caagcagctg
      241 gctgcaatga cgatattttc ttgcaacaaa tggcggctgc cggtgcggcc aactacatgg
      301 attgcatcgg cgcacatcac aatgcgggca ccactgcgcc ggatgtttcc tccggtcgac
      361 cggaaggcaa tcactacagc tggtacttcc tgccaacgtt gaatctgtat tacggttcat
      421 tcggcggcgc gcgcaaagtg tgcttcactg aattaggtta tctatcgccg gaaggttatc
      481 cctccttgca atcgactgcg ccgggcttcg cgtgggcgga gaacacaacg atcgcgcaac
      541 aagctgcctg gctggcgcaa gccgcatcga tgtcggcgaa cagcggcaaa gtgcgcttgg
      601 tgattatctt caacgtcgac ttcgactact acggcagcga tcctcaagcc ggctatgcga
      661 tcatccgcaa gggcggcggc tgtccggctt gcgaatcact cggtgcggtg atgggcgctc
      721 actaaatcaa tgcagaatgc attttgctta atgcacagtt gctcgtggtc atcgcgattg
      781 gaggaattgt gcattaagaa ttgagaattg aatttttatg aacaacaaat cctttttcga
      841 tcgcatccct ctgcccgtat tgatcgcagc ctggttaatc gccgtattgt taggcgtgct
      901 tttagttttt tcgcagttct tcaacacttc gtcgagcatc accgcacaat cggcgccgac
      961 aaaagtcgct aacgttccac ccgtcactca gattccgcaa gcgaccagcc tcgcgcccaa
     1021 gcccaccagt ccggtgcccc aagcgacacc gactattccc gcgctgcaag atcctgcttt
     1081 cggctatggt attcaagcag atttcctaac cggcgatccg gcttacgcca tgacccagac
     1141 ggctcgcttg cgtatgggct ggatcaaaca acagctgcgc tggggtgtgt tctcgccagc
     1201 accggatcaa ttcgattgga gcggctttga tcgagtgatc aatgcggcca gcgccaaagg
     1261 cttgaaggtg atgctgagta ttgtcaccgc accgcagtgg tcgcatccga atctggcggc
     1321 gacgaaggac gatcccgaag cgatcaacgg cccacccgat gacctcacag cgtatgctaa
     1381 gttcgtcggc acggtcgtcg atcgctaccc gggccaagtc cacgcgatcg aaatttggaa
     1441 cgagcaaaac attgacgcgg aatggcgcac cgcaccgcaa gtggtcagtc ccgaaaaata
     1501 cgccgagatg ttgaagttgg cgtatcaaac gatcaaagcc aaagacccga acatcatcgt
     1561 gatcagcggc gcattggcgc ccaccggtta cttcgtcggc ggctgcaccg aagcgggctg
     1621 cgatgacggg ccgtatctga agcgcttggt cgatgctggt ttcttgccat acgtcgattg
     1681 cgtcggcgtg catgccaacg gctataacgt cccgcccgat aaaactcaag ccgatggcta
     1741 cacggatccg gcggcaactt tcaaaggtcc gttcacgaat ccaatcccgt cgtggtattt
     1801 tcgtggcacg ttggagttgt atcgggatac agttcaaaat caaaaaccat tatgcgtcac
     1861 cgagttcggt tgggcgacca atgacggctt cggcgtagtc gtgcccggtt atgaattcgc
     1921 ctcagacaac acgcaacaag agcaagccga ctggatcgtt caagcgtata cgttgatgaa
     1981 gcaatggggc tacgttaagt tggcgttctt gttcaacctc gattacgcgc agaagtcgcc
     2041 gaacggcgca aacgatcgca ctgcggactg gagcatcacc ggtttggacg gatcggcgcg
     2101 tcccgcgttc aacgcgctgc acgattccat gaaagcgatg tcaggccaat agcgatcgga
     2161 ctgagttgat ccatcgagta atgcagaaaa aactaatcgg ctttattgtc atcgctcaat
     2221 tcctcgtagc ctgcggcaac ccgcaggcta cgatcgttcc tgccctagtg cccattcatc
     2281 tcaaatcgcc ggagtatggc ctgcaagcgt tcttgtggtg gaagccggaa atcaccaaac
     2341 gcgatctcac actgattcaa caaatgggct tcacctgggt caagcaaaat attgcctggc
     2401 gtgatattga aattgcaaag gctgaataca gttggtcatt ggccgatgat atggtgcgcc
     2461 gcgtcggccg gcgcgttggg cttaagctgc tcatccgatt ggatcgtcaa cccttttgga
     2521 gccaagcgcc caacaccgat ccattagaca acgccccacc cgcaaatctc aatgacttcg
     2581 gctcgttttg ccatgccatt gccgatcgat tcaaaggtca gatcgccgcg tatgaagttt
     2641 ggaatgaacc gaacctcgcg cgcgaatggg gcaatcagcc gccgaatccc aatcagtatg
     2701 tggaactatt aaaaggttgc tataacggta tcaagtccgc ggatccgaag gcgatcgtca
     2761 tcagcgcggg catggcgccc accggcacgt ccgatcaaac tgccatgccc gacgatcagt
     2821 ttatcgatca gatgtatcaa gcgggtgcgg ctaattactt tgatattctc ggcgtgaatg
     2881 cgccgggtta taaagcgccg ccggaaattt caccggcgga tgtcgccgct cgatcggata
     2941 tgggcggcca acgcttcttc gcatttcgcc acgtcgaaga tatccgtgct attatgcaaa
     3001 agtatcacga cgatcaaaaa cagatcgcga ttatggaatt cggctggacg accgatccga
     3061 tccatcctgc ttatgcgtgg catgccgtca cggaacagca gcaagcggac tatctggtgc
     3121 gcgcttacaa atatgcgacc gatcattggt cgccgtggat cagcatcatg tcggccttga
     3181 ccttgcccga tccattttgg acaccggacg atgaacaata ctggtgggcg atcacctatc
     3241 ccgattggcc ggaaaacaaa gtgcgccccg cgtatgacgc gttgaaggcg atgccgaaat
     3301 gacaaaagta gaattggaaa ttacctcatt ctaacaagaa actgcaacgt tattgcgaga
     3361 tgcgtagcga cgaagcccaa catcaaagcc gcgcccagcg ccgatcgaat gctcgaggtg
     3421 gtcaacgcgc ggatgaacaa cagcgcggcg atcggaatcc cggccagatg aaccagatca
     3481 ggatgtccgc cggtgtgggc
//
LOCUS       DASRTJ010000109         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_44114,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000109 DASRTJ010000000
VERSION     DASRTJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44114"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFF70_02255"
     CDS             <1..1059
                     /locus_tag="VFF70_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43546.1"
                     /translation="LLIYGPIDLTNYRAAVMTASYFLDSAPSDLFGVAASTDGANFDA
                     LSADSSQDPALSVTHTAIYDLHAYKQKPAVWIAFYFVSNSDDSVGRGAFVDAMVIRAL
                     PMVKIYLPIIGGAPAAPAQTFLYSYTFGSGLNTNSDFLTWGGTYDESCGTDCTRWGQS
                     VNTAGHPNGAMNLYLTTFNDIAAASPNVTATTNYTYNADIYVVQGKGSARFGLIFGAS
                     LSTFDRDGNGDPTFDPNRNFYKFDLQFLDTDNTVVSDYRLSKCQNSFVGCTVIVQKTA
                     VPGGLVGNSGTWNNVTIIRNGNAITGKVNGTQLFSITDSTFTGERKFGIFVHSKDLNS
                     AGNPLKIRFDNVSIMALP"
     gene            1080..2174
                     /locus_tag="VFF70_02260"
     CDS             1080..2174
                     /locus_tag="VFF70_02260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZY43547.1"
                     /translation="MNIPGANPHAQYLSHKVEIDAAIFAAIEGNRYILGPQTHAFEDE
                     FARYIGVKHSVGVGSGTEALHIAICACEIGAGDEVITVSHTAVATVSSIELAGATPVL
                     VDIDPATYTIDPRKIEAAITLHTKAIIPVHIYGGVVDLDPILKIAKQHHLKVIEDCAQ
                     AHGALYHNRRVGSWGDMACYSFYPTKNLGAIGDGGLVATNDASLAERLDLLRQYGWRE
                     RYVSEISGWNTRLDEIQAAILRVKLRHLDDDNNLRRQRAVMYDEMLQGIVTSPIELAN
                     TRSVYHLYVIRHPQRDRLMKHLSDLGIGTAIHYPMPIHLQPAYRGRLGDTGSLPETEK
                     AANEILSLPMFPELAEGDVKIVADAIRAFR"
BASE COUNT          548 a          615 c          591 g          525 t
ORIGIN      
        1 ctattgatct atggcccgat cgatctcacc aattaccgcg cggcggtgat gacggccagc
       61 tatttcctag attcggcgcc aagcgatctc tttggcgtag ctgcttcgac cgatggcgcg
      121 aacttcgatg cgttatccgc ggacagttcg caagatccag cgctgagtgt tacccatact
      181 gccatttacg atctccacgc ttacaagcag aagccggcag tttggatcgc attctatttc
      241 gtcagcaact ccgacgattc tgttggacgc ggtgcatttg tagacgctat ggtcatacgc
      301 gcactgccaa tggtgaaaat ctacttaccg atcatcggcg gcgcgcccgc cgcaccggcc
      361 caaacctttc tttacagtta cacattcggc tcaggactga acaccaactc agatttcctg
      421 acttggggcg ggacttatga tgaaagctgc ggaaccgact gtacgcgctg ggggcaaagc
      481 gtcaacaccg caggccatcc aaacggcgcc atgaacttgt acctcaccac ctttaacgat
      541 atagccgccg cgtcgccgaa tgtcactgcg accactaact acacttacaa cgccgatata
      601 tatgtggtgc agggcaaagg cagtgcgcgt ttcggtctaa tctttggcgc aagtctatcc
      661 acgttcgatc gagacggcaa tggcgatccg acctttgatc cgaatcgaaa cttctataaa
      721 ttcgatctgc aattcttaga cacggataat accgtcgtga gcgattatcg gctgtcgaaa
      781 tgccagaata gttttgttgg ctgcacggtg atcgttcaaa agacggcagt gccgggcggc
      841 ttagtcggca actcgggcac gtggaacaat gtcactatca tccgcaacgg caatgccatc
      901 accggcaaag tgaacgggac acaactcttt agcattaccg acagtacctt caccggtgag
      961 cgcaagtttg gtattttcgt gcactcgaaa gatttgaaca gtgccggcaa tccgctgaag
     1021 attcgctttg acaacgtaag catcatggcg ctgccgtaaa tcgatccgga gatcaacaca
     1081 tgaacatccc tggagctaat ccgcatgcgc agtatctcag ccacaaagtc gagatcgatg
     1141 cagccatatt tgcagctatc gaaggcaatc gctatatcct cggcccacag actcatgcct
     1201 ttgaagatga gttcgcgcgc tacatcggcg tgaagcatag tgtaggagtc ggctcaggca
     1261 ctgaagcatt gcacatcgcc atttgcgcct gcgagattgg cgcgggcgac gaagtaatca
     1321 cagtgtcgca tactgccgtt gcgaccgtat cctcgatcga gttagcgggc gcgacgccag
     1381 tgctcgttga tatcgatccg gcgacttata caatcgatcc tcgcaaaatt gaagccgcca
     1441 tcacgctgca caccaaggcg atcatcccgg ttcacatcta tggcggcgtg gtcgatctcg
     1501 atccgattct gaaaatcgct aagcagcatc atctcaaagt catcgaggat tgcgcgcaag
     1561 cacacggcgc gctgtatcac aatcgacgcg tgggtagttg gggtgatatg gcttgttaca
     1621 gtttttatcc gactaaaaac ttgggcgcga tcggcgatgg gggtttagtg gcaacgaatg
     1681 atgcgtcgct tgccgagcgt ttagatttgc tgcgccaata tggttggcgc gaaagatacg
     1741 ttagtgagat cagcggttgg aacacacgct tagatgaaat acaagcggcg atcctgcgcg
     1801 tgaagctgcg ccatctcgac gacgacaaca atctgcgccg acagcgcgcg gtcatgtacg
     1861 atgaaatgct acaggggatt gtcacctcac cgatcgagct ggcgaacacc cgatcggttt
     1921 accacctgta tgtgattcgt catccgcagc gcgatcgatt gatgaagcat ctaagcgatc
     1981 tagggatcgg cacggcgatt cattacccca tgcctatcca tctgcagcca gcctatcgcg
     2041 gccgcttagg cgataccggc tcgctgcctg aaacggaaaa ggctgcaaac gaaattttgt
     2101 cgctgccgat gtttcccgaa ttggcggaag gtgatgtaaa gatcgtcgcg gatgcgatcc
     2161 gcgcatttcg ataaatcttg ttattgtgtc aaacccacca aagatactgg atatcatcaa
     2221 cttttgtagg tgtgtcaatg cgatgcctcc cgcgagcact ccggtttaga gcaaatctt
//
LOCUS       DASRTJ010000110         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_191852,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000110 DASRTJ010000000
VERSION     DASRTJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191852"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFF70_02265"
     CDS             <1..598
                     /locus_tag="VFF70_02265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43548.1"
                     /translation="LDHSWVYTQDRSTRASLEKFGYHIDRTFHRMQIEMDAPPSLAPI
                     PDGIQIRSFRLGVEDRSMYDAYEEAQADEWGHQWLPFDKWQHYFIGTEDQFDPSLWFL
                     AVEGDQIAGYIFARLERPGDPEEGHIRYVAVRRAWRRRGIAIALLRCAFAELFRRGKR
                     KVGLGVDTTSLTGADRLYERAGMRVYQTTLIYKKILEP"
     gene            complement(639..>2017)
                     /locus_tag="VFF70_02270"
     CDS             complement(639..>2017)
                     /locus_tag="VFF70_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZY43549.1"
                     /translation="SNDAWVNFADGLNLRDKPGGNLLSVIPNGQHLTVLGSPNPPDIN
                     GNVFQQVQTDNGQTGYVAAIYQGSSLLSTTKPATTTPTTPTTSTTDIWVNSDIGLNFR
                     DVPGGNLIAVLPNGQHLTAIGSSNPPDANGTVFQHVRTNDNQTGYVAAIFQGSSLLST
                     TKPVATVTVVPWGKCYVGLGMADPRPFNPADKDATKHSKVEAFKILTLGDADETKQLI
                     NDLKKIKSDMFIVARLFFSTSAGSKFSAQDFVDYCRVGMEACYQEGIGYFEVHNEPNL
                     PDEGFGGNWVNGAAFGAWLTQVLGLLRVKYPSAKFGYPGLSPQSGNAYDAAAFLNGSA
                     SAIALCDWIGVHCYWQTVNGPIYPMSGETDGMYWKRYRTQFPDKLLMITEFSNNIDTV
                     ADDEKGRQYARYFQLLRHENNLGAAFSFAVNWPGQDIHREGWVYKGSENRIGSTLGTL
                     IGQAGYLW"
BASE COUNT          458 a          532 c          549 g          478 t
ORIGIN      
        1 actcgatcat tcttgggtgt acacacaaga tcgatcgacg cgcgcgtcgc tggagaagtt
       61 cggctatcac atcgatcgaa cttttcaccg catgcagatt gagatggatg cgccgccatc
      121 cctcgcgccg attccagatg gaatccaaat tcgatcgttt cgattgggcg tagaagatcg
      181 atcgatgtat gacgcgtacg aagaagcgca ggccgacgag tggggccatc aatggttgcc
      241 gtttgataaa tggcagcatt acttcatcgg caccgaagat caattcgatc cgtcgttgtg
      301 gttcctggcg gtcgagggcg atcagatcgc aggctacatt ttcgcgcgct tggaacgtcc
      361 cggcgatccg gaagaaggcc acatccgtta tgtggcagtc cggcgagcgt ggcgtcgtcg
      421 tggaattgcg atcgctttgc tgcgatgcgc gttcgccgaa ttatttcgac gcggcaaacg
      481 caaagtgggt ttaggcgtcg atacgaccag cctgaccggc gcggatcgat tgtatgaacg
      541 ggcgggcatg cgcgtatatc aaacgacgtt gatctataag aaaattcttg aaccataaaa
      601 tgcaggggca agaagatctc gcccctacgt gaaatgtctt accacaaata ccccgcctga
      661 ccgatcaacg ttcccagtgt gctgccgatg cgattctcgc tgcccttgta gacccagccc
      721 tcgcgatgaa tgtcttgccc cggccagttg acggcaaatg aaaacgccgc gccgaggttg
      781 ttctcatggc gcagcagttg aaagtaacgc gcatactgcc ggcccttctc atcatcggcg
      841 accgtatcga tgttgttgct gaattcagta atcatcagca gcttgtcagg gaattgggtg
      901 cggtatcgtt tccaatacat gccgtccgtc tcgccgctca tgggatagat cgggccgttg
      961 acggtctgcc agtagcaatg cacgccgatc caatcacaca acgctattgc actcgctgat
     1021 ccatttagaa acgcggcggc atcgtaggca ttgccggatt gcggcgatag gccgggatag
     1081 ccaaactttg cactgggata tttcacgcgc aacaaaccta aaacttgagt cagccatgcg
     1141 ccgaaggctg cgccattgac ccagtttccg ccgaagcctt catccggcag gttgggttcg
     1201 ttatgcactt cgaagtatcc aatcccctct tgataacacg cctccatacc gacgcggcaa
     1261 tagtcgacaa agtcctgcgc tgaaaatttg ctgccggcgc tggtcgagaa aaataatcgg
     1321 gcgacaatga acatatccga tttgattttc ttaagatcgt tgatcaattg cttcgtctca
     1381 tcggcatcgc ccaaggtcaa tattttgaag gcttcaacct tgctgtgctt ggtcgcgtct
     1441 ttgtcggctg gattgaacgg gcgcggatcg gccatgccca gaccaacata gcatttgccc
     1501 cacggcacga ccgtgacagt cgccaccggc ttggtggtgc tgagcaaact cgatccttga
     1561 aagatcgcgg ccacatagcc tgtttgatta tcattggttc gaacgtgctg aaacacagtt
     1621 ccattcgcat ccggcggatt ggatgagccg atcgcggtga ggtgctgccc attgggcagg
     1681 accgcgatca aattgccgcc cggtacgtcg cgaaaattca agccgatatc cgaattgacc
     1741 catatatcgg ttgttgaagt cgtcggcgtt gttggggtgg tggtcgcagg tttggtggtg
     1801 ctgagcaaac tcgatccttg atagatggcg gccacgtaac cggtttgacc attatcagtt
     1861 tgaacttgtt gaaacacatt tccattaata tccggcggat taggcgatcc aagaacggtc
     1921 aggtgttgtc cattcggaat gacggataat aaattgccgc ccggcttgtc acgcaagttc
     1981 aaaccgtcag cgaagttgac ccacgcatca ttggatg
//
LOCUS       DASRTJ010000111         5008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_6277,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000111 DASRTJ010000000
VERSION     DASRTJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6277"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFF70_02275"
     CDS             complement(<1..216)
                     /locus_tag="VFF70_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZY43550.1"
                     /translation="MLDTLFAPRGIAVIGASRDESKLGYGVARNLVESGYQGGIYFIN
                     PKADQILSHACYPSIEAAPDPIDLVVVI"
     gene            307..564
                     /locus_tag="VFF70_02280"
     CDS             307..564
                     /locus_tag="VFF70_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43551.1"
                     /translation="MENENGAMIDRPVFGFAGSSGIRSNALMRLFSSLPREARSFTHS
                     LTPTAGKRCTARAAPETADQSVSAIAQHARIELSDRAIERI"
     gene            complement(745..915)
                     /locus_tag="VFF70_02285"
     CDS             complement(745..915)
                     /locus_tag="VFF70_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43552.1"
                     /translation="MKRIRSIGILSVVAVVGIIGVATVAAQAQQSQQLHAAPRADTTA
                     PSVGSIVSTTFT"
     gene            complement(1014..1346)
                     /locus_tag="VFF70_02290"
     CDS             complement(1014..1346)
                     /locus_tag="VFF70_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HZY43553.1"
                     /translation="MSNPSVQTWADPAEVASSVYHLLLENDRVRVFDVRFKPGEVAEM
                     HGHPDHVVYVVDDAKLQLATPNGAVQTFDLKAGQTLFMNAGPHETTNVGSHEAHNLEI
                     EFKEDRGI"
     gene            complement(1412..1819)
                     /locus_tag="VFF70_02295"
     CDS             complement(1412..1819)
                     /locus_tag="VFF70_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZY43554.1"
                     /translation="MPTQSQHEPEHEHVKILREIMAQFGTLFDRCPDGIYVYIDDVHK
                     TCNEKLAKMHGTTVDEWQRCESIVDTYAEPEDRAVIVNHYMQTVQGLGSPVRFQYRAR
                     RQDGSVFKAELDMVPFTWGGNSVALHFVREVKK"
     gene            complement(1907..2104)
                     /locus_tag="VFF70_02300"
     CDS             complement(1907..2104)
                     /locus_tag="VFF70_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HZY43555.1"
                     /translation="MLDEKIKKELMEHIRSDVGYPASKQQILAACNNMTMHSPEAKSA
                     AEKLPEQTFQNAGEALKALAL"
     gene            complement(2171..2530)
                     /locus_tag="VFF70_02305"
     CDS             complement(2171..2530)
                     /locus_tag="VFF70_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43556.1"
                     /translation="MLVKTLKWPVISLVVTGMLHLLAEMLLPDLKNSIQPTVIAVILF
                     PFGVWVGYKAIQNGAGFVMAIVFGAILGVLPLALDVVGFGMILGRGVQVGLVSGIFGF
                     LMIVFGSLLGSGFASSE"
     gene            complement(2709..>5008)
                     /locus_tag="VFF70_02310"
     CDS             complement(2709..>5008)
                     /locus_tag="VFF70_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZY43557.1"
                     /translation="FGLKRATRADFLNAIYSLTDGNPFFIEEVLKSLIASGDIYYQDG
                     VWDRKPVSELRIPRSVQDAVQRRSDQLNPQAQEILRLASVAGRRFDFGLLLEVTHMAE
                     TQLLTIVKELIGAQLVVEESADRFAFRHALTQEAAYSTLLKRERKDIHRVVAETLEHL
                     HAPTLEPYWADLAHHFFAAGEWIKALNYSQRVGERAQSLYAPREASEHFSRALIAAEQ
                     LNQQRPDLYRARGATYETLGEFDRARDDFDRALQSARAINDQFAEWQALIDLGALWAG
                     RDYAQTGQYYQHAIDLARSIDQPALIAHSLNRLANWHVNVEQPLEGQQLHTEALIIFE
                     TLKDPEGIAETLDFLGMANYMGGNLVQGTAYYQRAIDLFRALDNRQGLSSSMGAMSIR
                     AATIQTSTMVAAEKLIDAARGCEEAYRVARDIGWRSGEAFALIMWCTTLGPQGDYTQA
                     LDIAHRGIEIAEEIEHHQWMAGGYGILGAIYYDLLALPLARQYLERGLVLGQETGSLH
                     WQRVAAGFLASTLIDLHDLSAAESVLKNALPADSPTHSIGQRLCWCARVELALAQNDP
                     TLALELIDRMTISAPNVIDPLAPNIIRLALLRGEALQQLDRMDEAQKCFNAALAVATE
                     QGNRSLVWRIHLARGKFYQVQAHRSDAEREYLAARSLVDELAVNIADESLRDNFLQAA
                     YALIPIGSPRRSSKQTADGLTPREREVAQHIALGKANREIADELVVGERTIETHVTNI
                     LNKMGFTSRTQIAAWIASKSEKVTG"
BASE COUNT         1181 a         1413 c         1256 g         1158 t
ORIGIN      
        1 aattactacc accagatcga tcggatcggg tgcggcttcg atcgagggat aacaagcgtg
       61 actgagaatt tgatcggctt tgggattgat gaagtagatg ccgccttgat aaccggattc
      121 gaccaagtta cgcgcgaccc cgtaacccag tttgctttca tcgcggctgg cgccgatgac
      181 ggcgataccg cgcggtgcga agagagtgtc aagcataatc gatcctcggg gttgaataaa
      241 ttcagcttac accgattcca tctttcggct aagtgtaagt gatcatacca tcacaagtga
      301 gttgtcatgg aaaatgaaaa cggggcgatg atcgatcgcc ccgttttcgg atttgccggg
      361 agcagcggaa tccgatctaa cgccctgatg agattgtttt cctcgctccc acgcgaagcg
      421 aggtcgttca cgcattcttt aacaccaacg gcaggtaaac gttgtactgc gcgggccgcc
      481 ccagaaaccg ccgatcaaag tgtaagcgcc attgctcagc atgcccgcat cgaactgtcc
      541 gatcgtgcca tcgagcgaat atgatccgcc tgtactgaac ccttcgacta tcgctcagag
      601 caggcttgcc ccaccaccat cgatggtgta ccaactgaga tcgtacttga cagcttcgga
      661 tgaagccgct gcgggatgtg agttgatcga atccagtccg atcagcagca ccaccacgat
      721 tgggatcagg aagagtaagc gcttctaggt gaacgtcgtg ctgacgatcg atcctaccga
      781 tggcgcggtc gtatcggctc ttggagcagc atgaagttgt tgcgattgct gtgcttgagc
      841 cgcgaccgtc gccacgccga tgatgcccac gactgctaca acgcttaaaa tgccgatcga
      901 gcgaatgcgt ttcatattag tctccttgat tcaagcaaag atgatcgggt gagtgactca
      961 cccgatctca tcaacacgct ttccatcaac cccacaacga ttgtgattcg agattaaatt
     1021 ccacgatctt ctttgaattc aatttccaag ttgtgcgcct cgtgtgagcc gacgttcgtc
     1081 gtttcatgcg gcccggcatt cataaacaac gtttgcccgg cttttagatc gaaagtctgc
     1141 acagcgccat ttggcgtcgc caattgaagt ttggcatcat cgacaacata cacgacgtga
     1201 tcgggatggc cgtgcatctc ggcaacttca cccggcttaa aacgcacatc gaacacgcgc
     1261 actcgatcgt tttcgagcaa gagatggtag acactcgatg cgacttcagc gggatcagcc
     1321 cacgtttgaa cagatggatt ggacattggt gtgtttgctt tccttgaata gtctcgtatg
     1381 aagaatttgc gtttcgatcg aggctgaacg gttacttttt cacttcgcgc acgaaatgca
     1441 gcgcaaccga attgccgccc caggtgaacg gtaccatatc caactcggcc ttaaacaccg
     1501 agccatcttg ccgccgggca cggtattgaa atcgaactgg cgagccgaga ccttggacgg
     1561 tctgcatgta atgattcacg atcacagcgc ggtcttcagg ttcggcatac gtatcgacga
     1621 tcgactcgca gcgctgccac tcgtcgacgg tcgtgccgtg catctttgcc aatttctcgt
     1681 tacaagtttt gtgcacatcg tcgatgtaga catagatgcc gtcgggacac cgatcgaaca
     1741 acgtgccgaa ctgagccatg atctcgcgca aaattttgac gtgttcgtgt tcaggttcgt
     1801 gctgagattg ggtcggcatg ttctatctcc ttagactgtg aaaggattaa gccgcccagc
     1861 ggttaggcca aacgacttaa cgaaattttg tgtgatgatc ggagttttac agcgccagag
     1921 ctttaagagc ctcgcccgca ttctggaagg tttgttcggg cagcttttca gcggcactct
     1981 tggcttccgg agaatgcatc gtcatgttat tgcaggccgc cagaatctgc tgcttcgagg
     2041 ctggataacc gacatcactg cggatatgtt ccatcaattc cttcttgatt ttttcatcta
     2101 gcattttaat ctcacctcct ttctcgaaaa aattcttgtt acggctgtag cccttgactc
     2161 acatcctggc ctattcgctc gaagcgaagc cgctgccgag caatgatcca aatacaatca
     2221 tcaagaagcc gaagatgccg ctaaccaagc cgacttgaac gcctcgaccg aggatcatcc
     2281 cgaagccgac gacatccagc gccaatggta gcacgccgag gatcgcgcca aacacaatcg
     2341 ccatcacaaa acccgcgccg ttctgaatcg ctttgtagcc gacccacacg ccgaacggaa
     2401 atagaatgac agcaatcact gtgggctgaa tgctgttctt aagatcgggc agcagcatct
     2461 cggccagtaa gtgcagcatg cccgttacca ccaaactaat caccggccac ttcaaagttt
     2521 tgactaacat tttactttta cctttccttt tgagagagtt ttgattatga gcctactcta
     2581 tcaaaaataa ggtaaagcgt catcgggggt agtacggagg tttcaaaatg gaagtacgga
     2641 gattcaagac ggatgacacg ctgccaaggt gacaaggtga tttgtgcgac attcaccttg
     2701 ccagcacttc accccgtcac cttttcactc tttgaggcga tccatgccgc aatttgcgtt
     2761 cttgaagtga atcccatctt gttgaggata ttcgttacat gcgtttcgat cgtccgttcg
     2821 ccgacgacca actcgtccgc aatctctcga ttcgctttac ccagggcgat atgctgagcc
     2881 acttcgcgtt cgcgcggcgt caagccatcg gctgtctgtt tgctcgatcg gcgcggtgag
     2941 ccgatcggaa tcagcgcata tgcggcttgc aggaagttgt cgcgcagaga ttcatcggcg
     3001 atattcaccg ccaattcatc gacaagtgac cgagccgcaa ggtattcgcg ctcggcatcc
     3061 gatcgatgcg cctggacttg atagaatttg ccacgcgcca aatggatgcg ccagaccagc
     3121 gatcgattgc cctgctcagt cgccacggcc agcgccgcgt taaaacattt ttgggcttcg
     3181 tccatacgat cgagttgctg caatgcctca ccacgcagca gcgctaaacg gataatgtta
     3241 ggcgcaagcg gatcgatcac attaggcgca ctgatcgtca tgcggtcgat caattccagc
     3301 gcgagtgtag gatcattctg cgccaacgcc aattcgacgc gcgcacacca gcacaagcgt
     3361 tgaccgatcg agtgggtagg tgaatcagcg ggcagtgcat ttttcaaaac cgattcggcg
     3421 gctgaaagat catgcagatc gatcaacgtt gacgccagaa atccggcggc cactcgctgc
     3481 cagtgcagcg atccggtttc ttgtcccaag acgagcccgc gctctaaata ttgccgtgcg
     3541 agcggcagcg ccagcagatc gtagtagatc gcaccgagga tgccataacc gccagccatc
     3601 cactggtgat gctcgatctc ttcagcgatc tcaataccgc gatgcgcgat gtccaacgct
     3661 tgcgtataat cgccttgcgg gcctaacgtc gtgcaccaca tgatcaacgc gaacgcttca
     3721 cccgatcgcc aaccaatgtc gcgcgcaact cgatacgcct cttcgcaacc gcgcgctgcg
     3781 tcgatcaatt tctcggcggc caccatcgtg ctggtttgaa tcgtcgctgc gcggatcgac
     3841 atggcaccca tactcgatga caaaccttgg cgattatcca gcgcgcgaaa cagatcgatc
     3901 gcccgctgat aataggccgt gccttgaaca agattaccgc ccatgtaatt ggccatgcct
     3961 aaaaagtcca gcgtctcggc gatgccttct ggatccttca aagtctcgaa aattatcagc
     4021 gcctcagtgt gcagctgctg gccttccaac ggctgttcga cgttgacatg ccaattcgct
     4081 aatcgattca agctgtgcgc aatgagcgcg ggttgatcga tcgagcgggc gagatcgatc
     4141 gcgtgttgat aatactgccc ggtctgcgca taatctcgac cggcccacaa cgcccccaga
     4201 tcgatcaacg cttgccactc ggcaaattga tcgttgatcg cacgcgccga ttgcaaagcc
     4261 cgatcgaaat catcgcgcgc ccgatcgaac tcaccgagtg tttcatatgt cgcaccccgc
     4321 gcgcgataga gatcgggccg ctgttgattc aactgctcag ccgcaatcaa cgcccggctg
     4381 aaatgttcgc tggcttcacg cggcgcatac aacgactgag cccgctcgcc cactcgttga
     4441 gaatagttca aagccttgat ccattcgccc gccgcaaaaa agtgatgcgc gagatccgcc
     4501 caatacggtt ccaaagttgg cgcgtgcaaa tgctccaacg tttcggcgac aacccgatgg
     4561 atatccttgc gttcgcgttt gagcagcgtt gaatacgcgg cctcttgggt gagcgcatgt
     4621 cgaaacgcaa agcgatcggc ggattcttcc accaccagct gtgcgccgat caactctttg
     4681 acgatagtta ataattgggt ttcagccatg tgcgtcacct caagcaataa accaaaatcg
     4741 aagcgtcgtc cggcgaccga agccagccgc aatatttctt gcgcctgcgg gttgagctga
     4801 tccgatcgac gctgaaccgc atcttgtaca ctgcgcggaa tacgcaactc actcaccggc
     4861 ttgcgatccc acacgccatc ttgatagtaa atatcaccgg aagcaatcaa cgacttcaac
     4921 acttcttcaa tgaagaatgg attgccatct gtgagagaat aaattgcatt cagaaaatcg
     4981 gcgcgcgtgg cacgtttcaa cccaaaga
//
LOCUS       DASRTJ010000112         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_116265,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000112 DASRTJ010000000
VERSION     DASRTJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116265"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFF70_02315"
     CDS             <1..1068
                     /locus_tag="VFF70_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZY43558.1"
                     /translation="VDEDLKLIGIVTTRDLLFEADDRKPIAEIMHRAVVTAPPDTSLE
                     EAEKILHANRIEKLPLVDGQQRIAGLITLKDIMKIAQFPQATKDARGRLAVGAAVGVR
                     ANELQRVECLLNAGADCLVVDIAHGDSELEIDMIRQIRERFPQAQIIGGNVATAEGTQ
                     RLIDAGADAVKVGVGPGSICITRVVAGAGVPQLSAVMECAQVAREANVPIIADGGIRQ
                     PGDAAKALAAGAQSIMIGSLLAGTDESPGLMMTRKGHRYKVSRGMASVEANVVRQQRE
                     GDGRVLQEDIDEYVSEGVEAAVPYRGRVKEILIQLVGGIQSGMSYSGAHSIEELFEKA
                     VFVRMSPAGLRESHAHDVEVL"
     gene            1124..1642
                     /locus_tag="VFF70_02320"
     CDS             1124..1642
                     /locus_tag="VFF70_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43559.1"
                     /translation="MQSSGKLIGLMLIAGGLLCGAIGGLWAFTQQAAEKLDSGGLVFA
                     IGGLAIIALPLIFAGIFMLVRGGREDVELQQIAKERKILNMVSTQGQVKIAEVALELN
                     ATRDQIKEWIYDLVGKELFSGYINWDDGILFSKQASQLKTGQCPNCGSKLELAGKGVV
                     KCPACGSEVFLS"
     gene            1668..2297
                     /locus_tag="VFF70_02325"
     CDS             1668..2297
                     /locus_tag="VFF70_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43560.1"
                     /translation="MGSNLTDQLKNDRGGFSKLLSHIPGFKGYMEKETRRDADKLIRD
                     TLVTRFDQQLSRLTGVQNDLIDNGGLDYVDDVEGAAVKLRTFIARVKTAPRGYAGMFD
                     AVHVREEQLDLLYNFDAQLLSEVDHMSSAIDGVSAAVNPTPAVPAAMPVADSSATTGS
                     AAVPVQPSVPAPVMTGPAAIKGAISALQLVCAGLNDLYDKREHVMLGSV"
     gene            2346..>3093
                     /locus_tag="VFF70_02330"
     CDS             2346..>3093
                     /locus_tag="VFF70_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZY43561.1"
                     /translation="MPRIIDVVEFNDESGTEIVHRIPENGSGDFRFGSQVIVREQQNA
                     VFFRDGKSLDVFKAGRHTINTANLPILSGLIGLATSGKTPFTAEVYYVSMRQFLDMGW
                     GTPEPIAFRDADLGMVRLRANGVYSFEIKDPSLFVNKVVGARGIFQTSDIEDMLRSII
                     ISKFIDLLGEAKVPFLDLGTKYNELSAGAKAKISADFEAMGIQLMSYYVRAITPTEET
                     AKALDERASMGAIGDMNKYLQFQAARGIRDA"
BASE COUNT          709 a          739 c          944 g          701 t
ORIGIN      
        1 gtcgatgaag atctgaagtt gatcggcatt gtcaccacgc gcgatctttt atttgaggcc
       61 gacgaccgta agccgatcgc cgagatcatg catcgagcgg ttgtcaccgc gccgccggac
      121 acttcgttgg aagaagcgga aaagattttg catgcgaatc gcatcgagaa gctgccgctg
      181 gtcgatggac agcagcgcat cgccggactg atcacactca aagacattat gaagatcgct
      241 caattcccgc aagccactaa agatgcgcgg ggtcgattgg cggtcggtgc ggcggtcggt
      301 gtgcgggcga atgaactgca acgtgtcgag tgtctgttga atgcgggcgc ggactgcctc
      361 gtggtggata ttgcgcacgg ggattcagaa cttgagatcg atatgattcg tcaaattcga
      421 gagcgcttcc cgcaggcgca gattattggc ggcaatgtcg cgaccgctga aggcacgcag
      481 cgtttgatcg acgcgggcgc agatgcggtc aaagtcggag tcggccccgg ttcgatctgc
      541 atcacgcgtg tcgttgcagg tgcaggtgtg ccgcagctct cagcggtaat ggaatgtgcg
      601 caggttgcgc gggaagccaa cgttccgatt attgcggacg gcggcattcg ccaacccggc
      661 gatgcggcaa aagctttggc agccggtgct caatcgatca tgatcggcag tttgctcgct
      721 ggaacggatg agagtccggg tttgatgatg acgcgcaaag gacatcgcta caaagtctca
      781 cgcggcatgg cttccgttga agcgaatgtc gttcgccaac agcgtgaagg cgatggccgt
      841 gttttacagg aagacatcga cgaatatgtt tcagaaggtg ttgaagcggc ggtgccgtat
      901 cgcggccggg tgaaggaaat tctgatacag ctcgtcggtg gaattcaatc ggggatgagc
      961 tatagcggcg cacattcgat cgaagaatta tttgagaagg ccgtgttcgt gcggatgagt
     1021 cccgctggcc tgcgagaatc acacgcgcac gatgtggaag tgttataagc tgtgggcggt
     1081 tcgggtgtgg tagaatcgca gtgattgatc cttggaggta cgcatgcaga gttccggtaa
     1141 actgatcggc ctgatgttga tcgcaggggg cttgttatgc ggtgcgatcg gcggactgtg
     1201 ggcctttacg caacaggctg cggaaaaact cgatagtggc gggctggtct tcgcgatcgg
     1261 tggactggcg atcatcgcgc tgccattgat ttttgcaggt atcttcatgc tcgtgcgcgg
     1321 cggccgcgaa gatgttgaat tgcagcagat tgctaaagag cgcaaaatcc tgaacatggt
     1381 ttcgacgcag ggtcaagtga agatcgccga ggttgctctg gaattgaatg ccacgcgcga
     1441 tcagatcaag gaatggattt acgatctcgt cggtaaagag ctgttcagtg gctatatcaa
     1501 ttgggacgac ggtatcttat tttcaaaaca agccagccag ttgaaaacgg gtcagtgtcc
     1561 caactgcggg tcgaagcttg agcttgcagg caagggtgtg gtaaaatgcc cagcgtgtgg
     1621 tagtgaagtt tttctgagtt aacgttcaat atttaaaaag gagacgtatg ggcagcaatc
     1681 tcactgatca actcaagaac gatcgtggtg gtttttctaa actcctgagc cacatcccgg
     1741 gctttaaagg ttacatggaa aaagaaacgc gccgtgatgc ggataaatta atccgcgaca
     1801 cactcgtcac gcgtttcgat caacagctta gtcggctcac tggtgtgcag aatgatttga
     1861 tcgataacgg cgggcttgac tatgtggacg atgtcgaagg tgcagcggtt aaactgcgta
     1921 cctttatcgc tcgtgtcaag actgcgccgc gcggttatgc cggcatgttc gacgctgtgc
     1981 atgtccgcga ggagcagctg gatctactgt acaacttcga cgcgcagctg ctaagcgaag
     2041 tcgatcatat gtcatcggcg atcgatgggg tgagcgcggc ggtgaatccc acgccagcgg
     2101 tgccggcggc catgccggtt gccgatagtt ctgctacaac cggtagtgcg gctgtccccg
     2161 tacagcccag tgtgccggct cccgtcatga ccggaccagc cgcaatcaaa ggtgctattt
     2221 cggcgctgca actggtctgc gccggcttaa acgatttata tgataaacgc gaacacgtga
     2281 tgctagggtc agtctaatcg atcgggttaa tcaaatcttc gacaagaaaa aattggagga
     2341 gttacatgcc acgcattatc gatgtagttg agtttaatga tgaatcggga accgaaatcg
     2401 tccatcgcat acctgaaaat ggatcaggcg atttccgctt tggctcgcaa gtgattgtcc
     2461 gcgagcagca aaacgcagtt ttcttccgcg acggcaagtc gttggatgtg tttaaggccg
     2521 gtcgccatac tattaacacg gctaacttgc cgattctgag cggcttgatc ggactggcga
     2581 cgagcggcaa gacaccgttc actgccgaag tttattacgt gagcatgcgc caattcctgg
     2641 atatgggctg gggcacgccc gagccgatcg ctttccgcga cgccgatctc ggcatggtgc
     2701 ggttgcgcgc caatggagtg tactcgtttg aaattaagga cccgtcgctg ttcgtcaata
     2761 aagtcgtcgg cgcgcgaggc atcttccaga cttcagatat cgaagatatg ttgcgtagca
     2821 tcatcattag caagttcatt gatctgctgg gcgaagcaaa agttcctttc cttgatctcg
     2881 gcacgaagta caacgagtta agtgccggtg ccaaagccaa gattagcgct gacttcgagg
     2941 cgatgggtat tcagttgatg tcctattacg tccgagcgat tacgccgacc gaagaaacgg
     3001 ccaaggcgct ggacgagcgc gcgagcatgg gcgcaatcgg cgacatgaac aagtacctgc
     3061 aattccaagc ggcccgcggc atccgagacg cag
//
LOCUS       DASRTJ010000113         8891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_44570,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000113 DASRTJ010000000
VERSION     DASRTJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44570"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFF70_02335"
     CDS             complement(<1..220)
                     /locus_tag="VFF70_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43562.1"
                     /translation="MHRPYLIDSDRSENIRLAQQHQRVYTARSFPPLDGCTLHPSGPP
                     GQQAIQLNRQGETMFRQTDRSIRAQRFSH"
     gene            404..586
                     /locus_tag="VFF70_02340"
     CDS             404..586
                     /locus_tag="VFF70_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZY43563.1"
                     /translation="MKKFFRGMFTNMGVVGEVMTLLWQRKRWWLIPLVTVLLVFGLLL
                     IFAAASGVAPFIYTLF"
     gene            761..991
                     /locus_tag="VFF70_02345"
     CDS             761..991
                     /locus_tag="VFF70_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43564.1"
                     /translation="MNILKKFWAGWKRFGHLMGDLLARVVLTIFYFTIFLPFGLLTSL
                     FGDPLNIRSKAKPAWLKRETGDRSLVEARRQF"
     gene            995..2797
                     /locus_tag="VFF70_02350"
     CDS             995..2797
                     /locus_tag="VFF70_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZY43565.1"
                     /translation="MNILGVSCYYHDAAAALLQDGMLIAAAEEERFSRVKHDSNFPKH
                     SIEFCLKQGGITANNLDYVVFYEKPLVKFERILMTTLGQFPRSYPVFRESMITWFNEK
                     LWIKSQLQNDIGVPANRILFIEHHLSHAASALFASPFDEAAILTIDGVGEWTTATIGK
                     GTADWNRSDGEKRENKIDLIREVKFPHSLGLLYSAFTAWLGFQVNEGEYKVMGMAPYG
                     TPKYVDDVYKLVEVNDDGSFKLNMDYFSFHYSTHSTYNRKFSDLFGESRRPESEFFTT
                     ITNPKKDHPHWSDAAAAVNQHYADVAASIQHVTEEMILKMAGYAHQETGSQNLVMAGG
                     VALNSVANGRVIKETPFENVFIQPAAGDSGGALGAALYAYHVLLGKPRKFVMEHAYWG
                     QEYSVDEMRSAIEAKGFAYDLIEDDEKLLDQVVDRLTHAKVVSWFRGRFEWGPRALGH
                     RSILADPRSIEMKEVVNTKIKFREPFRPFAPVILEERTTEYFDIPTPEKYYPARYMLL
                     VTSIPENKRDKIQAVCHVDGSGRLQTVRREWNPSYYRVVEKFGEATGVPVLLNTSYNL
                     RGEPIVTTPQNALYTFGKSDIDALVMGQFLVKKN"
     gene            2960..4270
                     /locus_tag="VFF70_02355"
     CDS             2960..4270
                     /locus_tag="VFF70_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43566.1"
                     /translation="MVDTASQSITKSNPSMRRRIFSVIALVSITLLSVSVVLEIGLRI
                     FWRLIPLGVCASSPIIGNYYCQPYIVYDKPMQLAYHYAPGFKTEGYWDPADPHSANPG
                     NETAPTGRTDKFLYKMQMDSMGFPNDDVTWKDQYDIVIAGDSFVLRSAPQTWIELLRG
                     DTGMTTLALGAPSWAPINEVNAIETYGLDKHPKWVVLMYFEGNDLYGTGQFLERQSTG
                     LSWAEWDFQHSALGDQLIVPHLISYAVQQIFPSSATPHYRYPITASTEAGNIQMVLKD
                     FQLLALSADYNTLAHSDEFLAEKAKILEIRDLVEGQGGRFLLVYIPSKEHVYWSRIWD
                     SKDVNNILERTETVTLSDGDHGHLVYSPQYLSFDVFNQNQTAQETLFDDMTKQNNVEF
                     LNLMPVFWNATIQHGEFYNYADPHWNQAGNQLAADTIAEYIRTH"
     gene            complement(4352..5533)
                     /locus_tag="VFF70_02360"
     CDS             complement(4352..5533)
                     /locus_tag="VFF70_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43567.1"
                     /translation="MSLRKYGIALIAIALAACASPDRSPTPDRTSTPIETTPTLRPAP
                     SIAPSPTPTPVPKPDIGVQGQFIGPEGDKGVEQAANLGVGWIKQQIDWNSIEYARGLY
                     RWEEIDHLVMKAQQNHLQIMFSVARAPGFSRPEPIEEDGPPIDFTIFRDFMYAISSRY
                     TGRVAAYELWNEPNLAREWRGLDLSAEKFVELIKEGSQGVREGDPDAIIISGAPAVTG
                     IDDGVNAIDDRKFLKEMIDTGVGDLVDAIGAHPYGAANPPDERTADPIHLRSSYNTHP
                     SFFFLDTIEDYNQILSSANITQPIWITEIGWPSIEKFDNVDTTGWEYARDVTEADQAA
                     YLLRAIELRRDRTWLGPMIVWNLNIAPLLGSDRSESAYGLIRPDGSFRPAYKQLRNSG
                     E"
     gene            complement(5600..6283)
                     /locus_tag="VFF70_02365"
     CDS             complement(5600..6283)
                     /locus_tag="VFF70_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43568.1"
                     /translation="MPQTILAVDDDPAQIRLIQMTLMVVNYHVLSASNGEDGVHLFEV
                     QRPDLVVLDVMMPVMDGWEVCSRIRQTSTVPIIFLTARQTTDDKVSGLKIGADDYLIK
                     PFHPDDLLARVEAVLRRTYGPRQPLSDLLPAGPDILINRERREVFVRGDLQSLRPTEF
                     ALLVILAERAGVPMSASDIATALNIGQEHSAERVKWHVWKLRKSIEIDPDRPSIILTE
                     TGQGYRLVN"
     gene            complement(6318..7010)
                     /locus_tag="VFF70_02370"
     CDS             complement(6318..7010)
                     /locus_tag="VFF70_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43569.1"
                     /translation="MGETILVIEDDSFQAEVVEQLLAHNGFTPIIAMDGVEGLRRLYE
                     TQPDLVVLDLMLPHIDGWEVLRRIREMSTVPIIIMSSRKSDEEKIKGLRLGADDYIAK
                     PFNVQELAARIVAVLRRTHMPPPPKGSVLRFAGGNLIIDPESAAVIIDGQPRELTPTE
                     HQILMFMAQRPGQIISVEEIFSTIWGFESKANINNVKWYVWRLRQKMEANDKTPRFIF
                     TERGMGYRFTTV"
     gene            complement(7057..7257)
                     /locus_tag="VFF70_02375"
     CDS             complement(7057..7257)
                     /locus_tag="VFF70_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43570.1"
                     /translation="MFDDLRQQSAAPTEEEDEMLHESLIERMPIFKQLTPQQRFFIAL
                     LLLFNVVVLGCGCLLASGRVVP"
     gene            complement(7342..7983)
                     /locus_tag="VFF70_02380"
     CDS             complement(7342..7983)
                     /locus_tag="VFF70_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43571.1"
                     /translation="MNDTPVAKRMWRITLIIIIMSVAIPLSTAIIIATRATPYSDQII
                     WRGFDDHWSINSGSIAGDQWQVQLITNSIGLALHPIDYPAFTFQARISPSDPNLATGL
                     IINAQDTHNFTAFLISSDGYISLREKRSGQWIDRKPWGVWPHVRRDGQSNMLRVECDP
                     DRCIFFVNEEFTLQENIAASSTNLGLLAYPIGFSNQPAVVIFDQLSAARSFVP"
     gene            8023..>8891
                     /locus_tag="VFF70_02385"
     CDS             8023..>8891
                     /locus_tag="VFF70_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZY43572.1"
                     /translation="MFGRLLKILFVPCILLIFAVWQSGIVAAPVDDPIVLPVVTSTRK
                     PTPTPRPTAMPTLPPPATATLVPVATSTPLPSITPTPTKVIIVHPASDGVTRSVNVPI
                     LMYHYISAPPSPTDSLRVGLSVPPEKFDAQMNLLAERGYHSLTLLEMYTFLQNGEALP
                     TNPVVITFDDGYIDNYEFAFPILKKYGFIGTFFILSGPIDNGNPNYMTWDMVKELSDS
                     GMDIELHSRDHFDLRNRSNDFLVYQLVGGRQTIEAHTGQPIHFMAYPSGKYDAAVLRV
                     LASADFWGAVTTQ"
BASE COUNT         2064 a         2259 c         2415 g         2153 t
ORIGIN      
        1 catggctaaa acgttgggcg cggatggatc gatcggtttg gcggaacatg gtttctcctt
       61 ggcggtttag ttgaatggct tgctgtccgg gcggacccga cgggtggagc gtgcatccat
      121 cgagcggggg gaagctgcgc gcagtataca cccgttgatg ttgttgtgcg agacgaatat
      181 tctccgatcg atccgaatcg atgagataag ggcgatgcat tcccaatcga tcgatgttga
      241 tttgcgccca aatgcgatcg agatgttgat cgaaaacgat tcacgtggaa tgcatcgccc
      301 ctacgattat tattccacct tcttttttat gctacgtgcc gcttggttga caaagcgaca
      361 tgcccgcgtt agaatccgcg cagtaatcaa caggagtgat ggtgtgaaga agttctttcg
      421 cggcatgttt acgaatatgg gcgtcgtcgg cgaggtgatg acgttgttgt ggcagcgcaa
      481 gcgttggtgg ttgataccgc ttgtcacggt tttactggtg tttggtctgt tgttgatctt
      541 tgcagcggcg tcaggcgtag cgccattcat ctatacgttg ttctaaacta aaatgcggta
      601 tctaatgtca ggcagcgtca tggcggctga gcgagtgtgg tcgttgtgac gctgttttgt
      661 gtccgctgac acttgcaccg cactgcgtgc tatgcagtgc aggtgtgctc acagataaat
      721 cagagtgcct tggagcgcat ctgtagataa tggagaggat atgaatatct tgaaaaaatt
      781 ctgggcgggc tggaaacgct tcggccacct catgggcgat ctactggcgc gggtggtgtt
      841 gaccattttc tattttacga tcttcctgcc gtttgggttg ctgaccagtt tattcggcga
      901 tcccttgaat atccgatcga aagcgaaacc ggcctggctc aagcgcgaga cgggcgatcg
      961 atcattggtc gaagcgcgga ggcaattcta aggcatgaat attcttggtg tctcttgcta
     1021 ttatcatgat gccgccgcgg cgctgctgca agacggaatg ttgattgccg ccgcggaaga
     1081 agagcgcttc agccgcgtca agcacgacag caatttcccc aaacactcga tcgagttttg
     1141 tttgaagcaa ggtggcatca ccgccaacaa tctcgattac gttgtcttct atgaaaagcc
     1201 gctggtcaag ttcgaacgca tcttgatgac gacgctcggc caattccctc gatcgtatcc
     1261 ggtcttccgc gaatcgatga tcacttggtt caatgagaaa ctgtggatca aaagccaact
     1321 tcaaaacgat atcggtgttc cggctaacag gatactgttt atcgagcatc acttgtcgca
     1381 tgcggcgagt gcgctgttcg cctcaccttt cgatgaagcc gcgatcttga cgatcgatgg
     1441 cgtcggcgaa tggaccaccg ccacaatcgg gaagggcaca gccgactgga accgatcgga
     1501 cggtgaaaaa cgcgagaaca agatcgatct gatccgcgaa gtaaagttcc cgcattcatt
     1561 aggcttgttg tattctgcat tcaccgcgtg gcttggcttt caagtgaatg aaggcgaata
     1621 taaagtcatg ggcatggcgc cgtatggcac gcccaagtac gttgatgatg tttacaaatt
     1681 ggtcgaggtc aatgacgacg gcagtttcaa gttgaacatg gattatttca gtttccatta
     1741 ttcaacgcac agcacgtaca atcgaaagtt cagcgatctg tttggcgaat cgcgccggcc
     1801 cgaatcggaa ttctttacga cgatcaccaa tcccaaaaaa gatcatccgc attggtcaga
     1861 tgcggccgca gcggtgaatc aacattacgc cgatgtggcg gccagtattc aacacgtcac
     1921 cgaagagatg atcttgaaga tggcaggcta tgcccatcaa gaaacgggca gccagaattt
     1981 ggtgatggcc ggcggtgtgg cgttgaacag cgtggcgaat ggccgcgtca tcaaagagac
     2041 gccgtttgaa aatgtcttca ttcaaccggc cgcgggcgat tcgggcggcg cgcttggcgc
     2101 ggcattgtat gcttatcacg tgctgttagg caagccgcgc aagtttgtca tggaacacgc
     2161 gtattggggg caggaatatt cggtcgacga gatgcgctcg gcgatcgagg cgaagggctt
     2221 tgcgtacgat ttgatcgaag acgacgagaa gctgctcgat caagtggtcg atcgattgac
     2281 gcatgcgaaa gtggtgagtt ggttccgtgg ccgattcgag tggggacccc gcgcgctcgg
     2341 ccaccgatcg atcttagctg accctcgatc gatcgagatg aaggaagtgg tcaataccaa
     2401 gatcaaattc cgggaaccgt tccgcccgtt cgcgccggtg atcttggaag aacgcacgac
     2461 ggaatacttc gatattccga caccggaaaa atattatccg gcacgctata tgctgctcgt
     2521 tacctcgatt ccggaaaaca agcgcgacaa gattcaagcg gtgtgtcatg tcgacggctc
     2581 gggacgtttg cagacggtgc gccgcgaatg gaatccgtcg tattatcgtg tcgtcgaaaa
     2641 attcggtgaa gcgaccggcg tgccggtgct gttgaacacc tcgtacaatt tgcgcggcga
     2701 acctatcgtt acgacaccgc agaatgcatt gtacactttt ggcaagtcgg atatcgatgc
     2761 gttggtgatg ggtcagttct tggtcaagaa aaattgaccc tcgaaagaac ctgaaaaaca
     2821 atttgtgggg attcgttagc tactgagcta tcattcccgc gaaggcggga atccagtgcg
     2881 cgccgaatgg acgcccgcct tcgcggacgt gacaacgtcg gaggcagagc agataaaatt
     2941 agtggctgat agttttatta tggtagatac agcatctcaa tcgatcacca agtctaatcc
     3001 atcgatgcgg cgcagaatct ttagcgtgat cgcgctggtc tcgatcacgc tgcttagcgt
     3061 cagcgtcgtc ctcgaaattg gcctgcgcat tttttggcgg ctgattccac tcggcgtgtg
     3121 tgcctccagc ccgatcatcg gcaattatta ctgccaacct tatatcgtct acgacaagcc
     3181 aatgcagtta gcgtatcatt acgcgcccgg ttttaaaacc gaaggctatt gggatccggc
     3241 cgatccgcac agcgctaatc ctggaaacga gactgcacct actggacgca ctgataagtt
     3301 tttgtacaag atgcaaatgg attcgatggg ttttccgaac gacgacgtca cgtggaaaga
     3361 tcaatatgat atcgtgattg cgggtgattc attcgtgctg cgcagtgcgc cacagacgtg
     3421 gatcgagtta ctgcggggcg atacgggcat gaccacgttg gcgctcggcg cgcctagctg
     3481 ggcaccgatc aatgaagtca acgcgatcga gacgtatgga ttggacaaac atccaaagtg
     3541 ggtagtctta atgtacttcg aaggaaatga tttgtatggt acgggtcaat tcctcgaacg
     3601 acaatcgacg ggcttaagtt gggccgaatg ggatttccaa cactcagcgt tgggtgatca
     3661 attgatcgtg ccgcatttga tttcatatgc ggttcaacag atctttcctt cgagcgccac
     3721 gcctcattat cgctatccga ttacggccag cactgaagcg ggcaatatcc agatggtgtt
     3781 gaaggatttt caattactgg cgctgagtgc cgattacaat acgctggctc attcggatga
     3841 attcttggct gaaaaagcga agatactcga aatcagggac cttgtcgagg ggcagggcgg
     3901 gcgcttcttg ttggtctaca tccccagcaa agagcatgtg tattggtcgc gcatctggga
     3961 ttcgaaagat gtcaacaaca ttttggagcg gactgaaacc gtgacactca gcgacggcga
     4021 tcacggtcac ttggtatatt cgccgcagta tctcagcttt gatgtcttca atcagaatca
     4081 gactgcgcaa gaaacgttgt tcgatgatat gactaagcag aacaacgtcg agtttctgaa
     4141 tttgatgccg gtcttctgga atgcgaccat tcagcacggt gaattttaca actacgccga
     4201 tccgcactgg aaccaagccg gtaatcaact ggccgcggat acgattgctg agtatattcg
     4261 gacacattag ttccgatggt tgtcacaccc gcgaaagcgg gaatgaccga agtgattaag
     4321 agtccgcgtt tcaaaacgcg gcctcgtttt gttattcgcc tgaatttctc agctgcttat
     4381 aggctggtct aaaagatcca tccggtctga tcagcccata ggccgattcg gatcgatcgg
     4441 agccgagcag cggtgcgatg ttgagattcc agacgatcat cggccccagc cacgttcgat
     4501 cgcggcgcaa ttcaatcgcg cgcaacaggt aagcggcctg atcggcttcg gtcacatcgc
     4561 gcgcatactc ccagcccgta gtatcgacat tgtcaaactt ttcgatcgat ggccaaccga
     4621 tttccgtgat ccagatcggc tgggtgatgt tcgcgctgct caggatttgg ttatagtcct
     4681 cgatcgtgtc taagaagaag aaactgggat gggtgttgta actacttcgc aagtgtatag
     4741 gatcagcagt ccgttcgtca ggcggattgg ccgcgccata cggatgcgcg ccgatcgcat
     4801 cgacgagatc gccaacaccc gtgtcgatca tctccttcaa aaattttcga tcatcgatcg
     4861 cattgacacc atcatcgatc ccggtaacgg ctggcgcgcc gctgataatg atcgcatcag
     4921 gatcaccttc gcgcacgcct tgcgatcctt ctttgatcaa ctccacgaat ttttctgcgc
     4981 tgagatcgag gccacgccat tcgcgggcga ggttcggctc attccacaat tcatacgcag
     5041 ccactcggcc ggtatagcga gatgagattg catacatgaa gtcgcggaag atcgtgaagt
     5101 cgatcggcgg gccatcttct tcgatcggtt cgggtcggct gaagcctggc gcgcgcgcaa
     5161 cgctaaacat gatctgcaaa tgattctgct gcgctttcat cacgaggtga tcgatctcct
     5221 cccatcgata taagccgcgt gcatactcga tcgagttcca atcaatttgt tgtttgatcc
     5281 aaccaacacc gaggttcgca gcttgctcaa cgcctttgtc gccctctggt ccgatgaatt
     5341 gtccttgaac tccgatatcg ggtttgggca cgggcgttgg agttggcgaa ggtgcgatcg
     5401 agggtgcagg tcttagcgta ggggttgttt caatcggtgt ggatgttcga tcgggcgtag
     5461 gagatcgatc gggtgacgcg caagccgcca gcgcgatcgc gatcaacgcg atcccgtatt
     5521 ttcgaaggga catgcgggcc atcgtaccac atcaaaccaa aacccttggc cgcactcgcg
     5581 cgggttgccg gtcaaccgat caattgacta agcgatagcc ctggcctgtt tccgtaagaa
     5641 tgatcgacgg gcgatctgga tcaatctcga tcgattttct caacttccag acatgccact
     5701 tcactcgctc ggcgctgtgc tcctgaccga tgttcagtgc agtggcaatg tcactggcgc
     5761 tcatcggcac accggcgcgt tcggctaaaa tgaccagcag tgcaaactct gttggcctca
     5821 gcgattgaag atcgccccgc acaaacactt cgcgccgttc acgattgatc agaatatccg
     5881 gtccagccgg cagtaaatct gacaagggtt gacgcggtcc gtacgttcgc cgcagtaccg
     5941 cttcgacccg cgccaataga tcatcggggt gaaacggctt gatgagataa tcgtccgcac
     6001 cgatctttag gccgctgact ttatcatcgg tggtttggcg ggccgtcaga aaaataatcg
     6061 gcacagtgga agtctggcgg atgcgagagc agacttccca gccgtccatg acgggcatca
     6121 tcacatcgag cacgaccaga tccggccgtt gaacttcaaa caggtgtaca ccgtcttcgc
     6181 catttgacgc cgacaagacg tggtaattta ccaccatcag cgtcatctgg atgagtctga
     6241 tttgggcggg atcatcatcg actgctaaga tagtttgagg catatggcga gatacagctg
     6301 gcaaagccac tgagagtcta caccgtggta aagcgatagc ccatgccgcg ttcggtgaaa
     6361 atgaatcgag gagttttgtc gttggcttcc attttttggc gcagacgcca cacataccat
     6421 ttgacgttgt tgatgttcgc cttactctcg aagccccaga tcgtggagaa aatttcttca
     6481 accgaaataa tctggcccgg ccgctgcgcc atgaacatca aaatctgatg ctcggtgggt
     6541 gtgagttccc gcggttgacc atcgatgatc acagccgcac tctctggatc aatgatgaga
     6601 ttacctccgg caaagcgcaa gacactgcct ttaggaggtg gcggcatgtg cgtacggcgc
     6661 agcaccgcga cgatgcgcgc ggctaattcc tgtacattga acggcttggc gatgtaatca
     6721 tcggcaccta agcgcaggcc tttgatcttt tcttcgtcgc ttttgcgcga agacatgatg
     6781 ataatcggca ctgtggacat ttcacgaata cgacggagga cctcccagcc gtctatatgc
     6841 ggcagcatca ggtccagcac caccaaatcc ggctgcgtct catataagcg acgcagtccc
     6901 tccaccccgt ccatagcgat gatcggcgta aaaccgttgt gcgccaagag ctgctcaacg
     6961 acctcggctt gaaatgaatc atcttcaata acgagaattg tttcgcccat ttttaataaa
     7021 agtgcgtaat catatctgca acagcgccgg cgcagctcaa gggactactc gtccggaagc
     7081 cagcaggcag ccgcagccga gcacgacgac gttgaacaat aacaacagcg caatgaagaa
     7141 acgctgttgg ggcgtcagct gcttaaagat aggcatgcgc tcgatgagcg attcatgcag
     7201 catctcatcc tcttcttcgg ttggtgcggc cgattgttgc cggagatcat caaacattat
     7261 gcctcccagt tgcgtcgcta tgctttgtat cctaacgaag aatacctctg ccaattacaa
     7321 ttgtcaaatg actggccagg cctatggaac gaatgatcgg gcagcgctga gctgatcgaa
     7381 gatcacgaca gccggttgat tgctaaaccc gatcggataa gccaacaagc caaggttggt
     7441 acttgatgct gcaatgtttt cttgcaatgt aaattcctca ttgacaaaga atatacatcg
     7501 atcgggatcg cactctacgc gcagcatgtt cgattggccg tcacgcctga catgcggcca
     7561 gacgccccac ggcttccgat cgatccactg gcccgatcgt ttttcgcgca gactgatata
     7621 gccgtcgctg ctgatcaaaa acgcagtgaa gttatgagta tcttgtgcgt tgatgattaa
     7681 cccggtggcc agattgggat cgctgggact tatgcgcgct tgaaacgtga aggccggata
     7741 atcgatcgga tggagcgcta aaccgatcga gttggtgatc agctgcactt gccattgatc
     7801 gccggcgatc gatccggaat tgatcgacca gtgatcgtcg aagccgcgcc atatgatttg
     7861 atcggaataa ggagtggcgc gtgtggcgat gatgatcgcc gttgacagcg ggatcgccac
     7921 gctcataatt atgatgatca gggtgatacg ccacatgcgt ttggcgacgg gtgtatcgtt
     7981 catatgcttg acacggtgtg caggctaagg taagctgcgc ggatgtttgg tagattactg
     8041 aagattctat ttgtgccgtg cattctgctg atcttcgcag tttggcaatc cggcattgtt
     8101 gccgcaccgg tcgatgatcc gatcgtgctg cctgtcgtca cttcaacgcg caagcctacc
     8161 ccaactccga gacccacagc catgccgacc ttgccgccgc ctgcaacggc gaccttagtg
     8221 cctgtcgcaa cctcgacacc acttccatcg atcacaccga cgccaacaaa agtgattatt
     8281 gtgcatccgg cttcggatgg tgtaacgcgg agtgtgaatg tgcccatcct catgtaccat
     8341 tatatctccg cgccaccttc gcccacggat tcgctgcggg tgggcttgtc cgtgccgccg
     8401 gaaaaatttg acgcacagat gaatctgtta gccgagcgag gttaccatag ccttacactt
     8461 ctggagatgt ataccttctt gcagaacggt gaagccttgc cgactaatcc ggtggtcatc
     8521 acgttcgatg atggttacat tgataattat gaatttgcgt ttccgatctt aaaaaaatac
     8581 ggtttcatcg gcacgttctt tattttgtcc ggcccgatcg ataacggcaa tccgaattac
     8641 atgacctggg acatggtcaa agaactcagt gacagcggca tggatattga actgcacagc
     8701 cgcgatcatt tcgatctgcg caatcgctcc aatgatttct tagtgtatca acttgtcggt
     8761 ggccgtcaaa cgatcgaagc gcataccggc cagccaatcc atttcatggc ctatccttcc
     8821 ggcaaatatg atgcggctgt gttgcgcgtt ttagcgagcg cagatttttg gggcgcggtt
     8881 accactcaaa a
//
LOCUS       DASRTJ010000114         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82132,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000114 DASRTJ010000000
VERSION     DASRTJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82132"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFF70_02390"
     CDS             complement(<1..927)
                     /locus_tag="VFF70_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZY43573.1"
                     /translation="MPFKPVSTKVDFPAQEREVLLWWQESDAFNKLRQLRASSSKRYS
                     FLDGPITANNPMGVHHAWGRTYKDVWQRYYAMKGYSERWQNGFDCQGLWVEVNVEKDL
                     GFKSKRDIEAYGLAPFVILCKQRVLNYAAVQSEQSIRLGYWVNWDDPDQLRLLRDKLG
                     SNPNEVIEYQAPDGMIEGTVEQVVGRLGVGNAGGSYFTFSDENNYQIWGFLKKCFDQG
                     WIYKGHDVMPWCYRCGTGISQHEIVTEGYVDLIDPGLTVRFPLVGHWGDGETKESLLV
                     WTTTPWTLTSNVAAAVGPDMTYVKVKQGDEYFY"
     gene            complement(958..>1567)
                     /locus_tag="VFF70_02395"
     CDS             complement(958..>1567)
                     /locus_tag="VFF70_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZY43574.1"
                     /translation="LYSGGGGQPYDLGTLSIGDQKWNVVKSSKQGSAIWHELDREPPA
                     VGSKVHGSIDWERRYQLMRTHTAMHILCGVIFRDYGASVTGGNMEPLKGRMDFEFETM
                     RQEFVKEIEVKINAEIANARPTRMKILPRDEAFQIPDLIRTKIDLLPEGITEIRTVEI
                     VGLDLQADGGTHVANTSEVGHIKIVDYKSKGKINKRIEVEIK"
BASE COUNT          353 a          465 c          358 g          391 t
ORIGIN      
        1 gtaaaagtat tcgtcgcctt gcttgacttt cacatacgtc atatcggggc caactgcggc
       61 cgccacgttc gaagtcaatg tccaaggagt cgtcgtccag accagcagcg actctttcgt
      121 ttcaccatcg ccccaatgac caacgagtgg gaatcggacg gtgagcccgg gatcgatcag
      181 gtcgacatag ccttcagtca caatttcgtg ctggctgatg ccggtgccgc agcgatagca
      241 ccatggcatg acgtcatgac ccttgtagat ccagccttga tcgaaacact ttttcaagaa
      301 gccccaaatt tgataattgt tctcgtctga aaaagtgaag tacgatccgc cggcgttacc
      361 gacacccaat cggcctacca cttgctcgac cgtgccttcg atcattccat cgggcgcttg
      421 gtattcgatc acttcgttcg ggttcgatcc caatttatcg cgcagcaaac gcagctgatc
      481 tggatcatcc caattgaccc agtaacccaa ccggatcgat tgttcggatt gcactgcggc
      541 gtaattcagc acgcgctgct tgcacaaaat cacaaagggc gcgaggccgt atgcttcgat
      601 atcgcgcttg ctcttgaacc ctaaatcttt ttcaacattc acctcaaccc acaaaccttg
      661 gcagtcaaag ccgttctgcc agcgctcgct gtagcccttc atcgcgtagt agcgctgcca
      721 aacatctttg tacgtgcgcc cccaggcgtg atgcaccccc atcggattgt tggccgtgat
      781 cgggccatcg agaaacgaat aacgcttcga cgacgatgca cgaagttggc gcaatttgtt
      841 gaacgcatcc gattcttgcc accacaacag cacctcgcgt tcttgcgccg gaaaatcgac
      901 tttggttgaa actggtttaa aaggcatgac acttctcctg aatttcgttt gatccactta
      961 cttaatctca acttcaatcc gcttattgat cttgcccttg ctcttgtaat caacaatctt
     1021 aatatgaccc acttcgctcg tgttggcgac atgcgtgccg ccatcggcct gtagatcgag
     1081 gccgacgatc tcgaccgtgc ggatttcggt gatgccttcc ggcagtaaat cgatcttcgt
     1141 ccgaattaga tcgggaatct gaaaagcctc atcgcgcggc aaaattttca tgcgcgtcgg
     1201 tcgagcattc gcaatttcgg cattgatctt cacttcgatc tctttgacga attcctgccg
     1261 catcgtctca aattcaaaat ccatgcgacc tttaagtggc tccatgttac cgccggtgac
     1321 cgatgcaccg tagtctcgaa agatcacacc acacaaaata tgcatcgcgg tgtgcgtgcg
     1381 catcaattga tagcgccgct cccaatcgat cgagccgtgc actttcgatc caacggctgg
     1441 cggctctcga tcgagttcgt gccagatggc cgaaccttgc ttgctgcttt tgacgacgtt
     1501 ccacttctga tcgccgatcg acagcgtgcc aagatcatac ggctgtccgc cgccacccga
     1561 atacaac
//
LOCUS       DASRTJ010000115         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_116367,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000115 DASRTJ010000000
VERSION     DASRTJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116367"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VFF70_02400"
     CDS             <1..1327
                     /locus_tag="VFF70_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43575.1"
                     /translation="LLSFAALLLLFSPLLALILFGATIPAFLAQSQYAQLNFRLLSWR
                     APEARRMNYLEHLLTVDSSVKEVKLFGLGEPLLGRYADLFWKFLKEDQALAQKRSFAS
                     FGWGLLATLSYYLSYGWIVLRTIDRSITLGDMSLYLSIFRNSQNIFQSLFFGLSDLYE
                     NSLFMSNLFSFLELEPLMPIAAHPIDLPRPIVRGIEFKNVSFQYADRPEYALKNVNLT
                     IRPGEKLALVGANGAGKTTLVKLLTRLYDPTEGQITIVGIDLREIDPKELQKRIGVIF
                     QDFVKYFLPARENIGFGQIDQLDDEERIIDASKRSGAHETLTALPQGYDTMLGKWFER
                     GNDLSGGEWQKVALGRAFMREAEVLVLDEPTAALDALNEYAIFQRFRELTDNKIALLI
                     SHRFSTVRMADRIIVLEHGEVTEMGTHQSLVDLGGMYAKLFNIQAEGYR"
     gene            1328..1867
                     /locus_tag="VFF70_02405"
     CDS             1328..1867
                     /locus_tag="VFF70_02405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZY43576.1"
                     /translation="MTNDKLPLTNESKQPERSGEAVGFNAKTTPGGPPTLRWRVGYGQ
                     DADIIESTFHVRYAETDQMGIVHHSAYVIWMEEGRSEFMRQKHVDYANVEKRGLSLAV
                     TELNVRYIASAHYGEQVTVRTWVDSLRSRMLVFGYEIVNAESKQKLIKGSVKLILIDQ
                     QGQVVTMPEEIQSALRPYT"
     gene            1912..>2455
                     /locus_tag="VFF70_02410"
     CDS             1912..>2455
                     /locus_tag="VFF70_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZY43577.1"
                     /translation="MKKADRNALITFPILILIGLLVALAGSQGGSTVAGIPVLALSVG
                     LAYLIQWLVFIPSYLRQTEKLFDLTGSLTYISISTIAVLLSPNIDGRSILLWLVVVIW
                     AIRLGSFLFGRIRKAGKDERFDEIKPSFIQFLNFWTIQALWITFTAAAALVAITATSR
                     KDLDVFAVIGVLVWVAGFAIE"
BASE COUNT          564 a          644 c          664 g          583 t
ORIGIN      
        1 cctgctctcc ttcgctgcct tactgctgct ttttagtccg ctgctcgcgt tgatattatt
       61 tggcgcaacg atcccggcct tcctggcgca atcgcaatac gcgcagttga attttcgctt
      121 gctgtcgtgg cgcgcgcctg aagcgcggcg catgaattat ctggaacatt tgttgaccgt
      181 cgattccagc gtgaaggaag taaaactctt cggcctgggc gaacccctgc tcggccgcta
      241 cgccgatctc ttctggaaat tcttgaaaga agatcaagcg cttgcgcaaa aacgcagttt
      301 cgcatcattc ggttggggct tgctcgctac gctctcctac tatctcagtt acggctggat
      361 cgtcctgcgt acgatcgatc gatcgatcac gctgggcgac atgagtttgt atctttcgat
      421 cttccgcaac agccaaaata ttttccagag tttgttcttc ggcttgagcg acttgtatga
      481 gaactcgttg ttcatgagca atctgttctc atttctcgaa ctcgaaccgc tgatgccgat
      541 cgcggcgcac ccgatcgatc tgccgcgtcc gatcgtgcgc gggatcgaat tcaagaacgt
      601 gtcgtttcag tatgccgatc ggccggagta cgccctgaag aacgtcaatt tgacgatcag
      661 accaggcgag aagctcgcct tagtcggagc gaatggcgca ggcaagacca cgctcgttaa
      721 actgctcact cgattgtatg atcccaccga aggacagatc acgatcgtcg ggatcgatct
      781 gcgcgagatc gatcccaaag aattgcagaa gcgcatcggt gttatctttc aagattttgt
      841 taagtatttc ttgcccgcgc gcgagaacat cggcttcggc cagatcgatc aactggacga
      901 tgaagaacgc atcatcgatg cgtcaaagcg atcgggcgcg catgaaacgc tcaccgccct
      961 gccgcaaggc tatgatacga tgctcggcaa atggttcgag cgaggcaacg atctatcggg
     1021 cggcgaatgg cagaaagtgg cgctgggccg cgcgttcatg cgcgaggctg aagtgctggt
     1081 gctcgatgag ccgacggccg cattggacgc cttaaacgag tacgcgatct ttcagcgctt
     1141 ccgcgaattg accgacaaca agatcgcgct gctgatcagt caccgcttct cgacggtgcg
     1201 catggctgat cggatcatcg tgctggagca cggcgaagtc accgagatgg gcacacatca
     1261 aagcttggtt gatctcggcg gcatgtatgc caagttgttc aacatccaag ccgaagggta
     1321 ccgataaatg accaatgaca aattaccact gacaaatgag agcaagcagc ctgagcggag
     1381 cggggaagcc gtaggcttca atgcgaagac gacgcctggc ggcccaccaa ctctccggtg
     1441 gagagttggg tatgggcagg acgcggacat catcgaatcc acctttcatg tccgctacgc
     1501 cgaaaccgat cagatgggca tcgtgcatca cagcgcgtac gtcatctgga tggaagaagg
     1561 ccgatcggaa ttcatgcggc agaagcacgt tgattacgcc aacgtagaaa agcggggact
     1621 gtcattagct gtaaccgaat tgaatgtgcg ctatatcgct tcggcacatt acggcgagca
     1681 agtcaccgtt cgcacgtggg tcgattcgct gcgcagccgc atgctcgtct tcggctacga
     1741 gatcgtcaac gctgaatcga aacaaaaact gatcaagggc agcgtgaagt taatcctgat
     1801 cgatcaacag ggccaagtgg tgacaatgcc ggaagagatt caatctgcat tgcgacctta
     1861 tacgtagtcc tctttataaa agagagagag aatactgaag gagcaatttc catgaagaaa
     1921 gccgatcgga atgcacttat cacttttcca attttgatcc tcattggtct cctcgtcgct
     1981 ttggcgggga gtcagggagg gagcacagtc gccggaatcc ccgtactggc cttgtcggta
     2041 gggctggctt acctcatcca gtggctggtc tttatcccat catatttgcg acagactgaa
     2101 aaactcttcg atcttacggg cagtctcacc tatatttcca tcagcaccat tgcagttctc
     2161 ttaagtccca acatcgatgg tcgatcaatt ctgctctggc ttgtggttgt gatctgggcc
     2221 atccgtctgg gctcattcct gttcggccgg atcagaaagg caggcaagga cgaacgcttc
     2281 gatgaaatca aaccgtcctt tatccaattc ctaaattttt ggactatcca agcgttatgg
     2341 atcacattta cagccgcggc cgcgttagtt gctattacgg cgactagccg aaaagatttg
     2401 gatgtgtttg ccgtcatcgg cgttctcgtg tgggtggcgg gctttgcgat agagg
//
LOCUS       DASRTJ010000116         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82170,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000116 DASRTJ010000000
VERSION     DASRTJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82170"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFF70_02415"
     CDS             <1..323
                     /locus_tag="VFF70_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY43578.1"
                     /translation="MLEVYGWNLLGDATGCSIPNAGNWGLISLNTIGQLQDNGGPTWT
                     YALLPDSEAIDTTLDSLGCVDETLALLTTDQRGWARPVGVRCDVGAFEYSPPHYVYLP
                     LILR"
     gene            708..1196
                     /locus_tag="VFF70_02420"
     CDS             708..1196
                     /locus_tag="VFF70_02420"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZY43579.1"
                     /translation="MNAEKSPNKSGTRLNEIVLSVSLNLIWILMALLAVWLGWRSYTL
                     STTGSVAEGTVVRLLEDDKAAFDSEFTPIVEFQADGKTYTVQSQNSYRWWDRYLRFWV
                     GGHVKMLYDPAQPERAEINSWLDIWLEPILLGIFSIIAALAINAYLRYRWRSRRATQI
                     AS"
     gene            complement(1370..1990)
                     /locus_tag="VFF70_02425"
     CDS             complement(1370..1990)
                     /locus_tag="VFF70_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZY43580.1"
                     /translation="MGSSADIPDIVIGRLPIYLRALSFMGITGQEITSSAELAEKLGI
                     SSAQIRKDLSHFGEFGKQGTGYRISHLQEQLKRILKVDREWAVALIGVGDLGHALARY
                     GGFKDRGFQIVLAFDSDPIKIGSEVGDLTVQDVAHLPEMIKKHNVQVAMLTVPAKDAQ
                     AAAEMCVKAGIKAILNYAPINLTLPDDIFVQYTDPVTHLQRMTYYL"
     gene            2256..3611
                     /locus_tag="VFF70_02430"
     CDS             2256..3611
                     /locus_tag="VFF70_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZY43581.1"
                     /translation="MLRRKWWIFAIIALVIIGIVLASRSQPAAPTAERTALVARGTIN
                     ASVSASGNIDPEAKVDLSFGTVGTVQEIVVSEGQSVKKDDVLARLDSGDLDLAVKQAE
                     QSLTIQQLVYSQTVAPSADDLAATQANLASAAANLKQLQGNPDPLQQDIARLQRDNAN
                     ETRYQVQLRWDQVSDKPVGGIGVDTLKSQYAQAVIGAQIAQLQYELILRGGNDAQTAA
                     ARAQVAQAQSALNKLKGDDRSRALALAQLQQSALNLDQAKLRLQKATLVAPIAGTVSE
                     LNVSVGESVGAGNLQPAVVVADLTSFHINVGVDESSVGQLKVGQPVRITIDALTDQPL
                     TGHVDRIAPTASNVGGIISYKVTITLEPTTALVRGGMSANVEIVTDTHENILIVPNWT
                     IRIDRATGKTYVNVRRDGKIQEVEILTGLRNASESEVTSGANEGDVLVVPQTSGLPFG
                     G"
     gene            3696..4406
                     /locus_tag="VFF70_02435"
     CDS             3696..4406
                     /locus_tag="VFF70_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43582.1"
                     /translation="MNDQPVIEINNIVKTYIMGETEVNALRGATLTVQTGELVSIMGP
                     SGSGKSTLMNIIGCLDQPTSGSYKLDGVDVHSLDDNALAEIRNRKIGFVFQQFNLLPR
                     TTALQNVELPLIYSGNNRQRRDKAKAALESVGLADRLSHRPSELSGGQQQRVAIARAL
                     VNDPSIILADEPTGNLDSKSGAEIVAIFQRLNRDKGITVVFVTHDPMIASHTKRIIRI
                     HDGQIVGDEPVTDQLIEM"
     gene            4458..>4837
                     /locus_tag="VFF70_02440"
     CDS             4458..>4837
                     /locus_tag="VFF70_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY43583.1"
                     /translation="MINTSTLSENIRMALRALIANKLRAILTMLGIIIGVSAVITLLS
                     VGRGVERFIGQAFGSIGTNLVFVLPGAPDATSSGPPSGPALLGNVALGPAVTMKDAEA
                     LRDQFRVPDIATIAPEISTSATAAV"
BASE COUNT         1172 a         1312 c         1312 g         1041 t
ORIGIN      
        1 ggatgctgga agtctatggc tggaacctcc taggcgatgc gacgggctgc agcatcccaa
       61 atgctggcaa ctggggactc atcagtctga acacgatcgg ccaattgcag gataacggcg
      121 ggccgacctg gacatacgcg ctcctgccgg atagcgaggc gatcgatacg acactcgaca
      181 gcctcggctg cgtggatgag acactggcgc tgttgacgac tgaccagcga ggctgggcgc
      241 gtccggtggg cgtgcgctgt gatgtcggcg cgttcgagta cagcccgcca cactacgtct
      301 acctgccgct gatcctgcgt taacgtcgta aacacaccac aaaagagcgc ccttgatcaa
      361 agacgctctt tttcgtagca acggaggtta cagcctgcgc caattcagcc gagccgcctg
      421 acgatgacca gatagttcat aaacacattg cccgccgact gtgtcgatcg attgccaacc
      481 aacacctcct caccgccact aacgcagacc gtttggcgct tgaacccatg cacaaatgaa
      541 caatggcaat ttacagcatg aggaagaatt cctccaaccg acagattgtg ttttccttat
      601 aaacgaaaga taatagcaag cctgctcttc aagccaaggg cagcagggga agtggttgtc
      661 attgaattga atggcaatca ttttcatcta acaaaaggag atgtctcatg aatgctgaaa
      721 agtcaccgaa taaatctggc acgaggttga atgaaatcgt tttgagtgtg tcccttaatc
      781 ttatttggat attgatggcg ctgctggccg tgtggctggg ttggcgcagt tacactttaa
      841 gcacaaccgg cagtgtggcc gaagggacgg tggtgcggct gctggaagat gacaaggcgg
      901 ctttcgactc cgaatttact cccattgtgg aattccaagc ggacggcaaa acgtacaccg
      961 tccaaagcca gaatagttat cgctggtggg accgctacct taggttctgg gtcggcgggc
     1021 acgtgaagat gctttacgac ccggcccagc ctgaacgtgc agagatcaat tcctggttgg
     1081 atatttggct ggaaccgatt ctgctgggca tattttccat catcgccgcg ctggctatca
     1141 atgcatacct gcgttaccgc tggcggagcc gacgtgctac ccaaattgca tcttgatcga
     1201 gcacagcagg cataattgaa ttgctcagcg cacaatccat ttttactcgc tctgcgcagt
     1261 caaagagcat tcaccaccac taacgcaaac cgttaggcac aatcgtaaac acacgaaaaa
     1321 gagcgccgtt gatcaagggc gctctttttc gttgcgaaga tcgatcgggt tacaagtaat
     1381 acgtcattct ttgcaaatgc gtgaccggat cggtatactg cacgaaaata tcatcgggca
     1441 gtgtgagatt gatcggcgca tagttgagga tcgctttgat gccggccttc acacacattt
     1501 cagcggcggc ttgcgcatct ttggcaggca cggtcaacat cgctacttgc acgttgtgct
     1561 tcttgatcat ctccggcaga tgagcgacat cttgaaccgt taagtcaccg acttcgctgc
     1621 cgatcttaat cggatcgctg tcaaaggcca gcacaatctg gaagccgcga tctttgaagc
     1681 cgccatagcg ggcaagcgcg tgaccgagat cgccgacacc gatcagcgcc acagcccact
     1741 cccgatcaac tttgagaatc cttttaagct gttcttgcaa gtggctgatg cgataaccgg
     1801 tgccctgctt gccaaactcg ccgaagtgcg acaaatcttt acggatctgc gctgagctga
     1861 tgcctaactt ctcagctaat tcggccgatg aggtgatctc ctgtccggtg atccccatga
     1921 acgatagcgc ccgcagataa atcggtaggc gtccaatgac aatatccgga atgtctgctg
     1981 aagatcccat gctgctccta aaaatccgtt cgtaaagatt tgaacaaccg atcgctatgt
     2041 taccacggaa caaagtcgcc cgcaaataat ttcttcacgt ttgattcatc ctttacacac
     2101 aagatgcaga atataattaa agcaggtata atctggcggc tgatgtagcc aatttgatat
     2161 tagaagttgg aggttggaag atttatcgct gaagcgaacc gaccctccag gctccaagat
     2221 ccaaattcca acttccataa taatgagagg tcaatatgct tcgtcgtaag tggtggatat
     2281 tcgcaatcat cgcacttgtt atcatcggga tcgtgctcgc cagccgatcg caacccgcag
     2341 ctcccaccgc tgagcggact gcgctcgttg cacgcgggac gatcaacgcc tctgtcagcg
     2401 ccagcggcaa catcgatccg gaagccaaag tcgatctgag ttttggcacg gtcggcactg
     2461 tgcaagagat tgtggtgagt gaaggccagt ccgttaaaaa ggatgatgtg ttagcccgat
     2521 tggatagcgg cgatctcgat ctcgcagtca agcaagccga gcaatctttg acgattcagc
     2581 agttggtgta cagccaaacg gttgctccgt ccgccgatga tctagcggca acccaggcga
     2641 acctcgccag cgcggccgcg aatctgaaac agctgcaagg caaccccgat ccgctgcaac
     2701 aagatattgc cagactgcaa cgggataatg cgaatgaaac gcgctatcaa gttcagctgc
     2761 gctgggatca agtcagcgac aagcccgttg gcggcatagg tgtcgatacg ctcaagtcgc
     2821 aatacgcgca ggccgttatc ggcgcgcaga tcgcgcagct gcaatatgaa ttgatcttgc
     2881 gcggcggcaa tgatgctcaa actgcggcgg cgcgcgcgca ggtggcccaa gctcaatcgg
     2941 ctttgaacaa actcaagggt gacgatcgat cgcgagcctt agccttggcg cagctgcaac
     3001 aatccgcgct gaatttggat caagccaagc tgcgcttaca gaaagccacg ctcgtcgcgc
     3061 cgatcgctgg cacggtatcg gagttgaacg tctcggtcgg cgagtcggtc ggcgcaggca
     3121 atttgcaacc ggctgttgtc gtagccgatc tgacttcctt ccacatcaac gtcggcgtcg
     3181 atgaaagcag cgtcggccag ttgaaagtag gccaacccgt gcgcatcacg atcgatgcgc
     3241 tgaccgatca acctctgacc ggccacgttg atcgcatcgc gcccaccgcc tcgaatgtgg
     3301 gcgggatcat ttcatataaa gtcactatca cgctcgagcc caccacagca ttagtgcgcg
     3361 gtggcatgag tgctaacgtc gagattgtga cggatacgca cgagaatata ttgatcgtcc
     3421 cgaactggac gatccgcatc gatcgagcga cgggcaagac atacgttaac gttcgacgcg
     3481 atggcaagat tcaagaagtg gaaattctca ccggcctgcg caacgcgagc gaaagcgaag
     3541 tgacttcggg cgcaaatgaa ggcgatgtgt tggtcgtgcc gcagacaagc ggtttgccgt
     3601 ttggcggcta agcgacgaac gaccaatgac gaacgacgaa aatattggtc attcgtcatt
     3661 ggtcgttcgt ctatcaaatt gaaaaattat tcagaatgaa cgatcaacct gttatcgaaa
     3721 tcaacaatat cgtcaagacc tatatcatgg gcgagacgga agtcaacgcg ctgcgcggcg
     3781 cgacactgac cgtgcagacc ggtgaactcg tatcgatcat gggcccaagc ggctcaggca
     3841 aatcgacgct gatgaacatc atcggttgtc tcgatcaacc cacgtcgggc tcgtacaaac
     3901 tcgatggggt agatgtgcat tcgctcgatg acaacgcttt ggccgagatc cgaaatcgca
     3961 agatcggttt tgtgtttcag caattcaatt tactgccccg cacaacagcc ttgcaaaatg
     4021 tcgaactgcc gttgatctat tcaggcaaca atcggcagcg gcgcgataaa gcaaaggccg
     4081 cgctcgaatc ggtcgggtta gccgatcggc tgagccaccg gcccagcgag ttgtccggcg
     4141 gacagcagca gcgcgtggcg atcgcgcggg cattggtcaa cgatccatcg atcatcttgg
     4201 ctgacgaacc gaccggcaat ctcgattcga aatcaggcgc ggagatcgtg gcgatcttcc
     4261 aacgcttgaa tcgcgacaaa ggcatcacgg tggttttcgt cacgcacgat ccgatgatcg
     4321 ctagccacac taagcgcatc attcgcattc acgacggaca gatcgtcggt gatgagccgg
     4381 taacagatca attgattgag atgtagggac gcacggcggt gcgtccaggg cgggcggctg
     4441 ctcgcctcta cgaaataatg ataaacacct ctactctttc tgaaaacatc cgcatggcgc
     4501 tgcgtgcgct catcgccaac aaactgcgcg cgatcttgac gatgctcggc atcatcatcg
     4561 gtgtctcagc agtgatcacg ctgctgtcgg tcggtcgtgg cgtcgaacga ttcatcggcc
     4621 aggcattcgg cagcatcggc acgaatttgg tgttcgtgct gcccggcgcg cctgatgcca
     4681 cgagttccgg tccgccgagc ggccccgcgt tgttaggcaa tgtggcgttg ggcccggccg
     4741 tgacaatgaa agatgctgaa gcattacgcg atcaatttcg cgtgcccgac attgcaacga
     4801 ttgcgccaga gatttcaacg tcggccaccg cggcggt
//
LOCUS       DASRTJ010000117         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_6788,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000117 DASRTJ010000000
VERSION     DASRTJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6788"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..337
                     /locus_tag="VFF70_02445"
     CDS             8..337
                     /locus_tag="VFF70_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZY43584.1"
                     /translation="MGDAVHEAMVETINVPANDRFQIFTAHQANELIADPTYLNVERS
                     GEVIFVQITLNLGRTVDQKQALYKRIAELVSTQAAIRPEDIVINLIELPKENWSFGNG
                     EAQYVQK"
     gene            366..>2254
                     /locus_tag="VFF70_02450"
     CDS             366..>2254
                     /locus_tag="VFF70_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43585.1"
                     /translation="MGMHGGGGWFSYIRSNDEKPRVTWTMFKRVLRYAQPYRRHIVGM
                     LVLILISTSVGLLRPLLVRDLIDHTIPAGDISRLLILALALLTIPAVQAVTGVAQRRL
                     NARVGEGVVYDLRVALFSHLQRMSLRFFTHTKIGELMSRLNNDVLGAQNAISNTVVNL
                     VTNVIQVIAVLAVMLALEWHLTLVSIIILPLFILVARRLAALLRNIAREQMEANAHMN
                     AMANETLSIGGALLVKLFGRTKVEVDRFSARARRVQDTGIHRAVVGSIFFAIMGVVSA
                     VGVAFIYSIGGYLVIQKAFTIGTIVAFGAYLSDLYGVLQGLANAPVDFSTSMVSFERV
                     FEVIDLPADIVEKSQAQALQAVNGQLSFEDISFRYEIQAANLLSDVRRFGQIDRVNAI
                     ISGSEQPVNGRSDPAGEDAVAHNQAREAALDHISFVVQPGQLVALVGPSGAGKTTLTY
                     LIPRLYDPTSGRILIDGHDLRDVTLDSLAEQIGMVTQETYLFHDTIRTNLLYARLDAV
                     QPEIEAAARAANIHDFIMNLPDRYDTIVGERGYRLSGGEKQRIALARVILKDPRVLVL
                     DEATSSLDSESEALIQEALKHVMAGRTSIVIAHRLSTILAADLILVMDRGQIVERGTH
                     AELLG"
BASE COUNT          489 a          541 c          663 g          561 t
ORIGIN      
        1 ccacgcgatc ggagatgcgg ttcatgaagc aatggtcgag acgatcaacg tgccggccaa
       61 cgatcgattt cagattttca cggcgcatca agccaatgag ctgatcgccg atccgacgta
      121 cttgaatgtt gagcgctcgg gtgaggtgat cttcgttcag attaccctca atttagggcg
      181 cactgtcgat cagaagcagg ctttatacaa acgcatcgct gagttagtct cgacgcaagc
      241 ggcgatccgg ccggaagaca ttgtgatcaa tttgatcgaa ttgccaaaag aaaattggtc
      301 atttggaaat ggtgaggctc agtatgttca gaagtaattt gaagaaatgg gtggggcaat
      361 aaattatggg gatgcatggt ggcggtggtt ggtttagcta tatccgatcg aacgatgaaa
      421 aacccagggt aacttggact atgtttaagc gcgtgctgcg ttatgcccag ccttatcgaa
      481 ggcatattgt gggcatgttg gtgttgatcc tgatcagcac cagcgttggt ttgctgcggc
      541 cgctgctcgt gcgcgatctg atcgaccata ccattccggc cggcgatatt agccgcttgc
      601 tgattttggc gctggctttg ctcacgatcc cggccgtcca ggctgtgacc ggtgtggcgc
      661 agcggcggtt gaatgcgcgc gtgggtgagg gggtggtcta cgatctccgc gttgcattgt
      721 tcagccactt gcagcgcatg tcgctgcgct tttttacaca cactaaaatt ggcgagttga
      781 tgagccgcct gaacaatgat gtgttgggcg cccagaatgc catcagcaat accgtcgtca
      841 atcttgtgac gaatgtgatt caggtcatcg ccgtattagc ggtgatgctg gccttggaat
      901 ggcatctaac gctggtgagt atcattatct tgccgctgtt tattctggtc gctcgccgct
      961 tagcagcact gctgcgtaac attgcgcgcg agcagatgga agcgaatgcg cacatgaatg
     1021 cgatggcgaa tgagacgctc agcattggcg gcgcactgct ggttaaattg ttcggtcgaa
     1081 caaaagtgga agtcgatcga ttttctgcgc gggcacgacg cgttcaagac acgggcattc
     1141 accgcgcagt cgtgggcagc atctttttcg ccatcatggg tgtcgtcagt gcagtggggg
     1201 tggctttcat ctacagtatc ggcggctatc tggttattca gaaagcattc accattggca
     1261 ccatcgtggc ttttggcgca tacctcagtg atttgtatgg cgtgttgcag ggcttggcca
     1321 acgcgccggt cgatttctcc acatcgatgg tcagctttga gcgtgtcttt gaagtgatcg
     1381 atctccccgc ggacattgtg gaaaagtctc aggcgcaggc cttgcaggcg gtcaacggcc
     1441 aattgagctt tgaggatatt tctttccggt atgaaatcca agcggctaac ttgctgagtg
     1501 atgtgcgccg tttcggacaa atagatcgag tgaacgcgat tatatccggt agcgagcaac
     1561 cggtaaacgg acgcagcgac ccagccggtg aagatgccgt tgcacacaat caagcgcgtg
     1621 aagcggcgct cgatcatatt tcgttcgtgg tgcagcctgg tcaactggtg gctttagtcg
     1681 gtccgagcgg ggcgggcaag acgactttga cctatctaat tccccgattg tatgatccaa
     1741 cgtcagggcg catcctgatc gatggacatg atctgcgcga tgtaacgctc gattcattag
     1801 cggagcagat cggtatggtg acgcaagaga cctatctctt tcacgatacg attcgcacca
     1861 atctcctcta tgcgcgtttg gatgcggtgc agcccgagat cgaagcggca gcacgtgcag
     1921 cgaacatcca tgatttcatc atgaacctgc ccgatcgcta cgacacaatc gtcggggaac
     1981 gcggctatcg attaagtggc ggcgaaaagc agcgcatcgc tttggcgcgg gtgatcttaa
     2041 aagatccgcg cgtgctcgtt ttagatgaag ctaccagttc gctcgacagc gaatccgaag
     2101 ccttgattca ggaagcgttg aagcacgtga tggccgggcg gaccagtatt gttattgcgc
     2161 atcgcttaag cacaattctc gccgccgatc tgatcttggt gatggatcgc ggtcagattg
     2221 ttgagcgtgg tacccatgct gaattgttag ggca
//
LOCUS       DASRTJ010000118         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_116442,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000118 DASRTJ010000000
VERSION     DASRTJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116442"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..656)
                     /locus_tag="VFF70_02455"
     CDS             complement(54..656)
                     /locus_tag="VFF70_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43586.1"
                     /translation="MNLVEIGTIVRLQIQRKGLKVGQKPYRIYDPSPILSVDRLRVTP
                     KGAQALLLNDDTAIDVHHVDHPDSKNAGDNSISIGFAANYKKMRDRFGDHMFIGCAGE
                     NILIETDRPIGPGVLSRGLAFRLGSSDDYLWLGQIKVALPCVEFSRYSSQMPQAERDT
                     AVIKETLQFLDGGLRGYYTLPTNSDQPLVVSVGDRVYLPE"
     gene            complement(764..1714)
                     /locus_tag="VFF70_02460"
     CDS             complement(764..1714)
                     /locus_tag="VFF70_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY43587.1"
                     /translation="MNSRSSSFGPTDLLLLMTIGLWAINFTVVKTIISGDLPPIAFTA
                     LRFTLATIGMLLVMRSFRDEQPLTRRDVLKMAGLGLLGNGLYQVMFIIGIDNTSLTNS
                     ALILASSPIFVAIFGALFKIERLTALAWFGIVLSFVGIAIVIFGNAPTDQLTDQPGKN
                     ILLGDVLSLGAAIAWGAYTILVAPVVQHHSPIRVSAISIMFGTIPLVLLATPTFLTLD
                     WSKVGLNSWLGVLYSGGLAIAVGYVLWNRGVRHIGSARTAVYSNLNPVLAAIIAFFVR
                     GDALTIYHLIGAVVILAGIALTRLGRSQVMIEIEPEPIEG"
     gene            complement(1796..>2293)
                     /locus_tag="VFF70_02465"
     CDS             complement(1796..>2293)
                     /locus_tag="VFF70_02465"
                     /inference="COORDINATES: protein motif:HMM:NF028342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY43588.1"
                     /translation="GSDLLGLRTIMIIFGTRVRHTTLSSGQFYCPNCKTQRNYELRRA
                     RNYFALYFIPIIPMNTVGEYVACQTCGTNFKPDVLKLSPPAATAMDRITLETRADLDN
                     GTPIEFAKQKLINTGLKLELIDQTIAAAAGPDRSRCPKCSLTYRSTVKRCAQDGSDLL
                     PTVSA"
BASE COUNT          575 a          632 c          595 g          491 t
ORIGIN      
        1 agaggcacgg agaactaaat aaaactctgt gtctccatgc ctctgtggtg aatctattcc
       61 ggcaagtaga ctcgatcgcc gacggagaca accaacggtt ggtcactatt cgtcggcagg
      121 gtgtaataac cgcgcaggcc gccatcgagg aattgcagcg tttccttgat caccgccgtg
      181 tctctttcgg cttgcggcat ctggcttgaa tagcggctga attcgacgca cggtaacgca
      241 actttgatct ggcccagcca caaataatcg tcgcttgatc caagtcgaaa tgcaaggccg
      301 cgcgataaaa cgccggggcc gatcggtcga tcggtttcga tcaagatgtt ctcgcccgca
      361 cagccgatga acatatgatc gccgaatcga tcgcgcatct tcttatagtt cgccgcgaag
      421 ccgatcgaga tgctattatc acccgcattt ttcgaatcag gatgatcgac atgatggaca
      481 tcgatcgccg tgtcgtcatt tagcagtaag gcctgcgcgc ctttgggcgt cacgcgtaat
      541 cgatcgacac tgaggattgg cgacggatca tatatgcggt aaggcttttg tccaaccttc
      601 aagcctttgc gctgaatttg taaacggacg atggttccaa tttcaacaag attcatttag
      661 cccccgacaa taaaccactg attacacgga ttacacagat tttatttttc ccctcagtga
      721 catccgtgcc atccgtggtt gagtttttct gcgtggatca gatttaccct tcgattggct
      781 ctggctcaat ctcgatcata acttgactgc ggccaagcct tgtgagcgcg atgccggcca
      841 agatcaccac cgcgccgatc aagtgataga tcgtcagcgc atcaccgcgc acgaaaaagg
      901 caatgatcgc ggctaacacc ggattcaaat tcgaatagac cgccgtgcgc gcactgccga
      961 tgtgccgcac acctcgattc cacaacacat agcccaccgc gatcgccaat ccgcccgaat
     1021 agagcacacc caaccaacta ttcaagccga ccttcgacca atcgagtgtc aagaaagtcg
     1081 gggtggccaa caacactaat gggatcgtcc cgaacatgat cgatatggcc gacacgcgaa
     1141 tcggtgaatg atgctgcacc acgggcgcaa ctaagatcgt gtacgcaccc caggcaatgg
     1201 ccgcgccaag cgataggaca tcgcccagca aaatgttctt gcctggctga tcggtcaatt
     1261 gatcggtagg cgcattgccg aaaataacga tcgcaatgcc aacaaatgac agcacaatgc
     1321 cgaaccacgc caacgcagtc aaccgctcga tcttgaacag cgcgccaaag atggcgacga
     1381 agatcggcga actcgccaag atcaaggctg agtttgtcag cgatgtgtta tcgatgccaa
     1441 taatgaacat gacttgatag aggccattgc ccagcaagcc aagtccggcc atcttcaaca
     1501 catcgcgccg agtcagtggc tgctcgtcgc ggaacgaacg catcacgagc agcatgccaa
     1561 tcgtcgccag cgtaaatcga agcgcagtaa aggcgatcgg cggcaaatcg ccgctgatga
     1621 tagtcttgac cacggtaaaa ttgatcgccc acaaaccgat cgtcattaag agcagaagat
     1681 cggtcgggcc aaagctagac gatcgagaat tcatgtgtat gtcattctct ccaagaagat
     1741 agcagattgg gctcccatgc ccaatcgttc gacggcgtgg gagccgtcgc cgcaattaag
     1801 cggaaacagt tggcagcaaa tccgatccat cttgcgcgca gcgcttcacc gtcgatcgat
     1861 atgtcaacga gcatttcggg cagcgcgatc gatcggggcc agcagcggcc gcgattgttt
     1921 gatcgatcaa ttccaatttc aaaccggtgt tgatcaattt ctgtttggca aactcgatcg
     1981 gtgtaccgtt gtccagatcg gcccgcgtct ccagcgtgat gcgatccatc gccgtggcag
     2041 ccggcggaga cagcttcaac acatcgggct tgaaattcgt gccgcacgtt tggcaggcca
     2101 catattcgcc gacggtgttc atcggaatga tcggaatgaa gtacagcgca aaataatttc
     2161 gcgcgcggcg cagttcgtag ttgcgctgtg ttttgcaatt ggggcaatag aattggccgg
     2221 aagagagcgt ggtatgccgg acgcgtgtgc cgaagatgat catgattgtc cttaacccga
     2281 ggaggtcact gcc
//
LOCUS       DASRTJ010000119         6096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_44900,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000119 DASRTJ010000000
VERSION     DASRTJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44900"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFF70_02470"
     CDS             complement(<1..221)
                     /locus_tag="VFF70_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY43589.1"
                     /translation="MEKLSPHVYVESGYRGCTVGAIVTPEGVICIDTPLLPADARHWR
                     GQLAQLTDKPIRYVIFTDGHRDRVIGQQWL"
     gene            complement(299..1504)
                     /locus_tag="VFF70_02475"
     CDS             complement(299..1504)
                     /locus_tag="VFF70_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZY43590.1"
                     /translation="MTDRLYYSNSYLHDFEASIVDRLDHAGQPAVILDRSAFYPDSGG
                     QPSDRGTLNSARVIDVLEREADGEVLHILSEPIVGDRVSGSIDPVRRFDFMQQHTGQH
                     ILSQAFIQVAEAETVSFHLNSEAEGVVTIDLNTLDLPANQIDRVEDVANSIIADNRSI
                     IARFVTVAELESLPLRKPPKVTQSIRVVEVKDFDWSACGGTHVAHTGEVGLIKITKLD
                     RRGRETRVEFRCGRRALIDYRAKSRMINRVSADLTIGYWELDQALARLQADGKATRKQ
                     LVEAQARLQEYEAAEMLKAIETHGEYGLITRVWIDRDAADLKRMAQLLIARPKTVALL
                     GATGQALALTFARSKDLSIDLAVLLKAAALRVGGKGGGSPDFAQAGGPVVSEGQIKQT
                     LEWAVEQLK"
     gene            complement(1531..3441)
                     /locus_tag="VFF70_02480"
     CDS             complement(1531..3441)
                     /locus_tag="VFF70_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43591.1"
                     /translation="MTDKPILIVDSSIESRAWLIANVLDPAGYAHIEVPRLDEAHAKI
                     SRFQPAVIIFAALNDLVAATSFIAQYATSIPIIVIAATPSLEEVQAELRAGARDVLVR
                     PLEADRLAQAIERIRRQLHVSQERDTLLVEAKDRSEEFNALHAVGKKTAALLDLEEIL
                     TTVVGAAVTLTRAEQGSLMLLDPETGELYLRASQNLAEPGAQRLRIKVNDSLMGQVIQ
                     SGRPVMLSGNDLVKIRTSFLVKALLCVPLAVGGKTIGVLSVDNKISSRTFSDHEVNLL
                     STFADYAAIAIENSRLYPSAESERANLNTVLRDIQDAVIVVDHDMRILLVNQAAREAF
                     KLAGQVVGQPLITLIQHPRVIALFDQHKLRPKNWQAEIALEDGRILQGYLSVLNGIGF
                     GAIMHDITRLKELDRIKSEFVSIVSHDLRTPLTAIRGYVSLLSRAGPLNSQQQEFVTR
                     VENSMTNIVDLIADLLDIGRIEAGLDWEMESIQLQTVIHEAAQQQQPGAAAKHQTLTV
                     KVPDLSPILGNARRLRQVAANLIGNAVKYTPDNGRVEVLLREDSDFLVLQVRDTGIGI
                     SLEDQRRIFDKFYRVESELTDNISGSGLGLAIVKAIVEKHGGRVWVESEMGKGSTFTV
                     LLPKHSKITRTT"
     gene            complement(3498..4085)
                     /locus_tag="VFF70_02485"
     CDS             complement(3498..4085)
                     /locus_tag="VFF70_02485"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZY43592.1"
                     /translation="MDNTQHSISVIILAGGQSRRMGRDKALIDYQGKPIIAHVIDTLR
                     ELSDDVIVVSNQSEAYAQFGARIVPDYDPPSGPLGGIYTGLSAAKNDLAIVVACDMPF
                     LNRSLLRHLIDRAQQADAVVMLTDGEYEPLHAVYRRTCLPSIQDHLLKGDRRVISFFD
                     DVRLVTIPADQWRVIDPSGQSIANLNTPEDLQQLQ"
     gene            4284..>6096
                     /locus_tag="VFF70_02490"
     CDS             4284..>6096
                     /locus_tag="VFF70_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZY43593.1"
                     /translation="MALGGYADHVGHIDLSSGKVTYAAIPEEWKLKYIGGRGVGVKYV
                     FENGPKVDPLSPANILCFMNGPLTGSEANMSGRMAICTKSPLTGTITDSHHGGWSAAR
                     LRWAGFDGLIFKGKATKPVYVYVHDGAVEILDASEVWGKGVHDTVKHFKAKYGEKDLS
                     VIAIGQGGEHLVKFACWVNEDDRASGRGGTGCVGGSKNLKAVVIKAEKKMVKAADRDK
                     WKAAHQKALAEIMDERVVTAPRKGGLSVYGTNVLMNMTNVIGGLPAKNSQFTAFGDQA
                     ELISGEYVKQNILVDDPTCHACPVACKKEVEVKEGPWKGLHMESVEYEPAWSVGANCG
                     NSDINTVAKMIDLCNDYGIDAIETGHPLSIWMEATQRAYTNGDGGLKWGDIYGMTDTV
                     RKIAFREGIGNIMADGANVIAKHFGHPELAMTVKGQGIPAYDPRGIKGMGLGYATSNR
                     GACHLRAYTPAAEVIGNVLGPSTITDPLAWKGKGELTIIFQNVHTVTDCLDVCKFATF
                     AESLDSFAAQYEAITGVESNAGHLLKVGERVYNLERYYNNLNGFGEGSDYLPDRFTKE
                     ASTMPGSMGQVCELDQMLVEYYDKRGWKNGVVPMEKLK"
BASE COUNT         1385 a         1745 c         1709 g         1257 t
ORIGIN      
        1 agccactgct gaccgatcac ccgatcacgg tggccgtcgg tgaagatcac gtagcggatc
       61 ggcttgtcgg tcaactgcgc caattgcccg cgccagtgac gcgcatcggc cggcagcaaa
      121 ggcgtatcga tgcagatcac tccctcgggc gtgacgatcg cgccgaccgt gcagccgcga
      181 taaccggatt caacgtaaac gtgcggggaa agtttttcca tataggttct cgatgatcat
      241 cagaattcaa tgaacggtgc gtgttgcgat gttaggcagt ctacgacacg ctccccattc
      301 atttcaactg ctctaccgcc cactcaagtg tttgcttgat ctgaccttcg gagaccaccg
      361 ggccgccggc ctgggcaaag tccggtgagc cgccaccttt gcctccaact cgaagagcgg
      421 cagcttttaa caataccgct agatcgatgg agagatcttt cgatcgagcg aatgtgagcg
      481 cgagcgcttg gccggtagcg cccaacagcg ccacagtttt cggtcgagcg atgagcagct
      541 gcgccatacg ctttaggtca gcggcatccc gatcgatcca cacgcgagtg atcaagccgt
      601 actcgccatg cgtttcgatc gctttcaaca tttcggcggc ctcatactct tgcagacgcg
      661 cctgcgcttc gaccaactgc ttgcgggtcg ctttgccgtc cgcttgcaat cgcgccagcg
      721 cttgatcgag ttcccaataa ccgatcgtga gatcggcgga tacccgattg atcatccggc
      781 tcttggcacg ataatcgatc agcgcgcgcc ggccgcagcg aaactccacg cgcgtttcgc
      841 ggccgcgccg atcgagtttg gtgatcttga tgagcccgac ttcgcccgta tgtgccacat
      901 gggtgccgcc gcaagccgac cagtcaaagt ctttgacttc gacaacgcga atggactgag
      961 tgactttcgg cggcttgcgg agaggaagag actccaactc tgccacggtc acgaagcggg
     1021 cgatgatcga tcggttgtcg gcgatgatcg aattggcgac atcttcaacc cgatcgattt
     1081 gattcgccgg aagatcgagc gtgttgagat cgatcgtgac cacaccctcc gcctcagaat
     1141 tcaagtgaaa tgaaacggtt tcagcttcgg ccacttgaat gaaggcctgt gacaaaatat
     1201 gctggccggt gtgttgctgc ataaaatcga aacggcgcac cggatcgatc gagccggaga
     1261 cgcgatcccc gacgatcggt tcggacaaaa tgtgcagcac ttcgccatca gcttcacgct
     1321 cgagtacgtc gatcacgcgc gcgctattca gcgtgcctcg atcggacggc tggccgccgc
     1381 tgtcgggata gaaggctgat cgatctaaga tcaccgcggg ctgtccggcg tgatcgagcc
     1441 gatccacgat gctagcctcg aagtcgtgca gatatgaatt ggaatagtag aggcgatcag
     1501 tcatctgcaa gtttaaccaa taacgagtaa ttacgtcgtc ctcgttattt ttgagtgttt
     1561 cggcagcaac accgtgaacg tgctgccttt gcccatctca ctttcaaccc aaacgcgacc
     1621 accgtgcttt tcgacgatcg cttttacaat cgccagaccc aggccactgc cgctgatgtt
     1681 atcggtgagt tccgattcca cccgatagaa tttgtcgaag atgcggcgct gatcttcaag
     1741 cgaaatgccg atgccggtat cgcgcacttg cagcaccagg aagtcactat cctcgcgcag
     1801 taggacttca acgcggccat tatccggcgt gtacttgacg gcgttaccga tcaagttagc
     1861 agccacctgc cgcagacgcc gcgcattacc caggatgggc gacagatccg gaactttgac
     1921 cgttaaggtt tggtgtttgg ccgcggcgcc gggttgctgc tgctgcgccg cctcatgaat
     1981 gacggtttgc agctggatgg attccatctc ccaatcgagg ccggcttcga tgcggccgat
     2041 gtccagcaaa tcggcgatca agtccacgat attggtcatg ctgttttcaa cgcgcgtcac
     2101 aaattcctgc tgctgcgaat tcaacggacc ggcgcgcgat aacaaactca cgtaaccgcg
     2161 aatggccgtc agcggcgtgc gcagatcgtg cgagacgatc gacacaaact cgctcttgat
     2221 gcgatccagt tctttcaggc gtgtgatgtc gtgcatgatc gcgccgaagc caatgccgtt
     2281 caacaccgaa agatagcctt gcaagatgcg gccatcctcc aacgcgatct cggcttgcca
     2341 attcttcgga cgcaatttgt gctgatcgaa cagagcgatc acgcgcggat gctgaatcag
     2401 tgtgatcagc ggctggccaa caacctgccc ggctaacttg aacgcttcgc gggcggcctg
     2461 gttgaccaat aagatacgca tatcatgatc gacgacgatg actgcatcct gaatatcgcg
     2521 cagcacggtg ttgagattgg cgcgctcgct ctcagccgaa gggtacaagc gcgaattctc
     2581 gatcgcgatg gcggcatagt cggcaaaggt ggataacaag ttgacttcat gatcgctgaa
     2641 ggtgcgactg gaaattttgt tgtccacgct taacacgcca atggttttgc cacccaccgc
     2701 caacggaacg cacagcaggg ctttcaccag aaaggaagtc cgtatcttta ccaagtcatt
     2761 gccgctgagc atgaccggcc gaccgctctg aataacttgg cccatcaaac tatcgttgac
     2821 tttgatgcgc agccgctgcg cgcccggctc ggccagattt tggctggcac gcagatacag
     2881 ttcgcccgtt tccggatcga gcagcatcaa cgatccctgc tcagcgcgcg tcagtgtgac
     2941 cgccgcgcca accaccgtcg tcaagatttc ttcgaggtcc agcagcgcgg ctgtcttttt
     3001 gccgacggca tgtagggcgt taaattcttc agatcgatct ttggcctcaa cgagcagcgt
     3061 atcacgctcc tgcgacacgt gaagttgtcg gcggatacgc tcaatcgctt gcgctaaccg
     3121 atcggcttcc agcggcctaa ccaaaacatc gcgggcgccc gccctcaatt cagcctgcac
     3181 ttcctccaac gaaggcgtcg ccgcgatcac aatgatcgga atcgaggtcg cgtattgggc
     3241 aatgaaagag gtcgcggcga cgagatcatt caaggcggcg aaaatgatca ccgcaggctg
     3301 aaaacggctg atcttcgcat gagcttcatc cagtcggggg acttcaatat gcgcatagcc
     3361 ggccggatcg agcacgttgg caataagcca ggcacgcgat tcgatgctgc tatctacaat
     3421 aagaattggt ttgtcagtca agaaaccctc gcaattaaat taacatcttg aatgatagca
     3481 caaaaagagg cgcgagacta ttgcagctgc tgtaaatctt cgggggtgtt caaattcgca
     3541 atcgactgcc cggacggatc gatcacacgc cattggtctg cggggatcgt taccaatcgc
     3601 acatcatcaa agaaactgat cacacgtcga tcgcccttga gcaaatgatc ttgaatcgag
     3661 ggcaagcagg tgcgtcgata aacggcatgc agtggttcat attcaccgtc ggtcagcatc
     3721 accactgcat ctgcttgctg agcacgatcg atcaagtggc gcagcagcga tcgattcaag
     3781 aaaggcatgt cacacgccac gacgatggcg agatcgtttt tcgctgccga tagtccagtg
     3841 taaatcccac cgagcggccc gctgggcgga tcgtagtccg gcacaatacg cgcgccgaac
     3901 tgcgcatacg cttccgattg attcgacacg acgatcacat cgtccgataa ttcgcgcagc
     3961 gtatcgatca cgtgcgcgat gatcggcttg ccctgataat cgatcagcgc tttgtcgcgg
     4021 cccatgcgcc ggctttgtcc gcccgcaaga ataattacgc tgatgctgtg ctgtgtatta
     4081 tccacaattc gatcatactc tgcccgtggg cgtacgtcaa acgtactcgg acaggcccat
     4141 tattggcgtt atcctaatcg tgcataacgg gggtgacata gatcacttga aaaacgaacc
     4201 aaacggggct gttgggagga atcacttgat cttaaactaa gctgcaagtc tcacaacata
     4261 aacacttcaa tctggaggtt accatggcac ttggcggtta tgctgatcac gtcggtcaca
     4321 tcgatctcag ttcgggcaaa gttacttacg ccgcaattcc cgaggagtgg aaactgaagt
     4381 acattggggg acggggcgtc ggcgtgaagt atgtgtttga gaatggcccg aaggtggatc
     4441 ccctttcacc tgcaaacatc ttgtgcttca tgaatggtcc gctcacaggc agcgaagcta
     4501 atatgagcgg acgcatggcg atttgcacca agtcgccact caccggcacg atcaccgatt
     4561 cgcaccacgg cggctggtca gcggcccgct tgcgctgggc cggctttgac ggcctgatct
     4621 tcaaaggcaa agccaccaag ccagtatatg tctacgtgca cgatggtgcc gtcgaaatcc
     4681 tcgacgcttc ggaagtgtgg ggcaaaggcg tgcacgacac ggtcaagcac ttcaaggcca
     4741 agtacggcga gaaggatctc agtgtgatcg ccatcggcca gggcggtgag catctggtca
     4801 agtttgcctg ctgggtcaac gaggacgatc gcgccagcgg ccgcggcggc acgggctgcg
     4861 taggcggcag caagaatctg aaagccgtcg tcatcaaagc cgagaagaaa atggtcaagg
     4921 ccgctgatcg cgataagtgg aaggcggctc accaaaaagc gctggccgaa attatggatg
     4981 agcgtgtggt cactgcaccg cgcaagggcg gcttgtcggt atatggcaca aacgtgctga
     5041 tgaacatgac caacgtcatc ggcggtctgc ccgctaagaa cagccagttc acggccttcg
     5101 gcgatcaagc cgaattgatc agcggcgagt acgtcaagca gaacatcctc gtcgatgatc
     5161 caacctgcca tgcttgcccg gtcgcctgca agaaggaagt cgaagtcaag gaaggccctt
     5221 ggaagggctt gcatatggaa agcgtcgagt acgagccggc ctggtcggtg ggcgccaact
     5281 gcggcaacag cgacatcaac accgtcgcca agatgatcga cctgtgcaac gattacggca
     5341 tagacgccat cgagactggc cacccactgt ccatctggat ggaagcgact cagagggcct
     5401 ataccaacgg cgacggcggc ctgaaatggg gcgacatcta cggcatgacg gacaccgtcc
     5461 gcaagatcgc cttccgcgag ggcatcggga atatcatggc cgacggcgcc aatgtcatcg
     5521 ccaagcactt tggtcatccc gaactcgcca tgacggttaa gggccaaggc atcccggcct
     5581 acgatccgcg cggcatcaag ggcatgggtc tgggctatgc caccagcaat cgcggcgcat
     5641 gccacctgcg cgcttacacg cccgctgctg aggtgatcgg caacgtgctc ggcccgtcga
     5701 cgatcaccga tccgttggcg tggaaaggca aaggcgaact gacgatcatc ttccagaacg
     5761 tgcacaccgt caccgactgc ctcgatgtgt gcaagttcgc cacgttcgcc gaatcgctcg
     5821 attcgttcgc ggcgcagtat gaggccatca ccggcgtcga gtccaatgcc ggtcacctgc
     5881 tcaaagtggg cgagcgcgtc tacaatctgg agcgctacta taacaacctc aacggcttcg
     5941 gcgagggcag tgactacctg cccgatcgct tcaccaagga agcgtccacc atgcccggtt
     6001 cgatggggca ggtgtgtgaa ttggaccaaa tgttagttga gtattacgat aagcgcggct
     6061 ggaagaacgg cgtcgtgccc atggagaagt tgaaag
//
LOCUS       DASRTJ010000120         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_309768,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000120 DASRTJ010000000
VERSION     DASRTJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309768"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFF70_02495"
     CDS             complement(<1..1220)
                     /locus_tag="VFF70_02495"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZY43594.1"
                     /translation="MNEAKKFSTTLGRDTITLETGKLANQAGGAVTVRLGDTLVLVTA
                     TMAKSPREGIDFFPLSVEYEERQYAAGRIPGSFFRREGRPSESAILVCRLTDRPLRPL
                     FPEGFRNDVQVVITSLSSDSEHQVDILAINGASAALTISDIPFQGPVGAVRVGYIDGQ
                     FVVNPTSSELDTSQLDLRIAGTADAILMVECGANELPEALMVEALEFGHKALQPMIEL
                     QNKMRAVLGKPKNESYPMFGVPTDVTATVRAKIGKDIEAIFDKGGNKSGINDALEELQ
                     TSIVEAFEDKIDSALVKNVFHDVEKDVVRSRILDRGLRPDGRGLTDIRPLAAEIDISP
                     RAHGSGLFTRGETQVLSMATLGTLKDAQMLDGLDVDTTKRYMHHYNFPPYSTGEARPM
                     RGSSRREIGHGALA"
     gene            complement(1516..1782)
                     /gene="rpsO"
                     /locus_tag="VFF70_02500"
     CDS             complement(1516..1782)
                     /gene="rpsO"
                     /locus_tag="VFF70_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZY43595.1"
                     /translation="MLAKDEKTKIITDFHQHETDTGSAEVQIAILTARITQLTGHLNI
                     HKHDQTSRHGLLRMVGERRRHLAYLSRTNPTRYKDILQRLGLRK"
     gene            complement(1959..2786)
                     /locus_tag="VFF70_02505"
     CDS             complement(1959..2786)
                     /locus_tag="VFF70_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZY43596.1"
                     /translation="MPELPELEVVKEVLQRRAVGQSIEAVRIDPKGGPIVIRDLTHRG
                     FEQTLIGSSINGVTRRGKFMLFSIDPPVHLVLNPKLTGRLQLTTPNQKRMPKTHIVFS
                     LSGGDELRYVDQKTMGQVYLTDDLNQITDYPGLGPEPFDMTLDQFRERLKPYRGEIKG
                     VLVRGEFIAGIGNAYADEILWTAKIHPYRKRTQLSGEEIDRLYQAIQSTLREAIDKVR
                     IEMADNIHLKPREFMAVHMKTGEPCPRCGTSISLVGANQRITNFCRTCQPGGLIKGM"
BASE COUNT          615 a          763 c          814 g          701 t
ORIGIN      
        1 tcagccagcg cgccgtggcc gatctcgcgt cgagacgagc cgcgcatggg cctggcttca
       61 ccggtggaat acggcgggaa gttgtagtga tgcatgtagc gcttggtcgt atccacgtcg
      121 aggccgtcca gcatttgggc atccttcaag gtgcccagcg tcgccattga tagcacttgc
      181 gtttcgccgc gcgtaaacag gccagagccg tgcgcgcgcg ggctgatatc aatttcagcg
      241 gctagcggtc gaatgtcggt caggccgcgt ccgtcgggcc gcagaccccg atcgagaata
      301 cgggaacgca cgacatcttt ttcgacatcg tgaaaaacat ttttgactaa agccgaatcg
      361 atcttatctt cgaacgcttc cacgatcgat gtctgcaatt cctcgagcgc gtcgttgatg
      421 cccgatttat tgccgccctt atcaaagatc gcttcgatgt ctttgccaat cttcgctcga
      481 actgtggcgg tgacgtcagt cggtacgcca aacataggat acgattcatt cttcggcttg
      541 cccaacaccg cgcgcatttt attttgcagt tcgatcatcg gctgcaaagc tttgtggccg
      601 aactccagcg cttcaaccat caaggcttcg ggcagttcgt tcgcaccgca ttcgaccatg
      661 aggatcgcat cggcggtgcc ggcgatccgc agatcgagtt ggctggtgtc caattccgat
      721 gacgtgggat taaccacgaa ctgcccatcg atgtagccga cgcgcaccgc gccgaccgga
      781 ccttgaaacg gaatgtcgct gatcgtcagt gcggccgacg cgccgttgat ggcgagaata
      841 tcgacttgat gttccgagtc ggatgacagt gaagtgatga cgacttgcac gtcgttgcga
      901 aagccctccg ggaacagcgg gcgcagcggc cgatcggtta agcggcatac caaaatcgcg
      961 gactcagagg gacggccttc gcgccgaaag aatgatccgg ggatgcggcc ggcggcatat
     1021 tgtcgctctt catattccac gctgagtgga aagaagtcga tgccttcgcg cggcgatttc
     1081 gccatggtcg ccgtgacaag gacgagtgta tcgcccaggc gcaccgtgac cgcgccaccg
     1141 gcttgatttg ccagcttgcc ggtttcgagc gtgatggtgt cgcgcccaag ggtagtgcta
     1201 aatttttttg cttcgttcat gttttgacct cctgaggtcg atatcaagat gggcagtgga
     1261 ggtcagggac tggagagaag aatgaacaat gtacaatttt caatgtgcaa tgaacaattc
     1321 gcgtttgctc attgtcaatt gttaattgct aaatgttaat tgctcgctcc aattcctgct
     1381 cactccattg cgatagatgg ttgatgatga tttaagctgt gactgagtct cctcatcacc
     1441 gaatggaaac gcaaagagag tagatggcaa tgtcacagcc acaactcact cgcgatgttc
     1501 gattagccga ttgaattact tgcgcaggcc tagacgttgg aggatgtcct tgtaacgcgt
     1561 cggattggtg cggctcaagt acgccaagtg gcggcgtcgc tcgccgacca tccgcagcag
     1621 gccgtgccgc gaggtctgat cgtgcttgtg gatattcaga tgtcccgtca actgagtgat
     1681 gcgcgcggtg agtattgcaa tctggacttc ggccgagccg gtgtccgtct catgttgatg
     1741 aaaatcggtg atgatcttcg tcttttcgtc tttcgccagc atgttccata ctccttagtg
     1801 atatcacgat cgcagcggct gtgacacttc gtcgtgtgat tcggttgatg ggcaggattc
     1861 taccacgaat aagcggttgg tacaaacggg catactgaag aagcatggcg cgtgatgcgt
     1921 gacgagtgat atgtgaaaaa caaatcacgc atcacgtttc acatcccctt aatcaatccg
     1981 cccggttgac aggtgcggca gaaattggtg atgcgctgat tcgcgccgac taatgagatc
     2041 gatgtgccac agcgcgggca tggctcgcca gtcttcatat gcaccgccat aaattcgcgc
     2101 ggcttgagat ggatgttatc cgccatctcg atccggactt tgtcgatcgc ttcacgcaag
     2161 gtcgattgga tcgcctgata caaccgatcg atctcctcgc ccgacagttg ggtgcgcttg
     2221 cgatacggat gaatcttcgc tgtccacaga atctcatcgg cataggcgtt gccgatgccc
     2281 gcgataaatt cgccgcgcac caagacgcct ttgatctccc ctcgataggg cttcaatcgc
     2341 tcgcggaatt gatcgagcgt catatcgaac ggctcggggc cgagtccggg ataatcggtg
     2401 atttgattga gatcatcggt caaatacact tggcccatcg tcttttgatc gacgtagcgt
     2461 agttcatcgc cacccgataa tgaaaagacg atgtgagttt ttggcatccg tttttgattt
     2521 ggcgtagtca attgtaaacg cccggtgagt ttgggattga gcaccagatg gacgggagga
     2581 tcgatcgaga atagcataaa cttcccgcgc cgcgtaacac cattgatcga tgatccgatc
     2641 aaagtttgct cgaagccgcg atgggttaga tcgcgaatga cgatcggccc tcccttggga
     2701 tcgatccgca ccgcctcgat cgattgtccg acggcgcggc gctgcaagac ttctttgacg
     2761 acttcaagtt cggggagttc gggcataccg ctcgcttgtt ggctcgtgcg aatctatgct
     2821 atcatcgact tcgatgtgga ttatacgcta atgcggccag agtcatgccc gaatgcttct
     2881 atcgggcatc cag
//
LOCUS       DASRTJ010000121         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82457,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000121 DASRTJ010000000
VERSION     DASRTJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82457"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VFF70_02510"
     CDS             complement(<1..2360)
                     /locus_tag="VFF70_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZY43597.1"
                     /translation="MQLNAIIVALQDWRGKSAMHIDLIGSLSWYIQIPVRPEGWVLLG
                     LYLLITLACLFYWRRTFTRLPSRRWLGFIAFAFAAIVLCVILVWSFPLNTSLPLPDSP
                     QRADIPATPLLSSVVILLAIIWLDIGPALVLSLISGVLLSGFYSGQFTLRFEVVASAI
                     IAGYWLRQNYHGRVNYFLRQPIISGPLAALITWPLLLFAFYSFMPGDTLPALNTAWLL
                     FLSSFWPTMAVGLIAGGVAQLSLSVFPKWRLPMPEPLPPPWARSLNRRMLFTFIPFGA
                     LLVIVLVYAVSATALSGARQQAIDQMSHDAQSAAREVPNFLTMGQGLIHEFGTDDRLS
                     SPDRTVRETRLASVVRIGPFFDELLEFDAAHQLIAAYNQGPDLTPEEQTLISRTLLTR
                     APQTSEVFALGSDSAISFVDAQLDPNTGQPQGAVLGRTRLQRSVLMTLLQKNLQNTLG
                     AGVGYLIDRENRIIVHPNPALALTPAPFASDLTPTAVTTQSGRAYDDHAPNGAPRLLF
                     VQPIEGTDNWSVVIELPFDKVLQIAAEIFKPLLVFLLLLGFVAAVVLSFFTRAITRPV
                     QQLSDAAGNIARGQLDRPIVPSGQDEVGQLGSAFEQMRQSLKGRLDDLSLLLHVSQTV
                     SSSLDLERGVPPLLSGALQATPARVARLVLLSDNGSPEAVLTSTESPALPTPLDTALV
                     ALTAQNEAPLLIDNVTRARSRAALEPNLIGPGIKAIAALPVRRQTKPIGLMWLGYAEA
                     HDFTESEVNVLGTLAGQAAVLIENAKLFLAAEGGRRRLQAVLTSTS"
BASE COUNT          632 a          707 c          663 g          495 t
ORIGIN      
        1 tcgctggtgc tggtcaacac cgcttgcaag cggcggcgtc caccttcggc cgccaggaac
       61 aacttcgcat tttcgatcaa cacagcggct tgaccggcga gtgtgcccaa cacattgact
      121 tccgattcag tgaagtcgtg cgcttccgcg tagccgagcc acattaatcc gatcggtttg
      181 gtctgccgcc gaacaggcag tgcggcgatc gctttgatgc ccggcccgat cagatttggt
      241 tcaagcgccg cgcgcgatcg cgctcgcgtc acattatcga tcagcaatgg tgcttcgttc
      301 tgggcagtta gcgccaccaa tgctgtatct aacggtgtcg gcagtgccgg actttcagtc
      361 gaggtcaaca ctgcttcagg cgatccgttg tccgataaca gcaccaatcg agccacacgt
      421 gctggcgtgg cttgcaacgc accgcttaac agcggcggca ctccacgttc cagatcgagc
      481 gatgacgaaa cagtttgact gacatgcagc aacaaagaga gatcatccaa gcggcctttc
      541 aggctctgcc gcatctgctc gaacgcgctg cctagttgtc cgacttcgtc ttgaccgctg
      601 ggtacaattg gccgatcgag ttggccgcgg gcgatgtttc cggccgcatc agacaactgc
      661 tgaaccggcc gcgttatcgc tcgcgtgaaa aacgagagca caaccgccgc gacaaatccc
      721 aatagcagca agaagaccag cagcggctta aaaatttctg cggcgatctg gagaacttta
      781 tcaaatggca gttcaatgac caccgaccaa ttgtccgtgc cctcgatcgg ttgaacgaac
      841 aacaaacgtg gcgcaccgtt cggcgcatga tcatcatacg ccctgccgct ctgcgtggta
      901 actgccgtcg gtgtgagatc agaagcaaac ggagcgggcg tcagcgccaa ggctggatta
      961 gggtggacga taatccggtt ttcgcggtcg atcaaataac cgacgccagc gcccaatgtg
     1021 ttctgtaaat ttttttgtaa cagcgtcatg agcacactgc gttgaaggcg ggtgcgacct
     1081 aacactgctc cctgcggctg gccggtgttc ggatcgagct gcgcatctac gaacgatatg
     1141 gcgctgtcgc tgccaagcgc aaagacctcg ctcgtttgcg gtgcacgggt cagcaaggtg
     1201 cgtgaaatta gcgtctgttc ttcgggtgtc aagtctggtc cctgattata ggcggcgatc
     1261 agctgatgtg ccgcatcgaa ttccagcaac tcatcaaaga acggaccgat gcggacaaca
     1321 ctggccaatc gggtttcacg cacagtccga tcgggactgc tcaatcgatc gtccgtgcca
     1381 aattcgtgaa tcaagccttg acccatcgtg agaaaatttg gcacttcccg cgcggccgat
     1441 tgtgcatcgt gcgacatttg atcgatcgct tgttgccgcg ccccactcaa cgccgtggcg
     1501 ctgacggcat acaccagaac gattaccagc agcgcgccaa atggaatgaa agtaaataac
     1561 atgcgccgat tcaagctgcg cgcccacggc ggcggtaacg gttccggcat tggcaaacgc
     1621 cacttcggga aaacagagag tgacaactgg gccacaccgc cagcgatcaa acctacggcc
     1681 atcgtcggcc aaaagctgct cagaaacaat aaccacgccg tattgagtgc gggcagtgta
     1741 tcacccggca taaagctata gaacgcaaac agcaacaatg gccatgtaat caacgcggcg
     1801 agcggtccgc tgataatcgg ttggcgtaaa aaataattca cgcgcccatg ataattttgg
     1861 cgcagccaat aaccggctat gatggccgag gccacaactt caaagcggag tgtgaactgg
     1921 ccactgtaaa atcctgacag taggacaccg ctgattaagc ttaacaccag cgcaggacca
     1981 atatccaacc aaataatggc taataaaatg acgacgctgg ataacaacgg cgtcgccggg
     2041 atgtctgcac gctggggtga atccggcagc ggtagtgaag tattaagggg gaaagaccag
     2101 actagaatga cacacagaac aatcgcggcg aaggcaaacg caataaaccc caaccagcgg
     2161 cgcgagggca atcgagtgaa agtgcgccgc caataaaaca ggcaggccag ggtgatcagc
     2221 aaatataatc caaggagcac ccaaccttca ggccgcaccg gtatttgaat ataccaactc
     2281 agactgccaa tcaaatcaat atgcatggcg ctcttgcccc gccaatcttg cagcgcgaca
     2341 attatagcat taagttgcaa gataccgcga atcgatcata tgcactttcc tgaaatttta
     2401 tgccgcacat gagtcaggtt cttgcatgaa tcgaccgagc gggttacaat atcgccgctc
     2461 cccattcgaa cggattaccc gaactttctc cgaatgg
//
LOCUS       DASRTJ010000122         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82461,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000122 DASRTJ010000000
VERSION     DASRTJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82461"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1562
                     /locus_tag="VFF70_02515"
     CDS             102..1562
                     /locus_tag="VFF70_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY43598.1"
                     /translation="MPHQNTDNNNQKLNKVIVIGGGPAGLTAAYELSKVGVKSIVLEM
                     GSRVGGIARTEVYKGYRMDIGGHRFFTKVGEVEALWQEILGGDFLVRPRLSRIYYDRK
                     YIMYPLRFFDALSKLGVWQSILILISYLRSKIFPYRAENNFEEWVSNRFGRRLYRIFF
                     KTYTEKVWGIPCSKLSAQWAAQRIQNLSLPRAVWSAVFGARGAKNVKSLIEEFNYPRL
                     GPGMLWERTQQLLGASGNEVRVNAEVVKVLHRSGRAIGVVVRSGGRAETIEADAVIST
                     MPISELIAQLDPPAPEDIQSGAGQLTYRDFITVGLIINQPDLFPDNWIYIHSPEVQVG
                     RIQNFKNWSPAMVPDPSKTFLGLEYFCLESDALWTMADADLIELGKQELDRMGLARAS
                     DVEDGTVIRQKKAYPVYTGEYKEYLERIRGFLDTIPNLQTVGRNGLHMYNNQDHSMLT
                     AMLAVKNLLGEQHDVWSVNVERSYHEEVQIPLTAEG"
     gene            1565..3850
                     /locus_tag="VFF70_02520"
     CDS             1565..3850
                     /locus_tag="VFF70_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43599.1"
                     /translation="MLARIKLSASLWFNLKTILAAYVLLGLLYAFTPPVFEKPDELWH
                     FAYIKLLADGRGFPTPPVVISDDTPAQEITQPPLYYLMAAPIVQVFVPDTTDFFGLLH
                     RNPSVGSSLDNNDNKNMFIHTDREAFPFHGTVLAIYILRVFSLLFGGLTIWFTYQLCL
                     EVWPQRSSTALLAAAIVAFTPEFVFVTSAVSNDGLSAAMGALVLWLTARLLRRGLTRR
                     RVLILGSALGLAALSKASALGLIPIVVFVVLMAAPAPDGQSLRWRERISGSLWMVLPL
                     LLIAGAWYVRAYVLYGDLLGTAIHELLPWARPQSISFGEALLQMPTIIRSYWLAYGWG
                     TIGPPQVFYSALNLFAAIGLIGALYTWYTVIVNRDRIVMWCLLALGGWLGVIVIALIR
                     WNQILLSPLGRLALPSIAAIGVLIAIGWTSIGSRVLIDRRWSFLQRIPWASAAPIGLF
                     SLVLIAIPIVLLPAYAPPQFLSVDQIAQQPGQATDVRFGNLTRLIRLEVPRDPAPQPG
                     ESVPLRLCWETLSSDARDLLVLIQFIGIENRVAATRRTVPGLGGYATVNWQAGRYFCD
                     DVDVLISSTASAPAVYKVEVSLIDFATQERMIAYAPNGSELSTDFVGAIKIAPQVYAS
                     PTIDHPLNIRLGDQVDLIGYAIEPITLDRGGVVHLRLYWRAVRPPDADYTVFVHLNDA
                     SGQTPAQADAPPQSGAYPTSFWAAGEVVIDDRDIVLPQTVSAGRYVLGVGLYNPIDGS
                     RLAVAGNASNQIDLPDQLEVK"
     gene            3847..>4034
                     /locus_tag="VFF70_02525"
     CDS             3847..>4034
                     /locus_tag="VFF70_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43600.1"
                     /translation="MTKSRWLFLALILLAFALRVYRLEAQSLWSDEGLSVYRARLILP
                     ENLTNVIVVPPNVPTQDT"
BASE COUNT          818 a         1046 c         1159 g         1011 t
ORIGIN      
        1 gttatcaagc gagtttcagt cggcctttat cgcctcgata ccggcgaacg cttaaccatc
       61 gacggcacaa acgaaaacga atttgtttta acgactccat aatgccacat caaaacacag
      121 acaacaacaa tcagaaattg aacaaagtca tcgtgatcgg cggcgggcca gcaggcttga
      181 ccgccgctta cgagttgagc aaggttggtg tcaaatcgat cgtactggaa atgggctcgc
      241 gcgtcggcgg catcgcgcgc accgaagttt acaaaggcta tcgcatggat attggcggtc
      301 atcgcttctt cacgaaagtc ggcgaggtcg aagcgttgtg gcaggaaatt ttgggcggcg
      361 actttttagt aaggcccaga ctcagccgca tctattacga tcgcaaatat atcatgtacc
      421 cgctgcgctt tttcgatgcg ctgtcgaagt tgggtgtgtg gcaaagcatt ttgatcttga
      481 tcagttatct ccgatcgaaa atctttccgt accgcgctga aaacaatttt gaagaatggg
      541 tctcgaatcg atttgggcgg cggctgtatc gaatcttttt caagacctac accgagaaag
      601 tctggggcat tccctgctcg aaactcagcg cgcagtgggc tgctcagcgc attcagaatt
      661 tgtcgctgcc gcgcgcggtg tggagtgccg tgttcggtgc gcgcggcgcg aagaatgtga
      721 agtcattgat cgaagagttt aactatccgc gtttaggccc cggcatgttg tgggagcgca
      781 cgcaacagct acttggcgcg agcggcaatg aagtgcgcgt aaatgccgag gtcgtcaaag
      841 tcctgcatcg atccggacgc gcgatcggcg tggtggttcg atcgggcggg cgcgcagaaa
      901 cgatcgaggc agacgccgtg atctcgacca tgccgattag cgaactcatc gcccagcttg
      961 atccgcctgc accggaggat attcaatcgg gcgccggtca attgacttat cgggacttca
     1021 tcaccgtcgg cttgatcatc aatcagcctg atttattccc ggacaattgg atctacatcc
     1081 attcacctga agtgcaagtg ggccgcattc agaacttcaa gaactggagc ccggcgatgg
     1141 tgcccgatcc gagcaagacc ttcctgggct tggaatattt ctgtctggaa agtgatgcgc
     1201 tgtggacaat ggcggatgcc gatctgattg aactgggcaa acaagaactc gatcgcatgg
     1261 gtctggctcg cgccagcgat gtggaagatg gcactgtcat tcgccagaag aaagcctatc
     1321 cggtttatac cggtgagtac aaagaatatc tggagcgtat tcgcggcttt ctggatacga
     1381 tcccgaattt acaaacagtc ggacggaacg gtctgcacat gtataacaac caagatcact
     1441 cgatgttgac ggcgatgtta gccgtgaaga atttgttagg cgaacagcat gacgtgtggt
     1501 cggtcaatgt cgaacgatcg tatcatgaag aagtgcaaat tccgttgact gccgaaggat
     1561 aggcatgcta gcccgcatta aattgtctgc gtccctctgg ttcaatctca aaacaatcct
     1621 cgcggcgtat gtgctgctgg gtttgttata tgcatttact ccgccggttt ttgaaaaacc
     1681 ggatgagctg tggcactttg cctatatcaa attgctcgcg gatggacgcg gctttcccac
     1741 gccgcccgtt gttatttcgg acgacacacc tgctcaagag atcactcaac ctccgctgta
     1801 ttatttgatg gccgcgccga tcgtccaagt gttcgtgccc gacacgactg atttctttgg
     1861 gctcctgcat cgcaatccct cagtcggatc ttcgctggat aacaatgaca acaagaatat
     1921 gttcattcac accgatcgcg aagccttccc ttttcatggg acggtgctgg cgatctacat
     1981 cctgcgcgtt ttctcgctat tatttggcgg gttgaccatt tggttcacgt atcaattgtg
     2041 cctggaagtt tggccgcagc gatcttcgac ggcattgttg gctgcggcga ttgtggcgtt
     2101 cactcctgaa tttgtgttcg tcacatcggc cgtgagcaat gatggtctct cagcggcgat
     2161 gggcgcactg gtgttgtggt tgaccgcccg cctgttgcgg cgtggcttga cgcgccgccg
     2221 tgtgctcatc ctaggcagcg ccttaggtct ggccgctttg agcaaagcca gcgcgttggg
     2281 cttgataccg atcgtggtgt tcgttgtgtt aatggccgcg cctgcaccgg acggtcaatc
     2341 gctgcgttgg cgcgagcgga tcagcggcag cctgtggatg gtgcttccgc tgctgttaat
     2401 tgccggcgca tggtatgtgc gcgcctatgt gctctacggc gatctattgg gcacggccat
     2461 ccatgaatta ttaccctggg cgcgtccgca atcgattagc ttcggtgagg cgctgctgca
     2521 aatgccaacc atcatccgat catactggct ggcctatggc tggggcacga tcggcccgcc
     2581 gcaagttttt tattctgcgc taaatttgtt tgcggccatt ggtttgatcg gcgcactgta
     2641 tacgtggtat accgtgatcg tcaatcgcga tcggatcgtg atgtggtgtc tgctggcatt
     2701 aggcggctgg ctgggtgtca ttgtgattgc gctgattcgc tggaatcaaa tattgctttc
     2761 acccctaggt cgtttggcgc tgccgtcgat cgccgcgatc ggtgtgctga tcgcgatcgg
     2821 ttggacatcg atcggatcgc gcgtgttgat cgatcgacgt tggagttttc tgcaaagaat
     2881 cccctgggca tcggcggcac cgatcggctt gtttagctta gtgctgattg cgatcccgat
     2941 tgtcttgctg ccagcttatg cgccgccgca atttttgagc gtcgatcaga tcgcccagca
     3001 gcccggccag gctactgacg tgcgctttgg taatctgacg cgcttgatcc ggcttgaagt
     3061 accgcgcgat cccgcgcccc agccgggcga gagtgtgccg ctgcgcctat gttgggaaac
     3121 gttatcgagc gatgcgcgcg atcttttggt gttgatccaa tttattggca tagagaatcg
     3181 cgtggccgcg acacgccgaa cggtgcccgg cctcggcggt tacgccacgg tcaattggca
     3241 agcgggccgg tatttttgtg atgacgtgga tgtgttgatc agttccacgg cgagtgcgcc
     3301 cgccgtttat aaagttgaag ttagcttgat cgattttgcc actcaggaac ggatgattgc
     3361 atatgcgccg aatggatcgg agttaagcac agatttcgtc ggtgcgatca agattgcgcc
     3421 gcaggtttac gcgtcaccaa cgatcgatca tccgttaaac attcgattgg gcgatcaagt
     3481 cgatctgatc ggttatgcga tcgaaccgat cacgctcgat cgcggcggag tagtccactt
     3541 gcgtttatat tggcgcgccg tgcgtccgcc ggatgccgac tacaccgtgt tcgtgcatct
     3601 gaatgatgct tcgggtcaga cccccgcgca agccgatgcg ccaccgcaat cgggcgcata
     3661 tcccacttcg ttctgggctg ccggcgaagt cgtgatcgac gaccgggata tcgttcttcc
     3721 ccagacggtc agcgccggtc gatatgtgct gggcgttggc ttgtataacc cgatcgatgg
     3781 ctcgcgctta gcggtcgccg gcaatgcttc gaatcagata gatttgccgg atcaactcga
     3841 agtgaaatga ccaaatcacg ctggctgttt ttggcgctga tcctgctggc tttcgcactg
     3901 cgcgtgtatc gccttgaggc gcagagctta tggagcgacg aaggattgtc ggtttaccgc
     3961 gcgcgcttaa ttcttcccga aaatctcacg aatgtgattg tcgtgccacc gaatgtgccg
     4021 acacaggata caaa
//
LOCUS       DASRTJ010000123         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82564,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000123 DASRTJ010000000
VERSION     DASRTJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82564"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFF70_02530"
     CDS             complement(<1..721)
                     /locus_tag="VFF70_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZY43601.1"
                     /translation="MKQNNDHLQAQALFSAFIDHQTTADEKKFVERHLAVCDQDCRAV
                     LAQTRSMISSTRSLPLVKAPRSFVLPKSMARQPARSIFAWYPALRLATSIAVIAFVVV
                     FAGDLFSQRSVMLSANVPSAANAPQLSAAPQPTTVALYAAPPESATIVQAPAAPQTGA
                     GILPTPTTTAMAAADAAAIARSAMTLTDETITATLMTTAEVAMAATPIEPPIADAAPK
                     VVTSAVPAANQVGAIEQTAAPA"
     gene            complement(743..1345)
                     /locus_tag="VFF70_02535"
     CDS             complement(743..1345)
                     /locus_tag="VFF70_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY43602.1"
                     /translation="MDENALIQSARNGDLDSFNSLVLVYQHQVYNLAYRIMGDEAPAA
                     DATQDAFISAYKNLKSFRGGSFKSWLLRIVTNACYDDLRRKKRRPAVSLDELMNNEET
                     DVEYDVPSGEDGPEVIAQRHELAELIQTGITTLPEDQRVTLVLSDVQGLSYEEIAGMT
                     HTNLGTVKSRLSRGRAKLREYLQLHGELLPEAYRLESNRV"
     gene            1490..1574
                     /locus_tag="VFF70_02540"
     tRNA            1490..1574
                     /locus_tag="VFF70_02540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1524..1526,aa:Leu,seq:caa)
     gene            1665..2024
                     /locus_tag="VFF70_02545"
     CDS             1665..2024
                     /locus_tag="VFF70_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43603.1"
                     /translation="MRKILLLVSSLAIGIGLGLFMGWYAWPVNFTETSPADLRSDWKA
                     EVIWMAAQSFAYDGDLELAQTRLAPLGSNDLGLLVLDRANQAIDQKLPRIQVAYLAKL
                     AAALGATSPRLKPYLQP"
     gene            2039..>2431
                     /locus_tag="VFF70_02550"
     CDS             2039..>2431
                     /locus_tag="VFF70_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43604.1"
                     /translation="MPLLIAIGIGSGLAYAWLISPVKFTEASPAQVITEYRHAWLIMA
                     AEAYAEDGNWSRTQERIKSLLDPNLSQTITQLFDQYNAQGPNPIARALALLADRYNAR
                     TAAMLVYLATPIVPPVPIVPPDPIVPPTP"
BASE COUNT          480 a          669 c          716 g          566 t
ORIGIN      
        1 gcgctggtgc ggcggtttgc tcgatcgcgc cgacttgatt cgcggcaggc acagcggatg
       61 tcacgacttt cggtgcggcg tcagcgatcg gtggctcgat cggcgttgcc gccatggcga
      121 cttcggccgt agtcatcaac gtcgcagtga tcgtctcatc tgtcagcgtc atggctgatc
      181 gcgcgatagc ggccgcatcc gcggcggcca tcgcagtggt ggtgggtgtc ggcagaatgc
      241 ccgctccagt ttgaggcgcg gccggcgctt gcacgatggt ggctgactcg ggcggcgcag
      301 catacagcgc caccgtcgtt ggctggggag cggctgagag ttgcggcgcg ttggcggccg
      361 acggcacatt ggcgctgagc ataactgatc gttgagaaaa gagatcgccg gcaaaaacca
      421 ctacgaaggc aatgacggcg atcgaggtgg cgagccgcag tgccggatac caagcgaaga
      481 tcgatcgggc tggctgacgg gccatcgatt tgggcagcac gaatgatcgc ggcgctttga
      541 ccagcggcag cgatcgggtc gagctgatca tcgatcgggt ttgcgctaaa accgcgcggc
      601 agtcttgatc gcatacagcc aaatgccgct cgacgaattt cttttcgtca gcggtggttt
      661 gatgatcgat aaaggcggaa aataatgcct gcgcctgaag gtgatcgttg ttttgcttca
      721 tctttgaaat tccacgtccc gattaaactc ggttgctttc tagacgatag gcctcgggca
      781 aaagttcccc gtgcaattgc aggtattcgc gcagtttggc gcgtccccgg ctgagccgcg
      841 atttgaccgt gccgagattg gtatgggtca tccccgcgat ttcttcataa cttaaaccct
      901 gcacatcgct gagcaccaat gtgacgcgct gatcttcggg cagggtcgta atgcctgtct
      961 ggatcagctc ggccagttcg tgccgctgcg cgatcacttc cggcccgtct tcgcccgacg
     1021 gtacatcgta ctcgacgtcg gtctcttcat tattcatcaa ttcatccagt gagacggcgg
     1081 ggcggcgttt cttgcggcgg agatcgtcat agcaagcatt ggttacaatg cgcagcagcc
     1141 aactcttgaa cgatccgccg cggaacgatt taagattttt gtaagcggag ataaaagcgt
     1201 cctgcgtggc atcggccgcc ggggcttcat cgcccatgat gcgataggcg aggttgtaga
     1261 cttgatgctg atagaccagc accagtgagt tgaaggaatc cagatcgcca tttcgcgcgg
     1321 attgaatgag ggcgttttcg tccatagaaa atggtaatca accacggatg acccggctgc
     1381 caggcagtga tgatgagctg gacaaaataa tccgcgtcat ctaggtcagc tgtgattcaa
     1441 gtgatcttga cgaatgaatc agcctcgact atacttacgg catcttcagg ccggagtggc
     1501 ggaactggca gacgcgcacg actcaaaatc gtgtatcctt aacggatatg tgggttcgat
     1561 tcccaccttc ggcattactc accgtctttc gcgccgatta taccagacta cccatgacgg
     1621 gcctgatttc gatcgagccc gttttgtttt gccccgggtg atttatgcgt aaaattctac
     1681 tgctcgttag ctcactggcg attggcatcg gtcttggtct gttcatgggc tggtatgcgt
     1741 ggccggtcaa cttcactgaa acttctccag ccgatctgcg atccgattgg aaagcggaag
     1801 tgatctggat ggccgcccag tcctttgcct acgatggcga tctggagctg gcccagaccc
     1861 gattggctcc gctcggctcc aacgatctgg gtttacttgt actcgatcgc gctaaccagg
     1921 cgatcgatca gaagctgccc aggatccaag ttgcctattt ggcaaaattg gctgcggcgt
     1981 taggcgccac ctcaccgcgg ctcaaacctt acttgcagcc atgaaactta aattgcttat
     2041 gccgttgctc attgcgatcg gaattggctc gggactcgcc tatgcgtggc tgatcagtcc
     2101 ggtcaaattt accgaggctt cgcccgcgca ggtcattacc gagtatcgcc acgcgtggtt
     2161 gatcatggcg gcggaggctt atgccgaaga tggcaattgg agtcggacgc aggaacgcat
     2221 caagagtttg ctggatccga atctgtctca gaccatcact cagttgttcg atcaatacaa
     2281 cgcgcaaggt ccgaatccga tcgcgcgcgc gttggcgctg ttggccgatc gatataacgc
     2341 gcgcactgcg gcgatgttgg tttatctggc aaccccgatc gtgcctcctg tcccgatcgt
     2401 tccgcccgac ccgatcgttc ctccgacccc g
//
LOCUS       DASRTJ010000124         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_45107,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000124 DASRTJ010000000
VERSION     DASRTJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45107"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFF70_02555"
     CDS             complement(<1..778)
                     /locus_tag="VFF70_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZY43605.1"
                     /translation="MTSIGKRVLTPEIALLGLIRTPDTSARRILDKIAIERGFKIDDL
                     DRDTEAQAKLRDGRAADFVFVLDNGGKVQLSDEMLQSIDEALSIAQAADEVWIGSEHL
                     LSALSQRGVSTAGLLQARGITPTALTSYLSDRSLARRMTTSDWVAEARSGEITPMYFR
                     ENLLRELMSLLSLSGPRHVLLIGPQGSGRRSIAYSLALLIAEGKGPTGIKSMIEINEP
                     AVLDNPIEALRSGVKQAASGILFLPNVSRFIGRGVNPQVEK"
     gene            complement(1335..1745)
                     /locus_tag="VFF70_02560"
     CDS             complement(1335..1745)
                     /locus_tag="VFF70_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43606.1"
                     /translation="MTGGATARTTSGDIAVTNADKNERVVGTVNSNTTSSGKRLINLR
                     EPSANGNVSFSESLVKNSAANRVAVDNGNASLTLPGDAQFGIDALTSNGTLASDFTFQ
                     GDTSPTSVKGTLGASPLLGNTIRVKNGTITVKKD"
     gene            1901..3061
                     /locus_tag="VFF70_02565"
     CDS             1901..3061
                     /locus_tag="VFF70_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43607.1"
                     /translation="MTTFDLILILLLAIAIYFYARDHTAIQTLRRSIDRRELDRSSST
                     DRLGQLEARNAALGDAPDHPLFVVNDQGVIEHLNSHAIDLLEANAIGRTLIEVTRSHA
                     IDALIQATLTSHHTSEQLLSWNDRSFQARAVPIQSGGAVLVLEDRTAQQRADWARRDF
                     VANVSHELRTPLASIRLLIETLLSGAQDDPSISTRMLTQVVTQVDALTQLAQELLDLS
                     MIESGQMPMQRSRENMRDVIEEQLARFEPQAQPKKIVLANSAPDEVRVEIDRTMIGRV
                     LGNLIHNALKFTPPNGVITIGASSNDRETVMSVSDTGEGIAPEELPRIFERFYKVDRA
                     RGASSGTGLGLSIARHVIEAHGGRIWAESKLGKGTTFYFSIPNDKARMTSEK"
     gene            complement(3124..3807)
                     /locus_tag="VFF70_02570"
     CDS             complement(3124..3807)
                     /locus_tag="VFF70_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43608.1"
                     /translation="MTEPQQKLLIVEDDQTLRETLSYNLTREGYAVTASGDGGEGLEL
                     ARDNLFDLIVLDVMLPTLDGLSIVRILRKEQRTPIILLTARSGEVDRIMGLETGADDY
                     VVKPFSLGEFLARVRAVLRRATPETATRVESGDLSLDLIGRRAYRAAEELRLSHKEFD
                     LLAALIQNSGTALSRDRLLQQVWGYDYPGDTRTVDVHVRWLREKIESDPAEPQRIVTV
                     RGVGYRFEK"
BASE COUNT          923 a         1142 c         1013 g          891 t
ORIGIN      
        1 tcttctcgac ttgcggattt actccgcgtc cgatgaagcg gctgacattc ggcaaaaata
       61 aaatcccact cgcggcttgc ttcacacccg atcgcaatgc ctcgatcgga ttgtccagca
      121 ctgccggttc gttgatctcg atcattgact tgatcccggt cggtcctttg ccctcagcga
      181 tcagcagcgc cagactgtac gcgatcgatc gacgcccgct gccctgcggg ccgatcagca
      241 gcacgtgccg aggaccgctt agcgaaagca gactcatcaa ctcgcgcagc agattttccc
      301 gaaaatacat cggggtgatc tcgcctgatc gggcttcagc cacccaatcg ctcgttgtca
      361 ttcggcgcgc cagcgatcga tccgacaaat aactcgtcaa tgccgttggc gtaatgccgc
      421 gcgcttgtaa taaaccggct gtgctcacgc cgcgctgcga caacgcgctc aacaaatgct
      481 cgctgccgat ccacacttca tccgccgcct gcgcgatcga caacgcctca tcgatcgact
      541 gcagcatctc atcggatagc tgcactttgc cgccgttgtc caacacgaat acaaaatcag
      601 ccgcgcggcc atcgcgcaat ttcgcttgag cctccgtgtc tcgatcgaga tcatcgatct
      661 tgaaaccgcg ctcgatcgcg atcttatcga gaatacgccg cgctgacgta tcgggcgtac
      721 ggatcaagcc cagcagcgcg atctccggcg tcagcactcg cttgccgatc gacgtcatga
      781 tctttgccga agcgttcaga atgagtgaag catgtttgga gagtagattt ggattgagcg
      841 tgcccatgtt ttagaagttg gaggttggac gttggaagat tgatcgagcc ttgaaatcag
      901 aaatgtgctg gttcgagata aaatccactt acgataagcg atttgaagca cgctgcgtga
      961 cagagccaca cttgccagag atctttcgtt gtcgaaaccg ttacatgaca aggatcgatc
     1021 tcattagggg taatcgattt gccacaaaaa caacgaggcc cccattcgac gacttgttgc
     1081 cgcttatcat ctgacataga cattttatac tccatcaacc gcgtccatac gtttgaccgt
     1141 caacgagtct cttgcacgat aagagtaaca cgaaagacaa gaagattcaa agctaagatg
     1201 atcttgctcc tctctcccaa cgacgcactt gcttttcgtc gttggaagcg gggctggagg
     1261 ttgaggcaac agaaaggccg aagatcgaaa catcgatcct cagccttcaa tcagaaatca
     1321 aagagcagag attcttaatc tttcttcacc gtgatcgtcc cgttcttcac gcggattgta
     1381 ttaccgagca gcggcgacgc acccaacgtg cctttcaccg aagtgggcga cgtgtcccct
     1441 tggaaagtaa aatcgctggc cagcgtgcca ttcgaagtca acgcatcaat gccgaattgt
     1501 gcatcgcccg gcagcgtcag gctcgcattg ccattatcca cagccactcg attggctgcg
     1561 ctgttcttaa ccagcgattc gctgaacgac acgtttccat ttgccgaagg ctcgcgcaga
     1621 ttgatcaaac gctttccact cgacgtcgta ttactgttga cggtgcctac cacccgctca
     1681 ttcttgtccg cattcgtcac cgcaatgtcg ccgctcgtcg tgcgcgctgt cgcgccgccg
     1741 gtcatgccat cgccataaaa ccaatcggcg tcaatttcag acttacatct ttcatggtat
     1801 cctcttagta gatatacaga gcgcccagat tgtttcatgg gatatttgaa attcatccgg
     1861 tacattaggt tcaatcctgt ttagtgcttg acgcatcacg atgaccactt tcgatctcat
     1921 tctgattctc ttgctggcaa tcgcgatcta tttttatgcg cgcgatcata ccgcgatcca
     1981 aacgctgcgc cgctcgatcg atcgacgcga actcgatcga tccagctcaa cagatcggtt
     2041 gggtcagctc gaagcgcgca atgccgcatt gggcgatgca cccgatcatc cgctgttcgt
     2101 cgtcaatgat cagggcgtga tcgaacatct caactcccac gcgatcgatc tactggaggc
     2161 caacgcgatc ggccgcaccc tgattgaagt cacgcgctcg catgcgatcg atgcgctcat
     2221 tcaagctacg ctcacttcac atcacaccag cgagcagctg ctcagctgga acgatcgatc
     2281 gtttcaggcg cgggcagtgc caattcaaag cggcggcgcc gtgttggtct tggaagatcg
     2341 tacggcgcag cagcgcgcgg actgggcgcg gcgcgatttc gtcgcgaacg tctcgcacga
     2401 actgcgcaca ccgctagctt ccatccgctt gttgatcgaa acgctgttga gcggtgcgca
     2461 agatgatccg tccatatcaa cgcgcatgct gacccaagtc gtcacgcaag tggatgcact
     2521 gacgcagttg gcgcaagagt tgctcgactt gtcgatgatc gaatcggggc agatgccgat
     2581 gcagcgctca cgcgaaaaca tgcgtgatgt gattgaggaa cagctcgcgc gtttcgaacc
     2641 gcaggcgcag ccgaaaaaga tcgtgctcgc caattccgca ccggatgagg tgcgcgtcga
     2701 gatcgatcgc accatgatcg gccgcgtgtt gggcaacttg attcacaatg cgctgaagtt
     2761 tacgccgccg aatggcgtca tcacgatcgg ggccagctca aacgatcgag aaacagtgat
     2821 gtccgtcagt gacacgggcg aagggatcgc cccggaagaa ctgccgcgta tcttcgagcg
     2881 cttctacaaa gtcgatcggg cgcgcggggc gagcagcggt acgggcttgg gcttatcgat
     2941 cgcgcggcat gtgatcgaag cgcatggtgg ccgcatttgg gcggagagca aacttggcaa
     3001 aggtacgacg ttctattttt caattccaaa tgacaaggcg agaatgacaa gtgaaaaatg
     3061 acaaatgaac aaaggcatcc gttttcattt gtcattggac atttgctcat ttgtcatttc
     3121 aattcatttt tcaaatcgat aaccgacgcc gcgcacagta acaatcctct gcggttcagc
     3181 cggatcactt tcgatcttct ctcgcagcca gcgcacgtgc acatccaccg tgcgcgtatc
     3241 gccgggataa tcgtagcccc acacttgttg caacagccga tcgcggctca gcgccgtgcc
     3301 ggaattttgg attaacgccg cgagcaaatc aaactctttg tgactgagcc gcagttcttc
     3361 cgcggcgcga tacgcccgcc ggccgatcag atcgagcgac agatcgccgg actcaacgcg
     3421 cgtcgcggtt tccggcgtcg cacggcgcaa aactgctctc acccttgcca ggaattcacc
     3481 cagactgaac ggcttgacca cgtaatcatc cgcgccagtt tccaggccca tgatgcgatc
     3541 gacctcgccg ctgcgggccg tcaacaaaat gatcggtgtg cgctgctctt tgcgcagaat
     3601 ccgcacgatc gataaaccgt ccagcgtcgg cagcatcaca tccagcacaa tcagatcgaa
     3661 tagattgtcg cgcgccaatt ccagcccttc gccgccgtcg ccggacgcag tcacggcata
     3721 gccttcgcgt gtcaagttat aactcaacgt ttcgcgcaga gtttgatcgt cttcgacaat
     3781 gaggagtttt tgttgaggtt cagtcattat tgacagtata acccattcag cgcccggctc
     3841 gcaagtcaaa tcatgttaag gaactttaac gtcagcttga cgtgaacttg aacgagtgag
     3901 gttacactgt gaatgtaaaa atggaagaag aatacagaaa ggagaacgat atggctcaag
     3961 aaattgaaa
//
LOCUS       DASRTJ010000125         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7277,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000125 DASRTJ010000000
VERSION     DASRTJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7277"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFF70_02575"
     CDS             <1..579
                     /locus_tag="VFF70_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY43609.1"
                     /translation="AGKWQKSKQFCLLTHELHDINRSTLGIIGHGSIGKAMQRLGESV
                     GMRVLISEHKQASKIREGRTSFEDTLRESDVVSLHCPLTDETRDMLGAAEFAMMKSNA
                     LLINTARGALVEDAALIEALQNGSIAGAGYDALREEPPREGSPLLDLNLPNFIVTPHV
                     AWASNEAVQALADQVIDNIEAFIAGTPRNLLT"
     gene            718..1845
                     /locus_tag="VFF70_02580"
     CDS             718..1845
                     /locus_tag="VFF70_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HZY43610.1"
                     /translation="MPWQECSTMSLRQEFLGLAAQPGSNFSQLCRRFGISRKTGYKWW
                     RRYRETGVSGLADQSRRPQHSPRHSQPALEQQVLAVRDQYGWGARKIKTCLERAGQGP
                     LAKSTVHKILRRHERLTSSADQVSRPYQRFEKERANQLWQMDFKGHYQLGNQERCHPL
                     TVLDDHSRYSLCLQACRNEQTGTVQQQLIMAFRRYGLPERMLMDNGSPWGHDHEHQYT
                     PLTVWLMHLGVGVSHSRPYHPQTQGKDERFHRSLKVEVLAQRVFADFERMQVRFDEWR
                     YCYNHVRPHEALAMAVPASRYQPSQRSFPEQLPPLEYGATDQVRKVQTDGKISFRNRE
                     FRLGKAFRGYPVALRPTTEDGVYEVYFATHRISQIDLHRPK"
BASE COUNT          482 a          540 c          607 g          442 t
ORIGIN      
        1 gccgggaagt ggcaaaagtc aaaacagttc tgtctgctga cccacgagct gcacgacatc
       61 aaccggagta cgctgggcat tatcggccac ggctcgatcg ggaaagcgat gcagcggctt
      121 ggcgaatctg tcggcatgcg ggtgctgatt tcagaacaca agcaagcttc aaagatccgt
      181 gaagggcgca cgtcattcga agatactctg cgggagagcg acgtggtcag tttgcattgt
      241 cctttgactg atgaaacccg ggacatgttg ggcgcggcag agtttgccat gatgaagtcc
      301 aacgcgctgc tgattaatac cgcgcgcggc gctttggttg aggacgcggc cctgattgaa
      361 gccctgcaaa acggatcgat cgccggcgcc ggctatgacg cgctgcgcga agagccaccg
      421 cgcgagggca gtccgctgct tgatctaaac ttgccgaatt tcattgtcac gccccacgta
      481 gcctgggcca gcaacgaagc ggtccaagct ctcgccgatc aggtgatcga caacatcgaa
      541 gccttcatcg ccggcacgcc tcgaaatttg ttgacctgat taattgttta gaggccgcac
      601 ttgtgcctta ctccctctcc ctttgggagt gtacgtaccg gagacatagg taacaggtgt
      661 ccacagacat gggtaacaca ttcgacaatg gcatgaatac ctttttagga gggaaacatg
      721 ccttggcagg agtgttcgac catgagtctg cgacaagagt ttttggggct ggccgctcaa
      781 cccggaagca atttcagcca gttgtgtcgg cggtttggca tcagccgcaa gactggctac
      841 aaatggtggc gaagataccg agagactggc gttagcgggt tggccgatca atcgcggcga
      901 ccccaacact cgccgcgaca tagccagcca gcgctggaac aacaagtgct ggcagttcgc
      961 gatcaatatg gctggggcgc acgcaagatc aagacgtgcc tggagcgagc tgggcaaggc
     1021 cctttggcta agagcacggt ccataagatt ttgcggcggc atgagcggtt gaccagctcg
     1081 gctgatcaag tgagccgtcc atatcaacga tttgaaaaag agcgagcgaa ccagctgtgg
     1141 caaatggact ttaagggtca ctatcagttg ggtaaccagg agcgctgtca tccgctgacc
     1201 gtgctcgacg atcactctcg ttattcgttg tgcttgcagg cctgccgcaa tgagcagacc
     1261 gggaccgtgc aacagcaact gattatggct tttcgccgtt atggcttgcc tgagcggatg
     1321 ctgatggaca atggatcgcc ctggggtcat gaccacgagc atcagtacac gccgttgacg
     1381 gtttggctga tgcatctggg cgtgggcgtc agccacagtc ggccttatca tccgcagact
     1441 cagggcaaag atgaacgctt tcaccgcagt ttgaaagtcg aagtgttggc ccagcgagtg
     1501 ttcgctgact ttgagcggat gcaggttcgt tttgacgaat ggcgatattg ttacaatcac
     1561 gtgcgaccgc atgaagcgtt ggcgatggcg gtgccggcca gccgttatca accgagtcag
     1621 cgatcttttc cggagcagtt gccgccgctc gagtacgggg caacggatca ggtgcggaag
     1681 gtgcaaactg atgggaagat atctttccgc aaccgcgaat ttcgcctcgg caaagcgttc
     1741 cgcggttacc cagtggcgct ccgacccacg actgaggatg gagtttacga ggtttacttt
     1801 gctactcatc gaatttcgca aatcgatctg catcggccaa agtaatcgac ctaggactgt
     1861 tgcttgataa ccagttcggt ataaaaccga acgatgaaaa gtgttaccca tgtctgtgga
     1921 catgtgttac ccatgtctcc ggtctataca ctccccagag gaaggggaag aaaccagagt
     1981 ctgattggcc actggatagg atctgcgaat attacgtcgt caggcaacga acactcagcc
     2041 gggcgagcat caaacatccg cactcaacga a
//
LOCUS       DASRTJ010000126         6735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82707,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000126 DASRTJ010000000
VERSION     DASRTJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82707"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1324)
                     /locus_tag="VFF70_02585"
     CDS             complement(191..1324)
                     /locus_tag="VFF70_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZY43611.1"
                     /translation="MSTYTSTTWANPAEPETKIPQLQRLLWDVFSVTETRVREAPDHV
                     VDFRGRITQAPSEVHMTINSRFNDLGYTAFLYRDNNADVIQAVEGVVKPKPAQVWINL
                     LLLALTIITTLMAGAVIAGVRVGSLEIFARQPELLLRGIPFGASLLLILGIHEMGHYV
                     AGRLHKAAVTLPYFIPVPPFGALPLGTLGAFIQLRSPIQNRRALFDIGLAGPIAGLVV
                     AIGVFVYGSTISRVIHTASGITLGRSIFTQFVIGLIHPDAVRSGFGVETNPFLLAGWF
                     GLFVTVINLLPIGQLDGGHILYAVLGRYARLFGIATIVGLLALGYFTQSPTWTLWGFL
                     GLVFGINHAPPLDDITPLDPPRKLIAIITFIIFVLIFVPTPFR"
     gene            complement(1383..2579)
                     /locus_tag="VFF70_02590"
     CDS             complement(1383..2579)
                     /locus_tag="VFF70_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZY43612.1"
                     /translation="MEQSQNNVFSPPSTLETVAHLRSVLSQVMHIETHVIHVPPVDVI
                     EFVGQLTVPSEEAFDLIYMRFTDLGYTPMLTRRDDGKEIVVAKHGIVEPTPNRNSTNL
                     ILLILTIISTVLVGTTLYGGEALVKFIPANADQGDIFAIFGIVLSHPQLLLSGLPYAL
                     TLLGILGVHEMGHYVMARVHKAAVTLPFFIPMPIGLGTMGAVIRLKSPIKNRKQLFDI
                     GIAGPLAGLAIAIPLLIVGLVMSPVEFVGKPIPGGQEGNSILYFLLKLIIKGRALPGG
                     GYDVILSTIAFPAWFGLIVTMINLLPIGQLDGGHIIYSIFGRTQWRVASIAIFGLIGL
                     GAYLAYTTGEIINVWLLWVLLAQVFGLRHPPPLDDITPLDRRRKLIGYATIVIFILIF
                     TPIPFS"
     gene            complement(2724..4598)
                     /locus_tag="VFF70_02595"
     CDS             complement(2724..4598)
                     /locus_tag="VFF70_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZY43613.1"
                     /translation="MLRSPEKKSNENNPLGGMDQFRRLFAFVKPYRKRLAIAISAIII
                     GSILGLAGPYTLQFLIDAVFRQSNATLLNQITLALLIIFGSQAIFYFIRGYLLQFIGE
                     RVMADLRLKLFEHLESLSLSFFNERRTGELVSRLTNDVSTVRSVVTSDISTALSQALT
                     FIGALILIVITDWRLTLFMFALIPVVMLFGIMFGRRLRKLSGAVQDQLAGATTVLEES
                     IGGVRVVQSFAREDYEIGRFRDAIESTFKLAMNRIRISAVFGPLVSFLGFGAVVSIFW
                     FGGHEVLAGRLTAGQLFMFLILTMTIAGSIGSFSGLWTGLQEALGATKRLFEILDTKP
                     DIADAPNARSLPRMQGMIEFDRVSFAYRDNTGTAILSDVSIKVKPGEIFALVGPSGAG
                     KTTLVNLIPRFFDPISGRICIDGIDIREVQVKSLREQIGLVPQETLLFGGTVRENILY
                     GKLDATEAEMIDAARAANAHDFVLQLPKSYDTIVGERGVKLSGGQRQRIAIARAILKD
                     PRILLLDEATSSLDSESEGLVQEALERLMQNRTSIVIAHRLSTVHHADRIAVLDEGKL
                     VELGTHPELMALDGLYARLYKLQFKIDEPIVPHTENEPSVSEKPKRRSTNILAALAGS
                     "
     gene            4964..5371
                     /locus_tag="VFF70_02600"
     CDS             4964..5371
                     /locus_tag="VFF70_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43614.1"
                     /translation="MLTLRLQKGTVMMLPDPLPTDPDELEKLYQDYLTSDGFDDAEFQ
                     RLMDARLTSWGIDPHKMSADQLLGAMTESMNKMLLNLHAAMGEAPDEESAKQLTEIIN
                     MAEQLRTDIGKAMEDTGGEADQLTSGDAPENDK"
     gene            complement(5466..6449)
                     /locus_tag="VFF70_02605"
     CDS             complement(5466..6449)
                     /locus_tag="VFF70_02605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY43615.1"
                     /translation="MQYRVLGRTGWKVSALSFGAWAIGGTWGPVKDAESLAALHRAIE
                     LGVNFFDTADVYGDGHSERLLAQLRREHKETIYIATKAGRRLDQQTAAGYNRANLTVF
                     VERSLKNLDTEALDLLQLHCPPTQVYYMPEVFGILDDLIAAGKVRYYGVSVEKVEEAL
                     KAIEFPNVQSVQLIFNMFRLRPIELLFDQVLKRKVGILARVPLASGLLTGKLKPGNTF
                     TSDDHRAYNRAGESFDRGETFSGVDYDIGLQAVEELKAICPTGMSMTQFALRWVLMFE
                     AVTCAIPGAKRPSQAEENFAAADLPPLSDTTLAQVRSIYDRHIRERVHAYW"
     gene            complement(6507..>6735)
                     /locus_tag="VFF70_02610"
     CDS             complement(6507..>6735)
                     /locus_tag="VFF70_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HZY43616.1"
                     /translation="KFVLGNKVMVGSVNANRVYFEMGVQDMALAEQYWPGWLKRFLTH
                     PLKGLDQYQEMIRLLTEEKKAIKVYIEVESE"
BASE COUNT         1696 a         1796 c         1757 g         1486 t
ORIGIN      
        1 gcaccgaaaa aacatatgta gactgggttt gctcgaaggc ggtctgtccc acagggaggc
       61 ttcgcactga ccgccgtgtt ttgcagtgtg gccgcagggt cacagaccct gcgggagtaa
      121 caacgacaca gcccgatcga aattcgatcg ggctgttgat ttactcggca ttcattcgca
      181 ccaagttcaa ttacctaaac ggcgttggta cgaagatcag cacgaagata atgaaggtaa
      241 tgatggcgat caacttgcgc ggcggatcga gcggtgtgat gtcatcgagt ggtggcgcgt
      301 gattgatgcc gaagaccagc cccaagaatc cccacagcgt ccacgtcggc gattgtgtga
      361 agtagccgag cgctaataaa ccaacgatcg tggcaatgcc gaacaatcgc gcgtagcggc
      421 ccagcaccgc gtaaagaatg tgcccgccgt cgagttggcc gatcgggagc aaattgatca
      481 ccgttacgaa taatccgaac cagcccgcca acaaaaatgg attcgtttcc acgccgaaac
      541 ccgatcgaac tgcgtcggga tgaatcaggc caatgacgaa ttgcgtgaag atcgatcggc
      601 cgagggtgat tccgctcgcc gtatgaatga cgcgtgagat ggtcgacccg tagacgaaca
      661 caccgatcgc aacaaccagc ccggcgatcg gtccggccaa gccaatatca aacaatgcac
      721 gtcgattctg aatcggtgat cgcaactgaa tgaatgcgcc gagcgtgccc agcggcagtg
      781 cgccaaacgg cggcacaggt atgaagtacg gcagcgttac cgccgctttg tgaagtcgtc
      841 cagccacgta atgccccatc tcgtgaatgc ccaagatcag cagcagcgat gcgccaaacg
      901 gaatgccgcg cagcaatagc tcaggttgtc gcgcgaaaat ttccagcgag ccaacgcgca
      961 cgccggcaat gaccgcgccc gccatcaatg tggtgatgat cgtcaaggcg agcagcagca
     1021 gattgatcca cacctgcgct ggtttcggct tgacaacacc ttcaacggct tggattacat
     1081 cagcattgtt gtcgcggtac agaaaggccg tgtagcccag atcgttgaag cggctattga
     1141 tcgtcatatg cacttcgctc ggtgcttgcg taatccgtcc gcgaaagtcc actacgtgat
     1201 caggcgcttc gcgcacgcgc gtttctgtta cgctgaatac atcccacaat aagcgctgca
     1261 attgtgggat tttggtttca ggctcagctg gattggccca agttgttgaa gtgtaagtgc
     1321 tcatgttgat gttggaagtt ggctgaacga tctaatttca aattccgaac atccagtttc
     1381 aatcacgaaa acggtatcgg tgtaaagatc aaaatgaaaa tgacgatcgt tgcatacccg
     1441 attagcttgc ggcgccgatc gagcggcgtg atgtcgtcga gcggcggcgg atggcgcagg
     1501 ccaaagactt gcgccaacaa aacccacagc agccacacat tgataatttc gcccgttgta
     1561 taagcgagat aagcgcccaa accgatcagc ccgaagatcg cgatcgaggc tacgcgccat
     1621 tgcgtgcgtc cgaagatcga atagatgatg tgcccgccgt caagttggcc gatcggcagc
     1681 aggttgatca tcgtaacgat caagccgaac cacgccggaa atgcgatcgt gctcaagatc
     1741 acatcatacc caccgccggg cagcgcgcgt cccttgatga tcaacttcaa caggaagtat
     1801 aagatcgaat taccttcttg tccgccgggg atcggcttgc cgacaaattc gacgggcgac
     1861 atgaccaatc caacgatcaa cagcggaatt gcaatcgcga gcccggcgag cggtccagcg
     1921 atgccgatat cgaacaattg tttgcgattc ttaatcggcg acttcaaccg aatcactgcg
     1981 cccatcgtcc ctaaaccgat cggcatcgga ataaaaaaag gcagagtcac tgcggctttg
     2041 tggacgcgag ccatgacata gtgtcccatt tcatgcacac ctaaaatacc tagtagtgtt
     2101 aaggcatagg gtaacccgct gagcaaaagt tgcggatggc tcagcacgat tccaaagatt
     2161 gcaaatatat caccctgatc ggcattggct gggatgaatt taactagagc ctcgccgcca
     2221 tacaatgttg tcccgaccaa gaccgtgctg atgatggtca agatcagcag gatcaaatta
     2281 gtactatttc gattgggcgt aggttcaacg atgccgtgct tggcgacgac gatctctttg
     2341 ccgtcatcgc ggcgagtgag catcggagtg tagcccagat cggtgaagcg catgtagatc
     2401 agatcgaacg cttcttccga gggtaccgtc agctgcccga cgaattcgat cacgtcgaca
     2461 ggcggcacgt gaatgacgtg cgtctcgatg tgcatcactt gactgagcac cgatcgcaag
     2521 tgcgcaacgg tttcaagagt tgatggtggt gagaagacgt tattctgtga ttgttccatt
     2581 aaatcctcat cgtcattacg tcatttcaat gaagccgcag gccgacgcgg caatccacgg
     2641 atcgtttttt aagattgctc cgtggcacat ccctcgaggc tgcgccagcg agggacgccg
     2701 cttgcaatgc aggtgatggg tgattacgat cccgccaatg ccgctaaaat attggtcgat
     2761 ctgcgtttgg gtttttcact aaccgatggc tcattctcgg tgtgcggcac gatcggctca
     2821 tcgatcttga actgcagctt atataacctc gcgtataaac catccagcgc catcaactca
     2881 ggatgcgtgc ccagttcgac caatttacct tcatcaagca ccgcaatccg atcggcatga
     2941 tgcacggtcg ataatctatg cgcgatcacg atgctggtcc gattctgcat caatctttca
     3001 agcgcctctt gcactaagcc ttcgctttcg ctatcgagcg atgaagtggc ttcgtcgagc
     3061 agcagaatgc gcgggtcttt caagatcgcc cgcgcgatgg cgatgcgttg acgttgcccg
     3121 ccgcttaatt tcacgccgcg ttcgccgaca atcgtatcat aactcttggg aagttgaagc
     3181 acgaagtcgt gggcattggc cgcgcgggcc gcatcgatca tttcagcttc agtggcatcg
     3241 agtttgccat acaaaatatt ttcgcgcacc gtcccgccga ataacaaagt ttcctgcggc
     3301 accaggccaa tttgttcgcg cagacttttc acctgcactt cacgaatatc gatgccatcg
     3361 atacagatgc ggcccgagat cggatcgaaa aagcgcggga tcaaattaac cagcgtcgtc
     3421 ttgccggcgc cactcggtcc gacgagcgcg aatatttcgc ccggcttcac tttgattgaa
     3481 acatcagaca agattgcggt gccggtgtta tcgcgatagg caaatgaaac ccgatcgaat
     3541 tcgatcatgc cttgcatgcg cgggagcgat cgagcgttgg gcgcgtcagc gatatcgggc
     3601 ttggtatcca gaatttcaaa taagcgtttc gtcgcaccga gtgcttcctg caagcccgtc
     3661 cacaaaccgc tgaacgatcc gatcgatccg gcaatcgtca tcgtcaagat caagaacatg
     3721 aagagctgtc ctgcagttaa gcgtccggct aaaacttcgt ggcctccgaa ccagaagatc
     3781 gagacgactg cgccgaaacc gagaaacgac acaagcgggc caaagacggc cgagatgcga
     3841 atacggttca tcgccaattt gaatgtcgat tcgatagcgt cacgaaagcg gccgatctca
     3901 taatcttcac gcgcgaacga ttgcacgacg cgcacgccgc cgatcgattc ttccagcacg
     3961 gtcgttgcgc ccgccagttg atcttgcacc gcgcccgata gtttgcgcaa acgccgaccg
     4021 aacatgatcc cgaacaacat cacgaccgga atcagtgcga acatgaacag cgttaagcgc
     4081 caatcggtga tgacgattaa gatcaacgcg ccgataaaag taagggcttg cgataaggct
     4141 gtcgaaatgt cgctcgtcac cacgctgcgt acggtcgaaa catcgttagt caagcggctg
     4201 actaattcgc cagttctgcg ttcgttgaag aaactcagcg acaaactttc taaatgttcg
     4261 aataacttta gccgaagatc ggccatcacc cgttcgccga tgaattgcag caagtaaccg
     4321 cgaatgaaat agaaaatagc ttgtgaccca aaaatgatca gcaaggcaag cgtgatctga
     4381 ttgagcagcg ttgcattgct ctggcgaaag accgcgtcga tcaggaattg cagcgtgtat
     4441 ggccccgcca agccgaggat actgccgatg atgatcgcgc tgatcgcaat cgccaatcgc
     4501 ttgcgatagg gcttgacgaa agcgaacaag cggcgaaatt gatccatccc gccaagcgga
     4561 ttattctcat tcgatttctt ttcgggtgaa cgcagcatgg taactcagaa gataagcggc
     4621 tcgggcttcc atgcccgagt caattgagaa cagaatcgag gctgcagccg gtgtgtgccg
     4681 cgaacatccg gctgaagcct cgattacaat tccaggaaag catgggtcaa gtatatcctg
     4741 ccgatcaatc gccgtcaagg aattgcttcg atggtcgatc tgatttattc agcgcagtaa
     4801 accgacgcca tgtcgagtgc gccgatgttt ttgttactta caacatcagt tagccgggga
     4861 agatattacc tgccgcgaaa aagatcgcga ttcgaattgc cagatggagg aaatcgatta
     4921 ttgcaggccg atcgaatttg ccgaacgagt tttcagcagt ataatgctca cacttcgatt
     4981 acaaaagggc actgttatga tgctacccga tccgctgccg accgatccag atgagcttga
     5041 gaagttgtat caagactatt tgacttcgga tggtttcgac gatgccgagt tccagcgatt
     5101 aatggatgcg cggctgacgt cgtggggaat cgatccgcat aagatgtcgg ccgatcaatt
     5161 actgggcgcg atgaccgaat cgatgaacaa aatgctgctc aatctgcatg cggcgatggg
     5221 cgaagcgccc gatgaagaat cggccaagca gttgacggag atcatcaaca tggccgagca
     5281 gctgcgtact gatatcggta aggcgatgga ggatacaggc ggggaagcgg atcaattaac
     5341 atccggcgat gcgcctgaaa atgacaaatg aaaaatacca aatgacaaaa tgagcgccga
     5401 ggaaggtcgg cgcgtttatt ttcaaccatt acgtatcact ggttacgctt cagcatggct
     5461 caagtttacc agtaagcgtg tactcgttcg cggatgtggc ggtcgtaaat tgagcgtacc
     5521 tgcgccagcg tggtgtcaga gagtggaggt agatctgcag cggcgaagtt ctcctcagct
     5581 tgcgacggac gctttgcgcc gggaatagcg caggtcacag cctcaaacat caatacccag
     5641 cgcaaggcaa actgcgtcat actcattcct gtcgggcaga tagcctttag ttcttcgacc
     5701 gcctgcaacc caatatcgta atcgacgcct gagaaagttt cgccgcgatc aaatgactca
     5761 ccagcgcgat tataggctcg atgatcatcc gatgtgaaag tgttgcctgg ctttagcttg
     5821 cccgtgagca gcccggaggc cagcggcacg cgcgccagaa tacccacttt acgtttcagc
     5881 acctgatcaa acagaagttc aattggacgc agacggaaca tgttaaaaat gagttgcacg
     5941 ctttgcacgt tggggaactc tatggccttt agcgcttcct ccaccttttc cacactgacc
     6001 ccgtagtagc ggactttgcc ggctgcgatc aaatcatcga gtatgccaaa gacctccggc
     6061 atatagtaga cttgagtggg tgggcagtgg agttgaagca aatcgagcgc ttctgtatcc
     6121 agattcttga gactgcgctc cacaaagact gtcaggtttg cacggttata gcctgctgcg
     6181 gtttgttgat ccaaccttcg tccagccttc gtggcaatgt agatggtttc tttgtgctcg
     6241 cgacgcaatt gcgccagcaa gcgttcgctg tgaccatcgc cataaacgtc ggcggtatca
     6301 aagaaattga cgccaagttc tatagcccgg tgtagggcgg ctaatgattc agcgtctttg
     6361 accggccccc aggttccgcc aatcgcccat gcgccaaaac tcaaggcaga gactttccaa
     6421 cctgtgcgtc ccagtactcg atattgcatg aacatgcctc cagatccaca ttcgtcacct
     6481 gaaataaaaa tacaatgaat gtcacactat tcactctcca cttcaatata caccttgatc
     6541 gctttcttct cttcggtcaa caagcggatc atctcttgat attgatctag ccctttcaat
     6601 ggatgtgtga gaaatctttt caaccagccg ggccaatact gttcagccaa tgccatatct
     6661 tgcacgccca tctcgaagta cactcgattg gcattgaccg acccgaccat cactttgttg
     6721 ccgagcacaa atttc
//
LOCUS       DASRTJ010000127         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7536,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000127 DASRTJ010000000
VERSION     DASRTJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7536"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFF70_02615"
     CDS             complement(<1..227)
                     /locus_tag="VFF70_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY43617.1"
                     /translation="METEFLKHLPLFSGLADHDLKWLIDQARPIAISAGEVLIEEGAL
                     GDSMFIILDGEFEVSKRSAQQDIKLDVREQGA"
     gene            complement(296..1951)
                     /locus_tag="VFF70_02620"
     CDS             complement(296..1951)
                     /locus_tag="VFF70_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY43618.1"
                     /translation="MAKPVIFTIDDDRNVLSAIERDLRKKYGRDYRILKAESGAAALE
                     SIQQLHQRSEPIALLIADQRMPQMTGVQLLEQSMDLFPDAKRVLLTAYADTEAAINAI
                     NLVGLDYYLLKPWDPPEDKLYPMLDDLLDEWKAHVRLPFDGIRVAGTLWSNEAHEIKD
                     FLVRNQIPYQWLDIEADPKAHALVEQANNGVLKIPTVFFADGSILIQPDIRAVAEKAG
                     ISTHAHLKFYDVIVIGGGPAGLAAAVYASSEGLSCLLIERHAPGGQAGSSPKIENYLG
                     FPVGISGGELTRRAMTQAVRFGTEILSAQEVVKVRTQDHYHLVTCNDGTEVSCHALLF
                     ATGASFHTLTMPGAAELTGKGIYYGAAHTEASFYKDQDVFIVGGANSAGQGAMYLTRF
                     ARTVTVLIRGPELTASQYLVDALRQNPKIEICLNTDLLEVRGSDKLEEIVIKNTETQA
                     VKTLPAAALFVFIGVRPQSDLAADLLERDAKGYIYTGPDLLREGKRPISWKPDRDPMM
                     METSVTGIFAAGDVRHGTNHRVASASGEGGIAVASIKQYLKTL"
BASE COUNT          443 a          572 c          585 g          488 t
ORIGIN      
        1 gcgccttgct cgcgcacgtc gagcttgatg tcctgctgag cagatcgctt gctgacttcg
       61 aactcgccat cgagaatgat aaacatggaa tcgccgagcg cgccttcttc gatcaagacc
      121 tcacccgcgc taatcgcgat cggccgggct tgatcgatca gccatttgag atcgtgatcg
      181 gcgagtccgc tgaataaggg caggtgtttt aagaattcgg tttccatgag atccgtttgt
      241 ctgtcagggt gagcggccgc tcgcctgagg cagtccgccg actgcgctga caatatcaca
      301 acgtcttcaa gtattgcttg atcgatgcca cggcaatgcc gccttcgccg ctggctgaag
      361 cgacgcgatg attggtgccg tgtcggacat cgcccgcggc gaaaatgccc gttacgctgg
      421 tctccatcat cattggatcc cgatcgggtt tccagctaat cgggcgtttg ccttcgcgca
      481 agagatcagg gccggtgtaa atgtagcctt tcgcatcacg ctcgagcaga tcggccgcca
      541 gatcgctctg cggacgcacg ccgatgaaca cgaatagcgc cgcggcaggt aacgttttca
      601 ctgcttgcgt ttcagtattc ttgatcacga tctcttcaag tttatcgctg ccgcgcacct
      661 caagcaaatc ggtgttgagg caaatttcga tctttgggtt ttgtcgcagc gcatcgacga
      721 gatattgcga cgcagtaagt tccggcccgc gaatcagcac ggtgactgtg cgcgcgaagc
      781 gagtgaggta catggcgcct tgtcccgccg aatttgcgcc gccgacgatg aacacatctt
      841 gatctttata gaagctcgcc tcggtatgcg ccgcgccgta gtaaatacct ttgccggtta
      901 gttccgcggc gcctggcatc gtcagggtgt ggaaagatgc gccagtggcg aacagcaacg
      961 cgtgacatga aacttccgtg ccatcattgc aggtgaccaa atgataatga tcttgcgtgc
     1021 ggactttgac gacttcttgc gcagataaaa tctcagtgcc gaagcgaacg gcttgcgtca
     1081 tcgctcggcg cgtgagttca ccgccggaaa tgccgacggg gaagccgaga taattttcga
     1141 tcttggggct gctgcccgct tggccgccgg gcgcatgtcg ttcgatcaat aagcagctca
     1201 agccttctga gctcgcatag accgcagctg ctaaaccggc tggcccgccg ccgattacga
     1261 ttacatcata gaacttcaag tgcgcgtgtg ttgagatgcc ggctttctct gcaacggccc
     1321 gaatatccgg ctggatcaag atcgatccat ctgcgaagaa aaccgttgga attttgagca
     1381 cgccgttatt ggcttgctcg acgagcgcgt gggcttttgg atccgcctca atatccagcc
     1441 actgatatgg aatttggttg cgcactaaaa aatctttgat ctcgtgcgct tcattcgacc
     1501 acaacgtgcc cgcgacgcga atgccatcga acggcaaccg cacatgcgcc ttccactcgt
     1561 cgagcaggtc gtcgagcatg ggatatagct tatcttcggg gggatcccac ggtttgagca
     1621 aatagtaatc gaggccgacg agattgatcg cattgatcgc cgcttcggta tcggcatacg
     1681 ccgtgagcag cacgcgtttt gcgtcgggaa acaagtccat tgattgttcg agcagctgga
     1741 cgccggtcat ctgcggcatg cgttgatcgg cgatgagcaa tgcgatcggt tcggatcgct
     1801 gatgcaattg ctgaatcgat tccaaagcgg ccgcacccga ttcggctttg agaatgcggt
     1861 aatcccgacc gtacttcttg cgcagatcac gttcgattgc gctcaggacg ttgcggtcgt
     1921 cgtcgatggt gaagataacg ggttttgcca aaattgattt ctccgatatt ctttgaatcg
     1981 atagaagtca ttcccgcgta cgcgggaatc cagaatggtt agaactggac acccgcttgc
     2041 gcgggtgtga caggcgaatg atcaaatcac caactcacca cgcaaaac
//
LOCUS       DASRTJ010000128         7530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_45443,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000128 DASRTJ010000000
VERSION     DASRTJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45443"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFF70_02625"
     CDS             <1..210
                     /locus_tag="VFF70_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZY43619.1"
                     /translation="VQRALVEHGGIQCGFCTPGMIMASIALLKEYPRPTEDEVRFSLA
                     GNLCRCTGYKKIVEAVLSVAEGVKP"
     gene            207..2534
                     /locus_tag="VFF70_02630"
     CDS             207..2534
                     /locus_tag="VFF70_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZY43620.1"
                     /translation="MNTAIGQRQPKLDAPDKATGRTVYGHDVRLPGMLHGQSLYSRYP
                     HARILNVDVSRALKLPGVKTIITAADNPPTKFGYGKDNTPLKGDVVRSLRDEVAAVAA
                     IDPDIAAEALELIEVEYEPLEPLFDAAAALKDDAPLIHPEHGTNRFSHFNYTHGDLEQ
                     GERDSDVIVEADYELPYVVHAAMETSIAIASFDHRGHLTLYSTTQIPFLLQRDLSEAL
                     GLEGSDVRIIQTAIGGAFGRGLDIYPFEPIAALLARKAGQPVRLSFSRHEEFLAAPVR
                     QPVKAHIRAGATRDGQVTFRDVYALLDIGAYVSWGSVTPLVMMETTASLYRVPHVRFT
                     ADCVYTNNPITGAVRGYGNPQSTFWVETTMDRLAEALQLDPIDFRIRNANRPNEETPQ
                     GLKITSCGLKECLEAVAARADVEVWNVEFGMRNGRNRSSTPHSALPTPHLKRGIGFAS
                     TLNVGGGARIYRSDGCGATVKIDDFGHVSLITGSTEIGQGSETVLAQIVAEVLGVSID
                     DVNVINSDTDVKPWDVGVHASRTTFIAGNAAHIAAIDARRQVFETAAPMLGVAPDQLA
                     MRGGAVVTLSGDKSIELGKVARARHFRAGGQIISGEGFYDPPTQLVDKDSYKGNISAA
                     YGFGAQMADVEVDTETGHVRVLRLVCANDVGRAINPMAVEGQIEGGAQMGLGYALTEE
                     LIVKEGRVLNPDFHDYRLFTAADMPELESIIVETDDPLGPFGAKGVGEMGGTPTAAAI
                     ANAIYDAIGVRMTTVPMTPERILKALFEKEGRDIC"
     gene            2528..3658
                     /gene="hisC"
                     /locus_tag="VFF70_02635"
     CDS             2528..3658
                     /gene="hisC"
                     /locus_tag="VFF70_02635"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZY43621.1"
                     /translation="MLTSLRLNPNLLKVPLYIAGKSIEEVQEEYGLDDVLKLASNESA
                     LGPSPLAIEAAQRVLWDSHRYPGSADRNLRRRLAPTLHPNFDEHYLIIGNGATDIIRM
                     LAQAFVFDGGEMITSRVTFPMYHIAATMFGGVPVLVPPTSDLRFDLRAMAEAIKPNTR
                     LIFVCSPNNPTGMICQRAEVEQFMRRVPAEVVVVFDESYRDFVADPDYPDPCDYVDDG
                     RNVIVVRSFSKSAGLANLRVGYAIARPEMIEYLSHAQIPFNTGALALEAARASLDDHE
                     YLERIRRLVCEEREFLYHALDGLDLAYTRSHANFVMIPDLPLEANRLSEKLIRSGVIV
                     RPMAAWGMPTAIRVTIGLREHNERFIAALGAALADGNGTSKQ"
     gene            3696..3890
                     /locus_tag="VFF70_02640"
     CDS             3696..3890
                     /locus_tag="VFF70_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43622.1"
                     /translation="MIETEPVKQCVNCGRSVEQVPLISIAHRDGAAYICPQCLPILLH
                     QPQALVGKLPGAESLRGHAH"
     gene            3913..4350
                     /locus_tag="VFF70_02645"
     CDS             3913..4350
                     /locus_tag="VFF70_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY43623.1"
                     /translation="MDYIKRVERVALAVEDLDKAQAFFEQWFGAKFQPEEFIEDMAIR
                     YRPFDIGASKMELLQATRDDSPVAKFIQKNGGPGVHHITFEVDDLDAAIAELEQRGGH
                     IAYRHTYQPGVTFEGQVWREAFVHPKDAFGVLIHLAEKKPIGN"
     gene            4479..5618
                     /locus_tag="VFF70_02650"
     CDS             4479..5618
                     /locus_tag="VFF70_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY43624.1"
                     /translation="MDFSFTEEQELFRKSVRDFVEKEVVPHAREIDERGEFPMELFRR
                     CGQLGFFGLRYPESVGGLGADFTTFCLMVEEIARGLLSLAAAVSMQCLMGTDFVYRFG
                     TDDHRTRLLTPALRGEMLGTIAMTEPDFGSDLGGITTRAIKDGADWILTGRKMWITSA
                     TVADFFTVAAKTDPEAGFKGIDMFLVEKNMPGLAVGKRIEKMGVRASETSEVILENVR
                     VPAENLLGQQGTGFKNLGAVLAEIRTMTGALAIGLAEAALEASIKYAHERIQFGKPIG
                     AYQSIAHKIAEMGTQLEAARGLVQRAAWLIDMKQPDMKLASMAKLFATEMANKAADEC
                     TRIFGSYGLAMEYDAQRYFRDARFLLYGGGTSEILRGIIAKELGV"
     gene            5649..6098
                     /locus_tag="VFF70_02655"
     CDS             5649..6098
                     /locus_tag="VFF70_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZY43625.1"
                     /translation="MKTRGLTFEQFNLDDVFTSQSRTVTEADVVNFAGLSGDFNPLHT
                     DEEFGKTMPAGTRIAHGMLIAAMATGMSNWTGVFEGTTIALMEQLIQYKGLVKFGDTI
                     HLELKVAEKKETSKPDRGIVFFETRVVNQEGKAVIEGKWTLMMKRSN"
     gene            6155..6922
                     /gene="fabG"
                     /locus_tag="VFF70_02660"
     CDS             6155..6922
                     /gene="fabG"
                     /locus_tag="VFF70_02660"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZY43626.1"
                     /translation="MRLKDKVALITGAGGGIGQATAIRFAQEGARIIAVDLDEAGIAE
                     TLKLISESGGTAIAAVGNVTKRSEVQQMINTAIQNFGRLDIAINNAGITRDGLTVRIK
                     DGEVKAMTDEQWDAVLEVNLKGTWLVSQLAAVPMMKQKYGRIVNTASVGALGNIGQAN
                     YAASKAGVIGLTKTLALEWARFNIAVNCVAPGGTKTRMTAAIPEAVAAALIDRIPLKR
                     FAEPAEIANVHLFLASDEASFITGEVIFADGGQTVGA"
     gene            6948..>7530
                     /locus_tag="VFF70_02665"
     CDS             6948..>7530
                     /locus_tag="VFF70_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZY43627.1"
                     /translation="MFENVFIPYSGYWCTPFVKWQGNFASLHPLTFAADMTKRALADR
                     QIDPKSFEALYLGMTVPAKSSFYGAPWLAGLIGADQITGPTINQACATSARVIGDAAH
                     DVETGNGSDRSILCITADRTSNGPHVIYPNPIGPGGKPEAEDWVWDNFNHDPFARNAM
                     LETAENVAREAEITTVEQHEVMLMRHGQYQAALK"
BASE COUNT         1680 a         2041 c         2312 g         1497 t
ORIGIN      
        1 gtgcagcgcg cgctggtgga acacggcggc attcaatgcg gattctgcac acccggtatg
       61 atcatggctt cgatcgcgct gctgaaagaa tatccgcggc ccactgaaga cgaagtgcgg
      121 tttagcctgg ctggcaattt gtgccgctgc acgggctata agaaaatcgt tgaagccgtg
      181 ctgtcggtgg cggagggtgt gaagccatga acaccgccat cggtcaacgc cagcctaaac
      241 ttgatgcgcc ggacaaagcg accggccgca cggtgtatgg tcatgatgtg cgtctgcccg
      301 gcatgctgca cggtcaaagt ctatattcgc gctatccgca cgcgcgcatt cttaacgtcg
      361 atgtgtcgcg cgcgctgaaa ctgcccggcg tcaaaacgat catcaccgcc gccgataatc
      421 cgccgacaaa gtttggttac ggcaaagaca atacgccatt gaagggcgac gtcgttcgca
      481 gtctgcgcga tgaagtggcc gccgtcgccg cgatcgatcc cgatatcgcc gccgaagcgc
      541 tggagttgat cgaggtggag tatgagccgc ttgagccgct attcgatgcg gccgccgcgt
      601 taaaagacga cgcgccgctc attcatcccg aacacggcac caatcgattc agccacttca
      661 attacacgca cggcgatttg gaacaaggcg agcgcgacag cgatgtgatt gtcgaagccg
      721 attacgaatt gccgtacgtg gtgcacgccg cgatggaaac cagcatcgcc atcgcctcgt
      781 tcgatcatcg cggccacctc acgctgtata gcaccacgca aattccattt ctactgcagc
      841 gcgatttatc tgaagcgctc ggtctggaag gcagtgacgt ccgaatcatt caaacggcga
      901 tcggcggcgc gttcgggcgc gggctggata tttatccgtt cgaaccgatc gccgcgctgc
      961 tggcgcgcaa agcaggccag cctgtccgat tgtccttctc gcgtcacgaa gaatttctgg
     1021 ccgcgcccgt gcgccaaccg gtgaaggctc atattcgcgc gggcgcgacc cgcgacggac
     1081 aagtaacctt tcgcgatgtg tacgcgctgt tggacatcgg cgcgtatgtg tcgtggggca
     1141 gcgtcacgcc gttagtgatg atggagacca cggcttcatt gtatcgcgtg ccacatgtgc
     1201 gcttcaccgc cgattgtgtc tacaccaaca atccgatcac gggtgcggtg cgcggctatg
     1261 gcaatccgca atcgaccttc tgggtggaaa cgacgatgga tcgattagcg gaagcgctgc
     1321 agctggaccc gatcgatttt cgcatccgca atgccaatcg gccgaacgaa gagacgccgc
     1381 aaggcttgaa gatcacatcg tgcggtttga aagaatgtct ggaagcagtt gctgcaagag
     1441 cagacgtaga agtgtggaat gtggaattcg gaatgcggaa tggacgtaat cgatcgagca
     1501 ctccgcactc cgcactcccc actccgcatt tgaaacgcgg catcggcttt gcctccacgc
     1561 tcaacgtcgg cggcggcgcg cgcatctatc gcagcgacgg ctgcggcgcg acggtgaaga
     1621 tcgacgactt cggccatgtg tcactcatta cgggcagcac cgagatcggg caaggctcgg
     1681 aaacggtgct ggcgcagatt gtggccgaag tgctcggcgt cagcatcgac gatgtcaatg
     1741 tgatcaacag cgatacggat gtgaagccat gggatgtggg cgtgcatgct agccgcacga
     1801 cgttcattgc gggcaacgcc gctcacatcg cggcgatcga cgcgcggcgg caggtatttg
     1861 agactgcggc gccaatgctg ggggttgcgc cagatcagtt ggccatgcgc ggcggagccg
     1921 ttgtcacatt gagcggtgat aaatcgatcg agttgggcaa agtcgcgcgc gcgcggcact
     1981 ttcgcgccgg cggacaaatc atcagcggcg aaggctttta cgatccgccc actcaactcg
     2041 tcgacaaaga cagttacaaa ggcaacatct cggcggcgta cggcttcggc gcgcagatgg
     2101 ccgacgtgga agtggatacc gaaacagggc atgtgcgcgt gctgcggctg gtgtgcgcga
     2161 atgatgtggg ccgcgcgatc aatcccatgg ccgtcgaagg acaaatcgaa ggcggcgcgc
     2221 aaatgggtct agggtatgcg ttgactgaag aattgatcgt gaaagaaggc cgcgtcctca
     2281 atcccgattt tcatgattat cgattattta ccgcggcgga tatgccggaa ctcgaatcga
     2341 tcattgtcga gaccgatgat ccgctaggac cattcggcgc gaaaggcgtc ggcgaaatgg
     2401 gcggcacgcc gacggcggcc gcgatcgcta acgcgattta cgatgcgatc ggcgtgcgca
     2461 tgacgaccgt gccgatgacg ccggagcgca tactgaaagc actgtttgag aaagagggcc
     2521 gtgacatatg ctgacatcac tgcgcttaaa tccgaatctg ctgaaggtgc cactctatat
     2581 cgcgggcaag tcgatcgaag aagtccagga agagtatgga ctggacgatg tcctcaagct
     2641 cgcctcgaac gaaagcgcgc tcggcccgtc accgctggcg atcgaagccg cgcagcgcgt
     2701 gttgtgggat tcgcatcgct atccgggcag cgccgatcgc aacctgcgcc ggcggctcgc
     2761 gcccacgttg catccgaact tcgacgagca ttacctcatc atcggcaacg gcgcaaccga
     2821 cattatccgg atgctcgcgc aggcgtttgt cttcgacggc ggcgagatga tcaccagccg
     2881 cgtgacgttc cccatgtacc atatcgcagc gacgatgttt ggcggcgtgc cggtgctcgt
     2941 gccgcccacg tctgatttgc ggttcgatct gagggcgatg gcggaagcga tcaagcccaa
     3001 tacccgattg atcttcgtct gctcaccgaa taacccgacc ggtatgatct gccagcgcgc
     3061 cgaagtggaa cagttcatgc gtcgcgtgcc cgctgaagtc gtggttgtgt ttgatgaatc
     3121 gtatcgtgat tttgtcgcag accccgatta tcccgatccg tgcgactacg tcgatgatgg
     3181 ccgcaatgtg attgtagtcc gcagtttcag caagagcgcg ggcctggcga atttgcgggt
     3241 gggttatgcc atcgcgcggc cggagatgat cgagtatctc agtcacgcgc agattccatt
     3301 caataccgga gcgttggcgt tggaagccgc gcgcgccagt ctcgacgatc atgaatatct
     3361 tgagcgtatc cgacggctgg tgtgtgaaga gcgcgagttt ctctaccacg cgttggatgg
     3421 gcttgatctg gcgtatacgc gcagccacgc gaactttgtg atgattccag atctgccgct
     3481 ggaagccaac cggctgagcg aaaaattaat ccggtcgggc gtgatcgtcc ggccgatggc
     3541 ggcctggggt atgcccaccg cgatccgcgt cacgatcgga ctgcgcgaac acaacgagcg
     3601 gttcatcgcg gcactgggcg cggcgttggc agacggcaac ggaacaagca aacaatgatc
     3661 ggtcgctcta ctcaagcgac gcaaggcgaa acaacatgat tgaaactgaa cccgtgaaac
     3721 aatgtgtgaa ctgcggtcga tcggtcgagc aggtgccgtt gatctcaatc gcccatcgcg
     3781 acggcgcggc ctatatctgc ccgcaatgtc tgccgatctt gcttcaccaa ccgcaagcgc
     3841 tggtgggcaa actgcctggc gctgagtcgc tgcgcggtca cgcgcactaa ttcaacgaca
     3901 aaaggcaatc gcatggacta catcaaacgc gttgagcgcg tcgccttagc ggtcgaagat
     3961 ttggataaag cgcaggcttt cttcgagcag tggttcggcg ccaagttcca gcctgaagaa
     4021 tttatcgaag acatggcgat tcgctatcgt ccgttcgaca tcggcgcgag taaaatggaa
     4081 ttgctgcaag ccacgcgcga tgacagcccg gtggcgaagt tcatccagaa gaacggcgga
     4141 ccgggtgtgc atcacatcac gtttgaggtg gacgatctcg atgcggcgat cgcggaattg
     4201 gaacaacgcg gcgggcacat tgcatatcga catacgtatc aaccgggcgt gacgttcgaa
     4261 gggcaagtgt ggcgtgaagc gtttgttcat ccgaaggatg cgttcggcgt gttgattcat
     4321 ctcgccgaga aaaaaccgat tggcaattag caattaacac tgagcaatga acaaagcgtc
     4381 attgcgagga gcagcttttt gcgacgaagc aatctctcgc tcaacaagaa tgaggttgct
     4441 tcgcaaaagg tgctcgcaat gacgaacgac gggtgaagat ggacttttca ttcactgaag
     4501 aacaagaact atttcgcaaa tccgtgcgcg acttcgtaga gaaggaagtt gttccgcacg
     4561 cccgcgagat cgacgaacgc ggtgagttcc cgatggaatt gtttcggcgc tgtggccaac
     4621 ttggattctt tggcttgcgt tatcccgaat cagttggcgg actcggcgcg gactttacga
     4681 cattttgttt gatggttgaa gaaatcgcgc gcggattact gtcactggcc gccgccgtat
     4741 cgatgcaatg tttgatgggc accgacttcg tctatcgctt tggcacggac gatcacagaa
     4801 cgcgcttgct caccccggcc ttgcgcggcg agatgctcgg cacgatcgcc atgaccgagc
     4861 cggacttcgg atcggatttg ggcggcatca ccacccgcgc gatcaaagac ggtgctgatt
     4921 ggattctcac tggccgcaag atgtggatca cgtccgcgac ggtggccgac tttttcacag
     4981 tggcggctaa aaccgatccc gaggcgggct ttaaaggcat cgatatgttt ctggtcgaga
     5041 agaacatgcc gggcttagcg gtcggcaagc ggatcgagaa gatgggcgtg cgcgcatcgg
     5101 aaacatcgga agtgatcttg gagaatgtgc gcgtgcccgc tgagaacttg ctcggtcaac
     5161 aaggcacggg ctttaaaaat ctgggcgcgg tcttagcgga gatcaggacg atgacgggcg
     5221 cactcgcgat cgggttagcc gaagccgcgc tggaggcgtc gatcaaatat gcgcacgagc
     5281 gcattcagtt tggtaaaccg atcggcgcat accaatctat cgcgcacaag atcgccgaga
     5341 tgggcacaca gttggaagca gcgcgcgggc tggtgcagcg cgcggcgtgg ttgatcgata
     5401 tgaagcagcc cgatatgaaa ctcgcctcga tggccaagtt gtttgcgacc gagatggcga
     5461 acaaagctgc cgacgaatgc acgcgcatct tcggcagtta cggtttggcg atggagtacg
     5521 acgcgcaacg ttactttcgc gacgcgcgct ttctgttgta tggcggcggc acgagcgaga
     5581 ttttgcgcgg catcattgcg aaagaactgg gcgtgtgaat ttcagcgggg taaatcacag
     5641 gagagtaaat gaaaactcgt ggcttgacgt ttgaacaatt caatctcgac gacgtattca
     5701 catcgcaatc tcgcacggtg actgaagcgg acgtagtgaa cttcgccggg ctgtccggcg
     5761 atttcaatcc gctgcatacc gatgaagaat ttggcaagac catgccagcg ggcacgcgca
     5821 ttgcgcacgg catgttaatc gccgcgatgg cgaccggcat gtccaactgg acgggtgtgt
     5881 ttgaaggcac gaccatcgcg ttgatggagc agttgattca atacaagggc ttagtgaagt
     5941 tcggcgacac gattcatctt gaactgaaag tagccgagaa gaaagagacc tccaagcccg
     6001 atcgcggcat tgtgttcttt gagacgcgcg tggtcaatca agagggcaag gccgtcatcg
     6061 aaggcaagtg gacgttgatg atgaaacgca gcaattagca aataacaatg agcaatgttc
     6121 aattatcaat gctcaatgaa aaggacaaac gatcatgaga ctaaaggata aagtggcttt
     6181 aataacgggc gcgggcggcg gcattgggca ggcgacggcg attcgctttg cccaggaagg
     6241 cgcgcgcata atcgcggtcg atctggatga agcgggcatc gctgaaacgc ttaagttgat
     6301 tagcgagagc ggcggcacgg cgatcgcggc cgttggcaat gtgaccaagc gatcggaagt
     6361 gcagcagatg atcaacaccg cgatccagaa cttcggccga ttggatatcg ccatcaacaa
     6421 cgccggtatt acgcgcgacg gtttgacggt gcgcatcaaa gatggcgaag tcaaagcgat
     6481 gacggacgag caatgggacg cggtactcga agtcaatctc aaaggcacgt ggctggtctc
     6541 gcaactggcc gcggtgccga tgatgaaaca gaagtatggc cgcatcgtca acacggcttc
     6601 ggtgggcgcg ctgggcaata tcgggcaggc gaactatgcc gcatccaaag ccggcgtgat
     6661 cggcttgacc aaaaccttgg cgctggagtg ggcgcgcttc aacatcgccg tcaactgcgt
     6721 cgcacccggg ggcacgaaaa cgcgtatgac ggccgccatc ccggaagcgg tggctgccgc
     6781 gttgatcgat cgcatcccgc tcaagcgctt tgcggaaccg gcagagattg ccaatgttca
     6841 tctatttctc gccagcgacg aggcctcctt catcaccggt gaagtgatct ttgctgatgg
     6901 cggacaaacc gtcggcgcat gaggccgact gacccacagg agtgatcatg tttgaaaatg
     6961 ttttcatccc ttacagcgga tattggtgca cgccttttgt gaagtggcag ggcaactttg
     7021 ccagtctgca tccgttgacc ttcgctgcgg atatgaccaa gcgcgcgttg gccgatcggc
     7081 agatcgatcc gaaatcgttc gaggcgttgt acctgggcat gaccgtgccg gcgaagagtt
     7141 cgttttacgg cgcgccctgg ctggccggat tgatcggcgc tgaccagatc accggcccga
     7201 cgatcaatca agcctgcgcc acctccgcgc gggttatcgg cgatgccgcg cacgatgtcg
     7261 agaccggcaa tgggtcagat cgatcgatct tgtgcatcac cgccgatcga acctcgaacg
     7321 gaccgcatgt gatctacccc aacccgatcg ggccaggcgg caaaccggag gccgaagatt
     7381 gggtctggga taacttcaac cacgatccgt ttgcccgcaa tgcgatgctc gagacagccg
     7441 agaacgtggc gcgtgaagca gagatcacca ctgtcgaaca gcacgaagtc atgctgatgc
     7501 gtcacgggca gtatcaagcc gcgctcaaag
//
LOCUS       DASRTJ010000129        10532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_82941,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000129 DASRTJ010000000
VERSION     DASRTJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82941"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFF70_02670"
     CDS             <1..697
                     /locus_tag="VFF70_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43628.1"
                     /translation="LCAALIAWVFLTPSIDQPAQPLPAGANLMDVFAPLLVASAAIER
                     FLETIFNVIENSWHTLIAYFGRGLRWLKSGEAEVERSRQWLADVSNFYNTELNHLGID
                     PATAGGPATVNAMLRLNSLEAMLTAARQRLDTAEKQLNDLTESSSYKSAKATAAIILG
                     LMIGVVTAKFADLQMFALLGINAVPTKIDIFITGLIIGSGTYPVHSLVGLLQQFKDVL
                     DSTRQAPKPIRSK"
     gene            724..1800
                     /locus_tag="VFF70_02675"
     CDS             724..1800
                     /locus_tag="VFF70_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZY43629.1"
                     /translation="MAEISRKELILALVSAGDHPRLSGLDLSTLDLSLLDLHGARIIL
                     ANLMRADLRGVNLSEANLTESNLMQSNLSGANLSKAILSCVDLTQALIRRAILNGALL
                     RSANLKNAILIEASLVGADLSQANLHRADLSHANLSEANLTKADLSEASLEGATLSQA
                     ILTGVSLHRANLVGINMSGANLSGIDASQAALSKSDLSQANLTRANLGGADLSLADLI
                     EANAHFVNLRSAHLVRTNLHGALLHGATIQQADLSGADLSGTDLSNADLTDANLSGAN
                     LTKANLKGARLYGAQLFKANLSNALLEEAILTKADLTGANLKGAVLTDANLDQINLDK
                     ALLRDAVLRESKVNARQFYKASMS"
     gene            1888..4380
                     /locus_tag="VFF70_02680"
     CDS             1888..4380
                     /locus_tag="VFF70_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HZY43630.1"
                     /translation="MGGFLLHVPYKSQNDADATLKRSDCGPACVAMILNASGQNVTTN
                     QVTKASGVIGDQGVRINQLKIAAKVFALELNSRSGLTLDDLKAALNNGQPPIALINYG
                     LIPDRWDRNYSGGHFVVLVGYDDAAGRVFINDPDYPPGAQGYQRPYAFDVFSRAWSGF
                     ARNQNPNNCLIVPQLTRSISGTPIGTPPPSPNGSAVHAWVTMPHGLPFWSQPNTHSQL
                     INDLVYAQHVSQIDSTSGWLQVITDSGLSGWVADRFNNSPTLSNTQPPAGYPVEVLDT
                     LPIRAVGGWSVRAQRNLNAPRADRIAAGEHATVYMYFMDTDNTPWLLVQSPRGQSGWG
                     RESTGSIALVRTIDLHTKDDTSTTTPGTPPAPNPAPIHSSDVWVTAIDGLVLRENPDP
                     SAHKIASLPFGQRLIAIGPESPRDNTGRAWQPVRIENGSTGFVVAGFGAERYLSLTRP
                     PKPYTLEVLDTQFARSQAGLSILQSHEDSAPVIDRAQIGEHLTAYNRFTDNTGSWLWL
                     ESPRNKAGWLRDKVDGAILVKPIDPGPTPHPIVDPRPFGKCLTGLGMANPQPLTQLEF
                     DLIGKSKIEAFKILTLPDPNDTVQLIRTLKNIPTIKFIVARLFFSVDAENKTRFSPQT
                     FVETVFQSASAAYQAGLRYFEIHNEPNLENEGLNWNWRNGAEFGAWLSQVMSFLRARL
                     PEAQLGFPGLSPQPNAPDFLNGAQSALRQCDWLGAHSYWQSIEGGPYPMAASNAGMYW
                     RSIRDQFPNKLVMITEFSNNNSGVDLEEKGRQYTRYYQLLRNEMNLGAAFSFALNWPG
                     QDANHEGWEYDGRETTIPGIVGSVLGQAGFLG"
     gene            complement(4475..4972)
                     /locus_tag="VFF70_02685"
     CDS             complement(4475..4972)
                     /locus_tag="VFF70_02685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY43631.1"
                     /translation="MLIQYWPTQYTVITNRFGTDPAYYKQYGLPGHEGLDVRVQTRAA
                     VSPIYCCTEGQITFIGYRTPKDPYGYQVRVHLEYQGREYEFIYAHMVDQSCDLIVGSP
                     VHPGTIIGTGGATGNARGAHLHISICKVGATRNKETTFPSDLINPEPLFKEYIQFAGL
                     EVEWL"
     gene            5135..5443
                     /locus_tag="VFF70_02690"
     CDS             5135..5443
                     /locus_tag="VFF70_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43632.1"
                     /translation="MESSTLINIGFGLVLGFLIGWLVEWRLDVSFWNSHIAQLNRREA
                     ARRTQADKEIEEAQIEAGQQVADLRRQLEAEIAELRARSEERVAAARAKTETEPSRIR
                     "
     gene            5660..5830
                     /locus_tag="VFF70_02695"
     CDS             5660..5830
                     /locus_tag="VFF70_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43633.1"
                     /translation="MELKRKAISLELSRSYLIALEELYGQRLAGKLQIALIASFTLIL
                     MVGLMVATHSFG"
     gene            5854..6546
                     /locus_tag="VFF70_02700"
     CDS             5854..6546
                     /locus_tag="VFF70_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43634.1"
                     /translation="MADTDPNSSARNVWSTVNGSYRGHIRRKRIVAAVIILLLLAPLT
                     YLVVNQIIQARAQSPDPVSVSAVEVTTTSTLSQGSGVIDTPTLAQFVSVTPRVLPSET
                     ATAVRVVDTSTAAAPTTPQPTRTSIQTQIPRLTTTATTSAQNVAASTSTSTPRPTNTL
                     QPTATSIAAAQSVSPTPTATPTAQPTLQIVIPATGGETPGLNLFQILGLILLGMVLIL
                     LGSALIAPPARR"
     gene            complement(6568..7749)
                     /locus_tag="VFF70_02705"
     CDS             complement(6568..7749)
                     /locus_tag="VFF70_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43635.1"
                     /translation="MSIDQILLFVGLGIAAYFLMRQAVRRAVRSGVPTQVYLRYVYPR
                     RVAVAYDRGDAPLAERQIYQAYLAQLTAPKDADRELAIQYLEQLPISQELVDHLIEAL
                     PQQSKRALQLRMVQLLRTAMIELEHSSYAAKDNFESGLPEWSRAISVWLAVIILVALR
                     WSWLTQLAGAYFIVITVVLLGLALLAVTWVTTDRKMFVRVAGLMSGLVLAGLWFFSNN
                     SHAGLDTTPMKSLENYGLGATQIQITYPRWLTIDNVGACTAGVTITVFGTPNPPVNPI
                     EISFLYNHADLFVGDNNCHALSPSFNLNTANTTGLPYQFYIMLPRRDVLAKRQIVITP
                     QVRDSAQNGQPYPATNLEFTIQLEDPLWEGIRNVGQILFGATTVSSFLVALYAWYQRR
                     S"
     gene            complement(7761..8216)
                     /locus_tag="VFF70_02710"
     CDS             complement(7761..8216)
                     /locus_tag="VFF70_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43636.1"
                     /translation="MNKRILWIEDDYYAIKGLVRPLEKAGFEVDAATSAIDGFNKALQ
                     WEQYDLIVVDLILPISNDNKPVPEKVSAWQKEEHVGVGILKWLLVELKVTRPILLLTV
                     VRNSAQAYKLDSLGLAGYLPKRGLLPSQVKTEVFRLLGINSDAPDAIRL"
     gene            complement(8287..>10532)
                     /locus_tag="VFF70_02715"
     CDS             complement(8287..>10532)
                     /locus_tag="VFF70_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43637.1"
                     /translation="AGDLKWLDRYGEARSFYRASEAKPIMIVPLIAGRGTLGVIKFMA
                     KIDQQPFDRTDEDLAVIAARFLANAIDQAKIIQQQSVHIRQLVEMGAKQDAQQLFEAV
                     TDRLSSMLGAHSQLYLRAKGGSEVRLIVENGRRVDQETSIARGNDLIGWVFKTGKRLS
                     LPDAQAFMHGERLADVDLDRISDGAVINDDDRFLKSDLIEVADLSTAPLPFLGVPVKT
                     HDGSILGVLCAQALSAASQLRQRSFDRDEVQLAQAFASAIALAINNDRARRLGDLLMQ
                     VGFQADLDKLFDLIVQQVPLLVAGTGCSIYLAENVDAPCLRLARSSRAGLSDADRAKA
                     ITYQYGEGKTGFCALARATLVVNHFGVGAASQIALKAERTRLQARQSGDLIENLLDDQ
                     KQLVGLIQLRRGLSSAAHVQQKFTELAQQLLADRVKGLPTAKKAEFDRAGSKASWSFI
                     AVPIQIDAAELFGVITIGRPIDQSPFFAEDVALVESIAGRLAAIMRNLKTQDDQKRLL
                     MSLAHEINTPLQGVMADTENLKEELPAGSELKQLAGHTLEQVQKLHLLTETIMTVLSG
                     QAPERALTIHSLYRPLKEACQMFESEAAAKGCDILDPRAIGSRFPDIEMSLFDLTIAF
                     KNLIHNAIKYSFKPPQKYAGQRYVRITGQWADPDRQAYAINIENYGVGIPPEEIDSRL
                     IFRPYYRGAKASDRRRTGAGLGLAQVRQIIEEMHHGSIGVTSKPVVGEAHLTTFTVTL
                     PVRQPKQ"
BASE COUNT         2482 a         3045 c         2719 g         2286 t
ORIGIN      
        1 gctatgcgct gcgctgatcg cgtgggtatt tttgacaccg tcgatcgatc agccggcaca
       61 gccgctgcct gcgggcgcga atttgatgga cgtattcgcg ccgctcttgg tcgcttcggc
      121 agcaattgaa cgttttttgg aaaccatctt caatgtcatt gaaaacagct ggcatacctt
      181 gatcgcctac tttggccgcg gactgcgttg gttaaaaagt ggcgaggcgg aagtcgagcg
      241 atcgcgccag tggctggccg acgtttccaa tttctacaat accgaactca atcatctggg
      301 catcgatccg gcaaccgcgg gcggcccggc cacggtgaat gcgatgctgc gtcttaactc
      361 gctggaagca atgctcacag cggcacgcca gcgcctggat actgccgaga aacagctgaa
      421 tgacctgacc gaatcgagca gctacaagag cgcaaaggct accgcggcta ttattttagg
      481 tctgatgatc ggcgtcgtca ccgctaaatt cgccgacttg caaatgtttg ctctgttggg
      541 catcaatgcg gtgccgacta agatcgacat cttcattacc ggcctgatta tcggcagcgg
      601 aacctatcca gttcattcgc tagtcggcct gctgcaacaa ttcaaggacg tgctcgatag
      661 cactcgccaa gcgccgaaac cgatcaggtc caagtagtga gctatcgata gggagaactt
      721 ttaatggccg agatttcgcg taaagaactg atcctggctt tagtcagcgc gggcgatcat
      781 ccgcgcttgt ccggtctcga cttgtcgaca ctcgatctgt cactgctcga tctgcatggt
      841 gcgcggatca ttttggccaa cctcatgcgc gctgatttgc gcggcgtcaa tttgagcgaa
      901 gcgaatctga ctgaatcgaa tctgatgcaa tcgaatctca gcggcgcgaa cttaagcaaa
      961 gcgattctga gctgcgtcga tctgacccag gccctgatcc gccgggcgat tctcaacggc
     1021 gctctgttgc gcagcgcaaa tctcaagaat gcgatcctca ttgaagccag tttggtcggc
     1081 gccgatctca gccaagccaa tttgcaccgc gccgatctca gccatgccaa tctgagcgaa
     1141 gcgaatctga ctaaagccga cctgagcgaa gcctctttag aaggcgccac tctcagccag
     1201 gccatcctca ccggagtaag cctgcatcgg gccaatttag ttggcatcaa tatgagcggg
     1261 gccaatctat ctggtatcga tgccagtcag gcagctctat ccaaatcaga tctcagtcag
     1321 gctaatctca cgagggcgaa tctaggcgga gctgatctca gcctagccga tctcatcgaa
     1381 gccaatgcgc acttcgtgaa tctgcgcagc gcgcatttag tccgcacgaa tctgcacggc
     1441 gcgctattac acggcgccac aattcaacag gccgatctga gtggtgccga tttatccggc
     1501 acagatctca gcaacgccga tctcacggat gcaaacctca gcggcgcgaa tctgactaaa
     1561 gcaaatctca agggcgcgcg cctgtacgga gcgcagttgt tcaaagcgaa cttgagtaat
     1621 gcgcttctgg aggaagcgat cttgaccaag gccgatttga ccggcgcaaa tctgaaaggt
     1681 gcagtcttaa ccgacgccaa tctggatcaa atcaatttgg acaaagcctt gctgcgcgat
     1741 gcggtgctgc gcgaatctaa agtgaatgcc aggcagtttt acaaagcatc tatgagctaa
     1801 atcaccacgc gcttgcatcg agcagctgtg caggcgtagc gtgtgtttag gccgtaaccg
     1861 gtcaacttta tcaaggtgtt ttcatccatg ggcggcttct tactccatgt cccgtataaa
     1921 tcacagaacg atgccgacgc aacgctcaag cgcagcgatt gtggtccggc gtgcgtcgcg
     1981 atgatcctga acgccagcgg ccagaacgtc acgaccaatc aggtgaccaa agcgagcggc
     2041 gtgatcggcg atcaaggcgt gcggatcaat caattaaaga tcgcggccaa agtattcgcg
     2101 ctggagctga acagccgatc gggtctcacg ctcgacgacc tcaaagccgc gctcaataac
     2161 ggccaaccgc cgatcgcctt gatcaattac ggtctgatcc ccgatcgctg ggatcgcaat
     2221 tactccggcg gacatttcgt cgtcctggtc ggctatgacg atgcagccgg ccgcgtcttc
     2281 atcaacgatc ctgattatcc gcccggcgcg cagggttatc agcggcctta cgcgttcgat
     2341 gttttttcgc gcgcgtggag cggctttgcg cgcaaccaga atccgaacaa ctgcctgatc
     2401 gttccgcagc tcacccgatc gatctccggc actccgatcg gaactccacc accctcaccc
     2461 aatggatcag ccgtgcatgc ctgggtgact atgccgcacg gccttccctt ttggtcacag
     2521 ccgaatacac actcccaatt gatcaacgat ctcgtttacg cgcagcatgt aagtcagatc
     2581 gactccactt cgggttggct gcaagtgatc accgactcag gcctgagtgg ttgggttgcc
     2641 gatcgattca acaactcgcc gacattgtcg aacacccagc caccagccgg ttatccagtt
     2701 gaagtgcttg acaccttgcc catccgcgct gtcggcggct ggagtgtacg cgcgcagcgt
     2761 aatctcaacg cgccgcgggc cgatcggatt gccgctggcg aacatgcgac ggtgtatatg
     2821 tatttcatgg acacggataa cacaccctgg ctgctcgtcc aatcacctcg cggacaatcg
     2881 ggctggggtc gtgaatcgac cggcagtatc gcgctggtgc gcacgatcga tctgcacacg
     2941 aaagacgaca cctcaacgac cacaccgggt acacctcctg cgccgaatcc tgcgccgatc
     3001 cactcaagcg atgtgtgggt cacggcgatc gatggtctag tgctgcgcga aaatcctgat
     3061 ccgtccgcac acaagatcgc cagtttgcct tttggtcaac gcttgatcgc tatcggaccg
     3121 gaatcgccca gagataacac ggggcgcgcg tggcagcccg tccgcatcga gaatggatcg
     3181 accggctttg tcgtagccgg cttcggcgcg gaacgctatc tttcgctgac ccggccgccc
     3241 aaaccctaca cgctggaagt gttggatacg caattcgcgc gcagccaagc tggcttgagc
     3301 attctgcaaa gccatgagga ttcagcgccc gtcatcgatc gcgcgcagat cggtgaacat
     3361 ctcaccgcgt acaatcggtt caccgataat accggctcct ggctatggct tgaatcgccg
     3421 cgcaacaaag ccggttggct gcgcgataaa gtcgacggcg cgatcttggt caagccgatc
     3481 gatcccggcc cgactcctca cccgatcgtc gatccacgcc cgttcggaaa atgtttaacc
     3541 ggcctgggca tggctaaccc tcaacctctc acgcagttgg aattcgatct gatcggcaag
     3601 agcaagatcg aagccttcaa aatcttgacg ctgcccgatc ccaacgatac ggtgcaactc
     3661 attcgaaccc tcaaaaacat tccgacgatc aagttcatcg tcgcccgctt atttttctcg
     3721 gtcgatgccg aaaacaaaac tcgtttctcg ccgcaaacat tcgtggagac cgtgtttcaa
     3781 agcgccagtg cggcctatca agccggcctg cgatattttg aaatccacaa cgaaccgaat
     3841 ctcgaaaacg aaggcttgaa ttggaattgg cgcaacggtg cagagttcgg agcatggttg
     3901 tcccaagtca tgtcgttctt gcgtgcgcgc cttccggaag cgcagctagg ttttcccggc
     3961 ctctcgccgc agccgaatgc gcctgacttt ctcaacggcg cacaatccgc actccgccaa
     4021 tgtgattggc tgggtgcgca cagctattgg caatcgatcg aaggcggtcc atatcccatg
     4081 gcagcctcga atgccggtat gtattggcga tcgatccgcg atcaatttcc gaataagtta
     4141 gtgatgatta ccgaattcag caacaataac agcggtgttg atctcgaaga gaagggccgg
     4201 caatacacgc gctactacca attgctgcgc aacgaaatga atctaggcgc agctttcagc
     4261 ttcgcgttga attggcccgg tcaggatgcc aatcacgagg gatgggaata tgatggcagg
     4321 gaaacgacca tcccgggcat cgtgggcagc gtgttgggtc aagcgggttt tttaggataa
     4381 ctttattcgg tatgagctgc cgccgcgcag ccggccaatg tggaagtcaa agaatcagcc
     4441 gtaaaaaagt tggctttgag atgtcattaa caggttacaa ccattcaact tcgagaccgg
     4501 caaattgaat atattctttg aacagcggct cggggttgat gagatcagac ggaaaggtag
     4561 tctctttgtt gcgggtcgcg ccgaccttgc agatcgatat atgcaaatgc gcgccgcgcg
     4621 cattgccggt ggcgccgccg gtgccaatga tggtgccggg atgaaccggc gagccgacga
     4681 tcaagtcgca ggattgatcg accatgtgcg cataaataaa ttcatattcg cggccctgat
     4741 attcaaggtg aacccggact tgataaccgt acggatcctt cggcgtgcga taaccaatga
     4801 aagtgatctg gccttccgta cagcaataga tcggcgacac ggccgcgcga gtttggacgc
     4861 gcacatccag gccttcatgt ccgggcaagc cgtactgctt atagtacgcc ggatcggttc
     4921 cgaagcgatt ggtaatgacc gtatattgag tcggccaata ttgaatgagc atcaagctcc
     4981 taagcgtcga tcgcctgtgc aggttgcgac gctattgtaa atcgatttga taaagttggg
     5041 aaaccgatcg ggcattgcgt acagcccgac ctgagctgga tgaatcgcac ccattgaaga
     5101 tcgatcatgt tgatcaccta aggggaacaa actcatggaa agttcaacgc tcataaacat
     5161 cgggtttggc ttggtgctgg gcttcttaat cggttggctg gtagaatggc ggctggatgt
     5221 gtcgttttgg aattcacaca ttgcccaact caaccgacgc gaggctgccc gccgcacgca
     5281 ggccgataaa gaaatagaag aagcgcaaat tgaggcgggg cagcaagtgg cggacctgcg
     5341 ccgccagctt gaagcggaaa ttgccgaatt gcgcgcccga tcagaagaac gggtagctgc
     5401 cgctcgagct aagacagaaa ctgaacccag tcgcattcga taaacattct ccgaaatatt
     5461 ttcgccgctt ctacaatcgg accatccatg ttacaatgcc tgcattgaag aggcatgatc
     5521 ttacatgttc catagcattg gcgacagact acttaacaat actgtaacgc gatagaatgg
     5581 tgagtaatct ccattttagt ttacactatt cactgataga accatatcag ctgaagaaaa
     5641 actaaattag gagattctca tggagctcaa acgtaaagca atcagtttag aattaagccg
     5701 cagctatctc atagcgcttg aagagctata cggacaacgt ctagccggca aactacagat
     5761 cgccctgatc gccagtttca cattgatcct catggtcggt ttgatggttg ccacacacag
     5821 ctttggatag cgccggccaa ttgaggagcc tgggtggcag atacggatcc caattcatca
     5881 gcgcgaaacg tctggtcaac ggtcaatgga tcatatcgag gccacattcg gcgcaagcgg
     5941 atcgtcgccg cggttatcat tttacttctg ctggctccgc ttacctatct ggtcgttaat
     6001 cagatcattc aagcccgagc gcagtcacct gatccagtca gcgtaagtgc ggttgaagtt
     6061 acaactacaa gcactttgag ccaaggcagc ggcgtgatag acacgccgac cctggctcaa
     6121 tttgtttcgg tcactccaag ggtgctgccc agcgaaacag ccaccgctgt acgcgtggtt
     6181 gatacatcaa ccgccgctgc accaacgaca ccacagccaa cacgaacctc aatccagact
     6241 caaattccac ggctgaccac tactgcgaca acttctgcac agaatgtcgc ggccagcaca
     6301 tcgacttcaa cacccaggcc gacaaacact ctgcagccaa ccgccacttc aatcgcggcg
     6361 gcccagagcg tctcgccaac gccgactgca acaccgaccg ctcaaccaac gctacagatt
     6421 gtaattccag cgacgggcgg tgagacgccg ggactgaatc tgtttcagat actggggttg
     6481 atcttgttgg gcatggtgtt gatcttgttg ggcagcgcgc tgatcgcacc gcccgcgcgt
     6541 cgataggtca cagcttcacg gtctcactca cgaccgccgt tgataccacg catacaatgc
     6601 gactaaaaac gatgagaccg tcgttgcccc aaacaatatc tgtccaacat tgcggattcc
     6661 ttcccacagc ggatcttcca attgaattgt aaattccaga ttcgtagccg gatacggttg
     6721 gccgttctgc gcagaatctc gaacttgagg ggttattaca atttgccgct tggctaagac
     6781 atcacgtcga ggcagcataa tataaaactg atagggcaag ccagttgtgt tggcggtgtt
     6841 gaggttgaag ctagggctga gggcatggca gttgttatcg ccgacaaaca gatccgcgtg
     6901 attatataga aacgaaatct caatcgggtt gacgggcgga ttcggtgtgc cgaaaaccgt
     6961 aatggtcacc ccggcagtgc aagcgcccac attatcgatc gtcaaccagc gcggataagt
     7021 gatctgaatc tgcgtagcac ctaaaccata attctccaac gatttcattg gcgtggtatc
     7081 caggcccgcg tgactattat ttgaaaagaa ccataacccc gccaggacta agccactcat
     7141 cagtccggcc acacgcacga acatttttcg atcagtcgtc acccacgtaa cggccagtag
     7201 cgccaagccc aacagcacta ccgttatcac aatgaaataa gcacctgcga gctgggtcag
     7261 ccacgaccag cgcagcgcca ccaagataat gacggccagc cagacggata tggcgcggct
     7321 ccattccggt agaccgctct caaaattatc tttggctgca tagctgctat gctccaactc
     7381 gatcatcgcg gtgcggagca attgcaccat ccgcagctgc aaggcccgtt tgctttgctg
     7441 tggcagagct tcgatcaaat gatcgaccag ctcctgcgag atcggcagtt gttctaagta
     7501 ctgaatcgcc aactctcgat cggcgtcttt cggcgcggtc agctgcgcta aatacgcctg
     7561 atagatctgc cgctcagcca gtggagcatc gccgcgatca taagccacgg ccacgcgacg
     7621 gggatagaca tagcgcagat aaacttgcgt cggcacgccg ctgcggaccg cccggcgcac
     7681 ggcctgccgc atcaggaaat aggccgcaat gcccaaaccg acgaacaaca aaatttgatc
     7741 aatgctcatc atgccaccag ttacaaccga atcgcatccg gcgcatcact gttgatgccg
     7801 agtagtctga acacctctgt ttttacttga gacggcagca ggccgcgctt ggggagatag
     7861 ccagccaagc cgaggctgtc cagtttgtaa gcctgggccg aattccgcac caccgtcaac
     7921 agcaaaatcg gacgcgtgac tttcagttcc actaacagcc atttcaagat gcccacaccc
     7981 acgtgttctt ctttctgcca cgcggaaact ttttcaggca ccggcttatt gtcattgcta
     8041 atgggcagaa tcaaatcgac gacgatcaaa tcatactgct cccactgcag cgccttgttg
     8101 aaaccgtcga ttgccgacgt cgccgcgtcc acctcaaatc cggctttctc caatggacgc
     8161 accaggccct taatcgcgta ataatcatct tcgatccaca gaattcgctt gttcatctta
     8221 aactcctcac atcacagcga aaaacttgcg gctcatgatc tggcaaaact gatcattgca
     8281 cattatttat tgcttgggtt gtcgcacggg cagtgtcacc gtaaaagtgg tgagatgagc
     8341 ttcaccaact accggcttgc tcgtcacgcc gatcgaacca tgatgcatct cttcaataat
     8401 ctgccgcacc tgcgccagac ccagacccgc accggtccgg cgtcgatcag acgccttcgc
     8461 gccgcgatag tacggcctga agatcagccg gctgtcgatc tcttctggtg gaatacccac
     8521 gccgtaattc tcgatattga tcgcgtaagc ctgccgatcg ggatcagccc actggccggt
     8581 aatgcgcaca tagcgctgtc ccgcatattt ctgcggcggc ttgaatgaat acttgatggc
     8641 gttatgaatc agattcttga acgcgatcgt gagatcaaac agcgacattt cgatatcagg
     8701 aaaccgcgaa ccaatcgcgc gcggatccaa aatatcgcag cctttcgccg ccgcttcgct
     8761 ttcaaacatc tgacacgcct ctttcaacgg ccggtacaga ctgtggatcg tcagcgcccg
     8821 ttccggcgct tggccggaca acaccgtcat gatcgtttcc gtcaacaagt gcagcttctg
     8881 cacttgttcc agcgtatggc cggccaattg cttcaactcg gagccagccg gcaattcctc
     8941 ttttagattt tccgtatccg ccatcacacc ctgcagcggc gtattgatct cgtgcgccag
     9001 cgacatgagc aatcgttttt gatcgtcctg cgtttttaga ttgcgcatga tcgccgctaa
     9061 ccgtccggcg atcgattcga ccagcgctac atcttcagca aagaacgggc tttgatcgat
     9121 cggccggccg atcgtgatga cgccaaacaa ctcagccgca tcgatctgga tcggcacggc
     9181 gatgaatgac caggacgctt tcgatccggc gcgatcgaat tccgcctttt tcgcggtggg
     9241 caaaccctta actcgatcgg ccagcagctg ctgagcgagc tcggtaaact tttgttggac
     9301 atgcgcagca ctggacaggc cgcgccgcaa ttgaatgaga cccaccaact gtttttgatc
     9361 gtcgagcaaa ttctcaatca agtcacccga ctgacgtgcc tgaagccgcg tgcgttccgc
     9421 ctttaaggca atctgcgagg cagcacccac gccaaagtga ttgacgacca gcgttgcgcg
     9481 agcgagcgca cagaaaccag tcttgccctc gccatattga taagtgatgg ccttcgctcg
     9541 atcggcgtca cttaggcccg cccggctgga acgtgccaag cgcaagcacg gcgcatcgac
     9601 gttttccgcc agataaatac tgcagcctgt gccggccacc agcaaaggca cttgttgaac
     9661 gatcagatca aacaacttat ccaagtcggc ttgaaagcca acttgcatga gcaagtcacc
     9721 cagccgccgt gcgcgatcgt tgttgatcgc cagtgcgatg gcgctggcaa atgcttgagc
     9781 gagctgcacc tcgtcacggt cgaacgagcg ttgacgcagt tggctcgcag cgctcagcgc
     9841 ctgcgcacac agcactccta aaatcgatcc atcatgagtc ttgaccggca cgcctaaaaa
     9901 gggcagaggc gcagtgctga gatcagccac ctcaatcaaa tctgatttca agaaacgatc
     9961 gtcgtcattg atcacggcgc cgtcggaaat ccgatcgaga tcaacgtcgg ccaatctttc
    10021 gccgtgcatg aaagcttgcg catccggcag cgacagccgt ttgcctgttt taaaaaccca
    10081 gccgatgaga tcatttccgc gcgcaatgga tgtttcttga tcaacgcgcc ggccattctc
    10141 gacaattaaa cgaacctcgc tgccgccttt ggcccgcaaa taaagttggc tgtgtgcgcc
    10201 cagcatgctg ctcaaccgat cggtgacggc ttcaaataat tgctgtgcgt cctgcttggc
    10261 gcccatctcc accagctgcc ggatgtgaac gctctgctgc tgaataattt tcgcctggtc
    10321 tatggcattg gcgaggaagc gcgcggcgat cacggccagg tcttcgtcgg tacgatcgaa
    10381 cggctgttga tcgatcttcg ccatgaactt gatcacgccc aacgtcccgc ggccggcgat
    10441 caacggcacg atcatgatcg gcttcgcttc gctcgctcga taaaacgatc gggcctcgcc
    10501 ataacgatcg agccatttca aatcaccagc ga
//
LOCUS       DASRTJ010000130         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7650,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000130 DASRTJ010000000
VERSION     DASRTJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7650"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFF70_02720"
     CDS             <1..773
                     /locus_tag="VFF70_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY43638.1"
                     /translation="LNRETFRAFANYVTSAEAQQTILSSGYRPADLSIKLDASGSPIN
                     SKNGVDPAEPQTALQVPSPAILDVVRQSWWATKRRTNVELVVDTSGSMEGEKLDNVKV
                     ALRTFIDQIKSDSEHMGLIQFYTNVDEVVKINDLKYTRSALISSIDSLEAGGDTALLD
                     GISAAYDRLQRLNDPERINAIVVMTDGKENRSNISLTRLISKLRGGSVPVVVFAIAYG
                     GDADMGYLQQVANATNGQTYQGTIETIRQLYKILSTYF"
     gene            915..2276
                     /locus_tag="VFF70_02725"
     CDS             915..2276
                     /locus_tag="VFF70_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43639.1"
                     /translation="MSSEEISTWLDQGLAAAASGDRVFARQQLQRVIEADDHNLQAWQ
                     ALSEIAATLEDREVCLENVLALNSNNSQAREQLDLVRAQIADVSEAEVKEVFSPMGLS
                     ASLSQPIRIDFADDHLDDPLLCVYCGHLTQEHDLRCPKCQRGQYQSFYKHEHARWIVL
                     GWIINIVDMFYTIGVLGVLLFFISTALSATKLVGQGDLGQLLLLYSGQATTLSPQAQA
                     AVLTALPRDFVYFRLGYSLFMLIVAFGLLTRRRVFHLLFIASVAAATAAVFLNYNFSN
                     TILAAGDASAATPFERILSVAIFEASNVSTVIIGAIAVVLLMIRLVVLFLVTDDFEKI
                     TERLWNQIDRSANNSRTAFVRAKAYMQREMWTLAARYLQQAVTLDSTTSDYFLALAES
                     YAHLERYPRALDMLDQADQLPTNSAATPHLRGVILQLQQRAAQASEKLSPPSPAVEEN
                     VDA"
     gene            2273..>2765
                     /locus_tag="VFF70_02730"
     CDS             2273..>2765
                     /locus_tag="VFF70_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43640.1"
                     /translation="MTIDRPEADLHQRVQHAMLSNAIFDWRGGIVIALAILLGFFIPE
                     PLPGFQWWYWLIGGAAAWLGLTASILTDPSTGARVTAELLQAKYEPTRLRNGDARDKM
                     IKAFEYRGRIAEAIEHSQPGVLRDHLKETSNQIDDWIANLFSVAKRLDAFEGDRTIQQ
                     DVRS"
BASE COUNT          616 a          707 c          805 g          637 t
ORIGIN      
        1 atctcaatcg cgaaacattc cgcgcgtttg cgaattacgt gacctcagca gaagcgcagc
       61 aaacgatttt gtccagcggc tatcgaccag ccgatctgtc gatcaagctc gatgcgtccg
      121 gctcgccgat caattccaag aacggcgtcg atccagcgga gccgcagacg gcattgcaag
      181 taccgagtcc cgccatcttg gatgtggtgc ggcaatcgtg gtgggcgacc aagcgccgca
      241 ccaatgtcga attggtcgtg gatacatcgg gcagcatgga aggcgagaag ctcgacaatg
      301 tgaaagttgc gctgcgcacg ttcatcgatc agatcaagag tgatagtgag catatgggac
      361 tgattcagtt ctataccaac gtagacgaag tggtgaagat caatgatctg aaatataccc
      421 gcagcgcttt gatcagttcg atcgactcat tagaagcggg tggcgatacg gcgctgctcg
      481 acgggatttc ggcggcttac gatcgattac agcggcttaa cgatccggag cgcatcaatg
      541 caatcgtcgt gatgacggac ggcaaagaga ataggtccaa tatttcgctc actcgtttga
      601 tcagcaaact gcgcgggggc tcagtgccgg tggtcgtgtt cgccatcgct tatggtggtg
      661 acgcggatat gggatactta cagcaagttg ccaatgcgac caacggccag acatatcagg
      721 gcacgatcga aacgattcgg cagttgtata agatactgtc gacatatttt tagtaacgcg
      781 tgaaaaataa tgagtaaaga gtaatgcata agtcgtgttg cggatcgcgt ggtgcgtgaa
      841 caaacggaac acgcaacacg caccacgttc aatcgacgat gagcgatata caataggcta
      901 tcagcgacaa gtatatgagt agcgaagaaa tctctacttg gcttgatcaa ggtttggcgg
      961 cggcagcgtc cggcgatcga gtttttgcgc gccaacagtt gcagcgcgtc atcgaggcgg
     1021 acgatcacaa tttacaggca tggcaagcgt tgagcgaaat tgccgccacg ctggaagatc
     1081 gagaagtgtg cctggaaaat gtgttggcgc ttaattccaa caattcacag gcccgcgaac
     1141 aactggattt ggtgcgggcg cagatcgccg acgtgtccga ggcggaagtc aaggaagttt
     1201 tctcacccat ggggctctcg gcctcgctgt cacagccgat taggatcgat tttgccgatg
     1261 accaccttga cgatccattg ttgtgcgttt actgcgggca cttaactcaa gagcacgatc
     1321 tgcgctgtcc gaagtgtcag cgcggccagt atcaatcatt ttacaaacat gaacacgcgc
     1381 gctggatcgt tctcggttgg atcatcaaca tcgtcgatat gttttatacg atcggtgtgt
     1441 taggggttct gttgtttttt atatcgaccg cgttgtcggc gacgaagctc gtcggacagg
     1501 gcgacctggg gcaactgttg ttgttgtata gcggccaggc gaccacgtta tcgccgcagg
     1561 cccaggcggc agtcttaact gcgctgccgc gcgacttcgt ttatttccgg ctcggctaca
     1621 gtctgttcat gttgatcgtt gcgtttggct tgttgacgcg ccggcgcgtc tttcacttgc
     1681 tgttcatcgc cagtgtggcg gcggcgaccg cggccgtctt cttgaattac aacttcagca
     1741 acacgatcct ggcggccggt gatgcttctg cggccacgcc ctttgagcgg attttgagtg
     1801 tggccatctt tgaagcatcc aacgtttcga cggtgatcat cggcgcgatc gcagtcgtgt
     1861 tattgatgat ccggctggtc gtgctgttct tggtgaccga tgatttcgag aagatcactg
     1921 aacggctgtg gaaccagatc gatcgatcgg ctaataattc gcgcacggct tttgttcgag
     1981 ccaaagcgta tatgcagcgc gagatgtgga cgctggcggc gcggtatttg cagcaagcgg
     2041 tcactttaga ttccactacg tccgattatt tccttgcgct ggccgaatct tacgcacacc
     2101 tggagcggta tccgcgggcg ctggacatgc tggatcaagc cgatcaattg ccaacaaatt
     2161 cagccgccac gccgcacttg cgcggagtga tcttgcagct gcagcagcgg gccgctcaag
     2221 cgtcggaaaa attgagcccg cccagtccag cggttgaaga gaatgtagac gcatgacgat
     2281 cgatcgacct gaagcggatc tgcatcaacg cgttcaacac gcgatgttga gcaacgccat
     2341 cttcgattgg cgcggcggca tcgtcatcgc tttggctatc ctgttgggtt tctttatacc
     2401 ggagccgctg cccggttttc aatggtggta ctggctgatc ggcggcgcgg ccgcgtggct
     2461 gggcttaacg gccagcatct tgaccgatcc ctcgaccggc gcgcgcgtca ccgccgaatt
     2521 actgcaagcc aaatatgagc cgacgcgcct gcgcaatggc gacgcgcgcg ataaaatgat
     2581 caaagcgttt gaatatcgcg gccgcatcgc tgaagcgatt gagcacagcc agcccggcgt
     2641 gctgcgcgat cacttaaagg aaacgtcgaa tcaaatcgat gattggatcg ccaacctgtt
     2701 ttcggtggcg aagcgactgg atgcttttga aggtgatcga acgattcagc aggatgtgcg
     2761 atccg
//
LOCUS       DASRTJ010000131         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7669,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000131 DASRTJ010000000
VERSION     DASRTJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7669"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFF70_02735"
     CDS             <1..299
                     /locus_tag="VFF70_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43641.1"
                     /translation="VILLEDRWSILNNNRTARQDPDLANYYALLAALDAGWEVDAPVY
                     LRADWSLARKDSMVFHFVLVLRSRRMTTLLSVPEGKAICRLIEENGWTISPPKP"
     gene            complement(322..1890)
                     /gene="nadB"
                     /locus_tag="VFF70_02740"
     CDS             complement(322..1890)
                     /gene="nadB"
                     /locus_tag="VFF70_02740"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZY43642.1"
                     /translation="MDELIETDILIIGCGIAGGSAALQLAEAGLHVTIVTRSIEPGES
                     NTYYAQGGVIYRGVADSPELLAKDILQAGAGYCNPSAVKILAEEGPSLVKQTLIDQLG
                     VQFDRTDGGNLALALEGGHSIPRIVHAADATGKSIELALMRALKAQLNITLLGGHTAI
                     DLLTPSHHSINRLAIYNPQSCVGAYLLDQSSGQVVRCLARYTILASGGLGQLFLHNTN
                     PIGARGDGIAMAYRAGARVINMEFVQFHPTTFYHPQAPRFLISEAVRGEGARLVHANG
                     EPFMQNYDAEWKDLAPRDVVSRSIYHEMLTRDVRNVYLDLRSYMSGDHIHEHFPNIYA
                     FCTGYGIDITRDLVPVVPGAHYSCGGIWVDEWGRSTLDRLYAIGEVSCTGLHGANRLA
                     STSLLEGLVWGHRSACDIRDHFAERTASKASDIPVWQVHGLKLPDPALIQQDMNTIKQ
                     MMWNYVGLVRTTDRLERALRELRHLETEIDRFYRVSILTDDLIGLRNAVRTSIIVAMA
                     AWENKASVGCHYRE"
     gene            complement(2052..>2859)
                     /gene="nadA"
                     /locus_tag="VFF70_02745"
     CDS             complement(2052..>2859)
                     /gene="nadA"
                     /locus_tag="VFF70_02745"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZY43643.1"
                     /translation="FCGVRFMAETAKLLNPSKTVLIPSLKAGCSLAASITAEDVRNLR
                     KQFPGVPVVTYVNTYADVKAESDICCTSGNAIAVVESLHAETVIFLPDEYLAGNVAKE
                     TGKHIIFPTKNPKATYADLDYQMIGWHGRCEVHEKFTVQDIRNVREQFPDVVILSHPE
                     CSPEVVTASDFSGSTTAMIKYVEQTHAPRYLLLTECSMGDNIMAANPDKEMLRLCSVR
                     CPHMNQITLEDTREALLKTQYVVEVPADIRERAARAVQRMIQIGGVGKKD"
BASE COUNT          640 a          816 c          753 g          650 t
ORIGIN      
        1 atgtaattct gctcgaagat cgctggtcga tcttgaacaa caatcgcacc gcccgtcaag
       61 atccagattt ggcgaattac tacgccctgc tggccgcgct ggatgccggc tgggaagtcg
      121 atgccccggt gtacctgcgc gccgattgga gtttggcgcg taaagattca atggtctttc
      181 actttgtatt agtgttgcgt tcacggcgca tgacgacgct gctcagtgtc ccagagggta
      241 aagcgatctg ccgcctgatt gaagaaaatg gttggacgat cagtccaccc aaaccgtaat
      301 cggcgttgaa tcctttcaaa actattcccg ataatgacag cccacactcg ctttattttc
      361 ccacgcggcc atggccacga tgatcgaagt ccgcaccgcg ttgcgcaggc cgatcaaatc
      421 atcggtcaag atcgacaccc gatagaagcg atcgatctcg gtttcgagat gacgcagttc
      481 tctgagcgcg cgctctaatc gatcggtagt gcggaccagc ccgacatagt tccacatcat
      541 ctgtttgatc gtattcatat cttgttggat cagggcggga tcaggcagtt tgagaccgtg
      601 cacttgccaa accggaatat cactcgcctt ggatgcagtc cgctccgcaa agtgatcacg
      661 aatgtcgcac gccgatcgat gtccccacac cagaccttcc aacaacgacg tgctcgccaa
      721 tcgattcgcg ccatgcaggc cggtgcaaga cacttcgccg atcgcataca accgatcgag
      781 cgtggagcgg ccccactcat ccacccagat gccgccacat gaataatgcg ccccgggcac
      841 taccggcacc agatcgcgcg taatgtcgat gccgtaacct gtgcagaacg catagatatt
      901 tggaaagtgt tcgtgaatgt gatcgcccga catatatgag cgcagatcaa gatagacatt
      961 gcgcacatcg cgcgtcagca tctcatgata gatgctgcgc gagaccacat cgcgcggcgc
     1021 aaggtctttc cattcggcat cgtagttttg catgaacggt tcgccattgg cgtgcacgag
     1081 ccgcgcgcct tcgccgcgca ccgcttcgga gatcaagaag cgcggcgcct gcgggtggta
     1141 aaacgtcgtg ggatgaaact gcacgaactc catgttgatc acgcgcgcgc ccgcccgata
     1201 ggccatggca atcccatcgc cgcgcgcgcc gatcgggttc gtattgtgta aaaaaagttg
     1261 tcccaacccg cctgaggcca agatcgtata acgcgccagg cagcgaacga cttggccgct
     1321 tgattgatcg agtaaatacg cgcccacgca agactgtgga ttgtagatcg ccaatcgatt
     1381 gatcgaatga tgggatggcg tgagcagatc gatcgcggtg tggccgccga gcagcgtaat
     1441 gttcagctga gcttttagcg cgcgcatcaa cgccagctcg atcgatttgc cggtcgcatc
     1501 ggccgcatgc acaatgcgcg ggatcgagtg gccgccttcc agcgccagcg ctagatttcc
     1561 accatccgtt cgatcgaact gcacgccgag ttgatcgatc aatgtttgct tgacgagact
     1621 cggtccttcc tccgctaata tcttcacggc gctcggattg cagtagcccg ctccggcctg
     1681 cagaatatct ttcgccagca gttctggcga atcagccaca ccgcgataaa tcactccgcc
     1741 ctgcgcgtaa tacgtgttcg attcgcccgg ttcgattgag cgcgtcacga tcgtgacgtg
     1801 tagacccgct tcggccaatt gcagcgcggc actcccgcct gcgatgccgc agccgatgat
     1861 caagatatcc gtttcgatca gttcatccat gattgcctcg aataaaacca cagagatttg
     1921 tggatcgtca ttgcgaggaa gccgcgaggc taacgaaaca atctcagaat cgatcgggag
     1981 attgcttcgc ttcgctcgca atgacgctgc ctaagctatt gttgtctctt catatttctg
     2041 tggttaatct tctagtcttt cttccccact ccaccgatct gaatcatccg ctggacggcg
     2101 cgtgcggcgc gctcgcggat gtctgccgga acttcaacca catattgtgt tttgagcaat
     2161 gcctcgcgcg tatcttcgag cgtgatctgg ttcatatgtg gacagcgcac gctgcacaag
     2221 cgcagcattt ctttatcggg gttggccgcc atgatgttgt cgcccatcga gcattcagtt
     2281 agcagcagat agcgcggcgc gtgcgtctgt tccacgtatt tgatcatcgc cgtggtactg
     2341 ccagagaagt ctgacgcggt gacgacttca ggactgcatt cgggatgaga caagatgacc
     2401 acatcgggaa actgttcgcg cacgttgcga atgtcctgca ccgtgaattt ttcgtgaact
     2461 tcacaacggc catgccaacc gatcatttga tagtcgagat cggcatacgt ggcttttgga
     2521 ttcttagtcg ggaaaatgat gtgcttgccg gtttctttcg cgacattgcc cgccagatat
     2581 tcatcgggca agaagatcac ggtctcggca tgcagcgatt caaccacagc gatagcattg
     2641 ccggacgtgc agcaaatatc agactcagct ttcacatcgg cataggtgtt cacgtacgtc
     2701 acaaccggca caccgggaaa ttgttttcgc agattgcgta catcttcagc agtgatgctg
     2761 gcggccaaag agcaacccgc cttcaacgaa ggaatcaata ccgtcttaga aggatttaaa
     2821 agtttcgcag tctcggccat gaaacgcaca ccgcaaaag
//
LOCUS       DASRTJ010000132         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_343927,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000132 DASRTJ010000000
VERSION     DASRTJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343927"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /gene="ssrA"
                     /locus_tag="VFF70_02750"
     tmRNA           complement(<1..349)
                     /gene="ssrA"
                     /locus_tag="VFF70_02750"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            470..1285
                     /locus_tag="VFF70_02755"
     CDS             470..1285
                     /locus_tag="VFF70_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteinyl-tRNA synthetase"
                     /protein_id="HZY43644.1"
                     /translation="MNPIDQLGTITFMSSGEMSARLSPAHRAVMSRLKVPIRAIFLDT
                     PAGFQENATALAEKAVGYFNQNLQKELSIVSFKSKADAAPDRIAQVAQQIEAANYIFA
                     GPGSPTYAVRQWIDTPIARAFAARLNNGGHLVFASSAAIAMSRWVVPVYELFKAGHDP
                     YWVDGLNLLGAFGVEVAVMPHWNNSEGGQDFDTRYCFIGRRRFKKMEDLLPDSAVMLG
                     LDENTACLLDLAAKSCEVFGAGRVILRHRGQEKIFASGTKFDWNEFESIKNDK"
     gene            complement(1357..2175)
                     /locus_tag="VFF70_02760"
     CDS             complement(1357..2175)
                     /locus_tag="VFF70_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43645.1"
                     /translation="MPRFAAPDGQPLTSIRSINDLPEIDHERIYGTLIPSDLLERYHI
                     SPIEFHSPTGDRLVEIDAPPGTGSIEIKVWHQAGARDPVLYFQLADTPNNQLIVLLFV
                     INDPEGPRFDIDRNWRGERTKFGTMNRNIEAEIEAMQAGLGPGQIRRGLHLSRKVQAT
                     FENFVAGLGHELFFFEPLAYHTAILFERYGCNYSVGKKKMEWIDHEFQAPDGELFKRL
                     DSSTPFRMSGAEKTVRGRSWAIHDGILGEPFTAIHMYRRIGIDSGINTFPSAEW"
     gene            complement(2175..2645)
                     /gene="smpB"
                     /locus_tag="VFF70_02765"
     CDS             complement(2175..2645)
                     /gene="smpB"
                     /locus_tag="VFF70_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZY43646.1"
                     /translation="MTRTSSSDIKIVATNRTATHNYSIEDRFEAGLVLRGTEIKSIRA
                     GQANLREGYVLLRHDELFLANTHISPYDPAGRGGHTPLRERKLLMHRKEIAKIAQKLN
                     ERGYTLIPLKIYLKNGRAKIEIALAKGKREYDKRQTIAERDAARQIDRDLKERR"
BASE COUNT          614 a          720 c          821 g          693 t
ORIGIN      
        1 gagtgatgac gaatgactac aggcttagtc gatgtttagt gtcgccgatc ggctctgcat
       61 cgacacggag atccaatcgg tcagccgaaa agtcttaggt tcgccctatc ggcatcaagt
      121 gaaccgcacg ccggcattgt cgcgcctgca tctcgaccca ccagcgcgag atcgaggcag
      181 acggatcatc cgaagacgat cgcggtctca ggttcgctta agcagcgagc gggagggccg
      241 catagttagt gcggttggca cttaaagttt gcttcattgt taacggaggt aaagcgctcc
      301 ggcttgccac gcgtcctcga ctgcttcccg tcgaagccag tgcatcccca gataagttga
      361 ttatacactg ccaaatgaaa aatgacgaat cacaaatggc ggatgatata atcaccagtg
      421 aaaactggcg gcgtgcgatt tgcgaaatac agccggtgag gagagtagtg tgaacccgat
      481 cgatcagctc ggcacgatta cgtttatgag ctccggcgaa atgtcggcgc gcttgagtcc
      541 ggcgcatcga gcggtgatga gccgcttaaa ggtaccgatc cgtgcgattt ttctggatac
      601 accggcgggg tttcaggaga atgcaacggc attagctgaa aaggcggtcg ggtatttcaa
      661 tcagaatctg caaaaggaac tctcgatcgt ttcgttcaag tcgaaagcgg atgccgcgcc
      721 tgatcggatt gcccaagtcg ctcagcaaat tgaagcggcg aattatatat ttgccgggcc
      781 gggcagtccg acgtacgccg tgcgccagtg gatcgatacg ccgatcgcgc gcgcgtttgc
      841 cgcccgatta aataacggcg gccatttagt ttttgcctct tcggcggcaa ttgccatgag
      901 ccgctgggtt gtgccagtct atgagttatt caaagcgggg cacgatccgt attgggtaga
      961 cggacttaat ttacttggcg cgtttggtgt ggaagtggcg gtcatgccgc actggaacaa
     1021 ttcggaaggc ggccaagatt tcgatacgcg ctactgtttc atcggcagac ggcgcttcaa
     1081 gaagatggaa gacctgttgc ccgattcggc cgtgatgttg gggcttgatg aaaacactgc
     1141 ctgtctgctc gatcttgcgg caaaatcgtg cgaagtgttt ggagcagggc gggttattct
     1201 gcggcatcgc ggacaagaaa aaatctttgc gtcaggcacg aagtttgatt ggaatgaatt
     1261 tgagtcaatt aaaaatgaca aatgacaaat gaaaaaataa cagaggtgct gattgtttgg
     1321 cgctgtgcag ccaatttcta acttccagca tctaattcac cattccgcac ttggaaacgt
     1381 attgatcccc gaatcgattc caatccgtcg atacatatgg atcgcggtaa atggctcgcc
     1441 caggatgccg tcgtgaatcg cccacgatcg accgcgtacc gttttttccg cgcccgacat
     1501 tcgaaacggt gttgaactat ccaagcgttt gaatagttct ccatccggcg cttgaaactc
     1561 gtgatcaatc cactccattt tctttttgcc gacgctgtag ttacagccgt agcgttcgaa
     1621 taggatggcc gtgtggtaag cgagcggctc gaagaaaaac aattcgtgcc ctaaaccggc
     1681 gacgaaattt tcgaaggttg cttggacttt gcgcgatagg tgcagtccgc gtcgaatttg
     1741 tcccggcccc agtccggcct gcatcgcttc gatctcggct tcgatgttgc ggttcatggt
     1801 gccgaacttg gtgcgctcgc cgcgccaatt gcgatcgata tcgaagcgcg gcccttccgg
     1861 atcgttgatc acaaacagca acacgatcaa ttggttgttg ggcgtgtcgg ccagttggaa
     1921 gtacaacacg ggatcgcgcg cgcccgcctg atgccacact ttgatttcga ttgagcctgt
     1981 gccaggtggt gcatcgatct cgactaatcg atcgccggtc gggctgtgga attcgatcgg
     2041 ggaaatgtgg taacgctcta agagatcgga cggaatcaat gtgccgtaga ttcgttcgtg
     2101 atcgatctcg ggcagatcat tgatcgagcg tattgaggtg agcggctgac cgtctggcgc
     2161 agcgaagcgt ggcattaacg ccgttccttg aggtctcgat cgatctggcg ggcggcatcg
     2221 cgctcggcga tggtctgccg cttgtcatac tcgcgtttgc ctttcgccag tgcaatttcg
     2281 atcttggcgc gaccgttctt gagataaatt ttcagaggga tgagcgtata accgcgttca
     2341 tttagttttt gtgcgatctt ggctatttct ttgcgatgca tcagcagctt gcgctcgcgc
     2401 agcggagtat gaccgccgcg gccggccgga tcgtagggcg agatgtgcgt gttcgccagg
     2461 aataattcgt cgtggcgcag cagcacgtag ccttcacgca ggttggcttg cccggcgcgg
     2521 atcgatttga tctccgtgcc gcgcaagacc agaccggcct cgaaacgatc ttcgatcgag
     2581 tagttgtgag tggccgtgcg attggtggcg acgatcttga tgtcgctgct tgaggttctg
     2641 gtcatgctaa tcacacagac ccgcgaagtt tccaaaacct cgcgggtctt tgagtgacgc
     2701 tatttcccgt taagcaaata atcgaccgcg cgatcgagct gcgggtcctt gaccgggtca
     2761 tcaccggcct cgactttgat atcgggctcg atgcctttct ggttaatatc cacgttattt
     2821 ggcgtaaacc agtgcgcgat cgtcaccc
//
LOCUS       DASRTJ010000133         6923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_310487,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000133 DASRTJ010000000
VERSION     DASRTJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310487"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="holA"
                     /locus_tag="VFF70_02770"
     CDS             complement(<1..662)
                     /gene="holA"
                     /locus_tag="VFF70_02770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZY43647.1"
                     /translation="MFYILHGENELERSEQVADFKRKIGDESVRDLNVTVLDGRKTTL
                     SELQHAADSIPFLADKRLVVVEGLLGRLAGRKPKTSDEAENDSAPTGSAKDFLNSLLS
                     FLPNVSESTRLVFVEAQSIKPTHPILKLAQKQTGKTVIEFPQPQVGELTNWIIKRAKK
                     HGGEVDRVAASKLAAMIGGDLRRLDQELEKLITYVNAQRIVTEKDVVLLVADSGLSNI
                     FD"
     gene            complement(746..1252)
                     /locus_tag="VFF70_02775"
     CDS             complement(746..1252)
                     /locus_tag="VFF70_02775"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HZY43648.1"
                     /translation="MDILDIPLIRRTRRNHALEHASIHLITAAYPGTPMAGRSTPYGF
                     YVYGNISQDMLTTAAYDALRRLRSGESKLAIHPGCGTNYLTSGIFAGSAAFATLSIGR
                     GRNRLTRLPDVFIAATLALILAQPIGPLLQEKVTTLADMGDLEITSVRPLGSRGTLKV
                     HYVETFSS"
     gene            complement(1270..2040)
                     /locus_tag="VFF70_02780"
     CDS             complement(1270..2040)
                     /locus_tag="VFF70_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZY43649.1"
                     /translation="MIDRSTGNSGAGCAANLLTIAGVLLLLAGGIAVYPSLRQSIEPT
                     PANFGEAAPPLRPTVTSIPALGQMPADVVVLPETPLLIPGQGLPEPIVIPTPLPDQVG
                     GSLPTRIVIAAIKLDAPITPVGLHQVDGNLTWDVPNYFAAGWLNRSALLGKPGNTVLD
                     GHHNILGLVFARLKDLKAGDRIQLYAGDKAFTYEITDMHNLLERDQPIAVRIQNAQWI
                     QSTTDERVTLITCWPPNNNTNRLILVAKPVKAQPFNIN"
     gene            complement(2121..2861)
                     /locus_tag="VFF70_02785"
     CDS             complement(2121..2861)
                     /locus_tag="VFF70_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43650.1"
                     /translation="MNLSAILSLISTVVSIIFAIILLRRYLTRKGRHLLVWGIALLIY
                     GTGTFAEFYSSQAWNATIFRLWYLCGATLAAAWIGQGTVYLLVRKPNIANILMGILLV
                     GTVIAAVQLFSTQLNEGIFKPEVPLSLQYATNSAKGIVGIMPEGGVRSYSFVFNIYGT
                     LALVGGALYSAYVFWRKRIMPQRVIGNVLIALGGLSPALGGTLARIGTPEFLYVSELI
                     GVIVIFIGFRLATTQQPVEAPLTQSVAA"
     gene            complement(2909..3085)
                     /locus_tag="VFF70_02790"
     CDS             complement(2909..3085)
                     /locus_tag="VFF70_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43651.1"
                     /translation="MSQSPDRAPAPPAAHKQTPRVPANFLYAKLVPIAIGVMTIVLLV
                     ILVVGIAGLLNTLK"
     gene            complement(3082..3708)
                     /locus_tag="VFF70_02795"
     CDS             complement(3082..3708)
                     /locus_tag="VFF70_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZY43652.1"
                     /translation="MADPSNDEWLSLRAASDLVGVHPATLRAWADRGHVASQRTAGGH
                     RRFRRSDIEHWARSRREPTASIEMLIQNAMGRVRLTLEQADMPWLTRLDAETRVQHRD
                     LGRRMLMEISTALDPAANSVTMISSAQRLGADYTRLSRDHGLSLAETVRTFMFFRDTI
                     ADSFIQLVSHLETAAAPDWIAMNRRLNEFTNAVLLGLIESYQRMEAAV"
     gene            3897..4553
                     /locus_tag="VFF70_02800"
     CDS             3897..4553
                     /locus_tag="VFF70_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43653.1"
                     /translation="MTATASNRFQTYIAISIAVATVLGAILAARATILNDTANQADQS
                     GLASAIDLALTRASDEAGRTQNLIAFLDFSQHRRLADLIVTDIEQLDSSNPAIEQLLD
                     QAETEWNKAIASRSFFDTDYYDKDSQAFDQQRFLDAHFAEVASKKDLEPEPHFNDAEA
                     TRAKAVKFVLMIAVLSFALFCFAVAGLFSSRWRYAAAGLGTIILIGTIALAIIIETAA
                     "
     gene            4563..6239
                     /locus_tag="VFF70_02805"
     CDS             4563..6239
                     /locus_tag="VFF70_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43654.1"
                     /translation="MSTEPDRSTQPDRTDQQFKRIVVTLMSIVTILSALTAFLQTASA
                     SRSSTAVRNSQRHALSQVETALQTQQQQNYDLYVYEQWSSLLAQRLVQLGSSRGISVT
                     SPAQHLSDLMQLTANFSPLMQPPYLTDPINGLLDLARYYADNEYQSTYWRQRREASAA
                     EGEIWGRKAAAYVMVLSLLAVALFLLGLAATIGGQLRGAFVLLGGFFAVIGTVWMLFT
                     AIIPVPVRTDLAMQTFARGHRNIVQGEILRGWDQITAAMPQLLSGIDEMNKALQLDPN
                     YGDALNERAFAYLIAAELQVFSGQDAHQLLSAAVADFQATVDLGRTDLTTLWNLGYAE
                     YLLGDYANALNWTERAIDRAPQQVGLYLNRAAALLGLGRRSEALTEVDHAVDQAVQQP
                     ISTAYYYFRQAIYDLTLLSKARPAMALADLLVYIKEKYVSLRYRHLAPPQAVDTQITN
                     LRFGSELNTNNDVLNFATTFPARTEKVFVAFDYAALPVGGEIDALVYRNDREDDTLSS
                     VGVTNLSGTGSAWLQIITPFINAGGLTSGHYRVDIYVAGKFLASAEFDIQ"
     gene            complement(6243..>6923)
                     /locus_tag="VFF70_02810"
     CDS             complement(6243..>6923)
                     /locus_tag="VFF70_02810"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZY43655.1"
                     /translation="LKELLPEARTLRWDRDTTGGKDSHAIILQHFINHEADVLIGTQM
                     IAKGLDLPLVTLVGVISADTTLHLPDFRSSERTFQVLAQVVGRAGRSILGGRAIIQTY
                     APDNYAIEAAAQHDYESFAKKELEFRQQTAYPPFTRLARLLIRDENADRVKREAEKVA
                     TQLDALLTRIGIARDSIIGPAPCFFAKLRDQYRWQIALRHPDPVSIVRELRLGPAWRI
                     DIDPLSML"
BASE COUNT         1573 a         1997 c         1833 g         1520 t
ORIGIN      
        1 atatcgaaga tattgcttaa gccagaatca gcgactaaca acacaacatc tttttcagtt
       61 acaattcgct gcgcgttgac gtaggtgatt aacttttcaa gttcttgatc gagccggcgc
      121 agatcaccgc cgatcatggc cgctaactta cttgccgcca cacgatcgac ctcgccgccg
      181 tgtttcttgg cgcgcttgat gatccagttg gttaactcac caacttgcgg ctgcggaaac
      241 tcgatcacgg ttttgccggt ttgcttctgc gcgagtttaa gaatggggtg agtgggcttg
      301 atcgattgtg cttcgacaaa gactaagcgc gtgctctctg atacattcgg cagaaaagac
      361 agcaggctat tcaaaaagtc tttggccgaa cccgtcgggg ccgaatcatt ctcggcttca
      421 tcactagtct ttggcttgcg cccggccaat cggccgagca acccttcgac aacgactaag
      481 cgcttatcgg ccaggaacgg gatcgaatca gcggcatgct gcagttcgct tagcgtggtc
      541 ttgcggccgt caaggacagt cacattaaga tcgcgcacgg actcgtcgcc gatcttgcgc
      601 ttgaagtcgg cgacttgttc gctgcgctca agttcgtttt cgccgtgaag aatgtagaac
      661 ataggaaagg cgaccacaga ccgcggaccg ccgttgatcg ctgttgacca agaatcagct
      721 gcggtcttcg gtcaccggtc tgcggtcagg aactaaacgt ttccacatag tgcactttca
      781 gtgtgccacg cgaaccaagc ggacgcaccg aagtaatttc gagatcgccc atgtcggcca
      841 gcgttgtaac cttctcttgc agcagcggac cgatcggttg agcgaggatc aacgccaacg
      901 tcgcggcgat gaacacatcc ggcaggcgtg tcaatcgatt gcgcccgcga ccaatgctca
      961 gtgttgcaaa ggcagcgctg cccgcaaaaa ttcccgatgt caaatagttt gtcccgcagc
     1021 ccggatgaat tgccaattta ctttcaccac tgcgcaagcg acggagggca tcatacgccg
     1081 ctgttgtcag catatcctgc gagatattgc catagacata gaaaccatat ggcgtcgatc
     1141 gaccggccat gggcgtgccg ggatatgcgg cagtgatcaa gtggatgctg gcatgttcca
     1201 gcgcgtgatt gcgccgcgtg cgtcgaatga ggggaatgtc taaaatatcc atagcctggc
     1261 ttctctctcc taattgatat tgaatggttg tgctttaacc ggtttggcga cgagaataag
     1321 tcgattggta ttattgttcg gcggccaaca cgtgatgagg gtgacccgct catcggtcgt
     1381 cgattggatc cactgcgcgt tttggatgcg gactgcgatc ggttgatctc gctctaaaag
     1441 attatgcata tcggtgattt cgtaagtgaa ggctttatcg cccgcataga gttgaatccg
     1501 atcgcccgct ttcaagtcct tcagcctggc aaacactaaa cctaaaatat tgtgatgtcc
     1561 atctagcacg gtgttacccg gcttgcccag cagcgccgat cggttcagcc aaccggccgc
     1621 aaaataattc ggcacatccc aggtgagatt gccgtcgact tgatgcagcc caaccggcgt
     1681 gatcggcgca tccaacttga tcgccgcaat cacgatccgc gtcggcaagc tgccgccaac
     1741 ctgatccggc agcggcgttg gaatgacaat cggctccggt aagccttgac cgggaatcag
     1801 cagtggagtt tcgggcagca ccacgacatc ggccggcatt tggcctaagg caggaatcga
     1861 cgtgacggtc ggccgcagcg gcggcgcagc ttcgccaaaa ttcgccgggg tgggctcgat
     1921 cgattgtcgc agcgatgggt acaccgcaat gccgcccgcg aggagcaaca gcacaccggc
     1981 aatcgtcaat aaattcgccg cgcaacccgc gcctgaattg ccggtggagc gatcgatcat
     2041 gcgatcgatt atacccgaaa agcaatcgtg cgacgcactg gatcagtgcg ccgcacgttg
     2101 agcatgcaaa atcaatcggc ttaagctgca accgattgag tgagcggcgc ttcaacgggc
     2161 tgctgcgtgg tcgccaatcg aaagccaatg aagatcacaa tgacgccgat gagctcactg
     2221 acatataaga attccggagt gccgatacgc gccaaagtgc cgcctagtgc aggcgacaat
     2281 ccgcccagtg cgatcagcac attgccaatc acgcgctgcg gcataatgcg tttccgccaa
     2341 aatacatatg ccgaatataa agcgccaccc accagcgcca gtgtcccgta aatgttgaag
     2401 acgaatgaat atgagcgcac accgccttct ggcattatgc cgacaatgcc cttggctgaa
     2461 ttggtagcat actgcaaact caaaggtact tctggcttga agatgccttc attaagctgt
     2521 gtcgagaaca actgcactgc cgcaatgacc gtgccgacta acagaattcc catcaagatg
     2581 tttgcgatgt tcggctttcg cactagcaag tacaccgtgc cttgaccgat ccaagctgcg
     2641 gcgagtgtcg caccacacag ataccacaac cggaagatag ttgcattcca tgcttgactg
     2701 gaataaaact cggcgaatgt gccggttccg tagatcagca gtgcgatgcc ccagactaac
     2761 aaatgtcgac ctttgcgcgt caaatatcgg cgcagcaata tgattgcaaa tatgatcgat
     2821 acaacagttg agattaacga caatatggca ctgagattca tgtttggctc cttgaacagg
     2881 ctaaatgatt ttgtataatc acaagatttc acttaagggt attgagaaga ccagcaatgc
     2941 ccactactaa aatgaccaga agaacaatgg tcatcactcc gatcgcaatc ggcaccagct
     3001 tagcatacag aaagtttgcc ggcacgcgcg gtgtctgttt gtgcgcggct ggcggagccg
     3061 gcgctcgatc gggtgattga ctcatacggc cgcttccatg cgttgataag attcgatcaa
     3121 tcctaacaac acggcgttag tgaattcatt caaacgccgg ttcatcgcaa tccaatcggg
     3181 tgcagccgcc gtctccagat ggctgaccaa ttggatgaag ctatcggcaa tcgtgtcccg
     3241 gaagaacatg aaggtgcgca ctgtttcggc caaggataaa ccgtgatcgc gcgacaagcg
     3301 cgtgtaatcc gcacccagcc gctgcgctga agaaatcatg gtcaccgaat tagccgccgg
     3361 atcgagcgcc gtgctgattt ccatcaacat gcgccggccc aagtcgcggt gctgcacacg
     3421 cgtctccgca tccaagcgcg tgagccacgg catgtcggcc tgttccagcg tcagccgcac
     3481 acggcccatg gcattttgga tcaacatctc aatgctggcc gtcggctcac gccgcgatcg
     3541 tgcccaatgc tcgatgtctg atctgcggaa gcgtcgatgt ccaccggccg tcctttggct
     3601 cgcaacgtgg ccccgatcag cccacgccct caacgtagcc ggatgcacac cgaccaaatc
     3661 ggacgctgcc cgtaaggata gccactcatc atttgaagga tctgccatgt tgtattccgc
     3721 caaaattcta tataaaccaa gatttaagag tcagattcta catctttaag gagatttgtc
     3781 aattgacaag aggcacacct gcgaggtgtc attcataacc tgctgcttag aaccctgttg
     3841 ctattttgcg ctgggctcac tataatgcga ccaacttgtc cctgcgagga gttcaaatga
     3901 ctgccaccgc gtcgaatcgc ttccaaactt acatcgccat cagcattgcc gttgcaaccg
     3961 tgctgggggc gatcctggcc gcgcgcgcaa caatcttgaa tgacactgcc aatcaagccg
     4021 atcaaagcgg cctcgccagc gcgatcgatc tggctttgac ccgcgccagc gatgaagcgg
     4081 ggcgaaccca aaatctgatc gcctttctcg atttttcgca gcatcgccgc ctggccgact
     4141 tgatcgtcac cgacatcgag cagctggact catcgaatcc ggccatcgag cagttgctgg
     4201 accaagcgga aaccgaatgg aacaaggcta tcgccagccg ctcgttcttc gacaccgatt
     4261 actacgacaa agattcgcaa gccttcgatc agcagcgctt tctcgatgcc catttcgccg
     4321 aagtcgcttc caagaaagat ctggaacccg agccgcactt caacgacgcc gaagccaccc
     4381 gggcgaaagc cgtgaagttt gtattgatga tcgccgtgct gtcctttgcg ctgttttgtt
     4441 tcgcggtggc cggtttattc agcagccgct ggcgttacgc cgcagccggt ctcggcacga
     4501 tcattctgat cggaacaatc gcgctcgcga tcatcatcga aaccgccgcc taggagcgtc
     4561 ccatgtctac tgaacccgat cgatccaccc agcccgatcg caccgatcag cagttcaagc
     4621 gaatcgtcgt cacgttgatg tcgatcgtca ccatcctttc agcgctcacg gcttttttgc
     4681 aaactgcctc ggcctcgcgc tcgagcaccg ccgtgcgcaa cagccagcgt catgccctca
     4741 gccaggtgga gaccgcgctg caaactcaac agcagcagaa ttacgatctt tacgtgtacg
     4801 agcagtggtc aagcctgctg gcccagcgcc tcgtgcagct gggatcatcg cgcggcatat
     4861 ccgtcacttc gccggcccag catctgtccg atctgatgca gctcacggcc aacttcagcc
     4921 ccttgatgca gccgccttat ttgaccgatc cgatcaacgg cctgctggat ctcgcccgct
     4981 attacgccga taatgaatat caatcgacgt actggcgtca acgccgtgaa gcttctgccg
     5041 cagagggcga aatctggggc cggaaagcgg ctgcgtatgt gatggtgttg tccctcttgg
     5101 ccgtcgcctt gttcttgttg ggtttagcgg cgacgatcgg cgggcagctg cgcggcgcct
     5161 ttgtcctcct gggtggattt ttcgccgtga tcggcacggt gtggatgctg ttcaccgcga
     5221 tcattcccgt gcccgttcga accgacctgg ccatgcagac tttcgcccga ggtcaccgca
     5281 acatcgtgca gggcgaaatc ctgcgcggct gggatcagat cactgcagcg atgccgcagc
     5341 tgctcagcgg tattgacgaa atgaataaag ccctgcaact cgatcccaac tatggcgatg
     5401 cgttgaatga acgggctttc gcttatctca tcgccgccga gctgcaagtc tttagcggac
     5461 aagacgcgca tcaactgctc agcgccgcgg tggccgattt tcaagccacg gttgatcttg
     5521 gccgtacgga tcttacaact ttgtggaatc tgggctatgc cgaatatttg ttgggcgact
     5581 acgcgaacgc actgaactgg accgagcgcg cgatcgatcg cgcgccgcag caggtaggcc
     5641 tatatctcaa tcgagcggcg gcgctgctgg ggttggggcg tcgcagtgag gcgctcactg
     5701 aagtcgatca cgcggttgac caggcagtgc agcaaccgat cagcaccgct tactattatt
     5761 tccgtcaggc catttatgat ctcacgctgc tgtcaaaagc gagaccggcc atggccctgg
     5821 ctgatctgct ggtgtatatc aaagaaaaat acgtttcgct gcgctaccgc catctcgcgc
     5881 cgccccaggc ggtcgatacg cagatcacga atctgcgttt tggcagcgag ctaaatacga
     5941 acaatgatgt acttaacttc gccacaactt ttccagccag aacggaaaag gtgtttgtcg
     6001 ctttcgatta tgccgctctg ccggttgggg gtgagattga cgcgttggtg tatcgcaatg
     6061 atcgcgagga tgatactcta agcagcgtgg gcgttaccaa cttgtccgga accggatcgg
     6121 cctggctgca gatcatcacg ccgttcatca acgccggtgg cctgacctcc ggccattatc
     6181 gcgtggatat ttacgtggcg ggcaagttct tggccagcgc cgagtttgat atccaataat
     6241 gcttataaca tactcagcgg atcgatatcg atccgccaag ccggacccaa ccgcagctca
     6301 cgcacgatcg aaaccggatc gggatggcgc agcgcaatct gccagcgata ttgatcgcgc
     6361 agcttggcaa agaagcacgg cgccgggccg ataatcgaat cacgcgcaat gccgatccgc
     6421 gtcagtagtg catccagttg agtcgcaact ttttcggctt cacgcttaac ccgatcggca
     6481 ttctcgtccc gaatgagcaa gcgcgccaag cgcgtgaatg gcggataagc ggtctgctgc
     6541 ctaaactcca actctttctt cgcaaaagat tcgtaatcat gctgagctgc cgcttcgatc
     6601 gcatagttgt ccggcgcata agtttgaatg atcgcccgcc cgcctaagat actgcggcct
     6661 gctcgaccga ccacttgcgc cagcacttga aacgtccgct cactcgatcg aaagtcaggc
     6721 agatgcaaag tcgtatcggc cgaaatcaca cctaccaaag tgaccaacgg cagatcgaga
     6781 cccttcgcga tcatctgtgt accgatcaag acatctgcct cgtggttaat aaagtgctgc
     6841 aaaatgattg cgtgcgaatc cttgccgcca gtcgtgtctc gatcccagcg gagtgtccgc
     6901 gcctctggca ataattcctt gag
//
LOCUS       DASRTJ010000134         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_83108,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000134 DASRTJ010000000
VERSION     DASRTJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83108"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFF70_02815"
     CDS             complement(<1..272)
                     /locus_tag="VFF70_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43656.1"
                     /translation="MSKRRPPISPLEFILIATSIGLVILVSLQLLLGSSGRVNTAGLR
                     IVQVSPTPTLTNTPRPTVTAIPPPDQVIQNTLNLPATWTPTPTWTAT"
     gene            410..604
                     /locus_tag="VFF70_02820"
     CDS             410..604
                     /locus_tag="VFF70_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43657.1"
                     /translation="MITKRQLGFAFIAIGVLIIGGVMAANLIGARDTGFGPLQKIGVA
                     LGLLIILMSIPLIRLGNKSA"
     gene            622..1278
                     /locus_tag="VFF70_02825"
     CDS             622..1278
                     /locus_tag="VFF70_02825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43658.1"
                     /translation="MNSSLTRLTKLLQIAVLAVFVLYLIIYIVYALSLFRFPYDYDQG
                     EGFELNDSILFSQGQWPYRSNEIYPFYASNYPPLFHLMVVPIFLIFGQTLLAGRVLSF
                     AATLLIAGLVGWVVHGKTRNRFIASISSLMVLASNFIYHIGPLFRQHMTMVLFELLAV
                     IFITRYADPKHSRRNIFLSMFCLLCAGYTKQLSIATVVAVLVFCFCARQENRSPPGWG
                     "
     gene            1236..2540
                     /locus_tag="VFF70_02830"
     CDS             1236..2540
                     /locus_tag="VFF70_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43659.1"
                     /translation="MRAPRKSIAAGLGLTVVAGAIFLWINVATHDQWFLNTITANANV
                     YDYQQALQLYGHFFSLHFLIAALTIGYAVYELYWDRLSAFTVWFAFSIANAAFAGKWG
                     AGESYFGTTIVAACVLSGFAVGKIKERLPQKFPKASIAIGLTIPLFYLFQAWGVRHLP
                     TDGPIFGSIADLIGVGRNTSVYFDPAGKWSYPYYDSIGYTQVGHLPTEADIRAGDLIV
                     NIIRSSGKVALTEEAMLAFRANQPVITNPTQLLNLWNNQALDPTDLIAMIDQQKFGAI
                     VFRAQFYPPPVLQAVGARYQPIDDILMNGFVYHILAPRSNYGAGCDEARFYSTIATLK
                     SIVPATGFVQIADRDSKPDGWSGPNGVSEINYLVGDRKNSQIVYRLWIGPLTWQGQPP
                     SNGSGATQLGRTNCFSFFDEVNDTNAWPNASEAVVKTLGIIH"
     gene            2731..3564
                     /locus_tag="VFF70_02835"
     CDS             2731..3564
                     /locus_tag="VFF70_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZY43660.1"
                     /translation="MLKPIDPFQIKLPDFEGPLDLLLYLIEREELDITKVSLAMVTGT
                     YLEYIHILDKLQVDQIADFLVIAAKLLLLKSQALLPRPPSIVPEGEEDVGDDLIKQLL
                     LYKQYKEVAQTLGDRETAGLHTFVRLAPPPKIVAKLDLTGISIDDLLLAVRAALEVEK
                     PAPSVGSVVKPFTLTIKDQMGMIERTLQHRPQISFKRMLADEQDRVEIIVTFLAVLEL
                     LRRRKIQVVQEQTFGDIVIVPSEEAEQSIEEAEAIEEESEFEIPKTAEEHPAEEFKFD
                     P"
     gene            3631..>3814
                     /locus_tag="VFF70_02840"
     CDS             3631..>3814
                     /locus_tag="VFF70_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43661.1"
                     /translation="MNTKYRQSFFVLTVASFMLVVVVIPWSTKLPSPAAPTSVTDIYN
                     VYYVAPSANCGIGNNPC"
BASE COUNT          913 a          980 c          928 g          993 t
ORIGIN      
        1 gttgccgtcc acgtcggcgt gggcgtccac gtcgcgggca gattgagcgt attttgaatc
       61 acttgatccg gtggcggaat tgcggtgacg gtaggacgtg gtgtattcgt tagcgtcggg
      121 gtgggcgata cttgcacgat ccgcagcccg gctgtattta cgcgcccaga tgatccgagc
      181 aaaagttgca gactcactaa gataaccaac ccaatgctgg tggcgattaa gataaattca
      241 agcgggctga tcggaggacg gcgcttggac atgcagccga ttataactca agatcgatgt
      301 gcagcgcatt tatatattcg cccttaacct tgcgaaggtt tagtaccgag tgccgatctt
      361 caaatccaac tttcgcgaag tttaagcttt caatgttata atcgcgccga tgattaccaa
      421 acggcaactc ggttttgcgt tcattgcgat cggtgtgctc atcattggcg gcgtaatggc
      481 cgccaatttg atcggggcgc gcgataccgg cttcggtccg ctccaaaaaa tcggcgttgc
      541 tttaggcttg ttgatcattc tcatgtcaat tccgttgatt cgattgggaa ataagtcagc
      601 ttgagcactg ctcaattagc aatgaattct tctctcaccc gattgaccaa actgctccaa
      661 atagccgtcc tcgccgtttt cgttttgtat ctgatcattt acatcgtcta cgcgctgtcg
      721 ttattccgtt tcccgtatga ttacgaccaa ggcgaaggct ttgaactcaa cgacagcatc
      781 ctattctcgc aaggccaatg gccgtatcga tcgaacgaaa tctatccgtt ttacgccagc
      841 aattaccccc cgctgtttca tttgatggtc gttccgatct ttttgatttt cggccaaaca
      901 ttgttggcgg gtagagtgtt gtcctttgca gccacattgc tgatcgccgg gctcgtcggt
      961 tgggtggtgc atggtaaaac gcggaacagg ttcattgcat cgatctccag tctcatggtg
     1021 ctggcttcga atttcatcta tcacattggc ccgctgttcc gtcaacacat gacgatggtg
     1081 ctgttcgaac tgctggccgt gatattcatt acgcgttacg ctgatccgaa acactctcga
     1141 cgcaacattt tcctgtccat gttttgcttg ctgtgcgcgg gctacaccaa gcaactatcg
     1201 atcgcaacgg tggtcgccgt attagtcttc tgtttttgcg cgcgccaaga aaatcgatcg
     1261 ccgccgggtt ggggttgacc gtcgtggccg gagcgatttt tttatggatc aatgtcgcca
     1321 ctcatgacca gtggttctta aatacgatca ccgccaatgc caacgtgtat gattaccagc
     1381 aagccttgca attatacggg catttcttca gcctacactt cttgatcgcc gcactcacga
     1441 ttggttacgc tgtttacgaa ctatactggg atcgattatc ggcttttaca gtttggttcg
     1501 ctttctcgat cgcaaatgcg gcttttgcag gcaagtgggg cgccggtgaa tcttattttg
     1561 ggacaaccat tgttgccgcg tgcgtgctat cgggctttgc ggttggcaaa ataaaagaga
     1621 ggttgccgca gaaattcccc aaagcatcga tcgcgatcgg attgacaatt cccctgttct
     1681 acctcttcca ggcgtggggc gtgcgccatc tgcccaccga tgggccgatc ttcggatcga
     1741 tcgccgatct gatcggagtc ggacgcaata catcggtata ttttgatccg gccggaaaat
     1801 ggtcttatcc ctattacgac tcgatcggtt atacacaagt tggccattta cccactgagg
     1861 ctgacatccg agccggcgat ctaatcgtca acatcattcg atcaagcgga aaagtcgccc
     1921 tgaccgaaga agcgatgctg gcctttcggg cgaatcagcc cgtcatcaca aatccaacgc
     1981 agttgctcaa tctgtggaac aaccaggcgc tcgatccaac tgatctgatc gcgatgatcg
     2041 atcaacaaaa attcggtgca attgttttcc gcgcacaatt ctatccgccg ccagtgctgc
     2101 aagccgtcgg cgcacgctat caaccgatcg acgatatcct aatgaacggc ttcgtctatc
     2161 acattctcgc gccacgatcg aattacggtg cgggctgcga tgaagcgcgc ttctacagta
     2221 cgatcgcaac attaaaatcg atcgtgccag caaccggctt cgtgcagatc gccgatcgag
     2281 attccaagcc cgacggttgg tctggcccga acggcgtatc cgagatcaat tatcttgtcg
     2341 gagatcgaaa aaattctcag attgtttatc gcttgtggat cggaccgctg acctggcagg
     2401 gccaaccgcc ttcaaacgga tcaggcgcaa cgcaattggg tcgtacgaat tgtttctcgt
     2461 tctttgatga agtcaatgat acgaatgcat ggccgaatgc gagcgaggcg gtcgtgaaga
     2521 cacttggtat aatccattga ccgcagaccg aagacgacag gtcgcagttg atcgttgttg
     2581 agtgatgaac tacagttgga cagcgtcgac aaaaatttgc gcgtagactc ttttcatttc
     2641 aagaaaacta ttaatatcat ccgcactatt ttgatctgcc tacgcgaatt gattgctgtt
     2701 tactaattac aactcactaa ccactgtctc atgcttaaac cgatcgatcc attccaaata
     2761 aaattaccag acttcgaagg cccgctcgat ctattgctct atctcatcga gcgcgaagaa
     2821 ctcgacatca ccaaagtttc attagcgatg gtcaccggca cttatctgga atacatccat
     2881 attctggaca agctgcaagt cgatcagatt gcagactttt tggtgatcgc ggccaagttg
     2941 ttgctgctca agagccaggc tctgctcccg cgtccgccat cgatcgtgcc tgaaggcgaa
     3001 gaagatgtgg gcgatgatct gattaagcag ctcctgttgt ataagcaata taaagaagta
     3061 gcgcagacat taggcgatcg agaaaccgcc ggattgcaca ccttcgttcg actggcaccg
     3121 ccgccaaaga ttgtagccaa attagatctc accggtatct cgatcgacga tctgctcctc
     3181 gctgtccgcg ccgcgcttga agtggaaaaa cctgcgccat cggtcggctc tgtggtcaaa
     3241 ccattcacgc tgacgatcaa agaccaaatg ggcatgatcg aacgcacgct gcaacaccgc
     3301 ccgcaaatca gtttcaagcg catgctggcc gacgaacaag atcgcgtgga gatcattgtc
     3361 acgttcctgg ccgtgctaga actacttcgg cgacgcaaga ttcaagtcgt gcaggaacaa
     3421 actttcggcg acatcgtgat cgttccgtcc gaagaagccg aacaatcaat cgaagaggcc
     3481 gaagccattg aagaggaatc ggaattcgaa attcctaaaa cggcagagga gcatccagcg
     3541 gaagagttta agtttgatcc gtagataaat cggatgttca tattctacat cgtaaaattt
     3601 tggttcaaat gcctatcaag agaagataac atgaatacaa aatacagaca atcgtttttc
     3661 gttttgactg ttgcttcgtt catgctagtt gttgttgtga ttccatggtc taccaagttg
     3721 ccctcacctg ctgcccccac atcagttaca gacatctaca atgtctacta tgtcgcgccg
     3781 agcgctaatt gcggtattgg gaataatcca tgct
//
LOCUS       DASRTJ010000135         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_234696,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000135 DASRTJ010000000
VERSION     DASRTJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234696"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFF70_02845"
     CDS             <1..1267
                     /locus_tag="VFF70_02845"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43662.1"
                     /translation="HWVAASTGLDLQSGNASDMATLNDHAIVLMTAYGERGSAYYLSA
                     ADQTWGAIEFDQPVTLTAVAWVEEANQNLKLLIGTADGRVLSLAANDLKIQSADSLSI
                     KGTAIQSVTTPDGQQIFFGGGSFGVLKSEDGGSTWQDTGFPDRDLVHSLQIILSPDYN
                     HDSSVYATAGQGLYRSRDGGQTWALQNMPAHAEFTIGSLALSPNFANDQTLIISGDYR
                     SPEMYRSSDGGETFNLLDAQIVITPTAADKLMIAPNGSYFAWLDYTGLFRSDDQGHSW
                     SRVLDRPEAVVQSLASDSHGAIFVGLLYGSVLHSIDRGQTWEPIGQRTFADHIWISAI
                     AISPDYPIDQLLLIGTDNGLYRSTDDGATWVRSDSGLPIDVNNAIAIAALSISPNFKI
                     DQTVFAATTLNGLSVSRDRGAHWSALEPR"
     gene            1329..2033
                     /locus_tag="VFF70_02850"
     CDS             1329..2033
                     /locus_tag="VFF70_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43663.1"
                     /translation="MPKKILIIEDDDSIATLTQLQLESKNYVVQIAPNGAEGLRQAFA
                     WQPDLVLLDIMMPDMDGWTVCQRLHEMSDMPVIFVTAIAQERNIVRGLQMGADDYIIK
                     PFSTKELHARIEAVLRRRHPGNEVDAPTRYANGWLTIDTEERAVTANGKPINLSPIEF
                     RLLMTLVQNEGQVLTHEFLLEQVWGSAYKQERHYLKLYVWYLRQKIEPDVNRPRYILT
                     ERGVGYRLAKAVPAKA"
     gene            complement(2237..>3765)
                     /locus_tag="VFF70_02855"
     CDS             complement(2237..>3765)
                     /locus_tag="VFF70_02855"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZY43664.1"
                     /translation="SQGSLPEAVRSHLDEMITAQPAQFTLAGDPATASLQVGYTAHAG
                     VPAVGQWVFALVAPFPTAIDDVPWSAVQSAWQGQPVTCEGCTFTGTLKLADSTLQALK
                     SILGDPAPNAIEVVPTDQLIQKLWAAQPALAIVPFDELNVRLKVLAIDGLTPLHRNLD
                     LNTYPLIVNVGLAGSIGDAQTVQKSFGRSITNRDESKMTILAMTGVTAMSRDFAASMD
                     TNGVLYPDKLIKDYFTTSDIVHISNEVSFDPDCPQQPTLNRGIFCSDPKYMDLLTDVN
                     VKIIELTGNHLNDYGWEPFSTTLGIYESLSIPYFGGGRTITEAARAVILEDHGNSIAF
                     VGCNPVGPSIDWVDGLNDGRPGSAPCSSPFPTMQTEIKQARAAGQVVIATLQYYEQPL
                     GDYSIETAKVQADDFAILADAGAMIVSGSQGHSIQGFGLRGNAFIHYGVGNLFFDQMQ
                     AVNLRQNFIDRYVIYNNQVLSAELLTTIRDEAALPRPMTPQERRDLLTLLFGVSNWGK
                     "
BASE COUNT          832 a          954 c         1073 g          906 t
ORIGIN      
        1 tcattgggtt gcggcaagca ccggactcga tctgcaaagt ggcaacgcga gcgacatggc
       61 aacgctgaac gatcacgcga ttgttttgat gacagcgtat ggtgaacgcg gctcagcgta
      121 ttatctgtcg gctgctgacc aaacgtgggg agcgattgag ttcgatcagc ccgtgacctt
      181 aacggctgtt gcctgggttg aggaagcaaa tcaaaatttg aagctgctga ttggaacggc
      241 cgatgggcga gtgttgtcct tggcggcgaa cgatctcaaa atacaatcgg cagactcact
      301 ctcgatcaaa ggcacagcga ttcagtccgt gaccacgccc gatggccaac agattttttt
      361 cggcggcgga tcgttcggcg tgttgaaatc tgaagatggt ggatcgacgt ggcaagatac
      421 cggctttccc gatcgagact tggttcattc gctgcagatc attctttcgc cggattacaa
      481 ccatgattca agtgtgtatg cgactgccgg tcaaggcttg tatcgatcgc gcgacggcgg
      541 gcagacgtgg gcgctgcaga atatgccggc gcacgccgag ttcacgatag ggtcactggc
      601 gctctcaccg aactttgcga acgatcaaac gttgattatc agtggagatt atcgatcgcc
      661 ggaaatgtat cggtcaagcg atggtggcga gacattcaat ttgctcgacg cacagatcgt
      721 gatcacgccg acggcggccg ataagttaat gatcgcgccg aatggatcgt atttcgcctg
      781 gctggactat actggtttgt tccgtagtga tgatcaggga cattcatggt cgcgggtgct
      841 ggatcgaccg gaggccgtcg tgcaatcgct cgcgtctgat tcgcacggag cgatcttcgt
      901 cgggttgtta tacggttcgg tgctgcattc gatcgatcgc ggccagactt gggaaccgat
      961 cgggcagcgg acgttcgccg atcatatttg gatcagcgcg atcgcgattt cgcctgatta
     1021 tccgatcgat cagctgctgc tgatcggaac agacaacgga ctctatcgca gcacggatga
     1081 tggagccacg tgggtccgat cggattcagg cttgccgatc gatgtgaaca acgcgatcgc
     1141 cattgcggct ttatcgatct cgccgaattt caaaatcgat cagacggtgt ttgcggccac
     1201 cactttgaac gggttgtctg tgagtcgtga tcgcggcgca cattggtcgg cgcttgagcc
     1261 gcgatgaatc ttagatgata ctttggatat tgaatggaac atggtagaat cgattgagtg
     1321 agcccagcat gcccaaaaaa atattaatca ttgaagacga tgattccatc gccacgctga
     1381 ctcagctgca gttggaatct aaaaactatg tggtccagat cgcgccgaac ggtgccgaag
     1441 ggctgcgcca ggcctttgcc tggcagccgg atttggtgct gttggatatt atgatgccgg
     1501 atatggacgg ctggacggtt tgccagcgct tacacgagat gagcgatatg ccggtgatct
     1561 ttgtgacggc gatcgcgcag gagcgcaata ttgtacgcgg gttgcagatg ggcgcggatg
     1621 attatattat caagcctttc agcaccaagg aattgcatgc gcgcatcgag gcggtgctcc
     1681 gacggcgtca cccgggcaat gaggttgatg caccgacgcg ttacgcgaat ggctggttga
     1741 cgatcgatac cgaggagcgc gcggtaacgg ctaatggcaa gccaattaat ctgtccccga
     1801 tcgaattccg cttgttgatg acgctggtgc aaaatgaagg ccaagttttg acgcacgaat
     1861 ttttattgga gcaagtgtgg ggttcggctt acaagcagga gcggcattat ttgaaattat
     1921 atgtctggta tctgcgccag aagatcgagc cggacgtcaa ccggccgcgg tatattttga
     1981 ccgagcgcgg tgttggctat cgcttggcca aagcggttcc cgcgaaagcg taacttatca
     2041 acacaacggg cgatcaacta tgatcgcccg tttttgtttt atttactcta atccgctcga
     2101 tcagattttc ttaatcccga tccccgtttt tgggccaacg gagttagatc gatcgattgg
     2161 gcctttgaga tattttgggt gcgaattaga ccggcaattc gcgatatgcg atccgcgata
     2221 agcggtatgc cattggctac tttccccaat tgctcacacc aaacaacagc gtcaacagat
     2281 cgcgccgttc ttgcggcgtc atcggtcgag gcagcgcggc ttcatcgcga atcgtcgtca
     2341 gtagttcggc gctcaagact tgattgttgt aaatcacata tcgatcgata aaattttgcc
     2401 gcaggttaac tgcttgcatc tgatcgaaga acagattgcc gacgccgtaa tggatgaagg
     2461 cgttgcctct cagcccgaaa ccttgaatgc tgtgaccttg actgccgctc acgatcattg
     2521 cacccgcatc agccagaatc gcgaagtcgt cggcttgcac tttcgccgtc tcgatgctgt
     2581 agtcgcccaa tggctgctcg taatattgca gtgtcgcgat gacaacttga cctgccgcgc
     2641 gcgcttgttt gatctccgtc tgcatggtcg ggaagggcga tgaacacggt gccgatccgg
     2701 gccggccatc attcaatcca tccacccaat caatcgacgg gccgaccgga ttgcagccga
     2761 cgaacgcaat cgagttaccg tgatcttcta gtatcaccgc gcgggctgct tcggtgatcg
     2821 ttcgaccgcc gccgaaatat ggaatgctta aactttcata aatgcctaac gtggtggaga
     2881 acggctccca gccgtagtcg ttcaaatggt tgccggtcaa ttcaatgatc ttcacgttca
     2941 cgtctgtcag caagtccata tattttggat cgctgcagaa gatgccgcga ttcaacgtcg
     3001 gctgctgcgg gcaatccgga tcgaacgaca cttcgttgct gatgtgcacg atgtcggacg
     3061 tggtaaagta atctttgatc aatttgtcgg gatacagcac accgttcgtg tccatcgatg
     3121 cggcgaaatc gcgcgacatc gcggtgacac cggtcatcgc cagaatggtc atcttcgatt
     3181 catctcgatt ggtgatcgat cgaccgaacg atttttgcac agtctgcgca tctccgatcg
     3241 atcccgccaa cccgacgttc acaatcagcg gatacgtgtt gagatcgagg ttgcgatgca
     3301 gtggcgttaa gccgtcaatt gccaacactt tcaagcggac atttaattca tcgaaaggca
     3361 caatcgctaa tgcgggttga gcagcccata atttttgtat cagctgatcg gttggcacca
     3421 cttcgattgc gttaggcgcg ggatcaccca agatcgattt cagcgcttgc aaagttgaat
     3481 cagctagttt gagtgtgccg gtgaaggtgc agccctcgca ggtaaccggc tggccttgcc
     3541 aagccgattg aactgcgctc cacggcacat catcgattgc ggtggggaac ggtgcgacga
     3601 gcgcgaacac ccattggccg accgccggca caccggcgtg tgcggtataa cccacttgca
     3661 agctggccgt ggccggatca ccggctaaag taaattgtgc cggttgcgct gtgatcattt
     3721 catcgaggtg cgatcgcacg gcttcgggca gcgagccttg tgagt
//
LOCUS       DASRTJ010000136         1861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7800,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000136 DASRTJ010000000
VERSION     DASRTJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7800"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1597
                     /locus_tag="VFF70_02860"
     CDS             296..1597
                     /locus_tag="VFF70_02860"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HZY43665.1"
                     /translation="MTTSSRLPGFYNLSIEERLRTIGQQVDLQPDELASLSGTGLDRS
                     AADHMIENVIGLYSLPIGVATNFRINQRDVLIPMVIEEPSVVAGASLAARLVREGGGF
                     ITSSDEPIMIAQMQILECANPHQARLALLAQKQRLIDFANTTDPVIIKLGGGARDIEV
                     HIIDQSPIGSMVVLHILYDCRDAMGANTVNTAAEALRPMVEEITGGRVNLRILSNLAD
                     HRKARATAMIPVEALAFDDFQGELVRDRISEAWAFAASDPYRAATHNKGIMNGVDAVV
                     IATGNDWRAIEAGAHSYAARSGKYTSLSNWGTDKDGNLVGTLEMPMAVGIVGGATKVH
                     PTARAALKLMGIQSARELAEAIVSVGLAQNLAAIRALATEGIQRGHMGLHARQLAIAA
                     GASDDQIDRIAAQMVREKQVRLDRAEALVKEIKETLRRVSN"
     gene            1643..>1861
                     /locus_tag="VFF70_02865"
     CDS             1643..>1861
                     /locus_tag="VFF70_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZY43666.1"
                     /translation="MGLMKSNKPVGIVGYGAYVPRYRLPASEVSRVWTEGTEGLPIKE
                     KSVPGLDEDVATMSIEAARNALARAEIDP"
BASE COUNT          441 a          460 c          561 g          399 t
ORIGIN      
        1 ctcaaaatgg ccggctgcac tccctgactg atgctttaac ttggacgcgt gtgcgaatct
       61 ccggccacca aacagttgaa ggcttcgcta aaattctagg gctgcgatag ccgtaaacga
      121 gcgcacctag tcttattgca ccggcgagtt gacacaatat tattgaagat ggatagcaag
      181 ggaggcttta acctcgaagc ttggtaggtt ttgagagacc cgtcggagta tacttgcgtt
      241 catatagtga gcccgcccaa acaatggtcg ggctcgttgc gcgaataatg gccttatgac
      301 gacttcatca cggttgcccg gattttataa cttgtcgatc gaggagcggc tccgaacgat
      361 cgggcagcag gttgatctcc agcccgatga gttagctagc ctatcaggta caggcttaga
      421 tcgatctgcg gcagatcaca tgatcgaaaa tgtgatcggt ttgtacagcc tcccaatcgg
      481 tgtggcgacc aattttagga tcaaccagcg cgacgtattg attccaatgg taattgaaga
      541 gccctcagtc gtggcgggcg catcattggc cgcgcgctta gtgcgcgagg gcgggggctt
      601 catcaccagc agcgatgagc cgatcatgat cgcgcagatg cagattttgg aatgcgcgaa
      661 tccgcatcaa gcccgcttgg cgttgctcgc tcagaagcag cgcttgatcg attttgcgaa
      721 cacgaccgat ccggtcatca tcaaacttgg cggcggcgcg cgcgatattg aagtgcatat
      781 catcgatcaa tcgccaatcg gatcgatggt ggtgctgcat attttatatg actgccgcga
      841 tgcgatgggc gcgaataccg tgaacactgc ggctgaagcc ttgcgaccga tggtcgaaga
      901 gatcacgggc gggcgtgtga acctgcggat tcttagcaac ttagccgatc atcgcaaggc
      961 ccgcgcgacg gctatgattc cggttgaggc attggccttt gatgattttc aaggggagtt
     1021 ggtgcgcgat cgaatcagcg aagcgtgggc gtttgccgcc agcgatccgt atcgagcggc
     1081 gacgcacaac aagggcatca tgaatggcgt tgatgcggtg gtgattgcga ccggcaatga
     1141 ttggcgagca atcgaagcgg gtgcacattc gtacgcggct cgatcaggca aatatacatc
     1201 gttatccaat tggggcacgg ataaagacgg caacctcgtc ggcacattgg aaatgccaat
     1261 ggccgtaggc attgtcggcg gcgcgacgaa agttcatcca acagccagag ccgcattgaa
     1321 gttgatgggc attcaatcgg cacgcgagtt agccgaagcg attgtgtccg tcggtctggc
     1381 acagaacctc gcggcgattc gcgcgttggc gaccgaaggt attcaacgtg gacacatggg
     1441 cttgcatgcg cgacaactcg caattgcggc gggcgcgtcg gacgatcaga tcgatcggat
     1501 tgcagcgcag atggtgagag agaaacaagt tcgcttagat cgagcggaag cgctcgtgaa
     1561 agaaatcaag gaaaccttgc gaagggtatc gaactaatcg tcatacctgt aactaaacct
     1621 tcgccaggtt gttgaggcaa caatgggttt aatgaaatca aacaaaccag tgggcattgt
     1681 cggttacggc gcgtatgtgc cgcgctatcg attacccgcc agtgaagtct cgcgcgtgtg
     1741 gacggaaggc acggaaggat tgccgattaa agagaaatcc gtgccgggtc tcgatgagga
     1801 tgtcgcgacc atgtcgatcg aagcagcccg caatgcgctg gcgcgcgccg agatcgatcc
     1861 c
//
LOCUS       DASRTJ010000137         7570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_83209,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000137 DASRTJ010000000
VERSION     DASRTJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83209"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFF70_02870"
     CDS             <1..225
                     /locus_tag="VFF70_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HZY43667.1"
                     /translation="QKAGAATINYGVFINTIITFLIVAFAMFMLIKAMNATRKPAPAA
                     APTTKDCPYCLSTVPLKATRCPNCTSALKS"
     gene            complement(341..1585)
                     /locus_tag="VFF70_02875"
     CDS             complement(341..1585)
                     /locus_tag="VFF70_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43668.1"
                     /translation="MVKKRTASDSTSEPPIKRSGGADLNADEANIGGDVTGRDKNVTT
                     IQGNVIYLENPSSEVVAQLLKVGLISTEVSRGSAAGIEGSKTTSEASPAPDQGTIQRI
                     DATLQLFKQSEAQGFAAQEFQAGEVRISQVDLLLKKAILLESEADEMMFTTIRQNKSK
                     LDQPAGSLVQVDLNNLMAGFDAGAHSAKLKEAYRLLQEANQIDPTQTEVLLHLAQLLI
                     QLTPDDPTDEQRILFRVQKLLSSPKNDDEQFQLAQATFLLATSGDQIQAEPLRQARSM
                     FEKLGRAEWVRQCDDLLAAPVQSPINSPMPVPAMQSLAVFQPIGRWQIRATDAAGSII
                     YVDIYPNGGCQGVQQAPAVGLNSPFIGQWAFIPYNQQLQFQGMVNGIYPFAFGIVIHG
                     QQGNGYYGVGTDGLGYFLTREG"
     regulatory      1732..1876
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2080..3069
                     /locus_tag="VFF70_02880"
     CDS             2080..3069
                     /locus_tag="VFF70_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZY43669.1"
                     /translation="MFRKSRSILIALAIMLGACAPAATPMVAPTNVPAVVPTSAPTVA
                     PTPAPQPVPVMLTDRLGHTVTLNSVPKRIISIAPSNTELLFVVGAGDQVVGREELSDY
                     PEAAKKIASIGSVYGKINTEAIVALKPDLILAAEINPPEQVKTLQDLGLTVYYLSNPT
                     DFEGLYANIKVVGQLTGHDIEAQKSINSTKARFDAVVTKVSVSSFMPKVYYELDATDP
                     TKPYTTGTGTFVDLLIKLAGGQNIGAALKDQYAQISAEEIVKQNPDVIVLGDAAYGVS
                     IDSIGARAGWASIAAVKNKAVYAFDDNLVSRPGPRLIDGLEQLAKLLHPDLFK"
     gene            3200..4186
                     /locus_tag="VFF70_02885"
     CDS             3200..4186
                     /locus_tag="VFF70_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZY43670.1"
                     /translation="MIIALLLSVAIGAVAINPIEAIKILASRLPFVHLTPDWSATSET
                     ILLSIRLPRTVLIALTGAALAGSGASYQGLFRNPLADPYLIGVASGAGLGAVIAMAWQ
                     WPASILGFFAVPIAAFIGALLTVAIVYKLAQVGRTTPVTTLILAGVAVGAFATALTTF
                     LMLQSTNEVRRAIAWMLGGFALGGWEPVIAILPYVSIGLIVLVALGRPLNVLQFGDDQ
                     AKQLGLNVDRVRLVIVVAASLVASAAVAFSGIIGFVGLAVPHIVRLLWGSDYRRLIPL
                     SIIGGAAGLLLADIIARSILAPEELPVGIITALIGAPFFLWLLRRAKLQAFW"
     gene            4236..5009
                     /locus_tag="VFF70_02890"
     CDS             4236..5009
                     /locus_tag="VFF70_02890"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HZY43671.1"
                     /translation="MLSINSLTVQLSSRIVLHDITLDVRPGEVLALIGPNGVGKSTLI
                     RAASGILKPLHGRISIDDQDVHQLPIDLRARSIAVVPQAVRLPEAFSVIDAVLMGRTA
                     YLGWLGRESEIDRSIAQEAMQRTNTLDLAVRRIGELSGGEQQRVLIARALTQAARVLL
                     LDEPTAHLDLKHQADVLNLVRALAHDQDYAVLIALHDLNLAAVYADRVALLSNGHLIA
                     LGTPEEVLTTERLSPVYGLRISVFTHPENGSPWVTAGNA"
     gene            5128..5691
                     /locus_tag="VFF70_02895"
     CDS             5128..5691
                     /locus_tag="VFF70_02895"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZY43672.1"
                     /translation="MPRLTKLYTRTGDEGTTMLGGGQRVTKESLRVEAYGTIDELNSQ
                     IGLALAHGLTPRLIEVLPVIQNELFHLGSNLAFREEDKTKYKLPQIDQRHIDQLEALI
                     DELNAVVGTLENFILPGGSIGAAQLHIARTVCRRAERIASALSRSESISEFVIPYLNR
                     LSDALFVMARYENKQQGGAETLWDERA"
     gene            5901..6188
                     /locus_tag="VFF70_02900"
     CDS             5901..6188
                     /locus_tag="VFF70_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZY43673.1"
                     /translation="MTTKVQAADETKAELQRRLRRIEGQVRGVQKMLDEDRQCHEILQ
                     QLTAIRSAVQGASLMFVQQYASDCLLGADSDSKAVRKVLLDDLVGLLGKVQ"
     gene            6203..6637
                     /locus_tag="VFF70_02905"
     CDS             6203..6637
                     /locus_tag="VFF70_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZY43674.1"
                     /translation="MTKPKLATVSWERQGLAFKGVGGSGFTIQFGDPAGPEMASPMEV
                     VAMASAACTAMDVIDILCKKKQAVTNFEVNVVGIRQAEHPRKFTEIDLEYVVFGHNID
                     PKAVERSIELSLTKYCSVNQSFSEAVKIKNRYRIIETEPVVA"
     gene            6639..7046
                     /locus_tag="VFF70_02910"
     CDS             6639..7046
                     /locus_tag="VFF70_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZY43675.1"
                     /translation="MESTTTVRWITGQQYVGIDSTNHSIVLSTKHENVGVKPSDVLLI
                     ALAACTAVDVVDILAKKRMPIDSLEIITTGQQDEDPPWTFRKIHLKYRLSGKELNDTA
                     VAQAIDLAEEKYCSVSATLRGVAEITTEFEIIS"
     gene            7064..7402
                     /gene="trxA"
                     /locus_tag="VFF70_02915"
     CDS             7064..7402
                     /gene="trxA"
                     /locus_tag="VFF70_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZY43676.1"
                     /translation="MAEPVHVTDAEFENKVLKSPTTVIVDFWAPWCGPCRTIAPVLEK
                     LAQEYDGKMIVAKVNTDDNPEYAMRCGVQGIPTMLFVRDGQIIDRVVGAAPYPMIKAR
                     LDKVLSTVAA"
BASE COUNT         1708 a         1915 c         2091 g         1856 t
ORIGIN      
        1 cagaaagcgg gcgccgcgac gatcaattat ggtgtcttca tcaacacgat catcactttc
       61 ttgattgtag cctttgcgat gttcatgcta atcaaagcca tgaatgctac tcggaagcct
      121 gcacccgccg ccgcgccgac gaccaaggat tgcccgtatt gtctatcgac cgtgcccctt
      181 aaagcgacgc gctgcccgaa ctgcaccagt gcgttgaaga gctgagcata ttgtggcgca
      241 atatttgatc gaagattaac cccaaaaaac aaagtgcgga tcgactggct cgatccgcac
      301 tttgtttttc attctaatgc ttagacaagg agaaggaaaa ctatccctcg cgggtcaaga
      361 agtaacccag gccatccgtg ccaaccccat agtaaccatt gccctgctgc ccatggatca
      421 cgatcccaaa ggcgaaggga tatatgccat ttaccatgcc ttgaaactgc agttgctggt
      481 tgtatgggat aaacgcccac tgcccgatga acggactgtt taagccaact gcgggggcct
      541 gctgaacacc ttggcagccg ccgttaggat aaatgtcgac gtagatgatg ctgcctgctg
      601 catcggtcgc gcgaatttgc caacggccaa tcggttgaaa cacggctaat gactgcatcg
      661 ccggtacggg catcggtgag ttgatcggac tttggacggg agcagccaat aaatcatcgc
      721 attgccgaac ccattccgcc cggccgagct tttcaaacat actccgcgct tgacgcagtg
      781 gctcggcttg aatctgatcg cccgaggtcg ccaatagaaa cgtggcctgc gctaattgaa
      841 attgctcgtc atcgttcttg ggcgagctga gcagtttttg gactcgaaac aaaatccgct
      901 gttcatccgt cggatcatca ggcgtcagct gaatgagcag ttgcgcaaga tgcaagagca
      961 cttcggtttg agtcggatca atttgattgg cctcttgcaa cagcctatag gcttctttta
     1021 atttagccga gtgggctcct gcgtcaaagc ccgccatcaa attgttcaag tccacttgaa
     1081 ccagactgcc ggccggttga tcgagcttgg atttattctg acgaatcgtg gtgaacatca
     1141 tttcatcagc ttcgctttcc aacaggatcg ctttcttgag tagaaggtct acctgtgaaa
     1201 tgcgtacttc gccagcttgg aattcttgag cggcgaaacc ctgcgcttcg gattgtttaa
     1261 agagttgtaa ggtggcatcg atgcgttgga tcgttccctg atcaggtgcc gggctggctt
     1321 ctgaggtggt tttgctgcct tcgatcccgg cagccgaacc gcgcgagacc tcagtggata
     1381 tcaagccaac tttcaacaac tgggccacta cttcagatga aggattctct agatagatca
     1441 cattgccttg aatggtggtc acatttttat cgcggcccgt cacatcgccg ccgatgttag
     1501 cttcgtcggc attcagatcc gcaccgccgc ttcgtttgat cggaggttcg ctagtggaat
     1561 cgctggcagt tcgcttcttg accatatcag actcctgcgt taagactttc aagagtatac
     1621 actaaaattt ttggtttcag catgaaactg aatttaactt attcaaactt attcaaaaga
     1681 aaattcatct tgacaacaat cgatctccgc agtatagtcg ccgcacaatt aaatactttt
     1741 ggcagtgcag gtgcttcatc tcccggatga gtgaagccca aacggtgaag ccggtgaaag
     1801 tccggcgctg tcgcgcaact gtaagttagc ctcgcgctaa caagtcaggt cgcccgcccg
     1861 cactgcgttt aaccacgaac ctcgccgaaa gggggtggaa aacactggcc ggctaaattg
     1921 tccagttact tttcaacccc ttgtcatcgc gacaaggggc tttttgtttg tggaaaaatt
     1981 ttttaacagc tggggcggct gccacgccgc cccagcgcag ggcatgggag ccctgcttgc
     2041 tcctactcgc taattaaatt tgtttaaggg gatgtttcta tgtttcgcaa atcacgatcg
     2101 atcctgatcg ctttagcgat catgctcggc gcatgcgcgc cagctgctac gccaatggtc
     2161 gcgccgacga atgtgcctgc agttgtgcca acttcggcgc cgactgttgc accaactcct
     2221 gcaccacagc cagtgccggt catgttgacc gataggcttg ggcatacggt tacgctcaac
     2281 tcagtgccca agcgcatcat ctcgatcgcg ccgtcgaaca cggagttgtt gtttgtcgtc
     2341 ggcgcgggcg atcaagttgt cgggcgcgaa gaactatcgg attaccccga agccgcaaaa
     2401 aagatcgcca gcatcggatc ggtctacggc aagatcaaca ccgaagcgat cgtcgcgctc
     2461 aagcctgatc tgatcctcgc agccgagatc aatccaccgg aacaagtgaa gactctgcaa
     2521 gatttaggtc tgaccgttta ttacttgtcg aacccgacag actttgaggg tttgtatgct
     2581 aacatcaagg tagtcggtca attgacgggt cacgatatcg aagcgcagaa gtcgatcaat
     2641 agcaccaaag cccgttttga tgcagtagtg acaaaagtgt cagtgtcttc tttcatgccg
     2701 aaggtttatt acgaactcga tgcgaccgat ccgacgaagc cgtataccac cggcaccggc
     2761 acgttcgtcg atttgctgat caaactggcg ggcggacaaa acatcggcgc ggctttgaaa
     2821 gatcaatatg cgcagatcag cgctgaagag atcgtcaaac aaaatccgga tgtgattgtg
     2881 ttgggcgacg cggcttacgg tgtatcgatc gattcgatcg gcgcgcgcgc gggctgggct
     2941 tcgatcgcgg cggtgaagaa caaagcggtg tatgcttttg atgataactt agtcagccgc
     3001 cccggcccgc gcttgatcga tggactggag cagttggcga agttgctgca tcccgatttg
     3061 ttcaaataga gttttgagaa tgagcgatag cgagatggag agaaggagag acaacgagac
     3121 aacgagacag cgcaaaaatg tcccgccgtc tcgccgtctt gccgtctcgc tatcgcctta
     3181 tctgatctcg ttcatcgcct tgatcatcgc cctgttgctc agcgtcgcga tcggcgcggt
     3241 ggcgatcaat ccgatcgagg cgatcaagat attggcgtcg cgtctgccgt tcgttcatct
     3301 cacgcccgat tggtcggcta catccgaaac gatcttgtta agtattcgtt tgccgcgcac
     3361 cgttttgatt gcactgacag gtgcggcact ggcgggcagt ggcgcatcct atcaaggctt
     3421 gtttcgcaat ccattagccg atccatattt gatcggtgta gcatccggcg cgggacttgg
     3481 tgcagtgatc gcgatggcgt ggcaatggcc ggcctcgatc ttgggatttt tcgccgtgcc
     3541 gattgcagcg ttcatcggtg cgttgttgac cgttgcgatc gtttacaaac ttgcgcaggt
     3601 cggtcgcacg acgccggtca ccactttgat tttagccggt gttgcggttg gagcatttgc
     3661 taccgcgcta acaacctttc tgatgctgca atcaaccaat gaagtgcgcc gcgcgatcgc
     3721 gtggatgctc ggtggttttg ctttgggtgg ctgggaaccg gtgattgcga tcttgccgta
     3781 tgtctcaatc ggtttgatcg tactcgtggc tttagggcga ccgttaaatg ttttgcaatt
     3841 cggcgatgat caagccaagc agctcggctt gaatgtcgat cgtgtgcgct tggtgattgt
     3901 cgtcgccgcc tcgctcgttg cctcggcggc cgtcgcgttc tccggcatca tcggctttgt
     3961 cggattagcc gtgccacaca tcgtccgatt attatgggga tcggattatc gtcgattgat
     4021 tcccttgtcg atcatcggtg gtgcggcggg cttgttgttg gcggacatca tcgcccgatc
     4081 gatcctcgcg cccgaagaat tgccggtcgg cattatcacg gctttgatcg gtgcgccgtt
     4141 ctttttgtgg ttgctgcgtc gagcgaagtt gcaggcgttc tggtagtacg taacttttct
     4201 tttgtcattg atcattcgcc atttgtcatt gttatatgct ttccattaat tcactcaccg
     4261 ttcaattaag ttcccgcatc gtcctgcacg acatcacgct cgatgtgcgc cccggcgaag
     4321 tgttggcatt gatcggacca aacggtgtcg gcaagtcgac gttgatccgt gcggccagtg
     4381 gcattttgaa accgctgcac ggtcgaatat cgatcgacga tcaagacgtg catcaactcc
     4441 cgatcgatct gcgagcccga tcgatcgccg tggtgccgca agccgtgcgt ttgcccgaag
     4501 cgttttcagt gatcgatgcc gtgttgatgg gccgcacggc ttatttgggt tggctgggcc
     4561 gcgaaagtga gatcgatcga tcgatcgcgc aggaggccat gcaacgcaca aacacgctcg
     4621 atcttgcagt gagaagaatc ggcgaactct caggtggcga acagcagcgt gtcttgatcg
     4681 cgcgagcgtt gacccaagcg gcgcgtgtct tgctgctcga tgaaccgacg gcgcatctcg
     4741 atttgaaaca tcaagctgat gtgctcaacc tcgttcgagc gttggcgcac gatcaagatt
     4801 atgcggtgct catcgcgctg cacgatctga acttggccgc cgtgtatgcc gatcgagttg
     4861 cgctgctatc caacgggcat ttgatcgcgc ttggtacgcc ggaagaagta ttgacaacag
     4921 agaggttgag cccggtttac ggcttacgca tcagcgtgtt cactcatcca gagaacggat
     4981 caccgtgggt aacggctggc aatgcataat gcttcatgca gaatgcataa ttgatcttta
     5041 ctcattactc gttactcatt attattcgcg tatcacgttt cacttatcga atgatcttca
     5101 ttgatcgtca caccactagg agaccctatg cccagactga ctaaactcta cactcgaacc
     5161 ggcgatgaag gcacgaccat gctcggcggc gggcagcgtg taacgaaaga gtcactgcgc
     5221 gtcgaggctt acggaacgat cgatgaattg aattcacaga tcggtttggc cttagcgcac
     5281 ggtttaacgc cgcgcttgat tgaagtcctg cccgttattc aaaacgagtt gttccatctt
     5341 ggctcgaatc tggcctttcg tgaagaagac aaaacgaaat ataaactccc tcaaattgat
     5401 cagcgtcaca tcgatcagct cgaagcgctg atcgatgaac tcaacgctgt ggttggcacg
     5461 ctggagaatt tcattttgcc cggtggatcg atcggggcgg cgcagctcca tatcgcgcgc
     5521 accgtctgcc gtcgcgccga acggatcgcc agcgcgttgt cgcgttcaga atcgatcagc
     5581 gaatttgtga tcccgtatct caatcgctta tccgatgctt tattcgtgat ggcgcgctat
     5641 gagaataaac agcagggcgg cgccgaaact ttatgggatg agcgggcttg acaatatagc
     5701 ttgtcattct gaggcgcgcg gtgccgaaga atctctatca ctgatcgggc gcattgccag
     5761 cggaggtata gatgcttcgc gttgctcagc atgacaatcg gtagttcgca accacacaac
     5821 gagcttgacg aagtcgaatc tggatggtat actcccctac agtatccgga agtttgatcg
     5881 actggaaagg tggagcaaag ttgacgacga aagtccaagc ggctgacgaa acgaaggctg
     5941 aactccaacg aaggctgcgg cggatcgaag gccaagtgcg cggcgtgcag aagatgctgg
     6001 acgaagatcg tcaatgccac gagattctgc agcagctcac tgcgattcga tcggccgtgc
     6061 agggcgcgag tctgatgttc gttcagcagt atgcgtccga ttgtttgctc ggcgcggaca
     6121 gcgacagtaa agctgtgcgc aaagtgttgc tcgacgatct ggttggcttg ttgggcaaag
     6181 tgcagtaagt gaaaggaatc caatgaccaa gcctaagcta gctacagtca gttgggagag
     6241 gcaaggtctt gcgtttaaag gggttggggg ttcggggttt accattcagt ttggtgatcc
     6301 ggccgggccg gagatggcta gcccgatgga agtcgttgcg atggcctcgg cggcgtgcac
     6361 cgcgatggac gtcatcgaca ttctgtgcaa gaagaaacaa gcggtgacga attttgaagt
     6421 gaatgttgtg ggcatccggc aggcagagca tcccaggaaa ttcacagaga tcgatttaga
     6481 gtacgtggtg ttcggtcaca acatcgatcc gaaggcggtt gaacgatcga tcgagttgtc
     6541 gttgaccaag tattgttcgg tcaatcaaag ttttagtgag gcggttaaga tcaagaatcg
     6601 atatcgcatc attgagactg aaccagtagt cgcttaacat ggaatcgacg accactgttc
     6661 gatggatcac cggccagcaa tatgtcggga tcgattcgac gaaccattcg atcgttttat
     6721 ccaccaagca cgagaacgtt ggcgttaagc cgtcagatgt attgttgatt gctttagcgg
     6781 cctgcaccgc agtcgatgtg gtggatattc ttgctaagaa gagaatgccg atcgattcgc
     6841 tggagattat tacgaccggc cagcaggacg aagatccgcc gtggaccttt cggaagattc
     6901 atttgaagta tcggctgagc ggcaaagagt tgaatgatac agccgtggcg caggcgatcg
     6961 atctagcgga agagaagtat tgttcagtgt cagccacact gcgtggcgta gcggaaatta
     7021 caacggaatt tgaaatcatt tcttaaaggt caggagagtt aaaatggcag aaccagttca
     7081 tgtaaccgat gcagagtttg agaataaagt gttgaagtcg ccgactacag tgattgtcga
     7141 tttctgggcg ccatggtgcg gtccgtgccg tacgattgcg ccggtgctgg aaaagttggc
     7201 gcaggaatac gacggcaaga tgatcgtcgc taaggtcaat accgacgaca atccggaata
     7261 tgccatgcgc tgcggtgtgc agggcattcc gacgatgttg ttcgtgcgcg acggccagat
     7321 catcgatcga gtcgtgggcg cggccccgta tccgatgatc aaagcccggc tcgacaaagt
     7381 cttgtcgacc gtcgccgcct aagttcgtca aacatctttg tcgatcaaaa cagacatcct
     7441 ctcgaagaat ctttcttcga gaggatgttt tgttgccgat tcatcttggc aggttattgc
     7501 tgcaacttga tcgattatgc gccgaacccg gcgtgctgcg aggaatgtgc cgccgtatcg
     7561 acaaatcaac
//
LOCUS       DASRTJ010000138         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_7916,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000138 DASRTJ010000000
VERSION     DASRTJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7916"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFF70_02920"
     CDS             <1..681
                     /locus_tag="VFF70_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZY43677.1"
                     /translation="VLVSGGTGSGKTTLLNILSSFIPDDERIVTIENAAELQLRQEHV
                     ITLESRPANIEGRGEITIRDLVINSLRMRPDRIIVGECRGAEALDMLQAMNTGHEGSM
                     TTLHANATRDALARMETMVLMAGMDLPHRAIREQISAAIDLIIQQERLRDGSRRIISI
                     SEVQGMEGDVITMSDIFTFERTGIENGKVIGRLVPTGLRPRFMEKIEIAGIRLPPNIF
                     GVGARSSR"
     gene            717..1685
                     /locus_tag="VFF70_02925"
     CDS             717..1685
                     /locus_tag="VFF70_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZY43678.1"
                     /translation="MQNIAYIIGVLGVVIVLVAIVLSLRGRGDISKRLETYTGATGDL
                     AAVSRDSKKRSTPVADTLNRAFANRRSAQGLSTQLARADLKMTVAEFVVLQILSAILF
                     GVLATLGFQTIILGLLGAVVGWFVPRWFVSNRQARRLHMFNDQLGDSLNLMVNGLRSG
                     YSVMQAMDSVSKEMPAPISVEFSRVVQEVQLGLTLEQTMGNMLRRIQSDDLDLMITAV
                     NVQREVGGNLAEILDVISHTIRERVRIKGEIRTLTAQGRYSGYVVSLLPIGLTLVLFC
                     INPKFVSRLFTSEGLAGFCGWAMVVCGLLMISSGFFAIQKIVNIEV"
     gene            1832..2770
                     /locus_tag="VFF70_02930"
     CDS             1832..2770
                     /locus_tag="VFF70_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZY43679.1"
                     /translation="MPSPVIVIAIGGVLVLILIIVRLGSRKPGESLQARLAEYSLREQ
                     PATLEEIELSLPFAERVIAPMVRQSTQFMLRFTPAKTLETTRRNLDLAGNPNNWGPSE
                     FFGIRALATGVLGVLIFVLLTITQADLPIRLLMASVFAAIGFFLPVFWLGSKIRGRKA
                     NVIKALPDALDLLTICVEAGLGFDQAVQKVSEKWDNELSRAFGRMLQEIRLGKTRREA
                     LRDMSSRIDVNDMTSFIAAVIQSEQLGVSMAKVLRIQSDQMRVRRRQRAEEKARQAPV
                     KMLFPLVFLIFPSIFIVLLGPAVLQLMSTSNPVTGG"
     gene            2818..3558
                     /locus_tag="VFF70_02935"
     CDS             2818..3558
                     /locus_tag="VFF70_02935"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZY43680.1"
                     /translation="MTSQLQSHSPTELPLTTPERSLWNNLIDALFPPHCGGCRANGSL
                     WCADCQSKLAFIHEPMCAKCGEPNTPSRLCSKCRQHSLKIEFIRSVVIFQGTVRDAIH
                     RFKYERLSSLAGPFGDLFAQYWITNNLQADWIVPVPLHSARQRDRGYNQSALLAKRLS
                     DRTYTPAIEPALKRIRITAVQMELDAAERRKNVAGAFECVDQRIKAKRVCMIDDVCTT
                     GATLDACAVALYQAGAASVFALTLARTP"
BASE COUNT          833 a          948 c         1022 g          924 t
ORIGIN      
        1 gttctagtct ccggcggcac cggctctggt aagacaactc tgctcaatat cttgtctagc
       61 ttcattcctg atgatgagcg catcgtcacg attgaaaatg cggccgaatt gcagctgcgc
      121 caagaacatg tgatcacgct agagtcgcgc ccggcgaata ttgaagggcg tggtgaaatc
      181 accattcgcg atttggtcat caattcgtta cgtatgcgtc ccgatcggat tatcgtcggc
      241 gagtgccgtg gcgcagaggc gctggacatg ctgcaagcca tgaacaccgg tcatgaaggt
      301 tcgatgacga cgttacacgc taacgcgaca cgcgatgcgc tggctcgtat ggaaacaatg
      361 gtgttgatgg ccgggatgga tttgccgcat cgcgcgatcc gcgagcagat ttcagccgcg
      421 atcgatctca tcatccagca ggaacgtctg cgcgatggct cgcgccggat catcagtatt
      481 tctgaagttc aaggcatgga aggcgatgtc ataaccatgt cggacatttt tacctttgaa
      541 cgcactggta tcgagaatgg taaagtgatc gggcgactgg tgcctaccgg cttgcggcca
      601 cgctttatgg agaagattga aattgccggt attcgcctgc cgccgaatat ttttggcgta
      661 ggggcacgct cgagtcgctg atctcagcct gcaattctca agggtggtaa tcggttatgc
      721 aaaatatagc ttatatcatt ggcgtgctgg gagtcgtgat cgttctagtg gcgattgttc
      781 tatccctacg ggggcgcgga gatatttcca agcgcctgga aacatatacc ggcgccactg
      841 gcgatctagc tgccgtttca cgagattcca agaagcgcag tacaccggtc gccgacaccc
      901 tcaatcgcgc cttcgccaat cgccgatcgg cccaagggtt gtccacacaa ttagcccggg
      961 ccgatctcaa gatgacggtg gctgaatttg tcgtcctgca gatcctctcg gcaattctgt
     1021 ttggggtgct ggccactctc ggttttcaaa cgatcatcct gggcttgttg ggcgcggtcg
     1081 tgggctggtt tgttccgcgc tggttcgtat caaatcgtca agcccgacgg ttacatatgt
     1141 tcaatgatca actgggtgat tcattgaact tgatggtcaa cggcctgcga tcgggttaca
     1201 gcgtgatgca agcgatggat tctgtatcga aggaaatgcc agcacccatc tcagttgagt
     1261 tttcgcgggt ggtacaagaa gttcaactgg gtctcacgct ggagcaaacc atgggcaaca
     1321 tgctgcgccg catccagagt gatgacttgg atctcatgat caccgccgtc aacgttcagc
     1381 gcgaggtggg cggcaacctg gcagaaattc tggatgtcat tagtcacacc atccgcgagc
     1441 gagtccgtat caaaggcgag attcgaacgc tcaccgcgca aggccggtat tcgggctatg
     1501 tagtcagctt actgccgatc ggtttaacgt tagtcctgtt ttgtatcaat ccgaaatttg
     1561 tctcgcgctt gttcactagc gagggactcg ccggtttctg cggctgggcg atggtcgtgt
     1621 gtggtctgtt gatgatcagc agcggatttt tcgcaatcca aaagatcgtc aacattgaag
     1681 tttaacgatt gcctcgggaa ttcagcgtga actcactgag catcttcgac ctgtgcagat
     1741 gcatcagagt gtaaggtaag tgggtaatta aaaacaggcc gctgttagag gaagtggcgg
     1801 aattacaaaa aatttcctcg ggagtttggc tatgccatca ccagtcatcg ttatcgccat
     1861 tgggggagtg ctggtgttga tcctgatcat tgttagatta ggatcgcgca agccgggaga
     1921 atcgctgcaa gcccgattgg ctgaatatag tctgcgcgaa caaccggcga cgcttgaaga
     1981 aattgaattg tcgctgcctt tcgccgagcg tgtcatcgcg ccgatggtgc gtcagtcgac
     2041 gcagttcatg ttgcgtttca ctcccgctaa aacgttagaa acaacccgcc gcaatctaga
     2101 tctcgctggc aatcccaaca attgggggcc gtcagaattc tttggtattc gagccttagc
     2161 cacgggcgtg ttgggagtct tgattttcgt cttactcaca atcactcaag cggacctgcc
     2221 cattcggcta ttaatggcgt ctgtgttcgc agcaatcggt ttcttcttgc cggtgttttg
     2281 gctgggcagc aagattcgcg gccgtaaagc caatgttatt aaagccttgc cagatgcttt
     2341 agacctattg acgatctgcg tcgaggccgg tctcggtttc gatcaagccg tgcagaaagt
     2401 atcagaaaaa tgggacaacg aactcagccg ggcgtttggc cggatgctac aggaaattcg
     2461 cctgggtaaa actcgccgcg aagctctgcg cgatatgtcc agccgaattg atgtgaacga
     2521 tatgaccagc tttatcgccg ccgtaattca atcggagcag ttaggtgtga gcatggcgaa
     2581 agtcctgcgc attcaatccg atcagatgcg tgtacgccgc cgccaacgcg cagaagaaaa
     2641 ggcgcgccag gctccggtga agatgctgtt cccgctggtc ttcttgatct tcccgtcaat
     2701 ttttattgtg ctgcttggac cggctgtgtt gcaattgatg tcaaccagta acccagtgac
     2761 tggaggatga tctcgcgttc gttatctcgt agttgatatc cttgtcgaaa ggacgcgatg
     2821 acttctcaac ttcagtctca ctcaccaacc gaattacctt taaccacccc agagcgcagc
     2881 ctgtggaaca acttgatcga tgcgctcttt ccaccgcact gcggcggctg tcgggccaat
     2941 ggcagtttgt ggtgtgctga ctgccaatcg aaactggctt tcattcatga gccgatgtgc
     3001 gccaaatgcg gcgaacccaa tacccccagc cgcttgtgca gcaaatgtcg tcagcattcc
     3061 ctcaaaattg aattcattcg atcggtggtc atttttcaag gcaccgtgcg tgacgcgatc
     3121 caccgcttca aatatgagcg gctgtcaagc ctggccgggc cgtttggcga tctattcgct
     3181 caatactgga tcactaacaa ccttcaagct gattggattg tgcccgtgcc tttacattca
     3241 gcgcgacagc gcgatcgagg ttacaatcaa tctgcattgt tagccaaacg tttatccgat
     3301 cgaacttaca ctccggcgat cgaaccagcg cttaaacgga ttcggatcac ggccgttcaa
     3361 atggagttag atgccgctga acgccgtaaa aatgttgcag gcgcatttga gtgtgtcgat
     3421 cagcggatca aggccaagcg tgtttgtatg attgatgatg tctgcaccac cggcgcaacg
     3481 ctcgatgctt gcgccgtggc attgtatcag gcgggcgcgg cgtccgtctt tgcgctgacg
     3541 ctggcccgca caccttagca tgaggagtgg aactatgaac atcgtcgtgc gtggtcgcaa
     3601 cttggaggtc acacctcgtc tacaagaata cgtggacaaa aaggttggca aactcgatcg
     3661 gttcttgcca accatcgatg aagcgcatat ggagttatcg atggagcaaa ctcaaagcgc
     3721 gcaaaat
//
LOCUS       DASRTJ010000139         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_45824,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000139 DASRTJ010000000
VERSION     DASRTJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45824"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /gene="nadA"
                     /locus_tag="VFF70_02940"
     CDS             complement(<1..311)
                     /gene="nadA"
                     /locus_tag="VFF70_02940"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZY43681.1"
                     /translation="MSAEEMYQQLKTKLHDVVPDVELRIKSGIADEINRLKIERNAVI
                     LGHNYMEPALYHSIPDYVGDSLELSRKAAQTDKDIIVFCGVRFMAETAKLLNPSKTVL
                     IP"
     gene            complement(316..1170)
                     /gene="nadC"
                     /locus_tag="VFF70_02945"
     CDS             complement(316..1170)
                     /gene="nadC"
                     /locus_tag="VFF70_02945"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZY43682.1"
                     /translation="MTDWRAELEPIIQRSLDEDIGDGDVTTNTTIPAKAMLSGEFVAK
                     ADGVIAGLEVARRAFQLVDARVQLIAQLKDGEFVHTGDVIARVTGPCRALLTGERVAL
                     NFFQRMSGIATLTRRFVEAVKFSRAIILDTRKTAPGLRVLDKWAVRLGGGQNHRLGLY
                     DMVLIKDNHIAAVGSITEAVKRVRSLDQLHRPIEVEVKNLTELREALTLNLDRILLDN
                     MSPDKMREAVKITHGRVPLEASGNVSIETVADIAATGVDYISVGALTHSVRALDISLE
                     LDSSQGGG"
     gene            complement(1343..2902)
                     /locus_tag="VFF70_02950"
     CDS             complement(1343..2902)
                     /locus_tag="VFF70_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HZY43683.1"
                     /translation="MEHDLPLHDLGLTTEQAQAQFDRLQHKLPALWKSIQTMTDDEQT
                     IVVVPSMSIDSDLKGAVLQAYEERFLFLLLLLRQPRARLIYVTSQAIHPSVIDYYLEL
                     LPGVISSHARKRLFLVAPLDGSQRPLSVKLLERPKLIEYIKSLILDPNRAHLVPYNTT
                     LAERDLALRLGIPMYGADPKYFPFGTKSGCRRIFEEEGVPHPLGYEDLHSQDDLINAI
                     VEMRKQKPSIVQVLTKLNEGVSGEGNALVKLENLPTPGSPNEKAAIEACLKQMSFELE
                     GVTYEHYMQKFNERGGIVEERIIGKNFKSPSVQLRVNPLGKLEILSTHDQLLGGPSGQ
                     SYLGCIFPANAAYAALITQEAAKVGERLAKEGVIGRFALDFVTVETDHGLEAYAIEIN
                     LRKGGTTHPFLTLQFLTDGVYDWKTGIFTAPSGRHKYFIATDHLASPLYKAFTPDDLF
                     DISMRHKLHFDQSRQIGVIFHMMTALAEAGQVGMTAVGESPQEAEALYKKAAEALDQE
                     AQAALEFSTKL"
     gene            3317..>3853
                     /locus_tag="VFF70_02955"
     CDS             3317..>3853
                     /locus_tag="VFF70_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZY43684.1"
                     /translation="MRIRLVAVIVLLNLLAAVATTMTLATALTPTAPFQLSLKWQRCP
                     TWYCETGWYASPAVVDVDGDGQVEALWGGYTLMSVNGSSGSIEWNRPKDSSSRLWPGI
                     VVADLDHNGSLEVVTASGNGIISVYNASGIPVTGWPVNPTGSGNEIRSLAVADIDNNS
                     DLEIVMCSTRSDNQWFVYE"
BASE COUNT          878 a         1094 c          999 g          882 t
ORIGIN      
        1 ggaatcaaaa ccgttttact gggattcaat agtttcgcag tctcggccat gaagcgcaca
       61 ccgcaaaaga cgatgatgtc cttgtcggtc tgggcggcct tgcggctgag ttcgagcgaa
      121 tcgccgacgt aatccgggat cgaatgatac agcgccggtt ccatgtaatt gtggccgagg
      181 atcaccgcat tgcgttcgat cttcaaccga ttgatctcgt cagcaatgcc ggacttgatc
      241 cgcagttcga catcgggcac gacgtcgtgc agtttcgttt tgagttgttg atacatttct
      301 tcagcagaca ttggtctaac ctccaccctg gcttgaatca agttccaaac taatatcgag
      361 ggcacggact gaatgagtca gcgcgccgac cgaaatgtaa tccacacccg tggcagcgat
      421 gtcggcgaca gtctcgattg agacattacc cgacgcttcg agcggcacgc gtccatgcgt
      481 gatcttcact gcctcacgca ttttgtcggg actcatattg tccagcagga tccgatcgag
      541 attcaatgtt aacgcctcgc gcagttcggt tagattcttt acctccactt caatcggtcg
      601 gtgcagctga tcgagcgatc gcacccgctt aaccgcttcg gtgatactcc cgaccgccgc
      661 gatatgatta tctttgatca gcaccatgtc atacaaaccc aagcgatgat tctgcccgcc
      721 gcccaagcgc accgcccact tatcgagcac gcgtaaaccg ggcgcagtct tgcgcgtgtc
      781 gagaatgatc gcccgcgaga atttcaccgc ttcaacaaaa cgccgggtca gcgtggcgat
      841 gccggacatg cgctggaaaa aattcaacgc gacacgctcg cccgtcagca atgcgcgaca
      901 cggaccggtg acacgcgcaa tcacatcgcc tgtatgcacg aactcgccat ctttaagctg
      961 ggcaatcaat tgcacacgtg catctaccaa ctgaaacgcc cgccgcgcga cttccaaccc
     1021 cgcgatcacg ccatctgctt tggccacaaa ttcgccgctc agcatcgcct ttgccgggat
     1081 ggtggtgttg gttgtcacgt cgccatcgcc gatgtcctcg tcgagtgagc gctggattat
     1141 cggttcaagt tctgctcgcc agtcagtcat cgccatatga gactcctgcc gttagtgtac
     1201 atcaaacact aacagacgtc aagccggaga gcgggggtga tcaagaagtg agcacgagcg
     1261 gggtaatttc agcatggaaa aacgaacgaa gcggctgatg gatggccgcc ccggttgggt
     1321 aatgctgccc cgctgctgga atttatagtt tcgtgctgaa ttccagcgcg gcctgcgcct
     1381 cttgatccaa agcttcagcg gcctttttgt acaacgcctc agcttcttgc ggtgattcgc
     1441 cgacggccgt catgcccacc tgccctgctt cagccaacgc cgtcatcata tggaagatca
     1501 cgccgatctg ccgcgactga tcgaagtgca gcttgtggcg catggaaata tcgaacaggt
     1561 catccggtgt gaaggcttta tataacggcg aagcgagatg atccgtcgca atgaaatatt
     1621 tgtgccgccc gctcggtgca gtgaagatgc ccgtcttcca atcataaaca ccgtcagtca
     1681 agaattgcag cgtcaagaac ggatgcgtgg tgccgccctt gcgcagatta atctcgatcg
     1741 cataagcttc caagccgtga tcagtctcca ctgtgacaaa atctaacgca aagcgtccga
     1801 tgacaccttc cttagccaag cgctcgccga ccttcgccgc ttcttgagta atcagtgcgg
     1861 catatgcagc attggctgga aagatgcagc caagataact ttgaccgctc ggtccgccta
     1921 agagctgatc gtgcgtcgaa agaatttcca acttgcccaa cggatttaca cgcagctgca
     1981 cgctgggact tttgaaattc ttacctatga tccgctcttc gacgatgccg ccgcgctcat
     2041 tgaatttctg catgtagtgc tcgtacgtca cgccttctaa ttcgaaactc atctgcttca
     2101 ggcacgcctc gatcgccgct ttttcattcg ggctgccggg cgtcggcaaa ttctccaact
     2161 tgaccagcgc attgccttcg ccggatacgc cttcattcaa cttcgtcagc acttgcacga
     2221 tcgacggttt ctgcttgcgc atctccacaa tcgcgttgat aagatcgtct tgcgaatgga
     2281 ggtcctcata gcctaaagga tgcggcacgc cctcttcttc aaatattcgt cggcagccgc
     2341 tcttcgtgcc gaacggaaaa tatttcggat cagcgccgta catcggaata ccgagccgca
     2401 gcgccagatc gcgttcggcc agtgtggtgt tatacggaac cagatgtgcg cgattcggat
     2461 cgagaatgag cgacttgata tactcgatca atttagggcg ctccagaagt ttaacgctta
     2521 acggtcgctg cgatccatcc aacggcgcga ccagaaacaa gcgcttgcgc gcgtggctgg
     2581 aaatcacacc gggcagcaat tcgaggtaat agtcgatcac gctcggatga atcgcctgtg
     2641 atgtgacata gatcaatcgg gcgcgcggct ggcgcaaaag cagcaacaag aagaggaagc
     2701 gctcttcata agcttgcagc accgccccct ttagatcgga atcgatcgac atcgaaggta
     2761 caacgacgat tgtctgttca tcgtcggtca tcgtttggat cgatttccac aacgccggca
     2821 gtttgtgttg caaccgatcg aattgagctt gcgcctgttc ggttgtgagg ccgagatcgt
     2881 gcagcggtaa atcgtgttcc attgtttctc ccatggtaag ttgttgcgcg tgatgataca
     2941 ggcacatgcg aatcttggca agatcaaaat aaaaacttcc gaagcctcgc cggttttgga
     3001 aattgattaa agcggaacag cctgcaactg atcaacccat taaacaaata accggggata
     3061 ctgatcgcaa tcccactgat caagatcgcg atcagccgcg gcttgagcac aaattgtgcc
     3121 agtccgtcga aagcgatctg aaacattcca aattgatcgt caccgtgctg ggcagcggtt
     3181 accggctgaa acccaaatga tgcctagcac cgtcgaagtc tgattgactt cgacggtttt
     3241 tatagccgat cggcctattg tcatgagcgc gtcctttcgc tacaatcaaa gcaattaact
     3301 gcttagcgag gttcgtatgc gaatcaggct cgtcgccgtc attgtcctcc tcaatctgct
     3361 ggccgccgta gcaaccacca tgacgctggc taccgcgctc accccgaccg cgccttttca
     3421 gttatcactg aaatggcagc gctgtcctac ttggtactgc gaaacgggtt ggtacgcctc
     3481 accggccgtt gtcgatgtcg atggcgacgg gcaagttgaa gcgctgtggg gcggctacac
     3541 gctgatgtcg gtcaatggca gcagcggctc gatcgaatgg aatcgcccaa aggacagcag
     3601 ttcgcgcttg tggccgggca tcgtcgttgc cgatctcgat cacaacgggt cgcttgaagt
     3661 ggtcaccgcc agcggcaacg gcatcatctc cgtttacaac gcgagcggca tccctgttac
     3721 gggctggcca gtcaacccta cgggcagcgg caatgaaatt cgcagcctgg ccgttgccga
     3781 catcgataac aatagcgatc tagaaatcgt gatgtgctcg acgcgcagtg acaatcaatg
     3841 gtttgtgtac gaa
//
LOCUS       DASRTJ010000140        14448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_45883,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000140 DASRTJ010000000
VERSION     DASRTJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45883"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VFF70_02960"
     CDS             <1..1557
                     /locus_tag="VFF70_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZY43685.1"
                     /translation="VMAGVLTDLPRSKDPQPSVTAGALQLTQSVELIDAALTIDPARS
                     GQTTFKVQLAQNGQPINNAQSVSFRFTYLTRGLGTTKAEAAPLPEGSFAASGAYLSLS
                     GLWQIEVAARRSDAPDVFAAYRVRIGADGLIAAADRSTLLDDLTHWLSIYGLIFGGVL
                     ALVLGMIWLVISFNATSKRWIQAVLLIPTVIAIPIGVSSIVTFLREATPGLTLTNPFI
                     PDEQSLSIGNKLFTQNCAACHGDAGRGNGPATADPKIRPPDFGSGHLDIHTDGDIFYW
                     IQNGFGGDSPMPLFKGKLSDDETWNLVNTVRRWRNEASGPASAATPTSSSVLQPFTPD
                     SFIAPNVVVTTTTTATPAARRNDPEALDLLGRMDRSMNALTSLTEDQDIKDDAGHELI
                     VRFDYAAPDRLRYIIQNGATSIEIGTLNYQQKPDGTWLKNDRAVPFKWPNFSYGTVAS
                     NAQIQPNEDINGVKAAVVSFDYSNFSWQVLIDPSTSRILKLTMDGSGHHMISIFSNFN
                     SAPAIDAPSP"
     gene            complement(1619..3568)
                     /locus_tag="VFF70_02965"
     CDS             complement(1619..3568)
                     /locus_tag="VFF70_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZY43686.1"
                     /translation="MPNLVKPQAGSGGLNLRSTHQIDPATLLGTLKEGVTLEVIDQSI
                     AGWYACKVFASTQVFSAAGNTVTLLPDKDAGNLRSAANTDPASVVAILKPGQQLQLIS
                     ASGDWITAKVYASADVSDLIMQAGSQPAPQPTAGSVSDRFDAPIGSDQERASAQIWPG
                     AWTDAANFATHFQARAGGPTICSTGVHLQLPNNAAALSPVFASAAGAVHFTGADPSWG
                     NVVAIEHVLADGPRVWSRYAYLDNIVVRPGDNVSRGQLIGHIGNGYSKTAYQLHFDIA
                     KIDLAQNPLDWPGHDEDRVRQNYLDPKSFIQTHRAQAAQPVVQPAVPPAPKPAPQPTT
                     GPITHALVKLKLGLHDSEGGEWLQGKQIKGVTLALVAVQEEPLFVDFTPMANAGIDVI
                     LRVGYGYADGTGTIAPPDQLAKFEKAAIDTINGAKGIAAAMYCNEINNPQEYPGFDLD
                     KGEPGPHCFHITPDYYIASYNRVFAAVKPGQKLGPAPLDPYFGPQFPWLKFTSDNREW
                     WRAILKGINGAGTFFFHGKTQSNDPAEVRSAVKFDGQPLTWQFLHFRTMETYLAEIPD
                     RFKAVPFYITEANPQRLSNGQLGWDSNNAAWITECMAYLKDWNADATHHPVSGVLFYR
                     WQNDQWSLENAGPIKKAIIAEAAKL"
     gene            3794..5062
                     /locus_tag="VFF70_02970"
     CDS             3794..5062
                     /locus_tag="VFF70_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZY43687.1"
                     /translation="MSSDPDDSMVRPKTFQCPTCGTPLQIGTDPSLQCPSCGNTVLIP
                     SKYRSQQPQQPQFVFQPMMPAFDMPNVDQMVRRGQRMGLIITIVILVFTFAIVGFSLL
                     ATNSFSQSFTADLQNTIGNITNGIPNISIPNVTIPASGKVPTAVPLAGVILEFGGKGS
                     GAGLFDDSRYIAVDKEGSIFVADYQDGHVQKFDPAGKFQLLINVPPDKNDNVIIRGLA
                     ADYQGNLYVSRGGDILKFSSADGSLVQTMAGHFPDTYYDALVVDASNTLYAIHSSAGQ
                     DDLLKLDAKGKVLARYKKIVSSVNKNDPAFNLEPVVDGVGNIYMISEIGDQVYIYDKD
                     GKFTNRFGEKGNQPGQLDHPGPAVMDGQNRLIIFNSGRLDRFSASGRYLDGQTIGYAK
                     GSPMGMAIDLDGNVYLVTNNGIVQKYQFGK"
     gene            5112..6026
                     /locus_tag="VFF70_02975"
     CDS             5112..6026
                     /locus_tag="VFF70_02975"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZY43688.1"
                     /translation="MNYHRPSLFWPIILIGAGAILLLSNLHVITGNPWGVIWRLWPLL
                     LIAIGLDILFGRRSVAGSIIGGILGLAVVGIVLWVVIAQPALPGLNFSGNLQTQHIQY
                     PLDNLQSASVSIGFATGTNELKALGDSGNLIEGDLSTYGSIDFTPTSDGSQANVRLNE
                     SSGAFGFPFLGDSGEHWAIGLNPKVVYALDLNMGVGNSTVDLGKLTLSNGKINVGVGQ
                     VEVWLPSTGKFTLKIDGGVGNLKIHMPGRVGVQVDLDSGLGSFNAGSRLRSIGDNVYE
                     TDGFSSASDQITLKIDAGVGSINIIDAE"
     gene            6137..7024
                     /gene="pdxS"
                     /locus_tag="VFF70_02980"
     CDS             6137..7024
                     /gene="pdxS"
                     /locus_tag="VFF70_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZY43689.1"
                     /translation="MQPDVGTVVIKRGLAQMLKGGVIMDVVTADQAKIAEDAGACAVM
                     ALERVPADIRAQGGVARMSDPELINKIIDAVSIPVMAKCRIGHFVEAQILEALGVDFI
                     DESEVLTPADEAHHIDKQNFTVPFVCGCRNLGEALRRIGEGAAMMRTKGEAGTGDVVE
                     AVRHARSVLGEIRRLQTMAPEELMAYAKEIGAPYDLVVETKELGRLPVVNFAAGGIAT
                     PADAALMMQLGVDGNFVGSGIFKSGDPARRAKAIVDATTHFRDAHILAEVSKNLGEPM
                     VGRTAQSLPEAERLATRGW"
     gene            7018..7500
                     /locus_tag="VFF70_02985"
     CDS             7018..7500
                     /locus_tag="VFF70_02985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43690.1"
                     /translation="MVDRLPLREANIDDIPALVQHRRWMFEDMALAENHHIDPADLDQ
                     MDKKYADDLQQHLIDGRLQAWVIEGDDRIVASGSVWFIDWLPRPDDFTGRLPYMHSVY
                     TEPEYRGVGLARRIIVAMLDECRAQGLKRISLHASPAGRSIYEALGFKSTNEMRLVFE
                     "
     gene            7517..8095
                     /gene="pdxT"
                     /locus_tag="VFF70_02990"
     CDS             7517..8095
                     /gene="pdxT"
                     /locus_tag="VFF70_02990"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZY43691.1"
                     /translation="MKIGVLALQGAFIEHEQVLRRLGAQTIEVRLPKDLTGLDGLIIP
                     GGESTTIGKLAVQYNLIDPLRALAQSGQPMWGTCAGMIFMSKDVGNDQPLLKLMDVKV
                     QRNAFGRQVDSFEADLDVKGIDDHRLFHAVFIRAPLIESVGAGVEVLATVDRAKGPAV
                     VAARQNNLVVTSFHPELSGDDRFHQLFLKMVK"
     gene            8147..9112
                     /locus_tag="VFF70_02995"
     CDS             8147..9112
                     /locus_tag="VFF70_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43692.1"
                     /translation="MIRPFDFRDVALVAQIEHQGTSLNAELALTRQPRPLQAALAGFF
                     SLNGRGLRTYILRAENDATKLTGLIQLRQRTNLKHGVIAYMAPSLQNDPRAPEAWGQL
                     LDHAAVQAGQLGIQHLVAEAPEDSEAIEILQRAGYAIYLRQDMLRLVRTRLTISADPI
                     MRLSESHDEWGIQQLYSNIAPRLVQQAEGLPKVHRTGAVRGYVLEDQMEIVAYLQIRR
                     GPDGAWFNVLIHPRVEARASQVIQFGLSLFGSNWNAPIYCGVRRYQEWLRRPLESLGF
                     EGFGSSVVMVKHLVKIVLEPELELSRAQVLVDSGAKVAAQMVDGR"
     gene            9229..10878
                     /locus_tag="VFF70_03000"
     CDS             9229..10878
                     /locus_tag="VFF70_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZY43693.1"
                     /translation="MPQQHITDDLDILLGVLPLEIAEAVRRADQNDKLLEIVMDLGRR
                     PEARYTDHELVLSEREITQADIDLVVERIGDFDADNRAGLERTLHRLSGIRNRRGHIV
                     GLTCRVGRAVYGTIDIIQDIVAAGKSLLILGRPGVGKTTMLREVARILADGYGAEQQS
                     KRVVIVDTSNEIGGDGDIPHPAVGRARRMQVMKPSLQHEVMIEAVENHNPEVIVIDEI
                     GRELEAEAARTIAERGVQLIGTAHGNTLDNLLLNPTLSDLVGGIESVTLSDEEARRRG
                     TQKTVLERRAPPTFDVLIELQDRQRLIVHHDVATSVDNLLRGRQIQTELRYRDENGVV
                     HREAAQALTIDRKQNRREREPQLMLDTRRNGDRDTELPPEFEEDQSGSNDLAMIPAMT
                     ARGSLKPIKAFGYGIARNRIEEGAARLRVPMTVVQDLDEADVIVTVKNYYRRRPKMIS
                     EAERHGTPIYVIRSSTAQQVDYFIADIFGLSEEQSDPYSLALREVQAAIQRVTGGARS
                     VDLGPQPPHIRRVQHELIREAQMISHSYGREPHRRVRIFRE"
     gene            10952..11581
                     /gene="tmk"
                     /locus_tag="VFF70_03005"
     CDS             10952..11581
                     /gene="tmk"
                     /locus_tag="VFF70_03005"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZY43694.1"
                     /translation="MFITFEGPDGSGKTTQIKLLSEALTAQGYHVLSLREPGGTPIGD
                     QIRDVVHALRNTEMSARAECLLYGAARAQLVDQVLKPQLAQGGIVLCDRYADSTLAYQ
                     AYGRRLDRSAVKQIISFATQDLQPDLTFYFDIDVEEGIQRRKMSGDDYNRMDDQTLEF
                     HRRVRAGYLEMIAAEPARWAKIDGTQTVDQIQVQVRSEVEKRLMDRRRE"
     gene            complement(11636..12094)
                     /locus_tag="VFF70_03010"
     CDS             complement(11636..12094)
                     /locus_tag="VFF70_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZY43695.1"
                     /translation="MPIYEYRCSNCKQKVSIFFRSFSAVDHDSARCPNCGSAEIKRLM
                     PRVRVIRGGSVASSDSGDNFDDSMLDDLNENDPRSLGKMMRRMADETGEDLGPEFGEV
                     INRLEKGEDPEAIEQSMPELGDMMGDAGGMGEAGGMGDAMGGMGDMGMDE"
     gene            12201..12821
                     /locus_tag="VFF70_03015"
     CDS             12201..12821
                     /locus_tag="VFF70_03015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZY43696.1"
                     /translation="MSDTDPYDLPAPALLVVISGPSGVGKDSVLNRMKELGLPFHFVV
                     TMNTRPKRPNEIEGLDYHFVSVSQFVTLIERDELLEHAVVYGDYKGIPRQQVKDAMTS
                     GQDVILRIDVQGAATIKRLVPQALLIFLTASSEAELIRRLIARQTEAPEHLQTRIAAA
                     HEEMKSMQDFDYVVINRDDELDRTVADVMSIIRAEHCRVHPRVVTL"
     gene            12818..13570
                     /locus_tag="VFF70_03020"
     CDS             12818..13570
                     /locus_tag="VFF70_03020"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZY43697.1"
                     /translation="MNDTPLMTPPAPPAPRLPLARPRWTWILLVGIILVYILEELSGG
                     SQNSRVLIRFGANYGPLVSSGEYWRLFTANFLHIGIAHIAFNGYALYILGQETEALYG
                     SPRFLVIFLLTCLSGAIASFAFTYGLSAGASTGIVGLIGTQVAFFVRNRKVFGTLSRS
                     RLTNLLFIGVINIVYGLTNPSIDNFGHLGGLIGGLILGWLLCPYYQVQPQDNGTHRIV
                     DLNSLRAEWIGVLLFCALLAAAFLGALALHPA"
     gene            13622..>14448
                     /locus_tag="VFF70_03025"
     CDS             13622..>14448
                     /locus_tag="VFF70_03025"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43698.1"
                     /translation="MFAQIIVLEYIYTEDEPRSFTYEIPIDLQNRLTIGHLVIVPFGN
                     RRLPGLVIALPETSAIKNLKSIEALLDPSPALSPAQFELAKWISETTLAPLHECIELF
                     LPPGIQGRVDTLYTMVDVELPAKLSATQSELVALLRKRGPLRSAQIDRALPRKNWQEA
                     IRSLTRRNLIEKQSILMPPSVRPKLAKYVRLIADNSIVNQARSDLSARSEVADRRWRV
                     LDYLARENEPVWISWVYAETGANLADLQALADRELIALGEEEVWRDPLADKSFVSIDP
                     "
BASE COUNT         3136 a         3832 c         4173 g         3307 t
ORIGIN      
        1 gtgatggcgg gcgtgctgac agatctgccg cgctcgaaag atccgcagcc aagcgtcacg
       61 gccggtgcat tgcaattgac gcaatccgtt gaattgatcg atgcggcctt gacgatcgat
      121 cccgcccgat cgggtcagac gactttcaaa gtgcagttag cgcagaatgg ccaaccgatc
      181 aataacgcgc agagcgtttc attccgcttc acttatttga cgcgcggcct cggcaccact
      241 aaagccgaag ccgccccgct gcccgaaggt tcgtttgctg cttccggcgc ttatctctcg
      301 ctgagcggac tgtggcagat cgaagtggcg gcgcggcgca gcgatgcgcc cgatgtgttc
      361 gcagcctatc gcgtgcgcat cggcgcggac ggtttgatcg ccgccgccga tcgatcgacg
      421 ctgctggatg acctcacgca ttggttatcg atctacggtt tgatcttcgg cggagtgctg
      481 gcgttggtgc tggggatgat ctggctcgtg attagtttca atgcgaccag caaacgctgg
      541 attcaagccg tgctgctcat cccgacggtg atcgccatcc cgatcggagt gagctccatc
      601 gtcacatttc tgcgcgaagc cacgccgggt ttgacgctga ccaatccctt tataccagac
      661 gagcagagct tatcgatcgg caataaattg tttacgcaaa actgcgcggc gtgtcacggc
      721 gatgccggac gtggcaacgg cccggccaca gctgatccta aaattcgtcc gcccgatttt
      781 ggtagcggcc atctcgacat tcacaccgac ggcgatattt tctattggat tcaaaacggt
      841 ttcggcggcg attcgccgat gccgctcttc aaaggcaaac tgtcggacga tgagacgtgg
      901 aacttggtga acaccgtgcg acgctggcgc aacgaagcga gcggaccagc ctctgcggcg
      961 acgccaactt cgtcttccgt gctgcagccc ttcacgcccg attcgttcat cgcgccgaat
     1021 gtggttgtca ccacgacgac gactgccacg cctgctgcca gacgcaacga tccggaggcg
     1081 ctcgatctgt tgggccgcat ggatcgatcg atgaacgcgc tgacctcact caccgaagat
     1141 caagatatca aggatgatgc cggccatgag ttgatcgtcc gctttgatta cgccgcgccc
     1201 gatcggttgc gctacatcat ccagaacggc gccacctcga tcgagattgg cacgctgaac
     1261 tatcaacaga aaccggatgg cacgtggctc aagaacgatc gcgcagtgcc gttcaagtgg
     1321 ccgaattttt cttatggcac tgtcgcgtcg aatgcgcaga ttcaaccgaa cgaggatatc
     1381 aatggcgtga aagctgcggt cgttagtttc gattacagca atttttcatg gcaagtcttg
     1441 atcgatccgt caacgtcacg tatcttgaag ttgacgatgg atggatcggg tcatcatatg
     1501 atctcgatct ttagcaattt caattcagcc ccggcgatcg atgcgccgag tccgtgaata
     1561 aaaatatgcg acgcactttc aaagtgcgtc gcatatgcta ctacttaaaa atctctcgtt
     1621 acaacttcgc cgcttcggcg atgatcgctt tcttgatcgg ccccgcattc tccagcgacc
     1681 actgatcgtt ctgccagcgg tagaacaata cgccgctcac cggatgatgt gtcgcatccg
     1741 cattccaatc ttttaagtac gccatacatt ccgtgatcca cgctgcatta ttcgaatccc
     1801 agccgagttg tccgttcgaa agccgttgcg gattggcttc ggtgatgtag aacggcacag
     1861 ccttgaatcg atcgggaatc tcggccagat aagtttccat tgtgcggaag tgcaggaatt
     1921 gccacgtcag cggctgcccg tcgaatttca cagccgatcg gacttcagcc ggatcgttgg
     1981 attgcgtctt gccgtggaag aaaaatgtgc ccgcgccgtt gatgcctttc aagatcgcgc
     2041 gccaccactc gcggttatca gaggtgaatt tcagccacgg aaactgcggg ccaaaatatg
     2101 gatcgagcgg tgccggcccc agtttctgac ccggtttcac ggcggcaaat acacgattgt
     2161 atgaggcgat gtagtaatcg ggcgtgatgt gaaaacaatg cgggccgggt tcgcccttat
     2221 ccagatcgaa gccgggatac tcttgcgggt tgttgatctc attacagtac atcgctgccg
     2281 caataccttt tgccccgttg atcgtatcga tggcggcttt ctcaaacttg gccagctgat
     2341 cgggcggcgc gatcgtgccg gtgccatccg catagccgta accgacgcgc agaatcacat
     2401 cgatcccggc gttggccatc ggcgtaaagt cgacaaagag aggttcctct tgtacggcga
     2461 caagcgccaa agtcacaccc ttgatctgtt tgccttgcaa ccactcgccg ccttcggaat
     2521 cgtgcaggcc gagtttcaat ttcaccagtg catgtgtgat tggcccagtg gtgggttgag
     2581 gcgcgggctt tggtgctggc ggcacagctg gttgaacgac aggttgggct gcttgcgctc
     2641 gatgtgtttg gataaatgac tttggatcaa gataattttg cctgacacga tcctcgtcat
     2701 ggccaggcca atcgagcggg ttctgcgcga gatcgatctt ggcaatgtcg aaatgaagtt
     2761 ggtacgctgt tttgctgtag ccgttaccga tatgcccgat gagctgtccg cgcgaaacgt
     2821 tatcacccgg ccgcacgacg atgttgtcaa gataggcata gcgcgaccac acgcgtgggc
     2881 catcggccaa gacgtgctcg atcgcgacga cgttgcccca agatggatcg gcgccggtga
     2941 aatgcaccgc accagctgcg gaggcaaaca ctggcgatag cgctgcggcg ttgttgggta
     3001 attgcaaatg cacgccggtg ctgcaaatcg tcggcccgcc cgctcgagct tgaaagtgtg
     3061 tggcgaaatt cgcggcatcg gtccaagcgc ccggccatat ttgtgcggac gcgcgttctt
     3121 gatcggatcc gatcggcgca tcaaaccgat cggatacaga accagccgtt ggttgcggcg
     3181 cgggttgcga tccggcctgc ataatcaaat cgctaacatc cgcactggca taaactttcg
     3241 ccgtgatcca atcgcccgag gcgctgatga gttgtaactg ttggccgggt ttcaatatgg
     3301 cgacaacact ggccggatcg gtgttggcgg ccgatcgaag gttgcccgca tctttatcgg
     3361 gcagcaacgt gacggtattg cccgcagccg aaaacacttg tgtggaggca aagactttac
     3421 aggcgtacca gccggcgatc gattgatcga ttacttccag cgtgacgccc tctttcagtg
     3481 tgccgagcaa ggtggcggga tcaatttgat gggtgctgcg cagattcaat ccacctgatc
     3541 cggcttgagg cttgactaaa ttaggcatag cggctctttt ccttggcgaa tcagaacaaa
     3601 ttttggaagc gacatttatc caatcaactt cctggtcaca atatactacg attcgaattt
     3661 gaaagtcaaa tgccggtgca gttggtgcga agctaattcg gacgccgggc ttggcgctta
     3721 aacagctgcg cctgttcaat atactcaaat cagtttacaa ttaggctagc catttccgga
     3781 gggagaattg aacatgtcca gcgatccaga tgatagcatg gtcaggccga aaacttttca
     3841 gtgtcccact tgcggcacgc ctttacagat tggcaccgac ccgtcgctcc aatgtccgtc
     3901 gtgcggcaac acggtgctca ttccatccaa gtatcggtcg cagcaaccgc agcagccgca
     3961 attcgttttt cagccgatga tgcccgcgtt cgatatgccg aatgtcgatc aaatggtgcg
     4021 gcgcggccag cgcatggggc tgatcatcac gattgtgatc ttggtgttta cttttgcgat
     4081 cgtcggtttc tcgctattag ccaccaactc gttttcacag agtttcacgg cggatcttca
     4141 aaacacgatc ggcaacatca ccaacggtat tcccaatatc agtatcccca acgtcacgat
     4201 tcccgccagc ggcaaagtgc cgaccgcagt gcccttggct ggcgtgatct tggaatttgg
     4261 cggcaagggc agcggcgcgg gcttgttcga tgactcgcgc tatatcgccg tggataaaga
     4321 aggcagtatt tttgtcgccg attatcaaga cgggcacgtg cagaagtttg atccggcggg
     4381 caagttccaa ttgttgatta acgtgccgcc ggacaaaaat gacaacgtca tcattcgcgg
     4441 tctggcggcg gactatcaag gcaacttata cgtttcgcgc ggcggggaca ttctgaaatt
     4501 cagttcagcc gacggcagcc tggtccagac catggccggg cacttccccg atacctatta
     4561 cgatgcgctg gtggtcgacg ccagtaatac gttgtacgcc atacattcct cggccggtca
     4621 ggacgatttg ctcaagctcg atgcgaaagg caaagtgctg gcgcgttaca agaaaattgt
     4681 ttccagcgtc aataagaatg atccggcctt taacttggag ccggttgtgg atggcgtggg
     4741 caatatctac atgatcagcg agattggtga tcaagtctat atctacgata aggacggcaa
     4801 gtttaccaat cgatttggcg agaaaggaaa tcaacctgga caattggatc atcccggccc
     4861 ggcggtgatg gatggccaga atcgattgat tattttcaat agtggacggc tcgatcgatt
     4921 ctcggccagt ggccgttatt tggatgggca gacaatcggt tatgccaaag gctcgccgat
     4981 gggcatggcg atcgatctcg acggcaatgt gtatctggtc accaataacg gtatcgtgca
     5041 gaagtatcaa ttcggcaaat aagtatcggg agccaaaggg cggaggtaat ctttctgcgt
     5101 aaaggagcat catgaattac catcgtccga gtttgttttg gccgatcatc ttgatcggcg
     5161 cgggagccat tctgctgctc tcgaatttgc acgtgatcac gggcaatccg tggggcgtga
     5221 tctggcggtt gtggccgctg ctgttgatcg ccattggact ggacattttg ttcgggcgtc
     5281 gctcggtggc cggatcgatc atcggcggca ttttaggctt agccgttgtg gggattgtgt
     5341 tgtgggtcgt gatcgctcaa cccgctttgc ccggcctcaa tttctccggc aatctgcaga
     5401 cgcagcatat tcagtatcca ctggacaatc tccaatcggc cagtgtctcg atcgggtttg
     5461 ccaccggcac gaatgagctc aaagccctgg gcgattccgg caatctgatc gaaggcgact
     5521 tgagcaccta cggctcgatc gatttcacgc cgacctccga tggatcgcaa gccaatgtgc
     5581 gtttgaatga atcgagcgga gcgtttggct tcccattctt gggcgattca ggtgagcatt
     5641 gggccattgg cttgaatccg aaagttgtgt acgcgcttga tctcaatatg ggtgtgggta
     5701 actcaacggt cgatttgggc aaactcacat tgagcaacgg caagatcaat gtgggtgtgg
     5761 gtcaagtcga agtatggctg ccttcaaccg ggaagttcac gctcaagatc gatggcggtg
     5821 tcggcaattt gaagattcat atgcctggcc gcgtcggggt gcaggttgat ctggacagcg
     5881 gtctgggatc gttcaatgcc ggatcgcggc tgagatcgat cggagataac gtgtacgaga
     5941 ccgatggttt ttcatcggcc agtgatcaga tcacactgaa gattgacgcg ggagtcggat
     6001 cgatcaatat catcgacgcc gaataacctc agccgcagta ttaaaaacaa aagcgtaggg
     6061 gcgagatata ctcgccccta tttttttcgt ggtagaattg aatcgcaggc aacgcaatac
     6121 tttcgggagg acgaatatgc aaccagatgt gggcacagtg gtcattaaac gcggcttggc
     6181 gcagatgttg aagggcggcg tcatcatgga tgtggtcacg gccgatcaag ccaagatcgc
     6241 ggaagatgcc ggcgcgtgcg cagtcatggc gttggagcgc gtaccggccg atattcgcgc
     6301 gcagggcggc gtggcgcgca tgagtgatcc ggaattgatc aacaagatca tcgatgcggt
     6361 ttcgatcccg gtcatggcga aatgccgcat cggacatttc gtcgaagcgc agattttgga
     6421 agcgctcggc gtcgatttca ttgacgagag cgaagtcctg acgcctgccg atgaagccca
     6481 tcacatcgac aagcaaaatt tcaccgtgcc atttgtgtgc ggctgccgca acttgggcga
     6541 agcactgcgc cgaattggtg aaggcgcggc gatgatgcgc accaaaggcg aagccgggac
     6601 tggcgatgtg gttgaagcgg tgcggcatgc ccgatcggtc ttgggtgaga tccgcagatt
     6661 gcagacgatg gcgccggaag aattgatggc gtacgctaaa gagatcggcg cgccgtatga
     6721 tctggtcgtc gagaccaaag aactcggtcg attgccggtg gtgaattttg cggcgggtgg
     6781 catcgccact ccagctgatg cagcgttgat gatgcagttg ggtgtggacg gcaacttcgt
     6841 gggatcagga atttttaaga gcggcgatcc ggcgcggcgt gccaaagcca tcgtggacgc
     6901 aacgacgcat ttccgcgatg cgcatattct ggccgaagtt agcaaaaatc tgggcgagcc
     6961 gatggtcggc cgcacggctc agtcgctgcc tgaagccgaa cgcttggcca cgcgcggatg
     7021 gtagatcggc ttccactccg cgaggccaac atcgacgaca tcccggcgct ggtgcagcat
     7081 cggcgctgga tgtttgagga tatggctttg gcggagaatc atcacattga tccggctgac
     7141 ctcgatcaga tggataagaa gtacgcggac gatttgcagc agcatctgat cgatggccgt
     7201 ttgcaggcgt gggtgatcga aggtgatgat cgaattgtcg ccagcgggtc ggtctggttt
     7261 atcgattggt taccacggcc cgacgatttt accggtcgat tgccgtatat gcacagtgtt
     7321 tacactgagc cggaatatcg aggcgttggc ttggcgcggc gcatcatcgt ggcgatgctg
     7381 gatgaatgcc gcgcgcaagg cctgaagcgt atctcactgc atgccagccc cgcgggtcga
     7441 tcgatctatg aggcgctggg ctttaagtcg acgaacgaaa tgcggctggt gtttgaataa
     7501 agggttgtaa gttagaatga aaatcggcgt actcgcctta caaggtgcat tcattgaaca
     7561 cgaacaagtt ctcaggcggc tcggtgcaca aacgatcgaa gtgcgcttgc ccaaagattt
     7621 aactggactc gacggactga tcattcccgg cggcgaatcg acgacgatcg gcaaattggc
     7681 cgtgcagtac aacttgatcg atccgctgcg cgcgctggca cagtccggcc agccgatgtg
     7741 gggcacttgc gccggcatga tcttcatgtc taaagatgtg gggaatgatc aaccactgct
     7801 gaagttgatg gatgtcaaag ttcaacgcaa tgcctttggc cggcaagttg attcattcga
     7861 agccgatctg gacgtgaaag gcattgacga tcatcgatta ttccatgcgg tcttcatccg
     7921 cgcaccgctg atcgaatccg tgggcgcggg cgtcgaagtg ctcgcgacag tcgatcgggc
     7981 taaaggacca gccgttgtcg ccgcgcggca aaacaattta gtggtgacat cgtttcaccc
     8041 ggaactatcg ggcgacgatc ggtttcacca acttttcttg aagatggtaa agtagtgatg
     8101 cgccgcatga cgcttaagcg ttatgcaatc gcagcactct agcacaatga ttcgaccatt
     8161 tgattttcgt gatgtggcgc tggtggctca aattgagcat cagggcacct cgctcaacgc
     8221 cgagctggcg cttacccgcc agccgcgtcc gctgcaagct gcattggccg gcttcttctc
     8281 cttgaacggc cgcggtctgc gcacctacat cctgcgtgct gagaacgatg ctacgaagtt
     8341 gaccggcttg attcaactgc gccaacgcac taacctcaaa catggtgtca ttgcctacat
     8401 ggcgccgtcg ctgcaaaacg atccgcgcgc accggaagcc tggggacagc tgctcgatca
     8461 cgccgcggtg caggcgggac agttggggat tcagcattta gtcgccgaag cgccggaaga
     8521 cagcgaagcg atcgaaattt tacagcgcgc cggttatgcg atttacctgc gacaagatat
     8581 gctgcggctg gtgcgtaccc gattgaccat ctcggccgat ccgatcatgc ggctgtccga
     8641 gtcacatgat gaatggggca tacagcagtt gtatagcaac attgcgccgc gcttagtgca
     8701 gcaagccgaa ggcctgccca aggttcatcg caccggagca gtccgtggtt acgtgcttga
     8761 agatcagatg gagattgtcg cgtatctgca aatccggcgc gggccggatg gcgcgtggtt
     8821 caatgtgctg attcatcccc gcgtcgaagc gcgagcctcg caagtgattc agtttggctt
     8881 gagcttgttc ggatcgaatt ggaacgcgcc gatctactgc ggtgtgaggc gctatcaaga
     8941 atggctgcgc cgtccgttgg aatcactggg ctttgaaggc tttggatcga gcgtggtgat
     9001 ggtcaagcat ttagtgaaaa tagtgcttga gcctgagctc gagcttagcc gggcgcaggt
     9061 cttagttgac agcggggcga aagtcgccgc ccagatggtg gatggcagat aggagatggc
     9121 tgatagcaga tagcgtatcg cagatcgctt ttggcatttt cacttatcac gcaatacgca
     9181 ttacgcatta ctcatcacgc attactcatt actagtcaga gaaccactat gccacaacaa
     9241 cacattactg atgacttaga tatcttgctg ggggtgctgc cgcttgaaat cgcggaggca
     9301 gtccgccgcg ccgatcaaaa cgacaagctg ctcgaaattg tcatggactt gggccgccga
     9361 cccgaagccc gctacactga tcacgagctg gtgttgagtg agcgtgaaat cacgcaggct
     9421 gacatcgatc tggtcgtcga gcgcatcggt gacttcgatg ccgacaatcg cgccggattg
     9481 gaacgcactc tgcatcgcct cagcggtatt cgcaatcggc gcggccatat cgtcggcctg
     9541 acgtgccgcg tgggccgcgc cgtatatggc acgatcgata tcattcaaga cattgtcgct
     9601 gccggcaaaa gcctgttgat tttgggacgg ccgggtgtag gcaagacgac aatgctgcgc
     9661 gaagtcgcgc ggattctggc tgatggctat ggcgctgaac agcaaagcaa gcgtgtggtg
     9721 attgtcgata cctcgaatga gatcggcggc gacggcgata ttccgcatcc agcggtgggc
     9781 cgcgcgcgcc gcatgcaagt gatgaagcca tcgctgcagc acgaagtgat gatcgaggcg
     9841 gtggagaatc acaaccccga agtgatcgtc atcgacgaga tcgggcgcga acttgaagcc
     9901 gaagcggcgc gcaccatcgc tgaacgtggc gtgcagctga tcggcacggc gcacggcaac
     9961 acactcgata atctgctgct caatccaacg ctctcggact tggtgggtgg cattgaaagc
    10021 gtcacattat ctgatgaaga agcgcgtcga cgcggcactc agaagacggt gttagagcgc
    10081 cgcgctccgc cgacgttcga tgtgctgatt gagctgcaag atcgacagcg cctgatcgtg
    10141 catcatgatg tggcgacctc ggtcgataat ttgctgcgcg gccgtcaaat tcagaccgaa
    10201 ctgcgttacc gggatgaaaa cggtgtcgtg catcgtgaag ccgcgcaagc cctaacgatc
    10261 gatcgcaagc aaaaccggcg cgagcgtgaa ccgcagctca tgctcgatac gcggcgcaat
    10321 ggcgatcgag atacagagct gcccccagag tttgaagaag atcaatcggg gtccaacgat
    10381 ctcgccatga ttccggccat gaccgcgcgc ggatcgctca agcccatcaa agcatttggc
    10441 tatggtatcg cccgcaatcg gatcgaagag ggcgcggcaa ggctgcgcgt gccaatgacc
    10501 gtggtgcagg atttggacga agccgatgtc atcgtcaccg tgaagaatta ttatcgccgc
    10561 cggccgaaga tgatcagcga agccgaacgg cacggcacgc cgatttatgt gattcgatcc
    10621 agcacggcgc agcaagtgga ttatttcatc gccgatattt ttggcttgag cgaagaacaa
    10681 agcgatccgt attcgctggc gctgcgagaa gtgcaggcgg cgatccagcg tgtgaccggc
    10741 ggcgcccgat cggttgatct cggtccgcag ccgccgcata ttcggcgcgt gcagcatgaa
    10801 ctgatccgtg aggcgcagat gatctcacat tcgtacggcc gtgagccgca tcggcgcgtg
    10861 agaattttta gagagtgatt cgatcctccc gcaggtttgt ggctgaacgc catcgttcgt
    10921 ttgaacctgc gggcggttga tttataaaac tatgttcatc acttttgaag ggccggatgg
    10981 tagcggtaag acgacacaga tcaaactatt gtctgaagcc ttgacggcgc aaggctatca
    11041 tgtgttatcc ttgcgtgaac ccggcggcac gccgatcggc gatcagattc gcgatgtcgt
    11101 gcatgcattg cgtaacacag agatgagtgc gcgggccgaa tgtttgttgt atggcgcggc
    11161 gcgtgcgcag ttagtcgatc aagtattgaa gccgcagctg gctcaaggtg ggattgtgtt
    11221 gtgcgatcga tatgccgata gcacgctggc gtatcaagct tatgggcgca ggctcgatcg
    11281 atcggcggtg aaacagatca tttccttcgc cacgcaagac ttgcagcccg atcttacttt
    11341 ctatttcgat attgatgttg aagaggggat tcagcgcagg aaaatgagcg gcgatgatta
    11401 caatcgcatg gacgatcaga cgttagagtt tcatcgccgt gtgcgcgcgg ggtatttgga
    11461 aatgatcgcg gccgaacccg ctcgctgggc gaagatcgat gggacgcaga cagtggatca
    11521 aatacaagtg caggttcgat cggaagtaga gaaaaggctg atggatcgaa ggagggaatg
    11581 agagatagag agaaattgtt tcgctccgtc tctctttcgc tctatctctc tatctttatt
    11641 catccattcc catatcgccc attccgccca tcgcatcgcc catgccaccg gcctcaccca
    11701 ttccgccggc gtcgcccatc atatcgccta attcgggcat ggactgttcg atcgcttcgg
    11761 ggtcttcgcc cttttccagc cgattgatca cttcgccgaa ttcaggaccc aggtcttcgc
    11821 cggtttcgtc ggccatgcgg cgcatcattt tgcccaacga gcgcggatca ttttcattca
    11881 gatcgtccag catcgaatcg tcgaagttat cgccgctgtc agatgacgcg actgatccgc
    11941 cgcggatcac gcggacgcgc ggcatcagcc gcttgatctc ggcgctgccg cagttgggac
    12001 agcgcgccga gtcgtgatcg actgctgaaa agctgcgaaa gaagatcgag actttctgtt
    12061 tgcagttact gcaacgatat tcgtaaatgg gcatagaaac tccgtcagaa ctaatgagtg
    12121 attagtaaca agtgcgccag caaagcggcg aaactttcac catctgcaat tatatctgag
    12181 acgtcattta ggagcaacgc ttgtctgata ctgatcccta cgaccttcca gccccggcgc
    12241 tgctcgtcgt gatctccggc ccctcgggcg tgggcaaaga tagcgtgctc aatcgcatga
    12301 aggaactggg cttgccgttc cacttcgtcg tgacgatgaa tacgcgcccc aagcggccga
    12361 atgagatcga agggcttgat tatcattttg tctcggtcag ccagttcgtt acgctgatcg
    12421 agcgcgatga attgctggaa cacgccgtgg tgtacggcga ttacaaaggc attccccgac
    12481 agcaagtgaa agatgccatg acttccggcc aggatgtcat cctgcgtatc gatgtgcagg
    12541 gcgcagcgac gatcaagcgc ttggtgccgc aggcgctttt gatctttttg acggccagca
    12601 gcgaagccga attgatccgg cggctgatcg cgcgccagac cgaagcgccg gaacatttgc
    12661 agacgcgcat cgccgccgcg cacgaagaga tgaagagcat gcaagacttt gactatgtcg
    12721 tgatcaatcg tgacgacgag ttggatcgaa cggttgcgga tgtgatgagc atcatccgcg
    12781 ccgagcactg ccgcgttcat ccgcgggtgg taacattgtg aacgatactc cactgatgac
    12841 accgccagcg ccgcctgcgc cgcgtctgcc gctcgctcga ccgcgttgga cgtggattct
    12901 tctggtcggc atcattctgg tctatatcct tgaagaactt tccggcggca gccaaaattc
    12961 acgggtcttg attcgtttcg gcgcgaatta cgggccactc gtttcgagcg gagaatactg
    13021 gcgcttattc acggcaaatt ttttgcacat cggcattgcg catatcgcct tcaatggtta
    13081 tgcgctttat atcttgggtc aggaaaccga agcgctatat ggttcgccgc gctttttggt
    13141 gatcttctta ttaacctgtt tgtctggcgc aattgcttca ttcgctttta cctatgggtt
    13201 atcggcgggc gcatcgaccg gcatcgtcgg tctcatcggt acgcaagtcg cattcttcgt
    13261 gcgcaatcgt aaagtctttg gcacgttgag tcgcagtcgt ctgaccaatc tgttgttcat
    13321 cggcgtgatc aatattgtct atggcctcac caatcccagc atcgacaact tcggacactt
    13381 gggcgggctg atcggcggat tgatcttggg ctggctgctc tgtccgtatt atcaagttca
    13441 accgcaagac aacggcaccc atcgcattgt cgatctgaat tcgttgcgcg cggaatggat
    13501 cggcgtgctg ctgttctgcg ctttattagc ggccgctttc ctcggcgcac tggcgctgca
    13561 tccggcgtaa tcgcgtatcg ccaatcgctg attcactttt cactcgtcac tcatcactct
    13621 catgttcgcc caaatcatcg tcctcgaata catctatacc gaagatgaac cgcgctcgtt
    13681 cacttatgag atcccaatcg atcttcaaaa ccgattgacg attggtcatt tggtgatcgt
    13741 gccctttggt aatcgacgtc tgcccggtct ggtcatcgcg ctccccgaaa catcggcgat
    13801 caaaaatctc aaatcgatcg aagcgctgct cgatccgagt ccggccttgt cgcccgcgca
    13861 atttgaatta gcgaaatgga tttctgaaac caccctcgca ccgctgcatg agtgcatcga
    13921 actttttctg ccgcccggca ttcaaggccg cgttgatacg ctctatacta tggttgacgt
    13981 agaacttccg gccaaacttt cggccacgca atcagaactg gttgctctct tgagaaaacg
    14041 tggtccgctg cgcagcgccc agatcgatcg cgccctgccg cgcaaaaatt ggcaggaagc
    14101 gattcgatct ttgacgcgcc gaaatttaat cgagaagcaa tcgatcttga tgccgcccag
    14161 cgtgcgaccg aagttggcta aatacgtgcg cttgatcgct gacaactcga tcgtcaatca
    14221 agcccgctcg gatctctcgg cccgatcgga agtggccgat cggcgatggc gcgtcctgga
    14281 ttatctcgcg cgtgaaaatg agccggtatg gattagctgg gtgtatgctg aaaccggcgc
    14341 gaacttggcg gacttgcaag cgttagccga tcgagaattg atcgcactgg gcgaagaaga
    14401 agtgtggcgc gatccactag ccgataaatc tttcgtgtcg atcgatcc
//
LOCUS       DASRTJ010000141         6022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_235101,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000141 DASRTJ010000000
VERSION     DASRTJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235101"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFF70_03030"
     CDS             <1..611
                     /locus_tag="VFF70_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY43699.1"
                     /translation="IDILGQQSVDPKTTDYKALMTKLAAVNDSFPAMMIYAGISDPSN
                     AALLLKYKIEYMGDNFKVKWMGLDSLQDQAFIDAAGANQAEGAYATFNRLLKAELPPD
                     GQAFLEEYARKYGPIASSLAPYGYESVSVVINAIENICSYGGDPTDRAAVRAEVMATK
                     NFSGVLGTWSFDKYGDTTLSDMTGFVVKQGEYVPVDGINANH"
     gene            717..1382
                     /gene="rpe"
                     /locus_tag="VFF70_03035"
     CDS             717..1382
                     /gene="rpe"
                     /locus_tag="VFF70_03035"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZY43700.1"
                     /translation="MSFSVKIASSILAADFARLGDEVKLAEAAGADWIHVDVMDGRFV
                     PNLTLGPIIVEAIRRSTQLPLDVHLMVVEPEKFLDTFAKAGANRLIVHAETCPHLHRT
                     LQQIRELGAKTGVAINPATPLSAVEEILPEIDQLLVMTVNPGFGGQKFIEFCIDKVAR
                     AKKLIDQSGGAAAIEVDGGIDRSTAPKVVQAGASILVAGNAIFHHPAGISAAIQELRA
                     SIE"
     gene            complement(1446..1634)
                     /gene="rpmB"
                     /locus_tag="VFF70_03040"
     CDS             complement(1446..1634)
                     /gene="rpmB"
                     /locus_tag="VFF70_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZY43701.1"
                     /translation="MAKCERCGKGPQFGHNVSFSKRHTNRMFHPNVAVRTVMVSGRAK
                     RMHLCAQCLKTIYRQPAK"
     gene            1808..2164
                     /locus_tag="VFF70_03045"
     CDS             1808..2164
                     /locus_tag="VFF70_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZY43702.1"
                     /translation="MTTDNILGRIEISPTAIASIAAQAVTQAYGVVGLSSKNAADNLI
                     GQITGDVHRGVFVRIVNNEVVIDLYVIIEYGTRIVSVAHSVQNLVKFNIEKVLGLPVA
                     SINVHVQDLRVSDSKS"
     gene            2209..3882
                     /locus_tag="VFF70_03050"
     CDS             2209..3882
                     /locus_tag="VFF70_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZY43703.1"
                     /translation="MTATSHKKSRTLLQIDGQGLKRLVEAALAWLRYHQPEINALNVF
                     PVPDGDTGTNMLLTMQSAWAEIQHSPEENVGKIAAMVAHGALMGARGNSGVILSQLWR
                     GFAHSLEGKHTLAAINVPSATREASETAYKGVIKPVEGTILTVSRAIAAGAAEAQSET
                     DDLTVILDRMVVRAKEAVVNTPNQLEILKQAGVVDSGGQGLTVIIEGMLRYLRGESVE
                     IDQALEAAVDLKVPANTEVDAGDVAQGYSYDVQCLIKGHNMNIEEARQQINQMGLSTL
                     VVGDQETIKVHVHVPRPSMVLAYAETIGRLHDVVVEDMAAQYQQFLLGRSSHPIQTPS
                     YNLKPGEIATIAVVPGEGLTRVFQSLGITAIVPGGQTMNPSTQDFAQLIEALPTDRVV
                     ILPNNSNIILAAKQAADLVKKQIKVVPTKTIPQGVAALLAFNDQRDLESNAKSMEAAA
                     KRVQTGEVTTATRSVEFNGVKVVEGQIIGLLNDVLTAAGTSIEEVTWTLLEQMKVAGL
                     EIVTLYYGNGLLSAVADTLGAAIRGKYNHLEVEVVEGGQPHYDYIISAE"
     gene            3995..4858
                     /locus_tag="VFF70_03055"
     CDS             3995..4858
                     /locus_tag="VFF70_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY43704.1"
                     /translation="MTKVHIITDSTAHFTDPTLPERLGVTVVPQTIQFGRQTFREGVD
                     ISTEEFYRRLPHYATLPTMHPPSVDVFRDLYSEKLKQNKQIISIHTSSKMGRTVAMAR
                     QAADEFLGGNKIAVIDSLTTSLGLGLLVEAAAGAAENGATLDEVVRVVRGMITHMYAV
                     FFVDSLDYLERNGRLSKSQAVLGTMLGIKPFLTIEEGEIIPMEKVRTREKAIDKLVEF
                     VSEFSNIDRVAIMQSGQQPGEDTRLLLERLEQTFPGQYFPVMTYGPGLACQIGPDSLG
                     IFIFEGSGREN"
     gene            4918..5745
                     /locus_tag="VFF70_03060"
     CDS             4918..5745
                     /locus_tag="VFF70_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY43705.1"
                     /translation="MIHIVTDSTADIPADLCRELDIAVVPAHIIFGMETFEDGITLTR
                     DEFYHRLADASQLPTTSAPSPGEFSEVYQRLGGKIVSIHLASRLSAIYSAAYGASEMT
                     ANVSVTVFDTTQLSMGIGWQVIAAARAAKQGQSADQIIKLLEDIRPRIRLFAVLDTLE
                     YLQRSGRVGWLRGFLGQMLHIKPVVQVADGEVNLIDRIRTRHKSIERLKELVLSLGPI
                     ESLAVLHTHAHADAQTLADDFAKLFPALRESILISEATTTIGSYAGSNALGVAVVVA"
     gene            5836..>6022
                     /locus_tag="VFF70_03065"
     CDS             5836..>6022
                     /locus_tag="VFF70_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43706.1"
                     /translation="MSAKNPAFEKLAKILKLEVDTGYRDRAMVGGLSNFAARWKQEAG
                     NDAISQQVAEQLSSYGQI"
BASE COUNT         1453 a         1611 c         1641 g         1317 t
ORIGIN      
        1 ggattgacat cctcggtcaa caatctgtcg atcccaagac gaccgattac aaggcgctga
       61 tgaccaagct cgcagcagtg aatgacagtt ttccagctat gatgatttat gccggcatat
      121 cagacccaag caacgccgct ctcctcttga aatataaaat cgaatacatg ggcgacaatt
      181 ttaaagtcaa atggatgggg ctggacagtc tccaggatca agccttcatt gatgcggcag
      241 gcgccaacca ggctgagggc gcttatgcaa ctttcaatcg gctgctcaag gccgaactcc
      301 cccccgacgg ccaagctttc cttgaagagt atgccagaaa atatggccca atcgccagct
      361 cgcttgcgcc ttatggctac gaatctgtca gcgttgtgat caatgcgatc gagaacatct
      421 gcagctatgg tggtgatcct accgatcggg cagccgttcg cgctgaagtc atggcgacga
      481 agaacttcag cggagtgctg gggacgtggt ccttcgataa atatggtgac accacgttgt
      541 ccgacatgac cggttttgtg gttaaacaag gcgaatatgt gcctgttgat ggcataaacg
      601 ccaatcacta atttctcatc acgccggtga aacactgggg cggccattca acgcttgaat
      661 tgaagcctga cgcccacacc ttgatttttt accctcgaac gtgtatcata gccacgatgt
      721 ctttttctgt gaaaatagcc tcttcgatct tagcggctga ttttgcgcgc ttgggcgatg
      781 aagtgaaact ggccgaagcc gccggtgccg attggatcca cgtcgatgtg atggatggcc
      841 gcttcgtgcc gaacctcacg ctggggccga ttatcgtcga agcgatccgg cgcagcacgc
      901 aacttccact cgacgtgcat ttgatggtcg tcgaaccgga aaaatttctc gacacatttg
      961 caaaagcagg cgcgaatcga ttgattgtgc atgcggaaac ttgtccgcat ttgcatcgca
     1021 cgttacagca gattcgcgaa ctaggcgcaa agacgggcgt tgcgattaat ccggccacgc
     1081 cattgagtgc cgtcgaagaa attctgccgg agatcgatca actgctggtg atgacggtga
     1141 atcccggctt cggcggacag aagtttattg agttctgcat cgataaggtg gcacgggcaa
     1201 agaagctgat cgatcaatcg ggcggagccg cagcgatcga ggttgacggc ggaatcgatc
     1261 gatcgaccgc gcctaaagtg gtgcaggccg gcgcatcgat cttggtggcg ggcaatgcta
     1321 tcttccatca tcccgctgga atttcagcgg ccattcaaga acttcgagca tcgatcgagt
     1381 aacacgcgca aataaaaacg ccgaccctaa tgaggtcggc gttcgtcgga gaaactgggt
     1441 tacaattact tggcaggctg gcgatagatg gtcttcaagc attgcgcgca caaatgcatg
     1501 cgcttggcgc ggccactcac catcaccgtt cgcaccgcga cattcgggtg gaacatgcga
     1561 ttggtgtggc gcttcgagaa acttacattg tgaccaaatt gaggaccttt gccgcagcgt
     1621 tcacactttg ccatctcatt cacctctatg caaaataatg cgcgtctcgc gacgcgccag
     1681 atattcaatg gcggcaatct taccatatcg atttgaattg gtcaacttac ccggcaacct
     1741 cccgcaggtt tatggacgaa atgagattat tgtttttcaa cctgcgggtg gaaagagtgg
     1801 agttcatatg acaaccgata acattttagg acgtatcgaa atatcgccca cggcgatcgc
     1861 ctccatcgcg gcgcaagccg tgacgcaggc ctacggcgtg gtggggctgt ccagcaaaaa
     1921 cgcggcggac aacttgattg ggcagatcac cggcgatgtg caccgcggtg tgtttgtgcg
     1981 gatagtcaac aacgaagtgg tgattgatct atacgtcatc atcgaatatg gcacacgcat
     2041 cgttagcgtg gcgcacagcg tgcagaatct cgtaaagttc aacatcgaga aagtgctggg
     2101 gctgccagtc gcctcgatca acgtccatgt gcaagaccta cgcgtcagcg attcaaaatc
     2161 gtagtgcaag ttggtaactt gctctgcatt cctgggagta actgatcttt gacggccacc
     2221 tcgcacaaga aatctcgcac actactgcaa atcgatgggc aaggcttgaa gcgcctggtc
     2281 gaagcggcct tggcctggct gcgttatcat caaccggaaa ttaatgcgct caacgttttt
     2341 ccagtgcccg atggggacac cggcaccaac atgctgctga ccatgcagag cgcgtgggca
     2401 gaaattcagc attcgccgga agagaacgtc ggcaagatcg cggcgatggt ggcgcatggc
     2461 gcattgatgg gcgcgcgcgg caattcgggt gtgatcttgt cgcagttgtg gcgcggcttc
     2521 gcgcattccc tcgaaggcaa acacacgctg gccgcaatca atgtgccaag tgcaactcgt
     2581 gaggcaagtg agactgcgta caaaggcgtg atcaaaccgg tcgaaggcac gatcttaacg
     2641 gtatcgcgcg cgattgccgc aggcgcagcc gaagcgcaaa gtgaaaccga cgatctcacg
     2701 gttattctcg atcgaatggt ggtgcgagcc aaagaagccg tggtgaacac accgaaccaa
     2761 ttggaaatct taaaacaagc cggtgtcgtc gactccggcg gccaaggttt gacggtcatc
     2821 atcgaaggca tgctgcgcta cctgcgaggc gaatcggtgg agatcgatca ggcgctggaa
     2881 gcggcggtcg atctcaaagt gcctgccaat accgaagtgg atgccggcga tgtggcgcaa
     2941 ggttacagtt acgacgtgca gtgcttgatc aagggccaca acatgaacat cgaggaagcc
     3001 cgccagcaga tcaaccaaat gggcttatcg acgttagtcg tcggcgatca agagacgatc
     3061 aaagttcacg tgcatgtgcc acggccgagt atggtgttgg cctacgccga aacgatcggg
     3121 cggctgcatg atgtcgtcgt cgaagatatg gcggcacagt atcaacaatt tttgctgggt
     3181 cgatcatcgc atcccattca gacacccagt tacaatctaa agcccggtga gattgccacg
     3241 atcgcggtgg tgccgggcga aggtctgacg cgcgtctttc agagcttggg catcacggcg
     3301 atcgtgcccg gcggccaaac gatgaatccc agcacgcaag attttgcaca gctcattgaa
     3361 gcactgccca ccgatcgggt tgtgatcttg ccgaataaca gcaacatcat tctagccgcc
     3421 aaacaagcag cagatctggt caagaaacaa atcaaagtcg tccccacgaa aaccattcca
     3481 caaggcgtcg cggccttgtt ggcctttaac gatcagcgcg atctggagtc gaacgccaag
     3541 agcatggagg ccgcagccaa gcgcgtgcag accggcgaag tgacgaccgc cacccgatcg
     3601 gttgagttca acggcgtcaa agttgttgag ggacaaatca tcggcctatt gaatgacgtg
     3661 ctgaccgctg ccggaacatc gatcgaagaa gtgacgtgga cattgctgga acagatgaaa
     3721 gtcgctggct tggaaatcgt gacgttgtac tacggcaacg gcctgttgtc cgccgtggct
     3781 gatacacttg gtgctgccat tcgcggcaag tacaatcatc ttgaagtcga agtcgtcgaa
     3841 ggcggacagc cgcattacga ctacatcatc agcgcagagt aaggacttgg ggatcagggg
     3901 gtcagcaaga agttattacg cgatacgcat tactccttac ttattactcg ttattcacaa
     3961 aatctcactt gtcgcttttt actcttcact catcatgacc aaagttcaca tcattaccga
     4021 tagcacagca cactttaccg atcccacgtt acccgaacgc ttaggcgtca ccgtagtgcc
     4081 gcagaccatt caatttggac gacaaacatt tcgcgaaggt gtggacatca gcaccgaaga
     4141 gttttaccgg cgactgccgc attacgccac actgccgacg atgcatccgc catcggtgga
     4201 tgtgttccgc gatctgtatt ctgaaaaatt gaaacagaat aagcagatca tttcgatcca
     4261 cacttccagc aaaatgggcc gcactgtggc aatggcgcgt caggctgccg atgaattttt
     4321 gggcggcaac aagatcgcgg tgatcgattc gctgacgact tcgctcggct tgggactgtt
     4381 ggttgaggcc gccgccgggg ccgctgagaa cggcgccaca ctcgatgaag tcgtccgcgt
     4441 cgtgcgcggc atgattacgc atatgtacgc cgtgttcttt gtcgacagtc tggattactt
     4501 ggaacgcaat ggccgtctca gcaaatcgca agcggtgctc ggcacgatgc ttggcatcaa
     4561 acccttcctg acgatcgagg aaggcgagat cattcccatg gagaaagtcc gcacgcgcga
     4621 aaaagcgatc gacaagctag ttgagtttgt atccgagttc tccaatatcg atcgtgtggc
     4681 gatcatgcag agcggccagc agcccggcga agatacacgc cttctgctcg aacggttgga
     4741 gcaaactttc cccggccaat attttccggt catgacttac ggacccgggc ttgcttgtca
     4801 gatcggcccg gacagtttgg gcatttttat ttttgaagga tcgggtagag aaaactgatt
     4861 agtgatactt actgagcgat tcattcttca ccccttacta atcactcatc actaattatg
     4921 atccacatcg tcaccgatag caccgccgac attcctgccg atctctgccg cgaattggac
     4981 atcgccgtcg tgcccgcgca catcatcttt ggaatggaga ccttcgaaga tggcatcact
     5041 ctcacgcgcg acgagttcta tcaccgcctg gcagacgcgt cgcaactgcc caccacctca
     5101 gcgcccagtc ccggcgaatt ttcagaagtc tatcaacgcc tcggcggcaa gatcgtatcg
     5161 attcatctcg cttcgcgctt gagtgcgatc tactccgcag cttacggcgc gtccgaaatg
     5221 acggctaacg ttagcgtgac cgtcttcgat acgacgcaac tctcgatggg cattggctgg
     5281 caggttatcg ccgccgcccg cgcggccaaa caaggccagt ccgccgatca gattataaaa
     5341 ctgctggaag acattcgacc gcgcatccgc ttgttcgctg tgcttgatac gctcgaatac
     5401 ttacagcgta gcgggcgcgt cggctggctc agaggctttc tcggccagat gctccacatc
     5461 aaaccggtgg tgcaagtggc agacggcgaa gtcaatttga tcgatcgcat acgcacgcgc
     5521 cacaaaagca ttgaacgctt aaaagagttg gtgttgtcgc tcggcccgat cgaatcactg
     5581 gccgtcttgc acacacacgc acatgccgac gcgcagactt tagcggatga ttttgccaag
     5641 ctctttcccg ctctgcgtga atcgatcttg atcagtgaag ccactacgac gatcggctca
     5701 tacgccggat cgaacgcgct gggcgtcgcg gtcgtcgtcg cttaaggcgc agggcttaat
     5761 gtataattgc ccggatgatg ttatgatgca ctctgcatac cgcagtgtgc atttttaatt
     5821 cagaaagaat agatcatgtc ggcaaaaaat ccggcctttg aaaaacttgc caaaatcttg
     5881 aaactcgaag tcgataccgg ctaccgcgat cgggcgatgg tcggcggcct gtcgaatttc
     5941 gcggcgcgct ggaagcaaga agccggcaat gatgcaatat cgcagcaagt ggcggaacag
     6001 ttgagcagct acggtcaaat ag
//
LOCUS       DASRTJ010000142         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_83510,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000142 DASRTJ010000000
VERSION     DASRTJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83510"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFF70_03070"
     CDS             complement(<1..341)
                     /locus_tag="VFF70_03070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZY43707.1"
                     /translation="MTTTQFFFILFAIITLVSGLRVVTTRTMFHAALYLVISLFGVAL
                     LFVLLEAGFLAAVQVIVYIGAIAILIIFAVMLTRGMMESKTPMLNSQAGLAAVVAAAL
                     LFILIRVLTSFS"
     gene            complement(424..>1641)
                     /gene="nuoH"
                     /locus_tag="VFF70_03075"
     CDS             complement(424..>1641)
                     /gene="nuoH"
                     /locus_tag="VFF70_03075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZY43708.1"
                     /translation="IIANFLRSLLGAGGLHLADWFVELIMAVLRAALLGTFALLTFMV
                     LTWIERKVVARIQDRLGPNVAGPWGILLPIADGIKMLVKEDTVPAGADKVVFSLAPLV
                     AAVAAMLVYAVIPFGPDTGSGALAGADLNIGIFYILAVGAVSLIAILMAGWSSNNKYA
                     LLGAFRAVAELLSYEVPQVLSITVVVMLAGTMQMTQIVQAQTVPFIFPLFATAIIFFI
                     SGVAESGRTPFDLVEAESEIVAGYHIEYSAMKFSMFFLGEFVHAFAVCAVFATVFLNG
                     WEGPILPPYVWFLLKSGAIFFIMMWIRGTLPRFRIDQLMAFNWKFLVPLSLVNLMVVA
                     IADTIVRTTLHITPDVNGNPWAWGLILFAVNLVMLFIAFGLLGRGARRGIDDRRTITR
                     MPEDKAAAPAQAN"
BASE COUNT          432 a          494 c          421 g          294 t
ORIGIN      
        1 gagaaactgg tcaacactcg gatcaagatg aacagcaatg ccgcggccac gactgcggcc
       61 aacccggctt gcgagttgag catcggggtc ttcgactcca tcattccgcg cgtcaacatc
      121 actgcgaaga tgatcaagat cgcaatcgcg ccgatgtaga cgatcacctg caccgctgcc
      181 aggaagcccg cttcgagcag cacgaatagc agcgccacgc caaataaact gatcaccaga
      241 tacagcgcgg catggaacat cgttctggtc gtcaccactc gtaagccgga gaccagcgtg
      301 atgattgcaa ataggatgaa aaagaattga gtcgttgtca tggcttatcg cctacattag
      361 tccccgccgc gaagcggaga gggatcgctt attgcagaga gcatatcgct attgaatgtt
      421 ctcttagttc gcctgcgccg gtgcagcggc tttgtcttcc ggcatgcgtg tgatcgtgcg
      481 tcgatcatcg atgccccgcc gtgcgccgcg ccctagcaag ccaaaggcaa tgaacagcat
      541 caccaagttg actgcgaaca agatcaagcc ccacgcccat ggattgccat ttacatcagg
      601 tgtgatgtgc agcgtcgtgc gcacgatcgt gtctgcgata gccaccacca tcagattgac
      661 cagcgacagt ggcaccagga atttccagtt aaaggccatc agctgatcga tgcggaagcg
      721 tggcagcgtg ccgcggatcc acatcataat gaagaagatc gcgcccgact tgagcaggaa
      781 ccacacgtag ggcggcagga tcggaccttc ccaaccattc aaaaaaactg ttgcaaacac
      841 cgcgcacacc gcaaacgcat gcacgaattc gcccaggaag aacatcgaga atttcatcgc
      901 cgaatactcg atgtgataac cggccacaat ttccgactca gcttcgacca gatcgaacgg
      961 cgtgcgtcct gactcggcga cgccactgat gaaaaagatg atcgcggtgg caaacagcgg
     1021 gaagataaac ggcaccgtct gcgcctgaac gatctgcgtc atctgcatgg tgcctgccag
     1081 catgaccacc acggtgatgc tcaacacttg cggcacctca taagaaagaa gttcagccac
     1141 ggcgcgaaat gcaccgagta aggcatactt attgttgctg ctccaacccg ccatcaaaat
     1201 cgctatcagc gacaccgcgc cgaccgcgag aatgtagaaa ataccaatgt tcagatcagc
     1261 gccggccagt gcgccactgc ctgtatcggg accaaatgga atcacggcat acactagcat
     1321 cgccgccacg gcggcgacca gcggcgccag gctaaacacc actttatccg cacctgccgg
     1381 cacggtatct tcttttacca gcattttgat gccatccgcg atcgggagca gaatacccca
     1441 cggaccggcc acgtttggac ccaatcgatc ttgaatgcgg gccaccactt tgcgttcgat
     1501 ccaggtcaag accatgaagg tgagcagggc aaacgtgccc agcaaagccg cccgtaacac
     1561 ggccataatc agctcgacaa accaatcggc taaatgcaaa ccgcccgcgc ccaacaggct
     1621 acgcaggaaa ttggcaatga t
//
LOCUS       DASRTJ010000143         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_192749,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000143 DASRTJ010000000
VERSION     DASRTJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192749"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFF70_03080"
     CDS             <1..425
                     /locus_tag="VFF70_03080"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZY43709.1"
                     /translation="PIYHSDTPDKVARYMVEPYVIAADVYNAPSHVGRGGWTWYTGSS
                     GWMYRVGLEAILGIRRMGKALQIDPCIPKTWPSYQVTYRVGETTFQISVENPAGVNRG
                     VKQLKLDGSVLNSNEIPLLDDGSQHAVHVLLGTVTATS"
     gene            539..922
                     /locus_tag="VFF70_03085"
     CDS             539..922
                     /locus_tag="VFF70_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43710.1"
                     /translation="MKSEIGLWIDHRQAVIVVITAAGEETKQIVSNMEKHVRFSSGSS
                     EDGSQEDVRDRQFENRLNSYYDEVVAVIRHADAIQIFGPGEAKVELEKRLEHEGLKGH
                     IVGVETTDKMTDHQIAAKVRERFPA"
     gene            1042..1944
                     /locus_tag="VFF70_03090"
     CDS             1042..1944
                     /locus_tag="VFF70_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY43711.1"
                     /translation="MTKLNVAGKVIIVTGASDGIGRELALQLGARGAIVVLTARREHV
                     LEEVATRIRAAGGQGLVVPADLCDANLIAHLVERTVQEFGRIDVLINVAGMGFYDWIE
                     EMSSDELQRQYQTNVIGLADLIRQVVPIMKQQRSGHIINFSSYASRIAVPPLTIYAST
                     KYAIEGLSDALRRELAPWGIRVTRVHPWAVDTKFNEKAARHSGIIFPDERGAPVVAIT
                     EVTRETVAKRVIAAIERPRRAVYVSKWRIVIEMVMLINHFLPGVVDLAVRSSVKRIWR
                     THDEHDLQAHATGASHEETGLRKS"
     gene            1974..>2929
                     /locus_tag="VFF70_03095"
     CDS             1974..>2929
                     /locus_tag="VFF70_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZY43712.1"
                     /translation="MNIPKTTSSKPAQGLPNQSGSNNTQASGQLLEKSRLDTDAALKD
                     LDSRLTGLNAAEVDARIKRYGLNEIAREKRTSPLMRLWDNVKNPLVILLIALGVLSYL
                     TGDVRAMIVIFVMVVLGVVLRFFQEMRADSAAEKLKAMVNTTATVVRAGKDAEVPLKL
                     LVPGDIIRLAAGDMVPADVRVVSAKDLFLNQAALTGESLPVEKKATPAVADVQNPLDL
                     QNICFLGSSVESGSAMAIVVHTGDRTYFGSLAASIIGQRQLTSFDKGVNQFTWLMIRF
                     IIVMVPAVFLLNGLSKHDWAQAFLFAMAVAVGLTPEMLPMIVT"
BASE COUNT          715 a          722 c          849 g          643 t
ORIGIN      
        1 atcctattta tcacagcgac acaccggata aagttgcaag gtatatggtg gaaccgtacg
       61 tgatcgcggc cgatgtttac aatgcgccgt cgcacgttgg gcgcggcggc tggacatggt
      121 acaccggctc aagcggctgg atgtatcgcg tgggattgga agcgatcctg ggaatccggc
      181 gaatggggaa agcgttacag atcgatccgt gcattccaaa aacgtggccg agttatcaag
      241 tgacatatcg cgtgggcgaa acgacttttc agatcagcgt ggagaatccg gcgggtgtca
      301 atcgcggcgt caagcaactc aaactggatg gaagtgtttt gaacagcaac gagattccac
      361 tactggacga tggcagtcag catgcggttc acgtgttgct gggcaccgtc accgcaacaa
      421 gctagatctt aggagccagg actttgatgt tatgcagcaa cagtggatca gccgctaagg
      481 caataccggc catcaataaa ctccaccgtt aggtcgagaa gagatcaagg aggaaacaat
      541 gaaaagcgaa atcggtttat ggattgatca ccggcaagca gtgattgtag ttattaccgc
      601 tgccggcgag gaaacaaaac agattgtgtc caacatggag aaacacgttc gattttccag
      661 tggctcgtcc gaggatggtt cacaagaaga cgttcgagat cggcaatttg agaaccgtct
      721 caacagctac tatgatgaag tcgtcgcagt gattcgtcat gccgatgcga ttcaaatctt
      781 tggtccgggt gaagccaagg ttgaactcga aaaacgtctt gagcacgaag ggcttaaggg
      841 acacatcgtg ggtgttgaga cgacggataa gatgaccgat catcagatcg cagcgaaagt
      901 tcgagagcgt ttcccagctt aatttagacg ttatgatagt tattttgaat gaggagcccg
      961 cgctctttag cgcggcggcg ttagagaacg cccgctcctc aaaaagaaaa tcttaaccac
     1021 tatagaaaga ataaaattct catgacgaaa ctcaatgtgg ccggaaaagt aataatcgtt
     1081 acgggtgcgt cagatggcat tgggcgcgaa ctggcgttgc agctgggcgc gcgaggagcg
     1141 atcgtcgttc tcaccgcccg ccgggaacat gtgctggaag aagtcgccac gcgaattcgg
     1201 gcagctggcg gacaagggct ggtcgttccg gccgatttgt gcgacgctaa tctgatcgca
     1261 catctcgtcg agcgcaccgt gcaggaattt gggcgcatcg acgtgctgat caacgtggcg
     1321 ggcatgggat tctatgactg gatcgaggag atgagctccg atgaactcca gcggcagtat
     1381 cagaccaatg tgatcggcct ggccgatctg atccggcagg tagtgccgat catgaagcag
     1441 cagcggagcg ggcacatcat taacttcagt tcctatgcca gccgcatcgc cgtgccaccc
     1501 ttgactatct atgccagcac taagtatgcc atcgaaggct tgagcgacgc actgcgccgc
     1561 gaactggctc cctggggcat ccgcgtcaca cgagttcacc cttgggctgt agacacaaaa
     1621 ttcaatgaaa aagcggcacg gcacagcggg atcatatttc ccgatgaacg tggtgcgcct
     1681 gtcgtagcga tcaccgaggt cacgcgagaa acggtggcga aaagggtgat tgccgcaatc
     1741 gagcgtcccc gccgagcggt ctacgtgagc aagtggcgta tcgtgatcga gatggttatg
     1801 ctgattaatc acttcctgcc gggtgtggtt gatctggctg tgcgttccag cgtgaaaaga
     1861 atatggcgta cacatgacga acacgatctt caggcgcatg ccaccggagc ctcacacgag
     1921 gagacaggac taaggaaatc ctgatcactt aaaaggataa ttctgatcca ctaatgaaca
     1981 ttcctaagac cacctcatca aagcccgcgc agggcttgcc gaatcaatcg ggcagtaaca
     2041 acacgcaggc gtccggccaa ttattggaaa agtcgcgcct ggatactgac gctgcgctga
     2101 aagatctcga ttcgcgcttg acaggactga atgcggcgga agtcgatgcg cgcatcaaac
     2161 gctacggctt gaacgaaatc gcgcgtgaga aacgcacgtc gcctctgatg cgcttatggg
     2221 acaacgttaa gaatcctctg gtcattctgc tcattgcgtt gggcgtgctg tcgtatctga
     2281 ccggcgatgt gcgcgcgatg atcgtcattt tcgtgatggt ggtattgggt gtggtgctgc
     2341 gtttctttca ggagatgcgc gccgatagtg ccgcggaaaa acttaaagca atggtcaaca
     2401 cgactgccac agtcgtgcgc gctggcaaag atgccgaggt gccgcttaag ttattagtgc
     2461 cgggtgacat catccggctg gccgccgggg atatggtgcc cgcggatgtg cgcgtggttt
     2521 ccgccaaaga tttatttctg aatcaggctg cgttgactgg tgagtctctg cccgtcgaga
     2581 agaaagcaac acccgccgtt gctgatgttc aaaatccatt ggatttacag aatatttgct
     2641 tcctgggctc cagcgtggag agcggttccg ctatggcgat cgtggttcac accggggacc
     2701 gcacctactt cggttcattg gctgctagca ttatcgggca gcgccagctg accagcttcg
     2761 acaaaggcgt caatcaattc acttggctca tgatccgctt cattatcgtg atggtaccag
     2821 ccgtcttcct ccttaatgga ctgagcaaac acgactgggc gcaagcgttc ttgtttgcaa
     2881 tggcggtggc tgtcggctta acaccagaga tgctaccgat gattgtgac
//
LOCUS       DASRTJ010000144         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_154920,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000144 DASRTJ010000000
VERSION     DASRTJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154920"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFF70_03100"
     CDS             <1..634
                     /locus_tag="VFF70_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY43713.1"
                     /translation="VSGFFLPDAYTPFEIAMGATVLPQLDEIDPQKFLPSSRSIAFTK
                     EAWRSVNGYPEWLDYCEDLVFDFALLKKYKSFAFAPEAVAYFHPRSSLRSYIQQYYQY
                     ARGDGKADLWLKRHLIRYATYLIGVPLIAWLSLQVSPLLGWLYLIGAIVYLMRPYQRL
                     SSRLSELRSIDRFKAIAWIPMIRVVGDIAKMIGYPVGVLWRLSNSRNQTS"
     gene            667..1395
                     /locus_tag="VFF70_03105"
     CDS             667..1395
                     /locus_tag="VFF70_03105"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZY43714.1"
                     /translation="MKFSVRAKFISIGLLIAIGFSVIRPSIARADDNGPLSNYWKSKI
                     SRWVDLIAANAVARGLDPNLVCSIMFEESKGDPNIVSPAGAVGLMQVMPFPWRPSAEA
                     LKDPAINLTAGTRTLSEVIRQSQGRLHIALMAYNSGWRKLSVPVARSFARKVLEQYAS
                     SILFDAGYDLKKVAGWTMILVSHSSAGPVQADRFRSDGRFEPIIDFDPSIAPEGTPHA
                     LAYATIDPNFIAWWVEVYVYPTLK"
     gene            complement(1564..2349)
                     /locus_tag="VFF70_03110"
     CDS             complement(1564..2349)
                     /locus_tag="VFF70_03110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZY43715.1"
                     /translation="MIDKVTTLAEAALLVNSGDTLSLGGMNLYRRPVAFVRELLKRDV
                     RDLTLLCYSASYESDLLIGARRVTAIRTCYCGLEAFGLAPMFTSMATRGELKVIEESE
                     GSLAFGLRATLGRVGFMPGPGWIGTDMFKVRPDVKVIDDPYTGKPVAAFPAITCDVAV
                     IHAPIADRFGNARLSGNLAVDSDLSLIADRVIITAEEIVGQLDAPMDITGLSVTAVVH
                     APNGAWPTSCYPNYSLDGEEILRYIDTCAAEKFDVYLAEWLRR"
     gene            complement(2352..3020)
                     /locus_tag="VFF70_03115"
     CDS             complement(2352..3020)
                     /locus_tag="VFF70_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HZY43716.1"
                     /translation="MLKFLILDLDETLYPRDAGLMQEIGVRINRYLIDHLHLSEVDAV
                     LLRQRYFLQYGTALRGLIVERSDANPEDYLQFVHDIQLTKYIAPNPALNDMLRSIDLT
                     KVIFTNSTREHAQKVLGILGIADCFSRIIDIRAIEYISKPDRRAYEKMLELLEARPDE
                     CILVEDSARNLVPAKALGLKTILVDSIECDEVDYCVKDILEVKDAVDKWVQVASNKQQ
                     DQKR"
     gene            3222..4226
                     /locus_tag="VFF70_03120"
     CDS             3222..4226
                     /locus_tag="VFF70_03120"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZY43717.1"
                     /translation="MATPEPATGGKFQLPTIIHGPWTQAARQRAVDKECLQHFIEYFG
                     KAMKTRRWSPWHDLPVDEMKQYGDRLTPETVNLIEGFLGIEEYVGDYVLEGLEMFRGN
                     RTRRNLQLQWGSEELKHGVAWEKVLLHSGVRTEEEIEAYCNKVNDHRWSLKNHAGTDQ
                     PLGVTVYAMVQERATFFNYQETRARIRSEYGLPANPTQEELARGYEVGASEGFRVVGV
                     DEIAHHGIFLQIVLTHLKYMPEKALDVMQQVFSGFKMPSLRLIPNARNFIHAVAKTGM
                     HTAERHLKFIHNPVLKSLGLEDNEAFNRAVQAAKLLPEGLGPDQVKLGRTGEFIIAYA
                     "
     gene            complement(4412..5416)
                     /locus_tag="VFF70_03125"
     CDS             complement(4412..5416)
                     /locus_tag="VFF70_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZY43718.1"
                     /translation="MTRLTFSNAPHDIWIVAETIDGQLTPLTKELVYGARMSAGMMGF
                     YVKVALLGSNVDELASELFPVGADHVVVIDDARLKDFSSDIWMQALSDLFAAQSPEFV
                     LFGATSIGDALAPRLAHRFGAGLIGHCISLKVDDFDRCFIGRRSVYSGEYYEVISTAG
                     SKSHFATVLPDTFGEPYPDASRSGEVEKTEVDLNQTVDRVRSLGSIPYELPRKKLKQA
                     RRIVSVGRQVGDVAAAKSLSLSIGAEFAGARESIDEGYIDEDHVIGITGERVAPDLYI
                     ALGIRGDTQHSFGMKDARYVVAVHPDPDAPMLKHADAAIIDDPARVAKELMQLINANQ
                     "
     gene            complement(5466..>5876)
                     /locus_tag="VFF70_03130"
     CDS             complement(5466..>5876)
                     /locus_tag="VFF70_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43719.1"
                     /translation="SLDTACGEVAPHLAEALGLPLILNAAKIEIKEQLLTATVHQSGF
                     LNVESKLPAVVAIMAEAFTGKFANAWRLVDAYKKWNVETWNISDLGLSEEDLQPLTVK
                     KEDAFPPERQFGTQVKNAKELMMMLKRERILSNE"
BASE COUNT         1400 a         1592 c         1510 g         1374 t
ORIGIN      
        1 ggttagcggc ttcttcttgc ctgacgcata cacacctttt gagatcgcaa tgggtgcaac
       61 cgtcctaccg caactggatg agatcgatcc ccaaaaattt ctgccctcga gccgcagcat
      121 cgccttcacg aaagaagcgt ggcgatcagt caacggttat cctgaatggc tggattattg
      181 tgaagacctc gtctttgact ttgctctctt gaagaagtac aaatcattcg cttttgcgcc
      241 ggaagcggtt gcctactttc atccgcgatc ttccctacgc tcatacatcc agcagtatta
      301 tcaatacgca cgtggcgatg gcaaagcaga cttgtggttg aaacggcatc tcattcgtta
      361 tgcgacatat ctcatcggtg taccattgat tgcgtggctc agccttcaag taagtccgct
      421 tttggggtgg ctgtatctca tcggcgcgat tgtttattta atgcgaccgt atcaacgctt
      481 aagcagtcgg ctgtctgaat tgagatcgat cgatcggttc aaagcaatcg cgtggattcc
      541 aatgattcgc gtggtcggcg acatcgccaa gatgatcggg tatccggtcg gtgtgctgtg
      601 gcgattgagc aacagcagaa accagacttc ttgaagaaac cgggtttata gagtaaactc
      661 ttatcaatga aattttctgt acgcgccaaa ttcatctcca tcgggctgct gatcgcgatc
      721 gggttcagtg tgatccgccc atcgatcgcg cgcgccgacg ataacggtcc gctatcgaac
      781 tattggaaga gcaagatcag ccgctgggtg gatttgatcg cggcgaacgc tgtcgcgcgc
      841 gggctcgatc caaatttagt atgctcgatc atgtttgaag aatcgaaagg cgatccgaac
      901 atcgtcagcc ccgcgggcgc ggtcggcttg atgcaagtga tgccttttcc gtggcggcca
      961 agcgccgaag cgttgaagga cccggcgatt aatttgaccg cgggcaccag gacgttgagc
     1021 gaggtgattc gtcagtcaca aggacgtttg catatcgcgt tgatggctta caatagcggt
     1081 tggcgcaaac tctccgtgcc ggtggcgcga tcatttgcac gcaaggtgct ggagcaatat
     1141 gcgagttcga tcttatttga cgccggttac gatttaaaaa aggtagcggg ctggacgatg
     1201 atcctggtca gccacagcag cgcgggtccg gtgcaggccg atcgattccg atcggacggc
     1261 aggtttgaac ctatcattga cttcgatccg tcgatcgcac cggagggcac accgcatgct
     1321 ttggcgtatg cgacgatcga tcccaacttc attgcgtggt gggtggaagt gtatgtctat
     1381 cccacgctga agtaatttgc ttcaggcgtc ggcgcctgtg aaaataaatc ccctgcacaa
     1441 tcacgaccag cactaaaagc acctagatat aagtcgggac gcctaagaaa tcagtgatgt
     1501 cagctaaagt cttcacgagc agcatggatt gcagcatcaa cgctgttcct ttctatggtt
     1561 cgatcagcgg cgcaaccact ccgctaaata aacgtcaaat ttctccgccg cgcacgtatc
     1621 gatgtagcgc aatatttctt caccgtcgag agaataattc ggatagcagg atgtcggcca
     1681 cgcaccatta ggtgcatgca caacggcagt gacgctgagg ccggtaatgt ccatcggcgc
     1741 atcgagttga ccgacgatct cttcggccgt gatgatgacc cgatcggcaa tcagcgacaa
     1801 gtctgaatcg acggcgaggt tgccgctcaa ccgagcgttg ccaaaccgat cggcgatcgg
     1861 cgcgtgaatg acggccacat cgcatgtgat cgccggaaat gcggcaaccg gttttcccgt
     1921 gtatggatca tcgatcactt tcacatcagg tcgcactttg aacatatccg tgccgatcca
     1981 accggggccg ggcatgaagc ctactcgccc tagcgttgcc cgcaggccga aggcaagact
     2041 gccttccgat tcctcgatca cttttaattc gccgcgtgtc gccatcgatg taaacattgg
     2101 cgcaagaccg aacgcttcca aaccacagta gcaggtgcgg attgctgtca cccgccttgc
     2161 gccgatcagt agatcacttt catatgaagc gctgtagcac aacagcgtga gatcgcgcac
     2221 atcgcgcttg agcagttcgc gtacgaaagc gacgggccgt cggtacagat tcatgccacc
     2281 gagagaaaga gtgtcaccgc tatttacgag caaggctgct tcagccagag tagttacctt
     2341 atcaatcata cttatcgttt ttgatcctgc tgcttgttac tggcaacctg tacccattta
     2401 tcaactgcgt ctttcacttc aagaatatct ttcacgcagt aatccacttc gtcacactcg
     2461 atgctatcga ccagaattgt tttcaggcct aacgctttcg caggaacaag attgcgcgcc
     2521 gaatcttcga cgaggatgca ttcgtcgggt cgggcctcga gcaactccag catcttttcg
     2581 taggcgcgcc gatcgggctt gctgatgtat tcgatggcgc ggatatcgat gattcgcgaa
     2641 aaacaatcgg cgataccgag aatgcctaaa actttttgcg cgtgttcgcg cgtagagttg
     2701 gtgaagatca ctttggtcag atcgatcgat cgcagcatgt cattcaatgc cggattcggc
     2761 gcgatatatt tcgtcagttg aatatcatgc acaaactgaa gatagtcttc agggttggca
     2821 tccgatcgtt cgacgatcaa accgcgcaag gctgtgccat attgtaagaa gtagcgctgg
     2881 cgtagtaaaa cagcatcgac ttcagataga tgcaagtgat cgatcaagta gcgattgatg
     2941 cgcacgccga tttcctgcat caatccggcg tcgcgcggat acaaagtctc gtcaagatcg
     3001 aggatcaaaa atttaagcat gaggcgtgta gtttaacgca agaccagcgg cagtgcaaac
     3061 ccgcagctga aattgcaaaa tcaaatatga tataattccg actgcgaagc gatattcagc
     3121 ctgacgtctc tccatcgttc gactgctcat ttaccaccga gccattgggt tgccgcctct
     3181 atcgctcccc gcaccatctt tgatttagat cggactagaa catggcaaca cctgaacccg
     3241 cgaccggcgg gaaatttcaa ttaccgacca tcatccatgg gccgtggaca caagccgccc
     3301 gccagcgcgc cgttgataaa gaatgcttgc agcatttcat cgaatatttc ggcaaggcga
     3361 tgaagacgcg ccgctggtcg ccgtggcacg atctgcctgt cgacgagatg aagcagtacg
     3421 gcgatcggtt gacgcccgaa acggtcaact tgatcgaagg ctttctcggc atcgaagaat
     3481 atgtcggcga ttatgtgctc gaagggctgg aaatgtttcg cggtaatcgc acgcgccgca
     3541 atttgcaatt gcagtggggc tcggaagagt tgaagcatgg cgtggcctgg gaaaaagtgc
     3601 tgctgcattc cggtgtgcgc acggaagaag agatcgaagc ctactgcaac aaagtcaacg
     3661 atcatcgctg gtcgctgaag aatcacgccg gaaccgatca accgctgggt gtaacagtct
     3721 atgcgatggt gcaagagcgc gcgacattct ttaactatca agagacgcgc gcccgcatcc
     3781 gatcggaata cggcttgccc gccaatccga cgcaggaaga actggcgcgc ggctacgaag
     3841 tcggcgcgtc ggaagggttc cgcgtcgtcg gcgtggacga gatcgcgcat cacggcatct
     3901 tcttacagat tgtcttgacg catctcaagt acatgccgga gaaggcgctc gatgtgatgc
     3961 agcaagtctt cagcggcttc aagatgccgt cgctgcgctt gatcccgaat gcgcgcaatt
     4021 tcattcacgc cgtcgccaag accggtatgc ataccgccga acgtcatttg aaatttattc
     4081 acaatcctgt actgaaatcg ttggggcttg aagacaatga agccttcaat cgagcagtac
     4141 aagcagccaa gttgctgccg gaaggactcg gacccgatca agtgaaactg ggccgcacag
     4201 gtgaattcat catcgcctac gcgtaagtga gtggaaacaa aaaactcgct tccaaaaccg
     4261 gaagcgagtt ttttattttc ggtaaacgtc attgcgagcc gcgctctttg cggcgaagca
     4321 atctcctcta caatcaactg agattgcttt gtcgtgcacc gcactcacct gccctgtctg
     4381 ctttttacag ggtcgcaatg acggatcaca ttcactgatt cgcgtttatc aactgcatca
     4441 actctttcgc cacccgcgct ggatcatcga tgatagctgc atcggcgtgc ttcaacatgg
     4501 gcgcgtcggg atcgggatgg acggcgacga cgtagcgagc atctttcatc ccgaatgaat
     4561 gttgcgtatc gccgcgaatg ccgagcgcga tgtaaagatc gggcgcaacg cgttcgcccg
     4621 tgatgccgat cacatgatct tcatcgatgt agccttcgtc gatcgattca cgcgctccgg
     4681 caaactcagc cccgatcgat agggacagcg acttcgcggc tgctacgtcg ccaacttgtc
     4741 tgcccaccga cacgatcctc cgcgcttgtt tcaatttctt tcgcggcaac tcatatggga
     4801 ttgatcccaa cgaccgtact cgatccaccg tttgattcag atcaacttca gttttctcca
     4861 cctcaccgga tcgcgaagca tctggatacg gctcaccaaa tgtatcgggc aagactgtgg
     4921 caaagtgcga cttcgatcca gcggtcgaaa tcacttcgta atattcaccg ctgtaaaccg
     4981 atcgacgtcc gatgaagcat cgatcaaagt catcgacctt caatgaaatg cagtggccga
     5041 tcaagcccgc gccgaagcgg tgcgccagtc gcggggcaag cgcgtcgccg atcgacgtcg
     5101 caccgaacag cacgaactcg ggcgattgag cggcgaacaa atcactgagc gcttgcatcc
     5161 aaatgtcgct cgaaaaatct ttcagtcgcg catcatcgat cacaaccacg tgatccgcgc
     5221 cgacagggaa caactcacta gcaagctcat ccacattcga gccaagcaac gccactttca
     5281 cgtagaagcc catcatgccg gccgacatgc gcgcgccgta cacgagttct ttcgtgagcg
     5341 gcgtcagttg tccgtcaatc gtttcggcga caatccaaat atcgtgaggc gcattggaaa
     5401 aagtaagtcg tgtcatagtt agtccttgaa cattaacgag taaaagcggc tcgcccaatt
     5461 actcattact cgttactcag tattctttct ctcttcaaca tcatcatcaa ctctttcgca
     5521 ttcttcactt gcgtgccaaa ttgtctttcc ggcgggaaag catcttcctt ctttacagtc
     5581 aacggctgca aatcctcttc gcttaatccc aagtcggata tattccaagt ttcgacgttc
     5641 catttcttgt aggcatcgac caaacgccaa gcattggcaa actttccggt aaaggcttcg
     5701 gccatgatcg caacaaccgc cgggagtttt gattcaacgt tgaggaaacc agattgatgc
     5761 actgtcgccg tcaacaattg ctctttgatt tcaattttgg ccgcgttcaa tatcaaaggc
     5821 aggcctaacg cttcggccag gtgcggcgcg acttcgccac acgcggtgtc taggga
//
LOCUS       DASRTJ010000145         7386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_235254,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000145 DASRTJ010000000
VERSION     DASRTJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235254"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1770)
                     /gene="nuoF"
                     /locus_tag="VFF70_03135"
     CDS             complement(40..1770)
                     /gene="nuoF"
                     /locus_tag="VFF70_03135"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZY43720.1"
                     /translation="MSDLDLTALQLAIIEFIPQGRSGLLPALHAAQLIYGYLSEPVAA
                     EIARLLNVPLADVYGVIDFYSMFYREPIGRTVVRICTDPSCALLGADEILEAACHHAG
                     VAVGDTSKDGAYAVERSTCLGLCNVGVAVNVSRSDSAKSFAHVTTNAVNDLFEMRGHA
                     ADDFVGGYVRGITALCDRSNAATLTEYEAHGGIAALRKVLVGGMSQADLIDEIKRSGL
                     VGRGGAAFPMWIKWDGALKSKVEGSRFFVVNADESEPGTFKDRILMERDPQRFIEGAI
                     LGAYAIGAHQAYIYIRGEYPTAIERVNNALVELKAHGYLGKNILGSGFDLEVEIRSGA
                     GAYICGEETALFESIEGKRGFPRVKPPFPTEHGLFGQPTAINNVETLANVPTIIANGA
                     DAYKKFGTEKSSGSKLFCLSGDVAKPGLYEVPFGVTLRHLINDLAGGVSGGMQAILMG
                     GAAGSFATEKDLDVKLSFEDMRAAGLPLGSGAIMVFNQSRDLRDVLLRLGRFFAHESC
                     GKCYPCQLGTQRQYEVLQRVASGKTQSGDVDRLSDVTWTMSDASLCGLGQTAGWAVQS
                     AMKLWPEMFR"
     gene            complement(1995..3836)
                     /gene="glmS"
                     /locus_tag="VFF70_03140"
     CDS             complement(1995..3836)
                     /gene="glmS"
                     /locus_tag="VFF70_03140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZY43721.1"
                     /translation="MCGIVGYVGPRNATPIIYDGLKHLEYRGYDSAGIAVIQNGSIEV
                     RRDAGKLDNLRSLLDESPISGSIGIGHTRWATHGQANQINAHPHLDCSGDLVVIHNGI
                     VENFLDLRHELRAKGHTFRSETDTETIVHLVEQYLIDGHDFEEAARLTLNRLKGAQAV
                     VLLNKRQPDRIIAARIGNAGGVTLGLGTGEMFIASDMPAILDYTREMVFLESKQMATV
                     LRDGYKVMTLDGTRIEPEVHHVPYDPTSAAKGEYRHFMQKEIFEQARSLTDTIRGRAD
                     FDRGAIDLPDLHLTPEVAKSIDRLMILACGTSWHAALVGRFMIEALARIPVEVDIASE
                     FRYRDPVIDSSTAVLAITQSGETVDTLAAMELARERGTKLWSIINNVGSQAMRAADGH
                     ILIQSGPEIGVASTKTFTGSQVDQYMLGAYLGQLRGTLNGDRLRSVVADLARLPDLVG
                     QLFDRTGAIESLANEFYRYEDFLFLGRGINYPIALEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDENMPIVCLAPKDGVYDKMISQIEQAKARGGIVIGVATDGDEDIRRKADHV
                     IYVPPAPALLNPILLSIPMQLLAYYIAVRRGADVDQPRNLAKSVTVE"
     gene            complement(4261..5670)
                     /locus_tag="VFF70_03145"
     CDS             complement(4261..5670)
                     /locus_tag="VFF70_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HZY43722.1"
                     /translation="MQTQLKQDQHGGIIAAIEPDSAAAEMKLHIGDVVIEVNGHPVED
                     VIDVQYYAADEWIELKIRRGETVLSFEGPRDYNQALGIEFEHPTFDIDIRRCNNLCSF
                     CFVLQMPNRMRHALYIKDDDYRYSFLHGHFVTLTNLAQHDWDRIAEQHLSPLYVSVHA
                     TDLEVRRKLLRNPVAPDILVQLRWFKEHGIEVHTQLVVTPGLNDGKYLSRSIHDLAKL
                     WPTVQSISVVPVGITKSHKYNERPCTLEESRLIVDEVHRYQEEFLKKFGKRFAYLTDE
                     WYLVLGEQVPPKSYYDKLSLEENGLGMVRNFLNDWRAVKLEVAQRKRFKYKSATLVTA
                     TLFAPTLTQVAKQFTRVSGWKIEVIPVINERLGATITVAGLLMGEDVIAQLEGRELGG
                     IVVLPRIMFDHPRGVSLDDKSVLDVSRALDRPVALADAMGDVLDALTGRNRLTVMPTD
                     QFIPSEVMMAGGWAVEKYL"
     gene            complement(5845..6585)
                     /locus_tag="VFF70_03150"
     CDS             complement(5845..6585)
                     /locus_tag="VFF70_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZY43723.1"
                     /translation="MIESSQAKIKNQKSGIGNLFINYKPRLRRRLLTFGLQAAMKLLF
                     HIESVGWEHVPASGGLILMINHIAFLDPVLLTGLFPRPIISMAKAEALDEKIVGPLIK
                     SFDAFPVNRGTGDRQALRTAFEVLAAGLPLLIAPEGHRSESVTLGEAHEGIAFIASRS
                     QVPVVPVGISGTDQFKHNFKRWRRTPAIYRFGKPFILDAGDERASSDVLKRMTDEAMY
                     QLASLLPLEQRGIYSNAEAMTTQYVRFV"
     gene            complement(6694..>7386)
                     /gene="cmk"
                     /locus_tag="VFF70_03155"
     CDS             complement(6694..>7386)
                     /gene="cmk"
                     /locus_tag="VFF70_03155"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZY43724.1"
                     /translation="IFMRPTTIAIDGPAASGKSTLGAALAQHLGYLYFDTGVMYRAVT
                     WAALQRQIDIEDELAVTRLAEAVSIDVRPPSKTDGRQYDVICDSEDVTWAIREPRVDQ
                     YVSPVSVYPGVRSAMTAQQRRIGLQGNVIMVGRDIGTVVLPEAQLKIYLDATVEERAH
                     RRYHEVQTRGNHASAQTFDEILAAMQRRDAIDTGRATAPLKPAVDAVIIDSTQMDVPH
                     VIAAVEKLINSD"
BASE COUNT         1723 a         2140 c         1835 g         1688 t
ORIGIN      
        1 gatttcactt gtcgctaatt attaaccact aactcctttt caccggaaca tttccggcca
       61 aagtttcatc gcactctgca cggcccaacc cgccgtctga cccaggccgc atagcgacgc
      121 atcactcatc gtccacgtca cgtcgctcaa gcgatcgacg tcgccggatt gagtcttacc
      181 actcgccacg cgctgcaaaa cttcgtactg acgctgtgtg ccgagttggc acggataaca
      241 tttaccgcac gactcatgcg cgaagaatcg ccctagtcgc agcagcacat cgcgcagatc
      301 gcgcgactga ttgaaaacca tgatcgcgcc agaaccgagc ggcaatccag cggcgcgcat
      361 gtcttcaaaa gacaatttca catccaaatc tttttcggtc gcgaatgaac cggccgcgcc
      421 gcccatcaag atcgcttgca tgccgccgga aactccgcca gccaaatcgt tgatcaaatg
      481 tcgcagcgtg acgccgaacg gcacttcata aagtcccggt tttgctacat cccccgataa
      541 acagaacaac ttcgacccgc tcgacttttc ggtgccgaat tttttgtagg cgtctgcacc
      601 attcgcaatg atagttggca cattcgctaa cgtctcgacg ttgttgatcg ccgtcggttg
      661 accgaacaaa ccgtgctcgg tcgggaatgg cggcttgaca cgcggaaagc cgcgcttgcc
      721 ctcgatcgat tcgaatagcg ccgtctcttc gccgcatata tacgcccccg cgcctgatcg
      781 tatttcgacc tcgagatcga aacccgatcc caaaatgttt ttgcccaaat atccgtgcgc
      841 tttcaattca accaaggcgt tgttgacgcg ctcgatcgcc gtcgggtatt caccgcgaat
      901 gtagatatac gcttggtgtg cgccgatagc atacgccccc aagatcgcgc cttcgatgaa
      961 gcgctgcgga tcgcgttcca tcaagattcg atctttaaac gtgcccggct cggattcatc
     1021 cgcattgacc acaaagaatc ttgatccttc aactttcgat tttaacgcgc cgtcccattt
     1081 gatccacatc ggaaaggccg ctccgccccg ccccactaaa cccgatcgtt tgatctcgtc
     1141 gatcaaatct gcttgactca tgccaccgac aagaactttt ctcagcgcgg cgattcctcc
     1201 atgggcttcg tattccgtta atgtcgcagc attcgatcga tcgcacaacg ccgtgatccc
     1261 gcgcacataa ccgccgacaa aatcatcggc tgcatgtccg cgcatttcaa acagatcgtt
     1321 gacagcgttg gttgtaacat gtgcaaatga ttttgccgaa tcggatcgag agacgttgac
     1381 tgccacgccg acattgcaca gacccaagca agtcgatcgt tcgacagcat aagcgccatc
     1441 ctttgaagtg tcgccaactg cgactccagc gtgatgacat gccgcttcca agatttcatc
     1501 cgcgcccaac aacgcgcatg acggatcggt acagatgcga acgacggtac ggccgatcgg
     1561 ttcacgataa aacattgagt aaaaatcgat tacgccatag acatcggcca gcggcacgtt
     1621 caacaatcga gcgatctcag cagcgactgg ctccgacaaa tatccgtaga tcaattgagc
     1681 agcatgcaaa gcgggcagca ggccggatcg accttgagga atgaattcga taatggcaag
     1741 ctgaagagcg gtcagatcaa gatcagacat tgcgcctccg ttggctgaat tgagggactt
     1801 agattgtaaa ttcaaatcag gcgctggtca aatggcgaga atgcgcaatc attttatacc
     1861 aactaccaag taggaaacaa agtagcggcg agcctcatac tcgccgctac attattcaag
     1921 aaattttctt gttacctgat cactccaccg tcacactccc gatagggcca aattccttga
     1981 catcgatcaa ttgatcactc aacggtcacc gacttcgcca aattcctcgg ctgatcgaca
     2041 tccgcgccgc gccgcacggc aatgtagtag gccagcagct gcatcgggat cgataacaga
     2101 attggattca acaatgctgg cgcaggcggc acgtagatca catgatcggc tttgcgccgg
     2161 atgtcttcgt cgccatcggt cgccacgccg atcacaattc caccgcgcgc ttttgcctgt
     2221 tcgatctggc taatcatttt gtcgtatacg ccatccttcg gcgcgaggca gacgatcggc
     2281 atgttctcgt caatcaacgc gatcggcccg tgcttcatct cgcccgcggg atagccttca
     2341 gcgtgaatat atgaaatctc tttcaacttc aatgcgcctt cgagtgcgat gggatagttg
     2401 atgccgcgtc cgagaaacag aaaatcttca tagcggtaaa attcattcgc caaagattcg
     2461 atcgccccgg ttcgatcgaa gagctgcccg acgagatcgg gcaagcgcgc cagatcggca
     2521 acaaccgaac gcaatcgatc gccgttcaac gtgccgcgca gttgacccaa atacgcgccg
     2581 agcatatact gatcgacttg cgaaccggta aaggttttgg tcgaagccac gccaatctcc
     2641 ggccccgatt ggatcaggat gtggccatcg gccgcgcgca tcgcttgcga gccgacgttg
     2701 ttgatgatgc tccacaattt ggtgccacgc tcgcgcgcca gttccatcgc cgccagtgta
     2761 tcgaccgtct caccactttg ggtgatcgcc aacaccgccg tcgaagaatc gatcaccgga
     2821 tcgcgatagc ggaactcgga ggcgatatcg acttcgaccg gaatacgtgc aagcgcttca
     2881 atcataaagc ggccaaccaa tgccgcatgc caagatgtgc cgcacgccag gatcatcaat
     2941 cgatcgatcg atttggcaac ctcgggcgtg agatgtagat caggcagatc gatcgcaccc
     3001 cgatcgaaat ccgcgcggcc acggatcgta tccgtcaacg atcgggcttg ctcgaaaatt
     3061 tctttctgca tgaagtggcg atattcgccc ttggcagccg acgtaggatc gtatggcacg
     3121 tgatgcactt ccggttcgat ccgagtgcca tcgagcgtca tgactttgta accatccctc
     3181 aacacggttg ccatttgctt cgattcaaga aacaccatct cgcgcgtata atcgagaatc
     3241 gccggcatat ccgaagcgat gaacatctcg cctgtgccaa ggcctaacgt caccccgcct
     3301 gcgttgccga tgcgggccgc gatgatccga tcgggttgac gtttattcaa caacaccacg
     3361 gcctgtgcgc ccttcaatcg attcaacgtg agacgcgcgg cttcttcaaa gtcatggccg
     3421 tcaatcaaat attgttcaac cagatgcacg atcgtttccg tgtcggtctc gcttcgaaag
     3481 gtgtgaccct tcgctcgcag ttcgtggcgt aggtcgagga aattttcgac gatgccgtta
     3541 tggatcacga cgaggtcgcc cgaacaatcg aggtgtggat gcgcgttgat ttgattcgct
     3601 tgcccgtgcg tcgcccagcg cgtatgccca atgccgatcg atccgctgat cggcgattca
     3661 tccaaaagag atcttaaatt atccaacttg cctgcgtcgc gccgcacttc gatcgatcca
     3721 ttttggatca cggcgatgcc cgcggaatca taaccgcgat actctaagtg tttcaagccg
     3781 tcgtaaatta tcggcgtcgc attacgcggg ccgacatagc caacaatgcc acacatgaga
     3841 aacccctccg ttggggaatt aatttcgtag cggcagacct atgtgtctaa cctgggcgaa
     3901 cacgccggtt cgcatcctac gatttcgatc gccactacgc tgcaatttgt ctcatgagtc
     3961 acctcatgca tttatcgccg cgctttaaat cattggaggg gaaagaatta ggatcgtggc
     4021 tcgaccctga cggcatccgc tgatacatcg atgtttctgg ctgatcgcca gttagctctg
     4081 tcctgcgaca ggaaccgtcg cctcgtcaac tgatcacctc gatcagtcca tgcgctgttt
     4141 cccctgatta ttttttcgat ctacctcctg cttatttttg tcggagcgac gagattatac
     4201 tcgatttcag atttttcgtc acgacgattg aactacggat gaccgatcgt tttcctacgc
     4261 ttacaaatat ttttcgacgg cccagccacc agccatcatc acctccgacg gaataaactg
     4321 atccgtcggc atgaccgtca atcgattgcg gcccgtcagc gcatccagca catcgcccat
     4381 ggcatcggcc agcgccacag gtcgatcgag cgcgcgcgat acgtccaata ccgacttgtc
     4441 atcaagcgaa acgccgcgcg gatgatcgaa catgatacgc ggcagcacca cgattccacc
     4501 cagttcacgc ccttcaagtt gagcgatcac gtcttcgccc atcaacaaac cggcgacggt
     4561 gatcgtcgcg cccaagcgtt cgttaatgac tggaatgact tcaatcttcc agccgctcac
     4621 gcgcgtgaat tgtttggcta cttgggtcaa agtcggcgcg aacaaagtag cggtcactaa
     4681 cgtggcgctc ttgtacttaa agcgtttgcg ctgagcgacc tcgagtttga cagcgcgcca
     4741 gtcattcaaa aagttgcgta ccatgcccaa gccgttctct tcgagcgata atttatcgta
     4801 ataagactta ggtggaactt gttcgcccag gactaggtac cactcatcgg taagatacgc
     4861 aaagcgcttg ccgaacttct tcaagaattc ttcctgatag cgatgcactt cgtcgacaat
     4921 caaccgcgat tcttcaagtg tacagggtcg ttcattgtat ttgtgcgatt tggtaatacc
     4981 caccggcacc acactgatcg attgaaccgt cggccagagt ttcgccaggt cgtggatcga
     5041 tcggctgaga tacttgccgt cattcaaacc gggcgtcacc actaattgcg tgtgcacttc
     5101 aatgccatgt tctttgaacc agcgcaattg aacgagaata tcaggcgcga caggattgcg
     5161 cagcaatttg cggcgaactt ccagatcagt ggcatggacg gaaacataga gcggcgagag
     5221 atgctgctcg gcaatgcgat cccaatcgtg ctgggccaga ttcgttaaag tcacaaagtg
     5281 gccgtgcagg aaagagtaac ggtaatcatc gtctttgata tacaatgcat gccgcatgcg
     5341 attcggcatt tgcagcacga aacaaaacga acacaggtta ttgcagcgcc ggatgtcgat
     5401 gtcgaaggtg ggatgttcga attcaatgcc gagcgcttgg ttgtaatcgc gtgggccttc
     5461 aaagctcagg actgtttcgc cgcgcctgat cttcaactcg atccattcat cggcggcgta
     5521 atactgcaca tcgatcacat cttcgaccgg atggccgttg acttcgatga cgacatcgcc
     5581 gatgtgcaat ttcatttcag cggcggcaga gtcgggttcg atggcagcga tgatgccgcc
     5641 gtgttgatct tgtttcaatt gagtttgcat ttcaccgcca agacgcaatg agcgcgaaga
     5701 gacaggtaac atgtcattgc gagccgccga tcccgcaaac aacgggcggg accttcgggg
     5761 cggcgtggca atcgctatcg ccatcagcta agattgcttc gtcgcgcttc gcgctcctcg
     5821 caacgacgaa taggctcttc gcactcaaac gaatctcaca tactgtgttg tcatcgcttc
     5881 ggcatttgag tagatgccgc gttgttccag tggaagtaat gacgccaact gatacatcgc
     5941 ttcatcggtc atgcgcttca acacatcact gctggcgcgt tcatcgcccg catccaaaat
     6001 aaacggtttg ccgaagcgat agatcgcagg tgtgcgccgc cagcgcttga agttgtgttt
     6061 gaattgatcg gttccactga tgccgactgg caccaccggc acttgtgatc gactggcgat
     6121 aaacgcgatg ccttcatgcg cttcacccag cgtgaccgat tcactgcgat ggccttcagg
     6181 cgcgatgagc aacggtaatc cggctgctaa cacttcgaaa gcagtccgca atgcctgtcg
     6241 atctccagtg ccgcgattga ctgggaacgc atcgaaggac ttgatcaatg gaccaacgat
     6301 cttctcatcg agtgcttccg ctttcgccat ggagataatc gggcgcggaa acagaccggt
     6361 cagcagcacc ggatcgagga aggcgatatg gttgatcatc aagatcaagc ctccgctggc
     6421 tggaacatgc tcccaaccca cgctctctat atgaaacaac aatttcatcg ccgcctgtag
     6481 gccaaaagtc aacaggcgtc gtcgcaggcg gggcttatag ttgatgaaca gattgccgat
     6541 gcctgatttt tgatttttga tttttgcttg agatgattcg atcataaact ggtcgtgtca
     6601 ttccgagcgc agcgaggaat ctctagcacc attcgtgaac aatcccagag atttctcgcc
     6661 tcactccgcg aggctcgaaa tgacattcga tcttcaatca ctattaatca atttctcaac
     6721 agcggcgatc acatgcggga catccatttg agttgaatcg ataatcactg catcaaccgc
     6781 gggcttgagc ggcgcggtgg cccggccagt atcgatcgcg tcgcggcgct gcatggcggc
     6841 cagaatctca tcgaaagttt gcgcggaagc gtggttgccc cgcgtttgaa cttcatgata
     6901 acgtcgatgc gcacgctctt ccacagtggc atccaaataa atcttgagtt gggcttcggg
     6961 caaaacgacc gtgccgatgt cgcgcccaac catgatcacg ttgccttgta aaccgatgcg
     7021 ccgctgttgg gcggtcatgg ccgatcggac gccgggataa accgaaacag gcgacacata
     7081 ttgatcaacg cgcggctcgc ggatcgccca cgtcacatct tcactatcac agatcacatc
     7141 gtattggcgt ccatcggttt tcgatggcgg ccgcacatcg atcgagaccg cttcggccaa
     7201 gcgggtgaca gcgagctcgt cctcaatatc gatctggcgc tgcagcgccg cccacgtcac
     7261 ggctcgatac atcacacccg tatcgaaata caaatagccc aagtgctgcg ccagtgccgc
     7321 gccaagcgtg ctcttgccgg acgcggccgg cccatcaatg gcaatcgttg ttggtctcat
     7381 gaatat
//
LOCUS       DASRTJ010000146         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117103,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000146 DASRTJ010000000
VERSION     DASRTJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117103"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFF70_03160"
     CDS             complement(<1..605)
                     /locus_tag="VFF70_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY43725.1"
                     /translation="MNFLVTGGAGFLGAALANRLVLSGHTVRVLDDLTTGDPARLDPR
                     VLFTRGDVTDVPKLWSLLQSIDCVYHLAARVSVPESILYPRDYNAVNVGGTVAVMEAI
                     RDAGVKRVVLASSGAIYGTQQHQPVHEDLPPNPDSPYAVSKLAAEHYVRTIGALWKIE
                     TVCLRIFNAYGPGQPLPPSHAPVIPQYLRQALNNGSLVMFG"
     gene            766..2136
                     /locus_tag="VFF70_03165"
     CDS             766..2136
                     /locus_tag="VFF70_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43726.1"
                     /translation="MRTSFKNILKRIDILLPRALIFALMALMVGQEYPTPGDYRTQIL
                     RAVGDRFFDFVRWEGEAVTEKIFQLAVPIQNYLDDRQQEQFVLDYLELTQQWMSLVQK
                     VRDIYADASIKDPVSASADLRSQRAAARRRIEQQRPTAEAIIQQQISSVLIDDGFAVG
                     GEVLPPVAARITPLPYMLIVSSRDQIKQIDAEGLQAGLNVDQAEQIEAQVLSMTNSSA
                     LVVPIGGLAAYPTMVLETSQLLWLLQTSSHEWTHNWLELRPLGYSYLASDNGEIRTIN
                     ETVASLVGDEVGLQVMRRYYLDVLKRDYPDLVDPNPLQIPQPDQSTAADRPSDLNQFS
                     FNRAMHDTRVHVDALLAEAQSLEKQNQNDQADAKIVEAEQYMEEQRQLINSHGYRIRK
                     INQAYFAFYGAYADQPGASGSDPIGPNVIALRVYSPDLRAFLDRVSSILKLDDLKQAV
                     EALKPH"
BASE COUNT          552 a          618 c          560 g          502 t
ORIGIN      
        1 tcaccaaaca tcaccaatga tccgttattc aatgcttgcc gcaaatattg tggaatgacc
       61 ggggcgtgcg agggaggcag cggttgaccg ggaccataag cattgaagat gcgcagacac
      121 accgtttcga ttttccacaa ggcgccgatc gtgcgcacgt agtgctcggc ggcaagtttg
      181 ctgactgcat acggcgaatc aggattcggc ggcaagtctt catgcaccgg ctgatgttgc
      241 tgcgtgccgt agatcgcgcc ggacgaagcc agcactacgc gcttcacgcc cgcatcgcga
      301 atggcctcca tcacagccac ggtaccgcca acattgaccg cattgtaatc acgcggatac
      361 aagatcgatt cgggcacgga tactcgcgcc gctaagtgat agacacaatc gatgctttgc
      421 aacaacgacc acagcttagg cacatccgtc acatcgccgc gggtgaacaa cactctcgga
      481 tcgagtcgcg ccggatcgcc ggtcgtcaga tcgtccagca cgcggacggt atgtccgctg
      541 agcaccaatc gatttgctaa cgccgcgccg agaaaacctg cgccgcccgt aaccagaaaa
      601 ttcataaatg atcccaaatg agattgatca agcagcgcga ttatagcatg gtatccaact
      661 gcggcctgcg ggttgttgca ctcaacacat ttgcaagcga gttataatta cgctgacttt
      721 tgagggagaa tgtaattagt gtctaaatta cactaaaccg agcatatgcg gacttcattt
      781 aaaaacattt tgaaacgaat cgatattctg ctgccgcgtg cgctcatctt cgcgttgatg
      841 gcgctgatgg tcggccaaga atatccgacg cccggtgatt accgcacaca aatcttgcgc
      901 gcagtgggcg atcggttttt cgattttgtg cggtgggaag gcgaggcagt caccgaaaaa
      961 atttttcagc tcgccgtgcc gatccaaaac taccttgacg atcgccagca agagcaattt
     1021 gtgctggatt acttggagct tactcaacag tggatgtcgc ttgtacaaaa agttcgtgac
     1081 atctacgctg acgcatcgat caaagatccg gtcagcgcct ccgccgatct tcgatcacaa
     1141 cgcgctgcgg cccgccgccg gatcgagcag cagcggccta ccgccgaagc gatcattcaa
     1201 caacaaatct ccagcgtatt gattgatgat ggttttgccg ttggtggtga agtcctgccg
     1261 ccggtcgcgg cgcgcattac gccactgccg tatatgttga tcgtttcgtc gcgtgatcag
     1321 attaagcaga ttgatgcgga aggtcttcag gccggattga atgttgatca agctgagcag
     1381 atcgaagcgc aggtattgag catgacgaac tcttcggcgc tggtcgtgcc catcggtggg
     1441 ctggccgcct atccgaccat ggtcttggag acatcacaac ttctctggtt attgcaaacg
     1501 tcctcgcatg aatggacgca caattggctg gaactgcgcc cgctcggtta cagttatctc
     1561 gcctctgaca acggcgagat tcgaacgatc aacgaaaccg tggcctcgct cgtcggcgat
     1621 gaagttggct tgcaagtcat gcgtcgatac taccttgatg tgttgaagcg cgattaccca
     1681 gacttggtcg atcccaaccc tttgcaaatt ccacagcccg atcaatccac cgcagccgat
     1741 cggcccagcg atctgaatca atttagtttt aatcgcgcca tgcatgacac ccgagttcac
     1801 gttgatgcgt tgttagctga agcccaatca cttgaaaaac aaaatcaaaa tgatcaagcc
     1861 gatgccaaaa ttgttgaagc cgaacaatat atggaagaac agcgacagct gatcaactcg
     1921 catggttatc gcatccgcaa aatcaatcaa gcctacttcg ctttctatgg cgcgtatgcg
     1981 gatcagccgg gcgcgtcggg cagtgatccg atcgggccga acgtgatcgc tttgcgcgtc
     2041 tacagcccag acctgcgtgc ctttctcgat cgagtctcca gcatcctcaa acttgatgac
     2101 ttaaagcaag ccgttgaagc actgaagccg cattgacttc aatgcatctg aacttccaca
     2161 aatcacttcg gcaactcact aaaaacaaaa aacgctactc tcatcagcgc agcgtttcta
     2221 cctcacccat tg
//
LOCUS       DASRTJ010000147         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_8462,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000147 DASRTJ010000000
VERSION     DASRTJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8462"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..879
                     /locus_tag="VFF70_03170"
     CDS             262..879
                     /locus_tag="VFF70_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZY43727.1"
                     /translation="MADQVVPSRGITRREFLNYVALASIALFTAEVGGAVLAFSLPRF
                     REGEFGGTRTIGTVGDKLPVADSGPVAYADIKTWIVNVGPDAASKGGGKQGLVALYKV
                     CVHLGCLYAWVDSNHRFECPCHGSKYQLDGTYLEGPAPRDLDRFAVIIKDDQGATIAQ
                     TVTTPGDPDYGAPIQIPANAGNYTIVVDTGNKIDLGKRHYYTGGA"
     gene            883..>2378
                     /locus_tag="VFF70_03175"
     CDS             883..>2378
                     /locus_tag="VFF70_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZY43728.1"
                     /translation="MRLEPFATLNDMRKQYGIRKTILIKVDESVERFTAGLRINDIRG
                     VLRGDPPGKPNPRVKPHTEGAWFHIWPTFYHEAVTKFYPTFKLGMITTIFFIIEIITG
                     LFLMLYYTPAPGISYDNMLRILSNVPLGQFTRDLHRLAAEGMVIAVWLHMGRTFLTGS
                     YKKPRQFTWATGVILLLLTIGLSYTGYLLPWDQLAYWAVTIGASMADAAPPPEVGKAV
                     GLLLKGGPDLGAGGLLRFYLLHVFAFPLVAIIFVFVHYYKVIRHGHSLPPENETIGED
                     NARKVPPDKRVPFLPDIFTNELLWFGFIMAAMIVPIALNLYHAPLEHAANSQSTPLHT
                     VAPWYFLFLQGWLKLGDKTFWGVIVPTILIGLLMFWPYIDYNPSRRYGDRKIALMAMM
                     FVIGILVVSSYMGTPGFSVVTAPQLEVGFHALPPEQPGPIRSLPFDQLPVGTWSTYDL
                     SGIPDNANELKAVMADISHEIDAEPKSIVSNGVEVTYQSMHAELTIEV"
BASE COUNT          489 a          639 c          684 g          566 t
ORIGIN      
        1 ggtgatcaaa ctgggtggtt gaagttcggg catgatggtt ggccgtgttc gtggtctgcg
       61 gcctattatc gaggcgagtg cgatcaaagt caagaatgat cgcgaggcga atcttcaaaa
      121 tgatttgaca tgatcatccg attgatatat atttagcccg ccagcaattg cgtcggggga
      181 aaataaatcg ggtgcggtca gcccgatttt gtataccccg cccagtttga gcagtcatcg
      241 tattcaggag gattaaccgt tatggcagat caagtggtac cttcgcgcgg catcacccga
      301 cgcgaattct tgaactatgt ggcactggcc tcgatcgcct tgttcacggc ggaagtgggc
      361 ggcgcggtct tggctttctc gctgccgcgt tttcgtgaag gcgagttcgg cggcacgcgc
      421 accatcggca cggtgggcga taagttgcca gtcgccgaca gcgggccggt agcatatgcg
      481 gacattaaga cgtggatcgt taacgtcggg ccggacgccg ccagcaaagg cggcggcaag
      541 caaggcttag tggcgttgta taaagtctgt gtgcatctgg gctgtttgta cgcctgggtc
      601 gattcgaatc atcgctttga gtgcccatgc cacggctcga agtatcagct ggatgggact
      661 tatctggaag gcccggcccc gcgtgatctt gatcggtttg ccgtgatcat caaagatgat
      721 cagggtgcca ccatcgctca aactgtcacc acccccggtg atccggacta cggtgcgccg
      781 atccagattc cagccaatgc cggcaactac accattgtgg tcgataccgg caacaagatc
      841 gatctgggta agcgacatta ctatacggga ggggcataag gcatgcgact cgaacccttt
      901 gcgaccttga acgatatgcg gaagcagtac ggcatccgca aaacaatttt gatcaaagtg
      961 gatgagtcgg tcgaacgctt caccgccggt ttacggatca acgatattcg cggcgtgctg
     1021 cgtggcgatc cgcccggcaa acctaacccg cgcgtgaagc cgcacaccga aggcgcgtgg
     1081 ttccacattt ggccgacgtt ctatcacgaa gccgtcacca agttttatcc caccttcaag
     1141 ctggggatga tcacgacgat ctttttcatt atcgagatca tcaccggttt gttcctgatg
     1201 ttgtattaca cgcccgcgcc cggcatttcc tacgacaata tgctgcgtat tctaagcaac
     1261 gtgccgctcg gccaatttac gcgcgacctg catcgtttag ccgctgaagg catggtcatc
     1321 gccgtgtggc tacatatggg ccgcacgttc ttgaccggtt cgtacaagaa gccccgccaa
     1381 ttcacttggg ccacgggtgt gatcttgttg ctcttgacga ttggtttgtc gtacacgggg
     1441 tatctgctgc cgtgggatca attggcttac tgggcagtga cgatcggcgc gtcgatggcc
     1501 gacgccgcgc cgccgccgga agtgggtaag gcggtgggct tgttgctcaa aggcgggccc
     1561 gatttaggcg ctggcggatt gctgcgcttc tatttgctgc atgtctttgc cttcccgctg
     1621 gtcgcgatca tctttgtctt tgtgcactat tacaaagtga tccgacatgg tcattcactg
     1681 ccgccggaaa atgaaaccat tggcgaagac aacgcgcgca aagttccgcc ggataagcgg
     1741 gtgccgttcc tgcccgatat ttttaccaac gaactgttgt ggttcgggtt tatcatggcg
     1801 gccatgatcg ttccgattgc gctcaacctc tatcacgcgc cattagagca cgcggcgaat
     1861 tcgcagagca cgccgttaca cacggtggcg ccgtggtact tcttgttctt gcaaggttgg
     1921 ttgaagttag gcgacaaaac tttctggggg gtgatcgtcc caacaatctt gatcggcctg
     1981 ttgatgttct ggccgtacat cgattacaac cccagccgcc gttatggcga tcgcaagatt
     2041 gcgttgatgg cgatgatgtt cgtgatcggc atcctcgtcg tctcgtctta tatgggcaca
     2101 cccggcttct cggttgttac tgcgccgcag ctcgaagtgg gcttccatgc cctgccgcct
     2161 gaacagcccg gccccatccg atcgctgccg ttcgatcaat tgccggtcgg cacgtggagc
     2221 acatatgacc tatcgggcat ccccgataat gcgaacgaac tgaaggcggt gatggccgac
     2281 atcagccacg aaattgatgc ggagccgaaa tcaatcgtca gtaatggcgt ggaagtgacg
     2341 taccagagca tgcatgccga attaaccatc gaagtcat
//
LOCUS       DASRTJ010000148         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_155013,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000148 DASRTJ010000000
VERSION     DASRTJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155013"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VFF70_03180"
     CDS             complement(<1..1183)
                     /locus_tag="VFF70_03180"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY43729.1"
                     /translation="MTTLDSVIVGAGQAGLGVSYFLQHAGRQHVVFERGRIGESWLSQ
                     RWDSFQLNTPNFMNVLPGLPYTGLETDGFWRRDELVHYFQGYVEHFQLPVRTGVNVVS
                     IERAEDEQRFIVKTKIDGQVEESVLSRSIVIASGSQQTRKFPPTRVRLPHTITQLHTA
                     DYRSAKTLPPGAIVIVGSGQSGCQIAEDLLAAGRTVYVCTSKVGRAPRRYRGRDLLEW
                     WIDMKHLDVTLASLKDKTVSRAAQPQISGLGRYGHTVSLQQLARQGAVILGRLLDSEE
                     STLILSDEAPAHVRYADEFSQQLKTGVDNYLAQAGITPPPLEDDPADVPDPEAKCVSP
                     LRQLNLDEAQVSTIIWATGFTGDFSWIHLPVLDAEAKPIHQRGISPVPGVYFIGFPWL
                     SS"
     gene            1485..3986
                     /locus_tag="VFF70_03185"
     CDS             1485..3986
                     /locus_tag="VFF70_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HZY43730.1"
                     /translation="MNRRPGSIVIALAVSLMLIAVLMVSIANAASPRPDGASLIRPLG
                     PSSSGAIRISQVYGGGGNASATYKNDFIELFNIGTTSVTMNSWSVQYAAAAGSAWSSN
                     ITVFTATLAPGQYYLIQEGSGGATGITLPITDAGGSTNLSASAGKVALVNNATALNGT
                     CPITNASVVDFIGYDTTANCSETANATGPSNNTTANIRKLKGCQDLDDNSKDFSLLSV
                     NPRNTSSPINICGLSISKSAVAQTNYNSAFTYTLTITNATITATDVVITDLLPSNVTY
                     VPNSASHAGDLLNGDTMSWTIATMTHTAIVSRTFVVTAPTTSGSIVNQLYRVSAGNIT
                     SDAFGSAITTTVLAPALGMIKTATPNSNVTYHSLVTYTLIVSNGGQADALNTFITDTL
                     PAEVDFAHWITSSGAVMNTDQITWTGTISIGKAITFTFVVTHVGAYADVVTNTAQFDQ
                     IGGSGAASATFSVESGTPSLSISKTASPNNNVTYHGTVTYTLIVSNQGTADALNTLVT
                     DTLPSEVDFAQWIDQPIGANPNSDQITWAGTISVGQPITFTFVVTHVGSYGDVVTNTA
                     QFDQASGSGSASAVFNVVPALPSLSLAKAVTTIHVPVKLGDPITYTIVVTNSGPGDAI
                     GVLITDVLPSGVGGANLNQTINISAGQRISFTLAAVVTNNVAYYGKSITNTVSFSHTS
                     GSGAASAVITIEAAPPANISAIKFSMPNNQRVLPNSLVTYTIVLSNSGGISATVRITD
                     VINSYTSVFSALNFTQAPSGTLKWSGVVTAGQSITLNFVARVNAIGQLAVGHTILNNA
                     ATIDDGVHAPFNVNAPDPPWLDVYGVYLPLVKKNS"
BASE COUNT          996 a         1086 c         1093 g          963 t
ORIGIN      
        1 ggctactgag ccacggaaat ccgatgaagt acacaccagg caccggcgaa attccccgtt
       61 gatgaatcgg cttagcctct gcgtcgagga cgggcaggtg tatccagctg aaatctccag
      121 tgaagcctgt ggcccagatg atggtgctga cctgtgcctc gtcaagattc aactgtcgca
      181 atggcgaaac gcacttggct tcagggtcgg gcacgtccgc tggatcatcc tcgagtggtg
      241 gcggtgtgat acccgcctgc gccagatagt tatcaacccc ggtctttaat tgttgcgaga
      301 attcatcagc atagcgtaca tgagcgggcg cctcatcgct caggatgaga gtgctttctt
      361 ctgaatccag caagcgaccc agaatcacag caccctggcg cgctaattgt tgcaggctga
      421 ctgtatgtcc ataacgtcca agacccgata tctgcggttg agctgctcgg ctaacagtct
      481 tgtcctttaa actagccaat gtgacatcga gatgcttcat gtcaatccac cactccaaca
      541 ggtcacgtcc tcgatagcgg cgaggagcac gtcccacttt gctcgtacat acatacaccg
      601 tccggccagc ggccagcaaa tcttcggcaa tctgacaacc ggattgaccg ctgccgacaa
      661 tgacaatcgc acccggcggc aatgtctttg cgctacggta gtcggctgta tgcagttgag
      721 taatagtgtg aggtagccgg acacgagtcg gaggaaattt ccgcgtttgt tggctaccag
      781 aagcgatgac gatggaacgg cttaacacag actcttctac ctgcccatcg atttttgttt
      841 tgacgatgaa gcgctgttcg tcttcggccc gctcgatcga gacgacattg acgccagttc
      901 gaaccggcaa ttggaagtgt tcaacgtatc cttgaaaata gtggaccagt tcatcgcgcc
      961 gccaaaagcc atcggtctct aggccggtgt agggcagccc gggcaagaca ttcatgaaat
     1021 tcggcgtatt gagttggaat gaatcccagc gttgggataa ccacgattca ccaatccgtc
     1081 cccgctcaaa gacaacatgc tggcggccag catgttgaag aaaataactg actcccagac
     1141 cggcttgacc agcaccaaca atcacactat ctagtgttgt catggcatta acttcctgag
     1201 tcgtcgatta atacaagccg ccccctatgg attaagtggt acgaattctg tataacatcc
     1261 ctgtggaggt gtgttagcca gaacgtgttc cggctaacgg ttaatatctc aaatcaaagt
     1321 atagatcgat tcgatctcaa gtcaagttgc ggtgatgtgc cgcgcccctt gcacgcgatt
     1381 gacgattagc gacttacatc ttcatgcgct tcattatata atccgcctat tccgttattc
     1441 ttcacaactg cacccgctgc ttgatcgcac acaggagatt gaacatgaat cgcagaccgg
     1501 gatcgatcgt gattgctctc gccgtgagtt tgatgcttat tgccgtgttg atggtgtcga
     1561 ttgcaaatgc cgcctctccg cgaccagatg gagcatcact cattcgaccg ttagggccgt
     1621 cgtccagcgg cgcgatccgc atcagtcaag tgtatggcgg gggcggcaac gcgagcgcga
     1681 cttacaaaaa tgattttatt gaattgttca atattggaac gacatcggtt acgatgaaca
     1741 gttggtcagt gcaatatgcg gccgcagctg gctctgcttg gtcttccaat atcacagtct
     1801 ttactgccac gcttgcgcca gggcagtatt atttgatcca agaagggtcc ggtggcgcga
     1861 caggcataac tcttccaatt acagatgcag gcggctcaac aaatttgagc gcatccgcag
     1921 gcaaagtcgc gctggtcaac aatgcgacag cattaaacgg gacatgtccc atcacaaatg
     1981 catcagtagt cgacttcatt gggtacgata caactgcaaa ttgtagtgaa actgcaaatg
     2041 caaccgggcc aagcaacaac acaacagcca atattcgcaa attgaagggc tgccaagatt
     2101 tagatgataa ctctaaagat ttttcattac tctcggtaaa tccccgcaac acctcatcgc
     2161 cgatcaacat ctgcggcttg tcgatcagca agtcggcggt ggcgcaaacc aattacaatt
     2221 cggcctttac ttacacgctg acgatcacca atgccacgat cacggcaacc gatgtggtca
     2281 tcaccgatct gctgccatcg aacgtgacgt acgtgccgaa cagcgccagt catgcaggcg
     2341 atctattgaa tggcgatacg atgagttgga caattgccac tatgacgcat acggcaatcg
     2401 tttcgcgcac gttcgtcgtg actgcgccga cgacgagcgg ctcgatcgtc aatcaactct
     2461 atcgcgtgtc ggcgggcaac atcacgtcag atgcttttgg aagtgcaatc accacaacag
     2521 tgctggcacc agcgttgggt atgatcaaga ccgccacgcc gaacagcaat gtgacttatc
     2581 acagtttggt gacttatacg ctgatcgtat cgaacggcgg tcaggcggat gcgctcaata
     2641 cttttatcac ggacaccctg cccgccgaag ttgatttcgc gcattggatc acgtcatcgg
     2701 gcgcggtgat gaatacggat caaatcacgt ggactggcac gatctcgatc ggcaaagcga
     2761 tcacgttcac gtttgtcgtc acgcatgtcg gcgcgtatgc agatgtggtg acgaacacgg
     2821 cgcagttcga tcagattggc ggaagcggcg cggccagcgc aacgttcagc gttgaatctg
     2881 gcacgccgag tttatcgatc agcaagaccg catcgccgaa taataatgtg acgtaccacg
     2941 gcactgtcac ttacacttta attgtgtcga atcaaggcac ggccgacgcg cttaacacgc
     3001 tagtcaccga tacgttgcca agcgaggttg atttcgcgca gtggatcgat caaccgatcg
     3061 gggctaaccc gaactccgat cagattacgt gggccggaac gatctcagtc ggtcagccga
     3121 tcacgtttac gtttgtggtc acgcatgtcg gtagctatgg cgatgtggtg acgaataccg
     3181 cgcagtttga tcaggcgagt ggttcaggtt cagcgagtgc agtgttcaat gtggtgcccg
     3241 ccttgccttc actctcgctt gctaaagcag tgacgacgat tcatgtgccg gtgaagttgg
     3301 gcgatccgat cacgtacacc atcgtcgtga cgaacagcgg gccgggcgat gcgatcggtg
     3361 tgttgatcac ggatgtgtta ccctccggtg taggcggcgc gaatctcaat caaacgatca
     3421 acatcagtgc gggtcaacgc atttcattta cccttgctgc agttgtgacg aacaacgtgg
     3481 cgtactatgg caaatcgatc acgaacacag tttcattcag ccatactagc ggcagtggcg
     3541 cggcctcggc ggtcatcacg atcgaggctg cgccccccgc aaatatcagc gcgatcaaat
     3601 tctcgatgcc gaacaatcag cgcgtcctgc cgaatagtct ggtcacgtac acgatcgtgt
     3661 tgagcaacag cggtggcatc agcgcgacgg ttcgcatcac ggatgtcata aattcttaca
     3721 cctcggtctt cagtgcgttg aacttcacac aagcgccgtc ggggacgttg aagtggagcg
     3781 gtgtggtcac ggcagggcaa tcgatcacgt tgaactttgt ggcgagagtg aatgcgattg
     3841 ggcaactagc ggttggacat acgatcctga ataatgcggc gacgatcgat gatggcgtgc
     3901 atgcaccatt caatgtgaac gcgcccgatc ccccgtggtt ggatgtgtat ggagtttatc
     3961 tgccgttggt gaagaagaat tcatagaaaa agtttgagca tcctgagcgg agatgtgaat
     4021 ctgtaagatt cacattgtag tcgaaggacg caggtctgga acaagacgcg tccttcggct
     4081 tcgaggtgaa gttgaaaact tcacctcttt gctcaggatg ctttcatttt atctgcca
//
LOCUS       DASRTJ010000149         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_311245,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000149 DASRTJ010000000
VERSION     DASRTJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311245"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFF70_03190"
     CDS             <1..1488
                     /locus_tag="VFF70_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43731.1"
                     /translation="ASGLIMSVLVVAIAGWWYWRNQQLYGDWFGLNMMVAIAGPRVPT
                     PTLAQLISEFDGFRYSYWALFGAVNIVTFPAAYVIFDAFSAAALIGWLVWQVRAWRRS
                     DRDQLTIMTLLAVYSLIVFVGVVSWTMLTPATQGRLLFPALTVISLSLWLGWETLWSI
                     IKARLSWTDRVLAAGQIIMPVFMLGTALIVPFRDIIPTYAGPNRLTVDQLPNDLVPVK
                     VDYGGAIRLIGYGLPTDQTASRTLDFTLYWQCLTQPTVDFSAFIIVYGRNQTEVGKRD
                     AYPFHGLFATSQCAPGTIFADPYRVPLAADADRPTVLRVQVGLKDWQHSIELTPSIAN
                     QQIPALIFIAGKLAPIAIDATSPYTPYARLGDFIELNSATIPTQVRSGESLPINLQWH
                     ALSAPPESYTVFVHLLDEQGHLAAQADGLPVMGDYPTNWWASGEIISDQHTLQLMPDM
                     PPGKYRIAIGLYRLADGIRLPVVDAAGNTQIDAQFILTTTLVVSH"
BASE COUNT          349 a          433 c          458 g          441 t
ORIGIN      
        1 gcatcgggct tgatcatgag tgttctggtt gtggctattg ccggttggtg gtattggcgc
       61 aatcagcaat tgtatggtga ctggtttggc ttaaacatga tggtggcgat cgccgggcca
      121 cgtgtaccca cgccaacgct agcacaattg atcagcgaat ttgatggctt ccgttattcg
      181 tattgggcgt tgttcggcgc cgtcaacatt gtcactttcc cggccgcata tgtcatcttt
      241 gatgcgttct cagcggcagc tttgatcggc tggttagttt ggcaagtgcg ggcttggcgg
      301 cgatcggatc gcgaccaatt aacgataatg accttactgg ctgtctacag cctgatcgta
      361 tttgtcggcg tggtcagttg gacaatgttg acaccggcca cgcaaggccg tttgctgttt
      421 ccggccttga ccgtaatctc actcagcttg tggctgggct gggaaaccct ctggtcgatt
      481 atcaaagcgc ggctgagctg gaccgatcgg gtcttagctg cagggcaaat tatcatgcca
      541 gtatttatgc tgggcacggc gttgattgtt ccattccggg atattatccc aacgtatgcc
      601 ggtccaaata gattgacagt cgatcaacta ccgaacgatc tggtccctgt gaaagttgac
      661 tacggtggtg caatccgcct catcggttat ggcttgccga cggatcaaac tgcgtccagg
      721 acgttggact tcacgcttta ttggcaatgt ctcactcagc cgaccgtaga cttcagcgcg
      781 ttcatcattg tgtatggacg caatcaaacc gaggtcggca agcgcgatgc gtatcccttt
      841 cacggtttgt tcgccacaag ccaatgtgca cccggaacga tctttgctga tccttatcgt
      901 gtgcctcttg ctgcagacgc cgatcgaccg actgtgctgc gggtgcaagt cggacttaag
      961 gattggcagc actccatcga gctgacaccc tcgatcgcta atcagcagat tcccgcattg
     1021 atctttattg ccggcaaact tgctccaatc gccatcgatg caacttcacc ctacacgcct
     1081 tacgcgcgct tgggtgattt catcgaattg aactctgcta cgatcccaac tcaagttcga
     1141 tccggcgaga gcttgcccat caatttgcaa tggcatgcac tctcggcgcc gcccgagtcc
     1201 tacaccgttt ttgtgcattt actcgatgaa caaggacacc tcgcggcgca agccgacggc
     1261 ttgccggtca tgggtgatta tccaacgaac tggtgggcga gcggcgaaat aatcagcgat
     1321 caacatactc tgcagttgat gcctgatatg ccgccgggaa agtatcgcat cgcaatcgga
     1381 ctgtatcgat tagcggatgg tatacgcttg ccggtcgttg atgcggcggg caatactcag
     1441 atcgatgcgc agtttatctt gacgacgaca cttgtggtct cacactaatt tcatcggcgt
     1501 aagtagttcg ggtatgaaag aattgggctg caccggcgat actgatcgca tgaagtaagt
     1561 gtttgagtct tgcgcatgat cgatcggctg gccttaacgg tctgagagct gcttcagttg
     1621 agcaggcgat acgcgcacac cgcctatatt tgggctgagg aaaatggtct gtgtgctata
     1681 c
//
LOCUS       DASRTJ010000150         6656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_344316,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000150 DASRTJ010000000
VERSION     DASRTJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344316"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFF70_03195"
     CDS             complement(<1..457)
                     /locus_tag="VFF70_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZY43732.1"
                     /translation="MIRALITDFGGVLVRTRTDRSRRELERKLGWPLHTLEDRVFNSD
                     VSIRASHGEITEDAFWDQAARDFNLAQYQLTGAEFRHEFFVDDFLDEELVQFMRGLRP
                     AIKIGLISNAWLGLRSVLQTVFHIDDMFDEIVISAEENVMKPDARIYRLA"
     gene            complement(506..1456)
                     /locus_tag="VFF70_03200"
     CDS             complement(506..1456)
                     /locus_tag="VFF70_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZY43733.1"
                     /translation="MLSFLDRLAQNKPILLDGATGTELNRRNIDTSLPLWSARALIEY
                     PDAVRSIHADYILAGADMITTNTFRTHRRTLDRAGQFNTGPTAWRSAGRARGLTQLAV
                     QLAREAISRSGRSIFIAGSMSPLEDCYSPQLVPPDDELRVEHQEMARDLADAGCDVLL
                     IETMNTIREAVIATEAALATGLPVGVSFVVGPGGRPPDDPNALHNHEAMTLLSGESID
                     DAVIAVSYLKPNVILINCVPFNVIDRAFDLLRAASDRPIGLYANVGHTDAEVGWALTD
                     DVEPEAYARRARDWIDRGAKIVGGCCGTTPKHIAAIKKIM"
     gene            complement(1470..5765)
                     /locus_tag="VFF70_03205"
     CDS             complement(1470..5765)
                     /locus_tag="VFF70_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZY43734.1"
                     /translation="MQRIQKSFIRLIAVVVLAAFGLWAIRPNTVSSQPQPISRPSPTG
                     LSASTSISITPALAIKFHPTLLKQVLTAESQQPDTQFRVIIHVQPVTGMNAAASLPAS
                     ASIVDRRRAMVNNLQSIADRSQAGVRALLDQAQLTDRAHDVRLLWINNSVAARVDRSI
                     LIALAAREDVTRIDLDEYRKWIDAELASIPQSAVHTPQSVEWNIAKIRADQVWAALGI
                     SGTGVVVANMDTGVDWQHPALLASYRGYNPKGLSNHLYSWFDATPDADQYPYDGYGHG
                     THTMGTLVGSGGIGVAPGAKWIAARVLDNTGSGYDSWIHAGFQWIMAPGGDPSQAPNV
                     LSNSWGNSNGYDQSFRSDVIALNAAGIATFFSNGNSGPANGSVGSPASYPEAFGIGAT
                     DANDLIALFSSRGPSPFGPIKPDVSAPGVNVRSSYPGGVYRSFSGTSMAAPHVAGVAA
                     LMLSAMPSLTITSTRYILTSTAIRPITSVVYPNNDYGWGRIDALKAVLAVAHTGLITG
                     SITRSDNSAPISIATIQAQSMVNTSGVASTDALGQYALNLLPSTYTLTASAFGYVSQT
                     VSGVNVITNAVTRHDFSLPPLPTGTVQGRVTDLTGTHLLSGSIGIESEPINVNFSGTY
                     SIALPGGVYVIRAQSPLHRIVTATIMITAGDTITQNFFLPDAPRILVVDSGAWYNASE
                     VDYYRQALDDNNYSYTVWPIRDPNKDVPTTSTLRQYTAVLWSSPLDSPGYIGASYAVG
                     DYLKQGGNLLLSGQDVGFWDGYWIYSPYFPSQMLAQYVADDGPTRQLTGQQLFAGQVI
                     SISGSGGADNQLYPDVIASLDPLLTRGAFDYLPDQSGGQSIGLCQPQRAVYLAYGFEA
                     INDRNARAEVMSRTFAYFNSPRVRFNYALDQITDPVIGSAGSVVSASIDLHNLDEVAP
                     TTTFSISVSSAWPASITPTLTTINTCDQREITLTVHIPITASIDTRQMVTITAHPVIS
                     PVLSVSSTLIVKAPASVLLVDDDRWYPVDLSYRDALNANNISYDLWRVPTSWAGIDPA
                     VPSLDRLSWYPEVIWFTGYDWYQTLTNYDEQVLSQYLAQGGRVLLSSQGYLAEHGLTN
                     FGRSALGILDFIDNVSTTTARGPYGSLFDGLDFSALNFPYPNFTVALAPQPNAQVALI
                     GSHGWPIALLHRSNLGKSMFMAFGFEDLPLAMQPEALNRSIGYLSWLGDSQVKFDRSM
                     AASGAVITATIVTLNDMPVTITQAAYTITLPLSATLKNGNPLIWAGSLLPNQSITATV
                     VITLATDLNAGDVVTLPVQFVDQDHALQFVSTARLAIDAPAFAFDLQPSSASIRPHDL
                     ITWTLTARNFGLDAPVAVITDLLPFNQTIVSGSLAYDSGSASYLSGTIRWNGSISAGQ
                     SITLSYQMSGPLVFSDHVYYAGAVMSDGSDVWQAGNWLTVQPYQSYLPIVRKG"
     gene            5898..6602
                     /locus_tag="VFF70_03210"
     CDS             5898..6602
                     /locus_tag="VFF70_03210"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HZY43735.1"
                     /translation="MKRFATISMFISALLLSACAPTLPDLPPNDIVTRSGQALLQVPS
                     MHFKIDLSGSSVMINQATLLSLRSVEGDFARPDRMGVHLKVILAIAAAEMDMIALGND
                     QYVTDVLTHQWEVLPPEFGFNPAVLFDPQNGLEQTLKAGLDNTQSLGIEAIDGVATYR
                     VKGSLDGTRVQIMSGGLLSTGQIDAELWVEGSTFLPRKATLTDRGRDPKNPTVWSMSF
                     SSFSKTVTITAPTIGK"
BASE COUNT         1615 a         1864 c         1784 g         1393 t
ORIGIN      
        1 gcgcaaggcg atagatgcgc gcgtcgggct tcatcacatt ttcttcagcg gagatgacga
       61 tctcatcaaa catgtcgtca atgtgaaaca ccgtctgcaa tacggaccgc aaacccagcc
      121 aggcattgct gatcaagcca attttgatcg cgggtcgcag gccgcgcatg aattggacta
      181 actcttcatc taaaaagtcg tccacaaaaa attcatggcg gaattcagcg ccggtcaatt
      241 gatattgcgc caaattaaaa tcgcgggcgg cttgatccca gaaagcatct tccgtaattt
      301 cgccgtgcga ggcgcggatc gatacatcgc tgttgaagac acgatcctca agggtatgca
      361 gcggccaacc taacttgcgc tctaactcgc gccgcgatcg atcagtgcgc gtgcgcacca
      421 gcacgccgcc aaaatcagta atcaatgctc gaatcatttt gctcctaaat aaaatttcta
      481 agtgcagggc gaggtaacct cgcttctaca ttattttctt gatcgccgca atatgcttcg
      541 gcgttgtgcc gcagcagccg ccgacgatct tcgcgccgcg atcgatccaa tcgcgggcgc
      601 ggcgggcata cgcctctggc tcaacatcat cggtcaacgc ccaacccact tcggcatccg
      661 tgtggccgac gttcgcatac aaaccgatcg ggcgatcact ggccgcgcgc aagagatcga
      721 aagcgcgatc gatcacattg aacggcacgc agttgatcaa gatcacattt ggcttcaaat
      781 agcttactgc aatgactgca tcatcgatcg attcgccgct cagcagcgtc atcgcttcat
      841 gattgtgtag cgcgttcgga tcatctggcg gacggccacc cggcccgacg acaaaactca
      901 cacccactgg caagccggta gcgagagccg cttcggtcgc aatcaccgcc tcgcggatcg
      961 tgttcatcgt ttcgatcaac agcacatcgc aaccggcatc cgccagatcg cgcgccatct
     1021 cttgatgctc aacacgcagt tcatcatccg gcggaaccag ctgcggcgaa taacaatctt
     1081 ccaatggcga catcgatccg gcgatgaaga tcgatcggcc agatcgactg attgcttccc
     1141 gcgccagctg caccgcgagt tgggttagac cccgcgcccg acccgcactg cgccacgcag
     1201 tggggccagt gttgaactgc ccggcccgat cgagtgtgcg gcgatgcgtg cgaaatgtat
     1261 tcgtcgtgat catgtccgcg ccagccaaaa tgtaatcggc atggatcgat cgaacggcat
     1321 cgggatattc aatcaacgcg cgcgccgacc acagcggcaa actcgtgtcg atgttgcgcc
     1381 gatttaattc ggtacccgtc gcgccgtcca gtaagatggg tttgttttga gcaagtcgat
     1441 cgagaaatga aagcatgtcg tgttgtatcc taccccttac gaacgatcgg cagataagat
     1501 tgatacggtt gcacggtcaa ccaattgccc gcttgccaca catccgagcc gtctgacatg
     1561 acagcgccag catagtaaac atgatctgaa aaaactagcg gcccgctcat ttgataactc
     1621 aacgtgatcg actgccccgc cgagatcgat ccgttccagc gaatggtgcc gctcagataa
     1681 gaagccgagc ctgaatcata cgccaatgat ccactgacga tcgtttgatt gaaaggtagc
     1741 aaatcggtga tcaccgcaac cggtgcatcg agtccaaaat tacgcgcggt caacgtccac
     1801 gtgataagat cgtgcggacg aatcgatgcg cttgacggct gaagatcgaa cgcaaaggcc
     1861 ggcgcatcga ttgctaagcg cgccgtcgag acaaactgaa gcgcgtggtc ttgatcgaca
     1921 aattggacgg gcagagtgac cacgtcgccc gcgttgagat cagtagcaag tgtgataacc
     1981 accgtggctg tgatcgattg attgggcaac agcgatccag cccaaatcaa tggattaccg
     2041 ttcttcaagg ttgcgctgag cggcaatgta atcgtataag cagcctgtgt gatggtaact
     2101 ggcatgtcgt tcagcgtaac gatcgtcgcc gtgataaccg cgcccgatgc ggccatcgat
     2161 cgatcgaact tcacttgcga atcacccagc cacgacagat aaccgatgct gcgattcaac
     2221 gcttcaggct gcatggcaag cggcaggtct tcaaacccga aggccatgaa catggacttg
     2281 ccaagattcg atcgatgcaa cagtgcaatc ggccagccgt gcgatccaat caacgccact
     2341 tgagcattgg gctgtggcgc caaggcaacc gtaaaatttg gatatgggaa gttcaatgca
     2401 ctgaagtcca agccgtcgaa taaactgccg tacggtccac gggcagttgt cgtcgacaca
     2461 ttgtcaatga aatccaatat gcccaaagca ctgcggccaa aattcgtcag gccgtgttca
     2521 gccagatagc cttgcgacga caacaacact cggccgccct gcgcaagata ttgcgacaaa
     2581 acttgttcgt catagttcgt cagggtttga taccaatcat agccggtgaa ccagatcact
     2641 tcgggatacc agctcaatcg atcgagtgac ggaacggccg gatcaatacc cgcccaactt
     2701 gtcggcacgc gccataaatc gtaagagatg ttattggcat tcaacgcgtc acgataagac
     2761 agatcgaccg gataccagcg atcgtcatcg accaggagca cactcgcagg cgctttgacg
     2821 atcaaggtgg acgaaacgct caacacaggt gaaatgaccg gatgagcggt gatcgtcacc
     2881 atctgccgcg tatcgattga agcggtaata ggaatatgga cagtcaaagt gatctcgcgc
     2941 tgatcacaag tgttgatcgt tgtgagtgtc ggcgtgatcg aggccggcca cgcgctgctg
     3001 acgctgatcg aaaacgtcgt cgtaggcgca acttcatcaa gattgtgcag atcgatcgaa
     3061 gcgctgacca cgctgccggc tgaaccaatt accggatcag taatttgatc gagcgcatag
     3121 ttgaagcgca cacgcgggct gttaaagtaa gcgaaggtgc gggacatgac ttcagcgcgc
     3181 gcattacgat cgttaatcgc ttcaaagccg tatgccaaat acaccgcacg ctgtggctgg
     3241 cacaaaccga tcgattggcc gccggactga tcaggcagat aatcaaaagc gccgcgcgtc
     3301 agcagtggat caagcgaagc gatgacatct ggatacagtt gattatccgc accccctgag
     3361 ccgctaatcg agataacctg acccgcaaat aattgttggc cggtcaattg ccgagtgggg
     3421 ccgtcatcgg caacatattg cgctagcatt tgacttggaa aatatggcga gtaaatccaa
     3481 tagccatccc aaaaaccgac gtcctgtccg ctcaacaaca ggttgccgcc ttgtttcaaa
     3541 taatcaccga cggcgtacga cgcgccgata taaccgggcg agtcaagtgg cgacgaccac
     3601 agcacggctg tatattgccg cagtgtcgaa gtcgtcggca catccttgtt cggatcacga
     3661 atcggccaga ccgtgtaaga ataattgtta tcatccaaag cttgtcgata ataatcgacc
     3721 tcactagcgt tgtaccaagc gccgctgtcc acgactaaga tgcgcggcgc atctggcagg
     3781 aaaaagttct gcgtgatcgt atcacccgcc gttatcataa tcgtcgccgt gacaatgcga
     3841 tggagcggcg attgcgctcg aatcacgtag acgccgcccg gcagtgcgat cgagtatgtc
     3901 ccgctgaaat tcacattgat cggttcagat tcaatcccga tcgatccact cagcaggtgc
     3961 gttccggtga gatcggtcac gcggccttgc acagtgccgg tgggtagcgg tgggagtgag
     4021 aagtcgtggc gtgtcactgc attcgttatg acattcacgc cactcactgt ttgcgagaca
     4081 tacccaaaag ccgaagccgt cagcgtataa gtggacggca acagattcaa agcatactgc
     4141 cccaacgcat cggtcgaagc cacaccgctg gtattgacca tcgattgagc ttgaatagtc
     4201 gcgatactaa tcggtgcgct gttatcactg cgtgtgatac tgccggtgat gagaccggta
     4261 tgcgcgacgg ccagaacggc cttcaaggca tcgatacgcc cccaaccata atcgttgttc
     4321 ggataaacga ctgaagtgat cggccggatc gccgtgctgg tcaaaatgta gcgcgtcgat
     4381 gtaatcgtca aactcggcat ggccgagagc attaaggcag ctactccggc gacgtgcggc
     4441 gcggccatcg atgtgccgct gaagctgcga tacacgccgc ccggataact cgagcgcaca
     4501 ttcacgccag gtgcagacac atcgggtttg atcggtccga aaggtgacgg cccgcgactg
     4561 gagaagagcg caattaagtc attcgcgtcc gtcgcgccga tgccgaacgc ctcgggatag
     4621 gacgccggtg atccgactga tccattggcc ggcccgctgt ttccattact aaaaaatgtg
     4681 gcgatgccgg cggcgttcaa cgcgatcaca tcgctacgga aagattgatc atagccgttc
     4741 gaattgcccc atgaattgct taatacattt ggcgcttgag acggatcgcc gccgggagcc
     4801 atgatccatt gaaagccagc atgaatccaa ctgtcgtagc cgctgccagt gttgtccagt
     4861 acccgcgccg cgatccattt ggcaccgggc gcaacaccga taccgccgct accgaccagc
     4921 gtgcccatgg tatgcgtgcc atgcccataa ccatcataag gatattgatc cgcatccggt
     4981 gtagcatcga accaactgta gagatgattc gacaaacctt tcggattgta gccgcgatac
     5041 gaagcgagca acgccggatg ctgccaatcg acgccggtgt ccatattcgc gacaaccacg
     5101 ccagtgccgc tgatgcccaa cgccgcccac acttgatcgg cgcgaatttt ggcaatgttc
     5161 cactccacgg attgcggagt gtggactgcg gattgcggaa tcgatgctag ctcagcatcg
     5221 atccattttc gatattcatc cagatcgatc cgcgtcacat cctcgcgggc agccagcgcg
     5281 atcaagatcg atcgatcgac gcgcgcggct acgctgttgt tgatccacaa caatcggacg
     5341 tcgtgcgccc gatcggttag ctgcgcctga tcgagcagtg cgcgcacccc ggcttgcgat
     5401 cgatccgcga tcgattgcag attgttgacc atcgctcgcc gtcgatcgac gatcgacgcg
     5461 ctggccggca ggctcgcggc cgcgttcatg ccggtaaccg gctgcacatg aatgatgact
     5521 cgaaactgtg tgtccggttg ttggctttcg gcagtcaata cttgcttcaa caacgtcgga
     5581 tgaaatttga tcgcgagggc aggcgtaatt gaaatggatg tagacgcaga taaaccggtg
     5641 ggactaggac gactgatcgg ttggggttga ctgctgacag tgttcgggcg gatcgcccac
     5701 aagccaaacg cggccaagac cacgaccgcg atcaaacgga tgaaagattt ttgaattcgc
     5761 tgcatgagac tgattatatc ccattgcctt tgtttgacac tggtacgtgg gtagcataca
     5821 atagacgcaa gtgaaatgtg attagtgatt tgtcgatact aattactgcc tactcatcag
     5881 cccgcatgag gccaagcatg aaacgttttg caaccatcag catgtttata agcgccttac
     5941 ttctcagcgc ctgcgccccc acgctgcccg atctaccgcc aaatgacatc gtgactcgat
     6001 cgggccaagc gctgttgcaa gttccctcga tgcatttcaa gatcgatctc agcggctcgt
     6061 cggtcatgat caatcaagcg acgctgttaa gtctgcgcag cgtcgaagga gatttcgcgc
     6121 ggcccgaccg aatgggcgta catctcaagg tcatcctggc tatcgccgct gccgagatgg
     6181 acatgattgc cctgggcaac gatcaatatg tcaccgatgt tttaacccat caatgggaag
     6241 tgctgccgcc ggaatttggt ttcaatcccg ccgtcttgtt cgatccgcag aatggcttgg
     6301 agcaaacatt gaaagcgggc ttggataaca ctcaatcgct gggcattgaa gcgatcgatg
     6361 gtgtagcgac gtatcgcgtc aaaggcagcc tcgacggcac gcgcgtgcaa atcatgagcg
     6421 gcggcttgct gagcaccggc cagatcgatg ccgagctgtg ggtggaaggc agcacgtttt
     6481 taccgcgcaa ggcgaccctg accgatcgcg gccgcgatcc caaaaacccg acggtctggt
     6541 cgatgtcgtt ttcatcattc agcaagaccg tcaccatcac tgcgccgacg atcgggaaat
     6601 gatctgtcat ttcgagcaca gcgagaaatc tctacgatga ttaaagtttg gcttaa
//
LOCUS       DASRTJ010000151         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_235681,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000151 DASRTJ010000000
VERSION     DASRTJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235681"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFF70_03215"
     CDS             <1..840
                     /locus_tag="VFF70_03215"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZY43736.1"
                     /translation="PQDLGAIWVGSESHPYAVKPTSTIVAEAIGAVPFTQAADWQFAC
                     KAGSEALQASLGFVGSGMAKYAMSIGMDTAQGRPGDALEYTAGAGGAAFIVGDAEESV
                     AIAEGSLSYVTDTPDFWRRPYAHYPEHASRFTGEPAYFKHITESAEHLMAEMGTQPDD
                     YTYAVFHQPNVKFPQRVAQMIGFKPDQIKTGLLVNDIGNTYAGSCMIGLTAILDQAKP
                     GDRVMMISFGSGAGSDAFSWRVTDRITERQSLAPKTREYVLRRSVIDYATYTRYRKKL
                     TMV"
     gene            907..2064
                     /locus_tag="VFF70_03220"
     CDS             907..2064
                     /locus_tag="VFF70_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZY43737.1"
                     /translation="MRDVSIIGIGQTPVGEHWDIGLRHLALEAVRAAANDAHIDHADA
                     LFVGNMAAGLIGGQSNLGALIADTVGLRGIEAVTVEASSASGAAALRQAYLAVASGAI
                     EFAIVLGVEKLSDVTGSASISAQLTATDSDYEAAHGVTPLAAAAMLMRRYMYEYDHVL
                     ADFAAFSINAHLNAATNPNAMFHTKVTAQSFVKAPMVADPINLFDVAPAADGAAAIVI
                     ASTEWAKQHGDGVVRIAGSAVSTDALAVHDRHDHLWLTAAELSANKAYKQANLSPKDI
                     QLFELHDSTTILAAMSLEAAGFAERGRGVSFAAHGGIARGGQIPISTLGGLKGRGDPG
                     GATGLYQIVEVVQQLREQAGPNQIANARIGMAQNLGATGATAVTHILVHEE"
     gene            2159..2557
                     /locus_tag="VFF70_03225"
     CDS             2159..2557
                     /locus_tag="VFF70_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZY43738.1"
                     /translation="MDIPRHWRLKSQRYALIGEVCGHCQSKLFPPRDVCPECGTPAKT
                     PFTFSGRGEVFSFSTVYQAPASHEEQAPYTVALIKLAEGPMLTAQLTDVDRDQIKIGM
                     PVEMATRVLQSNGDRGMIVYGYKFRPVGLN"
BASE COUNT          653 a          756 c          827 g          637 t
ORIGIN      
        1 ccccaagact tgggcgcgat ctgggttggc agcgagagcc atccgtatgc cgtcaagccg
       61 acctcgacga ttgtcgctga agcgatcggc gcagtgccgt tcacgcaagc tgctgactgg
      121 caattcgctt gcaaagccgg atcggaagca ctgcaagcct cactcggctt tgtcggatcg
      181 ggcatggcga aatatgcgat gagcatcggc atggacacgg ctcaaggtcg accgggtgat
      241 gcgctcgaat ataccgcggg tgcaggtggt gcggctttca tcgttggtga cgcggaagaa
      301 tccgtcgcga ttgctgaagg ctcgctatcg tatgtgaccg acacgcctga cttttggcgt
      361 cggccgtacg cgcattatcc tgaacacgcc agtcgattca cgggtgagcc tgcgtatttc
      421 aaacacatca ctgaatctgc cgagcacttg atggccgaga tgggtacaca gccggatgat
      481 tacacttatg cagtatttca tcagccgaat gtgaaattcc cgcagcgtgt tgcgcagatg
      541 atcggcttca aacccgatca gatcaagact ggcttactgg tcaacgacat cggcaacacg
      601 tatgctggtt cgtgcatgat cggcttgacg gcaattctcg atcaggcgaa gccgggcgat
      661 cgggtgatga tgatctcgtt tggatcgggc gcgggcagcg atgcgttcag ctggcgcgtg
      721 accgatcgga ttaccgagcg acaaagttta gcgcccaaga cgcgcgaata tgtattgcgt
      781 cgctccgtga tcgattacgc gacatacacc cgctatcgca agaagttgac gatggtctaa
      841 gccctttact aattactatt cactcatcat gaaaatagca attagtgatt agtggttgaa
      901 aacattatgc gtgatgtaag tattatcgga attggacaaa ctcccgtcgg cgagcattgg
      961 gacattggct tgcggcattt ggcgttggaa gccgtgcgcg ccgccgcgaa tgacgcccac
     1021 atcgatcacg ccgatgcgct gtttgttggc aacatggccg caggcttgat cggcggacag
     1081 agcaacctcg gcgcattgat cgccgacacc gttggactgc gcggcattga agccgtaacg
     1141 gtcgaggctt catcggcttc gggtgcggcc gcgctgcgac aggcttatct cgccgtggcc
     1201 agcggtgcga tcgagtttgc gattgtgttg ggtgtcgaaa aattatcgga tgtgaccggc
     1261 tctgcatcca tatcggcaca actgaccgcc acagacagtg attacgaagc ggcgcatggt
     1321 gtaaccccgc tggctgcggc ggcgatgttg atgcgccgtt acatgtacga gtacgatcat
     1381 gtgctggccg actttgcggc cttcagcatc aatgcgcact tgaacgcggc gacgaatccg
     1441 aatgcaatgt tccataccaa agtaaccgca caatcatttg tcaaagcgcc gatggtcgcc
     1501 gatcccatca acctatttga tgtggcgccg gcggcggatg gcgcagcagc gatcgtgatc
     1561 gcttcgaccg agtgggccaa gcagcacggc gatggcgtgg tgcgcattgc aggctcggcc
     1621 gtttcgactg atgcgttggc ggttcacgat cgacacgatc acttgtggtt gacggcggcg
     1681 gaactatcag cgaacaaagc ttataagcaa gctaatcttt cgcccaaaga cattcaactc
     1741 tttgagctgc acgattcgac gacgattctg gccgcgatgt cacttgaagc cgcgggcttt
     1801 gctgagcgcg gtcgaggtgt cagttttgcg gcgcatggcg gcatcgcgcg cggcggccag
     1861 attccgatca gcaccctggg cggcttgaaa ggccggggcg atccgggtgg cgcgacgggc
     1921 ctctatcaaa tcgtcgaagt cgttcagcag ctgcgcgagc aagccggacc caatcaaatt
     1981 gcaaatgcgc gcatcggcat ggcgcagaac ttaggcgcga ccggcgcgac agcggtgaca
     2041 cacattttgg tgcacgaaga ataatgagaa ctgagtgaca agcaatttac tcatgatcaa
     2101 ttcgcaaaac taaacccgcc tcactgggtt atctatttag gaagcatgga gagataaaat
     2161 ggacattcca cgacattgga gattaaagag tcagcgctat gcgctcattg gcgaagtgtg
     2221 cggacattgc cagagcaaac ttttcccgcc gcgcgatgtg tgcccggagt gcggcacacc
     2281 ggcgaagacg ccgttcactt tttcaggccg cggcgaagtg ttctctttca gtacggtcta
     2341 tcaagcgcca gccagccatg aagaacaagc gccttacacc gtggccttga tcaaattagc
     2401 ggaaggcccg atgcttaccg cacagctgac tgatgtcgat cgcgatcaga tcaagatcgg
     2461 catgccggtg gagatggcga cgcgtgtgct gcagagcaac ggcgatcggg gcatgatcgt
     2521 atacggctac aaatttaggc ccgtcggttt gaactgaacg atcggtatct gagaacagaa
     2581 gtgatagttc gttttgatcg atgagttgat tcgatcaaga tcaatctccc ttaaaaatca
     2641 atatcccgat gagctgctcc ggttcatcgg gattttttcg tatttcatga tagacaggca
     2701 caaccgatcg ggagtataat ccatgtaact tttagccgcg gggggcagca tgtttccatt
     2761 tctcgatcgc tccggcaata tctcacactc acatcgcacg gctgtcttaa tcctcaaacc
     2821 taagataaaa attccacaca gcctgctcat cacgctgatg ctggcgatct ttc
//
LOCUS       DASRTJ010000152         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_46635,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000152 DASRTJ010000000
VERSION     DASRTJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46635"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFF70_03230"
     CDS             complement(<1..1302)
                     /locus_tag="VFF70_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43739.1"
                     /translation="MAPEVEWDVDTSTGSQTIVKTPPPRSPSPRNRIIVLLVVVFGVT
                     LGVIYRSIPEPPKPPTPTIRPTPTIAISIDRVIDQEARALERGDPQVFMRLQDPVDSQ
                     WRTQQINSFKKWGEPPDTSDLYTIINSDALGSDRRWVDISQARDGQFFRETRFYRLYD
                     SKWVRTAPDYSLWGDGRLLRTNHFDLLYQEGDDYHAQIVAQQLEKFYTRVCQDLNCTL
                     GKPIGSLVFRVCRALDCSDGAGVDPNPVPQFTVVMSPTIKATTAGGFSTQHITITMPS
                     PRLSGLYFQSLYDSAPGNDEPLVQMAIDKLYVPHLVAKIASGRPALLSDFKKGDLFVG
                     NITQWEINRIIPISAFSDAQIFLTLQNVKDQDLLSLDVLWDAIPPYDLPQSQLDMLVT
                     ETWSVIRFIETKYGSESVVHFLNAIGPAQSMSDALQNGLNVS"
     gene            complement(1413..>2116)
                     /locus_tag="VFF70_03235"
     CDS             complement(1413..>2116)
                     /locus_tag="VFF70_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43740.1"
                     /translation="DVYERLCGDLNCPQQRQCIEGLIDNLVGCSPFPRTLTITLQLLP
                     AAEQSDWVIYRGAEFISLPSPRITGLYKRWWSENDPIQRTAYDSLIFPIAHLASGDPN
                     RWTDDLGGNWYLEAIAGWELHRVEFIPADVRLDQAQQLYSSLLADVQLIPLDTVWAWH
                     IDQDPSMGADNDRGQIEAEAVIAYIEQQYGSTGLVKFLNALGPASALEPALQASLGIS
                     YLEFNQRWLAWLNKE"
BASE COUNT          490 a          547 c          573 g          506 t
ORIGIN      
        1 gctgacattc aatccattct gaagtgcatc cgacatcgat tgcgctgggc cgatcgcgtt
       61 gaggaaatgc acgacgctct ctgagccgta ttttgtttcg atgaatcgaa tgactgacca
      121 agtttcagtg actaacatat cgagctgtga ttgtggtaga tcataaggcg gtatcgcatc
      181 ccacaacacg tcgagtgaca gcaaatcttg atctttgacg ttttgcagtg ttaaaaagat
      241 ctgtgcatcg ctgaacgctg aaattggtat gatgcgattg atctcccact gcgtaatgtt
      301 gccaacaaag agatcacctt tcttgaaatc ggacaagagt gctggcctgc ccgatgcaat
      361 tttggcgact aggtgcggca catataactt atcaatcgcc atctgcacga ggggttcatc
      421 gtttcccggc gcgctatcgt agagcgattg gaaatataaa ccgctcaagc gcggtgatgg
      481 catggtgatc gtaatgtgtt gtgtactaaa accgcctgct gtagttgctt taattgtcgg
      541 cgacatgacc actgtaaact gtggcactgg attgggatcg acacccgctc catcgctgca
      601 atctaaagcc ctgcatacgc ggaagaccaa ggagccaatc ggcttgccca aggtgcagtt
      661 caaatcctgg cagacgcgag tgtaaaactt ttccagttgc tgcgcgacaa tctgcgcgtg
      721 gtagtcatcg ccctcttgat agagcagatc gaaatgattg gtgcgcagca agcgcccgtc
      781 gccccacaaa gaataatcgg gtgcggtgcg gacccatttg ctatcgtaca atcgatagaa
      841 gcgtgtctcg cggaaaaatt gcccgtcgcg cgcttgacta atatcgaccc acctgcgatc
      901 gctgcccagc gcgtcgctgt tgatgatcgt atacaagtcg ctggtgtcgg gcggttcgcc
      961 ccacttctta aatgaattga tctgctgagt tcgccattgt gaatcaacgg gatcttgcaa
     1021 gcgcatgaag acttgaggat cgccgcgttc aagcgcacgc gcttcttgat cgattacgcg
     1081 atcgatcgat atagcgatgg tgggcgtagg acgtattgtc ggtgtaggtg gcttgggcgg
     1141 ttcggggatc gatcggtaga tcacgcccag cgtgacgccg aagacgacga cgagtaagac
     1201 gataatccgg ttgcgcggcg atggcgatcg aggcggcgga gttttgacaa tcgtctgact
     1261 gccggttgaa gtatcaacat cccattcaac ttcaggcgcc atgttagttc actcacgttc
     1321 gcacccccgc gtgtgtctcg atcaaattat atctgtttta tgaatggtgc gatagccaga
     1381 gggtggaatg gcgagaaaaa atttgccagt tctcattctt tattgagcca cgccagccag
     1441 cgttgattga attccagata actgatccct aatgaagctt gcagcgccgg ctcaagcgcg
     1501 gaggccgggc cgagggcatt caagaattta acgaggccgg tcgatccata ctgctgttcg
     1561 atgtaagcga tcaccgcttc ggcttcgatt tgtccccgat cgttgtcggc gcccatgctg
     1621 ggatcttgat cgatgtgcca cgcccagacg gtgtccagcg ggatcaactg cacatcggct
     1681 aggagactgg aatatagctg ctgcgcttga tccaaacgca catctgccgg aatgaattcc
     1741 acgcgatgca gttcccaacc ggcgatcgct tccagatacc aattgccgcc cagatcgtct
     1801 gtccagcgat ttggatcccc ggaggccaga tgcgcgatcg ggaaaatcaa actatcgtaa
     1861 gctgtgcgtt gaatcggatc attctcagac caccaacgct tgtacagtcc ggtgatgcgc
     1921 ggcgaaggca ggctgataaa ttccgcccct cgatagatca cccaatccga ttgctctgcg
     1981 gcgggcagca gctgcagcgt gattgtcagc gtgcgcggga aagggctgca acccaccagg
     2041 ttatcgatca agccctcgat gcattgccgc tgttgcggac aatttagatc gccgcacaat
     2101 ctttcataca catctt
//
LOCUS       DASRTJ010000153         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_311734,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000153 DASRTJ010000000
VERSION     DASRTJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311734"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFF70_03240"
     CDS             complement(<1..623)
                     /locus_tag="VFF70_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HZY43741.1"
                     /translation="MSFYDPLSKDFPFTVGIEEEYQIIDPVTRDLRSYVTQILEEGQL
                     ILREQLKPEMMQSVVETGTHVCRTIAEARAEIVRLRGTIAGLAKKNGLTIAAAGTHPF
                     AAWADQEVYPHERYYGVLNEMQDAARQLLIFGMHVHVGMPDLETGIQIQNVARYFLPH
                     LLMLSTSSPFWMARNTGYKSYRSIIFSNFPRTGFPGQFVSYAEFQSYV"
     gene            complement(663..2021)
                     /locus_tag="VFF70_03245"
     CDS             complement(663..2021)
                     /locus_tag="VFF70_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43742.1"
                     /translation="MTLKQIIDEYHSLLDDDSARANNDVLVAASKREGLFFGERPICI
                     VLRPRLLTVDQYAHLQEVCSTVAIAARQVVKYMLTDRSTRELMAFTTGEEAVMSIDAG
                     YEESSACSRLDSFFDNENGSLQFVEYNAESPAGIAYQDVLSEVFKSLPVMQEFARQHK
                     LQSLPASNRMLDALLSAFKEWGKSTEPRVAIVDWKGLPTRTEFEMFRKYFKQHGVETI
                     ICAPEDLTYNAKTLYADGQPVNIVYKRVLTSEFLQRFGEEAALEHPLGQAYRDSSIIL
                     VNSFKAKPLHKKMIFGLLTDPVIMDAAKIDRETQLILLKHIPWTRRVQSGMTTYEGQE
                     IDLIKFIKGNQDRLLLKPNDDYGGHGISIGWETDRENWDRAIEKAIETPFVVQERVHI
                     AYEDYPSYIDGKLQIGQRLVDTDPFLFGTEVQGCLTRLSTVTLLNVTAGGGSTTPAFL
                     IS"
     gene            complement(2152..3168)
                     /locus_tag="VFF70_03250"
     CDS             complement(2152..3168)
                     /locus_tag="VFF70_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43743.1"
                     /translation="MPINNNKRIGIMIGREWSWAPAFIEEVAKRGVGVQAEFVKVGGT
                     RMNEANPYAVIVDRISHEIPYYRTYLKNAALQGTIVINNPFWWSADDKFFGASLLDKL
                     GIPHPKTVALPSHSYIDGVVTESLRNLEFPIPWDQHIEYLGGFPVILKPVWGGGFKNV
                     YKVMNLEALFRSYNETGTECMLLQEFIDWDNYARCVCIGQTNIMPIKFDVNRPWPHRY
                     IVDDEFLAPSLRKHVIDYAIQINQALGYDMNTVEFAIKGDVPYAIDFTNPAPDFEVTS
                     LTEHYFPWIVKSMADLCIDLALGKRKQDRSMRWDVLLQAGMKPLESAAPQKPATATRK
                     KKTG"
     gene            complement(3257..3976)
                     /locus_tag="VFF70_03255"
     CDS             complement(3257..3976)
                     /locus_tag="VFF70_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZY43744.1"
                     /translation="MHRSHHQWVSPILNRNMELLVFGHSGAPMIVFPTSQGRFFEYED
                     RGMVGALADPLERGLLQLICVDSVDSESWYCSWAQPSGRIARHIQYDQYILNEVAPFV
                     RQDNRNDFWMTTGCSFGAYHAINFGLRHPDLIRRSIGLSGLYDMRSFMDGYYNQDFYF
                     NNPIDYTANLHDPDLIQRLRQQDIILALGAEDTARWSNDKLSINLWRAGIGNALRIWD
                     GWAHDWPFWDKMIRIYINGHD"
     gene            complement(4148..4687)
                     /locus_tag="VFF70_03260"
     CDS             complement(4148..4687)
                     /locus_tag="VFF70_03260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZY43745.1"
                     /translation="MPAETVDSSEYKYRGHIINLRIDQIHTASGYHGIREIVEHPGAV
                     AIVPIDENEQVILVQQYRHAVGKITIEIPAGTLSPGEDPLVCVRRELKEETGYQAAQI
                     DRIGGVFTAPGISSEFIHLYLATGLTAGESATEEDEAIDLLRVSLSQAIEMIHAGSIN
                     DAKSVSALLLVKEIILPQG"
     gene            complement(4805..>5271)
                     /locus_tag="VFF70_03265"
     CDS             complement(4805..>5271)
                     /locus_tag="VFF70_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY43746.1"
                     /translation="TPLPPQPQATDALGPTALPAQPTNAPAATVAPAQSTPGPSTTST
                     STCGTTYIVQPGDRFFSIGRACNVNPYAIAQANNIGAPYFIYPGQQLIIPGGSPVGAT
                     PVPTSGNPYIVQPGDNLFRIALQFGKTMQAIAAANGLSNINLIFVGQRLTIP"
BASE COUNT         1236 a         1459 c         1326 g         1250 t
ORIGIN      
        1 acgtacgatt gaaactctgc atacgataca aattgtcctg gaaagccggt gcgcggaaaa
       61 ttcgaaaaga taatcgaccg atacgacttg tagccggtgt tgcgtgccat ccagaacggc
      121 gaactcgtcg aaagcatgag caggtgcggc agaaaatatc gagcgacgtt ttgaatttga
      181 atgcccgttt ccaaatccgg catccccacg tgcacatgca tcccgaagat cagcaactgc
      241 cgcgcggcat cttgcatctc attcaacacg ccgtaatagc gttcgtgcgg atacacttct
      301 tgatcggccc aggcggcaaa cggatgtgta cccgccgccg cgatcgtcag tccgttcttc
      361 ttcgctaaac ccgcaattgt tccgcgcaat cgaacgatct ccgcgcgcgc ttcggcgatg
      421 gtgcgacaga catgcgtgcc cgtctcgacc accgactgca tcatctccgg tttcaactgt
      481 tcgcgcaaaa tcaactggcc ttcttccaga atctgcgtca cgtatgatcg cagatcgcgc
      541 gtcaccggat cgatgatttg atattcttct tcgatgccaa ccgtgaaagg aaaatctttg
      601 ctcagaggat catagaaact catgctgcct ccattgtaag gcaaggtcgc ctcgccccta
      661 ccctacgaaa tcaaaaacgc cggcgtggtc gatccgccgc ccgccgtcac attcaacaac
      721 gttacggtcg ataaccgcgt caagcagccc tgcacttcag tgccaaataa aaatggatcg
      781 gtgtcgacta agcgttgtcc gatctgcaac ttgccatcga tgtaagatgg ataatcctcg
      841 taagcgatgt ggactcgttc ctgcacgacg aacggcgttt cgattgcctt ctcgatcgcg
      901 cgatcccagt tttctcgatc ggtttcccag ccgatcgaaa tgccgtgtcc gccgtaatcg
      961 tcgttcggct tcaacaacaa tcgatcttga ttgcctttga taaacttgat cagatcgatc
     1021 tcttggcctt catacgtcgt catgcccgat tgaacgcggc gcgtccaggg aatgtgcttc
     1081 agcaagatca attgtgtctc tcgatcgatc ttcgcggcgt ccatgattac cggatcggtt
     1141 aacaggccga agatcatttt cttgtgcagc ggcttcgcct tgaaactatt cacgaggatg
     1201 atcgagctat cgcggtaagc ctgacccaac ggatgctcta acgcggcctc ttcaccaaag
     1261 cgttgcaaaa attcgctggt caacactctt ttgtaaacga tattgacggg ctgcccatcg
     1321 gcatacaatg tcttagcatt gtacgtcaaa tcttccggcg cgcagatgat cgtttcgacg
     1381 ccgtgctgct taaaatattt ccggaacatc tcaaactcgg tccgcgttgg caaacccttc
     1441 caatcgacaa tggcaacgcg cggctcggtc gatttgcccc attccttgaa tgcgctcaac
     1501 agcgcatcga gcattcgatt cgacgcaggc agtgactgaa gtttgtgttg acgagcaaac
     1561 tcctgcatca ccggcagcga cttaaagacc tccgataaaa catcctgata agcaatccca
     1621 gccggactct ctgcattgta ttcgacgaat tgcagcgagc cattttcatt atcaaagaac
     1681 gaatcgagcc gcgagcacgc actcgattcc tcgtagcccg cgtcgatcga catgaccgcc
     1741 tcttcgcccg ttgtaaacgc catcaactcg cgcgtcgatc gatcggtcag catgtacttc
     1801 accacttgcc gcgccgcgat cgctacggtc gagcagactt cttgaagatg agcgtattga
     1861 tcaactgtga gtaaccgcgg acgcagcaca atgcaaatcg gtcgctcgcc aaagaacaac
     1921 ccttcgcgtt tcgaggccgc caccagcaca tcgttgttcg cccgcgcgct gtcgtcgtct
     1981 aacagactgt gatattcatc gatgatttgt ttaagagtca tagtcccatt gtcgcgtcat
     2041 tgcgaggagc gcaccgcgcg acgaagcaat ctccggatcg actctgagat tgccacgccg
     2101 ccccgaagac cctgcccgaa ctgtgcagag tcggcggctc gcaatgactt gttatcccgt
     2161 tttcttcttg cgtgtcgctg tcgccggctt ctggggtgcg gcagactcga gcggtttcat
     2221 accagcctgc agcagcacgt cccagcgcat cgatcgatcc tgcttgcgct tgccgagtgc
     2281 cagatcgatg cacagatcgg ccatcgactt cacgatccac ggaaagtaat gctcagtcaa
     2341 tgaagtcact tcaaagtccg gcgcggggtt ggtgaaatcg atcgcgtagg gcacatcacc
     2401 tttgatcgca aattcgactg tattcatgtc gtagcccagc gcctgattga tctggatcgc
     2461 gtaatcgatc acgtgtttgc gcaggctcgg cgccaagaac tcatcatcga cgatgtaacg
     2521 atgcggccac ggccgattga cgtcgaactt gatcggcata atattcgtct gaccgataca
     2581 cacacagcgc gcgtaattat cccaatcaat aaattcttgc aacagcatgc actcggtgcc
     2641 ggtctcgttg taggaccgaa acaaggcttc aagattcatg actttgtaaa cattcttgaa
     2701 ccccccgccc cacacgggtt tcaagatcac cgggaagccg cccaagtatt cgatgtgttg
     2761 atcccacgga atgggaaatt ccaaattgcg cagcgattcg gtcaccacgc catcgatata
     2821 tgaatgtgag ggcagcgcca cggtcttcgg atgcggaatg cctaatttat ccaataacga
     2881 tgcgccgaag aatttatcat cagctgacca ccagaatgga ttgttgatca cgatcgtacc
     2941 ttgcagcgcg gcattcttga ggtaagttcg ataatacgga atttcgtgcg aaatccgatc
     3001 gacaatcacc gcgtatggat tcgcttcgtt catgcgcgtc ccgccgacct tgacgaactc
     3061 agcctgcacg cccacgccgc gcttggcgac ttcttcaata aaagccggcg cccatgacca
     3121 ctcgcgtcca atcatgatgc cgatgcgttt gttgttattt attggcatgt tgctcctcca
     3181 tgtgtggatg ttggatgtta gaaattagaa gttggagtga aacctttcca atctccaatt
     3241 tccaacctct gatcaattaa tcatggccgt taatgtaaat ccgaatcatc ttatcccaga
     3301 acggccaatc atgcgcccag ccgtcccaaa tacgcagcgc gttgccgata cccgcgcgcc
     3361 acaaatttat cgagagttta tcattcgacc agcgcgctgt gtcctctgca ccaagtgcca
     3421 gaatgatatc ttgctgccgt agtcgctgga tcagatcagg atcatgcaga ttggccgtgt
     3481 aatcgatcgg attattgaaa tagaaatcct gattgtagta accgtccata aacgatcgca
     3541 tatcgtacag gccgcttagt ccgatcgatc ttcgaatcaa gtccggatgc cgcaaaccaa
     3601 aattgatcgc gtggtacgcg ccgaaggagc agccggtcgt catccagaaa tcgttgcggt
     3661 tatcttgacg cacgaatggg gcgacttcgt tcagaatata ttgatcatat tgaatatggc
     3721 gcgcgatccg gccgctgggc tgtgcccacg agcaatacca actttcacta tcaacactgt
     3781 cgacgcaaat cagttgcagc aagccgcgct ccagtggatc ggctaacgcg ccgaccatac
     3841 cgcgatcttc atactcgaag aagcggcctt gcgatgtcgg aaatacgatc atcggcgcgc
     3901 cgctgtgacc aaagactagc aactccatat tgcgattgag gatggggctg acccattgat
     3961 gatgtgatcg atgcacgtga taatcctttt aatcaacaaa gtgaattggt tggttcaagg
     4021 cggacgaccc tatccgcttc gcaacgaggg caagcagtcc gtcgttcttc aatcgctgga
     4081 ttatcatcca gcgggagcaa aggcgcatat cctaccgcag gtgtctagga cgaaaccaat
     4141 tcttaattta tccctgcggg aggattatct ctttcaccag caaaagtgca ctaacactct
     4201 tcgcgtcatt gatcgatcca gcgtggatca tctcgatcgc ttgcgacaaa gagacacgca
     4261 gcagatcgat cgcttcgtct tcttcggtgg ccgattcgcc cgccgtgagc ccggtcgcta
     4321 aatacaaatg gatgaactcg ctgctaattc cgggcgcggt aaacacgccg ccaatccgat
     4381 cgatctgggc agcctgataa cctgtctctt cttttaattc tcgccgcaca cagaccagcg
     4441 gatcttcgcc cgggctcaac gtaccggctg gaatctcaat ggtgatcttg ccgaccgcgt
     4501 gccggtattg ctgcaccaaa atgacctgct cattttcatc aatcggtaca atcgccaccg
     4561 caccgggatg ctcgacaatc tcgcggatgc cgtgataacc ggaggcagta tgaatctgat
     4621 cgatgcgcag gttgataata tggccgcgat atttatattc ggatgaatcg actgtttcgg
     4681 ctggcatcac tggaagttat tagagaaacc aggtttcggc atgaaacctg gtttctttaa
     4741 gcgaaaaaat tttaagggat aagatcgatc gggtttagct caaccccgat cggttttccg
     4801 atcattatgg aatcgtcagc cgttggccga cgaagatcag attgatattg ctcaagccgt
     4861 tcgccgccgc aatcgcctgc atcgtcttgc cgaattgcaa cgcgatgcgg aacaaattgt
     4921 cgccgggctg cacgatgtag ggattgccgc tcgtcggtac aggtgttgca ccgacaggtg
     4981 agccacccgg aataatcaac tgttgaccgg gataaatgaa gtacggtgcg ccgatgttgt
     5041 tagcttgcgc aatggcgtag ggatttacgt tgcaggcgcg gccaatgctg aagaatcgat
     5101 cgccgggctg gacgatataa gttgtgccgc aagtggatgt ggaagttgtt gacgggccgg
     5161 gtgtcgattg cgcgggggcg acagtcgccg cgggcgcatt ggtcggttgg gccggtaggg
     5221 ccgtcggccc aagcgcgtca gtggcttgag gttgaggtgg caatggtgta c
//
LOCUS       DASRTJ010000154         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_46947,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000154 DASRTJ010000000
VERSION     DASRTJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46947"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFF70_03270"
     CDS             complement(<1..222)
                     /locus_tag="VFF70_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43747.1"
                     /translation="MLKLRVRIHLPIMFLVVIVLLTGLWAALIRVGWQLPNIASAAGY
                     HGALMISGFLGTLVSLERATVLQRKWAYAG"
     gene            complement(393..899)
                     /locus_tag="VFF70_03275"
     CDS             complement(393..899)
                     /locus_tag="VFF70_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZY43748.1"
                     /translation="MKQQTRKMLVIGLSVIAAVILAACGAAATPTAAPTSVPNAGAPS
                     TEEIARPSNPGGPGPALQLTGDAKAGGQIFVDNCQKCHGENGVGGVENPGSDDGTIPE
                     LNPIDDTMVSADLAVFAYNIDLFVEHGSTAPGTDVKEKMTAWGDEKKLTPQQIADVIA
                     YVISLNTK"
     gene            complement(964..2061)
                     /locus_tag="VFF70_03280"
     CDS             complement(964..2061)
                     /locus_tag="VFF70_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZY43749.1"
                     /translation="MRQKENYTRHVALALILTLAVLASFQIYIWGEPGRIASVEALDK
                     EVAVNAGAALFKKNCTLCHGDNGEGASAPALNDKIFLNNTGDDRIFSTVSSGIPNTEM
                     PAWNQSHGGPLTDEDVTQLVAFVRSWEPTAIDRKSLPLVGDIDRGNNIFKNVCGICHG
                     QNGVGTDRAPALNDPAKLSQFDDAWYQNTIEQGRPAKGMPTWGTVLSPQQIADVLAVI
                     DTWRANPITPTPTITATTETTPTVEVARPSNPGGPGSAIGLTGDLKNGSSIFVDNCQK
                     CHGEQGVGNVPNPGSDDGTIPPLNPIDETLIDPDLTVYATNLDLFLEHGSTPAGSNPK
                     EKMPAWGDEQKLTPQQIADVIAYVLSLNPVK"
     gene            complement(2175..2666)
                     /locus_tag="VFF70_03285"
     CDS             complement(2175..2666)
                     /locus_tag="VFF70_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZY43750.1"
                     /translation="MNDTQKKSGVTRRQFLGIAWGASLAGLFGQAGVALLQYLQPRAN
                     ASGFGSRIVAGRPKEFKKNTVSLVQKGHFFISTQDDGGYLAMWQRCTHLGCTVPYRTD
                     EKQFHCPCHGSLFNAQGEVIGGPAPRPLDIFPITLEDGNLVVDTGNPIARDKFDPLQV
                     FHV"
     gene            complement(2895..>3344)
                     /locus_tag="VFF70_03290"
     CDS             complement(2895..>3344)
                     /locus_tag="VFF70_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43751.1"
                     /translation="GILIPSILVAFLIALPYIDQARTGAGVWFSSKRGKRIVGWTALY
                     TAIVMPSYILLDNAFSLREMLRGVVPDWVAQGVLPALIIVVIVALPVLILNRWKPSVR
                     EVMLALFTVMFISAIVLTLSGFLFRGPGFKMYLPWNMPNRYNPFNGL"
BASE COUNT          743 a          980 c          853 g          768 t
ORIGIN      
        1 cccggcataa gcccacttgc gctgcaacac ggtggcacgt tcgaggctga ccagcgtgcc
       61 aagaaagccc gagatcatca gcgcgccgtg ataaccggcc gcggatgcga tgttgggcaa
      121 ttgccagccg acgcggatca acgccgccca taatccggtc agcaggacga tgaccaccaa
      181 gaacataatg ggcagatgaa tacgcacgcg cagttttaac atgaagttat ctcaggttga
      241 tcatagtctc gatcgtcaag aacgtaaatg atttagatca tccaacaaaa aggccgaagg
      301 ttgtggcctt cggcctttca tgtatcgagt aaatgatatc aaagttgggg cagcagttag
      361 actgccgccc cccactctaa cctgtgttga aattacttcg tattcaaact gatgacgtac
      421 gcgatcacgt ctgcaatttg ctgcggggtc aatttctttt catcacccca ggcggtcatt
      481 ttttctttca catccgtacc cggcgcagtt gagccatgct cgacaaagag atcgatgtta
      541 tatgcaaaca ccgcgagatc agcgctgacc atcgtatcat cgatcgggtt cagttccggg
      601 atcgtgccat catccgagcc gggattttcc acgccaccga ctccattctc gccatggcat
      661 ttctggcagt tgtcgacgaa aatttgtccg cccgctttgg catcgccggt cagttgcaaa
      721 gccgggcctg gcccaccggg attcgatggc cgtgcaattt cttcggttga cggagcaccc
      781 gcattcggaa ccgatgtggg tgcagcggtc ggcgtcgccg ccgcaccgca cgccgccaag
      841 atcacggcgg cgatcacact tagtcctatc actaacatct ttcgagtttg ctgtttcatc
      901 acttgcctcc tagttttgat cggaattttt tgaccctaac gatctcttga tcgtgttcac
      961 accttacttc accggattca aactcagcac atacgcaatc acatccgcaa tttgctgcgg
     1021 cgtcaacttc tgttcatcgc cccacgcggg catcttctcc ttcggattcg atcctgccgg
     1081 agttgagcca tgctccaaga acaaatctaa gttagtcgca tacactgtta gatccggatc
     1141 gatcagcgtc tcatcgatcg gattaagcgg cgggatagtg ccatcatccg atccgggatt
     1201 cggcacattg cccacgcctt gctcgccgtg gcacttctgg caattgtcga cgaagatgct
     1261 cgaaccattc ttcagatcgc ccgttaaccc gatcgctgat cccggcccgc cgggattgga
     1321 aggccgcgct acttcgaccg ttggagttgt ttcggttgtt gcggtgatcg taggcgtcgg
     1381 cgtgatcgga ttcgcgcgcc acgtatcaat cacagcgaga acgtccgcga tctgctgcgg
     1441 cgacaagacc gtgccccacg tcggcatccc tttggcggga cgcccctgct cgatcgtgtt
     1501 ctgataccaa gcatcatcaa attgactaag tttcgccgga tcgttgagcg cgggcgcacg
     1561 atcggttccg acaccatttt ggccgtggca gatcccgcaa acattcttga agatgttgtt
     1621 gccccgatcg atatcgccga ccaacggcag cgacttgcga tcaatcgctg ttggctccca
     1681 cgatcgaacg aaggcgacta actgcgtcac atcttcatcg gtcaaaggcc cgccgtgcga
     1741 ctggttccac gccggcattt cagtattcgg aatgccggaa ctaaccgtgc tgaagattcg
     1801 atcgtcgccg gtgttgttca aaaatatttt gtcattcaag gccggggcac tggccccttc
     1861 accgttatcg ccatgacaca acgtgcagtt tttcttgaac aacgcagcgc ccgcattcac
     1921 cgccacctct ttgtcaagag cttcgacgct ggcaattcgc cccggctcgc cccaaatata
     1981 tatctggaat gaggccagga cagccagcgt gagaatgagt gcgagggcga cgtgccgggt
     2041 atagttttct ttttggcgca taagttgcct cgaatgattc ggttagcgct ttaggttatc
     2101 accctgagcg cagcgaaggg tctcgcacaa ttatccgaga tgcttcgcta cgctcagcat
     2161 ggcaggcgta aaacctaaac atgaaaaacc tgcaatggat cgaatttgtc gcgcgcgatg
     2221 ggattgccgg tatcgacgac gagattgccg tcctccagcg tgatcggaaa aatatccagc
     2281 gggcgcggcg ccggtccgcc gatcacttca ccttgcgcgt tgaacaacga gccgtgacac
     2341 ggacaatgaa actgtttctc gtcggtgcga tacggcacag tgcagcccaa gtgcgtgcag
     2401 cgctgccaca tcgccagata accgccatcg tcttgcgtcg aaataaagaa gtggcctttt
     2461 tgcaccaggc tcaccgtatt cttcttgaac tctttcggac ggccggcgac gatcctgctc
     2521 ccgaagccgc tagcattggc gcgcggctga agatattgca gcagtgcgac tccagcttgt
     2581 ccgaacagac ccgccagcga agcaccccac gcgatgccta aaaattgtcg gcgcgtcacg
     2641 ccggatttct tttgagtatc gttcattcat tccctccgac ttgcgtcaga tgtttttcat
     2701 ttaccttcac tacgcagccg tcattgcgag gagcgcagtt tgcgacgaag caatctctgt
     2761 cactgatagg gacactcgcc gatagtcgct gagattgctt cgcaaaaagc gctcgcaatg
     2821 acgctacctg agcagttacc atttgtcatt gttcattcgt cattgttcat tgttacttgt
     2881 tcattgctaa ttgtttacag tccgttgaac gggttatacc tattcggcat gttccatggc
     2941 agatacatct tgaagcccgg cccgcggaat aagaagccgc tcaacgtcag cacgatcgcc
     3001 gagatgaaca tcacggtgaa caacgccagc atcacttcgc gcacgctcgg cttccagcga
     3061 ttcaaaatga gaacgggtaa tgcaacaatc accacaatga tcaacgcggg cagcacaccc
     3121 tgtgcgaccc aatcgggcac gacgccgcgc agcatttcac gcagactgaa cgcgttgtcg
     3181 agcaggatgt agctcggcat gacgatcgcg gtatacaacg ccgtccaacc gacgatccgc
     3241 ttgccgcgct tcgatgagaa ccacacgccc gcgcccgttc gcgcctgatc gatgtacggc
     3301 agcgcgatca agaacgcgac gaggatcgac gggatcaaga tacc
//
LOCUS       DASRTJ010000155         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_268898,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000155 DASRTJ010000000
VERSION     DASRTJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268898"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..826)
                     /locus_tag="VFF70_03295"
     CDS             complement(146..826)
                     /locus_tag="VFF70_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZY43752.1"
                     /translation="MYGSGVIKGMFVTLKHFFVSYIEDLKYLFRGGRYTDAAIPARQG
                     PKTRGIFTVQYPEERNPVPENFRYIPFLIYDTQKNDDKCTSCGICAKVCPPQCIWIVR
                     TNDPKTNRPVPQPAEFYIDASICMSCGLCAEFCPFDAIKMDHDYELSSFERYDTMVYD
                     KARLTKPDTYHAAIHPTDWKREQQEVIEKAAKDAEKAAAAAKAAAAKAAAAAAAPKTD
                     APPAVTPA"
     gene            complement(839..1984)
                     /locus_tag="VFF70_03300"
     CDS             complement(839..1984)
                     /locus_tag="VFF70_03300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZY43753.1"
                     /translation="MSRNGNGQHAGEDFATDQLVVNLGPQHPSTHGVFRMVVTLEGES
                     IVRLEPVMGYLHRNHEKIGERNTYIQNMPYTDRLDYISSMSNNLGYALAVEKLMKIAP
                     PERAEYIRILMVELTRICNHMWAIGFLLNDLGAFFTPALYAITERELILDLFESASGS
                     RMMCNYMRFGGVTRDLPEGWIDTCRELVMNRLPKAIDDLDHFLSDNEIIRARCEGVGV
                     LTAEDAIAYSMAGPVLRASGVPYDVRRAEPYSIYDRFDFDVAVRYHGDVYDRYYIRID
                     EMRESVRILQQALQQMPTGGEIQTGKKQYQTRVPAGHAYGRVENPKGELGFYVVADGN
                     PNPYRYHIRAPSFINLTALGKMCVGQKIADVVIVLGSIDIVLGETDR"
     gene            complement(2195..2494)
                     /locus_tag="VFF70_03305"
                     /pseudo
     CDS             complement(2195..2494)
                     /locus_tag="VFF70_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
BASE COUNT          556 a          712 c          657 g          572 t
ORIGIN      
        1 atcatgacag atgcaactgc cagaatctcc gtccgtcata aatcaggcgc gaatgcgtcg
       61 cccctacaaa ttgattttaa caaaaccgcc ctcgcccgga acgatccagc gagagcggtc
      121 tgtggttatc cgtaaagcta aaaccttaag caggtgttac cgctggcgga gcatcggtct
      181 tgggcgcagc tgcggccgcg gccgcttttg cagccgcggc tttggcagcg gctgcagctt
      241 tctccgcatc cttcgccgct ttctcaataa cttcttgctg ctcgcgtttc caatcggtgg
      301 gatggatcgc cgcgtgatag gtgtcgggtt tggtcaagcg ggctttgtca tacaccatcg
      361 tatcgtagcg ttcaaaactc gacagttcgt aatcgtgatc cattttgatc gcatcgaacg
      421 ggcagaactc ggcgcacagg ccgcacgaca tacagatcga cgcatcgatg tagaactcgg
      481 caggctgcgg aaccggccga ttggttttgg gatcattcgt ccgcacgatc cagatgcact
      541 gcggcggaca aactttggca cagataccgc acgaggtgca tttgtcgtca ttcttctgag
      601 tgtcgtatat caagaatgga atgtagcgaa aattttccgg aaccggattg cgctcttcgg
      661 gatattgcac ggtgaaaatg ccgcgcgtct tcgggccctg ccgcgccgga atcgccgcgt
      721 cggtatagcg accgccccgg aaaagatatt tcaagtcctc aatatacgac acgaagaaat
      781 gcttcaaggt gacgaacatg cctttgatga caccagaacc gtacatgctt gctccttctt
      841 accgatcggt ttcgccgagc acgatatcga tgctgcccaa cacgatcacc acatccgcga
      901 tcttttggcc gacgcacatc ttgcctaacg ccgtcaaatt gataaacgat ggggcgcgga
      961 tgtgataccg atacggattc ggattcccat cggccacgac atagaagccc aactcgcctt
     1021 tcggattttc gacgcggcca tacgcatggc cggccggcac gcgcgtttga tattgtttct
     1081 tgccggtttg aatctcgccg ccggtcggca tctgctgcaa ggcttgctgc aaaatgcgca
     1141 ccgattcgcg catctcatcg atgcgaatgt aatagcgatc gtatacatca ccgtgataac
     1201 gcaccgccac atcgaagtcg aatcgatcgt agatcgaata cggctcggcg cggcgtacat
     1261 catacggcac acccgaggcg cgcaggacag gcccggccat gctatatgca atcgcatctt
     1321 ccgccgtcag gacgcccacg ccctcacaac gcgcgcggat gatttcgttg tcgcttagga
     1381 aatgatcgag atcgtcgatc gctttcggca atcgattcat caccaactcg cggcaggtgt
     1441 cgatccagcc ttccggcaaa tcgcgcgtca cgccgccaaa gcgcatgtaa ttgcacatca
     1501 tgcgcgagcc tgaagccgat tcaaacagat cgaggatcag ttcacgttca gtaatcgcat
     1561 acaacgcggg cgtgaagaaa gcgccgaggt cgttcagcaa gaacccgatc gcccacatgt
     1621 gattgcagat tctcgtgagc tcaaccatga ggatgcggat atattcggcg cgctcaggcg
     1681 gtgcgatctt catcaatttc tcaaccgcca gtgcgtagcc caggttgttg ctcatcgacg
     1741 agatgtaatc caaccgatcg gtgtacggca tgttctgaat ataggtattg cgttcgccga
     1801 tcttctcgtg attgcgatgc agataaccca tcaccggctc aagccgaacg atcgattcgc
     1861 cttcgagcgt aacgaccatg cggaacacgc cgtgcgtcga ggggtgctgc ggtccgagat
     1921 tgacgaccaa ctgatcggtc gcgaaatctt cgccggcatg ttggccgttg ccgttgcgtg
     1981 agatcgtgcg accttgatcg tcgacatcga cattcttgaa gtttgccatg accgaggttt
     2041 cggcgatttc attgaacttc accggatcaa aatccgccgg atacttgata ttatccgcaa
     2101 acggcactcg atcttcaatg cgtgtgtgat gcccgcccgg ccagcggttg tcaaagggtt
     2161 tggtgtcctg ctcgtagtag gcttcgtgcc aatccttgcg cagaggatgc ccttcaaaac
     2221 cttcccacat caaaatgcgc ttgagattgg gatggccttc atattgaatg cccatcaagt
     2281 cccaagcttc gcgctcttga aaatcggcgc cgggccagat cgatgtcacc gacgggatca
     2341 cactgttgtc gcgcggcgcg cgcgctttaa tgaccaatgg gccgccgcct ttgtgcaggc
     2401 cgtgcttggt cgaatacaaa tgatagaccg attcgacttc atttgtggac ggataatcta
     2461 cgcccgtcac actcgataaa taatcgtagc ccaattc
//
LOCUS       DASRTJ010000156         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_84429,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000156 DASRTJ010000000
VERSION     DASRTJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84429"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1432
                     /locus_tag="VFF70_03310"
     CDS             170..1432
                     /locus_tag="VFF70_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HZY43754.1"
                     /translation="MTLRHRIRPFRARLLLLLAVIGPGIITANVDNDASGITTYSVAG
                     AHYGYSLLWLMPLVALALIVVQEMSARLGIVTGKGLADLIRENIGFRWTTIIIGLMLI
                     ANLANTVSEFAGVAASMEIFGISKFVSVPVVALGVWLLIVKGSYKHVERIFLIASALY
                     LTYIASGIIAHPPWDQVARAAITPTFHFDVGYVTIFVTIIGTTIAPWMQFYQQASIVD
                     KGLKATELSYERIDVILGSVFAVVVAAFITIACAATLFANGIQIATAEDAALALRPLA
                     GVNASTLFAFGLFNASVFSAAILPLSTAYVVCEAFGWEMGVSRSFKEAPIFFSIYTAL
                     IALGAGIILLPVRSLIDTMLSSQTLNGVLLPVILIVMLRLINNKRLMGRYVNGRLFNL
                     LAWGTVVILIGLTLILIVTSWFPQVLSR"
     gene            complement(1508..1891)
                     /locus_tag="VFF70_03315"
     CDS             complement(1508..1891)
                     /locus_tag="VFF70_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZY43755.1"
                     /translation="MGKKLYVGNLPYSITEDEVRAMFAEVGTVTSVTLITDRATGQPK
                     GFGFVEMETEQAAQEAITKLNGREMQQRTLNVSEARPMAERSGGGGRGGYSGGGGSSR
                     GGGGSGGRGGSRGGGGGDRDRGGRY"
     gene            complement(1919..2128)
                     /locus_tag="VFF70_03320"
     CDS             complement(1919..2128)
                     /locus_tag="VFF70_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZY43756.1"
                     /translation="MSERIQGTVKWFNATKGYGFIAREGGEDVFVHHSAIQAEGYRSL
                     NEGQRVEFTLERGPKGLQASNVVPL"
     gene            2610..>2831
                     /locus_tag="VFF70_03325"
     CDS             2610..>2831
                     /locus_tag="VFF70_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43757.1"
                     /translation="MNLDFLNYLPIIIIVVVVVILAIVFLPMLRGSMRSSKLMQTGVS
                     APAQILKVWQTGMTINDQPQIGFLLQVQPA"
BASE COUNT          582 a          808 c          746 g          695 t
ORIGIN      
        1 agcatggaag aagcggctgc ccagactata tcgctaaacg actacgaatg acgcaccgca
       61 ctccaccagc gctatgtggg ctatgcgacc gaccctgatc gtttagtaaa acaagcattc
      121 aatttcgatc actcactctt cactcttcac tcgttactca ttactttcca tgactctgcg
      181 ccatcgtatc agacctttcc gcgcccgatt gttattgctg ctggccgtca tcgggccggg
      241 cattattacc gccaatgtcg ataacgacgc gagcggcatc accacgtact ccgtcgcggg
      301 agcgcactac ggctatagtt tgctgtggtt aatgccactg gtggcattag ctttgattgt
      361 ggtgcaagag atgagtgcgc gcctggggat cgtcacgggc aaagggctgg ccgatctgat
      421 ccgcgagaat atcggcttcc gctggacgac gatcatcatc ggcttgatgt tgatcgcgaa
      481 cctggcgaat accgtcagcg aatttgccgg cgtcgccgcc agcatggaaa ttttcggtat
      541 cagcaaattc gtctcggtgc cggtcgtggc gctcggcgtg tggctgttga ttgtcaaagg
      601 cagctacaag catgtggagc gcatcttctt gatcgccagt gcgttgtatc tgacctatat
      661 cgcttcgggc attatcgcgc atccgccctg ggaccaggtg gcgcgagcgg ccatcacccc
      721 cactttccat ttcgatgtgg gttacgtaac gatctttgtg acgatcatcg gcacgacgat
      781 tgcgccgtgg atgcaatttt atcaacaggc gtcgatcgtg gataaaggac tgaaggcgac
      841 cgaactatct tacgagcgga tcgatgtcat cctcggctcg gtctttgccg tggtggtggc
      901 agcgttcatc accatcgctt gtgcggccac cttatttgcc aatggaattc agatcgccac
      961 cgccgaggac gcggcgttgg cactgagacc cttggccggc gtgaatgctt ccacactctt
     1021 tgcgtttggt ttgtttaatg cctcagtgtt ctcggcggcg atcttgccgc tgtccacagc
     1081 ctatgtggtg tgtgaagcgt tcggttggga gatgggggtc agtcggagtt tcaaagaagc
     1141 gccgatcttt ttcagcatct acacggcctt gatcgcattg ggcgcgggca tcattctgct
     1201 gccggtgaga tcgctaatcg atacgatgct gtccagccaa acgctgaatg gcgtactctt
     1261 gccggttatc ctgatcgtga tgctgcgcct gatcaacaac aaacgcttga tgggccgcta
     1321 tgtcaacggc cgcctcttta atctgctggc gtggggcacg gtggtcatct taatcggcct
     1381 gacgttgatc ttgattgtca ccagctggtt cccgcaagtg ctcagccggt gaacattcaa
     1441 cgtgcctaaa tcgaaaaagc gcagagacac tgtctctgcg cttttattgt agtgatgagt
     1501 tgaattttta gtagcgaccg cctcgatcgc gatcaccacc accgccgccg cgcgaaccac
     1561 cgcgaccgcc gctgccgcca ccaccgcgac tgctaccacc gccgccgctg tagccgccac
     1621 gaccgccgcc gcccgatcgc tcggccatgg ggcgcgcttc gctcacattg agcgtgcgct
     1681 gctgcatttc gcggccattc aatttagtaa tggcctcctg cgctgcctgt tcggtttcca
     1741 tttcgacgaa cccgaaacct ttcggctggc ccgtcgcccg atcggtgatc agtgtgacgc
     1801 tggtgaccgt gccgacttcg gcaaacatgg cgcgcacttc atcttcggtg atgctgtacg
     1861 gcaaatttcc gacatacaat ttctttccca tggaaagttc ctttcgctcc gctgtaaatt
     1921 acaacggcac gacgttgctg gcttgcagcc ccttggggcc gcgctccagc gtgaactcaa
     1981 cgcgttggcc ttcgttgagg gatctatagc cttctgcttg gatggcactg tggtggacga
     2041 agacatcttc gccgccttcg cgcgcaataa agccgtagcc cttggtggca ttgaaccact
     2101 taaccgttcc ctgaatccgt tcgctcatac tgctccttaa agaaagtgat ttgcgttagg
     2161 tctaaagagg ttcgccgccg gaaaagggaa atgcccacta agatgaaaga cctagcgggc
     2221 attcgtactt cgagccgaac aggaactttc ctcatcctac gccacgcact ttaccacaga
     2281 gccaagcccg agtcaagcgg caattgacga ctttggaatc ttctgaccgc atcttcaccc
     2341 ggctgaaacg caagtctctc cagtcccggc ctggactatc aggctgaaaa actcacgaat
     2401 cgattggaaa taaagtcatg tgatacaagc aaagctatgc tagagtcagg tcgatcgatg
     2461 gtctgaccgt acagctggca gcgagaagaa ttatgctatt gacttttgtt gggatttcca
     2521 tgaaaatata tccacgcccg attgattgat ccggccgatc gggctggcgt gaacgatcga
     2581 ggccaaaaac ttttccacag gagactatca tgaatcttga cttcctcaac tatctaccga
     2641 ttatcatcat tgtcgtcgtc gtcgtgatcc tcgccatcgt cttcctgcct atgctgcgcg
     2701 gctcgatgcg atcgtccaag ttgatgcaaa cgggtgtttc agccccggct caaatcctca
     2761 aagtctggca gaccggcatg actatcaacg atcagccgca gatcggtttc ttgctgcaag
     2821 tgcagccggc g
//
LOCUS       DASRTJ010000157         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_236145,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000157 DASRTJ010000000
VERSION     DASRTJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236145"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..437)
                     /locus_tag="VFF70_03330"
     CDS             complement(42..437)
                     /locus_tag="VFF70_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43758.1"
                     /translation="MSISKYKKIFAALAVIALIGLAVGARSNRLASAQSGVGGINPPF
                     TFHETVAPNSVELGFPPIGGTVIFTETFGPSFAPTTTLVTSTAMWHAIINPDVASDYY
                     WGRVSAGAFNNTAWNAASSITSTALTRPQ"
     gene            complement(473..1204)
                     /locus_tag="VFF70_03335"
     CDS             complement(473..1204)
                     /locus_tag="VFF70_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZY43759.1"
                     /translation="MRLIRNDKFIKQRAGLGRYVSFGGMGILIVGLLISFSSPELFYI
                     SFLCLLVGFLCSQIGIYLTNRYARLDRPDEILAKALKGFDDRYALYEYTSPAGSVFLT
                     PNACIVFTVKSQSGSIEFRDGKWRHQTGGLRRIFGWMTQEGIGNPIRDAQAEAGALQR
                     FLSKKLPDVEVQIQPMIVFGSPKAEVNAADSPIPAVHAKKLKEWLRSHAKSGSLDADT
                     HDRLALLFEIPEATHKPADSSETEE"
     gene            complement(1173..2486)
                     /gene="fabF"
                     /locus_tag="VFF70_03340"
     CDS             complement(1173..2486)
                     /gene="fabF"
                     /locus_tag="VFF70_03340"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZY43760.1"
                     /translation="MELRPDAQRVVITGLGAISPLGHTVAEYWDGLIHGRSGIGKITQ
                     FDASEFPTQIAGEVKDFDVSKWMNIKEARRIGRASQLAFATAHEALTDAGMGLPFPEE
                     LQEDAGVLIGTAMGNFDKADEEMQIFRKKGWRFASPFGITAAMPNMPSHHVSMLAQAK
                     GPISTVVSACATGTQAIGEAAEHIRRGAAKIMISGGVEGMIRPYGIGGFNVMRALSTR
                     NDDPEGASRPFDKTRDGFILSEGCGLLVLESLEHARSRGAKIYAEVLGYSSSSDAYHV
                     AAPDPTAAGAVRAMKWALRDARVEPHEVSYINAHGTSTPVNDAVETLAIKKLFGEQAY
                     NIPISSSKSMIGHCMGAAGALEAIACTQIIQEQMIHATINYVTPDPDCDLDYVPNTPR
                     KSNIKVTLSNSFGLGGNNACLVLGAYSQNGTGHSSHATHPQRQIH"
     gene            complement(2489..>3388)
                     /gene="plsX"
                     /locus_tag="VFF70_03345"
     CDS             complement(2489..>3388)
                     /gene="plsX"
                     /locus_tag="VFF70_03345"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZY43761.1"
                     /translation="LPISIVQASEVVEMHEHTLAVKEKKDNSLSVGARLLKEGKADAM
                     MSAGNSGAVMAAALFGLGRIRGIDRPALCSKFPAGDTLTVLIDLGANTDPKPINLLQN
                     ALMGSIYAEHVLGMKNPRVGLVSIGEEADKGSMLVRDAYKLFKESSLNFVGNIEGKDI
                     PRSAADVIVTDGFTGNVITKLTEGIASFMIRFVKRELTGGLLNKIAFVLMIPGLILML
                     PGIILLSPSLRRLRKKVDYREIGAAPLLGVDGIVMIAHGRSDAQAIFGAIRSTQQAVD
                     AKIIEAIKAGLADSKMAASRIES"
BASE COUNT          755 a          933 c          903 g          797 t
ORIGIN      
        1 atgtggcacg cgatcatcaa tcccgatgta gcgtcggatt attattgggg tcgcgtgagc
       61 gcggtgctgg taatagagct ggctgcattc caagcggtgt tgttgaatgc accagcgctc
      121 acgcgacccc aataataatc cgacgctaca tcgggattga tgatcgcgtg ccacattgca
      181 gtcgatgtga ccagcgtagt cgttggtgcg aaagacggtc cgaaagtttc agtgaagatc
      241 actgtgccgc cgatgggtgg aaagccgagc tctaccgaat ttggggccac tgtttcgtga
      301 aacgtaaacg gcggattaat tccgccaacc ccagattgag ctgaggccaa tcgattggat
      361 ctggccccga cggctaagcc gattagagca atgacagcca gcgccgcaaa tattttcttg
      421 tattttgata tagacatttg atgaccacct tttgttaatg gagggttgcc gattattctt
      481 cagtctctga cgagtcagcc ggtttatgag ttgcttctgg aatttcaaac agcaaggcta
      541 atcgatcgtg cgtgtcagcg tccaggctgc ctgacttggc gtgactgcgc agccactctt
      601 tgagtttctt cgcgtgcacc gccgggatag gcgaatcggc cgcattgact tctgccttcg
      661 ggctgccgaa tacaatcatc ggctgaatct gaacttcaac gtccggtagt tttttggaga
      721 ggaaacgctg caaggcgccg gcctcggctt gagcatcgcg tatcggattg ccgatgcctt
      781 cttgtgtcat ccaaccgaat attcgccgca ggccgccggt ttgatgccgc cacttgccgt
      841 cgcgaaattc gatcgagcct gattgcgatt tcactgtaaa gacaatgcag gcattaggtg
      901 tcaagaagac gctgcccgcc ggtgaagtat attcatacaa tgcgtagcga tcgtcgaagc
      961 ccttgagcgc ctttgctaga atttcgtcag gccgatcgag gcgcgcgtat cgattggtga
     1021 gataaatgcc gatttgtgag cacaagaagc cgacgagcaa acacagaaat gaaatgtaaa
     1081 acaattccgg gctgctgaat gaaatgagca agccgacgat caagatgccc atgccgccga
     1141 aactgacata tcgaccgagg ccggcgcgct gcttaatgaa tttgtcgttg cggatgagtc
     1201 gcatgagatg agtgtcctgt tccgttttga gaatacgcgc ctaagaccag gcaagcattg
     1261 ttgccaccca aaccaaagga attggataat gtgactttga tgttggactt gcgcggtgta
     1321 ttgggcacgt aatcgaggtc gcagtcgggg tcgggtgtga cgtagttgat cgtcgcatgg
     1381 atcatttgct cttgaatgat ctgcgtgcag gcgatggcct ccagcgcgcc ggccgcgccc
     1441 atgcagtgcc cgatcatcga cttggaggaa ctgatcggaa tgttgtaggc ttgctcgcca
     1501 aacagttttt tgatcgctaa agtttccacc gcatcattga ccggtgtcga ggtgccgtgc
     1561 gcgttaatgt aactgacttc atgcggctcg acacgcgcat cacgcaatgc ccacttcatc
     1621 gcgcggactg ccccagccgc tgtgggatcc ggtgcggcaa cgtgataagc atcagaggat
     1681 gaagagtagc ccagcacttc ggcgtaaatt ttcgccccgc gcgatcgggc gtgctccaaa
     1741 ctttcgagca cgagtaaccc gcagccttca ctcaaaataa atccgtcgcg agttttatcg
     1801 aatgggcggc tggcaccttc cggatcgtca ttgcgagtcg agagcgcacg cattacattg
     1861 aagccgccaa tgccatacgg gcggatcatg ccttcaacac cgccgctgat catgatcttc
     1921 gctgcaccgc gtcgaatgtg ctcagccgct tctccgatcg cttgagtgcc cgtcgcgcat
     1981 gcgctgacca cggtgctgat cggccccttg gcctgcgcaa gcatactcac atgatgtgac
     2041 ggcatgttgg gcatggcggc tgtaatgccg aaagggctgg caaaccgcca gcctttcttc
     2101 cgaaagattt gcatttcttc atcggcttta tcgaagttac ccattgccgt gccgatcaag
     2161 acgccggcat cttcttgaag ttcttcggga aaaggcagac ccatgccggc atcggttaag
     2221 gcctcatgcg cggtagcgaa cgcaagttgc gaagcgcggc cgatacgccg tgcttcttta
     2281 atgttcatcc atttgctgac atcgaagtct ttgacttcac ccgcgatctg cgtgggaaat
     2341 tcactcgcgt cgaactgtgt gatcttgccg atgccggatc ggccgtggat caatccgtcc
     2401 caatattctg ccacggtgtg acccagcggc gagatcgcgc ccaaaccggt aatcacgacc
     2461 cgctgagcat ccggtcgaag ttccatttct aagattctat ccttgaagca gccattttgg
     2521 aatcagctaa accagcctta atcgcctcaa taattttggc atcaaccgcc tgctgggtcg
     2581 atcggatggc accgaagatc gcttgtgcat ccgatcggcc gtgcgcgatc atgacgatgc
     2641 catccacacc cagtagcggc gccgcgccga tttcacggta atcgactttc ttgcgcaacc
     2701 gtcgcagcga gggactaagc aaaataattc ccggcagcat caagatcaaa ccgggaatca
     2761 tcaacacgaa cgcgatctta ttcaacagcc cgccggttaa ttcgcgcttg acgaagcgaa
     2821 tcataaaaga ggcaatgcct tcagtgagtt tggtgattac gttgccggta aagccgtcgg
     2881 tgacgatcac atccgccgcg ctgcgcggaa tatctttgcc ttcgatgttg ccgacaaagt
     2941 tcaatgaact ctctttgaaa agtttatacg catcgcgcac caacatcgag cctttatcgg
     3001 cttcttcgcc gatggagacc aacccaaccc gcggattctt catccctaaa acatgctcgg
     3061 cgtaaatgct acccatcagc gcgttttgca gcaagttgat tggcttggga tccgtattcg
     3121 cgccgagatc gatgagcacc gtcaaggtgt cgcccgccgg aaacttagag cacaacgccg
     3181 gtcgatcgat gccgcgaatg cggcctaatc caaacaatgc cgccgccatc actgcgcccg
     3241 aatttcccgc cgacatcatc gcatcggctt tgccctcttt caataagcgc gcgcccacgc
     3301 tcaacgaatt gtctttcttt tctttcactg ccagcgtgtg ctcgtgcatc tccacgactt
     3361 cactggcttg gacgatcgag atcgggag
//
LOCUS       DASRTJ010000158         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_236173,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000158 DASRTJ010000000
VERSION     DASRTJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236173"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFF70_03350"
     CDS             <1..187
                     /locus_tag="VFF70_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43762.1"
                     /translation="KSTASSNPLQEFASLTQSIGNLGAVLSDGADASVLQESLQTILK
                     RVKHVAEAAGVTLTSNE"
     gene            277..915
                     /locus_tag="VFF70_03355"
     CDS             277..915
                     /locus_tag="VFF70_03355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZY43763.1"
                     /translation="MTKHFIAIAGNIGVGKSSLVGLLADRLHWTPFYEAVDENPYLSD
                     FYRDMRSWSFHSQIFFLSKRLRHHRALLDHPGSVIQDRSIYEDAEIFAKNLYRQGNIS
                     ERDYATYRELYEVLTLYLPPPDLMVYLRASPDTLKKRITLRGRDYERDIAADYLLRLN
                     DLYEEWINAFTLCPILTVPADKFDYVHQDGHLDIVTSTILKRLEGQEVVRFD"
     gene            959..2125
                     /locus_tag="VFF70_03360"
     CDS             959..2125
                     /locus_tag="VFF70_03360"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZY43764.1"
                     /translation="MSFLTHLECSECHRTYDPNVLQTICPEDKSPLLVRYDLDRAKHE
                     LDRDQFGSPRPNSLWRWFELLPIDDPADRVDLGEGGTPQLHINRIGSQLGFKRIWIKD
                     EGQNPTGSFKARGMAVAVTRAKILDVKEFVVPTAGNAGGAASAYVARAGLKIHVYMPK
                     DAPRANIDECRAAGADVQLIEGLINAAGRIAAEDALRNGWFDLATLKEPYRIEGKKTM
                     GYEIAQDFGWKLPDVIIYPAGGGTGLIGIWKAIDELQQLGWIDSKRPRMVAVQAIGCQ
                     PIVKAFAENKEASELYPNAQTIAAGLRVPKPLGDRLILRALRESAGTAVAVSDQDIID
                     MTQRLVRTEGLSMAYEGAATIVAAQQLLDRGWIKSDEQVLCLNTGAGWKNQAVG"
     gene            2389..>2990
                     /locus_tag="VFF70_03365"
     CDS             2389..>2990
                     /locus_tag="VFF70_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY43765.1"
                     /translation="MTSSPQPPAQRSDSSPEPIPSHGLTATNKNLADRFKQVRVPKTF
                     RALQYRNYQLYFGGQLISVAGTWMQSIAQAWLVYQLSHSELMLGVVGFAAAIPALIIT
                     PIGGVIVDRVPKRALLVITQSCAMLLAFILALLSFLNVVQVWHIVALACGLGIVNAFD
                     GPGRQAFVVEMVGRDDLPNATAVNSMMFNAARVIGPAIGG"
BASE COUNT          677 a          818 c          794 g          701 t
ORIGIN      
        1 taaatcaacc gcgtcttcaa atccactgca agaattcgcc tcgcttactc aatcgatcgg
       61 gaacctcggc gcggtcttgt ccgacggcgc cgatgcgagt gtgctacaag agtcgctgca
      121 aacaatcttg aaacgtgtga agcatgtggc cgaggcggcg ggagtcacac tcacgagcaa
      181 tgagtaacaa gtcatgctac attactcgtt actcatcact ttccatcaaa cttaaatatt
      241 cctttaacga tacagcttac tcagtaaggg tgttccatga ctaaacattt catcgccatc
      301 gcgggtaaca tcggcgtcgg caagtcatcg ctggtcggtt tgttagccga tcgattacac
      361 tggacgccgt tctacgaagc cgtcgacgag aatccgtatt tgtccgattt ctatcgagat
      421 atgcggtcgt ggagttttca ttcgcaaatc tttttcttat cgaagcgctt gcgtcatcac
      481 cgcgccttgc ttgatcatcc cggctcggtc atacaagatc gcagcatcta cgaagacgct
      541 gaaatctttg ccaagaattt gtatcggcag ggcaatatca gcgagcgcga ctacgcgact
      601 tatcgcgaac tctatgaggt cctgacgctg tacttaccgc cacccgatct catggtctat
      661 ttgcgcgcca gccccgatac attgaagaaa cggatcacac tgcgcggccg cgattatgag
      721 cgcgacatcg cagccgatta tctactgcgc ctcaacgatc tgtacgaaga atggatcaac
      781 gccttcacgc tctgcccaat cttaaccgtg cccgccgata aattcgatta cgtgcatcaa
      841 gacggccacc tcgatattgt gacttcgact attttgaaac gattggaagg ccaagaagtt
      901 gtgaggtttg attaatcgta gggcgagcgg ccgctcgccc tactcgcgaa ggtcaagcat
      961 gtcctttctt actcacctcg aatgttccga atgccaccgc acttacgatc cgaatgtctt
     1021 gcagacaatc tgccccgaag ataaatcgcc gctcttagtt cgctacgatc tcgatcgggc
     1081 caagcacgaa ctcgatcgcg atcagtttgg atcgccgcga ccgaatagct tgtggcgctg
     1141 gttcgaacta ctcccgatcg atgatccagc cgatcgagtt gatctgggcg aaggcggcac
     1201 gccgcagctg catatcaatc gcatcggatc acaactcggc ttcaagcgga tctggatcaa
     1261 agacgaaggg caaaatccaa ccggatcatt caaagcgcgc ggcatggctg tggctgtgac
     1321 ccgcgcgaaa atattggacg tgaaagaatt tgtcgtgccg acggcgggca acgcgggcgg
     1381 cgcggcctcg gcctacgtgg cacgtgctgg cttgaagatt cacgtctaca tgccgaaaga
     1441 tgcaccgcgc gcgaacatcg atgagtgccg cgcggctggc gcagatgtgc agctgatcga
     1501 agggctgatc aacgctgcgg gtcgtattgc tgccgaagac gcgctacgaa atggctggtt
     1561 tgatttggct acactcaaag aaccgtatcg cattgaaggc aagaaaacga tgggctatga
     1621 aatcgcacag gatttcggct ggaagctgcc ggatgtgatc atttatccag ccggcggtgg
     1681 cacgggcttg atcggcatct ggaaagcgat tgatgagctg cagcaactgg gctggatcga
     1741 ttcgaagcgt ccgcgcatgg tcgcggttca agcgatcggc tgccagccga tcgtcaaagc
     1801 cttcgctgag aataaagagg caagtgagtt gtatccgaat gcccagacga tcgccgccgg
     1861 gttgcgtgtg cccaagccgc taggcgatcg cttgatcctt cgtgcgctgc gtgagagcgc
     1921 tggcacagcc gtggctgtat ctgatcaaga tattatagac atgactcaac ggcttgttcg
     1981 gactgaaggc ctgtcgatgg cttacgaagg tgcagccacc atcgttgctg cacagcaatt
     2041 actcgatcga ggctggatca aatcggacga gcaggtgttg tgcttaaaca cgggtgcagg
     2101 ctggaaaaat caggcagtgg gttagtgact gctggtccgc atttaacgtt taagccacag
     2161 ttgaactgcg gcggcagcag tgcaactact gccgcaactg cataatgtga atattctaca
     2221 ctacacttct ctcatttttg atcgtttagc ctattgcatt cagcgcgagt tggatgtaaa
     2281 cttagtgcag gtcaggggtt ttgtgcggca tacgcgctat acttatagca tatatgccgc
     2341 acctgttttg ccatcaactt caaccgcctc caattggatc aggatacttt gacttcgtca
     2401 ccccaaccac ccgctcagcg atcagatagc agccccgaac ccattccatc tcacggacta
     2461 acggccacta acaaaaatct ggctgatcgc ttcaaacaag tccgcgttcc aaaaactttc
     2521 agagccttgc agtatcgtaa ctaccaattg tatttcggcg gacaactcat ctccgtcgcc
     2581 ggcacgtgga tgcaatcgat cgcccaggcg tggctggttt accagctcag ccactccgaa
     2641 ctcatgctgg gcgtggtcgg ttttgccgcg gcgattccag cgctcatcat tacaccgatt
     2701 ggcggcgtca tcgtcgatcg agtgccgaag cgcgcgctgt tggtgatcac gcaatcctgc
     2761 gccatgctgc tcgctttcat cctcgccctc ttatcttttt tgaatgtcgt gcaagtctgg
     2821 catatcgtcg cgctggcctg cggcttgggt atcgtgaatg cgttcgatgg accggggcgt
     2881 caagccttcg tggtggagat ggtcggtcga gatgatctgc ccaacgccac cgccgttaac
     2941 tcgatgatgt tcaatgccgc gcgcgtcatc gggccggcca tcggcggtat
//
LOCUS       DASRTJ010000159         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117430,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000159 DASRTJ010000000
VERSION     DASRTJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117430"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFF70_03370"
     CDS             <1..358
                     /locus_tag="VFF70_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZY43766.1"
                     /translation="LIAVTADRSVGIDVEHVRVDFDFQPIVDRYFSAREKSELLGKPQ
                     SIHPQHFFNMWTRKEAILKMLGQGLSLLDQLAGTAEDGSLNSVGKGWTLYPVDVGAEY
                     EAALAVAGDRVQVIQQ"
     gene            complement(399..1520)
                     /locus_tag="VFF70_03375"
     CDS             complement(399..1520)
                     /locus_tag="VFF70_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZY43767.1"
                     /translation="MSSLKIDRRTFLKVAGATLTGAALGQPFSALAKPIVPPGEEKPK
                     PPFIDFQPSRVLASSVAIYDSLDAKHRKFLRSVPRDGVLSVAGEFEGPGQHNRTWYQT
                     RDGFAYSGWLQKMEPYRTPIVYTDIGEWGLWVEVIVPYLYAYAEPDERSKLDYKFYYG
                     TTYHVTATHTDAQGNVWYDTYDEFTSKDDDIKPTHHWILAKLVRKIDETIFTAINPHT
                     PEKHIYIDLARQTLSCQESGIVVLTSRIASGASFNVNGEVVDYSTPSGEYSAILKMPS
                     RHMRGPEVERNTGAWFDDPGVPWNTFFTYDGIAIHGTYWHNDFGVPRSHGCVNVPISV
                     AKFVYFWTNPVAPYEDDFVQGDVKTVNATPISVTSGKTE"
     gene            1758..2633
                     /locus_tag="VFF70_03380"
     CDS             1758..2633
                     /locus_tag="VFF70_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HZY43768.1"
                     /translation="MKTITWRKVFSTVFDYAQLTVGAGLIALGIDLFLVPNKIVSGGL
                     TGIATILHLTLGSPVGVVVLVLNIPLFIAGARWTGGLRFAMRTIYATAMMSILTDTLA
                     TIVATIPPITQPLLFTLYGGLLDGVGMGLVFRAQGTTGGTDIIARLANHFRGVPLGQT
                     MLAVNGLVLVTAAIVFGVEPALYALIVTFVATRVVDIVQGEAAYARAAIIISAKADDI
                     RSRVLADLERGVTILAGRGGYSEEGQEVLYCVVTRAEVSILKRLVQTIDPKAFVVITE
                     ASEVLGVGFKELRAS"
     gene            complement(2846..3217)
                     /locus_tag="VFF70_03385"
     CDS             complement(2846..3217)
                     /locus_tag="VFF70_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY43769.1"
                     /translation="MELGAFSVSLAVKDIETSKLFYEKLGFTVFAGDQSQNWLIMKNG
                     DHAIGLFQGMFDKNILTFNPGWNQDAKPLGKFTDVRELQRQLKNRGVNMVSEADESSA
                     GPASFMIVDPDGNTILVDQHV"
BASE COUNT          736 a          853 c          940 g          870 t
ORIGIN      
        1 cttgattgcc gtcaccgcgg atcgctcagt cggcatcgat gtggaacacg tccgtgtcga
       61 ctttgatttc caacccattg tcgatcggta tttttcggcg cgtgaaaaat ctgagttgct
      121 cggcaagccg cagagcattc atccgcaaca cttcttcaat atgtggacgc gcaaagaggc
      181 aatattaaaa atgttagggc aggggctgtc gctgttggat caattagcgg gaacggctga
      241 agatggctcg ctcaactcag tggggaaagg ctggacgttg tatcccgtcg atgttggcgc
      301 ggagtatgag gcggcgttgg ctgtggcggg cgatcgcgtt caggtgatcc agcagtaatt
      361 tgatatatag cggcgggcag tgttgcccgc cgcttgcgtt actctgtctt gccgctggtc
      421 accgagatcg gtgtagcgtt caccgtttta acatcgcctt gcacgaaatc atcctcgtat
      481 ggcgcgacag gatttgtcca gaaataaaca aacttcgcga ctgagatcgg cacattcacg
      541 cagccgtgac tgcgcggcac gccgaagtca ttgtgccaat acgtgccatg aatggcgatg
      601 ccgtcgtaag tgaagaatgt attccacggc acgccaggat cgtcaaacca cgcgccggta
      661 ttgcgttcta cttccggccc gcgcatgtgg cggctgggca ttttgagaat tgccgagtat
      721 tcaccgctgg gcgtgctgta atccaccact tcgccgttga cattgaacga cgcgccgctg
      781 gcgatgcgcg aagtcagcac gacaatccca ctttcttggc acgacaatgt ttgcctggcg
      841 agatcgatgt agatgtgttt ttccggcgta tggggattga tggcagtgaa tatagtctca
      901 tcgatcttgc gtacgagctt ggctaaaatc cagtggtggg tgggtttgat gtcgtcgtct
      961 ttggaggtga actcgtcgta cgtgtcatac cacacgttac cctgtgcatc ggtgtgtgtg
     1021 gctgtcacgt gataggttgt gccatagtaa aacttgtaat cgagtttaga tcgctcgtcg
     1081 ggttcggcat aagcgtaaag gtatggcacg atcacttcaa cccacaaacc ccattcgccg
     1141 atatcggtat aaacaatcgg cgtgcgatag ggctccattt tttgcagcca gccggaatag
     1201 gcgaagccgt ctcgcgtttg ataccacgtc cgattgtgct gcccgggtcc ttcaaattct
     1261 ccggccacgc tgaggacgcc atcgcgcggc acagatcgaa ggaatttgcg atgcttggca
     1321 tctaaactat catatatcgc tacactgctt gccagcacgc ggctcggctg aaagtcaatg
     1381 aaaggcggct tgggtttttc ttcgccggga ggaacaatcg gtttagctaa cgcgctaaaa
     1441 ggctgaccca gcgcggcgcc ggtcaaggtt gctccggcga ctttaagaaa tgtgcgtcga
     1501 tcgattttaa gagaagacat gatttgatta tacccaacgt tgtgattctc acaaactcca
     1561 ctctacctga tctgcggaat gctataatat tgccattttc atgggacgaa acagcttgat
     1621 atgttcttca aaggtggaac attttgctgg tcgatgatga accggttgca tcaccggata
     1681 caacgtcgag tgggaatgac cattcttgtg aaagaggggc gcgttgatcg cgcttctctg
     1741 tttaagagga gagaaagatg aaaacaatca catggcgaaa agttttcagc actgtctttg
     1801 attacgcgca gctgaccgtt ggtgccggtc tcatcgcctt aggcatcgat ctgtttttag
     1861 tgccgaataa aattgtatcg ggcggactga cgggcatcgc taccattttg catctgacgc
     1921 tcggctcgcc ggtcggtgtt gttgtgctgg tcttgaacat tccattgttc atagccgggg
     1981 cgcgttggac cggcggcttg cgttttgcta tgcgcacgat ttatgccaca gcgatgatgt
     2041 cgatcttgac ggacacgctt gcgacgatcg ttgcgacgat cccgccgatc acgcaaccac
     2101 tgctcttcac tttgtacggc ggcttgctcg acggagtcgg catgggcctg gtctttcgcg
     2161 cccaaggcac gacgggcgga acggacatca ttgcccggct ggcgaatcac tttcgcggcg
     2221 tgccactcgg tcaaacgatg ttggccgtga atggtttggt gttagtgacc gccgcgatcg
     2281 tctttggcgt cgaaccggca ttgtatgcgt tgatcgtcac ctttgtagcg acgcgcgtgg
     2341 tcgatatcgt gcagggtgag gcggcttatg cccgcgccgc gatcatcatc agcgcgaaag
     2401 ccgacgacat ccgatcgcgt gtcttggccg atctcgaacg gggggtcaca attctagccg
     2461 ggcgcggcgg ttattccgaa gaggggcagg aagtcttata ctgcgtcgtg actcgagcgg
     2521 aggtcagtat tctcaaacgg ttggtgcaga cgatcgatcc gaaagcattt gtagtcatca
     2581 ccgaggcgag cgaagtcctc ggcgtgggct ttaaagagtt gcgtgcgagt taaacactgg
     2641 gttgctcact cacgctgtgc tgtgcaggag tctaagctta gggatcagaa ataatgaacg
     2701 cgtcggatgg atcagatgat ctatcgggag cagtcttttg ttatgcagct cagcgctcgc
     2761 cttcagcggc gcgccgtcag gcgcgtccgc cgcaaggcgt tgttatgcgt cgcttcgcaa
     2821 atagaaatac caacttcaac attgcctata cgtgttgatc gaccagaatc gtgtttccat
     2881 ctggatcaac aatcataaag ctagccgggc cagcagagct ttcgtctgct tctgaaacca
     2941 tgtttacgcc acgatttttg agctggcgct gcaactctcg aacgtccgtg aatttcccga
     3001 gtggcttggc atcctgattc cagcccgggt tgaaggtgag aatgttctta tcgaacatgc
     3061 cttgaaacaa cccaatggcg tggtcgccat tcttcataat cagccaattc tgcgattggt
     3121 ctcctgcgaa aactgtaaaa cccagctttt cgtaaaagag cttcgacgtc tcgatatctt
     3181 ttacagctaa gctaacagag aaagctccca gttccatcat tgcctcctac gttgtgttcc
     3241 tgacgcatag tggttgttag atagcgttgt atcgcaccat acatgctatt ctttgaattt
     3301 caatttaccc agcaaaactt gaaaatgtag gtcggaacga acttcgcgtc cgtctttact
     3361 gcttgctcac aaaatctgat cgcttcgcgc gcattagcc
//
LOCUS       DASRTJ010000160         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117449,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000160 DASRTJ010000000
VERSION     DASRTJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117449"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFF70_03390"
     CDS             <1..992
                     /locus_tag="VFF70_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZY43770.1"
                     /translation="PAMLVYLDNATSQAHAPNENYAREVMELHTLGVAGGYNQQDVHE
                     VARALTGWSVAGERDAARVSRDQIGQFLFRPFMHDNDVKTILGMPFPAGGGESDGDKV
                     IELFASHPSTAKFIAKKLCVRFVSDTPPPSILDRAAATYTQTDGDIKAILKTILTSAE
                     FAQSIGQKYKRPFDFIVASLRALNAEVTLDPNVNLKAATKNNPNQALQRNPIFLQLVQ
                     LGHVPYLWSTPDGFPDKAVSWLSTSGTLGRWNFALSLVAGMRGVTIDDSSFTGISKSI
                     EESVDRLSTLLIGTPMPANPRSILIDYAQHAADQTRLPGLAALIISSPQFQIR"
     gene            1061..>2105
                     /locus_tag="VFF70_03395"
     CDS             1061..>2105
                     /locus_tag="VFF70_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZY43771.1"
                     /translation="MCEQCEQLTRRDFLKVSALTTLPLALPAWMPRLAFAPPGAEPRG
                     DVLVCIFQRGGMDGLNAVVPHAENAYHTARPTLAIPDPQSGRDNSVIDLDGFFGLHPS
                     LRSMKDIWDAKQLAIVHAIGSPNPSHSHFEAMDYLERGTPGNKRIPTGWLGRHLQITA
                     GQNNSPFRAVGFGSILQASLHGPIAATALQSIAEFHLQGQPEAIAQFQKSLAAMYASS
                     DAIAQEGQDTFDAMDLLVKSNPAQYQPPNGVTYPDGEYGLALMQVAQLITAEVGLEIA
                     CVDIGGWDTHANQGQLDGEMPGLLKEFSDGLAAFHADLQDRLNNVTVVTMSEFGRRVQ
                     ENASGGTDHGHGNV"
BASE COUNT          482 a          607 c          577 g          439 t
ORIGIN      
        1 gtccggcgat gttggtctat ctcgacaatg cgaccagtca ggcccacgcc cccaatgaaa
       61 attacgcacg tgaggtcatg gaattgcata cgctcggtgt ggccggcggt tacaatcaac
      121 aagatgtgca tgaagtcgcg cgcgcgttaa ccggctggag tgtggctggc gagcgcgatg
      181 cggcgcgtgt cagtcgagat cagatcgggc aattcttgtt tcggccgttt atgcacgaca
      241 atgacgtaaa aacaatctta ggcatgccgt ttccggccgg cggcggcgag agcgacggcg
      301 acaaagtgat tgagctgttc gcttcgcacc cgtccacggc gaaattcatc gcgaagaaat
      361 tgtgcgtgcg cttcgtgtcc gacactccgc cgccatccat tctcgatcga gcggcggcca
      421 cttacaccca gaccgacggc gatatcaaag cgatcctaaa aacgattctc acctccgctg
      481 agttcgcgca atcgatcggg cagaagtaca agcgtccgtt tgattttatc gtcgcgtcac
      541 tgcgggcgtt gaacgccgag gtgacacttg atcccaatgt caatctcaaa gcggccacca
      601 agaacaaccc caatcaagct ttacaacgca atcccatctt cttacagctc gtgcagttgg
      661 ggcacgtgcc ttacttgtgg tcaacgcctg atggttttcc cgacaaagca gtttcatggt
      721 tgagcaccag cggcacattg ggccgctgga atttcgcgct gagcctagtg gcaggcatgc
      781 gcggcgtgac gatcgatgac agcagcttca ccggcataag caaatcgatc gaggaatcag
      841 tggatcgcct gtccacgctc ttgatcggca cgccgatgcc ggctaacccg cgatcgatcc
      901 tgatcgacta cgctcagcac gcagccgatc aaactcgact gcccggtcta gcagcattga
      961 tcatcagctc gccgcaattt caaattcggt aaacgtagag aagtttcggt aaaatgtctc
     1021 cgagacaatc cgccgggtcg tctccaccag aggagaaaac atgtgtgaac aatgtgaaca
     1081 actcacccgc cgcgactttc taaaagtatc ggccctgacc acgctgccgc tggcgttacc
     1141 cgcttggatg ccccgactcg ccttcgcgcc gccgggagca gaaccgcgtg gtgatgtgtt
     1201 agtctgtatc tttcaacgcg gcggcatgga tggactcaac gccgtcgtgc cgcatgctga
     1261 aaatgcctat cacaccgcac ggccgacgct ggcaattccc gatccgcaat ccggccgcga
     1321 caacagcgta atcgatctcg acggtttctt tggcttacat ccctcattgc gatcgatgaa
     1381 ggatatttgg gacgcgaagc agttggcgat cgtgcacgcc atcggctcac ccaatccttc
     1441 gcattcacat ttcgaggcaa tggattattt ggaacgcggc acccccggca ataaacggat
     1501 tccaacgggc tggctgggtc gtcatctgca aatcaccgcc ggacaaaaca attcgccgtt
     1561 tcgtgccgta ggctttggat cgatcctgca agcttcactg cacgggccga tagcggccac
     1621 ggcgctccaa tcgatcgccg aatttcatct gcagggtcag ccagaggcca tcgcccaatt
     1681 ccaaaaatca cttgctgcga tgtatgccag cagtgatgcg attgcgcaag aagggcaaga
     1741 cactttcgat gcaatggatc tgttggtgaa gtccaacccg gcgcaatatc agcccccaaa
     1801 cggcgtgaca tatcctgatg gtgaatacgg cctagccctg atgcaagtgg cgcagttgat
     1861 cacagccgag gtgggcttgg aaatcgcttg tgtggatatt ggcggctggg atacccacgc
     1921 caatcaaggc cagctcgatg gcgaaatgcc cggcttgtta aaggaattca gtgacggact
     1981 ggccgccttc catgccgact tgcaagatcg attgaataac gtcacggtcg ttacgatgtc
     2041 ggagtttggc cgacgcgtgc aagagaacgc cagcggcgga accgatcatg gccacggcaa
     2101 tgtga
//
LOCUS       DASRTJ010000161         5666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117470,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000161 DASRTJ010000000
VERSION     DASRTJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117470"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFF70_03400"
     CDS             <1..340
                     /locus_tag="VFF70_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZY43772.1"
                     /translation="NMGGEPSGELGDALKSTFGSFAAFKENLNNAGVGRFGSGWAWLV
                     FDKNGKLAITSTPNQDNPLSDGLRPIMGVDVWEHAYYLKYQNRRADYLAAWWNVVNWT
                     EVAARFKKMK"
     gene            404..610
                     /locus_tag="VFF70_03405"
                     /pseudo
     CDS             404..610
                     /locus_tag="VFF70_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            1159..2616
                     /locus_tag="VFF70_03410"
     CDS             1159..2616
                     /locus_tag="VFF70_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZY43773.1"
                     /translation="MADQFRTEKDSLGEVQVPIEAYYGAQTKRAINNFHISGLRPWRA
                     FIWSIATIKRAAAEVNRDLGLLDPKIATAISAAAQEVIDGKWDDQFRVDPFQAGAGTS
                     HNMNANEVIASRAIEMLGGKRGDSTLVSPNDHVNMAQSTNDTIPTAIRLGVLWRLDEM
                     LGAIDKLAEALEVKAKEFDSIVKSGRTHLQDAVPVRLGQEFGAYAKEVRRDREKIAFA
                     ADGLRRLGIGGTATGTALNAHPEYHARMIKKLSELTGLMLYESDNLFESMQAHSDAVF
                     FSGAVRTLAQDLIRIANDFRLLSSGPSTGLDEIRLPAVQPGSSIMPGKVNPVLAEMLD
                     MAMFHVLGNDLTVTLAAQAGQLELNVMMPIIAHDLFEMMQVAIGSISAFTEYCVVGLK
                     ANPEKATGWLAKNAILVTALNPLIGYSKGAEIAKAAMARNVTIREVALEKIEVGELVN
                     KDNGMPVSIEEIDAILGDIRALTDGGIHGAGGGGG"
     gene            2723..3469
                     /locus_tag="VFF70_03415"
     CDS             2723..3469
                     /locus_tag="VFF70_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY43774.1"
                     /translation="MLRSNSCLVLIAVFISGCALSNPAPSSTATLAATPTSISSHPQK
                     ISFKTDDGLTLAGTLYGRGSNVVILSNVGDGTQPDWQELPDELARRGYLVLTYDWRGL
                     GLSAGKRDYVQSPLDLKAAIAFVRSRGALKTILAGASLGGMASLKIAAIEELVGVIVI
                     SSPRQAPDFVIDEKEIAAISVPKLFIGSQQDATVIYAETIAMHQIAIEPKLLKSYAGS
                     AHGTDILMTSDHADFVKTIIDFITTTLPNS"
     gene            3485..3943
                     /locus_tag="VFF70_03420"
     CDS             3485..3943
                     /locus_tag="VFF70_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY43775.1"
                     /translation="MLPILDDYLDRLATVHTDLTATLRDLPVEALDWSPGPEINSLAV
                     LAAHIAGSERYWIGEAAGQEPIKRDRAAEFKTAGVEADALIMRLDASLTHSQSVLTRL
                     TLADLEKITNVKHDDRSVTGAWALQHALEHVSVHLGHMQIMRQLWDQAHP"
     gene            4142..>5666
                     /locus_tag="VFF70_03425"
     CDS             4142..>5666
                     /locus_tag="VFF70_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZY43776.1"
                     /translation="MSKTSSGQPHPKRTSSAKKKPASLSADLVVANRKLRRESADRKR
                     ADEQLRLLSKAIEQSQSTVVITDPNGNIEYVNPKFVQLTGYSVEEALGQNPRIVKSGE
                     TSPEEYTRLWATITAGQTWHGILHNKKKSGELYWEETNISPVFDADGKITHFVAVKED
                     ITERKQAEQTLRESEERIRSILDTTVSGIITINERGTVESINCSASSMFGYAEIEVIG
                     QNIKLLMPNPYHTEHDGYIDNYLRTGTKKIIGIGREVTGLRKNGTTFPLSLAVSEVRV
                     GDLHLFTGIVQDITDSKQAEQLVEREKQKYEELFNTAQRQTQEMALLNEVQTALAREV
                     DLSDLIRTVVEAIAKAFGYTQVSVYLRQNDVLHMHYQVGYEHPLLEISMTQGVMARSV
                     RTSSVIFLPDVRSDPDFIGASVGINSEICVPLFDQQQAVGVLNVESTIGNLLTEADVR
                     LISVLGQQVNIALGRARLVGELRRQAEELHARNTDLDTFAHTVAHDLKSPIGAIIGFS
                     "
BASE COUNT         1427 a         1472 c         1562 g         1205 t
ORIGIN      
        1 gaatatgggt ggcgaaccga gcggtgaatt gggcgatgca ctcaaatcga ccttcggatc
       61 attcgcggcg ttcaaagaaa atttaaataa cgcgggtgtg ggccgcttcg gcagcggctg
      121 ggcgtggctg gtgtttgata agaacggcaa gctggcgatc acgagcacac cgaatcaaga
      181 caatcccctg tcggacggtc tgcgaccgat catgggcgtg gatgtgtggg aacacgcgta
      241 ttatctgaaa tatcaaaatc gccgcgccga ttatttagcc gcgtggtgga acgtagtgaa
      301 ttggactgaa gttgcggctc gtttcaagaa gatgaagtag agccatacat cgccctgtct
      361 gctgctcaac cattaaaaca aatttctttt tggagaaacg ataatgaccc atacaattac
      421 tgctttgtgc ttgcgcgaca gcgcatgcgt ggatgtctgc ccggtggagt gcatcattga
      481 gggcaaaccc gaagagcaat ggccgtggtt tatgatcgat cccgaaacct gcatcgattg
      541 cggcgcgtgc attccagagt gtccgtacga agcgatattc cctgaagacg aagtgccgac
      601 ggcgtttaag gccaagggcg gcgaatggct ggtcgacacg aaggccaatc tggccgacgg
      661 cgcgccgttc gaaggcgagg tcagcggcca tcacgttaaa gtgatgaacg cgcggcagat
      721 ggctgcgggc gaagtgttgg acttgacacc ggccacaccg atcaatgcgg ccttctataa
      781 agaagggccg ggctataaag ccaaggatat gtaagcgcag cacacagcgt gttgagtata
      841 ctcacggggc aatcgcactt tagtgttgcg attgcctcat tttttgtgag aagagaaatt
      901 caaaccgcga ccctgcacac aacaagcagg gcgagcgacg ccaagtgcgc gaagattatc
      961 aataacttaa cctgcgcgcc gtcacacccg cggaggcggg tgtccagtta ttcactctgg
     1021 attcccgcct tcgcgagaat gacaaccacc ttcatctatg taacgtgagt gaataataaa
     1081 gaacttggcg ttcttcgcgc cttagcggtt caatcttgac caaacctaaa aagtaatgat
     1141 cgtcgaggag aaaacttcat ggccgatcaa ttccgcactg agaaagattc gctaggcgaa
     1201 gtgcaagttc cgatcgaagc gtattacggc gcgcagacca aaagagcgat caataacttt
     1261 cacatcagcg gcttgcgccc gtggcgtgcc ttcatctgga gcatcgccac gatcaaacgc
     1321 gcagccgccg aggtcaatcg ggatttgggg ctgctcgatc cgaagatcgc cactgcgatc
     1381 agcgccgccg cgcaggaagt gatcgatggc aagtgggacg atcaattccg cgtcgatcca
     1441 tttcaagcgg gcgcgggcac cagccacaac atgaacgcga acgaagtgat cgccagccgc
     1501 gcaatcgaaa tgttgggcgg caaacgcggc gatagcacac tggtcagccc gaacgatcac
     1561 gtcaacatgg cgcaatcgac caacgacacg atcccaaccg cgatccgatt gggcgtgctg
     1621 tggcgcttgg acgaaatgct cggcgcgatc gataaattgg ctgaagcgtt agaagtaaag
     1681 gccaaagaat ttgattcgat tgtaaagtca ggtcgcacgc acttgcaaga tgccgtgccc
     1741 gtccgattgg gccaagagtt cggcgcctat gccaaagaag tgcggcgcga tcgagagaag
     1801 atcgcatttg cggcggatgg cctgcgccga ttgggtatcg gcggcacagc gaccggcacg
     1861 gcgctgaacg ctcatccgga atatcacgcg cggatgatca agaaattgag cgaactcacg
     1921 ggtttgatgt tgtacgaatc cgacaatttg tttgaatcga tgcaggcgca ttcggatgcg
     1981 gtcttctttt cgggagcagt ccgcacgctg gcgcaggatt tgatccgcat tgcgaatgat
     2041 tttcgattgt tgtcctccgg tccatcaacc ggcctggatg aaattcgatt gcccgccgtg
     2101 cagcccggct catcgatcat gcccggcaaa gtcaatccgg ttctcgcaga aatgttggac
     2161 atggcaatgt ttcatgtgct cggcaacgat ctcacggtga cgctcgccgc gcaagcgggc
     2221 caactcgaat tgaatgtgat gatgccgatc atcgcgcacg atctgttcga gatgatgcag
     2281 gtcgcgatcg gatcgatcag cgccttcacg gaatactgcg tcgtcggctt gaaagccaat
     2341 ccagaaaaag cgacgggttg gctggcgaag aatgcaatcc tcgtgacggc gttgaatcca
     2401 ttgattggct attcgaaagg cgcggagatc gccaaagcgg cgatggcgcg caatgtgacg
     2461 atccgagagg tcgcactgga aaagatcgaa gtaggcgaat tggtcaacaa ggacaatggt
     2521 atgcctgtat cgatcgaaga gatcgatgcg atcctcggcg acattcgagc gctgaccgat
     2581 gggggcattc acggcgcggg cggcggtggc ggctaatgag ttagagatga taaggaggcg
     2641 gcttcagccc agccgctttt ttgaaccaga gcgcagtgcc tgaggtatta ttcgcaacgg
     2701 agtcaagcaa aggagatcaa ttatgttgcg ctctaactca tgtcttgtgc tgatcgcagt
     2761 attcatatca ggctgcgctc tatccaatcc tgctccatct tccacggcca cgcttgcggc
     2821 cacacccaca tcgatcagca gtcatccaca aaaaatttcc ttcaagaccg atgatggtct
     2881 cacgctggcc ggcacactgt atggccgcgg atcgaacgtg gtgatcttat ccaatgtcgg
     2941 ggacggcacg caacccgatt ggcaagagct gcctgacgaa ctcgctcgac gcggctactt
     3001 agtgctgacc tatgattggc gcggacttgg cttatcagct ggtaaacgcg attacgtgca
     3061 aagccccctc gatctcaaag ccgcgatcgc atttgtccga tcgcgcggtg cgttaaaaac
     3121 tattttggcc ggagcaagtt tgggcggcat ggcttcactc aagattgcag cgatcgaaga
     3181 attggtcggc gtgatcgtaa tcagttcgcc gcgccaagcg ccggactttg tgattgacga
     3241 gaaagagatc gccgcgatta gcgtgccgaa gttattcatc ggcagccagc aagatgccac
     3301 tgtcatctac gctgagacga tcgctatgca tcagatcgcg atcgaaccca agctgctaaa
     3361 atcatacgcg ggcagcgcgc acggcaccga catcttgatg accagcgatc acgctgactt
     3421 tgtaaaaacg atcatcgatt tcatcacgac gacattaccc aattcataat caggagacaa
     3481 ctgcatgctg ccgattttag acgactacct cgatcgatta gccaccgtcc acacagactt
     3541 gactgccaca ttgcgcgatc tacccgttga ggcactcgat tggtcgcccg gcccagagat
     3601 caactcgctc gccgttctgg ccgcgcatat tgccggatcg gaacgctatt ggatcggcga
     3661 ggccgccggg caagagccaa tcaaacgcga tcgagccgca gaattcaaga ccgccggagt
     3721 tgaggccgat gcattgatca tgcggcttga tgcgtctttg actcacagcc aaagtgtctt
     3781 gactcgcttg accttggccg atctggaaaa gatcacaaac gtgaagcacg acgatcgatc
     3841 ggtgaccggg gcgtgggcat tgcaacatgc actggagcat gtctcggtcc acttggggca
     3901 tatgcagatt atgcggcagt tgtgggatca ggcgcatcct taagcgcgag caaccatcgc
     3961 tctttgttct ggctacgcgt catagctctt gagagcgtac gatatactac gctcaatgat
     4021 taacgcccat cagattgagg ctcgtcgact tcattcgagt cgcctcgttt gtttttgatt
     4081 agccagctgt agcagttcaa gggtataata tggcatcaaa tactatcggc caacatttga
     4141 tatgtcaaaa acctcaagcg gccaacctca cccaaaacgc acatcatctg ccaagaaaaa
     4201 accggctagc ctttccgctg atctcgtcgt tgccaatcgt aaattacggc gagagagtgc
     4261 cgatcgcaaa cgtgcggacg agcaactgcg tctgttatcg aaggccattg agcagagtca
     4321 gtcgacggtg gtaatcaccg atcccaatgg caacatcgag tacgtcaatc ccaaatttgt
     4381 ccagctaacc ggctattccg tcgaagaagc gctagggcaa aatccgcgca tcgtgaaatc
     4441 gggcgaaaca tcccccgaag agtacacgcg cttatgggca accatcacag ccggccagac
     4501 ttggcatgga atactccaca acaaaaagaa aagtggcgaa ctctactggg aggaaacgaa
     4561 catctcgccc gtgttcgatg cagacggaaa gatcacacat tttgtcgcag tcaaagaaga
     4621 catcaccgaa cgcaagcagg ccgagcagac tttgcgggag agtgaagaga gaattcgctc
     4681 tatcctggat acgacggtca gtggcatcat caccattaac gaacgaggta ctgttgaaag
     4741 cattaactgt tccgccagct ccatgtttgg ttatgctgaa atcgaggtca tcggccagaa
     4801 catcaagctg ttaatgccaa acccctatca cacagaacac gatgggtata ttgataatta
     4861 tctgcgtacg ggtacgaaaa agatcattgg catcggtcgt gaggttaccg ggctaagaaa
     4921 aaatggcaca acctttccgc tgagcttggc cgtgagcgag gtgcgtgttg gggacttgca
     4981 tctcttcacc ggtattgtcc aggacatcac cgacagcaag caagctgaac aattggtaga
     5041 acgtgagaaa caaaaatatg aagagctgtt caacaccgcc cagcgccaga cacaagaaat
     5101 ggctttgctc aacgaggtcc aaaccgcgct cgcccgcgaa gttgatctgt ccgatttaat
     5161 tcgcaccgtc gtcgaagcga ttgctaaagc atttggctat acccaagtga gtgtttacct
     5221 gcggcagaat gatgtcctgc acatgcacta tcaagtcggt tatgagcatc ccctattgga
     5281 aatttcgatg acgcagggcg tgatggcccg cagcgtacga accagctcgg tgattttcct
     5341 gccggacgtc cgcagtgatc ccgattttat tggagccagc gtaggaatca attcggagat
     5401 ttgtgtcccg ttatttgatc agcagcaggc agtcggtgtg ctgaacgtag aaagcaccat
     5461 tggcaatctt ttaaccgaag cggacgtgcg gctgatcagt gtcttgggcc aacaagtgaa
     5521 catcgcgctt ggacgcgcac gtttggttgg cgagctgcga cgtcaagccg aggaacttca
     5581 cgcgcgcaat accgacctgg acactttcgc tcatacggtg gcacatgatc taaaaagccc
     5641 aatcggtgcc atcatcggct ttagtg
//
LOCUS       DASRTJ010000162         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_312081,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000162 DASRTJ010000000
VERSION     DASRTJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312081"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..735
                     /locus_tag="VFF70_03430"
     CDS             166..735
                     /locus_tag="VFF70_03430"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43777.1"
                     /translation="MEIEGERTLRQAGTAGDIAFIRSVDARFIGQGSETNLPISEHGF
                     TQIDPIEIRRRFDQVYARLYGRTYPESPIEFVNFRVRASLPVRLLELPRLAVRSDRRQ
                     TAVKGQRQAFSGIAHEFIPFTVYDRYQLAPGASFAGPAIIEERESTVIIGEDATASID
                     EFGFLWIEMQERSNVSTFQRSRTFANAKI"
     gene            722..>1717
                     /locus_tag="VFF70_03435"
     CDS             722..>1717
                     /locus_tag="VFF70_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZY43778.1"
                     /translation="MQKFDPITLEILWRRLISIVDEADAAVARTAFSSVLRDAHDYTC
                     MFTDRQGRELAQGTFATPGQSGAMALGIKNLVRKIPTDSFHAGDVYVTNDPWALAGHL
                     NDVCVLSPIFYQDRLVAFTACVLHHSDIGGRVASDNHDVFEEGLFIPLVKLYDAGQEN
                     EAVLEMIRWNVRTPDQVIGDLRSQIAANHVCAEQVVRMLSESHLESLDDLAEEIISRS
                     ERSIRAAIAKVPPGLYRAEGTIEQMAGRADLTIKCAIEISGSDLHVDLSGSSPQVDWG
                     GNVVYNFTYAYVHMAMKSIFDPDIPNNDGSAAPIRLTAPEGTVVNCKFPAAVAARM"
BASE COUNT          374 a          490 c          476 g          377 t
ORIGIN      
        1 ccatcgcgcg aaatattttt tcgatctccg taaagtccgc cgaagacacc gatgctttgt
       61 ggctgcgcac cagatcgaat gcgcgcggcg cagtgaaaaa tcccaacgcc gatccgacac
      121 cggcattcgg cggactttac agagatcgaa aaaatatttc gcgcgatgga gatcgaaggc
      181 gaacgcacgc tgcgccaagc gggcacggca ggggacatcg ccttcatccg atcggttgat
      241 gcgcgcttca tcggccaggg ttccgaaacc aatttaccga tcagcgaaca cggcttcacg
      301 cagatcgatc cgatcgaaat tcgccgccgc ttcgatcaag tttacgcgcg cttgtacggt
      361 cgcacctatc ccgaatcgcc gatcgaattt gtcaactttc gagtgcgtgc cagtttgccc
      421 gtgcgattgc tggagctgcc ccgattggcc gtgcgcagcg atcggaggca gactgctgtt
      481 aaaggtcagc gtcaggcgtt ctctggcatc gcgcacgaat tcattccctt caccgtttac
      541 gatcgatatc aactagcgcc cggcgcatca ttcgccgggc cagccattat cgaagagcgc
      601 gaatcgacgg tgatcatcgg cgaagatgcc actgcgtcga tcgatgagtt tggtttcttg
      661 tggattgaga tgcaagagcg ttccaacgtc tcaacattcc aacgttcaag gacattcgcc
      721 aatgcaaaaa tttgatccga tcacgctcga aattttgtgg cggcgtttga tctcgatcgt
      781 cgatgaagcc gatgcggctg ttgcgcgcac cgccttctcc agcgtgctgc gtgatgcgca
      841 cgattacacc tgcatgttca ccgatcggca ggggcgcgag ttggcgcagg gcacatttgc
      901 cacgcccggc caatcgggcg cgatggcctt gggtattaag aatctcgtgc ggaaaattcc
      961 aaccgattca tttcatgcgg gcgatgtgta tgtcactaac gatccatggg cgctggccgg
     1021 acacttgaac gatgtgtgcg tgctcagccc gatcttttat caagatcgat tagtcgcttt
     1081 cacggcctgc gtcctacatc actccgacat cggcggccgg gtggcgtctg ataatcacga
     1141 tgtctttgaa gaaggtttgt tcattccgct ggtgaaactt tacgatgcgg gccaagagaa
     1201 tgaagcggtg ctggagatga tccgctggaa cgttcgcacg cccgatcaag tcatcggtga
     1261 ccttcgatcg caaatcgctg cgaatcatgt ctgtgccgag caagtcgttc gtatgttaag
     1321 cgaatctcat ctggaaagtt tagatgatct cgccgaagaa attatctcgc gcagcgaacg
     1381 cagcatacgc gccgcgatcg caaaagtgcc gccgggcctg tatcgggctg aaggcacaat
     1441 cgagcaaatg gccggccgcg ccgatctcac catcaagtgc gcgatcgaaa tatccggcag
     1501 cgatctgcac gtcgatctct ccggctcatc gccgcaagtc gattggggcg gcaatgtcgt
     1561 ttacaacttc acctatgcct acgtccacat ggcgatgaag agtatcttcg atcccgacat
     1621 tcccaacaac gacggcagcg cggcgccgat tcggctcacc gcgcccgaag gcacggtggt
     1681 caattgcaaa tttccggcgg cggtcgcagc gcgcatg
//
LOCUS       DASRTJ010000163         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117494,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000163 DASRTJ010000000
VERSION     DASRTJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117494"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFF70_03440"
     CDS             complement(<1..755)
                     /locus_tag="VFF70_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY43779.1"
                     /translation="MQIDLELYREDIEIHEPPIRLSAIDVNPEAAQRTIVFVHGFGGR
                     AVQWEKQLRFFSDENRVIAIDLRGHGYSSAPDSSYSMDEVQADLDAALAKLNVPDKFV
                     LVGHSFGGALAATYAERHPQRVEKLILIATAGEFVLNGWARLAFKLPVSALNLIRPLI
                     GRSLFAPPHVLKSFHNNNMAPFNGWSLFRNITVPTLVITGDRDKVFAQTVFEEVARKI
                     PNAQHVTVPASAHMVILERAEAVNRAIQRFLIET"
     gene            complement(768..1007)
                     /locus_tag="VFF70_03445"
     CDS             complement(768..1007)
                     /locus_tag="VFF70_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZY43780.1"
                     /translation="MPKFPVDAPLARVIKSFQSLGFRLVREGNHIAMERQNPDGTKTP
                     LTLPNHQTIKSSTLRTILTQTGISRDDFLKAYEDA"
     gene            complement(1007..1234)
                     /locus_tag="VFF70_03450"
     CDS             complement(1007..1234)
                     /locus_tag="VFF70_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZY43781.1"
                     /translation="MVKQFKILVEKHPDGFVAYPLGIKGVVVGEGDTYEEALADVNSA
                     IQFHIETFGPEMLEDDQPTLEAFIAETRVSV"
     gene            complement(1288..1653)
                     /locus_tag="VFF70_03455"
     CDS             complement(1288..1653)
                     /locus_tag="VFF70_03455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZY43782.1"
                     /translation="MNRLFTSFKYGIAGIAHAIRTQANFRIHLSISFVVIIAGLLLRS
                     SNAEWAILVLTIMIVLAAELFNTSIEAALDRVSPEQHPLVKVAKDAAAGAVLITAIGA
                     VVIGVLIFGPKLLGLIANR"
     gene            complement(1725..2261)
                     /gene="ybeY"
                     /locus_tag="VFF70_03460"
     CDS             complement(1725..2261)
                     /gene="ybeY"
                     /locus_tag="VFF70_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZY43783.1"
                     /translation="MQEARGTGQKAKVNHASRITHVASRINTNISVRYEDKVKPALLK
                     EAAQAALDQQRVRGKVEITIAITGDAQLRALNLAFRKINKPTDVLSFGLEPAADAEPS
                     EERHYLGDVIISYPMARAQAKAGGHPIEAELELLVVHGVLHLLGHDHAKPKEKAKMWK
                     AQSRVLRKIGAAITEPTD"
     gene            complement(2266..4326)
                     /locus_tag="VFF70_03465"
     CDS             complement(2266..4326)
                     /locus_tag="VFF70_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZY43784.1"
                     /translation="MKDNLLANQPAAWRNIRVWILGALFALGTAAILIVPMLTAGQVS
                     LNAGDVAADDLRAPRSLTYESDVLTNQARDEAENNVPTQYDPLDPRVARQQIAHVRQV
                     IDFVRAERADSLAPPDQRRAAINAISNVTLTPDTIDQLLSMPDSGWQRISAEVIAVVD
                     LAMRSEIRDMNVNEVKARLPALVAIDLNEAQTALVSQIAQNFIVPNQARNDAATQQVR
                     LAARDKIEPRVRQIEALDQLSLRQSSIGWGDIGGRLIISIVSALLMGLYLWRFEPDLV
                     ARPRNLFLLLLLLITFLILAKLIVPNRTVLPFLYPSAALVMLLTVLLGPGLALTSTVL
                     LAGLIGIMANGSMEITTYVAAGGVVATLALGRVERLNAFFLTGLYVALVNIAIILAFR
                     LPGGSTDPLGLLTLLAAAAINGGLSASLTLAGFFVVGNLFDITTTLQLLELARPTQPL
                     LNDLLHKSPGTYHHTLMVANLAEQAAERIGANALLTRVGAFYHDVGKTTRPYMFVENQ
                     VEGANVHDQLDPRTSAEIIVNHVKDGLELARRNRLPTRVRAFIPEHHGTMRVSFLYQK
                     AIESASNGKVDEAAFRYPGPKPQSKETALLMLADGCEAAVRATRPATPEEMNEIIRKV
                     IADRISWGQLDECPLTLADLDKVRQSFATTLQGLFHPRLRYPDRFIKPDGVVEQVRHA
                     EKEG"
     gene            4480..>5428
                     /locus_tag="VFF70_03470"
     CDS             4480..>5428
                     /locus_tag="VFF70_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZY43785.1"
                     /translation="MAAPNSASTAGTPRWLWFAFGGLLTITLLMVGLAVVLVIRSFSA
                     APAGIDTPTPIASAQPTSIVDQNQPQLIASPQTVTAGSPLAVFGSNWKSGEKVTIFLR
                     DPSKPTDPIMALGSGQASNDGTLAVTVAYPTDPRWAKLNKVDVIVQYATSGAYYVTSI
                     NVQSQIAMATSTAPPIPSATSTLIPPTKTPTRVPPTSTRVLPTATPVVITDWRGEYYP
                     NQTLTGAPAVVRNDKDVNFDWNVGSPFMFIPADYFSARWTRSLLFAAKTYRFTVRADD
                     GVRLWIDGSLIIEEWHSAAPSAYARDVTLGAGWHAIRLEY"
BASE COUNT         1191 a         1488 c         1503 g         1246 t
ORIGIN      
        1 gtctcgatca agaaacgctg aatcgcgcga ttgaccgcct cggcgcgttc aaggatgacc
       61 atgtgcgcgc tggccggaac cgtgacgtgc tgcgcgttgg gaattttcct ggcaacttct
      121 tcgaagaccg tttgcgcaaa aactttatcg cgatcgccgg tgatgaccaa cgtgggcaca
      181 gtgatgttgc ggaacaagct ccagccgttg aagggtgcca tgttgttgtt gtgaaaagat
      241 tttaagacat gcggcggcgc aaagagcgat cgaccgatga gcgggcgaat gagattcaag
      301 gcggagaccg gcagtttgaa agcgaggcgt gcccacccat tgagcacaaa ctctccggcg
      361 gtggcgatga ggatcagttt ttcaacgcgc tgcggatggc gctcggcata ggtcgcggca
      421 agcgcgccgc cgaatgaatg gccgaccaac acaaatttat ccggcacatt cagcttggct
      481 aaggccgcat cgagatcggc ttgcacttcg tccatcgaat aactggaatc gggcgcgctg
      541 ctgtagccgt gtccgcgcag atcgatcgcg atgacgcggt tctcgtcgga gaagaagcgc
      601 agctgcttct cccactgcac cgcgcggcca ccgaagccgt gcacaaacac gatcgtgcgc
      661 tgcgccgctt cggggttcac atcgatcgcg ctcaagcgaa tgggtggctc atgaatctcg
      721 atgtcttcgc gatagagttc aagatcgatt tgcatgatct ttatacctca tgcgtcttcg
      781 tatgctttta agaaatcgtc tcttgaaatc ccagtttggg tgagaatagt gcggagagtt
      841 gaacttttaa tcgtctggtg attgggcaga gtgagtggtg tctttgttcc gtctgggttt
      901 tgccgctcca tagcaatatg attgccttcg cgcaccaatc ggaaacctag actttgaaaa
      961 gatttgatta ctcttgccag cggggcatca actgggaatt ttggcattaa acgctgaccc
     1021 gcgtttcggc aatgaatgcc tcaagcgttg gttgatcgtc ctcaagcatt tctggcccaa
     1081 aggtctcgat atgaaattga attgctgaat tgacgtctgc caaagcctct tcgtaggtgt
     1141 caccttcgcc aacaacaaca ccttttatac cgagaggata agccacgaag ccgtcaggat
     1201 gtttttcgac aaggatcttg aattgcttga ccatattatc ttccctctca ccaagcgttt
     1261 tattgatcta agtatagcac gattgtgtta gcgatttgca atcaagccca acaactttgg
     1321 cccaaaaatc aacacgccga tgacgaccgc gccgatggcc gtgatcaata cagcgcccgc
     1381 cgcggcgtct ttcgcgactt taacgagagg atgttgctcg gggctgacgc gatccaacgc
     1441 cgcttcaatc gaagtgttga acaactcagc ggccagaacg atcattatgg tcagcaccag
     1501 gatcgcccat tcggcgttgc tgctgcgcag taataatccc gcgatgatga cgacgaatga
     1561 gatcgataaa tgaattcgga agtttgcctg agtgcgaatc gcgtgtgcga tcccggcgat
     1621 gccatacttg aaggaagtga atagtcggtt catgataggg gatgagggcc acgggctgaa
     1681 ggatgaatga gccaagatgc aagttgcatg ttgaattcat cttgtcagtc agttggctct
     1741 gtaatcgccg caccgatctt tcgcaacact ctcgattgcg ccttccacat cttggctttc
     1801 tcttttggct tggcatgatc gtggccgagc aaatgcagca cgccatgcac caccagcaat
     1861 tccagctcag cctcgatcgg atgaccgcca gctttggctt gggcgcgggc catcgggtac
     1921 gagatgatca cgtcgccgag gtagtgacgc tcttcagacg gttctgcatc tgccgccggt
     1981 tcaagtccga acgacaacac atccgtcggt ttgttgatct tgcggaaggc gagattgagc
     2041 gcgcgcagct gcgcatcgcc ggtgatggca atggtaatct ccactttgcc gcgcacgcgc
     2101 tgctgatcga gtgcagcttg agccgcttct ttgagcaggg cgggtttcac tttgtcttca
     2161 tagcgcactg agatgttggt gttaatgcgt gatgcaacgt gcgtgatgcg tgatgcgtga
     2221 ttaacttttg ccttttgccc tgttcctctt gcttcttgca tagagctatc cttctttctc
     2281 ggcatgccgc acctgttcca ccacgccgtc gggcttgata aatcgatcgg gataacgcaa
     2341 gcgcggatgg aacaagccct gcagcgtcgt cgcaaatgat tgccgcacct tatccagatc
     2401 ggccaaggtc aatggacatt catccaactg gccccagctt atccgatcgg cgatcacttt
     2461 gcggatgatt tcgttcattt cttccggcgt agccggacgc gtagcgcgca cggccgcttc
     2521 gcagccatcc gccaacatca acagcgccgt ctctttcgat tgcggcttcg ggcccggata
     2581 acggaaggcg gcttcatcga ctttgccatt cgaggcggac tcgatcgctt tctgatacag
     2641 gaaactcacg cgcatggtgc cgtgatgctc aggaatgaat gcgcggactc ttgtcggcag
     2701 ccgattgcgc ctcgctaatt ccaggccgtc tttcacatga ttgacgatga tctcggcact
     2761 ggtgcgcgga tcgagttgat cgtgcacatt agctccttcc acttgattct cgacgaacat
     2821 atacggccgc gtcgtcttgc cgacatcgtg atagaacgcg ccgacgcgcg tcagcagagc
     2881 attcgcaccg atgcgctcgg cggcttgttc cgccaggttg gcaaccatca aggtatgatg
     2941 atacgtgccg ggcgatttgt gcagcagatc gttaagcagc ggctgagtcg gtcgggctaa
     3001 ttccagtaac tgcaaagtag tggtgatatc aaagagattg ccgacgacga aaaatccagc
     3061 gagggtcagg ctggccgata agccgccatt gatcgccgca gcggcgagga gtgtcagcaa
     3121 ccccaacggg tcggtcgatc cgccgggcaa acgaaaggca agaatgatgg cgatgttgac
     3181 tagcgccaca tataagcccg tcaagaaaaa ggcattcaat ctttcgacgc gacctaatgc
     3241 cagagtggcg acgacaccgc ccgcggcgac ataggtggtg atctccatcg agccgttggc
     3301 catgatcccg atcagtccgg ccaacaacac ggttgatgtt aacgccaggc cgggacctaa
     3361 cagcacggtg agcagcatca ctaatgccgc cgacggatac aagaacggca gcaccgtccg
     3421 gtttggcacg atcaatttcg cgaggatcaa aaaagtgatc agcaataata agaggaggaa
     3481 taaattgcga ggccgcgcga ccagatcggg ttcaaagcgc cataagtata gacccatcaa
     3541 gagcgccgaa acgatcgaga tgatcaaccg gccgccgatg tcaccccagc cgatgctgga
     3601 ttgacgcagg ctcaactgat ccagcgcttc gatctgccgg acgcgcggtt cgattttatc
     3661 gcgggcagct aagcggactt gctgagtcgc cgcatcgttg cgagcttgat tcggcacaat
     3721 aaagttttgt gcaatctggc tgactaaagc agtctgcgct tcattcagat cgatcgcgac
     3781 tagagcgggc aggcgcgcct tgacttcatt cacattcata tcgcggattt cgctgcgcat
     3841 cgccagatcg accacggcga tcacttcagc cgaaatgcgc tgccaaccgc tatcgggcat
     3901 gctcaacaat tgatcgatcg tatcaggcgt gagggttaca ttactgatgg cattgatcgc
     3961 ggctcgacgt tgatcgggtg gggccaaact atcggcgcgt tcggcgcgca cgaaatcgat
     4021 tacctgccgc acatgcgcaa tctgctgccg cgccacacgt ggatcgagcg gatcgtattg
     4081 cgtgggcaca ttattctcgg cctcatcgcg cgcctgattg gtcagtacat cgctttcata
     4141 cgtcaaactg cgcggtgcac gcaggtcatc tgcggcgaca tcgcctgcgt tgaggctcac
     4201 ttgaccggca gtcaacatcg gcacgatcaa gatcgccgcg gttcccaagg cgaacaacgc
     4261 ccccaagatc cacacgcgga tgttgcgcca ggccgcaggt tgatttgcca gaaggttgtc
     4321 tttcaaaatt gccttcatca aacagggacg agagttacaa cagcatatcg gccgctgttc
     4381 gcagtcattg taccataggc ttcgggcaca ttttgcctgt ttcaaacgtc tctaaaggta
     4441 gtaatatttt gtttggatgc tgaaacagga ggacagacta tggctgcacc gaatagcgcc
     4501 tcaaccgccg gaaccccgcg ctggctgtgg ttcgcctttg gcggattgct caccatcacc
     4561 ttgctgatgg tcgggctggc ggttgtgctg gtcatccgat cgttctcggc ggccccagcg
     4621 ggtatcgata cgccgacgcc gatcgcttcg gcgcagccaa cttcgatcgt tgatcaaaac
     4681 caacctcagt tgattgcttc gcctcagacc gttacagcgg gatcgccgct ggctgtgttt
     4741 ggatcaaatt ggaagtcggg tgagaaagtc acgatcttcc tgcgcgatcc ttcaaagccc
     4801 accgatccta ttatggcttt aggttccggt caagcatcga acgatggaac tctcgccgtg
     4861 acggtagctt atcccaccga tccacgctgg gcaaagttga acaaagtcga tgtgatcgtg
     4921 caatacgcga cgagcggtgc ctactacgta acatcgatca acgtgcagtc gcaaatcgcc
     4981 atggcgactt cgaccgctcc accgattcca tctgcgactt caactttgat tccgccgacg
     5041 aaaactccga cgcgagttcc acccacctcg acgcgcgtgc tgccgacggc aacgccggtg
     5101 gtcattaccg attggcgcgg cgaatattat ccgaatcaaa cgttgaccgg cgctccggct
     5161 gtcgtgcgca atgacaaaga tgtgaacttc gactggaacg ttggctcgcc gttcatgttt
     5221 attccagctg attacttctc cgcgcgttgg actcgatcgc tcttgttcgc cgcgaagact
     5281 tatcggttta cagtaagagc tgatgatggc gtgcgcttgt ggatcgatgg cagcttgatt
     5341 atcgaagagt ggcattcggc cgcgccgtcc gcttacgcgc gtgatgtgac gttgggcgcg
     5401 ggctggcatg ccatccgcct cgaatact
//
LOCUS       DASRTJ010000164         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269036,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000164 DASRTJ010000000
VERSION     DASRTJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269036"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1434
                     /locus_tag="VFF70_03475"
     CDS             172..1434
                     /locus_tag="VFF70_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZY43786.1"
                     /translation="MPMTLAEKILAAHCDRSEVHPGQFISARVDIVLASELSGIVAIE
                     EFEKIKGARIFDPKKVVFVMDHFTPAKDIQSAEIVKRCREFAREHNITFFDVGRAGIQ
                     HVLLPEKGFISPGDIIAGADSHTCTHGFLGTFGTGIGSTDAAAAMALGEIWLKVPESI
                     KLIYHGQLPKWVGGKDAILQTISRLGVDGARYQAMEFVGEAIDHMGLSDRFTMANMAI
                     EAGAKTGLFAADDKARAFAEKTGAHPGTYHRSDPDAQYAATYEFDVDQFEPFVAVPYL
                     PENVKPVSELAGLEIHQVVIGMCTNGNIEDMRAAAEVLRGRKIHPRVRAVIIPGSQKV
                     YLDALHEGLIDIFVEAECSVSTPTCGPCLGGHMGVIASGERCVSTSNRNFKGRMGHAT
                     SEVYLANPYVAAASAIAGCLASPAEVMN"
     gene            1473..3572
                     /locus_tag="VFF70_03480"
     CDS             1473..3572
                     /locus_tag="VFF70_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZY43787.1"
                     /translation="MTYESFYHTTISDLLSMQAKQYADRVFIRLNDQTWTYTETNRQA
                     AALAAGLIDLGLRPGDRLAIILPNVPAYVITIFAAAKAGLILVPINIRRNKAEVQTRL
                     IKTDAVALITDRSAVGPGNIDHLTLARELQIDLPDLKHIIAINGDTGDAIAWKQLLAS
                     TAALPPSIVQPDDPAAIVHTLGNSGEPRGAVLTHGSLVHNAANIASTLACTPDDVFLG
                     AVPFSNSFGLAPTILACAIAGAQLALLPTYHPAEALRVIEQAKVTIHHGVPTMFALEL
                     NQPDFRSDACASLRTGVISGAHCPPDLATRVRDQMHCNISLAYGLTEASPAVTMTHLD
                     DGPITATQTVGRPLEGVTLKVIDADGSILPDGSEGELCVRGYNVMQGYWNDPKLTAQV
                     IDAEGWLHTGDLAVIDPDSPVRIVGRKGEVINRGGFKLYPGTIEMVLRSYPGVKEAAV
                     VGIPDVIFGELPIACVVRAPGADFKADQLLSFAGQNLTDYAVPDRVVFFEALPRRGSG
                     PVQKDILRERIRIRGHAWKFGKNIDTDAIIPARHCNTADPRELAQHCMEDADPDFVRK
                     MRRGDVIVADTNFGCGSSREVAPITIKAAGVSAVIAKSYARIFFRNSINIGLPILECE
                     SAVDGIAEGDEIEVEPATGTIRNLTRGETYRAEPFPDFLQRIIDRGGLLAYVEERLAA
                     EPFRRNVSTQDKIHESN"
     gene            3559..4095
                     /locus_tag="VFF70_03485"
     CDS             3559..4095
                     /locus_tag="VFF70_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZY43788.1"
                     /translation="MNPIKQLLQEHVEIMAQIADLRMAARDLTIRGDAALPESLPVLR
                     RIGHMMETQLALHARKEDEALFPAIEAALGADGSPTIPMRQEHKDIHAQGELMRQTLR
                     ELNEVQHPAIESGGARLRSLAADGDSAASLRATAEEIIDLLDAHFGKEEQMLFPMTES
                     LLDEGTLNAVGAKMEAMH"
     gene            4092..5240
                     /locus_tag="VFF70_03490"
     CDS             4092..5240
                     /locus_tag="VFF70_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY43789.1"
                     /translation="MIGFDLTDEQREFRDLAHKFAEKTIRPIAPEADEKEEVPWDVVK
                     KAHQAGLITVAFPEKYGGGGVESVLTKAIIDEEIFWGCAGIGTLIGGVGLCATPILLA
                     GTEEQKEKYITGFCDPNKVTLGAYALTEPAAGSDVASMTTSAHREKDKYFLNGSKTFI
                     TNAGIADIYLVFATIDPARGTDGITAFIVEKDWPGVTPGRKEKKMGIRASHTAGITFD
                     NVEVPIENRLGQEGEGFKIAMQTFDITRSHIAAGAVGIARAAYEYALGYAHERKQFGK
                     PIGSFQAIAFMLADMAMQIDAARLLTWRAAWLYDNRQSCTTEASMAKAFAADVAMKAT
                     TDAVQILGGYGYSREYPVEKWMRDAKIMQIYEGTAQIQRLVISRRLKT"
     gene            5320..>5729
                     /locus_tag="VFF70_03495"
     CDS             5320..>5729
                     /locus_tag="VFF70_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZY43790.1"
                     /translation="MKTYNIGVIAGDGIGPEVVNEGLKVLRAVKDGFDFNLVEYPYSG
                     AYYLKTKELVPDKVIDEWRTLDAVFLGAIGLPGVEPGLVERSVIMGLRMGLDLYVNLR
                     PVKLFAEHLCPLKDKTPTDVDFVVIRENTEDAYGG"
BASE COUNT         1303 a         1556 c         1697 g         1173 t
ORIGIN      
        1 gaattggctg atcattacga tcatatctgc ggtcaactct cacttatgat ggccgagccg
       61 gacaagtgga cgccgaaaca aagtggatca tgaaacgacg aaacgggaag taacgcttca
      121 cgctccacac ttcacgcctc acaacctacg ctttacgaat ggagataaca catgccaatg
      181 accttagcag aaaaaatttt ggcggcacac tgcgatcgat cggaagtcca tcccggccaa
      241 ttcatcagcg cgcgcgtgga catcgtcttg gcctcggaac tctcgggcat tgtggcgatc
      301 gaagagttcg agaagatcaa aggcgcgcgc atcttcgatc cgaagaaagt ggtcttcgtg
      361 atggatcact tcaccccggc caaagacatc cagagcgcgg agatcgtcaa gcgctgccgc
      421 gagtttgcac gcgaacacaa cattactttc ttcgatgtcg gccgcgccgg aattcaacac
      481 gtgctgctgc cggagaaagg tttcatctcg cccggcgaca tcatcgcggg cgcggattcg
      541 cacacgtgca cgcacggttt tctcggcacg ttcggcaccg gcatcggctc gaccgacgcg
      601 gcggcggcga tggcgctggg tgaaatctgg ttgaaagttc cggagagcat caaattgatc
      661 tatcacggcc aattgcccaa gtgggtcggc ggcaaagatg cgatcctgca aacgatcagc
      721 cggctcggcg tcgatggcgc gcgctatcaa gcgatggagt tcgtcggcga agcgatcgat
      781 cacatgggcc tctccgatcg gttcacgatg gcgaacatgg cgatcgaagc gggcgcgaag
      841 accggcctat tcgcggccga tgacaaagcg cgcgcgttcg cggaaaaaac cggcgcgcat
      901 cccggcacat atcatcgcag cgatcccgac gcgcagtatg cggccacgta tgaattcgac
      961 gtagaccagt tcgagccatt tgtcgccgtg ccgtatttgc cggagaacgt caagcccgtt
     1021 tcggaattag ccggacttga aattcatcaa gtcgtcattg gcatgtgcac caacggcaac
     1081 atagaagata tgcgtgccgc cgccgaagtg ctgcgcggcc gcaagattca tccgcgcgtg
     1141 cgtgccgtga tcattcccgg ctcgcaaaaa gtttatctcg atgcgctgca cgaagggttg
     1201 atcgacatct tcgtcgaggc ggaatgttcc gtcagcacgc cgacgtgcgg tccgtgcctg
     1261 ggcggacaca tgggcgtcat cgccagcggc gagcgctgcg tctcgaccag caatcgcaac
     1321 ttcaaaggcc gcatgggaca cgcgaccagc gaagtgtatt tggcgaatcc atatgtcgcg
     1381 gcggcttcgg cgattgcggg ttgtctcgca tcgccagcag aagtaatgaa ttgataagta
     1441 acgagtaatg agtaaccagt aaaggaacaa tcatgaccta cgaatctttt taccacacca
     1501 cgatcagcga tctgctcagc atgcaagcga agcaatatgc cgatcgcgtc ttcatccgat
     1561 taaacgatca aacttggact tacactgaaa ccaatcggca ggcggcggcc ctggcggccg
     1621 ggctgatcga tctgggtctg cgccccggtg atcgattggc gatcattttg cccaacgtgc
     1681 ccgcgtatgt gatcacgatc ttcgccgccg ctaaagcggg cttgatcttg gtgccgatca
     1741 atatccgccg caacaaagcc gaagtgcaaa cccgcttgat caaaacggac gcagtcgcgc
     1801 tgatcaccga tcggagtgct gtagggcccg gcaatatcga tcacctgaca ttggcacgcg
     1861 aattgcagat tgatctaccg gatctcaaac acattatcgc gatcaatggt gatactggcg
     1921 atgcgattgc atggaagcaa cttctcgcat caaccgccgc gctgccacca tcgatcgtcc
     1981 agcccgatga tccggcggcg attgtgcaca cgctcggcaa cagcggcgag ccgcgcggcg
     2041 cggtgttgac gcatggcagt ctcgtgcaca acgcggctaa tattgcatcg acgctggctt
     2101 gcacacccga tgatgttttt cttggcgcag tgcctttttc aaattcattc ggactcgcgc
     2161 cgacaattct ggcttgcgcg attgcgggcg cacagttggc attgctgccc acgtatcatc
     2221 ccgctgaagc gctgagagtg atcgagcaag cgaaagtcac cattcatcac ggcgtgccga
     2281 cgatgttcgc gttggaactc aatcagcctg attttcgatc ggatgcctgc gcgagtttgc
     2341 gcaccggcgt gatctccggc gcgcactgcc cgcccgatct tgcgacgcgc gtgcgcgatc
     2401 aaatgcactg caacatcagc ctggcgtatg gcttgacgga agcctcgccc gccgtcacga
     2461 tgacgcattt ggatgatggc ccgatcaccg cgacgcaaac agtcggtcgt ccgcttgaag
     2521 gcgtaacctt gaaagtgatt gacgccgatg gatcgatctt gcccgacggc tctgaaggtg
     2581 aactctgtgt gcgcggttac aacgtgatgc aaggttattg gaacgatccc aaactgactg
     2641 cacaagtgat cgatgccgaa ggttggctgc acacggggga tcttgctgtg atcgatcccg
     2701 atagcccggt gcgcatcgtc gggcgcaaag gtgaagtgat caatcgcgga ggctttaaac
     2761 tttatcccgg cacgatcgag atggtgttgc gatcgtatcc cggcgtgaaa gaggcggcgg
     2821 ttgtcggcat ccccgatgtg atctttggtg aactgccgat cgcctgtgtg gtgcgcgcgc
     2881 ccggcgctga tttcaaagcc gatcaactgt tatctttcgc tgggcaaaat ttaacggact
     2941 atgccgtgcc cgatcgcgtc gtgttcttcg aggcgctgcc gcgtcgaggc agcggcccgg
     3001 tgcagaaaga tattttgcgt gaacggattc gcattcgcgg ccacgcctgg aagtttggca
     3061 agaacatcga cacggatgcg atcattccgg cgcggcattg caacaccgcc gatccgcgtg
     3121 agttggcgca gcactgcatg gaagacgccg atcccgattt cgtgcgcaag atgcggcgcg
     3181 gcgatgtgat tgtggccgac acgaacttcg gctgcggctc atcgcgcgaa gtcgcgccga
     3241 tcacgatcaa ggccgcgggc gtctcggcgg tgatcgccaa atcatatgcg cgcatttttt
     3301 tccgcaattc gatcaacatc ggtctgccga tcctagaatg cgagtccgcc gtggacggca
     3361 tcgccgaagg tgacgagatc gaagtcgaac cggcgacggg caccatccgc aatttgacgc
     3421 gcggcgaaac ctatcgggct gaacccttcc ccgattttct gcagcgcatc atcgatcgcg
     3481 gcggactgct ggcgtatgtt gaagaaagat tagcggctga gccgttccgc cggaacgtct
     3541 ctacgcagga taaaattcat gaatccaatt aaacaacttt tacaagaaca cgtcgaaatc
     3601 atggcgcaga ttgccgattt gcgcatggcg gcacgcgatc ttacaatccg tggcgatgcg
     3661 gccttgcctg aatcactgcc agtgctgcgc cgcatcggcc acatgatgga aacacaattg
     3721 gctttgcatg cgcggaaaga agacgaggca ctctttcctg caattgaagc ggccttaggc
     3781 gcagacggtt cgccgacgat accgatgcgg caggagcata aagacattca cgcgcaaggc
     3841 gaattgatgc gccaaactct gcgcgaactc aacgaggtgc agcatccggc catcgagtcc
     3901 ggcggcgcgc gactgcgatc gctcgcggct gacggtgaca gcgcggccag cctgcgtgct
     3961 acagcggaag agatcattga tctactcgac gcccactttg gcaaagaaga gcagatgctc
     4021 tttcccatga cggagagcct gctggatgaa gggacgctca acgcagttgg cgcgaagatg
     4081 gaggcgatgc attgatcggt tttgatttga ccgacgaaca acgcgagttc cgtgatctcg
     4141 cgcataaatt tgccgagaaa accatccgac cgattgcgcc ggaggcggac gaaaaagaag
     4201 aagtaccatg ggatgtggta aagaaggcgc atcaagccgg attgatcacc gttgcctttc
     4261 ctgaaaaata cggcggcggc ggtgtggaga gcgtgctgac caaagcgatc atcgacgaag
     4321 aaattttctg gggctgcgcc ggtattggca cgctgatcgg cggcgtgggc ttatgcgcga
     4381 cgccgatttt gttagcaggc acggaagagc agaaggagaa atatatcacg ggcttctgcg
     4441 atccgaataa agtcaccttg ggcgcgtacg cgctgaccga accggccgcc ggttccgatg
     4501 tggcttcgat gacgacctcg gctcaccgcg aaaaagataa atactttctg aatggcagca
     4561 agaccttcat taccaacgca gggattgcgg acatctactt ggtcttcgcg acgatcgatc
     4621 cggcgcgtgg cacggacggc atcaccgcgt tcattgtcga gaaagattgg ccgggtgtaa
     4681 cacccggccg caaagagaag aagatgggca ttcgcgcctc gcacacggcc ggtatcacgt
     4741 tcgataacgt cgaagtgccg attgagaacc gcttgggtca agaaggtgaa ggctttaaga
     4801 tcgccatgca gacttttgat atcactcgat cgcacattgc cgctggcgct gtcggcatcg
     4861 cgcgtgcggc gtatgagtat gcgttggggt acgcgcatga gcgcaagcag tttggcaaac
     4921 cgatcggctc gtttcaagcg atcgcgttca tgctggccga tatggcgatg cagatcgatg
     4981 cggcgcgctt gctgacgtgg cgcgcggcat ggttgtatga caacagacaa tcgtgcacca
     5041 ctgaagcgag tatggcgaaa gcgtttgccg ctgatgttgc gatgaaagcc acgaccgatg
     5101 cggtgcagat cctcggcggc tacggttaca gccgcgagta tccggtggag aagtggatgc
     5161 gcgatgcgaa gatcatgcag atctatgaag gcacggccca gattcagcgg ctggtgattt
     5221 cgagaagatt aaaaacgtga tgcgtgacga gtgatacgtg aaggattatt tcacgcatga
     5281 cgtaatcacg catcacgatt tattcaacaa ggatgctcca tgaaaacata caacatcggc
     5341 gtcatcgcgg gcgatggcat tgggccggaa gtcgtgaacg aaggtctgaa agttttgcgc
     5401 gcggtgaaag atggcttcga cttcaatctc gtcgaatatc cgtatagcgg cgcgtattat
     5461 ctcaagacca aagaactggt accggataaa gtgatcgatg aatggcgaac gctcgatgcg
     5521 gtctttctcg gtgcgatcgg acttcccggc gttgaacctg gcctggtgga acgatcggtc
     5581 atcatgggct tgcggatggg acttgatctg tatgtgaatc tgcggccagt caaacttttt
     5641 gccgagcatc tgtgtccgct gaaagataag acgccgacgg atgtggactt tgtcgtgatc
     5701 cgcgagaaca ccgaagacgc ttatggcgg
//
LOCUS       DASRTJ010000165         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_9412,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000165 DASRTJ010000000
VERSION     DASRTJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9412"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..988)
                     /locus_tag="VFF70_03500"
     CDS             complement(29..988)
                     /locus_tag="VFF70_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43791.1"
                     /translation="MTNSDQARLTNNKIFLSGSAGTGKTTIAVDRLRSLIDSGISGNS
                     ILVLAPQRSLLKPYQQLLRRADLPAGEQIEALTIGGLARRSIDLMWPLVAPTGKFTRP
                     DQPPTFLTVETAQYYMQKVVEPLRAEKLFFDGVRVPGQRLYSQLLDNLNKAALVGFPY
                     TEIGKRLISAWEGPAAQTMTFAQVQDCIQRFRDYCLANNLLDFSLQIELFAGLSKTAG
                     FRDRLFKKYRHLIADNTEEDTPITHDLLRDWIPAADSALIIFDNEAGYRSFLGADPRS
                     AYKLRSVCDRSIDMTCDSVISIVRSHTDWSLYADRGSAPKNDR"
     gene            complement(1201..>2181)
                     /locus_tag="VFF70_03505"
     CDS             complement(1201..>2181)
                     /locus_tag="VFF70_03505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZY43792.1"
                     /translation="ALQLCPELIVLPGRHDLYSQHSHEVMQILYEITPLVEQISIDEA
                     FLDITGTELRYGLPERLARQLHDRVRDQFGLPCSIGVASNKLVAKIGTEKAKPNGILI
                     IPHGEEAKFIAPLPIRALWGVGPKTAAILQAAGIETIGQIAQTRSDVLARHLGSRGAA
                     DLLRRANGIDEGDIETDRITKQISQEETFARDVTDAKRLRAVILEQSEHVGAQLRAEK
                     FGAKTIAIKIRYKDFTTLTRQTTLPQPTDLDQPIFETAWKLFEANWTRRAVRLIGVAA
                     RGLGPVAQQLNLFEPHDNRSEKLTRTVDAIRQKFGESKLKRGSTLRPKKK"
BASE COUNT          489 a          565 c          583 g          544 t
ORIGIN      
        1 cgttgatcat ttttgataac gaagccggct atcgatcatt tttgggcgcg gatccccgat
       61 cggcatataa actccaatcg gtgtgcgatc gaacgatcga gatgactgag tcgcatgtca
      121 tgtcgatcga tcgatcgcac accgatcgaa gtttatatgc cgatcgggga tccgcgccca
      181 aaaacgatcg atagccggct tcgttatcaa aaatgatcaa cgctgaatca gcggcaggta
      241 tccaatcgcg tagcaagtca tgagtaatgg gtgtgtcttc ttcggtattg tccgcgatca
      301 agtgtcgata tttcttgaac aaccgatcgc gaaacccggc ggtcttcgat aacccggcga
      361 acagttcgat ctgtaacgag aagtcgagca agttgttggc caggcaataa tcgcggaagc
      421 gttggatgca atcttgcact tgagcaaagg tcatcgtttg cgccgctgga ccttcccacg
      481 cgctgatcaa gcgcttgccg atttcagtgt acggaaagcc aacgagcgct gccttgttga
      541 gattgtcgag cagttgactg tataggcgtt ggcccggcac gcgcacgcca tcgaagaaaa
      601 gtttttcggc gcgaagcggc tcgacgactt tctgcatgta gtattgcgcc gtttccaccg
      661 tcagaaatgt aggtggctga tctggccgtg taaacttgcc agtgggtgcg accagcggcc
      721 acatcagatc gatcgagcga cgtgccaaac caccgatggt cagcgcctcg atctgttcgc
      781 ctgcggggag atcagcacgg cgcagcaatt gttgataggg cttcaataaa ctgcgttgcg
      841 gcgcgagcac taaaattgaa ttaccgctga tgccagagtc gatcaatgag cgcaatcgat
      901 cgacggcgat tgtagtcttg cctgtgccag cacttcccga taaaaatatt ttattgttcg
      961 tcagtcgcgc ttgatcgctg ttagtcattt acaatgtgtt cgcgtagaaa catcaactat
     1021 gcatcagaat caacttgttg ttttcttgaa gaagcaagaa gctatgcgaa gtatagcata
     1081 cgccaagtca ttgcgagacg catcatcttg catcgcgctc ttcgactgca attcgcaaaa
     1141 agcccgaatt tccgctcaga atgcttacat tttctcatga tttaacgacg atctcacaag
     1201 tcattttttc ttgggtcgca gtgtcgagcc gcgcttcaat ttcgattcac cgaatttttg
     1261 ccggatggca tcgactgtgc gggtgagttt ctccgatcga ttatcatgcg gctcgaataa
     1321 gttcagttgc tgcgcgacag ggccgagtcc acgcgcggcg acgccgatca agcggacggc
     1381 gcggcgtgtc cagttggctt caaataactt ccaggccgtt tcgaagatcg gctggtcgag
     1441 atcagtcggt tggggcaggg tggtctgccg cgtaagagtg gtgaagtcct tgtagcgaat
     1501 cttgatcgca atcgtcttcg ctccaaattt ctccgcgcgc aattgtgcgc cgacatgttc
     1561 gctctgttcc agtatcacgg cgcgcaagcg tttagcgtcg gtgacgtcgc gcgcgaatgt
     1621 ttcttcctgg ctgatttgtt tggtgatgcg atcggtttcg atgtcccctt catcgatccc
     1681 gttggcgcgc cgcaataaat cagcagcgcc acgcgatccc aaatgccggg cgagtacatc
     1741 cgatcgagtt tgggcaatct gcccgatcgt ttcgatacca gctgcttgca agatcgctgc
     1801 ggtcttcggt cccacgcccc acaatgcgcg gatcggcagc ggcgcgataa acttggcctc
     1861 ttcaccgtga ggaatgatca agattccatt tggcttcgct ttttcggtgc caatcttcgc
     1921 cacgagtttg ttcgacgcca cgccgataga gcaggggagt ccaaattgat cacgcacccg
     1981 atcgtgcaat tgccgcgcta atctttccgg caggccatag cgcagctcag tgcccgtgat
     2041 gtcaagaaac gcttcatcga tcgagatttg ctcaacgagc ggcgttatct cataaaggat
     2101 ttgcatgact tcgtgagaat gttgcgaata caaatcgtgt cggccaggta acacaatcaa
     2161 ttcgggacac agttgcaaag c
//
LOCUS       DASRTJ010000166        15936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_193264,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000166 DASRTJ010000000
VERSION     DASRTJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193264"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFF70_03510"
     CDS             <1..309
                     /locus_tag="VFF70_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43793.1"
                     /translation="GGYDLNVESLDAIKSGAAQVTVGQNPYLQGYLPVLALVAQLRDG
                     KPPIKGWLNVGTEVVTKDNVDTLYKRESDPAYMTQWYADWIAKNFTDLNALQKPLPQH
                     "
     gene            425..1951
                     /locus_tag="VFF70_03515"
     CDS             425..1951
                     /locus_tag="VFF70_03515"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZY43794.1"
                     /translation="MAAQQEIILLENITKRFGGVTALDDVSFGIAPGEVHAVVGENGA
                     GKSTLMKILAGVHPQDNGKVILQGTPVVIRDPLDARSKGISIVFQELNLFPDLSASSN
                     IFVNRELSGRAGRLDQRAMDAATRQVFTAMELDIDPHVKVKKLSVGERQLVEIARTLQ
                     ARSEIIIMDEPNSALTQRETDRLFELIRHLRDQGITIIYVSHRLEEVFAIADRITVLR
                     DGHYQGTWRTTDTTIPKIVEAMIGRRLEEAFPDRPLVPAAAEVLLEVRDLTCGKTGPI
                     SFQVHAGEILGFAGLEGAGVEDIFRVLFGLDRVKSGEVIYAGQARRVRSPVEALKLGW
                     GLIPANRREQGLMINWPIRKNTTLVILDKLLNRLGLIDAAADRRTTQEYVERLNITTD
                     SIDKKVINLSGGNQQKVVVAKWLASGPKILILDDPTRGVDVGAKSEIYRLCDQLAQQG
                     LALLFTSSEVEEILGLCDRVLAFYKGQMLREFKRGEATKSDLMNVIASGANGNAVRTS
                     "
     gene            1932..2825
                     /locus_tag="VFF70_03520"
     CDS             1932..2825
                     /locus_tag="VFF70_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY43795.1"
                     /translation="MRSERVSFFSSGVPLTGILTLPDDPVGQQPAVVLAHGYANYKGE
                     FGGFDELADVLGRSGYVALQFDFRGCGETDAPVGKMLCATEWPLDVMSAVSYLQARSE
                     VDPQRIGLVGQSMGGGVVATVSALDDRVACTVSLASVSDGARWLHETWTQRRGASGWN
                     EFLIALQNDRHERALHGRSRFAPLPELLALTAEETATWLEMRARYPLFLYEAPWESID
                     DVLHFKPIDVVQHIQCPIRFVHGTADLMVDSSHSVDMHAHAGSQADLQLIDGADHALP
                     IGTHKGRVQELIAEWLDRYLK"
     gene            2870..3901
                     /locus_tag="VFF70_03525"
     CDS             2870..3901
                     /locus_tag="VFF70_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZY43796.1"
                     /translation="MKALVKERPEPGLSLLNIAEPKIANPDDVLFKVEYCAICVGETK
                     AYDWNEWAANDKTFKLPTVLGHEAVGVVVEVGSEVKQFKLGDRIVNDPLIYCGYCRQC
                     RSGFTNMCENREIYGKRRGAFAECAVLPERVLAHLPDKLSLAEGAVLENFGIAMHAVD
                     IEEHEPGEWAVVLGAGPIGILAAQILTAWGANVVITDLSDARLDFARQHTRAIAIDIK
                     QEDPVKKVLELTKGQGADFVIEMAANQAAMDQAFDMVRIRGTVVTMGSFNGPITFNPF
                     FKMTRREIKLQSMMGRTWESWRRMVQMIEKDQIDLKPLITHILPFEEYQRGFELVKGH
                     EVMKILLKP"
     gene            4037..5308
                     /locus_tag="VFF70_03530"
     CDS             4037..5308
                     /locus_tag="VFF70_03530"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZY43797.1"
                     /translation="MANFDQARTLLRQFKGDTYLHGLHVLPQVGSVTAQEGKRAVLVR
                     NTFTGSEVFTQTLKASLAAAGVQLLGEVEGAAPNAPREDLFRITTALIDLQPDVLVSF
                     GGGSTIDATKAAEVLRTLGGSIDDYFGTGLVTQALARGGKKLSPHVAIQTASSSAAHL
                     TKYSNITDVSTGQKKLIVDNAIVPPRPVFDYSVTFDAPISLTSDGALDGISHSLEVLY
                     SAVGKPEYATIEAVAAECIRLIVNYLPAVLNNPREPVGREALGLATDLGGYSIMLGGT
                     NGAHLTSFSLVDLMSHGRACGLLNPYYTVFFAPAVQAPLKVVGQIYRDAGYTQADIDQ
                     LSGRDLGVAVAEAMIAFEQKIGLPTTLNEVPGFSEAYIDRALTAARNPQLKMKLENMP
                     VKLNAETIDEYMGSVLQAAKTGDLKLIKNVT"
     gene            5374..6213
                     /locus_tag="VFF70_03535"
     CDS             5374..6213
                     /locus_tag="VFF70_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZY43798.1"
                     /translation="MKLGVNTYTYMWSIGFKGPNPAFPDNEARPERPLTALGLLEKAR
                     ALGLHLVQTGPNLPVDKLPEAEFEAFIQQARAWNIELELGTRGLDYDHLVKQVAMAKR
                     MGAKLIRTLPEIEGKYATDARLIPPVVRQIVPVLEREGVRLAIENGKTPAVNLRAALD
                     EIHSPCVGLVLDMVNSLAIPEGWRYVTQLLAPHTMCVHYKDFTIKRAWHMMGFICEGM
                     PAGKGLVETEWLLNELKASPYDFNVIIELWPPEQEKLQDTIDLEQAWAIESVPYLRKF
                     IKD"
     gene            6244..7512
                     /gene="larA"
                     /locus_tag="VFF70_03540"
     CDS             6244..7512
                     /gene="larA"
                     /locus_tag="VFF70_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZY43799.1"
                     /translation="MQIRLAYGKTGLMVELPDDRSVTVVEPQFVPGLPDAREALRQAL
                     RTPLNSTPLRELAKPTDKIGIVFSDITRPTPHHLLIPSVLEELAHVPRENITLFNALG
                     THRPNTEAELRGMLGDALVDGYRLVQNNSFDPATQVRVGVTSRGHEIWLNREVAGCDL
                     KVLTGFIEPHFFAGFSGGGKALMPGMAGQRTVLGNHDAGMIGNPQATWGITWGNPIWE
                     EVREVALQTERLFLFNVTLNKDKEITGVFAGDLDAAHAAGCAFVKQTAMVAVPRPFDI
                     VITTNSGYPLDLNLYQAVKGMSAAAQVVRPGGAIIVAADCWDGIPDHGLYGQLLREAS
                     SPQALLDRICAPGFLEQDQWQAQIQAIIQLKADVYVRTDNLTDAQIEAALLKPCHCIE
                     DTVAELLDRYGLEATICVLPEGSQTIPYIA"
     gene            7548..8438
                     /locus_tag="VFF70_03545"
     CDS             7548..8438
                     /locus_tag="VFF70_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZY43800.1"
                     /translation="MHYHSEELVKDLSVKAQRFRIHVLKMVYHAQTGHLGGAFSVAEI
                     VASLYFHHLQVDPAQPDWIDRDRLLFSKGHACAMLYTALAYRGFFPVEELATFRQFNS
                     RLQGHPDRLKTPGVEICAGPLGHGIAIGAGMALAQKMRGGKPSSLSAPSARASAGRVY
                     VVLGDGELDAGVIWEGAMTAAKYRLGNLTAILDYNGIQQTGATADVMPTEPIAAKWEA
                     FGWHVQEVHGHNVAEILAALDRADEVHARPSVIIARTTKGKGVSFMEYDHRWHGMPPN
                     REQYEKALAELEAGVQAWQN"
     gene            8426..9367
                     /locus_tag="VFF70_03550"
     CDS             8426..9367
                     /locus_tag="VFF70_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HZY43801.1"
                     /translation="MAELAPMRKAYGEALVKLGHANPDVVALSADVSNSDFSFMFQEA
                     FPERFFNVGIAEQALVDVAAGLAYSGKIPFANTFAIFLATRALEMVRTHLCYGEANVK
                     LMGAYAGLSDSFDGPTHHSITDIAIMRSLPNMTVIVPSDPVAVAKLLPQVAAWQGPVY
                     FRLNRNDVPVLFDESYAPRIGQAITLRPGNDVTLIATGLMVSRSLEAAQVLASEGIDA
                     RVLEVHTIKPLDVDRILQAARETGALVTAEEHSIIGGLGGAVAEVASEAYPVPIKRVG
                     LNDRFAETGPYQDLLDHYGMSVQAVIAAARQAVAAKR"
     gene            9381..10205
                     /locus_tag="VFF70_03555"
     CDS             9381..10205
                     /locus_tag="VFF70_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZY43802.1"
                     /translation="MKLAVVTSTPEVQLQIPVALLTGTFAERMQKAAQLGYDGVELMI
                     VQPAELNAEEIRAAVLQHGLQVAAVASGGIGFVDKLTLLNADEEVSRRAVERLHALID
                     LAAQLNAPLVTIGGFRGRLAWGGPTARARLIRILWKAADQAAAQSVRLALEPLNRYES
                     DIVNTVEEGLTLIDVVGHSHLGLLLDTFHMNIEEPSFHDSLRRARQADRLWHVHLGDS
                     NRLPPGQGHIDFAGIVSTLREIGYAGFLSAELWPRPDPDTAAAETIKYMRRWLPHA"
     gene            10236..11081
                     /locus_tag="VFF70_03560"
     CDS             10236..11081
                     /locus_tag="VFF70_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZY43803.1"
                     /translation="MTTIALFGAAGKMGTRISNRLKAAAQYQSLYVEVGDVGIAKLRE
                     RGVTPTSPDEAARSADVAILAVPDTILGKVAHSLVPLLKTGAMVICLDPAAPHGNELP
                     ARADIAYFITHPCHPPVVSDENDPEVRKDFFGGIKARQNVVCALMQGPEEDYAKGEQI
                     IRVIFAPVMNTYRITVEQMAILEPAMSETTILTCMFVMKEAIDEAVRRGVPKDAAYAF
                     ALGHFNINIGILFGYVDAQFSDGAKLAVERAKSQIFQPDWKKVFEPENVMEQVKAITQ
                     GRQKA"
     gene            11172..13157
                     /locus_tag="VFF70_03565"
     CDS             11172..13157
                     /locus_tag="VFF70_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43804.1"
                     /translation="MMLPKNVLYYGKNEPLPERLALRAGPLSLVYEEGDLRYIQLGER
                     EVLRRVYAAVRDRNWGTILPKFSNVQLDIQSDSFRIAYDVENKQGDIDFVWHGSIMGD
                     TQGTIAFTLDGVARSTFMKNRIGFCVLHPAACAAAACVVEHVDGTIERSKLPVFLVPD
                     QPVLPFAEMRAMTHEVLPGVWAEVRFSGDIFEMEDQRNWTDASYKTLCTPLRLPYPVE
                     IKAGTKVTQSITLRLKDERPVTGDRLSVSVGTSPLTFSLQPDVPAVPLPQIGLGVASH
                     DQPLSERELTRLQALQLHHLRVDLPLSDPACEATLRRASTEASALGIPLEAALFVSKN
                     GEAELRQFLPIIEQVRPTVGLWMIYPTKEIYSGGTPFAEVVTLARKYLENYDLKARWA
                     TGTNTDLIWLLRTPPPLELIDAVTFAITPQVHAFDNASVVETLEAQAAAALSARQLAG
                     TRPVIVSPVTFKMRHNPYATGAVPPTPAGQLPTQVDVRQMSLLGAGWTVGSLKYLAES
                     GVSRITYYETTGWRGVMELEGGSPLPNIFRSLPGSVFPLYHVLTDVGEFAGGEVIASR
                     SSDTQRVDGLSVRKADRVCAIVANMCDEPCQVAVQHLGNQVRVRYLDETNVIEAMQAP
                     ENFRAQSGEWLPTISGVGEIELLPYAVARIDTVIHSR"
     gene            13330..14871
                     /locus_tag="VFF70_03570"
     CDS             13330..14871
                     /locus_tag="VFF70_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZY43805.1"
                     /translation="MRSRKLFTVLVLIMVGTMILNACGAPATPAPPVVQTVVVKQTVI
                     ALQTVVAPTAVPANMTSAERAVSEARKYAGTTLNVTWEAALQSQDPLLFSGPKWEQLT
                     GIKINVVQLQFPDLYSKAVVEHIAGTGGYDVLNYSPAWTSDLAASGVLEPLDDYIDKY
                     MDKSEMADLAPFYAPAQTVAGKTYGLFDDGDIFVMYYRKDWFDDAKNQADFKAKFNRD
                     LTPPKTWQEWDEVCGFFTDKFKSQGMYGCALQRAEGQVFNWFYDHFRVNGGKFFDDNM
                     KATINSDAGVNTLTEMVNSNKFMPPGVEKWDFVAVLSAWLEGKVGMIITWPPIGRWSE
                     GYGTQTAQLSWVPKTQVAGKVGYAIPPGGHSEIAGAFNLGVSADSKNKEAAYLFIQWL
                     QSREISLDRVKLPFALRDPYRLSHYSDPLYQTLWPTAKDYLDTLKAAGEAGLADLALP
                     AAREYEDALDKAVTTVMGGGDPKAALDKAAADWDAITDRIGVDKQKAAYQDWVKVRGV
                     NAYPQ"
     gene            15098..>15936
                     /locus_tag="VFF70_03575"
     CDS             15098..>15936
                     /locus_tag="VFF70_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZY43806.1"
                     /translation="MPVLLVLPAVILLLGLGLFPLLYSLGISFTKWNLQDPAREFIGL
                     KNYIDVLGDQRMLGALRNTAIIMVFGVTFETLLGLGLAQTLVGHLPGKRFITPLLILP
                     AVVSPIVVGFTWRILYDATYGPIDHFLSLIRNQPTTIVWLANLQTVYPAVLLTEIWQW
                     TPFMFLVMLAGLTAVSPEIHEAASVDGASPWQIFTRITLPVIRPVVIVAVLFRTLDIF
                     KLFDIIVPLTGGSPGTTTETASLYLYILGFRNFRLGYSAAASYIILILFSVLLTLLLR
                     RIN"
BASE COUNT         3398 a         4310 c         4818 g         3410 t
ORIGIN      
        1 ggcggctacg acctgaacgt cgagtcgctg gacgcgatta agtccggcgc agcgcaggtg
       61 accgtcgggc agaatcctta tctgcagggc tatctgccgg tgctggcgct ggtggcgcag
      121 ttgcgcgacg gcaagccgcc catcaagggt tggctgaatg tgggcacgga agtggtgacc
      181 aaggacaacg tggacacgtt gtacaagcgt gagtccgatc cagcctacat gacgcagtgg
      241 tacgcagatt ggattgccaa gaacttcacc gatctgaacg cactgcaaaa accactgccg
      301 cagcattaag ctcggcagca tgatcgcagt gagccgcacg ggttcgcggg acttcagtcg
      361 tttgtaacca tgaacccgtg cgtgctccga aaatcaaaac aaatttcgac gttcaggagt
      421 tggcatggct gctcaacaag agatcattct tctggagaat atcaccaagc gttttggcgg
      481 ggtgacggct ctcgacgatg tgagctttgg catcgcgccg ggcgaagtgc acgccgtcgt
      541 cggcgaaaat ggcgccggca aatccacgct gatgaaaatt ttggcgggtg tgcatccgca
      601 ggataacgga aaagttattc tgcagggaac gccggtcgtg attcgggatc cgctcgacgc
      661 gcgcagtaaa ggcatcagca tcgtttttca ggaactgaat cttttccccg atctgagcgc
      721 cagtagcaac atcttcgtca accgcgaact ttcaggccgc gcgggccggc tcgatcagcg
      781 cgcgatggat gcagccaccc ggcaggtctt taccgcgatg gaattggaca tcgatccgca
      841 cgtcaaagtt aaaaaactct ccgtgggcga aaggcagctg gttgaaattg cccgcacttt
      901 gcaagcgcga tcagagatta tcatcatgga cgagccgaac tcggcgctga cgcagcgcga
      961 aaccgatcga ttattcgaac tcattcgcca tttgcgcgat caaggcatta ctatcatcta
     1021 tgtttcgcac cgtctggaag aagtctttgc cattgccgat cggatcactg tgctgcgcga
     1081 cggacactat caaggcacgt ggcgcacaac ggacacgaca atcccgaaaa ttgtggaagc
     1141 catgatcggc cggcggctgg aagaggcctt tcccgatcgc ccgctggtcc cggcggcggc
     1201 cgaggtgctg ctcgaagttc gcgatctaac ctgcggcaaa actggaccca ttagttttca
     1261 ggttcacgcc ggtgagatcc tcggcttcgc cgggctggaa ggcgctggcg tggaagatat
     1321 tttccgggtc ttgtttggcc tggatcgggt aaagagtggt gaagtgatct atgccggtca
     1381 agcgcgccgc gtacgatcgc cggtggaagc gctcaagttg ggctggggtc tcattccggc
     1441 gaaccgccgt gaacagggtc tgatgatcaa ttggccgatt cgcaagaata ccacgctggt
     1501 cattctcgat aaactgctca atcgcctggg gctgatcgac gcggccgccg accggcgcac
     1561 gacccaggaa tatgtggagc gcttgaatat caccacggac agcatagata agaaagtgat
     1621 caatctgagc ggtggcaatc agcagaaagt ggttgtcgcc aaatggttgg cgagcgggcc
     1681 caagatcttg atcctcgacg atccgacgcg cggcgtcgac gtgggcgcga agtcagagat
     1741 ttaccggctg tgcgaccaac tggcgcagca aggactggcc ttgttattca cctcgtctga
     1801 agtcgaagag attttgggac tgtgcgatcg cgtgctggcc ttctacaaag gtcagatgct
     1861 gcgcgaattc aagcgcggcg aggcgacgaa atcagacctg atgaacgtga tcgccagcgg
     1921 tgcaaatggg aatgcggtcc gaacgagtta gttttttcag cagcggcgtg ccgctcaccg
     1981 ggattttgac cttgccggac gatccggttg gccagcagcc ggcggtggtg ctcgctcacg
     2041 gctatgctaa ttacaaaggc gaattcggcg gctttgatga attggctgat gtcctggggc
     2101 gcagcggcta cgtcgctctt caatttgatt ttcgcggttg cggtgaaaca gacgcgcctg
     2161 tgggcaagat gttgtgtgcc accgaatggc cgctcgatgt gatgagcgca gtctcatatt
     2221 tgcaagcccg atcggaggtg gaccctcaac ggattggctt ggtcggccag agtatgggcg
     2281 gcggtgtggt cgccacggtg agcgcgctgg acgatcgggt ggcctgcact gtttctttgg
     2341 cgagtgtcag cgatggcgca cgctggctgc acgaaacgtg gacgcagcgg cgtggtgctt
     2401 cagggtggaa tgagtttttg atcgcgctgc aaaatgatcg gcatgaacgc gcgctgcatg
     2461 gacgttcgcg ctttgcgcca ctgcccgaac tcctggcatt gactgccgaa gagacggcaa
     2521 cttggctgga gatgcgggcg cgttatccgc tgtttttgta cgaagcgccg tgggaatcga
     2581 ttgacgatgt gctgcacttc aagccgattg atgtggtcca acatattcag tgtccgatcc
     2641 gttttgttca cggcacggct gatttgatgg tggacagcag tcacagcgtc gacatgcacg
     2701 cgcacgcggg ttcgcaggcc gatttgcaac tgatcgacgg tgcggaccac gctttgccga
     2761 tcgggacgca caaaggccgt gtgcaagaac tgatcgctga atggttggat cgttatctta
     2821 aatagcctgc atacgtttcg tagtttgcgc tgcgcaggag gatcatgaga tgaaggctct
     2881 agtcaaagaa cgtcctgaac ccggtttgtc gctgttgaac atcgccgagc ccaagatcgc
     2941 caacccggac gacgtgctgt tcaaagttga gtattgcgcc atctgtgtgg gcgaaaccaa
     3001 ggcttacgat tggaatgaat gggcggccaa cgacaagacc tttaagctgc ccacggttct
     3061 gggccatgaa gcagtgggtg tcgtcgtcga agtgggcagc gaggtcaaac aattcaagct
     3121 cggcgatcgc atcgtcaacg atccgctcat ctattgcggc tattgccgcc agtgccgcag
     3181 cggcttcacg aacatgtgtg aaaaccgcga gatttacggc aagcggcgcg gcgcattcgc
     3241 cgaatgtgcc gtactgccgg aacgcgtgct cgctcatctg cccgacaaat tgagcctggc
     3301 ggaaggcgcg gtgctggaga acttcggtat tgcgatgcat gccgtggata tcgaggaaca
     3361 cgaaccgggc gagtgggccg tcgttctcgg cgcgggaccg atcgggattt tggccgcgca
     3421 gatcctcacg gcctggggcg cgaatgtcgt gatcaccgat ctatcggacg cgcggctgga
     3481 ctttgcgcgt caacacaccc gcgccatcgc gattgacatc aaacaagaag accccgtcaa
     3541 gaaagtgctg gaattgacca agggccaagg cgctgatttt gtgatcgaga tggcggctaa
     3601 tcaagcggcg atggatcagg cttttgatat ggtgcgcatt cgcggcacgg tcgtcacgat
     3661 gggctcgttc aacggcccga tcacgttcaa tccgttcttc aagatgacgc ggcgtgagat
     3721 taaactgcag agcatgatgg gccgcacctg ggaatcgtgg cgccgcatgg tacagatgat
     3781 cgagaaggat caaatcgatc tgaagccgtt gatcacccac atcctgccgt ttgaagaata
     3841 tcaacgcggg tttgagctgg ttaaaggcca cgaggtgatg aagatcttgc tgaagccgta
     3901 atgagagatg cgttgtaagt agagcagccc catgccctgc ctaactggcc cgcgaaacgg
     3961 gatgggatgc gatttctgca gcctggttga atagcgtgag ttgtgaatta tttgaatgaa
     4021 cacggagacc tgtgagatgg caaacttcga tcaagcacgc accttactac gacaattcaa
     4081 aggcgacact tacttgcacg gcctgcacgt cttgcctcaa gttggatcgg tgacagccca
     4141 agagggcaag cgggcagtgt tggtgcgaaa tactttcacc ggatcagaag tctttacgca
     4201 gacgctcaaa gcatccctcg cggcggctgg cgttcaactg ctgggcgagg tggagggcgc
     4261 tgcacccaat gccccgcgcg aagatctgtt ccgcatcacc acagctttga ttgatctgca
     4321 gcccgacgtc ctggtcagct tcggcggcgg cagcaccatc gacgcgacga aggcggcgga
     4381 agtgcttcgc acgctgggcg gcagcatcga cgactacttt ggcactggac tggtgacgca
     4441 agcactcgct cgcggtggaa aaaaactttc accgcatgtg gcgattcaaa ctgcttccag
     4501 ttctgcggcg catttgacca agtattccaa catcaccgat gtgtccaccg ggcaaaagaa
     4561 gctcatcgtc gataacgcga tcgtgccgcc gcgtcctgtc ttcgattaca gcgtcacgtt
     4621 tgatgcgccg atctcattga cctcggacgg tgcgctggac ggcatctcgc actcgctcga
     4681 agtgctgtac tcagcggtcg gcaagccgga gtatgccacc atcgaagccg tcgccgccga
     4741 atgtattcgc ctgatcgtga attatctgcc cgcggtgttg aacaatccgc gcgaacccgt
     4801 tgggcgtgag gcgctaggct tggccaccga tctcggcggt tactcgatca tgctgggcgg
     4861 caccaacggc gcgcacctca ccagcttttc gctggtcgat ctgatgagtc acgggcgagc
     4921 gtgcggcctg ttgaatccct attacaccgt gttctttgcg ccggccgtac aagcgccgct
     4981 caaagtcgtc ggacagattt atcgggatgc ggggtacacg caagccgaca tcgatcagtt
     5041 aagcgggcgc gatctgggcg tcgccgtggc cgaagccatg atcgccttcg agcagaagat
     5101 cgggctgccg acaaccttga acgaagtgcc aggcttcagc gaggcttaca tcgatcgcgc
     5161 attgaccgcc gccaggaatc cgcagctcaa gatgaagttg gagaatatgc cggtgaagct
     5221 caacgccgag acgatcgatg aatatatggg gtcggtttta caggcggcca agaccggcga
     5281 tttgaagttg atcaagaacg taacatagtt ttcttttgac catcggccag gggatggcgt
     5341 tttgcaacca cactgttcga tcaggagatc agcatgaaac ttggagtgaa cacttacacg
     5401 tacatgtggt cgatcgggtt taaggggcca aacccggctt tccccgataa cgaggcccgt
     5461 cctgaaagac ccctcaccgc gttgggcctg ctggaaaaag cgcgcgcgct tggtctgcat
     5521 ctggtacaga ccggcccgaa cttgccggtc gataaattgc ccgaagcaga gtttgaggcg
     5581 ttcattcagc aagcgcgggc gtggaacatt gaattggaac tgggcacgcg cggcctggac
     5641 tacgatcatc tggtgaagca ggtcgccatg gccaaacgca tgggcgcgaa gttgatccgc
     5701 acgctgccgg aaattgaagg gaaatatgcc accgatgcgc gcttgatccc tccggtggtg
     5761 cgccagattg tcccggtgct ggaacgtgag ggcgtccgct tagcgatcga gaacggcaag
     5821 acccctgcgg tcaatctgcg cgcggcgctc gacgagattc atagtccgtg cgtcggcctt
     5881 gtgctggata tggtgaactc gctggcaata cccgaaggct ggagatatgt gacgcagctg
     5941 ctggctcccc atacgatgtg cgtgcactac aaggacttta ccatcaaacg tgcctggcac
     6001 atgatgggct ttatttgtga aggcatgccg gcgggtaagg gcttggttga gaccgagtgg
     6061 ctgctgaacg aactcaaagc ctcgccttac gatttcaacg tcatcatcga gttgtggccg
     6121 ccggagcagg agaaattgca ggacacgatc gatctggaac aggcgtgggc gatcgagagt
     6181 gtgccgtatt tgcggaaatt catcaaagat tgacgaacgc agccagccag aggcgggact
     6241 tctatgcaaa ttcgattggc ttatggaaaa acggggttga tggttgaact gcccgacgat
     6301 cgatcggtga ccgtcgtcga gccgcagttc gtgccgggtc tgccggacgc gcgcgaagcc
     6361 ttgcgacagg ctctgcgcac gccgttgaat tcaacgccgc tgcgtgagct ggcaaagccg
     6421 acggacaaaa tcggcattgt cttcagtgac atcacgcgcc ccacaccgca ccacttgttg
     6481 ataccgtccg tccttgaaga gctggcgcac gttccccgcg aaaacatcac gctgttcaat
     6541 gcgctgggga cgcatcgccc gaataccgag gccgaactgc gcggcatgtt gggcgatgcg
     6601 ctggtggatg gctatcgcct tgtgcagaac aactcctttg atccggcgac gcaggtgcgc
     6661 gtgggtgtga cctcgcgcgg gcacgagatc tggctcaacc gtgaagttgc tggatgtgat
     6721 ctgaaggtcc tgacgggttt catcgagccg catttcttcg cgggcttttc gggcggcggc
     6781 aaagcgttga tgcccggcat ggccggtcaa cgcacggtgc tgggcaatca cgatgcaggc
     6841 atgatcggca atccccaggc cacgtggggt atcacctggg gcaatccgat ctgggaagaa
     6901 gtgcgcgagg ttgcgctgca aaccgagcgg ttattcctat tcaatgtcac acttaacaaa
     6961 gataaggaaa ttaccggcgt ttttgcgggc gacttggatg cggcgcatgc agcaggctgc
     7021 gcatttgtga aacagactgc gatggtcgcc gtgccgcggc cgttcgacat cgtcatcacg
     7081 accaactccg gctatccgct tgacctgaat ctctatcaag ccgttaaggg catgagtgcg
     7141 gcggcgcagg ttgtgcgacc gggcggagcc atcatcgtcg cggccgattg ttgggatggc
     7201 attcccgatc acggtctgta cggtcaattg ttgcgcgagg ccagcagccc gcaagctctg
     7261 ctcgatcgaa tctgtgcgcc gggcttcttg gaacaagatc aatggcaagc gcagattcaa
     7321 gcgatcattc agctcaaggc cgatgtctac gtgcgcaccg acaacttgac tgacgcgcag
     7381 atcgaagcag cgttgctgaa gccatgtcat tgcatcgaag acacggtggc cgaattgttg
     7441 gatcgttatg gcctggaagc gaccatctgt gtgttacccg aggggtcgca gacgatccct
     7501 tacatcgcgt agcggcaaaa gaatttttac cgtagaggtt tgagcccatg cactatcatt
     7561 cagaagaact ggtgaaagac ctttcggtca aagcacaacg ttttcgcatt cacgtgttga
     7621 agatggttta ccatgcgcag accggccacc tcggcggcgc gttttcggta gccgaaattg
     7681 tcgcttcact ctactttcac catttgcaag tcgatcctgc tcagcccgat tggattgatc
     7741 gcgatcgttt gttgttctct aaaggccatg cctgcgcgat gttgtacacc gcgttggcgt
     7801 atcgcggctt tttcccagtc gaggaattgg cgacttttcg ccaattcaat agtcgcctgc
     7861 agggccatcc cgatcggttg aagacgcccg gcgtggaaat ctgcgcgggg ccgttggggc
     7921 acggtatcgc gatcggtgcg ggcatggccc tcgcgcagaa gatgcgcggc ggaaaacctt
     7981 catcactcag tgcgccctcg gccagagctt cggccggtcg ggtgtacgtc gtgctgggcg
     8041 acggcgagct cgacgcgggc gtgatctggg agggcgccat gacggcggcc aaatatcgtc
     8101 taggcaactt gacggcgatc ctggattaca acggtattca acaaaccggc gcgacggccg
     8161 atgtgatgcc gaccgaaccg atcgcggcga aatgggaagc gtttggctgg catgtgcaag
     8221 aagttcacgg ccacaacgtc gccgagattc tggccgcgct cgatcgggcg gatgaagtgc
     8281 acgcgcggcc cagcgtaatc atcgcgcgta cgaccaaagg caaaggcgtc agtttcatgg
     8341 aatacgacca tcgctggcac gggatgccgc ctaaccgaga acaatacgaa aaggcgctgg
     8401 ccgaattaga agcgggagtg caagcatggc agaactagcc ccgatgcgta aagcctatgg
     8461 cgaagcactg gtcaaactgg gccacgccaa tcctgacgtg gtggcgctca gcgctgatgt
     8521 gtctaattcc gattttagtt tcatgtttca ggaagctttt ccagagcgct tttttaacgt
     8581 cggtattgcc gagcaggcgc tggtcgatgt ggccgccggc ctggcctata gcggcaagat
     8641 tccatttgcc aacacgtttg caattttctt ggcgacgcgc gcgctggaaa tggtgcgcac
     8701 gcacctgtgt tacggcgagg ccaacgtcaa attgatgggc gcgtatgcgg gcctttcgga
     8761 ttctttcgac ggtccgactc atcattccat caccgatatc gccatcatgc gcagcctgcc
     8821 taacatgacg gtgatcgtgc cgagcgatcc cgtcgccgtc gcaaagctgc tgccgcaagt
     8881 cgccgcatgg caaggcccag tctacttccg cctaaatcgc aatgatgtgc cggtgctgtt
     8941 tgacgagagt tacgccccgc gtatcggaca ggcgatcacg ctgcgaccgg gtaatgatgt
     9001 gacgctgatt gcgaccggcc tgatggtgag tcgatctttg gaagccgccc aagtgttggc
     9061 cagtgaaggc atcgacgcgc gcgtgctgga agtgcacacg atcaagccgc tcgatgtaga
     9121 ccgcattctg caagcagcgc gtgaaaccgg cgctttagtg accgcggaag aacacagtat
     9181 catcggcggg ttgggaggcg cggtggccga agttgcgagc gaggcttatc ctgtgccgat
     9241 caagcgcgtg ggcctcaacg atcgctttgc cgagaccggc ccgtatcaag acctgctgga
     9301 tcactatggc atgagtgtgc aggctgtgat cgctgcggca cgacaggccg ttgcggcgaa
     9361 gcgatgatga gaggcgcgtc gtgaagctgg ccgtcgtcac ttctacaccg gaagttcaac
     9421 ttcagattcc ggtcgctttg ctcacaggca cttttgccga gcgaatgcaa aaggccgccc
     9481 agctgggtta tgacggcgtc gagttgatga tcgtccaacc cgctgaattg aatgcggaag
     9541 agattcgagc agcagtcctg cagcatggct tgcaggttgc cgcagtcgcc agcggcggta
     9601 tcggctttgt cgataagttg acgctgttga atgcggatga ggaggtcagc cggcgagccg
     9661 ttgaacgatt acatgccttg atcgatctgg cggcgcagtt gaacgcaccg ctggtgacca
     9721 tcggcggatt ccgaggccga ttggcgtggg gcggcccgac agcccgcgcc cggctgatca
     9781 ggatactgtg gaaagccgcc gatcaggcag cggcgcaaag cgtgcgtctg gcgctggaac
     9841 cgctcaatcg ttatgaatcc gacatcgtca acacggtgga agaaggacta acgctgatcg
     9901 acgtagtagg tcacagccat ctgggcttgc tactcgatac gtttcacatg aacatcgagg
     9961 agccatcatt tcacgattcg ttgcggcggg cgcggcaagc cgatcggttg tggcacgtcc
    10021 atctgggcga cagcaatcgc ctaccgccgg ggcaggggca tatcgacttt gccggcatcg
    10081 tgtccacttt gcgcgagatt ggctacgcgg gatttttgtc ggcagaactg tggccgcgtc
    10141 ccgatcccga tacggctgcg gcagaaacga tcaagtacat gcggcgatgg cttccgcatg
    10201 catagatact aaatctcaat aaggagcgaa acaacatgac tacaattgca ttgtttggcg
    10261 cagcgggtaa gatgggcacg cgcatttcca atcgattaaa ggctgccgcg caatatcaaa
    10321 gcctctacgt cgaggtgggc gatgtcggca tcgctaaatt gcgcgagcgc ggcgtgacgc
    10381 cgacttcacc cgacgaggcc gcgcgcagcg ccgatgtggc gatcctcgcc gtgcccgata
    10441 cgattctagg aaaggtggcg cacagtctcg tgccgctgct caagaccggc gcgatggtga
    10501 tctgcctcga tccggcggcg ccgcacggca acgaactgcc cgcgcgagcg gacatcgcct
    10561 acttcatcac gcacccttgt cacccgcctg tcgttagcga cgaaaatgat cctgaagtgc
    10621 gcaaagactt tttcggcggc atcaaggcaa ggcagaacgt agtctgcgct ttaatgcaag
    10681 gtccggaaga ggactatgcc aaaggcgagc aaatcattcg ggttatcttc gcgccggtga
    10741 tgaacaccta tcgcattacg gtggagcaga tggcgatcct cgaaccggcg atgtcagaga
    10801 cgacgattct gacttgcatg ttcgtgatga aagaagcgat tgatgaagcg gtgcgccggg
    10861 gtgtgccaaa agatgcagcc tatgcctttg cgctcggcca cttcaacatc aacattggca
    10921 tcctgttcgg ctatgtcgat gcgcagttct ctgacggcgc aaagttggcc gtcgagcgtg
    10981 ccaagtcgca gattttccag ccggattgga agaaggtctt cgagccggag aacgtcatgg
    11041 agcaggtcaa ggccattacg caaggccggc agaaggcgta gattgatcca cgccgggcac
    11101 gcagaacctc taagttttgc tacgtgtctg gtgtggcttg cttttcatgg attgtttata
    11161 gcaggcgagc gatgatgtta cccaagaacg ttctgtacta cggcaaaaac gaaccgctgc
    11221 cggagcgtct cgccttgcgc gcggggccgc tgtcgctggt ttacgaagaa ggcgatctgc
    11281 gctacatcca gctgggcgaa cgcgaagtgc tgcgccgcgt gtatgccgcc gtccgcgatc
    11341 gaaattgggg cacgatcctg ccgaaattct ctaacgtgca gctggatatt caaagcgatt
    11401 catttcgcat cgcctatgat gtggagaaca agcagggtga catcgacttc gtctggcacg
    11461 gatcgatcat gggcgatacg cagggcacga tcgcgttcac gctggatggc gtggcgcgtt
    11521 caacgttcat gaaaaaccgc atcggttttt gcgtgctgca tccggctgcc tgtgccgctg
    11581 cggcctgcgt agtggagcat gtggatggca cgatcgaacg atcgaagttg ccggtcttcc
    11641 tcgtgcccga tcaaccggtg ctgcctttcg cggaaatgcg agcgatgacg cacgaagtgc
    11701 tgcccggcgt gtgggccgaa gtgcgtttca gcggcgacat cttcgagatg gaagatcaac
    11761 gcaactggac cgatgcttct tacaagaccc tctgcacacc gctgcgtttg ccgtatccgg
    11821 tcgagatcaa agcgggcacg aaagttacgc agtcgatcac gttgagactc aaagacgagc
    11881 gacccgtgac tggcgatcga ctttcagtct ccgttggcac atcaccgctt accttttcgt
    11941 tgcaacctga tgtgcctgcc gttcctctgc cacagatcgg cttaggggtc gccagtcacg
    12001 atcaaccgtt gagcgagcgc gaactgacgc gcttgcaggc tttgcagctg catcacttgc
    12061 gcgtcgatct gcctctctcc gatccggcct gcgaagccac actgcgtcgc gcctcgacgg
    12121 aagccagtgc gctgggcatt ccgttggaag cagcgctctt cgtttcgaaa aatggcgaag
    12181 ctgaattgcg ccaatttctg ccgattatcg aacaagtccg gccaaccgtt ggcctgtgga
    12241 tgatctatcc aaccaaggaa atctatagcg gtggcacgcc cttcgctgag gtcgtcacgc
    12301 tcgcgcgcaa gtacctggaa aattacgacc tcaaggcgcg ctgggcgacg ggcacgaata
    12361 ccgatctgat ctggctcttg cgcacgcctc cgcctctcga actgatcgat gcagtgacgt
    12421 ttgcgatcac gccgcaggtt catgcgtttg acaatgcctc ggtggtggaa acgcttgaag
    12481 cgcaagccgc ggccgcgctg agcgcccggc aattagcggg cactcggcct gtgatcgtaa
    12541 gtccggtgac gtttaagatg cgtcacaacc cctacgccac tggcgctgtg ccgccaaccc
    12601 cggcggggca actgcctacc caagtcgatg tgcggcagat gtcgctgctg ggcgcgggtt
    12661 ggacggttgg cagcctgaag tatctggctg aaagtggcgt gagccgcatc acttactatg
    12721 aaacgaccgg ctggcgcggc gtgatggagc ttgagggtgg atcacctctg ccgaatattt
    12781 tccgatcact gcccggttcc gtcttcccgc tgtatcacgt gctgaccgat gtgggcgaat
    12841 ttgccggcgg cgaagtgatc gccagtcgat cgagcgacac gcaacgcgtt gatggcctca
    12901 gcgtgcgtaa agccgatcgg gtgtgcgcca tcgtcgcgaa tatgtgtgac gagccgtgcc
    12961 aggtcgcagt gcaacatctg gggaaccagg tgcgcgtgcg ctatctggac gaaaccaatg
    13021 tgattgaagc gatgcaagcc ccagagaatt tccgtgctca atcgggtgaa tggctgccga
    13081 ccatctcagg cgtcggtgaa atcgaattgc ttccatacgc agtcgcccga atcgataccg
    13141 tgattcattc acgttagcgt gcggaagtga tcgatggctt gtcgaggtcg acattcaagc
    13201 cgcgctcttg agcgaagcga gcgcaaactt gaacagagta gtaaaggagg tggatctgca
    13261 ctgaaaatcc ggcttttctt gtcaggtaat tagatccaat caaattcaca gaaaatcgag
    13321 gaggtaacaa tgcgttcacg gaagttgttc acagtgttag tgttgatcat ggtgggaacc
    13381 atgatcttaa atgcatgcgg tgcgcctgca acaccggctc cacctgttgt gcagactgtg
    13441 gtggtgaagc aaacggtcat cgccctacag accgtggtag cgccgacggc tgttccggct
    13501 aatatgacgt cggccgagcg cgccgtgagc gaggcccgca agtacgccgg cacgacgctt
    13561 aacgtcacct gggaagccgc cttgcaatcg caagacccgc tcctcttctc agggcctaag
    13621 tgggagcagt tgacgggaat caagatcaac gtcgtgcagc tgcaattccc ggatctgtat
    13681 tccaaggccg tggtggagca tatcgcagga acaggcgggt atgacgtctt gaactattcg
    13741 ccggcttgga catccgacct ggctgcgtct ggtgttcttg agccactcga cgattacatc
    13801 gacaagtaca tggacaagtc cgagatggcc gacctggcgc cgttttacgc tccggctcaa
    13861 acggtggccg gcaagactta cggcctcttt gacgacggag acatcttcgt gatgtactat
    13921 cgcaaagact ggttcgatga cgctaagaac caggccgact tcaaagccaa gttcaatcgg
    13981 gatctgacgc cgcccaagac gtggcaagag tgggatgaag tctgcggctt cttcaccgac
    14041 aagttcaaaa gccagggcat gtacggctgt gcactgcaac gcgcagaagg acaggtcttt
    14101 aattggtttt acgatcactt ccgcgtaaac ggtggcaagt tttttgacga caacatgaaa
    14161 gcgaccatca acagtgatgc tggcgtcaac actctgaccg agatggtgaa ttcgaataag
    14221 ttcatgccgc ccggtgttga gaagtgggac ttcgttgccg tgttgagcgc gtggttggaa
    14281 ggcaaggtcg gcatgatcat cacctggccg cccatcggcc gctggtctga aggctacggc
    14341 acgcagaccg ctcagttatc ctgggtgccc aagactcagg tcgctggaaa ggtaggttat
    14401 gccattccgc ccggtggtca tagtgagata gcgggcgctt ttaacctcgg cgtttcggcg
    14461 gacagcaaga acaaagaagc tgcttatctc ttcatccagt ggttacagag tcgtgagatc
    14521 agcctagatc gtgtaaagct gcccttcgcg ctgcgcgatc cgtatcgcct atcgcactat
    14581 tctgatccgc tgtatcaaac cttgtggccg acagcgaaag attatctgga cacgctgaaa
    14641 gccgccggcg aagcgggctt ggccgatctg gcattgcctg cggcgcgtga gtatgaagat
    14701 gcgctggata aagcggtaac caccgtcatg ggtggtggcg atcccaaagc ggcgttggat
    14761 aaggccgcag ccgattggga tgcgatcacc gatcggattg gtgtggacaa gcagaaggcg
    14821 gcgtatcagg actgggtcaa agtccgaggc gtaaacgctt atccccaata aatttcgctc
    14881 attcacgtga cgagcgggta tgacagatcg ttcgacgact cgcttgacac ttgtttagcg
    14941 actcaagaat ctgcattcct ccagacgttg agtcgggcgc ttggataagg acccgactca
    15001 acctcaaggg gctgcgatga ctaagatgac tgtgacgcca acagcaccgc caacgacaac
    15061 ccaatcggct cgactcagtg cctggttcga gcgacgtatg ccggtattac tcgttctacc
    15121 cgctgtgatt cttctattgg gactggggct ctttcctctg ttgtattccc ttggcattag
    15181 ttttacgaaa tggaatttac aggacccggc gcgggaattc attgggctaa aaaactacat
    15241 cgacgtgctg ggcgaccagc ggatgttagg agcactgcgc aacaccgcca tcatcatggt
    15301 ctttggtgtc acgttcgaaa cgttgttagg actggggttg gcgcaaacac tggtcggcca
    15361 tttgcccggc aaacgtttta tcacgccgct gttgattcta ccggcagtcg tgtcaccgat
    15421 cgtggtaggt tttacctggc ggattcttta tgatgccact tatggaccta ttgaccattt
    15481 cctaagttta atccgcaacc aaccgacaac gattgtctgg ctggctaatt tacaaacggt
    15541 ctatccggct gttctcttga ccgagatctg gcaatggacg ccgtttatgt tcctggttat
    15601 gttggctgga ctcacggccg tcagtcctga aatacacgaa gcggcctccg tagacggcgc
    15661 atcgccgtgg caaatcttta cgcggatcac gctgcccgtt attcgaccgg tcgtcatcgt
    15721 cgcggtcctc ttccgcacgc tcgatatttt taagctattc gacatcatcg tgccgctgac
    15781 gggcggcagt cctggcacaa ccaccgagac agcttcgcta tacctgtaca tcttgggttt
    15841 caggaatttt cgtttagggt attccgccgc tgcgtcttac atcatcctca tcctattctc
    15901 ggtgctgctg acactgctgt tgcgacgcat taacaa
//
LOCUS       DASRTJ010000167         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_84887,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000167 DASRTJ010000000
VERSION     DASRTJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84887"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFF70_03580"
     CDS             complement(<1..1356)
                     /locus_tag="VFF70_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43807.1"
                     /translation="MNTTHPIDHTLQQRVASLRWILPIILIVLTAIYELGPGHLLQSK
                     PNDPVDIEILLYGVVSPLLAFWTLTLIGRWLEKTDRAEKQAQAIDRRLASILSASADA
                     ILVLDPEGRIDTWNRGAEELFGYSTQQIKGQPLSDLFGKTEAALIEVQWLKQTVQKAG
                     FVRGYETTCHEIDGRALAIELTATRLTDEQSQPIGMSVILRDVTERKRREEEIYRLNT
                     NLSEQVADRTRELAQKVEELARANVELQKLDQMRTEFVSLVSHQLRAPLTNMRGATER
                     MLDQCGAINATCSRMFSVLDQQVDRLDRLVQDVLSATRIEAGELTLQSEPISVLPIVQ
                     QIVDQTRSRHDARSIHLPTKPGLPLVFADRDRVAEVLANLLDNADKYSPHDREIDIDV
                     QADQTEVTITVRDFGKGLPPNDLDRIFDKFYRADNSDAQAAYGYGLGLYVCRRLIEAQ
                     GG"
     gene            complement(1510..2667)
                     /locus_tag="VFF70_03585"
     CDS             complement(1510..2667)
                     /locus_tag="VFF70_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HZY43808.1"
                     /translation="MPLVQIDRDYLIQTLTNLVMINSINPSLVADGAGEAHIAAYVAD
                     ALRQIGCEVKLHDPQPGRVSVVGILKGTGGGRSLMLNAHMDTVGVDNMPDPFSGAIRD
                     GKLYGRGAYDMKGSLAACIAAAKALSESDRLTGDVLIAAVADEEYASLGTEEIAKHYQ
                     PDAAIVTEPTELTLCIAHKGFVWIEVETIGRAAHGSRFALGIDANMRMGRFLAELDRL
                     EQALRNRIGHVLVGPPSLHAAKIEGGTEPSMYAARCALQIERRTIPGESTAQVLQEIQ
                     TIIDKLSKADPTFKASIKSSLARDSFEVSREAPIVRSIDRAATHVLGHPPEYTGVSFW
                     MDSALLAAAGIETAVFGPLGAGAHASEEWVDLQSVEDTARILQQTAIDYCG"
     gene            complement(2852..>3676)
                     /locus_tag="VFF70_03590"
     CDS             complement(2852..>3676)
                     /locus_tag="VFF70_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZY43809.1"
                     /translation="LLLNEGLLDSQAATGTGRMQIEARAQKARSIEAGALIFISNCST
                     CHGDNGEGVPGKGLALNPDLFTKHYPAIQAARTFNGTLHDFIKATVASGRPVPSAYAD
                     SQGGFANPMPTWSQQFGGPLRDDQIEDVTNFILNWEAQAMAGPVLPPFNGIGADLTVP
                     LPTGDAARGADLFAQKVKFESGSAAPCKACHSLQPNTVIVGPSLSDIGMMGATMVPGK
                     AAADYIRESVQNPSAFIVPGNEKFVNNGKSLMPEGLGNNMSAQDLADLIAYLLTLK"
BASE COUNT          832 a         1075 c          949 g          820 t
ORIGIN      
        1 gccgccctgc gcctcgatca agcggcggca aacatacaag cccagtccgt aaccatatgc
       61 ggcctgcgca tcgctgttgt ccgcgcgata gaacttgtcg aagatgcgat cgagatcatt
      121 cggcggcaaa cctttgccga aatcgcgcac cgtgatcgtc acctcggttt gatccgcctg
      181 cacgtcgata tcgatctccc gatcgtgcgg cgaatactta tccgcgttgt cgagcaagtt
      241 tgccaaaact tctgcgaccc gatcgcgatc ggcgaacacc aacggcaggc ccggcttggt
      301 cggcaggtgg atcgatcggg catcgtggcg cgatcgggtt tgatcgacaa tttgttgaac
      361 gatcggcagc accgagatcg gttcactttg caacgtcaat tcacccgctt cgatccgcgt
      421 cgcgctcaac acatcctgca ctaaccgatc gaggcgatcc acttgttgat cgagcacgct
      481 gaacatgcgc gagcaagtgg cattgatcgc gccgcactga tccaacatgc gctcggtcgc
      541 gccgcgcata ttcgtcaacg gcgcgcgtaa ttgatgcgag actagcgata caaactccgt
      601 tcgcatctga tcgagtttct gcaactcgac gttcgcccgc gccagttctt caactttctg
      661 cgccagttca cgcgtgcgat cggccacctg ttcgctcaag ttagtgttga ggcgataaat
      721 ttcttcttcg cggcgcttgc gctccgtcac atcccgcaga atcaccgaca tgccgatcgg
      781 ttgactctgc tcatcggtca accgcgtggc cgtcaattca atcgcaagcg cgcgtccatc
      841 tatctcgtga caagtcgttt cgtatccgcg aacaaagcct gccttttgaa cagtttgttt
      901 cagccattga acttcgatca aagccgcttc tgtcttgcca aacaaatccg acaacggttg
      961 acccttgatc tgttgggtcg agtaaccgaa caattcttcc gcgccacgat tccacgtatc
     1021 aatgcggcct tccggatcga gcaccaagat cgcatcggcg gaggccgaca agatcgaagc
     1081 gagtcgtcga tcgatcgctt gagcttgctt ctctgcccga tcggttttct ccagccaacg
     1141 gccgatcagc gtcaacgtcc aaaatgccag cagcggactg accacgccgt acaaaagaat
     1201 ttcaatatca accggatcgt tcggcttgct ttgcagcaaa tggcccggcc ccaactcata
     1261 gatcgccgtc aagacgatca aaatgatggg caatatccag cgtagactgg ccacgcgttg
     1321 ttgcagcgta tgatcgatcg gatgagttgt attcatatgc accgtcctaa cagagacaca
     1381 tcgacagatg ataaatgatt ctacatcaac ccggaggcga aagcgaattc aatcgtgcga
     1441 ggcaaattat caatacagat tgatcaataa gagacggacg cagttcgaga atatggaagg
     1501 caagtgatct caaccgcaat aatcgatggc ggtttgctgc aaaattcgcg ccgtgtcttc
     1561 cacggattgc agatcgaccc actcttcgct ggcatgtgcg cccgcgccca gtgggccaaa
     1621 gacggccgtt tctattccgg cggcggccaa taaggccgaa tccatccaga acgatacgcc
     1681 cgtgtattcc ggtggatggc ctaatacatg agtcgccgcg cgatcgatcg aacgaacgat
     1741 cggggcttcg cgcgaaactt caaaagaatc tcgcgccaat gatgatttga ttgacgcctt
     1801 gaaagtggga tcggctttgg aaagtttatc gatgatcgtt tggatttcct gcaagacttg
     1861 ggcggtcgat tcgcccggaa tggtgcgccg ctcgatctgc agggcacagc gcgccgcata
     1921 catactgggt tcggtgccgc cttcgatctt cgcggcgtgc aatgaaggcg gaccgaccaa
     1981 cacatgaccg atgcgattcc tgagggcttg ctccagccga tcgagttcag ctaagaaccg
     2041 gcccatgcgc atgttggcat cgatgcccaa agcaaatcgg ctgccgtgcg ccgcgcgtcc
     2101 gatcgtttca acttcgatcc acacgaaccc tttgtgcgcg atgcacaacg tcaactcggt
     2161 cggttcggtg acgattgccg catcaggctg gtagtgcttg gcgatttctt ccgtgccgag
     2221 gctggcatat tcttcgtcgg cgacagcggc gatcaacaca tcgcctgtca atcgatcgct
     2281 ttcacttaac gccttcgccg ctgcgataca cgcggccaga ctgcccttca tatcatatgc
     2341 gccgcgacca tataacttgc catcacgaat tgctcccgaa aatggatcgg gcatattgtc
     2401 aacaccgacg gtgtccatgt gcgcattcaa catcaacgat cggccgccgc ccgtcccttt
     2461 gagaattccg acgacgctga ctcggcctgg ctgcgggtcg tgcagtttca cttcgcaacc
     2521 gatttgccgc aacgcgtcgg cgacgtaagc cgcgatatgc gcttcgcctg cgccatcggc
     2581 caccaatgat ggattgattg aattgatcat taccaaatta gtcaacgttt ggatcaaata
     2641 atcacgatcg atttgaacaa gcggcatagc ggcaacctca atagatgtgt gtacaccact
     2701 gcaagtttac cacgctcgac ggacagcgct tcaaatgcaa acggccacac cacaagggtc
     2761 aggccgtttg tcgatctttt ggaaaaacct tgcgaaggta aaatttgaaa atatcgaatc
     2821 tttctagaaa ccttcgcaag gatcagggca attacttcag cgtgagcaag tacgcgatca
     2881 aatcggccaa atcctgcgcc gacatgttgt tgcccaaacc ttcaggcatg agcgacttgc
     2941 cgttgtttac aaatttttca ttgccgggca cgataaacgc actcggattt tgaacactct
     3001 cgcggatgta atccgccgca gctttacccg gaaccatcgt cgcgcccatc atgccgatat
     3061 ccgacagtga cggaccaacg attactgtat tgggttgcaa ggagtgacag gctttgcatg
     3121 gcgccgcact gccgctttca aatttcactt tttgcgcgaa caaatctgcg ccacgagcgg
     3181 catcgccggt gggcagcggc accgtcaaat cagcaccaat accattgaat ggcggcagaa
     3241 ccgggccggc catggcctgc gcttcccaat tcaaaataaa gttggtcacg tcttctattt
     3301 gatcgtcgcg cagagggccg ccaaactgct gactccacgt cggcatggga ttggcgaagc
     3361 cgccctgact gtctgcatac gccgaaggca ccgggcgacc agatgccacc gtggccttaa
     3421 tgaaatcgtg cagcgtgccg ttgaaagttc gagccgcttg aatagctgga tagtgcttgg
     3481 taaacaaatc gggattcagc gcgagccctt tgccgggaac gccttcgccg ttatcgccgt
     3541 ggcaggtgga acagttgctg ataaagatca acgcccccgc ttcgatcgag cgggctttct
     3601 gcgcccgcgc ttcgatctgc attcgccccg tgcctgtggc cgcctggctg tctaacagac
     3661 cttcgttcag cagcaa
//
LOCUS       DASRTJ010000168         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_236537,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000168 DASRTJ010000000
VERSION     DASRTJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236537"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFF70_03595"
     CDS             <1..769
                     /locus_tag="VFF70_03595"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZY43810.1"
                     /translation="KMSEVRIVFVGSGASNVACSRLIFTSGANPALCMMVDSKGILNK
                     NRHDIELRKAEFIDKWKLCQITNAEQREGNIGDALKGADVVVALSTPGPDVIKAEWIK
                     GMAKDAIVFACANPVPEIWPWDAKNAGAAVVATGRSDFPNQVNNSLGFPGIFRGTLDV
                     RAKTITDEMCLAAAKALADMAAEKGITADYILPNMDEFDVFAREAAAVATKAVEQGIA
                     RKTVTYEEEYQHASSIIKNARDMTQLLMEEGFIAESE"
     gene            824..1780
                     /locus_tag="VFF70_03600"
     CDS             824..1780
                     /locus_tag="VFF70_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY43811.1"
                     /translation="MYDLIIIGGGPAGLTATIYALRKRLNVLLISKDLGGKTNFRLQL
                     PEVEHHLVINGEEVVSRFVNEIRYLDFSRIMDKVEKIEKIDRGYRVFTRGGKEHERPL
                     GEYDTRSIIIASGTQGQFLNVPGEKDYLMRGLCFSAMSYAPLFIERDTVVVGDDDLAM
                     RAAMELAQICKSVTLIAPTHGKLDSPIGKRLQTWPNVLILEGYQAKQVKGDSYARSLV
                     IGKNGDTRELKADAIFVELGLKPNSEMVKGLVDLEADGRIRIDAQNRTSAPGIFAAGD
                     VTNAYAEQVLIAIGEGAKAALAVHEYLMEQPIEQKAMAELWR"
     gene            1908..2426
                     /locus_tag="VFF70_03605"
     CDS             1908..2426
                     /locus_tag="VFF70_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZY43812.1"
                     /translation="MSKIDTLVQDFLAQKKIAVVGVSDKRDTGCNLAYRKFKAAGYTV
                     YGVNPRITTFEGDPIYPDLKAIPDRLEAVFILTNPKVTDQIVQQCVELGIKHVWMHCM
                     MGTKPGLVAGMTSVSPDAVRICRENGIAVIPGTCPNQFLEADFFHGLMRKMWRVFGFL
                     KISDNGSIKAKA"
     gene            2453..3082
                     /locus_tag="VFF70_03610"
     CDS             2453..3082
                     /locus_tag="VFF70_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZY43813.1"
                     /translation="MKKILVTYATFAGSTVEVAQAIGDEITKSGWQVDVLPIGEVKDV
                     EAYAGVVLGAPMIVGWHRAALTFLKRHRSVLQHSPLAIFVMAMSLTQTDETCIDGVPV
                     FVDEKLPKPSEKAGHLSFRERHTSLANYVPPILKATRPGKPVSVGVFGSRLEYRRLKW
                     WAMLFVMLIVQAPARDRRNWTAIRSWAAGLAAALQLEELELQLTTGAQN"
BASE COUNT          791 a          888 c          967 g          682 t
ORIGIN      
        1 gaagatgtcc gaagtccgaa ttgtcttcgt cggcagcggc gcatcgaatg tggcttgctc
       61 ccgattgatc ttcacctccg gcgcgaatcc tgcgctgtgc atgatggtcg acagcaaagg
      121 cattctcaac aaaaaccggc acgatattga actgcgcaag gctgagttca tcgataaatg
      181 gaagctgtgc cagatcacca atgccgagca acgtgaaggc aacattggtg acgcactgaa
      241 aggcgcggat gtggtcgtgg cgctctccac ccccggaccg gatgtgatca aagctgaatg
      301 gatcaaaggc atggcaaaag atgcgatcgt gtttgcctgc gcgaatccgg ttccggagat
      361 ttggccatgg gatgcgaaga acgctggcgc cgcagtcgtc gccaccggcc gatcggattt
      421 cccgaaccaa gtcaataact cgctcggttt cccgggcatc ttccgcggca ccttggatgt
      481 tcgcgcgaag acgatcaccg atgagatgtg cctggctgcg gccaaggcct tggccgacat
      541 ggccgccgag aaaggcatca cagccgatta cattctgccg aacatggacg agttcgatgt
      601 gttcgcccgc gaggccgccg cggtggcgac gaaggccgtc gagcagggca tcgcccgcaa
      661 gacggtgact tacgaggaag aatatcaaca cgcctcgtcg atcatcaaga acgcgcgcga
      721 catgacacag ctcttgatgg aagaaggttt catcgctgaa tctgaatagc aatcgcgtgc
      781 gacgcacttt agaaatgcgt cgcacattac aaggagacaa tctatgtacg atctaatcat
      841 cattggtggc gggccagccg gattaactgc cacgatctac gcactccgca aacgactgaa
      901 tgtgctgcta atctccaaag acctcggcgg caaaaccaat tttcgattgc agctgccgga
      961 agtagaacac catttggtaa tcaacggcga agaagtcgtc agccgcttcg tcaacgaaat
     1021 tcgctatctc gatttctcgc gcatcatgga taaagtcgag aagatcgaaa agatcgatcg
     1081 cggctatcgc gtgttcaccc gcggcggcaa agagcatgag cggccattgg gcgaatacga
     1141 cacccgatcg atcatcatcg ccagcggcac gcagggacaa ttcctgaatg tgcccggcga
     1201 gaaagattac ttgatgcgcg gcttgtgctt cagtgccatg agctatgcgc cgctattcat
     1261 cgagcgcgac accgtcgtcg tgggcgatga cgatctggcg atgcgcgccg cgatggaact
     1321 ggcgcagatc tgcaagagcg tgacgctgat cgcgccgacg cacggcaagt tggacagccc
     1381 gattggcaag cggctgcaaa catggccgaa tgtcttgatt ctggaaggct atcaagccaa
     1441 gcaggttaaa ggcgactcgt atgcacgcag tttagtcatc ggcaagaacg gcgacacccg
     1501 cgaactgaag gctgatgcga tcttcgtcga actgggcttg aagccgaatt cggaaatggt
     1561 gaagggtctg gtcgatctgg aagccgatgg ccggatcagg atcgatgcgc agaatcgcac
     1621 gtccgcgccg ggcattttcg cggcggggga tgtgaccaac gcgtacgccg agcaagtgct
     1681 gatcgcgatc ggtgaaggcg cgaaagccgc cttagcggta catgaatatt tgatggagca
     1741 gcccatcgaa cagaaagcaa tggccgagtt gtggcggtaa ttttagaaat caggtttcat
     1801 tgagaggcct gattactttc ttgaagtcgt gaagtccact cacctcatcc cgcttgcgct
     1861 cgatggggtg agattatatt ttgagcagga atacaaatgg agttcccatg tccaagattg
     1921 atactctcgt gcaagacttt ctcgcgcaga aaaagatcgc cgtggtcggt gtttcggata
     1981 agcgcgacac cggctgcaat ctcgcttatc gaaagttcaa agccgcaggc tataccgtct
     2041 acggcgttaa tccacgcatc accacctttg aaggcgatcc catctatccg gatttgaaag
     2101 caattcccga tcggctggaa gcggtattca ttctcaccaa tcccaaagtc accgatcaaa
     2161 tcgtgcagca gtgcgtcgag ctcggcatca agcacgtgtg gatgcactgc atgatgggca
     2221 cgaaacccgg tttagtggcg ggcatgacca gcgtctcacc agacgcggtg cgaatctgcc
     2281 gcgagaacgg gatcgcggtg atccccggca cctgtccgaa ccagttcttg gaggctgatt
     2341 tcttccatgg gctgatgcgc aagatgtggc gcgtgttcgg ctttctcaag atcagcgaca
     2401 atggatcgat caaagcgaag gcttgagact gcagcctcca gcacagttca ctatgaagaa
     2461 aatcctcgtg acttacgcca ccttcgccgg ttcgaccgtc gaggtagccc aagcgatcgg
     2521 tgacgagatc accaagagtg gctggcaggt ggatgttctg cccatcggtg aggtgaaaga
     2581 tgttgaggct tatgcgggcg tcgtcctcgg cgcaccgatg atcgttggct ggcatcgcgc
     2641 cgcgctaaca ttcctcaagc gacatcgatc ggtgttgcag cacagtccac tggcgatctt
     2701 cgtcatggca atgagcctga cccagactga cgaaacgtgc atcgatggtg tgcccgtatt
     2761 tgtcgacgag aaactgccga aaccgagcga aaaagcaggg cacttgagtt tcagagaacg
     2821 ccataccagt ctggcgaatt acgttccacc tattctcaag gccacccgcc cgggcaaacc
     2881 cgtgagcgtg ggcgtctttg gtagccgctt ggaatatcgg cgcttgaagt ggtgggccat
     2941 gctgttcgtc atgttgatcg ttcaagcccc ggctcgcgat cggcgaaatt ggacggcaat
     3001 tcgatcgtgg gcagctgggc tggcggcggc actccaactt gaggagctag aactacaatt
     3061 gactaccggc gcgcaaaact gaatagttcg ctttacaaat atgaaacggt cggctacact
     3121 tcaggcgtaa tgcgtaaaac ctgaatcgtt ttgaaaggct cgatgagcaa tgagtaacga
     3181 taatccttca accaatccac cggccaacac gcctccttcg aatcaagatt ggcgagacgt
     3241 gcggcgtgcc gaacgagacg agcgccgcgc ttggcgtcga tcaggcggcg gagcatggat
     3301 cggcggcgca gtcttgatcg cgttgggc
//
LOCUS       DASRTJ010000169         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_344697,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000169 DASRTJ010000000
VERSION     DASRTJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344697"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..417)
                     /locus_tag="VFF70_03615"
     CDS             complement(22..417)
                     /locus_tag="VFF70_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43814.1"
                     /translation="MIQRRSISWGSLIGGLSLIILGLVLLIDRSTNFLPDFIQRYWAI
                     LLIADGVWNMIARLRFKEDAWAGMDYGTGLYVIRHRRQQRTRWWVAIVLLIAGAFCLW
                     ASLTPSANITAGPIITIILGVLFVLRSFL"
     gene            complement(539..916)
                     /locus_tag="VFF70_03620"
     CDS             complement(539..916)
                     /locus_tag="VFF70_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HZY43815.1"
                     /translation="MSTVEQAINKFFAAFERADESASVKAVYGEPIEYEEKIVLPIAT
                     ITHGFGLGGGMAADKKSAEDLGAGGGGGGYVGAQPVALAEISADGVKVIPIIDENRAL
                     AAGLAFAVWAVYWTARTLMKIFK"
     gene            complement(955..1200)
                     /locus_tag="VFF70_03625"
     CDS             complement(955..1200)
                     /locus_tag="VFF70_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43816.1"
                     /translation="MISWQMKDAIIGSTATVPMWGWSVTHLRPTALIDRSAGEERRIK
                     IVNHTQQIELILLIAAVLLPIVLNAVASLARRHRTNS"
     gene            complement(1280..2584)
                     /locus_tag="VFF70_03630"
     CDS             complement(1280..2584)
                     /locus_tag="VFF70_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43817.1"
                     /translation="MNTRLAKKLRAALAKSDRSFIDQVTALATQSKRPLYLVGGPVRD
                     LLLGQSIIDFDLTVEGDAIDLAQQLAKKLRGSIKTHDRFGTAKLILPTQSASTASRSG
                     ASVDLASTRSETYAQPGALPDVTPGNLQSDLIRRDFSINAMAIRLDGDHFGELIDMYQ
                     GADDLKRGLVRVLHPQSFTDDPTRIFRAARFTERFNFKVEAKTLKLIPAALPIIDRVS
                     GDRLRREFEAIFKEDQPDRALALLDQWGVLKQIDPEFHLDAEKRLRFRKAAPPFDSLT
                     GWVWLLRRVSLEGRARIAQRLNLLVEDSTDLNQVYEIQGQQAAIKDSPAPSVLYQLLS
                     RYSDRALRTATTIIDDDRANRKIDHFVNDLHGVKISIDGQRLQEFGLVPGPAMRRVLE
                     SIRDAVLDGLIVSPQRVEEFAKILIAREQEGNKPGFVQKPWL"
BASE COUNT          608 a          738 c          710 g          597 t
ORIGIN      
        1 acatcactca aggctgttga tttacaggaa acttctgagg acaaacagga caccgaggat
       61 gatcgtaatg atcggaccgg cggtgatatt tgcggacggc gtaaggctcg cccacaaaca
      121 aaacgcgccg gcaatcagca gtacgatcgc cacccaccac ctcgtgcgct gctgtcggcg
      181 gtgccgaata acgtataagc cggtgccata atccatgccg gcccatgcgt cttctttgaa
      241 gcgcagccgc gcgatcatat tccacacgcc atcggcgatc agtaagatcg cccaatagcg
      301 ttgaatgaaa tccggcaaaa aattggtgga tcgatcgatg agcaggacca accctaatat
      361 gatcaggctt agtcctccga tcagcgatcc ccatgatatc gatcgacgtt gaatcatgat
      421 gtctacattt catgtcattc cgagcggagc gcggaatctc tacgttgatc aacaacgcta
      481 gcgaattgaa cctgagatcc ctcgccacac aacgctacgg ctcggaatga ccttgcggtc
      541 acttaaatat tttcatcagc gtgcgtgcgg tccaatacac cgcccacacc gcaaaagcta
      601 gaccggcagc caacgcacgg ttctcatcaa tgatcggtat aactttgacg ccgtccgcgc
      661 tgatctctgc caatgcaacg ggctgtgcgc ccacataacc gccccctccg ccgcccgcgc
      721 cgagatcttc tgcggatttt ttatctgcgg ccattccacc gcccaggcca aagccatgcg
      781 taatcgttgc aatgggcagg acgattttct cttcatattc aatcggttcg ccatagacag
      841 ccttgaccga cgcgctctca tcggcacgtt cgaaagcggc aaagaatttg ttgatggcct
      901 gttcgacagt agacatatgc ggactcctga tcgggacgtt ccaccggaac atctctatga
      961 atttgttcga tggcggcgcg ccagcgaggc aactgcattt aagacgattg gtaaaagtac
     1021 ggcggcgatc aacaagatca attcgatctg ctgagtgtga ttgacgatct tgatccggcg
     1081 ctcttcacca gccgatcgat cgatcagcgc cgtcgggcgc aggtgcgtga ccgaccagcc
     1141 ccacatcggc acagtggcgg tgctgccgat gatcgcgtct ttcatctgcc acgaaatcac
     1201 ttcggcttca ggcgtgactt catgcgcgcc aactttgatc ggtttactgc gaacgatttt
     1261 gcgtttcagc ccagcgaggt caaagccagg gtttctgcac gaaacccggt ttgttcccct
     1321 cctgctcacg tgcgatcaaa atcttggcga attcttcaac ccgctgcgga ctcacgatca
     1381 ggccatcgag tacggcatcg cggatcgatt ccaggacgcg acgcatagcc gggccgggca
     1441 cgagtccaaa ttcttgcaag cgctggccgt cgatcgagat tttcacgccg tgtagatcgt
     1501 tgacgaagtg atcgatcttg cggttggcgc ggtcgtcgtc gatgatcgtc gtggccgtgc
     1561 gcagcgctcg gtcggagtaa cgactcaaga gctgatagag tacgctgggc gctggagaat
     1621 ctttaatcgc ggcctgctgc ccttgaattt cataaacttg attgaggtca gtgctatctt
     1681 caactagcaa gttcaggcgc tgcgcaatgc gcgctcgacc ttcaagtgac acgcgccgca
     1741 gcaaccacac ccagccagtg agtgaatcaa aaggcggtgc tgcttttcga aaacgcagcc
     1801 gcttttccgc atcgaggtgg aattccggat cgatttgctt caacacgccc cattgatcga
     1861 gtagggccag cgccctatcc ggttgatctt ctttgaagat tgcttcaaat tcgcggcgca
     1921 agcgatcacc gctgacgcga tcgatgatcg gcagggcggc gggaatgagt ttcaatgttt
     1981 tggcttccac tttgaagttg aagcgttcgg taaaacgcgc cgcgcgaaag atgcgcgtgg
     2041 gatcgtccgt aaaactttga ggatgaagca ctcgcactaa accgcgcttc aaatcatcag
     2101 ccccttgata catatcgatc agttcgccga agtgatcgcc gtcgaggcgg attgccatgg
     2161 cgttgatcga aaaatcgcgg cgaatgaggt cactttgaag attgccggga gtcacatcgg
     2221 gcagcgcgcc cggctgcgca taagtttccg accgcgtaga cgccagatcg acacttgccc
     2281 cactgcgtga cgcagtgcta gccgattggg ttggtaggat caatttcgca gtgccaaatc
     2341 gatcatgggt tttgatcgag ccgcgcaatt ttttcgccag ctgctgcgcc agatcgatcg
     2401 catcaccttc gacggttaaa tcgaaatcta taatggattg accgagcaat agatcgcgca
     2461 ccgggccgcc gacgagataa agcgggcgtt ttgattgggt ggcgagtgca gtcacttgat
     2521 cgatgaagga tcgatcggat ttagccagag ctgcgcgtaa ttttttcgcc agacgagtgt
     2581 tcatcgctgc gattatacac ctcatcgcgc cgatgatcag atcgaacagt taaggcgtcg
     2641 gaatgatcga ttg
//
LOCUS       DASRTJ010000170         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_193359,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000170 DASRTJ010000000
VERSION     DASRTJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193359"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFF70_03635"
     CDS             complement(<1..607)
                     /locus_tag="VFF70_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZY43818.1"
                     /translation="MSDLALFSELTILSLEQATTLPFLTQRLARDGARVIRIEAPGRG
                     DPNRYVGRNLLNEDGMASYFLPNNCGKQAITLNLAEAEGRSILHQLIDHLRVDIFATN
                     NRPSSYQKLGIDYDTLKAIKSDIIWLGITGFGPQSDEAAYDPVLQARSGWMALTGKPD
                     DDPLVFGLPMVDLGAAEHAYGAVMKALYRRALSPLSREAGEG"
     gene            complement(629..1474)
                     /locus_tag="VFF70_03640"
     CDS             complement(629..1474)
                     /locus_tag="VFF70_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZY43819.1"
                     /translation="MTSHQHEIELPDLRIVPIADLMLHEQHDAQRSIPLAMRLQAEGV
                     LKSPPIVAPIVDDCRFVVLDGANRVTALSELGLEHIAVQVVDYEDPELVLDTWSHLIS
                     GFPCDEFSRLIGLLVGVQIEAADLIRARAELARRQALVYFSLPGDGVYTVCVEGELDY
                     RTEVLNQIVDIYKSRGIIYRANTDHLDQLLPDHDDVTALVVFPRYEPAEIIELARVGV
                     RLPAGITRHIIPRRALRINFPLALLQDARPLTEKNRSLQHWLKVKAANKEVRFYQEST
                     YLFDE"
     gene            complement(1515..3113)
                     /locus_tag="VFF70_03645"
     CDS             complement(1515..3113)
                     /locus_tag="VFF70_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZY43820.1"
                     /translation="MPDKIRDVILESGIAVKPVYGPDDLDPAREIGQPGEFPFTRGIH
                     PLMYRARPWTMRQYAGFGTPSETNERFKFLIGNGQNALNVAFDLPTQMGLDSDDPLAE
                     GEIGRVGMAVDTLADMEVAFDGIPIDQISVSLTINAVAAPIMAMYFVVAEKHGVQLSD
                     VRGTAQNDILKEYIGRGAWIFPVEHGIRLIGDTIEFCAHHAPKYYPVSVCGYHIRESG
                     ANPVQEIAYAFCIARAYIRLGLERGLNVDDFAERLSFNFDIFGNLFEQVAKFRAARRL
                     WATLMQAEFGAKKPGTLQMKMIAGGGGGGLTIEQPENNIVRGAYYALISALSGAQTMA
                     LCSYDEAYTIPTEKAALISLRTMQILVEEMGLCDTVDPLGGSYYVETLTNEMEQRIVQ
                     EIKRVDQEMGGIVRAVSDGAVQREVAKQAWKVEQGLQNGSIPKVGVNRYRLDDSQETR
                     DVELHSYQPAQATESIRRTNEVRANRDQPAVAIALEKVRDAAINNQNVMPAMMGAVRA
                     YATLGEITQTLKGVFGEFKEPVRL"
BASE COUNT          704 a          977 c          896 g          698 t
ORIGIN      
        1 gcccctcccc cgcttcgcgt gagaggggag acagagcgcg tcgatatagc gccttcatca
       61 cggcgccata tgcatgttcc gccgcaccga gatcgaccat cggcagcccg aagaccagcg
      121 gatcatcatc gggcttgccc gtcagcgcca tccagcccga tcgggcttgc agcaccggat
      181 cgtacgcggc ttcatcggac tgcgggccga agccggtgat gccgagccag atgatgtcgg
      241 acttgattgc cttcaatgtg tcgtagtcga tgcctagctt ctgataacta gagggacgat
      301 tgttcgtcgc aaatatatcg acgcgcaagt gatcgatcag ttgatgcaaa atcgatcggc
      361 cttctgcctc agcgagattg agcgtgattg cctgcttgcc gcagttgttg ggcaagaaat
      421 aactcgccat gccgtcttca ttcaacaggt tgcgaccaac atagcgattg ggatcgcccc
      481 gccccggcgc ttcgatgcgg atcacacgcg caccatcgcg cgccaggcgc tgcgtgagaa
      541 acggcagcgt ggtggcttgt tcgagcgaga gaatggtcaa ctctgagaat aacgctagat
      601 cactcatgag cgtgtcgatc gaatgacttc attcgtcaaa caggtaagtc gattcttgat
      661 agaagcgcac ttccttgttc gcggctttca ctttcaacca atgctgcaag gaccggtttt
      721 tttcggttag cggccgagca tcctgcaaaa gcgccaatgg aaaattgatc cgcagcgcgc
      781 ggcgggggat aatgtggcgc gtgatgccgg ccggcaggcg aacaccgaca cgcgccagtt
      841 cgataatctc cgccggctca taccgcggaa agactacgag ggccgtcaca tcatcgtggt
      901 cgggcagcag ctgatcgaga tggtcggtgt tggcccgata gatgatgccg cgcgatttat
      961 agatatccac gatctggttc agcacttcgg tgcggtaatc gagctcgccc tcgacacaca
     1021 cggtatagac cccatccccc ggcagcgaaa aataaaccag cgcttgacgc cgggccaatt
     1081 cagcccgtgc acggatcaag tcggccgcct cgatttgcac gccgaccagc agtccgatca
     1141 agcgactaaa ctcatcgcag ggaaagccgc taatcaaatg ggaccaggta tccaatacca
     1201 attcgggatc ttcgtaatca acgacctgaa cggcgatgtg ttccagaccc aattccgata
     1261 acgcggtaac gcgattggct ccatccagca ccacaaagcg gcaatcatcc acaatcgggg
     1321 cgacgatggg gggactcttc agcaccccct ctgcctgaag tcgcattgcc agcgggatac
     1381 tgcgctgtgc atcgtgctgc tcgtgcagca ttagatcggc gatcggcaca atccgcagat
     1441 cgggcaactc gatttcgtgt tgatgactcg tcatgggaaa atgaccacgc gatctgggcg
     1501 caaatccaag tcctttataa cctgaccggt tctttaaatt cgccgaacac acccttcaag
     1561 gtctgggtga tctcgcccaa cgtggcatac gcgcgaacgg cgcccatcat ggcgggcatc
     1621 acgttttgat tattgatcgc ggcatcgcgt actttttcca acgcgatcgc gacggctggt
     1681 tgatcgcgat tagcgcgaac ttcgttcgta cgtcggattg attcggtggc ttgtgccggt
     1741 tggtaggaat gtaattcaac gtcgcgcgtt tcttgcgaat catccagccg gtaacgattc
     1801 acgccgacct tggggataga tccgttttgc agtccctgct caaccttcca agcttgtttc
     1861 gccacttcgc gttgcaccgc accatcgctg actgcgcgca cgataccgcc catctcctga
     1921 tcgacgcgct ttatttcctg gacgatgcgc tgttccattt cgttggtcag cgtttccaca
     1981 taataggatc cgccgagagg atcgacggtg tcgcacagtc ccatctcttc gacgaggatt
     2041 tgcatcgtgc gtaaagagat caacgccgcc ttctcggtgg ggatagtgta ggcttcatca
     2101 tacgaacaca aggccatcgt ctgcgcgccg gacaaagccg agatgagcgc gtagtaggcg
     2161 ccgcgcacaa tgttgttctc cggctgctcg atcgtgagtc cgccaccgcc gccgcccgcg
     2221 atcatcttca tttgcagggt gcccggcttt ttcgcgccga attcagcctg catgagggtg
     2281 gcccacaagc gccgcgccgc gcggaacttg gccacctgct cgaataagtt gccgaagata
     2341 tcgaagttga aggagagccg ctcggcaaaa tcatcgacgt tcaagccgcg ctcgagaccc
     2401 aatcggatat aagcccgcgc gatacaaaac gcatacgcaa tctcttgcac cggattcgca
     2461 cccgactcgc ggatgtgata gccgcagacg ctgactggat aatatttcgg cgcatgatgc
     2521 gcacagaact cgatcgtgtc gccgatcagg cggatgccgt gctcgaccgg aaagatccac
     2581 gcgccgcgtc caatatattc tttgagtatg tcattctgag ccgtgccacg cacatctgac
     2641 agctggacgc catgcttttc cgcgacgacg aaatacatcg ccatgatcgg cgcggccact
     2701 gcattgatcg tgagtgacac gctgatctga tcgatcggga tgccgtcgaa ggccacttcc
     2761 atatccgcca gcgtatcgac cgccatgccg acccgcccaa tttcaccttc agccagggga
     2821 tcgtccgaat cgagtcccat ctgcgtgggc agatcgaacg cgacgttgag cgcgttctgt
     2881 ccgttgccga tcaaaaactt gaagcgctcg ttcgtttcgc tcggcgtacc gaagcccgca
     2941 tactgccgca tcgtccacgg gcgggcgcgg tacattaacg gatgaatgcc gcgggtaaat
     3001 gggaactcgc cgggctgccc gatttcacgc gccggatcga gatcgtcagg gccatacacc
     3061 ggcttgacgg caatgcctga ttctaagatc acgtcacgaa ttttgtctgg cataactcac
     3121 gccaccgttt ctttcctagc cactcgttcg cggatgaaag cggctattgt gtcaaactca
     3181 ctgctggtag gaaacacgcc cgcgacgcct aatgctttaa gcgcagctgc atctcgatcg
     3241 ggtatgttgc ccccgacgag cacaagagcg tcatc
//
LOCUS       DASRTJ010000171         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_9762,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000171 DASRTJ010000000
VERSION     DASRTJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9762"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..552)
                     /locus_tag="VFF70_03650"
     CDS             complement(154..552)
                     /locus_tag="VFF70_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZY43821.1"
                     /translation="MEITVKQEEGRRPISVIHVKGDIDSNTYQQLQAEIDKSIAAGIH
                     NMLIDMSEVPFMSSAGIRVLNHTFNELRGTLPQETDEAMKKGLRDGTFKSPHLKILNP
                     SARVLEVLKMAGVDMFLEIHKDYSEAVASY"
     gene            complement(695..2434)
                     /gene="recN"
                     /locus_tag="VFF70_03655"
     CDS             complement(695..2434)
                     /gene="recN"
                     /locus_tag="VFF70_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZY43822.1"
                     /translation="MLAELTITDFAIIDKLHLDIASGFNVLTGETGAGKSIVIDAVSL
                     LLGGKAGNEMVRAGTERALIDGVFRINSMAREAIRSILQANELLDEDDPDMIIISREI
                     RSGGRSTCRINGRAVPVGLLKDVGELLVDIHGQSEHLSLLRPREHVNLLDRYANTWDL
                     RQKYSAGVTELREVRKELSELLKSEAEIARRADMLKFQIEEIKSAKLKAGEDEELTSL
                     RNRLANAESLAALADDAYTSLYEGAGETPAALDALAHAQKALSGLAKIDPKFQEHVNT
                     IDTAVAQVDDLAREVKSYRDAIEYSPKRLETAEERLDLIKRLKRKYGAAIAEVISFAD
                     RATKELDGIEHSGERIEELRQQEDRLLHEIGLAGHELSGARTAAAEQLSKSIEVELND
                     LRMSGAHFAVEQKWEPEANGAFANDGRYAFDATGLDRVEFLVAPNVGEGLKPLVKIAS
                     GGETARLMLALKTVLSRADQTPTLIFDEIDQGIGGRVGTTVGQKLWGLSQHHQVVCIT
                     HLPQLAGYGDTHFKVEKRINGERTLTHITALTDQARVEELAQMLGASGEGATLSAQEI
                     LNSVAVVKQQAGR"
     gene            complement(2500..>2909)
                     /locus_tag="VFF70_03660"
     CDS             complement(2500..>2909)
                     /locus_tag="VFF70_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43823.1"
                     /translation="CVHAQQGIFYNALPTDYLITAAITLPIAYVAALIVPRLFWLAIF
                     AGPIVGGIISEGIRIATKKRRGRYIWAVAVGCLIVATLVALLPVLDVMFSGRLGLVPG
                     IASTVSLDLLIDGIYLVLCGGTLIARLRFGSKM"
BASE COUNT          659 a          900 c          675 g          675 t
ORIGIN      
        1 atcgtgttcg ttgccatttc gcgcctcctc ctttaacaat tatagcactc tgcttaaaaa
       61 cagccgcgcc aaacaaaaac gcggattcag atagaatccg cgtttacaca gccaagtgat
      121 ccattccatt cagctaatcg aaaggatctt gggttaataa gacgcaaccg cctcgctata
      181 atccttatga atctccagga acatgtcgac gccggccatc ttcaagactt ccaatacccg
      241 cgccgacgga ttcaatatct tcagatgagg cgacttaaac gtcccgtcgc gcaggccctt
      301 cttcatcgct tcgtcggtct cttgcggcag cgtgccgcgc aattcattga aggtgtgatt
      361 caacacgcga atgcccgcgc tgctcatgaa cggcacttcg ctcatatcga ttagcatgtt
      421 gtgaatgccc gccgcgatcg acttatcgat ctcggcttgc agctgctgat acgtgttgga
      481 atcgatgtca cctttgacgt gaatcacgct gattggtcgc cggccttctt cctgcttaac
      541 tgtgatttcc attttatttt ctcccgtttg ccatgatgtt gaatttggtt tatgccagac
      601 aatttacgtt tgtcatgctg agtcccgcag ggacgaagca tctcggaaaa ttgtgcgaga
      661 cccttcgctt cgctcagggt gacaacctag aagtttatcg acccgcctgc tgcttgacga
      721 cagccacact gttcaaaatt tcctgcgcgc ttaatgttgc gccttcaccg ctggcaccga
      781 gcatctgcgc cagctcttca actcgcgcct gatcggtgag cgctgtaata tgcgtcaggg
      841 tacgctcacc gttaatccgc ttctctactt tgaaatgcgt gtcgccgtaa ccggccaact
      901 gcggcagatg cgtaatgcac accacttgat ggtgttgact caaaccccac aacttttgtc
      961 ccaccgtcgt ccccactcgc ccgccaatac cctgatcgat ttcgtcaaag atcaacgtcg
     1021 gcgtttgatc ggcgcgcgac aagactgtct ttaacgccaa catcaaacgc gccgtctcgc
     1081 cgccagaggc aatcttcacc agcggtttca gcccctcgcc gacattcggc gccaccagga
     1141 attctacacg atctaacccc gtcgcatcaa atgcgtaacg gccatcgttg gcaaacgcgc
     1201 cattcgcctc cggctcccat ttctgttcga cggcaaagtg cgcgccactc atgcgcagat
     1261 cattgagttc gacttcgatc gatttcgata actgctccgc ggcggctgtg cgcgcgcccg
     1321 atagttcatg gccggccaga ccgatctcgt gcagtaaacg atcctcttgc tggcgcagtt
     1381 cctctattcg ctcaccggaa tgttcgatgc catccagttc tttcgtcgcc cgatcggcaa
     1441 agctgatcac ctcggcaatc gccgcgccgt acttgcgttt cagccgcttg attaaatcca
     1501 ggcgctcttc agccgtctcc aagcgcttgg gcgaatactc gatcgcgtcc cgataactct
     1561 tgacttcgcg cgccagatcg tcgacctggg ccaccgccgt atcgatcgta ttgacgtgct
     1621 cttggaactt aggatcgatc ttcgccaaac cgctgagcgc cttctgggca tgcgctaaag
     1681 catcgagcgc cgctggagtt tcgcccgccc cttcatataa agaggtgtaa gcatcatcgg
     1741 ccagcgcagc caatgattcg gcgttcgcta atcgattgcg caatgacgtc aattcttcgt
     1801 cttcacccgc ttttaatttc gcggacttga tctcctcaat ctggaacttc agcatatcgg
     1861 cgcgccgcgc gatctcggct tcgctcttta acaactcact caactctttg cgaacttcgc
     1921 gcaattcagt cacgcctgct gaatattttt gccgcagatc ccaagtgttg gcatatcgat
     1981 cgagcaggtt gacatgctca cgcggccgca gcagcgaaag atgctcgctc tgcccgtgaa
     2041 tatcgaccag cagttcgcca acatccttga gcaaacctac cggcacggcc cggccattaa
     2101 tgcggcacgt cgatcggccg cccgatcgga tctcgcgcga aatgatgatc atgtcgggat
     2161 catcttcgtc cagcaattca ttcgcttgca agatcgatcg gatggcttca cgcgccatcg
     2221 aattgatccg gaacacgcca tcgatcaacg cgcgctcagt accggcccgc accatttcgt
     2281 tgccagcctt gccacccagc aacaagctga ccgcatcgat cacaatcgat ttgcccgcgc
     2341 ccgtttcgcc ggtcaagaca ttaaagcccg acgcaatgtc caaatgtagt ttatcgataa
     2401 tcgcaaaatc ggtaatcgtt aattcagcta gcatacgcgc tcgattgatg gtaaagcgcc
     2461 gcaggcgcgt accatcatta ttccggctaa aacgtgaatt tacattttgc tgccaaaccg
     2521 cagacgcgcg atcagcgtac caccacataa caccaagtag atgccgtcga tgagcaaatc
     2581 gagcgacacg gttgaggcga ttcccggaac caagccaagc cgcccgctga acatcacatc
     2641 caagaccggc aatagagcca ccaacgtagc aacgatcaaa catccaacgg ccacggccca
     2701 aatgtaacgc ccgcgtcgtt tcttggtggc aatgcggatc ccttcagaaa taatgccgcc
     2761 gacaatcgga ccggcaaaga tcgccagcca gaacaatcgg ggcacgatca aggccgccac
     2821 ataggcaatc ggcaaagtga tagccgccgt aatcaagtaa tcagtcggca gggcattgta
     2881 aaagatgcct tgctgggcat gcacacaat
//
LOCUS       DASRTJ010000172         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269237,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000172 DASRTJ010000000
VERSION     DASRTJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269237"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2245
                     /locus_tag="VFF70_03665"
     CDS             <1..2245
                     /locus_tag="VFF70_03665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZY43824.1"
                     /translation="GVSAVPGSGKTAILSYLAARLVREKVRGDEEVLIVTFANSAVDN
                     FSRRIKEFLQDTSEHTGSPLLPEVGYRVRTLHGLAHDILRERPALLGLPEDFSIIDER
                     VSDELINDIVANWIKSNTIVFEAFVDASIDESRRKWLLREKWPDLLKSIARSFIRRAK
                     DLQMTPRQVRDGLPSTSGAWLIGKLGADIYEDYERALSFRGGIDFDDLIRLALLGLQT
                     DPQLLARLQARWPYILEDEAQDSSELQERILRLLTHSGNWVRVGDPNQSINTTFTTSD
                     IRYLVEFLTEADVEERPIIHSGRSNQRIIDLANYLVDWTIESHPNDTIRASAFRSQHV
                     EPTPPGDPQPNPIDDASDVVKLYNKALNPDQELDLVVKSIGRWLPDHQDRTVAVLVPD
                     NQRGFKIAEQLKANKIPCDELLRSTSGTRAAARALSDVLRYLAAPLSGPLLSKAYEAV
                     FKRADDNEESDQIALDDLHKTIGGGLRHCEKVETYLWPRPDLDWLAEYKPAAEDQIIR
                     SELIDFRKTVTLWLDAAAQLPIDQLILTVAQDLYRSDDLDRAARDLALAHKIALVLRQ
                     IANEHSEYRLPDLASELDRVANNEQRFIGFGEDDLGFNPRAGRVTVATMHKAKGLEWD
                     RVYLLGVNNYDFPSAESHDVYRGETWYIRDKLNLEAEAIGQVEAINKRSSYLIGIATE
                     KARIDYVAERLRLLYVGITRAKRELILTWNTGSFENEPKQPAAPFIALYTFMEKNAGM
                     RGGGKER"
     gene            2294..>2613
                     /locus_tag="VFF70_03670"
     CDS             2294..>2613
                     /locus_tag="VFF70_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZY43825.1"
                     /translation="MPLPTNFQFSQASLSDYVDCARRFQLRYLLEQDWPAVESEPLLE
                     RERFVDLGRRFHKLIQQHAEGISIDALMQSANEPELARWWPNYLATLKTNLNDLPNDR
                     RAEVA"
BASE COUNT          615 a          742 c          695 g          561 t
ORIGIN      
        1 gggcgtctcc gccgtgcccg gcagcggcaa gaccgcgatt ctgtcttatc tggccgcccg
       61 gcttgtgcgc gaaaaagttc gcggcgatga agaagtcctg atcgtcacct tcgccaattc
      121 cgccgtcgat aatttttcac gccgtatcaa ggaattcctt caagatacca gcgagcacac
      181 cggctcgccc ctgctgcctg aggtcggcta tcgcgtgcgc acgctgcatg gcctggcgca
      241 cgacatcctg cgcgaacgcc ctgccctgct cggcctgcct gaagattttt cgatcatcga
      301 tgagcgcgta tccgacgagc tgatcaacga cattgtcgcc aattggatca aatcgaatac
      361 gatcgtcttc gaggccttcg tcgatgcctc gatcgatgag agccgccgca aatggttgtt
      421 gcgtgagaag tggccggact tattgaagtc tattgctcga tcgtttattc ggcgcgccaa
      481 agatttgcaa atgactccac gccaagtgcg cgacggactg ccatcgactt ctggcgcgtg
      541 gttaatcggc aagctaggcg cagatattta cgaagattac gaacgcgcct tatcatttcg
      601 cggcggcatc gacttcgatg atctcattcg cttggcgctg ctcggcttgc aaaccgatcc
      661 gcagctgctc gcgcgtttgc aagcacgctg gccgtacatc ctcgaagacg aagcacagga
      721 ttcaagtgag ctgcaggaaa gaattctgcg attgctcacc cacagtggca actgggtgcg
      781 cgtcggtgat ccgaatcaat cgatcaacac caccttcact acttccgaca tccgttatct
      841 ggtcgaattc ctgactgaag ccgatgttga agaacgcccg atcattcact ccggccgatc
      901 gaatcagcgc atcatcgatc tcgcaaatta cttggttgat tggacgatcg agtcacatcc
      961 caatgacacg atccgcgcca gcgcctttcg atcgcagcac gtcgaaccga caccacccgg
     1021 cgatccgcag cccaacccga tcgatgacgc gagcgacgtc gtgaagctct ataacaaagc
     1081 actgaatcca gatcaggagc tcgatctcgt cgtcaaatcg atcgggcgct ggctgcccga
     1141 tcatcaagat cgaacggtag ctgtgcttgt gccggataat caacgcggct tcaaaatcgc
     1201 cgagcagttg aaagccaata agattccttg tgatgaactg ctgcgcagca cgagcggtac
     1261 acgcgccgca gcccgcgctt tgagtgatgt gctgcgctat ttggctgcgc cgttgtccgg
     1321 gccattgctc tcaaaagcct atgaggctgt gtttaaacgc gccgatgata atgaagagag
     1381 cgatcaaatt gcgctcgatg atctgcacaa aacgatcggc ggcggcttgc gccactgtga
     1441 aaaagtggaa acgtatttgt ggccgcgccc cgatctggat tggctggccg aatataaacc
     1501 tgccgccgaa gatcagatca tccgatcgga gttgattgat ttccgcaaaa ccgtcacgct
     1561 ttggctggac gccgccgctc aactcccgat cgatcaactc attctcaccg tcgcgcaaga
     1621 tttgtatcga tcagatgatc ttgaccgagc cgcacgcgat ttggctttgg cgcacaagat
     1681 cgccctggta ttgcgacaaa ttgcgaatga acattcggaa tatcgattgc ccgatctcgc
     1741 cagcgaactc gatcgagtgg cgaacaatga gcagagattc atcggcttcg gcgaagatga
     1801 tctcggtttc aatccgcgag cggggcgcgt gactgtggcg acgatgcaca aggcgaaggg
     1861 gcttgaatgg gatcgcgtgt acttgctcgg cgtcaacaat tacgatttcc catcggcaga
     1921 gtcacacgat gtgtatcgcg gcgagacatg gtacattcga gataagctca atctggaagc
     1981 cgaagcaatc ggccaagtgg aagccattaa caaacgaagc agctatttga tcggcattgc
     2041 caccgagaaa gcgcggatcg attacgtggc cgagcgcctg cgcttattgt acgtcggcat
     2101 cacgcgcgcc aaacgggagt tgatcctgac ctggaacacc ggcagtttcg agaacgagcc
     2161 caagcagcct gctgcgccgt tcattgcgct ctatacattc atggaaaaga acgcgggaat
     2221 gagaggcgga gggaaagaga gataatagca aaccatcgct ctgtctttca ttccccccat
     2281 cgcccgatca aatatgccgt taccaacaaa cttccaattc agccaagcca gtctcagcga
     2341 ctatgtcgat tgcgcgcgac gctttcaact acgctacctg ctagagcaag actggcccgc
     2401 cgtcgagagc gaaccattac tcgaacgtga gcgtttcgtc gatcttggcc gccggtttca
     2461 caagttgatc caacagcatg ccgaaggcat ctcgatcgat gcgctgatgc aatcggcaaa
     2521 cgagcctgaa ctggcgcgct ggtggccgaa ttatttggca actctcaaaa caaatttaaa
     2581 tgacttaccc aatgatcgtc gggccgaagt ggc
//
LOCUS       DASRTJ010000173         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_9899,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000173 DASRTJ010000000
VERSION     DASRTJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9899"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..588)
                     /locus_tag="VFF70_03675"
     CDS             complement(67..588)
                     /locus_tag="VFF70_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43826.1"
                     /translation="MDELTHTFTGICLALDFRALLLTGSGAEEVAQKPNWKIAAQGDV
                     DLHLGVMLFDRPFVFTPRSIITERNQTIEGVANVLEWLGDGAYSMPRSEVFGLNARGI
                     ESQIFAREIDVEASPIALLGHGHWVMAQVEIDPDKGSEITPIGKLSLPVRFRRALKVY
                     RAGPRSNFEKMLD"
     gene            complement(667..2253)
                     /locus_tag="VFF70_03680"
     CDS             complement(667..2253)
                     /locus_tag="VFF70_03680"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HZY43827.1"
                     /translation="MGIQSKYGLEHHGLTPTKAVHWNLSPAELIEQAVKRGEGQLTKD
                     GPLLAITQPHTGRSPNDKYTVREASSEKEIWWDKNNALTEAQFDQLHQKTLAYLNGHD
                     LFVREMYACADPANRLCVRLIDAVAWTNLFANNLFIRPEAKDLADFTPDFTILHAPEL
                     KADPKADGTRSDTVITINFAKKIILIMGTRYAGEVKKSIFTILNYLMPKKGVLSMHCS
                     ANIGAKGDTALFFGLSGTGKTTLSADPDRGLIGDDEHGWGDSGVFNFEGGCYAKVIKL
                     SAEAEPEIFSTTKRFGTVLENVVIDPDTRALLLDLDTITENTRAAYPIDFIGNAVIPG
                     IGGHPKNVMFLTADAFGVLPPISKLTSEQAMYHFLSGYTAKLAGTERGVTEPQATFST
                     CFGAPFLPLHPAVYAKMLGQKLAKHGAQVWLVNTGWSGGPYGIGSRMKIAYTRAMVRA
                     ALNGDLDKVPTQIDPIFGLPMPTACPGVPAEVLNPRHTWADQAAYDQKARELAGLFVK
                     NFKQFEAQSRPEVIAVGPKI"
     gene            complement(2327..3628)
                     /gene="larA"
                     /locus_tag="VFF70_03685"
     CDS             complement(2327..3628)
                     /gene="larA"
                     /locus_tag="VFF70_03685"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZY43828.1"
                     /translation="MKLPIGWQLTTAEPDRSIKPIELVPATRAALAQPLHSKRLSELA
                     SATARVCIVFTDATRACPDHILVPAILRELEMAGVRDENITLLCATGLHRPSTPAEKI
                     AKLGQAVVDRYTVIDHDAQSAVILSEVKNLGQSGEALRFAQGDISLNPLLLQSDLILA
                     TGVVEPHQYAGYSGGGKTVVIGCGGEATISETHGPKYLDHPGVRLGKVEGNPFQQFVR
                     EAARSIGLKFVVNVVLDGDGQPVAVKAGDPIAVHDELIAIAKKLYEVPVPQQYDVVIA
                     KVDPPKDVNLYQASRAATAIGLSATPPIRLGGVIIVEAQCIERTGEGKGEVRFFEAMS
                     LSQNLDQVLSRFRLNGCRAGEQRAFMLAQVLKKYEVIIAGSECPDAVEACQMTAVNSI
                     DGAIDRIKHKIGKQASVLYLPHPLITLPIVMSNTSTGLRAS"
     gene            complement(3653..>4186)
                     /locus_tag="VFF70_03690"
     CDS             complement(3653..>4186)
                     /locus_tag="VFF70_03690"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955047.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZY43829.1"
                     /translation="KHSKLPKQRIFGQAGILDSARFAAFIALELKVSLENIQTAVMGG
                     HGDEMVPLLRYSTVFGMPLTEWLSKEKLDAIVERTRKGGGEIVNLLKTSAYYAPGAAL
                     AKMAIAVLTDQQLIVPCSTYLEGEYGLTDICFGVPVKLGRNGIEKIIELKLTDEEKAM
                     ADKSVAIIRDTMKALKM"
BASE COUNT          908 a         1245 c         1067 g          966 t
ORIGIN      
        1 cgtgagtgtc aaccaagtaa caacattttt gcggagaaaa agtaaggacg agcgatgtgc
       61 tcgtccttag tccaacattt tttcgaagtt cgatcgcggc ccggcgcgat aaactttgag
      121 ggctcggcga aaccgaacgg ggaggctcag tttcccgatg ggtgtgatct cactcccttt
      181 gtccggatcg atttcaactt gagccatgac ccagtggcca tgccccaaca gcgcgatcgg
      241 cgacgcttcg acatcgatct cgcgcgcaaa aatttgtgac tcaattccac gcgcgttcag
      301 gccgaacact tccgatcgag gcatgctata ggcgccatcg cccagccact ccagcacatt
      361 cgctacaccc tcgatcgttt gattgcgctc agtgatgatc gatcgcggtg tgaacacgaa
      421 cggccgatcg aataacatca cgccgagatg caagtccaca tccccctgcg cggcgatctt
      481 ccaattcggc ttctgtgcga cttcctctgc acccgaaccc gttaagagca gcgcgcgaaa
      541 gtcgagtgcg aggcaaatgc cagtgaaggt gtgcgtaagt tcgtccataa aatatagaag
      601 tcagatgcca gaatccagaa gaaagttatc gtctgaattc tggcttctct attctgaatt
      661 ctgcctttag attttcggcc ccaccgcgat cacttccggc ctggactgcg cctcgaactg
      721 cttgaagttc ttcacgaaca atccggcgag ttcacgcgcc ttctgatcat acgccgcttg
      781 atccgcccac gtatggcgcg gattcaacac ttcggctgga acgccggggc aagccgtcgg
      841 catcggcaaa ccgaagatcg gatcgatctg cgtcgggact ttatcgagat cgccgttcag
      901 tgcagcccgc accatcgcgc gggtgtaagc gatcttcatg cgtgagccaa tgccatacgg
      961 cccaccactc caaccggtat tgaccagcca cacttgtgcg ccgtgtttcg ctaatttttg
     1021 gccgagcatc ttggcataga ctgccggatg caagggcagg aacggtgcac cgaagcacgt
     1081 gctgaaagtt gcttgcggtt cagtcacacc gcgttcggtg ccggccaact tcgcggtata
     1141 gccgctcaag aaatggtaca tggcttgttc agaagtcaat tttgaaatgg gcggcagcac
     1201 accaaaagca tccgccgtca agaacatgac attcttcgga tgaccgccga tgccgggaat
     1261 caccgcatta ccgatgaagt cgatcgggta ggcggcgcgc gtgttctcgg tgatcgtatc
     1321 gagatcgagc agcagcgcac gcgtgtccgg atcgatcacg acgttttcca aaacggtgcc
     1381 aaagcgtttg gtcgtcgaga aaatttccgg ctcggcttcg gccgagagtt tgatcacttt
     1441 ggcgtaacag cccccttcga agttgaacac gccgctatcg ccccagccat gctcatcgtc
     1501 gccgatcaaa ccgcgatccg gatcagcgga cagcgtcgtc ttgcccgtgc cactcaagcc
     1561 gaagaacaag gccgtgtcac ccttcgcgcc aatgttggcc gagcagtgca ttgacagcac
     1621 gcccttcttc ggcatcagat aattcaaaat ggtaaagatc gacttcttga cttcgcccgc
     1681 gtagcgcgtg cccataatca aaatgatctt cttcgcaaaa ttgatcgtga tcaccgtgtc
     1741 cgaccgggtt ccatcggctt tgggatcagc ctttaactcc ggcgcatgca agatcgtaaa
     1801 atcgggcgtg aagtcggcca agtctttggc ttcgggccgg atgaataaat tgttcgcgaa
     1861 caagttcgtc caggctactg cgtcgatcaa acgcacgcac aatcgattgg ccggatcggc
     1921 gcaggcatac atctcgcgca caaagagatc gtgcccattc aaataagcca aggttttctg
     1981 gtgcaactga tcgaactgcg cttcagtcag cgcgttgttc ttgtcccacc aaatctcttt
     2041 ttcgctcgat gcttcacgca cggtgtactt gtcgttcggc gatcggccgg tgtgcggttg
     2101 ggtgatcgcc agcagcggcc cgtctttggt cagttgaccc tcgccgcgct tcaccgcctg
     2161 ctctatgagt tcggccggcg ataaattcca gtgcaccgct ttcgtcggtg tcagtccgtg
     2221 atgttcgagc ccatacttac tttgaattcc cattgtcgct cctgtttgca taccgcaaat
     2281 agtaagtggc ttatcgctag tcacctactc atgagccaca cctgcactat gacgctcgca
     2341 gtccagtgct tgtgttactc atgacgatag gtaaagtgat caacggatgc ggcaaatata
     2401 acaccgaggc ttgcttgccg attttgtgtt taattcgatc gatcgcccca tcgatcgaat
     2461 tgacggctgt catctggcag gcttcaacgg catcgggaca ctcgctgccc gcgatgatca
     2521 cttcatattt ctttagaact tgcgccagca tgaaggcgcg ctgctccccc gcgcggcagc
     2581 cattcagccg aaagcggctg agcacttgat caagattttg tgacagcgac atcgcctcga
     2641 agaatcttac ttctcctttg ccctccccgg tccgctcgat acactgcgct tcgacaatga
     2701 tcaccccgcc caatcggatc ggcggcgtgg cgctcaaccc aatggccgtc gccgcacggc
     2761 tggcttgata cagatttaca tctttcggcg gatcgacttt cgcaatcacc acatcgtatt
     2821 gctgcggcac aggcacttca tacaacttct ttgcaatcgc gatgagctca tcatgcaccg
     2881 cgattggatc gcctgccttc acagccaccg gctgaccatc gccgtccagc acaacattga
     2941 ctacaaattt caatccgatc gatcgcgcag cttcgcgcac aaattgttga aatggattgc
     3001 cctcgacttt gcctaagcgc acacccggat gatccaaata cttcggcccg tgcgtttcgc
     3061 taatcgtcgc ttcaccgccg cagccgatca ccaccgtctt gccgccgcct gaatagccag
     3121 catattgatg cggctcgacg acgccggtgg cgaggatgag atcgctttga agcagcaacg
     3181 gattgaggct aatgtcaccc tgagcgaagc gaagggcttc gcctgattgg ccgagattct
     3241 tcacttcgct cagaatgaca gcggattgtg catcatgatc gatgactgtg tatcgatcaa
     3301 caacagcttg ccccaacttc gcgatcttct cggcgggcgt gctcggtcgg tgcagaccgg
     3361 tagcacacaa gagtgtaatg ttctcatctc gcacgcccgc catttcgagt tcacgcaaaa
     3421 tcgccggaac caaaatgtga tcaggacacg cgcgtgtggc atcggtgaac acaatacata
     3481 cgcgcgcggt agctgaagcg agttcgctca agcgcttcga gtgtaatggt tgtgctagcg
     3541 ccgctcgggt ggccggcacc aattcgatcg gcttgatcga ccgatcgggt tcagcggttg
     3601 ttaactgcca gccaattggt aacttcactc gtcactcatc actcatcact aattacatct
     3661 tcaacgcttt catcgtatca cggatgatcg ccaccgactt atcggccatg gccttctctt
     3721 catcggttaa cttcaactcg atgatttttt cgatgccgtt gcggcctaat ttcaccggca
     3781 cgccgaagca aatatccgtg aggccgtatt cgccttcgag atacgtcgag cacggcacga
     3841 tcaactgctg atcggtcagc accgcaatcg ccatcttggc caaagccgca cccggcgcat
     3901 agtaggcgct ggtcttcaac aagttcacga tctcgccgcc gcccttgcgc gtgcgctcga
     3961 caatggcgtc cagcttttct ttcgacagcc attcggtcaa cggcatgcca aagacggtcg
     4021 agtaccgcag gagcgggacc atctcatcgc catgaccgcc catcaccgcc gtctgaatgt
     4081 tttcgagtga gaccttcagt tccagcgcga tgaacgcggc gaagcgcgca ctgtcgagaa
     4141 ttccggcttg accaaagatg cgctgcttcg gcagcttcga atgttt
//
LOCUS       DASRTJ010000174         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269313,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000174 DASRTJ010000000
VERSION     DASRTJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269313"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1414
                     /locus_tag="VFF70_03695"
     CDS             74..1414
                     /locus_tag="VFF70_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY43830.1"
                     /translation="MPESPFPQKSIAIIGAGAAGLAAAYDLAGAGHTVTVYEASPNVG
                     GLASGFKEPQWDWSIERFYHHWFQTDSSVLGLINEIGASDKVLFPRPITAIYYQGKFH
                     PFDSMFTNMPLFLLRHFPLKDVMRFGLAGAYLKFSSNWKSLEQVTADEWMTKRMGRNV
                     YESMWKPLLVGKFSEYYDLVNMAWMWARIHARTTRLGTFVGGFQAFFDTLADAVRQRG
                     ASIQLSTGVREIVSNETGGLKLFTPNGTLDYDACLVTTSPRLMSRLAPSLPPDYLSQL
                     SKLKSMGAVVLILSLTHQLSESGIYWHNLVKEAGFPFLAMCEHTNFVSPDHFGGDHLI
                     YCGDYLSPDHEYFKLTESELLDRFVPALSRFNPKFDRSWIKKVWLFREPYAQPVPPIN
                     HSQNIPDIRTPIDRLYFASMSQVYPWDRGTNFAVEIGRRTAKMIVSDFEQKQNT"
     gene            1513..2841
                     /locus_tag="VFF70_03700"
     CDS             1513..2841
                     /locus_tag="VFF70_03700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZY43831.1"
                     /translation="MKSITVVGVGYVGLVTGTCLADLGHRVVCLDVNAERVENLKKAI
                     LPIYEPGLEEIVRRNMNAGRLSFTTSYAEAINGASADAAAEFVFIAVGTPSAVDGKAD
                     LDYVRQAAESIAEAMDHPLIVVNKSTVPVGTGDWTADIIRSKQKRAVKFNVVSNPEFL
                     REGSAIYDFMQPDRIVLGSLEREAAEKVAQLYLPLRATLMITDLRTAEMIKYASNAFL
                     ATKISFINEIANICEELGADVKEVATGMGFDKRIGRAFLDAGVGYGGSCFPKDVLALA
                     HMAKERGRHPQLLQAVMDINADQRNHIVSKVRELAGGSVKGKKIGVLGLAFKPNTDDM
                     RDAPAIDIIKALESEGASILAYDPVAMSVAKQIMPNLKTVDNPYDAAAGCDALILMTE
                     WNEFKQLDFHKLKAAMKSPAMIDARNVYEPDKMAQAGFNYRGVGRGYNNR"
BASE COUNT          691 a          800 c          843 g          696 t
ORIGIN      
        1 ggtatatcct aaatagtgaa tagtgattgg tttatgctct actaatcact attcactcat
       61 tactcatcat ttcatgcccg aatcaccttt ccctcagaaa tccatcgcca tcatcggcgc
      121 gggtgcagcc ggactcgcgg ccgcatacga tctcgcgggc gcggggcaca ctgtcactgt
      181 ttacgaggct tcacccaacg tcggtggact ggcctccggt ttcaaagagc cgcaatggga
      241 ctggtcgatc gagcgcttct atcatcactg gttccaaact gattccagcg tgctcggctt
      301 gatcaacgag atcggcgcca gcgacaaagt tttattcccg cgacccatta cggcaatcta
      361 ctatcaggga aaattccatc ccttcgattc gatgttcacc aacatgccgc tttttctcct
      421 gcggcatttt ccgttgaagg atgtcatgcg cttcggtctg gccggggctt atctaaaatt
      481 cagttccaac tggaagtcgc tcgaacaagt aacggccgat gagtggatga ccaaacgcat
      541 gggccggaat gtttatgaat cgatgtggaa gccgctgctg gttggaaaat tttcggagta
      601 ctacgatcta gtcaacatgg cctggatgtg ggcgcgcatc cacgcgcgca cgacgcggct
      661 cggcactttc gtcggcggct ttcaagcctt cttcgacaca ctcgccgacg cagtgcgcca
      721 acgtggcgca tcgattcaac tctcaacggg cgtgcgcgag atcgtctcga acgaaaccgg
      781 cggcttgaaa ttattcacgc ccaacggcac gctcgattac gatgcctgcc tcgtcacgac
      841 ctcgccgcgc ttgatgagcc gcctcgcgcc gtccctgccg cccgattatt tatcgcagct
      901 tagcaaactc aaatcgatgg gcgcggtcgt attgatttta tcgctcaccc accaactgag
      961 tgaatcaggc atctactggc acaacttagt caaagaagcg ggcttccctt ttttagcgat
     1021 gtgtgagcac accaattttg tttcgcccga tcattttggc ggcgatcatc tgatctactg
     1081 cggcgattat ctttcgcccg atcacgaata cttcaaactc acggaaagtg agctgctcga
     1141 tcggtttgtg ccggctctct cgcgcttcaa tcccaagttc gatcgatcgt ggatcaagaa
     1201 agtatggctg ttccgtgagc catacgcgca gcctgtgccg ccgatcaatc attcgcagaa
     1261 catccctgat attcgcacac cgatcgatcg gttatacttc gcgtcgatgt cgcaagtcta
     1321 tccgtgggat cgcggcacga acttcgcggt cgagatcggt cggcggacgg cgaagatgat
     1381 cgtcagtgat tttgagcaga agcagaatac ttaaccgctg agcacgcaga gtgcgcggag
     1441 ttataaaatt atttctctgc gctttcagcg ctcgctgcgg aaaataaaat atcagcaaag
     1501 aggtaaagaa ttatgaaaag tattacagtt gtaggcgtcg gctatgtagg tctggtcacc
     1561 ggcacatgct tagccgatct agggcatcgc gtcgtgtgtc tggatgtaaa tgccgaacgc
     1621 gtcgagaatc tcaagaaggc catcctgccg atctatgagc cgggcctgga agagatcgtg
     1681 cggcgcaaca tgaatgccgg tcgtttgtcg ttcacgactt cgtatgccga agcgatcaat
     1741 ggcgcgagtg cggacgccgc ggccgaattt gtcttcatcg ccgtcggcac tccatcggcg
     1801 gtggatggca aagccgatct ggattacgtc cggcaagccg ccgaatcgat cgccgaagcg
     1861 atggatcatc cgttgatcgt cgtcaacaaa tcgaccgtgc ccgtcggcac cggcgactgg
     1921 accgcggaca tcattcgatc gaagcagaaa cgcgcggtga agttcaacgt cgtgtcgaat
     1981 ccggaatttt tacgcgaagg cagcgcgatc tatgatttca tgcagcctga tcggattgtg
     2041 cttggctcgc tcgagcgaga agcggccgaa aaagtcgcgc aactctattt gccgctgcgc
     2101 gctacgttga tgatcaccga tctgcgcacc gccgaaatga tcaagtatgc atcgaacgcc
     2161 ttcctcgcga cgaagatcag tttcatcaac gagatcgcca acatctgtga agaactcggc
     2221 gcggatgtaa aagaggtagc cacgggcatg ggcttcgata aacgcatcgg ccgcgcgttc
     2281 ctcgacgcgg gcgtcggata cggggggagt tgttttccga aagacgtatt agcgttggcg
     2341 cacatggcga aagaacgcgg acgtcatccg cagttactgc aagcggtcat ggacatcaac
     2401 gccgatcagc gcaatcacat cgtgtcgaaa gtgcgagaat tagccggtgg cagcgtgaag
     2461 ggcaagaaga ttggtgtgct tgggttagcg tttaaaccga acaccgatga tatgcgcgac
     2521 gccccggcga tcgatatcat caaggcgttg gagagtgaag gcgcaagcat actggcttat
     2581 gatcctgttg cgatgagtgt ggcgaagcag attatgccga acctcaagac cgtcgataat
     2641 ccgtacgacg cggcggcggg ctgcgatgca ttgatcttga tgaccgaatg gaacgaattc
     2701 aaacagttgg actttcataa gctgaaagcg gcgatgaaga gtccggccat gatcgatgcg
     2761 cggaatgtgt atgagccgga caagatggcg caggccggct ttaattatcg aggcgtagga
     2821 cgcggataca ataaccgctg acaggctgac aaggtgatgg ttgcagaccg ctcgtatgat
     2881 cattgatcgc gcgggcggtt tttgttttgt gcgttggggc gacgcattcg catatggagc
     2941 acgattgatt gtgctcctga ttgcggctgc gatgctactc gaccggaatc tttgaccgaa
     3001 tgcggcgccc ctacatcgat cgtgtataat
//
LOCUS       DASRTJ010000175         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_193522,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000175 DASRTJ010000000
VERSION     DASRTJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193522"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFF70_03705"
     CDS             <1..338
                     /locus_tag="VFF70_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43832.1"
                     /translation="PSTIEVDPQRIREVLENLIANALRYTPRDGSIRIKCEPVNQNFS
                     VSISDTGQGIAAEDLPHIFDRFYKGSDSRGTGLGLAIAKTLITAHGGEVFAESEVGQG
                     TTIRFTLPQ"
     gene            complement(430..>1782)
                     /locus_tag="VFF70_03710"
     CDS             complement(430..>1782)
                     /locus_tag="VFF70_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HZY43833.1"
                     /translation="RYGSPEGQEFAAQVMEFVRYHCMKTSVQLAKERGSFLAIEGSLY
                     DPKNLKWVPPSPIEPYTHDWDRPSIDWTEIVKGIQQYGIRNAAQTTVAPTGTIATVAG
                     CEAYGCEPVFALAYMRHMIDAEAGGNDGRTTLTYASPLFEQALIAAGIDAAGRQRIYD
                     DVMHSGTCQNVEGIPQTIRNAFVVSADITPDEHVRMQAAIQAFVDNSISKTCNFPESA
                     KEEDVAQAYLDAWTLGCKGLTVYVTGSRDKVVLETKATAQKKEQHVAIDAPLPATKRP
                     RPRKLHGETFRIGTPLGTAFITVNINGDDQPFEVFMNVGKAGSDTAAVAEALGRLISL
                     VLRVPSPMNPTERLQLVTEQLAGIGGGRSMGFGPNRVRSLPDGIAQALVEYLGGGSNG
                     NGNGHEHSANPIADPDSTVDLTQAPAIKIGDLCPECGEATYVNEEGCRKCYSCGHSEC
                     "
BASE COUNT          386 a          529 c          474 g          393 t
ORIGIN      
        1 ccccctcaac gatcgaagtc gatccccagc ggattcgtga agtcttagag aacctgatcg
       61 cgaatgcgct gcgctatacg ccgcgagatg gatcgattcg gatcaagtgc gagccagtga
      121 atcaaaactt cagtgtctcg atcagcgata caggtcaagg aattgccgca gaagacttgc
      181 cgcacatctt cgatcgattc tacaaaggca gtgattcgcg cggcacgggt ttggggttgg
      241 cgattgcgaa gactttgatc accgcacacg gcggagaagt ttttgcggag agcgaagtgg
      301 ggcaaggaac aacgattcga tttacgttgc ctcaataaaa agtaggggcg aggtaacctc
      361 gcccctacaa ttaaatccga aatatatctt cgtcgcggca gctgcgccca gcgtgccgcc
      421 gagattctat caacactcac tatgtccgca tgaataacat ttgcggcagc cttcttcatt
      481 cacatacgtt gcctcaccac attcaggaca gaggtcacca attttaatag caggcgcttg
      541 agtgagatcg accgtcgagt ccggatcagc gatcgggtta gcgctatgct catggccgtt
      601 gccattcccg ttcgatccgc cgcccagata ttccaccaaa gcttgagcaa tcccatccgg
      661 caacgatcga acgcgattcg ggccaaagcc catcgatcgg ccgccgccaa ttccggcaag
      721 ttgttcagtc acgagttgca atcgctcggt cggattcatc ggcgatggga cacgcagcac
      781 cagcgagatc aaccggccca acgcctcggc gactgctgcg gtatccgatc ccgccttgcc
      841 gacattcatg aacacttcaa acggttgatc gtcgccattg atattcacgg tgataaaggc
      901 cgtgcccagc ggcgtgccga tgcggaatgt ttcgccatgc agcttgcgcg gccgcggtct
      961 cttggtagcg ggtaaaggtg catcaatcgc aacatgctgc tctttctttt gcgcggtcgc
     1021 tttcgtttcc aaaacaactt tatcgcgcga accggtcaca tacaccgtga gacctttgca
     1081 gcccaacgtc caagcatcca gatacgcctg tgccacatct tcttctttcg ccgattcagg
     1141 aaagttgcac gtcttgctga tcgagttatc gacgaaggcc tgaatcgcgg cttgcatgcg
     1201 cacgtgctca tcgggcgtga tgtcggccga tacgacgaac gcattccgga tcgtttgcgg
     1261 gatgccttca acattttggc acgtgcccga gtgcataaca tcatcataga tacgttgtcg
     1321 gcccgctgca tcgatgcccg ccgcgatcaa cgcctgctcg aacaacggac tggcataggt
     1381 caacgtggtg cggccatcat tgccgccggc ttcggcatcg atcatgtgac gcatgtaagc
     1441 cagcgcgaac accggctcgc agccataggc ttcacagcct gccaccgtcg cgatcgtccc
     1501 tgtcggcgcg accgtcgtct gggccgcgtt gcggatgccg tactgctgaa tgcctttaac
     1561 gatctcagtc caatcgatcg aggggcgatc ccaatcgtgc gtgtacggct cgatcggact
     1621 gggcggcacc cacttgagat tctttggatc gtataagctg ccttcgatcg ccaggaacga
     1681 gccgcgctct ttcgccaact ggaccgaagt cttcatgcag tgatagcgca cgaactccat
     1741 cacttgcgcg gcgaactctt gaccttccgg gctgccatat cg
//
LOCUS       DASRTJ010000176         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000176 DASRTJ010000000
VERSION     DASRTJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFF70_03715"
     CDS             <1..532
                     /locus_tag="VFF70_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HZY43834.1"
                     /translation="NWKLERSRIGDSSLLNAKLLLPISRLSPLISKYNQRMKIYFSGA
                     ISAGRDNLPIYQYIVQRLQAQGHNVLSSHVADPEVLDREKDVPPRSVFDRDVAWVDEC
                     EVMIAEVSTPSTGVGYEYGYAIQIGKPVLCVYRSGLRMTKMILGNPASNLEVATYLTE
                     LELDQIIDAFLKSVTA"
     gene            672..>1751
                     /locus_tag="VFF70_03720"
     CDS             672..>1751
                     /locus_tag="VFF70_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZY43835.1"
                     /translation="MSDEPSDPGDHFFTQSAHAGDDADHPAGRPGSPPIYPAVTYTYD
                     DMQDLSRVLHGERSGYVYSRYGSPTVSTLEEAIASLENAEGAIAFSSGMAALHCAFIQ
                     CGITPDGPIIAAQDLYGATHALLAQIFAMYDKRSVHYVDMSDAAAVKFAIEHYAPRAV
                     LMEMISNPLLKVADLPQIAEWTHAAGATLIVDGTFASPYLIRALAHGADLVVHSGTKY
                     LGGHGDVMAGVIATDARRAGELRELIKIYGSNLGPFEAWILLRGLKTLALRMRQHCAN
                     ALQVARWLSDQVSISKVIYPGLPDHPQHSIAQRLFRENSFGGVVAFELRDAAEPEVFR
                     FMEALKLIRTGTSLGDVGSLLVYPAQ"
BASE COUNT          359 a          478 c          488 g          426 t
ORIGIN      
        1 aaattggaag ttggagagga gtcgcattgg cgactcttct ttactgaacg ccaagctcct
       61 ccttccaatc tcccgcctct cgccgctcat ttccaagtat aatcagcgca tgaaaatcta
      121 cttctccggt gcgatctcgg ctggccgcga caatctgcct atctatcagt acatcgttca
      181 gcgtttgcaa gcacaaggcc acaatgtttt atcgtcgcac gtggccgatc cggaagtgct
      241 cgatcgggag aaagatgtcc cgccgcgcag cgtgttcgat cgggacgtgg cctgggtcga
      301 tgaatgcgag gtgatgatcg cggaagtcag cacgccctcg accggtgtcg gctacgaata
      361 tggttatgcg atccagatcg gcaagccggt gttgtgcgtc taccgatcag gtttgcggat
      421 gaccaaaatg attctcggca atcccgcctc gaatttggaa gtcgccactt atttaactga
      481 attggaactt gatcagatca ttgatgcgtt cttgaaaagc gtgacagctt gagatattag
      541 atgtcaggct gagcgaagtc cctagagggc acagcctcta ggaatgtgcc acgaagcatc
      601 tcggctaatt gtgcgtgacc cttcgctacg ctccgggtga caatctttga aattcatctt
      661 gtccaacaat aatgtctgac gaaccgtctg atcccggcga tcacttcttt acgcaatctg
      721 cccacgcggg cgatgatgcc gatcatcccg caggtcgtcc tggctctccg ccgatttacc
      781 ctgccgttac ttacacttac gacgacatgc aagatttgtc ccgcgtgctg cacggtgaac
      841 gatcgggtta cgtgtacagc cgctacggca gtccgactgt ttctacgctt gaagaagcga
      901 tcgcttcatt agagaatgcc gaaggcgcca tcgcgttttc atcaggcatg gcggcgctgc
      961 actgtgcgtt cattcaatgt ggcatcacgc ccgatggacc gatcatcgcc gcgcaggatt
     1021 tgtacggcgc gacgcacgcg ctgctcgcgc aaatctttgc gatgtacgat aagcgctcgg
     1081 tgcactacgt cgatatgtcc gacgccgccg cagtgaaatt tgcgatcgaa cattatgcgc
     1141 cgcgtgcggt cttgatggag atgatctcca atccgttgtt aaaagtggcc gacctgccgc
     1201 agattgccga atggacgcac gccgctggcg caacgctgat cgtcgatggc acgtttgcct
     1261 cgccgtattt gattcgagcg ctggcgcatg gcgcggatct cgtcgtgcat tcaggcacaa
     1321 aatatttggg cgggcacggc gatgtgatgg ccggcgtcat cgccactgat gcgcgccgcg
     1381 cgggcgaact gcgtgagttg atcaaaatct atggctcgaa cctcggcccg ttcgaggcgt
     1441 ggattttatt gcgcggcttg aagacgttgg cactgcgcat gcggcagcat tgtgcgaatg
     1501 cgctgcaagt tgcgcgctgg ttatccgatc aagtttcgat ctcgaaagtg atttatcccg
     1561 gcttgcccga tcatcctcaa cactcgatcg cacagcgctt gttccgcgag aattcattcg
     1621 gcggcgtcgt cgcattcgaa ttgcgtgacg cggcagagcc tgaagtcttt cgcttcatgg
     1681 aggcgttgaa attaattcgc acgggaacaa gtctcggtga tgtgggcagt ttgctcgttt
     1741 atcctgcgca a
//
LOCUS       DASRTJ010000177         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_237106,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000177 DASRTJ010000000
VERSION     DASRTJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237106"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VFF70_03725"
     CDS             <1..1376
                     /locus_tag="VFF70_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43836.1"
                     /translation="ATIRIIPFANGQPICQPSNTVKFNIVAPAKPQTLPPPPLPLVAI
                     EASIVEFKPIQPANIAYQNCVVIIKNPYLDDPNYYFGTKGTPAEPWHYYPVGATLCPK
                     NYVAKSKSLLDYIEDSVNLVADIFSTLKDYAVTLTDQLNPLCIQAKLTSKAIDAGQKT
                     VADVCHAIAEATVDIGLAYVGLPPSLPNYDELQNASKGYVADLAVDQLGEDCPVRDVC
                     KKLIQQGMDFMIAKVKESQNGSQCFSDDAAQSLGYYPICEPKGAVTAPSPYGQIQPAI
                     VKVQISRQTDLSSSDFPDPSQYVNACSFVLNSGADNNSLIGQQIEIGINSKGQPVYWT
                     GEAISGELFKRVSAKIPTLAIGETLIVPFALTPSTTDYDSFRHPPYDTHNGFWLPGHL
                     ANLNYWATIDQTAVRGNDDWRYLYSGGSANLNLTGSCHTTVQGKPAGLSTFSAQYTLN
                     PLPSIGP"
     gene            1390..>2215
                     /locus_tag="VFF70_03730"
     CDS             1390..>2215
                     /locus_tag="VFF70_03730"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZY43837.1"
                     /translation="MMIKRLMIFLAAILIPMVLVACTGSNPQSTASIGTLAWIDAPLN
                     GSTLPLGPINIVSHSSDATGITQVELSVNGTVVKIDQNQDASQHLVVMNQMWTPTASG
                     NYQLFVRAQNSNGGWSNYAQAAITINGAPAPTQVPNVQQPTPTLFVPSPTPAQTGCVG
                     VPAIASFYSSTPAISAGDSVTLIWDAIINADSIEIDNGIGSVGPSGSRTVSPASTTTY
                     TLIARCGDQLATSQVPIRVTQPVPTASPTTSPTNPTIGAPTFSTNQFWYGLVGCGDM"
BASE COUNT          557 a          653 c          532 g          473 t
ORIGIN      
        1 gcgcgactat ccggatcatc cccttcgcca acggtcagcc gatttgccag ccgtcgaata
       61 cggtgaaatt caatatcgtg gctccggcca aaccgcaaac gctcccgcct ccaccccttc
      121 cgctcgttgc aatcgaggca agcattgtgg aatttaagcc gattcaaccg gcaaacattg
      181 cgtatcaaaa ctgcgtggtg attatcaaga acccgtatct ggatgatccg aattattact
      241 tcggcactaa aggcacaccg gctgaaccgt ggcactatta cccggtcggc gcaacactct
      301 gtccgaaaaa ttacgtagcg aagagcaaat cactcttgga ttatatcgag gacagcgtca
      361 atttagtggc tgatattttt agcaccctca aagattatgc ggtgaccctg acggatcaat
      421 tgaacccgct gtgcattcaa gcgaaactaa cctccaaggc gattgacgct ggacaaaaga
      481 ccgtcgctga tgtctgccac gccatcgccg aagccaccgt cgatatcggc ttggcctatg
      541 tcggtctgcc acccagcttg cccaactatg acgaattgca aaatgcaagc aaagggtacg
      601 tggccgatct ggccgtcgat caactgggcg aggattgtcc tgtcagggat gtctgcaaaa
      661 aactaatcca gcaaggcatg gacttcatga ttgccaaagt caaagaaagc cagaacggtt
      721 cgcaatgctt cagtgatgat gccgcccaat ctctcggcta ttatccaatt tgcgaaccga
      781 agggggccgt caccgcgccc tcgccttatg gtcaaattca gcccgccatc gtcaaggtgc
      841 aaatcagtcg ccagacagat ctttccagtt cggattttcc cgatccaagt cagtatgtca
      901 acgcttgcag cttcgttctt aactcaggcg cggacaataa ttcgttgatc ggtcaacaga
      961 ttgagatcgg gatcaattcc aaaggccagc cagtatattg gacaggtgaa gcgatcagcg
     1021 gcgagttgtt caaacgagta agcgcaaaaa ttcctacgtt ggctataggc gaaacactga
     1081 tcgttccgtt tgccttgacg cccagcacta cagattacga ctcattccgg catccgccgt
     1141 atgatactca taatgggttc tggctgcccg gccatctggc caatctcaac tattgggcaa
     1201 cgatcgatca aacggccgtg cgtggcaatg atgactggcg ctatctttat tcaggcggaa
     1261 gcgccaatct caatctaaca ggctcatgcc acacgacggt gcagggcaag ccggccggcc
     1321 tcagcacatt cagcgcacaa tacacgctca acccattgcc gagtataggc ccatagtcgg
     1381 gagactaaaa tgatgatcaa acgattaatg atctttctcg cagcgatctt gatcccgatg
     1441 gttctggtgg cctgcaccgg atcgaatccc caatcgaccg catcgatcgg cacactggcg
     1501 tggatcgatg caccgctcaa cggttcaacg ctgccgctcg gcccgatcaa catcgtctcc
     1561 cattccagcg atgcaacggg tatcactcaa gtagaattga gtgtaaacgg cacagtggtc
     1621 aagatcgatc agaatcaaga tgccagtcag catctcgtcg tgatgaatca aatgtggacg
     1681 ccgaccgcat caggcaacta ccaattattt gtgcgagcgc aaaattcaaa tggcggatgg
     1741 agcaattacg cgcaagccgc catcaccatc aacggcgcac cagcgcccac tcaagttccc
     1801 aatgtccaac aaccgacacc aactctattc gtcccatcgc ccacgcctgc acaaacgggt
     1861 tgtgtcggtg tgccggccat cgcttcgttc tactcatcaa cgccagcgat cagcgcgggc
     1921 gattcggtca cgctgatttg ggacgcgatc atcaacgccg actcgatcga aatcgataac
     1981 ggcatcggca gcgtaggccc atcgggttca cgcaccgtct cgcccgcctc aacaacgacg
     2041 tacacgttga ttgcacgctg tggcgatcaa ttggccacca gccaagttcc gattcgcgtc
     2101 actcagcccg tccccaccgc tagcccgacc accagcccga ctaatcccac gatcggcgcg
     2161 ccaacttttt cgacgaacca attttggtac ggactggttg gctgtggaga tatga
//
LOCUS       DASRTJ010000178         6370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_85426,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000178 DASRTJ010000000
VERSION     DASRTJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85426"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFF70_03735"
     CDS             complement(<1..595)
                     /locus_tag="VFF70_03735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130550.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HZY43838.1"
                     /translation="MVQFVLLRHRNTPNIHQLSVYGDYAALRKAVASMQPAEVIDVVK
                     ASGLRGRGGAGFPAGVKWSFIPKDRFPHYVTVNGDESEPGTFKDRELMEFNPHQVIEG
                     SAIAAYATGATRVYHYLRGEFKPIARVMQAAIDEAYAKNYLGENIFGSNVEIQMVVHL
                     GAGAYICGEESALLESLEGKMGQPRLRPPFPAIAGLYA"
     gene            complement(713..1144)
                     /locus_tag="VFF70_03740"
     CDS             complement(713..1144)
                     /locus_tag="VFF70_03740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43839.1"
                     /translation="MVNIRHLTVEDYNAAIALWQRSGLPSIRPQGRDSKDEFAQQIDR
                     GQIVIGLEDDGKLIGIVVATNDTRKGWINRLAIDPDDRRKGYGAQLIHAAEDALRSIG
                     IKVIAALIEDWNDASLALFKREGYAVHSDIVYVSKRDSESD"
     gene            complement(1156..1650)
                     /locus_tag="VFF70_03745"
     CDS             complement(1156..1650)
                     /locus_tag="VFF70_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZY43840.1"
                     /translation="MNKLLDKYHTEVDQILDKYPSDRKRSAVLPLLYIAQQEYGYCTP
                     EAINEVAEIAGVSSTEVESVVGFYTLFYDHPIGKHVVQICNDLPCALRGSDKFLDHAC
                     SKLKLDKHKVEHGGATTEDGSVTLESVVCLAACDKAPMMQVDLEYHERLTNEQFDQIV
                     DGLK"
     gene            complement(1763..2959)
                     /gene="nuoD"
                     /locus_tag="VFF70_03750"
     CDS             complement(1763..2959)
                     /gene="nuoD"
                     /locus_tag="VFF70_03750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZY43841.1"
                     /translation="MKGLVSQRALDGETVLLNMGPQHPSTHGVLRLLLELDGETVISC
                     IPDVGYLHTGIEKSIEDKTYTKAIPLTDRMDYLSPMSNNLGYVLAVEKLMDVDVPLRA
                     QYIRVILCELQRISSHLVWLGTHAIDLAAMSVFLYCFREREKILDIFEMVSGQRMMVS
                     YFRVGGLWRDLPAGFEDAVRAFLDHFPSEIDEYEGLLKNNPLWLDRTKNIGVVSAKDA
                     IAWGLTGPSLRGSGVNWDIRKAQPYSAYEHFDFDIPVANEGDVYARFWCRVQEMRQSL
                     RIIDQGLKKLPKGAFITSNRKVAPPPKEELAYSMESLIHHFKLWTEGFTPPVGEVYVS
                     VESPRGEFGCYIYSDGSPKPCRVHFRAPSFANLSGLHLMTHGVFIADLVAIIGSIDIV
                     LGDVDR"
     gene            complement(3211..3720)
                     /locus_tag="VFF70_03755"
     CDS             complement(3211..3720)
                     /locus_tag="VFF70_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZY43842.1"
                     /translation="MTDPIIELVKTKFSDSIEDTLEFRGETTFVVKADRLIDVCTFLR
                     DDQHFDFLSDVSGVDHYPAEPRFDMNYHLYSMENNRRLRLKVRLAGADPIVPSVTSVW
                     PGANWHEREVWDLIGIKFSGHPDLRRIFMPDDYLGHPLRRDAPLVYEEPQFTHNFNDV
                     GKRKPYAKE"
     gene            complement(3867..4370)
                     /locus_tag="VFF70_03760"
     CDS             complement(3867..4370)
                     /locus_tag="VFF70_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZY43843.1"
                     /translation="MGLESKLGNMGVVTARLEDLINWGRTNAMWPLGFGLACCAIEMM
                     SAQASHYDMSRFGMELMRASPRQSDLMIVAGRVSRKMAPVLRRLYDQMPDPKWVLSMG
                     DCASCGGVYNNYAIVQGVDEIVPVDVYVAGCPPRPEALIDGIVKLHEKIRRSHPLKEA
                     RGEPIKV"
     gene            complement(4361..4717)
                     /gene="ndhC"
                     /locus_tag="VFF70_03765"
     CDS             complement(4361..4717)
                     /gene="ndhC"
                     /locus_tag="VFF70_03765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZY43844.1"
                     /translation="MLEAYIPIAILFIVATAISILLIVIGVVFGPRSKSLRKSSAYES
                     GMMPIGPGTRRMTVRFYLIAVLFILFDVEIIFFYPWAVNFRALGLFGFVEMLVFVGIL
                     LVGYIYVWKKGALEWD"
     gene            complement(4937..>6370)
                     /locus_tag="VFF70_03770"
     CDS             complement(4937..>6370)
                     /locus_tag="VFF70_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HZY43845.1"
                     /translation="FVMTPEGLIAKHGDEIRPGDWLLTPRAIPDTQIQSQVDLLEWIS
                     PEFIDHRQRVNKTLQWDDQAIWSRRDQTLNRYAANSQELAEFLGIAVAEASGRDTLSL
                     AVGSDRELAGRIIDLSNRLFGASRVSLAEVSAEAMSRYVANAPGISALKPKEQFRPVV
                     GGRLLAHVIGNMIGHGAVNKHIPAYIFNASQSVQQVFLKSLISGDGHVRVRPIKSQAE
                     IGITTVSPQLVADVILLCRQLGIWARVEKHGQAGLRRGMQHQLSYRVAISGQANIARL
                     LDGINVAHSTHPGTFDGIPRELIGLKRTPSQKRLKRTTSDEYFPCGVPVIPKKSRAQY
                     ARMMHWLQDWAVVEVNSVENVSPKSPYVYDLVVPNNHSFVAGSGLVLVHNSGGEQFRV
                     NFAVRIALSKLLARRAGAQLQTLVIDEGFGALDSTGRDKLVEAINAIQDDFQRILVIT
                     HLDELKDAFPVRIDVTKTENGSQIAIV"
BASE COUNT         1514 a         1827 c         1588 g         1441 t
ORIGIN      
        1 gcgcatacag accggcgatc gccgggaacg gcgggcgaag gcgcggctga cccatcttac
       61 cttccaatga ttccaacagc gccgattctt caccgcagat gtacgcgcct gcgccgaggt
      121 gcacgaccat ttgaatctca acgttcgagc caaaaatatt ctcgcccaaa taatttttgg
      181 cgtacgcttc atcgatcgca gcctgcatca cacgcgcgat cggcttgaac tcgccgcgca
      241 aataatgata gacgcgcgtc gccccggtcg catacgccgc aatcgccgat ccttcgatga
      301 cttgatgcgg attgaactcc atcaactcgc gatctttgaa cgtgcccggc tcggactcat
      361 cgccattgac tgtcacgtaa tgcgggaatc gatctttggg aatgaacgac cacttcacgc
      421 cagcgggaaa gcctgcgccg ccgcgcccgc gcaggcctga agccttgacg acatcgatca
      481 cttctgcggg ctgcatcgac gcgaccgcct tgcgcagcgc ggcgtaatcg ccgtagacgg
      541 agagttgatg aatattgggt gtgttgcgat gacgcagtaa aacaaattga accatcaacg
      601 aatcctcatt gaactaaacc cacaggattc gtcattgcga ggagcgcagc gacgaagcaa
      661 tctcattcat cgatcgggag attgcttcgc tctgctcgca atgacttcga acctaatcac
      721 tttccgaatc tctcttgctc acgtagacaa tatctgaatg aaccgcatac ccttcgcgct
      781 tgaacaaggc cagcgacgcg tcattccaat cttcgatcaa cgccgcaatc actttaatgc
      841 cgatcgatcg cagcgcatct tccgccgcgt gaatcaactg cgcgccataa cccttgcgcc
      901 ggtcatccgg atcgattgcc aggcgattga tccagccctt acgtgtgtcg ttcgtcgcga
      961 cgacgatgcc gatcaatttc ccgtcgtctt ccaagccgat cacgatctgt ccacgatcga
     1021 tctgctgggc gaattcgtct ttgctatcac ggccctgtgg ccgtatgctc ggcaaacccg
     1081 atcgctgcca caacgcgatc gcggcattgt aatcttcaac tgttaaatgt cgaatgttaa
     1141 ccacggatta cttggctact tcaacccatc gacaatctga tcgaattgtt cgttcgtcaa
     1201 gcgttcgtga tattccagat cgacctgcat catcggcgct ttgtcacagg ccgctaagca
     1261 caccacggac tccaaagtga ccgagccatc ttccgtcgtt gcaccaccat gctcgacttt
     1321 atgcttgtcc agttttaact tagagcaagc gtgatcaaga aacttgtccg agccgcgcag
     1381 cgcgcacggt aagtcattgc agatctgaac aacgtgcttg ccgatcggat gatcatagaa
     1441 caatgtatag aagcccacga ccgattcgac ttcggtcgaa ctcacgcctg cgatctcggc
     1501 gacttcgttg atcgcttcgg gcgtgcagta gccgtattcc tgctgcgcga tatacaacaa
     1561 cggcagcacg gccgatcgct ttcgatcgct gggatattta tcgagaatct gatcaacttc
     1621 agtgtggtac ttatcgagta gtttgttcat tatcactcag gtcaaacttt taattgtcat
     1681 ttcgagcgaa gcgagaaatc tttcctgaca atcacagaga tttctcgtcg cgcgttgcgc
     1741 tgctcgaaat gacactgcgc atttatcgat ccacatcgcc caacacaata tcgatcgagc
     1801 cgataatcgc aaccaagtcg gcgatgaaga cgccatgagt catcaagtgc aggccggata
     1861 gattcgcaaa cgacggcgcg cggaagtgca cgcggcacgg cttgggcgat ccatcgctat
     1921 aaatgtagca gccgaattcg ccgcgcggcg attcgacgct gacgtacact tcgccgacgg
     1981 gcggcgtaaa gccttccgtc cacaacttga agtgatggat cagcgactcc atcgagtaag
     2041 ccaattcttc tttgggcggc ggcgcaactt tgcgattgct ggtgatgaat gcgcccttcg
     2101 gcagtttctt caacccttga tcgatgatgc gtagcgattg gcgcatttcc tgcacacggc
     2161 accagaagcg cgcatacaca tcgccttcat tcgcaactgg aatatcaaaa tcgaaatgtt
     2221 catacgctga atacggctgc gctttgcgga tgtcccaatt gacgcccgat ccacgcaagc
     2281 tcggcccggt cagtccccac gcaatagcat ccttggctga aacgacgccg atgttcttcg
     2341 tgcgatcgag ccacaacgga ttgttcttca gcagcccttc gtactcatcg atctcggacg
     2401 ggaagtgatc gaggaaggcg cggaccgcat cttcaaagcc cgccgggaga tcacgccata
     2461 ggccgccgac gcggaagtat gagaccatca tgcgttggcc ggacaccatc tcgaagatat
     2521 ccagaatttt ttcgcgctcg cggaagcagt acaggaagac gctcattgcc gccagatcga
     2581 tcgcatgcgt gccgagccag acgaggtgac tgctgatgcg ctgcagttcg cacaagatca
     2641 cgcggatgta ttgcgcgcgc agcggcacat cgacgtccat caatttctcg acggccagca
     2701 cgtaaccgag gttgttgctc atcggcgaga ggtaatccat gcgatcggtc aacgggatcg
     2761 ctttggtgta cgtcttgtcc tcgatcgatt tttcgatgcc ggtgtgcaga tagccgacat
     2821 cgggaatgca ggagatcacc gtctcaccat cgagttcgag gagcaagcgc aagacgccgt
     2881 gcgtcgaggg gtgctgcggg cccatgttta gcaggacggt ctcgccgtcg agcgcgcgct
     2941 gtgagacgag acctttcatc tctttgatgt cgtcgacggt caattccatt aattgatatt
     3001 cattagcagc cattgtgttg ctccaccact accaataaga accactgaca cttgcaccgc
     3061 gctgcgcgcg gtagcgcgca agtgcaggtg ttacacagat tacactgatt ttttcttctt
     3121 caattgtcat cctgagtgca acgaaggatc tagcacaatt agccgagatt cttcgccgcg
     3181 taaacgcggc tcagaatgac acgctgataa tcattctttc gcgtacggct tgcgcttgcc
     3241 gacatcatta aagttatgcg tgaactgcgg ctcttcataa accaatggcg catcacggcg
     3301 cagcggatgg ccgagataat cgtcgggcat gaaaatacgg cgcagatcag gatggccgct
     3361 gaatttgatg ccgatcaaat cccagacttc gcgttcatgc caattcgcac cgggccacac
     3421 actcgtcacc gatggaacga tcgggtcagc acccgccaat cggactttca aacgcagccg
     3481 ccgattgttc tccatcgaat acagatgata gttcatatcg aaacgcggtt cggccggata
     3541 atgatcgacg ccgctgacgt ccgataagaa atcgaaatgt tgatcgtcgc gcaggaacgt
     3601 gcagacatcg atcaaccgat cggctttcac gacaaacgtg gtttcgccgc gaaactcgag
     3661 cgtgtcttcg atcgaatcag agaatttggt tttgacgagt tcgataatcg gatcagtcat
     3721 attggttctc acacttcact ttgctcaagc cgccgtctcg gcggcgttta tgctcgcgcc
     3781 gaggacggcg cggagagcaa catgaatcac gcacaaaaaa tggatcggct gacggaatcc
     3841 ccgccgccga tccatcgatc gttcagttaa actttaatcg gttcgccccg tgcttcttta
     3901 agcggatgac tgcgccgaat tttttcatgc agcttgacga tgccatcgat caatgcttcc
     3961 gggcgcggcg gacaaccggc cacgtacaca tcgaccggca cgatctcgtc gacgccctgc
     4021 acgatggcgt agttgttgta cacaccgccg catgaagcgc aatcgcccat cgacagcacc
     4081 catttcggat cgggcatttg atcgtacaag cggcgcagca ccggcgccat cttgcggctg
     4141 acgcggcccg cgacgatcat caaatcgctt tgacgtggac tggcgcgcat caactccatg
     4201 ccgaagcgcg acatgtcata gtggctggcc tgcgcggaca tcatttcgat cgcgcagcag
     4261 gctaaaccaa aaccgagcgg ccacatggca ttcgtgcgac cccagttgat caagtcttcc
     4321 agtcgagctg tgaccacgcc catgttgccg agtttacttt ctaatcccat tccagcgctc
     4381 cctttttcca cacatagatg tagcccacta aaagaatccc gacgaagacc agcatttcga
     4441 cgaagccgaa caagccgagc gcgcggaagt ttaccgccca cggatagaag aaaatgattt
     4501 cgacatcgaa caggatgaac agcacggcga tcaaatagaa gcggacggtc atgcggcggg
     4561 tacccggccc gatcggcatc atgcccgatt cgtacgccga cgatttgcgc agcgacttgc
     4621 tgcgcggtcc gaagacgacg ccaatcacaa tgagcaaaat actaatagcg gtggcaacga
     4681 taaacaagat ggcgatgggg atatacgctt cgagcatctc aactccctaa gcccgcttga
     4741 agatcagcac ggcgtcgagg aaaatttaga agaactttgt agattctagc acaagcacta
     4801 gggagtgtca aacaaaatca atgaacaatg tgcaatgatc gattaacaat gagcacgaac
     4861 tatcgagttc aaagcctccg ggcgctttgc gttctctgcg gtaaatttct tcttcgtgcc
     4921 ccttcgtgga tcaatcctac acaatcgcga tctgcgatcc attctccgtc ttcgtcacat
     4981 cgatccgcac cgggaacgca tctttcaatt cgtcgagatg cgtgatcacc agaatccgct
     5041 gaaagtcgtc ttgaatcgcg ttgatcgctt cgaccaattt atcgcggccc gttgaatcaa
     5101 gcgcaccgaa accttcatcg atcacgagtg tttgcagctg cgcacccgct cgccgcgcca
     5161 gcagtttgct caacgcgatg cgcactgcaa agttcacgcg gaactgttcg ccgccggaat
     5221 tgtgcaccag aactagccca ctgccggcta caaatgaatg attgtttggc acaactagat
     5281 catacacata gggagatttg ggcgaaacat tttcgaccga attaacttcc acgacggccc
     5341 agtcctgcaa ccagtgcatc attctggcat actgcgcacg gcttttcttt ggaataaccg
     5401 gcacaccaca cgggaaatat tcgtcgctgg tagtacgctt cagtcgtttt tgacttggcg
     5461 tgcgtttaag cccgatcaac tccctgggta ttccatcaaa agttccagga tgcgtggaat
     5521 gagcaacgtt gattccatct agcagacgcg cgatatttgc ctgaccggaa atcgctacac
     5581 gatatgatag ctggtgctgc attccacgtc gtaggccggc ttggccgtgt ttttctacac
     5641 gcgcccagat acccaattgt cggcagagca atattacatc ggccactagc tgaggactaa
     5701 ctgtcgtaat gccgatctcg gcctggcttt ttattggtcg gacccgcaca tgaccatcgc
     5761 cgcttatcaa agatttcaaa aatacttgtt gcacgctctg agacgcatta aaaatgtaag
     5821 ctgggatatg tttgttaacc gcgccgtgac cgatcatatt gcctatgaca tgcgccagca
     5881 agcgaccgcc gaccactggc cggaattgtt ccttcggctt gagcgctgaa atccccggcg
     5941 cgttagcaac gtagcgggac atcgcttcag ccgaaacctc agctaatgaa actcgtgatg
     6001 cgccaaatag ccgattcgac aaatcgatga tgcgtccagc caattcacga tcacttccaa
     6061 ccgctaacga taacgtgtcg cgtccggacg cttcggcgac tgcgattcct agaaattctg
     6121 ccagctcctg agaattagcc gcatagcgat tcagcgtttg atcgcggcgt gaccatatcg
     6181 cttgatcatc ccactgcagc gttttattga cacgctgtcg atgatcgata aactctgggc
     6241 taatccattc gagcaaatcg acttgcgatt gaatctgtgt atcaggaatg gcgcggggtg
     6301 tcagcaacca atcaccagga cgaatttcat caccgtgctt ggctatcaat ccctctggcg
     6361 tcattacaaa
//
LOCUS       DASRTJ010000179         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269350,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000179 DASRTJ010000000
VERSION     DASRTJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269350"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFF70_03775"
     CDS             <1..1037
                     /locus_tag="VFF70_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZY43846.1"
                     /translation="SPFGVNTFLEQEVEPAKRDRQLQMIQDAGFKWIRQQFPWADIEI
                     SAKGNFDDCRNPPCISAWTKYDNIVELAAEHQINIVARLDAPPNWARSTPGDFAPPAN
                     LNDYADYVAAVATRYKGKIKFYQIWNEPNNYPEWGEQPVDPEAFTQLLCMAYQRIKQI
                     DPEANVIAPALTPTISLDPGPGPNTGLNEFIFLQRMYDANAGQCFDIMSAQGYGLFTG
                     PTDRRLRPRVVNFSRPMYIRDIMVKNGDAHKPIWISEMNWNSVPDSIANKRFGQVTLD
                     HQARYLPIAYDRIQTEYPWVGVAFTWYFKPATNAEKDQSKYYFRLVDPDFTPLPVYDA
                     IKTYANQFNH"
     gene            1221..>2882
                     /locus_tag="VFF70_03780"
     CDS             1221..>2882
                     /locus_tag="VFF70_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY43847.1"
                     /translation="MRLSRSTIILLIILIFAAALRFYRIDAQSFWNDEGNSARIAERS
                     LQLITEGAAGDIHPPLYYYVLHFWRGAFGSSELALRSLSAILGIALVWLTYLIAKRLF
                     DVKIGLIAVLVAAIDPFQIYYSQEARMYMLLAVIGAASTLVLIQLLERWGSAYQPISQ
                     SVNQGAQQKITSSPFHLFILSLLYALGLYTHYAFPFVIVTHFLIVIAWALTDRRRLLR
                     HVLIWIGIGAAAGVLFSPWLPIAIRQITTWPSTRVAIDTGAMLLDTLRLYVLGPTFPT
                     ADATIALLIAGFLVIVSLWNPDSFDEPQVDATHPLPHALRSGSIVLWWLIPIALIFGF
                     GLFKESYLKFLLIGSPAFCILLGRGILTGFGIARNALQMPKELAGTPLLSFGWSIVMI
                     FLVVLIALPTTTALANYYFDPKYARDDYRGIAQTITSLQRDGDAVILDSANQWEVFTY
                     YYPDGPNVFPLPKQRPIDQAAVEAQLSDIAAKYKRLFVLYWGDSEADPQRVIESWLDG
                     HTYKAPELLYGSVRFVAYAVPSILSDKPTQLSGARFGDHIILNGYT"
BASE COUNT          623 a          798 c          753 g          708 t
ORIGIN      
        1 tgagtccgtt cggggtgaat acttttctgg aacaggaagt cgaaccggcc aaacgtgatc
       61 ggcaacttca aatgattcaa gacgccgggt tcaaatggat ccgccagcaa tttccgtggg
      121 ctgatattga aatctcggcg aagggcaact ttgatgactg tcgcaatcca ccttgcatca
      181 gtgcgtggac gaagtacgat aacatcgtcg aactcgccgc ggaacatcag atcaatatcg
      241 ttgcgcgatt agatgctccg ccgaactggg cacgatcgac accgggcgat tttgcgccgc
      301 cggcgaatct gaatgactat gctgattacg tggccgcagt ggcgactcgc tacaaaggta
      361 agatcaagtt ctatcaaatt tggaacgagc cgaacaatta tccggagtgg ggcgagcaac
      421 cggtcgatcc ggaagcattc actcagttgt tgtgcatggc ctatcagcgc atcaagcaga
      481 tcgatccgga agcgaacgtc atcgcacccg cgttgacgcc gaccatctcg ctcgatccgg
      541 ggccgggacc aaacaccgga ctcaatgaat ttattttctt acagcggatg tatgatgcga
      601 acgcaggcca gtgtttcgac atcatgtcgg cgcagggcta tggcttattt accggcccaa
      661 ctgatcgacg cttgcgacca cgcgtcgtca acttttcgcg gccaatgtat atccgcgaca
      721 tcatggtgaa gaacggtgac gctcacaaac cgatctggat cagcgagatg aactggaact
      781 ccgtgccaga ttcgatcgcc aacaagcgct tcggccaagt cacgctcgat caccaggcac
      841 gctatttgcc catagcgtat gatcggattc agacagaata tccgtgggtc ggcgtcgcgt
      901 tcacgtggta cttcaagccc gcgaccaatg cggaaaagga tcagtccaag tattacttcc
      961 gcttagtcga tccggatttc acgccgctgc cggtgtatga tgcgatcaag acttacgcaa
     1021 atcaatttaa ccactagtta cacggatttc actgattagg cctcttcagc aaggcatgca
     1081 taacgtcatt cccgcgatgc cgggtatcca gagcgaaata actggacacc cgcctccgcg
     1141 ggtgtgacag cgcgtaaatg agtgattaaa aatcaaaaac taaatctgcg taatcagtgt
     1201 tatccgtggt ttcaattcat atgcgacttt ctcgctcaac gatcattctc ttaattatcc
     1261 ttatctttgc cgctgcccta cgcttctacc gcatcgatgc gcagtcgttc tggaacgatg
     1321 aaggcaacag cgcgcggatc gcggaacgat cgctgcaact gatcaccgaa ggtgcggcgg
     1381 gcgatattca tccgccgttg tattactatg ttcttcactt ctggcgcggc gcatttggat
     1441 cgagcgagct agcgttgcga tcgctctccg cgattctggg catcgcgttg gtgtggctca
     1501 cctacttgat cgccaaacgc ttattcgatg tgaagatcgg tttgattgcc gtcctcgtcg
     1561 ccgcgatcga tccgtttcag atttactact cgcaagaagc gcgcatgtat atgctgctgg
     1621 ctgtcatcgg cgcggcctcg acactggtgc tgattcagtt gcttgaaaga tggggatcag
     1681 cttatcagcc aatcagtcaa tcagttaatc agggcgctca acaaaagatc acctcgtcac
     1741 cttttcacct tttcatcttg tcattgcttt acgcgcttgg tctctacacc cactacgctt
     1801 tccccttcgt catcgtcact cacttcttga tcgtgatcgc ctgggcttta accgaccgaa
     1861 gacgcttact gcgacatgtc ctcatctgga tcggaattgg cgcggcggcg ggcgtgctct
     1921 tcagcccgtg gctgccgatc gcgattcgac agatcaccac gtggccttcc acccgtgttg
     1981 cgatcgatac cggcgcgatg ctgctcgaca ccctgcgatt gtatgtgctt ggcccgacgt
     2041 ttccgacagc cgatgcgacc atcgcgttgc tgatcgccgg cttccttgtg atcgtgagtc
     2101 tgtggaatcc cgattcgttc gatgagccgc aggtcgatgc gacacatccg ctgccgcacg
     2161 cgctgcgatc cggatcgatc gtgttgtggt ggctgatccc gatcgctttg atcttcggtt
     2221 tcggactgtt caaagaatcg tatctcaagt ttttgctgat cggctcgccc gcattttgta
     2281 tcttgttggg acgcggcatc ttaactggat tcggcatcgc gcggaacgct ttacaaatgc
     2341 cgaaggagct ggcgggcacg ccgctgctta gcttcgggtg gtcgatcgtg atgatcttcc
     2401 tcgttgtgct gatcgcgctg ccgacgacga ctgctttggc taattactac ttcgatccga
     2461 aatatgctcg cgatgattat cgcggcatcg cacaaaccat cactagtctg caacgcgacg
     2521 gcgatgcggt cattctcgat tcggccaatc aatgggaagt gttcacgtat tactatcccg
     2581 atggcccgaa tgtctttccg ctcccgaagc agcgcccgat cgatcaagcg gcggttgaag
     2641 cgcagttaag cgacatcgcc gccaaataca agcggctgtt tgtgctgtat tggggcgaca
     2701 gcgaagccga tccacagcgc gtgatcgaat cgtggctcga tgggcatact tacaaagcac
     2761 cggaactgtt gtatggatcg gtgcgcttcg tggcgtacgc cgtgccgagt atattatctg
     2821 acaagcctac tcaactcagc ggtgctcgct tcggcgatca cattatcctc aacggatata
     2881 ca
//
LOCUS       DASRTJ010000180         5532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117826,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000180 DASRTJ010000000
VERSION     DASRTJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117826"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFF70_03785"
     CDS             <1..595
                     /locus_tag="VFF70_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43848.1"
                     /translation="SIDLQTTSASPVAPSPTPSTQAGHGKDVFWLNCMPCHGDQGQGL
                     TDEFRVRQYPTEDQNCWKSGCHGDRPYDNGFKLPKTIPALIGPGTLSRFSTAQDLFVF
                     ISKAMPFNKPGSLSQTEYLQVMAYMLSSNQLVAPDSQIDAVSLASITVHALATDATPA
                     PNSASSTSAELPPAFWGIIIFLIIATILSFVTRRRRK"
     gene            630..2720
                     /locus_tag="VFF70_03790"
     CDS             630..2720
                     /locus_tag="VFF70_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY43849.1"
                     /translation="MSAHPATILVVDDDKKISDFIIDVLSEGGHQARPAYGGQEALDR
                     LATRERDEIDLVLLDVMMPDIDGFTVARRLRANPATEYLPIIMVTSRDRSSDAAQGLE
                     LGADDYITKPFDPRELLARITAMLRLRRAEQNLIGRNRALAALNTVAQTIGRAVELPD
                     VLRSALDQVLASLDLIGGMITLKASTGKQTPAVQRWPAFDMQPNSKISDRVAENGHSE
                     KFYLRARQANQLQAACVPLRSRNDVLGTLVVAGSNVHADDALDLLMTIGSQIGAAVER
                     ARLYEEAQHRSEDLAVLNEITRAVTSSLDLDQVLKTSLHGIRQILHVQAGSLILVEPE
                     SGEWQFRNTLNTLEAYLRADALQPHTGIVHRVIDSREPLILNEVPLESKLHGGGSLDA
                     SRSTTLVLRNILAVPMIVKSRAVGAIVVINKLEGAFTSDDLEMLQFLAAGVAIAAENA
                     RLYGELADFAKELERSQAQLVQAEKLAATGRLAASIAHEINNPLQAIHNCLHLAINRP
                     LTEAKKQHYLVMAQEEVERLITLVQRTLEFYRPSKGRSALTRVNDLIENVLALANKQL
                     EQVSINVHKNLANDLPEISAVPDQLVQVWLNLIINAIEAMPDGGELTITTSATDEWLQ
                     VAIRDTGSGVDPEDAAKIFEPFYTTKATGTGLGLSVSYGIIQRHGGRIDVNGVPGEGT
                     AFTVSLPIGVIDDL"
     gene            2815..3615
                     /locus_tag="VFF70_03795"
     CDS             2815..3615
                     /locus_tag="VFF70_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY43850.1"
                     /translation="MNSLINSIPHLLIVDDERATRESLEEILTLEGFQVALADSGEQA
                     LAVLRETPIDLMLLDLKMKGMDGLQTTETAKQIAADTVIIMLTAHGTLESAVAAMRRG
                     AFDYLLKPATVSDIIASVQRGLSHRAQMLRQRDLVGLMQRAITELQTPVTQAEAASQP
                     DRYLRARGITLDLQRHVASAQDHVLDLTLTEFKMLAYLMDHPDRVISPRELVAAVQGY
                     EADEHEARAIVRVHIRRLRQKIEPDPDQPQHVLNVRGVGYLFAASDTG"
     gene            3800..4651
                     /locus_tag="VFF70_03800"
     CDS             3800..4651
                     /locus_tag="VFF70_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43851.1"
                     /translation="MTAKNILIVDDEPKVGYTLKESLESLGSEYVVAQAYSAEDALSV
                     LARKPYDLVVTDLRMPGMNGLDLIGRVKRDAPQTQTILITAYGSNEIAEASDRLQAAH
                     YFTKPFRIDEFVRVVVDTLKTPASKGASPAANKHFKWLVQRLNDLRFEVGAQYVILAN
                     MSGRLMAESGTIDGFDPALLASMLSRSLSPSTEIAQQLRGERVFNLVYHEGARYDVYA
                     ANVEEDLFMLLAFDRRQGVSRIGMVWLYIKRAIQELQTRIESQGNPPEVGGRLLPGAN
                     ERGYFLR"
     gene            complement(4797..>5532)
                     /locus_tag="VFF70_03805"
     CDS             complement(4797..>5532)
                     /locus_tag="VFF70_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY43852.1"
                     /translation="IRDAGITRISMTTNGLRLTELAKPLKEAGLERVNVSVDTLNPEK
                     FHRITRWGHLDQVLEGVHAADAHGLTPLKINAVVARGFNEDDVADLAALTLTHRWQVR
                     YIEMMPFGDVAAFAQDATVSQAEIMQRIEDRLGRLEPIEDAKLDGEARLYKLKGALAP
                     IGFISTLTNPFCASCTRVRLTADGKLRLCLLRDDEGDLLTPMRKGASDEEMEKLIRSL
                     VFRKPWGHDLADGVIPLQRVMSQIGG"
BASE COUNT         1239 a         1496 c         1528 g         1269 t
ORIGIN      
        1 ctcgatcgat ctgcaaacca cttccgcctc tcccgtcgct ccatcaccca caccgtccac
       61 gcaggctgga catggcaaag atgtattctg gttgaactgc atgccgtgtc acggcgatca
      121 aggtcagggt ttgacggatg agtttcgcgt gcggcaatat ccgactgaag atcaaaattg
      181 ttggaagtct ggttgtcatg gcgatcgacc gtatgacaac ggctttaagt tgcctaaaac
      241 gatccccgcg ctgatcggtc cgggcacact gtcacgtttt agcaccgcgc aggatttgtt
      301 tgtctttatc agcaaagcga tgccgttcaa caaacccggc tcgttaagcc aaacggaata
      361 cttgcaagtg atggcctaca tgttatccag caatcaactc gttgcgcctg attcgcagat
      421 cgatgcggtc tcactggcga gcattactgt gcacgcactg gcaaccgatg caacccctgc
      481 accgaattcc gcttcatcaa cctccgcaga attgccgcct gcgttttggg gcatcatcat
      541 cttcttgatc atcgccacga tcttgtcgtt cgtcacgcgc cgacggcgca agtaaaatcc
      601 gaaatcccaa atggccttat aatcacagca tgtctgccca tcccgcgact atcctcgtcg
      661 tcgacgacga caaaaaaatc tccgacttta tcatcgatgt cttaagcgaa ggcggtcatc
      721 aagcccgtcc cgcttacggc ggacaagagg cgctggatcg attagcgacg cgcgagcgtg
      781 acgagatcga tctggtgctg ctcgatgtga tgatgccgga catcgatggc tttaccgtcg
      841 ctcgccggtt gagggccaac ccggccaccg agtatctgcc gatcatcatg gtcaccagcc
      901 gcgatcgatc gagcgatgcg gcgcaaggtt tggaacttgg tgcggacgat tacattacca
      961 agccattcga tccgcgcgag ctgctggcgc gcatcacggc catgctgcgt ttgcgccgcg
     1021 ccgagcagaa tttaatcggc cgcaatcgag ccttagcggc gctcaatacg gtcgcccaaa
     1081 cgatcgggcg agccgtggaa ctgccggatg tgctgcgatc ggccttggat caagtgctgg
     1141 cctcgctcga tctgatcggc ggcatgatca cgctcaaagc atcgacgggc aagcaaacgc
     1201 ccgcggtaca gcgttggccg gcgtttgata tgcagcccaa tagcaaaatc agcgatcgcg
     1261 tggccgagaa tgggcattcc gaaaaatttt atttgcgcgc gcgtcaagcg aatcaactgc
     1321 aagccgcgtg tgtgccgctg cgcagccgca atgatgtctt aggaacatta gtggtagccg
     1381 gatcgaatgt tcacgccgat gacgcgctgg atttgctgat gacgatcggc agccagattg
     1441 gcgcagcagt ggagcgcgcg cgcttgtatg aagaagcgca gcatcgcagc gaagatttgg
     1501 cggtgctgaa tgagatcacg cgcgcagtca cctcgtcgct cgatctcgat caggtgttga
     1561 agacttcgct gcacggcatc cggcagattt tacacgtgca agcaggctcg ttaattttgg
     1621 tcgaaccgga gtcgggcgaa tggcagtttc gcaatacact caatactttg gaagcctatc
     1681 tgcgcgccga tgcgctgcag ccgcataccg gtattgtcca tcgcgtgatc gattcgcgcg
     1741 agccgctgat tcttaacgaa gtcccgcttg aatcaaaatt gcatggcggt ggatcgctag
     1801 atgccagccg atcgacgaca ctggtgctgc gcaacatcct ggccgtgccg atgattgtca
     1861 agagccgcgc ggtcggcgcg attgtcgtca tcaacaaact cgaaggcgcc ttcacgagcg
     1921 atgatttgga aatgctgcaa tttctggcgg cgggcgtggc cattgccgcg gagaatgcgc
     1981 gcttgtacgg cgagttggct gattttgcta aagagttgga gcgatcgcaa gctcaattag
     2041 tgcaagccga aaaattagcc gctactggcc gcttggccgc ctcgatcgcg cacgagatca
     2101 ataatccctt gcaggcaatt cacaactgtc tgcatctggc gatcaatcgg ccgttgactg
     2161 aagcgaagaa gcagcactat ttggtgatgg cgcaggaaga agtcgaacgc ttgatcacgc
     2221 tcgtccaacg cacactggag ttctatcggc catcgaaagg ccgatcggct ctcacgcgcg
     2281 tcaacgatct cattgaaaat gtactggcgt tagcgaacaa gcaattggaa caggtttcga
     2341 tcaacgttca caaaaatttg gccaacgact tgccggaaat ttctgctgtg cccgatcagt
     2401 tggtacaagt ctggctgaat ctgatcatca atgcaatcga agccatgccc gacggcggcg
     2461 agctgaccat cactacttcg gccactgatg agtggctgca ggttgctatt cgggataccg
     2521 gctcaggtgt cgatccggaa gacgccgcta aaattttcga accgttttat acgacgaaag
     2581 cgaccggcac ggggttgggc ttgtcggtga gctatggtat catccaacgg catggcggcc
     2641 gcattgatgt gaacggcgtg ccgggtgaag gcaccgcatt caccgtgtcg ctgcccatcg
     2701 gtgtcattga cgatttgtga tagaagtttg aaattagagt ttggaagtta gattgatatt
     2761 tcgcgatcaa tcatccaata tctaacatcg aatttccaac agccagacaa aattatgaac
     2821 tcattgatca actcaattcc tcatctgctc attgttgacg atgaacgcgc cacgcgcgaa
     2881 tcgctggaag aaattttgac gctggaaggc tttcaagtcg cgctggctga cagcggtgaa
     2941 caggcgctcg cggtcttgcg tgaaacgccg atcgatctga tgctgctcga tctcaaaatg
     3001 aaaggcatgg acggcttgca gacgacggag accgccaagc agatcgcggc ggatacggtc
     3061 attatcatgt tgaccgcgca tggcacgttg gaatcagccg ttgcggccat gcggcgcggt
     3121 gcgtttgatt acttgctgaa gcctgcgacg gtgtctgaca tcatcgccag tgtccaacgc
     3181 ggcctcagcc atcgcgcgca gatgctgcgc caacgtgatt tagttggatt gatgcagcgg
     3241 gcgattacag aactgcaaac accggttacg caagctgagg ctgccagtca acccgatcga
     3301 tatctgcgcg cccgagggat cacgcttgat ctgcagcggc acgtagcctc ggcgcaagat
     3361 catgtgttgg atttgacttt aactgaattc aagatgctgg cctatttaat ggatcatcct
     3421 gatcgagtta tctcaccccg cgaactggtc gctgcggtgc aaggttatga agccgatgag
     3481 catgaagcgc gcgcgatcgt gcgggttcac attcgccgct tgcgccagaa gatcgagccg
     3541 gatcccgatc agcctcagca cgtcctcaac gtgcgcggcg tgggttattt atttgcggcg
     3601 agtgatactg gttaatctcc atctctaatc tctgatttgt tgggattcga gactctgcgc
     3661 agaccctcga gtccattctc aagctctaac ctccaacttc taatttccaa cccccagcgt
     3721 tataaaaccg taactggatg gaaactcgac tgatgctcga ccgatgatcg tttcgtgcta
     3781 tcctaaactg ggagcacaga tgaccgctaa aaacattttg atcgtcgacg atgaacccaa
     3841 agtcggctat accctcaaag aaagcctcga atcactcggg tcagaatacg ttgtggcgca
     3901 agcgtattca gctgaagacg cgttgagcgt gctggcccgc aagccctatg atctggtcgt
     3961 gaccgatctg cgtatgccgg gcatgaatgg actcgatctg attggccggg ttaagcgtga
     4021 cgcgcctcag acccaaacga tcttgatcac tgcctatggc tctaacgaaa tcgcggaagc
     4081 tagcgatcga ttgcaagctg cacattactt caccaaaccg tttcgtattg atgagttcgt
     4141 gcgggtcgtg gtggatacgt tgaagacgcc cgcctccaaa ggtgcatcac ccgccgcgaa
     4201 caaacatttc aagtggctgg tgcagcgctt gaatgatctg cgctttgaag tcggcgcgca
     4261 gtatgtgatc ttggccaaca tgtccggacg tctgatggcc gaatcaggca cgatcgacgg
     4321 ttttgatccg gcgctgctcg cgtccatgct cagtcgcagc ttgtcaccat cgaccgaaat
     4381 tgcccaacag ctgcgcggcg agcgcgtttt taatttggtc tatcacgaag gcgcgcgcta
     4441 tgatgtgtat gccgccaatg tcgaagaaga tttgtttatg ttgctggcgt tcgatcggcg
     4501 gcagggcgtt agtcggattg gcatggtctg gttgtatatc aagcgcgcca ttcaggaatt
     4561 gcagacacgg atcgaatcgc agggcaatcc accggaagtc ggcgggcgct tgctgcctgg
     4621 cgcgaatgaa cgaggttatt ttttgcggtg atgacttgag gaaaataaat tgcgccggcg
     4681 ttgagccggc gccttgttta tgaattaagg aatcgatttt atcgcaggaa cttgttgtag
     4741 ataagaaact ttaagtagaa cctgtcggcg aggacaccgg tggtatgtag taaatctcaa
     4801 ccgccgatct gtgacatcac ccgttgaagc gggatcactc catcggctag atcgtggccc
     4861 cacggcttgc gaaagaccag acttctgatc aatttttcca tttcttcatc gctcgcgccc
     4921 ttccgcatcg gcgtcagcag atcgccttcg tcatcacgca gcaaacacaa tcgaagtttg
     4981 ccgtcggccg tcaagcgcac gcgcgtgcag gacgcgcaga atggattggt caatgtgctg
     5041 ataaagccga tcggggccag cgcacctttc aacttgtaca accgtgcttc accatcgagc
     5101 ttggcgtctt caatcggctc cagccggcct aatcgatctt caattcgttg catgatctct
     5161 gcttgtgaaa ccgtggcatc ttgggcgaac gcagccacat cgccaaaagg catcatctcg
     5221 atgtagcgca cttgccagcg atgtgtcaac gttagcgcgg ccagatcggc cacatcatct
     5281 tcgttgaacc cgcgcgcgac aacagcgttg atcttcagcg gtgtcaggcc gtgggcatcg
     5341 gcagcgtgca cgccctccaa cacttgatcg agatggcccc aacgcgtgat gcgatgaaat
     5401 ttttcgggat tgagcgtatc gacgctgacg ttgacgcgtt cgagtcctgc ttctttcaga
     5461 ggcttcgcta actcggttaa gcgcaggccg ttggtcgtca tcgagatccg tgtgatgcct
     5521 gcgtcgcgaa tg
//
LOCUS       DASRTJ010000181         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117837,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000181 DASRTJ010000000
VERSION     DASRTJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117837"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFF70_03810"
     CDS             complement(<1..458)
                     /locus_tag="VFF70_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZY43853.1"
                     /translation="MADEEKKQTITPEMIAVAEKAIGLTFTPAERELMLDGINEQLSD
                     LEKIRSVSINNTVPSPLHFDPRLPSMQFDHQKYRSKFSRPRAPQVPETLEELAFCSIG
                     ELAALIRSRKVTSLALTRMYLDRLKRHDPVLKCVVTLTEGLAIKQARRADR"
     gene            complement(500..1375)
                     /locus_tag="VFF70_03815"
     CDS             complement(500..1375)
                     /locus_tag="VFF70_03815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZY43854.1"
                     /translation="MRLGVVFPQTEFNGNAAAIRDYAQTAEGLGFSHIVAYDHVLGAN
                     PDRPGGWKGPYTHHTPFHEVFVLLSYMAALTKSIEFATGVLILPQRQTALVAKQAATL
                     DYLSGGRFRLGVGLGWNEVEYIALNENFRTRGKRIEEQVDLLRKLFTQELVNFTGKGH
                     TIPDAGLNPLPIQRPIPIWFGGSAEAAIKRIAYIADGWMCTQRTPEIAKPVRDLLFKY
                     LEKASRSIDSFGIEARILYGDGQSDSWLSAIEQWIELGATHISINTMGSGFITPVQHI
                     DAMQKMAKAVMGKFW"
     gene            complement(1522..2385)
                     /locus_tag="VFF70_03820"
     CDS             complement(1522..2385)
                     /locus_tag="VFF70_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY43855.1"
                     /translation="MSKVAFVTDSTCNMPADLLQKHQIKMVPVYVIFGEQSYKDYIEL
                     PPAGFYARLAANKAAGKEMPKTSQPTPEDFRAVYEALGSAGYTDVLSVQLTSKSSGTY
                     QSAVLASSMVSNIKVHPVDTLTTSMAMGFMLLEAIEIIGKGGSIDEAIAKIEQMKTHS
                     SLIFTVTDVEHLVASGRTEGAEKATDAAIAVKPIVAVTDGVPKAVGTERTQNAALQKV
                     IDLTKEKMGGAKMKRMAIVNGNIADRATKWSSEAASALGYNGQPLVVDFGPALAVHFG
                     PGLLGVAAYWE"
     gene            complement(2504..2686)
                     /locus_tag="VFF70_03825"
     CDS             complement(2504..2686)
                     /locus_tag="VFF70_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43856.1"
                     /translation="MHNRRAIRWYNIDTTLVIGGQGDVHPQSRLQHIDRSTAAYSILQ
                     RRGDDKLILIDGGIHA"
     gene            2907..>3108
                     /locus_tag="VFF70_03830"
     CDS             2907..>3108
                     /locus_tag="VFF70_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43857.1"
                     /translation="MPRRPTRNDETRYTFNVNLDYDQHRLDAIEQIHFTNTYTRSISM
                     IVVNVPPAHDRARFDLRSIQLNQ"
BASE COUNT          729 a          871 c          759 g          749 t
ORIGIN      
        1 cgatcggcgc ggcgggcttg tttgatggcg aggccttcag tcaaggtcac cacgcatttc
       61 aacactggat cgtggcgctt caatcgatcg agatacatcc gcgtcaatgc cagcgaagtc
      121 actttgcgcg accgaatcaa cgccgccagt tcaccgatcg agcagaaagc tagttcctct
      181 aaagtctccg gcacttgcgg tgcgcgtgga cgagaaaact tcgatcggta tttctgatga
      241 tcgaactgca tgctgggcag acgcggatcg aaatgcagcg gcgatgggac agtgttgtta
      301 atcgagaccg atcggatttt ctccaaatcg ctcaactgct cattgatccc gtcgagcatc
      361 aactcgcgtt cggcaggggt gaaggttaag ccgatcgctt tctcggcaac tgcgatcatc
      421 tcgggagtaa tggtctgctt cttttcttcg tccgccataa ttgagcctta ttccagttgt
      481 caatcccgcg aaacacgagt taccagaact tacccatcac ggctttcgcc attttctgca
      541 tcgcatcgat atgttggacc ggtgtaataa atccggagcc catcgtgttg atcgagatgt
      601 gcgtcgcacc caattcgatc cattgctcaa tcgcggagag ccaactatcg gattggccgt
      661 cgccatataa aattctggct tcgatgccga aactatcaat cgatcgactg gctttttcga
      721 gatacttaaa gagcagatca cgtacaggct tggcaatttc cggtgtgcgc tgagtgcaca
      781 tccaaccatc ggcgatgtaa gcgatacgtt ttattgcggc ctcagctgag ccgccgaacc
      841 agatcgggat cggtcgttga atcggcagag gattcaagcc cgcatcgggg atggtgtgcc
      901 ccttacccgt gaagttcacc aactcttgag tgaacaactt gcgcagcaga tcgacctgtt
      961 cttcgatccg cttgccgcgc gtgcggaaat tttcattcaa cgcgatatat tccacttcat
     1021 tccagcccaa accaacccct aatcgaaatc gaccgccgct caagtaatct agcgtcgcgg
     1081 cttgtttagc gacgagcgca gtctgtcgtt gcgggagaat caacacgcca gtcgcaaact
     1141 cgatcgactt cgtgagcgcg gccatgtagc tgagcagtac gaatacttca tggaaaggag
     1201 tgtgatgagt gtaagggcct ttccagccgc ccggccgatc gggattagcg cccaagacat
     1261 gatcgtacgc gacgatgtgc gaaaagccca gaccttcggc agtctgggca taatcgcgga
     1321 tcgcagcggc attgccgtta aattctgttt gtggaaagac gacccctaga cgcatatcta
     1381 gctaacctcc aataaatatt ttgaagacgt ctgaccacgg accacagttg atctatattc
     1441 attatcgatc gacccctgaa caacaaatca tgatgagcaa caatgaatca actgcggtct
     1501 actgtttgct atctgcggtc atcattccca ataagcggct acaccgagca agcccggacc
     1561 gaaatgcacc gccagcgcag ggccaaaatc aacgacgagt ggctggccgt tatagcctaa
     1621 cgcgctggcc gcttcgctcg accacttcgt cgctcgatcg gcaatgttgc cgtttacaat
     1681 cgccatacgc ttcatctttg cgccacccat cttctctttg gtaagatcaa tcactttttg
     1741 cagcgcggca ttttgcgtgc gctctgtccc gacggccttc ggcaccccat cggttaccgc
     1801 cacgatcggc ttcacggcaa tagccgcatc tgtcgctttc tctgcacctt cggtgcggcc
     1861 cgatgccacg agatgctcga catccgtcac ggtgaagatc agcgaagagt gcgttttcat
     1921 ctgttcgatc ttggcgatgg cctcatcgat gctgccgccc ttgccgatga tttcgatcgc
     1981 ctccagcagc atgaaaccca tcgccatgga tgtggttaat gtatcgaccg gatgcacttt
     2041 gatattgcta accatactcg acgccaaaac cgccgactga taagtgccgg acgatttcga
     2101 agtgagctgt accgacaaga catccgtgta accggcgcta cccagcgctt cgtacaccgc
     2161 gcgaaaatct tccggcgtcg gttgcgaggt cttgggcatc tctttgccag ccgctttatt
     2221 ggcggccagc cgcgcataaa acccggccgg cggcagctcg atgtaatctt tgtacgattg
     2281 ctcgccaaag atcacataga ccggcaccat tttgatttga tgcttttgca aaagatctgc
     2341 tggcatgtta caagttgaat cggtgacgaa tgcgactttg ctcatgactg gctccttaga
     2401 ttaaaaatta ttgaaccgtg agggcgagaa gaaaaatcaa actacatctt ggcgttcttg
     2461 atttcttggc agattatctg tctacttcgt aaccggcttt gctctaggca tgaataccgc
     2521 cgtcgatcaa aatcaattta tcatcgccgc gccgctgcaa gatcgaataa gccgccgtcg
     2581 atcgatcaat gtgctgcaat cgggattggg gatgaacatc gccctggccg ccgataacaa
     2641 gagttgtgtc gatattgtac catcgaatcg cgcggcgatt gtgcaacgca gccaatcaag
     2701 atacaatcgc acggatgtta aatcgattga accatcacgc tcgatctgtg gcgctagtct
     2761 taggtgggtt aatctttttc gggtttggtg caaacccgat tgatcttcca agcctgatcg
     2821 atcccatgtt attgcgagcg cagtccttag atggcacagc ctcgagggat gtgccacgaa
     2881 gcaattcccg ccacaacccg ccgagattgc ctcgtcgtcc gactcgcaat gacgaaacac
     2941 gctatacctt caacgtcaac ctcgactacg atcagcatcg cctcgacgcg attgaacaaa
     3001 ttcacttcac caatacatac actcgatcga tctcgatgat cgttgttaac gttccacccg
     3061 cccacgatcg agcgcgattc gatctgcgca gcatacaact taatcaag
//
LOCUS       DASRTJ010000182         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_48295,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000182 DASRTJ010000000
VERSION     DASRTJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48295"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..299)
                     /locus_tag="VFF70_03835"
     CDS             complement(72..299)
                     /locus_tag="VFF70_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZY43858.1"
                     /translation="MAAKRRVQYVVEWDAERVKALRDHLGMTQIQFAEELGVRQQTIS
                     EWEVGVYKPRRSTSKYLELIAERADFKFNSK"
     gene            complement(412..885)
                     /locus_tag="VFF70_03840"
     CDS             complement(412..885)
                     /locus_tag="VFF70_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZY43859.1"
                     /translation="METHRVYRSKTDSMIGGVCGGLGAYFGIDPTLVRLIFVLMAVFG
                     GGGVLIYLILWIVIPLEGQTAATPQQTINANAQELADRARDFGKGFERSFDRSAAKRT
                     GHNGAVLFGIALIVLGGLFLLQNLLRINFSQFWPVILIVIGLAMLVPLFKRTQQQ"
     gene            complement(1010..>1719)
                     /locus_tag="VFF70_03845"
     CDS             complement(1010..>1719)
                     /locus_tag="VFF70_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZY43860.1"
                     /translation="VNWNLYPAIVKPVNQHASYGISREAIVENEHELKRRVEYVHETF
                     QSAALIEEFIDGPEFHVLVWGNTPPTVLPAVQLEYGHAADWHDRLYTYEAKFDEHSAA
                     MKKIRFLCPAMMEPRTRLSIESATLQAYRTLGCRDYARIDVRVREGQAYVIDVNANPD
                     ISSFSVAVMAAEAAGYHYDQMVTQIVRFAAERWPVQRPMLMSTQHQWAADLNRTPSES
                     EIDYGGWAQNQKPAKGS"
BASE COUNT          401 a          502 c          414 g          402 t
ORIGIN      
        1 cgccacatga ccggtgtgag aagtcaatac gcaaaaatag aactgcgcgg tcttggggga
       61 cctcgcaggt ttcatttgct attaaattta aagtctgctc gttcggcgat taactctaaa
      121 tatttggagg tcgatcggcg gggcttgtaa acgcccactt cccattcgct gatcgtttgc
      181 tggcgtacgc ccagctcttc ggcaaattgg atttgagtca tccccaaatg atcgcgcagc
      241 gctttcaccc tttcggcatc ccactcgaca acatattgaa cccggcgttt ggcagccatc
      301 aacctgcgct cctgatcggt tattagtcta gcgtgttgtc ctgtcaccgg caaacagaga
      361 gatgccccgc ttagaagtct ctcctcgatc agacttcagt atgcaggctc cttattgttg
      421 ttgcgtccgc ttgaataacg gcaccagcat cgctaaaccg atgacgatca aaatcaccgg
      481 ccagaactgg ctgaagttta ttcgcagcaa attctgaagc agaaacaaac cgcccaacac
      541 aatcaacgca atgccaaaga ggaccgcgcc attgtgtccg gtacgcttag cggccgatcg
      601 atcaaaactg cgctcaaagc ctttgccgaa gtcgcgcgcc cgatcggcta gctcctgggc
      661 attggcgttg atcgtttgct gcggggttgc ggcagtctgg ccctccagcg gaatgacgat
      721 ccacaaaatc aaatagatca aaacaccgcc gccgccaaag accgccatca acacgaagat
      781 caatcggacg agggttggat cgatcccgaa ataggcaccc aatccgccgc acactccgcc
      841 gatcatgctg tcggtcttac tacgatacac tcgatgcgtt tccattttaa gcctcctgct
      901 gattgcgact tgagtccagt cgattaggat catagcatgt ccatcaataa ttgacgggca
      961 ttcagccgac gcctgactga cgcttggccg atgcccgctc gtcccttggc tatgaacctt
     1021 tggcaggttt ttgattctgt gcccagcctc catagtctat ttcagattcg gacggtgtcc
     1081 gattgagatc agcagcccat tgatgctgcg tggacatcaa catcggccgc tgtacaggcc
     1141 agcgctcagc cgcgaaccga acgatttggg tgaccatttg atcataatga taccccgcgg
     1201 cctcagccgc catcactgcc accgagaacg aactaatatc gggattggca ttgacgtcaa
     1261 tcacatacgc ctggccttca cgcacccgta catcaatgcg cgcgtagtcg cgacagccca
     1321 gcgtgcgata tgcttgcagc gttgccgatt cgatcgatag acgcgtgcgc ggctccatca
     1381 tcgccggaca caaaaatcgg atcttcttca tggccgccga gtgttcatca aacttggctt
     1441 cgtacgtgta caaccgatca tgccaatcgg ccgcgtgacc atattccagc tggacggccg
     1501 gtaatacagt cggtggagta ttgccccaga ccaatacatg aaattccggc ccgtcgataa
     1561 actcttcaat caatgctgcc gactgaaatg tctcgtgcac atattccacg cgccgtttca
     1621 gctcgtgctc attttcaacg atcgcttcgc ggctgatgcc ataactggca tgttgattga
     1681 ccggcttgac gatggccgga taaagattcc agttcacat
//
LOCUS       DASRTJ010000183         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_117888,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000183 DASRTJ010000000
VERSION     DASRTJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117888"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFF70_03850"
     CDS             complement(<1..684)
                     /locus_tag="VFF70_03850"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZY43861.1"
                     /translation="MNTPNLAKQLNINTDLALKVLKTFIRDEVRKVGIEHVVIGLSGG
                     IDSALACYLAAKAIGTHNIFAVRLPYKSSSKDSLDHAQLVVDALGVSSETFDITDMAD
                     PLIDRTPGITEKRKGNIMARARMIVLYDRSAQYNGLVMGTSNKTELLLGYGTLFGDLA
                     SALNPIGDLYKTQVRQLAQAVGVPQPIINKAPSADLWAGQTDEKEMGVTYAEVDQLLY
                     LLIDERYSID"
     gene            complement(742..1593)
                     /locus_tag="VFF70_03855"
     CDS             complement(742..1593)
                     /locus_tag="VFF70_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZY43862.1"
                     /translation="MKINIGLAQISPKLADVRSNCELHLSFVERAAKEKVDLLVFPEL
                     SLTGYALQDASRDVARQAVPTDPLISTMLEASRRMDMVVGFAEVDDRGRLFTAGAYLS
                     QGSIVHIHRKVYLPTYGMFDEGRYFDLGDSVRAFDTRFGRMGLLICEDFWHASPPYLV
                     WLDGADVLLLTSASPGRGLNDEDRLASARFVETVNKSYAALFGSYVVHSNRVGFEDGQ
                     NFWGGSTIFDPDGELVTQAPYFEEALTVATIDLAQVNRSRNRIPLLRDERPELTLREL
                     QRIVNHG"
     gene            complement(1698..2501)
                     /locus_tag="VFF70_03860"
     CDS             complement(1698..2501)
                     /locus_tag="VFF70_03860"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZY43863.1"
                     /translation="MHPILLDWNGITIGSYAALLDLGLIVASIVVWIEARRWQIRSAI
                     WLDAILAAITIGVVAARLGYAAINFSYFKDHLYEIPRIWDGGLNWQAGLIGGSIGAWL
                     IAHRQKDQSPMKILDLLAIGVPIGIALGWIGCYLAAAAYGKEMFPGQPLYFISIDAPD
                     QYGSINPRWPSQLFGATWALVVFGLLALTRNKKWPDGMRFWIFMTLYSLGAFLIGFMR
                     ADDMPIISGWRLDQILDGALMIIGMTAVILSAHRARRISALIDPVADSN"
     gene            2600..3898
                     /locus_tag="VFF70_03865"
     CDS             2600..3898
                     /locus_tag="VFF70_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZY43864.1"
                     /translation="MCVRLMKWLMLIALIVTACAPAAPASSAIEDVRPDGQEVVVWHS
                     YEGSLRAALLTQIDEFNATNPWHIVVVPEYHGQNLQLKRELKDAISTGSTPDLAILAP
                     ADAYALSDAIVRLQRYVDNSRYGLSSDEVADMFSAMLDTTRDPKQGTLIGFPLGGEGV
                     VLVHNADRLAAHNYLTPPDSWPLFSEVCRSNTVDTTGDNKPDVFGFGFNPRADFAAAW
                     MISRGGSLLSDDGLHTTFNSDEGVKTLQTLQDTASSGCFYPTTDEDQINDFARGKVAM
                     IFALTTQLPDIFNAVQARGGFRWGVSPVPYGKRDPSLTISGPAWTMLRSTPAKQLAAW
                     LFIKWFSDTAQTVGWSQLTGLLPLRKSAGAGLANEFTTNAGFKVAFDLLSVAKAQPDI
                     PAWGNIAELLIHAVNAAVAGNDPTQLLNDAAKNADALLNQ"
     gene            3936..>4542
                     /locus_tag="VFF70_03870"
     CDS             3936..>4542
                     /locus_tag="VFF70_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZY43865.1"
                     /translation="MTDLLSVAQSAAFAAGKVLREKYPQVRVIQVKGVQDIVTDADFA
                     AQTAIVDVIKSHDPHAAILSEEGIEPDPQARLIWVVDPLDGTTNYARRLPMFGTSIAA
                     VQDGQPIAGAIYDPLRDLLFAAEIDRGATLNGVPIHASMTSEVRESIVGLDWGRGEAA
                     RSIGLSWLQRTGTQVKSIRAIGSSALALCYIACGWIDIYFHA"
BASE COUNT         1000 a         1194 c         1323 g         1025 t
ORIGIN      
        1 atcgatcgag tagcgctcat cgatcagcag atacaacaac tgatcgacct ctgcatatgt
       61 cacgcccatt tccttttcgt ccgtttgtcc ggcccacaga tcggccgatg gcgctttatt
      121 gataattggc tgcggcacgc cgaccgcttg cgcgagttgc cgcacctgcg ttttgtatag
      181 atcgccgatc ggattaagcg ccgaagcgag atcgccgaac aatgtgccat agcccagcag
      241 caattcggtt ttgttgctgg tgcccatcac caagccgtta tattgtgcag atcgatcata
      301 cagcacgatc atccgcgcgc gcgccataat gttgcctttg cgtttttcgg tgatgcccgg
      361 cgtccgatcg atcaacggat cggccatgtc ggtgatatcg aaggtctcgc tgctgacgcc
      421 cagtgcatcg acgaccagct gcgcatgatc gagactgtct tttgatgaag atttgtatgg
      481 cagccgcacg gcgaaaatat tgtgcgtgcc gatcgctttg gcggcgagat aacaggccag
      541 cgctgaatcg atgccgcccg ataggccgat gacaacgtgt tcgataccga ctttgcggac
      601 ttcatcgcga atgaaggtct ttaggacttt caacgcgaga tcggtgttga tgttgagttg
      661 tttggcgagg tttggtgtgt tcatgataga ttttttaacc acagattaca cggatgtcgc
      721 ggataagaaa ataaatcttt gtcacccgtg gtttacgatt ctttgcaatt cacgtaaggt
      781 gagttccggt cgttcgtcgc gtagcaatgg aatgcgatta cgcgatcgat tgacttgcgc
      841 tagatcgatc gtcgccacag tcagcgcttc ttcgaagtaa ggcgcttgcg tgacgagttc
      901 accatcggga tcgaagatgg tcgagccgcc ccagaaattt tggccgtctt caaagcccac
      961 gcgattggaa tgaaccacat agctaccgaa caaagccgcg tatgatttgt tgaccgtttc
     1021 gacaaagcgt gcggaagcca atcgatcttc gtcgttcaag ccgcgaccgg gactggcgga
     1081 ggtgagcagc aacacatcgg cgccgtcgag ccacacgagg taaggcggac tcgcatgcca
     1141 gaagtcttca cagatcaaca ggcccatgcg gccgaagcgc gtatcgaacg cgcgcacgct
     1201 gtcgcccaga tcgaagtagc ggccctcatc gaacatgccg taagtgggca gatacacttt
     1261 gcgatgaatg tgaacgatcg atccttgcga caagtaagct ccggccgtaa acagccggcc
     1321 tcgatcgtcc acctcggcga aaccgaccac catatccatt cggcggctgg cttcgagcat
     1381 ggtgctgatc aatggatcgg tgggcacggc ctggcgggcc acatcgcgcg aagcgtcttg
     1441 cagcgcataa cctgttaaag acagttcagg gaagaccagc agatcgactt tctctttggc
     1501 ggcgcgttcg acgaaggaga gatgcagttc gcaattggat cgcacgtccg cgagtttagg
     1561 gctgatctgc gcgaggccga tattgatttt catgaacgcc tcttggttgg tgtatagatt
     1621 acactaataa gtgtaccgcc gagcgggtga gttgtcaagc acaaatgtca ttctgaaccg
     1681 cgaagcggtg aagaatctca attgctgtcg gcaacagggt cgatcagcgc agagattctt
     1741 cgcgcccggt gggcgctcag aatgacagct gtcattccaa tgatcatcaa tgccccatca
     1801 agaatttgat ccaagcgcca acccgaaata attggcatgt catccgcgcg catgaagccg
     1861 atcaagaatg cgccgagact atagagcgtc atgaaaatcc agaagcgcat tccatccggc
     1921 cattttttgt ttcgagtaag cgctaatagg ccgaagacga ccaacgccca tgtcgcgccg
     1981 aaaagttgac tgggccagcg aggattgatc gagccatact gatcaggtgc atcgatggat
     2041 atgaaatata gcggctgtcc cggaaacatt tctttgccat aggctgctgc ggcgagataa
     2101 caaccgatcc agcccaacgc aatgccgatc ggcacaccga tcgctaagag atcaaggatc
     2161 ttcatcggcg attgatcttt ttggcgatgg gcgatcagcc acgcgccgat cgatccgccg
     2221 atcagtccgg cctgccaatt caagccgccg tcccagatgc gcgggatttc gtacagatga
     2281 tctttgaaat aagaaaaatt gatcgccgca tagcccaatc gggcggcaac gacaccgatc
     2341 gtgattgccg ccaggatcgc atcgagccag atcgccgatc ggatttgcca gcgccgcgct
     2401 tcgatccaaa caacgataga tgccacgatc agccccagat cgagcagcgc cgcgtatgat
     2461 ccgatcgtga tgccattcca atcgagcaaa atgggatgca tgcgtgaaag tatatcacca
     2521 cttgcgtttg acgcgcgatg ctcgctcggt tatactcagc cagcgttaca aagtgaattt
     2581 aggaaagaga tgctgttcca tgtgtgtgcg tttgatgaaa tggctgatgt tgatcgcgct
     2641 gatagtgact gcctgcgcgc cggctgcgcc tgcctccagc gcgatcgaag atgtccgacc
     2701 cgatgggcag gaagtggtgg tgtggcattc gtatgagggt tcgctccgcg ctgcgttgtt
     2761 gacgcaaatt gatgagttca atgcgacgaa tccgtggcac attgtggtgg tgccggaata
     2821 tcacggccag aacttgcagt tgaagcgcga actaaaagat gccatcagca ccggttccac
     2881 gcctgatctc gcgatcctcg cgccagcgga tgcgtatgct ttaagcgatg cgattgtgcg
     2941 cttgcagcgc tatgtggaca attcgcgcta tggtttgagc tccgatgaag tggcggatat
     3001 gttcagcgcg atgttggaca cgacgcgcga tccgaagcag ggcacgttga tcggttttcc
     3061 gctcggcggc gagggtgtag tgttggtcca caatgccgat cggttggcgg cgcataatta
     3121 tttgacgccg cctgactcgt ggccgttgtt cagtgaagtt tgccgcagta acaccgtgga
     3181 tacgacgggc gataacaagc cggatgtatt tggcttcggt ttcaatccgc gcgctgattt
     3241 tgcggcggcg tggatgatta gccggggcgg atcgctgttg agcgatgatg gtttgcatac
     3301 aacgttcaac agtgatgaag gcgtgaagac gctgcagaca ttgcaggaca cggcttccag
     3361 cggctgtttc tatccgacaa ccgacgagga tcaaatcaac gattttgcgc gcggcaaggt
     3421 ggcgatgatc tttgcgttga cgacgcagct gccggatatt ttcaatgccg tgcaagcgcg
     3481 cggcggtttc cgctggggcg tatcgccggt gccatatggc aagcgcgatc cctcgttgac
     3541 gatcagcggg ccggcctgga ccatgctgcg gagcacaccg gccaagcaat tagccgcctg
     3601 gttatttatc aaatggtttt ccgatacggc gcagacggtg ggctggtcgc agttgaccgg
     3661 gttgctgcca ctgcgcaaat cagccggggc gggactggct aacgagttta caacgaatgc
     3721 cggtttcaag gttgcgttcg atctgttatc ggtggccaag gcgcagccgg atattccggc
     3781 ctggggcaat attgccgagc tgctgatcca tgcggtgaat gcggccgtcg ccggcaacga
     3841 tccgacgcag ctgttgaacg acgcggccaa gaatgcggac gcgttgttga atcaataacg
     3901 aatgctcact gcaccgtgca ttaagaattt tttctatgac tgaccttctc tccgtcgctc
     3961 aatctgccgc attcgctgcc ggcaaagttc tacgcgagaa atatccgcaa gtgcgtgtta
     4021 tccaggttaa aggcgtgcaa gatattgtga ccgatgcgga ctttgccgcg caaacagcga
     4081 ttgtcgatgt gatcaagtcg cacgatcccc atgccgcgat cttatccgaa gaaggcattg
     4141 agccggatcc gcaagcccga ttgatctggg tggtcgatcc gctcgacggc acgacgaact
     4201 acgcgcgccg cttgccgatg ttcggcacgt cgatcgcggc ggtgcaagat ggtcagccga
     4261 tcgcgggtgc gatttacgat ccgctgcgcg atctgttgtt cgccgccgag atcgatcgag
     4321 gcgcaacgtt gaacggcgtg ccgattcacg cttcaatgac gagcgaggtt cgtgaatcga
     4381 tcgtgggttt ggattggggg cgcggtgagg ccgcccgctc gatcgggtta agctggctcc
     4441 agcgaacggg cacacaagtc aaatcgatcc gcgcgatcgg atcatcggct ttagcgctgt
     4501 gctatatcgc ctgcggctgg atcgatattt attttcatgc gg
//
LOCUS       DASRTJ010000184         7644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_48361,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000184 DASRTJ010000000
VERSION     DASRTJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48361"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFF70_03875"
     CDS             <1..238
                     /locus_tag="VFF70_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZY43866.1"
                     /translation="GTVHAFTVCHFGSEEFLPECPYVLALIEFEGANTLFLTRLMGVD
                     PNAASLDWIGLRVQAKYLRNSKLKPTDVYFVPKD"
     gene            416..538
                     /locus_tag="VFF70_03880"
     CDS             416..538
                     /locus_tag="VFF70_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43867.1"
                     /translation="MLAAEALTSRSAQAGQCCFITDHSATNFYDFFIPFLQALD"
     gene            590..742
                     /locus_tag="VFF70_03885"
     CDS             590..742
                     /locus_tag="VFF70_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43868.1"
                     /translation="MSESLARWGFSGDQLPTLTRYVVKSTCEDFSFTHAKATRDFGYQ
                     PIVSLE"
     gene            complement(833..1933)
                     /gene="ugpC"
                     /locus_tag="VFF70_03890"
     CDS             complement(833..1933)
                     /gene="ugpC"
                     /locus_tag="VFF70_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZY43869.1"
                     /translation="MSSVTFQNVTKRYGNVLAVDNLNLTIADQEFLVFVGPSGCGKTT
                     SLRMLAGLEDITEGNIYIGERLINDVPPKDRDIAMVFQSYALYPHMDVADNMSFALKL
                     RKTQKTEISERIRQAANVLGIDALLSRKPGQLSGGQRQRVAVGRAIVREPHVFLFDEP
                     LSNLDAKLRVQTRAEISKLHKQIGATFVYVTHDQTEAMTMGSRIAVMKDGVLQQVDTP
                     QTLYDRPGNQFVAGFIGSPSMNFFEMQLIQSDDELYAQSETFRLRLPLDRHTALKDYV
                     GKSIVVGLRPEDLYDTAYASPGIKAATIEAKVDVIELMGNEIFLYLLSGPHAITARVD
                     PRSRARVGNIVALTINLDQLHFFDKDTERTIR"
     gene            2080..3045
                     /locus_tag="VFF70_03895"
     CDS             2080..3045
                     /locus_tag="VFF70_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZY43870.1"
                     /translation="MPLTALEVQTQAQKIVDNLETVIVGKRHELELVLIGLLCQGHLL
                     IEDVPGVGKTMLARSLAQSLGCTFSRIQFTPDLLPSDVTGSSIFNPQTRQFEFRPGPI
                     MGQIVLADEINRATPKTQSALLEAMEEHQMTIDGVAHPLPKPFLVIGTQNPIEYEGTF
                     PLPEAQLDRFLLRVSLRYMSKREEIRMIEQQQFAHPLGALNAVVSGDELLALQGAVKQ
                     VFVSEAVKQYVVDIIFATRENDSLYLGSSPRGSLGLFRTGQAWAAVHGRDYVLPDDIK
                     ALAEVVLAHRVMVNPSARLKQVTPKRVIADLLQHVPQPDAELSRK"
     gene            3179..4444
                     /locus_tag="VFF70_03900"
     CDS             3179..4444
                     /locus_tag="VFF70_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZY43871.1"
                     /translation="MRQRLFLVAWVVLSAWFILIPNPLAVTGLVTLGSLLLIGYGWTR
                     SLATHVITQRVLRYSAVQVGDELEELVALNNRSVLPVIWAEFIDRSTAPGYSISGVRI
                     VGSRKTEQWRMHTSCSQRGVYTLGPWDVRLGDPFGIFEGRQAYRQQTEIVVYPPLAAL
                     PPEIAQQRRAIGDRAPLRQPVSADTINAMSTRPYVMGDALQHIHWRTSAKHDDLFVKV
                     FEPEASATMWLIPDLDATVQLGEGVDSSLEKMIVLTATIASRLLDQHLAVGVILDAAH
                     SHTVLPQAGSAHLWTILRALALVHATSDRPLAQTLLHARSIVSLRDSSVILTPSLDAE
                     WMKALPHLSGGSRMGGVEVVLLDPMSFGGSIEAEGFHSILAEQGVPAHVVRRQDIRPA
                     IGTYGAVRRWEFKILGTGRVAVMQTPRVA"
     gene            4544..6763
                     /locus_tag="VFF70_03905"
     CDS             4544..6763
                     /locus_tag="VFF70_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZY43872.1"
                     /translation="MLNSVRDNAWNQIRQGWITVLLVGVVVVSWLQVIASADWLRDTN
                     VLVSAAICGVLYGLLLASSRFRGRTALIIDLVTALGFALLVAGRVLPEMKLLTAQPFE
                     KTIWLMNARTFTLLEALRDDAQWLVTNYFPSTRLFLFLNVFAVWTAAAWLMWCVARRK
                     RALAGVLPIALLIAYYTGLGKDGPSVPLWFIGGSVLLMARTAFTYTTHDWDRRRVGYP
                     DLIGEDWAAAAATLSVAVILLAGISTPEWRSSMQRFMESLRPPPPADKTAAPVVVQVK
                     PTGDYAASFVPSMGYVGDPFPESAETIFYVTTSDSPSGMESNGVAKPPTQQHYWRGAI
                     YDRYTGTGWEQLNVSVAVSSPESIDLVAPGRYALTQQYQIVSLQDNRLFAAGQPVASK
                     DAALQAAPEDATAILPRGRIPRYEITSWAPKVSAEELEAATPMYPPEIATRYLQLPDS
                     VPQRVKDLAARLVHGAASPYDKAIRVQEYLRATYPYKLNVPAPPSNRDVVDYFLFDAP
                     GGFCSYYASAMTVLLRSQGVPARVVVGFAAGDYDGLLRRYVVPANAAHSWVEVYFPNY
                     GWIEFEPTSSRSTFEYTGKETPLPDRQPETSTARSDDAAQTVIAIGAFAVLLIGSVGF
                     AAFYLWRRQRLLQLAPEVQARKLYWQTRRSLAQLGFEASGSATPVEFLDACADRLIDH
                     PSLQESVRRVTELYIRAVFMPAPPDRSDVDSVRRSWRSTWRERVRLRWRRMKSRLEH"
     gene            7054..7185
                     /locus_tag="VFF70_03910"
     CDS             7054..7185
                     /locus_tag="VFF70_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43873.1"
                     /translation="MQGSIKKHFGDDTLQVKDAKRGAIHSHAGTGTYVSVSILEREA"
     gene            7196..>7644
                     /locus_tag="VFF70_03915"
     CDS             7196..>7644
                     /locus_tag="VFF70_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZY43874.1"
                     /translation="MTILPDQRPESLGGTIVHNIPFPNELNEETLALLKQMSPIQIEQ
                     VYSITYLHSYGQDSPFFAGLTNGVLLGSRDPQTGYTYANPRGHDMFTGQDTQWIVLPN
                     EGTVHAFTVCHFGSEEFLPECPYVLALIEFEGANTLFLTRLMGVDPNA"
BASE COUNT         1448 a         2197 c         2308 g         1691 t
ORIGIN      
        1 aggtacggtg cacgccttca cggtctgtca cttcggatcg gaagaattct tgcccgaatg
       61 tccttacgtc ctcgcgctga tcgagttcga gggagcgaac acgttgttcc tcacgcgctt
      121 aatgggcgtc gatccgaatg cagcctcgct cgattggatc ggcctgcggg tgcaggcgaa
      181 gtatctgcgg aacagcaagt tgaagccaac ggatgtgtat ttcgtgccga aggattaggg
      241 atcagttaac ccagaatcag gaaataggcg atcgcgcaaa tcgagatcgc ccactcgttt
      301 gtttaagtct gttccgaggc attgcaatag atcgctacgg tgcgaaagag gcggaccgca
      361 attgcgccac gcttgatgaa gatcgagccg gtgttcgcca acgccgtaca ccatcatgct
      421 ggcagccgag gcgttaacga gccgctccgc ccaggcgggg cagtgctgct tcatcaccga
      481 tcattccgcc accaatttct atgatttctt cattccgttc ctgcaagcgt tggattaacc
      541 cgctaacaca cgcaccattc ccgctccgat cgcgtatgcg ctggcgacgg tgtctgaatc
      601 gctggcgcgc tggggcttca gcggtgatca actgccgacg ttgacgcgct acgtggtcaa
      661 atcgacgtgc gaagattttt ccttcacgca cgctaaggcc acgcgcgact tcggctatca
      721 gccgatcgtg tcgctcgaat aggcgcaggc cgatacgatt gagtggttga aacaagcagg
      781 atttgcgaag taaccgaaac ctcccggggg gttaagtccc ccgggaggtt ttttagcgaa
      841 tggtccgttc ggtatctttg tcgaagaagt gcagctggtc aaggttgatc gttagcgcca
      901 cgatgttgcc cacgcgggcg cggctgcgcg gatcgacccg cgcggtgatg gcatgcgggc
      961 cgctgagcag atacaggaat atttcgtttc ccatcaattc gattacatcc actttggctt
     1021 cgatggtcgc ggccttgatg cccggcgaag cataggccgt gtcgtagagg tcttcggggc
     1081 gcagccccac cacgatcgat ttccccacat agtctttcag cgccgtgtgc cgatcgagcg
     1141 ggaggcgcag ccggaacgtt tcactctggg cgtagagctc atcatccgat tggatcaact
     1201 gcatctcgaa gaaattcata ctggggctgc cgatgaagcc cgccacaaat tgattgccgg
     1261 gtcgatcgta tagcgtctgc ggcgtgtcca cttgctgcaa gaccccatcc ttcatcactg
     1321 cgatgcgcga ccccatcgtc atcgcttcgg tttgatcgtg tgtcacgtag acgaaggtcg
     1381 cgccgatctg cttgtgcagc ttgctgatct ctgcgcgggt ctgcacgcgc aatttcgcgt
     1441 cgaggttgga cagcggttcg tcgaagagga acacatgcgg ctcgcgcacg atggcacggc
     1501 ccacggccac ccgctgacgc tgcccgcccg ataactgccc cggcttgcga ctcaacaacg
     1561 cgtcgatgcc tagcacgttg gcggcctgcc tgatccgttc gctgatctcg gtcttctggg
     1621 tcttgcgtaa cttcaacgca aaggacatgt tgtcggccac gtccatgtgc ggatacaacg
     1681 catacgactg gaacaccatc gcgatgtcgc gatctttagg cggcacatcg ttaatcaaac
     1741 gctcgccaat gtagatattc ccctcagtga tgtcttccag tccggccaac atgcgcaggc
     1801 tggtcgtctt gccgcagccc gacgggccga cgaagaccag aaattcctga tcggcgattg
     1861 tgaggttgag gttgtcgacg gcgaggacgt tgccgtagcg cttggtaacg ttttgaaaag
     1921 tgacgctgga catggtgctc catcagaaac cgggtatctt gtcgcgcaag ttgccaactt
     1981 gcgctacatc gctccattgt agcacgattg gcagttgcgc ctctcgcgtg gacaggctac
     2041 aattggcgcg atatttcgcc gcgaaaggat tcttcccaca tgccgctcac cgccctcgaa
     2101 gtgcagacac aggctcagaa aatcgtcgat aatctggaaa ccgtcattgt cggcaagcgc
     2161 cacgagctgg aactggtctt gatcggtttg ttgtgtcagg gccatctgtt gatcgaagat
     2221 gtgcccggcg tgggcaaaac gatgctggcg cgcagtctgg cgcagtcgct gggctgcacc
     2281 ttcagccgca tccagtttac gcccgattta ttacccagcg atgtcaccgg ctcgtcgatc
     2341 ttcaatccgc agacgcgcca gttcgagttt cgccccggcc ccatcatggg gcagatcgtg
     2401 ttggccgacg agatcaatcg ggccacaccc aagacgcaat cggctttgtt ggaagcgatg
     2461 gaagaacacc agatgacgat cgacggcgtg gcgcatccgc tgccgaagcc ctttctggtc
     2521 atcggcaccc aaaacccgat cgagtatgaa ggcactttcc cgctgcccga agcgcagctc
     2581 gatcgctttc tgttacgcgt gagtctgagg tacatgtcga agcgcgaaga gatacgcatg
     2641 atcgaacagc agcaattcgc ccatccgctc ggggcgttga atgccgtcgt ctctggcgat
     2701 gaactgttgg cgttgcaagg cgcggtgaag caagtgtttg tctcggaggc cgtgaagcag
     2761 tacgtggtcg atatcatctt cgccacgcgt gaaaacgaca gtctgtatct tggatcgagt
     2821 ccgcgcggca gtttgggctt attccgcacg gggcaggcgt gggcggcggt gcatggccgc
     2881 gattacgtgc tgcccgatga catcaaggcg ctggccgaag tggtactggc gcatcgtgtg
     2941 atggtgaatc cctcggcgcg tttgaagcag gtgacgccca agcgcgtgat tgccgatctg
     3001 ttgcagcacg tgccgcagcc cgatgcggaa ttgtcaagaa agtaaagcgt gaaacgcggg
     3061 agcactgcac cgtatgtgta aagcgtaaaa cgtgatgata aagatagtga ggcaggcaag
     3121 caaaagaatc acccgactct ggcgcattgc gcggcctgaa tgcgaggtca gcattcacgt
     3181 gcgtcagcgt ctcttcctcg ttgcgtgggt ggtgttgtcg gcgtggttta tcctcatacc
     3241 caacccgctt gcggtgacgg gcctcgtcac gctgggcagc ctgctgctga tcggttacgg
     3301 ctggacgcgc tcgctcgcta cgcatgtgat cactcagcgc gtgctgcgct actcggccgt
     3361 gcaagtgggt gatgaactgg aagagctggt ggcactcaac aatcgatcgg tgttgcccgt
     3421 gatctgggca gaattcatcg atcgatccac cgcacccggc tattcgatct cgggcgtgcg
     3481 gattgtgggc agtcgcaaaa cagagcagtg gcgcatgcat accagctgct cgcagcgcgg
     3541 tgtgtataca ctcggtccgt gggatgtgcg actgggcgat ccctttggaa tctttgaggg
     3601 tcggcaggct tatcggcagc aaaccgagat tgtggtgtat ccgccgttag cggccctgcc
     3661 gccggagatc gcgcagcagc gccgggcgat cggcgatcgc gctccgttgc ggcagcccgt
     3721 gtcggcggac acgatcaatg ccatgtcgac gcgcccctac gtcatgggcg atgcgctgca
     3781 gcacatccac tggcgcacca gcgccaaaca cgatgatctg tttgtgaagg tctttgagcc
     3841 ggaagcgtct gcgacgatgt ggctgattcc cgatctcgat gcaacggtgc agctgggcga
     3901 gggcgtggac tcttcgctgg aaaaaatgat cgtcctcacg gcgactatcg catctcgttt
     3961 gttggatcaa catctggccg tgggcgtgat tctggacgcg gcgcatagtc acacggtttt
     4021 gccacaagcg ggatcggcgc acctatggac gattctgcgc gcactggcgc tggtgcatgc
     4081 cacctccgat cggcctctgg cacaaacgtt gctccatgcc cgctcgatcg tctctctgcg
     4141 cgattcgtcg gtgatcctga cgccatcact tgatgccgaa tggatgaagg cgctgccgca
     4201 tctttccggc ggctcgcgga tgggtggcgt cgaagtggtg ctgctcgatc caatgtcgtt
     4261 cggtggctcg atcgaggccg agggcttcca ctcgatattg gccgagcaag gcgtgcccgc
     4321 acatgtcgtg cgccggcagg atattcgacc cgcgatcggc acctatggcg ctgtgcgtcg
     4381 ttgggaattc aagatcttgg gcacgggccg tgtggccgtg atgcagacgc cgcgtgtggc
     4441 ttaggcctca cccctgacct ctttcctgaa atcgtccttc gactccgctg cgctccactc
     4501 aggatgcgat ttcagacgag agggggtgag gcattcaata actatgctta attctgttcg
     4561 tgacaacgcg tggaatcaaa tcaggcaagg ctggatcacc gtcctattgg tcggcgtggt
     4621 cgtagtgagc tggctgcagg tgatcgcctc cgccgattgg ctgcgcgaca ccaacgtgct
     4681 ggtctcggcc gcgatctgcg gtgtgttgta cggcttgctg ttagcctcgt cccgttttcg
     4741 cggacgcacc gcgttgatca tcgatctggt cacggcgctg ggctttgcgc tgttggtggc
     4801 ggggcgcgtg ctgcctgaaa tgaagctgct caccgcgcag cctttcgaga aaacgatctg
     4861 gttgatgaat gcgcgcacct tcacgctgct ggaagcgctg cgggatgatg cgcagtggct
     4921 ggtcacgaat tactttccca gcacgcgcct gtttctgttt ctgaatgttt tcgcagtatg
     4981 gactgcggcg gcgtggttga tgtggtgtgt ggcacgtcgt aaacgcgcgc tggccggtgt
     5041 gttgccgatt gcattgctca tcgcttatta caccggcctc ggaaaagatg ggccgtcggt
     5101 accgctatgg ttcatcgggg gcagtgtgct gttgatggcg cggacggcgt tcacttacac
     5161 cactcacgat tgggatcgac gccgagtcgg ctatcccgat ctcatcggcg aagactgggc
     5221 ggcggccgct gccacgttgt cagtggcagt gattctgttg gccggtatca gcacaccgga
     5281 atggcggagc tcgatgcagc gcttcatgga gtcgctgcgt ccgcctccgc ctgccgataa
     5341 aaccgctgcc cctgttgtcg ttcaagtcaa acccaccggc gattacgcgg ccagcttcgt
     5401 gcccagcatg ggttacgtgg gcgatccgtt tcccgaatct gccgaaacaa ttttctatgt
     5461 cacaaccagt gattcgccct cgggcatgga gagcaatggc gtggcgaagc cgcccacgca
     5521 gcagcactac tggcggggcg cgatctacga tcggtacacc ggcactggct gggagcagct
     5581 gaacgtcagc gttgcggtat cgtcgcctga atcgatcgac ttggttgcgc ccggccgcta
     5641 tgcgttgacc cagcaatatc agattgtttc gctgcaagac aatcgcctgt tcgcggccgg
     5701 gcagcctgtc gccagcaaag atgctgcgtt gcaagccgca cccgaggatg ccaccgcgat
     5761 cttgccgcgc ggccgcattc ccagatatga gatcacgtcg tgggcgccta aagtttcggc
     5821 ggaagaattg gaagccgcta cgcctatgta tccgccagag attgccacgc gctatttgca
     5881 gctgcctgac agtgtgccgc agcgcgtgaa agatttagcg gcgcgtctgg tgcatggcgc
     5941 ggcgtcaccg tatgacaagg ctattcgcgt tcaagaatat ctacgcgcca cctatcccta
     6001 caagctcaac gtgcctgcgc caccgtccaa ccgcgatgtg gtcgattact ttctctttga
     6061 tgcaccgggc ggcttttgca gctactacgc ttcggcgatg acggtcctgc tccgttcgca
     6121 aggcgtgcct gcgcgcgtgg tcgtgggttt tgccgcgggc gattacgatg gcctgctgcg
     6181 ccgctacgta gtgcccgcca acgccgcgca ttcgtgggtg gaagtatatt tccctaatta
     6241 cggctggatc gaattcgagc cgacttcatc tcgatcgact ttcgagtaca cgggcaaaga
     6301 aaccccgctg cccgatcggc aaccggaaac ctcaacggct cgatcggatg atgccgccca
     6361 gacggtgatc gcgatcggcg cgttcgcggt gttattgatc ggcagtgtgg ggttcgccgc
     6421 gttttatctg tggcgacgtc agcggctgtt gcagttggcg cccgaagttc aagcgcggaa
     6481 gttgtactgg cagacgcggc gatcgttagc gcagctgggc ttcgaagcgt cgggcagcgc
     6541 cacgcccgtt gaatttctag acgcctgcgc tgatcgcttg atcgatcacc cgagcttgca
     6601 ggaaagtgtg cggcgtgtga ctgagctcta catccgcgcc gtgttcatgc ccgcgccgcc
     6661 cgatcgatcg gatgtggatt ccgtgcggcg atcgtggcgc tccacgtggc gtgagcgagt
     6721 gaggttgcgc tggcggcgca tgaagtcacg gcttgagcac tgaccctgtt cagagaagga
     6781 acgactcaat ctgcgtcact gcgagcgctt tcccgcgaag caatctcctg gccgatcagc
     6841 acaggttgcc aatcaggcaa gagattgctt tgttgctgcc cttcgactcc gctgcgctct
     6901 tgctcagaaa cttacgtgct cacagcgccg cccccggcgc ggacgtcggc ggtgccgtcc
     6961 agtaaattcg tttggcacat tcgatagaca gatcgatctc tctggctctt ccgggttgag
     7021 tgggacgcta cgtagccttg cgaggattgg cagttgcaag gatcgattaa gaaacatttc
     7081 ggcgacgata ctttgcaagt gaaagatgcg aagcgcggcg cgatccattc gcacgcaggc
     7141 accggcacat atgtgagtgt ttcgatcttg gaacgagaag cataaggaga tcatcatgac
     7201 catcctacct gatcaacgcc ccgaatccct cggtggcacc atcgtccaca acatcccgtt
     7261 tccgaatgaa ctcaacgagg aaacactcgc gctgctcaag cagatgtcgc cgattcagat
     7321 cgagcaagtg tattcgatta cttacttgca ttcatacggg caagactcgc ctttcttcgc
     7381 cggattgacc aacggcgtcc tgctcggctc gcgcgatccg caaaccggct acacttacgc
     7441 caatccgcgc ggccacgaca tgtttaccgg tcaagacacg cagtggattg tgttgccgaa
     7501 cgaaggtacg gtgcacgcct tcacggtctg tcacttcgga tcggaagaat tcttgcccga
     7561 atgtccttac gtcctcgcgc tgatcgagtt cgagggagcg aacacgttgt tcctcacgcg
     7621 cttaatgggc gtcgatccga atgc
//
LOCUS       DASRTJ010000185         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_48370,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000185 DASRTJ010000000
VERSION     DASRTJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48370"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="VFF70_03920"
     CDS             complement(<1..1808)
                     /locus_tag="VFF70_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43875.1"
                     /translation="MAASSPNLLPIRDGGRRFSRPLVWLFLAVTLAIFMLAPAFALHW
                     SETVPFPGVLVGQTFIVSDSSGPDWGPTPQLAVLDRIIAIDGQPITTQSEYEHALITA
                     NNQPNHRVQITFERSIALNPNECGEVVRVGIRRCEVTRPVRPFNANDDLRLFGLPYLI
                     GLCYLAIGLWVFRQRSTQPSALAVTFFCCSVGIALATTFDQTSTNVLMPMYVLSLGLV
                     SAALISLGLVFPQQPRVIDRQPLLRYLSYLPGVVISLVTISKLYDVLDPWDYINFFPA
                     LFILIAAGIVIFLVALAYRRIRADSPVVKQQSRIILWGALVAFTPFLVWIILQSINPA
                     TPFLPLLYLTPLVLFPIALALSLLRFNLFDIDHAIVGGLTFLILSLLVIAAYMALLTI
                     SSLLLQSQMAFENSPVLQGLFVLLIAVALDPLRHRIQRSVDRLFFRNRIDPQDLLQTY
                     SRRLTENADLPSLVSLIRQQVGDALRPELLHIYLLDSRTHAYNAQPNPNLPKMPAMVA
                     RLAADSPLAIWLKEEKGLRYLQTGRPLPESLEPDQARVEAIGAVLYVPFAGRNQLNGW
                     FLTKRQPAIELISASKRHIQDRTNRFNTRLIWFQTFR"
BASE COUNT          554 a          521 c          595 g          420 t
ORIGIN      
        1 cggaaagttt ggaaccagat caagcgcgtg ttgaagcgat tggtgcggtc ttgtatgtgc
       61 cgtttgctgg ccgaaatcag ctcaatggct ggttggcgct tggtcagaaa ccagccattg
      121 agctgatttc ggccagcaaa cggcacatac aagaccgcac caatcgcttc aacacgcgct
      181 tgatctggtt ccaaactttc cggcagtgga cggcctgttt gcagataacg caaccctttc
      241 tcttctttca accagatcgc aagcggacta tcggcagcca gacgggcgac catcgccggc
      301 atcttgggca gattgggatt cggttgcgca ttgtacgcgt gtgtacgcga gtccaacaaa
      361 taaatgtgca gcagctccgg acgcaaggca tcccccactt gctgtcgtat caaactcacc
      421 agactgggca ggtcggcatt ctcggtcaac cggcggctat aggtttgcaa caagtcctgt
      481 ggatcgatcc ggttgcggaa gaacaagcgg tcaacgctgc gttgaatgcg atggcgcagc
      541 gggtcgagcg ccaccgcgat caataacaca aatagacctt gtaagactgg actgttctcg
      601 aaggccatct gcgattgcag caatagcgaa ctaatggtca gcagcgccat atacgcggct
      661 atcactagca agctcaagat caaaaaagtt agcccgccaa caatggcgtg atcgatgtca
      721 aataaattga agcgcagcaa agacaaggcg agtgcgatcg ggaacaaaac caacggcgtc
      781 agataaagca acggcaggaa tggggtagcc ggattgatcg attgcagaat gatccagact
      841 aaaaagggtg taaaagcgac gagtgcaccc cacaagatga tgcggctttg ttgtttcaca
      901 accggcgagt cagcccgaat gcggcgataa gccagcgcga ccaaaaagat tacaatgccc
      961 gcggcgatga ggatgaatag cgccgggaag aagttgatgt aatcccaggg atcaagcaca
     1021 tcatataact ttgaaatcgt gacgaggctg atcacaacgc cgggcagata acttaagtaa
     1081 cgcaataagg gctggcgatc gatgacgcgc ggctgttgcg gaaagaccag gcccagtgaa
     1141 atcagcgcag cgctgaccag gcctaaactg agcacataca tgggcattaa gacattggtg
     1201 ctggtttgat cgaaggtagt ggccagcgcg atacccaccg aacagcagaa gaaagtcaca
     1261 gccagcgccg agggttgagt cgatcgctgg cgaaagaccc acaaaccgat ggctaggtag
     1321 cataatccga taagataggg cagtccgaag agccgcaggt catcgttcgc gttaaaaggt
     1381 cggacgggac gagtgacttc acaccgacgg atgccgacgc gtacgacctc gccgcattcg
     1441 ttgggattca aggcgatcga tcgttcaaag gtgatctgca ctcggtgatt gggctgattg
     1501 ttcgcggtga tgagggcatg ctcatattcg ctttgagtgg tgatcggctg gccgtcaatc
     1561 gcaatgatcc gatcgagcac agctaactgc ggggtgggtc cccaatcggg accgcttgaa
     1621 tcgctaacaa taaaagtttg tcccaccagc acgccgggga atggaacggt ctcgctccag
     1681 tgaagtgcga aagccggggc cagcatgaag atcgccagcg tgacagccaa aaacaaccac
     1741 accaacggtc gagagaagcg tcgaccacca tcgcgaatgg gaagcaggtt aggtgagctg
     1801 gccgccatac cgattttcct gtttggttga agctagacgg tgaacgaagt tttgaagtga
     1861 gttgaatttt agcataggca tatgcatttg taaacttgcc gaatggttaa atcgcaactc
     1921 tcctccattc ggcgtgggtc aatagtccgg cgatgtttgc gacgcgacgc cgcgtgttgt
     1981 ataatcctca ttcgatggtc attctcctat tcgcatcgaa tttctgcgag gaagaactac
     2041 atgccgaaaa agattcgcgt gctcatcgcc aagcccggct tggatggaca
//
LOCUS       DASRTJ010000186         5378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_313289,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000186 DASRTJ010000000
VERSION     DASRTJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313289"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFF70_03925"
     CDS             complement(<1..463)
                     /locus_tag="VFF70_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43876.1"
                     /translation="MNPASPMRNFDPRQVAYYEKESWVAYYQRRWFRLLRLLIGLIRS
                     TFGLSLLQAMYISIPSTRAQIAFAPQDNDVPKAIDQMRRFYAYIQKVHHEDFDPVEAA
                     RLEVNWWVVHRKHFGHSDNPEVIEAVANLYASAYQVPIDRVRGAATHRAQAM"
     gene            complement(657..1241)
                     /locus_tag="VFF70_03930"
     CDS             complement(657..1241)
                     /locus_tag="VFF70_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43877.1"
                     /translation="MKNTAFIRLASVCAILAGIGGVLYGVAFVILGNPLLYSVLLMLA
                     GLLTSVVLVALYDQLHESDSAWSMLAILIGLIGSIGSVIHGGYDLANAIHPPVTLNAD
                     LPSQIDPRGLLTFGLLGTAVLIFSALMRRGDQFPKNLAAVGLLLGVLMIVIYLGRLII
                     LDPTNIIVRIALLLGVIANTVWFIWLGLTWRRAA"
     gene            complement(1296..1769)
                     /locus_tag="VFF70_03935"
     CDS             complement(1296..1769)
                     /locus_tag="VFF70_03935"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZY43878.1"
                     /translation="MNVPSIFKFLHILSMFAGVGASTGTLLLVRRVARTNNATAIKTL
                     LGAARTIIAATPILFGIGAIFGLLTGATSSFDFFKPWLLIAYTIFVVLLVVGARISAP
                     WAMRVGKAAAMNQGDAPSAELQAATADQSIDTAFWITMVLLVVVIFDMIFKPFGI"
     gene            1820..2707
                     /locus_tag="VFF70_03940"
     CDS             1820..2707
                     /locus_tag="VFF70_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZY43879.1"
                     /translation="MAAAREYTPIAARVQKKQAPTRRNYFDCAVFTLPNPLWGNKMMS
                     EIEFQVDTRGIGQLIVNRPEVRNALSWSAMRSFADAVERAHDTPELRVLIITGAGRSF
                     ISGGDIQELQDYPTRADGLRLATIMGDALARLEALPCPTIAAINGPARGGGAEVAVAC
                     DLRIMSEDADIGFVHSQLGIATAWGGGQRLLRAVGYSRALDLLTTGRVLTSTEALTLR
                     LINQIVPANEAVKEAIKIAEQIAVNPPHATQAVKRILRISLSHTEGLAMEAERAEFPP
                     LWDTEFRREAVRKFLNRGK"
     gene            complement(2761..3483)
                     /locus_tag="VFF70_03945"
     CDS             complement(2761..3483)
                     /locus_tag="VFF70_03945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZY43880.1"
                     /translation="MIAAITFDFWNTLYRPAYAGPWRMRRLIETLENNGLGVEPDRLA
                     AADQVARDEWNRAWIEDYRTLSSADWLQIMFGAVQVQLPASEFETLAHHFDRGLLEDK
                     PGPTLVTGAADLLHRLAPRYRLGMISDAGLSSGQTLRDVLRRDDIFHYFDCLTYSDEI
                     GVSKPHARAFTTTLACLEAVAANTVHIGDLTRTDIAGAKSIGLRTVRFAEIYDDPDRS
                     VEPDAVVYSLVEFEKWLLDQTN"
     gene            complement(3480..4745)
                     /locus_tag="VFF70_03950"
     CDS             complement(3480..4745)
                     /locus_tag="VFF70_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZY43881.1"
                     /translation="MMRLKYILLIALLVSACAPTATPQPTSSATTVLGSPAPKQTQVP
                     AQTPAETPVIVPNTPISAPVTGPIQIDLVLMVSGITNPTNLVSANDDSDRLFVTAKPG
                     QVQIISKDQLLSQPFLDISDLVDLKGSDERGLLGLAFSPNYTTDGFFYLDYTRQPDGA
                     TVIARYSVSKDDPNLADPNSGVTILKIDQPQANHNGGQLAFGLDGYLYIGMGDGGGGG
                     DQHGTIGNGQNLNKLLGKILRIDVRNTDRYAIPSSNPFGTEVWAYGLRNPWRFSFDRS
                     TGDLYIADVGQNTYEEIDFQPAASHGGENYGWRIMEAVDCYDPGNACDPTQFVSPVTE
                     YTHDQGCAVVGGYVYRGQKYPQWQGLYFFADYCSGTIWSLQRDASSQWQIIRRLDSSL
                     NISSFGQDQAGELYVVGHGDGTIYRLAAQ"
     gene            complement(4838..>5378)
                     /locus_tag="VFF70_03955"
     CDS             complement(4838..>5378)
                     /locus_tag="VFF70_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43882.1"
                     /translation="CGTPSKVTISGPGIEGMLVVKDAKVTASLGVDQFFGAENRQPID
                     PPANPGGGYDLYRSWDYESDHFGNDHLRYYFNASGAGAIFYTQDSLELAKQIGMENMI
                     DHWFAATTQEDAIMQRVFKSINITPQASTSTASATIASPTNSSVSIGWPIGLAVGAGI
                     MIGGLLVYNRRRVRSNIVT"
BASE COUNT         1321 a         1461 c         1463 g         1133 t
ORIGIN      
        1 ccatcgcctg cgcgcgatga gttgctgcgc cacgcacccg atcgatcggc acttgatacg
       61 cgctggcata caaattggcc acggcctcga tcacttcggg attatcactg tgcccgaaat
      121 gttttcgatg cacgacccac caattcactt cgagccgcgc ggcttcgact ggatcgaaat
      181 cttcatgatg aactttctgg atataagcat agaagcgccg catctgatcg atcgccttcg
      241 gtacatcatt gtcctgaggt gcgaaggcga tctgcgcgcg cgtcgatggg atgctgatat
      301 acatcgcttg caacagcgac aagccaaacg tcgatcggat caagccgatc agcaaacgca
      361 gcaatcgaaa ccaacgccgc tgataataag cgacccaact ttctttttca taataagcta
      421 cttggcgcgg atcgaagttc cgcatagggc tggctgggtt catgggttaa tgttcgagcg
      481 ctttttgcga gaaatctcga cgatcatgag caagacttgt tcagcgtggc cgaaacactt
      541 gctccatgat gcggagcggt gcaggtgttt ctcacttcga ccgaaatgac aagcaaagtt
      601 ctaatttgat atgaacacga caacacagcg gcaacgccta gaagattacg tggagatcaa
      661 gcggcccgcc gccaggtcaa tccgagccag ataaaccaaa cggtattggc aatgaccccc
      721 agcaacaaag caatccgcac gatgatgttc gtcggatcaa gaatgatcaa gcggcccaga
      781 tagatcacga tcatcaatac gcccaaaagc aaaccgacag ctgccaggtt cttggggaac
      841 tgatcgccgc gtcgcattaa cgccgagaaa atcagcacag ccgtgcccag caagccaaac
      901 gtcagcaagc cgcgtggatc gatctggctg ggcagatcgg cattcagcgt aaccggcgga
      961 tggatcgcgt tcgccaaatc gtagccgcca tggatcacag acccgatcga gccgattagc
     1021 ccgatcagga tcgcgagcat agaccaggcc gaatcggatt cgtgcagttg atcgtatagc
     1081 gcaactagca cgaccgaagt caacaatcca gccaacatca acaagacaga gtacaacagt
     1141 ggattgccca aaataacgaa cgcgactcca tacagcaccc caccgatgcc ggctagaatc
     1201 gcgcagacac tcgccaaacg aatgaacgcc gtgttcttca ttttgccctc atgctgaaaa
     1261 taaaatgagc cggaggcaat ctccgactca agttcttaaa tgccgaatgg cttgaagatc
     1321 atatcgaaga tgacgacgac cagcagcacc atggtgatcc agaatgcggt gtcgatgctc
     1381 tggtccgccg ttgctgcctg caattcggcg gagggcgcat cgccttgatt catcgcggcg
     1441 gctttgccta cgcgcatcgc ccacggtgcg ctaatgcgcg caccgacgac caacaacacg
     1501 acgaagatcg tataggcgat gagcagccac ggcttaaaaa aatcaaatga actggtggcc
     1561 ccggtcaaca aaccgaaaat cgcgccgatc ccaaacagaa tcggcgtggc ggcgatgatc
     1621 gttcgcgcgg cgccaagcag cgtcttgatc gccgtggcat tattcgtgcg cgccacacgc
     1681 cgcaccaaca gcagagtccc ggtcgatgcg ccgacaccgg caaacatgga aagaatgtgc
     1741 aaaaatttga agatgctagg cacgttcatg ggattagtct ccttagtttg aattgggatc
     1801 gattgagaga tcgatcgtgt tggctgccgc gcgagagtat actccaattg ccgcgcgggt
     1861 gcaaaaaaag caagcaccca cgcggcgcaa ctattttgac tgcgccgtgt tcactctgcc
     1921 aaatccattg tggggaaata agatgatgtc tgaaattgaa tttcaagtcg atacgcgtgg
     1981 catcgggcaa ttgatcgtca atcgaccgga ggtacgcaat gccttgagct ggagcgcgat
     2041 gcgatcattc gccgatgcag tcgagcgcgc gcacgacacg cccgagctgc gtgttttgat
     2101 catcaccggc gcaggtcgat cattcattag cggcggcgat attcaagagt tgcaagatta
     2161 tccgacacgc gccgatgggc tgcgcctggc cacgatcatg ggtgatgcct tagcccgatt
     2221 ggaagcactg ccctgcccga cgatcgcggc gattaacggt ccggcgcgcg gcggcggcgc
     2281 ggaagtggcc gtcgcgtgtg atctgcgcat catgtcggaa gatgccgaca tcggctttgt
     2341 gcacagccaa ttaggcatcg ctacggcgtg gggtggcggg caacgtttgc tgcgcgccgt
     2401 cgggtattca cgcgcgcttg atctactgac caccggtcga gtgttaactt caactgaagc
     2461 attgaccctg cgactgatca atcaaatcgt gccagcgaat gaagccgtga aggaagcgat
     2521 caaaatcgct gaacagattg cagtgaatcc tccccatgcc acacaggccg tcaagcgaat
     2581 tttgcgaatc agcctatcac acaccgaagg cctcgccatg gaagccgagc gggctgagtt
     2641 cccgcccttg tgggacacag agtttcgccg agaggctgtc cgaaagtttt tgaatagagg
     2701 gaagtaaaga gtgctgagtg ataaggtgag acgtgatgcg cgagaatcat ttgtcacgca
     2761 tcagtttgtt tgatcgagca gccacttttc aaattcaacc aacgaataga ccaccgcatc
     2821 gggctcaacc gatcgatcgg ggtcgtcgta aatttcggca aagcgcacgg tgcgcaaccc
     2881 gatcgattta gcgccagcga tgtcggtgcg agtcagatcg ccaatgtgaa ccgtattggc
     2941 ggcgacagcc tcgaggcaag ctaaggtggt ggtaaacgcg cgcgcatgcg gcttgctgac
     3001 gccgatctcg tccgagtacg tgaggcaatc gaagtaatgg aaaatatcat cgcgccgcag
     3061 aacgtcacgc aaagtttggc cgctcgacag gcccgcatcc gaaatcatac ccaaacgata
     3121 acgcggcgcc aggcggtgca gtagatccgc cgcgccagta accagcgtcg gacccggctt
     3181 gtcctctaac aaaccacgat cgaaatgatg agctaacgtc tcgaattcag acgcgggcag
     3241 ttgcacttgc acagcgccaa acatgatctg caaccaatcg gccgagctta acgttcgata
     3301 gtcttcgatc caggcacgat tccattcatc gcgcgcaact tgatcggccg ctgcaagccg
     3361 atcgggttca acacccagcc cattgttctc cagcgtctcg atcaaacgcc gcatccgcca
     3421 gggccccgca tacgccggtc gatacagcgt gttccaaaaa tcgaatgtga ttgcagcgat
     3481 cattgagcag ccagccgata gatcgtccca tcgccgtgcc cgacgacata cagttcgccc
     3541 gcttgatctt ggccgaatga gctgatattc aagctggaat ccagccgccg gattatttgc
     3601 cattggcttg acgcgtcgcg ctgcaaagac caaatggtgc cgctgcaata atcggcgaag
     3661 aaatagagac cttgccactg cgggtatttt tggccgcgat acacgtagcc gccgacaacc
     3721 gcgcagcctt gatcgtgcgt gtattcggtg acgggcgaaa caaattgagt ggggtcgcag
     3781 gcgttaccgg gatcataaca atccacagct tccatgatcc gccagccgta attttctccg
     3841 ccatgactcg cggccggttg aaaatcgatc tcttcgtaag tattttgtcc gacatcagcg
     3901 atgtacagat cgccagtcga tcgatcgaac gagaagcgcc acggattgcg cagaccatag
     3961 gcccagactt cagtgccaaa tggattcgat gacgggatcg catagcgatc agtgttgcgc
     4021 acatcgatgc gcaagatttt gccaagcaat ttattcaggt tttgtccgtt gccgattgtt
     4081 ccgtgttgat cgcctccgcc gccgccatcg cccataccga tgtataaata gccatccagc
     4141 ccgaaggcta attgtccacc gttgtgattc gcttgaggtt gatcgatttt caagatggtc
     4201 acaccggaat ttggatcggc cagattggga tcatcttttg aaacgctata ccgggcgatg
     4261 accgtcgcgc catcgggctg gcgggtgtaa tcgagataga aaaatccgtc ggtggtgtaa
     4321 tttgggctga aggctaggcc caacaagcct cgttcgtcgc tgcccttgag atcgactaga
     4381 tcgctaatat caagaaaagg ctgagataag agttggtctt ttgaaatgat ctgaacttga
     4441 ccgggcttgg ccgtaacgaa caatcgatcg ctatcatcgt tggcgctgac caagttggtg
     4501 ggattcgtga tgccggagac catgaggacg agatcgatct ggatcggtcc ggtcacaggc
     4561 gctgagatcg gcgtattcgg aacgatgacg ggagtttcag caggagtttg agctggaact
     4621 tgagtttgct tgggcgcagg cgatcccaaa actgttgtgg ccgatgaagt gggctgcggg
     4681 gtcgccgtcg gtgcacaggc gctgacgagt aacgcaatga gtaatatata ttttaatcgc
     4741 atcatcttga ctccaaaaat tttatacgct gagattgtac cagacttgaa aacaaaatgg
     4801 ccggagggta atcctccggc caagtatggt tgatcgatca ggtgacgata tttgatcgaa
     4861 cgcgtctgcg gttgtagacc agcagaccgc cgatcattat accggctcca acggccaaac
     4921 cgatcggcca accaatcgaa accgaactgt tcgtcgggct ggcgatcgtc gcactcgcag
     4981 ttgaggttga cgcttgcggc gtgatgttga tcgatttgaa tacgcgctgc atgatcgcat
     5041 cttcttgtgt ggtggccgcg aaccaatgat cgatcatgtt ttccatgccg atctgtttag
     5101 ccagttcaag gctatcctgc gtgtagaaaa tagcacccgc gcccgatgca ttgaaatagt
     5161 agcgcagatg atcattgccg aagtgatccg attcgtaatc ccacgatcga tagagatcat
     5221 agcccccgcc cggattcgcc ggtggatcaa tcggctggcg gttttccgcg ccgaagaatt
     5281 gatcgacacc caatgacgca gtaactttcg catccttcac tacgagcatg ccttcaatgc
     5341 cggggccgct aatcgtcact ttgctcggcg ttccgcag
//
LOCUS       DASRTJ010000187         5770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_10505,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000187 DASRTJ010000000
VERSION     DASRTJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10505"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VFF70_03960"
     CDS             <1..1355
                     /locus_tag="VFF70_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43883.1"
                     /translation="FEKGLDLVYTIDDHVPPVIQGDVTRLRQIILNLLSNAIKFTGKG
                     EVILEVMEDRSREAAGGQSGRGSSGSLDFGLHFSVKDSGLGIPADRMNRLFQSFSQVD
                     ASTARKYGGTGLGLAISKRLSELMGGSMWAESEGIPGHGATFHFNIQTRAAKAPEQTR
                     RDLRGAQPQLKDKRVLIVDDNDTNRRIISLQLQKWGMLTRDTASPREALKWLEHGDPF
                     DVAILDMHMPEMDGIELAKEIRKQRDAKKLPLALFTSLGRREADAESIGFAAHLNKPL
                     KPSTLFDAMIGIFSAEGTPRAAPAVSAKPQLDPNMAQRLPLRILLAEDNAVNQKLALR
                     LLEQMGYRADVAGNGLEAIEAVERQTYDVILMDVQMPELDGLDATREIRKLKVAAQPR
                     IVAMTANAMQGDREMCLAAGMDDYISKPIHIEELVSALQQTKRSMAEVQGHNGRPQQL
                     "
     gene            1476..1730
                     /locus_tag="VFF70_03965"
     CDS             1476..1730
                     /locus_tag="VFF70_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZY43884.1"
                     /translation="MAKLKLDLHDIYNRGDQIDGELDRIIHEAIDRKIDLVEIIPGKG
                     SGQLKKKVLRFLNQSHIRQLYHRVEKDDKNFGRIFIHFRH"
     gene            1804..2157
                     /locus_tag="VFF70_03970"
     CDS             1804..2157
                     /locus_tag="VFF70_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZY43885.1"
                     /translation="MADPVIDPTTFQSLIAGTDSAFVMELIDTFLDDAPTMLNDLRQS
                     LAEKNAEVFRRAAHSLKSNSASFGALHLSTQAKELELLGKAGDLAQVGDKLTALTTEF
                     DRVQVALRNWQHGSK"
     gene            2144..3208
                     /locus_tag="VFF70_03975"
     CDS             2144..3208
                     /locus_tag="VFF70_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HZY43886.1"
                     /translation="MDRSKGYTGRLLIVDDNKVNRLLLARSLEQQGHSISFAENGRQA
                     LEILHAQPFDLVILDVLMPELDGYQVLEQMVSDQHLRDIPVIMASSSEELDMVVRCIE
                     IGAEDYLHKPVNPVLLKARINASLEKKRLRDQQRDLIRKFATEEVADDLMQTGFELGG
                     KYVEATVMFSDIRSFTTITESQTPSETIELLNTYYALMFDAIGGHGGVVNQMIGDGLM
                     CVFGAPLPQANHREQAVRAALEMVALIDQFNQYQAAENKQQIKIGVGVATGQVIAGYT
                     GTQLRATYTCVGDTVNLAARLESHTKVVGQSIVIDETTRAGLNPDIEVDDQGMVQVKG
                     KTIEVHIYSVKAAQASLPAA"
     gene            3310..4551
                     /locus_tag="VFF70_03980"
     CDS             3310..4551
                     /locus_tag="VFF70_03980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZY43887.1"
                     /translation="MTEELISRVPLFASLPKHEIEHLAKSLRPFEFPIDTIIFHEGRK
                     EDHFYILIDGQVEILKALGTPDERLLGVREAVSFIGEMSLFSADGKHTASVRALSPLH
                     LLEMTREDFDALLHRQPKLAYEMVRVLSTRLQTSEDLTIHDLQAKNEQLTIAYNELRA
                     AHEQIVEKEKLERELAVAREIQQSILPRELPVVKGFDFGGKMMPTRAVAGDFFDVIWL
                     GPDQIGLVVGDVSDKGVPASLFMALTYSLIRAEASRAAASPRNALMMVNRHLLGMNAS
                     GMFVTVLYGILNCATLEFHYVRAGHTMPMVLDGQSREVPLPIDLGQPLGMLPEPIIDE
                     QRITLSRGSTLLIYTDGVTEAFDPQRQMFGEERLSQTLRSLDDQSAQTICNQLAEKVV
                     QYTQPLMPHDDITLVTVKVQR"
     gene            4548..5246
                     /locus_tag="VFF70_03985"
     CDS             4548..5246
                     /locus_tag="VFF70_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZY43888.1"
                     /translation="MKESRRSVPEVIAFLRSVPLFADLDQATLNRLTRVCRSASLPKG
                     YMLFNYSDPAESAYVVYSGRIDIMLTTADGRELVINEMGPGDCFGELSLVTNLPRSTN
                     AVARRASDVLIIPRDEFLSELEANPKMLRCLLETTAHRLQTSGEREGALAFLDAAARL
                     ARVLLQLDLEFSADGFVTISQDDLAQRLGLARQTVAKTLGQWRRSGWLVTGRGKIVVL
                     NRPALRRIAEEADY"
     gene            5393..>5770
                     /locus_tag="VFF70_03990"
     CDS             5393..>5770
                     /locus_tag="VFF70_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43889.1"
                     /translation="MAKTRNTQATKRLIKKLSAVRQTLSKSERDVLDKLVTGEVKGHS
                     MTRRKSVGAKTAAKNEVMGHSMTRSKSVGAKTAAKNEVMGHSMTRSKSVGAKTAAKNE
                     VMGHSMTRSKSVGAKTAAKNEVMG"
BASE COUNT         1361 a         1497 c         1709 g         1203 t
ORIGIN      
        1 cttttgagaa aggactggat cttgtataca cgatcgatga tcatgtgccg ccggttattc
       61 aaggcgatgt cacgcgtcta cggcagatca ttctcaacct gttgtcaaat gcgatcaagt
      121 ttaccgggaa gggtgaagtg atattggaag tgatggaaga tcgaagccgt gaagcggccg
      181 gtggacaatc gggccggggt agttcgggct cgttggactt cggtctgcac ttctcggtaa
      241 aagattccgg actcggcatt ccggccgatc ggatgaaccg gctcttccag tcattcagcc
      301 aagtggatgc ctcaaccgcg agaaaatacg ggggcacggg tctgggtctg gcgattagca
      361 aacggctgag cgaattgatg ggtggcagca tgtgggcgga gagtgaaggc attcccggtc
      421 atggcgccac gttccatttc aacattcaaa cccgcgcggc caaagcgccg gaacagacgc
      481 ggcgtgatct gcgcggtgcg cagccgcagt tgaaggataa gcgcgtgttg attgtcgacg
      541 acaacgacac caatcgacgc atcatttcac tgcaattgca gaagtggggc atgctcacgc
      601 gcgacaccgc ctcgccgcgc gaagcgttga aatggctgga gcacggcgat ccgtttgatg
      661 tggccattct ggatatgcac atgccggaga tggacggtat agaattagcg aaagagattc
      721 gcaagcagcg tgacgcaaaa aaacttccgc tggcgctgtt tacctcattg ggccgccgcg
      781 aagcggacgc tgaatcgatc gggtttgcgg cccatctcaa taagccgttg aaaccgtcca
      841 cgttgttcga tgcgatgatt ggcattttct ccgccgaagg cactccgcgg gcagcgcccg
      901 ccgttagcgc caagccgcag ttagatccga acatggccca gcgtttgccc ttgcggattt
      961 tgttagcgga agataacgcc gtgaatcaga aactggcgct caggttgttg gaacaaatgg
     1021 ggtatcgcgc agatgtggcg ggcaatggcc tcgaagcgat cgaagcggtc gagcggcaga
     1081 cgtatgatgt gatcttgatg gacgttcaga tgccggagtt ggacggactg gatgcaacgc
     1141 gcgagattcg caaattgaag gttgccgcgc agccgcgcat cgtggcgatg accgccaacg
     1201 ccatgcaggg cgatcgcgag atgtgcctgg cggccggtat ggatgattac atcagcaagc
     1261 cgatccatat tgaagagttg gtcagcgctt tgcagcagac caagcgctca atggccgaag
     1321 tgcaaggcca taacggacgg ccccagcagt tgtaatcgga gattattcac gttccacact
     1381 ctgcttgact ggtgcaacgc ttatcctatg tgctgcacaa cgtgcggtca cagatcgcgt
     1441 gtgagcaaac actttattaa tattgaatca ctcacatggc taagctgaaa ctcgatctgc
     1501 atgacatcta taatcgcggt gatcagattg acggtgaact tgatcggatt atccacgaag
     1561 cgatcgatcg caagatcgat ctcgtcgaga ttattccagg caagggcagc gggcagttga
     1621 aaaagaaagt gctgcgcttt ctaaatcaaa gccacatccg gcagttatat catcgggtgg
     1681 agaaggacga taaaaacttc gggcgcatct tcatccactt tcggcattaa cgcgcaaaca
     1741 atttgaaaca acaatggtat cattaggacc tacccccagg agtgacagtg tcaaggagga
     1801 tgtatggcag acccggtgat tgaccccacc acctttcaaa gcttgattgc gggcacggac
     1861 tcggcctttg tcatggaact gatcgataca ttcctggacg atgcgccgac gatgctgaac
     1921 gatctgcggc aatctttggc ggagaaaaat gcggaagtgt ttcggcgcgc agcgcattcg
     1981 ttaaaatcca acagcgccag cttcggtgcg ctgcatttgt caacgcaagc gaaggaattg
     2041 gaactattgg gcaaggccgg cgacctcgca caggtgggcg ataaactcac cgctttaacc
     2101 accgagttcg atcgagtgca agtggcactc aggaactggc agcatggatc gaagtaaagg
     2161 ctataccggt cgattgttga tcgtcgacga caacaaagtc aatcgcctat tattggcgcg
     2221 cagcttggag cagcaaggtc actccatctc ttttgcggag aatgggcggc aagcgttgga
     2281 gatcctgcat gcccagccat ttgatctagt gatcctcgat gtgttgatgc cggagctgga
     2341 cgggtatcaa gtgctggagc agatggtgtc cgatcagcac ctgcgtgaca ttccggtgat
     2401 tatggcttcg tcgtcggaag agctggatat ggtagtgcgc tgcatcgaga tcggcgcgga
     2461 agattatttg cacaagccgg tcaacccggt gctgctcaaa gcgcgcatca atgccagcct
     2521 ggaaaagaag cgcctgcgcg atcagcagcg cgatctcatt cgcaagtttg cgactgaaga
     2581 agtcgccgat gatttgatgc agaccggctt cgagttaggc ggcaagtatg tcgaagcgac
     2641 cgtgatgttc tccgatattc gatcgtttac gacgatcacc gaatcgcaaa cgccgtcgga
     2701 aacgatcgag ctgctgaaca cgtattacgc gctgatgttc gatgcgatcg gcgggcatgg
     2761 tggcgtggtc aatcaaatga tcggcgatgg gctgatgtgc gtcttcggcg cgccgttgcc
     2821 gcaggccaat catcgtgagc aggccgtgcg cgccgcgttg gagatggtcg cgttgatcga
     2881 tcaatttaat caatatcaag ccgcggagaa taagcagcag atcaaaatcg gtgtgggtgt
     2941 ggcgacggga caagtgatcg ccggttacac cggtacgcag ttacgcgcca cctatacgtg
     3001 cgtcggcgat accgtcaatc tggccgcgcg tttggaatca catacgaaag tggtcggcca
     3061 gtcgattgtc attgatgaaa cgacacgcgc cgggttaaac cctgacatcg aagtggacga
     3121 tcaaggcatg gtgcaggtta agggcaagac catagaagtg catatctatt ccgtgaaggc
     3181 cgctcaagct agcctgccag ccgcatgaac gcttccatct cggttaaatt tcccgtaaat
     3241 gatcaagact ccgaaatctc cccaatccat tttgcagtct gctcagtgta aactcggccg
     3301 gagttgccaa tgactgaaga actaatcagc cgtgtgccgc tgttcgcttc actgcccaaa
     3361 cacgagatcg agcatctagc caaatcgctg cggccgtttg aattccccat cgataccatc
     3421 attttccacg aaggccgcaa agaagatcac ttctacattc tgatcgacgg acaagtcgag
     3481 attcttaaag cgctgggcac gccggatgaa cgcttgttgg gtgtgcgcga agcggtttcc
     3541 ttcattggcg aaatgagttt gtttagcgcg gatggcaaac atacggccag cgtgcgagcc
     3601 ctgtcgccgt tgcatctgct ggaaatgacg cgtgaagatt tcgatgcgct gctccatcgc
     3661 caaccgaagc tagcttacga aatggtgcgc gtgctcagca cccgcttgca gacctcggag
     3721 gatctcacga tccatgatct gcaagcgaag aacgagcagc tgacgatcgc ttacaacgaa
     3781 ctgcgcgctg cacacgagca gatcgtcgag aaggaaaaac tggaacgcga gttggcggtg
     3841 gcgcgtgaaa ttcagcagag cattctgccg cgcgaactgc ccgtcgtcaa aggttttgac
     3901 tttggcggga agatgatgcc cacccgcgct gtggccggag atttctttga cgtgatctgg
     3961 cttggcccgg atcaaatcgg gctggtcgtc ggcgatgtgt cagataaagg cgtgccggct
     4021 tcgctgttca tggccctgac ttacagtctc atccgcgcgg aagccagccg cgccgccgct
     4081 tcaccgcgca acgcgttaat gatggtcaat cgtcacttat tgggcatgaa tgcttcgggt
     4141 atgttcgtga ctgtcctgta tggcatcctg aactgtgcca cgctggaatt tcattacgtg
     4201 cgcgccggcc acacgatgcc gatggtgctg gatgggcaga gccgtgaagt gccactcccg
     4261 atcgatctgg gccagcccct cggtatgctg ccggaaccga tcatcgacga gcagcggatc
     4321 acattgtcgc gaggcagcac gctgttgatc tataccgacg gcgtgacaga agcgttcgat
     4381 ccgcagcgtc aaatgtttgg cgaagagcga ttgagccaga cactgcgcag cctagatgat
     4441 cagtcggcgc aaacgatctg caatcaactc gccgagaagg tggttcaata tacacagccg
     4501 ttaatgccgc atgacgacat tacgctggtg acggtgaaag tgcagcgatg aaagaatcgc
     4561 ggcgctccgt tccagaagtg attgcatttc tccgcagtgt gccactcttc gccgatctcg
     4621 atcaagcgac cctcaaccgc ttaacccgcg tctgtcgcag cgcctctctg cctaaaggct
     4681 acatgctgtt caattactcc gatccggccg agtccgcgta tgtcgtctac tccggccgca
     4741 tcgatattat gctgacgacg gcggatggtc gggagttggt catcaacgaa atggggccgg
     4801 gcgattgctt cggcgaactg tccttggtga cgaatttacc tcgatcgact aatgcggtgg
     4861 cgcgccgggc cagcgatgtc ctgatcattc cccgcgacga atttctgtcc gaattggaag
     4921 ccaatccgaa aatgctgcga tgcttattag aaacgacggc gcatcgattg cagaccagcg
     4981 gcgaacggga aggcgcgctg gcgtttttag atgcagcggc gcggttggcc cgggtcttgc
     5041 tgcagctcga tctggaattc agcgccgacg gctttgtgac catttcacag gatgatctcg
     5101 ctcagcggct gggactcgcc cggcagacgg tggctaaaac gttgggccaa tggcgccgat
     5161 cgggctggct ggtaacaggc cgcggcaaaa tcgtcgtgct taatcgcccg gcgctgcggc
     5221 gaatcgccga agaagcggat tattgaatcg aaaactcatg agccggtgtg tcacttgcgt
     5281 gacagacagg ccgatcgatt gaggctatac tttgcccaag agttgagaga aacagagcgg
     5341 tctcaatcaa aatctaaatc agaattctaa gatcccatag gagggacccc aaatggctaa
     5401 gacgcgaaac acccaggcaa cgaagcgatt gatcaagaag ttgtcggccg tgcgacagac
     5461 gctcagcaag agcgagcgcg acgttctgga taagctggtc accggcgaag tgaagggtca
     5521 ctcgatgacc cgcaggaagt cggttggtgc caagacggcc gcgaagaacg aagtgatggg
     5581 ccactcaatg acgcgcagca aatcagtcgg cgccaagacg gccgcgaaga acgaagtgat
     5641 gggccactcg atgacccgca gcaagtcggt cggcgcgaag acggccgcca agaacgaagt
     5701 gatgggtcac tcgatgaccc gcagcaagtc ggtcggcgcc aagacggccg cgaagaacga
     5761 agtgatgggc
//
LOCUS       DASRTJ010000188         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_193809,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000188 DASRTJ010000000
VERSION     DASRTJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193809"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1211)
                     /locus_tag="VFF70_03995"
     CDS             complement(147..1211)
                     /locus_tag="VFF70_03995"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HZY43890.1"
                     /translation="MNDLNDVTAIHAADPHGLHQHILNLPKQMEDAWAATDRVELPQS
                     LSQIDRVVIIGMGGSAIGGSLLSAVMSPESQLPVLVSRDYDLPAFARGSNALVIASSH
                     SGNTEETLSAFDQARSRGCHLIVLAAGGTLADRAREFHLPLITIDYQSQPRAALGWSF
                     APLLNIASRLKWTHDLKNDLDEAIAVLRSGNAQFSAESPVMKNLAKREAGQLMGRIVF
                     VFGAGYFAEVARRWKDQFNENAKHWAAFESLPEADHNLLAGLEFPDKFSNRVMALFLT
                     GSRDHPRNAKRIDLTRKEFMMAGCNTDILTPRGESPLAQMLSLIQLGDFMSFYLALLN
                     GAEPTQIDVMVEFKVRMADG"
     gene            complement(1258..2067)
                     /gene="rsmI"
                     /locus_tag="VFF70_04000"
     CDS             complement(1258..2067)
                     /gene="rsmI"
                     /locus_tag="VFF70_04000"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZY43891.1"
                     /translation="MGNLYLVPTPIGNLEDITLRALKVLRESTLIAAEDTRRAAFLLS
                     HFDLHTPVTSYFEHNKLSKLDRILSALDQGDVALISEAGTPGLSDPGYELIRAAIDHG
                     HTIVPLPGATAAITALVASGLPTDSFVFSGFLPRKAGERRRALAEVKNDRRTLIFYEA
                     PHRLKDALPDIGSILGDRSICVARELTKLHEEIWRGSVSGAIEHFSGEVLGEIVLVVA
                     GSIEAEIWDVDQVRSAVRDELDSGTSASETAKKIAALSGWNKREVYALINE"
     gene            complement(2100..2837)
                     /locus_tag="VFF70_04005"
     CDS             complement(2100..2837)
                     /locus_tag="VFF70_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZY43892.1"
                     /translation="MERKNRVATGCAGLDEMLAGGFIPGSANMVRGAPGTGKTLMGLQ
                     FLVHGALHKEPGLLISFEEFPQSLHTDAESLGWNLHALEEQGLLHLYFTSPQVLLQSL
                     SSPASSLSDLFREGGIQRVVLDSVTHFTRVTNDPQQLRSIYYSLVNALKREGVTSLLI
                     AEEGRADVQRSERSQLSFVVDTIILLRFVEIESAIQRAIAVLKMRGSDHAQEIRHVEI
                     QRGGLNVFGVFEGRESILTGISHRVSR"
     gene            complement(2904..>4246)
                     /locus_tag="VFF70_04010"
     CDS             complement(2904..>4246)
                     /locus_tag="VFF70_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43893.1"
                     /translation="SGTTIEVESLHRYFRNKLYPKLGEAYGKNFIAVTDPGTPLAALA
                     QKENFRRAFINQPDIGGRYSVLSYFGLVPMALSGVDIDRLLDRARAMARACGAAVSAK
                     DNPGVWLGAIMGELAQAGRDKITFILSKEVAAFGYWVEQLIAESTGKLGTGIVPIAGE
                     ALGSPKVYGADRLFVHLRLKTDRSLNPKVKALAKAGQPIVYLTLDDRYDLGAEFFRWE
                     MATVVAGAVLQLDPLDQPNVTESKNNTSAILKQYISTGKLLDNDSVGTSGRSPRQDAL
                     ASLLKQVKPGDYVALMAYVPMTPAIDRALQAIRGSIRDQLKVATTIGYGPRFLHSTGQ
                     LHKGGANKGVFIQLTADAAKDLKIEGKEFSFGTLIRAQALGDLQALRQRNYRVVRIHL
                     GQDVADGLRSVQSAIEQALGAKKQASTHRAAKKSIKRPAAKRSTAQRSGQKSKR"
BASE COUNT          929 a         1265 c         1120 g          932 t
ORIGIN      
        1 gaatagcgtg tgtgtcactc atggcagcct ctatttggtc caatcaattg gttcgactaa
       61 aagtatagca catcgacgat attcttgaaa caagcagccc gattcgaaat tcgatcgggc
      121 ttttatttgc gatatgcgat aagccatcag ccatccgcca tccgcacctt gaactccacc
      181 atcacatcga tctgcgtcgg ctctgcgcca ttcaacagcg ctagatagaa actcatgaag
      241 tcgcccaact gaatcagcga cagcatctgc gccaacggcg attcgccgcg cggggtcaag
      301 atgtccgtat tgcagcccgc catcataaat tctttgcgcg tcagatcgat ccgcttcgcg
      361 ttgcgcggat ggtcgcgcga gccagtgagg aacaacgcca tcactctatt cgagaacttg
      421 tcaggaaatt ccaggcctgc cagcaaattg tgatcggctt cgggcagtga ttcaaacgcc
      481 gcccaatgct tcgcgttctc attgaattga tctttccagc ggcgggccac ttcggcaaaa
      541 tagcctgcgc caaacacaaa cacgatccgt cccatcaact gcccggcttc gcgcttggct
      601 aaatttttca tcaccggcga ttcggcgctg aactgcgcgt tgccgctgcg caggacagcg
      661 atcgcttcgt ccagatcgtt tttgagatcg tgcgtccatt tcaaacgcga ggcgatattc
      721 agcagcggcg cgaacgacca gcctaaggct gcgcgcggct gcgattgata atcgatcgtg
      781 atcaggggca ggtgaaattc ccgcgcccga tcggccaacg tgcccccggc ggctaatacg
      841 atcaagtgac agccgcgcga tcgagcctga tcgaacgcgg ataaagtttc ttcggtgttg
      901 cccgaatgcg acgaagcaat caccagcgca ttcgatccgc gggcgaatgc gggtaaatcg
      961 taatcgcggg aaacgaggac aggcaattgg ctttcaggtg acatcacagc agacagcagc
     1021 gatccaccga tcgccgagcc gcccatgccg atgatcacaa cgcgatcaat ctggctcaaa
     1081 ctctgcggca attcaacacg atccgtcgcg gcccaggcat cttccatttg tttcggcaga
     1141 ttcaggatgt gctggtgcag accgtgagga tcagctgcgt gaatggctgt tacatcgttg
     1201 agatcattca tgacggctcc aagaaaaatt cttaatgctc aacgcacctg gatgctttca
     1261 ttcgtttatt aacgcataaa cttcgcgctt gttccagccc gacagcgcgg cgatcttttt
     1321 cgcggtctca ctcgccgaag tgccggagtc caattcgtcg cgtacagctg atcggacttg
     1381 atcaacatcc caaatttcgg cttcgatcga tccagcgacg actaacacaa tctcacctaa
     1441 tacttcgccg ctgaaatgct cgatcgcgcc gctgaccgag ccgcgccaaa tttcttcatg
     1501 cagcttcgtg agttcacgcg ccacgcagat cgatcgatcg cccaggatcg atccgatgtc
     1561 tggcaatgca tctttcaagc gatgcggcgc ttcatagaaa atcagcgtgc gccgatcgtt
     1621 cttcacttca gccaacgctc gccgccgctc accggccttg cgcggcagga aaccgctaaa
     1681 gacaaatgaa tcggtcggca gaccggaagc caccaacgcg gtgatggccg ccgtcgcgcc
     1741 gggcagcggc acgatcgtgt ggccgtgatc gatcgccgcg cggatcaatt cgtagcccgg
     1801 atcggataaa cccggcgtgc ccgcttcgga gatcaacgcg acatcgcctt gatcgagcgc
     1861 gctcaaaatg cgatcaagct tgctcagttt gttgtgctcg aagtaggaag tgaccggcgt
     1921 gtgcagatcg aagtgtgaca acaagaacgc cgcgcggcgg gtgtcttcgg cggcgatcag
     1981 tgttgattcg cgcaaaactt tcagcgcgcg caaggtgatg tcttccaaat taccgattgg
     2041 agttggaacg aggtagaggt tacccatgtg gggatcggtg gggcgaggtg accttgcccc
     2101 tagcgtgaca ctcgatgcga aattccagtg agtatacttt cgcggccttc gaatacgccg
     2161 aacacattca agccgccgcg ttgaatttcc acatgccgaa tttcctgagc gtgatcgctg
     2221 ccgcgcattt ttagcacggc aatcgcacgt tgaatggcgc tctcgatctc gacaaagcgc
     2281 agcaggataa tcgtgtcgac gacaaacgac aactggctgc gctccgagcg ctgcacatcg
     2341 gcgcggccct cctcggcgat caacaatgaa gtgacccctt cacgtttgag cgcattcacc
     2401 agcgaatagt agatgctgcg cagctgttgt ggatcgttcg tcacacgcgt aaagtgcgtg
     2461 acgctatcca gcaccacacg ctggatgccg ccctcacgga acaggtcgct taatgaactc
     2521 gccggcgagg acaaactttg cagcaagact tgcggcgagg taaaatataa atgcagcagc
     2581 ccctgctctt ccaaggcatg cagattccag cccaaggact cggcatccgt gtgcagcgat
     2641 tgcggaaatt cttcaaacga aatcaacagg cccggctctt tatgcagcgc accatgcacc
     2701 aggaattgca gacccatcaa cgtcttgccc gtgcctggcg cgccgcgcac catgttcgcc
     2761 gatccgggaa tgaacccgcc agccaacatt tcatccaaac ccgcacagcc cgttgcgacc
     2821 cgattttttc gttccatgtt tattagtccc ggcgtgaaat caagttgatc atagcacgcc
     2881 tcagcttctc aagcaaactg gttttaacgc ttcgatttct ggcctgatcg ttgcgctgtc
     2941 gatcgttttg cggccggtcg cttgatcgat ttctttgcgg ctcggtgtgt gctcgcttgt
     3001 tttttcgcgc ccaacgcttg ctcgatcgcg ctctgcaccg atcgcaagcc gtcggcgaca
     3061 tcctggccca gatgaatccg cacgacccgg tagttgcgct gacgcagcgc ttgcaagtcg
     3121 ccgagtgctt gcgcgcggat caaggtgccg aaagaaaact ctttgccttc gatcttgaga
     3181 tctttcgcgg catcggcggt caattgaatg aacacgccct tattggctcc gcctttgtgc
     3241 agctgtccgg tcgaatgcaa gaagcgcggc ccgtagccga tcgtcgtcgc caccttcaat
     3301 tgatcgcgga tcgatccacg gatcgcctgc aacgcccgat cgatcgccgg ggtcatgggg
     3361 acatacgcca tcagcgccac gtaatcgccg ggcttaactt gttttaataa tgaagcgagc
     3421 gcatcttgtc ggggcgagcg gccgctcgtc ccgacagaat cattatccag caacttgccg
     3481 gttgaaatat actgtttcaa gatcgcgctg gtattgttct tgctttcagt cacattcggc
     3541 tgatcgagcg gatcgagttg caggactgcg cccgcaacga ccgtggccat ttcccagcga
     3601 aagaactccg cgccgagatc atagcgatcg tcgagcgtga gataaacaat cggttgaccg
     3661 gctttcgcta aagctttcac tttcggattg agtgatcgat cggtcttcaa acgcagatgc
     3721 acaaacaatc gatccgcgcc gtaaactttc ggcgagccca gtgcttcacc tgcgatcggt
     3781 acgatgcctg tgccgagctt gccggtcgat tcggcgatca actgctcgac ccaatagccg
     3841 aacgcggcca cttcttttga cagaatgaag gtgatcttgt cgcggccagc ctgtgcgagt
     3901 tcgcccatga tcgcgcccaa ccacacgccc ggattatctt tcgctgaaac ggctgcgccg
     3961 cacgcgcgcg ccatcgcccg cgcccgatcg agcaagcgat cgatatcgac gccgctcaac
     4021 gccatcggca ctaacccaaa atacgacaac accgaatagc gcccgccgat gtctggttga
     4081 ttgataaacg cgcgccggaa attttctttc tgggccaacg ccgccagcgg tgtgcccgga
     4141 tcggtcaccg cgatgaaatt ttttccgtat gcctcgccga gtttcggata cagcttgttt
     4201 ctgaaatagc ggtgcagcga ttccacttcg atcgtcgtgc cggact
//
LOCUS       DASRTJ010000189         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_10716,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000189 DASRTJ010000000
VERSION     DASRTJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10716"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFF70_04015"
     CDS             complement(<1..386)
                     /locus_tag="VFF70_04015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955047.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZY43894.1"
                     /translation="MRNKISIIGAGNVGASCALWLAQANVADLVLVDIPQAETMPVGK
                     SLDLLQAGPIIGYDSRITGTANGSYEGTENSDIIIITGGLPRKPGMSREDLVGANQQV
                     VTNIIDNALPKSPNAIVIVVTNPLDT"
     gene            complement(523..1518)
                     /locus_tag="VFF70_04020"
     CDS             complement(523..1518)
                     /locus_tag="VFF70_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZY43895.1"
                     /translation="MTTSTAQPRFNYGGQAVIEGVMMRGSKALAVAVRDPHGQIVMHS
                     EPVNAALYSGSISRIPFLRGLSMLWDALGLGMRSLMYSANIAASAIDQSPAASPVNPI
                     DPAGAIESAPTDLKTNDIFNGPLGWGVMAISLAFALAIFLFLPKWIASWFEGSVTSLA
                     SAFIEGVVRLLLFIGYISLIGRSKEIGRVFGYHGAEHKTINAYEAGDTLTVENVQRHS
                     LQHPRCGTAFLLTVVLLSIIFFAPLNGLPFGLGLILRVVLLPVVAMVAYEYIRFSAKH
                     LKNPIMRALIAPNLAMQLLTTRQPDNSMVEVAITALKKVLDSEQVKPLNPEQMIP"
     gene            1661..>2383
                     /locus_tag="VFF70_04025"
     CDS             1661..>2383
                     /locus_tag="VFF70_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZY43896.1"
                     /translation="MKRPLALLISLVLILTACAPAATTLPTSAPPAARPTTAPAAPAP
                     AQPIATANATSGSTDLRPPVSGGLTNPPTISPKQPDTQPNSSNRVKGWQQLGSPIVPP
                     ARYDHVLVFAGDFNRLVLFGGRAAATLNDTWVYDLAANAWHAVTGSIAPEARYGAGAA
                     YDFARKHVVLFGGQSAAKFFSDVWIFDIETEKWSKLTTIGTAPTARSSMAAAIDRPYD
                     QLIISQGATAQGRLDDTWSLALA"
BASE COUNT          534 a          698 c          653 g          498 t
ORIGIN      
        1 atcgtgtcga gcgggttggt cacgacgatg acgatcgcgt tcggcgattt gggcagcgcg
       61 ttatcgataa tgttcgtgac cacctgctga ttcgcaccga cgagatcttc gcggctcatg
      121 cccggcttac gcggcaggcc gccggtgata ataatgatat ctgagttctc agtgccttcg
      181 tacgagccat tggccgtgcc cgtgatgcgc gaatcatagc cgatgatcgg tccggcttgc
      241 agcagatcga gcgacttgcc gaccggcatg gtctcggctt gcggaatatc gaccaagacc
      301 aaatccgcga cattcgcttg cgccagccac aacgcgcacg atgcgccgac gttgcccgcg
      361 ccgataatac tgattttgtt tcgcatggtt taaatctcct tgagaaagat aattgttgca
      421 gagacgcaac ggtgaagcat ctgagacgtt ccgctggaac gtctctacgt taagagacaa
      481 caatcgggct tgagatgaga ttcacctcaa gcccgatatc agttatggga tcatctgttc
      541 cggattcaac ggcttcactt gctcggagtc caaaactttt ttgagcgccg taatcgccac
      601 ttccaccatc gagttgtccg gctggcgagt ggtgagcaac tgcatcgcca gatttggcgc
      661 gattaaggca cgcatgatcg gatttttgag atgcttggcc gagaagcgaa tgtattcgta
      721 agcgaccatg gcgacgaccg gcaacagcac aacccgcaaa atcaggccca gcccgaacgg
      781 caggccattc aagggagcaa agaaaatgat cgagagcaga accaccgtta agaggaaggc
      841 cgtgccgcag cgcggatgct gcagcgagtg ccgctgcaca ttctcgacgg tgagagtgtc
      901 gcccgcttca tacgcattga tcgttttgtg ttccgcgccg tgatagccga acacgcggcc
      961 aatttctttc gaacgaccga tcaagctaat gtagccgatg aacagcagca gccgcaccac
     1021 gccttcgatg aacgcgctgg cgagtgaggt gaccgatcct tcaaaccaag acgcaatcca
     1081 tttgggcagg aacaaaaaaa tcgctaacgc aaaagccagc gagatcgcca ttacgcccca
     1141 acccagcggg ccgttgaaaa tatcattggt tttcaggtcg gtcggcgcgg actcgatcgc
     1201 gccagctggg tcaatcgggt ttaccgggct cgccgctggc gattgatcga tcgcactcgc
     1261 cgctatgttc gcggagtaca tcaagctgcg catgcccaag cccagcgcat cccacaacat
     1321 cgacagaccg cgcagaaagg gaatgcgcga gatcgatccg ctatataacg cggcattgac
     1381 cggttcagag tgcatcacga tctggccgtg gggatcgcgc actgcaacgg ctaaggcttt
     1441 cgagccgcgc atcattacgc cttcgatgac tgcctgcccg ccgtaattaa atcttggttg
     1501 tgctgttgaa gttgtcatgc agcgattcta ccctgcgcag ctagtcgtgt caagaatcgt
     1561 tcagacaaaa taaacccgat gtcttaaaga tgaaactttt ccagcccggc ctgcgtcacg
     1621 aatctagatg tcagatatat aatgaaggcg gaggcctatt atgaagcgcc cactcgctct
     1681 cttgattagc ctggtgctta tcctaaccgc ttgcgcgccc gcggcaacaa cgctgccaac
     1741 atccgcgccg ccggctgctc gacccaccac tgcgcccgcc gccccggctc cggctcaacc
     1801 gatcgcaact gccaatgcaa ccagcggctc aaccgatctg cgtccgcctg tttccggcgg
     1861 cctgacgaat ccgccgacga tctcgcccaa gcaaccggac acgcaaccga attcatcgaa
     1921 tcgagtcaaa ggctggcagc aattgggttc accgattgtt ccaccggctc gatacgatca
     1981 cgtcctcgtc tttgccggcg atttcaatcg attggttttg tttggcggcc gagcggctgc
     2041 cacgctcaac gacacctggg tctacgatct agcagcaaac gcgtggcatg ccgtcaccgg
     2101 atcgatcgcg ccggaggcgc gctatggcgc aggcgcagct tacgatttcg cccgcaagca
     2161 tgttgtactc ttcggcggac aatcggctgc aaaatttttc agcgatgtgt ggattttcga
     2221 tattgaaacg gagaagtgga gcaagctcac gacgatcggc actgcgccca ctgcccgatc
     2281 gagtatggcc gcggcgatcg atcggccata cgatcagttg atcatctcgc agggtgcaac
     2341 ggctcagggc cgcctggatg atacgtggtc tttggcactc gcc
//
LOCUS       DASRTJ010000190         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_237884,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000190 DASRTJ010000000
VERSION     DASRTJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237884"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFF70_04030"
     CDS             complement(<1..706)
                     /locus_tag="VFF70_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY43897.1"
                     /translation="MASSIDQSVVCPILIGRASNLDAIDRLIDQAASGAGRVALISGE
                     AGLGKSRLVSEAKSIAALHGLLTLQGGCFEIDRSLPYAPFIDLLRSFFANFSTDQIKR
                     FVDPAALDLSRLLPEFSATHNEVSSAIDPEQEKRRLFQALSQFLIQLAATQPLLVVIE
                     DLHWCDDTSLELLAFLARRLNSQPILLLLTYRNDEIDARVNHLLAELDREHIATELVL
                     KRFAQAEVDAMLRAIFG"
     gene            1070..1540
                     /locus_tag="VFF70_04035"
     CDS             1070..1540
                     /locus_tag="VFF70_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZY43898.1"
                     /translation="MDTIQLIIGAVVWLAITFGAAFLGTRYLPDEWYKQIRKPTWNPP
                     NKIFAPVWSVLYLLMAIAAWLVWKHNDLTETILPLTLFIVQLLLNAAWTWLFFGRHRL
                     DTALMDIVVLWLVILTVIILFWGVEPLAGILLVPYLAWVSFATFLNYTILRLNR"
     gene            complement(1628..>3038)
                     /locus_tag="VFF70_04040"
     CDS             complement(1628..>3038)
                     /locus_tag="VFF70_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HZY43899.1"
                     /translation="GGSYNDGGAFVFSVKDETLSCTDKFGRFINVTEQPQRRSESVEI
                     TSQSKDASQSDGAKASWSDRLKAEDVLKLFDSITLGNRHPALAHWSMNEIDALVNDLV
                     ALAQTDASRRRAVIDLLTVLHDRRYDTGANKRRLIIAHLNVALDAVFRSVTPISHEDR
                     SSADRLIRWENRIAPIPGPFPVIDKGREQTPPLRSGGGREGVLFIDCTGFEPQGADSV
                     GRLIVKANQLGWREFIVFACRGDRFIGCGLGANSDDVRIDVYGSSGDYLGSGLDGGEI
                     QVHGDAQDQVGQILKRGKLVIHGMVGQTFLYGAKGGVAYVMDNAAGRPLINAVGSIRT
                     IINGTALDYAAESFMAGATTGGGFLIINGVSFNDKGSIVPLIDRYPGGNFFSLASGGA
                     GYINDPDWTLGYDQLNGGEIVGFEEADWNVIEPYLKENEKLFGIKLSDLLNGHEPNRA
                     YRKVVPERSGEVKNLDAHT"
BASE COUNT          727 a          850 c          754 g          707 t
ORIGIN      
        1 acccaaagat ggcgcgcagc atcgcatcga cttcagcctg agcgaaccgc ttcaagacga
       61 gttcggtagc aatgtgttcc cgatcgagtt cggctaacaa atggttcact cgcgcgtcga
      121 tctcgtcgtt gcgataggta agcagtaaaa gtatcggctg tgaattcaaa cggcgcgcca
      181 ggaaagccaa taattcgagg ctggtgtcat cgcaccaatg caagtcttca atgacgacca
      241 aaagtggctg agtggcagcc agttgaatca gaaattgcga cagggcttga aacagccgcc
      301 gcttttcttg ttcgggatca attgcactcg agacttcgtt gtgcgtggca gagaattcag
      361 gcagcagccg cgagagatcg agcgcagccg gatcaacgaa gcgtttgatc tgatcggtgg
      421 aaaagttggc gaagaaactc cgcagtagat cgatgaacgg cgcatagggc agcgatcgat
      481 cgatctcgaa gcagccgcct tgcagcgtca acaggccgtg cagtgctgcg atcgatttcg
      541 cttccgacac caatcgggat ttgccgagac ccgcttcgcc ggagatgagt gcgacacggc
      601 ctgcgccgct ggcagcttga tcgatgagcc gatcgatcgc atcgaggttc gacgcgcggc
      661 caatgaggat gggacaaact acagactgat caattgaaga tgccattctt tcccttggca
      721 aaatcgatca cgatcgatcc aacactgaat ggcgatgaag acgcagaaga gggcttgatt
      781 atatacttag tgggttgaac ttccaagcag gcgagggaag ttgtaactca aacgaaacac
      841 cttcgcgctg acaagctggc ccgattgaca atcgagacga atgacctaaa cttattggca
      901 gacgaattta aatacattcg caagagcact gctgaataga gtcattcccg cgcaggcggg
      961 aatccagcag aacactggat acccgcctcc gcgggtgtgt cagctacgac aagtattcgg
     1021 ccaaattatc caggttgcat ttagtgagag gttgtgaagg agtcggtcga tggacactat
     1081 tcaattgatt atcggcgcgg tggtgtggtt agcgattact ttcggagctg cgttcctcgg
     1141 cactcgatat ttgccagacg agtggtataa acaaatccgc aagccgactt ggaacccgcc
     1201 gaataaaatc tttgcgccgg tatggtctgt gctctatctg ctgatggcga tcgcagcctg
     1261 gttggtgtgg aaacacaacg acctcaccga aacgatcctg ccgctcacgt tgttcatcgt
     1321 tcaactcctg ctgaatgctg catggacatg gttgttcttc ggtcgacatc gactcgacac
     1381 ggcgctgatg gacatcgtcg tgttgtggtt ggtaatcctc accgtgatca ttctcttctg
     1441 gggagtggag ccgctcgccg gtattctgct ggtgccttac ctcgcatggg tttcgttcgc
     1501 aacatttctc aactacacga tcttgcgctt gaaccggtaa agttagcgcg ataatgtcat
     1561 cctacagaga aaaggccaac tcatcttcga gttggccttt cacgtatcac gcatcactct
     1621 ttacatttca cgtatgcgcg tcgaggttct ttacttcacc cgatcgctcc ggcacgactt
     1681 tgcggtaggc gcgatttggt tcatgcccat tcagcaagtc actcaatttt atgccgaaca
     1741 atttttcatt ctctttcaag tacggctcga tcacgttcca atcggcctct tcaaatccga
     1801 cgatttcgcc gccattcaat tgatcgtaac ccagcgtcca gtccggatcg ttgatgtagc
     1861 ctgcgccgcc cgaagccagt gagaagaaat tgccgccggg ataccgatcg atcagcggga
     1921 cgattgatcc tttatcgttg aatgaaacgc cgttgatgat caagaatccg ccgcctgtcg
     1981 tcgcgccggc catgaatgat tcagccgcat aatcaagcgc cgtgccatta atgatcgtgc
     2041 ggatcgatcc gaccgcgttg atcaacggtc ggccggccgc gttgtccatc acatacgcga
     2101 caccgccttt cgcgccgtac aaaaatgtct ggccgaccat gccgtgaatc accaatttgc
     2161 ctcgcttcaa aatctgcccg acttgatctt gcgcatcgcc gtgcacctga atctcgccgc
     2221 catccaaacc cgagccaaga taatcgccgc tgctgccata cacatcgatg cggacgtcat
     2281 cgctgtttgc gcccaatcca cagccgatga atcgatcgcc gcgacaggcg aacacaataa
     2341 attctctcca acccaattga ttggccttca cgatcaatcg ccctacggaa tctgcacctt
     2401 gtggttcaaa gccagtgcag tcaatgaaga gaaccccctc ccggcctccc ccgctgcgta
     2461 ggggaggagt ctgctccctt cccttgtcga tgacggggaa aggaccgggg atgggggcaa
     2521 ttcgattctc ccaccgaatc aatcgatcag cgctcgatcg atcctcatgg ctgatcggcg
     2581 ttaccgatcg gaatactgcg tccaacgcga cattcaagtg agcaataatc agcctgcgtt
     2641 tattcgcgcc cgtgtcatag cgtcgatcat gaagcactgt caacaaatca atcacggcgc
     2701 ggcgcctact tgcatcagtc tgagccagcg ctaccaaatc atttaccagc gcgtcgatct
     2761 cattcatcga ccagtgtgct aatgcgggat gccgattacc taatgtaatt gaatcaaaca
     2821 atttcaacac atcttcagct ttaagccgat cactccaact cgccttcgcg ccgtctgact
     2881 gactcgcgtc cttcgactgc gacgtgattt caacggactc acttcttcgc tgaggctgct
     2941 cagtaacatt tatgaagcgc ccaaacttat cagtgcaact caacgtttca tctttaaccg
     3001 aaaacacaaa cgcgccacca tcattgtaac ttccaccc
//
LOCUS       DASRTJ010000191         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_345201,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000191 DASRTJ010000000
VERSION     DASRTJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345201"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="holB"
                     /locus_tag="VFF70_04045"
     CDS             <1..948
                     /gene="holB"
                     /locus_tag="VFF70_04045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZY43900.1"
                     /translation="NLDRSVAEHRAAQAYLISGPAHIGKTTVARALALALNCESKTDR
                     PDLHCRSCQLIQNDRHPDVQVIEPDGAHLKIEQVRALQHDLALSPVEGKFRVSIFDQF
                     ELATIEAQNALLKTLEEPPSYAVLIVLASDPELLLPTIVSRCQLITLRPLTMAQVRAA
                     LIDQWKVNNDQANLLAHLSGGRLGWAINAVTDRSIMENRSKLLDDLVQLLKSDRVARF
                     AYAEGITRKDGRTREAIEVWQTWWRDVMLAASQSTAEPINIDRAAELVTMSKQIDLDR
                     AHAAVEACAKATWQLERNAAARQVMEILLLDFPKVKSEK"
     gene            complement(1083..1925)
                     /locus_tag="VFF70_04050"
     CDS             complement(1083..1925)
                     /locus_tag="VFF70_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZY43901.1"
                     /translation="MTNHVDLHMHSTASDGTLRPSEVVQLAKSRGLTTIALTDHDTTD
                     GLAEAIDAGRSIGLAVIVGLEINSEDDQTDTHFLAYGLDRSNVPLQDQLLAIRDARVG
                     RAKGMLKKLAAMGMPVNWDRVMKYAGDAQSIARPHIARAMVEAGHVASMQEAFDKYIS
                     DDGPAYVHRLKLTPQEVIDLVHGAGGAIVLAHPAHAQTTPLVTQFVELGLDGVEVYYS
                     DHTPELRAELLSLAQQYDLIVTGGSDFHSPGDKSHAELGSIFVPDEVVQRLKERIERQ
                     RTIQ"
     gene            complement(1936..2151)
                     /locus_tag="VFF70_04055"
     CDS             complement(1936..2151)
                     /locus_tag="VFF70_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43902.1"
                     /translation="MRAKSYEALPGRSGAVIAVTSIIGGINLFVPLILGGIADAIGLQ
                     SAMWLLMIGPVALVLGLREKRSVDRVW"
BASE COUNT          517 a          642 c          553 g          452 t
ORIGIN      
        1 aatctcgatc gatcggtggc cgaacaccgc gcggctcaag cttatttaat cagcggccca
       61 gcccacattg gcaagacgac ggtggcccgc gcactggcac ttgctttaaa ctgtgaatca
      121 aaaacggatc ggcccgatct acattgtcga tcttgccaac tcattcaaaa cgatcggcat
      181 cccgatgtgc aggtgatcga accagacgga gcacacctca agatcgagca agtccgcgca
      241 ctgcaacatg atctggcact cagccccgtc gaaggcaaat tccgcgtgtc catcttcgat
      301 caatttgaat tggcgacaat cgaagcccag aatgcgctgc ttaaaacgtt ggaagagcct
      361 ccaagttatg cggtgctgat cgttttagcg tccgatccgg aactcttgct gccgacgatc
      421 gtctcgcgct gtcaattgat cacgctgcga ccactaacca tggcacaggt gcgcgcagcc
      481 ttgatcgatc aatggaaagt aaacaatgat caggcaaacc tgctggcgca cttatccggc
      541 ggccgcttag gctgggcgat caatgcggtt accgatcgat cgatcatgga aaaccgatcg
      601 aagttgctgg acgatctcgt tcaattattg aagagcgatc gcgtggcacg ttttgcgtat
      661 gccgaaggca tcacgcgcaa agacgggcgc acccgcgaag caatcgaagt ttggcagacg
      721 tggtggcgtg atgtgatgct ggcggccagt caaagtaccg ccgagccaat caacatcgat
      781 cgggccgcgg agcttgtcac catgtccaaa cagatcgatc tcgatcgcgc ccacgccgct
      841 gtcgaagcgt gcgcgaaggc aacatggcag ctcgaacgca atgcagccgc ccggcaggtt
      901 atggaaatct tgctgctgga ttttccgaaa gtgaagagtg agaagtgaca cttgcactgc
      961 atgctcgtgc agtgaagatg tgagtgaaaa gtaatgcgta gggcgtggtg cgtattgcgt
     1021 aaagacgaac cacgcaacag gcaccacgca tcacttattc ggtgacccgc cgattcgctg
     1081 attcactgta ttgtacgttg ccgttcaatt cgctccttca agcgctgcac tacttcatcc
     1141 ggcacaaaga tcgatcccaa ctccgcatgc gatttatcgc ccggcgaatg aaagtcgctg
     1201 ccgccggtaa cgatcagatc atactgctgt gccagcgaca acaactctgc gcgcaactct
     1261 ggagtgtgat cggaatagta aacttccacg ccatccaagc ccagctcaac aaactgcgta
     1321 actaaaggcg tggtctgggc atgcgccgga tgcgccagca cgatcgcccc acccgcgccg
     1381 tgcaccagat cgatcacttc ttgcggcgtt agttttaagc gatgcacata ggccggacca
     1441 tcatccgaaa tatatttatc aaaggcttct tgcatcgatg ccacatggcc tgcttcgacc
     1501 atcgcccgcg caatgtgcgg ccgcgcgatc gattgggcat cgcccgcgta tttcatcacg
     1561 cgatcccagt tcaccggcat acccatcgcg gctaattttt tgagcatgcc tttcgcgcgg
     1621 ccaactcgcg catcacgaat ggccaacaac tgatcttgca gcggcacatt ggatcgatcg
     1681 agaccgtacg ccagaaagtg cgtgtcggtc tgatcgtctt cgctgttgat ctccaagccg
     1741 acgatcaccg caagcccgat cgatctaccg gcatcgatcg cttcagccaa cccgtcggtc
     1801 gtgtcgtgat cggtcaaagc aatggtggtc aagcctcttg attttgccaa ctgaacaact
     1861 tccgacgggc gcaaggtccc atcggatgcc gtgctatgca tatgtaagtc aacatgattg
     1921 gtcatgccgc gcattctacc acacccgatc gaccgatctt ttttcgcgca gaccaagcac
     1981 caatgctacc gggccgatca tcaacagcca catcgccgat tgcagtccga tcgcatcggc
     2041 gatgccgccc aagatcagcg gtacaaacaa attaatcccg ccgatgatcg acgtcacggc
     2101 aatcaccgcg cccgatcgac caggcagcgc ttcatacgat ttcgcccgca agatcgtata
     2161 ctaa
//
LOCUS       DASRTJ010000192         3880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_313713,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000192 DASRTJ010000000
VERSION     DASRTJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313713"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1298
                     /locus_tag="VFF70_04060"
     CDS             126..1298
                     /locus_tag="VFF70_04060"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZY43903.1"
                     /translation="MNKKTIRDIDFTNKRALVRVDFNVPIKDGAVTDDTRITAALPTI
                     KYLIDHKAKVILMSHLGRPKGGPDPKYSLSPTAIRLSQLLDQNVQMAPDCVGTEVEKM
                     VNALKPGDVLVLENVRFHPEEEKNDGAFADQLAKLGDIYVNDAFGSAHRAHASTEAVA
                     HRLPAVAGFLMEAEIDYLGRALEKPDWPFVAILGGAKVSDKIAVIENLLTKVDQLLIG
                     GGMANTFFKAQGLEVGDSLIEETAISTAKDLIARGGEKLKLPIDVVIADKFDADAQMK
                     TVQIDQVPTSWRILDIGPQTVSMYNEIISKAKMIVWNGPMGVFEFPRFAAGTNAVARA
                     IAASQATSIVGGGDSAAAVQNSGVADKITHISTGGGASLEFLEGKVLPGIAALNDR"
     gene            1385..1816
                     /locus_tag="VFF70_04065"
     CDS             1385..1816
                     /locus_tag="VFF70_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43904.1"
                     /translation="MKDTHVDSDRMLFAAVLISGFVGLIIFLLVYTTILQYGTLNENG
                     KAYARVGLYVLAGTLILSLYLALAARSRSLTTPGMQGLGWLLVFGGFILACPALGGTI
                     GLKVIPGVTGFSGVAGAMFMIISGSSLLQAERNARDRNKEN"
     gene            1788..2261
                     /gene="rsfS"
                     /locus_tag="VFF70_04070"
     CDS             1788..2261
                     /gene="rsfS"
                     /locus_tag="VFF70_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZY43905.1"
                     /translation="MPVTETKKTKSKPRTATTTKKRATVRKVAKPIKQPTPAEIENTA
                     LLDLAHGIVEAISDKLGEKIVLIDLRQHTPFTDYFVICSANSTRQIKAIHDGVREHTI
                     KHFNAKPRHIEGSTDSGWVLMDYSGIVVHIFAPTQRQFYQLEDLWKESPVVLKMQ"
     gene            complement(2337..2786)
                     /gene="ndk"
                     /locus_tag="VFF70_04075"
     CDS             complement(2337..2786)
                     /gene="ndk"
                     /locus_tag="VFF70_04075"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZY43906.1"
                     /translation="MERTLVILKPDAVQRGLIGPITARLEQRGLKLVGMKFIRVMPDL
                     AAKHYAVHQGKPFYDGLIKYITSSAVVVQVWEGKRAIQVVRNACGATNPAEAAAGTIR
                     ADFGVEIGRNLIHASDAPETALYEIPLYFTDSELVEWDRAIEPWITE"
     gene            complement(2870..>3880)
                     /locus_tag="VFF70_04080"
     CDS             complement(2870..>3880)
                     /locus_tag="VFF70_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43907.1"
                     /translation="DAIMGRKTTPQSEMPLTPLPVVETTASAAETVPEEIPGADDALA
                     FLTKLSAGKEDQLQTPGDEMGQARQAVFAKPAEIQVDGAATQSSLPITPTTPQQAMSN
                     ADALEFLQSIVADKEAHEPQPSMPVSTPIDSEIEPAPAEPVSFTIPKAEEPIASISTA
                     SVAEASTSTALPTWWWIQTAEEEDEEPIAELPEPYLPPRTPASAVPATKRERTSTARE
                     KVRAGEPTASAKVIAPQNKPTINVDPLLERLQADSNDHDARLELARAWWSTGQRVQAL
                     EHYSVLVEADAHPDETVNDLERIVEIEDRSEWQRLLGDVYRNTGQLTRALDAYHRALN
                     QL"
BASE COUNT          905 a         1030 c         1039 g          906 t
ORIGIN      
        1 gtgtccgcac tgcggacctc gccaacctcg tcgcctcaaa actctaaaac ggaggatgga
       61 agctggaagg tgaggttgga cgcagcgctc ttccaacttt catcctttca ctttcaatat
      121 tcaaaatgaa taagaaaacg atacgcgaca tagacttcac caacaaacgt gcgctcgtgc
      181 gcgtcgattt taacgtgccg atcaaagatg gggccgtcac cgatgacacg cgtatcactg
      241 cggcactgcc caccatcaag tatctcatcg atcacaaggc caaagtaatt ttgatgtcgc
      301 acctcggtcg accgaaagga ggccccgatc cgaaatactc tttgagcccg actgccatcc
      361 gattgagcca gctgctcgat caaaatgttc agatggcacc cgattgcgtc ggcactgaag
      421 ttgaaaaaat ggtgaacgca ctgaagccgg gcgatgtact cgtgcttgaa aacgtccggt
      481 tccatcctga agaagaaaag aatgatggcg cgttcgccga tcaactggcg aagttaggtg
      541 acatctatgt gaacgatgcc tttggctcgg cacatcgcgc gcatgcttcg accgaagcgg
      601 tggcccatcg attgcccgcc gtggctggat ttttgatgga agccgagatc gattatcttg
      661 gtcgcgcgct cgaaaaaccc gattggccct tcgtggcaat tctgggtggc gcaaaggtct
      721 ctgacaagat cgcagtgatc gaaaacttgc taaccaaagt tgatcagctg ctgatcggcg
      781 gcgggatggc gaacacgttc ttcaaagcgc agggactgga agtcggcgat tcgctgatcg
      841 aggaaaccgc gataagcacc gctaaggatt tgatcgcgcg tggcggtgaa aaattgaagt
      901 tgccgatcga tgttgtgatc gccgacaagt tcgacgctga cgcccagatg aaaaccgtcc
      961 agatcgatca agtcccaacc agttggcgca ttctcgatat cggcccgcag accgtatcga
     1021 tgtataatga gatcatctcc aaggcaaaga tgatcgtctg gaacggacca atgggcgtgt
     1081 ttgagttccc gcgttttgcc gcaggcacga atgctgtcgc gcgtgccatt gccgcctcac
     1141 aagcaacgtc cattgtcggt ggcggcgatt cggcagcagc ggttcaaaat tcgggcgtgg
     1201 ccgataaaat cacgcacatc tcaaccggcg gtggggcgag tttggaattt ctggaaggca
     1261 aagtgctgcc cggtatcgca gctctgaatg atcgataatt ttcatccacc cgcagtttga
     1321 tcttggcacg atcgagtttg caaaaacccg cgggaggatc gtaaacccat agggagactg
     1381 cacgatgaaa gatacgcacg tcgattcgga tcgaatgttg tttgcggcag ttttgatcag
     1441 cggctttgtc gggctgatca ttttcttgct cgtctacacg acaatcctgc aatacggcac
     1501 gctcaacgag aatggcaagg cgtatgcacg cgtcggtctg tatgtgctgg ccggtacgtt
     1561 gattctgtca ttgtatctcg ccttagccgc gcgcagccga tcacttacga caccgggcat
     1621 gcaaggcttg ggctggctgc tggtttttgg cggcttcatt ctggcttgcc cggccctcgg
     1681 cggtacgatc ggcctaaaag taattccggg cgttactggt ttcagtggtg tggccggggc
     1741 catgttcatg attatttccg gcagttcact tttgcaagca gaacggaatg cccgtgaccg
     1801 aaacaaagaa aactaaaagc aagccgcgca ctgcgacgac aactaaaaaa cgggcaaccg
     1861 tccggaaagt ggctaaaccg atcaagcagc caactccggc ggaaattgaa aacactgcgc
     1921 tgctcgatct agcgcatggt attgtcgaag cgatttcaga caagttgggc gaaaagatcg
     1981 tcttgataga tctgcggcag catacgccgt tcaccgatta ctttgttatt tgctcagcca
     2041 acagcacccg ccagatcaaa gcgatccatg acggagtccg tgaacacacg atcaaacatt
     2101 tcaacgcaaa gcctcgccac atcgaaggct caaccgacag cggctgggta ttgatggact
     2161 acagcggcat cgtcgtgcat atctttgcac ccacccagcg ccagttttat caattagaag
     2221 atctgtggaa agaatctccg gtggtattga agatgcaata gtaagccaag atgccaactg
     2281 ggcgcacaac aaaaagacct ccaaaattgg aggccatttg ttttatcagc tccaggttat
     2341 tccgtgatcc acggctcgat cgcgcgatcc cactcaacaa gttcgctgtc ggtgaagtat
     2401 aacggaattt cgtacagagc tgtctcaggc gcgtcggacg catgtattaa attccgcccg
     2461 atctcaacac cgaagtcagc gcgaatggtg ccggctgcgg cctcggcagg attggtagcg
     2521 ccgcaagcat tgcgcacaac ctgtatcgcg cgtttgcctt cccaaacctg caccaccact
     2581 gcacttgagg tgatgtactt gatcagccca tcataaaatg gtttaccttg gtgcacggca
     2641 taatgcttgg ctgccaaatc gggcatgaca cgaataaatt tcatgccgac caacttgagg
     2701 ccgcgctgtt ccaatcgggc ggtgatcgga ccgatcaaac cgcgctggac tgcatcgggt
     2761 ttcaaaataa cgagcgtgcg ttccactgaa atgttctcct cgcagacttg agattgattt
     2821 gatattgaca gcaaccgacc gcgttgccgc ggtcggtaaa tcttgtggct tacagctgat
     2881 tcaacgcacg gtgataagca tctaaggcgc gtgtcaactg gccggtattc ctgtatacgt
     2941 caccaagtaa acgctgccac tccgatcgat cctcgatctc gacgatgcgc tctagatcgt
     3001 taacggtctc gtctgggtgt gcatcagcct cgactaagac tgaataatgt tccaacgcct
     3061 gaacgcgttg acctgtcgac caccaggcac gggccagctc taatcgtgca tcgtgatcgt
     3121 tcgaatcagc ttgcaaccgc tccagcagtg gatcgacatt gatggtaggt ttattttgcg
     3181 gcgcgatcac tttagccgat gcggttggct cacctgcgcg caccttttca cgcgcagttg
     3241 aggtccgctc gcgcttagtg gctggcacag ccgaggcagg cgttcgcggt ggaagataag
     3301 gctctggcag ctccgcgatc ggctcttcat cttcctcttc ggccgtctgt atccaccacc
     3361 aagttggcag ggcagtggac gtagatgcct ctgcaacaga ggcagttgat attgacgcga
     3421 tcggctcttc agcctttggt atagtaaatg aaacaggttc ggcaggtgca ggttcgatct
     3481 cgctgtcgat cggcgttgat accggcattg atggttgtgg ctcgtgtgcc tctttatcag
     3541 ccacaatgct ctgcaaaaat tcgagcgcgt ctgcattaga catcgcctgt tgcggcgttg
     3601 tgggtgttat cggtaagctt gactgtgtcg cagctccatc aacctggatc tcggcaggct
     3661 tagcaaatac cgcttgacgg gcctgtccca tttcatcgcc aggcgtctgc aattgatctt
     3721 ctttaccagc agataattta gtcaggaatg ctaaagcatc atccgcgccg ggtatttctt
     3781 ctggcactgt ttcggcagca gacgcagttg tttcaacaac tggcagtgga gttagtggca
     3841 tctctgactg cggcgttgtc ttgcgcccca tgatcgcatc
//
LOCUS       DASRTJ010000193         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_49245,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000193 DASRTJ010000000
VERSION     DASRTJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49245"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFF70_04085"
     CDS             <1..439
                     /locus_tag="VFF70_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY43908.1"
                     /translation="SALDHGRLGVAAGALGVAQASLDACVEFARTRKQFGQRIGNFEM
                     IQSTLADMAADVEAARLLVYRAAWLKDQNIKATRETSIAKLFATEAAVKAAGEAVLLH
                     GGRGYSNEYPVERYYRDIKGLQIYEGTAHIQRLVIARELLGRE"
     gene            468..2015
                     /locus_tag="VFF70_04090"
     CDS             468..2015
                     /locus_tag="VFF70_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HZY43909.1"
                     /translation="MKASDLPLTYNAVNILEHNLTSRADKVALYTADRELTFQQVANE
                     VNQVGHALKKLDVRMGESVGILSLDCAEWVTSFFGVVKIGAVAVGMNTLLKPPEHAYI
                     LRDARVRTLIVHSAVLPAIEPIRDELEFLTHVIVIGGTGRAGDFVFKDWIKGESTELG
                     VAPTHRDDFCSLNYSSGTTGEPKGIFHAHKDYPLTAQLWGVDVLGIRESDRAFGAPRL
                     FFTFGTGGVLIFPWYVGASTILMSGPPRIVTNVLDTINRFKPTLLYNAPTGYAALLAL
                     EDLSDKCDLSALRLCVSAGESLPASIWHAWKERTGIDLIDGIGSTENYHIFISNRPGD
                     IRPGSSGKPVAGYEVKIVDEDDREVKQGEVGNLLVKGETASLFYLHQYERSKRTFLGD
                     WLSTGDKYFVDEDGYYWHAGRSDDMLKVGGIWVSPVEVESALIGHPAVLECAVVGHAD
                     ASNLIKPKAFVVLKEGHAPSAELKAALIDHCKEKMAEYKRPRWIEFVPELPKTATGKI
                     QRFKLRG"
     gene            2231..>2830
                     /locus_tag="VFF70_04095"
     CDS             2231..>2830
                     /locus_tag="VFF70_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZY43910.1"
                     /translation="MKTIVYIDGFNLYYGAIKGTPYRWMNPLALCRLLLPQDQIQSIK
                     YFTARVNPRPHDPDQRIHQETYWRALRTLPSLSIILGFFLTHEISMPLAGNPNRQVQV
                     IKTEEKGSDVNIATHLLIDGFKNNYELAVIVSNDSDLLAPIRFVVEELHKPIGLLNPQ
                     KHPSVELSKYVTFIKQIRGGVLAKSQFPPMLKDAAGKFSK"
BASE COUNT          653 a          733 c          819 g          625 t
ORIGIN      
        1 gtccgcgctg gatcacggtc gattaggagt ggcggcgggc gcgttgggcg tcgcgcaagc
       61 gtcgctcgat gcgtgtgtag aatttgcgcg gacgcggaaa cagttcgggc agcgcatcgg
      121 caacttcgag atgatccaaa gcacgctggc ggatatggcc gcggatgtcg aggcggcgcg
      181 actgttggtg tatcgcgcag cgtggttgaa ggatcaaaac atcaaagcga cacgcgaaac
      241 gtccatcgcc aaactttttg caaccgaggc ggcggtgaaa gcggcgggcg aggcggtctt
      301 gctgcatggc gggcgcggct actcgaacga atatccggtg gagcgttact accgtgacat
      361 caaaggactt caaatttatg agggcacggc gcacattcaa cggttggtga ttgcgcgtga
      421 actgttaggg cgcgaataaa ttttattaaa cgaaaggaag ccgatcgatg aaagcctctg
      481 atcttccact cacttacaat gcagtcaaca ttctggaaca caacctgacc tcgcgcgccg
      541 ataaagttgc gctgtacacc gccgatcgcg agttgacctt tcagcaagtg gcgaatgaag
      601 tcaaccaggt gggccatgcg ctgaaaaaac tcgacgtgcg catgggcgag agcgtcggca
      661 ttttgagcct cgattgcgct gagtgggtca cttcgttttt tggcgtggtc aagatcggcg
      721 cggtggcggt gggcatgaat acgttgctca agccgccaga gcacgcgtat attctgcgcg
      781 atgcccgcgt gcgaactttg atcgttcaca gcgcggtgct gcccgcgatc gaacccatcc
      841 gcgacgagtt ggaatttctg acacacgtca tcgtcatcgg cggaacgggc cgcgcaggcg
      901 attttgtttt caaggactgg atcaagggcg aatcaaccga gctgggtgtc gcgccgacgc
      961 atcgcgatga tttttgttcg ctcaattatt ccagcggcac gaccggcgag ccgaaaggga
     1021 ttttccacgc gcacaaagat tatccgttga ccgcgcagtt gtggggcgtg gatgtgttgg
     1081 gcattcgcga aagcgatcgg gccttcggcg cgccgcgctt gttcttcacc tttggcacgg
     1141 gcggcgtgtt gatcttcccg tggtatgtcg gcgccagtac gatcttgatg tccgggccgc
     1201 cgcgcattgt cacgaacgtg ctggacacga tcaatcgctt caagcccacg ctgctgtaca
     1261 acgcgccgac cggctatgcc gcgctgttgg cgctggaaga tctatcggac aaatgcgatc
     1321 tctcggcctt gcgtctgtgc gtctcggccg gcgagtcgct gccggcatcg atctggcatg
     1381 cttggaaaga acgcacgggg atcgatctca ttgacggcat cggctcaact gaaaattatc
     1441 atatcttcat ctcgaaccgt ccgggcgata ttcgacccgg cagcagcggc aaacccgtgg
     1501 cagggtacga agtcaagatc gtggatgaag acgatcgaga agtgaagcaa ggggaagtcg
     1561 gcaatctgct cgtcaagggc gagacggcct cactctttta tttgcatcaa tatgagcgca
     1621 gcaagcgtac tttcctgggc gactggttga gcacgggcga taaatatttt gtggatgaag
     1681 acggttacta ctggcacgcg ggacgatccg atgacatgct gaaagtgggc ggcatctggg
     1741 tctcgcccgt cgaagtggag agcgcgctca tcggtcatcc ggcggtgctg gagtgcgcgg
     1801 tggtcggtca cgcggacgcg tcgaacttga tcaagcccaa agcgtttgtc gtcttgaaag
     1861 aagggcacgc gccttcggct gaattgaagg cagcgttgat cgatcactgc aaagaaaaaa
     1921 tggcggagta taaacgcccg cgctggatcg agtttgtgcc cgagctgccc aaaacggcca
     1981 cgggcaagat acaacgcttt aagttgcgcg gctaagctaa cacactgacg ccgcatcgat
     2041 aatcggtgcg gcgtctccgc gcgaatcgcg aatatagatc gagtgcggaa aaaataattc
     2101 tcagcacctg aaatttgaca aagaatcaaa agtgatagat aatcctcgca catcccccag
     2161 tagtagcttc ggcgaacctg gtacgaagcc gccgcaaggc ggcttccgtg ttgatagagc
     2221 ctgactgcaa atgaaaacaa tcgtctatat cgatggtttt aatttgtatt acggcgccat
     2281 caaaggtaca ccctaccgct ggatgaatcc gttggcctta tgccgcttgc tcctgcccca
     2341 ggatcagatt caatcaatta aatatttcac cgcccgcgtc aatccgcgtc cgcatgatcc
     2401 cgatcaacgt attcatcagg aaacttactg gcgggctttg cgtacgctgc caagtctatc
     2461 gattatcttg ggcttcttct tgacgcatga gatttctatg ccattagccg gcaatcccaa
     2521 tcggcaagtg caggttatta agactgaaga aaaaggctcg gacgtgaaca tcgccacaca
     2581 cttgttgatc gatggtttca aaaacaatta tgaattagcc gtcattgttt ctaacgattc
     2641 ggatctgctg gccccaattc gatttgtcgt tgaagagctg cacaagccga tcggcttgct
     2701 taatccccaa aagcatccca gcgtcgaact atcgaaatac gtgacgttca tcaagcaaat
     2761 tcgcggcggc gtcttagcca agagccagtt tccgcctatg cttaaagatg cggctggaaa
     2821 gttttcaaaa
//
LOCUS       DASRTJ010000194         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269793,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000194 DASRTJ010000000
VERSION     DASRTJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269793"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..772)
                     /locus_tag="VFF70_04100"
     CDS             complement(98..772)
                     /locus_tag="VFF70_04100"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY43911.1"
                     /translation="MAGWLLRSIIGTIVYGSIIFLPAGTFNWLWGWVMLGILVAVMIV
                     QPLMLMRINPAVLIEREKSFWTQGVKRWDKWITTIAGGLMPQPWIIAGLDVRFQWTAS
                     MQLTVHIGGLIFVVIGHALFLWAMAANAFFSQGARIQSERNQQLVDSGPYRFVRHPGY
                     AGVILYSIATPILLGSMWALIPGILSAILFGVRTRLEDKMLMTELPSYTEFAQRTSFR
                     LLPGVW"
     gene            complement(850..>1632)
                     /locus_tag="VFF70_04105"
     CDS             complement(850..>1632)
                     /locus_tag="VFF70_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZY43912.1"
                     /translation="DPNLQNIPIRTDLGREVRRGFIVEKGHVMIAADYSQVELRIAAH
                     IASDPGLRAAFAAGEDIHRATAAKVIGIPLDQVTGDQRSFAKRVNFGLLYGMGTQSLA
                     QQAGISMKEAQKFVDAYFSGFPNIRKYIDDTKRKANEIGYVETLLGRRRYFPILKTET
                     RDARTNIMQRSAEREAINHPIQGTAADIIKIAMINIHAALKKKKMETRLILQVHDELI
                     LEAPKDEADEAAKLVKKLMETAYDLDPALKVEVGLGQNWDEV"
BASE COUNT          370 a          454 c          411 g          397 t
ORIGIN      
        1 agcctgcatc ccatcggctc gatcgtgcgg ctggctcaat cggcgaaagt gtttccgaag
       61 attggggaat atcgaaggta agacatttgg cgaggtttta ccacacgccc ggcagtaatc
      121 gaaacgacgt gcgttgtgcg aactctgtgt aactcggtaa ttctgtcatc agcattttat
      181 cttccaaacg agttcgtacc ccgaacaaga tcgcagacaa gattccgggg atcaatgccc
      241 acatcgatcc gagcaagatc ggtgtcgcga tcgaatacag gatgacaccc gcgtaacccg
      301 gatggcgcac aaatcgatat ggtccgctgt ctaccagttg ttgattgcgc tccgattgga
      361 ttcgagcccc ttgcgagaaa aaagcattag ctgccatcgc ccataaaaaa agcgcatgtc
      421 ctatgacgac gaagatcaag ccgccgatgt ggactgttaa ctgcatcgat gctgtccact
      481 gaaagcgcac atccagtccg gcgatgatcc atggttgcgg catcaagccc ccggcgatgg
      541 tcgtgatcca tttatcccag cgcttcacgc cttgagtcca aaaacttttc tcacgttcga
      601 tcaataccgc cggattgatg cgcatcaaca tcaatggctg aacgatcatc accgcgacca
      661 ggataccgag catgacccaa ccccacagcc agttgaatgt accggctggc aggaagatga
      721 tcgatccgta gacgatggtg ccgatgatcg atcggaggag ccatccagcc atcaatgatc
      781 gtgtggttgt tttgaacctc aatccgccat cagagttcat ctcaatctcg atctcactta
      841 gcagtttctc tacacctcat cccaattctg ccccagcccg acttccactt tcaacgccgg
      901 atcgagatcg taggcagtct ccatcaactt ctttaccaat ttcgccgctt cgtcggcttc
      961 atccttcggc gcttcgagga tcaattcatc gtggacttgc aggatcaagc gcgtctccat
     1021 ctttttcttc tttaacgcag catggatatt gatcatcgcg atcttgatga tgtccgcggc
     1081 ggtgccttga atcggatgat tgatcgcttc acgttcggcc gatcgctgca tgatgttcgt
     1141 acgcgcatca cgcgtttcgg tttttaaaat cggaaaatat ctgcggcggc ccaataatgt
     1201 ttcgacgtag ccaatttcat tcgctttgcg tttggtgtca tcgatatact tgcggatatt
     1261 cgggaagccg ctgaaatatg catcgacgaa cttctgcgct tctttcatcg agatgccagc
     1321 ttgctgcgcc agcgattgag tgcccatgcc gtagagcaag ccgaagttca cgcgctttgc
     1381 aaacgaacgt tgatcgccgg tgacttgatc gagcggaatg cctatcactt tggccgccgt
     1441 cgcgcggtga atatcctcgc ctgcggcgaa cgcggcacgc agacctggat cgctggcgat
     1501 gtgcgcggcg atgcgaagtt ccacctgaga ataatcagcg gcgatcatca cgtggccttt
     1561 ctcgacgatg aagccgcgcc gcacttcgcg gccgagatcg gtgcgaatgg gaatgttttg
     1621 cagattcgga tc
//
LOCUS       DASRTJ010000195         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_269817,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000195 DASRTJ010000000
VERSION     DASRTJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269817"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFF70_04110"
     CDS             complement(<1..183)
                     /locus_tag="VFF70_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZY43913.1"
                     /translation="MKFRRSLISLLAVALILSSTASSTLASPLNACAAYHVVQAGENL
                     FRIGLQYGITFDVLMQA"
     gene            405..1811
                     /locus_tag="VFF70_04115"
     CDS             405..1811
                     /locus_tag="VFF70_04115"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HZY43914.1"
                     /translation="MTLVVSTPKYFDTVLETIGNTPLVRLNHVTAGVRCNVLAKLEFM
                     NPGGSVKDRIGLMMIEAAELDGRLKPGGTIVEATSGNTGMGLAIAAAIKGYKCIFVMP
                     DKMSHEKVQLLRAFGARVVVTPTAVEPHDPRSYYSVSQRIVAETPNSILANQYHNPMN
                     PESHYRTTGPEIWQQTGGQLKAFITGMGTGGTISGTGRYLKEQNPKVKIIGVDPIGSL
                     LYDTWRMGRVPAEPFLKTYKTEGIGEDFLPGATDMSVIDEIDQVSDKESMLMTRRLVR
                     EEGIFCGGSSGSAVAGLLKSPIVHALNSDEFVVVILADSGSRYLSKIFDDEWMRENGF
                     LESTWGEARVSDVLATKPSRLLVTASPSDRVRAVIDLMKQHDISQMPVLTDGKLVGMI
                     GESSLLTQMSTPGSSAEESIGPIINRQVTSVPTDTGLEAVMTLFGANQAVIVVDADRV
                     VGILTKIDLIDYLAAHMK"
     gene            1864..2706
                     /locus_tag="VFF70_04120"
     CDS             1864..2706
                     /locus_tag="VFF70_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZY43915.1"
                     /translation="MTPHQRETELPDLRIVPIANLLLHEQYDAQRSAPLAARLTSEGT
                     LKNPPIVAPIDDYRFVVLDGANRVTALAALGLQHIAVQLVDYEDPDLILDTWYHLVSG
                     FPRDEFSQAIGLLPGVKIEAVELLHARAELARRDALAYFALPGDSVFTIRAEGDVHQR
                     TQLLNQIVDVYQARGKIYRANTDHLDQLLPFYDDVTALVVFPQYQPAEIIELARVGAR
                     LPAGITRHLIPRRALRINVPLSMLREDRSLAEKNAWLKNWLKSKAVNKEVRYYQESTY
                     LFDE"
     gene            2792..>3028
                     /locus_tag="VFF70_04125"
     CDS             2792..>3028
                     /locus_tag="VFF70_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HZY43916.1"
                     /translation="MTTQQLGDMNSEEFRKYGKQLIDWIADYLDYPDRYPVLSQVLPG
                     DIKSKLPQQPPPSSEAMDKILNDFDTIILPGVTHW"
BASE COUNT          729 a          764 c          830 g          705 t
ORIGIN      
        1 ggcctgcatc aatacatcaa aggtaatgcc gtattgcaag ccaatgcgga agagattctc
       61 gcctgcctgt accacgtgat aagcagcaca agcgttaagc ggggaagcca gcgttgagct
      121 cgcagttgag cttagaatca acgcgacagc cagcagactg ataagggagc gacgaaattt
      181 catgggaatc tccttgagca gtgtcgacaa aaatatcccg accgttctag tgctaagcat
      241 acactataaa agcgtgctat gcaaactatt ttgacagtga ttttctttca tggtaagctg
      301 cattttgaag cacgaagtcg gcgccgagat cgatcgggtt ttgcgatata tctggcctct
      361 gccgcgctta atcgattcag attgtcctcg ataaaggatt gtgaatgacc ttagttgttt
      421 caaccccaaa gtatttcgac accgtgcttg aaacgatcgg caataccccg ttagtccgct
      481 tgaatcatgt gactgctggt gtgcggtgta atgtgttggc gaaattggaa tttatgaatc
      541 ccggcggatc cgtgaaagat cgaatcggct tgatgatgat tgaagccgcg gagttggatg
      601 gccgcttgaa gccgggcggc acgatcgtcg aagcaacctc cggtaatacc ggtatgggtc
      661 tggcgatcgc ggcggcgatc aagggttata agtgcatttt cgtcatgccc gataaaatga
      721 gtcacgagaa agttcaactg ctgcgtgcat tcggtgcgcg cgtcgtcgtg acgccgacag
      781 ccgttgagcc gcatgatccc cgatcgtatt acagtgtcag ccaacgcatc gtggccgaaa
      841 cgccgaacag catcctcgcc aatcaatatc acaatcccat gaatcccgaa tcgcattatc
      901 gaacgaccgg gccggagata tggcaacaga ccggcggaca gctcaaagcg tttattacgg
      961 gcatgggcac gggcggcacg atctcaggca cagggcgtta cttgaaagaa caaaatccta
     1021 aagtcaaaat tatcggcgtc gatccgattg ggtcgttact gtatgatacg tggcgcatgg
     1081 ggcgtgtgcc cgccgagccg ttcttgaaaa cctacaagac cgaaggcatc ggcgaagatt
     1141 ttctgccggg cgccacggat atgagtgtga tcgacgagat cgatcaggtc agtgataaag
     1201 aatcgatgtt gatgacgcgc cgcttggtca gagaagaagg tatcttctgc ggaggatcat
     1261 caggctcggc cgtggcgggc ttgctaaaat ccccgatcgt tcatgcgctc aactccgacg
     1321 agtttgtcgt cgtgatattg gccgactccg gttcgcgtta tctcagcaag atattcgatg
     1381 atgaatggat gcgggagaac ggtttcctcg aatcgacatg gggtgaagcg cgcgtgtcgg
     1441 atgtcttagc gaccaagccc agccgattgt tggtgactgc gtcaccgtcc gatcgagtgc
     1501 gcgctgtgat cgatctcatg aagcagcacg atatctcgca gatgccggtg ctgaccgacg
     1561 gaaaacttgt cgggatgatc ggcgaatcga gtctgttgac gcaaatgtcg acgcctggca
     1621 gcagcgcgga agaatcgatc gggccgatca tcaatcgaca agtgacgagt gtgccgacgg
     1681 ataccgggct ggaagcagtg atgacgcttt ttggcgcgaa tcaagctgtg atcgtcgtcg
     1741 acgccgatcg agtggtgggc atcctcacca agatcgattt gatcgattat ttggccgcgc
     1801 atatgaaatg atgcgtgatc acattcttag gcatcatgca tcacggagga tttattattt
     1861 atcatgactc ctcatcaacg tgaaaccgaa ttacccgatt tgcgtattgt gccgatcgca
     1921 aatctgctgc tgcacgaaca gtatgacgcg cagcgcagcg cgccactcgc tgcgcgactg
     1981 acctccgaag gcacgctcaa gaatccgccg attgttgcgc cgatcgatga ttaccgcttt
     2041 gtcgtgctcg atggcgcgaa tcgcgtgacc gctttggccg ccttgggctt gcagcacatc
     2101 gcggtgcagt tggttgatta cgaagacccc gatctgatct tggacacttg gtatcatctc
     2161 gtcagcggtt ttccgcgcga tgaattcagt caagcgatcg gtttgttgcc gggcgtgaaa
     2221 atcgaagcgg tcgagctgct gcatgcgcga gcagaattag cgcggcgcga tgccttggct
     2281 tactttgcgc tgccgggaga tagcgtattt accattcgcg ccgaaggaga tgtgcatcaa
     2341 cggacgcaat tgttgaatca gatcgtcgat gtttatcaag cgcgcggcaa aatctatcgg
     2401 gccaacacgg atcacctcga ccaattgctg cccttctatg atgatgtgac tgcgctggtt
     2461 gtgtttccac aatatcaacc ggctgagatt atcgaattgg ctcgcgtcgg cgctcgattg
     2521 ccggccggca tcacccggca tcttattcct cgacgggcgc tgcgcatcaa tgtgccgcta
     2581 tccatgctgc gtgaagatcg atcgctggcc gagaagaatg catggctgaa gaattggttg
     2641 aagagtaagg ccgtaaataa agaagtgcga tactatcagg aatcgactta cttatttgac
     2701 gaatgagagt atgactcgat gacacgatga ctgcacgcga gatcatcctg tcagcatgtc
     2761 accttttcac ttgtcacaat aacgaggcat cgtgaccact caacaactcg gcgacatgaa
     2821 ctccgaagag ttccggaaat atggcaaaca actgatcgat tggattgccg attacctcga
     2881 ttaccccgat cgctatccgg tcttgtcgca agtcttaccc ggcgacatca aatccaaatt
     2941 gcctcaacaa ccaccgcctt catccgaagc gatggataaa attctcaacg acttcgacac
     3001 catcatcctg ccgggtgtta cccactgg
//
LOCUS       DASRTJ010000196         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_11375,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000196 DASRTJ010000000
VERSION     DASRTJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11375"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFF70_04130"
     CDS             <1..659
                     /locus_tag="VFF70_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY43917.1"
                     /translation="TPLTTILSSTELLEHYSARMSEDRKLEHLRQISAAVKHMTDLME
                     DILIIGKAEAHMLAFVPGPIDLEKYCRDLVEEFQLTAGSQYHLTLISREQCLAANMDV
                     NLLRHILSNVLSNAVKYSPRGGDIEFELTCEEAWVVFRIRDHGIGIPQPDQSRIFESF
                     QRGSNTGNIAGTGLGLAIVYRSVALHHGTINIESEVGVGTTVTLKLPLNNSSTLQESS
                     "
     gene            656..1762
                     /locus_tag="VFF70_04135"
     CDS             656..1762
                     /locus_tag="VFF70_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43918.1"
                     /translation="MSKVLVIEDEADIRRNILDLLEVEGFAASSAENGLHGLRVIRAG
                     QIPDLILCDIMMPEMDGYGVLEELRKDLATSAIPFIFLTAKADRSDLRHGMELGADDF
                     ITKPFTRDELLKAISSRLMRAHAVRESTEAEFDQLKRRIGTMLAHELRTPLTTVMGYT
                     DLALEDISTLSPEQLLNFLGQIKQGSARLARLVEDLLLLTQVDTGQIDQDFQANLNIC
                     RNLSDVIARTLELYQSSARALGLRFETEIDPQLPAVNLNQPLFATAFGHLLTNSIKFS
                     KGQADLVKVVARSAGDGVDLAVIDHGIGIQADEIPRLFKRFHQINRQKIEQQGAGLGL
                     AVARELIRMHGGDIYVESAPGSGSTFTIHLPALV"
     gene            2077..2544
                     /locus_tag="VFF70_04140"
     CDS             2077..2544
                     /locus_tag="VFF70_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43919.1"
                     /translation="MAHSILVVDDDQATITLIGLMLKRQGFVVYEAQSGPEALIFLSH
                     TIPDLVLLDVMMPAMDGYEVCRRIKANPQLKHLPVVMLSARFESASQMEGFRAGAIDY
                     IAKPISPSDLIAQLQAVFERSEREPKPNSAVVIAARRCAMPVKQDNGPIVGAR"
     gene            complement(2810..>3286)
                     /locus_tag="VFF70_04145"
     CDS             complement(2810..>3286)
                     /locus_tag="VFF70_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43920.1"
                     /translation="TYCYFAVAVAIDGQRSTALSPTCTTPKADPVPPHGGININGGAT
                     STPGPNVTLNLIAADTIDPHNDSEYGASYMTPPADSASGVADMMISNRSDMQGGAWEP
                     YAIAKAWTLDQASGLASVFVKYRDVASNESQVYAATIHVGPGSPGLHDVYLPVITR"
BASE COUNT          746 a          863 c          890 g          787 t
ORIGIN      
        1 gcacgcccct gaccactatt ctctcctcga ccgagttact ggaacattac agcgcacgca
       61 tgagtgaaga tcgcaagctc gagcatctgc gccagatcag cgcggcggtc aagcacatga
      121 ccgatttgat ggaagacatc ctgattatcg gcaaggccga agcccacatg ctagcttttg
      181 tgccggggcc gatcgatctg gagaaatact gccgcgatct ggtggaagag tttcaactca
      241 cggcgggcag ccaatatcat ctgaccctca tcagtcgtga gcagtgcctg gcagcaaaca
      301 tggatgtgaa tttgctgcgt catattctca gtaacgttct gtctaatgcc gttaaatatt
      361 caccgcgcgg cggggacatt gagtttgaat tgacttgtga agaggcttgg gtggtattcc
      421 gaattcgcga tcacggcatt ggtatacccc aacccgatca gtccaggatt tttgaatcgt
      481 tccagcgagg gagtaatact ggcaacattg ccggcaccgg cttgggctta gccattgtct
      541 accgatcggt cgcgctgcat catggaacga tcaacattga aagtgaagtt ggcgtcggca
      601 ccaccgtcac cctcaaacta ccgctgaata actcttcaac ccttcaggag tcttcatgag
      661 taaagttctg gtcatcgaag atgaagccga tatccgtaga aacatcctgg atttgctgga
      721 ggtcgaaggt tttgccgcgt ccagtgcgga gaacgggttg catggcttgc gcgtgatccg
      781 cgcgggccag attcccgatc tgatactttg cgacatcatg atgccggaga tggacggtta
      841 tggtgtcttg gaggaactgc gcaaggatct ggccacttcg gcgattcctt ttattttctt
      901 gacggccaaa gctgatcgat ctgatctgcg gcatggcatg gaattaggcg ctgatgattt
      961 catcaccaag ccttttacgc gcgatgaatt gttgaaagct atttcatcgc gactgatgcg
     1021 cgcgcatgcg gttcgtgaaa gcactgaagc tgagttcgat caattgaagc ggcggattgg
     1081 aacgatgctg gcccatgaac tgcgcacacc actgacaacg gttatgggct atacggattt
     1141 agctttggaa gatatttcca cgctttcacc ggagcagtta ttgaacttct tagggcaaat
     1201 taaacaaggt tcggcccgcc tggcacgttt agttgaggat ttgttgttgt taactcaagt
     1261 ggataccgga caaattgacc aggattttca agccaacctt aatatttgtc gaaacttatc
     1321 ggatgtcatt gccagaacac tcgagttata tcaatcttcg gcgcgcgcac tggggcttcg
     1381 attcgaaact gaaattgatc ctcagttgcc cgccgtcaat ttaaaccagc ctttattcgc
     1441 cacggctttt gggcatttgt tgaccaatag catcaaattt tccaaaggtc aggctgattt
     1501 ggtgaaagtt gtcgcccgct ctgcagggga tggagtcgat cttgcggtga tcgatcacgg
     1561 cattggcatt caagccgacg agattccgcg tctattcaaa cgcttccacc aaatcaatcg
     1621 gcaaaaaatc gaacagcaag gcgcaggact tggcctagcc gttgcgcgtg aattgatcag
     1681 gatgcatggt ggtgatattt atgtcgaaag tgctcccggc tccggcagca cgttcacgat
     1741 ccatttgccg gcgttagtgt agttgcgggc aactacctga aaaagtcatt gagcgtggca
     1801 ataatcgatc ggggtggagc agggcgacca gtactttttc accgctcagg cgcttgcggt
     1861 acagtgccag gacattcgcc tgtcatttcg aacgacactt gcaccctcgt acaacggtgc
     1921 aatgcaagtg agtgagaaat ctcggtctcg atgaccaagt cttgctcatg atcgtcggga
     1981 tttctcgcaa aaagggctcg aaataacagc tgagcgtaag tccgaacatc gttcaggatg
     2041 ctgcaaaaaa gtttacgaat ttgtcgggag ggtatgatgg cgcacagtat attggtagtc
     2101 gatgacgatc aagctaccat caccttgatc ggcttgatgt tgaaacgcca aggctttgtg
     2161 gtgtacgaag ctcaaagcgg accagaagcc ctcatctttt tatcccacac cataccggat
     2221 ctggttttgt tggatgtgat gatgccggct atggatggtt acgaagtttg ccggcgcatc
     2281 aaagcgaatc cccagttgaa gcatttgccg gtggtcatgc tcagcgctcg atttgaatcc
     2341 gccagccaaa tggaaggctt tcgcgccggg gccattgatt acattgccaa acccatttca
     2401 ccgtctgatc tgatcgcgca gctgcaagcg gtgttcgaac gatctgagcg cgagccaaaa
     2461 ccgaacagcg ctgtcgtgat cgcggcgcgc cgctgcgcta tgccggttaa acaagacaat
     2521 gggccgatcg taggggcgag gtgaccactt ccttacatat caaacggtct gccagttttt
     2581 ctgtgttgga caagtagcca accgatacac tgccgacagc gaaatagact cgtactgtaa
     2641 ctactcagcc cgcatcctgc ccatgcccga cgcttcgcgt cgatgggccg ccagctcatc
     2701 gagcgctcgt ccgctgtggc accagcgccg ttgcgatgaa tgggcgctgg tacacttact
     2761 caaactgcct atcgcacggc gttgacgcct ggctgtgcca gggcacccat caacgcgtga
     2821 ttacaggcag gtagacatcg tgcaagccgg ggcttcccgg tcctacgtga attgtcgcgg
     2881 cgtacacctg cgactcgttg ctggctacgt cacgatactt cacaaacacg ctcgccagac
     2941 ccgacgcttg atccagggtc cacgctttcg ctatcgcgta cggctcccac gcgccgccct
     3001 gcatgtcact ccggttgctg atcatcatat cggccacgcc actcgcgcta tccgcaggcg
     3061 gcgtcatgta actggcgcca tattcgctat cgttatgcgg atcgatcgta tcggccgcaa
     3121 tcagattcag cgtgacattg ggtcccggcg tggaggttgc tccgccgttg atattgatac
     3181 cgccatgcgg cggcacggga tcagccttgg gcgtcgtgca ggtgggcgag agcgccgtcg
     3241 aacgttgccc atcgatcgcc acggccacag cgaagtagca ataggt
//
LOCUS       DASRTJ010000197         8967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_86864,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000197 DASRTJ010000000
VERSION     DASRTJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86864"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..653
                     /locus_tag="VFF70_04150"
     CDS             189..653
                     /locus_tag="VFF70_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZY43921.1"
                     /translation="MTHLKTATAFSTVDLASTVHGHGHRLTPQRHVILEAVRKAGGHL
                     TPDTVYTRVHAKNPAINRATIYRTLDFLCEMRLVVAMQWGGQKYYEAAGAHPHHHLVC
                     RNCNRIVQIDHGLIETLTEEVQKKHHFKVDMDHVALFGLCAECRRPGKQDKI"
     gene            681..1724
                     /gene="cbiM"
                     /locus_tag="VFF70_04155"
     CDS             681..1724
                     /gene="cbiM"
                     /locus_tag="VFF70_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HZY43922.1"
                     /translation="MLHSPQYMHIPDGYLSPMTCLVLLLLTLPFWYRGAKKLRQTMNA
                     RSVPLIALLAAFSFVIMMFNVPLPGGTTGHAVGGVLAAIILGPEIAAIAISIALLIQA
                     LFFGDGGILAFGANCFNMAVVLPYVSFAIYRAIGGKSGLADGRRIVAAAVGGWVGLTV
                     ASFFTAVEFGIQPFLFHAADGTPLYAPYPLSVSIPAMVVPHVLVASVIEGVITALVVA
                     YLQRSNQPILEVAEKPQLVAEAGSLRRLRALYVVLAVAVVAVPLGLLAPGTAWGEWGS
                     DELAGLGLQAVPQGLAQLENLWSAPLSGYDLPALGDSNAGYILSAIVGILIVAIVAWL
                     FTRLLTRNPAAKA"
     gene            1726..2583
                     /gene="cbiQ"
                     /locus_tag="VFF70_04160"
     CDS             1726..2583
                     /gene="cbiQ"
                     /locus_tag="VFF70_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZY43923.1"
                     /translation="MMMLDSGSKAAKAHKRHGVIEHTLSEISHTLEQSIFAEEIARQR
                     GLLQSLDPRVKVVSLIALLIAVGLSHNLAAIIGLYFFSLILARASSVPMGFFIKRVWL
                     FMPFFTGIIAIPALFITPGPALVQLPLGLVITRTGALTALFLLLRVSTSVSLAILLVL
                     TTPWNTVLKALGVLRVSDVFVLILGMTYRYIYLLLHTMNDMFLSRKSRVTGRLSSGDE
                     RHLIAASAGTLLGKSLHLSSEVYLAMQSRGFRNTPRTMDTFKMRTMDWVYLFSALAIT
                     ALAIWLGSL"
     gene            2589..3425
                     /locus_tag="VFF70_04165"
     CDS             2589..3425
                     /locus_tag="VFF70_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43924.1"
                     /translation="MDDQAIFDFKDVTFAYDGKVKAVEGVSLCIHAGERVAILGSNGS
                     GKSTLLKLMDGLYFPDTGTACAFGTPLTEQAFQDDAFNFSFRQRVGLVFQDSDVQLFS
                     PSVWDEVAFGPLQLGLSRDEVTQRVDAALTSLRIEKLRDRAPHRLSGGEKKRVALASV
                     LSFNPEIWLMDEPSAGLDPRSQSWLVDFIIEQGQAGKTIVTATHDLNIVDSIADRVYV
                     VNEEHHIVANGTPEEILANHDLLIASNLMHAHRHRHQRGEAEHTHPHIHLPLHQHEHD
                     HI"
     gene            3584..6592
                     /locus_tag="VFF70_04170"
     CDS             3584..6592
                     /locus_tag="VFF70_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZY43925.1"
                     /translation="MTDDSHDTKPTKPQGGWGSDQPTTPASLSKRSALDDVSLPASSS
                     WGQSKKSAPPPPPPQKIDRSRSNNRAPRSRLTAYGASRFLFIAGLFGVIMLVCACAIG
                     VVGYVTIAGTLPSPTELAKRATELFTSSQIYDRNGNLLYELVDPQGGRRTTVPLNKIS
                     PWLTKATIDTEDPRFYSHPGFDPIGIIRAVFQNARSGTEASGASTIAQQLVRNLLLPE
                     GAERTLNRKIREAILAAEITRQYPRDTILELYLNSINYGNLAYGIQAAAETYFHKDAA
                     ALTLAEASFLAGIPQAPAVYDPFTTDGLKYTLNRQKDVLRLMQQAGDLTANETKIASD
                     AMQNFVFTPPSNTFATSAPHWVVYVKQLVEEEFGPEALYRGGLKIYTTLDPKLQSLAE
                     QVVDDQIAQLGDKHVTDAGLVAMDPRTGEILAMVGSPDFNNVAISGQVNMTLRTRQTG
                     SVIKPITYLTAFEKGWTPSTVLWDVPVVYTDTAGNIYTPGNYDGRFHGPQWVRSTLAN
                     SYNIPAVKTLVNVGIPQFLDVARRLGIDTLTRPDYGPALTLGGGEVPLIEMTGAIAVL
                     ANQGVYLPPIALDRVEKADGSIVCKFTPPGQDNQNVPLCQPIENTGAQLVKPQQAYLL
                     TNILSDNAARTPAFGPNSALVLSRPAAVKTGTTNDFKDNWTIGYTPDLIAGIWVGNAD
                     NTPMEHISGVTGAGPIWHNFMEQALADRPVLDFARPSGIVEMEVCADSGIQPSQYCPS
                     RKREIYFADQLPPGPDHDWYQLLNCDGNQQVMLVVPDEAREWVNQNPDWLASKGVPLA
                     PIGNCAPGQPNGNATVNITSPADGSTVNGVISIIGSVTLDNFDHYQFEQGVSFDPGGW
                     EWVSGPYQAQVTNGELAQWDTRSVNDGEHTLRLTAFGKGGGKTEYRLHVIVQNNGPTP
                     TATDTPPPTTTPGPTLTDTPLPLPTLPPSTATPTPTATSPIPTDTPTNIPLPTDTPTL
                     APSPTDTPTPTATTETPPTETPTEVPTVSP"
     gene            complement(6815..7714)
                     /locus_tag="VFF70_04175"
     CDS             complement(6815..7714)
                     /locus_tag="VFF70_04175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY43926.1"
                     /translation="MQRFMRPVRLRADEIEVRPAQSSDQVAIGDLTENNRHTHFNLDW
                     WTFEDWLYPDRSSDAIWIAQHDTQPIGVAAIPIDDSPVAWIRSAAVADSYEPQSIFDA
                     LIDRATSHLRAQGVSSIVSIAFPDWYAAILTAAKFTPVVNVISLRKDDRSIPGSAAPS
                     TPDVATRPARSNDIRAIARNDRAAFDSVWWYSEKSIAHLLPIVAHFIVAEVDEQVVGH
                     AFSDMYGGQGHLIRLAVHPDFQQRGIGERLLIESLRYQVAAGAYPLTVNTQGNNLPSQ
                     KLYQRFGYRSIGVPVRIMQRSIS"
     gene            7859..8923
                     /gene="recA"
                     /locus_tag="VFF70_04180"
     CDS             7859..8923
                     /gene="recA"
                     /locus_tag="VFF70_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZY43927.1"
                     /translation="MPADGKNKALETTLASITKKYGEGAIMRLGEATHLEIESIPTGT
                     LALDLALGVGGIPKGRITEIYGPESSGKTTLCQHIVAESQRRGGTCAYIDMEHALDPN
                     YAAICGVDIENLYISQPDTGEQALEIADALIHSGAIDVIVIDSVAALVPRAEIEGEMG
                     DSHMGLQARLMSQALRKLSGAIKASNAAVIFTNQLRMKIGIMFGNPETTTGGNALKFY
                     ASIRLDVRRIQTIKGDGEATGNRVRVRVTKNKVAPPFREAEFDIMFNEGISKAGDILD
                     IGTELEIITKRGAFFAYGDTKLGQGRENAKEFLKAHVEVMTQIDQEIRAHAAKAAGKT
                     RLGGSGTEAEVASDKSEDEE"
BASE COUNT         1979 a         2426 c         2605 g         1957 t
ORIGIN      
        1 gacttgctgg ccaattccct catcgacatt ttgcgcagtg ctgcttagtg aaaaggcagt
       61 tacagatcat agcccggctt ccaggtgtga agttgggctt tttatttgtt tttgaaccaa
      121 atgggtcgat tgtgcgtata atgtggtaga atgcttattg cgactgttcg caataaggat
      181 gaatagcaat gactcacctg aaaaccgcta ctgccttctc aactgtagat ttagccagca
      241 cggtgcacgg ccacgggcat cgattgacgc cgcagcggca tgtgatcctg gaagctgtgc
      301 gaaaagcggg cgggcatttg acgccggata cggtctatac gcgggtgcat gccaaaaatc
      361 cggcgattaa ccgcgcgacg atttatcgga ctctggattt tttgtgcgag atgcggctgg
      421 tggtggccat gcagtggggc gggcaaaaat attacgaagc cgcgggtgca cacccgcatc
      481 atcatctggt ctgccgcaac tgcaaccgga ttgtgcagat cgatcacgga ttgatcgaga
      541 ccctgaccga agaggtgcaa aagaagcatc acttcaaagt tgacatggat catgtggcgt
      601 tgttcggctt gtgcgccgag tgtcgtcgtc ccggtaagca ggacaaaatc taaaagtcaa
      661 accaagaaag gaaactgatt atgctgcact caccgcagta tatgcacatc cccgatggat
      721 atctgagtcc gatgacctgt ctcgttctgt tattactgac gctgcccttc tggtatcgcg
      781 gtgcgaaaaa actgcggcag acgatgaatg cgcgcagcgt gccgctcatt gcgctgctag
      841 ccgccttctc gttcgtcatc atgatgttca acgtgccgct gccgggcggg acgaccgggc
      901 atgccgtcgg cggtgtgcta gccgcgatca ttttggggcc ggaaatcgct gctatcgcga
      961 tctcgatcgc attgttgatt caagcgttat tcttcggcga tggcggtatc ctggcctttg
     1021 gggcaaactg tttcaacatg gctgtcgtcc taccctatgt gagctttgcg atctatcgcg
     1081 cgattggtgg aaagagcgga ctcgccgacg gacgacgaat tgtagcggca gcagttgggg
     1141 gttgggtcgg actgacggtt gcatcgttct tcaccgcagt tgaatttggc atccagcctt
     1201 tcttgttcca tgctgctgat ggcacgccgt tgtatgcgcc ttatccgttg agcgtgtcga
     1261 ttccggcgat ggtcgtgccg catgtgttag tcgcgtcggt catcgaaggt gttatcaccg
     1321 cactggtcgt cgcctacttg cagcgaagta atcaacccat acttgaagtt gccgagaaac
     1381 ctcagctcgt agccgaggca ggcagcctca ggcgactgcg agcattgtat gttgtgctgg
     1441 cggtagcggt cgtcgcggtg ccgctgggct tgctcgcgcc gggcacggct tggggcgaat
     1501 ggggctcaga cgagctggcc ggcctcgggc tgcaggcggt tccgcagggg ctggcccaat
     1561 tagaaaattt gtggagcgcg cccttgtcgg gttatgatct gcctgcgtta ggggactcga
     1621 atgcgggtta cattctatcg gcgattgtcg gcattttgat tgtcgccatc gtcgcttggt
     1681 tgtttacacg attgttgacg cggaatcccg cggcgaaagc gtagcatgat gatgctcgat
     1741 tccggatcga aagctgcaaa agcccataag cgccacggcg tgatcgagca cacgctgtcc
     1801 gagatcagcc atacgctgga gcaatcgatc tttgccgaag agatcgcgcg gcagcgcggg
     1861 ctgctccaat ctctcgatcc gcgtgtaaag gtagtgtcgc tgatcgcgtt gttgatcgcg
     1921 gtcgggttgt cgcacaacct cgccgctatc atcgggctgt atttcttcag tctgatcttg
     1981 gcgcgggctt cgtcggtgcc gatgggtttc tttatcaaac gcgtgtggtt gttcatgccg
     2041 ttcttcaccg gcatcattgc catccccgct ttgttcatca cacctggccc ggcactggtt
     2101 caactgccac tcggactggt gattactcgc acgggcgcac tcaccgcgtt gttcctgttg
     2161 ttacgagtca gcacttcagt ctcgctggcg atcttgctgg tgctgacgac accgtggaat
     2221 accgtactca aagcgctcgg cgtgctgcgc gtgtccgatg tgtttgtgct gattctgggc
     2281 atgacctatc gctacattta cctgttgttg cataccatga acgatatgtt cttgtcgcgg
     2341 aagagccgtg tcacaggccg gttgagcagc ggcgatgaac ggcacttgat cgcggcaagt
     2401 gcgggcacat tgctgggcaa gtctttgcac ttgagcagtg aggtttacct ggcgatgcag
     2461 tcgcgcggct ttcgcaacac tccgcgcacg atggatactt tcaaaatgcg aacgatggac
     2521 tgggtctatt tgtttagcgc gctggcgatt acggcgttgg cgatttggtt ggggagttta
     2581 taggcagaat ggatgatcaa gcgatctttg attttaaaga tgtcaccttt gcgtatgacg
     2641 gtaaagtgaa agcggtcgag ggtgtcagtc tatgcatcca cgcgggtgag cgcgtggcga
     2701 tccttggctc gaacggatca ggcaaaagca cgctgctcaa attgatggat ggcttgtatt
     2761 ttccagacac gggcacagcc tgcgcctttg gcacaccgct gaccgagcaa gcttttcaag
     2821 atgacgcctt caatttttct tttcgacagc gcgtcggttt ggtctttcaa gacagcgacg
     2881 tgcagctgtt ttcgccatcg gtttgggatg aagtcgcctt cgggccgttg cagctgggcc
     2941 tcagtcgtga cgaggtgaca cagcgggttg atgctgcgct gacgtcgctg cgaattgaga
     3001 agctgcgcga tcgcgcgccg catcgtttgt ccggcggcga aaagaaacgc gtggctttag
     3061 cttcagtgct gagcttcaat cccgaaatct ggctaatgga tgaaccctcc gccggactcg
     3121 atccacgcag tcagtcgtgg ctggtagact tcattataga gcagggtcag gcgggcaaga
     3181 cgatcgtcac cgccacgcac gatctcaaca tcgtcgattc gattgccgat cgggtgtatg
     3241 tcgtcaacga agaacatcac atcgtcgcga atggcacgcc ggaagaaatc ctggccaacc
     3301 atgacttgct cattgccagc aacttgatgc atgcacatcg tcatcggcat cagcgtggcg
     3361 aagccgagca tacgcatccg cacatccatc tgccgctgca tcaacacgaa cacgatcata
     3421 tttaagcggc gactttcatc cgcttgacat acgcttcctc tacatcctcg ctacgctcgg
     3481 ttgacagccg taacttggcc gctataatcg cgttatatca gcatggcgcc cggaaccgca
     3541 ttcggcgggc cgggcgtttt atctgcattg atttggatcg acgatgactg acgactcgca
     3601 cgatactaaa cctaccaagc ctcaaggcgg ctggggatcc gatcagccca ccacgccggc
     3661 tagtctatcc aagcgcagcg cgcttgatga tgtttcgctc cccgcatcga gcagctgggg
     3721 acaatcgaag aaatctgcgc caccgccacc acccccgcag aagatcgatc gatcgcgatc
     3781 gaataatcgc gcgccacgat cgcgtctcac ggcttacggc gcttcgcgct ttttgttcat
     3841 cgccggatta tttggcgtga tcatgcttgt gtgtgcgtgc gcgatcggcg tcgtcggcta
     3901 cgtgacgatc gcgggcacgc tgccctcgcc gaccgaactc gccaaacgcg cgaccgaact
     3961 ttttaccagc agccagattt acgatcgcaa cggcaacttg ttgtatgaac tggtcgatcc
     4021 gcagggcggc cggcgcacga ctgtgccgct gaataaaatt tcgccgtggc tgaccaaagc
     4081 gacgatcgat acggaagacc cgcgctttta ttcgcatccc ggtttcgatc cgatcggcat
     4141 catccgcgcg gttttccaga atgcccgatc gggtacggag gcatccggcg catcgacgat
     4201 cgcgcagcaa ttagtccgca acctgttatt gccggaaggc gcggagcgca cgctcaatcg
     4261 caaaatcagg gaagccatct tagcggctga aatcacccgc caatatccgc gcgatacgat
     4321 cttggagtta tatctcaatt cgatcaacta cggcaacctg gcgtatggca ttcaagctgc
     4381 ggccgaaact tacttccata aagatgccgc agccctaact ttagcggaag cctctttctt
     4441 agcgggcatt ccacaagccc cggcggttta tgatcccttc acgaccgatg gtttgaagta
     4501 cacgctcaac cgacaaaagg acgtgctgcg cttgatgcag caagcgggcg acctcactgc
     4561 caatgaaacg aagatcgctt cggatgcgat gcagaacttt gtgttcacgc cgccttcgaa
     4621 cacttttgcg acatccgcgc cgcattgggt cgtgtatgtg aagcagctgg tcgaagaaga
     4681 gttcgggccg gaagcgctat atcgcggcgg cctgaaaatt tacaccacgc tcgatccgaa
     4741 gctgcaatca ctcgcggaac aagtcgtcga cgatcaaatt gcgcaattag gcgataagca
     4801 tgtgaccgat gccggtctcg tcgccatgga tccgcgcacg ggtgaaattc tggcgatggt
     4861 cggcagtccc gatttcaaca acgtggcgat cagcggccag gtcaacatga cgctgcgtac
     4921 gcgccaaacg ggcagcgtca tcaagccgat cacttatttg accgcattcg aaaaaggctg
     4981 gacaccatcg acggtgttat gggatgtccc tgttgtttat acagacacag ccggaaatat
     5041 ttatacgccg ggcaattatg acggccgctt ccacggtccg cagtgggtac gctctacatt
     5101 agcgaactcg tataacattc ccgcggtgaa aactttagtc aacgtgggca tcccgcaatt
     5161 cttggatgtc gctcgacgct tgggtatcga tacattgacg cggcccgatt atggtccggc
     5221 gctgacactc ggcggcggcg aagtgccgct gatcgaaatg accggcgcca ttgcggtgct
     5281 ggcgaatcaa ggcgtatacc tgccgcccat tgcgctcgat cgggtggaga aggccgacgg
     5341 atcgatcgtg tgcaagttca cgccgcccgg tcaagataat caaaatgtgc cgctctgcca
     5401 gccgatcgaa aataccggcg cgcagttggt caagccgcag caggcttatc tgctgacgaa
     5461 tatcctttcg gataacgccg cgcgcacacc ggctttcggc ccgaacagcg cgttagtgtt
     5521 atcgcgtccg gcggccgtca agaccggcac gacgaacgat ttcaaagaca actggacgat
     5581 cggctacacg cctgatctga tcgcgggcat ctgggtcggc aatgcggata acacgccgat
     5641 ggaacacatc agcggtgtga ccggggccgg accgatctgg cacaacttca tggaacaagc
     5701 tttagccgat cggcctgtgc tcgattttgc gcgcccatcc ggcatcgtcg aaatggaagt
     5761 ctgcgccgat tcgggcatac agccatcgca atattgtccc agcaggaagc gagaaattta
     5821 tttcgcggat caattgccgc ccggcccgga tcacgattgg tatcagctgc tgaattgcga
     5881 cggcaatcaa caagtgatgt tggttgtgcc ggatgaagcg cgcgagtggg tcaatcagaa
     5941 tcccgattgg ttagcgagca agggcgtgcc actggcgcca atcggcaatt gcgcgcccgg
     6001 tcagccaaat ggcaacgcga cggtcaacat cacttcaccg gccgatggca gcaccgtcaa
     6061 tggcgtgatc tcgatcatcg gcagtgtgac gctggacaac ttcgatcatt atcagtttga
     6121 gcagggcgtg agcttcgatc ccggcgggtg ggagtgggtc agcggtccgt atcaagcgca
     6181 agtgacgaac ggcgaactcg cacagtggga tacgcgctcg gttaacgatg gcgaacatac
     6241 tttgcgcctg actgcgtttg gcaaaggcgg aggcaagacg gaatatcgat tgcatgtgat
     6301 cgttcagaac aacgggccga cacctacagc aactgataca ccgccaccga ccactacacc
     6361 ggggccgacg ctgaccgata cgccgttgcc attaccgact ttgccgccga gcacagccac
     6421 gccaacgccg acagccacat cacctatacc aactgataca ccgacgaaca ttccattgcc
     6481 gaccgatacg ccgacacttg ctccgtcgcc aacagataca ccaacaccga cggctacgac
     6541 tgaaactcca ccaaccgaaa caccgactga agtaccgacc gtctcgccat agaacacgcg
     6601 agacgttcca gcggaacgcc tctgcaagaa gacaacgggc gatctcatta tagagatcgc
     6661 ccgttttatt agtggggctt acgtttgtca tgctgagcga agcgaagcat ctcggaaaat
     6721 tgtgcgacac ccttcgctca gggtgacaac cttgaatccg ccttgaagcg ataaccctaa
     6781 ttttgtttcg tagagatgat ttgccgagtc gtctctacga aatcgatcgt tgcataatcc
     6841 tcaccggtac accgatcgag cggtagccga aacgttgata caatttttgc gagggcagat
     6901 tgttgccctg tgtgttgacg gtgagcggat acgcgccggc ggcaacttga tagcgcagcg
     6961 attcgatcaa caagcgttcg ccaatgccgc gctgttgaaa gtctggatgc accgccagtc
     7021 gaatgaggtg accctggccg ccatacatgt cgctgaaggc gtgaccgacg acttgttcgt
     7081 cgacttcggc gacgatgaag tgtgcgacga tcggcagcag gtgcgcgatc gatttttcgg
     7141 aataccacca gactgaatca aacgcggcgc gatcattccg ggcgatcgct cgaatgtcgt
     7201 tcgatcgggc cggacgtgta gccacgtcgg gcgttgaagg ggccgccgag ccgggaatcg
     7261 atcggtcatc tttgcgaaga gagatgacat tcacgacggg cgtgaatttt gcagccgtga
     7321 ggatagcggc ataccaatcg ggaaatgcga tcgagacgat ggaggacaca ccctgcgcgc
     7381 gcaagtgcga cgtcgctcga tcgatcaatg cgtcaaagat cgattgcggt tcgtagctat
     7441 cggcgactgc agcactccga atccaggcga cgggtgaatc gtcgatcggg atggctgcaa
     7501 cgccgatcgg ttgggtgtca tgctgcgcga tccagatcgc gtcagaggat cgatcgggat
     7561 acagccaatc ctcaaacgtc caccaatcga gattgaaatg agtgtggcga ttgttttcgg
     7621 tcaggtcgcc gatcgctact tggtcggatg actgcgcggg gcgaacttcg atctcatcgg
     7681 cgcgcaagcg tacggggcgc atgaaacgtt gcatgcgatc atcatatcat cggtaagaga
     7741 agttgccaac cgatgaaacg caaaggcatt atcaggatag aagaagatac ttgcaacgcg
     7801 ctgatgaatg ttgtacactc aacacgctac attagatgac attgacaagg agacgcacat
     7861 gccagccgac ggaaaaaaca aagctttaga aacgacgctc gcttcgatta cgaaaaaata
     7921 tggtgaaggc gccatcatgc gtttgggcga agcgacgcat ctggaaatcg agtcgatccc
     7981 gacgggcacc ttggcgctgg atttggcgct gggcgtgggc ggcattccga aaggccgcat
     8041 cacggaaatt tacggaccgg agtcgtcggg caagacgacg ttgtgccagc acattgtcgc
     8101 ggagtcacag cggcgcggcg gcacgtgcgc ctacattgat atggagcacg cgctcgatcc
     8161 caactacgcc gctatctgcg gcgtcgatat tgagaacttg tatatctcgc agcctgatac
     8221 cggcgagcaa gcgttggaga ttgccgatgc gttgatccat tccggtgcga tcgatgtgat
     8281 tgtgatcgat tcggtggcgg cgctggtgcc gcgcgcggag atcgaaggcg agatgggcga
     8341 cagtcacatg ggtctgcaag cccgcttgat gtcgcaagcc ctgcggaaac tttccggcgc
     8401 gatcaaggcc agcaatgccg ccgtgatttt cacaaaccaa ctgcggatga agatcggcat
     8461 catgtttggc aatcctgaga cgacaacggg cggcaacgcg ttgaagttct acgcttcaat
     8521 tcgattggac gtgcggcgca ttcaaacgat caagggtgat ggcgaggcga cgggcaatcg
     8581 cgtgcgcgtg cgtgtgacga agaacaaagt cgcccccccc ttccgcgaag ccgagttcga
     8641 catcatgttt aatgaaggca tcagcaaagc gggcgacatt ctcgacattg gtacggagtt
     8701 ggagattatc accaaacgcg gcgcgttctt cgcctacggt gataccaaac tcgggcaagg
     8761 ccgcgagaat gcgaaagaat ttttgaaggc gcatgttgaa gtcatgactc agatcgatca
     8821 agagatccgc gcgcatgcgg cgaaggccgc cggcaagaca cggctcggcg gcagcgggac
     8881 agaggccgag gttgcatcgg ataagagcga ggatgaagaa taaagaataa tagaagaagg
     8941 tcggcatagc gccggccttc ttgttta
//
LOCUS       DASRTJ010000198         5006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_11535,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000198 DASRTJ010000000
VERSION     DASRTJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11535"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFF70_04185"
     CDS             <1..1532
                     /locus_tag="VFF70_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43928.1"
                     /translation="FEQAGDDDRTLKYFTLAGDVAARIYANEEALQHYARALIAAKRS
                     HATTAAMHLYAQRGRVLELSGRFSDALANYDEMESHAIEHGDRSMKLASLIARGTLRS
                     TPTPVADSAIGQTLSEQALSLARELGDRKAEATILRNLMLLNNFAAHLNEAVEYGEQS
                     LAIARDLGLREQIAYTLNDLYRPYASIGEYERARTVAEEAREFWRETSNFPMLADNLG
                     RSARILVALGEFDRALVFAEQAHQISHRIGNLWGQSFCRMFVGYVYLERGEMSTGIQT
                     MEECIRLGDEAGFMMPQVATRADLGWIYGTLGAVDHGLELARVARQRAEQRVPTFRTW
                     ALACLARLYVMSDQLAEAETMVKEGYATFTEDFAQHAPFEMPLAEAELALAKQDDARA
                     VSVIDGLLVRLDKFKIHSFRSDALHLKGKALIQLDRIAEARSVLHTARAEAEALGSRR
                     MLWQILAALSRIEIEHRETLRGQAREIVAYIAEHCPAEVRASFLNLPDVRALRTTHEP
                     E"
     gene            1519..3294
                     /locus_tag="VFF70_04190"
     CDS             1519..3294
                     /locus_tag="VFF70_04190"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY43929.1"
                     /translation="MSLNNQLNALESTGLIRLLSAQPSIDYLFRHALIQEAAYRSLTK
                     GDRTELHRVVGTTIEQLYPDRLSAPDLAARLAQHFDHAGDADRALHYYTLAGDAAYQS
                     FANLEAIEHYTHAIKIAARTPVLTDSAQLIQIYTRRGRALEQSGQYERARDNYAAMGQ
                     VAHQRSDRSMELAALIANTTIHVAPTSLRNAEVGQALSTRALALAHELNDRAALAKIY
                     WNLVLLHRHLGNMDQSLVFGEQSIALARELNLREQLAVSLNDILPSYWFTQQNDKAAA
                     ALDEAAQIFRADNNLPMLADNLSTQAMFNVMRGNYESALAVSDEAYQLSKSIGNLWNQ
                     SYSRYLIGYVYLERGEPDQAIEIMTKTIELGAQAGFAFALVQTQTDLGFTYALLGDYA
                     SAEDCARRALADTERLLPDSVSYPFGLLAYVSFQNGQIVEAQQYLDKAKVSAELSEAN
                     IIGFLAWLADFEISMANHTYDHVLESADQALGMMNSLGFRPFRSDVLRFKGQALLAQN
                     KIDEAHELFQQARQIAEELGSRRSLWLALIALSELEAQRGHADQAKALRAEAREIVEY
                     FLDHISDPKLRKSFLNLPMVRATGQ"
     gene            3395..3910
                     /locus_tag="VFF70_04195"
     CDS             3395..3910
                     /locus_tag="VFF70_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY43930.1"
                     /translation="MNPIESLHEGLQWANGLLEMVMADVTPDQLHWLPPGNAHPIGST
                     YAHGVIGEDMLVHNLLQGKTPLFMSSWSGQTGVSEPSFHQTLEWTRSVKIDLPQLRKY
                     AQAVYAAAEAYIDSLSEQDLDRKIELSDSGLGEHTLNWCIQALIIAHLNNMSGEISAA
                     KGMQGAQGYPF"
     gene            3998..>5006
                     /locus_tag="VFF70_04200"
     CDS             3998..>5006
                     /locus_tag="VFF70_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43931.1"
                     /translation="MTTTSTTFTPPYPPSWVDRFIGWIDRLPVPAWLFYIVLLVAEFA
                     ALNILLWTIGTLPTGSFDLARTYSIVYSVGSIAWIHYLNRIASRAIKQFAPALGNDQN
                     EYEAFRYRLTTLPKRTALIVTILNFILEIFSNLLIFAKGGSPAEVFTNLLISMISYGL
                     TGVALYAIVRQLLLVSQIHAVASRVNLFERAPLYAFSGLTVRTGIASLIATYFAIAIN
                     PEAQISVGAILYNSVGLVLAAACFALPLNGMHQRMTKEKARLLARVDRRFETAFDKLH
                     YGFDTEDFSQTDALNKSLTGLTIERDTIQKISTWPWQTGTFRGFSGALILPIIIWLIT
                     RL"
BASE COUNT         1140 a         1387 c         1389 g         1090 t
ORIGIN      
        1 attttgaaca ggccggtgat gacgatcgaa cactgaagta tttcacactg gccggcgatg
       61 tggccgcgcg tatatacgca aacgaagagg ctttgcaaca ttatgcacgc gccctgattg
      121 cggccaagcg cagccacgcg acgacagctg ctatgcacct ctatgctcag cgtggacgcg
      181 tgcttgaatt gagcgggcgg tttagcgacg cgctggctaa ttatgatgaa atggagtcgc
      241 acgcgattga gcacggcgat cggtcaatga aactggcatc gctcatcgcg cgcggcacgt
      301 tgcgtagcac acccacgccc gtcgcggatt cggctattgg gcagactctc tcggaacagg
      361 cgcttagtct agcgcgtgaa ttgggagacc ggaaagccga agcgaccatc ctgcgaaact
      421 tgatgctcct caataatttc gccgctcact taaacgaagc agtcgaatac ggtgaacaat
      481 cactggctat cgcccgcgat ctgggtctgc gagagcagat cgcgtatacg ctaaacgatc
      541 tatatcgacc gtatgccagc atcggcgaat acgaacgcgc gcggactgtc gcggaagaag
      601 cgcgcgaatt ttggcgcgag actagcaact ttcccatgct ggccgacaac ctcggtcgat
      661 ccgcacgcat cctggttgca ctgggtgaat ttgatcgcgc gctggttttt gcggagcagg
      721 ctcaccagat cagtcataga attggcaacc tgtggggtca atcattctgc cggatgttcg
      781 tcggctacgt ttatcttgaa cggggtgaga tgagcacggg cattcaaacg atggaagagt
      841 gcatccggct gggcgatgag gccgggttta tgatgccgca agtggcgaca cgcgccgacc
      901 tcggctggat atatggcacc ttgggcgcag tcgatcacgg gctcgaactg gcgcgcgtgg
      961 cgcgtcagcg ggcggagcag cgggtgccga cgtttcggac ttgggcgttg gcctgtctgg
     1021 cccgattata tgtgatgagc gaccaactcg ctgaagcaga gacaatggtc aaagaaggat
     1081 acgcaacttt cacggaagac ttcgcgcagc acgcgccgtt tgaaatgccg ctggccgagg
     1141 ccgaactagc gctggccaag caagatgatg cacgtgccgt cagtgtgatc gatggcttgc
     1201 tcgtgcgtct cgataaattt aaaatccatt cctttcgatc cgatgcgctg catctcaaag
     1261 gcaaagcgct catccaactc gatcggattg ctgaagcccg atcggtgttg cacaccgcgc
     1321 gcgccgaagc cgaagcgctg ggctcacgtc gcatgttgtg gcagattctg gccgcgttaa
     1381 gccgaatcga aatcgaacac cgcgaaacat tgcgcggaca agcgcgcgaa attgtggcct
     1441 acatcgccga gcattgtccg gctgaagtgc gtgcctcctt cttgaattta ccggacgttc
     1501 gcgctttacg gacaacccat gagcctgaat aatcagctta acgcgcttga atcaacgggc
     1561 ctcatccgtc tgctcagtgc gcagcccagc atcgattatc ttttccggca cgcgttgatt
     1621 caagaagccg cctatcgatc tttgaccaaa ggcgatcgaa ccgagctgca tcgcgtggtc
     1681 ggtacaacga tcgagcagct gtatcccgat cggctgagcg cgcccgacct ggccgcgcgc
     1741 ctcgcccagc acttcgatca tgccggcgat gcggatcgcg cgctgcacta ttacaccttg
     1801 gctggcgatg ccgcgtatca atccttcgcc aacttggaag cgatcgagca ttacacacat
     1861 gcgatcaaga tcgcagcgcg cacgcctgta ttaacagaca gtgctcaact gatccaaata
     1921 tatacacgac gaggccgcgc gctggaacag agcggccaat acgaacgcgc ccgcgacaat
     1981 tacgcggcga tgggacaggt agcgcaccaa cgtagtgatc gatcaatgga gttggccgcg
     2041 ctgatcgcca acaccaccat tcacgtcgcg ccgaccagtc tgcgtaacgc cgaggtcggt
     2101 caagcgttgt ctacgcgagc tttggcactg gcgcacgaac tgaacgatcg ggcggcgctg
     2161 gcgaaaatat attggaattt ggtgttgctg catcgccacc tgggcaacat ggatcaatcg
     2221 ctggtgttcg gcgaacaatc gatcgcgtta gcgcgcgaac tcaatttgcg cgaacagctg
     2281 gccgtaagct taaatgatat tttgccgtcc tattggttca cgcagcagaa tgataaggcc
     2341 gctgccgcgc tggacgaagc tgctcaaatt tttcgcgcgg acaacaatct gcccatgctg
     2401 gccgacaacc tgtccaccca ggcgatgttt aacgtgatgc gcggcaacta tgagtccgcg
     2461 ctggccgtgt cggacgaggc ttatcagctg agtaaatcga tcgggaattt gtggaatcaa
     2521 tcgtacagcc gctatttgat cggctacgtc taccttgagc gcggcgagcc ggatcaagcg
     2581 atcgagatca tgaccaagac gatcgagtta ggcgcgcagg cgggtttcgc ttttgcgctg
     2641 gttcaaactc aaaccgatct tggtttcacc tacgccctgt taggcgatta tgccagcgct
     2701 gaagactgcg cacgacgcgc cttagccgat accgagcggc tgctgcctga ttcggtgagt
     2761 tatcccttcg gcttgctggc ttacgtgagt tttcaaaatg gacagatcgt tgaagcacag
     2821 cagtatcttg acaaagccaa ggtgtccgct gaactgagcg aagccaacat catcggcttc
     2881 ctggcatggc tggctgattt tgaaatttcc atggcgaacc acacctacga tcacgtgctt
     2941 gaatctgccg atcaagcgtt gggcatgatg aacagcctgg gctttcgccc gttccgatcg
     3001 gatgtgctgc gcttcaaagg ccaggcgctg ctcgcgcaaa acaagatcga tgaggctcat
     3061 gagctcttcc aacaagcgag gcagatcgct gaggaactcg gctcgcgtcg atcgttgtgg
     3121 ctggccttga tcgccttgag cgaactggaa gcgcagcgcg gccacgccga tcaagcaaaa
     3181 gcgctacgcg cggaagcgcg cgagattgtc gaatactttt tagatcacat cagcgatcca
     3241 aaattacgca aatcgttttt gaatttgccg atggtgcgag cgacgggaca atgacgttaa
     3301 gataaaaagt ttgcgatcca cgaatctaca ccaatcaaca cgaagaacaa tgaccaaact
     3361 cgtggacaat ctttccgttc acaaggagaa aaacatgaac ccgatcgaat cattacatga
     3421 aggcttgcag tgggcgaatg gcttgttgga aatggtcatg gccgacgtga cgcccgatca
     3481 gctgcactgg ctgccgccgg gcaacgcgca tcccatcgga tcgacctatg cgcatggcgt
     3541 catcggcgaa gatatgctcg tgcacaattt gctgcaaggc aaaacgccgc tgttcatgtc
     3601 gagctggtcg ggtcagacgg gtgtcagcga accctccttt catcaaacgt tggaatggac
     3661 acgctcggtc aagatcgatc tgccgcagct gcgcaagtac gctcaagcgg tgtatgccgc
     3721 cgccgaagcc tacatcgatt cgttaagcga acaagattta gatcgcaaga tcgagctctc
     3781 cgattccggg ttgggcgaac acactttgaa ctggtgcatc caagcgttga tcatcgctca
     3841 cttgaataat atgagcggcg aaatttcggc ggccaagggc atgcaaggcg cacaaggcta
     3901 tccgttctaa gaaatacctt gcgagggttg aaagtgagaa tcgtcaatcg acattgaacc
     3961 cctcgcaagg ttgatctaca aataaaatgg agtcagcatg acaacaacta gcaccacctt
     4021 caccccgcct tatccgccga gctgggtcga tcggttcatc ggttggatcg atcggctgcc
     4081 cgtgcctgcc tggctgtttt acatcgtcct gctggtcgct gaatttgccg cgctgaatat
     4141 cctgctctgg acgatcggca cactgccaac gggcagcttc gatcttgcgc ggacctatag
     4201 catcgtctac agcgttggat cgatcgcctg gattcattat ttgaaccgca ttgccagccg
     4261 cgccatcaag cagtttgcgc ctgcgctggg caatgatcaa aacgaatatg aggcttttag
     4321 atatcggttg acaaccctcc ctaagcgcac agcactgatc gtcaccattt tgaacttcat
     4381 tctcgagatt ttcagcaacc tgctcatttt cgccaaaggc ggatcgccgg ctgaagtatt
     4441 cacaaattta ctcatctcca tgattagtta cggtctgacg ggcgtggccc tatacgccat
     4501 tgtgcggcag cttctcttgg tcagccagat ccatgccgtg gcctcgcgcg tgaatttgtt
     4561 cgagcgcgcg cccttatacg ctttttcagg cttgacggtt cgaactggaa tcgccagtct
     4621 catcgccacg tattttgcaa tcgccattaa cccagaagcg cagatcagtg tgggggcgat
     4681 cctgtacaat agtgtcggcc tggttctggc cgccgcttgt ttcgcgctgc cgctgaatgg
     4741 catgcatcag cgcatgacga aagaaaaagc gcggctgctg gcgagagtcg atcggcgttt
     4801 tgaaacagct ttcgacaagc tccattatgg cttcgacaca gaggacttca gtcagaccga
     4861 tgccttaaat aagagtctca ccggcttgac gatcgagcgc gataccatcc aaaaaatttc
     4921 gacgtggccg tggcagaccg gcaccttccg cggcttctcc ggcgcgctca tcctgccgat
     4981 tattatctgg ctgatcacgc gactgt
//
LOCUS       DASRTJ010000199         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_156362,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000199 DASRTJ010000000
VERSION     DASRTJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156362"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFF70_04205"
     CDS             <1..263
                     /locus_tag="VFF70_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43932.1"
                     /translation="SGWVASPVFDDQHWDDVKRAKGQRAYYILVDFDILLDADRESIL
                     PRAQLNVGVLGSMHWDTQTSGIIIPDKVAKRLEVEWKRLVKK"
     gene            376..1410
                     /gene="tsaD"
                     /locus_tag="VFF70_04210"
     CDS             376..1410
                     /gene="tsaD"
                     /locus_tag="VFF70_04210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZY43933.1"
                     /translation="MTKILAIETSCDETAAAVIQDGRQVLSNIIATQIEMHAKYGGVF
                     PEMASRMHVETIVPVIREALEKAKVNWADLNAIAVTYGPGLPGSLLVGVNVAKGIALG
                     RGLPLIGINHLEGHIYSHWLRVEGDGAAPENLEFPLLILIVSGGHTELILMRDHLKYE
                     KLGATADDASGEAFDKVARLLRLGYPGGPQIEKIAIDGNPKAFKFARPKIDGEFAFSF
                     SGIKTAVLRTVQTYGSIGPDGSISKDVPIADLAASFQQGVIDQLVDKTIGAAEKFGAT
                     SILLSGGVSANKPLRNSMKLKSNVPVFWPPLWLCTDNAAMIGAAAHWRFLAGQRAGWD
                     LDIDPNLKLV"
     gene            1485..>2655
                     /locus_tag="VFF70_04215"
     CDS             1485..>2655
                     /locus_tag="VFF70_04215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZY43934.1"
                     /translation="MTEAVIVDAVRTPVGRHGGMLAVVRPDDLAAIVLKALIDRTKID
                     PAIIEEVYIGCANQAGEDNRNIARMATLLAGFPVEIPAVTLNRLCASSLAAINLAARS
                     IKAGDGDVFIAGGVESMSRAPWSFPKTSSAFPFGNVTAYDTALGWRYPNEKMQALFPL
                     ESMGETAENIFERQPEITREEQDRFSYQSQMKAKAAIESGRFKGEIVPVPIPQRKGDP
                     ILMDTDEHPRPDTTLDALAKLKPAFRKNGTVTAGNSSGLNDGASAVLIMSADRAKSLG
                     LKPMARIIAGAAAGVDPRVMGYGPIPATHKVLKRGGLTIDQIDLVELNEAFAVQSIAC
                     ARELGIPMDKLNVNGGAIALGHPLGCSGARIVTTLVHEMKKRESRYGLATMCVGVG"
BASE COUNT          605 a          640 c          789 g          621 t
ORIGIN      
        1 gatcaggttg ggtcgcatcg ccggtgttcg atgatcagca ttgggatgat gttaaacgtg
       61 ctaaaggaca acgcgcctac tatattttag ttgacttcga tatcctcctc gatgcagacc
      121 gcgaatccat tttgccgcgc gcgcaattga atgtcggtgt tctcggatca atgcattggg
      181 acacacagac ttcggggatt attattcctg ataaagtggc gaagcgattg gaagttgagt
      241 ggaagcggtt agtgaaaaag taaaatgtca ttctgtgtcg cactttgcgc gacgaagaat
      301 ctctaccact gattaacagg gttgttgata gagatagaga ttcctcgctt cgctcggaat
      361 gacaaggtaa ctttgatgac taagatactt gcaattgaaa cctcctgtga cgagacagct
      421 gccgctgtca ttcaggacgg tcgacaggtg ttgagcaaca tcatcgcgac gcagatcgag
      481 atgcatgcca agtatggcgg agtgtttccc gaaatggctt cgcgcatgca cgtcgaaacg
      541 atcgtgccgg tgatcagaga agcgctcgag aaagcgaaag ttaattgggc tgatctcaat
      601 gcgatcgcag tgacgtatgg gccgggcttg cccggatcgt tgttggtcgg agtaaatgtc
      661 gctaaaggaa tcgcgttagg tcgcggcttg ccgctaatcg gcattaatca tcttgaaggt
      721 cacatctatt cccattggct gcgtgttgag ggtgatggcg ccgcaccaga aaatttggag
      781 ttcccgctgt tgatcttgat cgtcagcggc ggccataccg aattgatctt gatgcgcgat
      841 catttgaagt acgaaaaact cggcgccact gctgacgatg cctccggcga agcgttcgat
      901 aaagttgccc gcttgctgcg gcttggctat ccgggcggcc cgcagatcga gaagatcgcg
      961 atcgatggca atccgaaagc gttcaagttc gccaggccca agatcgatgg cgagttcgcg
     1021 ttctcgtttt ccggcatcaa gacggctgtg ctgcgcaccg tgcagactta tgggtcgatc
     1081 ggaccggatg gatcgatctc aaaggatgtg ccgatcgctg atctcgcggc cagttttcaa
     1141 cagggtgtga tcgatcagtt ggttgataaa acgatcgggg ccgccgaaaa atttggagca
     1201 acatcgatct tgttgagcgg cggcgtatcg gctaataagc cgctgcgcaa cagtatgaaa
     1261 ctgaaatcga acgtaccggt gttttggccg ccgttgtggt tatgcaccga caacgcggcg
     1321 atgatcggtg cggcggcgca ctggcgcttc ctggccggac aacgcgctgg ctgggatttg
     1381 gacattgacc ctaacctaaa acttgtttaa cgaaaacgtg ttgcgtggtg cgtattccgt
     1441 tacgcaacac gggttagaca tgaatcttgt gaggtaaaac tactatgact gaagcagtta
     1501 ttgttgatgc agtccgtact cctgtcggcc ggcatggcgg tatgctcgcc gttgttcgac
     1561 cggacgacct ggccgcgatc gtgttgaaag cgctgatcga tcggacgaag atcgatccgg
     1621 cgatcatcga agaagtgtat atcggctgcg cgaatcaagc cggcgaggac aatcgcaaca
     1681 tcgcgcggat ggcgactttg cttgccggat ttccagtcga aattccagcg gtgacgttga
     1741 atcgattgtg cgcttcatcg ctcgcggcga tcaacttagc cgcacgttcg atcaaggccg
     1801 gtgatggcga tgtgttcatt gcgggcggcg tcgaaagcat gagccgcgcg ccgtggtcgt
     1861 ttccgaaaac atcatctgca tttcccttcg gtaatgtgac cgcgtatgat acggcgttag
     1921 gttggcgtta tccaaacgaa aaaatgcaag cgctgtttcc gctcgaatcg atgggcgaga
     1981 cggccgagaa tattttcgag cggcaaccag aaatcacgcg tgaagagcaa gatcgatttt
     2041 cgtatcagag tcagatgaaa gccaaagctg ctatcgagtc tggtagattc aaaggagaga
     2101 tcgtacccgt gccgattcca cagcgcaaag gcgatccgat cttgatggac accgatgagc
     2161 atccgcgtcc cgatacgacg cttgatgctc tggctaagtt aaagcccgcc tttcgcaaga
     2221 atggtacggt gactgccggt aattcatcgg gcttgaatga tggcgcatcg gcagtgttga
     2281 tcatgagcgc cgatcgagcg aagtcgcttg gcttgaaacc aatggcgcgt atcattgcgg
     2341 gtgcggccgc gggtgtcgat ccgcgcgtca tgggctatgg cccgattccc gcaacacata
     2401 aagtgctgaa gcgcggcggg ctaacgatcg atcagatcga tctggttgaa ttgaatgaag
     2461 ccttcgccgt gcaatcgatc gcttgtgcgc gcgaactcgg catcccgatg gacaaactca
     2521 atgtcaacgg cggcgcgatc gccctgggtc atccactcgg ctgttcgggc gcgcgcattg
     2581 tgacaacatt ggtgcatgag atgaagaagc gcgaatcgcg ttatggtctg gctacgatgt
     2641 gcgtcggtgt agggc
//
LOCUS       DASRTJ010000200         5359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_345532,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000200 DASRTJ010000000
VERSION     DASRTJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345532"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFF70_04220"
     CDS             <1..494
                     /locus_tag="VFF70_04220"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HZY43935.1"
                     /translation="HADITTLDVDAIVNAANSTLLGGGGVDGAIHHAAGPQLLEECRT
                     LGGCDTGQAKITLGYRLNARHVIHTVGPIFSGQPLDAELLTSAVKNCLRLADEHRLAS
                     IAFPAISTGVYRYPMKEAAQVILSAIIEYLNSTTAIERVIVCLYDQRALTLFSEMLNQ
                     TAR"
     gene            600..1532
                     /locus_tag="VFF70_04225"
     CDS             600..1532
                     /locus_tag="VFF70_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZY43936.1"
                     /translation="MRVLITGGAGFLGSHLCDKFLAEGHSVIAMDNLITGNTRNIEHL
                     AGNDRFLFIKHDVTNFIYVEGPIDAVLHFASPASPIDYLNLPIQTLKVGALGTHKALG
                     VAKAKGARFLIASTSEVYGDPQVHPQTEDYWGHVNPIGPRGVYDEAKRFAEAMTMAYH
                     RYHSVDTKIVRIFNTYGPRMRLEDGRVVPNFIPQSLRGQPLTVYGDGKQTRSFCYCSD
                     LIEGIYRLLMSAEHDPVNIGNPNELSILEFAQTINRLTGNQAGIIFENKRILDDPQTR
                     RPDITKARTLLNWEPKVALEEGLTKTIEYFRNRV"
     gene            1529..3085
                     /locus_tag="VFF70_04230"
     CDS             1529..3085
                     /locus_tag="VFF70_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY43937.1"
                     /translation="MIQHAPRIMHSLRFTLQQHILNITADQYALSLAYFALAIFGSLT
                     IAIIPLPTLIIGAIVSAILIGTLIEPGIGFIGALLIGPWAAWMNTYYPGLLPIDAGQI
                     LITLTLSAWLLRGLRQRNFTLHRSSILIPLLLYVGWAASSMLWASDLSLAVPEVIKWI
                     EIIVIMLFTIDLIQRLGIKWILIGVMLTVAAQALIGIYEARVRGVGPLGFQLSAGVYR
                     AYGTFEQPNPFAGFIGLVLPIAVALTFYYVRRTLSSTPRSAHHALRIKHYAFLMIYAL
                     ITLLLIAAIYLSFSRGAWLAAVASIGIMIVFAPRRIWIGLGLVVITIGLLIGLSSSGW
                     LPDAINQRFTDASELFQFRDVRGVSINNVNYALIERQAHWQAALNMLTDQPWTGVGFA
                     NYAAAYEQYRLINWPYALGHAHNIYLNVAAETGVIGLAAYLVLWLSIFTVTFRTVQRA
                     SGIDRALAIGLMGTWVYLSVHMLVDNLYVNNAHLLIGALFGLLAFLYSRTRSNLQFPG
                     SNFHSLTSSL"
     gene            3089..3568
                     /locus_tag="VFF70_04235"
     CDS             3089..3568
                     /locus_tag="VFF70_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZY43938.1"
                     /translation="MSRIVTLAKRNKKEVKRFGKFMIIGTIGAVIDGGVLNILAHVFR
                     VDLLIAATISFTMAVSQNFLWNRFWTYPESKGHPFLSQYIQFFAINATALLIRLPILK
                     IAPPPLTTLIRSILQTSPSTTEVLANNGAWAIAVGIAMFWNFFINRFVTYRHIKVGQ"
     gene            3597..4112
                     /locus_tag="VFF70_04240"
     CDS             3597..4112
                     /locus_tag="VFF70_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZY43939.1"
                     /translation="MSRLITLAKTNRKEFKRFAKFLVVGIVGFVVDFGTFNLLLRALN
                     FNPIVAQSISFTCAAISNFTWNRIWTYPESRSKPILLQFGQFFILNLIGVLVRSVVFG
                     LLSAPMIGLVTSMQSGPFAGLIGFATNTLHMTIEQLGYNGALAIAVVVVLFWNFFSNR
                     FITYGDVKIGH"
     gene            4205..5344
                     /locus_tag="VFF70_04245"
     CDS             4205..5344
                     /locus_tag="VFF70_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY43940.1"
                     /translation="MKIGIDYTSAVSQGAGIGRYTRELIRAVLALPSDHYFSLFYASR
                     TNIQESEIRNQRSEIHHLRFHDKWLTRIWHRARLPIPIEVVIGKIDLFHSPDFTLPPT
                     MPGVPTLLTVHDLSFMRDPDSAWPSLRAYLMQAVPRSVKRATHLLADSQATKDDLIDL
                     FKTPPEKITVLYSGVDARFTPIRDRAEIDRVREKYKIGPSPFALSVGTLQPRKNYRRL
                     IEAYAKLNNHRLHLVIGGGRGWMFDDLFTLVQSLQLEDRVHFIGFVKDEDLSTLYSAA
                     DLFVYVSLYEGFGLPLLEAMACGTPVIGSNTSSLPEVIGDVGLQVDPKDVDAIAQAML
                     QMLTRSDWRDRSVAAGIDRAKLFTWDKAAQQLLSIYEQLSYRKRE"
BASE COUNT         1199 a         1461 c         1353 g         1346 t
ORIGIN      
        1 ttcacgccga tatcacgacc ctcgacgtcg atgcgatcgt caatgcagct aacagtacat
       61 tgctcggtgg tggcggtgtc gatggcgcga tccatcacgc cgccggaccg cagctcctcg
      121 aagaatgtcg cacattgggc ggctgcgaca caggtcaagc gaagatcacg ctaggctatc
      181 gattaaacgc gcggcacgtg attcacactg ttggcccgat cttttctgga cagccactcg
      241 atgcagaatt gctgacctct gccgtgaaaa attgtttgcg cttggccgat gagcaccgcc
      301 tcgcctcgat cgcgtttccg gcgatctcga ccggtgtata tagatatcca atgaaagaag
      361 cggcgcaggt gattctgtcg gcgatcattg aatacttgaa cagcactaca gcgatcgagc
      421 gcgtgatcgt ctgtctgtac gatcagcgcg cactcaccct attcagtgaa atgctgaatc
      481 agacggcgag ataacgagat agcccttcac tctatctcgc aagcccgcta tctcgccgtc
      541 tctgtatcaa tcccctgtgg taaaatggcg cagcacctca tccctcaccc ggagttaaca
      601 tgcgcgtact catcacaggc ggcgcgggct ttctcggctc gcacttgtgc gataaatttt
      661 tggctgaagg tcacagcgtc attgccatgg acaatctgat caccggcaac acccgcaaca
      721 tcgaacactt ggccggcaac gatcgcttct tattcatcaa gcacgatgtc accaacttca
      781 tctacgttga aggcccgatc gatgcggtgc tgcatttcgc gtcaccggcc agcccgatcg
      841 attatttgaa cttaccgatc caaacgttga aggtcggcgc gttgggcacc cacaaagcat
      901 tgggcgtcgc caaagcgaaa ggcgcgcgat ttttgatcgc ctccaccagc gaggtgtatg
      961 gcgatccgca agttcatccg cagacggaag attattgggg ccacgttaat ccgatcggac
     1021 cgcgcggcgt gtacgatgaa gccaaacgat tcgccgaggc gatgaccatg gcctatcatc
     1081 gttatcacag cgtcgatacg aagatcgtgc gcatcttcaa tacctacggc ccgcgcatgc
     1141 ggctggaaga cggccgcgtc gtgccaaact tcattccgca atcactgcgc ggccaaccac
     1201 tgacggtata cggtgacggt aaacagaccc gatcgttttg ctactgctct gatctcatcg
     1261 aaggcattta tcgcttgttg atgagcgcgg agcacgatcc ggtcaacatc ggcaatccca
     1321 acgaactttc gattctcgaa ttcgcccaga ccatcaatcg gctgacgggc aatcaagctg
     1381 gtatcatctt cgagaacaaa cgcattctgg atgatccgca aacccgccgg cctgacatca
     1441 ccaaagccag gacgctgctg aattgggaac cgaaagttgc acttgaagaa ggcttgacga
     1501 aaacgattga gtattttcgg aaccgcgttt gattcagcat gcaccgcgca tcatgcatag
     1561 tttaagattc actcttcagc aacatatcct caacatcact gccgatcagt atgcactcag
     1621 ccttgcgtat tttgccttgg ccatcttcgg ctcgctgacc atcgcgatca ttcccttgcc
     1681 cacgctaatc atcggtgcga tcgtcagtgc gatcctaatc ggcacattga ttgaaccggg
     1741 tatcggcttc atcggcgcat tgttgatcgg cccgtgggct gcatggatga acacgtatta
     1801 tcccggccta ctgccgatcg atgccggaca gatcctgatc acgttgactc ttagcgcgtg
     1861 gttgctgcgt ggtctgcgtc agcgaaactt taccttacac cgatcgtcga tattgatccc
     1921 gttgttgctg tatgtgggtt gggccgcaag cagtatgtta tgggcgtccg atctgtcgct
     1981 ggccgtgccc gaagtgatta agtggatcga gatcatcgtg atcatgctct tcacgatcga
     2041 tctgatccaa cggcttggca tcaaatggat cttaatcggc gtgatgctga ccgtcgccgc
     2101 gcaggccttg atcggaatct acgaagcccg cgtgcgtggt gtcggcccgc tcggtttcca
     2161 gttatccgcc ggcgtttatc gcgcctacgg tacattcgag cagccaaatc cgtttgcggg
     2221 gtttatcggg ttggtgctgc ccatagcggt agcgctgact ttctactatg tgaggcgtac
     2281 cctatcctcc acgccgcgaa gtgcgcatca cgcattacgc attaagcatt atgccttcct
     2341 catgatctac gctctcatca cactcctgct tatcgccgca atctatttat ccttctcacg
     2401 cggagcatgg ttggccgccg tcgcctcgat cgggatcatg atcgtttttg cgccgcgcag
     2461 aatttggatc gggttgggtt tagtcgtcat cacgatcgga ctgctgattg gcctgtcgag
     2521 ttcgggctgg ctgccggacg cgatcaatca acgttttacc gacgcgagcg agttgttcca
     2581 gttccgcgac gtacgcggcg tatcgatcaa taatgtgaac tatgcactga tcgaacgaca
     2641 ggcccattgg caggcggcgc tgaacatgct aaccgatcag ccgtggaccg gtgtggggtt
     2701 cgccaactac gccgcggcgt acgagcaata tcgcttgatc aactggccgt atgctttagg
     2761 ccacgcgcat aacatctatt tgaatgtcgc cgccgagacg ggcgtgatcg ggttagccgc
     2821 ttatttggtc ttgtggcttt caatttttac ggtaactttc agaaccgtcc agcgggccag
     2881 cgggatcgat cgggcgttag cgatcgggct gatgggcacg tgggtctatc tctccgtgca
     2941 catgctcgtc gataatctat atgtcaataa cgcgcacttg ttgatcggcg cgctattcgg
     3001 actattggca tttctgtaca gccgcacccg ttccaatctc caatttccag ggtcaaattt
     3061 ccattctcta acttccagcc tctaacgcat gtcacgaatt gttaccttag ccaaacgcaa
     3121 caagaaagaa gtcaagcgct tcggtaagtt catgatcatc ggcacgatcg gcgcggtgat
     3181 tgatggcggt gtattaaata tactggcaca tgtcttccgt gttgatctgc ttatcgctgc
     3241 cacgatctca tttacgatgg cagtctcaca gaatttttta tggaaccgct tctggactta
     3301 tcccgaatcg aaaggtcatc catttctgag tcaatatatt caattcttcg ccattaacgc
     3361 aactgcgctg ctgattcgct tgcctatctt gaaaatcgca ccgccaccgt tgacaacatt
     3421 gatcagatct attctgcaaa catcaccatc aactacggaa gtgcttgcga acaatggggc
     3481 gtgggcaatc gccgtcggca ttgccatgtt ctggaatttc ttcatcaatc gtttcgtaac
     3541 ctatcggcat attaaagtag gacagtagac caaagtagca gtttgttcca catttcatgt
     3601 cacgactgat cactctcgcc aaaacaaatc gcaaagagtt caaacgcttc gccaaatttc
     3661 tggtcgtggg cattgtcggc ttcgtcgtcg actttggcac attcaatttg ctcttgcgcg
     3721 ctttgaactt taatccgatc gttgcgcaga gcatttcgtt cacctgcgct gcgatcagca
     3781 actttacctg gaatcggatc tggacttatc ctgaatctcg atcgaaacca atcctgctgc
     3841 aattcggcca gttcttcatt cttaacttga tcggtgtgct cgtccgatcg gtggtgtttg
     3901 gtttattgag cgcaccgatg atcggcttag tgacttcgat gcagagcgga ccgttcgctg
     3961 gactgattgg cttcgccacc aacacgctgc acatgacgat tgagcaactt ggctataacg
     4021 gcgcgctggc catcgccgtg gtcgtggtgc tgttctggaa tttcttctct aatcgattca
     4081 ttacttatgg tgatgtaaaa atcggacact gattggagat gagaagttag aaattggaag
     4141 ttggaaagat caatcattga ttaataacac tcctccaacc tccaatgtcc aacatccaac
     4201 ttctatgaag attggcatcg attacacctc agccgtttcg caaggcgcgg gcatcggccg
     4261 ctacacgcgc gagttgatcc gcgccgtgtt agccctgcca tcggatcatt acttctccct
     4321 tttttacgcc agccgcacaa atatccagga atcagagatc agaaatcaga gatcagagat
     4381 tcatcacttg cgatttcacg acaagtggct cacccgcatt tggcatcgcg ctcgattgcc
     4441 gatcccaatc gaagtggtca tcggtaagat cgatctattc cattcgcccg atttcactct
     4501 gcccccgaca atgcccggcg tcccgacgct gctcacggta cacgatttat ctttcatgcg
     4561 cgatccggat tcggcctggc cgagtctgcg cgcgtatctc atgcaggccg tgcctcgatc
     4621 ggtgaagcgg gcgactcacc tactggccga ttctcaggcg accaaagatg atctgatcga
     4681 tctattcaag acaccgccgg aaaaaatcac ggtgctgtat agcggagtgg atgcgcgctt
     4741 cacgccgatt cgtgatcgcg cggagatcga tcgagtcaga gagaaataca agatcggacc
     4801 atcaccgttc gcattatcgg tcggcacctt gcagccgcgc aaaaattatc ggcgcctgat
     4861 cgaagcgtat gcgaagttaa acaaccatcg attgcattta gtcatcggtg gcggcagagg
     4921 gtggatgttc gatgatctct tcacgctcgt ccaatcattg caacttgaag atcgagttca
     4981 cttcatcggt tttgtgaaag atgaagattt gtcaacattg tattccgccg cagacctctt
     5041 tgtctatgtc tctttatatg aaggctttgg tctgccgcta ttagaagcga tggcgtgcgg
     5101 cacgccggta atcggttcaa acacatcttc gctgcctgaa gtgatcggtg acgtcggctt
     5161 gcaggtcgat ccaaaggatg ttgatgcgat cgcgcaggcg atgttgcaga tgctaactcg
     5221 atcggactgg cgcgatcgat cggtggccgc cgggattgat cgggcgaagc tattcacgtg
     5281 ggataaagcc gcgcagcaat tactatcgat ctatgagcaa ctatcatacc gaaagagaga
     5341 atgagggata gagagacat
//
LOCUS       DASRTJ010000201         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_314463,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000201 DASRTJ010000000
VERSION     DASRTJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314463"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFF70_04250"
     CDS             complement(<1..323)
                     /locus_tag="VFF70_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43941.1"
                     /translation="MIRTEELSKVFIGDKKNKIHAVDGLTLAVKQGEVFGFLGPNGAG
                     KTTTVRMLSALIGVTTGRAWVNNFEIGKQDQEVRGSIGILTEQPGMYDRLSAEKNLTI
                     FAKLYG"
     gene            complement(337..1053)
                     /locus_tag="VFF70_04255"
     CDS             complement(337..1053)
                     /locus_tag="VFF70_04255"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZY43942.1"
                     /translation="MLRKWFAITLIAALAGACGTNPMPVATSNELKLSPPWTGTEHFD
                     YTVMADQDGSPIGSGAIDVKPSSDATTIEQHYQFDKVAQHLVVRIDPQSLKPLGGTQQ
                     VSGSPNDYSLATKYQNNELTIKATTSQGEKDFTLEVAPDAIDNDSVLMVLRGVPLTDG
                     YSASFHIVVATTALQVPTTLNVAARETVTVPAGTFNTYKVTLGFGSGPGQTAWYEVAA
                     PHRMIQYDNGQDKFVLVKSS"
     gene            complement(1103..1894)
                     /locus_tag="VFF70_04260"
     CDS             complement(1103..1894)
                     /locus_tag="VFF70_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43943.1"
                     /translation="MQTDAFIGLILICAIIIPAMIYLILRGITPLKQLALKWWWLLYV
                     IAFIAQPIFLLRKHWEGALVFTNPSSLTLQLIAQADNAWWEELAKLLALSLIVWLARD
                     QIKSFFKILSTSINLGLWAGLAYGAGEAVVLGILFAAPNLAPIFGMHTFSPFTLGWAF
                     VLERIWAMQLHAIMGALIGAGVWCWYNGKRGGFIVWFIAAMVYHDFVDGQIILAQFNS
                     AIASFLTQFGIALLLIDVALGYLLIALVYRVHNRSRAARRITPQT"
     gene            complement(1907..2314)
                     /locus_tag="VFF70_04265"
     CDS             complement(1907..2314)
                     /locus_tag="VFF70_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZY43944.1"
                     /translation="MNLKINSRDRTPIYQQIIQGIKHQVAAGALKPGEQIPTIRALAD
                     QLGVNVNTISRAYASLDRSGVITVQQGRGTYITEKPDHAGMREERHAELHRVIGYTVL
                     EALSLGYTHGEIEAAFAENLNHWRREHRTPRKK"
     gene            complement(2468..3514)
                     /locus_tag="VFF70_04270"
     CDS             complement(2468..3514)
                     /locus_tag="VFF70_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZY43945.1"
                     /translation="MHKHFRITTRLLSLAVVFLLAGCAELALPPDQLNAPDPSFDTAV
                     VALNIAPTPTAQPVPIASETPTPSPTPQPSPTPTLFAAAMNVADPNCHESAGQVVKDK
                     FASTVIGRDVPVKLYLPPCYDDAGNIDQYPVIYLFHGSPFNENHWISVGAIDAANNLI
                     GSGELPPFIIVLPRGDLDGTFGHSSGGDNSWEGVVVNELVPYIDGNYRTLTNRHYRAI
                     GGISRGGVWSLEIGLRHPDLFSSIGSHSPALSVNLASGDYDPFVIVRNRPIDSMRIYL
                     DAGDTDWTRPETSNLSKALDDLHLPHTFAINPGDHLDSYWSSQVYAYLRFYAAPWKAE
                     RVAAIQAQTPASPP"
     gene            complement(3619..>4537)
                     /locus_tag="VFF70_04275"
     CDS             complement(3619..>4537)
                     /locus_tag="VFF70_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZY43946.1"
                     /translation="SNGQSLDELESKLAQARRKLCEAREQRVKPNRDEKILSGWNGLM
                     LAAFAEAARDRSLDRSDQYRLIAEKNAAFLLRSMRTPEGRLYRSWKDGQAKLNGYLED
                     YASVIEGLLALYQTTFDEQWFIAARELADVMIKHFADPAGGFFDTSDDHETLVTRPKD
                     LQDNATPSGNALAATVLLKLHLYTMEDLYYKQAELMLGLLQPAMIQAPTGFAQWLCAL
                     DFALSKPFEIAIVGDIRSRDTQALLEAAFKGYRSNQVVAVGAPDRSSVVPLLLDRPQI
                     DHQATAYVCQNFTCQRPAIDPKALIEQLS"
BASE COUNT         1065 a         1211 c         1222 g         1039 t
ORIGIN      
        1 ccatatagct tagcgaagat agtcaaattc ttttcggcgc tcagtcgatc atacatgccg
       61 ggctgctcgg tgagaatccc gatcgagccg cgcacctctt gatcttgttt gccgatttcg
      121 aaattgttta cccacgctcg accagtggtc acgccgatca aggcactgag catccgcacc
      181 gtcgttgtct tgccggcgcc attcggtcca aggaaaccaa acacttcgcc ttgcttaaca
      241 gcaagcgtca gaccatccac cgcgtgaatt ttatttttct tgtcgccaat aaaaacttta
      301 ctgagttctt ccgttcgaat catgctgcct ccggtgctat gaagacttca ctaacacaaa
      361 tttgtcttga ccattatcgt attgaatcat gcgatgcggt gcggccactt cataccaagc
      421 ggtttggccc ggccccgaac cgaaaccgag cgtgactttg taggtattaa acgtaccggc
      481 tggaaccgtg accgtttctc tggcggccac attcaatgtg gttggaactt gcaacgcagt
      541 tgtagcgacc acaatatgaa aagacgccga gtatccatcc gtgagcggca caccccgcaa
      601 gaccatcaat acactgtcat tatcgatcgc gtccggtgca acttcaagcg taaaatcctt
      661 ttcgccttga gacgtcgtcg ctttgatcgt tagttcattg ttttgatatt tcgttgccaa
      721 agagtaatcg ttcggcgatc cactgacttg ctgagtgcca ccgagcggtt tgagcgattg
      781 tggatcgata cggacgacca ggtgctgtgc cactttgtcg aattgatagt gttgctcgat
      841 cgtggtggcg tcgctcgacg gcttcacgtc gatcgcgccg gatccgatcg gcgatccatc
      901 ctgatcggcc atgaccgtgt aatcaaagtg ctcggtgcca gtccacggcg gtgacaactt
      961 caattcgttc gatgtcgcca ctggcatggg atttgtgccg caggccccgg ccagcgcagc
     1021 tatcagcgtg atcgcgaacc atttccttag catattaaag ccattccctt attgatctaa
     1081 ttgaaccaga ccaattagta gattaagtct ggggcgtaat acgtcgagcc gcgcgagatc
     1141 gattgtgcac acgataaacc aaagcaatga gcaaataacc aagcgctaca tcgatcagta
     1201 acagggcaat tccgaattga gtcaaaaatg acgcaatcgc ggaattgaac tgagccaaaa
     1261 tgatctgccc atcgacaaaa tcgtgataca ccatcgcggc gatgaaccac acgatgaagc
     1321 cgccgcgttt gccgttgtac caacaccaca cgcctgctcc gatcagcgcg cccatgatgg
     1381 catgcagttg catcgcccag atgcgctcta acacaaatgc ccagccgagt gtgaacggcg
     1441 agaaagtatg cataccgaag atcggtgcga ggttaggcgc agcgaataaa atgcccaaga
     1501 ccaccgcttc gcctgcgccg taagccaagc ctgcccacaa gccaaggttg atcgaagttg
     1561 ataatatctt gaagaacgat ttaatctgat cgcgcgccaa ccaaacgatg aggctgagcg
     1621 ccagcaactt ggcgagttct tcccaccagg cgttgtccgc ttgcgcgatc aattgaagag
     1681 tcaacgaaga tggattggtg aagaccagcg cgccttccca atgtttgcgc agtaaaaaga
     1741 ttggttgagc gatgaaggcg atgacatata acagccacca ccatttcagc gccaattgct
     1801 tgagtggtgt gatgccgcgc aggatcaaat agatcatcgc cgggataatg atcgcgcaga
     1861 tcaatatcag gccgatgaag gcgtcggttt gcatgtgagg ctcgaattat tttttgcgcg
     1921 gagtgcgatg ctcgcggcgc cagtgattca agttctcggc gaaggcggct tcgatttcgc
     1981 catgcgtgta gccgagactt aacgcttcca gcacggtgta tccgatcacg cgatgcagct
     2041 cggcgtggcg ctcttcacgc atcccggcgt gatcgggttt ttcggtgatg taagtaccgc
     2101 ggccttgctg caccgtaatc acgcccgatc gatcgagcga agcgtaggcg cgggagatgg
     2161 tgttgacatt gacacctaat tgatcggcca aagcccggat ggtgggaatc tgctcgccgg
     2221 gcttcaaggc tccggcggcg acttgatgct tgatgccttg aataatttgt tgatagatcg
     2281 gcgtgcgatc gcgcgaattg attttgaggt tcatgtgatt gtcctaattg atgtgtgaca
     2341 attatatgaa gtcgtgcgaa cttgtcaagc tcgctaaaaa ataaaaaccg aacgggctgt
     2401 tcggtttttc catgaaggtt tgtgatccat ttatttttag tttacgcgat agccctccaa
     2461 caatcgatca cggcggcgag gcaggtgttt gggcttgtat cgccgcgacg cgctccgctt
     2521 tccacggcgc ggcatagaag cgcagatagg catacacttg cgaggaccaa taactgtcca
     2581 agtgatcgcc gggattgatg gcaaaggtgt gcggcaaatg caaatcgtcc agcgccttgc
     2641 tgaggttgct ggtttcgggg cgcgtccaat cagtatcgcc ggcatcgaga tagatgcgca
     2701 tcgaatcgat tgggcggttg cgcacgataa cgaatggatc ataatcgccc gaagcgagat
     2761 tcacgctcag cgccggacta tggctgccga tcgaagaaaa caaatcggga tgacgcagac
     2821 cgatttctaa tgaccacaca ccgccgcgtg aaatgccgcc aatggctcga taatgccgat
     2881 tcgtcagcgt tcgataattg ccatcgatat acggaaccag ttcgttgacg acgacgcctt
     2941 cccacgaatt gtcgccgccg ctcgaatggc cgaaggtgcc atcgagatcg ccgcgcggca
     3001 gaacgatgat aaatggcggc agttcgcccg agccaatcaa attgttggcc gcatcgatcg
     3061 cgccgacgct gatccaatga ttttcgttga agggcgatcc atgaaacaga taaatcaccg
     3121 gatattgatc gatgttgcct gcgtcgtcgt aacacggcgg caaataaagc ttgacgggca
     3181 catcccgccc gatcacggtg ctggcgaatt tatctttgac aacttgcccg gccgattcat
     3241 ggcaattcgg atcggccacg ttcatggctg cggcaaacaa cgtgggcgtc ggcgatggct
     3301 gcggtgtcgg cgagggggtc ggtgtctcag acgcgatggg caccggttga gccgtcggtg
     3361 tcggggcgat gttgagcgcg acaacggccg tatcgaaaga tggatcgggg gcgttgagtt
     3421 gatcgggcgg cagcgccagt tcggcacagc ccgctaataa gaaaacgact gctagtgaga
     3481 gcagtcgcgt ggtgatgcga aaatgtttat gcactgctac ttctattctc tggctaggct
     3541 agacaacggc gaggattcta ccagaagatt ggcaaatcac aaagaggaga ttttgtcggg
     3601 gcgcacggcc atgcgccctt acgacaattg ctcgatcaac gctttcggat cgatcgccgg
     3661 gcgttggcac gtaaaattct ggcagacata cgctgtggct tgatgatcga tctgtggccg
     3721 atcgagtaac agcggcacaa ccgaagatcg atcgggtgcg ccgacagcca cgacttgatt
     3781 cgatcgatag cctttgaagg cagcctcaag caaagcttgt gtgtctctcg atcggatatc
     3841 gccgacgatc gcgatctcaa acggtttgct caacgcgaag tccagcgcac acagccattg
     3901 agcgaaaccg gtcggcgctt gaatcatggc cggttgcagc aagcccagca tcaactcggc
     3961 ctgcttgtaa tacaagtctt ccatcgtata caaatgcagt ttcaacaaaa ccgtcgcggc
     4021 cagcgcattg cccgacggcg tggcattgtc ctgcaaatct tttggacgcg tgaccaatgt
     4081 ttcatgatca tcgctcgtgt cgaagaagcc gccggctgga tcggcgaagt gtttgatcat
     4141 cacatcggcc aactcgcgcg cggcaatgaa ccactgctca tcgaaggttg tttgatacaa
     4201 cgccagcaac ccttcgatca cactcgcgta atcttccaaa taaccgttca acttcgcctg
     4261 cccgtctttc cacgatcgat acaagcgtcc ttcgggcgtg cgcatcgatc ggagcaggaa
     4321 ggccgcattc ttttccgcga tcaatcgata ttgatcggat cgatccagtg atcgatcgcg
     4381 cgccgcttcg gcgaacgcag cgagcatcaa gccgttccaa cccgatagga ttttctcgtc
     4441 ccgattgggc ttcacgcgct gctcacgcgc ttcacacaac ttgcgccggg cttgagctaa
     4501 tttagattcg agttcatcaa gcgactggcc atttgat
//
LOCUS       DASRTJ010000202         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_118517,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000202 DASRTJ010000000
VERSION     DASRTJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118517"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFF70_04280"
     CDS             complement(<1..361)
                     /locus_tag="VFF70_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZY43947.1"
                     /translation="MDAQKITIKNGKLLVPDNPVLPFIEGDGTGPDIWRASVRVLDAA
                     VKKAYGGQRSIVWKEVLAGQKAFDQTNNWLPAETVEAFREYLIGIKGPLTTPIGGGIR
                     SLNVALRQMLDLYVCLRP"
     gene            complement(502..2112)
                     /locus_tag="VFF70_04285"
     CDS             complement(502..2112)
                     /locus_tag="VFF70_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZY43948.1"
                     /translation="MFRFRRTLLWLLIVASFLVTDRPVAQAIPPTDPVPIEPLPDGAV
                     LETFVNSAHNLVAMDFTPDGRWLYTERTSGYDGDVGGNAGYVRVVDHGILQPTPVYKF
                     PVDPNGERGLLGIAVDPNFTSNHRVWTYFTKLNSNGTLNNRVAWLILNNDNSVSANNE
                     GIAAAFPVDYYISVHNGGNLHFGPDGKLYVTVGNNNVTNDNSDPAQSLASPLGKIHRF
                     NPTVPLSTPSDNPFGNNTIYAYGLRNSFDFAFDPISHQLFATENGDACDDEINLILRG
                     GNYGWRPFYPCDDEAPTGPAPQYNTIRPLIYWTPTKAPTGLTFYTGDLFPEWQSDMFM
                     CAFKDSSTALYHFKLNTARTAIASETVISDSTSPNIHCRTDVLTGPDGALYYSQDGGF
                     NDGPIMRITRTSSYIASAIDVTPAAVRAGDIATFTLRARHAGTLSNTFNLTATIPPSM
                     TLLPGAIQYQHGVLNYEADHIWWTGLLSHTETWTATYQVQITAAMTSPAILIGSMDIS
                     APGHPTFSFTTNLLVNGYEIYLPIIMRN"
     gene            complement(2172..>2390)
                     /locus_tag="VFF70_04290"
     CDS             complement(2172..>2390)
                     /locus_tag="VFF70_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY43949.1"
                     /translation="GCIDGIYYALGYGGHGVAMSTYLGAQLAEVISGRPSHNPFEKFS
                     FPAIPLYTGRPWFLPFGAIYYKVMDLIS"
BASE COUNT          579 a          619 c          662 g          530 t
ORIGIN      
        1 ccggccgcaa gcacacatat agatcgagca tctgccggag cgccacgttg agcgatcgga
       61 tgccaccccc gatcggggtg gtcaacggac ctttaatgcc gatcaagtat tcacgaaacg
      121 cctcgacggt ttcagcgggc aaccaattgt tcgtttgatc gaatgctttc tgtcccgcta
      181 acacttcttt ccagacgatc gatcgttggc cgccgtaggc tttcttcacc gcggcatcga
      241 gcacacgcac actcgcacgc caaatatccg gtcccgtccc atcaccttcg ataaacggca
      301 gcaccggatt gtcgggcacg agcaatttcc cgtttttgat tgtgatcttc tgtgcatcca
      361 tgatgaattg ctcctatcgt aaactatcct cccgcaggtc gcttgatgaa gaggaattca
      421 gcattgagac ctgcgggagg atgaatgaag tcgatcggga ttatagcatg aatcatcggg
      481 caggcgcggc gcgatcaatc ctcagtttcg catgatgatc ggtaaataaa tctcgtaacc
      541 gttgacaaga agattcgtcg tgaagctaaa ggtcggatgg ccgggcgcgg agatgtccat
      601 cgagccgatc aagatcgcgg gcgatgtcat cgcggccgtg atctgaactt gataagtggc
      661 tgtccaggtt tcggtatggg atagcagtcc cgtccaccaa atgtgatcgg cctcgtaatt
      721 taatacgccg tgctgatatt gaatggcgcc cggcaacagc gtcatcgagg gcggaatcgt
      781 cgcggtcaaa ttaaaggtgt tcgacaaagt gcccgcatgc cgcgcgcgca acgtaaacgt
      841 ggcgatgtct ccagcgcgca ctgcggcggg agttacatcg atcgctgagg caatataaga
      901 agaggtgcgc gtgatgcgca tgatcggccc atcgttgaag ccgccatctt gcgagtaata
      961 cagcgcgcca tccggcccgg tgagcacgtc ggtgcggcag tgaatattgg gcgaggtact
     1021 atcgctgatt actgtctctg aggcaattgc tgtgcgcgcc gtgttcaact tgaaatgata
     1081 cagcgccgtc gaagaatcct tgaaagcgca catgaacata tcactctgcc attcgggaaa
     1141 cagatcaccg gtatagaacg tgaggccggt cggggctttg gtcggcgtcc aataaatcaa
     1201 cggacggatc gtgttgtatt gaggagcggg gccggttggt gcttcatcgt cgcatggata
     1261 aaatggccgc caaccgtaat tgccaccgcg caaaatgaga ttgatctcat cgtcgcacgc
     1321 atccccattt tcggtcgcaa agagttgatg actgatcgga tcgaatgcaa aatcgaacga
     1381 gttgcgcagg ccataggcat agatcgtatt attgccaaag gggttatcgc ttggcgtgct
     1441 gagcggcact gttggattaa agcgatgaat cttgcctaaa ggcgaggcca aactctgcgc
     1501 tggatcgctg ttgtcattcg tgacattgtt gttgccgacg gtcacgtaca actttccatc
     1561 cggtccaaaa tgcagattgc cgccgttgtg aaccgaaatg taataatcga ccgggaacgc
     1621 tgccgcaatg ccttcattgt tcgctgagac cgagttgtcg ttgttcaaaa taagccaggc
     1681 aactcgattg ttcaacgtgc cgttgctgtt taatttagtg aagtacgtcc acactcgatg
     1741 attcgaagtg aaattcggat cgacggcaat gcccaacagg ccgcgctcgc cattcggatc
     1801 gaccggaaat ttatagacgg gggttggctg caaaattcca tgatcgacca cgcgcacata
     1861 acccgcgtta ccgccgacat cgccgtcgta gccgctggtg cgttcggtat acaaccagcg
     1921 gccatccggc gtgaaatcca tcgccaccaa attatgcgca ctgtttacaa atgtttccaa
     1981 aactgcgccg tcaggcagcg gctcgatcgg aactggatcg gttggcggga tggcttgagc
     2041 gacggggcga tcggtaacga ggaacgaagc cacgatcaac agccatagga gagtgcggcg
     2101 aaaacggaac aaaacgatct gctttgtgat ggccgaattt cgaccggatt atgactaaag
     2161 ccgatcgatg atcaactaat caggtccatc actttatagt atatcgcgcc aaatggcaag
     2221 aaccatggcc gccccgtgta taacggaatc gcggggaagg aaaatttttc gaaggggttg
     2281 tggctggggc gaccactaat cacttcggcc aactgcgcgc cgagataagt agacattgcc
     2341 acgccgtgac cgccgtaacc caacgcataa tagatgccat cgatgcagcc
//
LOCUS       DASRTJ010000203         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_49892,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000203 DASRTJ010000000
VERSION     DASRTJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49892"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VFF70_04295"
     CDS             <1..980
                     /locus_tag="VFF70_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZY43950.1"
                     /translation="ENLIQVLGDEHSQFESPSAVADSKAALAGTINYVGIGVLILPLP
                     DKKRATVLALLPNSSAAHDGRLHPHDAILAVDGLPLVENGVSYPQRVRGPECSAVVLT
                     VQSPGQSPRDITLLRYRVSGAEPIGARLVSTADGARIGYLFLPTFFDETIPAQVKKAL
                     EDFGSLDGLILDNRMNGGGSSDVLNPVLSYFTSGTLGRFVSRTVTRTLSLKADPINTS
                     QTVPLIVLVGEDTVSFGEIFAGILQDVGRAKIVGQTTLGNVETLHGYDFQDGSRLWIA
                     QERFDPEQSHANWEQTGIIPDMPAFADWDTFTFENDPSIAAALKLLGHE"
     gene            complement(1057..1932)
                     /locus_tag="VFF70_04300"
     CDS             complement(1057..1932)
                     /locus_tag="VFF70_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZY43951.1"
                     /translation="MADEEQSEEEVRPAWSSQFSPDVLKAISSLGPMGEITREWAWGG
                     STGKGAKVAIVDSGIDHDHPAVGDAVKGGIAIEYDGRLKNKSRALPDDPPHDMFGHGT
                     ACAGIIHSLAPEAELYSVRVLGKDLSGRAMQFAGGLRWAIENGMHVVNLSLSTSMQEY
                     YAIFHELTDDAYFHNVALVSAVNNIPVPSYPSLYSSVFSVAAHEEKDPFTFYYNPNPP
                     VEFGAPGIDIDVAWLDNDYTVGTGNSFAAPHIAGIITLIRSKHPELTPFQIKTVLYAC
                     ANNVQHHSAHQSAKS"
     gene            complement(2056..2838)
                     /locus_tag="VFF70_04305"
     CDS             complement(2056..2838)
                     /locus_tag="VFF70_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY43952.1"
                     /translation="MNDQSTHDGKLADQLRRLLEAIEASGHAILPSSNRELLQSIVDA
                     AARIFGAAAASIALLNEKDQMLEFKVSYGIGNENVVGMRLPMDKGIAGYVIMTGQPLA
                     ISNLQQDKRFNKDFAQTTGYVPKSILAMPMMYGERLIGVMEVLDKISSPSFGMKDMEL
                     LAMFAQQAAIAIHQSQQYDMIGQALVHGIKELAMTDPDAQSAEIFQVIEAATIEDQRG
                     RELLELAGIFNSIADLGEAERTACLKILTAFRDFAKSKSAYA"
     gene            complement(2942..>3275)
                     /locus_tag="VFF70_04310"
     CDS             complement(2942..>3275)
                     /locus_tag="VFF70_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43953.1"
                     /translation="AKTAAKNEVMGHSMTRSKSVGAKTAAKNEVMGHSMTRSKSVGAK
                     TAAKTEVMGHSMSRSKSVGAKTAAKTEVMGHSMTRAKSKAASRIKANNIDDIDFDPTK
                     GTYIVREG"
BASE COUNT          694 a          916 c          873 g          792 t
ORIGIN      
        1 tggaaaatct gatccaagtg ttgggcgatg aacattcaca attcgaatca ccgagcgcgg
       61 tagccgactc aaaagccgcg ttggccggca ccatcaatta cgttgggatt ggcgtcctga
      121 tcctgccact gccggataaa aaacgagcga cggtactggc gctgctgccc aattcttccg
      181 cggcgcatga tggacgtctg catccgcatg atgcgattct ggcagtggac ggtctgccgt
      241 tggttgagaa cggtgtatcc tatccccagc gagtccgcgg cccggaatgt tcagcggtgg
      301 tgttgaccgt tcaatcgccc ggtcagagtc cgcgcgatat cacgctgctc cgttatcgag
      361 tcagtggcgc ggagccgatc ggtgcgcgtt tggtatcgac ggccgatggc gcgcgtatcg
      421 gttatctttt cctgccgacc tttttcgatg aaaccattcc ggcccaggtc aaaaaagcat
      481 tggaggattt cggctcactg gatgggctga tcctggacaa tcgtatgaat ggcggtggat
      541 cgagcgacgt cctcaatcct gttttgagtt actttacttc aggaacgctc ggccgctttg
      601 tcagtcgaac ggtgacgcgc acactcagct tgaaagccga cccgatcaac acctcacaga
      661 cggtgccgct gatcgtcttg gtcggcgagg atacggtcag cttcggcgaa atctttgccg
      721 gaattttgca ggatgtcggc cgcgcaaaaa tcgtcggtca gaccacgctc ggcaacgtcg
      781 aaacgctgca cggctatgac tttcaagatg gatcgcggct gtggattgcg caggagcgat
      841 tcgatccgga gcaatcgcat gccaattggg aacagaccgg catcattccc gatatgccag
      901 ccttcgccga ttgggacacg ttcacttttg aaaacgatcc gtccattgcg gcagcgctga
      961 aattattggg gcatgaatag atggtcaaga aaatatttga cgccttaaac aaaaccgccg
     1021 ctcgattgag cggcggttca tttattacag ctgacctcat gatttcgcac tttgatgtgc
     1081 gctgtgatgc tgcacgttgt tcgcacaggc atacaacacc gtcttgatct gaaacggcgt
     1141 caactctgga tgcttcgatc ggatcagtgt gatgatcccg gcaatatgcg gtgcggcgaa
     1201 actattgccc gtgcccaccg tataatcgtt gtccagccac gccacatcga tgtcgatgcc
     1261 gggcgcgcca aactcgaccg gcggattggg attgtaatag aaagtaaacg gatctttctc
     1321 ttcatgcgcg gcgactgaaa acacagatga atacaatgaa gggtaactcg gcacaggaat
     1381 gttattcacc gctgacacca acgcaacatt gtggaaatag gcgtcgtccg tgagttcgtg
     1441 gaagatggcg taatattcct gcatcgatgt gctcaggctg agattgacca cgtgcatccc
     1501 gttctcgatc gcccatctta gcccacccgc aaattgcatc gctcggcctg atagatcttt
     1561 acccaacacg cgcacgctat atagctcggc ctctggcgcg agcgagtgaa tgatcccggc
     1621 gcaggccgtg ccgtgaccga acatatcgtg cggcggatcg tccggcagcg cacgcgattt
     1681 atttttcaag cggccatcat attcaatagc gattccaccc ttgaccgcat cacccaccgc
     1741 cggatgatcg tgatcgatgc cgctgtccac gatggcgacc ttcgcccctt taccggtgct
     1801 gccaccccag gcccactcgc gcgtgatctc gcccatcggg ccgagtgaag atattgcttt
     1861 caatacatcg ggtgaaaatt gagaagacca cgccgggcgc acttcttctt cagattgttc
     1921 ttcatcggcc atatttgatc gcctttaggt tatgagtggc tggcttaata atatttttac
     1981 cgcaaagtgc caatagcgcg tggagataaa ctgacatatc gatgcctgct cagcagactg
     2041 gaagcctaaa tatttttacg catacgccga ttttgatttg gcaaaatcgc ggaaggctgt
     2101 caatattttc aaacaggccg tgcgctccgc ttcaccaaga tcggcgatcg agttgaagat
     2161 accggccaac tccagtagct cacgtccgcg ctgatcttcg atcgtcgccg cctcgatcac
     2221 ctggaaaatt tcggcggatt gtgcgtccgg atcggtcatc gccaactctt tgatgccgtg
     2281 caccaatgcc tgaccgatca tgtcgtactg ctgcgattga tgaatggcga tcgccgcttg
     2341 ctgcgcgaac atcgccagta gctccatgtc tttcatgccg aaagacggcg aactgatctt
     2401 gtccaacact tccatcacgc cgatcagtcg ctcaccatac atcatcggca tcgccagaat
     2461 cgatttgggc acatagccgg tggtctgcgc aaaatcttta ttgaagcgct tgtcttgctg
     2521 caagtttgag atggcaagcg gctggccggt catgatcaca tagcccgcga tgcccttatc
     2581 catcggcagg cgcatgccaa ccacgttctc attgccgatg ccatacgaga ctttgaattc
     2641 cagcatctga tctttttcgt tcagcagcgc gatcgaggcc gccgccgcgc caaagatgcg
     2701 cgcggccgca tcgacgatcg actgcagcaa ttctcgattg gaactcggca gaatggcgtg
     2761 cccgctcgct tcgatcgcct ccagcaatcg ccgcagctga tccgctaatt tgccatcgtg
     2821 tgttgattga tcattcatcg cgttgctcct gtgtagtgga ctgcgctctg cgtgaataca
     2881 agcataccca gaaggaaatg cttctgggta tgcaaacggt tacttgaagt tcgttaaaag
     2941 gttagccttc gcgaacgatg taagtacctt tggtgggatc gaagtcgata tcatcaatgt
     3001 tattcgcctt gatccggctg gcagcttttg acttggctcg cgtcatggag tgacccatca
     3061 cttcggtctt ggccgcggtc ttggcaccaa ctgatttgct gcgcgacatc gagtgaccca
     3121 tcacttcggt cttggccgcg gtcttggcgc cgaccgactt gctgcgggtc atcgagtggc
     3181 ccatcacttc gttcttcgcg gccgtcttgg cgccgaccga tttgctgcgg gtcatcgagt
     3241 gacccatcac ttcgttcttg gcggccgtct tggcg
//
LOCUS       DASRTJ010000204         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_49940,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000204 DASRTJ010000000
VERSION     DASRTJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49940"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1367
                     /locus_tag="VFF70_04315"
     CDS             252..1367
                     /locus_tag="VFF70_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43954.1"
                     /translation="MRHRFVFLITVLLNMIWPSSAWASAPQPPDCREETPAYLATIAD
                     PANLLEYFHDWELQSVRACQGGSTEVFLQYVLPAAYSSLGLTYNVTVRFTLQDNAIAY
                     GDQLRPLYSDYRARIAAFENDPGVKRFVQITGSTDVDNADADAFQINPHGRDGLTFSS
                     APNAVTHFTFVNPHLIEDLAFFVPVVPGIPQIAEFRRHYPIGYSRFDDDRLYIAKDAE
                     CPDCNDRIVFDLDPLQVRVHSYSLSNNFTWETLPEIAQAHRFVEEHLLKEGLPNCEIG
                     HGDLHTYTMASYRYGSTDRSVTVALKCDGGIHWKDAGLILHPDGTYERLRVEFDYHAT
                     PTPRSPGSNLGCFLAPIALVAVSTCVWPTRRRRTHDK"
     gene            complement(1425..2405)
                     /locus_tag="VFF70_04320"
     CDS             complement(1425..2405)
                     /locus_tag="VFF70_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43955.1"
                     /translation="MSDTVIEVDKLTKRFGDFTAVNHVTFNVQAGEVLGYVGPNGSGK
                     TTTIRMLLGLLQPSDGNANVLGYNIRTEAESIRPLVGYMSQKFALYDELTMRENLDFY
                     AGVYDVPRRDRSNRVHEILELLSLTKRQNDRASDLSGGWRQRLALGTALVHQPKLLFL
                     DEPTSGVDPSARRTFWDLIYELASRGTSIFVTTHYMDEAEHCNRVGIMFRGQLLAMDT
                     PSGLKASALPGAAWDVSLDRGSAALIAALTELHDVPGIVRAGLASDRLRMITAPNAHT
                     LESLKIVMNQAGFAEAAIEQVEPTLEDVFIALAGHGADERSSTNGMRRSG"
     gene            complement(2429..>2960)
                     /locus_tag="VFF70_04325"
     CDS             complement(2429..>2960)
                     /locus_tag="VFF70_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY43956.1"
                     /translation="LSGGMKQKLGLATALVHRPRVLLLDEPTGGVDPVTRQDFWQLII
                     RIVAQEDVSVVISTPYMDEASRCTRVGFISRGKIITEGTPGQVTSHLKDRVLELAGEP
                     LAALRHTATTDPDVEDVQVFGDRLHLRLRPDSSQRVIDRLPIALQARGVRVQRIRSIP
                     PTLEDVFIELSEQVNN"
BASE COUNT          624 a          937 c          737 g          662 t
ORIGIN      
        1 gtttcttgca agcaatcttc ggcatccagc gtgtcgcgca acatacgcca cacatacgca
       61 aaaatctccg cgctgtggtg ctccaccaac acttcaaagt ccggtttcac catgaccccg
      121 cctgtcagcc atttctaccg aatataaccc cgcaccgtag aatttgtgaa atgcccaggt
      181 ttcccgttag cggaactctc ccgatcggcg gagcgtcttt tcaggtagaa tgtttatatt
      241 aggagcggat gatgcgccat cgatttgtgt ttctcatcac cgtgctgctc aacatgatct
      301 ggccgtcgag cgcgtgggct tccgcgccac agccgcccga ttgtcgcgaa gaaaccccgg
      361 cctacctggc gaccatcgcc gatcccgcca acctcctgga atactttcac gactgggagc
      421 tgcagtctgt gcgcgcctgt cagggcggca gcacggaagt attcctacaa tacgttctgc
      481 ccgccgccta ttcgtcgctg gggctgacgt acaacgtcac tgtcaggttc acgcttcaag
      541 acaacgccat cgcctacggc gatcaacttc gaccactcta cagcgactat cgcgcgcgca
      601 ttgccgcctt cgaaaacgat cctggcgtta aacgttttgt tcaaatcaca ggctccaccg
      661 acgtggataa cgcggatgcc gacgcgtttc aaatcaatcc acatggacgc gacggcctta
      721 cgttttccag cgcgcccaat gctgtgacgc actttacctt cgttaatccg cacctgatcg
      781 aagacttggc cttcttcgtc cccgtcgttc ccggcattcc gcagattgcc gaatttcgcc
      841 gccactaccc gatcggctac tcccggttcg acgacgaccg tctttacatc gccaaagacg
      901 ccgagtgccc ggattgcaac gatcgtatcg tcttcgacct ggatccgcta caggtgcgtg
      961 tccattcgta cagcctcagt aataacttca catgggagac tctgccggag attgcacagg
     1021 cgcatcgctt cgtggaggaa cacttgttga aagagggact ccccaactgc gagatcggtc
     1081 acggggactt gcatacctac acgatggcct cctatcgtta tggttcgacc gaccgctcgg
     1141 tcactgtcgc tctcaagtgt gatggcggaa ttcactggaa agatgctggc ttgatcctcc
     1201 atccggacgg tacttacgag cgcctgcgcg tcgagttcga ttatcacgcc acgcctactc
     1261 ctcgctcgcc tgggtcaaat ctcggctgct tcttggctcc gatcgcatta gtggctgtgt
     1321 cgacctgcgt gtggcctacc cggcgcaggc gtactcacga caaatgatcg gaaccaacta
     1381 cggccctgag cggccacgac cgcgccgcgt tgtagccctc cgcttcatcc gcttctccgc
     1441 atcccattcg tcgaagacct ctcatctgcc ccgtgtcccg ccaacgcgat gaagacatct
     1501 tccaatgtcg gttccacttg ctcgatggcc gcctcagcaa aacccgcctg gttcatgaca
     1561 atctttaatg attcaagtgt atgcgcattg ggcgcagtga tcatgcgtag tcgatcggag
     1621 gccaggcccg cgcgcacaat accgggtacg tcatgcagct cggtgagcgc cgcgatcaat
     1681 gccgcgctgc cgcgatcgag gctgacatcc caggccgcgc cgggcagcgc cgacgccttc
     1741 aagccgctcg gcgtatccat cgccagcagc tgtccgcgaa acatgatacc cactcgattg
     1801 caatgctcgg cctcgtccat gtaatgcgtc gtcacaaaaa tcgatgtgcc gcgcgaggcg
     1861 agttcgtaga tcaaatccca gaatgtgcgc cgcgccgatg gatcgactcc gctcgtcggc
     1921 tcatcgagaa acaagagttt cggttgatgc actaaagcgg tgcccagggc caggcgctgc
     1981 cgccagccgc cggacagatc gctcgcccga tcattctgtc gcttggtcag gctgagcagc
     2041 tccaatattt cgtgtacacg gttcgatcga tctcggcgcg gcacatcata cacgccggcg
     2101 taaaaatcca aattttcgcg catcgtcaac tcgtcataca acgcaaactt ctgcgacatg
     2161 taaccgacca gaggccggat cgattccgct tcggtgcgaa tgttgtaacc caatacattg
     2221 gcgttgccat ccgaaggttg cagcaaaccc aacaacatgc ggatcgtcgt cgtcttgccg
     2281 gaaccgttcg ggccaacata gcccagcact tcaccggctt gcacgttaaa ggtcacgtga
     2341 ttcaccgcgg tgaaatcgcc gaagcgcttt gtgagtttat cgacttcaat aaccgtatca
     2401 ctcattgttc attgttcatt gaaaattgtt aattgtttac ttgttccgat agttcgataa
     2461 aaacatcttc aagcgtcggc ggaatcgatc ggatccgctg cacccgcacg ccccgggctt
     2521 gcagcgcgat cggcaatcga tcgatcacgc gttggctgct gtccggccgc agacgcaaat
     2581 gcaatcgatc gccaaagact tgcacatctt ccacatccgg atcagttgta gccgtatggc
     2641 gcaacgcagc gagtggttca ccggctaatt ccaacacccg atccttcaag tgactcgtca
     2701 cttgtcccgg cgtgccttcc gtgatgattt tgccgcggct gataaaaccc acgcgtgtgc
     2761 agcgcgaagc ctcatccata tatggcgtgc tgatcacgac cgagacatct tcttgagcga
     2821 cgatgcgaat aattagctgc cagaagtcct gcctcgtcac cggatcgaca ccgcccgtcg
     2881 gttcatctaa taataaaact ctcggtcgat gcaccaacgc agtagctaag ccaagtttct
     2941 gcttcatgcc gcccgacagt
//
LOCUS       DASRTJ010000205         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_314881,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000205 DASRTJ010000000
VERSION     DASRTJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314881"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFF70_04330"
     CDS             <1..528
                     /locus_tag="VFF70_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZY43957.1"
                     /translation="VKDFDFEYRQIVIRDPKGGQDCFTILPETLIEPIKEHLARVKQL
                     HAEDLAKGYGAVWLPNAFVVKYPNANREWGWQFAFPSGKLADEVEAKTNRRWYTSETT
                     LPKTVRVAAQKSGLTKRIRPHTFRHCFATHLLESGYDIRTVQELLGHKDVKTTMIYTH
                     VLQRGGLAVRSPLDA"
     gene            563..1264
                     /locus_tag="VFF70_04335"
     CDS             563..1264
                     /locus_tag="VFF70_04335"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZY43958.1"
                     /translation="MRKRWGVLLAFIAVGLIAFIIGGPPLISFLTDQARVQAWITSYG
                     ALGPLVLIGMIVLQTVLSISPVSLLAAASAYVFGFWGGVLYSGIGMAIGSSLNMLLGR
                     KLGRPFVEKLIAPKAIETFDRFNQQRGPLFYFIVFVMPWVPDDLACYSIGLSRLPLRL
                     MSVLAAIGRLPSVIVQAWLVTNANTLAPGVIIGVVVGGIVLALGFYRYHRKLEAWVID
                     LAARSHRNASIDEEA"
     gene            complement(1310..>1616)
                     /locus_tag="VFF70_04340"
     CDS             complement(1310..>1616)
                     /locus_tag="VFF70_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HZY43959.1"
                     /translation="ICTDAIDRYHSVMSDMTDTYGQLDRAEALARLDSIFLGQKTDWI
                     HEGTTDMRKQWHNLKYYTWVEQQGKSVEELNEQMHQEWWIAHQQMVGEIDKRITATR"
BASE COUNT          326 a          408 c          457 g          425 t
ORIGIN      
        1 gtcaaagatt ttgattttga atatcggcag attgtcattc gtgatcccaa aggcgggcag
       61 gattgtttta cgatcttgcc tgaaacttta attgaaccga tcaaagaaca tctagcacgc
      121 gtcaaacagc tgcatgcgga agacctcgcc aaaggctacg gcgcggtttg gctgcccaac
      181 gcttttgtgg tgaaatatcc aaatgccaac cgtgaatggg gctggcaatt tgccttcccg
      241 tccggcaaac tggcggatga ggtggaagca aaaacaaatc gccgttggta taccagcgaa
      301 acgacgctgc caaaaaccgt tagagttgcc gctcaaaagt cgggtcttac caaacgcatt
      361 aggcctcata cctttcggca ttgcttcgct acacatttac tggagagcgg ttatgatatt
      421 cgtaccgttc aggaattgct gggacataaa gatgtcaaaa cgacgatgat ctacacgcac
      481 gtcctccagc gcggtggctt ggcagtgcgc agtccactag acgcctgatt ctggtatagt
      541 ctcgaccatt cgggagtgtt cgatgcgtaa acgttgggga gtgctgcttg ctttcattgc
      601 ggtcgggttg attgcgttca tcatcggcgg accgccgctg atcagttttc tgaccgatca
      661 agcacgtgtg caggcatgga tcacaagtta cggcgcatta ggtccgctcg tcttgattgg
      721 catgatcgtt ttgcagaccg tgctgtcgat ctcgccggtc agtttgttgg cggcggcgtc
      781 ggcctacgtg tttggctttt ggggcggcgt gctctattca ggcatcggca tggcgatcgg
      841 ttcgtcgctc aatatgctct tgggccgcaa gttgggccgg ccgttcgtgg agaagttgat
      901 cgctccgaaa gcgatcgaga cattcgatcg attcaatcag cagcgcggac cgttgttcta
      961 cttcatcgtc tttgtcatgc cgtgggtgcc cgatgatctt gcttgttact cgatcggttt
     1021 gtcgcgcttg ccgttgaggt tgatgagcgt gctggcggcc atcggccgat tgcccagcgt
     1081 catcgttcaa gcgtggttag tcacgaatgc gaatacgctg gcgccaggag tgatcatcgg
     1141 ggtcgtcgtt ggcgggatcg ttttagcgtt ggggttctat cgctatcacc gcaaactgga
     1201 agcgtgggtc atcgatctgg cggcgcggtc tcatcgcaac gcatcgatcg atgaagaagc
     1261 ataatcgata aaaaacttcc gaaggccggc gacttcggaa gtatttgttt cagcgggtgg
     1321 cggtgatccg cttatcgatc tcgccgacca tctgttgatg ggcgatccac cactcttgat
     1381 gcatctgctc gttcagttcc tcgactgact tgccctgctg ttcgacccat gtgtaatact
     1441 tcaagttgtg ccattgcttg cgcatatccg tcgtgccttc gtgaatccaa tcggttttct
     1501 gccccaggaa gatgctgtct aaacgcgcca gcgcttcagc gcgatccagc tggccgtatg
     1561 tatcggtcat gtcgctcatc accgaatgat agcgatcgat tgcatcggtg cagatg
//
LOCUS       DASRTJ010000206         6103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_270180,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000206 DASRTJ010000000
VERSION     DASRTJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270180"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFF70_04345"
     CDS             <1..332
                     /locus_tag="VFF70_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZY43960.1"
                     /translation="IDRPSRRLPWSLLNRRHEVLAIRLIDPREIELPDIGPIFMEDAE
                     TGDQLYVDTHDKKFRRRFAEAAQRRKLELNQSFKRAGVDALSLSTDEDLVRAIVRFAA
                     LRKQRRK"
     gene            409..1413
                     /locus_tag="VFF70_04350"
     CDS             409..1413
                     /locus_tag="VFF70_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY43961.1"
                     /translation="MSFISPEMFVLLIAIPLFVLLYLRLQHRRKRIAAAYNKFGLAQA
                     QGRDFGRRRHVPLIFFLIGLTILIVALARPQATVSLPKIQGTVILAFDVSGSMAADDL
                     KPTRMEAAKVAARDFVQRQPLTVQIGVVAFSDSGFNVQPPSNDQEAILAAIDRLSPTR
                     GTSLANGILISLKTLFAGGPQPDELYSALLLTPTPTPEPVPAGSYNSAAIVLLTDGEN
                     TVNPDPIEAAQASADRGVRVYTVGIGSAAGTTLHVDGFTVHTQLDEVMLKQIAEVTDG
                     EYYQAENEADLRAIYDQLGQQLVIKPEATEITALFAGAGIIILLIGGFLSLVWFSRWP
                     "
     gene            1495..2568
                     /locus_tag="VFF70_04355"
     CDS             1495..2568
                     /locus_tag="VFF70_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY43962.1"
                     /translation="MQLQWPGFLFLLILIPLLIALYILILRRRRRLVVRYSSLSLIRE
                     ALPKQSGLKRHLPFALFLIALASLIVAVARPISIISVASDQTTIALVIDVSGSMCQTD
                     IQPSRLQAAEAAALAFVQHQKNSTQIGLIAFSGFAEIIQAPTTDPEALQAAIESLVVG
                     RRTAIGSGILKAIDAIAEIDPAVAPSIAPDSTATGPTPVPQGAYVPDIIVLLTDGVSN
                     AGPDPLDAAQQALDRGVRVYTIGFGTETGAEFSPICRGAFPNGGNFGRQFGGGQFGGG
                     QPGGGFRRGIDDVTLKQVADMTGGSYYSASSADELQNVFQNLPTYLITKHEVAEISFI
                     FVAIGALLAALAIMLSLWWHPSP"
     gene            complement(2656..2889)
                     /locus_tag="VFF70_04360"
     CDS             complement(2656..2889)
                     /locus_tag="VFF70_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY43963.1"
                     /translation="MSNNDHVAAQGSIEDGLKRLSPDTHSIVIDGGNHGQFGDYSPQS
                     GDGTATINAAEQWQQTVTAVMQLAIALRNDAIN"
     gene            complement(2903..4783)
                     /locus_tag="VFF70_04365"
     CDS             complement(2903..4783)
                     /locus_tag="VFF70_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZY43964.1"
                     /translation="MLNSKIDVLVIGGASLDTLHLATGQTVSAAGGAGLYTALGAARS
                     GAQAGMFAPRPDPMPIELQPAADRLTWIGPSISPDQLPHFEIAHYGQGRAELVNATWG
                     GEMLLTPEMLPDDLLGARFVHVAALRNTDRQFNFAKACKAKGARVSAGTYGHICATEP
                     DRVRALFDLVDLFFMNENEATLLFGSVEAARTQPGHILFITLSARGALVLQGEHATHV
                     PGVPAIELDPTGAGDTFCGATLAALTRGDHPIMAAKSAALLAAELIGAVGPMRLLSDQ
                     PAPNIPLDDRCQPVPAQIIRVADLISRLPEVQPFNFAGDFFPSIHHPHALDFFFAVTL
                     QQFSFWEFTSHSLTPSPDNLAATSANQRLRSHQERGTRKYSRPMIATIDGRTLKGSDY
                     LFYAYLRQLDREPDFFSPSRQAALTLADLEYVFRADDGSNPMPAIDLHLELANSFGRD
                     LLSMNVTPDQLVRFANEAEHPRQTFLTLLDHIGGYKEDPLRKKSMLLALILEQRPEKF
                     LRPAPHEPEPPVIDYHLMRSCLRTGLIEITDEELRRRLIGREELSAEEEWAVRFAAYQ
                     VIQQVQIASNRPMGAVDQFFFGARRRCPEMTEPDCPACAIDPVCAHRKELFQPVLRTT
                     FY"
     gene            4991..5458
                     /locus_tag="VFF70_04370"
     CDS             4991..5458
                     /locus_tag="VFF70_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZY43965.1"
                     /translation="MYKRILVTLDSSPLAEQVLPHVKGLIDGRPEVAIYLLSIAQVID
                     AAAASAMLYPLAVYPGRPIDEQAERRRLEDELCNYLRSIELQLNQNGNAVNCEVRFGQ
                     PAEEIISYAHDIRADLIAMSTHGRSGLARWTYGSVADRVLHAAECPVLLVRTK"
     gene            5557..>6103
                     /locus_tag="VFF70_04375"
     CDS             5557..>6103
                     /locus_tag="VFF70_04375"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZY43966.1"
                     /translation="MDIGVPRERREGEYRVGLTPIGVQLLSAEGHAVYIEKNAGVGAG
                     FSDRDYEQAGGRIVYSTQEVYNRADLVLKVTRPMNDEIEWLRDGQTLIGFLHLAAAQE
                     SKVETLLRKHVTAIGYEMVQADDGSLPVLIPLSQAAGRMIPQVAATLLQNNHGGKGIL
                     LGGVPGVPPAEVVIVGAGTVGT"
BASE COUNT         1361 a         1576 c         1744 g         1422 t
ORIGIN      
        1 caattgatcg cccgtctcgg cgtcttccat ggagtttgct caatcggcgg catgaggtcc
       61 tggccatccg cttgatcgat ccgcgcgaga tcgaactgcc ggacatcggt ccgatcttca
      121 tggaagacgc cgagacgggc gatcaattgt atgtcgatac gcacgacaaa aaattccggc
      181 ggcgttttgc cgaagcggcc cagcgacgca aattagaact gaatcagtct ttcaaacgcg
      241 cgggtgttga tgctttgtcc ctctcgaccg atgaagattt ggtgcgtgcc atcgtccgtt
      301 ttgcggcgtt gaggaagcaa agaagaaaat gatgaatgat gaatgcggag tgcggaatga
      361 agaaaggatt ccgcaatcga cattccgtat tccgaaatag ggttttctat gtctttcatc
      421 tcgcctgaga tgttcgttct tctaatagcc attccgctgt tcgtgctgct gtatctgcgt
      481 ttgcagcacc gacgaaaacg gatcgccgcg gcgtacaata aattcgggtt ggcgcaggcg
      541 caaggccgcg acttcggccg ccggcggcac gtgccgttga tcttctttct gatcggcctg
      601 acgatcttga ttgtcgcttt agcgcggccg caagcgaccg tgagcctgcc gaagattcaa
      661 ggcacggtca tcctcgcgtt cgatgtgtcg ggcagcatgg ccgccgatga tttaaagccg
      721 acgcgcatgg aagcggctaa agttgccgcg cgtgattttg tccagcgtca gccgctgacg
      781 gtgcagatcg gtgtggtggc ctttagtgac agcggcttca acgttcaacc accatcgaac
      841 gatcaagaag cgatcctcgc cgcgatcgat cgcttgtcgc cgacacgcgg cacatcatta
      901 gccaacggca ttctgatttc gctcaagact ttatttgcgg gcggaccgca gccggatgaa
      961 ctttacagtg ctttattgtt gacaccgacg cccacgcctg aaccagtccc ggccggtagt
     1021 tacaactcgg cggcgattgt gttgttgacc gatggcgaga ataccgtgaa tcccgatccg
     1081 atcgaagcgg cacaggcttc ggccgatcga ggtgtgagag tgtatacggt tggcatcggc
     1141 agtgcggcgg gcacaacgct gcacgtcgat ggcttcaccg ttcatacaca acttgatgaa
     1201 gtgatgctca agcagatcgc ggaggtcacc gacggcgaat attatcaggc ggagaacgaa
     1261 gcagatttgc gcgcgatata tgatcagctc ggccaacaat tggtaatcaa accggaggcc
     1321 accgagatca cggctttgtt cgcgggcgca ggcattatta ttttattgat tggcggtttc
     1381 ttgtcgttgg tctggttcag ccgctggccg tgaagagccg actaaagtcg gcgctccgga
     1441 acgccgactt tagtcggcag ttttcaaagg cacgtggtgg agggaggtag atcgatgcaa
     1501 ttgcaatggc cgggtttctt attcctgctc attctcattc cgctgttgat cgcgctttat
     1561 attttgatcc tgcgccgacg ccgacgcttg gtggtacgct attcaagttt aagtttgatt
     1621 cgtgaagcgc tgcctaaaca atcggggtta aagcgacacc tgccgtttgc attgtttctc
     1681 atcgcgctcg ccagtttgat cgtggcggtg gcgcggccga tatcgatcat tagtgtggcc
     1741 agcgatcaaa cgacgatcgc gctggtgatc gatgtatcgg gcagcatgtg tcagaccgac
     1801 attcaaccca gtcgtctgca ggcggccgag gcagcggccc tcgcttttgt tcaacatcaa
     1861 aagaacagca cgcagattgg cctcattgca ttctccggtt ttgccgaaat cattcaagcc
     1921 ccgacgactg atcctgaagc cttgcaagcc gcaattgaaa gtttggtcgt tggtcgccgc
     1981 acagcgatcg gtagtggtat cctgaaagcg atcgatgcga ttgccgagat cgatccggcc
     2041 gtcgcgccaa gtatagcccc ggactcaaca gcaaccgggc caacgcccgt gccgcagggc
     2101 gcgtatgtgc cggacatcat tgtgttgctg actgatggcg taagcaacgc cgggcctgat
     2161 ccgctcgatg ctgcgcagca agcgctcgat cgcggtgtgc gcgtttacac gattggtttt
     2221 ggcacggaga ccggcgcaga attcagtccc atctgccgcg gcgcttttcc aaacggcgga
     2281 aacttcggcc gacaatttgg cggcggtcag tttggcggcg gacaaccggg cggaggtttt
     2341 cggcgcggca tcgatgatgt gaccttgaag caagtcgccg atatgacagg tggcagttac
     2401 tattcagcct cgagtgcgga cgaactgcaa aatgtttttc aaaatttgcc cacctatctc
     2461 atcaccaaac atgaagtcgc ggagatcagt tttatctttg tcgccattgg cgcgctgttg
     2521 gctgcgctgg cgattatgct ctcactgtgg tggcatccat cgccttgagt ttcttgaggt
     2581 tacgcgaatt caatatgaaa acactcgatc gaattttcga tcggtaattt ttgtttccca
     2641 acgaagatgg tttgatcaat ttatcgcatc attcctcaac gctattgcca actgcatgac
     2701 cgctgtcacc gtttgctgcc actgttcagc tgcgttgatc gtcgccgtgc cgtcgccgga
     2761 ctgcgggctg taatcaccga actgtccgtg attgccgcca tcgatgacga tggaatgtgt
     2821 gtcgggtgac aaacgtttta gtccgtcttc aatgctgcct tgggccgcaa cgtgatcatt
     2881 attcgacatt ccgcactccg cattaataaa acgttgtcct cagcaccggc tgaaacaatt
     2941 ccttgcgatg tgcgcacacc ggatcgatcg cacacgcggg gcaatccggc tcggtcattt
     3001 cgggacagcg ccgccgcgcg ccgaagaaga actgatcgac tgcgcccatc ggacgattcg
     3061 atgcgatctg cacttgctgg atcacttgat aggcggcaaa gcgcacggcc cattcttcct
     3121 ccgccgatag ttcctctcgc ccgattaatc ttcgccgcag ttcttcgtcg gtaatttcaa
     3181 tcaaaccggt gcgcagacaa gaccgcatca agtgataatc gatcacgggt ggttcgggtt
     3241 cgtgcggagc agggcgcaga aatttttcag gacgctgttc caggatcaac gccaacagca
     3301 tcgatttctt gcgcagcggg tcttctttgt agccaccgat gtgatccagc agagttaaaa
     3361 atgtttggcg cggatgctcg gcttcattag caaaacgcac caactgatcg ggtgtcacgt
     3421 tcatcgatag cagatctcgc ccgaaagagt ttgctagttc caaatgcaga tcgatcgcgg
     3481 gcatgggatt cgagccgtcg tcagcgcgaa aaacatattc tagatcagct aaagtgagcg
     3541 cagcctgtcg agatggcgaa aagaaatccg gttcacgatc gagttggcgc agataggcgt
     3601 aaaacaaata atcggaacct ttcagggtcc ggccatcgat ggtagcgatc atcggacgag
     3661 aatatttcct tgtccccctc tcctgatgac ttcttaatct ttgattggca gaagtcgctg
     3721 ccaagttatc aggggagggg gttagggagt gggacgtaaa ttcccagaaa ctaaattgct
     3781 gcaacgtcac tgcaaagaaa aaatccagcg catggggatg atggatcgat ggaaagaaat
     3841 caccagcgaa gttgaacggt tgaacttcgg gcaaacgcga tatgagatca gcaacgcgaa
     3901 tgatttgagc aggcacaggc tggcagcgat catcgagcgg aatgtttggg gcaggttgat
     3961 cagacagcaa gcgcatcggt ccaaccgcgc cgatcaattc tgcggccaac aaagccgcgg
     4021 atttggccgc catgattgga tgatcacctc gcgtcaacgc agcgagtgtt gccccgcaaa
     4081 atgtatcgcc ggcgccggtg ggatcaagct cgatcgcagg gacgccgggt acatgagtag
     4141 catgctcacc ctgcagcacc aatgcgccgc gagcgcttaa ggtaatgaat aggatatggc
     4201 cgggctgtgt gcgagcagct tcaacgctgc cgaataataa cgtcgcttca ttctcattca
     4261 tgaaaaataa atcgacgaga tcgaacagcg cgcgcactcg atcaggttcc gtggcgcaga
     4321 tgtgaccgta agtgccagct gaaacgcgcg cgccttttgc tttgcaggcc ttcgcaaaat
     4381 tgaattgtcg atcggtgtta cggagtgcgg ccacatgcac gaagcgcgca ccaagcagat
     4441 cgtctggcag catctccggc gtgagcagca tttcgccgcc ccatgttgcg ttgactaatt
     4501 cagcacgtcc ctgtccgtaa tgtgcgatct caaagtgtgg tagttgatcg ggtgagatgc
     4561 ttggtccgat ccacgttaat cgatctgcgg cgggctgcag ttcgatcggc atcggatcgg
     4621 gtcgcggcgc aaacatgcct gcttgcgcgc cggatcgagc cgctcctagg gcggtgtata
     4681 aaccggctcc gccagcggcc gaaacagttt gcccggtcgc gaggtgtaaa gtatccagcg
     4741 acgcgccgcc aataacgaga acatcaattt tggaattcag catactgcaa ttctaacatg
     4801 gtgtgattat tatcaacacc agatggcaaa cggctcttgg ctgaactctt tatctaacat
     4861 tcgcatgaac gtaggatggc agtatgacat tcagcctgaa gagatagcga cgttcctcac
     4921 tgcctgaatt ttagcgacaa tgttacccta aattaggttg ggcgtatcgg gattttcagg
     4981 aggcagagca atgtacaagc gcattctcgt cacgttggat agttcacctt tggcggagca
     5041 agtcctgccg catgtcaaag gtctgatcga cggccgcccc gaggttgcaa tctatctgct
     5101 gtcgatcgct caagtgatcg atgccgcggc cgcatcggcg atgctgtatc cgttggcggt
     5161 ttatcccggc cgcccgatcg atgaacaggc tgaacgccgt cggctcgaag atgagctgtg
     5221 caactatctg cgcagtattg aattgcagct caatcaaaat ggcaacgcgg ttaactgtga
     5281 agtgcgcttt ggccaaccgg ctgaagaaat catcagttac gcgcacgaca ttcgcgcgga
     5341 tttgatcgcc atgtcgacgc atggccgcag cggtctggcg cgctggacgt atggcagtgt
     5401 cgccgatcgg gtcttgcatg cggccgaatg tccggtattg ctggtgcgta cgaagtgaaa
     5461 gataatgaag tcagatcgat tgcagaatgt ggagatagag atttactttc tcgaccgctc
     5521 gatctgatat ctcgttttga ttgaggagat aaataaatgg acattggtgt tccgcgtgaa
     5581 cggcgggaag gtgaatatcg ggttggccta acgccgatcg gtgtgcagtt gctcagcgcc
     5641 gaagggcatg cagtctatat tgagaagaac gcaggcgtcg gggccggctt cagcgatcga
     5701 gattacgagc aggccggtgg ccggatcgtt tattcaacac aggaagttta caatcgcgcc
     5761 gatctcgtat tgaaagtgac gcggccgatg aacgatgaaa tcgaatggct gcgcgacggt
     5821 caaacattga ttggcttctt gcatttagcc gcagcccaag agtccaaagt ggagacactg
     5881 cttcgcaaac atgtgactgc catcggctat gagatggtcc aagccgatga tggatcgctg
     5941 ccggtcttga taccgctatc gcaagctgcc ggtcgcatga tccctcaagt cgcagctaca
     6001 ttactgcaaa ataatcatgg cggcaaaggg atattgttgg gcggcgttcc gggtgtgccg
     6061 ccggctgaag ttgtcattgt cggtgcgggc acggtgggca cgt
//
LOCUS       DASRTJ010000207         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_12207,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000207 DASRTJ010000000
VERSION     DASRTJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12207"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFF70_04380"
     CDS             <1..287
                     /locus_tag="VFF70_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HZY43967.1"
                     /translation="VDVVKPDEPLPNLPVARAVWIPQPDLKTAATAWILAGGAHHTGF
                     SQSVTTEHLEDFAAMADIEFLLINHDTRLSDFKKELRWNDLYYHLMPARR"
     gene            299..1390
                     /locus_tag="VFF70_04385"
     CDS             299..1390
                     /locus_tag="VFF70_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZY43968.1"
                     /translation="MTEFNEKQKRIRDVLATHHLDALVLRGVSSFAWATCGAASYVNT
                     ATTNGAASLLILPTGRYVITDNIEATRLKQEEQLEAQGWEFRIAPWYETNNAIAELTR
                     GLKLAADTAYPGAIDLSQDIARLRANLTPEEGERYRALSHLCAEAMDHAIRAVRPGLT
                     EYALAALLSGEVQRRGVSPIVNLIATDERIFNFRHPLPTDKKLDRYAMLIVCGRKWGL
                     VCSITRLVYFGHLPAELRRKAEVVARIDAAYISTTRPGQTLDEIFRRATDVYHETGFP
                     NEWQLHHQGGSAGYEPREFIATPTSTEKVTVGQAYAWNPSITGTKSEDTILVGPNGNE
                     IMTEIPGWPMLPIAIDGQTILRPAILEIT"
     gene            1421..3040
                     /locus_tag="VFF70_04390"
     CDS             1421..3040
                     /locus_tag="VFF70_04390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZY43969.1"
                     /translation="MAKHKYAIGVDTQNSIESGNTVLGIELGSTRIKAVLIGEDHLPI
                     ASGSYEWENRYENGIWTYRLEDVWTGLQASYRKLSQEAYEKYDTPLKTIGAIGFSAMM
                     HGYLAFDKGGNLLVPFRTWRNTITGQASEQLTELFQFNIPQRWSIAHLYQAILNKEAH
                     VKDISHLTTLAGYVHWKLTGQIVLGVGEASGMFPIDSQTNDYDEHMLALFNARLKAEN
                     ISWELQDILPKVLVAGDAAGVLTEEGAKLLDPAGRLQVGSALCPPEGDAGTGMVATNS
                     VAERTGNVSAGTSVFAMIVLEKALSKLYPEIDMVTTPTGKPVAMVHSNNCTSDLNAWV
                     DLFQEFTQALGVTRSESRLFEMLYQKALAGEADGGGLLAYNYFSGEPITHLEEGRPLF
                     VRTAESHFTLSNFMRVHLFSALGTLKIGLDILFEQERVKIDKILGHGGFFKTKEVGQK
                     IMAAAMNVPVSVMETAGEGGAWGIALLATYMLRKATDETLEGYLSNTVFAGETGATIA
                     PDQGDVNGFTVFMERYKKGLMIEKAAVAGLN"
     gene            3385..4461
                     /gene="chvE"
                     /locus_tag="VFF70_04395"
     CDS             3385..4461
                     /gene="chvE"
                     /locus_tag="VFF70_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HZY43970.1"
                     /translation="MVLALVAGCAAPVVAPTAAPVATAVPAAGAKIVGISMPTKTSTR
                     WISDGNSMVKAFEALGYKTDLQFADNDIQNQLSQIENMITKGANVLIIAAIDGSTMSD
                     VLQKAHDAGILVISYDRLITKTPNVDYYATFDNFGVGVIQGTQLVTGLGLKDGKTGPF
                     NVELFGGSPDDTNAGYFYNGAMSVIQPYIDSKQIVIQSGQMGMDKVGTLRWLAATAQA
                     RMDNLLSAFYTDKHVDGVLSPYDGLSIGILSSLKGVGYCTASLPCPVVTGQDAEVASV
                     KSIIAGEQRYTVFKDTRSLAAQTAKMVDQALKGQTVEINDTKTYNNGVKIVPSYLLIP
                     LSIDITNYLKELVDSGYIKAEDLK"
     gene            4630..>4797
                     /locus_tag="VFF70_04400"
     CDS             4630..>4797
                     /locus_tag="VFF70_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43971.1"
                     /translation="MSDVILEMRNITKTFPGVKALDNVSFSVVEGEIHALVGENGAGK
                     STLMKVLSGVYP"
BASE COUNT         1174 a         1243 c         1326 g         1054 t
ORIGIN      
        1 aggtggatgt cgtcaagccc gatgagccat tgcccaatct gccggtcgcg cgagcagtct
       61 ggattcccca acccgatcta aaaacagcgg cgaccgcttg gatactggca ggtggcgctc
      121 atcacacggg tttcagccaa tcggtgacaa ccgagcattt ggaagatttc gcggcgatgg
      181 cggacatcga gtttttgctg attaaccacg acacccggct ttcggatttc aagaaagagc
      241 tgcgctggaa cgatttgtat taccacttga tgccagcccg gcggtaatgg tggtcgacat
      301 gactgaattc aatgaaaagc aaaagcgcat tcgggatgtg ctcgccacgc atcatctcga
      361 tgccttggtt ctgcgtggtg ttagcagctt cgcctgggcg acatgcggcg cagcttcgta
      421 tgtgaataca gcaacgacga acggtgctgc ttcattgttg attctcccga cgggtcgcta
      481 tgtgattacg gataacattg aagcgacgcg cttgaagcag gaagagcagc tcgaagcgca
      541 aggctgggag tttcgcatcg cgccgtggta tgagacgaac aatgcgatcg cagagttgac
      601 acgcggcctg aaacttgcag ccgatacggc atatcccggc gcgatcgatc tatcgcagga
      661 catcgcgcgg ttgcgagcga acttgacgcc tgaagaaggc gagcgctatc gggcattgag
      721 ccacttgtgc gctgaggcaa tggatcatgc gattcgcgct gttcgacccg gcctgaccga
      781 atacgcgctg gcggccttgt tgagcggcga agtgcagcgg cgcggtgtta gcccgatcgt
      841 taatctcatc gcgactgatg aacgtatctt caacttccga catccattgc cgaccgacaa
      901 aaaactggat cgatatgcca tgctgatcgt gtgcgggcgg aagtggggcc tggtttgctc
      961 gatcacgcgg ctggtttact tcggtcactt gcccgctgag ctgcgtcgca aagctgaagt
     1021 ggtggcgcgc attgatgcgg cctacatctc aacaacgcgc cccggtcaaa ccttagacga
     1081 aatttttcga cgcgcgaccg atgtttatca tgagacaggt tttcctaatg agtggcagct
     1141 acaccatcaa ggtgggtcag ctggctatga accgcgcgaa ttcatcgcga cgccaacatc
     1201 gactgagaag gtgacagtag gtcaggccta tgcctggaat ccctcgatca ctggcacgaa
     1261 gtcggaagac acgatcctcg tcgggccgaa tggcaacgag atcatgaccg aaataccagg
     1321 ctggccaatg ctgccgatcg ctatcgatgg acagacgatc cttcggccag ccattcttga
     1381 aatcacttga atccgaaatc tggatcaata tgaggacaac atggctaaac acaaatatgc
     1441 tatcggggtt gacactcaaa attcaattga gagcggaaac acggtgcttg gcatcgagct
     1501 tggctcgact cgcatcaagg cggtgctaat cggcgaggac catctgccga tcgcctcggg
     1561 cagctatgaa tgggaaaacc gatatgaaaa cggcatctgg acataccgtc tcgaagatgt
     1621 ttggacaggc ttacaggcca gctatcggaa actgagccag gaggcttatg agaagtacga
     1681 cacgccgctc aagaccattg gcgccatcgg cttcagcgcc atgatgcacg gctatctggc
     1741 attcgacaag ggcgggaacc tactggtccc gtttcgcaca tggcgaaaca ctataaccgg
     1801 acaggcatcc gagcaactca ctgaactgtt ccaattcaat attcctcaac gttggagcat
     1861 tgcccatctt tatcaggcga tattgaacaa ggaagcccat gtcaaggata tcagtcatct
     1921 gaccacgctg gcaggctacg tccattggaa actgacgggg caaatagtgt tgggcgttgg
     1981 cgaagcatcg ggcatgttcc ccatcgacag ccagaccaac gattacgatg aacacatgct
     2041 cgcgttgttc aacgcacggc tcaaagcgga aaacatctcg tgggagctgc aagacatcct
     2101 gcctaaagtc cttgtagcag gagatgccgc aggcgttctg actgaagaag gcgcgaagtt
     2161 acttgatcct gcgggcagac tgcaagtggg tagcgcactc tgtccaccgg agggtgatgc
     2221 gggtaccggc atggtcgcga ccaacagcgt tgcagagcgg acgggcaatg tttccgcagg
     2281 cacatcggtc tttgccatga tcgtcttgga aaaggctctg tcgaagttgt atcccgagat
     2341 cgacatggtg acgacgccca ccggcaagcc tgtcgccatg gtccatagca acaactgcac
     2401 atccgacctg aatgcctggg ttgatctttt ccaggaattc acccaggcgc ttggtgtgac
     2461 gcggagtgaa tcccggctgt ttgaaatgtt gtatcaaaag gcgctcgccg gtgaggcgga
     2521 tggcggcgga cttctggcct ataattattt ttcgggcgaa cctatcacgc accttgaaga
     2581 gggacgcccg ttgttcgtcc gcacagccga aagccatttc acactttcaa atttcatgcg
     2641 ggtgcatctg ttctcagcgc tcggtacgct gaagatcgga ttggacatcc tctttgaaca
     2701 ggagcgggta aagatcgaca agatactcgg tcacggcggc ttcttcaaaa caaaggaagt
     2761 ggggcaaaag atcatggcgg cggcaatgaa tgttcccgtt tccgtgatgg agaccgcggg
     2821 agagggtggc gcatggggga ttgcgctgct ggcaacctat atgctccgta aggcaacgga
     2881 tgaaaccttg gaaggctatc tctccaacac ggtctttgca ggcgaaactg gcgcgacgat
     2941 agctcccgat cagggggatg tcaatggctt tactgtgttc atggaacgct acaaaaaggg
     3001 gcttatgatc gaaaaagccg ctgtggctgg gctgaactaa aagatttatg ctgccaaagt
     3061 gagaaagtaa acatgcttga gaaactcgag gaagaattgg tccaattaca tctaagccca
     3121 atcctgcggc gcagaaagcg tacgagaagt tgtatcgcga gtatgtgacg ctgcatgact
     3181 attttggagg aggtgccaat gacgtgatga aacgcttgaa acgtaccaaa gcagaaaccc
     3241 cggacgtata agaaatccaa aatcgtctag attggcgagc ctttcaaccg ggggagtgct
     3301 ctccaccaga caaaactcaa tgataaaaca aggaaaacaa aaatgaaaac ttctaagctg
     3361 ctctatcgga ttatgacgtt tgccatggtg ttagctctgg tcgctggttg cgctgcgcca
     3421 gtcgtcgcgc ctacggccgc gcccgttgct acagccgtac ctgcggctgg agccaagatc
     3481 gttggcattt cgatgccgac caagacttcg acccgctgga tttctgacgg caacagcatg
     3541 gtcaaagcct tcgaagcgtt gggctacaag accgacctgc agttcgccga caatgacatc
     3601 cagaaccagc tgtcccagat cgaaaacatg atcaccaagg gcgcgaacgt gctcatcatc
     3661 gccgccattg acggatcgac catgtccgat gtgctccaga aggctcacga tgccggtatc
     3721 ctcgtgattt cctacgaccg cctgatcacc aagaccccga atgtcgatta ctacgcgacc
     3781 ttcgacaact tcggcgtcgg cgttattcag ggtacccagt tggtcaccgg tctcggcctg
     3841 aaagatggca agactggccc gttcaatgtc gaactgttcg gcggctcgcc cgatgacacc
     3901 aacgccggct acttctacaa cggcgccatg tctgtgatcc agccttacat cgacagcaaa
     3961 caaatcgtca ttcaaagtgg ccaaatgggc atggacaagg ttggcaccct gcgctggctg
     4021 gccgcgacgg cccaggctcg catggacaat ctgctgagcg cgttttacac cgacaagcat
     4081 gtcgatggcg tcttgtcccc ctatgatggc ctcagcattg gtatcctctc ctccttgaag
     4141 ggcgttggtt attgcacggc cagcctgccc tgccccgtgg tgaccggcca ggatgcggaa
     4201 gttgcttccg tcaagtcgat catcgccggc gaacaacgct acactgtttt caaggatacc
     4261 cgctcgttgg ccgctcagac cgccaagatg gttgatcagg cgctgaaggg ccagaccgtg
     4321 gagatcaatg acaccaagac ctacaacaac ggtgtcaaga ttgtgccttc ctatctgctg
     4381 atcccgctta gcatcgatat caccaactac ctgaaggaat tggttgatag cggctatatc
     4441 aaagcagaag atttgaaata aggtgttacc gcaagagtgt gagggcattt gccctcacac
     4501 tcttgcctct ttttctggca ttattgttcg gtttcaatta gacttatctt tgaaagtcat
     4561 tatttttgaa ttataattga acgcattgca aggcaattcc actccatatc gatcaaggag
     4621 gagaaaacca tgtctgatgt aattctggaa atgcgtaaca tcacaaagac cttccctggc
     4681 gttaaggctc ttgataatgt gagctttagc gttgtggaag gtgagatcca tgcgttggta
     4741 ggcgagaatg gggcggggaa atcgaccttg atgaaagtgt taagcggtgt gtatcca
//
LOCUS       DASRTJ010000208         5531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_156668,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000208 DASRTJ010000000
VERSION     DASRTJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156668"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..3595)
                     /gene="mfd"
                     /locus_tag="VFF70_04405"
     CDS             complement(56..3595)
                     /gene="mfd"
                     /locus_tag="VFF70_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZY43972.1"
                     /translation="MTTSISLAGLLPLLREHQQFAQLREEIRSPHEGEWHLPLLRSAR
                     SFWLAAAAYETQRSILMVAARADRTQALHESIAAFLGNDSAHQLLRLPEPTTLFYERA
                     PWAREIVAERLNVLTQLSQPQASTSIVVASVRALMFRTLPPRAFKLGTRILKQGQAIN
                     LDQLLDAWIGFGYESTSTVLAPGEFSRRGGIVDIYPPALNQPIRIEFFGDEIESLRTF
                     DPASQRSLDPIEQITITPAIEALARNGSFAAEKIKEWDTANLSADLQQQFEKDRQALR
                     LNSSFRGIEFYLPLLHSDSTSVLDYFANDTLVVLDDADEIADAWSEFEEEALELRREA
                     TEGGTLPPNFPLPYITWDEWREKIDRRGVIALGQATADEAEHSLAASLPFSSNQRFGG
                     QLKTFIEHLAQIRSMNDGVIVISRQASRLAELWAEHAALIHPIGQVIDPPQPRSILFV
                     QGMLDDGFTLRGLTPIDADERRSIGWWSLHVITDAEIFGWARPQPRKRVRPTAITPEQ
                     FFADIQPGDYVVHVDHGVGLFQGLIKLTLEGVETEYLQVVYAGSDKLFVPIHQADRLN
                     KYIGAGDRAPEIHRLGTADWSQVRSKAKEAAIEVARELLELYAARELAPGYAFSIDTA
                     WQAELEASFPYIETEDQLRAIRAVKADMEKPRPMDRLICGDVGYGKTEVALRAAFKAV
                     QDGKQVAILVPTTILAQQHYTTFTSRLAAFPIGVEMLSRFRSDKEQSEILNRLRDGSI
                     DIIIGTHRLLQTDVVFKNMGLLIIDEEQRFGVTHKEQLKRMRTEVDIITLTATPIPRT
                     LYMSLVGVRDISTIDTPPEERLPVATYVGEYDDHLVRQAILRELDRGGQVFFVHNRVL
                     GIEQIEQRLKDVVPEARIGIGHGQMHEHELEKAMIAFVKGDIDVLLCTSIIESGLDIP
                     NANTLIIDRSDHFGLAQLYQLRGRVGRGAVRAYTYFLYDKISGLTEDARRRLEAIREA
                     SELGMGYSIAMRDLEIRGAGDLLGMRQSGQISAVGFDLYTKLLQRSVAELRAVRDNTA
                     PPPAQLTGVNLDLPLQAYIPTDYIGSESLRLQLYRRMGNIATPQEIDAMAQELADRFG
                     PVPKSVDNLMFQLHLKVLAAHALVSAIVRDREHNRLVVRSEALERVDRVGLQNRIRDL
                     GSVQRREIHVPLSSGNEWRTTVTRVLEEMNTNR"
     gene            complement(3696..4223)
                     /locus_tag="VFF70_04410"
     CDS             complement(3696..4223)
                     /locus_tag="VFF70_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43973.1"
                     /translation="MSASDLEQFAASFRCPSAMAEPTVNRIRQRVGTDVPMQEILAAI
                     KKIAPSRLSTDTLVARLQKDALIRLAKPVRSDSAKGSAPADRSGKPRSKVASAKSQIG
                     DLLAHNWSKAKADGVDTPDLSIDKFVRAAQSAAGEPKPTVARVLAAVEQLAKRDVIIT
                     PNLVVDEIVEADKIS"
     gene            complement(4229..4798)
                     /gene="pth"
                     /locus_tag="VFF70_04415"
     CDS             complement(4229..4798)
                     /gene="pth"
                     /locus_tag="VFF70_04415"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZY43974.1"
                     /translation="MNLIVGLGNPGREYARNRHNIGFRVADHFTYVHAINFSKVQLNA
                     MVAQGRLGETRVILAKPQTFMNVSGRAVGGLLNFYKVPIDRLLVVFDDLDLPFGSVRL
                     RQEGSAGGHNGMRSIISQLGGNTFPRLRIGIGRPPGRMDPAAYVLQDFNTDESIELEA
                     IVDRSVKAIETFIAEGIAMAMNQFNTTGE"
     gene            complement(4818..>5531)
                     /locus_tag="VFF70_04420"
     CDS             complement(4818..>5531)
                     /locus_tag="VFF70_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43975.1"
                     /translation="LITLLALPVQAAPLTQDAQATITSPADGQTVNGNVTISGSAIHA
                     DFDRFEISYGPDPNPNDTWQIFVNNNQMVAANTLGTWNTSVLVDGTYMIRLRVVRRDS
                     NYTEAFVRGIKVSNQQPIGTPTSVPPAPTFGPEATLPAIAPIIIEQPPTNAPIVSSST
                     TSNTAANNSQRAAGGNGSASLGSLMGSACISGLVWTFAVFCLFGAIIFGRSQFKRFLR
                     TQRKKLSDANTPSTPPSSA"
BASE COUNT         1176 a         1553 c         1562 g         1240 t
ORIGIN      
        1 ttcgaaagcg ttctgtgatt ccgtcatgat gattctccgt tatttgccgt ctcattcagc
       61 gattggtatt catctcttcc agaacacgcg ttacggttgt gcgccattcg ttgccagatg
      121 acagcggcac atgaatctca cgccgctgta ccgatcctaa gtcgcgaatc cgattttgta
      181 aaccaactcg atcgacgcgt tccagcgcct ccgatcgaac gacgaggcga ttgtgctccc
      241 gatcgcgcac gatcgcgctg actagcgcgt gtgcggccaa tactttcaaa tgaagttgga
      301 acatcaagtt gtcaaccgac ttgggcaccg gcccgaatcg atccgccagt tcttgtgcca
      361 ttgcatcgat ctcttgaggc gtggcgatgt tacccatccg ccgatacaat tgcaggcgca
      421 aactctcgct gccgatgtaa tcggttggaa tataagcttg cagcggcaga tcgaggttga
      481 cgccggtgag ttgcgcaggc ggcggcgcag tgttgtcgcg aaccgcgcgc agttcggcga
      541 ctgatcgttg caatagcttt gtatacaaat caaaaccgac cgcgctgatc tgaccgctct
      601 gccgcatacc cagcagatcg cccgcgccgc gaatctctag atcgcgcatc gcgatcgaat
      661 agcccatacc caactcgctc gcttcgcgga tcgcttcaag tctgcgccgt gcgtcttctg
      721 tcagcccgga aattttgtcg tataagaaat aagtgtaggc ccgcactgcg cctcgaccaa
      781 cgcggccgcg cagttgatac agctgcgcta aaccaaaatg atcgctgcga tcaatgatca
      841 aggtatttgc attcggaatg tcgagaccgc tctcgatgat cgacgtgcat aatagcacgt
      901 cgatgtcgcc tttcacaaaa gcgatcatcg ccttctcaag ttcgtgctcg tgcatctggc
      961 catgaccgat gccgatgcgc gcttccggca cgacgtcttt caggcgctgc tcgatctgct
     1021 cgatccccag cacacgattg tggacgaaga agacttgccc ccctcgatcg agttcgcgca
     1081 gaatcgcctg ccgtactaaa tgatcatcgt attcgcccac atacgttgcc acgggcaagc
     1141 gttcttccgg cggcgtatcg atcgtgctga tgtcgcgcac gccgaccaga ctcatataca
     1201 gggtgcgcgg gatcggcgtg gcggtcagcg tgatgatatc gacttcggtg cgcatgcgct
     1261 tcagttgctc tttgtgcgtt acaccgaagc gctgctcttc gtcgataatc aacaacccca
     1321 tgttcttgaa gaccacatcc gtttgtagca aacgatgtgt gccgatgatg atgtcgatcg
     1381 atccgtctcg cagccgattg aggatctcgc tctgttcttt gtccgatcgg aaacgcgaga
     1441 gcatctctac cccgatcgga aatgcggcca acctcgacgt gaaagtcgtg taatgctgtt
     1501 gcgccaggat ggtggttggg accaagatgg caacttgttt gccatcctgc accgctttga
     1561 aggcggcgcg cagcgctact tcagttttgc cgtaacccac atcgccgcag atcaaacgat
     1621 ccatcggccg tggcttttcc atatcagctt tgacggcccg aatagcgcgc agttggtctt
     1681 cggtctcgat gtatgggaac gaggcctcga gttcggcttg ccacgctgta tcgatcgaga
     1741 aggcatatcc cggcgcaagt tcgcgcgcgg catacaactc caacaactcg cgcgccactt
     1801 cgatcgcggc ctctttcgct ttcgatcgaa cttgcgacca atcggccgtg cccagtcgat
     1861 gtatctccgg cgcccgatcg ccagccccga tatatttgtt cagccgatcg gcctgatgaa
     1921 tcggcacgaa aagtttatcg ctgcccgcgt agacgacttg caaatattca gtctcaacgc
     1981 cctccagtgt caacttgatc aaaccttgaa acaaaccgac gccgtgatcg acgtgtacca
     2041 cgtaatcgcc gggctggatg tccgcaaaaa attgttcggg tgtaatcgca gtcggccgta
     2101 cacgcttgcg cggctgcgga cgcgcccaac cgaaaatttc tgcatcggtg atgacatgca
     2161 gtgaccacca gccgatcgat cgccgttcgt ctgcatcgat cggtgtgagg ccgcgcagcg
     2221 tgaaaccgtc gtcgagcatc ccctgcacaa agaggattga tcgaggctgc ggtggatcga
     2281 tcacttgccc gatcggatga atcagcgcag cgtgctcggc ccacagttca gccaggcggc
     2341 tggcctgccg gctgatcacg atcacgccgt cattcatcga tcggatttgg gctagatgct
     2401 caatgaaagt tttcagttgc ccgccaaatc tttgattgga cgaaaatggc agtgaagcgg
     2461 ctaaagaatg ttcggcctca tcggccgtcg cttgacccaa cgcgataaca ccgcgcctgt
     2521 cgatcttttc gcgccactcg tcccacgtga tatagggcag ggggaaattg ggcggcagcg
     2581 ttccgccctc ggttgcttcg cgccgcagct caagcgcctc ttcttcgaac tcgctccacg
     2641 cgtcggcaat ttcgtccgcg tcgtcgagca cgaccaatgt atcgttggca aagtaatcga
     2701 gcactgaagt tgaatcggaa tgtaacagcg gcagataaaa ttcgatgccg cgaaatgacg
     2761 agttgaggcg cagcgcttgt cgatcttttt caaattgctg ctgcagatcg gcgctgaggt
     2821 tggcggtgtc ccactctttg atcttctccg ctgcaaacga tccgttcctg gctaatgcct
     2881 cgatcgctgg cgtgatcgtg atctgctcga tcggatcaag cgatcgctgc gaggctggat
     2941 caaacgtccg cagcgattcg atctcatcgc cgaagaactc aatgcggatc ggctgattga
     3001 gcgcgggcgg ataaatatcg acgatgccgc cacgccggct gaactcgccg ggcgcgagaa
     3061 cagtcgatgt gctttcgtag ccgaaaccga tccacgcgtc caatagttga tcaaggttga
     3121 tcgcttgtcc ttgcttcaag attcgcgtgc caagtttgaa cgcgcgaggc ggcaaggtgc
     3181 ggaacatcag tgcgcggaca gatgcaacca cgatggatgt tgaagcttgg ggttgtgaga
     3241 gttgtgttaa aacattcagg cgctcggcca cgatctcgcg cgcccatggt gcgcgttcgt
     3301 aaaacagagt ggtgggttcg ggcaggcgca gaagttgatg cgcgctgtcg ttgcctaaga
     3361 aggcggcgat cgattcatgc agcgcctgcg tacgatcggc gcgggcggcc accatcaaga
     3421 tcgatcgctg agtctcataa gcggccgcgg ccagccaaaa tgagcgagcg gatcgcaata
     3481 gcggcagatg ccattcgcct tcatgcgggg atcgaatctc ttcacggagc tgtgcaaact
     3541 gctgatgctc gcgcagcaga ggcagcaaac cggcgaggct aatagaggtg gtcaatggta
     3601 attaacaatt ttcaatcccg cttcgcttgc gctgcgggga ctgcgtgaac aataatcaat
     3661 gccgaatgac caatgcccga tgccaaaagg agatgtcacg atatcttgtc ggcttcgaca
     3721 atttcatcga cgacaagatt cggtgtgatg atcacatcgc gtttggcgag ctgctcaaca
     3781 gctgccaaca ctcgggcgac tgttggcttt ggttcgcccg cggcggattg cgctgcgcgc
     3841 acaaatttat cgatcgagag atcgggcgtg tcgacgccgt cggctttggc cttggaccaa
     3901 ttgtgcgcca ggagatcgcc gatctggctc ttggcgctgg cgactttcga tcggggcttg
     3961 ccggatcgat cggctggagc ggaaccttta gctgaatccg atcggactgg cttggctaat
     4021 cggatcagcg catctttttg caagcgcgcc acgagcgtat cggttgacaa gcggctgggc
     4081 gcaatcttct taatcgcggc cagaatttcc tgcatcggca catcagtgcc aacccgctgc
     4141 cgaattcgat tgacggtcgg ctcggccatg gcactgggac agcggaagga agcggcaaat
     4201 tgctctaagt cactggcact catgatgatt actctcctgt tgtattgaac tgattcatcg
     4261 ccatcgcgat tccctcggcg atgaaagttt cgatggcttt taccgatcga tcgacaatgg
     4321 cttcaagctc gatcgattcg tctgtgttga agtcttgcag aacgtatgcg gccggatcca
     4381 tgcgtcccgg cgggcggccg atgccgatgc gcagccgcgg aaaagtgttg ccgcccaact
     4441 gcgaaatgat cgagcgcatc ccgttgtgac cgcctgcgct gccttcctgc cgcaaccgca
     4501 ccgaaccgaa aggcagatca agatcgtcga agactaccag caatcgatcg atcggcactt
     4561 tgtaaaaatt cagcaaaccg ccaacggcgc ggccgctgac attcataaag gtctgcggct
     4621 tggccagaat cacgcgggtc tcgcccagtc gaccctgcgc taccatggcg ttcagctgca
     4681 ctttgctgaa attgatcgcg tgaacgtagg taaaatgatc ggccacgcga aaaccgatgt
     4741 tgtgccgatt gcgggcgtac tcccggcccg gattgcctag cccgacgatc agattgatgt
     4801 tgccgccaga ctcatcacta ggcggatgat ggcggagtcg atggagtatt tgcatcggaa
     4861 agttttttgc gctgcgtgcg caggaatcgt ttaaactgac ttcgcccgaa aataatcgcg
     4921 ccgaataaac aaaaaacggc gaaggtccag acgagaccgc tgatgcaagc tgaacccatt
     4981 aaactgccta atgatgcact gccgttgcca cctgctgcgc gctgagaatt gttagccgca
     5041 gtgttgcttg tggtgctgga agaaacgatc ggcgcatttg tcggcggctg ctcgataatg
     5101 atcggagcga tggccgggag cgtcgcctca gggccaaagg tcggcgcagg cgggacggat
     5161 gtgggcgtgc cgatcggttg ttggttactc accttaatgc cgcgcacaaa tgcttcagtg
     5221 tagttgctgt cgcgccgaac gacgcgcagt cgaatcatat aggtcccatc aacgagcacg
     5281 gaggtattcc acgtgcctaa ggtgttggct gcaaccatct gattgttgtt gacgaaaatc
     5341 tgccacgtat cgttcgggtt cgggtcaggc ccataggaga tctcgaaccg gtcaaagtcg
     5401 gcatgaatgg ccgaaccgct gatcgtgaca ttgccgttga ctgtttgacc atcagccggc
     5461 gaggtgatgg tcgcctgtgc gtcttgagtc aacggcgcag cctggactgg cagcgctaat
     5521 aaagtaatga g
//
LOCUS       DASRTJ010000209         7160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_270297,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000209 DASRTJ010000000
VERSION     DASRTJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270297"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFF70_04425"
     CDS             complement(<1..319)
                     /locus_tag="VFF70_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY43976.1"
                     /translation="MFANLQKLRGPWLLIAILYGVELLDELIYGLHDAALPYFKTDFN
                     LTYTQIGLLFTLPGIVGFWLEPLIGLSGDTRHRRWLVIGGIVATAIGLAMIAVGQTYG
                     VILI"
     gene            complement(611..1396)
                     /locus_tag="VFF70_04430"
     CDS             complement(611..1396)
                     /locus_tag="VFF70_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43977.1"
                     /translation="MAKSRSRLLHSTRSAEPPTSDETAVANDRSTLFNQLALLFVVLT
                     PLALIVYLVIFLFPQLPINPLPPIKAGQVAIAGPTRTPVETSRPTRTPHPTNTPTPTA
                     TPAATDTPTVIVIVTVKPGSTRTRVPTATPIGPRTAVPTRSVFNYTAELTYQHHQLYG
                     SSWAGMAGLVFDANLKHQQNIDVHAWGDAPLGVNGQEALSGTYPQYGPSGWEFQVADK
                     PVFGTWHVQLIDDQGTPLSNVVDVVMQGDSRANLAYIIFQQNH"
     gene            complement(1398..2105)
                     /locus_tag="VFF70_04435"
     CDS             complement(1398..2105)
                     /locus_tag="VFF70_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43978.1"
                     /translation="MKRDQNYNQLALISLGLTLMAALYYLLIFVFPQLPINPLPPVSL
                     AALPADAVASSATPTPIDQISRPPTWTPTPPATPTSPRATWTPTSQSPTRTRVPTPTA
                     TLNPLIPTRSSFKFTANTPFFTSDPYGAACGNWGGVGGQVFNIDGSPLNGVSVVGWGG
                     PVAEQEKKVSASGIDSHLNHVYGNGAYELYIGAPGDFDFFVTLYENGQPVAPIIKLRM
                     TNDCAHDLVLINFQRNH"
     gene            complement(2165..2869)
                     /locus_tag="VFF70_04440"
     CDS             complement(2165..2869)
                     /locus_tag="VFF70_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43979.1"
                     /translation="MRRDRVYDLVATLCLMLAGIVAVYYLIVFVIPQSQLNPWSPIEM
                     PIAVNSSEVLASSTPEASPLPTSTATVTLTPTSTSTPIKVSSTMTFTPAPTVIFTRTL
                     APTTRPRSTITSTPDICSTLKLQAPKSGQHYNLNDSAQLSWTFARVLAVNEHFDVLFD
                     PPGQGIGSLAWADEDQPGLKNCTTRCTYSVGLGRYPGGRFNWTIGVIVGGRDHQVSAQ
                     VCKPPDPFNFTYLTLQ"
     gene            complement(2875..3594)
                     /locus_tag="VFF70_04445"
     CDS             complement(2875..3594)
                     /locus_tag="VFF70_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43980.1"
                     /translation="MADDSKVIPVGSKRGSRGLNTLSLIFIGLSILAGLYYAFVFILP
                     DTPLNPFRPIQIAQVVIPTLGSPTATVPPTWTPTPIGPSPTPRATITSAPTGTPRPTR
                     TPLPTETPTPSITPTPSPDLCSTLKLLGPPPGQRYNQYDNADLTWTFGRPLTAFEHFD
                     ILVGPPDRHGEPTSLSSAAWGDETDPANKNCTSYCTFTLALGGYNGGRTLWSVAVLRA
                     DKDRKVLGTICKAPPPYFFSR"
     gene            complement(3607..4464)
                     /locus_tag="VFF70_04450"
     CDS             complement(3607..4464)
                     /locus_tag="VFF70_04450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZY43981.1"
                     /translation="MPTTTLPRPASSIVPAASTAAPPNSADVAARVNGQDISMADFQK
                     EVANEQTSQNVDVNSANGQTQLVQIRQQVLDTLIDNMLIAQAAAKEGVIIIDADVDAA
                     IQKMITDSGGDAAFQQNLAAVGQTLTEARLSQKAGMLTNAMRDRVIAAISNTAEQVHA
                     RHILVDSEATAQALLAQLQAGVDFAQLAQQSSQDNLTRDRGGDLDWFPRGWLPAKEVE
                     DAAFALQPGQISGVVHSAFGYHILQVIERDPARTLSDQQVLRLQQQAMDNWLTGLRTA
                     AHVEIFVSQ"
     gene            4978..>7160
                     /locus_tag="VFF70_04455"
     CDS             4978..>7160
                     /locus_tag="VFF70_04455"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZY43982.1"
                     /translation="MTTQRTGWHNHPLIFGALAVLVIVGLLLPPISLLERLGITCAGT
                     SLDANTPATTTPDGLTVAVTDTAQPITLSVQSIDQAKFVAGESGDDYLAARDALPVRL
                     QLRSPIYQIKSCNAGHDASSLAINLPHAAQTANDTFDLYGWDGKTWSWLGAFLDTNSQ
                     TVSALVPTLPKNVALFQTASAAPYIGAQLKPGQSVPTDSNGLLNEVYVPGWSITADGS
                     LSAEAGTLPQAGNARIFPIVRDVAADGSINADVAHIVLSTEEAMQTHIAALTELAGRS
                     TFAGIAIDYRGLGDDDRANFAQFIQKLAAALQSQNKLLAVILPAPSIDSNGAPITGAY
                     DWPAIGAAADIVESDFGQDPTGYLNDGVSYALLNWATSQVSRNKFEAIVSIASLDKFN
                     NQTREVSFGDAIDPLGKLALTQPITVAGGSPVTLVLDNPDQVSDYKFDEATQTYRFNY
                     KRADVTHAIVINTAASLAQRLNGMLPRHARGVVLTGFTDDGIAADLAKVLTGYRQQSV
                     DSELPSGIQVTWSITSSTAGAVFTSTKPITDSTITWNAANEPGIYNVLASVQSISKGT
                     TVINITPAVSVTTGELTPVVAAPPCYSSAFVADVTIPDGTQLKNGETFTKTWRLTNNG
                     ECAWTDDTVLVFNSGTQMKAPASVQVGKVLTGTTADITITLTAPDQYGKYVGIWQLKN
                     AQGNFGTSMSAIIVAGEPPAGTVVASAPAAPIGNIGNFEIGGQING"
BASE COUNT         1704 a         1871 c         2013 g         1572 t
ORIGIN      
        1 caattaagat caccccgtaa gtttggccga ccgcgatcat cgccaaacca atcgcggtcg
       61 cgacgatacc accgatcacc agccaacgcc gatgacgtgt atcgccgctc aaaccaatca
      121 gcggttcaag ccagaagccg acgatgcccg gcagtgtgaa caacaagcca atttgggtat
      181 acgtgagatt gaaatcagtt ttgaagtaag gcaaagccgc atcatgcaat ccgtagatca
      241 gctcgtcaag cagctcgacg ccatacagaa ttgcaatcag caaccatggc ccgcgcagtt
      301 tttgcagatt agcaaacatg aatcgctcct tgatttctag ttagtgaatt ctcgatacgg
      361 aactaggaat caaggcggag cgcgagcgca gatcggggct aagaagttag ttgtgagtta
      421 acggggtgga tgaaagaaca ttttcgatct ctgatttctg attttcgttt agggttcaag
      481 ccatgcacgg tgaatccctt tacgcactat cacacccgcg caaacggacg cccagttttt
      541 cacactggag tccagcagct gataccgcgt cgggtaggta agacaatcag gatgataact
      601 ataagatcag ttagtgattc tgctgaaaga tgatatacgc caaattcgcg cgtgaatcac
      661 cctgcatgac aacatcaaca acattcgaca gtggagtgcc ctggtcatcg atcagctgga
      721 catgccatgt gccaaacaca ggtttatcgg ctacctggaa ttcccaacca gatggaccgt
      781 actgcggata ggttccagac aaagcttctt gtccattgac gcctaacggc gcatcgcccc
      841 aggcatgcac atcgatgttc tgttgatgct tgagattggc atcaaaaact aacccagcca
      901 ttccagccca actcgagccg taaagctgat gatgttgata ggtgagctcg gccgtatagt
      961 tgaacactga tcgcgtcggg acggcagtgc gtggcccgat cggtgttgcg gtcggcacac
     1021 gcgtgcgagt gctacccggt ttaacggtta cgattacaat aaccgtaggt gtatccgtcg
     1081 ccgcgggtgt cgcagtcggc gtcggcgtgt tggtgggatg cggggtgcgc gtcggccttg
     1141 atgtttcaac cggcgtgcgc gtcggtccgg cgattgcaac ttgccccgcc ttgatcggcg
     1201 gcagcggatt gatcggcagt tgtgggaaga ggaagatgac caagtagacg atcaaggcca
     1261 gcggcgtcag gacgacaaag agtaaggcca gttgattaaa cagggtcgat cgatcgttag
     1321 cgaccgccgt ttcatcgctg gtcggcggct cagccgatcg ggttgagtgt aacaatcggg
     1381 aacgggattt cgccatatta gtgattccgt tggaaattga tcaaaacgag atcgtgggcg
     1441 caatcgttcg tcatacgcaa cttgataatc ggcgctaccg gctggccgtt ttcatacagc
     1501 gtcacaaaga aatcgaaatc gccgggcgca ccgatataga gctcgtatgc accattgcca
     1561 tacacatggt tcaggtgcga atctattcca gacgcagaaa cttttttctc ttgttcggca
     1621 actggcccac cccatcctac cacactcact ccgtttaggg gtgacccatc aatattaaac
     1681 acttgcccac caactccgcc ccaattgcca caggccgcgc cataaggatc agaagtgaag
     1741 aaaggtgtgt tggccgtaaa cttgaatgac gatcgtgtcg ggatcaacgg attaagtgtg
     1801 gcagtgggtg tcggcactcg agtgcgcgtc ggcgactgag acgttggcgt ccaagtcgca
     1861 cgcggtgacg tgggggttgc aggtggcgtc ggcgtccaag tgggcggtcg cgaaatttga
     1921 tcgataggtg tcggtgttgc acttgaggcc acagcatcag caggtaaagc tgcaagtgag
     1981 acgggcggca gcggattgat cggcagctgc gggaagacga agatcagcag ataatataat
     2041 gccgccatga gagttaggcc taaactgata agcgccagtt gattgtaatt ttgatcgcgc
     2101 ttcatgaata ctcttttggc taaatgggca accgagttca atgcgctgga ctgcgacaac
     2161 tgcatcattg taaagtaaga taagtaaaat tgaaaggatc aggcggcttg cagacttgcg
     2221 ctgagacttg atgatcgcga ccgcctacga tgacaccgat cgtccaattg aaccgaccgc
     2281 ctggataccg gcccagtccg acgctatacg tacagcgcgt ggtgcagttc ttcaagccag
     2341 gttgatcttc atcggcccag gctaacgagc caatgccctg accgggtgga tcgaacaaga
     2401 catcaaagtg ttcgtttacc gccaagaccc gagcgaaggt ccaactcaac tgcgctgaat
     2461 cattcaaatt atagtgctgc cccgatttgg gtgcttgcag tttaagcgtg ctgcaaatat
     2521 caggcgttga tgtgatcgtc gaccttggcc tggtggttgg cgcgagcgtc cgagtgaaga
     2581 tcaccgtcgg cgctggagtg aaagtcattg ttgagctgac cttaatcggt gttgaagttg
     2641 aggtcggcgt taaagtcact gtggccgtcg acgttggtaa tgggctggct tcaggcgtgc
     2701 tgctggctaa gacctcgctg gaattcaccg cgatcggcat ctcgatcgga ctccagggat
     2761 tgagctgact ttgcgggatt acaaaaacga ttaggtagta gaccgcgacg atgcccgcca
     2821 gcatcaagca cagtgttgcc accaggtcat atacccgatc acgtctcatc aaattcaacg
     2881 gctgaagaaa tacggcggag gcgctttgca aatggtgccc agcaccttgc gatctttatc
     2941 cgcgcgcaaa acagccaccg accagagagt gcggccaccg ttatagccac ctaaggccag
     3001 cgtaaaagta caataggatg tacagttctt gttggcggga tcggtttcat cgccccaggc
     3061 cgcagatgac aggctggttg gttcaccatg ccgatcgggc ggcccgacta agatatcaaa
     3121 gtgttcgaag gccgtcaaag gccggccgaa cgtccaagtc aaatcggcat tgtcatattg
     3181 attataacgt tgaccgggcg gcggacctaa caacttcaac gtcgagcaga gatccggtga
     3241 tggagtcggc gtgatcgatg gcgttggcgt ttcagtcggc aacggcgtgc gcgttggacg
     3301 cggtgtgccc gtaggcgcag aggtaatcgt cgctcgaggt gtaggggacg gtccaattgg
     3361 cgtcggcgtc caagtcggcg ggacagttgc tgtcggcgag cccagcgtcg gtataaccac
     3421 ttgagcgatc tgaatcggcc gaaacggatt gagcggtgta tccggtaaga taaagacgaa
     3481 cgcataatac aaaccggcga ggatgctcaa gccgatgaag atcaagctca aggtattaag
     3541 cccgcgtgat ccgcgtttag acccgactgg aataactttg ctgtcgtccg ccatcattaa
     3601 ctccacttat tgactgacaa atatttcgac gtgggctgcg gtgcgcagtc cggtcagcca
     3661 attatccatc gcttgttgct gcaacctgag cacctgctga tcgctgagcg tgcgggcagg
     3721 atcacgctcg atcacttgga gaatgtgata accaaaagcg ctgtgtacca ctccgctgat
     3781 ttgaccaggc tgcagggcaa aggcggcatc ttccacttct ttggcaggca gccagccgcg
     3841 cgggaaccaa tcgagatcgc cgccgcgatc gcgggtgaga ttatcttgcg aagattgttg
     3901 agccaactgc gcgaaatcga caccggcttg aagttgcgcc agcagcgcct gtgcggttgc
     3961 ttcagaatca accaagatgt gtcgggcgtg aacttgctcg gcggtattgg agatggccgc
     4021 aatgactcga tcgcgcatgg cgttggtcaa catgccggct ttctgcgaga gacgcgcttc
     4081 agtcagcgtt tgacccaccg ccgcaagatt ttgctggaag gccgcatcac cgccggaatc
     4141 ggtaatcatt ttttgtatcg ccgcatcgac atcggcatca atgatgatga cgccttcttt
     4201 agccgctgcc tgcgcgatga gcatattatc gatcagcgtg tccaagactt gttggcggat
     4261 ctgaaccagc tgggtttggc cgttcgctga attgacatcg acgttttggg acgtctgctc
     4321 attcgcaact tctttttgga agtcggccat tgaaatatcc tgaccattca ctcgcgcggc
     4381 aacatccgcg ctgttgggcg gggcggccgt cgaggcagcc ggcacgatcg aggaagccgg
     4441 gcgcggcaaa gtagtcgttg gcactgtgcg gggtccagtt ggcgccggac tcgatccgca
     4501 cgcggaaaca atcaacgtga agaaaattaa acaagccaaa cgaagtgtat gggaaggtga
     4561 gacggatcga gtcaacaaag ttcctccacg ttgatcgaac tcgagaatgc ggctattata
     4621 ctaagcccat actaataatg caactaatcg aaagtaatgt ggctgacatt acgtcgatgc
     4681 aggggatgcg cttgcggatt caattgagcc atgttagaat cgcgcccacg atggtctgag
     4741 actatcgctc ggacattgag atactgtgac ggcgaggttt agccgtcact tttcatgaac
     4801 gctcctgaaa atgatcaaac ctgcgaagag cctgccctgc gcctagcggc ccactaactc
     4861 gcacgcgagt cgggtaaggg caacgaagtg aagggttgga attgagatta gtagatgaat
     4921 acttaaccct tcgccaggta cacacgaatt gttttcaagc gaaaggacat tacagatatg
     4981 acgacacaac gcacaggctg gcataaccat ccgcttattt ttggcgcgct ggctgtactg
     5041 gtgattgtgg gattgttgct gccgcccatc tccttattag aacgattggg gatcacctgt
     5101 gccggaacaa gtttggatgc gaacacaccc gcgacgacga cacccgatgg gttaaccgtc
     5161 gccgtaacgg atacagctca acctataacg ctgagcgttc aatcgatcga tcaagccaag
     5221 tttgtggcgg gcgaaagcgg cgatgattat ctggccgcgc gcgatgcgct gcccgtcagg
     5281 ttgcaattgc gcagcccgat ttatcaaatt aaatcttgca atgcagggca tgatgcatcc
     5341 agcctcgcca tcaacttacc ccatgctgcg cagactgcca acgatacatt cgatctatac
     5401 ggttgggatg gaaagacctg gtcgtggctg ggtgcgttct tggataccaa cagccaaacc
     5461 gtttccgctt tagtgccaac actgcccaag aatgtagctt tgttccaaac tgcttcagcc
     5521 gcgccttaca ttggcgcgca attgaagccg gggcagagtg tgccaaccga ctcgaacgga
     5581 ttgttaaatg aagtatatgt gcctggctgg tcgattacgg ccgatggatc actgtccgcc
     5641 gaagccggca cgttgccgca agccggcaac gcgcgcatct tcccgatcgt gagggacgtc
     5701 gccgccgatg gatccatcaa tgcggatgtc gcgcatattg tcttaagcac tgaagaagcg
     5761 atgcaaacgc atattgccgc tttgaccgaa ctggctggac gcagcacttt tgccggaatc
     5821 gccatcgatt atcgcggcct cggcgatgat gatcgagcga acttcgcgca gttcattcag
     5881 aaactcgccg ctgctctgca aagtcaaaac aagctactgg cggtgatcct gcccgcaccg
     5941 tcgatcgatt cgaatggcgc gccgattact ggcgcgtatg attggcccgc gatcggtgcc
     6001 gcggccgata tcgtcgagtc ggattttggg caagacccga ccggttatct caacgacggc
     6061 gtctcttacg cactgctcaa ttgggcaaca tcgcaagtga gccgcaataa atttgaagcc
     6121 attgtctcga tcgccagtct ggacaagttc aataatcaga cccgcgaagt ttcgttcggc
     6181 gatgcgatcg acccgctcgg taaactggcg ttgacccagc cgattacggt ggcgggcgga
     6241 tcgccggtaa cgttagtgtt ggataatcct gatcaagtca gcgattacaa attcgatgaa
     6301 gccactcaaa cctatcggtt caattacaag cgcgcggatg ttacccacgc gatcgtgatc
     6361 aatactgcgg cgagtctggc gcagcgcttg aacgggatgt tgccacgcca cgcgcgcggc
     6421 gtggtattaa ccggctttac ggatgatggc attgccgccg atctggcgaa agtgctgacg
     6481 ggttaccgac aacaatcggt ggacagtgaa ttgcccagcg gcattcaagt cacttggtcg
     6541 atcacatcct caacggccgg cgcagtcttc acttcaacta aacctatcac cgatagtacg
     6601 atcacttgga atgcagctaa cgaaccgggc atctataacg tgctcgcttc ggtacaatcg
     6661 atcagcaaag gcacgaccgt gataaatatt acaccggctg tgtccgtcac taccggcgag
     6721 ttgacccccg tcgtggccgc gccaccgtgc tacagttcgg ccttcgtcgc cgacgtgacc
     6781 attcccgacg gtacgcagtt gaagaatggc gagaccttca ccaagacatg gcggttgacg
     6841 aacaacggtg aatgcgcttg gactgatgac actgtcttag tgttcaattc gggcacgcag
     6901 atgaaagcgc ccgctagtgt gcaagttggt aaagtgctca ctggcacgac tgccgatatc
     6961 accatcactt tgactgcgcc cgatcaatat ggcaaatatg ttggcatctg gcagctcaag
     7021 aatgcacagg gcaacttcgg tacgtcgatg agtgcgatca tcgtcgcagg cgagccgcct
     7081 gccggcacgg tcgttgcctc cgcaccggcc gcaccgatcg gcaacattgg caatttcgag
     7141 atcggcggac aaattaacgg
//
LOCUS       DASRTJ010000210         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_345858,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000210 DASRTJ010000000
VERSION     DASRTJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345858"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFF70_04460"
     CDS             complement(<1..1196)
                     /locus_tag="VFF70_04460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZY43983.1"
                     /translation="MLKRIGNNFAILLFVGDMLLTVTALALARYFRTILPFGLQIDPI
                     TLEKINFEIDWPFYLLVLVVWAIVFLILPVYNNQRSLHAIGDVQNTVVAIAFAILILS
                     GIDYFFYREFSRVLFVYFILIDAFLLITWRAIIRLALRLQHAAWPQSKRRVLIVGTGY
                     LAEEVATRIRTYAWTGLELIGFLSEAPDSNQPSGIDRTAVLGSLTAAQSIVTQQHIDE
                     ILIALPLRAHQEMIDLITSLQQLPVSVRVVPDLFDLAFARTGIEDIDGIPIVSLRDPA
                     ITPFRRAIKRIFDLIVAWLTLIVCAIPMAVIAIAIRHDSPGPALYRQQRIGENGKLFR
                     MLKFRTMIDGADDQLSELIEKQTNGEVIFKRRDDPRITRVGRRLRQFSLDELPQLFNV
                     ITGEMSL"
     gene            complement(1235..1537)
                     /locus_tag="VFF70_04465"
     CDS             complement(1235..1537)
                     /locus_tag="VFF70_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43984.1"
                     /translation="MTPIDWGEIFSNALWILGCAIALAAFSYADWRADQEHEKLRAQF
                     ARPKFRLAFGLSLVLIGLGLAASSNSIITLIVWLSLTLVFSIHFVADAIKLRRSAH"
BASE COUNT          418 a          402 c          481 g          346 t
ORIGIN      
        1 agactcatct cgccggtgat cacgttgaac aattgtggca gttcgtcaag actgaattgt
       61 cgcagccggc gaccgacacg cgtgatgcgc ggatcatcgc ggcgcttaaa gatcacttcg
      121 ccgttggttt gtttttcaat cagctcagac aattgatcgt ccgcgccgtc gatcatggtg
      181 cggaacttca acatccgaaa gagtttgccg ttttcgccaa tgcgctgctg gcgatagagt
      241 gccgggccgg gcgagtcgtg cctgatcgca atagcgatca ctgccatcgg aatggcgcag
      301 acgattagag ttagccacgc aacgatcaaa tcgaagatgc gtttgatcgc gcgacggaaa
      361 ggtgtaatcg ccggatcgcg cagactgacg atcgggatgc cgtcgatatc ttcgatcccg
      421 gttcgcgcaa aagccagatc gaatagatcg ggcaccacgc gcacactcac gggcagttgt
      481 tgcaggctgg tgatcagatc gatcatttct tgatgtgcgc gcaagggcag ggcgatcaag
      541 atttcgtcga tatgctgctg agttacgatc gattgcgcgg cggttaatga gccgagcacg
      601 gctgtccgat caatgccgct tggctggttc gagtcgggcg cttcactgag aaaaccgatc
      661 agttccaggc ccgtccaggc gtaagtgcga atgcgcgtgg caacttcttc ggctaggtag
      721 cccgtgccga cgattaaaac gcggcgtttg ctctgcggcc aggcggcatg ttgaagacgc
      781 agggccaggc gaatgatcgc gcgccaggtg atgagcaaga acgcgtcaat caatatgaag
      841 tagacaaaca acacgcgcga aaattctcgg tagaaaaaat aatcgatgcc ggacagaatc
      901 aaaatggcga aggcgatagc gacgacggta ttttgaacgt cgccgatggc atgcaaggag
      961 cgttggttat tgtatacggg caagatcagg aaaacgatcg cccaaacgac cagcaccaat
     1021 aaataaaacg gccaatcgat ttcaaaattg atcttctcaa gcgtgatcgg atcgatctgc
     1081 aggccgaatg ggaggatcgt tcgaaagtac cgcgccagcg ccagggcggt aaccgtgagc
     1141 agcatgtcgc cgacgaagag gagaattgcg aagttattgc cgatgcgttt taacatgata
     1201 accgatgacg cgcggagtgt tgatcgctga gtgtttagtg tgcagaccgt ctcaacttga
     1261 tcgcgtccgc gacaaagtga atgctaaaca ctaaagttaa gctgagccag acgataaggg
     1321 tgatgattga attcgaactg gcggctagac cgagaccaat gaggaccaga gataatccga
     1381 aagccaggcg aaacttggga cgtgcgaatt gcgcccgcaa tttttcatgt tcttgatcgg
     1441 cacgccagtc ggcgtagcta aatgcggcta aggcgatcgc gcagcccaga atccatagtg
     1501 cgttggaaaa tatttcgccc cagtcgatcg gtgtcatgct gttaagggat taggtgctga
     1561 gtgggctgaa ttgcgcaata acttcatcga gcgtctggct tagcctgtag tgagtcgatc
     1621 acaatcgcac cgagtacacc caacacc
//
LOCUS       DASRTJ010000211         7842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_315232,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000211 DASRTJ010000000
VERSION     DASRTJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315232"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VFF70_04470"
     CDS             <1..2044
                     /locus_tag="VFF70_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HZY43985.1"
                     /translation="HRPQQVKMLRAVSDAFNEGQHLLVEAGTGTGKSIAYLLPAIHWA
                     VQNGERVVISTNTINLQEQLATKDVPDLQKVLPFEFRAAVMKGRSHYLCPSRLQLVRR
                     NGPTSIEEMRVLAKVLLWLPTTINGDGDELFIPNQAERAVWNRLSADNEACDNERCAE
                     TGCFFFRARQAAESAHVLIVNHALLLADIAVENRALPDYQYLIVDEAHHLENATTQQL
                     SFAISRAALKRMMEDIGRSKRSEATGLLLEVIGRIRSSVPAELADPTQAFAEKIAEGI
                     DGVLRYIDDLFEQLTDFAREYSESKSDYSQRLRLVMGLRHQPAWDNIEMAWENVSVQL
                     FTVIDGLQRLGAALKDMSEYEIPDYEDLVARVTGYVRTLTEVRTKTNAILTQPVSNEI
                     YWLEVEPNQSGRDGGRGSLSLHAAPLHVGPLVDRFLWKVKKSIVLTSATMRTGRDFTF
                     IRDRLAAQDMDELSVGSPFDYKASTLLYLVTDVPEPGEPGFQKAIDLGLITLAKAMGG
                     RTMALFTSYSSLKATAKGITHTLNQSDILVYEQGDGSSRRQLLDNFKGADRAVLLGTR
                     SFWEGIDVQGEALSCLAIIRLPFAVPSDPIFAARSETFEEPFMQYSVPDAILKFRQGF
                     GRLIRSKTDRGVVAVFDKRLLTKQYGQTFLQSLPQCTVRRGSYAELASAAIDWMKK"
     gene            2153..2710
                     /gene="efp"
                     /locus_tag="VFF70_04475"
     CDS             2153..2710
                     /gene="efp"
                     /locus_tag="VFF70_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZY43986.1"
                     /translation="MIDVNALRKGTTFELDGEIFRVLEYAHNKPGRGNATIRVKLRNI
                     RSGSTFEKTFGSGDRVQDIRIESRAMQYLYHDGDLYHFMDADTFEQIALDAKLLEDSV
                     HFLKDGMAVVIQDYQGESLGIELPVNIELKVAQSDMAVRGDTAAGTNKLVTVETGYRV
                     QVPLFVNEGDTIRIDTRTGEYLTRV"
     gene            complement(2806..3057)
                     /locus_tag="VFF70_04480"
     CDS             complement(2806..3057)
                     /locus_tag="VFF70_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43987.1"
                     /translation="MDSGLPAKIEKAIQYAQELDRFEFQQIHVNVRGSHTMHAVEFNS
                     GAWKCDCEFFITHKQCSHTIAVEKVLGLMLPGAIPMLPH"
     gene            3411..4019
                     /locus_tag="VFF70_04485"
     CDS             3411..4019
                     /locus_tag="VFF70_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZY43988.1"
                     /translation="MTITTLFFDVGGVLLTNAWDTAARKRAVDKFRLEWQEFESRHEM
                     LKYSFETGRIRLDEYVQRSIFRTPRDFTPDEFKQFMFQQSQPLPGGLDCVRALKATGR
                     YRLLMLNNESLELNEYRLKTFGLTEYFDDFFSSCYVGRAKPDQDIYRIALQVTHVDPM
                     QAIFIDDRAVNLESAWDLGLHAVRYEGAAQLMEYLAQQGVTV"
     gene            4101..5006
                     /gene="gnd"
                     /locus_tag="VFF70_04490"
     CDS             4101..5006
                     /gene="gnd"
                     /locus_tag="VFF70_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HZY43989.1"
                     /translation="MELAMIGLGRMGGNMARRLVKGGHRVVAYNRSPDPIKEIEKEGA
                     VGAYSLKEVVAKLTAPRVVWIMVPAGKPTDDMIDQLMSLLANGDTIVDGGNSNFHDTM
                     RRAETVKTKGVHFVDSGTSGGIWGLKEGYSLMIGGDNEAVDRLSPIFETLAPAKDKGW
                     GHVGPSGSGHFTKMVHNGIEYGLMQAYAEGFEILKARKDFNLDLCQISEVWRYGSVVR
                     SWLLDLAANALAEDPNLTHIKPYVEDSGEGRWTVFESINEDVAAPVITLSLMMRFLSR
                     QDDSYSFKMLAALRNQFGGHAIKTE"
     gene            5094..6557
                     /gene="zwf"
                     /locus_tag="VFF70_04495"
     CDS             5094..6557
                     /gene="zwf"
                     /locus_tag="VFF70_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZY43990.1"
                     /translation="MPNTRNLPPFSIVIFGVTGDLSHRKLLPALYNLFVQDLLPESFT
                     IVGFARREWDDDIYRSEMRQSVEQFSRVKVDPAMWAKFSPSLHYVRSEFDQAEGYSAL
                     GRLLAALPNEAGMNRLFYLATPPDAYPVIARSLGAASLNETFKGWTRIIVEKPFGRDL
                     QSAVQLNHQLHYVFAEDQIYRIDHYLGKETVQNMIVFRFANAIFEPIWNRNYIDHVQI
                     TVAETVGVESRAGYYDTAGIGRDIFQNHLLQLMTLTAMEPPYAFDEHALRDEKVKVLQ
                     ALRPIQLTDTMPGQYVGYRNEKGVAPKSITPTFGAMKLYIDNWRWQGVPFFLRSGKML
                     ASKTTEISIEFKRPPHSLFPLERGEQLTPNGLTICIQPDEGMHLRFEAKVPGAGMKRQ
                     TVDMDMHYATDFDQAQLPEAYERLLMDAIQGDAALFTRADEIELAWRAVDPVVQAWES
                     KVVPLYFYEPGSWGPFDAVKLLGEDRRWRSSCGEADA"
     gene            6550..7341
                     /locus_tag="VFF70_04500"
     CDS             6550..7341
                     /locus_tag="VFF70_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZY43991.1"
                     /translation="MLDNLIVFEDIVALVHAAAQRFISIGLAAIQSSDRFTVALSGGS
                     TPHALYEILARDDQSFAWDKVHFFWGDERCVPPDHAESNFGLAHRVLLTKVNVLEQNV
                     HRIHGEENPQQAAIGYEHELRKVFGEQLKPNEPQSRSVAARAMSSGGIDLFPRFDLIL
                     LGLGADGHTASLFPQTTAIHEQRRWVIANHVEQLHTDRITLTPPVINRAQQIIFLIAG
                     SDKAPALRSVLQGQRDPNQYPAQIVKPVSNGLTWLVDRAAATFLA"
     gene            7416..7835
                     /locus_tag="VFF70_04505"
     CDS             7416..7835
                     /locus_tag="VFF70_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY43992.1"
                     /translation="MLAGMESPHSAVAAQPILVALMCENPIVYQSLSSRLAAAEAIHV
                     VDAIDCQLDNVPLVVAENPDVVILGIARITHFNLLVCQAIRQASAQTRIVVLPSYEAD
                     PIELQEAREAGASAILLKNIDTPKLVDQIHALVNSSA"
BASE COUNT         1777 a         1976 c         2212 g         1877 t
ORIGIN      
        1 gcatcgaccc caacaagtca agatgctgcg tgccgtatcg gacgcattca atgaaggaca
       61 acatctgttg gttgaagcag gcacgggcac cggcaaatcg atcgcgtatc tgctgccggc
      121 gatccattgg gcggtacaaa atggcgagcg cgtcgtcatc tctaccaata ccatcaactt
      181 gcaggagcag ctggcgacga aagatgtgcc tgatttacaa aaagttttgc cgtttgaatt
      241 tcgcgcagcg gtgatgaagg gccgatcgca ttatctgtgc ccgtcgcgct tgcaattggt
      301 tcgccgcaac ggtccgacca gcatcgaaga gatgcgcgta ctggcaaagg ttttgttgtg
      361 gctgcccacg acgatcaacg gcgacggtga tgaattgttc attcccaatc aagccgagcg
      421 cgccgtgtgg aatcgcttga gcgcggataa tgaagcctgc gataacgagc gctgtgcgga
      481 aacgggctgc ttcttctttc gcgcgcggca agccgctgaa tcagcgcacg tgctcatcgt
      541 caatcacgcc ttattgttgg ctgacatcgc ggttgagaat cgcgcgctgc cggactatca
      601 atatctcatc gtcgacgaag cgcatcatct ggaaaatgcc acgacgcagc agttgagttt
      661 cgccatcagc cgcgctgcgc tcaagcgcat gatggaagac atcggtcgat cgaaaagaag
      721 tgaagccact ggcttgttgc tcgaagtgat cggccgcatc cgatcgtctg tgccggccga
      781 actggccgat ccgacgcaag ccttcgccga gaaaatcgcg gaaggcatcg atggcgtgct
      841 gcgctacatc gacgatctct ttgagcagct cacggatttt gcgcgcgaat attcggaaag
      901 caagagcgac tactcgcagc ggctgcgctt ggtgatgggc ttgcggcatc aaccggcgtg
      961 ggacaacatc gagatggcgt gggagaatgt cagtgtgcag ttgttcaccg tgatcgatgg
     1021 cttgcagcgt ttaggcgccg cgctcaaaga catgagcgaa tatgagattc ctgattacga
     1081 agatctggtc gcgcgcgtaa cgggttatgt gcgcaccctc accgaagtgc gcaccaaaac
     1141 aaatgcaatt ctcacgcagc cggtttcgaa tgaaatctac tggttggaag tggaaccgaa
     1201 tcaatcgggt cgcgacggcg gtcgcggatc gttgagcctg cacgccgcgc cgctgcatgt
     1261 agggccgttg gtcgatcggt ttttgtggaa agtcaagaaa tcgattgtgc tgacatccgc
     1321 caccatgcgg accggccgtg acttcacctt tatccgcgat cgattagcgg cgcaggatat
     1381 ggatgaactt tcggtcggct cgccattcga ttataaagcg tccacgctgc tgtatctggt
     1441 gaccgatgtg ccggaaccgg gcgagccggg tttccaaaaa gcgatcgatc taggcttgat
     1501 cactttagcg aaagcgatgg gcggtcgcac gatggcgttg ttcacatctt attcatcttt
     1561 aaaagcgacc gcgaaaggta ttacgcacac gttgaatcag tctgatattt tggtctacga
     1621 gcagggcgat ggatcgtcgc ggcggcagtt gctggataac ttcaagggcg ccgatcgagc
     1681 ggtcttgctg ggaacccgat cgttttggga aggcatcgat gtgcagggcg aagcgctgtc
     1741 gtgtttggcg atcattcgtc tgccgttcgc cgttccgtcc gacccgatct ttgccgcacg
     1801 atcggaaaca ttcgaagaac catttatgca gtactccgtg cccgacgcga tcttgaaatt
     1861 ccgccagggc tttggccgct taatccgctc gaagaccgat cggggtgtgg tggccgtgtt
     1921 tgataagcgg ctgctcacta agcagtacgg tcagaccttt ttgcaatcgc tgccgcagtg
     1981 caccgtgcgg cgcggatcgt atgccgagct ggccagcgcc gcgatcgatt ggatgaagaa
     2041 atagtgtatg taggacaaga tgatatcttg tcccataatg ggttgaccca actccatccg
     2101 attggtataa tcgtaacatc aacattacac agtcaggagt ttttgtttca ccatgatcga
     2161 tgtcaatgct ttgcgtaaag gcaccacatt cgagttggac ggcgaaatct ttcgggtgct
     2221 ggaatatgct cacaacaagc cgggtcgcgg caacgcgacc attcgcgtca agcttcgcaa
     2281 tatccgcagt ggatccacct ttgagaaaac atttggatcg ggtgatcgag tgcaggacat
     2341 tcgcatcgaa agccgcgcga tgcagtactt gtatcacgac ggtgatctgt atcacttcat
     2401 ggatgcagat acctttgagc agatcgcgct cgacgcgaag ttattggagg actcagtcca
     2461 tttcttgaag gacggcatgg cggtggtcat tcaagattat cagggcgagt cattggggat
     2521 tgaattgccg gtgaacatcg agctcaaagt tgctcaatcg gatatggcgg tgcgcggcga
     2581 cacggcggcc ggtacgaata aacttgtcac ggttgagacc ggttatcgcg ttcaagttcc
     2641 gttgttcgtg aatgaaggcg ataccatccg catcgacact cgcactggcg aatatttgac
     2701 gcgcgtttga tgtggtgggg tgaggctggt actcgcccaa tcctgatacg aagcaaaaac
     2761 gggcgaggtg ttcacctcgc ccgtttcgtt ttattaaatt gtcagttagt gcggcagcat
     2821 gggaatggcg ccgggcagca tgaggcccag caccttctcg acggcgatag tatggctgca
     2881 ctgtttgtgc gtaatgaaga attcgcaatc acatttccac gcgccgctgt taaattcaac
     2941 ggcatgcatg gtatgcgagc cgcggacgtt gacgtgaatt tgttgaaact caaaccgatc
     3001 aagttcctgc gcgtattgaa tagccttttc gatcttcgct ggcaagcctg aatccataac
     3061 tgcctccttg agggatgatt ggggtaaccg tgcgacaaaa agcacgacgc ccgttaaggc
     3121 catcgtgctt tcaaccgtga gactgaattg caactcgacc gcagcttgct gtgtgcaagg
     3181 ggccaacctc catctcaaat ctagtgatag tatagggcaa gatcggtctg cttgtcaacc
     3241 cctgaattgc caatttattc cgacgattgc catacgcttt tcagtgttac gttgattcta
     3301 aaccgattgg ggtacagtca gcggatttga cggagaggtt ctatcagtat tatactgatt
     3361 gcagccaccc acttagccaa aactacttaa gcggctcaac tttaataaag atgacaatta
     3421 ctacattgtt tttcgatgtc ggcggtgtgc tgctgaccaa tgcctgggat actgcagcgc
     3481 gcaaacgtgc ggtcgataaa ttccgattgg agtggcagga atttgaaagc cgccacgaga
     3541 tgctgaagta ttccttcgaa accgggcgca tccgattgga cgagtacgtt cagcgatcga
     3601 tcttccggac cccgcgcgat ttcacaccgg atgaattcaa gcagtttatg tttcagcagt
     3661 cgcagccctt accgggcgga ttggattgtg tgcgggcatt aaaagccacc ggccgttatc
     3721 ggttgttaat gctgaataat gaatcgcttg aactcaatga gtatcgcttg aaaacttttg
     3781 ggctgacgga atacttcgac gattttttta gttcatgtta tgtgggtagg gctaaacccg
     3841 atcaggatat ttaccgcatt gcgctgcagg tgacgcacgt cgatccgatg caggcgatct
     3901 tcatcgatga ccgcgccgtc aatctggaaa gcgcgtggga tttgggactg catgcggttc
     3961 gatatgaagg cgcggctcag ttgatggaat atctcgccca acaaggcgtt acggtttgac
     4021 aatgataatt gaccaaatga gttacgcact acgtaatacc cagttgaatt tacgcatcag
     4081 tctgagagga gacgaataac atggaactag caatgatagg cttgggtcgt atgggaggca
     4141 acatggcgcg ccgtttagtt aaaggcggcc atcgagtggt ggcgtacaat cggagtcccg
     4201 atccgatcaa agagatcgaa aaagaaggtg cggtcggtgc gtattcgctg aaagaagtcg
     4261 tcgcgaaact gaccgcgccg cgcgtggtgt ggatcatggt gccggccggc aaaccgaccg
     4321 acgatatgat cgatcagctg atgagcttac tagctaatgg cgacacgatt gtcgacggcg
     4381 gcaattcgaa ttttcacgac accatgcgcc gcgccgaaac ggtgaagacg aaaggcgtgc
     4441 acttcgtcga ttcaggcacc agcggtggaa tttggggctt gaaagaaggg tatagcttaa
     4501 tgatcggcgg cgataatgaa gcagtcgatc gcttgtcccc gatctttgag actttagcgc
     4561 ccgcgaagga taagggctgg ggacatgtgg ggccgagtgg atcaggtcac ttcacgaaga
     4621 tggtgcacaa cggcattgag tatggcctga tgcaagctta cgccgaaggc tttgagattt
     4681 tgaaagcgcg taaagatttc aatcttgatc tgtgccaaat ttcggaagtc tggcgttacg
     4741 gcagcgttgt tcgatcgtgg ctgctggatt tagccgctaa tgctttggcc gaagatccga
     4801 atttgacgca catcaagccg tatgtcgagg attccggcga aggacgctgg acagtttttg
     4861 aatcgatcaa cgaagatgtg gccgcgccgg tcataacctt gtcgttgatg atgcgcttcc
     4921 tcagccgcca ggacgattcg tattcgttca agatgctggc cgcgctgcgt aatcaattcg
     4981 gcggacacgc cattaagact gaatgacaaa ttacaaatga ataatgacaa atgaagaact
     5041 gcgccatgct ggtttagtca tttgtcattt gctcatttat cattgagacc attatgccta
     5101 acacccgcaa ccttccgcct ttctcgatcg tgattttcgg cgtcaccggc gatctcagcc
     5161 atcgcaaact gctgccggct ttgtataact tattcgtgca ggatttgctg cccgagagtt
     5221 tcaccatcgt cggttttgcg cggcgcgaat gggatgacga tatatatcga tcggaaatgc
     5281 gccagtccgt tgagcagttc tcacgcgtca aagtcgatcc ggcgatgtgg gctaaatttt
     5341 cgcccagcct gcactatgtg cgatcggaat tcgatcaagc ggaaggttat tcggccttgg
     5401 gtcggctgct ggcggctctg ccaaatgagg ccggtatgaa ccggttgttt tatctggcga
     5461 ccccgcccga tgcttatccg gtcatcgccc gcagtttggg cgcagccagt ctcaatgaaa
     5521 ctttcaaagg ctggacgcgc atcatcgtcg agaaaccgtt cgggcgcgat cttcaatcgg
     5581 cggtgcagct taaccaccaa ttgcattacg tgttcgccga agatcagatc tatcgcatcg
     5641 atcattatct gggcaaagaa accgtccaga acatgatcgt gtttcgcttt gccaatgcca
     5701 tctttgagcc gatctggaat cgcaactaca tcgatcatgt gcagatcacc gtggccgaaa
     5761 cggttggcgt tgaatcgcgc gccggttatt acgacacggc cggtattggc cgcgatatat
     5821 ttcagaacca tctgctgcag ttgatgacgt taacggccat ggagccgcct tacgcgtttg
     5881 acgagcacgc gctgcgcgat gaaaaagtaa aagtgctgca agcgctgcgg ccaattcaac
     5941 tcactgatac gatgccgggt caatatgtgg gctaccgcaa tgaaaaaggc gtggcgccca
     6001 aatcgatcac gccgactttt ggcgcgatga aattgtatat cgacaattgg cgctggcagg
     6061 gcgtgccgtt tttcttgcgc tccggcaaaa tgctggcgtc gaaaacgacc gagattagca
     6121 ttgagttcaa gcggccgccg cattctttgt ttccgctgga gcgcggcgag cagctgacgc
     6181 ccaatggcct gaccatttgc attcagccgg atgagggcat gcatttgcgc tttgaagcca
     6241 aagtgcccgg cgcgggtatg aaacgtcaaa ccgtggatat ggatatgcac tatgctaccg
     6301 acttcgatca agcgcaactg cctgaagctt acgagcgctt attgatggat gcgattcaag
     6361 gcgatgccgc gctgttcaca cgcgccgatg agatcgaact ggcctggcgc gccgtcgatc
     6421 ccgtagtgca ggcctgggaa tcgaaagtgg tgccgctgta tttttacgag ccgggttcat
     6481 ggggcccgtt cgatgctgta aaattattgg gcgaggatcg ccgctggcgc agttcgtgcg
     6541 gtgaggcaga tgcttgataa tctgatcgtc tttgaggata tcgttgcgct ggtgcatgcg
     6601 gctgcgcagc gcttcatttc gatcggtttg gcggcgatcc aaagttcaga tcgattcacc
     6661 gtcgcgttgt ccggcggatc aacgccgcat gcgctgtatg aaatcctcgc gcgcgacgat
     6721 caatcttttg cgtgggacaa agttcatttc ttttggggcg atgagcgctg tgtgcctccc
     6781 gatcatgccg agagcaactt cggcttggca cacagggtgc tgttgaccaa agtgaatgtg
     6841 cttgagcaaa acgtgcatcg gattcacggc gaagagaatc cacaacaagc tgccattggt
     6901 tatgaacatg aattacgcaa agtttttggt gaacaactca aacctaatga gcctcaatcc
     6961 aggagcgtag cagcacgggc aatgtcatca ggtggcatcg atttatttcc gcgcttcgat
     7021 ctgatcctgt tgggcctggg cgcggatggg catacggctt cgttgtttcc gcagaccact
     7081 gccatccatg agcagcgccg ctgggtgatc gccaatcacg ttgagcagct gcacaccgat
     7141 cggattacgc tcacaccgcc tgttattaat cgcgcccagc agattatctt tttaattgcg
     7201 ggcagcgaca aagcacctgc gcttcgatcg gttttgcaag gtcagcgtga tcccaatcaa
     7261 taccccgcac aaattgtgaa gcctgtttcg aacggcctaa cgtggctggt ggatcgggct
     7321 gcggctacct ttctggccta attccagcca gcgtgtgtca ttcatcaccc cggcgatatg
     7381 caatcttgca ctaaacgcgc cgaagcgatg tctgtatgct tgcgggtatg gaatcgccgc
     7441 attctgcagt agctgctcaa ccgatccttg tggccttgat gtgcgaaaac ccgatcgtct
     7501 atcaatcgct gtcgtcgcgt ctcgcggccg ctgaagccat tcatgtcgtc gatgcgatcg
     7561 attgccaact cgacaacgtg ccgctggtcg tggccgaaaa tcccgatgtg gtcattctgg
     7621 gtatcgcgcg cattactcat tttaatctgt tggtctgtca ggccattcgt caggccagcg
     7681 cgcagacccg cattgtcgtg ctgccttcgt atgaagccga cccgatcgaa ctgcaagagg
     7741 cacgcgaggc cggcgcgtcc gcgatcttat tgaagaatat cgatacgccc aagctggtcg
     7801 atcaaatcca cgcgctggtc aattcttccg cttaagcgtt aa
//
LOCUS       DASRTJ010000212         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_345877,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000212 DASRTJ010000000
VERSION     DASRTJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345877"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VFF70_04510"
     CDS             complement(<1..1267)
                     /locus_tag="VFF70_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY43993.1"
                     /translation="MENSMIAVNVTKVNLDLFARPILIDLDWEIHADRCVGLIGANGA
                     GKSSLLKLIAAELQLDSGSITRSKGLTIGYLAQEPALDPDRTALAEALSALEELHVIE
                     ADLQKVEAKLSDPSVYGDEKTLARVLDQQHHLLQRFEELGGANYGSRVRDILRGLGLS
                     ETDFQNTVAALSGGQKKLVGLAKLLITQPDLLLLDEPDNHLDLAGKAFLERTINAYHG
                     AVVIVSHDRYLLDQVVDEIAELEAGRITTYPGSYSEYAYEKQTRLARQQQLYGIQQRQ
                     IDRLQSAAKQLLIWGRQYDNPKLIKRGKAILSRIEKIDQIDRPILEPKRMNLQINGWR
                     GSKKVLEIKGLAKSFVQHPERSAEVEDISTQAKDAVSPKDAPSTPLRTQQVRNSAQDA
                     SISLFSDLNLLIRHGERVGLVGPNGAGKSV"
     gene            complement(1500..2726)
                     /locus_tag="VFF70_04515"
     CDS             complement(1500..2726)
                     /locus_tag="VFF70_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY43994.1"
                     /translation="MSKPLRLLLLMADTGGGHRAASQALAEGLRHRYGDAVDPVLFDG
                     LRAYAPFPVSHLDDMYPFMTSFRKTWGKSYSALNEPTRAQRFMKSWWPYVRTSALHMA
                     QEPCDAIVCVQPLYVYPVLWAMDHIGIRKPFITMVSDLIVVHALWCDPTTDAFLVPTD
                     LSYKHALENLIPEEKISITGLPIRLLFTQPPEPKPVVRARLGLKPDKPMVLMMGGGQG
                     LGRVYEIAEAIGQSGLNLQLVVVAGRNKKLKEQIDGANWPTHSGKDPQGRTVDLRGYG
                     YTEEIPALMNAADILITKAGPTTICEAFTRSLPMIISSYIPVQEEENAEYVTRHGAGA
                     YTEEPEKIVEILRQWLSDPALLERMSKAAGVLARPRAAIEAAEAVYRAATSKPIITSA
                     PQREPLLARIDRLLGL"
BASE COUNT          716 a          890 c          879 g          767 t
ORIGIN      
        1 ccactgattt gcccgcgcca tttggcccga ccagccccac tcgttcgcca tgacggatga
       61 gcagattgag atcgctgaat aaagagattg aagcatcctg agcggaattg cgaacttgtt
      121 gagttcgcaa tggagtcgaa ggagcatctt ttggtgaaac cgcgtccttc gcctgcgtgc
      181 tgatgtcttc gacttcagca ctccgctcag gatgctgaac gaaacttttt gctaaaccct
      241 ttatctccaa aacttttttg ctgccccgcc aaccattgat ctgcaagttc atgcgctttg
      301 gctcgagaat tggccgatcg atctgatcga tcttctcgat gcggctgaga attgccttgc
      361 cgcgtttgat caacttcgga ttgtcgtatt ggcgacccca gatcagcagt tgtttagccg
      421 cgctttgcaa tcgatcgatc tggcgctgtt gaatgccgta caactgctgc tgacgcgcca
      481 ggcgggtttg cttctcatag gcatactctg aatacgaacc gggataagtg gtgatgcggc
      541 ctgcttccag ttcggcgatc tcatcaacca cctggtcgag cagataccga tcgtgcgaaa
      601 cgatgacgac tgcgccgtga taagcgttga tcgttctttc caaaaatgct ttaccggcaa
      661 gatcgaggtg gttatccggc tcgtcgagca gcagcagatc cggctgggtg atcagtaatt
      721 tagccaggcc tactagtttc ttttgaccgc cgctcaacgc ggctactgtg ttttgaaaat
      781 cggtctcgct taagccgagg ccgcgtaaga tatcgcgcac ccggcttcca taatttgcac
      841 cgcccaactc ttcaaagcgt tgtaagagat gatgctgttg atcgagcaca cgcgctaaag
      901 ttttttcatc cccatatacg ctgggatcgc ttaacttcgc ttccaccttt tgcaagtccg
      961 cttcgatgac gtgcaattct tccaaggccg atagtgcctc agcgagcgcc gtgcgatccg
     1021 gatcgagtgc gggttcttgg gccaggtagc cgatggtcag ccctttcgat cgggtgatag
     1081 agccactatc gagttgcagc tcggccgcga tcaatttcag cagtgatgat ttccccgcac
     1141 cgttcgcgcc gatcaaaccg acgcagcgat ccgcatgaat ctcccaatcg agatcaatca
     1201 agatcgggcg ggcgaacaaa tccagattta ctttagtcac atttaccgcg atcatgctat
     1261 tttccattca agcctcggcc tggcaaggcc atggctgttt ataatcaaaa acaaaaaacc
     1321 gcagggcgcg cctgcggttg agaatagtac gacaagctga ccacttgtct taccgacgac
     1381 gaggcgcaat acgataaggc gagggcgcaa tcgggccgcg cgtcgcggtg cagacgataa
     1441 tcgtattggc agtcaggaaa tacattgtgg cctgattata acggcggttg aagaatacgt
     1501 caaagtccaa gcaatcgatc gatgcgggcg agcaggggtt cgcgttgagg cgcgctagta
     1561 atgatcggtt tggatgtggc cgcgcgataa acggcctcgg ctgcttcgat cgccgcgcgc
     1621 ggccgagcca gcacgccagc agcttttgac atgcgttcga gcagggcggg atcagacagc
     1681 cactgccgca atatttcgac gatcttctcc ggttcttcag tgtatgctcc ggcgccgtgc
     1741 cgcgtcacat actcagcgtt ttcttcttct tgaaccggaa tataactgct gatgatcatc
     1801 ggcaggctgc gggtaaaggc ttcgcagatc gtcgtcggcc cagccttggt gatcaagata
     1861 tcggcggcat tcatcagcgc cgggatttct tcggtgtagc cataaccgcg cagatcgacg
     1921 gtacgcccct gcgggtcttt accgctgtgg gtcggccagt tggccccgtc gatttgttcc
     1981 tttaattttt tgttgcggcc cgccacgaca accaattgca ggttgaggcc gctctgcccg
     2041 atcgcttcgg cgatctcata cacgcggccc agcccttgac cgccgcccat catcaacacc
     2101 atgggcttgt cgggcttgag acccaatcgg gcgcggacga ccggtttcgg ttcgggcggc
     2161 tgcgtgaaca acaatcgaat tggcaaaccg gtgatcgaaa ttttttcttc ggggattaaa
     2221 ttttccagcg cgtgtttgta actgagatcg gtcggcacta aaaatgcatc ggtcgtggga
     2281 tcgcaccaca acgcatggac gacaatcaga tcgctgacca tggtgatgaa cggtttgcga
     2341 atgccgatat gatccatcgc ccataatact ggatagacat acagcggctg gacgcagacg
     2401 atagcatcgc acggttcctg cgccatgtgt aatgcagacg tgcgcacata cggccaccat
     2461 gatttcatga accgctgcgc tcgcgtcggt tcattgagcg cgctgtaact cttgccccac
     2521 gtttttctaa aactggtcat gaacggatac atatcgtcga ggtggctgac tgggaagggc
     2581 gcgtaggccc gcaagccgtc gaacaacacc ggatcgaccg catcaccata acgatgacgc
     2641 agtccttcgg ccaaggcttg ggatgcggcg cgatgtccgc cgccggtatc ggccatgagg
     2701 agcagcagac gcagaggttt actcataagg cgacatgatc aacactgcaa gatcgatgcg
     2761 gttgcgatta taccagaaag agaggcttga tgagtagtgc gtgcgatcga ttgaaaccga
     2821 tccgtcattg cgagcgcagt tcctagatgg cattgcctct agggatgtgc cacgaagcaa
     2881 tctcgttgga ttagtgaaac cgagtgggca tcgcggcgat tttgttcgcg gcctcgtcgg
     2941 tgttctagcg atttgccgtc cgtcattaca gttcagcgag ttcgtgaagt gctgacacac
     3001 tgacaagatg acaaggtgac gttcgtcgat agtcgtgctg agcgaaatga tatgccaaat
     3061 gccacacagc acacaatcta catcatcctt tgcgcaatac gctaaaaacc tcgctctcca
     3121 ctcaacaatt cttccagcgc atcttttggc aattcagcca gcgaacgtac aatgcgatct
     3181 gccggtaacg acgcgtgctt cacgccgacg ccgatcgagt gcatactggc gcgctgtgcg
     3241 ccttcgatgc ca
//
LOCUS       DASRTJ010000213         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_270374,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000213 DASRTJ010000000
VERSION     DASRTJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270374"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3145)
                     /locus_tag="VFF70_04520"
     CDS             complement(<1..3145)
                     /locus_tag="VFF70_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HZY43995.1"
                     /translation="MFNRILSSLILLSIILSACNTTPAPTSTPATIATNVPSPTATPQ
                     GEEPLYLSIVWHHHQPLYYQDPATGIYTRPWVRVHATKDYFDMASTVAKYPNVHVTFN
                     LTPVLIRQLDDFAAGAKDIYWTTAEKPADQLSDDDKRFILQRFFDTNPKIVARFPRYQ
                     ALADKRKSAGPEAIDAALQSFTTQDFRDLQVLFNLAWFDPDFLAQEPLKLLVAKGSNF
                     AESDKQPIFDKALEVIKQVIPEYKNLQDKGQIEVITTPYAHPILPLLYDTNLEKVGNT
                     EAVMPDRYSFPNDAIAQLKKSVEIYQAHYGRAPRGLWPGEGAVAQEIVKMVGDAGYQW
                     MATGEDVLAKSLGLNGFTRDSKDTVQEADQLYQPYVVENKQFGVKVNMIFRDLRLSDL
                     IGFEYSQTPGKQAADDLMQRLENIRQGLKKENATGPHLVSIILDGENAWENYDNDGKD
                     FLNALYQNLSDSKTVKAVTPTEYFTLHPPTQKIDQLFPGAWFSANYDTWIGEEEEATA
                     WNYLLKTRETLYDYDVSKKKSIAPDKLARALDFMYLAEGSDWFWWYGADQNSGDDAYF
                     DRGYRALLAEVYKSLDLNVPEFVQVPIVSANVATPSQQPNGLISPTIDGKVSSDAEWK
                     SAGAYVAAGGAQARSADVASAFYYGLDAKNISFRVDAKSDWKSIGNASIGFYLAQPKV
                     KNKSAFTLFSANQATKNVLGFQATHLLQIDLKNGVVSGAKLYTSDGQNGWEETSPHPK
                     VFTASGGSILEAALPLSTFTDFETGDTFNLVAVVSQDQHDLQSIPSAGLAQVVIPDLG
                     TTQMVLSVDDPVGDDHGSGKYTYPTDGVFKPGVFDIKHFEVGYDDKHLAFKITFAGPI
                     PNPWGSPNNLAVQTLDVYIDKDPGAGTGARKLLPGRNASLPKGDGWDVAVWAEGWTPG
                     VFTPDADGNPKHVDNLDFKVLVDAGQQQVTLRVPLSVFGDNFDPTKAGYTVMVMSQDG
                     FPAPGVWRIRDVDPTASQWRLGGSSGDINHTRIIDLVQPVEAKPTQEEALSKYASAVS
                     GDLDQLTPDDFALVPVLT"
BASE COUNT          767 a          901 c          856 g          817 t
ORIGIN      
        1 ccgttaatac cggcaccaag gcaaaatcat ctggcgtcag ctgatccaga tcgccgctaa
       61 ctgcagatgc atatttgctc aacgcttctt cttgtgtcgg cttcgcctcg acgggctgca
      121 ccagatcgat gatgcgcgta tgattgatgt cgcccgacga tccgcctaaa cgccattgac
      181 tcgccgttgg atcgacatca cgtatccgcc agacaccggg cgcagggaaa ccatcctgac
      241 tcatcaccat caccgtgtaa cccgctttcg tcggatcgaa gttgtcgccg aacacactta
      301 gcggcacacg cagcgtcact tgttgttgac cggcatcgac taagactttg aaatcaaggt
      361 tatcgacatg cttcggattg ccatcagcat ccggtgtgaa aacgcccggc gtccaacctt
      421 cagcccacac cgccacatcc cagccatcac ctttcggcag tgaggcgttg cgtccgggca
      481 ataacttacg cgcgcctgtg ccagcgcccg gatctttatc gatatacaca tcgagcgttt
      541 gtacagccag attattcggc gagccccatg gatttggaat tggtccagca aatgtaatct
      601 tgaaagccaa gtgcttgtca tcgtatccga cttcaaagtg tttgatatcg aacacgcccg
      661 gtttgaacac accgtccgtt ggataagtat atttgcccga cccgtgatca tcaccgaccg
      721 gatcatcgac gcttaagacc atttgcgttg tgcccagatc aggtatcacc acttgcgcca
      781 aacccgccga tgggatcgat tgcagatcat gttgatcttg actgacgacc gcgactaaat
      841 tgaaagtgtc gcccgtttca aaatcggtga aggttgataa cggcaaagcc gcttccagaa
      901 tcgaaccgcc agaggcagta aaaactttgg ggtgtggact ggtttcttcc cagccgtttt
      961 gtccgtcaga ggtgtacagc tttgcgcctg agacgacacc gttctttaga tcgatctgca
     1021 gcaaatgcgt ggcctgaaaa cctaacacgt tcttggtggc ttgattggcg ctgaataatg
     1081 taaaggccga tttgttcttg acctttggct gagccagata aaatccgatc gaggcattcc
     1141 cgatcgattt ccaatccgat ttggcatcga cgcggaacga aatgttcttc gcatcgaggc
     1201 cgtaataaaa cgcggaggcg acatcagccg atcgggcttg cgcgccgcct gctgcaacat
     1261 atgcgcccgc cgatttccat tcggcatccg atgagacttt gccatcgatc gtcggactga
     1321 tcaaaccgtt gggctgttgc gaaggcgtgg cgacgttggc tgaaacgatc ggcacttgca
     1381 caaattccgg cacattaaga tcgagcgact tataaacttc agccaataga gcgcgatagc
     1441 cgcgatcgaa atacgcgtcg tcgccgctgt tttgatcggc accataccac cagaaccagt
     1501 ccgagccttc ggccagatac atgaagtcga gtgcgcgcgc caacttgtcc ggcgcgatcg
     1561 atttcttttt gctgacgtca tagtcgtaca acgtttcgcg cgtctttaat aaataattcc
     1621 aggcagtcgc ctcttcctct tcgccgatcc acgtgtcgta gttggcactg aaccatgcgc
     1681 cggggaacag ctgatcgatc ttctgcgtcg gcggatgaag tgtgaagtat tcagttggcg
     1741 tcacagcctt gactgttttg ctgtccgaca agttttgata gagcgcgttc agaaaatctt
     1801 taccgtcgtt gtcgtagttc tcccacgcgt tctcgccgtc gagaatgatc gagacgagat
     1861 gcgggccagt cgcattttct ttcttcaaac cctgccggat gttttccagg cgctgcatca
     1921 aatcatcggc ggcctgtttg cctggcgtct gtgaatattc aaagccgatc aaatccgaca
     1981 agcgcaaatc gcggaagatc atattgacct tcacaccgaa ctgtttgttc tcaaccacgt
     2041 aaggttgata caactggtcg gcttcttgca cggtgtcttt gctgtcgcgc gtaaaaccat
     2101 tgagaccgag tgacttggct aacacatctt cgcccgtcgc catccattga taacccgcat
     2161 cgccgaccat cttgacgatc tcttgtgcga ctgcgccctc gcccggccac aagccgcgcg
     2221 gcgcgcgacc gtaatgtgct tgataaattt cgacgctctt cttcaactgc gcaatcgcat
     2281 cgttgggaaa cgaataccga tcgggcatga cggcttcggt gttgccgacc ttttcaagat
     2341 tcgtatcgta caacagcggc aagatcggat gggcatatgg cgtggtgatg acttcgatct
     2401 ggcctttgtc ctgcaaattc ttgtattcgg gaatgacttg cttgatcact tccaatgctt
     2461 tatcgaagat cggttgctta tcgctctcag caaagttact acccttggca acaagcaact
     2521 tcaacggctc ttgcgcgagg aaatccggat cgaaccacgc gaggttgaac aagacttgca
     2581 gatcgcggaa gtcttgggtc gtgaaagact gcagcgccgc atcgatcgct tctggaccgg
     2641 cgctcttgcg tttgtcggcc aaggcttgat agcgcgggaa gcgcgcgaca atcttcgggt
     2701 tcgtatcaaa gaagcgctgc aagatgaaac gcttgtcatc atcgctcaat tgatcggcgg
     2761 gcttctcagc agttgtccaa taaatatctt ttgcgccagc ggcaaaatcg tcgagttggc
     2821 gaatcagcac cggcgtgaga ttgaaggtca cgtgcacatt aggatacttc gccacggtgg
     2881 aggccatgtc gaaataatct ttggtggcat gcacacgcac ccacggtcga gtgtaaatgc
     2941 cggtcgccgg atcttggtag tagagcggtt gatgatgatg ccagacgatc gatagataca
     3001 ggggctcttc accttgcggt gtcgccgtgg gcgacgggac gttcgtggcg atcgtggccg
     3061 gagttgacgt gggcgcaggc gtagtgttgc aggcgcttaa gatgatcgag agcaagatca
     3121 atgaagagag aattcgattg aacatggtta cacctcacta cgaaatgtca tttcgaggcc
     3181 acgagccgag aaatctatgc aacgatcagc aacaattcct aacagtggta aagatttctc
     3241 gcttcgctcg aaatgacaat catatgctta acaccattcc acttctcggc gcaagtttca
     3301 attgatgcag catgccgcca gtcatttgaa ttttgtagct c
//
LOCUS       DASRTJ010000214         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_345946,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000214 DASRTJ010000000
VERSION     DASRTJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345946"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFF70_04525"
     CDS             <1..364
                     /locus_tag="VFF70_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003216509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZY43996.1"
                     /translation="VYMGWLLWRSDPLSDDQTRDAKQWPARRQIIFALSVSLLNPHAI
                     LDTIGVIGTSSLAYVDEAKFAFTLACVLNSWLWFFVLAVVGRWVGSIVRVRRVMNRVS
                     AIIMWLSAIYLVYNLTKV"
     gene            509..892
                     /locus_tag="VFF70_04530"
     CDS             509..892
                     /locus_tag="VFF70_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY43997.1"
                     /translation="MDKLGVKPMSRVSVIGIEDRSFWQLLIDSGAEAIKGRLRKDSDL
                     IFYAADSLAALKQLDRLKSYLKANGAIWVVSLKGNGAKIKDIDVIAAAKRSGLVDNKV
                     VSFSETHTSLRLVIPLKQRNKQSTN"
     gene            962..1522
                     /locus_tag="VFF70_04535"
     CDS             962..1522
                     /locus_tag="VFF70_04535"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZY43998.1"
                     /translation="MDRCPWPGNNELMIKYHDEEWGVPLHDDQKLFEFLVLEGVQAGL
                     SWQIVLNKRENYRRAVHDFDPIKIARYTARDVQRLLKNGGIIRNRLKIEAMINNARRF
                     LEVQAEFGSFNQYIWRFVDGKPIANKLKTLKQIPATSKESDALSTDLKQRGFKFVGST
                     IVYAHMQATGMVNDHLIDCFRHDEVG"
     gene            1728..2936
                     /locus_tag="VFF70_04540"
     CDS             1728..2936
                     /locus_tag="VFF70_04540"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZY43999.1"
                     /translation="MPNQSFTLGVNYWPRRKAMTWWSNFDRGEVCEEFAIIREYGMSL
                     ARIFLLWDDFQPEPDQVSHDCLDHLTTVCEVAAEQNLKLDVTFFTGHMSGPNWSPRWL
                     LDDRSRPQARQVVSRGRVTSSGYRNPFTDPIALQAERLLLSTIVKLLKEHPAIGMWNL
                     GNEPDLFAQPPTALQGADWVRTLTELIHSLDGQHPVTCGLHLDSLLNNNGFRVDQVFR
                     ETDVAVMHAYPMYVNWARHPLDREVVPFACALTSALCGKPTLMEEFGGCTTPDRGPSQ
                     MWAWTAFGQPRQQFMASEDDLAQYFETVLPKLIEVGATGALLWCFADYAPELWDRPPC
                     DEARHERFFGLMRPDGSLKMHADIIRKFAETQPIVKPAARSIDLPMSADEFYRAPAVN
                     TAQLYRDYIS"
     gene            3099..>3285
                     /locus_tag="VFF70_04545"
     CDS             3099..>3285
                     /locus_tag="VFF70_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZY44000.1"
                     /translation="MLDLNQVRAHFPALNSGAIFFDNPGGTQVAQETLDRMTRYLQNT
                     NANHGGAFKTSRDSDAIV"
BASE COUNT          761 a          843 c          894 g          787 t
ORIGIN      
        1 ggtttatatg ggctggctgt tatggcgatc cgatccgctc agtgatgatc agacccgcga
       61 tgcaaaacaa tggccggccc gccgacagat catcttcgcc ctgtcggtct cgctgctgaa
      121 cccccatgcg atcctggaca cgatcggtgt catcggcacg agctcgttag cgtatgttga
      181 tgaagcgaag ttcgctttca cgctcgcctg cgtcttgaat tcttggctgt ggtttttcgt
      241 gctggccgtc gttggccgct gggtcggatc gatcgtgcgt gtgcgccgcg tgatgaatcg
      301 cgtctcggcg atcatcatgt ggctgagcgc gatctatctg gtgtacaatt taaccaaagt
      361 gtaggggcga cgcattccac atcaaaatct tgctggatgc caactttatt tcattgagat
      421 gctggtttaa tcacgttcaa ttcggaatgc gtcgccccaa cgggtattgt tcatgatgcg
      481 acccgaattt cattccacgc gcagtctcat ggacaaactc ggcgtcaagc cgatgtcgcg
      541 cgtgagcgtg atcggcatcg aggatcgatc attctggcag ctgctgatcg attctggcgc
      601 ggaggcgatc aaaggccgct tgcggaaaga ctccgatttg attttctatg ctgccgatag
      661 tttagcggcg ttaaagcaac ttgatcgact gaagagttat ctcaaagcca acggcgcgat
      721 ctgggtcgtg tcactcaaag gtaatggtgc taagatcaag gatatcgatg tgatcgcagc
      781 ggcgaagcga tcgggcttgg tggataacaa agtcgtgtcg ttctcggaga cacacacctc
      841 gctccgattg gtgattccat tgaagcagag aaacaaacaa tccacgaatt aacacgaatc
      901 gacacgaaat aatattgatt cgtgaagatc agtgaagatt catggatcaa aaggaatcaa
      961 aatggatcga tgtccgtggc cgggcaacaa tgagttgatg atcaaatatc atgatgaaga
     1021 atggggcgtg ccgctgcacg acgaccagaa attatttgag ttcttggttt tagagggtgt
     1081 gcaggcgggc ttgtcgtggc agatcgtttt gaacaagcgc gaaaattatc gccgagccgt
     1141 gcacgacttt gatccgatca agatcgctcg ctacaccgcc cgcgatgtgc agcggctgtt
     1201 gaagaacggg ggcatcatcc gcaaccgcct gaagatcgaa gcgatgatca ataacgcgcg
     1261 cagattcttg gaagtgcaag ccgaatttgg gtcgttcaat caatacatct ggcgctttgt
     1321 cgacggtaag ccgatcgcga ataaactcaa aacgctcaag caaattccgg cgacctccaa
     1381 agaatcggac gcattgagca cagacttgaa gcagcgcggc ttcaagttcg tcggctcgac
     1441 gattgtgtat gcgcacatgc aagccaccgg catggtcaat gatcatttga tcgattgttt
     1501 ccggcacgac gaggttggtt aaccacagag acacggagaa atataataat agccgcaagg
     1561 atttatactg tcatttcgag cgaagcgaga aatctcatca ccattcaacc ttggtgctaa
     1621 tcgaggtaga gatttctcgt cgcgctgcgc gctcctcgaa atgacaactg aatatcagat
     1681 gtgtaagacg aatctgttaa tcaaactctg tgcctcagtg gtttactatg cccaatcaat
     1741 cttttaccct cggcgtcaat tactggccgc gccgcaaggc gatgacttgg tggtcgaatt
     1801 ttgatcgcgg tgaagtctgc gaagagttcg ccatcattcg cgagtatggc atgtcgcttg
     1861 cgcggatatt tttgttgtgg gacgactttc agccggaacc cgatcaagtt tcgcatgact
     1921 gccttgatca tttaacgacc gtctgtgaag tcgccgccga acaaaatctg aaactcgatg
     1981 tcacgttctt cacgggccac atgagcggcc ccaactggtc gccgcgctgg ttgttagatg
     2041 atcgatcgag accgcaggcc cgccaagtcg tcagccgcgg ccgtgtgacg agcagtggct
     2101 atcgtaatcc cttcactgat ccgatcgcac tacaagccga gcgattgttg ctatcgacga
     2161 tcgtgaagtt gttgaaagag catccggcga ttggaatgtg gaatctcggc aatgagcctg
     2221 atctatttgc acaaccaccc actgctctgc aaggcgccga ttgggtgcgc acgctgaccg
     2281 aactcattca ttcgctcgat ggccagcatc cagtcacgtg tggtttgcac ctcgatagtt
     2341 tgctgaacaa caacggcttt cgcgtcgatc aagtttttcg cgagacagat gtcgccgtga
     2401 tgcatgccta tcccatgtat gtgaattggg cgcgtcaccc gctcgatcgg gaggtggtgc
     2461 cgttcgcgtg tgcgttgacc tcggccttgt gcggcaagcc gacattgatg gaagaatttg
     2521 gcggttgcac cacgcctgat cgcggcccat cgcaaatgtg ggcgtggaca gcgttcggtc
     2581 agccgcgcca acaattcatg gcgtccgaag atgacctggc gcaatatttt gaaacggtgc
     2641 tgcccaagtt aatcgaagtg ggcgcgaccg gcgcgctgct gtggtgcttc gccgattacg
     2701 cgccggagtt gtgggatcga ccgccgtgcg atgaagcgcg gcatgaaagg ttttttggat
     2761 tgatgcggcc cgatggttcg ctcaagatgc acgccgatat cattcgtaaa tttgccgaga
     2821 cacagccgat cgtaaagccc gcagcccgat cgattgatct gccgatgtcg gccgatgagt
     2881 tctatcgtgc accggccgtt aacaccgccc aactttaccg cgattatatt tcttgactga
     2941 tggtcactgg cacttcgagg cttaggccga gaaatctctg cctctgcata cacctgacgc
     3001 cgatcggcgt agagatttct caccgctgcg tggttcgaaa tgacattgcc cgcggcgcac
     3061 attaaccaat tcacaaaaat attctcaagg agactaggat gctcgatctc aatcaagtcc
     3121 gtgcacattt tcctgcgctc aattccggcg cgatcttttt cgataatccc ggcggcacgc
     3181 aagttgcgca ggaaacgctc gatcgcatga cgcgctattt gcaaaacacg aatgccaatc
     3241 acggtggcgc atttaagacc agccgcgact cagacgcgat cgtac
//
LOCUS       DASRTJ010000215         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_88020,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000215 DASRTJ010000000
VERSION     DASRTJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88020"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFF70_04550"
     CDS             complement(<1..287)
                     /locus_tag="VFF70_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44001.1"
                     /translation="MKRFLTLLIIALLTTGCFGEDSSSPIGNPDITPDPSHAAHLPDP
                     VLSPGDTLDVTKDDICVSGYSAKVRDVPQSVKNQVYAEYGITSHAPGSYEI"
     gene            complement(473..1576)
                     /gene="ychF"
                     /locus_tag="VFF70_04555"
     CDS             complement(473..1576)
                     /gene="ychF"
                     /locus_tag="VFF70_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZY44002.1"
                     /translation="MTIRIESGIMTGMQIGIIGLPQTGKTTIFHALAGGEKAASAFSA
                     GRLEIHTAIATVPDDRVDILSEMFKPRKTIYARVTYADIAGLDKGITKKGLPGPFINQ
                     LGQMDAFVHVVRSFDDPAVPHEGPIDPAGDINILDTEFLLNDLGAVERRYEKILESIS
                     KGAKDKELALKEKAIFERLKECLEAEKPLRELDLPHEDIQALRGYGFLTIKPLLIVIN
                     IGDEGAAPKIQYDHKHSAVVVLRGRLEREIAELAPEDAELFMKEYSITELSRSKVIRL
                     SYDLLGLQSFFTVGEDEVRAWTVRRNAFAVEAAAEIHTDLAKGFIRAEVVGYQDLIDL
                     GGMSEAKTKGKFRLEGKEYVVKDGDILHVRHNM"
BASE COUNT          395 a          473 c          485 g          449 t
ORIGIN      
        1 tcgatttcgt atgatcccgg cgcatgcgag gtgataccgt actcagcgta gacttgattc
       61 ttgaccgact gcggcacatc acgcactttg gctgaatagc cggagacgca gatgtcgtct
      121 ttagtcacat cgagcgtatc accgggcgag aggacaggat cgggcagatg cgcagcgtgt
      181 gacggatcgg gagtgatatc ggggttaccg atcggactgc tggaatcttc gccaaagcag
      241 ccagttgtga gcagcgcgat gatgagcaaa gtgaggaagc gtttcataga agcatgtcct
      301 cgttgagcag ggtagaaccg tcattgcgag cgaagcgaag caatctctac tgcaatcagc
      361 tgagtttgct tcggcgcaaa tagaggcgcc tcacaatgac ggctaaatct ctttccatcg
      421 agaaaaagtt tcacttgtga tcaatagcgt attcctaact actcttcact catcacatat
      481 tgtggcgcac atgcaggatg tcaccatctt tgacgacgta ctctttgcct tccaggcgga
      541 acttgccttt agtcttggct tcgctcattc cacccagatc gatcaggtct tgatagccca
      601 cgacttcggc tcgaatgaaa cccttggcca gatcggtgtg aatctcagct gcggcttcga
      661 cggcgaaggc attgcgccgc accgtccagg cgcgcacctc atcttcgccg acggtgaaga
      721 acgattgcaa gccgagcaga tcgtacgaca ggcgaatgac tttgctgcgg ctcaattcag
      781 tgatgctata ttctttcatg aagagctcgg catcttcggg cgcgagttcg gcgatctcgc
      841 gttcgagtct gccccgtaag acgacgaccg cgctgtgctt gtgatcatat tgaatcttcg
      901 gtgcagcgcc ttcgtcgccg atgttgatca cgatcagcag gggcttgatc gtcaagaagc
      961 cataaccgcg cagggcttga atgtcttcgt gcggcagatc gagttcgcgc agcggctttt
     1021 cggcttccag acattccttt aagcgctcga agatcgcctt ctctttcagc gccaactctt
     1081 tgtctttcgc gcctttgctg atcgattcca gaattttttc atatcggcgt tcgaccgcgc
     1141 ccagatcgtt gagcaagaat tccgtgtcca gaatgttgat gtcgccggcg ggatcgatcg
     1201 ggccttcgtg cggcaccgca ggatcgtcaa atgatcgaac gacgtgcaca aacgcatcca
     1261 tctgtcccag ttgattgata aacgggccgg gcaagccttt tttggtgatg cccttatcga
     1321 gtccggcgat atccgcatac gtcacgcgcg cgtaaatcgt tttgcgcggc ttgaacatct
     1381 cgctcaagat gtccacgcga tcatccggca cggtcgcaat cgccgtgtga atttccaggc
     1441 ggccggctga aaacgcagaa gcggctttct cgccgcccgc cagcgcgtga aagatggtgg
     1501 tcttgcccgt ttgaggtaat ccaatgattc cgatttgcat gccggtcatt atgcccgatt
     1561 caatacggat ggtcaagctt gcgtatgtat ccagtcgatt ctataatggt tgagccatcc
     1621 tttagaaatt ttgatcgaaa ggctcaacca taatagactt gtccaccgat gcagcgaagg
     1681 gttgaaaaga ttcatacgga gctgaacgat tattcaaggc gatctcttat gcgcgcaaat
     1741 ataacgaaaa tatttctggt catgctgtta tgctcgatcg ttggggtggc gcccgtggcg
     1801 gc
//
LOCUS       DASRTJ010000216         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_346017,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000216 DASRTJ010000000
VERSION     DASRTJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346017"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFF70_04560"
     CDS             complement(<1..467)
                     /locus_tag="VFF70_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44003.1"
                     /translation="MPRVKGYALPRIIRTRSGIERESRPGTVLVAYSAVETLGRGEAR
                     DIETDLETIDTAKALAKNLREVGYNADTYVVYSMSDIELLAEEFDVDDLLVFNLCEHL
                     NGVAMDDHKLATRMDQLGIQYTGAAPATLRASLNKSRAKRILERHSIPTAPHSL"
     gene            complement(550..1602)
                     /locus_tag="VFF70_04565"
     CDS             complement(550..1602)
                     /locus_tag="VFF70_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44004.1"
                     /translation="MRVALLTNLEENAPHLHTNAPHDALYELEHPHNVEAYKAAIEAH
                     GHQVFPMEGDAFLAQRLKDLAIDVCFNVCEGYRGDAREAQVPALLEMLGYPYSGSKVS
                     TLALTLDKAMTKRVLHYYSLPTPAFQLFETANDPIDSRLHYPLFAKPNSEGTGIGIAG
                     KSIVHNETELRARIAELLEAYHEPVLVERYIEGRDLTVGIVGNGSDLHIFPILEVDKS
                     LFEETGVPVYGYELKVDLADAYHYHCPALLDEPLANELRRLAVEVMRVTNTLDFARVA
                     FRLDESDNNRPYILEINSLPGITPISDLTMMALAEKRTHADLVYSVFEAAMKRLGLQI
                     DPLVLKEKEFVSVRSK"
     gene            complement(1814..>2314)
                     /locus_tag="VFF70_04570"
     CDS             complement(1814..>2314)
                     /locus_tag="VFF70_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HZY44005.1"
                     /translation="LIEGGGHFRTTVSKGIEIIEGLRGHTSGYAVPTYCVDAPGGGGK
                     IPVMPNYMISQAPGKVVLRNYEGFITTYTEPLDYNPHEIDPLDRAARKRIEAGQAGVL
                     GLLEGQRMTIEPQGFGETHLRGAQQHRLNADQSKWQPLGIGTPDRPLLENENVKLIEK
                     DNHEAH"
BASE COUNT          502 a          654 c          597 g          561 t
ORIGIN      
        1 agactatggg gcgcagtcgg aatagagtga cgttctaaga tgcgcttggc acgcgacttg
       61 tttaacgagg cgcgcagtgt ggccggtgcc gcgccggtat actgaatgcc gagttgatcc
      121 atgcgcgtgg ccagtttgtg atcatccatc gcgacgccgt tgagatgttc acacaaattg
      181 aacacaagca gatcgtccac atcgaactct tcagccagca gttcgatgtc ggacatggaa
      241 tagacgacgt aggtgtcggc gttgtaaccc acttcgcgca aattcttagc tagcgccttg
      301 gcagtatcaa tcgtttccag atcagtttca atatcgcgtg cttcgcctcg tcccaatgtc
      361 tcaaccgcgc tataagccac caacacagtg cccggtcgag attcacgctc aatgccgctg
      421 cgtgtccgga tgatccgcgg cagagcataa ccttttactc tcggcatgat ttctcctatt
      481 gccttattcg attgtgatcg atcgggcagg cagcctgaac cccatcggtt ttctgcgcta
      541 aatagtgcgt cacttcgatc gcaccgacac gaactctttt tctttcagca caagcggatc
      601 gatctgcaag ccaagccgct tcatggccgc ttcaaagacg ctgtacacga gatcagcgtg
      661 ggttcgcttt tcagccagag ccatcatcgt cagatcgctg atcggcgtga tgcccggcag
      721 ggaattgatt tccagaatat acggccggtt gttgtcactc tcgtccaaac gaaaagcgac
      781 gcgcgcgaaa tccaacgtgt tggtcacgcg catgacttca acggccaagc ggcgtagttc
      841 gttggctaac ggttcgtcta acagcgcggg acaatgatag tgatacgcat ctgccagatc
      901 gactttcaat tcatagccat acactggcac gcctgtctct tcaaagagag acttgtctac
      961 ttccaagatc gggaagatat gcaaatccga tccattgccg acgatgccca cggtcagatc
     1021 gcgcccttcg atatagcgct caaccaacac cggctcgtga tacgcttcca ataactcggc
     1081 tatgcgcgcg cgcagttcag tctcgttatg cacgatcgat tttccggcga tgccaatgcc
     1141 agtgccttcg ctattcggtt tggcgaacaa cgggtagtgc aagcgtgaat cgatagggtc
     1201 gttcgcggtt tcgaacaatt gaaacgcggg cgtgggcagg ctgtaataat gcagcacgcg
     1261 cttggtcatg gccttgtcca gcgttaaagc cagcgtggac actttcgatc cggagtacgg
     1321 ataacctaac atctccaaca gcgcgggcac ttgcgcttcg cgcgcatcac cgcggtaacc
     1381 ttcgcacaca ttgaaacaca catcgatcgc cagatccttc agccgctgcg ccaaaaaggc
     1441 gtcgccttcc atcggaaaga cttgatgacc gtgcgcttcg atcgcggctt tgtaagcctc
     1501 gacgttgtgc ggatgttcga gctcatataa agcgtcgtgc ggcgcattcg tgtgcaagtg
     1561 cggtgcgttt tcttccaagt tggttaataa agctacgcgc atgagttcac cccctggctc
     1621 gccacatcag ccctctcgaa tttgaaagag agagggcgaa aatttaaact tgaaggatct
     1681 tcagtttcca cagcttatac tccttgattt tttcttgatc tgtcatttcg agcgcggcga
     1741 gaaatctcta taccattcag cgtaaatttc tcatcgcaaa aagatgcgat tcgaaatgac
     1801 aggggtcaat tacttaatgc gcttcgtgat tatctttctc gatcaatttc acattttcgt
     1861 tttcgagcag cggccgatcg ggcgtaccga tgcccaacgg ctgccacttg ctttgatcgg
     1921 cattcaagcg atgctgctgc gcgccgcgca gatgcgtctc gccaaagccc tgcggctcga
     1981 tcgtcatgcg ctgtccttct aacaggccga gtacgccagc ttgtcccgcc tcgatccgtt
     2041 tgcgtgccgc gcgatcgagc ggatcgatct catgcgggtt gtaatcgagc ggctcggtat
     2101 aggtcgtgat gaagccttcg taattgcgca gcaccacttt gcccggcgct tgcgagatca
     2161 tatagtttgg catcaccgga atcttgccgc cgccgcccgg cgcatcgacg cagtacgtcg
     2221 gcacggcgta accgctggtg tgcccgcgca agccttcaat gatctcgatc ccttttgaga
     2281 ccgtggtgcg aaagtggccg ccgccttcaa tcaa
//
LOCUS       DASRTJ010000217         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_50864,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000217 DASRTJ010000000
VERSION     DASRTJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50864"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFF70_04575"
     CDS             complement(<1..225)
                     /locus_tag="VFF70_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44006.1"
                     /translation="MKTKWLLATLFIVFSMGVTACATLAAPPASTAASVQIVQGPPPT
                     PDYSKVTPPPYDKHKTFDAALPPASSGTLKT"
     gene            complement(386..793)
                     /locus_tag="VFF70_04580"
     CDS             complement(386..793)
                     /locus_tag="VFF70_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44007.1"
                     /translation="MTRLSVWPVRTALLYLGAGFLIGALMLFEKGARIDPSWLRLLPL
                     HVEFVLIGWAMQLAMGVAFWILPRFGRGAAHGNQTLGWMAYGLLNIGALSVGIGLWLN
                     VPPIVPLIGRLAELLAAISFMLHAWPRIKAPGA"
     gene            complement(795..1214)
                     /locus_tag="VFF70_04585"
     CDS             complement(795..1214)
                     /locus_tag="VFF70_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44008.1"
                     /translation="MPPLTRTFIKTALIYFVAALLIGVLLLARSAIDLPVEIASLSPI
                     YFHLLMVGWVTQLIFGMLFWMLPKYSKDKPRGNERLVWAAYIFINAGLILRVIGEPIV
                     AIKPEWGLGWLLMLSALLQLIGGWSFLVNAWSRVKEH"
     gene            complement(1290..1676)
                     /locus_tag="VFF70_04590"
     CDS             complement(1290..1676)
                     /locus_tag="VFF70_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY44009.1"
                     /translation="METRWKVFQASQVAHYDQLPLTNVPLFAEEDFSGCLLGFLPGQE
                     LPVHHHDHEHEVFDVLEGSGTMFLDGESIEVKAGSVIFVPAGVHHGFKNTSDSRWLIR
                     ATIHQRVYARHAIKRAINKRLGRARK"
     gene            complement(1744..2823)
                     /locus_tag="VFF70_04595"
     CDS             complement(1744..2823)
                     /locus_tag="VFF70_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44010.1"
                     /translation="MLKLRVRIHLPMMFLVVIVLLTGLWAALIRVGWQLPNIASVAGY
                     HGALMISGFLGTLVSLERATVLQRKWAYAGSLLSSLGALTLLIGLPSEIGRAALVISA
                     AILCVIFVLFLRIRSTIDILTMSLGAVMWLIGNVLWLFGQPIASAVPWWVGFLILTIA
                     GERLELSRVLMHRPLSKTIFRISIGVFTIGLIISLLNFDLGLRVSGVGLILLGLWLIR
                     FDVARFTLRQTGLTRYIAACLLPGYVWLAIGGGLWIIFGGTSIAGFHYDAMLHTLFLG
                     FVFSMIFGHAPIIIPSVMPVDFTYRSIFYLHLVLLQLSLIVRIAADLFAAQPVRMWAG
                     LFNVVAVLLFLGMMLRSVHRRPITA"
BASE COUNT          795 a          856 c          730 g          542 t
ORIGIN      
        1 cgtcttgagc gtgccactcg acgcgggcgg cagcgccgca tcaaaagttt tgtgcttatc
       61 atacggcggc ggtgtaactt tcgaataatc gggcgtgggc ggcgggcctt gcacgatctg
      121 cacggacgcg gccgtcgacg ctggcggtgc ggccagcgtc gcgcacgccg tcacccccat
      181 tgaaaacaca atgaacaaag ttgctaacag ccatttggtt ttcatgtctc tccttgtctg
      241 ctctttttga tagatacacc ataccttagc acggctttaa ctagaaaagt atgattttaa
      301 tcatacggcg atgatgcagt tgattgagtc aaccacaaat cttccgaagc tgaagatcga
      361 cgagcgcatt atcgttcagg cggcatcatg ctccgggcgc ttttatgcgc ggccaggcat
      421 gcagcatgaa actgattgct gccaacaatt ctgccaatcg gccgatcagc ggcacgatcg
      481 gcggcacatt caaccacagt ccgatcccaa cgctcaaggc accaatgttc aacaacccat
      541 aagccatcca gcccaacgtt tgattgccat gcgctgcgcc gcgaccaaag cgcggcaaga
      601 tccagaaagc cacgcccatc gccaactgca ttgcccaacc gatcaacaca aactcaacgt
      661 gcaggggcag caggcgcagc catgacggat cgatgcgcgc gcccttctca aacagcatca
      721 gcgcgccgat cagaaatcca gcaccgagat aaagcagcgc ggtgcgaacc ggccacacac
      781 tcaagcgtgt cacgtcaatg ctcctttacg cgcgaccacg cgttcaccaa aaaagaccag
      841 ccaccgatca attgcagcag cgctgacaac atcaacagcc aacccaaacc ccattcgggt
      901 ttaatcgcta cgatcggttc accgatcact cgcaaaatca aacccgcgtt gatgaaaata
      961 tacgcggccc aaaccaagcg ttcattcccg cgtggcttat ccttcgaata tttgggcagc
     1021 atccagaaca acatgccgaa gatcagctgc gtcacccagc ccaccatcag cagatggaaa
     1081 tagatcggtg acagcgaagc gatctccacc ggcaaatcga tcgccgatcg agcgagcagc
     1141 agcaccccga tcagcagtgc ggccacaaaa taaatcaagg cagttttgat gaaggtacga
     1201 gtaaggggtg gcatggttac gcttgatcct cccgcaggtt tatttagctg gtatggtaat
     1261 ttgggcgaaa cctgcgggag gattcctatt tactttcgag cgcggcccaa gcgtttattg
     1321 atcgcgcgct tgattgcgtg ccgtgcatac acacgctgat ggatcgtcgc gcgtatcagc
     1381 cagcggctgt cgctggtatt cttgaagccg tgatggactc ccgccggaac aaaaatcacg
     1441 ctgccggcct tgacctcgat cgattcgccg tcaaggaaca tcgtcccgct cccttcgagc
     1501 acatcaaaaa cttcatgctc gtgatcgtga tgatgaacag gcaattcttg tcccggcaaa
     1561 aaaccgagca ggcaaccaga gaaatcttct tcggcaaata gcggcacatt ggttaatggc
     1621 aattgatcgt agtgtgcaac ttgcgaggct tgaaaaactt tccagcgagt ctccatccca
     1681 tctcccctgt catggtatag acgctccggt ggcacgtcga taagacaatt tgccggatcg
     1741 tctctacgca gtgatcggtc tgcgatgcac cgatcgaagc atcattccca agaataacag
     1801 cacagccact acattaaaca acccggccca catccgaacg ggttgggcgg caaagagatc
     1861 ggcggcgatg cggacaatca atgacagttg caataacaca agatgaagat aaaagatcga
     1921 tcgataggtg aagtctaccg gcatgacgga cggaataata atcggcgcgt ggccgaagat
     1981 catcgagaag acgaagccga ggaacagcgt gtgcagcatc gcgtcatagt gaaatccggc
     2041 gatcgatgtg ccgccgaaga tgatccacaa cccgccaccg atcgccaacc acacgtaacc
     2101 gggcagtaga cacgctgcga tatagcgcgt caagcccgtc tgccgaagcg tgaagcgcgc
     2161 cacatcgaag cggatcagcc acaatcctaa caggatcaag cctacgccgc tcacgcgcag
     2221 acccagatcg aaattcaaca gagaaataat caacccgatt gtgaacacgc cgatcgagat
     2281 tctgaagatc gttttactga gcgggcggtg catcaacacg cgcgatagtt ccaagcgctc
     2341 accggcgatc gtcaggatca aaaatccaac ccaccacggg accgcagacg caatcggttg
     2401 gccgaacaac cacagcacat tgccgatcag ccacatgacc gcgcccaggc tcatcgtcag
     2461 gatatcgatc gtcgatcgaa tgcgaagaaa caagacgaag atcacgcaca agatcgcagc
     2521 acttatcacc aaggccgcgc gcccgatctc cgaaggcaaa ccgatcaaca aggtcaatgc
     2581 gccaagactg gacaacaacg acccggcata agcccacttg cgctgcaaca cggtggcacg
     2641 ttcgaggctg accagcgtgc caagaaagcc cgagatcatc agcgcgccgt gataaccagc
     2701 cacggatgcg atgttgggca attgccagcc aacgcggatc aacgccgccc ataatccggt
     2761 cagcaggacg atgaccacca agaacatcat gggcagatga atacgcacgc gcagttttaa
     2821 catgaagtta tctctggttg atcatagtct cgatcgtcaa gaacgtaaat gatttagatc
     2881 atccaacaaa aaggccgaag gttgtggcct tcggcctttc atg
//
LOCUS       DASRTJ010000218         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_239962,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000218 DASRTJ010000000
VERSION     DASRTJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239962"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFF70_04600"
     CDS             <1..346
                     /locus_tag="VFF70_04600"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HZY44011.1"
                     /translation="NDDYGRAAKQAVERGESIEQIPTIPDRPNIFILYEQNIGTLTPM
                     IVEELRAAEQEFSPQLIAEAFQEAANQNVRSWAYIRKILDNRSRKGKSNETRGRDVAA
                     DWQRILQKDKRK"
     gene            348..1673
                     /locus_tag="VFF70_04605"
     CDS             348..1673
                     /locus_tag="VFF70_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44012.1"
                     /translation="MASANDVCKICGGLGWISAEVPVGHALFGKLVPCPHRAAERDQS
                     VAVKVWSDLGPLRSMTFENFQPDGHAATIEQRTSLQTAFDRSKKFAEEPREWLLLQGS
                     YGCGKTHLAAAIANACLERGVPVRFVNVPDLLDYLRGAYNPSVEETYDARFSEVRDAP
                     ILILDDLGTQNATPWAEEKLYQILNTRYMAKLPTVITTNLDLDDLDPRLRSRLSDLDL
                     VRKLPVLAPDFRRSAIEQGQSSMSSLASYHDKTFETFDLRAGNLSREEVSNLKDALRL
                     AKQFAQHPHDWLMFTGSYGAGKTHLAAAIANYQAERGYPAMFVVVPDLLDHLRATFSP
                     EARTSFDQRFEEVRAAPLLILDDLGTESATAWAREKLYQIINHRHVSRLPTVITTSVS
                     PDDLDPRIRTRILDETRCTVFPLIVPSFRGGPAARPIAKQTRSTSSRRR"
     gene            1712..3898
                     /locus_tag="VFF70_04610"
     CDS             1712..3898
                     /locus_tag="VFF70_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZY44013.1"
                     /translation="MPLVTISIGWLTGIWIASMVALPVEAILIAALVPIIGMVLWWHE
                     TRWRMIWLATLFSIFGAIRYQLAIPHFDQNSLTTYNNIDQVTLEGVIDTEPDVRDTYL
                     NLRVNADQLTLPDGTTRSINGLVLVRPSRPAEYKYGDRIRVTGDLIAPPEFATFSYKD
                     FLARQNIYSMIDRPRVGLIAHDQASPILGAILAFKDSARNTIRKILVEPEASLLNGIL
                     LGDDAALPQNVQADFRNTGTTHIIAISGYNITILISIISAVTVRLFGRRRSFWVLIIA
                     LIVYTIMVGASASVVRASIMGIGTLFAIYLGRQGSALNWLFAAGLIMTLLDPNTLFDV
                     GFQLSFAATLGLIVYARSFANATQRLITRMLSNRIAKMIVEVINDALLVTLAAQVASL
                     PLLMYYFQQFSWVSFLVNPLVLPAQSGVMVFGLIALAAGLIFIPIGSIIAWSAWIFLA
                     WTINVIHLFALIQGQPVALGYVSPIVPVIYYAILIFITWKVRSRASLQIKWLTPRRLI
                     FAGGLVVVLIGVALSWQPDGQLHIVALDVDGHPILVRTPQGKQILIGGSSSPSGLLSA
                     IGQQLPFWDRDIDLLIVPHASVAELNGLLAVVDRYSIGQVVSVDVPLNSQAGRNWQLA
                     LSKLHLTSVPISTTSTIDLEPDVSIEFDRSIVLIAARDHVIGLGPSDAAQIDVISDNI
                     AALPEQPQMIFVWQSNLSDARVVDLTDRGTLDLSLSPAGVTIGTLK"
BASE COUNT          818 a         1063 c         1062 g          956 t
ORIGIN      
        1 gaatgatgat tatggccgcg cggcgaagca agccgttgag cgtggtgaat cgatcgagca
       61 gattccaacg atcccagatc gaccgaacat tttcatattg tacgagcaga acatcggcac
      121 gttgacgccg atgatcgtcg aagaattacg cgctgcggag caagaatttt caccgcagtt
      181 gatcgccgag gcatttcaag aagcagccaa tcaaaacgtt cgatcctggg cttatattcg
      241 caagattcta gacaaccgat cgcgcaaagg taaatcgaat gaaacgcgtg ggcgagatgt
      301 tgccgccgat tggcaacgca tcctccaaaa agacaaacgc aaataacgtg gcttcagcta
      361 acgacgtatg caaaatttgc ggcggccttg gctggatcag tgccgaggtg ccggttggac
      421 atgcgctgtt cggcaagctc gtgccgtgtc cgcaccgtgc ggccgagcgg gatcaatcgg
      481 tcgccgtaaa agtctggagc gatctcggcc cgctacgcag catgaccttc gaaaattttc
      541 agccggacgg gcacgcggcg acgatcgaac aacgcacttc attgcagact gcttttgatc
      601 gatcgaaaaa atttgccgaa gagccgcgtg agtggctgtt gttgcagggc agctatggct
      661 gcggcaagac gcatttggcc gcggcgattg cgaatgcctg tttggagcgc ggtgtgccgg
      721 tgcgctttgt gaatgtgccg gatttgttgg attatttgcg cggcgcgtac aatccgtcgg
      781 tcgaagaaac ttacgacgcg cgcttcagtg aagtgcgtga tgcgccgatc ttgatcctgg
      841 acgatctcgg cacgcagaac gccacgccgt gggctgaaga gaagctgtat caaatcctca
      901 atacgcgcta tatggccaaa cttccgacag tgatcacaac caacctcgat ctggacgatc
      961 ttgatccgcg cttgcgatcg cgtttatccg atctcgatct ggtgcgcaag ctgcctgtcc
     1021 tcgcgcccga ttttcggcgc agcgccatcg agcagggcca aagctcgatg agcagcctgg
     1081 cgtcgtatca cgacaaaact ttcgagacgt tcgatctccg tgcaggcaat ctgtcacgcg
     1141 aagaagtctc aaatctcaaa gacgccctgc gcttggccaa acaattcgcg cagcacccgc
     1201 acgattggtt gatgttcacg ggcagctatg gcgcgggtaa aactcatctg gccgcggcga
     1261 ttgcgaacta tcaagccgag cgtggttatc cggcgatgtt cgtcgtggtg ccggatctgc
     1321 tcgatcatct acgcgccact ttcagcccgg aagcacgtac gtcattcgat cagcgcttcg
     1381 aagaagtgcg tgccgcgccc ttattgattc tggacgatct cggcactgaa tcggctacgg
     1441 cttgggcgcg cgagaaattg tatcaaatca taaaccatcg gcacgtcagt cgattgccga
     1501 cggtgatcac cacctccgtt tcaccggacg atcttgatcc acgcattcgc acgcgcatcc
     1561 tcgatgaaac gcgctgcacg gtcttcccgc tgatcgtccc aagttttcgc ggcgggccag
     1621 ctgccaggcc gatcgccaag caaacgcgat cgacttcttc acgccgaaga taatttttgc
     1681 tcctcgtctg attgtcacgt ataatcgccg catgccgctg gtcaccatct cgattggctg
     1741 gttaactgga atttggatcg cttcgatggt ggccttaccc gtcgaagcga tcttgatcgc
     1801 cgcgctggtg ccgatcattg gtatggtctt gtggtggcat gaaactcgct ggcgaatgat
     1861 ctggcttgca actctatttt ccatctttgg cgcgattcgt tatcagctcg ccattcctca
     1921 cttcgatcaa aactcgctta caacttacaa caacatcgat caagtcacgc ttgaaggcgt
     1981 gatcgatacc gaaccggatg tgcgcgatac atacctcaac ctgcgcgtga acgccgatca
     2041 gttgacgctg cccgatggca caactcgatc gatcaacggc ttggtcttgg ttcgaccgag
     2101 ccgcccggcc gaatacaaat atggcgatcg cattcgcgtc acaggcgatc taatcgcgcc
     2161 gcccgaattt gccacattct cttataaaga tttcctcgcg cggcaaaata tttactcgat
     2221 gatcgatcga ccgcgggtcg gcctcatcgc gcacgatcaa gccagcccca tcctcggcgc
     2281 gatcctcgcc ttcaaagata gtgcgcggaa tacgatccgg aaaattttag ttgaaccgga
     2341 ggcctcgctc ttgaacggca tcttgctggg cgatgatgct gccttgccgc aaaatgtgca
     2401 agcagacttc cgcaacaccg gcacgacgca cattattgcg atcagtggct ataacatcac
     2461 gattctgatt tcgatcattt cggcggtgac cgtgcgcttg tttggccgcc gtcgatcgtt
     2521 ttgggtgctc atcatcgctt tgatcgtcta cacgatcatg gttggtgcgt cagcttcagt
     2581 ggtgcgcgca tcgatcatgg gcatcggtac actttttgcg atttatctcg gtcgacaagg
     2641 cagtgctttg aattggttgt tcgccgcggg cttgatcatg acgctgctcg atccgaacac
     2701 gttgttcgat gtcggctttc aattgagttt cgccgcgacg ttgggtttga ttgtatatgc
     2761 ccgatcgttt gcgaatgcga ctcaaagatt gatcacgcgg atgctctcga atcgcatcgc
     2821 taagatgatc gtcgaagtga tcaatgatgc gctgctcgtt accttagccg cacaagtcgc
     2881 ctcgctgcca ctgctgatgt attacttcca gcaattctcg tgggtatcgt tcctcgtgaa
     2941 cccgttggtc ttaccggctc aatcgggtgt gatggtcttt ggcttgatcg cgctggccgc
     3001 cggcttgatc ttcattccga tcggatcgat tatcgcgtgg tcggcgtgga tcttcttggc
     3061 atggacgatc aatgtaattc atttattcgc actgattcaa ggtcaaccgg tggcgttggg
     3121 ttacgtcagc ccgatcgtgc cggtgatcta ttatgcgatc ttgatcttta ttacatggaa
     3181 ggtgcgcagt cgcgcgtcac tacaaataaa atggctgaca ccgcgccgct tgatctttgc
     3241 gggcggcctg gtcgtggtct tgatcggcgt cgcgttgtcg tggcagccgg acggccagct
     3301 gcacatcgtc gcgctcgatg ttgacggtca tccgatcttg gtgcgcactc ctcaaggcaa
     3361 acaaattctc atcggcggat cgagcagtcc gtccgggctg ctgagtgcga ttggacagca
     3421 gctgcccttt tgggatcgag acatcgatct attgatcgtt ccgcacgcct ctgtcgctga
     3481 actaaatggt ttgctggctg tagtcgatcg atattcgatc ggccaagttg tttcggtcga
     3541 cgtgccgttg aatagccaag cgggccgcaa ctggcaattg gctttaagca aactccattt
     3601 gacttcagtt ccgatcagca ccacctcgac gatcgatctt gaacccgacg tctcgatcga
     3661 gtttgataga tcgatcgtgt tgattgcagc gcgcgatcat gtcattggcc tgggacccag
     3721 tgacgctgcg cagatcgatg tgattagtga taacattgcg gcgctgcccg aacagccgca
     3781 gatgatcttc gtctggcaat ccaatctatc agatgctcgc gtggtcgatc taacggatcg
     3841 cggcacactt gacttgagtt taagtccagc gggtgtaacg atcggcactt tgaaatagt
//
LOCUS       DASRTJ010000219         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_346097,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000219 DASRTJ010000000
VERSION     DASRTJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346097"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFF70_04615"
     CDS             complement(<1..610)
                     /locus_tag="VFF70_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZY44014.1"
                     /translation="MTRIGNWIEERSGWRTVWQTIFKRKIPKVNWFYALGSATLFVAI
                     NQIVTGILLTIYYVPTPDHAYDSVQYITSQVPAGWLIRGLHHWGASAMVVLVVLHMLR
                     VILNGSYKFPREVTWLTGVVLLLVTVGFGFTGYLLPWDQKAYWATTVGTRIAGSAPLI
                     GDSLLRIMRGGPDLSAVTLARFFGVHVWVMPAALLTFVFIHLY"
     gene            complement(607..1092)
                     /locus_tag="VFF70_04620"
     CDS             complement(607..1092)
                     /locus_tag="VFF70_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZY44015.1"
                     /translation="MDEEKQITRREFSSIAIWAIGGLITVGIGLPAIAYIVGPALERS
                     ATDDWIHVGAASKVELGAPTLFKVKIQLKSGWIVNEEELSIYVRTDDGRTFTAMSNVC
                     THLGCRVRWAADRKQFFCPCHNGVFDQEGNVLSGPPPKPLNRYEVKVEDGQLSIRGGQ
                     S"
     gene            complement(1204..>2764)
                     /locus_tag="VFF70_04625"
     CDS             complement(1204..>2764)
                     /locus_tag="VFF70_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HZY44016.1"
                     /translation="DYTNSSAFCGTTCHTMPPEYAAYLTSPHARILCVECHIGRDFIG
                     SRVLRKAGDLKHVVAMTSGSYTYPLYADAMRPARETCEQCHSPAKFSNDTLVEFKRYA
                     NDINNTATSIFLTLKTGGGNSRQGLGKGIHWHVESQVYYSPTDSLEQTIPYVKVINPD
                     GSLKEYVDATSNIDPKSIDPKNLKEMDCIACHNRITHMILPPADMVDQLMARNVIAAD
                     IPEIHLKATEVLTNYPTVDVGLAAIAGLKNYYQRVHTDYYALNGPLVDAAVTAVQDAF
                     QKSFFPDQKENWQTHASNIGHKDSPGCFRCHDGKHLDANQQSIRLECNLCHSIPVVVG
                     PDRFVADIEISRGAEPQSHLNPNWIGQHPAAFNSQVCSKCHSTSNAGGTDNTSFCSNS
                     ACHANNWQYAGLNAPGLRDVFKAQLPPQPTPVPLPVNGPLTYNATIGPIFRSYCSDCH
                     SDQGMKDLNLTTYQTAMAGSQNGKVIVPGDPTTSLLVKIQSGPKSHFGQLPADLLDLV
                     IKWISAGAPEK"
BASE COUNT          643 a          739 c          766 g          616 t
ORIGIN      
        1 gatacaaatg gatgaagaca aaagtgagca aagcagccgg catgacccaa acgtgcacgc
       61 cgaagaagcg ggccagtgta accgccgata gatctggccc accccgcatg atccgcagaa
      121 gactgtcacc gataagcggc gcgctgccag cgatgcgcgt gccgacggtg gtagcccaat
      181 aggccttttg atcccaaggc agcaagtagc cagtgaaccc gaaaccaacc gtcaccaaca
      241 acaaaaccac accagtcagc cacgttactt cgcgcggaaa tttatacgag ccgttgagga
      301 tcacgcgcag catgtgcaac acgaccaaca ccaccatcgc actcgcgccc cagtggtgca
      361 agcctcgaat caaccaaccg gccggtacct gcgacgtaat gtactgaaca ctgtcatagg
      421 catgatcggg cgtcggcacg tagtagatcg ttaacaagat gcccgtgaca atttgattga
      481 tcgccacgaa caaggtggcg ctgccgaggg cgtaaaacca gttgactttc ggaatcttgc
      541 gtttgaagat ggtctgccag acagtgcgcc aacccgaacg ctcttcaatc cagttgccga
      601 ttctcgtcat gactgacctc ctcgaatgga tagttggccg tcttcaactt ttacctcgta
      661 gcgattgagc ggttttgggg ggggacccga taggacgtta ccctcttgat cgaaaactcc
      721 gttatggcat gggcagaaga attgttttcg atccgcagcc cagcgaacac ggcagcccag
      781 atgggtgcag acgttggaca tggctgtgaa tgtgcggccg tcatcggtcc ggacatagat
      841 cgacagttct tcttcgttaa cgatccagcc ggacttaagc tgaatcttga ccttgaataa
      901 cgtgggggcg ccaagttcta ctttggaggc cgcgcccacg tgaatccaat cgtcggtcgc
      961 gctgcgctcc aatgccgggc cgacgatata ggctatggcc ggtagtccga tgccgacggt
     1021 gatcaaaccg ccgatcgccc aaatcgcaat actcgaaaat tcccgacggg ttatctgctt
     1081 ctcttcatcc atgcctaagc ctccccattt attttactcc ataaaaaata cgaagcccaa
     1141 tgagatcaga gataccgatc gatgatcgaa gagccgatcg aatccctgaa tcaactcagc
     1201 cctttacttc tcgggcgcgc ccgctgaaat ccacttgata accaaatcca atagatcggc
     1261 gggcagttga ccaaagtgtg atttcggtcc gctttggatc ttcaccagta aactagtagt
     1321 tggatcgccc ggcacaatca ctttgccatt ttgactgccg gccatagccg tttgataagt
     1381 cgtcagattc aagtccttca tgccctggtc gctatggcaa tcactgcaat acgatcggaa
     1441 gatagggcca atcgtagcgt tgtaggtcaa cgggccattc acgggcagtg gaaccggggt
     1501 cggctgaggc ggcaactgcg ccttgaagac atcgcgcaga ccgggcgcat tcaagcccgc
     1561 atactgccag ttgttagcat ggcacgcgct gttcgagcag aacgaagtgt tatccgtgcc
     1621 gccggcattt gaagttgaat ggcatttgga acatacttgg ctattgaacg cagcgggatg
     1681 ttggccgatc cagttcggat tcagatgcga ttgcggttcc gcgccccggc tgatctcaat
     1741 gtcggccaca aaccgatcgg ggccaacgac gaccggaatg gagtggcaca agttgcattc
     1801 caatcgaatc gactgctgat ttgcatcgag atgtttgcca tcgtgacaac ggaagcagcc
     1861 gggcgagtcc ttgtgcccga tgttgctggc gtgcgtctgc caattttctt tctgatccgg
     1921 gaagaaactc ttttggaaag catcttgcac tgccgtcact gcggcatcga ccagcggccc
     1981 gttgagcgca tagtagtctg tatggacgcg ctggtaataa tttttcagcc cggcgatggc
     2041 tgctaagcca acgtcaaccg tgggatagtt cgtcaagact tctgttgctt tgagatggat
     2101 ctccggaata tccgccgcga tcacattgcg cgccatcaat tgatcgacca tatcggccgg
     2161 cgggaggatc atgtgcgtga tgcggttgtg gcaggcgatg cagtccatct ctttgaggtt
     2221 cttgggatcg atcgatttgg gatcgatatt cgaggtggca tcgacatatt ccttgaggct
     2281 gccgtcggga ttgatgactt tcacatatgg aatggtctgt tcaagagaat cggtcggcga
     2341 gtaatacact tggctctcaa catgccagtg gatgcccttg cctaaacctt ggcgggaatt
     2401 gccgccgccg gtcttcagtg tgaggaaaat actggtggct gtattgttga tgtcgttggc
     2461 gtaacgcttg aactccacca gcgtatcgtt ggagaattta gcgggcgagt ggcactgctc
     2521 gcaggtttcg cgggcgggcc gcatcgcatc cgcatacaac ggataggtat acgaaccgga
     2581 ggtcatggca acaacgtgct taagatcgcc cgctttgcgc aggacccggc tgccgatgaa
     2641 gtcgcgcccg atatgacact caacacagag aattcgcgca tgcggcgaag ttaaataggc
     2701 ggcgtattcg ggcggcatgg tgtggcaggt cgtcccgcaa aaagctgatg agttggtata
     2761 gtcc
//
LOCUS       DASRTJ010000220         9580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_315996,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000220 DASRTJ010000000
VERSION     DASRTJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315996"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFF70_04630"
     CDS             <1..368
                     /locus_tag="VFF70_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZY44017.1"
                     /translation="TVIGPTANFKGTLQSDGGLRIDGIVEGTVLVAGNLIIGESGKVI
                     ADITAQNVSVAGAIKGTVKAAGRLEILSSGRVWGDISVASFLIDEGGFFRGQSIMPGE
                     PDMPMLEAPMGDGQVVESN"
     gene            511..660
                     /locus_tag="VFF70_04635"
     CDS             511..660
                     /locus_tag="VFF70_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HZY44018.1"
                     /translation="MPRISEFFDVVIYMYFNDHSPSHFHAEYGEFEAVYAIDTLDILR
                     GHLPR"
     gene            785..1069
                     /locus_tag="VFF70_04640"
     CDS             785..1069
                     /locus_tag="VFF70_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZY44019.1"
                     /translation="MEQLGPRVRVRAVEPLRGFTVRIHFDNNTEREMDLAPYLRGPIF
                     EPIRNDPAKFRSIKIVGGTLAWDNGADIDPDVLYYNLKPAWMENEEAVQA"
     gene            1195..1668
                     /locus_tag="VFF70_04645"
     CDS             1195..1668
                     /locus_tag="VFF70_04645"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZY44020.1"
                     /translation="MLKAHLDLLRRAIDVARQARAHGNHPFGAILVDAHGSILLEAEN
                     SVVTDRDCTGHAELNLMRKASQQFDRSVLAECTLYTSTEPCAMCAGSIFWGGVSRLVY
                     GLSEVGLLEIVGDSAPDSFLSLPCREVFAHGLRSIEVIGPLLEDEARQVHAGFWR"
     gene            complement(1733..3280)
                     /locus_tag="VFF70_04650"
     CDS             complement(1733..3280)
                     /locus_tag="VFF70_04650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44021.1"
                     /translation="MAWSVLGVADQEIGAAVQRLLQRSLGFVMLIVIPFTIATTWLAL
                     YGLHVLSLVVLYLRHRHDRLQPIALTDYPIVTVQLPMYNEPHVVERVIEAAANLDWPH
                     DRLQIQVLDDSTDRTTALARSRIEAQRARGLDIELIHRSNRAAFKAGALSAGLDQATG
                     SFIAIFDADFVPSPNFLKLMLPHFTDNSIGFVQARWDHLSTSAPLAQALAIGVDGHFT
                     VEQFSRNRSNLPMIFNGSAGIWRRECIESSGGWGQDTLCEDMDLSLRAAMQGWRCVFV
                     PDVTVLQEEPDQVVSIKTQHGRWAKGGAQCLRKLSVPLMKSKLTLRQKFFSLMYLSGY
                     AAHLMMLIVILTWLPLSLHPQWLQQMPLTFLGLGGIGLPLEHVLSQMVLYRDWRRKLI
                     MFPLLLAIGFGLTFNNALNVVEGFLNRSIEFKRTPKRGSSAQSSIGIDPKQTRQAWIE
                     VALSIYTLISAGFMIQAGQIITALILVLYSVGFALIGGGTLQSVLSLTRVRADRSTRS
                     VSDEVLG"
     gene            complement(3356..5101)
                     /locus_tag="VFF70_04655"
     CDS             complement(3356..5101)
                     /locus_tag="VFF70_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZY44022.1"
                     /translation="MLLVRVPMAHGDLKALLADLKTQLQEQASSLTRLSGSPDVAKAQ
                     QALLRIQSMMPRFETAVEQLVREREALQALYEVGQAVNSSLDLSAVLNRVMDQIIQLT
                     GAERSFLMLKNETSGELEVRAARNVDKESLDQTEFNVSRTIVQQVVQDMKPIVTTNAQ
                     ADPRFASQESVVAYSLRSILCVPMLVRDQVTGVIYADNRIKTGLFNDKDRDLLAALAN
                     QAALAIENARLYERVKQQLDAITILKDLMDNVFASITSGVITTDMDDRVTLFNHAAES
                     ILGVQAHDTIDQQYNATLPVASDLAPGIDDVKQNHKVASREIERDLPMRGKVELSLNF
                     APLKGSGTGDHSMSGVAIVVDDLTEKRRLEKDQRFIKDTFMRYAPPAVVEAILRDPSQ
                     LKLGGARKFISIIFADIRGFTTFSEKLAPEPLVEILNQYLGIAADAVLAFEGTLDKFM
                     GDAVMGLFNVPLDQADHTLCAVKAALKMDQDIQAYHQKVAIDAQLNYGVGINVGDAIV
                     GNIGTDKQRNYTAIGDSVNYAKRLQENAKGGQILLSQAAYQLVNAQVVVEELPPLQVK
                     GRSVSEPVFQVLGLK"
     gene            complement(5250..7628)
                     /locus_tag="VFF70_04660"
     CDS             complement(5250..7628)
                     /locus_tag="VFF70_04660"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HZY44023.1"
                     /translation="MKRNLLESPYPIDRLALILVGAAVFSAAWSVASAEWMPRLDLLG
                     NVVVIGLLLSAVIATRRWRTRTAHIVMLLYGVAMVAWITIDQMPDKIYGWTWLDTLRL
                     MIVRLGEHIWLWFNSIASGGVGKDNSIFLMFLAAIFWLISFSAAWNTFRYKQLWRAVA
                     PAGVILLVNTFYYGGDRPLTSLVIVYIFCVLLYAARMYTLSQQQRWASGRVGHSSEIQ
                     RDFLRIGSGIALAAVLFGAVAPTVFGAPQISDLWHEISRPLRSVEDSFSRMFSGLEPH
                     GIPYANPFGRTLALLGARTLGTEVVMVVRANAGRYWQAVVYDEYTSNGWQSSESQRLT
                     LNPTDPPLTANYDQRVAISQTFTLHFPNNTLIFAAPEPIAVNRPVWVETLPSTSNVDA
                     TIWTSIQPLNDGDSYRVVSSLSTATTDQLRAAGTVYPRSISDRYLQLPNSLPSRVRDL
                     ARQIVTKANAANSFDQAAALEAWLRTNIVYNDAIPGPQAGQDGVDYVLFDAKKGYCDY
                     YASAMAVMARSLGIPARVATGYASGEYDRRTGLYQVHQYDAHTWVEVYFPQYGWTEFE
                     PTASQPTIIRNLSGSGAAGLANPRGDPSGVEGGLSTFRNRNIPEGGQFDFPDVPANGQ
                     TTPVDPAAAAADQTPWSTLIISAVVLLLAGSLIAMWLFENKDRTQRSRAGEWAFARML
                     RMSQWLRVSVSPAQTPFEQADQLKAAMPSSAGRIDRVAGLFVREQYGRSVVDRSEARS
                     IWSQLHWKMWWSGLVRRLPRSISLPRWLLRRRSIRPSLRGVRSTTKQSPDRI"
     gene            complement(8138..>9580)
                     /locus_tag="VFF70_04665"
     CDS             complement(8138..>9580)
                     /locus_tag="VFF70_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZY44024.1"
                     /translation="LFGVSAPVLANASHAAQAAPASTIVVDAGSDENVCSGFLTLRCA
                     INYANANPGTWIRFAPGLPAVLLQSALPTISGDSTWIDGHDLSNGCVCPRIDASFAGL
                     WTGNNGLTINANYVTISNIKVVNIPSGADISIAGGKDIEISYDYLGILPNATQCGSLS
                     QSTVGVDVVNDRAGSGGSHDNGVAYIYANTISCHGGSGVEIVNSNYVYVGQQRDDLTN
                     HANYIGTTSDGLGAAGNGYAGVRVAVNSDQIAIRYNLIAHNANGGIIVDGTNIDVSFN
                     TLSANYWGLAISSGSTQSIIGNKIGTSANGLLPLPNAHEGILITGGSGLFLSDNLVAY
                     NHAAGIAITGSSTHALLENNDIYRNGGLPIDLGNDGATVNGSHGSVGPNNWLKYPVVT
                     AFSGSLVQGTTCPSCAVYIYKAVGNPAAPGGGGLFRTNVLANSSGEWSVNLPNSLTRL
                     DVTFTTFDGGGNSSEMSPRPQMFLPLISRH"
BASE COUNT         2196 a         2736 c         2661 g         1987 t
ORIGIN      
        1 aaacggtcat cggcccaacg gccaatttca aaggcacgct gcaaagtgac ggcggcctgc
       61 gcatcgacgg catcgtcgaa ggcactgttc tcgtcgccgg aaatttgatc atcggcgaaa
      121 gcggcaaagt gatcgcggac atcaccgcgc agaatgtctc ggtggcgggc gcgatcaaag
      181 gcacagtcaa agccgcgggc cgcttggaga ttctatcgag cgggcgcgta tggggcgaca
      241 tttcggtggc gtcattcttg atcgacgaag gcggcttctt ccgcggccag agtatcatgc
      301 ccggcgaacc ggatatgccg atgctcgagg cgccaatggg cgatgggcaa gtggtcgaaa
      361 gcaattaatc ggttcgatac acagccggtc tcttcaagag gccggttttt gttttccaat
      421 gcgtgttgag tttgctcttg acaatagatg atcgtatact taaaacttca ttcggttaat
      481 tggtgtatca tctagccgga gacaaagctc atgcctagaa tctcagagtt cttcgacgtc
      541 gtgatctata tgtacttcaa tgaccactcc ccgtctcact ttcacgctga gtatggcgag
      601 ttcgaagccg tctatgcgat tgatacattg gacatcttgc gaggtcactt gccgcgatga
      661 gcccacagtt tggtgatcga gtgggcgcta gcccatcgcg ccgaattgag agacgactgg
      721 aacaaagcgc gcgagcaact accgttagac gagattgagc ctttggagta agaggagtac
      781 agccatggaa caattaggtc cgcgtgttcg agttcgcgcc gtagaaccgc ttcggggatt
      841 cacggtgcgc attcacttcg acaataatac ggaaagggaa atggatttgg cgccatacct
      901 gcgcggccca atcttcgagc cgatccgaaa cgacccggct aagtttcgtt cgatcaagat
      961 tgtgggtggt actctcgcgt gggacaacgg cgcagatatc gatccggatg tcctctatta
     1021 caatttgaaa ccggcgtgga tggagaatga agaagcagtt caagcataag gccgatctcg
     1081 ctggaagcgc tcgtcggcaa cggccagtag tcgacaagaa ttactctgct caatataaag
     1141 ccggtttcgt gaagaggccg gtttttattt atctcgccgt ataggaaggc aatcatgctt
     1201 aaagcccatc tcgatctttt acgccgcgcg atcgatgttg cccgacaagc ccgcgcgcat
     1261 ggcaatcatc cgttcggggc gatcctggtc gatgcgcatg gatcgatctt gctcgaagcc
     1321 gagaacagcg tcgtaaccga tcgggattgc accgggcacg ccgagttgaa cttgatgcga
     1381 aaggcttcgc aacaattcga tcgatcggtg ctggctgaat gcacgttgta caccagcacc
     1441 gaaccgtgcg cgatgtgcgc cggatcgatc ttctggggcg gtgtcagccg cttagtgtac
     1501 ggactcagcg aagtgggctt gctggaaatt gtcggcgaca gtgcgcccga ttcgtttttg
     1561 tcgctgccct gccgcgaagt gttcgcgcac ggcctgcgat cgatcgaggt gattgggccg
     1621 ctgctcgaag acgaagcccg gcaagtgcat gctggttttt ggagatagag gcttcagcaa
     1681 acattatgca agtgctctaa gcccgcgtcc acgcgggcgt ttcactaaag ttttacccca
     1741 gcacttcatc gctcacactg cgcgttgatc tatccgcgcg gacgcgagtg agggacaata
     1801 cagattgcaa cgtcccgccg ccgatcaacg cgaagccgac tgagtagagc accaggatca
     1861 aagccgtgat aatttgtccc gcttgaatca tgaagcccgc ggagatcaaa gtgtaaattg
     1921 atagcgccac ttcgatccag gcttgccgcg tttgcttcgg atcgatcccg atcgaactct
     1981 gagcactcga tccacgtttg ggcgtgcgct tgaactcgat cgatcgattc aagaagcctt
     2041 cgacgacatt cagcgcattg ttgaacgtca ggccgaaacc gatcgccagc aagagcggga
     2101 acatgatcag cttacgccgc caatcgcgat acagcaccat ttgcgacagc acatgctcca
     2161 agggcaaacc gatgccgcca aggccgagaa acgtcaacgg catttgctgc agccactgcg
     2221 gatgcagcga cagcggcagc caggtcagaa tcacgatcag catcatcaaa tgcgcggcgt
     2281 aaccgctaag atacatcagg ctgaaaaatt tttgccgcag cgttaatttt gatttcatta
     2341 acggcacgct gagttttctc aaacactgcg caccgccctt cgcccagcgg ccatgttgag
     2401 tcttgatgct gaccacctga tccggttctt cttgcaagac cgtcacgtcc ggcacgaaca
     2461 cgcagcgcca gccctgcatc gccgcgcgca aactcaggtc catatcttcg cacaacgtat
     2521 cttgtcccca gccgccgctc gattcgatgc actctctgcg ccagatgccc gctgatccgt
     2581 tgaagatcat cggcaggttc gatcgattgc gggagaattg ctcgacggtg aaatgtccgt
     2641 ccacgccaat cgctaaggcc tgtgcgagcg gtgcactggt cgataaatga tcccaacggg
     2701 cttgcacaaa cccgatcgag ttgtcggtga aatgcggcag catcagtttc aagaaatttg
     2761 gcgagggcac aaagtcggcg tcaaagatcg cgataaagct gcctgtggct tgatcaagtc
     2821 cggcgctcaa cgcgccggct ttaaaagccg cgcggttcga tcgatggatc aactcaatat
     2881 cgagcccgcg cgcgcgttgg gcttcgatcc gcgatcgggc caacgcggtg gtccgatcgg
     2941 tggaatcgtc cagcacctga atctgcaatc gatcgtgcgg ccaatcgaga ttggcggctg
     3001 cctcgatcac tcgttcgacg acatgcggct cgttatacat tggcagctgc acggtgacga
     3061 tcggataatc cgtcagcgcg atcggctgca atcgatcgtg ccggtggcgc agatacaaca
     3121 cgacgaggct caagacatgc aagccataca acgccagcca agtggtagca atggtaaatg
     3181 gaataacaat cagcataaca aatcccaagc tgcgttggag cagccgttga actgctgctc
     3241 caatttcttg atcagcgacg cccagtacag accaggccac aaacaaaacc gcgacagagg
     3301 tcgcggtcag cagtatacat ccacccagtt gagcaaggat gcctcgccct tgcttttact
     3361 tcaaacccag cacttgaaac accggctcag atacactgcg gcctttcact tgcagcggcg
     3421 gcagctcttc gaccacgact tgagcgttga ccaactgata cgcggcctga ctcaacaaaa
     3481 tttgcccgcc tttggcgttc tcctgcaagc gcttggcgta gttcacgctg tcgccgatgg
     3541 cggtatagtt gcgctgcttg tccgtgccga tgttgccgac gatcgcatcg ccgacattaa
     3601 tgccgacacc gtaattgagt tgcgcgtcaa tggccacttt ctggtgatac gcttgaatat
     3661 cctggtccat cttcagcgcc gctttgacgg cgcacagggt gtgatcggct tgatcgagcg
     3721 gcacgttgaa caagcccatg accgcatcgc ccataaattt atcgagcgtg ccttcaaaag
     3781 ccagcaccgc atcggcggcg atgcccaggt actggtttaa aatttcgacc agcggctctg
     3841 gcgcgagttt ctcgctgaag gtcgtgaagc cgcgaatatc ggcaaagatg atcgagatga
     3901 atttgcgcgc gccgcccaac ttcaactgcg acggatcgcg cagaatcgct tcaaccacgg
     3961 ccggcggcgc atagcgcatg aacgtgtctt tgatgaaacg ctggtctttt tccaagcggc
     4021 gcttctcggt caaatcgtcg acaacgatcg ccacgccgga catgctatgg tcgcccgtgc
     4081 cactgccctt gagcggcgcg aagttcaggc tgagctcgac tttgccgcgc atcggcaggt
     4141 cgcgttcgat ctcacgcgaa gctactttgt gattctgctt gacgtcgtcg atgccgggcg
     4201 ccaaatcgct ggcgacgggt aacgtcgcgt tgtattgttg atcaatcgtg tcgtgcgctt
     4261 gcacccccag gatcgattcg gcggcgtgat tgaagagggt cacgcgatcg tccatatcgg
     4321 tcgtgatcac gcctgaggta atggacgcga acacgttatc catgagatct ttcaggatcg
     4381 tgatcgcgtc cagctgctgc ttgacgcgtt catacaggcg cgcgttctca atcgccagtg
     4441 cggcttgatt cgccagcgcc gccaggagat cgcgatcttt atcattgaat agtccggtct
     4501 tgatgcgatt gtccgcgtag atcacgccgg tcacttgatc gcgcaccagc atgggcacac
     4561 acaagatact gcgtaagctg taagccacga cgctttcttg cgaagcgaat cgcggatcgg
     4621 cctgggcatt cgtggtcaca atgggtttca tgtcctgcac gacttgctgc acgatggtac
     4681 ggctgacatt gaattctgtt tggtccagcg actctttatc gacgttgcgc gcggcgcgca
     4741 cttccagttc gccggacgtt tcatttttca acatcaagaa actgcgttcc gcgccggtga
     4801 gttgaataat ttgatccatg acccgattga gcacggcgga caaatcgagc gaggagttga
     4861 cggcctgacc cacttcatac aaggcttgca gggcttcccg ctcacgcacc agctgttcca
     4921 ccgccgtctc aaagcgcggc atcatgcttt gaatcctcag cagggcttgc tgggctttag
     4981 ccacatccgg cgagccggac aagcgggtca gcgacgaggc ttgctcctgc agctgtgtct
     5041 tcagatcagc caacagcgcc ttcagatcgc cgtgcgccat cggcacgcga actaacaaca
     5101 tggaatcgcc tacagacatt gggggacgat cgggcacagc actaccctcg acattcactt
     5161 gaagtgacaa cgggttgaat ctcgaagatt atactcgatc tcattgcaca gtcaaacaaa
     5221 ttggggatcg tcattgccac gcagcaatct caaattcgat caggggattg cttcgtcgtg
     5281 ctacgcactc ctcgcaatga cgggcgtatc gatcggcggc gcagcaacca gcgaggcagc
     5341 gagatcgatc ggggcaagcg gcgcaccaaa ccagaccacc acattttcca atgcagctga
     5401 ctccagatcg atcgcgcttc cgatcgatcc accaccgatc ggccatactg ctcccgcacg
     5461 aataagccgg ccacccgatc gatccggccg gcgcttgacg gcatggcagc cttcaattga
     5521 tcggcttgct caaacggggt ctgcgccgga gagacggaga cccgcagcca ttgcgacatc
     5581 cgcagcatgc gcgcaaaagc ccactcacct gcgcgcgatc gttgggtgcg atctttgttt
     5641 tcaaagagcc acatggcgat caacgatccg gccagcagca acaccacggc actgatgatt
     5701 aacgtcgacc agggagtttg atcggccgcg gcggccgccg gatcgaccgg tgtggtctga
     5761 ccattcgccg gcacgtcagg aaaatcgaat tggccgcctt cgggtatgtt gcggttgcgg
     5821 aaagtggata aaccgccctc aacaccactc gggtctccgc gcggattcgc cagacccgcc
     5881 gctccgctgc cggagaggtt gcggatgatg gtcggttgac tggccgtcgg ctcaaattca
     5941 gtccagccat attgcggaaa ataaacttcg acccaggtgt gcgcatcata ttgatggact
     6001 tgatacaggc cggtccttcg atcgtactcg ccgctggcat aaccggtggc gacgcgcgcc
     6061 ggaataccta acgaacgcgc catcaccgcc atggccgaag catagtaatc gcagtaacct
     6121 ttcttcgcat caaacaacac gtaatcgacg ccgtcctgtc cagcctgcgg gccgggaatg
     6181 gcgtcattgt aaacaatgtt ggtgcgcagc catgcttcca acgccgccgc ttgatcgaac
     6241 gaattcgcgg cgttcgcttt cgtcacgatc tgccgcgcca gatcgcgcac acgactcggc
     6301 agcgaattgg gcagttgcaa ataccgatcg ctgatcgaac gcggatagac cgtcccggcc
     6361 gcgcgcagct gatcggtggt ggcggtgctc aagctcgaga cgactcgata agagtcgcca
     6421 tcattcagcg gctggatcga agtccagatc gtcgcatcga cattcgacgt gctcggcagc
     6481 gtttcaaccc agaccggtcg attcacggcg atgggctcag gtgcggcgaa gatcaaggta
     6541 ttgttaggaa agtgcagagt gaaagtctgc gagatcgcca cgcgctgatc gtaattggcg
     6601 gtcagtggtg gatcagttgg attcaaagtc aaacgctgcg attcactgct ctgccagccg
     6661 ttgctggtgt attcatcata gaccacggct tgccagtagc gacccgcgtt ggcacggacg
     6721 accatcacga cttcggtccc gagagtgcgc gcgccgagca gcgccaacgt tcgaccgaaa
     6781 ggattggcgt acggaatgcc gtgcggctca agaccggaga acatccggct gaaagaatcc
     6841 tcaaccgatc gcagtggccg gctgatctcg tgccacagat cggaaatctg cggcgcgcca
     6901 aagaccgtcg gcgcaacggc gccaaataac accgccgcca aggcgatgcc cgaaccaatc
     6961 cgcaaaaagt cgcgttgaat ctcggagctg tgtcccacgc ggccgctggc ccaacgctgc
     7021 tgctggctca aggtatacat gcgcgcggca tataacaaca cacagaaaat gtagacaatg
     7081 accaggctgg tcaacggtcg atcgccgccg tagtaaaaag tgttgactaa caaaatgacg
     7141 ccggccggag cgaccgcgcg ccacaactgt ttgtatcgaa aagtattcca ggccgcgctg
     7201 aaactgatca accaaaagat cgcggccagg aacatcaaga agatcgaatt gtctttgccc
     7261 acgccgccgg acgcgatgga attgaaccac aaccagatat gctcgcccaa tcgaacgatc
     7321 atcaagcgca gcgtatccag ccacgtccag ccataaatct tgtccggcat ttgatcgatc
     7381 gtgatccacg ccaccatcgc cacaccatac agcagcatga cgatgtgcgc cgttcgagtg
     7441 cgccagcggc gcgtggcaat gaccgcgctc aacagcagcc cgatgacgac gacgttgccc
     7501 aatagatcga gccgcggcat ccactccgcc gaagccacgc tccacgcggc actgaacacg
     7561 gccgcgccga ctaatatcag cgcgaggcga tcgatcggat agggtgattc aagcagatta
     7621 cgtttcatac aaaatgatga gtaacgagta agatgtgctg tgcttaaata cgcacggagt
     7681 tcaacaaagg ttcaaccgca gtgattgtat ctgcatgaag aagaagcgtc aattgatgaa
     7741 ggatgagggc ggagcgagaa atgatacgaa agttgcgagt gatgcacgcg aacccccacc
     7801 atcgccgccc tcgcttcgca cgccctgtcc aactctccac aggagagttg gggtgcacta
     7861 ggtgggggcg gagccattgc ctcgcccctg tcgatgcgcc tttcatcgac ggggggattg
     7921 agagggggtt cttatcgatt ccacctcgaa ggttaatccg gcggggactt cccttcattc
     7981 tacccttgca tcgatcgcat ccgcgcatca tcaataatat tccaaacaaa aactcgacct
     8041 gcatcagagg ccgagttttt tctaatgagt ttcattgaga gaacgaggtg gaagatttct
     8101 gcgcgggctt acacatcacc caatgcagcg caccacgtca gtgccgggaa atgagcggta
     8161 aaaacatttg cgggcgcggg ctcatctccg aactgttgcc accaccatca aaggtagtga
     8221 aggtcacatc cagacgggtg agactattgg gcaaattcac actccattca ccggaagagt
     8281 ttgccaacac gttcgtccga aacaggccgc caccgcccgg tgctgccgga ttgccaacgg
     8341 ctttgtaaat gtagacagcg cagctcgggc aagtcgtgcc ctgcacgagg ctgccgctga
     8401 aggccgtcac aaccgggtac ttcagccaat tgtttggacc caccgatccg tgcgatccgt
     8461 tgaccgttgc gccatcattg cccaagtcaa tcggcaagcc gccgttgcga taaatgtcat
     8521 tattctcaag caaggcgtga gtggagctgc ccgttatggc aatgcccgcc gcgtgattgt
     8581 acgccacgag gttgtccgac agaaagaggc cggagccgcc agtgataaga ataccttcat
     8641 gtgcgttggg taggggcagc aagccgttgg cactggtgcc gatcttgtta ccgatgatcg
     8701 attgggtact gccgctgcta atggccagac cccagtaatt cgcggacaac gtattgaagc
     8761 tcacatcgat attggttcca tcaacgatga tgccgccgtt ggcattatgg gcgatcaggt
     8821 tgtagcggat cgcgatctga tcggagttca ccgcgacacg cacacccgcg taaccgttac
     8881 ctgcggcacc gaggccatct gaggtcgtac cgatatagtt ggcatggttc gtcaagtcat
     8941 cccgttgttg acccacgtac acgtagttgg aattaacaat ctcaacacca gagccgccgt
     9001 gacagcttat cgtattagca tagatgtaag caacgccgtt atcatgactg cccccgctac
     9061 ccgcacggtc gttcaccacg tcgacgccga ctgtcgactg agagaggcta ccgcactggg
     9121 tcgcgttcgg tagaataccc aggtagtcat aggaaatttc tatatctttg ccacccgcaa
     9181 tggatatatc tgcaccagaa ggaatgttga cgactttgat attggagatc gtcacgtaat
     9241 tggcattaat ggttaagcca ttattgcccg tccacaatcc ggcaaaggag gcatcgatgc
     9301 gcgggcagac gcaaccgtta ctgagatcat ggccgtcaat ccaggtggaa tcgcccgaaa
     9361 tagttggcag cgcgctttgc agcagcacgg cgggcagacc cggcgcgaac cggatccacg
     9421 tgccgggatt ggcgttggca tagttgatcg cgcagcgcaa ggtaagaaaa cctgagcaga
     9481 cattctcatc gctgccagcg tcaacaacga tagtgctcgc aggcgctgcc tgcgccgcgt
     9541 gagatgcatt cgccaacacg ggcgcgctca cgccgaatag
//
LOCUS       DASRTJ010000221         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_240230,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000221 DASRTJ010000000
VERSION     DASRTJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240230"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFF70_04670"
     CDS             complement(<1..377)
                     /locus_tag="VFF70_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZY44025.1"
                     /translation="MKELDTTLKKLAKDTEPISIASLYTFSGIDQTAAEQINRVWPTI
                     PTDRRQATMKHLVEIAEENFEVDFNSVYRIGLSDPDPEVRAAAIDGIWEDNDPALIAP
                     LIRFVQQDTTEAVRAAAATGLGQ"
     gene            complement(400..1701)
                     /locus_tag="VFF70_04675"
     CDS             complement(400..1701)
                     /locus_tag="VFF70_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZY44026.1"
                     /translation="MSFGQLVIAFFVVLSPLIFVHELGHFIAAKRGGIRILEFGMFYP
                     PRLFRLWRGKGSMLIGSRRVVIPRNFKLPADLADGKVVRATAVKVNDRLILQSIEVLA
                     DQRIGAPQSEELSIDEAKMYGEVSEFDPGTEYTFNLLPLGGFVRMLGEEGSSGKGSFA
                     EAPKRWRAITLLAGPGMNIIAALVVFMAAFMLGWPKVADRVDVTVSGLAPNSPALIAG
                     LQTGDIIRAVNGVQIDHIDQLQVLITDNAGKSVNMTLERKGQSITVSLTPRTKDQIPQ
                     GQGSLGVTLANNSFIIEQHPLGDAFVNSLDQMRQIVKQITSLPSLLLQGAFASGQARL
                     TGPVGISQMASEAIDLTAQTGQAFWILQLIAAISMALAVTNLLPLPALDGGRLIFVIL
                     EWIRGRKVKPENEAKVHLVGLATLLLLMVLITIQDIQNLPH"
     gene            complement(1903..2874)
                     /gene="mvaD"
                     /locus_tag="VFF70_04680"
     CDS             complement(1903..2874)
                     /gene="mvaD"
                     /locus_tag="VFF70_04680"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HZY44027.1"
                     /translation="MKATAQAHPNIALIKYWGNRDHALRLPANPSLSMNLDGLLTTTT
                     VEFDPALNSDQLLLDGKEVIGAGLDRVSGFLDRVRQLNESKLFARVESENNFPSGAGI
                     ASSASAFAALAVAASAAAKMNLDEAQLSRLARLGSGSACRSVPGGFVEWALGTDDRSS
                     YARSIAPADHWDLTDLIAIVDMGHKSVGSTEGHSLANTSALQAARVASAPDRLARARS
                     AVLKRDFDSLTKVVELDSLMMHAVMMTSKPSLMYWQPATIAVMQAVIDWRRAGLSACA
                     TIDAGPNVHVLCLAQDTEQVTQRLQKISGVRKILSAKAGGPARLIET"
     gene            complement(2974..3294)
                     /locus_tag="VFF70_04685"
     CDS             complement(2974..3294)
                     /locus_tag="VFF70_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY44028.1"
                     /translation="MENLSAQVLHPIDLISYQDGSVVSRTIVNQKAGTVTLFAFDRSQ
                     ELSEHTAPYDALVNLLDGEAKITISGQPHHLKTGDMIILPANQPHALQATQRFKMILT
                     MIRS"
     gene            complement(3313..3984)
                     /locus_tag="VFF70_04690"
     CDS             complement(3313..3984)
                     /locus_tag="VFF70_04690"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZY44029.1"
                     /translation="MRLPCNYAAHNNNNEANMKHLSIPINNETIHDLKVGDPVLLSGV
                     MITARDAAHKWLHETFIKQTREPQGDDLEVYEALKQILISGAIYHCGPVVAGLDTQQY
                     QFVAAGPTTSTREEPYQADVMRHFNVKAVIGKGGMGAKTLQGCQDTPGVYLHAIGGAA
                     SLIAKTVSKVIGVHKYSFGVPEAMWVIEVKDFPAVVTMDSHGESLHAVIEARSKAELD
                     RLLAA"
     gene            complement(4024..4896)
                     /locus_tag="VFF70_04695"
     CDS             complement(4024..4896)
                     /locus_tag="VFF70_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZY44030.1"
                     /translation="MRDLTEQLIELIRRTSTDLPPDIEKSLVMAREGEATGSAAQSAL
                     GTILENVELSRRNSTPICQDTGTPIFTVKYPAGWSTRQLRDQIRNAIVEATKHSYLRP
                     NAVDAITDRNSGNNLGDDAFPTIHFEEIEGDEVIVDLMLKGGGCENVGAQYSLPNSQL
                     GAGRDLAGVRKVVLDAAQKAQGQGCAPGILGVAIGGDRGSSFYASKEVLFRKMDEHSP
                     DPKLAELEDRLTGEANQLGIGPMGFGGETTVLGVKATGLHRLPASFFVSISYMCWANR
                     RRRMIVRGEDTIYE"
     gene            complement(5013..>5744)
                     /locus_tag="VFF70_04700"
     CDS             complement(5013..>5744)
                     /locus_tag="VFF70_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY44031.1"
                     /translation="VCYCRHKMEHVDRDCEAPKDICMTFNGTADSLIRHGFARRVDRS
                     EGIDLLQEAYANNLVQFGENVREGVNFICNCCGCCCEAMIAARKFGLMNPVHTTNFLP
                     SLQADQCNGCGKCVSVCPVEAMTLVSANAAHHPKMKLAKLDAEICLGCGVCTRACTKD
                     ALHLESRPDRVITPINSVHRVVMMAIERGHLQNLIFDNQALFSHRAMAAILGVILKLP
                     PLKQALASKQMKSRYLEHLISRVGV"
BASE COUNT         1309 a         1530 c         1524 g         1381 t
ORIGIN      
        1 aattgtccca gccctgtcgc tgcggcggcg cgtaccgcct cggtggtatc ctgctgcaca
       61 aaacggatca acggtgcgat cagtgccggg tcattatctt cccagatgcc atcgatggcg
      121 gccgctcgca cttcgggatc aggatcagat aagccaatgc gatagaccga attaaaatcc
      181 acttcaaaat tttcttcggc gatctcgacc agatgcttca tcgtggcttg acgtcgatcg
      241 gtggggatgg tcggccacac ccgattgatc tgctcggcag ctgtctgatc gatgcctgag
      301 aaggtataca aactggcaat cgagatcggt tccgtatctt tagccaattt tttcagagta
      361 gtgtcaagtt ccttcatgct tttattcaac cgcggctgat tagtgcggca gattctgtat
      421 gtcttgaatc gtaatgagca ccattaaaag caacagtgtt gccagaccta cgagatgcac
      481 tttcgcttca ttttccggtt tgactttccg accgcgaatc cattccaaga tcacaaagat
      541 caagcggccc ccatcgagcg cgggcagggg tagcagattg gtaacggcca gcgccatact
      601 gatcgccgcg atgagctgca agatccaaaa tgcttgcccg gtctgagccg tcagatcgat
      661 cgcttcgctc gccatctggc tgatgccaac cgggccggtc aaacgtgctt ggcctgaagc
      721 aaatgcgcct tgcaataaaa gacttggtaa cgaggtgatc tgttttacga tctgtctcat
      781 ttgatcgagc gagttgacga acgcatcgcc gagcgggtgt tgttcgatga tgaaggagtt
      841 attcgccaac gtcacgccga gcgacccttg accctgtgga atttgatctt tggtacgagg
      901 cgtcagactg accgtgatcg attgtccttt gcgctcgagt gtcatattga ctgattttcc
      961 ggcattgtct gttatcagca cctgcagctg atcgatatga tcgatttgga cgccgttgac
     1021 ggcgcggatg atgtcgccgg tctgcaagcc ggcgatcagg gcgggcgaat tcggtgcaag
     1081 gccgctgacg gtcacatcga ccctatcagc cactttcggc caacccagca tgaaggcggc
     1141 catgaacacc accagtgcgg caatgatatt catgcccgga ccagccaaca acgttatcgc
     1201 ccgccagcgt ttgggggctt cagcaaaact acctttcccg ctcgatccct cttcacccaa
     1261 catacgcacg aagccaccga gcggcagcaa gttgaatgtg tattcagtgc cgggatcgaa
     1321 ttcgctgact tcgccataca tcttcgcctc atcgatcgat aactcttcgg actgcggcgc
     1381 accgatccgt tgatcggcca acacctcgat cgattggagg atcaatcgat cgtttacttt
     1441 aacggccgtg gctctcacca ctttgccatc cgccaaatcg gcgggcagtt tgaagttgcg
     1501 cggaattacg acgcggcgcg atccgatcag catcgatcct ttaccgcgcc acaggcggaa
     1561 caggcgcggc ggataaaaca tcccgaattc gagtatgcgg attcccccgc gtttagcggc
     1621 aatgaagtgg cccagctcgt gcacgaaaat cagcgggctt aggaccacga agaacgcaat
     1681 gactaattga ccaaatgaca ttcgctaacc tcatctttct tgaataacag aaagattata
     1741 ccccaacatg gaaatcaaca atgatcaatt agcaatgaac gatggcagat gaccaataac
     1801 caatgaaaaa tgacaaatga acaatgacaa agcacgagca tcctgagcgg agaattgaag
     1861 gcgcagactt caactcgtag tcgaaggacg ctttcatcat ttctacgtct cgattaatct
     1921 tgccggccct ccggctttgg ctgagaggat ttttctaaca ccagaaattt tttgcaagcg
     1981 ttgtgtgact tgttcagtat cttgagccag gcacagcaca tgcacattcg gtccggcatc
     2041 gatggtggca caggcagaca agcctgcacg ccgccaatcg atcacggctt gcatcacggc
     2101 gattgtcgct ggctgccagt acatcaagga tggcttagag gtcatcatca ccgcatgcat
     2161 catcaatgaa tcgagttcga caactttagt caatgaatcg aagtcgcgtt tgagtacagc
     2221 cgatcgagcg cgcgccaacc gatcgggcgc ggaggcaacg cgcgcagctt gcaatgcact
     2281 ggtgtttgct aatgaatgtc cttcagtcga tccaactgat ttgtgcccca tatcgacgat
     2341 ggcgatgaga tcagttaagt cccaatgatc ggcgggagcg atcgatctcg cataacttga
     2401 tcgatcatcg gtgcccaggg cccattccac gaagccgccg ggcacgctgc gacaggccga
     2461 gcctgacccc aaacgggcga ggcgcgataa ctgcgcttca tcaagattca ttttcgcggc
     2521 ggcgctcgcg gcgaccgcta aggctgcgaa ggctgaagcc gatgaagcaa tgcctgcgcc
     2581 cgatggaaaa ttattttcgc tttcaacgcg cgcgaataat ttcgattcat tgagttggcg
     2641 gactcgatcg agaaagccgc taacccgatc gagtcccgca ccgatcactt ctttgccatc
     2701 gaggagcagt tgatcggagt tgagtgccgg atcgaattca accgtggttg tggtcagtaa
     2761 accgtcgagg ttcatcgaca acgatggatt tgctggtaac cgcagggcgt gatcgcgatt
     2821 gccccaatat ttgatcaggg cgatgttggg atgggcttga gcagtggctt tcattaagga
     2881 gcaagtttac cacgctcgag gtcgggcgga cattcgattg taatctagtg ctacagtaag
     2941 aagatgaaac gaggctgttt aatttagttc agatcacgat cggatcatgg tcaagatcat
     3001 cttaaagcgc tgcgtcgcct gcaaggcgtg cggttgattc gctggcaaga taatcatatc
     3061 accggttttg agatgatgcg gctggcctga aatcgtaatc ttagcttcac catctagcag
     3121 attgaccagc gcatcgtaag gtgcagtgtg ttcgctcaac tcttgacttc gatcgaacgc
     3181 gaacaaagtc actgtcccgg ccttttgatt gacaatcgtc cggctgacaa ccgatccgtc
     3241 ttgatagctg atcagatcga tcgggtgtag cacctgtgct gaaagatttt ccatcgatgt
     3301 gctcccttcc gtttaagctg ccaacaaccg atcgagctcg gccttcgatc gggcttcgat
     3361 caccgcgtgc agactttcgc catgcgaatc catcgtcacg accgcgggaa aatctttcac
     3421 ttcgatcacc cacatcgcct ccggcacgcc gaaagaatat ttatgcacgc cgatcacttt
     3481 cgagacggtc ttggcgatca atgaggctgc gccaccgatc gcgtgcagat aaacgccggg
     3541 cgtatcttgg caaccttgca aagttttagc gcccatgcca cctttaccga taacggcttt
     3601 gacgttgaag tggcgcatga catcggcttg atacggctct tcgcgcgtac tggtggttgg
     3661 accggcggcg acgaattgat attgttgcgt atcgagtcca gcgacgaccg gaccgcagtg
     3721 atagatcgcg ccactaatga gtatctgctt gagcgcctca tatacttcga gatcgtcgcc
     3781 ctgtggttcg cgtgtctgct taatgaatgt ctcgtgcagc catttgtgtg cggcatcgcg
     3841 cgccgtgatc atgacgccgg ataacaagac tgggtcgccg actttgagat cgtggatggt
     3901 ttcattattg atgggaatgc ttagatgttt catgtttgcc tcgttgttat tattgtgggc
     3961 agcgtagtta catggcaatc tcacctgaga ttgcttcgtc ggtgcgctcc tcgcaatgac
     4021 gttttattcg taaatagtat cttcaccgcg cacgatcatt cttcgtcgcc gattagccca
     4081 gcacatataa gagatcgaca cgaagaaact ggcgggaagt cgatgcaggc ccgtcgcttt
     4141 tacgccgagt accgtcgttt cgccgccgaa gcccatcgga ccgatgccta attgattggc
     4201 ttcgccggtt aatcgatctt caagctcagc caatttagga tcggggctgt gctcatccat
     4261 tttacggaag agtacttcct tcgatgcgta aaacgatgag ccgcgatcgc cgccgatggc
     4321 gacaccgaga atgcccggcg cgcagccttg cccctgcgct ttctgcgcgg catccaatac
     4381 aactttgcgg acaccggcta gatcgcgtcc cgcgccgagt tggctgttag ggagtgaata
     4441 ttgtgcgccg acattttcgc aaccgccgcc tttcaacatc agatcgacga tcacttcgtc
     4501 accctcaatc tcttcgaagt gaatcgttgg gaatgcgtca tcacccaggt tattgccgct
     4561 gttccgatcg gtgatcgcat caaccgcatt aggtcgcaga tacgagtgct tggttgcttc
     4621 gacgatcgca ttgcggattt gatcgcgcag ctggcgtgtg ctccagcccg ccggatattt
     4681 gacggtgaag atcggcgtgc cggtgtcctg acagatcggc gtggagttgc ggcgagagag
     4741 ttcgacgttt tcgagaattg tgcccagcgc gctttgtgcc gccgagccag tcgcttcgcc
     4801 ttcgcgcgcc atgaccaacg atttctcgat atcgggcggc agatcggtcg aggtgcggcg
     4861 gatcaattcg atcaattgtt cagttaaatc gcgcatactg tttctcctca agaaaaggcc
     4921 gtgtgtttct attttattct aagcaaaaca agggacgaat ggtaatgatg attgtcaatc
     4981 aatcactgga catttgtcct tcaattacaa cttcagacac caactctgga tataagatgt
     5041 tccaaatatc tggacttcat ttgcttgctc gccaatgctt gtttgagagg tggcagtttc
     5101 agaataacac cgaggattgc cgccatggcg cgatggctga acaatgcctg attgtcgaag
     5161 atcaaattct gcaagtgacc gcgttcgatc gccatcatga cgacgcgatg aacggaattg
     5221 atcggcgtga tgacccgatc gggccgcgat tccagatgca gcgcgtcttt ggtgcaggcg
     5281 cgggtacaca cgccgcagcc gagacaaatc tccgcatcga gtttggcgag tttcattttg
     5341 ggatgatggg cagcgttggc cgagaccagc gtcatcgctt cgaccggaca gacgctgaca
     5401 catttgccgc agccgttgca ttgatcggct tgaaggcttg gcaggaaatt ggtggtgtgc
     5461 accggattca tcaaaccgaa tttgcgcgcg gcgatcatcg cttcgcagca acagccgcag
     5521 caattgcaaa tgaagttgac accctcgcgt acgttttcgc cgaactgcac caagttattg
     5581 gcatacgctt cttgcagaag atcaatgccc tcagatcgat cgacacggcg ggcgaagccg
     5641 tgacggatca acgaatcggc cgtgccgttg aaggtcatgc aaatatcttt gggcgcttca
     5701 caatctcgat cgacatgttc cattttgtgg cggcagtagc acac
//
LOCUS       DASRTJ010000222         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_240258,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000222 DASRTJ010000000
VERSION     DASRTJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240258"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..832
                     /locus_tag="VFF70_04705"
     CDS             95..832
                     /locus_tag="VFF70_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY44032.1"
                     /translation="MANQVKKYGFVIDPKRCIDCRACLVACRAENNVPLDHTRIWVYD
                     QGVQGQFPNLSQQFVPYNCMHCDEPPCVEACPSQATYKRPEDGIVVIDQEACIGCGLC
                     IEACPYHARYMNPTTGKADKCNACLQRTQQGEAPACVATCVGGSRLFGDLNDPNSEVF
                     KALREAKTAKRLKAIGSDTGPNIYYINEKVDEQSLAITSAVMPAADDFYKKFVIPGVV
                     AAIGATFLGQAVAFAKQLSKGESEFDE"
     gene            924..1727
                     /locus_tag="VFF70_04710"
     CDS             924..1727
                     /locus_tag="VFF70_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZY44033.1"
                     /translation="MSAEVLSLAERRRLRKTKIQKHGLANIVTHWFNVFSWFWLLLTG
                     LGILGNPDLKIMPQGYIDFMRNLFNGIGGLVETHKLFGLIWLFVIIYNVLLGMRKYFV
                     PFAKKRMLLTPDDVQWLKLYGQKLLGRKVTLPPQDAYNAGQKIYGYMVILGTVLIGTT
                     GLMMTFGYLLPADMRWLLQWSRPVHFGAVGMVVAGLVIHVYMAAFFPEEKSAFFSMFT
                     GEVDALYARLHHKKWYDEKLAEERDYEMRVRLEEIEAKEAALAEVDRPA"
     gene            1751..>2181
                     /locus_tag="VFF70_04715"
     CDS             1751..>2181
                     /locus_tag="VFF70_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZY44034.1"
                     /translation="MVTTPTKIDWIEAPSVYDRSLIDLLSESAARHKHLCPRQVLGVR
                     MGLFAAHELQIDLPRNDKRLLIIVETDGCFVDGLSAATGCSIGHRTLRLIDHGKIAAA
                     FIDTETNQTVRIVPKRSARQAARQYGPKAQSRWHAQLEAYQ"
BASE COUNT          540 a          559 c          604 g          478 t
ORIGIN      
        1 agcgatctgc atgtgacctt caccgatccg gtcagcggcg gacaggcttt gtcgcaaaca
       61 ttcgtcgcag ttgagaaagc gtaaggggta aagcatggcc aatcaagtca agaaatatgg
      121 ttttgtcatt gatcccaaac gctgcatcga ttgtcgcgcc tgtctggtcg cgtgccgcgc
      181 cgagaataac gtgccgctcg atcacacgcg catttgggtt tacgatcaag gcgtgcaggg
      241 acagttcccg aatttgtcac agcaattcgt gccctacaac tgcatgcact gcgatgaacc
      301 accgtgtgtc gaggcgtgtc caagtcaagc gacatacaag cgaccggaag acggcattgt
      361 cgtgatcgat caagaagcct gcatcggttg cggcttgtgt atcgaggcgt gtccgtatca
      421 tgcgcgctat atgaatccga cgacgggcaa agctgataaa tgcaatgcgt gtctgcaacg
      481 cacccaacaa ggcgaagcgc ctgcatgtgt ggccacctgc gtcggcggtt cacgattatt
      541 cggcgatctc aacgatccga atagcgaggt cttcaaagcc ttgcgcgaag cgaaaaccgc
      601 gaagcggctg aaagcgatcg gatcagatac cgggccgaac atctactaca tcaacgaaaa
      661 ggtcgatgag cagagtttgg cgatcacatc ggccgtgatg ccggccgctg atgacttcta
      721 caaaaagttt gtcatcccgg gcgtggtcgc cgctatcggc gcgactttcc tgggtcaagc
      781 cgtcgctttc gccaagcaat tgagtaaagg cgagagtgag tttgacgaat aaacagaacg
      841 ctgattgacg ctgataaacg ctgataagaa aaataaaatc agcgcaatca gcgtgtatca
      901 gcatccaata aatggagcgc aagatgtcag ctgaagtttt atccttagcc gaacgaagac
      961 gtttacgcaa aacaaaaatc cagaagcacg gcctggccaa tatcgtgacg cattggttca
     1021 atgtgttctc atggttctgg ctgctattga ccggactcgg catcttgggc aatcccgatc
     1081 tgaagatcat gccgcagggc tacatcgatt tcatgcgcaa ccttttcaac ggcatcggcg
     1141 gactcgtcga gacgcacaag ctctttgggc tgatctggct gttcgtgatt atctacaatg
     1201 tgctgctagg catgcgcaaa tattttgtac cgttcgccaa gaaacggatg ctcctgacgc
     1261 cggatgacgt ccagtggttg aaattgtacg gtcagaaatt attgggcagg aaagtgacac
     1321 tcccgcccca ggatgcatac aacgccggac aaaaaatata tggctacatg gtcatcctcg
     1381 gcaccgtctt gatcggcaca acgggcttga tgatgacctt tggttacttg ctgcccgccg
     1441 atatgcgctg gctgctgcaa tggtcacgcc ccgttcactt cggcgcggtc ggcatggttg
     1501 tcgccggatt agtcattcat gtttacatgg ccgccttctt ccccgaagag aagtcggcct
     1561 tcttctcgat gttcacgggc gaagtcgatg cactgtatgc gcgcttgcat cataagaaat
     1621 ggtacgacga gaagttggcg gaagaacgcg attacgaaat gcgcgtgcgg ctggaagaga
     1681 ttgaggccaa agaagcagcg ctggctgaag ttgatcggcc ggcttaagtc cgattgagaa
     1741 agcgggcgat atggttacga caccaactaa gatagactgg atcgaagcac caagcgttta
     1801 cgatcgatcg ttgatcgatc tactcagcga atcggcggcg cggcacaagc acctttgccc
     1861 gaggcaagtt ctcggtgtgc ggatgggctt attcgccgcg cacgaattgc agatcgatct
     1921 accacgcaat gacaaacgat tgttgatcat cgtcgaaacg gatggctgct tcgtcgatgg
     1981 gttatcggca gccaccggct gctcgatcgg acatcgtaca ctccgtttga tcgatcacgg
     2041 caagatcgcg gcagcgttta tcgataccga aacgaatcaa acggtacgca tcgtgccaaa
     2101 gcgatcggcg cgtcaagcgg cacgacagta tgggccgaag gctcaatcgc gttggcacgc
     2161 tcaacttgaa gcctaccaga t
//
LOCUS       DASRTJ010000223         5716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_88576,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000223 DASRTJ010000000
VERSION     DASRTJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88576"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2421)
                     /locus_tag="VFF70_04720"
     CDS             complement(<1..2421)
                     /locus_tag="VFF70_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY44035.1"
                     /translation="MKIKPLPFSKLRRVADPRRLKFKTTDELSVVDYIFGQPRAVRAI
                     DFGIGVRGLGFNVFVTGASGTGRMSTVARFLKKHAATQPIPDDWVYVHNFSDPLRPRA
                     MSLPPGRAQRFKQDMLAVMNLLKKDLSAIFDTAEYQQAASKITRLADEARGHLFDQLE
                     AILNPAGFLLARSDQGLFVAPEGKAGKPLSPQKLAALPLDRQEELARAQPDMEAALSS
                     TLRSVHAIDQTVRAQLQDLDRQTATRVIAHALDELIDRYRVDSTEAADYLTEVQRNII
                     DHVAEFRSKPEDQSGSPADDQAAEPDPMIRFEVNVIVDHSRTQGVPVIVETNPTFMNL
                     IGRVERDLRYGDQVLDFTMLRPGALHRANGGYLIVRAQDVLKDANAWEALKRALSNRR
                     IEIEDPGTQLQMFSTRTLQSEPVPLNVKVILMGSHRVYYDVYEQDEDFQQLFKVRADF
                     ASAMARLPKNEHAYAQFICARCAELQLPPFDAGAVAQIIEYGSRLAEDQTKLSTRFGA
                     VTDVIIEAAHWADSNFRPHVTADDVRQSLNEARYRSSLAEEETRQQITDGTLNIQTSG
                     AVVGQINGLSVVEIGEYEFGQPARISVRAYVGRNGVISIEREAHLSGRIYNKGVLILE
                     GYLGGQYATDRPLTLAASISFEQNYSEVEGDSASSAELYALLSSLANLPIAQSIAVTG
                     AVDQRGHMMPVGGVQYKIEGFFDVCQARGLSGDQGVLIPVTNIKHLMLREDVVEACKK
                     KRFHIWAVNTIDEGLELLTGVTAGRRSVRGTFPKESVHGRVQARLKSIADRLDGRDKD
                     RRDEKKQDR"
     gene            complement(2540..3676)
                     /gene="dnaN"
                     /locus_tag="VFF70_04725"
     CDS             complement(2540..3676)
                     /gene="dnaN"
                     /locus_tag="VFF70_04725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZY44036.1"
                     /translation="MKVSCLQENLAKGLSIVSRAVAARSTLPVLGNILLATDDSRLKL
                     SATNLEIGITCWIGAKVEEDGATTVPARTFVDLVNALPQDRVDMELAIKTQTLNVICG
                     RFANNVKGVDAQEFPIIPTPDEHNSIHIAPDILRKMIEQVVFAAATDEARPILTGVLA
                     KFSGKSLTFAAADGFRLSVRTAQLEDGVDEPLEVIIPARALAELGRILGDQTDPISMT
                     VTPQRSQILFHLSNIDLVSQLIEGKFPDYNAIVPKSTATKSIVDTGLLLKACKAANVF
                     AREAANIAKVTITPGDQLTPGHITIGAQSAETGDNAGELDASIDGTPIEIAFNVKYLI
                     DVLNVVGTDRISLETVDPSKPGLIRPIGDETFTHVIMPMHIGGR"
     gene            complement(3803..4711)
                     /locus_tag="VFF70_04730"
     CDS             complement(3803..4711)
                     /locus_tag="VFF70_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44037.1"
                     /translation="MTHQPPVLDYEGSKYSTEFWNPDREYEDRAERIAIRSMLPPSGR
                     RLIEIGAGAGRLGELYHGYAEVILMDYARSTVVEARDRWHDDPRFKFVAADLYQLPFA
                     ASAFDSIVMIRVIHHLVDVPRALNEIAAVMKSKGVFLLEFANKRNLKSMARYALRQQQ
                     WSPYDRSPIEFVKLNFDFHPTWLKQQLRSAGFSIEKTRSVSMFRLGALKRLFGAQRLA
                     AIDGALQRPLAPLALSPSMFVRCRGGRPSSLRYEDLRPSAPTETLFKCPACREPLTSE
                     DTELRCVNVHRWSTAEGIFDFRSPIR"
     gene            complement(4814..>5716)
                     /locus_tag="VFF70_04735"
     CDS             complement(4814..>5716)
                     /locus_tag="VFF70_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44038.1"
                     /translation="IYAPASQVQPPSGMLLIYVCANQKDTLIYSDVAVDPQPMTQVFR
                     VDNLIGAKHGGQLATLSSICGAHTCFDTFNILGWNGRAFVSLMAEPLTLPSAGYVLSK
                     KDTETAYHIEAQGGVVASTGAGPQRSDTQVWKWNGAQFAKVSSTLSPIEYRIHAIYEA
                     DDAFAKNDYQSAVDGYSRAITDDSLKDWLTEIGYSKAHDRATLTAYARFRLLLIGVLR
                     GDANASDQLDQLTNDFPANNPDHIIQQMASIFWSKYQATKDIKTACAAADAFANDKYQ
                     ITDNLSLFGYANRWYTSDDMCPIK"
BASE COUNT         1288 a         1586 c         1546 g         1296 t
ORIGIN      
        1 cctgtcctgc tttttctcgt cacgccgatc tttgtcgcgg ccatccaatc gatcggcgat
       61 cgatttcaaa cgcgcttgca cgcgaccatg caccgactct ttcgggaaag tgccgcgcac
      121 actgcgccga ccggccgtga caccggtcag caattcaagg ccttcatcga tcgtgttcac
      181 cgcccaaatg tggaaccgtt ttttcttgca cgcttcaacg acatcttcgc gcagcattaa
      241 atgcttgata ttggttacag gaatcaacac gccttgatcg ccgctgagac cgcgcgcctg
      301 gcacacatca aagaaccctt cgatcttgta ttgcactccg ccgacaggca tcatgtgccc
      361 gcgttgatcc actgcgcccg tcacggcgat gctctgagcg atcggcaaat tcgccagact
      421 cgacaacaag gcgtataact ctgcgctcga agcgctgtca ccctccactt cagaataatt
      481 ctgctcaaag ctgatgctgg ccgccaaggt caacggtcga tcggttgcat attgaccgcc
      541 taaatagcct tcgaggatca acacaccttt gttgtaaatc cgcccagaca gatgcgcctc
      601 acgctcgatc gagatcacgc cgttgcgccc cacgtacgct cgcaccgaaa tgcgcgcggg
      661 ttgtccgaat tcatattcac caatctcgac cactgacaag ccgttgattt gcccgacgac
      721 cgcgccgctg gtttgaatgt tgagtgtgcc gtctgtgatc tgctgacgtg tttcttcttc
      781 ggccagactc gagcgataac gtgcttcatt caaggactgg cggacatcat cggccgtcac
      841 gtgaggtctg aaatttgaat ccgcccaatg agctgcttca atgattacat ccgtcacagc
      901 gccaaagcgc gttgacagtt tagtttgatc ttcggccagg cgcgatccgt actcgatgat
      961 ctgcgccacg gcgccggcat caaacggcgg cagctggagt tcagcgcagc gcgcgcaaat
     1021 gaactgagca taagcatgct cgttcttcgg caagcgcgcc atcgccgaag caaagtcggc
     1081 tcgcactttg aacaactgct gaaagtcttc atcctgctcg tacacatcgt aatacacgcg
     1141 gtgtgaaccc atcagaataa ctttcacgtt cagcgggacc ggctcggatt gcaatgtgcg
     1201 cgtggaaaac atttggagct gcgtgccggg atcttcaatc tcaatccgcc gattgctcaa
     1261 cgcgcgtttc agagcttccc aggcattggc atcttttaat acatcttggg cacgtacgat
     1321 caggtagccc ccattcgcac gatgcagtgc gccgggtcgc agcatggtga agtccagaac
     1381 ttgatcgccg tagcgcagat cgcgttcgac gcggccgatc aaattcataa atgttgggtt
     1441 ggtttcaacg atcaccggca cgccctgtgt ccggctgtga tcgacgatca cattcacttc
     1501 aaagcggatc atgggatcag gctcagcggc ctgatcgtcg gccgggctgc ccgattgatc
     1561 ttcgggtttc gatctgaact ctgcaacgtg atcgatgata ttgcgttgaa cttcggtcaa
     1621 atagtcagcc gcttcagtgc tgtcgacccg ataccgatcg atcagttcat cgagtgcgtg
     1681 tgcgatcacg cgggtcgccg tctgtcgatc gagatcctgc agctgcgcgc gcacggtctg
     1741 atcgatcgca tgcaccgagc gcagcgtcga gctcagagca gcttccatat ccggctgggc
     1801 acgcgccagt tcttcctgac gatcgagcgg cagcgcagcg agtttttgag gagagagtgg
     1861 cttgccagct ttgccttccg gtgcgacaaa caagccctga tccgaccggg ctaacagaaa
     1921 gccggcgggg tttaagatcg cctccagttg atcgaaaaga tgcccgcgcg cttcatcggc
     1981 cagacgcgta atcttcgaag cggcttgctg atattcagcg gtgtcgaaaa tggcggacaa
     2041 atcttttttc agaagattca tcactgcgag catatcttgc ttgaaacgct gggcacgtcc
     2101 cggcggcaga ctcatcgccc gcgggcgcag cggatcgctg aagttatgga catagaccca
     2161 gtcgtccggt attggctgag tcgcggcgtg tttcttcaag aagcgcgcca ccgtagacat
     2221 gcggccggtg ccgctggcgc cggtgacgaa cacgttgaag cccagcccgc gcacgccaat
     2281 accaaaatcg atcgcgcgca cggcccgcgg ttggccaaag atgtaatcca cgacggacag
     2341 ctcgtccgtg gttttaaatt tcaagcggcg cggatcggcc acgcggcgga gttttgaaaa
     2401 aggcaggggt tttattttca tcgcgcaact cgctattcac atcatgtcta ttttcgcttg
     2461 aaacaaaaag ccctcagagt ttcatccccg agggcaattc aattttcaat atggtcaatg
     2521 atcgggatta atcgggagct taacgtccgc cgatgtgcat gggcatgatc acatgggtga
     2581 aggtctcatc gccgattgga cgaatgagac ccggttttga agggtcaacc gtttcgagcg
     2641 agatgcgatc ggtgccgacc acattcaaca catcgatcaa atatttgacg ttgaacgcaa
     2701 tctcgatcgg tgtgccatcg atcgaggcat ccagctcgcc ggcgttgtcg ccggtctcgg
     2761 cgctctgcgc accgatggtg atatgccccg gcgtgagttg atcgccgggc gtgatggtga
     2821 ctttggcaat gttagccgct tcacgggcaa aaacattggc cgctttgcac gccttcaata
     2881 acaagccggt gtccacgatg cttttagtcg ccgtcgactt gggaacgatg gcgttgtaat
     2941 ccgggaactt accctcaatc aactgtgaca ccagatcgat gttgcttaaa tgaaacagaa
     3001 tttggctgcg ctgtggggta acggtcatgc tgatcggatc agtttgatcg cccagaatgc
     3061 ggcccaactc ggccaaggcg cgcgccggaa taattacttc cagtggctca tcaacgccgt
     3121 cttccaactg ggcagttcgc accgacaagc ggaaaccatc cgccgcagcg aaggtgagag
     3181 atttgccgct gaacttggcg agcacgccgg tgaggatggg ccgggcttca tcggtagcgg
     3241 cggcaaacac aacttgttcg atcatcttgc gcagaatatc cggcgcgatg tggatcgagt
     3301 tatgctcatc gggtgtgggg atgatcggaa attcctgcgc gtccacgcct ttgacgttat
     3361 tggcgaagcg gccgcatatg acattcaagg tttgcgtctt gatcgccagt tccatatcga
     3421 cgcgatcctg cggcagcgca ttcaccagat cgacaaaggt tcgcgcgggc acggtcgtgg
     3481 cgccgtcttc ttcaactttg gcgccgatcc agcaggtgat gccaatctcc aaattcgtgg
     3541 ctgacagttt aagccgcgag tcgtcggtgg cgagcagaat gttgccgagt accggcaaag
     3601 tcgatcgagc ggcaacggcc cgactgacga tgcttaaccc tttggctaaa ttttcttgca
     3661 ggcaggaaac cttcatgagt gcaacctccg gacaagcaga tggcttagag cggatcgctt
     3721 ttcacatcat gctgtggtcg atagtatatc atcttttggt taacgatcaa agatcgattt
     3781 tgaatccgtt gcggttgatc tcttatctga ttggtgatcg gaaatcaaaa attccttcag
     3841 ccgttgacca gcgatgaacg ttgacgcagc gcagttccgt atcctctgaa gtcagcggct
     3901 cacgacatgc aggacatttg aacaatgtct ccgtaggggc ggacggtcga agatcctcgt
     3961 agcgaagcga agagggccgt ccgcccctac aacgaacaaa catgctcggc gaaagcgcca
     4021 gcggcgcgag tggacgctgc agcgcaccat cgatcgcggc caagcgctgc gcgccgaaca
     4081 aacgtttgag cgcgcccaaa cggaacatcg agaccgatcg agttttttcg atagagaagc
     4141 cggccgatcg aagctgctgt ttcagccagg tgggatgaaa gtcgaagttc aacttcacga
     4201 actcgatcgg tgatcgatca tatggcgacc actgctgctg gcgcagcgca tagcgcgcca
     4261 tcgattttag attacgcttg ttagcgaatt ccagcaagaa cacgcccttt gatttcataa
     4321 cagccgcgat ctcattcagc gcacgcggca catcgaccag atggtggatg acccggatca
     4381 tcacgatcga gtcgaacgcg ctggccgcaa acggcaattg gtatagatcg gcggcgacga
     4441 acttgaatcg tggatcgtca tgccagcgat cgcgtgcttc gaccactgtc gagcgagcgt
     4501 aatccatgag aatcacttca gcgtagccgt gatacagttc gcccaaacgg cccgcccctg
     4561 caccgatctc gatcaagcgg cggccgctcg gcggcagcat cgatcggatc gcaatccgtt
     4621 ctgcgcgatc ttcatattcc cgatcgggat tccaaaactc ggtgctgtat ttcgatcctt
     4681 cgtaatcgag gacggggggt tggtgagtca tggcgataaa ggttggaaag aatatcgaat
     4741 caagcagcaa gtgacgagtg aatagtgacg aatgaaaaac aaaagctgtg cttcactcat
     4801 cactcgtcac tcatcattta attgggcaca tatcatccga cgtataccat cgattggcat
     4861 aaccgaacaa actcaggtta tcggtaattt gatatttgtc gttagcaaag gcatcggcgg
     4921 cggcacaggc tgttttgata tctttagtcg cctgatattt gctccagaag atcgaagcca
     4981 tttgctgaat aatgtgatca ggattgttcg ctggaaaatc attggtcagc tgatcgagtt
     5041 gatcggatgc gttcgcatcg ccgcgcagca cgccgatcaa cagcagacga aaacgcgcgt
     5101 aagccgtcaa tgtcgctcga tcgtgcgctt tggagtaacc gatctccgtt agccaatctt
     5161 tcaatgaatc atcggtgatt gcgcggctat acccatcgac cgcagactgg taatcattct
     5221 tggcgaaggc atcatccgct tcataaatcg cgtggatgcg atactcgatt ggcgacaacg
     5281 ttgaggacac tttagcaaac tgtgcgccgt tccatttcca aacttgcgta tcgctgcgct
     5341 gaggccccgc gcccgtcgag gcgacgacac ccccttgggc ttcgatgtga taggcggttt
     5401 ccgtgtcctt cttgctcaac acgtaacccg ctgaaggcag cgtcaacggt tccgccatta
     5461 agctaacgaa ggccctgccg ttccagccca agatattgaa tgtgtcaaaa caagtgtgcg
     5521 cgccgcaaat cgaggacagc gtagccagct gaccgccgtg ttttgcgccg attaaattat
     5581 caaccctgaa cacctgggtc attggttgag gatcgacggc cacatccgaa taaatcaaag
     5641 tatctttttg attcgcgcag acgtaaatca acagcatgcc gctgggcggc tgcacttgcg
     5701 aggcgggtgc atagat
//
LOCUS       DASRTJ010000224         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_270705,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000224 DASRTJ010000000
VERSION     DASRTJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270705"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFF70_04740"
     CDS             complement(<1..1014)
                     /locus_tag="VFF70_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HZY44039.1"
                     /translation="MNKHANWTPNSIDRVLDRVLLSIEKPGRYTGGEHNSIVKDWTAA
                     KIRVATFFPDIYELGMSNLGLATLYDILNKQANILCERAYVPWTDMEEVMRREGLPLY
                     SLETHHPLIEFDLIGISLPYEQLYSNVINFLDLSRIPILAADRTALDPIVIAGGHATY
                     NPDPMADFIDAFAIGEGEELIVDVARCIEQWKADCGDTRSDLARSKSLRADLLKRLAR
                     IEGMYVPSLYEVTYHSDGTIADVQPLDLDLPEKIRKRIVPVLPPPLTKFIVPYVDIAH
                     NRAPIEIMRGCTRGCRFCHAGMVTRPVRERKVQDVVNAVEEILRETGFEEIGLLSLSS
                     SDYT"
     gene            1280..2260
                     /locus_tag="VFF70_04745"
     CDS             1280..2260
                     /locus_tag="VFF70_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZY44040.1"
                     /translation="MQTDLRGRDLIGDLDFSKEEVETVMEVALDLKRKRALGEPHPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIDSKTTQISHGDTPKEMGEIFGRYF
                     DGIAIRNVDWGIGNGYLNAVAKASRVPILNMQCDIYHPFQILADLMTILEKKGRDLRR
                     KKMVVSWAYAASYQKPLSVPQSLILQMTRFGMDVVLAAPPEYKLMPDIMQQAKDNARK
                     FNVGFEVTDNMEEAFKDADIVYPKSWGCTLTTTDTDEMTKVSKKYESWICDARKMKLA
                     KPDAIYMHCLPADRNIEVSDEVIDGPNSVVYDEAENRMHAQKAVMALTMR"
     gene            2267..3019
                     /locus_tag="VFF70_04750"
     CDS             2267..3019
                     /locus_tag="VFF70_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZY44041.1"
                     /translation="MRTTDLVAYLNEYLRVAEIEDRSNNGLQVEGVGEVNKIAFAVDA
                     CLASFETAHAAQVQMLITHHGLFWSEVKLLSGPHFKRIKSLIDGNINLYAAHLPLDEH
                     TEVGNNVELCRLLGLINVQPWGRYQSGPAGYGGALPQPMKLDDLAIKIDGLLDTQSRV
                     LAFGPQIVDRVAVCSGGASMFIPQVQAAGYSTYFSGETAHQHFHDAKDYGLNVIYSGH
                     YATETVGLKALARHLEQHFNLQTVFIDLPTGM"
BASE COUNT          826 a          804 c          882 g          719 t
ORIGIN      
        1 cgtataatcg ctcgatgaca aagagagcag cccgatttct tcaaagccgg tctcgcgcaa
       61 tatttcttcg acggcgttga cgacgtcttg aacttttcgc tcgcgcaccg gacgcgtgac
      121 catgcccgcg tgacagaagc ggcagccacg cgtgcagccg cgcatgatct cgatcggcgc
      181 gcggttgtga gcaatgtcga cgtaagggac aatgaacttg gtcaacggtg gcggtagaac
      241 tggaacgatg cgcttccgaa ttttttcggg gagatcgaga tcgagcggct ggacatccgc
      301 aattgttccg tctgaatgat aagtgacttc atataaagag ggaacataca tgccttcaat
      361 gcgcgcgagt cgcttgagca gatcggcacg cagcgatttt gatcgcgcga gatccgatcg
      421 ggtgtcacca cagtccgctt tccactgttc gatgcagcgt gccacatcga cgatcaattc
      481 ctcgccctcg ccgatcgcaa acgcatcgat gaagtcggcc atcggatcgg gattgtaggt
      541 cgcgtgaccg ccggcaatca cgatcgggtc cagcgccgtc cgatcggctg ccagaatcgg
      601 aattcggctc agatcaagaa agttgatcac gttgctatac aattgctcgt aaggcaaact
      661 gatcccgatg agatcaaact cgatcagcgg atgatgcgtt tcgagcgaat aaagcggcag
      721 accttcacgg cgcatgacct cttccatatc cgtccacggc acatacgcgc gctcacacaa
      781 gatattagct tgtttattga gaatgtcgta cagtgtggcg aggccgaggt tcgacatgcc
      841 aagttcgtaa atatcgggga agaaggtggc gacgcgaatt ttcgccgcag tccaatcttt
      901 gacgatggaa ttgtgctcgc cgcccgtgta acggcccggc ttctcgatcg agagtaaaac
      961 ccgatcgagt acgcgatcga tcgagttggg tgtccaatta gcgtgtttat tcatttaaaa
     1021 atgctcgctt ggctaccgat tagagttatt ttaccatacc aaaattcatg acttctatca
     1081 ggttgcaaat cggacgttat ctattaaaat agacgggtcg gtcgagcact acaggagcaa
     1141 ggacgattgc catgcgagag atgccgatag aaactgaagc aaaccacagg ccgttgtgat
     1201 cgcgcagcta gtcgcgaata aagcggtctg ttttattttg ccgcggtgac tattcaaatt
     1261 tttaaggagt gagtgtatca tgcaaaccga tttacgcggc cgagatttga tcggcgactt
     1321 ggacttcagc aaagaggaag tcgaaacggt gatggaagta gcgctcgatc tgaagcgcaa
     1381 gcgcgctttg ggcgaaccac atccttattt gcgcgataaa gtgctggcga tgttgttctt
     1441 ctttagtagc actcgcacgc gcggatcatt tgaagcgggt atggctcagc tgggcggcca
     1501 tgcggcgttc atcgacagca agacgacgca aatttctcac ggtgatacgc ctaaagagat
     1561 gggcgaaatc ttcggccggt atttcgacgg catcgccatc cgcaacgtcg attggggtat
     1621 cggcaacggc tacctcaacg ccgtggcaaa agcttcgcgt gtgccgatcc tcaacatgca
     1681 gtgcgacatc tatcatccat tccaaattct ggctgacttg atgacaatcc tggaaaagaa
     1741 gggccgtgat ctccgccgca agaaaatggt cgtatcgtgg gcgtatgcgg cgagttatca
     1801 aaaaccgttg agcgtgccac aatcgctgat tctgcaaatg acccgctttg gcatggatgt
     1861 cgtattggcc gcaccgccgg aatacaagtt gatgccggac atcatgcagc aagcgaaaga
     1921 caacgcccgc aagttcaacg taggttttga agtgaccgac aatatggaag aagccttcaa
     1981 agatgccgac atcgtttatc ccaagtcgtg gggctgcacg ttgaccacga ccgataccga
     2041 tgagatgacg aaggtcagca agaaatacga gtcgtggatt tgtgatgcac gaaagatgaa
     2101 acttgccaag cccgatgcga tctacatgca ctgcctgccg gccgatcgca acatcgaggt
     2161 cagcgacgaa gtgatcgacg gaccaaactc ggtggtgtat gatgaagccg agaatcgcat
     2221 gcacgcccaa aaggctgtca tggcgttgac gatgcggtaa taggtgatgc gaacaaccga
     2281 tcttgtggct tatctcaacg agtacttgcg cgtcgccgaa atcgaggatc gctcgaacaa
     2341 cggtctgcaa gttgagggcg tgggtgaagt caataaaatc gcgtttgcgg tcgatgcctg
     2401 tctggcgtca ttcgagacag cacacgctgc gcaagttcag atgttgatca cgcatcacgg
     2461 tctattttgg agcgaagtta aacttttaag tgggccgcat ttcaaacgca tcaaatcgtt
     2521 gatcgatgga aacatcaatt tgtatgcagc ccatctgccg ctcgacgagc acaccgaagt
     2581 cggcaacaac gttgaactgt gccgcttgct cggcttgatc aatgtgcagc cgtggggtcg
     2641 atatcaaagc ggccccgccg gttatggtgg agcattgccg cagccgatga agttagatga
     2701 tctcgcgatc aagatcgatg ggctgctcga cacccaatcg cgcgtgctgg cctttgggcc
     2761 gcagatcgtc gatcgagtcg cggtatgctc cggcggtgca tcgatgttca ttccgcaagt
     2821 tcaagcggcg ggctactcaa cttatttcag cggcgagaca gcgcaccagc attttcatga
     2881 tgcgaaagat tacggactaa acgtgatcta ctctggtcat tacgcaactg agacagtagg
     2941 actcaaagca ttggcgcggc atttagagca gcacttcaat cttcaaacgg tatttatcga
     3001 tctaccgacc ggcatgtaag taaaccttca cgccgggata acttttcggc aacaaaacat
     3061 tttgagaaca aaagaggaca catggcaagg caagcagtga aaacaaaaag cactaaagct
     3121 gtatccaaga aatcgattcg cgcaaaaact tcgacgcgcc gcaaagttgc cgtcgtggca
     3181 gtcggtggta attcgttgat caaagacgaa gcacacaagt cagtccaagg t
//
LOCUS       DASRTJ010000225         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_119163,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000225 DASRTJ010000000
VERSION     DASRTJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119163"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFF70_04755"
     CDS             <1..184
                     /locus_tag="VFF70_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZY44042.1"
                     /translation="MEHALRDLAEKSGLKLGAFFTPIRVAVTGKTVSPPLFGSIVALG
                     REKTIERLRNAQKLLV"
     gene            322..2001
                     /locus_tag="VFF70_04760"
     CDS             322..2001
                     /locus_tag="VFF70_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZY44043.1"
                     /translation="MSDEETNKPTDFIREAVTEDLKTERYTNVHTRFPPEPNGYLHIG
                     HAKAICIDFSIAQEFGGLCNLRFDDTNPTKEDVEYVDAIKEDIHWLGFTWDDREFYAS
                     DYFDQLYEMAIKLIKKGKAYVDDQSAEEVSKNRGTLTEPGIESPYRTRSIEENLDLFQ
                     RMKNGEFPDGARLLRAKIDMASGNINLRDPAMYRIIHAPAHHRTGSTWCIYPMYDFAH
                     GQSDSIEGVSHSLCSLEYEAHRPLYDWFIKELEIFAPRQIEFARLNMNYTVMSKRKLL
                     RLVQDGHVNGWDDPRMPTLCGLRRRGFTPESIRDFANRIGVAKADSVIDVVLLEACLR
                     DDLNKRAPRRMGVLRPLKLVIDHYPDDQVEEMEVSNSPDDPAAGTRKVKFSKTLYFEQ
                     EDFQEVPQPKYYRLFPGNEVRLRYGYIIKCINVVKNDRGEVIEVHCTFDPATRGGDAP
                     DGRKIKSTIHWVSAEHAIDAEVRLYDRLFNRPDPEAGEGYIEYINTNSLTVLKDCKVE
                     AILANTKPGDRFQFERQGYFCADLDSTPDKLVFNLAVALRDTWAKIDQKQK"
BASE COUNT          499 a          553 c          553 g          411 t
ORIGIN      
        1 gatggaacat gcattgcgcg atttagccga gaaatccggc ttgaaactcg gcgcgttctt
       61 cacaccgatc cgcgtggccg tgacgggcaa gactgtttcg ccgccgctgt ttggctcgat
      121 tgtcgcgctt ggccgcgaga aaacgatcga gcgattgagg aatgcccaaa agttgttggt
      181 ttaatcgacg tagggcgacg cgttcgcaca atgagcttca tcgatcggac tcgctgccac
      241 aatcggccag ccgatctaca acgattcgtt ggcgaaggcg tcgcccttac aaaaatcact
      301 tcggcgtcaa ggaactcttt aatgtccgac gaagaaacaa ataaaccgac cgacttcatc
      361 cgcgaagcgg ttacggaaga tttgaaaacg gaacgttaca cgaacgttca cacgcgcttc
      421 ccgccggaac cgaacggtta cttgcacatc ggccacgcga aagccatctg catcgacttc
      481 agtatcgcgc aagagtttgg cggcctgtgc aatctacgct tcgacgacac caacccgacc
      541 aaagaagatg tcgaatacgt cgatgcgatc aaagaggaca ttcactggct gggcttcact
      601 tgggacgatc gggaattcta tgcgtccgat tattttgatc aactctacga gatggcgatc
      661 aagttgatca aaaagggtaa agcctacgtc gatgatcaat ccgccgaaga agtcagcaag
      721 aatcgcggca cactgaccga accgggcatc gagagtccat accgcacgcg atcgatcgag
      781 gagaacctcg atctgtttca acgcatgaag aacggtgaat tccccgacgg cgcccgcctg
      841 ctgcgcgcca agatcgatat ggcatccggc aacattaatc tgcgcgaccc ggcgatgtat
      901 cgcatcattc acgcgcccgc gcatcatcgc acgggcagca cctggtgcat ctatccgatg
      961 tacgacttcg cgcacgggca gagtgattcg atcgagggcg tctcgcattc gctgtgctcg
     1021 cttgaatacg aagcacatcg tccgctgtat gattggttca tcaaggaact ggaaattttc
     1081 gcgccgcgcc agatcgaatt tgcgcgtctg aatatgaact acaccgtgat gagcaagcgc
     1141 aagttgctgc ggctcgtgca ggacggtcac gtgaacggct gggacgatcc ccggatgccg
     1201 actttatgcg gcctgcgccg gcgcggcttc acgcctgaat cgatccgcga ctttgccaat
     1261 cgcattggtg tggccaaggc tgacagcgtc atcgacgtgg tgctgctcga agcctgtttg
     1321 cgcgatgacc tgaacaagcg cgccccgcgc cgcatgggcg tgctgcgtcc gctcaaattg
     1381 gtgatcgatc attatcccga cgatcaagtc gaggagatgg aagtttcaaa tagcccggat
     1441 gatccagcgg ccggcacgcg taaagtaaaa ttctcaaaga ccttatactt cgagcaggaa
     1501 gattttcaag aagtgccgca gcccaaatac tatcgcttgt tccccggcaa cgaagtgcgc
     1561 ttgcgctatg gctacatcat caagtgcatc aacgtcgtaa agaacgatcg aggcgaagtg
     1621 atcgaagtgc attgcacatt cgatccagcg acgcgcggcg gcgatgcgcc cgatggccgt
     1681 aagatcaaat cgaccattca ttgggtcagc gccgagcacg cgatcgatgc ggaagtgcgc
     1741 ctgtacgatc gattgttcaa ccgacccgat cccgaagcgg gcgaaggcta catcgagtac
     1801 atcaatacga attcgctgac ggtcttgaag gactgcaaag tcgaagcgat tttagcgaac
     1861 acgaagcccg gagatcgatt ccagttcgag cggcaaggct acttctgcgc cgatctcgat
     1921 tcgacgccgg ataaattagt gttcaatctc gcggttgcgc tgcgagatac atgggcgaag
     1981 atcgatcaga agcaaaagta agaatttcac cgcaga
//
LOCUS       DASRTJ010000226         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_13229,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000226 DASRTJ010000000
VERSION     DASRTJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13229"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1326)
                     /locus_tag="VFF70_04765"
     CDS             complement(46..1326)
                     /locus_tag="VFF70_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZY44044.1"
                     /translation="MKRSSSAPHWFLDNLSNMLLAVVLAILVWSVAVQQANPNIERTF
                     PAAILIVEQNLPAGMITYGESVRSVRVTLSAPQSAWDVMTADRLTASIDLSGQPTGTL
                     QLDVRVSTADRTVRVTKIEPAAIALKMEPLAEAQAPVAINVVGEPRLGYAPKPVQATP
                     ASVIVRGPASLVRQVIDVGGQISIQDARTDVSQTIALAPRDANGQALANISLVPSSTL
                     AVVGMQQLGGFRDLAVKIDLHGAVAPGFLIANVSVKPQIVTVFGSPSTLEAQPGFIET
                     EPVTVSGATTDLDNRVRLNLPSGVSMLGDPTVQVTVKVNPIESSVTVQRPASIQGMLP
                     GFTVTLSPESVDVILSGPIARLEALHSEDVQVILNLFNLSLGTHQVTPAVIAPNGITV
                     ASILPATIQVSISEIITPTETITPTRTLTPTPKP"
     gene            complement(1323..2213)
                     /gene="cdaA"
                     /locus_tag="VFF70_04770"
     CDS             complement(1323..2213)
                     /gene="cdaA"
                     /locus_tag="VFF70_04770"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZY44045.1"
                     /translation="MFQRLDWLSLLDIGLVALVFFIVLYLVRDTQAVPLLRGVIILLI
                     VIAILGNTLTLPAFGWLFQRAIPALLIAIPVVFQPELRRALERVGRAGWWLNQSNYKT
                     DGIDRVISVISLACQRMAERRHGALIVLERATGLENYADTGVRMDAEVTSELLQSIFF
                     RNSILHDGAVIIRHNRVLSAACILPLTSSFMSDRRMGLRHRAAIGITEATDAIAVVVS
                     EEHGQISIAHNGRMIRRLDAQRLETVLRAFYQLDRQSAPPGWIMWLRQLLGIRSKPKA
                     VAESTPAKNNTTTSQRAVDG"
     gene            complement(2234..>2921)
                     /locus_tag="VFF70_04775"
     CDS             complement(2234..>2921)
                     /locus_tag="VFF70_04775"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY44046.1"
                     /translation="AIYQRILRLAPMDLTIRSKLIDLLVTYGQIDRAIEQYLALADTY
                     YQLASPEKAREKYLEALGLAPRGTTNRLWSNQIMHKIGEMDAQRGDWKRAVQTYEQIR
                     RNDPGDEKAQVTVLELYYKLQPQRAVQEIDQLIKDYKANGQLLKLLPIVEEQVRLHPQ
                     DVGLVSRAAQICIESGLKTQGFGHLNQLGELQLNAGQTKQAIATLRALIALNPPNVND
                     YRKLLTQIGG"
BASE COUNT          643 a          811 c          828 g          639 t
ORIGIN      
        1 ccgagattct tcgccgctgc gcggctcaga atgacaaaaa gatgtttacg gttttggcgt
       61 cggggttaaa gtgcgtgttg gtgtaatcgt ttcggttggt gtgatgatct cactaatgct
      121 gacttgaatt gtcgcgggca ggatgctcgc cacagtaatg ccattgggcg cgattacagc
      181 tggcgtgact tgatgtgtgc ccagactcaa attgaataaa ttgagaatca cttggacatc
      241 ctcagaatgc agcgcttcca agcgcgcaat cggaccgctc aggatcacat caaccgattc
      301 aggtgaaagg gtgaccgtga aaccgggcag catgccttga atggaagcgg gtcgctgcac
      361 tgtgacgctg ctctcgatcg gattcacttt cacggtaact tgtaccgttg gatcgcccaa
      421 catcgatacg cccgatggca gattcaatct tacccgatta tccagatcgg ttgttgcacc
      481 gctgacggtc accggctcgg tctcaatgaa gccgggctga gcttccaatg tcgatggact
      541 gccgaagact gtcacgatct gcggtttgac cgacacattt gcgatcaaga aacccggcgc
      601 aactgcgccg tgtagatcga tcttcacggc gagatcgcga aagccgccca attgctgcat
      661 accgacgacg gccagcgtgc tgctaggcac gagggagata tttgcgagtg cttgaccgtt
      721 cgcatcacgc ggcgcgagcg cgatcgtctg ggagacatcg gtacgcgcat cttgaatgga
      781 aatttgtccg ccgacgtcga tcacttggcg gaccagcgaa gctgggccgc gcacgatcac
      841 actcgcgggt gtggcttgca ctggcttggg cgcgtaacct aagcgcggtt cacccacaac
      901 attaatcgca accggtgcct gcgcttcggc gagtggctcc atcttcaatg caattgccgc
      961 cggttcgatt ttggtgacgc gtaccgttcg atcggctgta ctgacacgca cgtcgagctg
     1021 tagcgtaccg gtcggttggc cggagagatc gatcgaagct gttaatcgat cggcggtcat
     1081 gacatcccag gcggattgcg gcgcactcaa tgtcacgcgc accgatcgca cgctttcgcc
     1141 atacgtaatc atgcccgccg gcaggttttg ttccacgatc aggattgcgg cgggaaaagt
     1201 gcgttcaatg tttggattcg cttgctggac ggcgacgctc cataccaaga tcgctaaaac
     1261 gaccgccagc aacatgttac ttagattgtc gagaaaccaa tgcggtgcgg aagaggagcg
     1321 cttcatccat caaccgcccg ctgtgaagtc gtggtgttgt tcttggccgg agtggactca
     1381 gctactgctt tcggtttgga tcgaatgcct aacaactgcc ggagccacat gatccagccg
     1441 gggggagcgg attgtcgatc gagttgatag aaagctcgca acactgtttc caagcgctgg
     1501 gcgtccaaac ggcggatcat ccggccgtta tgcgcgatcg atatttgtcc atgctcctcg
     1561 ctgacgacga ctgcaattgc atcggtggct tcagtgatgc cgatcgcagc gcgatggcgc
     1621 aagcccatac gccgatcgct catgaagctg gaagtcaacg gcagaatgca ggccgcggac
     1681 agcacgcgat tgtggcgaat gatcacggcg ccgtcgtgca agatggaatt acggaagaag
     1741 atcgattgca gcaattcgga agtaacttcg gcgtccatgc gcacgccggt gtctgcataa
     1801 ttttccaagc ccgtagcgcg ctccagaacg atcaaggcgc cgtggcgtct ctcggccatg
     1861 cgctggcagg ccaacgaaat gacactgata acccgatcga tcccgtccgt tttgtaattc
     1921 gattgattga gccaccaacc ggcgcggccc acgcgttcca atgcccggcg caattcgggt
     1981 tgaaagacca ccgggatggc aatcaacagc gcggggatgg cgcgttggaa caaccagccg
     2041 aacgctggca acgtcagcgt gttgcccaaa atggcgatga caattaacag gatgatgacg
     2101 ccgcgcaaca ggggaactgc ctgtgtatca cggacgaggt acaacacgat gaagaagacc
     2161 agtgccacca gaccgatgtc cagcagactc agccagtcga ggcgttggaa caaagccgcg
     2221 acttcaccca cagctaaccc ccaatctgcg ttagtaactt ccgataatca ttgacgttcg
     2281 gcggattcaa cgcaatcagc gcgcgaagag tggcgatggc ctgcttggtc tgaccggcgt
     2341 tcaactgcaa ttcgccgagc tgattcaaat gcccaaaacc ttgagtcttc aagccggatt
     2401 caatacagat ttgcgcggca cgcgacacca agcccacatc ttgcggatga agtcgcacct
     2461 gctcctcgac gatgggcaat agcttgagca gctggccgtt tgctttatag tctttaatca
     2521 actgatcgat ttcttgcacg gcacgctgcg gctgcagctt atagtacagc tccagcaccg
     2581 tcacttgcgc tttttcatcg ccgggatcgt tccgccgaat ctgttcatag gtttggaccg
     2641 cacgcttcca atcgccgcgc tgcgcgtcca tctcgccgat cttgtgcatg atctgattcg
     2701 accacaagcg gttggtcgtg ccgcgtggcg ccaagcccag cgcttccaaa tatttctcgc
     2761 gcgccttttc tggactggcg agttgatagt aagtgtcggc taaggccaag tactgttcta
     2821 ttgctcggtc gatttggcca tacgttacca gcagatcgat cagtttcgaa cggatcgtca
     2881 aatccatcgg ggccaggcgc aggatgcgtt gatagatagc c
//
LOCUS       DASRTJ010000227         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_13246,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000227 DASRTJ010000000
VERSION     DASRTJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13246"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFF70_04780"
     CDS             complement(<1..1223)
                     /locus_tag="VFF70_04780"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZY44047.1"
                     /translation="MSDLVAVISWWLVIQILGFAVWPLAFRWLRWLPDRGYMLSKPLG
                     LLLVSYGVWLLANFGIVQNTSGGMIAVLIGVAVTGFFIYRRGRTNQTSGEGRTTDDGL
                     RKWIWDHRALVIAYEILFLIAFVGWSIFRAHNPDLSTTEKPMEFAFFNAIGRSVTFPP
                     HDPWLAGYSIAYYYFGYVMMSVLHQLSGVTAGMAFSLSNSLWFALAGASAFGVVANLI
                     MLSKRHTQRAAIVFGSIGAIMLVLMGNLEGSLELAHANNVGSAAMWQWLDVQEINGPA
                     VQNSPSIPIDQPRGGWWWWRASRVIHDYPPDAISPQLASAINEPPSTNSIYQELIDEF
                     PQFSFLLGDMHPHVLSLPFALVCMALALNLFLGASTGAIKSLTSTSMWPLYALAVGGM
                     SFLNTWDFPIYAFILA"
     gene            complement(1282..>1599)
                     /locus_tag="VFF70_04785"
     CDS             complement(1282..>1599)
                     /locus_tag="VFF70_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44048.1"
                     /translation="ARISINTGLPSIIGWDWHQRQQRSIMPGSVIDNRLNDLRTLYQS
                     TDIIAVTRVIDYYSVHYIVVGALERSYYPPEGLAKFDQMVTSGMLSVAYQNNGDKLYE
                     VVK"
BASE COUNT          445 a          434 c          392 g          328 t
ORIGIN      
        1 gccaggataa aggcataaat aggaaagtcc caagtgttga ggaaactcat gccgccaact
       61 gccaacgcgt ataacggcca catcgatgtt gaggttaaag acttgattgc gccagtcgat
      121 gcacccagaa ataaattcaa agccaaggcc atacacacca aagcaaaggg cagactcaat
      181 acgtgtggat gcatatcgcc gagcaagaaa ctaaattgcg gaaactcatc gatcagttct
      241 tgataaatcg agtttgttga tggcggctcg ttgatcgcac tggccaactg cggactgatc
      301 gcatctggcg gatagtcatg aattacacgc gaggcacgcc accaccacca tccaccacgt
      361 ggttgatcga tcgggattga cggggaattt tgaacggccg gaccattgat ctcttgaaca
      421 tccagccact gccacattgc cgccgagccc acattgttcg catgcgctaa ctccagcgaa
      481 ccttcgaggt tacccatcag taccagcatt atcgcgccga tcgatccgaa gacgattgcg
      541 gctcgttgcg tatgacgttt tgacagcata attagattgg cgacaacccc gaacgcactc
      601 gccccggcta aggcgaacca cagcgagttg ctcaagctaa aagccatacc cgcagtgacg
      661 ccggacaatt ggtgcagcac cgacatcatc acgtagccga agtagtaata agcgatcgaa
      721 taaccggcca gccatggatc gtgcggcgga aaagttacgc tgcggccgat cgcgttgaag
      781 aacgcaaact ccatcggttt ttcggtcgtg ctgagatcgg gattatgcgc acggaagatc
      841 gaccagccga cgaaggcaat caagaataaa atttcgtaag caatgaccag agcgcgatgg
      901 tcccagatcc attttctcaa tccatcgtcg gttgttcgtc cttcgccgct ggtctgatta
      961 gttctgccgc ggcgatagat gaagaagccc gtcaccgcca cgccgatcaa gactgcaatc
     1021 attccaccgg acgtgttctg aacaatgccg aagttcgcga gcagccacac accgtatgat
     1081 accaatagta aaccgagcgg cttgctcagc atgtaacctc gatcgggcaa ccagcgcagc
     1141 cagcgaaatg ccagcggcca aacggcgaaa ccgaggattt gaatgacgag ccaccaactg
     1201 ataacggcaa caagatcaga catagttcat gatcgaatag tcatgagtga agagcggtag
     1261 tgcgatttca ctaatcactc atcatttaac cacttcatat aacttatcac cattgttctg
     1321 gtaagcgacg cttaacatgc cagaagttac catctgatca aacttggcga gtccttccgg
     1381 cggataatac gatcgctcca aagcgccgac tacaatgtaa tgaacgctat agtaatcaat
     1441 gaccctagtg acggcgatga tgtccgtcga ttgatacaac gtccgcagat cattcagccg
     1501 attatcgatc accgagccag gcatgatcga tcgctgctgg cgctgatgcc aatcccagcc
     1561 gatgatcgac ggcaagcccg tattgatcga gatgcgtgc
//
LOCUS       DASRTJ010000228         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_195024,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000228 DASRTJ010000000
VERSION     DASRTJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195024"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFF70_04790"
     CDS             complement(<1..798)
                     /locus_tag="VFF70_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZY44049.1"
                     /translation="MPKTKTKSPLTYKPIDPDIVQLAHQVHANPESILEIYQALQARR
                     GYLDIAAISAVADELKIPAERAYGVASFYSMLTLKSHPEHTLRVCDGVACWLRGSQNI
                     HNAIADTYPDWNVERTSCLGLCDRAPAAWVDDQPCGPIDLDRVRTIRSGWRGEPIDYT
                     QPRPGEIRSMLAQAGLIDPDSIDSAIDSGAYRALDRALQQSPSAIIDEVEASGLQGRG
                     GAGFPVGRKWRFVAQADRSPKYIVCNADESEPLIFKDRVLMDTNPHQL"
     gene            complement(1035..1994)
                     /gene="focA"
                     /locus_tag="VFF70_04795"
     CDS             complement(1035..1994)
                     /gene="focA"
                     /locus_tag="VFF70_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HZY44050.1"
                     /translation="MSATTELQIDALLPGEMAAKAERIGVKKAHLNSLSMFVLAVLAG
                     AFIALGAILSTTVVAGAGPVLPYGVTRLLAGFVFSLGLILVVVGGAELFTGNNLIVMA
                     WAHRTVSTRLLLKNWAIVYTGNFVGSIATAVIMFFTSQYTFGSGAVGAAALSTANAKV
                     GYDFLQAIMLGIMCNALVCLAVWLTFSARTVSDKIMAILPPITAFVAAGFEHSIANMY
                     FIPMGLFIRSGAPASFWTNIGKTAADYGNLTWDRFLINNLLPVTIGNIIGGTLMVGAV
                     YWFVYLRPHATELSTHGGSKPVAIKSTDQALPGPVEVMVNGQR"
     gene            complement(2375..2848)
                     /locus_tag="VFF70_04800"
     CDS             complement(2375..2848)
                     /locus_tag="VFF70_04800"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZY44051.1"
                     /translation="MRPSKDRSMFTRQRLITILALLGLGVSGYLTYTHLFGVAIFCGG
                     ASSCELVNSSRYAFLGPFPVSLLGSIGYLAILVLSFIPTNADRQWPAQLIFGVALIGV
                     MFQLYLTYIEVFVLQAICYWCVASQILVLLIFILSLPRSRPAVEEDAADMIDSSS"
     gene            complement(2835..3497)
                     /locus_tag="VFF70_04805"
     CDS             complement(2835..3497)
                     /locus_tag="VFF70_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZY44052.1"
                     /translation="MKSTRRPVQKKNTSATWWIVGIIAAMAGVALIVLVSLPQAAQQH
                     ALVAGTSLGDQNAPILVEEYGDFQCPACGQFARQTLPQIESKYVSSGKVRFVFHHDAF
                     IGEESIKAGEASECAGEQSKFWEYYDTLFANQGGENVGAFSDANLVGFAQQLQLDTDK
                     FTACLNTGKYRSKVTSDTADANARGVTQTPTLFINGQKVIGALSLQQFELYIGPLLNA
                     AK"
     gene            3692..>3985
                     /locus_tag="VFF70_04810"
     CDS             3692..>3985
                     /locus_tag="VFF70_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZY44053.1"
                     /translation="MDIERQIETLPTKLGQAIQKIEAPKKISLSDVILGGQDGLVNVL
                     GVILGVAAASSDPRIVIAAGLAATFAESISMGAVAYTTTMADRDFYLSEIEREK"
BASE COUNT          961 a         1138 c         1066 g          820 t
ORIGIN      
        1 cagttgatgt gggttcgtat ccatcaacac gcggtctttg aaaatcagcg gctcgctttc
       61 gtctgcattg cacacaatat acttcggcga tcgatcggct tgcgcaacga agcgccattt
      121 gcgtcccacc ggaaagcccg cgccgccacg gccttgtagg cccgacgctt caacttcatc
      181 gatgattgct gacggtgatt gctgcagcgc gcgatcgagc gcgcgataag cgccactatc
      241 gatcgccgaa tcgatcgaat cgggatcgat cagaccagcc tgagccagca tgctgcgaat
      301 ttcgccggga cgcggctgcg tataatcgat cggttcgccg cgccaacccg atcggatagt
      361 gcggactcga tcgagatcga tcggcccgca cggttggtca tccacccaag cggcgggcgc
      421 gcgatcgcac aaccccaggc agctggtgcg ctcgacgttc caatcgggat aagtgtccgc
      481 aatggcgttg tgaatattct gtgagccgcg cagccaacag gcgacgccat cgcacactcg
      541 caaggtatgc tctggatgtg acttgagcgt caacatcgaa taaaatgaag cgacgccata
      601 cgcgcgctcg gccggaatct tgagctcatc agccaccgcg ctgatagccg cgatgtccag
      661 ataaccgcgc cgcgcttgca gcgcctgata gatttccaga atcgattcag ggttggcgtg
      721 cacctgatgg gctaactgaa cgatatcggg atcgatcggt ttgtaggtca gcggagattt
      781 cgttttggtt ttgggcataa gtcggcctca caaatcagtc ttgggtcatt tcgaatcctg
      841 cgaccagctc aggatgaact ccgtgggaaa tctctgcgac aatcaactga gatttctcgc
      901 cgcttcgcga ctctaaatga cagccgaagg ttttgagatt ctttcagcct attcttcttc
      961 gatcgagctc ttacgcgaaa atcattcacg caacttactt gatcatcaac gccgacggcg
     1021 ctgcggcgca cacatcaccg ctggccgttg accatgacct cgaccggacc gggcaatgct
     1081 tgatcggttg atttgatcgc cacaggcttc gacccgccat gggttgacaa ttccgttgca
     1141 tgcggccgca gatacacgaa ccagtacacc gcgcccacca tcaacgtgcc gccgatgatg
     1201 ttgccaatgg tcaccggcag caagttgttg atcaaaaatc gatcccaggt gagattgccg
     1261 taatcggcgg ccgtcttgcc gatgttcgtc cagaaagacg ccggcgcgcc cgatcggatg
     1321 aacaagccca tcgggatgaa gtacatattg gcaatgctgt gttcgaaacc ggcggcgacg
     1381 aaggcggtga tcggcggaag aatcgccatg attttatcgg acaccgtgcg cgcgctgaag
     1441 gtcaaccaca cggctaaaca caccaacgca ttgcacatga tgcccaacat gatcgcctgc
     1501 agaaaatcgt agccgacttt ggcattcgcg gtcgagagcg ccgccgcgcc gaccgcgcca
     1561 ctgccaaacg tatattggct ggtgaagaac atgatcaccg ctgtcgcgat tgaaccgacg
     1621 aagttgccgg tgtacacaat ggcccagttc ttcagcaaca agcgcgtgct gacggtgcgg
     1681 tgcgcccagg ccatgacgat caagttattg ccggtgaata attctgcgcc gccaacgacc
     1741 accaagatca ggccgaggct gaacacgaag ccggctaaaa gacgggttac accatacggc
     1801 aggaccggac ccgcacccgc gacgacggtg gtggataaga tcgcgcccag agcaataaac
     1861 gctccggcta acacggccag cacaaacata ctcagtgaat tcaggtgagc tttcttcaca
     1921 ccgatccgct cggctttcgc cgccatttca cccggcagca acgcatcgat ttgaagctca
     1981 gtagtggcag acatagacag actcctgatc aattttaagt gcacgttaag cttagcgggg
     2041 ccgatcgatc actgacagta tcaaacgtca atgagccagc cggatagatg tcacttgcac
     2101 aaaaaagaag cggcttctgg gcagaagccg cttcaaccct gaccagtgag tcagccaagt
     2161 ttgggccggg acaagagaaa ataggttata gctgtctcac tggtttgact cacttaagta
     2221 tcaccgattc gacgctggct tgccagcgtc gattgtcatc tgatcgatag gccaagcatc
     2281 acaccggttg gctcgacggt acgcacggcg ggctttagcg catgggtacc gcatgggtcg
     2341 gcttctgcta aatcccgatc gagttggctg acgatcaaga cgatgaatcg atcatgtcgg
     2401 cagcatcttc ttccactgcc ggtctcgagc gcggcagcga caaaataaaa atgagcaaaa
     2461 ccaaaatctg cgaagcgaca caccagtagc agatggcctg cagcacgaac acctcgatat
     2521 aggtgagata caactgaaac atcacgccga tcaaggccac gccgaagatc aactgcgcgg
     2581 gccactgtcg gtctgcattg gtcggaataa agctcaagac caagatcgcc aggtaaccga
     2641 tcgagcccag caatgaaact ggaaacggac ccaggaacgc atagcggctg ctattcacaa
     2701 gttcgcagct gctggcgccg ccgcaaaaga tcgccacgcc aaacagatga gtataagtca
     2761 gataacccga cacacccaaa cccaacaacg ctagaatcgt gatcaaacgc tgccgcgtga
     2821 acatcgatcg atccttactt ggccgcattc aacagcgggc cgatatacaa ttcgaactgc
     2881 tggagcgata aagcgccgat gactttttgg ccgttgatga acaacgtcgg cgtctgagta
     2941 acgccgcgcg cgttggcatc ggccgtgtcg ctggtgacct tcgatcgata tttgccggta
     3001 ttcaaacagg ccgtaaattt atcggtgtcc aactgcagct gctgggcaaa gcccaccaga
     3061 tttgcatcgc taaacgcgcc gacattctcg ccgccttgat tggcaaacaa ggtgtcgtaa
     3121 tattcccaga acttgctttg ctcgccggca cattcagacg cctcaccggc cttgatcgat
     3181 tcctcaccga tgaaggcatc gtgatggaac acaaaacgca ctttgccact gctgacatat
     3241 ttgctttcaa tctgcggcag cgtctgccgt gcgaactggc cgcaggccgg acattgaaaa
     3301 tcgccatatt cttcaactaa gatcggcgca ttttgatcgc ccagcgaagt cccggcgacc
     3361 agcgcgtgct gctgcgcggc ctgtggtaga ctgaccagga caatcaaagc cacgcctgcc
     3421 atcgcggcga tgatgccgac gatccaccag gtagcggagg tattcttttt ctgaacgggc
     3481 cgtctggttg atttcatagg tactcattga tcctttcgtc atgaccctga ttgaaatatg
     3541 ctcgatcggg gtgaaattta ttctacacgg gccaccgccc accgtccggt gacggatgac
     3601 ccattgtaca acgacaggtg tcacttcacg ctcgcgcagg tcagggtaca cttattgtat
     3661 tcgcaaccat tgtccaattc aggagagcaa catggacatc gaacgtcaaa tcgagacgct
     3721 gcccaccaaa ttgggccaag cgattcaaaa aattgaagcg cctaagaaaa tttccttgtc
     3781 ggatgtgatc ctcggcggac aggatggatt ggtcaacgtt ttaggtgtga tcttgggggt
     3841 ggccgccgcg tccagcgatc cgcgcattgt gatcgccgcc ggactcgcgg cgacgtttgc
     3901 ggaatcaatc tcgatgggcg cggtagccta taccaccacc atggccgatc gggatttcta
     3961 cctgagcgag atcgaacgtg agaag
//
LOCUS       DASRTJ010000229         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_13357,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000229 DASRTJ010000000
VERSION     DASRTJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13357"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFF70_04815"
     CDS             <1..894
                     /locus_tag="VFF70_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44054.1"
                     /translation="PVANPKFTLTLDEQSSFIIQSFAMGLAAGASRIAVYKMADVPND
                     HAANPEPFGLVREDGSHRPAFAAFQVAANYLAGFTSATLDHRDDQYAVVSVKRGSAWT
                     TVAWTRQPLPVKITIPAHAAQALLIDANGRKQTITAKNGQYSFDIAGATCTGGCIIGG
                     SPRLIVEGSAGVSIAPPGPQSVKTLPSTSAVTSTTPSATIDLNGTIPATQPATSTPTS
                     TPTGTPTPTSTPASTPTTTSTATATAKSTSTPSPTPTPPLPVLSMSSDDNPFFVTIGI
                     LIGALVLGYVVVRVVSAGDRS"
     gene            957..2357
                     /locus_tag="VFF70_04820"
     CDS             957..2357
                     /locus_tag="VFF70_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY44055.1"
                     /translation="MATQVLPRSDNPQATLSIASPLIAPTTPTPDPTRAADTRQTELY
                     IVQSGDTLDSIALEYGATAQEVGQINGLIDLASIQPGDPLNVPLHLDRVGPSTKLLPD
                     SEFVYGPSTIDFDIKKFVDQTNGYLKSYTEELDGQTLTGAQIVQRVAEQYSVNPRLLL
                     AVIDYRGGWLSNATPDASSQYYPAGHHAETYAGLYQQLAWAANQLNDGYYGWRGRAMM
                     TVRFNDFSRARIGVGLNAGTAGAEEFLARDDNFDTWQKDIGSAGLMATYQKWFGDPFQ
                     YSTAIVSAKLIQPPLSLPWPIGEVWYYTGGPHGGWASGSGWAAIDFTPPDMVSCFPSE
                     YWVVAMSAGLVVRSEMGEVMVDLDGDGHEQTGWAILYMHIADQDRVSVGTKLNAGDHI
                     GHPSCTGGAANATHVHIARRYNGEWIPAGGSIPLILDGWKVTGEATEYDGGLVSDDQV
                     KVACECRDEAKNGIIR"
     gene            complement(2413..2907)
                     /locus_tag="VFF70_04825"
     CDS             complement(2413..2907)
                     /locus_tag="VFF70_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZY44056.1"
                     /translation="MRQENLTWHDVDTLIDHLLPQMRGSFDAMVMVTRGGLVPGGILA
                     EALDIKHVLTAAVRFPASEEKMLAWPTFLQFPEDELLRDRRVLVADDVWGSARTINTV
                     RGRVEAAGGRPETVVLHYKPSESLFKMKPDYYAAVTDAFIVYPWETNRRSMDVRLMEP
                     TMPM"
BASE COUNT          692 a          882 c          851 g          663 t
ORIGIN      
        1 ccggtggcga atccgaaatt taccctcacg ctcgacgagc aatcatcctt cattattcaa
       61 tcgtttgcga tgggcctcgc cgcaggcgca agtcgaattg cggtgtacaa aatggccgat
      121 gtgccaaacg atcacgcagc gaatcctgaa ccgttcggct tggtgcgaga agacggatcg
      181 catcgtcctg cgtttgcggc tttccaagtc gccgcaaatt atttagccgg tttcacctcg
      241 gccacacttg atcatcgcga cgatcaatat gcggtcgtct cagtgaaacg cggcagtgcg
      301 tggacaactg tcgcgtggac gcgtcaaccc ttacccgtca agatcactat tccggcgcat
      361 gctgcccagg ccctcttgat cgatgcgaat ggccgcaagc aaacgatcac ggcgaagaac
      421 ggacaatatt cgttcgatat tgcgggcgcg acctgcaccg gtggttgcat catcggcgga
      481 tcgccgcgct tgatcgtgga aggatcagcg ggtgtctcga tcgccccacc cggcccacag
      541 tctgtcaaga ccttgccatc cacaagtgct gttacatcga ctacgccaag tgcaacgatc
      601 gatctgaatg gaacgatacc cgccacacag ccagcgacat caactcccac atcgacgccg
      661 actggcacgc caactccaac ttcgacgccg gcatcaacac caaccacgac ctcaacggcc
      721 actgcgaccg ctaagtcaac ttctacgcca tcgccgacac cgactccacc tttgcccgta
      781 ttgagtatgt catcagacga taatccgttc ttcgtcacga ttggaatttt gatcggtgca
      841 ctcgtcttgg gctatgtcgt tgtgcgcgtc gtatccgccg gagatcgttc gtgattcgat
      901 cccagacatc gctccttctc gcgttcatgc taattctgcc cgcgtgctcg cgtccagtgg
      961 caacgcaagt ccttccacgc tctgataacc cgcaagcgac gttatccatt gcatccccat
     1021 tgattgcgcc gaccacgccc acgcccgatc ccacgcgtgc cgctgatacg cgccaaacgg
     1081 agttgtacat tgtgcaaagc ggtgacacgt tagatagcat cgcgttggaa tacggcgcaa
     1141 ccgcgcaaga agttggacag atcaacgggc tgatcgatct cgcgtcgatc caaccgggcg
     1201 atccgctcaa cgtgccgctg catctcgatc gggttgggcc aagcaccaaa ttgttacccg
     1261 acagcgaatt cgtttatggt ccgagcacga tcgattttga tataaagaag tttgtcgatc
     1321 agaccaatgg ctatttgaaa tcgtacacgg aagaactcga tggacaaacg ctgaccggcg
     1381 cgcagatcgt tcagcgcgtg gccgaacaat acagtgtgaa tccgcgtttg ctcttggccg
     1441 tgatcgatta tcgcggcggt tggttgagca acgccacgcc cgatgcctcg tcacaatatt
     1501 atccggcggg tcatcacgcc gaaacctatg cgggtttgta tcagcaatta gcctgggcag
     1561 cgaatcagtt gaatgacggt tactatggct ggcgcggacg cgccatgatg acagtgcgct
     1621 tcaacgattt ctcgcgggcg cgaatcggcg tggggctaaa tgccgggacc gctggggccg
     1681 aagaattttt ggcgcgggat gataactttg atacttggca aaaagatatt ggctcagctg
     1741 gattgatggc gacatatcag aaatggttcg gcgatccatt ccaatactcg accgcgatcg
     1801 tttctgcaaa attgatccaa ccgccgttgt cgctgccatg gccaatcggg gaagtgtggt
     1861 actacacggg cgggccgcac ggcggctggg ccagcggatc gggctgggca gcgatcgatt
     1921 tcacaccacc cgatatggtg agttgttttc catcagaata ctgggtcgtg gcgatgtcag
     1981 cgggcttggt ggttcgatca gaaatgggcg aagtgatggt cgatctcgat ggcgacgggc
     2041 acgagcaaac gggctgggcg atcttatata tgcacatcgc cgatcaagat cgcgtgtccg
     2101 ttggaacaaa attaaacgca ggcgatcaca tcggtcatcc gagttgcaca ggcggcgcgg
     2161 ccaatgcgac gcatgtccac atcgcacgca gatataacgg cgaatggatt ccagccggcg
     2221 gatcgatccc gctgatctta gatggctgga aagttacggg tgaagcaaca gaatacgatg
     2281 gcggattagt gagcgacgat caagtgaagg tcgcgtgtga atgccgcgat gaggcgaaga
     2341 atggaataat acggtgaggg cgaggctata gtccccgaag cagagcggag cgggatcacc
     2401 tcacccttac aattacatcg gcatcgttgg ttccatgagc cgcacgtcca tcgatcggcg
     2461 attcgtttcc cacggataga cgatgaaggc atccgtcacg gccgcgtaat aatcgggttt
     2521 cattttgaaa agcgattcgc tcggcttgta atgcagcaca acggtctccg gtcgaccgcc
     2581 cgccgcttcg acgcggccgc gcacggtatt gatcgtgcgc gcgctgcccc acacatcatc
     2641 cgccacgagc acccgtcgat cgcgcagcag ttcatcttcg gggaattgca ggaaagtcgg
     2701 ccaggccaac attttttctt cactggccgg aaatcgaacg gccgcggtca ggacatgctt
     2761 gatgtccaac gcttcggcga ggatgccgcc cggcaccaag ccgccgcgtg tgaccattac
     2821 catcgcatcg aacgagccgc gcatttgcgg caacagatga tcgatgagtg tatccacatc
     2881 atgccacgtc aaattttctt ggcgcatgtt agtcctccgc agctccgtta atgaagcgga
     2941 ttatatcata cggttatcac gtgtggcgga tcgattgtat tgcgtggtgc gtattgcgca
     3001 gacattctga tccctgatcc ccgatccacg aatccctgct ttagcgtccc tgccaccaat
     3061 ctttgcgcga acgcggatcg aggaaaga
//
LOCUS       DASRTJ010000230         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_270802,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000230 DASRTJ010000000
VERSION     DASRTJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270802"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFF70_04830"
     CDS             <1..781
                     /locus_tag="VFF70_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZY44057.1"
                     /translation="HYWAAQTLVIVAVLHMLRVIFTGAYKAPRKTNYVIGILLLAFIV
                     LFNFTGYVLRWDEGTNWALTVGTSLLKEIPVIGNAVYQIIVGGSDIGSATTIRFYAWH
                     VIGLTLIVVILIAWHAFKVRRDGGISHRSREPRIDRARLVTMETKAMIVVTALLILIS
                     IVLPAPIGPVADLPTRSGSVQAPWFFLWIQFLLRSLPAVWAGVLIPIGILAILILIPY
                     LIDRKLDGVGEWFNRSGRIAQVISILIIAGILLLSIIELGA"
     gene            863..2170
                     /locus_tag="VFF70_04835"
     CDS             863..2170
                     /locus_tag="VFF70_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44058.1"
                     /translation="MKIDRFFAFGSILLTSLMTLAIIRQLSLPAPRSITPTLTGKQEL
                     CLTCHYGIEEISKSHPVEAFGCVSCHGGDPLALDKQVAHTGLRGGKNPADFSAVEASC
                     GGSQCHSGSIADNNDHIQRSTTSIQATYAGGIVQVRRAFGAQSDAVARFGIDAITSEV
                     TPTDRAVTQLDRFDPVASNDPQAVIEFGQRCLNCHLSSEPIMQPYAYRSTGCAACHVT
                     YASDGLYRGGDPTIDPAQPGHASLHQLTTKISYTTCNTCHNRGNYSLKQMTFLPRNDL
                     PTSGSPLPEDRLRDYYQPIAQFTKCEWELGCIDCHTSKQVMGDGQLYSSKLDIQHTQC
                     KTCHGTLTELPQTTTITDPNDVELRRAQLNGNYSLQVGDKVIAIRADEKFGAIKLING
                     QYIETMKVSGQSLVIPLATGSLCKQRPDQQSSNYCHECHAVKR"
     gene            2250..>2463
                     /locus_tag="VFF70_04840"
     CDS             2250..>2463
                     /locus_tag="VFF70_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZY44059.1"
                     /translation="MLDESALIESLRRCEPDSFTRLFEAYSDRIYRLAVRFLNDTSES
                     EEIVQDTFLTICEKIDSFQGRSKLGTW"
BASE COUNT          565 a          660 c          643 g          595 t
ORIGIN      
        1 gcactattgg gccgcgcaaa cattggtcat cgtcgcggta ctgcatatgc tgcgcgtgat
       61 cttcaccggc gcatacaaag cgccgcgcaa aacaaattac gttatcggca ttttgttgct
      121 ggccttcatc gtcctattca atttcaccgg ctacgttttg cgctgggatg aaggcacgaa
      181 ttgggcgttg acggtcggca ccagtctgct aaaagaaatc cccgtgattg gcaacgcggt
      241 gtatcagatc atcgtcggcg gatcagatat aggctcggcg acaacgatcc gcttttacgc
      301 gtggcacgtc atcggcctca ccttgatcgt tgtgattttg atcgcgtggc atgccttcaa
      361 agtccggcgc gatggcggaa tctcacatcg atcgcgtgag ccgcgtatcg atcgagcgcg
      421 cctggtcacg atggaaacaa aggcgatgat cgtcgtgacc gcgttgttga tcttaatctc
      481 gatcgttctc ccggccccga tcggtccggt tgccgatctt cccactcgat ccggcagcgt
      541 gcaagcgccg tggttcttct tgtggattca gtttctcttg cgatcattgc ccgctgtgtg
      601 ggcgggtgtg ctgatcccga tcggcatcct ggcgatcttg atcttgatcc catatctgat
      661 cgatcgcaaa ctcgacggtg tcggtgagtg gttcaatcga tccggccgaa tcgcacaggt
      721 gatttcgatc ctgatcatcg caggcatatt gttactgtct atcatcgaat taggcgcgta
      781 agatcaaatc acagagatgc agaggcacag agtcttgttt aattttctct gcgccttggt
      841 gactctgtgg ttttaatttg ctatgaaaat cgatcgattc tttgcatttg gctccatcct
      901 gttaacctcg ctcatgacgc tggcgatcat tcgccaactc agcctgcctg cgccgcgatc
      961 gatcacgccg acgttgaccg gcaaacaaga gttgtgtctc acctgccact atggcatcga
     1021 agagatcagc aagtcgcatc cggtcgaagc gtttggctgc gtcagttgtc acggcggcga
     1081 tccccttgcg ctcgataaac aggtcgcgca taccggactg cgcggcggca aaaatccagc
     1141 ggacttcagc gccgtcgaag catcctgcgg tggatcacag tgccactccg gatcgatcgc
     1201 ggataacaac gatcacattc aacgctcgac gacgagcatt caagcgacat acgccggcgg
     1261 aatcgtgcaa gtccgtcgag cgtttggtgc acaatcggat gccgtggcac gttttggaat
     1321 cgatgcaatt acgagtgaag tcacaccaac tgatcgggct gtcacccaac tcgatcgatt
     1381 tgatccagtt gcctcaaatg atccgcaggc cgtgatcgag ttcgggcaaa ggtgtctcaa
     1441 ctgtcacctc tcttcagaac cgatcatgca accgtacgct tatcgatcga ctggttgtgc
     1501 agcgtgtcat gttacttacg ccagcgatgg cttatatcgc ggcggcgatc cgacgatcga
     1561 tcccgcgcag cccggtcacg cctcactgca tcaattgaca acaaagattt cttatacgac
     1621 gtgcaacacc tgccacaatc gcggcaatta ttcattaaag caaatgacgt ttctcccacg
     1681 gaatgatcta ccgaccagcg gatcgcccct gcctgaagat cgattacgcg attactacca
     1741 accgatcgcg cagttcacca aatgcgaatg ggaacttggc tgcatcgact gtcacacctc
     1801 gaagcaggtc atgggcgacg gccagttgta cagcagcaaa ctcgatattc agcacacgca
     1861 gtgcaaaact tgccacggca ctttaaccga gttgccgcaa acgacgacca tcaccgatcc
     1921 gaacgatgtc gaactgcggc gcgcacagtt gaatggaaat tactcgttgc aagtcggcga
     1981 caaagtgatt gcgattaggg cggacgaaaa attcggcgcg atcaagttga ttaacggcca
     2041 atacatcgaa actatgaaag tcagcgggca gtcactggtt attccgttgg cgaccggctc
     2101 actctgcaaa cagcggcctg accaacaatc atcgaattat tgtcatgagt gccacgcggt
     2161 taaaagatag aggctggaag gtggatgttc gaagttggaa tttcgtgtaa ccttttgaat
     2221 cattttgcat ctaaagatca gaggctttca tgctggatga gtctgcctta atcgagagtc
     2281 tgcggcgctg cgagccggat tctttcacgc gcttatttga agcgtattct gatcgaattt
     2341 accggctggc cgtgcgtttc ttaaacgata cgagtgaatc tgaagagatc gtacaggata
     2401 ccttcctgac gatctgcgag aagatcgatt cctttcaagg ccgatcgaag ttgggcacgt
     2461 gga
//
LOCUS       DASRTJ010000231         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_316395,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000231 DASRTJ010000000
VERSION     DASRTJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316395"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFF70_04845"
     CDS             complement(<1..250)
                     /locus_tag="VFF70_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44060.1"
                     /translation="MPDDYEFEEEEFTTQFNGQTLRRILAQTRPHWKWVAGFLIAVGL
                     VVLLDSIFTYLRKQMLDQGITAGDQEALTQIMTIYGLMI"
     gene            complement(345..2174)
                     /locus_tag="VFF70_04850"
     CDS             complement(345..2174)
                     /locus_tag="VFF70_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44061.1"
                     /translation="MSALSAPTPFDLKATVSESRLKGLWRMISGYRLAYVGALASLAV
                     AATSKTLTYLLLAYLIDDVLGKGIFDNTLLLIGLGFVGLALIEGLFTFFSGRLAALTS
                     EGIAQRLRDYLFDHIQRLSFTYHDKAQTGELIQRSTSDVDAIRRFFADQAINAGRIVL
                     LFLINFIALLRLNSSLALFSVIVIPIVLLISIWFFQKISKAYEAHQEQEAALSTTLQE
                     NLTGVRVVKAFARQAYERNKFETVNWEKYKRGRKLLTIHALYWPITDILCGFQMLAGF
                     IIGALMTMNGTLTLGEYVAYAGLVIWLIWPMRNLGRLIVQMSTGLVSYGRVVEVIKEV
                     REPLTEGSIKPQGSVRGEIVFKHVSFQYEKDSPVLNDISFRCEPGQAIALLGSTGSGK
                     TTLVNLLPRFYEYSGSLTLDGVELNHYPRKYLRTQIGIVEQESFLFSRTIRENITYSV
                     GRDVPQTEIEEAARAAAIHDVIMTFPDGYNTLVGEKGVTLSGGQKQRVAIARTLLKNP
                     RILILDDSTSSVDLETEEEIRDALQTLMKDRTTFIIAHRIQSVMNADLILVMDHGRIV
                     QSGQHEELIGQPGIYRRIYDIQMRIEVELEQEVNQAQAAELNQ"
     gene            2424..>2711
                     /locus_tag="VFF70_04855"
     CDS             2424..>2711
                     /locus_tag="VFF70_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44062.1"
                     /translation="MSAQPPSDQSTPNSKSLPRKLIDAYVRYQKEFTLQGELKGFYLA
                     RRLWTVSSLSRLGLIALLIGLWLTTTAFSTQQSTTATLTGLAMFALGWAFAL"
BASE COUNT          640 a          776 c          686 g          609 t
ORIGIN      
        1 ggatcatcaa accgtagatc gtcatgatct gcgtcaacgc ctcttgatcg cctgccgtga
       61 taccttgatc gagcatctgc ttgcgcaggt aggtgaagat cgaatcgagc agcacgacca
      121 gccccacggc gatcagaaaa ccggccaccc atttccagtg cggccgggtc tgcgccagaa
      181 tccgccggag cgtctggccg ttgaactgcg tcgtaaattc ttcttcttca aactcgtaat
      241 cgtctggcat gttaaatcca ttcgtagggg caagccttgt gctcgcccag ggcaaccacg
      301 agggttgccc ctacgcatca atccaattct ttttcattcg tggatcactg attcaattcc
      361 gctgcttgcg cttgattgac ttcctgctcc aactccactt caatccgcat ctggatgtca
      421 tagatccggc gatagatgcc gggctgcccg atcagttctt catgctgacc ggactgaaca
      481 atgcggccat gatccatgac caggatcaga tcggcattca tgacgctttg aatgcgatgc
      541 gcgatgatga acgtggtgcg atctttcatc agcgtttgca gcgcatcgcg gatctcttct
      601 tccgtctcca gatcgaccga cgaggtggaa tcgtccagga tcaagatgcg cggattcttc
      661 aataacgtgc gcgcgatcgc gacgcgctgt ttctgcccgc ccgataacgt cacgcccttt
      721 tcgccgacga gcgtgttgta accgtcgggg aaagtcatga tcacgtcgtg aatcgccgcc
      781 gcgcgcgccg cttcttcgat ttcggtttgc ggcacatcgc gtccgacgct gtaggtgata
      841 ttctcgcgga tcgttcttga aaagagaaac gattcctgct cgacgatgcc gatctgtgtg
      901 cgcagatatt tgcgcggata atgattcagc tcaaccccat cgagcgtgag gctgccggag
      961 tactcgtaga agcgcggcag caaattcacc aacgtcgtct tgccggagcc ggtcgagccg
     1021 agcagcgcga tcgcctgacc gggctcgcat cggaaagaaa tatcattcag caccggacta
     1081 tctttttcat attgaaaaga cacatgcttg aagacaatct cgcctcgcac gctgccctgc
     1141 ggcttgatcg atccttcagt caatggctcg cgcacttctt tgatcacttc gaccacgcgg
     1201 ccgtatgaca ccaagccggt ggacatctgc acgatcaagc ggccgaggtt gcgcatcggc
     1261 cagatcaacc agatcaccag accggcgtaa gccacatact cgcccagcgt cagcgtgcca
     1321 ttcatcgtca tcaatgcgcc aatgataaag ccggccagca tctggaagcc gcacagaata
     1381 tcggtgatcg gccagtacag cgcatggatc gtcaacagct ttcgaccgcg tttgtacttt
     1441 tcccaattga cggtctcaaa tttgttgcgc tcatatgctt gccgcgcaaa agccttgacc
     1501 acgcgcactc cagtcaagtt ttcctgcagc gtggtcgaga gcgccgcttc ctgttcttga
     1561 tgcgcttcgt acgctttcga aattttctgg aagaaccaga tggatatcag cagcacgatc
     1621 gggatgacga tgaccgagaa taaagctaac gaactattca aacgcagcag cgcgatgaag
     1681 ttgatcaaga acaacagcac gatccgtccc gcgttgatcg cttgatcggc gaagaagcgg
     1741 cggatcgcat cgacatccga agtcgatcgc tggatcaact cgcccgtctg cgctttatcg
     1801 tgatacgtga aggacaaacg ctgaatgtga tcgaacaaat aatcgcgcag gcgctgcgca
     1861 atgccctcgg aggtaagcgc cgccaagcgt ccgctaaaaa acgtaaacag cccctcgatc
     1921 aacgccaagc cgacaaaacc cagcccgatg agcagcagcg tgttgtcgaa gatgcctttg
     1981 cccagcacat cgtcgatcag ataagccaac agtaagtagg tcaacgtctt ggacgtcgcc
     2041 gcaacggcca gactggccag cgcgccgaca tacgccaggc gatagccgct gatcatgcgc
     2101 cacagtcctt taaggcggct ctcggaaacg gtggctttca gatcaaatgg ggtaggtgca
     2161 gaaagtgcgg acaaaataat caatctccga tgtaaaatcc aaagaagatt ctaccacgat
     2221 catgttgctc aatcgttggc gtcgtgttat cctaatctac atccatcgga ttttcgcttg
     2281 tcatttcgaa cgcagtgaga aatctctgct ccaatgagca aaccatgccg atgatcgttg
     2341 agatttctcg aaaaaaacgc ttgaaatgac cgctaagcgt atgtcctgat tcgtttactc
     2401 acagcaacgg tgcgagcaat cccatgtcag cgcaaccgcc ctccgatcaa tcgacgccaa
     2461 attccaaaag tcttccacgc aaactgatcg acgcgtatgt gcgttatcaa aaagaattca
     2521 cgctgcaagg cgaactcaaa ggtttctatc tggctcgacg tctctggacg gtcagcagct
     2581 taagccgcct gggactgatc gcgttgttga tcggtttatg gttgacgaca acggctttct
     2641 ccacgcagca atcaacaacg gcgacgctca ccggtttagc gatgttcgcg ttgggctggg
     2701 cgtttgcact c
//
LOCUS       DASRTJ010000232         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_51668,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000232 DASRTJ010000000
VERSION     DASRTJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51668"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VFF70_04860"
     CDS             complement(<1..633)
                     /locus_tag="VFF70_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY44063.1"
                     /translation="MKLAISGKGGVGKTTLAALLAQAYADLGRNVLAVDADPSPCLAG
                     ALGFPPELRSKLHPIAEMDQLIEERTGAKPGTVGGFFTINPRVDDIPDRFSIAHRNVR
                     LLEMGSVDIGGSGCICPESALLKTLFTHLLFRQDDVLILDMYAGVEHLGRATVDFVDA
                     MIIVVEPTRRSLGTAGQIKKLANDIGLTRLWLVGNKVRNQDEVDFLKTETP"
     gene            complement(617..847)
                     /locus_tag="VFF70_04865"
     CDS             complement(617..847)
                     /locus_tag="VFF70_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44064.1"
                     /translation="MIGDQDITFGMLVQLTLLNDAEVLTSRTLWSDEVLQAAQRACTS
                     GLNLSAVMLALRAEAQRRDQIDTKKDQHEVSD"
     gene            complement(1072..2367)
                     /locus_tag="VFF70_04870"
     CDS             complement(1072..2367)
                     /locus_tag="VFF70_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44065.1"
                     /translation="MKDYLRDRLRISEQHLNDINALLLDPKTTVVNEFLEVVRKYGTP
                     EEINRKADEARQLPNLMARLKKINSPYLHDLDWLIVERNQGAFISEADYRREVLGPKA
                     DQLSFADDFAVTLEISAFQYFPWLIAEAKQAIAKQELMPGRFIRVRKMKESEADKGDM
                     LAVAAAMQIIGASYVETLDTKGTDGSNVHLGGPATITGYFGGVGEPNEYPLKWLDEFL
                     YYYTTYGTQQVLNLNPGTVLLGYLLHKLGVNNEFKISVFMGNDNPYAVLWTLIGARLF
                     ARPDGTSPLIGFNFSNSVNNQTIELCAEMRGALDFEKVVRFEHHITETWKSIVRQPYD
                     RRDELIEIAGHIANIGAKHEGGDPEIEEKLDHPSDILDYFRDQQEVIESGDMPKLELN
                     YLEKHNALNHTARALTEHGLSFVAARHLHHEPAREPVPA"
     gene            complement(2416..3291)
                     /gene="panB"
                     /locus_tag="VFF70_04875"
     CDS             complement(2416..3291)
                     /gene="panB"
                     /locus_tag="VFF70_04875"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZY44066.1"
                     /translation="MTDRKKITLPMLQKKMLEGVPITMITCYDYAMAYLVEQAEIDMV
                     LVGDSLGMTMLGYEGTLPVTMDDMMRHTAAVRRGSSTSWLIGDMPYMSYQPSDEIAVK
                     NAGRFMAETGCDSVKLEGGRQMASRVKAIADAGIPVMGHLGLTPQSVSALGGFKVQGK
                     GADQAARIIEDAKILEEAGAFSILFEMIPDRVQKVITERAHIPIISLGSGPDAHGQLL
                     IFHDMFGLYPRFTPKMAKVFGNAGEVILNGLKQYVAEVTAKQFPQPENWFGIKDDQFE
                     ELQHKFNGQQREPVG"
     gene            complement(3321..>3589)
                     /locus_tag="VFF70_04880"
     CDS             complement(3321..>3589)
                     /locus_tag="VFF70_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44067.1"
                     /translation="TVPDRLLKRLEAAGDRADEEGVQIALELIDQIKTKQGVHGIHIM
                     AIGWEDIVPRIVKEAGLLPAGFVEPPAPQTPAKEKKTISEPIAV"
BASE COUNT          759 a         1056 c          929 g          845 t
ORIGIN      
        1 gggcgtctca gttttgagaa aatcaacttc gtcttgattg cgaactttgt tgccgacaag
       61 ccacaagcga gtcaagccaa tatcattcgc taattttttg atctgcccgg ctgtgcccaa
      121 acttcgccgc gtcggttcaa ccacaatgat cattgcatca acaaagtcga ccgtcgcgcg
      181 gccaagatgt tcaacgcccg catacatatc gaggatcaac acatcatctt ggcggaagag
      241 caagtgagta aacaaagtct tcagcagcgc gctctcagga cagatacagc ccgatccgcc
      301 gatgtcgacc gatcccattt cgagcaagcg tacattgcgg tgggcgatgc tgaatcgatc
      361 ggggatgtcg tcaacacgcg gattgatcgt aaagaagccg ccgacggtgc cgggcttcgc
      421 acccgtccgt tcctcgatta attgatccat ctccgcgatc ggatgcagct tcgatcggag
      481 ttcaggcgga aaacccaatg cgccggctag acacggcgac ggatcggcat cgaccgccag
      541 aacgtttcga cccagatcgg catacgcttg cgcgagtagc gccgctaagg ttgttttccc
      601 gacaccgccc ttgccactaa tcgctaactt catgctgatc ctttttagtg tcgatttgat
      661 ctcgccgctg cgcttcagcg cgcagagcca acatcactgc gctaagattc aagccgctgg
      721 tgcacgcccg ctgtgccgcc tgcaagactt catctgacca caacgttcga ctggtcagga
      781 cttcagcgtc attcagcaaa gtcagttgaa ctaacatgcc gaatgtaata tcttgatccc
      841 caatcatcgc ctcatcacat agccagcaat ttttgcagcg ccgaacatct tggccgcaag
      901 ctgcgagcac gctagtgcgc aaggtgggtc gaagaaaatc aacctgagaa tcggacataa
      961 cggtctgcat gattatgctt ctattttaag caaaacgcgg ggcgaatact aatgatgttc
     1021 gtcaatcaaa catcagacat tcgtcccgcg ttaattcaac tttccgctgg attaggctgg
     1081 caccggctcc cgcgcaggct catgatgtaa atgccgcgcc gcgacaaaac tcaagccgtg
     1141 ctcggttaaa gcgcgggccg tgtgattgag cgcgttgtgc ttttccagat agttgagctc
     1201 caattttggc atgtccccgc tctcgatcac ttcttgttga tcgcggaaat aatcaaggat
     1261 gtcggaggga tgatcgagtt tctcttcgat ctccggatcg ccgccttcgt gcttggcgcc
     1321 gatgttggcg atgtgcccgg cgatctcgat cagttcatca cgccgatcgt acggctgccg
     1381 cacgatcgat ttccacgtct cggtgatgtg atgctcgaag cgcacgacct tttcaaagtc
     1441 gagcgcaccg cgcatttcgg cgcacaactc gatcgtttgg ttgttgaccg agttgctgaa
     1501 gttgaaaccg atcagcggcg aagtcccatc gggccgggca aacagccgcg caccgatcaa
     1561 cgtccacagc acggcgtagg gattatcatt gcccatgaac accgaaatct tgaactcgtt
     1621 gttcacgccg agtttatgca gcagatagcc gagaagcacc gtgcccggat tgaggttgag
     1681 cacttgctgg gtgccatacg tcgtgtagta atacagaaat tcgtcgagcc acttgagcgg
     1741 atactcgttc ggctcaccta caccgccgaa gtaaccagtg atcgtggcgg gtccgccgag
     1801 atgcacgttc gatccgtccg tgcctttggt gtccagcgtt tccacgtaac tcgcgccgat
     1861 gatctgcatc gcggcggcga cagccagcat gtcaccctta tccgcttccg attctttcat
     1921 cttgcgcaca cgaatgaagc ggccgggcat caattcttgc ttggcgattg cttgtttcgc
     1981 ttcggcgatc agccacggaa aatattggaa agcgctgatt tccagcgtca cggcgaaatc
     2041 atcggcgaag gacagctgat cggctttagg gccgagcact tcgcgtcgat agtcggcttc
     2101 gctgatgaat gcaccttggt tgcgctcgac aatcagccaa tcgagatcgt gcagatacgg
     2161 cgagttgatc ttcttcaagc gcgccatcag attaggcagc tgccgtgcct catccgcttt
     2221 ccgattgatc tcttcgggcg tgccatactt gcgcacgacc tcgaggaatt cgttgaccac
     2281 cgtggtcttc ggatcgagca acagggcatt gatgtcgttg agatgctgct cggaaatgcg
     2341 cagtcgatcc cgcagataat ctttcatggt gaaacctcca agtcgctatg gactttcaag
     2401 ggttgcccga atcgattacc cgaccggttc gcgctgctgc ccattgaatt tgtgctgcaa
     2461 ttcttcaaat tgatcgtctt tgatgccgaa ccaattctcc ggctgcggga attgtttggc
     2521 agtcacttcg gccacatact gcttgaggcc gttcaagatc acttcgcccg cattgccgaa
     2581 aaccttcgcc atcttcggcg tgaagcgcgg atacaggccg aacatatcat ggaagatcaa
     2641 caattgaccg tgcgcgtcgg gcccggagcc gagactgatg atcgggatat gggcgcgctc
     2701 ggtaatgacc ttctgcacgc gatcggggat catctcaaat aagatcgaga acgcgccggc
     2761 ctcttccaga atcttcgcat cctcgatgat ccgtgcggcc tgatcagccc ctttgccttg
     2821 caccttaaat ccgcctaacg ccgagacact ttgtggtgtc agtccgaggt ggcccatcac
     2881 cggtatgccc gcatcggcga tcgctttgac gcgcgaggcc atctgccgcc cgccttccag
     2941 cttcaccgaa tcgcagccgg tctcggccat gaaacggccc gcgttcttca ccgcgatttc
     3001 atcggaaggc tgatagctca tgtacggcat gtcgccgatc agccaacttg ttgacgagcc
     3061 gcgccgcacg gcggccgtat gacgcatcat atcgtccatc gttaccggca gggtaccttc
     3121 gtagccgagc atcgtcatgc ccagactgtc gcccactagc accatatcga tctccgcctg
     3181 ctcgacgaga tacgccatcg cgtaatcgta gcaagtgatc atcgtaatgg gaaccccctc
     3241 cagcattttc ttttgcaaca tcggcaatgt aattttcttt cggtcggtca tggtcgctta
     3301 ctcctaagtg tgtccgtctg ctaaactgcg atcggctcac tgatcgtctt cttctctttt
     3361 gccggagttt gcggcgcggg tggttcgaca aagcccgctg gcaacagtcc agcctctttg
     3421 acgatgcgcg gcacgatgtc ttcccagccg atcgccatga tgtgaatccc gtgaacgccc
     3481 tgcttcgttt tgatctgatc gatcaactcc agcgcgatct gcacgccttc ctcatcagcc
     3541 cgatcgcccg cggcctctaa gcgcttgagt aaccgatcgg gcacggtga
//
LOCUS       DASRTJ010000233         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_88979,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000233 DASRTJ010000000
VERSION     DASRTJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88979"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFF70_04885"
     CDS             complement(<1..238)
                     /locus_tag="VFF70_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44068.1"
                     /translation="MYSQSRIIRLSLATTLGVVVASLCLLSLSAQPSGAAHADTPPRQ
                     AADLAPVVIHPQGFGETKPLRDLPPAPQTLPAQAT"
     gene            complement(331..789)
                     /locus_tag="VFF70_04890"
     CDS             complement(331..789)
                     /locus_tag="VFF70_04890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44069.1"
                     /translation="MAIDLRRARSTEADQLTTIAIAAKRQWGYPDRWIDLWREQLTIA
                     ADYIAAHEVYAATIDQVIVGFYAVSDSGSISILDHLWVQPNSIGRGVGRRLLQHAIDQ
                     AKRSGAHSIEIESDPNAEGFYQHMGARRVSVKRHELEGQARELPVLRIDL"
     gene            830..1306
                     /locus_tag="VFF70_04895"
     CDS             830..1306
                     /locus_tag="VFF70_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZY44070.1"
                     /translation="MQIAKNTVVTIDYTLTDDQGSVLDTSKGAVPLVYMQGSGNLIPG
                     LERALEGKSTGDQFKVSIPPEEAYGLRVDENMEDVPLSAFPGDEELEVGSQVEVQHDH
                     GTHVMTVVAINDDSITLDANHPLAGEILNFDVRVVAVREAAAEELEHGHAHGGHGH"
     gene            complement(1378..>1560)
                     /locus_tag="VFF70_04900"
     CDS             complement(1378..>1560)
                     /locus_tag="VFF70_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY44071.1"
                     /translation="ALALAYLGIAIALDALCAIALWPIMLIALTDVVIVREEKYLEAK
                     FGDEYTQYKSQVRRWI"
BASE COUNT          367 a          400 c          451 g          342 t
ORIGIN      
        1 cagtggcttg cgcgggcagg gtctgtggag caggcggcag atcacgcagt ggtttcgtct
       61 cgccaaagcc ttgcgggtga atgaccaccg gagccaagtc cgcagcttgg cgaggtggcg
      121 tgtcggcgtg tgccgcacca ctaggttgcg ccgataaaga taataaacat agactggcta
      181 caacaacgcc aagcgtggtc gctagtgata agcgaatgat gcgagattgc gagtacatgt
      241 tgcacacacc tttctgttgt atcagttgaa tggggaggac agtggctgtg gcaacagtat
      301 agcatggatt cagcagcgca accgattttg ttagagatca attctcagca ccggcagttc
      361 acgcgcttgc ccttcgagtt catgtcgttt cacactcacg cgccgcgcgc ccatgtgttg
      421 ataaaaacct tcggcgttgg gatcagattc aatctcgatc gaatgtgcgc ccgatcgttt
      481 agcttgatcg atcgcgtgtt gtaacaaacg tcggcccaca ccgcgcccga tcgagttggg
      541 ctgaacccac aggtgatcga ggatcgagat cgatcccgaa tcgctcaccg catagaagcc
      601 gacgattact tgatcgatag tagccgcata aacttcatgc gcagcgatgt agtcagcagc
      661 gatcgtcaac tgctcgcgcc aaagatcgat ccaccgatcg ggataacccc actgccgttt
      721 ggccgcaatc gcgatcgtcg ttaattgatc cgcttcggtt gatcgcgcgc ggcgcaggtc
      781 gatcgccata tgttataatc gccaaacttt tatggactgg agacacgcga tgcagattgc
      841 caagaacacc gtcgtcacca tcgattatac tctcaccgac gatcaaggct cggtgctgga
      901 tacttccaaa ggcgcggtgc cgttggtgta tatgcagggc agcggcaatt tgatccccgg
      961 cctggaaaga gcgttggaag gaaaatcgac gggcgatcaa ttcaaagttt cgattccgcc
     1021 cgaagaagcg tacggcctgc gcgttgacga gaacatggaa gacgtgccgc ttagcgcatt
     1081 tccaggcgat gaagaattgg aagtgggcag ccaagtggaa gtgcagcacg atcatggcac
     1141 ccacgtcatg acggtcgtgg cgatcaatga cgattcgatc acgctcgatg ccaatcatcc
     1201 gttggcgggc gagatattga atttcgatgt gagggtggtg gctgtgcgtg aggcagccgc
     1261 tgaagaattg gaacacgggc acgcgcacgg cgggcacggg cattagcccg atcgattttt
     1321 caggcgaatg aaattaaacg aacggcggac aatttgtccg ccgtttttag tgctgtatta
     1381 aatccaccgt cgcacttgcg atttgtactg cgtgtactca tccccgaatt ttgcttcgag
     1441 atatttttct tcgcgcacaa taacgacgtc ggttagggca atcagcatga tcggccacaa
     1501 ggcgatggca catagggcat ccaacgcgat ggcgatgccg agataggcta aggccagcgc
//
LOCUS       DASRTJ010000234         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_119334,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000234 DASRTJ010000000
VERSION     DASRTJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119334"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VFF70_04905"
     CDS             complement(<1..85)
                     /locus_tag="VFF70_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HZY44072.1"
                     /translation="MTQFIFFSGKGGVGKTSLACTQAVRYAD"
     gene            complement(82..441)
                     /gene="arsD"
                     /locus_tag="VFF70_04910"
     CDS             complement(82..441)
                     /gene="arsD"
                     /locus_tag="VFF70_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HZY44073.1"
                     /translation="MSDTISLFDAAPIHPQAAVVELFDPPMCCPTGLCGPTLDQTLLD
                     VSEMIVALQTDGLNVERYQMTSHPQAFLSNPDVMRLVREKQMAALPITVVRGLVVKVG
                     AYPTLTEIKSYLDGATA"
     gene            complement(479..742)
                     /locus_tag="VFF70_04915"
     CDS             complement(479..742)
                     /locus_tag="VFF70_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZY44074.1"
                     /translation="MLTIKVLGPGCSNCKRMEQLAQLVVKELDVDAYIEKITDYAEIM
                     RYPILSTPGLVINEQLVCSGRIPNRAEVTTWITTALEHQPQGA"
     gene            complement(761..1141)
                     /locus_tag="VFF70_04920"
     CDS             complement(761..1141)
                     /locus_tag="VFF70_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZY44075.1"
                     /translation="MNEFDRSAQLFKVLMHPARLEILDVLRNGEACVCHIEAALSYRQ
                     AYISQQLAALREAGLIQDRREGWNIYYRVTRPQVFEIIDAALRLNGTHRLSRVKSMKS
                     ASLDNCPCPKCNPTVELKVAKIQI"
     gene            1445..2608
                     /gene="pstS"
                     /locus_tag="VFF70_04925"
     CDS             1445..2608
                     /gene="pstS"
                     /locus_tag="VFF70_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZY44076.1"
                     /translation="MQRNTRFVAITLLIAVLVSACAPAATPAAPAATQPPVVQTVVVQ
                     PTAGPAQPTAVPAGSITINGAGATFPFPLYSKWFYDYAFVDTSVKFNYQSIGSGGGIQ
                     QITAKTVDFGASDSILNDAQYTAAPGIQMFPTVAGAEAIVVNLKGDDGNPITTTLNFP
                     YTSVADIFAGKITKWNDPVLTAANPNVTLPNKDIIVVHRSDGSGTTFIFTSYLSSVSA
                     DWKTNVGAASSVKWPVGLGGKGNEGVFGTVSQNDGAIGYVELAYALQNKATINALQNK
                     AGEFVKPSIASTQAAMADFGTALGDKLALAIVDGPGKDSFPIAGYTYLLLYMDQQDCT
                     KAQKIIGFLKYAFGSDGDKSATDLSYVALPQAVKDQVNAKLALITCQGKALPK"
     gene            2705..>3367
                     /gene="pstC"
                     /locus_tag="VFF70_04930"
     CDS             2705..>3367
                     /gene="pstC"
                     /locus_tag="VFF70_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZY44077.1"
                     /translation="MATDSRLNAPARLSASRLSRAWQHGDRFYKVLITLAALSVLLLV
                     VAIGYELWHSSALSREKFGLNFITTNDWDPVAESFGALPFILGTLVTSIIALIIAIPI
                     GLGVAIFLAELAPNWLRQPIGFVVELLAAVPSVIYGLWGLFVFIPIFVKPLAKDLNSA
                     LGFLPLFQGPVFGPSRLAAGLLLAIMILPTIASISRDVFRAIPNTQREASLALGATRW
                     EMI"
BASE COUNT          762 a          910 c          888 g          807 t
ORIGIN      
        1 cgtcggcgta gcgcacggct tgagtgcagg caaggctagt cttgcctacg ccgcccttgc
       61 cggagaagaa aataaactgc gtcatgcggt tgctccgtct aaataggatt tgatttcggt
      121 cagcgtggga taggctccta ccttaacgac tagcccgcgc acaaccgtga tgggcagtgc
      181 ggccatttgc ttttcgcgca ccagccgcat cacatcgggg ttgctcagga aagcctgcgg
      241 atggctggtc atctgatagc gctcgacgtt caaaccatct gtctgcagtg cgacgatcat
      301 ctcactgaca tccagcaaag tttgatcaag cgttgggcca cacaggccgg tcggacagca
      361 catcggtgga tcaaacaatt ccacaacagc agcttgagga tggatgggcg cggcatcgaa
      421 caggctaatt gtatctgaca tagtcattca atcttccttg atgagttaat aatattcatt
      481 atgcgccttg aggttgatgc tccagcgcgg tcgtgatcca cgtcgtcact tcggcccgat
      541 tcggaatccg tcctgaacaa accaactgct cattgattac cagaccgggc gtcgataaaa
      601 tgggataacg catgatctcc gcgtagtcgg tgatcttttc gatgtacgca tcgacatcaa
      661 gttccttcac gaccaactgc gctaactgct ccatacgttt gcagttggaa cagccggggc
      721 ctaatacttt gatcgtcaac atgcttgttc tcctaataaa tcaaatttga atcttcgcaa
      781 ctttcaactc aaccgttgga ttgcacttcg ggcacgggca attgtccagc gaggcagact
      841 tcatcgactt cacgcggctg aggcggtgag tgccattcaa gcgcagggct gcatcgatga
      901 tctcaaacac ctgcggtcgt gtcacacggt agtaaatatt ccaaccctcg cgtcgatctt
      961 ggatcaggcc agcttcacgc agtgcggcta attgctgtga gatgtacgcc tgccgataac
     1021 tcaacgccgc ctcgatgtgg cacacgcacg cctcaccgtt tcgcaagacg tcaagaatct
     1081 ccaggcgagc cgggtgcatt aagactttga aaagttgggc cgatcgatcg aattcgttca
     1141 tggccaaaac cattgcaaca tattcaatat tccgaatatg attatgccgc tgaatcgatc
     1201 gttgcgacac gctagaacgt cacgacttcg atgtgacaat cggcttgatc gcagacgttt
     1261 tgagaatata ccttatctct atcttcaata cgggcgaaat gattcatctt atacaacact
     1321 gaatttttct tttggaaaag ttaaataacc ttaacactca cttaaccgcg gcttgatttg
     1381 cgtctgttac agtagcaacg tgaataaatt cgctgaaaat caaatcatta aggagcagaa
     1441 gaatatgcaa cgcaatactc gtttcgtagc gatcacgctg ttgatcgcag tcttagttag
     1501 tgcctgtgcg cccgccgcaa cgccagccgc tccggcagcc acgcaaccgc cggttgttca
     1561 gacggttgtg gtgcaaccaa ccgcggggcc agcgcagccc acggccgtgc ccgctggatc
     1621 gatcacgatc aatggcgcgg gtgccacctt ccccttcccg ctgtacagca agtggttcta
     1681 cgattacgct ttcgtggata cctcggtgaa gttcaactat caatcgattg ggtcaggcgg
     1741 cggcattcaa cagatcaccg ccaagacggt tgacttcggc gcgtccgatt cgatcctcaa
     1801 cgatgcgcaa tacactgcgg cgccgggcat tcaaatgttt ccgaccgtag ccggtgcgga
     1861 agcgatcgtt gttaacctca agggtgatga cggcaatccg atcactacga cgctgaactt
     1921 cccgtacacc tcggtggccg acatttttgc gggcaagatc accaagtgga atgatccggt
     1981 gttgactgcg gcgaatccca atgtgactct gcccaacaaa gatatcatcg tcgtgcaccg
     2041 atcggatggt tccggcacga ccttcatctt caccagctac ttgtcgagcg tgagcgcgga
     2101 ctggaagacg aacgtcggcg cggcctcatc ggtgaagtgg ccggttggac tgggcggcaa
     2161 aggcaatgaa ggtgtgtttg gtacggtctc acaaaatgac ggcgcgatag gctacgtgga
     2221 gcttgcgtat gcgcttcaga acaaggccac catcaacgcc ctgcagaaca aagccggtga
     2281 atttgtgaag ccgtcgatcg cgagtacgca agcggccatg gccgacttcg gcaccgcgct
     2341 gggtgacaaa ctggcgctgg cgatcgtcga tgggccgggc aaggacagct tcccgatcgc
     2401 gggttatacc tacctgctgc tgtacatgga tcagcaagat tgcaccaaag cgcagaagat
     2461 catcggcttc ttgaaatatg ctttcggatc agatggcgac aaatcggcga cggacttaag
     2521 ctatgtggct ctgcctcaag ccgtcaaaga tcaagtcaat gcgaaactgg cgctgattac
     2581 ttgccagggc aaagcattac cgaagtaagt attattggaa ctagatgtag tagtacaccc
     2641 tccctcggct gtgaatgacc gagggagggt cttgtgtcaa tatgtgagtc ttgaggagaa
     2701 gtctgtggca actgattcac gcctaaacgc gcctgctcgt ttgtccgcct cgcgtctcag
     2761 tcgcgcctgg cagcatggcg atcgcttcta caaggtattg atcacgctag cagccttgag
     2821 tgtgctgctg ctggtcgtag ccatcggcta cgaattgtgg cacagctcgg cgctatcacg
     2881 cgaaaagttc ggcttgaact ttatcacgac caacgattgg gatccggtcg ccgaatcatt
     2941 cggcgcactg ccctttattc tcggcacgct ggtcacttcg atcattgcgt tgatcatcgc
     3001 catcccgatc ggcttgggcg tggcgatctt tctggccgaa ctcgcgccga attggctgcg
     3061 tcaaccgatc ggttttgtgg tggaattgtt agctgctgta ccaagcgtta tctacggcct
     3121 gtggggactg ttcgtcttca ttcccatttt tgtcaaaccc ttagccaaag acctgaatag
     3181 cgcgcttggt ttcttgccgt tgtttcaagg tcccgtcttc ggtcccagcc gcctggccgc
     3241 cgggttactg ttagcgatca tgatcttgcc gacgattgcc tcgatcagcc gcgatgtgtt
     3301 tcgcgcgatt ccaaatactc agcgtgaagc ttcgctggcg ttaggcgcaa cacgttggga
     3361 aatgatc
//
LOCUS       DASRTJ010000235         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_119337,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000235 DASRTJ010000000
VERSION     DASRTJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119337"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFF70_04935"
     CDS             complement(<1..556)
                     /locus_tag="VFF70_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZY44078.1"
                     /translation="MHAEILATGSELLLGEIVDTNSAFIARKLREIGLNLFYKTIVGD
                     NAERMIEALQIALNRSDVIITTGGLGPTVDDVTRSAVARAIKAELEFRPDLLEQIEAR
                     FRAYNVPMSVNNKQQAYIPAGATPIHNPVGTAPCFSVEYQGKLIISLPGVPREMEYLI
                     EYAVLPFLKEKLDLHAVIVVRTLHV"
     gene            complement(691..1194)
                     /locus_tag="VFF70_04940"
     CDS             complement(691..1194)
                     /locus_tag="VFF70_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY44079.1"
                     /translation="MEKVDILKRTDIFYDLTGPQLEMMASLCEDRSVKLGEIVFEENS
                     NGDEMFVIARGSVEVLVDPTIVGGPSAKKRGSGPVTIATLRSGQSFGEVALVDQGLRT
                     ASARCAETETHLLLIPRAKFNKLCDTYPELGYRVMRNIAADLAFKIRGSDLAIREQLL
                     WRPRPND"
     gene            1313..>1552
                     /locus_tag="VFF70_04945"
     CDS             1313..>1552
                     /locus_tag="VFF70_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44080.1"
                     /translation="MNRILVLYLSIGSGHQVGAEAIATALRSHSTAEVICRDPIAEHS
                     TLLSSIMNTSNSLSAVIFPRLYTKIWSGGQVRSITN"
BASE COUNT          349 a          461 c          378 g          364 t
ORIGIN      
        1 ccacatgcag cgtgcgcacg acgatcaccg catgcagatc gagtttctct ttcaagaacg
       61 gtaggaccgc atactcgatc aagtattcca tttcgcgcgg cacgcccggc aggctgatga
      121 tcaacttgcc ttgatattcg acgctgaagc agggcgccgt cccgaccgga ttatgaatcg
      181 gtgttgcgcc cgcggggata tacgcttgct gtttgttatt cacgctcatc ggcacgttgt
      241 aggcgcgaaa gcgcgcctcg atctgctcca ataaatccgg ccggaactcc aattcggcct
      301 tgatcgcccg tgccaccgcc gatcgagtca cgtcatctac ggtcgggccg agtccgcccg
      361 tcgtgatgat cacatccgat cggtttaacg cgatctggag cgcttcgatc atccgctctg
      421 cgttgtcgcc gacgatcgtt ttataaaata gattcaaccc gatctcgcgc agcttgcggg
      481 caatgaacgc cgagttcgta tcgacgatct cgcccaataa caattcgcta ccggtggcaa
      541 gtatctcagc gtgcataatc caatcctccg acagttttta gccgattacc cgatcagttc
      601 gtttgaacct gctggaggat aatcgatcat gatctcagcc tgcatcagct ataaaaaaac
      661 tgcgaggcat atcttgcctc gcaggtctgt tcagtcgttt gggcgtggcc gccacaacaa
      721 ctgttcgcgg attgccagat cgctgccgcg aatcttaaag gctagatcgg ccgcaatgtt
      781 gcgcatcaca cgatagccca attcaggata agtgtcacac aacttattga acttggcgcg
      841 cgggatcagc agcaaatgcg tctcagtttc cgcgcagcgc gccgaggccg tgcgcagacc
      901 ttgatcgact agagccactt caccaaacga ttgtccagag cgtaaagtcg caatcgtcac
      961 cggtccgctg ccgcgtttct tcgccgaggg tccgccgacg atcgtcggat cgaccagcac
     1021 ttccaccgag ccgcgcgcga tcacaaacat ttcatcaccg tttgaattct cttcaaagac
     1081 gatctcgccc agcttcaccg atcggtcctc acacaacgaa gccatcatct ccaactgtgg
     1141 gccggtcaaa tcatagaaga tatccgtgcg tttaagaata tcgaccttct ccatgctgtg
     1201 ctcctgggct gaaattgctt gctcgtgtgt gctcgtattc tagcataaag tagattgaat
     1261 aggcaaccgc acacgcttga ctctgcctga cacctcggtt accatcaagc tgatgaatag
     1321 aattctcgtc ctgtatttat cgatcggttc cggtcatcaa gttggcgcag aagcgatcgc
     1381 caccgcgtta cgatcgcact ccaccgccga agtgatctgc cgcgatccga ttgcggaaca
     1441 cagcaccttg ctgagttcga tcatgaatac atcgaactca ctcagcgccg tgatctttcc
     1501 gcgactgtat accaaaattt ggtcgggcgg tcaggtccga tcaatcacga at
//
LOCUS       DASRTJ010000236         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_51748,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000236 DASRTJ010000000
VERSION     DASRTJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51748"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1120
                     /gene="meaB"
                     /locus_tag="VFF70_04950"
     CDS             77..1120
                     /gene="meaB"
                     /locus_tag="VFF70_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZY44081.1"
                     /translation="MPSDIAALIDRALAGDRRSLARLISRVENGGPDSWSALSIVYPR
                     TGRAQIIGITGSPGTGKSTLVGEIAKLYRAEKLTVGIVAVDPTSPFSGGALLGDRIRM
                     RDLAGDAGVFIRSMATRGSLGGLARATTDVVKVLDAAGYNVILIETVGAGQSEIDIAR
                     TADTVVVVEAPGLGDEVQAIKAGILEIADILVVNKADRDGAQSTVNALHAMIDLGTPH
                     NRTLHHGALMELNSASGFHTGIAGRSQNSDRWYPPILMTIAAEGKGVDKVVEAIGQHH
                     QYLIDQPEGQQRVRARVSIELETILRESLLNRLLAGLGSQAIDRAIDQVVKREIDPYT
                     AVEQLQAAAQVRA"
     gene            1117..1752
                     /locus_tag="VFF70_04955"
     CDS             1117..1752
                     /locus_tag="VFF70_04955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZY44082.1"
                     /translation="MSVRLLLIRHARSTWNAEGRLQGIADPPLDAVGLEQANRLADRL
                     KEQPIAAIYSSPLQRARSTAEIIGQKFPVAPLTFDDRLMEYNIGVLTGLTWADIAIKH
                     PDFAARWSEDAWGAPIPESEGQVLFRARVMAVMHEISLQHVDQVVAVVSHGGTFGAYL
                     AASLGLDANRRDPFHFGNTSLSIVDLDGELIKIVALNDAHHLRGLPQISLI"
     gene            1791..2765
                     /locus_tag="VFF70_04960"
     CDS             1791..2765
                     /locus_tag="VFF70_04960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZY44083.1"
                     /translation="MAGSLHAFGEIKIFCGSSCPALADEIGQYLKVPISPREIIQFPN
                     DNIFVRLLTSVRGQDVFIIQTLSSPVNENIMELLIMLDTVKRANAGRITAVMPYYAYG
                     RSDKKDQPRVPITARLLADLVITAGADRYLTLDLHAGQIQGFFSIPGDEVSTFHLLSE
                     YFISKKLNRVPNGAVIVTADLGYAKKGRNFAQVLSIPMAFVEKRRTGNDANAEALTVI
                     GDVVGKTVIVVDDEVDTGGSVSGAINILKESGAAEIYLCFTHAVLSPPAIDRLRGLPI
                     KEIVTTNSVPIPKEKTLPNMTVLSVAPLLGEVMRRIHEGSSVGQMFNE"
     gene            2868..>3095
                     /locus_tag="VFF70_04965"
     CDS             2868..>3095
                     /locus_tag="VFF70_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44084.1"
                     /translation="MLKNLFGRSSEDREVAKLRPVVAQINELEPEFEKMSADELRAVT
                     VEFRSELNDSLHDRRQELAEARAEAQAEDDTD"
BASE COUNT          684 a          758 c          949 g          704 t
ORIGIN      
        1 gcgggcgtga ccggcgtgtt cggccccggc accaacactg accacgtcgt cgagttcatt
       61 cgtgatcaat tcaaagatgc ccagtgacat cgccgcgttg atcgatcggg cgttagcggg
      121 cgatcgacga tcgttggcgc gcttgatcag ccgtgtggaa aacggcggtc ccgatagttg
      181 gtcggcgctc tcaattgttt acccgcgtac cggccgggcg cagatcatcg ggatcaccgg
      241 atcgcccggc accggtaaaa gcacactggt aggggagatt gcaaaactgt atcgcgctga
      301 gaagttgaca gtgggtatcg ttgcggtcga tcccacttcg ccgttttccg gcggggcttt
      361 attgggcgat cgaatccgca tgcgcgacct cgctggtgat gcgggggtct tcatacgatc
      421 gatggcgacg cgcggcagtt tgggcggctt ggcgcgcgcc acgacggatg tggtgaaagt
      481 tctggatgcg gcgggttaca atgtgatctt gatcgagacg gtgggcgccg gacaatcgga
      541 gatcgacatt gcgcgcacgg cggatacggt ggttgttgtt gaagccccgg gtctgggcga
      601 tgaagtgcaa gcgatcaaag ccggtatatt ggaaattgcg gatatcttgg tcgtgaacaa
      661 agccgatcga gatggcgcgc agagtacggt aaatgcgctg catgcgatga tcgatctggg
      721 cacgccgcac aatcgaacgc tgcatcatgg cgcattgatg gaacttaaca gcgcgtcagg
      781 cttccatacc ggcatcgcag ggcgatcgca aaattccgat cgttggtatc cgccgatctt
      841 gatgacgatt gcggccgaag gcaaaggcgt cgataaggtt gtcgaagcga tcgggcagca
      901 ccaccaatat ctgatcgatc aaccggaagg ccaacagcgc gttcgggcca gagtatccat
      961 cgagttggag acgatcttgc gcgagagttt actcaaccgc ttattggccg ggcttggatc
     1021 acaggcgatc gatcgtgcga tcgatcaagt cgtgaagcgt gagatcgatc cctatacggc
     1081 ggtggagcaa ctccaagcgg cggcgcaggt tcgcgcatga gtgtgcggtt attgctgatc
     1141 cgacatgccc gatcaacttg gaatgccgag ggtcgcctgc aaggaatcgc cgatccgccg
     1201 ctagatgccg ttgggctaga gcaagccaat cggttggcag atagattgaa agagcagccc
     1261 attgccgcga tttattccag cccgctgcag cgcgcacgat cgaccgcgga gatcatcggc
     1321 cagaaatttc cagtggcgcc gttgaccttc gacgatcgtt taatggaata taatatcggt
     1381 gtgttgaccg gtttgacctg ggccgatatt gcgatcaagc atcctgattt tgcggcgcgt
     1441 tggtcggagg atgcttgggg cgcgccaata ccggagagtg aaggtcaggt gctttttcgc
     1501 gcgagggtga tggcggtgat gcacgaaatt tcgttacagc acgttgatca agtggtggcg
     1561 gtcgtgtcgc acggtggaac cttcggcgct tatttagcgg caagcttggg tttggatgcc
     1621 aatcgtcgtg atccatttca ttttggtaac acctctttga gcattgtcga ccttgatggc
     1681 gaattgatca aaattgtggc gctaaacgat gcgcatcatc tgcgcggtct gccgcagatc
     1741 tctttgattt agtgcagacc tatccctttg aacggacgga cttaatcact atggctggat
     1801 cgctgcatgc gtttggtgaa atcaaaatct tctgtggctc ctcttgccca gcgttagcgg
     1861 acgagatcgg tcagtatttg aaagtgccga tcagtccacg tgagatcatc cagttcccga
     1921 atgacaacat tttcgttcgc ttgttgacga gtgtgcgcgg ccaggatgtg ttcattattc
     1981 agacgctgag ctcgccggtg aacgagaata tcatggagct gctgatcatg ttggatacgg
     2041 tgaaacgggc gaatgccggc cgcatcacag cagtgatgcc gtattacgcg tatggtcgca
     2101 gcgataagaa ggatcagccg cgtgtgccaa tcacagcgcg cctgttggcg gatttggtaa
     2161 tcaccgccgg agctgatcga tatctgaccc tcgatctgca tgccgggcag attcaaggtt
     2221 tcttcagtat tccgggcgat gaagtcagca cctttcattt gttaagcgaa tacttcatct
     2281 ccaagaaact gaatcgagta ccgaacggcg ccgtcatcgt caccgcggac ttgggctatg
     2341 ccaagaaggg ccgcaacttc gcgcaggtgt tgagtattcc tatggccttc gtcgagaagc
     2401 ggcggacagg aaacgacgca aacgccgagg cgttgaccgt aatcggcgat gtagtaggca
     2461 aaacggtgat cgtcgtcgat gacgaggtcg atacgggcgg atcggtttcg ggcgcgatca
     2521 acattttgaa ggaatctggc gcggcggaga tttacttgtg tttcactcac gcggtattat
     2581 cgccgccggc catcgatcga ttgcgtggtc tgccgatcaa ggaaattgtg acgacgaaca
     2641 gcgtgccgat cccgaaggag aaaaccctgc caaatatgac ggtgctctct gttgcgccgc
     2701 tgctgggtga agtcatgcgg cggattcacg aaggctcgtc tgtggggcag atgtttaatg
     2761 aatagccaaa attggctatc caaggtttct ccgagatcag gtaagattga cagtaatcgg
     2821 ttcatattta tccagtatca gtagcgatgg gaaattaact gtaaattatg ttgaaaaatt
     2881 tatttggaag atctagcgaa gaccgtgaag tcgccaagct gcgaccggtc gtcgctcaaa
     2941 tcaacgaact cgaacccgaa tttgagaaga tgtcggccga tgagctgcgc gccgtcacgg
     3001 tcgagtttcg atccgagtta aatgactcgt tgcacgaccg gcggcaggaa ttagccgagg
     3061 cgcgcgccga agcgcaggcc gaagacgata ccgat
//
LOCUS       DASRTJ010000237         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_13655,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000237 DASRTJ010000000
VERSION     DASRTJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13655"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2491)
                     /gene="ptsP"
                     /locus_tag="VFF70_04970"
     CDS             complement(8..2491)
                     /gene="ptsP"
                     /locus_tag="VFF70_04970"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZY44085.1"
                     /translation="MVNLVLVSHSPSLAAGVAELARAMTQQSSIVIATSAGTGDPANP
                     LGTNPEDIQRAIEAAYSDDGVLVLMDLGSAVIGAEMALEFLPDDQRTKVRLCEAPMIE
                     GAVAAAVQASLGGTLDQVASEALGALASKAIHLAHEVSAPTSISILAPTIAGQSLEIK
                     IQNRLGLHARPAAQFVQMAARFKSDIAIARVSNAAKSVNAKSINAVASFGVRQNEMIQ
                     ISADGSDAAEALSALKSLIEAKFGEDREPIEPARPIELKPIKPAITGELIGIAASPGI
                     AIGPAALIQFIEPKIDRRFIDNPSAEWSRLEAALFAVRVSTRQLREDVARAANDYEAA
                     IFDAHLMFLDDPELIAAARKAIYTEHINAEAAWHTVIDTSVAAFEAIDDPYLRARAAD
                     VADIGRQVLSQLTGESTQATLSHAGILIATDLAPSITARLDRSKVLGICTERGGPTSH
                     SAILARTLGIPAVVGLGASIMNVVDDVSLIIDGQSGQVWIDPAADLITQYTTRMKDWQ
                     SAREVTRQSSTSPAITRDGRSIEVVANIGSVADARTAIEHGAEGVGLLRTEFLFLDRS
                     TAPTEDEQFATYRSIAEVMGQRPVIVRTLDVGGDKPLPYLDLGREENPFLGQRAIRLC
                     LNRPELFKTQLRAILRASVGHNLKIMFPMIAEVSEVRRARELLNDSRAELKAKNMPVA
                     DSIEIGIMVEIPAAALLAEAIASEVDFFSIGTNDLTQYTLAAERGNRSVAHLQDALHP
                     AVLRQIQHTVQSATAVGKWVGVCGELAGDPLAVPILIGLGVRELSMASASIPQAKQII
                     REWSQSEAAALAAKAVNLASAEAVRRIIS"
     gene            complement(2518..3102)
                     /locus_tag="VFF70_04975"
     CDS             complement(2518..3102)
                     /locus_tag="VFF70_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HZY44086.1"
                     /translation="MANLHHHFTRRLTMIDWESRWAVYAEEDYQFVIDRIGSQDVVLD
                     IGAGDLQLARRLASIVKKVYAIERNPQILAQSDRSRGFSNLKVICADALTYPFPNDVT
                     VGVLLMRHCAHFSEYATRLIEAGCPRLITNARWGMGVEAIDLRSGVSYDPDRMGWYAC
                     RCGSIGFTPGDPQLIDQQILESGLEVIDCPKCRT"
     gene            complement(3066..3434)
                     /locus_tag="VFF70_04980"
     CDS             complement(3066..3434)
                     /locus_tag="VFF70_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44087.1"
                     /translation="MQRTLTRERASSWSWILQAITGVLVIVLVGVHMIFQHFVAAGGL
                     RTYTDVVAYIRTPFFLGWETIFLIVVTWHALLGVRAIVLDFGLKRSIERTFNWILLII
                     GVGTIVYGLWLTFTIISPAG"
     gene            complement(3469..3864)
                     /locus_tag="VFF70_04985"
     CDS             complement(3469..3864)
                     /locus_tag="VFF70_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44088.1"
                     /translation="MSVEVQPKPVTRKPAQWIDLRGRQVGFWAYLAMRLSGIGLVAYL
                     YLHLVILNQLTAGPGAWDGFVALARSPIFLALDVVLIIGLLVHGLNGLRLVLVGFGIG
                     VPRHKTMLWIGVALAVLLTVLSALAIFSK"
     gene            complement(3929..>4374)
                     /locus_tag="VFF70_04990"
     CDS             complement(3929..>4374)
                     /locus_tag="VFF70_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY44089.1"
                     /translation="FPLVTDLVVDAAPLYERTREVGTPIMRKSEPLRENGLRVLPEGI
                     ADFNRFENCIECGLCLSACPSMAADPYYLGPVALAMAERALAEPRGIDRQSIIDLIDD
                     EHGLWRCHSAFECTEVCPSNVDPAGAIMRLKRIVAAEKFKKLFGR"
BASE COUNT         1022 a         1291 c         1139 g          922 t
ORIGIN      
        1 agtgtcttta agaaattatt cgacggacgg cttcggcgct agctaagttt acggctttgg
       61 cggcgagagc cgccgcttcc gattggctcc actcgcggat gatctgcttg gcctgcggga
      121 tcgaagcgga agccatgctg agttcgcgca ctcctaaccc gatcaagatc ggcacggcca
      181 gcggatcgcc ggccagttcg ccgcacacgc ctacccactt gcctactgct gttgccgatt
      241 gaacggtgtg ttgaatttgt cgcaacacgg caggatgcaa ggcatcctgc aagtgtgcca
      301 cacttcgatt gccgcgttcg gcggcgagcg tgtattgcgt gagatcgttt gtgccgatgc
      361 tgaaaaaatc gacctctgat gcaatcgctt cagccagcag tgccgccgcc ggaatctcga
      421 ccatgatccc aatctcgatc gagtcggcca cgggcatgtt cttcgccttc aattcggctc
      481 ggctgtcatt taataactcg cgcgcgcggc gcacttcaga aacttcggcg atcatcggga
      541 acatgatctt gaggttgtga cccacggacg cgcgcaagat agcccgtaac tgcgttttga
      601 aaagttccgg tcgattcaaa cacaaccgaa tagctcgctg ccccaaaaac ggattttctt
      661 cgcggcccag atcgagatac ggtagcggct tgtcgccacc gacatcgagc gtacgcacga
      721 taaccggacg ctgacccatc acctcggcaa tggatcgata ggttgcaaac tgctcgtctt
      781 cagtcggcgc agtgcttcga tcgagaaaca gaaattcagt ccgcagcaag ccaacgcctt
      841 ccgcgccatg ctcgatcgcc gtgcgcgcat cggccacact gccaatgttg gcgacaactt
      901 cgattgatcg tccgtcacgc gtgatcgctg gcgatgtgct tgattggcgt gttacctctc
      961 gcgccgattg ccagtctttc atgcgggtcg tgtattgcgt gatcaagtct gccgctggat
     1021 cgatccacac ttgtccactc tgtccatcga tgatgagcga tacgtcatcg acaacgttca
     1081 taatgctcgc gcccaaccca acgacggccg gaatgcctaa cgtccgcgcc aggatggcgc
     1141 tgtgcgaagt aggtccaccg cgctcggtgc aaattcccaa caccttcgat cgatcgagac
     1201 gtgccgtaat cgatggcgcg agatcagtcg cgatcaaaat accggcgtgt gataatgtcg
     1261 cctgcgtcga ttctcccgtg agctgcgaca aaacctgccg accgatgtcg gccacatcgg
     1321 ccgcacgcgc acgcaggtac ggatcatcga tcgcttcaaa ggcggcgacc gacgtatcaa
     1381 tcaccgtgtg ccacgcggcc tcggcattga tatgctcggt gtagattgct ttccgcgcag
     1441 ccgcgatcaa ctcaggatcg tccagaaaca tcaaatgggc atcgaagatg gcggcttcat
     1501 aatcgttggc ggcgcgcgcg acatcttcgc gcagctggcg cgtcgacacc cgcaccgcga
     1561 atagcgccgc ttccaaacgc gaccattcag cggaaggatt atcgatgaaa cgccgatcaa
     1621 tcttgggttc gatgaattga atcagtgccg caggtccaat cgcaattccg ggcgaagctg
     1681 caataccgat caattctccg gtgattgcag gtttgatcgg cttcagctcg atcgggcgtg
     1741 ctggctcgat cggttcacgg tcttcgccga acttcgcttc gatcaatgac ttcaacgccg
     1801 acaaggcttc agccgcatcc gaaccatcgg ccgagatttg aatcatttca ttctgccgca
     1861 caccgaacga ggccaccgca ttgatgctct tggcattcac ggacttcgct gcgttggaca
     1921 cgcgcgcgat cgcgatgtcg gatttgaacc gagcggccat ctgcacgaat tgcgctgccg
     1981 gtcgggcgtg cagacccaat cgattttgaa ttttgatctc aagtgattgc ccggcgatcg
     2041 tcggggcgag gatcgatatc gatgtaggag cagaaacttc gtgagccaag tggatcgctt
     2101 tagaggccag cgcgccgagt gcttcgctgg ctacttgatc gagtgtgccg cccaggctgg
     2161 cctgcacagc ggcggcaacc gcgccttcga tcatcggcgc ttcgcacaaa cgaactttag
     2221 tgcgttgatc gtcaggcaaa aattccaatg ccatctctgc gccgatcacg gcgctgccaa
     2281 gatccatcaa cactaaaact ccatcgtcgc tgtaggccgc ttcgatggcg cgttgaatat
     2341 cttcaggatt tgtgccaagt ggattagccg gatcgcctgt gccggcggaa gtggctatga
     2401 caatcgaaga ttgctgcgtc atggcgcgcg ccaactcggc cacacccgca gctaacgatg
     2461 gactgtgaga cactaagacc agattaacca tatagatgct gaagcgcccc gcccgcttta
     2521 cgtcctacac ttcggacaat cgatcacttc caacccagat tccaaaatct gttgatcgat
     2581 caactgcgga tcgcctggtg tgaaaccgat cgatccacag cggcacgcgt accaacccat
     2641 gcgatcagga tcatagctta cgcccgatcg gagatcgatc gcttcgacgc ccatgcccca
     2701 acgcgcattg gtgatcaatc gcggacagcc cgcttctatc aggcgcgtgg catattcact
     2761 gaagtgagcg cagtgccgca tcaacaatac gccgacggtg acatcattgg gaaagggata
     2821 cgtcaacgca tcggcgcaaa tcactttcaa gttgctaaac ccgcgtgatc gatcggattg
     2881 agcaagaatc tgcggattgc gttcaattgc atatactttc ttgacaattg atgcgagacg
     2941 gcgagccagc tgcagatcgc ctgcgccaat gtcgagcacg acatcctgcg acccgatgcg
     3001 atcgattacg aactgataat cttcctccgc atacactgcc catctcgact cccaatcgat
     3061 cattgtcagc cggcgggtga aatgatggtg aaggttagcc acaagccgta aacgattgtg
     3121 cccacgccga tgatcaataa tatccaattg aaagtgcgtt cgatcgaacg cttcagtcca
     3181 aagtccaaaa cgatcgcgcg cacgcccaac agcgcatgcc acgtcaccac gatcaagaaa
     3241 atcgtttccc agcccaagaa aaacggcgtg cgaatgtaag cgacaacatc agtgtaagtg
     3301 cgcaggccgc cagccgcaac gaagtgttga aagatcatgt gcacaccgac gaggacgatc
     3361 actaatacgc cagtgatagc ctgcaagatc cacgaccaac tgctggctcg ctcgcgagtt
     3421 aatgttcttt gcatgttgtt cctcttgagg gcgagcagcc gctcgcccct actttgagaa
     3481 tattgccagc gcgctcaata cagttaggag caccgctaac gccacaccaa tccacaacat
     3541 tgttttgtga cgcggcacgc cgatgccaaa gccaaccaat acaagccgca aaccgttcaa
     3601 gccatgtact aacaaaccta tgattaaaac tacatctaat gccagaaaga tcggcgatcg
     3661 agccagagcg acaaagccat cccacgcgcc cggcccggcg gtcagttgat ttaagatgac
     3721 taaatgcaaa tagagatacg cgaccaaacc gatgccgctt aagcgcatcg ccagatatgc
     3781 ccagaagcct acttgcctgc cacgcagatc gatccattgt gcgggcttgc gagtgacggg
     3841 tttcggttga acttcaacgg acatctatgc ctcctgaatg cttatcgcat attgcttatc
     3901 gcaatgacat gcagcgatat gctatcggct atcggccaaa taatttcttg aacttctctg
     3961 ccgccacaat ccgcttcaag cgcatgatcg cgcccgccgg atcgacattc gacgggcaga
     4021 cttcagtaca ttcgaaggcc gaatgacagc gccacagtcc gtgctcgtca tcgatcagat
     4081 caatgatcga ttggcgatca atgccgcgtg gttcggctaa tgcgcgttcc gccatcgcca
     4141 atgcgaccgg tcctaaataa tatggatcgg ccgccattga tggacaagcc gacaggcata
     4201 agccacactc gatgcagttt tcaaaacgat tgaagtcggc aatcccttcg ggcagaacgc
     4261 gcagaccgtt ttcacgcagc ggttcagact tgcgcatgat cggcgtgccg acctcgcgcg
     4321 tgcgctcata taatggcgca gcatcgacaa ccagatcggt gacgagcgga aagt
//
LOCUS       DASRTJ010000238         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_195155,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000238 DASRTJ010000000
VERSION     DASRTJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195155"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VFF70_04995"
     CDS             complement(<1..1101)
                     /locus_tag="VFF70_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY44090.1"
                     /translation="MRILINGWFIDQPHTGSGQYTLQLLKHLPRVAPEHKYSIVVPKG
                     NSFEIIDLHPDSNLQSLTSASQRPKSNIRKMIFEQSIVPRAASAYQADLIHVPYWAPP
                     LRSSVPIVVTIHDLIPLLLKEYRGGPLVRMYTGLVSAAARGAALILTDSNASRLDIIQ
                     HLKIDAARIRTIYLAADEKFSTHADPIDHAALHQNYDLPDEYVLYLGGFDARKNIETL
                     LQIYTWAQDTIEIDYPLVIAGQLPDRHDAFFHDPRIIAKQLEVEDVVRCIGPVAEEDK
                     AALYQAARAFLFPSLYEGFGLPPLEALACGLPVIGSNASALSEIVGAAGVLVDPKDAR
                     AMAGALIAVVTENKLHDDLADRAVQQAAKFSWE"
     gene            complement(1156..1554)
                     /locus_tag="VFF70_05000"
     CDS             complement(1156..1554)
                     /locus_tag="VFF70_05000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44091.1"
                     /translation="MITYSDSHDIDLQQLSVLFNSVGWERRTADRDRLAQLVRGSMWV
                     VSAQHESKLVGFARAVSDGATNAYVSTVAVLPEYQGRGIGREIMRRLIDGRDGIQFVL
                     HANDNATAFYLHLDLGFEPINNMLRRPRKF"
     gene            complement(1601..2599)
                     /gene="galE"
                     /locus_tag="VFF70_05005"
     CDS             complement(1601..2599)
                     /gene="galE"
                     /locus_tag="VFF70_05005"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZY44092.1"
                     /translation="MNILVTGGAGYIGSHVVEELCESGESVVVIDNLYTGHRAAVHPK
                     ATFIEGDLKDTALLQRLFDDHSFDGVMHFAAYTLVGESMAQPFLYVGDNVTNALNLLR
                     AMIDHNVKRFILSSTANLFDDPARMPIDEAERIVPGSPYGESKYIVERILHWLDRTSQ
                     LRYAALRYFNAAGASPRGNRGEHHAHETHLIPLILQVALGQREKISVFGNDYSTPDGT
                     CIRDYVHVTDLAQAHILALRALDQGSRTYNLGNGQGHSVKEVIDAARSITGREIKMEF
                     GPRRSGDPAILIASSDKIRRELGWQPRYEALHDIIATAWDWHRSHPNGYSDLTAKQ"
BASE COUNT          648 a          734 c          828 g          653 t
ORIGIN      
        1 ttcccacgag aatttggccg cttgttgaac agcgcgatcg gccagatcgt cgtgcagttt
       61 gttttcggtg acgaccgcga tcaacgcgcc ggccatcgcc cgtgcatctt tcggatcgac
      121 caagacgccc gcagcgccga cgatctctga cagcgctgac gcgttcgagc cgatgacggg
      181 caggccgcag gccagggcct cgagcggcgg caggccgaag ccttcatata atgaagggaa
      241 tagaaacgcg cgcgcggctt gatacaaagc ggctttatct tcctcggcca ccgggccgat
      301 acagcgcacg acatcttcca cttcaagttg tttggcgatg atgcgcggat cgtgaaagaa
      361 ggcgtcgtgt cgatccggga gttgtccggc aatcacgagc ggataatcaa tctcgatcgt
      421 atcctgtgcc catgtataaa tttgcaagag cgtttcaatg ttcttgcgcg catcgaagcc
      481 gccgaggtac aacacgtact cgtcgggcag atcgtagttc tggtggagcg cggcatgatc
      541 gatcggatcc gcgtgcgtcg agaatttttc gtcggcggcg agataaatcg tccgtatgcg
      601 ggctgcatcg atcttaagat gctgaatgat gtccagtcgg ctggcattcg aatcggtgag
      661 gatcaacgcc gcgccgcgag ccgccgcact gacgaggccg gtgtacattc gaaccagcgg
      721 cccgcctctg tattctttca acaacaacgg aatgagatcg tgaatggtga caacgatcgg
      781 cacgctcgat cgcagcggcg gcgcccaata cggcacgtgg atcagatcgg cttgataggc
      841 tgaagccgcg cgcggcacga tcgattgttc gaagatcatt ttgcgaatat tcgattttgg
      901 acgctggctg gcggatgtta gagattgaag gttagagtct ggatgaagat cgatgatctc
      961 aaacgagtta cctttaggga caacgattga gtatttatgc tcaggcgcga cgcgcggcag
     1021 atgtttcaac agctgcaagg tgtattgtcc gctgccagtg tggggttgat cgatgaacca
     1081 gccgttgata agaatacgca tattggaacg ccgatttcct ccggcatttg acagccgact
     1141 aaattcggca ctcccctaaa attttctggg ccggcgcagc atgttattga tcggctcgaa
     1201 gccgagatcg agatgcagat aaaacgcagt ggcgttgtcg ttggcgtgca ggacgaattg
     1261 aatgccatcg cgtccatcga tcaagcgtcg catgatttcg cggccaatgc cgcggccttg
     1321 atattccggc aacacggcga cggtgctgac gtaggcgttg gttgcgccat ccgatacggc
     1381 gcgcgcgaag ccgacgagtt tgctttcatg ctgcgcggac acgacccaca tcgatccgcg
     1441 aactagttgg gccagacgat ctcgatcggc cgtgcgacgt tcccagccga cggaattgaa
     1501 cagcacgctg agttgttgga gatcgatgtc gtgggaatca gaataagtga tcattgggtc
     1561 atcgcgtaag caacgaatca acaacgaatt gttttgcgaa tcattgtttc gctgtcaaat
     1621 cgctgtaacc gttcgggtgc gatcgatgcc agtcccacgc ggtagcaatg atgtcgtgca
     1681 gcgcttcgta gcgcggctgc cagccgagtt cgcgcctgat cttgtcgctg ctggcgatca
     1741 agatcgccgg atcgcccgat cggcgcgggc cgaattccat ttttatttcg cggcccgtga
     1801 tcgatcgggc cgcatcgatc acttctttga ccgaatggcc ttgtccgttg cccagattgt
     1861 aagtgcggct gccttgatcg agcgcgcgca aggcgagaat gtgcgcttga gccagatcgg
     1921 tgacgtgcac gtaatcgcgg atgcaggttc catcgggcgt ggaatagtca ttgccgaaca
     1981 cgctgatctt ctcgcgctga ccaagcgcaa cttgcaggat gagcggaatc aaatgcgtct
     2041 cgtgcgcgtg atgttcgcct cgattgccgc gcggcgaagc accggccgcg ttgaagtaac
     2101 gcagcgcggc atatcgaagt tgagaagttc gatcaagcca atgcaaaatt cgctcgacga
     2161 tatatttcga ttcaccatac gggctgcccg gcacgatgcg ctcggcttca tcgatcggca
     2221 tgcgcgccgg atcgtcgaag agattggcgg tcgacgacag aatgaagcgc ttgacgttgt
     2281 gatcgatcat ggcgcgcagt aagttgagcg cgttagtgac gttatcaccg acgtacaaga
     2341 aaggctgggc catgctttcg ccgacgagtg tgtatgcagc aaagtgcatg acgccatcga
     2401 atgaatgatc atcaaacaat cgctggagta acgcggtgtc tttgagatcg ccttcgatga
     2461 aggtggcttt gggatgcacg gcagcgcgat gcccggtata caggttatcg atcacgacca
     2521 ccgattcacc ggactcgcat aattcttcga cgacgtgact gccaatgtaa cctgctccgc
     2581 ccgtaacgag aatattcaaa agtgactcct taaaaaaatt caatgtgtgc tgcgtaatga
     2641 cgtgagtatt tagcaagcaa gggattttac tatgagccgg taagtaattc cttttacttt
     2701 ttgagatcga ttaaccgaag tcgcccgccc cagaaaacaa ttttgtcacc gctgattgag
     2761 atcgcaaagt catttccaat tgtaccggtc aagtaaggtt cacttaatag cgttgatgta
     2821 atgccagatg caatgctata aacacgaatt tcgtcagtag gtt
//
LOCUS       DASRTJ010000239         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_157263,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000239 DASRTJ010000000
VERSION     DASRTJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157263"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..879
                     /locus_tag="VFF70_05010"
     CDS             31..879
                     /locus_tag="VFF70_05010"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZY44093.1"
                     /translation="MRVRRCLLFMPGDDLKKIQKGISLDVDTIIMDLEDGVALNRKAE
                     ARKTIATALSDLNFERSERLVRINPIGSGLQRNDLDAVLPYHPDGIVIPKVERADQVQ
                     WVSKQITDEHTRLLVLIESARAIINLREIAAADPRIDALIFGSEDFASDIGATRTREG
                     REIFFARSAVVIHTAAFGLQPIDTLVTDFNDEARLIQDCQLGAQLGFVGKLAIHPKQV
                     AIIQAAFTPSDEEIARAQKLIAAYADQQVSGSGAFAIGGQMIDRPIVRAAEQVLAKAR
                     AAGKIS"
     gene            889..>1554
                     /locus_tag="VFF70_05015"
     CDS             889..>1554
                     /locus_tag="VFF70_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44094.1"
                     /translation="MHKFISIFIAASLIVNFYPASASVAQPSNNIQPASAITDPPIDE
                     TEPDPYVCTYAESIGPWCRASSIGNRHDHHLLRHATLGNKRIVAFYGSPLGRGLGVLG
                     NNTPENMLKQLRAQTAEYQNILTGTQVIPAFHMVVTVADKYPGDDKNYNHRVDPAVIQ
                     KWIDWAKQENVWVILDIQPGRSDGITEIDQIEPFLYQPHVQLAIDPEFMVGANDIPGE
                     NLGV"
BASE COUNT          383 a          442 c          403 g          326 t
ORIGIN      
        1 cattactcgt tactcattac ggagtgaatc atgcgtgtcc gtcgttgcct gcttttcatg
       61 cccggcgatg atctaaaaaa gatccaaaaa gggatcagcc ttgacgtcga tacgatcatc
      121 atggatctcg aagacggcgt cgcgctcaat cgcaaggctg aggcacggaa gacaatcgcc
      181 accgcgctga gtgatttaaa cttcgagcgc tcggagcgct tggtgcgcat caacccgatc
      241 gggtcaggct tgcagcgcaa cgatcttgat gccgtgctgc cgtatcatcc cgatggcatc
      301 gtcattccca aagtcgagcg cgccgatcaa gtgcagtggg tcagcaaaca aattaccgac
      361 gaacacactc gcctattagt cctgatcgaa tccgcgcgcg ccatcatcaa cttgcgagag
      421 atcgccgccg ccgatccgcg catcgacgca ttgatcttcg gctcggaaga tttcgccagc
      481 gacatcggtg cgacgcgcac gcgtgaggga cgggaaatct ttttcgcgcg cagcgctgtc
      541 gtgatccata cggcggcctt cggcctgcaa ccgatcgata cactggtcac ggacttcaac
      601 gatgaagcac ggctgatcca agactgccaa cttggcgcgc agctcggctt tgtcggaaaa
      661 ctggcaattc atcccaagca agtggccatt attcaagcag cctttactcc gagtgatgaa
      721 gaaatcgccc gcgcccaaaa attgatcgct gcatacgccg atcagcaagt cagtggatcg
      781 ggcgcatttg cgatcggcgg ccagatgatc gatagaccga tcgtccgtgc ggcggaacaa
      841 gtcttggcga aagcgcgagc ggcaggaaag atcagttaaa cggggatcat gcacaaattt
      901 atttcgatat tcattgcggc tagcttaatt gtaaattttt atccagcttc agcaagtgtc
      961 gcgcaaccat ctaacaacat ccagcccgct tcggctatta ccgatccacc catcgacgaa
     1021 accgagccgg acccctatgt ctgtacatac gccgagtcaa tcggcccgtg gtgtcgcgcc
     1081 tcctcgatcg gcaatcgaca tgatcatcat ttgttacggc atgcgacatt aggcaacaaa
     1141 cgcatcgtcg cattttacgg cagcccgctc ggtcgaggct tgggcgtgtt gggcaataac
     1201 acgcccgaaa atatgttgaa gcaactccgc gcgcaaaccg ccgaatacca aaacatatta
     1261 accggcacgc aagtgatccc cgcgtttcat atggtcgtga cggtcgcgga caagtatccc
     1321 ggcgatgaca agaattacaa tcatcgtgtc gatccagccg tcatccaaaa atggatcgac
     1381 tgggccaagc aagaaaatgt atgggtcatc ctcgacattc aacccggccg cagcgatggg
     1441 ataactgaga tcgaccagat cgaaccgttc ttgtatcagc cgcatgtgca gttggcgatc
     1501 gatccggaat tcatggtcgg cgcgaatgat attcccggcg aaaatctcgg cgtc
//
LOCUS       DASRTJ010000240         5892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_51812,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000240 DASRTJ010000000
VERSION     DASRTJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51812"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1030)
                     /locus_tag="VFF70_05020"
     CDS             complement(182..1030)
                     /locus_tag="VFF70_05020"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZY44095.1"
                     /translation="MSAELQWLSDITFFNLLDEDERAVLSKQVEARRFPAGSVIFKAG
                     DPGNSMYVIRTGSVEISLIDEDTERVVLAVFSDSDFFGELSLLDEEPRSSTAIALKDT
                     DAVLIDREDLRLLFSQKPHAALDMLSVIGKRLRENDKLIRSRAARNANEMIDEQLTFG
                     DRLADWMTSGIGTMKFIYFSAIWFGVWIVLNMGLIKSLAPFDPFPFGLLTMVVSLEAI
                     FLSIFALISQNRMNAKDRVRSELDYNVNLKAELEVAQLHQKLDGVREELLEAIGVILQ
                     HEASSS"
     gene            1229..3367
                     /locus_tag="VFF70_05025"
     CDS             1229..3367
                     /locus_tag="VFF70_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44096.1"
                     /translation="MTIPLDSLVGELNLTGGARQSVTPATGVFTAPRRSARGRDTDTL
                     YVLIDLEGKAPAMLIDEMKQHLARVYWSTPGSVTAALRAAVSSASEWLMTRNVEAASA
                     DRLFGGISCAVLRHAEVFSAQAGLANLYVAHQGSIEQFSSRDADPLVAIGMTRSTDVR
                     FAHAELQPGDVVLLTDARSPKYLPLASISSAIVSVGVEAALRNLEKLTGSGDLIALVV
                     ETVPARDAAAARQEPTSEAASPFSVRATAQKTVAATDSVYSSSRSDAKVQSTRPVDDS
                     TQSYEPRPNPIGRITQTIGHGFNRSLAALATLMQRSLPGPAVSNRSKSKPGFEVSPKI
                     MASIAIGIPIMVAVLVATIYIQGKTQSELQALVVGAQNEMTLAAQANGSEARPHWAAA
                     LDQAQRAVHLVGADKTANELLTQAQTQLDKLDNIVRLTPIVLYNFKSSGPLRLALHNF
                     SIFVLDRSSNELERVTLNTNADGIEGGKPAIVYTSETATDGKKPGNFIDMVWVNATTA
                     QLNSSLMILHRDGLLQYDLTFGFKSLTLGIDAVPSGARRLRSFDGNLYLLDPPAKQVW
                     RYRAKDSAYAAAPETYFDQANADAANAVDMAIDGNVYLISADGRVIKYSQGITNSFKV
                     DGLADPMSTPSIIAVDSEATNSSVYIVDQASQRIVQLRPDGQFVRQFRANGNEFNSIN
                     DLAIDERSSRLYVISAGVLYSVPLPPISSP"
     gene            3437..4816
                     /locus_tag="VFF70_05030"
     CDS             3437..4816
                     /locus_tag="VFF70_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZY44097.1"
                     /translation="MSLNRNVETTHRPPSRLLILWIAGFLVIGVIASFTFFNLVRDLV
                     RDWNVTALAAPNSVGNHDPASGPTKIVIPDWTGIERVNILLLGIDEREQEQGPWRTDT
                     MIVLTIDPATKTAAMLSIPRDLWVQLPVYNTEGKINTAHFLGDIYDYPGGGPALAQAT
                     VQYNLGIPTQYYIRLNFSAFEKLIDLIGGIDVYVDQNIDDPDYPAISGPGFDPFKIEL
                     GQHHLDGATALKYARERHTSGGDFDRARHQQQVILAVRDKITQADMLPTLVGKAGELI
                     DTLGNSVQTDLSLDQLIQLAKLGTQIDLKKIQNLAIDVNMVLNFSTPTDPPQDVLIPI
                     RDEIRKVRDKFLGVVPVAAAATLSETARIRVENGTLSEGLAAKTADHLTGLGFDIIDF
                     TSADFFDYTQTQIINYNNKPQTATALAQALGLSATSIISATGAGANIDIVVILGSDYQ
                     LPSVPTPIP"
     gene            5007..5732
                     /locus_tag="VFF70_05035"
     CDS             5007..5732
                     /locus_tag="VFF70_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44098.1"
                     /translation="MASSNGWDKWYKQWNERFGDQDEAPSFVAQDRLEVWGARFRDLI
                     MLNDHWMGYQHPGSMSIEDGMSIAFVLADSGVEVDELLSLMDEFQPIEGSIVRVGIPL
                     CWLTDPMDHVYDLLSMLSDLPHLDPELIEQMQQTLTEMLGTAANDDDDVQDVFRTMSV
                     NTVQWLLEMMEILGDEGRMSLRAWWQGRLITLLREEEAATKRKRTQRRRKKGQPVPRA
                     FQDLIQSLDMQGMETDTDEDESE"
BASE COUNT         1379 a         1616 c         1612 g         1285 t
ORIGIN      
        1 ggctttgatc ctgttgaacg aaccagctgg cgtcagcgtg ttgatcatcg gcgggctgtt
       61 gtatgcgggc gttacggcct actcatcgat cgtgtggacg atcgcctatc gacgttatgc
      121 gcgttaatac cagtaaatga aacaaataac gagcggcctc aactggtcgc ccgttttttg
      181 tttaacttga acttgcttcg tgctgcaaga tcacgccgat cgcttccaag agctcttcac
      241 gcaccccatc cagtttctga tgcagttgcg ccacttccaa ctccgctttc aagttgacgt
      301 tgtaatccag ttcggaccgt acccgatctt tcgcgttcat ccgattttga ctaatcagtg
      361 caaagatgga caggaaaatc gcttccaaag atacgaccat cgtcaacaaa ccaaaaggaa
      421 acggatcaaa tggtgccaga cttttaatca agcccatgtt cagcacgatc cacacgccga
      481 accaaatcgc gctgaagtag atgaatttca tcgtgccgat gccggacgtc atccaatccg
      541 ccagccgatc accgaaggtc aactgttcat caatcatttc attggcattg cgcgccgcgc
      601 gtgaacggat cagcttatcg ttctcccgca gccgtttgcc gatcaccgac agcatgtcca
      661 gcgcggcgtg cggcttttga ctaaataata accgcagatc ttcgcgatcg atcagcactg
      721 catcggtatc tttcagcgca atggccgtcg acgagcgcgg ctcctcatcc aacaaggata
      781 gttcaccgaa gaaatcgctg tcggaaaaaa cggccagaac cacccgctct gtatcttcat
      841 caatcagcga aatctcaacc gagcctgtgc gaatcacgta catggaattg cccggatcgc
      901 cggctttaaa aattacgctg ccggctggaa agcgccgcgc ttccacttgt ttggacaata
      961 ccgcgcgctc atcttcatca agcaagttaa aaaaagtaat atcagatagc cattgcaatt
     1021 cagcggacat tgcgaagtct ccctttgaat aagtttgcag gtattataag cgggctttac
     1081 ttgaaaagca aaaaatgcag gtattcgcgg ctaaacacga attcagtcca gacgtcaagg
     1141 ctggatatcg cttcaggctc tagcaggttg tgtcgatcaa ccggcccggc gtcgtccttc
     1201 tgactttaat gtgctaaaat aagtcgcgat gactatcccc ttagactccc tagtcggcga
     1261 actcaatttg accggtggcg cacgccaatc ggtaacgcct gccaccggcg tatttaccgc
     1321 gccgcgacga tcggcccgcg ggcgtgacac cgacacgctg tatgtgttaa tcgatctgga
     1381 aggcaaagcg cccgcgatgt tgatcgatga gatgaagcag cacttagcgc gcgtgtattg
     1441 gtcgacgccg ggcagtgtca ccgccgcgct gcgagctgca gtcagctcgg ccagtgaatg
     1501 gctgatgacg cgcaacgtcg aagcggcatc cgccgatcga ttgttcggcg gcatctcgtg
     1561 cgccgtgctg cgccacgccg aagtcttcag cgcgcaagcc ggcttggcaa atctgtatgt
     1621 cgcgcatcaa ggatcgatcg aacagttctc gtcgcgcgat gcggatccat tggtggcgat
     1681 cggcatgacg cgctccaccg atgtacggtt cgcgcacgct gaattgcagc cgggcgatgt
     1741 ggtgttgttg actgatgcgc gctcaccaaa atatctgcct cttgcgtcga tcagcagtgc
     1801 catcgtttcc gtgggcgtag aagctgctct gcggaatttg gaaaaactaa cggggtcagg
     1861 cgatttaatc gcgctcgtcg ttgaaactgt gccagcacgc gatgcggctg cggcgcggca
     1921 agagccaaca tcagaagccg cttcaccgtt ctcagttcgg gcgacagctc aaaaaactgt
     1981 ggcggcgaca gattcagttt attcatccag ccgatcagac gcgaaggttc aatcgacacg
     2041 acccgttgat gattctaccc aatcctatga gccgcgccca aatccgatcg ggcgtatcac
     2101 ccaaacgatc gggcacggat tcaaccgcag tctggccgcg ctcgccacat tgatgcagcg
     2161 cagcttgccc ggcccggcag tctccaatcg atcgaaatcg aaaccgggtt tcgaagtgag
     2221 tccgaaaatt atggcgagca tcgccatcgg cattccgatt atggtcgccg tgctggtggc
     2281 cacgatctat attcaaggca aaacacaatc ggaactgcaa gcgttggtgg ttggagcgca
     2341 gaacgaaatg accttggccg cgcaagcgaa tggctcggaa gcgcgcccgc attgggcggc
     2401 agcactcgat caagcccaac gcgcagtgca tttagtcggc gctgacaaga ccgcgaatga
     2461 attattgacg caggcgcaaa cacagctgga caaactcgac aacattgtgc ggctaacgcc
     2521 gatcgttttg tacaatttca aatcaagcgg gccgctgcgc ttggcgctgc ataatttttc
     2581 gatcttcgtg ctggatcgca gcagtaatga acttgagcgc gtcacgttga atacaaacgc
     2641 cgacgggatt gaaggcggca agcccgccat cgtctacacg tctgaaacgg ccacagatgg
     2701 caagaagccc ggcaacttta tcgatatggt gtgggtgaat gccaccaccg cccagctcaa
     2761 tagcagcttg atgattttgc atcgcgacgg tctgctgcaa tatgatctta cttttggctt
     2821 taagtcgctg actttaggca ttgatgcggt acccagcggc gcgcggcggc tgcgctcgtt
     2881 cgacggaaat ttatatctgc tcgatccccc tgctaaacaa gtctggcgct atcgcgctaa
     2941 agacagcgcg tatgccgctg cgcccgaaac ttatttcgat caagccaacg ccgacgccgc
     3001 caacgcggtc gatatggcga tcgatggcaa cgtgtatttg atcagcgcag acggcagagt
     3061 catcaaatac tcacaaggca taaccaattc attcaaggtc gatggcctag ctgatccaat
     3121 gagtacgccg agcatcatcg cggtggattc agaggcgacg aatagttcag tttatattgt
     3181 cgatcaagct tcgcagcgga tcgtgcagct gcgacccgac ggtcaattcg tgcgccagtt
     3241 tcgcgccaac ggaaacgaat tcaactcgat caatgatctg gccatcgatg aacgcagcag
     3301 ccgcttgtat gtcatcagcg cgggggtgtt gtatagtgtg ccgctgccgc cgataagtag
     3361 tccttaacaa atgtctaact cgctgaagaa tcgatcgtcg atcgactgag ccaacttcag
     3421 tggatagatc ttctgtatgt ctttgaaccg gaacgttgaa acaactcacc gaccgcccag
     3481 ccgcctgctg atcctatgga tcgccggctt tttggtgatt ggcgtgatcg cctcgttcac
     3541 gttcttcaat ctggtgcgcg atctcgtccg cgattggaat gtgaccgccc tcgccgcgcc
     3601 gaacagcgtg ggcaatcacg atcccgccag cggaccaacc aagatcgtca taccggattg
     3661 gaccggcatc gagcgcgtca acattttgtt gttgggcatc gacgaacgcg aacaggaaca
     3721 aggcccgtgg cgcacggata cgatgatcgt gttgacgatc gatccggcga cgaagaccgc
     3781 ggccatgctg tcgatcccgc gcgacttgtg ggtacagctg ccggtctata acaccgaagg
     3841 caaaattaac accgcgcatt tcctgggcga tatctacgat taccccggcg gcggaccggc
     3901 gctggcgcaa gcgactgtgc aatacaatct gggcatcccg acgcaatact acatccggct
     3961 gaatttcagc gcgttcgaaa aattgatcga tctgatcggc ggcatcgatg tctatgtcga
     4021 tcaaaacatc gacgatccgg attacccggc cattagcggt cctggctttg atccatttaa
     4081 gatcgagctg ggccagcacc acttggacgg cgctacggct ctgaaatatg cgcgtgagcg
     4141 tcacacgtcg ggcggcgatt tcgatcgggc gcggcatcaa cagcaagtga tactggctgt
     4201 gcgcgacaag atcacccagg ccgatatgct gcccacgctc gtcggcaagg ccggtgagtt
     4261 gatcgataca ctgggcaatt ccgttcagac tgatctgtcg ctcgatcagt tgatccagct
     4321 cgccaagctc ggcactcaga tcgatctaaa gaagatccag aatttagcca tcgacgtgaa
     4381 catggtgctg aatttcagca cgccgaccga tccgccgcag gatgtattga ttccgatccg
     4441 tgacgagatt cgcaaggtgc gcgataaatt tttaggcgtg gtgccggtgg ccgctgccgc
     4501 taccctgagt gaaacggccc ggatccgcgt ggagaatggt acacttagcg aaggactcgc
     4561 agccaaaaca gccgaccatt tgaccgggct gggtttcgac atcatcgact ttacttcggc
     4621 tgattttttt gattatacgc agacacagat cattaactac aacaacaagc cgcagacggc
     4681 cacggcactc gcccaggctt tgggcttgtc ggcaacctcg atcatttcgg cgaccggcgc
     4741 gggcgcaaac atcgacatcg tcgtcattct cggcagtgat taccagctgc cgtcggtgcc
     4801 aacaccgata ccgtgagaac gttccgtcat tgcgaggcgc gcacagcgcg acgaagcaat
     4861 ctcccgatcg agttgatcaa tcccgatgag acacctgcac tgctccgcag cgcggtgcaa
     4921 gtgttgccac gccgcccccg aaggccctgc ccgatgtttg cagggttggc ggctcgcaat
     4981 gacgtcgaaa caaagtaggg agacacatgg cttcatccaa cggttgggac aaatggtata
     5041 agcagtggaa cgaacgcttc ggtgatcaag atgaagcgcc cagtttcgtg gctcaggatc
     5101 gactggaagt ctggggcgcg cgctttcgcg acttgatcat gctcaacgat cactggatgg
     5161 gctaccagca tcccggctcg atgtcgattg aagatggcat gtcgatcgcg tttgtgctgg
     5221 ccgattccgg cgtcgaagtc gatgaactgc ttagcttgat ggatgagttc cagccgatcg
     5281 aaggttcgat cgtgcgggtg ggcattccct tgtgctggct aaccgatccg atggatcacg
     5341 tttacgatct cttgagcatg ttgtccgatc tgccgcatct cgatccggaa ttgatcgagc
     5401 agatgcagca gacgctgacg gaaatgctgg gcacggctgc gaatgacgat gacgatgtgc
     5461 aggatgtctt ccgcacgatg tcggttaaca cggtgcaatg gttgttggaa atgatggaga
     5521 ttctgggcga tgaaggccgc atgagtctgc gcgcgtggtg gcaaggccgc ttgatcacgc
     5581 tgctgcgcga agaagaggcc gcaacgaaac gcaagcgcac tcagcggcgg cgcaaaaaag
     5641 gtcagccggt gccgcgcgcc ttccaggatt tgatccagag tctggatatg cagggcatgg
     5701 agacggatac ggacgaagat gaaagtgagt aaacagttaa tcagagatta ggaattagga
     5761 aatggggaaa ctccggctcg tttgagccgg agttttttgt tctgagctct agacgttgac
     5821 aacagatgaa ctaaagtcaa atgcaatttt ggaatgcgga aggcggattc accctcacct
     5881 caattaacat tc
//
LOCUS       DASRTJ010000241         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_240792,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000241 DASRTJ010000000
VERSION     DASRTJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240792"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /locus_tag="VFF70_05040"
     CDS             <1..1643
                     /locus_tag="VFF70_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZY44099.1"
                     /translation="IQLHRSFSYQYNLAVTLNDYAEIMQARGQMTRAIQVQREALEIW
                     RQRGTPAPLAIALHHIAQGMHMLGQYQVALATYAEALNWAERSGNPRWEAIVLAGQGD
                     LLADIGDVHSALDLFRQAMLKAEQCHDRNIVIPLYRSIARCERWLHNFTDALKWVSAA
                     EAESLQASEVSKLDLGGLHGILLVEIGSRAEGRKILEGACSVLERADRPADLAQTLLF
                     RGYAEFLEGDQPAAIVSLEQSFRLADSLGYEQMLLSEALSVREMLTVLSSNAHIGRRV
                     TDLISHAEAARSSQKHIQPDTIAPPGNQNSALTVRALGQTRVMKNNVEIQRTAWASQK
                     TRELFLFLVDRSPIARDEVLAVFWPDMTTSRAVSNLHQTLYRLRRAVADDLILTDDLV
                     CRISGTIDLQYDVAHFEEQAKGALGLRPHDPRRVELLASAANLYTGEYLADLSVDWLM
                     PRREELSALQIQVLREYADELMALTRYSEARSVLAEALAVEPFQDELHERMLICLGKI
                     GQRHEVVNYYRRYRETLRNELGIEPPHQIRALYSRLLE"
BASE COUNT          461 a          478 c          526 g          517 t
ORIGIN      
        1 cgattcagct gcatcggtct ttctcctatc aatacaatct cgccgtgacg cttaatgatt
       61 atgcagaaat catgcaagcg cgcggtcaaa tgactcgggc gatccaggtt caacgcgagg
      121 cgctagagat ctggcggcag cgcggcactc ccgcgccact ggcgattgca ctacatcata
      181 ttgcccaagg tatgcatatg ttgggccagt atcaagtggc gctggctaca tatgctgaag
      241 ctctcaactg ggcagagcgc agcggcaatc ctaggtggga agcaattgtt ttagcgggcc
      301 aaggggattt gctggcagac atcggcgacg ttcattccgc tttggatctt tttcgacagg
      361 cgatgcttaa agcagaacaa tgtcacgatc gcaacatcgt gatacctctc taccggtcga
      421 ttgcgcgctg tgaaagatgg ttacacaatt tcacggatgc attgaagtgg gttagtgctg
      481 cggaagcaga atccctgcaa gcttctgaag tcagtaagtt agatctgggt ggattacatg
      541 gaatcctatt ggtcgagatt ggctcacggg cagaagggcg taagatcttg gaaggcgctt
      601 gctcggtact cgaacgtgcc gatcggccgg ccgatttggc gcagacgctg ctctttcgcg
      661 gatatgccga attcctcgaa ggcgatcaac cagcggcgat cgtttcattg gagcaatctt
      721 ttcgcctggc tgatagtttg ggttatgagc aaatgttatt gagcgaagca ctttctgtac
      781 gtgagatgtt aaccgttctg tcgtctaatg cgcacattgg ccggcgcgtg acggatctga
      841 tttcgcatgc cgaagctgca cgatcgtctc aaaaacatat ccagccggac actatcgcac
      901 cgcccggcaa tcaaaactcc gctttgactg tccgcgcatt aggacagact cgagtcatga
      961 aaaataatgt cgaaattcag cggacagcct gggcttcgca aaagacgcgc gagttatttc
     1021 tgttcttggt cgatcgatca cctattgcgc gcgatgaagt gctggcagtc ttttggcccg
     1081 atatgacgac atctcgagcc gtgtccaatt tacatcagac actttatcga ctgcgccgag
     1141 cggttgctga cgatcttatc ctcaccgacg atctggtttg ccgcatctcc ggcactattg
     1201 atttacagta tgacgtggca cactttgaag aacaggccaa gggcgctctg ggcttgcgac
     1261 ctcatgatcc tcggcgcgtc gaattgttag caagtgctgc caatctgtat accggcgaat
     1321 atttggctga tttatcggtg gattggttga tgccgcgccg tgaagaacta agcgcgctgc
     1381 aaattcaagt tttacgcgaa tatgccgacg agttgatggc actcacgcgt tactcagagg
     1441 ctcgttccgt tttggcggag gcattagcgg tcgagccgtt tcaggatgaa ctgcatgagc
     1501 ggatgttaat ttgtctggga aaaatcggcc agcgccacga ggtggtgaat tattatcgtc
     1561 gttatcgcga gacgctgaga aatgaattag gtattgaacc tcctcaccag atccgggcgt
     1621 tatattcgcg gctccttgaa tagtcgataa acgaatttga aaatcgaatt aattatgaac
     1681 aggtgagcca aatttggttc acctgttgtt atgtctgttg atagcttcgt tgataagagt
     1741 tgatgtatag ttggcctaat ctcaatactc aggagcacat aatccatgtc aacaaacact
     1801 ttggtcaact ttcattctac gatcgtcaag tggttgtcca tcaacccgca tttgtggcga
     1861 gcaatgatta tcataaccat cgtgctggta tttgccttac tggcgcccag catggcgtat
     1921 gctcatcctg cactcagcgg gtgtggcgcc cccggttgct agcaacgatg tcgtctatac
     1981 ac
//
LOCUS       DASRTJ010000242         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_316625,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000242 DASRTJ010000000
VERSION     DASRTJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316625"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1408
                     /locus_tag="VFF70_05045"
     CDS             164..1408
                     /locus_tag="VFF70_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44100.1"
                     /translation="MTTTSRRVIDSWVWLLGLYSAASFIEAAFWGQMGSFTPLYLPKL
                     GIAPADVKSWTGIIVAISGLVGLPFLPLWGALADRYSRQPIIVRSFVAHLIAGIISIL
                     AGNVWVFLLGRSIMSFSLGNSGLMMTTLSERAPKHRIGFAFSIMNSAAPIGAFVGPLF
                     GGPIVDAHGFPTLLIIDTVLMLIVILALTFGYRDHFKGTSRESLPRMMADSVKLIWSS
                     RRLRTLFPALFMLFAGWMLAFTYVPIAVTELYHGTDPNSAVGLVAGAGGFVTLILSPI
                     IGALADRHGHWRLLFIGSIVAIILWPIPGFMRELVPFAIAWAILNGVVSSVFALSFSV
                     LSSSAPSSVRGRIMSFAYLPVNVGYIIGPTIGSIVTQVSTFAVFPVAAVFTAIGLGGL
                     VLAYKQRMPTEVTTNAATSAKS"
     gene            1487..2863
                     /locus_tag="VFF70_05050"
     CDS             1487..2863
                     /locus_tag="VFF70_05050"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HZY44101.1"
                     /translation="MKRALSLAAIFVCMVIVSIGSANRSADAAISSIFTVNSTADVSD
                     VNPGNNVCETAVSNHICTLRAAIQEANAHSGADTISIPSGTYQLTQIAHDGFITAGLI
                     ITTPITILGASANNTIIDGNAAVLHARVFNITGTVTISGVTIQHGHSYNTAGGIYNTG
                     QLIIINSAIISNTANGLNDWGGGIYNAGPMTLTNSLIRGNTTGNHNAYGGGMYNQGPL
                     LVIDSTFSDNSTSAGTSSPGEGGGLFSIGYTTTIKTSTFSGNSAALGGGIYHGGYALY
                     LINSTISGNNSDGGGGGIYNASGTIDLFNVTVVGNLANADNSGTAIGGGVDNNSGTFN
                     FRNSIIALNGKVVVPGPIYVYSDCAGTITTQGYNIMKDTSDCIVAPVTIADPLLGPLQ
                     DNGGRTQTYALLSNSPAINAGESPYCTDALGAPITTDQRGVTRPINSCDIGAYEYRWT
                     MYLPLVKK"
BASE COUNT          679 a          876 c          832 g          717 t
ORIGIN      
        1 gtattgtcac acccgcggag gcgggtgtcc agcaatttca ttctggattc ccgctttcgc
       61 gggaatgact aggaacattt gctgcaaaga acaaatagaa ttttcacagc agcatttgaa
      121 ttaggtaata actagaagac taacggcttt aatgagagag agcatgacta ccacctcgcg
      181 acgcgtaatc gattcatggg tttggctgct gggtctgtat tcggcggcga gtttcatcga
      241 agcagccttc tggggacaga tgggcagctt cacgccgttg tatctgccca aactcggcat
      301 cgcgcccgcc gatgttaaat cgtggacggg catcatcgtc gccatctccg gcctcgtcgg
      361 ccttccgttt ctgcctctgt ggggcgcgtt agccgatcgg tattcgcgcc aaccgatcat
      421 cgtgcgatcg ttcgtcgcgc atttgatcgc cgggatcatt tcgatcttgg ctggcaatgt
      481 gtgggtgttc ttgctgggcc gatcgatcat gtcgttctca ttgggcaaca gcggcttgat
      541 gatgacgacg ctcagcgagc gcgcgcccaa acaccggatc ggcttcgcgt tctcgatcat
      601 gaacagcgcc gcgccgatcg gtgcgttcgt cgggccgttg ttcggcgggc cgatcgtcga
      661 tgcgcacggc tttccaacgc tgctcatcat cgacaccgtg ctcatgttga tcgtcatcct
      721 ggcgttgacg ttcggctacc gcgatcactt caaaggcact tcgcgcgaat cgctgccgcg
      781 catgatggcc gattcagtca aattgatctg gagttcgcgg cgactgcgca cgcttttccc
      841 ggcgttgttc atgttgttcg ccggttggat gttggccttc acgtacgtgc cgatcgccgt
      901 caccgagttg tatcacggca ctgatcccaa cagcgcggtc ggactcgtgg ccggtgcggg
      961 cggattcgtc acattgatct tgagcccgat catcggcgca ctggccgatc gacacgggca
     1021 ctggcgcctg ttgttcattg gatcgatcgt ggcgattatc ttgtggccga ttccgggctt
     1081 catgcgcgag ttggttccgt ttgcgatcgc ttgggcaatc ctcaacggcg tggtgtcgag
     1141 tgtgttcgcg ttgtcgttca gcgtattatc aagttcagcg ccgagcagcg tgcgcggacg
     1201 catcatgtcc ttcgcctacc tgccggtgaa tgtcggctac atcatcggcc cgacgatcgg
     1261 cagtatcgtc acacaggtca gcaccttcgc agtgttcccg gtcgccgccg tcttcacggc
     1321 aatcggtctg ggtggattgg tgttggctta taagcagcgc atgccgactg aagtcaccac
     1381 caacgctgcc acatcggcta aatcataacc ctgcccatga ttggtcaaaa gctgcgataa
     1441 taatagcatc taattgatgt gattgattgg ctttgggagg ctgaccatga aacgcgcatt
     1501 gtctttagcg gctatctttg tctgtatggt gatcgtcagt atcggctcgg ccaaccgatc
     1561 agctgacgcg gcgatctcgt ccatcttcac ggtcaacagc acggctgatg tgtcagatgt
     1621 caaccccggc aacaatgtat gcgagacggc cgtcagcaat cacatctgca cgctgcgtgc
     1681 ggccatccaa gaagctaatg cccactccgg cgctgatacg atcagcattc caagcggaac
     1741 ctatcagttg actcaaatag cacacgatgg attcatcact gccggcctga tcattacgac
     1801 cccgataacg atcctcggcg caagcgcaaa caacacgatc atcgacggca atgcggcagt
     1861 gctccatgcg cgtgtgttca acatcactgg cacggtcacg atttccggcg tcaccattca
     1921 acatggccat tcctacaaca cagccggcgg tatttacaat actggtcaat tgatcattat
     1981 caacagtgcc atcattagca atactgccaa cggcttaaac gattggggtg gcggcattta
     2041 caatgccggg ccaatgacat tgacgaacag cctcatccgg ggcaacacca ctggcaacca
     2101 caatgcctac ggaggaggca tgtacaatca aggtccgcta ttggtgatcg acagcacttt
     2161 tagtgacaac agcacctccg ctggaacaag cagtcctggt gaaggcggtg gcctattcag
     2221 tattggctat accacaacca ttaaaaccag cactttcagc ggcaattccg ctgcgctcgg
     2281 tggcggcatt taccatggtg gctacgcact ctacctcatc aacagcacaa tatcaggcaa
     2341 caatagcgac ggcggtggcg gcggcatata caatgcatcc ggtacaatcg atctattcaa
     2401 tgttacggtt gtcggcaatc tcgccaacgc ggataatagc ggcaccgcga ttggcggtgg
     2461 agtcgacaat aacagcggca cctttaactt tagaaatagc atcatcgctc tgaatggcaa
     2521 ggttgttgtt cccggtccca tctatgtata cagcgattgt gccggaacga ttacgaccca
     2581 aggctacaac atcatgaagg atacaagcga ttgcatcgta gcccctgtta caatcgctga
     2641 tccccttctc ggcccgctgc aagataacgg cggccgcaca cagacgtacg ctctgctctc
     2701 caatagcccg gcgatcaatg cgggtgaatc gccctattgc actgatgcgc tcggcgcacc
     2761 gatcaccacc gatcagcgcg gagtaacacg gccgatcaat agctgtgaca tcggcgcgta
     2821 tgaatatcga tggacaatgt atttgccgct cgtcaagaaa tagatgaacg aagtaacccg
     2881 caagtacaag tatatataga tgtaagtcga ctgggaggca atgacatgaa cacacgcatc
     2941 gtccgctttc tcttcggcgc aacaatcgcg ttggcgattg cattgttgct gcacgcgcgc
     3001 acgctgcccg tgcaggctag caacgtggtc gtcggtgacg gcgtgaacgc tgccagctgc
     3061 aacatgttca ccttgggcgc ggcgatgaat aacaatacca ccgc
//
LOCUS       DASRTJ010000243         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_316675,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000243 DASRTJ010000000
VERSION     DASRTJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316675"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFF70_05055"
     CDS             <1..787
                     /locus_tag="VFF70_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HZY44102.1"
                     /translation="GSRVIREMPLEIVLKHLDIDELYRLQWGAKNTHGDDWTKLKAEF
                     DARLDRMKREAIKSGYLKPQAVYGYFPAQSNGEALIVYDPNLNASSNGQKSLKEIARF
                     EFPRQSSQDHLCLADYFAPIESGVIDTVALQVVTVGRGADEKFNQLQSKNDYSEAYFL
                     HGLGVEAAEATAEYVHQHIRSELNIGAKQGKRYSWGYPACPDLADHAKVFALLPQTSE
                     LGLELSTAYQLIPEQSTAAIVIHHPAAKYYAVGVNRVEQLMAE"
     gene            849..1193
                     /locus_tag="VFF70_05060"
     CDS             849..1193
                     /locus_tag="VFF70_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZY44103.1"
                     /translation="MAENMIRMQVYLPRGTYERLQKRAKEHDLTLALQIREALESYLE
                     QIEAKQDDGVLRADDPIFKMIGMFDSGLGDLSVNHDHYLYGWPKREEVEAMHEKPAKR
                     YKTKKTAKRKAK"
     gene            1190..1597
                     /locus_tag="VFF70_05065"
     CDS             1190..1597
                     /locus_tag="VFF70_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZY44104.1"
                     /translation="MKLLADSAAWLALYDRGDKFHTQAAAAFHDLSNRKVTFAVNDYL
                     FAETVTLVLGRAGHAQAMVCGDWLLNSPRVHFIRVNIDWWNEAWSTFKLYDDKEFSFV
                     DCLSFIMMRREHIADAFTFDHHFEQMGFRLWPK"
     gene            1748..2497
                     /locus_tag="VFF70_05070"
     CDS             1748..2497
                     /locus_tag="VFF70_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZY44105.1"
                     /translation="MAKFDSNWVPKTAMTIFAHPDDMEFSCGATLAKWAKAGCEITVV
                     LCTSGNVGTHDDVKYTKLSLTETREREQRNAITHLGVQHIEFLQHNDCQLVADLKLRK
                     ELTGLIRTHKPEVVICGDPNMVFWGDNRINHPDHRAAATAAIDAVFPCCEMELLWPKL
                     GKAHKVHAVYVRAFEDLNTWIDVSDTVELKIKALREHHSQLDEMTELEKMIHDRLADA
                     GKEKDMAFAEAFRVIRLIEDQEASQVAQDKK"
     gene            2507..4387
                     /locus_tag="VFF70_05075"
     CDS             2507..4387
                     /locus_tag="VFF70_05075"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HZY44106.1"
                     /translation="MNTEFLARLKSGPILCDGAMGTQIYARGISFEHCFDELNLSQPE
                     AILDIHRRYIEAGAEIIETNTFGGNRFRLIEHGLDNKIAEINQAGVKLARQAANESRR
                     TVFVAGSVGPLGVRLAPLGRVNPADAQQAFKEQIAALNAAGVDLLIIETFSDLNEIEQ
                     AIKAAREVNPDLPIVAQMTFTEDGFTPLGESPDQVAHRLIDLNVDVFGANCSVGPARL
                     LPVIQTLTRHAQISDRKPFVSIQPNAGWPQRIGPRLMYPATPDYFGDYTRRFLEAGAT
                     IIGGCCGTTPNHIRSMRAAIDGAPSHLITIDLRTPADQAAPDRSGEATKIAPTGLAQK
                     FADKKFVTAVEVDPPRGHNPSRIIEAARELKHAGFDVINVADTPLARMRMSAWALAHL
                     IQREADLETVLHFPTRGRNLIRVQGDLLAAHALGIRNLLAVMGDPSSVGDYPEASDQH
                     DIVPSGLVKMIKQHMNTGTDHSGTSIGSATSFHVAVAVNFNAADLDREIKTFRRKILA
                     GADFAISQPIFEVGCVRAFFTRYHELYGRIPIPIMGGVLPPASVRNAEFLHNELPGMH
                     LPDWALDRMRQAVDGRQEGIKIAQEALLQLREWLQGVYVMPMLGRYDSAGEVIEVVKD
                     KA"
BASE COUNT         1094 a         1158 c         1307 g         1002 t
ORIGIN      
        1 gggttcgcgt gtgatccgcg agatgccgct agagattgtg ttgaagcatc tggacatcga
       61 tgaactctat cgcttgcagt ggggcgcgaa gaatacgcac ggcgacgact ggacgaagtt
      121 gaaagccgag ttcgatgctc gactcgatcg aatgaagcgt gaagcgatca aaagcggtta
      181 cttgaagccg caagccgtgt atggttactt ccccgctcaa tcgaatggcg aggccctgat
      241 tgtttatgat ccgaacctca atgcatctag caacgggcaa aaatcgctga aagagatcgc
      301 gcgtttcgag ttcccacggc aatctagcca agatcatttg tgtttagcgg attacttcgc
      361 gccgatcgag tcgggcgtga tcgataccgt tgcattgcaa gttgttaccg tcggtcgcgg
      421 cgcagatgaa aaatttaatc agttgcaaag caagaatgat tactcggaag cgtatttcct
      481 gcacggtctc ggcgtcgaag cggctgaagc caccgccgag tatgtgcacc aacacattcg
      541 cagcgagttg aacatcggcg cgaagcaggg caagcgttac tcgtggggtt atcccgcttg
      601 tcccgatctc gccgatcacg cgaaagtgtt tgccttgctg ccgcaaacat cggagttggg
      661 cctggaactt tcgacggcgt atcaactgat tcccgagcaa tcgaccgccg cgatcgtgat
      721 ccatcatccg gcggcgaaat attacgcagt cggcgtgaat cgcgtcgagc agttgatggc
      781 cgaatagatg tatacaacat acatctattg atgtataata catacatcga tgaagtaaga
      841 ggtgcagcat ggccgagaat atgatacgca tgcaagtcta cctgccgcgc ggtacatatg
      901 aacgcctgca gaagcgtgca aaggaacacg atttaacact cgctttgcaa attcgggaag
      961 cgctagaatc ttatttggag caaatagagg cgaaacaaga tgatggcgtt ctccgtgctg
     1021 acgatccgat ctttaagatg atcggtatgt ttgacagcgg tctgggggat ctatcggtca
     1081 atcacgatca ttatttgtat ggctggccca agcgcgaaga ggttgaggcc atgcacgaaa
     1141 aacctgctaa gcgctataag accaagaaga cggcaaagcg aaaagcaaaa tgaaattact
     1201 tgccgatagc gcggcctggc tagcgttata cgatcgcggc gacaaatttc acacacaggc
     1261 tgccgctgcc tttcatgatc tatccaatcg aaaagtcact tttgccgtaa atgactattt
     1321 gttcgctgaa acggtcacgc tggttttagg tcgggcagga catgcccaag ctatggtttg
     1381 tggcgattgg ctgctcaact cgccacgcgt gcattttata cgagtcaaca tcgattggtg
     1441 gaatgaagcg tggtcgacgt tcaaattata cgatgacaag gaattttcct ttgtcgattg
     1501 ccttagtttc attatgatgc ggcgcgaaca tattgcggat gccttcactt tcgatcatca
     1561 cttcgagcaa atgggctttc gcttgtggcc caagtaagta cgatagcaag aatgcgagac
     1621 ggcgcgaaat gcgatatgcg ataagctatc tgctatccgc catctgctaa atcagaaaca
     1681 cctgcactgc atccgcagcg cggtgcaagt gtcaaacatc agagatcaga gataccaagg
     1741 ggagaccatg gccaagttcg atagcaattg ggttccgaaa acggcaatga ccatcttcgc
     1801 ccatccggac gatatggaat tttcctgcgg ggcgactttg gcgaagtggg ccaaagcggg
     1861 ttgcgagatc acggtggtgt tgtgcaccag cggcaacgtc ggcacgcacg atgacgtgaa
     1921 atataccaag ctctcattaa ctgagacccg cgagcgcgaa caacgtaatg cgattacgca
     1981 tctgggcgtc cagcatattg aattcttgca gcacaatgat tgccagctcg tggccgatct
     2041 gaaactgcgc aaagaattga ccggcttgat ccgcacacac aaacccgaag tggtgatttg
     2101 cggcgatccg aatatggtct tctggggcga caatcgcatc aatcatcccg atcatcgcgc
     2161 ggcggcgacg gcggccatcg atgcagtttt tccgtgctgc gaaatggaat tgttgtggcc
     2221 gaagctgggc aaggcgcata aagtgcatgc tgtctatgtg cgcgcgtttg aagacctgaa
     2281 cacctggatc gatgtcagcg ataccgtcga gttgaagatc aaggcgctgc gcgagcatca
     2341 cagccagtta gacgagatga ccgaactcga aaagatgatc cacgatcggt tggcggatgc
     2401 gggcaaagaa aaagacatgg cgttcgcgga agcgttcaga gtgattcgtt tgattgaaga
     2461 tcaagaagcc agccaagtcg cgcaagacaa gaaatgagac cgcgttttga atactgagtt
     2521 tctggcaaga ttaaaatccg gacctatctt gtgtgacggg gcgatgggca cgcagattta
     2581 cgcgcgcggc atttcgttcg aacactgctt cgacgaattg aatctctcgc agcctgaagc
     2641 gatcctcgat attcaccggc gctacatcga agcgggcgcg gagatcatcg agacgaacac
     2701 gttcggcggc aatcgctttc gcttgatcga gcacggactc gataacaaga ttgccgagat
     2761 caatcaagcg ggggtgaagt tggcgcggca ggcggcgaat gaatcgcgcc gcaccgtttt
     2821 tgtcgcaggc tcggtcgggc cgctgggtgt gcgcctcgcg ccgttgggcc gcgtgaaccc
     2881 agcggacgcg cagcaagcct tcaaggaaca gatcgcggcg ctcaatgcgg cgggcgtcga
     2941 tctgttgatt atcgaaactt tcagcgactt gaatgagatc gagcaagcga tcaaagccgc
     3001 gcgtgaagtg aatcccgatc tgccgatcgt cgcgcagatg acgttcactg aagatggctt
     3061 cacgccgttg ggtgaatcgc ccgatcaagt cgcgcacaga ttaatcgatc tcaacgtcga
     3121 tgtgttcggc gcaaattgca gcgttggccc ggcacgcttg ctgcccgtca ttcaaacgct
     3181 gacccgccac gcgcaaattt ctgatcgcaa accgttcgtg tcgattcagc cgaatgccgg
     3241 ttggccgcag cgcatcggcc cgcgcttgat gtatcccgcc acgccagatt atttcggtga
     3301 ttatacgcgg cgtttcctcg aagcgggcgc gacgatcatc ggcggctgct gcggcaccac
     3361 gccgaaccat attcgatcga tgcgcgcggc gatcgatggc gcgccctcgc acttaatcac
     3421 gatcgatctg cgcacgcctg cagatcaggc tgcgcccgat cgatccggcg aggcgactaa
     3481 gatcgcacct accggattgg cgcagaaatt tgccgacaaa aaattcgtca ccgcggtcga
     3541 agtcgatccg ccgcgcgggc ataatccgag tcgcatcatc gaagcggcgc gcgagttgaa
     3601 acatgcgggc ttcgatgtga tcaacgtggc tgatacgccg ctcgctcgca tgcgcatgag
     3661 cgcgtgggcg ttggcgcatc tcattcagcg cgaagccgat ctggaaacgg tgctgcattt
     3721 tccgacgcgc ggcagaaact tgatccgcgt gcaaggggat ttgctggctg cacacgcgct
     3781 aggcattcgc aacctgttgg cggtgatggg cgatccctca tcggtgggcg attatcccga
     3841 agccagcgat cagcacgaca tcgtgccatc gggtctggtc aagatgatca agcagcacat
     3901 gaataccggc accgatcatt ccggcacgtc gatcggatcg gctacctcgt ttcatgtggc
     3961 agtggccgtg aactttaacg ccgccgatct cgatcgggaa atcaaaacat tccgcaggaa
     4021 aattttagcg ggcgcggatt ttgcgatctc gcaaccgatt ttcgaagtgg gttgcgtccg
     4081 agcgttcttc acgcgttatc atgaactgta tggccgcatt ccgattccga tcatgggcgg
     4141 tgtcctgccg cctgcgtcgg tgcgcaacgc cgaatttctg cacaacgaac tgccgggcat
     4201 gcacctgccg gactgggcgc tcgatcggat gcgccaggcg gtcgatggac ggcaggaagg
     4261 catcaagatc gcgcaggaag cgctgctgca attgcgcgaa tggttgcaag gcgtgtacgt
     4321 gatgccgatg ctgggccgct atgattcggc gggcgaagtg attgaagtgg tgaaagataa
     4381 ggcatgaggg ataagggctg agggatgaat aagccgccga atggtcaacc atcattcggc
     4441 ggtttttgtt ttgactagca cttacgcagt tttcgaattg tgatttatcg caattttctc
     4501 gacgatttca aaaagctttg tgaaagtaga aaaatcaaaa tcttttgcat gtccatatat
     4561 g
//
LOCUS       DASRTJ010000244         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_316688,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000244 DASRTJ010000000
VERSION     DASRTJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316688"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFF70_05080"
     CDS             complement(<1..875)
                     /locus_tag="VFF70_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY44107.1"
                     /translation="MSDQKRILVTGATGKVGRSFIKHLLSDRNFDTFVVRALCHQREL
                     EANDRVEIVRGSIEHRQTVENALNGVTHVLHLATCKETPDEIMNVAVKGLFWLLETCR
                     TSPTFQQFVLIGGDAGVGHFVYPHPMPVTETQKHSAYPGCYALSKVLEEVMLEQYYLQ
                     YNLNGCCLRAPWIMEKDDFKYQLSFGEDVFGGPRWRDLVSTQQADEYVRTQTIPCMLD
                     PNGQPVKRNFVHLDDLVDAILLALDHPRARQQTFNLCMDEPVDYGQVSAYLASSRGLP
                     SVEIKTPYYSTWLDNT"
     gene            complement(922..2505)
                     /gene="ftcD"
                     /locus_tag="VFF70_05085"
     CDS             complement(922..2505)
                     /gene="ftcD"
                     /locus_tag="VFF70_05085"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZY44108.1"
                     /translation="MPKIVECVPNFSEGRRLEVVAQIRSAIAAIEGVTFLDQHSDPDH
                     NRTVMTFVGDPKAVEQAAFAGIAVAAKLIDLDHHTGQHPRLGATDVVPFVPVSGVSLE
                     ECVAIAKRLGQRVGAELNIPVYLYEAAATRPDRENLENIRRGQYEGIKAEIETDPNRK
                     PDFGPSHLGPAGATVIGARPFLVAFNCYLNTEEVNVAKNIAKVVRNSGGGLKFVKALG
                     LLVDGKAQVSMNLTDYRKTSIARVVEMVRREAARYGHTIVKSELVGLTPQEALIDAAQ
                     WYLQIDNFESHQILENKLAEISSGAAKSIVPNAFLEATAAGSATPGGGAVAALAGALS
                     AGLTGMVARLTIGKKKYAEVDAAMKDIAARADQLRSQLTQAIDADSAAFEEVMAAMKS
                     PKETEAEKSVRTAQIQSATQHATDVPLNAARACLEILSLVHEVAAHGNVNALSDAASA
                     AFMARAAIDAAGMNVRINAASLDDKAAAKRSVEELYSIQSQAADRIEKILDEVGQRAT
                     LAETVKLVELDIGKREEQE"
     gene            complement(2606..3313)
                     /locus_tag="VFF70_05090"
     CDS             complement(2606..3313)
                     /locus_tag="VFF70_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44109.1"
                     /translation="MLELRNVHSYYGSIHALKGISFTVNDGEVVTLIGANGAGKSTTL
                     RTIQGLIRPREGTIILDGEPLEKLPTHEIVKRGVSQSPEGRMIFPQMTVMENLEMGAY
                     SRADKSGVQSDLERVLVLFPRLKERLKQRGGTLSGGEQQMLAMGRALMAKPRIMLLDE
                     PSMGLAPILVEQIFDIVREINQQGTTVLLVEQNATMALQVANRGYVMEVGQIALSGTA
                     EELRNNQQVQDTYLGVQ"
     gene            complement(3315..4088)
                     /locus_tag="VFF70_05095"
     CDS             complement(3315..4088)
                     /locus_tag="VFF70_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44110.1"
                     /translation="MSFLVAHGVTKTFGGLTAVSKVDFVIEQGMIAGLIGPNGAGKTT
                     FFNMITGVYSVTTGALTFNDTNIGALKPHDITALGIARTFQNIRLFPAMTVLENVLVG
                     MHTRLHSTLLDTLLKTDRMTKEEDMARGKALDMLDYVGLGRQRADWTARHLPYGDQRR
                     LEIARALASEPKLLLLDEPTAGMNRAETADLTTFIQFIRKDLSLTVLLIEHHMRVVMG
                     ISDRVSVLDHGEKIAEGTPVEVQKNQQVIEAYLGSGAGH"
     gene            complement(4085..>4411)
                     /locus_tag="VFF70_05100"
     CDS             complement(4085..>4411)
                     /locus_tag="VFF70_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44111.1"
                     /translation="IFGALIILLNDGLILKAFQNLLIGLQTHVLLPATVGNPDLQQFI
                     QANLDPVKYRFLMLGLVLVVVMAVRPEGLFPSRERAEELHASEDDKIEEIEAKLKATP
                     DKVESS"
BASE COUNT          958 a         1211 c         1178 g         1064 t
ORIGIN      
        1 gtattgtcca gccaggtcga gtagtacggc gtcttgattt caaccgaggg caggccgcga
       61 cttgaagcca agtatgcgct gacttgtcca tagtccaccg gttcgtccat gcagagattg
      121 aacgtctgct gccgcgcgcg aggatgatcg agagccaata aaatcgcatc gactagatcg
      181 tcgaggtgca caaaatttcg ttttacaggt tgcccgttgg ggtcaagcat gcaggggatt
      241 gtttgcgttc gcacatattc atccgcctgc tgcgtgctga ccaaatcgcg ccaacgcggc
      301 cccccaaata catcttcgcc gaacgataat tgatacttga aatcatcctt ttccatgatc
      361 cacggtgcgc gtaagcagca gccgttcaaa ttatattgaa ggtaatactg ttccagcatc
      421 acttcttcca gcaccttgga gagcgcataa caaccgggat atgccgaatg cttttgcgtc
      481 tcggtgacag gcattggatg cggataaaca aaatgtccaa cacccgcatc gccgccaatg
      541 agaacgaatt gctgaaacgt gggactggtg cggcaagtct ccaatagcca gaataatcct
      601 ttcacagcca cattcataat ctcgtcggga gtttctttgc aggttgccag atgcaaaaca
      661 tgagtcacac cgttcagcgc attttcaacc gtctggcgat gttcgatgga accgcgcaca
      721 atttcgacgc gatcattcgc ttctaattcg cgctgatggc ataacgcgcg gacgacaaac
      781 gtgtcaaagt ttcgatctga caagaggtgc ttgataaatg atcggcccac tttgcctgtt
      841 gcgcctgtga ccagaatccg tttttgatca gacatgcaca ctccatttga tctaatatgt
      901 gtcccaactg ttacgacaac gttactcctg ctcttcccgc ttgcctatgt ctaattcgac
      961 caacttaact gtctcggcca acgtcgcgcg ctgtccgact tcgtccaaga ttttttcaat
     1021 ccgatcagct gcttgcgatt gaatggagta cagttcttcg accgatcgtt tcgcagccgc
     1081 tttgtcatca agcgaggcgg cgttaatgcg gacgttcatg cctgccgcat cgatcgcggc
     1141 gcgagccatg aaggccgccg aagccgcgtc gctcagcgca ttgacgttgc catgcgcggc
     1201 gacttcatgc acgagcgata agatttcaag acaggcgcgc gccgcattca gcggcacatc
     1261 ggtggcgtgc tgagtggcgc tttgaatctg tgcagtacgc acagattttt cggcctcggt
     1321 ttccttcggc gatttcatgg cggccatcac ttcttcaaat gcggcagagt cagcatcgat
     1381 cgcttgcgtg agctgcgatc gaagttgatc ggcgcgcgcg gcgatgtctt tcatcgcggc
     1441 atcaacttct gcgtatttct ttttcccgat cgttaaacgc gcgaccatgc cggtcaaccc
     1501 cgccgacaat gctccggcga gcgcggccac tgctccgccg cccggcgtcg cggaaccagc
     1561 cgcagtggct tcaagaaaag cgttgggcac gatcgatttg gctgcgccgc ttgaaatttc
     1621 ggccaacttg ttttcaagaa tctgatggct ttcaaagttg tcgatctgca aataccactg
     1681 agcggcgtcg attaacgctt cctgcggcgt taggccgacg agttcgctct tcacgatcgt
     1741 gtgcccgtaa cgcgccgctt cacgccgcac catctcgacg acacgcgcga tcgaggtttt
     1801 gcggtaatca gtgagattca tcgagacttg cgctttgcca tcgactaaca agccgagtgc
     1861 ttttacaaat ttcaagccgc cgcctgaatt gcgcacaact ttagcaatgt ttttggcgac
     1921 attgacttcc tcggtgttga gatagcagtt gaaggcgacg agaaacggcc gtgcgccgat
     1981 cacagtcgca cccgccgggc cgagatggct gggaccaaaa tcgggtttgc gatttggatc
     2041 agtttcgatc tctgctttga ttccttcgta ttgcccgcgt cggatgtttt ccaagttctc
     2101 tcgatcgggc cgcgtggccg cagcttcata caagtaaacg ggaatattta gttcagcgcc
     2161 tacgcgctgg cctaatcgct tggcaatggc aacacattct tccagcgaaa cgccgctgac
     2221 cggcacgaag ggcacgacat cggttgcgcc caggcgcgga tgctgcccgg tgtgatgatc
     2281 gagatcgatc aactttgcag caacggcaat tccagcgaag gctgcttgct caacggcttt
     2341 cggatcgccg acgaaggtca tcacggtccg gttgtgatcg ggatcagaat gctgatcgag
     2401 aaaggtgaca ccctcgatcg ctgcgatagc cgatcggatt tgggcaacca cctccagccg
     2461 acggccttca ctaaaattgg gaacgcattc gacaattttc ggcatggaaa tttatcctcg
     2521 tgatgaaata tcaggcttat tgtaccgcaa acaaaaaagt ccgactcaag cgagccggac
     2581 tttattggat cgatcttttg tgacattatt ggacgccgag gtaggtgtct tgaacttgct
     2641 ggttgttcct gagttcttca gccgttccgc tcaaggcaat ttggccgact tccatgacgt
     2701 agccgcgatt ggccacttgc aaggccatgg tagcgttttg ttcaacgagc agcacggtcg
     2761 tgccttgctg attgatctct ctaacgatgt cgaagatttg ctcgaccaag atcggcgcca
     2821 gacccatgga aggttcatcg agcagcatga tgcgcggttt cgccatcaac gcgcggccca
     2881 tggccaacat ttgttgctcg ccgccggaca gcgtaccgcc gcgctgcttg aggcgttctt
     2941 tcaagcgcgg gaacaacact aagacacgct ccagatcgct ttgcactccg cttttatcag
     3001 cacgcgagta cgcgcccatt tccaaatttt ccatgacggt catctggggg aaaatcatgc
     3061 ggccttccgg tgactgcgac acaccgcgtt tcacaatttc gtgtgttgga agtttctcca
     3121 gaggttcgcc atccaggatg atagtgcctt cgcggggccg gatcaagcct tgaatggtgc
     3181 gcagggtcgt gctcttaccc gcgccgttgg cgccgatcaa cgtgacgact tcgccatcgt
     3241 tgaccgtaaa ggaaatgcct ttcagcgcgt gaatgctgcc gtaataactg tggacgttgc
     3301 gaagttcgag catgttagtg tcccgccccg ctgccaagat aagcttcaat gacttgctga
     3361 ttcttttgga cttcgaccgg tgtgccttcg gcgatcttct caccgtggtc cagcacgctc
     3421 acccgatcgg agatgcccat cactacgcgc atatgatgtt cgatcaacaa gaccgtcagg
     3481 ctcaagtctt tgcggatgaa ttggatgaat gtagtgaggt ccgccgtctc ggctcgattc
     3541 atgccggcgg tcggctcgtc cagcaagagc agcttgggtt cgctggctaa ggcccgagcg
     3601 atctccaagc gacgttgatc gccgtagggc aagtggcgcg cagtccaatc ggcgcgctgc
     3661 cggccaagtc caacgtagtc caacatgtcc aaagctttac cgcgggccat atcttcctct
     3721 ttggtcatgc gatctgtttt taacaacgta tcgagcaaag tggaatgcaa gcgggtgtgc
     3781 atgccgacca aaacattttc caagaccgtc atcgccggga acaagcggat gttttggaac
     3841 gtgcgggcga tgcccagggc tgtgatgtcg tgtggtttga gcgcgccgat attcgtgtca
     3901 ttaaaggtca aggcgccggt ggtgacagaa taaacgccgg tgatcatatt gaagaaggtc
     3961 gttttacctg cgccattcgg cccgatcagg ccggcgatca tgccctgctc gatgacgaaa
     4021 tcgactttac tgacggcggt cagtccgccg aaggttttgg tgacgccgtg ggcgacgaga
     4081 aagctcatga cgattccacc ttatcaggag ttgctttcag cttggcttca atctcttcaa
     4141 tcttgtcgtc ttcgctggcg tgcagttctt cagcgcgttc gcggctgggg aacaagcctt
     4201 ccggtcgaac ggccatcacc acgaccaaga ccagacccag catcaagaaa cggtatttga
     4261 cgggatcaag attggcttga atgaattgct ggagatcggg attgcccacc gtcgctggca
     4321 gcaagacatg cgtttgaagc ccaatcaaca gattttggaa cgcttttaag atcaagccat
     4381 cattcaagag aatgatcaat gcgccgaaga t
//
LOCUS       DASRTJ010000245         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_271013,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000245 DASRTJ010000000
VERSION     DASRTJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271013"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1136)
                     /locus_tag="VFF70_05105"
     CDS             complement(24..1136)
                     /locus_tag="VFF70_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HZY44112.1"
                     /translation="MRNRSIRFNYVTRLLLIFFWLLAVQWPGGPLITIGPRQTVITNN
                     PKVGIHTRLTDEAAQWKIDRTLQMVRDMGAPWIVEFFPWAYLESSQGSFAWEHADEVI
                     DQAQAHGLTVIARLGLVPAWARPDPRERETTFTYLDHDHYQAFGDFVYAFVQRYKDRV
                     RHIIILNEPNLSYEWGLQPVDPQGYVDLLKIAYQRAKVANPNVIVLAGALAPTLEPIG
                     SLAGLNDVIYLQKMYEAGARNYFDALAAHAYGLQSPLEAAPDPSQVNFRRVELLRQIM
                     IDNGDASKQVFITEAGWNDSPRWNKAVTPAQRIQYTVQSYQWAQQTNWISMVAMWAFR
                     YPQNTRTYQDNWTWVNEGFQPKPIYDEVRKAMTNAQ"
     gene            complement(1399..1614)
                     /locus_tag="VFF70_05110"
     CDS             complement(1399..1614)
                     /locus_tag="VFF70_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44113.1"
                     /translation="MGSNDWKTRTMIVGGILGGLAGVVAAMMFIRSGEASGTERRGRP
                     RVQPRSALQVGIGLMGLLQKISGLAEE"
     gene            complement(1673..1927)
                     /locus_tag="VFF70_05115"
     CDS             complement(1673..1927)
                     /locus_tag="VFF70_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44114.1"
                     /translation="MNQIDRNNSDDSNTPILIIGGLIGVLIGVIGGRLYADADAAARE
                     RDERRAPQGIKFSLPMALPFALTIISLLRQIRDLANNDRY"
     gene            complement(1924..2211)
                     /locus_tag="VFF70_05120"
     CDS             complement(1924..2211)
                     /locus_tag="VFF70_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44115.1"
                     /translation="MISALRDVSLILLIFPVMLCLLIPIGILFGSVWLTGKANKNLRP
                     KVQSAHRSMREVERKVDRVGQRIVRPLVALEMRWVRIQVFLRGLRLERKQP"
     gene            complement(2241..2570)
                     /locus_tag="VFF70_05125"
     CDS             complement(2241..2570)
                     /locus_tag="VFF70_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44116.1"
                     /translation="MAEAPLPVPSKRKDRLKWIIPIALAMMVVCVGCFFILAIGLVSR
                     GELTASAVGTDFRLWSINDRTQTGVALQRSYAVQRADKQCEQFDVTFLIWKPGLSIEN
                     KSYDDCG"
     gene            complement(2616..>2956)
                     /locus_tag="VFF70_05130"
     CDS             complement(2616..>2956)
                     /locus_tag="VFF70_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44117.1"
                     /translation="VDGGAAQSDFDLVDLKVTDLVLKTGASSTVIRLPKAAGMTNVRV
                     ESGAASVRLTVPDGVAARIRSTMGLGSSNIDQRRFPRSGDYYQSPDYDSAANKIDVRA
                     EGGVGSLSVN"
BASE COUNT          704 a          850 c          731 g          671 t
ORIGIN      
        1 gcgtgttaca tgtgtgtcat ttgtcattgt gcattggtca ttgctttgcg cacttcatca
       61 tagatcggct tcggttgaaa accctcgttg acccaagtcc aattgtcctg atacgttcgt
      121 gtattctgcg gataccgaaa cgcccacatg gcaaccatcg agatccaatt agtttgttgc
      181 gcccattgat aactttgcac ggtatattga attcgctgcg cgggcgtaac ggccttattc
      241 caacgcgggc tatcgttcca gcccgcttca gtaatgaaga cttgtttgct cgcatcacca
      301 ttatcaatca tgatctgccg cagcaattcg acgcgccgaa agttgacctg actcggatcg
      361 ggggcggcct ccagcgggga ctgtaaaccg taagcatgtg cggctaatgc atcgaagtaa
      421 tttcgcgcgc ccgcctcgta cattttttgc aaatagatca catcgttcag acccgccaga
      481 ctaccgatcg gttccagcgt cggggcaagc gcacccgcca acactattac atttggatta
      541 gcgaccttcg cgcgctgata agcgatcttc aacagatcga catagccctg cggatcgacc
      601 ggttgcagtc cccattcgta actaagattc ggttcattca aaatgataat atgccgcacg
      661 cgatctttgt accgctgaac aaaagcatac acaaaatcgc cgaaggcttg ataatgatcg
      721 tggtcaaggt acgtgaaagt tgtttcgcgc tcacgcggat cgggccgcgc ccaggcaggc
      781 accaacccca aacgcgcgat gaccgtcaag ccgtgcgcct gagcttgatc gatcacttcg
      841 tcggcatgtt cccaagcgaa tgagccttga ctagattcaa ggtaagccca cggaaaaaac
      901 tcgacaatcc acggcgcacc catatcacgc accatctgca gcgtacgatc aattttccat
      961 tgagccgcct cgtccgtcag gcgcgtatga attccgactt taggattgtt cgtgataacg
     1021 gtttgccgtg gcccgatcgt gatcagcgga ccgcccggcc attgcacagc caacagccag
     1081 aagaagatca acaggaggcg agtgacgtag ttgaatcgaa tcgatcgatt gcgcatcaag
     1141 tattagactc gtcattccga gcacttcggt ttcgctcagt gtaaacaccg cgaggaacac
     1201 atgcacttct atgcgttgcg atgcaagtat ctctacgact gattggcaat gctatcgggc
     1261 gaagtagaga ttcttcgctc cctgcggtcg ctcagaatga cagctcactg acaatcaaaa
     1321 atcccacggc tggaagccga cacagatatt ttatccgtgt ttactttcag ccgtgggaac
     1381 aaaaagcagt gactgaactt attcttcagc taatccggag atcttttgca gcaagcccat
     1441 caagccaata ccgacttgca gggccgatcg aggctgcacg cgcggtcggc ctcggcgttc
     1501 tgtaccggac gcttcaccgg agcgaatgaa catcatcgcc gccactacgc cagcgagacc
     1561 gcccaatatt ccaccgacga tcatcgtgcg agttttccag tcgtttgatc ccattgcctc
     1621 acctcagtcc aagccggata tgagtagttt cgaaaacttc ggaagcctca ttttaatatc
     1681 gatcgttatt cgctaagtct ctgatttgcc gaagcaaact gattatcgtc agggcaaacg
     1741 gtagcgccat cggcagagaa aacttaatgc cctgtggtgc gcggcgttca tcgcgctcgc
     1801 gtgcggctgc gtctgcatcg gcatacaatc ggccgccgat gacaccgatc aacacaccga
     1861 tcagtccgcc gatgatcaag atcggcgtat tcgaatcatc cgaattattt cgatcgattt
     1921 ggttcatggc tgcttccgct cgaggcgcag accgcgcaga aaaacctgga ttcgcaccca
     1981 acgcatttcg agcgcgacaa gcggtctgac gatccgctgc ccaacccgat cgactttgcg
     2041 ctcgacctcg cgcatcgatc gatgggccga ttgcactttg ggccgtaaat ttttattggc
     2101 tttgcccgtc agccacacac tgccgaacaa gatccctatc gggatcaata ggcagagcat
     2161 caccggaaag atcaagagga tcagcgagac gtcgcgcagg gcagatatca tggttgccgt
     2221 ccattatagc gcaagtaaaa ttatccgcaa tcatcgtagg acttgttttc gatcgacaag
     2281 cccggcttcc agatcaagaa cgtgacatcg aactgctcac actgtttatc agcccgctga
     2341 acagcatacg atcgctgcag cgcaactccg gtctgcgttc gatcgttgat cgaccacaag
     2401 cgaaagtccg tgcccaccgc cgaggcggtc aactcgccgc gtgacacgag gccaatcgcc
     2461 agaatgaaga aacagccgac gcatacgacc atcattgcca aggcgatcgg tatgatccat
     2521 ttcaggcgat ctttacgttt tgacggaaca ggtaacgggg cttcagccat ttgcaaattt
     2581 ctgctgcgtg cccttgcgga ttatttcgtg gaacattaat ttaccgacaa cgatccgacg
     2641 ccgccttcag cccgaacatc gatcttgttc gcagctgaat cgtaatcggg tgattggtag
     2701 taatcgccgc tgcgcgggaa gcgccgttga tcgatattgc tggagccaag tcccatcgtc
     2761 gatcgaatgc gcgctgcgac gccatcgggg acggtcaagc gcaccgaggc cgcgccggat
     2821 tcaacgcgca cgttcgtcat gcccgcggcc ttcggcaatc gaatcaccgt gctgctggca
     2881 cccgttttca aaacgaggtc cgtcacttta agatcgacta gatcgaaatc gctctgcgcc
     2941 gcaccaccat cgactc
//
LOCUS       DASRTJ010000246         5743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_316863,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000246 DASRTJ010000000
VERSION     DASRTJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316863"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFF70_05135"
     CDS             <1..342
                     /locus_tag="VFF70_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44118.1"
                     /translation="RSGQPLGYAAYRIAAEGKRKIGFIAEVITNPQGGPVWKTLLSQV
                     LGLLREAQVETAVTLAVPDTEGHAAFRRNGFFGRAAFNVQMVPLDSNLPIERLQDRMR
                     WLINGSDFDVI"
     gene            332..1216
                     /locus_tag="VFF70_05140"
     CDS             332..1216
                     /locus_tag="VFF70_05140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZY44119.1"
                     /translation="MLSSRSIKRTLSGVLENFWSLGFVAAGHFDRRTVDRLISSTQQR
                     ILIVAPHPDDEVIGCAGTLKLQQQFGAEACVVYATDGRRSRAQGLSSAEMANRRKQEA
                     DAALRQLQIAQPHWLGLVEGEWTGDQFTHLLDPILRNFQPHIIYAPSLIDYHPEHKSV
                     AQALARTLKHVRSDFDQPIIRIYQVHVPLTSILVNVVADVSRWGTEIRLAGAAYVTQT
                     DSIHRADRIRRYAAAYYRAGSIAEEFWQMSADRYIDLHLSKNSSNHFRGVRYWAWSDP
                     LAYGFGRNARQSAAKTIR"
     gene            1213..2769
                     /locus_tag="VFF70_05145"
     CDS             1213..2769
                     /locus_tag="VFF70_05145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44120.1"
                     /translation="MSKSIYRISLFTLLLAYWLISLSNITSVPRVYEDEPWQASTGLK
                     IAEQGIFGSDMFSGFYNMQDRYYGYVPLHPILLSIIFRFAGDGLFQSRFEPIALGLLL
                     LALTFSLARRVFTDDRIGLLSIIFLLLVRTSGLTPSQTSGILLIDFARIARYDMLAAV
                     FGLASLHAYQWAYQRSTRRSYMLAGFLAACATLSHLYGVFWLIALFTLTVWNQSRTIS
                     RRSTIGSMLIGFSVPLIPYAIYILGDVADWRGQTLGYADRFELLNVNWYVQNFLHEPQ
                     RYAPGLGSIGFNWLLHPGFWSALIVIPCAVAMLWRRSLSDRTLRALVVPALGLPVLFA
                     LLIHLKLANYLLTIIPILGIVFAWGVVSLWSWLRGRKWIAAQVVVSAVLVASVFEGIA
                     RIVVLEETAMSMTPYTAFIDRVRHFIPNGARVLGLHNYWFGLESFEYRSFALPFLLQQ
                     SNNLSIDQGLEQIEPDVVLIDPRMRETFAIDAQAAEQFQVWLTRHAGQVIGRVDDPTY
                     GLMEVYQVTR"
     gene            2814..4703
                     /locus_tag="VFF70_05150"
     CDS             2814..4703
                     /locus_tag="VFF70_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44121.1"
                     /translation="MIRFPFLAGDFLQQAAPVIGLWEDVEAQHRAQATDYPYDLLRAA
                     DAIIPREAAVLLVTSAQDVRHRGYTTYHRALYDLTPRSIWWAASAPSDGTWEARWWIS
                     TSIDAASISTLANQLHADYILAYDVPALSLGVEIARWPEGYLLQLNETNHAKVGLENN
                     HSILKPSRGSSLGVFWPLQLIIGLLMIFMAGKVILLIIERTFARHFSLATERIALIWT
                     LGSGFVSIGMMWLNALGLSLTGQVSALSILAVVSFSWLHRSQFSRLRFERVQFRIKQK
                     LSGSTVVTSLILFLALQILFVMIMSIGQPLHYWDSWTIWGMKARTIFVEGGLSPAVYL
                     DPSRAVTHLDYPLMLPLIESWLYAWLGTPDDRLVGSASVLFYLSLLAIVYASVRRRGL
                     SLQWSLTITTVLASMAGFAGASGAVSADIPLAALMTVSLSYALEWLDTGSTSALVVAV
                     LSASLLPWLKREGVVLLLALMVAAVLVCRNRRALSMAGWMIVCAVGVNAPWWILIGSQ
                     GVPNTDFVPITAAAFFNNLNRFPTIAYRVMGSGLSLDWNLLNPLLAVSLLALAVRHWR
                     QPLRVHLTARDVLLVAPILYIALISSSYIFSSHVPYQQHVVSSIDRLIAQVMPLPILW
                     LADRV"
     gene            4700..>5743
                     /locus_tag="VFF70_05155"
     CDS             4700..>5743
                     /locus_tag="VFF70_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44122.1"
                     /translation="MIDRSVQSAQTIGFIDRHARLIALSILLIGLLIRLPFLAIDFGK
                     TTDISVYAQWAGRIQAQGLINLYAPGGLVNYPPLFLYWLAAAGSIRPADAALVALIKL
                     PSILADVLTAAIMMALIWKRSRRAAVVVGAVYLFNPAIWYVSAYWGQTDALYTLFLVC
                     ALGLLAHDRSTWAWIIFSLALMTKIQSLAVLPLFVVATLSRRNKIDLVRGGLAAIAVI
                     ALITLPWWLSGKASLMIQSWFLTPNSVSRVDISAYNFWYLALGSQVHAVTASAHPFNW
                     TVSYRLLGFGVYGAFLALATLLIWRSQARSIFLAGTILNLGLFVLFIDIHERYLFPAI
                     ALSLLAAVIDARLR"
BASE COUNT         1083 a         1455 c         1615 g         1590 t
ORIGIN      
        1 cgatcaggcc agccgcttgg ctatgcggct tatcgaattg cagccgaagg caaaagaaag
       61 atcgggttta ttgcagaagt aatcactaat cctcaaggcg gcccggtttg gaaaacattg
      121 ttgtcgcaag tgcttggctt attgcgagag gctcaagttg aaacagccgt cacattggcc
      181 gtgccagaca cagaagggca tgccgctttt cggcgcaatg gatttttcgg acgtgccgcc
      241 ttcaatgtgc aaatggtgcc gctcgattca aatctgccga ttgagcgttt gcaagatcga
      301 atgcgttggc tgatcaacgg aagcgatttc gatgttatct agccgatcga tcaagcgcac
      361 actttccggc gtgctcgaaa atttttggtc tctcggcttt gttgctgccg gacatttcga
      421 ccgccgcaca gtcgatcggc tgatatcatc cacgcagcag cgtatcttga tcgtcgcgcc
      481 gcatcctgat gacgaagtca tcggttgcgc gggaacgctg aaactccagc agcagtttgg
      541 cgctgaagcg tgtgtcgtct atgccaccga cggccgtcga tcgcgagcac aaggtctgag
      601 ttcagcggag atggcaaatc gacgcaaaca agaagccgat gcggctcttc ggcagttgca
      661 gatagcacaa cctcattggc tgggattggt tgaaggtgag tggacgggcg atcaattcac
      721 ccatctgctc gatcccatct tgagaaattt ccagcctcac attatctacg cgccttcact
      781 catcgattat catcctgagc ataaatcggt ggcgcaggct ttagcacgaa cgttgaagca
      841 cgttcgatcg gatttcgatc aaccgattat ccgcatctat caagttcacg tgccgctgac
      901 atcgatcttg gtgaatgtgg tagcggacgt ttcgcgttgg ggcacagaga ttcgattggc
      961 aggtgcggct tacgtgacac agacggacag catccatcga gcagatcgca tccgccgtta
     1021 cgccgcggct tactatcgcg ccggatcgat cgccgaagaa ttttggcaga tgtcagcgga
     1081 tcgctatatc gatctgcact tgtcgaaaaa tagttccaat cattttcgcg gtgtccgtta
     1141 ttgggcgtgg agtgatccgt tggcctatgg gttcggacgc aatgcaaggc agagcgctgc
     1201 taaaacgata agatgagcaa atccatttat cgcatcagtt tattcacact gttgttggcc
     1261 tactggttga ttagcctcag caacattacg agtgtgccgc gcgtctatga agacgagccg
     1321 tggcaagcgt ccacgggctt gaagattgcc gagcagggca ttttcggttc ggacatgttc
     1381 agcggctttt acaacatgca ggatcgttat tatggttatg tgccactgca tccgattttg
     1441 ttatcgatca tttttcgatt tgcgggtgat ggcctgtttc aatctcgatt tgagccgatc
     1501 gcgttaggct tgttgttgct ggctttgact ttcagcctcg cgcgccgagt gttcacagat
     1561 gatcgcattg ggctcttatc aattatcttc ctgctattgg ttcgcaccag cggactgacg
     1621 ccttcacaaa cctccggcat tctcttgatc gattttgcgc gcattgcacg ttatgatatg
     1681 ctggccgctg tcttcggctt ggcatcactg cacgcttatc agtgggcgta tcagcgctca
     1741 acacgtcgct cgtacatgct ggctggtttt ctggcggcct gcgccacgtt gtcacattta
     1801 tatggtgtct tttggctgat tgcgttgttt acactaactg tctggaatca atcgaggacg
     1861 atatcgcgtc gatcgacaat cggctcaatg ctgattggtt tcagcgtgcc gctgattcct
     1921 tatgcgattt atattctcgg cgacgtagcc gattggcgcg gacagacgct tggctatgcc
     1981 gatcgatttg agctgctcaa cgtgaattgg tacgttcaaa attttctgca cgagccgcag
     2041 cgttacgccc ccggtctcgg atcgatcgga tttaattggc tgctccaccc cggattttgg
     2101 agtgcgttga tcgttattcc ttgtgctgtg gcaatgctgt ggcggcgatc actctccgat
     2161 cgaaccttgc gcgctttggt cgtcccggca ctcggcttgc cggtgttgtt tgcgttgttg
     2221 atccatctca agttagccaa ttatctattg acgatcattc cgatcttggg aattgtcttt
     2281 gcgtggggcg tcgtctcact ttggagttgg ctgcgcgggc gaaaatggat cgcggctcaa
     2341 gtcgtcgtca gcgcggtatt ggtcgcgagc gtcttcgagg gtatcgcgcg catcgtcgtt
     2401 ttggaagaaa cggctatgag catgacgcct tacaccgcat tcatcgatcg agttcggcac
     2461 tttattccga acggcgcgcg agtgctgggc ctgcacaatt attggttcgg cctcgaaagt
     2521 tttgagtatc gatcgttcgc gctgccgttt ctattgcagc aatccaacaa cttgtccatc
     2581 gatcaaggct tggagcaaat agagccggat gtcgtgctga tcgatccgcg catgcgagaa
     2641 acgtttgcga tcgatgcaca ggctgcggag cagtttcaag tgtggctcac tcggcacgcg
     2701 ggacaagtca tcgggcgcgt cgatgatccg acgtacggat tgatggaagt ttatcaagtc
     2761 acgcgatgaa gacattgatt aggggcggcc tactaatttg tttggcaatc ggcgtgatcc
     2821 gatttccttt tttggctggc gactttctac aacaggctgc gcccgtgatc ggcctgtggg
     2881 aggatgtaga agcgcagcat cgtgcgcaag ctaccgatta tccttacgat ctgctgcgcg
     2941 cggcagatgc gatcattccg cgcgaggccg cggttttgtt ggtcacttca gctcaagacg
     3001 tgcgtcaccg tggctacact acctatcatc gcgcgttata tgatctaacg cccagatcga
     3061 tctggtgggc agcctctgca ccgtccgatg gcacctggga agcgcgctgg tggatttcca
     3121 catcgatcga tgccgcgtcg atcagcacct tagcgaatca actgcacgcc gattatatct
     3181 tggcatatga tgttccggct ttgtcgctcg gcgttgaaat tgcgcgttgg ccggaaggat
     3241 atttgttgca attgaatgag acaaaccatg cgaaggttgg acttgagaat aatcactcga
     3301 tcttgaaacc ttcgcgaggt tcttcgctgg gcgtgttttg gccgcttcaa ttgatcattg
     3361 ggctgttgat gatcttcatg gctggaaaag tgattctctt gatcatcgag cgtactttcg
     3421 cgcgtcactt ctcactcgcc acagaacgca ttgctctgat atggacatta ggctccggct
     3481 ttgtctcgat cggtatgatg tggctcaacg cgcttggctt gtcgttgacc ggacaagtca
     3541 gcgccttatc gatcttggct gtcgtcagct tcagctggtt acatcgatct caattcagcc
     3601 gcctgcgctt tgagcgagtg caattccgca ttaagcagaa actctctggc tcaacggttg
     3661 tcacgagcct gatacttttt ctcgcccttc agattttatt tgtcatgatc atgtccatcg
     3721 gacagccact gcattactgg gacagctgga cgatctgggg gatgaaagcc cgcacgatct
     3781 ttgtagaagg cggtctctca cccgccgtct accttgatcc atcacgcgcg gtgacacatt
     3841 tggattatcc actcatgctg cctctgattg aatcttggtt gtatgcctgg ctcggcacgc
     3901 ccgacgatcg attggtaggg agtgcgtcgg tgttgtttta tctgtcgttg ttggcaatcg
     3961 tttatgcgtc ggtgcgtcgt cggggcttga gtcttcaatg gtcgttgacg atcaccaccg
     4021 tgttggcttc aatggcaggc tttgctggcg ccagcggcgc ggtgtccgcc gatattccgc
     4081 tggccgcttt gatgacggtt agcctcagct atgcgcttga atggcttgat actggatcga
     4141 ccagcgcttt ggttgtagct gtgctgtccg ccagcctgct gccgtggctg aaacgcgagg
     4201 gggtcgtttt gctcttggca ttgatggtgg cggcggtgct cgtctgccgc aatcgccgcg
     4261 cgttgagcat ggcgggctgg atgatcgtgt gtgccgttgg cgtgaatgcg ccgtggtgga
     4321 ttctgatcgg ttcgcagggc gtaccgaata cagattttgt accgatcact gctgcggcat
     4381 ttttcaacaa cttgaatcgg tttccaacta ttgcctatcg agtgatgggc agtggactga
     4441 gtcttgattg gaacctgctc aatccgttgt tggcggtaag tctgctggcc ttggcggtca
     4501 ggcattggcg gcaaccgctg cgcgtacact taacggccag ggacgtattg ctcgtcgcgc
     4561 cgatcttata catagctctg ataagttcca gttatatttt ttcgagtcat gtcccttatc
     4621 aacagcatgt cgtcagttcg atcgatcgat tgatcgcgca ggtcatgccg ctgccgatct
     4681 tgtggttggc tgatcgagta tgatcgatcg atcggttcaa tctgcacaaa cgatcggctt
     4741 tatcgatcga catgcgcggc tgatcgctct atcgatcttg ctaatcggct tgctgatccg
     4801 tttgccattc ctcgccatcg attttggcaa gacgaccgac atcagcgttt atgcgcagtg
     4861 ggccgggcgt attcaagcgc agggcttgat caacctgtat gcgccgggcg gcttggttaa
     4921 ctatccgccg ctatttctat actggctggc ggcggccgga tcgatccgcc ccgctgacgc
     4981 agcattagtg gcgctgatca agctaccgtc aatcttggct gatgtcttga ctgctgcgat
     5041 catgatggcg ctgatctgga aacgatcgag gcgcgcggcg gtcgtggtgg gtgcggttta
     5101 tctgttcaat ccggctattt ggtatgtatc agcctattgg ggacagacgg acgcgctgta
     5161 tacgttgttt ctcgtgtgtg cgctcggtct gctagcgcac gatcgatcga cttgggcgtg
     5221 gattattttt tcgttggcgc tcatgaccaa aattcagagt ctggcggtgt tgcccctgtt
     5281 cgtcgttgcg acattgagcc gccgaaacaa gatcgatctc gtgcgcggcg gtctggccgc
     5341 gattgcggtg attgcattga tcacgctgcc gtggtggttg agcggcaagg cttcgcttat
     5401 gatccaatcc tggtttctta cacccaattc ggtatcgcgc gtcgatatca gcgcttacaa
     5461 tttttggtat ctggcactgg gcagtcaggt gcatgcagtt actgctagcg cgcacccgtt
     5521 caactggacg gtatcttatc gcctgctggg ctttggagtg tatggcgcat ttcttgcgct
     5581 cgccacacta ttgatctggc gcagccaggc tagatcgatt ttcctggctg gcacgatttt
     5641 gaatctgggg ttgtttgtgt tgttcatcga tatacacgaa cgctacttat ttccggccat
     5701 tgcgcttagt ctgctggccg ccgtcatcga tgcgcgcttg cgt
//
LOCUS       DASRTJ010000247         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_119492,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000247 DASRTJ010000000
VERSION     DASRTJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119492"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2730)
                     /locus_tag="VFF70_05160"
     CDS             complement(70..2730)
                     /locus_tag="VFF70_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZY44123.1"
                     /translation="MFGSVSSAFAQAGGEDEFVARLIAQMSPEAKVGQLFVVSFKGID
                     AALNSDVADLILNYRVGGVVLSIPNGNIINGTDTPTQVATLTAALQNLAKQTNRTGNA
                     PFIPLFIATEQTGDGPPNSQITAGLTPLPSLMAIGATWNADDAEKIGEVAGKELSAIG
                     INLLLGPSLDVRTSPSNTALDPGVNIFGGDPYWVGVMAQAYVRGLRAGSADRLATVLK
                     NFPGQGNLDTEAYTIDRSLDDLKKIDLPPFLRLLQVPTGKTRPMADALLTTNARYRGF
                     SGNIRERTRPISLDNAALQTLIDLPDMKTWREASGLIVSDAVGASMVRDYYATAANAG
                     MTAQQAALESFQAGNDVLILRNLTVLNNSADEATLTRGVINFFRQKYSTDPAFQARVD
                     SAVQRVLRLKYRLYPNYDPAKVVVSINGLRASLGTGGAGVTQVANDALTLLYPSLDRL
                     TLPAPKPDDTFLIAADDHLVQDCKTCPPRSTLTTDGLAQAIEKLYGVPTANITSTRFI
                     DLKAFAVGAPNAIDLTEAFNQSTWIILAMQASDPNVPSSDAAQALIDLRPGLLTNKRV
                     IGFLFGPPQGLTLEQLSRFSAVYALYSKTPPFIDVAVKALSAREPAPGRSPINLAELN
                     YDLTVQTEPDPAQVIQLSLGAEVVEGQPTPAPVNLRIGDNVRIHTNVILDRNGHAVPD
                     GTPVRFTFQFGTETVAGTAQTVSTQNGVARAEFILDRLGLLFIHASSEPALSSITIQI
                     SIGGPGGVGQAATLLPPTPTFTPVPATVKPLAPTPTITPTPEPSFMESVADRSQRANW
                     GELIFALIGVVLISGGSLWRARHEQVELAKAFQAAVWAAISGMIAYVLFALGILASDW
                     LRSIFGLLASLLITVVGGWLALMWLRRNHS"
     gene            complement(2827..3654)
                     /locus_tag="VFF70_05165"
     CDS             complement(2827..3654)
                     /locus_tag="VFF70_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44124.1"
                     /translation="MSPLVAILILALIALFLYWQLVLAEGAYLGKRVVAFLYNITAKK
                     YNHIKHYSAKSDAEYLGIPLSLSLQGTTTPLVLDVATGTSRLPLTLFNQKSFRGQVVA
                     LDNARRMLNEATLYVAAYRDRLTWVWQHSVPLPFEDNSFDAVTCLEALEFMPGTPAAL
                     QECIRVLKPGGILLVSNRISSGRWQMPGKTLRQQNFEAMLKAFGQSDVTTQIWQIDYD
                     LVWSIKPAGPTDTRSADALDALRCPTCQAKFQREEQALICANQHRFPIGADGVIEML"
     gene            complement(3723..4586)
                     /locus_tag="VFF70_05170"
     CDS             complement(3723..4586)
                     /locus_tag="VFF70_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44125.1"
                     /translation="MSIETGQLIRLHIPTRERALWLGPAWAVICGLVASGTFAWRGQD
                     LFFAVLAILLADGIWATVWWTLVETNWSDVFARWSQVSINQPVRSLPFTQPGTAADRA
                     QHWISRSSIWFRSELWPQLGSSVLTALVSIGLGVVLSIIIGWQGLALSLAALALVQIA
                     LLVRRVRRGAVSIVHGWLDIGLAWTLGHAALNDVSLISFAIAILFSFAYSGAIEIIQH
                     EKSARRWLIPQLLVAIVLIVLQHPLAAFALITVLVAQALLSTVLGGHAFTRSAQWWLM
                     LSMLIAALAIR"
     gene            complement(4680..>5182)
                     /locus_tag="VFF70_05175"
     CDS             complement(4680..>5182)
                     /locus_tag="VFF70_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44126.1"
                     /translation="VSILRPAEVYGPRNHAWTVLPVQLAKRGLPSLVGGGHGLTHPAY
                     IDNLIDAYLAAAVCPEAIGEAFNICDGDVEWREFFGRFALMAGRQARSIPVACVWLGT
                     LFAEIGAAITFQPAVYNRSMIGFVTSRSRLSTAKAQRLLNWSPRISFDQGMQRTEAWL
                     RAEKYL"
BASE COUNT         1209 a         1449 c         1451 g         1073 t
ORIGIN      
        1 atcgatcaca ccatctgcgc tctcgtgtgg atgctaacca acatgaatca ctcgggtcat
       61 gcgtcgcccc tacgaatgat ttcttctcag ccacatcagc gctaaccaac cacctaccac
      121 tgtaatcaac aaagaagcca acaacccaaa tatcgatcgg agccaatcgc tggcgaggat
      181 gcccaacgcg aacaacacat aagcgatcat gccgctgatt gcagcccaga ctgcggcttg
      241 aaaggccttc gctaattcca cttgctcatg gcgcgcacgc cacaaactgc cgccgctgat
      301 caacaccacg ccgatcaacg cgaagatcaa ctcgccccaa tttgcccgct gcgatcgatc
      361 ggcgactgat tccatgaaac tcggttcggg cgtgggcgta atcgttggtg taggcgcgag
      421 cggttttacg gttgccggga caggtgtgaa agtcggcgtg ggcggcaaaa gtgtggctgc
      481 ttgaccgacc ccgcccggac cgccgatcga aatctgaatc gtgatcgaac tcagcgccgg
      541 ttcactggag gcgtggatga ataacaggcc gaggcgatcg agaataaact ccgcacgcgc
      601 gacgccattt tgtgttgaga ctgtttgagc cgtacccgcc accgtctcag tcccaaattg
      661 aaatgtgaaa cgcacaggtg tgccgtctgg caccgcatgg ccgttgcgat caagaatcac
      721 gttggtgtgg atgcgcacat tatcgcctat acgcagattc accggcgcgg gtgtgggctg
      781 tccttcgacc acttcagcgc cgagcgacaa ttgaatgact tgcgcgggat caggttcggt
      841 ctggacggta agatcgtaat ttaactcagc cagattgatg ggcgatcggc cgggcgctgg
      901 ctcacgggcc gataaggctt taacagccac atcgataaac ggcggcgtct tgctatacaa
      961 cgcatatacg gcgctgaagc gcgacagttg ctcaagcgtt aaaccctgcg gcggcccgaa
     1021 caaaaaccca atcacgcgtt tattcgtcaa taggcctggc cgcagatcga tcaatgcttg
     1081 cgccgcatcg gacgacggca cattcggatc gctggcctgc atggccaata tgatccacgt
     1141 cgattggttg aaggcctcag tcagatcgat cgcattcggt gcgccgaccg caaaagcttt
     1201 gagatcgatg aagcgcgtgc tggtaatgtt tgccgtcggc acgccgtaca gtttctcgat
     1261 cgcttgggcc agcccatcgg tggtgagcgt cgatcgcggc ggacacgttt tgcaatcctg
     1321 cacgagatga tcatcggcgg cgatcaggaa cgtgtcgtcc ggtttcggcg cgggcagcgt
     1381 caagcgatcg agcgacggat agagtaaagt caaagcatca ttcgctactt gagtaacccc
     1441 agcgccgcct gtgcccaggc tcgcgcgcaa gccattgatc gatacgacga ctttggcggg
     1501 atcgtaattc ggatacaacc gatatttcaa gcgcagcacg cgttgcacgg cgctgtcgac
     1561 gcgcgcttga aaagccggat cggttgaata tttttgccgg aagaaattga tcacgccgcg
     1621 cgtcagggtc gcttcatcgg cggaattatt cagcacagtc agattgcgca gaatcaacac
     1681 atcgttgccg gcttgaaaag attccagtgc cgcttgctgc gccgtcatgc ccgcgttcgc
     1741 cgccgtcgcg tagtagtctc gcaccatcga cgcgcccacc gcatcgctga cgatcaggcc
     1801 actggcctcg cgccacgttt tcatgtccgg cagatcgatc aaagtttgca gcgcggcgtt
     1861 gtctaaactg atcggccggg tgcgttcgcg gatattgcca ctgaagccgc gatagcgcgc
     1921 attagtcgtc agcagcgcat cggccatcgg gcgtgtttta ccggtcggta cttgcaacaa
     1981 ccgtaagaag gggggtagat cgatcttctt caaatcatcc aatgatcgat cgatcgtata
     2041 ggcttcggta tccagattgc cctggcccgg aaaatttttc agaacggtcg ccaaccgatc
     2101 ggctgatcca gcccgcaggc cccgcacgta cgcttgggcc atcacgccca cccaatacgg
     2161 atcgccgccg aaaatattga caccgggatc gagcgccgtg ttggagggcg acgtgcgcac
     2221 atcgagcgac gggccaagca gtagattgat gccgatagcc gatagttctt taccggcaac
     2281 ttcaccaatt ttttcagcgt cgtctgcatt ccacgttgcg ccgatcgcca tcaggctggg
     2341 cagcggcgtg aggccggccg ttatttgact attcggcgga ccatcaccag tttgttcggt
     2401 ggcgatgaaa agtggaatga agggcgcgtt accggtgcgg ttggtttgct tcgccagatt
     2461 ttgcagcgcg gccgtcaacg tagcaacttg ggtgggtgta tccgtgccat tgatgatgtt
     2521 gccattgggg atcgataaga ctacaccgcc gacgcgatag ttcaggatga gatcggccac
     2581 atcgctgttc aaagcggcat cgatgccctt gaagctgacg acgaataact gcccgacttt
     2641 cgcttcaggc gacatctgcg cgatgagccg cgcgacgaat tcatcctcgc cgccggcttg
     2701 agcgaacgcg ctgcttaccg atccaaacaa tagcgccgca atgactatgc aattgaggaa
     2761 tcgaaaaagt cgaggcatca tcgccgttta gtataacttg cccgattgaa tgcgtcaatt
     2821 cagcactcac agcatttcga tcacgccgtc tgcgccgatg gggaagcgat gctgattggc
     2881 gcagatcagt gcttgctctt cgcgctgaaa cttggcctgg catgtgggac agcgcagcgc
     2941 gtccagtgca tcagccgatc gggtgtcagt tggcccggcg ggcttgatcg accagacgag
     3001 atcgtaatcg atctgccaga tttgtgtggt cacatcactt tggccgaagg ctttcagcat
     3061 cgcttcaaaa ttctgttgcc tgagtgtttt gccgggcatt tgccagcgcc cgctggaaat
     3121 tcgattggag actagcaata tgccaccggg tttcaacacg cggatacatt cctgcaatgc
     3181 ggctggtgtg ccgggcatga actccaaggc ttctagacac gttactgcgt cgaatgagtt
     3241 gtcttcgaag ggcagcggga cggagtgctg ccagacccac gtcaatcgat cgcggtaggc
     3301 ggccacatag agggttgctt cgttcagcat ccggcgcgca ttatccagtg ccaccacttg
     3361 accgcgaaaa cttttttggt tgaagagcgt cagcggtaaa cgtgaggtgc ccgttgcgac
     3421 atccagcacc aatggcgtgg ttgtgccttg cagcgataga ctcagcggta tgccgagata
     3481 ttccgcgtcg gacttcgccg agtagtgttt gatgtgattg tattttttgg ccgtgatgtt
     3541 atagaggaat gcaaccacgc gcttgcctaa ataggcgcct tcggccagaa caagttgcca
     3601 atacaagaag agagcgatga gggcgaggat cagaatggca acgagcggtg acatgatcta
     3661 aggaatgaag gatgaagtta atcagggatg gtgaattacg cagtctgcat gacgcagccc
     3721 ttctatcgaa ttgccagcgc ggcaatcagc atgctcagca tcaaccacca ctgcgccgat
     3781 cgagtgaagg cgtgcccacc caacactgtg gagagcagcg cttgcgcgac taaaacggtg
     3841 atcaatgcaa acgcggctaa tggatgttgt agaacgatca agacgatcgc gactaacagc
     3901 tgcggaatca accagcggcg tgctgatttt tcatgctgaa tgatctcgat cgcgccgctg
     3961 taagcaaatg aaaagaggat cgctatggca aacgagatca ggctaacatc attcagcgca
     4021 gcgtggccga gtgtccaggc gaggccaatg tccaaccagc cgtgtacgat tgagaccgca
     4081 ccgcgccgca cacgtcgaac gagcagagca atctggacca acgcgagagc cgctaaactc
     4141 aacgcgagcc cttgccagcc gatgatgatg ctgagcacaa cgcctaagcc gatcgaaaca
     4201 agcgctgtca aaactgacga gcctaattgc ggccacagtt ccgatcgaaa ccagatgctt
     4261 gatcggctaa tccaatgctg cgctcgatcc gcagctgtgc ccggctgcgt aaacggcaat
     4321 gatcgaactg gctgattgat cgagacttga gaccagcgcg cgaaaacatc cgaccaattt
     4381 gtttcgacca atgtccacca gaccgttgcc cagattccat cggccaatag gattgcgagc
     4441 acggcgaaaa acagatcctg tccgcgccag gcgaaggtgc cgctcgcgac gaggccgcag
     4501 atcactgccc aggccgggcc gagccacagg gcgcgttcgc gcgtgggaat gtgtaagcga
     4561 atgagttgac cggtttcaat gctcaatgat caatgctcaa ttctcaatgc atcatactta
     4621 ttgcaggtca atctctataa acaagaacaa ctacgcagtg tgcattttga attatacaat
     4681 cacaaatatt tttctgctcg cagccacgct tcagtgcgct gcataccttg atcaaatgaa
     4741 atgcgcggtg accaattcaa caaacgctgc gccttggccg tgctcaatcg actgcggctt
     4801 gtaacaaaac caatcatgga tcgattatat acggccggtt gaaaagtgat cgctgcaccg
     4861 atttcggcga acagcgtgcc gagccacaca caggcgaccg ggatcgatcg ggcttgccga
     4921 ccggccatca gcgcgaagcg gccaaaaaat tcgcgccact ccacatcgcc atcacaaatg
     4981 ttgaaggctt cgccgatcgc ttcgggacaa actgcggcgg ccaaataggc gtcgatcaag
     5041 ttatcaatgt aagccggatg ggttaggccg tgaccaccgc cgaccaacga aggcagcccg
     5101 cgtttggcca gttgcaccgg cagaactgtc caggcgtgat tgcgcgggcc atacacttcg
     5161 gccggccgaa ggatgctaac cc
//
LOCUS       DASRTJ010000248         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_89416,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000248 DASRTJ010000000
VERSION     DASRTJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89416"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFF70_05180"
     CDS             <1..489
                     /locus_tag="VFF70_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZY44127.1"
                     /translation="TKASRKENKIGLSMLEPAKLDWKDIKPGQTYTGKVTKLDRMRAF
                     IEIGAEREATLHVSEMGAGFIDQAADVLKVGEEVEARVIEVDPKKRQIKLTTKIDIDE
                     AMAELAKDEDQTPAVTPMQAAFQSAQQEHASTARTKSHVATSDEHEDIFRRTLERHKQ
                     QN"
     gene            714..1211
                     /locus_tag="VFF70_05185"
     CDS             714..1211
                     /locus_tag="VFF70_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY44128.1"
                     /translation="MKIGEIHTLFDYNYWANQRILTTCAKVTHDQYLASGTFPYISLR
                     GTLIHILDAEQIWRVRCQQAAEASEITELEFPTLSALVALWRKEESAMRTYLASLIDS
                     DLATFVGYKAGNGEPRSRVLWHILIHILIHGSQHRSEAAAILTSFKQSPGDIDFTMYL
                     NTKSL"
     gene            1279..>2141
                     /locus_tag="VFF70_05190"
     CDS             1279..>2141
                     /locus_tag="VFF70_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44129.1"
                     /translation="MDQIISWLMDGDPAIRWQTMRDLLDAPARVWKAERQRTLETGWG
                     ANFLARQAADGRWGGDRSTHSRWGNGIYTPKWTSTTYTLLALIGLGIPNDSKSAQRGA
                     RLVLDTLLGETLEAGFNERLAYCDRCVVGMLLQIGVYFKIDDDRIEAIVDNLLNEIMP
                     DGGWNCRRGGYSKPHHSSFNTTINVLEGLHEYMESRRGTRRKKAIVAEHSALELMLQH
                     RLFKSDKTGNIINSKFKLLSYPPRWHYDVLRGLVYFARADAPRDCRLQDAIDLLHSQR
                     RTDGLWPVQYK"
BASE COUNT          557 a          577 c          553 g          454 t
ORIGIN      
        1 accaaagcct cacgcaagga aaataagatc ggcttgagca tgctcgaacc ggccaaactc
       61 gattggaaag atatcaagcc cggccagacc tacaccggca aggtgactaa actcgatcgg
      121 atgcgcgcct tcatcgagat cggcgcggag cgtgaagcaa cgctgcacgt cagcgaaatg
      181 ggcgcaggct tcatcgatca agccgctgat gtattgaaag tgggcgagga agtggaagcc
      241 cgcgtaatcg aagttgatcc caagaagcgg cagatcaaac tgacgaccaa gatcgacatc
      301 gacgaggcga tggctgaact cgccaaagac gaagatcaga ctcctgcggt cacaccgatg
      361 caggctgctt tccaatctgc gcagcaggaa cacgcatcca ccgctcgcac aaagtcccac
      421 gtcgccacca gcgacgagca cgaagatatt ttccgccgca cgctcgaacg gcacaagcag
      481 caaaattaga ttcgtttgca gtcacacccg cgatggcggg tgtttatcat tttcgttccg
      541 gattcccgct tttggtacac cctgaggggt tttaccaccg cagggcgcgg gaacgacgcg
      601 tattcagcta tgtcgcggcg gctcgatgaa tcgggtcgcc gtgtttcgtt tgatcaaaat
      661 ttcgaagatg tgctaaacta gcgtaaagtg aagatcgata agaggggcac gacatgaaga
      721 tcggagaaat acacacgctg ttcgattaca actattgggc caatcaacga attctaacaa
      781 cctgtgcaaa ggtaactcac gatcaatatc ttgcatccgg cacatttcca tacatcagcc
      841 tacgtggaac gctgattcac attctcgacg ccgagcagat ttggcgcgtg cgctgccaac
      901 aagcagccga ggcatcggaa ataactgaat tggaatttcc tacactcagt gccttagtcg
      961 cgctgtggcg taaagaagaa tccgccatgc gcacctatct cgccagtctg atcgatagcg
     1021 atcttgcaac tttcgtcggt tataaggcag gcaatggtga acctcgatcg agagtcttgt
     1081 ggcacatctt gatccacatc ctgattcacg gcagccagca ccgcagtgaa gccgccgcaa
     1141 tcttgacttc attcaaacaa tcacctggtg acattgactt cacgatgtac ttgaacacca
     1201 agagtcttta gcaagtaccg catcctgcac ttacccaact ctccactaaa acagattgca
     1261 agtgcatgaa gatcgacaat ggatcagatt attagctggt tgatggatgg cgatccagcc
     1321 attcgatggc agaccatgcg cgatctgctg gacgcacccg cgcgagtgtg gaaagccgaa
     1381 cggcaacgta cactcgaaac aggctggggc gcgaattttc tggcccgaca agctgccgat
     1441 ggccgttggg gtggcgatcg cagcacgcac agccgttggg gcaatggcat ctacacgccc
     1501 aagtggacct cgacgactta cacgctgtta gctttgattg gtctcggtat cccgaatgac
     1561 tccaagtcgg cacagcgcgg cgcgcggtta gtattggaca cactgttagg cgagactctt
     1621 gaggcaggct ttaatgagag acttgcttac tgcgatcgat gcgtcgtcgg catgctgtta
     1681 cagatcggag tatatttcaa gatcgatgat gatcgcatcg aagcgatcgt agacaatctc
     1741 ttaaacgaaa tcatgccgga tggcggctgg aactgtcgac gtggcggcta ctccaaaccc
     1801 catcatagtt cattcaatac aacgattaac gtgctcgaag gcttgcacga gtatatggaa
     1861 tctcgccgag ggacacgccg caaaaaagca atcgtcgccg agcacagcgc attagaactc
     1921 atgctgcaac atcgattatt taagtcggac aagactggaa acatcatcaa ctcaaaattc
     1981 aaattgttat cctatccgcc ccgctggcat tacgacgtgt tgcgtggcct ggtctacttc
     2041 gcccgcgccg acgcgccccg cgactgccgt ttgcaagatg cgattgatct gcttcattcg
     2101 caacgtcgca cagatggctt gtggcctgtg cagtataagc a
//
LOCUS       DASRTJ010000249         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_346654,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000249 DASRTJ010000000
VERSION     DASRTJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346654"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="murI"
                     /locus_tag="VFF70_05195"
     CDS             <1..833
                     /gene="murI"
                     /locus_tag="VFF70_05195"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZY44130.1"
                     /translation="NDPRPIGIFDSGVGGLSVWREITEQLPHEDTIYFGDQIHIPYGS
                     RSMEQIRSFSDAMTRFLLDRGCKIIVVACNTASAAALTHLRSTFPEISFVGMEPAVKP
                     AAETTRTGVVGVLATPATFQGALFASVVERFAGDVKLVKETCPGLVQQVEAGELNTPK
                     TRKMLTGFLKPILDTNADTIVLACTHYPFVIDAIRSIAPGINVIDPAPAIARQTDRVL
                     KERGLGADRDRAGQYQFFSSETIEKFRAILKVLVDVEDNAYQAIWHVDAQPIRLTAML
                     "
     gene            898..2862
                     /locus_tag="VFF70_05200"
     CDS             898..2862
                     /locus_tag="VFF70_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44131.1"
                     /translation="MRIYRLDAQSIWYDEGLSIYLAQLPPDQTIALSATTDHPPLHAL
                     LLGGWIRLAGDSDFSVRFLSVFFGVLAAALTYQLGRKFNERAGLIAACLISIAPMAVY
                     YSQETRGYMLLTALILIAAIAFMQLLTGHEARWTWLIYSASIVGALYTHYFAAFAWLA
                     FNVAYFVNFVYQELFNRKGTKAQRITDINPGVLASSRFALWFTVQLIIAVCFLPWLPN
                     AITQAGSNATYFPGRVTWDTVVGDTWHAFTVGEWGGGSIASWVWVVLIAIGVLSSIWQ
                     FIRDRRTEAVEGNNWRLVLAFMLWLFIPIVLMSALAWFKPKFAPRYLLPSLPAFVVLG
                     SIGFANFRPTGTNRFKLSTTPGGPLTRSVRYGQVGVPILCILALLFFDAQALVRQYSD
                     PSIARPDIRSVVAYIQAHEQPADAIMLISGYQAPAFDHYYRGSNDMIPLPPDLLPAPQ
                     SPIDARSIATLADMAATYPRIWLVLWQNAISDPMDIIKDQLVERGRQLEVSQNFNGLG
                     LQLFDVRAAKFASQPEITVAYAFVQPVKLVGYYMNTTRVLVGTSFSIGLYFSTDGSIQ
                     GNYQVFTHLVDNEGSIVAQLDHIAGADSYPTSLWQTGSLILNRFEIAVPNNSPPGKYA
                     LQIGLYDSTGRLKLIDGSDHVDLLTIDVVK"
     gene            3119..>3962
                     /locus_tag="VFF70_05205"
     CDS             3119..>3962
                     /locus_tag="VFF70_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY44132.1"
                     /translation="MATLTLPQTTVRPGPIAESWIKRDHKNLSPSYNRAYAFVMERGR
                     GSEVWDVDGNRYIDMNAGIAVTSIGHSHPAVVQAIKDQAEKFLHMSGTDFYFPAMVRL
                     GEKINSLRPMKEESYVFFNNSGTEAVETAIKLARHATGRSQFIGFYGGFHGRTMGSLS
                     FTASKSIYRKGFSPTMPGVTHIPYCDPYRPILATQPGQDYGDAVIDYLENVVFAQAVP
                     PSDVAGILIEPIQGEGGYVVPTDRFLPRLREVCDKYGILLIADEVQSGVGRTGKWWAI
                     EHWNV"
BASE COUNT          835 a         1030 c         1076 g         1021 t
ORIGIN      
        1 tgaacgatcc acgtccaatt ggcatcttcg attccggagt cggcggcctc agcgtctggc
       61 gcgagatcac cgaacaactg cctcacgaag acacgatcta cttcggcgat caaatccaca
      121 tcccttacgg ttcacgctcg atggagcaaa tccgatcgtt ctcggatgcg atgacgcgct
      181 tcttgctcga tcgtggctgc aagattatcg tcgtggcctg caataccgct tcggcggcgg
      241 cgctcactca tttacgatca acatttcccg aaatatcctt tgttggcatg gagccggccg
      301 tcaagcccgc tgcggaaaca actcgcacgg gcgtcgtcgg agttcttgcg acccccgcca
      361 cttttcaagg cgcgttgttc gccagtgtcg tcgagcgatt tgccggtgac gtgaaattag
      421 tgaaagaaac ttgtccgggg ttagttcaac aagttgaagc gggcgaactc aacacaccca
      481 agacgcgcaa gatgctaacc ggttttctca agccgatcct cgataccaac gctgacacaa
      541 tcgtgttggc ctgcacccat tatccgttcg tgatcgatgc gattcgatcg attgcgccgg
      601 gcatcaatgt gatcgatccg gcgcccgcga tagcgcggca aaccgatcgg gttttgaagg
      661 aacgcggcct tggtgcggat cgagatcgcg cggggcaata tcaattcttc agcagcgaga
      721 cgatcgaaaa gtttcgcgca atcctaaaag tgttggtcga tgttgaggat aatgcttatc
      781 aagcaatctg gcatgtagac gcgcaaccga ttcggttaac ggccatgcta taatcgccgt
      841 gtgtccacgc gatctcgaac gatttacctc tgctgcattc tgctcctcgc ttttgctgtg
      901 cgcatttatc gattggacgc gcagagcatt tggtacgatg aaggcctgag catctatctg
      961 gcgcagctgc cgcccgatca aacgatcgcc ctcagcgcca ccaccgatca tccgccgtta
     1021 catgccctgc tgctcggcgg ttggattcgt ctcgcgggcg atagcgactt ctccgttcga
     1081 tttctctccg tcttctttgg tgttttagcg gctgcattga cttatcaact tggccgcaaa
     1141 tttaatgaac gggcaggttt gatcgctgcc tgtttgatct caatcgcccc catggctgtt
     1201 tattattcgc aagagacgcg cggttatatg ctgctgactg cgttgatctt gatcgcggcg
     1261 atcgctttca tgcagttgct cacaggacac gaagcccgtt ggacttggtt gatctatagt
     1321 gcgtcgatcg ttggcgcttt atatacgcac tacttcgccg cgttcgcctg gctggcattc
     1381 aatgttgctt acttcgttaa ttttgtttat caagaattat tcaaccgcaa aggcacaaag
     1441 gcgcaaagaa taactgatat taaccctggc gtcttggcat cttcgcggtt tgctttatgg
     1501 tttactgttc aactgatcat cgccgtgtgc ttcttgccgt ggctgccgaa tgccatcacg
     1561 caagctggat cgaacgcgac ttattttccg gggcgagtga cgtgggatac ggtcgtcggt
     1621 gatacgtggc atgcgttcac agtcggcgag tggggcggcg gctcgatcgc gagttgggtg
     1681 tgggtggtct tgatcgccat cggcgtgctt tcaagtattt ggcagtttat tcgcgatcga
     1741 aggacggagg ccgttgaggg aaacaattgg aggctggttc ttgcgttcat gttgtggctg
     1801 ttcattccaa tcgtgttgat gtctgcactg gcttggttca agcctaagtt cgcgccgaga
     1861 tatttgctgc cgtcattacc agcgtttgtc gtattagggt caattggatt tgctaacttt
     1921 cggcctaccg ggactaatcg gtttaaattg tcaaccacgc ctggcggccc actgacgcgc
     1981 agcgtcagat atgggcaagt cggagttccg atcctctgca tcctcgcgct tctattcttc
     2041 gacgctcaag ctctcgttcg gcagtattct gatccgtcca tcgcccgacc tgacatccga
     2101 tcggttgtgg cgtatattca agcgcacgaa cagccggcgg acgcgatcat gctgatcagc
     2161 ggatatcaag cgcccgcgtt cgatcattat tatcgcggat cgaatgacat gatcccgcta
     2221 ccgcccgatc tgctgcctgc gcctcaaagt ccgatcgatg cacgctcgat tgcgacgttg
     2281 gcagacatgg cggcaacata tccgcgtatt tggttggtgt tgtggcaaaa cgcgattagc
     2341 gatccgatgg atattattaa ggatcagttg gtggagcgtg gacggcaatt ggaagtgagc
     2401 cagaatttca acggcttggg cttgcagttg ttcgatgtac gcgcggcgaa gtttgccagt
     2461 cagccggaga tcacggtggc ttatgccttc gtgcaacccg ttaaactcgt tggttactat
     2521 atgaacacga cgcgcgttct ggttggcaca tcattctcga tcgggctata ctttagtaca
     2581 gatggatcga tccaggggaa ctatcaagtg ttcacgcatt tggtggataa tgagggatcg
     2641 atcgtggcgc agctggatca catcgccggc gcggattcat atccaacttc cttgtggcaa
     2701 accggcagtt tgattctcaa tcgatttgag attgctgtgc cgaacaattc accgcccggc
     2761 aagtatgcgt tacaaatcgg tttatatgat tcgactggac gtttgaagtt gatcgatggc
     2821 agtgatcatg ttgacttgct gaccatcgat gtagtgaagt agaaattaaa tcgggcaaac
     2881 tatgttgttc atctctcgtt cctctaatcg aagatcctct caaagcgtcg ccgacccgat
     2941 cgttgcccag attaagtggt cgacggccga cgtttgaaga ttacctcctt gctggtctag
     3001 ccgcaggtgt gttgcggcgt catcgcgcga ataatcaatc gggctaaaca cctcgatcgc
     3061 attatcgctt tcttggctat aaccagagcg ctagcgtttt attcatcgga ggcttggcat
     3121 ggcaacactg actctacccc agacaaccgt tcgacccgga cctatcgctg aatcttggat
     3181 caagcgggat cacaaaaacc tttcgccatc gtacaatcgt gcctatgcat tcgtgatgga
     3241 acgcggccgt ggcagcgaag tttgggatgt cgatggcaac cgttatatcg atatgaatgc
     3301 gggcatcgcg gtgacctcga tcggccattc gcatcccgcc gtggtgcagg cgatcaaaga
     3361 tcaagccgaa aaatttctgc acatgtccgg caccgatttt tattttcctg ccatggtgcg
     3421 cctgggcgaa aagatcaata gtctgcgccc aatgaaagaa gagtcgtacg ttttcttcaa
     3481 caactccggc acagaagcgg tggaaacggc gatcaagttg gcgcgacatg caacgggtcg
     3541 cagtcaattc atcgggtttt atggcggctt tcatggccgc accatgggct cgctgtcttt
     3601 cacggccagc aaatcgatct atcgcaaagg tttttcgccg acaatgccgg gcgtgacgca
     3661 cattccatat tgcgatccct atcgacctat cctggcgacg cagccgggac aagattacgg
     3721 cgatgctgtg atcgattacc tggagaatgt ggtttttgct caagccgtgc cgccgagtga
     3781 tgtggccgga attttgatcg aaccgattca aggtgagggc ggctatgtgg tacccaccga
     3841 tcgattcttg ccgcgactgc gcgaagtgtg cgacaaatat ggcatcctct tgatcgccga
     3901 tgaagttcaa tcgggtgtgg gccgcaccgg caaatggtgg gcgatcgaac actggaacgt
     3961 cg
//
LOCUS       DASRTJ010000250         6912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_119616,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000250 DASRTJ010000000
VERSION     DASRTJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119616"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..6898)
                     /locus_tag="VFF70_05210"
     CDS             complement(107..6898)
                     /locus_tag="VFF70_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44133.1"
                     /translation="MATKLQEAQSNSASVALERQQQFEVVQRRVKEMSTLLQTGRAIT
                     SLDLESVLNNLARESAGAVGADRCSIFVIDPRRQVLTLRGWWDFEGVPQPMLLYSLGE
                     GIIGWVAQENRSLFLANAPADPRFIIKWAHDHDIAAVMNVPLKIDEVVVGVLQVATRP
                     GTPSFSRDDQRLLNNFADQAAAAIKNAQLYEVERRRAQEMSVIAEINRTISSSLALDI
                     TLDAILGSVKSLIPYDLAEINLWDATEGVMRTHGRGADPKYAAYSQIRGGVYHIDQGL
                     TGWLARNRLPLLIDDVAQSDVLPMIDREQFPIRSHCGVPLISGDTLIGTLELASYTPS
                     AFTAANLETTRTIAGQAAIAIQNALLFEEAHHRADESASLFRISAVAASGSSPAKLLQ
                     NLMAEICTLLKAQLGLVMLYNADTKQLEPLLSTAYGDLADTVGDFQIDTTRPSFHRSA
                     FATRQVRRFDNALIDRRIAPTYLPLLQHFQARGLLIGPLVTHDQAIGEVYIVKRDNVP
                     FTHDDERRLSTVMALLANAIENARLYEESQRRIEQLSYLSEIGRAMSAALNEDQVLDI
                     LYQQTNRVMDARTLYVAYYDEPQDEVTFARIYEEGALTPLGQDNKRRGENTLTFFVCH
                     QRKALLLRGDLNAEMARLGIQMRLIASGQPAVAWMGVPMIIGDRIAGIISVQHFHDRF
                     AYDQNDLNLLQSIANSAGGALENARLYQLTDVRLGQRVEELTALSAITQELNSTLDQD
                     HIFSLVLDEAARVTGAQYGIVSLVGVDSQSLEVRASLGFERDEALRIQSIRIPLGTGL
                     SGRAAAEGKPIIVNDVSGDPNYMKISPDTRSEIVVPIRYAQSVVGVLNLESTQLHAFT
                     PDHLEFLKALASQAAIAIGNAQRLEEQLVQSDLLRRRAEQLTNLFQIGQAFRTDQPLE
                     KIFDDVVHAIQETVGFNIVVLSVLTGEPPRLKREAAAGIPMATFEKMRQVEQPWKNIE
                     AIMQDEFLISQSYYVPLEKREAADTLDVYMAPGGDLSLVRVPGMWHPEDLLITPLRGS
                     GDRILGLLSVDEPASGRLPDRATIEALELFANQAAIAVENGQLYADLQHRLDNLTLFN
                     EVGRSISAKLDLESLLTTVLDASVELMQASRATVFMRDPLDGKYAPRKAIGFDLTEAR
                     SLRFASGEGLVGSVVAEQRGLIVPDVDQDDRFVEVPVAADVRSMVLVPLIVAAQVMGV
                     LVVDKTTPNGFSNTDLVVLSTLADQAAVAIENARLYEETQRRLREQSLLYEAGQAISS
                     TLNYNQVLETVSSQLLRATSAHMVIIQEWDRVNDKLTTVHTRYLTPEGLKQSELATDT
                     FAPLDYLKVAQFLQDRRSLSLRLNDPELDPSLKDRMRQAGLLWLLEVPIVARGEVLGL
                     VRVGDGRFDRILTDSEIQLMETLVNQGAVAMSNARLYDQVVKFTQELEGRVEERTREL
                     GRANAQLTTERDRVEMLFRITSELSASLDLDRVLNRALELMVAAVAAPYGSILLVDPQ
                     KDTLTLRAVLGDKDPLPLGGKPTAFRKGEGLAGWSVVNRQPVIVPDIQQDRRWAERRE
                     SDREYRSALAAPLIIGEDVLGSVLLLHPQPDYFTESHSRLVAAAATQVATAINNAELY
                     RYIREQAELLGGMLRAQQVEGSKNQAILESVTDGVLVTDARGVVILFNAAAERILDVK
                     RDKVMARSIADMLGLYSAAGPQFVAEVRAWLGSIEARRKSPVLSQLMEFLPESRFVNV
                     TLAPVALGDEYLGSVSVFRDITREVEADRAKTEFVSTVSHELRTPMTSIKGYADLLLF
                     GAAGRVNENQHRFLSVIKSNADRLSVLLNDLLDISRIESGRVKLDFKVLSVESVVEQS
                     LTALRGKIEEKGVHLITDVPERDLPDVTGDRDRVIQILTNLVSNAYQYTPAGGSITVR
                     ARPVDDFVQIDVADTGIGISPESHMKVFDRFFRADDPVVQEFPGTGLGLAIVKSLVEM
                     HEGRIWLESEPDVGTIFSFTLKIAVVESVEELIPVEPVEFRPLARQIDRVARSNGGGR
                     RILVVEDDHDIAELIHQHLTSVGYDVRIASRARDALEQAQIDQPDLITLDIYLPDRDG
                     FEVLQELKTNPATADIPIVIVSVMPDRHEGFRLGAVDYLTKPIDPSRLVASINRILFG
                     KGKVLVVDDDRDTRDFLQTALEARGFSVVLTSSGKRALVLARQEIPDLILLDIKLPGM
                     DGYEVLQKLKNSEDTADIPVMVITGSLTDEELKHTKVMSLGAARFKTKPFAVDELLAE
                     ITDLVKGDLAESMPA"
BASE COUNT         1666 a         2065 c         1644 g         1537 t
ORIGIN      
        1 atcacgttca tcttcagcaa ttaaaatttt ggtcattttc acttatcctc aacttgaccg
       61 ctgaatccag cggcagggtt ctatccagcc agattaccga acgggatcac gctggcatcg
      121 attcagcgag atcgccttta acgagatcgg taatctcggc gagtagttcg tcaacggcga
      181 acggcttggt cttgaaacgc gccgcgccaa gcgacatgac tttggtgtgc ttaagttctt
      241 catcggtcaa actgccggtg atcaccatta ccggaatgtc agccgtatct tccgagttct
      301 ttagcttctg gagcacttca taaccatcca tcccgggcaa tttgatgtcc agcaaaataa
      361 gatcaggtat ttcttgacgc gccagcacta atgcgcgctt accgcttgag gtcagcacga
      421 cgctgaagcc gcgcgcttcc aaggcggtct gtaagaagtc gcgtgtgtct cgatcgtcgt
      481 cgacaaccag cactttaccc ttgccaaaca aaatacgatt gatcgatgca actagccggg
      541 acggatcgat gggtttagtg agatagtcga ccgcacccag ccggaagcct tcgtgtcgat
      601 ccggcatgac cgaaacaata acaatcggaa tatcagccgt cgccggattg gtcttgagtt
      661 cttgcaacac ttcaaaaccg tctcgatccg gtaaatagat atcgagcgtg atcaaatccg
      721 gctgatcgat ttgagcttgc tccagcgcgt cgcgggcgcg gctggcaatc cgcacatcgt
      781 aacctacact ggtcaaatgc tgatggatta actcggcaat gtcatgatca tcttcgacaa
      841 ccagaatacg ccgcccgccc ccgtttgatc gcgccacccg atcgatctgg cgcgccagtg
      901 gcctgaattc gacaggttca accggtatca attcttcaac tgattcaacc actgcaatct
      961 tcaaagtaaa tgagaagata gtgcctacat ccggctcact ctccagccag atgcggccct
     1021 catgcatctc caccagtgat ttcacaatcg ccagacctaa accggtccca ggaaattcct
     1081 gcacgaccgg atcatcggcc cggaaaaatc gatcgaatac tttcatatga ctttccggtg
     1141 agatgccaat accggtatcc gcgacatcga tctggacgaa gtcatcgacc ggccgcgccc
     1201 gcaccgtgat cgatccgccc gcaggtgtat attgatacgc gttgctgact aagttggtta
     1261 aaatctgaat cacccgatcc cgatcaccag ttacatccgg cagatcgcgt tcaggcacat
     1321 ccgtaatcag atgtacgccc ttctcttcaa tcttgccgcg cagcgccgtc aatgattgtt
     1381 ccacaaccga ttcaaccgac agcaccttga agtctaactt cacacgtcca ctttcaatcc
     1441 ggctgatgtc cagcaagtca ttcaacaaga cagacaatcg atctgcattt gacttgataa
     1501 cactcaagaa acggtgctga ttttcattga ccctcccagc tgcgccgaac agcagtagat
     1561 ctgcgtaacc tttgatgcta gtcatcggtg tacgcagctc gtggctgacg gtcgatacaa
     1621 actcggtctt agcgcgatcg gcttcaacct cgcgtgtgat gtcccggaag actgacactg
     1681 aaccgagata ctcatcgcct aaggccaccg gcgccaacgt gacattcaca aaacgactct
     1741 ccggcaaaaa ctccatgagt tgtgacaata cgggcgattt gcggcgtgcc tcgatcgatc
     1801 ccaaccaagc acgcacttct gccacaaatt gtgggccagc tgccgaatat aagcccagca
     1861 tatctgcgat cgagcgggcc ataaccttat cgcgcttgac atccaagata cgctcggccg
     1921 cggcattgaa caagatcacc acacccctcg cgtccgtcac caacacacca tccgtcaccg
     1981 attccagaat ggcctggttc ttggaacctt cgacttgttg cgcgcgcagc ataccgccca
     2041 gcagttcggc ttgttcgcga atgtagcgat acaactcggc gttgttaatg gccgtcgcca
     2101 cctgcgtcgc cgccgcggcc accagacgcg agtgggattc ggtaaaataa tcgggctgcg
     2161 gatgcagcaa caaaactgaa cccaacacat cttcaccaat aatcaagggc gcggccagcg
     2221 ccgatcgata ctcgcgatcg gactcgcgtc gttcggccca gcgtcgatct tgttggatgt
     2281 ccggcacaat caccggctgc cgattcacta ccgaccagcc cgccaggccc tcgccttttc
     2341 ggaacgctgt cggcttaccg cccagcggca gtgggtcttt gtcgcctaac acagcgcgca
     2401 atgtcaaagt gtctttctgc ggatcgacga gcaagatcga tccataaggt gcggcaacgg
     2461 cagccaccat caattccaat gctcgattaa gaacccgatc gagatccaag ctggccgata
     2521 gttcagatgt gatcctaaag agcatctcca cccgatcccg ttcagtcgtc aattgagcat
     2581 tggcgcggcc gagctcccgc gtgcgttctt cgacgcggcc ctccaattcc tgcgtgaact
     2641 tgaccacttg atcatacaag cgcgcattgc tcatagcaac cgcgccttga ttaaccagcg
     2701 tctccattag ttggatttca ctgtccgtca agatgcgatc gaaacgacca tcgcccaccc
     2761 gcaccagacc caacacctcg ccgcgcgcca cgatcggcac ttccaacaac cacaataagc
     2821 cggcttgccg catacgatct ttcaaagaag gatcgagctc cggatcattt agccgcaggc
     2881 tcaagctgcg tcgatcttgc aggaattgcg ctaccttcaa atagtccagc ggcgcaaaag
     2941 tatccgtcgc taattcagat tgctttaacc cttccggcgt caagtaacgg gtatgcactg
     3001 tcgtaagttt gtcgttgaca cgatcccatt cttggatgat caccatatgt gcgctcgtcg
     3061 cgcgcagtaa ctgactgctc accgtttcca gcacttggtt gtaattcaag gtcgatgaga
     3121 tcgcctgacc ggcctcatac aacaacgatt gctcgcgcaa ccgccgttga gtctcttcgt
     3181 ataatcgggc gttctcgatc gccactgcgg cctgatcggc caatgtcgac aacaccacca
     3241 agtccgtatt actaaagccg ttcggggtgg tcttatcgac caccaataca cccataactt
     3301 gagcggcgac gatcagcggc actaacacca ttgacctcac atcggctgca accggcactt
     3361 cgacaaagcg atcatcttga tctacatccg gcacgatcaa gccacgctgt tcggccacca
     3421 ccgatccgac taaaccttcg ccgctggcaa accgcaacga acgtgcttca gtcagatcaa
     3481 aaccaatcgc tttgcgcggt gcatattttc catcgagcgg atcgcgcatg aaaaccgtgg
     3541 cgcgcgaggc ctgcatcaac tccaccgagg catccaacac cgtcgtcaac aacgattcca
     3601 gatcgagctt ggcactgatc gatcggccta cttcattaaa cagggtcaag ttatcgagtc
     3661 tgtgttgcaa gtctgcatac agctgcccat tctcaacggc aatcgccgct tgatttgcga
     3721 acaattccag cgcttcaatc gtcgcccgat caggaaggcg gccgcttgcc ggttcatcga
     3781 cggacaggag acctagaatg cgatcgcctg atccacgcag cggtgtgatc aataaatctt
     3841 ccggatgcca catgcccggc acacgcacca acgaaagatc accgcctggc gccatataga
     3901 catccagcgt gtccgccgct tcgcgcttct ccagcggaac gtaataactt tggctgatca
     3961 ggaattcatc ctgcataatg gcttcgatgt ttttccaggg ctgctcgact tgacgcatct
     4021 tctcgaaagt tgccatggga atcccagcag cagcttcgcg ctttaagcgc ggtggttcac
     4081 ctgtcagaac gcttaggacc acaatattga aacccaccgt ctcttgaatc gcgtgcacca
     4141 cgtcatcaaa gattttctcc agcggttgat ccgtgcgaaa ggcctgaccg atctggaaga
     4201 gattcgtcaa ttgctcggcg cgacgacgca gcaagtcact ctgcacgagc tgctcttcca
     4261 gtcgctgcgc gttgccaatg gcaatggcgg cctgagaagc caacgccttt aggaattcca
     4321 gatgatccgg tgtgaacgca tgaagctgcg tactctccag attcaatacg ccgacgacag
     4381 attgtgcgta tcgaattggc accacaatct ctgagcgagt atctggacta atcttcatat
     4441 aatttggatc gccacttaca tcattaacaa taatcggctt gccctccgct gctgctcgac
     4501 cgctcaagcc ggtgcccagc ggaatgcgaa tcgattgtat tcgcagcgct tcatcacgct
     4561 cgaacccaag cgatgcacgc acttccaacg actgactgtc gacgccaacc aaactcacaa
     4621 tgccatactg tgcgcctgtc acgcgtgcgg cttcatccaa gacaagtgag aaaatgtgat
     4681 cctgatccag cgtcgaattc aactcttgcg tgatcgccga caacgcagtg agctcttcca
     4741 cacgctggcc caggcggaca tcggttaatt gatacagccg cgcattctcc aacgccccgc
     4801 ccgccgagtt ggcaatcgac tgcaataagt tcaaatcatt ttgatcatac gcaaatcgat
     4861 cgtgaaaatg ctggaccgaa atgattccgg cgattcggtc gccgatgatc atgggcacac
     4921 ccatccacgc aacggctggc tggccagaag cgataagacg catctgaata cccaagcgcg
     4981 ccatttcggc attgagatct ccacgcagca gcagcgcttt acgctgatga cagacaaaga
     5041 aggtcagcgt attttcaccg cgccgtttgt tatcttgacc cagcggcgtc agggcaccct
     5101 cttcgtaaat gcgtgcaaat gtcacttcgt cttgcggttc gtcatagtag gccacatata
     5161 atgtgcgcgc atccatgaca cggttggtct gttggtatag gatatccaaa acttgatctt
     5221 catttaaagc ggccgacata gcacggccga tctcacttaa gtaactgagc tgctcaatgc
     5281 ggcgctgcga ttcttcatac aaacgcgcat tctcaatggc attcgccaac agcgccatca
     5341 ccgtcgataa tcgtcgctca tcgtcatgcg taaatggtac gttatcgcgt ttgacaatat
     5401 agacttcgcc aatcgcctgg tcatgtgtga ccaacggacc gatcagcaga ccccgggctt
     5461 gaaagtgctg cagcaacggc agatatgtgg gcgcaatccg gcgatcaatc agagcattat
     5521 cgaacctccg tacctgacga gtggcaaatg cgctgcgatg gaacgacggg cgggttgtat
     5581 ctatctggaa atcacccacc gtatccgcca aatcaccgta ggcagtggac aataacggct
     5641 ccaattgttt ggtgtccgcg ttatacaaca tcaccaaacc caattgtgcc ttcaacaaag
     5701 tgcaaatttc agccatcaag ttctgcagca acttagccgg ggacgaacct gaagcagcaa
     5761 ccgccgaaat acggaacaaa ctggctgact cgtcagcacg atgatgtgct tcttcaaaca
     5821 acaaggcgtt ttgaattgcg atcgccgcct gacccgcaat ggttcgagtc gtttccaagt
     5881 tagcggccgt aaacgcactc ggcgtgtagg aggctagctc caaagtgccg atcaacgtat
     5941 cgccggagat tagcgggaca ccgcaatgcg atcggatcgg gaactgctcc cgatcgatca
     6001 tcggcagcac atcgctctga gcaacgtcat cgatcaacag gggcagccga ttgcgagcca
     6061 accagccggt tagtccttga tcaatatgat aaacgccgcc ccggatttgg ctatacgcgg
     6121 catatttcgg atctgcgccg cggccatggg tgcgcatcac accttccgtg gcatcccata
     6181 aattgatctc ggccagatcg tatggaatca aagacttgac cgaacccaag attgcgtcga
     6241 gtgttatatc caacgccagc gatgaactga tcgtccggtt gatctcggcg atcactgaca
     6301 tttcctgagc gcggcggcgc tcgacttcat ataactgcgc attcttaatt gctgctgcag
     6361 cttgatccgc aaagttattg agtaaccgct gatcatcgcg gctgaatgac ggtgtgccgg
     6421 ggcgcgtcgc aacttgcagt accccgacca ccacctcatc gattttcaac ggtacattca
     6481 tcacagcggc aatgtcatga tcatgcgccc atttaataat gaacctagga tcagccggtg
     6541 cattcgcaag aaacaatgat cggttttcct gcgccaccca cccgataatg ccttcaccta
     6601 gcgagtataa caacatcggc tgcggaacac cttcaaaatc ccaccagcct cgcaaggtca
     6661 acacctgcct gcgcggatcg atcacaaaga ttgagcaccg gtcagcgccg acagcgccgg
     6721 ccgattcgcg ggcgaggttg ttcagtacgc tttccagatc gagcgaagta atggctcgtc
     6781 cggtttgaag cagtgtggac atttctttga cgcgccgctg caccacttca aattgctgtt
     6841 gccgctcaag tgccacactg gcggaattgc tctgggcttc ttgcagcttg gtcgccatct
     6901 cattaaattc tg
//
LOCUS       DASRTJ010000251         7615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_157533,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000251 DASRTJ010000000
VERSION     DASRTJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157533"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /gene="gatC"
                     /locus_tag="VFF70_05215"
     CDS             complement(<1..201)
                     /gene="gatC"
                     /locus_tag="VFF70_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZY44134.1"
                     /translation="MPLTREEVQHIAELAKLQLSAEEETRYQEQLSAILDYAARLNAL
                     DVESIPATASVLPLRSIMREDVA"
     gene            335..1048
                     /locus_tag="VFF70_05220"
     CDS             335..1048
                     /locus_tag="VFF70_05220"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HZY44135.1"
                     /translation="MENRNQRGKLADRIRVINRFLIDHHLYPLLLSSALACGLIVGRV
                     TVYRTWMYAFLIWNLVLAWVPYAWSLWAASIHRRFPRRWWPLLMPGVVWLIFFPNAPY
                     IVTDLLHLHDHAPVPLWYDAGLLAAFAFAGIFIAISSLHTMQSIVEDLANRTIGWLFA
                     IGIIGLTGFGVYLGRFLRWNTWDLFLNPIDVLGDAAHRLAHPLSNLQTYGFTLLFAAF
                     LFGCYWMFTSMQPRTQESD"
     gene            complement(1133..1633)
                     /locus_tag="VFF70_05225"
     CDS             complement(1133..1633)
                     /locus_tag="VFF70_05225"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZY44136.1"
                     /translation="MQFKAGDYVVHPSYGVGSVVRLEEKRLAEADTRLYYVIDVEKST
                     VWVPVDQHGTLELRKVTPSQDLEQYRRLLKGKPVMLDKDHHRRRLLITERMRPGSFRV
                     MCEIVRDLTAYGWNKPLSDVDASSLQKIRSGLCREWAAAAGVTPQQANQEVEALLLAG
                     RQAHKV"
     gene            complement(1712..2065)
                     /locus_tag="VFF70_05230"
     CDS             complement(1712..2065)
                     /locus_tag="VFF70_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZY44137.1"
                     /translation="MEVKLYVGNLSYSTTEDELRTLFAQAGSVASVALIKDRDTGSSK
                     GFAFIEMTTQAEAQKAISMFNEYKMGERALTVNLARPREERSGGGGGRGGFGGGNRGG
                     GGGGGGNKGRGGSRW"
     gene            complement(2189..3202)
                     /locus_tag="VFF70_05235"
     CDS             complement(2189..3202)
                     /locus_tag="VFF70_05235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZY44138.1"
                     /translation="MKLDASIGFDTALKDIPSIAQAAESIGFDAIWTSETQHDPFLPL
                     TLIAEHTARMKFGTAIAIAFARSPMIVAHTSFDLARQSNGRFILGLGTQVKAHIERRF
                     GMTWDAPVPRLREYIQALRSIWNSWQTGSKLNFRGEHYKLTLMNQFFTPGPIEQPDIP
                     IFIAGVGEPLCKLAGELADGFHVHPYHTRKYLSEIILPAIDAGLRKSDRARGSIEIAS
                     MVFVALDDQEIEAQRRQISFYASTPSYRPVLELHGWGSISDQLGALAARGRWDEMPKL
                     ISDEMLETFVIIGSWEDVAAKLRQKYAGSLNRVGLYRPYVPGVEDDRWRQLTQQLLDL
                     GKA"
     gene            complement(3297..4259)
                     /locus_tag="VFF70_05240"
     CDS             complement(3297..4259)
                     /locus_tag="VFF70_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY44139.1"
                     /translation="MDYKNLGRTGLKVSELCMGSMNFGWTAPRENAVPVFDAFVEAGG
                     NFIDTANVYSRWAPNGYAGLAEELIGLWLKTKPRDQIILATKVRGKMGDGPNDQGLSR
                     KHIIDACEASLRRLQTDYIDLYQTHFWDEHTPLDETLHALDDLVRSGKVRYIGASNLP
                     AWHLMESLWISDRQNLTRYDTLQPHYNLIERAEFERELQTVCLKYGLGVIPYSPIAGG
                     MLSGKYRRGTVATEGTRGWNLRHAFTERNFQIIDKLDEVAQAQSKTVLQVALAWVMLQ
                     PAITSAIIGANTIEQWTELAGAIGLKLSEDELKQLNEVSKWEEG"
     gene            complement(4371..5348)
                     /locus_tag="VFF70_05245"
     CDS             complement(4371..5348)
                     /locus_tag="VFF70_05245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY44140.1"
                     /translation="MRALITGGTGFLGANLAEGLIKQDWRVRILRRTSSPLDAVKDLK
                     VEHAIGDVLDLQSLLAAMKDVDIVFNVAAISQYWRTKPDLIYQVNVEGASNVFDAAQQ
                     STVKRVVHTSTGAAVGIIKGRLSNENDPYNLPIKRFAYGHSKWLAEQLVRRRVKSGQD
                     ITMVNPTGIIGRRDINWISGSILREAARHRVPVLAQGGVNFVAVQDVVAGHIAAVDKG
                     RAGERYILGGENLLNRDTLLMACEIAGVNPPRFMQPRWSIPIVAAILDLIAAIIGPRL
                     PISGEQMRLTREYLWFDSSKAQKELGLPHTSVRQAMQECYDWYKRNNYL"
     gene            complement(5381..5785)
                     /locus_tag="VFF70_05250"
     CDS             complement(5381..5785)
                     /locus_tag="VFF70_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HZY44141.1"
                     /translation="MNTIYAVKVEVDPVDEATWNEWNTTLHIPDVLMQRGFLRASKYK
                     IDSDAEWPQYLILYELESRAALDNYLKGDAVIRLRADHYSRFGSSTRLSRMLLTPIAF
                     IEKDKPTPRKKPVPARLKSTLKKAAPKKRARK"
     gene            complement(5801..6148)
                     /locus_tag="VFF70_05255"
     CDS             complement(5801..6148)
                     /locus_tag="VFF70_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44142.1"
                     /translation="MFFSITFLGWICLAAAILLTFFYPLGQLHRSHVVSQAAMFICYV
                     MAAFILIPYEWVPQDLGWKALIIGAIVGLLAIIFRDIRRFFRFFHGKIYRMTHPYYWY
                     SRAFRSLSRRQSR"
     gene            complement(6175..7077)
                     /locus_tag="VFF70_05260"
     CDS             complement(6175..7077)
                     /locus_tag="VFF70_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HZY44143.1"
                     /translation="MHKTLDALIRLTRYKEYLWFVVVTTLLGVAAANGTFSLKLIIVL
                     IANWLAVGFSFMINDVEDAADDALNPAKVNRNPVSAKILSPRTAYVASFAVMILSGVM
                     YALLGTLPFIIGLISLTVGFLYSWQRVRLKSMPFVDLITHCMMLAGFQFLPAYFTFTS
                     TLNDRWLWPFLAVTSISMYGELFNELRDLTGDLEAGVKHTASVLGQRATQILANILLA
                     LGVIALFITLFFVALIPVWVLIVMALLALILIVPPLLRVRRSQNMVQLQGSFQVPLTI
                     AAAAALVIGYLAPWLNSFFHFGIF"
     gene            complement(7152..>7615)
                     /locus_tag="VFF70_05265"
     CDS             complement(7152..>7615)
                     /locus_tag="VFF70_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZY44144.1"
                     /translation="VTAGNSSGLNDGASAVLIMSADRAKDMGLKPLARIVACAAVGVD
                     PRVMGYGPIPSTRKALKRAGLTIDQIDLIELNEAFAVQSIACARELGIPNDKLNVNGG
                     AIALGHPLGCSGARIVTTLVHEMKKRGSRYGLATMCVGVGQGEAMIVEMIK"
BASE COUNT         1739 a         2152 c         1909 g         1815 t
ORIGIN      
        1 agctacatcc tcgcgcatga ttgatcgtag cggcagaaca ctggccgtcg ctgggatcga
       61 ttcgacatcc agcgcattca accgcgcggc gtaatccaaa attgctgaga gctgctcttg
      121 ataccgcgtt tcttcctcgg cgctcaattg cagtttagcg agttcggcaa tgtgttgaac
      181 ttcttcgcgc gtgagtggca tacatcgatt ataacagagc ggctgatcaa tgacaactga
      241 tcgatcaaaa aacggtaaat tcccattgca cttaagtgct tgattcttac tgtgggatcc
      301 gttagagtag acgcaagccg ttggaggttt gttaatggaa aatagaaatc aaagaggcaa
      361 gctggccgat cggatacggg tgatcaatcg gtttttgatc gatcaccatt tatatccact
      421 gttgctatcg agcgcattgg cgtgtggcct gatcgttggc cgagtgactg tctatcgaac
      481 ctggatgtac gcatttttga tctggaatct ggttctagcc tgggtgccgt atgcatggag
      541 cctgtgggcg gccagcatcc atcgacgctt tccacgcaga tggtggccac tgctgatgcc
      601 gggcgtcgtg tggttgatct tctttccgaa cgcgccgtac atcgtaaccg atctgctgca
      661 cttacacgat cacgcgcccg tgccactctg gtatgacgcc ggcttgttgg cggcatttgc
      721 tttcgccggg atttttattg cgattagctc actccatacg atgcaatcga tcgtcgagga
      781 tctggctaac cgcacgatcg gctggctgtt tgcgatcggg atcattggct tgaccggttt
      841 cggcgtctat ctcggtcgat tcttgcgctg gaatacttgg gatttgtttc tgaatccgat
      901 cgatgtgcta ggcgatgcgg cccaccgctt ggcgcatcca ttgagcaatt tgcagaccta
      961 cggctttacg cttttattcg cagccttctt atttggctgc tactggatgt tcacttcgat
     1021 gcagccccgc acccaagaat ctgattaact ttgcggacaa caaaagtgct ccgcctcaaa
     1081 tgcgaggtgg agcactttat tttgagttga acgatcgggt tcagcgcagg ctttagacct
     1141 tgtgcgcttg tcgaccggcc agcagtaagg cttcaacttc ctgattggct tgctgcggcg
     1201 tcacgcctgc ggcggccgcc cattcgcgac acagccctga gcgaattttt tgcagcgaag
     1261 acgcatcgac atcactcagc ggcttgttcc agccgtaggc cgtcagatcg cgcacgattt
     1321 cgcacatgac gcgaaacgaa cccggtctca tgcgctccgt aatgagcagc cgtcggcggt
     1381 gatgatcttt atccagcatc accggtttgc cttttaacag gcggcgatac tgttcaagat
     1441 cttgactggg cgttactttg cgcagctcga gggtgccatg ctgatcgacc ggcacccaca
     1501 cagtgctctt ctcgacatcg atcacatagt acagccgcgt atccgcttca gccagtcgct
     1561 tctcttccaa tcgcacgaca ctacccactc catacgatgg atgtacgacg tagtcgccag
     1621 ctttgaattg catcaggcgc tccttccgcg gtttactgcg tttggtggac atgatcaata
     1681 caggcccact atatcggatt tgtcggtgct tttaccagcg cgagccgccg cggcccttgt
     1741 tgccaccgcc gccgccaccg ccgccgcgat tgccgccacc gaagccacca cgaccaccgc
     1801 caccgccgct gcgctcttca cgcggtcgag cgaggttcac ggttagcgcg cgttcgccca
     1861 tcttatattc attgaacatg ctgatcgcct tctgcgcctc agcctgtgtc gtcatctcaa
     1921 tgaacgcaaa gcccttagag ctgccggtgt ctcgatcttt gattaacgcg accgaggcca
     1981 cgctaccggc ttgcgcaaac agcgtgcgca gttcatcttc ggtggtcgaa tacgacaaat
     2041 ttccaacata caacttgact tccatggtta tctctccttg agatatcggc ctcacagccg
     2101 aaaataaaaa gccgccgatc cataagaggt tccggcggcc aacttcgcac acacatccta
     2161 ttacacgtgg gcgtcattat accacacatt aagctttgcc aaggtccagc agctgttggg
     2221 tcaattgccg ccagcgatca tcttcaacgc ctggcacata tggtcgatac aaaccgaccc
     2281 gattcaacga gccggcatat ttctggcgca gtttagcggc cacatcctcc cacgagccga
     2341 taatcacaaa cgtctccaac atctcatcgc tgatcaactt cggcatctcg tcccatctcc
     2401 ctcgcgcggc cagagcgccc aattgatcgc taatcgatcc ccagccgtgc agctccaaga
     2461 cgggtcgata cgatggcgtc gaggcgtaga acgatatttg tctgcgctga gcttcgatct
     2521 cttgatcgtc caacgccaca aagaccatgc tggcaatctc gatcgatcct ctggcccgat
     2581 cggactttcg caaacccgcg tcgatcgcgg gcaagatgat ctcgctcaaa tatttgcggg
     2641 tgtgatacgg atgcacatga aagccatcgg cgagttcgcc ggccagtttg cacaaaggct
     2701 cccctactcc ggcgataaag atcggaatat ccggctgctc gatcggcccg ggggtgaaga
     2761 actgattcat caacgtcagt ttgtaatgct cgccgcgaaa gttgagcttc gatccggtct
     2821 gccacgaatt ccagatcgat cgcagcgcct ggatgtattc acgcaagcgc ggcacgggcg
     2881 cgtcccacgt catgccgaac cgccgctcaa tgtgcgcttt cacctgcgtg cccagcccca
     2941 aaataaagcg gccgttggat tgtcgagcga gatcgaacga tgtatgcgcg acgatcatcg
     3001 ggctgcgcgc aaacgcaatc gcgatcgctg tgccgaactt catacgcgca gtgtgctcgg
     3061 cgatcaatgt cagcggcagg aatggatcgt gctgagtctc gcttgtccaa atcgcgtcaa
     3121 agccgatcga ttcggcggcc tgggcgattg acggaatatc tttcagcgca gtatcaaatc
     3181 caattgaagc atcaagtttc atattgataa caagtaacac ttgcaccgca ctacgtgcgt
     3241 tacagtgcag gtgtgagtaa cgagtaagaa tcaatttact cgttaattgt tactcattaa
     3301 ccctcttccc acttactcac ttcattcaac tgctttaact catcttcact caacttcaaa
     3361 ccgatcgcgc ccgccagttc tgtccattgc tcaatggtgt tcgcgccaat aatcgcactg
     3421 gtgatcgctg gctgcagcat gacccaggcc agtgcgactt gaagcacagt tttactctgc
     3481 gcctgggcaa cttcatcaag tttatcaatg atctggaagt tgcgctcagt aaacgcatga
     3541 cgcagattcc agccgcgtgt gccctctgtc gccacagtgc cacgtcgata cttgccgctc
     3601 aacatgccgc cggcgatcgg cgaatacgga atcacgccga ggccgtactt taagcacacc
     3661 gtctgaagtt cgcgctcgaa ctccgcccgt tcgatcaagt tgtagtgcgg ctgcaaagta
     3721 tcatagcgtg tcaagttttg tcgatcgctg atccataatg actccatcaa atgccacgcg
     3781 ggcaaattcg atgcgccgat gtaacgcact tttcccgatc gaaccagatc gtccagcgcg
     3841 tgcagcgttt catcgagcgg tgtgtgttca tcccagaaat gtgtttgata cagatcgatg
     3901 taatccgttt gcaatcgccg cagtgaagcc tcgcacgcat cgataatgtg tttacgactc
     3961 aagccttgat cgttcggacc atcgcccatc ttgccgcgca ctttggtagc caggatgatc
     4021 tgatcgcgcg gctttgtttt caaccacagg ccgatcagtt cttcggcgag gccggcataa
     4081 ccattgggcg cccaacgcga atatacgtta gcggtatcga tgaaattgcc gccggcctcg
     4141 acaaacgcat caaacaccgg cacggcattt tcgcgtggcg ccgtccagcc aaaattcatg
     4201 ctgcccatac agagctcgct tactttcaaa cccgttctgc caagattctt gtagtccatg
     4261 ctaatctctg atctctgaat ttagatttct gatttgttcg ctaacattga gcattgttca
     4321 ttgaaaattg atcattgcca atttgtcatt gggcattttt catttgtcat ttacaaatag
     4381 ttattccgct tgtaccaatc atagcattct tgcattgcct gtcgcacgga agtatgcggc
     4441 aagccgagtt ctttttgcgc cttcgacgaa tcgaaccaca ggtattcgcg cgtgaggcgc
     4501 atctgttcgc cgctgatcgg caatcggggt ccgatgattg ctgcgatcag atcgagtatc
     4561 gccgccacaa tcgggatcga ccagcgcggc tgcataaaac gcggcggatt cactccggcg
     4621 atttcgcaag ccattaacaa tgtatcgcgg ttcaataaat tttcgccgcc gagaatgtag
     4681 cgttcaccgg cgcgcccttt gtccactgca gcgatgtgcc ccgccaccac atcttgtacc
     4741 gcgacaaagt tgacaccgcc ctgtgctaat accggcacgc gatgacgcgc ggcttcgcgc
     4801 aaaatgctgc cgctgatcca attgatatcc cgccgcccga tgatgcccgt gggattcacc
     4861 atcgtaatat cctgcccgga ctttactcgc ctgcgcacca attgttctgc cagccatttg
     4921 ctgtgcccat atgcaaatct ttttatcggc agattgtatg gatcgttttc atttgacaga
     4981 cggcctttaa taatgcccac cgccgcgcca gtgctggtat gcacaacgcg tttcacggtc
     5041 gattgctgcg ccgcatcaaa cacattgctc gcaccttcga cattgacttg gtaaatcaga
     5101 tcgggtttgg tgcgccaata ctgtgagatg gcggcaacat tgaagacgat atccacatct
     5161 ttcatcgccg caagcagcga ttgaagatcc agcacatcgc cgatcgcgtg ttcaactttc
     5221 aagtctttca cagcatcaag cggcgaggac gtgcggcgca atatacgcac tcgccaatct
     5281 tgcttgatta agccttcagc caagttcgcg ccgaggaagc ccgttccacc cgtgatgagt
     5341 gctcgcataa tttatcgttc gaaaggctgg tttacgcccg tcattttcga gcgcgcttct
     5401 tgggtgcagc ctttttcaaa gtcgatttca atcgagccgg aacaggtttc ttgcgcggtg
     5461 tcggtttatc tttttcaatg aacgcgatcg gcgtcagcag catccgcgac aggcgcgtcg
     5521 atgaaccgaa gcgcgaatag tgatcggcgc gcaatcggat gacggcatcg ccttttaagt
     5581 aattatccag cgcagcgcgc gactcgagct catacaagat aagatattgc ggccactcgg
     5641 catccgaatc gatcttgtat ttgctcgcgc gcaggaatcc ccgctgcatc aacacatcgg
     5701 ggatgtgcag cgtcgtattc cattcgttcc aggtcgcttc atcgaccgga tcgacttcaa
     5761 ctttgacggc atagatggta ttcatgatcg atcttctcct ttaacgagat tgccgacggg
     5821 atagacttcg aaaagcgcgg ctgtaccaat aatacggatg cgtcatgcga taaattttgc
     5881 cgtgaaagaa tcggaaaaaa cggcggatgt cgcggaagat gatagcaagc agaccgacga
     5941 tggcgccgat gatcaatgct ttccatccta gatcctgcgg cacccattcg tatgggatca
     6001 agatgaaggc cgccatcaca taacagataa acatcgccgc ttggctgaca acgtgactgc
     6061 gatgcagctg gcccagtgga tagaagaaag tcaacaagat cgccgcagcc aagcaaatcc
     6121 aacctaagaa tgtgatggag aaaaacatat tcttcctcgc ctgatcgatc gagtttagaa
     6181 aatgccgaaa tgaaaaaacg aattcaacca tggtgcaagg tagccgatca ctagcgcagc
     6241 cgcagcggcg atcgtcaggg gcacctgaaa tgatccttgc agctgcacca tgttttgcga
     6301 tcggcgtacg cgcagcaatg gcggcacaat caatatcaag gcgagcagcg ccatgacgat
     6361 caatacccag accggaatga gcgcgacgaa gaagagcgtg atgaacaagg cgatgacgcc
     6421 taacgctaag agaatgttcg ccagaatttg tgtggcgcgt tggcctagga cactggctgt
     6481 gtgcttgacg cccgcttcta gatcacccgt gagatcacgc agctcgttga acaattcgcc
     6541 gtacatgctg atcgaggtca cggccaggaa cggccacagc cagcgatcgt tcaacgtcga
     6601 ggtgaacgtg aagtaggcgg gtaaaaattg aaagccagcc agcatcatgc agtgtgtgat
     6661 cagatcgacg aacggcatcg atttcaatcg aacgcgctgc catgaatata agaagccgac
     6721 ggtcaaactg atcagcccga tgataaacgg cagcgtgccc agcagcgcgt acatgacgcc
     6781 cgataggatc attacggcga acgaggcgac atatgctgtg cgcggcgaca atatttttgc
     6841 ggacaccgga ttccggttga ccttggctgg attcagcgca tcgtccgcgg cgtcttcgac
     6901 atcgttgatc atgaacgaga aaccgaccgc cagccagttt gcgatcagca cgatgatcaa
     6961 tttcagacta aacgtgccat tggccgctgc cacaccgagt aaggttgtga cgacaacaaa
     7021 ccacaggtat tctttgtaac gagttaaacg gattaaagca tcaagtgttt tgtgcatcag
     7081 agcctttcaa attttagcaa ctaacgaagt aggtttgtca tttgttattg tccattgttc
     7141 attgcacatt gctacttgat catttccaca atcatcgctt cgccctgccc cacgccgacg
     7201 cacatcgtag caagaccata acgcgagcca cgcttcttca tctcatgcac caacgtcgtc
     7261 acgatgcgcg cgcctgaaca gccgagtgga tgtcctaacg cgatcgcgcc accattgacg
     7321 ttgagcttgt catttggaat gccgagttcg cgcgcacacg cgatcgattg cacggcgaag
     7381 gcttcattca attcaatcag atcgatctga tcgatcgtca ggccggcgcg cttcaatgct
     7441 ttgcgcgtcg agggaatcgg gccataaccc atcacgcgcg gatcgacgcc aacagctgcg
     7501 caagcgacaa tgcgtgcgag cggcttgagc cccatgtcct tcgctcgatc ggcgctcatg
     7561 atcaacactg ccgatgcgcc atcattcaag cccgatgaat taccggcagt caccg
//
LOCUS       DASRTJ010000252         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_195456,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000252 DASRTJ010000000
VERSION     DASRTJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195456"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFF70_05270"
     CDS             <1..299
                     /locus_tag="VFF70_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZY44145.1"
                     /translation="QNAVLQPSSQNVIDFVANNPGAIGYASISDADSKVKVLPIEGVT
                     PNPTTAASNQYLLSAPIYLIAKSEPSGDLREFVAWLLSDPAQKLISQSGLGRVR"
     gene            complement(396..3038)
                     /locus_tag="VFF70_05275"
     CDS             complement(396..3038)
                     /locus_tag="VFF70_05275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY44146.1"
                     /translation="MSDDPLIGKQLGPYKILEPIGQGGMATVYKATQPSMNRTVAVKI
                     LSPLMASNATFLARFKQEAQMIASLEHAHILPVYDLGEQDGIVYIAMRYMGFGSLQSR
                     MAAGMVALRDVARWLEQVASALDYAHERSVIHRDVKPSNVLIDQQGNAFLADFGIAKW
                     MEGSIGLTGSSVIGTPQYMSPEQGQGIKIDGRSDEYSLGVMAYEMIVGRPPFEAETPL
                     GIVLKHVTEPLTPPKMINPRIAESISDVVAKSLSKSPDNRYPTCGGFAEALADAIRSA
                     PPPSTEPLRSTAAPTEQVHLTKTHPRSIGLSRSIVIALIMLGLIVVGVGGAIVLSAVQ
                     PPPDRPLVVPTIGSNIQILTPTPPSSIVTPQATTSSVTNNVAACTPIYSENFDAPVNF
                     PSGEQEGATWGVADGEYRLSIKSANFFQSQLIGPNLKDYQVDVDAQFNTDAIGTYGLV
                     VAARGQDDYIAFVVDANRNFMITRRTSNGSTPIQDSTFAPALKPGQAINYLRVIQRGQ
                     QIALYANDVLLKLIEDVGDPNSDKRIGLIASSFNRGGVDARFDNVIVCSAPETFAAHE
                     VALIDSFDDNRNGWAPQQYTSNGTSTIENGKFVIEAVYPGKAYGVSTWNPNVAFNNFD
                     LNVDAQILTGTGTSQLGILFGVQDINNAYLFRVINDGRYQLYHIVGGSYQALADAQSS
                     PAMNADQALNHVHVSMLTGTLTIDVNDQQIMQAQVAYAAGFVGFWCGVDAPTRTRCGF
                     DNLVVQGTPSTSALTVYPFCNCVREARVGQPLSVSWTWGAKTQNYLDALQAKSILTVT
                     LDGLPIDQPKQYWGNPQIDQDGISLHWSYNLPALDPGSHVLEFTVHVDEAVTDGFDRN
                     GDGKPDTYGPGDFISGYVNVVVLP"
BASE COUNT          758 a          893 c          841 g          715 t
ORIGIN      
        1 cacaaaacgc ggtcttgcag ccatcgtcac aaaatgtgat cgacttcgtg gcgaacaatc
       61 ccggcgcaat cggctacgca tcgatcagcg atgccgattc gaaagtcaag gtacttccaa
      121 tcgaaggcgt gacgccgaat ccaaccaccg ctgcatcgaa tcaatatttg ttatcggcac
      181 cgatctatct catcgccaaa tctgaaccga gtggcgatct gcgcgaattc gtcgcgtggc
      241 tattgagtga tcccgcgcaa aagttgatca gccaatcagg cttaggcaga gtccgctaaa
      301 actttcgggc ataacaaaaa gaggcgatca gccctctcca cttcgttacg agggtcttcg
      361 accgagcgcc cctccacgaa cttccacttc ctaattcacg gcaacactac tacattcaca
      421 tagcccgaaa tgaaatcgcc gggaccgtaa gtgtccggct tgccgtcgcc gtttcgatcg
      481 aagccatcgg tcacggcttc atcgacatgc acggtgaact ctaatacatg actgcccgga
      541 tcgagcgcag gcaagttata tgaccaatga agtgagatgc catcttgatc gatctgcgga
      601 ttgccccaat attgtttcgg ttgatcgatc ggcagtccgt ccaacgtcac ggtcaagatc
      661 gatttggctt gcagcgcgtc caaataattt tgcgtcttcg cgccccacgt ccacgacacc
      721 gacagcggct ggccaacccg cgcttcacgc acgcaattgc agaacggata aaccgtcagc
      781 gcgctggtcg agggcgtgcc ctgcacaacc agattatcga agccgcagcg cgtgcgcgtg
      841 ggcgcatcga cgccgcacca aaaaccgacg aagcccgctg cgtacgccac ttgcgcctgc
      901 atgatttgtt gatcgttgac atcgatcgtc aatgtaccgg tcagcatcga cacatgcaca
      961 tggttgagcg cttgatcggc gttcatggcc ggtgatgatt gggcatcagc caacgcttga
     1021 tatgatccac cgacaatgtg gtacagctga taacggccat cattgatcac gcggaacaga
     1081 taagcattgt taatatcctg cacgccaaat aaaattccca gttgcgaggt acccgtgccg
     1141 gtgaggatct gcgcatcgac attcagatca aaattattga aggccacatt tggattccat
     1201 gttgatacgc cgtaagcctt gcccggatac acggcttcga tcacgaactt gccattctcg
     1261 atcgtgctgg tgccgttcga tgtatattgc tgcggcgccc aaccattgcg attgtcgtcg
     1321 aatgaatcga tcaacgcgac ttcatgcgcg gcgaaagttt ccggcgcgga gcatacgatg
     1381 acattatcga agcgcgcatc gacaccaccc cgattgaacg acgaagcgat caagccgatg
     1441 cgcttatccg aattgggatc gccgacatct tcgatcaact tcaacagcac atcattcgca
     1501 tacaacgcga tctgttggcc gcgttgaatg acgcgcagat aattgattgc ttggccgggc
     1561 ttcaaggcgg gcgcgaatgt agaatcttga atcggcgtcg atccatttga agtgcggcga
     1621 gtgatcatga agttgcgatt cgcatcgacg acgaaggcga tgtaatcatc ctgcccgcgc
     1681 gccgcaacga cgagaccgta agtgccgatc gcatctgtgt tgaactgcgc atcaacatcg
     1741 acttgataat ctttgaggtt cggtccaatt aattgggatt gaaagaaatt ggccgacttg
     1801 atcgacaacc gatattcgcc atcggccacg ccccacgtcg cgccttcttg ttcgccactc
     1861 ggaaaattta caggcgcatc aaagttttct gaatagatcg gcgtacaagc cgctacgttg
     1921 ttcgtgactg atgatgttgt cgcttgcggc gtgacgattg aactcggcgg cgtcggcgtg
     1981 agaatctgaa tgttcgatcc gatcgtcggg acaacgagcg gccgatcggg tggcggctgc
     2041 accgccgata aaacgattgc gcctccaacg cccaccacga tcaagcccaa catgatcaac
     2101 gcgatcacga tcgagcgtga taaaccaatc gagcgtggat gagttttggt gagatgcact
     2161 tgctcggtcg gggccgcagt cgatcgcaat ggttcagtgc tcggcggcgg cgcagatcga
     2221 atcgcatcag ctaacgcttc agcaaagccg ccgcaggtcg gatagcgatt gtcggggctt
     2281 tttgataacg atttcgcaac cacatcgctg atcgattcgg cgatacgcgg attgatcatc
     2341 ttgggcggcg tcaacggttc agtgacatgt ttcaacacaa tccccagcgg cgtctcggcc
     2401 tcgaacggcg gacggccgac gatcatttca tacgccatca cgccgagcga atattcatca
     2461 ctgcggccat cgatcttgat accctgcccc tgctcaggcg acatgtattg cggcgtgcca
     2521 atgacactgc tgcccgttaa accgatcgag ccttccatcc atttggcgat gccgaagtcg
     2581 gcgaggaacg cgttgccctg ctgatcgatc aagacgttgc tgggtttcac atcgcgatgg
     2641 atcacgctgc gctcatgagc gtaatcgagt gccgaagcaa cttgttcgag ccaacgcgcc
     2701 acatcgcgca atgcaaccat gcccgccgcc atgcgcgatt gcaatgaacc gaagcccatg
     2761 tagcgcatgg caatgtagac gatgccatct tgttcgccaa gatcataaac cggcagaatg
     2821 tgcgcgtgct ccagcgaagc gatcatctgc gcttcctgtt tgaagcgcgc gaggaacgtg
     2881 gcattgctcg ccatcaacgg cgacaaaatt ttgaccgcca ccgtgcgatt catgctgggc
     2941 tgcgtggctt tatagactgt cgccatgccg ccctgcccga tcggttcgag gattttatac
     3001 gggccgagtt gtttgccgat gagaggatcg tctgacatgg gcgctagtat aaatgatcaa
     3061 tgaacaatga tcaatttgta atgaacaatt tcaaacttgc catgcacgta acgaattatt
     3121 cattttcaat tgtgcattga taactcatcg ggtataatca ttcgcgtcgg ggacgtggcg
     3181 gaatggcaga cgcgcatggc ttaggac
//
LOCUS       DASRTJ010000253         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_317365,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000253 DASRTJ010000000
VERSION     DASRTJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317365"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFF70_05280"
     CDS             <1..326
                     /locus_tag="VFF70_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY44147.1"
                     /translation="FYDTPSYTGFVFPVDKFYPAWSLDEEHSLVKAGQQTIEALWGET
                     RPTGKWSFSTNGTYWAGKANIPSIGFGPGEEEWAHMTNEHIRLADVVHAAEWYALFPS
                     LLKGK"
     gene            579..1535
                     /gene="pyrB"
                     /locus_tag="VFF70_05285"
     CDS             579..1535
                     /gene="pyrB"
                     /locus_tag="VFF70_05285"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZY44148.1"
                     /translation="MRSFNGRDILSLKDFERQEFFRVFEIAKQLEPIARNRHNSDILK
                     DKTLVTAFYQPSTRTRLAHEAAMHRLGGHVTGFADAKMTRAGDFYQESIKDTVKMLEF
                     YGDVIVMRHFQQGAPHEAARWASVPIINGGDGWGEHPTQILTDLYTVLNEKGRLDGLK
                     WLAVGDMRMRTMHSLGYALSQFDCPITFVAPPDMSLTPEFKEELKQFSVNFKEADHVE
                     QAISEADVILVEPVVQPDYTKSRDERAGKEVNLTPANYKITRELLSTKAKSDAILLHS
                     LPRMDEIPTDVDITRWSRYWQEAFNGVVMRMALLTLILGKME"
     gene            1578..1847
                     /locus_tag="VFF70_05290"
     CDS             1578..1847
                     /locus_tag="VFF70_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44149.1"
                     /translation="MNSYEALKKESAHLTPEERLNILRLELLRPIVTVQSVTKLLTEI
                     DCEGIKHVPDHARPEEFDRLIQWLSEAGDDLKGILDAMTLNVIEK"
     gene            2009..2905
                     /locus_tag="VFF70_05295"
     CDS             2009..2905
                     /locus_tag="VFF70_05295"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY44150.1"
                     /translation="MLAAQQTTRAAYFILAFGIFCIGFSAIFVKLADLPGSTSGFYRL
                     GIASIALLPIYLARRSKYPPMSKRVFSLAALGGLFFALDVWAWQSALTYTSAASATLL
                     GNTSSIIVALGAWLIFREKLRLPFWTGLFIAFIGVLLVLGADFLTPHASDAAANIRTE
                     GNLLALLGACFYAGYLLTTQHTRARLDTVAYLFLMSAIGAIILLGFTIAQGDPLWGFS
                     IKSWLALLGLALITHVIGWLAISYALGHIRASIVSVTLLGQPVLTALFSIPLLGEGLV
                     PLQIIGGLLALSGIYIVNRFGR"
     gene            complement(2939..>4966)
                     /locus_tag="VFF70_05300"
     CDS             complement(2939..>4966)
                     /locus_tag="VFF70_05300"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZY44151.1"
                     /translation="VLELLVKEGGAVKTGDVIARLRSDAQQTAVARAEAGVAVAKANL
                     AKYQEQLPQLIASAEADVRSAQAQVASASAQNNDQAALASAQAALALATANQQQAQRA
                     YDDILKANKLGATEEQARLMLENARRSTAAAQLRFDQLKSGSLLARSNSLALAAAQAQ
                     QQAAQVNLDELTAEANGKPNPTYQAAVHQAETALQLAQAQLADTVLRAPFAGSIAQLT
                     LKVGETVGAGSPIGVLADFSGWQIVTVDLTEVKVPLISIGQSATIILDALPDVNLKGE
                     VQSIGQLYQEKSGDVVYPLKVKLIDLDPALRWGMTAHVTFDITSSIATAPAKSVTGQT
                     VTTAEGRVLPAQSANLSFNVGGQVAEILITEGDMIKAGDVIARLKNDSLRATLAQAQA
                     GVELAQAAQTNYQMQLPKLISQAGAALKAAQAKQLSASASGDNQAALLDAESALAHAK
                     YMQQQLETALDQLYVYDRANGSRADDLRAQLQSAIDTTQAAQAQVDALKTGSFGNLAN
                     TAQIKAASAEVTAAQALLDQLNAEAGGKATDTYAAAIQVAQAALLSAQQTLAQTEIRA
                     PFAGTVVQINLNLGEQVAADSPAIVLADLSSWRVETKDLTEIKVPLVKVGQAVIVTLD
                     ALPDVELKGTVESISGVSQLNGGDVVYPVKIDLSDSDPRLRWGMTVVVKFQ"
BASE COUNT         1064 a         1393 c         1311 g         1198 t
ORIGIN      
        1 tgttttacga tacgcctagt tacaccggtt tcgttttccc ggtggataaa ttttacccgg
       61 cctggtcgct ggacgaggag cattcgctgg tcaaggcggg acaacaaacg atcgaggcgc
      121 tgtggggtga aacgcggcca accggtaagt ggagtttttc cactaacggc acgtattggg
      181 ccggcaaggc caacattcct tcgatcgggt ttgggccggg cgaggaagaa tgggcgcaca
      241 tgaccaacga gcatatccgt ttagccgatg tggtgcatgc cgccgaatgg tatgccctgt
      301 tcccgtcttt gctaaaagga aaatagatta gatcaataac gctgtaatcc gatcgggagc
      361 gatcgcctat cgcgccgggc aaatttctcg gcgcaattta ttttatgtct cgatcgatcg
      421 gtctctattt gaacaaacgc acataagcaa aatggcagta gggtacagca gagatcgaat
      481 gacattcgac tgaatgaaaa tggaacaatg gtagtgtatt cgagcgcaca tttggactac
      541 cgtataggca acggaaaaaa cttactgagg agtcgaccat gcgcagtttt aacggacgcg
      601 acattttgtc tcttaaagat ttcgagcggc aggaattctt ccgcgtattt gaaatcgcca
      661 agcagctgga gccgatcgca cgcaatcggc acaacagcga tatcctaaaa gataaaacct
      721 tagtgactgc tttctatcag cccagcacac gcacgcgctt agctcacgaa gcagcgatgc
      781 atcgactggg cggccacgtc accggtttcg ccgatgccaa gatgacccgc gcgggcgact
      841 tctaccagga atcgatcaaa gacactgtca agatgttaga gttctacggc gatgtgatcg
      901 tcatgcggca ctttcaacaa ggcgcgccgc acgaagccgc gcgctgggcc tccgtgccga
      961 tcatcaacgg cggcgacggc tggggtgaac accccaccca aatcctcacc gatctttata
     1021 ccgtgttgaa tgagaaaggc cgcctcgatg gcttgaagtg gctggccgtc ggcgatatgc
     1081 gcatgcgcac gatgcattca ctcggctatg cgctgtcaca attcgattgc ccgatcacgt
     1141 ttgtcgcgcc gccggatatg tcactgacgc ccgaattcaa agaagaactg aagcagttta
     1201 gcgtgaactt caaggaagcc gaccacgtcg agcaggcaat cagcgaggcg gatgtgatcc
     1261 tggtcgagcc cgttgtgcag cctgattaca ccaagtcgcg cgatgagcgc gcgggcaaag
     1321 aggtgaatct cactccagcc aactacaaga tcacgcgcga actcttatcg accaaagcca
     1381 agtccgatgc gatcctcctc cactcgctgc cgcgcatgga cgaaatcccg accgatgtgg
     1441 acatcacccg ctggtcgcgc tactggcagg aagccttcaa cggcgtggtc atgcgcatgg
     1501 ctttactaac attgatcttg ggcaagatgg agtgatcgcc aaactcgatc gagtggttgc
     1561 tccttaagga ggccatcatg aattcttacg aagcgctgaa aaaagaaagc gcacacctga
     1621 ctcccgaaga acgcttgaat attctgcggc tggaactact gagaccgatc gtcaccgtgc
     1681 agagtgtcac taaactgctg acggagatcg attgcgaagg aattaaacac gtgcccgatc
     1741 atgcgcggcc cgaagagttc gatcggctca ttcagtggct cagcgaagcg ggtgacgatc
     1801 tcaaaggcat tctcgatgcg atgacactca acgttatcga gaagtaaaca cccagtgtct
     1861 tgatctatcc tttcccaaaa gagtgtgctg aatcgatcgg atcttgatgc gatcggttta
     1921 gcacactctt cgttttagct tataatcgat gttaaccggg ataacatcga ggcttgcaat
     1981 taccacaccc tcttcaacag attcatcaat gctcgccgcg cagcagacca ctcgcgcggc
     2041 gtattttatt ctcgcgttcg gcatcttctg catcggcttc tcagcgatct ttgtgaagct
     2101 ggccgatctg cctggctcaa cttcgggctt ttatcgattg ggcattgcct cgatcgcgct
     2161 gctcccgatc tatctcgctc gccgatcgaa atatccgcca atgtcgaagc gcgtgttcag
     2221 cctggccgcg cttggcggtt tgttcttcgc actcgacgtc tgggcgtggc aaagcgcgtt
     2281 gacttacacg tcggcggcca gcgccacctt gcttggcaac acgtcatcga tcatcgtggc
     2341 acttggcgcg tggctaatct ttcgggaaaa actccgcctc ccattttgga ccgggctctt
     2401 catcgcgttc atcggtgtct tgctcgtcct cggtgctgat tttctcacgc cgcacgcgtc
     2461 cgatgctgcc gccaacattc gcactgaagg caacttgctc gcgctgctcg gcgcgtgttt
     2521 ctatgcgggc tatctgctta ccacgcagca cacacgcgcg cgattagata cggtcgcata
     2581 tctgttcctc atgtccgcga tcggcgcgat aattctgttg gggttcacga tcgcgcaagg
     2641 cgatccgtta tggggcttct cgatcaaatc gtggttggcg ttgctgggct tagccttgat
     2701 cacgcacgtg atcggttggc tggcgatcag ttacgccttg gggcacatcc gcgcctcgat
     2761 cgtttcggtg acgctgctcg gccaaccggt cttgacggcg ctcttttcga ttcccttatt
     2821 gggcgaagga cttgtgccgc tacaaataat tggaggcttg ctggcattga gtggaattta
     2881 tatcgtcaat cgatttgggc gataaatgga gagacgaccc aagggtcgtc tctattcact
     2941 attggaactt caccaccacc gtcatccccc aacgcaaacg cggatcgcta tcgctcagat
     3001 cgatcttaac cggatagacc acatcaccac cattgagttg cgacacgcca ctgatcgatt
     3061 ccacggtacc tttcaattca acgtccggca gcgcatcgag cgtgacaatc accgcttgcc
     3121 cgactttgac cagcggcact ttgatctcgg ttaagtcctt cgtttccact cgccaactcg
     3181 atagatcggc caacacaatt gcgggactat cagccgcaac ttgttcgcct aaattgagat
     3241 tgatctgaac caccgttccg gcaaagggcg cgcggatttc agtttgcgct aaagtttgct
     3301 gagcagacag taatgcagct tgtgccactt gaattgccgc agcataagta tcagtcgctt
     3361 taccgccggc ttcagcgttc aattgatcga gcagtgcctg agccgcagtc acttcagcag
     3421 atgccgcctt aatctgtgcg gtattcgcca gattaccgaa cgatccggtt ttcaatgcat
     3481 cgacttgcgc ctgcgccgcc tgcgtcgtat ctatcgcgct ttgcaattgc gcccgcagat
     3541 cgtccgcgcg tgaaccattc gctcgatcgt aaacgtacag ctgatcgagt gcagtctcta
     3601 attgttgttg catgtatttg gcgtgtgcta acgctgattc ggcgtccagc aacgccgctt
     3661 gattatcgcc gctagcggat gcgctgagtt gcttggcttg tgcggctttc agcgcagctc
     3721 cggcctgtga gatcaatttg ggcagttgca tttgataatt cgtctgcgcg gcttgagcca
     3781 attcgactcc ggcttgtgct tgagccagtg tggctcgcag cgaatcattt ttcagccgcg
     3841 cgatcacatc gccggctttg atcatatcgc cttctgtgat caatatctca gcgacttgtc
     3901 cgccgacatt gaaacttaga ttcgcggatt gtgcgggcag cacgcggcct tcggcggttg
     3961 tcacagtttg cccggtcaca gacttggccg gtgctgtagc gattgaagat gtgatgtcaa
     4021 atgtcacatg cgcggtcatg ccccagcgca aagccggatc aagatcgatc aatttaactt
     4081 tgaggggata gaccacatcg ccgctcttct cttgataaag ctgcccgatc gattggactt
     4141 cacctttcaa gttcacatcc ggcaacgcat ccaaaatgat cgttgcactc tggccgatcg
     4201 atatcagcgg cactttgact tcagtcagat caacggtcac gatctgccaa ccggaaaaat
     4261 cggccagcac accgatcggc gatccggccc cgacggtttc accgaccttc aatgtcagct
     4321 gtgcgatcga tccggcgaac ggcgcgcgca acactgtatc ggctagttgg gcttgagcta
     4381 attgcaacgc ggtttcagct tgatgcactg ccgcttgata cgtaggattc ggcttgccat
     4441 tcgcttccgc ggtgagttcg tctaagttta cctgcgccgc ttgctgctgc gcttgagcag
     4501 ccgctaacgc caagctattc gatcgggcta gcagtgagcc gctcttcaat tgatcgaacc
     4561 gcagctgcgc cgctgcggtc gatcgcctgg cattctccag catcaaacgc gcttgttctt
     4621 cagtcgcacc caacttattc gctttgagga tgtcatcgta agcgcgttgg gcttgttgtt
     4681 gattagcggt tgctaacgcc aacgccgctt gagcagatgc aagtgcggct tgatcattgt
     4741 tctgcgccga agcgctagcg acttgtgctt gcgccgatcg aacatcggct tcagctgagg
     4801 cgatcaactg cgggagttgt tcttgatatt tggcgagatt cgcttttgcc actgccacac
     4861 cggcttcggc gcgcgcgacg gcggtctgct gcgcatcgga tcgcaggcgc gcgatgacat
     4921 caccagtctt gaccgcaccg ccttctttta ccagcaattc caatac
//
LOCUS       DASRTJ010000254         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_317450,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000254 DASRTJ010000000
VERSION     DASRTJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317450"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFF70_05305"
     CDS             complement(<1..376)
                     /locus_tag="VFF70_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HZY44152.1"
                     /translation="MRLGIDIGGTFTDFVLLDDATGKITTYKCLTTPRDPSDAIEEGI
                     EALARSVPGYVDSLEEIIHGTTLVINAIIERKGARTGLITTQGFRDVLELGREIRYAP
                     YDVFAQLPEPLVPRERRLEVNER"
     gene            complement(463..981)
                     /locus_tag="VFF70_05310"
     CDS             complement(463..981)
                     /locus_tag="VFF70_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44153.1"
                     /translation="MDVYMIILRVIHILAGVLWAGWAFVSVAFIEPATRAVGPEGGKF
                     MQALSGKTKLIPAMLISPLLVIITGVLMYWKVSDGLSGSWLGSPAGLALTIGSLAGIL
                     AFVLGFVINRPAAEHMAALSREMQSAGKPPSPEQMAELRAQQQRLSKGGWYGAILLGI
                     AVIGMALAGALG"
     gene            complement(1060..1212)
                     /locus_tag="VFF70_05315"
     CDS             complement(1060..1212)
                     /locus_tag="VFF70_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44154.1"
                     /translation="MLSPIAEVNADRLSELSFPPRLGEHNATIYGEVLGYSDEKLSEL
                     RSRQVI"
     gene            complement(1212..>1615)
                     /locus_tag="VFF70_05320"
     CDS             complement(1212..>1615)
                     /locus_tag="VFF70_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZY44155.1"
                     /translation="ISPLMLTAMGEQVTRRGNTHQFFAPVSVYPTRDGFVYIAVGNDR
                     QWEALTKLSGFESLIDDQYQRNAGRIADVKQLNERISDCTRSVTTDDLIAALNQIGVP
                     IAKVNTLNDVLNEPLLRNDFAHAKTRALQLK"
BASE COUNT          342 a          468 c          440 g          365 t
ORIGIN      
        1 gccgttcgtt cacttcgagc cgacgttcgc gcgggacgag cggttcgggc agctgcgcga
       61 agacatcgta cggcgcgtaa cggatttcgc ggccgagttc gagcacatcg cgaaagccct
      121 gcgtcgtgat caagcccgtg cgcgcgccct tgcgctcgat gatggcattg atgaccagcg
      181 tcgtgccgtg gatgatctct tccaggctat cgacatagcc gggcacgctg cgcgccagcg
      241 cctcgatgcc ttcctcgatc gcatcggacg gatcgcgcgg cgtggtcagg catttgtagg
      301 ttgtaatctt gcctgtcgca tcatcgagca gcacaaaatc ggtgaaggtg ccgccgatgt
      361 caattcctaa acgcatgatg atctccgtga tgcgtgatac gtgaagcgtg atgcgtgatg
      421 agtagtgcag aaattttctc ttcgctcagg ccgagaacgt tcttagccga gcgccccggc
      481 taaggccatt ccaatcacag caatccccag cagtatggcg ccataccacc cgcctttgga
      541 aagtcgctgt tgttgggcgc gtagttccgc catttgttcg gggctgggcg gcttgcctgc
      601 tgattgcatc tcacgtgaca atgccgccat atgttcggca gccggacggt tgatcacaaa
      661 gcccaagaca aaagccaaaa tgccagccaa actgccgatc gtgagtgcca gccccgccgg
      721 ggaacctagc catgatccgc tcaagccgtc tgacactttc caatacatca gcacgccggt
      781 gatgatcacc agcagcggtg agattagcat cgccgggatg agtttggttt tcccgctcaa
      841 ggcttgcatg aacttgccac cctccgggcc gacagcgcgc gttgccggtt cgataaaggc
      901 caccgacaca aaagcccagc ctgcccagag cacaccagcc aagatgtgga tcacgcgcag
      961 gatgatcatg taaacgtcca ttgttttctc ctgaagcgtt tagtttggaa tcaaagaata
     1021 attcgattcg atctcaagtt gcacgagtgt ttccctcatt caaatcacct gccgactgcg
     1081 aagttcggat agcttctcgt cgctatagcc caacacttcg ccataaattg ttgcgttgtg
     1141 ctcgcccagt cgcggcggaa acgaaagttc agacaatcga tcggcattca cttcggcgat
     1201 tgggcttagc atcacttcag ttgaagtgcg cgggtcttcg cgtgcgcaaa gtcattgcgt
     1261 aatagcggtt cgttcaagac atcgttcagc gtgttcactt tcgcaatcgg cacgccgatt
     1321 tgattcagcg cggcgatcag atcatccgtc gtcacgctgc gtgtgcagtc gctgatgcgt
     1381 tcattcaact gcttcacatc ggcgatgcgg cccgcgttgc gttgatactg atcgtcgatt
     1441 aatgactcga agccggacaa cttcgtcaag gcctcccact gtcgatcatt gccgacggcg
     1501 atgtatacaa agccatcgcg cgttggatac accgagacgg gcgcaaagaa ttgatgcgtg
     1561 tttccacgcc gtgtcacttg ctcgcccata gcagtcagca tcaacgggct gatca
//
LOCUS       DASRTJ010000255         1749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_317509,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000255 DASRTJ010000000
VERSION     DASRTJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317509"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFF70_05325"
     CDS             <1..522
                     /locus_tag="VFF70_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZY44156.1"
                     /translation="AYDDIDVLAGQATVGLEIMQDLADVDTIVVPTGGGALVAGIAVV
                     AKSINPMVRIIGVNPAASPSAYLSKRDGQPYDPFEHEPTLAQGLAGGFGRVPFMIGMM
                     LIDEIVLVTEDEMKWAIAKIIGADQILLEASGAAGVAAVLSGKVKDTKKCAVVLSGGN
                     IDTETLKHVLNTV"
     gene            581..1624
                     /locus_tag="VFF70_05330"
     CDS             581..1624
                     /locus_tag="VFF70_05330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZY44157.1"
                     /translation="MSPLIFVFLIAFILSFALTPIAARIGLRLNMTDDPGGRRQHAGR
                     IARTGGIALYISFVAAILISQTLNLPRSDPNESTRLIGLLIGTTIMFLFGLIDDRVEL
                     KPGLQFVAQFTASIVAIGSLIIIERFSNPFTNQPTIIESWWTWIIVPLTVFWLMGMMN
                     TVNWLDGLDGLAAGIAAIFSFILLLAMLHETSTQQPQISLTPLPLALLGATLGFLPYN
                     FHPARVFMGSSGAMLLGFVLGSLGIIGGAKVATVLLVLAVPILDVAWLIVSRIRHHRS
                     PMQGGRDHLHFRLLDLGLSQRQIVIGYWIVSALFGALALLIEARIYKLLALGILGVVT
                     LGVMIALSKRSNS"
BASE COUNT          363 a          462 c          483 g          441 t
ORIGIN      
        1 gcctacgacg acatcgatgt gctagcggga caagccaccg tcggacttga gatcatgcaa
       61 gacttagccg atgtcgatac gatcgtcgtg ccgaccggcg gcggcgcgtt agtcgcgggc
      121 attgcggttg tcgccaagtc gatcaatccg atggtgcgga tcatcggcgt gaatccggcg
      181 gcatcgccct cggcttactt atcgaagcgc gatggtcagc cgtacgatcc attcgagcat
      241 gagccgacac tcgcgcaagg attggcgggc ggcttcggtc gcgtgccatt catgatcggc
      301 atgatgctaa tcgatgagat tgtattggtc actgaagatg aaatgaaatg ggcgattgca
      361 aaaataattg gtgcggatca aattttattg gaagcatccg gtgcggcagg cgtcgcggcg
      421 gtgttaagcg gcaaagttaa agatacgaag aaatgcgccg tggtgttatc gggcggaaat
      481 attgatacag aaacgttgaa acacgtattg aatactgtat aatccgtgtt gcgcattgcg
      541 tgtaccttat gaacgcacca cgctatacgc aatacttcaa atgtcacctc tcatcttcgt
      601 cttcctcatt gccttcatcc tctcctttgc attgacaccc atcgccgcac ggatcggcct
      661 gcgcctgaac atgaccgacg atcctggcgg acgacgacag catgcaggtc gcatcgctcg
      721 aacgggcggc atcgcgttgt acatcagttt cgtcgccgcc attctcatct cgcaaacact
      781 caacctgcct cgatccgatc cgaacgaatc gacacgcttg atcggcttgt tgatcggcac
      841 gacgatcatg ttcctcttcg gcctaatcga tgatcgggtt gaactcaagc cgggcttaca
      901 attcgtcgcg cagttcaccg cctcgatcgt cgcgatcggt tcattgatca tcatcgaacg
      961 tttcagcaat ccattcacca atcaaccgac gattatcgaa tcatggtgga cgtggatcat
     1021 tgttccgttg acggtctttt ggttgatggg catgatgaac accgtgaatt ggctggacgg
     1081 ccttgatgga ctggcggccg gcattgcggc gatattcagt ttcattttgc tgttggcgat
     1141 gctgcacgaa acatcgactc aacaaccaca gatcagtttg acgcccctgc ccctggcgct
     1201 gctcggcgca acgctaggtt ttctgccgta caactttcat cccgcgcgcg tcttcatggg
     1261 atcgagcggc gcgatgttgt taggatttgt tttgggaagt ttggggatca tcggcggtgc
     1321 gaaagtcgcc accgtgttat tggtgttagc agtgccgatc ttggatgtgg cgtggttgat
     1381 cgtttcccgc attaggcatc atcgatcgcc gatgcagggc ggacgcgatc atctgcattt
     1441 ccgattgctc gatctcggct tatcgcaacg gcagatcgtg atcggttact ggatcgtcag
     1501 cgcgttgttc ggcgcattgg ctttgttgat cgaagcgcgg atttacaaac tacttgcgtt
     1561 gggaatttta ggggtcgtta cattgggagt aatgatcgcg ctttcaaaac gatcaaactc
     1621 gtaggggcgc cgtttccgcg cccaggacga ggcgacctcg cccctacaat tacgatttct
     1681 tctttcgcct tgacggctca tcgcttggcg gcgtggaata atacacgcgc tgtccgcgca
     1741 ctccaccga
//
LOCUS       DASRTJ010000256         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_346848,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000256 DASRTJ010000000
VERSION     DASRTJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346848"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VFF70_05335"
     CDS             complement(<1..1396)
                     /locus_tag="VFF70_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44158.1"
                     /translation="MAQSFTPPSPVLVAPNISGDVFYGDYAFKVNTNNGTIIYNQARP
                     PVKLKATPLPAPAAPLNFFDRAAESAQLDLIINTHSRLTVVGPEGVGKSTLLRQVANS
                     DTAKKLPHGVVLADGADAEGTRLSINDVIQQLFDQLYDSEPYLKVTAVTARVHLAQTQ
                     PLVLIDNVRFPSPDSLRTLIDLFPQSPILLAMPYSPGGNLTRLVDLGPLPRTDAVQLF
                     AATLNQPTGKLNRATINAICATLNNVPLAITTVADAVRTNNLPVDDVLATLNQIKPAS
                     SDPIQAGLERAYGLVYSTLSPLERQVLAIAASLPAKSIDPEMIQRMVTGELATLPGGE
                     IKREGNEVNIGGVRVPIRDAANVPDKDDTQPTTINRVSAAIDKLKGMGLLRSNSPRLR
                     IAAGLRLIAQIDVDQDAIHNQLVFQLLNDVRLNKHLNWQYCADELGNILGAIEWAAGQ
                     QRWTDVILLGRAIDP"
BASE COUNT          386 a          457 c          528 g          440 t
ORIGIN      
        1 acggatcgat cgcgcggccc aataaaatca catccgtcca gcgctgctgt cccgccgccc
       61 actcgatcgc gccgaggata ttgccgagtt cgtcggcgca gtattgccag ttgagatgct
      121 tgttgaggcg cacgtcattc aagagttgaa aaacaagttg gttgtggatc gcatcttgat
      181 cgacatcgat ctgcgcgatc aatcggagtc ccgctgcgat acgcagacgc gggctgttgg
      241 aacgcaatag ccccatgcct ttgagtttat cgatcgctgc tgatactcgg ttgatcgttg
      301 taggctgcgt gtcatctttg tctggaacat tcgctgcgtc gcggatgggc acacgcacac
      361 cgccgatatt gacctcattg ccttcgcgtt tgatctctcc gccgggcagt gtggctaact
      421 cgccagtcac catgcgttga atcatttccg gatcgatcga tttggccggc aatgaagcgg
      481 caatggctaa gacttgccgc tctaagggag agagcgtgga atagaccaat ccatatgcgc
      541 gctccaggcc ggcctgaatg ggatcgctcg aagctggctt gatttgattc agcgtggcga
      601 gtacgtcatc cactggcagg ttgttagtgc gaacggcatc cgcgacagtt gtgatcgcga
      661 gcggcacatt attcagtgtg gcacagatcg cattgattgt tgctcgattg agcttgccag
      721 tcggttgatt caatgtcgcc gcgaataatt gaaccgcgtc cgtgcggggc aatgggccga
      781 gatcaaccaa gcgggttaaa ttaccgcccg gagaatacgg catcgccaac aagatcggcg
      841 attgggggaa gagatcgatc agggtgcgta gcgaatcggg cgaagggaat cgaacattat
      901 cgatgagcac caacggctga gtctgggcga ggtgaactcg cgcagtgacc gcggtcacct
      961 tgagataagg ctcggagtcg tacagctgat cgaacagctg ctggatgacg tcgttgatgc
     1021 ttaagcgcgt gccctccgca tcagcgccat cggccagtac gacaccgtgc ggtagttttt
     1081 tggcggtgtc actgttggcg acctgacgta gcaaagttga tttgccaact ccttcaggcc
     1141 cgacgacggt taggcggctg tgcgtgttga tgatcagatc gagctgcgca ctttcagcag
     1201 cgcgatcgaa aaaattgagt ggagcagccg gtgccggcag cggagtcgct ttgagcttga
     1261 ccggcggccg cgcctgattg taaatgatcg taccgttgtt ggtgttgact ttgaaggcgt
     1321 aatcgccata gaagacatcg ccgctgatgt tcggtgcgac gagcactggg cttgggggtg
     1381 taaaactttg ggccatttat tacctcccat cagttctgac agaggtcagg gagtggatga
     1441 agttaccaag cctcgtattt ataatttggc ttgaggttct tccagctgcg tgagatgtag
     1501 ttgcgcagct caatgtttca gattttcatc gagctacgaa tagctcaagg gttgatcgat
     1561 ttgattcatc gccgcgtccg cgcacgatgt tgtgcagcgt catgagtgct gcctaatcca
     1621 gcgctcgcga aaaagatgca agcattttga gcggcgcaag acctctaatt cttctgcaac
     1681 ttgctttgtg cttcagacaa ctttgcgcgg gcctgctcca catcaggata agcgaattct
     1741 tcttttagcg caactgcctc gctcaacgcc gatgctgctc cagcgaattt gcctaatgtt
     1801 aagagcatat c
//
LOCUS       DASRTJ010000257         5508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_317689,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000257 DASRTJ010000000
VERSION     DASRTJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317689"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFF70_05340"
     CDS             <1..263
                     /locus_tag="VFF70_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZY44159.1"
                     /translation="HSISAIMCFGLLAYLILPKISSHFWKAIVIITTLLIILYIGYSR
                     LFVGDHFLTDVLAGYGLGIAWSGLVYTVVELISKKKMKLKNA"
     gene            complement(852..1940)
                     /locus_tag="VFF70_05345"
     CDS             complement(852..1940)
                     /locus_tag="VFF70_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY44160.1"
                     /translation="MSLAINWLDELDEDELEVDRVDDAALTDETEPAEQVESVEDGLD
                     GLDVEHEPEDILDEGGFDVVMQYFQESARHQLLSVADERELARAVERGRIAEKKLART
                     RRSLSDTSRRQLHREVSQALTAREELIRSNARLVISIAKRYQNLGLPLMDLIQEGNIG
                     LLRAIDRFEPSRGLRLSTYATWWVRQRINRAVANQGRTIRLPAYLQDRLHQLYRVSQA
                     LEQSLGRVPNDTELAETLEVTPTDIRSLQTLAMPVVSVDDSLSEKDDESALEQIEDTQ
                     VTPVDDLVGQQLLCESIARALEELPARCALIVRMRYGLDGDQPHTLEAIARKLRLSRE
                     RVRQIERDAFTRLRLSADVRKQRQPLAV"
     gene            2415..4559
                     /locus_tag="VFF70_05350"
     CDS             2415..4559
                     /locus_tag="VFF70_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44161.1"
                     /translation="MSEQEQIRFQAQLLKTVTQAILAVDLTGRIIFWNRSAETLYGWT
                     KDEVQGRNIIEVLAAPLLMESARANLGQLRRGESWSGEFLVQRRDGTTFTANINAALI
                     QNDRSTLISLISISRDNADQRQIHELNRLLAEASALLSNVVDYDAQLSTLAQVAVPQI
                     ADWCAVHVLQADGSIVQVALAPAETTTRPEARDWLRHYLPNDDAGGLPAVLRSGEPKL
                     VAEVSLDELAAAAAIKSYMIVPLIVRPHIIGAITFVTAESGRRFDQNALALAENLASH
                     IAIYLDKARLYRESQRLNVELEQRVSEHTAELRAAVAQLKQSEATIQTLFRISKELNA
                     TLEVDTILDELAQAAIQIVNGESGFAGLRTADGMTVHKYFRQGMIIPFEYTWPLGRGI
                     PGWVLQHKVPYGTSDAAHDPVMQHDLSINTDIHSIICTPILSSFDEVLGYFDIRNKQD
                     AEGFTPADRDLLMALAPAASIAIQNALSYQQRLSAEAELQESYERLRALAANLELVRE
                     EERTQIARELHDQLGQALTAMKFDLAWLTDRLVAEDVTLAQKAKAVTAQMDTMIKTVR
                     RIATELRPGMLDDLGLGASIEWQARDFEKRTGITCDVNVSTPDLHLPRAESLALFRIF
                     QEALTNITRHAGAQHIEVQLAATPTTLTLQVHDDGRGIQADEIAGLHSLGLLGMRERA
                     QRLGGTFDIQGVPGDGTVVTVSIPLNSANDVS"
     gene            4574..5203
                     /locus_tag="VFF70_05355"
     CDS             4574..5203
                     /locus_tag="VFF70_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY44162.1"
                     /translation="MLRILLADDHAVVRQGVKQILAAAFAQATFGEAQNGRELLELVA
                     TERWDVVVLDLAMPDGNGLEVLKQIKHDQPQLPVLILSMYPEDQYAVRTIRAGAAGYL
                     NKESAPEELVQAIRKVLHGGEYISASVADELVLHARHEGDQPLHKHLSDREYQVLCLI
                     ASGKEVKEISTELTLSAKTISTYRARLLEKMNMRTNAELTHYAIQNALV"
     gene            complement(5362..5505)
                     /locus_tag="VFF70_05360"
     CDS             complement(5362..5505)
                     /locus_tag="VFF70_05360"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44163.1"
                     /translation="MRKLDQLITKETGVAAYVAEAPMACVAMGAGLALENYGILRRSV
                     SGL"
BASE COUNT         1250 a         1506 c         1487 g         1265 t
ORIGIN      
        1 gacacagtat cagcgccatc atgtgtttcg gcctgctcgc ttacttgatc ctgccgaaga
       61 tatcatcgca tttctggaag gcgatcgtta tcatcacgac gctactgatt atcctgtaca
      121 tcggctatag tcgcctgttc gtcggcgatc atttcctgac ggatgttcta gcgggttacg
      181 ggctgggcat cgcctggtcc gggttagtct ataccgtggt cgagctgatc tccaagaaaa
      241 agatgaagct taagaacgct taagaagaat caacctgctt aaaggtttaa gcaaattcca
      301 atcgcatatg atgggttagt tcgccggctt cgactacacg gcgggtgtat cttacgcgaa
      361 acgcgattta gtcaaaggac gcccgccgct ccgctctgga tgcttccgtg aattcgacag
      421 gaaattgctc tagattaaag ggcaactaac gcttgatcta agtcgcctcg agggggtact
      481 gttcgtggta gcttggctaa cgagcctatc ccataaactt tacaggactg atagaactgc
      541 tgttgcatca aacgggtcgc ctcagccttg agtaaagttt cctcatccat gaattggaat
      601 tcgtcactcc gctcgcattc tcggattgcc agccagatgt agtcaattct gattgcagtc
      661 atacctgaac tcgtcaagtg tttgacgtgt cactgaatca gatcaatcgg cggcagtgtg
      721 gcaaaggaaa tggctgatcg ataatgctac gtttggcgac ttcatgataa tcgcttcgca
      781 cctctgcctc tatcagcatg tggctgattc tattgccggc atcgaatgtc ggaaattgtg
      841 agttgtttgg actacacggc cagcggctgg cgttgcttgc gaacatctgc cgacagccgc
      901 aagcgggtaa aggcatcgcg ttcgatttgc cgtacacgtt cgcggctcaa cctcaatttc
      961 cgcgcgatag cttcgagcgt atgcggttga tcgccatcca atccataacg catccgcacg
     1021 atcagtgcac agcgggccgg cagttcctct aacgcccgcg ctatcgattc gcacagcaat
     1081 tgctgcccga cgaggtcatc aaccggcgtg acctgagtgt cttcgatctg ttccagagcc
     1141 gattcatcgt ctttttcgct cagtgaatcg tcgaccgaga cgacgggcat ggctaaggtc
     1201 tgcagtgatc gaatatcagt cggcgtaacc tccaacgtct cggcgagctc agtgtcgttt
     1261 ggcacacggc ccagtgactg ctcaagcgcc tgcgataccc gatacagctg gtgtaatcga
     1321 tcctgcaagt aagcgggcag tcgaatggtg cggccttgat tggccacagc ccgattgatt
     1381 cgttgccgta cccaccaggt cgcataggtc gaaagccgca acccgcgcga tggctcaaac
     1441 cgatcgatcg cacgcagcaa gccgatgttg ccttcttgaa tcaagtccat tagaggcagg
     1501 ccgagattct gataacgttt ggcaatactg atgactaggc gtgcattcga tcgtatcaac
     1561 tcttcacgcg cggtcagcgc ctgcgagact tcgcggtgca actgacgccg gcttgtatca
     1621 ctcaatgatc gccgagtgcg cgcaagtttc ttctcggcga tacggccacg ttccaccgcg
     1681 cgcgccaatt ccctttcgtc cgcgacagac agcaattgat gtcgggctga ttcttggaag
     1741 tactgcatca cgacatcgaa cccgccttcg tcgaggatgt cttccggctc atgctcaacg
     1801 tcaagcccat cgagcccatc ctcaacgctc tcaacttgct ccgctggttc ggtttcatcg
     1861 gttagtgcag catcatccac tcgatcgact tccagttcat cttcgtccaa ctcatccagc
     1921 cagtttatag ccaggctcat gtctgccaac tcctcaatgt ctctttttat aaaaaaattg
     1981 cggtactctg ttcggattct cggcttgccg accagttgta ttcaatagta accgcggtca
     2041 cagcgtagct cctccgtgtc ggacgtagcg ttggaccaga tcgatcggcg gctaaatcgg
     2101 tacagggttg cagtgttatc gccagttggt ggcaatcgaa aagtcaatcg atcgttgccg
     2161 tgttcaatgc ctttgatgat aacttcttca cacgccagtg actatcagtc tgttgccgaa
     2221 gaacttgtaa gcattgaaag aaaaaggctg tcggtatttg ccctacaccg ttctaatagc
     2281 ccgatttgct cgctccaagc tgactcattc tggtctataa tatagtgagt gacgtcgttt
     2341 caagcaccgt ctacaggcta aagcggcaag caaagacagg ctgggtcggt tctatgcaac
     2401 ccacatcagt atccgtgtca gaacaagagc aaattcgttt tcaagcgcag ctgctgaaaa
     2461 cggttacgca agcgatcctg gctgtcgatt taacgggtcg catcatcttc tggaaccgtt
     2521 ctgccgagac gctatatggt tggacaaaag acgaagtgca aggccgtaat atcatcgagg
     2581 tgttagcggc cccgctattg atggagtcag cccgcgcgaa cctggggcag ctgcgacgcg
     2641 gcgagagctg gtcgggtgaa tttctggtgc agcgtcggga cggcacaacc ttcacagcca
     2701 acatcaacgc cgcgctcatc cagaatgatc gcagcaccct gatcagcctg atcagcattt
     2761 cacgcgacaa tgcggatcag cggcagatcc acgaactcaa tcggctgctg gccgaagcca
     2821 gcgcgctgct cagcaatgtc gtggattacg atgcacagct cagcacgctg gcgcaggtag
     2881 ccgtgccgca gatcgccgat tggtgtgccg tgcacgtgct gcaggccgat ggttccattg
     2941 tgcaagtggc gcttgccccg gccgaaacca cgacccggcc ggaagcccgc gactggttgc
     3001 ggcattatct gcctaatgat gatgcgggcg gattgccggc ggtgttgcgc agcggcgaac
     3061 cgaaattggt ggccgaagtc agtctagatg aattggccgc cgcggccgcg atcaaatcgt
     3121 atatgatcgt gccgttgatc gttcgccctc atatcatcgg tgcgattacc ttcgtgacgg
     3181 cagagtccgg ccgccgcttt gatcaaaacg cccttgccct ggctgagaat ctcgctagcc
     3241 atatcgctat ctaccttgac aaagcacgac tatatcgtga gagccaacgc ctgaatgtcg
     3301 aactggaaca gcgggtcagc gaacacaccg ccgaactgcg cgcggcagtc gcacagctaa
     3361 aacaatcgga agccacgatc caaacattgt ttcgcatcag caaagagttg aatgcgacgc
     3421 tggaggtaga taccattctg gatgaactgg cgcaagctgc cattcaaatc gttaatggcg
     3481 aaagcggatt cgcggggctg cggacggccg acggcatgac cgtccacaag tattttcggc
     3541 aggggatgat cattccgttt gaatatactt ggccgcttgg gcgcggcatt ccgggttggg
     3601 tcttgcagca taaagtcccc tacggcacca gcgatgcggc ccacgatccg gtgatgcagc
     3661 atgatctctc aatcaacacc gatatccact ccattatctg tacgccgatc ctcagttcgt
     3721 tcgatgaggt gctgggctat tttgatattc gcaacaagca agatgccgag ggcttcacgc
     3781 cggccgatcg ggatctgctc atggcgcttg cgccagccgc atccatcgcc atccaaaacg
     3841 cgctatcgta tcagcagcgc ttatcggctg aagccgaact tcaggagtcg tatgaacgct
     3901 tacgcgcgct ggcggccaat ctggaattgg tgcgcgaaga ggaacggaca caaatcgcgc
     3961 gcgagctgca cgatcaattg gggcaagcgt tgacggctat gaaattcgat ctggcttggt
     4021 tgaccgatcg gcttgtcgct gaggacgtta ccctcgccca aaaggctaaa gccgtcacgg
     4081 cgcagatgga cacgatgatc aaaaccgtgc ggcgcatcgc gaccgaattg cggccgggga
     4141 tgttggacga cctcgggttg ggcgcctcga tcgaatggca ggcccgcgac ttcgagaaac
     4201 gcaccggcat cacgtgtgat gtgaatgtgt ccacgcccga cttgcatctg cctcgcgccg
     4261 agtctctggc attgttccgc atctttcagg aggcgttgac gaacattaca cgccacgccg
     4321 gcgcgcaaca tatcgaggtt cagctggccg ccacgcctac gacattgacg cttcaggttc
     4381 acgatgatgg gcgtggtatt caagcggatg aaatcgccgg cctgcactcg ctgggcctat
     4441 tgggaatgcg ggagcgtgcg cagcgactgg gcggcacctt cgatattcaa ggtgtgcccg
     4501 gcgacgggac cgttgtcacg gtgtccattc cccttaattc ggcgaatgat gtgagttaaa
     4561 ttggaggaat gtgatgctta gaattctatt agccgatgat cacgccgttg tgcgccaggg
     4621 cgtcaagcaa attctggccg cggcgtttgc tcaggccact tttggcgaag cccaaaatgg
     4681 acgcgaactt ctggagctag tcgccacaga aagatgggat gtggttgtgt tggatttagc
     4741 catgcccgat ggcaatggtc tggaggtgtt gaaacagatc aagcatgacc agccgcagct
     4801 gccggtgttg attctcagta tgtatccaga agatcagtat gcggtgcgaa ccattcgggc
     4861 gggtgcggcc ggttatttga ataaagagag tgcgccggaa gaactggtgc aggctatccg
     4921 caaagtcttg cacggtgggg aatatatcag cgcgtcggta gctgacgagc tggtgctgca
     4981 tgcccggcat gagggtgatc aaccgctgca taaacacctg tcggatcgcg agtatcaggt
     5041 gctgtgtctg atcgcttctg gcaaggaagt caaggaaata tctaccgaat taacgctcag
     5101 tgccaaaacg ataagcacct accgcgcgcg gctgctggaa aaaatgaaca tgaggacgaa
     5161 tgccgaactc acccattatg cgattcagaa tgccctagtt taacgcgcat tgcctggcaa
     5221 tataccttac acagaataat acgctcatca aaaaatgaaa catgcgtcat ttgcatttcc
     5281 agcataaaaa agagcgaacc gatcagttcg ctctaatcca aatattttac gggtgcgatc
     5341 acctttcccc cataattatt actacaaccc gctgacactt ctccgcagga tgccatagtt
     5401 ctccaacgcg agacccgcgc ccatcgccac gcaagccatc ggcgcttcgg ccacgtaggc
     5461 tgccacgccc gtttctttgg tgatcaactg atcgagtttg cgcaataa
//
LOCUS       DASRTJ010000258         1608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_53050,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000258 DASRTJ010000000
VERSION     DASRTJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53050"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>1608
                     /locus_tag="VFF70_05365"
     CDS             62..>1608
                     /locus_tag="VFF70_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZY44164.1"
                     /translation="MAHQDQIIVRGARTHNLKNISVDIPRDKMVVITGLSGSGKSSLA
                     FDTIFAEGQRRYVESLSAYARQFLGQMEKPDVDQIEGLSPAVSIDQRGASSNPRSTVG
                     TVTEIYDYLRLLYARVGIPHCPVCGREVQMQTSDQIVDAVLALPEGTRLQILAPLIKD
                     RKGHHQPVFDDVRKAGYVRVRVDRDLHDVNDHFELDRYKNHTIEAVVDRLVMKADMDR
                     ARLSDSIETALKLGDGAVIVNEIAGKDSIDHMYSERFACAYDGTSLPEIEPRTFSFNT
                     PHGACETCQGLGVKFEIDPDLIMPNPNLSLAQGAINVMEWRSDETGEDMYYNNLLREA
                     AKYYKIDWNTPVGQISQAKKDILLYGSGDEKLKINFKHRTGGVRIYETTFEGILPNLR
                     RRYAESTSDYIRTKIEEYMAQAPCPTCGGKRLKPAALAVTVSGKNISEVTSQPVVEAL
                     DWVKALRSNSARSGDGGNGSKPLPLTPRQATIAKQILKEINARLQFMVDVGLDYLTLE
                     RNTGSLSG"
BASE COUNT          403 a          450 c          431 g          324 t
ORIGIN      
        1 taattaacct ttgcattccg cgagcaaatc taaaatcaga aatctaaaat ctaaaattcc
       61 catggcccat caagatcaaa tcatcgtgcg cggcgcgcgc acccacaacc tcaaaaacat
      121 cagcgtcgat attccgcgcg acaaaatggt ggtcatcacc ggcctgagcg gcagcggcaa
      181 atcctcgctg gccttcgaca cgatcttcgc cgaagggcag cgccgctacg tcgaatcgct
      241 cagcgcgtat gcccgccaat tcctcggcca gatggaaaag ccggatgtcg atcagatcga
      301 agggctgagt ccggcggtgt cgatcgatca acgcggggcc agcagcaatc ctcgatcgac
      361 ggtgggtacc gtcacggaga tttacgatta cctgcgtttg ctgtatgcgc gcgtcggcat
      421 tccgcactgt ccagtctgcg gccgcgaagt gcagatgcag acttcggatc aaattgtcga
      481 tgcggtcttg gcgctgcccg aaggcacacg cttgcaaatc ctcgcgccgt tgatcaaaga
      541 tcgcaaggga catcatcaac cggtttttga tgacgtacgc aaagccggct atgtgcgcgt
      601 gcgcgtcgat agggatctgc acgatgtcaa cgatcacttc gaactcgatc gatataagaa
      661 tcacacgatc gaggccgtag tcgatcggtt ggtgatgaaa gccgatatgg atcgcgcgcg
      721 cttgagcgat tcgatcgaaa ccgcgttgaa gttgggcgat ggcgcggtga tcgtcaatga
      781 gatcgccggc aaagattcga tcgatcatat gtactccgag cggtttgcct gcgcgtatga
      841 tggaacttca ttgccggaga tcgagccgcg aaccttctca ttcaacacgc cgcacggtgc
      901 atgcgaaacc tgtcagggac tcggtgtgaa attcgagatc gatcccgacc tcatcatgcc
      961 gaatccgaat ctgtcattgg cgcagggcgc gatcaatgtg atggagtggc gatcggacga
     1021 gacgggcgaa gacatgtatt acaacaatct attgcgcgaa gccgccaagt attacaagat
     1081 cgattggaac acgcctgtcg gccagatttc acaagccaag aaagatatcc tgctgtatgg
     1141 cagcggcgac gaaaaactca agatcaactt caagcatcgc acaggcggcg tccgcatcta
     1201 cgagacaacc ttcgaaggca tccttccgaa tttgcgccgc cgctatgccg aatcgaccag
     1261 cgattacatc cgcacaaaaa tcgaagaata catggcgcaa gcgccctgcc ccacgtgtgg
     1321 cggcaaacgc ttgaagcctg cggccttagc tgtaaccgtg agtggaaaaa atatttccga
     1381 agttacctcg cagcctgtcg tcgaagcgct ggattgggtg aaggcattaa gaagtaacag
     1441 tgctcgctcc ggcgatggtg gtaatggcag caagcctctg ccactcacac cacgccaagc
     1501 caccatcgcc aaacaaattc tcaaagagat caacgcccgc ctgcaattca tggtcgatgt
     1561 cggcttggac tatctcactc tggaacgcaa caccggctcg ttgtccgg
//
LOCUS       DASRTJ010000259         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_242076,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000259 DASRTJ010000000
VERSION     DASRTJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242076"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..1367)
                     /gene="plsX"
                     /locus_tag="VFF70_05370"
     CDS             complement(390..1367)
                     /gene="plsX"
                     /locus_tag="VFF70_05370"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZY44165.1"
                     /translation="MPPIRIAIDASGGDYAPSEIVAGAIAGARQHSIELVLVGRSEQI
                     DEQLRQHDTQDLAIEIVDAPETIEMQEDPALAVRKKTNASIVVGCELVRDQRADAIIS
                     MGHTGAGMIAGLFTFGRLPGIDRPAAIVPYLGFKDTFLIDAGANTEVQPANLLQFAIM
                     GSLYVERVMGIPNPKIGLLSNGGEPNKGNAIGKQAYLLLAASKLNFVGNVEGHGAVNH
                     EANLLVADGFVGNIALKLGEGIVDEVLNQVEHEAAPIDDKLRGVLAKLREQHDYARIG
                     AAPILGINGLLFIGHGRSKAPAVVSAIGLAVKSVESKLLEAIVEGLKGS"
BASE COUNT          339 a          439 c          387 g          380 t
ORIGIN      
        1 atcagcacat cgttgggacc gaccggtgga atttctttgg tgatcagcgt caagcctttg
       61 acgcgttccg gtttgacgag ggctttcatg tggtggttgt tcatgagggt tccttacgca
      121 agcggatttt ttcgtacacg aattgcacga ataacacgaa tgatctgtca ttccgagcga
      181 agcgaggaat ctctatgaca ataaacagca atcgccgatg gggcagagat tcttcatcgc
      241 gcgtagcgcg attcagaatg acatgtgttt ggagtatatc actgcgctta aaaatgcgtc
      301 aatgcaaagg ttcaggtttt tggggtgagg tttgtccaat caatatggca gtgcgaatga
      361 aaacttgcat cttacaacct gcaacttcgt taactcccct tcaacccctc gacgatcgcc
      421 tccagcaact tactctcaac cgacttcaca gccaagccga tggcgctgac caccgcgggc
      481 gctttcgatc gaccgtggcc gatgaacaac aagccgttga tgccgaggat cggcgcggcg
      541 ccgatgcgcg cgtaatcatg ctgctcgcgc aatttcgcca gcacgccgcg cagtttgtcg
      601 tcgatcggtg cggcttcatg ctccacttga ttcaacactt cgtcgacgat gccttcgccc
      661 agtttcaaag cgatgttgcc gacaaaacca tcggccacca gtaaattggc ttcgtgattc
      721 actgcgccgt gcccttccac attaccgacg aagtttaact ttgatgcggc caacaacaga
      781 taggcttgct tgccgatggc attgcctttg tttggttcgc cgccgttgga taacaaacca
      841 atctttgggt tcggaatacc catgacgcgt tccacgtata ggctgcccat gatcgcaaat
      901 tgcagcaagt tcgcaggctg cacttccgta ttcgcgcccg catcgatcaa gaacgtgtct
      961 ttgaagccaa gatacggcac aatcgccgcc ggtcgatcga tgccgggcaa acggccaaac
     1021 gtaaacagtc cggcgatcat gcccgcgccc gtgtggccca tcgaaatgat cgcatcggcg
     1081 cgctgatcgc gcaccaactc gcaaccgacg acgatcgatg cgttcgtttt cttgcgcacc
     1141 gccagcgctg ggtcttcttg catctcgatc gtttccggcg cgtcgacgat ctcgatcgcc
     1201 aaatcttgtg tatcgtgttg gcgcagctgc tcatcgatct gttccgatcg gccgaccagc
     1261 accaactcga tcgaatgttg gcgcgcgccg gcgatcgcac ccgcgacaat ttcactcggc
     1321 gcgtaatcgc cgccggaagc atcaatcgca attcgaatag ggggcataag aagtccgttt
     1381 tcgggacgca gattaacgct gacaccagca ctgctccgca ttgcggtgca agtgtctcgc
     1441 tgattaattt ttatctgcgt taatcctaat aatcagcgtc caattttctt tcagtctagc
     1501 atcgcgttga tcattcgtca aactttgttc cggatgctat aatcc
//
LOCUS       DASRTJ010000260         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_242095,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000260 DASRTJ010000000
VERSION     DASRTJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242095"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..953
                     /locus_tag="VFF70_05375"
     CDS             72..953
                     /locus_tag="VFF70_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44166.1"
                     /translation="MRLLCPNCRESIDAETLTCTNQHRFTRDANGVLILLSTAFDRVL
                     SAFLSDLHLARAADRKRILDPTVYRYLPDIPATIGDAQFQMEWRLRRDDWQVIDRLLR
                     GRSGLRVLDIGAWNGWLSHRLAQLGHAVIGIDYFVDEYDGLGARRFYSIDWQAVQIDL
                     IDLNVIDEAFDVVILNRGLQFFPDPLAYIVTVQQKVARGGCLIVTGLPFFRDTRSKVQ
                     QVAKARRAFQDCYGRDLFLRPTKGYLDFSDRQQLQSIGLELKRYPQLWRADLKAWLKP
                     TAPTHRYGLWRRGAASP"
     gene            950..>1705
                     /locus_tag="VFF70_05380"
     CDS             950..>1705
                     /locus_tag="VFF70_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZY44167.1"
                     /translation="MKAIVALFALAVMVRALMLMLIPFDGLYGQDAFAYYDYSIVLRS
                     ALIHGQPLPAFFWPIGFPLHLVFAYSVFGVHPLTAQWVSIIAGALIAPLTYLVARECL
                     LDLDQARAQRAGLVAGSIMIVTGQLMISSLSIMSDVVALAWAAASAWLLLRYHRQLRS
                     IDLTLTVLSLSIAIMSRWAYALLALPWTLSVLSRWHSQWPVIKLRRAIELTSLTILIV
                     GSVLLVPQILGVTRLGTFEVHGWNPINAVRTEVT"
BASE COUNT          311 a          421 c          499 g          474 t
ORIGIN      
        1 gggtgcggcc agcgcgcagg tgttgatgct gctggcctat gtctggcaaa gccgtgagtt
       61 gcgggaggag tatgcgctta ctatgcccga attgtcgtga atcgattgat gccgagacgt
      121 tgacctgcac gaaccagcat cggtttacgc gtgatgccaa cggcgtgctg attttgttga
      181 gcactgcgtt cgatcgtgta ctatccgctt ttctgtccga cctgcattta gcccgcgcag
      241 ccgaccgcaa acgcattctc gatccgactg tttatcgata cttaccggat attcctgcga
      301 ccattggcga tgcacagttt caaatggaat ggcgcttgcg acgcgatgac tggcaagtga
      361 tcgatcggct gttgcgaggc cgatcgggtt tgcgtgtgct cgacatcggt gcgtggaacg
      421 gctggctcag tcatcggtta gcgcaattgg gacatgctgt gatcggtatc gattactttg
      481 tggatgagta tgacgggctg ggcgcgcggc gtttttattc gatcgactgg caagccgtgc
      541 agatagattt gatcgatctg aatgtgatcg atgaagcatt tgatgtggtg atcttgaatc
      601 gcggtttgca attctttcct gatccgttag cttatattgt cactgttcag caaaaggtgg
      661 cgcgaggtgg atgtttgatc gtgacgggtt tgccgttttt tcgagatacg cgcagcaaag
      721 tgcaacaagt ggccaaggcg cgacgcgctt ttcaagattg ctatggacgc gatctgttct
      781 tgcgaccgac caaaggctat ctagattttt ctgatcggca gcagttgcaa tcgatcggtc
      841 tggaactgaa acgttatccc cagttatggc gcgcggattt gaaagcatgg ctcaagccga
      901 ctgcgccgac gcatcgctat ggcctttggc ggcgcggagc agcaagtcca tgaaagcaat
      961 cgtcgcgctg ttcgcgctgg ccgtgatggt gcgtgcactc atgctcatgc tgattccatt
     1021 cgatggctta tatggacagg atgcgtttgc gtattacgat tattcgatcg tgctgcgatc
     1081 ggccttgatc cacggtcaac ctcttccggc cttcttctgg ccgatcggtt ttccgctgca
     1141 cctcgtgttt gcgtactctg tatttggcgt tcatccgctc actgcacagt gggtcagtat
     1201 cattgctgga gcattgatcg cgccgctgac ctatctcgtc gcgcgtgaat gtttactcga
     1261 tcttgatcaa gcgcgcgctc aacgtgccgg tctcgttgct ggctcgatca tgattgtgac
     1321 tggacagttg atgatctcca gcctatcgat catgtccgat gtggttgctt tggcgtgggc
     1381 ggctgcttcg gcctggctac tcttgcgcta ccatcgtcag ctaagatcga tcgatctgac
     1441 cttgactgtt ctcagcttat cgatcgcgat tatgagtcgc tgggcatacg ccttattagc
     1501 gctgccgtgg acgctgagtg tgttgagccg ttggcatagt cagtggccgg tgatcaagtt
     1561 gcgccgggcg atcgagttga cgtcgctcac aattttaatt gtcggatcag tcttgctggt
     1621 gccgcagata ctgggtgtga cccggctcgg cacctttgaa gttcatggct ggaatccaat
     1681 caatgccgtc cgtactgaag tgacc
//
LOCUS       DASRTJ010000261         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_15131,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000261 DASRTJ010000000
VERSION     DASRTJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15131"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2406)
                     /locus_tag="VFF70_05385"
     CDS             complement(4..2406)
                     /locus_tag="VFF70_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HZY44168.1"
                     /translation="MNFLHRLNAFTHKHLTALLGLFIFIAAFGSYVATLAPAQVPGDP
                     SEYTFIPWILGIAHPPGYGFYTLLASVWQHIIPIGSVAYRSNLLAACAGASSATLVYL
                     IVQRLTMGRSIGSPISKTEAHLPAVFAGLSFAAAIDVWQHSLHANAHIITLLLATLSV
                     FLLIEWWSTDRDRWLYLFALIVGFSPAQHPLLIFGFPAYLIFIVLVKPRLFLRPNKIL
                     ALSSCVLIGLSIFLYYPLRSPTTPFGVSDIHSWDTFLHFVSAEGLRVNLFHFGLADQL
                     TRFSVFINLLQLQFSSISILFAIPGIIWLARRSIKPFVLCFTFLVITYAFIINTVQDV
                     MAYLLLPFMMLAVFIGIGVWVVALAFVRSATLRRLMPIPIAFALLLIPINTFATNSPR
                     VSLKNYTVGSEWVNAVFDRFAGKGEYSILLAPWEAMTPLWVAQYVESRTLDPKDVTPV
                     YVTTATANPWLDSVFANFDKGPVYLADYRRPVVEGKLFRLRPEGTWPLWRVVAPGDTS
                     VPHMDHPLNISAGGIELLGYSIDRTTLEAGQTAHLTLAMRSSITPPHIMMPYALVSDK
                     RHAWTTDSRLLTPDWLPNEVIVDRYDITLPFNTPAAEVSLKLGIADLAAGHDLSFVDG
                     SSAIDLGSLQVITPIVPHIESAEDALADFNAQIALIDASAIGNGRSIGIKDKTELTIQ
                     PGGSIQVWLRWIAEQQIDESYTVFVHLINDANQLFAQQDYTPMGGAFPTQLWIPRWIE
                     GQMINDPYTIDLPSDLPPGEYSIEAGMYGMTSGQRVTIIGHDGSLAGDRVILFKVKVA
                     PP"
BASE COUNT          643 a          701 c          714 g          533 t
ORIGIN      
        1 gggttagggt ggggctacct tcactttgaa taaaatcacc cgatcgcccg ccagactccc
       61 gtcatgcccg atgatcgtca cgcgctggcc actcgtcatg ccgtacatcc ccgcctcgat
      121 cgaatactcg cccggcggca gatcactcgg tagatcgatt gtatatggat cattgatcat
      181 ttgtccctcg atccatctcg gaatccacaa ctgcgtcggg aacgcgccac ccatcggcgt
      241 gtaatcttgc tgcgcgaaga gttgattggc gtcgttgatc aaatgcacaa acacggtata
      301 cgattcatcg atctgctgct ccgcgatcca tctcagccac acttggatcg atcctcccgg
      361 ctggatggtc aattcggttt tatctttaat cccgattgat ctcccgttcc cgatcgcgct
      421 ggcatcgatc aaagcgatct gcgcgttgaa gtcggccagc gcgtcctccg ccgactcgat
      481 gtgcggtacg atcggcgtga tcacttgcag cgatcccaga tcgatggctg aacttccatc
      541 cacgaaactc aaatcgtgcc ccgccgcgag atctgcgata ccgagtttga gcgaaacctc
      601 agcggctggt gtattaaacg gcagtgtaat atcatagcga tcgacaatca cttcattcgg
      661 cagccaatcc ggcgtcagca aacgcgaatc ggttgtccac gcgtggcgct tatcgctgac
      721 cagtgcgtac ggcatcatga tgtgcggcgg cgtgatcgat gatcgcatcg ccagcgtcag
      781 atgcgcggtt tgtcccgcct caagtgttgt gcgatcgatc gaatagccaa gcagctcaat
      841 gccgcctgcc gaaatattta acggatgatc catgtgcggc acggacgtat cgccgggcgc
      901 gacgacgcgc cacaacggcc aagttccttc cggccttaag cgaaacaatt tcccttcgac
      961 cactggccgt cgataatctg ccagataaac cggcccttta tcaaaattgg caaatacact
     1021 atcgagccat ggatttgccg tggcggtcgt gacgtagacc ggcgtgacat ctttcggatc
     1081 gagcgtgcgg ctttcgacgt attgtgcgac ccataaaggt gtcattgctt cccacggcgc
     1141 gagcaggatc gaatattcac ctttgcctgc gaatcgatcg aacacggcgt tgacccactc
     1201 actgccgacg gtgtagttct tcagcgatac gcgcgggctg ttcgtcgcaa aggtattgat
     1261 cgggatcagc agcagcgcga acgcgatcgg gatcggcatc aagcggcgca gcgtggcgga
     1321 tcgaacgaag gcaagggcca cgacccagac accgattcct atgaataccg ccagcatcat
     1381 gaatggcaat aataaataag ccatcacatc ttgcacagtg ttgatgatga aggcgtaagt
     1441 gataaccaaa aaggtaaagc acaacacaaa gggtttgatc gatcgtcggg ccagccagat
     1501 gatgcccgga atagcgaaga ggatgctgat cgatgagaac tgtagttgca ataagttaat
     1561 gaacaccgaa aagcgggtaa gctgatcggc caagccgaaa tgaaacagat tgacgcgcag
     1621 accttccgcg ctgacgaaat gcaggaaggt gtcccacgaa tgaatgtcgc tcacgccgaa
     1681 cggcgtcgtc ggacttctta aggggtaata caggaagatg ctcaaaccga tcaacacaca
     1741 gctgctgagc gcgagtatct tgttcggtcg cagaaacaat cggggtttca ctaaaacgat
     1801 aaaaatcaaa taagcaggga atccaaaaat caacaatgga tgctgcgcgg gagaaaatcc
     1861 aacgatcaaa gcaaacagat acagccaacg atcgcgatcg gtgctccacc attcgatcaa
     1921 caagaacacg ctgagtgtcg ccagcagcag ggtgatgatg tgcgcgttgg cgtgcaggct
     1981 gtgctgccag acatcgatcg cagcggcgaa cgacaggccc gcaaacacgg cgggcaggtg
     2041 cgcttccgtt tttgaaatcg gtgagccgat cgatcggccc attgtcaaac gctgcacgat
     2101 caaataaaca agtgttgcac ttgatgcacc cgcacaggcg gcgagtagat tggatcgata
     2161 cgcaacgctg ccgatcggga tgatgtgctg ccacacggaa gcgagtaatg tatagaatcc
     2221 ataccccggc ggatgagcga tgccgagaat ccacggaatg aacgtgtact cgctcggatc
     2281 gccggggact tgcgcgggcg cgagcgttgc cacatacgat ccaaacgcgg cgatgaagat
     2341 gaataagccg agcagcgcgg tgagatgttt atgggtgaag gcgttgaggc gatgcagaaa
     2401 attcattcta ggattcaaca caataagagc ggacttcaca ggtcgtcacc ctgagcgcag
     2461 cgaagggtct ggcgcggttg tcagtcgatt gacatccgac ggcgccagat gcttcgcttc
     2521 gctcagcatg acgtgttggt gatttgctca tgcttcctca acagattcgc ggcagttgtt
     2581 cgccgtacgg c
//
LOCUS       DASRTJ010000262         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_53274,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000262 DASRTJ010000000
VERSION     DASRTJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53274"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFF70_05390"
     CDS             <1..1122
                     /locus_tag="VFF70_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44169.1"
                     /translation="AVTLFTKLEEFLGLPSETLTTLAIVFFIVLFQIARGSFQPLLDV
                     ILFRQDRGELDYIRNLSDRLLTTTDLQQFLENILTAVADLLRVQRVFIASLDADGQWR
                     LRAHIGPVELFGRALSEMHAAQTQPLENGPVIADGFWAWPLWARGDEGVIGSLAVAAR
                     SDSPDLTEDESDHLQTLIYQAAIALEDRRLQQEVFAAVERIMPEIDVIQRQRSVVRYI
                     DSPTQPPSDVMLMADPEFSNWVKDALNHYWGGPKLTASPLLKMQVVEQMQSSHAADAP
                     KALRAVLADAIDLLKPDGQRSLTAAEWVLYNILEMRFIQGRRVREIAMKLAMSESDLY
                     RKQKIAVESVAAALSKMEEQAAAQVSIDQPDPVVDRVAV"
     gene            1210..2151
                     /locus_tag="VFF70_05395"
     CDS             1210..2151
                     /locus_tag="VFF70_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZY44170.1"
                     /translation="MAESADESYWRSLLADREAAAVNGSAADPDRFSSARSGEAARPI
                     DLLWAAAEHACDCAEVIPVDVTGFNRGGLLIDWLGLHGFIPASHVAGLPTNADETIRK
                     EEFVKRVGQKLHAKIVELDRPRGRFVLSERLAYDDKSRLELLLSEMQPGQQRCGVVTT
                     VCDFGVFVDLGGIEGLAHISEISWGRINHPADVLCCGQRVDVYVMHIDPSQRRVALSL
                     KRLQPDPWTTVEQRYVIDRVVQGVITTVVDFGAFVKLEDGVEGLIHISELAEGNFLHP
                     NNVVHENDSVRVRILSIDPQHRRLGLSLRQANAPRNT"
     gene            2215..>2739
                     /locus_tag="VFF70_05400"
     CDS             2215..>2739
                     /locus_tag="VFF70_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44171.1"
                     /translation="MTKSSRSTKSARPTKRSEPKRRQLRFAFGARQREILGLILITLS
                     VLSMLALLRITSDSSLSDWWNRALRWLFGWGAYVIAIVIGLVGLGIFRIALGARLHIL
                     WRATIGLEIVFILILGAVHAFSLNTDLWNLFQAGEGGGVLGWALASVVAGFIGSRVAA
                     GIVFVILALIAVSVA"
BASE COUNT          557 a          696 c          807 g          679 t
ORIGIN      
        1 gcggtcacgt tgtttacgaa gctggaagaa tttctgggct tgcctagcga aacgcttaca
       61 accttggcga tcgtgttttt catcgtatta tttcagatcg cacgcggatc atttcaaccc
      121 ctcttggatg ttattctctt ccgacaagac cgcggtgaac tcgattacat ccgcaacctc
      181 agcgatcgat tgttgaccac aaccgacttg cagcaattcc tcgaaaatat tttgacggcc
      241 gtcgcggatt tattgcgtgt gcagcgagtc ttcatcgctt cactggatgc cgatgggcag
      301 tggcgtttgc gcgcgcatat cgggccggtt gaattatttg gccgcgcatt gtcggagatg
      361 cacgccgcgc agacgcagcc gctggaaaat ggcccggtca tcgccgacgg cttttgggcg
      421 tggccgctgt gggcgcgcgg cgatgaaggt gtgatcggct cgttggccgt tgcggctcga
      481 tcggattcac ccgatctcac cgaggatgaa tcggatcatt tgcaaacgct catttatcaa
      541 gccgccatcg ccttggagga tcgacgcttg cagcaagaag tcttcgcggc cgtcgagcgg
      601 atcatgccgg agatcgatgt catccagcgg cagcgcagcg tcgtgcgtta catcgattcg
      661 ccgacacagc cgcccagcga tgtgatgctc atggccgatc cggaattttc gaattgggtc
      721 aaggacgcac tcaatcatta ttggggcgga cctaaactca cggccagtcc attgctcaag
      781 atgcaagtcg ttgaacaaat gcaatcgtcg catgcagcgg atgcgcccaa ggctttgcgc
      841 gcagtgttgg cggacgccat cgatctgctt aagccggacg gtcaacgcag tttgaccgcc
      901 gccgaatggg tgctctacaa tattttggag atgcgcttta ttcaaggccg gcgcgtgcgc
      961 gaaattgcaa tgaaactcgc tatgagcgaa agcgatctct atcgcaaaca aaagatcgcg
     1021 gtcgagtccg tcgccgccgc gttatccaaa atggaagaac aggccgctgc tcaagtctca
     1081 attgatcagc ctgatccagt ggtcgatcgg gtcgccgtct gatttgcctg gatgggtcat
     1141 ttctctgtag aatagaccag tggatcgatc tctggtggtt gcgtggtagg aggaattgcg
     1201 atgatgccga tggctgaatc agctgatgaa agttactggc gatctttatt agcggatcgt
     1261 gaagcagccg cggttaatgg gtctgccgct gatcccgatc ggtttagctc tgcccgatcg
     1321 ggcgaagctg cccggccaat cgatttactg tgggcggcgg ccgagcatgc gtgcgactgc
     1381 gctgaagtta tcccggtcga tgtcactggc tttaatcgtg gcggcctatt gatcgattgg
     1441 ttaggcttgc atggtttcat ccccgcctca catgtggccg gcttgccaac taacgccgat
     1501 gaaacaatcc gcaaagagga atttgttaaa cgcgtaggac aaaaattgca cgcgaagatc
     1561 gtcgagctcg atcgaccgcg cggtcgcttc gtgctgtcgg agcgcttggc ctatgatgac
     1621 aaatcgcgct tggagctgtt gttgtctgag atgcaacccg gtcagcagcg ctgcggtgtc
     1681 gtcacaacag tgtgtgactt cggcgtattc gtcgatctag gcggcattga agggctggcg
     1741 cacattagcg agatttcatg gggtcgcatc aatcatccgg cggatgtgct gtgctgcggg
     1801 caacgcgtcg atgtgtatgt gatgcatatc gatcccagcc agaggcgagt ggcgttaagc
     1861 ttgaaacggt tgcaacccga tccgtggaca accgttgaac agcgttatgt gatcgatcgg
     1921 gttgtacagg gcgttatcac caccgtggtt gacttcggcg cgttcgtcaa gttggaagat
     1981 ggtgtggaag gcttgatcca catttccgaa ttggctgaag gcaacttctt gcaccccaat
     2041 aacgttgtgc atgaaaacga tagcgtgcgc gtgcgcattc tcagtatcga tccgcagcac
     2101 cggcgcttgg gcttgtcatt gcggcaagcc aatgcgccga ggaatacata atcgtcatcg
     2161 tagagcaagt gggcatctta cggatacatc aaaatatgta acggtacatt catcgtgact
     2221 aaatcatcac gcagcacaaa atcagccagg ccgacaaaac gcagcgagcc caagcggcgt
     2281 cagcttcgct ttgcattcgg tgcgcgccag cgcgaaattc ttggactgat attgatcacg
     2341 ctcagcgtgt tgtcgatgct agctttgtta cgcatcacca gcgacagcag cctgagtgat
     2401 tggtggaacc gtgcgctgcg ctggcttttt ggctggggtg catacgttat cgcgatcgtg
     2461 atcggtctag tgggtctcgg aatttttcgg atcgctcttg gcgctcgcct gcatattttg
     2521 tggcgtgcga cgatcgggct tgaaatcgtt ttcattctga ttctcggcgc agttcacgcg
     2581 tttagtctta ataccgatct gtggaatctg tttcaagctg gcgaaggcgg cggcgttctc
     2641 ggttgggcgt tggcgtctgt cgtcgccgga tttatcggta gtcgagttgc cgctggaatt
     2701 gtcttcgtga tcctggcgtt gatcgccgtc agtgtggcc
//
LOCUS       DASRTJ010000263         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_242173,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000263 DASRTJ010000000
VERSION     DASRTJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242173"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFF70_05405"
     CDS             complement(<1..519)
                     /locus_tag="VFF70_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44172.1"
                     /translation="MTTRTNLAELEALGLIQLSTVSEEAAYLFRHALIQDAAYASLIK
                     QDRRQLNLIVGETLERLYADRVEQIAPRLAAHFDSAGDDRRALKYFTLAGDQAAQQFA
                     NAEASTHYTRALDIAKHLPDLGNASSILMHLYLQRGQALHSIASYQGALDNYLDMQQF
                     AQHCGDRSLELHS"
     gene            complement(694..1221)
                     /locus_tag="VFF70_05410"
     CDS             complement(694..1221)
                     /locus_tag="VFF70_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44173.1"
                     /translation="MQTKSVLLLSVLAVIITACTPTPGGATAMLPNISTANIVEGKTI
                     SEFLAGLPGGSTLKTAFPQLFTVVEYVEQVTVCYQKIGAVAVRTYSDKVTPLSSGTVA
                     IIDKKALTDPANLIACLGNTLNPSAQAQPAISPCAKAYTLQKDNNEFFIAYIASTQEM
                     CAAFCSGLEGCAAAP"
     gene            complement(1349..>2475)
                     /locus_tag="VFF70_05415"
     CDS             complement(1349..>2475)
                     /locus_tag="VFF70_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44174.1"
                     /translation="MTELFGGDTRLAVKYGEQSLALTRQFDLRDQLPFTLNDLAFAYI
                     FLGQMDLGSAALTEARVLWRANHNLPMLSDSLANSALTEFYLGKFADSIQFAREGIRV
                     CESINNPWGLSHNGGMLGYTLLETGHFGEALRAWTTAVEFGEQSGLIATRIGIRADLG
                     WLHHLVGQNDLALEQAQRAHAYSETIMPIWKAWSASVLARLYLSLDRIAEAQVLIDRY
                     CPGVLLPHFRRLLRSGAVNVAIVRGELAFAQQQYQAAIDITDEVVEILRSIGAGPFLD
                     DAWLLKGRSLLQLDRLTEARSVLAQARSAAEAIGSHRIYWQVLAQSSEVEAILGNAAE
                     ADRFRQQAKAEIDFLASQIDLEEVRASFLNQDQVRSLLNE"
BASE COUNT          564 a          655 c          685 g          571 t
ORIGIN      
        1 gctatgcagt tccaaagatc gatcgccgca gtgttgggca aactgctgca tgtccaaata
       61 gttatcgagc gcgccttgat agctcgcaat ggaatgcaag gcttgcccgc gttgaagata
      121 aagatgcatc aagatcgacg aggcgtttcc caaatccggc aggtgttttg cgatgtcaag
      181 cgcacgcgta tagtgagtac tcgcctcggc attggcgaat tgctgcgcgg cttgatcacc
      241 ggccagcgtg aaatatttca gcgcgcgtcg atcatcaccc gcgctgtcaa agtgagcggc
      301 caatctcggc gcgatctgct ccactcgatc ggcatacaag cgctccagcg tttcgccgac
      361 aatcaaattc aattgccgtc gatcctgttt gatgagcgag gcataagcgg catcttgaat
      421 cagagcatgc cgaaaaagat aggccgcttc ctcggagacc gttgacagtt gaatcaagcc
      481 caacgcttca agttccgcta agtttgttcg ggtggtcatc tattctccat caatcttgcg
      541 gaggttggct tgaagaatca gcagcatcct gagcgcgtcc ttcgactgcg aattgaattt
      601 ggagaattca actctccgct ctggatgctt acgtttttct tgattgactg cgaatagcat
      661 ttcgccacaa aaaccttccc aaggttatcc tcattacggc gctgccgcac agccttccag
      721 cccggagcaa aaggccgcgc acatttcttg cgtcgacgca atgtaggcga tgaagaactc
      781 gttgttatct ttctgcaacg tgtaagcctt ggcacacggg ctgatggcgg gctgcgcctg
      841 agcacttggg ttgagggtgt tgccaaggca ggcgatcaaa ttggccggat cagttaacgc
      901 ttttttatca ataatggcga ccgtgccgga cgagagcggc gtgactttgt cgctgtacgt
      961 ccgcacggcg acggcgccga tcttttgata gcagaccgtc acttgctcca catattcgac
     1021 gacagtgaac aactgcggga aggcggtttt caaggtcgaa ccgcccggca agcccgccag
     1081 aaattccgag atggttttcc cttcgacgat gttggccgtg gagatgttgg gcagcatggc
     1141 cgttgcgccg cccggcgttg gcgtgcaggc ggtaatgatg acagctagta ctgatagtag
     1201 taatacggat tttgtttgca tgataactcc ttggtttcgg atgtcatgcc cgaatgctgt
     1261 tatcgggcat ccagttttac gattgacggg tcatgttgac agtgcggtgg attcccgctt
     1321 gaagcatgcg ggaatgaccc gccgacgatc attcgttgag cagcgatcga acttgatctt
     1381 ggttcaagaa agacgcgcgg acttcttcaa gatcaatttg actggccaga aaatcgatct
     1441 cggctttggc ttgttgtcta aatcgatcgg cttcggcggc gttgccgagt atggcttcaa
     1501 cttcactcga ttgggctaac acttgccagt aaattcgatg cgagccgatc gcttcggcgg
     1561 ccgatcgggc ttgagctaaa accgatcggg cttcagttaa ccgatcgagc tggagcagcg
     1621 atcgaccttt cagcagccag gcgtcatcca aaaacggacc ggccccgatc gatcgcaaaa
     1681 tttctacgac ctcatccgtg atatcgatcg ctgcttgata ttgctgttgg gcaaatgcga
     1741 gttcgccgcg cacgatagcg acattgactg cgcccgatcg caacaagcgc ctgaagtgcg
     1801 gcagcagaac gccgggacaa tagcgatcga tcaacacctg cgcttccgcg atgcgatcga
     1861 gcgatagata taagcgcgcc aatacgctgg ccgaccatgc tttccaaatc ggcatgatgg
     1921 tttcagaata agcgtgcgcg cgctgcgctt gttcaagcgc caaatcgttt tgaccgacca
     1981 agtgatgcag ccagccgaga tcggcgcgga tgccgatgcg cgtggcaatc aagccggatt
     2041 gttcaccgaa ttcaaccgcg gtcgtccagg cgcggagcgc ttcaccgaaa tgcccggtct
     2101 ctagcagcgt gtaacccagc atgccgccat tgtgcgacag tccccatggg ttgttaatcg
     2161 attcgcacac gcggatgcct tcacgcgcga attggatcga atcggcaaac ttgcccagat
     2221 aaaattcggt cagggccgaa ttggctaaac tatcagagag catgggcaag ttatgattcg
     2281 cgcgccataa aacacgggct tcagtcagcg cggcagatcc gagatccatt tgtccgagaa
     2341 aaatataggc gaaagccaaa tcgtttagcg tgaagggcag ctgatcgcgc agatcgaatt
     2401 gccgcgtgag ggctaatgat tgctcgccgt attttacggc caggcgggtg tccccgccaa
     2461 ataattcagt catca
//
LOCUS       DASRTJ010000264         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_318071,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000264 DASRTJ010000000
VERSION     DASRTJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318071"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFF70_05420"
     CDS             <1..311
                     /locus_tag="VFF70_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZY44175.1"
                     /translation="IIFLTLLSTWIFINSLINKPFDPFPFILLNLFLSMIASLQAPVI
                     MMSQNRSNANDRIRAELDYNVNLKAELEVAQLHEKLDAVREELLGAIGVILQRDARSS
                     "
     gene            complement(381..1304)
                     /locus_tag="VFF70_05425"
     CDS             complement(381..1304)
                     /locus_tag="VFF70_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44176.1"
                     /translation="MEYIVMLQRAWQILTRHKILWVFGLSAVLAGQDALFNLHGAQAV
                     QPLTEAIVNLPPTAGTLARSIVPLGSDLVLVGVMIIFGLGLLFIGMLASAAMIGLTQS
                     AERGDPVDFKTGLRAGFQHIDRLIIIRLIFNIPFVVLSLAGFTTLLRTIDPATVPLSY
                     GQSLNVLQDTGLLTIFLIISLITGIFIGAIGIGADRAVVIDGMGVIAALRQGWDTLRN
                     NLGRYILITGIFISTLLLFTLLLACPLAIVFSDRIAALTQTAPLNSDLALILLNEPAG
                     VSVLIIGGLLYAGVTAYSSIVWTIAYRRYAR"
     gene            complement(1333..2043)
                     /locus_tag="VFF70_05430"
     CDS             complement(1333..2043)
                     /locus_tag="VFF70_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44177.1"
                     /translation="MNLLVMLAVVLTACGQPSAPVVMTPIELPTVVLPPTRTPTPTLA
                     VEHAVRTVEPTAVPSIAATAALTIARTIVPPATPAASIVYTSTDAIFTLQFPANWAKQ
                     SGERQLLTSQLQHMAYVWFSAPGTPPQPAMIIFYNWPAVDVISNDTAWQQAFALASLA
                     IKNCAMNLGSNTSAPIDIAGEHAQFIGYTDACGEQGELIGLVHHGLNAGILIEAPQSL
                     WREWRPILRGIIGSLTLN"
     gene            complement(2052..>2653)
                     /locus_tag="VFF70_05435"
     CDS             complement(2052..>2653)
                     /locus_tag="VFF70_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44178.1"
                     /translation="KKFLPLLALRVLLALPTVIVGAIAAGSFLSIFSDLTTEPSQSTN
                     LFNFGNFGTLAGLGLMVFVLTLLFGAISISAERAVVLDDLSIFAAIARGWKYIWSKFE
                     DYFTIGLLFILVSIAFGILFVCALAPFLLASLGAALGSIQSGVNTFAFNFNLIGPAAI
                     AAVIIGLLFGTFFSVFEVSVWTLAYRVWHMTDTTASTSI"
BASE COUNT          651 a          744 c          737 g          521 t
ORIGIN      
        1 tcatcatctt tctcacgctg ctgtcaacct ggattttcat taactcgctg atcaataagc
       61 cgttcgatcc gttcccgttt attctgctca acttattctt gtcgatgata gcctcgctgc
      121 aagcgccggt gatcatgatg agccagaacc gctcgaatgc gaatgatcgt atccgcgccg
      181 aactggatta caacgtgaac ttgaaagcgg agttggaagt ggcgcagctg catgagaaac
      241 tggatgccgt gcgtgaagag ctcttggggg cgatcggcgt gatcttgcag cgcgacgccc
      301 gctcgagcta gacaaaaaag ggcggccaca accggtcgcc ctttttattt aacccgagat
      361 caggctgctt tccttatttc ttagcgcgca taacgtcgat aagcgatcgt ccacacgatc
      421 gatgagtagg ccgtaacgcc cgcatacaac agcccgccga tgatcaacac gctgacgcca
      481 gcgggttcgt tcaacaggat caaagccaga tcggaattaa gcggcgcggt ctgcgtgagc
      541 gcggcaattc gatcggagaa tacgatcgcg agcgggcagg ccaacagcag cgtaaacagc
      601 agcaacgtcg agataaaaat tccggtaatg agaatgtagc ggcccagatt gttgcgcagc
      661 gtatcccagc cctgccgcag cgcagcgatc acgcccatcc cgtcgatgac taccgctcga
      721 tcggcgccga tgccgatcgc cccaatgaag attcccgtga tcaaactaat gatcagaaag
      781 atggtcaaca gccctgtgtc ttgcaacaca ttcaacgatt gcccgtaact gagcgggacc
      841 gtcgccggat cgatcgtccg tagcaacgtg gtaaaaccgg ccaaggacaa cacgacaaac
      901 ggaatgttga agatcaagcg gatgatgatc aatcgatcga tgtgctgaaa cccggcgcgc
      961 agtccggttt taaaatcgac cggatcgccg cgctcggccg attgcgtcag cccgatcatc
     1021 gcggcgctgg ctaacatacc gatgaacagc agccccaaac cgaaaatgat catcacgccg
     1081 accaacacca agtcgctgcc gagcggcaca atcgatcggg ccagcgttcc ggcggtaggc
     1141 ggcagattga cgatcgcctc cgtgaggggc tgcacggcct gcgcgccatg caaattgaac
     1201 aacgcatcct gcccggccaa gaccgcgctg aggccgaaca cccacaaaat tttgtggcgc
     1261 gtgagaatct gccacgcgcg ctgcagcatc acaatatatt ccatttaagt ctccgcttga
     1321 gcaacccggt ttctagttta gcgtgagtga tccaatgatg ccgcgcagaa ttggccgcca
     1381 ctctctccat aacgactgcg gcgcctcgat caaaatacca gcatttaaac cgtgatgaac
     1441 cagcccgatc aattcgcctt gctcgccaca cgcatccgtg tagccgataa attgagcatg
     1501 ctccccggcg atatcgatgg gtgcgctggt attgctgccc aagttcatgg cgcaattttt
     1561 gatcgcgagc gacgccagtg cgaaggcctg ctgccaggcc gtatcattgc taatgacatc
     1621 caccgccggc caattgtaaa agatgatcat cgccggctgc ggcggtgtgc ccggtgcact
     1681 gaaccagacg taagccatat gctgcagttg actggtcagt aactgccgct cgccgctctg
     1741 cttggcccaa ttcgctggaa attgtaaggt gaagatcgca tcggtcgatg tataaacgat
     1801 cgaggctgcg ggtgttgcgg gcggaacgat cgtgcgcgcg atcgtcagcg cggctgttgc
     1861 tgcaatcgat gggacagcag ttggctcgac ggtacgcact gcgtgctcta ccgcaagtgt
     1921 cggtgtcggc gtcctcgtcg gtggtaacac cacggtggga agttcaatgg gtgtcatgac
     1981 caccggcgcg ctgggttggc cgcacgctgt caatactact gccagcatga ctagaaggtt
     2041 cacccgaggc atcagatgga agttgaagct gttgtgtcag tcatgtgcca gacgcgataa
     2101 gctaatgtcc aaacactcac ttcgaacacg ctgaagaaag tgccgaagag caaaccgatg
     2161 atcaccgcgg cgatggccgc tggcccgatc agattgaaat tgaatgcaaa agtattcacg
     2221 cccgattgga tgctgccgag tgcggcgcca aggctggcaa gcaagaacgg cgcgagtgcg
     2281 cacacgaaga gaatcccgaa ggcgatcgat actagaatga agagcaagcc gatcgtaaag
     2341 taatcttcga atttgctcca gatatatttc cagccgcgcg cgatggccgc gaagatcgat
     2401 aaatcgtcta agaccacggc gcgttctgcg ctaatgctaa tcgcgccaaa caaaagggtc
     2461 agcacaaaca ccatcaggcc taacccggcc agcgttccaa aattgccgaa gttaaacaag
     2521 ttggtagatt ggcttggttc ggtggtcagg tcggagaaga tcgacaagaa tgatccggcg
     2581 gcgatcgcac cgacaatgac cgtcggcagc gccagcagga cgcgcagggc cagcagcggc
     2641 aggaattttt tac
//
LOCUS       DASRTJ010000265         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_90576,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000265 DASRTJ010000000
VERSION     DASRTJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90576"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VFF70_05440"
     CDS             <1..178
                     /locus_tag="VFF70_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44179.1"
                     /translation="FYRAPNVSAEEQGTGLGLAIVKSIVDQHGGRVWVTSAPGKGSTF
                     SVALPIWAETTSNE"
     gene            315..1760
                     /locus_tag="VFF70_05445"
     CDS             315..1760
                     /locus_tag="VFF70_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZY44180.1"
                     /translation="MLDETRIQQIVQQVVAELRQSGSTPNRSDAARSASIGLAGVDGL
                     FPDVDSAVAAAKSAFTQLNALPLELRERMVSAMRSAAEEAAQVLAHEAWSETGMGRYE
                     DKIEKNLLNARKAAGTEDLKTTAWSGDHGLTIVEYAPYGVIGAITPSTNPTSTVICNA
                     IAMVAAGNAVVFGAHPMAKNASLHTMQVLNLAIQSVGGPKNLLTGFAAPTIEKAQQLM
                     AHKDVRLITVTGGAEVVKQAMKSGKRAVCAGPGNPPVVVDETADIDQAGRDVVCGGSF
                     DNNIVCTSEKETFVVESVGDALVKSMCAHGGLLINAWQLERLCKVIFDENRGPGKPGV
                     MNKKFIGKNASVLLKEIGIDAGPEVRQIVAVVDRDHPLIWSEQMMPIMPVCKVKNCDD
                     AIDLAVQAEHGFRHTAVMHSKNLDHLSRMARECNCSIFVKNGPCLSGLGYNGEGYCSF
                     TIASPTGEGVTSPRSFSRIRRCTLVDSFRIV"
     gene            1821..2345
                     /locus_tag="VFF70_05450"
     CDS             1821..2345
                     /locus_tag="VFF70_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44181.1"
                     /translation="MTQLGARRLTADIFADSHRISTQMSLRGRVLIDILNDPNTSYLD
                     LDIAYVSRIDHPGEILADYSLSIVRKDRLTFIVVATMGEMALKQNPALKVGMQLWPVF
                     VTVPGFEITGQIAAPANLDLRTLMAIGGERFMPVFDAKATLSMNQSVEFGGELVLINK
                     SAIEVLCVSNKLER"
     gene            2362..3444
                     /locus_tag="VFF70_05455"
     CDS             2362..3444
                     /locus_tag="VFF70_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44182.1"
                     /translation="MATILVIDDDLDLQQMLRLMLQRGGHKVITTGDGGDGLAKAKQL
                     HPELAIVDVMMPGMNGYQVVRKMRAEPETANTAILILTARAQTVDREAALAAHADDYL
                     AKPVAPNELLTKVNELLAQRKASQGPAQETIALMSLRGGVGVTSIAVNLALAWQLKNR
                     ATCLIDLKPGPGHIALHLRLNAKTTWIDWANSGDTPIDVLSNYLLPHDSGLSVLAAPI
                     VPADVPVPDRVAKLIGLLREKFARVVIDMPSNWDVLGASIATASDAIWLVIAPEVGSL
                     QSTVGAMRVLKTTKIPDERVQLIANQVMPKPGLAMQAIEKALGHGFKGSLPYDEAQSA
                     AIAQGLPLMTSQSSSPLAVAIRSLTV"
     gene            3452..4102
                     /locus_tag="VFF70_05460"
     CDS             3452..4102
                     /locus_tag="VFF70_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZY44183.1"
                     /translation="MVKRAARSKAASPETPRETALALIEFDSIATGLQAGDAMIKRAP
                     IPRIVAGTVQPGHYLVLISGTVADVEESLKAGREVGGSNVVDTVYLPQVHSAVVDAIA
                     GGRNPQPGTALGIIETSTVPAAIQAADAGVKGAQVALLEIRLADGLNGKGLTLFTGDV
                     ADVEVAIEIGVKSIDARMLTNKVIIPQIHPEMNANVIERTRFGSNVSEYWPLTNDE"
     gene            4233..4520
                     /locus_tag="VFF70_05465"
     CDS             4233..4520
                     /locus_tag="VFF70_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZY44184.1"
                     /translation="MQLAKVTGTLVATQKYDGLEGVKFLIVQPLGKHQKPEGDPIVAA
                     DGTAQAGPGMTVFVVASREAALALPVKFVPVDHAIVGIVDQVDIAKSHRAE"
BASE COUNT         1084 a         1310 c         1346 g          971 t
ORIGIN      
        1 tttctatcgg gcacccaacg tctccgccga agaacaaggc accggccttg ggctggcaat
       61 cgtcaaatcg atcgtcgatc agcatggtgg tcgcgtgtgg gtcaccagcg cgccgggcaa
      121 aggttccacc ttctcagtgg cgctgcccat ttgggcagag acgacgagta atgagtaaat
      181 catcacgcgt agcacgccac ccagccgtta accggggaat gagatgagca aaaatgtgaa
      241 ttctgcccta agcaatgttg aaaggtcgca gaattcatct tggagtcgat gaaagcgaat
      301 cagcgagaaa cgaaatgctc gatgagaccc gtatccaaca aattgtgcaa caagtcgtgg
      361 ccgagctgcg gcaatcggga tccactccaa accgatcaga tgcagcacgc tccgcgtcga
      421 tcggcttggc tggcgtcgac ggtttattcc ccgacgtcga ctcggccgtc gccgccgcta
      481 agtcggcctt cacgcagttg aatgcactgc cgcttgaatt gcgcgaacgc atggtcagcg
      541 caatgcgatc ggccgccgaa gaagccgccc aagtcttggc tcatgaagcg tggtccgaga
      601 caggcatggg acgctacgaa gacaagatcg aaaaaaactt gctcaatgcg cgcaaagcgg
      661 ccggcactga agatctcaag accacggcgt ggagcggtga tcacggtctg acgatcgtcg
      721 agtatgcgcc atatggtgtc attggcgcaa tcacacccag tacgaacccg acctcgacgg
      781 tgatctgcaa tgcgatcgcg atggtcgctg ccgggaacgc ggtcgtgttt ggtgcacatc
      841 ccatggcaaa aaacgcttca ttgcatacga tgcaagtgtt gaacctcgcg atccaatccg
      901 tgggcgggcc gaagaattta ttaacgggtt ttgcggctcc aacgattgaa aaagcgcagc
      961 aactgatggc gcacaaagac gttcgattga taaccgtgac gggcggcgcc gaagtcgtga
     1021 agcaagcgat gaagagcggc aagcgcgcgg tgtgcgccgg accgggcaat ccgccggttg
     1081 tcgtggatga aacggcggac atcgatcaag ccggccgcga tgtggtatgt ggcggctcgt
     1141 tcgataacaa tattgtctgc acgtccgaaa aggaaacatt tgtggtcgag tcggtcggcg
     1201 acgccctcgt taaatcgatg tgcgcgcacg gcggcttatt gatcaatgcg tggcagctcg
     1261 agcgcctatg caaagtaatc ttcgacgaga atcgcggacc gggtaagccg ggtgtgatga
     1321 acaagaagtt tattggcaag aatgcctccg tgctgttgaa agagatcggc atcgacgccg
     1381 ggccggaagt gcgtcagatc gtcgccgtgg tcgatcggga tcacccgcta atctggtcgg
     1441 agcaaatgat gccgatcatg ccggtctgca aagtaaaaaa ctgtgacgat gcgatcgatc
     1501 tggcggttca agccgaacac ggtttccggc acaccgccgt gatgcattcc aagaatctgg
     1561 atcatctcag ccgcatggca cgcgaatgca attgcagcat cttcgtcaaa aatggaccat
     1621 gtctgtccgg tctcggctac aacggcgaag gctattgttc gtttacgatc gccagcccca
     1681 ccggcgaagg tgtgacctcg ccgcgcagtt tcagtcgcat ccgacgctgt acgctggtgg
     1741 attcatttag aattgtttag tgcctcaccc taaccccgac accgcatgtc aaaggggatg
     1801 aggtgaggga ggagccaatc atgacccaat taggtgctcg acgtttaacc gctgacatct
     1861 tcgccgattc ccatcgcatc tccacgcaga tgtccttgcg cggccgcgtg ttgatcgata
     1921 tattgaatga tcccaacacg tcttatttgg acctcgatat cgcttacgtc tcgcgcatcg
     1981 atcatcccgg cgaaattctg gccgattatt ccttatcgat cgtacggaaa gatcgcctaa
     2041 ctttcatcgt cgtcgccacc atgggcgaaa tggcgctgaa acaaaatccg gctttgaaag
     2101 tgggcatgca gctgtggccg gtctttgtga ctgtgcccgg ctttgagatc accggacaga
     2161 ttgcagcgcc ggccaacctc gatctgcgca cgctgatggc catcggtggg gaacgcttca
     2221 tgccggtctt cgacgccaag gcaacattgt cgatgaatca gtcggtggag tttggcggcg
     2281 aattggtgtt gatcaacaag tccgcgatcg aagtgctctg tgtcagcaat aagctcgaac
     2341 gataatcgga gtaaaccgaa catggcgacg attctagtaa tcgacgacga tcttgatttg
     2401 cagcaaatgc tgcgcttgat gctgcagcgc ggcggccaca aagtgatcac caccggcgac
     2461 ggcggcgatg gcctcgcgaa agctaagcaa ctccatcccg agctggccat cgtcgatgtg
     2521 atgatgccgg gcatgaacgg ctatcaggtc gtgcgcaaaa tgcgcgcaga gcctgaaacc
     2581 gccaacacgg ccattctaat attgaccgcg cgcgcccaga cggtcgatcg tgaagccgcg
     2641 ctggccgctc acgccgacga ttatttggca aagccggttg caccgaacga actattaacc
     2701 aaagtgaatg aattgctggc acagcgcaag gcctcccaag gtccggctca ggaaaccatc
     2761 gcactgatgt cgctgcgcgg tggcgttggc gttacttcaa tagcggttaa cctcgccctg
     2821 gcgtggcaac tgaagaaccg cgccacctgc ttgatcgatc ttaaacccgg ccccggccac
     2881 atcgccttgc acttgcgcct aaacgcgaag accacttgga tcgattgggc taacagcggt
     2941 gatacaccga tcgacgtact gtcgaattat ttgctgccgc atgattccgg cttaagcgtt
     3001 ctagctgccc cgattgttcc ggcggacgtc cccgtgcccg atcgggttgc gaagctgatc
     3061 gggctattgc gagaaaaatt cgcacgggtg gtgatcgata tgccttcgaa ctgggatgta
     3121 ctcggcgctt cgatcgcaac cgcttcggat gcgatctggc tggtcattgc gccggaagtc
     3181 ggctcactac aatcgaccgt gggcgccatg cgcgtgttaa agaccactaa gattcccgac
     3241 gagcgcgttc aactaatcgc gaatcaagtc atgcccaaac ccggcttggc gatgcaagcg
     3301 attgaaaaag ccctggggca tggctttaaa ggcagcctgc catatgatga agcacaatcg
     3361 gcggccattg cgcagggact gccgttaatg accagccaat caagttcacc tctcgctgtg
     3421 gcgattcgat cgctgaccgt ctgatccatc tatggttaaa cgcgccgctc gatcgaaagc
     3481 agcttccccc gaaacaccac gcgaaacggc gctggcgctg atcgaattcg atagcatcgc
     3541 caccggcctg caagcgggcg atgcgatgat caagcgcgcg ccgatcccac gcatcgtcgc
     3601 cggcacggtt cagcccggac attatttggt tttaatcagc ggcacagtcg ccgacgtgga
     3661 agagtcgttg aaagcgggcc gcgaggtcgg cggctcgaat gtcgtcgata ctgtttactt
     3721 gccgcaagtt cactccgcgg tcgttgatgc aattgccggc ggacgcaacc ctcagcccgg
     3781 cacggcttta ggaataatcg aaacctcgac cgttccggcg gcgatccaag cggccgatgc
     3841 gggcgtgaaa ggcgcgcagg tcgcgctgct tgagattcgc ttggccgatg gcctcaacgg
     3901 caaaggtctc acgctgttca ccggtgatgt cgcggatgtc gaagtcgcaa tcgagatcgg
     3961 cgtgaagtcg atcgatgcgc gcatgttgac aaacaaggtc atcatcccgc agattcatcc
     4021 cgagatgaac gcaaatgtaa tcgagcgtac gcgctttgga tcgaacgtca gtgagtattg
     4081 gcctctcacc aatgacgagt gagcaccttt aggcatgtca tttcgagccg catcatttcg
     4141 cggcgagaaa tctctacttc gatttactct tgatgctaat tgtcgcagag atttctcggc
     4201 cgacggcctc gaaatgacca caatgagaac ctatgcaact ggctaaagtc accggcacac
     4261 tcgttgccac ccaaaaatac gatggccttg agggcgtcaa gtttttgatc gtccagccat
     4321 tgggcaaaca tcaaaagccc gaaggcgatc ccatcgttgc ggccgacggc actgcgcaag
     4381 ccggaccggg catgaccgtc ttcgtcgtcg cctcgcgcga agccgccctc gccctgcccg
     4441 tcaaattcgt tccggtcgat catgcgattg tggggattgt cgatcaagtg gatatcgcta
     4501 agtcacaccg agctgagtga tatcctaaga ctttgtcgga tataagcaat tatcctgcag
     4561 tcggataaat cgcccatatg gggtattatc cgacatagac gttggataat ccctagttgg
     4621 gcagacgagg cgcggatgag cagacggatc attctcagcg agaggcgaga ccctaggcta
     4681 gtaaccgtgc gccgcggagg cacgctacag g
//
LOCUS       DASRTJ010000266         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_318076,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000266 DASRTJ010000000
VERSION     DASRTJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318076"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VFF70_05470"
     CDS             complement(<1..1590)
                     /locus_tag="VFF70_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44185.1"
                     /translation="MLIIVIAFDLAIIFYRRRIKRRAALTGIATSGQPSFIARFSFKP
                     RPTLDKSLFVIALLVFLATRLIGLEKFPIYFFTDEAVNTVNAADFIDNGFRDPEGHLF
                     PTYFQNDQIRSLSVSVYIQVIPYLLFGYSIFVTRAASVLIALSAMFALGLIFKKCFKL
                     RYWWLGVFILSVTPAWFLHTRTAFEAVLWVSFYVWFLYFYLQYRQGQLRNIFGALLTG
                     ALSFYAYNGGQLGVVLTGSLLVLIDRKYHWQVLQRHRRMMLLALSFGIILMLPYLRFM
                     SQFPLEISQHLRLLGSYWQPGVPFIDKLGGFAREYLFGLNPIYWYSTINDHDLIRHIM
                     KGYGNSLWITLPLAVIGLIICLRKLRAPEYRTILIALIVSPVGGALSDATVVHDLLFV
                     IPAAVLTTIGAVKVLSLLQQRIAYPALAISAAAILSVFNVLMLQDALSNGPTWYDNYG
                     LYGLQYGGKEVFQAMNTYLQQSPHIDFWVFPQTWLNGPDAIQRYFVPSGANVSMFDFD
                     DFLLQRYDVLPTDLLVVASDQY"
     gene            complement(1638..>2063)
                     /locus_tag="VFF70_05475"
     CDS             complement(1638..>2063)
                     /locus_tag="VFF70_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44186.1"
                     /translation="RRQPFQEDITLDGQSVHVLHSRFDSGRLVDLFDGDTFTLARTAI
                     DNPAFLEFTFDQQRSMSGITLTTGTLDFLLTVSVWTDENSAPKIYPHSFTGLPPDPTV
                     SVDFGSAFAVKKLRIEVRDIRAGTDNHIHIREIQFRRSQ"
BASE COUNT          553 a          509 c          569 g          432 t
ORIGIN      
        1 atattgatcg ctggcgacga ccagcagatc ggttggtagt acgtcgtatc gctgaagcag
       61 aaaatcatcg aagtcaaaca tggacacgtt ggctccactg ggcacgaagt accgctggat
      121 cgcatctggc ccgttaagcc atgtttgcgg gaaaacccag aaatctatgt ggggcgactg
      181 ttgcagataa gtattcatgg cttgaaatac ttctttgcca ccatattgca gtccatacaa
      241 accgtaattg tcgtaccacg ttgggccgtt gctcaatgca tcttgcagca tcaacacgtt
      301 gaatacggac aagatagccg ctgcactgat cgcgagcgca gggtaggcga tgcgctgctg
      361 cagaagtgag agcaccttca ctgcgccgat cgtcgtcaac actgcggcgg ggatgacaaa
      421 caacagatcg tgcaccaccg tcgcatctga gagtgcgccg ccaactggcg aaacgattaa
      481 cgctatcagg atcgttcggt actccggcgc acgaagtttt cgcaagcaga tgatcaagcc
      541 gatcacagcc aaaggcagcg tgatccacag gctgttgcca tagcctttca tgatatgccg
      601 gatcagatcg tgatcgttga tggtcgagta ccagtagatt ggattcaggc caaatagata
      661 ttctcgcgcg aagccgccaa gtttatcgat aaaaggcacg cccggttgcc agtatgaacc
      721 gagcagccgc agatgttggc tgatctccag cggaaattgt gacatgaaac gcaggtacgg
      781 cagcatcaat atgatcccga aggataatgc cagcagcatc atgcgccgat gccgctgcag
      841 gacttgccag tgatacttcc gatcgatcaa cacgagcagc gaaccggtga gcactacgcc
      901 caattgtccg ccattgtaag catagaaact caaggcaccg gtcagcagtg ccccaaaaat
      961 attccgcaat tggccttgcc gatattgcag atagaagtaa agaaaccaga cgtagaatga
     1021 aacccatagc acggcttcga aagcggtgcg agtgtgtaag aaccaggcgg gcgttacgga
     1081 aaggatgaat acaccgagcc accagtatct tagcttgaaa cacttcttga atatcaaccc
     1141 cagcgcgaac atcgcgctca acgcgatcaa taccgacgcc gcgcgcgtca cgaagatcga
     1201 atagccgaac aggagatagg gaatgacctg gatatacacg ctcacggata ggctgcggat
     1261 ttggtcgttt tgaaaataag tgggaaagag atgaccttcg ggatcgcgga agccgttgtc
     1321 gatgaaatca gccgcgttca cggtattgac ggcttcgtcg gtgaagaagt agatcgggaa
     1381 cttttccaaa ccgattaagc gcgtagccag gaaaactagg agcgcgatca caaacaagct
     1441 tttgtctagt gtcggccgcg gcttgaagct gaatcgggct ataaatgatg gctgacctga
     1501 tgtggcaatt ccagtcaaag cggcacgacg tttgatccgc cgccgataaa aaatgatggc
     1561 gaggtcaaac gcgatgacga tgatgagcaa taggaggact ggtaaattga tcaacgacat
     1621 tcgatttcgc tgacggtcta ctggctgcgt ctaaactgaa tctctctaat gtggatatga
     1681 ttatcagttc cggcgcggat gtcgcgcact tcgattcgaa gcttcttcac cgcgaaggcc
     1741 gaaccgaagt caacgctcac tgtgggatcg ggtggcaagc cggtgaacga gtgcgggtag
     1801 atctttggcg cagagttttc atcagtccag acactgaccg ttagcaagaa atccagtgtg
     1861 ccggtggtga gtgtgatgcc gctcatcgat cgctgttgat cgaaagtaaa ttccaggaaa
     1921 gccggattat cgatcgctgt gcgtgccagg gtgaacgtat cgccatcaaa caaatcgacg
     1981 aggcgtcccg aatcaaagcg cgaatgcaag acgtgcacgc tttgtccgtc gagcgtgatg
     2041 tcttcctgga aaggctggcg ccg
//
LOCUS       DASRTJ010000267         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_53418,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000267 DASRTJ010000000
VERSION     DASRTJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53418"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..102)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(157..1047)
                     /locus_tag="VFF70_05480"
     CDS             complement(157..1047)
                     /locus_tag="VFF70_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZY44187.1"
                     /translation="MTDQSHSAVTIETIDDFYSFHLDNTRPIEIYLPPDYSGDPQRKY
                     KVLYANDGQDLRGLSLNSILDALYARHAIDPIIVVAIHATNDRMNEYGLSDQPDYRQR
                     GSQATTYAHFVIDELMPFIDRHYRTLGGSMNTAIMGWSLGGLSAFDMAWQRPDKFGIV
                     GAFSPSFWWRSDNSSPRSQQASRLAHRIVRAGIKRPGLRFWFQTGTRDESADRDRNGM
                     IDSIQDTIELIAALKSIGYRSNREVKYVEVKDGRHTQETWAQALPLFLHWVFPQPPNR
                     FVRWIDRVVNLARRNPRIGY"
     gene            complement(1114..2130)
                     /locus_tag="VFF70_05485"
     CDS             complement(1114..2130)
                     /locus_tag="VFF70_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44188.1"
                     /translation="MPPDKIDAQICYLLTTPCDTPVPLNAPESIRPLKDAPYFTPIDI
                     VIRALDSETIELENVAVTIRRQVYDGLTQVAECFYALPDVLSRDAFHLKARIQTALRA
                     HLHGAGEISEEYTALLVTQFDGTPAEFIERNGQSLARFLRSQRERFDPQEISDVVMSR
                     ARYSESELTVVDWDGAIVISPDGDFQSDIELFKIGNYQLLRYRLLDQAIERNLRTVND
                     RLLTGARSLLPNRSRRIMRESIEQRLAIMLEFEKIDQNLLLIGDWYTAKLYQIIYDEF
                     YLDEWKSAIQSKLGNLESINQLIQDNLTFSWSSFLEFMQIVGWLILLVGYFVLFFLEA
                     SKGR"
     gene            complement(2130..2552)
                     /locus_tag="VFF70_05490"
     CDS             complement(2130..2552)
                     /locus_tag="VFF70_05490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZY44189.1"
                     /translation="MSLENFRFSIPVHIRLSDLDSFGIVNNAVYFTYMEEARTEYLIR
                     LNLIREIAVDPGFIIAEATCQFKVPIRFEQSIVVKARVDHIGTSSFSMLYQMEDQATS
                     QIVATGRTTNVAYDYSTCCSMPLLDTWRAAIEKFEAGP"
     gene            complement(2610..>3181)
                     /locus_tag="VFF70_05495"
     CDS             complement(2610..>3181)
                     /locus_tag="VFF70_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44190.1"
                     /translation="AISLYPFYDGGDFFGLKWKFILYTTSLPSVAQAALQDSIGLDAS
                     RLLVNRASMILLAVVVLVQTWRAYRSYDWRMTLRAALIILLFYLLVSAAWFQAWYIEW
                     VLVLAAMLVGSGLSRLTLLLSYSVMWKSIFIDYYLYNGNWIDARASRELLTIIVIFGV
                     VWVYGAYLIGRRIFDRADSPQRSSALEIP"
BASE COUNT          792 a          844 c          848 g          697 t
ORIGIN      
        1 ccggaattgg cacctggcgg ctgcaaaagc gatcatcact ttcacaaccc gctggttgtc
       61 gaggcatcat cgggccggtc cctccgcctc tctggataag agtcattcta tttaattgtt
      121 gtccgcgcga gagaatacca cacttccaaa atgcggtcaa tagccgattc ggggatttcg
      181 ccgggctaag tttacaaccc gatcgatcca tctcacgaac ctgttggggg gctgcggaaa
      241 gacccagtgc aaaaagagcg gcagcgcttg cgcccaagtt tcttgagtgt gtcgaccgtc
      301 tttcacttca acatacttca cttctcgatt agatcgatag ccgatcgatt tcagcgcagc
      361 gatcaattcg atcgtgtctt ggatcgaatc gatcatgccg ttgcgatctc gatcggcgct
      421 ctcgtcgcgc gtgcccgttt gaaaccagaa ccgcaaaccc ggccgtttga tcccggcccg
      481 aacgattcga tgcgcaagtc gcgaggcttg ctgcgagcgc ggcgatgaat tatccgatcg
      541 ccaccagaac gaaggcgaaa atgcaccaac gatcccaaat ttgtccggac gctgccacgc
      601 catatcgaat gcagacagtc cgccgagcga ccagcccatg atcgccgtgt tcatcgagcc
      661 gccgagggtt cgatagtgac gatcaatgaa gggcatgagc tcgtcgatca caaagtgcgc
      721 gtaggttgta gcttgtgagc cgcgctgccg ataatcaggt tgatcgctca ggccatattc
      781 attcatccga tcatttgtcg cgtgaatcgc cacgacgatg attggatcta tcgcgtgacg
      841 cgcgtagagc gcgtccaaga tactatttaa agagagaccg cgcaggtcct gaccatcatt
      901 ggcatatagg actttatact tccgttgagg gtcgccggaa taatcgggcg gcagataaat
      961 ctcgatcggg cgcgtgttgt cgagatggaa ggaatagaag tcgtcaatcg tttcgatcgt
     1021 tacggcggag tgggattgat cggtcatgac tgtcgagacg cgtccttcga cttcgcggca
     1081 aaaggatgcc gctccgctca ggatgctgca ttgctatctg cccttgctcg cttccaagaa
     1141 aaacaataca aagtaaccaa ccaacaaaat caaccagccg acgatctgca tgaattccag
     1201 gaaactcgac cacgaaaaag tgaggttgtc ttgaatgagt tgattaatcg attccagatt
     1261 gcccagtttg gattgaatcg cgctcttcca ttcgtcgagg taaaactcgt catagatgat
     1321 ctggtacaac ttggcggtat accaatcgcc aatgagcagc aggttctgat cgatcttttc
     1381 aaattcgagc atgatggcca agcgctgctc gatcgattcg cgcatgatgc gccgcgatcg
     1441 atttggcagg agcgatcgcg cgccggtcag caagcgatcg ttcaccgtgc gtaggttgcg
     1501 ctcgatcgct tgatcgagta atcggtagcg gagcagctgg tagttgccga tcttaaacag
     1561 ctcaatgtcg gattggaaat cgccgtcggg cgagatcaca atcgcgccgt cccaatcgac
     1621 gacggtgagt tcactttcgg aatagcgcgc gcgcgacatg accacgtcgc tgatttcctg
     1681 cggatcgaag cgttcacgct gcgagcgcaa gaagcgcgcc aaagattgtc cattgcgttc
     1741 gataaattcc gcgggcgtgc catcaaattg agtgacgagc agcgccgtgt attcttcaga
     1801 tatttcgccg gcaccgtgca agtgcgcgcg caaggcggtt tgaatgcgcg ccttgagatg
     1861 aaacgcgtcg cggctcaaga catccggcag cgcgtaaaaa cattcggcga cttgagtcaa
     1921 gccatcatag acttgacgcc gaatcgtcac cgccacattt tctaattcga tcgtttccga
     1981 atccagcgcg cgaataacga tatcgatcgg cgtgaagtac ggtgcgtcct tcaatggccg
     2041 gatcgattca ggcgcattca acggcacggg tgtgtcgcag ggcgtcgtga gcagataaca
     2101 aatctgcgca tcgatcttat cgggtggcat taggggcctg cctcaaattt ttcgatcgcc
     2161 gcgcgccagg tatccagcaa aggcatacta cagcatgtcg agtaatcgta cgcgacatta
     2221 gttgttcgcc cagttgctac gatctggctc gtggcttgat cttccatttg atacaacatg
     2281 ctaaaactcg aagtgccaat gtgatcgaca cgggctttga cgacgatcga ttgctcgaag
     2341 cggatcggca ctttgaactg gcaggtcgcc tcggcaatga tgaagccggg gtcaacagcg
     2401 atctccctaa tgagattgag ccggatcaag tattcggtgc gcgcttcttc catgtacgtg
     2461 aagtacaccg cgttgttgac aataccgaac gagtcaagat cgctcaagcg aatgtggaca
     2521 gggatggaga agcggaaatt ttctaatgac aagatatatt ggctttcatc gatggtgctg
     2581 cttgtgatta taccagcacg gacgccgcat caaggaattt ctagcgcgct cgaacgttga
     2641 ggggagtccg ctcgatcaaa tattcgccgc ccgatcaagt atgcgccata cacccacacg
     2701 acgccaaata tgacgatgat cgttagcaat tcacgcgagg cgcgcgcatc gatccagttg
     2761 ccgttgtaca aataatagtc gataaagatc gacttccaca tcaccgagta actgagcaac
     2821 aacgtcaagc gcgatagacc actgcctacc aacatcgccg cgaggaccaa tacccactcg
     2881 atataccacg cttgaaacca cgccgccgat acgagcaaat aaaacaagag gatgatcaac
     2941 gcggcacgca gtgtcatgcg ccaatcgtaa ctgcgataag ctcgccacgt ttgaaccaag
     3001 acaactaccg cgagcaagat catcgaggcg cggttgacga gtaaacggct ggcgtccaag
     3061 ccgatcgagt cttgcagcgc ggcttgcgcc actgacggca gcgaggtggt atataaaata
     3121 aatttccact tgaggccgaa gaaatcgccg ccatcataaa acggatagag agagatggca
     3181 a
//
LOCUS       DASRTJ010000268         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_53522,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000268 DASRTJ010000000
VERSION     DASRTJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53522"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFF70_05500"
     CDS             <1..735
                     /locus_tag="VFF70_05500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44191.1"
                     /translation="ELGCALRQIVWDRLAANFNSGDRILELNCGTGEDAIWLARRDVR
                     VTATDVSLEMLAIARKKISEAELTQRVDLQPLDLAQPVHRPAASFDGALSNFGGLNCV
                     NNLRPLSKFLGEAIRPGGELILVPMNRWCVWEIVWHLLHFKFRTAFRRLRSAGSEANV
                     GANRARVWYPSIRSLRMTFEPHFKLKRITGLGIFLPPSYLERSMARHPKLFSRLNRLE
                     QKFDRVFPFNRLSDHVILEFERTASA"
     gene            732..1445
                     /locus_tag="VFF70_05505"
     CDS             732..1445
                     /locus_tag="VFF70_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44192.1"
                     /translation="MNHPIKWITRLLNPPIRSLELEPREAYRLWAENYPPRPHNLLMQ
                     VEQQVMFAQLPDVRDRRALDLACGTGRYAVLLKERGARVIGCDLSYEMLRHADVNLRR
                     AQSDMLRVPLASGCADIIVCGLAIGHVQDLSMALIEMARVLVTGGVALYSDFHPLSRD
                     LGWQRTFRASTGRQFAVQFVTHTLESHRRAAQAAGLSIEMTEVHVDQELATTNADAAW
                     YRSRWGDTPVALVIRAHKN"
     gene            1448..>1642
                     /locus_tag="VFF70_05510"
     CDS             1448..>1642
                     /locus_tag="VFF70_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZY44193.1"
                     /translation="MWYSPSIYFDNARLLDPEQGDSLTSIRLAHGRVAGLGVKAQSGD
                     RRFDLSGAVALPGLINAHDHL"
BASE COUNT          340 a          406 c          493 g          403 t
ORIGIN      
        1 gaattagggt gtgcgctgcg ccaaatcgtg tgggatcgat tggcggcaaa cttcaactcg
       61 ggcgatcgca ttctagaact aaactgtggc acgggcgagg acgccatctg gttggcgcgg
      121 cgggatgtgc gcgttacggc gactgatgtt tcgcttgaaa tgctggctat tgcgcgcaaa
      181 aaaatatctg aggcggaatt aacccagcgg gttgatctac agccgcttga tttagcgcag
      241 cccgttcata ggccagcggc ttcattcgat ggcgcgctgt ctaactttgg cggactgaac
      301 tgcgtgaaca atctgcgacc cttatcgaaa tttttaggtg aagcgatcag gccgggcggc
      361 gaattgattt tagtgccgat gaatcggtgg tgcgtctggg aaatcgtttg gcatctactt
      421 catttcaaat ttcgtacggc ctttaggcga cttcgctctg cgggcagtga agcgaatgtc
      481 ggcgcaaata gagcgcgcgt gtggtatccg tcaatccgat cgctgcgaat gacattcgag
      541 ccgcacttca aactgaaacg aattaccggc ttgggaatat tcctaccgcc atcttatctt
      601 gaacgcagta tggcgcggca tccgaaattg ttcagccggc tcaatcggtt ggagcagaaa
      661 ttcgatcggg tttttccgtt caatcgatta agcgatcacg tcattttgga atttgaacgg
      721 actgcatcag cgtgaaccat ccaatcaagt ggatcacccg cttgctgaat ccaccgatcc
      781 gatcgctgga attggaaccg cgcgaagcgt atcggttgtg ggctgagaat taccctccgc
      841 gcccacacaa cttgctgatg caagttgaac agcaagtgat gtttgcgcag ctgcctgatg
      901 tgcgcgatcg gcgcgcgctt gatttggcgt gtgggacagg gcgctacgct gtactattga
      961 aggagcgcgg cgcgcgtgtg attggctgtg acttgtctta tgagatgctc cgtcacgcgg
     1021 atgtaaatct tcgccgcgcg caaagcgata tgctgcgtgt gccgttagcc agtggttgcg
     1081 ctgacatcat cgtgtgcggc ctcgcgatcg gtcatgtgca agatttgtcg atggcgctga
     1141 tcgaaatggc gcgcgtgcta gtgacgggcg gggtcgcgtt gtattcggac tttcatcccc
     1201 tgagccgcga tctcggctgg cagcgcacat ttcgcgcttc gacgggcagg caattcgccg
     1261 tccagttcgt gacccacact ctcgaatcac atcgtcgggc ggcgcaagcg gctggcttgt
     1321 cgattgagat gactgaagta catgtggatc aagaattggc aacaacgaac gccgacgcgg
     1381 cttggtatcg atcgagatgg ggcgatacgc cggtggcttt agtcatccgc gcgcataaga
     1441 attgatcgtg tggtattcac cctccattta ttttgacaat gcccggctgc tcgatcctga
     1501 gcagggcgat tctttaacta gcattcgttt agcacacggt cgagtggcgg gactgggcgt
     1561 aaaagcacaa tctggtgatc gacggtttga tctgtcgggg gcagtagcct tgccgggatt
     1621 gatcaatgcg cacgatcatc tg
//
LOCUS       DASRTJ010000269         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_347175,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000269 DASRTJ010000000
VERSION     DASRTJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347175"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFF70_05515"
     CDS             <1..191
                     /locus_tag="VFF70_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZY44194.1"
                     /translation="DILEALKVDPIVWRNFKKFPKSYQHIRVGWIDGARKRPIEFQKR
                     LRYFIKMTAQNKKFGMVQ"
     gene            212..955
                     /locus_tag="VFF70_05520"
     CDS             212..955
                     /locus_tag="VFF70_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HZY44195.1"
                     /translation="MQAIILAAGVGRRLQPFTYDRPKCLARFGGQSLLARHLNLLRAF
                     GITQINLIVGYFADQIKTEAATQDLHIPIEFILNPAYERGSAVSLIAAQKILRNHSTL
                     VMDADLLYDQNILRLIVETKFENALLVDRLLDDTGEEVKAIVREQRVWGLGKHLSTPG
                     HVAGESLGIFKFSPRAGTLLADGLQGLIDQSGLDTEYETAIHSSLPEYRMDYIDIDGL
                     PWIEIDTPADLQRAEASIFPTLEKFNRRS"
     gene            957..1631
                     /locus_tag="VFF70_05525"
     CDS             957..1631
                     /locus_tag="VFF70_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HZY44196.1"
                     /translation="MTKLAEWYYTSESFPARRLRSMRRGLRVIKKNFVALNFVQRRRF
                     KRNLVRLHDALAQTDFAHKYWVIDGLLLGWARTGQIIPWDTDDADFAYLGEDRSAFAQ
                     ARAKLSEAGFSLSSDWRNNAGEITATVFTRDGIRFDFFEMQPQGNQLLYTLYGRRNGI
                     WQELKCAVPRHGLADMPFLDRIWQKPDDHDIFLRAVYGEWRKSDPGYDYASDEKNVVG
                     VTPRKL"
     gene            1759..2118
                     /locus_tag="VFF70_05530"
     CDS             1759..2118
                     /locus_tag="VFF70_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44197.1"
                     /translation="MDENAAANELNEDEWRHATSSNPAFDSLKETAEDIYTVMNAQTE
                     AQVAEDEQIRQEHSLVIDGVIAIPTDIDPDAFFDRLLDKLIEYVEEHHGYAGLGMSHK
                     KYEGEAEDELADGSQAT"
     gene            2099..2365
                     /locus_tag="VFF70_05535"
     CDS             2099..2365
                     /locus_tag="VFF70_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44198.1"
                     /translation="MAAKQPKTGQEFVDLAKKSEKVSNVRTGKGSHVVVEFKNGSSIT
                     VPIHGHQQLGRGILHKITKAFKAAGALLIILMLLWIGSRFFLFP"
     gene            complement(2462..2698)
                     /locus_tag="VFF70_05540"
     CDS             complement(2462..2698)
                     /locus_tag="VFF70_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZY44199.1"
                     /translation="MQGAIVKMQIAGSTDMNGLHKELHAVENVKEVYFLAGPTDVMCH
                     VEAMDVDAVVSTVVKIRGMKGVTSTDTRFILPLH"
     gene            3044..>3377
                     /locus_tag="VFF70_05545"
     CDS             3044..>3377
                     /locus_tag="VFF70_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44200.1"
                     /translation="MKRILFAIFVIMIALVGCQSAPPTTPPPPPTATPTPLPPGITGV
                     VLYTGTQKGGLLILAMNQPPKPNEPPQAVKGATFADVSSGEFVWDLPPATYYLIAFFT
                     IGRTPTGPT"
BASE COUNT          827 a          866 c          891 g          793 t
ORIGIN      
        1 acgatatcct cgaggcattg aaagtcgatc cgatcgtttg gcggaacttc aagaaattcc
       61 cgaaatcgta tcaacacatc cgcgtcggct ggatcgatgg cgcgcgcaaa cgcccgatcg
      121 agtttcagaa gcgtttgcgc tacttcatca aaatgacggc gcaaaacaaa aagtttggaa
      181 tggttcaata aaacgggggg aaacctgatc gatgcaagcc attattctag cggccggggt
      241 cgggcggcgg ctgcagccct tcacttacga tcgaccgaaa tgtcttgcac gcttcggcgg
      301 acaatcgctg ttggcgcgtc acttgaattt attgcgggca ttcggtatca cacagatcaa
      361 tctcattgtc ggttatttcg ccgatcagat caaaacagaa gccgccactc aggatttgca
      421 catcccgatc gagttcatcc tgaacccggc ctatgaacgc ggcagcgcag tatcgttgat
      481 cgccgctcag aaaattctcc gaaatcattc cacgctggtc atggatgccg acttgttgta
      541 cgaccagaac atcctgcgct tgatcgtcga gacgaaattt gaaaatgccc tgctggtcga
      601 tcgacttctg gacgacaccg gtgaagaagt gaaggccatt gtccgcgagc aaagagtgtg
      661 ggggttggga aaacacctct cgactcccgg ccatgtggcc ggtgaaagtt tgggtatctt
      721 taagttctcg ccccgggcgg gcacgctgct cgcagacggt cttcaagggc tgatcgatca
      781 atcggggctc gacactgagt acgaaaccgc catccactcc tcgctgccag aatatcgcat
      841 ggactatatc gacattgacg ggctgccctg gatcgagatc gatacgccag cggatttgca
      901 gcgggccgag gcgtcgatct ttcccacgct ggagaaattc aatcggcgat cttaagatga
      961 cgaaacttgc ggagtggtat tacacttcag agtcgtttcc ggcgcggcgg ctgcgatcga
     1021 tgcggcgcgg cttgcgggtg atcaagaaaa atttcgtggc gttgaatttt gttcaacgcc
     1081 ggcgctttaa gcgaaatctc gtccggctgc atgacgcgct tgcgcaaacc gattttgccc
     1141 acaagtattg ggtgatcgat gggctgctcc tgggctgggc ccgcacgggg cagattattc
     1201 cgtgggatac cgatgatgcg gattttgcct acctcggcga agatcgatcg gcgtttgccc
     1261 aggcacgcgc gaaactcagc gaagcgggtt tttcattgtc gtcggactgg cgaaataatg
     1321 ccggagagat tacggctacg gtcttcacgc gcgacggaat ccgcttcgat tttttcgaaa
     1381 tgcagccgca aggtaaccaa ttgctttaca cactgtatgg tcggcggaac gggatttggc
     1441 aagaattaaa gtgtgcggtg cccaggcacg gcttagccga tatgccgttt ctcgatcgaa
     1501 tctggcagaa gccggacgat catgatattt tcctccgcgc cgtttatggt gaatggcgca
     1561 agtcggatcc cggctacgat tatgccagcg atgaaaagaa tgtcgttgga gttactccgc
     1621 gcaagttgta atcagcgcgt cggctggatc gatggggcag gataacaaag tggggcgatc
     1681 gaagttcgat cacccgttta cttctcgcgg gcttgatgta taatctcttc aagacgatcg
     1741 gtggaggagt aatgaccgat ggatgagaat gcggcggcaa acgaattgaa tgaagatgag
     1801 tggcgacacg ccacctctag caatccagcc ttcgattctc taaaagagac ggcagaagat
     1861 atttacacgg tgatgaacgc gcaaaccgaa gcacaggtcg ctgaagacga gcagattcga
     1921 caagaacata gcctggtgat cgatggcgtc attgcaattc caactgacat tgatccagac
     1981 gcatttttcg acagactact cgataagctt atcgagtacg tcgaagaaca tcacgggtat
     2041 gcaggtctcg gcatgtcgca taaaaaatat gagggtgaag cggaagatga attagccgat
     2101 ggcagccaag caacctaaaa ccggacagga atttgttgat ctggcaaaga aatcggagaa
     2161 ggtcagtaac gtgcgaaccg gaaaaggctc gcacgttgtt gttgaattca aaaacgggtc
     2221 atcgatcacc gttcccattc atggacatca gcaattaggc cgaggcatcc tgcataaaat
     2281 tacgaaagcc ttcaaagcag cgggcgcact tcttatcatt ctgatgctgc tctggattgg
     2341 ttctcgcttc tttctgttcc cataactttc ggcctgcggt tgtctacttc tcaatcagtg
     2401 tgaacaaaat tagggcgatc gaaattcgat cgccccagtt tgtaatcaac agtcgctact
     2461 ctcagtgaag cggcaagata aaccgcgtat ccgtactcgt tacgcctttc atgccgcgaa
     2521 tcttcacgac agtgctcacc acggcatcga catccatggc ttcgacatgg cacatgacgt
     2581 cggttggccc agccaggaaa taaacttctt tgacattttc cacagcatgc agctctttgt
     2641 gcaatccgtt catatcggtg ctgccggcga tctgcatttt cacaattgcg ccttgcatga
     2701 ttagcctccc tatcttcgta tatggattgg attagttttt acagtgaata gttcgttcta
     2761 ttcgatcttg aaagagagtt cgttctgcac atcacctccc tgtggcgcgt cttcaatacc
     2821 gaggtctgca actcgacctc aacaacaatt gtagcacgaa gtcatcggcg tggtgtcaaa
     2881 tacgagcgac ccttgttcag tcgttgcaac tgcttcgcgc agacttgata cagcaatttg
     2941 actttgagca tgattcgact acactctaga tcaagccgtg gcaacgaaca gaaagtctgg
     3001 tgtcgctcgg aggggatgcg ctacgtcaaa gaggaggatt acagtgaaga gaattctttt
     3061 cgcgatattc gtcatcatga tcgcactggt tggatgccag tctgcgccac cgacgacgcc
     3121 gccaccgcca ccaacggcga caccgacgcc tctacccccg ggcatcactg gagttgtact
     3181 atacacgggc acacaaaaag gcggcctgct catattagcc atgaatcagc ccccgaagcc
     3241 gaacgaacca ccgcaggcag taaagggcgc aacattcgcc gatgtctcgt ctggtgaatt
     3301 cgtttgggac ttgccgcctg ctacctatta cctgatagcg ttcttcacaa ttggtcgcac
     3361 tccgacgggt ccaacat
//
LOCUS       DASRTJ010000270         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_90815,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000270 DASRTJ010000000
VERSION     DASRTJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90815"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1556)
                     /gene="secD"
                     /locus_tag="VFF70_05550"
     CDS             complement(42..1556)
                     /gene="secD"
                     /locus_tag="VFF70_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZY44201.1"
                     /translation="MSQRNRTWTIIIVLLAIIATYIALPSTTTVPLINRNVTVRQGLD
                     LSGGSQVLLEATDCSDPKISERLDNARQIIERRVNGLGVSEPLVQKQGNCRILVELPS
                     IDNPADALALIQQTGRLEFVDAGLDAYKDGTIIRTSRNPTPTIGISSTASAGNIPLSP
                     GLSTSPAITAANSTSSTTNVSSTVPATSTTELLTDTLPTQTIPDKVYQVIMTGADLDA
                     AKLTVQQNSTTREFDVAFTILGEAASTFAQFTTAHNEATSGARYYMCIVLDNVVQSCP
                     SIRTAITGGSGVITIGSGGVDEANRLVSLLRYGALPVALQVVQSRTIGPTLGEDSIRA
                     SVIAGIIGLLAVALFMLFYYRLPGLLADIALLLYTLTVFALFKLIPVTLTLPGIAGFI
                     LSVGVAVDANILIFERTKEELRAGRRLAVAVDVGFERAWPSIRDSNISTLITCVILFT
                     FGNTFGASVVKGFALTLAIGVAVSLFTAIRVTRTLLHLVLDNSDLEERKHLFGI"
     gene            complement(1694..2059)
                     /locus_tag="VFF70_05555"
     CDS             complement(1694..2059)
                     /locus_tag="VFF70_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44202.1"
                     /translation="MDPDHQRQLHIVIVGPCAAGKSTLHNGLHARGFDKARVVAQEHS
                     GVRDLWKMRGRPDCLIYLDARLETIAARQQRSDWTIEYLAELLDRLKNARSASDLYLP
                     TDELSIEEVLDNVIKRLSE"
     gene            complement(2205..2960)
                     /locus_tag="VFF70_05560"
     CDS             complement(2205..2960)
                     /locus_tag="VFF70_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44203.1"
                     /translation="MSRVTAAFVCALDDPDNARPASIMCSNSCGRYVCSEHAIERKGP
                     GSPMVCLVCGRALGVLPIAPKVEVTPTERIVPKVEPLVETIEPPVMPMAEPRRAVRSE
                     SAPMTAAPKVEVKPIEPAARSDPGTTQAGRAFTPPAATFTPEASISPQGAVPVEEQKV
                     APNMIEPEAAPKSVASETIVSQRGNTLQVPKVTSTALHTGQTGPLPKARLAQRTDERA
                     SAGGLQNQAILWAIGAIVLGIIGFALIVAALQG"
     gene            complement(2984..>3534)
                     /locus_tag="VFF70_05565"
     CDS             complement(2984..>3534)
                     /locus_tag="VFF70_05565"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HZY44204.1"
                     /translation="SLLAEGVRDGAVARAASDYLFTQRSGKVSATLNIVSVDLESRSL
                     VLSRNSHCPIVVIKNNEASLLNEPSHPVGVQRGTKPIIAEIPLAADTTVVIYTDGLLS
                     AGERSGQPINIPAIVLELIDHEKQRDTAHTSAQSIADQLLERAVNADEHRPADDISIL
                     VLTITNFEAIDDRRWLIASMPL"
BASE COUNT          776 a          949 c         1005 g          804 t
ORIGIN      
        1 caacccttcc ccattaacac aagggagggc aaggtggagt attaaatccc aaatagatgt
       61 ttgcgttctt ccagatctga gttgtccaag acaaggtgca acaacgtgcg tgtcacccga
      121 atcgcggtga agagactgac cgccacaccg attgccagcg tcagtgcaaa acccttgacg
      181 acacttgcgc cgaaggtatt gccaaaggta aacaaaatca cgcaagtgat cagtgttgag
      241 atgttcgagt cgcgaatcga tggccaggcg cgctcaaagc caacatcaac cgcaacggcc
      301 aatcggcggc cagcacgcag ctcttctttc gtccgttcga aaatcaaaat gttcgcatcg
      361 accgccacgc cgaccgataa gatgaagccg gcgataccgg gcagggtgag tgtaaccggg
      421 atgagcttga acaaagcaaa cactgtcaat gtatataaca gcaaggcaat atcggctaac
      481 aggccaggca atcgatagta aaacagcatg aacaacgcca cggccagtag accaatgatc
      541 cccgcaatca cactcgcgcg gatcgaatct tcacccagcg tcgggccgat ggtgcggctc
      601 tgcacgactt gcaaagcgac gggcagcgcg ccgtaacgga gcaggctgac caatcgattg
      661 gcttcatcga cgccgccact cccgatcgtg atcacgcctg aaccgcccgt gatcgctgtt
      721 ctgatcgatg gacaggactg cacgacgtta tccaacacaa tgcacatgta atagcgagcg
      781 ccactggtcg cttcgttgtg agcagtggtg aattgcgcga aggtgctggc ggcttcgccc
      841 agaatggtga aagccacatc gaactcgcgt gtcgtcgaat tttgctgcac ggttaacttg
      901 gcagcgtcaa gatcggcgcc ggtcatgatc acttgataaa ctttatcagg aatggtttgg
      961 gtcggcagag tatcggtgag cagctcggtc gtcgatgttg ctggcacggt cgatgaaaca
     1021 tttgtggtcg atgatgtcga attggctgcg gtgatggccg gactggtgga caagcccggc
     1081 gagagcggta tgttgcccgc tgaagctgtg ctggagatcc cgatggtcgg cgttggattg
     1141 cggctcgtgc gaatgatggt gccatccttg taggcatcta aacctgcgtc gacaaattcc
     1201 aaacggccgg tttgttgaat caaggccaag gcgtcagctg gattatcgat ggaaggcagt
     1261 tcgactaaga tgcggcagtt gccttgtttc tgcaccaacg gctcgctgac gcctaacccg
     1321 ttcacgcgac gctcgatgat ctggcgtgcg ttgtcaagcc gctcgctgat ttttggatcg
     1381 ctgcagtcgg tggcttctag tagaacttgc gagccgccgc tgaggtccag gccttgccgg
     1441 accgtgacat ttcgattgat gagcggaacg gtggtcgtgc tgggcagcgc gatgtaggtc
     1501 gcgatgattg ccagcagcac gataataata gtccatgtgc gattgcgttg tgacatgcaa
     1561 actccctgac agtacaaaaa agtcgccggc ggaaaaggcc ggcgcgacct gtgaagcgaa
     1621 ggtgattata gtcgaacgga ggtgatctgt caaaagaata cggagtgatc agcgatgcgt
     1681 ggatgttcga ttactattca ctcaaacgct tgatcacatt atctagaact tcttcaatcg
     1741 ataactcatc tgtcggtaaa tacaagtcag acgcagatcg agcgttcttt aatcgatcga
     1801 gcagctccgc taagtactca atcgtccagt cggatcgctg ttggcgcgcg gcgatggtct
     1861 ctaaacgcgc gtcgaggtag atcaaacaat cgggccgacc gcgcatcttc cagagatcgc
     1921 gcacgcccga atgttcctgc gccacgacgc gggccttgtc gaaaccgcgg gcgtgtaagc
     1981 cattgtgcag tgtgctcttg ccggcagcgc atggtccgac gatcacgata tgcagttggc
     2041 gttgatgatc gggatccatt atcggctgcg tctgaagaac gggtttaaac tcggcgattg
     2101 gtttgtggac acttcgcgcc gcgccgttga atgttgcggc gcattgtgtt gttcttataa
     2161 aatgaaagac gcacctttta aggtgcgtca cttcagcatc attatcagcc ttgaagcgcc
     2221 gcgacgatca acgcgaagcc gataatcccc agtacgatcg cgccgatggc ccacaagatg
     2281 gcttgattct gcaagccacc ggcacttgct cgttcatcgg tccgttgagc taatcgagct
     2341 ttaggcagtg gcccggtttg gccggtatgc aacgcggttg atgtcacttt cggaacttgc
     2401 agcgtgttgc cgcgctgcga cacaatcgtt tccgatgcca ctgatttcgg cgcggcttcc
     2461 ggttcgatca tattgggcgc gactttctgc tcttccaccg gcacagcacc ctgcgggctg
     2521 attgatgcct ccggggtgaa tgttgcggca ggcggcgtga acgctctacc ggcctgcgtc
     2581 gtgccgggat ccgatcgggc agctggctcg atcggtttca cttcaacttt cggcgcggca
     2641 gtcatcggcg cagactcaga tcgaaccgct ctgcgtggtt ctgccatcgg catcactggc
     2701 ggttcgattg tctcaactaa tggttcaact ttcggcacga tcctttccgt tggcgtcact
     2761 tcaactttag gcgcgatagg caggacgcct aacgctctcc cgcacaccag acaaaccatc
     2821 gggctgcccg gtcctttgcg ttctatggcg tgctcggaac acacatatcg accgcaactg
     2881 ttgctgcaca tgatactggc tgggcgcgcg ttatcgggat catccaacgc gcacacaaac
     2941 gcagcggtta ctcgtgacat actgacctcc acttcgattt cagttacagc ggcatactcg
     3001 caatcaacca acgtcgatcg tctatggctt cgaagttggt gatggttaag acgagtatgc
     3061 tgatatcatc tgccggtcga tgttcatcgg cgttgacggc gcgttcgagc aattgatcgg
     3121 cgatggattg ggccgaggtg tgggcagtgt cgcgctgttt ctcgtgatcg atcagttcca
     3181 gcacgatcgc cgggatgttt atcggttgcc ccgatcgttc tcccgcgctt aatagtccgt
     3241 ccgtgtatat taccacagtt gtgtcggcag ccagtggaat ttcggcaatg attggcttgg
     3301 tgccgcgctg cacgcccacc gggtgcgatg gttcattcag caaacttgcc tcattgtttt
     3361 ttatgacaac aattgggcaa tggctgttgc gcgacaggac caacgatcga gattccagat
     3421 cgaccgagac gatgttgagt gtggccgaga ctttgcccga tcgctgcgta aacaaataat
     3481 cggatgcagc ccgggcgacg gcaccatcgc gtacaccttc ggccagtaac gaca
//
LOCUS       DASRTJ010000271        10210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_157980,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000271 DASRTJ010000000
VERSION     DASRTJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157980"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFF70_05570"
     CDS             <1..501
                     /locus_tag="VFF70_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HZY44205.1"
                     /translation="GYTMIDRMRQGKKVIVHGDGTSIWVMTHHTDFAKGFVGLLGNPH
                     AIGDAVQIMSDELLTWNQIYNLMAHAAGVQHPQLVHIPSDFIAAFDREWGDSLLGDKT
                     HSVIFDNSKIKRLVPDFVASMPFARGAQEIIHWFDADPARRKIDPALDQTIDRIITAY
                     EAAWPK"
     gene            595..978
                     /locus_tag="VFF70_05575"
     CDS             595..978
                     /locus_tag="VFF70_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZY44206.1"
                     /translation="MPALNRVELIGNLGKDPDARFTANGKKYAAFSVAVNRSWKSTDG
                     KKMEATDWFLINAWGKLGEICLSYLKKGRLVYIDGQLRSEKWEDKDGGETHSRTLIVA
                     QTMQILDRKPNEPEGEETPVEDLAE"
     gene            1166..4327
                     /locus_tag="VFF70_05580"
     CDS             1166..4327
                     /locus_tag="VFF70_05580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZY44207.1"
                     /translation="MSFVPLRVHSQWSLLDGVASIDQIVDHAQALHLPAIALTDTHAL
                     YGVPDFVARCRSAKIQPIIGAEFTVDRTHSIVLLAQNMIGYANLSRLVTHLQARPDRE
                     AALARGLSINDLKQKTDGLIALSGYCNAATDEPYLRELMDLFGNNRLFSELQILDQDD
                     VVQAHALQALVDQLNVQTIATHDIHYLSTADAKKYRVLTAMRLGQRFDDLSASTLEAR
                     DHVEDTRYDAKSTGVSNRDDTVSEPIPLPDYSFPSSEEMQSRFAEFPNALNNTQRIAE
                     LCRFEFPLGQYRFPKLDLPDNRSPREEMWSLAVKGAVDRYKVLTPEIEARLKKETDII
                     DALGYSPYFLVVADIVRFAREHHVPISPRGSASSSVVAYCLGIHDVDPIKHNLYFERF
                     LSLERHDPPDIDMDLCSRRRDEVIDYVYRRYGSDHVAMVCTYATLQSRLALREVCKVF
                     GLSESRIKELSRNLPSYWTPRAQRKAQEAQLIDSARNAVEREAIEISQQLIGTPHHLS
                     IHPGGIVIAPDRITDLVPLQYATKGLLITQFDLKGIEKLGLIKIDLLGISALTVVADC
                     IEFIQQRNPTFSIESSPLSDPETARTLSVAKTIGCFQIESPGMRMTVRELTARTIDDL
                     IVALALYRPGPLKGGLKDAFVRRHLGKEAAEYLHPSLEVILSETYGVIVYQEQVLRIA
                     HEIGGFTLGQADILRRAMSKKSEREMAQLHMEFVEGAQSMSGMTTATAEKVWDLMKVF
                     AGYGFPKAHSAGYGAVAYRMAYLKTHYPAEFMAARLAVWGGFYSPRVYMAEARELGLI
                     VKPPHVNHSDEAFTLDPIDRRTLWMGLGQVRELTHTTIKSIMTQRPFTSIEHFLIRAQ
                     PHHSEVINLIKVNAFEGLGDQEALLSQFDRSHWRGRHSAQLDLMESTTEVKHPERSSE
                     AAGFASQSKDAAITLEQRAKWEQEILGYWVDVHPAQLAAQRTQKIDRVRSNDLNQHSG
                     QEVNLIGVRLAEHRFVDQSGEAWTLIDLEDEVGTFQVLWNEATYRQYRGLLKKREPLL
                     IRGKVRIDRQGQIVVMGSEAKAT"
     gene            complement(4330..5751)
                     /locus_tag="VFF70_05585"
     CDS             complement(4330..5751)
                     /locus_tag="VFF70_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44208.1"
                     /translation="MRRLGCFTPLGIIAGVLTLLIVTGIALASGGSMFSPGALSAQSE
                     GEGALNGVRSHADIGGNCAACHVDPWSSEVMATRCLNCHTDIGQQLNDAQALHGALSH
                     ADQCRNCHTEHNGPQASLTHLNTTDFPHDRVGFSLATHLKLSNGQPFTCIDCHGQRLA
                     EFDRTQCETCHRSYQADFATQHIGDFGNECMACHDGIDRYSKFDHNRLTFTLIGKHTE
                     VACRQCHLNVRSVADYKQAAPTCIACHQKDDQHKGAFGTDCVQCHTSDSWQGAKFDHS
                     LAVFKLTGAHVNVTCQQCHVNNVFKGTPQDCGTCHQKNDQHQGAFGTNCGQCHSTDNW
                     QGAKFDHSVAAFKLTGAHINVHCQQCHINNIFKGTPQSCVACHAEPAVHAGQFGTNCA
                     QCHTTTTWQGATFVHTFPLNHGEGGTIACVTCHTQPANFKVYTCYGCHEHDPIGIQNR
                     HLREGIANFQDCVQCHATGREGD"
     gene            complement(5870..6655)
                     /locus_tag="VFF70_05590"
     CDS             complement(5870..6655)
                     /locus_tag="VFF70_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44209.1"
                     /translation="MRRKTYELYYALAALIAITVVYALVAAQALPRSSSLFGHGIGIL
                     GFLMMLSTEILYSLRKRARGRAIGRMSTWLQIHIFTGIVGPYLVLLHTSWKFNGLAGA
                     LILLTAIIVVSGFIGRYIYTAVPRTVDGAEVAVRDLESQIAVADAKLQALGASRLAST
                     TSVETPGSAALVLERGWLQRKYDREVRRAIKQLDATGQAHAAELRRLLDERYRVQRQI
                     QSLATARRLLAVWHTIHIPIGVALFTVAFIHVGETLYYATFIK"
     gene            complement(6636..7961)
                     /locus_tag="VFF70_05595"
     CDS             complement(6636..7961)
                     /locus_tag="VFF70_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY44210.1"
                     /translation="MARQYVIIGSGIAGLSAAEAIRQHEATAHITLIAEETHGFYSRP
                     GLAYYLRGDIPEKQLFNRSHDDLQALGLRWQKSRAEKILPATHQVLLANGQSVTYDRL
                     LLATGSTTVSPDFPGGELNGVVKLDKLDDARHILKLSGRGRTAVVIGGGITALEIVEG
                     LRERGVRVHYFLRGDRYWSNVLDETESGIVAERLQAEGVIIHAHTHIKQALGKNGKLI
                     GVETQSGERINCQILAIAIGVRPRLELARAAELKIDRGVLVDEFMRTSAPDIFAAGDI
                     AQAYDPRSGRAVLDVLWATAIAQGHAAGANMAGAAKAYTKEVAFNVTQLAGLSTSIIG
                     AVGSGQDEDLVTISRGGSEAWRMLSDAWVVTEREDVNRVRVLVGEQSIVGAFVMGDQT
                     WSRPLYQLIAAQVDIRSIRAAMMDDAAEALTHLTNFYQQWEQHRAPQNV"
     gene            complement(7964..8230)
                     /locus_tag="VFF70_05600"
     CDS             complement(7964..8230)
                     /locus_tag="VFF70_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY44211.1"
                     /translation="MLPDSHSLHFDLKRHEVLAEGQVVPEAIRCVQCGICSYNCPISI
                     DVRAHAWRGLPINDSHCLTCGQCVLRCPRSVLRFERIGTFTVES"
     gene            complement(8622..10166)
                     /locus_tag="VFF70_05605"
     CDS             complement(8622..10166)
                     /locus_tag="VFF70_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44212.1"
                     /translation="MAANSQSISGRRIQQLEMRGIVKRFPGVLANDHVDFDVTAGEVH
                     ALLGENGAGKSTLMRQLYGLYQPDEGQILIDGQPIEFRSPADAIRAGIGMIHQHFMLV
                     PTLTVAENVALGLRSSREPRLDLDRVSERIRSLAQAYGLKVDPKVPVWQLAVGEQQRV
                     EIVKALYRGASLIILDEPTAVLTPQEVDDLFVTLRRMASEGHALIFISHKLHEVTAIS
                     DRVTALRDGRVVGTRPTKGVTRNELAQMMVGREVKPLAPQALHAGEVRLRVDALHARG
                     DNGTEMLRGISLEVRAGEIVGLAGVSGNGQRELAECLAGLRKHTSGSIVLNEQDATNA
                     SLHERMAIGQAYIPEERMRDGAIREFTVQENLFLHDHVSPKYTHGIFMDFGRMASRAR
                     ELVDRFNVKTPRLETPLKNLSGGNIQKLILARELSREPKVLIAAQPTRGVDIGATEYI
                     HHRLLEQRDAGTAILLISEDLDEIQEISDRIAVMYEGRIVGIVDRGAATPEQLGLLMA
                     GVVIEK"
BASE COUNT         2319 a         2874 c         2808 g         2209 t
ORIGIN      
        1 ggctacacca tgatcgatcg gatgcggcag ggcaagaaag tcatcgtgca cggtgacggt
       61 acatcgatct gggtgatgac ccatcacacc gatttcgcca aaggtttcgt cggtctgttg
      121 ggcaatcctc acgcgatcgg cgatgcggtc cagattatga gtgatgaact gctaacgtgg
      181 aatcaaattt acaatctaat ggcgcacgcc gccggagtac aacatccgca gttggttcac
      241 atcccatcgg atttcatcgc agcgttcgat cgtgaatggg gcgacagttt actcggcgac
      301 aaaacgcaca gcgtcatctt cgacaacagc aagatcaaac ggctggtgcc agactttgtg
      361 gcgagtatgc catttgcgcg cggcgcacaa gagatcatcc actggttcga tgccgatccg
      421 gcgaggcgga agatcgatcc ggcgttggat caaacgatcg atcggatcat caccgcgtat
      481 gaagcggctt ggccaaagta aatcgccatt ttgccttttc gcggtgatgt aataaactta
      541 gctcagccgc acggcgtgtg cgatgatcga tcaaacagaa gaaaggggat tatcatgcca
      601 gccttgaatc gagttgaact gatcggaaat ttgggcaaag acccggacgc gcgtttcacc
      661 gcgaacggca agaagtacgc cgcgttcagc gtcgccgtga atcgatcgtg gaaatcaacg
      721 gatggcaaaa agatggaagc caccgattgg tttctaatca acgcgtgggg caaactcggc
      781 gagatttgtt tgagttactt aaagaaaggc cgcttggtct acatcgacgg gcaattgcga
      841 tcggagaagt gggaagacaa agacggcggc gagacgcatt cgcgcacgct gatcgtcgcg
      901 cagaccatgc agattctcga tcgcaaaccg aatgagccgg aaggcgaaga gacgccggtg
      961 gaagatctgg cagaatagat catattaacc gcagagacgc ggaggcgctg agtaaaatta
     1021 ttaaacctct acgtctctgc agtaagcagt tgtctgaatt ttttaatcgc ctctgttgcg
     1081 atcaagccct gagcgctgat gatcaactgc ggtcagccgt ctgttgtctg ccgtctcctc
     1141 ccttccgtcg tgatacaatc gctgcatgtc gtttgtgccc ctgagagttc attcacagtg
     1201 gagtctgctt gatggcgtcg cgtcaattga tcagatcgtc gatcacgcac aggcacttca
     1261 tctccccgcc attgctttaa cggatacaca tgctttatat ggtgtgcccg attttgtcgc
     1321 ccgctgtcga tcggcaaaga ttcaaccgat cattggcgca gaatttacgg ttgatcgtac
     1381 tcactcgatc gtccttctcg cccaaaatat gatcggctac gccaatctgt ctcgattagt
     1441 gacgcacttg caagcgcggc ccgatcggga agcggctctc gcgcgcgggt tgagtatcaa
     1501 tgatctcaag cagaagaccg atggactgat cgcactatcc ggctattgca acgcggcaac
     1561 tgatgaaccg tatctgcgcg aattaatgga tctcttcgga aacaatcggc tcttcagtga
     1621 attgcagata cttgatcaag atgatgtggt tcaagctcat gcactgcaag ccttagtcga
     1681 tcagttgaat gttcaaacga tcgctacgca tgacatccac tatctatcaa ccgcagatgc
     1741 aaaaaagtac cgggtattga ccgcgatgcg gctgggtcag cgcttcgacg atctctcagc
     1801 ctctacgctc gaagctcgcg accacgtcga agatactcgt tacgatgcga agagtacggg
     1861 cgtttccaac cgcgatgaca cggttagtga gccaattccc ctgcccgatt attcttttcc
     1921 ttcatccgaa gaaatgcaaa gccgctttgc tgagtttcca aacgcgttga acaacaccca
     1981 acgcatcgcc gagctctgtc gcttcgaatt tccgctcggc caataccgtt tccccaaact
     2041 cgatctgccg gacaatcgat cgccgcgcga agagatgtgg tcgctcgccg tcaagggcgc
     2101 ggtcgatcga tataaagtgc ttacgccaga aatcgaagcg cgactcaaga aggaaacaga
     2161 catcatcgat gcgctcggtt attcgccata ctttctcgtc gtcgcggaca tcgtgcgttt
     2221 cgcgcgcgag catcatgtgc ccatctcgcc gcgcggctcg gcctcgtcct ctgtcgtggc
     2281 atattgtctc ggcattcatg atgtcgatcc gattaagcac aatttatatt tcgaaagatt
     2341 cctctcgctc gaacggcacg atccgcccga catcgatatg gatttatgct cgcgccgccg
     2401 cgatgaagtg atcgactacg tgtatcgccg ctatggatcg gatcacgtgg cgatggtctg
     2461 cacctacgcg acgctgcaat cccgattggc gctgcgggag gtttgcaagg tttttgggtt
     2521 gagcgagagc cgcatcaagg aactttccag aaatctgcca agttattgga cgccgcgcgc
     2581 acaacgaaaa gcgcaggaag cgcaattgat tgattccgcg cgcaacgcgg tcgagcgcga
     2641 agcgattgag attagccagc agttgatcgg cacgccgcac catctatcaa ttcatccggg
     2701 cggcatcgtc atcgcccccg atcggattac cgatctcgtc ccactgcaat atgcgacgaa
     2761 gggattattg atcactcaat tcgatctgaa aggaattgag aaactgggct tgatcaagat
     2821 cgatctgctc ggcatcagcg cgttgacggt tgtcgccgat tgtatcgaat tcattcagca
     2881 gcgcaatcca actttctcga tcgaatcgag cccgctcagc gatccggaaa cggcgcgaac
     2941 gctatcggtg gcgaaaacga tcggctgctt ccagatcgaa agtccgggca tgcgcatgac
     3001 cgtgcgcgaa ctaacagccc gcacaatcga tgatctaatc gtcgcgttgg ctttatatcg
     3061 acccggcccg ttgaaaggcg gactgaaaga tgcatttgtg cggcggcact taggcaaaga
     3121 agcagccgag tatctgcatc cgtcgctcga agtaatcttg agcgaaacgt acggcgtgat
     3181 tgtgtatcaa gaacaagtgc tgcgcatcgc acatgagatc ggcggtttca cattgggcca
     3241 agcggatatt ttacgccgcg caatgtcgaa gaagtccgaa cgcgagatgg cacagctgca
     3301 tatggaattt gttgaaggcg cgcaatcgat gagcgggatg accacagcaa ctgccgaaaa
     3361 agtctgggac ttgatgaaag tttttgcggg ttacggtttt ccaaaagcgc acagtgccgg
     3421 ttacggcgcg gtggcctatc gcatggcgta tctcaagaca cattatccgg cggagttcat
     3481 ggccgcgcga ctcgcagtgt ggggtggctt ctattcgccg cgtgtctata tggccgaagc
     3541 acgggaattg ggattgatcg taaagccgcc gcatgtgaat cacagcgacg aagccttcac
     3601 gctcgatccg atcgatcgca ggacgttgtg gatgggactc ggccaagtgc gcgaacttac
     3661 tcatacgacg atcaaatcga tcatgaccca acggcctttc acttcgatcg aacatttttt
     3721 gatccgggcg cagccgcatc acagcgaagt gatcaatttg atcaaggtca atgcatttga
     3781 aggtttaggt gatcaagagg cgctgttatc gcaattcgat cgatcgcatt ggcgaggccg
     3841 tcactccgca caactggatt tgatggaatc aacaactgaa gtcaagcatc ctgagcggag
     3901 cagcgaagcc gctggcttcg cctcgcagtc gaaggacgcg gcaataacct tagaacaacg
     3961 cgccaagtgg gaacaagaaa ttctcggcta ctgggtcgat gtgcatccgg cacaacttgc
     4021 ggcccaacga acgcaaaaga tcgatcgcgt tcgcagcaat gatctcaatc aacatagcgg
     4081 ccaagaagta aatctcatcg gcgtacgctt ggccgagcat cgctttgtcg atcaatcggg
     4141 tgaagcgtgg acgttgatcg atctggaaga tgaagtcggc acgtttcaag ttttgtggaa
     4201 cgaggcaacc tatcggcagt atcgcggctt gctcaagaag cgtgaaccgc ttttgattcg
     4261 cggcaaagtg cggatcgatc gacagggaca gatcgtggtg atggggagcg aggcgaaagc
     4321 aacctagact caatcgccct cgcgaccggt ggcatgacat tgcacacaat cctggaagtt
     4381 ggcgatgcct tctcttaggt gtctattttg aataccaatc ggatcatgct catgacagcc
     4441 atagcaagtg taaaccttga agttagcggg ttgagtgtgg caggttacac aggcgatggt
     4501 gccgccttcg ccgtgattta gcgggaaggt atgcacgaag gttgcacctt gccaggtcgt
     4561 cgtcgtatga cactgcgcgc agttcgtgcc gaactgaccc gcgtgcacag cgggctcagc
     4621 gtgacaggcg acacagcttt gcggcgtgcc tttgaagata ttgttgatgt ggcattgctg
     4681 gcaatggaca ttgatatgcg cgccagttag tttgaacgca gccacgctgt gatcaaattt
     4741 tgcgccctgc cagttgtccg tcgaatggca ctgaccgcag ttggtgccga acgcgccttg
     4801 gtgctgatcg ttcttttgat gacaggtgcc gcagtcctgc ggcgtgcctt tgaagacatt
     4861 gttgacgtgg cattgttgac aggtaacgtt gacgtgcgcg cccgtcagtt tgaacactgc
     4921 caagctgtga tcgaattttg cgccctgcca gctgtcagac gtgtggcatt gcacgcagtc
     4981 cgtaccaaac gcgcccttgt gctgatcgtc tttctggtgg caagcaatac acgtcggtgc
     5041 ggcttgctta tagtcagcga ctgagcgcac attcagatgg cattgacggc aggccacttc
     5101 ggtatgcttg ccgatcagcg tgaaggtcaa tcggttgtga tcgaatttgc tgtagcgatc
     5161 gatcccatcg tgacaggcca tacactcatt accaaaatcg ccaatgtgct gcgtggcgaa
     5221 atcggcttga taactacgat gacaggtttc gcattgagtc cgatcgaact cggccagacg
     5281 ctgaccgtga caatcgatgc aggtgaacgg ctgcccgttc gacagcttca agtgtgtggc
     5341 taacgagaag ccaactcgat cgtgcggaaa gtcagtcgtg ttgaggtgag tcagtgaagc
     5401 ctgggggccg ttgtgctcgg tgtggcaatt gcggcattga tcggcatggg acagcgcgcc
     5461 gtgcagggct tgagcatcat tcagctgctg tccaatatcg gtgtgacagt tcaggcagcg
     5521 cgtcgccatg acctcgctgc tccacggatc gacatgacag gccgcgcaat tgccgccgat
     5581 gtcggcatgt gatcgcacgc cgttcagcgc accttcacct tcgctctgcg cgctgagtgc
     5641 gccgggactg aacatcgatc cgccgctggc gagggcaatg cccgtgacga tcaacagcgt
     5701 caacactcca gcgatgatgc ccagtggtgt gaaacagccg aggcggcgca tagcttactt
     5761 ctcctgccta actcagacga gccggaacca aaatgcaacc acggacacct gcactgcatc
     5821 cgcagcgcgg tgcaagtgtc cgtgtcatcc gtggttaaat tctgctaaat cacttgatga
     5881 aagtcgcgta gtacaacgtc tcgcccacat ggataaaagc cacggtgaat aaggccacgc
     5941 cgatcgggat atggatcgta tgccagactg ccaacaatcg gcgcgcggtc gccagcgatt
     6001 gaatctgccg ctgaacccga tagcgctcgt ccagcaatcg ccgcaattcc gcggcatggg
     6061 cttgacctgt cgcatcgagc tgcttgatcg cccggcggac ttcgcgatca tatttccgct
     6121 gcaaccagcc gcgttccaag accagcgcgg cgctgcccgg cgtttcaacc gaggtggtcg
     6181 atgctaaacg gctcgcgccg agcgcttgta attttgcatc agctactgcg atctgagatt
     6241 ccagatcacg caccgccact tccgcgccat cgaccgtgcg cggcacagcc gtataaatgt
     6301 agcgaccaat gaaaccgctg acgacgatga tcgccgtcaa caggatcaaa gcacccgcca
     6361 gcccgttaaa tttccacgag gtatgcaaca gcaccagata cggaccgacg atgccggtga
     6421 agatatgaat ttgcagccag gtgctcatgc gcccgatcgc gcggccgcgc gcgcgtttgc
     6481 gcaacgaata caaaatctcg gtgctcagca tcatcaagaa gccgaggatg ccgatgccgt
     6541 gtccgaacaa actgctcgag cgcggcagag cttgcgcggc aaccagcgcg taaacaacgg
     6601 tgatggcgat caacgccgcc agcgcgtaat acagttcata cgttttgcgg cgcacggtgt
     6661 tgctcccact gttgatagaa attagtcaga tgggtcagcg cttctgcagc atcatccatc
     6721 atcgcggcgc ggatcgatcg gatgtccacc tgcgccgcga tcaattgata caacggccgc
     6781 gaccaagttt gatcgcccat cacgaatgcg ccgacgatcg attgctcgcc gactagtacg
     6841 cgcacgcgat tgacatcttc acgttccgtc accacccacg catccgacaa catccgccac
     6901 gcttcgctgc cgccacgcga gatggtcacc aaatcttcgt cctgcccgct gccgactgcg
     6961 ccgatgatcg acgtcgacag accggccaac tgcgtgacat tgaaggccac ctcttttgta
     7021 taagccttcg ccgcgcccgc catgtttgct ccagctgcat ggccttgagc gatggctgtt
     7081 gcccacaaca catcgagcac tgcccgacca ctgcgcggat cataggcttg cgcgatatcg
     7141 cccgccgcga aaatatcggg cgcgctggtg cgcatgaatt catcgaccag cacaccccga
     7201 tcgatcttga gctcagctgc tcgggccagc tcgagccggg ggcgcacgcc gatcgctatc
     7261 gccaaaatct ggcagttgat ccgctcgccc gattgggttt ccacaccaat caattttccg
     7321 ttcttgccca gcgcctgctt gatgtgcgta tgcgcgtgaa tgattacgcc ttcggcttgc
     7381 aggcgttcgg cgacgatgcc ggactcggtt tcatccagca cgttcgacca gtatcgatcg
     7441 ccgcgcagaa aataatgaac tcgcacaccg cgctcgcgca gcccctcgac gatttccaac
     7501 gccgtgatgc cgccaccgat aacgacagcg gtgcgaccgc gaccgctcaa cttcagaata
     7561 tgccgcgcgt catccagctt atcgagtttg acgacaccgt tcaactcgcc gccggggaaa
     7621 tcgggcgata cggttgtcga gccggttgcc agcagcaatc gatcgtacgt gacgctctgg
     7681 ccattcgcca acagcacttg atgtgtggcc ggtaatattt tttcagcgcg agatttctgc
     7741 cagcgcaaac ccagcgcttg aagatcgtcg tgactgcgat tgaagagttg cttttcgggg
     7801 atgtcgccgc gcagataata tgccaaaccc ggacgcgaat agaaaccgtg cgtctcttcg
     7861 gcgatcagcg taatgtgtgc cgtggcttcg tgttgacgga tcgcttcggc agcggacaac
     7921 ccggcgatgc ctgaaccgat gatcacgtat tggcgcgcca tcgttaactc tccaccgtga
     7981 acgtgccgat gcgctcgaaa cgcagcacgc tgcgcggaca gcgcagcaca cattggccgc
     8041 aagtcaaaca gtggctgtca ttaatcggca gaccgcgcca cgcatgcgcg cgcacatcga
     8101 tgctgatcgg gcagttatat gaacagatgc cgcactgcac acagcggatc gcctccggca
     8161 caacctgtcc ctcggccagc acttcatggc gcttcagatc gaaatgcaga gaatgactat
     8221 ccgggagcat acaaatatcc gcaggcgaga cgtgaggact tagacatctg tggatcgtaa
     8281 catggcggtt acgtgggttc aactgacaat tgacatgaga aaaaagctac gtttgccact
     8341 gagcaacaag cttggacccg cctaccgcaa tcaacgcccg atgacaagtt aagcacgatg
     8401 cgtttctatg atttcggtat tgatcgatag gggacagatc gtggtgatgg gcatcaggcg
     8461 aaggcaatca cgctaacgta gtcgaggcac ctccatgcct cgattgggac ggcataggga
     8521 tgccgttcct gcgaaaattc attcacggca aagtgcggat cgatcggcag gcacagattg
     8581 tagtgatggg cagtgaggcg aaagccattt aaatgagtag gttatttttc tatcaccacg
     8641 cccgccatca acaaacctaa ctgctccggc gtggcggcac cccgatcgac gatgccgacg
     8701 atacggcctt cgtacatcac cgcaatccga tcggaaattt cttgaatctc atcgaggtct
     8761 tcggagatca acaggatcgc agttccggca tcgcgctgct ccagcaagcg gtggtggatg
     8821 tattcggtcg cgccgatgtc cacaccgcgc gtcggctggg ccgcgatcaa gaccttgggt
     8881 tcacgcgaga gttcgcgcgc cagaatcagt ttctggatgt tgccgcccga taaattcttc
     8941 agcggtgttt ccaaacgagg agttttgacg ttaaagcgat cgaccagttc acgcgcgcgt
     9001 gatgccatcc gcccgaagtc cataaaaatg ccgtgtgtat acttgggtga aacatgatcg
     9061 tgcagaaaca aattctcttg gacggtaaat tcacgaatcg ctccatcgcg catgcgctct
     9121 tcgggaatgt aggcttgccc aatcgccatg cgttcgtgca gtgatgcgtt agtcgcatcc
     9181 tgctcattca gaacgatcga accagaggta tgcttgcgca atccggcgag gcattcagcc
     9241 aattcgcgct gtccattgcc cgatactccg gcgaggccga cgatctcacc ggcgcgaact
     9301 tccaagctga taccacgtaa catctcggtg ccgttatcgc cgcgggcgtg cagcgcatcg
     9361 acgcgcagcc gcacttcgcc cgcgtgcagt gcttgcggtg cgagcggctt cacttctcgc
     9421 ccgaccatca tctgcgccag ctcgttgcgc gtgacacctt tcgtcggccg cgtgccgacg
     9481 acccgcccat cgcgcagcgc cgtgactcga tcgctgatcg ccgtgacttc gtgcagtttg
     9541 tgcgagatga aaatcaaagc gtggccttca ctcgccatcc gccgcagcgt gacaaataga
     9601 tcgtccactt cctgcggcgt caataccgcg gtcggctcgt cgagaatgat caaactcgcg
     9661 ccgcgataca gcgctttcac tatttcgacc cgctgttgtt cgcccacagc caactgccac
     9721 accggaactt tcggatcgac tttcaacccg taagcttgag ccaacgatcg gatgcgctcc
     9781 gaaacccgat caagatcaag tctcggctcg cgtgatgatc tcaagcctaa cgccacgttc
     9841 tcggctacgg tcaacgtcgg caccagcatg aagtgttgat ggatcatgcc gatcccggcg
     9901 cggatcgcat ccgccggtga tcgaaattcg atcggctgtc catcgatcag gatctgacct
     9961 tcatcgggct ggtacaagcc gtagagctgg cgcatcaacg tgctcttgcc tgcgccgttt
    10021 tcgccgagca gcgcatgcac ttcaccggcc gtcacatcga agtcgacgtg atcgttagct
    10081 aacacgccgg ggaaacgctt gacgatgccg cgcatctcaa gctgttggat tcgtcgtccg
    10141 gaaatggatt gcgaattagc ggccatgtta aacaatctga aataggggcg agatgacctc
    10201 tcgcccctat
//
LOCUS       DASRTJ010000272         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_157988,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000272 DASRTJ010000000
VERSION     DASRTJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157988"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1587
                     /gene="lysA"
                     /locus_tag="VFF70_05610"
     CDS             349..1587
                     /gene="lysA"
                     /locus_tag="VFF70_05610"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZY44213.1"
                     /translation="MTGFVFHDQQWWCDAAPIDLLADRHGTPLYVYSRARIIESYNRV
                     ATAFKPLNAHLHYSVKANSSGAILRVLHDLGAGFDVVSGGELFRVLKIGADPASIVFA
                     GVGKTRAELEYALKNKVGWINVESVQELEVLNDLAGDKKLETSIALRVNPSIEAETHE
                     HIATGGHRSKFGLDLDEARSVLAQSDRFPRLNVTGLHMHIGSQLATADRTVEALKKLL
                     DLAQDHSIQMLDIGGGFPVRYHPTDDYPDPIEFANMIFHTLASPLASRPLQLIIEPGR
                     SIVADAGALIAEVQYVKQRESRRIIVINASMTELIRPALYDAYHHIVAAQPRDDTHAL
                     ADIVGPVCESADVLGIDREFPEIRRGDRVMIMTTGAYGSSMASNYNSRPRPAEVMVDG
                     AKSHLIRRRETWNDLVALEE"
     gene            1705..2592
                     /locus_tag="VFF70_05615"
     CDS             1705..2592
                     /locus_tag="VFF70_05615"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZY44214.1"
                     /translation="MIKSEWYTYAEVFPNMLHSRLFDAPAVYTWIFTPSIVIGLVSLS
                     VLYAYLVYADRRDGYWGKVLKARHIAFFTAGILTLYLALQSPLDPLSDYALFSAHMTQ
                     HLLLALIAPAFLLFGVSMRWLRALYQIPIIGPLLAIFTNPFVALIVFNFNLWLWHVPA
                     LYEGALRDENIHVIQHLLFIVTGTLMWLPVLHTVPPNRPMGYLAKIGYLFFGMLSSSI
                     LGAIFTFAGDVIFKFYGDIPLNFGLTPLDDQQLAGSIMWVPGGGIFFTSLLITFAAWL
                     QSEDRKGQALDQQQKEVTQ"
     gene            complement(2637..>2813)
                     /locus_tag="VFF70_05620"
     CDS             complement(2637..>2813)
                     /locus_tag="VFF70_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZY44215.1"
                     /translation="LLNDHFDFRPGAIIHNLNLRRPIYKATAAYGHFGRHDIDAPWEN
                     MDKAAILRKEAGLA"
BASE COUNT          649 a          753 c          718 g          693 t
ORIGIN      
        1 cgcactttgc acttccattg gcatatccga catcggcgcc attgctaagt cgtgatcaga
       61 ggtcaatgcc gcgctcgacc cacaacccgc tgcggttcca ctgagaaccg ccaagagaat
      121 gagaaacgac aaccgccgat atatcttatg catattagcc ctccatatat atgaacatat
      181 tctacaggtg gatgttctga aaaattagtg atggctgtca tgcgttttag cgggaactgt
      241 catttaaaag attaagcaga ggttaaaaca gtcgacgccc aatgtaattt cacacatatt
      301 ctggtagact aaagcagaat gatgtatccg attcgagagg ggtttcacat gacaggcttt
      361 gtttttcacg atcaacaatg gtggtgcgac gcagccccga tcgatcttct ggccgatcgg
      421 catggcacgc cgttgtacgt ctacagccgc gctcggatca tcgaaagcta caaccgagtc
      481 gcgactgcat tcaagccgct caacgctcac ctccactatt cagtcaaagc gaacagtagc
      541 ggcgcgattc tgcgcgtgct gcacgatctc ggtgcgggct tcgatgtggt gtccggcggc
      601 gaattgtttc gcgtgctgaa gatcggcgca gacccggcct cgatcgtttt tgcgggtgtg
      661 ggcaagactc gcgccgagtt agagtacgcc ttgaaaaata aagtgggctg gatcaacgtg
      721 gagtccgtgc aggaactgga agtgctgaat gatctcgcgg gcgataaaaa acttgaaaca
      781 tcgatcgcgc tgcgggtgaa tccatcgatc gaggcagaga cacatgagca cattgcgaca
      841 ggcgggcatc gatcgaaatt cgggctcgat ctggacgaag cccgatcggt cttagctcaa
      901 tccgatcgat tcccgcgcct caacgtgacc gggctgcata tgcacatcgg ctcacaactg
      961 gcgacggctg atcgaactgt ggaggcactg aaaaagttac tcgatcttgc gcaagatcat
     1021 tctattcaaa tgctcgatat cggtggcggt tttccggttc gataccatcc caccgacgat
     1081 tatcccgatc caattgaatt tgcgaacatg atttttcata cgttagcctc gcccctcgcc
     1141 tcccgcccct tacaattgat cattgaaccc ggccgatcga tcgtcgccga tgcaggcgcg
     1201 ctcatcgccg aagtgcaata cgtcaaacaa cgtgagagtc gccgcatcat tgtgatcaat
     1261 gccagcatga ccgaactcat tcgaccggca ttatatgacg cgtatcatca cattgttgct
     1321 gcacagccac gcgatgatac gcatgcattg gccgacatcg ttgggccggt gtgcgaatcc
     1381 gctgacgtgc tggggatcga tcgagagttc ccagaaatcc gacgcggcga tcgagtaatg
     1441 atcatgacga caggcgctta cggatcgagc atggcgtcta actacaacag tcggccacgt
     1501 ccggcggagg tgatggtaga tggagcaaaa tcccatttga ttcgccgccg cgaaacatgg
     1561 aacgatctag ttgcactgga agaataatgg ctctcattac acatcgccag tgcgagccgc
     1621 gtaacagcaa agcaatctcg gcgtcgatct agggattgct tcgtcggcct aacggcctcc
     1681 tcgcaatgac gctgcgtaac gtgaatgatc aaatcagaat ggtatactta cgccgaggtt
     1741 tttccgaaca tgcttcactc aagattattt gatgcaccgg cagtctacac ttggatcttc
     1801 acaccgagca tcgtcatcgg tttagtgagc ctaagcgtat tgtatgcgta tctcgtctat
     1861 gccgatcggc gtgatggata ttggggcaaa gttttgaaag cgcggcacat cgctttcttt
     1921 accgccggaa tcctcacact ctacttggcc ttgcaatcac cgctggatcc gttgagcgat
     1981 tatgcgttgt tctcggcgca catgacccag catctattgc tggcgttgat tgcgccggca
     2041 ttcctgctct tcggtgtgtc catgcgttgg ctgcgtgcac tctatcaaat tccgatcatc
     2101 ggtccgctgc tggctatctt taccaatccg ttcgtcgcgc taattgtctt caacttcaat
     2161 ctttggcttt ggcatgtacc ggctctgtat gaaggcgcac tgcgcgacga aaacattcac
     2221 gtcattcagc acttgctgtt cattgtgacg ggcacactca tgtggctgcc cgtgctgcat
     2281 acagttccgc ccaatcgtcc gatgggttat ctcgccaaaa tcggctactt gtttttcggc
     2341 atgttatcca gcagcatcct cggggcgatc tttactttcg ctggagacgt gatcttcaaa
     2401 ttctacggag acattccttt gaactttggt ctaacgcctc tagatgatca acagctggcc
     2461 ggatcaatca tgtgggtgcc gggcggcggt atctttttca catcgctgct catcacgttt
     2521 gcggcctggc tccagagcga agatcgcaaa ggccaagcac tggatcaaca acaaaaagag
     2581 gtgactcagt gagtcacctc tcttattatt gtatggcaag ttgccaatcg atccgactac
     2641 gctaaacccg cttctttacg caagatagct gccttgtcca tattttccca cggcgcatcg
     2701 atgtcatgcc ggccgaagtg tccgtaggcc gccgtcgctt tgtagatcgg tcggcgcaag
     2761 ttgaggttat gaataatcgc gccgggccgg aagtcaaagt gatcgttcaa caa
//
LOCUS       DASRTJ010000273         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_271723,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000273 DASRTJ010000000
VERSION     DASRTJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271723"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFF70_05625"
     CDS             <1..952
                     /locus_tag="VFF70_05625"
                     /inference="COORDINATES: protein motif:HMM:NF027230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HZY44216.1"
                     /translation="YSDDFGKTWTQTKVAAALLQPSTSGRPLGSPEDFTHAEEIAKEP
                     ERVIKTWNIQPGRASEPNVLYAGVQHAALFKSTDRGETWELNEALYNHPHRGQFNPGA
                     GGLCLHTIILDPNDGNRMYVAISAGGFYRTDDGGQTWLARNKNLLADFSPVKYPEFGQ
                     CVHKVDMHPARPNVLYQQNHCGVYRSDDFGDNWIDTGLDRLPSRFGFPVVAHPHDPKT
                     VYHVLEDSQDYRLSVDGKFSVWRSKNEGNTWKRLSNGLPQNAYLTILRDAMAADTVDD
                     AGLYVGSNTGQLFYSRDEGDSWEVLADFLPPIQSVETAVI"
     gene            957..1328
                     /locus_tag="VFF70_05630"
     CDS             957..1328
                     /locus_tag="VFF70_05630"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HZY44217.1"
                     /translation="MPTSDSVKFHFAGKSPIVQRIYDRLLTILRKFGPVIEEPKKTSI
                     HLVNVSALAGVEVRQNYLLLNIKTDYPIDSPRVERAEKISARRYHQRVRLTALSDLDT
                     ELKQWLKDAYALSGAKSKSID"
     gene            1393..>1707
                     /locus_tag="VFF70_05635"
     CDS             1393..>1707
                     /locus_tag="VFF70_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZY44218.1"
                     /translation="MADNGSKENFDLEIPEGMTYYYLAILKKDPAWTADESAELDQLQ
                     AAHVANYRRLAEEGKLIVSGPLIDNAFIRGIDVFRTQSLEEAKRLSETDPMALSQRLE
                     YEF"
BASE COUNT          436 a          432 c          452 g          387 t
ORIGIN      
        1 ctattcagat gactttggca aaacgtggac acagactaaa gtagcagccg ctctgctgca
       61 gccttctacc tctggccggc cacttggttc acctgaagat tttactcacg ccgaagaaat
      121 cgccaaagaa ccagagcgtg tcattaaaac ttggaacatt caaccgggcc gcgccagcga
      181 accgaatgtg ctatacgccg gagttcagca cgccgccttg ttcaagtcta ccgatcgagg
      241 cgaaacgtgg gaattgaatg aagcgcttta caatcatccg catcgcggcc aattcaatcc
      301 cggcgcaggc ggtttgtgtc tgcataccat catccttgat cccaatgatg gtaatcgcat
      361 gtacgttgcc atttcggcgg gcggtttcta tcgaacggat gacggcgggc aaacctggct
      421 ggcgcgcaac aaaaatttgc tggcagattt ttcgccggta aaatatccgg agttcggcca
      481 gtgtgttcac aaagtcgata tgcacccggc gcgaccgaat gtgctgtacc aacaaaatca
      541 ctgcggcgtc tatcgatcgg atgactttgg cgacaattgg attgacactg gtctcgatcg
      601 gttgcccagc cgatttggtt tccccgttgt ggcgcatccg catgatccca agacggtata
      661 tcatgtgctg gaagacagcc aagattatcg attgagtgtc gatggcaaat tctccgtctg
      721 gcgcagcaag aacgaaggca atacatggaa acgcttgtcg aacggcctgc cgcagaatgc
      781 gtacctcacc attcttcgcg acgccatggc cgccgacacg gtcgatgacg cgggcttgta
      841 tgttggctct aacaccgggc aacttttcta cagccgcgac gaaggcgatt cgtgggaagt
      901 gctggcggat ttcctgccgc cgatccaatc cgttgagacg gcggtgatct agaacaatgc
      961 caacctccga ctccgtcaaa tttcacttcg ctggaaaatc gccgatcgtt cagaggattt
     1021 atgatcgatt actgacgata ttgcgaaagt tcgggccggt tattgaagaa ccgaagaaaa
     1081 catcgatcca cttggtgaat gtatcggcct tggccggtgt tgaagtgcgc cagaactatc
     1141 tgttgctcaa catcaaaacc gattatccga tcgatagtcc gcgtgtcgag cgggctgaga
     1201 agatttctgc gcggcgatat catcagcgag tcaggcttac tgcactaagc gatcttgata
     1261 ccgaacttaa gcaatggctc aaggatgctt acgcgctcag tggcgctaag agcaaatcga
     1321 tcgattagga acaacaaaaa cttccgaagc agttacaaag cttcggaagt ttatttttgg
     1381 agaatgcaga ccatggccga taacggaagc aaagaaaatt tcgatttgga aattccggaa
     1441 ggcatgacgt attattatct ggcgatcttg aagaaggacc ccgcgtggac ggctgacgaa
     1501 tcagccgaac tcgatcaatt gcaagcagcg cacgtcgcca attatcggcg tctggcagaa
     1561 gaaggcaaac tcattgtcag cggaccattg atcgacaacg ccttcatccg aggcatcgat
     1621 gtgtttcgga cgcaatcgct tgaagaggcg aagcgtctga gtgagaccga tccgatggca
     1681 ctgtcgcagc ggctggaata tgaattt
//
LOCUS       DASRTJ010000274         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_242783,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000274 DASRTJ010000000
VERSION     DASRTJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242783"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFF70_05640"
     CDS             complement(<1..189)
                     /locus_tag="VFF70_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44219.1"
                     /translation="MKKKILLLDGHSLAYRAFYGLPLYDQSGRPRFSTKRGEFTNAIY
                     GFASMMFKVWSDEQPDYIA"
     gene            complement(372..1553)
                     /locus_tag="VFF70_05645"
     CDS             complement(372..1553)
                     /locus_tag="VFF70_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY44220.1"
                     /translation="MTTLRRPSFFFRLLILSIVILIGCSSNPIAPAAPTINAAAPILP
                     TRTIEPVPVTPTPAPTIAPTREAATSYYSDGTHAYATGHYTSALLSFTQAAQLDPNNA
                     LIYMSRAAVYQGLIDFDGAIADYTQAIQLDPKNVTAYVNRGEVYALRKQFDLALKDMD
                     YALVLTPNEPSVYVNRGRVIQSSGIITAAIDNYSHALQIDPNYAPAYFYRALAYINTR
                     RPDQQTIDLRAINDLSAVIGLQPDNIDAYLARAAAYVAGGDLGHALTDYTQALAIDPE
                     NIEALIQRGHLHDTRREFTAALQDLNQALLLEPQSFDAFYNRGLTYLDQNQTDRAIND
                     FTAAIAIDASKWRAFLQRGLSYENKNNRTSALANLKAALALTTNPTDKAIVQAEMDRL
                     K"
     gene            1612..>2749
                     /locus_tag="VFF70_05650"
     CDS             1612..>2749
                     /locus_tag="VFF70_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZY44221.1"
                     /translation="MTSFKDNLNERRFSAELEALHEATMMITAELSLDRVLQQITDLA
                     RGLAGATYAALGVPDQAGGLEEFIVSGIDEATVKHMGHLPIGRGLLGLLLREPQPIRI
                     PRLQDDPRFSGFPEYHPLMISFLGVPIISKGKLLGNLYLTNKIGAEEFSDQDERLIAM
                     LAAQAGIAIDNANLYARVQRLAVLEERDRIAMDLHDGIIQSIYAVGLTLEYVDLLMEE
                     QPPAAHERLKQSIGGLNEVIRDIRNYILDLRPQRFKSKNLIAGLNDLVRAFKANTFIA
                     TEVQSSDRADADLTPDQSTGLFHITQEALANIAKHAKARSVIISLQRDPLAISLGVQD
                     DGRGFDLPKVQSYTGHGLENMQERAKALGAEFRVMSEVGKGTKIE"
BASE COUNT          616 a          655 c          829 g          649 t
ORIGIN      
        1 cgcgatatag tccggctgct cgtcgctcca tactttgaac atcatggagg cgaagccata
       61 gatcgcgttc gtgaactcgc ctcgcttcgt cgagaaacgc ggacggccac tttgatcata
      121 aagtggtagt ccatagaaag cgcggtaggc aagtgagtgg ccgtcgagga gcaagatttt
      181 ctttttcatg gttgctcgaa tctttgattt ctaatttaac atttgcacgt cattgcgcgg
      241 cgcttctttg ggcaccgaag cgatctcagc tgattatccg ggagattgct tcgccacccg
      301 taggctttgc ctgttaggtg ctgagttgcg gctcgcaatg accgatcaat cacgtatcac
      361 gtcatcgtgc atcacttcaa ccgatccatc tccgcttgca cgatcgcttt atcggttgga
      421 tttgtcgtca aggctagcgc ggctttcaaa ttcgccaggg ccgaggtgcg attatttttg
      481 ttttcgtaag agaggccgcg ctgtaagaat gcgcgccatt tgctggcatc gatcgcaatg
      541 gcagccgtga agtcattgat cgcgcgatcg gtctgattct gatcgaggta ggtcaggccg
      601 cgattgtaaa aggcatcaaa cgattgcggc tcaagcagta aggcttgatt caaatcttgg
      661 agagccgccg taaattcacg gcgagtgtca tgcaaatgcc cgcgttgaat cagcgcctca
      721 atgttttccg gatcgatcgc gagcgcttgc gtgtaatcgg tcaaggcatg accgagatcg
      781 ccgcccgcaa cataggccgc ggcgcgggcg aggtaggcat caatgttgtc cggctgtaag
      841 ccgatgacgg cgctgagatc attgatggcg cgcagatcga tcgtctgctg atcgggccgg
      901 cgggtgttga tgtacgccag cgcgcgataa aaataagctg gagcatagtt tggatcgatc
      961 tgcagggcgt gcgaataatt gtcgatggcg gccgtgatga tgcccgaaga ttgaatcacg
     1021 cggccgcgat tcacatacac cgatggttca tttggcgtga gaactaaggc atagtccatg
     1081 tcttttaagg cgagatcgaa ttgtttgcgc agggcataca cttcgccgcg attgacgtag
     1141 gctgtcacgt tcttgggatc gagttgtatg gcttgcgtgt aatcggcgat ggcgccgtca
     1201 aaatcaatca ggccttgata cactgcggcg cggctcatat agatcagcgc attgttcgga
     1261 tcgagttggg cggcttgggt gaaactcagc agggctgaag tgtaatgacc ggtagcatac
     1321 gcgtgtgtgc cgtccgaata gtaacttgtc gcggcttcac gtgtcggcgc aatggttggc
     1381 gctggggtcg gcgtgaccgg caccggttcg atcgtgcgtg tgggcaagat cggcgcagcg
     1441 gcattgatcg tcggggccgc aggagcgatc ggattagacg agcaaccgat caagatcacg
     1501 atcgataaaa tcaataatcg aaagaaaaaa cttggtctgc ggagtgtggt catgatcgaa
     1561 ttgtaacacg agtcgcgcat gttataatct acgcggacaa ggacagccag catgacttct
     1621 ttcaaagaca atctgaatga acgccgtttc agcgcggaat tggaagcgct gcacgaagcg
     1681 acgatgatga ttacggccga gctgtcgctc gatcgcgtgc tgcaacagat taccgatctg
     1741 gcacgcgggt tggcgggcgc gacgtatgcg gcacttggtg tgcccgatca agcgggcggg
     1801 cttgaagagt ttattgtatc cggtatcgat gaggccacgg tcaagcacat ggggcatctg
     1861 ccgatcggcc gcggactgtt gggtttgctg ctgcgtgaac cccagccgat tcgcatcccg
     1921 agattgcagg acgatccgcg ctttagtggt tttcccgaat atcatccgct gatgatcagt
     1981 tttctgggcg tgccgattat ttccaaaggc aagctgttag gcaatttata tttgacaaac
     2041 aagatcgggg ctgaagagtt ttccgatcaa gatgagcgct tgatcgcgat gttggccgcg
     2101 caggccggca tcgcgattga caatgcgaat ttgtatgcgc gggttcagcg cttagcagtg
     2161 ttggaagagc gcgatcgaat tgcgatggat ctgcatgacg gcatcattca atcgatctac
     2221 gcagtgggct tgacgctgga gtatgtcgat ttgttgatgg aagaacagcc gccggccgcg
     2281 cacgagcggc tcaagcaatc gatcggcggt ttgaatgaag tcatccgcga tatccgcaac
     2341 tacatcctcg atctacgacc gcaacgcttt aagagcaaga atttgatcgc ggggttgaat
     2401 gacctcgtgc gggctttcaa agcgaatacc tttatcgcca cggaagtaca atcctccgat
     2461 cgagcggatg ccgatttgac gcccgatcaa tcgaccggcc tgtttcatat tacacaggag
     2521 gcgctggcga atatcgccaa gcatgccaaa gcccgcagcg tgatcattag tttgcagcgt
     2581 gatccactgg cgatctcctt aggggtgcag gatgatggcc gtgggttcga tctaccgaaa
     2641 gtgcagtcgt atacaggtca cggcttggaa aatatgcaag agcgagccaa ggctttgggc
     2701 gcggagttta gggtaatgag tgaggtaggc aaaggcacga agattgaag
//
LOCUS       DASRTJ010000275         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_120197,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000275 DASRTJ010000000
VERSION     DASRTJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120197"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFF70_05655"
     CDS             complement(<1..327)
                     /locus_tag="VFF70_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZY44222.1"
                     /translation="MNSTFPFLTLITFIPAVTGAIILLMPKERGTLIKVTAAVATLIS
                     AVLAFTIFITYDKTVGGYQFIEGPYPWVSSLGINYLVGADGINLVMLILTGIVSFMGV
                     LISWGIN"
     gene            complement(361..1911)
                     /locus_tag="VFF70_05660"
     CDS             complement(361..1911)
                     /locus_tag="VFF70_05660"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZY44223.1"
                     /translation="MEFINNNLLTLIVFSPAIFAVLMLIVPANDRGLKYAALSFSLIT
                     TALTIYLWIQYPAATADSSGFKFVPNIVPWLAQINSNYHVGVDGISLAMILLTGILTP
                     LGVLISFRITDRVRVYLALFLLLETGMLGVFASLDLIIFFIFWEIGLVPMYFLINVWG
                     SKNRQYASFKFFIYTMAGSVGMLLAIQVIWFALGHTYNMNEIAAALPNFNGTLFGLPA
                     QTVKGIAFWAFTIAFAIKVPIWPFHTWLPDAHTEAPTAGSMLLAGVLLKLGAYGFLRI
                     VLPFFPDQAAQFAVVLAVLALAGIVFTALAAFGQNDFKRLVAYSSVNHMGFVVLGIAV
                     AAASYGSTDPAVKASAVIAVNGAVLQMFAHGLSAAAMFALVGVVYDRAHTRDLNELGG
                     LWAVMPIYGGILIFSAMANLGLPGLAGFPAEFQIVRGAWPVFTLITALSMIGLFLTGA
                     YVLKAIQKVLHGPVNHELLHHAEMTEISWTERLALAPLMALMLAIGLYPFWLMQPIND
                     AVTKLIGG"
     gene            complement(2001..>4239)
                     /locus_tag="VFF70_05665"
     CDS             complement(2001..>4239)
                     /locus_tag="VFF70_05665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZY44224.1"
                     /translation="HQTLVSFVWLIPAMPLISFFAILLFTRGKNRLSHTIAIGAMAIS
                     FILAQIVFWNVVSNGEELAANPIAQSFPWLPAGATSFNMGVLVDPLTAIMLFMVPLAC
                     LMIFIYSVGYSNFNKTRDLRDEPGKQPEHGVEPLYSRFFAFLSLFAAGMLTLVVSDNL
                     LMLFIGWEIMGLCSYLLIGFWYARNYSDPKKITPRGAAVKAFMTTRVGDVFMMLGIVF
                     LYSATGSLSFREVFSAVSTHALATALFIGIPAAQVIFGLLFIGTVGKSAQFPLHVWLP
                     DAMEGPTPVSAMIHAAAMVSAGVYMVIRMFPLLAGTATEGAPTFGVMALIGGFTALFA
                     ATIALAQNDIKKVLAYSTISQLGYMIAALGIGAWVAAAFHLITHAFFKALLFMGSGSV
                     IHGVEHGQHALHETHDDHALTTDASAHAEHGHAEHTGPEFDPQDMFNMGGLRKKMPWT
                     FWTFLIGGFALSGFPLITAGFWSKDEILGEAYAHNPIIFWTLALAALLTAFYTARQIS
                     LSFLGKARTEAAEHAHESHWTMILPLVILSFFAIFAGFAGTKGDFPFIGLALRPPLVH
                     SSWFESFLEPAITQTGIQLRAASFTFAPFIVSVIVALGGLFLGWLVYGRKPLAAGAPD
                     PLAKLGGVWTLLKNKYYIDELYAAVFVQPVTRFAAWMGDVFDRGVIDGVLHGIGRGAV
                     SVATAFRSFDTSAINGGADALANDVKRFGRWLREIQTGRVQNYLLFVLVSVVLLAALY
                     ITLFS"
BASE COUNT         1151 a         1215 c         1098 g          775 t
ORIGIN      
        1 gttgatgccc cacgagatca acacacccat gaaactgacg atgccggtga ggatcagcat
       61 cactaaattg atgccgtccg cgccgacgag gtaattgata cccagggagg atacccacgg
      121 atacggccct tcgatgaatt gatagccgcc gaccgtttta tcgtacgtga tgaagatggt
      181 gaacgctaac acggccgata tgagagtggc caccgccgcc gtcactttga tcagcgtgcc
      241 gcgctctttc ggcatcaaca aaatgatcgc gccggttacc gccgggataa atgtaataag
      301 agtcaaaaat ggaaaagttg aattcatatc aaagcctcaa aagacttttc ggtataccag
      361 ctacccgccg atcaattttg taaccgcatc attgatcggc tgcatcagcc agaacggata
      421 cagaccgatc gccaacatca acgccatcaa gggcgccaaa gccagccgct cagtccaact
      481 gatctcggtc atctcggcat gatgcaacaa ctcatgattc actggtccat gcaacacttt
      541 ctgaatcgcc ttcagcacat acgcgcccgt cagaaacaag ccgatcatcg acagcgcggt
      601 gatcaacgtg aagaccggcc acgcacctcg cacgatttgg aactcagccg gaaaaccggc
      661 cagtccgggc aagccgagat tcgccatcgc gctaaagatc aagatgccgc catagatcgg
      721 catgacggcc cataatccgc cgagctcatt cagatcgcgg gtgtgcgctc gatcgtagac
      781 cacgccaacg agagcgaaca tcgcggcagc ggacaagcca tgcgcgaaca tttgcaggac
      841 tgcgccgttc acagcgatca ccgcactcgc tttcacagcc ggatcggtgc tgccatacga
      901 agctgccgcg accgcaatac ccaataccac aaagcccata tgattgaccg acgaataagc
      961 gactaagcgt ttgaaatcat tttgtccaaa ggcggccaat gccgtgaaga caatgcccgc
     1021 taaagccaac acagccaaca cgactgcgaa ctgcgcggct tgatcgggga agaacggtaa
     1081 tacgatacgc aagaaaccat acgcgcccaa cttcaacaac acaccagcga gcagcatcga
     1141 tccggcagtt ggggcttcag tgtgcgcatc gggcagccac gtgtggaacg gccagatggg
     1201 gactttgatc gcaaacgcga tcgtaaacgc ccagaatgcg atccctttga cagtttgcgc
     1261 aggcaatcca aacaatgtgc cgttgaaatt cggcagcgcc gcagcgatct cgttcatgtt
     1321 gtacgtatga cccagcgcga accagatcac ttgaatcgcc aatagcatgc cgaccgaacc
     1381 ggccattgta tagatgaaga atttgaacga ggcatactgt cgattcttgc tgccccacac
     1441 attgatcagg aagtacatcg gcaccagacc gatttcccag aaaatgaaga agatgatcag
     1501 atcgagcgag gcgaacacgc ccagcatgcc ggtctccagc agaagaaaca gcgccaaata
     1561 gacgcgcact cgatcggtga tgcggaacga aatcaaaacc ccgagcggtg tcaggatacc
     1621 ggtcagcagg atcatcgcca ggctgatgcc atcgactccg acgtgatagt tgctgttgat
     1681 ttgcgcgagc catggcacga tgttaggaac aaacttgaaa cccgatgagt cagctgtggc
     1741 cgccggatat tgaatccaca aatagatcgt caacgccgtc gtgatcaagc taaagctcaa
     1801 ggccgcgtat ttcaagccgc gatcattggc gggaacaatc aacatcagca ccgcaaagat
     1861 ggccggcgaa aagacgatca aagttaaaag gttgttgtta atgaattcca tgtgcaatcc
     1921 tcaaatactg gatgctctcg ccgccgtcct cggcagcgca tcacaaccac ttccgcgccg
     1981 agacgacgcg gggagcatcg ttacgaaaag agtgtgatat acaacgccgc caataaaacc
     2041 acgctgacta ggacaaataa caaatagttt tgaacgcggc cggtttgaat ctcgcgcagc
     2101 cagcggccga aacgcttcac gtcattcgct aaagcatctg ctccgccatt gatcgccgaa
     2161 gtgtcaaacg atcggaaagc tgtcgccaca ctcactgcgc cgcggccaat gccatgcagc
     2221 acaccgtcga tcacgccgcg atcgaacacg tcgcccatcc acgccgcgaa gcgcgtgacc
     2281 ggctgtacga aaactgcggc gtagagttca tcgatgtaat atttgttctt caacaatgtc
     2341 cacacaccgc cgagttttgc taatggatca ggcgcacccg cggctaatgg cttgcggcca
     2401 tagaccagcc aacccaagaa caatccacct aacgcaacga tcactgaaac gatgaacggt
     2461 gcgaaggtaa acgacgcagc tctaagttga atgcccgttt gagtaatcgc cggctcaaga
     2521 aacgactcaa accatgagct atgaaccagc ggaggccgaa gcgctaaacc gataaacggg
     2581 aagtcgcctt ttgtgccagc gaagccggca aagatggcaa agaaactcaa gatgaccagc
     2641 ggcaggatca tcgtccaatg tgattcatgc gcatgctcag ccgcttccgt gcgcgctttg
     2701 cccaagaacg ataagctgat ctgccgcgcc gtgtagaacg cggtcaacag cgcggcaagc
     2761 gctaatgtcc agaagataat cggattgtgc gcgtaagctt cacctaagat ttcatccttc
     2821 gaccagaaac cggcggtgat caacgggaag ccgctcaacg caaaaccgcc gatcaagaat
     2881 gtccagaaag tccacggcat cttcttgcgc agaccaccca tgttgaacat gtcctgtgga
     2941 tcgaactccg ggccggtatg ttctgcgtgg ccgtgttccg cgtgagcaga tgcgtctgta
     3001 gtcagtgcat gatcatcatg cgtttcatgc agcgcatgct gtccgtgctc gacaccgtga
     3061 atcaccgagc cggagcccat gaataacaag gccttgaaga acgcatgcgt aataagatgg
     3121 aatgcagccg cgacccacgc gccgataccc aacgcggcaa tcatgtaacc cagctgtgaa
     3181 atcgtcgagt aggctaaaac ttttttgatg tcattctgcg ccaaggcgat cgtcgccgca
     3241 aacaatgcgg tgaagccgcc gatcaatgcc atcacgccaa atgtcggtgc gccctcagtc
     3301 gccgtgcccg cgagcaaggg gaacattcga atgaccatgt acacacccgc gctgaccatt
     3361 gcggcggcgt gaatcatggc gctgaccggc gtcgggccct ccatcgcatc cggcagccag
     3421 acgtgcaacg ggaactgcgc gctcttgccg accgtgccga tgaacaacag accgaagatt
     3481 acctgcgcgg ccggaatgcc aatgaacaag gcggtcgcca acgcatgtgt actcaccgca
     3541 ctaaaaactt cgcggaagga caatgagccg gtcgctgaat agaggaacac gatgccgagc
     3601 atcatgaaca catcgccgac gcgcgtggtc atgaaggctt taaccgccgc accgcgtgga
     3661 gtgatcttct tgggatcgct atagttgcgc gcgtaccaga aaccgattag caggtacgag
     3721 cacaagccca tgatttccca gccgatgaac aacatcaaca ggttgtctga cacgactaga
     3781 gttaacatac ccgcggcgaa caatgacaag aatgcgaaga agcgcgagta tagcggttcg
     3841 acaccgtgct ccggctgctt gcccggttca tcgcgtagat cgcgcgtctt gttgaaattg
     3901 ctgtagccga cgctgtagat aaagatcatc aaacaggcca gcggcaccat gaacaacatg
     3961 atcgccgtca gcggatcgac cagcacgccc atgttaaacg acgttgcacc ggcgggtaac
     4021 cacgggaagg attgagcgat cgggttagcg gctaactctt cgccattact caccacattc
     4081 cagaacacga tctgcgcgag gatgaacgag atcgccatcg ccccgatcgc gatggtgtgg
     4141 ctcaagcgat tcttgccgcg ggtaaacagc aagattgcaa agaatgatat taacggcatt
     4201 gccgggatca gccagacgaa ggaaactaaa gtttgatgg
//
LOCUS       DASRTJ010000276         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_91236,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000276 DASRTJ010000000
VERSION     DASRTJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91236"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFF70_05670"
     CDS             <1..338
                     /locus_tag="VFF70_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44225.1"
                     /translation="CTLTSPAPIFDLLSDHSLAVLLAEEIEMLLAERRAAWVAQPNLF
                     DERLARTGPWLLFAACLKALWVRYAHYSVSIDDPDYHLIHFLRSEICLGPDGWISPSK
                     IIPNLADLL"
     gene            complement(343..1617)
                     /locus_tag="VFF70_05675"
     CDS             complement(343..1617)
                     /locus_tag="VFF70_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44226.1"
                     /translation="MTTKTSRHIPFAALRHRDYRLLWTGQLISQTGSTMQSLTINWHI
                     SVLTNYNPLALGLVGLARVIPIVFFSLIGGAVADARDRRKLMILTQSAMAIFAAVLAF
                     LTDHGLDIIWPIYVLNACSAAAWAFDNPARQALIPSLVPREHLTNAMSLNSIMFQTAT
                     IVGPTLAGFLIAGHSISIVYWINSLSFIAVIVALLFMHPVKVATRKVDLGSVREGIAF
                     VRHQPIIWSTMTLDFIATFFSSASQLLPIFAAKILNVGAQGFGILEAAPAIGALLSGG
                     TLSVFIMRIKRPGALILWSVALYGLATLAFGISTSFILSLIFLGLTGIADNVSTILRS
                     TIRQLVTPDHIRGRMTSVNMIFFMGGPQLGELEAGVVAALIGAPLSVITGGLGCLIAV
                     ALVAWKVPMLRKFDEDDLMRAGEALAVGAMRK"
     gene            1732..1923
                     /locus_tag="VFF70_05680"
     CDS             1732..1923
                     /locus_tag="VFF70_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZY44227.1"
                     /translation="MLKVTYRDQHWAVRGNQTVRELIQQVGLDPATVIAVRDGKLVFD
                     KELLGEDDEIKLIAVISGG"
BASE COUNT          473 a          544 c          524 g          389 t
ORIGIN      
        1 tctgtacgtt gacttcgccc gcgcccatct tcgatctctt atcagatcat tcgttagcgg
       61 tgctgctcgc tgaagagatc gaaatgctgc tggccgaacg gcgcgcggct tgggtcgcac
      121 agccgaactt gttcgatgaa cggctggccc gcaccgggcc gtggttattg tttgccgctt
      181 gtctcaaagc gttgtgggta cgctacgctc attacagcgt atcgattgac gatcccgatt
      241 atcacttaat tcacttcttg cgatctgaaa tctgtcttgg ccccgatggc tggatctcac
      301 cttcgaagat cattccaaat ttggccgatt tgctgtaacc gcttacttcc gcattgcccc
      361 aacggccaac gcttcccccg ctcgcatgag atcatcttcg tcaaacttgc gcagcatcgg
      421 cactttccag gccacgagcg cgacggcgat caaacaaccg agtccgccgg taatcacact
      481 caacggcgcg ccgatcagcg ccgccacgac acccgcttcc aattcgccca actgcgggcc
      541 acccatgaag aagatcatat tgacagacgt cattcgcccg cgaatatgat cgggggtcac
      601 gagctgccgg atggtcgatc gcaagatcgt gctgacatta tctgcgatgc cggtcaggcc
      661 cagaaagatc agcgacaaga tgaacgaagt cgagatgcca aaggcaagcg tcgccaagcc
      721 atacagcgcg accgaccaca aaatcaacgc gcccggccgc ttaatgcgca tgataaacac
      781 actcaatgtg ccgcccgata gcaatgcgcc aatcgccggg gccgcttcca ggatgccaaa
      841 gccctgcgcg ccgacattca aaatcttcgc cgcaaaaatt ggcagcagct gactggccga
      901 agagaagaag gtcgcgatga aatcgagcgt catcgtcgac cagatgatcg gttgatggcg
      961 cacaaacgcg atgccttcgc ggaccgaacc cagatcgact ttgcgcgtgg cgactttgac
     1021 cggatgcatg aataacagcg cgacgattac cgcgatgaaa gatagtgagt tgatccaata
     1081 tacgatcgag atgctgtgac cggcgatcaa aaagcccgcc agcgtcggac cgacgatcgt
     1141 cgcggtctgg aacatgatcg aattgaggct catggcattc gtcagatgtt cgcgcggcac
     1201 cagactggga atcaaggcct ggcgcgccgg attatcaaaa gcccaagcgg ccgccgaaca
     1261 ggcattcagg acgtaaatcg gccagataat gtccaagccg tgatcggtga gaaacgccaa
     1321 gacggcggcg aagatcgcca tggccgattg agtcaggatc atcaacttgc gtcgatcgcg
     1381 tgcatcggcc acggccccgc caatcagcga aaagaaaacg atcgggatga cgcgcgctag
     1441 accgaccaag cctaaagcca gcggattgta gttggtcaac accgagatgt gccaattgat
     1501 cgtcaggctt tgcatggtcg agcccgtctg cgaaatcaac tggccggtcc ataacaagcg
     1561 atagtcgcga tgtcgcaggg cggcaaatgg aatgtggcgg gatgtctttg tcgtcataga
     1621 gaatgaatgc aaatcctcag caagcaaact ataccacagc gcaccttgcc aaccgacgat
     1681 cagcggagat ttttgtcttg acgctgactt tctttctgtt agaatcatct catgctcaaa
     1741 gtaacctatc gcgatcaaca ctgggccgtg cggggcaacc agaccgtgcg tgaattgatt
     1801 cagcaagtgg ggcttgatcc ggcaacggtc atcgccgtgc gcgatggcaa attggtgttt
     1861 gataaagaac tgctcggcga ggatgatgag atcaaattga tcgccgtgat ttcgggcgga
     1921 taatctgatc
//
LOCUS       DASRTJ010000277         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_15995,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000277 DASRTJ010000000
VERSION     DASRTJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15995"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFF70_05685"
     CDS             <1..590
                     /locus_tag="VFF70_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZY44228.1"
                     /translation="FMQLLIQQAECTVEGMEGLVAYMHNQSDETADRVRKTEKAADEV
                     RRILIDELNRTFVTPLDREDIFALSRTIDDILDYAYSTVDEMEILDVKPNSYLQRMAS
                     LMRDAAEEIRLAVLRLKDHPGVANDHAVRAKALENRMEGVYREAIAALFQAPHDVEHI
                     VEMLKLREIYRHMSNAADRGDEAANVISDAVVKIV"
     gene            605..1432
                     /locus_tag="VFF70_05690"
     CDS             605..1432
                     /locus_tag="VFF70_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZY44229.1"
                     /translation="MKSDKSNVLKNHWVQPGHTVDLKDYDPNDTGPYRDKDDALDDLE
                     KDRLKLIELQGRLYAEKKRSLLLVLQGTDTSGKDGTIRHVMSGVNPQGVHVTGFEVPS
                     ADELAHDYLWRVHHATPTRGRIGIFNRSHYEDVLVVRVHNLVPRKVWQARYQQINDFE
                     RMLAENDTTVLKCFLYIDKDEQKQRLQERLDDPQKNWKFQKGDLADRKLWNEYMQAYE
                     DALSKCSTVIAPWIVVPANRKWYRNFVVMRAVVETLEQMDPQYPPPAPGLDKVVIED"
     gene            1576..1648
                     /locus_tag="VFF70_05695"
     tRNA            1576..1648
                     /locus_tag="VFF70_05695"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1609..1611,aa:Ala,seq:cgc)
     gene            1670..1742
                     /locus_tag="VFF70_05700"
     tRNA            1670..1742
                     /locus_tag="VFF70_05700"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Ala,seq:ggc)
BASE COUNT          444 a          432 c          517 g          377 t
ORIGIN      
        1 gctttatgca gctgctgatc cagcaagccg agtgcacagt ggaaggtatg gaaggtctgg
       61 tggcttacat gcacaaccaa tcggacgaga ccgccgatcg ggtacggaaa accgaaaaag
      121 ccgctgacga agtgcgccgc atcttgatcg atgaactcaa tcgcaccttc gtcaccccgc
      181 tcgatcgaga agacattttt gccttatcgc gcacgatcga cgacattctc gattatgcct
      241 actcgacggt cgatgagatg gagatcctgg acgtgaagcc gaactcgtat ttacagcgca
      301 tggcttcgtt gatgcgcgat gcggccgaag aaatccgatt ggcggtgctg cgcctcaaag
      361 atcatccggg cgtggccaac gatcatgcgg tgcgcgccaa ggcgctggag aatcgcatgg
      421 aaggcgtgta tcgtgaggcg atcgccgcgc tatttcaagc gccgcacgat gtggagcaca
      481 tagtggagat gttgaagctg cgcgagattt accggcatat gtccaacgcg gccgatcgcg
      541 gcgacgaagc ggcgaatgtg atcagcgatg cggtggttaa aatcgtataa accgggaggt
      601 cgaagtgaaa agcgataaat cgaatgtgct aaaaaaccat tgggttcagc cgggccacac
      661 agtcgatctg aaagattatg atccaaacga caccggccca tatcgtgata aggatgatgc
      721 gctcgacgat cttgaaaagg atcgattaaa gctgattgaa ttgcaagggc ggctgtatgc
      781 cgagaagaaa cgcagtctat tgttagtttt gcaaggcacc gatacttccg gcaaagacgg
      841 cacaatccgg cacgtgatga gcggcgtcaa tccgcagggc gtgcacgtga cgggctttga
      901 agttccatcg gcggatgaat tggcgcatga ttatttgtgg cgcgtgcatc atgccacacc
      961 tacccgcggc cgcatcggca tcttcaaccg atcgcattac gaggatgtgc tggtcgtgcg
     1021 cgtacacaat ctcgtgccac gcaaagtctg gcaggcgcgc tatcagcaga tcaatgactt
     1081 cgagcgcatg ctggcggaga acgatacgac tgttttgaaa tgtttcctct atatcgacaa
     1141 ggacgagcag aagcagcgat tgcaagaacg cttagacgat ccgcagaaga attggaaatt
     1201 ccagaaaggt gatctggccg atcgcaaact ctggaatgaa tatatgcagg cttatgaaga
     1261 cgctctcagc aaatgcagca ctgtgatcgc gccgtggatc gtcgtgccgg ccaatcgcaa
     1321 gtggtatcgg aattttgtcg tgatgcgcgc cgtcgtcgaa actttggagc agatggatcc
     1381 gcagtatccg ccgcccgcgc cgggtttgga taaagttgtg atcgaggatt gaaggactaa
     1441 ggagagaatg agggaaagag ggaatgagtg aaaaacatct ctccatctct ctttcgctct
     1501 atcgctcctt cgctctttat ttccatcaca tttgacacgg tgtaatgact caggtacaat
     1561 cctgaacacg atgttgggcc tgtagcgcag ctgggagcgc gcctcaatcg cactgaggag
     1621 gtcaagggtt cgaatccctt caggtccact gtgttaaatg aagaccattg ggcccatagc
     1681 gcagttggga gcgcgtctca atggcattga gaaggtcgag ggttcgaatc cctctgggtc
     1741 cacacaaacg taggggcgag gtgacctcgc
//
LOCUS       DASRTJ010000278         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_91381,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000278 DASRTJ010000000
VERSION     DASRTJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91381"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFF70_05705"
     CDS             <1..987
                     /locus_tag="VFF70_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44230.1"
                     /translation="RLPRVYDLAYRLSDRSSIDRVLRGPRQRAQQLSARSLLHAIQNV
                     KPDALICTHFLPAELCAGWRRSHQLSAPLVTVITDFEPHRMWQHLGTNGYCVATEEAA
                     DRLMLDGIDRSMIHVTGIPIRSEFAGLPDRVTARNRLQIDPDRSLLVVIGGGLGVGGI
                     DRVARLLIQQPIESQVAIITGRNRSLRRSLRSTSPDWIVRGFVENMPDWLAAADVVIS
                     KAGGLAAAELMAAGVPTIIPRGLRGHETNNAHYLLTHAAALMADSPQQAVERAIDLLQ
                     DHSLQNQMRSASLKFSKPNAAEDVADYVLRGSVPSLRAKATLAPHCVLVQCR"
     gene            1111..1746
                     /locus_tag="VFF70_05710"
     CDS             1111..1746
                     /locus_tag="VFF70_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44231.1"
                     /translation="MWKICLYQPADRSAVRQLCADTAFFGEPLEKFFDARELFLDYFA
                     TYYTDVAGDHLWVAAEENGSIAGYIMGCPDTDAYGRWLTQHLKQLKRKFFTLRYHGLT
                     PRTLRLIWNYGHLRGPAFVDLTPYPAHFHINTRSDLRGHGIGSALMETYLNQLRDEQI
                     SGVHLETTSENKIAVPWYEKLGFKLLSSWQTDLYRKSVGHKIDLLVYGMKL"
     gene            1799..2311
                     /locus_tag="VFF70_05715"
     CDS             1799..2311
                     /locus_tag="VFF70_05715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY44232.1"
                     /translation="MSFWKHLVAILALPVSVTIAIPIAIILSTHDLNVGWSLPEPITF
                     LPLVIGGVLIGTGLILMIKTISLFTTVGQGTLAPWAATKKLVVQGIYRYVRNPMISGV
                     FCISLGEALFFGSSTLLMWTIVVALINSIYMPLIEEPGLERRFGAEYRLYKKNVPRWI
                     PRRKPWPDME"
     gene            2402..3256
                     /locus_tag="VFF70_05720"
     CDS             2402..3256
                     /locus_tag="VFF70_05720"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZY44233.1"
                     /translation="MTANYSTDYTLDELMSVCISRQIVNGEVIGQGIATPLVMAGVLL
                     AKLSHAPDIYFASAIGGAVVRNWAPLSLTHVEKPWLDHALALVTFGQIACELLPTFKV
                     KEFFRPAQIDRNGNFNNVMIGDNYDRPQLRLPGSGGIADVTGYNDKTMLYVPRHSRAV
                     FVEKVDFISGLGYAPTRSTDLGPKVCITNLGQFDFADGLLRLTSFHAGQSIEKVQAKT
                     GFELQIAPDVCETEPPTTEEVRLLREEIDPLSVRKMETLSGAQRKQLIREILQREKSY
                     ASRVTDFE"
     gene            3294..3794
                     /locus_tag="VFF70_05725"
     CDS             3294..3794
                     /locus_tag="VFF70_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY44234.1"
                     /translation="MDIESIRELYNYHYWARDRVLAAVELITADQFTQSTGASFDTLQ
                     GTFVHLMNSERLWLNRWLGKADEPALEPRNFSMLNEVRMYWDMIQMHMEKYRKELTPD
                     RLSAVIRYTNLKGDTLQYPLWQMMQQVVLHGVHHRSEAATMLTEFGHAPEPLDLIFYF
                     REKSGQ"
     gene            3886..>4399
                     /locus_tag="VFF70_05730"
     CDS             3886..>4399
                     /locus_tag="VFF70_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44235.1"
                     /translation="MATDEKKVPPRPPWRERWQSVKKAAGNIPAAFQLAWDAHRRATI
                     GMAAWTIIGALIPAAQAWVGKLIIDSVVGSINGNIDATVGLTATLPYLAIEFVLILLQ
                     SGLAQARSLTEHILHARLNFTLNTRLIRKALSLDLSHFENAEFYDKLQNARREADYRA
                     LQIMNDGFYFI"
BASE COUNT          967 a         1262 c         1169 g         1001 t
ORIGIN      
        1 cgcttaccgc gagtgtatga tctcgcctat cggttatccg atcgatcttc gatcgatcgc
       61 gtgctgcgcg gaccgcgcca acgtgctcag caactcagcg cccgatcgtt gttacacgcg
      121 atccagaatg tcaaacccga tgcgctgatc tgcacgcact ttctgcccgc cgaactgtgc
      181 gcgggctggc gacgatcgca tcaactctcc gcgccgttgg ttaccgtcat caccgacttc
      241 gaaccgcatc gcatgtggca gcacctcggc accaatggct attgtgtcgc caccgaagaa
      301 gccgccgatc gattgatgct ggacgggatc gatcgatcca tgattcacgt caccggcatt
      361 cccattcgat ccgaattcgc cggcttgccc gatcgggtga ccgcgcgaaa ccgtttacag
      421 atcgatccgg atcgatcact gctggtggtg atcggcggtg gactcggcgt gggcgggatc
      481 gatcgcgtcg cccgattgtt gatccaacag ccgatcgaat cacaagttgc catcatcacc
      541 ggccgcaatc gatcgctgcg ccgcagtttg cgctcaactt cgccagactg gattgtgcgc
      601 ggctttgtcg agaacatgcc ggactggctg gccgcggccg atgtggtcat cagcaaagcg
      661 ggtggactcg ccgccgcgga attgatggcc gccggcgtgc caacgattat tccgcgcggc
      721 ttgcgcggcc acgaaacgaa caacgcgcac tatctgctca ctcacgccgc tgcactcatg
      781 gccgattcgc cgcagcaagc cgtcgagcgc gcgatcgatc tcttacaaga tcactcgctt
      841 caaaatcaaa tgcgttcagc ctctttgaaa ttttccaaac ccaacgcggc ggaggacgtt
      901 gcagattacg tgttgcgtgg ttcggttccg tcattgcgag caaaggcgac acttgcaccg
      961 cactgcgtgc ttgtgcagtg caggtgagca atctcccgat cgattatgag acacttgcac
     1021 cgcgctgcga aggcagtgca ggtgttgctt cgtcgcggag tttatcctga gcgaagtctt
     1081 aggactccgc aatgacgcct gataaagatc atgtggaaga tttgcctcta ccagcccgcc
     1141 gatcgatccg ccgtgcgcca gctgtgcgcc gacactgctt tcttcggcga gccgctggaa
     1201 aaattttttg acgctcgaga attattcctt gactacttcg ccacctatta cacggacgtc
     1261 gccggcgatc atttgtgggt cgccgcggaa gagaacggat cgatcgccgg ttacatcatg
     1321 ggctgcccgg atactgatgc gtatggtcgc tggttgacgc agcaccttaa acagctcaaa
     1381 cgtaaattct tcacactgcg ctatcacggc cttacgccgc gcacgctgcg actgatctgg
     1441 aattacgggc atctccgcgg cccggccttt gtcgatctca cgccctaccc ggcccacttc
     1501 cacatcaaca cgcgatcgga tctgcgcggc cacgggatcg gcagtgcgtt gatggaaact
     1561 tatctgaatc aattgaggga tgagcagatc agcggggtcc acctcgaaac aaccagcgag
     1621 aataaaatcg ccgtgccgtg gtatgaaaag ttaggcttca aacttttatc gagttggcag
     1681 accgatctgt accgtaaaag cgttggacac aagatcgatc tgcttgtgta tggaatgaag
     1741 ttgtgatggc aatcaaaacg cgctgaactg atttgctcgg cgcattccat ttaatatcat
     1801 gtccttctgg aaacatctcg tcgcaatcct cgccttaccc gtttcagtca cgatcgccat
     1861 tccgattgcg atcattctct caacccatga cctgaacgtc ggatggtcat tgcctgaacc
     1921 gatcaccttt ttaccgcttg tgatcggcgg tgtgctgatc ggcacagggc tgatcctcat
     1981 gatcaaaacg atctcgcttt tcaccacggt cggacaaggc acactcgcgc cgtgggcagc
     2041 gacaaagaaa ctcgtcgttc agggcatcta tcgctacgtt cgcaatccga tgatcagcgg
     2101 cgtgttctgc atctcgttgg gcgaggcgtt gttcttcgga tcgagtacgc tgttgatgtg
     2161 gactatcgtc gttgcgctga tcaactcgat ctacatgccg ctgatcgaag agccgggact
     2221 tgagcggcga ttcggcgccg agtatcggct ctacaaaaag aatgtccccc gctggattcc
     2281 tcgacgaaag ccgtggcctg acatggagtg aatttactta ccgcttgatt taacccgcgc
     2341 tttagtggat aatctttgtg ttgctttcca aaactactag tcctacaagt ccatctcatt
     2401 tgtgaccgct aactattcca ccgactacac cctcgacgaa ttgatgtccg tctgcatttc
     2461 gcgccagatc gtcaacggcg aagtgatcgg tcagggcatc gcgacgccgc tggtcatggc
     2521 cggcgtactg ctggctaaac tttctcacgc gcctgacatt tattttgcgt cagcaatcgg
     2581 cggcgcggtc gtgcgcaact gggcaccgct cagtttgacg catgtcgaaa aaccgtggct
     2641 cgatcacgcg ctggcgctgg tcaccttcgg gcagatcgcg tgcgagttgc tgccgacttt
     2701 caaagtcaaa gaattcttcc ggcccgcgca gatcgatcga aacggcaact tcaacaacgt
     2761 catgatcggc gacaattacg atcggccgca gctgcgcctg ccgggcagcg gcggcatcgc
     2821 cgatgtgacc ggctacaacg acaagacgat gttgtatgtg ccgcgtcatt cgcgtgcggt
     2881 atttgtcgag aaagtcgatt tcatcagcgg cctgggatat gcgccaactc gatcgaccga
     2941 tctcggtccg aaggtttgca tcaccaatct tggtcaattc gattttgcgg acggcctgct
     3001 gcgcttgacc tcttttcatg ccggacaatc gatcgagaag gtccaggcca agaccggctt
     3061 cgaattacag atcgcgccag atgtatgtga gactgaacca ccgactacgg aagaagtgcg
     3121 tctgctgcgc gaagagatcg atccattgag cgtgcgcaag atggaaacgt tgagcggcgc
     3181 tcaacgcaaa cagttgattc gagaaatatt gcagcgcgag aaaagttatg cgtcgcgcgt
     3241 gaccgatttc gaataaacct acctcactta cacttcatca gcaggagcat aggatggaca
     3301 ttgaatcgat tcgcgaattg tataactatc attactgggc gcgcgatcga gtgttggccg
     3361 ccgtagagtt gatcaccgcc gatcaattca cccaatcgac aggcgcgagt ttcgatacgc
     3421 tgcaaggcac ctttgtgcac ttgatgaact ccgaacgcct gtggctcaat aggtggttgg
     3481 gcaaagccga cgagccggcg ctcgaaccca gaaatttctc tatgctcaat gaagtccgca
     3541 tgtattggga tatgatccag atgcatatgg aaaagtatcg aaaagaacta acgcctgatc
     3601 ggctaagcgc tgtgatccgc tacaccaact tgaaaggcga cacactccaa tatccgctgt
     3661 ggcaaatgat gcaacaagtg gtgctgcacg gtgtgcatca tcgcagcgaa gcggccacca
     3721 tgctgaccga gttcggccat gcgcccgagc cgctggattt gatcttttac tttagagaga
     3781 agagcggaca atagaatatt tgtaagcgtc cttcgactgc gatgtgaatt cttcgacttc
     3841 acatctccac tctggatgct ctctctatct tattggagat agaacttggc aactgatgag
     3901 aaaaaagttc caccgcgccc accctggcgc gaaagatggc agagcgttaa aaaagccgcg
     3961 ggcaatattc ccgctgcatt tcagctggcc tgggacgcgc atcggcgcgc aaccatcggc
     4021 atggctgcct ggacgatcat cggcgcattg atcccggccg cgcaagcctg ggtcggtaaa
     4081 ctgatcatcg actcggtcgt cggatcgatc aacggcaata tcgatgcgac cgtcggacta
     4141 accgcgaccc tgccctatct cgcgatcgag ttcgtgttga ttctgctgca atccggtctc
     4201 gcccaggcgc gatcgctgac cgagcacatt ctgcatgccc gcctgaactt cacgctcaac
     4261 acgcgcttga tccgcaaagc cctgtcgctc gatctatcgc attttgaaaa tgccgagttc
     4321 tacgacaagc tgcaaaacgc ccgccgcgaa gcggattatc gcgccttgca gattatgaac
     4381 gacggcttct attttatcc
//
LOCUS       DASRTJ010000279         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_16254,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000279 DASRTJ010000000
VERSION     DASRTJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16254"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..960)
                     /locus_tag="VFF70_05735"
     CDS             complement(184..960)
                     /locus_tag="VFF70_05735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44236.1"
                     /translation="MTERNISFDRAAGFYDQTRKLAGSMATIGIQTILDLAGPHGSIL
                     EVGIGTGRIGVPLLQRGANLIGCDLSIEMMHQLHDKFPTARLVRSDAVRLPFPADRFD
                     ALITIHVLHLVGAWRIALRGFKRVLKSNGVYINSWHWHDSDSIDHKVRQHWRDQIEAR
                     GFNWRRPGIQSREELIAELKTMGARVEEKEIARGLDQTKPREILDGLTNRVFSDTWDL
                     PEDVLSASIRETHDWAIQELGDIDRPIDEEFRIILDVARF"
     gene            complement(961..>2159)
                     /locus_tag="VFF70_05740"
     CDS             complement(961..>2159)
                     /locus_tag="VFF70_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY44237.1"
                     /translation="FRTVYAHLTQALVSVGQQVKAGDKIGLADSTGNSFGSHLHITLK
                     LDGAQTPGYPAGIIDPWPYFQGVTLPTPATPTPPATPTGLKITTTDQLNLRAAPSSDG
                     AVLSTLQVNTSLSVLEDANGALGKIGRFGQWMKVQTSGNQQGYVAAWYVQADAKAAPA
                     SNIIAYTDDQLNLRSAPTTTGSILKILAEGEALTLLESIDTARSKIGLAGQWLNVRTT
                     TNQVGYVSAQYVHLTGQAPAATTLMIYATDIVNVRIQPAVTANSLTIISAGESLTVVG
                     DTTNAQAEIGQANKWVNVKTRAGFSGFVDAAKVSLTPKFTTPATNVQTLIVYPIDGVN
                     VRAQASTNSPIAGTALKNSALTVIESDPNAAKAKIGQQSQWVFVRTATGISGWVAAWL
                     LSLSQS"
BASE COUNT          487 a          590 c          573 g          509 t
ORIGIN      
        1 agtgcataac aatcgacggc ggcacgatga acagcgaagt gcgacgcact gctgaatgac
       61 agtgccgtga tctcgttaaa aagcgatcgg caaccttcat agccaccgat caccatcgcg
      121 ccgcaatatt cacatcgagc agtgctcatc gatcttgaat cgtttcttta atcgatctca
      181 atttcaaaac cgggcaacat ccaaaatgat tcgaaattcc tcatctatcg gtcgatcgat
      241 atcacccagt tcttgtatgg cccaatcgtg ggtctcgcga atggaggcgc tgagcacatc
      301 ctccggcaaa tcccacgtat cggagaaaac tcggtttgtc agaccgtcga ggatttcacg
      361 cggcttagtc tgatccagcc cacgagcgat ctctttctct tcgacccgcg cgcccatcgt
      421 cttcagctca gcgatcaatt cttcgcggct ttgaatgccg ggccgccgcc aattgaaacc
      481 acgtgcttcg atctgatcgc gccaatgctg gcgcactttg tgatcgattg aatctgaatc
      541 gtgccagtgc caactattga tataaactcc gtttgatttt aaaacgcgtt tgaaaccacg
      601 cagagcaatg cgccacgcgc ctacaagatg caaaacgtgg atcgtgatca gtgcatcaaa
      661 tcgatcggct gggaagggca atcggacggc atccgaacga accaagcgcg ccgtcggaaa
      721 tttatcgtgt agctggtgca tcatctcgat cgatagatcg caaccgatca gatttgcgcc
      781 acgctgtaac agcggcacgc cgatgcggcc cgtgccgatg cccacttcga ggatcgagcc
      841 gtgaggtccg gcaagatcga ggatcgtttg aataccgatc gtcgccatcg acccggccaa
      901 cttgcgggtt tgatcgtaga agccagcggc gcgatcgaat gaaatgttgc gttcagtcat
      961 tcaggattga gacagcgaca aaagccacgc tgcgacccag ccgctgatgc cggtcgccgt
     1021 gcggacgaat acccactggc tttgttgacc gattttggct ttcgccgcgt taggatcgct
     1081 ttcgatcaca gtcagcgcgc tgttcttcaa cgcagtgcct gcaatcggcg aattggtgct
     1141 ggcctgtgca cgtacattga ctccatcaat aggataaacg atcagtgtct gaacattcgt
     1201 ggcgggtgta gtgaacttcg gtgtgagcga aactttggcg gcatcgacaa agccgctaaa
     1261 gcccgcgcgc gttttcacat tgacccactt attcgcttgg ccgatctcgg cctgcgcgtt
     1321 ggttgtatcg ccgacgacgg taagcgattc accggcagat atgatcgtca aagagtttgc
     1381 ggtcacagcg ggttgaatgc gcacattgac gatgtcagtc gcataaatca tcagcgttgt
     1441 agcagccggt gcttgaccgg tgagatgcac atactgcgct gaaacatagc cgacttgatt
     1501 cgtcgtcgtt cggacgttga gccattggcc ggctagcccg atctttgatc gcgccgtgtc
     1561 gatcgattcc agtaaagtga gtgcttcgcc ttcagccaat attttcagaa tgctgccggt
     1621 ggtggtgggc gccgaccgca aattgagttg atcgtcggtg taggcaatga tgttcgaggc
     1681 aggcgcggcc tttgcatccg cttgcacata ccacgccgcc acataacctt gttgattgcc
     1741 tgaggtctgt actttcatcc attgaccaaa gcggccgatt tttcctaatg cgccattagc
     1801 atcttccagc acactcaatg aagtattcac ctgcaaagtg cttaacactg ctccgtcact
     1861 tgaaggtgcg gctcgcagat ttaattgatc ggtcgtggtg atcttcaagc cggtgggcgt
     1921 cgctggaggt gtgggagttg ccggtgtcgg caaagtcaca ccctgaaagt acggccaggg
     1981 atcgataatg ccagccggat aaccgggcgt ctgcgcccca tcgagtttca gcgtgatatg
     2041 caaatgcgat ccgaatgaat tgccggtcga atcggccaac ccaattttat cgcccgcctt
     2101 cacttgctgg cccacgctga ccaatgcttg cgtcagatgt gcatagacgg ttcgaaaag
//
LOCUS       DASRTJ010000280         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_243576,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000280 DASRTJ010000000
VERSION     DASRTJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243576"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFF70_05745"
     CDS             <1..851
                     /locus_tag="VFF70_05745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44238.1"
                     /translation="ITSDRFSFETIVVDNASTDDSVAMVRSKYPNSIVITNLTNLGFT
                     GGNNLGIQAAHGRYVMVLNPDAEIVDAAIDQMIDYLDAHAEVGAIGPQLLNPDRSVQS
                     SRRRFPTMLTGIFESTWLQGIAPRNVLHRFYMDDVSPDAVQEVDWLTGACTIFRRSVL
                     DQVGIYDEANFFMYSEEIDLCRRIKAASHSIVYLPAAQVIHFIGQSSDQVVAARQLYF
                     QTSKVNYFRKHHGRFKAGLLRLFLLTSYLWQIVLESTKGLLGNKRSMRRERVKVYWQV
                     VRSGLR"
     gene            848..>1676
                     /locus_tag="VFF70_05750"
     CDS             848..>1676
                     /locus_tag="VFF70_05750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY44239.1"
                     /translation="MKIGLITGEYPPMQGGVGNFTRELTRAMINLGHDAQVITQKGVG
                     GDEHNGIVVHRVVDSWGLKCWRQIAKVARDNHLEILNIQYEPAAYSMRVGINFLPSAF
                     ARRTIKLPIVTTFHDLLVPYLFPKAGSLRRKVVEYLAHHSDAAIVTNAEDRSKLESFG
                     VAQDRPPISNLHVIPIGSNIDPSRLIDFDSQATRARWSLQPNEMLIGYFGFLSLSKGG
                     EVLMRALKLLVDRNLPVKLMLIGGRTGSSDPTNAEYAARVDRLINSLGLKDRVLTTVY
                     "
BASE COUNT          396 a          396 c          461 g          423 t
ORIGIN      
        1 cgattacatc cgatcgattt tcgttcgaaa cgattgtggt tgacaatgct tcgactgacg
       61 atagcgtcgc gatggttcga tcgaaatatc cgaattcgat cgtgattacc aacttgacga
      121 atctcggttt cacaggtggc aacaatctag ggattcaagc ggcgcacggt cgatatgtaa
      181 tggtgctcaa tcctgatgct gaaatcgttg atgctgcaat cgatcaaatg atcgattatc
      241 ttgacgcaca cgcggaggtg ggcgcgatcg ggccgcagtt actgaatccc gatcgatcgg
      301 tccagtcttc acggcggcgt ttcccaacga tgctgacggg tatctttgaa agcacatggc
      361 tgcaaggcat cgcgccgcga aatgtgttgc atcgttttta catggatgac gtttcacccg
      421 acgcagtgca ggaagttgat tggctgaccg gtgcctgcac gatctttaga cgtagtgtgc
      481 tggatcaagt tggcatctat gatgaagcaa acttcttcat gtattcggaa gaaatcgatc
      541 tttgccgacg catcaaagcc gcgagccatt cgattgttta cctgcctgcc gcgcaagtga
      601 ttcacttcat cggccagagc agcgatcaag ttgtggccgc gcggcagctg tattttcaaa
      661 cgagcaaggt gaattatttc cgcaaacatc acggtcgatt caaggccggg ctgctgcgtc
      721 tgttcttgct gacttcatat ctgtggcaga tcgttttgga gtcgacgaag gggctgctgg
      781 gcaacaaacg ctcgatgcgg cgtgaacgcg tgaaggtata ttggcaagtt gttcgatcgg
      841 ggctgcgatg aagatcggat tgattacggg tgagtatcca ccgatgcagg gcggcgtggg
      901 taatttcacg cgtgaattga cccgcgccat gatcaatctg ggtcatgacg cgcaggtgat
      961 aactcagaaa ggggtagggg gcgatgagca taatggaatc gtcgtgcatc gagtagttga
     1021 ttcatgggga ttgaagtgtt ggcggcaaat tgcgaaagtc gcacgcgata atcatttgga
     1081 aatactgaat attcaatatg agccggccgc ttactcgatg cgcgtgggca tcaacttttt
     1141 gccgagtgca tttgcacgac gcacaatcaa acttccaatc gtcacaacgt ttcatgattt
     1201 gctggtgcct tatctttttc ccaaagccgg atcgctgcgc aggaaagttg tcgagtatct
     1261 tgcgcatcac agcgatgcgg caattgtcac caacgcagaa gatcgatcca aactcgaatc
     1321 cttcggcgtc gctcaggaca gacctccaat ttctaacctc cacgttatac ctattggcag
     1381 caacatcgat ccaagccgct tgatcgattt tgattcacaa gcaactcgcg ctcgttggag
     1441 cctacaaccg aatgaaatgc tgatcggcta cttcgggttt ttgagtttaa gcaaaggcgg
     1501 cgaagtattg atgcgcgcgc tgaaattgct cgttgaccgg aacttaccgg tcaagctgat
     1561 gttaatcggc gggcgaacgg gctcaagcga tccgacgaat gctgaatatg cggcgcgggt
     1621 tgatcggctg atcaattcgc ttgggttgaa agaccgcgtt ctcaccacgg tctatc
//
LOCUS       DASRTJ010000281         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_243585,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000281 DASRTJ010000000
VERSION     DASRTJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243585"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFF70_05755"
     CDS             complement(<1..833)
                     /locus_tag="VFF70_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44240.1"
                     /translation="MLKLRRPIIFLIIAFINVALYVGVSASRGHVGYPLDDAWIHQTY
                     ARNWAETGQLAYVIGQPSAGSTSPLWTLLISLAYRLNIDPVAWTLLLGALCLALSASL
                     LARIADRLLPNRKLIAWLVGLACLFEWHMIWATASGMETMLFIALALIVIDRLLADAR
                     AWSIGFFGGLLILTRPEGLLLIGLAALLLLTRSANGGPKEFLLTIIIALVVVTPGALI
                     NFQASGTFFPNTFYAKQQEYAELMSSASIWLTSIGNMIGAPFVGSQVLLLPGVFYWLI
                     YG"
     gene            complement(837..2465)
                     /locus_tag="VFF70_05760"
     CDS             complement(837..2465)
                     /locus_tag="VFF70_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44241.1"
                     /translation="MNTDPVSNRLRRDRPALRHAVGQVWIVLFLALIIRLVAALPQSQ
                     PNYMDATYYTVGGQRLAQGYGFNDPYVWNYLDQPTTLPHPSHVYWMPLASMLVGISES
                     IFGISYRAAQIPFIVLSALLSLIAFSVAWRLSSSRRAAWIAALLTIFSGFYVPYWGVP
                     ESFAPFAVFGSLALYLSVDRTARRLFLAGLCAGLAHLSRADGVLLLIPMLWIQFRDEG
                     GAMKDEKRQSYRFRPDLSPLIFIFMGYFLIMLPWFIRNSVAIGAPLSAAGTQAAYVCN
                     YDELFSFNAQLDLNHLINCGAGQVLLDKLTGLGTNLIHLMAEDGLIFLAPLIAIGVWK
                     LRRERVYQAVIIYLVLLYLVMSIIFTFAGERGGLFHSSSALLPFFFAAVPIGLDAIID
                     RIAARRRTWNARQARSIFGSFIVILAVALSVSLWFGRVIGANVADPIWNQADRVYDSI
                     GQWLTDQGEHDPIVMVNNPPSFYYHTGLRSIVIPAGDVNPLLLAADQFGAGWLVLDPN
                     HVHALDALYAGSEINPRLEHVNDFASIQVYRIVH"
     gene            complement(2484..>2767)
                     /locus_tag="VFF70_05765"
     CDS             complement(2484..>2767)
                     /locus_tag="VFF70_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44242.1"
                     /translation="YRSNVLDSINFRANSDKFIFDQEIVAQIVECGYRIAEIPVPVRY
                     FAEASSASFFDSTLYGLSIVWLLIRYALHRIGIRKSQQFTCLGQRYQKV"
BASE COUNT          757 a          734 c          728 g          548 t
ORIGIN      
        1 ccataaatga gccaataaaa tacaccgggc aacaatagca cttgactacc tacaaaggga
       61 gcgccgatca tatttccaat gctggtcagc cagattgatg cgctgctcat gagttcagca
      121 tactcttgtt gcttggcata gaacgtattc ggaaaaaaag ttccgctggc ttgaaagttg
      181 atcaacgcgc ctggcgtaac caccacgagc gctatgatta ttgtgagcaa aaattctttc
      241 gggccgccat tagctgatcg ggttagcagc agcagcgcag ctaaaccgat caacagtaaa
      301 ccttctggcc gcgtcaagat caataatccg ccgaagaatc cgatcgacca agcccgcgca
      361 tctgccaata atcgatcgat gacaatgagc gccaatgcta tgaatagcat cgtttccatg
      421 ccggacgccg ttgcccagat catatgccat tcaaatagac acgctaaacc gacgagccac
      481 gcaattaatt ttcgattggg cagtaatcga tcggcgatgc gggctaacag cgaggcgctt
      541 agtgcaagac acagcgcgcc cagcaagagt gtccacgcca ccggatcgat gttcaaacga
      601 taggcgaggc tgatcagcag cgtccacaat ggggatgtcg aaccggcgct gggttggccg
      661 atgacgtagg caagctgtcc ggtctcggcc cagttacgcg cgtacgtttg atgaatccac
      721 gcatcatcca acggatagcc gacgtgacca cgtgacgcgg acacgccaac gtagagggcg
      781 acgttgatga aggcgatgat caagaagata atgggtcgtc gaagttttag catcagctag
      841 tgcacaatcc gataaacttg tatcgatgca aaatcgttga catgttcaag ccgcggattt
      901 atttcggagc cggcatacaa cgcatccagc gcgtggacat gattcggatc gagcaccagc
      961 caacccgcgc caaattgatc cgccgcgagc agcagcgggt tcacatcgcc ggccggaatg
     1021 acgatcgatc gcaagccagt gtgataatag aatgatggcg gattgttgac catcacgatg
     1081 ggatcatgct caccttgatc ggtcaaccac tgcccgattg aatcataaac ccgatcggct
     1141 tgattccaaa tgggatcagc gacatttgcc ccaattactc gaccaaacca gagcgacacg
     1201 cttaacgcta ccgccaagat tacaatgaat gatccgaaga tcgatcgggc ttgccgcgca
     1261 ttccatgttc ggcgacgagc ggcgattcga tcgatgatcg catccagtcc aatcggcaca
     1321 gcggcgaaga agaatggcag caacgcgctg ctcgaatgaa acaagccacc gcgctcgccc
     1381 gcaaaagtaa agattatcga catcaccaag tagagtaata ccagataaat aattacggct
     1441 tgatacactc tctcgcgccg gagtttccac actccgatcg caatcagcgg cgcgaggaag
     1501 atcaagccat cttcagccat caaatgaatc agattggtgc ctaagcccgt cagcttgtct
     1561 aacagcactt ggccagcgcc acaattgatc aggtgattga gatcgagctg cgcgttaaaa
     1621 gaaaacagtt cgtcgtagtt gcagacgtaa gccgcttgtg tgcccgccgc tgacaacggc
     1681 gcgccgatcg ccacgctgtt gcgaatgaac cacggcagca tgatgaggaa atagcccatg
     1741 aagataaaga tgagaggtga gagatcaggc ctgaatctgt aactctgtct tttctcatcc
     1801 ttcatcgctc cgccctcatc cctgaattga atccataaca tgggaattaa caacagcaca
     1861 ccgtccgcgc ggctcaagtg cgccaagccc gcgcacaggc cagcgagaaa caatcgacgc
     1921 gcagttcgat cgacggataa atacaacgcc aacgatccaa acacagcgaa cggcgcgaag
     1981 ctttccggca cgccccaata cggcacatag aaaccgctga agatcgttag cagtgcggcg
     2041 atccaggcgg cgcgccgcga gctgcttaat cgccaagcga ctgaaaaggc aatcagtgac
     2101 agcaacgcgg agagaacgat gaatggaatt tgcgcggcgc gataactgat cccgaagatc
     2161 gattcgctga tcccgacaag catcgacgct agcggcatcc aatagacgtg gctgggatgc
     2221 ggcaacgtgg tcggttgatc cagataattc cagacatacg gatcgttgaa gccatagcct
     2281 tgcgctaagc gctgtccgcc aacggtgtaa taagttgcat ccatgtaatt gggttgagat
     2341 tgaggcagcg cagcaacgag gcgaataatg agcgcgagga ataaaacgat ccacacttgc
     2401 cccactgcgt ggcgcagtgc gggtcgatcg cggcgcagcc tgttgctcac gggatcagtg
     2461 ttcattgata attgttcatt gattcagact ttttggtagc gctgacccag gcaagtgaac
     2521 tgctggcttt tacgaatgcc gatgcgatgg agggcataac ggatcagcag ccagacaata
     2581 ctcaggccat acaatgtgct gtcgaagaag ctggccgacg aggcctcggc aaaatatcgg
     2641 acggggacgg gtatttcggc aatgcgataa ccgcactcga cgatttgggc gacgatttcc
     2701 tgatcgaaaa taaatttgtc ggaattcgcg cgaaagttga tactgtctaa cacattgctg
     2761 cgatagg
//
LOCUS       DASRTJ010000282         7663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_91908,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000282 DASRTJ010000000
VERSION     DASRTJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91908"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFF70_05770"
     CDS             complement(<1..190)
                     /locus_tag="VFF70_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44243.1"
                     /translation="MSFRIARSAVRNLYLDGHTRLIDIEIPRYARNDMMDMIDLIQKA
                     AELIARARHAVALTGAGIS"
     gene            complement(197..1336)
                     /locus_tag="VFF70_05775"
     CDS             complement(197..1336)
                     /locus_tag="VFF70_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44244.1"
                     /translation="MPAKILLVDDDLQTLKLIGLMLQRRGHIIVPATNGIEGLAKAMT
                     DTPDLVLLDIMMDDIDGFEVCRRLRANPDTARIPIIMFTAKSMVNDKVTGFQAGADDY
                     LTKPIHPNDLALRVETVLQRSLRFQSESDSTDRARIVGFLGVKGGVGTTTLAMNVGAA
                     LASTPPPRKVVLIDLQASAANVSVQLGLSPSSGLLALLHHAPDEIKPELVSQQLIAHP
                     SGLQLLLPSLDMRSNSETIPAAQMRAVLNSLSHISQAVLIDVGCIIDDNSAITLGACN
                     HIVLVLEPQRVAITVAQTMIAQLDRRGIPADRLIIAVVNRSPSALTIDKRTIESLLKL
                     PISVLIPPAPEAAYQAAEQAALMVYLQPHSIIVDQFRSLAQRIMQ"
     gene            1506..4382
                     /locus_tag="VFF70_05780"
     CDS             1506..4382
                     /locus_tag="VFF70_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZY44245.1"
                     /translation="MRYNASAMSPLGIALILLVVVIAIVLVMIVRRRLAAQRDLLGQV
                     QELSSLADFARAIAEARLNVDELCELIYQRATDIVDTSSFQLGLFEGEWYDIRVWLRD
                     GLRLPAERFDLHDNPGLIGWIRDSKQSLIVRDFEQEIDRLPARPRYTTELPPRSAVFV
                     PLISGDRAVGALSIQSHQPDAFTENHLRLLTIIGNQAAAAVANRRLFELERRRTAQMQ
                     LITEVNQQIASILDLETLFHQTAELIRATFSYSFVAICVREEDSNRIVFEGATDPALQ
                     DKRMHVGQGVIGWVVEHGEVLNVPDVSQDDRYWPQATLLETRSELAVPLIFGSDTIGA
                     LDVESNELNAFSDDDVYILRTLAGQIAIAIHEARLYAAEREQAWISTALLQVAEATGH
                     ATSLEEVLDTVARITPMLSGVERCGIMLLDGKQGIFHAQAAFGLETAADEFYDLQLTP
                     DSSPMLNALYQTNKPIMRPAATGNDPLSKYFGPGDVLGLPIMAHGDLIGVMWIGASPG
                     QMLSGRKAALIGGIANQAAMAIESAQLAVAQREEAWVSTALLQVAEATGSLTDLNEIL
                     STITRLTPLLVGVEVCAIFLWDAGRQAFVGNQAYGLPHEVQDKFESIKIAWAEWPLSE
                     ADRSVRGAAVPFVPDRLMQALRLDAPIALPLRARAEIVGALLVDRGTHELMLNQRRLN
                     ILTGIASQTAVAIENVHLMADLAARQLLEKELDVAREIQKSFLPGRAPNVPGYQIAAY
                     WRSARRVGGDFYDFMPLANQQWGLAIADVADKGVPAALFMALTRTLLRATAMSGRSPA
                     AALSRANELILSDARSDLFVTIFYGVLDPRSSKFTYSNAGHNPPIWFDARSSTTHFLK
                     AHGIALGVVPEVLVKDNKLTLTPGDVLLLYTDGVTEAFNVEGEEFGEKRLAQVVRDCA
                     ASTAEELSHAIVSAVRSFVGEEPAFDDVTMVVVKKDEGRMTNDE"
     gene            complement(4569..6026)
                     /locus_tag="VFF70_05785"
     CDS             complement(4569..6026)
                     /locus_tag="VFF70_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZY44246.1"
                     /translation="MTLMLRSSLIDFQTRRRSRTLRTLGILAVIVYVIWQFISFQLSL
                     TQLPATWRIADESFKDLPIDVALQQVSIDLQQPIALQYFTNTITLQPGSIDFALDADQ
                     TALTIHALRSQNALIDFMRRLIFQPPAPRDVAITASYSTEKVRAALAEFGSQLDQPAQ
                     PAKFQIDPANAIAHGQDGYQLNIIQSMKPIDAALKSATQREVTLVIEHQLAPPPSIDQ
                     LQQYIKDRIAPFPANASVFIKALQTGQEIDLNPNVAYSGLGVMKIAIMVETFRKLDDA
                     PDVTTTLRLTTSIVAENNFSANSLVQLIGDNDLNRGVDIVSASLHNLGLANTFMLAAY
                     GYTSTLPTLITPANSITTTHADPDPYMQTTASDTGLLLEAIYQCHRNGGMLMVVYPDK
                     FTSNECDQILNILKQTDPNAPSALRGGLLAGTSIAHKIGANADTRADASIVFSPGGDF
                     VLVVFFNTPNQPLEWSAVNPIMADVARTAYQFFNP"
     gene            complement(6149..7474)
                     /locus_tag="VFF70_05790"
     CDS             complement(6149..7474)
                     /locus_tag="VFF70_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44247.1"
                     /translation="MQPSSSTRSPLIVFIVGLILIIGGLIAVIVWGVAAFNQPHPVAT
                     QPIETVQSIGSATNRIAVIDQDQIYTIDPDGGNQASITVDGRVPTGALIWSRAGTRLI
                     YAATTATGSQLISVAPDDSDETILYAAERARVPFYLYGSPDDQHVAFLQSDPAANSLN
                     LQLTAISPTPTVQSIAIGQPDYYSWSPQSDALLLHVGGTSRAAFIGTYHLGANALQNI
                     NTTPALFQTPMWSPIDQRLLYARQYLEGGQLIVGDGARETAVITFEVGLAFSWSPDAN
                     HIAYTHSNLVNFTYTSLSIIDTGSQAAHQYFGGDILAFFWSPDGSKLAYLTGALIEPG
                     PVGKTGGLAAPRIDQQALRLTWHVIDLQTQQTINLMSFEPSDSFVFLVQYFDQFAQSI
                     EVWSPDSRSLVFTGAPFNGQSGVYVIDATKANSTPANVGSGDFAIWSWK"
BASE COUNT         1657 a         1984 c         2334 g         1688 t
ORIGIN      
        1 tgctgatgcc cgcgcccgtc aaggccacgg catgtcgtgc tcgggcgatt aattccgcgg
       61 ctttttggat gagatcgatc atatccatca tgtcattccg agcgtagcga ggaatctcta
      121 tatcgatcag ccgagtatgc ccgtcaaggt agagatttct caccgcgctg cgcgctattc
      181 gaaatgacaa aaagcgtcac tgcatgatcc gctgcgccaa acttctaaac tgatccacga
      241 tgatgctgtg gggctgcaaa taaaccatca gcgctgcttg ttcggctgct tgatacgctg
      301 cctcaggggc cggcgggatg agcacactga tcgggagttt caacaaactc tcgattgtgc
      361 gtttatcgat cgtcagcgcg ctgggcgatc ggttgacgac ggcgatgatc aatcgatcgg
      421 ccggaatgcc gcgccgatcg agctgcgcaa tcatcgtttg cgccacggtg atggccacgc
      481 gctgcggttc aagcaccaag acaatgtgat tgcacgcacc cagcgtgatg gctgaattgt
      541 cgtcaatgat gcagccgaca tcgatcagga cggcttgact gatatggctc aagctattca
      601 gcacggcccg catttgagcc gcagggatag tctcggagtt gctgcgcata tccagactgg
      661 gcaagagcag ctgcaggccg gacggatgcg cgatcaattg ttgactgacc agttcgggtt
      721 tgatttcatc cggtgcatgg tgcaacagcg ccagcaaacc cgatgagggt gacaggccta
      781 actgtacaga tacatttgcc gcactggctt gcagatcgat cagcaccact ttgcgcggcg
      841 gcggcgtgct ggccaaagct gcgccgacat tcatcgccag cgtcgtcgtg cccacgccac
      901 ctttcacgcc gaggaagccg acgatacgcg ctctgtcggt ggagtcgctt tcgctttgaa
      961 aacgcaagct gcgttgtaaa accgtttcga cgcgtaacgc caagtcgttg ggatggatcg
     1021 gcttagtgag gtaatcatcg gcgccagcct gaaaaccggt taccttatcg ttgaccatcg
     1081 acttggcggt aaacatgatg atggggatgc gtgcggtgtc aggattggcg cgcaagcggc
     1141 ggcagacttc aaagccgtcg atgtcgtcca tcatgatatc cagcaggacg agatcgggtg
     1201 tgtcggtcat ggctttggcc aatccttcga tcccgtttgt cgccggaaca atgatgtgcc
     1261 cgcgccgctg cagcatcagg ccgatcagct tgagggtttg gagatcgtcg tcgacgagca
     1321 gaatcttggc gggcatgcac gaactctagc acaaaagaga atcaatgaca aatgaaagat
     1381 gatcaatgac caacaacaaa tgaccaagtt gtgcgtcatg gctagtcgcg tgaagagcga
     1441 ctagccatcc cgctttgatg atcattgaga ttgcttcgcc tgtgcgggct cgcaatggca
     1501 gtttcatgcg gtataatgca agcgctatgt cgccgttggg tattgctctt attttgctgg
     1561 tggtggtcat cgccatcgtc ttagtgatga tcgtccgccg ccgcttagcc gctcaacgcg
     1621 atctgctcgg ccaagtccaa gaactttcct cacttgccga tttcgcccgc gccattgccg
     1681 aagcccggct gaatgtcgat gaattgtgcg agttgatcta tcagcgcgcc acagacatcg
     1741 tcgatacgtc ctcctttcaa ttaggcttat ttgaaggcga gtggtacgac attcgcgtgt
     1801 ggctgcgcga tggcctgcgc ctgcctgccg aacgcttcga tctgcacgac aaccccggct
     1861 tgatcggttg gatcagggac tccaagcaat cgttgatagt gcgcgatttc gaacaggaga
     1921 tcgatcggtt gccagctagg ccgcgctata ccactgagct gccgcctcga tcggccgtgt
     1981 tcgtcccatt aatcagcggc gatcgcgccg tcggtgcatt atcgatccaa agtcatcagc
     2041 ctgatgcgtt caccgaaaat catctgcgct tgttgacgat catcggcaat caagccgcgg
     2101 ctgccgtcgc caatcgccgc ctgtttgagt tggagcgccg ccgcaccgcg cagatgcagt
     2161 tgatcaccga agtcaatcag cagattgcct ccatccttga tctggaaacg ttgttccatc
     2221 aaacggccga actgattcgc gccacattca gttattcctt tgtggcgatc tgtgtgcgcg
     2281 aagaagattc caatcgcatc gtgttcgaag gcgcgacgga tccggcactg caggataaac
     2341 gtatgcacgt ggggcagggc gttatcggct gggtggtgga acatggcgaa gtgctgaacg
     2401 tgcccgatgt gtcacaagac gatcgttatt ggccgcaagc gactttgctg gaaacccgat
     2461 cggaattggc cgtcccgttg atcttcggca gcgacacgat cggcgcgctg gatgtggaaa
     2521 gcaacgaact caatgcgttt agcgacgatg acgtttatat tttgcggacg ctcgccggtc
     2581 aaattgcgat tgcgatccac gaagcgcggt tatatgccgc cgagcgcgaa caagcctgga
     2641 tcagcaccgc gctcttgcaa gtggccgaag ccaccggtca cgccactagt ttggaagaag
     2701 tcctggacac ggtggcgcgc atcacgccga tgttgtccgg tgtagaacgc tgcggcatca
     2761 tgctgctcga tggcaagcaa ggaatttttc acgcgcaggc cgcgtttggc cttgaaacgg
     2821 ctgccgacga gttttatgat ttgcaactga cgcccgatag cagtcccatg ctgaacgccc
     2881 tgtatcaaac caataaaccg atcatgcggc ctgctgccac cggcaacgat ccgctgagca
     2941 aatatttcgg gccgggtgat gtgctaggtt tgccgatcat ggcgcacggc gatctgatcg
     3001 gcgtgatgtg gatcggcgcg tcgcccggtc aaatgttgtc gggccgcaaa gctgccttga
     3061 tcggcggcat cgccaatcaa gccgcgatgg cgatcgaaag cgcgcagttg gcggtggcgc
     3121 agcgtgaaga agcgtgggtc agcacggcgt tattgcaagt agctgaagcg accggctcgt
     3181 taaccgatct caatgaaata ctcagcacga tcacgcgctt gacgccgctg cttgtcggcg
     3241 ttgaagtgtg cgccatcttt ttatgggacg ccggtcgaca agccttcgtc ggcaatcaag
     3301 cctacggtct gccgcatgaa gtgcaggata aatttgaatc gatcaagatc gcctgggctg
     3361 aatggccgct gagtgaagcc gatcgatcgg ttcgcggcgc ggcggttccg tttgtgcccg
     3421 atcggttgat gcaagctctg cggcttgatg cgccgatcgc cctgccgctg cgggcgcgcg
     3481 ccgagattgt cggggcattg ctggtcgatc ggggcacgca cgagctgatg ctgaatcagc
     3541 gccgcttgaa tatcttgacc ggcatcgcca gtcaaacggc ggtcgcgatc gagaatgtgc
     3601 atcttatggc cgatctcgcg gcgcggcagt tgcttgaaaa ggaactggat gtggcgcgcg
     3661 agattcaaaa gagtttcctg ccgggccgcg cgccaaatgt gcccggttat caaatagcgg
     3721 cgtattggcg atcggcgcgg cgggtgggcg gcgacttcta tgatttcatg ccgctggcca
     3781 accagcaatg gggtttggcg attgcggatg tggccgataa aggtgtgcca gctgcattgt
     3841 tcatggcttt gacgcgcacg ctgctacgcg cgacggcaat gagcggccgc tcgccggccg
     3901 cggccttgag ccgcgcgaac gaattgatcc tgtccgacgc gcgatcggat ttgtttgtga
     3961 cgatcttcta cggcgtgctc gacccccgat cgtcaaagtt cacgtattcg aatgcgggtc
     4021 acaatccgcc gatctggttc gatgcccgat cgagcacaac gcatttcttg aaagcgcatg
     4081 gcattgcgtt gggcgttgtg ccggaagtgt tggttaaaga taataagttg actttaacgc
     4141 ccggtgatgt gctgctgttg tataccgatg gtgtgaccga agccttcaat gtggagggtg
     4201 aagaatttgg cgagaagcgt ttggcgcaag ttgtgcgcga ttgtgcagcg agcaccgccg
     4261 aagaacttag ccacgcgatt gtgagcgccg tgcgatcttt cgtcggcgag gaaccagcct
     4321 ttgatgatgt gacgatggtg gtggtgaaga aggatgaagg aagaatgaca aatgacgaat
     4381 gaaaaatgac aaatgattaa atgaagacga tcgatgtgat gagcatcgat cgttttgttt
     4441 cgcgtcattg cgagcgaagc gaagcaatct cagtattgtt caggggattg cttcgttggc
     4501 ctaacggcct tctcgcaatg acgtggtttg atgtaatcac tcgcgatatt tgcgtggttc
     4561 ttgcacaact acggattgaa gaattgatac gccgttctag caacatccgc catgatcgga
     4621 ttgacggcgc tccactccaa cggctgattc ggcgtgttga agaagacaac taaaacaaag
     4681 tcaccgccgg gcgaaaacac gatcgacgcg tcggcgcgag tgtccgcgtt ggcgccgatc
     4741 ttgtgcgcga tcgatgtgcc cgccagcagg ccgccgcgca acgccgatgg cgcgttcgga
     4801 tcggtttgct tcaggatatt gagtatctga tcgcattcgt ttgaagtgaa cttatcggga
     4861 tacacgacca tgagcatgcc accgttgcga tggcactgat agatcgcttc aagcaacaaa
     4921 ccggtatcgc tggcagtggt ctgcatatac ggatcaggat cagcgtgggt cgtggtgatc
     4981 gaattggcag gtgtgatcag cgtaggcagc gtcgaggtgt aaccgtacgc cgcgagcatg
     5041 aaagtattcg ccaagcccaa attgtgcagc gaggcgctga ctatatccac gccgcgattg
     5101 aggtcgttat cgccgatcaa ctgcacgagc gagttcgcgc tgaaattgtt ctcggccacg
     5161 atcgaggtag tcagccgcag ggttgttgtg acatcgggcg cgtcatcgag tttgcggaag
     5221 gtttcaacca taatcgcaat cttcatcacg cccaggccgg agtaagcgac gttgggattg
     5281 agatcgatct cctggcctgt ttgcagagcc ttgatgaaga cactggcgtt ggcgggaaac
     5341 ggcgcgatgc gatctttgat gtattgctgc aactgatcga tcgaaggcgg cggcgcgagt
     5401 tgatgttcga tcaccagtgt gacttcgcgc tgcgtggccg acttcaacgc cgcatcgatc
     5461 ggtttcatcg attggatgat gttgagttgg tagccgtcct gtccgtgcgc gattgcattg
     5521 gccggatcga tctgaaactt ggcaggctgc gcgggttgat cgagctgtga accgaattct
     5581 gccaaagccg cgcgcacttt ctcggtcgag taactggctg tgatcgcgac gtcgcgcggg
     5641 gcagggggct ggaagatcaa tcggcgcatg aaatcaatta aagcgttttg cgatcgcagc
     5701 gcgtggatcg tcagcgcggt ttgatcggcg tccaacgcaa aatcgatcga gccgggctgc
     5761 agggtgatgg tattcgtgaa gtactgcagc gcgatcggtt gttggagatc gatcgagact
     5821 tgttgcagcg cgacgtcgat gggcagatct ttgaatgatt catcggcgat gcgccaagtg
     5881 gcgggcagtt gcgtcaatga gagttgaaac gaaatgaatt gccagatcac atagacgatg
     5941 actgcgagga tgccgagtgt gcgcagggtg cgcgatcgac ggcgggtttg gaagtcaatc
     6001 aatgaagacc tcaacattag ggtcattgcg aggagtgccg ttggcacgac gaagcaatct
     6061 caccttaggt ggatcggaag attgcttcgc ggcacatccc tagcgggctg tgccatcgtg
     6121 ggactttgct cgaaatgact cagcagggtt acttccacga ccagatcgca aaatcgccgg
     6181 agccgacgtt ggccggtgta ctattggcct tcgtcgcatc gatcacgtac acgccgctct
     6241 ggccgttgaa gggcgcgccg gtaaacacca acgatcgact gtcgggcgac cacacctcga
     6301 tcgattgcgc gaactgatcg aaatactgga ccagaaagac aaagctatcg ctgggctcga
     6361 agctcattaa attgatcgtc tgctgcgttt gaagatcgat cacatgccaa gtgaggcgca
     6421 aggcttgttg atcgatgcgc ggcgcggcga gcccgcctgt cttgccgacc ggacccggct
     6481 caatcagtgc gccggtgaga taggccaact tcgatccgtc cggcgaccag aagaatgcaa
     6541 ggatatcgcc gccaaaatat tggtgagctg cttggctgcc ggtgtcgatg atcgagagtg
     6601 acgtgtaagt gaaattgacc agattgctgt gagtgtaagc gatatgattg gcatccggcg
     6661 accagctaaa ggcgaggccg acttcaaagg tgatcacggc ggtttcgcgc gcgccatcgc
     6721 cgacgatcaa ttgaccgcct tccaaatatt gccgcgcata caataagcgt tgatcgatcg
     6781 gcgaccacat gggcgtttga aacagcgccg gtgtggtatt gatattctga agcgcgttag
     6841 cgcctaaatg atacgtgccg atgaaggccg cgcgggacgt gccgccgacg tgcaacaaca
     6901 acgcatcgct ctgcggggac cacgaataat aatccggctg cccgatcgcg atcgattgca
     6961 ccgtgggcgt cgggctgata gcggtcagtt gcagattcaa actgttcgca gccggatcgg
     7021 attgcagaaa ggcgacatgt tgatcatcgg gcgaaccgta taaataaaac ggcacgcgcg
     7081 cgcgctcggc cgcatacaag atcgtttcgt cagaatcatc gggggctacg ctgatcaatt
     7141 ggctgcccgt ggccgtggtc gccgcgtaga ttagtcgggt tccagcccgt gaccaaatca
     7201 gcgccccggt tggcacacgg ccatcgaccg tgatcgatgc ttgatttccg ccatccggat
     7261 cgatcgtata gatctgatct tgatcgatca ccgcgatccg gttggtggcg gagccgatcg
     7321 attgaactgt ctcgatcggc tgcgtcgcga ctggatgggg ttgattgaat gcagcgacgc
     7381 cccagacgat taccgcgatc aagccgccga tgatcaagat caagccgacg atgaagacga
     7441 tgagcgggct gcgcgttgaa gaagaaggtt gcatggcgag attataccgc gaatggaaga
     7501 cgattgagag aaagcgagat agcgagctgg agagatggag agattgcgag atggagagag
     7561 accacgagcg atatgctatt tgctaaatga aagtcgctct gctataatct cgatcgacat
     7621 tcggggcgtg gcgcagcccg gctagcgcac ctgcatgggg tgc
//
LOCUS       DASRTJ010000283         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_347695,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000283 DASRTJ010000000
VERSION     DASRTJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347695"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /locus_tag="VFF70_05795"
     CDS             complement(<1..2059)
                     /locus_tag="VFF70_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZY44248.1"
                     /translation="MISTKNLLKPASGEPVVGPAKDMVLGVYYLTMMKEGLQGEGRIF
                     GSLEEVELAYSLGQLHLHTKIKLNTETVYGLNSNERFADNRSRVRLIDTTVGRAIFNT
                     IVPNELRFVNDVLDRVKLNDLVARCYQWLGLEPTTELVDAIKNIGFKYATRSGTSIAV
                     SDITVPEAKYAIVNETEAKVNEVERQYRRGLLTEEEQYNRMIELWQQATGLVADAVRK
                     ALPPTANLSVMAMSGATKGGFSPIAQLAGMRGLMADPSGRIIALPIRSNFREGLTALE
                     YFISTHGARKGLADTALRTADAGYLTRRLVDVAQDVMINERDCGTESGIWIRRKDDVG
                     GQNMAVRMIGRVPAAPILHPKTGEVVAERNVIIDEDHSEAIDKSGVAEVFMRSPMTCV
                     LRHGICQMCYGRDLGRGTIVALGSAVGIVAAQSIGEPGTQLTLRTFHTGGVAAGGDIT
                     HGLPRVEELFEARKKPRGEAVMSDIDGTVQIVRTESGMRLVKVKSTQMLKDEYEVPGN
                     WALKVENGQEVNEGQEIATRGDQAMAAKHTGKVSIRKNKPLAVVYEVVDEREYEVPAT
                     ARMLVEDGKEVAAGMQMSEGALNPHRILRILGRDATQLYLLQEVQKVYRSQGVNINDK
                     HYEVIIRKMMNRVQIVSSGDADLLPGELVDRLVLQDSNDKLLAEGKRGATAVPVHSCC
                     ATLA"
BASE COUNT          439 a          616 c          525 g          491 t
ORIGIN      
        1 aggcaagcgt ggcgcaacag ctgtgcaccg gcacagctgt tgcgccacgc ttgccttcag
       61 cgagcaactt gtcatttgaa tcctgcagca ccaaccgatc gactaactca cccggcagca
      121 gatcggcatc gccactggac acgatctgca cgcgattcat catcttacgt atgatcactt
      181 cgtaatgctt atcgttgatg ttaacgccct gcgagcgata aactttctgc acttcttgca
      241 acagatacaa ctgggtggca tcgcggccca agatacgcag gatgcgatgt ggattcaacg
      301 cgccttcaga catctgcatg cccgcggcaa cttctttgcc gtcttccacc aacatgcgcg
      361 cagtagccgg cacttcatat tcacgctcgt caaccacctc ataaacaacg gcgagtggtt
      421 tattcttgcg aatggagact ttgccggtat gcttcgccgc catggcttga tcgccgcgtg
      481 tggcgatttc ctggccttcg ttcacttcct ggccattctc aactttcagc gcccaattgc
      541 cgggaacttc atattcgtcc tttagcattt gcgtcgactt cacctttacc aagcgcatgc
      601 cactttcagt acggacgatc tgtacggtgc catcgatatc tgacataacg gcttcaccac
      661 gcggtttctt gcgtgcctcg aatagttctt cgacgcgtgg caggccgtga gtgatgtcgc
      721 cacctgccgc gacaccaccg gtatggaatg ttcgcagagt caactgtgtg cccggctcgc
      781 caatcgattg agcagcgacg ataccaacgg ctgatcccag cgccacgatc gtgccacggc
      841 ccagatcgcg tccgtagcac atttggcaga tgccatgccg caggacacac gtcatgggcg
      901 atcgcatgaa tacttccgcg acgcccgatt tatcgatcgc ttcggaatga tcttcatcaa
      961 tgatcacatt gcgctcggct acaacttcgc cggtcttggg atgcagaata ggcgctgcgg
     1021 gcacacggcc gatcatgcgc acggccatat tttgtccgcc gacgtcgtct ttgcggcgaa
     1081 tccaaatacc gctttcagtc ccgcaatcgc gttcattgat catgacatcc tgggccacgt
     1141 caaccaatcg gcgggtcaga taaccggcat cggctgttcg cagcgcagta tcggctaagc
     1201 ccttgcgcgc accgtgcgtc gagataaagt attcgagtgc cgtcaggcct tcacggaagt
     1261 tagatcgaat tggcagcgcg atgatacggc ctgatggatc agccatcaag ccgcgcatac
     1321 cggccaattg cgcaatgggc gagaagccgc ctttcgttgc gcccgacatt gccatcacac
     1381 tcaagtttgc cgtcggcggc agcgctttgc gaaccgcgtc ggcgaccaag cctgtggctt
     1441 gctgccacaa ttcgatcatt cgattgtatt gctcttcttc ggtgagcagc ccgcgtcgat
     1501 attgtcgctc gacttcattg accttcgctt cggtttcatt cacaatcgcg tacttcgctt
     1561 ccggcacggt gatgtcggac acggcgatcg atgtgcccga ccgagtcgca tatttgaaac
     1621 cgatgttctt aatcgcatcg accaactcgg tcgtgggctc gagaccaagc cattgatagc
     1681 agcgcgctac caagtcattg agcttgacgc gatcaagcac gtcattaaca aagcgaagct
     1741 cattcgggac aatcgtgttg aagattgcgc ggccaacggt cgtgtcgatt aggcgaacgc
     1801 gagagcggtt atccgcaaaa cgctcgttgc tatttaaacc ataaacggtt tcggtgttca
     1861 gtttaatctt tgtgtgaaga tgaagctgtc ccagcgaata ggccaactcg acttcttcca
     1921 aactgccgaa gatgcgacct tcgccctgga gaccttcctt catcatggtc aagtagtaaa
     1981 ctcccagcac catatccttg gccggcccga cgactggttc acccgatgcg ggcttgagca
     2041 ggttcttggt agagatcatt agctcacgtg c
//
LOCUS       DASRTJ010000284         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_16634,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000284 DASRTJ010000000
VERSION     DASRTJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16634"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFF70_05800"
     CDS             <1..228
                     /locus_tag="VFF70_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZY44249.1"
                     /translation="TLVRPSKNIADVTVSSVKKKWKDKAFAAGVDRTEIEHAAVALGV
                     PLDEHIDVVLKAMQSIASTLGLDGSLATTAS"
     gene            404..1633
                     /locus_tag="VFF70_05805"
     CDS             404..1633
                     /locus_tag="VFF70_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY44250.1"
                     /translation="MKLRRFGAVPENQPIEKETQRTCPNCGSLVSTRAKTCVHCGEDL
                     AALAAKEQRAQQLAQQEQSEEAAQRPVRAIAIGVTVIIALIIIGIVIQSSREAAILAL
                     TPTVTRTPTRTPTTTITPTPTPTSAATSTPIPPFKYTVKSGDTPGAIADFYDLGVDEL
                     MAYNKRSVGDFIVVGETLLIPVPTPKPTGTATPIGFVASPTGPAVTPSEIVYTVKSGD
                     TLGGIALSVGVPIDVIQRRNNIEDPESIQIGQQLIIPTGPTPTFTPAPIPAEATATPR
                     PTYAPVAALSPLDGEIIIGNSPPILLEWLSSGLLREDEYYRAEVAQVQGLLTQSIRTR
                     ATSWHVPIELFPTETDPNRLFKWKVEIIRQVGIGSDGQPVFAVVSTTNERVFEWLVVA
                     PTPKPTATRLPGSNVTP"
     gene            1856..3067
                     /locus_tag="VFF70_05810"
     CDS             1856..3067
                     /locus_tag="VFF70_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZY44251.1"
                     /translation="MDLQTPYQPYPSYPSQPPRRGLGFRGAVLILIAGMILGAIGGGV
                     AGGLVAVNAIHQAAPASVAASSSASNSVPIVSAATNSLVTAGDELVAAVKKVSPAVVT
                     VVNTMPNQQSYDFFGFSQTTPTATGSGVIISPDGYIVTNNHVIDGYQSLQVIYADGRT
                     VDAKLIGTDQYADLAVIKVSGNVPAVAQLGDSDNLQIGESVIAIGSALGDFKNTVTSG
                     VVSALNRSLDTGNGYSLESLIQTDAAINHGNSGGPLVNLAGQVIGINTAIVRSTGLSG
                     DLAEGLGFTIPSRTVSDITSQLISQGYVDRPFLGIRWQMITPEIAKANSLPMAWGIYI
                     QQVDANTSADQAGLKHGDILTAIGSDNIDGSSAFFNILNRHKVGEVVALTLWRNSSNL
                     TLNVTLQSRPH"
     gene            3254..>3780
                     /locus_tag="VFF70_05815"
     CDS             3254..>3780
                     /locus_tag="VFF70_05815"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HZY44252.1"
                     /translation="MTPPRILLLYATAGAGHRRAAEAVADQLRSRGASVTLLDTVRYA
                     HPIFRAAYIGGGLKLIIRLPRVYDLAYRLSDRSSIDRVLRGPRQRAEQLSARSLLHAI
                     QNVKPDALICTHFLPAELCAGWRRSHQLSAPLVTVITDFEPHRMWLHLGTNGYCVATE
                     EAADRLMLDGIDRSM"
BASE COUNT          827 a         1140 c          976 g          837 t
ORIGIN      
        1 actttagtcc gaccgagcaa gaatattgcc gatgtgactg tctctagcgt aaagaagaag
       61 tggaaagaca aagccttcgc cgccggagtc gatcggactg agatcgaaca cgccgcagtc
      121 gctttgggcg tgccgctcga tgaacacatc gatgtcgtgc tgaaggcgat gcaatcgatc
      181 gcatcgacgc tgggactgga tggaagttta gcaacgacgg cgtcatgacc tcatgtgaaa
      241 ctctttctcg tgtggaaaaa gattcatgcg tccttgcgag gcgcgtcttt ttgcgccgaa
      301 gcaatctcag ctgattgctg gcgagattgc ttcgctgcgc tcgcaatgac agttctatcg
      361 tagtcgattc agaaggttgc acatcgcgtt actatctatt tctatgaaac tccgccgatt
      421 tggagctgta cctgaaaatc aacccatcga aaaagaaacg cagcgcactt gccccaactg
      481 cggctcgctt gtttcgacgc gcgccaaaac gtgtgtccac tgcggcgaag acctcgcggc
      541 actcgcggcc aaagagcagc gcgcccaaca attggctcaa caagaacaaa gtgaagaagc
      601 cgcgcagcgt ccggtgcgcg ctattgcgat cggtgtgacc gtcatcattg cgctgatcat
      661 catcggcatc gttattcaaa gttcacgcga agcggcgatc ttggcgctga cacccacggt
      721 gacccggacc ccgacgcgca cacccacgac caccatcacg ccgacaccca cgccaacctc
      781 tgccgcaacg tcgacaccga ttccgccatt taaatacacc gtgaaatctg gtgatacacc
      841 cggcgccata gccgatttct atgatctcgg tgtggatgag ttgatggctt acaataaaag
      901 aagcgttggc gatttcatcg tcgtcggtga aacattgctg attccggtgc cgacgccgaa
      961 gccgacgggc accgcgacac cgatcgggtt tgtcgccagc cccaccggcc cggccgtcac
     1021 ccccagcgag atcgtttata cggtgaagtc aggcgacact ttgggcggca tcgcgttaag
     1081 tgtgggcgtg ccgatcgatg tgatccagcg gcgcaacaac atcgaagatc cggagagcat
     1141 ccagatcggc cagcagttga tcattccgac cggccccacg cccaccttca cgcccgcgcc
     1201 gattcctgcc gaagcgactg cgactccgcg acccacctac gcgccggtag ctgccttatc
     1261 cccattggat ggcgagatca tcatcggtaa ttcaccgccg atattattag aatggttatc
     1321 atccggcttg ttgcgcgagg atgaatacta ccgcgccgaa gtggcacagg tgcagggctt
     1381 gctcacccaa tcgatccgca cccgcgccac gagctggcat gtgccgatcg agttgtttcc
     1441 aaccgagacg gatccgaatc gcctgttcaa atggaaagtg gaaatcattc gacaggtcgg
     1501 gattggcagt gatgggcagc ccgtcttcgc ggtggtcagc accacgaacg agcgagtctt
     1561 tgaatggcta gtggttgcac cgacgccgaa gcccaccgct acgcgtctgc ccggctcaaa
     1621 tgtgacgcca taacttcttt caagtgacca catacttcat tatttattaa cctcccgctc
     1681 aaccttggcg ggaggttgct cgttctaagc cgcgccactt acttgtttct atcgcccgct
     1741 taacgtccgc ttaagcatcc agcgcgacaa taaacttgtt aatgttgaag atcggaaata
     1801 tgattctaat agaaaaatct tcagcaagtt cacaaatatt tattggtgaa agactatgga
     1861 cctgcagact ccttaccagc catatccatc ttatccatcc caacctcccc ggcgcgggtt
     1921 ggggtttcgc ggcgcagttt tgatcttgat cgcaggcatg atccttggcg cgatcggcgg
     1981 cggcgtggct ggcggcttag tcgccgttaa tgccattcat caagccgcgc ccgcctcggt
     2041 cgcagcgagt tcgtccgcct ccaacagcgt gccgatcgtg tccgcggcaa ctaattctct
     2101 cgtcactgct ggcgacgaat tggttgctgc cgtaaagaaa gtcagcccgg ctgtggtcac
     2161 tgtcgtcaac actatgccga atcagcaatc atatgatttc ttcggcttta gccagaccac
     2221 gcccactgcc acaggctcag gcgtgattat ctcgccggac ggatatatcg tcaccaacaa
     2281 ccacgtcatc gacggctatc aatctctgca agtaatttat gcggatggcc gtacggtcga
     2341 cgcgaaattg atcggcaccg atcaatatgc cgatttggcc gtcatcaaag tcagcggcaa
     2401 tgtgccggct gtggcacagt tgggtgattc ggataacttg cagatcggcg agagcgtcat
     2461 cgctatcggc agtgcactgg gcgatttcaa gaacactgtc acctccggcg tcgtcagcgc
     2521 cctcaatcgc agtctcgata cgggcaacgg ctattcatta gaaagtctga tccaaaccga
     2581 tgccgccatc aatcacggca attccggtgg cccgctcgtc aatctcgctg gtcaagtgat
     2641 cggcatcaat accgctatcg tgcgcagcac cggcctgagc ggcgatctgg ctgaagggct
     2701 gggctttacg attccctccc gcaccgtcag cgacatcacc tcacaattga tcagtcaggg
     2761 ctacgtcgat cgaccgtttc tgggcattcg ctggcaaatg attacgcccg agatcgctaa
     2821 agccaacagc ctgcccatgg cttggggcat ctatatccaa caagttgatg cgaacacctc
     2881 agcagatcaa gccggtctca aacatggtga catcctcacc gccatcggca gcgacaatat
     2941 cgacggcagc tcggcgttct tcaatattct caatcggcat aaagtcggtg aagtcgtcgc
     3001 actcaccctc tggcgtaata gcagcaacct gacgctgaac gtcaccttgc aaagccggcc
     3061 tcattagtca cccttgtttt cagtgaggtt gcacttgaca gatgaggtat aattttgttg
     3121 caactcgccc gttttccttc ctcctttgcc gggcggttgt caggggcact gatccctcga
     3181 tcagtgcccc tggtcttttt atgacctcaa cctctcaccg tcaacgtcca atgcaatcaa
     3241 gtataatcaa cgcgtgacac caccgcggat cctcctgctc tatgccacgg ccggcgccgg
     3301 tcatcgccgg gcagctgaag ctgtggctga tcaattgcga tcgcgcggcg ccagcgtcac
     3361 gctgctcgat actgttcgtt acgcccatcc gatctttcgc gccgcctata tcggcggcgg
     3421 attgaagctg atcatccgct taccgcgagt gtatgatctc gcctatcggt tatccgatcg
     3481 atcttcgatc gatcgcgtgc tgcgcggacc gcgccaacgt gctgagcaac tcagcgcccg
     3541 atcgttgtta cacgcgatcc agaatgtcaa acccgatgcg ctgatctgca cacactttct
     3601 gcccgccgaa ctgtgcgcgg gctggcgacg atcgcatcaa ctctccgcgc cgttggtgac
     3661 cgtcatcacc gacttcgaac cgcatcgcat gtggctgcac ctcggcacca atggctattg
     3721 tgtcgccacc gaagaagccg ccgatcgatt gatgctggac gggatcgatc gatccatgat
//
LOCUS       DASRTJ010000285         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_16832,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000285 DASRTJ010000000
VERSION     DASRTJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16832"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /gene="nifJ"
                     /locus_tag="VFF70_05820"
     CDS             complement(<1..1742)
                     /gene="nifJ"
                     /locus_tag="VFF70_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZY44253.1"
                     /translation="MTRQTITIDGNEAAAYVAYRVSEVIAIYPITPSSAMGEWADAWS
                     ALDTKNIWGTRPLVQEMQSEGGAAGAVHGALQTGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARSIAAQALSIFGDHADVMAARSTGWALLASNSVQEVMDTALIAHAAT
                     LQARIPFIHFFDGFRTSHEVMKIEQLTDDDIRSMIDDDLVRAHRSRMLTPDRPVMRGA
                     AQDPDVYFQGREASNPFYEKVPSIVQATMDKFAKLTGRQYHLFDYYGAPDADRVIVMI
                     GSGAETAQETIDYLNAHGYKAGLIKVRLVRPFSIEHLIAAIPSSAKSIAVLDRTKEPG
                     SAGEPLYQDIVTALAETFASGTLKSMPRVFGGRYGLSSKEFTPAMVKAVYDELAKDKP
                     KNHFTVGIQDDVSHTSLDFDPDFNIEPADVVRALFYGLGSDGTVGANKNSIKIIGEDT
                     PNFAQGYFVYDSKKSGSITTSHLRFGPRPIHSTYLIKSANFVACHQWIFLERYDFLAK
                     AAPGATFLLNSVYNPDDVWAHLPRNAQEHILNKKLKFYVIDGYEVAKQTGMGNRVNTI
                     MQTCFFAISGVLPK"
BASE COUNT          378 a          484 c          514 g          411 t
ORIGIN      
        1 tctttaggca ggacaccgct gatcgcaaag aagcaggtct gcatgatggt gttgacgcga
       61 ttgcccatgc cggtctgctt cgccacttca taaccatcga tgacatagaa tttcaatttc
      121 ttattgagga tatgttcttg agcgttgcgc ggcaagtgtg cccacacgtc gtccgggtta
      181 tagacgctgt tcaataagaa ggtcgcgccc ggcgcagctt tagccagaaa atcatagcgc
      241 tccaaaaaga tccattgatg acacgcgacg aaattcgcgg acttgatcaa gtaagtggag
      301 tggatcgggc gcgggccgaa gcgcagatgc gaagtcgtga tcgagccgga cttctttgaa
      361 tcgtacacga agtatccttg cgcaaaattg ggcgtgtctt cgccgataat cttgatcgag
      421 tttttgtttg cgccgacggt gccgtccgag cctaagccgt agaacagcgc gcgtacgaca
      481 tccgccggtt cgatgttgaa gtctggatcg aaatccaaac tggtatgact gacatcatcc
      541 tggatgccga cggtgaaatg atttttgggt ttgtctttcg ccagttcgtc atagaccgct
      601 ttgaccatag cgggcgtgaa ttctttggag gagagaccat agcgtccgcc gaagacccgc
      661 ggcatcgatt tcaaagtgcc gctggcgaat gtttccgcca acgcggtgac gatatcttga
      721 tacagaggct cgcccgcgct gcccggctct ttggttcgat ccaaaaccgc gatcgatttc
      781 gcgctcgacg ggatcgcggc gatgagatgc tcgatcgaga acggacgaac caagcggact
      841 ttgatcaagc cggctttgta gccgtgcgcg ttcaagtaat cgatcgtttc ctgtgccgtt
      901 tctgcgcccg atccgatcat gacaatcacg cgatccgcgt ccggcgcgcc gtagtaatcg
      961 aacagatgat attgtcgtcc ggtgagtttg gcgaacttat ccatcgtcgc ttgcacgatc
     1021 gacggaactt tttcatagaa gggattggat gcctcgcggc cctgaaagta aacatcgggg
     1081 tcttgggccg cgccgcgcat gacaggccga tcgggtgtta gcatgcgcga tcggtgcgct
     1141 cgcaccagat cgtcatcgat catcgatcga atatcgtcat cggtcagctg ttcgatcttc
     1201 atgacttcgt gtgacgtgcg gaagccatcg aagaaatgaa tgaaggggat gcgcgcttgc
     1261 agcgtggccg catgcgcgat caaggccgtg tccatgactt cttgaacgga attggaggcc
     1321 agcagcgccc agccggtcga tcgggcggcc atcacgtccg cgtgatcgcc gaagatcgac
     1381 agtgcctgcg ccgcgatcga tcgcgccgcg acgtgaaaga ccgtcgaggt caactcgcct
     1441 gcgatcttgt acatgttggg aatcatcaac aacagacctt gtgacgcggt gaaggtcgtc
     1501 gtcaacgatc cggtctgcaa cgcgccgtgc accgcgcccg ccgcgccgcc ttcgctttgc
     1561 atctcttgca ccaacggtcg ggtgccccag atatttttcg tatccagcgc cgaccaggca
     1621 tcggcccatt cgcccatcgc gctggagggc gtaatcgggt aaatggcaat cacttcgctg
     1681 acgcggtagg ccacataagc cgccgcttcg ttaccatcga tcgtaatggt ttgtcgggtc
     1741 atctttacct cagggatcag ggattgggaa ataggtttca agtttca
//
LOCUS       DASRTJ010000286         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_319671,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000286 DASRTJ010000000
VERSION     DASRTJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319671"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFF70_05825"
     CDS             <1..1141
                     /locus_tag="VFF70_05825"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44254.1"
                     /translation="DISAAIKLCVEYINAFGGHPGAAGLSLPVNNISIFRSALSRAVE
                     SIRDKSIVINTLPIDAEITLDRATIDLAQQLDRLAPFGEGNPAITLATRSVTLHHDRK
                     FGRKNEHREVTVTDESGTLRSMIWWRGANADLPQSQFDVAYSIKPTDFRGEPNLSIEW
                     IDFQVTQVPVIEVSAPKIEMEIIDWRSESNLAEVIFSKFADRLKPSHSLAIWSDGIHA
                     APLPIVRRYDLIEAEVLIIWSPPCGWRELSNAIEQVKPKRIYLGAVEASEDRLEPFLK
                     RLAGLLKHDLTQRNGRVEVARLAAALGQRLATTRKGIEWLEDAGQIKVSDWGDSEIII
                     SATGGDKSTEAAEVQSELKVLLAETAAWRAYYRRMDVAQIRALVR"
     gene            1195..1743
                     /locus_tag="VFF70_05830"
     CDS             1195..1743
                     /locus_tag="VFF70_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44255.1"
                     /translation="MPEVTLRPIQDNDRKWIKQFIIDRWGAEIVVVHDMVYRPDELAG
                     FVAAYAVIVGRSFDPVTIKNTAYTVGRDQPIGLITYHIQANQCEIVTLDSLKSGIGIG
                     TALIAAVKASAKQAGCRRIWLMTTNDNLNGLRFYQKRGFVVVAVNRNAIEQSRQIKPE
                     IPLIGAEGIPLRDEIELEYDLA"
     gene            1875..2519
                     /locus_tag="VFF70_05835"
     CDS             1875..2519
                     /locus_tag="VFF70_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZY44256.1"
                     /translation="MLVKERMSRQPILAAPDMTMSEALAQMKKEHVRRMPVIDKHGKL
                     IGIVSDKDLLHASPSPATTLSVWEIPALLAKIKVEDVMTKKVITVTEDTPLEDAARIM
                     VDNKIGGLPVVHGDKVVGIITETDLFKIFLELLGARHKGVRITMLVIDVKGQLAKIAQ
                     AIAAVGGNIIAMGEVQGTDSTNYQVMIKVVDVPQDKLVAALTPYVIKIEDVREV"
     gene            2679..3155
                     /gene="bcp"
                     /locus_tag="VFF70_05840"
     CDS             2679..3155
                     /gene="bcp"
                     /locus_tag="VFF70_05840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZY44257.1"
                     /translation="MATTELRIGDAAPEFEMLTDQGTAVKLSDYRGQRVILYFYPEDD
                     TPGCTTQACGFRDRYVQIEEKNAVVLGVSPDNVASHQRFKTKFNLPFTLLVDQDHKVA
                     ELYGTWAERTSSSGKSHMGIIRSHFVIDENGKLIDVQYTIKAPDSVAKALATIGIK"
     gene            3165..3395
                     /locus_tag="VFF70_05845"
     CDS             3165..3395
                     /locus_tag="VFF70_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZY44258.1"
                     /translation="MCRTIKQLRSSEIEPTDQEIQEAALQFVRKVSGYRKPSKANEAA
                     FNQAVIKIAASTRELLDNLVVGKETAHLQHVH"
BASE COUNT          934 a          903 c          995 g          776 t
ORIGIN      
        1 ggatattagc gcggcgatca aattgtgcgt cgaatacatt aacgccttcg gcggccatcc
       61 cggcgcagcg ggtttgtcac tgcctgtcaa taacatttcc atttttagaa gcgcgctctc
      121 ccgagctgtt gaatcgattc gcgataaatc gatcgtaatc aacacgctgc cgatcgatgc
      181 ggagatcaca ttggatcgag ccacgatcga tctggcccaa cagctcgatc ggttagcccc
      241 tttcggtgag ggcaacccgg cgatcacatt agcgacacgc agcgttacac tccatcacga
      301 tcgaaagttt ggccgcaaga acgaacatcg cgaagtgacg gtgaccgacg agagcggcac
      361 acttcgatca atgatctggt ggcgtggcgc gaacgccgat ctaccgcaaa gccagtttga
      421 tgtggcttac tcgatcaagc cgactgattt tcgcggcgag ccgaacctat cgatcgagtg
      481 gatcgacttt caagttacac aagtgccggt cattgaagtc agcgcaccca agatcgaaat
      541 ggagataatc gattggcgtt ctgagagcaa cctcgcggag gttattttca gcaagtttgc
      601 tgatcgcctg aaaccatcgc acagtttggc aatctggtca gacggcattc acgcagcccc
      661 cctcccaatt gtgcggcgct acgatttgat tgaagccgag gtattaatca tttggtcgcc
      721 gccgtgcggc tggcgcgaac tcagtaacgc aatcgagcaa gtgaagccaa agcggatata
      781 cttgggcgcc gtcgaggcca gcgaagatcg actcgaaccg tttctcaagc ggctggccgg
      841 attattgaag cacgatctca cccaacgcaa tggacgcgtc gaggtggcga gattggccgc
      901 ggcgctcggt caacggcttg cgacaactcg aaaaggtatc gagtggctgg aagatgcggg
      961 gcaaatcaag gtgagcgatt ggggcgacag tgaaattatc atctcagcca ctggtgggga
     1021 taaatctaca gaagcagctg aggttcaatc ggagttgaaa gttttattgg ccgagaccgc
     1081 cgcttggcgt gcatactatc ggcgaatgga tgtggcgcag attagagcgt tggtgagata
     1141 agatcattca gcccaagaag aggcaagtat cctcacgatc acgctgaaac aacgatgcca
     1201 gaggtcacac ttcggccaat tcaagacaat gatcgaaagt ggatcaaaca gtttatcatc
     1261 gatcgctggg gcgcagagat tgttgtagtg catgacatgg tctatcggcc agacgagctg
     1321 gctggctttg tcgcggcata tgcggtaata gtggggaggt ctttcgaccc cgtaaccata
     1381 aagaacacag cgtataccgt cgggcgagat caaccgatcg ggttaataac ttaccacatc
     1441 caagctaatc aatgtgagat tgtgacacta gatagcctta agtcggggat cggaatcggc
     1501 acggcgttga ttgcggctgt taaagctagc gccaaacaag cggggtgcag acgcatctgg
     1561 ttgatgacca cgaatgataa tttgaatggg ctgcgttttt atcaaaagcg cggtttcgtt
     1621 gtcgtcgcag ttaatcgcaa tgctatcgag caatcgcgcc agatcaaacc tgaaattcca
     1681 ctgatcggcg cagagggcat accgctgcgt gacgagatcg agctggaata cgatctcgca
     1741 taatttcacc atcgaataag cctgacatcc acgaataaat atgacaaacg acacagccga
     1801 gcccggtact tctatgctag actgatgtgt acgcgccctc aaagaccgcg cgaattgaag
     1861 acaggagaat gctcatgctt gttaaagaac gaatgtcgcg ccagccgatt ctggctgcgc
     1921 cagatatgac gatgagcgaa gcgctggcgc aaatgaagaa ggagcacgtc cgacgcatgc
     1981 ccgtgatcga taaacacggc aaactgatcg gtattgtatc cgacaaagat ctgctgcacg
     2041 cctcgccctc gccggcaacg accttgagtg tgtgggagat tccggcgctg ctggccaaga
     2101 tcaaggttga agatgtgatg accaagaaag tcatcacggt cactgaagat acgccgctgg
     2161 aagatgcggc gcgcatcatg gtcgataaca agatcggcgg tctgccggtc gtgcacggcg
     2221 acaaagttgt cggtatcatc actgaaaccg atttattcaa gatctttttg gaactgctcg
     2281 gcgcgcgcca caaaggagtc cgcatcacca tgctggtcat cgatgtgaaa ggccaactcg
     2341 ccaagatcgc ccaagcaatt gctgccgtcg gcggcaatat tattgcgatg ggtgaagtgc
     2401 aaggcaccga ttcgacgaat tatcaggtca tgatcaaggt cgtggatgtg cctcaagaca
     2461 aattagtcgc ggccctcacg ccttatgtga ttaagatcga agacgtccgc gaagtttaat
     2521 cgagaagcaa cccggcaaag gattgaccgc ctcccgtcta caatcagacg tgaggcggtc
     2581 gtgtttatcg tcgcatctct caagcggcaa tgctgtagcg agttgccgcg aaatcggtta
     2641 aacttactat caattattaa taggaggggg agctgtgtat ggcgacgact gaattgagaa
     2701 ttggcgatgc ggcgcctgag tttgaaatgc tcaccgacca aggcaccgcg gtaaagttat
     2761 cggactatcg gggtcagcgc gtgatattgt atttctatcc cgaagatgat acaccgggct
     2821 gcacgaccca agcgtgcggt ttccgcgatc gctatgtgca aatcgaagag aaaaacgcgg
     2881 tcgtgttagg cgtcagtccg gataacgtgg cttcccatca acgcttcaaa acgaagttta
     2941 acttgccgtt cactctgtta gtcgatcaag atcacaaggt tgccgagttg tatggcacgt
     3001 gggctgaaag aacctcctcc tctggtaaat cgcatatggg gatcatccgc agtcatttcg
     3061 tgatcgatga gaatggtaag ttgatcgatg tgcagtatac gatcaaagcg ccggacagtg
     3121 tagcgaaggc gttggcaacg atcggtatca agtgaggtga atctatgtgc cgcaccatca
     3181 aacaactccg aagttctgaa atcgaaccga ccgatcaaga aattcaagag gctgcactgc
     3241 aattcgtgcg caaggtcagc ggctatcgta agccgtcgaa agcgaacgaa gcggcgttta
     3301 accaagcggt gatcaagatc gccgcctcga cgcgcgaact actggacaac cttgtcgtcg
     3361 gtaaagaaac agcgcacttg caacacgtcc actaaaagca ttggagatca attgaatgtc
     3421 atatcgacct gggggcacac catgaaactc aaccgttatc tttcacgcag cgtggccatt
     3481 ctggtgcttt atttcggcgt cagcacaatc gcgggattgc gcagtgaagc aagtcccgcc
     3541 gcgccgacct tcaccgtcaa cagtttgggc gacgcgccgg ccgacttcac tggtgatccc
     3601 ggctacac
//
LOCUS       DASRTJ010000287         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_347788,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000287 DASRTJ010000000
VERSION     DASRTJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347788"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1317)
                     /gene="hutI"
                     /locus_tag="VFF70_05850"
     CDS             complement(55..1317)
                     /gene="hutI"
                     /locus_tag="VFF70_05850"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZY44259.1"
                     /translation="MNVDLLITNASQLLTIPAHNDGPQRGTHLGDIGSIEDGALAING
                     ESIVDRGSSADLIKRHQAKQTIDANQQLVMPGFVDPHTHVVYAGDRANEFEQRIAGAS
                     YMEIMNAGGGIMSTVRSTRAASIDQLIDQTRLRLDRMLMHGTTTIEAKTGYGLETQAE
                     LKMLDAILQLDKQHAIDLVPTFLGAHAIPIEYKGREDAYTDLVVDEMLPALSESKPEF
                     VDVFCEAGAFTLAQTRKILIRAKELGYALKIHVDEFEPLGGTRLGVELGAVSVDHVVS
                     TPQSEVDLLGQSKTIAVGLPGTPFGLAQKDYTPAKAILEAGGALSIATDLNPGTSWCE
                     SMQMVIALACRYMKLAQAQALAAATINAAFAIGRGESIGSIEIGKQADVLIMDVGDYP
                     SIGYRYGTNLVKSVIKRGKIVYADGRLL"
     gene            complement(1380..1456)
                     /locus_tag="VFF70_05855"
     tRNA            complement(1380..1456)
                     /locus_tag="VFF70_05855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Arg,seq:cct)
     gene            complement(1524..2537)
                     /locus_tag="VFF70_05860"
     CDS             complement(1524..2537)
                     /locus_tag="VFF70_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY44260.1"
                     /translation="MVVVMIAVTGATGFLGHNLIPLLVERGHTVRILARSTSQHQFLI
                     DLGVEEIHGDTRDASAVDRLMDGCDAVIHGAGYFRFWGKADDFHSTNVLGTANVLEAA
                     HRSNVQRFIHISSVVVVGAPKPGTIIDEQYVCEPRDDYQRSKLAGEALVTNYHDNFGL
                     PTIIFRPGAFYGPYGRYAWNRLFFEDPHIHHLPIQIHGGQLLTFPIYLPDLARVIDAA
                     LTRGTPGEIYNVSGPSMTHHEVNDVISRLIGDHPHFFNAPDWGMLLIARLLTFFSRFT
                     HHEPYYPINLAPYVFNNWIVDSSKAVRDLGFEATPFVDGARATIEWYRQSGLLRKRGE
                     FNS"
     gene            complement(2613..>3233)
                     /locus_tag="VFF70_05865"
     CDS             complement(2613..>3233)
                     /locus_tag="VFF70_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HZY44261.1"
                     /translation="VEELAVQVNNLEVAYHDPRAMASMMLTYATSPRGACHNQSDVFL
                     AEIGTEHPEWNIPMLQDPLSEEGKAQIAAQHQNYRSVDNSLVLCFFAHVPGLTALSLL
                     NAAVGLDWTMEDLQRAGERIWNLKRAINNRFGLTRANDKLPKLLLEPLPDGPIAGHVP
                     NFDRLLSDYYQVRGWDEQSGKPKRNKLGELGLDFVIETLYPQMTND"
BASE COUNT          717 a          914 c          865 g          737 t
ORIGIN      
        1 ccgatgtatc ggccttcacc gtcagcacag tcttcgtcat gatgtcgcgg actttcatag
       61 taatctcccg tctgcgtaaa cgatttttcc ccgcttgatc acacttttga ccaaattagt
      121 tccatagcga taaccgatcg aaggataatc gccgacgtcc atgatcaaca catcggcttg
      181 cttgccaatc tcgattgacc cgatcgattc accgcgcccg atcgcgaagg cggcgttgat
      241 cgtggccgcc gctaaggcct gcgcctgcgc cagtttcata tagcggcagg ccaaggcgat
      301 caccatttgc atcgactcac accaactggt gccgggattc aagtccgtgg cgatcgataa
      361 cgcgccgccg gcttcgagga tcgctttggc gggcgtgtaa tctttttgcg cgaggccaaa
      421 gggcgtgccg ggtaacccca ccgcgatcgt tttactctgg cccaacaaat ctacttcaga
      481 ttgcggcgtg gacaccacat gatccacgct caccgcgccg agctcaactc cgaggcgggt
      541 gccacccaac ggctcgaatt catcaacgtg aattttaagc gcataaccta gctctttcgc
      601 gcgaattaaa atttttctgg tttgcgccag cgtgaacgcg cccgcttcac aaaagacatc
      661 gacgaactcc ggcttcgatt ctgaaagtgc cggcagcatt tcatcgacga ccaaatctgt
      721 gtaagcatct tcgcggcctt tgtattcgat gggaattgca tgcgcgccga ggaaagtcgg
      781 aactaaatca atggcgtgct gcttatccag ttgcaagatc gcgtcgagca ttttcaactc
      841 tgcttgtgtc tcaagtccat aacccgtctt ggcttcgatc gtggtcgtgc cgtgcatcaa
      901 catccgatca agccgcaatc gagtttgatc gatcagctga tcgatgctgg ccgcgcgcgt
      961 gctgcgcacc gtcgacatga tgccgccgcc cgcattcatg atctccatgt agcttgcgcc
     1021 ggcgatgcgc tgctcaaact cattcgctcg atcgcccgca tacaccacat gcgtgtgcgg
     1081 atcgacaaag ccgggcatga ccagctgctg attcgcgtcg atcgtttgct tggcctgatg
     1141 ccgcttaatg agatcagccg atgagccgcg atcgacgatc gattcgccgt tgatcgccag
     1201 cgcgccgtct tcgatcgatc cgatatcgcc taaatgagtc ccgcgttgag gcccgtcgtt
     1261 gtgagcagga atggtgagga gttgtgaagc gttggtgatg aggagatcga cgttcatgcg
     1321 tcgatcatag catagaaaat aaaaccttgc gaaggattca gcccttgcaa ggttaagcgt
     1381 ggcgcccccg gcaggaattg aacccacaac cctttcctta ggacggaact gctctatcca
     1441 tttgagctac gggggcgatg acggtattat aacatagtgc ggatttgtta taatgatcat
     1501 gctgtttaaa tcgtcagttg cacttacgag ttgaattcac ctcttttcct cagcagccca
     1561 ctttgccgat accactcgat cgtcgcgcgt gcaccatcga caaacggtgt cgcctcaaag
     1621 ccgagatcgc gcacggcttt gctgctgtcg acgatccagt tgttgaagac atagggcgcc
     1681 agattgatgg gatagtaagg ttcgtgatgc gtgaagcgcg agaagaaggt caacagccgg
     1741 gcaatcagca gcatacccca atcgggcgcg ttgaagaaat gcgggtggtc gccgatcagt
     1801 ctactgatca catcattgac ttcatgatgc gtcatgctcg gcccggacac gttgtagatt
     1861 tcgccgggcg tgccgcgtgt caaagccgca tcgatcacgc gcgcgagatc gggcagataa
     1921 atcgggaagg tcagcagctg tccgccatga atctggatcg gcaggtgatg aatatgaggg
     1981 tcttcaaaga acaggcgatt ccaggcatag cgcccgtagg ggccgtaaaa cgcgccggga
     2041 cgaaaaatga tcgtcggcag tccgaagttg tcatggtagt tcgtcaccaa cgcttcgccg
     2101 gccaacttgc tgcgttgata atcatcgcgc ggctcgcaca catactgctc atcgatgatc
     2161 gtgccaggct tcggcgcgcc gacgaccacc acgctggaaa tgtggatgaa gcgctgcacg
     2221 ttcgatcgat gcgcagcttc caatacattt gcagtgccca gcacgttggt gctatgaaaa
     2281 tcatcagcct tgccccaaaa ccgaaaatag cccgcgccgt gaatcaccgc atcgcaaccg
     2341 tccatcagcc gatcgaccgc cgacgcatcg cgcgtgtcgc cgtggatctc ttcgacgccg
     2401 agatcaatga ggaattgatg ttgactcgtg gaccgcgcca gaatgcgaac ggtatgtccg
     2461 cgctctacca agaggggaat gagattgtgg ccgaggaaac cggtcgcgcc ggtgactgca
     2521 atcataacaa cgaccaatcc cactccgctg acgctgcggg gacttcgtga tcaatgaaca
     2581 ctgaacaatc gcaaattcga ctgcgcgccc tgttagtcat ttgtcatttg aggatacagc
     2641 gtttcaataa caaaatctaa gcctaactca cccaatttgt tgcgtttcgg tttgcccgat
     2701 tgttcgtccc agccccgcac ttgatagtag tcgctcaaca atcgatcgaa gtttgggacg
     2761 tggccggcga tcggaccatc gggcagtggc tccagcaaga gtttaggcag tttatcattg
     2821 gcgcgcgtta agccgaagcg attgttgatt gcgcgtttca aattccagat gcgttcgcct
     2881 gcgcgctgca aatcttccat tgtccaatcg agcccgacgg cggcattcag taaactcaat
     2941 gcggtcaatc cgggtacgtg cgcgaagaag cacagcacca gactgttatc gacgctgcga
     3001 tagttctggt gctgcgccgc gatttgcgct ttgccttctt cgctcaacgg gtcttgcagc
     3061 atgggaatgt tccattcagg atgctcggtg ccgatctcgg ccaggaacac atcactctga
     3121 ttgtggcacg caccgcgcgg cgaagtggcg taagtcagca tcatacttgc catcgcacgc
     3181 ggatcgtgat aagccacttc cagattgttg acctgcacgg cgagttcttc cac
//
LOCUS       DASRTJ010000288         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_272298,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000288 DASRTJ010000000
VERSION     DASRTJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272298"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VFF70_05870"
     CDS             complement(<1..1579)
                     /locus_tag="VFF70_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZY44262.1"
                     /translation="MLKRLFVLISIFLAACGNSNTATPIIAPSPTAPAPDTTAQEFLA
                     DWEHADYAAMFKLISSTSQAALTPDTFANRYTAAQSTASVKFIRAEARSALRAADTVH
                     IGYHVEWDTVLFGTLKADSEMVLTLENNQWRITWNDGLIWPDLEGGDAFQIQYSIPRR
                     ANIYDRDGKGLAVDGKIVTICLVPGQIQDEIKLLNGLSPIVNLSPDEIKAKYANAKPD
                     WYVPIADVPFDVSQAHQDVLSLPGFDLRSKAVRVYDGLAPHTIGYVSLIAPEDVQKWK
                     DLGYRGDEWVGASGLEQWGEPYLAGTHGATLSIISAKGDAIKVIAKQAAVPSRSIYTT
                     LNRTLQEATDSILAGHKGAIVVLDPNNGQVLAMSSYPDYNPNALITANVIDVPAETSF
                     LNRATQGAYPPGSTFKPVTMAAGLEKGGLVPTSPFYDPGYWDGLGAGYRKVCWLASGH
                     GNIDLQTGLSASCDVVFYQVGKKVDDIDPDILPTFARSFGLGSTTGITGLNEIAGLVP
                     DPAWKQQTQNDPWRTGDA"
     gene            complement(1793..2524)
                     /locus_tag="VFF70_05875"
     CDS             complement(1793..2524)
                     /locus_tag="VFF70_05875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZY44263.1"
                     /translation="MIAQPDRLVNARILLASASPRRRELITLLSLPFETTSADIDETP
                     LDHELASDLAMRLSQAKAQQVYSHLHWNAWSAVQVSPTLQPSALIVILASDTIVSLDG
                     KPLGKPRDAVEARSMLRRLRDRSHQVFTSITMIDTQHDQTITDLASTDVPMRNYSDEE
                     IEMYITSGDPFDKAGAYAIQHHGFHPADRLTGCYANVMGLPLCHVTRTLQQLGVHPPI
                     NVPVACQTHIQYECPVFEKILMRDE"
     gene            2568..3308
                     /locus_tag="VFF70_05880"
     CDS             2568..3308
                     /locus_tag="VFF70_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HZY44264.1"
                     /translation="MAVRRMAIFIAVNAIVSAIVVLVVLSLWDAGRSSIQPLPTIVPK
                     PIAQTTFQPIQPIAVTATPTVPRSYIVQPGDTPFSIATDLGISVNDLLSANHLIEGDI
                     LNIGQELIVPAAGFTPDVATATPLSVSTTRPNPTPAATSAGGLLVTIRQIDSLGDVVN
                     ESVVLTNLGGKVNLSGWTLSDGEGHKYTFPDLTLLTNAEAHVHTTVGPDSAVDLYWGQ
                     ASAMWNGSGTIAYLRDPSGKLMATYRVP"
BASE COUNT          784 a          886 c          966 g          828 t
ORIGIN      
        1 ccgcatcgcc tgtgcgccac ggatcatttt gggtttgctg tttccaagcg ggatccggca
       61 ccaggccagc aatttcattc aggccggtga taccggttgt tgatccaaga ccaaagctgc
      121 gcgcgaaggt tggcagaata tcgggatcga tgtcgtcgac cttcttgcca acttgataga
      181 agaccacatc gcatgaggcg cttaacccgg tttgcagatc gatgttgcca tgacccgaag
      241 cgagccaaca cactttgcga taacctgcgc caagaccatc ccaatatccc ggatcataaa
      301 atggcgatgt cggtacaagg ccaccttttt ccaagccggc ggccattgtt accggcttga
      361 acgtggagcc aggtggatac gcgccttgcg tcgcgcgatt gaggaacgaa gtctcagcgg
      421 gaacatcgat cacattggca gtgatcagcg cgttgggatt gtagtcggga taactagaca
      481 tggctaagac ttggccgttg ttcggatcga gtacaactat cgcgcctttg tgaccggcca
      541 agatggaatc agtggcttct tgcaatgtgc gattcaaggt ggtgtagatg ctgcgactgg
      601 gcacagcagc ttgtttggcg attactttga tcgcatcacc tttagctgag atgatcgata
      661 atgtggcgcc gtgcgttcca gccaagtatg gctcccccca ttgttcgagc ccactggcgc
      721 cgacccactc atcgccgcga taacccaggt ctttccattt ctgcacatct tccggcgcga
      781 tcaggctgac atagccgatc gtgtgtgggg ctaagccgtc atacacccgc accgctttac
      841 tccgcagatc gaaaccgggc agcgataaaa catcttgatg ggcttgactg acatcgaagg
      901 gcacgtcggc aatcggcaca taccaatcgg gtttggcgtt cgcatacttt gctttgatct
      961 cgtcggggct gagattgacg atcgggctaa ggccattcaa caatttgatc tcatcttgaa
     1021 tttgtccagg cacaagacaa attgtcacaa tcttgccatc gaccgctaag cctttgccat
     1081 cgcgatcgta aatattcgcg cggcgcggaa tggaatattg aatttgaaat gcgtcacccc
     1141 cttcgaggtc gggccagatc aagccgtcgt tccaggtgat gcgccattga ttattctcca
     1201 gcgtcaaaac catttcactg tcggctttca aggtgccgaa taacacagta tcccattcga
     1261 cgtgataacc gatatggacc gtatcggcag cgcgcaaagc cgatcgagct tcggcgcgaa
     1321 tgaacttcac actcgccgtc gattgtgcgg cggtgtagcg attggcaaat gtgtcgggcg
     1381 tgagtgcggc ttgcgaggtg gagctgatca acttgaacat tgcggcataa tcggcgtgtt
     1441 cccaatcggc caaaaattct tgcgccgttg tatcgggtgc gggcgcggtg gggctgggcg
     1501 cgatgatcgg tgtggctgtg ttcgaattgc cgcaagcggc gaggaagatg ctgatcagaa
     1561 cgaagagacg ctttaacatg tgatcattat acgttggcag tcgaaatgtg aaaagtgaag
     1621 agtgatgcat ggcgagtaat gagtaacgag taaatcacgt tgcgtaccgt cattgcgagc
     1681 ggagcgaagc aatctcgcgg gcggtcgtgg agattgcttc gtcgcaaaaa acgctcctcg
     1741 ccatgacgcc tgaaccgtat agggtgagtg agtaaatcac gccatcacgt tatcactcat
     1801 cccgcatcag gattttttca aataccggac actcatactg aatgtgtgtt tgacaagcaa
     1861 ctggaacatt tatcggcgga tggacgccaa gttgctgcaa ggttcgagtc acatgacaca
     1921 atggcagacc catcacattg gcatagcagc ccgtcaatcg atcggccggg tggaagccgt
     1981 gatgttgaat ggcatacgca ccggctttgt cgaacggatc gccgctggtg atgtacattt
     2041 caatttcttc atcagaatag ttacgcatgg gcacatccgt cgaagcgaga tcagtaatcg
     2101 tttgatcgtg ttgggtatcg atcatagtga tcgaggtaaa cacttgatgc gatcgatcgc
     2161 gaagccgcct caacatcgat cgggcctcaa ccgcatcgcg cggcttgccc aacggcttac
     2221 catcgagcga gacgatcgta tcagaagcga gaatgactat caaggctgag ggttgaagag
     2281 tgggggacac ttggaccgca ctccacgcgt tccagtgcag gtgtgaataa acttgttgag
     2341 ctttggcttg actcagacgc atggcgagat cagatgcgag ttcgtgatcg agcggtgtct
     2401 catcaatatc agctgaggtt gtttcaaacg gcaggctgag cagcgtgatt aactcacggc
     2461 ggcgcggtga ggcagacgcg aggagaattc gtgcgttgac gagtcgatct ggctgtgcta
     2521 tcatcgtgaa gtcgttgcag caattataac ggcaagtgag gaatcacatg gctgttcggc
     2581 gtatggcgat ctttattgca gttaacgcga ttgtatcggc gatcgttgtg ctggtcgttt
     2641 taagtctatg ggacgcaggc cgatcgtcga tccagccgtt gcccacaatc gtgccgaaac
     2701 cgatcgcgca gaccactttc caaccgatcc agccgatcgc ggtcacagcc acgccgaccg
     2761 ttccacgcag ctatatcgtt caaccgggtg atacgccgtt cagtattgcc accgacctcg
     2821 gaatctcggt caatgatcta ttaagtgcca atcatttgat agagggcgac attctcaaca
     2881 tcggtcaaga gctgatcgtt ccggctgctg gtttcacgcc ggatgttgcg acagctactc
     2941 cactcagcgt atcgaccact cgacctaatc ccactccagc ggcaacatcg gctggcggct
     3001 tgctcgtcac gatccgtcag attgatagtt tgggtgatgt ggtgaatgaa tcggttgtct
     3061 taactaacct cggcggcaaa gtcaatcttt ccggctggac cttatcggat ggcgaggggc
     3121 acaaatatac tttccccgat ctcacattgc tgacgaatgc tgaagcgcat gttcatacga
     3181 cggtcggccc agacagcgcc gtagatttgt attggggaca agcgagcgcg atgtggaatg
     3241 gcagcgggac gatcgcctac ttgcgcgatc cgagtggaaa gttgatggca acttatcgtg
     3301 tgccttagcc agcgttcaat aacaaaacag ccacgcgggt gagtggctgt ttttattttc
     3361 gatcagctct tcttctgttc atccgttggc ggcgtcggcg ggactttctt ttcgtcttcg
     3421 cctgtcagtc ccttgcggaa ctcgcgcatg ccggtgccta attc
//
LOCUS       DASRTJ010000289         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_16968,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000289 DASRTJ010000000
VERSION     DASRTJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16968"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1230
                     /locus_tag="VFF70_05885"
     CDS             109..1230
                     /locus_tag="VFF70_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HZY44265.1"
                     /translation="MPELQPPSLITTAAQLEAAIDRISQHTSIAVDTESDSLYVYREK
                     VCLIQISIPENDFLIDPLAGFDLQLLGHLFANPKVEAIFHAAEYDVICLRRDYGFTFS
                     QLYDTMWVARILGWPRVGLGDILKEKYGIQLDKRWQRHNWGKRPLEKEALAYARFDTH
                     YLDQLREAQLVELHQTDRLAEAQEVFAELTYSSIDPRAFDPDDFWHVKGVWDLRGHAL
                     SIVRRLYALRDREAKRQNRPPFKIMGDRTLTALAQTAPHNLQQLEAIDGLSPLLIKRY
                     GRSILEAIDHGRKDAVPTAPRRMPPDQVVLARYEKLRAWRKQAAADRGVEPDVIVGNA
                     VLMEIARRQPHALIDLENIEGLGPWRRGAYGQALLNVIA"
     gene            1284..2681
                     /locus_tag="VFF70_05890"
     CDS             1284..2681
                     /locus_tag="VFF70_05890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44266.1"
                     /translation="MQIQFWGAARTVTGSMHLLTINGQKLLLDCGLFQGKRSEANERN
                     GHLPFDAKSIDACILSHAHMDHSGVLPVLTKLGYNGHIFCTSATRDLSTLMLHDSAHI
                     QESDALFFNKHAAERSLLPIKPIYTEADARETVQHFVVYEYHRPFEPIPGVQATFFDA
                     GHILGSTFITLDITEGAKKFRLVFTGDLGRKRLPILRDPESADRADIYITEGTYGGRH
                     HAPIEDMRNELAQIVKDTADRKGVIVIPAFAVGRTQEIVYDLQQLVDQKQIPKLPIYV
                     DSPLAINVTEVFRLHPECYDEDINSFIKSDPDGDAFGFSQLTYVRESAASKALNDRTD
                     PMIIIAASGMCEAGRVLHHLRHHLGDPRSTIIFAGYQAENTLGRKILDGQPHVRIFGE
                     EHVVKAQIKKLEGYSAHGDHDDLIGFAHNMAKKPSRTFIVHAEYEAAQALQTGLTGVG
                     LQNIAIPNRGDLVEI"
     gene            2833..2924
                     /locus_tag="VFF70_05895"
     tRNA            2833..2924
                     /locus_tag="VFF70_05895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2867..2869,aa:Ser,seq:cga)
     gene            2977..3495
                     /locus_tag="VFF70_05900"
     CDS             2977..3495
                     /locus_tag="VFF70_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZY44267.1"
                     /translation="MSSLTEFRRQKDVFFKTDPQSPLMPDQRTNFDSLRYFPENPQLR
                     ITTKVEESSDQQIVPMITSTGAVKEYYKYGQFSFEVDGQTATLQVYQDADRTYFFLPF
                     VDATAPEETYGAGRYLDLEPEHGNTFVIDFNYAYNPYCAYNENWNCPIPPRENRLAVR
                     IEAGEKTFKKDL"
     gene            complement(3569..>4021)
                     /locus_tag="VFF70_05905"
     CDS             complement(3569..>4021)
                     /locus_tag="VFF70_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY44268.1"
                     /translation="VHGHALGGGCELALCCDLIVAAEGANFGQPEIKLATIAPIAALR
                     LPVLIGSRRAAELLFTGEPIGSIEAAQIGLINRAVPIDQLDRTVDQLIGKLINLSGAA
                     LRLCKRAMRVGALDTMEKLYLDELMSTEDAHEGLAAFMEKRAPIWKHR"
BASE COUNT          969 a         1147 c         1013 g          892 t
ORIGIN      
        1 tcggatgatc atgtcaaatc attttgaaga ttcgcctcgc gatcattctt gactttgatc
       61 gcactcgcct cgataatagg ccgcagacca cgaacacggc caaccatcat gcccgaactt
      121 caaccaccca gtttgatcac caccgctgct caacttgaag cagcgatcga tcggatcagc
      181 cagcacacct cgatcgccgt ggataccgaa tccgatagct tgtatgtcta tcgcgaaaaa
      241 gtctgcttga tccaaatctc cattccggaa aatgatttct tgatcgatcc gctcgccgga
      301 tttgatctgc agctactggg tcacttgttt gccaatccca aagtcgaagc gatcttccac
      361 gcggcggaat acgatgtgat ttgtctgcgg cgcgattacg gtttcacttt ttcccagttg
      421 tatgacacga tgtgggtcgc gcgcattctg ggttggccgc gcgtgggatt gggcgacatc
      481 ttgaaagaaa agtacggcat tcaattggac aagcgttggc agcgccataa ttggggcaag
      541 cgaccgcttg agaaagaggc gttggcttat gcgcgctttg atacgcacta tctcgatcaa
      601 ctgcgtgagg cgcagctcgt cgagctgcac caaaccgatc ggttggccga agcccaggaa
      661 gttttcgccg aactcaccta ctcgtcgatc gatccgcgcg cgttcgatcc cgatgacttc
      721 tggcacgtaa aaggcgtgtg ggatttaaga ggtcatgcgc tgtcaatcgt gcgccgattg
      781 tatgcgctgc gcgatcgcga agccaaacgc caaaaccggc cgccgttcaa aattatgggc
      841 gatcgaacgt tgacggcttt ggctcagacc gcgccgcaca acctgcaaca actggaagcg
      901 atcgacggtt taagtccgct gttgatcaaa cgctacgggc gatcgatctt ggaagccatc
      961 gatcatggcc gcaaggacgc agtgcccacg gccccgcgcc gcatgccgcc cgatcaagtt
     1021 gtgttggcgc gctacgaaaa attacgcgcg tggcgcaaac aagcggcagc cgatcgcggt
     1081 gtcgagccgg atgtgattgt gggcaatgcc gtcttgatgg aaatagcgcg ccgccaaccg
     1141 catgcgttga tcgatcttga aaacattgaa ggcttaggtc cgtggcggcg cggcgcttat
     1201 ggtcaggcct tgctgaacgt gatcgcgtaa tcacgcgatc acggattaac tttgttgttt
     1261 actttttgaa ggagggcggc agcatgcaaa ttcaattctg gggcgcagcg cgtaccgtga
     1321 ctggttcaat gcatctcttg accatcaacg gccagaaact attgctcgac tgcgggctat
     1381 ttcaaggcaa acgatccgaa gcgaacgaac gcaatggcca cctgcccttc gacgccaagt
     1441 cgatcgatgc ctgcatcttg tcacacgcgc acatggatca ttcaggcgtg ctgccggtac
     1501 tcaccaagtt aggttacaac gggcacatct tctgcacctc cgccacccgc gatctatcca
     1561 cgctgatgct gcacgactcg gcgcacattc aagagagcga cgcgctgttc ttcaacaaac
     1621 acgcagccga gcgcagtctg ctgccgatca aaccgatcta cactgaagca gatgcccggg
     1681 agaccgttca acatttcgtc gtgtacgaat atcatcgccc gtttgaaccg atccccggcg
     1741 tccaagccac tttcttcgat gccggacata ttctgggttc aacttttatc acgctcgata
     1801 ttaccgaggg cgcgaagaaa ttccgattgg tcttcactgg cgatctcggc cgcaaacgcc
     1861 tgcccattct gcgcgatccc gaatcggccg atcgcgccga catctacatc accgaaggca
     1921 cttacggcgg gcggcatcac gccccgatcg aggatatgcg caatgaactc gcccagatcg
     1981 tcaaagacac tgccgatcgc aaaggcgtca tcgtcattcc ggctttcgcg gtcggccgca
     2041 cgcaagagat tgtatacgat ttgcagcagc tggtcgatca aaaacaaatt ccgaagctgc
     2101 cgatctacgt cgatagtccg ctggcgatca acgtcaccga ggtcttccgc ctgcatcccg
     2161 aatgttacga tgaagatatc aacagcttca tcaaatccga tccggatggt gacgctttcg
     2221 ggttttctca attgacctac gtgcgcgaat ccgcagcttc taaagcttta aacgatcgca
     2281 ccgatcccat gatcatcatt gcggcctccg gaatgtgcga agccggtcga gtgttgcacc
     2341 acctgcgcca tcaccttggc gatccgcgca gcacgatcat cttcgccggc tatcaagccg
     2401 agaacaccct cggtcgaaag attttagacg gtcagccgca cgtacgaatt ttcggcgaag
     2461 agcatgtagt gaaagcgcag atcaaaaaac tggaaggcta cagcgcccac ggcgatcatg
     2521 atgatctgat cggctttgcg cacaacatgg ctaagaaacc ctcacgcacc ttcatcgtgc
     2581 atgcagaata tgaagcggcg caggcattgc aaacaggctt gaccggcgtc ggcttgcaaa
     2641 atatcgctat acccaatcgc ggcgacttag tggaaatcta gatcgatgta gggctgtcag
     2701 ttcaagcgct ttttgcaaga aatctctaac gatcgagcga gagatttctc acttcgttcg
     2761 aaatgacaaa cgaaagtcct gcgatggttt gaagaaactt cgtttgtttg ttataatcat
     2821 tggcaaccgt tcggagaggt gccatagtgg ttgaattgga cggtctcgaa aaccgttgta
     2881 gcctttttag gttaccgtga gttcgaatct caccctctcc gccacgtagg gcaagatgtt
     2941 atcttgccct gcttttattt taggagattt tcaagcatgt cctcgctcac cgaatttcgc
     3001 agacaaaaag acgttttctt caaaaccgat cctcaatcgc cactaatgcc agatcaacgc
     3061 acgaactttg acagcttgag atattttcct gaaaacccgc aactgcgcat caccactaaa
     3121 gttgaggagt cttccgatca gcagatcgtg ccgatgatta ccagcacagg agcagtgaag
     3181 gaatattaca aatacggcca attctcgttt gaagtcgatg gacagacagc cacgctgcaa
     3241 gtctatcaag acgccgatcg cacttacttc ttcctgccgt ttgtcgatgc gaccgcgccc
     3301 gaagaaacct acggcgcggg acgctatctc gatctagaac cggagcatgg taacaccttc
     3361 gtgatcgact tcaattatgc atacaatccg tattgcgcct acaacgaaaa ttggaactgc
     3421 cccattcccc ccagagaaaa tcgcctcgcg gtgagaattg aagcgggtga gaagaccttc
     3481 aaaaaggatc tttgactttt gactgccccc agaatcagca gcccgctgtt gatcgtgaac
     3541 tagggcgaca agccgcaact cagaaatctt aacgatgctt ccaaatgggg gcgcgtttct
     3601 ccatgaacgc cgctaacccc tcgtgtgcat cttcagtcga catcaactcg tcaagataca
     3661 atttctccat tgtatccaaa gcacccacac gcatcgcgcg cttgcacagc cgcaaggccg
     3721 cgccactcaa gttgatcagc ttgccgatga gttgatcgac agttcgatcg agttgatcga
     3781 tcggcacagc gcgattgatc aagccgatct gcgcggcctc gatcgatccg atcggctcac
     3841 cggtgaacaa caactcggcc gcgcggcgag agccgatcag cacaggcaag cgcagcgcgg
     3901 cgatcggcgc gatggtcgcc aacttgattt ccggttgacc aaagttcgct ccttcagccg
     3961 cgacgatcaa atcgcagcac aatgcaagtt cgcaaccgcc gcccaacgcg tgcccgtgta
     4021 c
//
LOCUS       DASRTJ010000290         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_319971,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000290 DASRTJ010000000
VERSION     DASRTJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319971"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFF70_05910"
     CDS             complement(<1..784)
                     /locus_tag="VFF70_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HZY44269.1"
                     /translation="MPFSPVATSIACPFCHQPITIRVRQVVDVNQEPQLKGQLLAGQL
                     NAFTCPNCKNSGALASPFLYHDATKELALMFVPIELNLREDDRQRMIGQLTNSVLNSL
                     QPEQRKAYLLQPQQFFSLKTLVETILNADGVTPEMLKADQDKVDLIQKMMDTTDELAL
                     TALITEHAANIDLTSFQIVSSALSAASTDRQRVEYDRLLHVRDRMLELTPVGQKIKQQ
                     QAVLDSFTANPTRETLSAQLQLAADAETREALLTLGRPLLDYP"
     gene            complement(869..>1638)
                     /locus_tag="VFF70_05915"
     CDS             complement(869..>1638)
                     /locus_tag="VFF70_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44270.1"
                     /translation="ITYAALFSPDRQLVNIIANVRDMTHLREADDLKNTFISVISHEL
                     KTPVALIKGYAGTLRREDARWNQKTVRDSAGVIEEEADRLTQLIDNLLDASRLQAGAL
                     KLNVGDVALNQLAAHVVEKLQVQAPSHQLAIDFPADFPSVPADADRIEQVLNNLINNA
                     VKYSTAGTSIRVSGRVQPREVIITVADQGVGIPPEEQAHIFDQFYRVDNALTRRTQGS
                     GLGLYLTKAVVEAHHGRIWLESTPNNGSAFSFALPRE"
BASE COUNT          364 a          423 c          457 g          394 t
ORIGIN      
        1 atggataatc gagcagcggc cggccgagag tcaacaacgc ttcgcgcgtt tcagcatcgg
       61 ccgccaactg caactgtgcc gacagcgtct cgcgggtggg attggccgtg aagctatcca
      121 gcaccgcttg ctgctgcttg attttttgtc cgaccggagt gagttccaac attcgatcgc
      181 gcacgtgcag caaacgatcg tattcgacgc gctgtcgatc cgtagaggcc gcgcttagcg
      241 ctgaggacac aatttgaaag gaagtgaggt cgatgttggc tgcatgctcg gtgattagcg
      301 cggtcaaggc caactcatcg gtcgtatcca tcatcttttg aatcagatca actttgtctt
      361 gatcggcttt caacatttca ggcgtgacgc cgtcggcatt caagatcgtt tcgaccagcg
      421 ttttcaaact gaagaactgt tgaggttgaa gcaaataggc tttgcgctgt tcaggctgca
      481 agctgtttag aaccgaattg gttagctggc cgatcatgcg ctggcgatca tcttcgcgca
      541 gatttagttc gatcggcacg aacatcaacg cgagttcttt ggtcgcatcg tgatacaaaa
      601 acggtgaagc caatgcgccg ctgttcttgc aattagggca ggtaaacgca ttgagctgac
      661 cggccaagag ctgccctttc aactgcggct cttgattgac gtcgacaacc tgacggactc
      721 tgatagtgat cggttgatga caaaacgggc aagcgatgga cgtagcaacg ggggaaaagg
      781 gcatggttat ttctgtttcc taattttgga ttttgcaggg gccggcagct gattgcctgg
      841 acgagcagcc gtttacctct acaatgattt attctctggg taacgcaaac gaaaatgcag
      901 aaccgttatt aggtgtgctc tccagccaaa tgcggccgtg atgagcttcg acgacggctt
      961 tggtgagata caagcccagt ccactgccct gtgtgcggcg ggtcaacgca ttatcgacgc
     1021 gatagaattg atcgaaaatg tgcgcctgct cttccggcgg aatgcccaca ccttgatcag
     1081 ccaccgtgat gatcacttcg cgcggctgga cgcggcccga tacgcgaatg gatgtgccgg
     1141 ccgttgaata cttgacggca ttgttgatca agttattcag cacctgctca atccgatcgg
     1201 catccgccgg aacgctggga aagtccgctg ggaaatcgat ggcgagttga tgcgacggcg
     1261 cttgaacctg taatttttcg acgacatgcg cggccaactg attcaacgcc acatcgccga
     1321 cattcagttt caatgcaccg gcttgcagcc gcgaggcatc cagcagattg tcgatcagct
     1381 gcgtcaagcg atccgcttct tcttcaatga cgcccgcgct gtcccgaacg gttttctgat
     1441 tccagcgcgc atcttcacgc cgcagcgtac ccgcataacc cttgattaaa gccacgggcg
     1501 ttttcaattc gtgcgagatg acggagatga aagtattttt gagatcgtcg gcttcgcgca
     1561 gatgggtcat atcgcgcacg ttggcgatga tgttaaccaa ttgtcgatcg gggctgaaca
     1621 gcgcagcata ggtgatgc
//
LOCUS       DASRTJ010000291         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_320024,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000291 DASRTJ010000000
VERSION     DASRTJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320024"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="fni"
                     /locus_tag="VFF70_05920"
     CDS             complement(<1..706)
                     /gene="fni"
                     /locus_tag="VFF70_05920"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019507905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZY44271.1"
                     /translation="MTIEHRKADHIRINLDEDVGFDRLTVGFERYRFIHQALPELDLK
                     LIDLSTRLFGKILKAPVLISSMTGGTDQAQQINLNLAQAAQQYGVAMGIGSQRASLVR
                     PETAHTFKVRSVAPDILLFANLGAVQFNYGYGVDECKRVIDMIEADALVLHLNPLQEA
                     VQPEGDVNFAGLVKKIEAICKSIGVPVIGKEVGWGISEQAAKMLADAGVAAIDVAGSG
                     GTSWSQVELFRAPDNLH"
     gene            928..1185
                     /gene="acpP"
                     /locus_tag="VFF70_05925"
     CDS             928..1185
                     /gene="acpP"
                     /locus_tag="VFF70_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZY44272.1"
                     /translation="MADGTTYEKIQGIISELLGVDKSKVTMEARFREELEADSLDLVE
                     LIMAFEEEFGQEISDEDAQKITTVGEAVRYIDERKKVEPAA"
     gene            1308..1793
                     /locus_tag="VFF70_05930"
     CDS             1308..1793
                     /locus_tag="VFF70_05930"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZY44273.1"
                     /translation="MENKHSAESKVVLSQFMQPEHANVMGNVHGGVLMKLIDEAGGLC
                     ATRHARRPTVTVAVDSLRFLSPVHVGDLVTFTAHLNYVGRTSMEADVHVEAENILTGE
                     RTHTNDAYLVYVALNDLGKPIEVPRLIVETPEEQRQWELGKQRQADRLARAQREGSHA
                     T"
     gene            1783..2241
                     /locus_tag="VFF70_05935"
     CDS             1783..2241
                     /locus_tag="VFF70_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY44274.1"
                     /translation="MPLKEEYRAAGGVLTRDDGKLFLVLERPGRPEVRLPKGHIEAGE
                     TTAQAAVREVQEETGYADIAIAADLGSITHSFYVTARDMEVTRTESYFLMRLIGEKRY
                     DGPQFAHENFRRRWIDCDEAERLLTYEVEREFARRAKAALMANTSGETRA"
     gene            2234..>2815
                     /locus_tag="VFF70_05940"
     CDS             2234..>2815
                     /locus_tag="VFF70_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44275.1"
                     /translation="MRNFSGRSMVLLIALALIAVGYFGPWVAHKDAGLILSADDLAEF
                     VKFMPIIRSGEAGIVRELFFAPIWFSSIGFALFGGRRRSVVAKMGLLLLSLLLVFTPL
                     PPFTFLIEAYRSPEFGLTFWATVIAILSILVLTIFGHRLSDRAEAIAWIVLGLAAASI
                     APLHFIKIQPEIAKLYPFSLGWGCVMVIVGGVAL"
BASE COUNT          644 a          657 c          822 g          692 t
ORIGIN      
        1 tatgcagatt gtcgggtgca cggaataatt cgacttgact ccaggatgtg ccgcccgatc
       61 cggccacgtc gatcgccgcg acaccggcat cggctagcat ttttgcggct tgttcgctga
      121 tgccccaacc gacttctttg ccaatgaccg gcacgccaat tgatttgcag atcgcctcga
      181 tcttcttgac gagtccggcg aaattcacat cgccttcagg ctgcaccgct tcttgcagcg
      241 gattgagatg caagaccaat gcatcggctt cgatcatatc gatgacgcgt ttgcattcat
      301 cgacgccgta accgtaattg aactgcactg cgccgagatt cgcaaacaac aaaatatctg
      361 gcgcgaccga tcgaactttg aaagtgtgcg ctgtttccgg tcgaacgaga ctggctcgct
      421 gcgaaccgat acccatcgct acaccgtatt gttgtgcggc ttgcgcgagg ttgagattga
      481 tctgttgggc ttgatcggtg ccgccggtca tcgaagagat caacactggg gctttgagga
      541 ttttgccgaa caatcgggtg ctgagatcga tcaatttgag atcgagttcg ggcaaagcct
      601 gatggatgaa gcggtagcgc tcgaagccga ccgttaatcg atcgaatccg acatcttcat
      661 cgagattgat gcggatatga tcggctttgc gatgctcgat ggtcatgtat tctctcgata
      721 aacgtaaggc tagattaccg cccgtataaa acagaccgga cactgtcttg ccctagtgcg
      781 taacttttct aaaaatgaag tgttgtagtt gcggtgaggg tatggtaaca cgtccatcat
      841 ggatgtcaag gacatcttga caatttccag tcggtggtta caatatgtcc attcattcac
      901 acgcgtacaa cccaaggagc tgagcacatg gcagacggca caacctacga aaagattcag
      961 ggcatcataa gtgaattgtt gggggtcgac aaatccaagg tgacgatgga agcgcgcttc
     1021 cgcgaagaac tggaagcaga ttcgcttgac ttagtcgagt tgatcatggc ctttgaagaa
     1081 gaatttggcc aggaaatttc cgacgaagac gcgcaaaaga ttaccaccgt cggcgaagcg
     1141 gttcgctaca tcgatgagcg caagaaagta gaaccggcgg cttagccgat tgattttctt
     1201 gacaagggga tatgatcgcg gccaccggtt tcgtatcggt ggccgctttt tgtattgtcg
     1261 gaaaaaagtg aaaattaagc ggagtgtcac taaacgcgga ggggcgagtg gaaaataaac
     1321 attcagctga atcaaaagtc gtgctttcgc aattcatgca gccggaacat gctaacgtta
     1381 tgggtaacgt acatggcggt gtgttgatga aattgatcga tgaagcgggt ggtctgtgcg
     1441 ccacacggca tgcgcgacgg ccgacggtta ccgtggcggt tgactcgctc cgatttttgt
     1501 cgccagtcca tgtaggcgat ctggtaacct tcactgcaca ccttaattac gtcggacgca
     1561 catcgatgga agccgatgtg catgtggaag cggaaaatat tttgacgggc gagcgcacac
     1621 acaccaacga tgcttatttg gtgtatgtgg cgctaaacga tctcggcaaa ccaatcgagg
     1681 tgccgaggct gatagtggag acgcctgaag aacaacggca gtgggaattg ggcaagcaac
     1741 ggcaagccga tcgattggcg cgagcgcaac gcgagggtag ccatgccact taaggaagaa
     1801 tatcgagcag cgggcggcgt gctgacgcgc gacgacggga aactattttt agtgttggag
     1861 cgtccgggtc gacctgaagt gcgcttgccg aaaggccata tcgaagcagg cgaaacgacg
     1921 gcccaagccg cagtccgcga agtccaagaa gagacgggtt acgcagatat agcgattgcc
     1981 gccgatctgg gctcgattac gcattcgttt tacgtcacgg cgcgtgatat ggaagtcacg
     2041 cgcacggaat catatttttt aatgcggttg attggtgaga agcggtacga cgggccgcaa
     2101 ttcgcgcacg aaaattttcg gcggcggtgg atcgattgcg atgaggccga gcgtttgttg
     2161 acttacgaag tggagcgtga attcgcacgg cgtgccaagg ctgcgttgat ggctaacacc
     2221 agcggagaaa ctcgtgcgta atttttccgg gcgatcgatg gtgctgctga tcgcgttggc
     2281 gttaatcgct gtcggctatt ttgggccgtg ggttgcgcat aaagatgccg gtttgattct
     2341 atccgccgac gatctggccg agttcgtcaa gtttatgccg atcattcgat cgggcgaagc
     2401 gggcattgtg cgcgagttgt tttttgcgcc gatctggttt agctcgatcg gtttcgcgtt
     2461 gtttggcggg cgtcggagat ccgttgtggc gaagatgggt ttgctccttt tgagtttact
     2521 gcttgttttc acgccgctgc cgcctttcac cttcttgatc gaagcgtatc gatcgccaga
     2581 gttcggttta accttttggg ctactgtgat cgctatattg agcatactgg ttttaacgat
     2641 ctttgggcat cgattatccg atcgagctga agcaattgca tggatcgttt tgggacttgc
     2701 cgcggcttcg atcgcaccgc tgcacttcat caaaattcaa ccggagatcg ccaaattata
     2761 tccgtttagt ctcggttggg gatgtgtgat ggtgatcgtc ggcggtgtgg cgcta
//
LOCUS       DASRTJ010000292         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_272422,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000292 DASRTJ010000000
VERSION     DASRTJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272422"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFF70_05945"
     CDS             complement(<1..567)
                     /locus_tag="VFF70_05945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZY44276.1"
                     /translation="MSKTNGKSDRDVVIVSAARTPIGKFMGALSSLSSTQLGAVAVRE
                     AVTRAGIDAKLIDEVIMGQVVAAGAGQSPGRQAAIFAGLPDTVGAISVNKACGSGLEA
                     VMLAAAMIKAGDGDIYVSGGMESMTNAPYLLPSGRTGNRYGDVKLLDSVIHDGLWCAF
                     ENHIMGNSAEFIADKYEVTREEMDDFSLK"
     gene            complement(638..1147)
                     /locus_tag="VFF70_05950"
     CDS             complement(638..1147)
                     /locus_tag="VFF70_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZY44277.1"
                     /translation="MEHLWRDRMAYISGATRPPEGLCVFCEAPKHADDTVFILQRGQF
                     AYVIMNIFPYNNGHVMVNPYAHVDSIEKLDDATLTEIMQLTNLSLAAIRSAAQPHAFN
                     LGANIGQAAGGGIKDHFHFHIVPRWQGDTNFMPIVAETRVLPEKLEDSYQRLKLAFDQ
                     IKLQMTNDK"
     gene            1221..>1946
                     /locus_tag="VFF70_05955"
     CDS             1221..>1946
                     /locus_tag="VFF70_05955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZY44278.1"
                     /translation="MPLTPESIRAALSDQPLIKQVLHTHSIGSTNDLARQLANDGAEE
                     IALISTNEQTAGRGRQGRSWFTPRGSALAISLLTRPAIPAPRAMLLTMLAGLAVVEGI
                     EDATNLHLDLKWPNDVVSIANDQMPNAKLRKVGGILTECSFQNDQIEYAIVGIGLNVD
                     VDFSQQESLRNSATSLIELSGLPIDQLVVLKAIVTRFIDHYSWLGNEVKLRDAWRARL
                     INLGQHVSVQSGAEILEGLAEAVD"
BASE COUNT          461 a          542 c          485 g          458 t
ORIGIN      
        1 cttcaatgaa aagtcgtcca tctcttcacg cgtgacttca tatttatcgg cgatgaactc
       61 cgccgagttg cccatgatgt gattttcgaa cgcgcaccac agcccgtcat gaatcacact
      121 atccagcagc ttcacatcgc catagcgatt accggtgcgt ccactcggca gcaggtacgg
      181 tgcgttggtc atgctctcca tgccgcccga aacataaatg tcgccgtcgc ccgctttgat
      241 catcgcggcg gccaacatca ccgcttcaag accggagccg caagctttgt tgacgctgat
      301 cgcgccgacc gtgtccggca gtccggcaaa gatcgcggcc tgccggcccg gcgattggcc
      361 cgcgcctgcg gcaacgactt gccccatgat cacttcatcg atcaatttgg catcgatgcc
      421 ggcgcgggtc actgcttcac gcaccgctac agcgcccaat tgggtggatg ataatgaaga
      481 gagtgctccc ataaatttac cgatcggcgt acgcgccgca ctgacaatca ccacatcgcg
      541 atcggatttg ccgtttgttt tactcatcga tcctcctact ggagtcattt gctacttatt
      601 gaacaacgct ttggcgaata tgactaatgc caattgttca tttgtcatta gtcatttgta
      661 attttatttg gtcaaatgcc agctttaatc gctgatagga atcttctaat ttctccggca
      721 gcacccgcgt ctcggcgacg atcggcatga agttggtatc gccctgccag cgcggcacaa
      781 tatgaaaatg aaaatgatct ttaattccac cgccggcagc ttgaccgata ttcgcaccga
      841 ggttaaacgc gtggggctgg gcagccgatc ggatggccgc cagacttaaa ttcgtcaact
      901 gcatgatctc ggtcaacgtc gcatcatcca gtttctcgat cgaatcgacg tgcgcatacg
      961 gattaaccat cacgtgtccg ttgttatacg gaaagatatt cataatcaca tacgcaaact
     1021 ggccgcgctg caaaatgaaa actgtgtcat ccgcatgttt gggggcttca caaaaaacac
     1081 agaggccttc aggcggcctg gtcgcaccac tgatgtaagc cattcgatcg cgccacagat
     1141 gttccatcaa tcactccgta cctattttac ctcagatcga atgagtagcg catcgcctcg
     1201 ctccacgcta caattgcagc atgccactga cgcccgaatc gatccgcgcc gcgttgtccg
     1261 atcaaccgct gatcaaacaa gtactgcaca ctcactcgat cggctcgacc aacgatctcg
     1321 cgcggcagct ggcgaatgac ggcgctgaag agatcgcgtt gatctcaacc aacgagcaaa
     1381 ctgccgggcg cgggcgtcag ggtcgatcgt ggttcacgcc gcgcggatca gctttagcga
     1441 tctctttatt aaccagaccg gcgatcccgg ctccacgcgc gatgctgctg acgatgctgg
     1501 ccggtttagc ggtggttgaa gggattgaag atgcaaccaa cttacatttg gacttaaaat
     1561 ggccgaatga tgttgtctca atagcaaatg atcaaatgcc aaatgccaaa ttaaggaaag
     1621 tcggcggcat tttgactgaa tgctcttttc aaaacgatca aatcgaatat gctatcgtcg
     1681 gcatcggcct gaatgtggat gtcgattttt cgcagcagga aagtctgcgc aacagcgcaa
     1741 cgagtctaat cgaactcagc ggactgccga tcgatcaatt ggtcgtgttg aaagcgatcg
     1801 tgacgcgctt cattgatcac tatagttggc ttggcaatga agtcaagctg cgcgatgcct
     1861 ggcgggcgcg cttgatcaat ttgggccaac acgtcagcgt ccaatcaggc gcagaaattc
     1921 ttgaaggttt agctgaagcg gtggac
//
LOCUS       DASRTJ010000293         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_92574,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000293 DASRTJ010000000
VERSION     DASRTJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92574"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFF70_05960"
     CDS             complement(<1..431)
                     /locus_tag="VFF70_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZY44279.1"
                     /translation="MQEAVIKFGTDGWRGQIADDYTFANVRRCAQGFAAYLQAKKLAD
                     RGVVVGYDKRFDSEYFAAACAEVLAANGIKVWLTDRATPTPVISYAVVHHTAGGAINI
                     TASHNPPTDNGFKVRDPNGGAIDPAGLTEIESRIPSSDAVKS"
     gene            complement(435..1409)
                     /gene="gmd"
                     /locus_tag="VFF70_05965"
     CDS             complement(435..1409)
                     /gene="gmd"
                     /locus_tag="VFF70_05965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZY44280.1"
                     /translation="MNNKRALITGITGQDGSYLAEFLLDKGYEVIGMVRRTSTINFDR
                     IKHFQDQVEIVQGDLLDQSSLIEILRENKPQDVYNLAAQSFVPTSWKQPTLTGEFTAL
                     GVTRMLEAVRTVDPSIRFYQASSSEMFGKVQEIPQTEKTPFWPRSPYGVAKVYGHWIT
                     VNYRESYNLFACSGILFNHESPRRGLEFVTHKISHAVAKIKLGQAKDLHLGNIESKRD
                     WGFAGDYVRAMWLMLQEDQPDDYVIATGETHSVREFCELAFDRVGLKWDKYVVQDSAF
                     RRPAEVDHLIGDASKAGSKLGWEPTVSFPELVNMMVDADLEALKKNGA"
     gene            complement(1471..2418)
                     /locus_tag="VFF70_05970"
     CDS             complement(1471..2418)
                     /locus_tag="VFF70_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZY44281.1"
                     /translation="MRALITGAAGFVGSHLADVIHTETDWQIWGTILGVEDPRSVPVD
                     GSILKVDLRDPDQVRAAIEQAQPDFIFHLAGQAYVPQSWNDPWATYETNIRSQLNLLQ
                     AVSAIGLRPKIMVVGSNEEYGWVSESDLPIDEDTPLRPNSPYSVSKIAQDFMGLQYFL
                     DRDLPVVRVRPFNHIGPRQNERFVAPSFAKQIIEIERGLKPPVLKLGYMSARRDFTDV
                     RDMCRAYILAVQKGEPGEMFNIGSGQARSVRELLDMMLAHSSIKVTEEADPSKMRLSD
                     VPVSYCDPTKFKQRTGWEPRITFEQTVIDILDDWRERIK"
     gene            complement(2617..>3698)
                     /gene="ahcY"
                     /locus_tag="VFF70_05975"
     CDS             complement(2617..>3698)
                     /gene="ahcY"
                     /locus_tag="VFF70_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZY44282.1"
                     /translation="HTLQLGGADIVLCASNPLSTQDDVAASLVANYEIPVYAIKGEDN
                     TTYYQHIKAALDHQPHMTMDDGADLVSLLHKERTELLKDVYGGTEETTTGVIRLRAMA
                     KEGALKFPIVAINDANTKHMFDNRYGTGQSTLDGVIRATNVLLAGKTIVVGGYGWCSR
                     GIAQRAHGMGGHVIVTEIDPLKALEAVMDGFEVMTMAEAASKGDIFISATGDIHVIDT
                     PHFEKMKDGALVANSGHFNVEINIPALEKLSNGAPRKVREFVDQYTLKNGRKINLLGE
                     GRLINLAAAEGHPASVMDMSFANQALSAEYMAKNAKSLKPAVYSVPEDIDKEVARLKL
                     AAMGVKIDTLTPEQQKYLESWQEGT"
BASE COUNT          863 a         1064 c          900 g          871 t
ORIGIN      
        1 gacttcactg catcgcttga aggaatgcgt gactcgatct cggtgaggcc ggccggatcg
       61 atcgcgccac cgttcggatc acgcactttg aagccgttat ccgtcggtgg attatgcgag
      121 gccgtgatgt tgatcgcccc gcccgctgtg tgatgcacaa ccgcatatga aatcacgggt
      181 gtcggcgtag cccgatccgt tagccagact ttgatgccat ttgcggccaa cacttccgca
      241 cacgccgctg caaaatattc cgaatcaaag cgcttgtcgt atccaacgac tacgccacga
      301 tccgccaact tcttcgcttg aagatacgcg gcgaaaccct gagcgcagcg gcgcacattc
      361 gcgaaggtgt aatcatccgc aatttgtccg cgccagccat ccgtgccaaa tttgataaca
      421 gcctcttgca tgggttacgc tccgttcttt ttcaacgctt ccagatcagc atcgaccatc
      481 atgttgacca gttccggaaa cgatacggtt ggttcccaac ccaatttcga tccggctttg
      541 ctcgcatcgc cgatcaagtg atcgacctcg gcgggccgcc tgaaggccga atcttgcacg
      601 acatacttat cccacttcaa gccaacgcga tcgaaagcca attcacagaa ctcgcgcacg
      661 gaatgcgtct cgcccgtggc aatcacgtaa tcgtccggct gatcttcttg caacatcaac
      721 cacatggcgc ggacgtagtc gcccgcaaaa ccccaatcac gtttcgattc aatgttgccc
      781 aagtgcaaat ctttggcttg gcccaatttg attttggcaa ccgcatggct aattttgtgg
      841 gtgacaaatt caaggccgcg ccgtggcgat tcgtgattaa acaaaattcc agagcaggca
      901 aacaagttat agctttcccg atagttcacc gtgatccagt ggccgtacac tttggcgacg
      961 ccatacggac tgcgcggcca gaacggcgtc ttttcggtct gtggaatttc ttgcaccttg
     1021 ccgaacattt cactgctgga ggcttgataa aaacggatcg acggatcgac cgtgcggaca
     1081 gcttccagca tgcgcgtcac acccagcgcg gtgaattcgc cggtcaacgt cggctgcttc
     1141 cacgaagtcg gcacaaacga ttgggcggcg agattataaa cgtcctgcgg tttattctcg
     1201 cgcagaatct cgatcagcga actttgatcg agtagatcgc cctgcacaat ctccacttga
     1261 tcctggaagt gcttgatgcg atcgaagttg atcgtgctgg tgcggcgcac cataccgatg
     1321 acttcgtagc ctttgtccag caaaaattca gctaaatagg atccatcctg gccggtgata
     1381 cctgttataa gtgcgcgttt gttgttcaag agatcatcct tgctctactc gtaactcatt
     1441 aactaacgag tgatgagtaa cgagtaaaaa tcatttgatt cgttcacgcc aatcatccaa
     1501 tatatcgatc acggtttgct caaacgtgat gcgcggctcc caaccggtgc gctgcttaaa
     1561 cttcgtcgga tcgcaatacg agaccggcac atcactaagc cgcattttcg atggatcagc
     1621 ctcttccgtc accttaatcg aactgtgcgc caacatcata tcgagcagct cgcgtaccga
     1681 ccgggcttgc cctgatccga tgttaaacat ttcacccggc tcgccttttt gcacggccaa
     1741 aatatatgcg cggcacatat cacgcacatc agtaaaatcg cgacgggccg acatgtaacc
     1801 caacttcaac accgggggct tcagaccgcg ttcgatctca atgatctgct tggcaaagga
     1861 tggcgcgaca aaacgttcat tctgccgcgg accgatatga ttgaacgggc gcacccgcac
     1921 caccggcaaa tcccgatcga gaaaatattg caaacccata aaatcctgtg cgatcttgct
     1981 gacggaataa gggctattgg gacgcaacgg agtatcttcg tcgatcggga gatcggactc
     2041 actaacccag ccatactctt cattcgatcc caccaccatg atcttgggcc tgaggccgat
     2101 cgctgaaacg gcttgcaata agtttaactg agatcgaata ttggtttcgt aagtagccca
     2161 cggatcgttc cacgattgcg gcacataggc ttgacccgcg agatggaaaa taaaatcggg
     2221 ttgcgcttgc tcgatcgccg cgcggacttg atcgggatcg cgcagatcga ctttaagaat
     2281 cgaaccgtcc acgggcaccg atcggggatc ctcgactccc agaatcgtgc cccaaatttg
     2341 ccaatcggtc tcggtatgaa tgacatcggc caaatgactg ccgacaaaac ccgccgcgcc
     2401 tgtaatcaat gcccgcaaaa tttgtatcct gttcgaaatt tcgcaagtat tataccggat
     2461 ttactttgat gcggcaaaga gcggacaaat aaaccacgga ttacacagat gacactggtt
     2521 tttcttgccc aacacgtaca aagtccgact gtcaatcagg cgatcaacaa tcggacagtg
     2581 caaatcacag atcagaaatc aatgatcaga atttctttag gtgccttcct gccacgattc
     2641 aagatatttc tgctgctctg gcgttagcgt atcgatcttc acacccatcg cggccagctt
     2701 caagcgcgcg acttctttat cgatatcttc cggcaccgaa taaacagccg gcttcagcga
     2761 cttggcattc ttggccatgt attccgctga taacgcctga ttggcgaatg acatgtccat
     2821 caccgaagcc gggtggcctt cggcggcggc gagattgatc aagcggcctt cacctagcaa
     2881 gttgatcttg cggccgttct tgagcgtgta ttgatcgacg aactcgcgca ctttgcgggg
     2941 cgcgccattc gacaatttct ccagcgcggg aatattgatc tcgacgttaa aatgaccgga
     3001 gttggcgacg agtgcgccgt ctttcatctt ctcaaaatgt ggcgtatcga tcacatgaat
     3061 gtcaccagtg gcactgatga agatgtcgcc tttcgacgca gcctcggcca tcgtcatcac
     3121 ttcgaagcca tccatcaccg cttccagcgc cttgagtgga tcgatttcgg tgacaatcac
     3181 atgcccgccc atgccatgcg cgcgctgtgc aatgccgcgt gagcaccaac cgtagccacc
     3241 caccacaatc gtcttgccgg ccagcaaaac attcgtcgcc cggatcactc catcgagcgt
     3301 ggattggccg gtgccataac gattgtcgaa catgtgcttg gtgtttgcat cattgattgc
     3361 gacaatcggg aatttcagcg cgccttcttt tgccatggcg cgtaagcgaa tgacgcccgt
     3421 cgtcgtctct tcagtgccgc catacacatc cttcaacaat tccgtccgct ctttgtgcaa
     3481 cagcgagacc aaatcggcgc cgtcatccat ggtcatgtgc ggctgatggt cgagcgcggc
     3541 tttgatgtgc tgatagtacg tggtgttgtc ttcgcctttg atggcataca ccggaatctc
     3601 ataattcgcg accagcgagg cggccacatc gtcctgcgtg ctcaacggat tcgacgcgca
     3661 cagcacaata tctgcgccgc ctaactgcag ggtgtgca
//
LOCUS       DASRTJ010000294         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_196624,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000294 DASRTJ010000000
VERSION     DASRTJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196624"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFF70_05980"
     CDS             <1..603
                     /locus_tag="VFF70_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44283.1"
                     /translation="RFVSAIISLVVFSLACSLGSSTNGSNTNQQPGVQQPAGTPLFAT
                     QSSTLPPMASTFPPIASPPPATQSATTGANGATAQPTGSTAAPQAATTSAPKPSTATK
                     TKVPATSSPLTVKYEVVKVSRLPDNQAALTIHVIASGGAGGYKYYNDNVLKAGDTFDV
                     PGQCGHPFNHTIKVQAADGQFVSLSYFVAGQCPTPTATPP"
     gene            690..1292
                     /locus_tag="VFF70_05985"
     CDS             690..1292
                     /locus_tag="VFF70_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44284.1"
                     /translation="MRRRILLGWLTLFMLSMAACSPDSSGSAANPTSAPALTAAPQSL
                     PIFAPTNAPIGAPTTNATQAPLIGVATATPVTGTDLITVTPTSEATSAATSEATSAPL
                     STQPPPPPPPSSGPLDFTVDVAGCKVDPSRAGGVIMTMRFNATGGSGLYTYYDEGQVV
                     SQIFDRPATKGSSVIDAFRVDSGDQSISHKIQFKPNDYCH"
     gene            complement(1385..>2689)
                     /locus_tag="VFF70_05990"
     CDS             complement(1385..>2689)
                     /locus_tag="VFF70_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZY44285.1"
                     /translation="SGDPTLVRNDIDVNFDWGTALPAPGLPADNFSVRWTRSLSFQAQ
                     IYRVTVQADDGVRVWIDNNLLIDEWHLGGGVAFMHDVNVAAGSHAIKIEYYEGSGLAA
                     IHFALVPVETYGDWKGEYFDNPFLGGTPVLVRNDTAVSFNWGITSPGQGLPPDKFSVR
                     WTRGATFPAGIYRFTLQADDGVRFYIDGYLMIDEWHDSSTKSYSHEVNLGGGLHNLTI
                     EYYENTGNANVGITYQLANDITHWNGTYFTNDQWTGLPTFVRDDDALNFDWAAGSPDP
                     LIPVDHFSVRWTRTLALSAGTYRFEMTVDDGVRFYVDDVLVVDQLHVSNSVPYFVQLT
                     LAQGNHKFRIDYAEYTGLARFSWSMINLSATPTFTSTPTKTPTPTSTGTSTPTGTPTG
                     TSTSTATPTETATATATPTITPTSSPSLTATPTITSTVVITP"
BASE COUNT          634 a          797 c          692 g          566 t
ORIGIN      
        1 cgatttgtca gcgcgatcat cagcttagtg gtcttcagct tggcctgctc gttaggcagc
       61 agcacaaatg gatcgaacac caatcagcag cccggcgtgc aacaaccggc gggcacgccg
      121 ctgttcgcga cccaatcttc gacgctgccg cccatggctt caaccttccc gcccatcgca
      181 tcgccgccac cggcaacaca atcggccacc actggcgcaa atggtgcaac agctcaacca
      241 accggatcga cggcggcacc tcaagcagca acgacctcag cgccgaagcc gtccaccgcc
      301 accaaaacca aagtgcccgc aacgtcctcg ccgctcaccg taaaatatga agtcgtgaaa
      361 gtctcgcggc tgcctgacaa tcaagctgca ttaacgattc atgtcattgc ctctggcggc
      421 gcaggcggct acaaatacta caacgataat gtcctcaaag ccggcgacac atttgacgtg
      481 cccggacaat gcggccatcc atttaatcac accatcaaag tccaagccgc ggatggtcaa
      541 ttcgtgtcgt tgtcttattt tgtggctggt caatgcccga caccaaccgc aacaccgccc
      601 tagtcaattt cgatttcgat ctttgattta ttcggctaac ttcaacatgt gatctatcac
      661 gcctcattct gacttaagga gatccttcaa tgcgacgtcg tattctgctt ggctggttaa
      721 cgctattcat gctgtcgatg gcagcttgtt cgccggactc cagtgggtca gcagctaacc
      781 cgactagtgc tccggccctg acggctgcac cgcaatcgct gccaatattt gcaccgacca
      841 acgcaccgat cggcgcgccg actacgaatg ccacccaagc gccgctcatc ggcgtcgcca
      901 ctgccacacc ggttacagga actgacctga ttaccgtgac gccgaccagc gaagccacga
      961 gtgcggcaac atcggaagcg accagcgcac cactatcgac tcaaccgccc cctccgccac
     1021 cgccatcgag tgggccgctt gacttcaccg tcgatgtggc cggttgtaaa gttgatccga
     1081 gccgcgcggg cggcgtgatc atgacgatgc gttttaatgc aacaggcggc agtggcctgt
     1141 acacctatta cgatgaaggc caagtagtat cacaaatctt cgatcgtccc gccaccaaag
     1201 gctcaagtgt catcgacgcg ttccgggttg attcgggcga tcaatcgatc agccataaaa
     1261 tccaattcaa gccgaacgat tactgccact aagaaaagtt acacatcaaa aaagctgtgc
     1321 tcagctaaag ctgggcgcag cgttttatat gcgcaaccga attatcctcg cttaacgaat
     1381 cgatctacgg cgtaatcacc acagtggagg tgatcgttgg cgttgcggtc agcgaaggcg
     1441 acgatgttgg ggtaatggtc ggcgtcgccg tggccgtagc cgtttctgtc ggcgtggccg
     1501 ttgaggtcga tgtgcctgtc ggtgttccgg tcggcgttga ggtgccggtc gacgtcggtg
     1561 tcggcgtctt cgtcggagtt gaagtgaacg tcggcgttgc cgatagattt atcatcgacc
     1621 aggaaaaacg cgccaggcca gtgtattcgg catagtcgat tctaaatttg tgattgcctt
     1681 gggctaaggt taattgcaca aagtacggca ccgagttaga tacatgcagt tgatccacta
     1741 ccaacacatc gtcgacgtag aagcgcacgc cgtcatctac cgtcatctcg aagcgatacg
     1801 tgcccgccga taaggccagc gttcgagtcc agcggacgga aaaatgatcg acgggtatca
     1861 atggatccgg cgagccggca gcccaatcaa aattcaacgc gtcgtcgtca cgcacaaatg
     1921 tcggcaggcc cgtccattga tcgttcgtga agtaggtgcc attccagtga gttatatcat
     1981 tcgccaactg ataggtaata ccgacgttcg cattgccggt attctcgtag tattcgatcg
     2041 tgagattgtg cagaccgccg cctaaattga cctcgtgcga atagctcttg gtggatgagt
     2101 catgccactc atcgatcatc aagtagccat cgatatagaa tcgaacgcca tcatcggctt
     2161 gcagcgtaaa gcgataaatg ccagcgggga aagtggcgcc gcgtgtccaa cgcactgaga
     2221 acttgtctgg cggcaagcct tgaccgggcg aggtgatacc ccaattgaaa ctcacggccg
     2281 tatcgttgcg aactaacacg ggcgtgccac ccaagaacgg attgtcgaag tactcgcctt
     2341 tccaatcgcc ataagtctcg accggcacaa gcgcaaagtg gatggccgcc aggccgctgc
     2401 cttcgtaata ttcgattttg atcgcatgtg agccggccgc cacgttgaca tcgtgcataa
     2461 aagcgacccc gccacctaaa tgccactcat cgatcagcaa gttgttatcg atccacactc
     2521 gcacgccatc atcagcctga actgttaccc gataaatctg cgcttgaaac gataagctgc
     2581 gcgtccagcg cactgagaaa ttatcggcgg gcaaaccggg cgcgggcagc gccgtgcccc
     2641 aatcgaaatt cacatcgata tcgttgcgga ctaaggtcgg atcgccgct
//
LOCUS       DASRTJ010000295         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_320324,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000295 DASRTJ010000000
VERSION     DASRTJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320324"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFF70_05995"
     CDS             complement(<1..637)
                     /locus_tag="VFF70_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZY44286.1"
                     /translation="MNHLCSTERSRLLNRTIIGLLLIAALVVACGQPTISKVSTAKDT
                     PRAAPSSSTQAPANSSTLNCTPPATLTPAQTEGPYFKAGSPERASLIEAKTTGIKLIL
                     TGYVVTADCKPIAQALMEFWQADTNGNYDNNGYTLRGHQYTDANGRYQLTTIVPGLYP
                     GRTEHIHVKVQAPNGPLLTTQLYFPGVTQNDSDGIFDPKMVIDVQQASDGLI"
     gene            complement(771..1202)
                     /locus_tag="VFF70_06000"
     CDS             complement(771..1202)
                     /locus_tag="VFF70_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HZY44287.1"
                     /translation="MKKRQRIIKVAAIILGVVVIANVVPWLLETNPSVVQEPAWDSPQ
                     TRALAQRACFDCHSNETVWPVYARIAPISWLVTWDTLNGRRHLNFSEWGVRRGEGDNE
                     TARVIQRGDMPPAYYIAQHPEAGLTANEKQQLIQGLQATLK"
     gene            complement(1199..>1765)
                     /locus_tag="VFF70_06005"
     CDS             complement(1199..>1765)
                     /locus_tag="VFF70_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZY44288.1"
                     /translation="ESVAEAERWPVQLTSRLRAEQIDLDEPIIIAKTGWATDELNAAI
                     DQLEPSSQYDLVSLLIGVNNQYRGYSADGYRSEFATLLKRAIGFAADRPARVIVLSIP
                     DWGVTPFAAGRARAKIAAEIDRFNAINRAETIRSGAQYIDITPISRRASIDPALIAND
                     GLHPSGKMYTHWTELAMPIAQQILKEGL"
BASE COUNT          375 a          482 c          472 g          436 t
ORIGIN      
        1 ctattaggcc atcgctagcc tgttggacat ctatcaccat tttgggatcg aaaatgccat
       61 cactgtcatt ttgcgttacg cctggaaaat aaagttgggt ggtcagcagc ggcccgttcg
      121 gcgcttgcac tttaacgtgg atgtgttccg tgcggccggg atacaagccc ggcacgatcg
      181 tcgtgagttg atagcgaccg ttcgcatcag tgtattgatg cccgcgcaga gtataaccat
      241 tgttgtcgta gttgccattt gtatcggctt gccaaaattc catcaatgcc tgcgcgatcg
      301 gtttacagtc agcggtgacg acatagcctg tcaaaataag tttgatgccg gttgtctttg
      361 cctcgatcag cgatgcgcgc tcgggcgatc ctgctttgaa gtacgggcct tcggtctggg
      421 caggcgtcaa ggtggccggt ggagtgcagt ttagcgttga tgaattggca ggtgcttgag
      481 tcgacgagga tggtgcggct cgcggcgtat cttttgcggt agaaactttc gatattgtcg
      541 gttggccgca agcgactaca agtgctgcga tcagcagaag gccaatgatt gttcggttta
      601 acaagcgcga tcgctcggtt gagcataaat gattcatgtc aatctcctcc gctggagata
      661 ataagtatcc aagagcattg ccaaacttgt caattgtgag tttcgtcgag catataaaag
      721 ccgcaaagca tttctctgca gctgtcagtt atcacttcgc aacgacggca ttatttcaaa
      781 gttgcttgca aaccttgaat cagctgctgc ttttcattcg cggtcagacc ggcctcggga
      841 tgctgggcga tataatacgc tggcggcata tcaccgcgtt ggatcacgcg cgcggtttca
      901 ttgtcaccct cgccgcggcg cacaccccat tcggaaaaat tcaaatggcg ccggccattt
      961 aaagtgtccc aggttaccag ccaggaaatc ggcgcgatcc gcgcatagac aggccagacg
     1021 gtctcattgc tgtgacagtc gaaacacgcg cgctgcgcaa gtgcgcgtgt ctgcgggctg
     1081 tcccacgccg gctcctgcac gacagacgga tttgtttcca ataaccacgg caccacatta
     1141 gcgatgacta ccacgcccag tatgattgcc gctactttga taatgcgttg gcgcttcttc
     1201 ataatccctc tttcaatatt tgctgtgcga tcggcattgc caactctgtc cagtgtgtat
     1261 acattttacc tgacggatgc agaccatcat ttgcgatcaa tgctggatcg attgaagccc
     1321 gccgcgaaat cggtgtgatg tcgatatact gtgcgcccga tcggattgtc tctgctcgat
     1381 tgatcgcatt aaaccgatcg atctcggcgg cgatcttggc tcgagcgcgt ccggcggcga
     1441 acggcgtcac accccaatcg gggatcgaga gcacgatcac acgcgcaggc cgatcggcgg
     1501 caaaaccgat cgcgcgtttc aatagcgtgg caaattcgga ccgatagcca tccgctgagt
     1561 atccccgata ttgattgttc acgccaatca acaacgagac caaatcatac tggctcgacg
     1621 gttcgagctg atcgatcgcg gcattcagtt cgtccgtcgc ccagcccgtc ttcgcaatga
     1681 tgatcggctc atccagatcg atctgctccg cgcgcagcct cgacgtcagc tgcaccggcc
     1741 agcgttctgc ttccgccacg ctttc
//
LOCUS       DASRTJ010000296         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_244630,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000296 DASRTJ010000000
VERSION     DASRTJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244630"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1046
                     /gene="fdhD"
                     /locus_tag="VFF70_06010"
     CDS             279..1046
                     /gene="fdhD"
                     /locus_tag="VFF70_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZY44289.1"
                     /translation="MSDGVIKLSFDEYNHAWQQTEGEVIEEGFVSIFVNGAELATVMC
                     SPRDQDRLALGFLCNEGIINSIDDVRAIHVCPSGACVDVWLKRSDFVRPNRLILTSGC
                     GGGVTFDDLTKTIEPLNSDRLISPAKISALMNAMQQNAALYARARGVHTSALSDGEKL
                     IALAEDVGRHNTIDKLRGACLIEKIDPSGLILLCTGRISSEMINKVARMGCPIVASRT
                     SPTSLSVKLAREWNITLIGYLRRESMRVYAHPERLKN"
     gene            1136..1834
                     /locus_tag="VFF70_06015"
     CDS             1136..1834
                     /locus_tag="VFF70_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZY44290.1"
                     /translation="MDQTVTLLIDDQAVTVPGGTTIHKAAKSIGLDVPTVCYHDHTTS
                     NALCRVCVVDVEGQRLLQPGCIVQAADGMIVKTRSERVDRSRRTIIEMLGSAVDLSDA
                     PEIQAMAAEYHADFNRFAGERREQPVIDDNPMFIRDYSKCILCWRCVQVCADDAQYAF
                     AINFEGRGFDTSIGTFFDKPMPETTCVFCGQCVGVCPTGALKSKREYLLEQGNTPDQI
                     IAMTVMQRKRKRKG"
     gene            1838..3292
                     /locus_tag="VFF70_06020"
     CDS             1838..3292
                     /locus_tag="VFF70_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZY44291.1"
                     /translation="MNQPNEESLDPQDWDAMRALGHRMVEDMLTYLQTVRDRPAWQPM
                     PDRVKENLKKPLPIDPQSPEEAYRDFTENVLPYPLGNIHPRFWGWVIGTGTPFGVLAE
                     MLAATMNPNLGGGEHAANNVELQVLDWCKEILGFPLSASGLLVSGGSMANLVGLSVAR
                     NSMAGFDIRTQGVAAAPSRLVLYGSIEMHSSIEKAVELLGLGRDALRLIPTNDQFQID
                     LAALEKSIAVDRSAGYLPFCVIGNAGTVNTGAIDPLNDLADIAQREKMWFHIDGAFGA
                     LAAVSPDLRPLLKGLDRADSIAFDLHKWMYMPIEVGCVLVRDKETHRLAFTLTPEYLA
                     HYERGAASGAHWFNEYGPQLTRGFRSLKVWLSIKEHGIAKYGRVIKQNVDQCQYLTQL
                     IQASPDLELLAPTSLNIVCFRFKTAKSDDAALNHLNEKIMMELHEQGIAVPTYTILNG
                     KFAIRVAHTNHRTRREDFDLLVREVIRLGKELSA"
     gene            3289..4128
                     /locus_tag="VFF70_06025"
     CDS             3289..4128
                     /locus_tag="VFF70_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY44292.1"
                     /translation="MTSDLAPIVFGLLSAAAWGAGDFCGGLATKRSNVYGVLIGSQAA
                     GCVALVMMALAFSERMPAFDQMAWGGVAGLAGAFGLIALYTALATGRMSAAAPVSAVI
                     AALIPASFGILVQGVPNLLKLFGFGLALIGVWFVSSAKGSVIDLRQLQLPILAGLGFG
                     VFLIIINRVSDVSIFWPLVAARLAALIALLLVTTITQRPRLPDRTQLRLIVLAGLMDA
                     LGNSFFALAGRVGRLDVAAVLASLYPASTVALARLILKERITRMQSIGIAATLIAIVL
                     ITI"
     gene            4193..>5452
                     /locus_tag="VFF70_06030"
     CDS             4193..>5452
                     /locus_tag="VFF70_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY44293.1"
                     /translation="MLKPDRIVTTTCPYCGVGCNLELHIKDDTIFNVTSPFDSPVNHG
                     NLCVKGRFGYDYIYSDKRVTTPLIRKAPQTAGHRTQAFDRSEWREVSWDEALEYAADR
                     LVEIYRRDGSDAMAVYCCAKATNEDNYLLQKMYRTLFRTNNIDHCTRLCHAGSVMALI
                     KSLGTTAMSNTAAEVIKSDVFIVTGSNTTENHPIIGLQMKEAVEKYGAKLIVIDPRRI
                     ELCDYAVLWLPLKPGTNVPVFNAMAYVIIKEGLVNPKFIDERTVGYAEFERSIEKFTP
                     EYAEMLSGVDRNLIAEAARMYAQAKNGSIFWGMGISQLSSGTTSALGLIHLALLTGHV
                     GREGTGLNPLRGQNNVQGASDSGAMPFHFPGYMEVDKEENAVKWEKTWNIEPGGLSRK
                     RGLTTTEILTNAKPGGVRSLYIMGENPM"
BASE COUNT         1234 a         1344 c         1612 g         1262 t
ORIGIN      
        1 aatgtcaaag gtgagcttcc gataacgact gaggatactt ttcgaatcac tgtagatcga
       61 agcatggaaa atgtgatgaa gggacaagtt catggtgaat acccttattc tctacccatt
      121 gagtcgatcc cgtaattggc tggatgttat tcattccgtg caagccagcc ccacctagag
      181 cacgatccgg ttgaggcaga ggtaagcgct gcgcttagtg tccgtcgtgg aacaagagca
      241 cttactatac ttactaacta ctaatcattg aggtttctat gtctgatggt gtcatcaaac
      301 tttctttcga cgaatacaat cacgcctggc aacagaccga aggcgaagtg attgaagaag
      361 gcttcgtgtc gatctttgtc aacggcgccg aactggcgac ggtgatgtgc agcccacgtg
      421 atcaggatcg tttagcgctg ggtttcttgt gcaacgaagg catcatcaat tcgatcgatg
      481 acgtgcgcgc catccacgtt tgcccgtcgg gcgcgtgtgt cgatgtgtgg ctgaagcgat
      541 cggattttgt gcgcccgaat cgattgattc tcacatcggg ctgtggcggt ggcgtgacct
      601 ttgacgatct gactaaaacg attgagccgc tgaactccga tcggttgatc tcacctgcga
      661 aaatatcggc gttgatgaat gcgatgcagc agaacgcggc gttgtacgcg cgcgcccgcg
      721 gtgtccacac ttcggctctc tcggatggag agaaattgat cgcgctggcc gaagacgtcg
      781 gccggcacaa cacgattgac aaactgagag gcgcatgctt gatcgaaaag atcgatccaa
      841 gtggcttgat cttgttatgc accggacgca tctcgtccga gatgatcaat aaggtagcgc
      901 ggatgggctg cccgatcgtc gcctcgcgca cgtcgccgac atcattgtcc gtaaagttag
      961 cgcgtgaatg gaatatcacg ctgatcggtt atctgcggcg cgaatcgatg agagtgtatg
     1021 cgcatccaga gaggttaaag aattaggaca cggatttaac ggattgcacg gataaagcac
     1081 gaaaaataaa atcgtgttat ctgtgtacgt gtctgtgtcc agaatgagag tgaatatgga
     1141 tcagactgta actctattaa ttgatgacca agcagtgacc gtgccaggcg gtaccacgat
     1201 ccataaagcg gcaaaaagca tcggccttga cgtgccgacg gtgtgctatc acgaccatac
     1261 aacttcgaac gcgctgtgcc gcgtgtgtgt cgtcgacgtg gaaggccagc gcctcttgca
     1321 accaggttgc attgtccaag ccgctgatgg catgatagtc aaaacgcgat ccgagcgcgt
     1381 ggatcgatcg cggcgcacga tcatcgagat gctcggctcg gccgtcgatc tttcggatgc
     1441 gccggagatc caagcgatgg cggcggaata tcacgcggac ttcaatcgat tcgcaggtga
     1501 gaggcgcgag cagccggtca tcgatgacaa tccgatgttc atccgcgatt attctaaatg
     1561 tattttgtgc tggcgctgtg tgcaagtgtg cgccgatgat gcacaatatg cgtttgcgat
     1621 aaactttgag ggccgcggct ttgatacatc gatcggtaca ttttttgata agcccatgcc
     1681 ggagacgacg tgcgtattct gcggccaatg cgtaggcgta tgtccgaccg gcgcgctaaa
     1741 atcgaagcgc gaatatttat tggagcaggg caataccccg gatcaaatca tcgccatgac
     1801 ggtgatgcag cgtaaacgaa agcgaaaggg atgagccttg aatcaaccga atgaagagtc
     1861 actcgatccg caagattggg acgcgatgcg cgctttgggc catcgcatgg tcgaggacat
     1921 gctgacctat ttgcagaccg tgcgcgatcg accggcctgg cagccgatgc ccgatcgggt
     1981 taaagaaaat ttgaagaagc cgctgccgat cgatccacaa agccccgaag aggcttatcg
     2041 agattttacg gagaatgtct tgccgtatcc gctcggcaat attcatccgc gtttttgggg
     2101 ttgggtgatc ggcacgggca cgcccttcgg cgtgttggcg gaaatgctgg ccgcgacgat
     2161 gaatccgaat ttgggcggcg gcgaacacgc agccaacaat gtggaactgc aagtgcttga
     2221 ttggtgcaaa gagatcctgg gttttccatt gagcgcgagc ggcttgttgg tcagcggcgg
     2281 gtcgatggcg aacttggttg gcttaagcgt ggcgcgcaat tcaatggctg gttttgatat
     2341 tcgaacacaa ggcgtagccg ccgcgccaag tcgactggtg ttgtatggtt cgatcgagat
     2401 gcacagttcg atcgagaaag cagtggagtt gttaggcttg ggtcgtgatg cgctgcgact
     2461 gattccaacg aacgatcagt tccagatcga tctagcggcg ttggaaaaat cgatcgcggt
     2521 cgatcgatct gccggctatc tgccattctg cgtgattggc aatgcgggca cggtcaatac
     2581 cggcgcgatc gatccattga atgatctggc tgacatcgct caacgcgaaa aaatgtggtt
     2641 tcacatcgat ggcgcgttcg gcgcattggc ggcggtctca ccagatttgc gtccgttgct
     2701 gaaaggtttg gatcgcgcgg attcgatcgc attcgatctt cacaagtgga tgtatatgcc
     2761 gatcgaagtc ggctgcgtgc tggtgcgcga caaagagacg caccgcttgg cgttcacgtt
     2821 gacgccggaa tatctggcgc actacgaacg cggcgcggcg agcggcgcgc attggttcaa
     2881 cgaatacggg ccgcagttga cgcgcggctt ccggtctttg aaagtgtggc tatcgatcaa
     2941 ggagcacggc attgccaagt atgggcgcgt gatcaaacaa aatgtcgatc agtgccaata
     3001 tttgacgcaa ttgattcaag cgtcgcctga ccttgaattg ctcgcaccga cttcactcaa
     3061 tatcgtttgt ttccgtttca agacggccaa gagcgatgat gctgcactga atcatctgaa
     3121 cgaaaagatc atgatggagt tgcacgagca gggtattgcc gtgccgacgt ataccatcct
     3181 caacggcaaa tttgcgattc gcgtcgcgca cacgaatcat cgcacgcggc gtgaagattt
     3241 cgatctactg gtgcgtgaag tgatccgcct tggcaaagaa ctttcggcat gacgtccgat
     3301 ctcgcgccga tcgtgttcgg cttgctatcg gccgctgcgt ggggtgcggg tgatttctgc
     3361 ggcggattgg ccacgaaacg ctcgaatgtt tatggcgtgc tgatcggctc acaagccgca
     3421 ggctgcgttg ccctcgtcat gatggcactg gcgttctcag aacgcatgcc tgcgtttgat
     3481 caaatggcgt ggggcggtgt cgccggactg gccggtgcgt ttggattgat tgcactctat
     3541 actgccttag caacgggtcg aatgagcgca gctgctccgg tctctgcggt gatcgcggcg
     3601 ctgatcccgg caagctttgg cattctggtg cagggtgtgc cgaatctatt gaagttgttc
     3661 ggttttgggt tggccttgat cggtgtgtgg tttgtctcga gcgcgaaagg ctcagtgatc
     3721 gatcttaggc aactgcagct gccgatcttg gcgggtctgg gcttcggcgt cttcttgatt
     3781 attatcaatc gagtcagcga cgtttcgatc ttctggccat tggtggcggc gcggttggcg
     3841 gctttgatcg cgttgctgct cgttacgaca atcacccaac ggccgcgctt acccgatcgg
     3901 acgcagctgc gcttgatcgt tttagccggc ctcatggatg cactcggtaa ttcgttcttc
     3961 gcgttggcgg gccgagtcgg tcgactagat gtggcggcag tgttagcctc attatatcca
     4021 gcctcaaccg tggcgctggc aaggttgatc ttgaaagagc gcatcacacg gatgcaatcg
     4081 atcgggatcg ccgcgacgtt gatcgcgatt gtattgatta cgatttagag tgggtctctg
     4141 gatgaagcga agtgaagtct attcggattg agaaatagga taggagcctc agatgcttaa
     4201 acccgatcga attgtcacca ctacatgtcc atactgcggc gtaggctgca acctcgaatt
     4261 gcacatcaaa gacgacacca ttttcaatgt gacttcgccg ttcgatagtc cggtcaatca
     4321 tggcaactta tgcgtcaaag gccgcttcgg ctacgactac atttacagcg acaagcgggt
     4381 gaccacgccg ttgattcgca aggcaccgca gacagccgga caccgcacgc aagccttcga
     4441 tcgatccgag tggcgcgaag tctcatggga cgaagcgtta gagtatgcag ccgatcggct
     4501 agtggaaatt tatcggcgcg atggatcgga tgcgatggcg gtgtattgct gcgccaaagc
     4561 gacgaatgaa gacaattatc ttttgcagaa gatgtatcgc accttgttcc gcaccaacaa
     4621 catcgatcac tgcacacgct tgtgccacgc cggatcggtg atggcgttga tcaagtcact
     4681 gggcaccacg gcgatgagca acaccgccgc cgaagtgatc aagagcgatg tattcatcgt
     4741 caccggctcg aacaccaccg agaatcaccc aatcatcggc ttgcagatga aagaggctgt
     4801 cgaaaaatat ggcgcgaagt tgatcgtgat tgatccgcgc cgcatcgagt tgtgcgatta
     4861 cgcggtgttg tggctgccgt tgaaacccgg cacgaacgtg cccgtcttta atgcgatggc
     4921 gtatgtgatt atcaaagagg gtttggtcaa tccgaagttt attgacgaac ggacagtcgg
     4981 ctatgcggag tttgaaagat cgatcgagaa gttcacgccc gaatatgccg aaatgctcag
     5041 cggcgtcgat cgaaatctga tcgccgaagc ggcgcgcatg tatgcgcagg ccaagaacgg
     5101 atcgatcttt tgggggatgg gcatctcgca actatcgagc ggcacgacca gcgcgttagg
     5161 cttgatccat ctggcgttgc tcaccggtca cgtcgggcgt gaaggcacgg gtctcaatcc
     5221 attgcgcgga caaaacaatg tgcagggcgc atcggattcg ggcgcaatgc cgtttcattt
     5281 tcccggctac atggaagtgg acaaagaaga gaacgccgtc aagtgggaaa aaacatggaa
     5341 catcgaaccg ggcgggctca gccgcaagcg cggcttgacc acaactgaaa ttttgacaaa
     5401 cgcgaaaccg ggcggcgttc gatcgttgta catcatgggc gagaatccaa tg
//
LOCUS       DASRTJ010000297         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_348191,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000297 DASRTJ010000000
VERSION     DASRTJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348191"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFF70_06035"
     CDS             complement(<1..910)
                     /locus_tag="VFF70_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZY44294.1"
                     /translation="MRVLITGGAGFIGCNAADRFLRRGDQVTIFDNLSRRGVNSNLVW
                     LRTRHGPDSFRLTQADVRDAASLLNAARDQEVIIHLAGQVTVTTSVIDPRSDFETNAV
                     GTFNALEAARLSGSNPIFLYSSTNKVYGGMDDVVLTEGAQRYSYRDYPLGIPETYPLD
                     FHSPYGCSKGTGDQYVRDYARIYGLRSVVLRQSAIYGPRQFGIEDQGWLAWFIIAAVS
                     GKLITIYGDGKQVRDVLFIDDLIDLYETAITKIDQSAGRVFNVGGGADNILSIWTDFG
                     PMLEKLVDRKIDVGRGDWRPGDQRIYV"
     gene            complement(907..>1572)
                     /locus_tag="VFF70_06040"
     CDS             complement(907..>1572)
                     /locus_tag="VFF70_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZY44295.1"
                     /translation="STIIVEHVLDVFTVLGILVLLLPFLPLPDWAARGATVSAYVFGA
                     ALLVMLLMVWQRTRIEHWAEQILDRIPRLHTATWLQHVIHILDGFAVLQPGKPLIAAV
                     TLSVVGWLASAATIHLALMAFVPNPDPIVSFFVTVTTTFSLLLPATPGGIGVLQGAIV
                     LSLAVFNIPQAPALSFAIVFHLMEIIVMDVLGAIALWREAGSWAGMKAQIRSVTTAAE
                     SQP"
BASE COUNT          413 a          454 c          407 g          298 t
ORIGIN      
        1 acacgtagat gcgctgatcg ccgggacgcc aatcgccacg accgacatcg atttttcgat
       61 ccactaattt ttccaacatc ggaccgaaat ccgtccagat cgataggata ttgtccgcac
      121 cgccgccgac gttgaagact cgacccgcag actgatcgat cttagtgatt gctgtctcgt
      181 acaaatcaat taagtcatca ataaacagta catcgcgaac ctgtttgcca tcgccgtaga
      241 tcgtgatcag tttaccactg actgctgcaa tgatgaacca cgccagccaa ccttgatctt
      301 cgatgccgaa ttgacgcggg ccatagatcg ctgattggcg caagacaacc gatcgcaggc
      361 catagatgcg cgcgtaatcg cgcacatact gatcgcctgt gcctttactg cagccatacg
      421 gcgaatgaaa atcgagcggg taagtttcgg gaatgcccag cggataatcg cggtacgaat
      481 aacgctgtgc gccttcggtc agcacgacat catccatacc gccgtaaact ttgttggtgg
      541 atgaatagag gaagattgga ttagaaccgg ataatcgagc agcttccagt gcgttgaaag
      601 tgccaacagc attcgtttca aaatcgctgc gcggatcgat caccgaagtg gtcacagtca
      661 cctgtccggc gagatggatg attacttctt gatcgcgcgc cgcattgagc agtgaagccg
      721 catcccgcac atcggcttgc gtcaatcgaa aggaatcggg gccgtgtcgc gtgcgaagcc
      781 aaacgagatt ggagttgact ccgcgccgcg ataagttatc gaagatggta acttgatcgc
      841 ctcgccgcag aaatcgatcg gctgcattgc aaccgataaa acccgcgcca cccgtaatca
      901 atactctcat ggttgactct ctgccgccgt cgtcacggat cgaatttgcg ctttcatccc
      961 ggcccacgaa ccggcttcgc gccacaaggc aatcgcgccg aggacatcca tcacaatgat
     1021 ctccatcaga tggaacacga ttgcaaaact caacgcgggc gcttgaggaa tgttgaacac
     1081 cgcgagcgat aaaacgatcg cgccctgcaa gacgccgatg ccgcccggtg tggccggcaa
     1141 taacaatgag aacgtagtcg tgaccgtcac aaagaacgac acgatcggat caggattagg
     1201 cacaaaggcc atcaacgcca gatggatcgt cgccgccgag gccagccagc ccaccactga
     1261 taaagtcacg gcggcaatta acggcttgcc cggttgcagc accgcaaagc catccaagat
     1321 gtgaataaca tgctgcagcc aagtggcggt gtgcaggcgc ggaatccgat cgagaatctg
     1381 ctcagcccaa tgctcgatgc gcgtgcgctg ccagaccatc aacaacatca cgagcagcgc
     1441 cgcgccgaac acatacgcgg agacggtggc accgcgcgca gcccaatcag gtaatggcag
     1501 gaatggcagc aacagcacta aaatgcccag cacggtgaat acgtccaaca cgtgttcgac
     1561 aataatcgtc ga
//
LOCUS       DASRTJ010000298         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_55869,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000298 DASRTJ010000000
VERSION     DASRTJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55869"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /gene="thrC"
                     /locus_tag="VFF70_06045"
     CDS             complement(<1..1453)
                     /gene="thrC"
                     /locus_tag="VFF70_06045"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZY44296.1"
                     /translation="MLDPVLECLRCGERFAFTRLIPVCPKCGHDLLEIRYDYAALAEE
                     LPALLRERPFNMWRYRELLPTRHTANIVSMGEGGTPLIEANNLATMLGHDNIFIKDER
                     QGPTGSFKDRQAALAISAMKEAGTLEAVVASTGNVAISYSAYSARAGIKLWAFLTSLV
                     PADKMREVALYGTEVVKVTGTYDQAKEVAAAFAEHRGLYMDRGIRSIASRQSMKTLAY
                     EVCEQLGWLKDGWGKFHAPDWYVQAVSGGTGPLGAWRGFEELYQMGFIDRLPKIAVIQ
                     AEGCAPMVSAFKAGRTETDIVSQPNTHIATLATGNPGIAYTQLRQIILEHGGCMESVT
                     DDESFRAMHVMAKMDGISIEPATAAAFAGLFKLIGLQVIKRAEIVVVNCSGHTFPVEK
                     ELLGDDWMRNFELPQITSETTPAIPVSNVPQEGLLSALELLDDHMRDILIIDDTPEAV
                     VLLRRILQAHGAFAVREANSGRVGLEMARAATPD"
     gene            complement(1481..>3592)
                     /locus_tag="VFF70_06050"
     CDS             complement(1481..>3592)
                     /locus_tag="VFF70_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44297.1"
                     /translation="RQYRTLAAQAATVLENRRLFENVEARVNELSVLTRIGRRIASTL
                     DLHEVLSLIVEETIGTTKATQASIALYNETENALEVRVMRGYAPEIETNTLGMLIQPG
                     QGLHGRLLITGQSVLMNDVRIDPDYMDVNSDTRSEFIIPIRQGDLLLGALNLESPTVS
                     AFDEADRRLIEALADQAAVAITNARSYEAERKAVERMREVDRLKTQFLANMSHELRTP
                     LNSIIGFSRVIMRGIDGPLTELQMTDLQSIYNSGQHLLGLINNILDISKIEAGKMELA
                     NEPIDLRDIARGVMSTVSALIKDKSVKLEQEIPDNLPPITADSTRVRQIMLNLMSNAA
                     KFTEQGKITLRVVTNPREVQVSVIDTGIGIPANKLEHIFEEFTQVDASTTRRVGGTGL
                     GLAITRRFVEMHGGRIWVESQLSVGSTFTFTLPLHIPEEPPERVSLPIDLAARDAGKK
                     VILSIDDDPGVITLYKRYLDKQGYLVIGLTDPSKAVDEAKRLLPFAITLDIMMPSRDG
                     WSLLADLKSTPEINRVPIVVCSILQDKTKGYSLGATEYLVKPITEDELLHALDRVSRH
                     NIIRNVLVIDDEPTGIQLIKRILEVNGNYQILEASGGAEGIARVEFDRPDLIILDLMM
                     PDVDGFTVLETIKSSAATRRIPVIVVTAKEITREDRDRLNGHAVALFNKGMFTADQLL
                     TELIGALKQLNGAEVVNPDTK"
BASE COUNT          766 a          996 c          982 g          848 t
ORIGIN      
        1 aatcgggagt tgcagcgcgc gccatttcca atccgactcg accggagttt gcttcacgca
       61 cagcgaaggc gccgtgcgct tgcaggatgc gacgcagcaa gacgaccgcc tcaggtgtat
      121 cgtcgatgat caaaatgtcg cgcatgtgat cgtccaacaa ctccaaagcc gacagcagcc
      181 cttcttgcgg aacattgctg accgggatgg ccggtgttgt ctcggatgta atctgtggta
      241 gttcaaagtt gcgcatccaa tcatcgccca gcaattcttt ttcgactgga aaagtgtggc
      301 cggagcagtt gaccacgacg atctcagcgc gtttgatcac ttgcaagccg atcaatttaa
      361 atagtccggc gaaggcggcg gccgtcgccg gttcgatcga gatgccatcc atcttcgcca
      421 tgacatgcat ggcgcggaag gattcatcat cagttacgct ttccatgcag ccgccatgtt
      481 cgagaatgat ctggcgcagc tgcgtataag cgataccagg attgccggtg gcgagcgtgg
      541 cgatatgcgt gttaggctgg gagacgatgt ccgtttcagt gcgaccggct ttgaacgcgc
      601 tgaccatggg cgcgcagccc tcggcttgaa tcacggcgat cttcggcagc cgatcgatga
      661 agcccatctg atagagttct tcaaagccgc gccacgcgcc gagcggcccc gtgccgccgc
      721 tgacggcctg cacataccaa tcgggcgcgt ggaatttgcc ccaaccatct ttcagccagc
      781 cgagctgctc acacacttca tacgcgagtg tcttcatgct ctgccgcgac gcgatcgatc
      841 ggataccgcg atccatatat aggccgcgat gttcggcaaa ggcggctgcg acctctttcg
      901 cttgatcata agtgccggtg actttgacga cttcagtgcc gtacagcgcg acttcgcgca
      961 tcttatcggc gggaactaaa ctggtgagga acgcccacaa cttgataccg gcgcgcgcgc
     1021 tgtacgccga gtacgagatg gcgacattgc cggtggaggc aacgaccgct tccagcgttc
     1081 cggcttcttt catggcgctg atcgccaacg cagcctgtcg atctttgaat gaacccgtcg
     1141 gtccctggcg ttcatctttg ataaagatgt tgtcgtggcc gagcatcgtc gccagattgt
     1201 tggcttcgat cagcggcgtg ccaccttcac ccatgctgac gatattggct gtatgccggg
     1261 tgggcagcag ctcgcgatag cgccacatat tgaagggacg ttcgcgcaac aacgcgggca
     1321 gctcttcagc taaagctgca tagtcatacc tgatttccag cagatcgtgt ccgcactttg
     1381 gacacacagg gatcaaacgg gtgaaagcga agcgttcacc gcagcgtaag cattccagaa
     1441 caggatcaag catagtgatt tttataccat ttagctggga ttattttgtg tccgggttga
     1501 ccacctctgc cccgttcaac tgtttgagtg cgccgatgag ttcggtcaat aattgatcgg
     1561 ccgtgaacat gcctttattg aagagcgcca ccgcatgccc gttcaaccga tcgcgatctt
     1621 cgcgcgtaat ttctttggcg gtcacgacga tcacaggaat acgccgcgtt gcggcgctgc
     1681 tcttgatcgt ttccagcacc gtgaaaccat ccacgtcagg catcatcaga tcaagaatga
     1741 tcagatcggg ccgatcgaat tccacgcggg caatgccttc cgcgccgccg gaggcttcca
     1801 gaatttgata attgccattc acttcgagaa tgcgcttgat caattgaatc ccagtcggtt
     1861 catcatcaat cacgagtaca ttccggatta tgttgtggcg gctgactcga tcgagcgcgt
     1921 gcaacagctc gtcttcagta atcggcttga ccagatattc ggttgcgccc aatgaatagc
     1981 ctttggtttt atcttgtagg atgctgcaca ccacaatcgg cacacgattg atttcaggcg
     2041 tgcttttgag atcggccagc agcgaccagc catcgcgact gggcatcata atgtcaagcg
     2101 tgatagcgaa tggcagcaag cgtttggctt cgtcgacggc tttgctcgga tcggtcaggc
     2161 cgatcaccag ataaccttgt ttatctaaat agcgtttata caaggtgatg acgccaggat
     2221 cgtcgtcgat actgaggatc actttcttgc ccgcatcccg cgccgcgaga tcgatcggca
     2281 ggctaacgcg ctccggtggt tcttccggga tatgaagtgg cagcgtgaac gtaaatgtgg
     2341 agccaacgct caactgcgat tcaacccaaa ttcgcccgcc atgcatttcg acaaagcggc
     2401 gagtgatggc cagacccaga cctgtgccgc cgacgcgccg cgtggtactc gcgtcgactt
     2461 gagtgaactc ttcaaagata tgttccagct tgttggccgg aataccaatc ccggtatcga
     2521 tgactgaaac ttgtacttcg cgtgggttgg tgacaacccg gagcgtgatc ttgccttgtt
     2581 cggtaaactt ggcggcgttg gacatcaggt tcagcataat ctggcgcaca cgagtggaat
     2641 cggcagtgat gggcggtaag ttatctggaa tctcttgctc aagtttgacc gacttgtcct
     2701 tgatcagcgc actgacggtg gacatgaccc cgcgcgcaat gtcgcgtaga tcgatcggtt
     2761 cgtttgccaa ttccatcttg ccggcttcga tcttggatat atccaagatg ttgttgatca
     2821 agcccagcaa atgctggccg gagttgtaga tcgattgcag gtcggtcatc tgcaattcgg
     2881 tcagcggtcc atcgatgccg cgcatgatca cgcggctgaa gccgatgatg gagttgagcg
     2941 gcgtacgcag ctcgtggctc atattcgcca agaactgagt cttcagccga tcgacttcgc
     3001 gcatacgctc cactgctttc cgttccgctt catacgatcg ggcgttcgtg atggcgaccg
     3061 cggcttgatc ggctaacgcc tcaatcaagc gtcgatcggc ttcatcgaac gcgctgactg
     3121 tcgggctttc cagattcaac gcgcctaaca atagatcgcc ttgacgaatg ggtatgatga
     3181 attccgaccg agtatcgctg ttgacatcca tataatccgg atcgatccga acgtcattca
     3241 tcaaaaccga ttggccggtg atcaacaatc gaccgtgcaa gccctggcca ggttgaataa
     3301 gcatgcctaa cgtattcgtt tcaatctctg gcgcatagcc gcgcatcact ctgacttcca
     3361 atgcattttc agtttcgttg tagagcgcga tactggcctg cgtggctttg gtggtgccga
     3421 tggtttcctc gacaattaaa ctcaacactt catggaggtc aagggttgaa gcgatacgcc
     3481 ggccgatgcg ggtcagcacc gaaagctcgt tgacgcgggc ttccacattt tcaaacagcc
     3541 gccgattctc aagtactgtc gcggcctgcg ccgccaacgt gcggtattgg cg
//
LOCUS       DASRTJ010000299         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_348198,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000299 DASRTJ010000000
VERSION     DASRTJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348198"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VFF70_06055"
     CDS             complement(<1..1593)
                     /locus_tag="VFF70_06055"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZY44298.1"
                     /translation="MSTIRKITLLFSISIVSVVLLATRSGPSLAANPPANPAAPSASL
                     TLLAQQDAYIDESLPTTNYGGEDSLWVGRVSSQLRAPFDLHTLVWFELSALPPGASIT
                     AAKLQLYEISATLQSTYAITPDIVLRPWDEMLVTWNNAPPHSNLGDAAVAVDMTNGWK
                     TWDVTNIVKAWLLPNTNYLNYGIRLLGDGSTIGRRQFGSRNDPQLAPRLIVRYTTNPK
                     PTRTPVPTWTPIPTAQPTVGPSPTPTPVFPLFTYYLSPPIFQYYPPTIDLSIAGIELT
                     EGIQCFDTSKGLAGCANNSLPLISKKNATARIYLKYTGSGSSLNNVPVRLHLRANNVW
                     YVVNGSGQARSTLDQSNANYSVNVWFNVDFNSDVNVDYWAEVDPNHMIAETNESNNCY
                     PTPSTCSDSSYSTLLFHKRGTLNVVGQRIRYHPSGYAGNQYAGGWAVNGGGATWWNAV
                     LPLRTNGINYSLNSGYLDWTTPLDGSGQHALISTLNLLWIQQNALSWWFGTGSFTGAR
                     HVYGWAPSAGYSGGHADMPVYPH"
     gene            2062..2547
                     /locus_tag="VFF70_06060"
     CDS             2062..2547
                     /locus_tag="VFF70_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44299.1"
                     /translation="MKAKSKVKAKPATKAKSALTAKPKVATKQGDGTREKPWQLKTAS
                     GSSEYMMYRDESANPPALMCLVGSTTLSYQLRSLDDLQAMLKRHGDWMLLGSADEQKP
                     AADGTVEAWGRSASNPVGGWYGLKKGLRGRFGMYLPPLMELLGLAEVEHNAKNNRMRA
                     K"
     gene            2706..3623
                     /locus_tag="VFF70_06065"
     CDS             2706..3623
                     /locus_tag="VFF70_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44300.1"
                     /translation="MPPDPSSQTIIVQLNQNAGFWSNVNRAVNRLALSIGRNGIAALR
                     IDWRIDPANQVSPYGTAADGNLWEKYFEPIEFESFPATETIVSSYADRQMTGLRAYYM
                     YKTDRNWRQRYHTAYTRYIRLKPQVQQQVTAVYERQMAGKFCVGVHFRNSFHGSLECP
                     EPNLPLDDYLSRTRAQLADRSGGVLVLATDVAEAVDRFHAEFGDQLIYQTQVLRSRRD
                     GDHEVFGRHQSPSVQWGLDVLIDCLLLAKCNSLLHVTSNIATAAGYINPNLKMIYCER
                     SPVLGMLHYWQKLGNHLLTPRLKRVFSAR"
     gene            3827..5044
                     /locus_tag="VFF70_06070"
     CDS             3827..5044
                     /locus_tag="VFF70_06070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY44301.1"
                     /translation="MALDKQNLYRLPWSKNDNPIGWLEVTDICNLKCLGCYRQKISGD
                     KTLDQIKEEVLFLKRWRKVDNISIAGGEPLMHPQIMDVISFIQQQDIKPIMLTNALKL
                     DRPTLVELKKAGLAGFTIHIDSNQGRPQWRDKTEVETNDLRQHYADLIHDVGGLYLVF
                     NSTVYPSTEKYIPEMVQWAQRNIDRVNGMVFICFRTAPLEEEITYQVGDELIDARHLS
                     YASESPAERDITSYEVHDLIKENNPNFDAAGYLGGTWKIDSIKWLAGAMIGNKHQMYG
                     SIGPKTMELAQTGHHLFKGRYLAYLSDGKVGGKIFWLSAFDRKVRSAYKNFWGDVLRH
                     PTRLFNPIYLQTIGVIQAPDIMADGTADMCDSCPDMTYWNGELVNSCRMDEYRLFGGF
                     VTVVNREEAAQVR"
BASE COUNT         1259 a         1332 c         1440 g         1221 t
ORIGIN      
        1 atgcggatag accggcatgt ctgcatgccc gccgctgtaa ccggcgctgg gcgcccatcc
       61 gtagacgtgg cgtgcgccgg tgaagctgcc ggtgccaaac caccagctca acgcattttg
      121 ctgaatccat aaaagattca acgtactgat caacgcatgt tgaccggagc catccaatgg
      181 cgtggtccaa tccaaatagc cgctgttgag cgagtagttg atgccattgg tacgcagtgg
      241 caggactgcg ttccaccaag tcgcgccgcc gccattcacc gcccaaccgc cggcatactg
      301 attgccggca tagccgctgg gatgataacg tatacgttgc ccgaccacat tcagcgtgcc
      361 gcgtttgtgg aaaagcagcg tggagtaact tgaatctgaa caagtgctcg gggtgggata
      421 gcaattgttg ctttcatttg tttcggcaat catatgattg ggatcaacct cagcccagta
      481 gtcgacgttc acatcgctgt tgaaatcgac attgaaccag acattgacgc tgtagttcgc
      541 gttcgattga tcgagcgtcg atcgcgcctg gccgctgcca ttcaccacat accagacatt
      601 attcgcgcgc agatgcagcc gcaccggcac attgttcaga ctgctgccgg agccggtgta
      661 tttcaaatag atgcgcgccg tcgcattttt cttggagatg agtggcagtg agttgttagc
      721 gcagcccgcc agccctttgc tggtatcgaa gcattgaatg ccttcggtca gctcaatacc
      781 ggcgatcgat agatcgatcg tgggcggata atattgaaag attggcggcg acaggtaata
      841 agtgaagagc ggaaagactg gcgtcggcgt gggcgatgga cccactgtcg gctgggcagt
      901 gggtatcggt gtccaggttg ggacgggcgt gcgggttggt tttggattcg tggtgtagcg
      961 cacgatcaat ctgggtgcta actgtggatc gttacgcgaa ccgaattgcc gtcgcccgat
     1021 cgtgcttcca tcgccaagca agcggatgcc gtaattcagg taattcgtat tgggaagcag
     1081 ccaggccttg acgatgttcg tcacatccca cgtcttccag ccatttgtca tgtcgacggc
     1141 cacggctgca tcgccgagat tggaatgggg cggcgcatta ttccaagtga cgagcatttc
     1201 atcccatggc cgaagcacaa tgtcgggcgt gatggcataa gtgctctgca gtgtggctga
     1261 gatttcgtaa agctgcagct tggcggccgt gatactggcg cccggcggca gcgccgaaag
     1321 ttcgaaccag accaaagtat gaagatcaaa tggcgcgcga agctgggaac taaccctgcc
     1381 cacccataag ctatcctcgc cgccatagtt tgttgttggc agactttcat ctatataagc
     1441 gtcttgttgc gccagcagag tcaggctggc ggagggggcg gccggatttg caggggggtt
     1501 agccgctaag gacggccccg accgcgtggc aagtaagacc acgctaacga tactgattga
     1561 aaagagtaga gtgatttttc gaatagtaga catgaccatc tccttatcat gattcgccta
     1621 acccgttaga attgtctgag catgatgagt gagaaataac gggttcggct gccgacctct
     1681 gacttgatcg cgaaatggag ggtatcttac gggcgtacgg cagttgttaa atagtatagg
     1741 ctcaagcgtg gtctgctgtc aacattttcc attttttcac gagtctgtta aattcaaatt
     1801 gatttcccgg cttgtccatg aaattcttgc ttctctgtct gatcgatcca attgatttgc
     1861 agaatcaaaa tttttcgtca cttcgtaaat cttattttct gttggcagta ttgcaaccag
     1921 attcccaaag gtttaatgtc gggtagcggc ttccacttgg caattaatgc attgctactg
     1981 tagccaaccg gctaaacttg attcgatgat tgatctatgt taacattcag tcagtccagt
     2041 caaattgtga gaggagacaa aatgaaagcg aaatcgaaag tcaaagcaaa gcccgcaaca
     2101 aaagcgaaat cggcattgac cgctaagcca aaagtggcga cgaaacaagg cgatggtaca
     2161 cgcgagaagc cgtggcagct aaaaacagcg agtggttcat cggaatacat gatgtatcgc
     2221 gatgagtcgg ccaatccgcc tgccttgatg tgcctcgtcg gctcgaccac cctcagttat
     2281 caattgcgca gcctcgacga tctgcaggcg atgctcaagc ggcacggcga ttggatgttg
     2341 ctcggcagcg ccgatgagca gaagccggcc gccgatggca ccgttgaagc gtggggccga
     2401 tcggccagta atcccgtcgg cggttggtat ggattgaaga aaggtttgcg tggtcgcttc
     2461 ggcatgtacc tgccgccctt gatggagttg ttgggcttgg ctgaagtcga acacaatgcc
     2521 aagaacaatc ggatgcgcgc gaagtgatcg attagctgat ctcccccaga aatcgatcgg
     2581 ccccttcgag ttttgcgaag cgcccccgca agcgcagcgg agtggggcgg aaactcgaag
     2641 ggtttttatt tatcaagact atcgcttaac attcgaatag acagttttag tgcaggcgtt
     2701 aaagaatgcc acctgatcca tcttcgcaga cgatcattgt ccaactgaat caaaatgccg
     2761 gcttctggtc gaatgtcaat cgggctgtca accgcttagc cctttcaatc gggcgaaatg
     2821 gaattgcagc tctgcggatc gattggcgga tcgatccagc aaaccaagtt tcaccctatg
     2881 gcacggccgc cgatggcaat ctgtgggaaa aatattttga gccgatcgag tttgaatcgt
     2941 ttccggcaac cgaaacgatt gtttcaagct atgcagatcg gcagatgacc ggactcagag
     3001 cgtattacat gtacaaaaca gatcgcaact ggcgacagcg ttatcacaca gcttatacgc
     3061 gctacatacg ccttaagccg caggttcagc aacaagtgac ggccgtttat gagcggcaga
     3121 tggctggaaa gttttgtgtg ggggtgcact ttcgcaactc atttcacgga tcgttagaat
     3181 gtccagagcc taatctgcct ttggatgact atctgtcccg cacgcgtgcg cagctagcgg
     3241 atcgatcggg cggcgtgctg gttctggcca ccgatgtagc cgaagctgtt gatcgttttc
     3301 acgccgaatt tggcgaccag ctgatttatc agacgcaggt gctgcgctcc cgacgtgatg
     3361 gcgatcacga agtatttggc cgacatcaat caccgagtgt ccaatggggt ctggatgttt
     3421 tgatcgattg cctgttactg gctaaatgca acagcttatt gcatgtcacc tcaaacatcg
     3481 ccaccgcggc cggctatatc aatccgaacc taaaaatgat ttactgtgaa cgttcgcccg
     3541 tactggggat gctgcactac tggcaaaaat taggcaacca cctcctcaca ccgcgactca
     3601 agcgggtatt ctcggcgcgt tgacgcgttt tgttgataga cgacttcaat atcgcattta
     3661 tcttcgttag atatccgcgc tcgcttgatt aacccgttcc cggtcacact aattctctca
     3721 aagcgaataa attgcgcaag agtgcatgac atttattact gccaagcctg tcgcgcttgc
     3781 gctaagatga ccgtttaagc atctatccgc atggcaggag atcagcatgg cactagacaa
     3841 gcaaaatctg tatcgtttac cgtggtccaa gaacgacaac ccgatcggct ggctggaggt
     3901 gaccgacatc tgcaacctga aatgtctggg ctgctaccgg caaaaaatct ccggcgacaa
     3961 aacgctcgat cagatcaaag aagaagtcct ctttctcaaa cgctggcgca aagtcgataa
     4021 tatttcgatc gcgggcggcg aaccgttgat gcatccgcag atcatggacg tgatcagttt
     4081 catccagcag caggacatca agccgatcat gttgacgaac gcgctgaaac tggatcggcc
     4141 cacgctagtt gaactgaaga aagcggggct ggcgggtttc accattcaca tcgacagcaa
     4201 tcagggccgc ccgcagtggc gcgacaagac cgaagtggag accaacgatc tgcgccaaca
     4261 ttatgccgat ctgatccacg acgtcggcgg gctatacctg gtctttaaca gtaccgtata
     4321 tccctcgacc gaaaagtata ttccagagat ggtgcagtgg gcacagcgca acatcgatcg
     4381 agtcaacggc atggtcttca tctgcttccg caccgcgccg ctggaagaag agatcaccta
     4441 tcaagtcggc gatgaactga tcgacgcgcg gcatttgagt tacgccagcg agtcgcccgc
     4501 cgagcgcgac atcacttcgt atgaagtgca tgatctgatc aaagaaaaca atccgaattt
     4561 tgacgcggcg ggttatctgg gcggtacctg gaagatcgat tcgatcaaat ggctggcggg
     4621 cgcgatgatc ggcaataagc atcagatgta cggctcgatc ggtccgaaga cgatggagtt
     4681 ggcgcagacc ggccatcact tgttcaaggg gcgttacctt gcgtacttga gcgatggaaa
     4741 agtcggcggc aagattttct ggctgtcggc gttcgatcga aaagtgcgtt ccgcttacaa
     4801 aaatttctgg ggagatgtgt tgcgccatcc cacgcgcttg ttcaatccga tctacttgca
     4861 gacgatcggc gtgattcaag cgccggatat catggcggac ggtacggccg acatgtgtga
     4921 cagctgcccc gatatgacct actggaatgg cgagttagtc aactcctgcc gtatggacga
     4981 gtatcgattg ttcggcggct ttgtgacggt ggtcaatcgc gaagaagcag cacaggtgcg
     5041 ctaacgatcc ggcggcaaga aaagattcgc ctggacacgt atggaatgcc tatatttttc
     5101 tcgtcgcggt aaaaatgttg tatacttcgc caggagcaag gcaaggctgc atgattacca
     5161 ttcgcatggt tggcacgcag tcgatcaaca ccgaaccgta ttcatgcctt gcgcttggag
     5221 caaaccatgg actacaccca gtatccccgt cc
//
LOCUS       DASRTJ010000300         1582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93119,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000300 DASRTJ010000000
VERSION     DASRTJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93119"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1582)
                     /locus_tag="VFF70_06075"
     CDS             complement(<1..>1582)
                     /locus_tag="VFF70_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZY44302.1"
                     /translation="SADGVSVSPPAVAASINPVQGEDVVPAKAVRIKPTRIDQAPITA
                     TQLPLTVMKTVVGLARTIEPKIEPAKPKLIRERIMPALDLLEQHTASTIEQQEIDRKS
                     QVIEQTLKQFGLEAEVVNTAQGPAVTQFQVAPGYIDRPGPDGVARKQKVRVGQISSLS
                     NDLALALAAQSLRIEAPVPGKTFVGIEVPNDQIAMVGLRGVLESEDFKKIGEPLAFAL
                     GRDVSGGAIAADLASMPHVLIAGTTGSGKSVCINAIIMSLIMNNTPEELKLVMIDPKM
                     VELIRFNGLPHLYGKVESDMDRIVNVLRWIAREMDGRYKKFVEVSARHVNDYNTTMTE
                     QGGERLPYIAVLVDELSDLMMSAPVEIEKTISRIAQMARATGIHLVIATQRPSTDVVT
                     GLIKANFPARISFAVASNVDSRVILDSVGAETLLGKGDMLFLSPTANAPLRVQGCYVG
                     EREVDGVVKFWHDRYADETPEPAPWEMSNVSRLEEIASASPSAAKMENDDEVLLAKAI
                     ELVKRQRSASASLLQRKMR"
BASE COUNT          312 a          515 c          401 g          354 t
ORIGIN      
        1 cgcatcttgc gctgcagcag cgaggcgctg gcgctgcgct ggcgcttcac caactcgatc
       61 gccttcgcca gcaacacttc atcatcgttt tccattttcg ccgcgcttgg gctcgctgaa
      121 gcgatctctt ccaagcggct gacattgctc atttcccacg gcgcgggttc gggcgtttca
      181 tctgcgtatc gatcgtgcca gaacttcacc acgccatcaa cttcgcgttc gccgacatag
      241 caaccctgca cgcgcagcgg cgcattcgcc gtcggcgaca agaataacat atcgcctttg
      301 cccaacaacg tttccgcgcc gaccgaatcc aaaatcacgc ggctgtccac attcgatgcg
      361 accgcaaacg aaatgcgcgc cggaaagttc gctttgatca aaccggtgac cacatcggtc
      421 gatgggcgct gcgtggcgat gaccaaatgg atgccggtgg cgcgcgccat ttgcgcgatg
      481 cggctaatcg ttttttcgat ctcgacaggc gcgctcatca ttaaatccga tagttcatcg
      541 accagcacgg cgatgtaggg cagacgctcg ccgccctgct cggtcatcgt cgtattgtaa
      601 tcattcacat gccgcgcact gacctccacg aatttcttgt agcggccgtc catctcgcgc
      661 gcgatccagc gcagcacatt gacgatgcga tccatgtccg attcaacttt gccatacaaa
      721 tgcggcaggc cgttgaagcg gattaactct accatcttcg gatcgatcat caccagcttc
      781 aattcttcgg gcgtgttgtt catgatcaac gacatgatga ttgcgttaat gcacacgctc
      841 ttgcctgaac cggttgtgcc tgcgatcagc acgtgcggca tcgaagcgag atcggctgcg
      901 atcgcgccgc cgctgacatc acgccccaat gcaaacgcca gcggctcgcc gatctttttg
      961 aagtcctcgc tctccaatac accgcgcaag cccaccatgg caatctgatc attcggcact
     1021 tcaatgccga caaatgtttt gcctggcacg ggcgcctcga tccgcaacga ctgcgcggcc
     1081 agcgccagcg ccagatcatt cgacaatgaa ctgatctgac ccacgcgaac tttttgcttg
     1141 cgcgcaacgc catccggccc cggtcgatca atatagccgg gcgcaacctg aaattgtgta
     1201 acggccggac cctgcgctgt attcaccact tccgcttcaa gcccgaactg tttcaacgtc
     1261 tgctcgatca cctgcgactt gcgatcgatc tcttgctgct cgatcgtcga agccgtgtgc
     1321 tgctccaaga gatcaagcgc tggcatgatc cgctcgcgga tgagcttcgg cttcgccggt
     1381 tcaatctttg gctcgatcgt tctcgctagc ccaacaacgg tcttcatcac tgttaacggt
     1441 aactgcgtcg ccgtgatggg cgcttgatcg atccgcgtgg gtttgatcct caccgctttc
     1501 gcgggcacaa cgtcttcgcc ctgcaccgga ttgatcgacg ccgcgacagc cggagggcta
     1561 accgatactc catcagcgga cg
//
LOCUS       DASRTJ010000301         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93149,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000301 DASRTJ010000000
VERSION     DASRTJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93149"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFF70_06080"
     CDS             complement(<1..477)
                     /locus_tag="VFF70_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44303.1"
                     /translation="MLIRPLVSHDLPHLQNIDANFTSNRFLDIHKIVDGFNISWQLIE
                     KKLDPPFVSTDYNIPDTEIEELADRLRKNDGLYLAVEDDGKLIAFLDMPREHWRHTAL
                     ISNLYVDQKFRRQRVGAELINRALQYAQENSIRAIWLETQSNNYPACQFYRSRGFEV"
     gene            complement(691..2103)
                     /locus_tag="VFF70_06085"
     CDS             complement(691..2103)
                     /locus_tag="VFF70_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZY44304.1"
                     /translation="MLQYKRTANLADVRERIVGINQQVPLLNGETRKYVNFDNAASTP
                     VLRDVLDTVNDFMNWYSSIHRGTGFKSKVATQAYEDARHIVAEFVGANLTDHTVIFGK
                     NTSEAINKLSYRFPLAKEDVVLVSLLEHHSNDLPWRAKAHVEHIRADDLGHLDEADLD
                     RLLQQFKGRVKLVAITGASNVTGFMPDVHRIAEKVHVAGAQIVVDCAQLAPHRLIAMG
                     ALDDPAHFDYVAISAHKMYAPFGTGALIGRRDTFEVGEPEYRGGGTIEIVTVDDVEWS
                     GSPDRDEAGSPNVVGAVALAAAIKALNKIGMSSIADHEAELTAYALEHLAPIEGLQIY
                     GDTDPRSTAGRLGVIPFNLKDRSHFLVAAILGAEYGIGVRNGCFCAHPYLLHLMGLSQ
                     AESNRVRQEILANDRHEMPGMIRISFGMYNTAEEVEVAVQALANIARGKYYGRYEQDA
                     KSGEFNAVGWKPDLARYFKL"
     gene            complement(2112..3095)
                     /gene="era"
                     /locus_tag="VFF70_06090"
     CDS             complement(2112..3095)
                     /gene="era"
                     /locus_tag="VFF70_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZY44305.1"
                     /translation="MNESLQVHNTDLTATPPHFRSGYVAVIGKPNVGKSTLLNAYLGQ
                     KISIVSNKPQTTRDRIIGILTQPTAQVIFIDTPGIHLPKHALGQYMIDVAYGAIAEAD
                     TIVFMVDLSERPDDNDRRIAEALRDQPEIRSVILALNKMDHLLSPEEMKAHVETYWSL
                     APTPMGQEPPAPWDWIMLSATQGTNSVELLDKIIAALPEGPRYYPADQVTDQQERDIA
                     AELIREQALLHLKQEVPHSVAVAIEQYDERPNGSVHIAATIYVERDSQKGIMIGSGGK
                     MLKQIGADARHEIQRLVDNKVFLELWVKVRKNWRQDERELRDLGYTGKSNG"
     gene            complement(3159..>3655)
                     /locus_tag="VFF70_06095"
     CDS             complement(3159..>3655)
                     /locus_tag="VFF70_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZY44306.1"
                     /translation="HIPILSAPEDMFGKVMSVPDAAMPAFFKLVTRYMPNQVAAIEKG
                     LADGSTHPRDVKMELAREIVSIFHNPDSVAAAEQHFINVFQQREMPEQMDEIKIDHAL
                     TITDLLISTQLAKSKNEARRLIDQGGVKLDSTAIDDAMMLIEVKSPLVLQVGRRKFVR
                     LMPK"
BASE COUNT          770 a          989 c          995 g          901 t
ORIGIN      
        1 tacttcaaag ccgcgcgatc gataaaactg gcaggccgga taattgttcg attgtgtttc
       61 cagccaaatg gcgcgtattg agttttcttg agcgtactgc agagcccgat tgattaactc
      121 ggcccccacg cgctgtcggc ggaatttttg atcgacgtat agattcgaga tcaacgccgt
      181 atgtcgccaa tgctcgcgcg gcatgtctaa gaaagcgatt aacttgccgt cgtcttcgac
      241 cgccaaatac aacccatcat tttttcgcaa ccgatcggcc agttcttcaa tttcagtgtc
      301 gggaatattg taatcggtgc tgacgaacgg cggatcgagt ttcttttcga tcagttgcca
      361 cgaaatgttg aagccatcga caatcttatg aatgtcgaga aatcgattgg aggtgaaatt
      421 cgcatcgatg ttttggagat gaggtaagtc gtgtgaaacg agtggcctga tgagcatagc
      481 ataatcagtg aatagtcatt agtggaaatg cactactaat gcaagttacg caggctaagc
      541 gtcattgcga ggaggtcgtt aggctgacgt ggcaatctcc cgctcgatat tgagattgct
      601 tcgttcacct gcacttgcgc gctaccgcgc gcagcgcggt gcaagtgtcg ctcgcaatga
      661 cggtgagtaa caacaattac taattactat ttacagctta aaatatcgtg cgaggtccgg
      721 cttccagcca accgcattga actcgcccga cttcgcgtct tgctcgtaac gtccatagta
      781 tttgccgcgc gcgatattgg ctagcgcttg caccgccacc tcgacttctt cggccgtgtt
      841 gtacatgccg aaactgatac ggatcatgcc cggcatctcg tgtcgatcgt tggcgagaat
      901 ttcctgacgc acacgattgg attcggcctg cgacaggccc atcaaatgca gcaaataagg
      961 atgtgcgcag aaacagccgt tgcgcacgcc gataccgtat tcggctccca agatcgcggc
     1021 gactagaaaa tgcgacctgt ccttgaggtt gaacggaatc acgcctaatc gtccggcggt
     1081 cgatcgggga tcggtgtcgc cataaatttg cagaccctcg atcggcgcca gatgttccaa
     1141 cgcatacgcg gtaagttcag cttcatgatc ggcgatgctg ctcatgccga ttttgttcag
     1201 cgctttgatc gccgcagcta acgcgaccgc accgacgacg ttcggcgagc cagcttcatc
     1261 tcgatcgggt gaacctgacc attcgacatc gtcgacggtt acgatctcga tcgtgccgcc
     1321 gccgcgatat tcgggttcgc cgacttcgaa cgtatcgcgc cgcccgatca aggcgccggt
     1381 cccgaagggc gcgtacatct tgtgcgcgga aattgcgacg taatcaaagt gtgcgggatc
     1441 gtccagcgcg cccatcgcta tgagacgatg cggtgcgagc tgcgcgcaat cgaccacgat
     1501 ctgcgcgcca gccacatgaa ctttctcggc aatgcgatgc acgtccggca tgaagccggt
     1561 cacgttcgat gcgccggtga tcgccaccag tttgacgcgt cccttaaatt gctgcaacaa
     1621 ccgatcgaga tccgcttcgt ccaagtggcc gagatcatcg gccctgatat gttcgacgtg
     1681 tgctttggcg cgccacggca gatcgttgct gtgatgttcg agcagagaaa ctaaaaccac
     1741 atcctctttg gccagcggaa atcgatacga aagtttattg atcgcttcac tagtattttt
     1801 gccgaagata accgtgtgat ccgttaaatt tgcgccgacg aattccgcca cgatatggcg
     1861 cgcatcctca tacgcttgcg tcgccacttt gctcttgaag ccggtgccgc gatgaatcga
     1921 cgaataccaa ttcatgaaat cgttgacggt atccagcaca tcgcgcagca ccggcgtgct
     1981 ggcggcgttg tcaaagttga catacttgcg cgtttcgccg ttcagcagcg gcacttgttg
     2041 attgatgccg acgattcgct cgcggacatc cgccagattc gcggtgcgtt tatattgcag
     2101 catggttccc cctatccatt actcttaccc gtgtaaccca ggtcccgcag ctcgcgctcg
     2161 tcctgccgcc agttcttgcg gaccttcacc cacaattcca aaaacacttt gttatcgacc
     2221 aggcgttgaa tctcatgccg cgcatcggcg ccgatctgct tgagcatctt gccgccgctg
     2281 ccgatcatga tccctttttg tgaatcgcgc tcgacgtaga tcgtcgcggc gatgtggacc
     2341 gatccattgg ggcgttcatc gtattgctcg atcgctacgg cgaccgaatg cgggacctct
     2401 tgcttgagat gcagcagcgc ctgctctcga atcaactctg cggcgatgtc acgttcctgc
     2461 tgatcggtga cttgatccgc cggataatag cgcggacctt cgggcagggc ggcaatgatc
     2521 ttgtctagca actcaacgga gttggtgccc tgcgtggccg ataacatgat ccagtcccac
     2581 ggagcgggcg gttcctgccc catgggagtt ggggcaagtg accaatacgt ttcaacatgt
     2641 gctttcattt cttccggcga gagaagatga tccattttat tgagagccag aatcaccgat
     2701 cggatttcag gctgatcgcg cagcgcttcg gcaatccgcc gatcgttgtc atcgggccgt
     2761 tcgctcagat cgaccatgaa cacaatcgta tcggcctcag cgatcgcgcc gtaagccacg
     2821 tcgatcatgt actgaccgag cgcgtgtttg ggcagatgaa tgccgggcgt gtcgataaag
     2881 atcacctgcg cggtgggctg cgtgagaatg ccgatgatgc gatcgcgtgt ggtctgcggc
     2941 ttgttgctga cgatggaaat tttttggccc aggtatgcgt tgagcaaggt ggacttgccg
     3001 acattcggct tgccgatcac agcgacgtag ccggatcgaa agtgaggagg agtggcggtc
     3061 agatcagtgt tgtggacttg tagtgattcg ttcataagta ggagtaatta gtggttagta
     3121 attagtgaaa atccgatttg ctaattactc attgctaatc atttcggcat taaccttaca
     3181 aattttcgtc tgccaacctg caagaccagc ggcgacttca cctcgatcag catcatcgca
     3241 tcgtcgatcg ctgtgctgtc gagcttgacg ccgccttgat cgatcaagcg gcgcgcctcg
     3301 ttcttgctct tggctaactg cgttgatatt aggagatccg tgatcgtcag cgcgtgatcg
     3361 atcttgatct cgtccatctg ctctggcatc tcgcgctgtt gaaagacgtt gatgaaatgc
     3421 tgttcggcgg cggccacgct gtccgggttg tggaagatgc tgacgatctc gcgggcgagt
     3481 tccatcttca cgtcgcgtgg atgtgtcgat ccgtccgcta gccctttttc gatcgctgcg
     3541 acttgattgg gcatataacg cgtgacgagt ttgaagaaag cgggcatcgc cgcatccggc
     3601 acgctcatca ctttgccaaa catgtcttcg ggcgcggaca agattggaat atgat
//
LOCUS       DASRTJ010000302         7035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_272729,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000302 DASRTJ010000000
VERSION     DASRTJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272729"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1142)
                     /locus_tag="VFF70_06100"
     CDS             complement(306..1142)
                     /locus_tag="VFF70_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44307.1"
                     /translation="MDIQLADQRIFALSEKITPDQAKQRAMDKRVQIFISGLGSMILQ
                     RPKSEEIELIGTQKRFEPFWHVVCATRHVYDRSSKFNVPVAGAEVKSITLQGTDYSVT
                     NHAFTLSALEHCTEEHREQIFVNGVNGEKEELGGVISGPKNEVTDLSTFTTDDKAIVV
                     PPETRASFVVRQILQPMLKPVQADVILEETVTIEAVDLYYRPIYAFEFKWKPKDKTGV
                     AEFDGVTGEMRNAKALLQKMNIPLTRNALFDIGADTLGMLVPGANIALKLARVAMDKE
                     KY"
     gene            complement(1251..2123)
                     /locus_tag="VFF70_06105"
     CDS             complement(1251..2123)
                     /locus_tag="VFF70_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44308.1"
                     /translation="MDVHLGATHIYALNPALDFATAQQQATDKRLNVVAGGLGAVFSR
                     PKPEEVDLTYSETRYEAFWHLVCTVRYVFERKKDFNVAVAGNEVRKVTLLGQEFEVTA
                     PPAAQGGGGFLQQIGVGGAAHSFAIPGVEYCVDENKQERFLDAATGQVLQAAAEYVKK
                     DKSEIVDLTLLTSGENIVVPPQQSASKIAKALLATMAKQIQADKILEDTVEINTLDLY
                     FRPIYAFEFTWKTKSKTGIAEFDAATGNMIAGKVVRGKVEKPITTAELFDINPETVTS
                     LVPTTAGNVKLITA"
     gene            complement(2288..3538)
                     /locus_tag="VFF70_06110"
     CDS             complement(2288..3538)
                     /locus_tag="VFF70_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZY44309.1"
                     /translation="MTTPWAERYALRTHTMSSSAIRELLKLTQQPDIISFAGGLPAPE
                     LFPIEEVKAACDVVLSETGSQSLQYSTTEGYPPLRELLVRHMARYGIKVDIDNIVITS
                     GSQQALDLIGKLLINPGDRVLTENPTYLGALQAFSAYQAQYVTVPIDDDGLQVGRLEA
                     ALRVGPKFMYILPNFQNPAGVTLSATRRYDLIALAEQYGIPIIEDDPYGQLRYEGDHI
                     KPLVVLDSEYLECSDNGGYSGNVIYTSTFSKTLAPGLRLGWVVAPKDVVAKLVMMKQG
                     TDLHTSTFDQMVACEVAHGGFLDRHIKTIREVYHHRRDVMLNALEKKMPDGVKWTRPL
                     GGLFLWVTLPESIAAVDLLKDAIKEKVAFVPGDAFHPNGGGRNTMRLNFSNAGDEMIE
                     EGIRRMSLAIKQRLTQSSTMFSLS"
     gene            complement(3680..4234)
                     /locus_tag="VFF70_06115"
     CDS             complement(3680..4234)
                     /locus_tag="VFF70_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZY44310.1"
                     /translation="MQDDIIFSGFGGQGALFAGQLLAYAAMDAGKHVTWIPSYGPEMR
                     GGTAHCVVIISDEEIGSPLVRNPSNVIALNNPSVEKYEALLAADGNLIYNSSLTTHVI
                     TRRNIRALPIPASDIAAELGHAKLLNMIMLGALIDRTQVLPIEAIDQALNDHIPAHRR
                     ELLELNKLALRRGIESGHTILVPV"
     gene            complement(4293..5054)
                     /locus_tag="VFF70_06120"
     CDS             complement(4293..5054)
                     /locus_tag="VFF70_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZY44311.1"
                     /translation="MATQLDEQIVYQRPETLSNVATHYCPGCTHGIAHRLVAEVIDEL
                     GIREKTIGVAPVGCSVFCYNYFECDFAEAAHGRAPAMATGLKRVMPDKVVFTYQGDGD
                     LASIGLAEIMHAALRGENITVIFINNAIYGMTGGQMAPTTLPGQKTTSSPNGRDVEMS
                     GFPIKMSELIAQMQGATFVARRSLHNPTEIRKAKKSIKQAFENQLNGFGFSMVELLSS
                     CPTNWDINPADSLKFIETQMVPVYPLGDYKNVKNN"
     gene            complement(5058..6179)
                     /locus_tag="VFF70_06125"
     CDS             complement(5058..6179)
                     /locus_tag="VFF70_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HZY44312.1"
                     /translation="MTKKLLKGNEAIAEAAIRAGCEAYFGYPITPQTELLEYMAKRMP
                     ELNRAFVQAESEVAAINMVYGAACTGKRVMTSSSSPGVSLMQEGLSYIAGSEVPCVIV
                     DVMRGGPGLGNIAPSQGDYFQMVKMAGHGDYKPIVLAPATVQEAIDLTVLAFDLAETY
                     RTIVTVLLDGNIGQLMEPAELPPMQASNRQLPPWALTGAIGRKKNIISSIHLVPEEEE
                     LFNRKLQEKIAIIQRNEVRFKEYQTDGAELIVVGFGTSGRVALTAVKMARQQGIPVGL
                     LRPISLFPFPADRLSQLADDARGFLVVEMNAGQMIDDVKIAVGGQAPVEFYGRMGGMV
                     PLPDEILAAIAKLDQATLRSRPVSNARASHVQLEPMLGR"
     gene            complement(6226..6474)
                     /locus_tag="VFF70_06130"
     CDS             complement(6226..6474)
                     /locus_tag="VFF70_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY44313.1"
                     /translation="MAAKGRIEIDAEHCKGCELCTGVCPQNVIRMSDTFNAKGYHPAQ
                     LIDPNLDCTGCAVCAVICPDTVITVYRQVVPRAALAAA"
     gene            6581..6763
                     /locus_tag="VFF70_06135"
     CDS             6581..6763
                     /locus_tag="VFF70_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44314.1"
                     /translation="MSYLQTLMLIQSQDKCHTNKDDREQFWLILPVWQLREFQIKTAI
                     VSIAIQPTAIKANIAN"
     gene            complement(6827..>7035)
                     /locus_tag="VFF70_06140"
     CDS             complement(6827..>7035)
                     /locus_tag="VFF70_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="osmotically inducible protein OsmC"
                     /protein_id="HZY44315.1"
                     /translation="IKIIQLMQTDRTTGLVGKIDLDIQLPPAFPEKYKSAVIRSAEQC
                     AVKKHLENPPKFNVYTSAAEPVPA"
BASE COUNT         1527 a         1925 c         1855 g         1728 t
ORIGIN      
        1 ctgcgtagat cgaccacgtc tcgtcgaaac cgccaaagcc acccgatcga tgatagacga
       61 cgattggatc tgtgttgttg ggcggagtga gcgccggagc ggcagcacag ctaattaagg
      121 caatgactaa cagtccataa actaaattaa gaaacttttt catgatgagt ctcctccctt
      181 tgaatagacg aaacggccag cagaaagttc catctgttgg ccgctgaaaa attgtaggcg
      241 gatatacgcg gataccacag agtgtatttt tacttttatc cgtgaagtcc agttaatccg
      301 tgcccttagt atttctcttt gtccatcgcg actctagcta acttcaaggc gatgtttgcg
      361 ccgggcacca acatgcctaa tgtgtctgca ccgatatcga acaacgcatt gcgggtgagc
      421 gggatattca tcttttgcaa cagagccttg gcattgcgca tctcgccggt cacgccatcg
      481 aactcggcca cgccggtttt gtctttcggc ttccacttga attcgaatgc gtaaatcggc
      541 cgataataaa gatcgaccgc ttcgatcgtc accgtttctt caaggatcac atcggcttgc
      601 accggtttca acatcggctg cagaatttgc cgcacgacga atgaggcgcg cgtctctggc
      661 ggcacgacaa tcgctttatc atcggttgtg aaagtgctga gatcggtcac ttcattcttt
      721 ggcccgctga tcactccgcc gagttcttct ttctcgccgt tcacgccatt cacgaaaatc
      781 tgctcgcgat gttcttccgt gcaatgctcc agcgcgctca gggtgaacgc atgattggtc
      841 acggaataat ccgtgccttg cagtgtgatc gatttcactt ctgcgccggc aaccggcaca
      901 ttaaatttgc tcgatcgatc gtaaacatgc cgtgtggcac agaccacatg ccagaacggc
      961 tcgaagcgct tttgtgtgcc gatgagttcg atctcttccg acttggggcg ctgcaaaatc
     1021 atcgagccga ggccgctgat gaaaatttgg acgcgcttat ccattgcgcg ctgcttggct
     1081 tgatccggcg tgattttttc gctgagcgcg aagatgcgtt gatcggctaa ttgaatgtcc
     1141 atagttccct catcaagaat aacgtagccg aggcatccca acagctgcac tgcaacgcat
     1201 gcagtgcggt gcaagtgtgc ctcggtagaa acgtcatcgg cctgacgttg ctacgcagtg
     1261 atcaacttca cattgcctgc cgtcgtcggc acgagcgaag taactgtctc agggttgatg
     1321 tcgaacagtt ctgctgtggt gatcggtttc tcgactttgc cgcgcaccac tttgccggcg
     1381 atcatattgc cggtcgccgc atcgaattcg gcgatgccgg tcttgctctt ggttttccac
     1441 gtaaattcga aggcgtagat cggccggaag tacagatcga gcgtattgat ctcgacggtg
     1501 tcttccagaa tcttatcggc ttgaatttgt ttagccatcg tcgccaataa cgctttggcg
     1561 atcttgctgg ccgattgctg cggtggcacg acgatgtttt cgccggaggt gagcagcgtc
     1621 agatcgacga tttctgattt gtctttcttc acgtattcag ccgccgcttg aagaacttgg
     1681 cccgtcgccg catcgaggaa gcgctcttgt ttattttcat cgacgcaata ttcgacgccc
     1741 gggatcgcaa atgaatgcgc cgcgccgcca actccgatct gttgcaaaaa gcctccaccg
     1801 ccctgtgcgg cgggcggcgc agtgacttca aactcttggc cgagcaaagt tactttgcga
     1861 acctcgttgc ccgccaccgc gacgttgaaa tcttttttac gctcgaacac gtagcgaacg
     1921 gtgcagacta agtgccagaa ggcttcgtag cgtgtttccg agtaagtcag atcgacctcc
     1981 tccggcttgg gtcgcgagaa gactgcgccg agtccgcccg cgaccacatt caagcgtttg
     2041 tcggtggctt gctgttgtgc ggtcgcaaaa tcgagcgcgg ggttgagcgc ataaatgtga
     2101 gttgctccaa gatgtacgtc catgctaatg cctccatgaa gtttgtaaat gagtgagtag
     2161 gtataacgct tagagtatac accgagttcg ggcaaatata aaattctgag ggataacaaa
     2221 aatccgatcg ggctggtcac ccgatcggat tttcaggcga gccgcgactc gcctttacaa
     2281 tttgtatcta cgataatgag aacatcgtcg aagactgcgt tagtcgctgc ttgatggcca
     2341 gcgacatgcg gcggatgccc tcttcgatca tttcatcacc tgcgttacta aaattgaggc
     2401 gcatggtgtt gcgcccgcca ccgttcggat gaaaagcatc gcccggcacg aatgcgactt
     2461 tttctttgat ggcatccttc aatagatcga ccgctgcgat cgattccggc agcgtgaccc
     2521 acaagaataa gccgcctaac gggcgggtcc acttcacgcc atctggcatc tttttttcga
     2581 gtgcattcag catcacatcg cgtcgatgat gatacacctc gcggatcgtc ttgatgtgcc
     2641 gatcgagaaa gccgccgtgc gccacttcac aggccaccat ctgatcgaag gtgctggtgt
     2701 gcagatcagt gccttgcttc atcatcacca atttcgccac cacatctttc ggcgcgacga
     2761 cccagcccaa gcgcagaccc ggcgcgagtg ttttgctgaa ggtgctggtg tagatcacgt
     2821 tgccggagta gccaccattg tcgctgcatt ccaaatattc cgaatcgagt acgactagcg
     2881 gcttgatgtg atcgccctcg taacgcagtt ggccgtacgg atcatcttcg atgatcggga
     2941 tgccatactg ttctgccagt gcgatcagat catagcggcg cgtggccgat aaggtcacgc
     3001 cggccggatt ttgaaaattc ggcaggatat acatgaactt cggaccgacg cgcagggctg
     3061 cttccagccg gccgacctgc aggccgtcat cgtcgatcgg tacggtgaca tactgcgctt
     3121 ggtaggcgct gaaggcttgc agcgcgccga ggtaagtggg gttctcggtc aagactcgat
     3181 cgccgggatt gatcaataat ttgccgatca agtctaaggc ttgctgtgag ccggacgtga
     3241 tcacaatgtt atcaatatcc actttaatcc catagcgcgc catgtgccgc accagcaatt
     3301 cacgcagcgg cggataacct tcggtggtgc tgtattgcaa tgattgtgat ccagtttcgc
     3361 tcaacactac atcgcaggcc gctttcactt cttcgatcgg aaacaattca ggcgcgggca
     3421 atcctccggc gaaggaaatg atgtccggct gctgagtcaa tttgagcagt tcacgaattg
     3481 ccgaactgct catggtatga gtgcgcagtg cataacgttc tgcccaaggt gtggtcattt
     3541 ttcctcttgt tgatcgtcat tgcgacaagc attctgatcg gagtctcgaa ctcgcagaag
     3601 tcgttgggga gccgaagggc gcgacgaagt aatctcaaaa tcgatcggga gattgcttcg
     3661 cttcgctcgc aatgacgcgc tagactggaa ccaaaattgt gtgtccgctt tcaatccctc
     3721 gccgcaacgc gagtttgttc aattccagca actcgcgtcg atgggctggg atgtgatcgt
     3781 ttaacgcttg atcgatggcc tcgatcggca ggacctgcgt ccgatcgatc aacgcgccca
     3841 acatgatcat gttcaatagc ttcgcgtggc ctaactcagc ggcgatatcg ctggccggaa
     3901 tgggcagcgc ccgaatgttg cggcgggtaa tgacgtgcgt cgtaagtgac gagttataga
     3961 tcaagttgcc gtcggcggcg agcagagctt catacttctc cacggatggg ttgtttagcg
     4021 cgatgacgtt ggatggattg cgcaccagcg gtgagccgat ttcttcgtcc gaaataatca
     4081 cgacacagtg cgctgtgccg ccgcgcatct ccggcccata agatggaatc caagtcacat
     4141 gcttgccggc atccatcgct gcataggcta agagttgtcc tgcaaataat gcgccttgcc
     4201 cgccgaagcc gctgaagata atgtcgtcct gcatgagagt ctccatgctg agtgattagt
     4261 aaattgttaa gtgccccact aattactcag cactaattat tttttacgtt cttgtaatcg
     4321 cccaacggat ataccggcac catctgcgtc tcgatgaact tgagtgagtc ggcgggattg
     4381 atgtcccagt tggtcgggca acttgataat agttcgacca tcgagaagcc gaaaccgttg
     4441 agttgattct cgaacgcctg cttgatggac tttttcgctt tgcggatctc ggtcgggttg
     4501 tgcaggctgc gccgcgcgac gaaggtggcg ccctgcatct gcgcgatcag ttcgctcatc
     4561 ttgatcggga aaccgctcat ttccacatcg cgaccgtttg gcgatgaagt ggtcttctgg
     4621 ccgggcagcg tcgttggcgc catttggccg ccagtcatgc cgtagatcgc gttgttaatg
     4681 aagatcacgg tgatgttctc accgcgcagt gccgcgtgca tgatctccgc caacccgatc
     4741 gaggcgagat cgccgtcgcc ttgatacgtg aacacgactt tgtccggcat cacgcgcttg
     4801 agaccggtcg ccatcgcggg cgcgcggcca tgcgccgctt cggcaaagtc gcattcgaaa
     4861 tagttgtagc aaaacacgga gcagcccacc ggcgcaacac cgatcgtttt ttcgcggatg
     4921 cccagctcat cgatcacttc cgccaccagg cgatgcgcaa tgccgtgcgt gcagcccgga
     4981 cagtaatgag tggctacgtt gcttaaagtt tcaggtcgtt gataaactat ttgttcgtcc
     5041 aattgagttg ccatcgatta tctccctagc attggctcaa gctgcacgtg actggcccgc
     5101 gcatttgaaa ctgggcgtga tcgcagcgtc gcttgatcga gtttggcaat tgccgccaga
     5161 atttcatcgg gcagcggcac catgccgccc atgcgtccgt aaaattccac gggcgcctgt
     5221 ccacccaccg caatcttcac atcgtcgatc atttgtcctg cattcatttc aacgacgagg
     5281 aaaccgcgcg catcatccgc caattgcgac aggcggtcgg ccgggaaggg gaacaaactg
     5341 atcggccgta acagcccaac cggaatacct tgctgtcgcg ccatcttcac cgcggtcagc
     5401 gccacgcggc ccgatgtgcc gaagccgacg acaatcaact ccgcgccatc tgtctgatat
     5461 tctttgaaac ggacttcatt ccgctggatg atcgcaatct tctcttgtaa tttgcgattg
     5521 aataactctt cttcttcagg taccaaatga atagacgaaa taatattttt cttgcgcccg
     5581 atcgctccgg tcaatgccca gggcggcaac tgccgattgc tcgcttgcat cggcggcagc
     5641 tcagcgggtt ccatcaattg gccgatattg ccgtcgagca acacggtcac gatcgtgcga
     5701 taagtctcgg cgagatcgaa cgcgagcacc gtgagatcga tcgcttcctg caccgtcgcg
     5761 ggtgcgagca cgatcggttt atagtcgcca tgcccggcca tcttcaccat ttgaaaataa
     5821 tcgccctggc tgggcgcaat gttgcccaag cccggcccgc cgcgcatcac gtcgacaatc
     5881 acgcatggca cttccgatcc ggcgatgtag cttaagcctt cctgcatcag gctgacgccc
     5941 ggacttgatg atgaagtcat cacgcgcttg cccgtacagg ccgcgccgta gaccatattg
     6001 atcgcggcca cttcgctttc ggcttgcaca aacgcgcgat tgagttccgg catgcgcttg
     6061 gccatatatt ccaataattc ggtctggggt gtaatcggat aaccgaaata tgcttcacag
     6121 ccggcgcgga tggcggcttc ggcgatggct tcattaccct ttaataattt tttagtcatg
     6181 ggcttctccg acccgcacgg cgctacgcag tggggcaagt gtcgatcatg cggcggcaag
     6241 cgcagctcga ggtaccactt ggcgataaac cgtgatcacc gtatccgggc agatcacggc
     6301 gcacaccgcg cagccagtgc agtccaaatt tggatcgatc aattgtgcgg gatgataacc
     6361 tttggcgttg aaagtatccg acatgcgaat cacattctgc ggacacactc cagtacacag
     6421 ttcacagccc ttacaatgtt cggcatcgat ttcgatgcgg cccttggcag ccatagcgcc
     6481 tcctgtatat tggatattgg agggtgcaaa gtatgtacac ctccaacttc caatttctag
     6541 tctcagcata aagctaatgc tgcggcttga cgagtgcggg atgtcatatc tgcaaacgct
     6601 tatgctcata caatctcaag ataaatgtca tacaaataaa gatgatagag agcaattctg
     6661 gttgatcctg ccggtctggc agctgcgtga atttcaaata aaaacggcga tcgtttcgat
     6721 cgccatccag cccaccgcaa ttaaggctaa tatcgctaat taaaaagatg aactgttcgg
     6781 atcggtcaag aaccccttag gtcttgtcga attactcagc gtgtgcctaa gccggcactg
     6841 gttccgctgc cgaggtatac acgttgaatt ttggcggatt ctccagatgt ttcttcaccg
     6901 cgcactgttc agccgatcgg atgacggctg atttgtactt ctctgggaag gccggcggca
     6961 gttgaatatc cagatcgatc ttgccaacta agccagtcgt ccgatcggtt tgcatgagct
     7021 gaatgatctt aattc
//
LOCUS       DASRTJ010000303        14373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93202,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000303 DASRTJ010000000
VERSION     DASRTJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93202"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1491)
                     /locus_tag="VFF70_06145"
     CDS             complement(28..1491)
                     /locus_tag="VFF70_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HZY44316.1"
                     /translation="MKPVKSVVLMFAAVSIGILITIGLLINLQHTAQARSIDSSKPPI
                     IASTTSTTPVLYLIKDINPITGSKPHYFNAIGNIVFFQADDGSHGNELWRSDGTEAGT
                     TLVKDINPGITSSGAIYNGNWATLNGIGIFDTNDGTHGYELWRTDGTLTGTIMITDIN
                     PSGNSYPSFMTTLGNAVYFQANDGFQGYGLWRTDGTLTGTTVVTNIYPSAPDIHFASL
                     NGALYFNAPDALHGFEVWRSDGTPANTYMITDIYPGITNSNATAFTVFNNKLYFNADD
                     GVHGFELWRTDGTPTGTLMITDINSSGNSNPAYLTPFNNALHFRATDGAAHGSEFWRT
                     DGTPGGTRMITDINPGGGNGSPSYFIIMDNTLLFGATDGINSGLWRSDGTPTNTLFIT
                     NIYPSLREAVFNGHLYFNGYDSTNGEELWRSDGTPTGTIMISDINPGFAAGLSNNYLT
                     PAGNHLFFQANNGTLGFELWAVKAGSEIFLPVVLKDF"
     gene            complement(1661..2356)
                     /locus_tag="VFF70_06150"
     CDS             complement(1661..2356)
                     /locus_tag="VFF70_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZY44317.1"
                     /translation="MPQTPHLEKHFTASESIRDIVIGMSDGLTVPFALAAGLSGAVDT
                     TTIIVTAGLAEMAAGSIAMGLGGYLAAKSDAEHYASEQARENLEIREKVDAERSEVMH
                     IFEEYGLTTQEAGPVVTALSKRPAAYVDFMMRFELGLEKPDPKRALRSAVTIALSYVA
                     GGLIPLLPYFLSSSAKAALPLSVVFTLIALFLFGYVKGRFTGAKPIRSAIQTMLIGGI
                     AAGAAFTIARLIS"
     gene            complement(2512..4020)
                     /locus_tag="VFF70_06155"
     CDS             complement(2512..4020)
                     /locus_tag="VFF70_06155"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZY44318.1"
                     /translation="MPTTYLEQLKTRLGQIDDLNRANALLSWDQQTYMPPGGATARAE
                     QLATLQEISHGMFSSDETGRLLDAATEETAQADPDSDEARLIKVTRRDYHKARKVPAD
                     LVAAIARATSKALEGWTQARKESDWKKFEPHLANIIDLDRQLADHLGYTDRMYDALLD
                     QYEPDMKTAQVKATFDALKPELINLVKAISPKVKSTDDQVLHRNYNEDKQWQFGLEVI
                     KRFGLDLSRARQDKSVHPFTTSFSINDVRLTTRVSRDFISPALFGTLHETGHALYEQG
                     SHQVYDRTPLAGGTSLGMHESQSRMWENLVGRSRPFWKYFFPLLKNTFPDQLADQTPE
                     SFYRAASAVKPSFIRVEADEVTYGLHIMLRFEIENEMLERKIKLSDVPEAWNAKMMDF
                     MGIVPPTTADGALQDIHWSIGSIGYFPTYQLGNLISLQLWEKINQDIPDLMAQIEHGE
                     FAALLTWLRQNVHGYGRKFTSNELLTRITGHGLDAGPYVKYLKQKYGDIYGI"
     gene            complement(4160..5503)
                     /locus_tag="VFF70_06160"
     CDS             complement(4160..5503)
                     /locus_tag="VFF70_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HZY44319.1"
                     /translation="MIDSDSIHDLLVRGIAAAKSNQPQDKEEARYFLNWALRSDGISY
                     EQQAEAWLWLSQVEDAPAKKRDCLENVLAIDPGNPLARRGLLILAGQLKPEDVTNPIG
                     PDQPIETSSAPKTRRYVCPRCGGSLAFDPNRKQLTCAYCGNHLYEYDAIQQGALAPIE
                     EQNFIATLSTRKAHIWELASAHTLHCQSCGAHFTLPPTAISGACPFCASPQVIESAAD
                     DQLLQPEGVLPFQFDADAALKHAHHWLSAQRFRPDDLERRSAIQKPHGLYLPFWTFDL
                     GGQMNWHAEIAEQRGRYTEWVPRTDLYIVLHDDLLVPGSHSVPIDLIEPIADFNTTAL
                     QPYSADLLADWSAEIYQVSLADASLVARQRAVQFAKKQIEHVNLAGERMRNLQLNSTG
                     IVIESFKLVLLPVWIGSYQYQSRSYRIALNGQTAKGTGQIPRSGWQKTLSNIFKN"
     gene            complement(5507..6322)
                     /locus_tag="VFF70_06165"
     CDS             complement(5507..6322)
                     /locus_tag="VFF70_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZY44320.1"
                     /translation="MPSSKITRREPYHGTQAVARAISILKVFENSTATLTAAQIAAQL
                     KLNRSTVHRLLSILETEGFVARESSASDDARHSAYRLGPGLVSLGGIALRQINIRSIA
                     LSHLRSLAQLSGETVDLEILIGREVMIIEEVQGEHVLRVGVGDNIGGRYPAHTTSTGK
                     ILLAALNEDDLKEFLASKLNALTPNTLIDKQLLREQIELIRKQGWASAWEELEIGLMA
                     VGAPIRDRDGQVIAAVSVSGPTVRINKSHIRQLAPLVIDCAKSISRDLGFMRR"
     gene            complement(6428..7189)
                     /locus_tag="VFF70_06170"
     CDS             complement(6428..7189)
                     /locus_tag="VFF70_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZY44321.1"
                     /translation="MTPIVKVTHLDKHFGQLHVLKDVSLSVDPTEVVCIIGRSGSGKS
                     TLLRCINFLEQPTHGSIEVDGLSVDVQQARLTGEQRQVLHEIRLHTGMVFQEFNLFPH
                     LSVIENLIEAPVTVKGTPRDQAIALAEKYLEQVGLMFKRDEFPNRLSGGQKQRVAIAR
                     ALTMEPKVMLFDEPTSALDPELIGEVLEVMKQLARNGMTMLVITHEMDFAREVANRVL
                     YMHEGEFIEEGTPARMFSDPRDPRTRMFLDRLLRQ"
     gene            complement(7192..8037)
                     /locus_tag="VFF70_06175"
     CDS             complement(7192..8037)
                     /locus_tag="VFF70_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZY44322.1"
                     /translation="MTTTHMAESNAQAQRILKQQDRNQRNFNLKVAGWWVVFFLFLVF
                     LFSGVKIDIGPIHIKTITMDQDFINEWAAFISGGVPQTLLISFLSIIFASILALVTAL
                     ARLSRFSPVYAAATFYTSLIRGTPLYLQIFFFFLAWPQLGFEPLPGFVAGVVALSLNY
                     GAYMSEIFRAGISSVSKGQRESALALGMTNGQMMRRIVLPQALRFVVPPTGNEFIAML
                     KDSSLVSATGFVQEIMWRATKVGRANFHGLEALLIAALWYWAMTIIFTWLQSRLELRM
                     SRGER"
     gene            complement(8164..9063)
                     /locus_tag="VFF70_06180"
     CDS             complement(8164..9063)
                     /locus_tag="VFF70_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZY44323.1"
                     /translation="MSASRKRISIVVSLLVLTAMILSACQQATADDLLGQIKTRGTIR
                     ISTDPNYAPQSSLVAAGQRAANTKCAADQLTAGQLEGFDIDTAVEVAKRLGVEACFVT
                     PTWDTITAGSWGGQWDISVGSMTITKPRQQVVFFSPGYYFTPAQFAVAKDSGINTLAD
                     LNGKPVCVGSSTTYEDYLNGKLGIPDSDIKAPAPTGVQVVSLETDAECAQAIQSGRKE
                     FAAFLTSATVVDQSISKGIPVVKLGPPVFIENLAVAIDKKASKDTKSLLDAISNAITA
                     MHTDGSLKTSSVKWFGADLTTLP"
     gene            complement(9289..10074)
                     /locus_tag="VFF70_06185"
     CDS             complement(9289..10074)
                     /locus_tag="VFF70_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY44324.1"
                     /translation="MKYTRQGISLEYDDLGNGTPLLFIHGFPLDRSMWGPQVNALQKI
                     CRVIAPDLRGFGQSSDTDGQAITMETYAEDMKALLDSLNVKQVVVCGLSMGGYIALAF
                     VAKYTDRVKGLILSNTKAGADTDAGRQGRLDTSIKIEQTGSTDIVVDGMAPKMLGPDA
                     KSETATFVRSMMSRQRSTTVSSALRGMAARPDRTPLLASIKFPTLIITGSADVVIPPS
                     ESEAMHTAIPHSTLVNIPEAGHLANLDKPEAYNEALQSFLSAG"
     gene            10318..11403
                     /locus_tag="VFF70_06190"
     CDS             10318..11403
                     /locus_tag="VFF70_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZY44325.1"
                     /translation="MAFNPLNAIFGLFSLDLGIDLGTANTLVNVRGKGIVLNEPSWVA
                     IDKRTKRPLAIGAEAREMVGRTPGNIVAIRPMRDGVISDFEITEAMLDYFIKKAHTQM
                     FPLIEPRPRVVVGIPSGVTEVERRAVYDACMSAGAREAYPIEEPIAAAVGVGLPIMEA
                     RGSMVIDIGGGTTEVAVFSLGGVVLSRSIRVAGDEMDEDIMQYVRQKYNLLIGERMSE
                     RCKINIGSAYPLPEEKIMTLRGRSILTGLPEMIDVSSIEIREAIATSVGLIVDLVKSA
                     LDATPPEVVADLMEQGIALAGGGALLQGLAQRLSEEIKVRVWVAPDPLTAVALGCGKL
                     LENLDQLRNILQSVDRGRRRIGSPVGA"
     gene            11552..12415
                     /gene="mreC"
                     /locus_tag="VFF70_06195"
     CDS             11552..12415
                     /gene="mreC"
                     /locus_tag="VFF70_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZY44326.1"
                     /translation="MNRSRSRLPLLIFTLIAIGLLVLHEGGQLQPIEDAAQTVLGPIE
                     RAVSTLFNGIGDLFSAVRDLRDLRDRNAQLEKANQDLLTENARLKGLENENTALKALL
                     NFTQENPSYQNQTAAVIGRDPSPYVHFITINAGSHENLKPGMPVVTAGGTLIGRIDQV
                     NLRSSKVQLLNDPSSAVNVKLQTSGVTGLAIGASDGSLLMQYIPLDAALYQNDIALTS
                     GLGGNLPRGLVVGQVSSIEKQAADLFQSAKLTPAADYDRLDIVLVITNFIPIDDTAPT
                     PIPTIVPTPTP"
     gene            12468..13409
                     /locus_tag="VFF70_06200"
     CDS             12468..13409
                     /locus_tag="VFF70_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZY44327.1"
                     /translation="MTTPTIPQTNQRHASRLLIALVITLLFVVVEVWAAVQSNSLALL
                     TDAAHNFTDVLAIALSWYALRLAARAAHSGKTFGYHRAGILAAFINSTTLALISIGIF
                     VEAVQRINQPLQVDENTLIVVSIIAFLVNAGTALLVRQGSATDLNMRSAFVHLTGDAV
                     ATLGAVLAGIGIKLTHWQPLDPIASILIGLLILRGAWSILRETIEILLEGTPRDVDID
                     QLLSDVQQVAGVRGVHDLHVWSINHSMRALSAHVLTDDIPISSGAALQQQINSLIAQR
                     YGITHATLQLECIGCEPDLLYCDLTTNEELLEAKRDA"
     gene            13502..14002
                     /gene="mreD"
                     /locus_tag="VFF70_06205"
     CDS             13502..14002
                     /gene="mreD"
                     /locus_tag="VFF70_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZY44328.1"
                     /translation="MSLYFTIPFLALLAIFQATAAPQISIASGHPDLLLLCVMSWELV
                     QARGEGYSWALIGGIGLDLLGGGPFGASILGLLAVTLVADWLGGGLFRDRVALPLITA
                     LAGTFAFHGVYLIVLTLFGWKIDIFDAIVRVMLPSAVLNMILSPIAYRLMSALHRRVT
                     PSGLTW"
     gene            14111..>14373
                     /locus_tag="VFF70_06210"
     CDS             14111..>14373
                     /locus_tag="VFF70_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44329.1"
                     /translation="MSTTTRQRFSNPRIVVLLAVSLAIMALFVLRLWDLQIIRGADFR
                     ALADQNRFRLESVDAPRGIIYDRNGIPLVRNAPSFEIQIIPAYL"
BASE COUNT         3128 a         3771 c         4069 g         3405 t
ORIGIN      
        1 cgctgtttta ttttgattac tgatcgatta aaaatctttc aagactaccg gcaagaaaat
       61 ttccgagcca gccttcaccg cccacaattc aaagccgagt gtgccattat tcgcttggaa
      121 gaacaaatgg ttgccggctg gggttaaata gttgttcgaa agcccagctg cgaaacctgg
      181 attgatgtcg gagatcatga tagtgcccgt tggcgtgccg tctgatcgcc ataattcttc
      241 cccattcgtg ctgtcatagc cattaaagta tagatggcca ttaaacactg cttcgcggag
      301 acttggatag atgttcgtga taaacagcgt gttggtgggt gtaccgtcgc tgcgccacag
      361 gccggagttg atgccatccg ttgcgccgaa caacagggta ttatccatga taatgaaata
      421 acttggagac ccatttcctc caccgggatt gatgtcggtg atcattcttg tgccgccggg
      481 cgtgccgtca gtgcgccaga attcagagcc gtgcgccgca ccatcggtgg cacgaaagtg
      541 taacgcgttg ttaaaaggtg tgaggtaggc cggattggaa ttgccgctag aattgatgtc
      601 ggtgatcatc agcgtgccgg ttggcgtacc atccgtgcgc cacaactcga agccgtgaac
      661 tccatcgtcg gcattgaagt acagtttatt attgaagacg gtaaaggcag tggcgttaga
      721 attggtgatg cccggatata tatcggtaat catataagta ttggcaggtg tgccatcact
      781 gcgccacacc tcgaaaccgt gaagggcatc gggcgcgttg aaatacagtg cgccattcaa
      841 gctggcaaag tgaatatctg gcgcgctggg gtagatgttc gtcacgaccg ttgtgccggt
      901 gagtgtgccg tctgtgcgcc acaacccata accttggaac ccatcgttcg cttgaaagta
      961 aacggcattg ccgagggtgg tcatgaaaga gggatatgaa ttgccagaag gattgatgtc
     1021 ggtgatcatg atcgtgccgg taagcgtgcc atcggtgcgc cacaactcgt agccatgcgt
     1081 gccatcattg gtgtcgaaga taccgatgcc gttcaatgta gcccagttgc cattataaat
     1141 ggcacccgaa cttgtgatac cggggttgat gtccttgacg agggtggtgc cagcctcggt
     1201 gccatcactc cgccataatt catttccatg actgccatcg tccgcttgaa agaaaacaat
     1261 attgccgatg gcgttgaagt aatgcggctt ggaaccggtg atgggattaa tgtccttgat
     1321 caaatacaaa acgggcgtgg tcgaggtggt gctggcgatg atcggcggtt tggaactatc
     1381 gatcgatcgc gcctgggcgg tgtgctgcag gttgatcagt agtccgatcg tgatcaaaat
     1441 tccgatcgag accgctgcga acattaaaac cactgatttc actggtttca tgtttgcccc
     1501 ctccaattgg ttttgaaaca ggaaacaaac taacttggct caatgaatac attttaacac
     1561 ctctgtcaag ggggacgcac gcatcacgaa ttaaacctga cagcgggcga atcgatcagg
     1621 tcgctccgcc cgctgttgat tgatttctat ctgcaggttc ttagctaatc aatcgcgcga
     1681 tcgtgaacgc ggctcccgcc gcaatgccgc caatgagcat cgtctggatc gcagagcgga
     1741 tcggtttagc gccggtgaag cggcctttga cgtagccaaa gaggaacagg gcgatcaagg
     1801 tgaacacaac cgataagggc agggcggcct tcgcgctgct cgacaagaag tagggcagca
     1861 atggaatcaa tccacccgcg acgtatgaca gcgcgatcgt cactgcgctg cgcagtgcgc
     1921 gtttcggatc cggcttctcc agacccaact cgaagcgcat catgaaatcg acgtaagctg
     1981 cggggcgttt actcaaggca gtgacgacgg gtccagcttc ttgcgtggtg agtccgtact
     2041 cttcaaagat gtgcatgacc tctgatcgct cagcgtccac tttctcgcga atttccaaat
     2101 tctcgcgcgc ctgctcactg gcgtaatgtt ccgcatcgct ctttgcagcg aggtaaccac
     2161 ccaagcccat ggcgatcgat ccagcggcca tttcggctaa gcctgctgtg acgatgatgg
     2221 tcgtcgtatc gaccgcgccg gataatccgg cggcgagcgc gaaaggcacg gtcagtccat
     2281 cggacatgcc gatgacgatg tcgcggatcg actcgctagc ggtgaaatgt ttttcgaggt
     2341 gaggggtttg cggcatgatc gatctcctaa cattaaaaac tgggatgctc accgtgccgt
     2401 ccccggcgcg gttggcgccg ccgcggacgg cggcttgagc gacaaattca ttttcacgaa
     2461 cttagtctaa ataaaaaacc ggcgaggtat cggccccccg ccagttttgt gttagatacc
     2521 gtagatgtcg ccgtattttt gtttcaggta tttcacgtac ggccccgcat ctaagccgtg
     2581 accggtaatg cgcgtcaaca attcgttcga ggtgaacttg cgcccatacc catgtacatt
     2641 ctggcgcagc cacgtcaaca acgcggcgaa ttctccgtgt tcgatctgcg ccattaagtc
     2701 tggaatgtct tgattgatct tctcccacag ttgcagcgag atcaagttgc cgagttgata
     2761 ggtggggaag taaccgatcg atccaatcga ccagtgaatg tcctgcagcg cgccgtcggc
     2821 cgtggttggc gggacgatgc ccatgaagtc catcatcttg gcgttccacg cttcgggtac
     2881 atcgctgagt ttgatcttgc gctccaacat ctcgttttca atttcaaaac gcagcataat
     2941 gtgcaggcca tacgtcactt cgtcagcttc gacgcgaatg aaggatggct tcaccgcgct
     3001 ggctgcgcgg taaaacgatt ccggcgtttg atcggctaat tgatcgggaa aggtgttctt
     3061 cagcagcgga aagaaatatt tccagaacgg gcggctgcgc ccgaccaaat tttcccacat
     3121 gcgcgattgg gattcgtgca tgcccagcga agtgccgccc gccaacggtg ttcgatcata
     3181 gacttgatga ctgccctgct cgtataaggc atggccggtt tcgtgcagtg tgccgaacaa
     3241 agccggcgag atgaaatcgc ggctgacccg cgtggtcaag cggacatcgt tgatcgaaaa
     3301 tgatgtcgtg aagggatgca ccgatttgtc ctgcctcgcc cgactgaggt caagcccgaa
     3361 ccgcttaatc acttccaggc cgaactgcca ttgtttatct tcgttgtagt tccgatgcag
     3421 cacctgatcg tccgtgctct tgacctttgg tgagatcgcc ttgaccagat taattaattc
     3481 cggtttgagc gcgtcgaaag tcgctttcac ctgcgcggtt ttcatatctg gttcgtattg
     3541 atcgagcagc gcgtcgtaca ttcgatcggt gtaaccaagg tggtcggcca gctgtcgatc
     3601 taggtcgatg atgttcgcca aatgcggctc gaatttcttc caatctgatt ccttgcgggc
     3661 ttgcgtccag ccttccaacg ctttggaagt tgcccgcgcg atcgcggcca ccagatccgc
     3721 tggaactttg cgggctttgt gataatcgcg ccgcgtgacc ttgatcaaac gtgcttcgtc
     3781 cgaatcggga tcggcctgcg ccgtctcttc ggttgccgca tctaatagac gtccagtttc
     3841 atccgaggaa aacattccgt gcgagatttc ttgcagcgtg gcaagctgct cggcgcgcgc
     3901 cgtcgcaccg ccgggcggca tgtaagtctg ctgatcccag ctcagcagcg cgttcgcgcg
     3961 attgagatcg tcgatctgac ccaggcgggt tttgagttgc tccaaataag tcgttggcat
     4021 aggcatcctt cgtagcggaa taagagtgag aggtttattg aggtaatctt agtcgctggt
     4081 tattatgacc aagtttatcg tagaaaactg cgaacgtcaa tcgaattcga tgacgatcag
     4141 caaatcggtg cgcggcgaac taattcttga aaatattgga cagcgtcttt tgccagccgc
     4201 tgcgcggaat ctgtccggtt cctttagcgg tttgtccgtt gagtgcgatg cgatacgacc
     4261 ggctttgata ttggtagctg ccgatccaga ccggcagcag caccagctta aaggattcga
     4321 tcacgatgcc ggtgctgttc aattgcagat tgcgcatgcg ctcaccggcc aagttcacat
     4381 gctcgatctg tttttttgcg aactgtactg cacgctgacg ggcaacgagc gacgcatcgg
     4441 ccagggacac ttgatagatt tcagctgacc aatcggctaa gagatcggcg gaataaggct
     4501 gcaaggctgt ggtgttgaag tcggcgatcg gttcgatgag atcgatcggc acggagtgac
     4561 tgcccggcac caagagatca tcgtgcaaga cgatgtacag atcagtgcgc ggcacccatt
     4621 cagtgtaacg accgcgctgc tccgcaatct ccgcgtgcca attcatttgt ccgcccagat
     4681 cgaatgtcca aaacggcaaa tataacccgt gcggtttttg gatcgccgat cgacgctcca
     4741 aatcatccgg gcgaaagcgc tgcgcgctca accaatgatg cgcatgtttc aaagctgcat
     4801 cggcatcgaa ttggaatggc agcacacctt ccggttgcag caattgatca tccgccgcgg
     4861 actcgatcac ttgtggtgaa gcgcaaaatg gacacgcgcc gctgatcgcg gttggcggca
     4921 gagtgaagtg cgcgccgcaa ctttggcaat gcagtgtatg cgcgctagcc agttcccaaa
     4981 tgtgcgcttt gcgcgtcgac agcgtcgcga taaaattttg ttcttcgatc ggcgcgagcg
     5041 cgccttgttg aatggcatca tactcatata aatgattgcc gcagtaagcg cacgttaatt
     5101 gttttcgatt tggatcgaag gccaggctgc cgccgcagcg cgggcagaca tagcggcggg
     5161 ttttgggtgc ggagctggtc tcgatcggtt gatctggccc gatcgggttt gtcacatctt
     5221 ctggttttag ttgaccggcc agaattaata ggccgcgccg agccaggggg ttgcccggat
     5281 cgatcgccaa tacattttcc aggcagtcgc gtttttttgc gggagcatct tccacttggc
     5341 tgagccacaa ccacgcctcg gcttgctgtt cgtaagaaat gccatcgctg cgcagcgccc
     5401 aattcaaaaa gtagcgtgct tcctctttgt cttgcggctg atttgattta gccgcggcaa
     5461 tgccgcgcac cagcagatcg tgaatgctgt cagagtcgat catcgatcag cgcctcatga
     5521 aacctaagtc acgcgaaatg cttttggcac agtcgatcac caacggcgcc agttggcgga
     5581 tgtgcgactt gttgatccgc accgtcgggc cggagacact gaccgccgcg atcacttgcc
     5641 catcccggtc gcgaatcggc gccccaacgg ccatcaagcc gatctccaac tcttcccagg
     5701 ccgaggccca accttgcttg cgaatcagct cgatctgttc gcgtaatagc tgcttgtcga
     5761 taagcgtgtt gggtgttaag gcgttgagtt tactcgccaa aaattctttg aggtcatcct
     5821 cgttcaaagc ggccaacaag attttgccag tcgaggtcgt gtgcgccgga taacgcccgc
     5881 cgatattatc gccgacgccc acgcgcagga cgtgctcacc ttgcacttct tcgatgatca
     5941 tcacttcgcg tccgattaag atttccagat cgaccgtctc gccgctgagc tgcgccagcg
     6001 atcgaaggtg ggacagggcg atcgatcgga tgttgatttg tctcaaagcg atgccaccga
     6061 gcgataccag cccggggcct aagcgatagg cgctgtggcg cgcatcatcg ctcgcagacg
     6121 attcacgggc aacaaagcct tcggtctcaa gaatactgag caagcgatga acggtgctgc
     6181 gattgagttt gagctgcgcc gcgatttggg cggcagttaa ggtagcagtg gagttttcaa
     6241 aaactttcaa gatgctgatc gcgcgcgcga cggcttgtgt gccatgatac ggttcgcggc
     6301 gcgtgatctt ggatgagggc atagtcatca gcgtcattgc gagcctattc ggagcgaagc
     6361 aatctcctag actatcaagt gagattgctt cgtcggccgc atggcctcct cgcaaggaca
     6421 gttggattta ttgtctcaac aaccgatcga ggaacatacg cgtgcgcgga tcgcgcggat
     6481 cggagaacat gcgcgccggt gtgccttctt cgatgaactc accttcgtgc atatacagca
     6541 cccggttggc gacctcgcgg gcaaagtcca tctcgtgtgt gatgacgagc atcgtcatgc
     6601 cgttgcgcgc cagctgcttc atgacttcca gcacttcgcc aatcaattcc ggatcgagcg
     6661 ccgaagtggg ttcgtcgaac agcatgacct ttggctccat cgtcaaggcg cgggcgatgg
     6721 ccacgcgctg cttctgcccg cccgataagc gattcgggaa ttcgtcgcgc ttgaacatca
     6781 agcccacttg ttccaaatat ttctcggcca gagcaatcgc ttgatcgcgc ggcgtgcctt
     6841 tgacggtgac cggcgcttcg atcaagtttt cgatcacgct caagtgcggg aagagattga
     6901 actcttgaaa gaccatgccg gtatgcagcc ggatctcgtg cagcacttgc cgctgctcac
     6961 ctgtcaaacg ggcttgttgc acatcgacgc tcagaccatc cacttcgatc gagccatgcg
     7021 tgggttgttc aaggaaattg atgcagcgca gcagcgtcga tttgccggaa cccgatcgac
     7081 cgataatgca cacgacttca gtcggatcaa ccgagagcga gacgtctttg agcacgtgca
     7141 gttggccgaa gtgtttatcg agatgggtga ctttgacgat gggggtcatg gttagcgctc
     7201 tcccctcgac atgcgtagct ctaacctcga ttgcagccac gtgaagatga tcgtcatcgc
     7261 ccaataccac agtgcggcga tgagcagcgc ttccaaaccg tggaagttgg cgcggcccac
     7321 tttagtggcg cgccacatga tctcctgcac gaagcccgtc gccgacacga gcgatgaatc
     7381 tttgagcatg gcaatgaatt cattgccggt cggcggtaca acaaagcgca gcgcttgcgg
     7441 caggacaatg cggcgcatca tctgcccatt cgtcatgccc aaggccagtg ccgattcacg
     7501 ctgacctttg ctaacacttg atattccagc gcggaaaatc tcactcatgt acgcgccata
     7561 gttcaaggac aaagcaacca cacctgctac gaagccgggc agcggctcaa aacctaactg
     7621 cggccaggct agaaagaaga agaagatttg caaataaagt ggtgtgccgc ggatcagcga
     7681 agtatagaaa gttgctgcgg catataccgg cgagaagcgc gacaagcgcg ctaaggcggt
     7741 gaccagtgcc aagatgctgg caaagataat ggacaagaag gagatgagaa gcgtttgcgg
     7801 gacgccgccg ctaatgaagg ccgcccactc gttgatgaaa tcctgatcca tcgtgatcgt
     7861 cttaatgtgg atcgggccga tgtcgatctt gacaccgctg aacagaaaga cgaggaacag
     7921 gaagaagacc acccaccagc cggcaacttt taaattgaag ttgcgttggt tgcgatcctg
     7981 ctgtttcaga atgcgctggg cttgggcgtt gctctcggcc atgtgcgttg tggtcatcgc
     8041 gtggtctccg ggccgcaggc tgcggtcggg ctgatcggat cgatcgagaa aacggtaaaa
     8101 ccaaaaataa atactaatca ggctgcggtg ctcataatcg ccgcagcctg atcgaacact
     8161 tgtttagggc agggtggtca gatcagcccc aaaccatttc acggatgacg ttttgagcga
     8221 gccatcggta tgcatcgcgg tgatggcgtt gctaatcgca tccagcagac tcttggtatc
     8281 cttagacgcc ttcttatcga tggcgaccgc caggttctcg atgaataccg gtgggcccag
     8341 cttcacgacc ggaataccct tcgagatgga ctgatcgacg accgtggccg aggtcaagaa
     8401 ggcggcgaat tctttgcggc cagattgaat ggcttgcgca cattcagcat cagtctccag
     8461 tgagacgact tgcacgccgg tgggcgccgg cgctttgata tcggagtcgg gaatgccgag
     8521 cttgccattc agataatctt catacgttgt agatgaacca acgcataccg gcttgccatt
     8581 taaatcagcc aaggtgttaa tgccggaatc tttagcgacc gcaaactgcg cgggcgtaaa
     8641 gtaataaccg ggcgagaaga acactacttg ctggcggggt ttagtaatcg tcatcgagcc
     8701 gacgctgatg tcccactgtc cgccccagct gcccgcggtg atggtgtccc atgtcggtgt
     8761 gacgaagcac gcttcaacgc cgaggcgctt cgcaacttcg acggcggtgt cgatgtcgaa
     8821 gccttccagt tggccggcag tcaattgatc ggcggcgcat ttggtgttag cggcgcgctg
     8881 accagcggcg accaatgaag attgcggcgc atagttcgga tcggtcgaga tgcggatggt
     8941 gccgcgcgtt ttgatctggc caagcagatc gtcggcggtg gcttgctggc aggccgataa
     9001 gatcatggct gtcaaaacga gcagactcac tacaatcgaa atacgtttgc gtgaagcaga
     9061 cattattctc ctcctgaagc gaatggatag ttcaagcaaa aatggttagt ggtcatgttg
     9121 gcgcaacgaa attagtcttc gaatgaatgt tgacatcacc tcttttttga tcagaggtca
     9181 ggtgttgcgc tgaaactggg ctgtctcaca atctgaacaa ccgcattgta gcacaaagcc
     9241 agcgatgtgt taaatatgca actcattcac gcgacgcact agatcgatct atccagcgct
     9301 taagaaagat tgcagtgctt cgttatacgc ttcaggtttg tcaagatttg ccaggtgtcc
     9361 agcctcggga atgttgacca gtgtgctgtg cgggatggct gtatgcatgg cttcgctctc
     9421 gctgggcggg ataaccacat ccgccgaacc ggtgatgatc aacgttggaa atttgatgct
     9481 cgcaaggagt ggagttcgat cgggccgagc ggccatgccg cgcaatgccg atgaaactgt
     9541 ggtcgatcgc tggcgtgaca tcatgctgcg cacgaacgtc gctgtttccg atttggcatc
     9601 cggccccaac atcttcggcg ccatgccatc gacgactatg tcggttgatc ctgtttgctc
     9661 gatcttgatc gaagtgtcca ggcggccttg tcgcccggcg tccgtatctg cgcccgcttt
     9721 ggtattcgat aagatcaaac ccttaacccg atcggtatac ttggcgacga aggccaaggc
     9781 aatgtaaccg cccatcgata agccgcacac cacaacttgc ttgacgttga gtgaatcgag
     9841 cagcgctttc atatcttcgg cgtatgtttc catcgtgatg gcttgaccat ccgtatcgct
     9901 cgattggccg aagccgcgca gatcgggtgc aatgacgcgg cagatttttt gcagagcgtt
     9961 gacttgcggt ccccacatcg atcgatcgag gggaaagccg tgaatgaaca acagcggtgt
    10021 gccgttgccg agatcatcgt attccagact gataccttga cgtgtatatt tcattgttga
    10081 ctcctcaatg tgggatgaac acgattatac cattcgagga aaggatgagg gctgaaagcg
    10141 gaaggctgaa tgaatctgac tgggatgatg gccgatctgg atatcagctt ctcgcctcga
    10201 acgccacaat ctcagtaatg gatcgactgt ggactattgt cctcggtctg tggtcgatta
    10261 gtgtatggta taattcgcgc ttgaacttgg cagctatgat tcaattgagc gaacgctatg
    10321 gcttttaatc ctctgaatgc aattttcggc ctattttccc ttgatctggg catcgatctg
    10381 ggcacggcca atacgctggt caacgtgcgt ggcaaaggca ttgtgctgaa cgaaccgtcc
    10441 tgggtggcga tcgataagcg cacgaaacgg cccttggcga tcggtgcgga agcgcgtgag
    10501 atggtcggtc gaacgccggg aaacatcgtg gcgattcgtc cgatgcgcga cggagtcatc
    10561 agcgatttcg aaattaccga ggcgatgctg gattacttca ttaagaaggc gcacactcaa
    10621 atgttcccgc tgatcgaacc gcgcccgcgc gtggtcgtgg gtattcccag cggcgtgact
    10681 gaagtcgagc ggcgcgcagt gtatgatgcg tgcatgtccg cgggtgcgcg tgaagcgtat
    10741 ccgattgaag agccgatcgc tgcggcagtg ggtgtcggtc tgccgatcat ggaagcgcgc
    10801 ggcagcatgg tgatcgacat cggcggcggc acgaccgaag tggcggtgtt ctcgctcggc
    10861 ggtgtggtgc tttcccgatc gatccgcgtg gccggcgacg aaatggatga agacatcatg
    10921 cagtacgtcc gccaaaaata taatctgctc attggtgagc gcatgtcgga gcgttgtaag
    10981 atcaatatcg gttcagccta tccgctgccg gaagaaaaga tcatgacact gcgcggccgc
    11041 agtatcttga caggcttgcc cgaaatgatt gatgtgtcga gcatcgaaat ccgcgaagcg
    11101 attgccacat ccgtcgggtt gattgttgat ctggtcaagt cggcgctgga cgctactccg
    11161 ccggaagtag tggccgactt gatggaacaa ggtatcgcgt tggccggtgg tggcgcactg
    11221 ttgcaagggc tggcgcagcg cttgagtgaa gagatcaagg tgcgcgtatg ggttgcgccc
    11281 gatccgctga ctgcggtggc attaggctgt ggcaaactgc tggagaatct cgatcagctg
    11341 cgcaatattt tgcagagtgt cgatcgcggt cgacggcgga ttggctctcc cgtcggcgct
    11401 tgatcattcg cgttacatga aagcttgaaa ttaaaaagtt ggaggttagg tgttaagggt
    11461 tggatgtctc gctccaactt tcaacgtcta gcctccaatt gctaatggcg cattccaacg
    11521 ccaaactttc aacttccaat ttcaaataga catgaatcga tcacgatctc gactgccatt
    11581 attgatcttc acgctgatcg cgatcggcct gctggtgctg cacgaaggcg gacagctgca
    11641 gccgatcgaa gatgctgccc agacggtgct cggtccgatt gagcgcgcgg tgtccacttt
    11701 gttcaatggt atcggcgatc tgttcagcgc ggtgcgcgat ctacgcgacc tgcgcgatcg
    11761 gaatgcccaa cttgaaaaag cgaatcaaga tctgctgact gaaaacgcgc ggctcaaggg
    11821 gcttgaaaac gagaacacgg cgctaaaggc gctgctcaac ttcacacaag aaaatccgtc
    11881 ctatcaaaat caaacggcgg ctgttattgg ccgcgatcct agtccgtacg tccacttcat
    11941 cacgatcaac gccggttcgc atgaaaacct gaaacccggt atgccggtgg tgacggccgg
    12001 cggtacattg attggccgga tcgatcaagt aaatttgcga tcgtccaaag tgcagctgct
    12061 caacgatccg tcgagcgcgg tgaatgtgaa actgcaaaca tcgggcgtga cgggcttggc
    12121 aatcggcgcg tcggacggct cgctgctcat gcaatatatt ccgcttgatg cggcactgta
    12181 tcagaatgat attgccttaa ccagcgggct gggcggcaac ttgccacgtg gattggtcgt
    12241 cgggcaggtg agttcgatcg agaagcaggc ggccgatttg ttccaatcgg ccaagttgac
    12301 gccggccgca gattacgatc ggctcgatat agtgctggtg attacaaatt ttatcccgat
    12361 cgatgacacg gccccaacgc cgatacccac gatcgtacca acgcctacgc cctaagcagg
    12421 gcaaggcatt ctcaatgcaa acttgagaga tgtgatctaa tatgaccatg accacaccaa
    12481 caatacctca aaccaaccaa cgtcatgcct cacgcttgtt gatcgccctg gtcatcacgc
    12541 tgcttttcgt cgtggtcgaa gtctgggctg ccgtacaatc gaacagcctg gcgttgctca
    12601 ccgatgcggc gcataacttc accgatgtgt tagccattgc gctgagctgg tatgcgttac
    12661 gcttggcagc acgcgcggcg cattcgggca agacgtttgg ttatcatcgc gccggcattt
    12721 tggcggcttt catcaactcc accacactcg cgctgatctc gatcgggatt tttgtggagg
    12781 ccgttcaacg tatcaatcag cccctgcagg tggatgagaa tactttgatt gtcgtatcca
    12841 tcatcgcttt tttagttaat gccggtacgg ccttattggt ccggcagggc agcgcgaccg
    12901 atttaaatat gcgcagcgcg ttcgtgcatt tgaccggcga tgcggtggcg acgttgggcg
    12961 ctgtgctggc cggcattggc atcaaactaa cgcattggca gccccttgat ccgatcgcga
    13021 gtatcctgat cgggctgttg attctacgcg gcgcgtggag tattctgcgc gagacgatcg
    13081 aaattctgct ggaaggcacg ccgcgcgacg ttgatatcga tcaactgctg agcgatgtgc
    13141 agcaagtggc cggtgtgcgg ggtgtgcatg atctgcacgt gtggagtatc aatcacagta
    13201 tgcgtgctct ctccgcgcat gtcttgacgg atgacattcc tatcagttcc ggcgcggcgc
    13261 ttcagcagca gatcaattcg ctgatcgccc aacggtatgg catcacccat gcgacgttgc
    13321 aactcgaatg cattggctgc gagcctgatc tgttgtattg cgatttgacg acgaacgaag
    13381 agctgcttga agcgaaacgt gatgcgtgat gcgtatctcg catcatcacg caataaacca
    13441 tctgcgatat gcgatgtgcg ataagcctgc ccagatcaaa attcaaagat cgtagatttc
    13501 cttgagcctc tacttcacga taccctttct ggccttgtta gcaatctttc aagctacggc
    13561 cgcgccgcaa atctctatcg cgtctgggca tcctgatcta ttgctgctat gtgtcatgtc
    13621 gtgggaattg gtgcaggcgc gcggcgaagg ttactcttgg gcattgatcg gcggcatcgg
    13681 acttgatctc ttaggcggcg gcccgtttgg tgcttcgatc ctcggcttat tggccgtgac
    13741 gctggtggct gactggctgg ggggtggcct gttccgcgat cgagtagctt tacccctgat
    13801 tacagcctta gcgggcacat tcgcgtttca tggcgtatac ttaattgtgc tgaccctgtt
    13861 tggctggaag atcgatattt ttgacgcgat cgtacgggta atgctgcctt ccgcggtgct
    13921 caatatgatc ctcagcccga tcgcttatcg attgatgtcg gcgctgcatc gacgagttac
    13981 cccatcgggc ttaacgtggt agcgagttat atccgtgacg accctaagat attttaacag
    14041 tctcagcatg ctttgggttt gcccgtgacc gcgaagcggt atgccgctgg cgttggcggt
    14101 taaacatttt atgagtacaa ccactcgaca acgtttttct aatccgcgca ttgtagtgtt
    14161 gctcgccgtt tcactggcaa ttatggcgct gttcgtgctg cgcctgtggg acttgcagat
    14221 cattcggggt gcagacttcc gtgcgctggc cgatcaaaac cgcttccgct tggaatctgt
    14281 cgatgcgccg cgcggcatta tctacgatcg caatggcatc ccgctggtgc gcaacgcgcc
    14341 cagttttgaa atccaaatca ttcctgccta tct
//
LOCUS       DASRTJ010000304         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_245023,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000304 DASRTJ010000000
VERSION     DASRTJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245023"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /gene="leuS"
                     /locus_tag="VFF70_06215"
     CDS             complement(<1..>1541)
                     /gene="leuS"
                     /locus_tag="VFF70_06215"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZY44330.1"
                     /translation="YTYSGADTFGRYMRMIGNDVFQPMGFDAFGIHGENYALKLGENP
                     AIVMPRNMTRFRETQIKRMGAAFDWTREVDTTNPRYYKWTQWIFIQLFKAGLAYRATS
                     LVNWCPKDLTVLADEQVIDGQCERCGTEVIKRELEQWFFRITKYAERLLDHSKADFTE
                     TTKTLQRNWIGKSTGAQVTFSIDGLGNEDARLEIFTTRPDTLWGATFMVLAPEHPLVD
                     RITTPDRATKVKAYVSATAKESEFDRADTARAKTGVFIGAYAINPVNQERIPIWIADY
                     VLMTYGTGAIMAVPAHDQRDFEFARKFDLPIKVVVQPIGKVLDPATMIEAWPHDGVMI
                     NSGPLDGVPADRSVEKTIEWLEQNGLGERSINYRLRDWLISRQRYWGPPIPMIFCDEH
                     GWQPVPDDQLPVLLPMTKNFRPTGTGKSPLASMPKFVKTTCPVCGKPARRETDVSDNF
                     LDSAWYFLRYISTEFDDRAWDKERVKNWLPVTHYMGGIEHSTLHHLYARFIWKAMFDL
                     GHIPH"
BASE COUNT          319 a          435 c          428 g          359 t
ORIGIN      
        1 gtgtgggatg tggccgagat cgaacatcgc tttccagatg aagcgcgcat acaaatgatg
       61 cagcgtcgaa tgttcgatgc cgcccatgta gtgcgtgaca ggcagccaat tcttcacacg
      121 ctctttatcc cacgctcgat cgtcgaactc ggtcgagatg tagcgcagga aataccacgc
      181 cgaatcgagg aagttatcgc tgacatccgt ttcgcgccgc gcgggtttgc cgcacactgg
      241 acatgttgtc ttcacaaatt tcggcatcga agcgagtggc gatttgcccg tgcccgtcgg
      301 ccggaaattt tttgtcatcg gcaacagcac gggcagttga tcgtccggta caggttgcca
      361 gccgtgttcg tcgcagaaga tcatcgggat gggcgggccc caataacgct gccgcgagat
      421 caaccaatcg cgcaagcgat aattgatcga tcgctcgccc aagccgtttt gttccagcca
      481 ctcgatcgtt ttctccaccg atcgatcggc gggcacccca tcgagcgggc cggagttgat
      541 catcacgccg tcgtgcggcc aggcttcgat catcgtcgcg ggatcaagta ctttaccaat
      601 cggttgcacg acaaccttga tcggcagatc gaacttgcgc gcgaattcga aatcgcgttg
      661 atcgtgcgcg ggcacggcca tgatcgcgcc agtgccgtac gtcatcaaca catagtcggc
      721 aatccagatc ggaatgcgtt cttgattgac cggattgatc gcatacgcgc cgatgaacac
      781 gccggtcttg gcgcgcgcgg tatccgctcg atcgaactcg gactctttcg cggtcgcgct
      841 gacatacgct ttgactttgg tcgctcgatc aggcgtggta atccgatcga ctaacggatg
      901 ctccggtgcg agcaccatga aggtcgcgcc ccacaacgta tcgggcctcg tcgtgaagat
      961 ttccaatcga gcatcttcat tgcctagtcc atcgatcgag aaagtcacct gcgcgcctgt
     1021 gcttttaccg atccaattgc gttgcagtgt tttagttgtt tcagtgaagt cggctttcga
     1081 gtgatcgagc aatcgctccg catatttggt gatcctgaag aaccactgct cgagttcgcg
     1141 cttgatgact tcggtgccgc agcgctcgca ctggccatcg atcacttgtt cgtcggcgag
     1201 cacggtcaaa tctttgggac accaattgac cagcgaggtg gcgcgataag ccaagcccgc
     1261 tttgaacagt tgaataaaaa tccactgcgt ccatttgtag taacgcggat tggtcgtatc
     1321 gacttcgcgc gtccaatcga aggccgcgcc catgcgcttg atttgtgttt cgcggaagcg
     1381 cgtcatgttg cgcggcatga cgatggccgg attctcgccg agtttcaatg cgtagttctc
     1441 gccgtgaatg ccgaacgcgt caaagcccat cggttgaaag acatcgttgc cgatcatgcg
     1501 catatagcgt ccgaaagtat ccgcgccgct gtaagtatag a
//
LOCUS       DASRTJ010000305         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93212,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000305 DASRTJ010000000
VERSION     DASRTJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93212"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /gene="sat"
                     /locus_tag="VFF70_06220"
     CDS             <1..199
                     /gene="sat"
                     /locus_tag="VFF70_06220"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002472678.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZY44331.1"
                     /translation="TYYCKKCGSIVSSKTCPHDSSQHLIFSGTEVRRRLSAGEPLPVE
                     FTRPEVGQVLIDGLRAKQNGQ"
     gene            298..849
                     /gene="cysC"
                     /locus_tag="VFF70_06225"
     CDS             298..849
                     /gene="cysC"
                     /locus_tag="VFF70_06225"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZY44332.1"
                     /translation="MEVDHGCTLWFTGLSGAGKSTLAEIIEKELRGRGLRVEVLDGDV
                     VRTHLSKGLGFSKEDRDTNIRRIGWVCEVLTRNGVVAIAAAISPYHDIRDEIRSRIGR
                     FVEVYVEAPISVLAERDVKGLYKKALAGEIKHFTGVDDPYEAPLNPEVTCHSDGSETP
                     QQSALKIIRKLEELGYVPVAAAA"
     gene            complement(967..1971)
                     /locus_tag="VFF70_06230"
     CDS             complement(967..1971)
                     /locus_tag="VFF70_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZY44333.1"
                     /translation="MEFIVKDTIQKLVEAFGPVGYEDQVRSIIRKEIAGLADQISVSK
                     LGSLIAFKKGAGSGKKIMLAAHMDEIGIIVTHIDKHGFVRFATLGGVVALYSIAGRVQ
                     FANGQIGVIGLEHRDDTSKSPTLDQLYIDVGATDKSSCPVHVGDVAIFHRPLESIGSR
                     LTAKSLDDRIGCAVLIETFRRLKSTPHDIYFVFSVQEEVNSAGARTSAYSIEPDLALA
                     VDITGTGDTPKALPMAVELGKGPAIKVKDVAMIADPRIKEWMIKRAEAARIPYQLEIL
                     RLGTTDAEVIQSSRDGVPSGALSIPGRYLHSASEMVDLADVENAVKLLLALISKPIDL
                     "
     gene            complement(2010..3053)
                     /locus_tag="VFF70_06235"
     CDS             complement(2010..3053)
                     /locus_tag="VFF70_06235"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HZY44334.1"
                     /translation="MSFTSNLRQLSEAIGVSGDERAVREIVLRTIEDHVDEVRVDTIG
                     NVLAIKKARGKAKLRVMLDAHMDEIGLMIVGANDDGTLQFRPIGGIDDRILPGKVVLI
                     GPDKIPGVIGLPPIHLSRGDMTVKKFDALAIDIGASSRDQAIGAVRVGHTATFNTHYR
                     SLGAIVSGKAFDDRAGCAVLIEILQQPRLSNDILAAFTVQEEVGLRGARVAAYALEPD
                     AAIAIDATPANDLPPSIEKDVNPNTRLGDGPAIYISTRGSVSDPRLVKHAIDVAARGK
                     IPYQIRQPGGGGTDMGGIIPTRSGVPGISISVPTRYLHAPVGLLQVSDARHTIDLVRQ
                     TVAHLTPKTLRRQ"
     gene            complement(3124..4176)
                     /locus_tag="VFF70_06240"
     CDS             complement(3124..4176)
                     /locus_tag="VFF70_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZY44335.1"
                     /translation="MDISALLKKLSQANGISGHEAEIRSIVLDEFGRTADETRTDKVG
                     NAIAIKRGSQKSRRRRKIMLASHMDEIGLMVSGLSHGFLRVSKIGGIDPRVQLSQEVI
                     VHGRRDLPGVISSTPPHLLKAEDRTKIISIEQLWIDVGLSDRQATRSIRIGDLVSIRR
                     EVIELQNGLLAGKAFDNRASVAAVAVCLEQLSHVQHHWDVVAVATTQEEIGFVGATTA
                     AFGIAPDVAIAIDVTYARQNGSTGNEVFPLDKGPTISIGPNMHPRMTQRLIDTAKQIE
                     LDYQLEPMEGSSGTDGWAIQVTHAGIPTGIVSIPIRSMHTPVEVIAPKDVERTGRLLA
                     EFVAELDEEFYQTLND"
     gene            complement(4210..4779)
                     /locus_tag="VFF70_06245"
     CDS             complement(4210..4779)
                     /locus_tag="VFF70_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44336.1"
                     /translation="MSWLDGVSPHQRRLYTILIGIIAITLPCYCFGLGALALAPGIRS
                     SQIVVPTSTIAPTNTPSVATATPQPGITLSPSPTQWIPPSFTPTSTKTSTPTNTALPT
                     NTPTTTSTPTATTTNVPASNTPTATTTNVPASNTPTSTLTPTVPPSATPSVTNTPTET
                     ATPTDTATATATATDTATPVATIEPTVAP"
BASE COUNT         1031 a         1314 c         1437 g         1108 t
ORIGIN      
        1 cacttattac tgcaaaaaat gtggcagcat tgtctcttcc aaaacgtgtc cacatgattc
       61 gtcccagcat cttatcttta gcggcactga agtccgccgc cgtttgagcg cgggcgaacc
      121 cctgccggtg gaattcactc gaccggaagt gggtcaggtg ctgatcgatg gcttgcgcgc
      181 caaacaaaac ggacagtaga ttcgatcggg cgcgtaagat caatcgagta attgatagat
      241 cgcccagcgt tttgatcgaa tcgatcaatc agaaatcatt atagggaggc aggaaagttg
      301 gaagtcgatc acggttgcac actttggttc acaggtttgt caggcgcagg caagtccacg
      361 ctggctgaga ttatcgaaaa agaattgcgc ggacgcgggc tgcgcgtcga ggtattggac
      421 ggcgatgtgg tccgcacaca tttatcaaaa ggcctgggtt tcagcaaaga agatcgcgac
      481 acgaacattc gacgcatcgg ttgggtgtgc gaagtgctga cgcgcaacgg cgtggtggcg
      541 attgccgcgg cgatctcacc gtatcacgat attcgcgatg agatccgatc gaggattggc
      601 cgcttcgttg aggtgtatgt ggaagcaccg atcagtgttc tggccgaacg cgatgtgaag
      661 ggtttataca agaaggcgtt ggcgggcgag attaaacatt ttaccggcgt cgacgatccg
      721 tatgaggcgc cgttgaatcc cgaagtgacg tgtcattcag atggcagcga gacgccgcag
      781 caaagtgcgt tgaagatcat tcgtaaattg gaagagttgg gttacgtgcc ggtggctgcg
      841 gcggcgtaat cacacagact ttggtaaata aagatgcctc ccgcactgag aggcatcttt
      901 tgttttctgc acagcgacgt tccagtggaa tgtcccgaag acgccccgcc gggttgtcta
      961 tacgatttac aagtcgatcg gttttgagat caacgccagc agcaacttca cggcgttttc
     1021 aacgtccgct agatcgacca tctccgaagc gctgtgcaaa tagcggccgg ggatcgagag
     1081 cgcgccactg ggcacaccgt cgcgtgagga ttgaatgact tcggcatcgg tcgtgccgag
     1141 gcgcaaaatt tccaattgat agggaatgcg cgcggcctca gcgcgtttga tcatccactc
     1201 tttgatgcgc ggatcggcga tcatggcaac atctttgact ttgatcgccg gccctttgcc
     1261 caactccacg gccatgggca gcgccttggg tgtatcaccg gtacccgtga tatcgacggc
     1321 gagcgccaga tcaggctcga tgctgtaagc cgatgtgcgt gcgccggctg aattcacttc
     1381 ttcttgcacg ctgaagacaa agtaaatatc atgcggcgtc gacttcaagc ggcgaaatgt
     1441 ttcaatcaag accgcgcagc cgattcgatc gtccaggctt ttagcggtta gccggctgcc
     1501 gatggattcc agcggccgat ggaagatcgc gacatcgccg acatgaaccg ggcaggacga
     1561 tttgtctgtt gcgccgacat cgatgtacaa ctgatcgagc gtgggacttt tagatgtatc
     1621 gtcgcgatgc tccaacccga tcacgccgat ctgcccattg gcaaattgca cacgcccggc
     1681 aatgctatac aaagcaacca ctccacccag cgtcgcaaat cgcacgaaac catgtttgtc
     1741 gatgtgcgtc acgatgatgc cgatctcatc catgtgggcg gccagcatga tcttcttacc
     1801 gctccccgct ccttttttga atgcgatcag actgcctaat ttgctgacgc tgatttgatc
     1861 ggcgaggccg gcgatctctt tacggataat cgatcgaact tgatcttcgt agccgactgg
     1921 cccgaaggcc tcaactaatt tttgaatggt atctttcaca atgaactcca tgaacgtgcg
     1981 aggttttcaa aacctcgcac gtttttttat tactgtctac gcaacgtttt gggtgtcagg
     2041 tgtgccactg tctggcgcac cagatcgatt gtgtgacgcg catcgctcac ttgcaacaag
     2101 cccaccggcg cgtgcagata acgcgtgggg actgaaatgg atatgcccgg cacacccgat
     2161 cgggttggaa tgataccgcc catatctgtg ccgccgccgc cgggttgtcg aatttgatac
     2221 ggaatcttgc cgcgcgccgc tacatcgatc gcatgtttca ctaatctggg atcagatact
     2281 gatccgcgcg tcgaaatata aattgccggg ccgtcgccca atcgcgtatt cgggttgaca
     2341 tctttttcga tcgagggcgg cagatcattc gcgggcgtcg catcgatcgc aatggccgca
     2401 tcaggctcga gtgcatatgc cgcgactcgc gcgccgcgca gcccgacttc ctcttgcacg
     2461 gtaaaggccg cgagaatgtc gttcgataag cggggctgtt gcaaaatctc gatcagcacc
     2521 gcacagccgg cgcggtcatc aaatgctttg ccgctgacta ttgcgcccag cgatcgatag
     2581 tgcgtgttga aggtggccgt gtgaccgacg cggactgcgc caatcgcttg atcgcggctg
     2641 gatgcgccga tgtcgatggc taacgcatca aattttttca cggtcatatc gccgcgcgac
     2701 aaatggatgg gcggcagccc gataacgccg ggaattttat ccgggccaat gagcaccact
     2761 ttgccaggca gaatgcgatc gtcaatgcct ccgatgggcc ggaattgcag tgtaccgtca
     2821 tcgttcgcgc caacgatcat caagccgatc tcatccatgt gcgcgtccag catcacccgc
     2881 agcttagcct tgccgcgcgc cttcttgatc gccagcacat tgccgatcgt gtcgacgcgt
     2941 acttcgtcga cgtgatcttc gatcgtgcgc aggacaattt cgcgcacggc gcgttcatcg
     3001 ccggagacgc caatggcttc agaaagttgc cggaggttag aggtaaacga cattgtcaat
     3061 acttgtaggg caggcggtcg aagatcctag aaacgaagtg gataggaccg cacgccccga
     3121 cggttaatca ttcaatgttt gataaaactc ttcatctaac tcagccacaa attccgccaa
     3181 caagcggccg gtgcgttcga catctttggg cgcaatcacc tcgacgggcg tgtgcatcga
     3241 tcggatcggg atgctgacga tgccggtagg aatgccagcg tgcgtgactt gaatcgccca
     3301 accgtcagtg ccgctggaac cctccatcgg ttcaagctga taatcgagtt cgatctgctt
     3361 ggcggtatcg atcagacgct gtgtcatgcg cggatgcatg ttcggcccaa tgctgatcgt
     3421 tggtcccttg tccagcggaa aaacctcgtt gcccgttgat ccgttttgcc ttgcgtaagt
     3481 cacatcgatc gcaatggcca catcgggtgc tataccaaaa gccgccgtgg ttgcgccgac
     3541 aaatccgatt tcttcttgcg tggtggccac cgctacaacg tcccagtgat gctgcacatg
     3601 actcagctgc tccaggcaga ctgcgacggc cgcgaccgag gcgcgattgt cgaaggcttt
     3661 gcctgcgagc aacccgttct gaagttcgat cacttcgcgg cggatcgaaa ccagatcgcc
     3721 gatgcggatc gatcgggtgg cttgccgatc cgataagccc acatcgatcc acagctgctc
     3781 gatcgatatg attttggtgc gatcttcggc tttcaataag tgcggcgggg tgctgctgat
     3841 gacgccgggc agatcgcgcc ggccatgcac gatcacttct tgactcagct gcacgcgcgg
     3901 atcgatgccg ccgatcttcg agacgcgcag gaagccgtgg ctgaggcccg acaccatcaa
     3961 gccgatctcg tccatatgag aagcgagcat gattttgcgg cggcgtctgc ttttttggct
     4021 gccgcgtttg atcgcaatcg cgttgcccac tttatccgtg cgtgtttcgt cggcggtgcg
     4081 gccgaattcg tccagcacga tcgatcggat ttcagcttcg tggccggaga tgccgttcgc
     4141 ctgcgacaat tttttaagca gcgcgctaat atccataacg ggcatcgatc acttttcgga
     4201 gaatgagcat cacggcgcga cggtcggttc gatcgtcgcc accggcgtcg ccgtgtcagt
     4261 ggccgttgcg gtggcggtgg cggtgtctgt cggcgtcgca gtttcagtcg gcgtgttggt
     4321 cacgctgggt gtggccgatg gcggaacagt aggcgtcaga gtggatgtcg gcgtattgga
     4381 ggcgggcaca ttcgtcgtgg tcgcggttgg cgtatttgaa gcagggacat tcgtcgtggt
     4441 cgcggtcggc gtcgaggtgg tcgtcggcgt atttgtcggc agcgccgtgt tcgtcggtgt
     4501 tgatgttttg gtcgaggtcg gtgtgaacga tggcggaatc cattgagtag gcgatggact
     4561 gagcgtgatg cccggctgcg gtgtggccgt ggcgaccgag ggtgtattcg tcggcgcgat
     4621 ggtggatgtg ggcacgacga tctgactcga tcgaatgccc ggcgcaagcg cgagcgcgcc
     4681 caaaccaaaa cagtaacagg gcagggtaat ggcaatgatg ccgatcaaga ttgtatacaa
     4741 acgccgttga tgcggcgaga caccgtcaag ccaactcatg catgctccgc aataatgccg
     4801 atcgaagtga cggcgatgat agcaggttca atagggcttg tcaaacaaaa tgcaaggatg
     4861 tgctagctgc aacaacaaga agcccgatca
//
LOCUS       DASRTJ010000306         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_272782,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000306 DASRTJ010000000
VERSION     DASRTJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272782"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFF70_06250"
     CDS             <1..366
                     /locus_tag="VFF70_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44337.1"
                     /translation="IFARVRRKTRLAARRLRGIEQPYPDIADIMDDISNISPAQRAFM
                     AIQSQALQAYVPRSYPGRVAVFRTRAQSLVCSFDPLMKWATLAGDRVDVRIVEGSHHN
                     MLQEPYVRSLAVQLKNSLV"
     gene            392..553
                     /locus_tag="VFF70_06255"
     CDS             392..553
                     /locus_tag="VFF70_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44338.1"
                     /translation="MGWEKLILGGVETIRIKGTHDELLRSARQVAQVAQHLKAHLAAL
                     PPRRSWNTF"
     gene            667..2022
                     /locus_tag="VFF70_06260"
     CDS             667..2022
                     /locus_tag="VFF70_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZY44339.1"
                     /translation="MMTALDRSHLENLLEQEEQRFSHDHPRSRNLFERAQKSLLAGVP
                     MNWMVKWAGSFPVFVKAGRGAHFTDVDGREYIDLCLGDTGAMTGHAPEGSTAAIVDQI
                     KRGITFMLPSEDAIWVGEELSRRFGLPYWQIAMTATDANRFSIRLARAITGRPKILVF
                     NWCYHGTVDETFAVLKNGQVGPRPGNLGPAVDPAITTKVVEFNDIDALEKSLAARDVA
                     CVLAEPVMTNIGIIHPDPGYHAALRDLTRQTGTLLIIDETHTICTGPGGYARAYHLEP
                     DMFVIGKPIGSGIPGAAYGFSEEVTQRIKDGTDFETSDTSGIGGTLSGNALALAAMRA
                     TLQNVLTPEAYDRMIPLAEKFEAGVEAVIKEYELPWIVKRLGCRAEYWFRPTPPRNGA
                     EAAAAIDAELDRFMHLFALNRGILLTPFHNMALISPATAVADIDYHTQVFRAAVKALI
                     G"
     gene            2078..>3075
                     /locus_tag="VFF70_06265"
     CDS             2078..>3075
                     /locus_tag="VFF70_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZY44340.1"
                     /translation="MKSQTLIQLLADRVQQQPESIAIEALDRDPLTYRGLYRHVISIG
                     AMLNQAGIGRNDRVAIVAPNGPEMATAFLSVAAGATSAPLNPQYRAAEFDFYLSDLKA
                     KALIIQADLESEARTVALDRGIPIIDLVPRLDCAAGQFELLPHTSCPIKPGDFVAADD
                     IALVLHTSGTTSRPKLVPLTQANICASALHVSASLQLSSADRCLNVMPLFHIHGLIAA
                     VLASLSAGGSVLCTPGFQAPSFFEWFDRFRPTWYTAVPTMHQVILARAGDQLALLRRS
                     ALRFIRSSSASLAPVVMNELERTFKVPVIEAYGMTEAAHQMASNPLPPLPRKAGSVG"
BASE COUNT          630 a          846 c          870 g          729 t
ORIGIN      
        1 atatttgccc gtgtccggcg caaaacgcgg cttgccgcgc gacgtctgcg cgggatcgaa
       61 cagccctatc cagacattgc cgatatcatg gacgacatct ccaatatttc gccagcgcag
      121 cgcgcgttta tggcgattca atcccaagct ctgcaagctt acgtcccgcg gtcatatcca
      181 ggccgcgttg cggtatttcg cacgcgcgct caatcgctgg tgtgttcgtt cgatccgctg
      241 atgaaatggg ctacactggc tggcgatcga gtggatgtcc ggatcgtcga aggttcgcat
      301 cacaacatgc tgcaagagcc ctacgttcga tcgctggccg tgcagttaaa gaatagtctg
      361 gtttaaccac accgcattta ggtttcaaaa aatgggctgg gaaaaactga tactgggcgg
      421 cgtggaaacg attcgaatca aaggcacgca cgacgaattg ctgcgctccg ctcggcaagt
      481 ggcgcaggtc gcccaacact tgaaagcgca cttggccgca cttccgccgc gccgatcatg
      541 gaacacattc tgacgttgaa gtgataaccg aagcgcgaac gatgaataat cgctcgcgct
      601 ttttgttagt cgaggcgttt gtgttaaact ggtgccgtaa tttcctgtga tttctttgga
      661 gaataaatga tgactgctct cgatcgatct catctcgaaa atctgctcga gcaggaagag
      721 cagcgcttca gtcacgatca tccgcgctcg cgaaatttgt ttgaacgcgc ccagaagtca
      781 ttgttagccg gtgtgccgat gaattggatg gtgaagtggg ccggctcatt tcccgtcttc
      841 gttaaggcag gacgtggcgc gcacttcact gacgtggatg gccgcgaata tatcgatctg
      901 tgcctgggcg acacgggcgc tatgaccggt catgctccag aaggatcgac ggctgcgatc
      961 gtggatcaga tcaagcgcgg catcaccttc atgctcccta gcgaagatgc catctgggtc
     1021 ggtgaagaac tgtcgcgccg ctttggtttg ccgtactggc agatcgcgat gaccgccacc
     1081 gatgcgaatc gcttttcaat tcggctcgcg cgcgcgatca ccggccgtcc gaaaattttg
     1141 gttttcaact ggtgttatca cggcacggtc gatgagactt tcgccgtttt gaaaaacggg
     1201 caggtcggac cgcgaccggg caatttaggt ccagcggttg atcccgcgat caccacgaag
     1261 gtggttgagt tcaatgatat cgatgcattg gaaaagtcgt tggctgcgcg tgatgtcgct
     1321 tgcgttttgg ctgaacccgt catgaccaac atcggcatca ttcatcctga tccgggctat
     1381 cacgcagcgc tgcgtgacct cacccgccag acgggcacgc tgttgatcat cgacgagacc
     1441 catacgatct gcaccggacc gggcggctac gcgcgcgcgt atcacttgga gccggacatg
     1501 ttcgtgatcg gcaaaccgat cggcagcggc atccccggcg cggcttacgg cttctctgaa
     1561 gaagttacac agcgcattaa agacggaacg gattttgaaa catcagacac cagcggcatt
     1621 ggcgggacat tgtccggcaa tgccttagcc ctggcggcca tgcgcgcgac attgcaaaat
     1681 gttttaacgc cagaggcata tgatcgcatg attccgttgg cggaaaaatt cgaggcaggt
     1741 gttgaagccg tgatcaagga atacgaattg ccgtggattg taaaacgcct gggctgccgc
     1801 gccgaatact ggttccggcc cacgccgccg cgcaacggcg cggaggccgc cgccgcgatc
     1861 gatgctgagc tcgatcggtt catgcatttg ttcgcgctca atcgcggcat cttgctgacg
     1921 ccgtttcaca acatggcact gatctcgccg gccaccgcgg ttgccgatat cgattatcac
     1981 acgcaagttt ttcgtgcagc agttaaggcg ctgatcggtt gattgatttg aatgccatgc
     2041 gaaaacgttg tgatcgtact tatttgatgg agcgattatg aaatcccaga ccctgatcca
     2101 attgctggcc gatcgggtcc aacagcagcc cgagtctatt gcgatcgagg cgcttgatcg
     2161 tgatccgtta acctatcgag gcttgtatcg gcacgtgatc tcgatcgggg ctatgcttaa
     2221 ccaggcgggc atcggtcgca atgatcgagt agccatcgtg gcaccgaatg gccctgaaat
     2281 ggccactgcc ttcttatccg tggcggcagg cgccaccagt gcgccgctca atcctcagta
     2341 tcgcgctgct gagtttgatt tttatttgtc cgatttgaag gcaaaagcac ttatcattca
     2401 ggctgatctg gaatccgaag cgcgaacggt ggcgctcgat cgcggcatcc cgattatcga
     2461 tctcgtcccg cggctcgatt gcgcggccgg acaatttgaa ttactgccgc acacttcatg
     2521 tccgatcaag cctggtgatt ttgtggccgc tgacgacatc gcattggtgt tacacacgtc
     2581 tggcacaacc tctcgaccta agcttgtgcc tttaacgcag gccaacattt gcgcgtccgc
     2641 tctgcatgtg agtgccagct tgcagctcag ctctgcggat cgctgcttga atgtgatgcc
     2701 gctgtttcat attcacgggt tgatcgcggc tgtcttggct tcactttctg ccggtggaag
     2761 cgtgctgtgc acaccgggtt ttcaggcgcc tagcttcttt gaatggttcg atcggtttcg
     2821 cccgacatgg tatacggctg ttccgacaat gcatcaagtg attctcgccc gggctggcga
     2881 tcaactagcg ctgctccggc gatcggcctt gcgctttatt cgatcatctt ctgcctcgtt
     2941 ggctccagtg gtgatgaatg aattggagcg gacgtttaag gtgccggtga tcgaagccta
     3001 tggcatgact gaagctgccc atcaaatggc cagcaatccg ctgccaccac tgccgcgcaa
     3061 agcgggctcc gtggg
//
LOCUS       DASRTJ010000307         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_159103,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000307 DASRTJ010000000
VERSION     DASRTJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159103"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFF70_06270"
     CDS             complement(<1..1155)
                     /locus_tag="VFF70_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44341.1"
                     /translation="MSPEIDWQVQDDHGQEEIVHTLQPQAPRWRPIAIGVVILIGVAL
                     GVVYRSIPEPPTPPIPTPIPTPPITQTIDQESQALAFGRMQAFIDLQDPGDSIWQQKQ
                     SLAFRAWGRPATGDLYTIVESGTLSGDRVWADVIQFQDDEYFRQTRFYQLRNNHWRRI
                     APPIDAAFWGEEQTAQTQHFDLVYRAQDAVGAHLLADQLEAFYQRVCQDVECDAVKSS
                     NNQRLTLVLLPDIATTASLGGTNFSVPITYALPSARLTGLYFQDLTAEIPDQNQLSNA
                     DDYGYIESTLLASIVFAATGDPERWANTQDGSILVGMIANWEQARIEPNQLGYDDTYW
                     RNVLSANQTQLPLEALWTSWPPTYTQAVNELMWAESSALIKFMDQHYGAKT"
     gene            complement(1195..>1702)
                     /locus_tag="VFF70_06275"
     CDS             complement(1195..>1702)
                     /locus_tag="VFF70_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44342.1"
                     /translation="QSLYDSAPGNDEPLVQMAIDKLYVPHLVAKIASGRLALLSDFKK
                     GDLFVGNITQWEINRITPISALSDAQIFSTLQNVNDQDLLPLDVLWDAIPPYDLPQSQ
                     LDMLVTETWSVIRFIETKYGSDGVVHFLNAIGPAQSMSDAIENGLSVSYADFDREWRA
                     WLKTQLSK"
BASE COUNT          391 a          428 c          465 g          418 t
ORIGIN      
        1 ggttttggct ccatagtgtt gatccataaa cttgatcaac gcggaagatt cggcccacat
       61 caattcgttg accgcctgag tatacgtcgg cggccaggaa gtccacagcg cttcgagtgg
      121 cagttgggtt tgattggcgc tgagcacatt tcgccagtaa gtgtcatcgt aaccaagttg
      181 gttcggttca atgcgagcct gttcccagtt cgcaatcatc ccgaccagta tggacccatc
      241 ttgggtattt gcccagcgtt ctggatcacc cgtagctgca aatacaatcg aagcgagcaa
      301 ggtactctcg atatagccat agtcatccgc gttgctgagt tgattttgat ctggtatctc
      361 ggcagttagg tcctgaaaat ataaacccgt gaggcgcgcc gacggcaaag cataggtaat
      421 tggcacactg aagtttgttc cacctaggct ggcggtggta gcaatatcag gcagaagcac
      481 cagcgtcagc cgttgattgt tcgaagattt aaccgcatca cattctacgt cttgacatac
      541 ccgctggtag aatgcttcaa gctgatcggc taagaggtga gccccgaccg catcctgggc
      601 tcgatagacg agatcgaaat gctgagtctg tgccgtctgc tcttctcccc agaaggctgc
      661 gtcgatcggc ggcgcgattc gacgccagtg attattgcgc aattgataga agcgcgtctg
      721 gcggaaatat tcatcgtctt ggaactgaat cacatcggcc cagactcgat caccggacag
      781 cgtgccgctc tcgacgatgg tgtatagatc gccagtggcc ggtcgtcccc aagccctgaa
      841 cgccagcgac tgtttttgtt gccagatcga atcgccaggg tcttgcaagt ctataaaagc
      901 ctgcatgcgg ccgaaggcta gcgcctgtga ttcttgatcg atcgtttggg tgatcggcgg
      961 agtgggaatc ggtgtcggga ttggtggtgt gggcggttcg ggaatcgatc gatagaccac
     1021 gcccaacgca acgccgatga gaatcaccac accgatcgcg atcggtcgcc agcgcggcgc
     1081 ttgaggctgc aaggtatgaa cgatctcttc ctggccgtga tcgtcttgaa cttgccaatc
     1141 gatctcagga ctcatcggtt aaaggagaaa ggcgaaagga aaaatttata gcgttcattt
     1201 tgatagttgt gttttcaacc acgcgcgcca ttctcgatcg aagtcggcgt agctaacgct
     1261 caatccattc tcgatcgcat ccgacatcga ctgcgcgggg ccgatcgcgt tgaggaaatg
     1321 cacgacgccg tctgagccgt attttgtttc gatgaatcga atgaccgacc aagtttcagt
     1381 gactaacata tcgagctgtg attgtggtag atcataaggc ggtatcgcat cccacaacac
     1441 gtcgagtggc agcaaatctt gatcattgac gttttgcagt gttgaaaaga tctgtgcatc
     1501 gctcaacgct gaaattggtg tgatgcgatt gatctcccac tgcgtaatgt tgccaacaaa
     1561 gagatcgcct ttcttgaaat cggacaagag tgctagcctg cccgatgcaa ttttggcgac
     1621 taggtgcggc acatataact tatcaatcgc catctgcacg aggggttcat cgtttcccgg
     1681 cgcgctatcg tagagcgatt gg
//
LOCUS       DASRTJ010000308         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93334,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000308 DASRTJ010000000
VERSION     DASRTJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93334"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFF70_06280"
     CDS             <1..636
                     /locus_tag="VFF70_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZY44343.1"
                     /translation="IKVPCPGPFTLAGRIAPGKIYRARMDVAQGLSEIINAELQALVA
                     AGVDFIQLDEPSYAVHPDQPRAFVDLFNQTVQGVNARIGLHLCFGNFVGRPVARRTYQ
                     PLFPYILDTHTDELALEFANRELAEIELGQQVAAAGKTLAAGLIDVKNYYIETPEDVA
                     DRIRTALKYVPADKLTIVPDCGFSQTARWASRAKLKAMVEGTQIVRRELAG"
     gene            646..1566
                     /locus_tag="VFF70_06285"
     CDS             646..1566
                     /locus_tag="VFF70_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HZY44344.1"
                     /translation="MAFIRTVRGDIDPSDFGVCYPHEHVLCTPPANVTDRDLTMDSEA
                     TAIKELTWFHEAGGRSLVEMTPADYGRNAAGLKRVSEQSGVHIVCMTGHHKEAFSAAW
                     VQDRSIEELADRFVRDATKGVDGTSIKAGAIKVASSLNKITPNEEKVFRAAARAHQMT
                     GAPISTHTEAGTMALEQIDLLRSEGVDPSRVIIGHLDRNLDWEYHLQIARTGVYIGYD
                     QISKEKYEPDSKRIEFILRLVKEGFGQQILLGGDLARKSYWPSYGTGGGPGLTYILWR
                     FVPWLRSEGLSEHAINDILIRNPARAFSMG"
     gene            1623..2327
                     /gene="deoC"
                     /locus_tag="VFF70_06290"
     CDS             1623..2327
                     /gene="deoC"
                     /locus_tag="VFF70_06290"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZY44345.1"
                     /translation="MRQQLSSQTITYEELAKVIDHSLLKPELTESDVIAGCELAKQYH
                     VASVCVKPCDVKLAAKQLTGSDVAVGTVIGFPHGNSTTAIKVAEAQQALADGAIELDM
                     VINVGALRSGKTDYVRNEIAEVVKVAHAAKAIVKVIFENAYLADEQKVSACKICDEVG
                     ADFVKTSTGFALTGATIADVKLMRANVGPQVQVKAAHGVRTLDALLEMIDAGVTRSGA
                     TATAAMLDEFKRRHVG"
BASE COUNT          567 a          596 c          668 g          499 t
ORIGIN      
        1 atcaaagtac cgtgccccgg cccattcaca ctcgcgggtc gcatcgcgcc gggaaaaatt
       61 tatcgcgctc gaatggatgt ggcgcaggga ctgtccgaga tcattaacgc ggaactgcaa
      121 gcgctcgtcg ccgcaggcgt ggatttcatc caactcgatg agccttcata tgccgtacat
      181 cctgatcagc cacgcgcctt cgtcgatcta ttcaatcaaa cggtgcaagg cgtgaacgcg
      241 cgcatcggtt tgcatctgtg cttcggcaat tttgtcgggc ggcccgttgc gcgtcgaacg
      301 tatcaaccgc tttttccgta tatcctcgat acgcacaccg acgaattggc actcgaattt
      361 gccaaccgtg aactcgccga gatcgaattg ggtcaacaag tcgccgcagc gggcaagact
      421 ctagccgcag gattgatcga tgtgaagaat tactacatcg agacgccgga agatgtagcc
      481 gatcggattc gaactgcact caaatatgtg cccgctgata agttgacgat tgtgcccgat
      541 tgtggcttca gtcaaaccgc gcgttgggct tcacgcgcga agttgaaggc tatggtggaa
      601 ggcacgcaga tcgtgcggcg agaactggca ggttaaggag atcgaatggc atttattcga
      661 accgttcggg gcgatatcga tccatcagac ttcggggtgt gctatccgca cgagcatgtc
      721 ctatgtacgc cgcccgcgaa cgtaaccgat cgcgatctca ccatggatag tgaagcaaca
      781 gcaatcaaag aactgacttg gtttcatgaa gccggtggtc gatcgttggt cgagatgaca
      841 ccagccgatt atggccgcaa tgccgccgga ttaaaacgtg tttctgaaca gtccggcgtg
      901 catatcgtgt gtatgacggg tcatcacaaa gaggcctttt cggcagcgtg ggttcaagat
      961 cgatcgatcg aggaactggc cgatcgcttt gtgcgggacg cgacgaaagg agtagatgga
     1021 acttcgatca aggccggagc gatcaaggtg gcatcgagcc tgaataagat cactccaaac
     1081 gaagagaagg tatttcgagc ggctgctcgc gctcatcaaa tgaccggtgc gccgatctcc
     1141 actcacacgg aagccggtac gatggcgctg gagcagatcg atctccttcg atccgaaggc
     1201 gtcgatccat cgcgcgtgat catcggtcat ctcgatcgca atctcgattg ggaatatcac
     1261 ttacagattg cacggacggg cgtttatatc ggttacgatc aaatcagcaa agagaaatac
     1321 gaaccggata gcaaacgaat cgaattcatt ctgcgtttag tgaaagaggg cttcggccag
     1381 caaattttac tcggcggcga tctcgcccgc aagtcttatt ggccgagcta cggcacaggc
     1441 ggcggtccgg gtctcactta tattctgtgg cgcttcgtcc cgtggctgcg atcggaagga
     1501 ttgagcgaac atgcgatcaa tgacattctc atacgtaatc cggcgcgggc gttttcgatg
     1561 ggctgaggcc tgtacaaact atccgtgttc gagaactcga tcaatttaag gaagaaagcg
     1621 aaatgagaca acaactgagc agtcagacaa ttacgtatga ggaattggcg aaggttattg
     1681 atcattcact gctaaaacca gagctgaccg aatcagatgt gatcgccggc tgcgaactgg
     1741 ctaagcagta tcatgttgcg tccgtgtgtg tgaagccgtg cgatgtcaaa ctcgccgcga
     1801 agcagctgac cggatccgat gtcgcagtcg gcaccgtgat cggttttccg cacggcaatt
     1861 cgaccacggc gatcaaagtc gctgaagcgc agcaagcgct ggccgatggt gcgatcgagt
     1921 tagatatggt gatcaacgtc ggcgcgctgc gctcaggcaa gaccgattac gttcgcaatg
     1981 agatcgcgga agttgtgaaa gttgcgcacg cggccaaagc gatcgtcaaa gtgatctttg
     2041 aaaatgcgta tctggccgat gaacaaaaag tctcagcctg caagatatgc gatgaagtcg
     2101 gtgcggactt tgtcaaaacc tcaaccggct ttgcattgac cggcgcgacg attgccgatg
     2161 tgaaactcat gcgcgccaac gttggaccgc aagtgcaggt gaaggcagcc cacggtgtgc
     2221 gcacgcttga cgcgctactc gagatgatcg atgccggtgt cacacgatca ggcgccactg
     2281 ccactgccgc gatgctcgat gaattcaaac ggcggcacgt aggataagtg
//
LOCUS       DASRTJ010000309         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_320984,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000309 DASRTJ010000000
VERSION     DASRTJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320984"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFF70_06295"
     CDS             complement(<1..1241)
                     /locus_tag="VFF70_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZY44346.1"
                     /translation="MNKIYLSLAIHNHQPVGNFDFVFEEAYTKAYEPMIALLEKHPGV
                     RLALHYSGPLRDWLKAHRPEFMRRVRSLVGRKQIEIMTGGYYEPVLSALPDADKIGQV
                     QKMTEAIQTDFAYKPIGLWLAERVWEPQLPKSLREANIDYTIVDDTHFKNAGYTDNDL
                     FGYYVTEEQGQPLNIFATSKQLRYALPWTPVEEVIQWLRDQADRSLLPGAPQRVAVMG
                     DDGEKFGMWPTTFEHCWAGGWMDALFTALEQNSDWLITCPPAEVLDLIAPLGRVYLPV
                     ASYDEMGEWSLPAPLSREFAQVKERLQIEGREDVLRFLRGGMWRSFMVKYPEINTLHK
                     RMLWASERVHRMKKAAARSEALDHLWASQCNCPYWHGVFGGVYLFHIREANYAHLIEA
                     QNIADRASHPAETWIEVEQVDV"
     gene            complement(1354..2616)
                     /locus_tag="VFF70_06300"
     CDS             complement(1354..2616)
                     /locus_tag="VFF70_06300"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZY44347.1"
                     /translation="MHALAMILAGGRGSRLSILSQKRAKPAVPFAGKYRIIDFPLSNC
                     SNSGITTVGVLTQYRPRSLNDHIRTGAPWDLDRAHGGVWLLQPYERSTEMERYAGTAN
                     AIQLNFDFIKHNQPDFVVILAGDHIYKMNYEDMITFHTDHRADLTIGTLQVPLEEASR
                     FGLLETDANYRVSSFVEKPLQPKTTNASMGIYVFTTAVIDKILREDEADPNSAHDFGK
                     NIIPKMIESYKVYAYPFNGYWVDVGTVQAYWEAHMDLLADSPPLDLLDRNWIIHTRSE
                     ERPPVNIRTGAVVAHSLITDGCVIEGTVEYSVLSPGVKVARGAVVRNSIILTDTVIES
                     GAVIDRSIVDKDCVIRSNVVIGSGVDYSPNPIGDLTSGISLVGKGAEVPPNIKIGRNC
                     IIAGDVRAQDFSGTVVPSGATIGIVPPS"
     gene            complement(2706..>2993)
                     /locus_tag="VFF70_06305"
     CDS             complement(2706..>2993)
                     /locus_tag="VFF70_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HZY44348.1"
                     /translation="RYGSLPIVRATGGLKDSVLDAGRYPKRGTGFLFEPYSPAGLIGA
                     VDRAIKVFANTERWVEIQQRAMAADFSWQASAQQYEELYRRAIEFHQKTNQ"
BASE COUNT          670 a          851 c          802 g          670 t
ORIGIN      
        1 acatcaacct gctccacctc gatccaagtt tcagccggat gtgacgcgcg atcagcgatg
       61 ttctgcgcct cgatcagatg cgcgtagttc gcttcgcgga tatggaacaa ataaactccg
      121 ccgaagacgc cgtgccagta cggacagttg cattgcgacg cccacaaatg atcgagcgct
      181 tccgatcgag cggcggcctt cttcatgcga tgcacgcgtt cgctggccca cagcatccgc
      241 ttgtgcagtg tgttgatttc gggatatttc accatgaacg atcgccacat accgccgcgc
      301 aggaaacgca gcacatcttc gcggccttcg atttgcagac gctctttcac ttgagcgaac
      361 tcgcgcgaca acggcgcggg cagtgaccat tcgcccattt catcgtacga ggccacgggc
      421 aaatacacgc ggcctaacgg cgcaatcaga tccaagactt cggccggtgg gcaagtgatc
      481 aaccaatcgg aattctgctc cagcgcggtg aagagcgcat ccatccagcc gcctgcccaa
      541 caatgctcga aggtcgtcgg ccacattcca aatttctcgc cgtcatcacc catcaccgcc
      601 acacgctgcg gcgcgccggg cagcagcgat cgatcggcct gatcgcgcag ccattgaatg
      661 acttcctcga ccggcgtcca cggcagcgca taacgcaatt gttttgaggt cgcaaaaata
      721 ttcagcggct ggccctgctc ttccgtcacg taataaccga agagatcatt gtcggtgtaa
      781 ccggcgttct tgaaatgcgt atcgtcgacg atggtgtagt cgatgttcgc ttcgcgcagc
      841 gatttgggca gctgcggctc ccagactctt tcggccagcc acaacccgat cggcttgtaa
      901 gcgaaatcgg tttggatcgc ttcagtcatc ttttggacct gtccgatctt gtcggcatcc
      961 ggcaaagcgc tcagcaccgg ctcgtaataa ccgccggtca tgatctcgat ctgtttgcgg
     1021 ccgacgagcg atcgaacgcg gcgcataaat tcaggacgat gcgctttgag ccagtcgcgc
     1081 agcgggccgc tgtaatggag cgccaagcgc acgccgggat gtttttccag caaagcgatc
     1141 attggctcat aggctttggt gtaggcttct tcgaacacga aatcaaaatt gccgaccggt
     1201 tgatggttgt gaatagcaag cgagagatag attttgttca tgaatgttca ccgagtcaaa
     1261 atacaagtga ttgatgcgtg atttgtaaaa ttagtgatta gcaattactg agtcgcgggt
     1321 cgctggccgc aattacccat tactcattac tcgttagctg ggcggcacaa tgccgatcgt
     1381 cgcgccgctg ggcacgaccg tgcctgaaaa atcttgggcg cgcacatcac cagcgatgat
     1441 gcagttgcgg ccaatcttga tattcggcgg aacttccgcg cccttgccga cgagtgaaat
     1501 ccccgaagtg agatcaccga tcgggttcgg gctgtaatcc acgccgctgc cgatcacgac
     1561 attcgagcga atgacgcaat ctttatcgac aatcgatcga tcgatcaccg cgccggactc
     1621 gatcacggta tcggtcaaga tgatcgagtt gcgcacaacg gcgccgcgcg ccactttcac
     1681 gccgggcgaa aggacgctgt actcgacggt gccttcgatc acacaaccat cggtgatcaa
     1741 actgtgcgcc acgaccgcgc cggtgcgaat attgacgggc ggccgctctt ccgatcgagt
     1801 gtggataatc caattgcgat cgagcagatc gagcggcggt gaatcggcca gcagatccat
     1861 gtgcgcttcc caataagcct gcaccgtccc gacatcgacc caatagccgt tgaagggata
     1921 agcatagacc ttgtaggatt caatcatttt gggaataatg tttttgccga agtcatgcgc
     1981 ggaattggga tcggcttcat cttcgcgcaa gatcttatct atcaccgcag ttgtaaagac
     2041 atagataccc atcgacgcat tcgtcgtctt gggttgcagc ggtttctcaa caaacgaact
     2101 cacgcgataa ttcgcatctg tctccaacag cccgaagcgc gaggcttctt ccaagggaac
     2161 ttgcagcgta ccgatcgtca agtcggcacg atggtcggtg tggaaggtga tcatgtcttc
     2221 gtagttcatc ttgtagatgt gatcacccgc caggatgacg acgaagtccg gttggttgtg
     2281 cttgatgaaa tcgaagttga gttgaatggc gttcgcggtg ccggcgtagc gctccatttc
     2341 ggtcgatcgc tcgtacggct ggagcagcca cacaccgcca tgcgctcgat ccaagtccca
     2401 cggcgcccca gtgcgaatgt gatcgttcaa cgatcgcggg cgatactgcg tcaacacgcc
     2461 gacggtggtg atgccggagt tgctgcagtt cgacagcgga aagtcgatga tgcgatactt
     2521 gcccgcgaac ggcactgcgg gtttggcgcg tttttgagag aggatgctga gacggctgcc
     2581 ccttccaccc gctaagatca tcgccaatgc atgcatagtt gatctctcta gaagtttttt
     2641 cgtacacgaa catcacgaat cacacatata gaatttgaat tcgtgatatt cgtgcgattc
     2701 gtgtactact gattagtctt ttgatgaaac tcaatcgccc gccgatacaa ctcttcatac
     2761 tgctgcgccg acgcctgcca cgaaaaatcg gcggccatgg cgcgctgttg gatttcaacc
     2821 caacgttctg tgttggcaaa tactttgatc gcgcgatcca cagccccgat caagcctgct
     2881 ggcgaatacg gctcgaataa aaagccggtg cctctttttg gataacgtcc cgcatctaaa
     2941 accgaatctt tcaagccgcc cgtcgcgcgg acgatcggca gagagccata ccg
//
LOCUS       DASRTJ010000310         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_56301,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000310 DASRTJ010000000
VERSION     DASRTJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56301"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFF70_06310"
     CDS             complement(<1..328)
                     /locus_tag="VFF70_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HZY44349.1"
                     /translation="MITSLTNDKVKFIRALSERKNRQREGRFVVEGARLIDEALAAQL
                     RPDWIFCAEKIPTRAKQTLNHLRSINVEVIDVSDAVLKACSDTETPQGLIAVLPFPRL
                     DPPPDPK"
     gene            complement(371..724)
                     /gene="rplT"
                     /locus_tag="VFF70_06315"
     CDS             complement(371..724)
                     /gene="rplT"
                     /locus_tag="VFF70_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZY44350.1"
                     /translation="MPRAKGGPHLRRKHNKILKQTKGQKGARHRLWKKGSEARLHSLY
                     YSYRDRRARARDFRKLWIARINAAARISGLSYSKFMFGLKKAGVTIDRKVLSDLAVRD
                     ASAFAQLVQVAKDAK"
     gene            complement(882..1091)
                     /locus_tag="VFF70_06320"
     CDS             complement(882..1091)
                     /locus_tag="VFF70_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HZY44351.1"
                     /translation="MPKIKTHKATSKRFRVTGTGKIRRTRLGKSHLRRRKSARAKAEF
                     GELKTVTNKAMARRVRRLAPYLKKK"
     gene            complement(1057..1587)
                     /gene="infC"
                     /locus_tag="VFF70_06325"
     CDS             complement(1057..1587)
                     /gene="infC"
                     /locus_tag="VFF70_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZY44352.1"
                     /translation="MIGADKQHVGITPIQEALRMAREAQLDLVEISPQADPPVAQIMD
                     FGKFLYEQHKKEREAKKAQKVIEVKEIQLRPKTDPHHRGFKVRDARKWLTDGMKVKVR
                     VRFRGREIAYPELAREDLASVADELADIAVVEQRPNMEGRTMLMVLAPSGKAPKKPTV
                     KKETESAKDQNPQSNQ"
     gene            complement(1617..1781)
                     /locus_tag="VFF70_06330"
     CDS             complement(1617..1781)
                     /locus_tag="VFF70_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44353.1"
                     /translation="MLRSARNGGSIGVLTDQSLYVIILACTMPPGAGSPGGSNKSTED
                     KHTSPQKTIA"
     gene            complement(1858..>2070)
                     /locus_tag="VFF70_06335"
     CDS             complement(1858..>2070)
                     /locus_tag="VFF70_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZY44354.1"
                     /translation="IRSEVDESTDRMQAKIRNAQLLKIPYMLVVGDKEQEADAVAIRL
                     RSGEDLKAKPLAEFVGMAQKAIESKE"
BASE COUNT          396 a          562 c          567 g          545 t
ORIGIN      
        1 gtttcgggtc gggcggcgga tccaatcggg gaaagggcag aaccgcgatt aagccctgcg
       61 gcgtttctgt atcgctgcaa gctttcagca ccgcatccga cacatcgatc acttcgacat
      121 tgatcgatcg caagtgattg agcgtttgtt tggcgcgcgt cggaattttt tcggcgcaga
      181 agatccaatc gggtctgagc tgggccgcca gggcctcatc gatcaaacgc gcgccttcaa
      241 caacaaagcg cccttcccgc tggcgatttt ttcgctcgga aagggcacgg ataaatttga
      301 ctttgtcgtt cgtgagactg gtaatcatgt aaagaaagtg cgacgcattt ggcaggtgcg
      361 tcgcacattt ttatttcgcg tctttagcga cttggaccaa ctgggcgaag gcggatgcat
      421 cgcgcacggc cagatcggac agcactttgc gatcgatcgt cacgccggct ttcttcaggc
      481 cgaacatgaa cttgctgtac gacaagccgc tgatgcgcgc cgccgcgttg atgcgcgcga
      541 tccacaattt gcgaaaatcg cgagcgcggg cacgtcggtc gcggtaagag taatacagcg
      601 agtgcagccg cgcttcactg ccctttttcc acaggcgatg gcgcgcgcct ttttgacctt
      661 tggtttgctt gaggatcttg ttgtgtttac ggcggagatg tggcccacct tttgctcgag
      721 gcatttttat attttccttc ttgaaaagat ttaccctcgg tccgaagacc tacggggagg
      781 ttaagttctg atgagtaatg agtgattagt aaatggaatt acgcacacct gcactgcaac
      841 gcgcgcagtg cggtgcaagt gtcactcgtt actcattaga tttacttctt cttcaaatat
      901 ggagctaagc gtctgacgcg gcgcgccatg gctttgttcg tcaccgtctt gagttcgccg
      961 aattcggctt tggcgcgggc ggacttgcgg cgtcgcaagt ggctcttgcc taatcgggta
     1021 cggcggatct tgccggtgcc agtcacgcga aagcgtttac tggttgcttt gtgggttttg
     1081 atctttggca ctttctgttt ccttcttcac agtaggtttc ttgggcgctt tacccgacgg
     1141 ggccagcacc atcaacatcg tgcggccttc catgttcggg cgttgttcga caactgcaat
     1201 gtcagctaat tcatcggcca cactcgccag atcttcacgc gccaattccg gataggcaat
     1261 ttcacggccg cggaagcgca cgcgcacttt cactttcatg ccatctgtca gccacttacg
     1321 cgcatcgcgc actttgaagc cgcgatggtg cggatcggtt ttagggcgca gttgaatctc
     1381 tttgacctcg atcactttct gcgccttctt ggcttcgcgt tccttcttgt gctgctcgta
     1441 taggaatttc ccgaagtcca taatctgcgc gacgggcgga tcggcttgcg gtgaaatctc
     1501 aaccagatcg agttgagctt cgcgcgccat gcgcagtgct tcttgaatcg gcgtaatgcc
     1561 gacgtgctgt ttatcagcgc cgatcaatcg gacttcttta acgcgaatgc cttcgttcag
     1621 gcgatggtct tttgcggcga tgtgtgtttg tcctctgttg atttattgga accgcccggc
     1681 gaaccagcac cgggcggcat cgtacaggcc aagatgataa catagaggga ttgatccgtc
     1741 aacacgccga tcgatccgcc attgcgagcg gagcgaagca atctcaatat cgattaagtt
     1801 gatctaaacc gatcaggaga ttgctccgtc gcccttcggc ctccacgcaa tgacggttta
     1861 ttctttgctc tcgatcgcct tctgagccat gccgacgaac tccgccagcg gcttggcttt
     1921 gaggtcctcg cctgatcgca agcggatggc aaccgcgtcc gcttcctgtt ccttatcgcc
     1981 gacgacgagc atgtacggaa tcttaagcag ttgtgcattg cggatcttgg cctgcatgcg
     2041 atccgttgac tcatcgactt cggatcgaat
//
LOCUS       DASRTJ010000311         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_56331,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000311 DASRTJ010000000
VERSION     DASRTJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56331"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFF70_06340"
     CDS             complement(<1..188)
                     /locus_tag="VFF70_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HZY44355.1"
                     /translation="MAESVYKVITLVGASTESWEKAANAAVAQAAKSLRDLRIAHVVD
                     QDIQIEDGKPNMYRVKLEV"
     gene            complement(229..1032)
                     /locus_tag="VFF70_06345"
     CDS             complement(229..1032)
                     /locus_tag="VFF70_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44356.1"
                     /translation="MRNYSIALLSAVAAIFLTACGSTSTETVTATIAPTTAGPTKQAT
                     ATKQPTSTSRPTSTPRPPATLKPTKTATPEQTLAPTNAPFSGATISYVDPAGDCIDNN
                     TSKYIICDPLGFDILTATVSRPSETAPLYIIVQLAGQGVADIPRYALMYAFDFDRDAT
                     TGDTKFPPDHGIAPELEIIVDHGIGQAGKLNIYVTQFAADGTKTNGDSTLVEYVVRAD
                     DNLAVVISPDLVPGNQFNMSADMIGSAMFDHIVDHGSLQFQEGTTALVK"
     gene            1273..1542
                     /locus_tag="VFF70_06350"
     CDS             1273..1542
                     /locus_tag="VFF70_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44357.1"
                     /translation="MAQRFEYKVVYADLRGRVSVEGDETLIQDGERMTAFGRRYLNAL
                     GEQGWELVGVQHQPMGAAFHMFKRTLAEGQKAEAAKPIKSDTAPV"
     gene            1761..>2144
                     /locus_tag="VFF70_06355"
     CDS             1761..>2144
                     /locus_tag="VFF70_06355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY44358.1"
                     /translation="MASLNGKVVLITGASCGLGRALALEFARQGAKVVINSRSKGADD
                     LAATERDAKALGAEVLSVLADVSQRADVERLTGEALDHFGRVDVLINNASALGPTPMP
                     YLVDYPIEDFEWVLRTNVTGPFMLTR"
BASE COUNT          449 a          547 c          627 g          521 t
ORIGIN      
        1 acttcgagct tgacgcggta catgttcggt ttaccatcct cgatctgaat atcctgatcg
       61 acgacatgcg cgatgcggag atcgcgcagc gatttggccg cctgcgcaac cgccgcgttg
      121 gccgctttct cccacgactc ggtgctggct ccgactaacg tgatgacttt atacacactc
      181 tctgccattt tttgtctcct ttcaaccgtt caaattctga atagaggctc acttaaccag
      241 agccgtcgta ccttcctgaa attgcagact gccgtgatcg acaatgtgat cgaacatcgc
      301 cgaaccgatc atatcggcgg acatgttgaa ttgattgccc ggcacgaggt caggtgaaat
      361 gactacggcc aggttgtcgt cagcccggac aacatattct accaacgtgc tatcgccatt
      421 agttttggtg ccatcggcgg cgaactgggt aacgtagata ttgagtttac cggcctggcc
      481 gatgccgtga tcgacgatga tttccagttc gggtgcgata ccgtgatcgg gcggaaactt
      541 cgtatcgccg gtcgtggcat ctcgatcgaa atcaaaagcg tacatcagcg catagcgcgg
      601 gatgtccgcg acaccttgcc cggccagctg cacaatgata tagaggggag cagtttcgct
      661 tggccgtgag accgtcgcgg taagaatatc aaagcccagt ggatcgcaga tgatatattt
      721 gctggtgttg ttgtcgatgc aatcaccggc cggatcaacg tagctgattg ttgccccgct
      781 aaatggcgca ttggtcggcg cgagtgtttg ttcaggcgtc gccgttttag tcggcttcag
      841 cgttgcaggc ggtcgcggag tggatgttgg cctcgacgtc gaggtgggct gtttagtcgc
      901 agtcgcttgt ttcgtcggcc cggctgttgt tggagcgatc gtcgcggtta ccgtctccgt
      961 tgatgtcgac ccgcaggctg ttaagaagat ggcagccaca gccgacagca gcgcaatgct
     1021 atagtttctc atctttttct cctcccgtgt gataactgta ccttggtttg tcaatcagtg
     1081 taacgcgaat agccgatcaa agtcaagtgc tttcggcagt gggtatcttg gagaatgatc
     1141 tcctgtctat aatctgtcgg ttagcctgat cgatcgggca gtgtggatcg atcggattca
     1201 gagtatgcta ccatcatcga tgcgtttgat cgatcagcgc atcactcaat ccgatcgaat
     1261 aggagacaat taatggcaca acgatttgaa tataaagtag tgtatgccga tctgcgcggg
     1321 cgcgtgtcgg tggaaggcga tgaaacgttg attcaagacg gcgagcgcat gacggcgttt
     1381 ggccgccgat atctgaacgc gctcggcgag cagggctggg aactggtcgg cgttcagcat
     1441 cagccgatgg gcgcggcgtt ccatatgttc aaacgcacat tggccgaagg ccagaaggca
     1501 gaagcggcca agccgatcaa gtcggacacg gccccggtct aatttggcgc gcgttctaaa
     1561 ccgacacgtg cggtacccgt gcggtaaagc ccgcagggcg taccgaagcg cgcagtgcgt
     1621 accgtcggag cgagcgatct gcttgctccg accgattttg ttttgtccgc ttgattcggc
     1681 ttacttcttc gccaagatcg cgtatactct gagtactgac cagtggctaa gcggctgggc
     1741 taaccggagg agacaagacg gtggcatcat taaatggaaa agtcgttttg attacgggtg
     1801 cgtcgtgtgg attgggacgc gcgttagcgt tggagtttgc acgtcagggc gcgaaagtgg
     1861 tcatcaactc gcgatcgaag ggcgccgatg atcttgctgc aaccgaacgc gatgccaaag
     1921 cgcttggggc agaagtctta agcgttctcg cagatgtttc ccaacgcgcc gatgtcgaac
     1981 gcttaaccgg cgaagcgtta gatcatttcg gccgcgtcga tgtgttgatc aacaacgctt
     2041 cggccttagg tccaacgcca atgccatact tggtggatta tccgattgaa gattttgaat
     2101 gggtgctgcg cactaacgtg accggcccgt ttatgctgac ccgc
//
LOCUS       DASRTJ010000312         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321060,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000312 DASRTJ010000000
VERSION     DASRTJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321060"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFF70_06360"
     CDS             complement(<1..839)
                     /locus_tag="VFF70_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44359.1"
                     /translation="MSNRTAKPLSNLYALAFRNLWTRKTRTFLTALGIILGVAVILAI
                     NIANNSTLASINKIFDEASGTADLVIVPSSFTGQGYSQSISNRVRAIDGVQSAVPSIQ
                     ASTILAADAKGWAFNFALGGSSGNSLLLFGIDPELDTLARQYKLIAGRFLNPGERTYS
                     AIFTEEYAVKKHIEVGKDVEILLPTGTATLRCVGIVRKDGPGLMNSGVVAFVPLSMVQ
                     DLYARQGEIDQIDVVVRTDVGNSTEKLAALKHALQADLGQKFLVNFPAQRGQIVSQQL
                     ATY"
     gene            complement(885..1646)
                     /locus_tag="VFF70_06365"
     CDS             complement(885..1646)
                     /locus_tag="VFF70_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44360.1"
                     /translation="MTILQASHVTKEYRMGEVSVTALDRVEFKVESGEFVAIMGPSGS
                     GKSTLLHLLGGLDGASDGEVTLAGKALSKLNDNEVTMVRRRKVGFIFQFYNLVPTLTA
                     EENITLPLLIDGRNLAEYRDQINQLLELVGLTDRRGHKPDQLSGGQQQRVAIARAFVT
                     DPDIVLADEPTGNLDSKSGKAILELLRHSCDELHHTIVMVTHDAYAASYADRVVFLKD
                     GRTATQLKKSDLHDRDFMSAIMDVMGHLESMTSEE"
     gene            2102..2371
                     /gene="infA"
                     /locus_tag="VFF70_06370"
     CDS             2102..2371
                     /gene="infA"
                     /locus_tag="VFF70_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZY44361.1"
                     /translation="MAKHDEEAKVKEGKVEVEGVVIEALPNTMFTVKLENGHTVMGYL
                     SGKMRRYYIRILLGDRVKVEMSPYDLSKGRITYRFKSGGGPAPEF"
     gene            complement(2368..>3303)
                     /locus_tag="VFF70_06375"
     CDS             complement(2368..>3303)
                     /locus_tag="VFF70_06375"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZY44362.1"
                     /translation="VSEVMGRYAPNGVAIEQLALDINGADEGDAAQLESTVAVRAYLL
                     IDAELEIKKRQIEEGLWHLSHIKPIAAPVFRTVSESDWANAWKEHYHVAHLGDRFVIK
                     PSWREYDAQPHEIVIELDPGMAFGTGLHPTTRMCLAAIEKYLQRRTMLTNRVPAVGID
                     RGDAVDEQSLKKMSVLDLGTGSGILAIGAAKLGAGSILAVDIDPLSVKVATENAIANG
                     VDRIIEVRSGSLAEAAKNDATYDLIAVNILAKIIIQLCEENLGRVVRSGGRAIFAGLI
                     DTQELGVREALQSQSLIVIDRLQDKDWVCLIAQKN"
BASE COUNT          726 a          919 c          885 g          773 t
ORIGIN      
        1 tgataggtag cgagttgctg cgaaacgatc tggccgcgct gcgccggaaa gttcactaaa
       61 aatttttggc cgagatcggc ttgcagcgcg tgtttgagtg cggctaactt ttcggtcgag
      121 ttgccaacgt ctgtgcgcac caccacatcg atctgatcga tctcgccctg gcgcgcatat
      181 aaatcttgca ccatgctgag cggcacaaac gcgacaacgc cgctgttcat caagcccggc
      241 ccgtctttac gcacgatgcc gacgcagcga agtgtagctg tgcccgtggg taaaaggatc
      301 tcaacgtctt tgccgacttc aatgtgtttc ttgacggcgt attcttccgt gaatatagcg
      361 gagtaagttc gctcacccgg attcaagaag cgtccggcga tcaatttgta ttgccgtgcg
      421 agcgtgtcca gttccggatc gatgccgaac aacaacaagc tattgccgct cgatccgccg
      481 agcgcaaaat tgaacgccca gcctttcgca tcggcggcga ggatggtgct ggcttgaatg
      541 ctcggcacgg cggattgcac gccatcgatc gcgcgcacgc gattgctgat cgattggctg
      601 taaccttggc cggtgaaact cgacggcacg atcacgagat cggccgtgcc ggaggcttca
      661 tcgaaaattt tgttgatcga agcgagcgtg ctgttgttgg caatattgat cgccaggatg
      721 acagcgacgc ccaagataat gcccagggcc gtcaagaagg ttcgtgtctt gcgcgtccat
      781 aaatttcgga aggcaagcgc gtataagttg gagagaggtt tagcggtgcg gttgctcatt
      841 gtaatcgata cgtgatgcgt gaaggacttt tcacgtaaca cttttcactc ttcactcgtc
      901 atactttcca aatgtcccat cacatccatg atggccgaca tgaaatctcg atcgtggagg
      961 tcggactttt tgagctgcgt cgccgtgcgg ccatctttca ggaataccac tcgatccgca
     1021 taagaggcgg cgtaggcatc atgcgtaacc atcacgatcg tgtgatgcag ttcatcgcac
     1081 gaatggcgca gcaattccaa gatcgctttg ccgcttttag agtcgaggtt gccggtcggc
     1141 tcgtcggcca gcacgatatc cggatcggtg acgaacgcgc gggcgatggc gacgcgctgc
     1201 tgttggccgc ccgataactg atcgggttta tggccgcgcc gatcggttaa gccgaccagc
     1261 tcgagcagtt gattgatctg atcgcgatac tctgcgagat tgcggccatc gatcaataag
     1321 ggcagggtga tattttcttc agcggtcaaa gtcggaacga gattgtagaa ttggaagatg
     1381 aagcccactt tgcggcggcg caccatcgtc acttcgttgt cgttcaactt cgagagcgct
     1441 ttgcctgcca gcgtgacttc accgtcgctc gctccatcga ggccacccaa tagatgcagc
     1501 agtgtcgatt tgcccgaacc ggacgggccc atgatggcga caaactcacc cgactcaacc
     1561 ttgaactcga cccgatcgag cgcggtgacg ctgacttcac ccatgcgata ttctttggtg
     1621 acgtggctgg cttgaagaat ggtcatagtg ttcctcactt aaccgtcatt gcgagccgcc
     1681 aaccctgcac acttcaggca gggccttcgg ggcggcgtgg caatctcacg atcgaccgaa
     1741 ggtgagattg cttcgtcggc caaacggctt ccttgcaatg acgttcataa aaaagaaatg
     1801 cgcccaatga cttcatgggc gcgtcgtgat gttaccacat cctgttaaga tcgcaagaga
     1861 ttggaaaatc aatcgagtat tcgggggtga tctaaaggaa cttatcaatc ccggctctat
     1921 cgcaatcgac atcgtcaatt tgacaccaac tgcccatctc aggtatactc atcctcgctg
     1981 gacgcctgcg acattcatcc aatcggggat cgaactcgac gtgacacttt gaatctcggc
     2041 agtgacatcg atcgtttaag gcgcaggcag tgcgcctttt tattttccac aaggagccgc
     2101 tatggcgaaa cacgacgaag aagcaaaagt caaagaaggc aaagtggaag tggaaggcgt
     2161 cgtcatagag gcgctgccta ataccatgtt caccgtaaag ctggagaacg gccacacggt
     2221 catgggctat ttgtcgggca agatgcgccg ctattacatc cgcattctgt tgggcgatcg
     2281 tgtgaaggtg gaaatgtcgc cgtacgatct gagcaaaggc cgcatcacct atcgtttcaa
     2341 gagcggcggc ggacctgcgc ccgagtttta attcttctgc gcgatcaagc acacccaatc
     2401 tttgtcttga agtcgatcga tcacgatcaa actctgcgat tgtaacgctt cgcgtactcc
     2461 aagttcctgc gtatcgatca agccagcaaa gatcgcccgt ccgcccgatc gaaccacgcg
     2521 ccccaaattt tcttcgcaca actgaatgat gatcttggca aggatgttta cggcgatcaa
     2581 atcgtaagtt gcatcatttt tcgcggcctc tgctagcgaa cccgatcgga cttcgatgat
     2641 ccgatcgacg ccgttggcga ttgcattttc agttgccacc ttaaccgata gcggatcaat
     2701 gtccaccgcc aggatcgatc ctgcgcccaa cttcgctgca ccgatcgcca ggatgcccga
     2761 tccggtccca agatcaagca cgctcatctt tttgagtgat tgctcatcga ccgcgtcccc
     2821 gcggtcgatt ccaaccgcgg ggacgcggtt ggtgagcatt gttcttcgct gcaaatattt
     2881 ttcgatcgcg gccaaacaca ttcgcgtggt cggatgcagg cccgtgccga aggccatgcc
     2941 cggatcgagt tcgatcacga tctcgtgggg ctgggcatca tactcgcgcc acgatggctt
     3001 gatcacgaat cgatcgccca ggtgcgcgac gtggtaatgt tccttccagg catttgccca
     3061 atcgctttca ctcaccgtgc ggaacactgg cgcggcgatt ggcttgatgt gacttaaatg
     3121 ccacagccct tcttcaatct gccgcttctt gatttccagt tccgcgtcaa tcaacagata
     3181 agcgcgcacg gcaacagtcg attctaattg tgccgcatcg ccttcatcag cgccattgat
     3241 gtcgagtgcc agctgctcga tcgctacgcc gttcggtgcg tagcggccca tcacttcgga
     3301 cac
//
LOCUS       DASRTJ010000313         4530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_245398,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000313 DASRTJ010000000
VERSION     DASRTJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245398"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..560)
                     /locus_tag="VFF70_06380"
     CDS             complement(228..560)
                     /locus_tag="VFF70_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44363.1"
                     /translation="MTDVQPFIEASAALIVALRSGGGMRVKIVEALQWGLPIISTTIG
                     CEGIDVTDQQDILIADEPAAFAGAVITMLTDNDLAQRLADQGRQLVARRYNWRKVYSL
                     LDAIYESI"
     gene            complement(561..1484)
                     /locus_tag="VFF70_06385"
     CDS             complement(561..1484)
                     /locus_tag="VFF70_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44364.1"
                     /translation="MRILFLTQVLPYPLDSGAKVRAYHVLQYLTSRHQVTLVSFSRDS
                     DSAAAIDHLRELCHAVHLVPLKRSRLNEVLDIVRSLVAAQPFTILRDRRRAMHQLLIH
                     LVTDQYFDIIHADQLTMAQYALLADSVILDRSPSARPKLVLDAHNAYYLIPQRMASVS
                     RSFFLKKFLQREARLLAQYEAETYRCFDEVLTVTENDQTSIQQLSPFDSAQPRFTTMP
                     ICVDADQPFVQRRSNPHGLLMLGGLHWPPNADAVRWFAHEVLPVIKQQIPDVQLFIVG
                     ARPPQNILALADVAHLHQLDQANGASIVVTG"
     gene            complement(1491..2405)
                     /locus_tag="VFF70_06390"
     CDS             complement(1491..2405)
                     /locus_tag="VFF70_06390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44365.1"
                     /translation="MTVSIVIVNWNACDVLLRCLRSIEAAAPDRSTDTMVIDNASQDN
                     SVAQIRQQFKWVTLIENAENVGFAAANNQGIRASCGEFILLLNPDTELHSDSLQHLIQ
                     FMRDHPHCGAAGPRLLKPDGTLQPSAFPLPSLGREVWRLFQLDAIKPYALYSMSRWPI
                     DTARSVESVQGACVIVRRAALDQIGLFDEDYFIYSEEIDLCHRLRKVGWSIDWVPQSK
                     VMHYGGQSTRQVAAEMFKWLYQGKITFFRKHSGRPAAVAYKFILGAASLARLLMTPFA
                     WLEPPSQRERHLALARNYWRLIRSLPSL"
     gene            complement(2405..3808)
                     /locus_tag="VFF70_06395"
     CDS             complement(2405..3808)
                     /locus_tag="VFF70_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY44366.1"
                     /translation="MSALTRIFNRSLDEHVVMRWAVIAVILLLSLIFGLFTTRYLALF
                     DLGLAAAIFLFANIELGLVLMLIVSLSLPISIATNTQAVVPISMIVLVLLLIGWLFRM
                     LIRRSFRLAPSRVNLPLGIFTLAATISFVGGGLPWILLAAHAPLTAQLGGWAIFVLSC
                     MGCLLAGNQIKQLKWLKVLVTVFLSYGVLAVVGRLTPAVGALANQLILFTDAGSLFWL
                     WIVALAGGQAFFNKSMQLYGRVALGALALLTVLVIYLTSQSWLSGWLPGLGVLLALLV
                     LRSWRISVLVMGVALALFLVLKGSALAGLVSENQYSIDTRLAAMQILLGRVLPANPLI
                     GLGFANYYHYAELYPILGWYVKFNSHNNYVDIVMQMGVIGFVAFAWLATALTRLGLQL
                     RSRVGDGFARGYVFACIAGLAGMLLAGALGDWFLPFVYNIGLSGTRMSILGWIFLGGL
                     ISIEQIAKRQSETVTGD"
     gene            complement(3810..>4530)
                     /locus_tag="VFF70_06400"
     CDS             complement(3810..>4530)
                     /locus_tag="VFF70_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZY44367.1"
                     /translation="WMMRLLLSIPHYRSSGNFTGQTARYLRIKVGTVVHGHEKYRPEF
                     AELLQSANFRVIVMLRDPRDQTVSRLYHIRRDVGHPFHAQLTVLNQEAALMTLIEGRP
                     GLPGVRSAMAISSSWLDAGADMLCVRYEDLSQDPTRELTKVMQYLAIPADNRLTQAII
                     RRNRFERQTIGRRIWQRPRRQGEIDPASHFRKGTMHDWQNFFTPTHVDCFKAIAGQLL
                     IDLGYEADLNWSAVTPPSESR"
BASE COUNT         1226 a         1193 c         1193 g          918 t
ORIGIN      
        1 cccatgatga gtaccgccaa caggcgtgca gccaccgaga cgatcaagat gattatcaag
       61 gtgtggcgag ttgtgagacg gagcgatcga aggcgggtta gcatggtgga cttactcact
      121 catccgacgc acttcagccg tcgttgcgag gagggctttt tgcgacgtag caatctccgc
      181 catagtcaac gagattgctt cgtcgcaatg acggtgcgta acgtgagtta aatagattca
      241 tatatcgcat cgagcaacga ataaactttg cgccagttgt agcgccgcgc cacgagctgc
      301 cggccttgat cagctaaccg ctgcgcaaga tcattatcag tcaacattgt gatcaccgcc
      361 ccggcaaaag cggctggctc atcggcgatc aggatatcct gctgatcggt cacgtcgatg
      421 ccttcacagc cgatcgtggt cgagatgatg ggcagtcccc attgtaaagc ttcgacgatc
      481 ttcacgcgca tgccgccgcc cgatcgcaat gcgacgatta acgcagcact ggcttcgatg
      541 aagggttgaa catccgtcac ctagccagtc acgacgatcg aggcaccgtt ggcttgatcg
      601 agctggtgaa gatgagcgac atcagctaga gccagaatat tttgaggcgg acgcgctccg
      661 acaataaata gttggacatc tgggatttgc tgcttgatca ccggcaggac ttcatgcgcg
      721 aaccaacgca cggcgtcagc attcggcggc cagtgcagtc caccgagcat caataaacca
      781 tgcggattcg atcggcgctg aacgaatggc tgatcggcat caacacagat gggcatcgtc
      841 gtgaagcgcg gctgagctga atcaaatggg cttaactgct ggatgctggt ttgatcattc
      901 tcagtaacgg ttagcacttc gtcaaagcag cgataggtct cggcttcata ttgagcgagc
      961 agtcgcgctt cacgctgcaa gaattttttc aagaagaagc tgcggctcac actcgccatg
     1021 cgctgcggga tcaagtagta agcattgtgc gcatcgagca cgagtttcgg tcgagcgctg
     1081 ggagatcgat cgaggataac agaatcagcc agcagcgcat attgtgccat cgtcagctga
     1141 tcggcatgga ttatatcgaa atattgatcg gtaacgagat ggatcaaaag ttggtgcatg
     1201 gcgcggcgtc gatcgcgcag gatggtaaag ggttgcgcgg caacgagact gcgcacaata
     1261 tccaggactt cattcaagcg cgaacgtttt agcgggacaa gatgcactgc atgacacagt
     1321 tcgcgcagat gatcgatcgc cgcggctgaa tcgctgtcac gcgagaagga gacaagcgtg
     1381 acttgatgac gcgacgtgag atattgcaag acgtgatagg cgcgcacttt tgcgccggag
     1441 tcaagcggat agggcagcac ttgcgtgagg aataaaatgc gcacggtcaa tcacaaagac
     1501 ggcaacgatc gaattaagcg ccagtagttg cgcgccaacg ccagatgccg ttcgcgttga
     1561 gaaggtggtt caagccaagc gaagggtgtc atcaataatc gtgcgagtga agccgcgccc
     1621 agaataaatt tgtaagcaac agcggctggt cgacctgaat gttttcggaa gaaagtaatt
     1681 ttgccttgat acaaccactt gaacatctcg gctgcaactt ggcgcgtgct ctgcccgccg
     1741 tagtgcatga cttttgattg tggcacccaa tcgatggacc agccgacttt tcgcaggcgg
     1801 tggcacagat caatctcctc ggagtagatg aagtagtctt catcgaacaa gccaatttga
     1861 tccaacgcgg cgcggcgcac gatcacacac gcgccttgca cgctctcgac agagcgtgca
     1921 gtatcgatcg gccaacgcga catggaatag agggcatacg gtttgatcgc atccaactga
     1981 aataaacgcc acacctcgcg gcccagtgaa ggcaggggaa aggccgaagg ctgaagtgtg
     2041 ccatcgggct tgagcaggcg tgggccggcc gcgccgcaat gtggatgatc acgcatgaat
     2101 tggatcagat gttgcaaaga atcagagtgt aattcagtat cggggttgag cagaagaata
     2161 aattcgccgc agctggctcg aatgccttga ttgtttgccg cggcgaagcc aacattctcg
     2221 gcattttcaa tgagcgtgac ccacttaaac tgttggcgaa tttgcgcgac actgttatcc
     2281 tgcgaggcat tgtcgatcac cattgtgtca gttgatcgat cgggagcagc ggcttcaatc
     2341 gagcgcaggc agcgcagcaa aacatcgcac gcattccagt taacaatgac gattgaaacg
     2401 gtcatcaatc gccagtcact gtttcagatt gacgcttggc gatctgctcg atcgagatca
     2461 agccgcctaa aaagatccaa cccagaatac tcatgcgagt accgcttaga ccaatgttat
     2521 agacaaacgg taggaaccaa tcgcctaaag cgccggccag caacatgccc gctaaaccgg
     2581 caatacaggc aaacacatag ccgcgcgcaa aaccatcccc gacacgcgag cgcagctgca
     2641 gacctaaacg agttaatgca gtcgccagcc aggcaaaagc gacgaacccg atcacgccca
     2701 tttgcatgac aatatccacg tagttgttgt gtgaattgaa tttgacgtac cagcctaaga
     2761 taggatagag ctcggcgtaa tgataataat tggcaaagcc caaaccgatc aacgggttgg
     2821 cgggcaacac ccgacccagc agaatttgca ttgcggctaa acgagtatcg atgctgtatt
     2881 gattctcgct aacgagcccg gccagcgccg agcctttcaa cacaaggaaa agggcaaggg
     2941 ctacccccat gaccagcacg ctaatccgcc aagaacgcag cacgagcaag gcgagcaata
     3001 cgcccaaccc cggcagccaa ccggacaacc atgactggct agtcagatat atgacgagaa
     3061 cagtcagcaa cgctaaagca cccagtgcca cgcggccata cagctgcatt gatttattga
     3121 agaaagcctg cccacccgct aaagcgacaa tccatagcca aaataaactt ccagcatcgg
     3181 tgaataaaat cagctggttt gccaatgcgc ccacagccgg agtcaaccta ccgaccacgg
     3241 cgagaacccc atagctgaga aaaacggtga ccaacacttt taaccatttc aactgcttaa
     3301 tttgattgcc cgctaacaag cagcccatgc aagacaacac aaaaattgcc cagccgccca
     3361 gctgcgctgt cagaggcgca tgggctgcca gtaaaatcca tggcaagccg ccgcccacaa
     3421 atgagatcgt agccgccaag gtaaatatac cgagcggcag attaaccctc gaaggcgcca
     3481 atcggaaact gcggcggatg agcatacgaa acagccagcc gatcagaagt agtaccaaca
     3541 ctatcattga gattggcaca accgcttgtg tgttcgttgc aatactgatc ggcaggctta
     3601 gtgacacgat caacattagg acaagaccaa gttctatatt ggcaaagagg aatatcgcgg
     3661 cagccaaacc caagtcaaac aacgccaggt atcttgttgt gaacagaccg aagatcaggc
     3721 tgagcagcag gataacagcg ataacagccc agcgcataac gacatgctcg tccagtgatc
     3781 gattgaatat tcgtgttaag gcactcatct tatctgcttt cacttggtgg ggtaactgcc
     3841 gaccaattca gatcggcctc atagccaaga tcgattaaga gttggcctgc aatcgctttg
     3901 aagcaatcta catgcgtcgg cgtgaagaaa ttttgccaat catgcatcgt cccttttcga
     3961 aaatgtgaag ccggatcgat ttcgccttgt cggcgaggtc tctgccagat tcgacgtcca
     4021 atcgtttgcc gctcaaatcg gttgcggcgg ataatcgctt gcgtcaaacg gttatccgcc
     4081 gggatggcta gatattgcat gactttggtt agttcacggg ttggatcctg acttaaatct
     4141 tcatagcgaa cacatagcat atcggcgccg gcatccagcc aagatgaact gatcgccatc
     4201 gctgaccgga cgccgggcag cccggggcgt ccctcgataa gagtcatcag cgcagcttct
     4261 tggttcaaga cagtcaattg agcatgaaaa ggatgaccca catcgcgccg aatgtgatac
     4321 aaacgcgata ccgtttgatc gcgcggatcc cgtaacatga cgataactcg aaaattcgcg
     4381 gactgcaaca attcagcaaa ctccggccga tacttttcat ggccatgaac gacagtgccg
     4441 accttgatcc gaagataacg cgcagtttgg ccagtgaaat ttccgctgct ccgataatgt
     4501 ggaatggaca ataataaacg catcatccac
//
LOCUS       DASRTJ010000314         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321160,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000314 DASRTJ010000000
VERSION     DASRTJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321160"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFF70_06405"
     CDS             <1..415
                     /locus_tag="VFF70_06405"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44368.1"
                     /translation="YVDDNIDPDGPTMDVSPGWITWNINSSVTAPQTRTLTVSNFGTD
                     SLTWHVSSDSGWLTVSKATGNDGETLIATGNPAGLKNGETWIGHLIFTTNSNGITQTV
                     PLPVSLIKGDLYQSPYLGPMPPLKFVYLPIVLRQH"
     gene            complement(524..2347)
                     /locus_tag="VFF70_06410"
     CDS             complement(524..2347)
                     /locus_tag="VFF70_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44369.1"
                     /translation="MADDYEFEEEEFTTQFNGQTLRRILAQTRPHWKWVAGFLIAVGL
                     VVLLDSIFTYLRKQMIDQGIVAGDQQALTQIMTIYGLMILFQAVGVFSFIFLAGILGE
                     RVQYDLRQKMFNHLQSLSLTYFSKTPVGWIMSRVTSDSVRISELVTWGLLDSTWGALS
                     IVTSFIFMLAINWQLALIVLLSIPILLFVAVTFKRKIIVEFRNVRRINSKITGAYNES
                     ITGVRVVKALGREDENLREFGELTGEMYHAGFRAAWLSALFLPAVQIISAVAIGTIIW
                     YGGSQVRIAGITIGGLQAFISYVTFMMWPIQDLARVYAELQHAIASAERVFSLIDSVP
                     DVVDRPNPIDPGTLKGDIDFDHVDFTYEAGKAVLIDFSLTVKQGETIALVGPTGGGKS
                     TIVNLICRFYEPVHGSICIGGRDYTELSLHAIQSRIGVVLQTPHLFSGSIRENIRYGR
                     LDATDQEIEDAAKLAGAHDFIITLEKKYAEPVGEGGNLLSVGQKQLISLARAVLAKPE
                     IFIMDEATSSVDTLTEALIQRGMEQLMQGRTSFIIAHRLSTIKRADRILVIENGGISE
                     MGTHAELLRKRGHYYNLYTRQFRHDLSQAYDLFKVAEAELN"
     gene            complement(2442..>2666)
                     /locus_tag="VFF70_06415"
     CDS             complement(2442..>2666)
                     /locus_tag="VFF70_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44370.1"
                     /translation="MKDRTTFIIAHRIQSVMNADLILVMDHGRIVQSGQHEYLIGQPG
                     IYRRIYDIQMRIETELEQEVNQAQAAELNQ"
BASE COUNT          610 a          774 c          693 g          589 t
ORIGIN      
        1 ctatgtggat gacaacatcg atccggatgg gccgacgatg gatgtgtcac ccggctggat
       61 cacgtggaat atcaacagca gtgtgactgc accgcagaca cgcacactga ccgtatcgaa
      121 tttcggcacg gacagtttaa cgtggcacgt cagcagtgat tcaggatggc tgactgtttc
      181 gaaggcaacg ggtaatgacg gcgagacact catcgcgacc gggaatcctg ccgggctgaa
      241 gaatggcgaa acatggatcg gccacctgat cttcacgacg aacagcaacg gtatcactca
      301 aaccgtcccg ttgcccgtat cattgatcaa aggcgatctg tatcaaagtc catatcttgg
      361 cccgatgcca ccactcaaat ttgtatatct gccgatcgta ttacgacaac attaactttc
      421 agatgtcgta tcggttattg ataggcaaga gacccaaagc aaagcccgat cgcttgaaga
      481 cgatcgggct tttgttattt gtttgctgct cggggttatg cccttaattc aactccgcct
      541 ccgccacctt aaacagatca tacgcctgac tcaaatcgtg ccggaactgc cgcgtataca
      601 aattgtagta atgcccgcgc ttccgcagca gttcggcgtg cgtgcccatt tccgaaatgc
      661 cgccgttctc gatcaccaga attcgatcgg cgcgcttgat cgtgctgagc cggtgtgcga
      721 tgatgaaact cgtgcggcct tgcatcaact gctccatgcc gcgttgaatc aacgcctcgg
      781 tcagcgtatc gaccgaactc gtcgcttcgt ccatgatgaa aatttccggc ttcgccagca
      841 ccgcgcgcgc caaactaatc aattgtttct ggcccactga caacaagttg ccgccctcgc
      901 ccaccggttc ggcatacttt ttctccagcg tgatgatgaa atcgtgcgcg cccgccaact
      961 tcgccgcgtc ttcgatttct tgatcggtcg cgtccagtcg tccatagcgg atgttctcgc
     1021 ggatcgatcc gctgaacaag tgcggcgttt gcagcaccac gccgatgcgc gattggatcg
     1081 cgtgcagcga caactcggtg tagtcgcgtc cgccgataca gatcgatccg tgtaccggtt
     1141 cgtagaagcg gcagatcaga ttcacgatcg tgcttttgcc gccgccggtc gggccgacga
     1201 gcgcgatcgt ttcgccctgc ttgaccgtca acgaaaaatc gatcagcacc gctttgccag
     1261 cctcgtacgt gaaatcgacg tgatcaaaat cgatatcgcc tttcagcgtg ccgggatcga
     1321 tcgggtttgg gcgatcaacc acatccggca ctgaatcgat caacgagaag acccgttcgg
     1381 cggaagcgat cgcatgctgc agttcggcat acacgcgcgc caaatcttga atcggccaca
     1441 tcataaatgt gacatatgag atgaaggctt gcagtccgcc gatcgtgatc ccggcgatcc
     1501 ggacttgcga tccaccgtac cagatgatcg tgccgatcgc gaccgcgctg ataatttgca
     1561 cggcgggcag gaacaacgcg gacagccatg cggcacggaa gccggcatga tacatttcgc
     1621 cggtcagttc gccgaattcg cgcaagtttt catcttcgcg acccagcgct ttgacgacgc
     1681 gcacgccggt gatgctctcg ttatacgcgc ccgtgatctt ggaattgatc cggcgcacgt
     1741 tgcgaaactc gacaatgatc ttccgcttga aggtcacggc gacgaataac agaatgggga
     1801 tggacagcaa aacgatcaac gccaattgcc agttaatcgc cagcatgaag atgaaggagg
     1861 tgacgatgct cagcgcgccc cacgtgctgt ccaacaaacc ccacgtcacc aactcggaga
     1921 tgcgcaccga atcggacgtg acgcgcgaca tgatccagcc gaccggcgtc ttgctaaagt
     1981 acgtcagcga caaactctgc aggtgattga acatcttctg acgcagatcg tactgcacgc
     2041 gctcgcccag aatgcccgcg aggaaaatga aactgaacac gcccacagct tgaaagagga
     2101 tcatcaaacc gtagatggtc atgatctgag tcaacgcctg ttgatcaccc gccacgatac
     2161 cttgatcgat catctgtttg cgcaggtagg tgaagatcga atcgagcagc acgaccagcc
     2221 ccacggcgat caggaaaccg gccacccact tccagtgcgg ccgggtctgc gccagaatcc
     2281 gtcggagcgt ctggccgttg aactgcgtcg taaattcttc ttcttcgaat tcgtaatcgt
     2341 ctgccatgtt aaatccattc gtaggggcga gccttgtgct cgcccagggc aaccacgtgg
     2401 gttgccccta cgcatcaatc caattctttt tcattcgtgg atcactgatt caattccgct
     2461 gcttgcgctt gattgacttc ctgctctaac tccgtctcaa tccgcatctg gatgtcgtaa
     2521 atccggcgat agatgccggg ctgcccgatc agatattcgt gctgaccgga ctgaacaatg
     2581 cggccatgat ccatgaccag gatcagatcg gcattcatga cgctttgaat gcgatgcgcg
     2641 atgatgaacg tggtgcgatc tttcat
//
LOCUS       DASRTJ010000315         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_159261,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000315 DASRTJ010000000
VERSION     DASRTJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159261"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..344)
                     /locus_tag="VFF70_06420"
                     /pseudo
     CDS             complement(147..344)
                     /locus_tag="VFF70_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            complement(430..1542)
                     /locus_tag="VFF70_06425"
     CDS             complement(430..1542)
                     /locus_tag="VFF70_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZY44371.1"
                     /translation="MSDYRDLNTTARILLGPGPSMVHPRVLRAMAHPLVGHLDPQFIA
                     LMNEVQELLRFVFQTQNRVTIPVSGTGSAGMEAALCNFIEPGDSVLIGVNGYFGERLC
                     DMAARYGAEVRRLDRPWGEVFTVEEIELALRQKPAKLVALVNAETSTGAWQPMEGMAD
                     LVHHYGGLLVMDCVTSLGGLPVKVEAWGVDVAYSGTQKCLSCPPGLSPLTISPRAVEA
                     LHRRTTQVPNWYLDLTLIEKYWGSERTYHHTAPISMNYALREALRLVYEEGLEARFER
                     HQANARMLWEGLEALGLRLHVPLEYRLPSLTTVLVPDGVDEAVIRRRLLDEYNIEIAG
                     GLGTLKGRVWRIGLMGYSSRPENVLLLLAALERLLK"
     gene            complement(1722..2348)
                     /locus_tag="VFF70_06430"
     CDS             complement(1722..2348)
                     /locus_tag="VFF70_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZY44372.1"
                     /translation="MKTVVCYGDSNTWGFNPATQDRFSIDKRWTGVLAQELGTGYRVI
                     EEGLNGRTTIWDDPIEEGRNGQTYFLPCLWSHQPIDLVTIMLGTNDLKERFALSAYDI
                     AAGAGVLVNLALKSGAGPNAGAPQVLLMSPPVVGRLTDYAELFVEAEARSRKFAGHYA
                     RVAKARRCHFLDTAPSVTSSRLDGIHFEADQHSGLGLAVAARVREILD"
     gene            complement(2377..3171)
                     /locus_tag="VFF70_06435"
     CDS             complement(2377..3171)
                     /locus_tag="VFF70_06435"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY44373.1"
                     /translation="MSDLQGRVVVVTGAGRGIGKTIAHVLAQKGMQVGINDVNVAMIE
                     SVVNEFRAEGLSAIGLQGDVSSKADAAAMIEKAEAELGPLWLLVNNAGVLNAGPTAEL
                     SEAAWDTAMAVDAKGVFLCSQAAIQKMIPRGQGRIVNVASIAGLIVRTGQIAYCSAKA
                     AVIHFSRCLAVEMAPHGITVNCLCPGMTRTQMLSQSAAERGLDLDAMVELIPAGRMAE
                     EKDHAHLIAYFASDESRHVTGQVVTVDGAQSLYHPLMPRVVTRPPT"
     gene            complement(3209..4243)
                     /locus_tag="VFF70_06440"
     CDS             complement(3209..4243)
                     /locus_tag="VFF70_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HZY44374.1"
                     /translation="MKALVLEQYHQLTYKDVPEPQIQPDEVLVQVKACGICGSDVHGI
                     DGSTGRRVPPIIMGHEASGVIVDVGREVRSWQNGDRVTFDCVVHCGQCYFCQRGLINL
                     CDQRRWIGVSLPASRTNGAFAEYVAVPQQVLHRLPDGMPDPRAALMETLSIAFHGVKR
                     VPLALHDSAVVVGAGMIGLLTIQLLKIAGCSQIIAVDSQPARLDLARRFGATLGLRSD
                     IDDVSTIVAQCTNGRGADVGFEVVGLTPTIGMAIASVRKGGALSLIGNFAPTVELPLQ
                     DVVLRQLSLFGSANAVDEQDACLELLASGAVNVDDMISAVAPLSEGAAWFDRLYQREP
                     GLMKVILEPG"
     gene            complement(4271..5080)
                     /locus_tag="VFF70_06445"
     CDS             complement(4271..5080)
                     /locus_tag="VFF70_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZY44375.1"
                     /translation="MKKGLVMCGPDVAYGPLALLSGTFEEKAHKAGALGYDGIELMVR
                     DPAGLDWAAVKATLDAAGVEVPQVVTGELFGADGLCLATSDDDLYHRAETRLRSVIDL
                     AAYLNAMVNIGRVRGRLEWLNKSDRAWEVAVGRLRGVAAYAATKNVRITLEPINRYET
                     DFILSAADGMRLVHDLQQDNFGLMLDVFHMNIEDASIEDGLRMAGDKLWHVHIADSQR
                     RYPGTGHLNFDGVFATLKDMGYRGYVSAELFPWPDPDTAARKTIEFLNRYN"
     gene            complement(5090..5743)
                     /locus_tag="VFF70_06450"
     CDS             complement(5090..5743)
                     /locus_tag="VFF70_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZY44376.1"
                     /translation="MPDRLVLIHTVPPLVDVFKRLCAELLPDVQLYHVLDEPLLERVR
                     QHQGLATDDLAQLQAHIDVAAQIGARAVLVTCSTLSPGVDHITANIPIVKIDEAMIAQ
                     AVGLGRSIGVIATAQSTLEPTCLLLTTAAHSRNKSIEIQTTLVDHALASLLQGDGATH
                     DRLVAQAIAEMSPQVNVVVLAQASMARVIDVRPQPERKIPILTSPHTAIERVRQYLT"
     gene            complement(5760..6392)
                     /locus_tag="VFF70_06455"
     CDS             complement(5760..6392)
                     /locus_tag="VFF70_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZY44377.1"
                     /translation="MSVLNDLRQNVIDGNAAEVEDLVNQAISDGLPPEKIMNEGLISA
                     MTEVGQLFESGEFYVPEMLVAARAMKGGLELLRPQLAAAHIQAIGKVVIGTVQGDLHD
                     IGKNLVGMMLEGAGFEIIDLGADVSPQKFVDAVQEHQPNFVGCSALLTTTMPKMKATI
                     EALKEAGLRDRVKVMIGGAPITAQYAAEIGADIFAPDAASAATRAKQALQ"
     gene            complement(6418..7572)
                     /locus_tag="VFF70_06460"
     CDS             complement(6418..7572)
                     /locus_tag="VFF70_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZY44378.1"
                     /translation="MAEMTSRERVWTTLNHQEPDRVPLDIGGGASTTIVVEGYEKFKQ
                     KLGVSSEIRIMNKAFRLARLDEEVMKRLGSDCRPVGIKGPAHWTPPPSEPGTFIDVWG
                     ITWRQAFYGDNCYYWEVARNPLAEASIEDLDRYPWPDPLDPGYTAGLADEVKTLYEDT
                     DYALMADGGFKSFWELGYMLRGYQQMLMDLVGNPNFVSALMAKLLVINIAGTGRFLDI
                     AGPYIHVFRAADDLATQKGLLMSPKTYRTLLKPFYKKYFDFVKSKTEAKIFYHSCGNV
                     AGLIDDLAEIGVEILNPVQVSALGDTAALKARYGDKVTFWGGIDTQHVLPNGTVAEVE
                     AEVRTRIRDLGPRGGFVVGSVHNIQPDVPPQNILAMAEATRKFGTYPLAI"
     gene            complement(7591..>8476)
                     /locus_tag="VFF70_06465"
     CDS             complement(7591..>8476)
                     /locus_tag="VFF70_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZY44379.1"
                     /translation="PVFSNARITELRPKVAAIAAGLVDRLIAQDGGDLVTDYAIPLSV
                     GTLAEFTGLPRADSGKWVAWIRQMFDVYDRAGAAQASAEFGRYIDELIAAHRREPCGD
                     FISMLIESEVDGHRLTDKELHSFCTVLFGAGFETTADAMSVMLYYLAERPVEYQQLAA
                     QPQLISSAVEEFLRYISPIQIFGRNATQQIELHGQTIRPRDVVALSFGSANHDPKVFS
                     DPEWCVLDRTPNPHVAFGAGIHLCLGAPVARLEMQVTLEEFTRRVPEYHLAPQTIVAW
                     KTRGDRRGLAALPVVLTS"
BASE COUNT         1845 a         2656 c         2226 g         1749 t
ORIGIN      
        1 gatgtagccg ctgccgactt tgcgcatgaa gatgattgcc aattgcgctg ccctgttgaa
       61 cccaaggcga agccgaaggc gcgtcgggtg catgctatgg gccgcatttc actgcagtcc
      121 ccggcacctt acttcttcat agaactgagg taatcttcgg gatcttgcaa ggcccaatcg
      181 ggtcccaggc gaatcttggt gcctggcaga ttttcgtcga tgacacggtt ggaatatgga
      241 taaacagctg tggctagttc ttggccgttg ctcacatcca ccagcaccgc gcggcctgat
      301 tccgtgccga agtcaacccc gatagcatat tcgcgtttag ccatgttgcc ctcctattga
      361 tccagttttc agattcaagt gatttcaaga atagctgggc agcattatca agatgcgcgt
      421 gagctagctc tatttgagta accgctccaa ggccgccagc agcaggagaa cgttctctgg
      481 ccgcgatgaa tagcccatca agccaatacg ccacacgcgc cccttcaaag tgcccaggcc
      541 accggcgatt tcaatgttat attcatccag caatcgtcgt cgaatcactg cttcatctac
      601 gccatcgggc acgagcaccg ttgtcaacga aggcaaacga tactccaaag gcacgtgcaa
      661 ccgcagaccg agcgcttcca gtccctccca caacattcgc gcattggctt gatgacgctc
      721 aaagcgtgcc tcaagaccct cttcataaac caggcgaagt gcttcgcgca aagcgtagtt
      781 catgctaatg ggcgccgtat gatgatatgt gcgctcgctg ccccaatatt tttcaatcag
      841 cgtcaaatcg agataccaat taggcacctg ggtcgttctt cggtgcagag cttcaacggc
      901 gcgcgggctg atcgtcagcg gcgacaagcc cggcggacaa ctcaagcatt tctgcgttcc
      961 actataagcg acatcgacac cccaagcctc gactttcacg ggcaagccac ctaacgaagt
     1021 gacgcaatcc atcaccaaca gcccgccgta atgatgcacg aggtcggcca taccctccat
     1081 cggttgccaa gcaccggttg aggtctcggc attcaccaga gccaccagtt tggcgggttt
     1141 ctgccgcagg gccagctcaa tctcctcgac cgtaaatact tcaccccacg ggcgatcgag
     1201 gcggcgcacc tcggctccgt agcgcgcggc catatcacac aggcgctcgc caaaatatcc
     1261 gttcacgcca atcagcaccg agtcgcccgg ttcgatgaaa ttgcacaggg cggcctccat
     1321 ccccgcgctg cctgtcccgg agaccggtat cgtcacgcga ttttgcgtct gaaacacgaa
     1381 ccgcaataat tcctgcactt cattcatgag cgcaataaac tgcggatcga gatggccgac
     1441 gagcggatgg gccatcgcgc gcaagacgcg cggatgcacc atactcggtc ccggccccaa
     1501 cagaattcga gcagtcgtat tgagatctcg atagtcactc attcgtttct cctgatatgt
     1561 ttgaattgat tcagcgccag cctggataaa ccggcgcgtg ttagaagaca gcacagtggc
     1621 gagcttgcgc cgacggtccg cacacaacaa ccttgtggct cgccaaggat aaacacggac
     1681 gtcggcggct gtattatcca atgggatgga cagagttcag ctcagtcgag aatctcacgc
     1741 acgcgagccg cgaccgccag gccgagccca gagtgttggt ctgcctcaaa atggattcca
     1801 tccagccttg atgacgtgac gctgggcgcc gtgtccagaa aatgacaccg gcgggcttta
     1861 gcgacgcgcg cataatggcc ggcaaacttg cgtgatctag cttcggcttc gacaaacagc
     1921 tcggcgtagt cggtgagtcg gcccaccacc ggcggagaca tcagcaagac ctgcggcgcc
     1981 ccggcgtttg gcccggcacc acttttcagc gcaaggttca ccagtacgcc cgcgcccgcg
     2041 gcgatgtcat aagccgacag cgcaaagcgt tctttcaagt cattcgtacc aagcatgatc
     2101 gtgaccagat cgatcggctg gtgtgaccac agacacggca gaaaataggt ctggccattc
     2161 cgcccttcct caatcggatc atcccagata gtggttcggc cattcaaccc ctcctcgatg
     2221 acccggtagc cggtgccgag ttcctgagcc agcacgccgg tccaacgctt atcgatcgag
     2281 aagcgatcct gcgtagccgg attgaagccc cacgtatttg aatcaccgta acagacgacg
     2341 gttttcatgc ttcctcctcg aaatccgagt gcgcaactac gttggcggcc gggtgaccac
     2401 tctgggcatc aacggatgat aaaggctctg cgcgccgtcc accgtcacca cctgtccggt
     2461 cacgtgcctg gattcgtcgg acgcaaagta agcgatgaga tgagcgtgat ctttttcttc
     2521 cgccatgcgg cccgccggga tcagttccac catcgcatcc agatcaagcc cgcgttcggc
     2581 cgccgactgg ctcaacatct gggtacgggt catgccggga cacagacaat tgaccgtgat
     2641 gccatgcggc gccatctcca cggcgaggca gcgtgaaaag tgaatcaccg ccgccttcgc
     2701 cgagcaatag gcaatctgcc ccgtgcgcac gatcaggccg gcgatggaag cgacgttgac
     2761 aatgcgccct tgcccgcgcg gaatcatttt ttgaatcgcc gcctgcgaac aaagaaagac
     2821 ccctttggcg tccacagcca ttgccgtgtc ccacgccgct tcacttaact ccgccgtcgg
     2881 gccggcgttc aatacacccg cattattcac caacaaccac aacggcccca gttccgcttc
     2941 ggccttttcg atcatggcgg cagcatctgc tttggaagac acatcgccct ggaggccaat
     3001 ggccgacaag ccctccgcgc gaaattcatt gacgacactt tcaatcatcg ccacattcac
     3061 atcgttgatg ccgacctgca tgcccttttg ggccagcacg tgggcgatcg ttttaccaat
     3121 gccccgaccg gctcccgtga ctacgacgac tctgccttgt aagtctgaca tgtgttgctc
     3181 cttttgattt aaagtcttag tgtgtcaatc agccaggttc aagaatgacc ttcatcaaac
     3241 ccggctcacg ttggtacaac cgatcgaacc aggctgcccc ttcggacaag ggcgcgacag
     3301 cgctgatcat atcgtctaca ttcacggcgc cggatgctag cagttccaga caggcatcct
     3361 gttcgtcgac ggcattggcc gagccaaaca aagacaattg tcgcaacact acatcctgca
     3421 acggaagttc aactgtcggc gcaaaatttc cgatcaaact caatgccccg cccttgcgca
     3481 cggaagcgat ggccatcccg atcgtcggag tcagaccgac aacttcaaaa ccgacatcgg
     3541 ccccgcgccc gttcgtgcac tgcgcaacaa tcgttgacac atcgtcgata tccgaacgca
     3601 gcccaagcgt cgcgccgaag cggcgggcca gatccaaccg cgccggctgc gaatcgacgg
     3661 cgatgatctg cgagcagccg gcgatcttca acagttgaat cgtcagcaaa ccgatcatgc
     3721 ccgcgcctac tacgacggcg ctatcatgca gcgccaacgg cacgcgcttg acgccgtgaa
     3781 acgcaatcga caaagtttcc atcagcgccg ctcgcggatc aggcataccg tccggcaaac
     3841 gatgcagcac ttgctgcgga acggctacat attcagcaaa ggcgccgttc gtgcgagatg
     3901 ccggcagcga aacgccgatc caacgccgtt gatcacacag gttgatcaag ccgcgctggc
     3961 aaaaatagca ctgaccgcaa tgcaccacgc agtcaaacgt cacccgatcg ccgttctgcc
     4021 aacttcgcac ctcccgcccg acatcgacga tcacgccgga cgcttcatgc cccataatga
     4081 tcggcggtac acgccggccg gtgctgccat cgatcccgtg gacgtcgctg ccacaaatgc
     4141 cgcaggcttt gacttgaacc aagacttcat ccggctgaat ctgtggttca ggaacatctt
     4201 tgtaagtcag ttgatggtat tgctctaaga ccaaggcttt cattgcataa cccgacttgt
     4261 cgtcccgcgc tcagttgtat cgattgagaa attcgatcgt cttgcgtgca gccgtatcag
     4321 gatcaggcca tggaaatagt tcggcggaaa cgtagccccg atagcccata tctttcagag
     4381 tggcaaagac accgtcaaaa ttcaagtggc ctgtgcccgg atagcggcgc tgcgaatcgg
     4441 caatgtgcac atgccacaat ttgtcgccgg ccatacgcaa gccgtcttcg atcgaagcgt
     4501 cttcgatgtt catgtgaaat acgtcgagca tcagaccgaa gttgtcttgc tgtagatcgt
     4561 gaaccaaacg catcccgtcg gcggcgctca ggatgaaatc cgtctcatag cgattgatgg
     4621 gctccaacgt gatgcggaca tttttcgtgg ccgcataagc ggccacaccg cgcaaccgac
     4681 cgaccgccac ctcccatgcg cgatccgatt tgttcagcca ttccagtcga ccgcgcactc
     4741 gcccgatgtt caccatcgcg ttcaagtaag ccgccagatc gatgactgat cgcagccgcg
     4801 tttcggcgcg gtggtagaga tcatcatccg aggtcgcaag gcacagcccg tcggccccga
     4861 acaattcgcc ggtaaccacc tgtggtactt ccacccccgc ggcatcgagt gtcgctttca
     4921 cggcggccca atccaaaccg gccggatcgc gtaccatcag ctcgatgccg tcataaccca
     4981 aggcccctgc cttgtgcgcc ttctcttcga acgtgccgct tagcaaggcc aacgggccat
     5041 aggccacatc cggcccacac atcacgagtc cttttttcat tgcctttcct catgtcaaat
     5101 actgtcgcac acgctctatg gccgtgtgcg gactagtcaa gatcggaatt ttgcgttcgg
     5161 gttgcggtcg cacgtcgatg acacgcgcca tagacgcttg cgccaacacc acaacattca
     5221 cctgcggaga catttcagcg atcgcctggg ccaccaaccg atcatgcgtt gcgccatccc
     5281 cttgcaacaa tgacgccagc gcatgatcaa ccaaagtcgt ctgaatctcg atcgatttat
     5341 tgcgactgtg cgccgccgtc gtcaatagca agcacgtcgg ttccagtgtc gattgcgcgg
     5401 tggcaattac gccgatcgat cgacccaacc cgacggcctg tgcgatcatc gcttcgtcga
     5461 tcttgacgat cgggatattg gcggtgatgt gatcgacgcc cggcgacaag gtggagcaag
     5521 tcaccagcac cgcgcgcgcg ccgatctgtg ccgccacgtc gatatgcgcc tgcagctgcg
     5581 caaggtcatc ggtagccaat ccttggtgct ggcgcacgcg ttctagcagt ggctcatcca
     5641 gcacgtgata cagctgcacg tcgggcagca gctcggcgca cagtcgtttg aacacatcga
     5701 ctagcggcgg aaccgtatgg atgaggacta aacgatcggg catgagcgga tctttctgct
     5761 cactgtagcg cttgcttggc gcgcgtcgcc gccgacgccg catcaggcgc aaaaatgtcg
     5821 gccccaattt cagctgcata ttgtgccgtg atgggcgcac cgccgatcat caccttgacg
     5881 cggtcgcgca agccagcctc tttcagtgct tcgatcgtcg ctttcatctt tggcatcgtc
     5941 gtcgtcaata gcgctgagca accgacgaaa ttcggctggt gctcttggac ggcatctaca
     6001 aatttttgcg gcgacacatc ggcgcccagg tcgatgattt caaagcccgc gccttccagc
     6061 atcatgccga ccaggttctt gccgatgtcg tgcaagtcgc cttgcacggt gccgatgaca
     6121 actttaccaa tcgcctgtat gtgggcggca gccagctgcg gtcgcaacaa ttcgaggccg
     6181 cctttcatcg cgcgcgccgc aacgagcatt tccggcacat agaactcacc gctctcaaac
     6241 agctgcccga cttccgtcat cgccgagatc agtccttcgt tcataatctt ttccggcggc
     6301 agaccatcac tgatcgcttg gttgaccaaa tcttccactt cggcggcgtt gccgtcgatg
     6361 acattctgcc tgagatcatt caatacgctc atgttttcct ccgagttgtg aaatcgtcta
     6421 gattgccagc gggtaagttc caaacttgcg cgtcgcctca gccatcgcga ggatattttg
     6481 cggcggcaca tcgggctgaa tattgtgaac cgagcccacg acaaagccgc ctcgcggccc
     6541 taagtcacga atgcgcgtgc gcacttcggc ttcgacttct gccaccgtgc cgttgggcag
     6601 gacgtgctgc gtatcgatcc cgccccagaa ggtgactttg tcgccatagc gggccttcag
     6661 ggcggccgta tcacccaagg ccgagacttg cacgggatta agaatctcga cgccgatttc
     6721 ggccaggtca tcgatcaagc cggccacatt gccacagctg tgatagaaga tcttggcttc
     6781 cgtcttggac ttgacgaagt caaaatactt tttatagaag ggcttgagca acgtccgata
     6841 ggtcttgggc gacattaaca atcctttttg cgtcgccaga tcatcggcag cccgaaagac
     6901 gtggatataa gggccggcga tatcgagaaa gcgtcccgtg cccgcgatgt tgatcaccag
     6961 cagcttcgcc atgagcgctg atacaaaatt cggattaccc acgaggtcca tcaacatttg
     7021 ctggtaaccc cgcagcatgt agcccaattc ccaaaagctc ttgaaaccgc catcggccat
     7081 caaagcatag tcggtatctt catacagcgt ttttacttca tcagccagtc cagccgtgta
     7141 gccaggatcc aacggatcgg gccacggata gcgatccaaa tcttcgatgc tcgcttccgc
     7201 cagaggattg cgcgcgactt cccagtaata gcagttgtcg ccgtagaagg cttgccgcca
     7261 ggtgatgccc cacacatcga tgaaggtgcc cggctctgac ggcggcggcg tccagtgcgc
     7321 cgggcctttg atgcccaccg ggcggcaatc gctgcccagg cgcttcatca cctcttcgtc
     7381 cagccgcgca agacgaaagg ctttgttcat gatccggatc tcgctggaga cgcccagctt
     7441 ctgcttgaat ttttcatagc cctccaccac aatcgtcgtg ctggcaccgc cgccgatgtc
     7501 gagcggaact cgatcgggtt cctggtggtt aagggtcgtc catactcttt cgcgcgaagt
     7561 catttcagcc atgatgttct cctttcaggt ttatgaagtc aacacaacgg gcaacgctgc
     7621 cagcccgcgc cgatcgccgc gcgtcttcca cgcgacaatt gtttgcggcg caaggtgata
     7681 ttcggggacg cgtcgcgtaa attcttccag tgtcacctgc atctccagcc gggcgaccgg
     7741 tgcgccgagg cagagatgaa tacctgcgcc aaaggcgacg tgaggattgg gcgttcgatc
     7801 gagcacacac cattccggat cggagaagac cttgggatcg tgattggctg aaccgaaact
     7861 taaagcgacc acatcccttg gcctgatcgt ctgcccatgc aactcgatct gctgcgtggc
     7921 gtttcggcca aagatctgaa tcggcgaaat gtagcgcaga aattcttcga ccgccgatga
     7981 gatcagctgc ggctgcgcgg ccaactgctg atactcgacc gggcgctcgg ccagataata
     8041 gagcatgaca ctcatcgcat cggccgtcgt ctcaaagccg gcgccgaaca aaacggtgca
     8101 gaacgagtgc agttctttgt cggttagacg atgaccatcc acttcagact cgatcagcat
     8161 gctgataaag tcgccacacg gttctcgccg gtgcgcagcg atgagctcgt cgatgtagcg
     8221 cccgaactcg gcgctggcct gggcagcgcc agctcgatcg tacacatcaa acatctgccg
     8281 gatccatgcc acccatttgc cggagtcggc gcgcggcaag ccggtaaatt ctgctaacgt
     8341 tccgaccgaa agcggtatgg cataatcggt gaccagatcg ccgccatcct gcgcgatcag
     8401 ccgatcaacc agcccagcgg caatcgcggc aactttgggg cgcagctccg tgatgcgcgc
     8461 attcgagaag acgggc
//
LOCUS       DASRTJ010000316         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_93686,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000316 DASRTJ010000000
VERSION     DASRTJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93686"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..964)
                     /gene="yqeB"
                     /locus_tag="VFF70_06470"
     CDS             complement(164..964)
                     /gene="yqeB"
                     /locus_tag="VFF70_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HZY44380.1"
                     /translation="MKTDQPHILLRGGGDLGTGAALRLHRASYRVLITELAQPLVIRR
                     AVAFASAIYDGSIGVEGVIAQRIDQLDQADRVWSIDHIPVLADPDRSISARWQPAVLV
                     DAIMAKINVGTHLHDAPIVIALGPGFTVGLDCHAIVETNRGHNLGRVYYQGSAEVDTG
                     RPGKIGGEDNKRTLRAPIEGLFRAEKKIGDKVVAGDVIARIGSFSIKTEIDGVVRGVL
                     HNDVAVAAGAKVADVDPRGEASYCFSVSDKAWAVGGGVLEAVMFLSRA"
     gene            complement(954..2297)
                     /locus_tag="VFF70_06475"
     CDS             complement(954..2297)
                     /locus_tag="VFF70_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44381.1"
                     /translation="MTESVIETAALESAVPMVDTALVDVPHLKRNFFLGVFNGAMFNF
                     AETLMSVDTVLTWFVQQLGGSYFLIGLVGPMRDAGWYLPQLFVSKWLEKQPHKLPQYR
                     SMAIIRCMAWLTWTLAALFIRDFSLLLFLFFVAYAVNAIASGLSGLSFMDIVAKTIPP
                     QRRGSYFGGRLFFGAILALGASVIVSLTLDQNNSVDFPRNIGWLFLLSWIAATAGLIA
                     FGYVIEPPGDVRLEETTMRSHVRRAARLPRHNQDFRYFLIARVLLMLSYVATPFYSVY
                     SIEVLHSPVEIIGIYIAVRTLTALLINPVWGRLSDRHHNRLVVRIATVIGMLVPIWAI
                     FAPLLAPLLHIGLTYIFVPIFALLGLYDTGVGIGENNLMFELSPDDDRAIHIGLTNTV
                     MGVAYFSTILSGLLVTWLGYNGVFILALIFFVVSLWAISRVGEPRELEAEMAIEN"
     gene            2447..>3980
                     /locus_tag="VFF70_06480"
     CDS             2447..>3980
                     /locus_tag="VFF70_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY44382.1"
                     /translation="MGGLDTRQIHGSKSTEGLEGALQQGRILQSRYEVLGILGLGGMS
                     AVYQGRDKRFPNVVKLCAIKEMKSHSLDPQMRAVAIQNFEREANILATLSHSAIPKVY
                     DYFSEEARSYLVMEFIEGRDLEAIINNTPGFLPQEQVLDWAIQLCDVLTFLHKHTPPV
                     IFRDMKPSNVMLDDHGQLRLIDFGIAKNFQPGQKGTMIGTEGYSPPEQYRGVADQRTD
                     IYALGATLHHLLTKQDPRVEPPFSFHERPINAANSGISPEFAQMIMRMLEYEPDRRFN
                     SAEEMKRSLAALRPATSTQFGAAALNFHSGDVTPLWTFACEDEIRSTPALNNEVVYVT
                     AYDHNLYALDAGTGKFLWKYPTDGGIAASPLLHDDRVLIGSDDRVVYCINAQSGRIVW
                     SCPTQGRIRSTPRVEFGHVFFGSDDRFLYAVNFLNGRVAWKVETEGPIRSSAAISDDT
                     IFIGSEDGGLYCLDIRGTIRWRHRSKRAITSSPALDAESKLVIVGSQDGTVYGLDMAS
                     GWV"
BASE COUNT         1016 a         1093 c         1042 g          829 t
ORIGIN      
        1 cgcgacaagg aaatgatttt gcgcgtatcg acgcgtattg caagatacag aaaattattg
       61 tagtaggggc gggtcattcc cacaggagaa tcattgaagt tcagaacgtc gaatttcagc
      121 atggcgttaa ttcacaaaaa attgtcgggg gaatgccgcg cccctacgca cgtgataaga
      181 acatcaccgc ttccaacact ccgccgccca ctgcccacgc tttgtccgac acgctgaaac
      241 aataactcgc ttcaccacgc ggatcgacat cggccacttt ggcaccagcc gcaacggcca
      301 cgtcattgtg cagcactccg cgcaccacac cgtcgatttc ggttttaatc gaaaacgatc
      361 cgatgcgagc aatcacatcg ccggcgacca ctttgtcccc gatttttttt tctgcacgaa
      421 acaagccttc gatcggcgca cgcagcgtgc gcttattgtc ctcaccgccg attttacccg
      481 gcctaccggt atcaacctcc gctgatccct gatagtaaac tctacctaag ttgtggcctc
      541 tattcgtttc gacaatcgcg tgacaatcga gccccacggt aaagcccggc cccaacgcaa
      601 tcacgatcgg cgcgtcatgc agatgggtgc cgacgttgat tttcgccatg atcgcatcga
      661 ccagtaccgc gggctgccat cgagccgaga tcgaacgatc gggatcagcg aggactggaa
      721 tgtgatcgat ggaccaaact cgatcggcct gatcgagctg atcgatccgc tgcgcaatca
      781 cgccttccac tccgatcgag ccgtcataga tcgccgaagc aaacgccaca gcgcggcgaa
      841 tcactaacgg ctgcgcgagt tccgtgatca agacgcgata gctcgcacgg tgaagtcgga
      901 gagcagcccc tgtgcctaaa tcgccgccgc cgcgcaagag aatatgtggc tgatcagttt
      961 tcaatcgcca tctccgcttc gagttcgcgc ggttcaccca cacgcgagat cgcccacagc
     1021 gaaacaacaa agaatatcag cgcgagaata aacacgccgt tataacccag ccatgtgacc
     1081 aataagccgc tgaggatggt gctgaagtaa gctacgccca tgacagtgtt ggtcaagccg
     1141 atatggatcg ctcgatcgtc gtcgggcgac aattcaaaca tcaagttgtt ttcaccgatg
     1201 ccaacgcctg tatcatataa acctaacagg gcaaagatcg gaacaaagat gtaagttagt
     1261 ccgatgtgca gcagcggagc aagaagcggc gcgaagatcg cccagatggg aacgagcatg
     1321 ccgatcacag tggcgatccg cacaaccaag cgattatgat gccgatcgct taagcggccc
     1381 caaacgggat tgatcaataa agctgtaagt gtcctgacgg caatataaat gccaatgatc
     1441 tccaccggcg aatgtaagac ttcgatcgag tagaccgagt aaaacggcgt ggcaacgtaa
     1501 gagagcatga gcagcacacg cgcgatcaag aagtatcgaa agtcctgatt gtggcgcggc
     1561 aaccgggcag cgcggcgcac gtgcgatcgc atggtggttt cttcgagacg cacatcgccg
     1621 ggcggctcaa tcacgtaacc aaaggcgatt aaacccgcag tggcggcgat ccatgataag
     1681 aggaacaacc agccgatatt gcgcggaaaa tcgaccgagt tgttttgatc caacgttaag
     1741 ctgacgatca cgctggcgcc taaagctaag atcgcgccga agaataatcg tccgccaaaa
     1801 tatgatccgc gccgctgcgg cgggatcgtc ttagcgacga tgtccatgaa cgacaagccc
     1861 gacaagccgg aggcgatggc attgacagca taagcgacga agaataagaa gagcagcagc
     1921 gaaaaatctc tgatgaacaa cgccgcgagc gtccacgtca gccaagccat acaacgaatg
     1981 atcgccatgc tgcgatactg cggcagtttg tgcggctgtt tttccagcca cttggaaacg
     2041 aacagctgcg gcaaatacca gccagcatca cgcatgggac cgaccaagcc aatcaagaaa
     2101 tatgaaccgc ccaattgctg gacgaaccag gtcagtactg tatcaactga catcaaggtt
     2161 tccgcaaagt tgaacatggc gccattgaac acgcccagga aaaaattccg cttgaggtgc
     2221 ggaacatcaa ctaaggccgt gtccaccatc ggcacagcag attcaagtgc agccgtctca
     2281 ataacagatt cagtcacgta cagctccagg acaattcgat tccgggttgg attataacac
     2341 cgcaagacga tcgttcgtat atgttttgca tctgctgttg atttacgtta taatccataa
     2401 actacactgc tgaaagcatc gatctcgatt aggatttgat taggtcatgg gcggcttaga
     2461 tactcgacaa attcacggtt caaaatcgac cgaggggtta gaaggcgcgc tgcaacaagg
     2521 ccgcatcttg caaagccggt atgaagtgtt gggtatcttg ggcttgggcg ggatgagcgc
     2581 ggtgtatcaa ggccgcgata aacgctttcc gaatgtggtt aagctgtgcg ccatcaaaga
     2641 aatgaagagt cattcgctcg atccgcagat gcgagcggtg gcgattcaga acttcgaacg
     2701 tgaagccaat attctggcca cgctcagtca ttccgctatt cccaaagtct acgattattt
     2761 ttcagaagaa gcccgctcgt atttagtgat ggagtttatc gaagggcgtg acctggaagc
     2821 gatcatcaac aacacgcctg gcttcctgcc gcaagaacaa gttctcgatt gggccattca
     2881 gctgtgtgat gtgctgacct ttctacataa gcacacaccc ccggttatct tccgcgatat
     2941 gaaaccgtcc aatgtgatgt tggacgatca cggccagctg cgtttgatcg attttggcat
     3001 cgctaaaaat ttccagccgg gccaaaaggg gacgatgatc ggcacagaag gttacagccc
     3061 gccggagcag tatcgcggcg tggccgatca gcgaactgac atatacgcgc ttggggcgac
     3121 gctgcatcat ctgctgacca aacaagatcc ccgcgtcgaa ccacccttct cgtttcacga
     3181 gcgtccgatc aacgcggcga attcgggcat ctcgcctgaa tttgcgcaga tgattatgcg
     3241 catgttggaa tatgaacccg atcgacgttt caactcggcg gaagagatga aacgtagtct
     3301 ggccgcgctc cgccccgcaa cttcaaccca gttcggtgca gccgcgctca atttccacag
     3361 cggggatgtg accccattgt ggacgtttgc ctgtgaagac gaaattagat cgacccccgc
     3421 cctgaacaat gaggtggtgt acgtaacggc ttacgatcac aatttgtacg cactggatgc
     3481 cggcactggc aaatttttgt ggaagtatcc caccgacggc ggcatcgctg cttcgccgct
     3541 cctccacgac gatcgcgtct tgatcggctc agacgatcgc gtggtatact gcataaatgc
     3601 tcaatctgga cgcattgtct ggtcgtgccc gactcaaggt cgcattcgat cgacgcccag
     3661 ggtggaattc ggccatgttt tcttcggctc agacgatcgg tttttgtatg cggtcaattt
     3721 tctaaacggc cgcgtcgctt ggaaagttga gaccgaagga cctattcgat catcagccgc
     3781 gatcagcgat gacactatct tcatcggcag cgaagacgga gggctgtact gcctggacat
     3841 tcgaggcacc atccgttggc gtcatcgatc gaagcgagcg attacctcat cgcccgcgct
     3901 ggatgctgag agcaagttag tcatcgtcgg ctcacaagat ggcacggttt atggcctcga
     3961 catggccagc ggctgggtga
//
LOCUS       DASRTJ010000317         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_56555,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000317 DASRTJ010000000
VERSION     DASRTJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56555"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFF70_06485"
     CDS             complement(<1..230)
                     /locus_tag="VFF70_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZY44383.1"
                     /translation="MNMPNFLIIGAHKAGTSAMHAYLKQHSQIYMSPMKEPNFFAWGE
                     DRFPDFQGPGDKTWFSRNTLRRLEDYKAEFSGA"
     gene            complement(256..1980)
                     /locus_tag="VFF70_06490"
     CDS             complement(256..1980)
                     /locus_tag="VFF70_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY44384.1"
                     /translation="MLLWVFLATALIGVWAAWDPNAAWAKFYLIVGAIGLYYAIAHQP
                     DLDHVFTILVFFGIFGVGLALYFFVTNDWSAQTTKIAFLASIGKGLAALLPSLAAHQL
                     HPNVAGGMLGLVLPVYVPLFILSRKRRSKRSHSTRWRWITWLWIVAAWIVLLAELVSE
                     SRGAWLGIAITGVMWLAWRFLGWLLHARPIERMWRLRVLIMGAVWLIGAAAAIAVISC
                     LLNRDSSGLGTLSSRVGLLRDGLLLARDYPLTGAGLGMFQLQHSTYILLIHVPYIIHT
                     HDLLVNILIEQGLFGLLAYLGLAAVSISTALRSLRYATSELALIIEASFAMLLVIWLH
                     GWVDDTLYGSRGLLLLWAPLAMIGVCYRLQSQALPESQPSTRRWLKPALGVALAALVI
                     LSIVWGRPVVASAFSNLGSVEQSRVELSHYDPDRFDKPSLDRIRQTQNLDRSIGLFEH
                     ALSIDPANLTAHQRLTGIDLSRGQYSAALDQIQLAWMAGYHDAVTRLLLGDAYVANGQ
                     VAQGVEAVKDLPEAIRRLEFQAFYRYTAAKDYRRAADAWAAVVALDPGNQAAIKAQAD
                     AARRVVQP"
     gene            complement(2002..2190)
                     /locus_tag="VFF70_06495"
     CDS             complement(2002..2190)
                     /locus_tag="VFF70_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44385.1"
                     /translation="MMNHSAWFKRNLPYIEFAIAIIAASLWYFQGGAVWYTGTFPGAW
                     PIVLLAAVWLIRWSINGF"
     gene            complement(2304..2377)
                     /locus_tag="VFF70_06500"
     tRNA            complement(2304..2377)
                     /locus_tag="VFF70_06500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2341..2343),aa:Val,seq:gac)
     gene            2511..>2699
                     /locus_tag="VFF70_06505"
     CDS             2511..>2699
                     /locus_tag="VFF70_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZY44386.1"
                     /translation="MSSTHWPVVGHEWAVLNLDRSVAEHRAAQAYLISGPAHIGKTTV
                     ARALALALNCESKTDRPDL"
BASE COUNT          733 a          753 c          648 g          565 t
ORIGIN      
        1 gcccctgaaa attcggcttt ataatcttca aggcgcctaa gcgtattccg ggagaaccaa
       61 gttttatctc cgggaccttg aaaatcagga aaccgatcct cgccccaggc aaagaaattg
      121 ggctctttca tcgggctcat atagatttga gagtgctgtt taagataagc gtgcatagct
      181 gatgtacccg ctttgtgagc accaataatt aaaaaattag gcatattcat agtaggaagt
      241 ttctcttgca aaagatcaag gttgaacaac ccgccgcgcg gcatcggcct gagctttaat
      301 cgccgcttga ttgcccggat ccagtgccac gactgccgcc cacgcatccg ccgcgcggcg
      361 ataatcttta gcagcagtat agcgataaaa tgcttgaaat tccaagcgcc taatcgcttc
      421 tggcaaatct tttacggctt caactccttg agccacttgc ccgtttgcca catacgcatc
      481 acctaatagc aaacgggtca cggcatcgtg gtagcccgcc atccacgcaa gttgaatctg
      541 atcgagcgcc gctgaatact gaccgcgcga tagatcgatc ccggtcaagc gctggtgcgc
      601 ggtaaggttc gctggatcga tcgagagggc gtgctcgaac aaaccgatcg atcgatctaa
      661 gttttgcgtt tgccgaatcc gatcaagtga tggcttgtcg aaacgatcgg gatcataatg
      721 actcaactca acacgcgact gctcaacact tcccagattt gagaaagccg atgccacaac
      781 gggccgcccc catactatac tcagaattac taatgcggca agcgccacac ctagcgcagg
      841 tttcaaccag cgccgcgtgc tcggttgtga ttcaggcagt gcttgagatt gcaggcgata
      901 gcaaacacca atcatcgcca gcggcgccca taacaacaac aagcctcggc tgccatacaa
      961 ggtgtcatcg acccagccgt ggagccagat cacgagcagc atcgcgaagg acgcttctat
     1021 gattaatgcc aactctgatg tggcatagcg gagtgaacgc agcgcggtgc taatcgagac
     1081 tgcagctaac ccgaggtaag ccagtaaacc gaacagtccc tgttcgatca gaatgtttac
     1141 cagtaagtcg tgcgtatgga tgatatacgg cacatgaatc agcaagatgt aagtcgaatg
     1201 ctgcaattga aacattccca gaccggcacc ggtcaacgga tagtcgcggg ccaataacaa
     1261 gccatctcgc aagaggccga cgcggctgct caatgttccc aagccggacg aatctcgatt
     1321 caacaagcag ctgatgactg caatcgcggc tgccgcgccg atcagccaca cagcgcccat
     1381 gatcaagaca cgcagccgcc acatacgttc gatcgggcgt gcatgcaata accagcccaa
     1441 gaatcgccaa gccaaccaca tgacacccgt gatcgcgatt cctaaccatg cgccgcgcga
     1501 ttccgaaact aattcggcta ataaaacgat ccaggccgcg acgatccaca accacgttat
     1561 ccatcgccac cgtgttgagt gagatcgctt cgatcgacgt ttgcggctga gtataaacag
     1621 cggcacatac acgggcaaca ctagccccaa catgccgccg gccacattcg gatgaagttg
     1681 atgcgcggca agtgaaggca gcaacgctgc cagaccttta cctattgagg caaggaaagc
     1741 aatcttagtg gtttgagccg accagtcatt cgttacaaaa aaatacagcg ctaatccaac
     1801 gccgaagatt ccaaagaata ccaagatcgt aaagacgtga tcgagatcgg gttgatgcgc
     1861 gatcgcgtaa tacaaaccga tcgccccaac gatcaagtaa aatttagccc aagccgcatt
     1921 gggatcccaa gccgcccata ctccaatcag cgccgtcgcc aagaacaccc acagcagcac
     1981 atcgatcaat tatgggtgaa gctagaaccc gttgatcgac cagcggatta gccagacggc
     2041 cgccaacaaa acgatcggcc acgcgccggg gaatgtaccg gtgtaccaca ccgctccgcc
     2101 ttgaaaatac cacaagcttg cggcgatgat cgcgatggcg aattcaatat agggtaaatt
     2161 acgtttgaac cacgcgctat gattcatcac gctttttctt cactaaactt ttgagggtta
     2221 aacaaaaaca gccctcactt ttcagcaagt ggcgggctat tctgatataa cgtggctgaa
     2281 gatcattttt aacaagtgcc gagtgggcgg tatagggctc gaacctacga cctctcggat
     2341 gtcaaccgag tgctctaacc agctgagcta accgcccgaa gtttttgtat tatacccagc
     2401 tcagccctat cggtcaaccg aattaaattt gatcgaggat taggaacaaa taatcagagt
     2461 tagtcatttg gcatcgatca gttgtccttg ttcattggta taatatcgtc atgtcctcaa
     2521 ctcactggcc cgtggttgga cacgagtggg ccgttctcaa tctcgatcga tcggtggccg
     2581 aacaccgcgc ggctcaagct tatttaatca gcggcccagc ccacattggc aagacgacgg
     2641 tggcccgcgc actggcactt gctttaaact gtgaatcaaa aacggatcgg cccgatcta
//
LOCUS       DASRTJ010000318         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_18488,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000318 DASRTJ010000000
VERSION     DASRTJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18488"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFF70_06510"
     CDS             <1..891
                     /locus_tag="VFF70_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44387.1"
                     /translation="TLARSPRFQASALPHRETLTIDGGRMRVTVSPDIARSTDVRIIS
                     PQATTVLHEGSGSIDVTNDELQVTIRDGTAGVTALGATVTLTQSQRTQVVLGQPPSGV
                     LSPERNLIINGNFRDSLDGTWEISNDLQAPNEPPGSVSILAESGRRLAVFDRTGVYHA
                     ETDLKQTINKDVRDFRSLKLHFVVQVSNQDVPVCGQAGSECPLMMRIDYKDVNGTDRS
                     FIQGFYNVLDPGNVNPNYCTSCGSRLEHIHVSKDFPYTYELGDLMTQLQPTQITAITF
                     YASGHSYRSAVAEIELLGEQ"
     gene            complement(929..1636)
                     /gene="radC"
                     /locus_tag="VFF70_06515"
     CDS             complement(929..1636)
                     /gene="radC"
                     /locus_tag="VFF70_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZY44388.1"
                     /translation="MDDEPIDYHPTIKDLPSGERPRERLAREGAGSLSNAELLAILFR
                     VGVDGENVLELATRLLARYQGLAGLARASFVELQNEHGLGEAKVSALKAALELGRRLT
                     IASPDDRLQIHSPQDAANLLMSEMSMLEQEHLRVMLLDTRNRVLSTPTIYKGNLNTSV
                     VRVSEIFRDAIRQNAAAIIVAHNHPSGDPTPSPEDVAVTRNIISAGKLLDIEVLDHLV
                     IGQQRYVSLKERGLAFE"
     gene            1813..>2158
                     /locus_tag="VFF70_06520"
     CDS             1813..>2158
                     /locus_tag="VFF70_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY44389.1"
                     /translation="MDIGSILLAIVAFIGAVLAAFWLTLIIWAFRDMRSRSRDIFAQI
                     LAAMVVAILNLPGVGIYLLMRPKETLAEAYERSLEEEALLQGIEESLNCPGCGNRVHG
                     DWLVCPNCHTKLK"
BASE COUNT          482 a          576 c          586 g          514 t
ORIGIN      
        1 accttagccc gatcgccgcg ctttcaagcc agcgctctgc ctcaccgtga aacgctcacg
       61 atcgatggcg gccgtatgcg tgtgacggtt tcaccggata tcgctcgatc gacggatgtc
      121 agaatcatca gcccgcaggc cacgactgtc ctgcacgaag gctcaggttc gatcgacgta
      181 acgaacgacg aattacaagt gactatccgt gatgggacgg ctggcgtcac ggcgctaggt
      241 gcaaccgtga cattgactca atcgcaacgc acacaagtgg tgctgggtca gcccccgtcg
      301 ggtgtgctct cgcccgaacg aaacttgatt atcaacggca atttccgtga ttccctggac
      361 ggaacctggg aaattagcaa tgatctgcaa gccccgaatg aaccgcccgg ttcggtgagt
      421 attttagcgg agagcggacg ccgactggcg gtgttcgatc gaaccggtgt gtatcatgct
      481 gaaactgatc ttaagcaaac gatcaacaaa gatgtgcgag acttccgatc gttgaagttg
      541 cactttgtcg ttcaggtttc caatcaagat gtgccagtgt gtggtcaggc cgggagcgaa
      601 tgtccgctga tgatgcgcat cgattacaaa gatgtcaatg gcactgatcg aagtttcatc
      661 caaggttttt acaacgtgct cgatcccggc aacgttaatc cgaattactg tacttcgtgc
      721 ggcagccggc tcgagcacat ccacgtgtcg aaagattttc cgtacacgta cgaactcggc
      781 gatttaatga cacaacttca accgacccag atcaccgcca ttacctttta cgcttcgggc
      841 cacagctatc gatcggcggt ggccgagatc gagttgttag gcgagcagta accccatctg
      901 catctccccc gcttcgcagc ggaggaagtc actcaaatgc caggcctctt tctttaagac
      961 tgacatagcg ttgttgcccg atcaccagat gatcgagcac ttcaatatcc aacagcttac
     1021 cggccgagat aatattgcgc gttacggcta catcttccgg cgacggcgta ggatcaccgg
     1081 acgggtgatt gtgcgccacg atgatcgctg cggcgttctg ccggatggcg tctcgaaata
     1141 tttcagagac gcgcactacc gatgtgttta agttgccctt atagattgtc ggcgtgctca
     1201 agacgcgatt gcgcgtatcg agcaacatga cgcgcaagtg ctcttgctca agcatactca
     1261 tttcactcat cagtagattg gcagcatctt gcgggctgtg gatttgaagt cgatcatccg
     1321 gggaggcgat cgtcaagcgg cggcccaatt caagcgctgc tttcaacgca gatactttcg
     1381 cttcacctaa gccgtgctca ttctgcaatt cgacaaatga cgcacgcgcc aagcccgcta
     1441 aaccctgata gcgcgcgagc aatcgagtgg ctaattcaag gacattttca ccgtccacac
     1501 caacacgaaa caggatggcg agtaactcgg cgtttgataa gctgccggcc ccttcacggg
     1561 ccagccgctc gcgtggacgt tcgccgctgg gcaggtcttt gatggtggga tgataatcga
     1621 tcggttcgtc gtccatgccg cctccttgaa gccaaccaga ttttatttgc gcggaagatc
     1681 aatcggtggg cagactatac ttcaaatcta gcgagcatgt caaacgcttg gcgtccccac
     1741 tcatccgtca tttgtcattg atcgctggtc attgtataat ggcggccact cagcaaggag
     1801 atcgatcatt caatggatat tggaagcatt ctgttagcca ttgtcgcctt catcggcgcg
     1861 gtgctggcag ctttttggct gacgttgatc atttgggctt tccgcgatat gcggtcgcga
     1921 tcgcgcgata tctttgccca gattttagcg gcgatggtgg tcgcgatcct caatttgccg
     1981 ggcgtgggaa tttatttgtt gatgcgcccg aaggaaacac ttgctgaggc ctacgagcga
     2041 tcgctggaag aggaagcgtt gctgcaaggc attgaggagt cgttgaattg tccgggatgc
     2101 ggcaatcgtg tgcatggcga ttggctggtg tgccccaatt gtcacaccaa gttaaaga
//
LOCUS       DASRTJ010000319         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_56572,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000319 DASRTJ010000000
VERSION     DASRTJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56572"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1403)
                     /locus_tag="VFF70_06525"
     CDS             complement(72..1403)
                     /locus_tag="VFF70_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZY44390.1"
                     /translation="MLEEYLDRYIDNIASALKKLDRRAVWAVIEELMTAWREHRQVFL
                     LGNGGSAALASHMANDLCKTSIVNDQPRLRAIALTDNVPLLTAWANDNGFEHIFSEQL
                     YNLCQPGDVVIAISCSGNSANVLQALRAARELKARTIGFTGDIGGQLKDLVDVCVFAP
                     AEMIWPQEDVHLILDHVIASILQLWIAAIAEKSAQPMRAMILAAGEGTRLRPLTLDKP
                     KPMLPVNDKPLLHYTIKWLREAGIHDVAVNLHYRPASIVDHFGDGAHWGVNLSYSHED
                     SLLGTAGALRKLNGFAISGSLVVVYGDVLTNLDLRGLMAFHRQQLARDPLLGVTLSLQ
                     RLPNPIGKGLVELDERGRVTRFVEKPAAHEMFGDLVNAGVMIIEPGVIDRIPRDTFYD
                     FGQHLLPDLLHSDRSIYGWIIPANAYLIDIGTPENYAQAQRDWPMAASDHA"
     gene            complement(1519..1592)
                     /locus_tag="VFF70_06530"
     tRNA            complement(1519..1592)
                     /locus_tag="VFF70_06530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Val,seq:gac)
     gene            1778..>1966
                     /locus_tag="VFF70_06535"
     CDS             1778..>1966
                     /locus_tag="VFF70_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZY44391.1"
                     /translation="MSSTHWPVVGHEWAVLNLDRSVAEHRAAQAYLISGPAHIGKTTV
                     ARALALALNCESKTDRPDL"
BASE COUNT          454 a          571 c          504 g          437 t
ORIGIN      
        1 cgttttaacg tagtccgttc gattccggtt gatgacgccg tctcgatcga gaaaaatagc
       61 gggccgcatg atcacgcgtg atcgctcgcc gccatcggcc aatcgcgctg agcctgcgcg
      121 taattctccg gcgtgccgat atcgatcaag taggcattcg caggaatgat ccagccatag
      181 atcgatcgat ccgagtgcag caagtccggc agcaagtgct gtccgaaatc ataaaaggtg
      241 tcgcgcggaa tccgatcgat cacgcccggc tcgatgatca tcacgcccgc gttgaccagg
      301 tcgccgaaca tttcatgcgc cgccggtttt tccacaaagc gcgtgacgcg gccccgctca
      361 tccagctcga ctagaccttt cccgatcggg ttgggcagcc gctgcaagct taacgtcaca
      421 cccagcaacg gatcgcgcgc cagctgctga cgatggaacg ccatcaagcc gcgcagatcc
      481 aaattggtca gcacatcccc gtacaccacc accaatgaac cgctaatcgc aaagccattt
      541 aactttcgta aggcgccggc cgtgcccagc agtgagtcct catgcgaata agatagattc
      601 acaccccagt gcgctccatc gccgaagtga tcgacaattg atgcaggccg ataatgcaga
      661 ttgactgcca cgtcatgaat gcccgcctcg cgtagccact tgatcgtgta gtgcaagagc
      721 ggcttatcgt tgacaggtag catgggttta ggtttgtcca gcgtcagtgg gcgcaggcgc
      781 gtgccttcac ccgccgccaa gatcatggcc cgcatcggtt gggctgactt ttcagctatc
      841 gccgcaatcc acagctgcaa gatgctggcg atgacatgat ccaaaatcag atggacatct
      901 tcttgcggcc agatcatttc ggcgggcgca aagacacaca cgtccaccag atctttcagt
      961 tgaccgccga tatcgccggt aaagccaatc gtgcgcgctt ttaactctcg cgccgcgcgc
     1021 agcgcttgca agacattcgc actgttgccg ctgcacgaaa tggcgattac cacatcaccg
     1081 ggctggcaca agttgtacag ctgctcggaa aagatatgct cgaagccgtt atcgttggcc
     1141 cacgccgtca gcaatggaac gttatccgtt agggcgatgg cacgcaagcg cggttgatca
     1201 tttacgatcg aagtcttgca cagatcgtta gccatatgtg aagccagggc ggcgctaccg
     1261 ccgttgccta acagaaacac ctgccgatgc tcgcgccagg cagtcatcag ttcctcgatg
     1321 acggcccaga cggcgcggcg atccaacttc ttcagcgccg aggcaatgtt gtctatatag
     1381 cgatcgaggt attcctctaa catgccgtga tccttgcgtt aaacaagttt aaacaaaaac
     1441 agccctcact cttcagcaag tggcgggcca ttccggacaa aacttgaatc actgtcatcg
     1501 ctgagaatat gtcctcagtg ggcggtatag ggctcgaacc tacgacctct cggatgtcaa
     1561 ccgagtgctc taaccagctg agctaaccgc ccgatgtttt tgtattatac ccagctcatc
     1621 ctgatcggtc aaacgaatca ctctcgatcc gtgatcaggc tgcctgtggc aagatgcacg
     1681 ttgtaagtta tttagtgttg gtgatttgtt attcgtcatt gcacactttg ttcattaaga
     1741 attgtgcatt gtgcaatgat aattgtataa tgacagcatg tcctcaactc actggcccgt
     1801 ggttggacac gagtgggccg ttcttaatct cgatcgatcg gtggccgaac accgcgcggc
     1861 tcaagcttat ttaatcagcg gcccagccca cattggcaag acgacggtgg cccgcgcact
     1921 ggcacttgct ttaaactgtg aatcaaaaac ggatcggccc gatcta
//
LOCUS       DASRTJ010000320         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_245652,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000320 DASRTJ010000000
VERSION     DASRTJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245652"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFF70_06540"
     CDS             complement(<1..863)
                     /locus_tag="VFF70_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HZY44392.1"
                     /translation="MNIDSSPWMKQLEQRSPVQRFFSHGLFAVGLIIALVGVLALGYN
                     ATVGSITLKIDGQTRVIRTNQRSIENILRDADITIQPEDLVVPDYSATLDDHQSTITV
                     QHARLITIDVDGQTKQFRSRATALDELFKQANIEVGAHDRISVNGRVANRGSDTTQLN
                     APGVIDLKRVTIEIKRAVPIEIKIGTNAQKFESAADTIGQALAEANVQIYLADRITPD
                     LATPLQSNLQVVIEPSMPINVQVDGQLLKTRTRRSAVGEVLNDLGVTLLGEDYTQPTV
                     DASVESGAVVNV"
     gene            complement(946..1045)
                     /gene="ffs"
                     /locus_tag="VFF70_06545"
     ncRNA           complement(946..1045)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFF70_06545"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1160..1249)
                     /locus_tag="VFF70_06550"
     tRNA            complement(1160..1249)
                     /locus_tag="VFF70_06550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1211..1213),aa:Ser,seq:gga)
     gene            complement(1472..2839)
                     /locus_tag="VFF70_06555"
     CDS             complement(1472..2839)
                     /locus_tag="VFF70_06555"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HZY44393.1"
                     /translation="MAKATLHFPPDFKWGTATAAHQVEGGNTNNDWWAWEQGEGHIKG
                     GQKSGQASDWWGDGFETDMDFAAQMNNTGHRMSIEWSRIEPKEGQWDDAAIDHYRSML
                     KSMRARNIEPMITLHHFTNPIWLAERGGWETIEVMPLFERFVTKVVESLKDLCDLWIT
                     INEPNWYVPGGYMQDIDKIDGNLPQGTFPPGKNDLGLALKVADNMLLGHAAAYHAIHR
                     IQPAARVGIAPNFRLFEPYNPKSRLDRFVARNRDRMFNQVIIDAVTRGKIGRPIGFAR
                     RMGSLKNTIDYLGVNYYTREFTRFNRSSADTILFGQGIIKPDADMSDGNFGELHAHGL
                     FRFLKRLDRIGKPLYITENGLPDADDDQRPAFLIEHLRSVWLAINQNVPVMGYYHWSL
                     VDNFEWAEGWNLRFGLIELDPQTQQRTLRQSGKLYGEICKSNKIDTELVKRYAPEIVS
                     KIFPG"
     gene            complement(2841..3401)
                     /locus_tag="VFF70_06560"
     CDS             complement(2841..3401)
                     /locus_tag="VFF70_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44394.1"
                     /translation="MLIRPLVSHDLPHLQNIDANFTSDRFLDVRKSVDGFSITWQLIE
                     RPLNPPFVSTDYNIPDTEIEELADRLRTNDGLYLAVEDEDKLIAFLDMPREHWRNAAL
                     ISNLYVDQKFRRRGVGAELINRAVHYAQENSIRAIWLETQSNNYPACQFYRSRGFEVC
                     GLDDHFYSNEDMALKEVALFWWKEIN"
BASE COUNT          778 a          993 c          944 g          864 t
ORIGIN      
        1 acattgacaa ctgcgcctga ttcaactgac gcatcaactg tgggctgcgt gtaatcttca
       61 cccaacaacg tcacgcccag atcattcaac acttcgccca cggccgaccg gcgtgtgcgc
      121 gttttcaaca actgcccatc gacttggaca ttgatcggca tcgaaggctc gatcacaacc
      181 tgtagattcg attgaagcgg tgtggcgaga tcgggcgtaa tccgatcagc cagataaatc
      241 tgcacgttcg cttcggccaa tgcttgcccg atcgtatcgg cggcgctttc aaatttttgt
      301 gcgttagtgc cgatcttgat ctcgattggc accgcgcgct tgatctcgat cgtcacacgc
      361 ttgagatcga tcacgccggg cgcattgagt tgggtggtat cgctgccccg attggcgacg
      421 cggccattca cactgattcg atcgtgcgcg ccgacctcaa tatttgcctg cttgaagagt
      481 tcatccaagg ccgtcgcgcg cgatcgaaat tgtttcgtct ggccgtccac gtcgatcgtg
      541 atcaatcgag cgtgctgcac cgtgatcgtg gactgatgat cgtcgagcgt ggcagaataa
      601 tcgggcacga cgaggtcttc cggctggatc gttatatccg catcgcgcaa aatattttcg
      661 atcgaacgtt gattggtgcg gatcacgcgc gtttgtccat cgatctttag cgtgatcgat
      721 ccgacggtgg cgttatagcc gagcgccagc actccgacca gcgcgatgat caagccaacg
      781 gcaaagagcc cgtgcgaaaa aaatctttgc acggggctgc gctgttcgag ttgtttcatc
      841 cacggcgacg aatcgatgtt catttccatc atctcctccc ctgcgacatc tttttgtcgc
      901 gggggaggtt agggaggggg tgagcaactt atcctgcctt caatccagcc tcaggcctcc
      961 cggcgacacc cagcgaattc tccttatcgc tgcttccttc cggatctgac gaggttcaga
     1021 ggccgctgcc gcgccggacc caaagccgat cgaaggcagg gcaaggtgct caacacattg
     1081 aatgcggagt gcagaatgat gaatgcggaa taacacgcca ctcactccgc attccgaaat
     1141 ccgcactccg caatggtcat ggcggagagg gcaggatttg aacccgcggt gccttgcggc
     1201 acacgcgctt tccaggcgcg cgcgttaggc cagactacgc tacctctcca ttgggcttaa
     1261 tacggcggga attataacac aaggctcgat ggatattctc catcgagcgt tattgtttta
     1321 ggatcgtgtc atgctgagcg aagcatcact accgctgatt gacgtgaatg ccgatcgtca
     1381 gagagattct tcggcgcaaa caggtgcact tcaggatgac atgcagtcgt ctttcaactt
     1441 tacactttca acttgcttct tggcttttgc ttcatcccgg gaaaatcttc gaaacaatct
     1501 ccggcgcata tcgtttgacc aactccgtat cgatcttgtt gctcttgcaa atttcgccgt
     1561 acaacttgcc gctctggcgc agcgtgcgct gctgcgtctg cggatcgagt tcgatcaagc
     1621 cgaagcgcag attccagccc tccgcccatt cgaaattatc gacgagcgac cagtgatagt
     1681 agcccatgac cggcacgttc tgattgatcg ccagccagac cgatcgcaga tgttcgatca
     1741 agaaggccgg ccgctgatcg tcatccgcat ccggcaggcc attctcggtg atgtacagcg
     1801 gcttgccgat gcgatccaat cgcttgagaa agcggaacaa gccgtgcgca tgtagttcgc
     1861 cgaagttgcc atcgctcata tcggcgtccg gcttgatgat cccttggccg aataaaatcg
     1921 tatcggcgct cgatcgattg aagcgcgtga actcgcgcgt gtaatagttc acgccgaggt
     1981 agtcgatggt atttttgagt gatcccattc gccgcgcaaa accgatcggc cggccgatct
     2041 tgccgcgcgt gaccgcatcg atgatgactt gattaaacat ccgatcgcga ttgcgggcga
     2101 cgaatcgatc gaggcgcgac ttgggattgt acggctcgaa caagcggaag ttgggcgcga
     2161 tgccgacgcg cgcagcgggt tgaatacgat gaatcgcatg atacgccgcc gcatgaccga
     2221 gcagcatgtt gtccgcaact ttcaaggcga ggccgagatc attcttgccc ggcggaaaag
     2281 ttccctgcgg caaattcccg tcgattttgt cgatgtcctg catgtagccg cccggcacat
     2341 accagttcgg ctcattgatc gtaatccaca aatcgcacaa gtctttgagc gattcaacaa
     2401 ctttagtgac gaaacgttcg aacagcggca tgacctcgat tgtttcccag ccgccgcgct
     2461 ccgccagcca gatcggattg gtgaagtgat gcagcgtgat catcggctcg atgttgcgcg
     2521 cgcgcatcga cttgagcatc gatcgataat gatcgatcgc cgcgtcgtcc cattggcctt
     2581 ccttcggttc gatgcggctc cactcgatcg acattcgatg cccggtgttg ttcatctgcg
     2641 cggcgaaatc catatccgtt tcaaaaccgt cgccccacca atcggacgcc tggccggatt
     2701 tttgtccgcc cttgatgtgg ccttcgcctt gttcccacgc ccaccaatcg ttgttcgtgt
     2761 tgccgccttc cacttgatgc gcggcggtcg ccgtgcccca tttgaaatca ggcgggaaat
     2821 gtagagttgc ttttgccatg tcagttgatt tccttccacc aaaacaacgc cacttctttc
     2881 agcgccatat cttcattcga gtaaaaatga tcatcgaggc cgcatacttc aaagccgcgc
     2941 gatcgataaa actggcaggc cggataattg ttcgattgtg tttccagcca aatggcgcgt
     3001 attgagtttt cttgagcgta gtgcacagcc cgattgatta actcggcccc cacgccacgt
     3061 cggcggaact tctgatcgac gtacagattg gagatcagcg ccgcattgcg ccaatgctcg
     3121 cgcggcatat ctaagaatgc gattaactta tcctcatctt caacggccaa atacaatcca
     3181 tcatttgttc gcagccgatc ggccagttct tcaatttcag tatcgggaat attgtaatcg
     3241 gtgctgacga aaggtggatt gagcggtcgc tcgatcaact gccacgtgat gctgaaacca
     3301 tcgacgctct tgcggacgtc gagaaatcga tcggaggtga aattcgcatc gatgttttgg
     3361 agatgaggta agtcgtgtga aacgagtggc ctgatgagca tagcataatc agtgaatagt
     3421 cattagtgga aatgcactac taatgcaagt tacgcaggtt aagcgtcatt gcgaggaggt
     3481 cgttaggccg acgtggcaat ctcccgctcg atattgagat tgcttcgctt cgctcgcaat
     3541 gacggtgagt aacaacaatt actaattact atttatagc
//
LOCUS       DASRTJ010000321         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321399,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000321 DASRTJ010000000
VERSION     DASRTJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321399"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFF70_06565"
     CDS             <1..807
                     /locus_tag="VFF70_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZY44395.1"
                     /translation="AYVHMAMKSIFDPDIPNNDGSAAPIRLTAPEGTVVNCKFPAAVA
                     ARMQIGHFMTEIIYRALAQALPNRVIAASGGTPATQTIFYGRRNHQQPFHTVMIRGGG
                     LGAGGARDGEGSFIFPANGANTPVEIFESDTPLMIDRRELLIDSGGAGQQRGALGRRE
                     VFRVPADEYAPQPPVNLGIQSGRFRLPPDGLFGGRSGACAQFLVNDQAGNPYGLTRLQ
                     PGDVVIMDAAGGGGYGDPAQRDRDQLLRDVRDGKVSRESALRDYSVEIKD"
     gene            879..1310
                     /locus_tag="VFF70_06570"
     CDS             879..1310
                     /locus_tag="VFF70_06570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZY44396.1"
                     /translation="MKTIAQPYKFHTPVRVRFNETDLQGHVNFGHYLFYFDVGVTDYL
                     KAIGYSYQRMKADSVDMLYLESRAQYLSSAYFNEMLNIHTRIGHIGNSSMRFEFVVLA
                     APDGRAVADGHIAVVMIEPGTRQKIRVPDRLREAVARYENG"
     gene            1323..2345
                     /locus_tag="VFF70_06575"
     CDS             1323..2345
                     /locus_tag="VFF70_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZY44397.1"
                     /translation="MKAAIFHGAHQLLTLEDIPTPTPQAGELLIKVAGCGVCHTDLHY
                     LDHDVPTFKKPPLILGHEVSGVVAAIGASVNYFKDGDRVLAPAVYGCGHCKMCRTGRE
                     NICENMVMFGNHVDGGYAEYMLAPAKDVIPLPVEIPLIEGAIIADATTTPYHAVVNRG
                     KVQPGDAVVVFGCGGIGLNVVQIAAAVGGQVIAIDVVAAKLDWAKKLGAAATLNATGV
                     ERVDKEIRKLTNGGADIAFEAIGNPVTQAQAFASVRTGGRFVVVGFSDKPMTLDTGRV
                     MFREMEITGSLGCRAVDYPRVIELVRQGKIKVAELVTAKFPLDRINAAFDTLRRGEGI
                     RSVIVP"
     gene            2504..>2706
                     /locus_tag="VFF70_06580"
     CDS             2504..>2706
                     /locus_tag="VFF70_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44398.1"
                     /translation="MLIDFHIHVSRPEHELPWVMEFIESQYDGDLWALAEKVLTPTGL
                     RPFLQSCGIDRAGHSLAHIDRRHT"
BASE COUNT          574 a          728 c          812 g          592 t
ORIGIN      
        1 gcctacgtcc acatggcgat gaagagtatc ttcgatcccg acattcccaa caacgacggc
       61 agcgcggcgc cgattcggct caccgcgccc gaaggcacgg tggtcaattg caaatttccg
      121 gcggcggtcg cagcgcgcat gcagatcggc cacttcatga cggagattat ttatcgcgcg
      181 ttggcgcagg ctctgccgaa tcgcgtgatc gcggccagcg gcggcacacc tgcgacacaa
      241 acgatctttt acggacgacg taaccatcag cagccgtttc ataccgtgat gattcgcggc
      301 ggcggcttgg gcgcgggcgg cgcgcgtgac ggcgaaggca gttttatttt tccggccaac
      361 ggcgcgaaca cgccagtgga aatttttgaa agcgacacgc ccttgatgat cgatcggcgc
      421 gaactgctga tcgattctgg cggtgcaggc caacagcgcg gtgcgctcgg tcgacgcgaa
      481 gttttccgtg tgcccgctga cgagtacgcg cctcaaccgc cggtgaatct gggcattcag
      541 tccgggcgct tccgcctgcc gcccgatggt ttgttcggcg gccgatcggg cgcgtgcgca
      601 caatttctcg tcaacgatca agccggcaac ccgtacggcc tgacgcgtct ccagccgggc
      661 gatgtggtga tcatggacgc ggcgggcggt ggcggctacg gtgatccagc gcagcgcgat
      721 cgcgatcaac tgctgcgcga tgtgcgcgat ggcaaagtgt cgcgcgagag tgcgctgcgt
      781 gattatagtg tggagatcaa agactgatcc acgaatctcc aagaacaata aattcattcg
      841 tggagattcg tggataaaaa cttgagctgt cgttaatcat gaaaacaatc gctcagccct
      901 acaaatttca tacgccggtg cgcgtgcgct tcaatgaaac cgatctgcag ggccacgtca
      961 atttcggaca ttacttgttt tacttcgatg tgggtgtgac ggattatctc aaggcgattg
     1021 gctattcata tcagcgcatg aaagctgaca gcgtggatat gttgtatctc gaatcgcgcg
     1081 cgcagtatct gtcctcagcg tactttaatg aaatgctcaa cattcacacg cgcatcggcc
     1141 acattggcaa ttccagcatg cgctttgaat ttgtggtgct ggctgcgccc gatggtcgcg
     1201 ccgtagctga cggtcatatt gccgtagtca tgatcgaacc gggcacgcgc cagaaaattc
     1261 gcgtgcccga ccggttgcgc gaagccgtag cacggtatga gaacgggtag acgggagata
     1321 aaatgaaagc cgcaattttt catggcgcgc atcagctgct cacgcttgaa gacattccga
     1381 caccgactcc gcaggccggt gagctgttga tcaaagtcgc aggctgtggc gtgtgtcaca
     1441 ccgatctgca ttacctcgat catgatgtgc cgacgttcaa gaagccgccg ctgatcttag
     1501 gccacgaagt gtcgggggtt gtggcggcaa tcggagcaag cgtcaactat ttcaaagatg
     1561 gcgatcgcgt tctcgcgcca gcagtctacg gctgcggtca ctgcaagatg tgccgcacgg
     1621 gccgcgaaaa tatttgcgag aacatggtca tgttcggcaa tcacgtcgat ggcggttacg
     1681 ccgaatacat gctcgcaccg gcgaaagatg ttatcccgct gcccgtagaa attccattga
     1741 tcgaaggcgc gatcatcgct gatgcgacga ccaccccgta tcacgccgtc gtcaatcgcg
     1801 gcaaagtgca gccaggcgat gcggtcgtcg tcttcggctg cggcggcatt ggtttgaacg
     1861 tcgtgcagat cgcggcggcg gtgggcggac aagtgatcgc catcgatgtg gtcgctgcca
     1921 aattagattg ggcgaagaaa ctcggcgcag ccgcgacact caatgcgaca ggtgttgagc
     1981 gcgtcgataa agaaattcgc aaactcacga atggcggcgc ggatatcgct tttgaagcga
     2041 tcggcaatcc ggtgacgcag gcgcaggcct ttgcctcggt gcgaaccggc ggccgttttg
     2101 tggtcgtcgg tttttccgat aagccgatga cacttgatac cggccgcgtg atgttccgcg
     2161 aaatggagat caccggatcg ttgggttgcc gcgctgtcga ttatccgcgc gtgatcgaat
     2221 tggtgcggca gggcaaaatt aaagtggctg aactggtcac ggctaagttc ccgctcgatc
     2281 ggatcaacgc tgccttcgat acgctgcggc gcggtgaagg cattcgatcg gtgattgtgc
     2341 cgtaagtaaa gaacgtcatt gcgaggcgca tcttttcgcg ccgaagcaat ctccacgact
     2401 atcagctgag attgcttcgt cggcctacgg cctcctcgca atgacggctg agtggtgatg
     2461 ctattcgtag atttgtgtac gaaataaatt gtggagcgat tctttgttaa ttgattttca
     2521 cattcacgtc tctcgacccg aacatgaatt accgtgggtg atggaattca tcgagagtca
     2581 atatgacggc gacctgtggg cgctggccga aaaagtcttg acgccgactg gactgcggcc
     2641 attcctacaa tcatgcggca tcgatcgggc tgggcattcg cttgcgcaca tagatcggcg
     2701 acatac
//
LOCUS       DASRTJ010000322         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_18626,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000322 DASRTJ010000000
VERSION     DASRTJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18626"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFF70_06585"
     CDS             complement(<1..356)
                     /locus_tag="VFF70_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44399.1"
                     /translation="MSKLSVHITSGKRDGFSDWLKKCAEAGSPIGIVYSVNENIADDI
                     AKYSPTTTWVYRYQTDEFNRLPDGFFESDAVKNCETWMLKTRDSHHRTLMDNWRLNKA
                     DWFDPLNEPVPDIPVKA"
     gene            complement(484..1419)
                     /locus_tag="VFF70_06590"
     CDS             complement(484..1419)
                     /locus_tag="VFF70_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZY44400.1"
                     /translation="MANLKLDTRFNTKGSLIATWQGKASSRPRAMTAHVDTLGGMVKE
                     ILPNGRVRMNRIGGLAWTAAEGEGVSISTESGKVYRGTLQAQKASVHVHTRDVVEGQP
                     RTDDTMEIRLDARTTSADETRQLGIEVGDFVSLDPRVEVTDTGFIRSRFLDDKASVAC
                     IVGACKALLDANLRPSQRLTILISINEEIGHGASTGIPHDAKELLVVDMAAVGPGQNS
                     DEFSVGICAKDREGPYHLEMRRNLMALAQSAGIPYKVDIYPYYGSDGKSALLAGGDLM
                     VGLVGPGVEASHSYERTHKDSLIATTRLLVEYMLS"
     gene            complement(1821..4169)
                     /locus_tag="VFF70_06595"
     CDS             complement(1821..4169)
                     /locus_tag="VFF70_06595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44401.1"
                     /translation="MKASSPLRPLQLLLVSTLLLIFAAQTTLGSSSNSAALDEEYHLG
                     AGYAYLRTGDPRLSTEHPPLINVWNALPLTFLDPKLPLESEAWQNAATDDFGDQFLWQ
                     ANYDHAVPIILLGRLPILFLGLLLGAIIFRFANDLFGVNAALLALTLFAFDPNLIAQS
                     RLSTTDLGLALTMTLALWRMWSWLEKPTRRNLIILGVVTGTALMTKFTGLLLAPMFVC
                     IALLHPLKPVQTYGRGIVRRVLSLMLVGIISLLVIWIVYGFEIRGGLPAATYWQGLVK
                     IYTEYSQGYPTFLFGQISRTGWWYYFPITFLLKTPLPTLLLCFIGVVLTLKQRMLRQT
                     SVAWLPAALSMTAALFSPLAIGYRHILPILPFAIILAGQAANITFAKLNVRTGYQLRM
                     AGSVILIGWIAIGTLSISPHHLSYFNELIGGPAKSDNVLVDSNLDWGQDLPALKDWMA
                     HNKIDRVNLAYFGTALPAAYGINYWPMPAFLHFIIGPELDSYNPYTPEPGWYAISATS
                     LRMGLVYREQDLYAFFHDKIPDARAGYSILLYKIEYPTDTPIDRSVIVGTPTYAFSPE
                     QLGVTPGHRLVVKWADNPDAMIFAMNGSARYFLADRFSFSKDFHDALYSAGQIDHGTL
                     NVDARSVVEAWLSQWQSDILHLTDRTVLNWPINFADKLELIGVHLKATEISPDGNVDL
                     TTYWRVIGDLDPSLQMFVHVLDQHEKLVAQYDGWQTAIRGLEISDVIAQRIHISLPVD
                     TSPGQYSLRLGLYNSDSSIRFSIRLPIGLARDQVVLPSITIR"
     gene            complement(4252..>4842)
                     /locus_tag="VFF70_06600"
     CDS             complement(4252..>4842)
                     /locus_tag="VFF70_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HZY44402.1"
                     /translation="RNQKIVFQEDNTTDRAMLDRLGLDEFDQVIVLSYSDTLGQQEAD
                     ARTLITLLHLRDMGDKGHYDSRVVSEMLDVRNRELAEVTKADDFIVSNKLTSLMLSQV
                     SENKELNAVFADMFDPEGSEIYLKPASDYIDLGKSINFYTVVEAARSRGHVALGYKLA
                     GHMGNAAQAYGVVVNPDKSSKVTFDQDDRIVVVAED"
BASE COUNT         1147 a         1307 c         1284 g         1104 t
ORIGIN      
        1 gctttaaccg gaatatccgg caccggctcg ttcagcggat cgaaccaatc ggctttattc
       61 aagcgccaat tatccatcaa tgtgcgatga tgtgaatcgc gtgtcttgag catccatgtc
      121 tcgcagttct tgacagcatc gctctcgaag aacccgtccg gcagccggtt gaattcatcg
      181 gtctgataac gatacaccca cgtggtcgtg ggactatact tggcaatatc gtcggcgatg
      241 ttctcattga cggagtatac gatcccgatt ggcgagcctg cctcagcgca ctttttcagc
      301 cagtcgctga agccatcacg cttgccgctg gtaatgtgca cgctcaattt gctcatgctg
      361 cctccatgct tgaagtccag ctaaccttgc gaagctcagg gtcgaatgac aattttccag
      421 ctcaaccctc gacagtgtta tcaaaaaaac acgagccgct aaaatctagc ggctcgtgtc
      481 tgattacgac agcatgtatt cgactagcaa tcgcgttgtg gcgatgagcg agtctttgtg
      541 cgtgcgttca taactatgac tggcctcaac gcccggccca accagcccca ccattaaatc
      601 gcccccagcc agcagcgccg acttgccatc tgagccgtag taagggtaga tgtccacttt
      661 gtacggaata ccggcgctct gcgccagcgc catcaggttg cgccgcattt ccaaatgata
      721 cgggccttcg cgatctttcg cgcagatacc gacagagaac tcatcgctgt tttgacccgg
      781 cccgacagcc gccatatcca ctaccagcaa ttccttggcg tcatgcggga tacccgtcga
      841 agcgccgtgc ccgatttctt cattgatgct gatcaaaatc gtcaggcgtt ggctggggcg
      901 caagttcgca tcgagcagcg ctttgcacgc gccgacaatg cacgccacgc tggctttatc
      961 atccaggaag cgcgatcgga tgaagccggt gtccgtcact tctactcgcg gatcaagcga
     1021 gacaaaatcg ccgacttcaa tgcccaactg gcgcgtctca tcggccgaag tagttcgtgc
     1081 atcgaggcgg atttccattg tatcgtcggt gcgcggctgg ccttcgacga catcgcgcgt
     1141 atgcacatgg actgaagcct tctgcgcttg aagcgtgccc cgatagactt tgccgctctc
     1201 ggtggagatc gaaacgcctt caccttcagc tgccgtccag gccaatccgc caatgcggtt
     1261 catgcggacg cgtccgttcg gcaggatttc tttcaccatg ccgcctaacg tatccacatg
     1321 cgccgtcatg gcgcgcggcc ggctggaagc cttgccttgc caggttgcaa tcagtgagcc
     1381 tttggtgttg aagcgcgtat ccagcttcaa atttgccatg gtctctcgca caaagtgcga
     1441 cgcctgatgt gtgttgccgg tcgggctggg gatattgagt aaatcggtca ggaaggacgt
     1501 aatgaagcgg acatcaatat cgatactgcg cccacccgac ttgacagaag tagcgactgt
     1561 ctggccctga tttgcctttt ttgcgggtga cttcttaggt gttgccttct ttatagccac
     1621 attatcctcg caatctttat gtactaaact atgtccgata aaatcgctga ccaatattgt
     1681 caagcgacaa gggcgattat agcacaagca tgatcgaata ttcaagttac agttcacatg
     1741 cggctcagca acgtagtgct gaccgtcgat acagaacccg attggttggt gcggctcgat
     1801 tggactttta cgctgaaaaa ttagcggatc gtgatcgaag gaagaacgac ttgatctctt
     1861 gccaggccga tcgggagacg aatggagaag cgtatcgatg agtcggagtt gtacagacct
     1921 aagcgcagag agtattggcc gggggaagta tcgacaggca gtgaaatgtg gatgcgttgc
     1981 gcgatcacat cgctgatttc caatccgcgg atggccgtct gccagccgtc gtattgggcc
     2041 acaagctttt cgtgttgatc aagaacatgc acaaacattt gcaaagatgg atcgaggtcg
     2101 ccgatcaccc gccagtacgt ggttagatcg acattgccgt ccggtgaaat ctcggttgct
     2161 ttgagatgca caccaatcaa ttctaatttg tcggcgaaat tgatcggcca gttgagcaca
     2221 gtccgatccg ttaagtgcaa tatgtcactc tgccattgcg acagccacgc ttcaaccacc
     2281 gatcgcgcgt ccacgttcaa cgtgccatga tcgatttgtc cggccgaata cagggcgtca
     2341 tgaaaatctt tgctgaatga aaagcgatct gccaggaagt aacgcgctga gccgttcatg
     2401 gcgaagatca tcgcgtcggg attgtcggcc cacttcacca cgaggcgatg tcccggtgtc
     2461 acaccaagtt gctctggcga aaaggcgtac gtcggcgtgc cgacgatcac cgaccgatcg
     2521 attggcgtgt ccgttgggta ctcaatcttg tacaatagaa tcgagtaacc cgcgcgcgca
     2581 tccggtatct tatcgtggaa gaaagcatac agatcttgct cacgatacac caagcccatt
     2641 cgcaaacttg tggcactgat cgcgtaccag cccggctccg gtgtatacgg attatacgaa
     2701 tccaactctg gcccgataat gaaatgcaag aatgctggca tcggccaata gttgatgcca
     2761 tacgcggcgg gcagcgcggt accgaaatac gccagattca cgcgatcgat tttgttgtgt
     2821 gccatccaat ctttaagcgc aggtaagtct tggccccaat ccaaatttga atcgaccaaa
     2881 acattatccg acttggccgg tccgccgatc aattcattga agtaggagag atgatgagga
     2941 gagatcgata acgtgccaat cgcgatccaa ccgatcaaaa ttacgctacc cgccatccgc
     3001 agttggtaac cggttcgcac gttgagttta gcgaatgtga tgttcgctgc ttgcccggcc
     3061 aagatgatcg cgaatggcaa tattggcaga atgtgtcgat agccgatcgc cagtgggctg
     3121 aataaggcgg ccgtcatcga taaagcagct ggcagccaag cgacggatgt ctggcgcagc
     3181 attcgctgct taagagtcag caccacgccg atgaaacaca acaacaacgt cggcagggga
     3241 gtcttgagca agaacgtgat cgggaagtaa taccaccacc cggtgcggct gatttgtccg
     3301 aacaaaaacg taggataccc ttgcgagtat tcggtataga ttttgaccaa gccctgccag
     3361 taagttgcgg ccggtaagcc acctcgaatc tcaaagccat acacgatcca aatgacgagc
     3421 aggctaatta ttccaacaag catcaagctc aacactcgac ggacgattcc gcgtccatag
     3481 gtttgaacag gctttagcgg gtgtagcaat gcgatacaga caaacatcgg cgcgaggagc
     3541 aggccggtaa acttcgtcat gagtgccgtg ccggtgacca ctcccagaat gatcagattg
     3601 cgccgcgttg gtttctccag ccatgaccac atgcgccaca acgccagcgt cattgtcagc
     3661 gccaagccga gatcggtcgt tgagagtctt gactgcgcaa tgagattcgg atcgaacgcg
     3721 aagagcgtta acgcgagtaa tgccgcgttc acgccgaata gatcgttggc aaagcggaag
     3781 atgatcgcgc ccagcaacag ccctaaaaac agaatgggca atcggccaag caaaataatc
     3841 gggactgcgt gatcgtaatt tgcttgccac aggaattgat caccaaagtc atccgtcgcc
     3901 gcgttttgcc acgcctcact ctcgagcggg agtttgggat caagaaacgt cagcggcagt
     3961 gcgttccata cattgatcag cggcgggtgt tcggttgaca ggcgcggatc gccagtgcgc
     4021 agataagcat accccgcgcc gagatgatat tcttcgtcca atgcggcgga atttgacgat
     4081 gatcctaaag tcgtttgcgc cgcgaagatc aacaacagag ttgaaacgag aagcagctgc
     4141 agcggacgga gtggactgga tgctttcacg aatggttatt aagcgcaaag ttccgagccc
     4201 tctcctgagg cggttattat tttccgcttc aggagagggc agtgggtgag gctaatcttc
     4261 cgctacaacc acaattcgat cgtcttgatc gaatgtgacc ttgctcgatt tatcaggatt
     4321 aacgacgacg ccgtaggctt gggctgcgtt gcccatatgc ccggcgagct tatagcccaa
     4381 cgccacatgt ccgcgactgc gcgccgcttc gaccacggta tagaagttga tcgacttgcc
     4441 gagatcgata tagtcgctgg ccggcttcaa gtaaatttcc gagccttccg gatcaaacat
     4501 atcggcgaag accgcattga gttccttgtt ctcagacacc tggctcaaca tcaggctggt
     4561 gagtttatta ctgacgatga agtcatccgc tttggtgact tcggccaact cgcgattgcg
     4621 tacgtccagc atttcgctga cgacgcggga atcatagtgg cccttatcgc ccatatcgcg
     4681 cagatgcagc agcgtgatca aagtgcgcgc atcggcttcc tgctggccga gtgtgtctga
     4741 atacgacaag acgataactt gatcgaactc gtcgaggccg agtctgtcca gcatcgcgcg
     4801 atcagtcgta ttgtcttcct ggaagacaat tttctggttg cg
//
LOCUS       DASRTJ010000323         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_273029,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000323 DASRTJ010000000
VERSION     DASRTJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273029"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFF70_06605"
     CDS             <1..689
                     /locus_tag="VFF70_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HZY44403.1"
                     /translation="MQWWALGRVDELMRYSDATWRKMKASGLKMVFSGAESGSDEVLA
                     RMNKGGKVTANLTIELAKRLKSYNIVPEFSFVMGNPPDPVTDINATIQFIRKIKEVNS
                     ETETILYVYTPVPQEGTLYEEARALGFKFPDTLDQWVNGDWRSFSLRRDPHTPWLDPK
                     IKRTVRDFERVLNAYYPTVTDSKLTGVKRAVLRSMGAWRYHLQFYSNPIELRAFHKLL
                     GYQRPETTGF"
     gene            692..1936
                     /locus_tag="VFF70_06610"
     CDS             692..1936
                     /locus_tag="VFF70_06610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZY44404.1"
                     /translation="MRTVVQRFSLRWHRASLIVLLLVVCLIFAVHTFWPARSATVHGF
                     PVYYTAGWLFANGQWGPNAYDDIWFGDRVEFLTQNGVREIYAPSPPLLTVFFALFAGL
                     DIAAARDVWAWLNVLMLMISLGLIIGALARFRGSAWRVASIGFALLAAPTREHFHLGQ
                     VYILLLLFFALAFWLAQHQHQIFAGAALGVALVTKLSGLPLLFTLLAQRQWRLSVSVM
                     ITAGSLGVGGLLLMGLPTWQAFAQTLIDRLTNYPIIAVTAFQSTSSLFHHLFVYDAQW
                     SPQPIWSQPWLAPLLTWSIFFGAVIITMRRARHSVLDVAFAAGTTLSVILLPLAEEYH
                     YVLLLLPLAVMMARVLQTPYSLRNLIWPTIIIFLIGWPLPYTDPELTRGWLSVLAYPR
                     LYGGWLLWLWHIRRMNVEKTSP"
     gene            1894..>2969
                     /locus_tag="VFF70_06615"
     CDS             1894..>2969
                     /locus_tag="VFF70_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44405.1"
                     /translation="MAHSKNERGKNKPVIRSILSRRFRRRLILIAANIANGLLLPLLN
                     PIVSLLVVWLASIDLWGEYVALLIGVQLAAHIIGWGNKDYLLRQFSFNPTRLTELWRG
                     SLITRGLLLLPFGLLILWLEPARAVWLLVWCAVLVFDQAFDVWVTYRKDFVFSSGVEL
                     FGLIVTLAGIGLVSARLDVDALIVISIGSTLIKALIYLLRYRSSALAPSSEQPGQPIE
                     WAYFRRAFAFFALGFSGLLQSRIDLYIVGLVLSKNSIGQYQVFITFLLYLQSLSAFVL
                     GPFVKNLYRLSYRLILNLSLRLLALGVLFCLPALILLDPVLHQFYKFDLPPIFFVLGG
                     VIALPSYFYLPIIYGLYKSGLTGRV"
BASE COUNT          542 a          761 c          836 g          830 t
ORIGIN      
        1 agatgcaatg gtgggcgttg ggccgcgtcg atgagttgat gcgctacagc gatgccacct
       61 ggcgcaagat gaaagccagc ggcttgaaga tggtcttcag cggcgcggaa tccggatcgg
      121 atgaagtctt ggcgcgcatg aacaaaggcg gcaaggtcac ggccaacctg acgatcgaat
      181 tagccaagcg cttgaaaagc tacaacatcg tgccggagtt ttcattcgtg atgggcaacc
      241 cacccgatcc ggtgacggac atcaatgcca cgatccaatt cattcgcaag atcaaagaag
      301 tgaattctga gaccgaaacg atcttgtatg tgtacacgcc cgttccgcag gaaggcacgt
      361 tgtatgaaga agcccgcgcg ctcggtttca aattccccga cacgctcgat caatgggtca
      421 atggcgattg gcgcagtttc tcgctacgtc gcgatccgca cacgccatgg ctcgatccta
      481 aaatcaaacg caccgtgcgc gattttgaac gggtattgaa tgcctactat ccaacggtga
      541 ccgattccaa acttactggc gtcaaacgtg ccgtcctgcg atcgatgggt gcatggcgtt
      601 atcatcttca gttctattct aatccgatcg agctccgcgc cttccacaaa ctgctgggct
      661 atcagcggcc cgaaaccact ggattctaaa catgcggacg gtcgtgcagc gtttcagcct
      721 gcgctggcac agggctagcc tgatcgtttt gttgttagtg gtctgcctaa tttttgcagt
      781 gcacacgttt tggccggcac gctccgccac ggtgcatggt ttccctgttt attacacagc
      841 gggttggctg tttgcgaatg ggcagtgggg accgaatgct tacgatgaca tctggtttgg
      901 cgatcgagta gagtttttga cgcagaacgg cgtgcgggaa atctatgcgc caagtccgcc
      961 gctcctgacg gtttttttcg cattgtttgc cggtctggat atagctgcag cgcgcgatgt
     1021 ctgggcctgg ctcaacgtgc tgatgttgat gatcagcctg ggtctgatca tcggtgcgct
     1081 ggcccggttt cgcgggtcgg cgtggcgtgt ggcgagcatc ggctttgcgt tgctggctgc
     1141 accaacgcgt gaacattttc acttggggca agtctatatt ctgctgctgc tgttttttgc
     1201 gttagcattt tggttggctc aacatcaaca tcaaattttc gcaggcgctg cattgggcgt
     1261 tgcgctcgtt accaagctaa gcggcttgcc actgttgttc acattgctgg cccagcgtca
     1321 atggcgtttg agtgtcagcg tcatgattac tgcggggtcg ttaggcgtgg gcgggctgct
     1381 gctcatgggg ttgccaacgt ggcaggcctt cgcgcaaaca ctgatcgatc gtttgacgaa
     1441 ttacccgatt attgccgtga ccgcctttca gtcaacgtcg agtctctttc atcacctgtt
     1501 tgtctatgac gcccaatgga gtccgcaacc gatctggtca caaccgtggt tggctccact
     1561 gctcacttgg tcgatctttt tcggagcagt gatcatcacg atgcggcgcg ccaggcattc
     1621 ggttctcgac gtggcctttg ccgcgggaac gacattgagt gtgatcttgt tgcccttagc
     1681 cgaagaatat cactacgtat tactactctt gccgctcgct gtgatgatgg cccgtgtctt
     1741 gcagactcct tattcattga ggaatctcat ctggccaacc attatcatct tcttgatcgg
     1801 ttggccgttg ccatatactg atcctgaact gacacgcggc tggctgtcag tgttggcgta
     1861 tccgcgcttg tatggcggtt ggttgttgtg gctatggcac attcgaagaa tgaacgtgga
     1921 aaaaacaagc ccgtgattcg atcgatctta tcgcgcagat tccgccgacg cttgatcttg
     1981 atcgcagcca acatcgccaa tggcttgttg ctgccgctgc tcaatccgat cgtttcactc
     2041 ttggttgtgt ggttagcctc gatcgatctg tggggggagt acgtcgcgct tttgattgga
     2101 gtgcagttag ctgcacacat cattggctgg ggcaacaaag attatttgct gcggcaattt
     2161 agtttcaatc caacgcggct gaccgaattg tggcgcggca gtttgattac gcgcgggttg
     2221 ttgctgttgc cgttcggctt gttgatcctg tggctcgaac cggcgcgtgc ggtgtggttg
     2281 ctggtttggt gcgcggtctt ggtcttcgat caagcatttg acgtgtgggt cacttaccgg
     2341 aaagactttg tgttttcaag tggtgtcgag ctgttcggtt tgatcgtgac gctagcgggc
     2401 attggtttag tgagtgcgcg gctggatgtc gatgcgctga tcgtgatctc gatcggatcg
     2461 acgttgatca aagcgttgat ctacttgttg cgctatcgat cctcggcgct ggctccgtca
     2521 tctgagcagc ccggccagcc gatcgagtgg gcgtacttcc ggcgggcgtt cgccttcttc
     2581 gcgctcggtt tttccggttt gctgcaatca cgcatcgatc tctatattgt tggcctcgtg
     2641 ctatcgaaaa atagtatcgg tcaatatcaa gtgttcatca ccttcttgct gtatctgcaa
     2701 tcgctgtcgg ccttcgtgct cggaccattc gtcaagaatc tctaccggtt gagttatcgc
     2761 ttgatcctaa atctctcctt gcgcttgctg gcgttaggtg tgctgttctg tctgcccgcc
     2821 ctcattctgc tcgatccggt gctgcatcaa ttctacaaat tcgatctacc gccgatcttt
     2881 tttgtattgg gcggtgtcat cgcgctgccc agttattttt atttgccgat aatctacggc
     2941 ttgtacaaga gcggtctgac cggacgtgt
//
LOCUS       DASRTJ010000324         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_18703,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000324 DASRTJ010000000
VERSION     DASRTJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18703"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFF70_06620"
     CDS             complement(<1..848)
                     /locus_tag="VFF70_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZY44406.1"
                     /translation="MSLRTRLLLSYTLVIVTCLAVIGIALLLLLRDDPVQKRLLTGRL
                     TTEAGVVARLVRNQFLNGATTDQILRRLETVTSGNSDTRILVINSDSGAVLADTDNSL
                     TGKNLFELGKPQRSGNVINGAFDADNQHWLYSSRQFLLAPITRVELIAAAPFDKTPLL
                     QDPVFSELFRPLFIAGLLALGISIVLAVIITRSVTRPMQHVAQAADRIAGGDYNQRVP
                     VEGSSEVKEMATNFNAMAQNVRDTQQSQRDFLANVSHELKTPLTSIQGFSQAIRVELS
                     TDDDLVA"
     gene            complement(956..1504)
                     /locus_tag="VFF70_06625"
     CDS             complement(956..1504)
                     /locus_tag="VFF70_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HZY44407.1"
                     /translation="MQIQFECTLDDYIAFHRYCFDNVSSIKRNYRIGLFGLALVLMTL
                     GLLDFSRGRSVTGAIWFTAGLLWPVLYPMYYRRRLGQLAKHSLKASPNRGTLGPHTIS
                     ISENELHETTDVNDSRWTWLGIERIEQDNQCIYIFVGTTQAHVISKRSFANDEEAVKF
                     YNTAKKYFEKGRASNHPQDHAL"
     gene            complement(1572..>1798)
                     /locus_tag="VFF70_06630"
     CDS             complement(1572..>1798)
                     /locus_tag="VFF70_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZY44408.1"
                     /translation="FKVLLDKWQLPPDEVVMVGDMLGADILGAHNANMRGIWATMQAD
                     RASNDAHSDTIIPDAKIDALAKLPGVLGRW"
BASE COUNT          465 a          457 c          446 g          430 t
ORIGIN      
        1 gctactagat cgtcgtctgt tgataactcg actcggatcg cttgggaaaa accttggatg
       61 ctggtcagcg gcgtcttgag ttcgtgcgac acattcgcca agaagtcacg ctggctctgc
      121 tgcgtgtcgc gcacattttg cgccatcgca ttgaagttcg tcgccatctc tttgacttcg
      181 ctcgatcctt cgacgggcac acgttgatta taatcgccgc ccgcaattcg atcggcggcc
      241 tgagcgacgt gctgcatcgg ccgcgtcacc gatcgcgtga tgatcacggc taacacgatc
      301 gagataccca gcgccaacaa tccggcgatg aataaaggcc ggaacaattc actaaagacc
      361 gggtcttgaa gtagtggcgt cttatcgaac ggcgcagcgg cgatgagttc aacgcgagtg
      421 atcggcgcga gcagaaattg tcgcgatgaa tacagccaat gttgattgtc cgcatcgaac
      481 gcaccgttga tcacattacc cgatcgctgc ggcttgccga gttcgaataa atttttcccg
      541 gtcaacgaat tatcggtatc ggccaacacc gcgccggaat ccgagttgat gaccaggatg
      601 cgcgtatcac tgttgcccga agtgactgtc tccaggcggc ggagaatttg atccgtcgtt
      661 gcgccattca agaattgatt gcgcaccaaa cgcgcgacga cgcccgcctc ggtcgtcaag
      721 cgtccggtta acaaacgctt ctgaaccgga tcgtcgcgca ggagcaacag caacgcgatg
      781 ccaatgaccg cgaggcaggt cacgatgacg agcgtgtaac tgagtaacag gcgagtacga
      841 agagacatga ttagtgaata gtaattagtg ctgggtggtg catactgcct tcattataag
      901 cgggcgatgt aaacgcggtg taaattctcg gttcaggttg tgtaaaagcg ataatctaca
      961 gggcgtgatc ctgtgggtgg ttagatgctc ttcctttttc aaagtatttt ttagcagtat
     1021 tgtaaaactt aaccgcctct tcgtcattgg caaaagatcg tttggaaata acatgtgctt
     1081 gggtagttcc aacaaaaata tagatgcatt gattgtcttg ctcaatacgc tcaatgccca
     1141 accaagtcca gcgtgaatca ttcacatctg tagtctcatg tagctcattt tcgctgatag
     1201 atatggtgtg cggaccgagt gttccacgat tcgggctagc ctttagggag tgcttcgcta
     1261 attggccgag cctgcgtctg taatacatcg gatacaaaac aggccacaat aaacctgcag
     1321 tgaaccaaat tgcgccggta actgatcttc cacgactaaa atccaataag ccaagagtca
     1381 tcaataccaa tgcgaggccg aacaatccaa ttcgataatt gcgcttgatc gatgatacgt
     1441 tatcaaaaca atatcgatga aaagcaatat aatcatccag tgtgcactca aattgtattt
     1501 gcatcagcca tagctccaag aaacagattg ttgcacaacg aagtgaattc aattcgtcta
     1561 ataaagatca atcaccatcg tcccaacacc cccggcaact ttgccagcgc atcgatcttc
     1621 gcatcaggaa tgatggtatc cgaatgagca tcgttcgatg cgcgatcggc ttgcatggtg
     1681 gcccagatgc cgcgcatatt ggcgttgtgc gcgccgagaa tatccgcgcc aagcatgtcg
     1741 ccgaccatga cgacttcatc cggcggcaac tgccacttat ctaacagcac tttaaaga
//
LOCUS       DASRTJ010000325         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321525,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000325 DASRTJ010000000
VERSION     DASRTJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321525"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFF70_06635"
     CDS             complement(<1..403)
                     /locus_tag="VFF70_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY44409.1"
                     /translation="MDFNLTADQKMFRDLFRDFATKEVAKVAKHVDETEEPPIDLLKK
                     AAAQGFLAAAIPEEFGGAALDPLSYALLIEEIAKVCASTAITLALHNSLSVMPLILFG
                     SDDQKQRWAPKFAESIGAFALTEPESGTDANH"
     gene            complement(504..1292)
                     /locus_tag="VFF70_06640"
     CDS             complement(504..1292)
                     /locus_tag="VFF70_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY44410.1"
                     /translation="MADFIRYTVEDKVATLVIDHPPVNALNKQTLTELDAALEALLAD
                     VTVKIIVITGGGQMAFVAGADVGDIGEIVKTKDAAAAQAMIELGQRVFNKIEGSNKPV
                     IAAINGFCLGGGLELAMACHIRIAGDRSRFGQPEINLGIMPGWGGTQRLTRIVGPSKA
                     TEIMLTGDQITAQQAMQLRLVNMVVPGGEVMRQSIGLAKKFANKSSVGIAAILGSIRE
                     GLDADLQSGLAAERKNFVNVASSADAQEGIMAFLEKREAKFQDK"
     gene            complement(1411..1755)
                     /locus_tag="VFF70_06645"
     CDS             complement(1411..1755)
                     /locus_tag="VFF70_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZY44411.1"
                     /translation="MIAEFNGGKKLSEFTPDRLMYQLINEAARAAEEDIADVRDIDMA
                     MIAGTGMMVKGERIGPLALADKLGIDVVVKGLEDFEKKFGKRFHPANRLYELVKENRL
                     GEKSRAGFLEYV"
     gene            complement(1801..2625)
                     /locus_tag="VFF70_06650"
                     /pseudo
     CDS             complement(1801..2625)
                     /locus_tag="VFF70_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
     gene            complement(2635..3843)
                     /locus_tag="VFF70_06655"
     CDS             complement(2635..3843)
                     /locus_tag="VFF70_06655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZY44412.1"
                     /translation="MKDVVIVDGVRTPVGNHGGALRTIGARELLAIVFRGVIDRLKID
                     PASIDEVIAGCIAQPSDAPNITRVSALMAGVPKEVPALTVARNCNSGMDAIIEAWRRI
                     QLDEGSIYLVGGVESMSNIPYILKDARWGIKLRHSQLTDALWEGLTDPICGQLMGRTA
                     ENLVEEFEITRLEQDEFAVQSHKKAFMATRMNKFKDEIVPVEIIKKIAGQEVAKEQIT
                     QDETINAGLTVQKAALYPTVFKEGGTVTPVNSCGISDGASAMIIMSADKAKELGYKPI
                     ARILGYGFAGVDPSRMGIAPAYAVPKALDKAQLKLSDMDLIEINEAFAAQVIAVGRTL
                     ADRGWDWTKVNVNGGAIALGHPVGSSGCRIVVTLIHEMQRRGSKYGLATLCAAGGQGA
                     ALVVERVNGQ"
     gene            complement(3928..>4402)
                     /locus_tag="VFF70_06660"
     CDS             complement(3928..>4402)
                     /locus_tag="VFF70_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HZY44413.1"
                     /translation="LEGLEQLVTYLEAAYAPDQKLAARTRLRRIANAYLEFFQNNPDY
                     YRLMMSFDRGHFQETVDPKVYQQVYHRSLRGLEFLVLAVEQAKAEGVLDVDDTRHAAS
                     VLWAGLNGVLVLLSHPLRADIVASDLESMYQSMIDVLIKGLKKHRVKSEAGKQAA"
BASE COUNT          929 a         1363 c         1059 g         1051 t
ORIGIN      
        1 aatgatttgc atcggtgccc gactcgggtt cggtcaacgc aaacgcgccg atcgattctg
       61 cgaactttgg cgcccaacgc tgcttttgat catcgctacc gaataagatc aggggcatga
      121 ctgagagact attgtgcaaa gccaaagtaa tcgccgtcga cgcgcagact ttagcaatct
      181 cttcgatcag cagcgcatag ctcagcgggt ccagcgctgc gccgccgaat tcttccggaa
      241 tcgccgccgc caaaaatccc tgcgcggctg ctttcttcag tagatcgatc ggcggctctt
      301 cagtttcatc gacatgcttc gccacctttg ccacttcttt cgtcgcgaag tcacggaata
      361 gatcgcggaa cattttttga tcggcggtta aattgaagtc catatgaaac tccttgaccg
      421 aagaccgcag accgaagacc gaagaccgca gttaacgacg atcaacaatg attcatctgc
      481 tatcttcggt catcggtcgg cagtcatttg tcttggaact tcgcctcgcg cttttccaag
      541 aacgccatga taccttcttg cgcatccgcg ctcgaagcga cgttgacaaa gttcttgcgt
      601 tccgcggcca agcccgattg caaatccgca tcaagccctt cgcggatcga tccgaggatc
      661 gccgcgatgc cgaccgagct cttattcgcg aacttcttcg ccaacccgat cgattgacgc
      721 atcacttcgc cgcccggcac gaccatgttc accaagcgca gctgcatcgc ttgctgcgca
      781 gtgatttgat cgccggtcag catgatctcg gtggctttgc tcggcccgac aatacgcgtc
      841 agtcgctgcg taccgcccca gcccggcatg atgccgaggt tgatctccgg ttggccgaag
      901 cgcgatcgat cgcccgcgat gcgaatatgg cacgccatcg ccagttccaa tccgccgccg
      961 agacagaaac cattgatcgc ggcgatcacc ggcttattcg agccttcgat cttattgaag
     1021 acacgttgcc ccaattcgat catcgcttgc gcggcagccg catctttggt tttgacgatc
     1081 tcgccgatgt cgcccacgtc cgcgcccgca acgaacgcca tttgtccgcc gcccgtgatc
     1141 acaattattt tcacggttac atcggcaagc aacgcctcca gcgccgcgtc aagttcagtc
     1201 agcgtctgct tgttcagcgc attcacgggc ggatgatcga tcaccagcgt cgcaactttg
     1261 tcttcaacgg tgtaacgaat aaagtctgcc atgtgtcttt tccttttatc gcgtcattgc
     1321 gaggaggtca ctaggccggc gaagcaatct ctttcgacta tcaatgagat tgttccgccg
     1381 ctttgcggct cgcaatgacg cgtggttgaa ttagacgtac tcaagaaatc ccgctcgact
     1441 cttctcaccc aatcgattct ccttcaccaa ttcgtacaat cgattcgccg gatggaagcg
     1501 cttgccgaat ttcttttcga aatcttcaag acctttgacg acgacgtcga tacccaattt
     1561 atccgccagc gccagcggtc caatgcgttc gcctttgacc atcatacccg tgccggcgat
     1621 catcgccata tcgatgtcgc gtacatcggc gatgtcttct tcggcagcgc gcgcggcttc
     1681 gttgatcagt tggtacatca atcgatcagg cgtgaactcg ctcaacttct tgccgccatt
     1741 aaattcggcg atcattttct tcacggcttc atctgtattg tcaccgtaac cggaaaagcc
     1801 cgcgcccgat ttttcgccga gccgaccggc ctgcaccaat ttctcaaaca acatcgcggc
     1861 ttccattcga tcgccgtact gcgaatacag atattgaccc acgtcatagc acacatcaag
     1921 gccgagaaag tccatcagcg tgaacggacc catcggccaa ccgtaatcga ccatcgcact
     1981 atcgatctct tgcgcggtcg ccgcgccgtc ttgcagacac agcaccgctt cgttgagata
     2041 cggcatcaat aatcgattga ctaagaagcc cggacattct ttcaccacga ccggaatctt
     2101 tctcaacgat tcggtaaacg tcactaaatc ctgcacgctt tcttcatcgg tttctgcacc
     2161 gcgaatgatc tcgaccaact tcatcacatt cgccggattg aagaagtgca tgccgatcat
     2221 cttatgcggg cgtttcgtcg cgcggcccat ctccgtaatt gacagcgccg atgtgttcga
     2281 ggcaaagagt gtgccttcgg gacaaatggt atcgagctca gaaaaaattt actgcttcaa
     2341 ttccattttt tccggcaccg cttcgatcac aatatcgaca tcggcaaagt ctgcatcact
     2401 cagggacggg atgatcagat cgagtttgcc ctgcatctcg ctggcgctca tcttgccttt
     2461 atcgacgcgc gtttgataga tcgatcggat ttgctccatc ccgcgatcga gcatcttctg
     2521 atcgacatcc ttcaacacga cgggcaaacc cgaaaacgaa atcacctgcg cgatgcccgc
     2581 gcccatcgca cctgcaccga taacgcccgc tttgaaaatg tacatgcgat cctcctattg
     2641 tccgttgact cgttcaacca ccagcgccgc gccctgcccg ccggccgcgc acaaagtggc
     2701 gagtccatat ttcgatccgc gccgctgcat ttcgtggatc aacgtgacga cgatgcgaca
     2761 gccgctcgat ccgacgggat ggccaagcgc gattgcgccg ccgttgacat tgactttggt
     2821 ccaatcccaa ccccgatcgg ccagtgtgcg cccgacggcg atcacttgcg cggcgaacgc
     2881 ttcattgatc tcgatcaagt ccatgtcact taatttcaac tgcgctttgt ccaacgcctt
     2941 cggcaccgca taagccggag cgatacccat gcgcgacgga tccactcccg cgaagccgta
     3001 accgagaatg cgcgcgatcg gtttgtagcc caactctttc gctttatccg ccgacatgat
     3061 gatcatcgcg ctcgcgccat cgctgatgcc gcaagaattc acgggcgtca ctgttccgcc
     3121 ctctttgaag accgtcggat ataacgccgc tttctgaacc gtcagcccgg cattgatcgt
     3181 ttcatcttgc gtgatttgtt ccttggccac ttcctgcccc gcgattttct tgatgatctc
     3241 aacgggcacg atctcgtctt taaatttatt catgcgcgtc gccatgaacg ccttcttgtg
     3301 cgactgcacg gcaaactcat cttgttctaa gcgcgtgatt tcaaattctt cgacgagatt
     3361 ttccgcagtg cggcccatca attgtccgca gatcggatcg gtcaggcctt cccacaacgc
     3421 gtcggtcaat tgcgagtgac gcagtttgat accccagcgc gcatctttca ggatgtatgg
     3481 aatgttgctc atgctctcga cgccgccgac caagtagatc gatccttcgt cgagttgaat
     3541 gcgccgccat gcttcgatga tcgcatccat gccgctgttg cagttgcgcg ccacagtgag
     3601 cgctggaact tctttcggca caccggccat caacgccgag acgcgtgtga tgttcggcgc
     3661 gtcggatggc tgcgcgatgc agcccgcaat cacttcatcg atcgaggccg gatcgatctt
     3721 caggcgatcg atcacgccgc gaaagacgat cgccagcaat tctctggccc cgattgtcct
     3781 cagcgcccca ccgtgattcc cgaccggcgt tctcactcca tcgacaatga cgacatcttt
     3841 catgttggtt ctcctatgac cgcagacgac cctatccgct tcgctactag ggcgggcaga
     3901 tggtcgaccg cagttgattc tttgatgtca ggctgcttgc ttaccagctt cactcttcac
     3961 tcgatgcttc ttcaagccct taatcaacac atcgatcatc gattgataca ttgattccaa
     4021 atcgctagcg acaatatcgg cgcgcagcgg atggctcaac aaaactagca caccattcag
     4081 tccggcccac aacacgctgg ccgcgtggcg cgtatcatcg acatcgagca cgccttctgc
     4141 tttggcttgc tcgacggcca gcaccaaaaa ttcaaggccc cgcagactgc gatgatagac
     4201 ctgctgatag accttaggat cgactgtctc ttggaagtgc cctcgatcga aggacatcat
     4261 caagcgatag tagtcggggt tgttctgaaa aaattcgagg taggcgtttg caatgcggcg
     4321 caggcgcgtg cgcgcggcca gtttttgatc gggcgcataa gcggcttcga gataggtgac
     4381 gagttgctca agtccctcta ac
//
LOCUS       DASRTJ010000326         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321742,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000326 DASRTJ010000000
VERSION     DASRTJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321742"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFF70_06665"
     CDS             <1..367
                     /locus_tag="VFF70_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HZY44414.1"
                     /translation="HIDRIDALNPVMIELSYEPMGEYGISGRRFFRKGGEVNRSHHVH
                     AYESTNPEVKKHLDFRDYLIAHSAEARQYGELKKELALKFPHDIEGYIEGKGAFVKEI
                     IRRAAEWREIQTYKTSDAL"
     gene            complement(410..1051)
                     /locus_tag="VFF70_06670"
     CDS             complement(410..1051)
                     /locus_tag="VFF70_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZY44415.1"
                     /translation="MLHYEINNSDVPIRLFKSDFLEYFTHISPITVLIIWVPVAGYFL
                     ARAVAEAAVGMNAIYIPIAFIIGLLVWTFTEYMMHRFVFHFRPRNAAQEKIIFLFHGV
                     HHAQPQLKTRLVMPPILSVPLAFLFYALFASIVGGLFNAPLWIAPMFSGVVTGYLMYD
                     MTHYATHHFAMHARWFKWLKRYHMMHHWKTPNERYGVSSPLWDVVFHTMPADK"
     gene            complement(1199..>1879)
                     /gene="hypE"
                     /locus_tag="VFF70_06675"
     CDS             complement(1199..>1879)
                     /gene="hypE"
                     /locus_tag="VFF70_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HZY44416.1"
                     /translation="ESMRVAAEEANIQIVAGDTKVVQKGKADGLYINTAGIGVVPIGI
                     EIGGSMAQPGDAVILSGPIGDHGIAVLGARGELDFEAEVCSDIAPLNHLIAAMLDASE
                     HIHVLRDPTRGGVATSLNEIAQQSNVCITLNEAAIPVRSAVRAACEMLGFDPLYVANE
                     GKLIAIVGREDADRVLQVMRQNRYGAEAVIVGEVNAQPQARVLMKTSIGSHRIVDVLM
                     GEMLPRIC"
BASE COUNT          479 a          537 c          465 g          398 t
ORIGIN      
        1 gcatatcgat cggatcgatg cgctcaatcc ggtcatgatc gaactcagtt acgaaccgat
       61 gggcgaatac ggtatttccg gccgcaggtt ttttcggaag ggcggcgagg tgaatcgatc
      121 gcatcacgtt cacgcctacg agtcgacgaa tccggaagtg aaaaagcatc ttgatttccg
      181 cgattacttg atcgcacatt ccgctgaagc ccggcaatat ggagaattga agaaagaatt
      241 ggcgctgaaa tttccgcacg acattgaagg ttacatcgag gggaaaggcg ccttcgtcaa
      301 agagatcatc cgtcgagccg ccgagtggcg cgagatccaa acctacaaaa cgagcgacgc
      361 actttagaag tgcgtcgctc gtttcattct tagcgtctgt atgcgagatt tatttatcag
      421 ccggcatcgt atggaacacc acatcccaca atggcgaact gacaccgtag cgctcgttcg
      481 gcgtcttcca gtgatgcatc atgtgataac gcttcaacca cttaaaccag cgcgcgtgca
      541 tggcgaagtg atgcgtggca tagtgcgtca tgtcgtacat caaatagccg gtcaccacgc
      601 cggagaacat tggcgcgatc cacagcggcg cattgaacag tccgccgacg atcgaggcga
      661 acaacgcata gaaaagaaac gccaacggca cactcagaat aggcggcatg accaagcggg
      721 tcttcaactg cggctgtgca tgatgcacac cgtgaaacag gaagatgatc ttttcctggg
      781 ctgcattgcg cggccggaag tgaaacacaa agcgatgcat catgtattcc gtaaacgtcc
      841 acaccagcaa gccgatgatg aatgcgatcg gaatatagat agcattcatt ccaaccgccg
      901 cctcggccac cgcgcgcgct aggaaataac ccgccaccgg cacccaaatg atcagcaccg
      961 tgatcgggct gatgtgcgtg aagtattcca agaagtccga cttgaataac cggatcggaa
     1021 catcactatt gttgatttcg taatgcagca tagttgattt ggcctcccgt aaattggctg
     1081 ctaagttgtt gaatggtatg gactaggctt aattgtacca gatattaaaa agatgctcgg
     1141 gtcgcccgaa catctttaac gatacaccaa aatacacctg ctcgcaacgc gacactggct
     1201 agcagatgcg cggcagcatc tcgcccatca agacatcgac gatccggtgg ctgccgatcg
     1261 aagtcttcat caagacacgg gcctgcggct gagcattcac ttcaccgacg attacggctt
     1321 cagcgccata tcgattttga cgcatgacct gcaaaacccg atcggcgtcc tcgcgcccga
     1381 cgatcgcgat caacttgcct tcattcgcta catacaatgg atcgaagccc aacatctcgc
     1441 aagccgcccg caccgccgat cgaaccggaa tcgcggcttc gttgagtgta atacacacgt
     1501 tggattgttg agcgatctcg tttagacttg tcgccacccc acctcgcgtc ggatcacgca
     1561 acacgtgaat gtgctcactc gcatcgagca ttgccgcgat caaatgattg agcggcgcaa
     1621 tatcgctgca cacctcggcc tcaaaatcta actcgccgcg ggcaccgagc accgcaatac
     1681 catgatcgcc gatcgggccg gagaggatca cagcgtcacc cggctgcgcc atcgatccac
     1741 ctatttcaat tccaatcggc acaacaccaa tcccggcggt gttgatatac aacccatcgg
     1801 ccttgccctt ttgtacaacc ttcgtatcgc ccgcgacaat ttgaatgttt gcttcttctg
     1861 ctgcaacgcg catcgactc
//
LOCUS       DASRTJ010000327         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321813,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000327 DASRTJ010000000
VERSION     DASRTJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321813"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFF70_06680"
     CDS             complement(<1..445)
                     /locus_tag="VFF70_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44417.1"
                     /translation="MVIDQTLVDHFLQAIGLGIGVAIILRVLGRFTIARRIRFSILAG
                     VLMIGLYWIVAGSNVSGQETLLQIFIALAVLFFANAGLHLFDALVWELLFERRRQRAV
                     PRLVVDLFNFIALTIVALIVLSRVFGADLSGLLVTSTVLSAVIGLS"
     gene            complement(496..1218)
                     /locus_tag="VFF70_06685"
     CDS             complement(496..1218)
                     /locus_tag="VFF70_06685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZY44418.1"
                     /translation="MIPLRDMNPTRRLPIMTIILIAVNVIVFLYTNSLNARAQTRFFI
                     DYGLIPLKITATLNTTTALTFITSMFLHGGWLHIGGNMLYLWIFGNNIEDRLGIIRFT
                     LFYIVCGIIAGLAQVAIDPTAPIPQIGASGAIAGVLGGYIVLFPRARIQTLLILFYFI
                     RIVELSAVWVLGFWFVLQVINGVTSAGQGDVAFFAHIGGFIAGAILIKVFCIGTSGPI
                     DWLSRSPNPSIDQPRNPNRWWD"
     gene            complement(1215..2075)
                     /gene="folP"
                     /locus_tag="VFF70_06690"
     CDS             complement(1215..2075)
                     /gene="folP"
                     /locus_tag="VFF70_06690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZY44419.1"
                     /translation="MGILNVTPDSFSGDGMLTEKDWIAASIDRAREMIQSGAHLIDVG
                     GESTRPGSRTISIDEEIDRVIPVIEALTQHGLGPISIDTYKAEVARRALDAGAALIND
                     VWGLRRDPDMGALAAQRGVPIVLMHNRSKPEDAAFQAQLGPRYIGSQYDDLIPDIARE
                     LSECVASARSAGIQDHSIIIDPGIGFGKTTEQNLELLNRLNEFTALGYPILSGPSRKS
                     FIGYTLNAPPDQRSAGTAAAVAISIVRGADIVRVHDVRSMAQVAKMTDAIVRRQRTGE
                     PRFASAREKA"
BASE COUNT          563 a          673 c          619 g          425 t
ORIGIN      
        1 gcgacaggcc gatgaccgcc gataaaacag tcgaggtaac cagcaagccg ctgaggtccg
       61 cgccaaagac gcggctgagc acgatcagcg cgacgatcgt caatgcgatg aagttgaaca
      121 gatcgacaac caagcgggga acggcgcgct gccgccgccg ctcaaagagt aactcccaga
      181 cgagggcatc aaacagatgc aagccggcat tggcaaagaa caacaccgcc agtgcgataa
      241 aaatctgcag gagagtttcc tggccggaca cgttgctgcc cgcgacgatc caatacagac
      301 cgatcatcaa tacgccggcc aagatcgaaa aacggatgcg gcgcgcgatg gtaaagcgac
      361 ccagcacacg caggataatt gccacgccga tgcccaagcc gatggcttga aggaaatggt
      421 caactaaagt ttgatcgatc accatttcaa ctcatcatcc atctgtcatg tgtcattcgt
      481 caatgtccag tgtctttaat cccaccatcg attgggattg cgcggctgat cgatcgacgg
      541 attgggtgac cgggacaacc aatcgatcgg gccgctggtg ccgatgcaga aaaccttgat
      601 caagatcgca cccgcaataa atccgccaat gtgcgcgaag aacgccacgt cgccctggcc
      661 ggctgaagtc acaccgttga tcacttgcag cacgaaccaa aaacccagca cccacacggc
      721 cgataattcc acgatgcgga tgaaatagaa caggatcagc agcgtttgaa tccgcgcgcg
      781 cggaaacaaa acaatgtagc cgcccagcac accggcgatc gcaccgctgg caccgatctg
      841 cggaatgggc gcagtgggat caatggcaac ctgagcgagt ccggcaatga taccgcacac
      901 tatataaaac agcgtgaagc gaatgatgcc taatcgatct tcaatgttgt tgccgaagat
      961 ccataaatac aacatgttgc cgccgatgtg cagccagccg ccatgcagga acatggaggt
     1021 aatgaaggtc agcgcagttg tggtattcaa cgtggcggtt atcttcagcg gaatcaaacc
     1081 gtagtcaatg aagaaccggg tctgtgcgcg tgcgttcaag ctattggtat acagaaacac
     1141 aataacattg acggcgatca agatgattgt catgatcggc aggcgccgcg tcggattcat
     1201 gtcgcgtaat ggaatcatgc tttttccctg gcagacgcga accgcggctc gccggtgcgc
     1261 tggcgccgca caatcgcatc ggtcatcttc gctacttgcg ccatcgatcg gacatcatgc
     1321 acccgcacga tatccgcgcc gcgtacaata ctaatcgcca cggcagccgc cgtcccggcg
     1381 ctccgctgat cgggtggcgc gttcaacgtg taaccgataa acgatttgcg cgacggcccg
     1441 ctcaagatag gataaccgag agcggtaaat tcgttcagcc gattcaagag ttccaaattc
     1501 tgttcggtcg ttttgccgaa gccaatgccc ggatcaataa tgatcgagtg atcttgaatt
     1561 cccgccgagc gcgcactcgc cacacactca ctcaactcgc gcgcgatgtc cgggatcaga
     1621 tcgtcatact gcgatccgat gtaccgcgga cccagctgcg cttgaaaggc ggcgtcttcc
     1681 ggtttgctgc gattgtgcat caacacgatc ggcacgccgc gctgtgccgc gagcgcgccc
     1741 atgtccggat cgcgccgcaa gccccacaca tcgttgatca gcgccgcacc cgcgtctaat
     1801 gcgcggcggg ctacttcagc tttgtaagta tcgatcgaaa ttgggccaag gccgtgctgg
     1861 gtcaacgctt cgatcaccgg tatcactcga tcgatctctt catcgatcga gatcgtgcgc
     1921 gaacccggtc gagtggactc gccaccgaca tcgatcaagt gcgcacccga ttggatcatc
     1981 tcgcgcgccc gatcgatcga cgcggcgatc caatctttct cagtcaacat gccgtcgcca
     2041 ctgaatgaat cgggcgtgac attaaggatg cccatcacat aggtctgtcg atcccagtgc
     2101 agccagcgcg atccgacgcg cagcggtcgg cgcgcaaata tttttacgcc ggaagcatcg
     2161 actcgatcgc gcaactctgc gatcgtttga tgcagtaccg gatgaatcag ctgatgcgcg
     2221 atctccgcga agggcaccaa gacaaacgcg cgctcagcca gtgcggcgtg agggatatga
//
LOCUS       DASRTJ010000328         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_321841,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000328 DASRTJ010000000
VERSION     DASRTJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321841"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFF70_06695"
     CDS             complement(<1..275)
                     /locus_tag="VFF70_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44420.1"
                     /translation="MNKAAMIAGLGFIGFFVVLAGFIGSRLNEQTVTMLAGLTCGVGV
                     AMPIGAAVGWYFQGRRSNDRVTVPAQQPMMIVTQPQPSSTPQINPGYA"
     gene            complement(337..1464)
                     /locus_tag="VFF70_06700"
     CDS             complement(337..1464)
                     /locus_tag="VFF70_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZY44421.1"
                     /translation="MQRQELEFQADRIEMVLRDYKAPARVTGGNVTPRWVQFLLQTAP
                     GIKVNRVESLSREIAMALGATSARVTAQGGAVRVDVPRSDPQPVKLLPLCSRLPQIPF
                     GTALLGLADDGAPLLLRLPSPDVAHVLIAGTTGSGKTALVQSMIASLALTHRRSQMQF
                     VLIDPKGRAFEGLNTLPHLLRPIVSQADQIVQVLNDMIELMETRDRSRVTDPRIVIVL
                     DELSDLVQTGGSQVIESLGRLVQRGREAGIHIIGATQKPSSAILGPLVKANFPVRLVG
                     RVVSSDDARVAAGIGGTGAERLTGHGDFVAVAGPGVTRFQAAYISPNDMASVARRLAS
                     GERGLEITRSNETSSRPSFRSTINADEPPLMQAMRAIRRVK"
     gene            complement(1497..2105)
                     /gene="lexA"
                     /locus_tag="VFF70_06705"
     CDS             complement(1497..2105)
                     /gene="lexA"
                     /locus_tag="VFF70_06705"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZY44422.1"
                     /translation="MKSISLKQQAMLAFIEEFMADRGYPPTYEEIRVGMKLSTKSLVD
                     YHLTALEEAGKIQRDPLTPRGLRLPNANTHSVPFVGVIGASLPATTFTDVAAEALILT
                     RDIVPEQDDLFALKVRGTSMIDAMVNDGDVVVMKRQSIVKDGDMVAARLIDRDEMTLK
                     YFYREPGRVRLQPANPMLGPIYTHPANIEIQGRVVAVIRQVH"
     gene            complement(2306..2947)
                     /gene="lexA"
                     /locus_tag="VFF70_06710"
     CDS             complement(2306..2947)
                     /gene="lexA"
                     /locus_tag="VFF70_06710"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZY44423.1"
                     /translation="MEILSTRQQAILDFIRSFSSSSKYPPTIREIGKAVSISSTSVVN
                     YNLNILERKGLIERDRDVSRGIKLVGEAAAESLNTLRIPVLGRIAAGQPIPVPGSNFA
                     ILGDEMIEITRELLPTESKDIYALQVRGDSMVDAMIGDGDIVVMQHRQRADNGDLVAV
                     WIKDKEATTLKRFYLEKGRVRLQPANPTMSPIYEDPKNVEVQGKVVLVIRRPN"
BASE COUNT          734 a          882 c          827 g          753 t
ORIGIN      
        1 gcatatcccg gattgatttg tggtgtcgaa gatggttgtg gctgcgtgac gatcatcatc
       61 ggttgttggg caggtacggt tactcgatcg tttgatcgac gtccctggaa ataccagccg
      121 accgctgcac cgattggcat cgccacgccg acgccgcagg tcaacccggc cagcatcgtc
      181 accgtttgtt cgttcaatcg gctgccgata aagcccgcca gcacgacgaa gaacccgatg
      241 aaacctagtc ccgcaatcat cgccgctttg ttcatgagaa tagctcacaa ggcttaatgc
      301 ataatgcaca attgcatttt gcaacttgca tcttgatcac ttcactcttc gaatcgcccg
      361 cattgcctgc atcaacggcg gctcatccgc attgatcgtc gatcgaaacg aggggcggct
      421 tgatgtttca ttcgatcgtg tgatctcaag gccgcgttcg ccggatgcca atcggcgcgc
      481 gacgctcgcc atgtcatttg gcgaaatata ggcggcttgg aaacgagtga cgcccggccc
      541 ggccactgcg acaaaatcgc catgtcccgt caagcgctct gcgcccgtcc caccgatgcc
      601 tgccgccacg cgcgcatcat cgctgctcac cactcgaccg actaagcgta cggggaaatt
      661 tgctttgacc aatggtccta aaatagcgct cgatggtttc tgtgttgcgc caatgatatg
      721 aatgccggcc tcgcgtccgc gttgaaccaa gcggcccaaa ctttcgatca cttgactgcc
      781 acccgtctgc actaagtcgg acaattcgtc caacacgatc acgatccttg gatcagtcac
      841 ccgcgatcga tcgcgggttt ccatcagctc gatcatgtca ttcaaaactt gtacaatttg
      901 atcggcttga gaaacgatcg ggcgcagcag atgcggcaga gtattgaggc cttcaaacgc
      961 gcgtcccttc ggatcgatca gcacgaactg catttgcgat cgacggtggg tcaaagcaag
     1021 tgaggcgatc atcgattgca ccaacgcggt cttgccagaa ccggttgtgc ctgcgatcag
     1081 cacatgcgcc acatcaggcg aaggcaggcg cagcaataac ggcgcaccat cgtccgccaa
     1141 accgagcagt gcggtgccaa agggaatttg cggcaaacgc gagcacagcg gcagcagctt
     1201 tacgggttgt ggatcggatc gagggacatc cacgcgcacc gcgccgcctt gagcagtcac
     1261 gcgtgcgctg gtcgcgccaa gtgccatcgc aatttcacgc gacagcgact caacgcgatt
     1321 gactttgatg ccgggggcgg tctgcagcag gaactggacc caacgcggcg tgacgttgcc
     1381 gccggttaca cgtgccggtg ctttataatc gcgtaacacc atctcgatcc gatccgcttg
     1441 gaattccagt tcctgtcgct gcataggtca cctctaatat gttgcccaat aaagattcaa
     1501 tggacttgtc gaatcacggc gacaacgcgt ccttgaattt caatattcgc ggggtgcgta
     1561 tagatcggtc ccaacatcgg attggcgggc tgcaatcgca cgcgacccgg ctcacgataa
     1621 aaatatttga gcgtcatctc gtcccgatcg atcaagcggg cggccaccat gtcgccgtcc
     1681 ttcacgatcg actgacgttt catcacgacc acatcgccat cgttgaccat cgcatcgatc
     1741 attgacgtgc cgcgcacctt cagtgcgaac aagtcatctt gttccggcac aatatcgcgc
     1801 gttaagatca gcgcttcggc agccacgtca gtgaaagtgg tggccggcag actcgcgcca
     1861 atgacgccga cgaatggcac actatgcgtg ttcgcattcg gcaagcgcaa gccgcgcggt
     1921 gttaatggat cacgctgaat cttgccggct tcttccaatg cagtcaggtg ataatcgacc
     1981 aatgatttgg tcgataactt catgccgaca cgaatctctt cgtaagtcgg cgggtagcca
     2041 cgatcggcca taaactcttc gatgaaggcc aacatggctt gctgctttag gctaatgctt
     2101 ttcatgctta ggttcccttt ccgagcgttt tgaggtttag gacatttttt ggacttagaa
     2161 cgcgcgttct attattgggt attatataga acttgtgttc tattgtcaat aggcagatcc
     2221 ttaaaaataa acacagcgcc cgatcggcct aggagaccga tcgggcgctg gaaaaaaccc
     2281 gaaaaattga tcgactgaaa tgagcttagt tgggacgtcg aattactaga accaccttgc
     2341 cttgaacttc gacgttcttg gggtcttcat agatggggct catcgttgga ttggcgggtt
     2401 gcaatcgaac acggcccttc tcaagataaa aacgcttgag cgtagtggcc tctttgtctt
     2461 tgatccaaac agcgacgagg tcgccattgt cggcgcgctg tcgatgctgc atcacgacga
     2521 tatcgccatc gccgatcatc gcgtcgacca tcgagtcgcc acgcacttgc aaggcgtaaa
     2581 tatctttgga ctcggtgggc agcagctcgc gcgtgatctc gatcatttca tcgcctaaga
     2641 tcgcaaaatt cgagccgggc accggaatcg gctggccggc agcaatgcgg cccaagaccg
     2701 gaatgcgtag tgtgttgagc gattcggccg ccgcttcgcc gacgagtttg atgccgcgcg
     2761 acacatctcg atcgcgctcg atcagccctt tgcgctccaa gatattgagg ttgtaattca
     2821 cgacggaggt cgatgagatg ctgaccgctt tgccaatctc gcggattgta ggcggatatt
     2881 tgctcgaact tgaaaacgat cgaataaaat ctaagatcgc ttgctgtcgg gttgataaaa
     2941 tctccatgtt ctgctcctcg atctcgtttt ggataaccca aagttatccc aatgatttcc
     3001 caaactctag gcggattata cacgaacagg cgttcggtgt caatacaaag tcgaacatat
     3061 tttcaacgta aatcaacttt attatgttat atgtcaattt gtttcgtcaa ttaaaggata
     3121 gtcataaacc ctctgatttc ccaagcagga actaggtgag aagggatatg atttgcccga
     3181 tcgagcaggt ttcatc
//
LOCUS       DASRTJ010000329         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_273285,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000329 DASRTJ010000000
VERSION     DASRTJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273285"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFF70_06715"
     CDS             <1..341
                     /locus_tag="VFF70_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44424.1"
                     /translation="LSVNPIARAIDPGGSTNYRLIFQPSGGFAGSITITTSSPSPSVT
                     LNLSSLVITPSINVTLTLTDTHPIGLLQPGVYYSVPITATSGSITRTASIGLVVGGWK
                     VYLPINLKNH"
     gene            complement(418..>1712)
                     /locus_tag="VFF70_06720"
     CDS             complement(418..>1712)
                     /locus_tag="VFF70_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44425.1"
                     /translation="AGLLDEYGVDVGRMQLYPPTASYDYMGYAGSEDNSWTSVYTYRA
                     MAGAIRSVSEAGSKTHLAMPANPQLAATAEFLVASGSIGHSTLDIQQGFYRTTLLART
                     TDHLPFGPYSIELQTANGHVLLSREFGPLELSNEEPTDEGLFQMMVPSIKGTTTIVFK
                     YNGVEIGRRAASAHAPSVQVTAPNGGETWSAAGNQTIRWTASDADKDTLTFSVLYSPD
                     KGNTWHTLIANTHELQLTLDSASVHGGDAAFIRVMATDGFNTVEDLSDAAFTVAAKGP
                     DVYIGSPVDGQHILEGFPVVLQAYGTDLEDGPLPSTAFSWSSDKDGPLGNGDQLLADH
                     LSVGEHTITLTGKDSAGNAGTYSVKITIDAKPPLAPNSPIDEQSTPAPAATAQPSAPT
                     ATVAPTTGGNTSVAVIGLMLVALGLVLLVVIVALLRRK"
BASE COUNT          350 a          505 c          468 g          389 t
ORIGIN      
        1 acctgagtgt caatccgatc gcgcgcgcga tcgatccggg cggctcaacc aactaccgat
       61 tgatcttcca gcccagcggc ggcttcgccg gatcgatcac tatcacgacc tccagtccgt
      121 cgcccagtgt cacactgaat ctttcatcgc tcgtgatcac gccatcgatc aatgtcacac
      181 tcactttaac cgacacccat ccgatcggtt tgctgcaacc cggcgtgtat tactctgtgc
      241 cgatcactgc aaccagcggc agcatcacac gtacggcctc gatcggtttg gtggtcggcg
      301 gctggaaagt gtatctgcca attaatttga aaaaccattg actcgaacat tggctaaaac
      361 gaaacgcggc gatcgcttca tcaacgatcg ccgcgttcga tttgatttaa gcgtctgcta
      421 ttttctgcgc agcaaggcca cgatgaccac caacaacacc agacccagcg cgaccagcat
      481 caagccaatc accgcaactg aagtgttgcc gcccgtcgtc ggtgcaacag tcgcagtcgg
      541 tgcactaggc tgtgcggtcg ctgcgggtgc aggagttgac tgctcatcga tcgggctgtt
      601 cggcgcgagc ggcggtttgg catcgatcgt gatcttcacc gagtaagttc cagcattgcc
      661 agctgaatct ttccccgtca aagtaatcgt gtgttcgccc acggataaat gatcggccag
      721 taattgatcg ccgttgccta atggcccatc tttatcagat gaccagctga atgccgtgct
      781 gggcagcggt ccatcttcga ggtcagtgcc atacgcttgc agcacgaccg ggaatccttc
      841 caggatgtgc tgcccatcca ccggcgagcc gatgtaaacg tccgggcctt tggccgccac
      901 cgtgaaagcc gcgtccgata aatcttcgac ggtgttgaaa ccatcggtgg ccattacccg
      961 gatgaaagct gcatcaccgc catgcacact ggcgctatca agggtgagtt gtagctcgtg
     1021 agtattcgcg atcaaggtgt gccacgtgtt gcctttatcc gggctgtata acacgctgaa
     1081 ggtgagcgtg tctttatccg cgtcgctcgc ggtccacctg atagtttgat tgccggccgc
     1141 gctccatgtt tctccgccgt tcggcgcggt gacttgaacg ctcggcgcat gagcgctggc
     1201 cgcgcgccgt ccgatctcga caccgttgta tttgaagacg atcgtcgtag tgcctttgat
     1261 ggacggcacc atcatctgaa ataagccttc gtcggttggc tcctcgttgc tgagctcaag
     1321 cggaccgaac tcgcggctca ataatacgtg gccgttagcc gtctgcagtt cgatcgagta
     1381 cgggccaaac ggcaggtgat cggttgtgcg cgccagtaat gtcgtgcgat agaagccctg
     1441 ctgaatatcg agcgtgctat gtccgatcga tccactggcc actaagaatt cagccgtggc
     1501 ggccagctgc ggattggccg gcatagcgag gtgggttttc gatcccgctt cagacactga
     1561 tcgaattgcg ccggccatgg cgcgataggt atagaccgat gtccaagagt tgtcttcact
     1621 gcctgcgtaa cccatgtaat catatgaagc ggtcggcgga tataactgca tacggccgac
     1681 atccacgccg tattcatcca gcaggccggc gc
//
LOCUS       DASRTJ010000330         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_246380,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000330 DASRTJ010000000
VERSION     DASRTJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246380"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFF70_06725"
     CDS             complement(<1..281)
                     /locus_tag="VFF70_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY44426.1"
                     /translation="MSSIVTSQGVVHYETYGRGRPVILLHGWINSWGVWQDVMIDLAE
                     NAHYRVYALDFWGFGESAKTNTPPFKIRSYVQMVDQFMEILGIERAPVMG"
     gene            complement(319..858)
                     /locus_tag="VFF70_06730"
     CDS             complement(319..858)
                     /locus_tag="VFF70_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HZY44427.1"
                     /translation="MQTVKMVVTGPFAAGKTEFIRTISEIDVVSTERKISTDAERIKD
                     ATTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVIVDSSRPETFRE
                     ARSILDTFRGYAATPFVIAANKQDTEDAWTPDDLRIALKIDPNTKVMPCTATNRDSVK
                     GVLLELLYSILESMDSESQ"
     gene            complement(908..1723)
                     /locus_tag="VFF70_06735"
     CDS             complement(908..1723)
                     /locus_tag="VFF70_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZY44428.1"
                     /translation="MALKGNLHDFSITQLLNLIHLARKTGTLAIDGKSSATRVAFREG
                     KLIHALSTGAEGRLVILLIRAGKITADQGKQIPENKTDRELGAMLVQAGLLTKQDMDS
                     LVRNHVREVVEKLFALSEGYFRFEPNILPDDRLVADPPISLDNIIMEGTRRFKESERL
                     QDELPNLDITMKFTDRPDTRLRTVNLSVEEWRVVSYINPKNTIRQIAQANGMSEFQIR
                     KIVYGMVQAGLVELLQPAGGLALQAGHVPVGTSFKPEEKRSIVNRLIARIKQL"
     gene            1941..3170
                     /locus_tag="VFF70_06740"
     CDS             1941..3170
                     /locus_tag="VFF70_06740"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZY44429.1"
                     /translation="MFDRFFIFTKSNMPMNFFVSRVIRSVWLMIPIVLLAACGSSNPA
                     TSTPRPTLTQIPIIKPTTFPTVTPLPTAAPTVDPHVVATFAPDVNPLTGEKVSDPSVL
                     DRRPLEIVIANSNEIGVRPQSGASYADWVFEHETEAPGITRWGAIFYSRAPNGVGSDR
                     SCRIIDDELPAIFKSLLACSGMSGGTREFYIKPSDFNQEQRFFSPDFGDGAPMFYRTD
                     TAAPPHNLFVNPAEVWKAADQRGSNSKPDLKGFAFAANPIAPGAPATDVALAYHSSTV
                     EWKYNANATTCSSLDGCYLRWNDGALHTDALNGQQLSAANVIVIYVNYTEDHRYLEEE
                     YGALKLYGWQIQLWGDSAGNVQIFRDGQEFDGQWSRPTRPDTLSFNDAKGQPIPLKPR
                     NMWVELVKWDANVTVKP"
     gene            complement(3304..>4000)
                     /gene="serS"
                     /locus_tag="VFF70_06745"
     CDS             complement(3304..>4000)
                     /gene="serS"
                     /locus_tag="VFF70_06745"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZY44430.1"
                     /translation="VYPPYLVKREALLGTGQLPKFSELQYFDQADDLWMIPTAEVPVT
                     NMYAGEILDGSRLPINHVAYTACFRREPMSAGRDTRGIKRGHQFDKVEMVKFTLPESS
                     IAEFSKLLDNAEEICRLLKIPHRIIQMVTGDLSFTAAIKMDVEMWAPGCDEWLEVSSV
                     SNFKDFQARRANIKFKRDESSKAEYVHTLNGSGLALPRVMIAVMENYQQPDGSIKIPE
                     VLIPYMGGVEVIK"
BASE COUNT          970 a         1142 c          925 g          963 t
ORIGIN      
        1 cccatgaccg gcgctcgttc aatgccaagg atctccatga actgatcgac catctgcaca
       61 taggatcgaa ttttgaatgg cggagtattg gtttttgccg actcaccgaa tccccaaaag
      121 tccagtgcgt aaacccggta atgggcattc tccgcgagat cgatcatgac atcttgccat
      181 acgccccacg aattaatcca gccgtgcagc aggattactg gacgaccacg gccgtacgtt
      241 tcataatgga cgaccccttg cgaagtaact atcgaactca taattgatca taacaggtag
      301 atgactaagc ccgtcaaatt actgcgactc cgaatccata gattccaaaa tcgaatacaa
      361 tagctcgagc aacacacctt taacgctatc gcgattggtc gccgtgcacg gcatcacttt
      421 ggtgttggga tcgatcttca acgcaatacg aagatcatcc ggcgtccacg cgtcttcagt
      481 gtcctgcttg ttcgccgcga tcacaaaagg tgtagcggca taaccgcgga aagtatccaa
      541 aatactgcga gcttcacgga aagtttccgg tcgcgaacta tccacaatca cgatgaaacc
      601 cagcatgcct tcagataaaa tctcccacat gaagtcgaac cgcttctgac ccggcgtgcc
      661 gaacagatac aagaccaagt cctcatcgac cgtaatgcgt ccgaagtcca tcgccacggt
      721 cgtggcatcc ttgatccgct cggcatccgt ggagatttta cgctccgtcg agaccacatc
      781 gatctcgctg atcgtccgaa tgaattcggt tttacctgca gcaaacggtc cggtcactac
      841 catctttact gtttgcatac gcgcccacta tcccttcact tgggaaataa aactaactac
      901 gccagtctta caactgcttg atgcgagcaa ttaatcgatt aactatcgag cgtttttctt
      961 ccggcttaaa tgaagtgccc accggcacgt gtccagcttg cagcgccaaa ccacccgccg
     1021 gctgcaacaa ctcaaccaac cccgcctgca ccataccgta aacgatcttg cgaatctgaa
     1081 attcgctcat gccattggcc tgggcaattt gtcgaatcgt attcttcgga ttgatatagg
     1141 acacaacccg ccactcttcc acgcttaaat tcacggtgcg cagccgggta tcaggtctat
     1201 ccgtaaactt cattgtaata tcgaggttgg gcaattcgtc ttgaagccgc tccgattctt
     1261 tgaaacggcg agtaccttcc ataataatgt tgtccagact gatgggcggg tcagccacca
     1321 accgatcatc cggcaagatg ttcggctcga agcggaaata gccttcgctc aacgcgaaca
     1381 acttttcgac cacttcgcgt acgtggttac ggaccaagct atccatgtcc tgcttcgtca
     1441 acaacccagc ctgcaccaac atcgcgccca attcgcgatc ggttttgttt tctggaatct
     1501 gtttgccttg atcagcagta attttgccag cacggatcag caaaatcacc agccgccctt
     1561 cggccccggt cgacaatgca tgaattaact tgccctcccg gaatgccacg cgcgtcgcgc
     1621 tactcttccc atcaatcgcc aatgtgcctg ttttgcgcgc tagatggatc aggttgagca
     1681 gctgcgtgat gctgaaatcg tgtaggtttc cttttagtgc cataggcgct caccttgacg
     1741 agttttttga agtccgcggg actgatcagc cgatatagtt cgaacagacg agcaacgatt
     1801 cacacgatcg cgctcgtttt acgcacctca tatattgcgc gacgattata cctgcgaagg
     1861 aaatgcaagt caacttaatt cgctccctcg ccccatcaga ttgcggcaaa atcttgcgat
     1921 ccttatgcta taattgctca atgttcgatc gattcttcat cttcacgaag agtaacatgc
     1981 cgatgaattt tttcgtttcg cgggtaatcc gatcggtttg gctgatgatc ccgatcgttt
     2041 tgctggctgc ctgtggatcg tccaatcctg caacctcaac accgaggcca acgctcacgc
     2101 aaatcccgat catcaaaccc acaacatttc caacggtcac gcccttaccc actgccgcgc
     2161 cgactgtcga tccacacgtg gtcgccacct tcgcgccgga tgttaatccg ttgacaggcg
     2221 aaaaggtttc cgatcctagc gtattggatc gacgcccgct agagattgtc atcgcgaact
     2281 ccaatgagat cggtgtccgg ccgcaatcgg gcgcgagcta tgcggattgg gtgttcgagc
     2341 atgaaaccga agcacccggc atcactcgtt ggggcgcaat tttttacagc cgcgcgccta
     2401 acggcgtcgg ctcggatcgc agctgtcgaa ttatcgatga tgaactgccc gcgatcttca
     2461 aatcgctcct ggcctgctcc ggcatgtccg gcggtacacg cgaattctat atcaagccca
     2521 gcgactttaa tcaagaacaa cgttttttct cgccggactt tggtgatggc gcgccgatgt
     2581 tctatcgcac tgatacggct gcaccgccgc acaacctatt tgtcaatcct gccgaagttt
     2641 ggaaagccgc cgatcaacgc ggctcaaaca gcaagcccga tctgaaaggt tttgcgtttg
     2701 cagccaaccc gatcgcgccc ggcgctcccg cgaccgatgt ggcgctagct tatcactcgt
     2761 ccacggttga atggaagtat aacgccaacg ccaccacttg tagttcatta gatggctgct
     2821 atttgcgctg gaacgatggc gctcttcata ccgacgcact taacggccag cagctcagcg
     2881 ccgcaaatgt gatcgtcatc tacgtcaact acacggaaga tcaccgttac cttgaagaag
     2941 aatatggcgc tctcaagtta tacggttggc agattcagct gtggggcgat tccgctggca
     3001 atgttcaaat cttccgcgac ggccaagagt ttgatggaca atggagccgc cccacccgac
     3061 cagatacgct cagttttaat gatgctaaag gtcagccgat tccactcaaa ccccgcaaca
     3121 tgtgggtgga gttggtaaaa tgggatgcga acgtaacggt caaaccgtaa atcagcgtat
     3181 cacatctgcc cttcaatcag gattgcaagg tggttacaag ttcataaaaa acctgcgagg
     3241 tagtgaaaac ctcgcaggtt tctgattgtt ccgcgttcga tttctagcga gcagccgaat
     3301 cctctacttg attacctcaa caccgcccat gtaaggtatc aagacctctg gaattttgat
     3361 cgatccatcg ggctgctggt agttctccat cacggcgatc atcactcgcg gcaaagccaa
     3421 acctgatcca ttcagcgtgt gaacgtactc cgccttggaa ctttcatcgc gcttgaattt
     3481 gatgttagcc cgtcgagctt gaaaatcttt aaagttgcta accgatgaaa cttcaagcca
     3541 ttcatcgcaa ccgggcgccc acatctcgac atccattttg atagccgccg taaatgatag
     3601 atcgccggta accatttgaa taatgcgatg cggaatcttc agcaagcggc aaatttcttc
     3661 ggcgttatcg agcagcttgc taaattcggc gatgctcgat tcaggcaagg tgaacttcac
     3721 catctccact ttatcaaatt ggtggccgcg cttgatgccg cgcgtatcgc gtcccgccga
     3781 cattggctcg cgccggaaac aagccgtata ggccacgtga ttgatcggca aacgagatcc
     3841 atctaggatt tcaccggcgt acatattggt cactggaact tcagcggtcg ggatcatcca
     3901 cagatcgtca gcttgatcaa agtattgcaa ctcggaaaat ttgggtagct gtccagtgcc
     3961 gagtaacgcc tcgcgcttga cgagatacgg cggataaact
//
LOCUS       DASRTJ010000331         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_322099,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000331 DASRTJ010000000
VERSION     DASRTJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322099"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFF70_06750"
     CDS             complement(<1..782)
                     /locus_tag="VFF70_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HZY44431.1"
                     /translation="MSDSGALIRTPDQQVRVFVSSTLQELADERAAVRDAILQLHLAP
                     VMFELGARPHPPRELYRAYLDQSHIFIGIYWQKYGWVAPGETISGLEDEYRLSGDKPK
                     LIYIKSPAHDREPQLKELLDRIRSDDTVSYKPFAAATELRELIENDLMLLLTERFDLH
                     ADRSRPTGTVTFLFTDIEGSTDLAQQYPEALPALLARHHAILQQAIEAHHGFVFQIVG
                     DSFHVAFHTAPDAFNAALDAQRMLHQEAWSPASIKVRMGINTG"
     gene            complement(858..1493)
                     /locus_tag="VFF70_06755"
     CDS             complement(858..1493)
                     /locus_tag="VFF70_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44432.1"
                     /translation="MNLTTTTGIALILSTLIYHGGLAYLFARAQFEEWLNLPQRSYRE
                     KLLIIARHPEEYRWGFRIILIGWIVAALGYVMLTVLLRDAGDPIISTLASVLFLMGIV
                     SAIAFWALSLTANVLAAEHTARTTIVPEYYEVLEMSAESLLGVYQLLALLATAGFGWA
                     LLQTGILPSWVGWVTIGWGLLWAGVHLRSDETIPLLPMVMQVVIGISVLLK"
     gene            complement(1512..1964)
                     /locus_tag="VFF70_06760"
     CDS             complement(1512..1964)
                     /locus_tag="VFF70_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY44433.1"
                     /translation="MNSSLRHIALFVPDLRAAERYYQSVFDMELIGREAELSDGLWYT
                     LPFDKGWDEAEAAGIELGMLALRKDEFGLALFRGDAPAGQVNVIGLRMPVDEIARVRA
                     RLPEDTQVSEDAPDYLEFRDPYQITWQILFPGSKFSTAGVFANRWLKL"
     gene            complement(2269..2361)
                     /locus_tag="VFF70_06765"
                     /pseudo
     CDS             complement(2269..2361)
                     /locus_tag="VFF70_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          592 a          675 c          607 g          537 t
ORIGIN      
        1 cccgtgttga ttcccatccg cactttgatc gaggcgggtg accacgcctc ttgatgtaac
       61 atgcgttggg cgtccagtgc ggcgttaaat gcatccggag cagtgtggaa ggccacatgg
      121 aatgaatcgc cgacgatttg aaatacaaaa ccatgatgcg cttcgatcgc ttgctgcaag
      181 atcgcgtgat gccgcgccaa cagagccggc agcgcctcag gatattgctg cgccagatcc
      241 gtgctgcctt caatgtcggt gaacaggaag gtgacggtgc cggtgggcct cgaacgatcg
      301 gcgtgcaaat cgaagcgctc ggttagcagc agcatcagat cgttttcgat caactcgcgc
      361 aattcagtcg ctgcagcgaa cggcttgtaa gaaacggtat catcactgcg gattcgatcg
      421 aggagttctt tcaactgggg ttcacgatcg tgcgcgggag atttgatgta gatcagtttc
      481 ggcttgtcac cggacaaacg atactcgtct tccaggccgg agatcgtttc accgggcgcg
      541 acccagccgt acttctgcca atagatgcca atgaagatat gactttgatc gagataagcg
      601 cgatacaatt cgcgtggggg atgcggccgc gcgccgagtt cgaacatgac cggcgccaga
      661 tgcagctgca ggatggcatc gcgcacagcg gcccgctcgt cggccagttc ttgcagcgtc
      721 gaactgacga agacacgcac ctgttgatca ggtgtgcgga taagggcacc cgaatcgctc
      781 atgtttcacc cccggccaaa tgacaggcga ttgtattcac tctttgctct ccgaccgcgt
      841 cctcgcgggc agttcattca tttgagtagc acagatatac caatcacaac ttgcatcacc
      901 atgggcagta atggaattgt ttcgtcactt cttaaatgta ctcctgccca gagcaggccc
      961 catccaatgg taacccatcc aacccaactc ggcagaatac cggtctggag cagagcccag
     1021 ccaaatcctg cggtagccag taatgcgagc aattgataga cccccaataa agattctgcc
     1081 gacatttcta ggacttcgta gtactccggc acaattgtag tacgggccgt gtgctctgcg
     1141 gccaatacgt tagccgtcag agacaacgcc caaaaagcga tggccgagac aatccccatt
     1201 agaaacaata ccgaggccag tgtggaaatt atcggatcgc ctgcgtcacg cagaaggacg
     1261 gtcagcatga catatcccaa agccgccaca atccatccaa tgagtatgat gcgaaacccc
     1321 catcgatatt cttctggatg cctggcaatg attaagagct tctccctata agaccgctgg
     1381 ggaaggttta gccattcctc gaattgcgcg cgggcaaaaa ggtaggccag gccaccatgg
     1441 tatatgagtg tactcagaat gagagcaatg cctgtcgttg tggttaagtt catcgagtaa
     1501 ttcctcggcg gtcaaagctt gagccaacgg tttgcgaaaa ccccggctgt gctgaattta
     1561 cttccaggaa acaaaatctg ccaggtgatc tggtaagggt cgcggaattc aaggtaatca
     1621 ggcgcatctt cacttacttg cgtgtcctcc ggcaatcgag ctcgaaccct ggctatctca
     1681 tccacaggca tcctgaggcc aatcacattc acctgccccg caggcgcatc gcctctgaac
     1741 aaggccaaac caaactcatc ctttcgaaga gcgagcatac ctagctcaat gcctgccgct
     1801 tctgcctcgt cccaaccttt gtcgaaggga agtgtatacc acaggccatc cgaaagctca
     1861 gcctcacgtc cgatcagctc catgtcgaat accgattggt aatagcgttc agccgcccgc
     1921 agatcgggga caaacaaggc gatatgtctc aaagaactat tcatctttgc ctctgctaga
     1981 acaaatgcgg gaaatcggct ccaatctcag caaagcatcg gttgccgccc aacaagtgct
     2041 taaagttaaa aattccgtac catatcctct tcagggatgt tgggccgaat tattccgctg
     2101 aatggattat aggcggatta gtcttctgcg ctagtataaa ccaatctcca tctgcgtcaa
     2161 tatagcagca cgacttcaat ccgcttatcg agattgaatt gcgccgcaga acaaaataac
     2221 aacgcccgat caaaagtgat cgggcgttct tgtgctaaat tatccgcgct acttaaagcg
     2281 ttgtatcttg cccgtggccg tcttgggcag ctcgggcaca aactcgatcc agcgcgggcg
     2341 tttatactcc gccatttttt cgctccaagc ccgcgaccgc gctggcgttt ctcaacggcc
     2401 tactcgaccg c
//
LOCUS       DASRTJ010000332         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_57521,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000332 DASRTJ010000000
VERSION     DASRTJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57521"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFF70_06770"
     CDS             <1..246
                     /locus_tag="VFF70_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44434.1"
                     /translation="DLNRGLSLSLPYFDDQNLKMEAHNFEVIPAGRIMFVPAFMVRAA
                     REEQAKVAQDTRLSASTRKYLLAELKQLEQAFESPAK"
     gene            334..>2135
                     /locus_tag="VFF70_06775"
     CDS             334..>2135
                     /locus_tag="VFF70_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44435.1"
                     /translation="MLFSVVLLLVVVFIAWKISPREKKESAHVHGAGQIGLLAAVALF
                     GVDYFTSYFYATGEMMSALHPYNLQNYAYIAVAVIGIANFVFGALYMYSLGVFNEGGG
                     SYTASMRYLLPTLSLIVAVTLIQDYVLTIVVSALSGGDQLLSVVGAYGTDWFWHFAIG
                     AALAFVTWYLTIRGRGESATVVFTLLSIFIMLSIALFVGLVLAYNRGVAALPVEPVTD
                     IPSVGQALLHMLTASMKGMVALSGLEAMSNGIQFVIDEDAGIVKWGKQHLPKLAGLWK
                     FYSGKSGIGRFVQTSFLFYGGLTTLFLTFFAIHFNAFDGTQGRSLVGNLAFIGFDQLP
                     GGNILFFAYQFLAVALLAAASMTAFQDAQATEWRDVAIGEIPEVIVYRDPRGTFTRSV
                     TITFGLAVLIMFVVRGETTKAVPFYGVGVFMPIMVMGLAIRQHVLKHFTGRKRTLGAF
                     GAGLAAALGGIVFIGQIVGKWEEGGWVVLISFSLLALLAHGLLLSPTGYRDAKQIHRI
                     VRDKARVQGSMASIVEWQSLRMQEYRYGVLSLISRFFGLFGLHKPVRYARPVEAGTYD
                     TALHSDHPDAPSFLEQYLDKPEPHLGGAPNVTAKP"
BASE COUNT          404 a          529 c          625 g          577 t
ORIGIN      
        1 gatctgaatc gtggtctcag tctgagtttg ccgtacttcg acgatcaaaa cttgaagatg
       61 gaagcacaca attttgaagt gattccggcg gggcgtatca tgtttgtacc cgcgttcatg
      121 gtgcgggcag cgcgcgaaga acaagccaag gtcgcccagg atacgcgctt aagcgcatca
      181 actcgaaaat atctgttagc tgagctcaag cagttggagc aggcttttga atcgcccgct
      241 aaataattta gtgcaataaa gaaattggaa gtcggagcgc tgaatatgct ctgacttcca
      301 atttctattg tctagtccgg ataggtgaga ttcatgttgt tttcagttgt tttactgtta
      361 gtcgtggtct tcattgcgtg gaaaatttcg ccgcgcgaga aaaaagagag cgcgcacgtg
      421 cacggcgcgg gccagatcgg tttactggct gcagtggccc tgttcggcgt ggactatttc
      481 acctcgtatt tttatgcgac cggtgaaatg atgagcgccc tgcatcctta caatttgcag
      541 aactatgcct atatcgccgt cgcagtgatc ggtattgcca actttgtgtt tggcgcattg
      601 tatatgtatt cgctgggcgt attcaatgaa ggcggcggat catatactgc gtcgatgcgt
      661 tatctgctgc cgacgctgag cttgatcgtg gcggtgacgt tgattcagga ctacgtgctg
      721 acgatcgtgg tttcggcttt atcaggcggc gatcagttgt tatcggtcgt gggggcatac
      781 ggtaccgatt ggttctggca tttcgccatc ggcgcagcgc tggcctttgt cacgtggtat
      841 ctcaccattc gcggacgcgg tgagtcggcg accgtcgtct tcacactgct cagcatcttt
      901 atcatgttgt cgatagcgct cttcgtgggc ttggtgctgg cctacaatcg cggcgtcgcc
      961 gcattgcccg tcgagccggt aaccgatata cccagcgtcg gccaagcgtt gctgcatatg
     1021 ttgacggctt cgatgaaagg catggtcgcc ttgagcggtc ttgaagcgat gtcgaacggt
     1081 attcaatttg tcattgacga agatgccggc atcgtaaaat ggggcaagca gcatttgccg
     1141 aaactggctg gtctctggaa attctacagc ggcaagtcag gcattggccg ttttgtgcaa
     1201 acttcgtttt tgttttatgg cggcctcacc actttgttcc tgaccttctt tgcgattcat
     1261 ttcaatgcgt tcgatggcac acagggacgc tcgctcgtgg gaaacttggc gttcattggc
     1321 tttgatcagt tacccggcgg gaatattttg tttttcgctt atcaatttct cgccgtcgcg
     1381 ctgctggccg cggcttcgat gacggccttt caggacgcgc aagcgaccga gtggcgcgat
     1441 gtggcgatcg gcgagatccc ggaggtgatc gtatatcgtg atccgcgcgg cacgtttacg
     1501 cgatcggtca ccatcacctt tggcctggcg gtcttgatca tgttcgtcgt gcgcggcgaa
     1561 acgacgaagg ccgtcccgtt ttatggtgtc ggcgtcttca tgccgatcat ggtcatgggt
     1621 ctcgctattc gtcagcatgt gttgaagcac tttacgggac gcaagcgaac gctcggcgcg
     1681 tttggcgcag gcctggccgc cgctttgggg ggtattgtct tcattgggca gattgtgggc
     1741 aagtgggaag agggcggttg ggtcgttttg atctcgttca gtctgttggc cttgttggcg
     1801 cacggcttat tgttgtcgcc caccggctat cgagacgcga aacaaattca ccggattgtg
     1861 cgcgataaag cgcgtgtcca gggttccatg gcttccatcg tcgagtggca atctctgcgc
     1921 atgcaagaat atcgctatgg cgtattgagc ttgatctcgc gtttcttcgg attgttcggc
     1981 ttgcacaaac cggtgcgtta tgcgcggccg gtcgaggctg gcacgtacga tacagccctg
     2041 cacagcgatc accccgatgc gccatccttc ttggaacaat atcttgacaa gcctgagccg
     2101 catctcggcg gagccccgaa tgtaacggcc aagcc
//
LOCUS       DASRTJ010000333         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_348900,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000333 DASRTJ010000000
VERSION     DASRTJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348900"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VFF70_06780"
     CDS             <1..1370
                     /locus_tag="VFF70_06780"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZY44436.1"
                     /translation="RGAIDFWGPWQRVETGYLGVVPLLLILFAFTRKNLRPVLFLATL
                     GLIGLLIAFGKFTPIYSLFHALPILNGLRVPARFILVTDFSIAALAALGLQNLLNAEW
                     GRRDVITRSAGLIVIGIVSLWVAYQSVGNSEHLGSLLSASIFFAAVALNAGFLIVLRM
                     KLRITNFKLVTILIALMAFELIFLGSTIEIDANDPVAGFVHPQVVEFLHSDPNPYRID
                     TSSAVWQPDAALLNGLDDIGGIFNPLGLANYETYRGGMGNRGSALYNFLGVKYVLAPK
                     DHPPGDASFVPVFNTDPWIDVFLNTRALPRAQLIDRATVVSSGEEAWSAIHQANFDPS
                     KEVVIQGGESRSSAGGQASNRSIAFGSISNERIELNVQLDSATYLVLSDVYYPGWIAS
                     IDDRATEIYPANFSFQAIEVSSGSHHIVLQFDPVSWKVGLSISLLTALVLVFWLPVRW
                     KLLHQ"
     gene            1448..1663
                     /locus_tag="VFF70_06785"
     CDS             1448..1663
                     /locus_tag="VFF70_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44437.1"
                     /translation="MTGSDARTNKLKFWMFGIFVLVSAAVTAVLAAVGGGNIGVVIGE
                     MNYWLMIAAAAVLCIVGYLVLVRVGKK"
     gene            complement(1699..2850)
                     /locus_tag="VFF70_06790"
     CDS             complement(1699..2850)
                     /locus_tag="VFF70_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY44438.1"
                     /translation="MDFQLNEEQQLFQRAVRDFVEAEVKPRARHTDEAAEFNWAAVKK
                     MGPIGLLGLNVSEEYGGAGVDAISAAIAIEELGRGCGSTALAIAAHNSLASAPIALFG
                     SEALKKKYLPPLATGSGKLGALALTEPGAGSDLAGGVKTFAEKKGAEWIINGSKMWCT
                     NASIADVIVTLCRTDRSGGSSSMVLILVPTNTPGLIIGPAEKKMGLKGSPTHAVTYDN
                     VRVPIENLLEAEGAGLRQTFQVLDGGRIGIGALSIGLAQAAHEESIKYAKERHTFGKP
                     IAEYQAIQWMIADSATEIDAARFMVYRAAWLKQNGKSFAREAAMAKLFATEMAERVCF
                     NAIQIHGGYGYSAEFPVERMYRDQRLMQIGEGTSEIQRLVIARGVLGLK"
     gene            complement(2963..3481)
                     /locus_tag="VFF70_06795"
     CDS             complement(2963..3481)
                     /locus_tag="VFF70_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HZY44439.1"
                     /translation="MNSLRKLFTAIDVRLTHWMARYGITLLRISLGIVFFWFGVLKFF
                     PGLSPAQELATRTIGILTFGLVPSQTSILILATWECLIGLGLIIGVFMRVTLLLLWLQ
                     MLGTLMPLILFPQEAFIYFPYAPTLEGQYIIKNIVLISASIVIGATVRGGTLVADSPP
                     NKPDSVNSGLKS"
     gene            complement(3691..>4171)
                     /locus_tag="VFF70_06800"
     CDS             complement(3691..>4171)
                     /locus_tag="VFF70_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY44440.1"
                     /translation="IVVTAGYSYPAMGAGLLITPNYGIPAIITLTNTLIGDNALHVGI
                     NAPVSDDCYGKVRAKGYNLIETMAHCQFEGPSIGDVLGIDPKLGPLQLNGGSTLTQSP
                     LPGSPAIDAGRPTGCTDDLGALLTIDQRGWRRPIGSHCDIGAVEYSPFAIDLPLVRR"
BASE COUNT          905 a         1068 c         1146 g         1052 t
ORIGIN      
        1 gccgtggcgc gatcgatttt tgggggccgt ggcagcgggt cgagacgggc tatctgggcg
       61 tcgtgccatt gctgttaatc ctgtttgcct ttacgcgtaa aaatttacgc ccagttttgt
      121 ttttagccac attaggtttg atcggtttgc tgattgcatt tggcaagttc acgccgatct
      181 attcgctctt tcacgctcta ccgatcttga atggcttacg cgtaccggcg cgatttattc
      241 tggtgactga tttctcgatc gcagctttgg cggcgctcgg attgcaaaac ctgctcaatg
      301 cagaatgggg aaggcgggat gtgataacac gcagcgctgg cctgattgtt attggcatag
      361 tcagcctatg ggttgcatac caatccgtcg ggaattcaga acatcttggc agcctgttat
      421 ccgcgtcgat atttttcgcg gctgttgcct tgaacgctgg cttcttaatc gtactgcgta
      481 tgaaattgag aatcacaaat ttcaagttgg taaccatttt gattgcactc atggcatttg
      541 agcttatctt tttgggctcg acgatcgaga tcgacgccaa cgatcctgtt gcgggtttcg
      601 ttcatccaca agtggttgaa ttcctgcact cggatccgaa tccgtatcgc attgacactt
      661 cttcggctgt gtggcagccg gatgccgcgc tgttgaatgg cttggacgac atcggtggaa
      721 tcttcaatcc gttgggcttg gcgaattacg aaacgtatcg cggcggaatg gggaatagag
      781 gttcggcgtt gtataacttt ctcggtgtga agtatgtgct cgcgccgaag gatcatccgc
      841 cgggtgatgc ttcattcgtg ccggtgttca atactgatcc atggatcgat gtctttctca
      901 acacacgggc attgcccaga gcacagttga tcgatcgggc gaccgtcgtg tcgagcggcg
      961 aggaggcttg gagcgcgatt catcaggcca actttgatcc atcgaaagaa gttgtgattc
     1021 aaggtggcga aagtcgatcg agcgcgggag gacaagcgag caaccgatca atcgcgtttg
     1081 gatcgatatc gaacgagcgg atcgaactga atgtccaatt agattcagca acgtacttgg
     1141 tgttgagcga cgtgtattat ccgggctgga tcgcttcgat cgacgatcga gcaactgaaa
     1201 tttatccagc caatttttct tttcaggcca tcgaagtgtc gagcggatcg catcatatcg
     1261 tgttgcaatt tgatccggta agttggaagg tcgggttatc gatcagctta ttgactgcgc
     1321 tggtgctggt gttctggctg ccggtccggt ggaagctatt acatcagtaa cattgtgttg
     1381 cgacgtccgt ttggtgtacg atcttcgtgt cattcaatct tttgtctgga ggctggaaat
     1441 gagtcaaatg actggttcag atgcgcggac gaataaactg aagttttgga tgtttggaat
     1501 ttttgtgttg gtctccgcgg cggtcaccgc agtattggcg gccgtgggtg gcggaaatat
     1561 cggcgtggtc atcggcgaaa tgaattactg gttgatgatt gctgccgccg cagtgttgtg
     1621 tatagttggt tacttggtgc tcgtgcgcgt aggcaagaag taaagcgtga ggccgatgat
     1681 caaatcatcg gcctcttttt atttcagccc caacacaccg cgcgcgatca ccaggcgctg
     1741 tatctcgctg gtcccttccc caatttgcat caaccgttga tcgcgataca tgcgctcgac
     1801 aggaaactct gcgctgtaac catagccgcc atgaatctga atcgcattga agcatacgcg
     1861 ctcggccatc tccgtcgcga ataattttgc catcgccgct tcgcgtgcaa aagattttcc
     1921 gttttgtttc aaccacgccg cgcgatagac cataaagcgt gccgcatcga tctcggtcgc
     1981 agagtcggcg atcatccact ggatggcttg atactctgcg atcggctttc caaacgtgtg
     2041 acgctcttta gcatacttga tcgattcttc gtgcgcggcc tgcgctagtc cgatcgataa
     2101 cgcgccgatg ccgatccgtc cgccgtccaa aacttgaaac gtctgccgca aacccgcgcc
     2161 ttccgcttcc agcaaattct cgatcggcac tctgacgttg tcgtacgtca ccgcatgcgt
     2221 aggcgatcct ttaagaccca ttttcttttc agccggaccg atgatcaagc ccggcgtatt
     2281 tgtcggcacg aggatcaata ccattgaact cgatccgccc gatcgatcag tgcggcacag
     2341 cgtgacgatc acatcggcga tgctggcatt ggtgcaccac atcttcgatc cgttgatgat
     2401 ccactcagcg cccttcttct ctgcaaaagt tttcacgccg cccgctagat cgctgcccgc
     2461 tcccggttca gtaagcgcca acgcgcccag cttgcccgac ccggtggcga ggggcggcag
     2521 atattttttc ttcaatgctt ccgaaccaaa caacgcgatc ggtgcagaag ccaagctgtt
     2581 gtgcgcggcg atcgccagcg cggtcgatcc gcagccgcga ccgagttctt cgatcgcgat
     2641 ggcggcgctg atcgcatcga cgcctgcgcc gccatattct tccgacacgt tgaggccgag
     2701 cagaccgatc gggcccatct tcttgacggc cgcccagtta aattcggcgg cttcatcggt
     2761 gtgccgcgca cgcggtttca cttcggcttc gacaaaatca cgtacggcgc gttgaaagag
     2821 ttgctgttct tcgttgagtt gaaagtccat tcgagcgata atctcctgat aaagtctgaa
     2881 cgaacccaat catatcatag tcgtcaggag atcgacgatc gggctattta tagcgaggct
     2941 ctcagatcga ggttgggttg agtcatgatt taagccctga attgacgctg tccggtttat
     3001 tcggcggaga atccgctact aatgtcccgc ctctcacggt tgcgccaata acgatgctgg
     3061 cgctgatgag cacgatgttt ttgataatgt actggccttc gagtgtgggc gcatacggaa
     3121 agtagatgaa ggcttcctgc ggaaacaaga tgaggggcat cagcgtgcct agcatttgca
     3181 gccacaacaa gagcagggta acgcgcatga acacgccgat gattagccct agcccgatca
     3241 gacattccca agtcgctaaa atcagaatcg aagtttggga cggaaccaac ccgaaggtca
     3301 gaattccgat cgtgcgcgtt gccagctctt gcgcgggact gagtcctgga aaaaacttta
     3361 ggacaccgaa ccagaaaaag acaatgccca gacttattcg caaaagcgta atgccatagc
     3421 gggccatcca atgggtaagt cgcacatcaa ttgctgtaaa taatttgcgg aggctgttca
     3481 tacgatcctc tttgaattaa ggtaattgat cagttcagtg ttgagggata tttgtgaatt
     3541 ctccttggcg tgaaaagtgt tattgcacta cgatttcctg cccgatcatc tgatcaccct
     3601 cgaacaagaa ccgcccgcga aacgatcgat cgctcagcat atgcggaaac caaatccgat
     3661 catcatccat tggagttgta aggcgattgg ctaacgccta actagcggta gatcgatcgc
     3721 gaatggcgaa tactcaactg cgccgatgtc gcaatgtgat ccgatcggtc tgcgccagcc
     3781 ccgctgatcg atcgtgagaa gtgcaccgag atcatcagtg cagccggtgg gtcggcccgc
     3841 gtcgattgcc gggctgccgg gcaggggcga ttgagttaaa gtcgaaccac cgttcagttg
     3901 gagcgggcct agtttggggt caatccccag gacatcgccg atggagggac cttcgaactg
     3961 gcaatgggcc attgtctcaa tcaggttata gcctttggcg cgcactttgc cgtagcaatc
     4021 gtcagacacc ggcgcgttga taccgacgtg gagggcgttg tcgccaatca gcgtgttcgt
     4081 gagggtaatg attgcgggaa tgccatagtt gggcgttatc aaaagcccgg cacccatggc
     4141 gggataagag taccccgccg tcacaacgat c
//
LOCUS       DASRTJ010000334         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_322319,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000334 DASRTJ010000000
VERSION     DASRTJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322319"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="selB"
                     /locus_tag="VFF70_06805"
     CDS             complement(<1..1149)
                     /gene="selB"
                     /locus_tag="VFF70_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZY44441.1"
                     /translation="MRVIGTAGHVDHGKSTLVQALTGTHPDRLKEEREREMTIDLGFA
                     WLTLPGGEAIGLVDVPGHIDFIENMLAGVGGIDATLFVIAADEGIMPQTREHLAILDL
                     LKIDGGVVALTKIDLVDPEWIDLITADIRSALRGSILQDAPIVPVSARTGVGLPDLFA
                     ALDRTLQQTEPRIDRSRPRLSIDRVFSIAGFGTVVTGTLIDGSLNIGDEVTILPKNLP
                     ARVRGLQTHKAKIDQAVPGSRVAINLSGVEVIDLKRGNVVTTPNWLSPTLLIDARFEH
                     LKTSPRPITHNMEVKFFHGAAEVPAHIRLLNDQSIAPGQTTWVQFALREELAVIKGDR
                     FIVRLPSPSITLGGGTVIDPQPGRRHRRFRSDVIARLKTLAQGTPSELL"
     gene            complement(1153..2391)
                     /locus_tag="VFF70_06810"
     CDS             complement(1153..2391)
                     /locus_tag="VFF70_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44442.1"
                     /translation="MELESTQSAQLIKWLEEERRKDKAQISTLQERISGQENAITELT
                     RRFQDLEASLKASQASLVKVMQFEQMLEQYKTDMIGVIDRHDDERKKTERETERVRNI
                     ELEGLQRQWGDVRKELPRFSKIEEDLVGRRSEEKRLSDLVRQLQMQVEAAAQRIDGAT
                     RGIPYLEEGRRQDTRRIAAIEQDLPKQAKKIEAAIGKIQVLEDAISKLPPKVDQMNGR
                     LADQDKVIENIKASGFQVQQQIKLSEADLTKFRMQIAEFSDVSAKVREQSQHNDRARA
                     ELQTFQETLRQRANEGSEVQRLHEERIKRQLEEAVDAEEKRWGTHLAKFSEQWPEHDR
                     LHAKQDERFAALESVPAPLTESIDTLRAEYEKLVKTLFDAVASLRESKRSTLPSVSVS
                     PAYTPDDGGAPAPHRKTKRK"
     gene            2533..3642
                     /locus_tag="VFF70_06815"
     CDS             2533..3642
                     /locus_tag="VFF70_06815"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY44443.1"
                     /translation="MHIALDARLVYYRRYSGIGQYIVQLAEELPALKPDWQFTLIHSR
                     KDRSMASPLARLMPAWTPSHHRFEQIVFPIELRRLKIDLLHSPDFIPPFRGDFKRIIT
                     VHDLNFLYYPQFLTADSRRYYNDQIERAVTLADHILADSHATKNDLIKVLNVPAEKIS
                     VVWLAPNSIFRPIDRAALNEPRSRLQLPDRFILFTGTFEPRKNIAGLLIAYRSLLDRT
                     HESIDLVLAGSRGWLFNETYALIHQLHLTDRVRLIEAPSESDLVGLYNCATVFVLPSY
                     YEGFGLTVLEAMACGAPCIISNRGSLPEVAGHAALIVDPDDEAQLTDALERVLSDPQL
                     RSNLKQQGFERVEEFSWQRCMRETLAVYLRTLKAE"
     gene            3872..>4186
                     /locus_tag="VFF70_06820"
     CDS             3872..>4186
                     /locus_tag="VFF70_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HZY44444.1"
                     /translation="MKFLFLTPHYPYPPNKGTALRNYNIIANLAARHEIDLLTFADVE
                     PPADSPLNTLCRRIATVSAHKRSIVRRAIDTLLSPWPDMGLRLWSIEFRQKLNEWLAA
                     TPY"
BASE COUNT          846 a         1163 c         1128 g         1049 t
ORIGIN      
        1 caacaattca gacggtgtgc cttgggctaa agttttcagg cgggcgatga catccgagcg
       61 gaagcggcga tgtcgccgtc ccggctgtgg atcgatcacc gtgccgccgc cgagcgtgat
      121 cgatggcgag ggtagccgca caatgaatcg atcgcccttg atcacggcca actcttcgcg
      181 caaagcaaac tgtacccacg tagtctgccc cggcgcgatc gattgatcgt tcaataaacg
      241 aatgtgcgcc ggaacttcgg ccgcgccgtg aaaaaatttc acttccatgt tatgcgtgat
      301 cggtcgcggc gaagttttca aatgttcaaa ccgcgcatcg atcagcagag tcggggatag
      361 ccaattcggt gtcgtcacca cattgccgcg tttcagatca atcacttcga cgccgctgag
      421 gttgatcgcc acgcggctcc ccggcacggc ctgatcgatt ttggctttgt gcgtttgcag
      481 gccgcgcaca cgcgccggta agttcttggg cagaatggtg acttcgtcgc cgatgttcaa
      541 tgaaccatcg atcagcgtgc ccgtcacgac cgtgccgaac cccgcgatcg agaagacgcg
      601 atcgattgag aggcgtggtc gactgcggtc gatgcgcggt tcggtttgct gcaatgtccg
      661 atcgagtgca gcgaacaaat cgggcaggcc gacacctgtt cgggcagaga ctggaacgat
      721 cggggcgtcc tgcagaatcg atccgcgcag cgcggagcga atatcggcgg tgatcagatc
      781 gatccattcg ggatcgacta aatcgatttt ggtgagcgcg acgaccccgc catcgatctt
      841 gagcagatca agaatggcta agtgctcgcg cgtctgcggc ataatgcctt catccgcggc
      901 aatgacgaat aaagttgcgt cgatgccgcc aacgcctgct aacatgtttt cgatgaagtc
      961 aatatggccg ggcacatcga cgagaccgat cgcttcgccg ccggggagcg tcagccaggc
     1021 gaaaccgaga tcgatcgtca tctcgcgttc gcgttcttct ttgagccgat cgggatgagt
     1081 gccggtcagg gcttgcacca gcgtcgattt gccgtgatcg acgtggccgg cagtgccaat
     1141 gacacgcatg ctttatttgc gctttgtttt tctgtgcggc gcgggcgcgc cgccatcgtc
     1201 gggcgtataa gcaggtgaga cggaaactga cgggagagtg ctgcgtttcg attcacgtag
     1261 actggcaacg gcgtcgaata aagttttgac caacttttcg tattcggcgc gcagcgtgtc
     1321 gatcgattca gtcagtggcg cgggcacgga ttcaagtgcg gcgaatcgct catcttgttt
     1381 ggcatgcaac cgatcgtgtt ccggccattg ttcactgaac ttggctaagt gtgtccccca
     1441 acgtttttct tccgcgtcca ccgcttcttc gagttgccgt ttgatgcgtt cttcgtgcag
     1501 gcgctgcact tccgaacctt cgttcgcgcg ctggcggagt gtttcctgga atgtttgcag
     1561 ttcggcccgc gcccgatcgt tgtgctgcga ctgttcgcgc actttggcgg atacgtcaga
     1621 gaattcggcg atctgcattc ggaacttggt caggtctgct tcgctcaatt taatttgctg
     1681 ctgcacttga aaaccgctgg ccttgatatt ttcaatcacc ttgtcttggt ccgccaagcg
     1741 gccattcatt tgatcgactt tcgggggcag tttgctgatg gcgtcttcca gcacttgaat
     1801 cttgccgatc gccgcttcga tctttttggc ttgtttgggt aaatcctgct cgatggcggc
     1861 gatgcggcgg gtatcctgac ggcggccttc ttccaggtag gggatgccac gcgtcgcgcc
     1921 atcgatgcgt tgggcggccg cttccacctg catctgaagt tggcgtacca gatcactcaa
     1981 gcgtttttct tcagacctgc ggccaacgag atcttcttca atcttgctga aacgcggcag
     2041 ttctttacgg acgtcgcccc attgacgctg caaaccttcc agttcgatat tgcggacgcg
     2101 ctcggtttcc cgctcggttt ttttgcgttc atcgtcgtgc cgatcgatca cgccgatcat
     2161 atcggtctta tattgctcca gcatctgctc gaactgcatg actttcacca acgatgcctg
     2221 actggctttc aggctggctt ccaaatcctg aaagcgtcgg gtcagttccg taattgcatt
     2281 ctcctgcccg ctgatgcgct cttgcagcgt actgatttgc gctttgtctt tacggcgctc
     2341 ttcttccagc cacttgatca actgcgccga ttgtgtgctt tcgagttcca tagaacggcc
     2401 tcctgactca cgtcgattat acaatggagg atgcatagtg catagtgccg attgcgtagt
     2461 gtagaccgcg tacttcagtg agcaggctgc attaagaaat aggcattaag cattgtgcat
     2521 tataattgac ccatgcacat cgccctcgac gcgcggctgg tctattaccg acggtacagc
     2581 ggtatcggac aatatatcgt tcagctggcc gaagaactgc ctgcgctgaa acccgactgg
     2641 cagttcaccc tcattcacag ccgcaaagat cgatcgatgg cttcaccgct ggcgcgattg
     2701 atgccagcct ggacgcccag ccaccatcgc ttcgagcaga tcgtatttcc gatcgaacta
     2761 cgccgcctca agattgatct actgcacagc ccggatttca ttccgccctt tcgcggcgac
     2821 ttcaagcgca tcatcacggt gcacgatttg aatttcttat actatccaca gttcttgacg
     2881 gctgacagcc gccgctatta caatgatcag atcgaacgcg cggtcacgct ggctgatcat
     2941 attctggccg attcgcacgc gacaaaaaac gatttgatca aggtgctgaa tgttccggct
     3001 gaaaaaatct cagtcgtgtg gctggctccc aattcgatct ttcgcccgat cgatcgggcg
     3061 gcgctgaacg aaccacgatc gcgcttgcag ctgcccgatc gattcatctt gtttaccggc
     3121 acattcgagc cgcgcaagaa catcgccgga ttattgatag cctatcgatc gttattggat
     3181 cgaactcatg aatcaatcga tctggttctt gccggatcgc gtggctggct atttaatgag
     3241 acctatgcac tcattcatca acttcatttg actgatcggg tccgcttgat cgaagcgccg
     3301 agcgaatcag atttagtcgg cctgtataat tgtgcaactg tatttgttct gccatcatac
     3361 tatgaaggtt ttggactcac tgtgcttgaa gcgatggcct gcggtgcacc gtgcattatc
     3421 agcaatcgcg gatcgttgcc cgaagttgcc ggtcatgccg cgttgatagt cgatccagat
     3481 gatgaggctc aattgacgga cgcgctcgaa cgcgtgctat ctgatcctca acttcgatct
     3541 aatctcaagc agcaaggctt cgagcgcgtt gaagaatttt cctggcagcg ttgcatgcgc
     3601 gaaacactgg ctgtttatct acgaacattg aaggctgaat gaccgacgaa aaaagcgcgt
     3661 cattgcgagc tgcgtctgcc tgcctgcatt gtgcagggtt tgcgccgaag caacccttgc
     3721 accgcaatgt cctgcgctgg tgcatccgca ggatgtacca cggagcagtg caggtgtctc
     3781 catgatcatc agctgagagt gcttcgctcg caatgacgtt tgctgaacga tctgtattcc
     3841 gaaatcaaaa atcaaagatc agaaatccag gatgaagttc cttttcctca ctccgcatta
     3901 tccatacccg ccgaacaaag gcacagctct acgcaattac aatatcattg ccaacctggc
     3961 cgcgcggcat gagatcgatc tgctgacctt tgcggatgtt gagccgcccg ctgattcgcc
     4021 cttgaacact ctctgccggc gaattgccac tgtctctgcg cataaacgtt cgatcgtgcg
     4081 gcgggcgatc gatacactgc tctcgccctg gccggatatg ggtttgcgtt tgtggtcgat
     4141 cgaatttagg cagaaactga atgaatggct ggctgctacg ccatac
//
LOCUS       DASRTJ010000335         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_273469,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000335 DASRTJ010000000
VERSION     DASRTJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273469"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFF70_06825"
     CDS             complement(<1..240)
                     /locus_tag="VFF70_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZY44445.1"
                     /translation="MARLFIPGPTDVHPDTLAAQNRSMIGHRGADFEALFAHIQPKLR
                     QVFFTNNRVYISTSSGTGLQEASVRNCVAHKVLCCI"
     gene            complement(298..1095)
                     /locus_tag="VFF70_06830"
     CDS             complement(298..1095)
                     /locus_tag="VFF70_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HZY44446.1"
                     /translation="MNTPHFPARLIFFDCDSTLSSIEGIDELARLKGQFDHIAELTRR
                     AMDGEIKLEDVFAARLDLLRPTRADLRRIAQAYRETIVPDAIETIAALRFAGCQVYIV
                     SGGLLPAVQDFARSLGLPRSHVRAVPIEFDQLAGQWWRYHRNRYSGNADERYLAFAPT
                     PLTETQGKRHIIAELSGSEKRTLLVGDGITDLEARDAVKWFIGFGGVVRRDRVAAEAD
                     IFITAHGLASIVPLALSSEQQEKLKDTGHEAVLERGLDDIEQGLVRF"
     gene            1277..2452
                     /locus_tag="VFF70_06835"
     CDS             1277..2452
                     /locus_tag="VFF70_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HZY44447.1"
                     /translation="MTEPVVPAAPKGLEGVVAASTKMSDVDGNAGELIIGGYRLDAIA
                     GRATFEEIAFVLWNLAMGRPGNLPKRAELDTLQTELNDLRPIPAAALDVIRAAKNAPP
                     MDTLRMAMAMMSLDDPDVKAESLAANYRRAKVLTARTPTIIAAHDRIRRGLEPIAPNS
                     KLNRAGNFLYMLTGNAPHANAAQALDTYWTTVIDHGMNASTFTARVIASTQSDMVSAV
                     TGAIGALKGPLHGGAPGPVLDMLVDIKTIDRAEAWVHTALEHGDRMMGFGHRVYKVRD
                     PRADVLAKTARDLAASGAGDKKLYELATGVEQIILRVLEEVKPGRNLKTNVEFYTALV
                     LQSVGLTPDQFSATFAAGRVAGWTAHVLEQQAENRLIRPASEYIGAKGLKYVPIDQR"
     gene            2532..>3004
                     /locus_tag="VFF70_06840"
     CDS             2532..>3004
                     /locus_tag="VFF70_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY44448.1"
                     /translation="MIRIVTDTTAVLPKEIIGKYQITVVPQVICFGEDSYLEGIEIDE
                     PEFLRRLVLSPTLPGTAAPSPALFDEVYRCAAEHGDTVLSIHPSAEVSGTIESAFVAR
                     SQYPQADIRIIDSRTIAGCLASIVQLAAEWASIGVCADEIADRIQYLIPRARTYF"
BASE COUNT          653 a          777 c          910 g          664 t
ORIGIN      
        1 gatgcaacac aacactttgt gtgccacgca gttgcggacg gaggcttctt gcaggccggt
       61 gccggacgac gttgaaatgt agacgcgatt gttagtgaag aagacttgcc gcagtttggg
      121 ttgaatgtgc gcgaacagcg cctcgaagtc tgcaccgcgg tggccgatca tggatcggtt
      181 ctgtgcggcc aaagtatcag gatgcacatc agtggggccg ggaataaata aacgcgccat
      241 agattctcct tcattgaaaa gatgaccgtt gcgaagcgtc agcgtgtcat cttgtcatta
      301 gaatctaact aagccttgtt caatatcatc aagtccgcgc tccaacactg cttcatgccc
      361 agtatctttt agtttttctt gttgctcgct cgataaagcc agcggcacga tcgaggcgag
      421 gccgtgggct gtgatgaaga tatctgcttc tgctgcaact cgatctcggc gtacgacgcc
      481 cccgaaaccg atgaaccatt ttaccgcatc tcgcgcttcc agatcagtga tgccgtcgcc
      541 gacgagcagc gtgcgttttt cgctgccgct caattcggcg atgatgtggc gcttgccctg
      601 cgtttcggtc agcggcgttg gcgcgaaggc caggtaacgc tcatcggcat taccgctgta
      661 tcgattgcga tgatagcgcc accattgtcc ggccagttga tcgaattcga tcggcacagc
      721 gcgcacatgc gagcggggca gacccaatga tcgagcaaag tcttgaaccg caggcagcaa
      781 tccgccgctg acgatataca cctggcagcc agcaaagcgc agcgcagcga tcgtttctat
      841 ggcgtccggc acgatcgttt cgcgatacgc ctgtgcgatg cggcgcagat cggcgcgcgt
      901 ggggcgtagt agatctaatc gggcggcaaa cacgtcctcc agtttgatct cgccgtccat
      961 cgcgcggcgg gtcagctcgg caatgtgatc gaactggcct ttgagtcgcg ccaattcatc
     1021 aatgccttca atcgaactga gggtgctgtc gcaatcaaag aagatgaggc gagcggggaa
     1081 gtgaggcgtg ttcataaggg tttaccgatc ggcgccatca tatcacggaa atgatgagcc
     1141 atgaaacaga attgacaatt attacttgtc gagtgactca taacaggtta tactcacttt
     1201 agaaagttag ccatgattaa aaactttgcg gccgatgcac catcgtgccg cgtcattttg
     1261 accgagggag attagcatga ccgaaccagt cgtaccggcc gcaccgaagg ggctggaagg
     1321 ggtggtggcc gcatcgacca agatgagcga cgtggacggc aatgcgggcg agttgatcat
     1381 cggcggttat cgcctggatg cgattgccgg ccgtgcgacg tttgaagaga tcgcgtttgt
     1441 attgtggaat ttggcgatgg gtcggcccgg caatttgccc aagcgcgccg aactggacac
     1501 attgcaaact gaattaaatg acctgcggcc gatccccgca gcggcgctgg atgtgatccg
     1561 cgccgcgaag aacgcgccgc cgatggatac attgcggatg gcgatggcaa tgatgtcgct
     1621 tgatgatccg gatgtgaagg ctgaatccct ggcggcgaat tatcgacggg cgaaagtctt
     1681 gacggcgcgc acgccgacaa ttatcgcagc tcacgatcgg atccgccgtg gtctggagcc
     1741 gattgcgccg aattcgaaat tgaatcgcgc gggcaacttc ctgtatatgc tgaccggcaa
     1801 tgcgccgcat gcaaacgccg cgcaggcact ggatacttac tggacaaccg tgatcgatca
     1861 cgggatgaat gccagcacgt tcaccgcgcg cgtcatcgca tccacgcagt cggatatggt
     1921 ctcggcggta accggcgcga ttggcgcgtt gaaaggtccg ctgcatggcg gcgcgcccgg
     1981 cccggtgctg gatatgttag tggatattaa aacgatcgat cgggcggaag cctgggtgca
     2041 tacggccttg gaacatggcg atcgcatgat gggcttcggt caccgcgtgt acaaggtgcg
     2101 tgatccgcgc gccgatgtgt tagccaagac cgcgcgcgat ttggcggcta gtggcgcagg
     2161 cgataagaaa ttatatgaac ttgccacggg tgttgagcag attattttgc gtgtgctcga
     2221 agaagtgaaa ccgggccgca atttaaaaac gaacgtcgag ttttataccg cgcttgtttt
     2281 gcaaagcgtc ggcctcacgc ccgatcaatt ctcagcgacg tttgcggccg gccgcgtggc
     2341 gggctggacc gcgcatgttt tggaacagca agcggagaat cgattgatcc gccccgcgtc
     2401 ggagtacatc ggtgcgaagg ggctgaagta tgtgccgatc gatcagagat aagtaacata
     2461 gtgcggcttg cgtaaggata agacgcggac tacgcacccc gcaacattct gcgggggcat
     2521 tggagtctgt tatgatccga attgtcaccg ataccaccgc agtgcttccg aaggagataa
     2581 ttgggaagta tcagatcacg gtggtgccgc aagtcatttg ctttggcgag gatagttatt
     2641 tagaaggcat tgagatcgat gagccggaat ttctgcggcg attggtcttg tcgccaacgc
     2701 tgcccggcac ggcagctcca tcgccggcgt tgtttgatga agtttatcgc tgcgcggccg
     2761 agcacggcga tacggtctta tcgattcatc cttcggccga agtgagcggc acgatcgaat
     2821 cggcgtttgt ggcccgcagt cagtatccgc aagctgatat tcggatcatc gattcgcgta
     2881 cgattgcagg ctgcttagcc tcgatcgttc agctggcggc tgaatgggca tcgatcggag
     2941 tgtgcgccga tgaaattgca gatcgtatcc agtacttgat tccacgcgca cgcacctact
     3001 tttt
//
LOCUS       DASRTJ010000336        10172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_57730,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000336 DASRTJ010000000
VERSION     DASRTJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57730"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFF70_06845"
     CDS             complement(<1..221)
                     /locus_tag="VFF70_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44449.1"
                     /translation="MFLRFLLYGIGGWCAEILWTSIRDRVRCRVDDWKLKGTTYLWMF
                     PIYGGALLFLYEPAHDAIRSISWPLRGSI"
     gene            complement(272..3397)
                     /locus_tag="VFF70_06850"
     CDS             complement(272..3397)
                     /locus_tag="VFF70_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY44450.1"
                     /translation="MNKRHLILTLLSLLGLAIVAGIAIGIAIDHAQRTRGISYGFPDP
                     TNSPAALIDNGLSVNVTLEQYDETQLAENLKQIKDAGFTWIRQSFPWSQIEATRGQFD
                     WSKWDRIVDTAQGLHLIAVLDTSPSWASTSNFYPPTSNSDFSKFVQAFAQRYGDRIDY
                     YQIWDEPNLRDRWNGEVNPVAYAEMLRQARAAIRQVDPTSLIILAGLAPTIETSSANM
                     ADWLYLRRLYEAGAGSLFDVASGKPYGFGSAPDDHTIDSNVLNFQHIVLMREEMVAHG
                     DASKPIWASHFGWNVADNSIWGEVSDQQQIDYAQRAIRYARANWPWLGVMSIENWEPV
                     AAKDDPHWGFSIKGKKLLEVLYPPGGLGFHPAAIQDRPDDAAYQPNPAAGFTGNWRFS
                     ELGADWSATGDSVSFQFTGTSLALRVRRAADRANLFITIDRLPANALPQDDRGAYLQL
                     IPPDASTTDVRTVPIATGLSDSTHSVLIEAERGWNQWSLIGWSVGSDENRGALVVSYS
                     LLGIGALLFLLGTIFFGRGVQWRTLSKIVSAAYSKLSSGKQVAVALFTSLFLYASAWM
                     TWGVDLSAAYRRTGDVANIIATLAAATIFYVSPWLILTIISGLILFILIIFRLDLGLA
                     LVALFAPFFFLPKELFYSAFSMAELILIMCVIAYVLRAIVPAAKRSRSAWSGRMSPIT
                     HHAALITNYSSRLTSLDCAVASLLIVSIIATFLADYKVFAFRELRVIILEPIVYYALI
                     RSAKLDRQAVWHIVDALILAGTLVAIIGLIQYAFHINIITAEEGTERLRSVYGSPNNV
                     GLFLGRVFPVGLAILLLGNDQRRRILYGLALIPIAAAIVLSQSRGAIFIGLPASILAI
                     GLLAGGKWLWRLIGALVVAGLASIPLLNSPRIQALFNAEGGTSFFRISLWKSALEMIR
                     DHPLFGVGPDNFLYAYRDRYLRPEAWQESALSHPHNFILDFAARLGLIGLGAFVWIQI
                     SFWRTAWFVLRKSKPFDLSHWSLVIGLMASMIDFLTHGLVDAAYFVVDLAYVFMLTLA
                     LVARLRDSEMA"
     gene            complement(3515..5065)
                     /locus_tag="VFF70_06855"
     CDS             complement(3515..5065)
                     /locus_tag="VFF70_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HZY44451.1"
                     /translation="MKFKLGMLIVGAILVAACGPGRQPDQVSITLIADNESHPLLMPQ
                     DQTVRDVLKAQSITLGELDRVRPPETTPLQGGMSITVTRIVQQTQIVTQAVPFTTQII
                     PDTALPSGQRQIQQAGHNGTLQITYRVTYEDGQITSRDEIDRQAIDLPTAEIARVGLQ
                     DFSTTIFSGTLAYLSNHNAFILRGLSTNKRPLTTQGDLDGRVFALSPDGRWLLYSRAV
                     TTTLNALWLVDTTLAKPEARSLKINGVLWANFSPDGKSIAFTRADAAPGEPGWRAYND
                     LNVAAFNAGQLGAAKRIVPQSTTAQYSWWGTTYTWSPDNKSIAYANTESIGVISISAR
                     LTKTTEIGRFAAYNTRSTWAWTPIVSWSPNSRFIVAATHAPSPTNDPNEDSPAFDVNA
                     IASDGSFSVRLINSAGMWSAPRWTTSSLATSKIVFGISDTPFASDVSRYRLFVMDRDG
                     SNHTLLFPAADSIGLDGLPDYDLAPDNRSLIVSYQGDLYIVDIASTTIISRRITAEGT
                     ASHPRWSK"
     gene            5134..6054
                     /locus_tag="VFF70_06860"
     CDS             5134..6054
                     /locus_tag="VFF70_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HZY44452.1"
                     /translation="MTFSIVAFDPIKKEWGVATQSKFLAVGAVVPWAQANIGAVATQS
                     YANTSFGPRGLALMASGLSAAETLDRLLMNDEGREQRQVGVIDRSGSAATFTGRKCME
                     WAGGVIGRGYAAQGNILVSEATVIAIATGFEKANGELADRLVAALAAGQKAGGDRRGQ
                     QAAAVLVVREQGGYAGFNDRYIDLRVDDDRQPIERLAEILQLHHLYFGKPAESELIPM
                     DRSAIRELQRIAKASKQYAGPLSGEYDDVTRSAVEALISTENLEDRWPLGQAKIDPVS
                     FRFLRKLYPPKKKVKRKPVKRSAKPKNKTK"
     gene            6248..6526
                     /locus_tag="VFF70_06865"
     CDS             6248..6526
                     /locus_tag="VFF70_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZY44453.1"
                     /translation="MNTDHQAAVVKRLKSIAGHVNGIERMIVEDAYCIDVIKQVQAVQ
                     AALNKVNDLILENHLNTCVIEAVRGDNKKERERVIGEIVDVFEMAQKI"
     gene            6590..9019
                     /locus_tag="VFF70_06870"
     CDS             6590..9019
                     /locus_tag="VFF70_06870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZY44454.1"
                     /translation="MTTKQITIPITGMTCANCVSTIERVTKKLPGIESATVNLASERG
                     TWNFDPNVLTADQIIHRIKDIGYGVATGEVDLPIQGLHDDADARRVQDAVSKIEGVLK
                     ANVSFATEKASIEYLPTMVGQGDLRRAIEAIGFKAIVAEGASSEDAEAIAREAEFAHQ
                     RRRLIGGLIFTIPLFFLAMLMDAMSANLITLPSALMPFFMWSGLGWLFLALATPVQFI
                     VGYDFYIGAWKALKNKAANMDVLVAMGSSVAFFYSLGVLLNFYAGHIYFETAAVIITL
                     IVLGKYLEAKAKGRTSEALKKLMGLRAKTARIIRDTIELEVSIDDVRVGDTVIVRPGE
                     KIPVDGLVIEGRSNVDESMITGESMPVGKSVGDKVVGATINKQGLLKFEATQVGKNTV
                     LAQIVKLVEQAQGSKAPIQKLADQVSAWFVPMVIIIAAITFAGHLLLDQSAGFTGAML
                     AAVAVLVIACPCAMGLATPTAVMVGTGKGAENGVLFKSSEALERLGKVQIVVLDKTGT
                     ITQGKPTVTDVVVNDKLRMTNDKLLRLAGSIEKVSEHPLGTAIVEAAKDLALAEPIGF
                     QALSGRGVAAEVEGRSILLGNLLLMNEKQVRLNGLIQEVDRLQAEAKTAMVLAVDGEA
                     VGIIAVADTLKAGSKEAIDALHEMGIQVVMITGDNQQTAKAIAQSVGIDRVLAEVLPG
                     DKAAEVKKLQGENKTVAMVGDGINDAPALVQADVGIAIGTGTDIAIEAADVTLMSGDL
                     RGLPKAIMLSRGTLRTIKQNLFWAFFYNIILIPVAAFGALIPMLAAGAMAFSSVFVIS
                     NSLRLRGLKVK"
     gene            9171..9482
                     /locus_tag="VFF70_06875"
     CDS             9171..9482
                     /locus_tag="VFF70_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44455.1"
                     /translation="MKIELLYFEDCPSFKTAAVLLSEALSAAGRSDPIELIEIKDEAD
                     VQRWKFIGSPTIRFGGIDPFDQGTSNFGLECRVFNTPEGLIGWPTKQMLIDAIKLSTR
                     Y"
     gene            9512..9706
                     /locus_tag="VFF70_06880"
     CDS             9512..9706
                     /locus_tag="VFF70_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZY44456.1"
                     /translation="MAIDPVCGMQVDEKTAKNKVEYKGTTYYFCSPGCKFTFNENPED
                     YLGPNRKPFTDMGMHMEHHS"
     gene            9803..>10172
                     /locus_tag="VFF70_06885"
     CDS             9803..>10172
                     /locus_tag="VFF70_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HZY44457.1"
                     /translation="MTKASVRLSERELKRRSSGGQRDLTGWWLLGGAAVISIVLIVVS
                     LRSSSPQIALIDGVVQYDNLARGHSPSPQIYQEVPPVGGTHDPAWLNCGIYDQPVRTE
                     NAVHSMEHGAVWITYQPDLAA"
BASE COUNT         2504 a         2481 c         2943 g         2244 t
ORIGIN      
        1 tagatcgatc cccgcagcgg ccagctgatc gatcgaatgg catcgtgcgc gggttcatag
       61 aggaacagca acgcgccgcc atagatcgga aacatccaca agtacgtggt gcctttcagt
      121 ttccaatcgt cgacacggca acggacccgg tcgcggatcg aagtccagag aatttcagcg
      181 caccaaccgc cgataccgta gaggaggaat cttaggaaca tgttgaatga gggagtggga
      241 gataacgaga atgcgagatt tgctctcgcc ttcacgccat ctcgctgtct cgtaatctgg
      301 caacaagtgc taatgtcaac atgaagacgt aagccagatc aacgacgaag tacgctgcat
      361 caactaaacc gtgtgtgagg aaatcgatca ttgaagccat gagaccaatg accaatgacc
      421 aatgactaag gtcaaatggt tttgatttac gcaacacgaa ccacgcagtg cgccaaaacg
      481 aaatctgtat ccaaacgaat gcacctaagc cgatcaagcc caagcgtgcc gcgaaatcca
      541 aaatgaaatt gtgcggatgc gacaacgccg attcctgcca cgcttcaggc cgcaagtatc
      601 gatcgcgata agcgtacaag aaattgtcgg ggccgacgcc gaataatgga tgatcgcgga
      661 tcatttcgag cgcactcttc cataacgaaa tccgaaagaa tgatgtgccg ccttcagcgt
      721 tgaataacgc ttgaatacgc ggcgagttaa gcaggggaat tgaggcgagg ccggcgacga
      781 ctaaggcacc gatcaatcgc caaagccact tgccacctgc cagtaagccg atcgccaaga
      841 tcgatgcggg caggccgatg aagatcgcgc cgcgtgattg actgagaacg atcgcggcgg
      901 cgatcggaat cagcgcgagt ccgtataaaa tgcgccgccg ctggtcattg cctaacaata
      961 agatcgccaa cccgaccgga aacacgcgcc ccaaaaataa tccgacgttg ttgggcgagc
     1021 catagacgct tcttaatcgc tctgtgcctt cttcggcggt gatgatgttg atgtgaaagg
     1081 cgtattgaat caagccgatg atcgcgacta acgttccggc caagatcagc gcatcgacaa
     1141 tgtgccacac ggcctgtcga tccagtttag cggatcggat gagcgcgtaa tacacgatcg
     1201 gttccagaat gatcacgcgc agttcgcgga aggcgaagac tttgtaatcg gccaagaacg
     1261 tggcgatgat cgagacgatt aataatgaag cgacggcgca atcgagcgag gttaggcgtg
     1321 atgagtaatt agtgattagt gctgcgtgat gcgtgatggg tgacattctg ccggaccacg
     1381 cactcctgct ccgctttgct gcggggacta tcgcacgtaa cacgtatgca ataacacaca
     1441 tgatcaagat caactccgcc attgagaacg cggagtagaa caattctttg ggcaggaaga
     1501 aaaacggcgc gaataaagcc actagcgcaa gaccgagatc gaggcggaag ataatcaaga
     1561 tgaataggat caagccgctg atgatcgtca agatcaacca cggcgaaaca tagaagatcg
     1621 tcgcagcggc caaggttgca atgatgttag ccacatcgcc ggtgcgccga taagccgccg
     1681 atagatcgac gccccaggtc atccaggcag aagcatacag gaatagcgaa gtgaataaag
     1741 cgactgcaac ttgttttccg ctgctgagtt ttgaataggc ggcagagacg atcttgctta
     1801 acgttctcca ttgaactccg cgcccgaaaa aaatcgtgcc gagcaaaaac aataaagcgc
     1861 cgatgcccaa taacgagtaa ctaacaacaa gcgccccgcg attttcatcg gacccaacac
     1921 tccaaccgat cagcgaccat tgattccaac cgcgttcggc ttcgattaga acgctgtgag
     1981 ttgaatcact caggccggtt gcgattggaa ctgttcggac atcagtggtt gaagcgtccg
     2041 gcggaatgag ttgcagatac gcgccgcgat catcttgtgg cagtgcattt gcgggtaatc
     2101 tgtcgatcgt gataaacaag ttcgcgcgat cggcggcgcg acgtacgcgc aaggctaatg
     2161 aagtgccggt gaactgaaat gaaactgaat caccggttgc gctccagtct gcgccgagtt
     2221 cgctaaagcg ccaattgccc gtgaagccag cggcagggtt gggttgataa gcagcatcgt
     2281 ctggtcgatc ttgaatggca gcgggatgga accctaatcc tcccggagga tatagaacct
     2341 ccaggagttt tttgcccttg atcgaaaagc cccaatgtgg atcatctttg gccgcgaccg
     2401 gttcccaatt ctcgatcgac atcacgccga gccacggcca attcgctcgg gcatagcgga
     2461 tggcacgttg ggcgtaatcg atctgctgtt gatcagacac ctcgccccaa attgaattat
     2521 cagctacatt ccagccgaag tgacttgccc agatcggttt actggcatcg ccatgcgcga
     2581 ccatctcttc gcgcatcaac acgatgtgtt gaaagttcaa cacgttcgag tcgatggtgt
     2641 gatcatcggg cgcagagcca aacccataag gtttgcctga agccacatca aataaactgc
     2701 ctgcgccagc ttcgtacaat cggcgcaagt aaagccaatc ggccatgtta gcgctgctgg
     2761 tttcgatcgt tggtgccagc ccggccagaa tgatcagcga agtgggatca acttgcctga
     2821 tcgcagcgcg agcctgacgc agcatctcgg cgtaagcgac tggattcact tcgccgttcc
     2881 atcgatcgcg gaggttgggt tcgtcccaga tttgatagta gtcgatgcga tcgccgtagc
     2941 gttgggcgaa agcttgcacg aatttgctga aatcagaatt ggaagttgga ggatagaagt
     3001 tggatgtgct cgcccaagac ggcgaggtgt ctaagacggc gatgaggtgc agaccctgcg
     3061 ctgtatcgac aatgcgatcc catttgctcc aatcgaattg cccgcgtgtg gcctcgatct
     3121 gcgaccacgg aaaagattgt cggatccaag tgaagcccgc gtctttgatt tgctttagat
     3181 tctcggcgag ttgagtttca tcatattgtt ccagcgttac attgacactc aacccattat
     3241 cgatcaaggc tgctggagaa ttcgtcggat cagggaagcc gtaggagatg ccgcgcgtgc
     3301 gttgggcgtg atcgatcgcg atcccgattg caattccggc gacgattgca agaccgagta
     3361 gggacagcag tgtgagaatc agatggcgct tgttcatatc aatgattgcg ggatagcgtg
     3421 atagcgggag agcgagataa ctctcgtttt ctcgccgtct cgctatccct cattccctaa
     3481 tccctgatta actgatccct cattcactgg tcaactactt tgaccatctt ggatgtgaag
     3541 cggttccttc ggccgtaatt cgccgcgaaa taatagtcgt actggcaata tcgacaatat
     3601 acaaatcgcc ttgatatgac acgatcagac tgcgattatc gggtgcgaga tcgtaatcgg
     3661 gcaagccatc caacccgatg gagtctgcag cgggaaagag cagcgtgtga ttgctgccat
     3721 cgcgatccat tacaaacaat cgataacggc tgacatcgct ggcaaaaggc gtatcggaga
     3781 tgccgaaaac aattttgctc gtggccaagc tgctggttgt ccaacgcggc gcgctccaca
     3841 tgcctgcgga attgatcaag cggacgctga aactgccgtc cgaggcgatg gcattcacat
     3901 cgaaggcggg tgagtcttca ttaggatcat ttgttgggga tggcgcgtga gtggcagcga
     3961 cgatgaaacg actattgggc gaccacgaaa cgatcggcgt ccacgcccag gtcgatcggg
     4021 tgttgtaggc cgcgaagcga ccgatctcag tcgtcttggt caagcgcgca ctgatactga
     4081 tcacgccgat cgattctgtg ttggcatagg cgatcgattt gttgtccggt gaccaagtat
     4141 aggtcgtgcc ccaccaacta tactgcgcag tggttgactg cggcacgatc cttttagcag
     4201 cgcccagttg gccggcattg aatgcggcaa cattgagatc attgtaagca cgccagccgg
     4261 gttcaccggg cgcggcatcc gcacgcgtga aagcgatcga tttgccatcg ggcgagaagt
     4321 tagcccataa tacaccgttg atttttagtg accgcgcctc gggtttggcc aatgtcgtat
     4381 cgacgagcca gagtgcgttg agggtggttg ttacagcgcg tgagtaaagc agccatcgtc
     4441 catcgggcga taaagcaaac acgcggccgt cgagatcccc ctgcgtggtg agcggtcgtt
     4501 tattggtcga tagcccgcgt aggataaacg cattgtgatt tgatagatag gccagcgttc
     4561 ccgagaaaat tgtggtgctg aaatcctgca agcccacccg tgcgatctcg gcggtcggta
     4621 gatcgatcgc ctgtcgatcg atctcatcac ggctggtgat ttgtccatct tcgtaggtga
     4681 cgcgataagt gatctgcagc gtgccattgt gtccggcttg ttggatttgt cgctgcccag
     4741 aaggtagtgc cgtatctgga atgatctggg tcgtgaatgg aactgcttga gtgacgatct
     4801 gagtttgttg tacaatgcgc gtcacggtga tcgacatgcc gccttgcagc ggcgtcgttt
     4861 cgggtggccg gacccgatcg agttcaccga gggtgatgct ctgcgctttt agaacatccc
     4921 gtacggtttg atcttggggc atcaatagcg ggtgtgattc gttatcggcg atcagcgtga
     4981 tcgaaacttg atccggctga cgacccggtc cgcaagccgc cacgaggatc gcgccgacaa
     5041 tcaacatacc caatttgaat ttcatgtggg caatgatacc atagtggcca tgaagaatta
     5101 gcagtgagca atgatcaatg acttaggaga cgcatgacct tttcaatcgt ggcatttgat
     5161 ccgatcaaaa aagaatgggg ggtcgctacc caaagcaaat ttctggccgt cggcgctgtc
     5221 gtgccgtggg cacaagcgaa tatcggcgcg gttgcgacgc agtcatatgc caacacttct
     5281 ttcggcccgc gcggtttggc gttgatggca tccggcctca gcgcagcaga aacactcgat
     5341 cgattgttga tgaatgacga ggggcgtgaa cagcggcagg tgggtgtgat tgatcgatca
     5401 ggcagtgcgg caacatttac tggcagaaag tgtatggagt gggcgggcgg tgtgatcggt
     5461 cgaggctatg cggctcaagg caacattctt gttagtgaag ccacggtgat tgcgattgcg
     5521 acaggctttg agaaagcgaa cggtgagtta gccgatcggt tggtagcggc actcgcggcc
     5581 ggacaaaaag cgggcggcga tcgacgcggt cagcaagccg cggcggtgct ggtcgtgcgc
     5641 gagcagggtg gttatgccgg cttcaacgat cgttatatcg atttgcgtgt cgatgatgat
     5701 cgacagccga tcgaacgttt ggccgagatt ctgcaattgc atcatttgta tttcggtaaa
     5761 cccgctgagt ctgagctgat cccgatggat cgatcagcga tccgtgaatt gcagcgcatc
     5821 gcaaaagcga gtaaacagta cgcaggcccg ctgagcggcg aatatgatga tgtcacgcgc
     5881 tcggcggtcg aggctttgat cagcaccgag aatctggaag atcgttggcc gttaggacaa
     5941 gcgaagatcg atccggtgtc gtttagattc ttacggaaac tatatccgcc aaagaagaaa
     6001 gtgaaacgca aaccggtgaa gagatcggcg aaacccaaga acaaaacaaa ataaactgaa
     6061 agatgtcagg tgcaggttgc atctaacaag tgttcctcgc tgggttgcat tgaccagcgt
     6121 tcattgatag gttcatcctt cttccatccc cttcattcct aagaagtgac attcatcaca
     6181 ttgacagact ggcggtggca tgttacactt tcaccatcta cccccaccgg gggggagggg
     6241 gatatctatg aacacagatc atcaagcagc agttgtcaaa cgacttaaga gtatcgccgg
     6301 acacgtaaac ggcatcgagc ggatgatcgt cgaggatgcg tactgcatcg acgtgatcaa
     6361 gcaggtgcag gcggtgcagg ccgcgctcaa caaggtcaac gatctgatct tggagaatca
     6421 cctcaacacc tgtgtgatcg aagcggtgcg cggcgacaat aagaaagagc gcgaacgcgt
     6481 catcggcgaa attgtcgatg tgttcgagat ggcgcagaag atttaataag tactgagtaa
     6541 tgcgtaaaat gtaaattact cgttactcat cactattcac ttacccacta tgactacaaa
     6601 acaaatcact atccctatta ccggcatgac ctgcgcgaat tgtgtcagca ccatcgagcg
     6661 ggtcacgaag aagctgccgg gcatcgaatc ggcgacggtc aatctcgcca gtgagcgcgg
     6721 cacatggaac ttcgatccga atgtcctcac cgccgatcag atcatccatc ggattaagga
     6781 catcggctac ggcgtcgcga ctggcgaggt cgatctaccg atccagggtt tgcatgatga
     6841 cgccgatgca cggcgtgtgc aagacgcggt cagtaagatc gaaggtgtgc tgaaagcgaa
     6901 tgtatctttc gccaccgaaa aagcctcgat cgaatatttg ccgacgatgg tcgggcaggg
     6961 cgatctgcgc cgcgcgattg aagcgatcgg cttcaaagcg attgtcgccg aaggcgcctc
     7021 gagcgaagac gccgaagcga tagcgcgcga ggctgagttt gcgcatcagc gccgacgatt
     7081 gatcggtggc ttgatcttca ctattccatt gttttttctc gcgatgttaa tggatgcgat
     7141 gtcggcgaac ttgatcacgc tcccgtcggc attaatgccg ttcttcatgt ggagcggtct
     7201 cggttggttg ttcctggcgt tagcgacacc tgtgcaattt atagtcggat atgatttcta
     7261 tatcggcgcc tggaaggcgt tgaagaacaa agccgccaat atggatgtat tagtcgccat
     7321 gggatcgagc gtcgcgttct tttacagcct cggtgtgttg ctgaatttct acgctggtca
     7381 catctatttt gaaacagccg cggtcatcat cacgttgatt gtgcttggaa aatatttaga
     7441 agcaaaagcc aaaggccgca cgagcgaagc cttgaagaaa ttgatgggcc tgcgcgcgaa
     7501 aaccgcgcgc atcattcgtg acaccatcga actcgaagtc tcgatcgacg atgtgcgcgt
     7561 gggcgataca gtgatcgttc gaccgggcga gaagattccg gtcgatggcc tggtcattga
     7621 aggccgatcg aacgtcgatg agtcgatgat cacgggcgag agtatgccgg tcggcaagag
     7681 tgtgggcgac aaagttgtcg gcgcaacgat caataaacag ggtctgctca aatttgaagc
     7741 gacccaggtt ggcaagaaca ctgtcctcgc ccagattgtg aaattggtcg agcaagcgca
     7801 gggatctaaa gcgccgattc agaaattggc cgatcaagtt tcagcgtggt ttgtaccgat
     7861 ggtgatcatc atcgcggcga ttacatttgc gggtcatcta ttactcgatc aatccgctgg
     7921 cttcacgggt gcgatgctcg ccgccgttgc agtgttggtg attgcatgcc cgtgcgcgat
     7981 gggcctggcc acaccgacgg ctgtgatggt gggcacgggc aaaggcgcgg agaatggcgt
     8041 gctgttcaag agcagtgaag cgctggaacg attaggcaaa gtgcaaatcg ttgtcttaga
     8101 taagacgggg acgattactc aaggtaagcc aaccgtaacg gacgttgtcg ttaatgataa
     8161 attacgaatg acaaatgaca agctgttgcg gctggctgga tcaatcgaaa aagttagcga
     8221 gcatccatta ggcactgcga ttgtggaagc ggcgaaagat ttggcactcg ctgaaccgat
     8281 cggttttcaa gccctatcgg ggcgcggggt ggccgcggag gtagaaggcc gcagtatttt
     8341 gctagggaat ttgctgttaa tgaatgagaa gcaggtgcgc ttgaacggac tcatacagga
     8401 agtcgatcga ttgcaggccg aggcgaagac ggccatggtg ttggcggtgg atggtgaagc
     8461 agttggcatc atcgccgtcg cggacacgct gaaagccgga tcgaaagaag cgatcgatgc
     8521 gttgcacgag atgggtattc aagtcgtgat gatcaccggc gataatcagc agactgctaa
     8581 ggcgattgcg cagagcgtgg ggatcgatcg ggtgttggct gaagtattgc ccggtgataa
     8641 agcggctgaa gtgaagaagt tgcaaggtga aaataaaacg gtggccatgg tcggggatgg
     8701 tattaatgat gcacccgctt tagtgcaagc ggatgtcggc attgcgatcg gcacgggcac
     8761 cgacattgcg atcgaggcgg cggatgtgac gttgatgtcg ggcgatctgc gcggactgcc
     8821 caaggcgatc atgctgagcc gcggcacgtt gaggacgatc aaacagaatt tgttctgggc
     8881 gttcttttac aatattattc tgattccggt ggctgccttc ggcgcgctga ttccaatgtt
     8941 ggccgcaggc gcgatggcgt tcagcagtgt gtttgttatc agcaactcat tgaggctgcg
     9001 cggactgaaa gtgaaatgag gtaactgctc agttgtcatg cccgaacgtt tttatcgggc
     9061 atccagattg actgttggcg agttccgctg atagaaactc tggattcccg cctaaagcct
     9121 gcgggaatga cctgcggcaa ccaggcttag cagctacgaa atgaggttga atgaagatag
     9181 aattacttta ctttgaggat tgtccttcat ttaagacagc agccgttttg ttgagcgagg
     9241 cgttgagtgc agcaggccga tcggatccga tcgagctgat cgagatcaaa gatgaagcag
     9301 acgttcagcg ttggaaattt atcggctcac caacgatccg ctttggcggc atcgatccat
     9361 tcgatcaagg tacatcgaac tttgggctgg agtgccgcgt gttcaatacg ccggagggct
     9421 tgatcggctg gccgacgaag cagatgttga tcgacgcaat caaactctcg acgcgctact
     9481 gagccgcgtc gaataatcaa ggagaaagaa aatggcaatc gatcccgtgt gtggcatgca
     9541 agtcgatgag aagacggcga agaacaaagt ggaatacaaa ggcaccacgt attacttctg
     9601 ctcgcccggt tgcaagttca cgttcaatga gaacccggaa gattacctcg ggccaaatcg
     9661 caagcctttc accgatatgg gcatgcacat ggaacatcat tcataacttc gcgcgaactc
     9721 gcattgaacc gatgggcaga atatcactca tcggttcaat gcgttataat tggcctgatc
     9781 acttttaagg gagaagaatt aaatgaccaa ggcatccgtc cgattgtcag agcgtgaact
     9841 gaagcgccgg agcagtggcg gccagcgaga cctgacaggc tggtggctgt taggcggcgc
     9901 cgccgtgatt tcgattgttt tgattgtagt atcgctccga tcgtcttcac cgcaaattgc
     9961 gctgatcgat ggtgtggtgc agtacgataa tttggcgcgc ggacatagcc cttcgccgca
    10021 gatttatcag gaagtaccgc ccgtcggtgg aacacacgat ccagcttggc tcaactgcgg
    10081 catctacgat caaccggtgc gcacggaaaa cgcggtgcat tccatggaac acggcgcagt
    10141 ctggattact tatcaaccgg atttggccgc gg
//
LOCUS       DASRTJ010000337         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_322623,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000337 DASRTJ010000000
VERSION     DASRTJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322623"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VFF70_06890"
     CDS             complement(<1..1843)
                     /locus_tag="VFF70_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44458.1"
                     /translation="MKRFLIILLALFLLGACSNPQPAQPIAPSLVPSIPPSIVPQPSP
                     TPDTSTWLPALEATIDLGTVIGEPYFMAHPIAIDSKNNRLYIATAISQTLVLDAKTFQ
                     KIDEITIGGNLSLSSDRLYIGVPGAYDSFGKQISTSELRAYDLPTLKLSLHLFYTDTS
                     LLPAYVLVNEAQQKIYVVRSGVFEADPKTLRVLGSISGTVPIKNGLIPNYSAVDAAID
                     PVHNRLIASLNNGIPGSNGGNGLAIFDLTNNTLLATDDERSVQSLDVNPVLGESYVVR
                     SFTGSRSIAKYDQHGQLLQRLDGMTGAVQIDAAHRRVYVFEAYPRARLVILDADLNYQ
                     GEMRFAGLDKVVAFQFDAQNDRVLMLARTGKLFVLKGHATPDQPTATATTAHGSIQWL
                     VPSPDYANDHNLFAAFAATDYVVGAGAVFHSMDNGKTWQAINGLPMSDTVSAVAFSSN
                     YASDHTLYAALGSSDRLPGDGSGVFRSADEGQTWGVASHGLTDLAINQIVAADAQTLF
                     AVGTARGLFRSIDRGQTWTALADRYVREDSYASPILNALVVSPDYTHDKTVLISGLSG
                     GLQTSHDGGETWRLTAPGGISHLSYLSNGNILAALQSGGVIRSTDNGD"
     gene            complement(1935..>2433)
                     /locus_tag="VFF70_06895"
     CDS             complement(1935..>2433)
                     /locus_tag="VFF70_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44459.1"
                     /translation="YGSMRFVAYAVPNILSDKPTQLSVARFGDHIILDGYTLSTPLLA
                     PGDILQLDLYWHTDAVLNDRYKVFVHVLDPNGNIVAQTDREPGGGLVPTTIWQPNQTV
                     IDRYGVLIPADTSAGNYSIEVGLYDFNNIRLKIDAGRDALNLERIVIMSPSTSLRVNS
                     AMQSQ"
BASE COUNT          595 a          684 c          621 g          533 t
ORIGIN      
        1 gatcaccatt gtcggttgac cggatcaccc cgccactttg cagtgcagcc aaaatatttc
       61 cgttcgacaa ataactcaag tgcgatatgc cgcccggcgc agtcaatcgc caggtctcgc
      121 caccgtcgtg tgatgtctga agaccgcccg acaaaccgct gatcagcacg gttttgtcgt
      181 gagtataatc gggggaaacc accaatgcgt ttaagatcgg actggcatag gaatcttcgc
      241 gcacatatcg atcagctaaa gccgtccacg tttgtccgcg atcgatgctg cgaaacaagc
      301 cgcgcgccgt gccaactgca aataaagtct gcgcatccgc cgctacgatc tgattgatcg
      361 cgaggtcggt caaaccgtgt gaggccacac cccaagtctg accttcatct gcgctgcgaa
      421 atactccact gccatcgccg ggcagcctgt cgctcgagcc gagagccgca tataaagtgt
      481 gatcactggc gtagttcgaa gaaaaggcca cagccgaaac ggtatcactc atcggcagcc
      541 cgttgatggc ctgccaggtt ttaccgttat ccatgctgtg gaacacagcg cccgcgccca
      601 ccacataatc ggttgctgca aacgcagcaa ataaattgtg atcattagcg taatccggcg
      661 aaggcaccag ccactgaatc gatccgtgcg ccgtcgtcgc cgttgcggtt ggttgatccg
      721 gtgtggcatg gcccttcagc acaaataatt ttccggtgcg cgccaacatc aacactcgat
      781 cattctgtgc atcaaactga aacgcaacga ccttatcgag cccggcgaag cgcatctcgc
      841 cttgataatt gagatcggca tcgaggatca ccaaccgcgc acgcgggtac gcttcaaata
      901 catacacgcg ccgatgcgcc gcatcgatct gcaccgcgcc cgtcatacca tccagccgct
      961 ggagcagctg gccgtgctga tcgtatttgg cgatcgatct tgaaccggtg aagctgcgca
     1021 ccacatacga ctcccccaac accggattaa catcgaggct ctgcacgctg cgttcgtcat
     1081 cggtcgctaa cagggtattg ttagtcagat cgaagatcgc caggccgttg ccgccgttcg
     1141 atccgggaat gccgttattc agactggcaa tcaatcgatt gtgcaccgga tcgatcgccg
     1201 catcgactgc cgaataattc gggatcagcc cgttcttgat tggcacggtg ccgctgatcg
     1261 atcctaagac tctcagggtt ttcgggtcag cttcaaaaac gcccgatcga acaacataga
     1321 ttttctgctg cgcctcatta accagcacat acgccggcaa cagcgaagta tcagtataaa
     1381 aaaggtgcag cgacagttta agtgtcggca gatcgtaagc ccgcaattcg ctcgtcgaaa
     1441 tctgtttgcc gaaagaatcg tatgcgcccg gcacgccaat atacaatcga tccgaactca
     1501 ggctgagatt gccaccaatg gtgatctcat cgatcttttg gaacgttttg gcatctaaga
     1561 ccagcgtttg cgagattgca gtcgctatat acaaccgatt gttcttcgaa tcgatcgcga
     1621 tcggatgcgc catgaaatac ggctcaccaa tcaccgttcc cagatcgatc gtcgcttcca
     1681 gcgccggcag ccacgtcgag gtatcgggtg tgggtgaagg ctgtggaacg atcgacggcg
     1741 ggatagaggg cacgagagat ggcgcgatcg gttgtgcggg ttgcgggtta ctgcaagcac
     1801 ctagtaagaa gagagcaagc aggatgatta aaaagcgttt catagtaact ccattccaat
     1861 ttaacgaggc atcctcagac cgacatatca ttgcgaggag cgccgtgcgc gacgaagcaa
     1921 tctctcttcg atcatcattg agattgcatc gcggagttta ccctgagcga agtcgaaggg
     1981 ctcattatga cgatccgctc gagattcaac gcatctctcc ctgcatcgat cttcaaacgg
     2041 atgttattga aatcatacaa cccaacctcg atcgagtaat tccccgccga tgtatccgcc
     2101 ggaatcagca cgccataccg atcgatcaca gtctgattcg gctgccagat cgtcgttggc
     2161 acaagtccac cgcctggctc gcgatcggtt tgggcaacga tgttgccatt tggatcaagc
     2221 acatgcacga aaactttgta tcgatcattt aatactgcgt cggtatgcca gtaaagatca
     2281 agttgtagaa tgtcgcccgg tgcaagcaac ggcgttgata acgtatatcc gtcgaggata
     2341 atgtgatcgc cgaagcgagc aacgctgagt tgagtaggct tgtcagataa tatattcggc
     2401 acggcgtacg ccacgaagcg catcgatcca tac
//
LOCUS       DASRTJ010000338         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_322637,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000338 DASRTJ010000000
VERSION     DASRTJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322637"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFF70_06900"
     CDS             <1..292
                     /locus_tag="VFF70_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZY44460.1"
                     /translation="DRPEVCKSADLLAPEGYGEIIGGSERISDPEKLAQRIHDHQLPV
                     EPYQWYIDLRKYGSVPHSGFGLGVERTVSWICGLEHIREVPPFPRLINRVSP"
     gene            complement(373..615)
                     /locus_tag="VFF70_06905"
     CDS             complement(373..615)
                     /locus_tag="VFF70_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44461.1"
                     /translation="MAVRRELNETYLIRGNGGIQAVQGRLLQHGLCSFTTWDPSPPQW
                     RFVFDTYLSKYELDQMLIDLARRYDVRVELKKQTGM"
     gene            complement(686..1582)
                     /locus_tag="VFF70_06910"
     CDS             complement(686..1582)
                     /locus_tag="VFF70_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY44462.1"
                     /translation="MFYIFLSLTIFLLLILIVAYLGSNYLIARRKPDPATSPKDYGLD
                     FEEVSFISGDGIKLGGWYIPAMNSDRTIIFCAGANGSIDPDVIYAPWFQAANFNVLMF
                     DWRAHGRSAGSVTTLGYHERYDLIAAVEFAKSKGARRIGVLGFSMGGAVAVSTAAVTP
                     SIDAVAIDGAFVHVLSAVAGGLIERGLANWLAYSLARLFLIMTNGRLGINLFEIDPVR
                     WIDRIAPRPLLLIYGDRDPYVSKSEIDQLADRAKAPKEIWRVAEAAHRNIHVLRPEEY
                     RIKLVQFFDQNLQPAQRNDPKF"
     gene            complement(1656..>2031)
                     /locus_tag="VFF70_06915"
     CDS             complement(1656..>2031)
                     /locus_tag="VFF70_06915"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZY44463.1"
                     /translation="GVGTAEDFFEAVARGIDIFDCVLPTRLARHGAALTHIGRINLRN
                     QSLAEESAPIEAGCACYACQNFSRAYIRHLMVAKEIVGLYLASIHNLHFLLDTMKQIR
                     QSIFDETFEQLKAEMMPMLMSE"
BASE COUNT          511 a          558 c          519 g          443 t
ORIGIN      
        1 ggatagaccg gaagtgtgca agagcgcgga cttgctcgcg cccgaagggt acggcgaaat
       61 catcggaggt tcggaaagaa tttcggatcc tgaaaaatta gcgcagcgca ttcacgatca
      121 tcaactgccc gtcgaaccgt atcagtggta tatcgatctg cgcaagtacg gcagcgtgcc
      181 gcattccggt tttggcttgg gcgtcgaacg caccgtctcg tggatttgcg gactggaaca
      241 catccgcgaa gtgccgccct tcccccggtt gatcaatcgg gttagcccct aaacccgatc
      301 ggttgttcat cgacaaaaaa agcggcgaag ccaaatgctc cgccgctttg ctcctcttct
      361 tcatcaaggt ttctacatgc cggtctgctt cttcaattca acgcgcacat cataacggcg
      421 cgcgagatcg atcagcatct gatccaattc atatttggac agatacgtgt caaacacaaa
      481 acgccattgc ggcggactgg gatcccacgt agtgaagctg cataagccgt gctgcaatag
      541 ccgtccctgc accgcctgaa tgccgccgtt gccgcgaatc agataagttt cattgagctc
      601 gcgccggact gccatatcct gatcatagat tagcttgcca agcaagtcaa gcgtattgcg
      661 gttacagttg aattaaaccg tgaagttaga atttcggatc atttcgctgg gcaggttgca
      721 aattttgatc aaagaactga acaagtttga tccggtattc ttccggtcga agcacatgaa
      781 tgttgcgatg cgccgcttcg gcgacgcgcc aaatttcttt cggtgcttta gcccgatcgg
      841 ctaactgatc gatttcggat ttcgacacat acggatcgcg atcgccatag atcaataaca
      901 gcggcctcgg cgcaatccga tcgatccagc gtaccggatc gatctcaaag agattgattc
      961 ccaagcgccc attggtcatg atcagaaaga ggcgcgctaa agaataagcc agccaattcg
     1021 ccagcccgcg ttcgatcaag ccgccggcga ctgcgctcag cacatgcacg aacgcgccat
     1081 cgatcgccac ggcatcgatc gagggtgtga ccgcggctgt cgataccgcc accgcgccgc
     1141 ccatcgaaaa gcccagcacg ccgatccggc gtgcgccttt tgacttggca aactcgaccg
     1201 ccgcgatcag atcatagcgc tcatgataac caagtgtcgt aaccgatccg gcgctgcggc
     1261 cgtgcgcgcg ccaatcgaac atcaagacgt tgaagttagc cgcctgaaac cacggcgcat
     1321 agatcacgtc cggatcgatc gatccatttg cgcccgcgca gaaaatgatc gttcgatctg
     1381 aattcatcgc cggaatatac cagccgccga gtttgatccc gtcgcctgaa atgaagctca
     1441 cctcttcaaa gtcaaggcca taatctttcg gcgaggtggc cggatcgggt ttgcgtcgcg
     1501 cgatcagata attcgagccg aggtaggcga caattaaaat gaggagcaga aaaatggtaa
     1561 gcgagaggaa aatgtagaac acggtgtgat gaatggaaaa taattagtgg ttagtaatta
     1621 gtgagtagag cgtgccatct actatttact aatcactatt cactcattag catcggcatc
     1681 atctcagcct tgagctgctc aaacgtctcg tcgaagatcg attggcgaat ttgcttcatt
     1741 gtatcaagca ggaaatgcaa gttgtggatc gaggccagat acaagccgac gatctctttc
     1801 gccaccatca aatgtcgaat gtaagcgcgg ctaaagttct ggcaggcgta acacgcacag
     1861 cccgcttcaa tcggcgcact ctcttcggct aatgattgat tccgcagatt gattcgaccg
     1921 atatgcgtca gcgctgcacc gtgacgcgcc aagcgcgtgg gtaagacaca atcaaaaata
     1981 tcgatgccgc gcgccaccgc ttcaaaaaaa tcttcggcgg tgccgacacc c
//
LOCUS       DASRTJ010000339         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_19979,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000339 DASRTJ010000000
VERSION     DASRTJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19979"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="murF"
                     /locus_tag="VFF70_06920"
     CDS             <1..1172
                     /gene="murF"
                     /locus_tag="VFF70_06920"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZY44464.1"
                     /translation="RAQQPIGLSSLPIVLRVEQSLIALQDLSKWWRNRFKPLRVIGIT
                     GSVGKTSTKELTAAVVQQDFETLKSEGNLNNEIGLPLTLLQLESQHQRAVLEMGMYAR
                     GEISRLAELAQPIIGVVTNVGPIHLERMGTIEAIAEAKAELVQALPADGTAILNWDDP
                     RVREMASLTHARVLSYGLDPLADVWADEIASEGLEGIHFTLHHRREALHVQVPLLGRH
                     SVHTALRAAAVGLAEGMAWDHILAGLQDRRAPLRLVATAGPQESIILDDTYNANPQST
                     IAALNLLADLTGRKIAVLGDMLELGAYEAVGHRMVGLRAIEVADVLVTVGELGQLIAA
                     EAVQNGMPSDRVQACGTNAEAVAYLEKIIEPKDVILIKGSRGLHMEDIVTALDRA"
     gene            1184..2245
                     /gene="mraY"
                     /locus_tag="VFF70_06925"
     CDS             1184..2245
                     /gene="mraY"
                     /locus_tag="VFF70_06925"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZY44465.1"
                     /translation="MAVALLLGGLSFLLAVVWGPPLIRLLRAKGIGKQIRIEGPQTHQ
                     VKAGTPTMGGLMIIVPVVVITAVMVIISAIAEAKRGEPIANIGFAILVPMGTLVAYGF
                     LGAIDDLANVRGTRVKGEGMLGRYKLAIQIGLATIIALVLHFSPSTQLHSVAIPGIAQ
                     KIDIGLVWIPIAVFWIVGFSNAVNLTDGLDGLAGIIMATAFLAYAVIAYLQSFAGAAP
                     GVQPPVSSLVPFSMIMVGAVLAFLWFNAHPAQLFMGDTGALALGAALAVVALMTGQWL
                     LLPVIALVPVAETLTVIFQVAYFKLTKRRYGEGRRLFKMAPLHNHFELLGWSETQIVQ
                     RLWLIGIWAAMLGMALALL"
     gene            2267..3637
                     /gene="murD"
                     /locus_tag="VFF70_06930"
     CDS             2267..3637
                     /gene="murD"
                     /locus_tag="VFF70_06930"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZY44466.1"
                     /translation="MSLEIRDKRFVVLGLARQGSALARWLTAQGAQVTISDAKPIDQL
                     QSEIDALQGVDVNYALGGHPIDLLADCDVLCLSGGVPIDLPIVLAAKERSIPLTNDAQ
                     LFVERCPAPIAGITGSAGKTTTTTLVGAMSKTAGFSTWVGGNIGNPLISDLETITPAD
                     KVIMELSSFQLELMTTSPQVAAILNITPNHMDRHASMDAYIAAKRHILDYQSLEDTAV
                     LGYDDLTARKLSGMARGYVRFFSREKTIGRGAFVKGNRVVLVQNQEIDVCGLNDIQLR
                     GAHNVLNVLAACAIASAMNIDPEVIAGGIRNFTGVEHRLQLVREWNGVKFYDDSIATA
                     PERLMAGLQAFSEPIVLLAGGRDKHLQWQAAARLIVDRVEQLILFGEMADLLQNAVEI
                     ELRSRSGSLIQIQRVATLDEAVQHAASVARSGDVVLLSPGGTSYDAYKDFAERGDMFQ
                     ELVLRL"
     gene            3849..5063
                     /locus_tag="VFF70_06935"
     CDS             3849..5063
                     /locus_tag="VFF70_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HZY44467.1"
                     /translation="MAQLLQVQKSKIKNQRSEIGSLPRADYPLIAAVGALLVIGLMMI
                     YSASYEVAYQTKGDAAYYVLRQAVFTLGGIVVAVLLARSDFSSWRRWSVLLLGAILIL
                     LIVVRLFGADLYGAQRSFFDGSVQPSEIAKLVVILYIADWLASKGDKIRDLNYGLIPF
                     AAIVGGVAGLIIIQPDYGTAILIVLIAGAMFFMAGADLKQFFFSVLIAGLIIGGMVLL
                     SPHSHIRLDDYMKGLQDPALASDQLRQAIRAMQIGGISGVGLGNGLVKVGFLPLAHTD
                     TIFGLIAIETGFFGVLALLSLYAIVAYRGFRIAMTASNTYGQLLAVGATSYIILQALI
                     NVNVMTGMLPLTGIPLPFVSYGGSSLISVMAAVGILISVSRGSRKGKTYGAFMDRSRR
                     DGRSRLPRISRR"
     gene            4999..6129
                     /gene="murG"
                     /locus_tag="VFF70_06940"
     CDS             4999..6129
                     /gene="murG"
                     /locus_tag="VFF70_06940"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZY44468.1"
                     /translation="MRLWIAAGGTGGHVYPALAVVEKIRDQGSGISDQGSRSDPEQSE
                     ILWIGGHGGVEENLVARANIPFIAIPAGGLHGVVIWSAVRNLIKLIAGTVKSMKLIGQ
                     YQPSVMFTTGGFVAAPVAVACWLRRVPIVAYLPDIEPGVAVKFVARLAKKIGVTTADS
                     ITYFRSNKVVVTGYPTRSDFVTADRATALDRFELDPNRKTILVFGGSKGARSINRAIG
                     SILPVLLAKYQIIHVSGSTDALEVQALRDQLPAEVRRRYHVYEYLHDMGLALAAADLA
                     VSRAGASILGEYPLFGLPSILIPYPYAWRYQTVNADYLASQGAAIRLMDDETLSAKLS
                     IEIDRLMNDAVTLQAMRAAARSLAQPDPAERLADVIRSVSRA"
     gene            6126..>6644
                     /locus_tag="VFF70_06945"
     CDS             6126..>6644
                     /locus_tag="VFF70_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44469.1"
                     /translation="MNALINANDLIIPEIAILGLISIAAAAWGYRHGLDTVLITAVFV
                     VFGRVSSDILAVPVAIILNVLYGFFSLIASGKFSRDALTSSIFGGENGPHLIDTSNTG
                     DQIFVLISTVIFIVVCYIGVRFAVKKAGGKDPLIEAVFGAIGGGAMAYLTFGYVINRV
                     VSLPANIKITATD"
BASE COUNT         1359 a         1593 c         2068 g         1624 t
ORIGIN      
        1 atcgtgcgca gcagccgatc gggcttagct cacttccgat cgtgctgcgc gttgaacagt
       61 cgctgatcgc gctgcaagat ttgtcgaagt ggtggcgcaa tcgattcaag ccactgcgcg
      121 tcatcggcat tacgggcagt gtcggtaaaa ccagcaccaa agaattgacg gccgcggtcg
      181 tgcagcaaga ttttgaaacg ttgaaaagcg aaggcaattt gaacaatgag attggtttgc
      241 ctttaacgct cttgcagtta gagagtcaac atcaacgcgc cgtgttggaa atgggcatgt
      301 acgcgcgcgg tgaaatttcc cgactggccg aactcgctca gccgatcatt ggcgtggtta
      361 cgaacgtagg accgattcac ctggagcgca tgggcacgat cgaagcgatt gccgaggcga
      421 aagcagaatt agttcaggcg ctgcccgcgg acggcacagc tattttgaat tgggatgatc
      481 cacgtgtgcg tgagatggcc agtctgactc acgcgcgggt gttgagctac ggactcgatc
      541 cgctggccga tgtgtgggct gacgagattg cgagtgaagg cttggagggt attcacttca
      601 cgctgcatca tcgacgcgaa gcgctgcatg tgcaagttcc attgttggga cggcacagcg
      661 tgcacacggc gctgcgcgcg gcggcggtcg gtttagcgga aggcatggcg tgggatcaca
      721 ttctggccgg cctgcaagat cgacgcgcgc cgctgcgcct ggtggccacg gccggcccgc
      781 aagaatcgat cattctggac gatacctaca atgcgaatcc gcaatcgacg attgcggctt
      841 tgaatttgtt agcggatctg actggccgca agattgcagt gttgggcgat atgctggaac
      901 tcggcgcgta tgaagcggta gggcatcgca tggtgggtct gcgcgcgatc gaggtggctg
      961 atgtgctggt gaccgttggt gaactcggcc aactgatcgc agcggaagcg gtgcagaacg
     1021 gaatgccaag tgatcgagtg caagcgtgcg gcacaaatgc cgaagcggta gcttatctgg
     1081 aaaagatcat tgagcccaaa gacgtcatct tgatcaaagg ctcgcgcggc ttgcacatgg
     1141 aagacatcgt caccgcgctg gatcgtgcct aaaaggaagc gacttggctg ttgcactgtt
     1201 gctaggagga ttatcgtttt tactggctgt ggtctggggt ccgccgctga ttcgtttgct
     1261 gcgcgccaaa ggcatcggca agcagattcg aattgaaggc ccgcagacac atcaagtgaa
     1321 agcgggcaca ccgacgatgg gtggtttgat gatcattgtg ccggtggtcg tgatcacggc
     1381 ggtgatggtg atcatcagcg cgatcgcgga agcgaagcgc ggcgagccga tcgccaatat
     1441 cggtttcgcc attttggtgc cgatgggtac gctggtggct tacggtttct taggcgcgat
     1501 cgatgatctg gcgaatgtgc gcggcacgcg cgtcaaaggt gaaggaatgc tgggccgcta
     1561 taagctggcg atccagatcg ggttggcgac gatcatcgcg ctggtgctgc acttttcgcc
     1621 atcgacgcaa ttgcacagtg tcgcgatacc gggcattgcg cagaagatcg atatcggcct
     1681 ggtgtggatt ccgatcgcgg tcttttggat cgtcgggttt agcaatgccg tgaatctgac
     1741 ggacggcctc gacgggttag cgggcatcat catggctacg gcttttttgg cctatgccgt
     1801 catcgcttac ttgcaatcat tcgccggcgc agcacccggc gtgcagccgc ccgtttcgag
     1861 tttagttcca ttctcgatga tcatggtcgg cgctgtgttg gcgttcttgt ggtttaacgc
     1921 gcatcccgcg cagttgttca tgggcgatac cggcgcgttg gccttaggcg cggccttagc
     1981 ggtcgtggcg ttgatgacag gtcaatggtt gttgctgccg gtgatcgctt tagttccggt
     2041 cgcggagaca ttgacggtca tctttcaagt ggcgtatttc aaattgacca agcggcgtta
     2101 cggtgaaggc cggcggttat tcaagatggc gccgttgcac aatcactttg aattattggg
     2161 ctggagcgag acgcagattg ttcagcggct gtggttgatc ggaatctggg cggcgatgtt
     2221 gggcatggcc ttggcgttac tgtagatgac gatcgacgaa tgatccatga gtttagaaat
     2281 cagagataaa cgattcgtcg ttctgggctt ggctcgacag ggtagtgcgc tggcgcgttg
     2341 gctaacggcg caaggtgcgc aggtgacgat cagtgatgcg aagccgatcg atcaactgca
     2401 gagcgagatc gatgcgttgc agggtgttga tgtgaattac gcgctcggtg gacatccgat
     2461 cgatctactg gccgattgcg atgtgttgtg tttaagcggc ggtgtgccga tcgatctccc
     2521 gattgtgctg gcggcgaaag agcgatcgat cccgttgacg aatgatgcgc agttatttgt
     2581 agagcgttgc ccggctccga tcgccggtat caccggctca gcgggcaaaa cgacgacgac
     2641 gactttagtg ggtgcgatgt ctaaaacggc aggcttcagc acctgggtgg gcggcaacat
     2701 cggtaatccg ttgattagcg atcttgaaac aataacaccg gccgataaag taattatgga
     2761 actttcgagt tttcagttgg aattgatgac gaccagtccg caagtggcgg cgatcttgaa
     2821 tatcacgccg aatcacatgg atcgccacgc gtcgatggac gcgtacatcg cggcgaagcg
     2881 gcacattctg gattatcagt cgctcgaaga taccgctgtg ctgggctacg acgatctaac
     2941 ggcgcgcaaa ttgtccggca tggcgcgcgg ctacgttcga tttttcagcc gtgaaaaaac
     3001 gatcgggcgt ggggcgttcg ttaaaggcaa tcgagtggta ttagtgcaga atcaagagat
     3061 cgatgtttgc ggcttgaatg atattcaact gcgcggcgca cacaacgtgc tgaatgtgtt
     3121 ggccgcgtgc gccatcgctt cggcaatgaa cattgatcca gaagtgattg caggtgggat
     3181 tcgaaatttt accggcgtgg aacatcgttt gcagttagtg cgggagtgga acggcgtgaa
     3241 attttacgat gacagcattg cgactgcgcc ggagcgattg atggcgggct tgcaggcgtt
     3301 cagtgagccg atcgtgttgc tggcgggcgg gcgggataag cacttgcagt ggcaggccgc
     3361 ggctcgcttg attgtcgatc gggttgagca gctgatcctg tttggtgaga tggccgattt
     3421 gttgcaaaac gcggtcgaga tcgagcttcg atcgcgttcg ggatcgttaa ttcaaattca
     3481 gcgtgtggca acattagatg aagcagttca gcacgctgcg tctgtcgcac gatcgggcga
     3541 tgtcgtcttg ttgtcgcccg gtggcacatc gtatgatgcg tataaggact ttgcggagcg
     3601 cggcgatatg tttcaagagt tggtcttaag gttataaccc ccaccctgac ccttccccgt
     3661 aataacagga gagggtactg cggtgaggtt tatgggtgtt ggctcaccgc tggaatgccg
     3721 atcaagcgat cggcattcca gcggcggcca cagtgaagaa atatcaatta accaggcgca
     3781 atgtcactga acaattcatg gtttgattcc agagactgac caagtctaaa aacaagcaga
     3841 gattcaagtt ggcgcaacta ctacaagttc aaaaatcaaa aatcaaaaat cagcgatctg
     3901 aaattggatc actgccgcgg gccgattacc cgctaattgc tgcggtcggt gccctgctgg
     3961 tcatcggttt gatgatgatc tatagcgcca gctatgaagt ggcttatcaa accaagggcg
     4021 acgcggctta ctacgttttg cggcaggccg ttttcacgct tggcggcatt gtcgtcgccg
     4081 tgttgttagc ccgcagcgat ttcagcagtt ggcggcgctg gtcggtgttg ctgctgggcg
     4141 cgatcttgat tctgttgatc gtcgttcgac tctttggcgc tgatctgtat ggcgcgcagc
     4201 gatcattttt tgatggctct gtgcagccca gcgagattgc caagctcgtc gtcattcttt
     4261 atatcgccga ttggctagcc tctaaaggcg acaagattcg cgatctcaat tacggtctta
     4321 ttccttttgc tgcgatcgtc ggaggggtgg cgggacttat catcattcag cccgactatg
     4381 gcacggcgat cttgatcgtc ttaatcgcag gcgcgatgtt tttcatggcc ggtgcggacc
     4441 tcaaacaatt tttcttctcg gtgctgattg cgggcttgat catcggcggg atggtgctgc
     4501 taagcccgca ctcgcacatc cgcctcgatg attatatgaa aggcttgcaa gatcccgcac
     4561 tggctagcga tcaactacgg caagccattc gcgccatgca gatcggcggc atatccggcg
     4621 tgggactggg taacggttta gtgaaggtcg gtttcctgcc gttggcacac accgatacga
     4681 tctttggatt aattgcgatc gaaacgggtt tctttggtgt cctggcttta ttaagtttgt
     4741 acgccatcgt cgcatatcgc ggctttcgca ttgcgatgac agcctcgaat acttacggac
     4801 aattactggc ggttggcgca acgtcataca ttattttgca agcattgatc aacgtcaacg
     4861 tgatgaccgg catgttgccg ctgacgggta ttcccttgcc gtttgtgagt tatggcggct
     4921 cgtcattgat ctcggtgatg gcagcggtag gtattctgat tagtgtgtcg cgcggatcac
     4981 gcaaaggaaa aacttacggt gcgtttatgg atcgcagccg gcgggacggg cggtcacgtc
     5041 taccccgcat tagccgtcgt tgagaagatt agggatcaag gatcaggaat cagtgatcag
     5101 ggatcgcgga gtgacccgga gcaatccgaa attttgtgga tcggtggaca cggcggcgtg
     5161 gaagaaaatt tagtggcgcg tgcaaacatt cctttcatcg ctattccggc cggcggcttg
     5221 cacggcgtgg tgatctggtc ggcggtgcgc aacttgatta aattgatcgc aggtacagtg
     5281 aaatccatga aactgatcgg ccaataccaa ccgagcgtca tgtttacgac cggcggcttc
     5341 gtcgctgcgc ccgtggccgt ggcttgctgg ttacgccggg tgcccatcgt cgcatatttg
     5401 cccgatattg aacccggggt ggccgtgaaa tttgtggcgc gcctggccaa gaagatcggt
     5461 gtaacgaccg ccgattcgat cacttacttt cgatcgaaca aagtggtcgt gacgggctac
     5521 cccactcgat cggattttgt gacggccgat cgagcgacgg ccctcgatcg atttgagctg
     5581 gatccgaatc ggaaaacgat tttggttttt ggcggcagca aaggggcgcg atcgatcaac
     5641 cgggcgatcg gatcgatctt accggttcta ttggcgaaat atcaaatcat tcacgtcagc
     5701 ggttcgaccg atgcgttgga ggttcaggcg ctgcgcgatc aactgcccgc cgaggtgcgg
     5761 cggcgctatc atgtctatga gtatctgcat gacatgggtt tggctttggc cgcagccgat
     5821 ttggccgtgt cgcgcgcggg cgcatcgatc ttaggcgagt atccgttgtt cggtttgcca
     5881 tcaatcttga ttccgtatcc gtatgcgtgg cgctatcaga ccgtgaatgc agattatttg
     5941 gcgtcgcagg gtgcggctat tcgtttgatg gatgatgaaa cgttgagcgc gaagttgtcg
     6001 attgagatcg atcgtttgat gaacgacgcg gtaacattgc aagccatgcg cgcggccgcg
     6061 cgttcattag cccagcccga tccggcggag cgtttagccg atgtgattcg ctcggtgagc
     6121 cgggcatgaa cgcactcatc aacgcgaacg atctcatcat tcccgaaatt gccattctgg
     6181 gcttgatctc aatcgcggcg gcggcctggg gttaccggca tggcttggat acggttttga
     6241 tcacggcggt gtttgtggtg tttggtcgcg tgagttccga tattctggcg gtgccggtgg
     6301 cgataattct gaatgtgttg tacggcttct tctcattgat cgccagcgga aaattttcgc
     6361 gcgatgcgtt gaccagcagt atctttggcg gggagaacgg gccgcacttg atcgatacca
     6421 gcaatacggg tgatcaaatt tttgtattga tcagtaccgt gatcttcatc gtagtttgtt
     6481 acatcggcgt gcggttcgcc gtcaaaaaag ccggcggcaa agatccattg atcgaggcgg
     6541 tcttcggcgc gatcggcgga ggagcaatgg cgtatctcac gtttggctac gtgatcaatc
     6601 gggtggtcag tctgccggcc aacattaaga tcacggctac ggat
//
LOCUS       DASRTJ010000340         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_58119,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000340 DASRTJ010000000
VERSION     DASRTJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58119"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VFF70_06950"
     CDS             <1..1456
                     /locus_tag="VFF70_06950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZY44470.1"
                     /translation="SNIVQSTGSLIPASKVQLAFGSSGTVAQVNVALGDHVKKGDVLA
                     SVDATDLQLKVTQAQQSYLIQQLTYSSTVQPDATSIAQAQASYASALASYNAARVSYN
                     TSNASLASQCPGLVTAQTSLDQAQAAYDRLANDHQAKNYLSGDWGPFQSVVNQLTNAQ
                     SAYQLAVANCNIAKTGLTDSSVRSAQAQLQNAKSNLDSLLSPRVETQIQAAAQLEQSR
                     LSYELAKLNLAGATIVAPFDGVVTAVNTQVGLAGSSDAIELADVSQLHVDVLVDETEI
                     ALVQAKQKVELSLDAMAGITLTGQVAWIDPAGTISQGVVNYKVRVNLDATTAPVLLDM
                     TANASIIGETHANVLAVPTTAIQSARGGGNFGGTQGGGNFGGGGNFSGGGNFGGNATG
                     QNGTPAAPGGQTPQRIQGSFVLVVSNGQTQPVQVTVGMTSGDLTEISGNIQEGDEVIV
                     GTVARTPATNNGGGFPGGGFPRGGDNVFVQPPGN"
     gene            1518..2174
                     /locus_tag="VFF70_06955"
     CDS             1518..2174
                     /locus_tag="VFF70_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44471.1"
                     /translation="MIEIEGITKVYQMGETEVRALNGVSLKIEDGEWTAITGPSGSGK
                     STLMAIIGCLDSPTDGRYRLDGIDVAKMSDDKLALVRNQKIGFVFQTFNLLARTSALE
                     NVELPLLYSSKDHRHDRAAAALEAVGLADRMKHHPNELSGGQQQRVAIARALVTEPSI
                     LLADEPTGNLDSKTGAEILSLFRELHSKRGMTVIFVSHDPAIAANAQRVIHIRDGMVV
                     "
     gene            2174..3403
                     /locus_tag="VFF70_06960"
     CDS             2174..3403
                     /locus_tag="VFF70_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44472.1"
                     /translation="MLTQNVRSALRALLANKLRSALTMLGIVIGVGAVVALLSIGTGA
                     QASITSRISGIGANIITVFSGTRNNSAPSGAGGGATAPLTYDEALQLRGLSGVAAVAP
                     QVQSRQPMKNGSKQSTVQVVGVTPDYAIAHPDQLDHGRFVTQSDVSNKSRVTVVGSQL
                     VTDLFGGLDPVGKSVKINGILFQVVGVMKSQGSGGFGFSRDTTSYVPLTTALARLANQ
                     RVGSQQTVSTIEVSAIDQASIDTAIAALTDKMRVLHKIKPGATDDFTVQSQADILAAA
                     SSIATTLTVFLGAIAGISLVVGGIGIMNIMLVSVTERTREIGLRKAVGARKRDILYQF
                     LVETITVSLLGGIIGISIGAAISGLVNASGLISTVVSIESVLLAFGFSAAVGVFFGIY
                     PATRAAGLKPIEALRYE"
     gene            3493..>3770
                     /locus_tag="VFF70_06965"
     CDS             3493..>3770
                     /locus_tag="VFF70_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44473.1"
                     /translation="MENPQIQNEVPPTNGVGVTKRNNRTPLIVGGVVALLVVVAAVVF
                     LVTQSQANQQAATSFQRLGGSAQGLNGQFGGEGEPNDGQGGGQFGVPGG"
BASE COUNT          827 a          965 c         1147 g          831 t
ORIGIN      
        1 gtcgaatatc gttcaatcga cgggcagcct gattccagcc agcaaggtgc agttggcgtt
       61 tggctcgtcc ggcacggtgg cgcaggtcaa cgtcgcttta ggcgatcacg tcaagaaagg
      121 cgatgtcctc gcctcagttg acgcgacgga tctgcagtta aaagtgacgc aggcccagca
      181 atcttatctg atccaacagt tgacttacag ttcaaccgtt cagccggacg cgacctcgat
      241 tgcgcaggcg caggcctcat atgccagcgc gctggcttca tataacgcgg ctcgcgtctc
      301 ttacaatact tcgaatgcca gtctggcttc gcaatgcccc ggcctggtga cagcgcagac
      361 gagtcttgat caagcgcagg cggcttacga tcgattggcg aatgatcatc aagccaagaa
      421 ttatctatcg ggtgattggg gaccgttcca gtctgtggtc aatcaattga cgaatgccca
      481 atcggcttac cagctggcgg tggccaattg caatatcgcc aaaacgggtt tgactgacag
      541 ctcagtgcga tcagctcaag ctcagctgca aaacgccaaa tcgaatctgg atagtttgct
      601 ctcgccgcgg gttgaaactc aaattcaagc ggccgcgcaa ttagagcaat cgcgtctgtc
      661 ttacgaactg gccaaactta acctggcggg cgcgactatt gtggcgccgt tcgacggcgt
      721 ggtcacggcg gttaataccc aggtcggcct ggctggatca agtgacgcga tcgaattggc
      781 tgatgtcagt caattgcatg tcgatgtgct ggtcgatgag accgagatcg cgctggttca
      841 agccaagcaa aaagtcgagc tgtcgctcga tgccatggcg ggtattacct taaccggcca
      901 agtggcgtgg atcgatccgg ctggcacgat cagtcagggc gtggtgaatt acaaagtgcg
      961 agtcaatctc gatgccacaa ctgcgccggt gctgttggac atgacagcga atgccagcat
     1021 catcggtgaa acgcatgcga atgtgctggc cgtgcctacg accgccattc agtcagcaag
     1081 aggtggtgga aattttggag gcactcaagg tggcggcaat ttcggcggtg gcggaaactt
     1141 cagtggcggt ggaaattttg gtggcaatgc aacaggtcag aatggaactc cagcagcacc
     1201 gggcgggcag actccacaac ggattcaagg cagctttgtg ctagtggtgt cgaacggtca
     1261 aacgcagccg gtacaagtta ccgtaggtat gacttccggc gatctgactg aaatctccgg
     1321 caacattcaa gaaggcgatg aggtcatcgt tggcacagtt gcgcgcacgc ccgccacgaa
     1381 taatggcggt ggttttccgg gcggcggctt tccacgcggc ggcgataatg tcttcgtgca
     1441 accgccaggc aattaggaac gcgtgacatg actctgggca tccgcgaaga gatgaacgac
     1501 ttacaagaag gcctgcaatg attgaaatcg aaggcattac caaagtttat caaatgggcg
     1561 agactgaagt gcgcgcgctc aacggtgtct cattgaagat tgaagatggc gagtggactg
     1621 ccatcaccgg cccatcaggc tcaggcaaga gcacgttgat ggcgattatc ggttgtctcg
     1681 attcgcccac cgacggccgc tatcgtctcg atgggatcga tgtcgcgaag atgagcgatg
     1741 acaaattggc actggtgcgg aaccagaaaa tcggtttcgt gtttcagact ttcaatttgc
     1801 tggcgcggac aagtgcgctg gaaaatgtcg aactgccgct gctgtactcc agcaaagacc
     1861 atcggcacga tcgagccgca gcagcgcttg aagccgttgg cctagccgat cggatgaagc
     1921 atcatccgaa tgaattatcg ggcgggcaac aacagcgggt ggcgatcgcg cgcgcgctgg
     1981 tgacggagcc atcgatcttg ctggccgatg aaccgacggg caatctcgat tccaagacgg
     2041 gcgctgaaat cctgtcgctc tttcgtgagc tgcacagcaa gcgcgggatg acggtgatct
     2101 ttgtgtcgca cgatccggcg attgccgcga atgcgcagcg ggtgatccat atccgggacg
     2161 ggatggtggt ctaatgttaa ctcaaaatgt acgcagcgct ttacgcgctt tattggcaaa
     2221 taaattgcgg tcagccttga cgatgctcgg cattgtcatt ggagtgggcg cggtcgtggc
     2281 gctgttgtcg atcgggacgg gcgcgcaggc gtcgatcacg agtcgcatct cgggcatcgg
     2341 cgcgaatatc atcaccgttt tttccggcac gcgcaataat tcggcgccgt ccggcgcggg
     2401 cggcggcgcg acggcgccgt tgacctatga tgaggcgttg caattgcgcg gcttgtccgg
     2461 ggtggccgcc gtcgcgccgc aggttcagtc gcgtcaaccg atgaagaatg gcagcaaaca
     2521 atcgaccgtg caagtggtgg gtgttacgcc cgattatgcg attgcccatc ccgatcaact
     2581 cgatcacggg cgctttgtga cgcagagcga tgtgagtaac aaatcgcgtg tgacggtggt
     2641 cggctcacaa ttggtgactg atcttttcgg cggccttgat ccggtaggta aatcggtcaa
     2701 gatcaacggt attctttttc aagtggtcgg tgtgatgaag tcgcaaggct caggcggttt
     2761 tggcttcagc cgcgatacca cttcatacgt tccgttgact acggcccttg cgcgcttggc
     2821 taaccagcga gtgggtagtc agcaaacggt ctcaacgatc gaagtctcgg cgatcgatca
     2881 agccagcatc gacacggcga ttgcggccct cacggacaaa atgcgcgtgc tgcacaagat
     2941 caagccgggc gcaaccgatg acttcactgt tcagtctcaa gccgacattt tagcggcggc
     3001 cagttcgatc gcgacgacgt tgaccgtttt cttgggagcc atcgccggta tttcgttggt
     3061 ggtcggcggt attggcatta tgaatatcat gctggtctcc gtaacggagc gcacgcgcga
     3121 gatcggtctg cgcaaggcgg tcggcgcgcg caagcgtgac atcttgtatc aatttttggt
     3181 ggaaacgatt accgtttcat tgctcggcgg aatcattggc atctcgatcg gggcggcgat
     3241 ctccggcctg gtgaatgcca gcggcttgat cagcacggtg gtctcgatcg aatcggtctt
     3301 gttggctttc ggtttctcgg cagcggtcgg cgtgttcttt ggaatctatc cggccactcg
     3361 agcagccggg ctgaaaccga tcgaagcgtt gcggtatgaa taatctagaa accttgcgaa
     3421 gggtttagtg cggatcggct attctccaat ccaacatgcg caaggttaat tccttcaagg
     3481 gagtaaacag aaatggaaaa tcctcaaata caaaatgaag tacccccgac aaatggtgtg
     3541 ggtgtgacta agagaaataa tcgcacgccc ttgatcgtcg gcggcgtagt ggccttgctc
     3601 gtcgtagtag cggcggtcgt cttcttggtc actcagtcgc aagcgaatca gcaagctgcg
     3661 acatcttttc aacgcttagg tggcagtgct caaggcttga atggtcaatt cggcggtgag
     3721 ggtgaaccca atgatgggca aggcggcgga caattcggtg tgcccggtgg
//
LOCUS       DASRTJ010000341         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_95321,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000341 DASRTJ010000000
VERSION     DASRTJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95321"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFF70_06970"
     CDS             complement(<1..337)
                     /locus_tag="VFF70_06970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZY44474.1"
                     /translation="MPLTHVAKFKVRYTECDQYGHVNNANYLRYMQEAAFGASAQAGY
                     DFARYDEIGQYWLVRETDIEYLKPLRYGDEFEIKTWVVDFRRVRSRRAYEFRLSGSDD
                     LITRASSDWV"
     gene            complement(358..1530)
                     /locus_tag="VFF70_06975"
     CDS             complement(358..1530)
                     /locus_tag="VFF70_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44475.1"
                     /translation="MRLDRLKLHLNSPWQPCLVAMIANLALVLTRLAVRGNDPSIFVT
                     AGDFFVDRQSAPASLSILNDSFGYDGQFYYRLALDPLTSEHTAFGITLDAPEVRQQRI
                     TYPIVVAILSFGRADLVPIMLIAVNLLALGAIGYLGGLWVKSIGQSALWGVVFPLYTG
                     FQFSLARDLTEILAIGLLLTSLYLARLARSRAATGLLVLAVLTRETTVAVALAAAMIY
                     FFGLITKKKAMVEFKWYYFAIPIAVYAAWQLTLYSRWGALPITVSSQFNVGRPFIGLV
                     QFGLKGLGVGTPFNLRGFGELGLVLSMFFLPALAWRTSTARPSEKLAWLLLALLAVNF
                     ASGVTDEDISLLRVLADLFLFSAIVVFNSPSKIRWLFGGFSVVMWIVSFQYAVNLY"
     gene            complement(1674..1823)
                     /locus_tag="VFF70_06980"
     CDS             complement(1674..1823)
                     /locus_tag="VFF70_06980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZY44476.1"
                     /translation="MPLTHIGKFKVRYTECDQNGHVNNANYLRYMQEAAFDASTSAAN
                     NFDRR"
     gene            complement(1963..2205)
                     /locus_tag="VFF70_06985"
     CDS             complement(1963..2205)
                     /locus_tag="VFF70_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44477.1"
                     /translation="MTVTRDLTEIGAIVFLLTSLFLLRSGKDRWATLLLTLALLTRES
                     TVLFALACAVLYLIQSVRRKSIRLSFKWYYFGVIVI"
     gene            complement(2888..>5038)
                     /locus_tag="VFF70_06990"
     CDS             complement(2888..>5038)
                     /locus_tag="VFF70_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZY44478.1"
                     /translation="CHSQWHQRRSEAKGAAMNESEKEKYLEEYHQDKEKGVPFFPNII
                     FKDAVVSFLVFLILIALAYFVGAPLEARANPGDASYTPRPEWYFLFLFQLLKYFPGNL
                     EVIGVVLLPTLVIVLLFLLPFIDRSPRRHFLNRPIITGVTVLGLVAIAFLTIQSVRET
                     PPPAAAAGGDTTAGLYSKNCAACHGSSIVVPAGTNLHTLIAQGKHADMPAWSADLTTD
                     QIDALAGFISSPGGSQLFTQYCGDCHKTPESIVGDPIKLRDVLDQGPSYPAHAAVNVP
                     QWTSVISPEQRIALLNFLVAPDGQRLFAVNCSGCHGSSVAFSGETTALRDLISKGGQH
                     LEMPAWQAKLSPDQLDTLADYVLDPVAVPDGKPIFDKFCAVCHGQRVPTAIDYTQARD
                     AIANGGSHKTMPVWGTILTPEQMDALVKYTLDVAKGTPIQVGQNLFARNCTQCHGSFG
                     EGGLNPTRPGDIIAPISTAEFLKTRDDATLRAIVAQGQPNFGMSPFGTSFGGPLSDEE
                     VDSIVAYIRSWQANPPVELPPDIAVARVSLSSSGIFSGLCAQCHGLKGEGGIGPSFRS
                     SVFQTNNTDQNIFDTIDKGHPATAMIAWGEILDPGQIEQLVGYIRLLGRAAPEPTGAT
                     RTFAADVKPIFEAQCSSCHGSKGQWDAASYDSVMETGQHAPTVIAGDFKNSLLMQKIL
                     GTQTEGDLMPPTDRLSDVQIQIILDWIASGALEK"
BASE COUNT         1275 a         1342 c         1356 g         1065 t
ORIGIN      
        1 acacccaatc gctcgatgcg cgcgtgatca gatcgtccga tccgctgagt cggaattcgt
       61 acgcgcggcg cgatcgcacg cgtcgaaaat cgacgaccca ggttttgatc tcgaactcat
      121 cgccgtagcg cagcggcttg agatattcga tatcggtttc acgcactaac cagtattgcc
      181 cgatttcatc gtaccgcgcg aaatcatacc cggcctgcgc tgatgcgccg aacgcggctt
      241 cttgcatata gcgcaagtaa ttggcgttgt tgacatggcc gtattgatcg cactcggtgt
      301 agcggacttt gaatttggca acgtgcgtta gaggcatagc ccagcctgtg gtgatcctta
      361 atagaggtta actgcgtact gaaacgaaac gatccacatc actactgaaa aaccaccaaa
      421 caaccaccta attttagagg gtgaattgaa tacgacgatc gcgctgaaaa gaaacaaatc
      481 ggctagaacc cgcaaaagac tgatatcttc gtcggtgacg cctgaagcga aatttacggc
      541 caacagcgcc agcaatagcc aggccagttt ctcagatgga cgtgcagtgg acgtgcgcca
      601 cgctaacgcc ggtaagaaaa acatacttag gaccaggccc agctcgccga agccgcgcaa
      661 gttaaagggt gtcccgactc cgaggccctt caatccgaat tgcactaaac caatgaacgg
      721 gcggcccaca ttaaattggc tggagacggt gatcggcagt gcgccccagc gcgaatataa
      781 cgtcagctgc caggccgcat agaccgcgat cgggatcgca aaataatacc atttgaattc
      841 taccatcgct ttttttttcg tgataagacc gaaaaagtaa atcatcgccg ccgctagtgc
      901 aactgccacc gtcgtctcgc gagtcaacac ggccaagacc aacagcccgg tggccgctcg
      961 agatcgagct aatcgcgcca gatacaaact ggtcaatagc aacccgatgg ctaggatctc
     1021 agtgagatcg cgcgccagag aaaattgaaa gccggtatat aaggggaaga ctactcccca
     1081 cagtgcagac tgaccgatcg atttgaccca caggccgccg agataaccga ttgcccccaa
     1141 agccaacagg ttgacggcaa tcaacatgat cggcacaagg tccgcgcgac cgaaggataa
     1201 tatggcgacg acaatgggat acgtgatgcg ttgttgacgg acctccgggg catccaatgt
     1261 gatgccaaag gccgtgtgct cggacgtcaa cggatcgagt gccaagcggt aataaaattg
     1321 gccgtcataa ccgaacgagt cattcaagat cgatagggaa gccggcgccg attggcgatc
     1381 tacaaaaaaa tcacccgctg tcacaaagat ggatggatcg ttgccacgta ccgctaatcg
     1441 ggtcaggaca agtgccagat tggcgatcat cgcgacgaga cacggctgcc agggagaatt
     1501 aagatgcaac ttcaaccgat ccagtctcat gggttgcgcc tagaataaaa gggggcgagc
     1561 tcattgctcg cccctgtcgc taaagaaaaa tttacagctt cggctggctg attgctgtcc
     1621 atcaagctgt tcaggcggct acctaagtaa gtggccacat agtattacga ttttcaccgc
     1681 ctgtcaaaat tgttggccgc cgaggtggac gcatcgaacg cggcttcctg catgtagcgc
     1741 aagtaattgg cgttgttgac gtggccgttt tgatcgcatt cggtgtaacg gactttgaat
     1801 ttcccaatgt gggttaaggg catgcgggtt tcctgcatca ataattgtaa tgactggcta
     1861 aaagccagac tgcgagcacg tatatgaaga gtaacttctt agacctctgc ggcgagccta
     1921 acaagacaac gcctgccagc gtcgataagt ccgccggcac tctcaaataa ctatcacgcc
     1981 aaagtaatac catttgaagc ttagcctgat cgattttcgc cgtacgcttt gaatcagata
     2041 gagcactgca caagccaacg cgaacagcac tgtcgactcg cgcgtaagta aggcgagtgt
     2101 caacaacagt gtcgcccaac gatccttgcc cgaccgcagc aaaaataaac tggtcagcaa
     2161 aaagactatc gcgccgattt cagtgagatc gcgggtgacg gtcataaggt aatctgctta
     2221 gaatgggaat aataatcccc acagcgcatg cagcctgatc gatttggccc agactccgcc
     2281 tacctagccc aacacgccca aggctaaaaa atttacggcg atcaatagcc atggcaccca
     2341 ttgggtttga cccgctgctg acgataagcc ggaagatcga agtgaatgcc ataatcgacg
     2401 ggctgcgtat taaaaggatc tagcgccagc cggtagtaga attggccgtc gtagccggat
     2461 ttaccctgac gaattaataa cgaggcgggg gcttggctcg gatcgataaa aacgtcaccg
     2521 gcgataatga aggcggacgg atcgccctga gacctgatca attggaatag cagaaaaatg
     2581 aaatatgaag ctaacgtaat cgcgcaagtc agtcagagtg aatcagtccg ctgctttaat
     2641 ttcgccagca tctgtggtcc tttacgaata cttcgggcct aagcgccaca tcctaacact
     2701 catgcaagcg tgcgtcaacg cactcgcaat agaaaaacta aataaaatca ggacagtgca
     2761 aatagtcaaa aacggaactg gcggcgcacg ctaaaacaat cggggcaatg cggattgatc
     2821 tgctagatcg atcctgcatt gcccctgccc gatccttcct ccgaatcagg ttaaaacatc
     2881 gatcgattta tttttccagt gcgccggatg cgatccaatc caagatgatt tgaatctgga
     2941 catcggatag tcgatccgtg ggtggcatca agtcgccttc agtttgagtg cccagtatct
     3001 tctgcatcag cagactgttc ttgaagtcgc ccgcaatgac agtcggcgcg tgttgaccgg
     3061 tctccatcac ggaatcgtag ctcgctgcgt cccattggcc tttgctgccg tggcagctgc
     3121 tgcattgagc ctcaaagatc ggtttcacat cggcggcgaa ggttcgagtc gcaccagtcg
     3181 gctcaggcgc agcgcggccg agcagtcgga tataacccac cagctgttcg atctgacctg
     3241 ggtcgagaat ttctccccag gcgatcatcg ccgttgcggg atggcctttg tcgatcgtgt
     3301 caaagatgtt ctgatcggtg ttgttcgttt ggaacacact actgcggaag gacgggccaa
     3361 taccgccttc acctttaagg ccatggcact gcgcgcacag gccggagaaa ataccggatg
     3421 aactgagcga cacgcgcgcc acggcgatat caggcggcag ttcgaccggc ggattcgcct
     3481 gccacgatcg gatgtaggcc acgatggaat ctacttcttc atcgctgagc ggtccaccga
     3541 aagacgtgcc gaatggcgac atgccaaagt tgggctgtcc ctgcgcaacg atcgctcgca
     3601 gtgtggcatc atcgcgcgtc ttgagaaatt cggcggtact gatcggggca atgatatcgc
     3661 cgggccgagt aggattaagt ccaccttcgc cgaagctgcc atggcattgg gtacagttgc
     3721 gcgcaaatag attttgaccg acttgaatgg gtgtgccttt ggcgacatcc agtgtgtatt
     3781 ttactaaggc gtccatctgc tctggtgtaa ggatggtgcc ccagaccggc atggttttgt
     3841 gtgagccgcc attggcgatg gcgtcgcgcg cttgagtgta atcaatggct gtcggcacgc
     3901 gctgaccgtg acatacggcg cagaatttat cgaatatggg tttaccgtca ggcacggcta
     3961 ccggatcgag cacatagtca gcgagcgtat ccagctgatc aggcgatagt ttagcctgcc
     4021 aggccggcat ttcaagatgc tgcccgccct tactgatcag atcgcgcagg gcagtggtct
     4081 cgcctgaaaa tgccacgctc gatccatggc agccggagca attgacggcg aacaagcgct
     4141 gtccgtccgg cgcgaccaga aaattgagca acgcgatgcg ctgttccggg ctgatgacgc
     4201 tggtccattg cgggacattc accgctgcat gcgccggata actcggtcct tgatccaata
     4261 catctctgag cttgatcgga tcaccgacaa tcgactccgg cgttttgtgg caatcaccgc
     4321 agtactgtgt gaataactgg ctgccacccg gcgagctgat gaagccagcc aaggcatcga
     4381 tctgatcggt cgtcagatca gcgctccagg ccggcatatc ggcatgtttg ccttgagcga
     4441 tgagagtatg cagattcgtc cccgctggca ccacaatgga tgatccatga caggcggcac
     4501 agttcttgga atacagcccg gcagtggtat caccgcccgc tgcggcggcg ggcggcggtg
     4561 tttctcgaac ggattgaatc gttaagaaag cgatggccac tagccccaaa acagtcacac
     4621 cggtaatgat tgggcgatta aggaagtgcc ggcgcggcga tcgatcgatg aacggcaaca
     4681 ggaatagcag tacgatcacc agagtgggga gcaggacaac gccgatgact tccaggttgc
     4741 ccgggaaata tttcaacagc tggaacagga acaagaaata ccattcaggc cgcggtgtgt
     4801 agctggcatc acccggattg gcccgcgctt cgagcggcgc gccgacgaag taggccaatg
     4861 cgatcaagat gagaaacacc aaaaaggata caactgcgtc cttgaatata atgttcggaa
     4921 aaaacggaac gcccttttct ttatcttgat gatactcttc tagatatttt tctttctcac
     4981 tctcgttcat ggccgctcct ttcgcttcgg aacggcgctg atgccattgc gaatgaca
//
LOCUS       DASRTJ010000342         1806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_20312,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000342 DASRTJ010000000
VERSION     DASRTJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20312"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFF70_06995"
     CDS             <1..656
                     /locus_tag="VFF70_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY44479.1"
                     /translation="FTATLIAVCDQLKLDRPAVVGHSMGGTIALDLALNLETSAVFVA
                     APIITGELTFSVHLLLTSPAARRFLRWLRRQRFFSALGSMKPIAVPNLTRDPSRKRRQ
                     QDLQRTTLNAAVGSLRTVVGAHLEDHLEEITAPTLILVGGRDSTVAPSQGKLAALRIP
                     NARLVEWPAVGHAMVDDRPDEFDQLLINHLKSTTQAKAEPTAAAARSLRGDLVAASS"
     gene            659..1249
                     /gene="thpR"
                     /locus_tag="VFF70_07000"
     CDS             659..1249
                     /gene="thpR"
                     /locus_tag="VFF70_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZY44480.1"
                     /translation="MDAIRTFIAIELPQAILDQLSEIQARLKSAVPGHAMRWVKPDSI
                     HLTLKFLGQTPDDQIGLITSSMKAGLAGHPAFTLEISGAGCYPTVQRPRVIWIGVHED
                     QNAKHLSAVQHAVESSIAPLGYPTERRDFSPHLTLGRVSHEVQPADLKKIGAAIGANA
                     VGLLGRFEVIQVALIRSDLKPHGAVYTILARAPLKS"
     gene            1262..>1806
                     /locus_tag="VFF70_07005"
     CDS             1262..>1806
                     /locus_tag="VFF70_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZY44481.1"
                     /translation="MSFQPMFIDRVDAGQKLAEALIDLRDRSDSIVVLAIPRGGVIVG
                     REVARSFHASFDVILTHKLGAPDNPELAIGAVAEDGRTIILDQTLLNYLQISPEYLQA
                     EIDRQKVEMQRRAAIYRRGRERLAVTHKIVIVVDDGVATGSTLIAALRSIRTAGSQEV
                     IAAVPVGPNQVVRQLRHEADRV"
BASE COUNT          366 a          508 c          512 g          420 t
ORIGIN      
        1 attttacggc cacgctgatc gccgtgtgcg atcaacttaa actcgatcga ccggctgtgg
       61 tcgggcattc gatgggcggc acgattgcgt tggatcttgc cttaaatctg gagacgagcg
      121 cagtcttcgt tgccgcgccg atcattaccg gcgaactcac tttctcggtc catctgctgc
      181 tcacttcgcc cgccgcgcgc cgttttttgc ggtggctgcg ccgccaaaga tttttctcgg
      241 cccttggttc aatgaaaccg atcgctgtgc cgaacttgac gcgcgatccg agccgtaaac
      301 gtcgccagca agatttgcag cgcacgactt tgaatgccgc tgtcggcagc ctgcgcaccg
      361 tggtcggcgc gcaccttgaa gatcatctgg aagaaatcac agcgccgacg ctgattttag
      421 tcggcgggcg cgattctacc gtcgcgcctt cgcaaggcaa acttgccgcg ctgcgaatcc
      481 ccaatgcccg cctggtggag tggcccgccg tcggtcatgc catggtagac gatcgaccgg
      541 atgaatttga tcaattactg atcaatcatt tgaaatctac cacgcaggcg aaagccgagc
      601 ccactgctgc ggccgctcga tcgctgcgcg gcgatctcgt cgctgcctcg agctaaacat
      661 ggacgccatt cggaccttca tcgccattga attgcctcag gcgatccttg atcaattgag
      721 tgagattcaa gcgcgcctga aatccgccgt acccggccat gcgatgcgct gggtcaaacc
      781 ggacagtatt catttgacgc tgaagttcct cggtcaaacc ccggatgatc aaattgggtt
      841 gattacttca tcgatgaaag caggcctcgc cgggcatccg gcatttacat tagaaatttc
      901 cggcgcgggt tgttatccta ccgtccaacg gccgcgtgtg atctggatcg gcgtgcacga
      961 agatcaaaac gccaaacatc tgagcgctgt tcagcatgcg gtcgagtcat cgattgcgcc
     1021 actgggctat cccaccgagc gccgagattt ttcgccgcac ttgactctgg gacgtgtgtc
     1081 ccacgaggta cagcccgctg atcttaagaa gatcggtgcg gctattggtg cgaacgcggt
     1141 cggattattg ggccgcttcg aagtgatcca agtcgctttg attcgcagcg atctaaaacc
     1201 acatggcgca gtgtacacta tccttgcgcg cgcaccattg aaatcgtagg gacaaacccc
     1261 attgtccttc caacctatgt ttatcgatcg agttgatgcg gggcagaaac tagccgaagc
     1321 cttgatcgat ctgcgcgatc gatcggattc gattgttgtg ttggcgattc cgcgcggcgg
     1381 tgtgattgtc ggccgtgaag tagctcgatc gttccacgcg tccttcgatg ttatcctcac
     1441 ccataagctt ggcgcacccg ataatcccga attggctatc ggcgcggtgg ccgaagatgg
     1501 tcgaacgatc attttagatc aaaccttgct gaattatctg cagatttcgc cagagtatct
     1561 tcaggcggag atcgatcgtc agaaggttga gatgcagcgc cgtgcggcga tttatcgccg
     1621 agggcgtgaa cgcttagcgg tgacgcataa gatcgtgatt gttgtggatg acggcgtggc
     1681 aaccggctcg acgctgatcg ccgcattgcg ttcgattcga acagcaggat cgcaggaggt
     1741 gatcgccgcc gtgccggtag ggccgaatca agtcgttcgc caactgcggc atgaagctga
     1801 tcgtgt
//
LOCUS       DASRTJ010000343         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_20342,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000343 DASRTJ010000000
VERSION     DASRTJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20342"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1356
                     /locus_tag="VFF70_07010"
     CDS             43..1356
                     /locus_tag="VFF70_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44482.1"
                     /translation="MTTSPIERPRLSHFRQSMLSLYWFATSVQWTAILITTLPNQAYV
                     MGGDRVKGQTLGTILLIGAFVSMFVAPFFGALSDRVITRFGRRKPWIVIGTLMNNVGL
                     LGLAYFPRSNDMSSLPLFILAFMWVEFWNNVATAPYSALIPDIVPIEQRGSASGWYGL
                     MNMLGSFVGGVAGLIFTQNGVTDVVSIYYFIGAINLIGLAGTIVFVKELPVSIVLPAI
                     KFKKFMHSLIEPLRDHDFRWVFGTRFLMIMGAFTVQEFLQYYMRDVVKDFTLFGVRVA
                     ENAESAVSFFIIALLIGAVASSLAAGILSDKLGRKKIVYLSSILQAIVPFVLIFFSPF
                     PLVVGLGIIFGLGYGAYASVDWAMVSDVLPSDADHAKDMGVWHVADTFPQVIATPIAG
                     LLLDRFQVVGPTLNQPTLGYTVIFSLAVVYFVLGTVFVRKIRKVK"
     gene            1453..>2374
                     /locus_tag="VFF70_07015"
     CDS             1453..>2374
                     /locus_tag="VFF70_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY44483.1"
                     /translation="MATYDVAVIGGGPAGYVAAIRASQLGLKAAIVEREYLGGVCLSW
                     GCIPSKALLRNAEIANLLTRGKEFGFAFENLKLDYSVAQKRSRQISDRLIKGVQFLMK
                     KNKIDVFQGTGTLKDKTHIEVKSVPNAPGVNGQTFDGVIESKNIVIATGARARSIPGV
                     TIDGQAIITYRQALEITEPPKSILIIGAGPIGVEIGYVFRSYGTDVTILEMLPNVVPL
                     EDEEVSAELAKQLTKQGIKLMLGAKVESVAKSGGGVEVKAGGQSIMADKALIAIGFTP
                     NSENLGLEAVGVKIDRGAIVVDSHFRTNVPN"
BASE COUNT          488 a          576 c          716 g          594 t
ORIGIN      
        1 tttagggggc gatcaatcga tcgcccctct acgtgtattc atatgactac ctcaccgatc
       61 gaacgtccac gccttagtca ctttcggcaa tcgatgctgt cgctgtactg gtttgcgaca
      121 agcgtccaat ggacagcgat cttgatcacg acgctgccga atcaagcgta cgtcatgggc
      181 ggcgatcgcg tgaaaggcca gacgcttggc acgatcttgt tgatcggcgc gtttgtgtcg
      241 atgttcgtcg cgcctttttt cggtgcgctc agcgatcggg tcatcacgcg cttcgggcgc
      301 cgcaaaccgt ggattgtgat cggcacgttg atgaataacg tgggcttgtt gggtctcgcc
      361 tactttccac gctcgaacga tatgtcatcg ctgcccttgt ttatcctcgc tttcatgtgg
      421 gtcgagtttt ggaacaacgt ggcaacagcg ccgtactcgg ccttgattcc tgacatcgtg
      481 ccgatcgaac aacgcggcag tgcttccggt tggtatggct tgatgaatat gctcggcagt
      541 ttcgtcggcg gtgtggccgg tttgatcttt acgcaaaacg gtgtgaccga tgtggtgtcg
      601 atctattact tcatcggcgc gatcaacttg atcgggctgg cgggcacgat cgtgtttgtg
      661 aaggaactac cggtctcgat cgtgctgccc gcgatcaagt tcaagaaatt tatgcacagt
      721 ttgatcgagc cgctgcgcga tcacgatttt cgctgggtgt tcggcacgcg cttcttgatg
      781 atcatgggcg cgttcactgt tcaagaattc ttgcagtatt acatgcgcga tgtcgtcaaa
      841 gacttcacac tattcggcgt tcgcgtcgcg gagaatgccg agtcggcggt atcgttcttc
      901 atcattgcgc tgctgattgg cgccgtcgcg agttcattgg ccgccggcat tctatcggat
      961 aagctaggtc gcaagaagat cgtgtatcta tcgagcatcc tgcaagcgat cgtgcccttc
     1021 gtcttgatct tcttcagtcc gtttccgctt gttgtgggac tgggtattat ttttggattg
     1081 ggttatggcg cgtatgcgtc ggtcgattgg gcgatggtga gcgatgtgct gccgagcgat
     1141 gccgatcacg cgaaggacat gggcgtgtgg catgtggccg atacttttcc gcaggtcatc
     1201 gcgaccccga tcgcgggtct gctgctcgat cggttccagg ttgtcgggcc aacgctgaat
     1261 cagccgacgt tgggctacac cgtcattttt tcgttagctg ttgtatactt tgtgttgggc
     1321 acggtgttcg tacgcaagat tcgcaaagtg aaataaatag cgaactacag gatagaaggg
     1381 cacagagaat ttaagaagta ggggcgagcg gctgctcgcc ctaatcacga taatttctta
     1441 tgaggtgaat gaatggcaac ctatgatgta gcagttattg gcggcgggcc ggcgggttat
     1501 gtggctgcga tccgcgcctc acagttgggt ttgaaggccg cgatcgtcga gcgggaatat
     1561 ttgggcggtg tgtgtttgag ttggggctgt attccatcga aggcgctgct gcgcaatgcc
     1621 gagatcgcaa atttgttgac gcgcggcaaa gagttcggtt tcgcgtttga aaatctcaaa
     1681 ctcgattatt cagtcgcgca aaaacgcagc cgccaaatca gcgatcgatt gatcaagggc
     1741 gtgcagttct tgatgaagaa gaacaagatc gatgtgttcc aaggcacggg cacgctgaaa
     1801 gacaagacgc acatcgaagt gaagtctgtg ccgaatgcac ctggtgtgaa cggccagacg
     1861 ttcgatggcg tgatcgaatc taagaacatc gtgatcgcga ccggtgcgcg cgcccgatcg
     1921 atccccggcg tcacgattga tggtcaggcg atcatcacgt accgacaggc gttggagatc
     1981 accgagccgc cgaaatcgat cctgatcatt ggtgcaggcc cgatcggcgt tgagattggc
     2041 tatgtattcc gatcgtatgg cacggatgtg acgatcctcg agatgctgcc caacgtcgtg
     2101 ccgctggaag acgaagaggt cagcgccgaa ctcgccaagc agttaacgaa gcaaggcatc
     2161 aagttgatgc ttggtgcgaa agtcgaatcg gtcgccaaat ccggcggtgg tgttgaagtc
     2221 aaagcgggcg gccaatcgat catggccgat aaggcgctaa tcgcgatcgg gtttacgccg
     2281 aattctgaaa acttgggact ggaagcagtc ggcgtgaaga tcgatcgggg cgcgattgtt
     2341 gttgatagcc atttccgtac gaacgtgccg aaca
//
LOCUS       DASRTJ010000344         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_122130,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000344 DASRTJ010000000
VERSION     DASRTJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122130"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFF70_07020"
     CDS             complement(<1..372)
                     /locus_tag="VFF70_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HZY44484.1"
                     /translation="MGFDFDTYVVLDVPMPVAAQVMAVRERHRDEFRASLPVEITVVG
                     SSGIGVFDPTQDSAVAFATLDYMAAEIVPIHASFGKVIRFPDTDIFVLTLNDERPFRA
                     LHERIAKSGLRFLPNSFPYKPH"
     gene            complement(418..>1783)
                     /gene="proS"
                     /locus_tag="VFF70_07025"
     CDS             complement(418..>1783)
                     /gene="proS"
                     /locus_tag="VFF70_07025"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018730053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZY44485.1"
                     /translation="HIQTKFRDDPRPRAGLIRVREFTMKDSYSLDVDDAGLDKQYRAH
                     YQAYFNIFNRCALPAIAVGSDVGMMGGSLAHEYMYLAAIGEDTLVLCRNCGYAANRQI
                     ARFRKAQNPIEQLRPIEKVPTPECKTIDALATVLNVPKSKTAKAVFMIAGHEAEGQTR
                     EEFIFAVVRGDMDANETKLNNAVKADWLRPATDEEIKAIGATPGYASPIGLKNVTVVV
                     DDAVPASTNLVAGANEEGYHLLNTNTPRDYAVSIVADIAAASDGDACIDCGHELYTSR
                     GVEVGNIFKLGTRYSAAVGATFLAADGTQQPIVMGSYGIGSGRLLACIAEEHHDDKGL
                     IWPITVAPYQVHLVSLGNDAAINDKAEWAYRALIDAGIDVLFDDRAESPGVKFADADL
                     IGLPIRLTVSTKSIGKGGFEMKRRDRKELEIVSESDLVVHVRALIESMYEEIANRVVE
                     VPFK"
BASE COUNT          407 a          505 c          461 g          410 t
ORIGIN      
        1 atgcggctta tagggaaaag agtttggtag aaaacgcaaa cccgactttg ctatacgttc
       61 gtgcagagcg cgaaatggcc gctcgtcatt gagagtgaga acgaagatgt cagtgtccgg
      121 gaagcgaatt actttcccaa aagacgcgtg aatagggaca atttcagcgg ccatataatc
      181 aagtgtggca aaagcaactg ctgaatcttg tgtaggatcg aatacgccga ttccgcttga
      241 tcctacgacg gtaatctcga ctggaagcga tgcacggaat tcatcgcgat ggcgttctcg
      301 aacagccata acctgagctg caacaggcat aggaacatct agaactacgt atgtatcgaa
      361 atcaaatccc atttcacttc ttccatgtct gaggcaggtt acgaaatcaa tccactctta
      421 cttaaacgga acttcgacca ctcgattcgc aatctcttca tacatcgact cgatcaacgc
      481 gcggacatgc acgacgagat cagactcact cacgatctcc aattctttgc gatcgcggcg
      541 cttcatttca aagccgcctt tgccgatcga tttggtgctg acggtcaagc gaatgggcag
      601 accgatcaaa tccgcatcgg cgaatttcac gcccggactt tcagcgcgat cgtcgaacag
      661 cacatcgatg cccgcatcga tcaaggcgcg atacgcccat tcggccttgt cgttgatcgc
      721 cgcatcattg cccagcgaaa ctaaatggac ttgatacggt gcaacggtga tcggccagat
      781 caaaccttta tcatcgtgat gctcttcggc gatgcaggct aacaagcggc cagagccgat
      841 gccatacgag cccatcacga tcggttgctg agttccatcg gcggcgagga acgttgcgcc
      901 gaccgcggct gaatagcgcg tacccaattt gaaaatgttg ccgacttcga cgccgcgcga
      961 tgtatacagt tcatgtccgc aatcaatgca cgcatcacca tcactcgccg ccgcaatgtc
     1021 ggcgacgatc gatacggcgt aatcgcgcgg cgtgttggtg ttgagcaggt gataaccctc
     1081 ttcgttcgcg cccgcgacta agttagtcga tgcaggcacg gcatcgtcaa caacaactgt
     1141 tacattcttc aaaccgatcg gcgatgcata acccggcgtc gcgccgatgg ctttgatctc
     1201 ctcatccgtc gcgggccgca gccaatcggc cttgaccgcg ttgttcaact tcgtttcgtt
     1261 ggcatccata tcgccgcgca ccacggcgaa aataaactct tcacgcgttt gtccttctgc
     1321 ttcatgtccc gcgatcatga acacagcctt cgccgttttg ctcttgggca cattcaacac
     1381 agttgccaac gcgtcgatcg tcttgcactc gggcgtcggc actttctcga tcggccggag
     1441 ctgctcgatc gggttctgcg cttttctaaa gcgcgcaatt tgtcgattgg ccgcatagcc
     1501 gcagttgcgg cacaacacca gtgtgtcttc accaatcgct gcgaggtaca tgtattcatg
     1561 cgcgagcgag ccgcccatca tgcccacgtc cgatccgacg gcgatggcgg gcagggcgca
     1621 gcgattgaag atgttgaagt aagcttgata gtgcgcgcga tattgtttgt ctaagcccgc
     1681 gtcgtccaca tcgaggctat atgaatcttt catcgtgaac tcgcgcacgc ggatcagccc
     1741 ggcgcgtggt cgcgggtcgt cgcggaactt ggtttggatg tga
//
LOCUS       DASRTJ010000345         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_95694,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000345 DASRTJ010000000
VERSION     DASRTJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95694"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFF70_07030"
     CDS             complement(<1..542)
                     /locus_tag="VFF70_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZY44486.1"
                     /translation="MYGFTGKILHVDLTRGTLTAETPEEKFYRTYWGGSAMGLYYLLK
                     NTPPKADPLGPQNTLCFFISASTGVAISGQSRCTATAKSPLTDLVGDAQAGGFWPAEL
                     KFAGFDGIVITGKAVKPVYLWLHDGQAELKDATHLWGKGFTTGEAEKHIRTELGDAKI
                     EVAQIGPSGEKQINLASIIN"
     gene            complement(665..1474)
                     /locus_tag="VFF70_07035"
     CDS             complement(665..1474)
                     /locus_tag="VFF70_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44487.1"
                     /translation="MLAKLAELWRYRELVYNLTVRDLKVRYKNSVLGIAWSLLNPLLM
                     MLVLTLVYTVMLGQSNRHDYPAFILSGLLPWNFFSASIMGGTRSIVDNSHLIKKVYFP
                     REVLPISLTLSSLINFLMALPVLIVLSLILGVQLTGWIVFLPLVILVQMIFSMGVSLF
                     LAATNVFYRDVQQIMEVAILAWFFLTPIIWDVSLLPASKEIFGLTVPIQRLTYILNPM
                     ASIVATYRDILYYGRPIGWDFFLRTILTAIIMLFIGYAVFHRLQKRFAEEV"
     gene            complement(1455..>2268)
                     /locus_tag="VFF70_07040"
     CDS             complement(1455..>2268)
                     /locus_tag="VFF70_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY44488.1"
                     /translation="LENAIDRMPRAILTSSQHSAKVLLNEFNLQPDRVIPLPDCVDTE
                     FFRPRSAADQADIDSLKEQLGIPLDRSVVIYLGLLAHYQGTDHIIQAARKVIEAQPNA
                     HFLIMGYPGHLGYQVHANQLGLGDHMTFWGRLPYEQAARYLRLGDVALAPKLSLTEGA
                     GKILNYMACALPTIAFDMPVAREFMADRGTYADRGSADALAGCIIDLLQKPDRRSTIG
                     QALRQRAIDRFSWDEAGDQILSVYDAIRLPRSADRAAALSKIREGKHAGETR"
BASE COUNT          565 a          629 c          594 g          480 t
ORIGIN      
        1 atgttgatga tcgaagccag gttgatctgt ttttcgcccg atgggccgat ctgcgcaacc
       61 tcgatcttgg catcgcctaa ttcggttctg atgtgtttct cggcctcgcc agtagtgaaa
      121 cctttgcccc acaaatgtgt cgcgtctttg agttcagctt gtccatcatg cagccacaaa
      181 tagacgggct taacggcttt tccggtgatc acaatcccat cgaatccggc gaactttaat
      241 tcagcgggcc agaaaccgcc ggcctgcgca tcgccgacaa gatcggtcaa cggacttttc
      301 gccgtggccg tgcagcggct ttgcccggag atcgccacgc cggtcgaggc gctgatgaag
      361 aagcacaatg tattctgcgg ccccagcgga tcggctttgg gcggcgtgtt cttcaataaa
      421 taatacagac ccatggcgct gccgccccaa taggtgcggt agaatttttc ttcaggcgtt
      481 tcggcggtga gggtaccgcg ggtgagatcg acgtgcagaa tcttgccggt aaatccgtac
      541 atcgtatcct cctgatttct ataagttcag attatttggc tgggcaaaag tataacccaa
      601 ggtgatgaag ttgggcaagt tgcaggttgc acgttgcaag ttactcctcg cgccttgccc
      661 ctcgctacac ttcttccgca aatcttttct gcaatcgatg aaacacggca taaccgatga
      721 acaacatgat gatcgccgtc aggatcgtcc gcaggaaaaa gtcccagccg atcggacggc
      781 cataatacag aatgtcgcgg tacgtcgcca cgatcgaagc catggggttc aagatataag
      841 tcaggcgctg aatcggaacg gtgaggccga agatttcttt cgaagcgggc agtaaactca
      901 catcccaaat aatcggggtc agaaagaacc aggcgaggat cgccacttcc atgatctgct
      961 ggacatcgcg atagaaaaca ttcgtcgccg ccaggaacaa actcacgccc atcgaaaaga
     1021 tcatctgcac caggatgacc agcggcagaa aaacgatcca accggtcagc tgcacaccga
     1081 ggatcaacga caagacgatc aacaccggca gcgccatcag aaaattgatc aagctggaca
     1141 gcgtcagcga gatgggcagg acttcgcgcg ggaaatagac tttcttgatc aaatgtgagt
     1201 tatcgacaat gctgcgcgtg ccgcccatga tcgaggccga gaaaaaattc cacggcagca
     1261 ggccggacaa aatgaaagcc ggataatcat gccgattgct ctggccgagc atgaccgtat
     1321 agaccagcgt caagaccagc atcatcaaca aaggattgag cagcgaccag gcaatgccca
     1381 gtaccgaatt tttatagcgc acttttagat cgcgcacggt caggttgtaa accaattcgc
     1441 gataacgcca caactcagcg agtttcgcca gcatgcttgc cttctctaat tttagatagc
     1501 gctgccgcgc gatcggcgga tcgcggcaga cggatagcat catacacgga caaaatttga
     1561 tcgcccgctt catcccaact aaaacgatcg atcgcgcgct ggcgcagtgc ttgaccgatt
     1621 gtgctgcgcc gatcgggttt ctgcagcaga tcgattatac aaccagctaa agcgtccgcg
     1681 ctgccccgat cggcatacgt gcccctgtcg gccataaatt cgcgcgccac aggcatatcg
     1741 aacgcaatcg tcggtagcgc gcaggccatg taattcaata tcttgcccgc gccttcagtg
     1801 agcgacagtt tcggcgcgag ggccacatcg cccaggcgca aatagcgtgc ggcttgttcg
     1861 tacggcagac gaccccaaaa agtcatgtga tcgcctaagc ccaattgatt ggcatgcact
     1921 tgataaccga gatgccccgg atagcccatg atcaaaaaat gcgcgttggg ctgcgcctcg
     1981 atcactttcc tggccgcttg gataatgtga tcggtgcctt gataatgcgc cagcaaacca
     2041 agatagatca ccacggatcg atcgagcgga atacctaact gctctttcag cgaatcaatg
     2101 tcggcttgat cggctgcgct gcgcggccga aaaaattcgg tgtccacaca atcgggcagc
     2161 ggaatcactc gatcgggctg tagattgaat tcattcagca gcaccttcgc gctgtgctgc
     2221 gaggaagtca agatcgcgcg cggcatccga tcgatcgcgt tctccaat
//
LOCUS       DASRTJ010000346         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_20557,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000346 DASRTJ010000000
VERSION     DASRTJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20557"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFF70_07045"
     CDS             <1..190
                     /locus_tag="VFF70_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44489.1"
                     /translation="SRTMGQLLNGDRLDGSEIDLIEFVPYNGQATYDILPSSETGFYW
                     ANGVLVKSTLASSSIESR"
     gene            377..1591
                     /gene="xseA"
                     /locus_tag="VFF70_07050"
     CDS             377..1591
                     /gene="xseA"
                     /locus_tag="VFF70_07050"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZY44490.1"
                     /translation="MQPSLFTQPEALSVTDLTRYIRDVFELDYRLQDVWVQGEISNLS
                     RPSSGHVYFSLKDSGASIRCVMWKTAAATQASKLREGDAIIAHGKISVYEPQGAYQLY
                     VDAVQAAGAGDLFQQFELLKAKLQAEGLFDLDRKRPLPEAAHTIGIVTSPTGAALQDM
                     LNIIRRRWPLMKIVLASTPVQGDDAPVKIIAALKQLYRRTDLDAIIVARGGGSIEDLW
                     AFNDENVARTIVQSPVPVISGVGHEVDFTIADFVADVRAPTPSAAAELITPDQAETRS
                     MLQSYSYTMTSLLTSRLQNDRMILNSQVRALQHLSPRVKLSNARQRIDDLLASLQESM
                     AHDLELQREKISSLSAQLSALNPLNVLARGYAVVRIEPSGQIVRSRKQIKPQQPLSIR
                     VRDGEFDATSKG"
     gene            1595..1804
                     /locus_tag="VFF70_07055"
     CDS             1595..1804
                     /locus_tag="VFF70_07055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZY44491.1"
                     /translation="MKNAAKTKAIEGLTFEQAFKELEESVTKLESGELPLEEALTLYE
                     RGQALAAYSGMLLDAAELKIEKLGE"
     gene            complement(1842..>2040)
                     /locus_tag="VFF70_07060"
     CDS             complement(1842..>2040)
                     /locus_tag="VFF70_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44492.1"
                     /translation="MSIGVIVFLNFTINTGVLIRGLGSMVIGAAIFAISAWLLHVEEL
                     RLIMSLVVRRTKRSAENKNAD"
BASE COUNT          488 a          544 c          545 g          463 t
ORIGIN      
        1 cagcagaaca atggggcagt tacttaacgg tgacagactt gatggctcag aaatagacct
       61 cattgaattt gtgccttaca atgggcaagc aacatatgac attttgccaa gcagtgaaac
      121 cggtttttat tgggctaatg gagttttggt gaaaagcact ttagcttcct cttctataga
      181 aagccgttga catttctcga tctccgcgat gatcaattga gattgcttcg gcgcaaaagg
      241 cgcgccttgc aatgacgttc agattaaaaa cctccgcgct ctctgcgttc tccagtacgc
      301 actgcgtgct ttaccgcatg tgtgcggtta attcttttca tttgcctctc aactcgaaac
      361 agactacaat ctccgcatgc agccaagcct cttcacccaa cccgaagcgc tcagcgtcac
      421 cgatctcacc cgctacatcc gcgacgtgtt cgagctggac tatcgcctgc aggatgtgtg
      481 ggtgcagggc gagatcagca acctcagccg tccgtcgagc gggcacgtct acttctcgct
      541 gaaagattcc ggcgcatcga tccggtgtgt gatgtggaag accgccgccg cgacgcaagc
      601 gagcaaactg cgtgaaggcg atgcgatcat cgcgcatggc aagatcagcg tgtatgaacc
      661 gcagggcgcg tatcaactct acgtcgatgc ggtgcaagcc gcgggtgcgg gcgatctctt
      721 tcagcaattc gaactgctca aagctaaact gcaagccgaa gggctattcg atctcgatcg
      781 taaacggccg ctgcccgaag ccgcgcacac gatcggcatt gtgacttccc cgaccggcgc
      841 ggcgctgcaa gacatgctca acatcattcg acgccgctgg ccgttgatga agattgtcct
      901 ggcgtcaacg ccggtgcaag gcgacgatgc tccggtgaaa attattgctg cgctcaagca
      961 actttatcga cgaactgatc tcgatgcaat tattgtcgcg cgcggcggcg gatcgatcga
     1021 ggacttgtgg gcgttcaatg atgagaatgt agcgcgcacg atcgttcaat ctcccgtgcc
     1081 ggtcatttcg ggcgtgggcc acgaagttga tttcaccatc gccgattttg tggccgatgt
     1141 gcgcgcgccg acgcccagcg cggccgccga attgatcaca cccgatcaag cggaaacccg
     1201 atcgatgctg caaagttatt cgtatactat gacttctctc ttgacgtcgc gcttgcaaaa
     1261 cgatcgaatg atattgaact cgcaagtgcg tgcactgcag catctttcgc cgcgggtgaa
     1321 actctcgaac gcgcggcagc gcatcgatga tctgctcgcg tcgctgcaag aatcgatggc
     1381 gcatgatctg gaattgcagc gcgaaaagat ttcttctctg tctgcgcaac taagcgcgtt
     1441 aaatccgttg aatgttttag cgcgtggcta tgcggtggtg cggatcgaac cgtcgggcca
     1501 gatcgtccga tcgagaaaac agatcaaacc ccagcagccg ttatcgatcc gcgtgcgtga
     1561 tggggaattc gatgcgactt ctaaaggatg aagcatgaaa aatgcagcca agactaaagc
     1621 gattgaaggt ttaaccttcg agcaagcatt caaagaactc gaagaaagcg tgacgaaatt
     1681 ggaaagtggc gaactgccgc tggaagaagc gttaacgtta tatgagcgcg gccaagcctt
     1741 ggccgcgtat agtggtatgt tgctggacgc agccgagtta aagatcgaaa aattgggcga
     1801 gtgacgctca cctctgtcct ctcttcttgt tataagcagg tttaatccgc gtttttattt
     1861 tcagccgacc gttttgttct acggacgacg agactcatga tcagccgcag ttcttccacg
     1921 tgcagcaacc acgcggaaat cgcgaaaatg gccgcgccga tcaccatcga tccgaggccg
     1981 cgaattaaca cgcccgtgtt aatagtgaaa ttcaagaaga cgattacccc gatcgacatc
//
LOCUS       DASRTJ010000347         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_323385,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000347 DASRTJ010000000
VERSION     DASRTJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323385"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFF70_07065"
     CDS             <1..194
                     /locus_tag="VFF70_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44493.1"
                     /translation="LFLRHVNKAQTTFNPQQNYCVDYIGQIDNRAQFRQAIRVARGIE
                     FAACVTDRVQIILAVMAFD"
     gene            226..717
                     /locus_tag="VFF70_07070"
     CDS             226..717
                     /locus_tag="VFF70_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY44494.1"
                     /translation="MGSRRWTRLDLLTAMDEIWDNYLKRLSAMPIKQQTAYAKRNGYS
                     QPKELIAHLAAWIAETVRVMPYLCRDKHLPRDDTNDDVFNARVIERCRRLSRAEVEAE
                     FRRQRLALKKLIAALPDDALQVTRVYRWLSETIISHYQDHQLPEWSLVQLRHGKQSNR
                     GSV"
     gene            714..1628
                     /locus_tag="VFF70_07075"
     CDS             714..1628
                     /locus_tag="VFF70_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZY44495.1"
                     /translation="MITPNLIVHGGAWDIPDQEVAGHLLGTQAAAEIGWSILRYGGSA
                     LDAVEAAIRSMEDDPSFDAGRGAWLNMAGEIELDAIIMSGTDLSSGAVAAVQHIRNPI
                     SLARLVMEHSPHALIAGIGAERFAQAQGLQLVDEAELLTGQELERWHAIKKQPGFSIK
                     AAFAGDERPYGTVGAVAIDSHGHIACGTSTGGTPNKLQGRVGDSPLIGCGAYADDLSA
                     GASATGWGESIMKVVLCKSACDFAAQVLNAQSAADKAIGLLADRAHGVGGVIVIDRSG
                     QIGCAYNTPKMARAWIENDRVIAKVERW"
     gene            1640..1819
                     /locus_tag="VFF70_07080"
                     /pseudo
     CDS             1640..1819
                     /locus_tag="VFF70_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
     gene            1953..2591
                     /locus_tag="VFF70_07085"
     CDS             1953..2591
                     /locus_tag="VFF70_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HZY44496.1"
                     /translation="MKNVSNQIGCTIQLLVVLLILIVALVVIQLASGFPGSLFNQPQA
                     TPVIFVQPSVIDQVKPLGQLHTASYFLSTVVDTQMHVGVLNQLQRVLLVACGKVNAGV
                     DLQKMTAKDIKITGDKVNVHLPDPEIFDALLFDDRQCTYVVLRDEGILLPPNTQLESA
                     AREAAVANFRATALQNDILNTALQNAKTQLRSLIILLGYTQVDFTPESFSND"
     gene            2694..4649
                     /locus_tag="VFF70_07090"
     CDS             2694..4649
                     /locus_tag="VFF70_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZY44497.1"
                     /translation="MDDTFHFGGEIVWRPTPELIQNSRLKKFMDQYDIPDFQSLMHRS
                     TTDLDWFWNAVLKDLDIQFYKPYSNIVDLSKGIEWPVWCVDGVMNIVHNCLDKYIGTP
                     KENHAALIWEGEDGLSQQYTYGQLYREVNKCANALRSIGLKKGDAIGLYLPMIPEIVI
                     ALLAIAKIGGIILPLFSGYGETAIVSRLKDADAVALFTADGFDRRGQVVNMKQTADAA
                     AAQVSTLKHMIVAKRANADIAMFAARDHWWHELIDPQSDQSPTEHTSAEDPLMILYTS
                     GTTGKPKGALHTHCGFPIKAAQDMAHGLDLHPDEILYWMTDMGWMMGPWLVFGTLILG
                     ATMMIYDGAPDYPAPDRLWALIEKHKVTALGISPTLVRALIKFGDAPIKKHDVSSLRK
                     FGSTGEPWNPDPWLWLFNTVGGGQLPIINYSGGTEISGGIVMGNVLLPLKPCAFSAPV
                     PGMAADVVNEHGNSIRNQVGELVIRQPWIGMTRGFWHAPDRYIQTYWSRFPKMWVHGD
                     WAAIDNDGLWYILGRSDDTIKVAGKRLGPAEVESILVSHPDVSESAAIGVPDPIKGQA
                     VVCFCVLKPTAIASDQLRETLKDLVANELGKPLKPNEIKFVKDLPKTRNAKVMRRIIR
                     SAYLGQELGDTSSLENPSAVEEIKRSV"
     gene            4719..>5801
                     /locus_tag="VFF70_07095"
     CDS             4719..>5801
                     /locus_tag="VFF70_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44498.1"
                     /translation="MQSIDITRQSDQRKIWVIIAGTLALLIAAIIIIPGLSEYSMWFD
                     EVQVFRAADQLNTLPDFYQFADFPARIVHPPFYFALLKVWMIFVGQTDVVLRTLTTLF
                     GVLSAAFVYRVTVDLSHQPAAGLAAAIVFSSMGFASYYIHETHNYSLLMTESVAMLFF
                     YHRWQDRPQHRYAIGIVLTELMLIYTNYASVYLLLAFNLYALLVGFRRIRPWIMLQII
                     VVLGYLPWLPALHWVITSGYSTTEAHGVVATSMLTNWSTIPVLMQSLLYDGWSYYAVF
                     LVIGLIASVAWPLQSRSQGLRAVGLLLLVAGASILFALIGNTVVQTLTARRVIYLLVF
                     VAIVLGYGFAHLPKRLRWLILAAFLIM"
BASE COUNT         1345 a         1490 c         1605 g         1361 t
ORIGIN      
        1 tcctttttct caggcatgtc aataaagcgc agacaacatt caacccgcag caaaactact
       61 gtgtggacta catcgggcag attgataacc gcgcgcaatt tcggcaggct attcgggttg
      121 ccaggggtat tgagtttgct gcctgtgtta ctgatcgtgt gcagatcatt ttggcagtca
      181 tggcgtttga ttgaagactc tcaatcttcg acgccaggag gttaagtggg ctcaagacgt
      241 tggactcgtt tagacctgtt aacagccatg gatgaaattt gggacaatta tttgaaacga
      301 ctctcggcaa tgccgatcaa acaacaaacc gcttatgcga aacgaaatgg ttattcgcag
      361 cctaaagaat tgattgctca cctcgcagcg tggatcgcag agacggtgcg tgttatgcca
      421 tatttatgcc gggacaaaca cctgccgcgc gatgatacga atgacgacgt gtttaacgct
      481 cgcgtcattg aacgctgccg cagattgtcg cgcgccgaag tcgaagcgga attccgacgg
      541 cagcgactcg ccctcaagaa attgatcgct gcgttaccgg acgatgcgct tcaggtcacc
      601 agggtttacc gatggttatc ggagacgatc atcagccatt atcaagatca tcagttgcca
      661 gagtggagtc tcgttcagtt gaggcacgga aaacaatcta atagaggatc ggtatgatta
      721 ctcccaattt gatcgtgcac ggcggcgcgt gggatattcc tgatcaagaa gtggctggac
      781 atttgctcgg cacacaagcc gcggccgaga tcggttggtc gatcttgcgc tatggcggat
      841 cggcactcga tgccgtggag gccgcgatcc gatcgatgga agatgatccg tcattcgatg
      901 caggccgggg agcgtggtta aatatggcgg gcgagatcga actcgatgca attataatga
      961 gcggcacaga cttaagcagc ggcgcggtag cagccgtgca gcacatccgc aatccgattt
     1021 cactcgcacg gttggtcatg gagcacagtc cgcacgcgtt gatcgccggt atcggcgcgg
     1081 aacggtttgc ccaagcgcag gggttgcagt tagtcgatga ggcagaatta ttaacagggc
     1141 aggaattaga acgctggcac gcgatcaaga agcagccagg cttttcaatt aaggcagctt
     1201 ttgccggtga cgaacgaccg tatggcacgg tcggcgcagt ggcgatcgat agtcacggcc
     1261 acatcgcatg tggcacatca acgggtggca caccgaacaa attgcaaggt cgcgtgggcg
     1321 atagtccgtt gatcggttgt ggcgcgtatg cagatgatct gagtgctggc gcgtcagcga
     1381 caggctgggg cgaatcgatc atgaaagtgg tgctgtgtaa atcggcctgc gattttgccg
     1441 cgcaagtgct gaacgctcaa tcggcggccg acaaagcaat cgggttactg gccgaccgcg
     1501 cacacggcgt cggcggagta atcgtgatcg atcgttcagg ccagatcggt tgtgcgtata
     1561 atacgccgaa gatggcacgt gcgtggattg aaaacgatcg cgtaatcgcg aaagttgaac
     1621 gatggtgagt aatgcgtgac ccgtaaccgg cggcttgtcg tatcgcgaag tgcaattgtt
     1681 gatggagatg ctggccgagt ccggcaaggt cacatcgatg catctggtga aaatcaatcc
     1741 gattttggat cagcgcaatc gcactgcgga gttggcagtc ggcttaacga caagtttgtt
     1801 ggggaagaga cttatgtaat tcaccatata ccgcctatgt cttttgtaaa ggatcaatcg
     1861 gaatcgaatg agggctaaat caaacaattc gatcggtcgg tgtggtatca taagcttaac
     1921 atcgaacctt gcgtaacaca gagcgataac caatgaaaaa tgtaagcaat caaattggct
     1981 gcacgattca actccttgtg gtgctgctga ttctcatcgt cgcactggtc gtgatacaac
     2041 tggcgagcgg ctttcccggc agcctgttca accaaccaca agccacgccg gtcattttcg
     2101 ttcagccgtc ggtgatcgat caggtcaaac cgctgggtca gctgcatacg gccagttact
     2161 tcttatcgac ggttgtcgat acgcagatgc atgtcggcgt gctaaatcaa ttgcagcgcg
     2221 tgctgttagt ggcttgcggc aaagtgaatg ccggggtcga tctgcagaag atgacagcga
     2281 aagacatcaa gattaccggc gacaaagtca acgtgcactt gcctgatccg gaaatcttcg
     2341 atgccctgct cttcgacgat cggcagtgca cctacgtcgt gctccgcgac gagggcatat
     2401 tgctgccgcc caatactcaa ctcgaatcgg cggcgcgcga ggcagcagtc gccaatttca
     2461 gagccacggc tttgcaaaac gatatcctga acaccgcgct ccaaaatgcc aaaacccagc
     2521 tgcgcagtct gattatcttg ttgggctaca cgcaagttga cttcacgccg gaaagtttct
     2581 caaacgattg atgaacatgg ctaaaagtca cgcccgcgca agcgggtgtc cagttattcc
     2641 aatctggatt cccgcttgcg cgggaatgac gatgacatcc tgaggagact cgtatggatg
     2701 acacgtttca cttcggcggc gagatcgtct ggcgccctac ccccgaattg atccagaaca
     2761 gccgcttgaa gaagttcatg gatcagtacg acatcccgga ttttcaaagc ctcatgcacc
     2821 gatcgacaac cgacctcgat tggttttgga acgccgtatt gaaagacctc gatattcaat
     2881 tctataagcc atacagcaac attgtcgatc tgtccaaagg catcgagtgg ccggtatggt
     2941 gcgtcgacgg ggtgatgaac attgtgcaca actgcctcga caaatacatc ggcaccccga
     3001 aggaaaatca cgcggctctg atttgggaag gcgaagatgg cctcagccaa cagtatactt
     3061 acggccaact ttatcgcgaa gtcaacaagt gcgcgaacgc actgcgatcg atcgggctta
     3121 agaaaggcga cgcgatcggt ttgtatctgc cgatgattcc ggagatcgtg attgcgctgc
     3181 tagcgattgc aaagatcggc ggaattattc tgccgctgtt ttcgggctat ggcgaaaccg
     3241 cgatcgtttc acgcttgaaa gacgcggacg ccgtagcgct tttcaccgcc gatggtttcg
     3301 atcggcgcgg ccaagtcgtc aatatgaaac aaactgcgga tgcggccgcc gcgcaagtgt
     3361 ccacgctgaa acacatgatc gttgcgaagc gcgcgaatgc cgatatcgcg atgttcgccg
     3421 cgcgcgatca ttggtggcat gaattgatcg atccgcagtc tgatcaatcg ccgaccgagc
     3481 acacctccgc cgaagatccg ctgatgatcc tgtatacctc cggcacaact gggaaaccca
     3541 aaggcgcgct gcacacccac tgcggctttc cgatcaaagc cgcgcaagac atggcgcacg
     3601 gtctcgatct acatccagat gaaattttat attggatgac ggacatgggc tggatgatgg
     3661 ggccgtggct tgtcttcggc actttgatct taggcgccac gatgatgatc tacgatggcg
     3721 cgccggatta tcccgcgccc gatcggttgt gggcgctgat cgagaaacac aaagtcaccg
     3781 cgctgggcat ctcgcctaca ctcgtgcgcg cgttgatcaa attcggcgat gcgccgatca
     3841 agaaacacga tgtgtcatcg ctgcgcaaat tcggatcgac tggcgagccg tggaatcccg
     3901 atccgtggtt gtggctgttc aacaccgtcg gcggcgggca actgccgatc atcaattatt
     3961 ccggcggcac ggaaatcagc ggcggcattg tgatgggcaa cgtgctgctg ccgttgaaac
     4021 cgtgcgcgtt ctcagcaccc gtgcccggca tggccgccga tgtcgtcaat gagcatggca
     4081 actcgatccg caatcaagtc ggcgagttag tgatccggca gccgtggatc ggcatgacgc
     4141 gcggcttctg gcacgctccc gatcgatata ttcaaacgta ttggtcgcgc tttccaaaaa
     4201 tgtgggtgca tggcgattgg gctgcgatcg ataacgacgg cctgtggtac atccttggcc
     4261 gatcggatga cacgatcaaa gtcgcgggca agcggctcgg tccggcggaa gtcgaatcga
     4321 tcctggtgtc gcatcccgat gtgagcgaat ccgccgcgat cggcgtgccc gatccgatca
     4381 agggacaggc cgttgtgtgc ttctgtgttt tgaaaccaac cgcgatcgcc agcgatcaat
     4441 tgcgcgagac cttgaaagat ttagtcgcga atgaattggg aaagccgcta aagccgaacg
     4501 aaatcaaatt cgtcaaagac ttgcccaaga cccgcaacgc caaagttatg cgccgcatca
     4561 ttcgatcggc atatttgggt caagagttgg gcgatacgtc gtcgcttgaa aatccatcgg
     4621 cggtggaaga gatcaagcga tcggtgtgat gttggatgcc aacagaaatt gactaatact
     4681 tacttgtttc gcgcttcgct catcttgacg aaaagaatat gcagagcata gatataacta
     4741 gacaatctga tcaacgcaag atctgggtaa tcatagctgg tactctggca ctgttgatcg
     4801 ctgccatcat tatcattcct ggcctgtcag aatatagtat gtggtttgac gaagtgcagg
     4861 tattcagagc cgccgaccag ctaaatacgc tcccggattt ttatcaattt gcggatttcc
     4921 ctgctcgaat agtgcatcct cccttctatt tcgctctgct caaagtttgg atgatatttg
     4981 ttggccaaac cgatgtcgta ttgcgcacac tgactacctt gttcggcgtc ctatcggcgg
     5041 catttgtata tcgtgtcacg gtcgatttgt cacaccagcc ggccgcaggt ctggcggccg
     5101 cgatcgtttt tagcagcatg gggtttgcga gctattacat tcacgagacg cataattaca
     5161 gcttgctcat gaccgagtcc gtggcgatgc tgtttttcta tcaccgctgg caagatcgcc
     5221 cacagcaccg ctatgctatt ggcatcgtcc tgacggagct catgctaatc tacactaact
     5281 atgcatccgt gtatttgctg ctggctttca acctatatgc cctgctagtt ggattccgtc
     5341 ggatacgccc atggatcatg cttcaaatta tcgtcgtgct gggatactta ccttggctgc
     5401 ctgcactaca ttgggtcatc acctcaggct actcgacaac cgaagcacac ggtgtagtgg
     5461 cgaccagcat gttgacaaac tggtcaacaa tccctgtact gatgcagtct ctgctgtacg
     5521 atggatggtc atactatgcc gtctttcttg tcatcgggct gattgccagc gtagcctggc
     5581 cacttcaatc acgctctcag gggttgcgtg cggtcgggtt gttacttcta gttgccgggg
     5641 ccagcatctt attcgcttta attgggaata ccgttgtgca gactctaacc gcacggcgtg
     5701 tgatctacct cttggtattt gttgcaattg tcctgggcta tgggtttgct catctgccca
     5761 agcggttgcg gtggctaatc ctggcagctt tcctcatcat g
//
LOCUS       DASRTJ010000348         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_122208,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000348 DASRTJ010000000
VERSION     DASRTJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122208"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1564)
                     /locus_tag="VFF70_07100"
     CDS             complement(<1..>1564)
                     /locus_tag="VFF70_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZY44499.1"
                     /translation="IDLRSDRSFMRDVAAWERTPPRPARYAPFPTSLDRRLINILKSR
                     GIDQLYTHQAASIEATLRGENSVVVTATASGKTLCYNLPVLHTLLADPKATALYLFPT
                     KALAQDQLVALNELIVGLDKAIAVQTYDGDTSRERRKSAREAGGIIITNPDMLHTGIL
                     PHHTQWASLFESLRYIVIDELHTYRGIFGSHFANVLRRLKRICRFYGSNPQFVLASAT
                     IANPQQLAEKLIEEKVTLIDDDGAPRGEKHWIMLNPPLIDPALGQRRSMVLETRDIAA
                     KFLQADIQTIVFARARSTTEVLLTYLRDAIASEGGDPKTIHGYRGGYLAGERRAIEKG
                     LREGSIRGVVATNALELGVDIGQLEAAVLAGYPGTIASALQQSGRAGRRSNVSAAVIV
                     ASASPLDQFIVTHPRYFFERSVEHGLIAPDNLIILTNHIKCASFELPFDAGEKFGRFE
                     QTDELLEALVEEGILNKQTAARPNLPDRYFWMADQYPATGLSLRSGTADTIVIMDTTE
                     ARARSIGQIDRES"
BASE COUNT          351 a          439 c          436 g          338 t
ORIGIN      
        1 ctgactctcg atcgatttgg ccaatcgatc gggcgcgggc ctcggtagtg tccatgatca
       61 caatcgtatc tgccgtgcca ctcctcagcg ataaacccgt cgccggatat tgatccgcca
      121 tccagaaata ccgatcgggc agattaggcc gggccgcagt ttgtttgttt aagatacctt
      181 cttcaactaa agcctccagc aactcatcgg tttgttcgaa gcgaccaaat ttttcgcccg
      241 catcgaacgg caattcaaat gacgcgcact tgatgtgatt cgtgaggatg atcaaattat
      301 cgggcgcgat caaaccgtgt tcgaccgatc gctcgaagaa gtagcgcgga tgcgtgacga
      361 tgaattgatc gagcggcgag gcactggcta cgatcaccgc cgcactcaca ttcgatcgac
      421 gcccagcccg accagattgc tgcaatgccg aagcaatcgt gccgggataa ccggccagca
      481 ccgccgcttc gagttgacct atatcgacgc cgagttcaag cgcattcgtc gccaccacgc
      541 cgcgaatcga tccttcgcgc aagcctttct caatggcgcg gcgttcaccg gccaaatagc
      601 cgccgcgata accgtggatc gttttcggat cgccgccttc ggatgcgatg gcatcgcgca
      661 ggtaagtcag caggacttcg gtggtcgatc gggcgcgcgc aaaaacgatc gtttgaatat
      721 cggcttgcaa aaacttggcg gcgatgtcgc gtgtttccag caccatcgat cgacgctggc
      781 ccaacgccgg atcgatcaac ggtggattga gcatgatcca atgcttctcg ccgcgcggcg
      841 cgccgtcatc atcgatcaag gttacttttt cttcaataag tttctcagcc agttgctgcg
      901 gattcgcaat cgtggccgag gcgagaacaa actgcggatt cgatccgtag aagcggcaga
      961 tgcgtttgag acggcgcaag acgtttgcaa agtgactgcc gaagatgccg cgataagtat
     1021 gcaactcatc gatcacgatg taacgcagcg attcaaacag cgaggcccac tgagtatgat
     1081 gcggcaagat gccggtgtgc agcatgtccg gattggtgat aatgatcccg cccgcctcgc
     1141 gcgccgactt gcgccgttcg cgcgaagtgt cgccatcgta cgtctgcacg gcaatggctt
     1201 tatccaaacc tacgatcaat tcattcaacg ctacaagttg atcttgcgcc agcgctttcg
     1261 tcgggaacag atagagcgcg gtcgcttttg gatcagctaa gagagtgtga agtaccggca
     1321 agttgtaaca cagcgttttg ccggaagcag tcgccgtgac gacgaccgaa ttttcgccgc
     1381 gcaacgtcgc ttcgatcgag gccgcttgat gcgtatacag ttgatcgatg ccgcgtgatt
     1441 ttagaatgtt gatcaatctt cgatcaagcg acgttggaaa cggcgcatag cgcgccggtc
     1501 gaggcggcgt gcgttcccac gcggccacat cgcgcatgaa ggatcgatcg gagcggagat
     1561 caat
//
LOCUS       DASRTJ010000349         9429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_122211,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000349 DASRTJ010000000
VERSION     DASRTJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122211"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFF70_07105"
     CDS             complement(<1..1059)
                     /locus_tag="VFF70_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44500.1"
                     /translation="MTTNFSLLVTRYLRRIVYSVWPFAFLALLTSIFFWPMIFSGKFI
                     PHGGGDLASFLYPQYSFAAQSIRSGSIPLWNPHLYSGAPFLADNQAGVLYPINLLAFW
                     LFPNFSYQVMEWLVVLHIWIAGAAMYICLRMWPSLSRINGAQVGLGWGSALFGAIAFM
                     FSDVFVTHIGNLNLIAAAAWMPLAFGLLARGLHDRRAGKVIGSGIVFGLALLAGHAQM
                     SVITLIGLALIAVWQIVIAGSWRDRWRVIGLAALMLVVAFGLTAMQLLPSIELTRYSL
                     RSGLSYDEATAYSLPLTALASIFSPLLFGRGAIDFWGPWLRVETGYLGVIPLVLMLFA
                     FTRKNLRQVLFLASLGLVG"
     gene            complement(1066..3060)
                     /locus_tag="VFF70_07110"
     CDS             complement(1066..3060)
                     /locus_tag="VFF70_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44501.1"
                     /translation="MTIDERNRTLPLAPRTLEIIAVSIILLLAAFLRFNHLDWTEFKQ
                     DEAHLSQIAFDMARHGQIPLHSINASVGVANLPLGAWLLAIPYAATSSPIVATAFLAG
                     LNVLAVAACYAFARRCFKSSGAYSIVAALIAALLFATAPWAVLQSRKLWANNFFPIFV
                     VAWAWTGWLAFAEKRSRWLIVHAIVLALCLELHYYALMLVLVSTVWFSVFIRRVAWKT
                     VLIGLIAAIILFAPFIIADASLGWPTAAKLLALFNQPSTADSSSIDLGWLTATGLNIH
                     SLAGPQEFENYRALIPGFDIVFILEGVLMLIGLLVAAIDVARSIARRAWTVRSTVSLM
                     LATWLVIPIVSQISHRIPLYQHYFAILWPVPFLLIGLLVCRVSTSRVLRRVLIGGVIV
                     IAFVQTYAMLTLQQFVASRPTPGGAGIPIGYYESIVNQAKFALSNHHASEIVINTVGA
                     DPQLDEYPAIFDFLLNGVPHRFVDASQSMRVYPLQPSIQIDYSPAALLTDMPVRDQIS
                     TIDLRVGEQPAQIYESSGNGALPCEATSATRWANGTTFLAAQIDPLIAGQSALIHVCV
                     RLDQNSPSTDYHWTNQLFDKAGKRWAQVDSAGFPSHDWRAGDGVLLTFNIDLPADMPA
                     GDYILRIGQYTYPDIASVPVIDVMNNPQSDAIEIPVQVNR"
     gene            complement(3088..3606)
                     /gene="mog"
                     /locus_tag="VFF70_07115"
     CDS             complement(3088..3606)
                     /gene="mog"
                     /locus_tag="VFF70_07115"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HZY44502.1"
                     /translation="MINQPRVGILIISDRSSTGERADASGPLIRDFVLEKLQWSIDVT
                     LIVPDDSEKIQAALIDWCDSHKLNLILTTGGTGFAPRDVTPEATRSIIEREAPGLAEA
                     MRAASRTQTPHAMLSRAVCGLRGLTLIVNLPGSPKAVRENLETILPALPHAIDLLREA
                     PDTEAQHAKRVA"
     gene            complement(3684..5420)
                     /locus_tag="VFF70_07120"
     CDS             complement(3684..5420)
                     /locus_tag="VFF70_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HZY44503.1"
                     /translation="MAQRKTKEPQPQRQWRRIDLHLHTPASIDFQESSVTYLDVLQTA
                     EARGLDVIAFTDHNSVAGYAHMLREIDQLELLEKLKRIQAEERQRLEEYRRLRKKIVV
                     LPGFEFTATFGFHIIGVFSEDTSAREIEHILLNLNVPSEKLDLGATDCGATADVLTAY
                     RLIDEAGGLVIAAHVNSSNGVAMRGLGFGGQTKIAYTQDQHLHVLEVTDLDSTRRGST
                     ATFFNGSKPEYPRRMHCIQGSDSHRLTRDQKNPKNLGIGERVTEVQLAANSFEALRDV
                     FLGNDFTLTRQYSPKHEPIDVIRAAREEGPNIVQAFHEGYSQRGGKLRAILADVCAFA
                     NTNGGTIYIGIPDDLAKPAQGVGNPNQMIQSLEKAIESSITPSVEVSFATHPSGKIKV
                     VQINVPRGMDVPYAIEDNKIYVRSEAETNLAVRDEIVQIIQRRTGKPDNSIVPVSIES
                     SNEANGQIAAPRTGVEVVETSLRDGVTYHIVRDLRNGNLVHNVTRKSARKLWHYAIIE
                     HEKFQSGSVKVKWLSDLGLIGVSRRLGVTRYDFVQRQADGKLRAYYGVTDDGIHGEWR
                     RVAELAESSKVI"
     gene            complement(5420..5782)
                     /locus_tag="VFF70_07125"
     CDS             complement(5420..5782)
                     /locus_tag="VFF70_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44504.1"
                     /translation="MIRILIVDDDRATSTLLRTLFEIEGYEGVVCPRPDEVLQTAERE
                     RPDLILMDVHLAEVDSIGILKTIRTWPALKETPVIMMSGLDVEDKCLKAGATAFVLKP
                     FRPAELLAEIKRNVKTEK"
     gene            complement(5908..8505)
                     /gene="lon"
                     /locus_tag="VFF70_07130"
     CDS             complement(5908..8505)
                     /gene="lon"
                     /locus_tag="VFF70_07130"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZY44505.1"
                     /translation="MTDEYNASLGNQIFRPETLDIALLADADADAISPDKSIGSTLPD
                     DLPILPLRGVVVYPLTAVPLTVGQARSVQLIDDAMLQKKVIGLVASKDPSIEEPGPDQ
                     CYTIGTTATVQRLLRLPDGTIRLLVLGLERIRIKEFTGTEPYLKARVESAPEQSEKSV
                     EVEALRRNIGSLFGRLVELVPGIPDEVAAVAINIEDPRQLAYAIATYMRIELADAQEI
                     LELDDVTAKLRKIVALLNKELEVLELGHKIQTEAHSEMEKVQREYYLREQLKAIQKEL
                     GEHDEQQMEVEELRQKIEEAAMPEEAHKESVRELDRLSRLPTAAAEYGVIRTYLDWLV
                     NLPWSKITPDNLDIAHARKILDEDHYGLQDIKERILEYLAVRKLKSERANVEDDTSAG
                     ETKDMIRREREGVILCFVGPPGVGKTSLGQSIARAMGRKFVRQSLGGVRDEAEIRGFR
                     RTYIGALPGRVIQALRRIETRNPIFMLDEVDKLSSDSRGDPSSALLEVLDPEQNREFR
                     DHYMDLPFDLSQVIFICTANYLENIPGPLRDRMEVIQLSGYTDQEKIKIAQGYLVPRQ
                     IKENGLRKSEVSFTDAAIFQIIHDYTREAGVRNLERELGRVCRKLATQIAEGVSTEMQ
                     VDTPDVVKYIGKPHFYGEIAERTHLPGVATGLVWTPVGGDIIFIEATQMSGARGFQLT
                     GQLGDVMRESAQAAMSFVRSHAETLGVSDKFFATHDIHLHVPAGAVPKDGPSAGVTMA
                     TALASLLTGRSVRDDLAMTGEITLRGQVLPVGGIKDKILAAHRLGLRTIILPKRNEKD
                     LDDLPEEIRQSMKYILVDQVEQVFDAALSGAQQRLTDTSGTHAVKHAVRTEARSADRR
                     ALARKRKTK"
     gene            complement(8572..9018)
                     /locus_tag="VFF70_07135"
     CDS             complement(8572..9018)
                     /locus_tag="VFF70_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZY44506.1"
                     /translation="MSMSYSEPREPADRSQSDLERRHHHQLGSGSWIVWEHTHAWQPP
                     TDVYEIDDAVVVRIEIAGMHETDFKVFVQDQLLIVTGRRSDPSPKVAYHQLEVRYGEF
                     RCEVYLHWIVDEAGILATYHNGFLEVHLPKAGSRRVQVVDLGLRNL"
BASE COUNT         2298 a         2705 c         2402 g         2024 t
ORIGIN      
        1 accgaccaaa cctaaagaag ctaaaaacaa aacttggcgt aaatttttgc gcgtgaaagc
       61 aaacaacatc aacaccaagg ggataacacc cagataacct gtctcgacgc gcaaccacgg
      121 cccccaaaaa tcgatcgcgc cacggccaaa caataatggc gaaaagatcg acgccaaggc
      181 tgtcagcggc aaactgtacg cggtcgcttc atcgtacgac aagccggacc gcaacgaata
      241 gcgcgtcaac tcgatcgacg gcaaaagctg catcgccgta aggccaaagg caacaactaa
      301 catcaatgcg gctagtccaa tcactcgcca tcgatcgcgc caactcccgg cgataacgat
      361 ctgccacacg gcaatcaacg ctaatccgat caaggtgatc accgacatct gtgcatgccc
      421 cgcgagcaac gccagcccga agacgattcc gctcccgata acttttccgg ctcgacggtc
      481 atgcaagccg cgtgccaata atccaaaagc caatggcatc cacgccgcag ctgcgatcaa
      541 attcaagtta ccgatgtgcg tcacaaacac atcactgaac atgaacgcga ttgcgccaaa
      601 caaagcggac ccccacccta gccccacttg cgcgccgttt atacgggaga gggaaggcca
      661 cattcttaaa catatgtaca tcgccgcgcc tgcgatccag atgtgcagga cgactaacca
      721 ctccatgact tgataagaga aattggggaa caaccagaac gccagaagat tgatcgggta
      781 tagcacgccc gcttggttat cggccagaaa gggcgcgccc gaatacaaat gcggattcca
      841 caacgggatc gatccggatc gaattgactg cgcggcgaag gaatattgcg gatacaagaa
      901 cgaggccaga tcgccgccgc catgcggaat aaattttcca gagaatatca tcggccagaa
      961 gaagatcgat gtgaggagtg cgagaaaagc gaatggccac acggaataca caattcgccg
     1021 cagataacga gtaacgagta acgagaaatt tgtcgtcatg aataattaac ggttgacttg
     1081 caccggaatc tcgatcgcat ctgactgagg attgttcatc acatcgatca ccggcactga
     1141 agcaatgtcg ggataagtgt attggccgat acgcaaaatg taatcgccag cgggcatgtc
     1201 agcaggcaga tcgatattga aggtcaacag caccccatcc cccgcgcgcc aatcatgtga
     1261 cgggaagccc gcgctatcga cttgcgccca gcgcttgccc gctttatcaa agagctgatt
     1321 cgtccaatga taatcggtgc tgggcgaatt ttgatccagc cggacacaca cgtgaatcaa
     1381 tgccgactgt ccggcgatca gcggatcgat ttgagcggct aaaaatgttg tgccgttcgc
     1441 ccagcgagta gcagaagtcg cctcgcaagg cagcgcacca tttccacttg attcgtaaat
     1501 ctgcgcgggc tgctcaccga cgcgcaagtc gattgtagaa atttgatcgc gaacaggcat
     1561 atcggttagc aacgcggctg gcgaataatc gatctgaatg ctgggttgca gcggatacac
     1621 tcgcatcgat tgcgaagcat caacaaatcg atgcggcaca ccattcaaca agaaatcaaa
     1681 aatggctgga tactcatcca gctgcggatc ggcaccgacg gtattgatca cgatctctga
     1741 cgcatgatgg ttgctcaacg cgaacttggc ttgattcacg atcgactcgt aataaccgat
     1801 cgggattccg gccccgccgg gcgtggggcg tgatgcgaca aattgctgca aggtaagcat
     1861 cgcgtaagtc tgcacgaacg caatcacgat cacacccccg atcaacactc tccgcagcac
     1921 gcgcgatgtt gatactcgac agacgagcag accgatcaaa agaaatggta caggccagag
     1981 aatcgcgaag taatgctgat ataaaggaat gcgatggctg atctgactca cgatcgggat
     2041 caccagccaa gtcgccaaca tcagacttac cgtcgaacga acggtccaag ctcgacgagc
     2101 gatcgagcga gccacatcga tagccgcgac cagcaagccg atcaacatca acacgccttc
     2161 aagaatgaat acaatatcga agcccggtat cagcgctcgg taattttcaa attcctgcgg
     2221 cccggctaat gaatgaatat ttagcccggt agccgttaac cagccgagat cgatcgagga
     2281 tgaatcggcc gtcgagggtt gattgaacag cgcgagcaat ttagcggcag tcggccagcc
     2341 aagcgaagca tcggcaatga taaaaggcgc aaacagtatg attgcggcaa tcaaaccgat
     2401 caagaccgtc ttccacgcaa cgcgccgaat gaacacactg aaccacaccg ttgataccag
     2461 caccagcatc aaagcgtaat agtgcagttc aaggcacaac gccaaaacga tcgcgtgaac
     2521 gatcaaccag cgcgatcgtt tttcggcaaa ggctaaccag cccgtccacg cccaggcaac
     2581 gacaaaaatt ggaaagaaat tattcgccca caatttacgc gattgcagca cggcccacgg
     2641 cgcggtcgca aacaatagcg ccgcgatcaa tgcagcgacg atcgagtaag cgcccgatga
     2701 tttgaaacag cgtcgagcga aagcgtagca tgcggccacc gctaaaacat tcaagcccgc
     2761 caagaacgca gtcgcaacga tcggactgga tgtggccgca taagggatcg ccagcagcca
     2821 tgcgcccagc ggcaggttcg ccacaccgac cgaggcattg atgctgtgca gcggaatctg
     2881 tccgtgccgc gccatatcga acgcgatctg cgacagatgc gcttcgtctt gtttgaattc
     2941 agtccagtca agatgattga agcgaaggaa ggcggcgagg aggagaatga tacttacagc
     3001 aataatttct aacgtgcgag gggcgagggg taaggtgcga ttgcgttcat cgatagtcat
     3061 tactcattac tcgttacttt tcacgcccta cgcaacacgt ttcgcatgct gcgcttctgt
     3121 atcgggcgct tctctcagca gatcgatcgc atgcggcagc gcgggcaaga tcgtttcgag
     3181 gttttcgcgc acggccttgg ggctgccggg cagattgacg atcaacgtca ggccgcgtag
     3241 gccgcacacc gcgcgcgata acatggcatg tggagtttga gtacgactag cggcacgcat
     3301 cgcttcggcc aggccgggcg cttcacgctc aatgatcgat cgcgtcgctt cgggcgtcac
     3361 atcgcgcggc gcgaagccgg tgccgcctgt tgtcaagatg agattcaatt tgtggctgtc
     3421 gcaccaatcg atcagtgccg cttgaatctt ttccgaatcg tcgggcacta tcaatgtgac
     3481 atcgatagac cattgcaact tctcaagcac gaagtctcga atcaagggcc cgctggcatc
     3541 agcacgttcg cccgttgaac tgcgatcact aatgatgaga ataccaacgc gcggttgatt
     3601 aatcatccca agacgctcaa ggcggacaac agtccgccgt taactttccg ccattttatc
     3661 aaccggcggg agcattcata acttcaaatt acttttgatg attcagcaag ttcggccact
     3721 ctgcgccatt caccgtgaat accgtcgtcg gtcacgccat aatatgcgcg cagtttccca
     3781 tcggcttggc gctgtacgaa atcatagcgc gtcacgccca agcgcctcga gacaccgatc
     3841 aagccgagat cgctcagcca cttcactttc actgatcccg attggaattt ttcatgctcg
     3901 atgatcgcat aatgccacag cttgcgcgct gatttgcgcg tgacgttgtg gaccagattg
     3961 ccattgcgca gatcgcggac gatgtgatac gtcacgccat cgcgtaacga agtctccacc
     4021 acttcaacgc ccgtgcgcgg cgcagcgatc tgtccgttgg cctcgtttga actttcgatc
     4081 gaaaccggca cgatcgagtt atcgggtttc ccggtgcggc gttgaataat ctgcacgatc
     4141 tcatcccgca ccgccagatt ggtctcagcc tcgctgcgca cgtaaatctt gttgtcctcg
     4201 atcgcgtacg gcacatccat gccgcgcggc acattgattt ggacaacttt gattttgccc
     4261 gatggatgcg tggcgaaact cacctcgacc gacggtgtga tcgaactctc gatcgctttt
     4321 tcaagcgatt gaatcatctg gttcggattg ccgacgccct gcgccggctt cgctaaatca
     4381 tcggggatac caatatagat cgtgccgccg ttggtatttg caaaggcgca tacatcggcc
     4441 aggatcgcgc gcagcttgcc accgcgctga ctgtagcctt catgaaacgc ctgaacgata
     4501 ttcgggcctt cctcgcgcgc cgctcgaatc acatcgatcg gttcgtgctt cggactgtat
     4561 tgacgcgtca gcgtaaaatc gttgccgaga aacacatcgc gcagcgcttc aaaactgttg
     4621 gcggcgagct gcacttcagt gacgcgctcg ccaataccta aattcttcgg attcttttgg
     4681 tcacgcgtta aacgatgcga gtccgagcct tgaatgcagt gcatccggcg cggatactca
     4741 ggctttgaac cattgaaaaa tgtggcggtc gatccgcgtc gcgtgctgtc cagatcagtc
     4801 acttccaaaa cgtgcagatg ctgatcctgc gtgtaggcaa tctttgtctg accaccgaat
     4861 ccaagacccc gcatcgccac accgttgctg gagttcacat gcgcggcgat caccaatcca
     4921 cctgcttcgt cgatcaaccg ataagccgtc aatacatcgg cggtggcgcc gcaatcggtc
     4981 gcgcccagat cgagtttttc agacggcaca ttcaagttca acaagatatg ctcaatctca
     5041 cgggccgaag tgtcttccga aaagacgccg atgatatgaa agccaaatgt ggccgtgaat
     5101 tcaaagccgg gcagtactac aatcttttta cgcagccggc gatattcttc caagcgctgg
     5161 cgctcttccg cttggatacg cttcaatttc tccaaaagtt ccagctgatc aatctcacgc
     5221 agcatatgcg cgtaaccggc cacgctgttg tgatcggtga acgcaatgac atccagcccg
     5281 cgtgcctctg cagtctgcag cacgtcgaga taggtgaccg aagattcttg aaaatcaatt
     5341 gaggcgggtg tatgcagatg cagatcgatc cgccgccact ggcgttgagg ttgtggttct
     5401 ttcgttttac gttgagccat tatttttccg tcttcacatt gcgttttatc tcagccagca
     5461 attcagccgg ccggaacggc ttcagtacaa acgcggtcgc accggctttc aagcatttgt
     5521 cctccacatc caagccggac atcataatca ccggtgtctc tttcaacgct ggccatgtac
     5581 ggatagtttt cagaatacca atgctgtcca cttcggccag gtgaacgtcc atcagaatta
     5641 aatccgggcg ctcgcgctcg gccgtttgca gcacttcatc aggccgcggg cagaccacgc
     5701 cctcgtaacc ttcaatctca aacaacgtgc gcagcaaagt ggatgtagct cggtcatcgt
     5761 cgacaattaa aattcgtatc acaagcatcc tcgctctgcg ggccacagga ttcgatcggg
     5821 cgctggcaga ttgatggtac gcactgcgta ctttaccgca tgtgtaccgc aggtgtcggg
     5881 ttgatcagga tccgccaacg gcgtcgatca ttttgtcttg cgtttgcgcg ccagtgctcg
     5941 acggtccgca ctgcgtgctt cggtacgcac tgcatgcttt accgcatgtg taccactggt
     6001 gtcggttaat cgctgctgag cgcccgataa agccgcgtca aaaacttgct ctacttgatc
     6061 gaccaaaata tacttcatgg actgacgaat ctcttccggc agatcatcca aatctttttc
     6121 attacgcttc ggcaagatga tcgtgcgcag tcccaatcga tgcgccgcca gaattttatc
     6181 cttgatcccg ccgacgggca acacctggcc gcgcaaagtg atttcacctg tcatcgccaa
     6241 atcatcacgt accgatcggc ctgtcaacaa agacgcgagc gcggtcgcca tcgtcacgcc
     6301 cgcagacgga ccatctttcg gcacggcgcc ggccggtacg tgtagatgaa tatcatgcgt
     6361 ggcaaagaat ttatcggaca cacccaatgt ttccgcgtgt gatcgaacga aggacatggc
     6421 agcctgcgcg ctctcgcgca tcacatcacc caactgcccg gtcaattgaa agccgcgcgc
     6481 gccagacatt tgcgtcgctt cgatgaagat aatgtcgcca cccaccggcg tccacaccaa
     6541 gcccgtcgcc acgcccggca gatgcgtgcg ctcggcgatc tcgccataga aatgcggctt
     6601 gccaatatac ttgaccacgt ccggtgtatc cacctgcatt tcagtcgaga caccttctgc
     6661 aatttgcgtc gccaacttgc ggcatacacg gccaagctcg cgctcaagat tgcgcacacc
     6721 ggcttcgcgc gtgtaatcat ggatgatttg aaagatcgcg gcatcggtaa aagacacttc
     6781 gcttttacgc agaccgttct ctttgatctg gcgcggaact aaataaccct gcgcgatttt
     6841 gattttctct tgatcggtat aacccgatag ctggataact tccatccgat cgcgcagcgg
     6901 gccgggaata ttttccaaat aattggccgt gcagataaag atcacttgcg acagatcgaa
     6961 cggcaaatcc atataatgat cgcggaattc ccggttctgt tccggatcga gcacttccaa
     7021 caaggccgaa gacggatcgc cgcgagaatc cgaactcaat ttatcgacct cgtccagcat
     7081 gaagatcgga ttgcgtgttt cgattcgcct caatgcttga atgacgcggc cgggcagcgc
     7141 gccgatatac gtccgacgga agccgcgtat ctcggcttca tcccgcacgc cgcccaacga
     7201 ttgccggaca aacttgcggc ccatcgcccg cgcgatcgat tggccgagcg aggtcttacc
     7261 cacaccgggc ggaccgacga agcacaagat cacgccctcg cgttcgcggc gaatcatgtc
     7321 tttagtttcg cccgccgacg tgtcatcttc aacattcgcc cgttccgatt ttaattttcg
     7381 gacggcgaga tactctaaga tgcgctcttt gatatcctgc aagccatagt gatcttcatc
     7441 tagaatcttg cgcgcatgcg caatatccaa gttatccggt gtgatcttgc tccacggcag
     7501 attgaccagc caatccagat acgttcgaat gacaccatat tcagccgcgg ccgtcgggag
     7561 tctggagagc cgatcgagtt cacgcacgga ctctttatgc gcctcttcgg gcatggcggc
     7621 ttcttcgatc ttctggcgca gctcctcaac ttccatctgc tgctcgtcgt gctcgcccag
     7681 ttccttttgt atcgctttga gctgttcgcg caaataatat tcgcgctgaa ctttttccat
     7741 ttcggaatgg gcttcggtct gaatcttgtg gcccagttcc agcacttcta attccttgtt
     7801 cagcagtgcg acgatcttgc gcagcttggc agtgacatca tccagttcca ggatttcttg
     7861 cgcgtccgcc agttcaatgc gcatgtaagt ggcaatggca taggccagct gacgcgggtc
     7921 ctcaatgttg atcgccacag ccgccacttc atcgggaatg cctggcacca gttcaaccag
     7981 gcggccaaac aacgagccga tgttgcgccg cagcgcttca acctcgacgc tcttctctga
     8041 ttgttcaggc gcagattcaa ctctggcttt gagataaggt tcagtaccgg taaattcttt
     8101 gatgcgaatg cgctcgaggc caagcaccaa caatcgaatc gtgccatcag gcaggcgcag
     8161 caagcgctgc accgtggccg tggtgccaat ggtgtaacac tggtccggac ccggttcctc
     8221 gatcgacgga tctttgctgg ccaccagacc gatgactttc ttctgcagca tcgcgtcgtc
     8281 gatcagctgg actgatcgag cctggccgac ggtcaacggc acagcggtca atgggtagac
     8341 cactacgccg cgcagtggca gaatgggcag gtcgtcgggc agtgtgctgc cgatcgattt
     8401 atccggcgag atcgcgtcgg cgtccgcgtc agctagcagc gcaatgtcga gcgtttcagg
     8461 ccgaaatatt tgattgccta atgaagcgtt atattcatcg gtcatggggt ctagatcgca
     8521 gagctcaggc aactttgcct attgcccgag ttgaaaaata agaaggcgga attaaagatt
     8581 gcgcaggccc agatcaacca cttggacgcg gcgcgagcct gctttgggca aatgtacttc
     8641 taagaaaccg ttgtgataag tcgccaagat accggcctca tccacaatcc aatgcaagta
     8701 aacttcacag cggaactcgc cgtatcgaac ttccaattga tgataggcca ctttgggact
     8761 cggatccgat cgtctgcccg taacgatcaa gagttgatcc tgcacgaaca ctttaaaatc
     8821 ggtttcgtgc atgccggcaa tttcaatccg cacgacgacc gcgtcatcga tctcatacac
     8881 atcggtgggt ggctgccagg catgcgtatg ctcccagacg atccaactac cggaacccaa
     8941 ttgatgatga tgccgccgct caagatcgga ctgagatcga tcggctggct cacgcggctc
     9001 ggaataagac attgacatcg gcaaccacct gctcatggcg cgctgcgcgc aaaacatcga
     9061 caaatctttg accagcacga ttctaccata aaacggagca atagcgtatc gtaaatcgca
     9121 tatcgcacag cataattcga agtggcgata cgctaaaatc ctgcaacact tttgaggaag
     9181 tgaatgagat ggactctcaa caccatattg agttcgtgca ggccagtgtg ttcgttgcag
     9241 ccggtacaca gcttgtcggg caagttacga tcggtgcgaa tagcagcgcg tggttcaatg
     9301 cggtgcttcg tgggaaaaac caaacagatt gcgatcggcg aacggacgaa cgtccaagac
     9361 ggcgcgatca ttcacgtcga tacgtgccgc ctgtacctga tcagttccag cgtcacgatt
     9421 gggcacgct
//
LOCUS       DASRTJ010000350         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_58914,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000350 DASRTJ010000000
VERSION     DASRTJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58914"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VFF70_07140"
     CDS             <1..1529
                     /locus_tag="VFF70_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44507.1"
                     /translation="FDWSQLQGSGPNDWNQFQVQDNWIDDAAQHGRQVVGLMENTPHW
                     ATDGTTGSGVPRGLYLPIDNPNNLWAGFVRALVKRTVGRVDHWIIWNEPDIEPPDSGI
                     QFDGSVADYYQLVKVAYQVAKQENPSAIIHLAGLTYYHDTTYHRVAYLQRFVTEAKKD
                     AGAAANHDYFDVASLHIYHNSDEIYNVASIMRNILRQNGLKQPLWINETNVQPSSDPF
                     LPWPYPTWVVSLDQQADFLIHAFAMGLAAGADRIAVYKLIDIPAPQPGWPPDGLIRTD
                     RSHRPAYDALKVITTYFRDTRSARLVRTGSTDIVTLNRGSQTTRVAWSRSSAATTISL
                     PAFAQQATLVSVDGSTQPLSAINNRYRLTLPGAKCDDPKFGCAVGGSPIILVEDAPSK
                     SGGLVIVSTVTPASPCTNCTPTVTSAHTATPCPDCTPTPTRTRQPTKTFTPTLTTSPQ
                     PTSTSTASPTFTFTPTPTGLPSPTSTPLPSIANADLPIGLFAVGILLALILIVFLRKR
                     "
     gene            complement(1709..1846)
                     /locus_tag="VFF70_07145"
     CDS             complement(1709..1846)
                     /locus_tag="VFF70_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44508.1"
                     /translation="MSALNLKPGKHIGAILEAIREAQAEGEVQTKEEALQVARRMLEE
                     K"
BASE COUNT          420 a          570 c          455 g          405 t
ORIGIN      
        1 ttttcgattg gtcacaactg caaggcagcg ggccgaacga ctggaatcaa tttcaagtac
       61 aggataattg gatcgatgat gccgcgcagc atggccggca agtcgtcggc ttgatggaga
      121 acacgccgca ctgggcgacc gatggcacga caggcagcgg tgtgccgcgc ggactgtatc
      181 tgccgatcga taatccgaat aatctgtggg ccggtttcgt gcgcgcgctg gttaaacgca
      241 ccgtcggccg cgtcgatcac tggatcattt ggaatgaacc cgacatcgaa cccccggaca
      301 gcggcattca attcgatggc agcgtggccg attattatca attagtgaaa gtcgcttatc
      361 aagtggcgaa gcaggaaaat cccagcgcga tcattcatct ggccgggctg acgtattacc
      421 acgacaccac ctatcatcgc gtcgcgtatc tgcagcgctt cgtcacagaa gcgaaaaaag
      481 atgccggcgc cgcggcgaat cacgactact tcgatgtcgc ctcgctgcac atctatcaca
      541 actcagatga aatctacaac gtcgcatcga tcatgcgcaa tatcctgcgg cagaatggtt
      601 tgaagcaacc gctgtggatt aatgaaacga atgtccaacc gtcgagcgat ccgtttttgc
      661 cgtggccgta tcccacgtgg gtcgtgtcgc tcgatcaaca agccgacttt ctgattcatg
      721 ccttcgcgat gggcttggcc gccggagccg atcggattgc ggtgtataaa ctgatcgata
      781 ttcccgcgcc ccagcccggc tggccgcccg atgggctgat ccgcactgat cgatcgcatc
      841 ggccggccta tgacgcgctg aaagtaatca cgacttattt tcgggatact cgctcagccc
      901 gattggttcg taccggctcg acggacattg tcacgctcaa tcgcggctcg cagacgacgc
      961 gcgtggcgtg gtcgcgcagt tcagccgcca ccacgatctc attaccggcc ttcgcccaac
     1021 aagccacttt agtttccgtg gacggatcga cacagcccct ctccgcgatc aacaatcgat
     1081 atcgattgac gctgcccggc gccaagtgtg atgatccgaa attcggctgc gccgtgggcg
     1141 gatcgccgat cattctcgtc gaagatgcac caagtaaatc tggcggatta gttattgtca
     1201 gcaccgtcac acctgcgtcg ccctgcacga actgcacacc gacagttaca tccgcacaca
     1261 ctgcaacacc gtgtcccgat tgcacaccga cgcccacacg cacgcggcaa ccgacgaaaa
     1321 cgttcacgcc aacactaact acatcgcctc aacccacatc gacttctaca gcttcaccca
     1381 cgttcacctt cacgccgaca ccaaccggct tgccatcgcc aacatcgaca ccgcttccat
     1441 ccatcgcaaa cgccgatcta ccgatcggtc tgttcgcagt tggcattcta ttggcgttga
     1501 ttctgatcgt tttcctgcgc aaacgttagc tgtcatttca agcgagtgaa gcgaacgaga
     1561 aatctctatg ccagttagaa aaacactcgg cagaaataga gatttctcgc tgcgctcgaa
     1621 atgacaagct attctggggg attctgaacg gacaatactt cgtcatcact accgcgcgat
     1681 tatttgcgaa tcaatcacgc tacacgcatc acttttcttc cagcatgcgt ctcgcaactt
     1741 gtaacgcttc ctctttcgtt tgcacttccc cttcggcctg ggcctcgcgg atcgcttcca
     1801 agatcgcgcc gatgtgtttg cccggcttca aattcaacgc cgacatgaca
//
LOCUS       DASRTJ010000351         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_59205,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000351 DASRTJ010000000
VERSION     DASRTJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59205"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFF70_07150"
     CDS             complement(<1..600)
                     /locus_tag="VFF70_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZY44509.1"
                     /translation="MGYVESLLGKNEKILRMERQHWTTLFITLVVNLFLAAVLIAIAV
                     VLGNQDFTKDSNSVLRFAPLIFVVALIYPLGRLGWDYLQWSAEQYLVTSHRVIQTEGL
                     INKRTKDSSLEKVNDIQLTQSFFGRLLGYGELEIVTGSDEGTNILHRLNNPVAFKTAL
                     LDQKVALGQNAGGDQVRPGGAAGTAGGDDSLKRLGDLDDL"
     gene            complement(625..1551)
                     /locus_tag="VFF70_07155"
     CDS             complement(625..1551)
                     /locus_tag="VFF70_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZY44510.1"
                     /translation="MNQFLAALVEGDGYARNTISAYRNDLTQLIEYVAQQQPTIDRWS
                     VITSDILTNFTEHLRTMNVSKRGGGEAKPAAASTIARKVAALKSFFGYLVKANLASID
                     ASISLEAPRVAKRSPKTLSNEDMARLLAAPGTGNSPKALRDRSLLELLYATGMRVSEL
                     VSLQIDDLDLSARSVRVHSGESNKERQVPFDEHTAQALGVYLDRGRPALLKEAGDRAA
                     LFLNQRGQKLTRQGMWLIIKEYAARAGIAYVTPHVLRHSFAAHMLESNRASLSEIQHF
                     LGHANISTTQIYKQSPRSAGAVDEDVTRQSAE"
     gene            complement(1691..>2606)
                     /gene="queA"
                     /locus_tag="VFF70_07160"
     CDS             complement(1691..>2606)
                     /gene="queA"
                     /locus_tag="VFF70_07160"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZY44511.1"
                     /translation="DRSSGSIGHRHFYELPDLLKPGDLLVANESRVLPARIFARKAHT
                     GGAIEVLLLRREDDLTWQALLRGHRLRVGTRLEVETGFNAQVIEHSPTGEFVIRFDRA
                     IDAQLDRVGVMPLPPYIHARLDDPNRYQTVYAHTPGSAAAPTAGLHFTPQLIDRLKER
                     GIDLAYVTLHIGLDTFKPVESEDLEQHHIHTEYMDFDQATALQVRQAKQAGRRVIAVG
                     TTAVRVLETAARNGLQAFSGDTKLFIFPGFEFRAIDGLITNFHLPRSSLLMLVSAYAG
                     KDNIDRAYAEAITQKYRFFSFGDAMLIG"
BASE COUNT          636 a          723 c          663 g          584 t
ORIGIN      
        1 cagatcatct aaatcgccta aacgcttaag cgagtcatcg ccaccggctg tgccggccgc
       61 accgccgggt cggacttgat ccccgccagc attctgaccg agcgcgactt tttgatcgag
      121 cagcgcggtc ttaaaagcca ccggattatt gagccggtgc agaatattcg tgccctcatc
      181 gctgccggtc acaatctcca attcgccgta gcccaacaac cggccgaaga acgattgcgt
      241 taactgaatg tcgttcacct tctccaatga agagtctttg gtccgcttgt ttatcaggcc
      301 ctcagtctgg atgacacggt gtgaggtgac caaatattgc tccgccgacc attgcagata
      361 gtcccagccc aatcggccga ggggatagat taatgccacc acaaaaatca gcggcgcaaa
      421 tcgcaggacg gagttcgaat ctttcgtaaa atcttggttg cccaatacca cggcgattgc
      481 aatcaatacg gccgccagaa agagattaac aaccagcgta atgaacagtg ttgtccaatg
      541 ttggcgttcc atacggagga ttttttcgtt cttgcctaac agactttcaa cgtagcccat
      601 ggtttacctc acgatcgaaa gattttattc tgcggactgc ctagtaacgt cttcgtcgac
      661 cgcgcccgca gatcgcggag attgcttgta aatctgggtc gtcgatatat tcgcgtgacc
      721 cagaaagtgc tgaatctcgg acaggctggc ccggttgctt tcaagcatgt gcgcagcaaa
      781 cgaatgacgc agcacatgcg gtgtcacata cgcaatgccg gcgcgcgctg catattcttt
      841 gataatcagc cacatgcctt gccgggtgag tttttggccg cgttgattca agaagagcgc
      901 cgcacgatcg cccgcctctt tcaagagagc tggccgaccg cgatcgaggt acacgcccag
      961 cgcttgggcg gtgtgttcat cgaacggcac ctgccgctct ttgttgctct cgccgctatg
     1021 cacgcgcacc gatcgagccg ataaatcaag atcgtcgatc tgcagtgaaa ccaattcaga
     1081 cacgcgcatg cctgtggcat agagcaattc cagcaacgat cgatcacgca gtgccttcgg
     1141 cgagttgcca gtcccgggcg cggccagcaa gcgggccata tcttcattcg ataaagtttt
     1201 cggcgagcgc ttggccacgc gcggcgcttc aagactgatc gaagcatcaa ttgaagccag
     1261 attcgctttg actaaataac caaagaaaga tttcaaggcc gccaccttac gggcgatcgt
     1321 tgaagccgca gccggcttgg cctcgccgcc gccgcgcttg gaaacattca ttgtgcgcag
     1381 gtgttcggtg aagttcgtta aaatatccga cgtgatcacc gaccaccgat caatggtagg
     1441 ctgctgctgc gccacgtact caattaattg agtcaagtca ttgcgataag ccgagatagt
     1501 attgcgcgca tagccatccc cttcaaccag cgcggccaaa aattgattca taaccgatgt
     1561 gagttgctca gtatttgttg agggtgtcat aaattcagcc ttgtggtgaa ttgcccgcaa
     1621 tcaggcaagg attgatcact attatatcaa gttgccggtc aaaatcaaga aaggattatg
     1681 gcgaggatac tcacccgatc agcattgcat caccgaaact gaagaaccgg tatttctgcg
     1741 taatggcttc ggcataagct cgatcgatgt tgtcttttcc agcataagca gacaccaaca
     1801 taagtaacga cgatcgcggc aaatgaaagt ttgtgattaa gccatcgatc gcgcggaact
     1861 caaagcccgg aaaaataaac agcttggtgt cgccgctaaa ggcttgcaaa ccattccgcg
     1921 ccgcggtttc caaaactcga accgccgttg tgcccactgc gatcactcgc cggcctgctt
     1981 gtttagcctg acgaacttga agcgcagtgg cttgatcgaa atccatatac tcggtgtgaa
     2041 tatgatgctg ctctaaatct tccgactcga cgggcttgaa tgtatccaaa ccaatgtgca
     2101 aagtgacata tgccagatcg atgccacgct ctttcaatcg atcgatcaac tgcggcgtga
     2161 agtgcaggcc agccgtcggc gcggccgccg atcccggcgt atgagcatat accgtctgat
     2221 agcgatttgg atcatcaagc cgtgcatgaa tgtatggcgg taacggcatc acgcccactc
     2281 gatcgagttg ggcatcgatc gcgcgatcga accgtatcac aaattcgccc gttggtgaat
     2341 gctcgatcac ttgagcgtta aaaccggttt caacttccag ccgggtacca acccgtaagc
     2401 gatgcccacg caaaagagct tgccaagtaa gatcgtcctc tcggcgtagc agcaaaacct
     2461 cgatcgcgcc gccagtgtgt gccttgcgcg caaatattcg cgccgggagc actcgactct
     2521 cattcgccac caacagatcg ccgggcttga gcagatcggg caattcataa aagtggcgat
     2581 gcccgatcga gccggaagat cgatcg
//
LOCUS       DASRTJ010000352         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_21314,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000352 DASRTJ010000000
VERSION     DASRTJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21314"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFF70_07165"
     CDS             complement(<1..307)
                     /locus_tag="VFF70_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44512.1"
                     /translation="MTTSDQAHTLRLTHVEDEPIIFYGPPDRLQGTIRLHNTSTEKLK
                     LSSVALDAPHMLGPGRVPLRQLQLAARLSPDEQAKVPATIQLDSSTPPGKYEAVMLLG
                     "
     gene            complement(316..711)
                     /locus_tag="VFF70_07170"
     CDS             complement(316..711)
                     /locus_tag="VFF70_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44513.1"
                     /translation="MSKGGGITIPDNYTIKLGSDGSTIKVDADLDNIHVKELPRIELG
                     DINVHVKEFPTITLNTNVAVTELPEIHAKTDSNVNIAIKEIPDTRVHLPANFNVGLSV
                     LGYQLFNISLCGEAQVISEKYAPRRMEIC"
     gene            complement(1049..1714)
                     /locus_tag="VFF70_07175"
     CDS             complement(1049..1714)
                     /locus_tag="VFF70_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HZY44514.1"
                     /translation="MLAKVYSCAVIGLEGQVVEVEVDHVNRGLPSFMLVGLPDAAIKE
                     SSERVRAAIKNSGLMFPYHRLTVNLAPADLRKVGPGYDLPIAVGVLAVSDQIPIEALD
                     RSMIVGELSLDGSVRHVRGVLPIAALARANGFKTLFVPQVDAAEAALIPELKVIPVSN
                     LADLVNHLHGVALIEVQVAVIPEASDFALSGYTNFAEIRGQEHVKRAVEVAAAGGHNV
                     VIL"
BASE COUNT          405 a          468 c          508 g          435 t
ORIGIN      
        1 cgcccaatag cataacggct tcatacttgc cgggcggtgt gctcgaatcc aattggattg
       61 tcgcgggaac tttcgcttgc tcatcgggcg acaaacgcgc cgcgagctgc aattggcgca
      121 gcggcacacg gcctggtccc agcatatgag gtgcatcgag tgccacgctc gacaatttta
      181 gtttttcggt cgacgtgttg tgcaagcgga tggtgccctg caaccgatcg ggggggccgt
      241 aaaaaatgat cggctcgtct tccacgtgcg tgagccgcag agtgtgcgcc tggtccgagg
      301 tggtcatgcg acctcctaac aaatctccat gcggcgcggt gcatactttt cagaaatgac
      361 ttgcgcttcg ccgcacagtg agatgttgaa caattggtaa cctagcaccg acaaaccaac
      421 attgaagttg gctggcaggt ggacgcgggt gtcgggaatt tctttaattg cgatgttgac
      481 gttggaatcg gtcttggcgt ggatttcggg gagttccgtg acggcgacgt tagtgttcaa
      541 agtgatcgtg gggaattctt ttacatgtac gttgatgtcg ccgagttcga tgcgcggcag
      601 ctccttgacg tgtatgttat cgagatcggc atctactttg atcgtcgatc catctgaacc
      661 cagcttaatg gtgtagttat cgggaatagt tatgccaccg cccttactca tgagagcctc
      721 cttgccggtt attcagttgt gaagtcagag ttgcacgatg agttatagta atcgagatgg
      781 catgtttcga tccgataata gtatcgcata atctgagcga gatgtcaatc acctgtacac
      841 gtcgtcaggg ctctaacaaa gtcgagattg tcatgctgag cgcagcgaag catctctatg
      901 tcaatcgggc gagacccttc gctatgctca gggtgacagt gctcaagcgt caagaacacg
      961 atcgcgccaa attgtcaatg gactttgtta agaccctgtc ggatgaatac gttacgagag
     1021 ggatagctta aacgtgatcg tttttcgttc acaatatgac cacattgtgt ccgcccgccg
     1081 ccgcaacttc gacggcgcgc ttcacatgtt cttggccgcg aatctcggcg aagttggtgt
     1141 acccacttaa tgcaaaatca gatgcttcgg gaatgactgc gacttgcact tcgatcagcg
     1201 cgacgccgtg cagatgattc accaagtcgg ccaagtttga aacggggatc accttcagtt
     1261 cggggatcaa ggcggcttcg gccgcatcta cttgtggaac gaacagcgtc ttgaagccgt
     1321 tcgctcgggc cagagccgcg atcgggagca caccgcgcac atgccgcacc gatccatcga
     1381 ggctcaattc gccaacgatc atcgatcgat cgagtgcctc gatcggaatt tgatcggaca
     1441 ccgcgagcac gccgacggcg atcgggagat cgtagccggg gcccactttg cggagatcag
     1501 ccggcgcgag gttaaccgtc aagcgatgat acggaaacat caggccggaa tttttaatcg
     1561 cggcgcgcac gcgctcgctc gattctttga tcgccgcatc gggcaggccg accagcataa
     1621 aagacggcag gccgcgattg acatgatcga cttcgacctc gacgacctgg ccttccaatc
     1681 cgatcacggc gcacgaataa actttggcga gcatactgtt ccttttcctc ccggaggttc
     1741 caaacctccg ggaggatatt ggctgagtgt agcatagaga tcaatttgat tcaaatatgt
     1801 ttttgatgtc ttatca
//
LOCUS       DASRTJ010000353        11857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_59405,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000353 DASRTJ010000000
VERSION     DASRTJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59405"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFF70_07180"
     CDS             complement(<1..721)
                     /locus_tag="VFF70_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZY44515.1"
                     /translation="MTTSSNPKIQQLRDLREKAKQGGGAEAIEKQHSKGRLTARERID
                     LLLDSGSFRELDMFVTHRATDFGMEDKKYLGDSVVTGWGTIDGRLVYVYSQDFTVFGG
                     SLSQVHAQKILKVMDQAMKNGAPMIGLNDSGGARIQEGVVALAGYSDIFLRNTMASGV
                     IPQISAIMGPCAGGAVYSPALTDFIFMARDTSYMFVTGPDVVKAVTHEEVTQEELGGA
                     SVHSEKSGVCHVAADNEADTLY"
     gene            complement(718..1137)
                     /locus_tag="VFF70_07185"
     CDS             complement(718..1137)
                     /locus_tag="VFF70_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44516.1"
                     /translation="MKRPFSVTLLALAVLLFAIYNAVRTAIAIYALNQLDFLTDLDIR
                     TPVIVLMVIGIDWAIGFTLAAIGLWRLKAWGRQWMIIALIAYEILIWIERLTLEQSPY
                     EQLTRPGDVAITLLVAVSTVGFFFMPRIRRVFQTEQP"
     gene            complement(1146..2312)
                     /locus_tag="VFF70_07190"
     CDS             complement(1146..2312)
                     /locus_tag="VFF70_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZY44517.1"
                     /translation="MTGPNLDLTEEQKMIRDAARDFAQNEIAPIAAHHDETGEFPLET
                     IRKMGALGLMGVEVPEEYGGAGMDTLAYVLAIEEVAKADVSHSTIMSVNNSLYCHGIL
                     KFGTEEQKKKYVTPIASGQKIGAYSLTEPMSGSDAGAMKSRAVRDGDHYLINGRKSWV
                     TSGPVAAYMVLFTMTQPEKGHNGITAFIIETDKPGFVKGKTEPKLGIRASATCEIVYD
                     NYHVPVENRLGEEGQGFKIAMTVLDAGRIGIASQALGVAEAAFDAAVEYTKTREAFGQ
                     AIGQFQGIQWMLSDMKTRIEAARMLIWSAALAKERSKKDGGRYTLEASMAKLFASETA
                     MWVTEKALQIHGGMGYSKEMPLERYFRDAKITEIYEGTSEIQRLVIARNVLGLK"
     gene            2428..3987
                     /gene="ggt"
                     /locus_tag="VFF70_07195"
     CDS             2428..3987
                     /gene="ggt"
                     /locus_tag="VFF70_07195"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZY44518.1"
                     /translation="MLNFPSRRPVVTSKNGMVATSQPLAARVGLKILMDGGNAIDAAV
                     AMAAMLNVVEPMSTGVGGDAFALTYNAKTDQLTALNASGRAPNAISIDAVKRLGHTEM
                     PETGPLSITVPGTVDGWEKILQQHGSMLLSEVLKPAIQYAEQGFPVTPIIANEWHEHE
                     EILHSTPYLINGHAPRAGQIFKNPDLAQTLRIIAEGGSDAFYRGSIAKQIAQHVQSQG
                     GYLSATDFAQHTSTWDIPVSTVYRDAQIFECPPNGQGIIALMALNILEGFDLQAMGYG
                     SVAYYHHVIEALKLAFADGFAHIADPNRVAVPIEKMLDKEYAASRRKLINPNHASRNT
                     TTGLPRGGTVYLTSIDKDRNACSFINSIYYGFGSGVMVPGTGIVLQNRGTLFSLDEAH
                     PNSLAPGKRPFHTIIPAMAFKNGKLWSSFGVMGGHIQPQGHVQVMLNSIDFKLNPQRA
                     IDAPRVVWYEGNQIDVESPIESAPLQSLGHEIISARGRYGGGQMIVIDQDNGALLGGS
                     DPRKDGCAIGY"
     gene            4032..4232
                     /locus_tag="VFF70_07200"
     CDS             4032..4232
                     /locus_tag="VFF70_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZY44519.1"
                     /translation="MDEALIPLTTEPLEEGGYLATSPVWDELLAQGRTIAETMEIAQD
                     VARKLVESHIEHGDSLPSRIDR"
     gene            complement(4333..6387)
                     /gene="tkt"
                     /locus_tag="VFF70_07205"
     CDS             complement(4333..6387)
                     /gene="tkt"
                     /locus_tag="VFF70_07205"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZY44520.1"
                     /translation="MSSSSNLDQLSINTIRFLSVDAIQKANSGHPGLPMGAAPMAYVL
                     WTRFMHHNPIDPKWLNRDRFVLSAGHGSMLLYSLLHLTGYDLSMDQIQNFRQWGSQTP
                     GHPEHGLTPGVETTTGPLGQGFANGVGLALAEAFLAAKYNRPGHDIVNHTTYGIVSDG
                     DLMEGISHEAASLAGDWQLGKLIYLYDDNSITLAGSTSITYTEDRAKRFEAYGWHVQI
                     VSDGNDLKSIDKAVLAARTVSDRPSIIMVRTIIGFGAPHKQNTFDAHGSPLGLDEVKA
                     AKENLGWPIDPPFYLPGEALEHFRGAIDRGKTDQAQWQKNFEAYQKEFPELAKELIQA
                     TNSELPNGWNADLPVFTADKPLATRASSGQVLNAIAKKIPVLIGGSADLNPSTDTALK
                     GFGDFQSPAFNSAGSQGLVGGGTSYAGRNIAYGVREHAMGAIASGLALHGGVIPFTAT
                     FMQFSDYMRPAIRLAALMEQRVIYVFTHDSVFLGEDGPTHQPVEQLAALRAIPNLHVI
                     RPADANEVSIAWQLAIERRAGPTALIFTRQAVPTFDRSKMNSADGVRRGGYVLIDSDG
                     SPDIILIATGSEVQLAVGAREELLKQNVKARVVSLPCWELFDAQPRAYRESVLPKNIT
                     KRLAIEAGVAQGWQRYVGDHGEVLSIDRFGASAPAKVIAEKFGFTIENVVKRAKSLIT
                     DL"
     gene            6589..7137
                     /locus_tag="VFF70_07210"
     CDS             6589..7137
                     /locus_tag="VFF70_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZY44521.1"
                     /translation="MNLFDLFLPFLLIGGILLCFRRAFLRTLVSIFTLLIALVVSALL
                     YTPLLTWFSGTTGSGGTPSQNGGSIVFTGLLILFFVLLEFAVDHNYPNMRIAWLKNWD
                     HILGGVIGVFWTALAISLILLILHYGSLTVGASQVSGIANSIEQSKVANVFRDFFSIP
                     LSFIKLMFPNGLPEILKYFGRY"
     gene            7270..9660
                     /locus_tag="VFF70_07215"
     CDS             7270..9660
                     /locus_tag="VFF70_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZY44522.1"
                     /translation="MPTVVFGTGWGMEAQHWLTLELNKVLDRLARYTSFSAGSELAAQ
                     LVPSTFSDVVEQRLAETREARSILVTRSDLGLGGVHDIRSFAADATRGIMLLPIDLLA
                     VRSTLIASRDIRRTLIKAADQFPRLAAIAQRLFESSGIINEISRCIDENNSEVRDDAT
                     PELAAIRRELRDSSNRLQDKLRRMISSTTNAKYLQEAIITQRSGRFVIPVKAEYRSRV
                     PGIVHDQSASGATLFVEPLSTVELNNKLRELQLREEREVQRVLQMLSGLIAQEAQPIT
                     YAVEALAELDVILAKARYCDDLQAIEPALNTGRRSMARLIRARHPLLDPATVVPLDLE
                     LDPDLRVVVITGPNTGGKTVALKTVGLMILMTQCGLHIPASSGSEVSIFDQVYADIGD
                     EQSIEQSLSTFSSHVVNIVGFIDRVTAKSLVLLDELGAGTDPTEGSALARSILDHLSA
                     TDALTIVATHYPELKVWAHSTQGAINASVEFDPETLRPTYKLTVGLPGRSNAFAIAKR
                     LGLKDDIVSHARSMVAEDNLKAEDLLAEIHQQREATERSRASADRLRDEAEKLTRQLR
                     DRLSAIEQERASILEAAQSESQQLIDTFKDQIDQIKASLLTADQATILTQLDQLEAQA
                     ESIEPPGPIEKPAINPRHSIRLGDTVYVRKLNATGDVASIDRDTVEVIAGSLRMRLKP
                     DEIEWRSSPNTNVAAAHERPEAVEYANVRSTTAAHIEIDLRGLRADEAVTEAEKQIDA
                     ASLAGLPYVRIIHGKGTGALRKAIREALKNNPAIKNLENGKDEEGGDGVTIAHLKV"
     gene            9742..11337
                     /locus_tag="VFF70_07220"
     CDS             9742..11337
                     /locus_tag="VFF70_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZY44523.1"
                     /translation="MMEQFLATETLIIGLLLIVSLVAMAVRRLRVPYTVALVIVGLLI
                     TLQQSIQIELTPELILALFVPPLVFEAAFHLNFKRLRNNLIPILVLAVPGVLLTTILV
                     GLIVSAGVNLPLSTALVFGALISATDPVAVIALFRTLGTPHDLGVLVEGESLFNDGTA
                     IVIFNLMIAAAGAAVVNGAVQAATHFDLGRAIIDFVIISLGGLGIGVFLGWLVSRLIA
                     RLDDYLIETTLTTVLAFGAYLIAERLHVSGVLAVVGAGLINGNLGPRGMSPTTRIVLF
                     NFWEYLAFVTNSLVFLLIGLQVKLTQLIANLGPIAIAVLAVLISRAIVVYGLTWLINR
                     SKLKVSAAYQHVQFWGGLRGAISLALALSLPAALADRDLLRIMTFGVVLFTLVGQGTT
                     MQFLIKKLKLTERIEGKLEFERRHGRLLAARAERDRLERLHREGTLSESTWEQLAPDL
                     DQHIRSQLAAQRELLAQHPVLQTEELEDAKREGLRARRATLSTLLRDGVISERAYEEL
                     VAEIDAKIENQTEPDSDEAQPAD"
     gene            11351..>11857
                     /locus_tag="VFF70_07225"
     CDS             11351..>11857
                     /locus_tag="VFF70_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZY44524.1"
                     /translation="MRPSSLDAISDHLIFLYGLRAGRVAADRIIRRIDQYRAIITPRP
                     SRNTFSQHDAMLITYGDQLRASNESPLHTLTDFCQKHLSGLLNGIHLLPFFPYSSDDG
                     FSVIDYRQVDPRLGAWDDIDRLSQHFRLMFDAVINHISAESEWFKKFLQDDPKYQDYF
                     IVVDGNPDL"
BASE COUNT         2618 a         3344 c         3169 g         2726 t
ORIGIN      
        1 ggtataacgt atccgcctcg ttatctgcag caacatggca cacacctgat ttttcggagt
       61 gtacacttgc accgccgagt tcttcctgtg ttacctcttc gtgtgttacg gcctttacga
      121 catcgggccc tgtgacaaac atataagaag tatcgcgcgc catgaaaata aaatctgtaa
      181 gtgcgggaga ataaactgcg ccgcctgcgc atggtcccat gattgctgaa atttgcggga
      241 taaccccgga agccattgta ttacgcagaa agatgtccga ataacccgca agagccacca
      301 cgccttcttg aatgcgtgcg ccgcccgaat cgttcaagcc gatcatcggc gcgccgttct
      361 tcatggcctg atccatcacc ttcaaaattt tttgcgcgtg gacttggctc aaactgcccc
      421 cgaacaccgt gaaatcctgc gagtacacat acaccaagcg tccatcgatc gtgccccagc
      481 cggtcacgac gctgtcgccc aaatattttt tgtcttccat gccgaagtcg gtggcgcggt
      541 gtgtgacgaa catatcgagt tcgcggaacg aaccgctgtc caacagcaga tcgatccgtt
      601 cgcgcgcggt caggcggcct ttgctgtgct gcttctcgat cgcttccgca ccgccgccct
      661 gtttggcctt ctcgcgcaga tcacgcagtt gttgaatttt tggattgctg gatgtcgtca
      721 tggttgctcc gtctgaaata ctcgtcgaat tctcggcatg aaaaagaaac cgaccgtgct
      781 gaccgcgacg aggagggtaa tcgcgacatc gccgggccgg gtgagttgtt cgtagggcga
      841 ttgttccagt gtcaagcgtt cgatccagat tagaatctcg taagcgataa gggcgatgat
      901 catccactgc cgcccccacg ctttaagccg ccacaaaccg atcgcggcca gtgtgaaccc
      961 gatcgcccaa tcgatcccaa tgaccatgag gacgatcact ggcgtgcgaa tatcgaggtc
     1021 ggttaagaag tccagctgat tgagtgcgta aatagcgatc gctgtccgta cagcgttgta
     1081 gatcgcaaat aataacactg ccagcgcaag taaagttacg gagaatggtc gtttcacctg
     1141 gctcactact ttaatcccaa cacattgcgc gcgatgacca gccgctgaat ctcgctggtg
     1201 ccttcgtaaa tctcggtgat cttggcatcg cgaaaatagc gttcgagcgg catctctttg
     1261 ctgtagccca taccgccgtg aatttgcagc gctttctcgg taacccacat cgccgtctcg
     1321 ctggcaaata attttgccat gctcgcttcg agggtatagc gtccgccgtc tttcttcgat
     1381 cgttctttgg cgagtgccgc actccagatc aacatgcgcg ccgcctcgat gcgcgtcttc
     1441 atgtctgaca gcatccattg aatcccctga aactgcccga tcgcttggcc gaacgcttcg
     1501 cgcgttttag tatattccac ggccgcatcg aaggcggcct cggccacacc caaggcttga
     1561 ctcgcaatgc cgatgcgtcc tgcatccagc acggtcattg cgatcttgaa gccctgccct
     1621 tcttcaccca atcggttttc aaccggcaca tgatagttgt cgtacacaat ctcgcacgtc
     1681 gccgacgcgc gaatgcccaa cttcggctcg gtcttgcctt tgacgaaccc gggcttatcc
     1741 gtctcaatga taaaggccgt gataccgttg tgtccttttt cgggttgtgt catggtgaac
     1801 aacaccatgt aagcggcgac ggggccagag gtcacccaac tcttgcgacc gttgatcagg
     1861 taatgatcgc cgtcgcgcac ggcgcggctc ttcatcgcgc ccgcgtccga gccgctcatc
     1921 ggctcagtca gtgaatacgc gccgatcttc tggccggacg cgattggtgt cacatatttt
     1981 ttcttctgct cctccgtgcc gaatttcaaa atgccgtggc agtacaatga attgttgact
     2041 gacatgatcg tgctgtggct gacatcggcc ttggcgactt cttctatcgc cagcacatac
     2101 gccagcgtat ccatgcccgc gccgccatat tcttccggca cctcgacgcc catcaagccc
     2161 agtgcgccca tcttcctgat cgtctccagc ggaaattcgc cagtctcgtc gtggtgcgcg
     2221 gcgatgggcg caatttcatt ctgcgcaaaa tcgcgcgccg catcgcggat cattttttgc
     2281 tcttcagtga gatcaagatt ggggccagtc atatggggtg ctcctctaaa aagtggtaga
     2341 ctgcattata acttgaaaag tgaagagtga cgagtaaatt acgcatcact cgtcactctt
     2401 cgctcgtcaa attctgaagg agtctcaatg ctaaattttc cttcccgtcg accggttgtc
     2461 acatccaaga acggtatggt cgcgacgagc cagccgctcg ccgcgcgagt gggcttaaag
     2521 attttgatgg atggcggcaa cgcaatcgat gcggccgtgg cgatggcggc gatgttgaac
     2581 gtggtcgaac cgatgtcgac gggcgtcggt ggcgatgcgt tcgccttgac gtacaacgct
     2641 aagaccgatc agctgacagc gctcaacgcg agcggccgcg cgccaaatgc gatctcgatc
     2701 gatgcagtga agcggttggg gcacactgaa atgccagaga ctgggccgtt atcgatcacg
     2761 gtgccgggta cggtcgatgg ttgggagaag attctgcaac aacatggatc gatgctgctg
     2821 agtgaagtgc tgaaacccgc gattcaatat gccgaacaag gttttcccgt aacaccgatc
     2881 atcgccaatg aatggcatga acatgaagag atattgcact cgaccccata cctcatcaac
     2941 ggccacgcac cacgcgcggg ccaaatcttc aaaaatcccg atcttgctca gacgttgcga
     3001 ataatcgccg aagggggcag cgatgcgttc tatcgcggat cgatcgctaa gcaaatcgcg
     3061 cagcatgttc aatcacaagg cggttactta agcgcaacag attttgctca acatacttct
     3121 acttgggata ttcccgtctc aacggtttat cgtgacgccc aaatatttga gtgtccgccc
     3181 aacggtcaag gcatcatcgc gctgatggcg ttgaatattt tggaaggatt tgatcttcaa
     3241 gcgatgggct acggatcagt cgcgtactac catcacgtga tcgaagcgtt gaaacttgct
     3301 ttcgcagatg gctttgccca catcgccgat ccgaatcgcg ttgccgtgcc gatcgagaaa
     3361 atgttggaca aagaatatgc ggcttcgcgc cgaaaactaa tcaatcccaa tcacgcgtcg
     3421 cgcaatacaa caaccggctt gccgcgcggc ggcacagtgt atctcacctc gatcgataaa
     3481 gatcgcaacg cctgctcgtt catcaactcg atctattacg gctttggatc gggcgtgatg
     3541 gtgccgggca cgggcatcgt gctgcaaaat cgaggcacgt tgttctcatt agatgaagcg
     3601 cacccgaatt cgctcgcacc gggtaagcga ccgttccaca cgatcattcc ggcaatggct
     3661 tttaaaaatg ggaagttatg gagtagcttt ggtgtaatgg gtggccacat tcaaccgcaa
     3721 ggccacgtgc aggtcatgct gaactcgatc gatttcaagc tgaacccgca gcgcgcgatc
     3781 gatgcaccgc gcgtggtgtg gtatgaaggc aatcagattg acgtagaatc gccgatcgaa
     3841 tctgcaccat tacaatcgtt gggacatgaa atcatttcag cgcgtggaag atacggcggc
     3901 ggacaaatga tcgtgatcga tcaagacaat ggcgcgttgt taggcggatc agatcccagg
     3961 aaggacggct gcgctattgg atattgaatt aaaagtataa ttgacttgca atgcaattca
     4021 ggcggtcatc tatggacgaa gctttaattc cactaacaac tgagccgctc gaagagggtg
     4081 gctatcttgc gaccagtcca gtctgggatg aattgctggc gcagggacgc accatcgccg
     4141 agacaatgga aattgcgcaa gatgtagcgc gcaaattggt cgaatcacac attgagcatg
     4201 gcgattcact gccaagccgg attgaccgtt gatgaattca acgcacttta gctgcaccgc
     4261 tattaaacaa aggcccgact ctaaaccgaa tcgggacttt tgattagcgt tacctcgatc
     4321 agaaatcaaa gatcaaagat cagtgatcag agattttgcg cgcttgacga cgttctcgat
     4381 ggtgaagcca aatttttcgg cgatgacttt ggccggggcg gatgcgccaa accgatcgat
     4441 actcagcacc tcaccgtgat cgcccacata cctctgccaa ccctgcgcga caccagcctc
     4501 gatcgccagc cgtttggtga tatttttcgg caacacgctt tctcgatagg ctcgcggctg
     4561 cgcatcgaac aattcccagc acggcaaact gacgacgcgc gctttcacat tttgcttcaa
     4621 caactcttcg cgcgcgccga ccgcgagttg aacttccgag ccggtcgcga tcaaaataat
     4681 atcaggcgat ccatcactat cgatcagtac gtagccgccg cgtcgcacgc cgtctgccga
     4741 attcatcttc gatcgatcga aagtgggcac agcctgccgc gtgaagatca aagccgttgg
     4801 gcccgccctt cgctcgatcg ccagctgcca cgcgatcgag acttcgttcg cgtcggcggg
     4861 tcgaatcaca tgcaagttcg gtatcgcgcg taacgccgcc aactgttcaa cgggttgatg
     4921 cgtcggccca tcttcgccga ggaagacact gtcatgcgtg aaaacgtaga tcacgcgttg
     4981 ttccatcagc gcggctaagc gaatagccgg gcgcatgtag tcgctgaatt gcatgaacgt
     5041 ggccgtgaat ggaatgaccc cgccatgcag cgccaggcca gaggcgatcg cacccattgc
     5101 atgttcgcgc acgccgtagg caatgttgcg tcctgcgtaa ctcgtgccgc cgccgactaa
     5161 gccttgcgaa cctgccgaat tgaatgcggg actttggaaa tcgccgaagc ctttcaacgc
     5221 cgtgtctgtc gatggattaa gatcagctga gccgccgatc aagacaggaa tctttttcgc
     5281 aatggcattc aagacttgac cggatgaggc gcgcgtcgcc agcggcttat cagccgtgaa
     5341 caccggcagg tcggcattcc agccattggg cagctcagaa ttcgtggctt gaataagttc
     5401 tttagcaagt tcaggaaact ctttttggta agcctcaaaa ttcttttgcc actgcgcttg
     5461 atccgttttg ccgcgatcga tcgcgccgcg aaaatgttcc agcgcttcgc ccggcagata
     5521 aaacggcgga tcgatcggcc agccgaggtt ttctttcgcg gccttcactt catcgaggcc
     5581 gagtggcgag ccgtgcgcgt caaacgtatt ctgcttgtgc ggagcgccga agccgatgat
     5641 cgtccgcacc atgatgatgg acggccgatc ggatacagta cgagccgcca acacagcttt
     5701 atcgatcgat ttcagatcat tcccatcgct gacgatttgc acatgccagc catacgcttc
     5761 aaagcgcttc gctcgatctt cggtatacgt gatcgaagtg gagcccgcca gcgtgatcga
     5821 attgtcatca tacaaataaa tcaatttgcc caactgccaa tcgccggcca gcgatgcggc
     5881 ttcatgcgag atgccttcca tcagatcgcc gtcgctgacg atgccgtatg tggtgtgatt
     5941 gacaatgtcg tggccgggcc ggttgtattt cgcggcgagg aaagcttcag ccagtgccag
     6001 acccacaccg ttagcaaagc cttgaccgag ggggccggtg gttgtttcaa cgccgggcgt
     6061 cagaccatgt tcgggatgcc ctggtgtttg actgccccac tgtcgaaagt tttgaatctg
     6121 atccattgat agatcataac cggtgagatg cagcagcgaa tatagcagca tcgagccatg
     6181 tccggcggat agcacaaacc gatcgcggtt cagccacttc ggatcgatcg ggttatggtg
     6241 catgaagcgc gtccacaaca cgtaggccat tggcgcggcg cccattggca aaccgggatg
     6301 gccgctgttc gctttttgaa tcgcatcgac ggacaggaat cgaatcgtat tgatgctgag
     6361 ttgatcgagg ttcgaagaag aggacatagt caccagcctt tcaataagca atggcctggg
     6421 gctgccattg ctacaattct cgtttggtga gatggcatcc ccgtgccatc tcttgattaa
     6481 ttatacgcgg aattgaaaat gtgcgacgcg cttttactgc ttgtgtgtca acattggcta
     6541 tgctatactc gccaacagac acatcggctg catatcgagg tgatgatcat gaacctgttc
     6601 gatttatttt tgccttttct gttgattggc ggcattctgc tgtgtttccg gcgcgccttt
     6661 ctgcgcacgc tggtttctat cttcaccttg ttgattgcgc tcgtcgtctc cgcgctgttg
     6721 tatacgccgc ttctcacctg gttcagcggt acgactggca gcggcggtac tcccagccaa
     6781 aacggcggat cgatcgtttt caccggcttg ctgatcttgt ttttcgttct attggaattc
     6841 gcggtcgatc acaattaccc taacatgcgc attgcctggc tgaagaactg ggatcatatt
     6901 ttaggcggag tgatcggtgt gttctggacc gcgctcgcta tcagtttgat tttgctgatc
     6961 ctgcactatg gcagtttgac ggtcggcgca tcgcaagttt ccggaatcgc taacagcatt
     7021 gagcaatcca aagtcgccaa cgtctttcgc gattttttta gcatcccgtt aagcttcatc
     7081 aaactgatgt tcccgaatgg cctaccggaa atactgaagt acttcgggcg ttactgatcg
     7141 cagctgggta tcaacagacg gggacggctc aacgccgtcc tctgcatttg tgatgcggcc
     7201 cctcgccaca cgacgtattg agcagatcat ccatcgaaac ggatcgcagt cgtgagggga
     7261 gggtcgaaga tgccgaccgt agttttcggc acagggtggg gcatggaagc acaacattgg
     7321 ctcacgcttg agctgaataa agttttagat cgattagccc gctacacgtc gttctccgcc
     7381 ggatcagaac tcgccgcgca attagtacct tctaccttta gcgatgtggt cgagcagcgt
     7441 ttagcggaaa cgcgcgaagc ccgatcgatc ctcgtcaccc gatcggatct cggcctcggc
     7501 ggcgtgcatg atattcgatc gttcgcggct gatgcgacgc gcgggatcat gctgctgccg
     7561 atcgatctgc tggccgtgcg atcaaccttg atcgcttcgc gcgatattcg ccgcaccttg
     7621 atcaaagcgg cggatcagtt tccccgcttg gcggccatcg ctcaacggct gtttgaatca
     7681 agcggcatta tcaatgaaat aagccgctgc atcgacgaga acaactcaga agtgcgcgac
     7741 gatgccacgc ccgaactggc cgcgatccgg cgcgaactgc gcgactcgtc gaatcgcctg
     7801 caggacaaat tgcggcgcat gatcagcagc acgaccaacg ccaaatattt gcaagaggcg
     7861 atcatcacgc agcgcagcgg gcgcttcgtc attcccgtca aagccgaata tcgcagccgc
     7921 gtgcccggca tcgtccacga tcaatcggcc agcggcgcga cgttgttcgt cgagccgtta
     7981 tcgacagtag aactgaacaa caaactgcgc gaactgcagc tgcgcgaaga gcgcgaagtt
     8041 caacgcgtgc tgcaaatgtt gtcggggtta atcgcgcaag aagcgcagcc gatcacgtac
     8101 gccgtcgaag cgttggccga gctcgatgtg attctggcga aggcgcgcta ctgcgatgat
     8161 ctgcaagcga tcgaacccgc tttgaacaca ggcagacgat cgatggcgcg tttgatccgc
     8221 gcgcggcatc cgctgcttga tccagccacc gttgtcccgc tcgatctcga acttgatccg
     8281 gacctgcgcg tggtcgtcat caccggcccg aacaccggcg gcaaaacggt cgcgctcaaa
     8341 accgtgggct tgatgatctt gatgacgcaa tgcggcttgc acattccagc cagctccggt
     8401 tcggaagtca gcatcttcga tcaagtatac gccgacatcg gcgacgaaca atcgatcgag
     8461 cagagcttgt ccacattctc atcgcacgtg gtcaacatcg tcggcttcat cgatcgagtg
     8521 actgcaaaat cattagtctt attggacgaa cttggcgcgg gcactgatcc gactgaaggt
     8581 tcggcgctgg cccgatcgat cctcgatcat ttaagcgcca ccgatgcgtt gacgatcgtc
     8641 gcgacgcatt atcccgaatt gaaagtgtgg gcgcattcga cgcaaggcgc catcaatgct
     8701 tcagtggaat tcgatccgga aactttgcgg cctacttaca aactcaccgt cggcctgccc
     8761 ggtcgatcga acgcgtttgc gatcgcgaaa cgcttgggct taaaagatga catcgtctcg
     8821 cacgcgcgat cgatggtggc ggaagacaac ctcaaagccg aagatttgct agctgagatt
     8881 catcagcagc gcgaagcgac cgagcgatcg cgcgcgtccg ccgatcgctt gcgcgacgag
     8941 gcggaaaagt tgacgcggca gttgcgcgat cgattaagtg ccatcgaaca agaacgggct
     9001 tccatcttgg aagcagctca aagcgaatcc caacagctga tcgatacctt caaagatcag
     9061 atcgatcaaa tcaaagcctc gctcttaacg gccgatcaag ccaccattct cacacaattg
     9121 gatcaactcg aagcgcaagc tgaatcgatc gagccgccgg ggccgatcga gaaaccggcg
     9181 atcaacccgc ggcattcgat ccggctgggc gatactgttt atgtgcgcaa gctcaatgcc
     9241 acgggcgatg tcgcttcgat cgatcgagat accgttgaag tcatcgccgg atcgctgcgc
     9301 atgcgcctga agccggacga gatcgaatgg cgatcatcac cgaacacaaa tgttgcggct
     9361 gcgcatgaac gccccgaagc ggtcgaatac gccaacgttc gctcgacgac cgcagcgcac
     9421 atcgagatcg atctgcgcgg cttgcgagcg gatgaagccg tgaccgaagc ggaaaagcag
     9481 atcgatgccg cgtcgctggc cggtctgccg tacgtgcgga tcattcatgg caaaggcacg
     9541 ggcgcgctgc gcaaagcgat ccgtgaggca ttgaaaaata atcctgcgat caaaaacctt
     9601 gagaacggaa aagatgaaga aggcggcgat ggagtgacca tcgcgcattt gaaagtgtga
     9661 gccaagatcg tgtaagcgtc cttcgactga gttgtgaagt cgtggacttc acaactcagt
     9721 cgaaggacgc tcccataatt tatgatggaa caattcctcg caaccgaaac attgatcatt
     9781 ggcttgctgc tgatcgtctc actcgtggcg atggccgtgc ggcgcttgcg tgtgccatat
     9841 accgtagcgc tcgtgatcgt cggcttactg atcacgctac aacaatcgat tcagatcgaa
     9901 ctcacccccg aattaatcct ggccttgttc gtgccgccac tcgtcttcga agccgccttt
     9961 catctgaact tcaaacgcct gcgcaataat ctgatcccga tcctggtgct ggccgtgccc
    10021 ggcgtgctgc ttacaacgat cctcgtcggc ctcatcgttt ccgcgggcgt caatctgcct
    10081 ttatcgactg cgctcgtttt tggcgcgttg atctccgcta ccgatccggt cgccgtcatc
    10141 gctttgttcc gcacactcgg cacgccgcac gacctgggcg tgctggttga aggtgaaagt
    10201 ttattcaacg acggcactgc catcgtcatc ttcaatttga tgattgctgc cgccggtgcg
    10261 gccgtggtga atggcgctgt tcaagcggcc actcatttcg atctcggtcg cgcgatcatc
    10321 gacttcgtga tcatctcgtt aggcggcttg ggcatcggtg tgtttttagg ttggttggtc
    10381 tctcgattga tcgcccggct cgacgactac ttgatcgaaa cgacgctcac taccgtgctg
    10441 gccttcggtg cctatctgat cgccgagcgc ttgcacgtca gcggcgtctt ggcggttgtc
    10501 ggggcgggct tgatcaacgg caacctcgga ccgcgcggca tgtcaccgac cacacgcatt
    10561 gtgttgttca acttctggga atacctcgct tttgtcacga actcgctggt ctttctgttg
    10621 atcggcctgc aagtcaaact aacgcagctc atcgccaacc ttggcccgat cgcgatcgcc
    10681 gtgctggccg tgttgatcag ccgcgcgatt gtggtctacg gcttaacgtg gttgatcaat
    10741 cgcagtaaac taaaagtctc cgctgcttat cagcacgttc aattctgggg cgggctgcgc
    10801 ggcgcgatca gtttagcgtt ggcgttgagt ctgcccgcgg ccttagccga tcgagacttg
    10861 ctgcgcatca tgaccttcgg cgtggtgtta ttcacgctcg tgggtcaagg cacgacaatg
    10921 cagttcctga ttaaaaaatt gaaactgacc gaacgcatcg aaggcaaact cgaatttgag
    10981 cggcggcatg gccgcttgtt agccgcccgc gccgaacgcg atcgcttgga gcgtctgcat
    11041 cgcgagggca cactgtccga atccacctgg gaacaactgg cgcctgatct cgatcaacac
    11101 atccgatcgc agcttgccgc tcagcgcgaa ttgctggcac agcatccggt gctgcaaacc
    11161 gaagagctgg aagatgcaaa acgtgaaggc ctgcgcgcac ggcgagcgac cttgtccacc
    11221 cttctgcgcg atggtgtgat ctccgagcgt gcttacgaag aactcgtcgc cgagatcgat
    11281 gcaaagatcg aaaatcaaac cgaaccagat tcagacgagg cgcagcctgc agattgacag
    11341 gatgtttgga ttgaggccat cttcgctgga cgctatctcc gatcatttga tcttcctgta
    11401 cggtctgcgc gcgggccgtg tcgcagccga tcgaattatt cgccgcatcg atcagtatcg
    11461 agcgatcatc actccacgcc caagcagaaa tactttctct cagcatgatg cgatgttgat
    11521 cacctacggc gatcaactgc gcgcctcaaa cgaatcgccg ctccacacat tgaccgattt
    11581 ctgtcagaag catctctcag gattgctcaa tggcattcac ctactgccat ttttcccgta
    11641 ctcctccgac gacggttttt ccgttatcga ttaccgacaa gtcgatccac gacttggcgc
    11701 gtgggacgac atcgatcgat tgagtcaaca ttttcggctg atgttcgacg cggtgatcaa
    11761 tcacatctca gcggaaagtg aatggttcaa aaagttcctt caagatgatc cgaagtatca
    11821 agattacttc attgtcgtcg atggcaatcc tgatctc
//
LOCUS       DASRTJ010000354         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_96692,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000354 DASRTJ010000000
VERSION     DASRTJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96692"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1617)
                     /locus_tag="VFF70_07230"
     CDS             complement(<1..>1617)
                     /locus_tag="VFF70_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZY44525.1"
                     /translation="CGQSPLTGIWNEANLGGHWGAELRFAGFDGIVISGKAANPSYLY
                     INGETNTIELRDGAHLWGKDTFEVFDQLIVETDEKARAAIIGVAGEKLVKIAGIASGG
                     HSHSRLAARGGMGALMGSKNLKAIVMRGKEKPQYADTKGFHSTVKESNKFIMDHGPAE
                     SLHLVGTAGGHPTTDKFGDNAIMNWRGGNWTEGTIKTSGKTMAETILTKHTFCYACPI
                     GCGKAIEVKDGPYKGTWGEGPEYETLCGFGSNLQCDDLSAIAAMNDWCNRYAIDVIST
                     SSVIAWAFEAFEKGAITTKDTGGIELKWGDAEAIIAMIHKIGKREDIGDVLAEGVRYA
                     SRKIGHGTDKYAMHVKGLEMPYHDPRGFVSMGANYATASRGACHLEAISYWRGVGIEW
                     QGWQDGDRQEWIDSKRFDSSLGAQQAIDFQNYMTTYNPLGLCKFITKGSFTPDQTAEL
                     VNKALGWNWTGQDVIDTGARLFNFKRLINNRLGISAKDDVLPSRLTTEPRPTGTAAGV
                     LPDMKLMMKDYYRIRKWDANGVPTKEAMLPV"
BASE COUNT          300 a          514 c          401 g          402 t
ORIGIN      
        1 actggtagca tcgcctcttt cgtcggcaca ccattcgcgt cccacttgcg gatgcgatag
       61 taatccttca tcatcaactt catatcgggc agcacgccag ccgctgtgcc ggtcggtcga
      121 ggttcagtgg tcaatcggct cggcagcaca tcatccttcg cgctgatgcc caatcgattg
      181 ttgatcagcc gcttgaagtt gaacaacctc gcgcccgtat cgattacatc ttgccccgtc
      241 caattccagc cgagcgcttt gttcactaac tctgccgttt gatcgggggt gaacgatcct
      301 ttggtgatga acttgcacaa accaagtgga ttgtacgtcg tcatgtaatt ctgaaaatcg
      361 atcgcctgct gcgcgccgag cgatgaatcg aagcgcttgc tgtcgatcca ctcttgtcga
      421 tcgccatcct gccagccctg ccattcgatg ccgacgccgc gccaatatga aatcgcttcg
      481 aggtggcatg caccgcgcga ggcggtcgca taattcgcgc ccatgctgac gaagccgcgc
      541 ggatcgtggt acggcatctc caaacctttg acgtgcatcg cgtacttgtc tgtgccatga
      601 ccaattttgc gcgaagcgta gcgtacgcct tcggccaaca catcgccgat gtcttcacgc
      661 ttaccgatct tgtgaatcat cgcgatgatc gcttcggcgt cgccccattt caattcgatg
      721 ccgcccgtgt ccttggttgt gatcgcgcct ttctcgaacg cttcgaacgc ccacgcgatc
      781 acactcgagg ttgagatcac gtcgatcgcg taacggttgc accaatcgtt catcgccgcg
      841 atcgcactga gatcatcgca ttgcagattc gatccgaagc cgcacagcgt ttcgtattca
      901 ggcccttcgc cccacgtccc tttatacggt ccgtctttaa cctcgatcgc tttcccgcac
      961 ccgatcggac aggcgtaaca aaatgtgtgc ttcgtcaaga tcgtctcggc cattgtcttg
     1021 cccgatgttt taatcgtgcc ttcagtccaa ttgccgccgc gccaattcat gatcgcgttg
     1081 tcgccaaact tatcggtcgt gggatggccg cccgctgtgc caacaagatg cagcgattca
     1141 gccggaccgt gatccatgat gaacttgttg ctctctttga cggtcgagtg aaaacctttc
     1201 gtgtcggcgt attgcggctt ctctttgccg cgcatgacga tcgctttgag attcttcgag
     1261 cccatcaacg cgcccatgcc gccgcgagct gccaatcgac tgtgcgaatg tccgcccgac
     1321 gcgatgcccg cgatcttcac caacttctcg cccgccacgc cgatgatcgc cgcgcgcgct
     1381 ttttcgtccg tctcgacgat cagttggtcg aacacttcga aagtatcctt accccacaga
     1441 tgcgctccgt cccgcagttc gatcgtgttt gtttcgccgt tgatatacaa gtaactgggg
     1501 ttcgctgcct tgccgctgat cacaattcca tcgaatccgg cgaatcgcag ttccgcgccc
     1561 caatgtccgc cgagattggc ttcattccaa atgccggtga gcggcgattg gccgcac
//
LOCUS       DASRTJ010000355         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_324309,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000355 DASRTJ010000000
VERSION     DASRTJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324309"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..891
                     /locus_tag="VFF70_07235"
     CDS             172..891
                     /locus_tag="VFF70_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016815780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HZY44526.1"
                     /translation="MNSFVVVPTYNEADNIASFVAEIFKLNLPELNVLIVDDESPDGT
                     GQIADRLAEQYRDRLFVLHRSGLRGLGRAYVDGFHWAIEHGAERIIQMDADFSHSPSY
                     IPQFLEKSNAYDVIIGSRYVPGGSLDKEWEQGRVFLSAWANFYARNILRLKVHDATAG
                     FKCWTRSALEKIQLDRVRSNGYVFQVEMAYLSQKLKLRVLEIPIHFEDRRIGHSKMSI
                     PVKLEAAWRVWQVWWRHKNVK"
     gene            1027..>4773
                     /locus_tag="VFF70_07240"
     CDS             1027..>4773
                     /locus_tag="VFF70_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZY44527.1"
                     /translation="MLQRRKSDLLILLGLFLLPLIPFWSVTIGGQTLLPVDNLYQFAP
                     WKSAAEQFNAQVPQNQLLSDLILENYAWKHYIVQSIQQRELPLWNPNLFAGAPFLANG
                     QHSAYYPFSILFYLLPLTAAYGWFTVTQLFLAGAFMYLLCRVLGIGKLGSLFAAITYQ
                     LSGFYIVSVVFTMIIAAAAWLPLLLVMIELIIKQHPTFGRPSTWPWVLIGSIGLACQI
                     MAGHIEITYYTLLIMALFAAWRLLMRPQTANSRPQYVIAVHRPRTDHQQVNISGLWSS
                     VSGLITRSRRPLIALLVLVILGVGLAAIQLLPYIEILPQNFRVGAATFDQVRSYAYPP
                     RHILEMLMPNFFGSPAQHSIFDIFTFQNISLFHDFSAHNPQGIYSTMWGIKNYVEGGA
                     YLGLLPLLLALFAFARRKTFSSTVHIWFFALLTLVSLAFMFGTPLYAILYYGLPGIDQ
                     LHSPFRWVFPFTISIAVLAAYGIDALMRDRERDRYSWRSRGPIGWLALKAPISFRSIM
                     AGVAFWIGSILFAILLLSRLIFPAQSIDLADKLLHSLVLADTAFPNAQLFYSFEWRNI
                     LLFALFLMSSGIVLRVSLCDIYIRNFLKFLKPTNRIGHVAVWKPLLLFVLVLDLFAGA
                     IGFNPSVDPKLLDYTPPVVKFLQQDTSLWRFTTFDPHGDKPFNANAAWSYNFQDVRGY
                     DSIILKQYANYMSLIDTQAELQFNRIAPLTTYGGLDSPMLDLLNVKYVITDPATPIES
                     PKYKLVYDAEVKVYENLGVMPRAFTMPSDCAIGVDDQLEGLKQHDPRTGVIIGNADFS
                     RQPTEVGAPCSLQSATITDYRANDVTLRQVNVTQPSFLVLADTYFTGWQAFEVKPDQS
                     ETEIQIVRADGNFRAVSIDAGDHTIHFKYSPWSFKLGLFISFMGWMLVVFTLGIWVWR
                     LAYREEEHAGTTVRRVAKNSIAPMLLNLFNRLIDFAFAALMLRILQPENAGNYYFAVV
                     IVGWFEILMNFGLNTLLTRAVARARSDANKYFSNTSILRLILAGVSIPLVLIVIVVLH
                     SAFTLTTETVIAIILLTLAQIPSSLSTGITSMFYAVEKAEYPAVIGVVTVLLKVLFGV
                     PVLILGGGIIGLAAVSLLVNLITFVVLSRMLIRNVVRPHLESDPALRRGMLRESFPLM
                     LNHLLATLFFKIDVPLLQAQQGSTTVGWYSTAYKWIDALNIIPAYSTLALFPVMSRQA
                     LEDKDALMRSTRLGIKLLVMIALPLAVVTTFIAPLLVLILGGPSY"
BASE COUNT          993 a         1361 c         1190 g         1229 t
ORIGIN      
        1 caacaggcac aagcgcttct ggcaaagttg aagaagtagc acatatcaac aagcctctga
       61 gaattcagag gctttttgtt tcctttgcac attgcacaca cttgcatcgc actgcgtttg
      121 ttgcagtgca ggtgttgttc attgttaatt gctcatgtat aatcccccgc catgaactca
      181 tttgtcgtgg ttcccaccta taacgaagcc gataacattg ctagcttcgt tgcagaaata
      241 ttcaagttaa acttacccga actcaatgtg ctgatcgtcg atgatgaatc accggacggg
      301 acgggccaga tcgccgatcg gttggcggag caataccgcg atcgattgtt cgtgctgcat
      361 cgatccggcc tacgcggact cggtcgcgcg tacgtcgatg gcttccactg ggcgatcgag
      421 catggcgccg agcgcatcat ccaaatggat gcggacttct cgcattcacc gagttacatt
      481 ccgcagtttt tggagaagtc caacgcatac gatgtgatca tcggatcgag gtacgtgccc
      541 ggcggctcgc tcgataaaga gtgggaacaa ggccgcgtgt tcttatccgc gtgggcgaac
      601 ttttacgcgc gcaatatttt gcgcttgaaa gtccacgatg cgacggcagg ctttaagtgc
      661 tggactcgat cagcgctgga aaaaattcaa ctcgatcgag ttcgatcgaa cggttatgtg
      721 ttccaagtcg agatggccta cttgtcgcag aaattgaaac tgcgtgtttt agaaataccg
      781 atccactttg aagatcgacg catcggccat tccaaaatgt cgatcccggt gaaactcgaa
      841 gccgcgtggc gcgtgtggca agtgtggtgg cggcataaga atgtcaaatg aaaagtgagt
      901 aaatgacaaa tgcataatgc gtgacaacgt gataacgtga tgcgtgattc actgtttact
      961 tttcactcat cacttgcagc ttgcaacctg caccttgcaa cttctaactt ccaacatcca
     1021 acttccatgc ttcaacgtcg caaatccgat ctcctcatcc tcctcggcct cttcctcctg
     1081 ccgctcattc ccttttggtc ggtgacgatc ggcggacaga cattattgcc ggtcgataat
     1141 ctatatcaat tcgcgccgtg gaaatcagcc gccgagcaat tcaacgcgca agtgccacag
     1201 aatcaactcc tcagcgatct gattctcgag aactacgcgt ggaaacatta catcgttcaa
     1261 tcgatccagc agcgcgaact gccgctgtgg aatccgaatc tctttgcggg cgcgccattc
     1321 ttggcgaatg gacaacattc ggcatattac ccgtttagca ttctctttta tctgctgccg
     1381 ctcaccgcgg cttacggctg gttcaccgtc acgcagttat tcctcgcggg cgcgttcatg
     1441 tatctcttgt gccgcgtgtt gggcatcggc aaattgggca gcctcttcgc cgcgatcacg
     1501 tatcaactca gcggcttcta catcgtcagc gtcgtcttca cgatgatcat cgccgccgcc
     1561 gcctggctgc ccttgttgct ggtgatgatc gaattgatca tcaagcagca tccaacattt
     1621 ggtcggcctt cgacgtggcc gtgggtcttg atcggatcga tcggcctcgc ctgtcaaatc
     1681 atggccggtc acatcgagat cacgtattac acattattga tcatggcgct gttcgcggcg
     1741 tggcgattgc tgatgcgacc gcagacggcg aacagccgac cgcagtatgt catagccgta
     1801 catcgtccaa gaactgatca tcaacaagta aacatcagcg gtctttggtc ttcggtctcc
     1861 ggtctcatca ctcgaagccg tcgtccgctc atcgccctgc tcgtcttggt catcctcggc
     1921 gtcggcctcg ccgcgattca actgctgccg tacatcgaaa tcctgccgca gaactttcgc
     1981 gtcggcgccg ccacgttcga tcaagtccgc agctacgcat atccgccgcg ccacattctc
     2041 gaaatgctga tgcccaactt cttcggcagc ccggcgcagc attccatctt cgacattttt
     2101 acctttcaaa acatttcgct cttccacgat ttcagcgcgc acaatccgca gggcatttac
     2161 agcaccatgt ggggcatcaa gaactacgtc gaaggcggcg catatctcgg cctgcttcca
     2221 ttgttattag cgctctttgc gttcgctcgc cgtaaaacat tttcatcaac cgtacacatc
     2281 tggttcttcg cccttctcac actcgtatca ctcgccttca tgttcggcac gccgctgtac
     2341 gcgattttgt attacggcct gcccggcatc gatcaactgc attcgccgtt ccgctgggta
     2401 tttcctttca cgatttcgat cgcagtgctg gccgcgtatg gtatcgatgc gttgatgcgg
     2461 gatcgtgagc gcgatcgata ttcatggcga tcgcgtggcc cgatcggttg gcttgcgctt
     2521 aaagcgccga tctcctttcg atcgatcatg gctggcgttg cattctggat cggatcgatc
     2581 ctatttgcga tcttgctgct ctctcgattg atcttcccgg cccaatcgat cgatctcgcc
     2641 gacaaactgc tgcattcatt agtcttagcg gacacggcct ttcctaacgc gcaacttttc
     2701 tattcattcg agtggcgcaa cattttattg ttcgctcttt tccttatgtc gagcggcatc
     2761 gtactgcgcg tttctttatg cgacatctac atccgcaatt tcttgaagtt cctcaagccg
     2821 acaaaccgaa tcggccacgt cgccgtttgg aaaccgctgc tgctcttcgt cctcgtgctc
     2881 gatctgttcg cgggcgcgat cggcttcaat ccgtccgtcg atccgaaact ccttgactac
     2941 acgccgcccg tcgtgaaatt cttacagcaa gacacctcat tgtggcgctt caccacattc
     3001 gatccgcatg gcgacaaacc attcaacgct aacgccgctt ggtcttacaa ctttcaggat
     3061 gttcgcggtt acgattcgat catcttgaag cagtatgcca actacatgag tctgatcgat
     3121 acacaagccg agttgcaatt caaccgcatc gcaccgctca ccacctacgg cggcctcgat
     3181 tcgcccatgc tcgatctgct caacgtgaag tacgtcatca ccgatcccgc cacgccgatc
     3241 gaaagcccga agtacaaact cgtctacgac gccgaagtga aagtgtacga aaatctcggc
     3301 gtgatgccgc gcgcgttcac gatgcccagc gattgcgcaa tcggagttga tgatcaactt
     3361 gaaggcttga agcagcatga tccgcgcaca ggagtcatca tcgggaacgc cgactttagt
     3421 cggcaaccga ctgaagtcgg tgctccgtgt tcgttacaat ctgccaccat cactgactat
     3481 cgagcgaacg atgtcaccct gcggcaagtc aatgtgactc aacccagttt cctcgtgctc
     3541 gccgatacct atttcaccgg ctggcaagcc tttgaagtga agcctgatca atcggaaacg
     3601 gaaatccaga tcgtgagagc ggacggaaat ttccgcgcgg tatcgatcga tgcgggcgat
     3661 cacacgatcc atttcaaata cagtccgtgg tcgttcaagt taggcttgtt catttcgttc
     3721 atgggctgga tgttggtcgt gttcacgctg ggcatttggg tgtggcgctt ggcctatcgc
     3781 gaagaagaac acgcgggcac gaccgtcagg cgcgtagcaa aaaattcgat cgcgccaatg
     3841 ttgttgaact tgttcaatcg cctgatcgat ttcgctttcg cggcgttgat gctgcgcatc
     3901 ttgcagccgg agaatgcggg caactattat ttcgccgtcg tgatcgtcgg ctggttcgaa
     3961 atcttgatga acttcggatt gaacacgctg ctcacccgcg ccgttgcccg cgcgcgatca
     4021 gatgccaata aatatttctc caacacatcg atcctgcgtt tgatactggc aggtgtgtcg
     4081 atcccgttgg tgttgatcgt catcgtcgtc ttgcacagcg cgttcacgtt gaccaccgaa
     4141 accgtcatcg cgatcatcct gctcacgctg gcgcagattc ccagcagcct ctcgaccggc
     4201 atcacgtcga tgttttacgc ggtcgagaaa gccgaatatc cggcggtcat cggcgtcgtc
     4261 acggtcttat tgaaagtgtt gttcggtgtg ccggtgttga tcctgggcgg cggcatcatc
     4321 ggcttggcag ccgtgagttt gttagtcaac ctgatcacgt ttgtcgtgct tagccgcatg
     4381 ctgattcgca atgtcgtgcg tccacatctt gagagcgatc cggctttgcg gcgcggcatg
     4441 ctccgtgaat cgttcccgtt gatgctgaat catttgctgg caacattgtt cttcaagatc
     4501 gatgtgccgc tattgcaagc gcagcaaggc tcgacgaccg tcggctggta cagcaccgcc
     4561 tacaaatgga tcgatgcgct caacatcatt ccggcttatt cgacgctagc gttgtttccg
     4621 gttatgtcgc ggcaagctct cgaagacaaa gacgcgctga tgcgatcgac gcgattgggc
     4681 ataaagttgc tggtgatgat tgccttgcca ctcgctgtgg tgacaacatt tatcgcgccg
     4741 ttgctcgtgt tgatccttgg tgggccgtca tac
//
LOCUS       DASRTJ010000356         1969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_349762,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000356 DASRTJ010000000
VERSION     DASRTJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349762"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFF70_07245"
     CDS             complement(<1..285)
                     /locus_tag="VFF70_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44528.1"
                     /translation="MKCTADIDGATFYSQGCKLLGGFYRAAGDSPRPTVVLCHGLPGV
                     EKNLDIAYGLRDAGWNCLYFHYRGCWGSEGTYSLNGLLDDAHAVTDWLVQQ"
     gene            complement(282..1007)
                     /locus_tag="VFF70_07250"
     CDS             complement(282..1007)
                     /locus_tag="VFF70_07250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44529.1"
                     /translation="MSQSNITKHADYGIDAPGVVRGLLISGVATISLGVLLATSSAFL
                     LICGLLAGAAGLISASIMLWSSKVGKFRMRDRLINSLEWRGDEKVLDVGCGHGLLLIA
                     AAKQLKTGKAIGVDIWSQVDQGSNRPEATIENAHIEGVTDRVEVRDGDARHLPFEDNS
                     FDVIVTSLVIHNIRDRFGRDQAIHEIARVLKPGGRIAIHDMAHLRDYAQVLRNNGLAD
                     TAFSERIFLFNVFTHILTANKPI"
     gene            complement(1094..1894)
                     /locus_tag="VFF70_07255"
     CDS             complement(1094..1894)
                     /locus_tag="VFF70_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44530.1"
                     /translation="MTNNAQDVWANADTYEHYVGRWSRLAAKQFVKWLNLPSNGSWLD
                     VGCGTGALSQTILNVASPQKVTGIDRSDGYVEFARRQITDSRAAFEVGDAQDLKQFED
                     KSFDAAVSSLVLNFVPQPDRMVAEMKRAVKTNGVVALYVWDYAGKMQLLRHFWNAAAA
                     LDPAAIELDEGRRFPICNPNRLTDLFRSAGLQNIETRPIDVDTHFKDFDDYWTPFLGG
                     QGPAPGYCISLSEDRRVSLREKIRSSLPFALDGSIPLVARVWAVCGYC"
BASE COUNT          472 a          545 c          491 g          461 t
ORIGIN      
        1 ttgttgaacc agccaatcgg tcacggcatg cgcatcatct aacaagccat ttaacgaata
       61 agttccttct gatccccaac agcctcgata atgaaagtac aggcagttcc agcctgcgtc
      121 tctcagccca tacgcaatat cgagattctt ttcaacgccg ggcaagccgt ggcaaagtac
      181 aactgtcggt cgaggtgaat ctccggccgc tcgataaaat ccgccaagca atttgcaacc
      241 ctgtgagtag aaagtggcgc cgtcgatgtc ggctgtacat ttcatattgg cttattcgcc
      301 gtcagaatat gtgtgaacac attaaagaga aagattcttt cggaaaatgc tgtgtcagct
      361 aagccgttgt tacgcaacac ttgcgcgtaa tcacggagat gtgccatatc gtgaatggcg
      421 atgcgaccgc cgggtttcag gacgcgggcg atctcatgta ttgcttgatc gcgtccaaat
      481 cgatcgcgta tgttgtgaat gacgaggctg gtcacgatca cgtcgaacga attgtcttca
      541 aatggcaagt gccgtgcatc gccgtctctc acttcaactc gatcggtcac gccttcgatg
      601 tgcgcattct cgatcgtcgc ttccggtcga ttgctgcctt gatcgacttg cgaccaaatg
      661 tcgacaccaa ttgctttgcc ggtcttgagt tgtttcgcag cggcaatcaa caatagacca
      721 tgcccgcagc cgacatccaa aactttttca tcgccgcgcc actcgagcga gttaattaat
      781 cgatcgcgca tgcggaactt gccaactttg ctgctccata acattatcga tgcagagatc
      841 aaaccagcgg ctcccgcgag gagtccacaa atgagcagaa aagcagatga cgttgccaga
      901 agaacgccga gactgatcgt tgccacaccg ctgatgagta atccgcgcac aacgcccggt
      961 gcatcgattc catagtccgc gtgtttggtg atgtttgact ggctcaatat acgctccgcg
     1021 aatgtgctga ttatagcatg cggacgttcc atcggaacgt ccgcttaagt caatcacagg
     1081 ggaaaagtac gattcaacaa tagccgcaca cagcccacac gcgcgcgacc aatgggatcg
     1141 agccatcgag cgcgaacggc aaacttgatc ggattttctc gcgcaacgac acgcggcgat
     1201 cctcgctcaa cgatatacag tagcctggcg caggcccttg tccgccgagg aacggtgtcc
     1261 aataatcatc gaagtctttg aagtgagtgt caacatcgat tgggcgcgtt tcaatattct
     1321 gcaggccagc cgatcgaaac agatcggtta atcgattcgg attgcagatc ggaaagcggc
     1381 ggccttcatc tagctcgatc gccgccggat cgagcgcagc tgcggcgttc cagaaatgtc
     1441 gcaataactg catcttgccc gcgtaatccc agacgtagag cgcgaccacg ccgtttgttt
     1501 tcacagcgcg tttcatttca gccaccatac gatcaggttg tggcacaaag ttcaagacga
     1561 ggctggacac tgccgcatcg aaagatttat cttcgaattg ttttaggtct tgcgcgtcac
     1621 caacctcgaa cgccgcgcgt gagtctgtga tctgccttcg ggcaaattca acatagccat
     1681 cggatcgatc gatccccgtg actttttgag gcgaggcaac atttaagatc gtctggctca
     1741 acgcaccggt gccgcagcct acatcgagcc atgatccatt tgaaggaaga ttcagccatt
     1801 tgacgaactg cttggcggct agccggctcc agcgaccgac gtagtgctca tacgtgtcgg
     1861 cattcgccca gacatcttga gcgttattgg tcatgatttc tcctccccgg gatcgtcatg
     1921 acgatagaga cgtttcgccg aaacgtctct acgatagatc cccttgtcc
//
LOCUS       DASRTJ010000357         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_248695,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000357 DASRTJ010000000
VERSION     DASRTJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248695"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFF70_07260"
     CDS             <1..499
                     /locus_tag="VFF70_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44531.1"
                     /translation="SQHRRYSARGLRVWAWSALLFLSAATYILLLSEQSTRMLAQLPP
                     SFRDGAEIAALDWLNQQATYDDVVLSFYSTGNFLPTRVAALVFLGHGPETAFSDDKRQ
                     LVTQFYNPATSEAWRRSFLNAWPITYVFNGPLEKQLGAIDLSGLANLTLVYDHAAYQI
                     YRVSR"
     gene            496..2790
                     /locus_tag="VFF70_07265"
     CDS             496..2790
                     /locus_tag="VFF70_07265"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZY44532.1"
                     /translation="MRSILRLYLDRYWPILLLIALPIVLLAPIVLGRVLYWGVPLLQF
                     YPWQHAAIDAYQHGQLPLWNSLVGSGAPLAANLQTGAFYPLNILYLILPTEYAMGYTT
                     ILHVVLAGLFMYAFIRSLKLDRFSSVVAAIAFQLCGFLIARLSFFSITATLPWLAAWL
                     WRTERLVASGQRLAVRNALLLALAIGLGILAGHAQTAVYGLIFVTLYFIWRMITLSPR
                     HLVTLSACLFIIAVTLGLGLAAIQLLPSLELARESQRAVGLDFTKVMTHSYWPPRLLT
                     MLSPDFFGNPAQNNFWGYDNYWENAAFIGVIPLLLALWVIKLHVASFKLHRLKASNPT
                     SNLQSPISNLQSPTSYFILSFVVSLILAFGWFTIIYPFLYNTVPGFKLFQGPDRWLSI
                     TSIALCVLAGFGAQRLIDYGFPRRAALRLILLGLALSIAGVGSLFIFRDKFATFGPSI
                     LRSGILLIVSGFLFKLAIKNQMSKILLIAVISLDLIVAHISLNPTIDPLLYRAANPVA
                     DAIKAAGISGRIFYFDQDEAATKFGVYLAHGNPPRFDGFGSSDFNYWLSEREALLPNV
                     GMIDGIASANNFDSLIIGRYQTLLDQINQLPLDQALSELARMNVAYIISPRDLNLPIV
                     YRATNVMIYRNEKVMPRAWLAPIDSDLNQVTAILSGSSIDSLTDSSNAVTIRAASPVE
                     AWLILSDTYYPGWQATLDGQVTDIQLANQSFRAVKFPAGAHQVEFRYEPNSVTIGATI
                     TLISVVVLVAGLAVTLRRRADEAH"
     gene            2777..3871
                     /locus_tag="VFF70_07270"
     CDS             2777..3871
                     /locus_tag="VFF70_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44533.1"
                     /translation="MKRIRPWHIVALIGLIYVAITFARNNFDPLRFALIGSQYDPGIP
                     NGTPGYDGQFAYQIARDPINGWTKIDVSAYRYQRIVYPIAARVLALGNVDLIPWTLIA
                     INIAALIAGTWFTEMILEHYQVSRWYALVYGLNAGTLMSVRLDLTEPLAYALAQLGVL
                     LLLQDRSRWAALAFAFAVLTKETTLLFVAGVAVTYLIDRRGRKLIELITISISPFAIW
                     QIVLLNRFGQLGIGSGGALSTPFEIIPLRGLWSIASIDVRVFLLMTIIMLPMAVLPMA
                     FALRSVWRDARTKKFDLAAALLLLNAFIILFTPQSTFREPLAMARFAVGLIATTLTYA
                     AARKSKRGLNYALLWMFTLGFALNEAQLPN"
     gene            3885..4622
                     /locus_tag="VFF70_07275"
     CDS             3885..4622
                     /locus_tag="VFF70_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HZY44534.1"
                     /translation="MRELKTVVIIPTYNEKENIEILLADLLALPVNLSVIVVDDGSPD
                     GTGQLADQQAAKTDRVQVIHRASKLGLGTAYIAGFKRALSNGFDRIMTMDADFSHHPK
                     YIPAMIELSHECDMVIGSRYVKGGGTLNCPWKRRMLSQGANAFTKFMLSQRARDCTAG
                     FRCYRREVLESIDLDSIFSNGYSFLIEMLYYVQRRGWQIGEVPIVFEDRQRGKSKISQ
                     NEVSRALQTVLRLEMRRMRGMFQREAR"
     gene            4622..>4962
                     /locus_tag="VFF70_07280"
     CDS             4622..>4962
                     /locus_tag="VFF70_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44535.1"
                     /translation="MEYVRCNLCGQDKTIPRFPSTVKPDTVPQNVEAFRCTSPGYGRH
                     FAIVSCQNCGLVYTNPRLDGKQIIDSYVAVEDPLYLEERDGRVLTFERHLKPLEKLHA
                     PPGKLLDVGAYT"
BASE COUNT          980 a         1264 c         1349 g         1369 t
ORIGIN      
        1 gtcccaacat cgacgctatt cggcgcgcgg cctgcgggtt tgggcctggt cagctttgct
       61 gtttctttcg gcggctactt acattctctt gttgagtgaa caatcaacac gtatgctggc
      121 acagctgccg ccgagttttc gcgatggcgc tgagatcgcg gcgctcgatt ggttgaatca
      181 acaagccacc tatgatgatg tcgtgttgtc tttctatagc acaggtaact ttttaccgac
      241 gcgcgtggcg gcacttgtct ttttgggtca tggcccggag actgcgttta gtgatgataa
      301 gcgccaactc gtcactcagt tttacaatcc ggccacatca gaagcttggc gtcgatcgtt
      361 tttgaatgct tggccgatca cttacgtgtt taatggtccg ctcgaaaaac aattgggcgc
      421 gatcgatcta tcgggcttgg ctaatctaac gttggtgtat gatcacgctg cttatcaaat
      481 ctatcgagtg agccgatgag atcgatccta cgtctatatc tagatcgata ttggccgatc
      541 cttcttttga tcgcgctccc gatcgtgctg ctggccccga tcgttttggg gcgagtcttg
      601 tattggggcg tgccgctgct gcaattttat ccgtggcagc acgcggcgat agatgcgtat
      661 caacacggac aattgccact atggaattcg ttggtcggca gcggcgcgcc gttagctgcc
      721 aaccttcaga ccggtgcatt ttatccgctc aacatcttgt atttgatttt gccgactgaa
      781 tatgcgatgg ggtacacgac gatcttgcat gtggtactcg cggggttgtt catgtatgcg
      841 ttcattcgat cgctgaaact cgatcgattt tcttccgttg tggcggcgat cgcttttcag
      901 ttgtgcggtt tcttgatcgc gcggctgagt ttcttcagca tcactgcgac cttgccgtgg
      961 ctcgcggcgt ggctctggcg gacggagcgg ctagtggcta gcggccagcg gctagcagtt
     1021 cgaaacgcgt tgctcttggc gctcgcaatt ggtttgggca ttttagcagg ccatgcgcag
     1081 acagctgtgt atggcttaat ctttgtcact ctgtatttta tctggcgaat gatcaccttg
     1141 tcgccccgcc acctcgtcac cttgtcagct tgcctgttca tcattgcggt aacactcggc
     1201 ctcggtctcg ctgcgataca attgctgcct tcattagaac tggctcgcga atcgcagcgc
     1261 gcagtcggac tcgacttcac caaagtgatg acacattcct actggccgcc gcgtttgctg
     1321 acgatgcttt cgccagattt ctttggcaat cccgcgcaaa acaatttttg gggctacgat
     1381 aattattggg agaacgccgc attcatcggc gtgattccgt tgttattggc attgtgggtt
     1441 atcaagttgc acgtcgcaag tttcaagttg caccgattga aagcgagcaa tccaacttcc
     1501 aacctccaat ctccaatctc caatctccaa tctccaactt cgtacttcat tctttctttt
     1561 gtcgtctcgc tcatcttggc tttcggttgg ttcacgatca tttatccgtt cttgtacaac
     1621 actgtgcctg gcttcaaatt attccaaggg ccggatcgat ggttgtcgat cacgtcgatc
     1681 gcgttgtgcg tcttggcagg ctttggtgcg cagcggttga tcgattatgg ttttcctcgc
     1741 cgcgctgctc tgcgattaat cttgttgggc ttagcgttgt cgatcgcggg cgttggatca
     1801 ctcttcattt ttcgtgataa gttcgctaca tttggtccta gcattttgcg ctctggcatc
     1861 ttattgattg tcagcggctt cttgtttaaa ttagccatca aaaatcagat gtcaaagatt
     1921 cttttgatcg ctgttatctc actcgatctc atcgtggcgc atatctcact caatccgacg
     1981 atcgatccgc tgctttatcg cgcggccaat cctgtcgcgg acgcgatcaa agccgctggc
     2041 atctcgggcc gcatcttcta tttcgatcaa gatgaagcag cgaccaagtt cggcgtttat
     2101 ttagcacacg gcaacccgcc ccgcttcgat ggttttggat cgagtgattt caattattgg
     2161 cttagcgagc gcgaagcatt gctgcccaac gtcggcatga tcgatggcat tgcatcagcc
     2221 aacaactttg actcgcttat tatcggccgt tatcaaacct tattagatca aatcaatcaa
     2281 ctgccgctcg atcaagcgct atccgaattg gcgcgtatga acgtggccta tattatcagc
     2341 ccgcgcgatc tgaatttgcc gattgtctat cgcgccacaa atgtaatgat ctatcggaat
     2401 gagaaggtta tgccgcgagc ctggctggcg ccgatcgatt ccgatctcaa tcaggtcacc
     2461 gcaatattga gtggatcgtc gatcgattct ttgaccgatt ctagcaatgc cgttacaata
     2521 cgcgccgcgt cgcccgtgga ggcctggctg attttatcgg acacatatta cccgggttgg
     2581 caggccaccc tcgacggaca ggtgacggat attcaactcg ccaatcaatc ttttcgtgcc
     2641 gtaaaattcc cggcgggcgc tcatcaagtt gagtttcgat acgaaccgaa ttcagtgacg
     2701 attggtgcga cgattacctt aatctctgtg gtggtgctcg ttgcaggact agcagttacg
     2761 ctccgacggc gggccgatga agcgcattag accgtggcac attgtcgcgc tgatcggctt
     2821 gatctatgtc gcgatcacgt ttgcgcgcaa caatttcgat ccgttgcgct ttgcgttgat
     2881 cggctcgcaa tatgatccgg gcattccaaa tgggacaccg ggctacgacg gccaatttgc
     2941 ttatcagatt gcacgtgatc cgattaatgg ctggacgaag atcgatgttt cagcttatcg
     3001 ttatcagcgg attgtttatc cgatcgctgc gcgcgtcctg gcgttgggca acgtcgatct
     3061 gattccgtgg acattaatcg ctatcaatat tgcggcgctg attgccggaa cgtggttcac
     3121 ggaaatgatt ttggaacatt accaggtcag tcgctggtac gcgttggtct acgggctaaa
     3181 cgccgggacg ttgatgtcgg tgcgtttgga tttgaccgaa ccgctggcct acgcgctggc
     3241 gcagctgggc gtgctgttgc ttcttcaaga tcgatcgcgt tgggcggcgt tggcctttgc
     3301 gttcgcggtg ttgactaaag agacgacgct gttgtttgtt gccggagtag cggtgactta
     3361 tctgatcgac cgtcgagggc gtaagctaat cgagttgatt acgatctcga tctcgccttt
     3421 cgcgatatgg cagatcgtct tgctgaatag atttgggcag ttggggattg gatcgggcgg
     3481 ggcgctgtcg acaccgttcg agattatccc gctgcgcggc ttgtggtcga tcgcgtcgat
     3541 cgatgtgcgg gtctttctgc tgatgactat catcatgttg ccaatggcgg tgctgccaat
     3601 ggcgttcgcc ctccgatcgg tgtggcgcga cgcgcggaca aaaaaattcg atctagccgc
     3661 tgcgctgttg ttgctgaatg ccttcattat tttgttcacg ccgcaatcga cgttccgcga
     3721 gccgttggca atggcgcgct ttgcagttgg attgattgcc actacactga cctatgcggc
     3781 ggcgcgcaaa tcaaagcgcg gcttgaatta tgccttgttg tggatgttca cattaggttt
     3841 tgccttgaat gaagcgcagc tgccgaactg atgagtgata cgtgatgcgt gaacttaaaa
     3901 ccgttgtgat cattccaact tataacgaaa aagaaaatat cgaaatcctg ctggcggatt
     3961 tgttggcttt gcccgtcaac ttaagcgtga tcgtcgtcga tgacggatcg cccgacggca
     4021 ccggacagct ggccgatcaa caggcggcca agaccgatcg ggtgcaggtg atccatcgag
     4081 cgagcaagct aggcttgggc accgcttaca tcgccggctt taagcgcgca ttgtcgaacg
     4141 gctttgatcg catcatgacg atggacgccg atttctcgca tcatcccaaa tatattccgg
     4201 cgatgatcga actcagccac gagtgtgata tggtcattgg ctcgcgctat gttaaaggcg
     4261 gtggcacttt gaactgcccg tggaaacggc gcatgctcag ccagggtgca aatgccttca
     4321 ccaaattcat gctgagccag cgcgcgcgtg attgcaccgc cggtttccgt tgttatcggc
     4381 gcgaagtgct ggagtcgatc gatctggatt cgattttttc aaacggctat tcgttcttga
     4441 ttgaaatgtt gtactacgtg cagcggcgcg gttggcagat tggcgaagtg ccgatcgtgt
     4501 tcgaagatcg gcagcgcggc aaatcgaaaa tttctcagaa cgaagtgtcg cgcgcgctgc
     4561 aaacggtcct ccgcttggaa atgcggcgca tgcgtggcat gtttcaacgc gaggcacgtt
     4621 aatggaatac gttcgttgta atttgtgcgg acaggataaa acgatcccgc gttttcccag
     4681 caccgtcaaa ccggatactg tgccgcagaa tgtcgaagcg tttcgctgca ccagtccggg
     4741 ctatggccgt cactttgcga tcgtgagctg ccagaactgc gggctggtgt acaccaatcc
     4801 gcgcttggat ggaaaacaga tcatcgattc gtacgtagcg gtcgaagatc cgttgtatct
     4861 tgaagagcgc gatggccggg tgttgacttt cgagcggcat ctcaaaccat tggagaagct
     4921 acatgcgccc cccggcaagt tgttggatgt cggtgcttat ac
//
LOCUS       DASRTJ010000358         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_248966,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000358 DASRTJ010000000
VERSION     DASRTJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248966"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VFF70_07285"
     CDS             <1..1270
                     /locus_tag="VFF70_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY44536.1"
                     /translation="ESIIAPGTIPGDAFLGTLRGDANHLNWSFFQDKILANFTGGEPI
                     LPYAFLILWATGAWTGLRRARRSTVLLLVWAILPAAVVIVFLRHRGTFFAIRYILYTL
                     PAFLLLIAYSVTSGVRALYRTRSAPLRPNLWRFMQLTASIGLIVLAGHLVNASLTYLI
                     GHREHWREAGQFLMENVQPSDLLVLSQAGELIEFYAPTLPVTPIRSNESKDLPMPAPA
                     QRMWLVLNVYNYPPGMYEAWLYSRLHVEYRFDDALQVELITTADDQAELLREAADVRV
                     PPRPMVWAGLARQNESAGNIDTAFDQMQVALTLTPDRIELPDLLLQQADMFRRAQRWG
                     AAIADYQQVLNLGARQVMALVGLGRVYLAQNQIETARAQFKQALAIDPDSYEANLFMA
                     QSFTRTGDQAQAAAYYGVAEKLNGDMPTPP"
     gene            1270..>2921
                     /locus_tag="VFF70_07290"
     CDS             1270..>2921
                     /locus_tag="VFF70_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44537.1"
                     /translation="MNDTPITYHASRRTFLATLVRARWLVLIVSLIIILLPVPYVLSN
                     WTPVGDEPHYLLAAYSLVHDGDLDLANNYAQGDYRAFYDLPIDPHVRIGADGRLYLSH
                     DIGLPILIAPAYAFGGRAAVIIFLAIIAALVAVNVYWLAYEVTGHPVAAMLTWLILLL
                     TPILSVYAYLVYPEMIGALIVIWTTRRLFKIDASRVTRHSSLITQSAIVALALAVLPW
                     LSARFIPIAVFLLAWKIRRDRSNRKQILISIGVMIGSLIGYFALNAWLSGGAASAIDF
                     DTGLIAIGFQGISIERILRGLIGWWLDQQRGVLIYSPVDIIALIGLPWLWRRLRWSGI
                     ALLAPLLVAYLSAVAWGGFWVGWEISARYLVIGLPLLAAPLALALVHIRRVAFRGLVI
                     VTFAIALINSVVLIKSPGLWGYRESIVLFYDRYTSIDLWRWLPAMSAGAAIDPTVGAI
                     DAAEVVSDNNRSAWHTPIGSGGVVIQTSSLTDVTIGIYELHFDARAENIPAVDAPLLS
                     IDVFSAEGVSLKHQLLSGADFAADGSYRSFSVAFDQPFYNKWT"
BASE COUNT          547 a          817 c          814 g          743 t
ORIGIN      
        1 tgaatcgatc atcgcacccg gcaccattcc cggcgatgcg tttctgggca cgctgcgcgg
       61 cgacgcgaat cacctcaatt ggagtttctt tcaggataaa atcctggcga acttcaccgg
      121 cggcgaaccg atcttgcctt atgcgttttt gatcttgtgg gcgaccggcg catggacggg
      181 actgcgtcgg gcgcgccgat cgactgtgct gctgctcgtc tgggcgatct tgccagccgc
      241 cgtggtgatt gtctttctga gacatcgcgg cacattcttt gccatccgct acattttgta
      301 cacgctaccg gccttcttac tgttgattgc ctatagcgtg acgagtggcg tgcgtgcgtt
      361 gtatcgaaca cgctccgctc cacttcggcc aaacctttgg cgattcatgc agctcaccgc
      421 ctcgatcggg ttgatcgtcc tcgccggaca tttggttaat gcctcactca cttatctcat
      481 cggccatcgc gaacactggc gcgaggcggg acaattctta atggaaaatg tgcaaccgag
      541 cgatctcttg gtcctgtcgc aagcgggtga actgatcgag ttctatgcgc caaccctgcc
      601 ggtaacacct atacgttcta atgaatccaa agatttgccc atgcccgcgc ccgctcagcg
      661 aatgtggctg gtgctgaacg tatacaatta tccgccgggc atgtatgaag catggctcta
      721 cagccgcctg catgttgaat atcgcttcga tgatgcattg caagtggagt tgatcacgac
      781 ggcggatgat caagccgaat tgttgcgtga agccgccgat gtacgcgtgc cgccacggcc
      841 gatggtttgg gccggactgg caaggcaaaa tgaatcggct ggcaacattg ataccgcgtt
      901 cgatcaaatg caggtcgcgc tgacgctgac ccccgatcga attgagctgc ccgacttgct
      961 gttgcaacag gctgacatgt tccgccgggc tcaacgctgg ggggccgcca ttgcagacta
     1021 tcaacaagtg ctgaacctgg gtgcgcgtca agtcatggct ttagtcggat tggggcgggt
     1081 gtatttggcg cagaatcaaa tcgagacagc ccgcgcgcag ttcaagcaag ccctggcgat
     1141 cgatcccgat tcctacgagg ccaatttgtt tatggcgcaa agctttaccc gcacaggcga
     1201 tcaagctcaa gccgctgcat attatggggt cgcagaaaag ctcaacggcg atatgcccac
     1261 accaccctga tgaacgatac accgatcacc tatcacgcct cccgccgcac ctttctggca
     1321 acgctcgtcc gagctcgctg gcttgtgctg attgtgtcat tgatcatcat actgctgccc
     1381 gtgccgtacg ttctatcgaa ctggacgccg gtcggcgacg agccgcacta cttgctcgcc
     1441 gcttatagtt tggttcatga tggcgatctc gatctcgcca acaattacgc gcaaggcgat
     1501 taccgcgcct tctacgatct gccgattgat ccacacgtgc gcattggcgc ggatggccga
     1561 ttgtatttgt cacacgatat cggcctgccg atcttgatcg cgccggcgta tgcattcggc
     1621 ggacgcgccg ctgtcatcat cttcttggcg atcattgccg cgctcgtcgc agtgaatgtt
     1681 tattggctcg cgtatgaagt caccggccat ccagtcgccg cgatgttgac ctggctgata
     1741 ctgctcttaa cgccgatatt gtcggtgtat gcttacttag tgtatcccga aatgatcggc
     1801 gcgctcatcg tcatttggac aacacggcga ctcttcaaga ttgatgcatc acgcgtcact
     1861 cgtcactcgt cacttatcac acaatccgcg attgtcgcgc ttgcacttgc cgtcctgccc
     1921 tggctctctg cccgctttat tccgattgcg gtgttcttgt tagcgtggaa aataaggcgc
     1981 gatcgatcga accgcaaaca gatattaatc tcgatcggcg tgatgatcgg ctcgctgatt
     2041 ggttacttcg cgctgaatgc gtggctaagt ggtggagccg caagcgcgat cgattttgat
     2101 acggggttga tcgcgatcgg ttttcagggt atctcaatcg aacgcattct gcgcggcttg
     2161 attggctggt ggctcgatca acaacgcggc gtgttgatct actcgccggt agacatcatc
     2221 gcgttgatcg gcttgccgtg gttgtggcgt cgcctgcgtt ggtcaggcat tgcgctgtta
     2281 gcaccgttat tggtcgcgta tctgtccgcc gttgcgtggg gcggcttctg ggtcggctgg
     2341 gaaatttccg cacgctatct cgtgatcggc ttgccgttgc tggccgcacc gttggcttta
     2401 gctttggtgc atattcgtcg cgtagccttt cgcggactgg tcatcgttac attcgcgatc
     2461 gcgctgataa actcggtcgt actgatcaaa tctccgggcc tgtggggtta ccgtgaatcg
     2521 atcgtgttat tctatgatcg ctacacctcg atcgatctct ggcggtggct gcctgccatg
     2581 agcgccggag cggcgatcga tccaactgtc ggtgcgatcg atgcggccga agtggtgagt
     2641 gacaacaacc gatcggcctg gcataccccg atcggttccg gcggcgtcgt gatccaaacg
     2701 tccagtttga cggatgtcac catcggtatt tacgaacttc attttgatgc gcgtgccgaa
     2761 aatattccgg cagttgacgc gccgctgtta tcgatcgatg tcttttcggc cgaaggcgtt
     2821 agcttaaagc atcaattact gagcggcgct gactttgccg cggatggcag ttatcggtcg
     2881 tttagcgtcg cgtttgatca accgttttat aacaaatgga c
//
LOCUS       DASRTJ010000359         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_198449,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000359 DASRTJ010000000
VERSION     DASRTJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198449"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2106)
                     /locus_tag="VFF70_07295"
     CDS             complement(<1..2106)
                     /locus_tag="VFF70_07295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZY44538.1"
                     /translation="MSISKKRLGVLTSGGDAAGMNAAVRSVVRTALDQGLEVFAIYEG
                     YQGLVDGGTRIHAMQWDSVGGILNRGGTVIGTARCEAFRTHAGRLQAARHLLEHEIDS
                     LVVIGGDGSLTGANIFRQEWPTLLQELIQLGQINQDIADRHAHLTIVGLVGSIDNDMF
                     GTDMTIGADTALHRITEAVDAISSTAASHQRTFVVKVMGRNCGYLALMAGLATGAEWI
                     LIPEHPPADNWAEKMCADIRAGRTAGRRDSIVIIAEGARDISGNPIGSTQVQQALEKC
                     LGEEVRVTVLGHVQRGGAPSAFDRNLGTLMGHEAVRAVLDAMPQTEPQMICLRGNRIT
                     RAPLMKCVEQSNAVLDVIAAHHYEQAMQLRGSSFREAFDTVKTLLRSLPHEPQPGHKR
                     LRLAVMNCGSPAPGMNTAVRAAVRIGLDRGHSMIGVLHGFKGLIEGQIKEVDWMSVNG
                     WASLGGSELGTNREIPSGSDLYAIARNIEKYEIDGLLIIGGWAGYKGAHELFRERENY
                     PAFNIPIICLPATIDNNLPGSELSIGADTALNTIVVSVDKIKQSGVALRRCYVVEVMG
                     RHCGYLALMGGLATGAERVYLHEEGVGLRDLQNDLEQLIEGFKHGKRLGLLIRNEQAH
                     AVYTTSVMCAVFEAESGGLFDVRQAILGHMQQGGDPSPFDRIQATRLAVRCIDFLIEN
                     ASNQTPAGAFIGLEAGGIVI"
     gene            complement(2441..2869)
                     /locus_tag="VFF70_07300"
     CDS             complement(2441..2869)
                     /locus_tag="VFF70_07300"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HZY44539.1"
                     /translation="MNRKLKLSVLVLAAIVLPLLGACGGGAPANPGLINVTVETYKIT
                     LSANSTPAGKVTFHITNKASDQVHELVVIQTDLAADKLPLDADGNVDEDKITGMGEVE
                     DVAIGASVDLALDLPAGHYVLICNIATHYHEGMHVDFTVK"
BASE COUNT          611 a          856 c          809 g          687 t
ORIGIN      
        1 gatcacgatg ccgcccgctt cgagtccgat gaacgcgccc gccggcgttt ggttcgaggc
       61 attctcgatc agaaaatcga tgcagcgcac cgccaagcgg gtggcttgaa tccgatcgaa
      121 cggcgacgga tcgccgcctt gctgcatgtg ccccaagatc gcttgacgca catcgaataa
      181 gccgccgctt tcggcttcaa acacggcgca cattacgctg gtggtataaa cagcatgcgc
      241 ttgttcgttg cggatgagca gccccaagcg tttaccgtgc ttgaagcctt caataagttg
      301 ttcgagatcg ttttgcagat cacgtaagcc cacgccttct tcatgcagat acacacgttc
      361 cgcgccggtc gccagcccac ccatgagcgc caggtatccg cagtggcggc ccatcacttc
      421 gacgacgtag cagcggcgca gcgctacacc cgattgtttg atcttatcga ccgagaccac
      481 gatcgtgttt aaggcggtat ccgcgccgat gctcagttcg gagcccggca agttgttatc
      541 gatcgtggcg ggcagacaaa tgatcgggat gttgaaagcc gggtagtttt cgcgctcacg
      601 aaatagttca tgtgcgcctt tgtagcccgc ccagccgccg atgatcaaca agccatcgat
      661 ctcatacttc tcgatgttgc gggcgatggc atagagatcg ctgccgctcg gaatttcgcg
      721 attggtgccc agttccgatc cgccgagtga agcccaaccg ttgacactca tccaatcgac
      781 ttctttgatc tggccttcga tcagaccttt gaagccgtgc agtacgccga tcatactgtg
      841 gccccgatcg agtccgatgc gcacggcggc acgcacggcg gtattcatgc ccggcgcggg
      901 cgagccgcaa ttcatcacgg ccagacgcaa ccgcttgtgg ccgggttgtg gttcgtgcgg
      961 cagagaccgc agcaatgttt tcacagtgtc gaatgcttct ctaaagctgc tgccccgcag
     1021 ctgcatagcc tgttcgtaat ggtgagcggc gatcacatcg agcacggcgt tggattgctc
     1081 gacgcacttc atgagcggtg cgcgagtaat gcgattgccg cgcaagcaga tcatctgcgg
     1141 ttcggtttgc ggcatggcgt ccagcaccgc ccgcacggcc tcgtgcccca tcaacgtgcc
     1201 gaggtttcga tcgaaggcgc tgggtgcgcc gccgcgctgc acatgaccga ggacggtgac
     1261 gcgtacttct tcacctaagc atttttccag cgcttgttga acttgcgtgc tgccgatcgg
     1321 attcccactg atgtcgcgcg cgccttcggc gataatcaca atactgtcgc gccgtcccgc
     1381 cgtgcggcca gcgcgtatgt cggcgcacat tttttcagcc caattatccg cgggcggatg
     1441 ttcgggaatc agaatccatt cggcgccggt ggccagcccg gccatcaagg ccaggtagcc
     1501 acaattgcga cccatgactt tgacgacgaa ggtgcgttga tgactggcgg ccgtgctgct
     1561 gatggcgtcg actgcttcgg tgatgcgatg cagcgccgtg tccgcaccga tggtcatgtc
     1621 agtgccgaac atatcattgt cgatcgagcc caccagcccg acgatggtca agtgcgcgtg
     1681 ccgatcggca atgtcctgat tgatctgtcc caattggatc aattcttgca acagcgtcgg
     1741 ccactcctgc cgaaaaatat tcgcgccggt caagctgcca tcgccgccaa tcaccaccag
     1801 gctgtcgatc tcatgttcta gcaaatggcg cgcggcttga agccgtccgg catgtgttct
     1861 gaacgcttcg cagcgggctg tgccgatcac cgttccgccg cgattcaaga tgccgccgac
     1921 ggaatcccac tgcatagcgt ggatgcgtgt gccgccatcg accagacctt gatagccttc
     1981 gtagatcgca aagacctcca gcccttgatc gagtgccgtg cgcaccaccg aacggaccgc
     2041 cgcgttcatg ccggccgcat cgccgccgct ggtcaatacc cctaaacgtt ttttagatat
     2101 actcataaca ctttctctaa ccactccaat ttttgaacct gttaatccga gcagaattct
     2161 actccatcct gttcgattgg cctagtgtca gggcagagta agattcaatg cggaatgtca
     2221 tattgttgac gtggcgaaag gagttggagt ttgtggaggc ttgtcgtttg attgacaatc
     2281 tcaaacttcg aattcaaaat cgatgaagaa gaccgggttt gctgatgagt gcttcgtctc
     2341 gagtgcggca gaagtgcaaa tgggatttgc gtatataaaa gatcagcggg ctgatcgata
     2401 gcgatcagcc cgctgttaac ttcagaataa agagtctcgc ttactttacg gtgaagtcaa
     2461 cgtgcatgcc ttcatgataa tgagtagcaa tgttgcaaat caacacgtag tggccggcgg
     2521 gcaggtccaa ggctaaatca actgatgcgc cgatggctac atcttcaacc tcgcccatac
     2581 cggtgatctt gtcctcatcc acattgccgt cagcgtccag cggcagtttg tcggcggcta
     2641 aatccgtttg gatgacgact agttcgtgca cctgatcgct ggccttgttg gtgatgtgaa
     2701 aagtcacctt gccggccggg gtcgaattag ccgacaacgt aattttatag gtttcgaccg
     2761 taacgttgat caagcccggg ttcgccggcg ctccgccccc acaggcccct aacaaaggta
     2821 gaacgattgc ggctagcact aacactgaga gtttcaattt gcggttcatt taaagatctc
     2881 cttttaacta ggctggcatt atcttagcac atattttcat catgtccatc cgcttggatt
     2941 gttgcttgcc tgcacagcgg ggc
//
LOCUS       DASRTJ010000360         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_324702,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000360 DASRTJ010000000
VERSION     DASRTJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324702"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFF70_07305"
     CDS             <1..397
                     /locus_tag="VFF70_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44540.1"
                     /translation="FNYYYVYYRTLAGGDTTSKLQETAEAEYLWDNPQILRLLLVLYR
                     FIYGWQDALLGKIDRAITPDQAAETYRDKRILTAITVMGLGFQLLLIALLAWINRSTW
                     TLGLFIGPMNVYWVIIIFIRYRLSHRRGT"
     gene            404..1036
                     /gene="folE"
                     /locus_tag="VFF70_07310"
     CDS             404..1036
                     /gene="folE"
                     /locus_tag="VFF70_07310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZY44541.1"
                     /translation="MHEEDSVYAAAQQHQHPLSDHSREKVEEAVRAILTNVGEDPDRD
                     GLLHTPERVARMYEELLDGYHIDPIATINHALFDVDYDEMVVVKDIEYYSLCEHHLVP
                     FYGRAHIAYLPKGKVLGLSKIPRVVDMFARRLQVQERMTKQIADFLTEVLHPLGVAVV
                     VEGAHLCSMMRGVKKANARMVTSALTGRFKSDARTRAEFFNHIRRNPADE"
     gene            complement(1219..1974)
                     /locus_tag="VFF70_07315"
     CDS             complement(1219..1974)
                     /locus_tag="VFF70_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY44542.1"
                     /translation="MDLELGGKVVLITGASGGLGREMAVDFGREEARVVVHYRSGAEA
                     AELVAQTIEGMGGEALTASADLSDRSSIQKMISEVAAEWGGIDVVINGAVSFEGGVPI
                     EQLTIDQWDKMIATTLTGTYHLISLVLSHMRQEKWGRIVNIASRTGLVGGAKLAHYSA
                     AKAGVIGLTRALAKELGPDGILVNAIAPTTILTPKEREKMTPDRLEKLIAQIPLRRIA
                     TPDDISRVALFLGSGMNTFVNGEVITVGGGAQT"
     gene            2150..3058
                     /locus_tag="VFF70_07320"
     CDS             2150..3058
                     /locus_tag="VFF70_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44543.1"
                     /translation="MEESSKDRLRRILGGGSSNTAVKRVTPRERPDQNELKLNDALRP
                     ATPNTSNLFTGPPGLTINPLPPIAPAQPIEQPAIPKFDPRITQPVQAPAAPSSSDVRA
                     HLQELRSKMAQLSNEFATGEINRMQFEAIYKHYQEKLQQVENQMYALPGSDAWQGAIT
                     QGMTGRLRERHRADVLSYAIYDNESSMPLATVGTFNIDTSLLVPMLSSFRSATTEIFG
                     AGLRSTEIEGGRWLCFMPGHFTTLIALFSIEPAKLQLSLIEDLHRDFETANVPAFEQG
                     QADEAAQSFTRIWALDSFSAPSTEME"
     gene            complement(3125..3205)
                     /locus_tag="VFF70_07325"
     tRNA            complement(3125..3205)
                     /locus_tag="VFF70_07325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3169..3171),aa:Leu,seq:gag)
BASE COUNT          783 a          894 c          843 g          742 t
ORIGIN      
        1 cttcaattac tattacgtgt attatcgaac tctagcgggt ggcgatacca ccagcaagtt
       61 gcaggaaacc gctgaggccg aatatctgtg ggacaatcca cagatactgc ggcttctgct
      121 ggtcttgtat cgattcattt acggctggca ggatgcgctg cttggcaaga tcgatcgagc
      181 catcacgccc gatcaggccg ccgaaaccta tcgagataaa cgaattctga ctgccataac
      241 cgtgatgggt ctcggtttcc aattgctgtt gatcgcgttg ctggcatgga tcaatcgatc
      301 gacttggacg ctggggttat tcatcggtcc aatgaatgtg tattgggtta taatcatttt
      361 tatacgttat cggttatcac atagaagggg aacatgatgc gttatgcatg aagaagattc
      421 cgtttacgcc gccgcgcaac aacatcaaca tccattgagc gatcactcgc gcgaaaaagt
      481 ggaagaggcg gtgcgggcaa ttttaaccaa cgtcggcgaa gatccggatc gagacggctt
      541 gctgcacacg cctgaacgcg tcgcccggat gtatgaagaa ctgctggacg gctatcacat
      601 cgatccgatc gcgacgatca atcacgcgtt gttcgatgtg gactatgatg agatggtcgt
      661 ggtgaaggac attgagtatt acagtttatg cgaacatcat ttggtgccgt tttatgggcg
      721 ggcgcacatc gcttatttgc cgaaaggcaa agtgttgggg ctgagcaaaa ttccgcgcgt
      781 ggtggatatg ttcgcgcgcc gattgcaagt ccaagaacgc atgacgaagc agatcgccga
      841 cttcttaact gaagtgctgc atccgctcgg tgtggcggtc gtcgtggaag gtgcgcatct
      901 atgttcgatg atgcgcggcg tgaagaaagc gaatgcacga atggtgacca gcgcgctgac
      961 cggccgcttc aaatcggatg cgaggacccg cgccgagttt ttcaatcata ttcgacgcaa
     1021 tccagcggat gaatgagaga cggcgagaac aaaagaggac gcgctaaaca atgcgtgcgt
     1081 cctcttattt gttcgtgtcg attcgtagat aaaaatctat agatgataca ttcatccttg
     1141 cgagagtgta tacagctggc gatcgatctt caagcccaac cctcgcaagg tcttccatcc
     1201 cttacttctt gcctctggct aagtctgtgc accgcccccg accgtgatca cttcgccatt
     1261 cacgaatgta ttcataccgg aacccaagaa caatgcgaca cgtgaaatat catcgggcgt
     1321 agcgatgcgc cgcagcggaa tctgagcgat caacttctcc aagcgatccg gcgtcatctt
     1381 ctcgcgttcc ttcggcgtga ggatcgtcgt cggcgcaatc gcattcacca agattccatc
     1441 cgggccaagt tctttcgcta atgctctcgt caaaccaatc acgcccgcct tggccgcgct
     1501 gtaatgcgcc agcttcgcgc cgccgaccaa gcccgtgcgc gatgcgatgt tgacgatccg
     1561 tccccatttt tcctgccgca tatgcgataa caccaaactg atcaagtgat acgtaccggt
     1621 caaagtggtg gcgatcattt tgtcccactg atcgatcgtc aactgctcga tgggcacgcc
     1681 gccttcaaaa gagaccgcgc cgttgatcac cacatcgatg ccaccccact cggccgccac
     1741 ttcgctgatc atcttttgga tcgaagagcg atcgctgaga tcagcggaag ccgtcagcgc
     1801 ttcgccgccc atgccctcga tcgtctgcgc gaccaattcg gcagcctccg cacccgatcg
     1861 ataatgcacc acgacgcgag cttcctcgcg gccaaaatcg acggccatct cgcggcccaa
     1921 gccaccactc gcgcctgtga tcagaacaac tttaccacct agttcaaggt ccatcgcgat
     1981 cgctcctgac tcagctcgat ctcatactta acagcttcgt ctgtaaatac tgcgattata
     2041 gacgattcaa cgctcaagtc aaaatttttc aagtcgcaat tctattgact cgctcccaaa
     2101 tcatgctaca atcgattcag cttaccattg aaattgttga tgattggata tggaagagtc
     2161 ttcaaaggat cggctgcgga gaatattggg cggcggctcg agcaacactg ctgtcaagcg
     2221 tgtgactccg cgtgagcgcc ctgatcagaa cgaactgaag ttgaacgacg cgttgcgacc
     2281 tgcaacgccg aacacctcga atttgttcac cgggccacca ggtctgacga tcaatcccct
     2341 accaccgatc gcgccagctc agccgatcga gcaaccagcg attccaaaat tcgatccgag
     2401 aatcacgcag cccgttcaag cgccagccgc gccatcgtcc agtgatgtgc gtgcgcactt
     2461 gcaagaacta cgcagtaaaa tggctcaatt gagcaacgag ttcgccacgg gtgagatcaa
     2521 tcgcatgcaa ttcgaagcga tctacaaaca ttatcaagag aaattacagc aggtcgaaaa
     2581 tcaaatgtac gccctgcccg gatcggatgc gtggcaaggc gcgatcactc aaggcatgac
     2641 gggccgcttg cgcgaacgac acagagcgga cgtcttgagc tacgcgatct acgacaatga
     2701 atcgagcatg ccgttggcca ccgtcggcac cttcaatatt gatacgagcc tgctggtgcc
     2761 gatgctctct tcattcagat cggcgacaac tgaaattttc ggcgcgggct tgcgcagcac
     2821 agagatcgaa ggtgggcgtt ggctgtgctt tatgccggga catttcacga cattgatcgc
     2881 cttgttctcg atcgaaccgg cgaaattgca gttgagtttg atcgaagatt tgcatcgtga
     2941 tttcgaaacg gcaaatgtgc ccgcgtttga acagggccaa gccgatgaag cggcgcagtc
     3001 gttcacgcgc atttgggcgt tggactcttt ctcagcccct tcgactgaga tggagtagga
     3061 atttccagct gcatcaaaat aataaaagcg gcacatggcg aagatgtgcc gctttattgg
     3121 tgtgtgcccg cgagaggact cgaacctcca cgccttgcgg cacaggccct caacctgctg
     3181 cgtatgccag ttccgccacg cgggcaatat ccaacggcaa ttatatctgg attgaagtga
     3241 ttgtcaacgc atgcatgaga tc
//
LOCUS       DASRTJ010000361         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_324782,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000361 DASRTJ010000000
VERSION     DASRTJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324782"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFF70_07330"
     CDS             complement(<1..1187)
                     /locus_tag="VFF70_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZY44544.1"
                     /translation="MASSAPLDWPYRFSRRAISARRSVMRELMPLTTRPDIISFAGGL
                     PAPDLFPVEDYQACIDHVMTTEGKRAMQYGPPFAPLKESIVHLMKLRGVEAKADEVFI
                     TNGAQQGLSIVSQLLLNPDSPIIVEQIAFTGIGQVIAERNAMPLTVGTDLDRGIDVDN
                     VEYLLKLTGQVRAIIEVPDFHNPLGVSLAAENRTRLVDLSFQYRTPIIEDDPYGLLRY
                     EGENIPPLKAHDTDGGIIYLGSFSKILAPGLRIGWMIVPQAALGKLTVIKESQDLETS
                     QLTQRTIAEFIQRGLFDAHLQKLKAAYAIRRTAMLDALDREFPRAAKWSKPNGGIFIW
                     VTLPEGVSTLELLPRAVEEEKVAFIPGSAFGINGGGESSMRLNFSNATPESIEAGIKR
                     LGK"
     gene            1478..2425
                     /locus_tag="VFF70_07335"
     CDS             1478..2425
                     /locus_tag="VFF70_07335"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZY44545.1"
                     /translation="MTQFAFFKGEIVPIDQAKVSIMTHALNYGTGCFEGIRAYWNAKE
                     EQLFIFRLYEHYQRFIASQKIMMMEPRVSAKQLSDITIELLQKENYHTDSYIRPLAYK
                     TDEIIGVKLHGLNDDVSIWTTPFGRYVDNEEGASVGFSSWRRVSDNNVPPRGKITGAY
                     VNSAFIKTEAVLNGFDEALVLNDRGYVCEGSAENVFLVRDGVLITPPVQEDILEGITR
                     STIMELAGEELGLQVLERPISRTEFYVAEEAFFVGTGVQVTAIATVDHRPIGTGQMGP
                     IVHAVRDMYFDVVRGRVPKYRHWCTPVYVTENAPVLAFK"
     gene            2543..3436
                     /locus_tag="VFF70_07340"
     CDS             2543..3436
                     /locus_tag="VFF70_07340"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZY44546.1"
                     /translation="MEWITDRRIGSQTGVLFIIAIVMIDIILIGLILTQPIGLMTFIA
                     GLLAIFSLPLIGLIGFQLRGLSRSGYMLDRNALTIFWGPIKQIVPTAAIQRIMLGSEV
                     EGPIKFRGWRWPGLIVGQGEVPDAGLTLFYATTALAEQLIVITPLLSYAISPTDVAGF
                     IESIKARYELGPTQELEQTSVRPPIFDAPLWRDRWMYGLETAGFVLCAAMFAYVCIRY
                     AGLPQKLPLHYTAAGLVDRFGPPSQSFILPFIGLLALVGNSAIGLWLYGREKMAAYVL
                     WGGMIFVQVLLWVGSINLLKL"
     gene            3580..3792
                     /locus_tag="VFF70_07345"
     CDS             3580..3792
                     /locus_tag="VFF70_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44547.1"
                     /translation="MSDTDTKRRPIVPEEFASHAKAAAHEARHAFEALMPKMPERFVE
                     HRRAARREALMAMRSLIDAAIERTNR"
     gene            complement(3816..>4197)
                     /locus_tag="VFF70_07350"
     CDS             complement(3816..>4197)
                     /locus_tag="VFF70_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44548.1"
                     /translation="FNVFASAVPFAEKMIAAESGSIFRTVIDESIEIGKALVRLPGQI
                     DHLTDVLLRGELRFTVNDTDKLIKEMHNMNRSMIRLQWTLISLGLLFTSIVLDVGGYK
                     NVSPIVLAAAIVAGVWFFVRGVLQ"
BASE COUNT          945 a         1144 c         1140 g          968 t
ORIGIN      
        1 atcttgccca gccgcttgat cccggcctcg atcgattccg gcgtggcgtt gctgaagttc
       61 aagcgcatcg aactttctcc gccgccgttg atgccgaatg cggagccggg aatgaacgca
      121 actttttctt cttcaactgc tcgcggcagc aactctaagg tagagacacc ctcaggcagc
      181 gtgacccaaa tgaagatgcc cccattaggc ttcgaccatt ttgctgcgcg cggaaactct
      241 cgatcgagcg cgtcgagcat cgctgtgcgt cgaatggcat acgcggcttt gagtttttgc
      301 aggtgcgcat cgaacaaacc gcgttgaata aattcggcaa tggtgcgctg cgtgagctgt
      361 gatgtttcca aatcttgcga ctctttaatc acagtcagtt tgccgagtgc cgcttgcggc
      421 acaatcatcc agccgatccg taaacctggc gcgagaatct tcgagaacga tccaagataa
      481 ataatgccac catccgtatc atgcgcttta agcggcggga tgttctcgcc ttcatagcgt
      541 aagagtccat agggatcatc ttcgatgatg ggtgtgcggt attggaaaga gagatcaacc
      601 aatcgggttc tgttttccgc tgcaaggctg acgccgagcg gattgtggaa gtccggcact
      661 tcgatgatcg cgcgcacttg gccggtcaac ttcagcaagt attcgacgtt atcgacatcg
      721 atccctcgat ccagatcagt tccgactgtt aagggcatcg cgttgcgttc agcgatcact
      781 tggccgatac ctgtgaacgc aatctgctcg acaatgatcg gcgaatcggg attgagcagt
      841 aattggctga caatgctaag gccttgctgc gcgccgttgg taatgaagac ttcatcggct
      901 ttcgcttcga caccacgcaa cttcatcaga tgcacgatcg attctttgag cggtgcgaac
      961 ggcggaccgt actgcatggc gcgtttgcct tcggtcgtca tcacatgatc gatgcaggct
     1021 tgataatctt caacgggaaa cagatcgggc gcgggcagcc cgcccgcaaa ggagataata
     1081 tccggccgcg tggtgagcgg catcaactca cgcatcaccg atcgacgcgc gctgatggca
     1141 cggcgggaaa aacggtaggg ccagtcgaga ggggctgatg acgccatggg aatattatcg
     1201 cactgagtct gagtagggtc aaacatcttc tgggggcggc gcattcccgc atggattcag
     1261 agtgatctac ctcaccttca cgcctaagcg gccaatcgat cgcaatgcat tggcgaatgt
     1321 gtcgcccctg cccatgtgaa aagatcgaca agaccatcga ttagatcatt gacactgacc
     1381 tcaaacgatc ctataatctg atcgaacccc cacaccgttg agcagctaat accgatcggg
     1441 cttgggggta attctttggc accgggaggc taaccccttg acccagttcg cattcttcaa
     1501 aggcgagatc gttcccatcg atcaagcgaa agtcagcatc atgacgcacg ccctgaatta
     1561 tggcaccggc tgttttgaag gcattcgggc gtattggaat gccaaagaag aacagctgtt
     1621 tatcttccgt ttgtacgagc actatcaacg cttcatcgcc tcgcaaaaaa ttatgatgat
     1681 ggagccgcgt gtctcggcca agcagctcag cgacatcacg atcgagctgc tgcaaaaaga
     1741 aaactatcac accgattctt acatccgccc gttagcctac aagaccgatg agatcatcgg
     1801 cgtcaagctg cacggcctca acgatgatgt cagcatctgg acaacgccct ttggccgcta
     1861 cgtcgacaac gaagaaggcg cgagcgtggg cttctcatcg tggcggcggg ttagcgacaa
     1921 caacgttccg ccacgcggca agatcaccgg cgcgtatgtg aactcggcct tcatcaagac
     1981 tgaagccgtt ctcaacggct tcgatgaagc gttggtgctg aatgatcgcg gctatgtgtg
     2041 cgaaggcagc gccgaaaatg tctttctcgt tcgcgacgga gttttgatta cgcctccggt
     2101 ccaggaagat atcctcgaag gtatcacccg ctcgacgatc atggaactgg ccggcgaaga
     2161 gttgggtctg caagttttgg agcgtcccat cagccgtacc gaattctacg tggctgaaga
     2221 ggcgtttttt gtggggacgg gtgtgcaagt caccgccatc gccacggtcg atcatcggcc
     2281 gatcggtaca ggccagatgg gccccatcgt ccatgccgtc cgcgacatgt atttcgatgt
     2341 ggtgcgcggc cgcgtgccaa aatatcggca ttggtgcacg ccggtgtatg tgacagagaa
     2401 cgcgcccgtg ctcgctttca aataacgcgg tctttcgctt aacagataaa aagaaaccga
     2461 gtacaatagt gctcggtttt gtttttgatc gaatgatatc ctctcggagg tttagaatct
     2521 ccgtgaggat cagcgaggtt gtatggaatg gattacagat cggcgcatcg gttcgcagac
     2581 cggagtgctg ttcatcatcg ccatcgtcat gatcgatatc attctgatcg gcttgatcct
     2641 cactcaaccg atcggcttga tgacgttcat cgcgggcttg ctggcgatct tcagcctgcc
     2701 attgatcggc ttgatcggct ttcagctgcg cggcttatcc cgatcgggtt acatgctcga
     2761 tcgaaacgcg ctgacgatct tctgggggcc gatcaagcag atcgtcccta cggccgcgat
     2821 tcaacgcatc atgcttggct cggaagtcga agggccgatc aagtttcgcg gctggcgctg
     2881 gccgggcttg atagtcggtc aaggcgaagt gccggatgcc ggactgactt tgttttatgc
     2941 cacgaccgca ctcgccgaac agctaattgt cattacgccg ttattgtctt acgctatctc
     3001 gccgaccgat gtggccggct tcatcgaatc gatcaaagcc cgttacgaat taggcccgac
     3061 gcaggaactt gagcagactt ccgtgcgccc gccgatcttt gatgcgccgc tgtggcgcga
     3121 tcgatggatg tacggtttgg aaacagccgg ctttgtcttg tgcgcagcca tgttcgcgta
     3181 tgtgtgcatt cgctacgcag gtctaccaca aaaactgccg ttgcactaca cggccgctgg
     3241 tctcgtcgat cgcttcggcc cgccgtcgca atccttcatt ctgccattca ttggcctact
     3301 ggcactcgtc ggcaattcgg cgatcggctt gtggctgtat ggtcgcgaga agatggccgc
     3361 ctatgtttta tggggcggta tgattttcgt gcaagttttg ttgtgggtgg gatcaatcaa
     3421 tttactgaaa ttgtgatcga ttgacaatgg tcttataata gaagttagag tttggatggt
     3481 agacgatgga agatggactc gctcaacgag gaatcaatcc aacttccaac ttccaatttc
     3541 caacttctat aatccaacat ccaatcacag gaggccagta tgtccgacac cgataccaaa
     3601 cgccgaccga tcgttccaga agaatttgct tcgcacgcca aagcggcggc tcacgaagcc
     3661 cgtcacgctt tcgaggcatt aatgccgaag atgccggaaa gatttgtgga gcatcgacgc
     3721 gcggcgcgcc gtgaagcatt gatggcgatg cgcagcttga tcgatgcggc gattgaacgc
     3781 acgaatcgat gagtcagggc gaggtgccct cgccctcatt gcagcacgcc ccgaacaaag
     3841 aaccacacgc cagccacgat cgctgcggcc agcacgatcg ggctgacgtt tttgtaacca
     3901 cccacgtcca acacaatcga cgtaaacaac aagcccaatg aaatcaacgt ccactgcaag
     3961 cggatcatcg atcgattcat gttgtgcatt tctttgatca acttatcggt gtcgttgacg
     4021 gtgaagcgca gttcgccgcg cagcagcaca tcagtcaagt gatcgatctg cccgggcagc
     4081 cgcacgagcg ctttaccgat ctcgatcgat tcatcgatca ccgtccgaaa gatcgatccg
     4141 ctttcggcgg cgatcatctt ctcggcgaac ggcaccgcgg aggcaaacac attgaag
//
LOCUS       DASRTJ010000362         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_97242,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000362 DASRTJ010000000
VERSION     DASRTJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97242"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFF70_07355"
     CDS             <1..323
                     /locus_tag="VFF70_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY44549.1"
                     /translation="YEESASDLGANNWQVFKRVTLPIAGPSLLAGIILTWARALGEFG
                     ATILFAGNFPGRTQTMPLAIYQALETDLGSALAIAAILILIAFVFLLALRFVTHGRVT
                     HPLL"
     gene            complement(384..1205)
                     /locus_tag="VFF70_07360"
     CDS             complement(384..1205)
                     /locus_tag="VFF70_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZY44550.1"
                     /translation="MKVLVCSDGSPAAAQAARFGMEIARGSNEPIELLSVVEHQRDAD
                     KLRSNLEALGHEIETIGAQCNIKIRHGHAAEQILDEAADWQADVIVLGRSGQRGLTRF
                     LMGATAARILQYAACSVLLHKSDRSSLKKILACTAGGEPGLRNVDMAGRVAEHTPAEV
                     TVLHVMSQVALSDKAYLPDLAAAADDLIARETREGKHLKEVIAALTERSIRGVPKVRH
                     GFVVDEVLAELREGNYDLLVIGAHSAHGLTRWLLDDVTADLLEATNLPVLVVRSQ"
     gene            complement(1228..2292)
                     /locus_tag="VFF70_07365"
     CDS             complement(1228..2292)
                     /locus_tag="VFF70_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44551.1"
                     /translation="MSLNTQPHRIHLSKIFFGLLGLFVFLLALQLMRAGARGLAPILK
                     STDLLKTPINSLGFGWLGAYMVLSGSPVAAASLSLFDGNAITELQTFTMINGSRMGAS
                     FIVLFIGFIYMLRGHEPKRSLNMGLLSLLTTWTIYIPVLFIGSTFIANGTFDAVRISA
                     ASKLVPFIDSIFDPIVQWITGWAPGLLIFVVGVGVILLAFNLIDRALPELNLGGTEFD
                     QTPRLLYKPIVMFLLGGAVTTFSLSVSVSLSLLVPLSARGYIRRENVIPYIMGANITT
                     FVDTLVASLFMNHPQAFTVVFVEMATVAIISIIILLTSYRFYERQVLNLANRITASRR
                     NLSLFMLLLVGTPIFLLWIR"
     gene            complement(2398..>2595)
                     /locus_tag="VFF70_07370"
     CDS             complement(2398..>2595)
                     /locus_tag="VFF70_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZY44552.1"
                     /translation="ADYTDPMNGREADPRQHTAVHEYIVKLSKIKDRMQTTTGKQIAQ
                     QRHEFMVKFYAQLDKEIMNNE"
BASE COUNT          611 a          678 c          736 g          570 t
ORIGIN      
        1 agtatgaaga aagcgccagc gatcttggcg cgaacaattg gcaggtcttc aagcgcgtga
       61 cgttgcccat cgccggaccg tcgttgttag cgggaattat tttgacgtgg gcgcgcgcgt
      121 tgggggaatt cggcgcgacg attttgtttg cggggaattt tccgggccgt acccagacga
      181 tgccgctggc gatctatcaa gcgctggaaa ccgatctcgg ttcggcgctg gcgattgcgg
      241 cgattctgat cttaatcgcg tttgtgttct tgctggcgct gcgctttgtt acacatggcc
      301 gcgtgaccca tcccctgttg tgatttggaa aaaaccacgg ataacacgga tttcaatttg
      361 ttatccgtga catccgtagt ccatcattga ctgcgcacaa ccaatacagg caaattcgtt
      421 gcttccaaca aatcggcagt gacgtcatcc agcagccagc gcgttaagcc gtgggcgctg
      481 tgtgcgccga tgaccaagag atcgtagttg ccctcgcgca gttccgctaa aacttcatcg
      541 acgacaaaac cgtggcgcac tttgggaaca ccgcgtatcg accgctcggt gagagcagcg
      601 ataacctctt tgagatgttt gccttcacgt gtctcacgcg cgattagatc atcagccgct
      661 gcggccagat caggcagata agctttatcc gatagagcca cttgcgacat gacgtgcagc
      721 accgtgactt cggccggcgt atgttccgcg acgcggccgg ccatatccac attgcgcaga
      781 cccggctcac cgcccgccgt gcaggccaaa atctttttga gtgaagatcg atcgctcttg
      841 tggagcagca ccgagcacgc cgcgtattgc aagatgcgcg ccgcggtggc gcccatcaag
      901 aagcgcgtca atccgcgctg cccggatcga ccaaggacga tcacatcggc ctgccaatct
      961 gcggcctcat ccaaaatttg ctctgcggcg tggccgtgcc gaatcttgat attgcactgc
     1021 gcgccgatcg tttcgatctc atgccccagc gcttctagat tcgatcggag tttgtcggca
     1081 tcgcgttgat gctcgacaac gctgagcagc tcgatcggtt cgttcgatcc gcgcgcgatc
     1141 tccataccaa agcgcgccgc ttgggcggcg gcgggtgatc catcggaaca cactagaact
     1201 ttcataggtg cttcatcctt ttgaagtcta cctgatccat aaaagaaaga tcggtgtgcc
     1261 gacgagtagc agcatgaaga ggcttagatt gcgccggctg gccgtaattc gattcgctaa
     1321 attcaacact tgccgctcat agaatcgata tgaggtcagc agaatgatga tcgaaatgat
     1381 cgccacagtt gccatttcaa caaatactac agtgaaggct tgtgggtggt tcataaacag
     1441 ggatgcgacc aaagtgtcga caaaggtggt gatgttcgcg cccatgatgt agggaatgac
     1501 attctcacgt cggatatagc cgcgggccga gagcgggacg agcaaactca acgacacgga
     1561 cacagacagg gagaaggtgg ttacggcccc gccgagcaag aacatcacga tcggcttgta
     1621 gagcaagcgg ggggtttgat cgaactctgt cccgccgagg ttcaattcgg gcagagcgcg
     1681 atcgattaag ttgaaggcca ataagatcac tccgacgccg acgacgaaaa tgagcaggcc
     1741 cggcgcccag ccggtgatcc attggacgat cggatcgaag atcgaatcga tgaagggcac
     1801 cagtttgctg gcggcggaga tgcggacggc atcgaacgtg ccatttgcaa tgaaggtact
     1861 accaatgaat agtacgggaa tgtaaattgt ccaagtcgtg agcagcgaaa gcaaacccat
     1921 gttcaggctg cgcttcggct cgtggccgcg cagcatgtag atgaagccaa tgaataatac
     1981 gatgaaggat gcgcccatcc gcgagccgtt gatcatggtg aatgtttgta gttcggtaat
     2041 ggcgttacca tcgaataagc tcaacgaggc ggcggccacc ggcgagccgc tcagcaccat
     2101 gtaagcgccc agccagccaa agcccaaact attgatcggg gttttaagca ggtcggtact
     2161 tttcaaaatc ggtgccagac cgcgcgcgcc ggcgcgcatc aattgcaagg caagcagaaa
     2221 gacgaaaaga ccgaggagac cgaagaatat tttggagaga tgaatgcggt gcggttgagt
     2281 gtttaaactc aaggggctcc tgacaataag atgctgagta acaagtaata acgtattact
     2341 cagtcgccat cgcaatgcgg tacacgtgtc gctcgcactg ccccggcgga cggtaagtta
     2401 ctcattattc attatttcct tgtccaattg tgcatagaac tttaccatga attcatgacg
     2461 ctgctgcgca atctgtttgc cagtggtggt ctgcatgcga tctttgattt tggacaactt
     2521 gacgatgtat tcatgcacgg cagtatgctg acgcggatcg gcttcgcggc cattcatcgg
     2581 atcggtgtaa tcggc
//
LOCUS       DASRTJ010000363         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_22261,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000363 DASRTJ010000000
VERSION     DASRTJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22261"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFF70_07375"
     CDS             complement(<1..362)
                     /locus_tag="VFF70_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007307996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44553.1"
                     /translation="MASVTFNNVAKRYGNVTAVHDLNLEIADKEFLVFVGPSGCGKTT
                     SLRMLAGLEEVTEGKILIGDRDVTDIAPKDRDIAMVFQSYALYPHMSVYDNLAFSLRL
                     RAKTGRVRKLVTLVFNRPA"
     gene            complement(446..1120)
                     /locus_tag="VFF70_07380"
     CDS             complement(446..1120)
                     /locus_tag="VFF70_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44554.1"
                     /translation="MIPVSVFWRALKYLWDELFLLSVIGIMWMLFGLACIVMVPLTLG
                     LSLLPLVPLTVGLFYVTNQVVRGNAIHFQMWFVGARKFLSRSLFWGAVAWSMSLLVWA
                     DLGYYGQISGDIGVAFVTLAVLLALTWIVWQILTLACLIEAGPLSLRAAYRTAWLLML
                     SQPLFVIALIVISGILVIICLYLPVLTGHAFGYVALTANAAVLWAAQKRSGTANRSRE
                     DDPRWQ"
     gene            complement(1260..>2077)
                     /locus_tag="VFF70_07385"
     CDS             complement(1260..>2077)
                     /locus_tag="VFF70_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HZY44555.1"
                     /translation="DLQSIPSAGLAQVVIPDLGTTQMVLSVDDPVGDDHGPGKYTYPT
                     DGVFKPGVFDIKHFEVGYDDKYLAFKITFAGAIPNPWGSPNNLAVQTLDVYVDKDPGA
                     GTGVRKLLPGRNASLPKGDGWDVAAWAEGWTPGVFTPDADGNPKHVDNLDFKVLVDAG
                     QQQVTLRVPLSVFGDNFDPTKAGYTVMVMSQDGFPAPGVWRIRDVDPTASQWRLGGSS
                     GDINHTRIIDLVQPVDATPTQEEALSKYASAVSGDLDQLTPDDFALVPVLTVK"
BASE COUNT          543 a          624 c          476 g          434 t
ORIGIN      
        1 gccggtcgat tgaaaaccag cgtgaccaac ttgcgcacgc ggccggtctt ggcgcgcagt
       61 cgcagcgaga aagccagatt gtcgtacacg ctcatgtgcg gatacaacgc gtacgactgg
      121 aacaccatgg caatatcgcg atccttcggc gcgatgtcgg tgacatcgcg atcgccgatc
      181 aggatttttc cctcggtgac ttcttccaag ccggctaaca tgcgcagact ggtcgttttg
      241 ccgcagccgg atggaccgac gaataccaaa aattctttgt ccgcgatctc caaattcaga
      301 tcatgaaccg ccgtcacatt accatatcgc ttggctacat tattaaaggt cacggatgcc
      361 atactgatct ctccttacaa gattttctcg attggctcac cgggccgatc gatgacacga
      421 tcaacccaaa agtttttcgt tcgatctact gccagcgcgg atcatcttct ctcgatcggt
      481 ttgctgtgcc cgatcgtttt tgcgccgccc ataacaccgc cgcattcgcg gtcagcgcca
      541 catacccaaa agcatgtccg gtcaagaccg gtagatataa gcatattata accaaaattc
      601 cgctgatgac aatcagcgcg atcacaaaca acggctgcga caacatgagc aaccaggccg
      661 tacgataagc ggcgcgcaag ctcaacggcc cggcttcgat caagcacgcc agcgtcagaa
      721 tctgccagac gatccaggtc agagccagca ataccgcgag cgtcacaaat gccacaccga
      781 tatcgccgct aatttgtcca taatagccta aatcagccca aaccagcaaa ctcatcgacc
      841 aggccactgc accccagaat aaactgcggc tcaaaaattt ccgggcgccg acaaaccaca
      901 tctggaaatg aatggcattg ccgcgcacga cttgattcgt cacgtaaaac aaaccaaccg
      961 tcagcggcac cagcggcagt aaagacaagc ccaaggtcag cggcaccatc acgatgcagg
     1021 ccaaaccaaa cagcatccac atgataccga taaccgagag caggaacaat tcgtcccaca
     1081 aatattttaa cgcgcgccaa aagacactga cagggatcat attgagtgat tcgtactcaa
     1141 aaaacgagat gtcattccga gcgaagcgag gaatctctgc ttcgataaac cctcgctgct
     1201 aaccgtcata gagattcctc gccgcgaaaa tgctgcggct cggaatgagg cgctaacatt
     1261 tacttcaccg ttaataccgg caccaaggca aaatcatctg gcgtcagctg atccagatcg
     1321 ccgctaactg cagatgcata tttgctcaac gcttcttctt gtgtcggcgt cgcgtcgacg
     1381 ggctgcacca gatcgatgat gcgcgtatga ttgatgtcgc ccgacgatcc gcctaaacgc
     1441 cattgactcg ccgttggatc gacatcacga atccgccaga caccgggcgc agggaaacca
     1501 tcctgactca tcaccatcac cgtgtaaccc gctttcgtcg gatcgaagtt gtcgccgaac
     1561 acacttagcg gcacgcgcag cgtcacttgt tgttgaccgg catcgactaa gactttgaaa
     1621 tcaaggttat cgacatgttt tggattgcca tcagcatctg gtgtgaaaac acccggcgtc
     1681 caaccttcag cccacgcggc tacatcccaa ccatcacctt tcggcagtga agcattgcga
     1741 ccgggcaata acttgcgcac gcccgtaccc gcacccggat ctttatcgac atacacatcg
     1801 agcgtttgca cggccagatt attcggcgaa ccccacggat ttggaattgc cccggcaaat
     1861 gtgatcttga atgctaaata cttatcatcg taaccgactt caaagtgttt gatgtcgaac
     1921 acgcccggtt tgaacacacc gtccgttgga taagtatatt tgcccggccc gtgatcatcg
     1981 ccgaccggat catcgacgct taagaccatt tgcgttgtgc ccagatcagg tatcaccact
     2041 tgcgccaaac ccgccgatgg gatcgattgc agatcat
//
LOCUS       DASRTJ010000364         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_325072,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000364 DASRTJ010000000
VERSION     DASRTJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325072"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1228)
                     /locus_tag="VFF70_07390"
     CDS             complement(242..1228)
                     /locus_tag="VFF70_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZY44556.1"
                     /translation="MFKDIPEVKAALTQLQYLPSDEIATVLFLAERLDKPILAEGPAG
                     VGKTELAKAWSGATGRELIRLQCYEGLDETKALYEWEYAKQMLYTQLLRDKLSDVLSD
                     AKSLSAAADRLAAEEDVFFSERFLLPRPLLRALTSDKPVVLLIDEIDRADAEFEAFLL
                     EILSDFQISVPELGTIKAKHQPYVLLTSNNTRELSEALKRRCLYLFVDYPSMEQELNI
                     VRMKVPDLNLKLAQQAVQVVHKLRGLDLKKSPSVSETIDWAKALVALNAEHMDEETLN
                     NTLTVLLKHESDVQRARQGMNSRSAAEPTGPIDVDQYNPYGEYRKLPRGRRK"
     gene            complement(1331..2137)
                     /locus_tag="VFF70_07395"
     CDS             complement(1331..2137)
                     /locus_tag="VFF70_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44557.1"
                     /translation="MQISEFQDTQEQTLLCMNYNFAEDCPSNREVALQALGSGVNAKR
                     YWNYNRFFKGLHLLIAYVGTTPVGHIEYLPIEHAPRPVHGQDLTFIDCMYVQPQARGR
                     GTGLALIEAVEEKVRASSSGLATITNAAWPSMSYNLFATHGFITVAEDHGARLMVKAW
                     AKISPPEFLPRRYQPIEPKQSSQTTVDIFWCGQCPHWVQVRDRLTELSHEYGDQITLR
                     GINTDDRSTVERWGISDGVYVNGRLLFASPPSDVDLRIALEQAVKVKVVT"
BASE COUNT          505 a          651 c          587 g          541 t
ORIGIN      
        1 cttcaatgcc cagttgatcg atcgcatgca tggcgtcttc gctttcggcg agactgacgc
       61 gcacgcctga ggcgcgtaag gcgccgatga agtgaacgat gcgggagtcc atatgattaa
      121 tgaaatgtca ttgcgagcga agcgaagcaa tctcccgatc gagtttgaga ttgccacgcc
      181 acctcacttg ctctgtactg catgcgtgca agtatcggcg gctcgcaatg acgcatactc
      241 attatttacg tctgcctcgc ggcaatttcc gatattcccc atacggatta tattgatcga
      301 catcgatcgg acccgttggc tctgccgccg aacggctatt catgccctgt cgagcgcgct
      361 gcacatccga ttcgtgtttc agcaaaacgg tcagtgtatt gttgagcgtt tcttcatcca
      421 tgtgctcggc attcaacgcg accagcgctt tggcccaatc gatcgtttcg ctcacgctgg
      481 ggcttttctt cagatcgagg ccgcgcagtt tgtgcacaac ctgcacggct tgctgcgcca
      541 acttcaaatt gagatcgggt actttcatgc gaacgatgtt gagttcctgt tccatcgacg
      601 gataatcgac gaacaaatac aagcagcgcc gtttcagcgc ctcactcaat tcgcgcgtgt
      661 tgttgctggt caacagcacg tacggctgat gcttggcttt gatcgtgccc agttccggca
      721 cgctgatttg aaagtcactc aggatttcca gcaagaacgc ttcgaattcc gcatcggcgc
      781 gatcgatctc atcgatcagc agcaccaccg gcttatcact cgttagggcg cggagtaacg
      841 gtcggggcaa caaaaatctt tcggagaaga acacatcttc ttcggcggcc aatcgatcgg
      901 ccgcggcact taacgatttt gcatcactca atacatcgga taatttatcg cgcagtagct
      961 gcgtgtacag catctgcttg gcgtattccc attcgtataa agctttcgtt tcgtccaatc
     1021 cttcataaca ctgcaagcgg atcaactcgc gacctgtagc gcccgaccac gctttcgcca
     1081 attcggtttt gccgacgccc gccggacctt cggccaagat cggcttatcc aaacgctcgg
     1141 ccaagaacaa gactgtggca atttcgtcgg aaggaagata ttgcagttgg gtgagggcgg
     1201 ctttgacttc aggaatatct ttaaacatgc gtggctacct atgaaacgaa gatggactga
     1261 ttgtacccga tgatcaaaaa agcgccacac ttttggtcag catggcgctc ttttgagaga
     1321 ccctgtttgt tcatgtgaca actttgacct ttaccgcctg ctccaacgcg atccgtagat
     1381 cgacatcgct gggcggcgag gcaaacagta gacgcccatt gacatacacg ccgtcggaaa
     1441 taccccagcg ctcgacagtc gatcgatcat cggtgttgat gccgcgcaga gttatttgat
     1501 cgccatattc gtgcgacagc tcagtcaagc gatcgcgcac ctgcacccaa tgcgggcact
     1561 ggccgcacca gaaaatatca accgtcgttt ggctggactg cttcggttcg attggttgat
     1621 aacggcgcgg caaaaattcg ggcggactga tcttggccca agccttcacc atcaagcgcg
     1681 cgccatgatc ttcagccacc gtgataaagc catgcgtggc aaagagattg tatgacatgc
     1741 tcggccaagc cgcattggtg atcgtagcca agccgctgga cgatgcccga actttctctt
     1801 cgaccgcttc aatcagcgcc aagccggtgc cccgcccgcg tgcctgcggt tgaacataca
     1861 tgcagtcgat gaaggtcaga tcttgaccat gcaccggccg cggcgcgtgt tcgatcggca
     1921 gatattcaat atgtccgacc ggcgtcgtgc ccacataggc aatcagcaga tgcaacccct
     1981 tgaagaagcg gttgtaattc cagtagcgct tggcgttgac gccgctgccc agtgcttgca
     2041 aggctacttc acggttcgat gggcaatctt cggcaaagtt gtaattcata cacagcagcg
     2101 tctgctcttg cgtgtcttga aattcggaga tttgcataga ccctgctcct tacaccggct
     2161 cccacgacgg ttccggccgc acggcaaact cgccaatcgt cggctcaacc atgcgcatgt
     2221 aatctgacgt ggcgatcttc atcgtctcgc gatgcgagcc agcctggaag acgatgaacg
     2281 gctg
//
LOCUS       DASRTJ010000365         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_325103,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000365 DASRTJ010000000
VERSION     DASRTJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325103"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..501
                     /locus_tag="VFF70_07400"
     tRNA            427..501
                     /locus_tag="VFF70_07400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:459..461,aa:Met,seq:cat)
     gene            complement(754..2073)
                     /locus_tag="VFF70_07405"
     CDS             complement(754..2073)
                     /locus_tag="VFF70_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZY44558.1"
                     /translation="MTATIASLSTEQFDKLRDWSINQMMPLLDNEHRRLTAENRQWLA
                     TKLARAIEQVDARLPGDDWGRLVDETADAVIGYGIISPYLRDPSVTEIMVNGPHQIYI
                     ERAGKLIEVEAQYKSDDDMRRAIDNIIWPLGRKVDRSQPYVDARLPDGSRVNVIIPPC
                     AIDGTGLTIRKFPSKRLTVSDLIQSGSLTPNTADFLQACVAARLNIVVVGGTGSGKTI
                     LLNVISSFIPVGERICTIEDSADLRLSQRHVVRLETTPALPDGTGQVTIRDLVRNALR
                     MRPERIIVGEVRGGEALDMLQAMNTGHDGSLTTVHANSPRDAIARLETLVLMAGFDLP
                     QRVVRAQIASAVNLLVQQERLRDGTRKLTRITEVQGLEGENVVLQDIFMYQAVALNES
                     GKVEGSMKPTGIRPNFSPRLEQAGFKMSGEMFGAGAIGTILAPPRKR"
     gene            complement(2070..>2873)
                     /locus_tag="VFF70_07410"
     CDS             complement(2070..>2873)
                     /locus_tag="VFF70_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY44559.1"
                     /translation="QLQAVFERSEREPKPNSAVVIAVAGARSGVGATTLAVNLAAALT
                     ATASTILVDFEPRSKAAVQLGLVFFSGLQDKLERDPAGINRESVEAALTTHQRNGLRF
                     LAMDDRGIEPVRSATILQHLLAMCEVCVIDLGCEFGVARRALMQMADVCMLVVDSEPQ
                     ALIQAKRVIQSLSTVNLQPGALKLIGIHRLGTPVDIAQKMLRAALDHDLTAIIGPASE
                     ALLEALDRGQPIVLSDPDHPVAIQMRDLAFSLLETGPRVAEFAYGGKDS"
BASE COUNT          644 a          834 c          729 g          666 t
ORIGIN      
        1 tcataacccg gaggccagtg gttcgaatcc aatccccgca acacaaaaca ccgccaatca
       61 acaactggcg atgtttttat tttaggtacc accaaccgac tttaatacaa cctcaagtct
      121 cgataatcac gcgtgtaata aaccgagatc aataatcggg aaacaggatt aatcgcgccg
      181 ccgtccaccg gccatcgaga taatcctgtt tcaactgcgc gtgctggatc ttgccattgg
      241 gcgtgcgtgg aatggtatac agcttgacca gctacacgcg ccccggtctg aagacccatc
      301 gatctttcac ggtattgacg atatcgatca ccaacttttc gaacacttcg aatgttacac
      361 aacatttttt gatgtgacct tgcgatccag cttcttctcc ggtataatct cggtcgctca
      421 actctaggtg gcgtagctca gtggtagagc aggggactca taagcccttg gtcacaagtt
      481 caaatcttgt cgccaccacc aaactgccag cggctcgttc gattgaacaa gccgctgtgt
      541 tctcgcccgt agcctctccc agattttcgt gaggctgtaa actctctgtc cgtattgctc
      601 cccttaaaga tcgcggatat tgactgtgtt ggtggccagc tcaagatctt cagtaaaaag
      661 cgatcgagtc tcgataaaag ttgaggctcg atctaataaa atgaggcgat tccctgttgg
      721 ttgtatcgct tccgagacgt tctgttgagt gggctaccgt ttccgcggcg gcgccaatat
      781 cgtgccgatc gcgcccgctc caaacatctc gccactcatc ttaaagccgg cttgttccaa
      841 acgcggggag aagttcggcc gaatgccggt cggcttcatc gaaccttcca ctttgccgct
      901 ctcgttcaag gccaccgctt gatacatgaa aatatcttgc agcacaacgt tctcaccctc
      961 cagaccctgc acttcggtga tgcgcgtcag cttgcgtgta ccatcgcgca gccgctcttg
     1021 ctgaactaat aaattcactg cagatgcgat ttgtgcgcgc acgacgcgct gcggcagatc
     1081 gaaacccgcc atcaagacta aagtttctaa acgggcaatc gcatcgcgcg gactattggc
     1141 atgcaccgtc gtcaatgatc catcatgtcc ggtgttcatc gcttgcagca tatcgagcgc
     1201 ttcaccgcca cgcacttcgc ccacgatgat ccgttccgga cgcatgcgga gtgcatttcg
     1261 cacgagatcg cgaatcgtca cttgcccggt tccgtcgggc agcgccggtg tagtttccaa
     1321 tcgcacgaca tgccgctggc tgagccgcag gtccgccgaa tcttcgattg tgcagatgcg
     1381 ctcccctaca ggaatgaatg acgagatgac atttaatagt attgtcttgc cggaaccggt
     1441 gccgcccacc acgacaatgt tcaagcgcgc ggcgacgcag gcttgcagaa aatcggccgt
     1501 gttgggtgtc aagctgccag actggatcag atcgctgacg gttaatcgct tactgggaaa
     1561 ctttcgaatc gtcaggccgg tgccatcgat ggcgcacggt ggaataatca cattgacgcg
     1621 gctgccgtcc ggcagacgcg catccacgta cggctgcgat cgatctactt tgcggcctaa
     1681 tggccaaata atattatcga ttgcgcggcg catatcatca tcgcttttgt attgggcttc
     1741 aacctcgatc aacttgccgg cacgctcgat gtagatttga tgcgggccat tcaccataat
     1801 ctccgtcacg ctgggatcgc gcaagtatgg actgatgatg ccataaccga tcacggcatc
     1861 ggctgtttca tcgactaatc tgccccaatc atcacccggc aggcgggcgt ccacttgctc
     1921 gatcgcgcgg gctaacttag tcgccaacca ttggcgattt tcagctgtca accttcgatg
     1981 ttcgttgtca agcagtggca tcatttggtt aatcgaccag tcgcgcagtt tatcgaattg
     2041 ctcggtcgat aatgaagcga tcgtcgcagt catgagtctt tgcctccata ggcaaattcg
     2101 gcaacgcgcg ggccggtctc caacagcgag aaagcaaggt cgcgcatttg gatcgcaacg
     2161 ggatgatcgg ggtcgcttaa cacaatcggt tgacctcgat cgagtgcttc cagcaaagcc
     2221 tcggaggccg gtccaatgat cgctgttaga tcgtgatcca gtgccgcgcg cagcatcttt
     2281 tgggcgatgt ctaccggggt tccgagccga tggatgccga tcaactttaa ggcaccgggt
     2341 tgcagattga cggtgcttaa agactgtata acccgcttgg cttgtatcag cgcctgcggt
     2401 tcggaatcaa ccaccagcat acacacatcg gccatttgca tcagtgcgcg ccgggccacg
     2461 ccaaactcac aacccagatc gataacgcag acctcacaca tcgccaataa atgctgcaga
     2521 atggtggccg agcgcaccgg ctcgatcccg cggtcgtcca tcgccagaaa acgcagtccg
     2581 tttcgctgat gagtggtgag ggcggcttcg acgctctccc gattgattcc cgccggatca
     2641 cgttcaagtt tatcttgtaa tccactaaaa aaaacaagac ccaactgaac agcggctttg
     2701 ctgcgcggct caaaatcaac taagatggta gacgcagtgg cggtcaaagc ggcagccaga
     2761 ttgacggcaa gcgtggtggc tccgaccccg cttctcgcac cggccaccgc gatcacgaca
     2821 gcgctgttcg gttttggctc gcgctcagat cgttcgaaca ccgcttgcag ctg
//
LOCUS       DASRTJ010000366         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_325166,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000366 DASRTJ010000000
VERSION     DASRTJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325166"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFF70_07415"
     CDS             <1..437
                     /locus_tag="VFF70_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZY44560.1"
                     /translation="LNQASLLSTQAQNNRTFTVLLGAVAGISLLVGGIGVMNIMLVTV
                     TERTREIGLRKAVGAKRRDILLQFLIEAMTLSLIGGAVGILIGVGAAVGISSLSDQFQ
                     ANVSIVTVLLATGVSASVGLFAGLYPAVRAAQLNPIDALRYE"
     gene            complement(496..1764)
                     /gene="kynU"
                     /locus_tag="VFF70_07420"
     CDS             complement(496..1764)
                     /gene="kynU"
                     /locus_tag="VFF70_07420"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZY44561.1"
                     /translation="MAIRTDVEYALELDEHDELARFRDEFVIDDPDLIYLDGNSLGRL
                     PKRSIDRMKAVVEDEWGGRLIRGWNEGWFTAPQRIGAKIAQLIGADADEVIVSDSTSV
                     NFFKLVVAALRARSDRTKIVTDDLNFPSDMYILQGAIDLLDGDRKLQVIHAVDGLTVS
                     PETVDAAIDHTTALVTFTHTAFKSGFVYDMPLITKIAQRAGALMLWDLSHSIGALPID
                     LHAANADLAVGCTYKYLNGGPGAPAFLFVRRDLQEQLINPIWGWFGQKGQFDFGLEYR
                     SAPGINRFLTGTPNMLSLAAVEPAIDLMLEAGIDRLRAKSIKQTEYLIGLYDELLRPI
                     GFSLNSPRDYNRRGSHISIGHPEALRIDRGLIEDLKVIPDFRAPDNIRLGIAPIYTSF
                     AEIHEGVLRIKRVIEEKLYEKYPSERPEVT"
     gene            complement(1800..2714)
                     /locus_tag="VFF70_07425"
     CDS             complement(1800..2714)
                     /locus_tag="VFF70_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44562.1"
                     /translation="MFNRSAILLKFPWLAQRNRRMIIGNDLDALLSAQFLHDYLGWSI
                     AGFYNYSTIYFDPKTDLGECVWVDLDIYHADIASIGHHILKSSATDRIPKHRSSLNPN
                     LLRGIDQVNFKHKYPLGTIHLLLWLDDQSIKNRRPATLMLWLADSTWINAQIYRENVK
                     AWLNDWLPISELINTFDQTATGEFEEEMRDQVLSKIDIAALAEDDNIQRRSKHLDLGG
                     HQCVWNRPDELPKVRRVIDLIQRRFGWAAPCFPREFQAVEGVRHSAKLPDLIAKHGSL
                     DKFLDAEKVFSYVIPNKDRINYTTGIML"
     gene            complement(2765..3193)
                     /gene="arfB"
                     /locus_tag="VFF70_07430"
     CDS             complement(2765..3193)
                     /gene="arfB"
                     /locus_tag="VFF70_07430"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZY44563.1"
                     /translation="MPAKVIITPTMSIPLSEVQYRFVRSSGPGGQHVNKTSTQVELWF
                     DVLHSSALTEPQRQRLLHKLKNLIDSTGVLQLSEQSERSQFRNKQQVTEKFKSLLIAA
                     LRVPKPRRPTRPTKAAKEKRIASKKKQGQMKKARSKFYEE"
BASE COUNT          749 a          932 c          850 g          777 t
ORIGIN      
        1 tcctcaacca ggcctcactg ttgtcgaccc aggcacagaa caatcgcacc ttcacagtgt
       61 tgctcggcgc ggtcgcggga atctcgctgc tcgtcggcgg catcggcgtg atgaacatca
      121 tgctcgtcac cgtcaccgaa cgcacgcgcg agatcggtct gcgcaaagcc gtcggcgcga
      181 agcgtcgcga tattctgttg cagtttttga tcgaagcgat gacgttgagt ttaatcggcg
      241 gggcggttgg gattctaatc ggagtggggg ccgccgttgg catctccagt ttatcggatc
      301 aatttcaagc caacgtgtcg atcgtcaccg tgttattagc cacgggcgtt tcggcctcgg
      361 tcggtttgtt cgcaggattg tatcccgccg tgcgcgccgc gcaattgaat ccgatcgatg
      421 cgttgcggta cgagtaaacc ctcaccttcc ccgctccctt aaagggagag ggtgacgcga
      481 agcgtcgggt gagggtcacg ttacctcggg tcgttcactc ggatacttct catacaactt
      541 ctcctcaatc acgcgcttga tgcgcagcac accctcgtgg atttccgcga aagatgtgta
      601 gatcggcgcg atgcccaatc ggatattatc gggcgcgcga aaatccggaa tcaccttcaa
      661 gtcttcgatc aacccgcgat cgatccgcag cgcttcagga tgtccgatcg agatatgtga
      721 tccgcgccga ttgtaatcac gcggcgagtt taagctaaac ccgatcggcc tgagcagctc
      781 atcatatagg ccgatcagat attcagtttg tttgatcgat tttgcccgca ggcgatcgat
      841 gcccgcttcg agcatcagat cgatcgccgg ttcgaccgcc gcgagtgaca acatattcgg
      901 cgtgccggtg agaaaccgat tgattcctgg cgccgatcga tactccagcc cgaaatcaaa
      961 ttgtcccttc tgaccgaacc agccccagat cggattgatc aactgctctt gcaaatcgcg
     1021 ccgcacaaac aaaaatgcgg gcgcacccgg cccgccatta agatacttat aagtgcagcc
     1081 gacggcgaga tcggcattgg ctgcatgcaa atcaatcggc agtgcgccga tcgaatggct
     1141 caagtcccac agcatcaacg cccctgcccg ctgagcgatc ttggtgatca atggcatatc
     1201 gtacacgaat ccgctcttga acgccgtgtg ggtgaaagtc accaaagccg tggtgtgatc
     1261 gatcgctgcg tcgactgtct caggcgaaac ggtcaggcca tccactgcat ggatcacctg
     1321 aagtttccga tcgccgtcta acagatcgat tgcaccctgc aggatgtaca tgtccgacgg
     1381 aaaattcaga tcgtccgtca cgatcttcgt tcgatccgat cgcgcgcgca gcgccgccac
     1441 aaccaacttg aaaaagttca ccgaggttga atcgctcacg atcacttcat ccgcatccgc
     1501 gccgatcaac tgtgcgatct tcgcgccgat gcgctgcggc gcagtaaacc agccttcgtt
     1561 ccagccgcga atcaaccggc cgccccattc atcttcgacc actgccttca ttcgatcgat
     1621 gctgcgcttc ggcaggcgtc ccaatgaatt gccatcgagg taaatcagat ccggatcatc
     1681 gatcacgaac tcatcccgga aacgcgcaag ctcgtcgtgt tcgtcaagtt cgagggcgta
     1741 ttcaacatcg gtgcgaatgg ccatatgatt tctccggagc ggcgagcagc cgctcgccgc
     1801 tacaacatga ttccggtggt gtaattgatt cgatctttgt tcggaatcac gtacgaaaac
     1861 actttttcag catccaagaa tttatccagc gagccatgct tcgcaatcag atcgggcaac
     1921 ttcgctgaat ggcgaacacc ttcaaccgcc tggaactcac gcggaaaaca cggcgcagcc
     1981 cagccgaagc ggcgctgaat gagatcgatc acacgcctga ctttcggcaa ttcatccggc
     2041 cgattccaca cgcattgatg tccgcctaaa tccagatgct ttgatcgacg ctggatgtta
     2101 tcgtcctcgg ccagtgcggc aatatcgatc ttgctcaaga cttgatcacg catttcttcc
     2161 tcgaactcgc ccgtcgcagt ctgatcaaac gtgttgatca attcactaat cggcagccaa
     2221 tcattcagcc acgctttcac attctcccga taaatctgcg cgttgatcca ggtcgaatcg
     2281 gccagccaca acatcaacgt cgccgggcgg cgattcttga tcgattgatc gtccagccac
     2341 aacaacaaat ggatcgtgcc taacggatat ttgtgcttga agtttacttg atcaatcccg
     2401 cgcagcaagt tgggattgag gctcgatcgg tgcttgggga ttcgatcggt tgcagaggat
     2461 ttcaaaatgt gatggccgat cgaagcgatg tctgcgtggt aaatatccag atcgacccag
     2521 acacattcac ccaaatcggt ttttggatca aagtagatgg tggagtaatt atagaagcct
     2581 gcgatcgacc agcccagata atcgtgcagg aactgcgcag agagcagcgc gtccagatcg
     2641 ttgccgataa tcatgcggcg gttgcgctga gccagccacg ggaatttgag caggatggca
     2701 gatcggttaa acacagaatg cgttctcaat gacttttgaa gaaagtaagt gacgcctcac
     2761 gtcgtcattc ttcataaaac ttgctgcggg cttttttcat ttgcccctgc tttttcttac
     2821 tcgcaatccg tttttctttg gcggctttcg tcgggcgtgt aggtcgtcgg ggtttgggca
     2881 cccgcaaagc agcgatcaat agagacttga atttttcagt gacctgctgt ttattccgaa
     2941 actggctgcg ttccgattgt tcgctcagct gcaacacgcc cgtgctatca ataaggttct
     3001 tgagtttgtg caagagccgc tggcgctgtg gctcagtcag cgccgaagaa tgcaggacgt
     3061 cgaaccacag ctcgacctgg gtcgacgttt tgttgacgtg ctggccgccc ggcccgctgc
     3121 tgcgcacgaa gcgatactgc acttcggaca gtggaatact cattgttggc gtaataataa
     3181 ctttggctgg catttctatt cgctttcagc accgacaaat atcatagcac gaacaggcgt
     3241 cgtgacacta tcatttatcg ataacgctgt ataagataac ttcacaagga gattcaagca
     3301 tgacacca
//
LOCUS       DASRTJ010000367         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_161182,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000367 DASRTJ010000000
VERSION     DASRTJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161182"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFF70_07435"
     CDS             <1..247
                     /locus_tag="VFF70_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44564.1"
                     /translation="ALKHVMAGRTSIVIAHRLSTILAADLILVMDRGQIVERGTHAEL
                     LGQGGVYAHLYETQFKGTLAPESMLLMDNLPAQVESP"
     gene            complement(557..1036)
                     /locus_tag="VFF70_07440"
     CDS             complement(557..1036)
                     /locus_tag="VFF70_07440"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HZY44565.1"
                     /translation="MKIVFGIELNAQHLRIGLIAGLIAVLITTAVNVASRVVGLFPEA
                     MDLKNMAEFFVNPARDPATAFVLGTILHILLGGVAGVVYGLLIKSFSPIGGVLFMFAN
                     WLLMMLALFPVTNRGFFGLNQGSMMPVATFILNMLYGVVFGVLARRMDAAASQPKIP"
     gene            complement(1033..1779)
                     /locus_tag="VFF70_07445"
     CDS             complement(1033..1779)
                     /locus_tag="VFF70_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY44566.1"
                     /translation="MIRLLRFGTPTQSPPLFAIPGLDGSIGSIQPVVEALAKQREVIV
                     ADFSTETSSTLEALATEIAKVIKTEGRDTIDVMGQSIGTILAAQVASLHGLPVRKIVL
                     TCTFTICRWNLLRIVVALSKLAPAWLYRLTSWLSLAISCGPVGNGGHHPAFAAARDAN
                     KNAIAKRTAWQINRDFSHDLVRIRVPLLILMGGNDRFVPDAKKEITKLRRMFEPQPAA
                     SIDVIPKAGHIFLPSAAVALAVAEIEEFLR"
BASE COUNT          490 a          552 c          564 g          451 t
ORIGIN      
        1 agcgttgaag cacgtgatgg ccgggcggac cagtattgtt attgcgcatc gcttaagcac
       61 aattctcgcc gccgatctga tcttggtgat ggatcgcggt cagattgttg agcgtggtac
      121 ccatgctgaa ttgttagggc agggcggagt ctatgcacac ttatatgaga cacaattcaa
      181 aggaacactt gcgcctgaga gcatgttgct gatggataat ttacccgcgc aagttgaaag
      241 tccgtgatat ggtaatgcgg tgagcaagcg aacgcgacgg cattcagtgg ttgggaggta
      301 atcatcccgc aactacttac cgaacggggc aaggtccctg tgacccgttt gtccgatggg
      361 cttagcactg ttgagaatca tgaaatacga ttgccggcga gcacagggtt tggaatcaat
      421 ttgccgagga ttaacaactc ggttttagta ttggtcgttg agccagtcca cttcgttctt
      481 cccgtctgag tcctgatata gcaatcctct caatgctacc cttcgagagc gcggggcgcg
      541 accaataaat gccacgttac gggatctttg gttgcgacgc tgctgcgtcc atgcgccgcg
      601 ccagaacacc gaacacgacg ccatagagca tgttcaagat aaacgttgcg acgggcatca
      661 tcgatccttg attcaagccg aagaaacccc ggttcgtcac agggaagagc gccaacatca
      721 tcagcagcca gttggcgaac atgaagagca cgccgccgat tggactgaac gacttaatca
      781 gcagcccata caccacgccg gcaacgccac ccaatagaat gtgcaggatc gtgccgagca
      841 cgaaggccgt cgccggatcg cgcgctggat ttacaaaaaa ttcagccatg tttttcagat
      901 ccatggcttc aggaaacaag ccgactactc gacttgcgac gtttacagcg gtcgtgatca
      961 gaactgcgat caggccggcg atcaagccaa tgcgcaagtg ctgtgcattc aattcgatcc
     1021 cgaacacgat cttcatcgca agaactcctc gatttcagcg acagccaacg cgaccgcagc
     1081 cgatggcagg aagatatggc cggccttggg tataacgtcg atgctcgctg cgggttgcgg
     1141 ctcgaacatc ctgcgcaact tggtgatctc tttcttcgcg tcgggaacaa atcgatcgtt
     1201 gccacccatc aaaatcaata acggcactcg aatgcgaacc agatcgtgag agaaatcgcg
     1261 attgatttgc cacgcggtcc gttttgcgat ggcattctta ttcgcgtctc gcgcagcagc
     1321 gaaagcggga tgatgtcctc cgttgccgac cggcccgcat gaaatagcga gactcagcca
     1381 cgaggtgagg cgatacaacc acgcaggcgc cagctttgac aatgcgacaa ctattcgaag
     1441 caggttccat cggcaaatcg tgaaggtaca ggtcagcacg attttgcgca caggcagacc
     1501 atgcaaggat gcgacttgcg cggccaatat cgtgccgatc gattgaccca tcacgtcgat
     1561 ggtgtctcgg ccttccgttt tgatcacctt cgcaatttcc gtcgccagcg cttccagtgt
     1621 cgaactcgtt tctgtgctga aatcggcaac gatcacttcg cgctgcttcg ctaacgcttc
     1681 gacgacgggt tgaatactgc cgatcgatcc atcaagaccg gggatcgcaa acagcggtgg
     1741 cgattgggtt ggcgtgccaa aacgtaagag tcggatcata atgattgttg gaggccatgc
     1801 cgggcggagc tagagatctc tatcgcggcc aacctatgag catgtcgtgg tggagctggg
     1861 agctgtgcac gtggtcctcg ctcaatgcgt tcgctccgaa taacacgcca ggagtctcaa
     1921 tatcattaac ttaccttccg cgaatcgcgc aggttgtcaa tcccgcgcac gcgagaattc
     1981 aatccgcaat gactggacac gcgtctgcgc gagtgtgatg gtgcgtaaca tgagtcatcc
     2041 atgatggcgt gcgaacc
//
LOCUS       DASRTJ010000368         3852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_249890,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000368 DASRTJ010000000
VERSION     DASRTJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249890"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..374
                     /locus_tag="VFF70_07450"
     CDS             108..374
                     /locus_tag="VFF70_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44567.1"
                     /translation="MPKLTAAEMNLLQALANGYTLKSHRYLDGSKEYQLRALDGSIAE
                     VSRARVETLVENHLIDDNKKFPAATYWLTETGISKLNERPSTRS"
     gene            499..1380
                     /locus_tag="VFF70_07455"
     CDS             499..1380
                     /locus_tag="VFF70_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44568.1"
                     /translation="MKTRNLLLIALLTTSMFLTACQQKPSNVPADPVAAVKIIADKQK
                     EVTTQHVDLTLTLSIQADGIKTDPSNPSVASAAAFLKNFKANVTVGGDVDTAKSNFSL
                     SGSADIGALTALLAQGADKLTFDLIKVDDTMYSRTGTDKWTSSPVAKTTSTTASVTAT
                     QATAQIAEVIKNAAKAEKLGDENIGGTNTYHYKVTLDALALIDQALAVAAADPKATPP
                     DPKQVQQAKDLLKDSVLELDMWVGQEDLYYRQVTLHIKLDLKNIPENPGATALVDLLL
                     KATFSNVNKPVNIVPPQ"
     gene            1568..3661
                     /gene="fusA"
                     /locus_tag="VFF70_07460"
     CDS             1568..3661
                     /gene="fusA"
                     /locus_tag="VFF70_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZY44569.1"
                     /translation="MKDYPTAALRNIALIGHGSAGKTSLSEAMLFSSGALSRQGRVED
                     GSTVSDFDEEEVRRHISLTSSLLPLEWHDRKLNILDTPGYPDFAGEVKGAMRVADAAL
                     IVIDSVAGVEVGTELAWSYCGERNLPRLILINKMDRENANFSRAFESLHTAFGVNFVP
                     LQLPIGSQAAFEGVIDLITLKARRGPKGEAGDIPANMTDEVNEARLKLMEAAAEGDDD
                     LIMKYLDGQELTEEELRRGLRVAIRKNTLVPVLCSAGTANIGVSTLLDALAEFVPAPS
                     DLDPEPAKNVATNTDELIKPDGSGTLGALVFKTTADPFVGKLTYFRVYGGTLATDSRV
                     FNPNKNQEERLGQLYVMRGKEQIPVHSIKAGDIGAVAKLSATGTGDTLCDKAHPITFA
                     PPTYPRALASVAIEPKSEADSAKMGPSLTRLVEEDPTLHWFNDPSVKQTILEGLGDSH
                     LDVAVRRSKAKFGVELITIPHKIPYRETITKPQQAMHRHKKQSGGAGQFGEVHMRVEP
                     NKSKGYEFLWEVVGGAVSSSYKSSIEKGIKSVMESGAIAGYPVVDVKVAITDGKEHAV
                     DSKPIAFETAGREAFKKALHGAGPVLLEPIMKVSITVPEANMGDVLAGINTKRARVQG
                     MDQVSGKSVIIALVPLAEVQHYAADLRSITQGRGVYSMEFDHYDEVPHQIAQGIMEAA
                     QKEHPHLRIAESDHA"
BASE COUNT          999 a          989 c         1054 g          810 t
ORIGIN      
        1 ctacaagagc atgtgtccga tctcggttga atgcgaaaat aagatcggag cagtttttga
       61 gtgacgggta cgcgatcagc tcgtttttgc ttatcgcgaa gattcacatg cctaaattga
      121 ctgccgctga aatgaattta ctgcaggctt tggcgaatgg ctacactttg aaatctcacc
      181 gctacctcga cggatcgaaa gagtaccagc tgcgtgcgct cgatggatcg atcgcagaag
      241 tatcgcgcgc gcgcgtcgag acgttggtcg agaatcatct gatcgatgac aataaaaaat
      301 ttccggctgc aacttattgg ctgactgaaa caggtatctc caagttaaat gaacgaccga
      361 gtacgcgttc ataaatacgg ctgacgagta cttaatccta gttgacctcg ctaaacgctt
      421 attcgcggta taatacaggt aactccaaca gcgcagactg cgcaaactat gttgaataaa
      481 tgaaggaagg aataagagat gaagactcgt aatttgttat tgatcgcact gctgacaacg
      541 tcaatgttct tgacggcctg ccagcaaaaa cccagcaatg tgccggccga tccggtcgcg
      601 gcggtgaaaa ttatcgccga caagcaaaaa gaggtcacga cccagcacgt cgatctgaca
      661 ctgaccttgt cgattcaggc ggacggaata aagaccgatc ccagtaatcc aagcgtcgct
      721 tcagccgcag cgtttttgaa gaacttcaaa gccaacgtga ccgtcggcgg cgacgtggat
      781 acagctaaga gcaactttag cttaagcggt tcggcggata ttggtgcgtt gactgctttg
      841 ttagcccaag gcgctgataa gctgaccttc gatttgatta aggttgatga caccatgtac
      901 tcgcgcacgg gcacggataa atggacctca tcgccggtcg cgaagacaac cagcactacc
      961 gcttcggtga ctgcaactca agcgacagct caaatcgccg aggtcatcaa gaatgcggcc
     1021 aaagccgaaa agttgggtga tgaaaacatc ggcggtacca acacctatca ctacaaagtt
     1081 actttggatg ctctggcgct gatcgatcaa gcgttggctg tggctgccgc cgatcccaaa
     1141 gcaacaccgc ccgatcccaa gcaagttcaa caggccaaag acttgttgaa agattccgtg
     1201 ctcgaattgg acatgtgggt gggccaggaa gatttgtatt accgccaagt gacattgcac
     1261 atcaagctgg atctcaagaa cattccggaa aacccggggg caactgcgct ggtcgatttg
     1321 ctattgaaag ccaccttcag caacgtcaac aagccagtca acatcgtccc accgcagtaa
     1381 accgatttcg cacggaagat cgatcgagta agcaactgac ctcccgaagg tttgaaacct
     1441 tcgggaggtt tttgatcgat caacatgtca atcgacaggc agaatcggac gcttcgtcac
     1501 attgacaata cgcgctggct tgaattacaa tactctagca gtctatctcc aatacccggg
     1561 aggtttgatg aaggattacc ccactgccgc cttgcgcaac atcgccctga tcggccatgg
     1621 ttcggccggg aagacctctt taagcgaagc gatgttgttt agcagcggcg cactcagccg
     1681 gcagggacgc gttgaagatg gcagcaccgt gtcggatttt gatgaagaag aagttcgccg
     1741 gcacatttcc ctcaccagtt cgttgttacc ccttgaatgg cacgatcgta aactcaacat
     1801 ccttgacacg ccaggctatc cggatttcgc cggcgaggtc aagggcgcaa tgcgcgtggc
     1861 cgatgccgca ttgatcgtga tcgatagcgt agccggtgtc gaggtaggca ccgagttagc
     1921 ctggtcgtat tgcggcgagc gtaatctgcc gcgcttgatc ctaattaata aaatggatcg
     1981 cgagaacgcg aatttttcgc gcgccttcga atcgctgcat accgcattcg gtgtgaactt
     2041 tgtgccgctg caacttccga tcggcagtca ggcggcgttt gaaggcgtga tcgatttgat
     2101 tacgctcaag gcgcgccgcg gccccaaggg cgaggccggc gacatcccgg caaatatgac
     2161 ggacgaagta aacgaagcgc ggttgaaatt aatggaagcc gcggctgaag gcgatgacga
     2221 cttgattatg aaatacctcg atggacagga attgaccgaa gaggaattgc ggcgcggtct
     2281 gcgggtcgct attcgcaaaa atactttagt gccagtgctg tgcagcgcgg gcaccgcgaa
     2341 catcggtgtt tccacgctat tggatgcgtt ggccgaattt gtgcccgcgc cgagcgatct
     2401 ggatcccgaa ccggcgaaga acgttgccac gaataccgat gaattgatca agcccgatgg
     2461 atcgggcacg ctcggcgcat tagtgttcaa gacaacggcc gatccctttg tcggcaagct
     2521 cacatacttt cgcgtttacg gcggcacact cgcgacggat tcgcgcgtct tcaacccgaa
     2581 caagaatcaa gaagaacgac tagggcagtt gtacgtcatg cgcggcaaag aacaaattcc
     2641 ggtgcattcg atcaaagccg gcgacatcgg cgcggtggca aagttgtccg ccaccggcac
     2701 gggcgatacg ttatgcgata aagctcaccc gatcactttt gcgccgccca cctatccgcg
     2761 tgccttagcc tccgtggcga tcgaaccgaa gtcggaagcc gattcagcca agatgggccc
     2821 ttcactcacc cgattggtcg aagaagatcc aacgctgcat tggttcaatg atccgagtgt
     2881 caagcaaacg atcctggaag gactcggcga cagtcatctc gatgttgccg tccgccgatc
     2941 gaaggccaag ttcggcgttg aactcatcac catcccacac aagattccgt atcgcgaaac
     3001 gatcaccaaa ccgcagcaag ccatgcaccg ccacaagaaa caatcaggcg gcgctggaca
     3061 atttggcgaa gtacacatgc gtgttgagcc gaacaaaagt aaaggctatg aatttctgtg
     3121 ggaagttgtc ggcggcgcgg tatcgtcttc ctataagagc agcatcgaaa aaggaattaa
     3181 gagcgtaatg gaatccggcg cgattgcagg ctacccggtg gtcgatgtga aagttgccat
     3241 caccgacggc aaggagcacg cagtagattc caagccgatc gcgtttgaga cggctggacg
     3301 agaagccttc aagaaagcgc tacatggcgc aggtccggtg ctgcttgagc cgatcatgaa
     3361 agtatcgatc actgtgcccg aagcgaacat gggcgatgtg ttggccggta tcaacaccaa
     3421 acgcgcgcgc gtacagggca tggatcaagt cagcggcaaa agtgtcatca tcgccctggt
     3481 accgctggcc gaagttcagc actacgccgc cgacttgcga tcgatcacgc agggtcgcgg
     3541 tgtctacagc atggaattcg atcactacga tgaagtgccg caccagatcg cgcaagggat
     3601 catggaagcc gcgcagaagg aacacccgca tttgaggata gcggaatcgg atcacgcgta
     3661 aaaaagttcg ggcagcagtt gaactgcaac cagaacgtca actaaaaaca aaagcccgat
     3721 cgtgtgaatg cgatcgggct tttgtttcga cttgagagtt tgggtcattc ggcagagtgc
     3781 gatgtttatg gtttaggagt atttgtgagg gaaataggct ctcgtaaatt gtaagtcgga
     3841 ccagtagatg tc
//
LOCUS       DASRTJ010000369         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_250073,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000369 DASRTJ010000000
VERSION     DASRTJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250073"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..2021
                     /locus_tag="VFF70_07465"
     CDS             300..2021
                     /locus_tag="VFF70_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY44570.1"
                     /translation="MRRRFELFFNFNRPAFGLGDGLIILIIAVLIYLGTRLAINAPAS
                     VSGPTIDLSPRALPWYAFLSTGRMALAYLLSLIFTLVYGYAAARNRTAEKVLMPLLDV
                     LQSIPILSFLPVVLLSLTSILPQRVATELAAVILIVTSQAWNMTYSFYQSVKTIPTEL
                     REASAIFRFSGWLRFKTMELPFAALGLIWNSMMSWAGGWFFLMASEIFTVGSKDFRLP
                     GLGSYLQTAANEGDVRAIFFGLGTLILVIVLLDQLIWRPILVWANKFKVEMVESDTPP
                     ESWFYDLLSRSWIIKQFANRVWHPLNEKIDRFMGRIMQPDRSSAPNRTSRRSILGPLL
                     AIAALIGLIFGGISAIGLLIQLPIADYGRIGISLIATFLRVAFSLVIAVAWTIPVGVL
                     IGSNQRLSAYLQPIVQIVASIPATALFPVIVLALINLTGGLDVSAVILMLLGTQWYVL
                     FNVIAGTSAIPQDLSYTTSLLQVKGWARWRTLILPALFPYLITGMITASGGAWNASII
                     AEHIEFSGRSISTTGVGALIAEATATGNYPLLLASTLALILTVVTINRTLWRRLYKVA
                     EEKYKME"
     gene            2074..>2690
                     /locus_tag="VFF70_07470"
     CDS             2074..>2690
                     /locus_tag="VFF70_07470"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44571.1"
                     /translation="MITNTNTNGALLEVKNVSRYYGKGAKRFLAVHDINLKINPGEFV
                     CLLGPSGCGKSTLLRMITGLNPTSEGQVLYRGEPQSGVNPHATIVFQSFALYPWLSVQ
                     QNVEVALKARGVDEVTRKAKAIQLLDKVGLDGFESAYPRELSGGMRQKCGFARAMAVE
                     PELLCLDEPFSALDVLSAEALRGELMELWTSKSIPTQAILMVTHN"
BASE COUNT          556 a          789 c          705 g          640 t
ORIGIN      
        1 tccatcctcc gtcctcggtc gatcagttta gcttcaacct aaaaatcgat cgacatccct
       61 ctcagcaaac tctcatcaaa atttttcaat ttgggtattg acaaacgatc atgcgcgggt
      121 gtacactcga cacgttggaa aaaatcatgg aagaacaaac tgactcactc gatcccgatc
      181 agaaacagtt gcaccaccca ctccgcctca tggccgcgtg atagttcaac acgcctagcg
      241 catgctgatt agtccccgcg cccatgtggc cggggatttt tgttttcaga aagacttcta
      301 tgcgccgccg tttcgaactc ttcttcaact tcaatcgacc cgccttcggc ctcggcgacg
      361 ggctgatcat tctgatcatc gccgtgctga tttatctcgg cacacgcttg gcaatcaatg
      421 cgcccgcctc ggtcagcggc cccaccatcg atctatcgcc gcgcgcgctg ccgtggtatg
      481 cgtttctctc gacgggccgg atggcgcttg cgtatctgct ctcgttgatc ttcacgctcg
      541 tctacggcta cgccgccgcg cgcaatcgca ccgccgaaaa agttttgatg cccctgctcg
      601 atgtcctgca aagcattccg attctgtcgt tcctgcccgt cgtcctgctg agcctcacat
      661 cgatcctgcc gcagcgcgtc gcgaccgaac tcgccgccgt gatcttgatc gtcaccagtc
      721 aagcctggaa tatgacttat agtttttatc aatcggtcaa aacgatcccg accgaactgc
      781 gcgaagcctc agcaattttt cgctttagcg gctggctgcg cttcaaaacg atggaactgc
      841 ctttcgcggc gctcggcctc atctggaact cgatgatgtc gtgggcgggc ggctggttct
      901 tcctcatggc ttcggaaatt ttcacggtcg gctcaaaaga ctttcgcttg cccggcctcg
      961 gcagttattt acagaccgcc gcgaatgaag gtgacgtgcg cgccatcttc ttcggcctcg
     1021 gcacgttgat cctggtcatt gtcctgctcg atcaattgat ctggcggccc atcctcgtct
     1081 gggccaacaa attcaaagtc gaaatggtcg aaagcgacac gccgcccgaa tcgtggttct
     1141 acgatctttt aagtcgatca tggatcatca agcagtttgc caatcgcgtc tggcatccac
     1201 tcaatgagaa gatcgatcga ttcatggggc gcatcatgca gcccgatcga tcctctgcgc
     1261 ccaatcggac gagccgccga tcgatcctcg gccccttgct cgccatcgct gcgctgatcg
     1321 gcttgatctt cggcggcatc agcgcgatcg ggttgctgat ccaacttcca atcgcggatt
     1381 acggacgaat cggtatcagc ttgatcgcca cgttcttgcg cgtggcgttc tcgttggtga
     1441 tcgcggtcgc ttggacgatc ccggtcggcg tgttgatcgg atcgaatcaa aggttgtcag
     1501 cgtacttgca acccatcgtc cagattgtgg cctcgatccc ggcgaccgct ctcttccctg
     1561 tcatcgtctt agcgttgatc aacctcaccg gcggcctcga tgtttcagcg gtcatcttga
     1621 tgctgctcgg cacacaatgg tatgtgttgt tcaacgtcat cgcgggcacg agcgcgattc
     1681 cgcaggactt gagttacaca acgtcattgt tacaagtcaa aggctgggcg cgttggcgca
     1741 ccttgatctt gcccgcgttg tttccatatc tgatcaccgg catgatcacg gccagcggcg
     1801 gcgcgtggaa cgccagcatc atcgccgagc acattgaatt cagcggacga tcgatcagca
     1861 cgacgggcgt cggcgcgttg atcgccgaag cgactgcgac gggcaactat ccattgctgc
     1921 tggcctccac gctcgccttg attctcactg tcgtgaccat caatcgaacg ttgtggcgca
     1981 ggttgtataa agttgcggaa gaaaaatata aaatggaata aaacgttata acgttccaac
     2041 gttttaacgc atcccccatc atcggaggtt actatgatca ccaacaccaa caccaacggc
     2101 gccctgctcg aagtcaaaaa cgtctcacgc tattacggca aaggcgcgaa gcgatttctg
     2161 gcggtgcatg acatcaatct caaaatcaat ccgggcgaat ttgtgtgctt gctcggccca
     2221 tcgggttgtg gcaagtctac tctgctacga atgatcaccg gccttaatcc gacgagcgaa
     2281 ggccaagttc tgtatcgcgg cgaaccgcaa tcgggcgtga atccacacgc cacgatcgtg
     2341 tttcaatctt tcgcgctgta tccgtggctg agcgttcagc aaaatgtcga agtcgctttg
     2401 aaagcgcgcg gcgttgatga agtgacgcgc aaagcgaaag cgattcagtt gttggacaaa
     2461 gtcggcctcg atggattcga atcggcctat ccgcgcgaac tatcgggcgg catgcggcag
     2521 aagtgcggct tcgcacgcgc gatggcggtg gagccggagt tgttgtgcct cgatgaaccg
     2581 ttcagcgcgt tggatgtgtt gagcgccgag gcgctgcgcg gcgaattgat ggaactgtgg
     2641 acaagtaaat cgatcccaac gcaggcgatc ttgatggtga cgcataacat
//
LOCUS       DASRTJ010000370         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_123266,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000370 DASRTJ010000000
VERSION     DASRTJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123266"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VFF70_07475"
     CDS             <1..103
                     /locus_tag="VFF70_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZY44572.1"
                     /translation="VGDGFRNLDEGQQVEFTVQQGPKGLQAADVRAL"
     gene            401..622
                     /locus_tag="VFF70_07480"
     CDS             401..622
                     /locus_tag="VFF70_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44573.1"
                     /translation="MDQHRVFYKCETCLREALTPEDCHSHQMVEVDAGVEGDEVTLPV
                     TDEAGHLLSHAPKWWVYRRQAITKGLPHR"
     gene            794..1708
                     /gene="trxB"
                     /locus_tag="VFF70_07485"
     CDS             794..1708
                     /gene="trxB"
                     /locus_tag="VFF70_07485"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZY44574.1"
                     /translation="MENVIIIGSGCAGLTAAIYAARASLDPLVITGSELGGQIATTTD
                     VENYPGFPTGLTGPELSEQMKQQAERFGTRFEFDEVVEVDMKSKPIVVKTHGTTYEAK
                     SLIIASGASPRKLDVPGEKELTGRGVSYCGTCDGFFFKNKEIMVVGGGDSALQEGLFL
                     TKFGSRVSIVHRRDALRAGPALIDRAHKNEKMNFVWNKVVTSINGNGAVKSVMLKDTQ
                     TGAETETPVDGLFIYIGHLPNTWLYKGQLDMDAEDYLIVDPYLHTNLTGVFAAGEVHD
                     KHFRQAIVSAGFGAMAAMEAEKYLASLE"
     gene            1749..>2070
                     /locus_tag="VFF70_07490"
     CDS             1749..>2070
                     /locus_tag="VFF70_07490"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY44575.1"
                     /translation="MPYLDLNNERIYYALYRGELIPSRSIILIHGAGENHLVWPAGLR
                     RLPDSTVYAIDLPAHGKSTGNGRDSINGYVAWLKIFLDALKIQRAFIVGHSMGSAIAQ
                     MFALT"
BASE COUNT          493 a          549 c          581 g          447 t
ORIGIN      
        1 cgtcggcgac ggtttccgca acctcgacga aggtcagcag gtggagttca ccgtccagca
       61 aggccccaag ggcttgcagg ccgcagacgt ccgagcactg taaaccaaca ggtacgacgt
      121 ccaaaccata agcctcgatc aaatttgatc ggggcttatt tttttgtcga tggttgggac
      181 ggcgcatgcg cacactcatt tagatcgatc ttgatcctcg ccacaatcga taagccgatt
      241 cattgtgatc cattgacgag tgcgtcgccc ctacgtttat ggtctgtgca gccccgattg
      301 ttttagcgac gcgaatcaga ataatctttc gcgccgattg gttgctcgat catccacttg
      361 gcagtacaat cattgaagag atatagaagg gaggcgcgac atggatcagc atcgagtctt
      421 ttacaaatgc gagacgtgtt tgcgcgaagc attaacgcca gaagactgtc acagccacca
      481 gatggtcgaa gtggatgccg gtgtcgaagg cgatgaagtg actctgccgg tgacggacga
      541 agccggtcat ttgttatcgc acgcaccgaa atggtgggtg tatcggcgcc aagctattac
      601 aaaaggattg ccgcaccgct aagaagaatt gaatcgtctt ggcgaggact ctgaatgaag
      661 tgaagatgca agaggacgaa gcgatctccc tgactggtgg cagctattgc tatcaatgac
      721 tgggattgct tcgtcgcaaa aaacgctcct cgcaatgacg gatgagaagc actttttaat
      781 ggagaagtaa atcatggaga atgtcatcat tatcggttca ggctgcgcgg gtttgaccgc
      841 agcgatctat gccgcgcgcg ccagtcttga tccgttggtc atcaccggca gcgaactcgg
      901 cggacagatc gccaccacga ccgatgttga aaactatccc ggttttccga ccggattgac
      961 cggccccgaa ctgtctgagc agatgaagca gcaagccgaa cgcttcggca cgcgcttcga
     1021 atttgatgaa gtggttgaag tcgatatgaa aagcaaaccg atcgtggtca agacgcatgg
     1081 cactacctat gaggccaagt cgttgatcat cgccagcggc gcgagtccac gcaaactcga
     1141 tgtgccgggc gaaaaagaat tgaccgggcg cggcgtcagt tactgcggca cgtgcgacgg
     1201 cttcttcttc aagaacaaag agatcatggt tgtcggcggc ggcgacagcg ccctgcaaga
     1261 aggactgttc ctgactaaat ttggatcgcg cgtctcgatc gtgcatcgac gcgacgcgct
     1321 gcgcgccggg ccggccttga tcgatcgagc gcacaagaat gaaaagatga atttcgtttg
     1381 gaacaaagtt gttacctcga tcaacggcaa cggcgcagtc aaatccgtga tgctgaaaga
     1441 tacccaaact ggcgccgaga cggaaacgcc ggtcgatggg ctgttcatct acatcggcca
     1501 tctgccaaac acctggctgt ataaaggcca gctcgatatg gacgctgaag attatctgat
     1561 cgtcgatcct tatttgcaca ccaacctgac gggggtcttc gccgcgggcg aagtgcacga
     1621 caagcatttc cgccaggcga tcgtctccgc cggtttcggc gcgatggcgg ctatggaagc
     1681 ggaaaaatat ctcgccagcc tcgaatgaca tcgtagaggc gagcggctgc tcgccccgtt
     1741 cttttcaaat gccctacctc gatctgaaca acgaacgcat ctactacgcc ctgtatcgcg
     1801 gcgaactgat tccatcccga tcgatcatct tgattcacgg cgcgggcgaa aaccatttag
     1861 tctggcccgc cggattgcgg cgcttgccgg actcgaccgt gtacgcgatc gatctgccgg
     1921 cgcatggcaa gtcaacgggc aacggtcgag attcaattaa tggctatgtt gcttggctga
     1981 aaattttcct cgatgcgctg aagattcaac gggcgttcat cgtcggccat tcgatgggca
     2041 gcgccatcgc gcagatgttt gcgctcacgc
//
LOCUS       DASRTJ010000371         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_98354,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000371 DASRTJ010000000
VERSION     DASRTJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98354"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VFF70_07495"
     CDS             complement(<1..1388)
                     /locus_tag="VFF70_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HZY44576.1"
                     /translation="MRQVTFRDRLRYRFDNIMSRGSIALLIGLAIVSLIIIAVAGAII
                     AAGGITQDDGTQVGFFEAAWLSLVRTLDSGTFGGDSGIAWRVVMLGVTLGGVFVVSAL
                     IGIINNGLENRMDELRKGRSFVIEQKHTLILNWSPKIFTIISELVAANANQAKPRIVI
                     LAEKDKVEMEDEIATQIESLGKTKVVCRTGSPLDISDLEIVNPHAARSIIILAPTDSS
                     DPDSEIIKTILALTNNPNRRNDPYHIVAEIGDPKNLAVARMVGKDEVEMIVTGDLISR
                     IIVQTSRQSGLSIVYTELLDFGGDEIYFKEEPALVDKTFGDALMAYEDSTIIGLRPRD
                     GSPLLNPPMRTQIQSGDQLIAISEDDDTIKLSSLIGSWDNLDRRLSIDRSAIHNAPRS
                     IAKPERTLILGWNGRGPAIVAGLDQYVAPQSEITIVADMAVSAGDIERMQSAVRNQKI
                     VFQQDNTTDRAML"
     gene            1626..2996
                     /locus_tag="VFF70_07500"
     CDS             1626..2996
                     /locus_tag="VFF70_07500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY44577.1"
                     /translation="MSSSTADVVICGAGICGIAAAYHLTVTQKIKNVVLIDERPPLTL
                     TSDKSTEAYRNWWPGPGDAMVQLMNRSIDWLERWADATHNRFVLNRRGYAYFTADPNR
                     IPDFKLAAAEAAMLGAGPVRLHVDRFSDRSYLPIDPHRFADQPIGCDLVLDRSLILDR
                     FPCVNENVVALVHPRRCGWFSGQGLGMYLLEQAKAHGARLIEGKIDRVDVKSNRVHSI
                     HYGLELIDTNNFVIAAGPYLKEVGQLIGVDIPVFNELHVKIAFKDHLGIIPRDAPLMI
                     WTDPQFLPWTAEEREALQEDEDTRWLLNEFPGGVHLRPEGGGESQIVLMLWPYHVSPV
                     EPKWPPELDPFFPDVVLRGLSTMIPDMKKYFDKTPKSSYDGGYYTKTPENRPLASKLP
                     IDGAFACGAVSGYGLMASAAIGELLAAQVAHTELPSYAPAFSLDRYKDRAYQKLLENW
                     GSSGQL"
BASE COUNT          746 a          865 c          879 g          793 t
ORIGIN      
        1 agcatcgcgc gatcagtcgt attatcttgc tggaagacaa ttttctggtt gcgcacagcg
       61 gattgcattc gctcgatgtc gccagccgac acggccatat ccgctacaat cgtgatttcc
      121 gattgtggtg ccacgtattg atcgagccct gccacgatcg ccgggccgcg cccgttccaa
      181 ccgaggatca gcgtgcgttc gggtttggcg atactgcgcg gcgcgttatg gatcgcactt
      241 cgatcgatcg acaaacgccg atcgagattg tcccacgatc cgatcaggct tgacagctta
      301 atcgtgtcat catcttccga aatcgcgatc aactgatcgc ccgactggat ctgcgtacgc
      361 attggtggat tcaacaacgg gctaccatcg cgcggtcgca agccgatgat tgtcgagtct
      421 tcgtaagcca tcagggcatc gccgaatgtt ttgtccacca aggccggttc ttctttaaag
      481 tagatttcat cgccgccgaa atccagcaat tcggtataga caatactcaa acccgattgg
      541 cgtgaagtct gcacgatgat gcgcgagatc aaatcgccgg tgacgatcat ctctacttcg
      601 tctttaccca ccattcgcgc cactgccaga tttttgggat caccgatctc ggcgacaatg
      661 tgatacgggt cattacgccg attgggattg ttggtcaagg cgaggatcgt cttgataatc
      721 tcggaatccg gatcgctgct gtccgtcggc gccaggatga tgatcgatcg cgcggcgtgt
      781 ggattgacga tctctaaatc gctgatgtcg agcggactac ccgtgcggca aaccactttg
      841 gttttaccca gcgattcgat ctgcgtagcg atctcatctt ccatttcgac tttgtctttt
      901 tctgccagaa tgacaatgcg cggtttggct tggtttgcgt tggccgcgac caattccgaa
      961 atgatcgtga atattttggg cgaccaattc agaatcagcg tgtgcttctg ctcgatgaca
     1021 aacgaacggc ctttgcgcag ttcgtccatg cgattctcca gcccattgtt gatgattccg
     1081 atcaacgcac tgacgacaaa cacaccgccc agcgtcacgc ccagcatcac cacgcgccac
     1141 gcgatgcccg agtcgccgcc aaatgtgcct gaatcgagtg tgcgtaccag actcagccac
     1201 gcggcctcaa aaaaaccgac ttgcgtaccg tcatcttgtg taattcctcc ggcggcgatg
     1261 atcgccccgg ccacggcaat aataatcagc gagacaatcg ccaggccgat caacaacgcg
     1321 atcgagccgc gtgacatgat gttatcgaag cgatagcgca gtcgatctcg aaatgtgact
     1381 tgccgcatta tatttttctc ctcaccggga atgggctgcc cctgatgata ccgcaaacag
     1441 ccgtcaacgc aaaatcgatc gatgcatatg tcattgcgag ccgcgaagcg gcgaagcaat
     1501 ctcagccatt atcaagtgag attgcttcgt gggccaaacg gcttcctcgc catgacgtgc
     1561 gattgacgca aacgatgtag cggtattgta taatcacatt cgatcacgtt gataaggaaa
     1621 cttacatgtc ttcttcaacc gccgatgtcg ttatctgcgg cgcgggcatt tgcggaatcg
     1681 ccgccgcgta tcatttgacc gtcacgcaaa aaatcaaaaa tgtcgtgttg atcgacgagc
     1741 gcccgccctt gacgttgacc agcgacaaat cgaccgaggc gtatcgcaat tggtggcccg
     1801 gccccggcga tgcgatggtg cagttaatga atcgcagcat cgattggctg gaacgttggg
     1861 cggacgccac ccacaaccgc ttcgtgttga atcgacgcgg ctacgcttac ttcaccgccg
     1921 atccaaatcg cattcctgat ttcaaactcg cggccgctga agcagcgatg ttaggcgctg
     1981 ggccggtgcg cttgcatgtc gatcgatttt ctgatcgatc atatctgccg atcgatccgc
     2041 atcgctttgc tgatcaaccg atcgggtgtg atctggtgct cgatcgatct ctgatcctcg
     2101 atcggtttcc gtgcgtcaat gaaaatgttg tggcgctggt gcatcctcgc cgctgcggct
     2161 ggttcagcgg gcaaggactc ggcatgtatt tgttagaaca agctaaggcc catggcgcgc
     2221 gcttgatcga ggggaagatc gatcgggtgg atgtaaaatc aaatcgggtt cactctattc
     2281 attatgggct tgaattgatc gatacaaaca actttgtgat tgctgccggt ccttatctca
     2341 aagaagtcgg gcaattgatc ggcgtcgata ttccagtgtt taatgagctg catgtcaaga
     2401 tcgccttcaa agatcatctc ggcatcattc cacgcgatgc gccgctgatg atttggactg
     2461 atccgcaatt cctgccatgg acggctgaag agcgcgaggc gctccaagaa gatgaagata
     2521 cgcgctggtt gttgaatgaa tttccgggcg gcgtgcatct gcggcccgaa ggcggcggcg
     2581 agagccagat cgtgttgatg ttgtggccat atcacgtttc tccggttgag ccaaaatggc
     2641 cgccggaact cgatccgttt tttcctgatg ttgtcttgcg cggcctatcg acgatgatcc
     2701 ctgatatgaa aaaatatttt gataagaccc caaaatcttc atatgatggc ggttattaca
     2761 cgaagacacc agagaatcgg ccgcttgcca gcaaactgcc gatcgatggt gcgtttgcgt
     2821 gcggtgcagt ctctggttac ggcttgatgg cctcggcggc gatcggtgaa ttattggcgg
     2881 cgcaggtggc gcataccgaa ctgccaagtt atgctccggc gttctcactc gatcgatata
     2941 aagatcgagc ctaccagaaa cttttggaga attggggatc aagcggacag ttgtaaaaca
     3001 atgatcaatt atcaaggtgc aatgagcaaa gtgaaacggc gtgcttgaaa gtttcgttcg
     3061 aggtataatc aacctaggtt atccaggtct cattgggaga gatcgcaatg tgcgccaggc
     3121 atgaacacca accggggcga caatcgccgc gcaatttcat tatgaactgg aatcagacgc
     3181 tgccactgcg ggttaagatc agtaagacgc gaaagaattt atcgatccgt gtgatcaagc
     3241 gtcagacgtg ctgcgggcat gacggcgagt ctggctgttg aca
//
LOCUS       DASRTJ010000372         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_123301,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000372 DASRTJ010000000
VERSION     DASRTJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123301"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFF70_07505"
     CDS             complement(<1..295)
                     /locus_tag="VFF70_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44578.1"
                     /translation="MLQSLDMRYNAAMNSLGRDDDFIRRIVLPLILVITLAVFVMVPV
                     LAITWSQRPFIGAFIEQTGVTSQINGANWPARSLGLPGAAHILQIDGTAVQTAQ"
     gene            604..>2689
                     /locus_tag="VFF70_07510"
     CDS             604..>2689
                     /locus_tag="VFF70_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZY44579.1"
                     /translation="MRKLKKLLIVFILFGSICSAGVYAWLFVDLPSLDTLYQHAAATS
                     TKIVDRYGRLLYEIADPHRGKHTPVKLSDIPPYCQQATIATEDANYYSHPGVDVVGIL
                     RAVWINLQGGEVLSGGSTITQQLARNLLLSPEERSQRTITRKLRESILAWRITQQFSK
                     DDILDLYLNEVYYGNLAYGIEAAAQTYFGKPVGQLDLAECALLGGLPQSPATYDPLID
                     LKAALVRQATVLDLMLKQNDITPDQLSLAKAETLHFASVPFPIEAPHFVAYVRQWLED
                     RYGLEAIYTQGLVVTTTLDLDLQNTAQSIAQRQIANLKKDQPDQPGKNVNDAAMLAMN
                     PIDGEIYVMLGSPDYFDAQIDGAVNATIAHRQPGSSIKPLTYAAAFDPTAPDPLTPAS
                     MILDVRSSFPTREGDPYLPKNYDLTFHGPASARTALASSFNIPAVKVLQHVGLDRMIG
                     LARNIGITTFGQPDRYGLALTLGGGEVRLIDMVAAYSAFANGGHKIDPVAVLQVSDAT
                     GSVLYKASPQQGAQVLDARVAYLITDILSDNTARAPGFGEYSVLRLDRPAAAKTGTTT
                     DWRDNWTLGYTPDLVTGVWVGNADNKPMEHVSGVTGAGPIWHDFMLAALKGKPAQKFT
                     RPAGLIDFDVCATSGLLPTPYCPFTQREVFIQGTQPTQPDNLYRPIKIDVATGLPATA
                     DTPPDRVQTKVYL"
BASE COUNT          599 a          744 c          757 g          589 t
ORIGIN      
        1 cctgcgcagt ctgcacggca gtgccatcga tctgaagaat gtgcgccgcg ccgggcaagc
       61 ccagcgatcg agccggccag tttgcgccat tgatttgact ggttacgccg gtctgttcaa
      121 tgaacgcgcc gatgaaaggt cgctgcgacc aggtaatcgc gaggacgggc accatgacga
      181 agacggccag cgtgatgacg aggatgagcg gcaaaacaat tcggcgtata aaatcgtcgt
      241 cgcgtcccag gctgttcatg gcagcattgt agcgcatgtc aagtgattgg agcaaatttg
      301 actctctccc ctcgccacac gacgcatttg tttttgtcgt gaggagaggg gccgggagtg
      361 aggcgttggt tgaccgtagg gtgagcggcg gtagaatggt agcaacgatt ttgaatgctg
      421 aagcgtctag taaacgacgt cattgcgagc cgcaatagcg gcgcggcaat ctcaccttcg
      481 gtcgatcagg agattgcttc gtcgcgtcct tcgactccgc gttgcgcgct ccgctcagga
      541 tgtttttcgt aatgacggag cgacttttta tcattagtca tttttcattt gtcattgatc
      601 atcatgcgta aactcaaaaa acttctcatc gtcttcattc tcttcggatc gatctgctcc
      661 gccggtgttt acgcctggct gttcgtcgat ctgccatcgc tcgatacgct gtatcaacat
      721 gccgccgcaa cctcaaccaa gatcgtcgat cgatacgggc ggctgctgta tgagatcgcc
      781 gatccacatc gcggcaaaca tacgccggtg aagttgagcg acattccacc atattgccag
      841 caagcgacga tcgcgaccga agacgcgaat tattattcgc atcccggcgt ggatgttgtc
      901 ggcattttgc gcgcagtctg gatcaattta cagggcggcg aagtgctcag cggcggcagc
      961 actatcactc agcaactggc acgcaatctg ctgctctcac ccgaagagcg atcacagcgc
     1021 acgatcacgc ggaaactgcg cgaaagtatt ttggcgtggc gcatcacgca gcaattcagc
     1081 aaagacgata ttctcgatct ctatttaaac gaagtgtatt acggcaatct cgcgtatggc
     1141 atcgaagcgg cggcgcagac ctattttggc aagcccgtcg gccaactcga tctggcggaa
     1201 tgtgcgctgc tcggcgggtt gcctcaatcg cccgcgactt acgatccgtt gatcgatctg
     1261 aaagccgcgc tagtacgaca agcgaccgtg ctcgatttga tgctgaagca gaatgacatc
     1321 acgcccgatc agttatcgct ggcgaaagca gagacacttc actttgcatc ggtgccattc
     1381 ccgatcgaag cgccgcactt cgtggcgtat gttcgtcaat ggcttgaaga tcgatacggc
     1441 ctcgaagcaa tctatacgca gggcctcgtc gtgacaacga cgctcgatct tgatttgcaa
     1501 aacaccgcac agtcgatcgc ccaacggcag atcgccaatc ttaaaaagga tcagcccgat
     1561 cagccgggca agaatgtgaa tgacgccgcc atgctggcga tgaatccgat cgatggtgaa
     1621 atctatgtga tgctcggcag ccccgattat ttcgacgcgc agatcgatgg cgcagtcaac
     1681 gccacgatcg cgcatcgtca accgggatcg agtatcaagc cgcttacgta tgcggccgcg
     1741 ttcgatccga ctgcgcccga tccattgaca ccggcttcaa tgatcttgga tgtgcgatcc
     1801 tcttttccaa cacgcgaagg cgatccgtat ctgccgaaaa attacgatct cacttttcac
     1861 ggccccgcct cggcgcgaac agccctggcg tcttcattca atattcccgc cgtaaaagtt
     1921 ttacagcacg tcgggctgga tcgaatgatc gggctggcga gaaatatcgg catcaccacg
     1981 ttcggccagc ctgatcgata tggtttggca ctcacgctcg gcggcggcga agttcgattg
     2041 atcgatatgg tggcggccta cagtgcgttt gcgaacggcg gccacaagat cgatccagtg
     2101 gcggttttgc aagtgagcga tgcaaccggc agtgtgttgt ataaagcgtc accgcagcaa
     2161 ggcgcgcagg tgctcgatgc gcgcgtggcg tatttgatca ccgacatctt gagcgacaac
     2221 actgcgcgcg cgccgggctt cggcgaatac agcgtgctgc ggctcgatcg accggcggcg
     2281 gcgaagacgg gcacgaccac cgattggcgc gacaactgga cgctgggcta cacgcccgac
     2341 ctggtgaccg gtgtgtgggt tggcaatgcg gacaacaaac cgatggagca cgtcagcggc
     2401 gtgacgggcg cgggaccgat ctggcacgat ttcatgttag ccgcattgaa aggcaagccc
     2461 gcgcaaaaat ttacgcggcc tgcgggtttg atcgatttcg atgtgtgcgc gacttcagga
     2521 ttattaccaa caccatattg tccattcact cagcgcgaag ttttcattca aggcacgcag
     2581 ccgactcagc ccgataattt atatcggccg atcaagatcg atgtggcaac tggtttacct
     2641 gcaactgctg atacgccgcc cgatcgagtg cagacgaaag tgtatttag
//
LOCUS       DASRTJ010000373         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_98405,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000373 DASRTJ010000000
VERSION     DASRTJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98405"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFF70_07515"
     CDS             complement(<1..367)
                     /locus_tag="VFF70_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY44580.1"
                     /translation="MDFNLTASQLEIQKRARDFAQNRIAPLAREMDEKGEMPLALVRE
                     MSGLGFLGGPLPAAYNGGGWDAVSLALCYEELGRADSSVRGLMTVHTSLVSQCILQWG
                     TADQKNKYLPRLARGESIGC"
     gene            complement(540..944)
                     /locus_tag="VFF70_07520"
     CDS             complement(540..944)
                     /locus_tag="VFF70_07520"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZY44581.1"
                     /translation="MHKTSVNPAILVKPSGYAHGIVTHGGRSLFLAGQTGMDQSGKIA
                     TPNDIVAQFTQALNNIKAVIDEADGEMTDIVKLNLFVTDKAAYQANLKPIGASYRDFF
                     GRYYPAMTLVEVKSLFDDQAMIEIEGIAMLKD"
     gene            complement(953..1741)
                     /locus_tag="VFF70_07525"
     CDS             complement(953..1741)
                     /locus_tag="VFF70_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZY44582.1"
                     /translation="MTDFDYTVSNSIATITLNRPEVLNALTLEIYAQLRDLFEALRDD
                     DKVKVVIITGAGRAFCSGGDVHTIIGEVLKRDMHGHLEFCRMTGHLVRNMRLLPKPII
                     AAINGMAAGAGAVIALASDIRVVADSAKFAFLFTRVGLTGADMGAAFLLPRVIGQGRA
                     MELLMLGDEVDAATAERYGLANRVLPLDQVLPAAQQLAERLAAGPSYSIGMTKLMVNN
                     EWTMDINSALEAEAQAQALMMMGEDHRIFYEAFKEKRKPQFKGK"
     gene            complement(1905..2678)
                     /locus_tag="VFF70_07530"
     CDS             complement(1905..2678)
                     /locus_tag="VFF70_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY44583.1"
                     /translation="MDTQKIAWVTGAGKGIGEAIALALGASGLKVAVSARSAADIDRV
                     AATLREKGGESIAVVCDVTQPESIRVAVAQIQNSLGPIAILVNGAGAAESQKFLGHDD
                     ALWRRMIDVNLNSVYYVCKEVAPLMVEAKWGRIINIASIASKVGGKYVAAYTAAKHGV
                     LGLTRALALELVAHNITVNAICPGYVDTPLTDQSIAAITRRTKLSENEARAALEKISP
                     QNRLITSAEVAHVALMLVHDNARGITGQAINVDGGTVMF"
     gene            complement(2681..4228)
                     /locus_tag="VFF70_07535"
     CDS             complement(2681..4228)
                     /locus_tag="VFF70_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HZY44584.1"
                     /translation="MKINILGAGPAGLYFAILIKRIDPAHEIDVFERDGEHATFGWGI
                     VFSETTLNNFEQHDPETYAEFVKAAQNWQAVVVKHKGETITIGGNPISGVARLKWLNI
                     LHQRCRDLGIHLHFNLNVTDVNSYRDCDLLVGADGANSLVRKTYEAFYMPSIEVRQNK
                     FIWLGTEAPFNGLTMIFKQTEVGLFISHSYQFSPTHSTFIVECPPETWLKAGFEKISD
                     AETCVYLANIFKSDLGGKPLLSNNFVKWNNFPLVKVRRVYHQNVVLLGDAMHTAHFSI
                     GSGTKLAMEDSIALADAFARCRTVDAALPEFQRVRKPVIDRFQTAALKSLAWLEDVQN
                     YLHLDPIPFTYKLMTRSKRVGFARLQRADPGFAARYEAWRKAQKQSGPIPDEFLDLFR
                     KKTFGHLSTLMPNGTPHVTSVYVDYDGQYILINSARGRVKDINMAERPNVAIQIPDPD
                     NSDRYIAVRGKVVEITEEGADAHLDKLAQRYLDKDFYPPSMRYPGEVRCIYKIEPKHV
                     TTWNPFG"
     gene            complement(4516..>5340)
                     /locus_tag="VFF70_07540"
     CDS             complement(4516..>5340)
                     /locus_tag="VFF70_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44585.1"
                     /translation="DWIDLKADGRTILYTSEGPEILAYDVLSKTQKPDFASGLQAPCF
                     AVRIRPNTEVMVACSQGVYRLSSSGGISHTYPITSLNETDPFGLFALNLDSDGTSFWT
                     AGFSSGKVYKVDIASGTVLTSFNAHPLADSMAHAYIVGLAVYGEMQVGRPNLTLVETV
                     GTSPGSCAGTREINVAANTPVYYCYTIKNTGVVTLTTHALTDTVHGVIFSNQAINLSP
                     GATAFVTRTATINATTINIATWTGVNGGQSASAFDFTRVNVQLLLSNKVFLPVVLK"
BASE COUNT         1155 a         1548 c         1401 g         1236 t
ORIGIN      
        1 agcacccgat cgattcaccc cgcgccagcc gcggcagata tttatttttt tgatctgccg
       61 tgccccattg caaaatgcat tgtgagacta aactcgtatg caccgtcatc aagccgcgca
      121 ccgatgaatc cgcgcggcca agttcttcat agcataaggc cagcgagacg gcatcccagc
      181 caccgccgtt ataggctgcg ggcagcgggc cgcctaaaaa ccccagacca ctcatctcgc
      241 gcacaagtgc gagcggcatc tcgcctttct cgtccatctc gcgcgccagc ggcgcgatcc
      301 gattctgcgc gaagtcccgc gcgcgttttt gaatttctaa ttgacttgcc gttagattaa
      361 aatccatttt gtcgtaacta ctccgcagtc atgcccgaat gcttgtatcg ggcatccagc
      421 ttgaccatta gtgagtgacg ctgatagggt ctctggattg ccgcctaaag catgcgggaa
      481 tgaccctcgg catctaggct gagtccttac agtttatctc caaccaattg gaaaccactc
      541 taatccttca gcatcgcaat accttcaatc tcaatcatgg cctgatcatc aaacagactt
      601 tttacttcga ctaacgtcat cgcgggataa taacgcccga agaagtcgcg atagctcgcg
      661 ccgatcggtt taaggttcgc ttgatacgcg gccttgtcgg tgacgaacaa attcagtttc
      721 acaatatccg tcatctcacc gtccgcttca tcgatcacag ctttgatgtt gttcaacgcc
      781 tgtgtgaact gcgcgacgat atcgttgggt gttgcgatct tgcccgattg atccatgccc
      841 gtttggcccg cgagaaaaag cgatcgaccg ccgtgcgtga cgatgccgtg ggcatagccc
      901 gatggtttga cgagtatggc cgggttgacg cttgttttgt gcatcttctc gatcacttcc
      961 ccttaaactg tggcttgcgt ttctctttga atgcttcata aaatatgcgg tgatcttcgc
     1021 ccatcatcat taatgcctgg gcttgcgctt cggcttcaag cgcagagtta atatccattg
     1081 tccattcgtt gttcaccatc agcttcgtca tgccgatcga gtagctcggg ccggcggcca
     1141 gccgctcggc gagttgttga gcggccggca gcacctgatc gagcggcagg actcgattcg
     1201 ccaagccgta gcgttcggcg gtggcggcat cgacctcatc acccagcatc aacaactcca
     1261 tggcgcgccc ctggccgatc acacgcggca gcaaaaaggc cgcgcccata tcggcgccgg
     1321 tcagtccgac gcgcgtgaag agaaaagcga acttggccga gtcggcaacc acgcgaatgt
     1381 cggaagccaa cgcgatcacc gcgcccgcgc ccgccgccat gccattgatc gccgcaatga
     1441 tcggtttggg cagcaagcgc atattgcgca ccagatgtcc ggtcatccga caaaattcca
     1501 aatgcccgtg catgtcgcgc ttcaacactt cgccgatgat cgtgtgcacg tcgccgcccg
     1561 aacaaaaggc gcggccggcg ccggtgatga tcacgacttt tactttatcg tcgtcacgca
     1621 gcgcttcaaa cagatcgcgc agttgagcgt agatctccaa cgtcagcgcg ttcagcacct
     1681 cgggccggtt gagggtgatc gtggcgatcg agttcgatac cgtgtaatca aaatcggtca
     1741 tggcattcct cattgagtaa gagcgcggtt atctcgctgt taaatttatt cactcatgtt
     1801 acgcactgtc acacccgcga aggcaggtgt ccagccgttt ttctctagat tcccgctttc
     1861 gcgggaatgg cgattaacct gccacgcgta aggtgacttt tttatcaaaa catcaccgtg
     1921 ccgccatcaa cattgatcgc ctggccggtt atgccgcgcg cgttgtcgtg aacgagcatc
     1981 aacgctacat gcgcaacttc agccgaagtg atcaaacggt tctgtggact gattttttcc
     2041 agcgccgccc gcgcctcgtt ttcagaaagt tttgtgcggc gcgtgatcgc ggcgatggat
     2101 tgatcggtga gcggcgtatc gacgtagccg ggacagatgg cattgacggt gatgttgtgc
     2161 gccaccagtt caagcgcgag cgcgcgcgtc agtccgagca caccgtgctt cgccgccgtg
     2221 taggccgcaa catatttgcc accgaccttc gaagcgatcg aagcgatgtt gatgatgcgc
     2281 ccccacttgg cctcgaccat cagcggggca acttctttgc acacgtaata caccgagttg
     2341 agattcacat cgatcatgcg ccgccacaaa gcatcatcgt gtcccaagaa tttttgtgac
     2401 tcggcagcgc ccgcgccatt gaccagaatc gcgatcggac ccagcgagtt ttgaatttgc
     2461 gcgacggcaa cgcggatcga ttcgggttgt gtcacatcgc acaccaccgc gatcgattcg
     2521 ccgccttttt cgcggagggt ggcggcgacc cgatcaatat cggcggccga gcgcgcagag
     2581 accgcgacct tcagcccact cgcgccaagc gccagagcga tcgcctcgcc aatgccttta
     2641 cccgcgcctg ttacccacgc aattttctgt gtgtccacgt ttatccgaaa ggattccaag
     2701 tcgtgacgtg cttcggttcg attttgtaaa tgcagcgcac ttcgccgggg tagcgcatac
     2761 tcggcggata aaaatctttg tcgagatagc gctgggccaa tttatcgaga tgcgcatccg
     2821 cgccctcttc ggtgatctcg acgactttgc cgcgcacggc gatatatcga tccgaattat
     2881 ccggatcggg aatctggatc gccacattgg gacgctctgc catattgata tcttttacac
     2941 ggccgcgcgc cgaattgatg aggatatact gaccatcata atccacatat accgaagtga
     3001 catgcggcgt cccgttcggc atcagcgtgg agagatgtcc aaaagttttt ttccggaaga
     3061 gatcgagaaa ctcatccggg atcggaccgg attgcttctg cgctttgcgc cacgcctcgt
     3121 agcgcgcggc gaagcccgga tcggcgcgtt gaaggcgcgc gaagccgacg cgcttgctgc
     3181 gcgtcatcaa tttgtacgta aacggaatcg gatcgaggtg caaataattt tgcacatcct
     3241 cgagccacgc taaacttttt aaggccgccg tctgaaaccg atcgatgacc ggcttgcgaa
     3301 cacgttgaaa ttccggcagc gccgcatcga ccgtgcggca gcgcgcaaag gcgtccgcca
     3361 acgcgatcga atcttccatc gccaactttg tgcccgaccc gatcgagaaa tgcgcggtgt
     3421 gcatcgcgtc gcctaacaac acaacgttct gatgatacac ccgccgcact ttgactaatg
     3481 gaaagttgtt ccatttcaca aaattattgg agagcagcgg cttgccgccg agatcagatt
     3541 tgaaaatatt cgccagatat acgcacgtct ccgcgtccga tattttttca aagcctgctt
     3601 tcaaccacgt ttcgggcgga cattcgacga tgaaggtgct gtgcgtcggg ctgaactgat
     3661 acgagtgcga gataaacaaa ccaacttcag tttgtttgaa gatcattgtc aggccgttga
     3721 acggggcctc ggtccccaac catataaatt tattctgccg cacttcaatc gacggcatgt
     3781 agaacgcttc gtatgttttg cgcaccaggc tgttcgcgcc gtctgcgccg accagtagat
     3841 cgcagtcacg ataggaattc acatcggtga cgttaaggtt aaagtgcagg tggatgccca
     3901 gatcgcggca gcgttgatgc aaaatattca gccacttgag gcgagccacg ccggagatgg
     3961 gattgccgcc gatagtgatg gtttcccctt tgtgcttaac gacgacggcc tgccagtttt
     4021 gagcagcctt gacaaactcg gcgtacgttt cgggatcgtg ctgctcgaaa ttattcagcg
     4081 tggtttccga aaagacaatg ccccaaccaa acgtagcatg ttcgccatcg cgctcgaaga
     4141 catcgatctc atgcgccgga tcgatccgtt tgatcaagat cgcaaaatac agcccggcgg
     4201 gaccggcgcc aagaatgttg attttcattt catttcaccc ggttctccgg catctgcacc
     4261 gccgtcgctt ctcgccacgc tcatcgcgac gctctcaccc gacccaaatc tggctgtcag
     4321 tatatgacga caattcatca aagtcaattt gcatgttgcg attgggcgta gtccgaagtt
     4381 tagcatccct caaccgagct agccccgctg atcgatcggg cttagcgatc accacaaaaa
     4441 tatggctaaa acaaaacgcc cgatcaattt ggcttgatcg ggcgtttgtg atctgataag
     4501 gagattaact gtgtgttact tcaagaccac cggcaggaat actttattgg acagcagcag
     4561 ctgaacgttt acccgcgtga agtcgaaggc gctggcgctc tggccaccgt tcacgcccgt
     4621 ccacgtcgct atattgatcg tcgtcgcgtt gatagtcgcg gtacgtgtaa caaatgccgt
     4681 cgcgccgggc gaaagattga tcgcctggtt cgagaagata acaccgtgca ccgtatccgt
     4741 cagcgcatgt gtggtcagcg tgaccacgcc tgtgttcttg atcgtgtagc agtaatacac
     4801 cggcgtgttc gcagccacat tgatctcgcg agtgcccgcg cacgatccgg gcgatgtgcc
     4861 gactgtttcc accagcgtca ggttcggccg tccgacctgc atctcgccat acaccgccag
     4921 tccgacgata taggcatggg ccatgctatc cgccaatgga tgcgcgttaa atgaagtcag
     4981 cactgttccc gatgcaatat ccactttgta aaccttgccg ctgctgaagc cggccgtcca
     5041 gaacgaagtg ccgtcactat ccagattgag cgcgaacaat ccgaaggggt ctgtctcatt
     5101 gaggctggtg atcgggtaag tgtgtgagat cccgccgctg ctgctgagcc ggtagacacc
     5161 ctggctgcac gcaaccatga cctcagtatt tgggcggata cgcactgcga aacagggtgc
     5221 ttgcaagcca ctcgcaaagt ctggcttttg tgtcttcgac agcacatcgt acgccagaat
     5281 ttccggtccc tccgatgtgt agaggatggt gcgtccgtcg gcttttaagt cgatccagtc
//
LOCUS       DASRTJ010000374         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_98440,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000374 DASRTJ010000000
VERSION     DASRTJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98440"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>2232)
                     /locus_tag="VFF70_07545"
     CDS             complement(1..>2232)
                     /locus_tag="VFF70_07545"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HZY44586.1"
                     /translation="GIAHPPGYPLYTLIGFLFTRLFVSPAFAMNLLSAIIASITLVIV
                     SKTVRLLTRSVLAGLLAASILGLSTTFWSQATTANIRMPTALFTALCVYILVSWQLTI
                     SVQQSANLAIPSSRRLAFSPTLTLFAITFSLALSHHLSIIFPGIFFIIYIFLIDRSLI
                     KQPRRWIKPAIVFLIALLVLAYLPIRGATGGTLSDGESTTYLVQPDKFLDHVLARGFE
                     GDFFYFLNTRSDLLLDRVALIPTLFNFQFNWLVAILALIGSIRLLLRDRKLALMLLGG
                     IGLHTFITITYRAPQTVEYMLPAYVLLAIVVGYGLGDFGMQIADFGRFRIPHSAFRII
                     RWILIGISSLAAILLFLNHFPSYNWLHQPADTREYAATLLNDAPPNAIILSNWHWANP
                     MWYLQQVEGLRSDVTVQYVYPRGEPLATSWLTGIDAGLKQNRPVIVDMYFRSQFDASQ
                     YFFDPIASEAFEVRKSPQAVMPASFVTSSSDFENKFRLSGYSVPHGSTQPGEPAIVTL
                     AYRVESQPDRDYSFFVHLIDSSGNVIGQMDQTIPTTRYHVGDVIVARFFVAPLPTVQT
                     GEYQLMAGIYSVGGDGKIVALKSAGSEQVSVTNLKVDPPDRLNNPSGIDLSSGIVFIN
                     STSSTSGELHPGDRLTLDLHFIATRPIQRDIVVSVQMAGSNWRAIDDSIPALGAIPTL
                     KWIAGSQIDDPHVLIVPQKAAGQATVTLSLYDAFTQKPLTVLDADLIKQGPTIPLGTW
                     TIK"
BASE COUNT          600 a          531 c          632 g          469 t
ORIGIN      
        1 tcatttaatc gtccacgtcc ccaatggaat cgtcggacct tgtttgatca agtcggcatc
       61 caacacggtc agcggttttt gagtgaaggc atcatacaaa ctcaacgtga ccgtcgcttg
      121 acccgcagct ttctgcggaa caatcaatac atgcggatca tcgatctgtg atccggcgat
      181 ccatttcaat gtcggaatcg cgccaagcgc aggtatgctg tcatcgatcg cgcgccagtt
      241 cgatccagcc atttgcacgg acacgacgat gtcgcgttga attggccgcg tggcgatgaa
      301 gtgtagatcg agggtaagcc gatcgccggg atggagttcg ccgctcgtcg atgaagtcga
      361 attgataaac acaatgccgc tggataaatc tatacccgat ggattgttca agcgatcggg
      421 cggatccact ttcaagttag tcactgaaac ctgttcactc cccgccgatt tcaacgcaac
      481 aatttttcca tcgccgccaa ctgaatagat gcctgccatc aattgatatt cacctgtttg
      541 aacagtcggc aacggcgcga cgaagaaccg cgcgacgatc acatcgccga cgtgataacg
      601 cgtcgtcggg atcgtttgat ccatttggcc gatgacgttt ccgcttgaat cgatcagatg
      661 cacgaagaac gagtaatctc gatcgggttg ggattcgacg cgataggcta acgtgacaat
      721 cgctggttcg ccgggctgag tcgatccgtg gggtacggaa tagccgctga gccggaattt
      781 attttcaaag tcactgctgc ttgttacaaa cgaggctggc attacagcct gcggtgactt
      841 ccgtacttca aaggcttcgg acgcgatcgg gtcaaagaaa tattggcttg catcaaattg
      901 tgatcggaaa tacatatcga cgatcactgg tcgattttgt tttaaaccgg catcaatgcc
      961 agttagccat gaagtcgcca gcggttcacc gcgcggataa acatactgca ccgtcacatc
     1021 gctgcgcagg ccttcgactt gctgcaaata ccacatcgga ttggcccaat gccaattgga
     1081 taagatgatc gcgttcggcg gcgcgtcgtt caagagcgtg gctgcgtact cgcgcgtgtc
     1141 agcaggctga tgcagccaat tatatgaagg gaagtggttg aggaagagga gaatggcggc
     1201 caaactgcta atgccgataa gtatccagcg aataatgcgg aatgcggaat gtggaatgcg
     1261 aaatctgccg aaatccgcaa tctgcattcc gaaatcgcca agcccatagc ccactacaat
     1321 tgccaacagc acatacgcgg gcagcatgta ctcgacggtc tgcggggcgc gataagtgat
     1381 cgtgataaag gtgtgaagtc cgatcccgcc gagcagcatc agcgccagtt ttcgatcgcg
     1441 cagcaacaag cggatcgatc cgatgagcgc caagattgcg acaagccaat tgaattgaaa
     1501 gttgaacagc gtcgggatta gcgcaacccg atcgagcagc aagtccgatc gagtgttcag
     1561 gaagtaaaag aagtcgcctt cgaagccgcg cgctaaaaca tgatcgagaa atttatctgg
     1621 ctgaacaaga tatgtggtcg attcgccatc gctcaatgtg ccgccggttg cgccgcgaat
     1681 cggcaagtaa gccagaacca gcaatgcaat caagaatacg atcgcaggtt tgatccagcg
     1741 ccgcggttgc ttgatgagcg atcgatcgat caagaaaatg tagataatga agaagatacc
     1801 ggggaagata atagagagat gatggctgag agcgagtgag aaggtgattg caaagagagt
     1861 gagagtagga gagaaagcga gacggcgaga tgacgggata gcaagatttg ctgattgctg
     1921 aacactgatg gttaattgcc agctaactag aatgtaaacg cacaacgcag tgaagagcgc
     1981 ggtcggcatg cggatgttgg cagtcgtagc ttgcgaccag aaggtcgttg agagaccgag
     2041 aattgatgcg gccaataagc ccgctaagac cgatcgggtt aatagacgaa ccgttttgct
     2101 gacgatgacc aaagtgatcg aggcaatgat cgcgctgagg aggttcatcg cgaaagccgg
     2161 cgagacaaat aggcgggtga acaaaaaacc gatcagcgtg tagagcggat aaccgggcgg
     2221 gtgcgcgatg cc
//
LOCUS       DASRTJ010000375         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_23308,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000375 DASRTJ010000000
VERSION     DASRTJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23308"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1095)
                     /locus_tag="VFF70_07550"
     CDS             complement(247..1095)
                     /locus_tag="VFF70_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44587.1"
                     /translation="MSQPADLIPTIEAELLEEIARPSRFSQRLRRYLPAIALFIIGLI
                     IWEVIVVVFNIQGFLLPRPTVIASRFVEQFSIIVSDGLFTMREAVGGFFIGSILGMLV
                     ALATSRWASISEGVMPFAIAINSTPIVALAPIMNNWFGITNPIAKMSIVAVIVFFPVM
                     INVVRGLTQVNPGAIELMKSYAASDFSVLFKTRIPNALPYLFSALKVASTLSVIGAIV
                     SEYFGGSREALGVYISQQAALFHFAEAWSAIIVACLMGIAFYVIILLVERWVMPWHVS
                     LRSTQE"
     gene            complement(1098..1883)
                     /locus_tag="VFF70_07555"
     CDS             complement(1098..1883)
                     /locus_tag="VFF70_07555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44588.1"
                     /translation="MNSSQPIVSIRGVAKVFNQNSNNSVKALDNINVDIQPGEFVSLL
                     GPSGCGKSTLLRLVGDLTPPSAGSIRIKDKTAHQARLDRDYGMVFQQATLYDWRSVGK
                     NVQLPLEVMNFPPAKRETRTLEMLKLVQLEEFSQHYPWQLSGGMQQRVAIARALAFEP
                     SILLMDEPFGALDEMTRERMQLEVLRIWSQTKTTVIFVTHSIPEAVFLSNRVVVMSAR
                     PGRVTGVIDIDLPQPRDVETREHPHFFEKITEVREMLRKGEKV"
     gene            complement(1973..2881)
                     /locus_tag="VFF70_07560"
     CDS             complement(1973..2881)
                     /locus_tag="VFF70_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44589.1"
                     /translation="MATAPIDSELIAPQPQRLPGWLRKTLVFFTILIALCLLWEGYKA
                     IGAATGDKIPFTDTALPVRSDDSSMPHIANIVGALFLPAQRGNDTTVISILFQSALFT
                     WREAIVGFLIGSVIGFMLGVMFSRWQLLERGLMPYVVASQTIPLLAIAPMIVIWGSRL
                     GWDAWVAVTVISAYLTFFPVTINTLRGLRSPDPTAVELMRSYASSEWQLLWKLRVPAA
                     LPYIFTALKISATASVIGAVIGELPSGIADGLGRVLLTYSYYYISGPEKLYAAIIMAA
                     LLGIAFTLIIILIEKLILSPHRRIVE"
     gene            complement(3001..>3324)
                     /locus_tag="VFF70_07565"
     CDS             complement(3001..>3324)
                     /locus_tag="VFF70_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY44590.1"
                     /translation="TWSEYAQRWGSLQPHDRIDQALEDMAQIHPQIREEFEVGASKMW
                     HDDEFAGGAFALFDPGQQTLLYNEIISPEGRIYFAGEHASLAHAWIQGAIESGLRAAM
                     QIHSV"
BASE COUNT          814 a          915 c          895 g          700 t
ORIGIN      
        1 agcggtcaag acaaccgtca agacggcaaa caaactgata acacgcatta acttcgactt
       61 catggatttc tcctcctgag aatgggtatg gtgaatttgg atcgacttcg gttcaatggt
      121 ggatattcga tcagggtttg atctcgatcg tgctgcatca cctccttggt ccggtgatcc
      181 tcccacaggt tcagagttaa ttactcgttg atcattgcta aacctgccgg agaattgaat
      241 acccgattat tcttgtgtgc ttctgagtga tacgtgccac ggcatcaccc agcgctcgac
      301 cagcaaaatg atcacataga acgcgatgcc catcaggcag gccacgatga tcgcgctcca
      361 cgcttcggca aaatgaaaca gcgcggcttg ctgagagatg tacacgccca acgcttcgcg
      421 cgatccgccg aagtactcgc tcacgattgc gccgatgaca ctgagcgtgc tggcgacttt
      481 gagcgcgctg aacaaatacg gcagcgcatt gggtatgcgc gtcttgaaca acacactgaa
      541 atcgctggcc gcataggatt tcatcaattc gatcgcgccc ggattgacct gcgtcaagcc
      601 gcgcacgacg ttgatcatca ccggaaagaa aacgatcacc gccacgatgc tcatcttagc
      661 aatcgggttc gtgatgccga accagttatt catgatcggc gccagcgcca cgatcggagt
      721 ggagttgatc gcaatcgcaa acggcatcac gccttcgctg attgatgccc agcgcgatgt
      781 cgccaatgct accagcatac ccaagatcga tccgatgaag aatccgccga cggcttcacg
      841 catggtgaac agtccgtcag agacgatgat cgaaaattgc tcgacaaagc gtgaagcgat
      901 gacggttggt cggggcagca agaatccttg aatgttaaac acgacgacga tcacttccca
      961 aataatcaaa ccgatgatga agagagcgat ggccggcaaa taccgacgca gacgctggct
     1021 aaaacgcgac gggcgcgcga tttcttcgag cagttctgct tcgatcgttg gaatcagatc
     1081 ggcgggctgg ctcatcgtta aaccttttcg cctttgcgca gcatttcgcg cacttcggtg
     1141 atcttttcaa agaagtgcgg gtgctcgcgc gtttcgacat cgcgcggctg cggcaaatcg
     1201 atatcgatca cgccggtaac gcggccgggc cgcgccgaca tcacgaccac gcggttcgat
     1261 aagaacacgg cttcgggaat gctgtgcgtc acgaagatga ccgtggtttt agtttgactc
     1321 caaatgcgca gcacttccaa ctgcatgcgc tcgcgggtca tttcgtccaa cgcgccgaac
     1381 ggttcatcca tcaacaagat cgacggctcg aaagccagcg cccgcgcgat ggccacgcgc
     1441 tgctgcatgc cgcccgacaa ctgccacgga taatgttggc tgaattcttc caactgcacc
     1501 agcttcaaca tttccagtgt gcgcgtttcg cgcttggctg gcggaaagtt catcacttcc
     1561 aacggcagct gcacattctt gccaaccgat cgccaatcgt acagcgtggc ctgttgaaag
     1621 accataccgt agtctcgatc gaggcgggct tgatgtgccg tcttgtcttt gatgcggatc
     1681 gatccggctg atggcggtgt gagatcaccg accaatctca acaacgtgga tttgccacag
     1741 cccgacggac ccagcagcga gacgaactcg ccgggttgaa tatcgacgtt gatgttgtcc
     1801 agcgccttga cgctgttgtt gctattttga ttgaagactt tcgctacgcc acggatcgaa
     1861 acgataggtt gggagctatt caatgagttg actcctcgcg aattagaaaa tgcatcatgc
     1921 acagcgcatc attgaaatca atcaccgtgc atcaggcctt aggaattatg cattactcaa
     1981 cgattcttct gtgtggcgat aaaatcagtt tttcgatcaa gatgatgatc aaagtgaagg
     2041 caatgccgag cagtgcggcc atgatgattg cggcatacaa tttttccggg ccactgatgt
     2101 aatagtaact gtaggtcagc agcacgcgac ccaatccgtc tgcaatcccc gaaggcagct
     2161 cgccgatgac cgcgccaatg acactggccg tcgccgaaat tttgagcgcc gtaaaaatgt
     2221 agggcagggc cgctggtaca cgcagcttcc ataatagctg ccattcactt gaagcgtacg
     2281 atcgcatcaa ctcaaccgca gtcggatcag gcgagcgtaa tccgcgcaaa gtattaatcg
     2341 tgacgggaaa gaacgtgaga tacgccgaga tgaccgtgac cgcgacccac gcatcccagc
     2401 cgaggcggct gccccaaatg acgatcatcg gcgcgatggc gagcaacgga atggtttgcg
     2461 atgcgacgac atagggcatt aagccgcgct ccagcaactg ccatcggctg aacattacgc
     2521 cgagcatgaa gccgatcaca ctgccgatca agaaaccgac gatcgcttcg cgccaggtga
     2581 ataaggcgga ttgaaacaga atggagataa cggtcgtatc attgccgcgc tgcgccggga
     2641 gaaagagtgc gccgacgatg ttggcgatgt gcggcatgga gctgtcatcc gatcggacgg
     2701 gcagggcagt atcggtgaac ggaatcttat cgccggtcgc tgcgccgatc gctttgtaac
     2761 cttcccacag cagacacaac gcgatcaaga tcgtgaagaa aacgagcgtc ttacgcagcc
     2821 agccgggaag tcgctggggt tggggagcaa tcagttccga atcaatcggg gcggttgcca
     2881 ttggacactg attaaactct gaatctacaa aagagtcaag acaataatca atctgtaaag
     2941 catcctgagc ggagcatcga actcgaagag atcgatgctc cgctcagggt gcttacatcc
     3001 ctacactgaa tgaatctgca tcgcggcgcg caggccggat tcgatggcgc cttgaatcca
     3061 cgcgtgcgct aacgacgcgt gctcgcccgc gaaatagatg cggccttccg gcgagatgat
     3121 ctcgttataa agcagcgttt gttgtccggg atcgaatagc gcgaatgcgc caccggcaaa
     3181 ttcatcatcg tgccacatct tcgaagcgcc gacttcgaac tcttcgcgga tctgcggatg
     3241 aatctgtgcc atgtcctcca gggcctgatc gatccgatca tgtggctgaa gcgaacccca
     3301 gcgctgcgca tactcagacc aagt
//
LOCUS       DASRTJ010000376         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_23325,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000376 DASRTJ010000000
VERSION     DASRTJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23325"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1248)
                     /locus_tag="VFF70_07570"
     CDS             complement(127..1248)
                     /locus_tag="VFF70_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY44591.1"
                     /translation="MNKIKFSARSITISIVLILSIGCSSNPPAPTSAAFTQTPRSIVP
                     TTTAIPTSTSTPTITPTPTPVPNPLQIDVMRQQEYPGSDITIEQELPNGSNYKQYIAS
                     YLSEGLKIYALLTVPLGQKPDTGWPVIVFNHGFIPPQTYRTTERYVAYVAGFARSGYI
                     VIKPDYRGHGSSEGEATGGYGSPDYTIDVLNALASIKKYPDADPNRIGMWGHSMGGSL
                     TLRAMVISQDIKAGVIWGGVVASYADLLSKWHRPRPGETPTPASETSSQGRRWRNNLI
                     TEYGSPEENPDFWNSISPIDYVSDVSGPIQLDHSTTDEEVPYAFSVSLNQALLAAGKT
                     VEFYSYEGDNHNIANNFGLAMSRSIKFFDKYVKNIGPLG"
     gene            1485..4304
                     /locus_tag="VFF70_07575"
     CDS             1485..4304
                     /locus_tag="VFF70_07575"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZY44592.1"
                     /translation="MKEQETLWHSRSIAHVASELGTEPTRGLTSEEAAQRLLRLGPNE
                     LREKPSTPFWKLVLAQLNSFVVILLIVASIISALLGDYVEAAAIMAIVILNAVLGVVQ
                     ESRAEAALAALKKMAAPEAEVIRDGHRQKISSRELVPGDVVLIESGNYIPADLRLVDS
                     VNLKIDESALTGESNAVEKRSADTLAEHVPLADRRNAAYMSSLVTYGRGKGIVTGTGM
                     DTQIGLIAQMLQSFEEEPTPLQRKLDQLGKTLGTAAVFLCLIVFVVYVIRATNFSVLF
                     APDGGLGAYFQAFKAKLAEAFLIAVSLAIAAVPEGLPAVVTISLALGMREMIRRHALI
                     RKLSAVETLGSATTICSDKTGTLTQNEMTAVQLWVHHTEVLIEGRGYQPNGSFLRPDR
                     RDFNARDDVEVAGLLWAAALANDAELEPTGEAQGQQTYRMVGDPTEGALIVAAAKADL
                     WRRELEQDYPRVAEVPFDSERKLMSTIHGLRHVTEDDGSPFFPGEIGYVVCVKGAPGL
                     LLERSTYLLHRKGPLPIEDHDRAAIDESISRMGSQALRVLGVAYRKLDRLPSSIEAEE
                     IEQDLIFIGLIGMIDPARPEVKPAVQKARQAGIRTVMITGDHPDTARAIASEIGILRP
                     TGQLHTGVEIEAMSDDQLKAVVPDTDVFARVSPQHKVRIVEAFRARHHVVAMTGDGVN
                     DAPALKRADIGVAMGISGTDVTKETADMVLTDDNYVSIVGAVEQGRIIYSNIRKFVFY
                     LLSCNVAEVAIILIAAIAGYPPPLTAIQLLWLNLLTDGAPALALGMEKGDPDIMQQPP
                     RPSREPIINRIMVIRIAIMTVVLTAVVLTAFLIGLNEHGKDLAETMAFATLALAELPI
                     AFTSRSERYPVFRLGFFSNKWMPRAIALSVVLTLAVIYVPFLNKPFNTVPLTLQQWEV
                     IIPLILLPAIVAEISKYFVRRSEHH"
     gene            complement(4440..5147)
                     /locus_tag="VFF70_07580"
     CDS             complement(4440..5147)
                     /locus_tag="VFF70_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44593.1"
                     /translation="MTEKKRWIVVVIFSLAMAWMESATVTYLRTLVGRIDPYQARPLP
                     ISVGLGQAEIVREAATLIMLFTVGWLAGRSRRSRLGYMLIAFGIWDIFYYVFLAMISG
                     WPHSIFDWDVLFLIPLPWWGPVIAPALIALMMVIGGTLVRQPDQPDRAIWPSRGAWLM
                     NIGGVFVALYAFMADTLRAASEGLQAVRDVLPTSFNWPLYIIALVLMAAPIIDLTRKI
                     WGVSRQRTAQKFDLINH"
     gene            complement(5242..5601)
                     /locus_tag="VFF70_07585"
     CDS             complement(5242..5601)
                     /locus_tag="VFF70_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44594.1"
                     /translation="MNTKRNDVVIIVNAIVVAVIIGWIDFHATEPQPAAFLLIIFGAI
                     FGFLRPRSAWRWAIILGLSLFVTELIFPLFGLQPIDPPQPNVFATLFALIPAFIGTYA
                     GVVARKITGSLSSDADR"
BASE COUNT         1303 a         1573 c         1616 g         1297 t
ORIGIN      
        1 tgccatgtct tgtggtcgga agacttgagc gaaggacagg attacaacgg aactcaaatc
       61 actaatccct tcactcgcgt tgaagccgat tcctaaaaag ttgttgcttt gtaatctcct
      121 cttctcttac cccagcggcc cgatgttttt cacatactta tcaaagaact tgatcgatcg
      181 actcatcgcc aatccaaaat tgttggcgat gttgtgattg tcgccctcgt aactgtagaa
      241 ctccaccgtc ttgcccgcag cgagcagcgc ttggttgagg ctgactgaaa atgcgtacgg
      301 cacttcttca tcggtcgtgc tgtgatcgag ttggattggg ccggacacat cgctcacgta
      361 atcgatcggc gagatcgaat tccagaaatc gggattctct tccggtgagc cgtattccgt
      421 gatgagatta tttcgccagc gtcgcccctg agaagacgtt tccgacgctg gtgtcggcgt
      481 ttcgcctggt cgcggtcgat gccacttgct caagagatcg gcgtatgacg ccacgacgcc
      541 accccaaatc acacctgctt tgatgtcctg gctgatcacc atcgcgcgca aggtgagtga
      601 gccgcccatc gaatgacccc acatgccgat gcgattcgga tcagcgtcgg gatatttttt
      661 gatcgaggct agcgcgttca gcacatcgat cgtgtaatcg ggcgaaccgt aaccgcccgt
      721 tgcctcgcct tccgaagatc cgtgcccgcg ataatcgggt ttgatcacaa tgtaaccgct
      781 gcgcgcgaac ccggcgacat acgccacgta ccgctcggtc gtgcgataag tttgtggcgg
      841 aatgaagccg tgattgaaaa cgatcaccgg ccaaccggta tcgggtttct ggccaagtgg
      901 caccgtgagc agcgcgtaaa ttttcaaacc ctcggacaaa tacgacgcga tatattgttt
      961 gtagttgctg ccgttcggca attcctgctc gatggtaata tcgctgcccg gatattcttg
     1021 ctggcgcatc acatcgatct gcagtggatt cggcacgggc gtcggcgttg gcgtgatcgt
     1081 cggagtgctg gtcgatgttg gaatcgcggt ggttgtcggc acgatcgacc tcggcgtctg
     1141 agtgaacgct gccgatgtcg gtgccggcgg gttgctgctg catccgatcg agaggatcag
     1201 cacgatcgag atggtgatcg aacgggcgga gaatttgatt ttgttcatgg gtctggctcc
     1261 cttcggtatc ttgagtagga ttgtacccga tcgaagttaa gaattgatga agagcggatg
     1321 aggtctccga aaaaattttc atatcccgtg gcgatagatc ttcttccagc acggaacggc
     1381 tgcccgtttg agcctggaat tgtgttatac tcgctcaggg tgaggacgag atggtccttg
     1441 cccattttgc tacccaaccc gtgaacatga gaacgtaaat ggtaatgaaa gaacaagaaa
     1501 cactctggca cagccgatcg atcgcgcatg tggcctccga gttgggaaca gaaccgaccc
     1561 gcgggctgac ttcggaagaa gcggctcagc gtttgctgcg attaggtccc aatgaactcc
     1621 gcgagaaacc atccacgcca ttctggaaac ttgtgctggc tcagctcaac agttttgtgg
     1681 tgatcttatt gatcgtcgcc agcattatct ccgcgctgtt aggcgattac gtcgaagccg
     1741 cggcgattat ggcaattgtc atcttgaacg cggtgctggg tgtggtgcaa gagagccgcg
     1801 ccgaagcggc attggccgcg ctcaagaaaa tggccgcgcc ggaagccgag gtgatccgcg
     1861 acgggcaccg ccaaaaaatt tccagccgcg aattggtgcc gggtgatgtg gtgctgatcg
     1921 aatccggcaa ctacattccg gccgatctgc gtttagtgga cagcgtcaat ctcaagatcg
     1981 atgagtcggc gttgaccggc gaatcgaacg ccgtcgagaa acgatccgcg gatacgctgg
     2041 ccgagcacgt gccattagcc gatcggcgca acgcggctta tatgagttcg ctggtcactt
     2101 atggccgcgg caaggggatc gtcaccggca ccggaatgga tacacagatc ggcttgatcg
     2161 cgcaaatgct gcaatcattt gaagaagagc cgacgccgct gcagcgcaaa ctcgatcagc
     2221 tgggcaaaac cctcggcacc gcggcggtgt tcttgtgtct gatcgtcttc gtcgtatacg
     2281 ttatccgcgc tactaatttc tccgtgctct ttgcgcccga cggtggcctg ggcgcctact
     2341 tccaagcctt caaagctaaa ctcgccgaag catttttgat cgccgtgagt ttggccattg
     2401 cggccgtgcc ggaaggtctg ccggcggttg tcacgatcag tctggcgctc ggtatgcgcg
     2461 agatgatccg gcgtcacgcg ttgatccgaa aactctccgc ggttgaaacg ctcggctcgg
     2521 ccacgacgat ctgttccgat aagaccggta cgctgacgca aaatgaaatg accgctgtgc
     2581 agttgtgggt gcatcatacc gaagtcttga tcgaggggcg cggctatcaa ccgaacggct
     2641 ctttcctgcg gcccgatcga cgtgacttta atgcgcgcga tgatgtggaa gttgccggct
     2701 tattgtgggc ggcggcttta gccaacgacg ccgaactgga accgacgggc gaagcacagg
     2761 gccagcaaac ctatcgcatg gtcggcgatc ccacggaagg cgcattgatc gttgccgcgg
     2821 caaaagcgga tctctggcgg cgcgagttgg aacaggatta tccgcgtgtg gccgaagtgc
     2881 ccttcgattc ggaacgcaaa cttatgagca ccattcatgg gctgcggcac gttaccgaag
     2941 atgacggcag tccattcttt ccgggagaaa taggctacgt cgtttgcgtt aaaggtgcac
     3001 cgggattgtt gctggaacga tcgacttact tgttacatcg caaaggcccg ctgccgatcg
     3061 aagatcatga tcgagccgcg atcgacgaat cgatctcgcg tatgggctcg caggcgctgc
     3121 gagtgttggg ggtggcttac cgcaaactcg atcggctgcc cagctcaatc gaggctgaag
     3181 agatcgaaca agatttgatc ttcatcggct tgatcgggat gatcgatccg gcgcggcccg
     3241 aagtaaaacc cgcggtgcag aaagcgcgcc aggctggaat tcgaaccgtg atgatcacgg
     3301 gcgatcatcc tgataccgcc cgcgcgatcg ccagcgagat cggtattctg cggcccaccg
     3361 gccaattaca caccggcgtc gagattgaag cgatgagcga cgatcaactg aaggcggttg
     3421 tgccggacac ggatgttttt gcgcgtgtca gtccacagca caaagtccgc attgtcgaag
     3481 cgttccgcgc gcgccatcac gtcgtggcca tgacgggcga tggcgtgaat gatgcgcccg
     3541 cgttgaaacg cgcggacatc ggcgtggcca tgggcatcag cggcaccgat gtcaccaaag
     3601 aaactgcgga tatggtgttg accgacgata actacgtgtc gattgtcggc gcggtcgagc
     3661 aaggccgcat catttacagc aacattcgca agtttgtttt ttatctctta agttgcaatg
     3721 tcgccgaagt cgcgatcatc ttgatcgcag ccatcgccgg ttatccaccg ccgttgacgg
     3781 cgatccaatt attatggctg aatctgttga ccgatggtgc acctgcactg gcactcggta
     3841 tggaaaaagg cgatcccgat attatgcagc agccaccgcg tccatcgcgc gagccgatca
     3901 tcaatcggat catggtcatc cgcatcgcca tcatgacggt ggtcttgacg gcggtggtgt
     3961 tgactgcttt cttgatcggt ctcaacgaac atggcaaaga cttggccgag acgatggctt
     4021 tcgcgacttt ggctttagct gaactcccga tcgctttcac cagccgatcg gagcgctatc
     4081 cggtgtttag gctgggcttc ttctcgaata aatggatgcc gcgcgcgatt gccttgtcgg
     4141 ttgtgttgac gctggcagtc atctatgtgc ccttcctcaa taaacctttc aacaccgtgc
     4201 cgctgacgct tcagcagtgg gaagtcatca tcccgctgat tctgctcccc gcgatcgtcg
     4261 ctgagatcag taaatacttt gtgcgccgca gcgaacatca ctaacttgcg tgaagatcgt
     4321 caatgacaac accaaagtgt gcttgggcga agtaaaagta gtgggttagt tgaatttgat
     4381 tcgataacca cgaagacgct atggcacaca ggtcgtaaaa atcttagcgt cttcgtgcct
     4441 tagtggttta taagatcaaa tttttgtgca gtcctctgtc tactgactcc ccaaatcttc
     4501 cgggtcaaat cgatgatcgg tgcggccatc aacactagcg cgatgatgta cagcggccaa
     4561 ttgaatgacg ttggcagcac atcacgcaca gcttgaagac cttcgctggc tgcgcggagt
     4621 gtgtcggcca taaacgcata caacgcgacg aacacgccgc cgatattcat cagccacgcc
     4681 ccgcggctgg gccagatcgc tcgatcgggt tgatcgggtt gacggaccaa tgtcccgcca
     4741 atgaccatca tcaatgcgat cagcgctggc gcgatcaccg gtccccacca cggcagtgga
     4801 atcaggaaca gaacatccca atcaaagatc gagtgcggcc agccgctgat catggccagg
     4861 aacacgtaat agaaaatgtc ccagatgcca aaagcgatca gcatgtagcc taagcggctg
     4921 cgccgcgatc gaccggcgag ccagccgacg gtgaacagca tgatcaatgt cgccgcttcg
     4981 cgcacgattt cggcttgacc caagccaact gatatgggca atggccgcgc ttgataggga
     5041 tcgattctgc caacgagtgt gcgcaagtaa gtcacagtcg ctgattccat ccaggccatc
     5101 gccagcgaaa aaatgacgac gacaatccag cgtttctttt cagtcattca ttcttctcca
     5161 agcatgcgtg ctgcgtatag cgtcacaagc caatcgattt tacgcattaa gcattccgcg
     5221 cgagatcttt catttgcaat ttcatcgatc agcatcactt gacaaagacc cggtaatttt
     5281 gcgcgccacc acacctgcat acgtcccgat gaaagccggg atcaatgcga atagcgtcgc
     5341 gaaaacgttg ggctgcggcg gatcgatcgg ctgcaggcca aacagcggaa agatcaattc
     5401 ggtcacgaac aaactcaggc ccagaatgat cgcccaacgc cacgccgatc gtggccgcag
     5461 aaatccaaag atcgcgccga agatgatcaa taagaatgcg gcaggctgcg gttcggtcgc
     5521 gtgaaaatcg atccagccga taatgacggc cacgactatt gcattgacaa tgatgacaac
     5581 atcgtttcgt tttgtgttca tagcgcctcc caaaggttac ggacaggaaa tttgattcaa
     5641 gtatacctga tgatcatgca ctgcccacca cgcttgaacg cggctccaat tccatgtgcg
     5701 ccagtcgaca gcctcaggca ctgaccaatc attcaataaa ttaattgcga tctcgcactg
     5761 ggcgaatggc gacatgcttg gcagtgcat
//
LOCUS       DASRTJ010000377        10006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_23431,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000377 DASRTJ010000000
VERSION     DASRTJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23431"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFF70_07590"
     CDS             <1..1098
                     /locus_tag="VFF70_07590"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HZY44595.1"
                     /translation="ADQYPATGLSLRSGTADTIVILDTTEARARSIGQIDRESASHHV
                     HAGAIYIHEGQSFLVEKLDWDNGQAFVKPAQVDYYTEASSAVNVDVENELQREELTTT
                     IKSVGEVSISSLTTGFRKIKLYTHETLGWGDIDLPEQQMHTTAYWFCVEPETADRLIE
                     EGIIFAPNDYGPDWETVRTLILERDGHSCKRCGAVESPGRQHDVHHKKPFREFGYKRG
                     VNRNDQIANQPGNLITLCRACHHRVEAATGLRGALSGLGHVLGEIAPLYLMCDPRDIG
                     VLPESRSAFTELPTVTIYDRVPAGIGLSQELFDLHDQLLAAARDVVASCQCESGCPAC
                     VGPIAPAEAIGDTDLNVKTLTLKLLNALTNR"
     gene            1492..2736
                     /locus_tag="VFF70_07595"
     CDS             1492..2736
                     /locus_tag="VFF70_07595"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZY44596.1"
                     /translation="MKQRRLFLVAIVMLVVVSLACSLGGGDTTQPTAIPQQPQGTPPG
                     QVTLPPIAQVTLPPVVEQPTITPLGPTQAPPPTPTETPPSGTGPGGCVLSAAFVADLT
                     IPDNTIEAPNASFIKTWRIKNNGTCTWDPSYQLVFAEGNQMNGPAGVPINNTLPGANL
                     DVSVNLKAPSLPSGTPYIGKWRLKASNAVIFGGFTVVIIVPLPPTATPTATPTTAPTG
                     AWSGHWETNCGTYGCGVMDLVQTGGTVAGTYAGSSATGTINGTVNGNHLKGTWSISGS
                     NGKIDWWLGSGLVKWRGNWDGVNDWCGHRTGETDPAPCGVGTFDGNWTVVCEGCDGAM
                     HIDQDGKNFTGTYVNGTIDGTIDGDIADGQWHSAGFSGLIKWNLLSYNQFNGHYFGLT
                     PGTFKWCGYRGGATEPVPCDAP"
     gene            2986..3957
                     /locus_tag="VFF70_07600"
     CDS             2986..3957
                     /locus_tag="VFF70_07600"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZY44597.1"
                     /translation="MSLARSLGGTQQQQQQLPPQQQPQGTPIAQNAPTQAPVAAQPTI
                     TPPPPTETPPSGTGPGGCVLNAAFVADLTIPDNTLEAPGASFVKSWRVKNTGTCNWDA
                     SYQLIFAEGNQMSGPASVPINPTASGGTADLSVNLTAPAAPSSPNYIGKWRLKASNGV
                     IFGGLTVVIVVPAPPTSAPTAAPTAVPPTAAPPGPGVTLVAIPPGGIIVSCVLFGTCP
                     GQKIDHYVGSWSGGTCGGFMSADISKSGSTLNISVSGLFGGGLISALTGNVPFGGEPV
                     HYDYNYSIGFPLNLDYTFHVVLDMPDNVNTLHVVTTGDASLDCNLIK"
     gene            4036..4554
                     /locus_tag="VFF70_07605"
     CDS             4036..4554
                     /locus_tag="VFF70_07605"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZY44598.1"
                     /translation="MLTRLIFPAQRWLLILIIVSAALLSACMDRPTKTDPAGSMAAAI
                     VAATHQVTVTPISTAFVPCDNDAKFVADINLLDGTHLMPGTLFTKTWRVRNIGTCSWT
                     TDYQLKFIGGSSLRESIVTLAQIVKPNDTVDISIALSVPATVGSYRGRWRLFAADGTP
                     FGQTLFVEIAVP"
     gene            complement(4728..6167)
                     /locus_tag="VFF70_07610"
     CDS             complement(4728..6167)
                     /locus_tag="VFF70_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HZY44599.1"
                     /translation="MTGFIVPVYTSAMLPFRSQLLRVTFMAICSIAIGLSIQFALAQS
                     LSPSQDDASAFRRPERALEGGAISFTPTYTIDLPLILQNYPVMHEVRALWISRFDWTT
                     SHHTVTTTDIDTIVNNVASAHFNMILFQIRGTADAFYSSTLEPWASRLTGEVTRTLGQ
                     SPGFDPLAVMIDRAHAQGIQVHAYMNVYPTWLCDSIGPITNTTPQHPFWQWTAQSPST
                     YGSKTFFWREWSNTSLQPMNLIPTSQGGTCGSPDYLGGYLWASPAVTIVQSHILSVAI
                     DLATRYDIDGLHLDNVRYSGSNYSSDPTTHQAFTAVHTLSPTLVYTTWLPNFQRAQVS
                     NLVVQIYSATTAEKPNLWISAAVWPNYTSGTNTYFQDSKGWLANGSVDANMPMLYSSD
                     VINDLSAWTNRAQDFVNDAHGRYIIPGISGDYTNFNDIVDRINAARSIGAAGVAVFSY
                     GALNARNYWDDLANGPFATPAIIPQPNWK"
     gene            6244..8664
                     /locus_tag="VFF70_07615"
     CDS             6244..8664
                     /locus_tag="VFF70_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZY44600.1"
                     /translation="MTKQTTKSKKNGSKSRKKQSSQPLIQLSLDQKLDIFGVVLVGTG
                     VVTVLSMLSKTQGSVTGEWIKGLYLAFGLGVYVVPIAFVLVGLWLLLRSFERTPRLSG
                     EQIVGVIVFFILALITLAEFDPIMGGSLGAGLLKLIDGGIGWIGTIVLVVVGWLISIV
                     LLFNVAPSEIAAWFARLLRPLRGSRPSASPIGDARPSAMAININTRPQNVAASSGNGN
                     GKKTAASFEPDFQHLASSTAQSAAATSDRISNLPIVQPRLRPDAPPPGMLGDQTMPQP
                     FSGLSSEASMPDQPATGPLYAHIIGGEQAWILPSIAEIFEGGEEASMSEDDLRGKARI
                     IEDTLRSFGVEGKVIEVNRGPTVTQFGVEPGFVQRGGKETKVKVSKISALADDLALAL
                     AAKTIRIEAPVPGKGIVGIEVPNNEVSTVALRDVIESEAFQKLKSHLRIALGQDVSGQ
                     AISADLANMPHLLIAGTTGSGKSVCVNAIICCLMAYNTPDELKFLMVDPKRVELTTYN
                     GVPHLLAPVIVDLERVVGVLQWVTREMDERYRKFAKSGSRNIDDYNTKAIDTSTEKLP
                     YIVVVIDELADLMMLAPDETEKTICRLAQMARATGIHLIIATQRPSVDVVTGLIKANF
                     PARIAFSVASSVDSRVILDTTGAERLLGRGDMLYVAPEIGAPQRLQGCFVGDREIHAL
                     VRYWKGIRGAVVEGAATIPSFANQPQQPMQATQPPLWESMRAEIDAANANGEDDLLQR
                     AIDVVKMQRKASISLLQRQLRIGYTRAARLIDLMEEKGIVGPQAEGNRWREVMFDSPL
                     LGSLSDED"
     gene            8946..10004
                     /locus_tag="VFF70_07620"
     CDS             8946..10004
                     /locus_tag="VFF70_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY44601.1"
                     /translation="MRKVTLISIVMLVVAGMLLSACAPAATPAAAKKKIAAVLDTGGE
                     NDKSFNQYTLQGAKDAAAKLGFDMSYVVSASSSDYEKNIETFVSEKADMIITVGFLMG
                     DATAAMAKKYPNIKFAIIDVAYFPTAGSAACPDTAKDCYADGGLTNVTSLMFQEDQVG
                     YEAGVLAGCMSKSGTVATVSGMEIPPVVRYVVGFQNGAKSVNPNVKTLNIYIPNFNDP
                     TTGKQAGADFVSQGADVVFGVGGNTGNGGLLAAKEKGLMAIGVDVDQYNTYPEVKDAL
                     ITSAQKNMDVATNAAVTAFAAGTLKAGIQTGNAANGGIGLAPYHDWDSKISADCKAKV
                     DAAVKGLKDGSVQTGYKP"
BASE COUNT         2273 a         2643 c         2838 g         2252 t
ORIGIN      
        1 gcggatcaat atccggcgac cggcctatcg ctccgcagcg gcacggcgga tacgatcgtg
       61 atcttggaca ccaccgaggc ccgcgctcga tcgatcggcc aaatcgatcg agagtcggcc
      121 tcgcatcacg ttcacgctgg cgcgatctac atccacgaag gtcaatcgtt cttagttgaa
      181 aaactcgatt gggacaacgg ccaagcattt gtgaagcccg cgcaagtcga ttactacacc
      241 gaagcgagca gcgcggtgaa tgtcgatgtt gaaaatgaat tgcagcgcga agagttaaca
      301 acaacgatca agagtgtggg cgaagtcagc atctcctcac tgacgaccgg ctttcgcaag
      361 atcaaacttt acacgcatga aactttgggt tggggcgaca tcgatctgcc ggaacagcaa
      421 atgcacacca cggcttattg gttttgcgtc gaacctgaaa cagcggatcg attgatcgaa
      481 gaaggcatca tctttgcgcc gaatgattac ggcccagatt gggaaacagt tcgaacgttg
      541 atcctcgaac gggatggaca tagctgcaaa cgttgcggcg cggtggaatc cccaggccgg
      601 cagcatgatg tgcatcacaa aaaaccattt cgcgaatttg gttataagcg cggcgtgaat
      661 cgaaacgatc agatcgcgaa tcaacccggg aatttgatca cgttgtgccg cgcgtgccat
      721 catcgcgtcg aagcggccac aggtttgcgc ggcgcgttga gcggcctcgg tcatgtgctg
      781 ggcgagatcg cgccgctgta tttgatgtgc gatccgcgcg acatcggcgt gctgcctgaa
      841 tctcgatcgg ctttcacaga attgccgacc gtcacgatct acgatcgagt tccggcgggg
      901 attggtctta gccaggaact atttgacttg cacgatcaat tgctcgcggc ggcgcgcgat
      961 gtggtagcca gttgccaatg cgagtccggt tgcccggcct gcgtcggacc gatcgcgcca
     1021 gctgaagcga tcggggatac cgatctgaac gtgaaaacgt tgacactcaa attgttgaat
     1081 gcattgacga atcgataaga aattgggccg catataaatc gatcaacgag agagataacg
     1141 gccagcgccc atcacagcgg ggggttgtga cgggcgcggc agccaaggga ggagactcag
     1201 ttttgggtgg ctggtaaaag ccacgttcaa ctcagtgcgg agctagaata tcattgttgc
     1261 cagttatgct ctagtgtcat gtatcatgtt acagagatga cgcttgtcgc gctggtacac
     1321 acttaccgaa gaataaaggg atagttatct tttatattcg agtaaaggta gtagccggac
     1381 cgtacttgat ttgatttatc ggctataatt gggcatcaat gttaggcggg cgggtgcttg
     1441 gccagcccta tggtgcagca cccatcattc catcagggga ggagaaaaaa aatgaaacag
     1501 cgccgtttat tcttagtcgc cattgtaatg ctagttgtag tcagcctggc atgttcgttg
     1561 ggcggaggtg acacgacaca accgaccgca attccgcagc aaccgcaagg cacgccgccg
     1621 ggacaagtta ctctgccgcc gattgcgcag gtgacgctgc cgccagtcgt ggagcaacct
     1681 acgattacac cgctcggccc gacgcaagcg ccgccaccta cgccaacgga gacgccacct
     1741 agcggcacgg ggccgggcgg ctgtgtgttg agtgcagcct tcgtcgcaga cttaacgatt
     1801 cccgataaca ctattgaagc gcccaatgct tccttcatta agacgtggcg tatcaagaat
     1861 aacgggacct gcacctggga tcccagttat caattggtgt tcgccgaagg caatcagatg
     1921 aatggcccgg ccggcgtacc aatcaacaac acgctgcccg gcgcgaatct ggatgtgtcc
     1981 gtaaatctca aagcgccaag tttgcccagc ggcacgccct acattggcaa gtggcgcttg
     2041 aaggcgagca acgctgtgat cttcggcggc ttcaccgtag tgatcatcgt accgctaccg
     2101 cctacggcca cgccaaccgc tacacccacg accgctccta ccggcgcatg gtctggacat
     2161 tgggaaacaa attgcggaac gtatggctgt ggcgtaatgg atctggtgca gacgggcggc
     2221 actgtcgctg gaacatatgc cgggagcagt gccaccggca cgattaacgg taccgtcaat
     2281 ggaaatcatc tgaaaggcac gtggtctatt tcagggtcca acggtaagat tgattggtgg
     2341 cttggctcgg gactggtcaa gtggcgtggc aactgggatg gcgtgaacga ttggtgcgga
     2401 catcgcaccg gggaaaccga tcccgcgccg tgcggcgtcg gcacgttcga tggtaattgg
     2461 accgtcgtct gtgagggttg cgatggcgcc atgcatatcg atcaagacgg caagaatttc
     2521 actggcacct atgtgaatgg caccatcgac ggcacgatcg atggcgacat cgctgacggc
     2581 cagtggcaca gtgccggttt ttccggcctt attaaatgga atttgctgag ctacaatcag
     2641 tttaatggtc actattttgg gttaactccg ggaaccttta agtggtgcgg ttatcgtggt
     2701 ggcgcgaccg aacccgtacc ttgcgatgca ccgtaatcgc actgtaaagt aatgatgctg
     2761 tttagattga acagggttga gtttcgttct cagccctgtt cctattttta tactcgattt
     2821 cttaacgcag ctgttagcaa cttgatagtt caacttgctg cgttttggat tttaatgtcc
     2881 gcaatttggg aacacgccaa gtgtcattcg atcaactaca ggagggagag cattaaacat
     2941 caacagcgtt aaccattctt agctgtgatt gtcgttttga tcgtggtgag cttagctcgt
     3001 tcactcggcg gaacgcaaca gcaacaacag caactgccgc cgcagcaaca accgcaaggc
     3061 acgcccattg cgcagaacgc gcctactcaa gcgccagttg ccgcccagcc caccatcacg
     3121 ccgccaccgc cgaccgaaac tccgccgagc ggcactggac ccggcggttg tgtcttgaat
     3181 gccgccttcg tcgccgatct gacgatcccg gataacaccc ttgaagcgcc cggcgcgtcc
     3241 ttcgttaagt cgtggcgagt gaaaaatact ggcacctgta attgggatgc cagttatcaa
     3301 ttaatcttcg ccgaaggaaa tcaaatgagc ggcccggcga gtgtgccgat caatcctacc
     3361 gcgtccggtg gcacggctga cctctcagtt aatctgactg cacccgccgc gcccagctcg
     3421 cccaactata ttggcaagtg gcgcttgaaa gccagcaacg gcgtgatctt tggcggactg
     3481 accgttgtca ttgtcgtgcc tgcaccgccg acctctgccc caactgccgc accgactgct
     3541 gtgccaccaa ccgctgcgcc gcccggtcca ggtgttacgc tggtcgctat accgccgggc
     3601 gggattatcg tttcttgcgt cctctttggc acctgtcccg gtcagaagat cgatcactat
     3661 gtaggctcgt ggtccggcgg aacgtgcggc ggcttcatgt cggcggacat ctcaaagtcc
     3721 ggctcgactt tgaacatctc cgtgagcggc ctcttcggcg gcggattgat atccgcgtta
     3781 accggcaatg tgccgttcgg cggtgagcca gtacactatg attacaatta cagcatcggc
     3841 tttccattga atctcgatta caccttccat gtcgttttgg atatgcccga caacgtcaac
     3901 acgctgcatg tagtgaccac tggcgatgcc agtctcgatt gtaatctgat caaatagatc
     3961 gattcgatcc tcttccgaac gagccgcctc agctgctgag gcggctcgtt gcgttgatca
     4021 gctataataa gccgtatgct aacccgactg atcttcccgg cccaacgctg gctgctcata
     4081 ttgatcatcg tcagcgcggc cctgctatca gcttgcatgg atcgaccgac aaaaacagat
     4141 cccgctggct caatggccgc tgcaatcgtg gcagccactc atcaagtcac tgttacacca
     4201 atcagtacgg cctttgtgcc ttgcgacaat gatgctaaat ttgtcgccga catcaatcta
     4261 ctggacggca cgcacctcat gcccggcaca ctcttcacca aaacttggcg agttcgaaat
     4321 attggcacgt gcagttggac gaccgattat caactgaagt ttattggtgg atcatcgctg
     4381 cgcgaatcga tcgtcacact ggcgcagatc gtcaaaccca atgacaccgt cgatatttcg
     4441 atcgcgctca gcgtgccggc gacggtcggc agttatcgcg ggcgctggcg tttgttcgcc
     4501 gccgatggaa cgccgtttgg acaaactctg tttgttgaaa tcgcagtgcc ttagagcggt
     4561 tccaaaatga ttgatggcgt cacacccgcg cccttcggtc gtaaaacccc acagggagta
     4621 tcaaatgcgg gtgtccagtt tttttacact cgattcccgc atacgcggga atgacagtgt
     4681 catattccaa attggaattt ggccgtagtc aatgttttat cgatttgtta tttccaattc
     4741 ggctgaggaa tgatcgcggg tgttgcgaag gggccgttcg ccagatcgtc ccagtagttg
     4801 cgggcattga gcgcgccgta tgaaaaaaca gcgacacctg ccgcgccgat cgatcgtgca
     4861 gcgttgatgc gatcgacaat gtcattgaag ttagtgtagt ctccgctgat gcccggaatg
     4921 atgtagcggc catgcgcatc attcacgaag tcctgcgccc gattagtcca cgctgagaga
     4981 tcgttgatca catcgctcga atataacatc ggcatgttcg catcgacgct gccatttgcc
     5041 agccagcctt ttgagtcttg gaaataagtg ttggtgccgc tggtgtaatt cggccagacg
     5101 gcagcgctga tccacaaatt cggtttttcg gcggtcgtcg ccgaatagat ttgtaccaca
     5161 agattgctga cttgcgcgcg ttgaaagttc ggcagccagg ttgtataaac caacgttggc
     5221 gatagtgtgt gcacagctgt aaaggcttga tgcgttgttg gatcggatga gtagttcgat
     5281 ccggagtagc gcacgttatc aagatgcagt ccgtcgatgt catagcgcgt ggcaagatcg
     5341 atggcgaccg acaggatgtg cgattggacg atcgttaccg ccggactggc ccacaaatat
     5401 ccgccgagat aatcgggact gccgcacgta ccgccttggc tagtcggaat caaattcata
     5461 ggttgcagtg aagtgttcga ccattcgcgc cagaaaaatg tttttgagcc gtaggtggat
     5521 ggcgattgcg ccgtccattg ccaaaacgga tgctgcggcg tggtgttcgt gatcggcccg
     5581 attgaatcac acaaccacgt tggataaaca ttcatgtagg cgtgaacttg aatgccctga
     5641 gcatgcgcgc gatcgatcat gaccgccagc ggatcgaagc ccggcgattg ccccaacgtt
     5701 ctggttacct cgccggtcaa gcgtgaagcc cacggttcta acgtcgatga ataaaacgca
     5761 tcggcagtac cacgaatttg aaataggatc atattgaagt gtgccgacgc gacattgttc
     5821 acaattgtat cgatgtccgt cgtggtgacg gtgtgatgag atgtcgtcca atcgaagcgg
     5881 ctgatccaca acgcgcgcac ttcgtgcatc accggataat tttgtagaat caacggtaga
     5941 tcgatcgtat aagtgggcgt gaatgaaatt gccccaccct ctaacgccct ctctggacga
     6001 cgaaatgcag aggcgtcatc ctgggaggga gaaagtgatt gagccaaagc aaattggatg
     6061 ctcagcccaa ttgcaatcga gcagatcgcc atgaatgtta cgcgcagcag ttgagatcga
     6121 aatggaagca tggctgaagt atatactgga acaataaagc ccgtcaaaaa attccttata
     6181 gatttttgta agggcttatg ctataatctc catcgcatcc gaacattcgt cctaaccgga
     6241 actatgacca agcaaacgac caaaagcaaa aaaaacggca gtaaaagccg caaaaaacaa
     6301 tcaagccagc cgctgattca actctcgctc gatcagaagt tggatatctt cggggtggtg
     6361 ttggtgggca ccggcgtcgt caccgtcctc agcatgctct ccaagacgca aggcagtgtg
     6421 actggtgaat ggatcaaggg actgtatctc gcgttcgggc tcggtgtgta tgtcgtgccg
     6481 atcgccttcg tgctggtcgg cctgtggttg ctgctgcgat cgttcgagcg cacgccgcgc
     6541 ttgtccggcg aacagatcgt cggcgtaatc gtgttcttca ttctcgcctt gatcactttg
     6601 gctgaattcg atccgatcat gggcggctcg ctgggtgccg gtttattgaa attgatcgat
     6661 ggcgggatcg gttggatcgg cacgatcgtt ttagtggtag ttggttggtt gatctcgatc
     6721 gtgctgctgt tcaatgtcgc gccgagcgaa atcgccgcgt ggtttgctcg attacttcga
     6781 ccgctgcgcg gctcgcggcc atctgcttcc ccgatcggcg atgcacgccc atcggccatg
     6841 gcgatcaaca tcaacacgcg tccgcaaaat gtcgccgcgt cgagcggcaa cggcaatggc
     6901 aaaaaaaccg cggcttcatt cgaacccgat tttcaacacc tggcgtcgag cacagctcaa
     6961 tcggctgcag ctacgtccga tcggatctcc aatctgccga tcgttcagcc gcgccttcga
     7021 ccggatgcgc cgccgcccgg catgctcggc gatcaaacga tgccgcagcc tttttcagga
     7081 ttgagctcag aggcgtcgat gccggatcag cctgccactg gcccgttgta tgcgcacatc
     7141 attggtggtg agcaggcgtg gatattgccc agcatcgctg aaatatttga aggcggtgag
     7201 gaagcatcca tgagtgaaga tgatctgcgc ggcaaagcgc gtatcatcga agacacgctg
     7261 cgatcatttg gtgtggaagg caaagtcatt gaagttaacc gcggcccaac tgtcactcag
     7321 ttcggggttg agccgggttt tgtgcagcgc ggcggcaaag aaacgaaagt gaaagtcagc
     7381 aagatcagcg cgctggcgga cgatctggct ttagctttag ccgccaaaac aatccgcatt
     7441 gaagcgcctg taccgggtaa aggcatcgtt ggcattgaag tgcccaacaa cgaagtctcg
     7501 acggtggcgc tgcgcgatgt gatcgaaagc gaagcctttc aaaaactcaa atcacatcta
     7561 cggattgcgt tgggtcagga tgtgtccggt caagccatct ccgccgatct ggcgaacatg
     7621 ccgcacttgt tgattgcggg caccaccggt tcaggcaaga gtgtgtgcgt gaatgcgatc
     7681 atctgttgtt tgatggccta caacacgccc gatgaattga aattcctgat ggtcgatccg
     7741 aagcgcgtgg agttgacgac gtacaatggc gtaccgcatt tgctggcgcc ggtcatcgtc
     7801 gatctggagc gcgtggtcgg cgtgctgcaa tgggtcacgc gtgagatgga tgagcgttat
     7861 cgcaagtttg ccaagagcgg ttcgcgcaat atcgacgatt acaatacgaa ggcgatcgac
     7921 acgagcaccg agaagctgcc gtacatcgtc gtcgtgatcg atgagttagc cgacttaatg
     7981 atgctcgcgc cggatgaaac ggaaaaaacg atctgccgtt tggcgcagat ggcgcgcgcg
     8041 accggcatcc acttgatcat cgcgacgcag cgacccagcg tcgatgtggt caccggcttg
     8101 atcaaagcca acttcccggc tcgcattgct ttctcggttg cgtcgtcggt cgattcgcgc
     8161 gtgatcttgg atacgacggg cgctgagcgg ttgctcggtc gcggcgatat gctgtatgtt
     8221 gcgccggaga tcggcgcgcc acagcgcttg caaggctgct tcgtcggcga tcgtgagatc
     8281 catgcgctcg tgcgttattg gaaaggcatc cgcggcgcag tcgtggaagg cgcggccacc
     8341 attccgagtt ttgccaatca gccgcaacaa ccgatgcagg cgacgcagcc gccattgtgg
     8401 gaatcgatgc gcgccgagat cgatgcggcc aatgccaacg gcgaagatga tctactccag
     8461 cgcgcgatcg atgtagtgaa gatgcagcgc aaggccagca tctctttatt gcagaggcag
     8521 ctgcgtatcg gctatacgcg cgcagcgcgg ctgatcgatc tgatggaaga gaaaggcatt
     8581 gtcgggccac aggccgaagg caaccgttgg cgcgaagtga tgtttgattc gccgttgctc
     8641 ggttcgttaa gcgacgaaga ttgatcgatc gcgctgagct cgaccgatcg gttggtgaaa
     8701 atacccgcga atcttcttca tcgatcgaat ttgccagacg gatcgatctg cactataata
     8761 gcctctagat tgtttactcc gcaccacttt gcctgagtat gaggtccatc cttctggact
     8821 tattagctgt gcaatcatca gttctcgaaa agggggtgtt gatctccagt taaattttag
     8881 tcggatattt ttccccaacg taatgcgttt ttcaacaact caattctaac aaggagaaga
     8941 atagaatgcg taaagtaact ttgatttcga tagtaatgtt agtggtcgcc ggcatgctgt
     9001 tgtcggcctg tgcgccggcc gccactccgg ccgcagcgaa aaagaagatc gccgcggtgc
     9061 tggacaccgg cggcgagaac gacaagtcct tcaatcagta caccctccaa ggtgcgaagg
     9121 atgcagccgc caagctcggc tttgacatgt cgtatgtcgt gtcagcctca tcgagcgatt
     9181 acgagaagaa cattgagacg ttcgtgtctg agaaagctga catgatcatc accgtcggtt
     9241 tcttgatggg tgatgcgact gcggcaatgg cgaagaagta tccgaatatc aagttcgcga
     9301 tcatcgatgt tgcttacttc ccgacagcag gatccgcagc atgccctgac acggcaaagg
     9361 actgctatgc cgatggtggc ctcaccaatg tgaccagctt gatgttccag gaagatcagg
     9421 tcggttatga agccggtgtg ttagccggtt gcatgagtaa gagcggcacc gttgccaccg
     9481 tatctggcat ggaaattccg ccggtggtgc gctatgtcgt cggcttccag aatggcgcca
     9541 agtcggtgaa tccgaatgtc aagacactca acatctacat ccccaacttc aatgatccaa
     9601 ccaccggcaa gcaggccggt gcggacttcg tcagtcaggg tgcggatgtg gtcttcggcg
     9661 tcggcggcaa caccggcaac ggtggtctgc tggcggcgaa agagaagggc ttgatggcga
     9721 tcggtgttga cgtcgatcag tacaacactt atccggaagt caaggatgcc ttgatcacca
     9781 gcgcgcagaa gaacatggac gtggcgacga atgccgccgt gaccgccttc gcggctggca
     9841 cattgaaggc tggcattcag acgggcaacg cggccaatgg tggtatcggt ttggccccat
     9901 atcacgattg ggatagcaag atctcggctg actgcaaggc taaggttgat gcggctgtta
     9961 agggcctgaa agacggctcg gttcagaccg gctacaagcc ttaaag
//
LOCUS       DASRTJ010000378         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_326305,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000378 DASRTJ010000000
VERSION     DASRTJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326305"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2096)
                     /locus_tag="VFF70_07625"
     CDS             complement(72..2096)
                     /locus_tag="VFF70_07625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44602.1"
                     /translation="MRRYLPILIILLIAAALRLIALDQVPPGLQHDEVIHGHDAITVL
                     LGYHPLYFTSNAGNEPLFIYLMSATINLLGTNLFGIRIAAVMCGLLTILFSYLWIRRA
                     FNHRTALIAASFIAVNFWPLFLSRVGLRAASLPMMVALTAYLFSPILSQKDDGKSLGR
                     SSIMAGIALGLTLYTYPASRVFPIVFFLFWLLHSVFSRRLHKSALFILIAALITMAPL
                     AYTIATLPQGDQRLQQLGGPVEDALHGYLQPVLTYTIQTLGMFTFAGDPITRYNIPGR
                     PVFDPITGLLFYFGLLLTIKHRRDPRNLFALIWLPIGLLPSMLSDSAPSFLRASVSLP
                     VTFLFPSIAMDWGIQEAFVRTGKGWHRKYQSTFAVLYKLLPIAAILVIGLNCVLTIHA
                     YFVVWPNQSDVREVYRSDLAQAARWIEQQPTRTAPLSGASADQPIVIASTNPRDLDPF
                     LYDFELNGQHDLRWIDRAYALAFPAQQSILISPAYAPIDPILRSRFLPEPSFVSKFGD
                     GSTAFEVYDLDWASTRLAPTNIITTARTDSLTLTVPINIGHSLQFLGYETQSIAQPGD
                     MLKLTLYWRVSKDVGAQQLPLSLFVHVLNASGDFAAGRDLLAYPTAGWRDDDVWLQQN
                     VLQLPSPLDAGTYRVEIGAYSQADNVRWRIYDAAGNDVGARLLLSEVVIR"
     gene            complement(2134..3546)
                     /locus_tag="VFF70_07630"
     CDS             complement(2134..3546)
                     /locus_tag="VFF70_07630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44603.1"
                     /translation="MSRSTAVLLLLATLVAYLTIGTAYALNTPDWQNPDEPAHYNYIK
                     FIVEHQALPVLQSGDYDETYNDQFTRTPQNTHSMSIDPFRYEFYSPPLYYLLAAPIFA
                     VTDGWLSALRLFSVVLGGTLVVFAYRIGAELFPDRLQIAVGAAALVAFVPQHVAMMSA
                     VNNDSLAEPLIALCIWQSIRLFKTDQPSSRSLFLLGGTLGFGLLTKQTVYYTALPIVV
                     VALFLHARKIQATSDQRPARDRWSFVTSSPKRMRSGIRHLSFVFIPAIVLGGILWLHN
                     LYVYGGLDVLGLARHNSIVVGQATTAAWIDQFGLGGLLSRGFTTTFHSFWGQFGWMAV
                     PMPDSTYLLLGLLSGVALLGWLWWLIDRRKMQVASSKLQGAILLLLLFVLTFGGFIYY
                     NLTFVQHQGRYLFPALIPIGLVVAIGLDHILQKIFGLIKNPNEWMAMAQMLIFAAVFI
                     LLARLDIIALQRYIVPFLSP"
     gene            complement(3670..>3775)
                     /locus_tag="VFF70_07635"
     CDS             complement(3670..>3775)
                     /locus_tag="VFF70_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZY44604.1"
                     /translation="MSNGPFKKIEDLKNVPRIGDALFEKIKGLITVGP"
BASE COUNT         1017 a          983 c         1029 g          746 t
ORIGIN      
        1 ttgcccgctt gatcaagcgc aatccatctg catagggcga gccaatcatt gggcaaccac
       61 gaggtttgcc tctaccgtat tacaacctct gataacaaca atcgagcgcc gacgtcattc
      121 cccgccgcat cataaattcg ccagcgcaca ttatccgcct gtgaatacgc gccgatctcc
      181 acacgatacg tgcccgcgtc caatggtgac ggaagctgca aaacattctg ctgaagccac
      241 acatcgtcgt cgcgccagcc cgctgttgga tacgccagca gatcgcgccc ggctgcaaaa
      301 tctccgcttg cattcaatac gtgaacaaat aaagacagcg gcagttgctg cgcccccacg
      361 tctttactca cccgccagta caacgtcaac ttcaacatat cacctggctg cgcgatcgat
      421 tgagtttcat aacctaaaaa ttgcagcgaa tggccgatat tgatcggcac cgtcaaggtg
      481 agcgagtctg ttctagcagt cgtgatgata tttgtagggg caagccttgt gctcgcccaa
      541 tcaagatcat aaacttcaaa ggccgttgag ccgtcaccaa acttcgagac gaaagaaggt
      601 tcgggcaaga aacgcgatcg taagatcgga tcgatcggcg cgtaagcggg cgagatcaag
      661 atcgattgct gtgccggaaa ggccagcgca taagcccgat cgatccagcg cagatcatgc
      721 tggccgttta actcgaaatc atacaggaac ggatcgagat cgcgcggatt ggtggacgcg
      781 atcacgatcg gttgatcggc acttgcccca ctgagtggcg cagtgcgggt cggctgctgt
      841 tcgatccaac gagcggcttg cgctaaatcc gatcgataga cttcccgcac atccgattga
      901 ttcggccaca cgacaaagta agcgtgaatc gtcaaaacac aattcagccc gatcaccaaa
      961 atcgcggcga tgggcaatag cttatacagc acagcaaagg tcgattgata tttgcgatgc
     1021 caacctttgc ctgtgcggac gaaagcttcc tgaatccccc aatccatcgc gatggacgga
     1081 aacaaaaagg tgacgggcag tgacacgctc gcgcgcaaga acgacggcgc ggaatcgctg
     1141 agcattgagg gcagcaaacc gatcgggagc cagatcaacg caaaaagatt gcgcggatcg
     1201 cgccggtgtt tgatcgttag taacaaaccg aaataaaaca acaatcccgt gatcggatca
     1261 aagaccggac ggcctggaat gttatagcgc gtgatcggat cacccgcgaa ggtgaacatg
     1321 cctaaagtct ggatagtgta agtcaacacc ggctgtaagt agccgtgcaa tgcatcttcc
     1381 accggcccgc ccaattgctg caagcgttga tcaccttgcg gcagtgtggc gatagtgtaa
     1441 gcgagcggcg ccatcgtgat gagtgccgcg atcaagataa agagagcgga cttatgaagc
     1501 cttctggaga atacagaatg cagaagccag aagagaaaga agacgatagg gaacactcgt
     1561 gatgctggat aagtgtagag cgttaggcct aatgcaatgc ccgccataat cgatgaacgc
     1621 ccaagtgatt tcccgtcatc tttttgactg agaattggcg agaagagata ggcggtcaac
     1681 gcgaccatca tcggcagcga ggcggcgcgc aaacctactc gactcaagaa caacggccaa
     1741 aaatttacgg cgatgaatga cgcggcgatc aaggctgtgc ggtgattgaa agcccggcgg
     1801 atccacaagt agctgaagag aatggtgagc agtccgcaca tcacggcggc gatccgaatg
     1861 ccaaacaggt ttgtgcccag cagattgatc gtcgccgaca tgagatagat gaacagcggc
     1921 tcattgcccg cgttcgaagt aaagtacagc gggtgataac cgagcaggac ggtgatcgca
     1981 tcgtgaccgt gaatcacttc atcatgctgc aaaccgggcg gcacttgatc gagcgcgatc
     2041 aagcgcagcg cagcggcgat gagaagaatg atcagaattg gcagataacg tcgcatgggg
     2101 aattttcggg taggggtgat cagccgattg cccttatggt gataagaagg gaacgatata
     2161 ccgttgcagg gcgatgatat ccaaccgcgc gagcaggatg aacactgcgg cgaagatcaa
     2221 catctgtgcc atcgccatcc actcattagg atttttgatc agcccgaaaa tcttttgcag
     2281 tatatgatcc aacccgatcg cgaccaccaa cccgatcgga atcagcgccg gaaataaata
     2341 tcgaccttgg tgctggacga aggtcaagtt gtagtaaatg aatccgccaa aggtcaatac
     2401 aaacagaagc aataaaagga ttgccccttg caacttgcta cttgcaactt gcatcttgcg
     2461 cctatcaata agccaccata gccaaccaag caacgccacg cccgacaaca agccgagcag
     2521 caaatacgtg ctgtcgggca tcggcacggc catccagccg aattgacccc aaaacgaatg
     2581 gaaagttgtc gtgaatccgc gactgagcaa tccgcccaat ccgaactgat cgatccatgc
     2641 ggcagttgtt gcctgtccca ccacgatcga attgtgacgc gccaaaccca gcacatcgag
     2701 tccgccgtag acgtagaggt tgtgaagcca tagtattcca ccaagaacaa tggccggaat
     2761 aaaaacgaat gacaaatgac gaatcccact ccgcattcgc ttcggggacg atgtgacaaa
     2821 tgaccaacga tctcgtgccg gtcgctggtc gctggttgct tgaatctttc ttgcgtgaag
     2881 gaacaacgca acgacaacaa tcggcaatgc cgtgtaatag accgtttgct tcgtcagcag
     2941 tccaaagccc agggtccccc ctaaaagaaa taatgatctt gatgaaggtt gatccgtttt
     3001 gaacagccga atcgattgcc agatgcacaa cgcgatcaac ggctcggcca gcgaatcatt
     3061 gttgacggca gacatcatgg cgacatgctg cggcacaaac gcgacgaggg cggcagcgcc
     3121 gacggcgatc tgcaatcgat cggggaagag ttctgcgccg atccgatagg cgaatacgac
     3181 cagtgtgccg ccaagaacga ccgagaacag ccgcaatgcg gaaagccagc catcggtcac
     3241 agcaaagatc ggcgcggcga gcagatagta caacggcggc gaatagaact cgtagcggaa
     3301 cggatcgatc gacatcgagt gcgtgttttg cggcgtgcgc gtgaattggt cgttataggt
     3361 ttcgtcgtag tcgccgcttt gcagaacggg cagggcctga tgctcgacga tgaatttaat
     3421 gtagttgtag tgcgcgggct cgtccggatt ttgccaatcg ggggtattca gcgcataagc
     3481 cgtgccgatc gtcaggtagg cgacgagcgt ggcgagcagt agcaatactg cagttgatcg
     3541 ggacatgctc gctattgtac cagatgggaa aagtgacgag tgatgtgtga tgcgtgaaag
     3601 gcaataaatg gtaatttgtg actgctgcgc tgtcctgcca tggaagtgta tgcaagctgc
     3661 tatcggtcat tacggtccaa cggtgatcaa ccccttgatc ttctcgaata gcgcgtcgcc
     3721 aatgcgcggt acgttcttga ggtcttcgat ctttttgaat ggaccattac tcatc
//
LOCUS       DASRTJ010000379         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_199139,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000379 DASRTJ010000000
VERSION     DASRTJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199139"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFF70_07640"
     CDS             <1..251
                     /locus_tag="VFF70_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44605.1"
                     /translation="QSLRMQEYRYGVLSLISRFFGLFGLHKPVRYARPVEAGTYDTAL
                     HTDHPDAPSFLDQYLDQPEPHLGGAPNVTAKPDETEKL"
     gene            318..>3017
                     /locus_tag="VFF70_07645"
     CDS             318..>3017
                     /locus_tag="VFF70_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZY44606.1"
                     /translation="MNLEPLHALRPTDVFKALETFAQGLTTAEAESRLTLYGRNALAE
                     PPAVPQARKFIGHITHPMGLLLLIASGPAFISGHVVLGFIILIVVIFNAVFSYWQEYR
                     AERAIHALKQLLPVYARVIRDGAETKVPADTVVPGDVLVLAEGDNIPADARVVEEYGL
                     RLNNSTLTGEALPARKTADAALRRDVTEVEQPNLVFAGTSVVSGTGRAVVFASGMTTQ
                     FGRIANLTQTVEQEPSLIQQQMKRITRMITYFALGLGIIVLFVSTADLNLPALDAIIL
                     AVGIIVAVVPEGLLPTVTLSLAIAVRRLAQRGVLVKQLSVMETLGKTSVICTDKSGTL
                     TQNQMTVREVWASDQRLTVSGVGYEPKGEFSAIVSSDLDALLISAALCNNARLTSPTI
                     DHPHWTALGDQTEAALRVMALKGGLDESAINSQFPRVHELPFDARRKRMTTIHRTARG
                     EIAFVKGAPKEVLQLCTQVLTRGKVVPLTEAVRSEIVAANDDYARRALRVLALARRDL
                     PARSGAYTIEKVEQGLTFLGLAAMMDPPRLDVAEAVKTCVGAGIRIVMITGDYGLTAE
                     SVARRVGMLTTDRPRIISGADIEAMSDADLQTALEQEVIFARMAPEHKLRVVAAFQAR
                     GDVVAVTGDGVNDAPALRKSDIGIAMGITGTDVAKEAADVILTDDRFAAIVSAIEEGR
                     AVYDNLRKFITYIFASNVPEIMPFVMMAIFHIPLALTVAQMLAIDLGTDLLPALALGT
                     EKPEPNVMNRPPRSRSQPVIDRGLLLRAFGWLGVIESALCFAGFLFVYSRLNGDLTLR
                     VDLLPWAQQVALPDGIIYILATTVYHAGVVSAQIGNAFACRTETERVFRLGLFSNRLL
                     LLGVAAEALLIAVMIYVPFVSPWFDHLPLPPVFWLIILINGPIL"
BASE COUNT          591 a          843 c          897 g          686 t
ORIGIN      
        1 ggcaatcact gcgcatgcaa gaatatcgct atggtgtctt gagcctgatc tcgcgtttct
       61 tcggcttgtt tggcctgcac aaaccggtgc gctatgcgcg acccgtcgaa gccggcacgt
      121 acgatacggc gttgcacacc gatcatcctg atgcgccctc tttcttggat caatacctcg
      181 atcagcctga gccgcatctc ggcggagccc cgaatgtaac ggccaagccc gacgaaactg
      241 aaaaattatg aggatgaagc caacaggatg aagtctggat tttctccttc atccttcatc
      301 acgttccgct ttccaaaatg aatctcgaac cgttacatgc tctgcgcccc accgatgttt
      361 tcaaagcatt ggaaactttt gctcagggtt taaccactgc tgaggccgaa tcgcgtctga
      421 cgctctatgg ccgcaatgca ttagccgaac cgcccgccgt tcctcaagcg cgaaaattta
      481 tcgggcatat cactcatccg atgggcttgc tgctgctcat cgccagcggg ccggctttca
      541 tcagcgggca tgtggtgctg ggcttcatca tcttgatcgt cgtcatcttc aatgcggtct
      601 tttcctattg gcaggaatat cgcgccgagc gcgcaattca cgccttgaaa caactcctgc
      661 cggtttacgc ccgcgtcatt cgtgatggag cggaaaccaa agtcccggcg gatacggtcg
      721 taccgggcga tgtgttagtc ctggcggaag gcgacaacat tcccgccgat gcgcgggttg
      781 tggaagaata tggactgcgc ctcaacaact ccacacttac aggtgaagct ttgcccgcgc
      841 gcaaaaccgc agatgcagcg ctgcgccgtg atgtgacaga ggtcgagcag cccaatctcg
      901 ttttcgccgg aacctcggtc gtgtccggca cgggccgcgc agtcgtgttt gccagcggca
      961 tgactacgca attcggccga atcgccaacc tcacccaaac agtcgagcaa gagcctagcc
     1021 tgattcagca gcagatgaag cgcatcacgc ggatgatcac ttactttgcg ctggggctgg
     1081 gcatcatcgt gctgttcgtc agcactgccg atctgaatct ccccgcgctc gatgccatta
     1141 ttctagcagt cgggatcatc gtcgctgtcg tgccggaagg tttgctgccg acggtcacgc
     1201 tatcgttggc gatcgcggtg cggcgcctgg cgcagcgcgg tgtgctcgtg aagcagttgt
     1261 cggtgatgga gacgcttggc aagacttcag tgatctgtac cgataaaagc ggcacgctca
     1321 cgcaaaatca aatgacggtg cgcgaagtgt gggcgagcga tcagcgcttg acggtgtcgg
     1381 gcgtgggcta tgaaccgaaa ggcgaattct cggcaatcgt cagcagtgat ctggatgcgt
     1441 tattgatcag cgcggctttg tgcaacaatg cgcgcttgac ttcgccgacg atcgatcatc
     1501 cgcactggac ggccttgggc gatcagactg aagcagcttt gcgcgtgatg gcgttgaagg
     1561 gcggcctgga tgaaagtgcg atcaactctc agtttccgcg tgtgcacgaa ctgcccttcg
     1621 atgcgcggcg taaacgcatg accaccattc atcgcacggc gcgcggcgag atcgcgtttg
     1681 tcaaaggcgc gcctaaagaa gtgcttcagt tgtgcacgca ggtattgact cgcggtaaag
     1741 tcgtcccgtt gacggaggct gttcgatcgg agattgtcgc cgcgaatgac gattacgcgc
     1801 ggcgcgcact gcgcgtgctg gctttagccc gacgggactt gcctgcccga tcgggcgcat
     1861 acacgatcga gaaagtggaa cagggtctaa cttttcttgg cttagcggcg atgatggatc
     1921 cgcctcgact ggatgtggct gaagccgtca agacctgtgt cggggccgga attcgcatcg
     1981 tcatgatcac gggcgattac ggactcaccg ccgaatcggt ggcgcggcgc gtcgggatgc
     2041 tgacgaccga tcggccgcgc atcatcagcg gcgctgacat cgaagcgatg agtgatgctg
     2101 atttgcaaac agcgcttgag caagaagtga tttttgcgcg catggcgccg gagcataaac
     2161 tgcgcgtggt tgccgctttt caagcgcgcg gcgatgtagt ggccgtcacc ggcgatggcg
     2221 tgaacgacgc gcctgcgctg cgcaaaagcg acatcggtat tgcgatgggc atcaccggca
     2281 ccgatgtggc gaaagaagcc gccgatgtga tcctgaccga tgatcgattt gcggccattg
     2341 tgtcggcaat tgaagaaggc cgcgcggtgt acgataatct gcgtaaattc atcacgtata
     2401 tttttgccag taacgtgccc gagatcatgc cctttgtgat gatggctatc ttccatatac
     2461 cactggcatt gaccgtagcg cagatgttgg cgatcgatct gggcacggat ttgctgcccg
     2521 cgctggcttt gggcacggag aaaccggaac cgaatgtcat gaatcgcccg ccgcgcagcc
     2581 gatcgcaacc ggtgatcgat cgaggtctgc tgctgcgcgc cttcggctgg ctcggcgtga
     2641 tcgaatcggc tttatgcttt gcgggttttc tatttgtcta ctcgcgcttg aacggcgatc
     2701 tgaccctgcg cgtcgatctg ctgccctggg cacaacaagt tgccctgccc gatggcatca
     2761 tttacatttt agcgacgacg gtctatcacg cgggtgtggt cagcgcgcag atcggcaatg
     2821 cgtttgcctg ccgcaccgaa accgaaagag tgtttcggct ggggctgttc agcaatcgct
     2881 tgttgctgct cggcgttgcc gccgaagcgc tgttgattgc ggtgatgatc tatgtgccgt
     2941 tcgttagtcc gtggttcgat catctgccgc tgccgccggt gttctggctg attatcttaa
     3001 tcaacgggcc gatccta
//
LOCUS       DASRTJ010000380         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_161527,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000380 DASRTJ010000000
VERSION     DASRTJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161527"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFF70_07650"
     CDS             <1..367
                     /locus_tag="VFF70_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44607.1"
                     /translation="AFVFWEGDAHRLWDGVTLIRCGGHFPGSTVLHWADGANGHGALL
                     TGDTITVVQDRRYVSFMYSYPNIIPLNAKAVRGIVQSIEPYAFDRIYGGWWDSIVTTD
                     AKAAVKRSADRYIKAISEH"
     gene            549..1151
                     /locus_tag="VFF70_07655"
     CDS             549..1151
                     /locus_tag="VFF70_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZY44608.1"
                     /translation="MSRNQSIQGVNLSRTIRKYLVSGFVVVTFVAYALHERSGNTDGV
                     ASAAAPKVQPAASTHQVTITPRPSPTASKLVFASPQVASTPTPVPQPTDTATPEPPSA
                     AAAGQYKDGTYTGPRVNVFYGLVQVQATVKNGQLADVQFLEYPSDRRTSQRINSIAMP
                     YLQQEAIQAQSANVNLISGATLTSEGFAMSLDNALASARN"
     gene            1154..1897
                     /locus_tag="VFF70_07660"
     CDS             1154..1897
                     /locus_tag="VFF70_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZY44609.1"
                     /translation="MKQTRLLMGMPVSVEVVDPRVTSADLDAVYNYFTYVDEKFSTYK
                     DTSEISAINRGELTPDRTSADMRTIFALAEQTREATNGYFDILHDGLYDPSGIVKGWA
                     IYHAAELLHRKGFKNFYVDAGGDVEAAGKNDQAAAWRVGIRNPFDTNEIVKVVGISNC
                     GIATSGTYIRGQHIYNPYKAAPLTDIMSITVIGPNVYEADRYATAAFAMGRDGIAFIE
                     NLTGFEGYMIDQNKQATFTSGFARYVIHA"
     gene            1890..>3164
                     /locus_tag="VFF70_07665"
     CDS             1890..>3164
                     /locus_tag="VFF70_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZY44610.1"
                     /translation="MLRAIDNLLNRITMYRLVLYVLIALAGIAVVLSAFGVMGYDPFA
                     LLFSIGFLLAACALANWSFAKAFKIPANAESTYISALILALIISPIQSYNDLWFLAWA
                     AVLAMASKYIVAINKRHIFNPVAFAVTITYFTINQSASWWVGDAPMLPFVLIGGLLIV
                     RKIARFELVISFFVSALISIVAFSLFNGDDVLVGLQRTIVYSPIMFFAFIILTEPLTT
                     PPNQKLRAIYGALTGFFFAPQIHFGSFYITPELAMLIGNVFSFVVSPKSTLILTLNDK
                     IRVAPDIYDFIFKPDRILAFIPGQYMEWTLGHDEPDSRGNRRYFTLASSPTEDHLRLG
                     VKFYPNGSSFKQALLKLDHHHEPVATQLAGDFVLPNDPNQKCVFIAGGVGITPFRSMI
                     KYLMDTRQRRSIVLFYVNRSINDIIYKDVFDKA"
BASE COUNT          706 a          869 c          812 g          777 t
ORIGIN      
        1 agcgttcgta ttttgggagg gtgatgcgca tcgcttgtgg gacggtgtga ccttgattcg
       61 ctgcggcgga cattttcccg gctcgaccgt gctgcattgg gcggatggcg caaacgggca
      121 cggcgcgttg ttgaccggcg atacgatcac cgtcgttcaa gatcgccgct acgtcagttt
      181 catgtacagt tacccgaaca tcattccgct gaatgcgaag gcagtgcgcg gcatcgtgca
      241 atcgatcgag ccgtatgcgt tcgatcggat ttacggcggc tggtgggatt cgattgtgac
      301 gactgatgcg aaggcggcgg tcaagcgatc cgccgatcga tatatcaaag cgatctcgga
      361 acactgaaat gtgaatttgt tgttaagact tgcggtcgat catcttcgaa aaaatacgaa
      421 cccaccgaat gacaacgctt ctttagactc tcttcatctg ggcttcataa agattcgcta
      481 agatacaatt acctcgcgct gagcgtgagg tgttttttat ggcctagcga tcttatcagg
      541 agcagagcat gtctagaaat caatccattc aaggcgtcaa tcttagccgc acgatccgaa
      601 aatatcttgt atccggtttt gtcgtcgtca ctttcgtcgc atacgcgctg cacgaacgat
      661 cgggcaatac ggatggcgtc gccagcgcgg ctgcgccaaa ggtacaaccg gcagcctcta
      721 ctcatcaagt cactatcaca ccgcgaccgt cgccgaccgc ttcgaaactg gtcttcgctt
      781 ctccgcaggt cgcatcgacg ccgacgccgg ttccacaacc gacggacacg gccacaccag
      841 aaccgccatc ggccgccgca gccggtcaat acaaagatgg aacgtatacc ggcccgcgag
      901 tcaatgtctt ctacggtctc gtgcaagttc aagcgaccgt caagaacgga caactggccg
      961 atgtgcagtt cttagaatac cccagtgatc gacggacttc gcagcgcatc aattcaatcg
     1021 ccatgccgta tctgcagcaa gaagccatcc aagcacagag cgccaatgtc aatctcattt
     1081 cgggcgcgac cctgaccagc gaaggctttg ccatgtcgct agacaatgca ctcgcctcag
     1141 cgcgcaacta atcgtgaaac aaactcgttt attgatgggc atgcccgtca gcgttgaagt
     1201 ggttgatcca cgcgtcactt cagctgatct tgatgcggtc tataactact tcacttacgt
     1261 cgatgagaag ttcagcacgt acaaagacac cagcgagatc tcggcgatca accggggcga
     1321 actcacgcct gatcgaacga gcgccgacat gcgcacgatc tttgcgctgg ccgagcagac
     1381 tcgcgaagcg accaacggct acttcgatat tctgcacgac ggcttatacg atccatcggg
     1441 catcgtcaaa ggctgggcga tctatcatgc ggctgaactg ctgcatcgaa aaggctttaa
     1501 gaatttctac gtcgatgcgg gcggcgatgt cgaagcggcc ggcaaaaacg atcaagccgc
     1561 agcgtggcgc gtgggcattc gcaatccatt cgacaccaat gaaattgtca aggtcgtcgg
     1621 catcagcaat tgcggcatcg ccacttccgg cacttacatt cgaggccagc atatttacaa
     1681 tccgtataaa gctgcaccgc tcaccgacat catgagcatt acggtgatcg ggccgaatgt
     1741 ttacgaagcc gatcgatacg ccacggccgc cttcgccatg ggccgtgacg gtattgcctt
     1801 cattgaaaat ttaacgggct ttgaaggtta catgatcgat caaaataagc aggccacttt
     1861 caccagcggc tttgcgaggt acgtcattca tgcttagggc tatcgacaac ttgctcaacc
     1921 gcatcaccat gtatcgattg gtgctgtatg tcttgatcgc attggcaggc attgccgtgg
     1981 tgctgtcggc gttcggcgtc atgggctacg atccgttcgc gttgttattc tcgatcggct
     2041 ttctgctcgc ggcgtgcgct ctcgccaatt ggagttttgc caaagcattc aaaattccgg
     2101 ctaacgccga atcgacttat atttcagcct tgatcctcgc attgatcatc tcgccgatcc
     2161 agtcgtacaa cgatctatgg ttcttagcct gggcggcggt tctggccatg gcttcgaagt
     2221 acatcgtcgc gatcaacaag cggcacatct ttaatcccgt tgccttcgca gtcaccatca
     2281 cctacttcac gatcaatcaa tcggcgagtt ggtgggtcgg cgacgcgccg atgctgccgt
     2341 ttgtgttgat cggtggattg ctgatcgtgc gcaagatcgc tcgctttgaa ttagtcatta
     2401 gtttctttgt gagtgcattg atctcgatcg ttgctttcag cttattcaac ggcgatgatg
     2461 tcctcgtcgg cctacagcgc acgatcgtgt attcgccgat tatgtttttc gccttcatca
     2521 ttctgactga gccgttgacc actccgccca atcaaaagtt gcgcgcgatc tatggtgcgc
     2581 tcaccggctt tttctttgcg ccgcaaattc atttcggcag tttctacatc acgcccgaac
     2641 tggcgatgct gatcggcaac gtcttctcat tcgtcgtcag ccccaaatcg acgttgattc
     2701 taactttgaa tgacaagatt cgagtggcgc ccgatattta cgatttcatc ttcaagcccg
     2761 atcgcatact cgccttcata ccggggcaat atatggaatg gacgttgggt catgacgaac
     2821 cggacagccg gggcaatcga cgctatttca ctctagcgtc ctcaccgact gaagatcatt
     2881 tgaggctggg cgtgaagttt tatccgaatg ggagttcgtt caagcaggcg ctgctcaagc
     2941 tagatcatca tcacgagccc gtcgcgacgc aattagcggg tgactttgtg ctgcctaatg
     3001 atccgaatca gaagtgtgtg ttcatcgcgg gcggcgtggg tatcacgccc tttcgcagca
     3061 tgatcaagta tttgatggac actcgccaac ggcgatcgat cgtcttgttc tacgtcaatc
     3121 gatcgatcaa cgacatcatt tacaaagatg tgttcgacaa ggct
//
LOCUS       DASRTJ010000381         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_61631,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000381 DASRTJ010000000
VERSION     DASRTJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61631"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..550
                     /locus_tag="VFF70_07670"
     CDS             35..550
                     /locus_tag="VFF70_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44611.1"
                     /translation="MLSGDNIINPFCVHPCPCGYHGDPTHNCTCSPSMVTRYQKRISG
                     PLLDRFDIHIEVPRVNYEKLTADRLGETTEKVRSRVVAARERQADRFIGTSLICNADM
                     TASHIREFCVVDEAGKSLMRAAMQQLGMSARSFHRVLKLARTIADLADSDQIQSAHLA
                     EALQYRPRQQT"
     gene            complement(615..1103)
                     /locus_tag="VFF70_07675"
     CDS             complement(615..1103)
                     /locus_tag="VFF70_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44612.1"
                     /translation="MLRATKAVPGAWTDEITSLPTAQQAVEKLTLKFSNGLRIYGLLP
                     GEKLEDLVFQSGDWKRWLFLFRRPVLANTLLLLSSNYTVVIREELEVAQGWIISYIPR
                     SSIVGIQNQPRSLWKELTVQLNRGDQTAEYKLRLTDEAAQAWREQWIQHGGQWQDLPD
                     DA"
     gene            complement(1225..2256)
                     /locus_tag="VFF70_07680"
     CDS             complement(1225..2256)
                     /locus_tag="VFF70_07680"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HZY44613.1"
                     /translation="MLNFPHRRFNPLTGEYVLVSPHRAGRPWLGQVEKSPSDQRAAYD
                     PDCYLCPGNQRASDARNPLYTETFVFTNDFAALLQQASEMPKTSQPLLKVESEPGNCR
                     VICFSPRHDLTLAEMPTHAIRTVVDVWVTQTSELGQQYRWVQIFENKGEMMGSSNPHP
                     HGQVWAGSALPNEPAKEDRQQHAYFEERHSVLLVDYARLESDQRERIVVENDRWMVVV
                     PFWALWPFETLLLPRRQVLRLPDLTGEDRDSLAQILKHLLTKYDNLFESSFPYSMGWH
                     GAPFDPGDQPHWQLHAHFYPPLLRSAVVRKFMVGYELLAEAQRDLTAEQAADRLRALS
                     EIHYKKSAV"
BASE COUNT          602 a          673 c          696 g          569 t
ORIGIN      
        1 gagataacct gcagttctga tttcccatca aaagatgctg tcaggtgata acataataaa
       61 cccgttttgt gttcatccct gcccgtgcgg ctatcacggc gaccccacgc acaactgcac
      121 gtgttcaccc tcgatggtca cgcggtatca aaaaagaatc tccggcccgt tgctcgatcg
      181 cttcgacatt cacatcgaag tgccgcgtgt gaactatgag aagttgaccg ccgatcggtt
      241 gggggaaacg acagagaaag ttcgatcgag agtggtggcc gcgcgtgagc ggcaggctga
      301 tcgattcatc ggcacgagtt tgatctgcaa tgcggacatg accgcgtcac acattcgcga
      361 attctgcgtc gtcgatgaag cggggaaatc gttgatgcgc gcggcgatgc agcagttggg
      421 catgagcgcg cgatcgttcc atcgagtctt gaagttagcg cgcacgatcg ccgatcttgc
      481 cgactccgat caaatccaat cggcgcattt ggcggaagcg ctgcagtatc gaccgaggca
      541 gcaaacgtga tcgaggaacg tcatatggag gctgcgctgc acttatgatt gttctgagcc
      601 tagtagcgcg acgatcatgc atcatctggg aggtcttgcc attgaccgcc atgttgaatc
      661 cactgctcgc gccaggcttg ggcagcttcg tccgttagcc gtaatttata ctcggccgtt
      721 tggtctcctc gattcaactg aacagttaat tccttccata ggctgcgtgg ctgattctgg
      781 attccgacaa tactgctccg aggaatatac gagatgatcc agccctgcgc aacctccaat
      841 tcctccctaa tcaccacggt gtaattgctg ctgagtaata acagcgtatt agccaaaacg
      901 ggccggcgga ataaaaacag ccagcgcttc cagtcgccgg actggaagac caagtcctcc
      961 aacttttcgc caggtagtaa gccatagatg cgcaggccat tggaaaactt cagcgtcaat
     1021 ttttcaacag cctgttgcgc agtcggcaag gaagtgattt cgtctgtcca tgcgccgggc
     1081 acagctttgg tcgcccgcag caattgccgc gcggcccggt gcgcgcacga ggatttgagg
     1141 tggggcggtg aaacgccgct cgaattcagc gagaacctgt tgttgggcag acattcgaaa
     1201 ctccaattga aagtacaacg agtcttagac ggcagacttt ttgtagtgaa tctcggacag
     1261 agcgcgtaat cgatcggccg cttgctcagc cgtgagatcg cgttgggctt cagccagcag
     1321 ctcgtagcca accatgaact ttctcacgac ggccgatcgc aacaagggcg gataaaaatg
     1381 ggcgtgcagc tgccagtgtg gttgatcgcc cggatcgaat ggggcgccgt gccagcccat
     1441 cgagtacgga aaggaactct caaagagatt atcatacttc gtcagcaagt gtttgagaat
     1501 ctgggcaaga ctatcgcgat cttcgccggt gagatcaggc aggcgcaaca cttggcggcg
     1561 gggcagcaac agtgtttcga aaggccacag tgcccagaac ggcacgacga ccatccagcg
     1621 atcattctcg acgacaatgc gctcgcgttg atcgctctcg agccgggcat aatcgaccag
     1681 caggaccgaa tgacgttctt caaaataggc atgttgctgc cgatcttctt tcgcgggctc
     1741 gttaggtaga gcgctacccg cccaaacctg tccatgagga tgcgggtttg aactgcccat
     1801 catctcaccc ttgttctcga atatctgcac ccagcgatat tgctgaccca gttctgacgt
     1861 ctgggtaacc cagacgtcaa cgacggtgcg aatagcgtgc gtcggcattt cagctagcgt
     1921 gagatcgtgc cgtggcgaga aacagatcac gcgacagttg cccggttcac tttccacttt
     1981 gagcaagggc tgagatgtct tcggcatttc actggcctgt tgcaacagtg cggcaaagtc
     2041 gttcgtgaac acaaaagttt ccgtgtacag tgggttacga gcatcactgg cgcgttgatt
     2101 acccgggcac agatagcaat ctggatcgta agctgcacgc tggtcgctcg gcgatttttc
     2161 aacttgaccc agccatggac gcccagcacg atgcggggaa actaacacgt actcaccggt
     2221 taaggggtta aagcgacgat gtggaaagtt caacattgat tcaaagttcg atcgcgaggc
     2281 taggcctgac gtacaaaaat cttcaaacca atcttaacga agttattgat cggtgacatc
     2341 aatggcctag ctcgtgaaga gccgctctgg aggcttgtag tctatctcac cgcataaggc
     2401 cggtctgcgc gagtgaggaa tgtcatcttc aatatggata aacgatgata tcgcagagat
     2461 gattctctgc gatgtcgcca catcaaacaa cctaacccca agcgggttgc aatagcttgg
     2521 tttgactgga tatgtcgacc
//
LOCUS       DASRTJ010000382         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_161543,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000382 DASRTJ010000000
VERSION     DASRTJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161543"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFF70_07685"
     CDS             <1..367
                     /locus_tag="VFF70_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44614.1"
                     /translation="AFVFWEGDAHRLWDGVTLIRCGGHFPGSTVLHWADGANGHGALL
                     TGDTITVVQDRRYVSFMYSYPNIIPLNAKAVRSIVRSIEPYPFDRLYGGWWDSIVPTD
                     AQAVIHRSADRYIKAIAAD"
     gene            complement(345..1424)
                     /locus_tag="VFF70_07690"
     CDS             complement(345..1424)
                     /locus_tag="VFF70_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44615.1"
                     /translation="MDDHPPFPHDQLTAAIDALLDRNPRVFSIDRWSIHGVRFEFHVD
                     HPLLRDLMRSMLHSFPIDPTGPTDVRMCLTTYDRWSIPMEAEIFPSQANEIIDLKAAY
                     GLTPKFGRWQALNFYELTPLGGVVYQLKAGQALGYVHDPASFHPWFISHLLMQLIVLD
                     LLRSQDLFWMHAACVADHDRGVLLAGQSGSGKTTTCLRLIEGGFKFLSEDRTFFRSVG
                     GAIELLSFIEAVSVTQNSLKLLPALSKQIGSQEFGARYKIDLDPAQLFPNALLDRASP
                     GLILFPRVTSSEQAATRRLNSAGALQMILPSSLLASQPDVSGLHFDALTQLVTSSRCY
                     ELLLGRDVDRLPELVRGLLNQPQSL"
     gene            complement(1668..1952)
                     /locus_tag="VFF70_07695"
     CDS             complement(1668..1952)
                     /locus_tag="VFF70_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZY44616.1"
                     /translation="MTIELRPHDRFTPSPDVKVNVIDGEAVLLDLRTGAYFGLNKVGA
                     HIWTLYGEGQSVHQAIEGVCQRFEVDAVRAERDTRALTEKLVERGLLRLA"
BASE COUNT          477 a          552 c          576 g          422 t
ORIGIN      
        1 agcgttcgta ttttgggagg gtgatgcgca tcgcttgtgg gacggtgtga ccttgattcg
       61 ctgcggcgga cattttcccg gctcgaccgt gctgcattgg gcggatggcg caaacgggca
      121 cggcgcgttg ttgaccggcg acacgatcac cgtcgttcaa gatcgacgct acgtcagttt
      181 catgtacagt tacccgaaca tcattccgct gaatgcgaag gcagtgcgca gcatcgtgag
      241 atcgatcgag ccgtacccgt tcgatcggct ttacggcggc tggtgggatt cgatcgtgcc
      301 gacggatgcg caggccgtga tacatcgatc ggccgatcgc tatatcaaag cgattgcggc
      361 tgattgagaa gacctcgcac caattccggc aatcgatcga catcgcgacc caatagcaat
      421 tcgtaacagc gtgatgaagt gaccagctgc gttagcgcat caaagtgcaa tccgctgaca
      481 tccggttgcg aggctaataa actgctgggc aaaatcattt gcagggcacc ggcggaattt
      541 aagcgacgcg tggcggcttg ctcagaagag gtgacacgcg gaaagagaat caaacccggc
      601 gaggcgcgat ccagcagcgc gttcggaaac aactgcgccg gatcaagatc gatcttgtag
      661 cgcgcgccaa attcctgtga cccgatctgt ttactcagtg cgggcagcaa cttcaagctg
      721 ttctgtgtaa ccgatacggc ctcaataaat gacagcagct cgatcgcacc accgactgat
      781 cggaagaacg ttcgatcttc tgacaagaac ttgaagccac cttcaatcaa ccttagacac
      841 gtcgtcgttt tgccggagcc ggactgcccc gccagcagca cgcctcgatc gtgatcggcc
      901 acgcaggccg catgcatcca aaacaaatct tggctgcgca acaaatccaa cacgatcaac
      961 tgcattaaca aatggctgat gaaccacggg tgaaatgatg ccgggtcatg cacgtaacct
     1021 aaggcttggc cggccttcag ctgatacacc acgccgccaa gaggagttaa ttcatagaag
     1081 ttcagcgcct gccagcggcc aaacttcggc gtcaagccgt aggctgcttt gagatcgata
     1141 atttcattgg cttgggacgg gaaaatctcg gcttccatcg ggatcgacca acgatcgtat
     1201 gtcgtgaggc acatgcgaac gtccgtcggg ccggtgggat cgatcgggaa agagtgcagc
     1261 atcgatcgca ttaaatcgcg cagtagtgga tgatcgacat ggaattcaaa gcggacgccg
     1321 tggatcgacc agcgatcgat ggagaaaacg cgcggattgc gatcgagcag cgcatcaatc
     1381 gccgcggtca gctggtcgtg aggaaatggc gggtgatcat ccatgcagaa aaaccgagca
     1441 gcccgcgtat gagctgtagc gctggccacc agcccacacg ggctgctgga atttgaccga
     1501 caggtgatgc gccccaggca tctataattc cgaggacgcc gcagtgatat cggtcaaggc
     1561 tggatactca accagctctg gtgtcacata cgtttctttc atcttgatcg ccccctcgcc
     1621 gtgttggatg gtttagccct ttacaggtca cgacttcttc cgcaggtcta ggccaggcgc
     1681 aacaaaccgc gctcgactaa tttttcggtg agtgcgcgcg tgtcacgttc agcgcgcact
     1741 gcatcaactt caaagcgctg gcagacacct tcaatcgctt ggtgcacaga ctggccttca
     1801 ccatacaacg tccaaatgtg cgcgccgact ttattcagtc caaagtaggc gcccgtgcgg
     1861 agatcaagca agacggcttc gccgtcaatg acgttgactt taacatccgg ggaaggtgta
     1921 aatcgatcgt gagggcgaag ttcgatggtc atgggctggg agtcagttca agttgctgcg
     1981 actgtaacat catttgcaga cccggtcaaa cccgatcggt ttggctg
//
LOCUS       DASRTJ010000383         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_250732,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000383 DASRTJ010000000
VERSION     DASRTJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250732"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /gene="nuoB"
                     /locus_tag="VFF70_07700"
     CDS             complement(<1..414)
                     /gene="nuoB"
                     /locus_tag="VFF70_07700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZY44617.1"
                     /translation="MSQPEAFENDPMVPAELHNNVFITTLDKLYNWGRKRSMWPMMFG
                     LACCAIEMICTAASRYDLERFGAGLMRPSPRQSDLMIVSGTVTKKMVPTIVRLYNQMA
                     EPKYVLSMGACATGGGPFKEGYNVVSGIDKFLPVDV"
     gene            complement(401..616)
                     /gene="iscX"
                     /locus_tag="VFF70_07705"
     CDS             complement(401..616)
                     /gene="iscX"
                     /locus_tag="VFF70_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HZY44618.1"
                     /translation="MELFWEDDYAIAKALMRAYPQIDPLDVELETLHQWIIELPEFSD
                     DKSIQAIYLLEAIQTEWYEEVASHEST"
     gene            complement(674..>1541)
                     /locus_tag="VFF70_07710"
     CDS             complement(674..>1541)
                     /locus_tag="VFF70_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZY44619.1"
                     /translation="SQALVAHPLVQVISFTGSTNTGRSIGAEAARLGKKVSLEMGGKN
                     AIIVMDDANLDLALEATLWAAFGTTGQRCTAASRLIIQRKVHRQFVDRLLDRTRQLRL
                     GDGLNPKVDVGPLINQVALTKVSGYVEIGKQEGAHAIVGGNVHSNGGKGFFYEPTLFD
                     QVTPSMRVAQEEIFGPVLSIIEFDRLDEAIKINNNTLYGLSTSIFTENVNDAFRSMRD
                     INSGIVYINHGTTGAEIQFPFGGTRGTGNGHREAGQVGIDVFTEWKAIYVDYSGKLQR
                     AQIDQSGFGVAE"
BASE COUNT          351 a          449 c          362 g          379 t
ORIGIN      
        1 cacatcgacc ggcaggaatt tatcgatgcc cgacacgacg ttgtagcctt ctttgaaggg
       61 tccgccgccg gtcgcgcatg cgcccatcga cagcacatac tttggctcgg ccatctgatt
      121 gtacaatcgc acgatcgtcg gcaccatttt cttggtcacc gtgcccgata cgatcatcaa
      181 atcactctgt cgcggtgagg gccgcatcag cccagcgccg aatcgttcca agtcgtagcg
      241 gctcgccgcc gtgcagatca tttcgatcgc gcagcaggcc agaccaaaca tcatcggcca
      301 catcgatcgc ttgcggcccc agttgtacag tttgtccaac gtcgtgatga atacgttgtt
      361 gtgcagttcg gcgggaacca tgggatcgtt ttcaaatgct tcaggttgac tcatgcgagg
      421 ctacctcctc ataccattct gtttgaatcg cttccaacaa ataaatcgct tgaatagatt
      481 tgtcgtcact aaattcgggc agttcgatga tccactgatg cagcgtttcc aactcaacgt
      541 ccagcggatc gatttgggga tacgcgcgca tcaaagcttt ggcaattgca taatcatctt
      601 cccaaaataa ttccatatcg atcggtcaac gcgcgctatt ttatcacagt tcgattcgat
      661 atgcgacgca gattcattca gccacgccaa aacccgattg atcgatctgc gcccgctgga
      721 gcttgccgct gtagtccaca tagatcgctt tccattcggt gaacacatcg atgcccacct
      781 gcccggcttc gcgatgacca ttgcctgtgc cgcgcgtccc gccgaacgga aattgaatct
      841 ccgcgccggt tgtgccgtga ttgatgtaaa caatgccgct attgatgtcg cgcatcgatc
      901 gaaacgcgtc attcacattt tcagtgaaga tgctggtcga taaaccatag agtgtgttat
      961 tgttgatctt gatcgcttca tcgagccgat cgaattcgat gatcgataac accggcccga
     1021 aaatttcttc ctgcgccacg cgcatcgatg gagtaacttg atcaaacaac gtcggctcat
     1081 agaaaaatcc cttgccgccg tttgagtgaa cattgccccc gacgatcgcg tgcgcgcctt
     1141 cttgtttacc gatctcaaca tagccgctga ctttggttaa agccacttga ttgatcaatg
     1201 ggccgacatc aactttggga ttaagcccat cgcctaacct caattgccgc gttcgatcga
     1261 gcaaccgatc gacaaattgg cgatgcacct tgcgctggat aatcaatcgg gaagcagccg
     1321 tacatctttg acccgtcgtg ccgaacgccg cccacaacgt cgcctcgagt gctaaatcga
     1381 gattggcatc gtccatcacg atgatcgcat tcttgccgcc catctccagt gaaactttct
     1441 tgcccagccg cgccgcttcc gcgccgatcg atcgaccggt gttagttgaa ccagtaaatg
     1501 aaatgacttg caccaaggga tgtgcgacca atgcctgact g
//
LOCUS       DASRTJ010000384         6678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_161546,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000384 DASRTJ010000000
VERSION     DASRTJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161546"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFF70_07715"
     CDS             <1..286
                     /locus_tag="VFF70_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44620.1"
                     /translation="YVVMAHRIETKKGKWAWAGLGWTQDPSDMRGLFVEQSGSSEEEV
                     RTNTISTVQSLMDRRPYTYGSIHTEIAGIECTGIPVCVMVVAAYTSRGWK"
     gene            290..1087
                     /locus_tag="VFF70_07720"
     CDS             290..1087
                     /locus_tag="VFF70_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZY44621.1"
                     /translation="MAKKGSSVKQKIIIAGESPFVEDLSALCVDAGHDVAMYLVEDLV
                     ESNALRRLADDATTANVAIDVMIDSIETKRDLVRALDRSLPADAPLLSASMSVTATQI
                     GAWLSHPDWVVGFGLLPPLKKGSLIELAAGLQTDPSTLGKAKVFFEGLGLETAVVEDS
                     VGLVLPRIVCNLVNEAAYALMENVAQPHDIDTAMKLGTNYPHGPLGWGDLIGLDIVLA
                     VLRGLHEEYGDDRYRPAPLLKQMVRAGQLGKKSGKGFYDYSEMTADR"
     gene            complement(1122..2324)
                     /locus_tag="VFF70_07725"
     CDS             complement(1122..2324)
                     /locus_tag="VFF70_07725"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZY44622.1"
                     /translation="MSDDQLLISNLLSAFRNSFHSDPEIVVRAPGRVNLIGEHTDYND
                     GFVLPVAIDRYVYLAASKCVSHLTTIRSIDFDQSATFRITELEPKSDRGWITDRTLWA
                     NYPAGVAWALRESGSIFTGFDAAFTSTVPIGAGLSSSAAVDVAFATTWKKLYGLEIED
                     AALAKLCQRADNDFVGIACGIMDQLISVLGSRDDALFIDCRSLKTENVPLPKNVAIVV
                     ADTKVKRSLASSAYNERRSQCEEAASILRRADDSILALRDVSPVLLLARQADLPPIIF
                     RRARHIVQENHRVLLAVDALRRGDLIEVGRLMKESHLSLRDDYEVSCQELDAMVEAAW
                     SIEGVIGSRMTGAGFGGCTVSLVQIDQADRFVEELHDAYKNKTGIEPAIYVCHASNGA
                     AELTRDHS"
     gene            complement(2317..4044)
                     /locus_tag="VFF70_07730"
     CDS             complement(2317..4044)
                     /locus_tag="VFF70_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HZY44623.1"
                     /translation="MRTLRQCLLDTDAALLRVIASRWSIDATGLKPRDLVARVESELS
                     QPARASKMMEVLSPAERDAFRSLLAAAGTLPAINFAQRFGSIRPVGPARLEREQPWRS
                     PVSPAEGLWYLGFIYRGFEQLPNGAMREVYFAPQEFVPLLPLLKVSAPPLEPLPLSAP
                     PAQVESIDDQLADDLCTLLDHLHNNFVRISGSLKISIDYAQLDRLLHRSDAAWIDFII
                     RLAIRARLLKIDNHRLRPDPKPAADWLQATARDQLRILFEAWRTDPQSTLTALPLVLE
                     PKGAWIKDPIAAGAAILAALRGAVPNTWHAVQALPDRMQSNSPDFARSEFDTGYIKNT
                     VTAEYLRGFEAWNQVEGELIRYILSQPLHWLGMIDLGSSDPTSNIQLPISNLQSLTSN
                     FVSHPLSFRLSHIGASLLGLESDEAAKSMHRESHFIIHPNATIDVVAARRYDRFQLAR
                     IANLIDRRKGEYIYRITPSSLARAQSQKITSAKAIEFLAHAAGRVMPPSVIKAIERWT
                     KKGAEVKVERAVLVRVKDAAILKRLQESPKTKSIAIEVLGPTAARINERDWPKLAAIL
                     AESGVLVDE"
     gene            4182..4661
                     /locus_tag="VFF70_07735"
     CDS             4182..4661
                     /locus_tag="VFF70_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44624.1"
                     /translation="MLVVGIYAFCSCAFNLFLNWYDTGAINSEGWLPTVIGGAVFGLI
                     AVSIERRIYYQRQELWPQKRLLFYVRLIILSIFYVDLGLGFGSGFDYMLREIFAGTTF
                     FVALKDLLLVPAIVVFYWLMILPFGLTAGLLMGLLLRSNLSAQSFREDQVDYIESLG"
     gene            4712..5395
                     /locus_tag="VFF70_07740"
     CDS             4712..5395
                     /locus_tag="VFF70_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY44625.1"
                     /translation="MPAHSELLFHLTRPPLQKDSAAPPLLLLLHGVGSNEEDLFELSK
                     YLDPRFFIVSARAPIQLMPGAYAWYHVQFLPDDMRIDFREVEHSRSILQRFIAELIES
                     YQVDPDRVYLMGFSQGAIMSLSLALTEPDRLAAIVAMSGRLLPELTPQIAAPEMLKDF
                     PIFMAHGLADNVIPIRDARAAHEQLSKLPVDLTYREYPMGHQVSIESLNDIAAWLKDQ
                     LDRKPASKA"
     gene            5467..6600
                     /locus_tag="VFF70_07745"
     CDS             5467..6600
                     /locus_tag="VFF70_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44626.1"
                     /translation="MINSRLLSIMRKEIIQITRDPRTLVISFMMPILMLFLLGFAATS
                     DLRNVPAVLFDQDHSKAARDLLDAYRAADYFSFDFEVDSAQALRQLIDSGQAKAGLII
                     PPDYGQHIASGETASVAFVIDGSDPTVASTALSAAMLIGQTKSTAVLRATLDARGQSN
                     AAGSPIEVRTQVWYNPDLVSAYFMIPGLMGTILLQMAVLLTSSAIVREREYGTIEQLI
                     ITPIRSWELIIGKLTPYVFIAFFDLLEVLVLGTLVFKVPMAGNVGLLLLMSALFLATA
                     LGIGLFISTVAKTQQEALMLGALIQLPSFFLAGFFFPIAAMPPFLQAISYLIPLRYYL
                     TIVRAIILKGVGIESIAGDVIPLAIFGVLIVTAAVLRVRKRLD"
BASE COUNT         1453 a         1713 c         1876 g         1636 t
ORIGIN      
        1 gtatgtggtc atggcgcacc gcatcgaaac taaaaaaggc aaatgggcct gggccggttt
       61 gggctggacc caagacccga gtgatatgcg cggactgttc gtcgaacaat cgggatcgag
      121 tgaagaagag gtgcgcacga atactatttc cacggttcag tcgttgatgg atcgccggcc
      181 atacacttac ggatcgattc ataccgaaat cgccgggatc gaatgcaccg gcataccggt
      241 gtgtgtcatg gtcgtcgcgg cttacacatc gcgcggctgg aagtaatgaa tggctaaaaa
      301 gggttcgagt gttaaacaaa aaattattat cgccggtgaa tcgccattcg tcgaagatct
      361 gtccgccttg tgtgtcgatg cgggtcacga tgtggcgatg tatctcgtcg aagacctggt
      421 cgaatcaaac gcattacgtc gattagcaga cgatgcgacg acggcgaacg tggcgatcga
      481 tgtgatgatc gactcgatcg agaccaaacg cgatctggtg cgcgccctcg atcgatctct
      541 gcccgccgat gcgccgctgc tctcggcttc catgtcggta accgcgacgc agatcggtgc
      601 gtggctctca catcccgatt gggtagtcgg ttttgggcta ttgccgccgt tgaagaaagg
      661 cagtctgatc gagctggcgg ccgggctgca aactgatcca tcgacgttgg gcaaagcgaa
      721 agtatttttt gaagggcttg gtttggaaac agcagtggtt gaagattccg tcgggttggt
      781 gctgccgcgg attgtgtgta atttagtgaa tgaggccgcg tatgcgttga tggaaaacgt
      841 tgcgcagccg cacgacatcg acacggcgat gaaactcggt acaaattatc cacacggtcc
      901 gctgggctgg ggcgatctga tcggcttgga cattgtgctg gcggtcttgc gcggcttgca
      961 tgaagagtac ggcgacgatc gctatcgccc cgctccgctg ctcaagcaaa tggtgcgggc
     1021 gggacaattg gggaagaaga gcggcaaagg gttttacgat tatagtgaga tgaccgcaga
     1081 ccgttgacga cggacaacag tagattatga gtcaacatgg atcaactgtg gtctctggtc
     1141 aattcagccg caccattgga tgcatggcac acatagatcg ccggctcaat gccggttttg
     1201 tttttgtaag cgtcatgcag ttcttccaca aatcgatcgg cttgatcgat ctgaacaaga
     1261 ctgaccgtgc aaccgccgaa acccgcgccg gtcatgcgtg aaccgatcac gccctcgatc
     1321 gaccaggcgg cttctaccat cgcatcaagt tcctgacaac tgacttcgta atcatcgcgc
     1381 aaacttaaat gagattcttt catcaagcga cccacttcga tcaagtcgcc gcgccttaag
     1441 gcgtctacgg ccaacaacac ccgatggttt tcctgaacga tatggcgcgc gcgtctgaat
     1501 ataattggcg gcagatcagc ctgtcgggcg agtaacaaca ccgggctcac atcacgcagc
     1561 gctaagatcg aatcgtcggc gcgtctcaag atcgatgcgg cttcttcgca ttgcgatcgt
     1621 ctttcattgt aggctgatga tgcgagtgat cttttcactt ttgtatccgc gacgacaatg
     1681 gcgacatttt tgggaagcgg cacgttttcc gttttcaatg atcgacagtc tataaataac
     1741 gcatcatctc tactgcccaa cacgctgatc agttgatcca taatgccgca ggctatgccg
     1801 acgaaatcgt tgtctgcgcg ctggcataac ttcgctaaag ctgcatcttc aatttcaagt
     1861 ccataaagtt ttttccacgt agtcgcaaag gctacgtcga cggcggcgga tgaactcaag
     1921 cccgcaccga ttggcacggt cgaggtaaag gccgcatcaa agccggtgaa gatcgatcca
     1981 ctctcgcgca gcgcccaagc cacgcccgcc ggataatttg cccagagagt tcgatcggtg
     2041 atccagcctc gatcggattt tggctccagc tcagtgatgc ggaaggtggc tgattgatcg
     2101 aaatcgatcg atcggattgt ggttaagtgg ctgacacatt tggatgcggc gagatacaca
     2161 tatcgatcga tcgcaacggg caggacgaag ccgtcgttat aatcggtgtg ctcgccaatc
     2221 aagttcacac ggccgggcgc acgaacgacg atctccggat cagagtgaaa tgaattgcga
     2281 aacgctgaca acagattgct gatcaacaat tgatcatcac tcatctacca gcactcctga
     2341 ttcggccaga atcgccgcga gtttaggcca atcgcgttcg ttgatgcgcg cggccgtggg
     2401 gccgaggact tcaatcgcga tcgatttagt tttgggcgat tcttgcagac gtttcaagat
     2461 ggcggcatct ttgacgcgca cgagcacggc acgctcgact ttcacttccg cgcccttctt
     2521 ggtccaacgc tcgatcgctt tgatcaccga aggtggcatc actcggccag cggcatgcgc
     2581 caggaattcg atcgctttgg ccgaagtgat cttttgcgat tgtgcccgcg ctaatgagga
     2641 tggggtgatt ctataaatgt attcgccctt acgtcgatcg atcaagttgg cgatgcgcgc
     2701 cagttgaaat cgatcgtagc ggcgagcggc gaccacatcg atcgtggcgt tggggtgaat
     2761 gatgaagtga ctctcgcgat gcatggactt tgcagcttca tcagattcga gaccaagcag
     2821 tgaagcgcca atatgggaaa gccggaagga taaaggatga gagacgaaat tggaagttag
     2881 agactggaga ttggagattg gaagttggat gttggacgtt ggatcggatg aacctaaatc
     2941 gatcatgcct aaccaatgca atggttgcga gagaatatac cggatcaatt cgccttccac
     3001 ttgattccac gcttcaaagc cgcgtaaata ctcagccgtt actgtgttct tgatgtaacc
     3061 cgtatcaaat tccgatcgag cgaagtccgg cgagttggac tgcatgcgat cgggcagcgc
     3121 ttgaactgca tgccacgtat ttggcactgc gccgcgcagc gcggccaaga tcgccgcacc
     3181 ggccgcgatc ggatctttga tccatgctcc tttcggctca aggaccaggg gcagcgcagt
     3241 caaggtcgat tgtggatcgg tgcgccaggc ttcaaataag atgcgcagtt gatcgcgcgc
     3301 tgtagcttgc agccaatcgg cggcgggttt gggatcgggc cgcagtcggt gattgtcgat
     3361 cttcagcagg cgggcacgaa tcgctaagcg gatgatgaaa tcgatccacg ccgcatccga
     3421 tcggtgcaac aaccgatcga gctgggcgta atcgatcgag attttgagcg agccggaaat
     3481 acgcacgaag ttgttatgca agtgatcgag cagcgtacac agatcatcgg ccaactgatc
     3541 gtcgatcgat tcaacttgcg cgggtggagc agacagcggc agcggctcaa gcggcggcgc
     3601 agaaactttc agtaacggca gcagcgggac aaattcttgc ggcgcgaaat aaacttcacg
     3661 catggcacca ttgggcagct gctcaaagcc gcgatagatg aagccgagat accacagacc
     3721 ttcggcggga ctgaccggac tgcgccacgg ctgctcgcgt tccaatcgcg ccggaccaac
     3781 gggtcggatc gatccgaagc gctgcgcaaa attgatcgcg ggcaatgtgc cggcggcggc
     3841 gagcagcgat ctgaatgcat cccgttcggc gggcgagagc acttccatca ttttgctggc
     3901 acgggctggc tggcttaatt cgctttcgac acgtgcgacg agatcgcgcg gctttaaccc
     3961 cgttgcatcg atcgaccaac gtgaagcgat cacgcgcagc agagcggcat cggtatccag
     4021 cagacattgg cgcagcgttc gcatgcgtta attataaatg acaatggcac agatgtataa
     4081 tatcaatgtg gtttgtcgaa taatgttgta tcgtgagcaa gaaaacaagg taatggctga
     4141 tgatcaacag aggcaggaag ttcatttcct ttggatcgag tttgctagtt gtcggaattt
     4201 atgcattttg cagctgcgcc ttcaaccttt tcctcaattg gtatgatact ggcgcgatta
     4261 atagtgaagg ctggttgcca accgttatcg gcggcgctgt cttcggtttg atcgcagtca
     4321 gtatcgagcg gaggatctat tatcaacggc aggagctgtg gcctcaaaag cgtttactgt
     4381 tttatgtgcg actcatcata ctgagcattt tctacgttga tctcggcttg gggtttggtt
     4441 ctggtttcga ttatatgctc agggaaatct ttgctggcac aacatttttt gtcgctctta
     4501 aggatttatt actcgtcccc gcaattgtag tcttttactg gttaatgatc ttgcccttcg
     4561 gcttgactgc gggtctgtta atggggttac tgttgcgaag caatctttct gctcaatcat
     4621 tccgtgaaga tcaagtcgat tacatcgaat cgttaggtta gctgatcttc tggtcatcta
     4681 tactcgccta atcaatgctc ataggagttt catgcccgcc cattcagaat tgttgtttca
     4741 tctcacacgc ccgccgctgc aaaaagattc ggctgcgccg ccgctgttgt tgctgctgca
     4801 tggcgtgggc agcaacgaag aagatttatt tgaactgagc aaatatcttg atccgcgatt
     4861 tttcatcgtc agtgcccgcg cgccgattca gttgatgccc ggcgcatacg cgtggtatca
     4921 cgttcagttt ttgccggatg atatgcggat cgatttccgc gaagtggagc acagtcgatc
     4981 gatcttgcag cgtttcatcg ccgagttgat cgagtcgtat caggttgatc ctgatcgtgt
     5041 ttacttgatg ggctttagcc aaggtgcgat tatgagcttg agtttagcgt tgactgaacc
     5101 cgatcggctg gcggccattg tcgcgatgag cggacgcttg ctgccggaat tgaccccgca
     5161 aattgcggcc cccgaaatgt tgaaagactt cccgatattc atggcgcacg gtcttgccga
     5221 taatgtgata ccgatccgcg atgcacgggc ggcacacgaa caattatcca agctgcctgt
     5281 cgatctcact tatcgcgaat acccgatggg tcatcaagtc tcgatcgaga gtttgaatga
     5341 catcgccgct tggctgaaag atcaactcga tcgaaaacct gcctcaaaag cttgacattc
     5401 tttgatttca gcgtaagctc cccgctcgat ttgccttcca ttcgatcgat ccgctgttga
     5461 ggccctatga tcaattcacg cttgttatcc atcatgcgca aagagatcat ccagatcacg
     5521 cgcgatccgc gcacgctagt gatctcgttc atgatgccga tcttgatgtt gttcctgctc
     5581 ggctttgcgg cgacgagcga tctgcgcaac gtgccggcgg tgctgttcga tcaagatcac
     5641 tccaaagcgg cgcgcgatct gctggacgct tatcgggccg ccgattattt tagtttcgac
     5701 tttgaagtgg attccgcgca agccctgcgc cagttgatcg atagcgggca ggccaaagcc
     5761 ggcttgatca ttccgcccga ttacgggcag cacattgcca gcggtgaaac ggcctcggtc
     5821 gcgttcgtga tcgatggttc tgatccgacg gtggccagca ctgcgttgtc ggccgctatg
     5881 ctgatcgggc aaacgaaatc cacggcggtc ttgcgcgcca cactcgacgc gcgcggccaa
     5941 tccaatgcgg ccggttcgcc gatcgaagtt cggacgcagg tttggtataa tcccgatctg
     6001 gtcagcgctt actttatgat tcccggcttg atgggcacga tcttgttgca aatggctgtg
     6061 ctgttgacct cgtctgcgat cgtgcgcgaa cgcgagtacg gcaccatcga gcagttgatc
     6121 atcacgccca ttcgatcgtg ggaattgatc atcggcaagc tcacgccgta cgtgtttatc
     6181 gcgtttttcg atttgctgga agtgctggtc ctgggcacgt tggtcttcaa agtgccgatg
     6241 gccggcaatg tcggtttgct gctgctgatg tcggctttgt ttctggcgac ggcgctcggc
     6301 atcggcttgt tcatctcgac ggtcgccaaa acgcagcagg aagcgctcat gctgggcgcg
     6361 cttattcaac tcccctcgtt ttttctcgcg ggcttctttt tcccgatcgc cgcgatgcca
     6421 cccttcttgc aggcgatcag ctatctcatc ccactgcgtt attacctgac gattgtgcgt
     6481 gcgattatcc tgaagggcgt gggcattgaa tccatcgccg gcgacgtgat tcccttggct
     6541 attttcggcg tgctgattgt caccgcggcg gtgctgcggg ttcgcaaacg attagactga
     6601 gttttttgga gtgccgactt cagtccgcgc tccactgatt tcgaattttt ggaggaagca
     6661 tgcataagcg attgcgtt
//
LOCUS       DASRTJ010000385         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_61752,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000385 DASRTJ010000000
VERSION     DASRTJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61752"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFF70_07750"
     CDS             complement(<1..1350)
                     /locus_tag="VFF70_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44627.1"
                     /translation="MASTIIRRWSISLTLSLLVLAAIGFSLSRSTIVNASGGNPYPGV
                     FPCNTTLQACINGLSDGDEIDIAGGTYTESFTLDKAISLVGDDSSTTFLVAAANNRVM
                     TVTGSSITSSVVISGLTFMGGNLSVGNGGGLLITATYPVALRNVVITGNTAQRGGGLY
                     LANSHVVAAGSTIAGNTADFGAGVYQKNGEFDLVSGAIEENNANFTGGGVYLWQATAE
                     FTQTGGSITTNTASYGAGVGINQGRFNLINGHIDQNDAASEGGAVFMDDPIGVFNQSG
                     GTLGWNSAAYGGGAKVINGTYNLSGGEIVGNQASSKGGGIYVYQVDAVFNQTNGSLEQ
                     NSAYRGGGVYLDSGTAALNGGLILSNTATDRGGGVYASGAITIGQIWLANNTATSSGG
                     GVFAANGLVISGTTFSDNTTFSGDGGGVLADVLLATNSSFTHNRSLTGYGGGVYAFYL
                     "
     gene            complement(1549..3168)
                     /locus_tag="VFF70_07755"
     CDS             complement(1549..3168)
                     /locus_tag="VFF70_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HZY44628.1"
                     /translation="MRTLEWDLKHRWKIEPAEEAQLLRLIRAASLLEAGRPKDMARLL
                     RKYPRASGQFSKDQLVAAYKQFCDQGHFTFDAEVLRRLQMKPTRTISGVATVTVLTKP
                     FPCPGECIFCPTDVRMPKSYLADEPGAIRAEYHHFDPYETALSRLRALENIGHTTDKI
                     ELLILGGTWSYYPVDYQAWFIQRCLDALNGVDSRSLEEAQQINETAEQRNVGLVIETR
                     PDYIDAEEVKRLRRYGVTKVQLGAQSLDDRILNLNKRGHSVEQTRQAVTLLRAAGFKI
                     HLHWMPNLLGGTPESDRIDYDRLWSDPGLRPDELKIYPNALVREAELIQWWERGEFRP
                     YTDEELIELIAGCKVRTPRYARLSRIIRDIPKGHIVAGSTVSNLREVVKRRLLQEGRA
                     CQCIRCREVRGARSDSHGSIGAIEYETTGGREIFLSVDTETDRLAGFLRLSLPTPEAI
                     DLIKIDELRGAAIIREVHVYGPVVDIGAQARSGHIQHSGIGTALIQEAEQRARSAGYT
                     TLAVISAIGTREYYRKHGFTSGELYLLKNLT"
     gene            complement(3262..3942)
                     /locus_tag="VFF70_07760"
     CDS             complement(3262..3942)
                     /locus_tag="VFF70_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44629.1"
                     /translation="MKLSVVIPVYDEVNTIQEILAQVRAVKLADEIIIIDDGSTDGTR
                     EKLKAEESQAGTVVIYQARNQGKGAAVRAGIDRATGDIILIQDADLEYDPRDYPNLIR
                     PIQEGRVKVVYGSRFLGPRTAMNFWNMIANKSLTLMTNLLYNAILSDMETGYKAFRAD
                     VIKGIPLRSKRFEVEPEITAKVLKRGHRIFEVPISYYGREVHEGKKIRWYEAPKAVWV
                     LLKYRFVD"
     gene            4040..4111
                     /locus_tag="VFF70_07765"
     tRNA            4040..4111
                     /locus_tag="VFF70_07765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4072..4074,aa:Gly,seq:ccc)
     gene            complement(4153..>4273)
                     /locus_tag="VFF70_07770"
     CDS             complement(4153..>4273)
                     /locus_tag="VFF70_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZY44630.1"
                     /translation="VFDGKFKPVIDQTFALKDARAAHERLERGENFGKIILVP"
BASE COUNT          963 a         1254 c         1079 g          977 t
ORIGIN      
        1 cagataaaag gcgtagacac cgccgccata accagtcagg cttcggttgt gcgtgaagga
       61 actgttggtg gccaagagca catcggccag tacgccaccg ccgtcgccgc taaaggtcgt
      121 gttgtcgctg aaggtcgtac cgctgatgac caacccgttg gccgcaaaca cgccgccacc
      181 actagacgta gctgtgttat ttgccagcca gatctgccca atcgtaatcg caccactggc
      241 atatacgccg ccgcctctat ctgtagcggt gttgctcaag atcaatccgc cgttcagcgc
      301 cgccgtcccg gagtccaagt acactccgcc gccccggtag gctgagttct gctcgagcga
      361 accgttggtt tgattgaaga cggcatcgac ttggtacacg taaatgccgc cgcctttgct
      421 ggatgcttga ttgccgacga tttctccgcc gctcaagtta tatgtgccgt tgatgacctt
      481 cgccccgccg ccatacgccg ccgagttcca gcccagcgtg ccgcctgatt ggttgaacac
      541 gccgatcgga tcatccatga acaccgcacc gccttcactc gcggcatcgt tctgatcaat
      601 gtgaccattg atcagattga aacggccctg attgatcccg acgcctgcgc cgtacgaagc
      661 agtgttcgtc gtgatcgatc cgccggtctg ggtgaactcg gcggtcgctt gccaaagata
      721 cacaccgcca ccagtaaagt tggcattgtt ctcctcgatg gcaccactca ccagatcgaa
      781 ctcgccgttc ttttgataca caccagcgcc aaaatccgcg gtgttaccgg cgatggtgct
      841 gccagcggcc actacatgac tgttcgccag atacaggccg ccgccgcgct gtgcagtgtt
      901 gccagtgatc acgacattcc gcagcgcgac gggatatgtt gccgtgatca acaatccgcc
      961 gccattgccc acgctcagat ttccgcccat aaaggtcagg ccggaaatca ccacgctgct
     1021 ggtgatcgaa gagccggtca cggtcattac ccgattgttc gccgcagcga ccaagaacgt
     1081 cgtagacgaa tcatcaccca ccaagctaat cgccttatca agcgtaaaac tctcggtata
     1141 agtgcccccg gcaatatcga tctcgtcgcc atcactcaga ccattgatgc aagcttgcag
     1201 tgtcgtgtta cacggaaaca ctccgggata ggggttcccg ccactagcgt tcacaatggt
     1261 cgatcgacta agcgaaaaac caatcgccgc caaaacaagt agagataatg taagtgaaat
     1321 agaccaacgt cggataattg tggatgccat actgaattcc tcctctacag atgcgtggaa
     1381 atgcgcagca ccagctgctc aagctcaatg aagaatccgg ggttaagttg tatgaaacgg
     1441 ccagccctgg cgactttaag ttgcagtttt gctttcattc taacagagaa tctgtcagaa
     1501 ctcaccatga cctttaggct acttgccacc tgcaaccttt aacttaactc atgtcaagtt
     1561 cttcagtaaa tacagttcgc ccgaggtaaa tccgtgcttg cgatagtatt cgcgtgtgcc
     1621 gatggcggag ataaccgcca gagtggtgta acctgccgag cgggcacgct gttcagcctc
     1681 ttgaatcaac gccgtgccaa tgcctgaatg ctgtatgtga cccgatcggg cttgagcgcc
     1741 aatatcaact accggcccat acacatgcac ttcccgaatt atcgcggcgc cgcgcagttc
     1801 gtcgatcttg atcagatcga tcgcctccgg cgtgggcagc gataaacgca gaaaacccgc
     1861 taaccgatca gtttccgtat caacactcag aaaaatttcg cgcccgccgg tcgtctcgta
     1921 ctcgatcgct ccgatcgagc catggctatc cgatcgagca ccgcgcactt cgcgacagcg
     1981 aatgcactgg caggcgcggc cttcttgcaa tagtcgccgc ttaacaacct cgcgcagatt
     2041 ggaaacagtg ctgcccgcca caatatgccc tttgggaatg tcgcggatga tgcggctaag
     2101 ccgcgcgtaa cgcggcgtgc gtactttgca cccggcaatc aactcgatca attcttcatc
     2161 ggtgtacgga cggaactcgc cgcgctccca ccattgaatc agttcggctt cgcggactag
     2221 cgcgttcgga taaatcttca attcatcggg gcgtaaaccg ggatcactcc acaatcgatc
     2281 gtaatcgatc cgatcgctct caggcgtccc gccgagcaag ttcggcatcc aatgcaagtg
     2341 gattttgaaa ccagccgcgc gcaataacgt caccgcttgt cgagtttgct cgacagagtg
     2401 accgcgctta tttagattga gaatgcgatc gtccagactt tgcgcgccga gttgcacttt
     2461 ggtcacacca taccgtcgca gccgcttcac ttcttcagca tcaatgtaat caggccgcgt
     2521 ttcgatcacc aggccaacat tgcgctgctc ggcggtctca ttgatctgtt gggcttcttc
     2581 caacgatcga gagtcaacac cattgagcgc atcgaggcag cgctgaatga accatgcctg
     2641 ataatcgacc gggtagtacg accaggtgcc gcccaagatc aacaattcga tcttgtcggt
     2701 ggtgtggccg atgttttcta aagcgcggag gcggctgagc gcggtctcat acggatcgaa
     2761 gtgatgatat tcagcgcgaa tcgcgcccgg ctcatcggcc agataactct tgggcatgcg
     2821 cacatcggtc gggcaaaaga tgcactcgcc gggacacggg aacggcttgg tcagcaccgt
     2881 caccgtcgcc acaccggaga tcgtgcgtgt gggtttcatc tgcaagcggc gcagcacttc
     2941 ggcatcgaaa gtgaagtggc cttgatcgca aaattgtttg taagcggcga cgagttgatc
     3001 tttggagaat tgtccgctgg cgcgaggata tttgcgcagc agacgcgcca tgtcttttgg
     3061 gcggcccgct tcgagcaatg acgcggcacg aatcagccgc agcagctgcg cttcctcagc
     3121 cggctcgatt ttccatcgat gtttgaggtc ccattcgaga gtgcgcatgt aatcctaata
     3181 acttattaac gtgataacgc gaagtgtgac aagtggcgag tgatcaatcg ataaacgcta
     3241 ggacataggc gctaagcgtt ttcaatccac aaaccggtac ttcagcagca cccagaccgc
     3301 cttcggcgct tcataccagc gaatcttctt cccttcatga acttcgcggc cataatagga
     3361 gatcggcact tcgaaaatgc gatgaccccg ctttaaaacc ttggccgtga tctcgggctc
     3421 cacttcgaac cgtttagagc gcagtggaat gcctttgatc acgtcggccc tgaaagcttt
     3481 gtacccggtt tccatatcgc tcaagatcgc gttatacaaa agattcgtca ttagtgtcag
     3541 cgatttattt gcgatcatat tccagaagtt catcgccgtg cgcggaccga gaaagcggct
     3601 gccatatacc actttgacgc ggccttcttg aatcggccgg atcaaattcg gataatcgcg
     3661 cggatcatat tccagatcgg cgtcttgaat caggatgatg tcgcccgttg ctcgatcaat
     3721 tccggcgcgt acggccgcgc ctttgccctg attgcgtgcc tgatagatga cgactgtgcc
     3781 cgcttggctt tcctcagcct tcagcttctc gcgcgtaccg tcggtcgatc cgtcgtcgat
     3841 gatgatgatc tcatcggcca gcttcactgc acgcacttgc gccaggattt cttgaatcgt
     3901 attgacttcg tcataaactg gaattaccac agatagtttc atcgactgct ctttcgtcgg
     3961 cgagattgaa tccatattct accagaataa cccggcgata cttgacacga cttcaagccc
     4021 ggctataatc catgcacacg cgggtgtagc gcagtggtag cgcaagtgct tcccaagcac
     4081 tgggccgtgg gttcgaatcc catcacccgc tcacaaaaac tcgaagggtt tgagaaaccc
     4141 ttcgagttat ttttatggaa ccaaaataat ctttccgaag ttctcgcccc gctccaggcg
     4201 ttcgtgcgcc gcacgtgcat cttttaaggc aaacgtctga tcgatcaccg gcttgaactt
     4261 tccgtcaaat acc
//
LOCUS       DASRTJ010000386         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_61815,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000386 DASRTJ010000000
VERSION     DASRTJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61815"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFF70_07775"
     CDS             complement(<1..308)
                     /locus_tag="VFF70_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44631.1"
                     /translation="MIEPPASRSEVWRTRFRRARARLRKVLPFASGVFAAFLALLLYN
                     LVVPGQRPLTTRDVNNTITQYLATATPPPAYSERVYQIIRPSLVLIETEGLDSTGKSE
                     G"
     gene            562..1611
                     /locus_tag="VFF70_07780"
     CDS             562..1611
                     /locus_tag="VFF70_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZY44632.1"
                     /translation="MIHSLPPFTTTLIGSFPHLDADALCRSLGASIDIPAWPQLPRRN
                     FRESMYVQYSARLPGVQLDEVNQKITFNTIDDLSPALEIFYEHVLADDVNWFALEPDY
                     AAGFFALLNAAKNFSGEWLKGHVTGPISFGLTVTDQSLRSSLYNEALADVIVKNMALN
                     TRWQIRQLQSVRDHVIIFVDEPYLAAFGSAFISVSREQVIALLDEVFEALHLEGALAG
                     VHCCANTDWSVLLATQVDILNLDAYGYLENLALYPIDLRNFLDRGGRIAWGIVPNNEG
                     ILNVDADRLADRMRAGLNLISTKARSRGVEIRSAELADRSLITPSCGLGSTSIEIADR
                     VVETLARTGEILKRG"
     gene            complement(1638..2429)
                     /locus_tag="VFF70_07785"
     CDS             complement(1638..2429)
                     /locus_tag="VFF70_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY44633.1"
                     /translation="MTTTIALAGKGGVGKTTIAAMIIKYLIQSQNGAVLAIDADPSSN
                     LNMVLGLDLEWTVGDIREDLLGQVKQSLTAGGAAMGTLPGGTNKRDYLDYHIRSSLME
                     GSRFDLIAMGRGEGQGCYCAVNHNLREVIDAMSKSYQYVVIDNEAGMEHLSRRTTRDV
                     QHLLIVTDPTQRGIVAAERIASFRNELDIDIENTYLILNDLTAGEVPAPLQSRIDQLD
                     FPLVGTVPHDDGAKEFEFSGRPLIELNDETPLYRSVAALMQKILA"
     gene            complement(2434..>3102)
                     /locus_tag="VFF70_07790"
     CDS             complement(2434..>3102)
                     /locus_tag="VFF70_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44634.1"
                     /translation="PCLAGALGFPPDLRLKLHPIAEMDQLIEERTGAKPGTVGGFFTI
                     NPRVDDIPDRFSIAHRNVRLLEMGSVDIGGTGCICPESALLKTLFTHLLFRQDDVLIL
                     DMYAGVEHLGRATVDFVDAMIIVVEPTRRSLGTAGQIKKLAKDIGLTRLWLVGNKVRN
                     QDEVDFLKTETPDIPVLGFLEANLVVQEADRLGIAVYDHVPALRRSAEEMAHLLSEQI
                     KERA"
BASE COUNT          702 a          863 c          827 g          710 t
ORIGIN      
        1 ccttccgatt tgcccgtcga gtccagacct tcggtttcga tcagcaccaa cgaaggccgg
       61 atgatttgat agacgcgttc cgagtaagcc gggggcggcg tggccgtggc cagatattga
      121 gtaatcgtgt tattaacatc gcgcgtggtg agaggtcgct ggccaggcac aaccagattg
      181 tagagcagca aagcgaggaa cgcggcgaac acgccggagg caaacggcag gactttacgc
      241 aggcgagcgc gagcgcgtcg aaaacgagtg cgccacactt ccgatcgaga agcaggaggt
      301 tcgatcatct tttaaataag cgaatcatac ttaaggataa acggtgatac ggctgttggc
      361 tgccaatgga tttattatac ggcgttttga tgaattagag atgagaaaat gctgagggcg
      421 ttcgtgagaa aatgcggccg ccggtcgcta ttttgtgaaa acaaaaaaac acgatcgtca
      481 attcgattct caccattcta cactgaaaaa tcatgaagaa tatcactcgc caattatcag
      541 cccgggtttt acactgaacc catgatccac agcctgcctc cctttaccac caccctcatc
      601 ggcagttttc cacatctcga tgcggacgcc ttgtgtcgat cgctcggcgc ctcgatcgat
      661 attccagcct ggccgcaact gccgcgccgg aattttcgcg agagcatgta tgtgcagtac
      721 agcgcgcgct tgcccggcgt ccaattggat gaagtgaatc agaagatcac cttcaacacg
      781 atcgatgatc tcagtcccgc gctggaaata ttctacgagc atgtcctggc cgacgatgtg
      841 aattggttcg cacttgaacc cgattatgcc gccggctttt tcgcgctgct gaatgcggcc
      901 aaaaactttt caggcgaatg gctgaaaggc cacgttaccg gcccgatcag tttcggactc
      961 accgttaccg atcaaagtct gcgatccagt ttgtacaacg aagcattggc cgacgtgatt
     1021 gtcaagaaca tggcgcttaa cacgcgctgg caaattcgcc aactccaatc ggtgcgcgac
     1081 cacgtcatca tctttgtcga tgagccgtat ctcgcagcgt ttggctcggc ctttatcagt
     1141 gtgagccgcg agcaagtcat cgccctgctc gacgaagtct tcgaagcgct tcatctcgaa
     1201 ggcgcgctgg ctggtgtgca ttgctgcgcg aataccgatt ggtcggtcct gctggcgacg
     1261 caggtcgata ttctcaatct cgatgcgtac ggttatttgg agaatctggc gttgtaccca
     1321 atcgatctgc gtaacttttt agatcgaggc ggacgcatcg catggggcat cgtgccgaac
     1381 aacgaaggaa tactcaacgt cgatgcggat cgcttggccg atcggatgcg ggcggggctg
     1441 aatttgatca gcaccaaagc ccgatcgcgc ggagttgaga tccgatcggc tgaactggcc
     1501 gatcgaagtt tgatcacacc gagttgcggc ttgggatcaa cctcgatcga gattgcggat
     1561 cgcgtagtgg aaacattggc tcgaactggc gagattctga aacgaggata aattgtaggg
     1621 gcgaggtgac ctcgccctta cgctaagatt ttctgcatca gcgccgcgac cgatcgatac
     1681 agcggtgtct catcattcaa ctcaatcagt ggtcgaccgc tgaattcaaa ttctttcgcg
     1741 ccgtcatcgt gcggcaccgt gcccaccaac ggaaagtcca gttgatcgat ccggctttgc
     1801 agcggggccg gaacttcgcc cgccgttagg tcattcaaga tcagatacgt gttttcaata
     1861 tcgatgtcga gttcgttgcg aaacgaggcg atgcgttccg ccgcgacgat gccgcgctgc
     1921 gtcggatcgg tgacaatcaa cagatgctgc acatcgcgcg tggtgcggcg gctcaagtgc
     1981 tccatgcccg cttcgttatc gatgacgaca tattgataac tcttgctcat cgcatcgatc
     2041 acttcgcgca ggttgtgatt gaccgcgcag taacagcctt gaccctcacc gcgccccatc
     2101 gcgatcagat cgaagcgact gccttccatc aacgacgatc gaatgtgata gtctaagtaa
     2161 tcgcgcttgt tggtgccgcc cggcagcgtg cccatcgccg caccgcccgc cgtcaatgat
     2221 tgcttgacct gccccaataa atcttcgcgg atgtcgccga ccgtccattc cagatcgagg
     2281 ccaagcacca tgttgaggtt gctgctggga tcagcatcga tcgccaggac agctccgttt
     2341 tgcgattgga tcaaatactt gataatcatc gccgcgatgg tcgtcttgcc tactccgcct
     2401 ttgccggcca atgcaattgt cgtcgtcatt gtcttacgct cgttctttga tttgctctga
     2461 caataaatgc gccatctctt ccgccgatcg acgcagcgcc ggcacatgat catacacagc
     2521 gatgcccaat cgatccgctt cttgcacgac gaggttcgct tcaagaaaac ccagcacggg
     2581 aatatccggc gtttcggttt tgagaaaatc aacttcgtct tgattgcgaa ctttattgcc
     2641 aacaagccac aagcgggtca agccaatatc cttcgctaat tttttgatct gcccggctgt
     2701 gcccaaactc cggcgtgttg gttcaaccac gatgatcatt gcatcaacaa agtcgacggt
     2761 cgcgcggcca agatgttcga cgcctgcata catatccagg atcaacacat cgtcctggcg
     2821 gaagagcaag tgagtaaaca aagttttcag cagcgcgctc tcgggacaga tgcagcctgt
     2881 tccgccgatg tcgaccgatc ccatttcgag caagcgtaca ttgcggtggg cgatgctgaa
     2941 tcgatcgggg atgtcgtcaa cacgcggatt gatcgtaaag aagccgccga cggtgccggg
     3001 cttcgcgccc gtccgttcct cgattaattg atccatctcc gcgatcgggt gcaacttcaa
     3061 tcggaggtca ggcggaaaac ccaacgcgcc cgccagacac gg
//
LOCUS       DASRTJ010000387         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_23840,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000387 DASRTJ010000000
VERSION     DASRTJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23840"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFF70_07795"
     CDS             complement(<1..1012)
                     /locus_tag="VFF70_07795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZY44635.1"
                     /translation="MAETQNIISMTGIAKSFPGVIALEDVDFSLRKGEIHALMGENGA
                     GKSTLIKVLTGVEHPDKGSIEIEGKAVQVRSPQHSQELGISTVYQEVNLCTNISVAEN
                     IMLGHESQRFGSVNWRKMNAQAKQAIQRLDLELDVTRPLGDYSVAIQQMVAITRALEI
                     SSAKALILDEPTSSLDIHETNLLFEVMRKLKSKGLGIVFITHFLDQVYQIADRITVLR
                     NGKLVGTFDTVSLPRVELVAKMLGRIVTDLEAMSKAKFKSEQQRAKKNLVEAKGLGLK
                     GSLEPVDLELKTSEVVGIAGLLGSGRTEMADLIFGIDTPDTGSLLVNSQAINRFSPLD
                     SLK"
     gene            complement(1218..2228)
                     /locus_tag="VFF70_07800"
     CDS             complement(1218..2228)
                     /locus_tag="VFF70_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY44636.1"
                     /translation="MKTSKVSFFVVMLVVLSVLAGCAQAATPAPAPVAPAGPTAAPAK
                     TTYTYKDLTLCYPQLGAESDWRTANSASFKETAAQLGIKQLIFSDAQQKQENQISAMR
                     ACIQQAVNVIALPPVVETGWDTVLTEAKNAGIPVIIVDRSVTADASLYAAHIGSDMVL
                     EGERSAAEFNKLLPNGGNILELSGTTGSGAAVGRAKGFRAKLNANIKILDSQTGNFTR
                     AEGKPVMEAFLKKYAGQIQGVFFHNDDMAIGAVEAIKAAGLKPGDLKMISIDGTRGGF
                     QLMIDGWLQADVECNPLLGPQVMDLAVKLMNGEAITKEVLTDESTYYPDKAAELLPTR
                     KY"
BASE COUNT          588 a          678 c          696 g          627 t
ORIGIN      
        1 gtttaaggga atccaagggc gaaaaacgat taatcgcttg actattgacc agcaatgaac
       61 cggtatcagg cgtatcgatg ccaaagatca ggtcggccat ttcagttcgg ccagaaccca
      121 acaagccggc gatcccaacc acctcgctgg tcttcaactc cagatcgacc ggttcaagcg
      181 agcctttgag gcccagcccc ttggcttcga ccagattctt tttagcgcgc tgttgttcgc
      241 tcttgaattt ggctttggac atcgcttcca gatcggtcac aatgcggcct aacattttgg
      301 cgacgagttc gactcgcggc agggaaacgg tatcaaacgt gccgacgagt ttaccgttgc
      361 ggagcaccgt gatccgatcg gcgatctggt aaacctgatc cagaaaatgg gtaataaaaa
      421 caatgccaag ccctttactc tttaattttc gcatcacctc aaagagcaga ttggtctcat
      481 ggatgtccaa actggacgtg ggttcatcca ggataagtgc tttcgccgag gaaatttcaa
      541 gggcgcgcgt aatggccacc atctgttgaa tggccaccga ataatcgccc agcggccggg
      601 tcacgtccag ctcaaggtct aggcgctgga tggcctgctt ggcctgagca ttcatctttc
      661 gccagttcac gctgccgaaa cgctgagact catggcccag catgatattt tcagcgacag
      721 atatattggt gcacaggtta acttcctgat aaaccgtgct aatgccaagc tcctgagaat
      781 gctgcggcga tcgcacttga acggctttac cttcaatctc gatggagcct ttatcaggat
      841 gctccacgcc ggtcaggact ttaattaagg tggattttcc cgctccgttc tcgcccatca
      901 aggcatgaat ctcacctttt ctcagtgaga agtcaacatc ttcaagggcg atgacgcccg
      961 gaaaggattt ggcgatgcct gtcatgctga tgatattctg agtctctgcc atagaaatga
     1021 cctgccttca acattgatcg cattcacggg ctgaatgcgg tgctgcctgg cgcgggaata
     1081 aaagaggctg cgtgaataat ttttattata acatcaggtg caagtgcgag agaagcggcg
     1141 ttgagttcta acgccgcttc tctatatctc attcgatcat gcgcacgatt ccgggaaatc
     1201 ttacgcggcc tgttgattta gtacttgcgg gtcggcagta actcggcagc cttatcagga
     1261 tagtaggtac tctcgtcggt gagaacttcc ttggtaatgg cttcaccatt catcagtttc
     1321 acagccaagt ccataacctg cgggccaagc agcggattgc attcaacgtc cgcttgcagc
     1381 cagccgtcga tcatgagctg gaagccgccg cgggtaccgt cgatggaaat catcttgaga
     1441 tcgcctggct tcaaaccagc cgccttgatg gcttcgacag cgccgatggc catgtcgtca
     1501 ttatggaaga acacgccttg aatctggccg gcgtatttct tcaggaaggc ttccatgacg
     1561 ggcttgcctt cagcacgggt gaagttaccg gtctgggaat ccaagatctt gatattggca
     1621 ttcagcttcg cgcggaagcc tttggcgcgg ccgactgcag cgccggagcc agtggtgcca
     1681 gacagctcaa ggatgttgcc gccgttgggg agcagcttat taaattcggc ggccgatcgt
     1741 tcaccttcga gcaccatgtc cgatccaatg tgcgcggcat acagggaagc gtcggcggtg
     1801 accgatcgat ccacaataat gaccggaatg ccggcgttct tggcttcggt caagactgtg
     1861 tcccaaccgg tttcaaccac ggggggcagc gcgatgacat tcacggcctg ctgaatgcag
     1921 gcgcgcatcg ccgagatctg gttttcctgc ttctgctggg catcagagaa tattaactgc
     1981 ttgataccca actgggcggc agtctctttg aatgaagcag agttggccgt gcgccagtca
     2041 ctttcggcac cgagctgagg atagcacaac gtcaggtcct tgtacgtata ggtggtcttc
     2101 gcgggcgcgg cggtcggtcc agcgggcgcg acgggcgctg gggccggtgt agccgcctgt
     2161 gcgcaaccgg cgagcacgga gaggacgacc aacattacga cgaaaaacga aactttcgag
     2221 gtcttcattg aattctcctt ctgggtattt ttctggatag ggtcgttcgt cgatacgcat
     2281 ttccccccgg aggaaatgct atgatccttc ccctctaatc tataacctag tcgaatgaga
     2341 gtcaatgagt tcacattagg aatgagtgga gatttacact ttgttgcctt accggaaaca
     2401 caggtagcta ggcgcaaggt ggatttcttt tgatagcagt ggaatttccc cattgagcca
     2461 tgatccaccc ctcgttggat tgactgaagc agcaatttaa aacaaagcga tgcctgcctt
     2521 tcgccgtgtg tcgattcatt tggttgagga gatcaatcga gcgacgcaag caagccgaat
     2581 cctaccggt
//
LOCUS       DASRTJ010000388         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_350829,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000388 DASRTJ010000000
VERSION     DASRTJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350829"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1679)
                     /locus_tag="VFF70_07805"
     CDS             complement(<1..>1679)
                     /locus_tag="VFF70_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZY44637.1"
                     /translation="THAGPGQPFLEVFIMEPAHAQILISTQPNEEGKFREDLPYFTEG
                     RRAPYIQSIYYSFALQGAAMTAAAPLRAANGELLGVLAARLNLEEMNAVINRRNGLHA
                     TDDFFLVTTANLFATQPRFISDPAVLQRGVYTEQVNRCLAGNSGVLAGEDYRGEPALV
                     VYRWLDDLQLCLITKLDQSEAFAPVRDLTITLIIVAVLVLGAASALSFSISRTLTRPL
                     IVLHTNAVKLGAGDLTVRVSETTGDEIGLLAREFNTMAAAILDKETQLRNYTADLEQR
                     VADRTVELQTSEASFRYLFANNPHPMWVYDLETLAFLEVNDTAVDRYGYTRSEFLHMK
                     ITDIRPPEDVARLVADVQPKDRPAIERSDNWRHRLKDGRIIDVEILSHTLEFGGLHAV
                     LVVAHDITDRKRAEATRDQLAAIVASSEDAIIGETLDGTIASWNKGAEKVYGYPREDV
                     VGQPISILIPTDLPNELSAILVRLNMGESIEGYETTRVRKEGQRINVALTISPIRDST
                     GNITGASTIARNITDRKQAEEALLASEARYRHTLNAMLEGCQIIGFDWRYL"
BASE COUNT          371 a          486 c          447 g          375 t
ORIGIN      
        1 gaggtaccgc caatcaaatc cgatgatttg acagccttcg agcatggcgt ttaacgtgtg
       61 acgataccga gcctcactgg cgagcaacgc ttcttccgcc tgcttgcgat cggtaatgtt
      121 gcgggcgatt gttgaagcac cggtgatatt tcccgtcgaa tctctaatcg gtgagatggt
      181 taaagcgaca ttgatacgtt gaccctcttt tcgaacgcgc gtcgtctcat agccttcgat
      241 actttcgccc atgttcaacc gaacgagaat tgccgacaat tcatttggca gatcggttgg
      301 aattaggatc gaaatcggtt gccccaccac atcttcacgg ggatacccat agactttctc
      361 ggcgccttta ttccagctcg caatagtacc gtccagtgtt tcaccaatga tcgcatcttc
      421 cgacgatgcc acaatcgccg ccaattgatc acgtgtcgct tccgcccgct tccgatcagt
      481 gatgtcatgc gccacgacca aaaccgcgtg caggccgcca aactccagcg tgtgcgataa
      541 aatctcaacg tcgatgatcc gcccgtcttt caaacgatgc cgccaattgt ccgaccgctc
      601 aatcgcgggc cgatcttttg gctgcacatc ggccaccagc cgcgccacat cctccggcgg
      661 tcgaatgtcg gtgatcttca tgtgcaaaaa ctctgagcgt gtatagccgt agcgatcaac
      721 cgccgtgtca ttgacttcca gaaaggcgag cgtctccaag tcgtacaccc acatgggatg
      781 tggattgttg gcaaacaaat agcggaagct ggcttcgctg gtctgcagtt ccaccgttcg
      841 atcggcgacg cgctgttcca agtccgccgt gtaattgcgc aattgagttt ctttatctaa
      901 aatagccgcg gccatcgtat tgaattcgcg tgctagcaga ccgatttcat cgcctgtagt
      961 ttctgacaca cgcacggtga gatcgcctgc gcccaatttc actgcgttgg tgtgcaggac
     1021 gatgagcggg cgcgtaaggg tacgtgatat agaaaacgaa agcgccgacg ccgcccccag
     1081 caccaacacc gccacgatga tcaatgtgat ggtgagatcg cgcaccggcg cgaatgcttc
     1141 ggattgatcg agtttggtga tgaggcacag ctgcaagtca tcgagccagc gatagacgac
     1201 aagggccggc tcaccgcgat aatcttcccc cgccaggaca ccgctattgc ccgccagaca
     1261 gcgattgact tgctcggtgt acacgccgcg ctgaagcacc gccggatcac tgataaagcg
     1321 cggctgagtc gcgaaaagat tcgccgtcgt caccagaaaa aaatcatcgg tcgcgtgcag
     1381 accgttgcgg cgattgatca cggcgttcat ctcttctaaa ttcaagcgcg ctgccagcac
     1441 gcccagcaat tcaccatttg ccgcacgcag cggcgcggcg gcggtcatcg ccgcaccttg
     1501 tagcgcaaac gagtagtaga tgctttgaat atagggcgca cgccgacctt cggtaaagta
     1561 cggcaggtct tcgcggaatt ttccctcttc attgggctga gtggagatga ggatttgggc
     1621 gtgggccggt tccatgatga acacttcgag aaaaggctgc ccgggaccgg cgtgcgtct
//
LOCUS       DASRTJ010000389         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_161738,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000389 DASRTJ010000000
VERSION     DASRTJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161738"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFF70_07810"
     CDS             <1..396
                     /locus_tag="VFF70_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44638.1"
                     /translation="PITPDQLRSLLSEVIERHALPSVPKRKPGAPKYRAEPNVHLLAA
                     KRAINNREFDEARGHLAEVIKQNDRMIEAHNLLGVLYEMKQEFAIAKRCYGRAIAIDS
                     KYEPAQQNMRRLFELFEFGTSKEPINLGE"
     gene            516..1787
                     /locus_tag="VFF70_07815"
     CDS             516..1787
                     /locus_tag="VFF70_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZY44639.1"
                     /translation="MLVYWARRQLRPAGMCIDTMSATRFVVLAIVSLGSRFAFAGETS
                     VPVETKPPESRFKISGWIDSGITFNPDSPQDNQNFGRYFDDRANEPLLNQAVINFERA
                     LAPQPGELDWGFKLQLMYGSDARFIHSLGLFSDTAATSIVQPDLVEAYLNLHFPVFTE
                     GGLDLKLGKFVTLEGAETIDPRTNFFYSHTYIFNFGIPFNHTGALATFHATKWLDVMG
                     GVTRGVNTSIDDNNDALAFHGGIGLNFNEGKVVVAASTHIGPETPNNDHDLRYLNAIT
                     TTWKINDKLTSITDLNYALDEVTDAKAYGIAQYFTYTINNWLTAGIRGEIFRDEQGFY
                     VVSFADTHDPIRVLAGEPAIDPRTVGGGRTTYGAVTAGVNIKPPMPKPLAGLTIRPEV
                     RYDRALTDTRPFNDSSQRDQFTIGVDFILAF"
BASE COUNT          483 a          594 c          536 g          462 t
ORIGIN      
        1 ccaatcacac cggaccaact gcgttccctc ttgagcgagg tcatcgagcg acacgcgctt
       61 ccttccgtgc cgaagcgaaa accgggtgcg ccgaagtaca gggccgagcc gaacgtgcat
      121 ctgctcgccg ccaagcgcgc cataaacaac cgggagttcg acgaagcccg cggtcatctg
      181 gcggaagtga tcaaacaaaa tgaccgcatg atcgaggccc acaatcttct cggcgtctta
      241 tacgaaatga agcaggaatt cgcgatcgcg aagcgctgct atggccgcgc cattgcaatt
      301 gactccaagt acgaacccgc gcagcaaaac atgcgacgcc tcttcgaatt atttgaattc
      361 ggcacgagca aagagccgat caatctcggc gagtagacgg aactcttgca atttgcagtt
      421 cggcaaaact gaaagcctgc gttttgcacg gaagccttca cggttccaag cacggccgat
      481 cacgctaact cgttcaagcg aaaggagata gtttcatgct tgtctattgg gcacgccggc
      541 agctaagacc tgctggcatg tgcatcgata caatgtctgc aacacgtttc gtggtcctcg
      601 cgatcgtgag ccttggttca cgtttcgcgt tcgcagggga aacaagtgtg ccagtggaaa
      661 cgaagcctcc agaatcgcgt ttcaagattt ctggctggat cgacagcggc atcacgttca
      721 atccggacag cccgcaagat aatcagaact tcggccgtta ttttgacgat cgcgcgaatg
      781 agccactcct aaaccaagcc gtgattaatt tcgagcgcgc gctggcgccg cagcccggcg
      841 agttagattg gggcttcaaa ttgcaactca tgtacggaag cgacgcgcgc ttcatccatt
      901 cgcttggcct gttcagcgat acggcggcta cgtcgatcgt ccagccggac ctggtcgagg
      961 cgtatctgaa tctgcacttt cctgtcttca cggaaggcgg ccttgatttg aaactgggca
     1021 agttcgttac cctggaagga gccgaaacga tcgatccgcg gacaaacttc ttctattcgc
     1081 acacttacat cttcaatttt ggaatcccgt tcaatcacac gggcgcgctg gcgacgtttc
     1141 acgcgaccaa gtggctcgac gtaatgggtg gcgtgacgcg tggcgttaac accagcatcg
     1201 acgacaacaa cgacgcgctg gcttttcacg gagggatcgg tttgaacttc aacgaaggca
     1261 aagtcgttgt ggccgcttct actcacatcg ggccagaaac gccgaacaac gatcacgatt
     1321 tgcgctacct caacgcgatt acaaccacgt ggaagattaa cgacaagctt acctcgatca
     1381 ccgatctcaa ttatgcgctg gatgaagtga ctgacgcaaa ggcctacggc atcgctcagt
     1441 atttcaccta cacgatcaac aattggctta cggccggcat tcgcggtgaa atcttccgcg
     1501 atgagcaggg cttttacgtt gtctcgttcg ccgacacgca tgacccgata cgtgttttgg
     1561 caggcgagcc ggccatcgat ccgcgcactg tcggcggcgg gaggaccacc tatggggcgg
     1621 ttacagccgg cgtaaacatc aaaccaccga tgcccaaacc cctggccggc ctgacgatcc
     1681 ggcccgaggt ccgatatgac cgcgcactga cggacacccg gccgttcaac gactcaagcc
     1741 agcgggacca atttaccatt ggagtggatt tcattcttgc cttctaaccc ttgacgatcc
     1801 atgcaggact ttattctctt tctactgata gctgtcgtct tcgcgttgcc actcacttcc
     1861 ctgtggctct gcgagaaggc cgcaaagcgc agaaatgttc gcaaatcaac gcgatcgctc
     1921 ggtcactgga gtcgatctcc tcaccgttac gcctgacaat tttatgaacg accttttttt
     1981 tatcggacta acgattggtt tctttgttct catgctcgtc tttatctggg cgtgcgaaaa
     2041 agtttagccg aagcggagat ccatatattt atgac
//
LOCUS       DASRTJ010000390         5086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_326978,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000390 DASRTJ010000000
VERSION     DASRTJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326978"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFF70_07820"
     CDS             complement(<1..623)
                     /locus_tag="VFF70_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZY44640.1"
                     /translation="MANQARRVSGCVIAGMGIAILAVMATHAPPAKAAGPVLIRGPYL
                     QSVTATSAIVVWRTDGAGDTQVDYGVGSYTYSISDTTIGTEHVASLASLVTGTEVMYR
                     ILTNGTQLYAGSFRTAVDVDTTFSFAVLGDSGVGSTAQYSIANVMTSLDPMFVLHTGD
                     VIYPSGQYEGYDPFFFAPYQTLLRRAPMFLSLGNHDYGTLSGQPYLDT"
     gene            complement(616..951)
                     /locus_tag="VFF70_07825"
     CDS             complement(616..951)
                     /locus_tag="VFF70_07825"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HZY44641.1"
                     /translation="MAIQNRKSAGFWAELIKSFRLAWRLLRDERVPWLIKLIPLIVLI
                     YIVSPIDVIPDFILGLGQLDDLALLILGVQVFIAVSPQAIVQQHRDEIDGVPPSSAGS
                     TAAKEIIDG"
     gene            1069..2196
                     /locus_tag="VFF70_07830"
     CDS             1069..2196
                     /locus_tag="VFF70_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44642.1"
                     /translation="MITTEQIDTSSKAHVRRFIDLPYRLYRHHPQWVPPLRVDMEMML
                     NKKKHPFYEHSAADFFIATRDRRDVGRIAALENRHFNEYHHTHQAQFYLFDCEDDPEV
                     AQALFDQVDAWTRSRQLDCVVGPKGFGALDGYGLLIDGFQHRQMMTMMNYNYAYYQRL
                     VEALGFEKEVDFISCYLNQTSYHLPDRVHSIADRVQQRGTLRVKRFKDKKELRAWADR
                     IGAAYNNAFVNNWEYYPLTPREIKFILDNMMIVADPRLIKIITHGDQVVGFLFGFHDV
                     SAGLQRANGRLLPFGLLNILMDMRRTKWIALNGAGILPEFQGRGGNALLYSEIEKTIK
                     EFGFDHADLTQVADTAVQMRSDLINVGGELYKTHRVYKKSL"
     gene            complement(2234..3037)
                     /locus_tag="VFF70_07835"
     CDS             complement(2234..3037)
                     /locus_tag="VFF70_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44643.1"
                     /translation="MMHRFVNNLRIFWQSAVLSYIALFRWLRPQTYLASKVAMPLWQM
                     LFFTFIGTYGSGASNASFFVIGNAIQITAVSGIFGVTMSVGGDRQEGTLVYLFGTPAN
                     RLTMFVGRALIHIVDGVIGVAIALTWGVVLLGLDLSHTDFPALLLTILITTFSTAGLG
                     LLLGCMSLITLNVMFINNTVYFLLLVFSGANVPVAILPGWMQLISQGLPLTRGIAAAR
                     ILIAGGRLADVLSLLAGELVLGVIYITLGYLMFRWFEVQAKRRGTLEAF"
     gene            complement(3114..3908)
                     /locus_tag="VFF70_07840"
     CDS             complement(3114..3908)
                     /locus_tag="VFF70_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44644.1"
                     /translation="MKLDLLKKYVRVAALSFEITVKSTLVDGFILFGILFQPLIIAIL
                     ALWMLQDRGGEYAIFVVVGSGMTGLWSTLLFACGNSITLERWVGTLEMIIGVPTPLQV
                     IVFGRNLANVLQSLLSMVGSYALISIFFGYHLTIAQPVLFFVSLIFTVISFVCFGLLL
                     APLFVLNPDAQRWQNGLEFPIYILGGFLFPIALLPGWTTPLSYLLAPYWAARALHVAS
                     SGGDVNDVFLSWSVMLIFSVIYVLVSGRMFRSVIRRARVDATLGMQ"
     gene            complement(3898..4875)
                     /locus_tag="VFF70_07845"
     CDS             complement(3898..4875)
                     /locus_tag="VFF70_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44645.1"
                     /translation="MPAIEVKNLRRLFKTNIGVIRRSIKEVVAVDDVSFEVQPGELFG
                     LLGPNGAGKTTSVKMLTTLMIPSGGSARVLGFDVVDQADKIRPRIGFIFGGERGLYWR
                     LSAIDNLRYFAALYQMDPDVTKKRIPYLLELVGLADRGKEKVEGYSRGMKQRLHIART
                     LLHDPEVLFLDEPSIGLDPVAAREMRQTVLDLQAQKKTILLTTHYMFEADALCQRIGV
                     INHGRIIALDTPAGLKKHVQDLSVIEIEVFGVPTATIDRIKAIPGVDAVSIENRDQKQ
                     AMLVQSPRGSAAVPDLMSALREVRVGTVAVREPTLEDAYVRLVGGISEA"
BASE COUNT         1296 a         1439 c         1340 g         1011 t
ORIGIN      
        1 gtatcgagat acggctgacc gctcaaagtc ccatagtcgt gattgcccaa tgagaggaac
       61 atcggcgcgc gccgcagcag cgtttgatat ggcgcaaaga aaaatggatc gtagccttca
      121 tattggccgc tgggatagat cacatcgccg gtgtgcagaa caaacatcgg atcgagcgag
      181 gtcatcacat tggcgatcga atactgtgcg gtcgatccca cgccgctgtc gccgaggacg
      241 gcaaaactga aagtcgtatc cacgtcgacg gcggtgcgaa acgatccggc atacaactgt
      301 gtgccattcg ttagaatgcg atacatcacc tcagtgccgg tcactaaact cgccagggaa
      361 gccacatgct cggtcccgat cgtcgtatcg ctgatcgaat aggtatagct gcccacgcca
      421 taatcgactt gtgtgtcacc cgcaccatcg gtacgccaaa caacgatcgc gctcgtcgct
      481 gtgaccgatt gcaggtaggg tccgcgaatc aagacgggac ccgcagcctt cgcaggcgga
      541 gcgtgcgtgg ccatcacggc caagatcgca atgcccatgc ccgcaatcac acagcctgaa
      601 acgcgccgcg cttgattagc catcaataat ttctttcgcg gccgttgaac cggcactcga
      661 cgggggaaca ccgtcgattt catctcgatg ctgctgcaca atagcctgcg gcgagaccgc
      721 gatgaagacc tgcacaccca agatcaacag cgcgagatcg tcgagctggc cgagtcccag
      781 aatgaagtcg ggaatgacat cgatcgggga aacgatgtag atcaagacga tcaacggaat
      841 caatttaatc agccagggta cgcgttcatc ccgtagcagc cgccaggcta atctaaacga
      901 tttaatcagt tccgcccaga agccggcgga ttttcggttt tgaatggcca tgtcatctcc
      961 gatcgtgatc gagattatac tcgaaaagca ttcgcgacat tggctctttt agggaatcgg
     1021 ctgtataaac aagactgcgg gggacattct ctatgcacag gatcaaaaat gatcaccacc
     1081 gaacagatcg ataccagctc caaagcccat gtccgccgct tcattgatct cccctatcgc
     1141 ttgtatcggc atcacccgca gtgggtgccg ccgctgcgcg tcgatatgga aatgatgctg
     1201 aataaaaaga agcatccgtt ttacgaacat tctgctgcag actttttcat cgccacacgc
     1261 gataggcgcg atgtcggccg cattgctgcg ctggaaaatc ggcacttcaa tgaatatcac
     1321 cacacgcatc aagcgcagtt ctatttattc gactgcgaag acgatcctga agttgcgcag
     1381 gcgttgttcg atcaagtcga tgcctggacg cgatcccgcc agctcgattg tgtggtcggc
     1441 cccaaaggct tcggcgcact cgatggatat ggcctgctga tcgacggctt tcaacatcgc
     1501 caaatgatga cgatgatgaa ctacaactac gcttattatc agcgcttagt ggaagcatta
     1561 ggctttgaaa aagaggttga ttttatctcg tgctacttga atcagaccag ctaccattta
     1621 ccggatcgcg ttcacagcat cgccgatcga gttcagcagc gcggcacatt gcgggtgaag
     1681 cgcttcaaag acaagaaaga gctgcgggcg tgggccgatc ggatcggcgc agcgtacaac
     1741 aatgcgttcg tgaataactg ggaatattat ccattgacac cgcgcgagat caaattcatt
     1801 ctagacaaca tgatgatagt agccgatccg cgcttgatca aaatcatcac ccacggcgat
     1861 caagttgtcg gctttttatt tggattccac gatgtatccg ctggccttca gcgagccaac
     1921 ggtcgattgc tgcctttcgg tttgttgaac atcctgatgg atatgcgccg cacgaagtgg
     1981 atcgcgttga acggcgcggg gattctgccg gagtttcagg gtcgaggcgg caacgcgctg
     2041 ctgtattccg aaatcgagaa gacgatcaaa gagttcggct tcgatcacgc cgatctcacg
     2101 caagtcgccg acaccgccgt tcaaatgcgc agcgatctta ttaacgtcgg cggtgagttg
     2161 tataagactc atcgggtgta caagaaatcg ttatagaaat ttgtagaggc gagcggccgc
     2221 tcgcccttac aaattaaaac gcctctaacg ttccgcgccg cttggcctga acctcgaacc
     2281 agcggaacat taaatagccg agcgtgatat agatcacccc taacactaat tcacccgcca
     2341 gcagcgacaa gacatccgcc aagcgtccgc cagcgatcaa tattctggcg gcagcgatgc
     2401 cgcgcgtcag cggcagcccc tgcgagatga gctgcatcca gccaggaagg atcgcgaccg
     2461 gcacattcgc gccggagaaa accagcagca aaaaataaac ggtgttgttg ataaacatga
     2521 cattgagggt gatcaaactc atgcagccca acagcaaccc gagaccggcg gtgctgaagg
     2581 tggtgatcaa gatcgttaag agcagagcgg gaaaatcagt gtgcgagaga tcaaggccca
     2641 gcagcactac gccccacgtt aaagcgatcg ccacgccgat cacaccgtcg acgatgtgaa
     2701 tcaaggcgcg gccaacaaac atcgtcaatc gattggcggg tgtgccgaac agatagacca
     2761 acgtgccttc ctgtcgatca ccgccaacgc tcatcgtcac gccgaagatg ccactgacgg
     2821 cggtgatctg aatcgcgttg ccgatgacaa agaagctggc gttcgacgcg ccgctgccgt
     2881 aagtgccgat gaaggtgaag aataacatct gccacaacgg catggcaact ttcgaggcaa
     2941 ggtaggtctg cggcctcagc cagcgaaaca gcgcgatgta tgagagcacc gcgctctgcc
     3001 agaagattct aaggttgttg acgaatcggt gcatcatcaa ctctcaatac taaccgataa
     3061 tgcgtactgc gtaatacgta atcagggttt acgcaacacg cagcacgcaa tacttactgc
     3121 atccccaacg tcgcatcgac ccgcgcgcga cggatcaccg atcggaacat gcggccggac
     3181 accaaaacat agatgacgct gaagatcagc atcacgctcc aactcaagaa cacgtcattc
     3241 acgtcgccgc cgctcgaggc gacatgcaag gcgcgggccg cccaatacgg cgccaacaaa
     3301 taactcaacg gggtcgtcca gccgggcagc aaagcgatcg ggaataggaa accgcccagg
     3361 atatagatcg gaaattccaa accgttttgc cagcgctgcg catcgggatt caacacgaac
     3421 agcggcgcga gcagcaagcc gaaacagacg aacgagatca cggtgaagat caaggagacg
     3481 aagaacagca ccggctgtgc gatcgtcaaa tggtaaccga agaagattga gatcaaggcg
     3541 tagctgccca ccatcgacaa gagcgattgc aagacgttgg cgagattgcg gccaaagacg
     3601 atcacttgca gcggcgttgg cacgccgata atcatctcca gcgtgccgac ccagcgttcg
     3661 agcgtgatgc tgttgccaca cgcgaagagc agcgtgctcc acaagccggt catgccgctg
     3721 ccgacgacga cgaagatcgc gtactcgccg ccgcgatctt gcagcatcca caacgcgagt
     3781 attgcaatga tcaacggctg aaataaaatg ccgaacagaa tgaaaccgtc aaccagcgtg
     3841 cttttgacgg ttatttcaaa cgacagcgcg gcgacgcgca catatttttt caggagatca
     3901 agcttcactg atacctccca ccaagcgcac ataggcgtct tccaacgtcg gctcgcgcac
     3961 agcgactgtg ccgacgcgca cctctctaag tgccgacatc agatcgggca cggcggccga
     4021 tccgcgcggc gattgaacga gcatggcttg cttttgatcg cgattctcga tcgagacggc
     4081 atcaacaccc ggaatggctt taatccgatc gatcgtggcc gttggcacac cgaacacttc
     4141 gatctcgatc accgataaat cctgcacgtg cttcttcagg cctgcgggcg tatcgagcgc
     4201 gatgatccgg ccatgattga tcacgccgat gcgctggcac agtgcatcgg cttcaaacat
     4261 atagtgggtc gtcagcaaga tcgttttctt ttgcgcctgt agatcgagga cggtctgccg
     4321 catttcgcgc gccgccacgg gatcgagtcc gatcgacggc tcgtcaagga acagcacttc
     4381 gggatcgtgc agcaacgtgc gagcgatgtg caagcgctgc ttcatgccgc gcgagtagcc
     4441 ttccactttt tctttgccgc gatcggccaa gccgacaagt tcaagcaaat acggaatgcg
     4501 tttcttcgtt acatccggat ccatctgata cagcgccgca aagtaacgca gattatcgat
     4561 cgcgcttaag cgccaatata agccgcgctc gccgccgaag atgaaaccga tgcgcggccg
     4621 aatcttatcg gcttgatcga ccacgtcgaa acccaggacc ctggcgctgc cgcccgaggg
     4681 gatcatcagc gtggtcaaca tcttgaccga agtcgttttg cccgcgccat tcgggccaag
     4741 caaaccgaac aactcgccgg gctgcacttc gaacgagacg tcgtccaccg cgacgacttc
     4801 tttgatcgag cgccggatca cgccgatgtt ggtcttgaac agcctccgta aatttttgac
     4861 ttcgattgca ggcatgatga gggcgccttg agaaaagtaa acccgactta ggagcataag
     4921 cgggtttatg aattacattt attgtacaac aaacttcgat caagagttag aacgaaaact
     4981 taaaatctgt cagtgtcatt catgtcagcc gtggttataa cccttcatcg ccgccgcaac
     5041 accggctccc atttcaaatt tatacccgca gcccgccaag ccgatc
//
LOCUS       DASRTJ010000391         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_327056,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000391 DASRTJ010000000
VERSION     DASRTJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327056"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFF70_07850"
     CDS             complement(<1..470)
                     /locus_tag="VFF70_07850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44646.1"
                     /translation="MDRLNTIEKYTQPEVIEFWRRFSQQGLQKCESEMLARYAPPPAR
                     VIDLGCGAGRVGLALKNNGYAVTGLDLVWPMIRTARDLYAENNIALRLLQADIRSMPC
                     ANDQYEVALIFIAAIQHIPSRAARRSVLAEVARIVRSGGVLILALDNLAPALTC"
     gene            complement(639..1454)
                     /locus_tag="VFF70_07855"
     CDS             complement(639..1454)
                     /locus_tag="VFF70_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZY44647.1"
                     /translation="MNDSFAKVPSGLFIYGPEECYERLEFCPPLKPQQTIDLPEFWIA
                     TSPVTYLEWRSFLEATGYKWSGQWYRVVRGWRGTFLRAYAPTASYPIGHDNFPMVDVT
                     QRDAYAYCAWLSQMIDRRCTLPTEFQWEKAARGIDGRIYPWGNDRPRPEIYWQKKFPV
                     GLETFLFSLIVKPRHEWARAGWYWRNGCPWPIGSKSKNISPYGCLDMSGNIWEWTSSL
                     YNPNLPQFHVVKGGSWGYSIHHAKLNVRSACSVTIPSQDYRAQGTGFRVIIEN"
     gene            complement(1447..>1818)
                     /locus_tag="VFF70_07860"
     CDS             complement(1447..>1818)
                     /locus_tag="VFF70_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44648.1"
                     /translation="LNQRRIDPLALIDPAHAEGHTHHLSTFARTIGDVKIAIGPRPAR
                     KWAAAGPIGLALALALANRGSRNLSALAGAIGSIGYMLGLTGFRRSERSILITAKEAG
                     RSLAIGTALSFLISASQLNNE"
BASE COUNT          423 a          539 c          445 g          411 t
ORIGIN      
        1 taacaggtca gtgccggagc tagattatcg agcgcgagaa ttagcacgcc gcccgatcgg
       61 acgattcgcg cgacttcggc caacacgctg cgccgggcgg cgcggctggg tatatgctga
      121 atcgccgcga tgaagatcaa agccacttca tattgatcat tcgcgcaggg catcgatcga
      181 atgtccgctt gcagcaagcg cagcgcgata ttattttccg catataaatc acgcgctgtg
      241 cgaatcatcg gccatactag atcgagtcct gttactgcat agccgttgtt cttcaacgca
      301 aggccgacac gtcccgcgcc gcagcccaga tcgatcacac gtgctggcgg cggggcgtaa
      361 cgcgccaaca tttccgattc gcatttctgc aagccctgct gactgaatcg tcgccagaac
      421 tcaatcactt ccggctgcgt gtatttctcg atcgtgttca ggcgatccat gattcaattt
      481 ccattgtcat ttcgagcgca gcgagaaatc tctatctcga taaactcttg acgctgatcg
      541 gcgctgagat ttcggccata cccaacgccg aatggcgttg gtggcccgca aggcgtcggc
      601 ttccggcctc gaaatgacaa ccgagaataa atacttcatc aattctcaat gatcactcta
      661 aagccggtgc cttgcgcgcg atagtcttgg ctggggatcg ttacgctaca cgccgatcga
      721 acattcagct tcgcgtgatg aatgctgtaa ccccacgatc cgcctttgac cacatgaaac
      781 tgcggtagat taggattata caagctgctc gtccattccc aaatattacc actcatatcg
      841 aggcagccgt acggcgaaat atttttggac ttcgagccga tcggccacgg acaaccgttc
      901 cgccaatacc aacccgcgcg cgcccattca tggcgcggct taacgatcaa actaaataaa
      961 aaagtttcaa gcccaaccgg aaattttttc tgccagtaaa tttccggcct cggccgatcg
     1021 ttgccccacg gataaatccg tccatcgatc ccgcgcgccg ccttctccca ttgaaactct
     1081 gttggcaaag tgcagcgccg atcgatcatc tggctcaacc aagcgcagta cgcataggca
     1141 tcgcgctgcg ttacatcgac cattggaaaa ttatcgtgtc caatcggata actcgccgtc
     1201 ggcgcatacg ctcgcaaaaa agttccgcgc cagccgcgca ccactcgata ccactgcccg
     1261 ctccacttgt agccagtcgc ttctaaaaaa cttcgccact ccaaataagt gactggcgag
     1321 gttgcgatcc aaaattccgg cagatcgatc gtttgctgcg gtttgagcgg cgggcaaaat
     1381 tctaatcgct cataacattc ttcaggaccg taaataaata agcctgatgg aactttggcg
     1441 aatgaatcat tcattgttga gctgcgacgc tgatatcaag aaacttagcg ctgtgccgat
     1501 cgctaaactt ctgcccgcct ctttcgctgt gatcaagatc gatcgttccg atcggcgaaa
     1561 gccggtcaga cccaacatat agccgatcga tccgatcgcg cccgccagtg ccgataaatt
     1621 ccggctgcct cgattggcta gtgcgagggc caacgctaag ccgatcggac ctgcggctgc
     1681 ccattttcgc gcagggcgtg gtccgatcgc tatcttcacg tcgccgatcg tgcgcgcaaa
     1741 tgtcgagaga tgatgcgtgt gcccttcggc atgcgccgga tcaattagcg ccaacggatc
     1801 gatccggcgt tgattcaa
//
LOCUS       DASRTJ010000392         7549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_62280,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000392 DASRTJ010000000
VERSION     DASRTJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62280"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /gene="glyA"
                     /locus_tag="VFF70_07865"
     CDS             complement(<1..1589)
                     /gene="glyA"
                     /locus_tag="VFF70_07865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZY44649.1"
                     /translation="MSVSNDFLFRGDLAAIDPAVAELINHETARQIRKLIMIPSESTA
                     PQAVREVLMSPLQNIYAEGYPDPRTRAQNETQILDYDEQLAYYRRYGDPRYYKGVEYA
                     DMVEALARRRTAEAFATDIIGPGRIFVNVQPLSGAPANNAVYEALVKPGDTVMGMSLI
                     AGGHLSHGSPVNRSGKHYNIVAYDVDHETELLDYDKIREQALQAKPKMIIAGYTSYPW
                     APDWKKFREIADEVGAYLMADISHTSGLVIAGAYPSPLGLAHVITFTTHKTLVGPRGA
                     CIVTLEEALSRKIDRAVFPGEQGGPHMNNIAAIAVAMKLARTAEFKNLQLQTVKNSER
                     LAKALTDNGIRVAYGGTNSHLLLIDCKPVKGADGTPLMGDPAARILDLAGIVANRNTL
                     PGDVSAGAASGVRMGTSWITQRGAKEAEIDRLGELIANLLKACTPYTYDTKGGGEAYR
                     SKVDFNVFEQTKIGVADLIDKLGIDVTAAMAGYPHYWSLNDKYEAKTMVFDVTGDKAY
                     SLLQDATTNDVDLLKDGQTQPT"
     gene            complement(1784..1857)
                     /locus_tag="VFF70_07870"
     tRNA            complement(1784..1857)
                     /locus_tag="VFF70_07870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1821..1823),aa:Val,seq:tac)
     gene            complement(2084..2644)
                     /gene="dcd"
                     /locus_tag="VFF70_07875"
     CDS             complement(2084..2644)
                     /gene="dcd"
                     /locus_tag="VFF70_07875"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZY44650.1"
                     /translation="MTIQPDRWIRKMATEHGMIEPFVEKQVSNHVISYGVSSYGYDVR
                     VADEYKIFTNVYSAVVDPKQFDARSMVDFQGDVCVIPPNSFVLARTIEYFRIPRNVLT
                     ICLGKSTYARCGLIVNVTPFEPEWEGFVTLEISNTTPLPAKVYSNEGIAQVLFFVADE
                     ECETSYKDRKGKYDKQIGVVLPRVIK"
     gene            complement(2866..3108)
                     /locus_tag="VFF70_07880"
     CDS             complement(2866..3108)
                     /locus_tag="VFF70_07880"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HZY44651.1"
                     /translation="MAEICADSLTKDALWQFNLYPKTVFLYDLVWEDCEKLLKDARGR
                     AIAAQIIRSTGSISANIEEGFGRGFGKEFGYFLNVA"
     gene            complement(3194..4891)
                     /gene="argS"
                     /locus_tag="VFF70_07885"
     CDS             complement(3194..4891)
                     /gene="argS"
                     /locus_tag="VFF70_07885"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZY44652.1"
                     /translation="MSVLIRDQIIDLLRDALINAQRDKSLPEFELPSIELARPKQAAH
                     GDYSSNLGLALAKTAKLPPQQIAAIVKEHIVAANFIQQIDIAPPGYLNIALTDRWLTE
                     QVDAIVKTGSAWADLSIGNGQRVQVEHGSANPTGPITIGSARNVTIGDTIANLLQACG
                     YDVQREYYVNDAGSQIRHFGESIYARYAQALGKDEEFPEDGYKGAYVADIARALANEH
                     GHKFLGIVKQAAIRELGKLALDQMVEDAKRTLARANIQYDRWFSERSLYESGLFDRTL
                     ALLRTKDLIYEQDEATWFKATQFGADKDAVVIRSAKVIPEPIERPTYFGSDIAYVYDK
                     FMDRNFDRVIYVWGADHHGDVPRMAAIRQVYGIDASRLKIVLYQLVNITRGGEEVRQS
                     KRSGDFLTLDEVIDEIGSDAIRFMLLTRTVDSKLTLDLDLAKEQSDKNPVYYVQYAHA
                     RIASIVRKADRDLAEMKLPRADVQLLTHPAELTLIRKMLELPVVIELAARDLAPHHLT
                     YYAQELASTFSAFYRDCKVVDAEAPAVTAARLQLCTAAKITLAKTLSLMGMSAPESM"
     gene            5165..>7549
                     /locus_tag="VFF70_07890"
     CDS             5165..>7549
                     /locus_tag="VFF70_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZY44653.1"
                     /translation="MDQLLVYMPTDRRYAAARGESLPDRMAGAALFADISGFTPLTEA
                     LARALGSRHGAEELTRQLNKVYDKLIAEVDNYRGSVIGFAGDAITCWFDDSDGPASLR
                     AVQCAFRMQQAMQHFASIVLPDGTTTALAMKASVATGDARRFMVGAPELQLIDAIAGS
                     TVARMALGEHHAEKGDVIVDAATIDRLGEAVSIKEWRVDEDSGDRLAALDRLITPIES
                     EPWPDLPSNAIDIDQMKYWLLPGVFDRMNAGLGEFLMELRPAVSVFLRFEGIDYDHDA
                     AAEKKLDAYIRWVQKSFTRYEGNLLQLTIGDKGSYLYGAFGAPIAHEDDARRAVSVAL
                     ELRSIPPELNYIQPVQIGISRGTMRTGGSGSSTRRTYGVLGDEVNLAARLMQHAAPGE
                     VLVSGYVHKIVPNGFVWQTLEPITVKGKSEPVPIFRALGKQLTTTETSSFTRTLVGRK
                     QELAQLASFARPILAERFAGLMVVYGEPGVGKSRLVYEHRQQLNQLGRHVAWYTALSE
                     GILRSALHPFRYFLQRYFDQTPERSGDENKNKFTDTLDRLIDSLSAVEDVNRSAVEDI
                     ASELDRTRSFLGAQVDLYWPGSLYDQLPPRQRFENTLSAFKTLIEAESMLHPVVLHIE
                     DAHYLDADSKQLIEILTRSAAAYPFALLLTSRYRDDGSRISFEVDADVPEQMIDLNPL
                     SSEDVKTLSAQVLGGEISQALTAFLAEKTQGNPFFLEQFTSELRDRNLLQQDNGQWAM
                     LSTGMQEVPTSINALLIARLDRLTARVKAVVQTASVLGQEFEVRVLSEMLKDDQY"
BASE COUNT         1654 a         2040 c         2099 g         1756 t
ORIGIN      
        1 gtcggctgcg tctggccgtc cttcaacaga tcgacatcgt tcgtcgtcgc gtcttgcaac
       61 agactgtagg ctttatcgcc cgtcacatcg aacaccatcg tcttcgcttc atacttatca
      121 ttcagcgacc agtaatgcgg atagccggcc atggcggccg tcacatcgat gcccaacttg
      181 tcgatcaagt ctgccacgcc gatcttcgtt tgctcgaata cattgaaatc gactttcgat
      241 cgataggctt cgccgccgcc ctttgtgtcg taggtgtatg gcgtgcaggc cttcaacaaa
      301 ttcgcgatca attcgcccag ccgatcgatc tcggcttcct ttgcgccgcg ctgcgtgatc
      361 cacgacgtgc ccatgcgcac gccagaggcc gcgcccgcgc tgacatcgcc cggcagcgta
      421 tttcgattcg cgacgatgcc tgcaagatcg agaatgcgcg cggcgggatc gcccatcaag
      481 ggtgtgccgt ctgcgccttt gaccggcttg caatcgatca acagcagatg cgagttcgtg
      541 ccgccgtagg ccacgcgaat gccgttatcg gttaaagcct tcgccagacg ttcagagttt
      601 ttaaccgttt gaagttgcaa gttcttgaat tcagcagtgc gagccagttt catcgcgacg
      661 gcgattgctg cgatgttatt catgtgcggc ccgccttgct ccccggggaa taccgcccga
      721 tcgatcttcc ggctcaatgc ttcttccaaa gtgacaatac acgcgccgcg agggccgacc
      781 aatgttttgt gtgtggtgaa ggtgatgacg tgtgccaagc ctaatggact tggataagcg
      841 cccgcgatta ctaaaccgga cgtatgcgaa atgtcggcca ttaaatatgc gccgacttca
      901 tccgcgatct cgcggaactt tttccaatcg ggtgcccacg gatacgaggt gtagcctgcg
      961 atgatcatct tgggcttggc ttgcagtgct tgctcgcgaa ttttgtcgta gtccaacaat
     1021 tcggtttcat gatcaacatc ataagcgacg atgttgtagt gcttgcccga tcgattgacg
     1081 ggcgagccgt gtgataagtg tccgccggcg atcaacgaca tgcccatcac cgtgtcaccg
     1141 ggttttacta acgcttcata gactgcattg ttagcaggtg cgccagagag tggctgtaca
     1201 ttcacgaaga tgcgaccggg accgataata tcggtggcga atgcttcagc ggtgcggcgg
     1261 cgagcgagcg cttcgaccat gtcggcatac tccacgcctt tgtagtaacg cggatcgccg
     1321 tagcgccgat agtaagcgag ctgctcatcg taatccagaa tttgcgtctc gttctgcgcg
     1381 cgcgtgcgcg gatcgggata cccttcggcg taaatgtttt gcagcggcga catcaatact
     1441 tcgcgcaccg cttgaggtgc agtggactcg ctcgggatca tgatcaactt gcgaatctgg
     1501 cgcgccgtct cgtgattgat caactcggcc accgccggat cgatcgcggc taaatccccg
     1561 cggaacagaa aatcgtttga aactgacatg gtgactccta ttgaggatgt gaactcgcct
     1621 tagaaggcga gttgaggtgc gaagaattaa gacgatgata ttgtaacacc agcccaaggc
     1681 agggtcaacg gagtttcatc atatggaaaa tggacaatcc tcattgcggt tttggcaaga
     1741 aaaacaaaaa accgccagca tcgatcggcg gttattgtta ttgtgggcga aataggactc
     1801 gaacctacga ccccctcggt gtaagcgagg tgctctaacc agctgagcta ttcgcccgtg
     1861 gcaatgatta taagcgggcg agggggatcg gtcaaggaaa cctaattttg ttgaaactca
     1921 agaccgatcg attgggcggc ctcatcaagc gatcaggatc ggtgcgatta cgcacagaaa
     1981 ttagtggaag gcgaaagcgg agaatgataa tcgaacctga cgcgaggagg gaggcgcagc
     2041 gatcaatttc gtcgctgcgc ttcgcgcctt tcaactgctc tatttacttg attactctcg
     2101 ggagcacaac ccctatctgc ttgtcatact tgcctttgcg atctttgtac gacgtctcgc
     2161 attcctcatc ggcgacgaag aacagcacct gcgcaatgcc ttcgttcgaa tacactttcg
     2221 cgggcaaggg cgtggtgttg ctgatttcga gcgtgacgaa accttcccac tccggctcga
     2281 agggcgtcac gttgacgatc aagccgcagc gggcgtaggt cgatttgccc agacagatcg
     2341 tcagcacatt gcgcgggatg cgaaaatatt cgatcgtccg tgccagcacg aacgagttcg
     2401 gcggaatgac gcacacgtcg ccctgaaagt ccaccatcga tcgtgcgtcg aactgtttcg
     2461 gatcgaccac ggcggaatac acattggtga aaatcttgta ttcgtcggcc acacgaacgt
     2521 catagccgta cgacgatacg ccatacgaga taacgtgatt gctgacttgc ttctcgacaa
     2581 acggctcgat catgccatgc tcggttgcca tcttacgaat ccagcgatcg ggttgaatag
     2641 tcatactgct ccttgacgtt agatgttaga tatcactatg caagttgcaa gtggcaagtt
     2701 gaagacggtt tacattttac gtttaggtcg gtgtctttga taggcaacat tcttaacgag
     2761 tccaccaata atttcatcca gtaaatgtaa acgatgttcc aagacttcag gtggcaataa
     2821 tcgccgggca cgataatacc agccgcgagt ttcgcgggct gagcctcatg ccacattcaa
     2881 gaaatacccg aactcctttc caaatccgcg cccgaatcct tcttcaatgt tggctgaaat
     2941 cgatcccgtg ctgcgaataa tttgcgctgc aatcgcgcga cctcgcgcat ctttcagtaa
     3001 cttctcgcaa tcttcccaca ccaaatcata caagaataca gtctttggat aaagattgaa
     3061 ctgccacaaa gcatcttttg ttaggctgtc ggcacagatt tctgccattc atcaaagttc
     3121 ataccagcct cccttgcatc gctcaatttg gcatcattca attgctatcc gctacttgca
     3181 acctgcaaca tgcctacata ctctctggtg cactcattcc cataaggctg agtgtttttg
     3241 ctaaggtgat cttcgccgca gtacacaact gcaagcgcgc ggccgtgacg gctggcgctt
     3301 ccgcatcgac gaccttgcaa tcgcgataga aggcgctgaa ggtcgaggcg agttcctgcg
     3361 cgtagtacgt caggtgatgc ggcgcaagat cgcgcgcggc caattcgatc acgacgggta
     3421 gttcgagcat cttgcggatt aacgtgagtt cggccggatg ggtgagcagt tgcacatcgg
     3481 ctcgcggcaa tttcatctcc gctaaatccc ggtcggcctt gcggacgatg ctggcgatgc
     3541 gggcatgcgc atattgcaca tagtagaccg ggttcttgtc cgattgttcc ttcgccagat
     3601 cgagatcgag cgtcagcttg ctgtcgaccg tgcgggtcag cagcatgaac cggatcgcgt
     3661 ccgaaccgat ctcgtcgatc acttcgtcga gcgtcaggaa gtcaccgctg cgtttggact
     3721 gccgtacttc ttcaccgccg cgcgtgatgt tgaccaattg atacaatacg atcttcaagc
     3781 ggctcgcgtc gatgccgtag acctggcgga tggctgccat gcgcggcaca tcgccgtgat
     3841 gatccgcgcc ccagacatag atcacgcgat cgaaattacg atccatgaac ttgtcgtaca
     3901 cgtaagcgat gtcgctgccg aagtacgtcg gccgttcgat tggctccggg atcactttgg
     3961 ccgaacggat gacgaccgca tctttgtccg cgccgaactg ggtcgctttg aaccaggtgg
     4021 cctcatcttg ctcgtagatc aaatctttgg tgcgcaataa agccaacgtt cgatcgaaca
     4081 agccggactc atacaacgat cgttcgctaa accagcgatc gtattggatg ttggcgcgtg
     4141 ctaaagttcg tttggcatcc tcgaccatct gatcgagtgc cagcttgccc aattcgcgaa
     4201 tcgcggcctg cttaacgatg ccgagaaact tatggccgtg ttcattagcc aacgctcgag
     4261 caatatcggc aacgtacgcg cctttgtaac catcttccgg aaattcttca tctttgccta
     4321 aagcttgcgc atagcgcgca tagatcgatt cgccgaaatg ccggatttgc gagcccgcat
     4381 cattcacgta gtattcgcgc tgcacgtcgt agccgcaggc ttgcaacaga ttcgcgatcg
     4441 tgtcgccgat cgtcacattg cgcgcggagc cgatcgtgat cgggccggtg ggattcgccg
     4501 agccatgttc cacttgcaca cgttgcccgt tgccgatcga gagatcagcc cacgccgatc
     4561 cggtcttaac gatcgcatcc acttgctcgg tcagccagcg atcggttaac gcgatgttga
     4621 gataacccgg cggggcgata tcgatctgct ggatgaagtt ggcagcgacg atatgctctt
     4681 tgacgatggc ggcgatttgc tgtggtggca gtttggcggt tttggccagt gctaaaccta
     4741 agttgctgga ataatcgcca tgcgcggctt gtttgggtcg cgctagctcg atcgagggca
     4801 gctcgaactc cggtagagat ttatctcgct gcgcgttgat gagagcgtca cgcaagagat
     4861 cgatgatttg atcgcgaatg agtacactca taagttgctc ctcaatatag acaagcccgc
     4921 ctcagcggcg ggcttgtcta tattgtatcg actttgcaat gaatcgacaa ggtcaagagc
     4981 ggccagatca tcaagaaggt ggattggatc aaagcgagaa gcaactgaaa acaaaaacgc
     5041 cgatcagccc gcactgatct cttcgattgt ttgcggattg gctggaatcc agatacaata
     5101 ttggcgctgt cctcatagcc aaaatttgcg ggttgcggtt caaactgctt cgggatcgat
     5161 ctgaatggat caactgctgg tctacatgcc caccgatcgg cgctatgcgg cggcacgcgg
     5221 cgaaagcttg cccgatcgaa tggcaggcgc agcattgttt gccgacatct ccggttttac
     5281 tccactcact gaagcgttgg cgcgcgcctt gggttcgcgt catggcgcgg aagaactcac
     5341 gcgacaactc aataaagtgt acgacaaatt gatcgccgag gtcgataact atcgcggcag
     5401 cgttatcggc ttcgccggtg atgcgatcac gtgttggttc gacgacagcg acggtccggc
     5461 ctcactgcgc gcggtgcaat gcgcgtttcg catgcagcaa gcgatgcagc atttcgcgtc
     5521 gatcgtctta cccgatggca cgacgacagc attggcaatg aaagcctcgg tcgccaccgg
     5581 tgatgcgcgc cggttcatgg tcggcgcgcc cgaacttcaa ctgatcgatg cgatcgccgg
     5641 atcgactgtg gcgcgtatgg ccttaggcga acatcatgcc gagaagggcg atgtcatcgt
     5701 cgatgcggcg acgatcgatc gattaggcga ggcggtgtcg atcaaagagt ggcgggtgga
     5761 tgaagacagc ggcgatcggt tagcggcact cgatcggctg atcacaccga tcgagtcgga
     5821 gccgtggcct gatctcccct cgaacgcgat cgatatcgat caaatgaagt attggttatt
     5881 acccggcgta ttcgatcgta tgaatgcagg gctgggcgag ttcttgatgg aattgcggcc
     5941 ggctgtttcg gtcttcctgc gtttcgaagg gatcgactat gatcacgatg cggccgccga
     6001 gaaaaaatta gatgcctaca ttcgctgggt gcaaaaatct ttcacgcgct atgaaggcaa
     6061 cttattgcaa ttgaccattg gcgataaggg cagttacttg tacggcgcgt tcggtgcgcc
     6121 gatcgcgcat gaagacgatg ctcgccgcgc ggtcagcgtc gcgctggaac tccgcagtat
     6181 tccacccgaa ttgaattaca ttcaaccggt tcagatcggc atcagtcgcg gcaccatgcg
     6241 caccggcggc tccggcagtt cgacgcgccg cacgtatggc gtgttgggcg atgaagtgaa
     6301 tctggccgcg cgcttgatgc agcatgccgc gcccggtgaa gtgttggtca gcggttacgt
     6361 gcataagatt gtgccgaatg ggtttgtctg gcagactttg gagccgatca ccgtcaaagg
     6421 caaatcagaa ccggtgccga tctttcgtgc actcggcaaa cagctgacca ccaccgagac
     6481 ttcatcgttc acgcgcactt tggtcggacg caaacaagag ttggcgcagc tcgccagttt
     6541 tgcgcgaccg attttagccg agcgcttcgc gggtctgatg gttgtgtatg gcgaaccggg
     6601 cgtcggcaag agccgcctcg tgtacgaaca tcgccagcaa ttgaatcaac taggccggca
     6661 cgtcgcctgg tataccgcgc tgagcgaagg catcctgcgc tcagcgctgc atcccttccg
     6721 atatttcctg cagcgctact tcgatcaaac gccagagcga tcgggcgacg agaataaaaa
     6781 taaatttaca gatactcttg atcgtttgat cgacagcctg tcagccgtcg aggacgtgaa
     6841 tcgatcggcg gtggaggata tcgcctccga gctggatcgc acccgatcgt ttttgggcgc
     6901 gcaagtggat ttgtattggc ctggctcgct gtacgatcaa ttgccgccgc gccagcggtt
     6961 tgagaatacc ctcagcgctt tcaagacgtt gatcgaagcc gaaagtatgt tgcatccggt
     7021 ggtgctgcac attgaagacg cgcattatct ggacgccgac tctaagcaat tgatcgaaat
     7081 cttgacgcgc agtgcggctg cttatccgtt cgccttgctg ctgaccagcc gctatcgcga
     7141 tgatggcagc cgcatcagtt ttgaagtcga tgccgatgta ccggagcaaa tgatcgatct
     7201 gaatccgctg tccagcgaag atgtgaaaac gttgtcggcg caagtgctgg gtggcgagat
     7261 cagccaggcg ttgacggcgt tcctcgccga gaagacgcag ggcaatcctt tctttctgga
     7321 acaattcact tccgaactgc gcgatcgaaa tttgctgcaa caagacaacg gccagtgggc
     7381 aatgttgagt acgggcatgc aggaagtgcc gaccagcatc aacgcgctgc tcatcgcgcg
     7441 tctggatcga ttgacggcgc gtgtcaaagc cgtggtgcaa accgcttcag tgttgggcca
     7501 ggaatttgaa gtgcgcgtgc tgtccgagat gctcaaagac gatcagtat
//
LOCUS       DASRTJ010000393         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_351021,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000393 DASRTJ010000000
VERSION     DASRTJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351021"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFF70_07895"
     CDS             complement(<1..580)
                     /locus_tag="VFF70_07895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44654.1"
                     /translation="MRNLSRVSPLAIMVALLFAAAALRIIGWLDAPPGLRYDEMTVVV
                     EADDIRLGDRPIYMDGSAEEPLYHYLFAIVEDTIGPSLFAQRWLSAMLGLIAVAASYA
                     LGRSLFHVRVGLLAAAFSVAAFWALMYSRMGLRIVALPAFVLISMLFLWRGFNLAKVS
                     VANENLQAQPSQAYSRYFIIAGIIFGLSAYTYS"
     gene            complement(675..1130)
                     /locus_tag="VFF70_07900"
     CDS             complement(675..1130)
                     /locus_tag="VFF70_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HZY44655.1"
                     /translation="MKKLNLAREVMWSYFGEEIDRSGNGIKLEARFNRETSDAGYVVF
                     ERNDRLVEYFYADRWYNIFEIHSVWDDHLKGWYCNIAEPAVFLEEAIEQVDLALDVWI
                     SPDGTTLILDEDEFTELMLDDETRQQAHRAVQELIALVQQRNTPFDSIK"
     gene            complement(1148..2251)
                     /locus_tag="VFF70_07905"
     CDS             complement(1148..2251)
                     /locus_tag="VFF70_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44656.1"
                     /translation="MSLLETKGINKSFTGLPVLHDVSVSVESGEIVCLLGPSGCGKTT
                     LLRIIAGLEQADSGTVVFEGRDLKDVPIHQRGLGLMFQDFALFPHKNVWDNVIFGLRM
                     QNLDHDQILRQGTSALDLVGLSGFEARDVNELSGGEKQRVALARSLAPQPKLLMLDEP
                     LGSLDRKLREELMIELRRILKAAQLTSIYVTHEQQEAFAMADRVVIMNAGRIEQVGSP
                     QSVYNRPGTEFVARFLGLPNLINGTVASTTTLVASVLTPVGLLNIRIDAPRSLLRNQA
                     ITVLIRPDAASIDQNPFAQDADTSAEWFAETPQANTIDGVLIDSSFRGRYNRVVVKVN
                     EVDLTFEIEVRSDLPRIGEHITLSINSDAIVIL"
BASE COUNT          588 a          619 c          581 g          520 t
ORIGIN      
        1 ccgagtaggt gtaagcgctc agtccaaata tgattccggc gatgatgaag taacgtgagt
       61 aagcttgcga aggctgggct tggagatttt cattcgccac tgaaaccttc gcaaggttaa
      121 agccgcgcca caaaaagagc atagatatga gcacgaaggc aggcagggcg acaatgcgca
      181 gacccatacg gctatacatc aacgcccaaa acgcggcgac cgaaaacgct gccgccaaca
      241 atccaactcg cacgtgaaac aagcttcggc ctaaagcata gctcgcggcg acggcaatca
      301 agcccagcat cgccgacaac cagcgctgcg caaagagact ggggccgatc gtgtcttcga
      361 cgatcgcaaa tagataatga tacagcggct cttccgccga gccatccatg tagatcggtc
      421 gatcgcccaa gcgaatatca tcggcctcga cgacgaccgt catttcgtca tagcgcagac
      481 cgggcggcgc atccagccag ccaatgatac gcaacgcagc cgcagcaaac aacagggcga
      541 ccatgatggc aaggggtgaa acacgcgaga ggtttcgcat cgcggcaact atactcgtga
      601 accgaaaagc atgcaatcaa ttggacacgg atttacgcgg atattacgga taagaaaata
      661 aatctgcgtc tgatctattt gattgaatca aacggggtat tgcgttgttg aacgagggcg
      721 atcaattctt gcacggctcg atgagcttgt tgacgcgttt cgtcatcgag catgagttcg
      781 gtgaactcgt cttcgtcgag gatcagcgtg gtgccatccg ggctgatcca cacatcgagc
      841 gcaagatcga cttgctcgat ggcctcttct aaaaatacgg ccggttcagc gatgttgcag
      901 taccagccct tgaggtgatc gtcccacacg gaatggatct cgaagatgtt ataccaccga
      961 tcagcgtaga aatattccac caaacgatcg ttcctctcaa ataccacgta gcctgcatcg
     1021 ctagtctcgc gattgaaacg cgcttcgagt ttgatcccat ttcccgatcg atcgatctcc
     1081 tcgccgaaat acgaccacat cacttcgcga gcgagattca gtttcttcat ctcaatcgat
     1141 ttacccatta cagaatgaca attgcatcgc tattaatcga caacgtgatg tgttcaccga
     1201 ttctcggtaa atctgatcgg acttctattt cgaatgtgag atcaacttca ttcactttga
     1261 ccaccacgcg attgtatcgg ccgcgaaacg aagaatcgat caaaacgcca tcgatcgtgt
     1321 tggcctgcgg cgtttcggca aaccattcag cggaagtatc ggcatcttga gcaaatggat
     1381 tttgatctat tgacgcggca tcaggtctga tcagcacggt gatggcttga ttgcgtaaga
     1441 gtgatcttgg cgcatcgatc cgaatattga gcaagccgac tggcgtcaac accgatgcaa
     1501 ccagagttgt cgttgaggcg actgtgccat tgattaagtt cggcaatccc aaaaagcgtg
     1561 cgacgaactc tgtacccggc cggttgtata ccgattgagg cgatccaacc tgctcgatgc
     1621 ggcctgcgtt catgatcacc actcgatcgg ccatggcgaa ggcctcctgc tgttcgtgtg
     1681 tcacgtaaat cgaggtgagt tgtgccgctt tcaaaatgcg ccgcagttcg atcatcaact
     1741 cttcgcgcag cttacgatcg agcgaaccca gcggctcatc gagcatcagt aacttgggtt
     1801 gaggtgcaag gctgcgagct aacgccacgc gctgcttctc gccgccggac aattcattca
     1861 catctcgcgc ttcaaatccg ctgaggccga cgaggtcgag tgcagatgta ccttgccgca
     1921 ggatttgatc gtgatcaaga ttctgcattc gcagtccgaa gatcacgtta tcccacacgt
     1981 ttttgtgtgg aaacaacgca aagtcctgaa acatcaagcc gaggccgcgt tgatgaatgg
     2041 gcacgtcttt tagatcgcgt ccttcaaaaa caactgtgcc cgaatccgct tgttccaagc
     2101 cggcgatgat acgcagcagg gttgtcttgc cgcagcccga cgggccaagc aagcatacga
     2161 tttcgccgct ttcgacgctc acggaaacat cgtgtaaaac tggaaggcca gtgaatgatt
     2221 tgttgatacc tttggtttcg agtaatgaca tgcgctgatt atatcctcaa aactcgccga
     2281 cttcgccaaa tctaaatcgt tcgatcgt
//
LOCUS       DASRTJ010000394         6658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_123826,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000394 DASRTJ010000000
VERSION     DASRTJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123826"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..690)
                     /locus_tag="VFF70_07910"
     CDS             complement(145..690)
                     /locus_tag="VFF70_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZY44657.1"
                     /translation="MSYRSISFISLAVTLAACGSNAPQIDPGSTAVTFTLSSPAFSMG
                     DLVPKDITCTGSNRSPQLSWSSAPSATQSFVLIMDDPDAPGGTFTHWLLFDIPVASSI
                     LLEAESTIGLSGTNSFGQSNYQGPCPPIGRGAHRYFFTLYALNISSLHLKNGASRNQV
                     EAAIESHIVGQAQLMGKYERK"
     gene            complement(737..1972)
                     /locus_tag="VFF70_07915"
     CDS             complement(737..1972)
                     /locus_tag="VFF70_07915"
                     /inference="COORDINATES: protein motif:HMM:NF023288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HZY44658.1"
                     /translation="MADPIFVQPKAGSGGVTLRSQALIDPATKVGGLKEGVRLELDLQ
                     GTAWHTCRVYVVAQFAALSADGKSFTPLGGSAVNLRSINSANDPGNVVGILNSGQSLE
                     LISLLDTWFVGRVYASAQFTDVLAPGQQPTASFGAIFNPADFINAVLTPNSQRTTPAN
                     AGFKAVVASKIWNMYGGLIETLSQKINIDSGIAVAVIATESGGQASGAAGRMTIRFEN
                     HLFWFRWGKENADVYNQLFQFDATVNWQGHQYRPNTNSPWLNVHANQDSEYAALGVAR
                     SLNDSLALSSISMGLVQILGSNFAACGYSSIPAMFDSFSTDPRNQLIGFFNFVVNDSV
                     KVTAMQQKDYATFATRYNGSGQVELYAGLIRDYVDAFNSLIVQPSMDSTPPPTTETAP
                     ATTPSQPVKGMANTLKKSS"
     gene            complement(2213..4354)
                     /locus_tag="VFF70_07920"
     CDS             complement(2213..4354)
                     /locus_tag="VFF70_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44659.1"
                     /translation="MVCAANTNCGDVLLQILIYGISNGAVLALNAISITVIYGTVRTL
                     NLAHGDVFALTTVVITTLVRALGLQPDSSALVLIGGLAITLGVGILFGTGLSVAIERI
                     AFKPFRGRSRLAPLIATLGISFMLFQVAMIWRTLMPSWVWHEHRSVPGLNEVPTDGIP
                     TLLPNINLVHALGIDSTVNFNFANLFILVVAVAGAIGASLYLKRSKTGRAIRAASQNP
                     QLAQINGVNLNSAIRRAFAFGGALAGVAAFIFAMYYTRPFGAAGAESGLMAFAAAILG
                     GVGSPIGALVASMGLGIFSAFSDYFLSAQWTPVLLLILFIAVLCWRPTGLAAEESNEE
                     AASQTIRDSVILTAPGKQRSNRWLIAIFLGLALFPIVSSITGLGWQSLVRIVSIYALI
                     ALGLNMLLGVAGILDLGFAVSFGIGGYTVALLANPYGLIGRLIPQPIDFTILILISAI
                     FAGLYGAVKGGLARRLHSDYFAVATLALGLLAERVINNLRDLTGGAGGIGALPPPRIL
                     SVPIEGQTQQYYFVFVVVLIVALFAQRLIRSRTGRAWIAASDDETAAASTGVNVDRYK
                     TLALIVSSVIAGVAGALYASTFAFVSPDMVAFHVSALALSMVILGGAGSIPGAVLGAM
                     LIIGYDQIIIPQFAAFVAQFWPSNSFIGSVPDLRGTSFFNFGLALYLTVWLRARRRGN
                     ENDSQTKPPSRRWFHSRLLKPAPSIRQDTPA"
     gene            complement(4394..4738)
                     /locus_tag="VFF70_07925"
     CDS             complement(4394..4738)
                     /locus_tag="VFF70_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY44660.1"
                     /translation="MANSSITLLRPKVIQLDPGDQSYFKLLGGPPETRSIKSGLVTLS
                     PGKSIGLHSTQSNEEVIVPLAGCGQLRLADHKPIDLSTGLIAYVPPQTEHDVINTGTV
                     DLRYIFIVAKAQ"
     gene            complement(4881..5951)
                     /locus_tag="VFF70_07930"
     CDS             complement(4881..5951)
                     /locus_tag="VFF70_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HZY44661.1"
                     /translation="MDFGITFKSDIPIPRAIKLAQAAEAGGFSYIWAFDSHVLWQEVY
                     MQLTVLGMNTKKMKLGPCVTNPRVRDITVTASTFATLQRFTGGRMQLGIGRGDSSRRV
                     MGKKPTTVEILEQSIQEFRDLTAGKQITFEKQPIQLTWADGGVPPVWVAGYGPKALGM
                     AGRVADRIILQFADPHLIKWCMEMVKAGADQSGRDFSKIKIMSAAPIWISDDMKVARD
                     HVRWFPALVSNHVVDLISKCDPAQLPQELTAYVRDRKGYNYLHHAEVGSSNAEFVTDE
                     IVDRYDIVGPISEHIRKLEELRDLGVHQFNIYLMCGDEEEQVQAYSKYVIPHFNKMSV
                     RVVKPKAIPTAKKKVAAKRRRR"
     gene            complement(5992..>6658)
                     /locus_tag="VFF70_07935"
     CDS             complement(5992..>6658)
                     /locus_tag="VFF70_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZY44662.1"
                     /translation="PDGPTTKLAQDLAKKLKMVLIVPMYEEEQTGMYYNTAAVIDADG
                     KFLGKYRKTHIPQVNGFWEKFYFRPGNLGYPVFDTAVGKVGVYICYDRHFPEGPRMLG
                     LHGAEMIFIPSATSRGLSMHLWDIEQRSHAIANGVFIGTINRVGKEEEFGPNDYYGTS
                     YFCDPRGQYINDALTGQPKASDKAEQLIICDMNMDLVQEVRNTWQFYRDRRPEMYGDI
                     VAA"
BASE COUNT         1555 a         1813 c         1818 g         1472 t
ORIGIN      
        1 ccacatacac atcggccaca taatcatccg tggcggtaat cacgcgtccg ttcttgatta
       61 acagggtact catacagcct ccttggagaa tggattttaa tgcctgttcg cactattcaa
      121 cgaagagcca actcgtctac aagactattt ccgttcgtat ttgcccatca actgcgcctg
      181 tcccacaatg tgactttcaa ttgctgcctc aacttgattg cgggaagcgc cgtttttcaa
      241 atggagcgaa ctgatattga gtgcgtacaa ggtgaagaaa tatcgatgag cgccacggcc
      301 gatcggcggg caggggcctt gataattcga ttgaccaaag ctgttggtgc cgcttaaacc
      361 gattgttgat tcggcctcga ggagtattga tgaagcaaca ggaatatcaa aaagcagcca
      421 gtgagtaaaa gttccgccgg gcgcatccgg atcatccata atcaaaacga aactctgtgt
      481 ggccgacggc gcgctgctcc aactcaactg cggcgatcga ttcgagccgg tgcaggtgat
      541 atctttcggg acgagatcgc ccatcgagaa agccggactg ctaagagtga acgtcactgc
      601 cgtgctcccc ggatcgatct gcggcgcatt cgatccacac gccgcaagtg tgacagccag
      661 actgataaaa ctgatcgatc tgtacgacat gaattgtagc atgggccatc gatcggacgc
      721 aagtttgccg cttgcgttaa gaactttttt tcagcgtgtt cgccatccct tttaccggtt
      781 gagaaggagt tgtcgctggc gctgtttccg ttgttggggg cggagtagaa tccattgaag
      841 gttgtacgat caaagagttg aacgcatcga cgtagtctcg gatcaaacct gcatacaact
      901 ccacttgacc tgaaccattg tatcgggttg caaaagtggc atagtctttc tgctgcatcg
      961 cggtaacttt caccgaatcg tttacgacaa aattgaagaa gccaatcaat tgattgcgtg
     1021 gatcggtgct gaatgaatcg aacatcgccg ggatcgaact gtaaccgcac gccgcaaagt
     1081 tcgatccgag aatttgcacc aagcccatac tgatcgatga gagtgcaagg ctgtcgttca
     1141 ggctgcgtgc cacgcccaac gctgcatact cgctatcttg attggcatgc acgttgagcc
     1201 acgggctgtt ggtgttcggt cgatactggt gtccttgcca attgacggtc gcatcaaatt
     1261 gaaagagttg gttgtaaacg tcggcgttct ctttacccca cctgaaccaa aataagtgat
     1321 tctcaaatcg gatcgtcatg cgtcccgccg cgccgctggc ctggccgccg gattcggtgg
     1381 cgatgacggc cacagcaatg ccggaatcga tgttgatctt ctgcgaaagg gtttcgatca
     1441 atccgccgta catgttccag attttgctgg cgacaactgc tttgaagccg gcattggcag
     1501 gtgtagttcg ttgtgaattt ggcgtcagca ctgcattgat aaagtcggct ggattgaaaa
     1561 ttgccccaaa cgaagcagtc ggctgctgac cgggggcgag cacatcggtg aattgcgctg
     1621 aggcatacac gcggccaaca aaccaggtat ccaataggct gatcaattcc aaactctgcc
     1681 cgctgttcag aatgccgacg acgttgcccg gatcgttggc gctgttgatc gatcgtagat
     1741 tgaccgctga gccgccgagc ggagtgaaag attttccgtc cgccgatagt gcagcaaact
     1801 gagcgacaac gtaaacgcgg caagtatgcc aggctgtgcc ttgtagatcg agttcaagtc
     1861 gcacgccttc tttgaggccg ccgactttgg tcgccggatc gatcagcgcc tggctgcgca
     1921 gggtcacgcc gccggagccg gctttgggtt gaacgaaaat tggatcggcc atgtttactc
     1981 tcctgtggag gctcccagcc aggttgtctt gttcgttaac gttttgagca ggagaacact
     2041 gagaacggcg gaatcaatcg tcattgatta aggtttgcct tgtaaaagaa ttttcgatag
     2101 cataccgcta aatcgatact cgcacaatct cgactgaaag cgaacaccaa gtcgctgaga
     2161 gtgatcaatc gatctcaatc atgatcgctt ggcgtttctt gagaagtctc actcaggccg
     2221 gtgtatcctg tcggatcgag ggtgcaggtt tcagcaagcg tgagtggaac catcgacgcg
     2281 agggcggctt ggtctgcgaa tcattctcat taccgcgccg ccgcgcgcgt agccacacgg
     2341 taagatacag ggccaagccg aagttaaaga aacttgtgcc acgcagatcc ggcaccgagc
     2401 cgatgaagga attgctcggc cagaattgtg ccacaaacgc ggcgaactgc ggaataatga
     2461 tctgatcgta accgatgatc aacatggcgc ccaagactgc gcccggtatg ctgcccgcgc
     2521 cgccaaggat caccatcgag agtgccagtg ccgagacatg gaatgcaacc atatccggtg
     2581 aaacaaacgc gaaggtactg gcatacaacg cgcccgctac ccccgcaatg actgaactca
     2641 cgatcagggc cagcgttttg tatcgatcga cattcacgcc ggtggatgcg gcggccgttt
     2701 catcatcgct ggccgcgatc cacgcgcggc cggtgcgcga tcgaatcaga cgctgggcga
     2761 ataaagccac aatcaacaca accacgaaaa caaaatagta ttgctgggtc tggccctcga
     2821 tcgggactga caggattcgc ggtggtggca gcgcgccgat accgccggcg ccgcccgtca
     2881 gatcgcgcag gttgttgatc acgcgttcgg ctaacagccc caacgccaaa gtcgcaacgg
     2941 caaaataatc actatgcaaa cgccgcgcca ggccgccttt gaccgcgcca tataatccgg
     3001 cgaatatcgc gctgattaag atcaagatcg tgaaatcaat cggctgaggg atcagcctgc
     3061 cgatcaaacc atatgggttg gcgagcaagg cgacggtata accgccgatg ccgaaactca
     3121 ccgcaaagcc caaatccaaa atgcccgcga cacctaacaa catgtttaag cccaaggcga
     3181 tcaatgcgta gatgctgacg atgcgcacaa gcgattgcca acccaaaccg gtgatcgatg
     3241 acacgatcgg gaacagcgcg agcccgagga agatcgcaat caaccagcgg ttcgatcgct
     3301 gcttgcccgg cgcggtcagg atcaccgaat cgcgaatcgt ttgcgatgcg gcctcttcat
     3361 tgctttcctc ggcggcgagg ccggttggcc gccagcacaa aacggcgatg aataagatca
     3421 gcaacaagac tggcgtccac tgtgcgctta aaaaataatc gctaaaagcc gaaaagatgc
     3481 cgagacccat cgaagccacc agcgcgccga tcgggctgcc gacaccgccc aagatcgcgg
     3541 cggcgaaagc catcaagccg ctctcagcgc cggccgcgcc aaacggtcga gtgtaataca
     3601 tcgcgaagat gaacgcggcg acaccggcta acgcgccacc gaacgcgaag gcgcgccgga
     3661 tcgcgctatt cagattcaca ccattgatct gtgccaattg tgggttttgc gatgcggctc
     3721 gaatcgcgcg acccgttttt gatcgtttca aatacagact ggcaccaatc gcgccggcga
     3781 cggctacaac taaaataaat aaattggcga agttgaaatt gacagttgaa tcgatgccga
     3841 gtgcgtgaac gaggttgatg ttgggcaaaa gagtaggaat gccgtctgtg ggcacttcat
     3901 tcaagcccgg cacactgcga tgttcgtgcc acacccaact gggcatgagc gtgcgccaga
     3961 tcatcgccac ttgaaacaac atgaatgaaa tgccgagcgt ggcgatcagt ggcgcgagac
     4021 gcgatcgacc gcgaaagggt ttgaaggcga tgcgttcgat cgccacgctg agtccggtgc
     4081 cgaacaatat gccgacgcct aatgtaatcg ccagtccgcc gatcaagacc agcgccgatg
     4141 aatcgggctg caagccaagt gcgcggacga gtgtcgtgat gacgaccgtg gtaagggcaa
     4201 agacatcgcc atgcgccagg ttgagtgtgc gcaccgtgcc atagatcacg gtgatgctga
     4261 tcgcattcag cgcgaggacg gcgccgttcg atattccgta gattaggatt tgcagtaaga
     4321 catcaccgca attggtgttg gccgcgcaga ccacagtgct cccccgttac tgatggacta
     4381 gattgtatcg cgcctactgt gccttagcaa cgataaagat ataccggaga tcgaccgtgc
     4441 cggtgttgat cacatcatgc tcggtctgtg gtggcacgta agcgatcagg cctgtagata
     4501 gatcgatcgg cttatgatcg gctaagcgga gttgtccgca gcccgctaac ggcacgatca
     4561 cttcttcgtt ggattgcgtg ctatgtaacc cgatcgattt accgggcgac aatgtgacca
     4621 gccccgattt gatcgatcgc gtttcgggcg ggccgccgag caacttgaag taagactgat
     4681 cgccaggatc gagttgaata actttagggc gtaacagagt gatacttgaa ttagccacaa
     4741 atacttcctg ataaaaatcg gagtcgaggc ttgcccatat cctgagcgca aagcgctcag
     4801 gtgggccgct aggcgtagcc ggcgtgaatc aggttgacct gctaaagtct cgactccctt
     4861 gggttgtaaa acgtgatcgc ttacctgcgc cttcgcttgg ccgcaacttt cttcttagcg
     4921 gttggtattg ctttcggctt gacgacgcgt accgacattt tattgaagtg tggaatgacg
     4981 tacttgctgt atgcttgaac ttgctcttct tcatcgccgc acatcaagta gatattgaat
     5041 tgatggacgc cgaggtcgcg cagttcttcc aatttgcgga tatgctcgct gatcggtccg
     5101 acgatgtcgt agcgatcgac gatctcatcc gtgacgaatt cggcgttact gctgcccact
     5161 tcagcgtggt gcagataatt gtagcccttg cgatcgcgca cataggctgt taattcttgc
     5221 ggcagctgcg ccggatcaca tttgctgatc agatcgacga cgtgattgga gactaatgcc
     5281 gggaaccaac gcacgtgatc gcgcgccact ttcatgtcgt cgctgatcca gatcggcgcg
     5341 gcggacatga tcttgatctt ggagaagtcg cgtccggatt gatccgcgcc cgctttgacc
     5401 atttccatgc accatttgat caaatgcgga tcggcaaatt gtaagatgat ccgatcggcc
     5461 acgcggccgg ccatgcccaa ggccttcggt ccatagccgg caacccagac gggcggcaca
     5521 cccccatcgg cccacgttaa ttgtatcggc tgcttttcga aggtgatctg tttgcccgcc
     5581 gtgagatcgc ggaactcttg gatcgattgt tccagaattt cgacggtggt cggcttcttg
     5641 cccatcaccc gccgcgagga atcgcctcgg ccaataccga gctgcatccg tccgccggta
     5701 aagcgctgca gcgtcgcgaa ggtactcgcc gtcacggtga tgtcgcgcac acgcggattt
     5761 gtcacgcacg ggccgagttt catctttttg gtgttcatgc ccagcaccgt cagctgcatg
     5821 tacacttcct gccacagcac gtgcgaatca aatgcccaga tataactgaa gccgcccgct
     5881 tccgcagctt gcgcgagttt gatcgcgcgc ggtatgggga tatcggactt gaaagtgatc
     5941 ccaaagtcca ttgtctgcct ccagtaagaa gtcggcgttc cagtagtaat actaggctgc
     6001 tacgatgtcg ccatacatct ccgggcgtcg atcgcgatag aactgccacg tgttgcggac
     6061 ttcctgtacc agatccatat tcatatcgca gatgatcagc tgctctgcct tatcgctggc
     6121 tttgggttgg ccggtgagcg catcgttgat gtactgtccg cgcggatcgc agaaatacga
     6181 tgtgccgtaa taatcgttcg ggccgaactc ttcttctttg cccacgcgat tgatcgtgcc
     6241 gatgaacaca ccgttcgcaa tcgcgtgcga tcgctgttca atgtcccaca aatgcatcga
     6301 taagccgcgc gaagttgccg acggaatgaa gatcatctca gcgccatgca agcctaacat
     6361 gcgcgggcct tccgggaaat gtcgatcgta gcaaatgtac acgccgactt tgccgaccgc
     6421 agtatcaaaa accggatagc ccagattgcc cggtcggaag tagaacttct cccagaaacc
     6481 attgacctgc ggaatgtgcg tcttgcgata cttgcctaga aacttgccat ccgcatcgat
     6541 tactgcagcg gtgttgtagt acatgccggt ctgctcttct tcgtacatcg gcacgatcaa
     6601 caccatcttt aatttcttag cgaggtcttg cgccaacttg gtggtaggtc cgtcgggc
//
LOCUS       DASRTJ010000395         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_327332,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000395 DASRTJ010000000
VERSION     DASRTJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327332"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFF70_07940"
     CDS             complement(<1..858)
                     /locus_tag="VFF70_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZY44663.1"
                     /translation="MAKYAAAVDQGTTSTRFMIFDHAGKVVEVDQREHEQIYPKPGWV
                     EHNPMEIWLRTQEVIKEALAKSKVDPKDIAAVGVTNQRETTVVWEKATGKPVYNAIVW
                     QDTRTDQICNDLAKDGGQDRFRLKVGLPLATYFSGPKIKWILDNVPGARAKAEKGEVL
                     FGNIDTWVIWNLTGGPNGGVHVTDVSNASRTMLMNLDTLDWDPDMLKVMGIPRAMLPA
                     VKASSEVYGKAVGDLAGIPVAGDLGDQQAALFGQTCFSAGEAKNTYGTGCFMLLNTGN
                     KAVQSKNGLL"
     gene            complement(920..1552)
                     /gene="dhaL"
                     /locus_tag="VFF70_07945"
     CDS             complement(920..1552)
                     /gene="dhaL"
                     /locus_tag="VFF70_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HZY44664.1"
                     /translation="MAVTRDDVITWLNNCGQVITENKDYLTQLDSDIGDADHGANMDR
                     GFKAALAKLPSVADKDIGTLFKSIGMTLVSTVGGAGGPLYGTFFMQAGAASANKLELS
                     AADWAAALESAINGVIARGKANPGDKTMIDALLPALNALKQAVADGVDMGDALRRSAA
                     AAEQGMKDTIPLVAKKGRASYLGERSAGHQDPGATSSYLILKTAAETWGR"
     gene            complement(1554..>2238)
                     /locus_tag="VFF70_07950"
     CDS             complement(1554..>2238)
                     /locus_tag="VFF70_07950"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HZY44665.1"
                     /translation="YTGDIMNFEMAAELAKAEGIEVEAVITNDDVAVQDSLYTAGRRG
                     VGVTVLAEKICGAAAQAGRSLKEVAEMCRKVNGQGRTMGMALTSCTVPHAGKPTFDLP
                     EDQMEIGIGIHGEPGRTRMALKSADEITEMLMEAIIGDLPYKSGDNVLLFVNGLGGTP
                     LIELYIVYRKAYEIATKKGLKVVRNLIGPYITSLEMAGCSITLVKMDDDMIKLWDAPV
                     KTPGMRWGV"
BASE COUNT          444 a          678 c          590 g          526 t
ORIGIN      
        1 caacaggccg ttcttcgatt gaacagcttt gttgccggta ttgagcagca tgaagcagcc
       61 ggtgccgtaa gtattcttgg cctcgcccgc actgaagcag gtttgaccaa acaaagccgc
      121 ttgctgatcg cctaagtcgc cggcgacggg gatgccagcc agatcgccta cagccttgcc
      181 atatacttca gaggaagctt tgactgcggg cagcattgcg cgcggaatgc ccatgacctt
      241 gagcatatcc ggatcccaat cgagcgtgtc gagattcatc agcatcgtgc gggaagcatt
      301 cgagacatca gtgacgtgca caccgccgtt aggaccgccg gtcagattcc agatgaccca
      361 ggtatcgatg ttgccgaaca ggacttcgcc cttctcagct ttggcgcggg cgccgggtac
      421 attgtcgaga atccacttga tctttgggcc ggagaagtat gtggctaacg gcaggccgac
      481 tttcaggcgg aagcgatctt gtccgccatc tttcgccaga tcgttgcaga tctgatccgt
      541 gcgagtatcc tgccacacaa tcgcgttgta gactggtttg ccagtagctt tctcccatac
      601 caccgtcgtc tcgcgctgat tggtcacacc gactgcagcg atgtccttag gatcgacctt
      661 gctcttggcg agcgcctctt tgatcacctc ttgagtacgc agccaaatct ccatcgggtt
      721 gtgttccacc cagcccggct tcggataaat ctgttcgtgt tctctctgat caacttccac
      781 gactttgccc gcgtgatcga agatcatgaa gcgggtgctg gtcgtgcctt gatcaactgc
      841 tgctgcatat tttgccattt gaaacctcca ggtgaattaa atgtgcgacg cacgtctccg
      901 cacatcccac attgtttgac tatctgcccc aagtctcggc ggcagttttg agaatcaaat
      961 acgacgaagt ggcgccgggg tcttgatgtc cagcgctgcg ctcacccaag tatgatgcgc
     1021 gacctttttt ggcgaccagt ggaatggtgt ctttcatgcc ctgttcggca gcggcggccg
     1081 atcggcgcag ggcatcaccc atatcgacgc catcggcgac agcttgcttc agcgcgttca
     1141 acgccggcag caacgcatcg atcatagtct tgtcgccggg gtttgctttg ccgcgggcga
     1201 tcacgccgtt gatagccgat tccaacgcag cggcccaatc ggcagccgat agttccagct
     1261 tgttcgcgct ggccgcaccg gcctgcatga agaacgtgcc gtacaacggg ccgcctgcgc
     1321 cgccaactgt cgagaccagc gtcatgccga tcgacttgaa caacgtgcca atgtctttat
     1381 cggcgaccga tggcagcttg gccagtgcgg ctttgaaacc gcgatccatg ttggcgccgt
     1441 gatcggcatc gccgatgtcg gagtccagct gcgtgagata gtctttgttc tcagtaatga
     1501 cctgaccaca gttgttcagc caagtgatca catcatctcg ggtgacagcc atcttatact
     1561 ccccaacgca taccgggcgt cttcaccggc gcatcccaca acttgatcat atcatcgtcc
     1621 atcttcacca gcgtgatcga gcagccggcc atctccagcg atgtgatgta cggcccgatc
     1681 agattgcgca cgaccttgag acctttcttc gtggcgatct cgtaggcttt gcgatagacg
     1741 atatacaact cgatcaacgg cgtaccgccc agtccattga cgaacagcaa cacattgtca
     1801 ccagacttgt acggcaaatc accgatgatc gcttccatca gcatttcggt aatctcatcg
     1861 gcggacttta aggccatgcg cgtgcggcct ggttcgccat gaattccgat gccgatctcc
     1921 atttgatctt ccggtagatc gaacgtgggc ttgcccgcat gcggcaccgt gcaggaagtc
     1981 agcgccatac ccattgtgcg gccttgccca ttgaccttgc ggcacatttc ggccacttct
     2041 ttgagtgagc ggcccgcttg cgcggccgcg ccgcagatct tctcggcaag aacggttacg
     2101 cccacgccac gtcgaccggc agtgtacaaa ctatcttgca ccgccacatc gtcattggtg
     2161 attacggctt cgacttcgat gccttcggcc ttcgccaatt cggcagccat ctcaaagttc
     2221 atgatgtcgc ccgtgtag
//
LOCUS       DASRTJ010000396         7072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_275517,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000396 DASRTJ010000000
VERSION     DASRTJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275517"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFF70_07955"
     CDS             complement(<1..674)
                     /locus_tag="VFF70_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZY44666.1"
                     /translation="MPQTELFEAIPRDPSLVSRVVEQIEGLIIEGRLRPGDQIPPENE
                     LARQLGVSRTVIREAMRTLIAKNLLQVQAGSGTVVSTPSGKSLAQSMAMLLRNDNSEL
                     DIDKVVQVRRLLEIEIAGLAAERRTAEDLARIEETLQQMAGTRDRESFATSDVDFHTA
                     LAITTHNELFALLLDSISDVMLEIRRTGYDVPGTPERGLKYHRAIFDQIKAGNPAGAR
                     QAMREH"
     gene            complement(737..1507)
                     /locus_tag="VFF70_07960"
     CDS             complement(737..1507)
                     /locus_tag="VFF70_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZY44667.1"
                     /translation="MTNESPFRTVGNKGRLVDRVVNDLQRLIVDGALEPGMKLPPERE
                     IAEQLDVSRTVVREAVRILAASGLLVARRGVGTIVQQITQEHIGERLNLLLRTSGISL
                     AKLHEVRSILEVEMAGLAAQQATGDDLANLRGILTEMDETKESPEGFADQDAEFHTTL
                     AQTTHNPLYSVLLGSIRDLMQEVRLSVSHNPDLFATVMPDHYRILDRIIAKDVDGARQ
                     TMQAHLEHARSIQQAFLAQQESAKAAEVAEPAGADQPA"
     gene            complement(1528..2736)
                     /locus_tag="VFF70_07965"
     CDS             complement(1528..2736)
                     /locus_tag="VFF70_07965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZY44668.1"
                     /translation="MATELFIHKMSEHMESAKIIRWLVQEGDPIRQFQIIMEVETDKV
                     AAEIEAPASGILKGVRAGAVDGADVPVGETIAFIAGPDEVVPVLPPLGSVSSANPIKL
                     PPASPSPSTNVEPGPIKATPVARRVAKDLGVDLSLVKGSGPDGRIKDEDVRAYAAAAK
                     TRSAGPAPAPLAAAGEWLDLTAAQRVTGQRMLESVQTAPQFALTLNTDMTQVLWLREA
                     LMDRVLVETGERLSITAILVKVVAAALQLHRRANASFEDGKIKLYQAINIGVASGNEA
                     GLVVPVIKAADQKSLAQIVRDIKVFQDKTQQMRFGADDLTGGTFTISNLGMYGIEQFN
                     AILNPPQSAILAVGKVIKTPIGLPDDSIAVHPMMSLTLTIDHRVLDGMQGAQFLSDLK
                     ARLEKPFFLL"
     gene            complement(2760..3782)
                     /locus_tag="VFF70_07970"
     CDS             complement(2760..3782)
                     /locus_tag="VFF70_07970"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZY44669.1"
                     /translation="MSADLIMQVPAGTPAKEMSYQEAIREALREEMVRDERVFLMGED
                     IAKHGGAFGVTRTLFDQFGPERVRNTPISENTIVGAGTGAALVGMRPVMEIMFSDFSG
                     LAMDQICTQAAKLRFMTGGQCTIPWVLRMPTGGGAGKSIAAQHSQSLEVWYASIPGLK
                     VVLPSTPYDAKGLLKAAIRDDNPVIFLEHKFLYSFRGLVLETDYVLPLSQCDIKRAGK
                     DVTVVCSGSLVWYAMAAAKMLEPQGIDVEVVDVRTISPLDEETIGRSARKTGRVVVAA
                     EACRSYGPTGEWGMVVMEQAFDYLQAPIVRVTGRNSPIPFADSIEKGVWPETEDVVRA
                     IQQVMA"
     gene            complement(3853..4812)
                     /locus_tag="VFF70_07975"
     CDS             complement(3853..4812)
                     /locus_tag="VFF70_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZY44670.1"
                     /translation="MKISHEMLLEMYRRMVRIREFELAAMDLFKRGQVKGTVHPYIGQ
                     EASGVGVCMALGVEDLVAGTHRSHGHNLAKGANSGKMLAEILGKETGYCKGRGGSMHI
                     AAFETGSLGALAVVASGIPLAVGAALAFKLRGENRVAVPFTGEAGSNTGNWHESLNMA
                     SIWDLPVVFVLENNHYGVSTNIKDTAHIEDLSIRAKAYGIPGVRVDGFDVLAVYEAAV
                     EAVARARRGEGPTLLVTESYRIEGHYAGEPEVYRSRAEVEEYRKREPIGRFRHYLLEN
                     SQATGAELDGIDAAIKQEMIDAVQFAKDSPAPNPATALDYIYA"
     gene            5161..5283
                     /locus_tag="VFF70_07980"
     CDS             5161..5283
                     /locus_tag="VFF70_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44671.1"
                     /translation="MDQLHKRTTAEQVKVLLHDYCQGTMSRAEAEEMLQIRKAL"
     gene            5413..5535
                     /locus_tag="VFF70_07985"
     CDS             5413..5535
                     /locus_tag="VFF70_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44672.1"
                     /translation="MIALIGIIQSFQVKIMVGGAPLHSHRTSAAVRKTKELLAA"
     gene            5619..6473
                     /locus_tag="VFF70_07990"
     CDS             5619..6473
                     /locus_tag="VFF70_07990"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZY44673.1"
                     /translation="MQTILRSETIEVKIRTDGPFIVIGEKINPTCRKKLAEALLAGNF
                     DYVRDLAKKQVEAGADVLDLNVGVPGLDEVELLPQIVSVVATTVRVPICIDSPNPSAL
                     AAALRVAPGKPLVNSVNGEKKSLATILPIVKDRGAAVIALTMDDNGIPNNAEDRLTIA
                     ARIIERAASIGIPIEDVVIDPLVMSVGADQHAGAITLRTIELVRAEFGANINLGASNV
                     SFGLPDRHTLNQAFLALAIGAGATCAITDPLKLTATIRAADLLRGRDLYAKRYISYCR
                     AHPKETAP"
     gene            6491..>7072
                     /locus_tag="VFF70_07995"
     CDS             6491..>7072
                     /locus_tag="VFF70_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44674.1"
                     /translation="MITESIVLRDWPLNIDVDMVLRGQGADARVIRQRRPQLIDIAQR
                     AIEDGQQLIKPAVVYRRVPVASLHHENLVLKNGAVLKGELLALHLAPAEQIILLVCTI
                     GPALGQYVAEVLTTDPAYGLALDSFGSMAVEALSVAACTFFEDQARQRSLFTSVPLSP
                     GMIGWPVDKGQPQIFSLLAADLIGVTLNEYALMI"
BASE COUNT         1547 a         2033 c         1906 g         1586 t
ORIGIN      
        1 aaatgctcac gcatggcctg ccgcgcccct gccggattgc ccgctttgat ttgatcgaag
       61 atcgcacggt gatatttgag accacgttcg ggtgtgccag gaacgtcgta gcccgtccgg
      121 cgaatctcta gcatcacgtc agagattgaa tcgagcagca aggcgaagag ttcgttatgc
      181 gtggttatcg ctaaggcggt atgaaaatca acatcgctgg tggcgaagct ctcgcgatcg
      241 cgggtgcctg ccatctgctg caaggtttct tcaattcgcg ccaggtcttc agccgtgcgt
      301 cgttccgcag ccagtccagc gatttcgatt tccaggagac gccgaacctg cacaactttg
      361 tcgatgtcca actccgagtt gtcattgcgc aacagcatgg ccatcgattg cgccagagat
      421 ttgccgctgg gtgtactgac gaccgtaccg ctgcccgctt gaacttgaag caggttcttg
      481 gcgatgagcg tgcgcatggc ctctcgaatg acggtccggc tcacgcccaa ttgccgggca
      541 agttcgttct cgggtggaat ttgatcgccc ggtcgcaatc ggccctcgat gatgagacct
      601 tcaatctgct caaccacgcg gctgaccagc gacggatcgc gcgggattgc ttcaaacaac
      661 tccgtctgtg gcatggaact ctgatcgtcg aatgttctca tatcgcacgc ttgatcgtcg
      721 gttacacttc aacggctcag gcaggctgat ccgcacccgc cggctctgcc acttcagcag
      781 ccttggcact ttcttgttga gccaggaatg cctgctgaat actgcgagca tgctccaggt
      841 gcgcttgcat tgtctgacgg gcaccgtcca cgtctttggc gatgatccga tccagaatgc
      901 gatagtgatc gggcataacc gtcgcgaata aatctggatt gtgggagact gagagtcgca
      961 cttcttgcat gagatcccga atcgagccta acaacacgct atacaatgga ttgtgcgtgg
     1021 tttgggctag cgtggtgtga aattcagcgt cctggtcggc aaatccttca gggctttctt
     1081 tagtctcatc catttcggtg aggatgccgc gcaggttggc tagatcgtcg ccggtcgctt
     1141 gctgcgcggc gaggccggcc atctcgactt caaggatcga gcgcacttca tgcagcttgg
     1201 ccagtgaaat gccgctcgtc cgcagcaaca aattcaatcg ctcgccgata tgctcctgag
     1261 tgatctgctg cacgatcgtg cccaccccac gccgggcgac taacaatccg cttgccgcca
     1321 ggatgcggac agcttcgcgc acgaccgtcc ggctgacgtc gagctgttcg gcgatttccc
     1381 gttcgggcgg cagtttcatg cccggttcaa gagcgccgtc gacaatcaaa cgttgtagat
     1441 cgttgaccac tcgatcgacc aaccggccct tattaccaac agtgcgaaat ggactttcgt
     1501 tcgtcatgct catccgcttg atcacaccta cagcaaaaag aacggctttt cgagccgcgc
     1561 tttcaaatcg ctcaggaatt gcgcaccttg cattccatcc agcacccgat gatcaatcgt
     1621 caaggttaag ctcatcatcg ggtgcacagc gatcgagtcg tcgggcaagc cgatcggggt
     1681 cttgatcact ttgccgacgg ccagaatcgc gctttgcggc ggattgagga tggcgttgaa
     1741 ctgctcgatg ccgtacattc ccaaattgga aatcgtgaaa gttccccccg ttagatcatc
     1801 cgcgccaaag cgcatctgct gcgtcttgtc ttgaaacact ttgatatcac ggacgatctg
     1861 cgccaacgat ttttgatcgg cggctttgat gaccggcacc accaggcctg cttcattgcc
     1921 ggacgctacg ccaatgttaa tcgcttggta cagtttgatc tttccatcct caaacgaagc
     1981 gttcgcgcgc cgatgcagtt gcagcgcagc cgctacaacc ttgaccagaa tcgcggtgat
     2041 cgataagcgc tcgcctgtct caaccagcac acgatccatc aacgcttcac gcagccacaa
     2101 gacctgcgtc atatcggtgt ttagggtgag ggcaaattgc ggtgcggtct gaacactctc
     2161 gagcatgcgc tgacctgtga cgcgctgtgc agcggtcaag tccagccatt cccctgcagc
     2221 cgcgagcggc gcaggggccg gcccagccga tcgagtcttg gcagccgccg cgtaggcgcg
     2281 cacatcttcg tctttgatgc ggccatcggg gccggaacct ttcaccaggc tcaaatcaac
     2341 gcctaagtct ttagcgacac ggcgcgcgac tggcgtggct ttgatcgggc ccggctcgac
     2401 gtttgtagat ggagatggcg acgcaggtgg cagcttgatc gggtttgctg agctaacaga
     2461 tcctaaaggc ggaagtactg gcacgacttc atcaggtccg gcaatgaagg caatcgtttc
     2521 accgaccggc acatcggcgc catcgaccgc gcccgcgcgc acgcccttca aaatgcctga
     2581 agccggcgct tcgatttccg ctgcaacctt atcggtctcc acttccatga tgatctggaa
     2641 ttgccgaatt ggatcgcctt cctgaaccag ccaacgaatg atcttggccg actccatgtg
     2701 ctccgacatt ttgtgaatga ataattcagt tgccatccgt ctctgttccc gcaccctgcc
     2761 tatgccatga cttgctgaat tgcccgcacg acgtcttcgg tctcaggcca cacgcctttt
     2821 tcaatgctat cggcaaaggg gatcggcgaa ttgcggcccg tcactcgcac aatcggcgct
     2881 tgcagataat cgaaggcttg ctccatcaca accatgcccc actcacccgt cggtccatag
     2941 gatcgacacg cttcggcggc caccaccacc cgaccggtct tccgcgctga tcggccaatc
     3001 gtttcttcat ctaatgggct gatcgtacgc acgtcgacga cttccacgtc aataccttgc
     3061 ggttcaagca tcttggccgc cgccatggca taccagacca gcgaaccaga acagacaacg
     3121 gtgacatctt tgcccgcacg cttgatgtca cactggctga gtggcagcac gtagtccgtt
     3181 tctagcacta gaccgcgaaa agaatagagg aatttgtgct caaggaagat caccggattg
     3241 tcgtcacgaa tggcggcctt gagcaggccc tttgcatcgt agggtgtgga gggcaaaaca
     3301 actttgagtc cgggaatact ggcataccaa acttcgaggc tctgcgagtg ttgggccgca
     3361 atgctcttgc ctgcgccgcc gccggtcggc atgcgcagta cccacgggat agtgcattgg
     3421 ccgccggtca tgaagcgcag cttagcggcc tgcgtgcaga tctgatccat cgccaggcca
     3481 gaaaagtccg agaacatgat ctccatcacg ggtcgcatgc ccaccaaggc cgcgccggtg
     3541 ccggccccga cgatcgtatt ctcgctgatc ggagtgttgc gcacgcgctc cggcccaaat
     3601 tgatcgaata gagttcgcgt gacgccaaat gcgccaccat gcttggcgat gtcttcgccc
     3661 attaaaaaga cccgctcgtc gcgcaccatt tcttcacgga gtgcttcgcg gatcgcttcc
     3721 tgataggaca tttcttttgc cggcgttccg gccgggacct gcatgatcaa gtcggcagac
     3781 attgtcggtt tacttgatcc gagcgatacc gcatctcgtt gtgctgtgcc catgctgatt
     3841 ccctcaatga aattaagcgt agatgtaatc cagcgcggtt gccggattgg gcgccgggct
     3901 gtctttggca aactgcaccg catcgatcat ttcctgtttg atcgcggcgt cgatgccgtc
     3961 gagttcagcc ccggttgcct gactgttttc aagcaggtaa tgacggaagc gtccgatcgg
     4021 ctcacgcttg cggtactctt ccacttccgc ccgcgaacga tagacttccg gctcaccggc
     4081 gtagtggcct tcaatacgat agctctctgt gacaagcagt gttggtccct cgccgcgtcg
     4141 cgcgcgggcc acggcttcga ccgctgcctc gtacacggcc agcacatcaa agccatcgac
     4201 gcgcacgccc ggaatgccat aggccttcgc gcggatggac aggtcttcaa tgtgggcggt
     4261 atccttgatg ttagtggaca caccatagtg gttattctct aaaacaaaaa caactggcaa
     4321 atcccagatt gaagccatgt tcagggattc atgccagttg cctgtgttcg atccggcctc
     4381 gcccgtgaag ggcacggcca cccgattttc tccacgcagc ttgaaggcca aggccgcgcc
     4441 gactgccaat ggaattccag aagcgacgac ggccaatgcc cccaagctac ccgtctcaaa
     4501 agccgcaatg tgcatcgagc caccgcgtcc tttgcaataa ccagtctcct tgcccaagat
     4561 ctcagccagc atctttccag aattagcacc cttggccagg ttatgcccat ggctgcgatg
     4621 cgtgccggcc accaaatctt ctacacctag cgccatgcac actcccacgc ctgacgcctc
     4681 ctggccgatg tacgggtgca ccgtaccctt tacttgacca cgtttgaaca ggtccatggc
     4741 ggccaactca aattcacgaa tgcggaccat acggcgatac atctcaagca gcatttcgtg
     4801 tgagatcttc ataagagagg tgttctccta aaaaggcaaa aatttctcaa gtaaatttgt
     4861 aatacatcat acctcatact gcattccata atataacaat tatttatgcg ttgtcaagga
     4921 ccaattttgt attttacgga acataagtaa cctgagttcg ggttaagccg atgttatacg
     4981 cggcaaggca tccaaaatgt aaggatggtt tcttcggctg atggcaataa gcgatcaaac
     5041 caataagtaa attaatcgag aagttcacga cgctgtgtta taaaacttga aaagcgaact
     5101 gctctaaagc gccagaagcc gaacgatcct aatagactgc tcgctaaaac aaggagcacc
     5161 atggatcaat tgcataagcg caccactgcc gagcaagtca aggttttgct gcacgattat
     5221 tgccaaggca ccatgagccg ggctgaggcc gaagagatgt tgcagatcag gaaggcactt
     5281 tagtacccaa accgatcgag cgcaaaatgt ggagagttat cactctgaaa tgggatgatt
     5341 tgacatagac actgtcggca agttgatgta ctcttactaa atcatgcagt cggccctatc
     5401 atcggtgaat cgatgattgc attgatagga ataattcaat cgtttcaggt caagatcatg
     5461 gtcggcggcg caccgctgca ctcgcatcgg acgagtgcag cggtgcgcaa gaccaaagaa
     5521 ttattagcgg cttagcattt cctcgctaaa ctgagcaggc ggtatactct aggcattgag
     5581 gcgtgcgatg aagatttttt taagcaggac actgattcat gcaaacgata ttacgcagtg
     5641 aaacgatcga agtcaagatc agaaccgatg gaccttttat cgtcatcggg gagaagatta
     5701 atccgacatg ccgcaagaaa ctggctgagg cgctgttggc tggtaacttc gactacgttc
     5761 gagacttggc gaaaaaacag gtagaggccg gtgcagatgt gctggacttg aatgtcggcg
     5821 tgccgggcct agacgaggtc gagctattac cgcagatcgt gagcgtggtg gcaaccaccg
     5881 tccgggtacc aatttgcatc gactccccga acccgtcggc cctggcggcg gcgttaaggg
     5941 tcgcacccgg taagccgctg gtcaactccg tgaacggcga aaaaaaatcg ctcgccacta
     6001 ttttaccgat cgtcaaagat cgcggtgccg ccgtgatcgc attgaccatg gatgacaacg
     6061 gcatccctaa caacgctgag gaccgcttga cgattgcggc caggatcatc gagcgggcgg
     6121 cctcgatcgg catcccgatc gaagacgtcg tgatcgatcc attggtcatg tcggtcggcg
     6181 ccgatcaaca tgcgggcgcg atcaccctgc gaacgatcga actcgtccgt gcagaattcg
     6241 gtgcaaatat caatctgggc gcgagcaatg tctcgttcgg tttgcccgat cggcacactc
     6301 tgaatcaagc gtttctcgca ttggccattg gcgccggtgc cacgtgcgcc atcaccgatc
     6361 cgctgaagtt gaccgcgacc atccgggcag ccgatctgct gcgcggccgc gatttgtatg
     6421 cgaagcgcta tatcagctat tgtcgcgctc acccgaaaga aaccgcgccg tgaaccgaat
     6481 caggaatgag atgatcactg agtcgatcgt gctgcgtgat tggccgttga acattgatgt
     6541 ggatatggtg ctgcgcggac agggcgcgga tgcccgcgtg attcgtcagc gcagaccgca
     6601 attgatcgac attgcccagc gggcgattga ggacgggcaa caactaatca aacccgcggt
     6661 cgtctatcgt cgcgtgcccg tcgcatcttt gcatcacgag aatttagttt taaagaatgg
     6721 cgctgtactc aagggcgagc tgctggctct acacttggca ccggccgaac aaattatctt
     6781 gctggtgtgc acgatcggtc cggccttggg gcaatatgtg gcagaagtcc tcaccaccga
     6841 tccggcctac ggcttagcgc tggatagttt tggctcgatg gcggttgagg ccttgagtgt
     6901 cgcggcgtgc accttcttcg aagatcaggc caggcagcgc agcctgttca ccagcgttcc
     6961 gctcagtccg ggtatgatcg gctggccggt ggataaggga caaccccaga ttttcagcct
     7021 attggctgcc gacttgattg gcgtaaccct caacgagtat gcgctgatga tc
//
LOCUS       DASRTJ010000397         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_251755,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000397 DASRTJ010000000
VERSION     DASRTJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251755"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFF70_08000"
     CDS             complement(<1..346)
                     /locus_tag="VFF70_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44675.1"
                     /translation="MPTDTHNPWQIIQARAPVVDLHIHPAMNRLALKQNLNVRSVVSR
                     SFNPFAVRASWPRLKKGGYSAILSVLHVPERGLLKDFPIINLFRILRPDLWHRLLGNP
                     SFDATLRIMADLE"
     gene            540..1742
                     /gene="rocD"
                     /locus_tag="VFF70_08005"
     CDS             540..1742
                     /gene="rocD"
                     /locus_tag="VFF70_08005"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HZY44676.1"
                     /translation="MNSQDYIAIEEQYGAHNYHPLDVVIEKAEGAWVYDVEGKKYLDF
                     LSAYSAVNQGHNHPRIVKAMIDQLGKVALTSRAFRNDQLPLLAKELCELTGYEMMLPM
                     NSGAEAVETAIKAARKWGYKVKGIPDGLAEIISCGDNFHGRTITIITMSTEPQYKDGF
                     GPLTPGFKSIPYGDAKALEQAMTPNTAAFIVEPIQGEAGVHIPPDGYLKQVEAICKQN
                     RVLFIADEIQSGLGRTGKMFAHQWEDVKPDAVIIGKALSGGLYPVSAVVSSKEVLGVF
                     MPGDHGSTFGGNPLACAVAREALKVLVEENLFDRSAELGEYFKGKLQRLESQHIKEVR
                     GRGLFIGVELYPEAGGARRFCEALKDRGLLCKETHQDVIRFAPPLVVKKVDLDWAYGQ
                     IEDVLMNM"
     gene            complement(1906..>3161)
                     /locus_tag="VFF70_08010"
     CDS             complement(1906..>3161)
                     /locus_tag="VFF70_08010"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZY44677.1"
                     /translation="DQHRLDAIEQIHFTNTYTRSISMIVVNVPPAHDRARFDLRSIQL
                     NQAPGRVKLIGSVLTISLPSDLAPNASVDLTIDFTVYIPALADTQSFASSNLAYTDEA
                     TNIGYWYPILAPFRSGKWISVPWYPIGDPFASDIADYTATITATPGVTVVAGGLMSHQ
                     DNVWHYAIDRARTFGMLASSHYVETHYTFSGVTYSVYTFDRHTILAWPALYTLVRAAQ
                     LYGQLYGTYPYSTLRAAEVSGPWSMEFSGLVAIGSTDYAGYDDTTHNRLFRIIAHEVS
                     HQWWYNIVGDNQVQEPWLDEGFARFNELRFYETYSPQDAQWWWSTIIDPWTAEGPIDQ
                     PISSFDSQRVYLTDVYNQGAQFLDALRTRLGRSNFNLFMRDLFQREAFGLMTTNDFFS
                     VVREHTKVNLASLLRKYFSRKYWYK"
BASE COUNT          773 a          761 c          911 g          716 t
ORIGIN      
        1 gttcgagatc ggccatgatc ctcagcgttg catcgaacga tggattgccc aacaaccgat
       61 gccagagatc ggggcgcagg atgcgaaaca gattgatgat tggaaaatct ttaagcaagc
      121 cgcgttccgg cacatgcagc accgataaga tcgcactgta gccgcctttc ttcaagcgcg
      181 gccaactggc gcgcacggcg aaaggattga aactgcgtga gaccacagat cgcacattta
      241 ggttttgttt gagcgccagg cgattcatgg cgggatgaat gtgtagatcg acgaccggcg
      301 ccctggcttg aatgatctgc caagggttgt gagtatcggt aggcatgcga acttcctggg
      361 aagctatcag tgactgagac taggaacgac acttaatcaa gcgtgtggac attatacgat
      421 cacctgagtt gataagcaaa agctaggcgg ttgtcatttg gtatcagcgc ggactcgatt
      481 tctgtccata gacattggcc atgctataat gcgcttatct tcttcatcgc ggagcctgca
      541 tgaattccca agactacatt gcaattgaag aacaatatgg tgcgcacaac tatcatccgc
      601 tcgatgtcgt gatcgaaaag gctgaaggtg cgtgggttta tgatgtcgaa ggcaaaaaat
      661 atttggactt cctgtcagct tattcggccg tgaatcaagg ccacaatcat ccgcgcatcg
      721 tcaaggcaat gatcgatcag ttaggcaagg tcgcattgac gtcgcgtgca tttcggaatg
      781 atcagctgcc tttgctcgcg aaagaactgt gcgagctcac cggctacgaa atgatgctgc
      841 cgatgaactc cggcgcggag gcagttgaga cggcgatcaa agccgcgcgc aaatggggct
      901 acaaggttaa aggtatcccc gatggactgg cggagatcat ttcatgcggc gataacttcc
      961 acggtcgaac gattacgatc atcaccatgt cgaccgagcc gcaatacaaa gacggcttcg
     1021 ggccattaac acccggattc aaatcgatcc catatggcga tgccaaagcg ttggaacagg
     1081 caatgacgcc taataccgcg gcgttcatcg tcgaaccgat tcaaggtgaa gcgggtgtgc
     1141 atataccgcc cgatggttat ctcaagcaag tcgaagcgat ctgcaagcag aaccgcgtgc
     1201 tcttcatcgc cgacgagatc caatcgggtt tagggcgaac cggaaaaatg ttcgcgcatc
     1261 aatgggaaga cgtcaaaccc gatgcggtga ttatcggcaa agcgttgtcc ggcggactat
     1321 atccggttag cgcggtcgta tcgagcaaag aagtattggg tgtattcatg cctggcgatc
     1381 atggttcgac gttcggcggg aatccgctgg cctgcgcggt cgcgcgcgaa gcgttgaaag
     1441 ttttagtgga agaaaattta ttcgatcgat ctgccgagtt gggcgaatac ttcaagggta
     1501 agttacagcg cctcgaaagt cagcatatca aagaagtgcg tgggcgcggc ctgttcatcg
     1561 gcgtcgagtt gtatcccgaa gcgggcgggg cgcggcgctt ctgtgaggcg ttgaaagatc
     1621 gcggcttgtt gtgcaaagag acccatcaag atgtgatccg ttttgcgccg ccgctcgtcg
     1681 tgaagaaagt agacctcgat tgggcgtacg ggcagatcga ggatgtgttg atgaatatgt
     1741 gatgtaacga gtgataagtg ctgagtgaat agaagcgggt gacctccgaa gttttcaaaa
     1801 cttcggaagt ctttttgttc cacgagtcat tgcgagcgga gcgatgcaat ctcagtatcg
     1861 atcaagagat taccacgccg cccatggtgg ctcgccatga cggtctcact tataccaata
     1921 cttccgcgaa aaatattttc gcagcaatga cgctaaattc actttagtgt gctcgcgtac
     1981 cacgctaaag aaatcattgg tcgtcatcag gccgaatgct tcgcgctgaa agagatcgcg
     2041 cataaataaa ttgaagttgc tgcggccgag gcgggtgcgc agcgcgtcga ggaactgcgc
     2101 gccttgattg tagacatcgg tcaaatagac gcgctgtgaa tcaaagctgc tgatcggttg
     2161 atcgatcggc ccttcggctg tccatggatc gatgatcgtg ctccaccacc actgcgcgtc
     2221 ctgtggcgaa tacgtttcgt aaaaacgcag ttcattaaaa cgcgcaaagc cttcatccag
     2281 ccagggttct tgcacttgat tgtcgccgac aatgttgtac caccattgat gcgagacctc
     2341 atgcgcgatg atgcgaaaca atcgattgtg agtcgtgtcg tcgtagccag cgtagtcggt
     2401 cgaaccgatt gccacgaggc cgctgaattc catgctccac ggcccgctga cttcagcggc
     2461 gcgcaaggtc gaatagggat atgtgccata caactggccg tatagctgtg cggcgcgtac
     2521 cagcgtatac aatgcgggcc acgccaaaat tgtgtgtcga tcgaatgtat agaccgagta
     2581 ggtgacaccg ctgaaagtgt agtgggtttc aacatagtgc gaactggcca gcatgccaaa
     2641 agttctggcg cgatcgatcg cgtaatgcca cacattatcc tggtgcgaca tcaatccgcc
     2701 ggcaacgacc gtgacgcccg gcgtggccgt gatcgtcgcg gtgtagtcgg cgatatcgct
     2761 ggcgaacgga tcgccgatgg gataccacgg cacgctgatc cattttcctg atcgaaacgg
     2821 cgcgaggatc ggataccagt agccgatgtt ggtcgcttcg tcagtgtacg ccaagttcga
     2881 tgaagcgaaa ctttgcgtat cggccagcgc cggaatgtag acggtgaaat cgatcgtgag
     2941 atcaaccgaa gcgttgggtg cgagatcgga tggcagactg atggttaaaa cactgccgat
     3001 aagtttaacc cttccgggtg cttgattaag ttgtatgctg cgcagatcga atcgcgctcg
     3061 atcgtgggcg ggtggaacgt taacaacgat catcgagatc gatcgagtgt atgtattggt
     3121 gaagtgaatt tgttcaatcg cgtcgaggcg atgctgatcg t
//
LOCUS       DASRTJ010000398         6144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_24525,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000398 DASRTJ010000000
VERSION     DASRTJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24525"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFF70_08015"
     CDS             <1..517
                     /locus_tag="VFF70_08015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZY44678.1"
                     /translation="PVTVPVEMIAAFFPEGAPLQAPPRHPFPAAPPPPPDVYTIRRRV
                     SFSDIDMAQHVNNAVYLSYAEDAATAVLPQYGWPIERMREHGLAIIVRRHRIEYLQPA
                     LLGDEVEISTWVSNMRRASGTRHFMIGRAADHQLLARINTLALSINPITGLPTRPPDQ
                     FLSDFAPNIVA"
     gene            580..1128
                     /locus_tag="VFF70_08020"
     CDS             580..1128
                     /locus_tag="VFF70_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZY44679.1"
                     /translation="MKIEGEAQAGSQSDQPQSMALPVNAANMRKLRGLFAGGVSVVTT
                     QQADRLKGITVSAFVEVSLDPPLVLICVANEAESRALLEASKVFAVNILSDDQEFLSE
                     RFAARAPLVNDRFEGVPYHIASTGAPILDQAIAWYDCRVESIYAGGDHTLFIGRVVAI
                     GFDAEARRPLVYYANQYTRLKE"
     gene            complement(1311..1487)
                     /locus_tag="VFF70_08025"
     CDS             complement(1311..1487)
                     /locus_tag="VFF70_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44680.1"
                     /translation="MIQKWTTGQIIRVDILTPIGREFADGHNFEGTPTFVLFNRSGQE
                     INRWRRPPSINELP"
     gene            complement(1524..1799)
                     /locus_tag="VFF70_08030"
     CDS             complement(1524..1799)
                     /locus_tag="VFF70_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44681.1"
                     /translation="MTNDPLSDLLHFVNEHSYILAAAFLLLLLAIFLIARHVPNSVLI
                     WIGAAMVVTIIWFAARTGSGTQYASVDQYEAAIASGQPVLVEFYSDY"
     gene            complement(1796..2209)
                     /locus_tag="VFF70_08035"
     CDS             complement(1796..2209)
                     /locus_tag="VFF70_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44682.1"
                     /translation="MSNKALFPGVVVDEHGRALGVSMIGDEAQYVIDDNGFRRHIDAQ
                     TIDRQVLQTLQEQIEANKDIVEQQMLKMIGKDDLFTKAAVDQSLGNMDQVLERGLPDG
                     ALQWLGMMGFKIVVNLHGEVIKLDSPGVVDDSGDE"
     gene            complement(2296..3453)
                     /locus_tag="VFF70_08040"
     CDS             complement(2296..3453)
                     /locus_tag="VFF70_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZY44683.1"
                     /translation="MVVQRNPRAAAPKIDHRYDLFAACAPGFESVATAELFSLGIANP
                     KPVPGGVEFRGYLAHVYKLNLWSRTAERILLRVGSFHAITFKELRDNAKQIEWEKFLR
                     PGDRLALRVTCHKSRLIHSDAVAERVQLGITDRLGKASKILAKEEPLTQMISVRILDD
                     EATISLDTSGELLHFRGYRQAIAKAPLRETLAASMILASGWKSDQPLIDPFCGSGTIP
                     IEAALIACNIAPGLHRSFAFERWPKFDRSAWNKLIAQANESILLQAPSIIRGSDRDAG
                     AIEAGRLNAERAGVLAQIEFKQQAISDIQSIDHACIISNLPYGQRLAADKDLRNLYAQ
                     FGKILREKFSSGHVAILAGDVQLEKNTGLNFGDAVRINNGGINVRFVQVEI"
     gene            complement(3447..4442)
                     /gene="dusB"
                     /locus_tag="VFF70_08045"
     CDS             complement(3447..4442)
                     /gene="dusB"
                     /locus_tag="VFF70_08045"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZY44684.1"
                     /translation="MNAPRQPNFTIRDIPIYGDVILSPMAGFSDLPFRSICRAYGSAM
                     SYTEFVSAEAMSHNNPKTWRFFDFKPEERPVTFQFFDNDVERLVTQIKRAVDRCQPDI
                     VDINMGCSVSSVSQRGAGAGLLRTPAKIAQIFDRLTHELTIPITGKIRLGWDDDALNY
                     LEVAHVLEDNGASAIAVHGRTKAQAYKGEARWTPIGEVKAAVKIPVIGNGDVKRAADI
                     DRLKQQTGCDAVMIGRAAIGNPWIFQRKDRSDVSFTEKAQLIRRHLAAMMDYYGAASG
                     LIFFRKHVVKYIKGAQGVSDLHLPLVTCTTADQFIELFGQLEEKNRPQVELEGSW"
     gene            4557..5576
                     /locus_tag="VFF70_08050"
     CDS             4557..5576
                     /locus_tag="VFF70_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZY44685.1"
                     /translation="MPVRSDPARILILSNEDSTWTPRDYAEVDLELARMHDGLIESGY
                     QVEVFTVLHSVKEELGRQSRYHPADWLIVNWCEQYIDRPWTDAEIAQEIEDLGYVFTG
                     ADGRALRLSLDKTLVRRCLIEAGVTVPIGQVYHDDHLDDWKIFPAIVKPSNTHASDGI
                     TPASLVESPIELKRQVQAVLDQYQRPVIVEEYIDGREIHLPLLGNRSIEPIAPMEIDF
                     TLFNDIHDRFYTNEAKFDRESARYQLVRFLCPAPIDARLYARMVEIAQTAYRAVGCRD
                     YARIDLRLRGDQLYVLDVNHNADLTSEAEHSIASKMMGWSYGQLASRIIECALERWPD
                     RANSR"
     gene            5592..>6144
                     /locus_tag="VFF70_08055"
     CDS             5592..>6144
                     /locus_tag="VFF70_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44686.1"
                     /translation="MSLNTGPTQILMLHNHDNTWTEADLIEVAEENQKLMAGLRANGY
                     TVEDLKVTNSVAEVVRARNVDPREWLIFNWCEGYADRPWDYDGVTEELEQLQFVYTGS
                     GPWGLRVSRDKWLVRRLLAEAGTPLPLGGLMRTGTDVNWNLYPAIVKPVNQHASYGIS
                     REAIVENEHELKRRVEYVHETFQS"
BASE COUNT         1345 a         1618 c         1776 g         1405 t
ORIGIN      
        1 cccggtaacg gttccggttg agatgatagc cgcatttttc ccagaaggcg cgccgcttca
       61 agccccaccc cgccatccgt ttcccgctgc gccaccgcct ccaccggatg tctacacgat
      121 ccggcggcgc gtatcgttta gtgatatcga tatggcgcag cacgtcaaca acgctgtgta
      181 cctttcgtat gcagaagatg cggctaccgc agtgttgcca cagtatggct ggccgattga
      241 gcgcatgcgc gaacacggtc ttgccatcat tgtgcggcgg catcgcatcg aatatttaca
      301 gcctgcattg ctcggcgacg aagtcgaaat ctccacgtgg gtctcgaaca tgagacgggc
      361 ttccggcaca cgccacttca tgatcggccg ggccgctgat catcagttgc tggcgaggat
      421 caatactttg gcgttgtcga tcaatccgat tacaggtttg cccacgcgcc cgcccgatca
      481 atttctgtcg gatttcgccc cgaacattgt ggcatgatca gttgcggatt tgccctgaac
      541 gagtacaatc acatcaacca tttgccgatt ggataagaga tgaagatcga aggcgaggcc
      601 caagccggta gtcagtcaga tcagcctcag tcgatggcgc tgcccgtcaa tgcagcgaac
      661 atgcgcaagc tgcgcgggtt gttcgccggc ggcgtatcgg tggtgaccac gcagcaagcc
      721 gatcggttaa agggcatcac cgtcagcgcg tttgtcgaag tgtcgctcga tccgccgctg
      781 gtgttgatct gcgttgccaa cgaggccgaa tcgcgcgcgc tgctggaggc gagtaaagtt
      841 tttgcagtga atattctcag cgacgatcag gaattcttat cggaacgttt tgccgcgcgc
      901 gcgccgttgg tcaatgatcg attcgagggc gtgccgtatc acatcgcttc gactggcgcg
      961 ccgatcttgg atcaagcgat cgcctggtat gactgccgcg ttgaatcgat ctatgctggc
     1021 ggcgatcaca ccctctttat cgggcgcgtg gtagcgatcg ggtttgacgc tgaagcgcgt
     1081 cggccattgg tgtattacgc gaatcaatat acgcggttga aggaatgaga gagggcttgt
     1141 tattctgagt cgggcgaagc acgacgaaga atctctacca cgtcagcgtt cgatcttggt
     1201 aaccgcagag atgcttcgct cacctgcaat gctccgtggt acatcctgcg gctgcaccat
     1261 cgcaggacat tgcggcgcaa atgtcgctca gcatgacagg catcgtgtca ttacggcaat
     1321 tcattgatcg atggcggcct gcgccagcga ttaatttctt ggcctgatcg attaaacaat
     1381 acgaacgtcg gtgtgccttc gaagttgtgc ccatcggcaa actcgcgccc gatcggtgtg
     1441 aggatgtcca cgcgaatgat ctgccccgtt gtccacttct gtatcaaccc atcaacgata
     1501 ggcttggccg aaaggcaagc cagtcaataa tcggaataaa attcgacgag tacgggttga
     1561 ccggaagcga tagcggcttc atattgatcg actgacgcgt actgcgtgcc ggatcctgtg
     1621 cgcgcggcga accaaatgat cgtgaccacc atcgccgcgc cgatccagat gagtacacta
     1681 ttcgggacgt gtcgggcgat caggaagatc gccaagagga gcagcagaaa ggctgcggcc
     1741 aggatatagg aatgttcgtt gacgaaatgg agcaaatcac tcaaaggatc attggtcatt
     1801 cgtcgccgga atcatcgacg acgcccggcg agtcaagttt gatgacttcg ccgtgcagat
     1861 tgacgacgat cttaaagccc atcatgccca gccactgcag cgcgccatcg ggtaggccgc
     1921 gctccaaaac ttgatccatg ttgcccaacg attgatcgac ggccgctttg gtgaagagat
     1981 cgtctttgcc gatcatcttg agcatctgct gctcgacgat gtctttgttg gcctcgatct
     2041 gttcttgaag cgtctggagc acctgtcgat cgattgtctg agcatcgatg tgacgtcgaa
     2101 agccgttgtc gtcaatgacg tattgggctt cgtcgccgat catcgagaca ccgagtgcgc
     2161 ggccgtgttc atcgacgact acaccgggga ataaggcttt attgctcata atgattactg
     2221 ctcatcgcgc cgtcctcggc gcggaagatt tattttgaac gccgccgggg acggcggctt
     2281 acgcacgcac agaaattata tctctacttg cacaaaccgc acgttgatgc caccgttatt
     2341 gatgcggacg gcatcaccaa agtttaaccc cgtgttcttt tcaagctgca catcgccagc
     2401 cagaatggcg acgtgaccgc tggagaattt ttcacgcagg attttgccga attgggcata
     2461 caggttgcgc aagtccttgt cggcagccag tctttgtcca tacggtaagt tgctgatgat
     2521 gcaggcatga tcgatcgatt gaatgtctga tatggcctgt tgcttgaact cgatctgcgc
     2581 caacacgccc gcgcgttcgg cgtttaatcg gcctgcttcg attgcacctg catcccgatc
     2641 ggagccgcga atgatcgatg gcgcctgcag caagatcgat tcattggctt gcgcgattaa
     2701 cttattccac gcgcttcgat cgaacttggg ccagcgctcg aaagcgaatg atcgatgcaa
     2761 acccggcgca atgttgcacg cgatcaatgc ggcctcaatc ggaatggtgc ccgatccgca
     2821 gaacggatcg atcagcggtt gatcggattt ccaaccggag gcgaggatca ttgaagccgc
     2881 taatgtttcg cgcagcggcg ctttagcgat cgcttgtcgg tagccgcgaa aatgaagtaa
     2941 ctcgccggag gtatcgaggc tgatcgtggc ttcgtcatca aggatacgca cgctgatcat
     3001 ttgcgttaat ggttcttctt tggccagaat ctttgaggct ttgcccagtc gatcggttat
     3061 accgagctgc acccgttcgg cgaccgcatc tgaatgaatc aagcgggact tgtgacacgt
     3121 cacgcggagc gctaatcgat cgcccggcct taagaatttt tcccattcga tctgtttggc
     3181 gttatcccgt agttctttaa aagtgatcgc gtgaaatgat cccacgcgca gcaaaattct
     3241 ttcggcggtg cgtgaccaca agttgagttt gtagacgtgg gctagatagc cgcggaactc
     3301 gacgccgccg ggtaccggtt tggggttagc gatgccgagc gagaataatt cagcggtggc
     3361 aacggattca aaaccgggcg cgcaggctgc gaagagatcg taacgatgat cgatcttggg
     3421 cgcagccgcc cgcggattgc gttggactac catgatcctt cgagttcaac ctgcgggcgg
     3481 ttcttctctt ccaattggcc gaacagttcg atgaattgat cggcggtggt gcaggtcacc
     3541 agcggcagat gcagatcaga cacgccctgc gcgcccttga tgtatttcac gacgtgcttg
     3601 cgaaagaaaa ttaaaccgga tgccgcgccg tagtaatcca tcatcgccgc gagatgacgg
     3661 cggatgagct gggctttttc agtgaatgaa acgtccgatc gatctttgcg ctggaagatc
     3721 cacggattgc cgatggcggc gcggccgatc atcactgcgt cgcagccggt ttgttgctta
     3781 agccgatcga tatcggcggc ccgtttgaca tcgccattac cgataaccgg aatcttgacg
     3841 gcggctttca cttcgccgat cggcgtccag cgcgcttcgc ctttataggc ctgcgctttg
     3901 gtgcggccat gaacggcgat cgcggacgcg ccattgtctt ccagaacgtg cgccacttcc
     3961 aaataattca gcgcgtcatc atcccagccc aatcgaatct tgccggtgat cggaatcgtt
     4021 agttcgtgcg tcaaccgatc gaatatctgt gcgatcttag cgggtgtgcg caataagccc
     4081 gcgcccgccc cgcgctgaga gacactggac accgagcaac ccatgttgat atcgacgata
     4141 tcgggctgac agcgatcgac tgcgcgtttg atctgtgtca ccaagcgctc gacatcatta
     4201 tcaaagaact ggaacgtcac gggacgctct tccggcttga agtcaaagaa gcgccaggtt
     4261 tttggattgt tgtggctcat cgcttctgct gagacgaatt cagtgtagct catcgccgaa
     4321 ccataggcgc ggcagatcga ccgaaacggc agatcggaga agccggccat gggcgagagg
     4381 atcacatcgc cgtagatcgg aatatcgcga atagtgaagt tgggttggcg tggtgcattc
     4441 atcgcaaatg attataccag aggtgaagtg tgatacgtga tgcgtgagag acggttcacg
     4501 tatcacgcat cataattcat gttacaataa aattgaataa tcttatgagg tggtgcttgc
     4561 ccgttcgatc agatccggct cgtatcttga ttttgagtaa tgaagacagc acttggacgc
     4621 cgcgcgatta tgctgaagtc gatctggaac ttgcgcgcat gcatgatggc ttgatcgagt
     4681 ccggctatca ggtagaagtg ttcacggtgc tgcacagcgt taaagaagaa ttgggtcgcc
     4741 agtcgcgcta tcatccggct gactggttga tcgtcaactg gtgtgaacag tacatcgatc
     4801 gaccgtggac ggatgcggag atcgcgcagg agatcgaaga cctcggttat gtgtttaccg
     4861 gcgctgatgg ccgcgcgctg cgcttgtcgc tggataaaac gctcgtgcgc cggtgcttga
     4921 tcgaagccgg cgttacagtg ccgatcggcc aagtctatca cgacgatcat ttggacgact
     4981 ggaaaatttt tccggcaatc gtcaagccca gcaacacgca tgccagcgac ggcatcacgc
     5041 ccgcctcgtt ggtcgaatca ccgatcgagt tgaagcggca agttcaagcg gtgctggatc
     5101 aatatcagcg gccggtgatc gtcgaagaat atatcgatgg ccgcgagatt catctgccgc
     5161 tgttgggcaa ccgatcgatc gaaccgatcg cgccgatgga aattgatttc accttgttca
     5221 acgacataca cgatcggttc tacaccaatg aagcaaagtt cgatcgagag tcggcccgct
     5281 atcaacttgt tcgattcttg tgcccggcgc cgatcgatgc gcgcttgtac gcgcgaatgg
     5341 tcgagatcgc gcagacagct tatcgggcgg tgggctgccg agactatgcc cgcatcgatc
     5401 tgcggctgcg cggcgatcag ctctacgtgt tggatgtcaa tcacaatgct gatttaactt
     5461 ctgaggcgga acattcgatc gcttcgaaga tgatgggctg gagctacggg cagctggcgt
     5521 cacgcattat tgagtgtgcg ttggaacgct ggccagaccg cgccaacagc cggtaatggg
     5581 aaagatttag catgtcattg aatactggac ctactcaaat cttgatgctg cacaatcacg
     5641 acaatacatg gactgaagcc gatctcattg aagtggctga agaaaatcaa aagttaatgg
     5701 cgggcttgcg cgcgaacggc tataccgtcg aagatttgaa agtcaccaat agcgtggccg
     5761 aggtggtgcg cgcgcgcaat gtcgatccgc gcgagtggtt gatcttcaat tggtgcgaag
     5821 gctacgccga taggccgtgg gactatgatg gcgtgacgga ggagttggag cagctgcaat
     5881 tcgtgtacac cggctccgga ccgtgggggc tgcgcgtctc gcgcgacaag tggctggtgc
     5941 ggcggctgtt ggccgaagcg ggtacaccct tgccgctggg cgggttgatg cgaaccggaa
     6001 cggatgtgaa ctggaatctt tatccggcca tcgtcaagcc ggtcaatcaa catgccagtt
     6061 atggcatcag ccgcgaagcg atcgttgaaa atgagcacga gctgaaacgg cgcgtggaat
     6121 atgtgcacga gacatttcag tcgg
//
LOCUS       DASRTJ010000399         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_62740,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000399 DASRTJ010000000
VERSION     DASRTJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62740"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFF70_08060"
     CDS             <1..1197
                     /locus_tag="VFF70_08060"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY44687.1"
                     /translation="TAILNTIISGNTANSACGLDCAGGGGVFNQGGYVSLSDMSVIGN
                     ASLGGIGNVGGGLFNLNGGVMEIKNVDLNRNTSNESGGGAFNGYGSYMEMSVDRVMTN
                     TVAQYGGGVVNSGTLVIDNSIFAGNVVTADAIFVGGGAIQNFGILTVTASTIASNTAR
                     LGGGIQNDAVAVLALINSSISYNSATNYAGLSLSGQVSVLNSTIDHNTSLGNGAGINN
                     RGNLALTNSTISSNRISFGGWGAGLDNQSGMAQLINVSVISNTSTIGGAGFHTLGALY
                     LTNTLLAGNVWMSSAQANCGGTTYTSRGYNLSSDATCTLLNATGDMTNVNPHIGPLAN
                     NGGSTWTHALRVGSPAIDRGTNAGCPSTDQRGIARPQLVKCDIGAYEYDGVLKIVFLP
                     LTLKNY"
     gene            1273..2283
                     /locus_tag="VFF70_08065"
     CDS             1273..2283
                     /locus_tag="VFF70_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZY44688.1"
                     /translation="MKGISHFLAGVAAATFVPGVVANAVDGSFLIALGGFFGILPDTI
                     DFKFTRYFDKAVGIDPDPANLDPQWIADQLAALIDRAGETNQAVRVQLHSIKLGPDRW
                     RQYTIRFDSDRNEVAVRIGPIVNTSQMPYPDTEIAGLKTARAALKAKMVYTYDGDMNV
                     DIFSGPSFAFSPKPHGVEVQFLPWHREWSHSLTLAAFLGLLVAVIGQSVQAGMVAGFA
                     MTAHILEDQLGYLGSNLFWPFTKDRSPGLKLIHSGDAIPNFLAVWTALCLILFNLDRF
                     SAPIQHRSNMLNPVSFFLLAIAAPYVILLSQYVIGKRKQHMRLEASRQADLVAELQET
                     EV"
     gene            2326..>4273
                     /locus_tag="VFF70_08070"
     CDS             2326..>4273
                     /locus_tag="VFF70_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HZY44689.1"
                     /translation="MKLTRALLVTFIGFVAVSGLLLILIPAQAAPLRTTAQLALEVVI
                     NEVAWGGTAANANHEWIELKNNTTATVNLAGWHLYGTGGATPPNIILSGTIPISGYFL
                     LERATDTAVSDISADQIYPVGTLLNSGAVLTLTDSLSNVIDTANGNGGAWPGGSGSPN
                     YYSMERIDPLSPDTDSNWASNDGITRNGLDANSNPITGTPKQQNSVYAPYVPPPPLTP
                     PGSVLISAVHFSAYANGDEGFRLTNVTTQVITLTNWIAHDNSQSLPLTGTLDPSQSIW
                     LAKRAITFTQQFGSKPDYEYELDSDPSVPNLPVIGSIPALSADDALAIREGASNWIDG
                     VVWGNGHISDTGWFTGWIGSNLYPYTGTLSASGDIIYRKLDETSGSIITDTGTALDWA
                     NDRTDPISGRKVMYPGWDLEKFWQTAKVTATSHLTVAIAPDNAFRVISDFLGSAQHSI
                     KIEIYSFDNVGLVAAITRTLQRGVSVTILMEGSPVGGLPDEEKWVCQQIEGAGGQCWF
                     MIGDSAQKIHDRYDYIHAKMTIIDDQVVAIGSENFSPRSLPNDDFSDGTLGQRGTYLI
                     TDAPGVVTRALEIWNTDFDPIHHRDIYRWTSGDPNYGAPPIQFTPVYSTGGTGYRIYF
                     PTPRHRSNCWRLHGSPTRHRPIKRE"
BASE COUNT          937 a         1154 c         1200 g          982 t
ORIGIN      
        1 accgctatct tgaatacgat catcagcggc aatacggcga atagcgcatg cggcctcgat
       61 tgtgcgggcg gcggcggagt ctttaatcaa ggcggttacg tttcactgtc ggacatgtcg
      121 gtgatcggta atgccagctt aggcggtatt ggtaacgtcg gcggcggcct cttcaatctt
      181 aatggtggcg tgatggagat caagaacgtc gatctgaatc gcaacaccag caacgaaagc
      241 ggcggcggcg ctttcaatgg ttatggcagc tatatggaaa tgagtgtcga tcgggtcatg
      301 acgaatacgg tggcgcaata cggcggcggg gtggtcaaca gcggcacgct cgtaatcgat
      361 aacagcatct tcgcaggcaa tgtcgtgacg gctgacgcta tctttgtcgg cggcggggcg
      421 attcaaaatt ttggaatact gacggtgaca gccagcacga tcgccagcaa cacggcgcgt
      481 ttgggcggcg ggattcaaaa tgatgcggtt gcggtgctgg ccttgatcaa ttcgtcgatc
      541 agttataact ccgcgactaa ctatgcgggt ctctcgctca gtggtcaagt ttcagtgctg
      601 aattccacca tcgatcacaa tacgagcctt ggcaacggcg ccggcatcaa taatcgtggc
      661 aatctcgcgt tgaccaattc gactatcagt agcaatcgca tttcgttcgg cggttggggt
      721 gcgggcctcg ataatcagag tggtatggct cagttgatca atgtcagtgt gatcagcaac
      781 acatcgacaa ttggcggggc tggctttcat accttgggag cactgtatct gaccaacacc
      841 ctgctggctg gaaatgtgtg gatgtcaagc gctcaggcga attgcggcgg aacgacttac
      901 acttcgcgcg gttataattt gagcagcgat gcgacttgca cgttgttgaa cgcgacgggc
      961 gacatgacga atgtgaatcc acatatcggc ccattagcca acaatggcgg atcaacctgg
     1021 acacatgcgc tgcgggtggg cagtccggcg atcgatcgtg gcacaaatgc aggttgcccg
     1081 tcaaccgatc agcgcggcat cgcccgaccg caattggtca agtgtgacat cggcgcgtat
     1141 gaatatgacg gcgtgttgaa aatagtgttc ctgccgttga cgttgaaaaa ttattaacgg
     1201 taggggcgag gtcaccacgc cctatcatta agcgggcgag gcgacctcgc ccgctacagg
     1261 acggtgaatg aaatgaaagg catctcacat tttctggcag gcgtagccgc cgcgaccttt
     1321 gtgccgggcg tcgtcgccaa cgcggtggac ggatcattct tgatcgcgct gggcggcttc
     1381 ttcggcattc tgcccgacac gatcgatttc aaattcacgc gctacttcga taaagccgtc
     1441 ggcatcgatc ccgatccggc caatcttgat ccgcagtgga tcgcggatca gctggccgcg
     1501 ctgatcgatc gggccgggga aaccaatcag gcggtgcgcg ttcaactcca ttcgatcaaa
     1561 ctggggcccg atcgctggcg gcagtacacc atccgcttcg actccgatcg aaatgaagtg
     1621 gcggtgcgca tcggcccgat cgtcaacacc tcgcagatgc cgtatcccga tactgagatc
     1681 gccggtctca agaccgcgcg cgccgcgttg aaagcaaaga tggtctacac ctacgatggc
     1741 gacatgaacg tcgatatttt cagcggcccg tcgtttgcgt tttcgccgaa gccgcacggc
     1801 gtggaagtgc agttcctgcc gtggcatcgc gagtggagtc attcgttgac gctcgccgcg
     1861 tttctgggct tgctcgtcgc ggtgatcgga caatctgtgc aggccgggat ggtcgctggc
     1921 ttcgccatga ctgcgcatat tcttgaagat caactcggtt atctgggcag caatttgttt
     1981 tggcccttta ccaaagatcg atcgccgggg ctgaagctca ttcactccgg tgacgccatc
     2041 ccgaattttc tggcggtgtg gacagccttg tgcctgatcc tcttcaatct cgatcggttt
     2101 tctgcgccca ttcagcatcg atcgaacatg ctcaatcccg tttcgttttt cttgttggcc
     2161 atcgctgcgc cctatgtgat cttgctcagc caatacgtga tcggtaaacg caagcagcac
     2221 atgcgccttg aagcgtcgcg gcaggctgac ctggttgccg aattgcagga gacggaagtt
     2281 tagcctgcgt gtagatttga tcacatcggg cgaatgaggg ataacatgaa gctcactcgt
     2341 gcgctgttag tgacattcat cggtttcgtt gctgtctcag gcttgttgct gatcttgatt
     2401 ccggcacagg ccgcgccgct gcgcaccacg gctcagttgg ccttagaggt tgttattaac
     2461 gaagtggctt ggggaggaac ggcggcgaat gccaatcacg agtggatcga attaaagaat
     2521 aatacaacgg cgacagtcaa tttggctggc tggcacttgt atggcactgg cggcgcgaca
     2581 ccgcccaata ttattctctc tggcacaatt ccgatcagcg gttactttct attagagcgt
     2641 gccaccgata cagctgtgag cgacatttcg gccgatcaaa tctatcctgt aggtacactc
     2701 ctaaattccg gtgcagttct aacgctaacg gattcactta gcaatgtgat cgacacggcc
     2761 aatggcaatg gcggtgcctg gcccggcgga tcgggttcgc ccaactatta ttcgatggag
     2821 cggattgatc cattatcacc cgatacagac tcgaactggg ccagcaatga cggcatcacg
     2881 cgtaatggct tagatgcaaa cagtaatcca atcaccggca cacccaaaca acagaactct
     2941 gtctatgcac cttatgtgcc accacctcca ctcactccgc ccggcagcgt cttgatcagc
     3001 gcggtgcact tcagcgctta cgccaacggc gacgaaggct ttcgtttgac gaatgtcacc
     3061 acgcaagtga tcacgctgac gaattggatc gcgcatgaca acagccagtc actgccgctg
     3121 actggtacgc tcgatccgag ccagtcgatc tggttagcta agcgcgcaat tacttttact
     3181 cagcaatttg gttccaagcc cgattatgaa tacgaactcg attccgatcc cagtgtgcca
     3241 aatttgccag tgatcggcag tattcccgct ttatcggccg atgacgcgtt ggcgatccgt
     3301 gaaggcgcca gcaattggat cgatggcgtg gtgtggggca atgggcacat ctcggatacg
     3361 ggttggttca ccggctggat cggatcgaat ctgtatcctt ataccggcac gctatctgca
     3421 tcgggtgaca tcatctatcg caaactggat gaaaccagcg gctcgatcat aacggacacc
     3481 ggtaccgcgc tcgattgggc gaacgatcga accgatccga tttcgggccg caaggtcatg
     3541 tatcccggct gggatttgga aaaattctgg cagaccgcca aagtcacagc tacttcacac
     3601 ctgactgtgg ccattgcacc cgataatgct ttccgcgtta tctcggattt tctcggctcg
     3661 gcgcagcact cgatcaagat cgaaatttac tcgttcgata atgtcggctt ggtggccgcg
     3721 attacgcgca cactacagcg cggtgtcagc gtgacgatct taatggaagg cagcccggtc
     3781 ggcggtttgc ccgatgaaga gaaatgggtg tgtcagcaga tcgagggtgc gggcggccaa
     3841 tgctggttca tgatcggcga cagcgcgcag aaaattcacg atcgctacga ttacattcac
     3901 gccaaaatga cgatcattga tgatcaggtt gtggcaatcg gttcagagaa cttcagtccg
     3961 cgcagcctgc cgaacgatga cttcagcgac ggaacactcg gccagcgcgg cacatacctc
     4021 atcactgatg cgcccggtgt ggtcacgcgc gcgcttgaaa tctggaatac cgattttgat
     4081 ccaatccatc atcgggatat ttatcgctgg accagtggcg atccgaacta tggtgctccg
     4141 ccgattcagt tcacgccggt ctattcaacg ggcggcactg gctatcgaat ttatttcccc
     4201 acaccacgcc atcgatccaa ttgctggcgc cttcacggat cgccaacgcg tcatcggccg
     4261 ataaagcggg aat
//
LOCUS       DASRTJ010000400         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_24644,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000400 DASRTJ010000000
VERSION     DASRTJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24644"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..609
                     /locus_tag="VFF70_08075"
     CDS             103..609
                     /locus_tag="VFF70_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HZY44690.1"
                     /translation="MIIVSDTSPILSLAIIDRLELLEQLYGKISIPPAVRDEIAVQGD
                     NQPGAHQVTAMKWITTISTGNPIVVMLLLRELDLGEAEAIALALELKADLLLLDDRKA
                     RSLAAYLGIPFAGLIDILNAAKHAKLIPGIKPVLDELMAQAKFRVSRKLYQRVLQDAG
                     EAKSAVAA"
     gene            593..1387
                     /locus_tag="VFF70_08080"
     CDS             593..1387
                     /locus_tag="VFF70_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZY44691.1"
                     /translation="MPSLRELTEYHKPTNLDEAIKLLQRPQVRTVPLAGGTQLVPSAA
                     HHIEAVVDLSSLNLSYIKSTSQGIAIGATTTLQQIVDDEIMGEYADGVLITAMLDSAS
                     LNTRNAASIAGSIVGAAGNSPLLTTLLALDARLIVRGDREQEVSVANWVAQHGTLILK
                     INLPALADHTHLAYEKVARTPADLPIVCVAVRATIDKNRLANVRLALGGVAGKPIVIE
                     KAESTIEQAAQLAVESIDPPSDYFATAEYRREMIDVLVKRTLKSLV"
     gene            1413..>2085
                     /locus_tag="VFF70_08085"
     CDS             1413..>2085
                     /locus_tag="VFF70_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HZY44692.1"
                     /translation="MPDPLTSVQSQIIRTPDQRLRVFVSSTLQELADERIAAREAIEH
                     LRLTPVMFELGARPHPPKDLYRAYLDQSHIFIGIYWQKYGWVAPDMTISGLEDECNLS
                     GQRPKLIYLKSPAPDRESRLKDLLDRIRNDDRVSYKSFATAAELRELIENDLALLLTE
                     HFEQARSVLAPSTAPASPPRHNLPTPPTRLIGREKELVEIQNHLLKEDVRLVTLTGPG
                     GTGKTR"
BASE COUNT          500 a          580 c          546 g          459 t
ORIGIN      
        1 gatcaccgcc gaagcggatc tcctcgtggc gcggccaccg caggattttc gcgatcgatg
       61 ggaacacgaa aaacagttct ggaaagtgta tcccgatcca gtatgatcat cgtcagcgat
      121 acttcaccga tactcagcct cgccattatc gatcggcttg agcttcttga acagctctat
      181 ggcaaaattt cgatcccgcc ggcggtgcgc gacgagatcg ccgttcaggg cgacaatcaa
      241 ccgggcgcgc atcaagttac tgcgatgaaa tggatcacaa caatatcgac cggtaacccg
      301 attgtggtga tgctcctctt aagagaattg gatctgggtg aggcagaagc tattgcgttg
      361 gcgctggaat tgaaagccga tctgctgcta ttggatgatc gcaaagcgcg atcgctcgcc
      421 gcatatttgg gcataccgtt cgcgggcttg atcgatattc tcaacgcagc caaacacgcg
      481 aagttgattc cggggatcaa gccggtgctc gatgagctga tggcgcaagc caaattccgg
      541 gtcagccgca aactttatca gcgcgtcctg caagacgcgg gagaagccaa aagtgccgtc
      601 gctgcgtgaa ctcactgaat atcataaacc aaccaatctc gacgaagcga tcaagttgtt
      661 acagcgtccg caggttcgaa ccgtcccgct ggcaggtgga acgcagctgg tgccatccgc
      721 ggctcatcac attgaagcgg tcgtcgatct gagcagcttg aatctatcgt atataaaatc
      781 gaccagccaa ggcatcgcca tcggcgcaac gacaaccttg cagcagattg ttgacgatga
      841 gatcatgggt gagtacgccg atggcgtttt gatcacggcg atgttagatt cagcgtcatt
      901 gaatacgcgc aatgcggctt cgatcgccgg atcgatcgtc ggggccgctg gtaactcgcc
      961 gctgctcacc actttactcg cgcttgatgc gcgcttgatc gtacgcggcg atcgagagca
     1021 agaagtctca gtcgcgaatt gggtcgcgca acacggaact ttgatcttaa agatcaactt
     1081 acccgcttta gctgatcata cgcacctagc ctatgagaaa gtcgcacgca caccagccga
     1141 tctgccgatt gtgtgcgttg cagtgcgcgc aacgatcgat aagaaccggc tagcgaatgt
     1201 acgactcgca cttggcggtg tcgccgggaa accgatcgtg atcgagaaag ccgaaagcac
     1261 gatcgagcaa gctgcacagc tggccgttga atcgatcgat ccgccatccg attactttgc
     1321 gaccgccgaa tatcggcggg aaatgatcga tgtcttagtc aaacgtacac taaaatcttt
     1381 ggtctgatct gattcgtcca gttttcattg tgatgcccga cccattgacc tctgttcaat
     1441 ctcaaataat ccgcacacct gatcagcggc tgcgcgtttt tgtcagttca acgctgcagg
     1501 aactcgccga cgaacgcatc gctgcgcgcg aggcgattga gcatctgcgt ttaactcctg
     1561 tgatgttcga acttggcgcg cgaccccatc cacccaaaga tttgtatcgc gcgtatctcg
     1621 atcaatccca catcttcatc ggcatttatt ggcagaagta tggctgggtc gcgcccgaca
     1681 tgacgatcag cgggcttgaa gatgaatgca acttatccgg tcagcgaccg aaactgatct
     1741 atctcaaatc tccggctccg gatcgcgagt cccgattgaa agacctgctt gatcggattc
     1801 gcaacgacga tcgggtctca tataaatctt tcgccacagc cgccgaactg cgcgagttga
     1861 tcgagaatga cctcgcacta ttgctgaccg agcatttcga gcaagcccga tcggtccttg
     1921 ctccatctac ggcaccggca tctcccccac ggcataactt gccaacacca cccactcgct
     1981 tgattggccg cgaaaaagaa ttggtggaaa ttcaaaatca tttattgaaa gaggacgttc
     2041 gactggtcac gctgacggga ccgggcggca ctggcaaaac ccggc
//
LOCUS       DASRTJ010000401         5557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_162025,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000401 DASRTJ010000000
VERSION     DASRTJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162025"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFF70_08090"
     CDS             <1..508
                     /locus_tag="VFF70_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HZY44693.1"
                     /translation="AFLSYYAELGAPPGYVVPIHDQASAHDPANYRRLVQAGLIYIGS
                     GDTQRLIEAIEGTPAIEAIAEAFDNGAVIIGARAGAMLLGKWGLSLSKQKTYPGWGWI
                     SDAVITPRYTPDRTADIRSVLQRYPEAISIGIPAEVALALGPEGEVDTWTDTDEQVTV
                     TLGAKFGR"
     gene            575..2545
                     /locus_tag="VFF70_08095"
     CDS             575..2545
                     /locus_tag="VFF70_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZY44694.1"
                     /translation="MATSMSASPAPVKKKNRYTHLWNLIGITVVLGIIAVALGVQLKV
                     PGFLGTRATTGAGLAVILEIAGLIALWIGRGLARKKDFYHHQRVQTAVMLFNIVVILF
                     VMVVTFSQQVVGPGAPPNDPVVLIEIGHGISGVLLQLLGLYIVVRMWFEKVLPQWFLF
                     KNFRLLMLTTLFWWTLQTLGGVGIFAMRYLTAAPAAAPIVSTAPTAVAAVATAIPQPT
                     LAPVPTAVPTQPPVTGSAAARDDQVHSDSLLIRFQDVPQPASGEQYVGWLIGNGGEFR
                     LRLGTLKVDADGKVAAQYTSPKGSNLLGLYNEFLISTEAAGSTPTAPSDKLAFSVIVP
                     PKARASLNQLLVDAPDTPFNTGYLLGLLGQGRRVVNHAELAMHFADKNNLKDVQRQAE
                     LIVNLIEGELGANFGDVDGDGRVFNDGNGIGIFPRSIDKVKQVAQAAIDASDATESIK
                     LHMGHTIIGVDNVIPWAQQIDALALALAKTKDLAEAKKMVVDLNSLAQKLLQGVDLNN
                     DETIDPVKGEGTIEVAYKHAQFAASPVYNAPLVVGGAPLVVAEVTPTPAPTQPPAPTA
                     GPQAVSVALIDFAFDQPTLTIKVGTTVSWINRDPAPHTITFDDGSFDSGTLAQGQTAS
                     FTFDKAGEFAYYCLFHGGPGGVGMTAKVIVAP"
     gene            2664..2921
                     /locus_tag="VFF70_08100"
     CDS             2664..2921
                     /locus_tag="VFF70_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019251896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HZY44695.1"
                     /translation="MTDEKVFTEKELQSYNGMRGRSIYIAYGGIVYDVTHAPKWQTGF
                     HEDLHYSGIDLTRSLPKAPHGEEVFVRPGVKRVGRLITDRE"
     gene            3072..4118
                     /gene="coxB"
                     /locus_tag="VFF70_08105"
     CDS             3072..4118
                     /gene="coxB"
                     /locus_tag="VFF70_08105"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZY44696.1"
                     /translation="MIRLQRNSVRRALRIVAVLLSLFTIALLNALPVLAGPNTPPSTL
                     DPKGTNAATIAGLFNIVLIIAVIVFAVVEGLLLFSAWRFRHKPAGAVEPPQIHGNTRF
                     ELAWTIIPALIVVSLFVVTLQTQQKLDNKGSTEPITVKVIGHQWWWEYQYTDLGITTG
                     TDLVIPTGRTVNLEITSIDVIHSFWIPQLNGKTDAIPNHINTSWLEADQPGTYFGQCA
                     ELCGDSHANMRIVVIAKDDADFQAWVSAQQAGPAQPTTPDATAGQQVFMTGPCVGCHA
                     INGTAAVGKVGPNLTHFGSRTHIAGGMFENTSGNLTRWLGNPPGVKPGSIMPNLHLNP
                     ADVQKLVAYLESLQ"
     gene            4196..>5557
                     /locus_tag="VFF70_08110"
     CDS             4196..>5557
                     /locus_tag="VFF70_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZY44697.1"
                     /translation="MATAQAAMPRTLIVKSGLKAWLTTVDHKKIGILYILSSVFFFIV
                     GGVLALVMRLELAQPGLPVVSAEFYNQAFTMHGTTMIFLFIIPIFAGIGNYIVPLQVG
                     ARDMAYPRINALGFWMIVGGALILYSSFLVGAPNAGWTGYSPLANSIFSKSAGTDMWI
                     LGLLLIGTSSLLGAINFIVTILNMRAPGMTLTRMPLFCWSMLTTSLMILASTPVLTGA
                     FIMLYADRNFGTQFFSGAGSDPLMWQHMFWFYSHPAVYIMILPAMGIVSEILPVFSGK
                     PIFGYKAIAYSTAAIAILGFMVWAHHMFATGMSPILKVFFMTATMTIAVPTGVKIFNW
                     IATIWRGHLRFHTPLLFIFGFLSMFVIGGLSGMFSAVVPVDLQITDTYFIVAHLHYVL
                     FGGSVFGIFAGLYYWTPKMTGWLMNEKLGKLHFWLMFIGANLAFFPQHFLGLAGMPRR
                     YADY"
BASE COUNT         1161 a         1482 c         1583 g         1331 t
ORIGIN      
        1 agcgttcctg tcgtattacg cggagttagg cgcgccgccc ggctatgtgg tgccgattca
       61 cgatcaagcc tcggcgcatg atccggccaa ctatcggcgc ttagtgcaag ccggattgat
      121 ctacatcggc agcggcgata cgcagcgttt gattgaagcg atcgaaggca cacccgcgat
      181 cgaagcgatc gccgaagcgt tcgataacgg cgcggtgatc atcggcgcga gggcgggcgc
      241 tatgttgtta ggcaagtggg gcttatcgct ctcaaagcaa aaaacttatc cgggctgggg
      301 ctggatatcg gatgcagtga ttacgccgcg ttacacgccc gatcggacag ccgacatccg
      361 atcggtattg cagcgatatc ccgaagcgat ttcgatcggt attccggctg aagttgcatt
      421 ggcgctcggc ccggagggcg aggtcgatac ctggacggat acggatgagc aagtcaccgt
      481 gaccctgggt gcgaagtttg gacgataagc aataaacatc gattgcaaag ttgcgtgcgt
      541 ttccatacga tccaattatt aggggaggca gattatggct acatcaatga gtgcatcacc
      601 ggctcctgtt aagaaaaaaa atcgatatac acatctgtgg aatctgatcg gcattaccgt
      661 tgtgctgggc atcatcgccg tcgcgctcgg cgtgcaattg aaagtgccgg gctttctggg
      721 cacgcgtgcc acaacaggcg ctggccttgc cgtcatcttg gaaatcgccg gactgatcgc
      781 attgtggatc ggccgcgggc tggcgcgcaa aaaagatttc tatcatcacc agcgcgtgca
      841 gacggccgtg atgttgttca acatcgtcgt cattttgttt gtgatggtgg tgaccttctc
      901 gcagcaagtc gtcggtcccg gtgcgccacc gaacgatccg gtggtgctga ttgaaattgg
      961 acatggcatc agcggcgtcc tgctgcaatt gctcggtctg tacatcgttg tgcgcatgtg
     1021 gttcgaaaaa gttttgccgc agtggttctt gttcaagaac ttccgcttgt tgatgttgac
     1081 gacgttgttc tggtggacgc tgcaaacact cggcggcgtt ggaatttttg cgatgcgata
     1141 tttgacagcg gcgccggcgg cagccccgat tgtttccacg gccccgacgg ctgttgctgc
     1201 cgtggcaacc gctattccac aacctacact tgcgcctgtg ccaacagcag tgccgaccca
     1261 gccgcccgtg actggctcgg cggccgcgcg cgacgatcaa gttcacagcg attcactgct
     1321 aatcagattt caggatgttc cgcagccggc cagtggcgaa cagtatgtcg gttggctgat
     1381 cggcaatggc ggcgagttcc gattgaggct gggcacgttg aaggttgatg ccgacggaaa
     1441 agttgctgcg caatacactt cgccgaaggg ctcgaatctg ctggggctgt ataacgaatt
     1501 cttgatctcg actgaagcgg ccggctcaac gccgactgcg ccttccgata agctcgcttt
     1561 ctccgtgatt gtgccgccta aagcgcgcgc atctctgaac cagttgttgg tcgatgcacc
     1621 ggacacaccg ttcaacaccg gttatttgtt aggcctgctg ggccaggggc ggcgcgtagt
     1681 caatcacgcc gaactcgcca tgcacttcgc cgacaagaat aatttgaagg acgtccagcg
     1741 tcaagccgaa ttgatcgtca acttgatcga aggcgagctg ggcgcaaact tcggcgatgt
     1801 cgatggcgat ggccgagtat tcaatgacgg caatgggatc ggtatcttcc cccgatcgat
     1861 cgacaaagtc aaacaagtcg cgcaagccgc gatcgatgcg tccgatgcta ccgagtcgat
     1921 caaattgcac atgggccaca cgatcatcgg cgtggacaat gtcattccgt gggcgcagca
     1981 gattgatgca ctggctttgg cgctggcgaa gaccaaagat ttggctgagg ctaaaaagat
     2041 ggtcgtggat ctcaattcat tggcccaaaa attgttacag ggtgttgatc tgaataacga
     2101 cgaaacaatc gatccggtga agggtgaagg cacgatcgag gtagcatata agcacgcgca
     2161 gttcgcagcg agtccggttt ataatgcgcc cttagttgtc ggtggcgcgc cgttggttgt
     2221 ggctgaagtc acgccgacac cagccccgac ccagccgcct gcgccaactg ccggacctca
     2281 agctgtgagc gtggcgctga tcgattttgc gttcgatcaa cctacgctta ctataaaagt
     2341 cggcacaacg gtctcgtgga tcaatcgcga tccggcgccg cacaccatca ccttcgacga
     2401 cggctcattc gatagtggca cattggcgca aggacaaacc gcttcgttca cattcgataa
     2461 agccggcgag tttgcctact actgtttgtt tcacggcggg ccgggcggcg taggcatgac
     2521 tgcgaaagtg atcgttgcac cttaagccgc ctctattcgg ccacggaatt tgtttctcaa
     2581 gtcgccgtcc ccggcgtagt tgatgccgtt ggggacggcg ttaagcttga ttgggctgag
     2641 caatgatcga tccgcaacgt gatatgactg acgaaaaagt tttcaccgag aaagaactgc
     2701 agtcgtataa cggcatgcgc ggccgctcga tctacatcgc ctatgggggc attgtttatg
     2761 atgttactca cgcaccgaag tggcagactg gctttcacga agatttgcat tacagcggca
     2821 tcgatctaac acgcagtttg cccaaagcgc cgcacggcga agaagttttc gtgcggcccg
     2881 gcgtgaaacg cgtcgggcgg ctgatcaccg atcgggaata agaaagatcg gctgacttga
     2941 aagtcgataa gagcgtggta taatgccgag cgttaaaggt tgaaaccgcg ggcactgatc
     3001 gaataaattc agtgcccgtt gcttgcacgc tagacacaaa taaacttggt tagctggttt
     3061 tgggaggata aatgattcgg ttgcagcgaa attccgtccg ccgcgcgcta cgcattgtcg
     3121 cggtgctgct gagtctcttt accattgcgc tcctcaacgc tttaccggtt ctggccggtc
     3181 ccaacactcc accttctacg ctcgatccca aaggcaccaa cgccgcgacg attgccggcc
     3241 tgttcaacat tgtgttgatc atcgccgtga tcgtcttcgc cgttgtggaa gggctgctgt
     3301 tattctcggc ctggcgcttc cgtcacaagc cagcgggcgc cgttgaaccg ccacagattc
     3361 acggcaacac ccgctttgaa ttagcctgga cgattatacc ggcgttgatt gtcgtatcgc
     3421 tctttgtcgt gacgctgcag acgcagcaaa aacttgacaa caagggcagc accgagccga
     3481 ttactgtgaa ggtcatcggc catcaatggt ggtgggagta tcagtatacc gatctgggca
     3541 ttaccactgg cactgatctg gtgattccca ctggccgcac cgtcaacctc gaaattacct
     3601 ccatcgatgt gatccacagt ttctggattc cgcagctcaa cggcaagacc gatgcgatcc
     3661 cgaatcacat taacaccagt tggctggaag ccgatcagcc cggcacgtat tttggtcagt
     3721 gcgccgagtt gtgcggtgat tcgcatgcca acatgcgcat tgtagtcatt gccaaagacg
     3781 acgcagattt tcaagcatgg gtcagcgctc aacaagctgg cccggctcag ccaacgacac
     3841 cggatgcgac ggcgggacag caagtgttta tgaccggtcc gtgcgtaggc tgccatgcga
     3901 tcaatgggac agccgcagtc ggcaaagttg gaccgaattt gacgcatttc ggcagtcgca
     3961 cgcatatcgc aggtggcatg tttgaaaata catcgggcaa tcttacacgt tggctcggca
     4021 atccgcccgg tgtgaagccc ggatcgatca tgccgaattt gcatttgaat ccggctgatg
     4081 ttcaaaagtt agtggcgtat ttggaatcgc tgcaatagcg ggacgttgga agttggaacg
     4141 gttcgctcca atttctaatc tctaacttcc aatgaggtag aaaggacaat cggatatggc
     4201 aactgctcaa gcggcaatgc cgcgaacact catcgtgaaa tcaggcctta aggcgtggct
     4261 gaccaccgtc gatcacaaga agatcggtat cctgtatatt ttgtcgtcgg tgttcttctt
     4321 catcgtcggc ggcgtgctgg cgttggtcat gcgcttggaa ctggcccagc ctggcctgcc
     4381 ggtggtctcg gccgaattct ataatcaagc gttcacgatg cacggcacca cgatgatctt
     4441 cttgttcatc atcccgatct ttgctggcat cggcaactat attgtgccgc tgcaagtcgg
     4501 cgcgcgcgat atggcttacc cgcgcattaa tgccttgggt ttttggatga tcgtcggcgg
     4561 cgcgttgatt ttgtacagca gtttcttagt tggcgcaccc aacgccgggt ggactggcta
     4621 cagcccgctg gccaacagca tcttctctaa atcggccggt acggatatgt ggatcctcgg
     4681 cttgctgctg atcggcacgt cgtcgctgtt gggtgcgatc aacttcattg tcaccattct
     4741 caacatgcgc gcgccgggta tgaccttgac gcgtatgccg ttgttctgtt ggtcgatgtt
     4801 gacgacttca ttgatgattc tcgcatcgac gcctgtgttg actggtgcct tcatcatgtt
     4861 gtatgccgat cggaatttcg gcacgcagtt ctttagcggc gcgggcagcg atccgctgat
     4921 gtggcagcac atgttctggt tctactcgca tccggcggta tacattatga ttctgccggc
     4981 gatgggcatc gtctcggaaa ttctgccggt cttttccggc aaaccgatct ttggttacaa
     5041 agcgattgcg tactccaccg ccgcgattgc gattctcggc ttcatggtct gggcgcacca
     5101 tatgtttgcg accggcatga gcccgatctt gaaagtgttc ttcatgaccg cgacgatgac
     5161 aattgcggta ccgacaggtg taaaaatctt taactggatt gcgacgatct ggcgcggtca
     5221 cttacgcttc catacgccgt tgttatttat cttcggcttc ttgtccatgt ttgtgatcgg
     5281 cgggctcagc ggcatgttca gcgccgtcgt gccggtcgat ctgcagatca ccgacactta
     5341 cttcatcgtc gcgcatttgc actacgtctt gttcggcggc agcgtcttcg gaatattcgc
     5401 cggactgtat tattggacgc cgaagatgac cggctggtta atgaacgaaa agttgggcaa
     5461 gctgcatttc tggctgatgt tcatcggcgc caacctggcg ttcttcccgc agcacttttt
     5521 ggggctggcc ggcatgccgc gccgttacgc ggactat
//
LOCUS       DASRTJ010000402         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_62941,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000402 DASRTJ010000000
VERSION     DASRTJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62941"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFF70_08115"
     CDS             <1..796
                     /locus_tag="VFF70_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY44698.1"
                     /translation="FPIYESMIRFVSAHAVPIQLKMENDFAFDLHDLEKAITPETRMI
                     VINSPGNPTGGVIKRSNLEKIAQLAIAHDLIVFSDEIYSRIIYDEDFFSIASLPGMQE
                     RTIILDGFSKTYAMTGWRLGYGVMPVHVAQAIARLITNSTSCVATATQIAGVTALTSS
                     QDSVDQMLAAFRQRRDVIVTGLNQIPGFKCLMPAGAFYAFPNIEGTGRKSKELADTLL
                     NEAGVATLSGTAFGSYGEGFLRLSYANSIENIQKALERIDTAVRNM"
     gene            802..1341
                     /locus_tag="VFF70_08120"
     CDS             802..1341
                     /locus_tag="VFF70_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HZY44699.1"
                     /translation="MEIPATPRGPQETKSIPIVKVVVAGDGSVGKTSLIRRYSEGMFI
                     ASRVMTIGVDFQTASVEVSGQKIKLSIWDIAGQDRFGSFRGSFYRGARAVALVYDVTD
                     RLSWDNLPRWQAEIAKVAPAARYVVVGNKIDLDRSIPREEAEQFASNQGFPYIETSAL
                     TEAGVKEFFNTLGRLATIR"
     gene            1421..1630
                     /gene="rpsU"
                     /locus_tag="VFF70_08125"
     CDS             1421..1630
                     /gene="rpsU"
                     /locus_tag="VFF70_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZY44700.1"
                     /translation="MARVVVDDNESFESALKRFNKVVQGDRILSEVRRRRFFEKPSIV
                     RKRKKAAKVRKSRRQTLKFLRSSNL"
     gene            1703..2371
                     /locus_tag="VFF70_08130"
     CDS             1703..2371
                     /locus_tag="VFF70_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HZY44701.1"
                     /translation="MAPTLDSLILAAEQEGSESLKQGPTRLRWTKMPLQVGDRAPDFD
                     LLDMIGKSFQLQMSWQDRPALVLFWRHNGCSCGIDRARRLKEEYNSYLEAGANVVIVG
                     QSEPERAAQYAQLHSVPCPILCDPTRMVYETYGLLEGKPSQVLFDAPADLLRCELNAV
                     EKFIQSREGTARAIVDSPWQLPGEFVVDRSGIIRLAYRYQYCEDWPNPLVLIAALREA
                     AGQL"
     gene            2392..2844
                     /locus_tag="VFF70_08135"
     CDS             2392..2844
                     /locus_tag="VFF70_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZY44702.1"
                     /translation="MKMNVKEQLQADLKTALLNKDELRKNVIRFTVSAIKNAEIAKPG
                     HELSDADLLALLTTEVKRRKDTISELEKVDRPELLKEEKAQLEVLMNYMPKQLSRAAI
                     VETAQKVIAEVGASGPQATGQVMKRLMADLKGKADGKLVSEVVNELMK"
     gene            complement(2943..>4984)
                     /locus_tag="VFF70_08140"
     CDS             complement(2943..>4984)
                     /locus_tag="VFF70_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HZY44703.1"
                     /translation="PIMALGSGQASNDGTLAMTVAYPTDPRWAKLIKVDVIVQYATSG
                     AYYVTSINVQPHIATATSTVTPIPSATSTSIPPTKTPTRVPPTSTRVPPTVTPMVITD
                     WRGEYYPNQTLTGAPAVVRNDKDVNFDWNVGSPFMFIPADYFSARWTRSLLFAAKTYR
                     FTVRADDGVRLWIDGSLIIEEWHSAAPSAYARDVTLGAGWHAIRLEYYEATGNAIVQF
                     KIEEVQIYPDWKGEYFNNISLSGAPSVTRNDAAVNFDWGTSVPAQGLPKDNFSARWTR
                     SIQFAAKTYRFTVKADDGVRVWIDNNLIIDEWHGASAGSFSRDVALTAGAHAIRIEYY
                     EATGNANIQFKFEEAQTFSAWKGEYFNNPALSGLPIVTRNDIAVSFDWGNGAPAQGLA
                     ADKFSVRWTRTINFASGLYRFSLRADDGVRFYIDGSLLIDQWHDSSLTLYSREVNLGV
                     GLHILTIEYYENTGGASIYLTYQPVEDITKWQGEYFANDHWQGYPTLIRNDDHLDFDW
                     VSGSPDRLLPPDHFSVRWTRSINLDAGTYRFDMTVDDGVRFTIDNVVVLDQMHESAET
                     TYSVNRALTAGSHAFKIEYAEYAGQARFAWSRNLISSPTPTFTPTATPSRTPASTPTS
                     TPTSTPTLTPTPTATSTPTATPTTTATATATPTATPTMTPTLTATATLTSTATPP"
BASE COUNT         1160 a         1331 c         1393 g         1100 t
ORIGIN      
        1 ctttccaatt tatgaaagca tgattcgttt cgtcagcgcg catgccgtgc cgatccagtt
       61 gaagatggaa aacgacttcg cgttcgatct tcacgatctg gaaaaagcga ttacgccaga
      121 gacgcgcatg atcgtcatca actcaccggg caacccgacc ggcggggtga tcaaacgatc
      181 gaatctggaa aagatcgcgc agctggcgat cgcgcatgat ttgattgtgt tctcggatga
      241 aatttacagc cgcatcattt acgacgaaga ttttttcagc atcgccagtt tgcccggcat
      301 gcaagagcgc acgatcatac tcgacggctt ttccaagaca tacgcgatga ccggctggcg
      361 actcggctac ggtgtgatgc cggtgcatgt ggcccaagcg atagcccgcc tgattaccaa
      421 ttcgacttca tgcgtcgcca ccgccacgca gatcgccggc gtgacagcac tgaccagttc
      481 gcaagacagc gtcgatcaaa tgctggcagc ctttcgtcaa cgccgcgatg tgatcgtcac
      541 aggcctgaat cagattccgg gcttcaaatg cttgatgccc gcgggcgcat tctatgcgtt
      601 ccccaatatc gaaggcactg gccgcaaatc caaagaactg gcggacactt tattgaatga
      661 agccggtgtc gccacgctgt ccggcacagc ttttggatca tatggtgaag gctttttacg
      721 attatcctac gccaactcga tcgagaatat ccagaaagcg ttggaacgga tcgatactgc
      781 tgtcaggaat atgtaatccg attggaaatc ccagcgactc cgcgcggtcc acaggaaacg
      841 aaatcaatcc cgattgtcaa agtcgtcgtg gctggtgatg gctccgtcgg caagaccagt
      901 ttgatccggc gttacagtga agggatgttt atcgcctcac gcgtgatgac catcggcgtg
      961 gattttcaaa ccgccagcgt cgaggtcagc gggcagaaaa tcaaactatc gatctgggac
     1021 atcgccggtc aggatcgctt cggctcattc cgcggctcgt tttatcgcgg cgcgcgggcc
     1081 gtggcgctag tctacgatgt caccgatcgg ctctcgtggg ataacctgcc gcgttggcag
     1141 gccgagatcg ccaaggtcgc acccgcggcc cgctacgttg tcgttggcaa taagatcgat
     1201 ctggatcggt ccattccgcg cgaagaagcg gaacaatttg cgagtaacca gggttttcct
     1261 tacattgaaa ccagcgcctt aacggaagcc ggcgtcaaag agttcttcaa caccttgggc
     1321 cgcctggcca ctattcgtta gttgacaagc atcggcggcg cggttataat ctccgtcgtt
     1381 gcacacaagg tagtatgtcc ggagagcgag gtgaattttc ttggcacgtg tagttgtaga
     1441 tgacaacgag tcgtttgagt cagcgctcaa acgcttcaac aaggtcgttc agggcgatcg
     1501 gattctgagt gaagtgcgcc gtcggcgctt cttcgaaaag cccagcatcg ttcgcaaacg
     1561 caagaaggcg gccaaagttc gcaagagccg ccgccagacg ctgaagttct tgcgatcgag
     1621 caacctgtga gactttcctc aacgcgcact tcggtgcgcg ttgttgttta tagaataaca
     1681 accactgaga gggaggaaat atatggcacc aaccttggat tctcttattc tggctgctga
     1741 acaagaaggc tcagagagcc tcaagcaggg gccgacccgc ctgcgctgga ccaaaatgcc
     1801 actccaggtt ggcgatcgtg cgcccgattt tgatttattg gacatgatcg gaaaatcatt
     1861 tcagttgcag atgagttggc aggaccgacc agcccttgtc cttttttggc ggcataacgg
     1921 ttgcagctgc ggaattgatc gcgctcgccg cttaaaagaa gaatataaca gttatttgga
     1981 ggccggagca aatgtcgtca ttgtaggaca aagcgagcca gaacgcgccg ctcaatacgc
     2041 gcagctgcat agtgtcccct gtccgattct atgtgatccc actcgaatgg tttatgagac
     2101 atacggtctc ttagaaggca agccttcaca agtattgttc gatgcacccg ctgatctgtt
     2161 aaggtgtgag cttaacgccg tggagaaatt catccaatca cgggagggga ctgcacgtgc
     2221 gattgtcgac agtccgtggc agctgcccgg cgaattcgtc gtcgatcgat ccgggatcat
     2281 ccggttagcg tatcgttacc aatattgcga agactggccg aatccgctgg tcttgatcgc
     2341 ggcgctgcgc gaggccgccg ggcagctata gcccatgact ctgtggagtg aatgaagatg
     2401 aacgtcaagg aacaactgca agccgatttg aaaaccgccc tgctcaacaa agacgaactg
     2461 cgcaagaacg tcattcgttt tactgtctcg gccatcaaga acgcagagat cgctaaaccc
     2521 gggcatgaac tatctgacgc ggacctgctg gcgctgctga ccaccgaagt gaaacgccgc
     2581 aaagatacca tatccgaact cgaaaaagtc gatcggcctg agctgctgaa agaagaaaaa
     2641 gcgcagcttg aagtgctgat gaactatatg cccaagcagt tgagccgcgc agcgatcgtc
     2701 gagacggcgc agaaagtcat cgctgaagtc ggcgctagcg gtccacaagc caccggccaa
     2761 gttatgaaac gcttgatggc tgatctgaaa ggcaaggccg acggcaaact cgtcagcgaa
     2821 gtcgtcaacg aattaatgaa gtagagattt tccggcggaa tgtctcgcta acagaatact
     2881 ggcgcgcatc aaatttttgc gcgccagtat ttttggttgt gtcagcgttg ccagtaaaaa
     2941 cgctaaggtg gtgtagccgt cgatgttagc gttgcggtag ccgtcagtgt cggtgtcatg
     3001 gtcggcgtgg ctgtcggagt cgccgttgcc gtagctgttg ttgttggcgt ggccgtcggt
     3061 gttgatgtcg ccgtcggtgt cggcgtcagc gtcggagtgg aagtcggtgt cgatgttgga
     3121 gtcgaggctg gcgtacggct cggagtggcc gtcggcgtga aggtcggcgt cggcgatgag
     3181 atcaaattgc ggctccaggc gaaacgcgct tgacccgcgt attcagcgta ttcgatcttg
     3241 aacgcatgtg atccagccgt tagcgcacgg ttaaccgaat aggttgtctc cgccgattcg
     3301 tgcatttgat caagcaccac cacattgtcg atggtgaaac gcacgccatc atccaccgtc
     3361 atgtcgaagc gataagtgcc cgcatccaga ttgatcgatc gggtccaccg caccgagaaa
     3421 tgatcgggag gcagcaaccg atcgggtgag ccgctgaccc aatcgaaatc caaatgatcg
     3481 tcattgcgga tcaacgtcgg ataaccctgc caatgatcgt tcgcaaaata ttcgccctgc
     3541 catttggtaa tgtcctcgac gggttgatag gtgagataaa tactcgcgcc accggtgttc
     3601 tcgtaatatt cgatcgtcaa gatatgcagg cccacgccca gattcacttc gcggctatac
     3661 agcgtcagtg aagaatcgtg ccactgatcg atgagcaagc tgccatcaat gtagaagcgc
     3721 acgccgtcat cggcgcgcag actgaaacga tacaagccac tcgcgaagtt gatcgtgcgc
     3781 gtccagcgca ccgagaattt atcagcagct agtccttggg cgggcgcgcc attgccccaa
     3841 tcaaaactca ccgcgatgtc attgcgcgtt acgatcggca gtccgcttaa cgctggatta
     3901 ttgaagtact cacctttcca ggctgaaaat gtctgtgctt cttcaaattt gaattggatg
     3961 ttagcattgc cggtggcttc atagtattcg atgcggatgg catgcgcgcc ggcagtcaac
     4021 gccacatcgc gcgagaatga tccggccgag gcgccatgcc attcatcgat gatgagattg
     4081 ttatcgatcc acacccgcac gccatcatca gccttcacgg taaaccgata ggtcttcgcg
     4141 gcgaactgga tcgatcgagt ccagcgcgcc gagaagttat ctttcggcaa accttgcgcc
     4201 ggaacgctcg tgccccaatc gaaatttacg gcggcatcat tgcgcgtcac gctcggcgca
     4261 ccgctcaaag aaatgttgtt gaaatactcg cccttccaat ccggataaat ctgcacctct
     4321 tcgatcttga attgcacgat cgcattgccg gtcgcttcat agtattcgag gcggatggca
     4381 tgccagcccg cgcccaacgt cacatcacgc gcgtaagcgg acggcgcggc cgaatgccac
     4441 tcttcgataa tcaagctgcc atcgatccac aagcgcacgc catcatcggc tcttactgta
     4501 aaccgataag tcttcgcggc gaacaagagc gatcgagtcc aacgcgcgga gaagtaatca
     4561 gctggaataa acatgaacgg cgagccgacg ttccaatcga agttcacatc tttgtcattg
     4621 cgcacgacag ccggagcgcc ggtcaacgtt tgattcggat aatattcgcc gcgccaatcg
     4681 gtaatgacca tcggcgttac cgtcggcggc acgcgcgtcg aggtgggcgg aactcgcgtc
     4741 ggagttttcg tcggcgggat cgaagttgaa gtcgcggacg ggatcggtgt cacggtcgag
     4801 gtcgccgtgg cgatgtgcgg ctgcacgttg atcgatgtca cgtagtaggc gccgctcgtg
     4861 gcatactgta cgatcacgtc gactttgatc aacttggccc agcgtggatc ggtgggataa
     4921 gctaccgtca tggcgagagt tccatcgttc gatgcttgac cggaacctaa agccataata
     4981 ggat
//
LOCUS       DASRTJ010000403         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_162093,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000403 DASRTJ010000000
VERSION     DASRTJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162093"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFF70_08145"
     CDS             <1..849
                     /locus_tag="VFF70_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44704.1"
                     /translation="AKAGLIIPPDYGDRVAHGEQAQVAFVIDGSDPTIAATALSAATL
                     IGQTKATAVLRATLETRGQSGAINQPVEVRTQVWYNPDLVSAYFMIPGLIGMILQYLA
                     VLLTSTAVVRERERGTIEQLIITPIRSWELIVSKLMPYALISFLDLLEVLVIGTLWFK
                     VPINGSVALLLILSGLFLVTTLGLGLLISTIASTQFEAMLIAIMIQLPSIFLSGFFFP
                     IAAMPPFLQAVSYLVPLRYFLIVVRAVQIKGVGLPAVSNEVIALIIFGVVVMGAAAIR
                     FRKRLD"
     gene            1014..1529
                     /locus_tag="VFF70_08150"
     CDS             1014..1529
                     /locus_tag="VFF70_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY44705.1"
                     /translation="MTAAIDFESLVTKHSAEIFAYIWRMLRDTLDAEDCLQETFLRAY
                     RAYPRLPIDANHRAWLYKIATNTARTHIQRRTRDQRRVSDLDPELQSDRSSPLDGLDQ
                     RERLAAVTRAVEALPYQQRAALVMKKYQELSYEAIASALNCSEDAARANVYQALKKLR
                     AQFTEELEVSR"
     gene            1526..2203
                     /locus_tag="VFF70_08155"
     CDS             1526..2203
                     /locus_tag="VFF70_08155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZY44706.1"
                     /translation="MTPQHAKVWLKDNERLIESNAVTQSLDALYAAGPNAPSIARALA
                     KLRATLAREKSNVIYYDMLPNTAVGAMFAAVSEHGLIAVDFGIDEHAFVDRVQRKTRS
                     VLVRSPREVKQVLKQLNDYLIGKRKAFDLSIDYSVLTDFQKKVLVAALKIPRGQVLTY
                     ADVARKIGRPRASRAVGQALGHNPMPIVIPCHRVLGSDGSLHGYSGGGGLKTKAWLLK
                     LEGAQLH"
     gene            2292..3215
                     /locus_tag="VFF70_08160"
     CDS             2292..3215
                     /locus_tag="VFF70_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY44707.1"
                     /translation="MKAREWGIFGLLGLIWGSSFLWIKIAVAEVGPFVLVSFRLLFGL
                     VGLVTIMFLTKRAFPRDRKMWLRFLFMGVVNTAIPFTLISWGETKIDSSLASILNGSV
                     PLFTIIIAHFWLHDEKITFGRMAGLIVGFIGVIVLVSGDLNPEGLHGDLIGQLAVLLA
                     AISYATAITFSRKYLRGSNPLLQAASTLLIADSIMWLVTPFAERPMILPALPITWLAI
                     AWLGLLGTCTAYLMFFSLINAWGATRASLVTYVFPVIGLILGIIFLKEEADWRLLVGS
                     ALVIVSVLAVNFNQLFRSAPATAPASALASK"
     gene            3219..3548
                     /locus_tag="VFF70_08165"
     CDS             3219..3548
                     /locus_tag="VFF70_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY44708.1"
                     /translation="MDAHELTDLIGQLDRSGQLYHEFLRVPSMSAGLYRLRAGAIDPQ
                     SPHTEDEIYTVISGRGSIRIGVEDRSVIAGSIVFVPATIEHRFHSIEEDLIIFVFFAP
                     AEYSQTK"
     gene            3545..4288
                     /locus_tag="VFF70_08170"
     CDS             3545..4288
                     /locus_tag="VFF70_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZY44709.1"
                     /translation="MTTPRSEFFGGVKAELPILLGVVPFGLIYGVLAIGAHIPIDVAL
                     FMSSIVFAGSSQLVGTQLISAGSPLIVIVLTTFIVNLRHALYSASLAPQVKHLPRRWK
                     WLLAYLLTDEAYAVTITHYHAASELSHKHWFFLGSGLTLWTSWQISTAIGVFLGAQVP
                     ASWSLDFTLALTFIALTVPRLKDRPSAAAAISAGVVAVLGNGLPYKLGLMLAALIGVV
                     VGLITESQVRRAEISESFIMPIRSPDSKS"
     gene            4285..4617
                     /locus_tag="VFF70_08175"
     CDS             4285..4617
                     /locus_tag="VFF70_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015910111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZY44710.1"
                     /translation="MNNYWLIIIGMGLVTYGIRLSAIALLGQREFPLIIQRALRFVPP
                     AVLSAIIFPELFQHSGQFDLSLSNARLLAGVLASIVAWRSKNVLATIAAGMIALWLLQ
                     AVLAASGS"
     gene            4686..5837
                     /locus_tag="VFF70_08180"
     CDS             4686..5837
                     /locus_tag="VFF70_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZY44711.1"
                     /translation="MTKRSRARGAQAMTTLGIIVVGAAILLYFFKPDLFAQLTGQAPA
                     PTPVGQLPSSPANTGSQSIQVFFTTPKFPDNAADHHGGIDVHLTDFINTAQTSLDMAI
                     YQFDLAGATQALLDAKQRGVTIRVATDIDTLEDDHENQSFLKLKQAGIPVISGNRDGI
                     MHNKFIVVNGQAVWMGTWNFTDDDTYRYNNVGLLIQSAELAHNYTAEFEKMFVDKNFG
                     NRKQTRAVQHELTIDGLHVENYFTPADNATSSIVTRLKQAKTSIHFLAFSFTEDSIGQ
                     AVIDRSKAGVEVRGVFENTGSDTRFSEFGRMKRAGLDVLQDGNPYLMHHKIFILDGQT
                     VIVGSFNFSDNAQNQNDENLIIVDSPALAQTFEAEFLRVYAIAQNPPNK"
BASE COUNT         1245 a         1518 c         1692 g         1496 t
ORIGIN      
        1 gccaaggccg gtttgatcat tccgcccgat tacggtgatc gcgttgcgca tggcgagcag
       61 gctcaggtcg cctttgtgat cgatggctct gatccgacga ttgcggcgac cgcgttgtcg
      121 gcggcgacat tgatcgggca gacgaaggcc acggcggtgt tgcgcgccac gcttgaaacg
      181 cgcgggcaaa gcggcgcgat caatcagccc gttgaagttc gcacgcaggt ctggtacaac
      241 cctgatctcg tcagcgcgta ttttatgatc cccgggttga tcggcatgat cctgcaatac
      301 cttgccgtgc tgctgacctc aacggccgtg gtacgggaac gcgagcgcgg cacgatcgag
      361 cagctgatta tcacgccgat tcgatcgtgg gaattgatcg tcagcaaatt gatgccgtat
      421 gcgctgatct cgtttctcga tctactggaa gtgttggtta tcggcacgtt gtggttcaaa
      481 gtgccgatca atggcagcgt ggcgctgctg cttattttgt cgggcttgtt tctcgtaacc
      541 acactgggat tgggtttatt gatctcgacg attgccagca cgcagtttga agcgatgctg
      601 atcgcgatca tgatccagct gccctcgatc ttcttgtcgg gtttcttttt cccgatcgcc
      661 gcgatgccgc ccttcctgca agcagtcagt tatctggtgc cactgcgtta tttcttgatc
      721 gtcgtgcgcg cagtgcagat taaaggggtg ggcttgcccg ccgtgtcgaa cgaagtgatc
      781 gcgctgatta tctttggcgt ggtagtgatg ggcgcagcgg caatccgttt ccgcaagcgt
      841 ctggattgaa ggccaatcta accgcggaca cttgctacgt gcgctgcagt gtaggtgtga
      901 cacaaatgac acagatttgg tttttgatag tgaaagctgt gtcagctgtg gtttcttttc
      961 acgaaatccc ggcaacgtgg ttatattagt cagacttctg aagcgagcgc ttgatgacag
     1021 ccgcaatcga ttttgagagt ttagtgacta aacacagcgc ggaaattttt gcctatattt
     1081 ggcggatgct gcgcgacacg ctggatgccg aagattgctt gcaagaaaca ttcctgcggg
     1141 cctaccgtgc ttatccgcgc ctgccgatcg atgcgaatca ccgcgcctgg ttgtacaaga
     1201 ttgcgacgaa caccgcgcgg acgcacatcc aacgccggac acgggatcaa cgtcgagtga
     1261 gtgatcttga tcctgaattg cagtctgatc gttcctctcc gctcgatggt ctggatcagc
     1321 gcgaacgctt ggcggcagtt acccgcgcag tggaagcatt gccctatcaa caaagggcgg
     1381 cattggtgat gaagaagtat caagagttga gttatgaggc aattgcctcg gcgttaaatt
     1441 gcagtgaaga tgccgcacgc gcgaatgtgt atcaggcgtt gaagaaactc cgcgcacaat
     1501 ttacagaaga actggaggtt agtcgatgac tcctcaacac gctaaggtgt ggttgaaaga
     1561 taatgaacga ctgattgaat cgaatgcggt aacgcagtca ctcgatgcgc tatatgccgc
     1621 tggtccgaac gcgccatcga tcgcgcgagc actggctaaa ttacgcgcga cgttagcccg
     1681 ggagaagagt aacgtgattt actacgatat gctgccgaat acagccgtcg gcgcaatgtt
     1741 cgccgcagtc agtgagcacg gtttgatcgc cgtcgatttt gggatcgatg agcacgcctt
     1801 tgtcgatcgg gttcagcgca aaacgcgatc ggtgttagtt cgatcaccgc gtgaagttaa
     1861 acaggtgttg aagcaattga atgattactt gatcggcaag cgcaaggcat tcgatctatc
     1921 gatcgattac agcgtgctga ccgatttcca aaagaaagtg ctggtcgcgg cgttaaagat
     1981 tccacgcgga caagtcttga cctatgctga tgtcgcgcgg aagatcggtc ggccgcgcgc
     2041 gtcacgcgca gtcgggcagg cgttggggca caaccccatg ccgatcgtga tcccgtgcca
     2101 tcgcgtgctc ggatcggatg gatcgctgca cggttattca ggcggcggcg gcctcaagac
     2161 caaggcgtgg ctgttgaagc tggaaggcgc gcagctgcac tgatcaattg gattgccagg
     2221 ctaggcttgc gatgagacgc cgatagcaat ctgttacaat tcatttattg ccagacaaga
     2281 gaggatattt tatgaaggcg cgagagtggg gcatttttgg tttattgggt ttgatctggg
     2341 gttcgtcgtt tctgtggatc aagatcgcgg tcgccgaagt cgggccgttt gtcctggtgt
     2401 ccttccggct gttgttcgga ctggtcggtt tggtgacgat catgttcttg acgaaacgcg
     2461 cttttccacg cgatcgcaaa atgtggctca gatttttgtt tatgggcgtg gtgaatacgg
     2521 ccattccctt cacgctgatc tcgtggggcg aaacgaagat cgattccagc ctcgcctcga
     2581 tcttgaacgg cagcgtgccg ctgtttacga tcatcatcgc gcacttctgg ctgcatgacg
     2641 agaagatcac tttcggacgc atggcgggtt tgatcgtcgg cttcatcggc gtgattgtgt
     2701 tggttagcgg cgatctcaat cctgaagggc tgcacggtga tttgatcgga cagctggcgg
     2761 tgctgctggc ggccatcagc tacgccacgg cgatcacttt ctcgcgcaaa tatttgcggg
     2821 gctcgaatcc attattgcaa gcggcctcga cgttattgat cgccgattcg atcatgtggc
     2881 tggtcacgcc gttcgccgag cggccgatga tcctgcccgc actgccgatc acttggctgg
     2941 cgattgcgtg gctcggcctg ttgggcacgt gcactgcgta tttaatgttc ttctcgttga
     3001 tcaatgcgtg gggcgcgacg cgcgcatcgt tggtgaccta cgtctttccc gtgatcggct
     3061 tgatcctcgg tatcatcttc ttgaaagaag aagccgattg gcgcttgctc gtcggctcgg
     3121 cgctggtcat cgtcagcgtg ctggccgtca acttcaatca gttgtttcga tcggcgccgg
     3181 cgactgcacc tgcgtcagcg ctggcgagta aatgatccat ggatgcgcat gagctgacag
     3241 acttgatcgg acagctggat cgatcgggcc agctgtatca cgaatttctg cgcgtgccgt
     3301 cgatgagcgc cggcctatac cgattgcgcg ccggagcgat cgatccacaa tcgccgcaca
     3361 cggaagacga aatctacact gtgatcagcg gccgcggatc gatccggata ggtgtggaag
     3421 accgatcggt tatagctggc tcgatcgttt ttgtgcctgc cacgatcgag catcgtttcc
     3481 attcgatcga agaagatttg atcatcttcg ttttcttcgc gccggccgaa tactctcaaa
     3541 cgaaatgact acaccccgat cagaattttt tggcggcgtc aaagccgaac tgccgatatt
     3601 gcttggcgtt gttccattcg gcttgatcta cggcgtgctg gcgatcggcg cgcacattcc
     3661 gatcgatgtc gcgttgttca tgtcgtcgat tgtgtttgcc ggatcgtcac agcttgtcgg
     3721 cacgcaatta atcagcgccg gctcaccgct gattgtaatc gtgctgacga cgttcatcgt
     3781 gaatttgcgc catgcactct atagtgcctc acttgcgccg caggtgaagc acttgccacg
     3841 ccgctggaaa tggttgttgg cgtatctatt aactgacgaa gcttatgcgg tcaccattac
     3901 gcactatcat gcagccagcg aattgtcaca caagcattgg ttctttttgg gatccggtct
     3961 tacgctgtgg acctcgtggc agatcagcac tgcaatcggt gtttttcttg gcgcacaagt
     4021 tccagccagt tggtcgttgg acttcacgct cgctttgact ttcattgcct tgacggtgcc
     4081 tcgcctgaaa gatcggccga gtgcggcagc ggcgatctca gcgggtgtgg tagcggtgtt
     4141 gggtaatggc ttgccgtata aacttggctt gatgctggcg gcgttgatcg gagtggtggt
     4201 tgggttgatt actgaatctc aagtgagacg ggccgaaatt tctgaatcgt tcattatgcc
     4261 tatccgtagc cctgattcaa aatcatgaac aattactggc tgattatcat cggaatgggt
     4321 ttagtaactt acggcattcg cctgtccgcg atcgcgctgc tgggccagcg cgaattcccg
     4381 ttgatcattc aacgggcact gcgttttgtg ccgcccgcgg ttctgtcggc gattattttt
     4441 cccgaactgt ttcagcacag tggccaattc gatctatctc tgtcaaacgc gcgcttgctg
     4501 gcgggcgtgc ttgcatcgat cgttgcctgg cgatcgaaga atgtgctggc gacgatcgca
     4561 gcgggcatga tcgcgttgtg gttattgcaa gcggtgctgg cggcgtccgg ttcataaagt
     4621 gtgtttgtca gtctcttctt atcgcggtat gatcgtcggc actccacacc cgaaaggttt
     4681 tcacaatgac caaacgatcg cgcgcccgcg gcgcacaggc gatgaccacg cttggcatca
     4741 ttgtcgttgg ggcagcaatt ctcctgtact tcttcaaacc ggacttgttc gctcaattaa
     4801 cgggtcaagc acctgcgcct acgccagtcg gtcaacttcc atcatcgcct gccaacacgg
     4861 gtagccagtc gattcaagtt ttcttcacca cgccgaaatt tcccgacaac gccgccgatc
     4921 atcacggcgg catagatgtt catctaacag atttcatcaa tacggctcag accagtcttg
     4981 atatggcaat ctatcaattt gatctagccg gcgcaactca agcgctgctc gatgccaaac
     5041 agcgcggcgt caccattcgg gttgcgacag acattgatac tctagaagac gatcacgaga
     5101 accagtcgtt tctgaagctt aaacaagccg gcattccggt catcagtggc aaccgagacg
     5161 ggatcatgca taataaattt atcgttgtga atggtcaggc ggtctggatg gggacgtgga
     5221 actttaccga cgacgacact tatcgttaca acaatgtcgg gctgctcatt cagtcggccg
     5281 aattggcgca caactatacc gccgaatttg agaagatgtt tgtcgataaa aatttcggca
     5341 accggaaaca aacccgcgct gttcagcacg agttgacgat cgatggactg catgtcgaga
     5401 attacttcac gccggccgat aatgccacca gctcaattgt gacccgcctg aagcaagcca
     5461 agacctcgat ccattttctg gccttctcat ttaccgaaga ctcgattggg caagcggtaa
     5521 tcgatcgatc taaagcgggc gtggaagtac gcggcgtgtt tgaaaatacc ggttcggata
     5581 cgcgtttcag tgaatttggc cgtatgaaac gagccggcct ggatgtattg caagacggca
     5641 atccgtattt gatgcatcac aagattttca ttcttgacgg ccagacggtg atcgtcggct
     5701 cgtttaactt ttcggataac gcgcagaacc agaacgacga aaacctgatc atcgtcgata
     5761 gtccggcgct ggcccagacg ttcgaagccg aattcctgcg ggtgtatgct atcgcgcaaa
     5821 atccgccgaa caagtgaggc gagatgatca gatttgaaaa taaaattgaa atgagtttgc
     5881 agcagctgct ggaagacgag cggctgcgat ccaacttaat cgataccgaa gcgagtctgc
     5941 tgtttgattg g
//
LOCUS       DASRTJ010000404         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_252175,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000404 DASRTJ010000000
VERSION     DASRTJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252175"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFF70_08185"
     CDS             <1..843
                     /locus_tag="VFF70_08185"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HZY44712.1"
                     /translation="AINSLYPTNSTNTEAGLHLGYDLAYRNYRADAINRVILASDGLA
                     NVGDTNPDTLSQYAHDYYGRGVYLSTVGVGHGGYDDHFLEQLADKGNGSYSFLDSPAA
                     ADRIFSQDVNGTLQAIAKDAKIQVDFNANSVRSYRLIGYENRAVADQNFRNDTVDAGE
                     VGAGQNVTALYEVQLNSEGSTDALVVRVRYEDPSTHAIAEVSQPFQRSDFQRDFARTS
                     PRFQLASAVVQFAEQLRRDPWTQSSGLQDVLALAKIVQVQLPYDNDVNEFVHLVEQAN
                     YLTQ"
     gene            complement(998..1882)
                     /locus_tag="VFF70_08190"
     CDS             complement(998..1882)
                     /locus_tag="VFF70_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44713.1"
                     /translation="MATEANIKNRRQYGLRHAANGLKHLLAVIRAQIQSGLKAIAKAS
                     LRQVWVLNSRIGPTVSLPFAFKLIVVLVGYEEKRMQNIEPLIRSALKCTFVEKVIASN
                     NNPRLHIRDWVKITNPRLKLIDQIVPRGSGYRWVLIEQESANFILTIDDDILLCPQQM
                     ACLFAQLVAKPDVPHGLAGHADSGYIQCQDAAVNALYQVYAVTALQVKLYFERINEIR
                     VLDQAVFDSIEYFGDDLVISAVGASHPLIHNVGFVLRCTTASQIGVATFTQPDFEAKR
                     VAIQKCLAATSVKSEAGR"
     gene            complement(2164..3228)
                     /locus_tag="VFF70_08195"
     CDS             complement(2164..3228)
                     /locus_tag="VFF70_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44714.1"
                     /translation="MTTTYRIALGRWAVQDELAESIGDELLGLGHSVIPFLFNAIIPR
                     QCDVILTFAPHGRWWPIARQIRPRSTTTSRPIFVHWNFEGQPSLRFKPQAAFGLGAIR
                     AWMDRLNDSSLAWVRRGVQPSPAAFINRHLHKFRYLGEYEFAFRQGILDILIESSEIF
                     AARFQRRGLLVQVVPWGTSKRWYQTLNLKRDIDVLWLGQRRTRHRSDLLDRLRRELAA
                     AGIHLHIVDNIEHPFVYAAERNQLFNRAKITLSLLPTPNDNSFPYRFHLAAGNRSVVV
                     SDPLMRHTARYEAGRDYIAAPIESLAQTIRYYLQHEAERAEIAENAFRLVTTDLTLRN
                     SLAAVVEAIDGFQSSLKGTI"
     gene            3370..4278
                     /locus_tag="VFF70_08200"
     CDS             3370..4278
                     /locus_tag="VFF70_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZY44715.1"
                     /translation="MQTSVSTSRRGYGLVLIAVSLWATLGIFYTALLQLGFSRVSIAF
                     IRCTIAAILLGSRLIVNDRARLKVAWHDLPLLIAFGVIGIAVFYVVQIISVESAGVAV
                     TWVMLYTAPIWVVLFSAIFLHERITQPKMIAIALSLAGCALVVKIYQGAAINLSGLLA
                     GLATGLTYAAYILFSKAASARGYNTWTFMLYGLLFGAICLLPLQSIDDLTRSIAAPGA
                     ILWLILLCLGPTFAAATIFITGLKYVRASNASIVATIEPVLATLLALIFLHESLEPLQ
                     LIGAGLILSAVIVLLRSPDRSERVAI"
     gene            complement(4366..4518)
                     /locus_tag="VFF70_08205"
     CDS             complement(4366..4518)
                     /locus_tag="VFF70_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44716.1"
                     /translation="MKLQKKWGLLLLGILAILTGLSSFLPFLAGLGVVWAILWIVAGI
                     LLVLDM"
     gene            complement(4611..5405)
                     /locus_tag="VFF70_08210"
     CDS             complement(4611..5405)
                     /locus_tag="VFF70_08210"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZY44717.1"
                     /translation="MKRRVYRGGALLALIAAIVVIAIILTENSLRGQLTATATSWTDT
                     GRWLAAALGGYLLGSIPFGFIIVAVLHERDIRDSGSGRTGGTNAMRASGMGAAAFTVL
                     GDLLKGFAGVALAQLIFPTSAWAAVFGGWGVLLGHNASIYLGFRGGAGTAPSMGVAGA
                     LWFPSLLFTLPFLPIGLLVIGVASLTSLIISGAIIVVFVIRAATGYGPWEHVVYGLGA
                     LILVAFALRPNIKRMLNGTEPIVGPRARRIEKRAAQSANPAARSGS"
     gene            complement(5483..>5880)
                     /locus_tag="VFF70_08215"
     CDS             complement(5483..>5880)
                     /locus_tag="VFF70_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZY44718.1"
                     /translation="GREAVIFSLALVNQMLAHVLSDPSQERCGLLAGEAGEINRVLPV
                     PNALRSPSAYRMDGPEFIEAMQTCDFDPIGIYHSHISGPATPSATDIAEANYPDSIYF
                     ILSLDVSPPSVRGFKIVNRRVSEVDITLN"
BASE COUNT         1432 a         1641 c         1502 g         1305 t
ORIGIN      
        1 gccatcaaca gtctgtatcc gacaaactcc accaacaccg aagcgggcct gcatctcggc
       61 tacgatttag cctaccgcaa ttaccgcgca gacgcgatca accgcgtcat cttagccagc
      121 gatggcctgg cgaatgtcgg cgatacaaat ccggatacgc tgtcacaata tgcgcatgat
      181 tattacgggc gcggcgtcta tctttcgaca gtgggcgtcg gccacggcgg ctatgacgat
      241 catttcttag aacagctggc cgataagggt aacgggagct atagcttcct cgatagtcca
      301 gctgcggccg atcggatctt cagccaggac gtgaatggca cgctgcaggc gatcgcaaaa
      361 gatgccaaga ttcaagtcga tttcaatgcc aactccgttc gcagctatcg cctgatcggt
      421 tatgagaatc gcgccgtcgc cgatcagaat ttccgtaacg acacggtcga tgcgggcgaa
      481 gtcggcgcag gccaaaacgt cacggcgttg tatgaagtgc agctcaattc cgaaggcagc
      541 actgatgcct tagtcgtgcg tgtgcgttac gaagatccga gcacacatgc catcgcagaa
      601 gttagccaac ctttccagcg atctgatttc cagcgcgact tcgcccggac ttcaccccgc
      661 ttccaattgg cgtcggccgt cgtgcaattc gccgagcagc tgcggcgcga tccatggacg
      721 caaagttcgg ggctacaaga tgtgctggcg ctagccaaaa ttgtgcaggt ccagctgcct
      781 tacgacaacg acgtaaatga atttgtgcat ctggtggaac aagcgaatta tttgacccag
      841 tagtcacagc caccgtccgg cggctcatag ccgccggacg gtttaggtta acgcagcaac
      901 tgcgacgcga tcaacgtttc cgtggcaaca cgaatgctct tggactgcat caccaacaca
      961 gcgacgcgcc aatcgcagcc aaatctgtaa tgccctctta tcgcccggcc tccgatttta
     1021 cactggttgc agccaggcat ttttggatcg caacgcgttt ggcttcaaag tctggctgag
     1081 taaatgtcgc caccccgatt tgactggcag ttgtgcagcg cagaacgaag ccgacgttgt
     1141 gtatcagcgg atgactcgct cctacggcgc taatcactaa gtcatcgccg aaatattcaa
     1201 tagagtcaaa tactgcttga tcaagcactc gaatctcatt tattcgttca aaatacagct
     1261 tcacttgaag agcagtgacg gcatacacct gatacagagc attcactgcg gcatcttgac
     1321 attgaatata gcctgagtcc gcatgcccgg ccaagccatg cgggacgtcg ggcttcgcca
     1381 ccagctgcgc aaacaggcag gccatttgtt gcggacacag caaaatgtca tcgtcgatgg
     1441 ttagaatgaa attagcggat tcttgttcga tcaaaaccca acggtagcct gatccgcgcg
     1501 ggacaatttg atcgatcagc ttcagacgcg gattggtgat cttgacccaa tcacgaatgt
     1561 gcaatcgcgg attattgttt gaggcaatta ccttttcaac aaacgtgcat ttcagcgctg
     1621 atcgaatcag cggctcgata ttctgcatcc gcttttcttc atatccgacc aggacgacaa
     1681 tcaatttaaa agcgaacggc agagacacgg ttggcccaat tcgactattc aacacccaga
     1741 cttggcgtag actagctttt gcaatggcct tcagaccgga ttgaatctgg gcgcggataa
     1801 ctgccagcaa atgcttgaga ccgttcgccg cgtggcggag accatactgc ctgcgattct
     1861 tgatattcgc ttcagtcgcc atgagcgtgc ttaatgatca tctaattcgt tcgataaact
     1921 tgaagaagag aggtagagaa tggtcatact ttagatacgc gttgtgcgac attccaggtt
     1981 gaataaacta cagctggctt tctctggagc ccacaggcag ggtagtgctc gtgtcttcac
     2041 atactgagcc tggcaccgca gcgtcctgcg ttggtgcagc cgctggactt accacagagc
     2101 agtgaaatag gccggtcaaa aaagttgccg cgaccgcaat aagttcgaga atttgtatga
     2161 gagtcaaatt gttcctttca aagaagattg aaatccatct atcgcttcga cgacggctgc
     2221 caggctatta cgcaatgtca gatcagtcgt gaccaaccgg aaagcgttct cggcaatttc
     2281 agcccgctca gcctcatgct gcaaataata ccggatcgtt tgggctagag attcaatcgg
     2341 agcagcgata taatcgcgtc cggcctcata tcgagcagta tgccgcatga gcgggtcgga
     2401 caccaccacc gatcgattgc cggctgccag atgaaaacga taaggaaagc tattgtcatt
     2461 cggcgtcggc agcaggctca gtgtaatctt ggcgcggtta aacaattgat tgcgttccgc
     2521 cgcatacacg aacgggtgct cgatgttgtc gacgatatgc aggtgtattc cagcagccgc
     2581 cagttcgcgg cgaagccgat cgagcagatc acttcgatgg cgtgtacggc gctgccccag
     2641 ccacagcaca tcgatatcgc gcttcaaatt cagcgtttga taccagcgct ttgacgtacc
     2701 ccacgggacg acttggacta gcaaccctcg acgctgaaaa cgcgccgcaa atatttcgga
     2761 tgattcaatg agtatatcca agatgccctg acgaaaggca aattcatatt cgccaagata
     2821 acgaaactta tgcagatgcc gattaataaa cgccgctggt gagggctgca ccccgcgccg
     2881 cacccacgcc agcgaggaat cgttcaaccg atccatccac gcccggattg cgcccagccc
     2941 aaaagcagct tggggcttga accttaagct cggctggcct tcaaaattcc agtgcacaaa
     3001 gatcgggcgg ctggtcgtgg ttgatcgagg tcgaatctgg cgcgcgatcg gccaccagcg
     3061 accgtgcggc gcaaaggtga ggatcacatc gcactggcgc gggatgattg cgttgaacaa
     3121 gaagggaatg accgaatggc ctagtcccaa cagctcatcc ccgatcgact cggccaattc
     3181 atcctgcaca gcccaacgtc cgagtgcgat tcgataagtg gttgtcacgc taatcgaagt
     3241 agctgaaata gtctgaaaca gcggggaacc ctaaacacga gcttgttcct taacgcgagt
     3301 gctgatcgtt gtgccagtag agtataaatc acatccacgg atacttcaat cgtcgattat
     3361 aatgcgcgga tgcaaacttc tgtttcaact tccagacgtg gctatggact ggtcttgatc
     3421 gccgtttcat tgtgggcaac gctgggtatt ttttataccg ccctgcttca attaggtttt
     3481 tcccgcgtca gcattgcctt cattcgttgc acgatcgccg ccattctctt agggtcgaga
     3541 ttgatcgtga acgaccgcgc gcggctcaaa gtggcttggc atgacctgcc gttactaatc
     3601 gcgtttggtg tcatcggcat cgccgtattc tatgtggtgc aaatcatcag cgtggaatcc
     3661 gcgggcgtcg cagtgacgtg ggtaatgctg tacaccgcgc cgatctgggt agtcttattc
     3721 agcgctatct ttctgcacga acgcatcacc caacctaaaa tgatcgccat tgcgttatcg
     3781 ctggccgggt gtgcactagt ggtaaaaatt tatcaaggcg cagcgatcaa tctgtccggc
     3841 ttattagccg gtttagctac cggcctgacg tatgccgcat acattctatt cagcaaagcc
     3901 gcttcagcgc gaggttataa cacgtggacc ttcatgctct acggcttact ttttggcgcg
     3961 atctgtttat tgcccttgca atcgattgac gatctcaccc gatcgatcgc agcgcccggt
     4021 gcgattctat ggctcatcct gttgtgcctg ggtccgacct ttgccgcggc cacgatcttc
     4081 atcaccggtt taaaatacgt ccgcgccagc aatgccagca ttgtcgccac gattgagccg
     4141 gtgttggcca cactcctcgc gttgatcttc ctgcatgaat cattagagcc actccaactg
     4201 atcggagccg gcttgatctt atccgcagtg atcgtgctgc ttcgatcgcc ggatcgatcg
     4261 gagcgcgtag cgatatagcc agctgcgcaa taaaaactgc cttcctctga gcagagaaag
     4321 acagttcatg ctctccggta tggagaggac tggctgagtc gccagttaca tatcgagaac
     4381 gaggagaatt ccagcgacga tccagaggat ggcccacaca acgccgagac cagccaagaa
     4441 cggcaggaac gatgacagcc cggttagaat cgccaagatg cccaacaaca atagacccca
     4501 cttcttttgc agcttcattg cagttcctcc tgcctcgcat cgctgcgagg ttactatatg
     4561 catcgcgtca gcgcacgcga tcatgcgtct gcgcgttaca tccacggata ttagctgccc
     4621 gatcgggcag ccgggttagc cgactgagcc gcacgctttt ctatgcgacg cgcgcgcggc
     4681 ccgacgatcg gctcggtacc attcagcatg cgtttgatat taggccgcag cgcaaacgca
     4741 accaagatca gcgcgcccaa gccataaacc acgtgttccc acggaccgta gccggtagct
     4801 gcgcgaatca caaatacaac gatgatcgcc ccgctgataa tcaatgaagt caatgaggca
     4861 acgccgatca ctaataggcc gatcggcaga aatggaagcg tgaagagtag agacgggaac
     4921 caaagagcgc cggccactcc catgctagga gcagtgccgg cgccgccgcg aaagccaaga
     4981 taaatcgaag cattgtggcc gagcaagaca ccccaaccac caaagactgc cgcccacgct
     5041 gaagtcggga agatcaattg ggctaaagcg acacccgcaa agcctttcaa taaatcgcct
     5101 aacaccgtaa acgcggccgc acccataccg ctggcgcgca tggcattcgt gcctccagtt
     5161 cgtccgctgc ccgaatcgcg gatgtcacgc tcgtgaagta cagctacaat aataaaaccg
     5221 aatggaatcg agcctagcaa ataaccaccc aacgcggctg ctagccagcg accggtatct
     5281 gtccagctgg tcgccgttgc agtcaattgt cctcgaagag aattctcggt taggatgatc
     5341 gcgattacaa cgatggctgc aatcaatgcc agtaatgctc cgcctcgata aactctacgc
     5401 ttcatgcaaa ttttccgatc ggtgtgagtg ccctactgtc tgccgtctat aacaccaaaa
     5461 ccagctggtc gatactgttt cgctaattta gcgttatatc aacttcgctc actcgcctat
     5521 tcacaatttt aaagccgcgc acgcttggcg gcgaaacatc caatgagaga atgaagtaga
     5581 ttgaatctgg atagttggct tccgctatat ccgtggccga gggagtggct ggcccggaaa
     5641 tatgtgaatg ataaatgccg attggatcaa agtcacacgt ctgcatggcc tcgataaatt
     5701 ccggcccgtc catgcgatag gccgatggac tgcgtaatgc attgggcacc ggcaagactc
     5761 gattgatctc cccggcctca ccggccagca aaccgcaccg ctcttgactc ggatcagata
     5821 atacgtgcgc cagcatttga ttgacgagcg cgagcgaaaa gattacggcc tctctacccc
//
LOCUS       DASRTJ010000405         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_24938,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000405 DASRTJ010000000
VERSION     DASRTJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24938"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VFF70_08220"
     CDS             complement(<1..1353)
                     /locus_tag="VFF70_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44719.1"
                     /translation="MTRTEWRWVLIWISIALIITSLPYAIGLARSTPDKVFGGFVIAI
                     EDGNSYLAKMNEGAHGAWLFHLPYTSEPHTGTFFYLFHLLLGKVSAVTSLAPIIVYHL
                     ARLIFGALLLLTIYRFIAMFIASRAARRIAFLLIIFSGGLGWLLILLGQTGEPIDLIS
                     PEAFTFLVVYAFPHIALARTFLLLGFILVWDADPHNSARDRRAVWAGVCWLLMGIIVP
                     FYVAVVYAILVAAWLASSIGQRRIDWSEAGRSIVAGVIATPILIYTFIVVSTDPIWSN
                     WAAQLVILSPSPLQYVAAYLVVGALAVMGILHVRRSNLQPQASNFHRLIGWVIIIPFI
                     IYLPFNSQRRLIESWQIPLCIFASIGLVYRVLPTWRRSRVVQRLSQHRRYSARGLRVW
                     AWSALLFLSAATYILLLSEQSTRMLAQLPPSFRDGTEIAALDWLNQQATYDDVVLSSY
                     N"
     gene            complement(1452..>2463)
                     /locus_tag="VFF70_08225"
     CDS             complement(1452..>2463)
                     /locus_tag="VFF70_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44720.1"
                     /translation="FVALALLIPLACLLIILRLKNKLPPDRFTILIVALIVIDLISID
                     WALYRSIPIDQAFADGREVAQWIAGQSESARVYSPSYSIPQQVAQEYHLQLADGIDPL
                     QLARYVAFMQKATGVGEWKYSVTLPAFLNINSDEDIRSALANVTPNVELLGLLNVKYI
                     VSAFPIEHIDLIERARFGSTFVYENVRVMPRAFVLGQIDAVPDADAAARWLANNEVTA
                     AAVVEGLSQSIDLKIQPYEAQIVSAQPDRIEVQASGPGLLVLSEIVTPDWITTIDDQP
                     AEIFPTDLTLRGVVLPWGDHTIVFSYQPKRVYAGVLISVLSLFVCAVAISVRKFSRRL
                     SP"
BASE COUNT          703 a          670 c          610 g          480 t
ORIGIN      
        1 gttatatgaa gacaacacga catcatcata tgtagcttgt tgattcaacc aatcgagcgc
       61 cgcgatctca gtgccatcgc gaaaacttgg cggcagctgg gccagcatgc gcgttgattg
      121 ttcactcaac aagagaatgt aagtagccgc cgaaagaaac agcaaagctg accaggccca
      181 aacccgcagg ccgcgcgccg aatagcgtcg atgttgggac aagcgctgca ccacacgcga
      241 tcgacgccac gttggcagca cgcgataaac caaaccgatc gaggcgaaga tgcacagcgg
      301 aatctgccaa ctctcgatca agcggcgttg cgaattgaac ggcagataga taatgaaggg
      361 aatgatgatg acccaaccga tcaggcgatg gaaattggaa gcttgaggtt ggaggttgga
      421 tcgccgcaca tgaaggatac ccatgacggc caaggcaccc acaactaaat aggcagcgac
      481 atactgcaac ggactcggcg ataaaatcac cagctgcgcc gcccaattgc tccaaatggg
      541 atcagtgcta actacaatga aggtgtagat caaaatcggt gtagcgatca cacccgccac
      601 aatcgatcga cccgcttcgc tccaatcgat ccggcgttgg ccgatcgagc tggctagcca
      661 agccgcaact agaatcgcgt acacgaccgc aacatagaat ggaactatga tgcccatgag
      721 cagccaacac acgccggccc agaccgcgcg tcgatcgcgg gccgagttat gcggatcagc
      781 atcccacact agaatgaaac caagcagcaa gaatgttcgc gctaaggcaa tatgcgggaa
      841 agcgtacacg actaaaaacg taaacgcctc aggtgaaatc agatcgatcg gttcacctgt
      901 ttggcctagt aatatcagca gccagcccaa tccgccgctg aagatgatca gcaggaacgc
      961 aatgcgccga gccgcacgcg aagcaataaa catcgcaatg aaacgataga tcgtcaacaa
     1021 tagaagcgcg ccgaagatca atcgagcgag atgatagacg ataatcggcg cgaggctggt
     1081 cactgcagaa actttaccca gcagcaaatg aaagagataa aaaaatgtgc cggtatgcgg
     1141 ttcggaggta tagggcaagt ggaacaacca agcgccgtgt gcgccttcgt tcattttagc
     1201 cagatacgag ttgccatctt caatcgcaat cacaaagccg ccgaaaactt tatcaggcgt
     1261 cgaacgtgcc aatccgattg catacggcaa gctggtgatg atcagcgcga tgctgatcca
     1321 gatgagcacc cagcgccatt cagttcgcgt cacttcattg tgttgaatca taaattattg
     1381 aggagtcttg tctatcatgc tgagcgcagc gaagaatctc tctcctaatt agtagagact
     1441 cttcgctgca ctcagggtga caatctgcga ctaaattttc ttacgctgat cgcgaccgca
     1501 caaacgaaca aactcagcac cgagatcaat actccagcat acactcgctt cggttgatat
     1561 gaaaacacga tcgtatgatc gccccacggc agcacgacac cgcgcaaagt gagatcggtt
     1621 ggaaatattt cagcgggttg atcgtcgatc gttgtgatcc aatcgggcgt gacaatttca
     1681 ctcaagacca acaagcccgg gccggaagcc tgcacttcaa ttcgatcagg ttgcgcgcta
     1741 acgatctgcg cctcatacgg ctggattttc aaatcgatcg attgcgacag tccctcgacc
     1801 accgccgccg cagtgacttc attgttagcc aaccatcggg ctgcggcatc agcgtcaggc
     1861 acggcatcga tctgccccag cacaaatgct cgcggcatga cccgcacatt ttcataaaca
     1921 aatgttgatc caaagcgggc acgctcgatc aaatcgatat gctcgatcgg aaatgcagac
     1981 acgatatatt tcacattcaa caaacccagt aattcgacat tgggtgtcac attcgctaac
     2041 gccgatcgaa tgtcctcatc cgaattgata ttcagaaacg cgggcagtgt caccgaatat
     2101 ttccattcgc cgacgcctgt tgctttttgc atgaacgcca catagcgcgc caactgcagc
     2161 ggatcgatcc cgtccgctaa ttgtaaatga tattcctgcg ctacctgttg cggaatcgaa
     2221 tacgacggcg agtaaactct cgcggactcc gattgcccag cgatccattg agcgacctcg
     2281 cgcccgtcgg caaacgcctg atcgattgga atcgatcggt acaacgccca atcgatcgag
     2341 attaaatcga tcacgatcag ggcgacgatc aagatcgtga atcgatctgg aggcaatttg
     2401 tttttaagcc gcaagataat caagagacac gcgagcggga ttaacagcgc gagcgcaaca
     2461 aaa
//
LOCUS       DASRTJ010000406         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_327916,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000406 DASRTJ010000000
VERSION     DASRTJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327916"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="VFF70_08230"
     CDS             <1..2053
                     /locus_tag="VFF70_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44721.1"
                     /translation="YPSRSHYDERHTYNLYIVNADGSNVHRISSERGGLLHPTPLPDG
                     RILVARWWNNFNQPSDQSIYNRIDNQSRDHVLPDGTLIYSNTDETFNPALGRLALGYK
                     LRQAPNTWHLMVIDPDGTQFQRYAFTPYSEYELTNDSGNDTYVGAQPAVVLSGTQQFI
                     AFTSQQDESMVHSTLRTGIRVARPDISMMYSNTADAIAGLTYDKVWGHGDTSPPYAIH
                     PWGLPDGSILFSYAAGPIGGLPSIGSYNDPVTGRNFDLQGSALQYQLYTMQIDGSNKT
                     LITTTIGTADAMDAKPIVARLGWATISDTFTSNANDDPRHGNVPNNLPQYSFSLSDTH
                     QILTATLHNPNVYANAPLTLPFINNSPMPGSVAFAQVYIDANQFTGAYCYGPGYPDPC
                     TTFKSDSEVRAVLWTQVPVSLKGEFTATVPADTPGFVVLRDANGRAVSGWNRGYVSIA
                     QGSAWARPAETVTCVGCHLGHVSGSIDQVLTQSVQGWTNVAPYANVTASSYFTNGNST
                     YQPFVPSKINDRQGWVPIPIGGSGGTYQDDETGWISKDKSAVGEGITLTWPSAMTITK
                     VRLVGPPPIDGDWGGFGHYAITSPYHIVSSTLQFYLGNGLVGGPIAVGQVEPLSNSGT
                     LINLPQPIQIDRLRFTVNAINGKWWWDDVAALNEIEVIGMAAERYTINIQRVYLPIIR
                     K"
     gene            complement(2112..2477)
                     /locus_tag="VFF70_08235"
     CDS             complement(2112..2477)
                     /locus_tag="VFF70_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HZY44722.1"
                     /translation="MKCPKCSSAMEKVIYEGIEVDRCTNCQGIWFDFQEQKQLKDKKG
                     ADSIDIGEATHDKPVSKMYCPRDGLLLTQMVDVRQPHIWYEACPHCYGVFFDAGEFRD
                     YSHKSVIESVKDLFTHERK"
     gene            complement(2499..>2897)
                     /locus_tag="VFF70_08240"
     CDS             complement(2499..>2897)
                     /locus_tag="VFF70_08240"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HZY44723.1"
                     /translation="MEFSPDESTLIEENIETLHTIGLHLDAFGGSTWLIRSIPSLLAK
                     DDIRTAMNELVADLMAGDLPFAANEEAKLITRVCKRAAIKGGQVLSTQEMNELIRQLE
                     VCASPRTCPHGRPTMIHFSASQMAKEFGRI"
BASE COUNT          657 a          772 c          750 g          718 t
ORIGIN      
        1 ttatcccagt cgatcgcatt atgatgagcg gcatacttac aacttataca tcgtgaatgc
       61 tgacggatcg aacgtgcatc gcatcagcag cgagcgcggc ggtttgctgc atccgacgcc
      121 gctgcctgat ggaagaattc ttgtcgcgcg gtggtggaat aacttcaatc agcccagcga
      181 tcaaagcatc tataatcgca ttgataatca atctcgcgat cacgttttac cggacggcac
      241 gttgatctat tccaacaccg atgaaacttt caatcctgcc ttaggtcggc tggcgctcgg
      301 ttataaattg cgccaggcgc cgaacacctg gcacctcatg gtgatcgatc cggatggcac
      361 gcagttccag cgttatgcct tcacgccgta cagcgaatac gaactcacca atgattcagg
      421 caatgacact tacgtcggcg cgcagcccgc ggtagtttta tcgggcacgc aacaattcat
      481 cgccttcacc tcgcagcaag atgaaagcat ggtgcattca accttgcgca ccggcattcg
      541 cgtcgcgcgg cctgacatct cgatgatgta ttccaacaca gcggatgcga ttgcgggctt
      601 gacgtacgat aaagtctggg gtcatggcga tacttctccg ccatatgcga tccatccgtg
      661 gggtctgccc gatggatcga tcttgttttc gtacgccgct ggtccgatcg gggggctgcc
      721 ctcgatcggt tcttacaacg atccggtgac cggacgaaat ttcgatctgc aaggatcagc
      781 tcttcaatat caactgtata ccatgcagat tgatggatcg aacaagacgc tgatcacgac
      841 gacgatcggc acagccgatg caatggacgc gaaaccgatc gtcgcccgat tgggctgggc
      901 aacgatctcg gatacgttta catcgaacgc caatgacgat ccgcgtcacg gcaatgttcc
      961 aaataatttg ccacaatact catttagttt aagtgacaca catcaaattt tgacggcgac
     1021 gctgcacaat cctaacgtgt atgccaacgc cccactgact ttgccgttca ttaataattc
     1081 accgatgccc ggcagcgtcg ccttcgcgca agtgtacatc gacgccaatc aattcacggg
     1141 cgcatactgt tacggtccgg gttatcctga tccttgtaca acgtttaagt ccgatagtga
     1201 agtacgtgcc gtgctatgga cacaagtgcc cgtgagtttg aaaggagagt tcactgcgac
     1261 tgttcctgcc gacacacccg gctttgttgt cctgcgcgat gcgaatggcc gcgcggtcag
     1321 tggttggaat cgcggctatg tttcgatcgc acagggcagc gcgtgggcgc ggcccgctga
     1381 aaccgtaacc tgcgtcggtt gtcatctcgg tcacgtcagt ggttcgatcg atcaagtgct
     1441 gacgcaatca gtgcaaggct ggacaaatgt cgcgccgtat gcgaatgtca ccgcgagcag
     1501 ctatttcacc aatggcaatt cgacttacca accgttcgtg ccgtcgaaga tcaatgatcg
     1561 acaaggctgg gtgcccatcc cgatcggtgg ttcgggcgga acgtatcaag atgacgaaac
     1621 aggttggatt tcgaaggaca aatcggcagt aggcgaaggc atcacgctga cttggccgag
     1681 tgccatgacg attaccaaag ttcgtttagt cggtccgccg ccgatcgatg gtgattgggg
     1741 tggttttggg cattatgcga tcaccagtcc atatcacatt gtctcaagca ctctgcaatt
     1801 ttatttgggt aatggtttgg ttggtgggcc gatcgcggtg ggtcaggtcg agccgttatc
     1861 aaatagtggc acgttgatta acttgccgca gcccattcag atcgatcgat tgcgcttcac
     1921 agtcaatgcg atcaacggca aatggtggtg ggatgatgtg gcggcgttga atgagataga
     1981 agtgatcggc atggcggcgg aacgatacac gatcaatatt caacgcgtgt atctaccgat
     2041 cattcggaaa taaataattg tagcggcagg cggctgcctg ccctgggcga acagccgctc
     2101 gcccctgcgc gttattttcg ttcgtgagtg aataaatctt tgacggattc gatcacggac
     2161 ttgtggctgt aatctctgaa ttcgcccgca tcgaagaaca caccatagca atgtggacag
     2221 gcttcgtacc aaatatgcgg ttgtctcaca tcgaccatct gagtcaacag taagccgtcg
     2281 cgcgggcaat acattttgct gaccggttta tcgtgagttg cttcgccaat atcgatcgag
     2341 tctgcgcctt tcttgtcctt cagttgcttc tgctcttgaa aatcgaacca aatgccttgg
     2401 cagtttgtgc agcgatcgac ctcgatgcct tcgtaaatta ccttctccat tgctgaactg
     2461 cactttggac acttcatggt tgctcctcgg gcttgaaatt agattcggcc aaactctttc
     2521 gccatctgtg atgcgctgaa atggatcatt gttggtcgac cgtgcggaca cgttcgcggc
     2581 gacgcgcaca cctcgagttg gcgaatcagt tcgttcatct cctgcgttga taatacttga
     2641 ccacctttga tcgccgcgcg cttgcacacc cgcgtgatca actttgcttc ttcgttggcc
     2701 gcgaagggca gatcgcccgc cattaaatct gcgacgagtt cattcatcgc cgttcgaatg
     2761 tcatccttcg ccaacaacga tggaatactg cgaatcaacc aggtcgatcc gccgaatgca
     2821 tcgaggtgca atccaatcgt atgcaacgtc tcgatgttct cttcgatcaa tgtcgattca
     2881 tcaggcgaaa attccat
//
LOCUS       DASRTJ010000407         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_124128,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000407 DASRTJ010000000
VERSION     DASRTJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124128"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..927
                     /locus_tag="VFF70_08245"
     CDS             64..927
                     /locus_tag="VFF70_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZY44724.1"
                     /translation="MKRLHAFLDLIKFEHTIFALPFAYLGMILAANGLPTLTQFIWIT
                     IAMASARTLAMSFNRFADRRIDARNPRTAIRPIQTGKISVRTVVIGAAISLIVLSFAA
                     WQLNPLTLMLLPGAIMFLLGYAYAKRFTILSHFILGFTDGLAPIGAWAGVRGSIFTSR
                     DLPAWLLLFAVTFWIGGFDLMYACQDTEFDRAEKLHSIPARWGNAAALRWAKISHMIT
                     IGLLSAIGISMQLSWVYWIGLIIVIGLLIYEHSLVRPNDLSKINVAFFNVNGYISITI
                     FVATLIALFVK"
     gene            1035..1994
                     /locus_tag="VFF70_08250"
     CDS             1035..1994
                     /locus_tag="VFF70_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZY44725.1"
                     /translation="MFRYSLVIAVLFSVLLGACAPAAAPGAVEEPALKIGVLPILDAL
                     PMYVAESQGYFKDAGINVTFVPVASAAERDQLMQAGQIDGMINDLVSTVLYNKDTQKI
                     TIVRFARTATPEAAQYSILAAKDSGITQWSDLKDVEIGISEATVIAYATDRLLEAEGL
                     QAADIKTTNIPKIPDRLTLLASGQLKAATLPEPTASLAVQQGAVVIVDDHTHTEIGNS
                     VISFSVATLSAKPNTVKKFLAALEKAVADVNASPDKFSTLLSDKKLVPAPLIGLYKLP
                     KFPSASVPSEAQFNDVEAWMKGKNLITADVPYAKLVDASYLPK"
BASE COUNT          483 a          580 c          637 g          538 t
ORIGIN      
        1 gaatgaaata acgagtaaca agtaccgagt aatttactcg ttgcttgtta ctcgttactt
       61 cgcatgaaac gacttcacgc cttccttgat ctgattaaat ttgagcacac catcttcgca
      121 ctgccgttcg cttacttggg aatgatcctc gccgcgaatg gattgccgac gctgacgcag
      181 ttcatttgga tcacgatcgc aatggcctct gctcgaacgc tggcgatgag cttcaatcgc
      241 ttcgccgatc ggcgtatcga cgcccgcaat ccccgcactg cgatccggcc gatccagaca
      301 ggcaagatca gcgtgcgcac ggtcgtgatc ggcgcggcga tatcgttgat cgtgttgtcg
      361 ttcgcggcgt ggcagttgaa cccattgacg ttgatgctgc tgccaggcgc gattatgttc
      421 ttgctcggtt atgcctacgc caaacgcttt acgatcttgt cgcatttcat tttaggtttt
      481 acggacggcc tcgcgccgat cggagcgtgg gcgggagtac gtggctcgat tttcacatcg
      541 cgcgatctgc ccgcatggct gttactgttt gcggtcacat tctggatcgg tggcttcgat
      601 ctgatgtacg cctgtcagga tacggagttc gatcgagcgg agaagctgca cagcattccg
      661 gctcgctggg gtaatgcagc tgcgctgcgt tgggcgaaga tcagccacat gatcacgatc
      721 gggctgctga gcgcgatcgg gatcagcatg caactcagct gggtgtattg gatcggcttg
      781 atcattgtga tcggtttatt gatctatgaa cattcactcg tcaggccaaa tgatctatcg
      841 aagatcaatg tggcgttctt caatgtgaac ggctatatca gcatcaccat ttttgttgcg
      901 acgttgatcg cgttattcgt caaatgaagt caaacagcag gcggcgatac gcgcgccaca
      961 cctgcacttg cgcgctaccg cgcgcagcgc ggtgcaagtg tcagccttct gcttcaagcc
     1021 aatcaaggag aaccatgttt cgctatagtt tggtcattgc agttttgttt agtgtgttgt
     1081 tgggcgcgtg cgcgcccgcg gcggcgcctg gcgcagtcga agagcctgcg ttgaagatcg
     1141 gcgtgttgcc gatcctcgat gcgctgccga tgtatgtggc cgagtcacag ggatatttca
     1201 aagacgcagg catcaatgtg acgttcgtgc cggttgcttc ggcggctgaa cgcgatcagt
     1261 tgatgcaggc cggtcagatc gacggcatga tcaacgatct ggtgtccacc gtcttataca
     1321 acaaagatac gcagaagatc acgatcgttc gatttgcccg aacggccacg ccggaagccg
     1381 cgcagtattc gatcctcgcg gcgaaggaca gcggcatcac ccagtggagc gatctcaagg
     1441 acgtcgagat cggcatcagt gaagcgactg tgatcgctta tgcgaccgat cggctcttag
     1501 aggctgaagg cctgcaagcc gccgacatca agacgacgaa catccccaag attccggatc
     1561 gattgacgct gctcgcgtcg ggacaattga aggccgccac gttgcccgaa ccaacagctt
     1621 cattagcagt acaacaaggt gcggtcgtca tcgtcgatga tcacactcac acggagatcg
     1681 gcaacagcgt gatctcattc agcgtggcaa ccttgagcgc gaagccgaat actgtcaaga
     1741 agttcttggc cgcgcttgag aaagcggtcg cggatgtgaa tgcctcgccg gataagttta
     1801 gcactttact gagtgataag aagttggtgc ctgcgccgtt gatcggattg tataagctgc
     1861 ctaagttccc gtccgcctct gtgccgagcg aggcacagtt caacgatgta gaagcgtgga
     1921 tgaagggtaa gaatttgatc acggctgatg tgccgtatgc taagttggtc gatgcgagtt
     1981 atttgccgaa gtaacctcac tccctcctat cctgccgatg caatgaaagt tgattaagca
     2041 ttgatccaac gtatcgtcag gataggggac aaagaggtga ggaagaggac gggagatcaa
     2101 gtataatgcc tctcgtcctc ttttcgatct gacatgatca attttcacga agtcaccttt
     2161 gcctatccca acggccgccc cgtattcgat cggttcagcg gctcgatcga gcggggtgaa
     2221 gcgtgggccg ttgtcggc
//
LOCUS       DASRTJ010000408         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_252341,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000408 DASRTJ010000000
VERSION     DASRTJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252341"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFF70_08255"
     CDS             <1..604
                     /locus_tag="VFF70_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44726.1"
                     /translation="FLGFSVLTIASAFVVKILHGNSETYGLLLSASGAGALIGVLFVV
                     PIAQAAHRTGLILIATSAWMGTWFIVLASTTVIAVANLSIFMFSIGAPTVITMALGLI
                     QVLAPPNMRARLVTVFIMITFGMQPVASVLVGLSAETLSIPTAILINGVLFVIGAAAM
                     FLFRPELRHWSANQHSAAALQVAKPPTADAVSEISKGLEI"
     gene            711..1562
                     /locus_tag="VFF70_08260"
     CDS             711..1562
                     /locus_tag="VFF70_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY44727.1"
                     /translation="MYESIHPNTHLGHVSITIPNMDRSLDFYQNALGMKVHRKGDGTA
                     MLGGGGADVLELIEDPSAKRASRTSGLYHFAILRPSRFELAQSLRRLAEARWRSGSAD
                     HLVSEAIYLSDPDGNGIEIYRDRSRSDWPRFNGQIQMATDPLDVEGILAELEGHAEPW
                     TGMEDKTILGHMHLHVGDVNKADHFYNGVLGFDNIAMLGDSAGFVSAGGYHHHIAFNT
                     WNGVGAPPQPPNSIGLRNFLVRLPDSTELGKIADRVRLAGLDIEEENEGLLVRDPSQN
                     AVVFTVD"
     gene            1575..1913
                     /locus_tag="VFF70_08265"
     CDS             1575..1913
                     /locus_tag="VFF70_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011181661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HZY44728.1"
                     /translation="MSVPGAGEQIKQAVLSWPDITAEPHRFGGTEFRIGTRELGHIHG
                     DALVDIPFPTKVRNELVTGGRAQPHHILPESGWISFNLRTVEDIQHAIDLLRLSFELA
                     LKQKSRGQAE"
     gene            complement(2003..2344)
                     /locus_tag="VFF70_08270"
     CDS             complement(2003..2344)
                     /locus_tag="VFF70_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44729.1"
                     /translation="MYKWQCPNCGWAGQITDELQAAAVREADKIKATHHIEHCPRCQW
                     VVRIPVDVLRTAALTAKPAAQKSIAKKSAAKKKAVKKVAAKKPAKKPAVKKAVANKRP
                     AAKKPASKRRQ"
     gene            complement(2473..2790)
                     /locus_tag="VFF70_08275"
     CDS             complement(2473..2790)
                     /locus_tag="VFF70_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44730.1"
                     /translation="MSRDLQQDPNTMPKTTWPAPDPVTPMLPATTPTPTPWTPPAMPM
                     AEPMMPAKKKAAPKKKVVKKPVKKLVKKAAPKKKAAKKPAKKVKKAAPKKKAAKKSKK
                     SKK"
     gene            complement(2867..3331)
                     /locus_tag="VFF70_08280"
     CDS             complement(2867..3331)
                     /locus_tag="VFF70_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44731.1"
                     /translation="MMQIRCQRCGWNFTLGRDTIGLAVAESNAGHQEYTQFICPNCRH
                     EVKVQVEVLRKHLPMDYVLPVLQAKTEPIAIPKEVSKPTTATVAKPAAKPAPKPVKPA
                     GSIEPQQAASSTAKKQPGTKKIGGKKPIVKKPGAAKPASKKSAASTAKKSKK"
     gene            complement(3388..4338)
                     /locus_tag="VFF70_08285"
     CDS             complement(3388..4338)
                     /locus_tag="VFF70_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY44732.1"
                     /translation="MPHRSQLRLISILFIGFLAVSTSSILIRLARAEGVPALVIAAYR
                     LTIATLILTPIVLRRHRAELKRLTRRDALIAVASGLFLTLHFATWISSFDYTSVTSSV
                     VLVTTGPIWVALASWIILRERLSHPMIVGVIITVVGGVVVGLADACDLKTGSCSGLSI
                     GGTQLWGDALALIGAWMVTGYLLIGRYLRKRMPLLIYIFLVYGTAAIGLILIVALSGQ
                     SFVFDQFGEIFSLPAVVWLILLAIAPQLLGHSAYNYALRFLSPAFVAIITVAEPIGAS
                     ILAIVILHELPGPLTLIGAVIILIGIAVASQPAPANFPAT"
     gene            4507..>4787
                     /locus_tag="VFF70_08290"
     CDS             4507..>4787
                     /locus_tag="VFF70_08290"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZY44733.1"
                     /translation="MAFELPALPYDYSVLEPHIDEQTMRIHHDKHHAAYVTNLNKALE
                     GAAEWHSKSIEDILKNLNSVPENIRTAVRNNGGGHYNHSMFWQIMGPNMG"
BASE COUNT         1109 a         1279 c         1257 g         1142 t
ORIGIN      
        1 tttcctgggc ttctcagtct tgaccatcgc ctctgctttc gtcgtgaaga tattgcatgg
       61 caattctgaa acttacggtt tattgttatc ggcctcgggt gcgggcgcat tgatcggtgt
      121 gctgttcgtt gtgcccatcg cccaagcggc gcaccgcacc ggtctcatcc tgatcgcgac
      181 cagtgcgtgg atggggacct ggttcattgt cttggccagc accactgtca ttgcagtggc
      241 caacctgagc atcttcatgt tcagcatcgg cgctccgacg gtcattacca tggcgctggg
      301 tttgattcaa gtgctggccc cgccaaacat gcgcgcacgt ttagtcacgg ttttcatcat
      361 gatcactttt ggcatgcagc ccgtcgcctc tgttctcgtc ggcctcagcg ccgagacgtt
      421 atcgataccg acggcgatct tgatcaatgg cgtcttgttc gtaatcggcg cggcggcgat
      481 gttcctcttc cggcccgagc tgcggcactg gagcgcgaat caacattcag ctgcagcact
      541 gcaagtcgct aaaccaccaa ccgccgatgc cgtttcggag atttctaaag gtcttgagat
      601 ttagcgactt attgaagacc aattatatgt tatccaaatt tcacatcctt gtaagtgatc
      661 ccaaagccga ttgctgagcg caatctattg atactaatga ggttactgat atgtacgaat
      721 cgatacatcc caatactcac ttaggccacg tctcgatcac gatcccaaat atggatcgat
      781 cgctggactt ctatcaaaat gctcttggca tgaaggtaca tcgaaagggt gatggcacgg
      841 caatgctcgg cgggggcggc gcggatgtgc tggaactgat cgaagatcca tctgccaagc
      901 gcgcatctcg caccagcggc ttgtatcact ttgcgattct gcgcccatcg cgttttgagt
      961 tggcgcaatc gctgcgacgc ttagctgaag cgcgctggcg ctcaggctct gccgatcatt
     1021 tagtgagtga agcgatttat ctgtccgatc cagatggcaa tggcatcgag atatatcgcg
     1081 atcgatctcg atcggactgg ccgcgcttca acggacaaat acaaatggcg accgatccac
     1141 tcgatgttga aggcatcctg gccgaactcg aagggcatgc agagccgtgg actggcatgg
     1201 aagataaaac gatcttgggc catatgcatt tgcatgtcgg cgatgtgaac aaagccgatc
     1261 atttttacaa cggtgtgctc ggctttgata acatcgcgat gctgggcgac tcggccggct
     1321 ttgtatcggc gggcggttat catcatcaca ttgccttcaa tacctggaac ggtgtgggtg
     1381 cgccgccgca gccaccaaac tcgatcggct tgcggaattt cctcgtgcgg ctgccggact
     1441 cgaccgagct gggcaagatc gccgatcggg tacggttggc cggtctcgat atcgaagaag
     1501 aaaatgaagg tctgctcgtg cgcgatccat cgcagaatgc agtggtgttt acggtggatt
     1561 gatctggagc gaaaatgtcc gtacctggtg caggcgaaca aatcaaacaa gcggtattga
     1621 gttggccgga tataaccgca gagccgcatc gctttggcgg cacggagttt cgcatcggca
     1681 cgcgcgagct cggtcacatt cacggcgatg cgctggtcga tattccgttc ccgacgaaag
     1741 ttcgaaacga actagtcacc ggaggtcgag cgcagccgca tcacattttg ccggagagcg
     1801 gctggatcag tttcaatcta cgtacagtag aagacatcca acacgcgatc gatctgctcc
     1861 ggttgtcgtt cgagttggcg ctgaaacaaa aatcgcgcgg ccaggcagaa taggaaagtt
     1921 aatcgatcac tgcgcttcat aagaagagcc ccacactttg atcgagtgtg aggctctttc
     1981 attgactttt gccacttcaa agttactgcc tgcgtttaga cgccggcttt ttcgcggctg
     2041 gtctcttgtt cgcaaccgct ttcttgacgg cgggcttctt agccggtttc ttggccgcaa
     2101 ctttcttgac ggcttttttc ttcgcggccg atttcttagc aatggatttc tgggcagccg
     2161 gtttagctgt cagcgccgca gtgcgcagca catcgaccgg aatgcggacc acccattggc
     2221 agcgcggaca atgttcgata tgatgcgtcg ccttaatttt atctgcttcc ctaaccgctg
     2281 cagcctgaag ctcatccgta atctgtccgg cccagccgca gttgggacat tgccacttgt
     2341 acatactcac tcctccttga aacgccaatc gctgaataac tgtattgtac acacgcgaag
     2401 tcgaaatgca aatcaacaat aaaggccgca cgcaactcgt ggggccttta tctaaaatga
     2461 tcaaatctgg cgttatttct tggatttctt agatttcttg gccgctttct tcttgggggc
     2521 ggccttcttt actttcttag caggcttttt agctgccttc ttcttggggg ccgccttctt
     2581 gaccagtttc ttcacgggtt tcttgaccac tttcttcttg ggggcggctt ttttcttcgc
     2641 cggcatcatc ggctcagcca tgggcattgc cggtggcgtc cacggagttg gcgtcggcgt
     2701 cgtcgctggc aacatgggtg ttaccggatc tggcgccggc caggtggtct tgggcatcgt
     2761 attcgggtct tgctgcaaat cgcgtgacat tgttgagcct cctgagtttt tgatttagat
     2821 tgatcacgac acgtgtgaaa cacgtgttat tacctattaa atgctgctat ttcttggatt
     2881 tcttggcagt gctagccgct gacttcttag acgctggttt tgctgctcca ggtttcttga
     2941 cgatcggttt cttaccaccg atcttcttgg tccccggttg ttttttcgcc gttgatgacg
     3001 cagcctgctg tggctctatc gaaccagctg gcttcacagg ctttggcgca ggtttggccg
     3061 cgggtttagc cacggtggcc gtcgtaggtt tagacacttc tttaggaatg gcgatcggtt
     3121 cagtttttgc ctgtaggacg ggtaagacat aatccatcgg caaatgcttg cgcaaaacct
     3181 cgacttgaac cttaacttca tggcgacagt tagggcaaat gaactgggta tattcctgat
     3241 gccctgcatt ggattcagcc acagctaacc cgatcgtatc tcgaccgagc gtgaaattcc
     3301 aaccgcagcg ttgacatcga atttgcatca tttctaagcg gcctctccca actgacacga
     3361 ttattgtata acagacacac gcaataatca agtggcaggg aagtttgcag gggcaggctg
     3421 cgaagcaaca gcaatcccga tcaaaatgat caccgcgccg atcagcgtaa gcggtccagg
     3481 cagttcatgc aagatcacga tcgctaaaat cgatgcaccg atcggctcag cgacggtaat
     3541 aatggcgaca aaggcgggcg acagaaagcg cagcgcatag ttatacgcgg aatggccgag
     3601 cagctgcggt gcgatggcga gtaagatcag ccacacaacc gctggcagtg aaaaaatttc
     3661 gccgaattga tcgaacacaa acgattggcc gctgagcgcg acgatcaaaa tcaagccaat
     3721 cgccgctgtc ccatacacca aaaaaatata aatcagcagc ggcatgcgct ttcgcaagta
     3781 gcggccaatc aacaagtagc ccgtcaccat ccacgcgccg atcaacgcca gcgcatcgcc
     3841 ccataactgc gtgccaccaa tcgataagcc gctgcacgat cctgttttaa gatcgcacgc
     3901 atcggctaat cccacgacaa cgccacccac caccgtgata atcacaccga cgatcatagg
     3961 atgagacaag cgctcacgca aaatgatcca tgaggccagc gccacccaga tgggaccggt
     4021 cgtcactaac accacggagg aggtcacgga tgtatagtcg aaggatgaaa tccaagttgc
     4081 aaagtgcagc gtcaaaaaaa gccctgacgc aacggcgatc aacgcatctc tgcgcgtgag
     4141 gcgtttcaac tcagcacgat gacggcgtaa aacgatcggg gtcagaatca acgtcgcaat
     4201 ggttaatcga taagcagcga tgaccagcgc aggcacacct tccgcgcgcg ctaaacggat
     4261 aaggatcgac gaagtggaga cagccaggaa accgatgaag aggatgctga tcaatcggag
     4321 ttgagatcgg tggggcatat ggatcgatcg actgccagcg actgccgcag atttagcggc
     4381 aaaactgcgc gccgatcggt ttactcctct tgacaagcat tttgaataag gctacaatcg
     4441 ataaagataa atcgctgggt gattcagaat cgatttgaat cataccaagc aacaggagag
     4501 ttgacaatgg cctttgaact tcccgccctg ccctatgatt acagtgtcct cgaaccgcac
     4561 atcgatgagc aaacgatgcg gattcatcac gacaagcatc acgccgcata tgtcaccaac
     4621 ctcaacaaag ccctcgaagg cgccgccgag tggcacagca aatcgattga agacattttg
     4681 aagaacttga atagtgtgcc ggaaaatatc cgcaccgccg tgcgcaacaa cggcggcggc
     4741 cactataatc actccatgtt ctggcagatc atggggccga atatggg
//
LOCUS       DASRTJ010000409         9092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_328391,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000409 DASRTJ010000000
VERSION     DASRTJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328391"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..259)
                     /gene="rpsQ"
                     /locus_tag="VFF70_08295"
     CDS             complement(5..259)
                     /gene="rpsQ"
                     /locus_tag="VFF70_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZY44734.1"
                     /translation="MNKQRKRLVGRVTSDKMQKTVVVTVDQLKRHRLYSKVIRRSHKY
                     KAHNPADVAKTCDLVQIVEAQPLSRVKRWAVESILEKAQA"
     gene            complement(256..471)
                     /gene="rpmC"
                     /locus_tag="VFF70_08300"
     CDS             complement(256..471)
                     /gene="rpmC"
                     /locus_tag="VFF70_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZY44735.1"
                     /translation="MTVTELRALNTQEIQHKLAASHQELFNLRFQWASGRLEDHNRIT
                     MLRRDVARLQTVLRERELAALAQGESK"
     gene            complement(476..907)
                     /gene="rplP"
                     /locus_tag="VFF70_08305"
     CDS             complement(476..907)
                     /gene="rplP"
                     /locus_tag="VFF70_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZY44736.1"
                     /translation="MLMPKRVKWRKQQRGRMKGKELRGVEVVFGDLGLQALEPCWMTQ
                     RQIEAARRAVVHFVKRRGKMWVRIFPDKPVTQKAAETRMGKGKGAVDHYVAVIKPGRI
                     ILEIGGIDDATAREALRLAAYKLSIKTQIIGRRDLASMSGE"
     gene            complement(926..1603)
                     /gene="rpsC"
                     /locus_tag="VFF70_08310"
     CDS             complement(926..1603)
                     /gene="rpsC"
                     /locus_tag="VFF70_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZY44737.1"
                     /translation="MGRKVHPYGFRLGVIRDWNARWFAKKGKQYAQFLVEDYRLRNYI
                     RKETAQAGVSKIAIERFPNQIIVTVHTAKPGVIIGRKGAMVKQLRESLEANTGKKVKL
                     EIAEIDKPDLEASLVADNIANQLERRISHSRAMKRAIQQAMRVGAEGIKITCAGRLAG
                     SEMARRETVMEGRVPRSTLRADIDFARSEALTTYGRIGVKVWIYKGEILNEAKEQRTA
                     RIAPVEE"
     gene            complement(1634..1987)
                     /gene="rplV"
                     /locus_tag="VFF70_08315"
     CDS             complement(1634..1987)
                     /gene="rplV"
                     /locus_tag="VFF70_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZY44738.1"
                     /translation="MAVQTDAHAVARFVQVSPRKVRLVINQVRGLQADKALAVLKFLP
                     NHAAEPILKLIVSAVANAEENLGLSRTDLYISEIKADEGPRRQWRRFGARGRFKPILR
                     RSSHITVVLSERVST"
     gene            complement(1999..2310)
                     /gene="rpsS"
                     /locus_tag="VFF70_08320"
     CDS             complement(1999..2310)
                     /gene="rpsS"
                     /locus_tag="VFF70_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZY44739.1"
                     /translation="MSRSLKKGPFVSAKLLKKVEAMNQTGDKKVIRTWSRASTIFPQM
                     VGHTLAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHTIREKEEAATTKVEAAPAA
                     K"
     gene            complement(2328..3161)
                     /gene="rplB"
                     /locus_tag="VFF70_08325"
     CDS             complement(2328..3161)
                     /gene="rplB"
                     /locus_tag="VFF70_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZY44740.1"
                     /translation="MPIKKYKPTSPGRRGMTGDSFEEITKSTPERSLLSDKRKHGGRN
                     STGRITVRHQGGGHKQTYRLVDFMRDKHNIPAKVATIEYDPNRTARIALLTYADGEKR
                     YIIAPVELHVGDSVMSGSKAEVRPGNCLPLANMPLGTMVHNVELLPGKGAQMARAAGV
                     SAQLLAKEGDYATLRLPSGEMRKVRQECFAVVGQVSNVDHGNVKLGKAGRSRHRGIRP
                     TVRGSAMSPRDHPHGGGEGRTGVGMPGPKSPWGKPTLGYKTRRNKVSNSFIVRRRGKK
                     R"
     gene            complement(3214..3531)
                     /gene="rplW"
                     /locus_tag="VFF70_08330"
     CDS             complement(3214..3531)
                     /gene="rplW"
                     /locus_tag="VFF70_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZY44741.1"
                     /translation="MELHVYEVLKRPLQTEKTMYLKELHQYAFEVDLRANKEAVKQAV
                     EKIFKVNVVAVHILNQPSKRRRNPRSRATGKKAQQRIRIAGWKKAIVTVQADQQIELF
                     EGV"
     gene            complement(3542..4162)
                     /gene="rplD"
                     /locus_tag="VFF70_08335"
     CDS             complement(3542..4162)
                     /gene="rplD"
                     /locus_tag="VFF70_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZY44742.1"
                     /translation="MKIPMKNMAGENIGEVELNSVIFEVPVNVPLMHQALTRQLANAR
                     QGNAKTKTRSEVAGSTRKMWKQKGTGRARQGSRKAPQWRKGGIVFGPIPRKYTQQMPL
                     KMRHAALRSALSVKAADQHIVVLDKLEFDSAKTKEMSSVLNNIEAGRSTLVVLPEHND
                     NVERSVRNLAGVKYLRAQYLNIRDLLGCDTLILPRGALDVIESMLG"
     gene            complement(4202..4876)
                     /gene="rplC"
                     /locus_tag="VFF70_08340"
     CDS             complement(4202..4876)
                     /gene="rplC"
                     /locus_tag="VFF70_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZY44743.1"
                     /translation="MKGLLGRKLGMTQIFNEGGESLLVTVIEAGPCYVTQVRTTAKEG
                     YSAIQIGFDAVKKMTKLSRGEQGHLGLVKSNAKHPKRKELAAEVPAVKHLRELRAKSD
                     EGYTEGQKLTVDLFKMGERVDVIGTSKGRGFAGVVKRHHFRGGPRTHGQSDRLRTSGS
                     IGAGTSPGKIHKGKRMAGHMGVERVTSQNLQVVLLDADRNLLGVRGAVPGATGGLVLV
                     RETRKK"
     gene            complement(4980..5288)
                     /gene="rpsJ"
                     /locus_tag="VFF70_08345"
     CDS             complement(4980..5288)
                     /gene="rpsJ"
                     /locus_tag="VFF70_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZY44744.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAQRIVETAERTGATVVGPVPLPT
                     RLERFTVQRASFIDKNSQEHFEIRTHKRLIDVLDPDSKTIDTLMRLNLPAGVDIEINL
                     "
     gene            complement(5363..6565)
                     /gene="tuf"
                     /locus_tag="VFF70_08350"
     CDS             complement(5363..6565)
                     /gene="tuf"
                     /locus_tag="VFF70_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZY44745.1"
                     /translation="MSKQHYDRSKPHVNVGTMGHIDHGKTTLTAAITKVLGLKKLATF
                     RSFDSIDNAPEEKERGITINIAHVEYETEKRHYAHVDMPGHRDYIKNMITGAAQVDGA
                     ILVVAAPDGPMPQTREHVLLARQVEVPAIVVFLNKVDMMDDPELLELVELELRELLNG
                     YGFPGDETPIVRGSALAALNEPSTDPNAPAYKPIWDLMRVVDEYIPTPKRETDKPFMM
                     SVEDVFSIKGRGTVVTGRVDRGVIKPGDPISIVGLRDEVRDTVVTSMEMFHKTLDDAE
                     AGDNAGLLLRGIERTDVERGMVLAKPGSIKPHRKFDAEVYVLKKEEGGRHKAFVSGYR
                     PQFYIRTLDVTGTITLPAGVEMVMPGDNIKMSVELITPVALEAGGHFAIREGGLTVGA
                     GVITKVAD"
     gene            complement(6588..8687)
                     /gene="fusA"
                     /locus_tag="VFF70_08355"
     CDS             complement(6588..8687)
                     /gene="fusA"
                     /locus_tag="VFF70_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZY44746.1"
                     /translation="MTREFPLELMRNIGIIAHIDAGKTTTTERVLFYTGKTHRIGSVD
                     DGTTVTDWMEQERERGITITAAAITCEWREYQINIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDGVQGVEPQSETVWRQADRYKVPRICFINKLDRTGADFYRAVASIKDRLGANPL
                     PLQLPIGHESDFRAVVDLMEMNAIYWTDDLGLKMDTRDIPADMQAEAKAAHETLLERI
                     AETDDDLTHRFLEGETLSVDELKKALRKATLAGKIVPVFCGSSLKNKGVQPMLDAVID
                     YLPAPIDVPSVTGLDPDTGESVVRTTDEGQPFCALVFKIVADPFVGRLAYFRVYSGKV
                     DVGETVLNSSKRRKERLGRVLRMYADRRVEQTVVYAGDIGATLALKDSFTGDTLCDID
                     KPVILESISFPDPVIEIAIEPKTAADQDKMGEALRRLAEEDPTFRVRTDENTAQTLIA
                     GMGELHLEILVDRMMREFKVQANVGRPKVAYRETITRPVKIEGRFIRQSGGRGQYGHV
                     WLQLEPLEKGKGFEFENGIIGGTVPREYVPAVEKGIKEAMESGVVAGYPMVDIKATLI
                     DGSYHEVDSNEMAFKIAASMALKDGAQKARPVLLEPIMKVEVVVPDEYTGDVIGNLSA
                     RRGQIEGMEPRPGNAQAIKSHVPLGNMFGYATDLRSSTQGRGTFTMEFEYYAEVSQSV
                     AEEILKNGKREPTRAMR"
     gene            complement(8798..>9092)
                     /gene="rpsG"
                     /locus_tag="VFF70_08360"
     CDS             complement(8798..>9092)
                     /gene="rpsG"
                     /locus_tag="VFF70_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZY44747.1"
                     /translation="EVFEQAMKNATPQIEVKPRRVGGATYQVPVEVPNDRRNTLAMRW
                     LLAAATSRGGKSMMEKLAGELIDAAQGNGAAIKKKDETHKMAEANRAFAHFRW"
BASE COUNT         1886 a         2718 c         2269 g         2219 t
ORIGIN      
        1 atggctaggc ctgagccttt tcgagtatac tctcgacggc ccaccgtttg acgcggctca
       61 gcggctgagc ttcgacaatc tgaacaagat cgcatgtctt ggccacatcc gctggattgt
      121 gagctttgta tttgtgcgag cggcgaatca ctttgctgta cagccggtgc cgctttaatt
      181 gatccacggt aaccacgacg gttttctgca ttttatcgct ggtcacacga ccgacgagac
      241 gcttgcgctg cttattcatt tcgattctcc ttgagccagc gcggccagtt cccgctcgcg
      301 caggaccgtc tgcaagcgag cgacatcacg gcggagcatg gtgatccgat tgtgatcttc
      361 cagccgaccg gaagcccact gaaatcgtag gttaaataat tcctgatgag acgcagccag
      421 cttatgctgg atttcttgtg tattcagggc gcgcagttca gttaccgtca tgaccttact
      481 ctccactcat cgaagctaga tcgcggcggc caatgatctg tgtcttgatc gacagcttat
      541 aagccgctaa acgcagcgct tcgcgtgccg ttgcatcatc gatcccgccg atttcaagaa
      601 tgatgcggcc cggcttgata acggccacat agtgatcgac cgcgcccttg cctttaccca
      661 tgcgagtctc ggcagctttt tgagtcaccg gtttgtccgg gaaaattcgc acccacatct
      721 tgccccggcg tttcacgaaa tgcaccaccg cgcggcgagc tgcttcgatc tgtcgctgag
      781 tcatccagca cggctccaaa gcctgcaggc ctaaatcacc aaacaccact tcaacgccgc
      841 gcagttcttt acccttcatg cggccgcgtt gttgtttgcg ccacttcacg cgcttcggca
      901 tcaacatagc ttcacactcc cagccttatt cttcaacggg cgcaatgcga gccgtccgtt
      961 gttctttcgc ttcatttaga atctcgccct tatagatcca cactttgacg ccgatgcggc
     1021 cgtaggtagt aagcgcttcc gatcgggcaa aatcgatatc cgctcgcaac gtactgcgtg
     1081 gtacgcgacc ttccatcacg gtctcacgcc gggccatttc cgatccggct aatcggcccg
     1141 cacaagtaat cttgatacct tcagcaccca cccgcattgc ttgctggatg gcgcgtttca
     1201 tcgcgcggct gtggctgatg cggcgctcta attgattagc aatattgtca gccaccaagg
     1261 aggcttcgag atccggctta tcaatctcgg cgatctccaa tttgactttc ttaccggtat
     1321 tcgcttccag actctcgcgc aactgtttga ccatggcacc tttgcggcca ataatcacac
     1381 caggcttggc ggtatgcacc gttacaataa tctgattcgg gaaacgctca atcgcgatct
     1441 tagagacacc cgcttgagca gtttctttgc gaatgtaatt gcgcagtctg taatcttcga
     1501 ccaaaaactg ggcatactgc ttgccctttt tggcgaacca gcgcgcattc caatctcgaa
     1561 tgacgcctaa tcgaaaacca tatggatgaa ctttacgacc caagtgtcct cctcgtcaat
     1621 cccacgaatc gcgctaggtg ctgactcgtt cgctcaatac caccgtgata tgcgacgatc
     1681 gtcgcaggat cggcttgaac cgaccgcggg ctccaaagcg gcgccattgt cggcgcggac
     1741 cttcatccgc cttgatttca ctgatatata aatcggtgcg tgacaaacct aagttctctt
     1801 ctgcattcgc caccgcggaa acgatcaatt ttaagatcgg ctctgcggcg tggttgggca
     1861 agaacttcag cacggccaag gctttgtcag cctgtagacc acgcacttga ttgatcacta
     1921 atcgaacttt gcggggcgac acttgaacaa agcgcgcaac cgcgtgagca tctgtctgaa
     1981 ccgccatgag cgtttctcct acttcgcagc tggagcggct tcgaccttgg tcgtcgctgc
     2041 ttcttctttc tctcgaatcg tgtgaccgcg gaacgtgcgg gtcggagcaa actcaccgag
     2101 ccgatgaccg accatattct cagtaatgta aatcggcacg tgacgtcggc catcgtgaac
     2161 cgccagtgta tggcctacca tttgcgggaa gatggttgaa gcgcgtgacc aagtgcggat
     2221 gactttcttg tcaccggttt gattcatggc ttcgactttc ttcaaaagtt tggccgatac
     2281 aaacggtccc ttctttaatg atcgtgacat ctcaacctcc gcttaaccta gcgcttcttg
     2341 ccgcgtcggc gcacaatgaa cgaattacta actttgttgc ggcgcgtctt gtaacccaga
     2401 gtcggcttgc cccacggact cttcggaccc ggcataccga cacctgtgcg accttcacca
     2461 ccgccgtgcg gatggtcgcg ggggctcatc gccgagccgc gcacagtcgg gcgaatgccg
     2521 cgatgccgcg atcgaccggc tttgccaagt ttcacgttac cgtgatcaac attgctgacc
     2581 tggcctacaa ccgcaaagca ctcttgtcga accttgcgca tttcacctga aggcagtctc
     2641 aaagttgcgt aatcgccttc tttcgccaac aattgggctg agacacctgc cgcccgagcc
     2701 atctgcgcac ctttgccagg cagcagttcg acattgtgaa ccatcgtgcc taacggcatg
     2761 ttggctaatg ggagacagtt gcccggccgg acttcagctt tcgagccgct catcaccgaa
     2821 tcaccgacgt gaagttccac aggcgcaata atgtaacgct tctcaccatc cgcgtaagtt
     2881 agtaacgcaa ttcgcgccgt ccgattagga tcatattcga tcgtagcaac cttcgccgga
     2941 atattgtgct tatcgcgcat gaagtcgacc aggcgatagg tctgcttatg accaccgcct
     3001 tgatgtcgaa cggtgatccg gcccgtcgaa tttcggccgc catgcttgcg cttatcagac
     3061 aacagcgagc gctcgggagt gctcttcgtg atttcttcaa atgaatcacc ggtcatgcca
     3121 cggcggccag gtgaggtggg tttgtatttt ttaatcggca cgctaataac tcctaggatc
     3181 gatcggccat ttcgttcagc cagaaagatc ggactacacg ccttcaaata gttcgatctg
     3241 ctgatcggcc tgcacggtca cgatcgcttt tttccagccc gcgattcgaa tgcgctgttg
     3301 cgctttcttg ccggttgccc ggctgcgcgg attgcgccgg cgcttggacg gttgattcaa
     3361 aatgtgcacg gcgaccacat tgactttgaa gattttttca acggcctgct tgaccgcttc
     3421 tttgttggcg cgcaagtcca cttcgaaggc atactgatgc agctctttca gatacatggt
     3481 cttttcagtt tgcaatgggc gcttcagcac ttcataaaca tggagttcca tccgtcacct
     3541 cttatcccaa catactctca atgacatcta aggcgccgcg cggcagtatg agcgtgtcgc
     3601 agcccaacaa gtcacgaata ttcaaatact gcgcccgcaa atatttcacg ccggcgaggt
     3661 tccgtaccga tcgctcgaca ttgtcattgt gctcaggcag caccaccaaa gtcgagcgac
     3721 ctgcttcaat gttattcagc acagatgaca tttcttttgt cttggccgaa tcgaattcca
     3781 atttatccag gaccacaata tgttgatcgg ccgctttcac actcagtgct gatcgcaaag
     3841 cggcgtgccg catcttgagt ggcatttgct gcgtatactt gcgcggaatc gggccgaaga
     3901 caatgccgcc ttttcgccac tgcggagcct tgcgactgcc ctggcgcgca cgacccgtgc
     3961 ccttttgttt ccacatctta cgagtgctgc ccgccacttc gctgcgtgtc ttggttttag
     4021 cattaccttg tcgggcattc gccaattgac gagtcaaggc ctgatgcatc aagggcacat
     4081 tgacgggcac ttcaaagatg acggaattga gttcgacttc gccgatgttt tcaccggcca
     4141 tatttttcat tggaatcttc atcgctatac tccgagctgt tgcgcaaatt tgcaacgtgc
     4201 gctacttctt gcgtgtctcc cgaaccaaga ccaaaccgcc cgtggcaccc ggcactgcgc
     4261 cgcgtacgcc caatagattg cgatcggcat caagcaatac gacttgcaaa ttctgcgaag
     4321 ttacgcgctc gacgcccata tggcccgcca tgcgcttgcc tttatgaatc ttgccgggcg
     4381 aagtgcctgc accgatcgat ccggaggtgc gcagtcggtc ggattgaccg tgcgtacgcg
     4441 gaccgcctct gaaatggtga cgcttgacga cgcccgcaaa accgcgacct tttgaagtgc
     4501 caatgacatc cacacgctca cccatcttga ataaatcgac ggtcagtttt tggccttcag
     4561 tataaccttc atccgatttc gcgcgcagtt cgcgcagatg cttaacggcc ggcacttctg
     4621 cggcgagttc tttgcgcttg ggatgcttgg cattcgattt caccaaacct aagtgaccct
     4681 gttcgccacg gctcaatttg gtcatcttct taacggcatc aaaaccgatt tgaatagccg
     4741 aataaccttc tttagcggtt gtacgaactt gcgtcacata gcatggcccg gcttcgatca
     4801 cggtcactaa cagtgattcg ccgccctcat taaaaatttg tgtcatgccg agtttacggc
     4861 ctaaaagacc cttcatgctg cctccacaat gatggggact gtcagtcggg taagctggct
     4921 catcccacaa tcgatcttgg agcaatctgc tcccgatcga tctgccaagc cataagtcct
     4981 taaagattaa tttcgatatc gacacctgcc ggcaaattca ggcgcatcag cgtgtcgatt
     5041 gtcttcgaat ccggatccag cacatcgatc aggcgcttat gcgttcggat ctcgaaatgc
     5101 tcctggctgt tcttgtcgat gaaggacgca cgctgaaccg tgaagcgttc cagccgcgtc
     5161 ggcagcggta ccggaccgac aacagttgcg ccggttcgtt cggctgtctc gacaattctc
     5221 tgcgcggatt gatcgagcac gcgatgatca tatgccttga ggcgaattcg aattttctgc
     5281 ttggccataa tctactgatt ccttaatccc cgcactcaca aaccgtgcga cgtagaaaat
     5341 ctccacgtcg cacgtcatag atctagtccg cgaccttagt aatgacgccc gcgccgacgg
     5401 tcaagccgcc ctcccgtatc gcaaagtgcc cgcccgcttc cagcgccacc ggcgtgatca
     5461 actccacact catcttgatg ttgtcgcccg gcatcaccat ctccacgccc gccggtaagg
     5521 tgatcgtgcc cgtcacgtcc agcgtccgaa tgtaaaactg cggccgatag ccacttacga
     5581 acgctttgtg ccgtccgcct tcttccttct tcaagacgta cacttccgcg tcaaacttcc
     5641 ggtgcggctt gatgctgccc ggcttggcga gcaccatgcc ccgctccaca tccgtccgct
     5701 cgatgccgcg caacagcaga cccgcattgt cgcccgcttc ggcatcgtcc aacgtcttgt
     5761 ggaacatttc catcgaggtc acaaccgtat cccgcacttc atcgcgcagg ccgacgatcg
     5821 agatcggatc gcccggtttg atcacgcccc gatcgacccg gcccgtcacg accgtgccgc
     5881 ggcctttgat cgagaacacg tcttcgaccg acatcatgaa cggcttgtcc gtctcgcgct
     5941 tgggcgtcgg aatgtattcg tcgacgacgc gcatcaagtc ccagatcggt ttgtaggccg
     6001 gagcattcgg atcggtgctc ggttcgttca aggccgccag ggcgctgccc cgcacgatcg
     6061 gggtctcgtc gcccggaaag ccgtaaccgt tcaacagttc gcgcagttcc aactcgacca
     6121 gttcgagcag ttccggatcg tccatcatgt cgactttgtt caagaagacg acgatggccg
     6181 gcacttccac ttgcctcgcc agcaggacgt gctcgcgcgt ctgcggcatc gggccatccg
     6241 gcgcggccac aaccagaatc gcgccatcga cctgcgccgc gccggtgatc atgttcttga
     6301 tgtagtcgcg gtgaccgggc atgtcgacgt gggcgtagtg ccgcttctcg gtctcgtatt
     6361 cgacgtgggc aatgttgatc gtaatgccgc gctctttttc ttccggcgca ttgtcgatcg
     6421 agtcgaacga gcggaacgtg gccagtttct tcaggcctaa gaccttggtg atggccgccg
     6481 ttaaggtggt cttgccgtgg tcgatgtgac ccatggtgcc cacgttgacg tgcggcttgc
     6541 tgcgatcata gtgctgcttc gacatgatgt tgtcctccta tagtctttca gcgcatagcc
     6601 cgagtgggtt cgcgcttgcc atttttcaaa atttcttcgg caacactttg cgaaacttcc
     6661 gcatagtatt caaattccat cgtgaatgtg ccgcgacctt gagtgctcga tcgcaaatcg
     6721 gtggcgtaac cgaacatgtt gcccaatggc acgtgcgact tgatcgcttg agcattaccg
     6781 ggccgtggtt ccatgccttc gatctggcca cggcgggcag agaggttccc gatcacatcg
     6841 ccggtgtatt catccggcac aaccacttcg actttcataa tcggttcaag caaaaccggt
     6901 ctggcttttt gcgcgccatc tttcagcgcc atcgaagccg caatcttgaa ggccatttcg
     6961 tttgaatcca cttcgtggta ggaaccatcg atcaacgtcg ctttgatgtc caccatcgga
     7021 taaccggcga ccacgccaga ttccatcgct tctttaatgc ccttttcaac ggctggcaca
     7081 tattcccgcg gaacggtacc gccgataatg ccattctcga attcaaagcc tttgcctttt
     7141 tcgagcggct ccagttgaag ccaaacgtgt ccgtactgac cacgcccgcc ggactgtcga
     7201 ataaagcggc cttcgatctt aactggccta gtgatcgttt ctcgatacgc gactttaggc
     7261 cgaccgacat tcgcttgaac tttaaattcg cgcatcattc gatcgaccaa aatctccaag
     7321 tgaagttcgc ccattccagc gatcaacgtc tgagcggtat tctcgtccgt gcggacgcga
     7381 aatgtcgggt cctcttcagc taatcggcgc agcgcttcac ccatcttgtc ctgatcggca
     7441 gccgtcttgg gctcgatcgc aatttcgatc accggatcgg ggaaagagat cgattcaagt
     7501 atcactggtt tatcaatatc gcacaacgtg tcgccggtga aagaatcttt caaggctaac
     7561 gtcgcgccaa tatcacctgc gtagaccacc gtttgctcga cacggcgatc ggcatacatg
     7621 cgcagcacgc ggcctagtcg ttccttgcgg cgcttgctgg aattcagaac cgtctcaccc
     7681 acatcgacct tgccggaata aacacggaag taggccaagc gcccgacaaa cggatcggcg
     7741 acaatcttaa agaccaaagc gcagaacggt tggccctcgt ccgtggtgcg aacaaccgat
     7801 tcgccggtat ccggatcgag acccgtcacc gatggaacat cgatgggcgc ggggagataa
     7861 tcaatgacag catccagcat tggctgcacg cctttatttt tcaggctgga accgcagaac
     7921 actggaacaa ttttgcctgc tagggttgcc ttacgcagcg ccttcttgag ttcatcaacg
     7981 gagagggttt caccttcaag gaaacgatga gtcaaatcat cgtctgtttc ggcgatacgc
     8041 tctaagaggg tttcatgcgc cgccttcgcc tcagcttgca tgtccgccgg gatgtcacgc
     8101 gtatccattt tcaagcccag atcatcggtc caatagatcg cgttcatttc catcagatcg
     8161 accacggcac gaaaatcaga ctcatgcccg atcggcagtt gcagtggcag cggattcgcg
     8221 ccgaggcgat ctttgatcga ggccacggcc cgatagaagt cggcaccagt acgatctagc
     8281 ttattgatga agcaaatacg cgggactttg tagcgatcag cctggcgcca aactgtttca
     8341 gactgcggct caacgccttg gacgccatca aaaacaacaa caccgccatc cagtacgcgc
     8401 aatgatcgct gtacttcggc ggtgaagtca atgtggccgg gagtatcgat gatgttgatc
     8461 tggtattcgc gccattcgca cgtaatcgcg gcggccgtga tcgtaatacc gcgctcgcgt
     8521 tcctgctcca tccagtcggt gacggtcgtg ccgtcatcca cgctgccaat ccgatgggtt
     8581 ttgccggtat aaaacaaaac ccgctcggta gttgtagttt taccggcgtc aatgtgggca
     8641 atgatgccaa tattgcgcat gagttcgagc gggaattccc gtgtcatttc aacaacactc
     8701 tctacggttc ttgaggcgcc aggtttcggt caacctgagc tgatcggctg agccgacgcg
     8761 ctggacacca agtgagaaca atatacgctt tacaaattta ccatcggaag tgagcaaagg
     8821 cccgattggc ttcggccatc ttatgcgttt cgtctttctt cttgatcgcg gcaccgttgc
     8881 cttgcgcggc gtcgatcaat tcgcccgcta acttctccat catcgacttg ccgccacgtg
     8941 aagtcgcagc tgccagtagc cagcgcatcg ctaaggtatt gcgtcgatcg tttggcactt
     9001 cgactggcac ttgatatgta gcgccaccta cccgtcgagg ctttacttcg atctgcggcg
     9061 tcgcgttctt catcgcctgc tcaaaaacct cc
//
LOCUS       DASRTJ010000410         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_25508,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000410 DASRTJ010000000
VERSION     DASRTJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25508"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFF70_08365"
     CDS             complement(<1..187)
                     /locus_tag="VFF70_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZY44748.1"
                     /translation="MTARNMCEETQLANKLIMTGEGADELLGGYHWFDGDRRARKFLR
                     WPQFLRSTLAHAPINIST"
     gene            347..1111
                     /locus_tag="VFF70_08370"
     CDS             347..1111
                     /locus_tag="VFF70_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZY44749.1"
                     /translation="MLRIATYNLYLGGTDRVEAIHSVLSSIDADVIALTEADDRSVVE
                     ELANRLKMHFVWEHGSGDRHIATLSRFPILASNIYRTSPLTQAVLETKVDVGKQTLTL
                     YNAHFLPYLLLPFEVRRWQAAGKLLSIIRSKPNEPHIILGDLNSIAPGDRPLQRNNPP
                     RMRRVMLLQLRLIFRLALPRLLRAGYLDCFRSLHPRDDGFTWMTGNRTTRFDYILANP
                     IMARALRTCRVFDGHPALERASDHYPLVAEFDLEQL"
     gene            1108..2181
                     /locus_tag="VFF70_08375"
     CDS             1108..2181
                     /locus_tag="VFF70_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44750.1"
                     /translation="MISESFVREHLDRFEDFWHRREAAIQHEVTIEAYGYPLHFTTND
                     PLLLEVPRLCADRYSRCNPIESAPALRLNFMLDRQLPSKRVPINWPSRLRYSSQGDWL
                     TVNVEPWVNSFADLKQQIGLAFVSESIAKKPLLLSRYIGDCFVLNMLLRTGWGELHAS
                     GLTRDGRAILLIAPHNTGKSTTAFRLAMNGYQLLSDGMTYVRHHTPHPNPPPSQKADR
                     RGDIELLGYPIGEVKLRMDMLNEFPQIKKHGRKALVREDTKMVFNLREAMPDRMCAES
                     IWPQEIVVCLLERSNTRRTTTEPIDPQAALIDLLANSSHRNEPELMAVNLNSIEQLLD
                     GARCYRLKIGSDVAGILKVVETL"
     gene            complement(2202..>2628)
                     /locus_tag="VFF70_08380"
     CDS             complement(2202..>2628)
                     /locus_tag="VFF70_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44751.1"
                     /translation="GLAALTDRTIGRRVTTGLIALFVAVDIGAMFFIPRPIEYRAAGL
                     ALRGQIPANAPVLMRKRQIAFYSNTAYEWIPFSDVQGVLDYGQTKGADYFMIDSATTL
                     ATRPQLVYLLDETNAPSLIRVFYKTGGDTDKVIVYQIVK"
BASE COUNT          583 a          736 c          691 g          618 t
ORIGIN      
        1 cagttgagat attgatcggc gcatgcgcga gcgtgcttcg caagaattga ggccagcgca
       61 gaaattttcg cgcgcgtcga tcgccgtcga accagtgata gccgcccagt aattcatccg
      121 cgccttcgcc ggtcatgatc aatttattcg ctagctgcgt ttcctcacac atgttgcgtg
      181 ccgtcattgc gagcgaagtc cctagatggc acagccctct tgggatgtgc ggcgaagcaa
      241 tctcgttgac tattgtggag attgcttcgt cgcaaaagac actcctcgca atgacgcctg
      301 aactgcgccg aatgctttac ttattacttg ttactcgtga cttataatgc tccggatcgc
      361 cacctacaac ctctacctcg gtggaaccga tcgagtcgaa gcgatccatt ctgtgctgtc
      421 gtcgatcgat gcagacgtga tcgcgctcac cgaagcggac gatcgatccg tggttgaaga
      481 gttagcgaac cgattgaaga tgcactttgt ctgggaacac ggcagcggtg atcgacatat
      541 cgccaccttg tcacgctttc caattcttgc atcaaatatc tatcgcacgt cgccgctgac
      601 acaagcagtt ctagaaacta aagtggatgt gggcaagcaa acgttgactc tttataacgc
      661 gcacttcctg ccatacttac tgttgccttt tgaagtccgg cgctggcaag ccgccggcaa
      721 gttattgagc atcatccgat cgaagccgaa cgagccacac atcattctgg gagacctcaa
      781 ctcaatcgcg ccgggcgatc gcccgcttca acgcaacaac ccgccgcgca tgcgtcgtgt
      841 tatgctattg caactgcggc tgattttccg attggcgctt ccgcgtttgc tgcgggcggg
      901 ttaccttgat tgctttcgat cgctgcatcc acgcgacgat ggctttacgt ggatgaccgg
      961 caaccgcacg acgcgcttcg attacattct ggctaatccg atcatggcgc gcgcgctgcg
     1021 aacttgccgc gtcttcgatg ggcatccggc gcttgaacgc gcatccgatc attatccgct
     1081 ggtggccgaa ttcgatctgg agcaactgtg attagcgaat cgtttgtgcg tgaacacctc
     1141 gatcgctttg aggatttctg gcatcggcgc gaagcggcca ttcagcacga agtcacgatc
     1201 gaggcttacg gctatccgct gcacttcacc accaacgatc cgctcttgct ggaggttccg
     1261 cgcctgtgcg ctgatcgcta ttcgcgctgt aacccgatcg agagcgcgcc agctctccgt
     1321 ctgaacttta tgctcgatcg gcaattgcca agcaagcgcg tgccgatcaa ttggccatcg
     1381 cgcttgcgct acagcagtca aggcgattgg ctcaccgtca atgtcgagcc gtgggtgaac
     1441 tcattcgctg atctcaaaca gcagatcggg ctggccttcg tttctgaatc gatcgcgaag
     1501 aaaccgctgc tgctcagccg ctatatcggc gattgctttg tgttgaatat gctactgcgt
     1561 accggctggg gtgaactgca cgcgtcgggc ttgactcgtg acggtcgcgc aatcttgttg
     1621 atcgcgccgc acaacaccgg caaatcgacg accgcgtttc gattggcgat gaatggttat
     1681 cagttgttgt cggatggcat gacatacgtc agacatcata ccccccaccc taaccctccc
     1741 ccgtcgcaaa aagccgacag gcgaggggat attgaacttc ttggttaccc gatcggtgaa
     1801 gtgaaactgc ggatggatat gctgaatgaa tttccgcaaa taaaaaagca cggccgcaag
     1861 gccttagtgc gcgaagatac caaaatggtt tttaatctgc gggaggcgat gcctgatcgg
     1921 atgtgtgcgg aatcgatttg gccgcaggag attgtcgtat gtttattgga gcgatcgaac
     1981 acccgcagga caactaccga accgatcgat cctcaagctg cattgatcga tctgctggcc
     2041 aactcctcgc atcgcaacga gcctgaactc atggcggtca acctcaactc gatcgagcag
     2101 ctgcttgatg gggcgcggtg ttatcggctg aagatcgggt cggacgttgc aggaatcttg
     2161 aaagtagttg agacattgtg aagcgccgaa gcactttcga tctacttcac aatttgataa
     2221 acgatcacct tgtctgtatc gccgccggtt ttgtaaaaca ctcgaattaa tgacggcgca
     2281 ttggtttcat ccaaaagata gactaactgc ggccgcgtcg ctagggtcgt ggctgagtcg
     2341 atcataaaat aatccgcacc tttcgtctgc ccgtaatcta aaactccctg cacatccgaa
     2401 aacgggatcc actcataggc cgtgttggaa taaaacgcga tctgtctttt acgcatcagc
     2461 accggcgcat tcgctggaat ttgtccgcgc agggcgagtc cggcagcgcg atactcgatc
     2521 ggacgcggga tgaaaaacat cgcgccgata tcaaccgcaa cgaacaatgc gatcaaccca
     2581 gtcgtgaccc tgcgcccgat cgttcgatct gtcaaagccg ccagtccg
//
LOCUS       DASRTJ010000411         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_100831,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000411 DASRTJ010000000
VERSION     DASRTJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100831"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFF70_08385"
     CDS             complement(<1..266)
                     /locus_tag="VFF70_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44752.1"
                     /translation="MLTHTLLIESNDPIIAAAGLIYRVRRGSIGAPLIVMIHGLGGDE
                     NVMWIFERALPANATVLSPRAPIWIEPNTPYAEEASGGYSWLRSA"
     gene            340..1194
                     /locus_tag="VFF70_08390"
     CDS             340..1194
                     /locus_tag="VFF70_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY44753.1"
                     /translation="MKLVTDSAVDLPEEMFGQYEIERMPLLISFGHESLRSGIDIQPE
                     EFYRRLAASTDLPTTSQPSVGEFVDLYRRIGQSDKDILSLHISSGLSGTINSARLAAQ
                     QCPDLKITLVDTKTLSAAQGFQIVAAAQAIRRHLPLDQILARIQQVADQTEIFYTLDT
                     LHYLQKGGRIGRVQAMAGSLLHLKPVITVDKDPGTYIRAGNTRSLAKSMVMIADLIQM
                     KVKDYPAWVHVLHSNTLEHAHVLAELITARLPGAKITNGQIVPVLGVHVGPGCVGAAC
                     GPADILAA"
     gene            1260..1712
                     /locus_tag="VFF70_08395"
     CDS             1260..1712
                     /locus_tag="VFF70_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZY44754.1"
                     /translation="MATKKKVTRKITKSTKPRSVAPRATKRTVISTELAPKAIGPYSQ
                     AIRAGDFIFTAGQVPIDPATGQLIDGDISAQTRRALMNVSAILDAAGTSMSKVVKTTV
                     FMANMNDFSLMNAVYAEFFPEDPPARSTFGVAALPRNALIEIETITLA"
     gene            1740..2414
                     /locus_tag="VFF70_08400"
     CDS             1740..2414
                     /locus_tag="VFF70_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY44755.1"
                     /translation="MSKETILIVDDEQNIIELARLYLEQEGFRVESANTGRAALDKVK
                     AVDPALIVLDLMLPEIDGWEVCRRVRSNSDVPIIMLTARDEDVDKIVGLEIGADDYMT
                     KPFNPRELVARVKAILRRSERAAMPGKAIHIGDLSIDPARREVLVAGQPIDVRTKEFD
                     CLMTLAENKGIVLSREKLLELAWGYDFAGQTRTVDVHVAHLRKKISHSTAQIETVTGV
                     GYKLVG"
     gene            2515..3138
                     /locus_tag="VFF70_08405"
     CDS             2515..3138
                     /locus_tag="VFF70_08405"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZY44756.1"
                     /translation="MTAKQLTIEQIRDAFKRPLPGLEAQIKLAPEIRIKELRTPQPLD
                     AKPAGVLILLYPHRGQLHFPLIHRTSNGHDRHSDQVSLPGGSQEANESIQRTALREME
                     EEIGVTRDQINIVGTLSTIYIPPSNFLVTPTVGYVDHRPDFHCDTHEVAELVEVPLAT
                     LFDPASIRREEWELRGTKVEVPFYLIGPHKVWGATAMILSEFSMLLS"
     gene            complement(3130..3582)
                     /locus_tag="VFF70_08410"
     CDS             complement(3130..3582)
                     /locus_tag="VFF70_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44757.1"
                     /translation="MRALPEATRTKLPKKLQPFKISLRPWLVQFYYTDPLLHYEVVTL
                     GDRRGVLEIGLHFESRTSETNAWLLEGFMRHLFEIKIELRAQIEAEVWDKGWTKVYET
                     IDLEPFDEAYLEHVAGRLAKLITVLQPIFERVIKDQKKSRHSSGAFLR"
     gene            complement(3603..>3801)
                     /locus_tag="VFF70_08415"
     CDS             complement(3603..>3801)
                     /locus_tag="VFF70_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZY44758.1"
                     /translation="FNTVAIGNGANDVEMLKAAAIGIAVLGREGTASEAIAAADIVTL
                     NVIDAIDLLLNPKRLVATLRR"
BASE COUNT          903 a         1050 c          985 g          863 t
ORIGIN      
        1 gccgatcgga gccagctata accgccactg gcttcttcgg catacggcgt attcggctcg
       61 atccagatcg gcgcgcgagg actgagcacc gttgcatttg cgggtaacgc gcgctcgaag
      121 atccacatta cgttttcatc gccgccgagt ccgtggatca tcacaatcaa cggcgcaccg
      181 atcgatccgc gccgcacccg atagatcagg ccggcggcag cgatgatcgg atcattcgat
      241 tcgatcaata gagtgtgagt cagcatgtcg tgattataac ggaatgatgt acaatgtgaa
      301 gggccatggg agaggcaatc gatcgcatgg agaattggta tgaagcttgt aacggatagc
      361 gctgttgatc tgcctgaaga aatgttcggc caatatgaga ttgaacgcat gccgctgttg
      421 atctcgttcg gccacgagag tctgcgcagc ggcatcgaca ttcagcccga agaattttat
      481 cgccggctgg cggccagtac tgatctccct acaacttcgc agccctcggt cggcgagttt
      541 gtcgatctct atcgccgcat cggccaatcg gataaagaca ttctctcgct gcatatttca
      601 tccggcctga gtggcacgat caattcggct cgactggccg cgcagcaatg ccccgatctg
      661 aaaatcactt tggtggatac gaaaacatta tcggccgcac agggttttca gatcgtcgcc
      721 gccgctcaag cgattcgacg ccacctgccc ctcgatcaaa ttttggcgcg cattcagcaa
      781 gtcgccgatc agaccgaaat tttttacaca ctcgacacgc tacactattt acaaaaaggc
      841 ggtcgcatcg gccgcgtgca agccatggct ggctcactat tacatcttaa gccggtcatc
      901 accgtcgata aagaccccgg cacttacatt cgcgccggta atacgcgcag tctcgccaaa
      961 tcgatggtga tgatcgccga tctcattcaa atgaaggtga aagattatcc tgcctgggtg
     1021 cacgtgctgc atagcaacac gcttgaacat gctcacgtcc tagctgaatt gatcaccgcg
     1081 cgtttgcccg gtgctaaaat cacgaacgga cagattgtgc ccgtgttggg tgtgcacgtt
     1141 ggccctggtt gcgttggcgc agcgtgcggt cctgctgaca tactggcggc gtgattaatt
     1201 gggaacttga gtgaggataa ggtagaatcg cgctacattc attatcaagg agatcgatca
     1261 tggccactaa gaaaaaagta actcgtaaga tcactaaatc cactaaaccc cgatcggttg
     1321 cgccgagagc gacgaagcgc acggtgatct cgaccgagct cgcgcccaaa gcgatcgggc
     1381 cgtattctca agccattcgc gccggcgatt ttattttcac ggccggccaa gtcccgatcg
     1441 atccggcgac cggccaatta atcgacggcg acattagcgc acagacgcgc cgcgccttga
     1501 tgaacgtcag cgcaatcttg gatgcggccg gaacatccat gtcgaaagtc gtcaagacca
     1561 cagtcttcat ggcgaacatg aatgacttca gtctgatgaa tgccgtgtat gcggaattct
     1621 ttccagagga cccgcccgct cgatcgacct tcggcgttgc cgcgctcccg cgcaatgcct
     1681 tgatcgaaat tgagacgatt accttagcgt aggggcgagg ccgcctcgtc catttccaca
     1741 tgtccaaaga aaccatcctc attgtcgacg acgaacagaa catcatcgaa ctcgctcgac
     1801 tgtatttaga acaggaaggc tttcgcgtcg agtcggccaa cacgggcagg gccgcgctcg
     1861 ataaagtgaa agctgtcgat ccggcgttga ttgtgctgga tttgatgctg cccgagatcg
     1921 atggctggga ggtctgccgc cgtgttcgat caaatagcga tgtgccgatc atcatgttga
     1981 ccgcgcgcga tgaagatgtc gataagatcg tcggcctgga aatcggcgca gacgattaca
     2041 tgaccaagcc cttcaatccg cgcgaactcg tggcacgcgt caaagcgatt ctgcggcgat
     2101 cggaacgcgc cgcgatgccg ggcaaagcca tccacattgg cgatctctcg atcgatccgg
     2161 cgcggcgaga ggtgctggtc gctggccagc cgatcgatgt gcgcacgaaa gagtttgatt
     2221 gtctgatgac gctggccgag aacaaaggca tcgtcttgag ccgcgaaaaa ttattggaat
     2281 tggcctgggg ttacgacttt gccggacaaa ctcgcacggt cgatgtgcat gtggcgcatt
     2341 tgcgcaagaa gatcagtcac agcaccgcgc agattgaaac ggtgacaggt gtaggttaca
     2401 agttagttgg ctagaccaga attttctaac cactaagaca cgcaggcgca aagtttatat
     2461 ttgatgcttc gtgtctttgt gtcttcgtgg ttatgatcga atcattaaaa tataatgaca
     2521 gcaaaacaac ttaccattga acaaatccgc gatgccttca agcgtccgct gcccggcctc
     2581 gaagcacaaa tcaaacttgc acccgaaatc cgaatcaagg aacttcgcac cccccaacct
     2641 ctagatgcaa aacccgccgg cgtgctgatc ctgttgtatc cccatcgagg ccagttgcat
     2701 ttcccattga ttcatcgcac gtccaacggc cacgatcgac atagcgatca agtttccctg
     2761 cccggcggat ctcaagaagc gaacgagtcg atccaacgca cggctctgcg tgaaatggaa
     2821 gaagagatcg gcgtgacgcg cgatcagatc aatatcgttg gtacgctctc gacgatttat
     2881 attccaccca gcaacttttt agtgacgcca actgtgggct atgttgatca tcgacctgat
     2941 tttcattgcg atacacatga agtagcagaa ttggtcgaag tgccgcttgc aactttattt
     3001 gatccagcat cgatccggcg cgaggaatgg gaattgcggg gtacgaaagt cgaagtgccg
     3061 ttctacctga tcggcccgca caaagtgtgg ggtgccacgg cgatgatcct cagtgagttc
     3121 agtatgcttt tatcgtagaa acgctccgct ggagtgtctc gattttttct gatcctttat
     3181 caccctctca aagatcggct gtaacaccgt gatcaacttc gccaatcgcc ctgcgacatg
     3241 ttccaaataa gcctcatcga atggttccag atcgatcgtt tcataaactt tggtccagcc
     3301 tttgtcccac acttccgctt cgatctgagc cctcagttcg atcttgatct caaagagatg
     3361 ccgcataaaa ccttcgagca gccaagcgtt ggtctcgctg gtgcggctct cgaagtgtaa
     3421 gccgatctcc aaaacgccgc gccggtcgcc aagcgtgaca acttcataat gcaataacgg
     3481 atcggtgtaa tagaattgca ccaaccacgg tcggagacta attttgaaag gctgtaactt
     3541 cttagggagt tttgtgcgcg tggcctcagg caatgcgcgc atgaagtcgc ccgatcgcat
     3601 cctcatcgcc gcagcgtcgc caccaaacgt ttggggttga gcaacagatc gatcgcatcg
     3661 atcacgttca gcgttacaat atcggccgca gcgattgctt cactggctgt gccttcgcga
     3721 cccaagaccg caattccgat cgcagccgct ttcaacatct ctacgtcatt cgcgccgtta
     3781 ccaatcgcca ccgtattgaa t
//
LOCUS       DASRTJ010000412         8596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_63692,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000412 DASRTJ010000000
VERSION     DASRTJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63692"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VFF70_08420"
     CDS             complement(<1..1394)
                     /locus_tag="VFF70_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZY44759.1"
                     /translation="MLPDIELLISQLTLEEKVLLLAGADLWHTNPIERLGIPVLKVTD
                     GPNGARGSEETTGITSVCFPSGIALAATWNTELVERVGQALGDETRSKGAHLLLAPTV
                     NMQRTPLSGRNFEFYSEDPYLSGRMATAYILGLQSRGVGACIKHFVCNESEFERQTIS
                     SVVTERALREIYLKPFEMALRAAKPWAFMSAYNKINGTYASENAYLLKDILKGEWQFD
                     GLVISDWFGTYGPGVVGNGLDLEMPGPARWMGEPALEAVRSGRVSEAKINDSVRRLLR
                     TLQRAGAFEQPELQPEQAIDLPEHRQLVRAAAAEAIVLLKNADDILPLAADRITSIAV
                     IGANAQQPAIIGGGSASVTPHYIIKPVEAIAQHAGSSIKVEFETGCPLHKMMPLLDTH
                     FLVADDGRSGFAGDVFDNLELVGEPADHLFFDRMAIDFTNRLLAKVNRQSFSARLSAN
                     FTPAETGRYTFGLEG"
     gene            complement(1791..2876)
                     /locus_tag="VFF70_08425"
     CDS             complement(1791..2876)
                     /locus_tag="VFF70_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZY44760.1"
                     /translation="MAETSVTGGSPATPVSRRFRPRMRKSLDSLGVTGVGLLSMVVFL
                     LPLAYVLTTAFKLDSQMTTIGAPLWPAAAATYNYQGTDYPLYDVPTDTGTRQLALVQG
                     YREDSDMVDPAAPEQGVFNVKGRWRTWQAVYQFAPNFKNFTDAWDQVNFPRLFMNSLL
                     LATIGTIGTLISCTLVAYGFARFRLPGKNILFVILISTIVLPPQATIIPLYILFSKLQ
                     WTGTWLPLLVPAFFANAYDVFLLRQYFMSVPKELDEAAMLDGANPLRILVSVIIPQAI
                     PALTAVLIFHFFFVWNQYFEPLVYLLGHEKLYPVSVGIGQFVTTFSTYPGRSAATAVL
                     AMALPAVIFFLAQRQFMQGIVITGVEK"
     gene            complement(2892..3857)
                     /locus_tag="VFF70_08430"
     CDS             complement(2892..3857)
                     /locus_tag="VFF70_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZY44761.1"
                     /translation="MSATTRTILAPAAPRRSISARTRRERVHGFLFLSPWIFGFFAFQ
                     LLPILFTIFLSFTDYKGSSEFKLGNFNFVGLDNYKHLLTDPVALPSMGVTLKFALIGI
                     PLGLIVPLYLAVLVNSKRLRASSFFRTLFYLPSVIPVVAGAIIFNGVMNAQSGWLNLV
                     LKAIGITDPPRWFTDPAWAGLALNLLAIWAVGNAMIILLAGLQGVPTELYEAATVDGA
                     SPLRQFFSISIPLISPVIFYNVTIGVIGAFQYFVPALLIGGTNGDPQGSLLFFPTVFY
                     REAFVFSNMGYASVLAVVIFVVTMSAAGLLFFSAQRLVYYAGGQA"
     gene            complement(3939..5456)
                     /locus_tag="VFF70_08435"
     CDS             complement(3939..5456)
                     /locus_tag="VFF70_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZY44762.1"
                     /translation="MKRNMKLMFALVMVFSFVLSACAPAVAPTTTSGPAPTPVVVVVT
                     KEVIKQVEVTAVPTAAPTGVPTPTIQPEIKTGGEGCDPASTQVTWFIGLGAGSNADVV
                     PLEKAWVDKYNKSQKEACVLMDVVYNTGQNSYDALRALIAGGNAPEIVGPTGKAGRAS
                     FQGAWADVAQLAKDAGFDLSKYDPKLLDFTKDEGKLVGLPFALFPSFIFYNKKLFDEA
                     KLPYPPHKVGELYNGKPWDLAAFQDLAMKLTVDKAGNDATNAAFDPANVTQFGFFAQW
                     TDARGLGAFFDGGIPYDAKTNSAVIPSAWEAAWKWYYDGIWKYHFMPTADYQNSDQFN
                     KGNLFASGNVAMSWVHTWYMCCFDNSKTSWDMAVVPTINGKITAKLHGDTFAILKESK
                     QQAVAFKVLTAMVTDPDLPVIYGGMPAVEADRPAFFAAKDKAAAPNKIDWTVAQAMLA
                     YPDLPNHEAWLPNLAQANTLLGAFRTLMDQTPLLDLDAAIAKLKSDLDAAYKAAK"
     gene            complement(5550..6557)
                     /locus_tag="VFF70_08440"
     CDS             complement(5550..6557)
                     /locus_tag="VFF70_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZY44763.1"
                     /translation="MDLETIAKLSKVSRSTVSRVLNNHPNVSPPTRERVLGIIHKMNF
                     QPNVAARGLAKGYTRVLGLVIPMGVAALFTDPYFPSFIQGVSAACNVHDHSVMLWLAE
                     PEYERRTIRQIMHSGLLDGVIVASNLLDDPVVQALLEGDLPFILNGRHPNDGRVNYVD
                     IDNIASAREAVLHLLRLGRKRIGTIAGPHDMIAGADRLDGYIAALRDRGQLVDSDLIV
                     EGDFTEAGGYSAMQRLLARRPDAVFAASDTMAVGALRALREASLRVPEDVAIVGFDDM
                     SFAAHTDPPLTTVRQPIHRSGELATETLIDLIQHPHSAPRRITLPTELVVRTSCGARL
                     N"
     gene            6885..7526
                     /locus_tag="VFF70_08445"
     CDS             6885..7526
                     /locus_tag="VFF70_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44764.1"
                     /translation="MKKVLIILATIAGVIALGNVLIAGAQNGVVRFNSSMSSMMTNYT
                     ATYTGTMPFGRGMHGDTYTGTVPFGGQMRDQMSQMNQKVLDAVSAKLGMTTSDLLSAL
                     QNRQTLTDLAKAKNVSISDLQSAADAAHQAALDDLVKQNVITQQQADLMLAHMQDMRI
                     LGFGGGGHGFGMPHDMPHGFGPNGSFPGRGMLHSFPGSVQPQQPSVITPSSQG"
     gene            7615..8307
                     /locus_tag="VFF70_08450"
     CDS             7615..8307
                     /locus_tag="VFF70_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY44765.1"
                     /translation="MSHTILVVDDELEIVKVVRAYLEQNGFRVLTSADGQQALMIFRH
                     EQPDLVLLDLNLPSLAGLDVCRSIRRESNVPIIMLTARVEETDRLIGLEIGADDYIVK
                     PFSPREVVARVRAVLRRSIAAPEQPARLSIGDLKIDVDKHEVYLHTQSIDLTPSEFNI
                     LHAMAAQPGRAFSRMDLLDAAQGEAYEGYERTIDVHIKNLRQKLGEDPRNPSFILTVY
                     GIGYKFSDKVKD"
     gene            8346..>8596
                     /locus_tag="VFF70_08455"
     CDS             8346..>8596
                     /locus_tag="VFF70_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44766.1"
                     /translation="MLSFALVVAIGLGAVVLVANQVTNRELHEFMMGPDNDIGADVTP
                     GMMLGRVGPPQLRQPVIDRVNAAIIVGSLLALAAALAIG"
BASE COUNT         2062 a         2367 c         2337 g         1830 t
ORIGIN      
        1 ccttccagtc caaaagtgta gcgccccgtt tcagcgggtg tgaagttggc cgacaggcgc
       61 gctgaaaaac tttggcggtt gactttggcc agaaggcgat tcgtgaagtc gatcgccatc
      121 cgatcgaaaa acagatgatc cgccggttcg ccgacaagtt cgagattatc aaacacgtcc
      181 cctgcaaaac ccgatcggcc atcgtcggcc acgaggaaat gcgtgtcgag cagcggcatc
      241 atcttgtgaa gcggacagcc cgtttcaaat tcaactttta tcgagctgcc ggcatgctgc
      301 gcgatcgctt ccacgggttt gatgatgtaa tgcggtgtca cgctagcgct acccccaccg
      361 atgatagcgg gctgttgcgc attcgcgccg atcacggcga tcgatgtgat ccgatctgcg
      421 gccagcggca aaatatcgtc cgcgttcttc aacaacacga tcgcttcggc ggccgctgcc
      481 cgcaccaact ggcgatgttc cggtaaatcg atcgcttgct ccggctgcaa ttcaggctgc
      541 tcgaacgcgc ccgcgcgctg caaggtacgc agtaaccgcc ggacactgtc gttgatcttg
      601 gcctcgctga cccggcccga tcggacagct tccaacgcag gttcgcccat ccatctggcc
      661 ggccctggca tctccaaatc aagcccatta ccgacgacgc ccggaccata cgtgccgaac
      721 caatcggaga tgaccaagcc atcgaactgc cactcgcctt taagaatgtc tttcagcaag
      781 taggcatttt cgctggcata agtgccgttg attttgttat acgccgacat gaacgcccag
      841 ggtttagccg cgcgcagcgc catctcgaac ggcttgaggt aaatctcgcg cagcgcccgc
      901 tccgtcacca ccgagctaat ggtctgccgt tcaaattcgg attcgttgca gacgaagtgt
      961 ttgatacaag cgcccacccc gcggctctgc aatcccagaa tgtaggccgt ggccatacgg
     1021 ccagagagat agggatcttc tgaatagaat tcaaaattgc ggccgcttaa cggtgtccgc
     1081 tgcatattga cggtgggggc gagcagaagg tgcgcgccct tggacctggt ttcgtcaccg
     1141 agggcttgac caacacgctc gactagttct gtattccaag tggcggccag cgcgatgccg
     1201 ctgggaaaac agaccgaagt gatccccgtc gtttcttcac taccgcgtgc tccattgggg
     1261 ccgtcggtca ctttgagaac gggaatgccc aggcgttcga tcgggttggt atgccacaga
     1321 tccgcgcccg ccagcagcaa gactttttct tcgagcgtca gttgtgaaat caacagctca
     1381 atatcaggca gcatatattt cctttcaatg ctggtaagtc acagtggtaa ttgccgagga
     1441 ggcgcgcacg ggagcaccgg gttgctgaca cgaagcacgg gtcaggaaag ttaccaaatt
     1501 ccgtgtcagc accattcaat ccccaacctg tttctcgacc ccagtaaaga ccacaccttg
     1561 tttaaagatg cgctgggctg cgaagaagat cgcaatcggc agggccaacg tgaggagcgc
     1621 cctgtcttga agaagatgag gctgcgaccc gtacaactgc ttacctatct gcaaacccaa
     1681 caccagcggc tacaaatccg agcatgaagg gcgccgtctt atatgcagat gccagcgtca
     1741 agccgagaac agctagccgt tgtcggtccg ctgtctctcg ttgtggcaca ttatttttcc
     1801 actccggtaa tgacgatgcc ctgcataaac tgacgctgag ccagaaagaa tatcaccgct
     1861 ggcaacgcca tggccaagac agcggtggcc gctgagcgac cgggataagt gctgaacgtg
     1921 gtcacaaact ggccgatgcc gactgaaact ggatataact tttcatggcc cagtaaatag
     1981 accagtggtt caaaatactg attccacacg aagaaaaagt gaaagatcag gacagcggtg
     2041 agcgccggta tggcttgggg aataatgact gagacaagga tgcgcaacgg attagctcca
     2101 tccagcatgg ccgcctcgtc caattccttg ggaacactca tgaagtactg gcgcaacaga
     2161 aaaacatcgt acgcattggc aaaaaaggct ggcaccaaca gcggcaacca ggtgcccgtc
     2221 cactgcaatt tgctgaacag gatgtagagc ggaatgatcg tcgcctgcgg cggcaacacg
     2281 atcgtcgaga tcaggatgac aaacagaatg tttttgcccg gtagacggaa acgggcgaag
     2341 ccataggcca ccagcgtaca ggagatcaga gtgccgatgg tgccgatggt tgcgagcaac
     2401 agactattca taaacaggcg tgggaaattg acttgatccc aggcatcggt gaagttctta
     2461 aagttcgggg caaattgata aaccgcctgc caagtacgcc aacggccctt aacgttgaaa
     2521 acgccttgct ccggtgcggc tgggtcgacc atgtcactgt cctcgcggta tccttgcacc
     2581 agtgccagtt ggcgggtgcc ggtatcggtc ggtacgtcgt aaagcggata gtccgtacct
     2641 tgataattgt aagtcgctgc ggcggccggc catagcggcg cgccgatggt ggtcatttgc
     2701 gaatcgagct tgaaggccgt cgtcagcaca taggccagcg gcagcagaaa aaccaccatt
     2761 gacaacaacc ccacaccggt cacgccgagg ctgtccagtg atttgcgcat gcgcggccga
     2821 aaacgtcgag aaacgggtgt ggcgggagaa ccgccagtaa cggatgtctc tgccatcgta
     2881 gttgctcctg cctaagcctg gccgccggca taatacacca gccgctgagc agagaaaaat
     2941 agcagcccgg cggcagacat cgtcaccaca aaaatgacca cggccagaac cgaggcatac
     3001 cccatattgc tgaagacgaa tgcttcgcga tagaaaaccg tcgggaagaa gaggagcgat
     3061 ccctgtggat cgccgttcgt gccgccgatc aatagcgccg ggacaaagta ctggaaagcg
     3121 ccgatgacgc cgatggtcac attgtaaaaa atgaccggcg agatcagcgg gattgaaatg
     3181 gagaagaatt gtctcagtgg gctggcgcca tccaccgtag cggcctcgta gagttcggtc
     3241 ggcacgccct gcaagccagc cagcaggatg atcatcgcgt ttccaactgc ccagatggcc
     3301 agcaagttaa gtgctaaccc ggcccaggca ggatccgtaa accaacgcgg cgggtcggtt
     3361 atgccaatgg ccttgagcac cagatttagc cagccagact gagcgttcat cacgccatta
     3421 aaaatgattg cgcctgccac gaccgggatc acactcggca ggtaaaacag cgtccgaaag
     3481 aagctggagg ccctcaaccg ttttgaattg accagcaccg ccagatagag tggaacaatt
     3541 aagccgagag gaatgccaat cagtgcgaac tttagggtta cacccatcga tggcagcgcg
     3601 accgggtccg ttaacaggtg cttgtagtta tctaggccaa caaagttgaa gttccccagt
     3661 ttgaattccg aggagccttt atagtcggtg aaagacagga agatggtgaa taagatcggc
     3721 aacagttgaa aagcaaagaa cccgaaaatc cacggtgaca gaaatagaaa cccatgaact
     3781 ctctcccgac gggttcgagc agaaatcgag cgacggggcg cagctggcgc aaggatggtg
     3841 cgggtggtgg cagacatggc agctcctcga aatgtcctgg acgccacccg atcaggtggc
     3901 gtccaggaat acatttggcc taatcgccag ggcaaaagtt acttagcagc tttatatgca
     3961 gcgtcaagat cactcttgag tttggcgatg gcggcgtcca gatccagaag cggcgtctga
     4021 tccattaaag tgcggaatgc acctaagaga gtgttagcct gggctaagtt gggcagccaa
     4081 gcctcatgat tgggcaggtc tggatacgcg agcatcgcct gggctacggt ccagtcgatc
     4141 ttgttcggtg cggccgcctt gtctttggca gcgaagaatg ctgggcgatc agcttcaaca
     4201 gccggcatac cgccgtatat gaccggcaag tccggatccg ttaccatcgc agttaacacc
     4261 ttgaaggcca cggcttgctg cttgctttcc ttcaggatgg cgaaggtgtc gccgtggagt
     4321 ttagccgtaa tcttgccatt gatggttggc accacagcca tatcccagct cgtcttggag
     4381 ttgtcgaagc agcacatgta ccaggtgtgt acccacgaca tcgccacgtt gcccgaagcg
     4441 aaaaggttgc ccttattgaa ctgatcgctg ttctggtaat cggcggtggg catgaagtgg
     4501 tacttccaga tgccgtcgta gtaccatttc caagccgctt cccaggcgga cgggatgacg
     4561 gcagaattgg tcttcgcatc ataaggtatt ccgccgtcga agaacgcacc aagaccgcgg
     4621 gcgtccgtcc attgcgcaaa gaaaccgaac tgggtgacat tcgccggatc gaaagccgcg
     4681 ttcgtggcgt cgttgccagc cttgtcaact gtcagcttca ttgctagatc ttggaaagcc
     4741 gctaagtccc acggcttgcc attgtacagt tcaccgactt tgtgcggcgg gtaaggcagc
     4801 ttggcttcat cgaacagttt cttgttgtag aagatgaagg acgggaacag ggcaaacggc
     4861 aggcccacca acttgccctc atccttggtg aagtccaaca acttcgggtc atacttgctt
     4921 aagtcgaaac ccgcatcctt ggccaactgc gcaacgtcag cccaggcacc ctggaagctg
     4981 gcgcggccag ccttgcctgt tggccctaca atctccgggg cgttgccacc agcgatcagg
     5041 gcccgcaaag cgtcatacga gttttggcca gtattatata caacgtccat cagcacgcaa
     5101 gcttctttct gcgacttgtt gtacttgtca acccaggctt tttccaacgg cacaacatcc
     5161 gcgttggagc cagcgccgag gccgatgaac caagtgactt gggtggaagc gggatcgcaa
     5221 ccctcgccgc cagtcttaat ctccggctga atggtggggg taggaacacc agtcggtgcc
     5281 gccgtcggca cggccgtgac ctcgacttgc ttgataactt ccttggtgac gacaaccacc
     5341 accggcgtag gcgcaggtcc ggaagttgta gtaggcgcaa cagcgggcgc gcaggcactc
     5401 agtacgaaac tgaacaccat aactaaagcg aacatcaact tcatattgcg tttcatttca
     5461 tctctcctat ctttagaatt tgaatttttt tgaacctaaa cagtagacaa gcgcctaacc
     5521 attctgaata tttgctagtt ctcctccttt tagtttagtc tcgcaccgca ggaagtgcgc
     5581 acgactaact cagtcggtag agtaattcga cgtggagcgg aatggggatg ttgaatcaaa
     5641 tcgatcagcg tttcagttgc tagctcgccc gatcgatgaa tcggctgtcg aacagtggtc
     5701 aaaggtggat cggtgtgcgc ggcgaaggac atatcatcaa aaccgacgat cgccacatct
     5761 tccggcacac gcaggctagc ttcgcgcaag gcgcgcaatg caccgaccgc catggtgtca
     5821 cttgccgcaa aaacggcatc cggccggcga gcgagcaaac gctgcatcgc gctatatcca
     5881 cccgcttcgg taaaatcgcc ttcgacgatc agatctgagt caacgagctg gccccgatcg
     5941 cgtagggccg cgatatagcc gtccaagcga tcggctccag caatcatgtc atgcgggccg
     6001 gcaatcgtcc cgattcgctt acggcctaat cgcagcaaat gcaaaactgc ctcgcgtgca
     6061 ctcgcgatat tgtcgatatc gacgtagttg actcgaccat cattcggatg gcgtccattc
     6121 aagataaagg gcagatcgcc ttccagcaag gcctggacaa ccggatcgtc caacaagtta
     6181 gaagcgacaa tgacaccatc gagcaagccg ctgtgcataa tttgacggat ggtgcggcgt
     6241 tcatattcgg gttcagccag ccacagcatc actgaatgat cgtgaacatt gcacgcagcc
     6301 gaaacacctt gaatgaaact ggggaaatat ggatcggtga agagggcggc tacacccatt
     6361 ggaatgacta gacccagtac gcgggtgtag cctttggcca agccgcgcgc tgcaacgttc
     6421 ggttggaaat tcatcttgtg aatgattcca agtacccgtt cgcgagtggg cgggctgacg
     6481 tttgggtgat tgttgagcac ccgtgagaca gtggaacgag aaactttgct taatttagcg
     6541 atcgtttcaa gatccatttt ttatgggaac gctcccaaac catttgctca aaaatgccgc
     6601 ttgcacggca tttatcgaaa tattaaggga aattcggggt attcaatttg cgtccaaact
     6661 catacatatt gggaacgctc ccaatatatc attgcattaa tcagttgtca agtaccaaaa
     6721 aaattgattg ggcggccatt taacaaactg ccgtttattc aattacctcc gctatctctt
     6781 ataactttat ccccacctca ttcaatcttc acaagttttt tacaccgccc tgctatagtc
     6841 agcctgtagt taaaactgag tagttgattg catggaggtg caacatgaaa aaagtattga
     6901 tcatcttggc aaccatcgcc ggggtcattg ctttaggcaa cgtgctgatc gccggagcgc
     6961 agaacggcgt cgtgcgcttt aactcgtcga tgagttcaat gatgacgaat tacaccgcca
     7021 cttataccgg cacaatgccc tttggccgcg gcatgcatgg cgacacatat accggcacgg
     7081 ttccattcgg cggacagatg cgcgatcaaa tgtcgcagat gaatcaaaag gtattggatg
     7141 cggtgtcagc gaagttaggc atgaccacga gtgacctact ctctgctctg caaaataggc
     7201 agacgctgac tgatctcgcg aaggcgaaga acgtctcgat cagcgatctg caatcggccg
     7261 ccgatgcagc ccaccaagct gcgctcgacg atctagtcaa gcagaacgtc attacccaac
     7321 agcaagcaga tttgatgctg gcgcatatgc aagacatgcg catcttgggc tttggtggtg
     7381 gcgggcatgg ctttggcatg ccacacgaca tgccacacgg tttcggacca aacggatcat
     7441 ttccgggtcg cggcatgctg cacagttttc cgggcagcgt ccaaccgcaa cagccttccg
     7501 tgataacgcc ttcaagtcaa ggctaaagaa tttcattcaa acttgtagag acgatccgac
     7561 ggatcgtctc tacttatatt tgacgattgc ttccgaatgg tttactctag ttccatgtca
     7621 cacaccatct tagtcgtcga tgatgaactt gaaatcgtta aagtcgtgcg cgcttatttg
     7681 gagcagaacg gctttcgtgt gttgacttca gcggacggcc aacaagccct gatgatcttt
     7741 cggcacgagc agcccgatct cgttttgctc gatctgaatc tgccgtcgct cgccgggctg
     7801 gatgtctgcc gatcgatccg gcgtgaatcc aatgtgccga tcatcatgct caccgcgcgc
     7861 gtcgaagaaa ccgatcgctt gatcggcctc gaaatcggcg cagacgatta catcgtcaag
     7921 ccgttcagtc cgcgtgaagt cgtggcgcgc gtgcgcgcgg tgctgcgccg atcgatcgcc
     7981 gcgcccgaac aaccggcccg cctgtcaatc ggcgacctca agatcgatgt ggacaaacat
     8041 gaagtctatc tgcacaccca atcgatcgat ctcacgccca gcgagttcaa tattttgcat
     8101 gccatggcgg cgcagccggg ccgcgccttc tcgcgcatgg acttgctcga tgccgcgcag
     8161 ggcgaagcgt atgaaggcta cgagcgcacc atcgatgtgc atatcaaaaa tttgcggcag
     8221 aaattgggcg aagatccgcg caatcccagc ttcattctaa ccgtgtacgg catcggctat
     8281 aaatttagcg acaaagtgaa agactgacaa ggcgtccggt gaagttaagt ctcttctgga
     8341 aactcatgct ctccttcgcg ctcgtcgtgg cgatcggcct cggcgctgtc gtgctcgtcg
     8401 ctaatcaagt caccaatcgc gaactgcatg aattcatgat ggggccggat aacgacattg
     8461 gcgccgatgt gacgccgggc atgatgcttg gtcgtgttgg cccaccgcag ttgcggcaac
     8521 cggtaatcga tcgagtgaat gccgcaatca tcgtcggcag cttgctcgcg ttggcggccg
     8581 ccctcgccat cggctt
//
LOCUS       DASRTJ010000413         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_328606,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000413 DASRTJ010000000
VERSION     DASRTJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328606"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFF70_08460"
     CDS             <1..436
                     /locus_tag="VFF70_08460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44767.1"
                     /translation="GLLLGAFGLGALMGALLVGSIGARSRGRWLTLGNLAFPIALIVF
                     TVSRSFWLAFVILFAVGIVFILQNALTNTLLQVIVPDDRRGRLMSIYSLVFQGATKAG
                     GVQGGFVTSFIGAPFSVAIGAIASLFYGLFVLIRWPKIRRLE"
     gene            complement(590..1195)
                     /locus_tag="VFF70_08465"
     CDS             complement(590..1195)
                     /locus_tag="VFF70_08465"
                     /inference="COORDINATES: protein motif:HMM:NF022751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HZY44768.1"
                     /translation="MNEEIEESLIINGLRIDPVLFTEVNAKRCVLDECKGACCTGGVW
                     VDKEAVAALVPHVEAIKANLPEDRRNPNLWFSAPEPDEDFPSGISIGTNTVDDPVRPG
                     KRCCVFLRPDRFCALQVTNRQLGHEWPGLKPFYCALYPIFVGKGELSYDDETPHVYEG
                     ATCQRFCGEKQPLYKIYKEEIEMVLGVEGYQELVRMAERRK"
     gene            complement(1279..>2159)
                     /locus_tag="VFF70_08470"
     CDS             complement(1279..>2159)
                     /locus_tag="VFF70_08470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZY44769.1"
                     /translation="ISPIERLAYNEFDVRIAIRAETNTRSLTNIDPKKLALMQTARRE
                     LTEAFMRRSASGELRWVVTQFPTEASAQDADMSLREYEDFVYGACLLDDPDPILCWQQ
                     IAEQQQRYVDFLKGTKRIKIKGPNCDLTVGIAGRTFINSEGKNNFPDGEIFTGPEENV
                     TEGWVKFTYPAIYLSREVDQVELEFKQGKVVKASAGKGKDFLMSVLDTDSGSRTLGEF
                     AIGTNNAIKKFTRNILFDEKLGGTIHMALGAAYPDTGGVNQSAVHWDMICDTRDGTEI
                     TVDGKPFYRSGEFLIR"
BASE COUNT          457 a          625 c          533 g          544 t
ORIGIN      
        1 tggcctgctg ctgggcgcgt tcggtctggg cgcattgatg ggcgcgctac tcgttggatc
       61 gatcggcgct cgatcgcgcg gacgatggtt gacgttaggc aatctcgctt tcccgatcgc
      121 gctgatcgtc ttcactgtat ctcgatcgtt ctggttggct ttcgtgatct tgttcgctgt
      181 cggcatcgtc ttcatcttgc agaacgcttt aaccaatacc ttgctgcaag tgattgtgcc
      241 cgacgatcgg cgcggccgat tgatgagcat ctattcgctc gtgtttcaag gcgcgacgaa
      301 agccggcggc gtgcagggcg gcttcgtcac aagtttcatc ggcgcgccgt tctccgtcgc
      361 gatcggtgcg atcgcttcct tgttttacgg tttgtttgtg ttgattcgtt ggccaaagat
      421 tcgccgattg gagtagaaac acttcagttg aacgtctcgc gatgagtaat gcgtaacgaa
      481 tgatatatga caaggtgact tgctggcaag ctagcaaagt gaatttgtaa gcgagatcag
      541 cttgtcacct cttcaccctg tcaacctttc aagcctacaa taacgccaat tattttcgcc
      601 tttctgccat ccgcactaat tcttgataac cctccacgcc caacaccatc tcgatctctt
      661 ctttataaat cttatacaac ggctgcttct cgccgcagaa acgttggcag gttgcgcctt
      721 cataaacatg cggcgtctca tcatcgtacg acaattcgcc tttgcccaca aagatcggat
      781 agagcgcgca gtaaaacggt tttaaaccgg gccattcatg gcctaactgt cgattggtca
      841 cttgcagcgc gcagaatcga tcggggcgca ggaagacaca acagcgcttg cctggccgaa
      901 cgggatcatc gaccgtattc gtgccgatgc tgatgcctga cggaaaatct tcatcaggct
      961 ctggcgcgga gaaccacaga ttggggttgc gtcgatcttc gggcaagttc gctttgatcg
     1021 cttcgacgtg cggcacgagc gccgctaccg cctctttatc gacccacacg ccgccggtgc
     1081 agcacgcgcc tttgcattcg tccagcacgc agcgcttcgc attcacttcg gtgaacaaca
     1141 ccggatcgat ccgcaatccg ttgatgatca gcgactcttc aatttcctcg ttcatgatag
     1201 tctcctcggc taaaaataaa gggacgttct gttggaacgt ccctcaggtt acagaaaatt
     1261 gatcgggcga accaacttct aacggattaa aaactcaccg ctgcgataga acggcttgcc
     1321 atcaactgtg atctccgtac cgtcgcgcgt atcgcagatc atatcccagt gcacggcgga
     1381 ttgattcaca ccgcccgtat caggataagc cgcgcccagc gccatgtgaa tcgtcccgcc
     1441 gagtttctca tcaaataaaa tattgcgcgt gaatttcttg atcgcgttgt ttgtgccgat
     1501 cgcaaattca cccaacgtgc gcgatccgct gtccgtatcg agcacgctca ttaagaaatc
     1561 tttgccctta cccgcgctgg ccttgaccac tttaccctgc ttgaattcca attccacttg
     1621 atcgacctca cgcgataaat aaatagcggg atacgtgaac ttcacccagc cttcagtcac
     1681 attttcttcc ggcccggtaa aaatttcgcc atccgggaaa ttattcttgc cttccgaatt
     1741 gatgaacgtg cggccggcga tgccgaccgt gagatcgcaa ttaggacctt tgatcttgat
     1801 gcgcttcgtg ccttttaaga aatcaacata gcgctgctgc tgttccgcga tctgctgcca
     1861 gcacaaaatc ggatcgggat catccagcag gcacgcgccg tacacaaagt cttcatactc
     1921 gcgcaggctc atatcggcat cttgcgcgga ggcttcggtc gggaactgcg tcaccaccca
     1981 tcgcaactca ccgctcgcac tccggcgcat gaacgcctcc gtcaattcgc ggcgcgcggt
     2041 ctgcatcagc gcgagcttct tcggatcgat gttggtcagc gagcgcgtat tagtctcagc
     2101 acgaatcgcg atccgcacat cgaactcatt gtaggctagc cgctcgatcg ggctgatat
//
LOCUS       DASRTJ010000414         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_252974,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000414 DASRTJ010000000
VERSION     DASRTJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252974"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VFF70_08475"
     CDS             complement(<1..1928)
                     /locus_tag="VFF70_08475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44770.1"
                     /translation="MNQQLAVRTKFSGREWIVVAAIFLLAFALRTIDLTRVPPGLHND
                     EVVAAKLTETVVNGRLTIFFPEDTGMEPLPFYIAAPFMKLIGTTVFALRLPAIFESML
                     ALSVIFALGRRLFGKRVAIIASALFAITFWTVLFGRIVLNVTLLPPLATLLAYLFWRA
                     WSSQGKREIVLWLLSGLTLGLALLSYTASRILPAILIVFAVYVLNAHRSDWRRWWKGI
                     AITLIVGAIVSAPMFIYLAGNPSADNLNAFEIDRPLIELKNGNLQPVIETSFNTLGMF
                     AFNGDPLPYYDVPNRPVFDPITALLLAIGLLIALWRWRKAEYAFVFIWFFVSLAPGML
                     SQPAPNYTRTLGVQAVLFFLPGLAIVALIDRWHAKIIYSALAILFVGNLVWTAHDYFT
                     VWPSIDAVRFWHQSGLYAVANRLQSDLDSSPVAICLPDNLVDERTWWWNPAWRHMQYL
                     LHRPDISVRYYNCADTLILIDGSARYAFPNAIDEATLQQFPIYSQFLARSATDRLELP
                     QRLGTMLRVDRSSTPLAQVLNQISISSTLTLDVDQSSAQVPIDFGDKVQLLGYSLSKS
                     SVKPGHSFDLVTYWKVTNSLSPQLSQFTHMLNGQGDIVAQEDRLMLTSQSLQVGDVFI
                     QIHHLSMPGDLKPGEYQLS"
     gene            complement(1932..3071)
                     /gene="rffA"
                     /locus_tag="VFF70_08480"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1932..3071)
                     /gene="rffA"
                     /locus_tag="VFF70_08480"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HZY44771.1"
                     /translation="MSYRIPFNRPVFDGTEQPYIQHAMASGHMSGDGVFTKQCHELLE
                     TALGVPKVLLTTSCTHALEMSALLLDIQPGDEVIVPSFTFVSTVNAFVLRGARPVFAD
                     IRSDTLNLEETKIEALITSRTRAILVVHYAGVGCEMDAIMQIADRHHVPVIEDNAHGL
                     FGKYRGKYLGTFGVMATQSFHETKNFTCGEGGAILINDPAYIERAEIIREKGTNRSRF
                     FRGQVDQYTWVDIGSSYLPSEVLAAILYAQLQAREKIQAKRQHIWEYYDQHLQRWADD
                     HDVRRPIVPAHCEQPYHMYYLLLPSLDRRQALIAHLKERGILSVFHYLPLNLSIMGQR
                     FGGKAGDCPVTEKLSDRLLRLPFYNSLTDADLAEVVETVGSFTEM"
     gene            complement(3068..3700)
                     /locus_tag="VFF70_08485"
     CDS             complement(3068..3700)
                     /locus_tag="VFF70_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44772.1"
                     /translation="MTSSVQSLIYSHQSLYQALMHVLYGRYFDARYQAVADEIPAGAS
                     VVDLCAGNGYLYLNYLRRKNVQYLGLDISPQLVRWAQQHDVPMQRSNVWEDEIPIGDS
                     VVMQASLYQFIPQADLLLHKMLLAARDRVIVTEPIRNLSSSRNPLFSIVGRRLTVPPQ
                     NADRYHAQRFDYRSLLQLFESIASFESARLLPGGREVIGIFKGQFTQVSL"
BASE COUNT         1017 a         1004 c          982 g          765 t
ORIGIN      
        1 gacagttgat actcacctgg cttcaaatcc cccggcattg atagatgatg aatctgaatg
       61 aatacatcgc cgacttgcag actctgcgaa gtgagcatca atcgatcttc ttgcgcgacg
      121 atatcgccct gcccgttaag catatgcgtg aattgcgaca actgcggcga cagactgtta
      181 gtgactttcc aataagtcac caaatcgaac gaatggccgg gcttcacgga actcttcgac
      241 agtgaataac ccaacaattg aaccttatcc ccaaaatcga tcggcacttg tgcggatgat
      301 tgatccacat ccaaagtaag cgtggaactt attgaaattt gattcagcac ctgcgccaac
      361 ggtgtactcg atcgatcgac gcgcagcatc gtccctaacc gttgaggcag ctccagccga
      421 tcggttgcag aacgcgccag gaattggctg tagatcggaa actgttgcaa cgtcgcttca
      481 tcgatcgcat tagggaaggc atagcgcgcc gagccatcga ttaagatcag cgtgtccgcg
      541 cagttgtaat atcgaactga aatatcgggc cggtgcaaca ggtactgcat gtgccgccac
      601 gccggattcc accaccacgt ccgctcatcg accaaattat cggggagaca gatcgctacc
      661 ggcgaagaat caagatcact ttgcaatcta ttggcgaccg catacaaacc ggattgatgc
      721 cagaagcgaa ccgcatcgat cgacggccaa accgtgaagt aatcatgcgc ggtccacacc
      781 aagttgccga caaacaaaat tgccaacgcg gagtaaatta ttttggcgtg ccaacgatcg
      841 atcagcgcga cgatagcaag gccgggtaga aaaaataaaa cggcctgcac accaagcgtg
      901 cgcgtgtaat tgggcgcggg ctggctcaac atgccgggcg caagcgagac gaagaaccag
      961 atgaaaacga aagcatattc agctttgcgc caacgccaca acgcgatcaa caaaccgatc
     1021 gccagcagca atgcggtaat cggatcgaac accgggcgat tcgggacatc gtaatatggc
     1081 aacggatcac cgttgaaagc aaacatgccc aaagtattga atgaagtttc gatgaccggc
     1141 tgaaggttgc cattttttaa ttcgatcagc ggccgatcga tttcgaacgc atttagatta
     1201 tcagcgctgg gattgccagc cagataaatg aacatcggcg ccgagacaat tgcgccgacg
     1261 atgagtgtaa tggcgatccc tttccaccag cgccgccaat cggatcgatg cgcgttcagc
     1321 acgtagaccg caaagacgat gagaatcgca ggcaggatgc gtgaggcagt ataagataac
     1381 aatgccaggc cgagcgtcaa accacttaac aaccacaata caatttcacg cttgccctga
     1441 ctcgaccagg ctcgccaaaa tagatatgcc agaagagtgg ccagcggcgg cagcaaggtc
     1501 acattcaaaa cgatgcggcc aaagaggact gtccaaaacg tgatcgcaaa aagggctgag
     1561 gcaatgatcg caacgcgctt gccaaacaat ctgcggccta gcgcaaagat cactgacagg
     1621 gcgagcatac tttcgaaaat ggctggcaaa cgcaatgcaa aaaccgtggt gccgattaac
     1681 ttcataaaag gcgcggcgat ataaaacggc agtggctcca tgccggtatc ttcggggaag
     1741 aagatcgtca atcggccatt gacaacggtc tcggtcaact tggcggcaac gacttcatcg
     1801 ttgtgcaggc cgggcgggac gcgcgtgaga tcgatcgtgc gtaaagcaaa ggccagcaaa
     1861 aagatcgcag ctaccacgat ccattcacga ccagaaaatt tagttcgcac cgccagttgc
     1921 tgattcatca atcacatctc agtgaagctg cccaccgttt cgacgacttc agccagatca
     1981 gcatccgtga gtgaattgta gaaaggcaga cgcaacagac ggtcgctgag tttttcggtt
     2041 accgggcaat cgccagcctt gccgccaaac cgctgcccca taatggaaag attgagcggc
     2101 agataatgaa agacactcag aatgccgcgt tctttcaaat gcgcgatcaa ggcttgtcgt
     2161 cgatcgagcg agggcagcag cagataatac atgtgatatg gctgctcgca atgcgcaggc
     2221 acgatcggac gccggacatc atgatcatcg gcccagcgtt gaagatgttg atcgtagtat
     2281 tcccaaatat gctgacgttt ggcttgaatc ttctcgcgcg cttgaagctg cgcatataga
     2341 atcgccgcca aaacttcgga gggcagataa ctcgatccga tgtcgaccca agtgtattga
     2401 tcgacctgtc cgcgaaagaa gcggctgcga ttggtgccct tctcccgaat gatctcggcg
     2461 cgctcgatgt acgccggatc gttgatcaag attgcaccgc cttcgccgca cgtaaaattc
     2521 ttggtctcgt ggaaactctg tgtcgccata acaccgaaag ttcctaagta cttgcctcgg
     2581 tattttccaa acaagccgtg ggcgttatct tcgatcacgg gcacgtgatg ccgatcggcg
     2641 atctgcatga tcgcgtccat ctcgcagccg acgcctgcat aatgcacgac caagatcgcc
     2701 cgagtgcgcg acgtgatcaa tgcttcgatc ttggtctcct cgagattcag cgtatccgat
     2761 cggatgtcag cgaagaccgg ccgagcgccg cgcaatacaa aagcattcac cgtcgataca
     2821 aatgtaaatg aaggcacgat gacttcatcg cccggttgaa tatcgagtaa caatgccgac
     2881 atttccagcg cgtgggtgca agaggtcgtc aacagaactt tcggcacacc gagcgcggtc
     2941 tccagcaatt cgtgacattg cttggtgaag acgccatcgc ctgacatgtg cccgctggcc
     3001 atagcgtgtt gaatgtaagg ttgttccgtg ccgtcgaaaa caggtcgatt gaaaggaatg
     3061 cgatagctca taaggatacc tgtgtgaatt gacctttgaa aattccgatc acttcccggc
     3121 cgccgggcaa cagacgagcg ctctcgaatg aagcgatcga ttcaaataac tgcagaaggg
     3181 aacgatagtc aaagcgttga gcgtgatatc gatcagcatt ttggggcggc accgttaagc
     3241 gccgtcctac gatcgagaat aacggattgc gcgacgagga tagattgcga atcggctcgg
     3301 tcacgatcac tcgatcgcgt gcggccagca gcatcttgtg cagaagcaga tcggcttgcg
     3361 gaatgaactg atagaggctg gcctgcatga caacgctgtc gccgatcgga atctcgtctt
     3421 cccacacgtt cgatcgttgc atcggcacgt cgtgctgctg cgcccatcgc acgagctgcg
     3481 gtgaaatatc gaggccgagg tattgtacat tcttacgtcg cagataattc aaatacaaat
     3541 aaccattgcc ggcgcacaga tcgacgacgg acgcacccgc cggaatctca tcggccaccg
     3601 cttgatagcg cgcgtcaaag tagcgcccat acaacacatg catgagcgcc tgatacagac
     3661 tttgatgcga ataaatcaac gactgcaccg atgaagtcat atggtcctaa cacacgcgga
     3721 tgataacaca cgggctagct acgaagtaga tcctccacgg atgtgttt
//
LOCUS       DASRTJ010000415         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_124383,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000415 DASRTJ010000000
VERSION     DASRTJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124383"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFF70_08490"
     CDS             complement(<1..677)
                     /locus_tag="VFF70_08490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZY44773.1"
                     /translation="MQDPRVEKLAKVITEYSVGIKQSDRVYIMASPAAQPLTLAVIEQ
                     VIKLGGFPHITAGGNYHHLDVVPGAIESLLKYGSDEQIQYISPIERLAYNEFDVRIGI
                     RAETNTRALTNIDPKKIALLQTARRELTEAFMRRGATGELRWVVTQFPTEASAQDADM
                     SLREYEDFVYGACLLDDPDPILRWRQIAERQQRYVDFLKGTKRIKIKGPNCDLTVGIA
                     GRTFINS"
     gene            complement(747..1511)
                     /gene="pstB"
                     /locus_tag="VFF70_08495"
     CDS             complement(747..1511)
                     /gene="pstB"
                     /locus_tag="VFF70_08495"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZY44774.1"
                     /translation="MERTPKIVIENLSFSYEDGTEGLNGVNLTIYANEITALFGPAGG
                     GKSTLLRILNRLNDLVEIRKLTGRVTLDGEDIYAPSVNVIDLRRRVGIVFAQPLPLPM
                     SIRANIEYGPRLAGLHDRDKLNQIVETSLRSAAMWDEVKDRLAESGFALSGGQQQRLC
                     LARVLALEPEVILLDEPMSGLDPISTAKIEDSLQELKKQYTIVIVPHNIQQAARVSDH
                     AAFFLQGKLIEEGPGTQLFTKPREKRTEDYVTGRFG"
     gene            complement(1555..>1804)
                     /locus_tag="VFF70_08500"
     CDS             complement(1555..>1804)
                     /locus_tag="VFF70_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HZY44775.1"
                     /translation="RAAGETAPILFTVAAFFLPTLPKSVFDATMALPYHLFVISTQVP
                     NMPVQIQFGTALVLMVFVLTMNVIAAVIRSYYRRKRQW"
BASE COUNT          407 a          505 c          476 g          416 t
ORIGIN      
        1 tccgaattga tgaacgtgcg gccggcgatg ccgaccgtga gatcgcaatt aggacctttg
       61 atcttgatgc gcttcgtgcc ttttaagaaa tcaacatagc gctgctgtcg ttcagcgatc
      121 tgccgccagc gcaaaatcgg atcgggatca tccagcagac atgcgccgta cacaaaatct
      181 tcgtattcgc gcagactcat gtcggcatct tgcgcggagg cttctgtcgg gaactgcgtc
      241 accacccagc gcaactcacc ggtcgcaccc cggcgcatga acgcctccgt caattcgcgg
      301 cgcgcggtct gcagcaacgc gatctttttc ggatcgatgt tggtcagcgc gcgtgtattg
      361 gtctcggcgc gaataccgat ccgcacatcg aactcattat aagccagccg ctcgatcggg
      421 ctgatgtact gaatctgttc gtccgatccg tatttcagca gcgactcgat cgcaccgggc
      481 accacatcga gatgatgata attgccgccg gcggtgatat gcgggaagcc gcccaacttg
      541 atcacttgtt cgatcacggc cagtgtcagc ggctgcgcgg cgggggaggc catgatgtaa
      601 acccgatcac tctgcttaat tccgacggag tattcggtga taaccttggc taacttttcg
      661 acacgtgggt cttgcatgca atgtccttga gttggtgtga caacgggcgg gattatatca
      721 tcgacgccga tgaataacaa agacgcttaa ccaaagcgtc ccgtcacata gtcttcggtg
      781 cgtttttccc gcggtttggt aaacaactgc gtgcccggcc cttcttcgat tagcttgcct
      841 tgcaaaaaga aagcggcatg atcagacact cgggcggcct gctgaatatt atgcggcaca
      901 atgacgatcg tgtactgctt cttcaactct tgcagcgaat cttcgatctt cgcggtcgaa
      961 atcggatcga ggccgctcat cggctcatcc agcaaaatca cttccggttc gagcgcaagg
     1021 acgcgcgcga ggcataaacg ttgttgctgg ccgcccgata aggcgaagcc tgattcagct
     1081 aatcgatctt tgacctcgtc ccacatcgcc gccgaacgca aacttgtctc gacgatttga
     1141 ttaagcttat cccgatcgtg caggccggcc aagcgcggtc catattcaat attcgcgcgg
     1201 atcgacatgg gcagtggcag cggctgggcg aagacaatcc ccacgcggcg gcgcagatcg
     1261 atcacgttca cgcttggcgc gtaaatatct tcaccatcga gcgtcacacg gccagtcaat
     1321 tttctgatct cgactagatc attcaatcga ttcaagatac gtagcagcgt tgatttgccg
     1381 ccgcccgctg gaccaaacag cgccgtgatc tcgttagcat agatcgtcag attgacgcca
     1441 ttgagacctt cggtgccatc ttcgtaactg aaacttagat tttctataac gatcttgggt
     1501 gtgcgttcca tataattcaa tggggaaggg gcgaggtgac ctcgccctta ctaattacca
     1561 ttggcgtttg cggcgatagt aactgcgaat cactgccgcg atcacattca tcgtcaagac
     1621 gaacaccatt aagaccaacg ccgtgccgaa ttgaatttgc actggcatgt tgggcacttg
     1681 cgtgctgatg acaaacaaat gatatggcag cgccatcgtc gcatcgaaga cgctcttagg
     1741 cagtgtcggc aggaagaaag cggcaaccgt aaacaagatc ggagcggtct cccccgctgc
     1801 gcgt
//
LOCUS       DASRTJ010000416         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_124425,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000416 DASRTJ010000000
VERSION     DASRTJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124425"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFF70_08505"
     CDS             <1..813
                     /locus_tag="VFF70_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZY44776.1"
                     /translation="LQHGYRNWLATKPETMAGHAPTYYATTFPPPRSQRRGDQYGNYT
                     FDQSAPLVEGTYQAVLGSAQCAITAANLILNNQRAVYALCRPPGHHAGKDFSGGYCYF
                     NNTAIAAKMLSARGRVAILDVDYHHGNGTQDIFYDDDRVLTISIHADPSWEYPYFLGY
                     VDEIGSGKGEGFNLNIPLPKRTDGTVYFAALDRAITRIRDFDPWALVVAVGFDTFDGD
                     PISQFALKTADYTTIAQRIRSINLPTVVVQEGGYNTEALGRNAVAFLNPFVE"
     gene            complement(895..1791)
                     /gene="folD"
                     /locus_tag="VFF70_08510"
     CDS             complement(895..1791)
                     /gene="folD"
                     /locus_tag="VFF70_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZY44777.1"
                     /translation="MTAKLIDGKAVAATIHAEVRQAAVELKTRSGFAPGLATVLVGDD
                     PASHSYVKAKRQACAEDGIDSFPHELPATTSEVDVIALVKQLGEDSKVHGILVQLPMP
                     KHINEEKVLNTVPITKDVDGFHPINIGKLGMKNREPSFAPCTPAGVIELLDRIGTPIE
                     GANAVVLGRSNIVGLPVSLLLLGRNATVTVCHSRTKDIGAITRQADILIAAIGKPRFV
                     KADMVKPGAVVIDVGVNRVEDATKKSGYRLVGDCDFDAVSEVAAAITPVPGGVGPLTI
                     AMLMKNTLRAAETFAKEMLVTA"
     gene            1988..2602
                     /locus_tag="VFF70_08515"
     CDS             1988..2602
                     /locus_tag="VFF70_08515"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZY44778.1"
                     /translation="MTAMSQLTELAAEVSACTQCILHKSRVKAVPGEGPENASIMFIG
                     EGPGFNENQQGRPFIGAAGHFLDELLHSIGLKRDQVYITNVVKCRPPGNRDPLPEEIE
                     ACRQYLDRQIALIKPKVIVTLGRFSMARAFPNVKISQVHGQPRKVDGIVYVPMFHPAA
                     ALHQPSLRKTVEEDFQKLPKILAELEKLAENDKPFEDAQQLSMF"
     gene            2623..>2816
                     /locus_tag="VFF70_08520"
     CDS             2623..>2816
                     /locus_tag="VFF70_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44779.1"
                     /translation="MSDRKIYRWRRSIATLMLAAIGISVLVVNMPAARSISAHSTNLS
                     PAVGSSYLYRFDPISQTWFT"
BASE COUNT          653 a          742 c          775 g          646 t
ORIGIN      
        1 cttcagcacg gctatcgcaa ttggctggca acgaagccag aaacgatggc aggtcatgcg
       61 ccgacttatt acgccaccac ttttccgccg cctcgatcac aacggcgcgg cgatcaatat
      121 ggcaactaca cgttcgatca aagtgcgccg ttggttgagg gaacctatca agcggtgttg
      181 ggctcggcgc agtgtgcgat cacagccgcg aatttgattt tgaacaacca gcgcgcagta
      241 tatgcgttgt gccgcccacc gggccatcac gcaggcaaag atttcagcgg cggatattgt
      301 tacttcaaca acaccgccat cgccgccaag atgttgagcg cgcgcggccg cgtggcgatc
      361 cttgatgtcg attatcatca tggcaacggc acgcaggata ttttctacga cgatgatcgc
      421 gtgctgacga tttcgattca cgccgatccg agttgggagt atccgtattt tctcggctac
      481 gttgatgaga ttggttccgg taaaggcgaa ggcttcaact tgaatattcc tctgccgaag
      541 cgaacagatg gcacggttta tttcgcggcg ctcgatcgcg cgatcacgcg aattcgtgat
      601 ttcgacccgt gggcattagt cgtcgccgtc gggtttgata cgttcgatgg tgatccgatc
      661 agccagtttg ccttgaagac cgctgactac acgacgatcg cgcagcgcat ccgatcgatc
      721 aacttaccga cggtggttgt tcaagaaggt ggttacaaca cagaggcgtt aggcaggaat
      781 gcggtggcgt tcttgaaccc gtttgttgaa tgagcgatgg agtgaatgag ggaaagagag
      841 aaggagagat gaaataatcg ctcaatcttt ccttccctct accgctctat caaatcaggc
      901 tgtaacaagc atttccttcg caaacgtctc cgccgcgcgc agcgtattct tcatcaacat
      961 ggcgatcgtc aatgggccca cgccgccagg cacaggcgtg atcgccgcag cgacttcgct
     1021 gaccgcatcg aaatcgcagt caccgaccaa tcgatagccg gatttcttgg tggcatcttc
     1081 aactcgattg acacccacat cgatcacgac ggcgccgggc ttgaccatat cggcctttac
     1141 aaagcgcggc ttgccaatgg cggcgataag aatatcggcc tggcgtgtga tcgcgccgat
     1201 gtctttggtg cgcgaatggc agacggtgac cgtcgcattg cggccaagca gcaacaatga
     1261 aaccggcaga cccacaatgt tgctacgacc caataccacg gcattcgcac cttcgatcgg
     1321 tgtgccgatt cgatcgagca attcgatcac accggccggt gtgcagggcg cgaaagacgg
     1381 ttcgcggttc ttcatgccga gcttgccgat gttgatcgga tgaaagccat cgacatcttt
     1441 ggtgatcggc acggtgttca gcaccttctc ttcattaata tgcttgggca taggcagctg
     1501 caccaaaatg ccatgcactt tggaatcttc acccaactgc ttgaccaacg caatcacatc
     1561 cacttccgaa gtggtggccg gcagttcatg tggaaatgaa tcgatgccat cctccgcgca
     1621 ggcctggcgt ttggctttca cgtacgaatg cgaagcggga tcgtcaccga cgagaaccgt
     1681 ggccaagccg ggcgcgaaac ccgatcgggt tttaagctca accgccgctt gccgcacttc
     1741 ggcatgaatc gttgctgcaa ctgcttttcc gtcgatcaat ttagctgtca tggttgctcc
     1801 atagaaaatg atgataagat agcttaaagt ttagcgttgg gccgccggga agtgcagtgg
     1861 caaatgtcat cttcaaacaa gaaagttgac atgcttactg gtgtacggta gacaaaacac
     1921 attgcatcgt ctataatcgc ccaacattgt atgacaagcc tgttcgaacg cgtaaatttg
     1981 ttatgggatg acggccatgt cgcaactgac tgaactcgcc gctgaagttt cagcctgcac
     2041 tcaatgtatt ttacacaagt cgcgcgtgaa agcagtgccg ggcgaagggc cggagaacgc
     2101 gtcgatcatg ttcattggtg aggggccggg ctttaatgaa aatcagcagg gccgaccatt
     2161 tatcggcgcg gccggacatt ttttggatga gctgctgcac tcgatcgggt tgaagcgcga
     2221 tcaggtttat attaccaacg tggtgaagtg ccgtccgccc ggcaatcgcg atccgctgcc
     2281 ggaagagatc gaagcgtgcc ggcagtatct cgatcggcag atcgcgttga ttaagcccaa
     2341 agtcattgtc acgctgggtc gcttctcgat ggcgcgcgcg tttccgaatg tgaagatcag
     2401 ccaagtgcat ggtcagccgc gcaaagtcga tggcattgtg tatgtgccga tgtttcatcc
     2461 agcggcggcg ctgcatcagc cgagtctacg caaaaccgtt gaagaagatt ttcagaagct
     2521 gccgaagatt ctggcagagc ttgaaaaatt agcggagaac gataaaccct ttgaggacgc
     2581 tcaacaactg agcatgttct gatgaatcga tcggaaggga ctatgagcga tcgaaaaatt
     2641 taccgttggc gtcgatcgat cgcgacgttg atgctggccg caatcggaat cagcgtgctt
     2701 gtcgtgaata tgccagccgc tcgatcgatc tcggcgcact cgacgaatct ttcaccggca
     2761 gtcggatcga gttacctgta tcgcttcgat cccatttcac agacgtggtt cacgta
//
LOCUS       DASRTJ010000417         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_253174,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000417 DASRTJ010000000
VERSION     DASRTJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253174"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1382)
                     /locus_tag="VFF70_08525"
     CDS             complement(273..1382)
                     /locus_tag="VFF70_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HZY44780.1"
                     /translation="MDQSQIASIRNACTRFVSGHYPLTVPQMLNELIAAAQPDLRSDI
                     YGQGDFINDFEKRIAELLGKEAAVFMPSGTMAQPIALRIWSDRNHSQHVAFHPTCHLE
                     LHEQKGYQLLHGLHGVLVGSPAQLMTLADLDRVNEPLAALLIELPQREIGGQLPEWND
                     LVAMTHWARERGIALHLDGARLWECQPFYQREYREIAALFDTVYVSFYKILGGIAGAI
                     LAGPADIIAEARIWQRRQGGNLIRLFPYVLSAQIGLEQRLPRMTEYRKKAVEIAQALT
                     PFLQIDVVPNPPQVNMLHVFLRSPKDRLEAAALEIAQEQKVWLFNQPLTPTEIPELNK
                     FELTVGDAALDLDTNEIAALFRSVFEQADRLERAD"
BASE COUNT          373 a          408 c          440 g          356 t
ORIGIN      
        1 gaaaaacttt ttagaaactc tgcattctct gtgtctcggc ggtgaaaaca aaaacccgac
       61 tgcgaagatc atagtcgggc gtgagagagc acaaggtctg gtgcggtctg cgtctcggtc
      121 gcgctgcgat ccaagcgata gcacaagttg gttgtttagt tgtgtagtct aattgtcact
      181 cgctgctcag gtaagtcagc ggcaattgaa ttataccccg ctctacaatc acgtgcaata
      241 cgagttgaat ctaaattgta aaagagccga ctttagtcgg ctctttctaa tcgatcggct
      301 tgttcgaaaa ccgatcgaaa cagcgcggcg atctcattcg tatccagatc gagcgcggcg
      361 tcgccgacgg ttaactcgaa cttgttcagt tccggtattt cagtcggcgt caacggttga
      421 ttgaacagcc acactttttg ctcttgtgca atttcaagcg ccgccgcttc caatcgatct
      481 ttcgggctgc gcaggaagac atgcagcata ttgacttgcg gcggattcgg cactacatcg
      541 atctgcagga agggagtcaa ggcttgcgcg atctcaacag ccttcttgcg atactctgtc
      601 atgcgtggta agcgctgctc caatccgatc tgagctgaca acacatacgg aaacaaacgg
      661 attaaatttc cgccttgccg acgctgccaa atgcgcgcct cggcgatgat gtcggccggg
      721 ccagccaaaa ttgcgccggc gatgccgccc agtattttgt agaaagacac atagaccgta
      781 tcaaacaacg ccgcaatctc gcgatactcg cgctgataaa acggctgaca ttcccacaag
      841 cgcgcgccat ccaggtgcag cgcgatgccg cgttcgcgcg cccagtgcgt catggcgacg
      901 agatcattcc attcaggcaa ctgtccgccg atctcgcgct gcggcagttc gatcaacaac
      961 gcggcgagcg gttcgttaac ccgatcgaga tcggccagcg tcatgagctg cgcgggcgat
     1021 ccaaccaata cgccgtgcag gccatgcagc agttgataac ctttttgctc gtgcaattca
     1081 aggtggcaag ttggatgaaa agctacatgc tggctgtgat tgcgatccga ccagatacgc
     1141 aaagcaatgg gctgtgccat tgtgccgctg ggcatgaaga ccgcagcctc tttgcctaac
     1201 agctcggcga tgcgtttttc aaagtcgttg atgaaatcgc cctgaccgta gatgtctgat
     1261 cgaagatcgg gctgggctgc ggcgatcaat tcgttcaaca tctgtggcac ggtaagagga
     1321 tagtgcccgc tcacaaaacg tgtgcaggca tttcgaatcg atgcgatttg agattgatcc
     1381 atgcggccgc ctatgatccg atcgggttca gcagatggcc tgcattatag catcgaccgg
     1441 aagagagggg cggcgcgagc gatgtgtttc gttcgatcga tttgtggctg aaagcgagca
     1501 ggcgaggggc aaggagcgag agatgaaagc atcctgagcg aaatggtgca tctgaaggat
     1561 tcagcatgca gtcgaag
//
LOCUS       DASRTJ010000418         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_25932,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000418 DASRTJ010000000
VERSION     DASRTJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25932"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFF70_08530"
     CDS             complement(<1..609)
                     /locus_tag="VFF70_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZY44781.1"
                     /translation="MTTLKLPGLIDPHVHLREPGATHKEDYGSGTSAALAGGFTCVLD
                     MPNNTPPITDAITLAAKQQAARSHARCDYGIHLGAGNDNVETAASLANQVVGLKMYLD
                     QTFGSLRLDDLDSLMKHMERWPHDRPLMCHAEGRTVAACILVAHVMHRPVHICHVSLA
                     DEIDLIRRAKDKGIDVTCEVTPHHFFLSNDDAAAIGSGRSEVR"
     gene            complement(936..1937)
                     /locus_tag="VFF70_08535"
     CDS             complement(936..1937)
                     /locus_tag="VFF70_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZY44782.1"
                     /translation="MKLNNNITDVRGIEVGHAHTLRGITGCTVILCTGGAVGGVDQRG
                     GAPGTRETDALHPMHLVNVVHAIVLAGGSAFGLEAASGAVKFLEEKRIGFDVGVARVP
                     IVPAAILFDLGLGQSNVRPDAKMGYQACLNAKRGKMIEGNVGAGMGATVGKILGIKHA
                     MKSGLGSASMSIGKEVIVAAIVAVNAFGDVIDPETSQIVAGARSAQPKRSRKLFIPYF
                     ADTMSIMKRSTLPGFARRSRSNTVIGVVATNAKFNKEQINTVAQMAQDGIARTIRPAH
                     TMVDGDTLFALATGEKKADVNVVGAFAAEVVAQAILNGVRAARSIAGLPAWIDRPSP"
     gene            complement(1981..3147)
                     /locus_tag="VFF70_08540"
     CDS             complement(1981..3147)
                     /locus_tag="VFF70_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZY44783.1"
                     /translation="MEFDPNIAHQYATSIARPRRVGSGENEKVAAEITDRLRSFGYQV
                     DCEPFAFSIAPQLFLALEILLGLMLIAAMFAPDNRARIIPSILFIAFGLAANPINRAV
                     RSRSVLSPAHQPRPLGKWHTTLSSTNIVATLPDLSTDSPLPQIYLVAHYDSKSQWLPL
                     ALRIILFVLAITCGLIFATLNLFGADALFANPFGFIAFISALPLLLLDVGNRSPGAID
                     NASGVGLVLHLAECLRAVPVDRPYNLTVLIPNAEEFSLMGSAAYVKLHARQLGREASR
                     VHVLNFDGIGVDGDLRWIGRGSKLSDLIQQTCRDLNLKLSRFNIHGALYDHLSFADHG
                     LDAVSLIAIGPASRSIHTLQDSIDKLHVRGFDQAGRVALKVIENLTTDYTAVAD"
     gene            complement(3300..3812)
                     /locus_tag="VFF70_08545"
     CDS             complement(3300..3812)
                     /locus_tag="VFF70_08545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZY44784.1"
                     /translation="MAKQYSAAPAMVIDPKKHYTATFKTDVGEFVVELYADKVPKTVN
                     NFVFLARDHFYDGVTFHRVIRDFMVQGGDPTGTGSGGPGYKFADEFHPSLRHTGPGIL
                     SMANAGPNTNGSQFFITHDATPHLDNKHTVFGKVIKGMDIVLAIPDRDPGRARTPGAK
                     ITAIEITEME"
     gene            complement(3875..4399)
                     /locus_tag="VFF70_08550"
     CDS             complement(3875..4399)
                     /locus_tag="VFF70_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZY44785.1"
                     /translation="MSIEPKQDVQTVSFDGEPIQRMIHGVVIRPAVTQIDDRGTLCEI
                     LRPDWDFHPAPLTYVYQFTIRPGMIKGWHVHHQHDDRIFISQGTVKVVLYDDRPDSPT
                     YKTINVIVRSDLQRSIMIIPSHVYHAHQNIGSTDALMISMPTRLYDHESPDVYRYPID
                     NDYIPYQFIDVRGY"
BASE COUNT         1064 a         1197 c         1172 g         1037 t
ORIGIN      
        1 ccgcacttcc gagcgacccg atccgatcgc cgccgcatca tcattcgaca aaaagaaatg
       61 atgcggcgtg acttcgcagg tgacatcgat gcccttgtct ttcgcccggc ggatcagatc
      121 gatctcatcc gcaaggctga cgtgacaaat gtggacgggg cgatgcatga catgggcaac
      181 taggatgcac gccgcaaccg tgcggccttc ggcatgacac atcaacggcc gatcatgcgg
      241 ccagcgctcc atatgcttca ttaacgaatc gagatcgtcc aagcgcagcg aaccgaatgt
      301 ttgatcgaga tacatcttca agccgacaac ttgattcgcc agcgaagcgg cggtttcaac
      361 attatcgttt cccgcgccga gatgaatacc gtaatcgcag cgcgcgtgcg atcgagccgc
      421 ttgctgcttg gccgcgagcg tgatcgcatc ggtgatcggc ggcgtgttgt tgggcatatc
      481 gagcacgcag gtgaatccac ccgccaacgc ggcagatgtt ccgctgccat agtcttcttt
      541 atgcgtcgcg cccggttcac gcaaatgcac atgcggatcg atcaagcccg gtagttttaa
      601 tgtagtcata tttaaaacct tttaccactg agacacggag gcgcacagtt ttatttcgtt
      661 tttctccgtg tctcaatgtt tctgtagttt aatcaattca taaaaaaaga gccgacgaaa
      721 tgtcggctct tcaaaaatat gaaacactca atcgtgcggc caagaatttc ttcggcccgc
      781 tgaggcggtg cattattatc ggtgtgaatt tcaatcgaat cttcatgatc agcaaatcgc
      841 gcgcgatctt actcgcgttg agggaatgtg tcaagaggca atctacatga ttcatccggc
      901 ctttcaactc cgaccctctc ccttttcgag gaggcttagg gtgagggtcg atcaatccat
      961 gcgggcagac ccgcgatcga tcgtgcggca cgcacaccat tcaagatcgc ttgtgccacc
     1021 acttctgccg cgaacgcgcc gacaacattc acgtcggctt tcttttcgcc cgtcgccagc
     1081 gcaaacaaag tgtcgccatc gaccatcgtg tgcgcgggtc gaatcgtgcg tgcgatcccg
     1141 tcttgcgcca tctgcgccac tgtgttgatc tgttccttgt tgaatttcgc gttcgtggcc
     1201 acgacgccga tcaccgtatt cgatctcgat cgacgcgcga agccaggtag cgtcgatcgt
     1261 ttcatgatcg acatcgtatc agcgaagtat ggaataaaga gctttctcga tcgttttggt
     1321 tgcgccgatc gagcaccggc cacgatctga cttgtttcag gatcgatcac atcaccaaac
     1381 gcattcacgg caacgatcgc ggcgacgatc acttccttcc cgatcgacat actggccgat
     1441 ccgaggccgg acttcatcgc gtgtttgatg ccgaggattt ttccgacggt tgcgcccatg
     1501 cccgcgccga cattgccttc tatcattttt ccgcgcttcg cattcaggca ggcttgataa
     1561 cccatcttcg catcaggccg cacgttcgat tgaccaaggc cgagatcgaa caagatcgca
     1621 gcaggcacga tcggcacgcg agccacaccc acatcgaagc caatcctctt ttcttcaaga
     1681 aatttcactg cgcccgacgc ggcctccaac ccaaaagccg atccgcctgc tagtacaatc
     1741 gcgtgcacga cattaacgag atgcatcgga tggagcgcat cggtctcgcg cgtgcccggt
     1801 gcgccgccac gttgatcgac gccaccgact gcgccgcctg tacacaagat cactgtgcaa
     1861 cctgtaatcc ctcgaagggt gtgcgcatgt cccacttcga tgccgcgtac atcggtgatg
     1921 ttgttgttta gtttcatcgt accctcacat tcgcgcagat gcgtacaggt tggggagttt
     1981 ttaatcggcg acagccgtgt aatccgtggt taaattttca atcactttca acgccacccg
     2041 ccccgcttga tcgaagccgc gcacgtgcaa tttgtcgatt gagtcttgca gcgtgtggat
     2101 cgatcggcta gccggaccga tcgctatcaa ggatactgcg tcgaggccgt gatcggcaaa
     2161 cgacaggtga tcgtacaacg cgccgtgtat attgaatcga ctgagtttca aattcagatc
     2221 gcggcaagtt tgttgaatca gatcagataa cttactaccc cgtccgatcc aacggagatc
     2281 accgtcgacg ccgataccat caaagtttag cacatgcacg cggctcgctt cacgaccgag
     2341 ttgtctggca tgcagcttca cgtaagcggc cgaacccatt aatgaaaact cttcggcatt
     2401 ggggattaaa accgtcaaat tataggggcg gtctactggg acggcgcgga gacattcagc
     2461 caaatgtaaa acaaggccaa cgcccgacgc gttatcaatt gctccgggcg atcgattgcc
     2521 gacatcgagc agaagcagtg gtagggcgga gatgaatgca ataaaaccga atggattagc
     2581 gaaaagagcg tctgcgccaa agaggttgag tgtcgcaaag atgaggccac acgttatagc
     2641 gagaacaaag agtattatgc gcagggcaag tggcagccac tggctttttg aatcatagtg
     2701 tgcgacgaga taaatctgcg gcagtgggga atctgttgac agatcaggca gcgttgcgac
     2761 aatattggtg ctgcttaaag tcgtatgcca ttttccaagc ggccgaggct gatgtgcggg
     2821 cgagagcact gatcgagatc gcactgcgcg attgatcgga tttgcggcca gcccgaaggc
     2881 tatgaacaag atcgatggaa taatacgagc gcgattgtcc ggtgcgaaca tcgctgcgat
     2941 caacatcaaa cccaaaagga tttcaagcgc gagaaagagt tgaggcgcga tcgagaaagc
     3001 aaacggctca caatcgactt gatagccgaa tgatcgcaat cgatctgtga tctcagcggc
     3061 cactttctcg ttttcgccag aaccgacacg ccgtgggcgc gcgatcgagg tggcatactg
     3121 atgcgcgatg tttggatcga attccatatg atcggcagcg ctccttcgaa ctaaaacaaa
     3181 aagggcaaac acatgtccgc cctttactat tttcagtcac cctgagcgca gcgaaggctc
     3241 gcacacaatt agccgaggtt cttcgagcgc taacgcgctc tcagaatgac gcgcacgatc
     3301 tattccattt cagtaatttc aatcgcggta atcttcgcgc cgggtgttcg tgctcgaccg
     3361 ggatcgcgat cgggaatggc aagcacaatg tccatgcctt tgatcacttt gccaaacacc
     3421 gtgtgtttat tatcgagatg cggcgtggcg tcatgcgtga taaagaattg actgccgttg
     3481 gtatttggac cggcattcgc catcgacaag atgcctgggc cggtatggcg caatgacgga
     3541 tggaactcgt cggcgaattt ataacccggg ccgcctgagc cggtgcccgt cggatcgccg
     3601 ccctgtacca tgaaatcgcg gatgacgcga tggaacgtca caccgtcata aaaatggtcg
     3661 cgcgccagga agacaaagtt gttgacggtc ttcggcactt tgtcggcgta tagctcgacg
     3721 acgaattcgc cgacatcagt tttgaaggtc gccgtgtaat gcttctttgg atcgatcacc
     3781 attgcgggtg ctgcagaata ctgtttagcc atgaatctcc tattaagtgt cagttgcctg
     3841 tattgtacct gcgattttat tttgatggta tcggctaata accgcgtaca tcgataaact
     3901 gatacggaat gtaatcgtta tcgatcgggt aacggtacac atcgggcgat tcgtgatcat
     3961 acagacgcgt gggcatgctg atcatcaacg catcggtgct gccgatattt tgatgcgcgt
     4021 ggtaaacgtg agacggtatg atcatgatcg agcgctgcaa gtccgatcga acgatcacgt
     4081 tgatggtctt ataggttggt gagtcaggcc gatcgtcata cagcacgact ttgaccgtgc
     4141 cttgactgat aaagattcga tcgtcatgct gatgatgcac gtgccagccc ttgatcatac
     4201 ccggtcgaat tgtgaactga tagacgtagg tcagcggcgc aggatgaaaa tcccaatcag
     4261 ggcgcaggat ttcgcataac gtcccgcgat cgtcgatctg cgtcacagcg ggacggatca
     4321 caacaccgtg aatcatccgc tgaataggtt caccatcgaa cgataccgtt tgaacatctt
     4381 gttttggctc tatgctcata cttaacctcc agagaaaatt gtagtctcag cgacaaataa
     4441 tacgccacaa acaaaagagg gcagagtcct
//
LOCUS       DASRTJ010000419         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_25997,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000419 DASRTJ010000000
VERSION     DASRTJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25997"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFF70_08555"
     CDS             <1..1017
                     /locus_tag="VFF70_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZY44786.1"
                     /translation="RLGFRAITPVFDGADEEEIEAELARAWLIDAAWNDITAQAWVWL
                     KGQTGEDQSDELDDDDEVRRLYLADWLAGKEYDEDLLATDDTYIRRTILREWLREKGF
                     DPDYLLMFEDDGRGHGVRKQADLDVIDACLRLWLQAHDVEMMKAPSAEVMLRAQQVSA
                     EMGEPIPTLGKMPLYDGKTGDRFDQPVTVGIITMMKLAHLVEDKVHARSTGPYSLVTQ
                     QPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTVKSDDVQGRVKTYESIVKGEKI
                     EEPGIPASFRVLVKEMQSLGLAVEAITDTGEVMRFGKDEERQRVPKLSMGLLGSLAGD
                     DDNA"
     gene            1207..1638
                     /gene="rpsL"
                     /locus_tag="VFF70_08560"
     CDS             1207..1638
                     /gene="rpsL"
                     /locus_tag="VFF70_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZY44787.1"
                     /translation="MPTINQLIRKGRKVKRRKGKSPALQFTWNSFAQRRMRRAKGAPQ
                     KRGVCTIVRTQTPKKPNSALRKVARVRLSNGIEVSAYIPGEGHNLQEHSVVLVRGGKV
                     KDLPGVRYHIVRGSLDTAGVEKRSQGRSKYGAKRPKVKPAA"
BASE COUNT          440 a          410 c          576 g          399 t
ORIGIN      
        1 cggttaggct tccgtgcgat tacgccggtc ttcgacgggg cagatgaaga agagatcgag
       61 gctgaactcg cgcgggcttg gttgatcgat gcggcttgga atgacattac ggcgcaagcg
      121 tgggtgtggc taaaagggca aaccggcgaa gatcaatcgg acgagctgga tgatgatgat
      181 gaagttcgac gattgtattt ggccgattgg ctggccggca aagagtatga tgaagatttg
      241 ttggcgaccg atgacaccta tatccgccgg acgattttgc gcgaatggtt gcgtgagaag
      301 ggtttcgatc cggattactt gctcatgttc gaagatgatg gccgtgggca tggtgtgcgt
      361 aaacaagccg acctcgatgt catcgatgcc tgtttgcgat tatggctgca agcacatgac
      421 gtagagatga tgaaggctcc gagtgcggaa gtgatgttgc gcgcgcagca agtcagtgca
      481 gagatgggtg aaccgattcc aacgcttggc aaaatgccat tgtatgatgg caagacgggc
      541 gatcgattcg atcagcctgt cacggtgggc attatcacga tgatgaaact cgcccacttg
      601 gttgaggata aagtgcacgc ccgatcgact ggtccatact ccctggttac tcagcagccg
      661 ttgggcggca aagcacagtt cggtggtcag cgcttcggcg aaatggaagt gtgggccctc
      721 gaagcttatg gcgcggcaca tactttgcaa gaaatgctca ccgttaagtc ggatgatgtg
      781 caaggccgtg tcaagactta cgaatcgatc gtgaaaggcg agaagatcga agagccaggg
      841 attcctgcat cgttcagagt cttagtgaaa gagatgcagt cgctgggctt ggcagtcgaa
      901 gcgatcacgg atacgggtga agtgatgcgt tttggaaaag atgaagagcg tcaacgggta
      961 ccgaaactaa gcatgggcct gctgggtagc ctcgccggcg atgatgataa cgcttaatcg
     1021 acgtcatcac gccgaaattt tctgatcgcg gggttgacag actccgtgca gtatggtact
     1081 atcgaacgcc gctgggcaaa atggcgcggc ttactcccgt tgccgaacaa ttaggaacga
     1141 tttgttttcc gaggaaaggc catcgtgctt tcgaggtggt cttttttcac ggaggttgaa
     1201 gttcaagtgc cgacgatcaa tcaactgatt cgcaaaggcc gcaaggtaaa gcgccgcaag
     1261 ggcaagtcgc ctgcattgca gttcacgtgg aactcatttg cgcagcgacg gatgcgccgc
     1321 gcaaaaggtg cgccgcagaa acgcggtgtg tgcacgatcg tgcggacgca aacccccaag
     1381 aaaccgaact cggcgctgcg caaagttgcg cgtgtgcgtt tatcgaatgg cattgaagtc
     1441 tcggcgtaca tacccggcga aggccataat ctgcaggagc attcggtggt gttagtgcga
     1501 ggcggtaagg tgaaggactt gccgggtgta cgctatcaca ttgtgcgcgg cagcttggat
     1561 acggctggcg ttgaaaagcg ttcacaaggt cgatcgaagt atggtgccaa gcggcctaaa
     1621 gtaaaacccg cggcttagga aatcaggaga aggaagcata tgtcacgacg ggctcgtgcg
     1681 gttaagcggc ctcttgcgcc ggatttacgt tacaacagta ttctggttca gcaattcatc
     1741 caacggatga tgcgtggcgg aaagaagagt accgcgcagc gtgtcctcta tggatcgttt
     1801 gctttaattg aagaacgcgc caagc
//
LOCUS       DASRTJ010000420         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_64060,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000420 DASRTJ010000000
VERSION     DASRTJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64060"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFF70_08565"
     CDS             complement(<1..442)
                     /locus_tag="VFF70_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZY44788.1"
                     /translation="MDYRDYYQILGVTRGATQDEIKKAYRKLARQYHPDNNPGDKKSE
                     AKFKEINEANEVLSDPEKRKKYNQLGSQWQQYQRAGGAPGGFDWGQWTSTQGQSARGQ
                     ADLNDVFGGGDFSDFFTSIFGGAGAASRSRRRGPTRGQDLEQPIE"
     gene            complement(637..1632)
                     /locus_tag="VFF70_08570"
     CDS             complement(637..1632)
                     /locus_tag="VFF70_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY44789.1"
                     /translation="MQKRKLGNSGLEVSAIGLGCMGMNFSYAPFPDKQEMISLIRTAV
                     ERGVTFFDTAEVYGPFTNEELVGEALALFRGQIVIATKFGFKPASSGEARWSELDSRP
                     EHIKEAAEASLKRLKVDAIDLFYQHRVDPNVPIEDVAGAVKDLIQAGKVKHFGLSEAG
                     VQTIRRAHAVQQVTALQSEYSLWMRTPENEVIPALEELGIGFVPFSPLGKGFLTGKID
                     ENTNFDSSDFRNTIPRFTPEARKANQALIDLLSKVSKRKNATPAQIALAWLLAQKPWI
                     VPIPGTTKLHRLDENIGAVAIQLTPDDLLEIENAASKITVQGARYPETLEKLTGR"
     gene            2060..>2445
                     /locus_tag="VFF70_08575"
     CDS             2060..>2445
                     /locus_tag="VFF70_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZY44790.1"
                     /translation="MTRQTITIDGNEAAAYVAYRVSEVIAIYPITPSSAMGEWADAWS
                     AVDTKNIWGTRPLVQEMQSEGGAAGAVHGALQTGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARSIAAQALSIFGDHA"
BASE COUNT          534 a          672 c          639 g          600 t
ORIGIN      
        1 tctcgatcgg ttgttccagg tcttggccgc gcgtgggacc gcgccgacgc gatcggctgg
       61 ccgcacctgc cccaccaaaa atgctcgtga agaaatcgga aaaatcgccg ccgccaaaaa
      121 catcgttgag atcggcctgg ccgcgagcgc tctggccttg agtgctggtc cactggcccc
      181 aatcgaaacc gcccggagcg ccgccggcgc gttgatattg ctgccactgc gatcccagtt
      241 gattatattt cttgcgcttc tcgggatcgc tcaagacttc attagcttca ttaatttctt
      301 taaatttcgc ctcagatttt ttatcgccgg gattgttgtc gggatgatat tggcgcgcca
      361 gcttgcgata cgcttttttg atttcgtcct gcgtagcgcc gcgcgtcacg ccgaggattt
      421 ggtaatagtc tctgtaatcc atatgaattc acactgctca ctgtttaatg catacttatc
      481 tgcttgtatt atacctgaca cgaaatgact gcggcgggga cgcggcaagg ggaaaacatg
      541 cgggcgatcg aaatttcgat cgcccgcatg tgctttggtt agccagcaat tgtcaattac
      601 ctttacgggc ggctaaacga tctcgccatc agccgatcag cggccagtca atttctccag
      661 cgtttcgggg tagcgagccc cctgcaccgt gattttcgat gcggcgtttt caatctcaag
      721 taaatcatcg ggggtcaatt ggatcgctac cgccccgatg ttctcgtcta gccgatgcag
      781 cttcgttgtg cccggaatcg gaacaatcca cggcttttgg gcaagcagcc aggcaagcgc
      841 gatctgagca ggtgtcgcat tcttccgctt tgaaacctta ctgagcaggt caatcagggc
      901 ttggttcgct ttccgcgcct ccggcgtaaa gcgcgggatg gtgttacgga aatcgctgct
      961 atcgaagttc gtattttcgt cgatcttgcc tgtcagaaaa ccttttccca gagggctgaa
     1021 tggaacaaac ccgatgccga gctcttccag ggccggtatc acttcattct caggagtcct
     1081 catccacagc gagtattcgc tctggagtgc agtgacctgc tgaacggcat gtgcgcgccg
     1141 gatggtttgc acgcctgctt cagagagacc aaaatgcttg accttgcctg cctggatcag
     1201 atcctttacc gcccctgcta cgtcttcgat gggcacgttc ggatccactc ggtgttgata
     1261 gaacaaatcg atggcatcaa ccttaagccg cttgagcgag gcctcagcag cttctttgat
     1321 gtgctccgga cgactatcca gttcactcca cctcgcctct ccgctggaag ccggcttgaa
     1381 cccgaacttg gtggcgatca cgatttgccc acgaaagagc gccagggctt cgccaacaag
     1441 ttcttcattt gtaaacgggc catacacttc agcagtgtca aaaaatgtga cgccacgttc
     1501 gacggccgta cgaatcagcg agatcatctc ctgcttgtct ggaaacggag cgtaggagaa
     1561 gttcattccc atacagccga gcccgatagc ggaaacttcc aagccgctgt ttcctagttt
     1621 acgcttttgc attttcaaat tcctgtcttt gagtgaatag gtattacaga ggtgatgtcc
     1681 aaagatgcgg gtcaactact gcttaaagtt aaaaattccg tataacattc ctgtgaggag
     1741 tattagacgg attgatcctc aacaatagta taagccaaac aattaccgcg tcaagttttt
     1801 aggccttcaa acgttgtcgc ccacgctggt tgccgcgaca cttgcaccgc aatgcggagc
     1861 agtgcaggtg tttcaatcgc tgccgtttgg catttatgca caattctgca accccaccta
     1921 taaatctgcc gatttaagac gatgatcaac ccaaatcaat gcctaacgtc actgctaaca
     1981 tttgcgcgat cgtgcgatag tttattgtac gatatttctc tccctgactc atgaggccag
     2041 cgagcaatag aggcgaatga tgacacgaca gacgattacg atcgatggta acgaagcggc
     2101 ggcttatgtg gcctaccgcg tcagcgaggt gattgccatc tacccgatta cgccctccag
     2161 cgccatgggc gaatgggccg atgcctggtc ggcggtagac acgaagaaca tctggggcac
     2221 gcggcccttg gtgcaggaaa tgcaaagcga aggcggcgcg gcgggcgcgg tgcatggcgc
     2281 gttgcagact ggctcgttga cgacgacctt caccgcgtca caaggtctgt tgttgatgat
     2341 tcccaacatg tacaagatcg caggcgagtt gacctccact gtctttcacg tcgcggcgcg
     2401 atcgatcgcg gcgcaggcac tgtcgatctt cggcgatcac gcgga
//
LOCUS       DASRTJ010000421         6816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_329134,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000421 DASRTJ010000000
VERSION     DASRTJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329134"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFF70_08580"
     CDS             <1..350
                     /locus_tag="VFF70_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZY44791.1"
                     /translation="NLIDRVMVSSFNPFSLRKVKSIEPRIVCGLLYSPDMPIYLRRAW
                     LAPIIPHLDAHHPHHQKVTASNVRRLHTQGKKINVWTANDIDAIRSAIEFGVDGIMGD
                     DPVFIREILPSNK"
     gene            385..1239
                     /locus_tag="VFF70_08585"
     CDS             385..1239
                     /locus_tag="VFF70_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HZY44792.1"
                     /translation="MTKKLIINADDFGRTRGVSAGIIRAHLEGIVTSTTAMMNMPGVA
                     NDLQKAKAEAPNLGLGVHLTFTAGRPLLPTEWVTSLIDEHGKFLAQEAIVAEPTRIKI
                     EELKSELTSQIRTFKNAVGQMPDHLDAHHFVHIIPQLLTAYLDLAAEFKLPVRIPIPR
                     NETALAAFDLPPHLMGDLSPAAIETLIRADWELMEIHSIRSTDQCLLNFYGDNISVAS
                     FLKILDGLPEGITEVMAHPGLVDEQLQKESTYTLQRETEILILSDPRVKDRIRECGIE
                     LITFGSLP"
     gene            1349..2014
                     /locus_tag="VFF70_08590"
     CDS             1349..2014
                     /locus_tag="VFF70_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY44793.1"
                     /translation="MSLRRKFLRFVFHLLYNQFAFTYDLVASLVSFGQWKNWGRTSLS
                     RVHGDRILELGHGPGHLLIALARSGRTPIGIDLSPHMSRIARSNLRRAKLNVPHVRGR
                     SQALPFRSNSFEASVATFPTEYIVDPKTLSEVRRVTNDRGRLVIVAGAQIGTRKPSAR
                     LIHWLYRVTGQKDALPNGSESVFTQVNMPAHTETELIGHSQVLLVIAEKISPDNKLAP
                     SSD"
     gene            complement(1977..2465)
                     /locus_tag="VFF70_08595"
     CDS             complement(1977..2465)
                     /locus_tag="VFF70_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44794.1"
                     /translation="MSDRPRFDAHILTPTLQVAGQAEPIGPWLDFLNNRDKHTFTVHA
                     ARIMSIGVAAAPVDRPQLFMNRDQVSMIALLGLSARDGITMVRNSVMAIIHIGPIVCR
                     GEIHLSVDTPLAIYFDDLIGNYFPVTNADLHALSPLPAPLPAKADIVLINRSMVQAYY
                     PG"
     gene            2528..4090
                     /gene="hutH"
                     /locus_tag="VFF70_08600"
     CDS             2528..4090
                     /gene="hutH"
                     /locus_tag="VFF70_08600"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZY44795.1"
                     /translation="MATEKLSIDGETLSIENTLEVARRNRSVELASTARARVNASRAW
                     VDQVAATGQPTVYGINTGFGIFSTKHIPADQADRLSRNLIISHSIGVGEPFDEEIVRA
                     AMLIRANTLAKGFSGIRSETLDTLIAMLNAGVHPIVPSQGSLGASGDLAPLSHLMLML
                     SRDPDDDAIDSGNAIYHGERMSGYAAMQRAGIKRRVLNAKEALALNNGATFSAAIAAL
                     AVIDAENLLENSIISLAMSLEALRGVSRAYDAHLHQARRQLGQIEIAKRVRALIAGST
                     LIDSTDHVQDAYSLRAAPQVLGAVKDSIAHVRSIIERELNAATDNPLIFLDLESDNKA
                     LSGGNFHGEPIALVMDFLSIAASEIASISERRIARLVDDKLSNGLPSMLAENGGLDSG
                     WMMTQYTAASLVSENKTLAHPDSVDSIPTSANQEDFNPMATSAARHARSIIRNGEYVI
                     ALELMAAAQAIDLRMHADPKIKLGLATAKAHARIRREVKYLDRDRLIAPDAAKIVEMV
                     RSGDVIVSIDSN"
     gene            4405..5676
                     /gene="hisS"
                     /locus_tag="VFF70_08605"
     CDS             4405..5676
                     /gene="hisS"
                     /locus_tag="VFF70_08605"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZY44796.1"
                     /translation="MKTTIRPIKGTRDFYPEDMAFRTWLYDQMKAASQKFGYQEYEAP
                     ILEPLELYAAKSGEELVKEQSFVFTDRGGDEITLRPELTPSLARMVAQKQRELIKPIR
                     WWSFGPFWRYERPQKGRTREFFQWNIDLIGANSAYADAELVAVLVEFFRSIGLSSKQV
                     VIKVNNRKLMEEQIKAIGFQADQIDGVYKLIDKLDKLPADKWAVYGEETLGLNKNQID
                     QLRGLVNDRGLWQKSSELTEFFNAIKDFGISEYVDYDSAIIRGLAYYTGVVFEAKELQ
                     GNNRSICGGGRYDNLVGDVGGEPMGGIGFAMGDVVIGLVLEEYSLKPNLRPAPSEVLV
                     TVFDTSLMSTSAKIATLIRSAGLKAELYPEVAKLDKQLKYANAQAIPLALVIGPDEAS
                     KNQVTVKDLRSGQQQTIEQAQITATIRSLLK"
     gene            5711..5782
                     /locus_tag="VFF70_08610"
     tRNA            5711..5782
                     /locus_tag="VFF70_08610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5743..5745,aa:His,seq:gtg)
     gene            5850..5922
                     /locus_tag="VFF70_08615"
     tRNA            5850..5922
                     /locus_tag="VFF70_08615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5883..5885,aa:Arg,seq:tcg)
     gene            complement(5999..6385)
                     /locus_tag="VFF70_08620"
     CDS             complement(5999..6385)
                     /locus_tag="VFF70_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44797.1"
                     /translation="MIQPSIMLVDDDENILTLYRLALRRKPDWKLCSAADGEHALQLV
                     DECTPDFVVLDVMLPGIDGIEICRRLRLQASTAKVPVVMLSARDDAAVHRAARAAGAD
                     DFWLKPISPADFVNRIDRWLNGPPIA"
BASE COUNT         1714 a         1807 c         1762 g         1533 t
ORIGIN      
        1 ataatctcat cgatcgagtc atggtgtcat ccttcaatcc gttcagccta cgcaaagtaa
       61 aatcgatcga gccgcgcatc gtctgcgggc tgctctactc gcccgatatg ccgatctatt
      121 tgcggcgcgc gtggctcgcg ccgatcattc cacatcttga tgcgcatcat ccgcaccacc
      181 agaaagtcac agcaagcaac gtgaggcgcc tgcatacgca aggtaagaaa atcaacgtct
      241 ggacagcgaa tgatatcgac gcgattcgat ccgcaattga attcggtgtc gatggtatta
      301 tgggcgacga tccggtgttc attcgagaga tattaccgag caacaagtaa tgagtaaata
      361 tattcattac caaaggaaac aatcttgacc aaaaaactga ttatcaacgc cgacgatttt
      421 ggccgcacgc gcggtgtcag cgcgggaatt attcgcgccc accttgaggg catcgttacc
      481 agcactaccg cgatgatgaa tatgcccgga gtggcgaacg atttacagaa agccaaagcc
      541 gaagcgccga atttgggtct cggcgtgcat ctcacattta cggccgggcg gccgctcctg
      601 ccgaccgagt gggtaacctc gttgattgac gagcatggaa agttcctcgc acaagaggcg
      661 atcgttgccg agccaacccg catcaagatc gaggaattaa aatcggaact cacgtcgcaa
      721 atcagaactt tcaagaacgc cgtcggccaa atgccggatc atttggacgc gcatcacttc
      781 gtgcatatca tcccgcagct actcacggcc tatctcgatc tggccgctga gttcaaattg
      841 cccgttcgaa ttccaatccc gcgcaatgag accgcgcttg ctgcattcga tttaccgcca
      901 cacttgatgg gcgatctatc gccagcagcg atcgagactt tgatcagagc cgattgggaa
      961 ttgatggaaa ttcactccat ccgatcgacc gatcagtgct tattgaattt ctacggcgat
     1021 aatatctctg tggcaagctt tctgaaaatt ctagacggcc tgcccgaagg tatcactgaa
     1081 gtgatggctc atcccggttt ggtcgatgaa cagctgcaaa aagaatctac ctacactctt
     1141 caacgtgaaa ccgaaatttt gattctgagc gatccgcggg tcaaagatcg catccgcgaa
     1201 tgcggcattg aattgattac cttcggatct ttgccgtagg agtgacgcat ttgcacacac
     1261 gcatttatca atcgacatag cagccacaaa ttggatcgcc atcgaccaga atccacttac
     1321 gaatacgtcg cccctacatt tgataatcat gtcgcttcgt cggaaattcc tgcgcttcgt
     1381 ctttcactta ctctataatc aattcgcctt cacctatgat ctcgtcgcgt cgctggtctc
     1441 tttcggtcaa tggaaaaatt ggggtcgcac ctcactcagc cgagtgcacg gcgatcgaat
     1501 tctggagttg ggccatggcc ccggtcatct attgatcgcc ctcgcccgat caggccgaac
     1561 accgatcggg atcgatctat cgccgcacat gagccgcatc gctcgcagca acttgcgccg
     1621 cgcaaagttg aatgtgccgc acgtgcgcgg tcgatcgcaa gcgctgcctt ttagatcaaa
     1681 tagttttgag gcaagtgtgg ccacatttcc gactgaatat attgttgatc ccaaaacgtt
     1741 gagcgaggtc aggcgtgtga cgaatgatcg agggcgatta gtcatcgtcg cgggtgcgca
     1801 gatcggcact cggaaaccga gcgcgcgctt aattcattgg ctgtatcgcg tcaccgggca
     1861 aaaagacgcg ctgcccaatg gatcagaatc agtcttcact caagtcaaca tgccggcgca
     1921 cactgaaacg gaattgatcg ggcacagcca ggtgctgctg gtcatcgccg aaaaaatcag
     1981 cccggataat aagcttgcac catcgagcga ttgatcaaca cgatatccgc ttttgccggc
     2041 agcggcgcag gcagcgggct caaggcatgc aaatcagcat tcgtcaccgg aaaataattc
     2101 ccgatgaggt catcaaaata aatggcgagc ggtgtatcga cgctcaaatg aatttcgcca
     2161 cggcagacga tcgggccaat gtgaataatt gccatgaccg aattgcgcac catcgtaatg
     2221 ccgtcacgcg ctgacaatcc cagcagtgca atcatactca cttgatcgcg attcataaac
     2281 agctgcggcc gatcgaccgg cgctgccgct accccaatcg acatgatgcg cgcagcgtgt
     2341 accgtgaaag tgtgtttgtc gcggttgttt aaaaaatcga gccacgggcc aatcggctcg
     2401 gcctggccgg caacttgcag cgtgggtgtt aaaatatgtg catcaaaacg cgggcggtct
     2461 gacacgggtt tacctcctga gcaagatacg gctattctac tctattcgat tctaagagca
     2521 aatgatcatg gcgacagaaa aattaagcat tgatggcgaa actttgtcga tcgaaaacac
     2581 gctcgaggtt gcccgccgaa accgatcggt tgagctcgcc tcaacggcgc gtgcgcgcgt
     2641 aaatgcatca cgcgcgtggg tcgatcaagt cgcagccact ggacaaccaa ccgtctatgg
     2701 catcaacacc ggcttcggaa ttttttcaac gaagcacatt cccgccgatc aagccgatcg
     2761 cttgagccgc aacttgatca tcagtcactc gatcggcgtg ggcgagccgt tcgatgaaga
     2821 gatcgtccgc gccgcgatgt tgatccgcgc gaatacatta gctaaaggct tttccggcat
     2881 ccgatccgaa acactcgaca cgctgattgc catgctcaac gcaggcgtgc atccgatcgt
     2941 cccatcgcaa ggatcattgg gcgcgagcgg cgatctcgcg ccgttgtcac atttgatgct
     3001 catgttgtca cgcgatcccg atgatgatgc catagatagc ggtaatgcaa tctatcacgg
     3061 cgagcgaatg tccggctatg ccgccatgca gcgcgcaggc attaagcgca gagtgctaaa
     3121 tgcaaaagag gcactggctc tcaataacgg cgcaacgttc tcagcggcaa ttgctgccct
     3181 ggccgtgatc gatgcggaga atctgctgga gaactcgatc atatctttgg caatgtcgct
     3241 cgaagccttg cgcggcgtct cgcgtgccta cgacgctcat ttgcatcaag cgcgacggca
     3301 acttggccag atcgagatcg ctaagcgagt tcgagcattg atcgcgggca gcaccttgat
     3361 cgattcgacc gatcacgtgc aagatgcata ctcgctgcgc gccgcgccgc aagtattggg
     3421 cgcagtcaaa gattcgatcg cccacgtccg atcgatcatc gagcgcgaac tgaatgccgc
     3481 aacggacaat ccattgatct tcctcgatct tgaaagcgac aacaaagcct taagcggcgg
     3541 caacttccac ggcgaaccga tcgcactcgt gatggacttc ctatcgatcg ccgcttcaga
     3601 gattgcatcc atcagtgaac gccgcatcgc gcgacttgtg gacgacaaac ttagcaacgg
     3661 actgccctcg atgctagctg agaacggcgg cctcgatagc ggctggatga tgacgcaata
     3721 cacagccgct tcactcgtgt ctgaaaacaa aacgctggct catccagact cggttgattc
     3781 gatcccgacc tcagccaatc aagaagactt caatccgatg gcgacttcgg ccgcgcgtca
     3841 tgcacgatcg atcattcgca acggcgaata tgtcatcgcg ctggagctga tggccgccgc
     3901 gcaggcgatc gatctaagaa tgcacgccga tccgaagatc aagttaggcc tagccacagc
     3961 caaagcgcat gccagaattc ggcgagaagt gaagtatctc gatcgtgatc gattgatcgc
     4021 gccggatgcg gcgaagatcg ttgagatggt gagaagtggt gatgtcatag tatcaatcga
     4081 ctctaattaa ctgcagagag tagagtgcgc ggttctttat atttattctt tgcgatctcc
     4141 acgcgcaggg gggtgaaagc tgactcattg acacacctct tactcattgg tataattccg
     4201 ccatcatgaa cacacagata gatctgactc gacagcacca tactcatacg caccagcagc
     4261 ctgtgcgctg tttctgtttg ggtcacgact gagtgaatgc agaccgaagt cgatgaggcg
     4321 tgatccacaa ggtcacgctt ttttgtttgc cccaccctat ccctccccgc ttcgcgggag
     4381 aggggatgag tgaagaggtt gatcatgaaa acaacaattc gccctattaa gggcacacgc
     4441 gatttttatc cggaagacat ggctttccgc acatggctgt acgatcaaat gaaagccgca
     4501 tctcagaaat ttggctatca agaatacgaa gcaccgatct tggaaccgct cgaactgtac
     4561 gcggccaaga gcggcgaaga gttggtgaaa gagcaatcgt tcgtgttcac cgatcgcggc
     4621 ggcgatgaga ttacactgcg gccggagctc acgcccagcc ttgcccgcat ggtggcgcag
     4681 aaacaacgcg agttgatcaa acccatccgc tggtggtcgt tcgggccgtt ctggcgctac
     4741 gagcgtccgc agaaaggccg cacgcgtgaa ttcttccaat ggaacatcga tctcatcggt
     4801 gcaaattctg cctatgccga tgcggaactg gttgccgtgt tggtggaatt ctttcgatcg
     4861 attggcttat cgagcaagca agtcgtcatc aaagtcaaca atcgcaagtt gatggaagag
     4921 cagatcaagg cgattggttt ccaagccgat cagatcgatg gcgtctacaa gttaatcgac
     4981 aaactcgaca agctgcccgc tgacaagtgg gctgtttatg gcgaagaaac tttgggcttg
     5041 aataagaacc agatcgatca gctgcgcggc ttagtgaatg atcgtggctt gtggcagaag
     5101 tcgagtgaac ttacagaatt ctttaacgcg atcaaagatt ttggtatcag cgagtatgtg
     5161 gattatgatt cagcaatcat tcgcggcttg gcctactata ccggggttgt gttcgaagcg
     5221 aaggaactcc agggcaacaa tcgatcgatc tgcggcggcg gccgttacga taatctcgtc
     5281 ggtgatgtcg gcggcgaacc aatgggcgga atcggttttg cgatggggga tgtggtgatc
     5341 ggtttagtgc tggaagagta ttcgcttaaa ccgaatttgc gaccggcccc aagcgaagtc
     5401 ctggttacgg tttttgacac ctcgttaatg agcacatcgg ccaagattgc cacgctgatt
     5461 cgatcagctg gactgaaagc cgaactttat ccagaagtag ctaaactcga caagcagttg
     5521 aagtatgcga atgcgcaagc gatcccattg gcgctggtga tcggcccgga tgaagccagc
     5581 aagaatcaag taacggtgaa agatcttcga tcgggtcagc aacaaacgat cgagcaggct
     5641 caaatcaccg cgactattcg atcgctgttg aaatagtcaa tcgcccgtgg tacaatgcca
     5701 gcgtaatatg gtgcctgtag ctcaatggca gagcactgga ctgtggatcc agatgttgcg
     5761 ggttcgagac ccgtcaggca cccctgaaca atgacaaatg atcaaataac aaatttgtca
     5821 tttgatcatt tttcattagt tatttaaacg ccctcgtagc tcaatggata gagcgcctga
     5881 cttcggatca gacggttgtg ggttcgactc ccgccgaggg cattgaaata ttcaaatgaa
     5941 acgtccgcac tgtaatatta cagcatggac gttttttgta atgagtctca gatcgatctc
     6001 aagcgatcgg cggaccattc agccagcgat cgattcgatt gacgaaatcg gccggtgaaa
     6061 tgggcttgag ccaaaaatcg tcggcacctg ccgcccgtgc agctcgatgc acggcggcat
     6121 catcccgcgc cgataacatc accaccggca ctttagccgt cgatgcctgc agacgcaggc
     6181 ggcggcagat ctcaatccca tcgatgcccg gcagcatcac atccaacacc acaaaatccg
     6241 gagtacattc atcgaccaat tgcagggcat gttcaccatc ggccgcgctg cataatttcc
     6301 agtccggctt gcgccttaag gccaaacggt aaagtgttaa gatgttctca tcatcatcga
     6361 ccagcatgat actgggttga atcatagcaa acctctgggc ggcaaagctt aaacactaat
     6421 agcctgttgt caggcaattg ttgctaatcc attttcattc tctgctgaat tgtccgattg
     6481 gtcgcccagc tctaatgtgt tagcggccaa cctcaagctt gatcaggaga atgtcgatac
     6541 gggttaagat acaatcaaga tgagcgcggc gtacaatttg gatcacacat caggttcaag
     6601 acgaaactcg aaagtggttc aagtgcaatc aagaattgat ccaatgagga tcacaacatg
     6661 atgcgcgctg taagtcttat cgggatcggg cagaacccga tcggcacgtc caatacagtg
     6721 ccagtgcgag gattgccgat ctgatcaatc tattcgactg ctcgcctgtg cgcgcatttg
     6781 tttttcaagt gatatacccc agcgctctgt tgatat
//
LOCUS       DASRTJ010000422         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_329138,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000422 DASRTJ010000000
VERSION     DASRTJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329138"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..613)
                     /locus_tag="VFF70_08625"
     CDS             complement(23..613)
                     /locus_tag="VFF70_08625"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZY44798.1"
                     /translation="MRLTRSFFTQSTRQLAQSLIGCRLVRRWKDQRLAGVIVETEAYV
                     SEKDLACHARAGRTARTEVMYRQPGIAYVYFTYGMHWMLNIVSERVDFPAAVLIRAIE
                     PFEGIAAMQQRRGSRPITDLCSGPAKLTQALWIDQDLNGVDLCDRTSDLWLEPDRSIS
                     PQSIQRGPRIGLGATPEPWLSKPWRFWLKENPFVSG"
     gene            759..1556
                     /locus_tag="VFF70_08630"
     CDS             759..1556
                     /locus_tag="VFF70_08630"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY44799.1"
                     /translation="MTDHIVAKRWSIVISIGLALWIAARPVTTQAQTASPIETPTLSI
                     ASPLPTVVPTQPATAAPTLAATDIPLPTFTPLPTLTPLPTMTPPPTLAPAFTATPIPL
                     ASNLGTTLLTLLTDPNNLPIVIGGAAALLLLIMLLIVLARRRRQHRHIQSTEPISPVA
                     VPTPAATLEFTDAAGKPIVIPLDKKALVVGRGAGSDIELPDSLPNIDTVSRQHVRISR
                     DQDGYNIHDLESENGLKVNGRHTNYNLLEDGDRISFGSVEATFHYKR"
     gene            1670..3493
                     /locus_tag="VFF70_08635"
     CDS             1670..3493
                     /locus_tag="VFF70_08635"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZY44800.1"
                     /translation="MKCTNCGTQNPDDAKDCRRCGAVLPLAADVTQPALASDLNRTQP
                     LTNEAEATATRLRNDPRGTPRPLLKTNDKDGTRPLPRIKMFFEPLPERAIIANDQYKI
                     GSLIDETPMLNTYTAISRRAMVECKNCGWTRNQFGDQDCKNCGTSLANLEPHYPLFRV
                     KETIAPDAIAPERKIAEMNLQHAGVLLPIEAFNETVFGTRRSYVVLPEPSPFTGTQLP
                     APPESTDVVNWGLQLADALAFLHKHNIALGAADLEHISIGGHTARWFNFGAAHVNDGS
                     SRGAKQYTEDVVNLTAGLFTLLTGRSYSPKVPVSPPGLSDTFQQVFNGKITTAVQLAD
                     RLRDAIQEIRRPNSYDLRVGRLSDVGQMRQINEDSLLTLEAGLIHRSISSPIGLYVVA
                     DGMGGQAAGDAASGLAVDTLAQQAAESLMLPTLNTTPTQVDIDAWLRTAIAQANTAVY
                     QQRAAADNNMGTTLVMAVVTDGEAHVAHLGDSRAYLINQAGIKQITIDHSLVQRLIDT
                     KQLSLEEAKTYPQRNVIYKNLGDRPTVEPDIQRVPLQSGDRLLLCSDGLSGYVDDQKI
                     HQLVVAAHSPQEACRKLIDAANANSGPDNITAIIIQLETLQ"
     gene            3490..4023
                     /locus_tag="VFF70_08640"
     CDS             3490..4023
                     /locus_tag="VFF70_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY44801.1"
                     /translation="MTAGPLGASTVQRRRWVCSVCSTFNEPGARFCDHCGTPLPNSIG
                     SICPNCRTLNLLGDSFCEQCGTALTHSPYLIITDSGQHVNLFHGDEDSIVIGRSDPLS
                     GVAPDIDLNPFGAEAAGVSRRHIRLTRRDDQIWLEDLNSINLTYINNQRLTPDRPQAL
                     NDGDLLHLGRLLLTFRA"
     gene            4209..>4444
                     /locus_tag="VFF70_08645"
     CDS             4209..>4444
                     /locus_tag="VFF70_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44802.1"
                     /translation="MTPTRPGVLHVRTKTDAVKEYHYNDAKPGKPLYLLIPGGLIALA
                     AIGLIIYIAYQTWFNHELETSQGLPLILLLAPFY"
BASE COUNT         1028 a         1338 c         1158 g          920 t
ORIGIN      
        1 caacacgcat cccgcagaag tcttatcccg aaacaaacgg attttccttc aaccagaacc
       61 gccacggttt gctcaaccac ggttcgggcg ttgcgcctaa accaatgcgc ggaccgcgct
      121 ggatcgattg gggtgagatc gaacgatcgg gttccagcca gagatcgctg gttcgatcgc
      181 acagatcaac accgttgagg tcctgatcga tccacagggc ttgcgtcaac ttcgccgggc
      241 cgctgcagag atcggtgatt ggccgcgagc cgcgccgctg ctgcatcgcc gcgatccctt
      301 cgaacggttc gatggcgcga atgagcaccg ccgccggaaa atcgacgcgc tcactgacga
      361 tgttcaacat ccaatgcatg ccgtaagtga aataaacata cgcgataccg ggttggcgat
      421 acatcacttc ggtgcgagct gtgcgaccgg cgcgcgcgtg acaggctaaa tctttttcgc
      481 tgacgtaagc ttcagtttcg acaatgacgc cggccaagcg ttgatccttc caacgccgga
      541 ctaagcggca accgatcaaa gattgagcga gttgtcttgt agactgtgtg aagaacgatc
      601 gggtcagacg catgtgacac tacgcgccac gcaacatgct ttattcatcc ttcatccctc
      661 agccttggtc cttgagattg cgagtttacc ttagtcaatg ccatttggca acaccttgcg
      721 gcgcggttat aatacagccc acgaccgagg agcgaaggat gacagatcat attgtggcga
      781 aacgctggag tatagtgatt tcgatcggac tggcgctgtg gatcgcggcc agaccggtca
      841 ccacgcaggc gcaaacagct agcccgatcg aaacgccaac cttatcgatc gcatcaccac
      901 ttccaacggt tgttcccact cagcccgcga cagctgctcc aaccttagcg gctaccgata
      961 ttccattgcc gaccttcacg ccactgccca ctttgacacc gctgcctact atgacgccgc
     1021 cgccgacact ggcacctgcc ttcacagcaa cacctatacc gctcgccagc aatttaggaa
     1081 ccaccttgct caccttattg accgatccca ataacctgcc gatcgtcatc ggtggtgcgg
     1141 ccgcgctgct gctgctgatc atgctgttga tcgtcttagc ccgccgccga cgccaacacc
     1201 ggcacatcca atcgaccgag ccgatcagcc cggtagccgt tccaacgccg gcagccactt
     1261 tggaatttac ggatgcggcg ggtaaaccga tcgtgatccc gttggacaaa aaagcactgg
     1321 tcgtggggcg gggcgctggc agcgatattg agctgcccga ctcgctgccg aatatcgata
     1381 ccgtctcacg ccagcacgta cgcatcagcc gcgatcagga cggttataac atacatgacc
     1441 tcgaatcgga gaacggtctc aaagtgaacg gtaggcacac gaactacaat ttattggaag
     1501 acggtgatag aatctcattc ggttcggtcg aagcaacttt ccactataag aggtagaaat
     1561 taaccgcacg gccacgaagc tacaaagtat ttcatcattc tttgagccgc gtgacttgct
     1621 ggttaattgg tttacgagta aagatgcgtc tatcaatgag ggattgctca tgaagtgcac
     1681 caactgcggc actcaaaatc cagatgatgc caaggactgt cgacgctgcg gagccgtgct
     1741 gccactggcc gccgatgtca ctcaaccggc cctcgccagc gatctcaatc gaacgcagcc
     1801 cttgaccaac gaagctgaag ccaccgccac gcgcctgcgg aacgatccgc gcggcacgcc
     1861 gcggcctcta ctgaaaacaa acgacaaaga cggcacccgc ccgctgcccc gcatcaaaat
     1921 gttttttgag ccgctgcccg aacgcgccat catcgctaat gatcaatata aaattggcag
     1981 cctgattgac gaaacgccga tgctgaatac ctacacagcc atcagccgcc gcgcgatggt
     2041 cgaatgcaag aactgcggct ggacgcgcaa tcagtttggc gatcaagact gcaagaactg
     2101 cggcacgagt ttggctaacc ttgaaccgca ctatccgttg ttccgcgtca aggagacgat
     2161 tgcgccggat gccatcgcac cagagcgcaa gatcgctgag atgaatttgc agcatgccgg
     2221 tgtcctgctg ccaatcgaag cgttcaacga aacggttttc ggcacacgcc gtagctacgt
     2281 cgtgctgccg gagccatcgc catttaccgg cacgcagctg cccgcgccgc ccgaatcaac
     2341 ggatgtcgtc aattggggtt tgcagttagc cgatgcgctg gcgttcctgc acaagcacaa
     2401 cattgcattg ggtgcagccg atctggaaca catctcaatc ggcgggcaca ccgcgcgctg
     2461 gtttaacttt ggcgcggcgc acgtcaatga cggcagttcg cgcggtgcga aacagtacac
     2521 ggaggatgtt gtcaatctga ccgccggttt gttcacgctg ctgaccggtc gatcgtattc
     2581 gcccaaagtg ccggtctcgc cgccaggttt gtctgatact tttcaacaag tattcaacgg
     2641 caaaataacc acggcggtac agttagccga tcggctgcgc gacgcgattc aggaaatccg
     2701 gcggcctaac agttacgatc tgcgcgtcgg ccgattgagc gatgtcggtc agatgcgtca
     2761 gatcaatgaa gacagtctgc ttactttaga ggccggcctg atccaccgat cgatcagctc
     2821 accgatcggg ttatacgtcg tcgccgatgg catgggcggt caagcggccg gtgatgccgc
     2881 cagcggtctg gccgtggata cgttggcgca acaagcggct gaaagtttga tgctgcccac
     2941 actcaacacc acgccaactc aagtcgatat tgatgcgtgg ctgcggactg cgattgcgca
     3001 agccaacacg gcggtctatc aacagcgcgc cgccgccgac aacaacatgg gcaccacgtt
     3061 ggtcatggcg gtcgtaacgg atggcgaggc gcatgtggca catctgggcg acagccgcgc
     3121 ctatttgatc aatcaggcgg gcatcaaaca gatcacgatc gatcattcat tggtccagcg
     3181 cctgatcgat actaagcagc tctctctgga agaagccaaa acatatccgc agcgcaacgt
     3241 gatctacaag aatcttggcg atcgacctac cgttgaaccg gatatccagc gcgttccgct
     3301 gcaatccggc gatcgattgt tattgtgttc agacggtctc agtggctacg tggacgatca
     3361 aaagattcac cagcttgttg tcgccgcaca ttcaccgcag gaagcctgcc gcaaattgat
     3421 cgacgcggca aatgccaaca gcgggccgga taacatcacg gccatcatca ttcagttgga
     3481 aactctgcaa tgaccgccgg gccgctcggc gcttcgactg ttcaacgccg ccgatgggta
     3541 tgctcggtct gcagcacctt caacgaaccc ggcgcgcgct tctgcgatca ctgcggcact
     3601 cctctaccca actcgatcgg ttcgatctgc cccaactgcc gcacgctgaa cctgctcggt
     3661 gattcgttct gtgagcaatg cggcacggct ttaacccata gcccttattt gatcatcacc
     3721 gacagcggcc agcatgtaaa tttatttcac ggcgatgaag actcaatcgt catcggccga
     3781 tcggatccgc tcagcggcgt cgcgcctgat atcgatctca acccgttcgg cgctgaagct
     3841 gctggcgtgt cacggcggca tatacgcctc actcgccgtg acgatcaaat ttggctcgaa
     3901 gatctgaaca gcatcaatct cacctacatc aacaatcaac gcctgacgcc cgatcgacca
     3961 caggcgttga acgacggcga cttgctgcat cttggtcgcc tgttgttgac gtttagagcg
     4021 tgacgagtga tgcgtagcgc ctagtgcctg atttttttct aaagctaata aatcttcaac
     4081 gtccttgcga gccgcgctgt ggcaaagcaa tctcaaattc gatcaggaga ttgtttcgtc
     4141 ggcctaacgg cctcctcgca atgacgacgg aagatgcaaa tccagtaaca acaaaggaga
     4201 ctgcaactat gacccccact cgacccggcg tcctccatgt ccgcaccaag accgacgcgg
     4261 tcaaagagta tcactataat gatgccaaac ccggcaagcc gctataccta ttgatccccg
     4321 gcggcttgat cgcgctcgcc gcaatcggtt tgattatcta catcgcttat cagacgtggt
     4381 tcaatcatga gctggaaacc agccagggct tgccgctgat cttactgctc gcgccattct
     4441 atat
//
LOCUS       DASRTJ010000423         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_276386,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000423 DASRTJ010000000
VERSION     DASRTJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276386"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFF70_08650"
     CDS             <1..1206
                     /locus_tag="VFF70_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY44803.1"
                     /translation="STYDRLFPLFFGSNQPPMLDGTGGMSSRDQEMLADALRQMLKEL
                     RAELLRRLAEGRSLTPEELEQLGQNAGLQNANQRYQQQWITRRMLQQLGLNDLAEAID
                     ELMELLAALGMDAQSRQELRNSMDVNGEAIEQQVKDYVGQQIAENMAKEYEKREPLDD
                     LMHRPFASLTEEEADELRTQVQRLAAQLRSRAALRQKRGKDGTLDPKATVRANLKYGS
                     VPIEMKFRHKQLKPKLVLIADVSTSMRPVAEFMVRLMYELSDQIARARSFAFINHIHE
                     VSQDFDHGRASEVVQDVLEKIPPGYYNTDLGSALADFTHDHFDAVDHRTTVIFLGDGR
                     NNFNNPRLDLIDQIKRRAKRVLWFNPEHPLQWRSGDSDMLQYAPLCDSVHQVSNLAEL
                     AAAVDRLFD"
     gene            1413..>2448
                     /locus_tag="VFF70_08655"
     CDS             1413..>2448
                     /locus_tag="VFF70_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44804.1"
                     /translation="MTIHYNSNLESTTRRLTTVLFIGQGLALLAIFTTSAISSITGTM
                     LGGTARVAGWPSAATLIGGSLAAYPIGRLMGHVGRRIGLSIGYVIGILGGIVAGLGVI
                     AQSLPVFLIGSMLLGAARVSSDQARYAAADVSTLRSRARAVSLIVFAGTIGAVFGPAL
                     VPIADRAAQAIGLSQLVGPWLISGVLFVIPLIVINIFLRPDPRDIAQRLSTANDGGQE
                     TLILAPARSLLQVLAHPAARVALIALALAQIVMVMVMTITPVHMLDYAHPLEDISLVI
                     SAHIVGMYGLSMVTGWLSDRVGRRAVIGLGGTLLIGACVFAPVNHDTLPLAASLFLLG
                     LGWNCCFVAGS"
BASE COUNT          483 a          662 c          741 g          562 t
ORIGIN      
        1 tccacctacg atcgattatt cccgctattc tttggatcga atcagccgcc gatgctggac
       61 ggcacgggtg gcatgtcgag ccgcgatcaa gaaatgttgg ctgacgcgct gcggcagatg
      121 ttgaaggaac tgcgcgccga attgttgcgc cgtctggcgg aaggccggtc gcttactccc
      181 gaagaattgg aacaactggg acaaaacgct ggattgcaaa acgccaatca gcgttatcag
      241 cagcaatgga ttacgcgccg gatgctgcaa caacttgggc tgaatgattt ggctgaagcg
      301 atcgatgaat tgatggaact gcttgcggcg ttgggcatgg acgcgcaatc tcgccaggaa
      361 ttgcgcaatt cgatggatgt caatggcgaa gcgatcgagc agcaggtgaa agactacgtc
      421 gggcagcaga tcgccgagaa catggccaaa gagtatgaga aacgtgagcc gctcgacgat
      481 ctgatgcacc ggccgtttgc ctcgttgacc gaagaggaag ccgacgaact gcgtactcaa
      541 gttcaacgct tagccgcgca gttgcgcagc cgcgcggcct tgcggcagaa gcgcggcaaa
      601 gacggcacgc ttgatccaaa ggcgacagtg cgtgcgaatt tgaaatatgg cagcgtgccg
      661 atcgaaatga agttcaggca caagcaattg aaaccgaaac tcgtgttgat tgcggacgtg
      721 tccacttcga tgcggcccgt ggccgagttc atggtgcgct tgatgtacga actgagtgat
      781 cagatcgccc gggcccgatc gtttgcgttt atcaatcaca ttcacgaagt cagccaggat
      841 ttcgatcatg gccgcgcaag tgaagtggtg caggacgtgt tggaaaaaat tccgccgggt
      901 tattacaaca ccgatctcgg ttcggcgctg gccgatttca ctcacgacca ctttgacgcg
      961 gtggatcatc gcacaacagt aatctttctc ggggatggcc gcaacaactt caacaatccg
     1021 cgcctcgatc tgatcgatca gatcaaacgc cgcgcgaagc gcgtgctgtg gttcaacccc
     1081 gaacaccctc tgcagtggcg atctggcgat agcgatatgt tacaatacgc gccgctgtgt
     1141 gattcggtgc atcaagtcag caacttggct gagttggcgg ccgcagtcga tcggttgttt
     1201 gactgagttc gcacgtcatt gcgagccacc ggaggtggcg cggcaaccct tgcacctcaa
     1261 tgtcctgcga tggtgcatcc gcaggatgta ccacggagca gtgcaggtgt cttccgatcg
     1321 attgatcaat cccaaggaga ttgcttcatc gcatcgcttc tcgcaatgac gaagacgaaa
     1381 ttcctgatcc ccaatcctta ttccctgatc gcatgaccat ccattacaac tcaaatctcg
     1441 aatcgaccac gcgccgattg acgaccgtgc tgtttatcgg ccaaggcttg gcgctgctgg
     1501 cgatcttcac tacatcggcg atctccagca tcacgggcac aatgttgggc ggcacagcgc
     1561 gtgtagccgg ctggccatcg gcggctactt tgatcggcgg gtcgctcgcc gcgtatccga
     1621 tcggtcgttt gatgggccac gttggtcgac gcatcggttt gtcgatcggt tacgtgatcg
     1681 gcatattggg cggcattgtt gccggtctag gcgtaatcgc ccaatcgctg cccgttttct
     1741 tgatcggttc gatgctgcta ggcgcagcgc gcgtctcatc cgatcaagcg cgttatgcgg
     1801 cggccgatgt gtccacgttg cgcagccgtg cgcgcgcggt aagtctgatc gtttttgccg
     1861 gaacgattgg tgcggtgttc ggcccggcgc tggtcccgat cgccgatcgg gcagctcaag
     1921 cgatcggatt aagccagctc gtcgggccgt ggttgatcag cggcgtgctg ttcgtcatcc
     1981 cattgatcgt catcaatatt tttctgcggc ctgatccgcg cgacatcgcg cagcggttat
     2041 cgactgctaa cgatggaggc caagagactt taattctcgc gcccgcgcgc tcgctcttac
     2101 aggttttagc gcacccggcg gcgcgcgtcg ctttgatcgc tttagcattg gcgcaaattg
     2161 tgatggtcat ggtgatgacg atcacgccgg tgcatatgct cgattacgcg catccgctgg
     2221 aagacattag tctggtcatc tcggcgcaca ttgtggggat gtacggttta tcgatggtca
     2281 ccggctggct gagcgatcgg gtggggcgcc gcgcggtgat tggtttgggc ggcacgctgt
     2341 tgatcggcgc gtgcgtcttt gcgcccgtga atcatgacac gctgccattg gcggcttcgt
     2401 tgtttttgtt gggcttgggt tggaactgct gcttcgtcgc cggctcgg
//
LOCUS       DASRTJ010000424         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_329329,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000424 DASRTJ010000000
VERSION     DASRTJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329329"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1022
                     /locus_tag="VFF70_08660"
     CDS             237..1022
                     /locus_tag="VFF70_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY44805.1"
                     /translation="MINFHEVTYAYPNGRPVFDRFSGSIERGAAWAVVGASGCGKTTL
                     LMMLAGLRRPTHGSITINAEVIDRPRPQTGLVLQDYGLLPWATIAQNVALGLRLRNYY
                     GPDGRHAPIEHAVGPIDLRVNQWLKRLGIDTVRNHFPAQVSGGQRQRTAIARTLCLDP
                     DLLLMDEPFSALDAPTREDLQNLTIELQREQNLTLLVVTHNIEDAVFIGQKILVLGQP
                     PHIKALIVENPQAGDRSYRSSPKFFEMCTRVRELLTQGITAFH"
     gene            1044..1781
                     /locus_tag="VFF70_08665"
     CDS             1044..1781
                     /locus_tag="VFF70_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY44806.1"
                     /translation="MRRRNVILTSLIALIVWQIIAMLIQRDILPTPIQVAQSMVVNFG
                     ELSQHFVASALRVIISIIIAIALAVPAGLAMGQMPKVNRIFSPLVYIVYPIPKIVFLP
                     IILLFFGATDLSKVLTIVLILFFQILVPVRDDAASLRPELLNSVKSLGAGRRALFRFV
                     YLPASLPAVLTSLRLSVGTAVAVLYITETTATLLGLGYYIYLNYSTLFDYAATYAGAV
                     AMGMLGLVLYFLIDWLERKLCPWKFAN"
     gene            1850..>2024
                     /locus_tag="VFF70_08670"
     CDS             1850..>2024
                     /locus_tag="VFF70_08670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44807.1"
                     /translation="MTHLQGEARAKYVRDMFARIARRYDLMNRLMSVGRDGAWRREVI
                     KQAALNSNARILDV"
BASE COUNT          435 a          530 c          534 g          525 t
ORIGIN      
        1 gttcaacgat gtcgaagcgt ggatgaaggg caaaaatttg ctgacagccg atgtggcata
       61 tgccaagttg gtcgatgcga gttatttgcc gaagtagcct cactccctcc tatcctgacg
      121 atgaaatgaa agtcgattaa gcattgatcc aacgtatcgt caggataggg gacaaagggg
      181 tgaggaagag gatgggagat caagtataat gcctctcgtc ctcttttcga tctgacatga
      241 tcaactttca cgaagtcacc tatgcctatc ccaacggccg ccccgtattc gatcggttta
      301 gcggctcgat cgagcggggc gcagcgtggg cggtggtcgg cgcatcgggc tgcggcaaga
      361 ccacattatt gatgatgctc gccggattgc gccgtcctac acatggatcg atcaccatca
      421 atgctgaagt gatcgatcgg cctcgtccgc agaccggttt agtcctgcaa gattatgggc
      481 ttttgccctg ggcgacgatt gcacagaacg tagcgttggg cttgcgcctg cgaaactatt
      541 atggccccga cgggcgtcac gctccgatcg agcacgccgt tggcccgatc gatctgcgcg
      601 ttaatcagtg gttgaaacga ttaggcatcg atactgtgcg caatcatttc cctgctcaag
      661 tctccggcgg acaacgtcag cgcacggcga ttgcgcgcac gttgtgtctc gatcccgatc
      721 tgctcttgat ggatgaaccg ttctctgcac tcgatgctcc gacgcgcgaa gatttgcaaa
      781 atctcaccat cgaattgcag cgcgagcaaa acctcacgct actcgtcgtc acccacaaca
      841 tcgaagatgc ggtgtttata ggacaaaaga ttttggtgct tggccagccg ccgcatatca
      901 aagcattgat tgttgaaaac ccacaagccg gagatcgatc gtatcgatca agtccgaagt
      961 tttttgaaat gtgtactcgt gtgcgcgaat tgcttacaca aggcataact gcttttcact
     1021 aattactcag cactaatcac cttatgcgcc gtcgcaatgt tattctcaca tccctcattg
     1081 ccttgattgt gtggcagatt atcgccatgc ttattcaacg cgatattctc ccgacgccga
     1141 ttcaagttgc tcaatcgatg gtggtgaact tcggcgaatt gagtcagcac tttgtcgcga
     1201 gcgcactgcg tgtgatcatc agcatcatca tcgcgatcgc attggcagtg cctgccggtc
     1261 tggcgatggg gcagatgccg aaagtcaatc gcatcttttc gccgttggtc tatatcgttt
     1321 atcccatccc caagatcgtc ttcctgccga tcattctgtt attcttcggc gcgaccgatc
     1381 tcagcaaggt gctgaccatc gtcttgattc tattctttca aattttggtg ccggtccgcg
     1441 atgatgcggc cagcctgcgg cctgaattgc tgaactcggt caagtcgctc ggagcagggc
     1501 ggcgcgcttt atttcgattt gtatatttgc ccgcgtcgtt gcccgccgtg ttgacttcat
     1561 tacgcttgag tgtcggcaca gccgtcgctg ttttgtatat caccgaaacg acggcgacac
     1621 tgctcggcct aggctactat atctacctca attacagcac cttgtttgat tatgcagcca
     1681 cttacgccgg tgcagtcgcg atggggatgt tggggctggt gttgtatttc ttgatcgatt
     1741 ggctggaacg caaattgtgt ccgtggaagt ttgcaaattg atgagaggca aaatattagg
     1801 ggcgtaggtc agattgacct aatccctgat ccctaacccc tggttttcta tgacccattt
     1861 acaaggtgaa gcccgcgcga aatacgtgcg cgatatgttc gcccgcatcg ctcgacgcta
     1921 cgatcttatg aatcgcctga tgagcgtcgg gcgcgatggc gcttggcggc gtgaagtgat
     1981 caagcaagcg gcgctgaatt ccaacgcgcg cattctcgat gtag
//
LOCUS       DASRTJ010000425         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_162792,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000425 DASRTJ010000000
VERSION     DASRTJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162792"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFF70_08675"
     CDS             <1..224
                     /locus_tag="VFF70_08675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY44808.1"
                     /translation="PDVIVHGHNGWLIPPDDPTALHAALLTLLADADLRDRLGRAARE
                     RVVADNSLMGVTTKTRAVYDQVWLRDVHE"
     gene            297..683
                     /locus_tag="VFF70_08680"
     CDS             297..683
                     /locus_tag="VFF70_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44809.1"
                     /translation="MIPTLSVAQKLFIKEGHTVLSIHAPKNYPALLGELPQDSKLMTK
                     ASARVDAIHFFAHSRKYLEAHAPKLKGQLKPNGLIWIMYHKGTSKVKTDVNRDTLHAY
                     AKTIGLDGVSLISIDQDRSAMRFKVM"
     gene            complement(896..>1676)
                     /locus_tag="VFF70_08685"
     CDS             complement(896..>1676)
                     /locus_tag="VFF70_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44810.1"
                     /translation="QYRSPDAQITGVITLTSNNSTHGFAHLYAWSGDDGYSTSTAWIS
                     GTGTITAVYHLAALSGMDWQVVAVYETHNHYWITRTTVAVANPGPTFHDLMLSGPQLK
                     PAPVTVLLDPTQDRSIELSDGTRIFIPAGAISATGNVILHITPLANGSHEAHADPIDL
                     SYAFEAYTSDGQLITSNFNQDVVITFKVDPADLAARHLDLRHVRPAYYSTTTNSWTVP
                     DSFVIDESNHEITMQIDHFTRFGKMGMEGGGSDIFLPLVLR"
BASE COUNT          395 a          462 c          453 g          366 t
ORIGIN      
        1 cgccggatgt aatcgtccac ggccacaacg ggtggttgat cccgcccgat gatcccacag
       61 cgctgcatgc ggcactgcta acattgttgg ccgatgctga tctgcgcgat cgattagggc
      121 gcgcggcgcg tgagcgggtc gtcgcagata attcgttaat gggtgtcacg actaaaacgc
      181 gcgcggtgta cgatcaagta tggctgcgcg acgtgcatga atgaaaaagc aaaaatgtgg
      241 cgcgatcgct tgagctatcc gcgttcaggc ttatagtcta ttttatttgt gacattatga
      301 tacccactct atccgtcgcg caaaaactat tcatcaaaga aggccacacc gttctctcga
      361 tccacgcgcc gaagaactac ccggcgttgc tcggcgaact gccgcaggat tcgaagttga
      421 tgacgaaggc cagcgcgcgc gtcgatgcga tccacttctt tgcccactcg cgcaaatatt
      481 tggaggcgca tgcgccgaaa cttaaaggcc agctgaaacc caacggccta atctggatca
      541 tgtatcacaa aggcacctcg aaggtgaaga cggacgtcaa ccgtgacact cttcacgcgt
      601 atgcaaagac cattggatta gatggtgtgt cgctgatctc gatcgatcaa gataggtcgg
      661 cgatgaggtt taaggtgatg tgaaaaccga ttgtcatttc gagccgcaca cctacaacgc
      721 agtgctcccg tccgctcgcg gcgaaaaatc tcagattgca tcagcgtcaa gtgtgagtca
      781 gggcaaagat tactcactgc gttcgaaatg acaggctaaa tggtaagagc cgctcctttt
      841 agagcggctc tttttattgc atgtaggggc gagatcacct cacccacact gaaacttatc
      901 tcaacaccaa cggcaggaaa atatccgagc cgccaccttc catgcccatc ttgccgaagc
      961 gtgtgaagtg atcgatctgc atggtgattt catgattcga ttcatcaatc acaaaactat
     1021 ccggtaccgt ccagctgttg gtcgtcgtcg agtagtacgc cggtcgaacg tggcgcagat
     1081 cgagatgcct cgcagccaga tcagccggat cgaccttgaa ggtgatgacc acatcttgat
     1141 tgaagttcga agtgatcaac tgcccgtccg aagtgtaagc ctcgaaggca tagcttaaat
     1201 cgatcggatc ggcgtgcgct tcgtgcgaac cattcgccag cggtgtgatg tgcaaaatca
     1261 cattgcccgt cgccgagatc gcgccggccg gaatgaagat gcgtgtcccg tccgaaagtt
     1321 cgatcgatcg atcttgagtg ggatcaagca gcaccgtcac cggcgcaggc ttgagctgcg
     1381 gcccggacag catcaagtcg tgaaacgtcg gccccggatt cgcaacggcc accgtcgttc
     1441 gagttatcca gtaatgattg tgcgtttcat acaccgcaac cacctgccag tccatccctg
     1501 acaacgcggc gagatgatac accgccgtga tcgtacccgt gccgctgatc cacgccgtcg
     1561 aggtcgagta accatcatcg ccgctccatg cgtacagatg tgcgaagcca tgcgttgagt
     1621 tgttgctggt caacgtgatc acgccggtga tctgcgcgtc gggcgatcga tattgc
//
LOCUS       DASRTJ010000426         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_101761,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000426 DASRTJ010000000
VERSION     DASRTJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101761"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..596)
                     /locus_tag="VFF70_08690"
     CDS             complement(153..596)
                     /locus_tag="VFF70_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44811.1"
                     /translation="MNQSATSRRVLDIIVLAILLAGAGFVMYNLLPQVTKPNEMVVPP
                     LPASAFDPVQAFLTVFITMTAVGAPVTGAIVLALILKGVSKSIPLNAATPDRPIEKKK
                     VDPAGVKELSKGEATFWKIAATLLILAAIGGLTAVMWPTFVQWFS"
     gene            complement(647..1132)
                     /locus_tag="VFF70_08695"
     CDS             complement(647..1132)
                     /locus_tag="VFF70_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44812.1"
                     /translation="MPKQIMNWIRHVRSAGWLAGVTLFARAAQDGQPPADQTQNTVAI
                     IIVIAAIALPLLMGGGFALMTWLISLGTPTPLVAVDQKRIIKREILPEGAHMPPPSSR
                     PIMLALGMTLICIGILLRGVAIALSPDFNVPIILVIGALITFLALLGWIRDDLRAAKG
                     H"
     gene            complement(1448..>2992)
                     /locus_tag="VFF70_08700"
     CDS             complement(1448..>2992)
                     /locus_tag="VFF70_08700"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZY44813.1"
                     /translation="VHIVLGAVTVLLQNAPFTVALHLGTALAMFAFATIVAAVARQSG
                     ESNPLIFNLRHNRLLSQSIASALLVYVLILIGALVTATGSALACLDWPLCQGQLLPAS
                     SDSSVYIQWIHRFVALIAGIVLLRLTIVTWRKRLQQPQIWIVAAAGLSLYIVQVTIGA
                     GNVLLRVPLVLRGLHLAVAAAVWACVVVLSVLAARKSNLASASDPMQVNEGMPNRLSA
                     EPIVDLNRPPFRSVAGAYFKLTKPWILILLLVTTFAAMLIAQRGLPPLPLVFFTLLGG
                     ALSAAGASSINSYIDRDIDGLMSRTKNRPVPAHRIEADQALAFGLILSVTAFVVLTLF
                     VNLLSAILATIGLLFYVFVYTGWLKRSTPHNIVIGGIAGAIPPLVGWAAVTGRVEVTA
                     LYLFLIIFYWTPPHTWALMLLVSGDYAKVGVPMLPVVRGDAVTRQQIVLYSVQLVAIT
                     LLLFAFQLMGWVYFISALLLNGLFLWLVLVLARDKGKAAAKRLYKFSQLYLALLFLAM
                     VIDKLV"
BASE COUNT          775 a          864 c          763 g          590 t
ORIGIN      
        1 gccgatcccc ggcatcgatc cgggttgatc catggcgtgt tcgtgggttg atgctgcatc
       61 actcatgtgg aatgtctcct ccccagaaag ccgtgcgagg gttgaatcga tcgatcgtca
      121 gtcgaccctc gaaccctcgc caggttgtta atttagctaa accactgtac aaaagtgggc
      181 cacatcaccg cggtcaatcc accgatggcg gccagaatca acagcgttgc ggcgatcttc
      241 cagaacgttg cttcaccttt cgacagttct ttcacgcccg ccggatcgac tttctttttc
      301 tcgatcggcc gatcgggcgt agcggcgttt aacggtatgc tcttgctgac gcctttgaga
      361 atcaaggcca gcacaattgc acctgtcacg ggtgcgccaa ctgcagtcat cgtgatgaaa
      421 acagtcagaa acgcttgtac gggatcaaaa gcgctggcgg gcagtggcgg cacgaccatt
      481 tcattcggct tggtgacttg cggcagcaag ttgtacatga cgaagcctgc accggcgagt
      541 agaatcgcca gcacaataat atccaacacc cttcgacttg tagctgattg attcataacg
      601 atctccttcc agtcgatcga agtcaagccc gaccgggctg agctgcttaa tgtcctttgg
      661 ccgcgcgcaa gtcatcgcga atccaaccga gcaaggccag gaaagtgatc agcgcaccaa
      721 tcaccaagat gatcgggaca ttaaagtccg ggctaagcgc gatcgccaca cctcgcaata
      781 aaatgccgat acagattaag gtcatcccta acgctaacat gatcgggcgg ctgctcggcg
      841 gcggcatgtg cgcgccttcc ggcaggatct cgcgcttgat gattctcttc tgatcgaccg
      901 ccaccagcgg cgtcggcgtg ccgaggctga tcagccacgt catcagcgcg aagccaccgc
      961 ccatgagcaa cggcagggcg atcgcggcga tgacaataat gatcgcgacg gtgttctggg
     1021 tttgatcggc cggcggttgt ccatcttgag cggcgcgcgc gaacaaagtc acgcccgcca
     1081 accaacccgc tgatcgaacg tggcgaatcc aattcattat ttgttttggc atgtaaacct
     1141 cagtcgatct atgattgctg atttttgatc tcagtacttg cgcttagctg tcatttcgag
     1201 catttgcttc ggcaggctca ggacaagtat tgcgagaaat ctttacgatc atcaacatgt
     1261 cttgtctatc aggacggaga tttctcactt cgttcgatat gacaaacgac aatcctgcct
     1321 ctgcttcaac gaccgagcac aacagcccgg gttaaaatca tcgacgcgat gagcatcacg
     1381 gcaaagatca ctaataaaat tgcggttagt ttggcgttgc ggcgtgcagt tgatttatcc
     1441 atggtcctca tactagctta tcgatcacca tcgctaagaa caacaacgcc agatacaatt
     1501 gcgagaactt atataaacgc ttggccgcgg ctttgccttt atcacgcgcc agcaccagta
     1561 ccagccacaa aaataaaccg tttagaagca aggcgctgat aaagtatacc caacccatca
     1621 gctgaaacgc gaacaacagc aacgtgatcg cgaccaactg caccgagtac aacacaatct
     1681 gctggcgcgt cacggcatcg ccgcggacaa ccggcagcat cggcacgccg actttcgcgt
     1741 aatcaccgct caccaataac atcaacgccc aggtgtgcgg cggagtccaa tagaaaatga
     1801 tcaagaataa atacagcgca gtgacttcca ctcgaccggt cacggcggcc cagccgacca
     1861 gcggcgggat cgctccggcg atgccgccga tcacaatgtt gtgcggcgtc gatcgtttga
     1921 gccagcctgt ataaacgaac acatagaaca gtaaaccgat cgtcgccaag atcgcgctga
     1981 gcaagttaac aaagagagtc agcaccacaa atgctgtgac gcttaagatc aaaccaaacg
     2041 ctaaagcttg atcagcttcg atgcgatgag cgggcaccgg tcgattcttc gtgcggctca
     2101 tcaagccatc aatatcccga tcgatatagc tattgatcga gctggccccc gcggccgata
     2161 gtgcgccgcc taataacgtg aagaacacca acgggagagg aggcaagcct cgttgggcga
     2221 tcaacatcgc cgcgaaggta gtgacgagca gcaagatcag aatccacggc ttggtcaact
     2281 tgaaatacgc gcccgccact gatcgaaagg gcggtcgatt cagatcgact atcggctcgg
     2341 cggacaatcg attcggcatg ccttcattga cctgcatcgg atcagaggct gatgccaaat
     2401 tactcttgcg cgcagccaac acactcaaca ccacgacaca cgcccagact gccgcagcta
     2461 cggcgagatg caagccgcgt aaaaccaacg ggactcgcag caaaacattg cccgcgccga
     2521 tcgtgacctg aacgatatac aagctcagac ctgctgccgc gacgatccaa atttggggtt
     2581 gctgcaggcg tttgcgccaa gtgacgatcg tcaaccgcaa caaaacaatg cccgcgatca
     2641 atgccacgaa gcggtgaatc cactgaatgt aaacgctgga atcgctgctg gctggcaaca
     2701 actgaccttg acacaacggc cagtccaaac aagccaaggc cgatccagtg gctgtcacca
     2761 gcgcgccgat caagatcaac acgtatacga gcagcgcact cgctatagac tgcgacaaca
     2821 atcggttatg ccgcagatta aaaatgagcg gattggattc accagattgt cgggcgacgg
     2881 cagcgacgat cgtcgcaaaa gcaaacatcg ccagcgcggt gcccaagtgc agcgcgacgg
     2941 tgaaaggcgc attctgcagc aaaactgtca ccgcgcccaa aacgatatgg ac
//
LOCUS       DASRTJ010000427         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_352018,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000427 DASRTJ010000000
VERSION     DASRTJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352018"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFF70_08705"
     CDS             <1..484
                     /locus_tag="VFF70_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY44814.1"
                     /translation="VTVSTLHAGTAFNITADTAEMLGTIRTFDPVVRELIVQRVTEIV
                     EHTARAMGGSASIEIKAISPTTVNDERVASLVRDTAIDVLGQENVSADQFTMGAEDMS
                     EFLNRVPGCFFFIGSANEQKGFTAPHHNPHFDIDEDVLPMGAAVLATAAVKYLNGQLG
                     "
     gene            525..2036
                     /locus_tag="VFF70_08710"
     CDS             525..2036
                     /locus_tag="VFF70_08710"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZY44815.1"
                     /translation="MNPKFDELKNRLMEVNDINSAASVLNWDQLTYMPPGGAPARGRQ
                     LATLSRIAHEKFIDPAIGRLLDDLRSDEESLPYDSDEASLIRVARREYEKSIKVPSEF
                     IAELNQHSTAAYQAWAAARPANDFAKVLPYLEKTLDYSRRLANYFPGYAHIADPLIDF
                     SDHGMTVAIIRPLFTRLLEQLVPLVQAITDQAPADDACLHQAFHEDQQLAFGTELIAA
                     IGYDFNRGHVDRTLHPFMTKFSLGDVRITTRFDKNFFGDTPFSMLHEAGHALYEQGIR
                     MDFEGTPLAGGTSSGVHESQSRLWENLVGRSRSFWEFFYPKAQAAFPAQFKNISLDHF
                     YRAINSVRRSLIRTESDEVTYNLHVMIRFDLEVEMLEGGLAVRDLAEAWRERYRTDMG
                     LAPKDDKDGVLQDVHWYGGTIGGSFQGYTLGNIMGAQFFAAANQAQPALQKDIAHGQF
                     ADLLTWLRENIYQFGRKFTATEIVQRVSGGPLSIDPYIQYLRTKYGELYKLRS"
     gene            2033..>2302
                     /locus_tag="VFF70_08715"
     CDS             2033..>2302
                     /locus_tag="VFF70_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44816.1"
                     /translation="MTQAGPLQWRSGSGWLVLIGGGKWAENETIHSAAISALVDESPI
                     AFVPAANPDPLYGAAFLSYYAELGAPTGYVVPIHDQASAHDPANYR"
BASE COUNT          502 a          617 c          672 g          511 t
ORIGIN      
        1 ggttaccgtc agcacgctgc atgcgggcac cgctttcaac attacggctg acacggccga
       61 aatgttaggg accattcgca cattcgatcc ggtagtgcgt gagctgatcg ttcaacgtgt
      121 gacggaaatt gttgagcaca ctgcgcgcgc catgggcggt tcagcctcga tcgagatcaa
      181 agcgatctcg cccacgaccg tgaacgatga gcgggtggcg agtttagtgc gcgacaccgc
      241 gatcgatgta ttgggtcaag agaatgtatc cgccgatcaa ttcacgatgg gcgcagaaga
      301 catgagcgag tttctcaatc gtgtaccggg ctgcttcttc ttcatcggct cggccaatga
      361 gcagaaaggc tttactgcgc cgcatcacaa tccgcatttt gatatcgacg aagatgtctt
      421 gccgatgggc gcggcggtgt tagccacggc ggccgtgaaa tatttgaatg gccagttggg
      481 ctgagttgac ctcgtccgaa taattgttga atggagaaaa ggcattgaac ccaaaatttg
      541 acgaacttaa gaaccgtttg atggaagtca acgatatcaa ttccgccgcg agcgtgctga
      601 attgggatca gttgacttac atgccgcccg gcggcgcgcc cgcgcgcgga cggcagttgg
      661 ccacgttgtc acgcattgcc cacgaaaaat ttatcgatcc ggcgatcggc aggctgctgg
      721 acgatctgcg atcagatgaa gagagcctgc cttacgattc ggacgaggcg agtttgatcc
      781 gtgtggcgcg ccgcgagtac gagaagtcga tcaaagtccc gtcggagttt attgcggaac
      841 tgaatcagca cagcacggct gcttatcaag cctgggcggc cgctcgacct gcaaatgatt
      901 ttgcgaaagt gctgccgtat ctggaaaaga ctttggatta cagccgccgc ctggcgaatt
      961 attttccggg ctacgcgcac attgccgatc cgctgatcga tttttccgat cacggtatga
     1021 cggtggcgat tatccgcccg ctgttcacgc gtctgctgga gcagctggtc ccgctcgtgc
     1081 aggcgatcac cgatcaagcc ccggccgatg atgcgtgctt gcatcaagcg ttccacgaag
     1141 atcaacagct cgccttcggc acggagttga tcgcagcgat cggttacgat ttcaatcgcg
     1201 gccatgtcga tcgaacgctg catcccttca tgaccaagtt ctcgctgggc gatgtccgca
     1261 tcacgacgcg tttcgataaa aatttctttg gcgatacgcc ttttagcatg ctgcacgaag
     1321 ccggccacgc tttatacgag cagggcattc gcatggactt tgaaggcacg ccgttggctg
     1381 gcggcacatc ctcgggtgtg cacgaaagcc aatcgaggtt atgggaaaat ctggttggac
     1441 gcagtcgcag cttctgggag ttcttctacc ccaaagcgca agcggccttc ccggctcaat
     1501 tcaaaaatat ttcgctcgat cacttctatc gggcgatcaa tagcgtgcgc cgatcgctca
     1561 tccgcaccga atcggacgag gtgacttaca acctgcatgt gatgatccgc ttcgatcttg
     1621 aagtcgaaat gttggaaggc ggcctggccg tgcgcgatct ggccgaagcc tggcgcgagc
     1681 gctatcgaac cgatatgggt ctggcgccaa aggacgataa ggacggcgtg ctgcaagatg
     1741 tgcattggta tggcggcacg attggcggat cgtttcaggg ttacacgctg ggcaacatca
     1801 tgggcgcgca gttctttgct gccgccaatc aggcgcagcc cgctctgcaa aaagacattg
     1861 cccatggaca atttgccgat ctgttgactt ggctgcgaga aaacatctac cagtttggcc
     1921 gcaagtttac ggcgacagaa attgtgcagc gggtgtcggg tgggccgttg agcatcgatc
     1981 cgtacatcca atacttgcga acgaaatacg gcgagttgta taaactcaga tcatgacgca
     2041 agcagggcca cttcaatggc gatcaggctc cggttggctg gtcttgatcg gcggcgggaa
     2101 atgggcagaa aatgaaacga ttcactccgc ggcgatctcg gccttagtcg atgaatcgcc
     2161 gatcgcattt gtgccggccg ctaaccccga tccgctttat ggcgcagcgt tcctgtcgta
     2221 ttacgcggag ttaggcgcgc cgaccggcta tgtggtgccg attcacgatc aagcctcggc
     2281 acacgatccg gccaactatc gg
//
LOCUS       DASRTJ010000428         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_162847,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000428 DASRTJ010000000
VERSION     DASRTJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162847"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFF70_08720"
     CDS             <1..219
                     /locus_tag="VFF70_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44817.1"
                     /translation="GVLLVLAALLLQQAVPWLHVIVMLPALVVGHLNRRVRDRPDRVR
                     HQVQLGVYIGTLALSVIGLAIINQLALP"
     gene            281..934
                     /locus_tag="VFF70_08725"
     CDS             281..934
                     /locus_tag="VFF70_08725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZY44818.1"
                     /translation="MSPAIAFFDMDYTILDASSSLLYVKYLRRRGHLKRRQLLRVGWW
                     AILYKFSAMDLVAIMPKILSFANTLNARETFEQAQTWFKDMVAVHIAPKAVQQIREHQ
                     QHGQRVAIISASSQFAVQPVAHALGLDFLCTQLEIKDDQLTGRVFEPVCFGVGKIYWA
                     KQYAEQHAAQLSDCYLYTDSYTDRPLLEIIGHPVAVNPDPRLKRLARRRRWPIVNFY"
     gene            1080..1991
                     /locus_tag="VFF70_08730"
     CDS             1080..1991
                     /locus_tag="VFF70_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZY44819.1"
                     /translation="MILITGATGFIGQHLIAWLAGHQYPICALIHPSQRERRFAPGIS
                     LQLVSGDVTNPVVLRMAMHHVDTVFHLAGIDAESAAGAFDAINVEGTRNVIDAMREAG
                     VTRLITLSPIGADPHSAYPYLRSKGLAAELITHSGLDFTLIRSSAVYGSGDDWTETIG
                     LALRRLPFIFPIAGDGRSRLQPISMTDLITCLDTCLTDPQTIKQTYTIGGPQHMTFEE
                     IVNTIGEATHHRRRPRYLRSPTALRFAKFIRSLLNGRLLYTTTDLDLLAIDRTTTLDS
                     VAYQFDFTPARMSASLDYLLARTRRVR"
     gene            2007..2900
                     /locus_tag="VFF70_08735"
     CDS             2007..2900
                     /locus_tag="VFF70_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44820.1"
                     /translation="MADFEIRALHSIVEFHAVEDLQRAVWNSDDLDVTPTHVLLTAAK
                     NGGIVLGAFSGSQLIGFVFGFLGAGEHRFGSEAPIAVRLKHCSHQMGVLPEWQGRGVG
                     YALKLAQRDAVHDQSLRLITWTYDPLESKNARLNILKLGAVSNTYLRNVYGDLRDGLN
                     HGLATDRFQVDWWIASRRVETRLKQQRPPLQLHHYTQAGTTILNPAQFDENGLLVCAE
                     NVKPLPADRVLLEFPSDFQAIKKTKNDLALAWREQIRSICEHAFKRGFSVIDFVYEPG
                     PPARSFYLLQMIESSESTLSS"
     gene            3006..4112
                     /gene="menC"
                     /locus_tag="VFF70_08740"
     CDS             3006..4112
                     /gene="menC"
                     /locus_tag="VFF70_08740"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZY44821.1"
                     /translation="MIINRIELRIVSMQLTAPFETSFGVEHDRDCVIVRVEADGLTGW
                     GECPVSRGFGYSYETTTTAWHALKDFLVPQLLGKPVDVRQAVEIGELLRGHNMARHGL
                     EAALWDIVAQSNNISLSRALGGTRDRVAVGVSIGIQESADVLIKKIEDYLAQGYGRIK
                     IKIKPGYDLEIVKRVRAKFPKILLQVDANSAYRMPDVPLFEAMDDYKLLLIEQPFGWD
                     DIYEHSKLQPHLHTPICLDESIHSLGHAVMAIELGACKIINIKPARVGGFTESRRIHD
                     LCQSRKMPVWCGGMLETGIGRAGNIALASLANFTLPGDISASNRYYVEDIAEPLFTLN
                     PDSTLTVPTAPGLGVTVNMKNLEKYTIRKEMFTL"
     gene            4187..>4687
                     /locus_tag="VFF70_08745"
     CDS             4187..>4687
                     /locus_tag="VFF70_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZY44822.1"
                     /translation="MHLEVAIAKIRKYATPESGDTIEMIERPGGGLSFVVIDGQRSGK
                     SAKAISNVVARKAVSLLAEGVRDGAVARAASDYLFTQRSGKVSATLNIVSVDLESRSL
                     VLSRNSHCPIVVIKNNRASLLNELSHPVGVQRGTKPIIAEIPLAADTTVVIYTDGLLS
                     AGERSGQ"
BASE COUNT         1076 a         1254 c         1240 g         1117 t
ORIGIN      
        1 ggcgtgttgt tagtgcttgc tgcgttgctg ctgcaacaag ctgtgccgtg gctgcatgtg
       61 atcgtgatgc taccagccct cgtcgtcgga catctcaatc ggcgtgtccg cgatcgaccc
      121 gatcgagtcc ggcatcaagt gcagctaggt gtttacatcg gcacgttggc attgagcgtg
      181 atcgggttgg cgatcatcaa tcaattagcg ctgccataat ctcgacgact tacctgttac
      241 acttcacgca ttactcgtta tttatcaccg attacttatc ttgtcacccg ctatcgcttt
      301 cttcgacatg gactacacta ttctcgatgc cagctcgagt ttgctgtacg tcaaatatct
      361 acgtcggcgc ggacacctca aacgccggca attgttacgc gtcggctggt gggcgattct
      421 gtataagttt tcggcgatgg acctggttgc aatcatgcct aaaattctga gctttgccaa
      481 tacgcttaac gcccgtgaaa cgttcgagca agcgcagacg tggtttaagg atatggttgc
      541 agtccatatc gcgccaaaag ccgttcaaca aattcgagag catcaacaac acggacagcg
      601 cgtcgcgatc atttcggcct cctcgcaatt tgccgttcaa ccggtcgccc atgcgctcgg
      661 cctcgacttc ttatgcacgc agttggaaat caaagacgat caacttaccg gccgtgtctt
      721 cgaaccggtc tgctttggcg tgggcaaaat atattgggcg aagcagtatg cggaacaaca
      781 tgccgcgcag ttaagcgact gctatctcta tacggatagt tataccgatc ggccgctgct
      841 ggagatcatc ggccaccccg ttgccgtcaa tcctgatccg cgcttaaagc gattggcccg
      901 ccgccgccgt tggcccatcg tcaattttta ttaaaccttg acggtcattc tgagcgcagt
      961 gaagaatctc ggcttctgtt aaaaataatc cagcctgcag tagagattct gaatcgcgct
     1021 tcgcgcgatt cagaatgaca cggcacgcat catgatataa tcgccacttg gataacaaaa
     1081 tgatcctcat cacaggtgca acaggcttca tcggccaaca tctcattgca tggctagccg
     1141 gtcaccaata tccaatctgc gcgttgatac atccctcgca gcgtgagcgc cgttttgcgc
     1201 cgggtatatc gctgcagctg gtaagcggtg atgtcaccaa tccggttgtg ctgcgcatgg
     1261 cgatgcatca tgtcgatacc gtgtttcatc tggccgggat tgatgccgag agcgccgcag
     1321 gagcatttga cgcaataaat gtcgaaggca cccgcaatgt catcgacgcg atgcgcgaag
     1381 ccggggtcac tcgattgatc accctcagcc cgattggtgc tgatccgcat tctgcatatc
     1441 cctacttgcg cagcaaaggt ctcgccgctg aattaatcac acatagcgga ctcgatttca
     1501 cgttgattcg atcgtccgct gtctacggct ccggcgatga ttggactgaa accattggat
     1561 tggcgttgcg ccgcctacct ttcatcttcc caattgccgg cgatggccgc agccgcttgc
     1621 aacctatttc aatgaccgat ctgattacgt gcttggacac ttgtctcacc gacccccaga
     1681 cgatcaaaca aacgtacaca attggcgggc cacagcacat gacgtttgaa gaaatcgtca
     1741 acaccatcgg tgaggccacg caccatcgac gccgaccgcg ctacctgcgc tcacccacgg
     1801 cgctacgttt cgcaaaattt attcgcagct tgttgaatgg ccgcttattg tataccacca
     1861 ccgatctcga tctgctggcg atcgatcgca cgacgacgct ggattccgtc gcatatcaat
     1921 ttgattttac accggcacgc atgtccgcct cgctcgatta tctactggca cgcactcgcc
     1981 gcgttcgcta gatttatcct tcaatcatgg ctgattttga aattcgcgcg ctgcattcca
     2041 ttgttgaatt ccacgctgta gaagatttgc aacgcgccgt ttggaacagc gatgatctgg
     2101 atgtcacgcc tacccacgtc ctgctgactg cggccaagaa tggcggcatc gttttaggcg
     2161 cgttcagcgg atcacaactg atcgggtttg tgtttggctt cttgggcgca ggtgaacatc
     2221 gcttcggatc ggaagcgccc atcgccgttc gattaaaaca ttgttcacat caaatgggcg
     2281 tgctgccgga atggcaaggc cggggcgtag gctacgcgtt gaagctggct cagcgagatg
     2341 cggtgcacga tcaaagcctg cgtttgatca cttggactta cgatccgctc gaaagcaaaa
     2401 atgcgcggct gaatatttta aaacttggcg cggtgagcaa cacctatctg cgcaatgtgt
     2461 atggcgatct gcgtgacggc ttgaatcacg gcctggcaac tgatcgattt caagtggatt
     2521 ggtggatcgc cagccggcgt gtggaaacgc gcttgaagca gcagcgtcca cctttgcaac
     2581 tgcatcatta cacacaagcc ggcacaacaa tcttaaatcc ggctcagttc gatgaaaacg
     2641 gcttgctggt ttgcgcggag aacgtaaagc cgctgccagc cgatcgggtg ttgttggaat
     2701 tcccgagcga tttccaggcg atcaagaaaa cgaaaaacga tctggccttg gcgtggcgtg
     2761 aacaaatccg atcgatctgc gagcatgcct tcaaacgcgg cttcagcgtg attgattttg
     2821 tgtatgaacc gggaccgcct gcgcgaagtt tttatctgct gcaaatgatc gagtcgagtg
     2881 aaagcacgct gtcctcatga cggggtgcgt tgtccgtttt ctacgcaata cgcaccacgc
     2941 aatacgtttt tacccatgac gttatcacat atctttcgcc cgcgagtcgc gggtttggag
     3001 caagcatgat catcaatcgc attgaactgc gcatcgtgag tatgcaactg accgcaccgt
     3061 tcgaaacttc gttcggcgtt gagcacgatc gcgattgcgt gatcgtccgc gtagaggcag
     3121 atggattgac aggctggggg gagtgcccgg tctcacgcgg cttcgggtat tcttacgaga
     3181 caacaacaac cgcatggcac gcgctaaaag attttctggt gccgcaatta ttggggaagc
     3241 cggttgatgt taggcaagcc gtcgagatcg gcgagttgct gcgcgggcat aacatggcgc
     3301 ggcacggact tgaagcggct ctatgggaca ttgtagctca gtcgaataac atttcgttat
     3361 caagggcgct cggcggcaca cgcgatcgag tagcggtcgg cgtgagcatc ggcattcaag
     3421 aatcggcgga cgtgttgatc aaaaagatcg aagattattt ggcgcagggt tatggccgca
     3481 tcaagatcaa gatcaaaccc ggttacgatt tggagattgt caagcgcgtg cgcgccaagt
     3541 tcccgaagat tctactgcaa gtcgatgcga actcagccta tcgaatgccg gatgtgccct
     3601 tgttcgaagc aatggacgat tacaagttac tgctgattga acagccattc ggttgggatg
     3661 acatttacga acattccaaa ttgcagccgc atcttcatac gccgatctgt ctcgacgaat
     3721 cgatccattc gctcggacat gcagtcatgg cgattgaact gggcgcatgc aagatcatca
     3781 atattaaacc ggcacgtgtc ggaggcttta ccgaatcgcg gcgtatccat gatctttgtc
     3841 agtcgcgcaa gatgcccgtc tggtgcggcg gtatgctcga aaccggcatc ggtcgagcgg
     3901 gtaatatcgc gcttgcttcg ctcgccaact tcacactgcc cggcgacatc agcgccagca
     3961 atcgatatta cgtcgaagat attgccgagc cgttgtttac cttgaatccc gattcgacat
     4021 tgacagttcc gactgcgccg ggtttgggcg tcacggtgaa tatgaagaac ttggagaagt
     4081 acacaattcg gaaggaaatg tttacactct gatatatgat aacgtgaggc gtgaaagaca
     4141 gttgagcaat cacgcatcac tcgtcactca tcactcatca ctcattatgc acctagaagt
     4201 cgcgattgcc aagataagaa aatatgccac gccggaaagc ggcgatacga tcgagatgat
     4261 cgaacggcct gggggcggat tgtcgttcgt cgtcatcgat ggacaacgat ccggcaagag
     4321 cgccaaggcg atcagcaacg tcgtcgcacg taaggcggtg tcgttactgg ccgaaggtgt
     4381 acgcgatggt gccgtcgccc gggctgcatc cgattattta tttacgcagc ggtctggcaa
     4441 ggtctccgcc acgctcaaca tcgtctcggt cgatctggaa tctcgatcgt tggtcctgtc
     4501 gcgcaacagc cattgcccaa ttgttgtcat aaaaaacaat agggcaagtt tgctgaatga
     4561 actatcgcat ccggtgggcg tccaacgcgg caccaagccg atcattgccg aaattccact
     4621 ggctgccgac acaactgtgg taatatacac ggacggacta ttaagcgcgg gagaacgatc
     4681 ggggcaa
//
LOCUS       DASRTJ010000429         8225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_101959,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000429 DASRTJ010000000
VERSION     DASRTJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101959"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="VFF70_08750"
     CDS             <1..1870
                     /locus_tag="VFF70_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44823.1"
                     /translation="FMIGIVQQIIEPIPVSASSSDEAIALENARLFAQTQQELSDRQQ
                     TEQAVQRRADQLAALNRIGRQLVQLAPASEILETIYQSIGQLIDNRNFYLALHDEGDP
                     EIHYPIYTFDNVRSEPFSRAYGQALTEHVLTTQQPILIPQDVNGYLAQIGVESVGRVA
                     QCWMGAPLLVGKQAIGVIALQDYERSNAYTQSDVELLTLVASQAAIALQNTRLFTQTQ
                     ARVDELAVLNQIGQAASGLLNVDELLELTYRQVSQVMRTDGFSVGLYDPNREMISYPL
                     CYADSEIFHEPDSVMVPDTHSYQVITTGQPLMIKYSEADRQTTLQQPEPLIGDKVGLP
                     ASVLFAPLKIGERVLGFMTVQSYEAGKHTTDNHNLLISIANQVAISLENARLFEQTRS
                     RADELSVLNELAQRLSARLSTDQVLDETYQGIKRLMDAENFYIGLYDQPKHQIYFMLN
                     VTQSEADREITRISADDGISGYIIRNRTSVLVGNGINQWAQKMGMRTVGQNALSWLGV
                     PMIVGNQVLGVMAVQNYEKAAAYSEHDRELLAVFANQAATAIQNALLFERTQRDGQRE
                     RTINRISAKIRNTQSVEQILRIATEELRLATQSARSIAQIAPAAATPSDSSSKSSN"
     gene            1901..2092
                     /locus_tag="VFF70_08755"
     CDS             1901..2092
                     /locus_tag="VFF70_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44824.1"
                     /translation="MKRNDSAPTATDRLRSSVALQTRNGLIAPIALYCEVVGARDVRI
                     RLGVDASPGDGNGRQNDHA"
     gene            2085..3965
                     /locus_tag="VFF70_08760"
     CDS             2085..3965
                     /locus_tag="VFF70_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44825.1"
                     /translation="MPNLLTRWLTTETRRYVALGAAFGLVFPLIATLVTLILRQLPIS
                     VSNMWAVQTTDPLLWIIDTAPFFLGAFSGLAGRRQDALQSTNQRLREATHALEKQEQR
                     QADELGRQAAQLKASADVSRAAASTLDREELLTKITELIVERFGFYYAAVFLLDASGE
                     NVVLHKATGEAGRILKERGHKLPVDNASMVGSAITTRKPRIALDVGKERVRFANPLLP
                     NTRSEIALPLVVGDHALGALDVQSIQEAAFDDANAAVLQSMADLIAVALNTATSFSQA
                     RTETQRAKQIATLLGSTLELTLHSDRQTVYDRIVRIGMDLVNADGAGLALPISDTELE
                     MKTSIEVGSDPQQMTGRRLKINESISGRVFATKQTLRIDNYPTWSGRSQAFTDAPSQA
                     ILGVPLVWQDRVLAVLTITHSQPNQPFTEDDEFSIKLLAAQGAATLANVDLRENQAQT
                     LNELNALNLRLTRETWTAFSKQLKREGVDWIGMGEPIGPDRQPEVSEALSSGRIATNS
                     ESDQNQTAVAIPIVLRGVPIGVMRLRVPSENWSSSTASTLTSIAGHIAQAIENARLLQ
                     VTEERFARERDLSESTDKIRRKTDVEQILQTAAEELARHLQATRVAVSIGPQPSAESD
                     RL"
     gene            3973..6666
                     /locus_tag="VFF70_08765"
     CDS             3973..6666
                     /locus_tag="VFF70_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44826.1"
                     /translation="MSQLPKLPQSNKPFTATGGIGRTLLLSFLILTLVPLTLVLVIGT
                     NISFQTAQADAFARLSGVSEIKVQAIEDWLGERQNNLLDILGIAGTPQLLQDILRNPN
                     NNTAAAALATNLSDEVREHQVFARFIVLDAAGHSLISTDPGLVGQSYSSETYFTRGLL
                     GPFITSAQNDMTGRYDTIVVAAPVVNAAKQTVGVLVAEANIDQLSRIVLTSLGTNRTD
                     DSYLVGPDRRFLTQSHYLHTENSGDIVETEAVIAALNDPAAIGQATYNNYINQPVLGV
                     YRTIPALHVVLITEQLTADAFAGVQQQVLLGRGAGLLAALVAGIGAYVVTRRIARPIA
                     NLTQTATRIAGGDLNQTAAIERHDEFGVLAQTFNSMTAQMRTLIGTLELRVSARTAQL
                     KASADVGRAATSILDSDQLLRQVVNLITDRFHFYYAAVFTLDDAGHYALLREATGEAG
                     RTLKQVGHKLEVGGQSMVGATIKTRTLRIALDVGHEAVRFANPLLPYTRSEIALPLLV
                     GDRVLGALDVQSEQSAAFDEASAEVLQSMADQIAIALLNAESFIRSERQTRTLELLNR
                     LSRELATATSLESISRATAQAVIDLIGPSRLFVALRGNNPDHLAVQEFLPNPEQSLGA
                     VQLVPRANTIIGQTIQTNRTFTVPDMERLADQYQDVAMYFSLGIASGVAIPMQVGEQT
                     LGVFNVGLAHKNALETDQINQLEQVAAQLAIAIENRELLQRMQSTLTELDTVNRRLIG
                     QAWEQYARASTALSGEWREGQWWQVPEPLANTLPVHGLRIPIHVRGEAIGEFNLTPTG
                     TQSEWTSDDITFAQSLIDQVGQTIENARLFEESERLALRERTINDINSRVRQTIQMDT
                     ILETAVNELARSLKATRVFARIGGHAHNLATDGNGEGDDHA"
     gene            6659..>8225
                     /locus_tag="VFF70_08770"
     CDS             6659..>8225
                     /locus_tag="VFF70_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44827.1"
                     /translation="MLKLVRALFTAPAFENAEEKTRTAGLLNIILPASLIIALSLSIL
                     TGLLGTPDPRNLAIGAGAVLVVGALWYFMRRGYVAHTSIILLIAMWFIVSMLIYLAGT
                     IRDPIASLFLIHILLAGLLIGGRAVMTVIAVDSITLLGLMSLEQARQLNQVSLYTSWP
                     EWITFVAVFSLTAVILHLTSSSIRASLEQARRTAQSLTRSNRELQLIRTSLEQRVAER
                     TEQLRVSAEVSRAITSILDPNLLIEQVVQLITNRFNFYYAAIFLIDDARQNAVLRAAT
                     GEAGRQLIERGHKLELSSPSMVGLAISTSRARIALETDTETMRFANPLLTETKSEIAL
                     PLSVGSQSFGALDVQSTLVGVFDEGTATLLQGLADQVAIALNNAQQFQISQNDARQTR
                     AIFEASRLSGSISEDLSAATTNLFRSIAEQVGFDGWSAVSFDQEQAAYTVLTSQDTII
                     QQETFISREVIPINRQPQSLLAQAIVSRRPIIIKDPQTDPRLIVLPAVVRQSMGGLIC
                     MPAILGDQVVGAIS"
BASE COUNT         1980 a         2285 c         2153 g         1807 t
ORIGIN      
        1 attcatgatt ggcatcgtgc agcagatcat cgagccgata ccggtcagcg cgtccagctc
       61 agacgaagcc attgccctgg agaacgccag attatttgcc caaacccagc aagagctcag
      121 cgaccgccaa caaaccgaac aggcagttca acgccgtgcc gatcaactcg ccgcgttgaa
      181 tcgcatcggc cgacagttgg tgcagttagc gccggcctcg gaaattttgg agacgattta
      241 tcaatcgatc ggtcagctca ttgataatcg gaatttctat ctcgcgctgc atgacgaagg
      301 cgatccggaa atccattatc caatttatac ttttgacaat gtccgcagcg aacctttcag
      361 ccgcgcgtat ggtcaagctt taaccgaaca tgttctgacc acgcagcaac ccatcttgat
      421 tccacaagat gtcaatggct atctggcgca gatcggggtc gagagtgtcg ggcgggtggc
      481 gcagtgctgg atgggtgcac ccttgctcgt cggcaaacaa gccattggcg tcattgcgct
      541 gcaagattac gagcgatcga acgcctatac tcaatcggac gtcgagctgt tgaccctcgt
      601 cgcttcacaa gcggcgatag ccttgcagaa cacccgcttg tttacgcaga ctcaagcccg
      661 ggtcgatgaa ctcgctgtgc tcaatcaaat tggccaggca gcttccggcc tcttgaatgt
      721 ggacgaattg ctcgagctca cctatcgcca agtttcgcag gtcatgcgca cagatggctt
      781 ttccgtgggt ctatatgatc ccaatcggga aatgatcagc tatccattgt gttacgcaga
      841 cagcgaaata tttcatgaac cggattcagt tatggtacct gatacccata gttaccaagt
      901 tatcacaacc ggccagccgc tcatgatcaa atattcggaa gccgatcggc aaactacgct
      961 ccagcagccc gaacccttaa tcggtgataa agtggggctg ccggcctcag tgctttttgc
     1021 gcccctaaaa atcggcgaac gcgtgctggg tttcatgaca gtccaaagtt atgaggcagg
     1081 caaacacact accgataacc acaatctgtt gatcagcatt gccaaccaag tggcaatttc
     1141 gctggaaaac gcgcggctgt tcgaacagac ccgcagccgc gccgatgaat tatcggtcct
     1201 caatgaatta gcacagcgac tgagcgcccg cttaagcact gatcaagtgc tggacgaaac
     1261 ttaccagggc attaaacgct tgatggacgc tgaaaatttc tatattggac tgtacgatca
     1321 accgaaacac caaatctatt tcatgctcaa tgtcacacaa tccgaagccg atcgtgagat
     1381 tacgcggatc tcggccgacg acggcatctc cggctatatc attcgcaatc gcaccagcgt
     1441 cttggtaggc aacggcatca atcaatgggc gcaaaagatg gggatgcgca cagtgggcca
     1501 aaacgcttta tcctggttgg gcgtgccgat gatcgtcggc aatcaagtgc tgggcgtcat
     1561 ggccgttcag aattatgaga aagctgccgc ctatagcgag catgatcgtg agctgcttgc
     1621 agtattcgcc aatcaggcgg ccaccgccat tcaaaacgca ctccttttcg aacgcaccca
     1681 acgcgacggc cagcgcgagc ggactatcaa ccgcatatcc gcgaagatcc gcaacactca
     1741 atccgtggaa cagatcttgc gcattgccac tgaagaactg cggctggcta ctcaatcagc
     1801 acggagtatt gcgcagatcg cgccagccgc agccacgccg tcagacagta gcagcaagag
     1861 cagcaactag tcaattgcga cagcccaagg atacccggct atgaaacgca acgattccgc
     1921 cccaactgca acagaccggc tgcgatccag cgtagcactt caaacacgta acggcttgat
     1981 tgcgccgatc gcattgtatt gcgaagtcgt cggcgcgcgt gatgtgcgca ttcgattggg
     2041 tgttgacgca tcgccaggtg atggcaacgg aaggcagaac gatcatgcct aacctactca
     2101 ctcgctggct aacaaccgag acgcgccgtt atgttgccct cggcgcagct tttggtttag
     2161 tgtttccgct aatcgctaca ctggtcacct tgattctccg ccaactgccc atcagtgtga
     2221 gtaatatgtg ggccgttcaa actactgatc ccttattgtg gatcatcgat accgcgcctt
     2281 ttttcttggg cgcgttctcc ggtctagctg gacgtcggca agatgcttta caatcaacca
     2341 atcaacgcct gcgcgaggcg acacacgcgc ttgaaaaaca ggagcagcga caagctgatg
     2401 aattagggag acaggctgct caactgaaag ccagcgccga tgttagccgc gccgccgcct
     2461 caacactcga tcgtgaagag ctgctgacaa agatcaccga gctgatcgta gagcgatttg
     2521 gcttttacta tgcagcagtc tttctactgg acgcaagcgg cgagaatgtt gtcttgcata
     2581 aagctaccgg cgaagccggc cgcatcctca aagagcgcgg tcataagctg cctgtggata
     2641 acgcctccat ggtcgggtcg gctatcacca cgcgcaaacc gcgcatcgct ttggatgtcg
     2701 gtaaagagcg cgtccgcttt gccaatccac tgctaccgaa tacacgatcg gaaattgcgc
     2761 tgccgttggt ggtaggcgat cacgctttag gggccttaga cgtgcaatca atccaagaag
     2821 ccgcatttga tgatgccaat gcggcggtat tgcaatcaat ggccgatcta attgctgtag
     2881 cactcaacac agccacgtca ttcagtcagg cgcgaactga gacccaacgc gctaaacaaa
     2941 ttgctacact gttaggcagc acgctggaat tgaccctgca cagcgatcga cagacagtgt
     3001 atgaccgcat cgttcgaatt gggatggatt tggttaatgc cgacggcgcg ggtctggcgc
     3061 tgcccatctc agacacggaa ctcgaaatga aaacctcaat cgaagtcggg tctgatccgc
     3121 agcaaatgac tggccgccgg ttgaaaataa atgaaagcat ttcaggccga gtcttcgcca
     3181 caaaacaaac actgcgcatc gataactatc caacttggag cggtcgttca caggctttca
     3241 ccgatgcgcc ttctcaggca atcttaggcg tgccactcgt ctggcaggat cgagtcctgg
     3301 ccgtattgac cattactcat tcgcagccca atcaaccctt caccgaagac gatgagtttt
     3361 caatcaagct gctggctgca caaggtgctg ccactttagc caatgttgat ctgcgcgaaa
     3421 atcaagcgca aactttgaat gaattaaatg ccttgaactt gcgcctgacc cgtgagacat
     3481 ggacggcatt ctcgaaacaa ctcaagcgcg aaggcgtgga ttggatcggc atgggtgaac
     3541 caatcgggcc tgatcgacag ccggaagtca gcgaagcgct gagcagcgga cgaatcgcca
     3601 cgaattcaga atcggatcaa aaccaaaccg cggtggctat cccgatcgtt ttacgcggtg
     3661 tgccgatcgg tgtgatgcgg ctgcgagtac cgtcggaaaa ttggtcaagc agcacagcca
     3721 gcacattaac atcaatcgcc gggcatattg cgcaagccat tgaaaacgcg cgtctactgc
     3781 aagtgactga ggaacgcttt gcccgcgagc gggacttgag cgaatcgact gacaagattc
     3841 ggcgcaaaac tgatgtcgag cagatcttgc agaccgccgc cgaagagtta gcccgccatt
     3901 tgcaagccac gcgcgtagcc gtaagtattg gtccacagcc gagtgccgag agcgatcgat
     3961 tgtgaggaat tgatgagtca acttccaaaa cttcctcaat ccaataaacc attcaccgcc
     4021 accggcggta tcggccgcac tctgttgctg tcgttcttaa tcttgacgct ggtgccgctc
     4081 acactggtac tcgttatcgg cacgaacatc agttttcaaa cggcgcaggc cgatgccttt
     4141 gcccggctga gtggcgtgtc agagataaaa gttcaagcga ttgaggattg gctcggagag
     4201 cgccagaata atctgctgga cattctaggt atcgctggga ccccccagct actgcaagat
     4261 attttgcgta atccgaataa caatactgcg gctgcggcac ttgcgactaa tttatccgac
     4321 gaagttcgag agcatcaagt ttttgcgcgc tttatcgtgc tcgatgcagc cgggcacagc
     4381 ttaatcagta ccgatcccgg gttggttggc caatcctatt caagcgaaac gtatttcaca
     4441 cgcggcctgc tcggtccgtt catcacttca gcacagaacg acatgaccgg acgttacgat
     4501 acaatcgtcg tggccgctcc ggtggtcaat gcggccaaac aaaccgtcgg ggtgttagtc
     4561 gctgaagcga atatcgatca gctgtctcgc atcgtattaa cgagtctggg gacgaatcgt
     4621 acggatgatt catatttggt cggccccgat cgccgcttct tgacgcaatc acactatctg
     4681 cacacagaaa attccgggga tattgtagaa acggaggccg tcatcgcagc tctaaatgat
     4741 ccagcggcga tcggtcaggc tacctacaac aactatatta atcagccagt cctcggtgtg
     4801 taccgtacga ttccggcctt gcatgtggtt ttgattactg aacaattaac ggcagatgcg
     4861 ttcgccggag tacaacagca agttttgttg ggtcgtggag ccgggttgct ggcggcgctg
     4921 gtggcgggca ttggcgcgta tgttgtcacc cggcgcattg cgcgccccat tgccaatttg
     4981 actcaaacag caacgcgaat tgccggcggc gatctaaatc aaacggccgc catcgaacgg
     5041 cacgatgaat tcggcgtgtt ggcgcaaaca ttcaactcga tgacagcgca aatgcgcaca
     5101 ttgatcggca cgttggaact gcgcgttagc gcacgcaccg cccagttgaa agcgtcagcc
     5161 gatgtaggtc gcgcggcgac atcgatctta gatagtgatc aactcttgcg ccaagtagtc
     5221 aatctcatta ccgatcgatt tcatttctac tacgcggccg tgttcacttt ggacgacgcg
     5281 ggccattacg cgctattgcg cgaagcaacc ggcgaagccg gccgcacact taaacaagtc
     5341 ggtcacaaat tagaagtcgg cgggcagtcg atggtgggcg caaccattaa aacacgaacg
     5401 ttgcgcatcg cgctggatgt cggccacgaa gctgtccgtt ttgcgaatcc actgctgccc
     5461 tacacccgat ccgagattgc cttgcccctg ctcgtcggcg atcgggtgct gggcgcgctc
     5521 gatgttcagt cagaacaatc agccgccttc gatgaagcca gcgccgaagt gctgcaatcg
     5581 atggccgatc aaattgcgat tgcattgctc aatgcagagt cattcatccg atctgaacgg
     5641 caaacacgta cactggaact gctcaatcga ttaagccgcg agttggccac ggcgacatcg
     5701 ctggagtcga tcagccgggc aaccgcgcaa gctgtaatcg atcttatcgg tcccagccga
     5761 ttgtttgtcg cgctgcgtgg caataatcct gatcacctgg ccgttcaaga atttctaccc
     5821 aaccctgaac agtcgttggg cgctgtccag ttggtccccc gcgccaatac catcatcggc
     5881 caaacgatac agaccaaccg gacatttact gtgccagata tggaacgttt ggccgatcaa
     5941 tatcaagatg tggcgatgta tttcagtttg ggtattgcat ccggcgtagc gattccgatg
     6001 caagtgggtg aacaaacatt gggtgttttt aacgtcggtt tggcacacaa gaacgcctta
     6061 gagaccgacc agattaatca gctagagcaa gtggctgctc aactggccat cgcgatcgaa
     6121 aaccgtgaac tgctgcagcg aatgcaatcg acactgactg aattggatac ggttaaccgc
     6181 cgcttgatcg gccaggcgtg ggaacaatat gcacgcgcct ccactgctct ctccggcgaa
     6241 tggcgtgagg gacagtggtg gcaagtgcct gagccccttg ccaatacgct gccggtacat
     6301 ggcctgcgaa ttccgattca tgtgcgcggt gaggcgattg gagaattcaa cttgaccccg
     6361 accggcactc aaagcgaatg gacttcagat gacattacct ttgcgcaatc gctgatcgat
     6421 caggtgggac aaacgatcga gaatgcacgc ttgtttgagg agtctgagcg actagccctg
     6481 cgcgagcgga cgatcaacga tatcaatagc cgggtgcggc aaactattca gatggacaca
     6541 attctggaaa ccgccgtcaa tgaactagcc cgatcgctga aggcgactcg cgtctttgcg
     6601 cgcattggcg gccacgctca caatctcgcg actgacggca acggggaagg tgacgatcat
     6661 gcttaagctg gtccgcgcct tattcactgc acctgccttt gaaaatgctg aagagaaaac
     6721 tcgaacagcc ggattgctga atatcattct gcctgccagc ctaatcatcg cgctgagctt
     6781 atcgatcttg accggtttgc tgggcacgcc agatcctcga aatctcgcga tcggcgccgg
     6841 agcggtattg gtggtgggag ctctatggta tttcatgcgg cgcggctacg tggctcacac
     6901 cagcatcata ctcctgatcg ccatgtggtt cattgtatcg atgttgattt atttagctgg
     6961 gaccattcgc gatcctattg cctcgctatt tctgatacat attctattag ccggcctgct
     7021 gatcggcggt cgcgcagtga tgaccgttat cgcggtcgac tccatcaccc tgctcggctt
     7081 gatgagcttg gagcaggcca gacaattgaa ccaagtttct ctgtacacca gctggccgga
     7141 gtggattacc tttgtagcag tcttcagcct gacggctgta attctacact tgacttcaag
     7201 cagtatccgc gcatcgctgg aacaagcgcg gcgcaccgcg caatcattaa ctcgcagcaa
     7261 ccgcgagtta caattgatcc gcacttcgct ggagcagcgc gtagcggagc ggaccgaaca
     7321 gctccgcgtc agcgccgaag tcagccgcgc catcacatcc atcttggatc caaacttgct
     7381 cattgagcaa gtcgtacagc tcatcaccaa tcgctttaac ttttactacg cggccatctt
     7441 tctgatcgat gacgcgagac aaaatgctgt actgcgcgct gcaacgggcg aagccggacg
     7501 acagttgata gagcgcggcc acaaattgga actgagcagc ccgtcgatgg tgggtctggc
     7561 catcagcacc agccgagcac gcatcgcgct ggaaaccgat accgaaacga tgcggttcgc
     7621 caatccactg ctcacggaga ccaaatctga aattgcactg ccgctcagtg tcggttcgca
     7681 atcttttggc gcacttgatg tgcaatcgac gctggtcggt gtgttcgatg aagggacggc
     7741 tacgctattg caaggcttgg ctgatcaggt cgccattgcg ctgaataacg cccaacaatt
     7801 ccaaatctcc cagaacgatg cccgtcagac tcgcgccata tttgaagcca gccgtttatc
     7861 aggttccatc agcgaagatt taagtgccgc cacgaccaat ctgttccgat cgattgccga
     7921 acaagtcggt ttcgacggct ggtcggccgt ctcattcgat caagagcaag ctgcttacac
     7981 agttctgacc agccaagata cgatcattca gcaggaaaca ttcatcagcc gcgaagtcat
     8041 acccatcaat cgacagccac aatcattgct ggcccaggcc atcgtcagtc gtcggccgat
     8101 catcatcaaa gatccacaga ctgatccgcg gttgatcgtt ctgccagctg ttgttcggca
     8161 atccatgggc ggcctgatct gtatgccagc cattttgggt gatcaggttg ttggcgcgat
     8221 cagtt
//
LOCUS       DASRTJ010000430         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_64858,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000430 DASRTJ010000000
VERSION     DASRTJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64858"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFF70_08775"
     CDS             <1..197
                     /locus_tag="VFF70_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44828.1"
                     /translation="PPYQFFFDNIQEPGDQFEYVAAKCRSRPTTLTVKSADGQSSAQV
                     VSLIAPYCPVTPEPTRGGKP"
     gene            411..1127
                     /locus_tag="VFF70_08780"
     CDS             411..1127
                     /locus_tag="VFF70_08780"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY44829.1"
                     /translation="MAANGSVTLVMRQGPKPNQVFELTKDVYTAGREAGNDIAIEDTQ
                     ISRHHARLSRQGNSFVVEDLGSTNGTFVNGRRVTGPVLLSNGDMLGFADTIVLAVQIQ
                     SPMSDATVVSHGAGATGVGSSTAPSFAPPPPPPARPAPAPQQPVYVPPPQPVYAPPPQ
                     PVYAPPPQPVYAPPPQQPMMQQPMMSQPAKSNTGRYVLIGLGCLVLLVIVGVIGVVAW
                     SFIDCGSFKSVFGGLIGISC"
     gene            complement(1285..2349)
                     /locus_tag="VFF70_08785"
     CDS             complement(1285..2349)
                     /locus_tag="VFF70_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44830.1"
                     /translation="MLLFSLTQSGVRAGLGWGLMSVTQVMDRLYQINVPTPFKVGPVN
                     CYLSIGDPITLIDTGPLHADTRAKLNAGLKEIGVTRSDIQRIIVTHAHSDHYGLAAEI
                     VRDSNAEVWTHRYNRSTLEAHEADRQRRSEFYWQIMIEAGVPDEERQRLADSRHGGER
                     FAESVKIDRTIDEGDKIEFAHKHWQVYHTPGHAGGLIVLFDPVSRVLLSNDHLLRDIS
                     SNPVVEPAPEGGPRPHRLVEYIYHLQRMIELKPVVAWTGHGDPVRDVAKLVKQRLAFH
                     SRRAEKIIDLLGAADRHLHRNAEQCGSAYALAEPLFGRLNGIDSFLALSETIGHLEWL
                     QEQGRVTSIEREGVVYWRRL"
     gene            complement(2405..2869)
                     /locus_tag="VFF70_08790"
     CDS             complement(2405..2869)
                     /locus_tag="VFF70_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZY44831.1"
                     /translation="MTDYAPRGLYYEDMEVGFKVVTAGRTISEADIMAFAGLSGDWNA
                     IHIDAEYAKTAGFGERVAHGLLVLSIASGLAMQMGFLDRTVEAFTALDWKFRAPTKIG
                     DTIHLEAEVKEKKAVPGGMGGFVIFNMTIKNQRDEKVQRGTWMVVVKSKPKE"
     gene            complement(2929..3162)
                     /locus_tag="VFF70_08795"
     CDS             complement(2929..3162)
                     /locus_tag="VFF70_08795"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HZY44832.1"
                     /translation="MIEQLRAMLKRGGTLTLDQIAHELDTTPEMIVQLLDHLERNGLL
                     RQISRDCQATCDGCYLAQACARSEGQRIWSSVN"
     gene            complement(3197..>4024)
                     /locus_tag="VFF70_08800"
     CDS             complement(3197..>4024)
                     /locus_tag="VFF70_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZY44833.1"
                     /translation="GLHGKSFLPLFLGFGCNVPAVMGTRVIESEKSRLLSILIAPLVP
                     CTARMAVVAFITPAFFGANAPLVAFGLVMLNLVVLAVVGVIVNRTIFKSGHSAFIMEL
                     PQYHWPQARSIALIVWQRTIGFIKQAGTAILIMSLIIWALAYFPNGQLETSWLGGLGR
                     TLSPIGGLLGLDWKLLIALLASFVAKENSIAILGVLFSAGGNHTQLASTLATLITPIA
                     ALSFLVVSMLFIPCASTVATIRKETQSWRWTLFTIGLLACVSFGVGIAVYQIARVLT"
BASE COUNT          925 a         1232 c         1014 g          853 t
ORIGIN      
        1 aaccgccgta tcaattcttc tttgataata tacaagaacc cggcgatcag tttgaatatg
       61 tggcggccaa atgtcgaagt agaccaacca cactcacagt gaaatcggct gatggtcaat
      121 catccgccca agtcgtgtcg ctcattgcac cctattgccc ggtgacgccg gaaccaacgc
      181 gcggcggcaa gccgtaagat cgatcgggct ggtggatcga tcgagcaaaa ttgtaaaacc
      241 tggcgagggt ttcagctaca gtgcatgact cagcagccca accctcgcca ggttgatcgc
      301 tcaacccgat cggttttcca gctcatcgat cggtttgaca tccaatcaaa atctgaatat
      361 actgccagcg ttcaggcggt tacagacatt catcatcagg aggcagcaag atggcagcta
      421 acggttcagt gactttagtc atgcgacaag ggcctaagcc caatcaagtt tttgaattga
      481 cgaaagatgt ttacacggcc gggcgcgaag caggcaacga cattgcgatc gaagacacgc
      541 aaatctcgcg gcatcacgcg cgcttatcgc ggcaaggcaa ttccttcgtt gtcgaagatc
      601 tgggcagcac caacggcacg tttgtcaacg gccgccgtgt aaccggtccg gtcttgctgt
      661 ccaatggcga catgctcggc ttcgccgaca cgatagtgtt agccgttcaa atccaaagtc
      721 ccatgtccga tgccaccgtt gttagtcacg gcgcgggtgc cacgggtgtt ggttcgtcta
      781 ctgcgccgtc atttgcgccg ccgccacctc caccggcccg ccctgctccg gcaccacaac
      841 agccggtcta tgtgccacct ccgcagccgg tctatgcgcc accgccgcaa ccggtctatg
      901 cgccgccgcc ccagccggtc tatgcgccgc cgccccaaca gcccatgatg cagcagccca
      961 tgatgagcca gcccgccaag agcaacaccg gccgctatgt cttgataggc ttgggctgtc
     1021 tggtcttgct agtgatcgtg ggtgtcatcg gcgtcgtcgc gtggtcgttc atcgactgcg
     1081 gctcattcaa atccgtcttc ggcggactga tcggcatcag ctgctaagcg actacttctc
     1141 aaacgaaaac tcctcggctc atcaccgggg agttttttgt ttgttgcaac cgtcattgcg
     1201 aggagcgctc tacgcgacga agcaatctct cttttataag cgatgagatt gcttcttcac
     1261 ctcgttcctc gcgatgacgc actctcacaa tcgtcgccaa tacacaacgc cctcacgctc
     1321 gatcgatgtg acacgcccct gctcctgcaa ccactctaaa tgcccgatcg tttccgataa
     1381 ggccaggaac gaatcgatcc cgtttaagcg gccgaacaag ggctcggcca gtgcgtaagc
     1441 cgacccgcac tgctccgcat tgcggtgcaa gtgtcgatcc gctgcgccca acagatcgat
     1501 aatcttctcg gctcgacgcg agtgaaacgc gaggcgctgt ttcaccaact tggctacgtc
     1561 gcgaactgga tcgccgtggc cggtccaagc cacaaccggc tttagctcga tcatgcgctg
     1621 caggtgataa atgtattcca ccaatcgatg cggacgcggc ccaccctcgg gcgcgggttc
     1681 aaccaccgga ttgctgctaa tgtcgcgcag caaatgatca ttagagagca ggacccgcga
     1741 aacgggatcg aacaatacga ttaagccgcc cgcgtgaccg ggcgtgtggt acacctgcca
     1801 gtgtttatgt gcaaactcga tcttatcacc ttcatcgatc gttcgatcga tcttaacgga
     1861 ctcggcaaag cgttcgccgc cgtgccgact atcagccagg cgttgacgct cttcgtccgg
     1921 cacacccgct tcgatcatga tctgccaata gaactcggat cgtcgctggc gatcggcttc
     1981 atgcgcttct aaagtcgatc ggttgtagcg atgcgtccac acttccgcgt tcgagtcgcg
     2041 cacgatctca gcggccagtc cataatgatc tgaatgggca tgcgtcacaa taatccgctg
     2101 aatatcgctg cgcgttacac caatctcttt caaacctgca ttgagtttag cgcgcgtatc
     2161 ggcgtgcagc gggccggtat cgatcaacgt gatcggatcg ccgatcgaga gataacaatt
     2221 caccggcccc actttgaagg gtgtcggcac attgatttga tagagacgat ccatcacttg
     2281 cgtgactgac attaaacccc agcctagccc cgctcgcacg ccgctttgcg tgagagagaa
     2341 cagcaacatc aaacaaaagg ccgctcaatc gatcaagcgg cctctaatat ctaacttcca
     2401 acttctattc cttcggtttg ctcttgacca caaccatcca cgtaccgcgc tggactttct
     2461 catcacgctg gttttttatg gtcatgttga atatgacgaa gccacccatg ccgccgggca
     2521 cagccttctt ctctttgact tcggcctcaa ggtggattgt atcaccgatc ttcgtcgggg
     2581 cgcggaattt ccaatcgagg gccgtgaaag cctccacggt tcgatcgaga aagcccatct
     2641 gcatcgccag gccggacgcg atcgatagca ccaacaggcc gtgcgccacg cgctcgccaa
     2701 aacctgctgt cttggcatac tcggcgtcga tgtggatggc attccaatcg ccgctcaatc
     2761 cggcgaaggc cataatgtct gcttcgctga tcgtgcggcc cgctgtcact actttgaagc
     2821 cgacctccat atcctcgtaa tacaagccgc gcggtgcgta gtctgtcatg aatgactcct
     2881 catcccggtc tcgcgccgat tgtaatagcg aactcgcgat tggtcaattc aattgacact
     2941 tgaccaaata cgctgccctt ctgatcgagc gcaggcttgc gcaagataac agccgtcgca
     3001 agttgcttga caatccctac tgatctgccg caacaaacca ttgcgctcaa gatgatcgag
     3061 caactgaacg atcatttccg gcgtggtgtc caactcatgc gcaatctgat caagcgtgag
     3121 tgtgccaccg cgcttgagca tcgccctcaa ttgttcaatc atgttcggct cctgtgggac
     3181 aacttggtaa attgtcctac gtcaaaactc gcgcaatttg atagaccgcg ataccaaccc
     3241 cgaatgacac gcacgccagc agaccgatcg taaacagtgt ccacctccac gattgcgttt
     3301 cttttcgaat cgtcgccacg gttgaggcgc acggaatgaa cagcatcgac acgacgagaa
     3361 aactcagcgc ggcgatcggc gtgatcaatg ttgccagtgt cgaagccaac tgcgtatgat
     3421 tgccgcccgc gctgaataac acgcccagaa ttgcgatcga attttctttg gcgacgaacg
     3481 aagccagcag cgcgatcaac aacttccaat ccaagcccag cagtccgccg atcggcgata
     3541 gcgtgcgacc gagaccgccc agccacgaag tctcgagctg gccgttagga aaataagcca
     3601 gcgcccaaat gatcagcgac atgatcaaga tcgccgtgcc cgcttgtttg ataaaaccga
     3661 tcgtgcgctg ccagacgatc aatgcgatcg atcgagcctg gggccaatga tactgcggca
     3721 gttccatgat gaaggccgag tgtccactct tgaagatcgt tcgattgacg atcacgccga
     3781 cgacggccag caccacgaga ttcagcatca ctaaaccaaa cgccaccagc ggcgcattcg
     3841 caccgaagaa ggcgggagta atgaacgcaa ccaccgccat ccgcgccgtg cacggcacca
     3901 acggcgcgat caagatcgaa agcagtcgcg atttctctga ttcgatcacc cgcgtgccca
     3961 tcactgctgg aacattacag ccaaagccga ggaacaacgg caagaatgat ttgccgtgca
     4021 agcc
//
LOCUS       DASRTJ010000431         9858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_329883,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000431 DASRTJ010000000
VERSION     DASRTJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329883"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFF70_08805"
     CDS             complement(<1..209)
                     /locus_tag="VFF70_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44834.1"
                     /translation="MSEVRADLLPERELSPAEIEALVEFLRQIPLFAELRTTDLEALV
                     TIAHSEFFAAGGELYHQSEDDNTLY"
     gene            complement(1266..2651)
                     /gene="dnaA"
                     /locus_tag="VFF70_08810"
     CDS             complement(1266..2651)
                     /gene="dnaA"
                     /locus_tag="VFF70_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZY44835.1"
                     /translation="MKSDQIWQAALGELQLQMTKATFDTWVKPTSVISYEDGSFVVGV
                     PNAYAQDWLQTRLLSTIKRILTGIAGQSVEVKFIVWIKEETAETVPLLEGLEAEPPIS
                     IMASRSNGNYGNGRSIGGLNPRYVFDTFVVGPSNRLAHAACVAVAERPAAQYNPLFLY
                     GGVGLGKTHLLHAIGHEGVAKNLQVLYVSSEEFTNDMINAIRTQNQAAFRDKYRTIDV
                     LLIDDIQFIAGKESTQEEFFHTFNTLHGNGKQIVMTSDRPPKSMITLEERLRSRFEWG
                     LIADIQQPDLETRTAILRDKAEHLAAAVPSDVIDFIARQVQSNIRELEGALNRVVAYA
                     RLRGNPLTVEIASAALADLMSRPSTVTIDEILSTVANYYSISMDDLLGRGRNRELVHP
                     RQVAMYLAREELQVTLPQIGEALGGRDHTTVMYGVEKVTQAIDSDDAVRREILALRER
                     LYNRNTVLDRT"
     gene            3123..4160
                     /gene="rfbB"
                     /locus_tag="VFF70_08815"
     CDS             3123..4160
                     /gene="rfbB"
                     /locus_tag="VFF70_08815"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZY44836.1"
                     /translation="MKNLLITGGAGFIGSNYVRYVLENHSNLNAIVFDKLTYAGNLDN
                     LRDVNEKFRARYHFVQGDICDAESVAAAMRDHHIDTIVNFAAESHVDRSLLTPDAFVK
                     TDVNGTYVLLEAAKIFQVERYHQVSTDEVYGDIPTGSSRETDLLNPRSPYSASKAGAD
                     LMVNAYHVSFGLPTTITRGSNTYGPYQYPEKLLPLFITNALEDQLLPIYGDGLQVRDW
                     LYVLDHCSGIDLVLRRGTIGETYNLGGENEQHNIDVIRLLLKMMHKPEALLRRVTDRP
                     GHDRRYSLDVSKLHALGFEQSATFEQELEATVKWYTENEWWWRKIKTGEYLEYYKQQY
                     GRRLAESEKIN"
     gene            complement(4417..6066)
                     /gene="hutU"
                     /locus_tag="VFF70_08820"
     CDS             complement(4417..6066)
                     /gene="hutU"
                     /locus_tag="VFF70_08820"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZY44837.1"
                     /translation="MSRIIRAPRGTTITCKGWQQEAVMRMLMNNLDPDVAEKPDDLIV
                     YGGRGRAARSWEAYDAIIATLKELEDDETLLVQSGKPVAVFKTHRDAPRVLIANSNIV
                     PHWATQEQFDQYEREGLMVYGQMTAGSWIYIGSQGILQGTYETLAELARQQNWTEGLK
                     GKFVLTAGLGGMGGAQPLAITMNGGVGLVVEVDLARAQRRLETKYVDVVVDTLEEAMT
                     LVQDAKDQGQGLSIGLIANAADIFPELVLRGIVPDVVTDQTPAHDPLYYMPHGMSIED
                     AAKLRSTDPAEFTRRSMHSMARQVQAMLDFQKRGSIVFDYGNNLRQRAFDAGVQNAFD
                     YPGFVPAFIRPLFCEGKGPFRWVALSGDEKDLFRTDEAILELFPEDKSLERWIRLAQK
                     RVAFQGLPARICWLGYGERAKAGLKFNELVATGEVSAPIVIGRDHLDAGSVASPRRET
                     EAMLDGSDAISDWPILNAMINAVGGATWVSFHHGGGVGIGYSQHAGQVIVADGTPEAA
                     KRLERVLTTDPGMGVVRHADAGYPAAIEFAKKTGIKMPMLK"
     gene            6299..7054
                     /locus_tag="VFF70_08825"
     CDS             6299..7054
                     /locus_tag="VFF70_08825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY44838.1"
                     /translation="MTDYAPFAALYDLFYADFEDDIEMYRGFADRTGGAILEIGSGTG
                     RVSIALAGEEHHVTGVELSDEMISLAQKKIEASGLSKQVNLIQAEMRRFTLDDHFGLA
                     IVPLNTFLHNLTLDDQLSTLNSIKKHLTPNGLLVLDCFLPDPTNADDHRLILQRSVID
                     RATGTAAQLFLSRTTDWANQRQDITYFVDRSDRSGQVQRNVFNTSFRFIYRNEMQLLL
                     KQGGFDLKEVYGSYELDPFDASSDKMIVVATPT"
     gene            7118..7315
                     /locus_tag="VFF70_08830"
                     /pseudo
     CDS             7118..7315
                     /locus_tag="VFF70_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(7344..9050)
                     /locus_tag="VFF70_08835"
     CDS             complement(7344..9050)
                     /locus_tag="VFF70_08835"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZY44839.1"
                     /translation="MTNTATDQLRQLLNDEWEYRLKEDPLFATDVGDHRYDDRLPAVT
                     EADYQRRLDQARNFLDRSGEIDRAQLSRSDQLNLDIFRRVKLDDVAELEYRSYLMPID
                     RMGGFHTNFAELPNFLALESARDYENYIARLRGFKEYMTQYIELMRVGIRSGYMPPAV
                     ALNSVADSIQPHLIEDAARSLLFAPFNKFPDNIHAAQQKDFTDRARKAIETSVTPAYR
                     SLMQFVTEEYLPAARSDIAASSLPNGKAFYEHRVRLFTSLNLTPQQVHDTGLSEVKRI
                     RAEMETIIKKVEFNGSFKDFVEFLRTDPRFYVSTPDALMKEVSFILKKMDGELPKLFK
                     TMPRMPYGIRPVPDYAAPSTTTAYYFQPAGDGTRAGFYYVNTYNLKARPLYELEALSL
                     HEAVPGHHLQIALQQELENIPIFRRFTGFTAFVEGWGLYSERLGLEVGFYQDPYNDFG
                     RLTYEMWRACRLVVDTGLHYLGWPRHRAIDLMADNTALTLLNISNEVDRYISMPGQAV
                     AYKTGELKIRELRSIAERELGAKFDVREFHDVLLRDGAVPLDVLETIIKSWIEEAKRR
                     EK"
     gene            complement(9061..9345)
                     /locus_tag="VFF70_08840"
     CDS             complement(9061..9345)
                     /locus_tag="VFF70_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44840.1"
                     /translation="MQQTFNLGPEYDHKMLQALFDALTAEGAQTIDQRTAEGAGSQEI
                     GYWKFQLDRGTVEVNAETYLGISLSGPVDLVARLTPIIQDKILSAFPGRE"
     gene            complement(9353..9544)
                     /locus_tag="VFF70_08845"
     CDS             complement(9353..9544)
                     /locus_tag="VFF70_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44841.1"
                     /translation="MARTFNDDEICKILIGYLDHCTYSAQELTSILKQLRAEGLAIRK
                     DDRWFYQPKHSRHDRSFIF"
     gene            complement(9625..>9858)
                     /locus_tag="VFF70_08850"
     CDS             complement(9625..>9858)
                     /locus_tag="VFF70_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZY44842.1"
                     /translation="VYQYATGIAGAHALAKRILSGEKGAANDYINFLKAGDSMYSLDA
                     LRLAGVDLSKPQAVDAAFAVLSGMVDKLESLLG"
BASE COUNT         2328 a         2628 c         2539 g         2363 t
ORIGIN      
        1 atatataagg tgttgtcgtc ctcgctttgg tgataaagtt cgccgcctgc agcaaagaac
       61 tcactgtgcg caatggtgac caacgcctcc aagtcagttg ttcgcaattc agcaaataaa
      121 gggatctggc gtaaaaactc caccagcgcc tcgatctctg cggggcttag ttctcgttcg
      181 ggtaaaagat cggctcgaac ttcactcatc gcatttatct tctattctga aagattgtat
      241 cataagtaaa gcgcacctaa aagctgcgaa gcagtcgaaa tgcattgttc tcaaggctgg
      301 actgtggccg agcaacacta ttaagcagat ctgtatcgag aatgaaatat tgtctacttt
      361 ttgtatgtgt acggagtctc caatgaccgc ccctctactg gccgtacagt aatattcttc
      421 gcataaaaac ccgatcgggt ctagttgaac ccgttaaagt ttatcgattg ctggaggatc
      481 tgttcactca ctgcctgagt tcaggccacc agtctacgtc gaagaactgc tgattgtcgg
      541 tgtcgacgat aaaggcatca accgcgaggt caccctaact tatcgatcgg ttactcagcc
      601 gcattctgcg cagcgctaaa tccactaacc gtttttccag ctgatcattg aagttttagt
      661 atatccttag caaatgtgtt gaaggatata tattatttag tagtagtagt aggcagtgtg
      721 aatttgtgga gggccacaaa ataacggtac atgtagccgc ccttagctga tcaatctgta
      781 taggtaggcc tttagcgttc taagttgatc ggtcgaattg tgggcggtct gtgtatggat
      841 gcaaccagac tttcaggttt tatatgtgga aatctatccc caaccagata ttgggcatcc
      901 acttaattcg accgccacat atagggcata agaataaatt cttatgtact aaatttaaaa
      961 gttatcccca aaagcatacc cttatccaca ataattcgtc acttttccac aaattcaaca
     1021 agccaaatta ttaacatggg ttttatgtcc ccctatatgt agacgctgaa aaactcggtt
     1081 tttacagctt atccacaagt ggagggattt atccacagat agatggttct tttccataag
     1141 ttacgtcatc taaatttata aagaatgaag gggcaagcgc tttgcttacc ccttcattca
     1201 cgtggtcttt caggtcaaaa gaaggaccac acgcgttatg agccaacacc acgtggcgaa
     1261 gtgatttaag tgcgatccaa aacagtgttg cggttataca gccgctcgcg cagcgcgagt
     1321 atttcgcggc gaaccgcatc atcactatcg atcgcttggg tgactttctc tacaccgtac
     1381 atgaccgtcg tgtgatcgcg gccccctaag gcctcgccga tctgcggcag ggtcacttga
     1441 agttcctcgc gggcaagata cattgccact tgacgcggat gcaccagttc tctattgcgg
     1501 ccacggccca agagatcatc catagaaata ctgtagtagt tcgcgactgt cgatagaatc
     1561 tcgtcgatgg tgacggtcga tggccgtgac atcaaatcag ccagcgccgc cgaggcgatc
     1621 tcgacggtca gcgggttgcc gcgcagccgg gcatacgcga ccacgcgatt tagcgcgcct
     1681 tccaactcgc ggatgttgct ttgcacctgc cgcgcaataa agtcaatcac atcgctgggc
     1741 acggccgcgg ccaggtgctc ggctttgtcg cgcaggatcg ccgttcgggt ttccaaatcc
     1801 ggctgttgaa tatcggcaat cagtccccat tcgaaccggc tgcgcaagcg ctcttccaaa
     1861 gtgatcatcg acttcggcgg gcgatcgctg gtcatgacaa tctgcttgcc gttgccgtgc
     1921 agcgtattga acgtatggaa gaattcttcc tgcgttgatt ctttgccggc gatgaattga
     1981 atatcgtcga tcaacagcac atcgatagtg cgatacttgt cgcggaaggc cgcttgattc
     2041 tgcgtgcgga tggcgttgat catatcgttg gtgaattctt ccgaggagac atacaacact
     2101 tgcagattct tggcgacacc ctcgtgaccg atagcgtgca gcaagtgcgt cttgcctaaa
     2161 cccacgccgc catacaggaa caacggattg tattgagcgg cgggccgctc ggccacagcc
     2221 acacaggccg cgtgagctaa tcggttgctg ggaccaacca caaatgtatc gaacacgtag
     2281 cgtggattga gtccgccgat cgatcgaccg ttgccatagt tgccgtttga ccttgaagcc
     2341 atgattgaaa tcggcggctc cgcttcaagt ccctcgagta acggcacagt ctcggcggtt
     2401 tcttctttga tccatacaat gaattttact tcaacgctct ggccggcgat accggtgagg
     2461 atgcgcttga tggtggacag caagcgcgtt tgcagccagt cttgtgcgta cgcattcggc
     2521 acgccaacga caaacgagcc gtcttcatac gaaatcacgg atgtcggttt gacccacgta
     2581 tcaaacgtgg ccttggtcat ttgcagttgt agttcaccga gtgcggcttg ccagatctga
     2641 tcagatttca tatcgatgcg ctccccccag ctgatctttt tgatcaaagg ggtgttgaga
     2701 taacactgcc tccaacggcg cgggccaacg ctggatcagt attcaattga tgttaagcag
     2761 tgtacagctc gatgaccgta gacggaaaca ggcagaacga gggagtgaaa cgctgccagc
     2821 ctgttgagcc aaaattttat tagggcgaac tcgcttcctt taaagaaacg gtcggacaag
     2881 aatttgattt ccttccgact cacaagcacc attctatcaa atgctgatcg atttggcaat
     2941 aggcaaacct taaaacgatc aaagctttaa ggatcgaaaa gctgaacccg atcgtttgat
     3001 ctgatcccgc gttatttttc gctttccaca cctcatggta tcattctccc cgttgataca
     3061 gtgttatcca cgattatcca ctgtctatcc acaaagtaat ccacacacat tgaggagaac
     3121 tcttgaagaa cctattgatc acagggggcg cgggcttcat tggctcaaat tacgtccggt
     3181 atgttctgga gaaccattca aatctgaatg cgatcgtttt cgataagctt acttatgccg
     3241 gcaacctcga caacctgcgc gacgtcaatg aaaaatttcg agcgcggtac cattttgtcc
     3301 agggcgacat ctgtgatgct gaatccgtcg cggcagctat gcgcgatcat cacatcgata
     3361 caatcgtcaa cttcgcggcc gaatcgcacg tcgatcgatc gctgctcaca cccgatgcat
     3421 ttgtgaagac ggacgtgaac gggacttacg tcctgcttga agcggcgaaa atttttcagg
     3481 ttgaacgcta tcatcaagtt tcaaccgatg aggtctacgg cgacatcccg accggttcat
     3541 cgcgcgaaac ggacctgctg aatccgcgca gtccatactc ggccagcaaa gctggcgccg
     3601 atttaatggt gaatgcctat cacgtttcct ttggactgcc aacgacgatc acacgcggat
     3661 cgaatacgta tggcccgtat caatatcctg agaagttgct gccgctcttc atcaccaacg
     3721 cgctcgaaga tcaactgctg ccgatttatg gcgatggtct gcaagtgcgc gattggctgt
     3781 atgtgctgga tcactgctcc ggtatcgatc tggttttgcg gcgcggcacg atcggcgaaa
     3841 cgtacaacct cggcggtgag aacgaacagc acaacatcga tgtgatccgc ctgctgttga
     3901 agatgatgca caagccggaa gcgttattgc gccgcgtcac cgatcgcccc ggtcacgatc
     3961 gacgctactc gctcgacgtg tcgaagctgc atgcgctggg ctttgagcag agcgcgacct
     4021 ttgagcagga actcgaagca acggtcaaat ggtataccga gaatgaatgg tggtggcgca
     4081 agatcaagac cggcgaatat ctggaatatt ataagcaaca gtatgggcgg cggctggcag
     4141 aaagcgaaaa gatcaattag caattttcac tgaacaatga tcaataaagc cccgtcgagg
     4201 aaaatttctt cgatggggct ttgtgtttcc tcacccccgt cccctctccc gatgacttac
     4261 tgacgaatgt tgcggcatga tcttgatcaa gtcatcggga gaggggtgag cgatctcttg
     4321 atatgaatga tcgaggcggg gtgaggcaaa aaaaagcgcc cgatgaattc aactccatca
     4381 ggcgcgattt actcattact cgttactcat tactcgtcac tttaacatcg gcatcttgat
     4441 gcccgtcttc ttcgcaaact cgatcgctgc tggataacct gcatccgcat gtcgcacgac
     4501 gcccatgccc ggatcggttg ttagcacacg ctccaagcgt ttcgccgctt cgggcgtgcc
     4561 atcggcgacg atgacttgtc ctgcatgttg actatatcca attccaacgc cgccgccgtg
     4621 atggaaactc acccacgtcg cgccgccgac tgcattgatc atcgcattga ggatcggcca
     4681 atcgctgatc gcatccgagc cgtcgagcat cgcttcggtc tcgcgtcgcg gcgaggcgac
     4741 cgaacccgcg tcgagatgat cgcggccaat cacgatcggc gcagagactt cacctgttgc
     4801 gactaactcg ttgaacttca agcccgcctt tgcgcgttca ccgtagccga gccagcaaat
     4861 tcgcgcaggc aagccttgaa acgcgacgcg cttctgcgcc aagcgaatcc agcgttccaa
     4921 cgatttatct tcagggaaca actccaggat cgcttcgtcg gtgcggaaca aatctttttc
     4981 atcgccgctc aacgccaccc agcgaaacgg gcctttgcct tcgcagaaca gcggtcgaat
     5041 gaaggcgggc acaaagcccg gataatcgaa cgcgttctgc acgcccgcat cgaacgcgcg
     5101 ctggcgcaag ttgttgccgt aatcaaacac gatcgatccg cgcttttgaa aatcaagcat
     5161 cgcttgcact tgccgcgcca tcgagtgcat cgatcggcgt gtaaactcag cgggatcagt
     5221 tgatctcagc tttgccgcgt cctcgatcga catgccgtgc ggcatgtaat acaacggatc
     5281 gtgcgcgggc gtttgatcgg ttaccacgtc cggcacaatg ccacgcagga cgagttcagg
     5341 aaaaatatcg gctgcgttcg caatcagccc gattgaaagt ccttgccctt ggtccttggc
     5401 atcttgcacc agcgtcatcg cttcttcaag cgtatcgacg acgacatcga catactttgt
     5461 ttccaatcgg cgttgcgcgc gcgccagatc gacttcgacg acgaggccga cgccgccgtt
     5521 catcgtaatc gccaacggct gcgcgccgcc catgccgccg agacctgcgg tcaatacaaa
     5581 ttttcctttc aatccttcag tccaattttg ttgccgcgca agttcagcca gcgtttcata
     5641 cgtgccttgc aaaattccct gcgagccgat gtagatccat gaacccgctg tcatttggcc
     5701 atagaccatc aacccttcgc gctcgtattg atcgaattgt tcctgcgtgg cccaatgcgg
     5761 cacgatgttc gagttagcga tcaatacacg cggtgcatca cgatgagttt tgaatacagc
     5821 gaccggctta cccgattgaa ccagcagcgt ctcatcgtct tccagttctt tcaaggtggc
     5881 aatgatcgca tcatatgctt cccacgatcg agcggcgcgc ccacgcccgc catacacgat
     5941 caaatcatcc ggcttctcgg ccacatccgg atcgagattg ttcatcagca tgcgcatgac
     6001 tgcttcttgc tgccagccct tgcaggtgat ggtggtgccg cgcggggcgc gaatgattct
     6061 ggacatggtt cacctctaaa aagaaattat cccctcattg cgagcgccga aagggcgaag
     6121 cgatctcccg atagagttga tcagtccaat aagattgctt cgtcgcggag tttatcctga
     6181 gcgaagtcga agggcttctc gcaatgacga tcaatctgca ttgtaacttc atcgcggctt
     6241 tttctcaaat tgaaattaga tcattgatca ttactgacgg ctatgctaaa ctgagcgcat
     6301 gactgattac gcgccgtttg ccgcactgta cgatttattc tatgccgact tcgaagacga
     6361 catcgaaatg tatcgcggtt ttgccgatcg gaccggcggc gcgatcctgg aaattggatc
     6421 aggcacagga cgcgtctcga tcgcgctggc gggtgaagag catcacgtga cgggcgtgga
     6481 actatcggat gaaatgatct cgctggcgca gaagaaaatc gaagcgagcg gactttcaaa
     6541 gcaggtgaac ttgatccagg ccgaaatgcg ccgcttcacg ctcgacgatc atttcggctt
     6601 ggcgattgtg ccgctcaaca ccttcctgca caacctcact ctcgacgatc aactttccac
     6661 tttgaattcg atcaagaaac atttgacgcc caacgggttg ctcgtgctcg attgtttttt
     6721 gcctgacccg acgaatgccg atgaccaccg cttgatcttg cagcgcagcg tgatcgatcg
     6781 agcaacaggg acagcggcgc agttgtttct gtcgcgcacc accgattggg ccaatcagcg
     6841 ccaggacatc acctacttcg tcgatcgctc ggatcgatcg gggcaagtgc agcgcaatgt
     6901 gttcaacact tcgttccgat tcatttatcg gaatgaaatg cagttgttgt tgaaacaggg
     6961 cggcttcgat ctcaaagaag tttacggatc gtacgaactc gatccgtttg atgcgtcaag
     7021 cgacaaaatg attgtcgtgg cgacaccgac ttaaggaggc tgtatgagta aaatgcgcat
     7081 caccgtcgag tactgcgcgc agtgaggcta caccgatgaa gccgtcagtt tgacggacga
     7141 aattttgaaa gagtttgaat ggcagattgc cgagtggatg ctcattccgt cgaagggcgg
     7201 cgtgttcgaa gtaacgatcg atggcgaatt ggttttcagc aagcagcagt tggaccgcca
     7261 cgcggaagag ggcgagattc ggaaattgat cgaagcgcgg ctgccgaaag aataaacggt
     7321 aggggcaagt tttatacgtg ctactacttc tctcttcgtt tcgcttcctc gatccaactt
     7381 ttgatgattg tctccaacac atcaagcggc acggcgccat cgcgcagcag cacatcatgg
     7441 aactcgcgca cgtcgaactt tgcgccgagt tcgcgctcgg cgatcgatcg gagttcacgg
     7501 atttttagtt cgcccgtttt gtaagccacg gcctggcccg gcatcgaaat atatcgatcg
     7561 acttcgttgc tgatgttcaa caaggtgagc gccgtattgt ccgccatcaa atcgatcgcg
     7621 cggtgacgtg gccagccgag ataatgcagg cctgtatcga ccaccaagcg acaggcgcgc
     7681 cacatctcgt aagtgagccg gccaaaatca ttgtaggggt cttgataaaa acccacctca
     7741 agtcccaatc gctccgaata caatccccag ccttcaacga acgcggtgaa accggtgaag
     7801 cgccggaaga ttggaatgtt ttccagttct tgttgaagcg cgatctgcaa atgatgtccc
     7861 ggcacggctt catgcagtga caacgcttcg agttcgtaca acgggcgcgc tttcaagttg
     7921 taggtattca cgtaatagaa accggcgcgt gtgccatcgc ccgcgggctg aaagtaatag
     7981 gcggtcgtcg tgctcggcgc ggcgtaatcc ggcacaggcc gaatgccata cggcatgcgc
     8041 ggcatcgtct tgaaaagttt cggcagttcg ccgtccatct tctttaaaat aaaagacact
     8101 tctttcatca aggcgtccgg cgtcgagaca tagaagcgcg gatcggtgcg caggaattcc
     8161 acgaagtctt tgaaacttcc attgaattca actttcttga taatcgtttc catctcggct
     8221 cgaattcgtt tcacttcgct aagccccgtg tcatgcactt gctgcggtgt gagattcaat
     8281 gacgtgaaca agcgcacgcg atgttcgtag aatgctttgc cattcggcag cgacgaagcg
     8341 gcgatgtccg atcgcgcggc aggcaggtat tcctcggtca cgaattgcat gagcgatcga
     8401 tacgcgggcg ttaccgaagt ctcgatcgct tttctggccc gatcagtaaa atctttttgc
     8461 tgagcggcat ggatgttgtc aggaaattta ttaaagggcg cgaacaataa acttcgggcg
     8521 gcatcctcaa tgagatgcgg ctgtatggaa tccgcgacac tgttcaaggc gacggcgggc
     8581 ggcatgtagc ccgatcgaat gccgacgcgc atcagttcga tgtattgcgt catatattct
     8641 ttgaagccgc gcaagcgcgc aatgtagttt tcgtaatctc gcgccgattc aagcgccaga
     8701 aagttgggca gttcggcgaa gttcgtatga aagccgccca tccgatcgat cggcatgaga
     8761 tacgatcgat attccagttc ggccacatcg tcaagcttga cgcgccgaaa aatatcgagg
     8821 ttgagctgat ccgatcggga cagctgcgcc cgatcgatct cgccggatcg atcgagaaaa
     8881 ttgcgcgctt gatccaagcg gcgttgataa tcggcttccg tcacggccgg caagcgatcg
     8941 tcgtagcggt gatcgccgac atcggtcgcg aacagcgggt cttctttcaa gcgatattcc
     9001 cattcgtcgt tgaggagttg ccgaagttga tcagttgcgg tgttggtcat gatattccct
     9061 ttactcacga cctggaaagg ccgataagat tttgtcctga atgatcgggg tcaagcgggc
     9121 aacgagatcg accgggccgg agagcgagat gccgaggtac gtttcagcgt tgacttcgac
     9181 ggtgccgcga tcgagttgga acttccaata accaatctct tgcgagccgg caccttcggc
     9241 cgttcgctga tcaatcgtct gcgcgccttc cgcggtcagc gcgtcgaaca aggcttgtag
     9301 cattttatga tcgtattcag gacccagatt gaaagtctgc tgcatggctg cctcaaaaga
     9361 tgaaggatcg atcgtgccgc gagtgtttgg gctgatagaa ccagcgatcg tctttccgga
     9421 ttgcgaggcc ttctgcccgc aactgcttca agatcgatgt gagttcttgg gccgaatagg
     9481 tacagtgatc gagataaccg atgagaatct tgcaaatttc atcatcgttg aaagtgcgcg
     9541 ccatgggtta aagtctacct catgacgcgc aaaaccaaaa acgcccgatc gagatcaatc
     9601 gggcgtttga aaaccactca ttacttaccc tagcagcgat tccaacttat cgaccatgcc
     9661 tgataacacg gcaaaggcag catcgaccgc ctgcggcttc gatagatcga cacccgccag
     9721 gcgcagcgca tcgagcgaat acatggagtc gccggctttc aggaagttaa tgtaatcgtt
     9781 ggccgcgcct ttttcgccgg acagaatccg tttcgccagc gcgtgcgcgc cggcaatgcc
     9841 ggtggcatat tgatacac
//
LOCUS       DASRTJ010000432         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_254254,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000432 DASRTJ010000000
VERSION     DASRTJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254254"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2362)
                     /locus_tag="VFF70_08855"
     CDS             complement(<1..2362)
                     /locus_tag="VFF70_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY44843.1"
                     /translation="MLDDVTRKQIAVLHEQPSAIDQLPSALSQMIVPLVSRGQLIGLM
                     YAENHALFGSFAQIDIDLVSAFANQTASAIENARLFQGLEQRVAERTAELNARVDELA
                     IINSVQAGLASKLDLQAIIDLVGDKLRDILNSDDIGIRLYDEQTDRVHYLYEFEHGQR
                     LTVDPMKPSAMFQQQRTDRLPVFGGTAEISRKYKLMMFPGTEQSKALANVPIIAGDKV
                     IGGISVESFEREDFFDESNIRLMQTIASSMGVALENARLFDETQRLLKETDQRAAELA
                     LINSVQEGLASKLDFLAIVDLVGDKVCDIFGSQDMSIGLYDRASNFMTMPYFIESGKR
                     YPIEPTMLTPGFSAHVIRTRQPLIINEDQSRRAQELGARMIGDTDPAASAPIDEQSYL
                     GVPILRGDEAIGILTLYENHKHAFTEAHVNLLQTLASAMSVALENARLFDETNQRAAE
                     LALINSVQEGLASKLDVQSIYDLIGDKIRDIFDAQAVSIATYDLEKNLRTYRYVIEKG
                     QRYYPEPSALTPISHYFIRSRQPLLINRDYVQAMAALGLPSVAIPGTEMTMSSITVPL
                     LVGGEVKGVIALDNVDREDAFSESDLRLLETLASSMSVALENARLFDETNQRAAELAL
                     INSVQEGLASKLDVQAIYDLVGDKIHDLFDAQFVMITAYDVAHNTMSTSYGIEDNKRY
                     YPPPIVLTDISHYLIRTRETLLVNENWAQRMAELGIQPRIAGRTDRWPKSMVFVPLMV
                     GGEVKGSFSLQNMDRENAFSESDVRLLETLASSMSVALENARLFDET"
BASE COUNT          539 a          670 c          644 g          556 t
ORIGIN      
        1 tggtctcgtc gaacaggcgc gcattctcca acgccacgct catcgaggaa gccaatgttt
       61 ccaataagcg cacatccgat tcgctgaagg cattttctcg atccatgttc tgcaaactga
      121 agctgccttt gacctcgcca ccgaccatga gtggtacgaa caccattgat tttggccagc
      181 gatctgttct tccggcgatt ctgggctgaa tgcccaactc agccatgcgc tgggcccaat
      241 tctcattgac gagcagtgtc tctcgcgtcc gtattaaata atgagagata tcggtcaaca
      301 caattggcgg cggatagtaa cgcttgttat cttcgatgcc ataagacgta ctcatcgtat
      361 tgtgcgccac atcataggcg gtgatcatca cgaactgcgc atcgaacaga tcgtgaatct
      421 tgtcgccgac gagatcgtaa atggcctgca catccaactt ggaggcaagg ccttcttgca
      481 cgctgttgat cagcgcgagt tcggcggcgc gctgattggt ctcatcaaac aggcgcgcat
      541 tctccagcgc cacgctcatc gatgaagcga gcgtttccaa taaacgcaga tcggattcgc
      601 tgaacgcgtc ttctcgatcg acgttgtcca gagcgataac gcccttcact tcgcccccga
      661 ccagcagcgg cacggtaata ctggacatgg tcatttccgt gccgggaatg gcaacggagg
      721 gtaaacctag agctgccatc gcttgaacgt aatctcgatt aatcaagagc ggttggcgag
      781 agcggatgaa atagtggctg atcggagtaa gcgccgacgg ctcaggatag taacgctgac
      841 ccttctcgat cacgtagcga taggtgcgca gattcttctc gaggtcatac gtggcgatcg
      901 agacggcttg cgcgtcgaag atgtcgcgaa tcttgtcacc gatcagatcg tagatcgatt
      961 gcacgtccaa cttggaggcg aggccctctt gcacactgtt gatcaaggcg agttcggcgg
     1021 cgcgctgatt cgtctcatcg aacaggcgcg cattctccaa cgccacactc atcgctgaag
     1081 ccagcgtctg caacagattg acgtgcgctt cggtgaacgc gtgcttgtga ttttcataga
     1141 gggtcagaat gccaatcgct tcgtcgcctc tcaagatggg cacgcccaaa taactttgtt
     1201 cgtcgattgg cgcgctggcc gcaggatcgg tgtcgccaat catccgcgcg cccaattcct
     1261 gtgcccggcg agactgatct tcattgatga tcaacggctg gcgcgtacga atgacgtgtg
     1321 ccgagaaacc cggcgttagc atcgtcggct cgatcggata acgcttgccg ctttcgatga
     1381 agtacggcat ggtcatgaaa ttgctggcgc gatcgtacaa cccgatcgac atatcctgcg
     1441 atccgaagat atcgcagacc ttatcgccaa ccagatcgac gatcgctagg aaatcgagtt
     1501 tggaggccaa cccttcttgc acgctgttga tcaaagcgag ttcggcggca cgctgatcgg
     1561 tttctttcag cagccgctgg gtctcatcga acaggcgcgc attctccagc gccacgccca
     1621 tcgacgacgc gatcgtctgc atcaaacgga tgttggactc gtcgaagaaa tcttcgcgct
     1681 cgaaactctc caccgaaatt ccgccgatga ctttgtcgcc cgcaataatc ggcacgttcg
     1741 ccagcgcttt ggattgttcc gtgccaggaa acatcattaa cttatatttt cgtgaaatct
     1801 cagccgtccc gccaaagacc ggcagccgat cggtgcgttg ttgttggaac atcgctgagg
     1861 gtttcatggg gtcaacagtt agccgctgac cgtgctcaaa ttcatataga taatgaaccc
     1921 gatcagtttg ctcgtcatac aagcgaatac cgatgtcgtc cgaattcaaa atgtcgcgta
     1981 gtttgtcacc aaccaaatcg atgatcgcct gcaagtccaa cttggaagca agtccggctt
     2041 gcacgctgtt gatgatcgcc agttcgtcca cgcgcgcgtt gagttcggcg gtgcgctcgg
     2101 ccacgcgctg ctccaggcct tgaaacaagc gcgcattctc gatcgcggag gcggtctgat
     2161 tggcgaaggc cgaaaccaga tcgatatcga tctgcgcgaa cgaaccgaac aatgcgtgat
     2221 tttcggcgta cattaatccg atcagttgtc cgcgcgaaac gagcggcacg atcatctggc
     2281 tgagggcgga tggcagttgg tcgatagcag atggttgttc atgcaagacg gcgatttgct
     2341 tgcgcgttac atcgtctaac aaatcggcaa ctccttgaag cgcggccagt tcttcagcct
     2401 ctgtgtagc
//
LOCUS       DASRTJ010000433         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_329906,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000433 DASRTJ010000000
VERSION     DASRTJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329906"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VFF70_08860"
     CDS             complement(<1..1019)
                     /locus_tag="VFF70_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HZY44844.1"
                     /translation="MEFHVSRYARDKYQFDRALFALDGNVIFANFLAARTFAQKINQQ
                     RDLVNFPEQAVKAGQINALGLIDELLHFVVQQYREQKNPQVMKAALTWLPAKVSAEAL
                     DAALRKFADEFPPIKIYRRELSIDEYFADPANRENTLEEMIMLWLSNMNPACAPFMEL
                     FDDATLEKEMAYAPMLASLRDFFETQPKFGPDNQNLIDMLRAPALANPHSLTAQLEFI
                     LQRWGVVLTKYLYRLLGGLDLIKEEEKIIFIGTQPMRVPMFGEAKLRGDRLAEAEPEN
                     FTLDREWMPRLVLIAKNAYVWLDQLSKNYQRAIYRLDQIPDEELDQLARWGFTGLWLI
                     GLWQR"
     gene            complement(1109..2095)
                     /locus_tag="VFF70_08865"
     CDS             complement(1109..2095)
                     /locus_tag="VFF70_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZY44845.1"
                     /translation="MEYRRLGKAGIKVSTLSFGAWVTFGQQYGEDVAYDIMKAAYDAG
                     VNFFDNAEAYADGRAEEVMGAALKRAGWKRSDLVISTKIFHGGRGPNDRGLNYKHVIE
                     GTNRSLRRLQLEYVDLIFCHRPDRETPIEETVRAFTQVIHDGKAFYWGTSEWEADEIA
                     QAILIARQEHLIPPTMEQPEYNMFYRDRIERDYLRLFSDFGYGTTIWSPLASGLLSGK
                     YNQGMPIDTRASLPGYEWLKADFEKEEAKRNIEKVKLLMPIASDLGCTMAQMAIAWCA
                     KNPNVSTVITGASRSEQVVENMKAIDVVAKLTPDVMESIEKILDNKPKAPDR"
     gene            complement(2241..>2648)
                     /locus_tag="VFF70_08870"
     CDS             complement(2241..>2648)
                     /locus_tag="VFF70_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZY44846.1"
                     /translation="QQSWLRLIGGTYLLYLGIRTFRSRPAELSTTDGRRGLVRAYTST
                     FFLMLTNPLTIFSFIAIFAGIGTGSTNSGGLSPLLIVVGVFLGSCAWWLFLVAITGIF
                     RSRLNGRSMQWINRSSGLIISAFAIVILIGLIK"
BASE COUNT          636 a          760 c          598 g          654 t
ORIGIN      
        1 ctgcgctgcc acaatccgat cagccacaga ccggtaaagc cccaccgcgc gagttgatcc
       61 agttcttcat caggtatttg atctagccga taaatcgcac gttgataatt tttggaaagt
      121 tgatcgagcc atacataggc attcttggcg atcaacacca ggcgaggcat ccactcgcga
      181 tcgagcgtga agttttccgg ttcagcctcc gccaaacgat cgccgcgcaa tttcgcttca
      241 ccgaacatcg gcacgcgcat cggttgggtg ccaatgaaga tgatcttctc ttcttctttg
      301 atcagatcga gtccacccaa caatcgatac aaatatttag tgagcaccac accccagcgc
      361 tgcaagatga attccaattg cgctgttaac gaatgtggat ttgccaacgc gggcgcgcgc
      421 agcatatcga tcagattttg attgtcgggg ccgaacttcg gctgagtctc aaagaagtcg
      481 cgcaggctcg ccagcatcgg cgcataggcc atctctttct caagcgttgc atcatcgaag
      541 agttccataa agggcgcgca ggcgggattc atattggaca accacaacat gatcatttct
      601 tccagcgtgt tctcgcgatt tgccggatcg gcaaagtatt catcgatcga caactcgcgc
      661 cgatagattt ttatcggtgg gaactcgtcg gcaaattttc gcagggcggc gtccaacgcc
      721 tcggcgctga ctttcgcagg cagccacgtt agtgccgctt tcatcacttg cggatttttc
      781 tgctcgcgat actgctgcac cacaaaatgc agcagctcat cgattagccc caacgcattg
      841 atctgtcccg ctttaacggc ctgttccgga aagttcacca gatcgcgctg ctgattgatc
      901 ttctgcgcaa acgtccgagc cgcgaggaaa ttggcgaaga ttacattgcc atccaaagca
      961 aacagcgctc gatcaaattg atacttgtca cgtgcgtatc gcgaaacgtg aaattccatt
     1021 ggaaaattct ttctaaccac aaaaacgcta aagcgccaag aaaatctttg ttatcttcgc
     1081 gtctttgcgc cttcgcagtt tggatttatt atcgatcggg cgctttgggt ttgttatcca
     1141 aaatcttttc aatgctttcc atcacatccg gcgtcaactt cgccacgaca tcgatcgcct
     1201 tcatattttc aaccacttgt tctgatcgcg atgcgcccgt gatgactgtg ctgacattcg
     1261 gattcttcgc gcaccacgca atcgccatct gcgccatcgt gcaacccaag tcgctcgcaa
     1321 tcggcatcaa gagttttact ttctcgatat tgcgtttggc ttcttccttt tcgaagtctg
     1381 ccttcagcca ttcataaccg ggcagtgatg cccgtgtatc gatcggcatg ccttgattat
     1441 attttcccga caacaagccg ctcgccagcg gactccagat cgttgtgccg taaccgaaat
     1501 cgctgaacag gcgcagataa tcgcgttcga tccgatctcg ataaaacata ttgtattccg
     1561 gctgctccat cgtcggcgga atcaaatgtt cctgccgtgc gatcaggatc gcttgcgcga
     1621 tctcgtcggc ttcccattcg ctagtgcccc aataaaatgc tttgccgtca tgaatcacct
     1681 gcgtgaaagc gcgtacggtt tcctcgattg gcgtctcgcg atcgggtcga tggcagaaaa
     1741 tcaggtcgac gtactccaat tgcaagcgcc tcaacgatcg attcgtgccc tcaatgacgt
     1801 gcttataatt caagccacga tcattcgggc cgcgtccgcc atgaaaaatt tttgtggaga
     1861 tcactaagtc cgatcgcttc cagcccgctc gcttgagtgc ggcgcccatc acttcctcgg
     1921 cgcggccatc cgcgtacgcc tcagcattgt cgaaaaaatt cacgcctgca tcgtaggccg
     1981 ctttcatgat gtcatacgcg acatcttcac cgtactgctg gccgaacgta acccatgcac
     2041 caaacgacag cgttgaaact ttaatgcctg ctttacctaa acgtcgatat tccattgctg
     2101 ctccttgctt tcgtcattgc gagccgccgt aggcggcgtg gcaatctcag tctcgatcgg
     2161 gagattgctt cgtcggccta atgtcctcct cgcaatgaca caccaacaat atttattttc
     2221 gattgattat agcacgccaa tcatttgatc aagccgatca agatcacgat cgcaaacgcc
     2281 gagatgatta accccgagct ccgattgatc cactgcatcg atcgaccatt tagcctcgat
     2341 cgaaagatgc cggtaatggc gactaagaat aaccaccacg cgcacgaacc cagaaacacg
     2401 cccaccacaa tcaacagcgg tgataagccg ccgctattcg tactgcctgt accaatgcct
     2461 gcaaagatcg caatgaaaga aaaaatcgtc agtggattag tcaacatcaa aaaaaacgtc
     2521 gatgtgtagg ccctgacaag cccgcgccgc ccatcggtgg ttgatagctc tgccggacgc
     2581 gatcgaaatg tcctgatgcc aagatacaac aaatatgttc caccgatcaa acgcagccac
     2641 gattgttg
//
LOCUS       DASRTJ010000434         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_26979,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000434 DASRTJ010000000
VERSION     DASRTJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26979"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFF70_08875"
     CDS             <1..543
                     /locus_tag="VFF70_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44847.1"
                     /translation="DDLMSRFLNHAQPITVTKNFKIYPLDVNALRTSIERWVSTVETL
                     DHTATSVPISFEGKIDLIGYDRAMTVSVGSTLAIFTAWHVTSEGQPLSTAIFIHILNP
                     DGSIAAQDDRLGYPRHNWHTGDEFVQVHHLSIGNLPPGKYSIEMGIYSRDTNVRWLAQ
                     DRSGQSIGDRILIGSIEIVP"
     gene            540..2285
                     /locus_tag="VFF70_08880"
     CDS             540..2285
                     /locus_tag="VFF70_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44848.1"
                     /translation="MIAQRLKTIAPAILIVLAYAIVTVIALNNLIAHINTAMPGTVNV
                     QASDQIQPSEFDIFYWNVWWVRHAVFDLHVSPLYTNFIAYPFTSPLAGHTLALLWGLI
                     SAPFQHVLGLITTYNLIIVVCFVAAAMSMYAFVRRHVTQRGVAALAGLMFAFTPAMLQ
                     RASVGHLDKLSIVWLPLILLVWDKVIESRRRTWAIVLGVAVWFSGLTDFQQTMWALLL
                     LVPYAAYTLISPQRRGGAEQNKNLNVSASTRLVVIALAAFIIPSLFAPLPQLLEANRL
                     NYPPARLEDTAAFAFPIQNFFNPSDNGDFSIGLLLPIGTLLSVIFLRRNGKPWLWFLI
                     GLGCFVLALGPYIDIGATRVPLPYSFVHMLLGNQYRTPMRFATPGVFAWTMLIALTLD
                     HFFVWLASRLTPHTSRLTHYSLLITLSALFIFDYHLLQPFPIAYMPDYQIYHTISVTP
                     GDFTVLELPIGVRTGFAAVGRGEYLQYYEPIHQHPIPSGYLSRLPNEITDYFYFDPLI
                     GALTLSHGLPPQLQVDAELTQLISDWHIGYVILHRDMLEPGRVKSFGDLLNRQSALVK
                     IGEEGPLVIYRAKTP"
     gene            2354..4423
                     /locus_tag="VFF70_08885"
     CDS             2354..4423
                     /locus_tag="VFF70_08885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY44849.1"
                     /translation="MKSKLEWIAVIGVMLIAAAFRLIALNDVPPGLHHDEVIIGQEVK
                     EILNGHLSIYFTGGYGQEPLYHYLTAGMFAAIGANAFVLRLASAFFALIGLAITYRFT
                     RKLFSPIVAIGTLAWMSISLWPIFFSRVGLRGITLPVFTMLTAYFLWRAVHGTPLRFA
                     TRAVARTTQYVIAGGLLGLSIYTYQASRVFPLIFLIFFISLFISRSSLITHHLVRSFI
                     IFFIAAAIIAAPLIIYLTAINPSAEQRIGDLSGALNKLAAGDPSEVLQSTLNTLGMFT
                     IRGDAVPIYNVSNRPVFPEPIGAAMFYLGLLACVWRWKQPHYALMLIWFFISLVPAMV
                     TPFSPNFVRTIASWPTPFVLVGIGMNEVIQLIGKRIQKPEFRSLNWAAGFFVVALALN
                     VVMTAKDYFINWPTGDYVRFWQQATWTQAVRMLNTDASNETVAASGLSINDFDPQTFD
                     LLGLRSDIKVKWFDCRNAMLFPHMTYPTRYLTPDFFPCDADLWQRYLFGASLIAQPRW
                     PDSGNVIFSLYEFNPYQLSIGLREAGNTSNWSVGGEGFDAKNPEADLIQVNGINFSGL
                     NFFGYFFDRKAVKPGETVEIQTVWKMTAPLSPPLSIFIHITAPDGSIVAQWDGLDVGV
                     ASLEPYDLFVQRHRVQIPANAPIGPYRISLGVYHPDSGVRLKATVGQQSIGPIDSIVL
                     GTLTLVK"
     gene            4458..>5097
                     /locus_tag="VFF70_08890"
     CDS             4458..>5097
                     /locus_tag="VFF70_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44850.1"
                     /translation="MPDRVDLSLVIACYNEEFELEDSLRQVIEILDSTRWTYEIIFVD
                     DCSHDRTREIIDRLMSVYQAKNFRKIFHEHNTGRGRAVTDGFRIAIGDVIGYIDIDLE
                     VHARYIPTMMLAIEQGADVATAHRIYKVQPRLFHRFVLSQGYVWLMRHMLDAPLKDTE
                     TGYKFFKREKIWPILDRAKDEHWFWDTEIMVRSLLAGLKIVELPCLFVKRYDK"
BASE COUNT         1097 a         1279 c         1315 g         1406 t
ORIGIN      
        1 gacgatctca tgtcgcgttt tctcaatcat gcccagccga tcacggtgac gaaaaacttc
       61 aaaatttatc ctttggatgt taacgctctg cggacatcga tcgaacgttg ggtcagtaca
      121 gttgaaacgc ttgaccacac tgcaacttct gtgccgatct catttgaggg caagatcgat
      181 ctgattggat atgatcgtgc aatgactgtc tcagtcggat cgacgctagc tatctttaca
      241 gcgtggcatg tgactagcga agggcagccg ttatcaaccg ccatcttcat tcacatattg
      301 aatcccgatg gctcgatcgc ggcacaggac gatcgattag gctatccgcg tcataactgg
      361 cataccggcg atgaatttgt gcaggtgcat catctctcga taggcaatct gccgcccggc
      421 aaatattcga tcgagatggg aatctattcg cgtgacacca acgtccgctg gcttgcgcag
      481 gatcgatcgg gtcaatcgat cggcgatcgc atcctgatcg gatcaattga gatcgtgcca
      541 tgatcgctca gcgcctcaaa acgatcgcgc cagccattct catcgttctc gcgtatgcga
      601 tcgtcacggt gatcgcattg aacaatctga tcgcgcacat caacaccgcg atgcccggca
      661 ccgtcaacgt tcaagcctcc gatcaaattc aaccctccga gtttgatatc ttttattgga
      721 atgtgtggtg ggtgcggcac gcggtgtttg atctgcatgt cagcccgttg tacaccaact
      781 tcattgccta tccattcact tcgccgctgg cgggacatac gctggcgttg ctgtggggtt
      841 tgatcagcgc gccgtttcaa catgtgttgg gcttgattac aacttacaac ttgatcatcg
      901 tggtctgttt tgttgcggcg gcgatgtcga tgtatgcgtt tgtgcggcgg catgtgaccc
      961 agcgcggcgt ggccgcattg gccggattga tgtttgcttt cacgcccgcc atgcttcagc
     1021 gcgcatcggt cgggcatctc gataaacttt cgatcgtgtg gctgccgctg attctattgg
     1081 tgtgggacaa agtgattgaa tcgcgtcgcc ggacgtgggc aattgtttta ggtgtcgctg
     1141 tgtggttctc agggctgacc gattttcaac aaacgatgtg ggcgttgctg ctgctcgtgc
     1201 cttatgctgc ttacactttg atttcaccac agagacgcgg aggcgcagaa caaaataaaa
     1261 atctcaacgt ctctgcgtcg acacggttag tcgtaatcgc cttagcagca ttcatcattc
     1321 catcactctt tgcaccgtta ccacaattgc tcgaggcgaa tcgattgaat tatccgccgg
     1381 cgcgattgga agataccgcg gccttcgctt ttccgattca aaactttttc aatccaagcg
     1441 ataacggcga cttctcgatc ggtttgctgc tgccgattgg aactttgctc tcggtgatct
     1501 tccttcggcg caatggcaag ccttggctgt ggttcttgat cggactcggc tgcttcgttc
     1561 tcgcgttggg gccgtacatc gatattggag cgacgcgtgt tccattacct tactcgtttg
     1621 ttcatatgtt attgggcaat caatatcgca ccccgatgcg cttcgcaacg cccggcgttt
     1681 ttgcgtggac gatgttgatc gcattgactc tagatcattt ctttgtctgg cttgcctcac
     1741 gcctcacgcc tcacacctct cgtcttactc attactcgtt actcattact ttgtctgctc
     1801 tctttatctt cgactatcat cttcttcagc cattcccgat cgcttatatg cccgactatc
     1861 agatctatca cacgattagc gtgacccctg gtgatttcac agtattagag ttgccgatag
     1921 gcgtgcggac aggttttgca gctgttggcc gcggtgaata cttacaatac tatgagccaa
     1981 tccatcaaca tcccattcca tccggttact tgtctcgact gccgaatgag ataaccgact
     2041 atttctattt cgatccattg atcggcgcac tgacgttgtc acatggcttg ccaccgcagc
     2101 tgcaagtcga tgcagagttg acgcagttga tcagcgattg gcatatcggt tatgtgatcc
     2161 tgcatcgaga tatgctggaa ccggggcgcg tgaagtcatt tggcgatctg ctgaatcggc
     2221 agtcggcgtt agtgaagatc ggcgaagaag ggccactggt tatttatcga gcgaaaacac
     2281 catgagcgat caagaaagtt ttttaccgct gagtacgcaa agtccgcaaa gaaatacatc
     2341 tgataactct gcagtgaaat ctaaactgga atggatcgcg gtgatcggcg tgatgttgat
     2401 cgcggccgcg tttcgtttga tcgcactgaa tgatgtaccg ccgggtttgc atcacgatga
     2461 agtgatcatc ggacaagagg tgaaggagat tttaaacggc cacttatcga tttacttcac
     2521 aggcggatat ggccaggagc cactatatca ctacctcaca gctggaatgt tcgcggcgat
     2581 cggggcgaat gcgtttgtgc tgcgattggc ctcagccttt tttgcgctca ttggtctagc
     2641 gattacttat cgattcacgc gcaaactttt ttcgccgatc gttgccatcg ggacgctggc
     2701 ctggatgtcg atctcgctgt ggccgatctt tttctcgcgc gtcggtttgc gcgggatcac
     2761 tttgccagtg tttacaatgt tgacggcata ttttttatgg cgggcagtcc atggaacccc
     2821 gctccgcttc gctacgaggg ctgtcgcccg caccacgcag tatgttattg ctggcggatt
     2881 gcttggcctg agtatttata cttatcaggc gagccgcgtc tttccgttaa tctttctcat
     2941 tttcttcata tccctattca tttcccgctc atcactaatt actcatcact tggtacgctc
     3001 tttcatcatc ttttttatag cagccgccat cattgctgca ccgctcatca tttatctcac
     3061 tgcgatcaat ccctccgcgg aacagcgcat cggcgatctc agcggcgcgt tgaataaact
     3121 tgcggcgggt gatccctccg aagtgcttca atcgacgctg aatacacttg gcatgtttac
     3181 cattcgcggc gatgcggtac cgatctacaa cgtcagcaat cgaccagtgt tcccggagcc
     3241 aatcggcgcg gcgatgttct acctcggctt gttggcgtgt gtctggcgct ggaagcaacc
     3301 gcactatgcc ttgatgctga tctggttctt catcagcctg gttccggcga tggtcacgcc
     3361 gttctcgccg aacttcgtcc gcacgatcgc gagttggccg actccctttg tattggtggg
     3421 gattgggatg aatgaagtca tccagttaat tggcaaaaga attcagaagc cagaattcag
     3481 aagtctgaat tgggcagccg gtttttttgt cgttgcgttg gcgctcaatg tagtgatgac
     3541 tgccaaagat tacttcatca actggccgac gggtgactat gtgcgatttt ggcagcaagc
     3601 aacatggaca caggccgtgc gaatgttgaa taccgatgca tcgaacgaga cggtggcggc
     3661 ttcggggttg agtatcaacg acttcgatcc gcagacattc gatttgctgg gcttgcgatc
     3721 ggatatcaag gtgaaatggt tcgattgcag aaacgcaatg ttgtttccgc atatgactta
     3781 cccaacgcgc tacctcaccc ctgatttctt tccgtgtgat gccgatcttt ggcagcgtta
     3841 tttgtttggt gcaagcttga ttgctcaacc acgttggccc gattcaggta acgtgatttt
     3901 ttcgttatat gaatttaatc catatcaatt gtctataggg ctccgcgaag caggcaatac
     3961 ttctaattgg tcagttggcg gcgaggggtt cgatgccaaa aatcctgaag ccgatttaat
     4021 tcaagttaac ggtattaatt ttagcggatt gaattttttc gggtacttct ttgatcgaaa
     4081 agcagttaag ccgggtgaga ccgtagagat ccaaactgtt tggaaaatga cagcaccgct
     4141 ttcgccgccg ctctcaatct ttattcatat tactgcgcca gatggatcga tcgttgcgca
     4201 atgggatggt ttagatgtgg gtgttgcttc actggaacca tacgatcttt ttgtacagcg
     4261 gcatcgagtc cagattccag cgaacgcgcc gatcgggcca tatcgtattt cactgggcgt
     4321 ctatcatccc gattccggcg tgcgattgaa ggcgacagtc ggtcagcagt cgattggtcc
     4381 aatcgactcg atcgtgttag gcacgttgac tctggtaaaa tgaagcgcac ttaaatgcaa
     4441 gcactggatg gactgcaatg ccggatcgcg ttgatttgag tttggtgatc gcctgctata
     4501 acgaagaatt tgaattggag gacagcctgc gtcaggtgat cgaaatactg gacagcacgc
     4561 gctggaccta cgagattatt ttcgtcgacg attgcagcca cgatcggacc cgcgagatca
     4621 tcgatcgtct gatgagcgtt tatcaggcta agaacttccg gaaaattttc cacgagcaca
     4681 atactggccg cgggcgtgcg gtgaccgatg ggttcagaat cgcgatcggg gatgtcatcg
     4741 gctacatcga tatcgatctg gaagtacatg cgcgctacat cccaacgatg atgctggcga
     4801 tcgagcaagg cgcggatgtg gcgacggcgc atcgcattta caaagtgcag ccgcgcttgt
     4861 tccatcgttt tgtgttgagt caaggctacg tatggctgat gcgtcacatg ttggacgcgc
     4921 cattaaagga taccgaaacc ggctacaaat ttttcaagcg cgaaaaaatc tggccgatcc
     4981 tcgatcgagc aaaagacgaa cactggtttt gggatacgga gatcatggtg cgctcgctgc
     5041 tggctggatt gaagattgtc gaactgccgt gtttgttcgt caaacgctac gacaaaa
//
LOCUS       DASRTJ010000435         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_330012,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000435 DASRTJ010000000
VERSION     DASRTJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330012"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(500..850)
                     /locus_tag="VFF70_08895"
     CDS             complement(500..850)
                     /locus_tag="VFF70_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44851.1"
                     /translation="MYPESEILFPSRVIPLLEDLRSEEWQALVEKVKNLPETHVDTLA
                     FSLMMINIGGCLTCDLDSYRASLGCATCAKRTISSFKGSDKILKKQFDEAKKEVGAYL
                     KSNVASAAKQAKAK"
     gene            1112..>2091
                     /locus_tag="VFF70_08900"
     CDS             1112..>2091
                     /locus_tag="VFF70_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44852.1"
                     /translation="MRHRLRNSLIALTLILVWSLPPMIYAQEPPRGIDAVIIFDTSGP
                     LLNQIDQLCPTLLKNLTALQTRGFDVQATPLGVTGPYNCAKSSVKTSIANSTVASDDD
                     WGAAIADVAAHFIWRSNTLRVIIPISNRGPALGDPVDDPGIDRESIGRAIKLAQANHV
                     IVSPLLGAPDRATQPTDRSKLLVLANDLAKGTGGQLIELTDSISDPTHSIFELITRAV
                     QSASNGPLLSIPAAVRTFTCQRDSIKCISFDLGVLLTNAGLAVWFTLILGFSTALLNK
                     SLGHIHQPKMDSMPTDSQAPRLQRIGHRLSTAIDTGANTVRHSFRSLFAP"
BASE COUNT          488 a          598 c          497 g          508 t
ORIGIN      
        1 tatcaggcat ggcgcgcgta aagttagcca gataatcgat cgagccccgc acttgcgaca
       61 gcaatttctc ttcacctgtt acgccgaaca tcgccgatcg gatcgtatcg cgggcaaaga
      121 aagcgatcac gcccagaagg atggtagtga caatgaagat aattcgtttg ggcataaatc
      181 gcctcaaata aatattgcgt aattcgtcct gatcttatgt gcaacttttt ctcgcctgga
      241 aaagattcaa ccgtcattgc gaggcgcgtc tagttgcgcc gatgcaatct cagctgattg
      301 tcgtggagat tgcttcgctt cgctcgtaat gacgttacga ccttgttcgg atcggaaagt
      361 tgcatattgc gttgctgcgt cattccatat cacgaagtac gcataactca tcactcgtta
      421 cttttcacgc agcacgtatc aggttcatta taaaacaaaa agcccggagg catactccgg
      481 gccgatcgag gctaaccgtt tacttcgctt tcgcttgttt ggcggcgctg gcgacgttcg
      541 atttcaaata tgcgcccacc tctttcttcg cttcatcaaa ctgtttcttc agaatcttat
      601 cggaaccttt gaaactgctg atggtccgct tggcgcaggt cgcgcagccc agcgacgccc
      661 gatacgaatc caagtcacag gtcaggcagc caccgatgtt aatcatcatc aatgaaaaag
      721 ccagtgtatc aacatgtgtc tccggcagat tcttgacctt ctcaaccagt gcctgccact
      781 cttcactgcg caggtcttcc aacagcggaa tcacgcggct gggaaaaagg atttcgcttt
      841 ctggatacat tgagcctcac accctgagga tttactacag cggaaatcat aacatatccc
      901 actcagtgaa tcaagggtaa aacccgaaat tcaagggttt tttgccgatt ctcgttcaaa
      961 tccgggcaaa tttagaacat ccgatctata gcccaacgga ttgcgtatat catcgatcat
     1021 tgaccgctag acctcttcag ctttgacaga tgcgcgttgc gatatacaaa cccatgctac
     1081 aatacttcca cctcaatttg atcggagacc catgcgtcat cgattacgca acagccttat
     1141 tgccctcacg ctgattttgg tttggtcact gcccccgatg atttatgctc aagagccacc
     1201 acgcggcatc gacgcggtga tcatcttcga cacgtccggc ccgctgttaa accagatcga
     1261 tcaattgtgc ccgacgctgt tgaaaaattt aaccgccttg caaacacgcg gctttgatgt
     1321 gcaagccacg ccgctgggcg tgaccggtcc ttacaactgc gccaagtcga gcgtaaaaac
     1381 ttcgatcgcg aattcgacgg tggctagcga tgatgattgg ggcgcggcca ttgctgatgt
     1441 cgcggcgcat ttcatatggc gatcgaatac gctgcgcgtg atcatcccta tctccaatcg
     1501 aggtccggcg ctcggcgatc cggtggacga tccggggatc gatcgggaat cgatcgggcg
     1561 cgcgatcaaa ctcgcgcagg ccaatcatgt gatcgtatcc ccgttgctcg gtgcgcccga
     1621 tcgggctacg cagcccacgg atcgatctaa actcttagtg ttagccaacg atctcgccaa
     1681 aggcaccggt ggacaattga tcgaactaac ggactcgatc tctgatccaa ctcactccat
     1741 atttgaattg atcactcgcg cggtccagtc tgcctcaaac ggcccgttgc tatcgatccc
     1801 ggccgctgtg cgaaccttca cctgccagcg tgacagcatt aaatgcatct cgttcgatct
     1861 gggagtgttg ttgaccaacg ccggactcgc cgtgtggttc acactcattc tgggcttctc
     1921 caccgcactg ctgaataaaa gtctcgggca cattcatcag cctaaaatgg atagcatgcc
     1981 cacagactcg caggcgcctc gcttgcagcg gatcggccac cggctgtcaa ctgccatcga
     2041 caccggcgcc aacacagtgc ggcacagttt ccgatcgttg ttcgcgcccg a
//
LOCUS       DASRTJ010000436         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_65164,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000436 DASRTJ010000000
VERSION     DASRTJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65164"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFF70_08905"
     CDS             complement(<1..489)
                     /locus_tag="VFF70_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44853.1"
                     /translation="MRRLFTSILPTVVTALAGLVVFLSFLLPALADWRAALVSIAVII
                     AAFAVVLGFAHLILGVHMQRIRQGKGAFYSLTLILSAMLTLGVLIAERVLPAADPTHP
                     VSMFIFNNIITPMQSSLGALLAIFLAVAAFRMLRRRRTAGAVWFLLSALIVLITQVPL
                     PLA"
     gene            complement(515..1273)
                     /locus_tag="VFF70_08910"
     CDS             complement(515..1273)
                     /locus_tag="VFF70_08910"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HZY44854.1"
                     /translation="MSAPIVIGLIALILFFIFQILYTVRFTAGRRPRFRSIPVFNALH
                     GQMGRAIESGKALHVSVGTGGISGEDMATTLAGLSIVEYLADEAAASGIAPVITTSDS
                     TTLLLAQDTLRRPHVRQGDLSGYQSLSAQQLALNSTQYAAAAMDYLAHDQVSANVMSG
                     AFGVEVALIEHEADKQSLSQMTGAADPRALAIMLASHENVLMGEEIYAAKAYLRARPQ
                     HLASLRTEDVLRWLIVLSISVWFVLNLVQQLAVR"
     gene            complement(1270..1533)
                     /locus_tag="VFF70_08915"
     CDS             complement(1270..1533)
                     /locus_tag="VFF70_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44855.1"
                     /translation="MTTEPVTNKSIWIDRLQQWGLGGVASTLIDVLRPLGFFGSQAIS
                     MASPVLTTFVDPDRLDRLAGLLEDPDRLTQLSRLLAESPEDPS"
     gene            1681..1881
                     /locus_tag="VFF70_08920"
     CDS             1681..1881
                     /locus_tag="VFF70_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44856.1"
                     /translation="MYGRLTGRKRVMIMLLGAIGGAIVAIVFALFARRSIVAIDLIVG
                     AIIGGLLGLSASLGETRRKRSP"
     gene            2015..2431
                     /locus_tag="VFF70_08925"
     CDS             2015..2431
                     /locus_tag="VFF70_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44857.1"
                     /translation="MKTYQYLTIVLAITLLIAACSPAATPTAQPQPTTVPTASGPNTG
                     LQTRPEANAWANAPQAALAARGDLMLKLSVDPDTIVLVSADQIDWPDACLGIQLPGKM
                     CAQVLTSGYKIVLGANGQEYEYHTNKTGTNIQLVTK"
BASE COUNT          696 a          749 c          750 g          541 t
ORIGIN      
        1 ggccaacggc agcggcactt gcgtgatcaa cacaatcaag gccgataaca aaaaccagac
       61 tgcaccggcg gtgcgtcgac ggcggagcat ccgaaaggcc gccaccgcca ggaaaatagc
      121 aagcagcgcg ccgagggatg attgcatcgg cgtgataatg ttattgaaga taaacatgct
      181 gactggatga gttggatcgg ccgctggcag aacccgttcg gcgatcaaca cacccagcgt
      241 caacatcgct gacaagatca aggtgaggct gtaaaacgca cctttaccct gtcgaatgcg
      301 ttgcatgtgg acgcccaaga tcaaatgcgc aaaaccgagc accacggcaa aggccgcgat
      361 gatgaccgca atactcacca gcgccgcgcg ccaatcagca agcgcaggca gcagaaaact
      421 gagaaagacg accagtccgg ccagcgccgt tacaaccgtt gggagaatgc tggtaaataa
      481 acgtctcacg ttctgactcc ggtgcagcga tcgatcagcg gacggctaac tgctgcacta
      541 aatttaacac aaaccagacg ctgatgctta acacgatcaa ccagcgcagc acatcttccg
      601 tgcgcagact tgccaagtgt tgaggccgcg ctcgcaagta agctttggcc gcataaatct
      661 cttcacccat caacacgttc tcgtgcgacg cgagcataat cgctagcgcg cgcggatcag
      721 ctgcgccggt catttgcgac aacgactgct tgtcggcttc atgttcgatc aatgcaactt
      781 cgacgccgaa cgcgcccgac atgacattcg ctgaaacttg atcgtgcgcc agataatcca
      841 tcgccgcagc tgcatattga gtggagttca gcgccaattg ctgggccgat aaagattgat
      901 aaccgctcag atcaccctgt cgaacatgcg ggcggcgcag cgtgtcttga gcgagcagca
      961 aggtagtgga atcggaagtg gtgatgacgg gcgcgatgcc gctggccgca gcttcatccg
     1021 ccagatactc gacgatcgaa aggccggcaa gcgtcgtcgc catatcttca ccgctgatgc
     1081 cgccggtgcc gactgagacg tgcagcgctt tgcccgattc gatcgcgcgg cccatttgtc
     1141 cgtgcagcgc gttgaagacc gggatcgatc gaaagcgcgg ccggcggcca gccgtgaatc
     1201 tcacggtgta aagtatttga aagatgaaaa agaggatcag tgcgatcagc ccgatgacga
     1261 taggtgcgct catgatgggt cctcaggcga ttcagcgagc agccgactga gctgcgtcag
     1321 ccgatcgggg tcttccaata aacccgctag gcgatccaac cgatcgggat caacaaaagt
     1381 tgtcaacacg ggcgaagcca tgctgatcgc ttggctgccg aaaaatccga gcgggcgcag
     1441 aacatcgatc agcgttgagg ccacgccacc caagccccac tgctgcaagc gatcgatcca
     1501 gatagattta ttggtgacag gttcagttgt catgaatgac cgattaacgc ttcaagatcg
     1561 atttatcttg atcgagtagg gttagtatag cacgccatgc ttgaaaagtg aagcgaaaat
     1621 aatattaaaa gttggaaatt ggttgatgga ggttagtggt atactcggcg catgagcgaa
     1681 gtgtatggcc gcctaactgg ccgcaaacgc gtgatgatca tgctgctggg cgcgatcggc
     1741 ggcgcaattg tggcgatcgt attcgcactc ttcgcgcgcc gatcgatcgt cgcgatcgat
     1801 ttgattgtgg gcgcaattat cggcggctta ttgggcttgt cggcgagtct gggcgagacg
     1861 cgccgcaaac gatcgccata atgacgaatg aatttcaatc acggcggctc gatcgatcga
     1921 gccgccgttt tgattcatgg aactttttta aaatcgtatc gtcttaaggg gtgaaagaga
     1981 gccaagggca tccttatcca tcaggagact taacatgaag acttatcaat atctaacaat
     2041 tgttttggcg atcactttat tgatcgccgc ctgcagtcca gcggcaacac cgacggcaca
     2101 gcctcagcct actactgttc cgacagcctc cggtccaaac accggattgc agacgcggcc
     2161 tgaagcgaat gcgtgggcaa acgcgccgca agccgcactg gccgcgcgcg gtgatttgat
     2221 gctgaagctc agtgtcgatc ccgacacgat cgttttagtc agcgccgatc agattgattg
     2281 gcccgatgcc tgcttgggca ttcaattgcc cggcaaaatg tgcgcgcaag tgctcacctc
     2341 gggctacaag attgtactgg gcgcgaatgg acaagagtat gagtatcata ctaacaaaac
     2401 gggcacgaac attcaactcg tgacgaagta agccatgtaa gacaagtggc caacccttcg
     2461 ccttcactca gggcaggctt gtccaacaat aaacgagccg atcttgtggt gagatcggct
     2521 cgattattgt tccgcttgac aaaccaagca ccctcaagta tgatctgtac acaattctgt
     2581 acagagagca agtatgccca ccaaaaccat tcccatcacc gaagcgcgcg ccaatctgcc
     2641 gcagatcatc aaggccgccg acgagcggct cgatcgaacc gtcatcacgt cgaacggcaa
     2701 accggccgcg gtcatcatga gttacgacga atacga
//
LOCUS       DASRTJ010000437         7313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_276708,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000437 DASRTJ010000000
VERSION     DASRTJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276708"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1340)
                     /locus_tag="VFF70_08930"
     CDS             complement(93..1340)
                     /locus_tag="VFF70_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44858.1"
                     /translation="MRFRSGMLMTIGLMIFLAGCGSRPLLAKASFTPDTVSPNGDNQA
                     DATLVHYELSSDANLSIYFESRAGQKYFFRQDQFRSTGAYDVLFGGVIDGQLLPDGQY
                     QWIVEAKDQAGRSQSLAGTLTITGGDSTAPQFTTFTVLPKVFTPNQDGISDRTTINVG
                     LNKPATLLVSVQNILCNGSTAPPADRPDLECTPYLLAEKQGRRAVGDSGVHEFDYDAG
                     VDLGANPPPDGTYIITARAEDSVGQVTVTSDTLTIQAGGIPQAEIADGTVNFSADSLL
                     VGQTLYFTLTVDNYGPVAIRTSGPNPGYIYEMDQNYNVPGFAEESGAWRVGIDFDTSE
                     RNYPFRWSVGGPNDLIVQEVNGVKYYYLPPGKQAIIAGGIHLTEKPPRDPLYFWAGLI
                     HEDVEIRSINNRVDPHFITIDKP"
     gene            1477..2805
                     /locus_tag="VFF70_08935"
     CDS             1477..2805
                     /locus_tag="VFF70_08935"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZY44859.1"
                     /translation="MHTYREALDILNRFINYERSQPASYSPETLNLDRVNLLLDRLGR
                     PDRQFRSIHIAGTKGKGSTAAMIESVLRAAGYRTGLYTSPHLHTFRERMRVNNELISR
                     ENFATTFNLIEAHVNAIEGITWFEIVTALAFTYFAQSKIDVGVIEVGLGGRFDATNVL
                     QPIVSVITSLSLDHMNLLGNTLEQIAFEKAGIIKPKIPVVSSPQRPEALAVIEKTAQE
                     RNAPLVVVGKEVTFEILTSSIDGQEFSVTAQNGAIHSYKIPLLGAHQASNAATAITTL
                     KVAEQDGLSITHEAIASGLTKVKWPGRLEILSRDPLLVVDGAHNADSAQKLAQSLHDI
                     FGDQTWTLIIGISADKDIPAILDALFPIASRIIVTRAHNVRAADLESLAEKVVQHNLQ
                     PSITATVQDAIDLALNDHNAIIVTGSLFTVADAREAWFDRSGLSLPEKDE"
     gene            2986..5496
                     /gene="lon"
                     /locus_tag="VFF70_08940"
     CDS             2986..5496
                     /gene="lon"
                     /locus_tag="VFF70_08940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZY44860.1"
                     /translation="MQFQTWMNEAAMSELNPQAATEWFKPAMRPRTVDGTLEAPLLPV
                     RETVLFPHMITPLFVGRDRSIKAIEAAQANNESLIVVTQRDPELEDPRPEELYTLGTQ
                     VLIGRTLRMPDGTTSVLTQGQRRAQIIEFTQIEPYLRARVHLIDDPITKPPATEALMR
                     AVLALFEKVIHLNRALPEDAYVYAMNVTEPGWMADLVASTLDLELPIRQEVLEILDPA
                     SRLQRLSILLAKELDVLELEDQIHSRVQREVDRSQREYYLREQVKAIQTELGEGDLAA
                     QEISELREKINAKTFPDEIKARAEKEIVRLSQMPAMAPEVGIIRTYLDWLIELPWTEA
                     SEDNLDVAHAAEVLDKQHYGLPKAKERILEYIAVRKLAAEKMRSPILCFVGPPGTGKT
                     SLGKSIAEALGRKFVRVSLGGIRDEAEIRGHRRTYIGAMPGRIIQTLRRAEKINPLFM
                     LDEIDKVGNDFRGDPSAALLEALDPEQNFGFSDHYLEVAYDLSKVLFVTTANSLDTIP
                     PALRDRMEVIEFSGYIEEEKLAIAKHFLIPKQLGEHGITADQLRLNDETLRSIIREYT
                     YEAGVRNLDREIANIARKTARKVAESKRFSHQITPALLDKFLGPPKYSFGRSEAKDEI
                     GVATGVAWSEGGGDILPIEVTLMQGKGGLTLTGQLGDVMQESIQAAMSYARSRADQLH
                     IETNRFDKTDVHVHAPEGGIPKDGPSAGITVATALISAFTEQPVRREVAMTGEITLRG
                     RILPIGGLKEKAMAAHRAGIKTLLVPKKNEKDLVEIPKKIRDDLKIILVEHMDEVLTA
                     ALLPKAKKTPDARHGQPRKKSGLRGVKSKQARKPTRKN"
     gene            6030..6521
                     /locus_tag="VFF70_08945"
     CDS             6030..6521
                     /locus_tag="VFF70_08945"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZY44861.1"
                     /translation="MYELIKFLHVASVFLFLLSHGASANVSFQLKNERKPERIRALLD
                     LSNWSFIGMGVGFLLLLITGIIDSFAGPWADHNGWIWVSIGLLLAITVSMSVLGSMFY
                     TKVRAAVGVPPYKRSDQQALGETKSEEEIAKLLNTNQPIILSLIGFGGILIIAYLMMF
                     KPF"
     gene            6549..6935
                     /locus_tag="VFF70_08950"
     CDS             6549..6935
                     /locus_tag="VFF70_08950"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HZY44862.1"
                     /translation="MLNVSRIIYRISAWLFFVGVVVQVFLAGLVLLAPGVSSWANHAG
                     FGYFIGYVLLPLIVFAFVGRLTRAIKIQMGVLLLLYIVQLALIYLRTSVPIVSALHPV
                     NAMLLFWFSLRHARQAGSITLSTVST"
BASE COUNT         1818 a         1964 c         1900 g         1631 t
ORIGIN      
        1 ggttggcaag caaaaaatat tgaaacccta gttcaatcac gaattcacga attaatgaag
       61 cggccgcaag ccaatcgggt taccaaaacc ggttacggct tatcgatcgt gatgaaatgc
      121 ggatcgaccc ggttattgat cgacctaatc tcaacgtcct cgtgaatcag gccggcccaa
      181 aaataaagcg ggtcgcgcgg tggcttttca gtcaggtgaa taccgccggc aatgatcgcc
      241 tgcttgcccg gcggcaggta ataatatttc acgccgttga cttcttgcac gatcaaatca
      301 tttgggccac caaccgacca gcgaaacgga tagttgcgct cgctcgtatc aaaatcaata
      361 cccacacgcc acgcaccgga ttcctccgca aagcccggaa cgttataatt ttgatccatc
      421 tcataaatat agcccggatt cggaccgctc gtccgaatcg ccaccggtcc atagttatca
      481 accgtcaggg taaagtacaa cgtctgcccg actaataacg aatcagccga aaaattcacg
      541 gtgccatctg cgatttccgc ctgcgggatt cctccagcct gaatcgtcaa agtgtcagac
      601 gtaaccgtta cttgtccaac gctgtcttcc gcgcgcgcag taatgatata cgttccatca
      661 ggcggtggat ttgcgccaag atcaactccc gcatcataat cgaattcgtg gacaccggaa
      721 tcgccgactg cgcgtcggcc ttgtttttca gccagcagat atggcgtgca ctccagatca
      781 ggtcgatcag ccggtggcgc cgttgagcca ttgcacaaaa tattctgcac cgaaacaagt
      841 aaagtcgctg gcttattcaa gcccacattg atcgttgtgc gatcggagat accatcttgg
      901 tttggtgtaa agactttggg taaaaccgtg aaggttgtaa actgcggcgc ggtcgaatcg
      961 ccgcccgtga tcgtcaatgt gcctgctagc gattggctgc ggccagcctg atctttcgct
     1021 tcaacgatcc attggtattg tccatcgggc agcagctgcc cgtcgatcac accgccaaat
     1081 aacacgtcat acgcgccggt cgatcgaaat tgatcttgcc gaaagaaata tttctgcccg
     1141 gctcttgatt caaaatagat tgataaattc gcatcgctac ttaactcata atgcaccaag
     1201 gtggcgtcag cttgattgtc gccattgggt gaaaccgtat cgggcgtgaa agacgccttt
     1261 gccagcaatg gacgtgagcc gcagcccgcg aggaaaatca ttaacccgat tgtcatcagc
     1321 attcccgatc gaaatcgcac cgcgctcctc caagatcgaa cttccaattt acagttgggc
     1381 agtctaactg atggaggatg atgtgtcaag cgtatgcgtt tacggactcg ccaagatagg
     1441 ttacaatcgt gcaacttcat tcaattgaga tcatcgatgc acacttatcg cgaagcgctg
     1501 gatattctga accgattcat caactatgag cgatcgcaac cggcgagcta ttcacccgaa
     1561 acactgaacc tcgatcgggt caatctgtta ctcgatcggc tggggcgccc cgatcggcaa
     1621 ttccgctcga tccacatcgc gggcacgaaa ggcaaaggct cgacggcggc gatgatcgaa
     1681 tcagttttgc gcgcagccgg ttatcgcacc ggattgtaca cttcgccgca tctgcatact
     1741 ttccgcgagc gaatgcgcgt caacaacgaa ttgatctcaa gggaaaactt tgccacaacc
     1801 ttcaatttga tcgaggcgca cgtcaatgcg atcgaaggca tcacctggtt tgagatcgtc
     1861 acggcactcg ccttcactta tttcgcgcaa tcgaagatcg atgtaggcgt gatcgaagtc
     1921 ggcctgggcg ggcggttcga tgcaacaaat gtgctgcagc cgatcgtgtc ggtgatcact
     1981 tcactcagcc tggatcatat gaatttgttg ggcaacactc tcgagcaaat cgcttttgaa
     2041 aaagcgggca tcatcaaacc gaagataccg gtcgttagtt caccgcaaag gcctgaagca
     2101 ctcgcggtga ttgagaaaac cgcacaagaa cggaatgctc ctttggtcgt tgttggaaaa
     2161 gaagtgactt ttgaaatact gacctcttcg atcgatgggc aggaattctc agtgaccgct
     2221 caaaatggag cgatccattc gtacaagatt ccgttacttg gagcgcatca ggccagcaac
     2281 gcggcaaccg ccatcacaac tttgaaagtc gctgaacaag atggtttgtc gatcacgcat
     2341 gaagccatcg ccagcggctt gaccaaagtg aaatggccgg gtcgcctgga aattctcagc
     2401 cgcgatccac tgctcgtcgt cgatggggcg cataatgcag actcggcaca gaagttagcg
     2461 caatcacttc acgacatctt cggcgatcaa acgtggacac tgatcatcgg catctcggcg
     2521 gataaagaca ttcccgcaat cctcgatgcg ttgttcccga tcgcatcgcg catcatcgtc
     2581 acgcgtgcgc ataacgttcg cgccgccgat ctggaatctt tggcggaaaa agttgtgcag
     2641 cacaatttgc agccttcgat cactgccacg gtacaggacg cgatcgatct cgcgctgaat
     2701 gaccacaacg caatcatcgt caccggatca ttgttcacgg tggccgatgc gcgcgaagcg
     2761 tggtttgatc gatcggggtt atcgttgccc gaaaaagacg agtaagccat gatcaattat
     2821 cgatgtacaa tgatcaatgt gcaatgtaaa attttcaacg aaaaatgaga agcgaatggc
     2881 gatgatcgaa attttgcccc ttgctcctcg cgccatgcac catttgcaca tcagactgaa
     2941 tgtatgcgac aatagactta tcggagcaga atgagagcaa gcaatttgca atttcaaacc
     3001 tggatgaacg aagcggcgat gagtgaattg aatccgcaag ccgcaaccga atggttcaaa
     3061 ccggcaatgc gaccgcgcac agtcgatggc acgctcgagg cgccgctgct gcccgtgcgc
     3121 gagacggtgt tgttcccgca catgatcaca ccgttgttcg tgggccgtga tcgatcgatt
     3181 aaagcgattg aggcagccca ggccaacaat gaatcattga tcgtcgtcac gcaacgcgat
     3241 ccggaattgg aagacccgcg accggaagag ctatataccc tcggcactca agttttgatc
     3301 ggccgcacgc tgcgcatgcc cgatggcacg acgagtgtgt tgacccaagg tcagcgccgc
     3361 gcacagatca tcgagttcac ccaaatcgag ccttacctac gcgcgcgggt gcatctgatc
     3421 gatgatccga tcaccaaacc gccggccact gaggccttga tgcgcgccgt gctggcattg
     3481 tttgaaaaag tgatccatct caatcgggct ttacccgaag acgcctacgt gtatgcaatg
     3541 aacgtgaccg agccgggctg gatggccgat ctcgtcgcct cgacgctcga tctggaattg
     3601 ccgatccgcc aggaagtgct ggagatactc gatcccgcat cgcgcttgca acgtttgtcg
     3661 atcttgctgg cgaaagaatt ggatgtcctc gaactcgaag atcaaattca ctcgcgcgtg
     3721 cagcgcgaag tcgatcgcag ccagcgcgaa tattatttgc gcgagcaagt caaagcgatc
     3781 cagaccgaat tgggcgaagg cgatctggcc gcacaggaaa ttagcgagct gcgtgaaaag
     3841 atcaacgcca aaacttttcc agatgagatc aaagcccgcg ccgaaaaaga aatcgtgcga
     3901 ttgtcacaaa tgccagcgat ggcgccggaa gtcggcatca tccgcaccta tctggattgg
     3961 ctgatcgaac tgccttggac ggaagcgtcg gaagacaatc tggatgtggc gcacgccgcc
     4021 gaagttttgg ataagcagca ttacggtttg cccaaagcca aagagcgcat tctggaatac
     4081 attgccgtgc gcaaattagc tgccgaaaaa atgcgcagtc cgatcttgtg tttcgtcggg
     4141 ccgccgggca cgggcaaaac atccttaggc aaatcgatcg cggaggcgct tggccgaaaa
     4201 tttgttcgcg tgagtttggg cggcattcgc gacgaggccg agattcgcgg ccatcgccgc
     4261 acgtatatcg gcgcgatgcc gggccgcata atccaaacgc tccgccgcgc tgaaaagatc
     4321 aacccgctgt tcatgctgga cgaaatcgat aaggtgggca acgactttcg cggcgatcca
     4381 tccgcggctt tgttggaagc gctcgatccc gaacagaact ttggcttcag cgatcattat
     4441 ctggaagttg cttacgatct ttccaaggtc ttgtttgtca cgacggccaa ctcgctcgat
     4501 acgattcccc cagccctgcg cgatcgcatg gaagtgatcg agttcagcgg ctacatcgag
     4561 gaagaaaagc tggcgattgc gaaacacttc ttgatcccga agcagctggg cgagcacggc
     4621 atcacagctg atcagctgcg cctaaacgat gagacgctac gatcgatcat ccgcgaatat
     4681 acttatgaag caggtgtacg caatctcgat cgggagatcg ccaacatcgc gcgcaagacg
     4741 gcgcgtaaag ttgccgaaag caaacgcttc agccatcaga tcacaccagc cctgctggat
     4801 aaattcctcg gaccgccgaa atattcgttt ggccgcagcg aagcgaaaga tgagattggc
     4861 gtcgcaacag gcgtggcctg gtcggaaggc ggcggcgaca ttctgccgat cgaggtgacg
     4921 ttgatgcagg gcaagggcgg cttgacgctt accgggcaac tgggcgatgt gatgcaggaa
     4981 tcgattcagg ccgccatgtc gtacgcgcga tcgcgcgccg atcaattgca catcgagacg
     5041 aacagattcg acaagaccga tgtacatgtg catgccccgg aaggcggcat tcccaaagat
     5101 ggcccatcag cagggatcac cgtggcgacc gccttgatct cggctttcac cgagcagcct
     5161 gttcggcgcg aagtcgcgat gacgggtgag atcactttac gcggccgcat cctgccgatc
     5221 ggcggattga aggaaaaagc gatggcagct catcgcgccg ggatcaagac cctgctcgtc
     5281 cccaagaaaa atgaaaagga tctcgtcgag attcccaaga aaattcggga cgaccttaag
     5341 ataattttgg tcgagcatat ggatgaagtg ctgactgcag cattgctgcc gaaagcaaag
     5401 aagacaccag acgcccgtca cgggcagcct cgtaagaaaa gcgggttgcg tggagtaaag
     5461 agtaaacaag cgcgaaaacc cacgcgcaag aattgaagtc gagcgaaata tattttgcaa
     5521 ttggcgtcga ggctttagcc ggtaaatgat tcactcaacc gcctaaaggc tcgactcctg
     5581 tctatattaa cctcacaatt gggataacaa tcctatgcca acgggaatgt aaaataggct
     5641 gagctggctc gatcgtgaaa gcgatcgccg tcaggtcgat cgatcccaaa ttgatctacg
     5701 ctggcacaca gctggcgtgg ctatatgtgt catgtggcag caactaccat tcaatcctaa
     5761 gagcatcaat cgaactttga ttgcgatctg aattattgtc ttgaccgttt gctcaatcgg
     5821 cactatcatc ccattcgatt taagacgcca gtcccacgac aatgggactg acaatgccct
     5881 aaagtatcca aatcaaatgc catgggaggc aatgtgagta gaactaagcg cgatctatca
     5941 gtatctagtt agcctgaagt aatccaaagc ccgatcggcc gatcagccga tcgggcttat
     6001 tttcaattta cacaataggg aggaatagca tgtacgaatt gatcaagttt ttgcacgtcg
     6061 ccagtgtatt tctgttcttg ctatcgcacg gcgcatccgc gaatgtttct tttcaattga
     6121 aaaatgagcg caagccggag cgcatccgcg cgctgcttga tttatcgaac tggtcgttca
     6181 tcgggatggg cgtcggtttc ctgctgctgc tcattaccgg catcatcgat agtttcgcgg
     6241 gaccgtgggc ggaccacaac ggctggatct gggtctcgat cggtttgcta ttggcgatca
     6301 cagtgtcgat gtcagtcctc ggctcgatgt tctacaccaa agttcgagca gctgtcggcg
     6361 tgccgcctta caagcgctcc gatcagcagg cactcggcga aaccaagagc gaagaagaga
     6421 ttgcaaaatt attgaatacc aatcagccga tcatactcag tttgattggc tttggcggaa
     6481 ttttgatcat cgcctacttg atgatgttca agccgttcta agcgcaaccc tttgattcag
     6541 gagacaacat gctcaatgta agccgcatca tttatcgaat ttcagcctgg ttattcttcg
     6601 tcggcgtcgt cgtgcaagta ttccttgcgg ggctggtgct ccttgctccg ggggtttcaa
     6661 gttgggccaa ccacgcaggg ttcggatatt tcatcggcta tgtgctgttg cccttgatcg
     6721 ttttcgcgtt tgttggccgg ttgaccagag cgatcaagat tcagatgggc gtgttgctgc
     6781 tgttgtatat cgttcagctc gcgctgatct atttgcgcac cagcgttccc atcgtatccg
     6841 cactgcatcc cgtcaatgcc atgctgctgt tttggttttc actcaggcat gcccgccaag
     6901 caggttcgat caccctgagc accgtgtcga cctgatttga ttctcacctc aagccgcgcc
     6961 atcctgcctc aaatgaccac actcgctcac attgaccaat cgaaaacagc cggagtgccg
     7021 ggcgaaaatt tgctcggcgc gtggcaatag atcgcgcgcg cgaagcgggc ttggggcatt
     7081 agccatagac ttccgaagtt ttcgcggaac atttcgaaag tttgtgatca atatgctata
     7141 cttcgcctcg atccaatatc agccgaggca agcgtagatg actgcatcgc ccgacacatt
     7201 ccccggacta acatctgccg aagcagccga tcgcatcaga cgcggactga caaataaatt
     7261 ccagccgcgt tccagccgat cgtatcgaca aatattcttc gacaacctca tca
//
LOCUS       DASRTJ010000438         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_330115,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000438 DASRTJ010000000
VERSION     DASRTJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330115"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFF70_08955"
     CDS             complement(<1..1131)
                     /locus_tag="VFF70_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HZY44863.1"
                     /translation="MKHPLKSSLLIVLITSLIFTGVALAHADLLRSDPVENSTLDIAP
                     SQINLWFSEPVEPAFSQITVLFEDGSTVDAGDTMVSSSDPTQVGVSLTDQRQGTYIVS
                     WRVLSSADGHITSGAFVFSVGKPINTSVGSESSSGATTSPLDMAARALTFIGQTALVG
                     VLIFRWLIWRPALKAAGLGDDVDDRAIKLTRRVVFVILGINAIGAVLMLAAQTQLVGD
                     TWLSTRVGRVWIGRAATLIALGVLSEDIAAVGRRDRGSIWLNLIIGWLGLQLLFLTTL
                     TSHSAAVTSPPILPFLSDWLHLIGTSLWIGALVQMALIVPQVVKDLNTEDRAWLWLKV
                     VVEFSIAGAFGVGLLLITGTYMSFLNVGNWPALVNTVYGQALL"
     gene            complement(1245..>1507)
                     /locus_tag="VFF70_08960"
     CDS             complement(1245..>1507)
                     /locus_tag="VFF70_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44864.1"
                     /translation="TRRGTYTVKLSGKIETTDVNTSIDIEETVDASTLQFPETQPLIS
                     DLQNKINDLSSQLGSIRGFAVAGLALAVIALVLAGSQLRKKS"
BASE COUNT          367 a          435 c          417 g          288 t
ORIGIN      
        1 caacaaggct tggccgtaaa cggtattgac caacgccggc caattgccga cattcaagaa
       61 actcatatac gtgccggtga tcaacaacag gccgacgccg aacgcgcccg cgatggaaaa
      121 ttcgacgacg actttcaacc acagccacgc acgatcttcg gtgttgaggt ctttgacaac
      181 ctgcggcaca atcaacgcca tctgcaccag cgccccgatc cacaacgacg tcccgatcaa
      241 atgtagccag tcggacaaaa agggaagaat gggcggcgaa gtcacggcag cgctgtgact
      301 ggtgagtgtc gtgaggaaca acaactgcaa gccaagccaa ccgatgatca ggttcagcca
      361 gatcgatccg cgatcgcggc ggccgacggc agcaatatct tcggacaaca cacctagtgc
      421 gatcaaggtg gccgcccgac cgatccacac gcgccccacg cgcgtcgaga gccacgtgtc
      481 accgaccagt tgcgtttgcg ccgctaacat caacactgcg ccgatcgcat tgatgcccaa
      541 gatgacaaag accacacgcc gcgtcaactt gatcgcacga tcatcgacat catcgccgag
      601 tccggcggct ttcagcgcgg gccgccagat cagccagcga aagatcaaca cgccgacgag
      661 cgcggtttgt ccgatgaagg tcaatgcgcg cgcggccatg tcgagcggtg aggttgtagc
      721 gccgctgctc gattcactgc cgaccgatgt gttgattggt ttgccgacgg aaaacacaaa
      781 cgcgccggat gtaatgtgcc catcggccga actcaacacg cgccacgaca caatgtaagt
      841 gccctggcgt tgatcggtta acgatacgcc cacttgggtc ggatcgctgc tggagaccat
      901 ggtgtcgcct gcgtcgacgg tcgatccgtc ttcaaacaag acggtgatct gagagaaggc
      961 cggttcaacg ggttcgctga accacaagtt gatttgcgat ggcgcgatgt cgagcgtgga
     1021 attctcgacg ggatcagatc gaagcagatc ggcgtgagct aatgccacgc cggtaaaaat
     1081 cagcgaggtg atgaggacaa tgagtaatga ggatttaaga ggatgtttca tgatcggtgt
     1141 aggggcgacg cattcgctta agaatcattg atcgcttgga gtattgttca gcctgcgatc
     1201 acgacccaat cgaagccacg cgcgaatgcg ctgccctact gcggctaact cttcttacgc
     1261 aattggcttc ccgccaagac caacgcgatc accgccagag ctaaacccgc cacagcaaaa
     1321 ccgcgaatcg aacctagctg tgaactcaaa tcattgatct tgttttgcag atcgctgatc
     1381 aagggctgag tttcgggaaa ttgcagcgta gacgcatcga ccgtctcttc gatgtcgata
     1441 ctggtattca catcggtcgt ctcgatcttg ccgctgagtt ttaccgtgta ggtgccgcgc
     1501 cgcgtcg
//
LOCUS       DASRTJ010000439         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_102408,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000439 DASRTJ010000000
VERSION     DASRTJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102408"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFF70_08965"
     CDS             complement(<1..944)
                     /locus_tag="VFF70_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZY44865.1"
                     /translation="MSRIIVSLDLETTGLDPQRDAILEIGAIKFRDDEVLDQFSSLIN
                     PARSIPPKITEITGLRDADVANAPSLFTILPGLKTFVRDLPIIGHNIAFDLGFLQKQH
                     LFADNLAIDTFELAGILIPHAERYGLGALAREVGIDLPATHRALDDAKVAHALYQKMF
                     ERACDIPLKTLQEIVKFADRLAWAPQVFFADALKAATRGSFSGGSIAAQLKAKGLAPK
                     GPGPMFTPTIDAKPLRPKAEATPIETDALAALLESDGAFDKTFDHFEHRPQQVKMLRA
                     VSDAFNEGQHLLVEAGTGTGKSIAYLLPAIHWAVQNGER"
     gene            1095..1466
                     /locus_tag="VFF70_08970"
     CDS             1095..1466
                     /locus_tag="VFF70_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZY44866.1"
                     /translation="MPKSRTEQMVDRLRDLQAGTPDCEASAVVSVDGLIMASSLPAGV
                     EEDRVSAMSAAMLSLGERIASELGRGTLDQVYIHGNSGYVILMSVGQDAVLTVLAREQ
                     AKLGLIMLDMRRAASDLEKLV"
     gene            1698..3305
                     /locus_tag="VFF70_08975"
     CDS             1698..3305
                     /locus_tag="VFF70_08975"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZY44867.1"
                     /translation="MPKYIFCTGGVVSSVGKGVTAASIGRVLKSRGVSVTMQKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLTKASNATTGQIYADVIGKE
                     RHGDYLGGTIQVIPHITNEIKKRIGQAAKSSSPDVVIVEVGGTVGDIESLPFLEAIRQ
                     MRKDVGRDNVLYIHVTWLPHIGATNELKTKPTQHSVHELRGIGINPDMIVCRSDFPVE
                     AAIREKIALFTDVEMRAVIPLVTTSVLYEVPLLLEEAGVGDFIIDRLNLNASAAPDMS
                     DWRGMVSEMRAHKEPLPIAIVGKYVELKDAYMSVRESLYHAGVAHDVDVQIDWILSED
                     LEKGRGLDRLEKAAGIVVPGGFGYRGIEGKIIAAKYAREHKVPYLGLCLGMQVMCIEK
                     MRSIVGTEEPNSTEFNHTTQFPIIDLLPDQRGIADMGGTMRLGSYPCALVPATQAARA
                     YGSALVHERHRHRFEFNNSFRELMQKNGVVFSGLSPDGHLVEIAELADHPFMVGSQFH
                     PEFKSRPNRPHPLFKAFVEAAAAQLDK"
     gene            3492..3797
                     /locus_tag="VFF70_08980"
     CDS             3492..3797
                     /locus_tag="VFF70_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZY44868.1"
                     /translation="MSRIKSDMITIGGIEDHVHLLVRFPTTLAIATLLKEVKGTSSHL
                     VTHEIAPTEFFKWQGAYGAFTVHKDGVERVAYYINHQKQHHSDKSLIDDWEKCEIEE"
     gene            3887..4528
                     /locus_tag="VFF70_08985"
     CDS             3887..4528
                     /locus_tag="VFF70_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44869.1"
                     /translation="MIQVRDVFQVKFGHIDQAVALFARLPRVSAGHATTDIHYHVLTD
                     ISGPMYTLVTELMVDNLSAWEKIRDESFASPDYAEWFKQFQLFVEEGRREYYTLEGEC
                     EDWSKPGVIVVREVYKALKWQIQPAIKLIQRYGALLADRGVGQRPRILTDASGPMFQI
                     VIEIETESLAAWEGSRRNLFKQPEFQVWFTQLLTQVRSGSHEFYRVENTITRS"
BASE COUNT         1094 a         1244 c         1335 g         1055 t
ORIGIN      
        1 cgctcgccat tttgtaccgc ccaatggatc gccggtagca gatatgcgat cgatttgccg
       61 gtgcccgtgc ccgcttcgac taagagatgt tgaccttcat tgaatgcatc cgatacggcg
      121 cgcagcatct tcacttgctg cggtcgatgc tcaaagtgat cgaaggtctt atcaaacgcg
      181 ccgtctgatt caagcagcgc ggcgagggcg tcggtctcga tcggggtcgc ttcagccttg
      241 gggcgcagcg gcttggcatc gatcgtcggc gtaaacatgg ggccggggcc tttgggcgca
      301 agccctttgg ctttcaactg cgcggcgatc gatccgccgg agaatgatcc gcgcgtggcg
      361 gcttttaacg catcagcgaa aaaaacctgc ggcgcccacg ccagccgatc ggcaaacttc
      421 acgatctctt gcagcgtctt gagcggaata tcgcacgcgc gctcaaacat tttttggtac
      481 aacgcgtgcg cgactttcgc atcgtccaat gcacgatgcg tcgccggtag atcgatgccg
      541 acttcacgcg ccaacgctcc aagtccatac cgttcagcgt gcggaatgag aatgcccgct
      601 aattcaaaag tatcgatggc cagattgtca gcgaagagat gctgcttttg caggaagcct
      661 aaatcgaacg cgatgttgtg gccgatgatc ggcagatcgc gcacaaaggt tttgaggccg
      721 ggcaggatgg tgaacagcga tggggcattc gccacgtcgg catcgcgcag gccggttatt
      781 tccgtgatct tcggcgggat cgatcgggcg ggattgatca gcgacgaaaa ctgatcgagc
      841 acttcgtcat cccggaattt gatcgcgccg atctcaagga tcgcatcgcg ttgtggatcg
      901 aggccggtgg tttcgagatc gagtgaaaca atgatgcgtg acaaggacat ccaatctagg
      961 cgcggctcag gccgcgcggg aagattatac catgcgcccg cagatttttt catgagtttg
     1021 tttgacttgc cttatgacct gtggtatgat tttgcgcgta ctcattggag gtattgatga
     1081 aaggaccttc ctctatgccc aaatcacgca ccgaacagat ggtggatcgc ttgcgcgatt
     1141 tgcaagctgg cacaccggac tgcgaagcgt cagcggttgt atcggtcgat ggcttgatca
     1201 tggcctcatc tctgccagcg ggcgttgagg aagatcgggt atctgccatg tccgccgcca
     1261 tgctctcgct gggtgagcgc attgccagcg agttaggccg cggcacgttg gaccaagtct
     1321 acattcacgg caacagcggt tacgtgattc tgatgtcagt cggtcaagat gcggtcttga
     1381 ccgtgctggc ccgcgaacaa gccaagttgg gcttgatcat gttggatatg cgccgcgcag
     1441 cgagtgatct tgaaaaactg gtctaacgga aggcaactga tgcgcagatc ggcatcgcaa
     1501 cctaattaaa tataaattat ctccggtggg gtgagtcgcc caaagtgact caccccaccg
     1561 ctctttttgg cagttagcag gtggcgtatc gcgtatggct tatcgatgat aattaatcac
     1621 atttgttttg gttttgcgat tagcgatatg cgatagcgat tcgccgacaa acattcaagg
     1681 tgaggaggac agacaacatg ccaaaatata ttttctgtac tggtggagtc gtatccagcg
     1741 tgggcaaggg cgtaaccgcc gcgtcgatcg gccgggtatt gaagagccgg ggcgttagcg
     1801 tcacgatgca aaaactcgat ccctatatta atgtcgatcc gggcacgatg agcccgtatc
     1861 aacacggcga ggtcttcgtc accgacgatg gcgctgaaac cgatctcgat ctgggccatt
     1921 acgagcggtt catcgatgag aatttaacga aggccagcaa cgccacgacc ggccaaattt
     1981 atgcggatgt gatcggcaaa gaacggcatg gcgattattt gggcggcacg atccaggtga
     2041 ttccgcatat caccaacgaa atcaaaaagc ggatcggcca agccgccaaa tcatcttcgc
     2101 ccgatgtcgt catcgtcgaa gtcggcggaa ccgtcggcga tattgaaagc ctgcctttcc
     2161 tcgaagcgat ccgccaaatg cgcaaagatg tgggccgcga taacgtcctg tatattcacg
     2221 tgacatggct gccgcacatc ggcgcgacga acgaactgaa gaccaagccc acgcagcact
     2281 cggtgcacga actgcgcggc atcggcatca acccggatat gattgtgtgc cgatcggatt
     2341 ttccggttga ggccgcgatc cgcgagaaga tcgcgttgtt taccgatgtc gagatgcgcg
     2401 cagtgattcc attggtcacg acctccgttt tatatgaagt gcccttgctg ctcgaagaag
     2461 ccggcgtggg cgattttatt attgaccgat tgaatctcaa cgcttcggcg gcccctgaca
     2521 tgtccgattg gcgcggcatg gttagcgaga tgcgggcgca caaagagccg ctgccgatcg
     2581 cgatcgtcgg caaatatgtg gagttgaaag acgcctacat gagcgtgcgc gaaagtttat
     2641 atcacgccgg cgttgcgcac gatgtcgatg tgcagatcga ttggattctg tcggaggatt
     2701 tggaaaaagg ccgcgggctc gatcgattgg aaaaagccgc cggtattgtc gtgccgggcg
     2761 gcttcggcta tcgcggcatc gaaggcaaga tcatcgccgc caaatatgcg cgcgagcata
     2821 aagtgcctta tctcggcctg tgcctcggca tgcaagtgat gtgcattgaa aagatgcgat
     2881 cgatcgtcgg cacggaagag ccgaacagca ccgagttcaa tcacaccacg cagtttccca
     2941 tcatcgacct cttgcccgat caacgcggca tcgccgacat gggcggcaca atgcggctgg
     3001 gcagctatcc ctgcgcgtta gtgccggcta cgcaagctgc acgcgcgtat ggctcggcct
     3061 tagtgcacga acggcatcgc catcgatttg agttcaacaa ttcttttcga gagttaatgc
     3121 agaagaatgg cgtcgtgttc agcggcctgt cgcccgacgg tcacttggtc gaaattgccg
     3181 aattagccga tcatccgttc atggttggtt cgcagttcca tcccgaattc aagagccgcc
     3241 cgaatcgacc gcatccgctg ttcaaagcgt tcgttgaagc ggcggccgcg cagctggaca
     3301 agtaaattcg gacttgcgat tgaaatcgca gcaacaacac cgcaaagtcg gccaacgccg
     3361 actgagtgag gatgtaaggt gcgcgctcct tatactcaac tgtatctgcc ttgcgtttgg
     3421 gcaacatggg atcgattgcc actgattaca ccgagcattg aaggccgcct gtatgtcgcc
     3481 attgccgcaa agtgtctcga attaaaagcg acatgatcac gatcggcggc attgaggatc
     3541 acgttcatct cctcgtgcgt tttcctacca cattagcgat cgcaacgctg cttaaagaag
     3601 tgaagggcac atcatcgcat cttgtcacac atgaaattgc gccaactgaa ttctttaaat
     3661 ggcagggggc atatggcgca ttcactgtac acaaagatgg agtcgagcgt gtcgcctact
     3721 acatcaatca ccagaaacaa catcactccg ataagagctt gatcgatgat tgggagaagt
     3781 gcgaaataga agaataaatc agccgacgca ggtcggcttc gcgtctttgt tggtgcagtt
     3841 tcaactgcta cctcgtatca agtataataa gcctcagggg ggaatcatga tccaagtccg
     3901 cgatgtcttt caagtcaaat tcggtcatat cgatcaagcc gtggcgctgt tcgcgcgcct
     3961 gccaagagtc tcggcgggac acgccaccac ggatatacat tatcacgtcc tgaccgacat
     4021 ctccggtccg atgtatacgc tggtaaccga actcatggtt gataatttaa gcgcatggga
     4081 aaagattcga gatgaaagtt ttgcctcgcc cgactatgcc gaatggttca agcagttcca
     4141 gttgttcgtc gaagagggtc gccgcgaata ctacacgctc gaaggcgagt gcgaagattg
     4201 gtcgaagcca ggcgtgatcg ttgtgcgtga ggtctacaaa gcgctgaagt ggcagattca
     4261 accggcgatc aaattgattc aacgttatgg cgcactgctg gccgatcgag gggtgggcca
     4321 gcgcccgcgc atcctcaccg atgccagcgg cccaatgttc caaatcgtca ttgagatcga
     4381 aaccgaaagc ctggccgcgt gggaaggttc gcggcgcaat ctattcaagc agccggagtt
     4441 tcaagtgtgg ttcacacagc tgctcactca agttcgatct ggctcacatg aattttatcg
     4501 cgttgaaaat accatcaccc gcagttaggc gccaacgcaa tcggtcatgt tataatcgca
     4561 atcaagaggc tggtcaccat gaccagcctc ttttgaatca gaaaggtttt gatttgtcat
     4621 ctgagcgaag cgacgcctag cggcccacct agtgcacagc actcagggca tgggcagaat
     4681 ctctgcgcta atcgatagag acccttcgct gcgctcaggg tgacgacc
//
LOCUS       DASRTJ010000440         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_102516,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000440 DASRTJ010000000
VERSION     DASRTJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102516"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFF70_08990"
     CDS             complement(<1..961)
                     /locus_tag="VFF70_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZY44870.1"
                     /translation="MRQTIRRSVILGIALGLIALLFGGWVPAVLAVVAGTLVGFLAPK
                     RDRASVEDRLIQAGVGGLIASAIIVLGGQLFDGGFLFAQGLGDAAKLTDHPADIVSSA
                     ALLAIILGPLSAIGMTWIQSRRDRKLVQRLNLIVLLVFLFIFPFVNQAAHLNWLASLI
                     FAEIYVLLALGLNIVVGYAGLLDLGYAAFFAIGAYTTGLLSSPTSPIHLQVNFWILIW
                     LAAGMAAVFGLLLGAPTLGLRGDYLAIVTLGFGEIVPVAFQQLIHITIKEPITCSVIP
                     AIQILFGGTPSPCPPNLVWLDKFNLTYGIQGIAPIIPPTLPIIT"
     gene            complement(981..2075)
                     /locus_tag="VFF70_08995"
     CDS             complement(981..2075)
                     /locus_tag="VFF70_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZY44871.1"
                     /translation="MRKLRRLLLTAIIIYIFLLLSPIAGFDLPHVVDTIKNQPALVMR
                     YLVIGIANGGIFAIIALGYTMVYGIIQLINFAHGDVWMMCTFMSLTVIAAMGTTSKSD
                     PGQIFITIVVALLVAIVFGGVLNAAIERVAYRRLRNAPRLAPLISAIGVSFILENLGQ
                     IWAGQGYIKFLTPYFGTSGGAAQKAYPDLIVRQDLFKDVLGLKGFPVDFLTKELFVIV
                     VAILLMVALRWFIANTRMGKAMRATSENRDAAKIMGIDIDRVISFTFLLGGGLAGAAA
                     VLNGLYVNTAWWLMGFQAGLRSFTAAVLGGIGNVTGAMLGGFLIGILTSLSDGLLDPS
                     WTRAWVFSVLVLVLVFRPTGLLGENVGQKA"
     gene            complement(2250..3524)
                     /locus_tag="VFF70_09000"
     CDS             complement(2250..3524)
                     /locus_tag="VFF70_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZY44872.1"
                     /translation="MKPTKLIVTLLVLASVLMGACTPAAGPAATTAPTGGTLKIVSSL
                     PLTGASLTQTDTIVKSIQLKLKQINNKVCGGNYTIVYESGDDASAALGAWDPAVETEN
                     ANKAVADASVIAYIGTFNSGAARLSIPILNKVSLVMVSPANTNPGLTKPGKGDPAKNE
                     PDVYYPSGKRNYVRVVAADDIQGAVAANWAKTLGVTSVYILDDADVYGKGVADVFNKS
                     AVALGLKVIAQESIDPKAADYKALMTKIAASNGGNAPDMIYVGMDVDHNAAQLLKDKV
                     STMGDNTKVKFMGPDGVQTQAFIDGAGKDVAEGAYATVGGIPNEQLPASAQQYLKDYA
                     AAYGATNEPYAVYGYEAINVVINALENVCKNGGNPTDRATVRDAVFATKNYSGVLGTW
                     SFDQYGDTSLTDMTGYVVKGGKYEAVGVLKAQ"
     gene            complement(3875..3997)
                     /locus_tag="VFF70_09005"
     CDS             complement(3875..3997)
                     /locus_tag="VFF70_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZY44873.1"
                     /translation="MDGAKSVLSAAALTYIAALAQSLLTLLYYVFLLTGSGRRR"
BASE COUNT          996 a         1080 c         1100 g          828 t
ORIGIN      
        1 gtgtaatgat cgggagagtg ggcggtatga tcggggcgat accctgaatg ccataagtta
       61 gattaaattt atccaaccat accaaatttg gcgggcacgg cgacggtgtt ccgccgaaca
      121 gaatctggat ggccgggatg actgagcagg taatcggctc tttaatcgta atatggatca
      181 gctgttgaaa ggcgactggt acgatctcgc caaagccgag cgtgactatc gccagataat
      241 cgccgcgcag ccccagcgtt ggagcgccca gcaacaagcc gaagacggcc gccatacctg
      301 ccgcaagcca aatgagtatc caaaaattta cctgaagatg gatggggctg gtcggtgagg
      361 acaacaaacc ggtggtataa gcgccgatgg cgaaaaatgc cgcgtagccc aagtccaaca
      421 agccggcata accgacgacg atattcagtc cgagcgccag cagcacgtag atctcggcga
      481 agatcaatga ggccagccag tttaaatgcg cggcttggtt tacgaacgga aagataaata
      541 gaaatacaag taagacaatg aggttgaggc gctgcaccaa tttgcgatca cgccggcttt
      601 ggatccaggt cattccgatc gcactcagcg gtccgaggat gatggcgagc aaagccgcac
      661 tgctaacaat gtctgccgga tgatcggtga gtttagctgc atcacccagc ccttgagcga
      721 ataagaaacc gccgtcaaag agctgtccgc ctaagacgat gatggctgag gcaatcaagc
      781 cacccacacc ggcctgaatt aatcgatctt ccacgctggc tcgatcgcgc tttggagcta
      841 agaagccaac caatgtgccg gccacgacgg ctaatacggc cggcacccac ccgccaaaca
      901 gcagagcgat cagccccaac gctattccga ggatgacact tcgacgtatg gtctgtctca
      961 cgttgtctcc ttaaaaagga ttaagctttt tgtcccacgt tttcgcctaa cagaccggtt
     1021 gggcggaaca ctagaaccaa caccaagacg gaaaagaccc aggcgcgcgt ccaagatgga
     1081 tcgagcaacc catcgctgag tgaggtcaga atgccaatca agaagccgcc caacattgcg
     1141 ccggtgacat tgccgatgcc ccccaggacg gccgccgtaa acgatcgcag cccggcctgg
     1201 aagcccatca gccaccaggc ggtgttcacg tacaatccat ttaagaccgc cgcggcaccg
     1261 gctaagccgc cgccgagcag gaaggtgaat gagatgaccc gatcgatatc aatgcccatg
     1321 atcttggccg cgtcgcgatt ttcgctggtc gcccgcatcg ccttgcccat gcgagtgttg
     1381 gcaatgaacc agcgcagcgc gaccatcaac agaatggcga caacgatgac aaacaattcc
     1441 ttggtcaaga aatcaacggg gaaacctttt agtcctaaga catctttgaa taagtcctgg
     1501 cggacgatca gatccggata cgctttctga gctgcgccgc cgctggtgcc aaaatagggc
     1561 gtaaggaatt tgatataacc ctgaccggcc cagatttgcc ccaggttttc taagataaat
     1621 gaaacgccaa tggcggagat caatggagcc aagcggggtg cattccgcaa gcgccgataa
     1681 gcaacgcgct cgatcgcagc attcagcacg ccgccgaaga cgatggcgac gagcaatgcc
     1741 acgacaatcg taataaagat ttgtcccgga tcgcttttgc tggtggtgcc cattgccgca
     1801 ataacagtca ggctcatgaa cgtacacatc atccacacgt cgccgtgggc aaagttaatc
     1861 agctggatga tgccgtagac catggtgtag ccgagggcga taatcgcgaa gataccaccg
     1921 ttggcgatgc caatgaccag ataacgcatc accaaagcag gctggttttt aatggtatcg
     1981 acgacgtgcg ggagatcaaa accggcgatg ggggacagca acaagaagat atagataatg
     2041 atagctgtga gcagaagacg tcggagctta cgcatcgatt gatttccctt caattctaga
     2101 gtgagccacg ttggtcttgt ttacccaacc tgtcgactag gtggaatcaa catctgctac
     2161 gagtagtaaa gctgtaggct aatgaaacaa acatttacaa agtggggcgg ctagtgccgc
     2221 cccactttgg tgtgtagcta gaactgagat tactgagcct tcaagacacc gactgcttcg
     2281 tatttgccgc ccttgaccac gtaaccggtc atgtcggtca agctggtgtc accgtactga
     2341 tcgaacgacc aagtgccgag cacgccgctg taattcttgg tggcgaagac cgcatcgcgc
     2401 acggttgcac gatcggtggg attgccacca ttcttgcaga cgttctcgag ggcgttgatc
     2461 acgacgttga tggcttcata gccatatacg gcgtatggct cgttggtcgc gccgtaggcc
     2521 gccgcataat ccttcagata ttgctgagcc gaggccggca gttgttcgtt ggggataccg
     2581 ccgacggtgg cgtatgcacc ttcagctaca tccttgccgg cgccgtcaat gaaggcctgg
     2641 gtctgaacgc cgtccggacc catgaatttg accttcgtgt tgtcgcccat ggtcgagacc
     2701 ttgtctttca acaactgagc cgcgttgtga tcgacgtcca tgccgacgta gatcatgtcg
     2761 ggtgcattac cgccattgct agccgcaatc ttcgtcatca acgccttgta atcagccgct
     2821 ttgggatcga tcgactcttg agcaatgact ttgaggccga gcgctacagc ggacttattg
     2881 aacacatcag cgacaccctt gccgtagaca tcggcgtcgt ccagtatgta gacgctggtc
     2941 acgcccaagg tcttggccca gtttgcggcc acggcgccct gaatgtcgtc agcggcgact
     3001 acacgcacat agttgcgctt gccgctggga tagtagacat ccggttcatt cttcgccgga
     3061 tcacccttgc cgggcttggt caagccgggg ttcgtgttcg cgggcgaaac cataaccagg
     3121 ctgaccttgt tcaaaatcgg aatcgacagg cgggcagcgc ctgagttgaa cgtgccaatg
     3181 taagcaatga cggacgcatc cgcaactgct ttgttggcgt tctcggtctc aaccgccggg
     3241 tcccaagcgc ccaacgcggc tgaggcatca tcgcctgact catagacgat cgtgtaattg
     3301 ccgccgcaga ccttgttgtt gatctgcttc agcttcaact ggatcgactt gacgatggtg
     3361 tcagtctgcg tcagggacgc accggttaac ggcaggctcg acacgatctt cagcgtgccg
     3421 ccggtcggcg cggttgtcgc ggcggggcca gccgccgggg tgcaggcacc catcaaaaca
     3481 cttgcgagta ctaacaacgt aacaatcaac tttgtgggct tcatgttttt ctcctcctag
     3541 agaaattaca cgattctgaa tgcctcttca ctcgtggatc aagtttaagc ggagaggctg
     3601 acacgacgag aagcgcagaa caaaagggga aggggggtta gccctgccac tcgtcaaagt
     3661 tcggtggatg gtgtacgaga acccaatcac ctccctgtat tgcctaatta aaaacgtacc
     3721 ccgcaataag gaatggcgag gtagtgctag tggcaaggat tgtattacgt gatgaatgga
     3781 ttgtcaagca gaatgatcgc cctgaaaaat aaaacccgat cggctcatca aagcgatcgg
     3841 gttttgggaa aaggttggaa actgtccgtt tagtttatcg acgccggccg ctgccggtga
     3901 gtagaaacac gtagtacaac aaagttaata gcgattgagc caaggcggcg atgtaagtca
     3961 gagcagcagc gctcaacaca ctcttggcgc catcgatctc agtc
//
LOCUS       DASRTJ010000441         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_163249,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000441 DASRTJ010000000
VERSION     DASRTJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163249"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFF70_09010"
     CDS             complement(<1..1109)
                     /locus_tag="VFF70_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY44874.1"
                     /translation="MRFLRSFQCLLIFALLLSACNTATPTAQPAAPSPMPQSSPVPVA
                     LPIATQSGYPLRPTATLFSTPEAAPGVFTDFNNGVTLQYPLDWTTGPSDRPDTLSWFY
                     SPDGSVYAVLFFEPYSSADDLQTAAKKIEESALSGLQELKIISAGTTQLTDGRDAWST
                     VATASRTDNSKLKANVTTTIFGSHTFSLLVFGSPSDFDNNTAGFTTLINALQLEQAAR
                     YGIPRDQAIFLAGGESRNPREYDPATTHGSGDKLVYSGLVSFDPHLNLVPELAATWEV
                     TNGITYTFRLRPDARFHNGRPVTTQDVIYSWERAADPKTNSDTVLTYLGDIVGVKEMR
                     DGKADHVSGLKAIDDHTLQVVIDQAKPYFLLKLTYP"
     gene            complement(1109..2521)
                     /locus_tag="VFF70_09015"
     CDS             complement(1109..2521)
                     /locus_tag="VFF70_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HZY44875.1"
                     /translation="MRYRLFCIGLIISLLLILIPIAANAQGSIVATNDRAKLTFPDSI
                     AFSVHLKSTTQIDRVVIEYGVDQLTCGTVIAEAFPTFTPGTDVDASWTWEMKQTGSQP
                     PGSHIWWRWRVTDKAGHETVTEQKSNVWLDDQHPWQTDSGSLLNLHWYSGGSTFGLEL
                     HDSAVKSLGDLAASTGLQLDAPTDLYIYADTQDMQAAVLYEPGWTGGLAYPDHNIVII
                     GIAPDQIDWGKRTEAHELTHVLVGHLTFSCLGDVPTWLNEGLAVYGEGGPESGAVAQF
                     KDAVKNDTLISVRALSGGFSEDPAKADLSYSESYSLVNFLVKQYGQDKMTSLLKALRD
                     GSTVDDGLKSVYSFDIDGFEDAWRSNIGAKPRSIDHASPTATPIATIVPTYVPVSGAP
                     LGPTISPTRDRPASTPISIAQAASTQNPTTTNLPAITSTTPNASPINTDVIVPIAIGL
                     VLIIGVIFIVISRRQQRMKL"
     gene            complement(2591..3445)
                     /locus_tag="VFF70_09020"
     CDS             complement(2591..3445)
                     /locus_tag="VFF70_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZY44876.1"
                     /translation="MSPTPNFQPPTSNFQNERQLREEIVRVGKLLYDRGLIVATDGNI
                     SARLDDRRILSTPSGLCKGLMATDQLSIVDLDGKRIGPSSPANRDLVPTSEMRMHLEA
                     YKRRSDIRGVVHAHPPTVVAASIAGIDLSQCLIPEVIVMLGLIPTTDYATPSSEENVH
                     AIRDLIVGHDAIILQRHGTLVVGSSPTDAYLKTETVDQMARVSMMLEVLGKSAPLPPD
                     QVAKLVKQRQNLGLSRSADEGDFCEHCNVCHLPNQHLVGATPSGRPEITDQQIREITL
                     RVLHDLGK"
     gene            complement(3563..3835)
                     /locus_tag="VFF70_09025"
     CDS             complement(3563..3835)
                     /locus_tag="VFF70_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZY44877.1"
                     /translation="MYIGKVYGTVVATIKHPTFDRHKLLLVERLTPEGDATAKYDLCV
                     DVVQAGVGDRVLVLDEGNSARQVLNQVPNGAVRAVIVGVIDRVDLE"
     gene            complement(3888..>4076)
                     /locus_tag="VFF70_09030"
     CDS             complement(3888..>4076)
                     /locus_tag="VFF70_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44878.1"
                     /translation="SIYRALSLALGPGVNKKDNGPKIRPTTACTPTRRFAPQRAGVQS
                     SRGFRLNMGFRQTSAPRG"
BASE COUNT          877 a         1091 c         1187 g          921 t
ORIGIN      
        1 ggataggtga gcttcaataa gaagtacggc tttgcttgat cgatcaccac ctgcagcgtg
       61 tgatcgtcga tcgctttcaa gccgctgaca tgatcggctt tgccgtcgcg catctctttc
      121 acgccgacga tgtcgcccag ataagtgagt acagtgtccg aatttgtttt cggatcagcg
      181 gcgcgttccc acgaatagat cacgtcttga gtcgtgacag gccggccgtt gtgaaaacga
      241 gcgtcgggcc gcagacgaaa cgtgtaggtg atgccgttgg tcacttccca agtggctgcg
      301 agttccggca caaggttgag atgcggatcg aacgagacca agccgctata caccagcttg
      361 tctccgctgc cgtgcgtagt agccggatca tattcgcgcg gattgcggct ttcgccgccc
      421 gcaagaaata tggcttgatc gcgcggaatg ccatagcgtg ccgcttgttc aagttgtaga
      481 gcgttgatca atgttgtgaa gcccgcagta ttgttgtcga agtctgaagg cgagccgaag
      541 acgagcaggc taaatgtatg actgccgaag atcgtggttg tcacattcgc tttcaacttg
      601 gagttatctg tacgtgacgc ggtggcaacc gtactccaag catcacgtcc gtcggttaac
      661 tgggtggtac cagcactgat gatctttagc tcttgcaagc cgctgagcgc gctttcctcg
      721 atcttcttcg ccgcagtttg taagtcgtca gctgaagaat aaggctcgaa gaacaatacg
      781 gcatacacgc tgccatcggg actgtaaaac caacttaaag tatcaggccg atcgcttggt
      841 ccggttgtcc agtccagcgg atactgcaag gttacaccat tattaaaatc ggtgaacacg
      901 cccggcgcag cctccggcgt tgaaaataat gtcgcggtag gccgcagggg ataaccagat
      961 tgagtcgcaa tcggtaaagc gacaggcaca ggcgatgact gcggcatagg cgatggcgcg
     1021 gcgggttgcg cggtcggtgt ggccgtattg cacgcactga gcagcaatgc gaatattagc
     1081 aagcattgaa acgatcgaag gaatcgcatt aaagtttcat cctttgctgt cgccgcgaaa
     1141 tcacgatgaa gatgacaccg atgatcaaca ccaggccgat cgcgattggc acgatcacat
     1201 ccgtattgat cggtgaagcg ttcggtgtcg tcgatgtgat tgcgggcagg ttggtcgtcg
     1261 taggattctg tgttgatgca gcttgcgcga tcgatatcgg cgtcgaagcc ggtcgatcgc
     1321 gcgtcgggct gatggtcggt ccgagcggcg caccggacac cggcacatac gtcggcacga
     1381 tcgtggcgat cggcgtggcc gtcgggctgg cgtgatcgat cgagcgcggc ttggccccga
     1441 tattcgatcg ccacgcatct tcgaagccat cgatatcgaa actataaacc gacttcaagc
     1501 catcatcgac cgtcgatcca tcgcgcagcg ctttcaacag cgaggtcatt ttgtcttggc
     1561 cgtattgctt cactaagaag ttgaccaggc tatacgattc gctgtaagac agatcagctt
     1621 tggccgggtc ttccgaaaag ccgccgctga gtgcgcgcac ggagatcagg gtgtcgttct
     1681 tgaccgcgtc tttgaactgc gcgacggcgc cgctctccgg tccgccttca ccataaacgg
     1741 ccaggccttc attcaaccaa gtgggcacat cgcccaggca gctgaatgtg agatgaccga
     1801 cgagcacgtg cgtgagttca tgcgcttcgg tgcgtttgcc ccaatcaatt tgatcgggcg
     1861 cgatgccgat gatgacgatg ttgtgatcgg gatacgcgag tccgccggtc caaccaggtt
     1921 catatagcac ggcagcttgc atgtcttgcg tatccgcgta gatgtagaga tcggtcggtg
     1981 catcgagttg cagtccggtc gatgcggcga gatcgcccag ggatttcact gcggaatcat
     2041 gcagctcaag cccgaacgtt gatccgccgc tgtaccagtg caaattcagc agactgccac
     2101 tgtccgtttg ccacggatgt tgatcatcca gccacacgtt cgatttttgt tccgtgacgg
     2161 tttcatgccc agccttatcc gtcacgcgcc agcgccacca aatgtgcgag ccgggcggct
     2221 gcgatccggt ttgtttcatc tcccatgtcc acgacgcatc gacatcggtg ccgggcgtga
     2281 aagttggaaa cgcttcggcg atgacggtgc cgcacgtcag ttgatcgacg ccatattcga
     2341 tcacgacgcg atcgatttgc gtggtgctct tgagatgcac gctgaaggcg atgctgtccg
     2401 ggaaggtaag cttcgcccga tcgttcgtgg cgacgatcga gccttgtgcg ttcgcggcga
     2461 tcggaatgag gatcaatagc aagctgataa tcagaccaat gcaaaacagt cgatagcgca
     2521 tgggagttcc tttcagattc gtgcgtcatt gcgaggagtg ccgcaggcat gacgaagcaa
     2581 tgacgtcggc tcatttacct aagtcgtgca agacgcgcag ggtgatctcg cggatttgtt
     2641 gatcggtaat ttcaggacga ccacttgggg tcgcccccac aagatgctga ttgggcaaat
     2701 gacagacgtt gcagtgttca cagaagtcac cttcgtcagc cgatcgactt aaccccaaat
     2761 tttgtcgctg tttaacgagc ttggccactt gatcgggagg cagcggcgcg ctcttgccca
     2821 ggacttccag catcatcgac acgcgtgcca tctgatcgac cgtctcggtt ttgagatacg
     2881 catcggtcgg gctgctgcca acgacgagtg tgccatgccg ctgcaaaatg atcgcatcat
     2941 gcccaacgat cagatcacgg atggcgtgga cgttttcttc gctcgacggg gtagcgtaat
     3001 ccgtcgtggg aatcagaccg agcatgacga tcacttcggg aatcagacac tgcgatagat
     3061 cgatcccggc gatgctggcc gcgacgaccg tcggcgggtg ggcatgcacc acaccgcgaa
     3121 tatccgatcg gcgtttgtag gcttcaagat gcatccgcat ttcggatgtc ggcacgagat
     3181 cgcgatttgc gggtgaactc ggtccaatgc gcttgccatc cagatcgacg atgcttaact
     3241 gatcggtcgc catcaggcct ttgcacaatc cgctgggcgt agaaaggatg cgtcgatcgt
     3301 cgagccgcgc ggagatgtta ccatcggtcg cgacgatcaa gcctcgatcg tatagtagtt
     3361 tgccgacgcg gacgatctct tcgcgcagtt gacgttcgtt ctggaaattg gaagttggag
     3421 gttggaagtt gggagttggt gacattgcaa atctaataaa cacagagaca cacacttgcg
     3481 gtaaagcacg cagtgcgtac cggaggcacc gagaaaaact ctgatataac tctgtgtcgc
     3541 cgtgtctcgg tggttgatta ctctattcca agtcaactcg atcgatcacg ccgacgatga
     3601 cggcgcgcac cgcgccattc ggcacttgat tcaacacttg ccgcgcggag ttgccttcat
     3661 ccagaaccag cacgcgatcg ccgacgccgg cctgcaccac atcgacgcac aggtcatact
     3721 tagctgtggc atcgccttcg ggcgtcaggc gttcgacgag cagcaacttg tgccgatcga
     3781 aggttggatg tttgatggtg gcaacaacgg tgccgtaaac tttgccgata tacatgatga
     3841 tggattagtg atgagtaatt agtgaacaac gtgtccaacg ccccgcttca cccgcgcggc
     3901 gcggacgtct gcctgaaacc catgttgaga cggaaaccac gcgaactctg aacgcccgcc
     3961 cgttgaggcg cgaagcgccg cgtcggtgtg caagcggttg ttgggcggat ttttggtccg
     4021 ttatcctttt tgttcacacc aggccctaat gcaagcgata atgcacggta tattga
//
LOCUS       DASRTJ010000442         8662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_352409,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000442 DASRTJ010000000
VERSION     DASRTJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352409"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VFF70_09035"
     CDS             <1..1235
                     /locus_tag="VFF70_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44879.1"
                     /translation="QAISRNVIASEAKQSQLMITEIASAQKDAPRNDALPSSSGELNH
                     RPILDAYAFILIWLFFGLAPSMLTWPAASNSHGILAQMPAFIIAAIGLDAIVTRVEHS
                     PLAKSRLLAGLLVIIILFIHSLHSIVDYFGTWANLSSVRAEYAANMTSTAQYFAQNPA
                     STPLVFSSGNVTHYNPWSATAFRLIVPVGYRNARWFDARSSFIFPRGGTDLMLVNIAN
                     DDAPAPLDNRLIEDLFPIVEPASLVSAYFSATDLVSSLNTRLITLTQAMVNWPSEIHL
                     DAPKFPIPFDDRIDLIGYDVRKSIVQPGKNIRLTTYWRAQNIGLRPLSIFVHVLNDRH
                     AIAAQWDGFTIDQHYVQAGDIIVQVHFIGLPPDFPEGAYDMEVGLYEAETGVRVPIKI
                     DEQSITDRVLLQSITVQK"
     gene            1268..1750
                     /locus_tag="VFF70_09040"
     CDS             1268..1750
                     /locus_tag="VFF70_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZY44880.1"
                     /translation="MSDTLEAIVGKRLTEKKLTLALAESCTGGLIGHRVTDVPGSSTY
                     FIGGIVAYSYEAKEKLLGVHHDTLYDHGAVSVETAQEMARGARRVLGTDIGISATGIA
                     GPGGGLPGKPVGLVYICLSARDFERTEKFVWHKDRAGNKMDSADAALQMLREYLEAHA
                     "
     gene            2180..2818
                     /locus_tag="VFF70_09045"
     CDS             2180..2818
                     /locus_tag="VFF70_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY44881.1"
                     /translation="MERAECYNSGAQGWRDRSRIPIESPAPRTSIVQDELALLGRARS
                     LDPDALVQIHDTYFTPIFRYVAFRVSDRSVAEDLTSEVFTRLLSALRDKNAPQNTLRG
                     WLYGVASRVVSDYHRQHYRAPEVELDESLASRELDPAEAVEKMMTHENLKRVMQELTE
                     EQQNVISLRFGYDMPIQVVARTLGKTEGAVKQLQARAIATLARKLSPELADE"
     gene            2822..3970
                     /locus_tag="VFF70_09050"
     CDS             2822..3970
                     /locus_tag="VFF70_09050"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HZY44882.1"
                     /translation="MDTRLEAKFIECLSALDQGEAIDQILARYPDDAAKLRPLLITAQ
                     SIPALRMEPSEAVKMKSRDAFLQQAAALRQSKRRVIGFVPRFVTSLTAAIVIIAIVGV
                     GAIAASGSALPGDPLYGLKRTIETARLALSPDILSRGALAAQFDQTRVSEVKALITAS
                     RSTHVEFGGTIESIQNNTWLVAGITVLLNSSTSVTGTPLTRSRAQVIGTSSSNGVAAT
                     SITIDANTPAPTPTPAPSPTIEPTPTPIVTPTLTITPTQPITPTIAPTPTPIEVEFSG
                     VVQTIGVQSWTINGTLVQVTSSTQIESGIGIGQNVSVKAVQHSGGQLVAIQISKIVEP
                     DTGGGDSPPTQEPAEPGATDKPEPTEIREPTESPEPTEQPEPTETPHD"
     gene            4155..4547
                     /locus_tag="VFF70_09055"
     CDS             4155..4547
                     /locus_tag="VFF70_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZY44883.1"
                     /translation="MKFRSIAVLLLLVLTASIVLTACNGGSSTPAPTTGAKTIAVDAA
                     EFAFTPNKFDAKVGDKLTFKITNKGTLDHSFVIAGPDGVVITRTDVKQGGSASLDFTP
                     TKAGAYQISCDVPGHKDSGMVGTLTITP"
     gene            4777..5763
                     /gene="corA"
                     /locus_tag="VFF70_09060"
     CDS             4777..5763
                     /gene="corA"
                     /locus_tag="VFF70_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZY44884.1"
                     /translation="MIHFLYRAGKNELMADLPLDQLTAALRNKSGLIWIDLDGEDPET
                     YRPILADTFGFHPLAIDDALVESHIPKLDDWGEYIYLVLHAVDFDRSIMDLDTHELDC
                     FVGPNFLVTHHTEPVRAIDRVRQACLRDKRHLLRGPDYLLFELADALISDFMPCIDDL
                     DETIDRIEDEVFAKPTTSTLNSIFTLKRATIHLRRILSPQREVLNHLARDDYHAIDAS
                     EQIYFRGVYDHLVRLVDINESLRDLIGGTLDTYLSVVSNRLNEVMKVLTIVTVLFMPL
                     TFLTGFFGMNFFGSTYEVASPTNSGLLLIAAITSMIGMPLLMWYWLKRRGWV"
     gene            5808..6701
                     /locus_tag="VFF70_09065"
     CDS             5808..6701
                     /locus_tag="VFF70_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZY44885.1"
                     /translation="MIAFVLSGGANRGPLETGALQSLVAHNIKADFVVGTSAGALNGV
                     YYAYDPTPAGMQKLADVWINTKVQEIFPNPNRLAMAWRVLTGRDSLVDGKNFLNTIQK
                     YLPGKTFADLKIPFYAVTADLLSAQMIVFGDDPSTELLEPVAASASFPIVLPPIEIDD
                     FQLSDGGVTAIVPIQAALDRGATELYVIDLEPVVAKSAPVRGVLNIAMQTLMTALRAQ
                     LITDLKVAIKAGMKVHHVNITAFPDLDLFDFSQAAQLVEVGRLAMDTYLNAPKPNVIR
                     PLSATVQSLPRVPRGGKAMRQ"
     gene            6794..8098
                     /locus_tag="VFF70_09070"
     CDS             6794..8098
                     /locus_tag="VFF70_09070"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HZY44886.1"
                     /translation="MRAVDIIEKKRDGGELTADEIDFFIGGFTRGEIPDYQAAALCMT
                     IYFKDMTYAETANLTLAMARSGTMLDLKDIAPIVVDKHSSGGVGDKVSLIVEPLVAAS
                     GLPTGKMSGRGLSFSGGTIDKLESITGFRVDLSQTEFRRLLKQHGIVLTGQSPDMAPA
                     DGKLYALRDVIGAVPSIPLIASSIMSKKIAGGADAMVLDVKCGTGAFMKTLEDARKLA
                     ETMVRIGHELMRTTIAVISDMNQPLGFAVGNALEVKEAIETLHGRGPHDLTEHCLTIA
                     SYMLQLGDKARSFDEGRALAEKLLGDGSAWRKFVEWIEAQGGDVEMIQHPDRLPLAPI
                     VREVRSSQSGYLQSINALEVGLTSVDLGAGRAKKGDPIDYAVGIVVHGKVGDRIETNR
                     PLFTIHANDQAKAQAAEQRLIAAIQYSHAPVVPLPLFYDVVK"
     gene            8110..8616
                     /locus_tag="VFF70_09075"
     CDS             8110..8616
                     /locus_tag="VFF70_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY44887.1"
                     /translation="MEIRVLTAADSAAYREQRLRALKEDPEAFGDSYAQSVQRPIEWF
                     AERIRSKVNGSFTLGAFDQDQLVGTVGFARALGDKFSHKGDIWGMYVALEFRRRGIGR
                     ALMVEAIARARSIAELDHISLGVMTTQTIARQLYTSLGFVPWGTETNAVRVSDRFIDE
                     EFMILKLS"
BASE COUNT         1987 a         2380 c         2372 g         1923 t
ORIGIN      
        1 aacaagccat ctcgcgcaac gtcattgcga gcgaagcgaa gcaatctcag ctgatgatta
       61 ctgagattgc ttcagcgcaa aaagatgcgc ctcgcaatga cgctttacca tcatcgtctg
      121 gcgagttaaa tcatcggcca atccttgatg cttacgcttt cattttgatc tggctgtttt
      181 tcggcctcgc cccgtcaatg ttgacctggc ccgcggccag caacagtcac ggcatcctcg
      241 cccaaatgcc cgcgttcatc atcgccgcta tcggactgga tgcgatcgtt actcgcgttg
      301 aacacagtcc gctcgcaaaa tcaagattgt tggctggtct tcttgtaatc ataattctgt
      361 tcatccactc actccattcc attgtcgatt acttcggcac ctgggcgaat ctatcgagcg
      421 tgcgcgctga gtatgccgcc aacatgacct cgaccgcaca atacttcgcg cagaatcctg
      481 catcaacgcc cctggtgttc agcagcggca atgtgacgca ctacaatccg tggagcgcaa
      541 ccgcctttcg cctgatcgtg ccggtcggtt atcgcaacgc ccgttggttt gacgcccgat
      601 cgagttttat ttttccacgc ggtggaaccg atttgatgtt ggtgaatata gccaacgatg
      661 atgcgcccgc gccattggac aaccgcttga ttgaagacct gttcccgatt gttgagcctg
      721 cgtccttggt ctctgcctat ttcagcgcaa cagatttggt gtcttcatta aatacccgct
      781 tgatcacgct gactcaagca atggtcaatt ggccgagcga gatacatctc gatgcgccga
      841 agttcccgat tccttttgat gatcgcattg acttgatcgg ctatgatgtc cgcaaatcga
      901 tcgtgcagcc aggcaaaaac attcggctga ccacttactg gcgcgcccag aatatcggac
      961 taagaccact ctcgatcttc gtgcacgtgc tcaacgatcg acatgcgatc gccgcgcagt
     1021 gggatggctt cacgatcgat cagcattacg tccaagcggg cgatatcatt gtacaagtcc
     1081 attttatcgg cctgccgcca gattttcccg aaggcgctta tgacatggag gttggtctgt
     1141 acgaagctga gaccggcgtc cgcgtgccga tcaagatcga tgagcagagc ataactgatc
     1201 gagttctgtt acaatctatt acagtccaaa agtaggggtg agcggccgct cgcccacgga
     1261 ggggatcatg tcagacacac tcgaagcaat cgtcggcaaa aggttgacgg agaaaaaatt
     1321 gacgctggcc ttagcggaga gctgcaccgg cggattgatc ggccatcgcg tgaccgatgt
     1381 gccgggcagc tcaacgtatt ttatcggcgg cattgtggca tattcgtatg aagccaaaga
     1441 gaaattattg ggtgtgcatc acgacacgct atacgatcac ggcgcggtca gcgtcgagac
     1501 ggcgcaggaa atggcgcgcg gcgcacggcg tgtcctcggc accgacatcg gcatcagcgc
     1561 gaccggcatc gccgggccgg gcggcggctt gcccggcaaa ccggtgggct tggtctatat
     1621 ctgcttatcg gcgcgcgatt tcgagcgcac ggaaaaattc gtctggcaca aagatcgcgc
     1681 aggcaacaaa atggattcgg ccgatgcggc cttgcagatg ctgagagaat atcttgaagc
     1741 acacgcgtaa gggatgaagg ctgaggacgg agggataaag tgtttgactc aaattaaaac
     1801 ggccaccctc cccaagaagc ggtcgcgctc ttcattaata tttgctacct ttccttttca
     1861 ttgctgctta ttccccgtta tgtagcaccc cgttactttt tcgcgctgcc tgcgacttat
     1921 tgattgtcat caaccgtatc taggaacatt cgcttgtcat gctgagtgca acgaagcaca
     1981 cttgcaccgc aatgcggagc agtgcaggtg tctcggaaaa ttgtgcgaga cccttcgcgg
     2041 agttaatcct gagcgaagcc gaagggctca gggtgacagc ctcaaaactg ttttgaagcg
     2101 caagatgatc aacgggcgcg gggtgttcaa gaaatcgtag tgaggcagga tcgagcatga
     2161 acacaacgcc agccgctaaa tggaacgagc agagtgttat aattcgggcg cacagggctg
     2221 gcgggatcga tcgagaattc cgatcgaatc tccagcgccg aggacttcca tcgtgcaaga
     2281 tgaactggcc ttactgggcc gcgcgcgctc actcgatccg gacgcgctgg tgcaaattca
     2341 tgatacttac ttcacgccga tctttcggta cgtggccttt cgcgtcagcg atcgatcggt
     2401 tgccgaggat ttgaccagtg aagttttcac gcgcctgttg agcgcgctgc gcgataagaa
     2461 cgcgccccag aataccctgc gcggctggtt gtatggcgtg gcctcgcgcg tggtgagcga
     2521 ttatcatcgc caacattatc gcgcacccga agtcgaattg gacgaatcgc tggccagccg
     2581 tgaactcgat ccggccgagg ccgtcgaaaa aatgatgact cacgaaaatt tgaagcgggt
     2641 catgcaggaa ctgaccgaag agcagcaaaa cgtgatcagc ctgcggttcg gttacgatat
     2701 gccgattcaa gtagtagcgc ggacactggg caagaccgaa ggcgccgtta agcaactgca
     2761 ggcgcgcgcg attgccaccc tagcccgcaa gttgtcgcca gagctggcag atgaataacg
     2821 tatggatact cgactcgaag caaagttcat tgaatgttta agcgcgcttg atcaaggcga
     2881 agccatcgat caaattctgg cccgttatcc ggatgacgcg gcgaaactca ggccgttgtt
     2941 gatcaccgcg cagtccatac cagccctgcg catggaaccc tccgaagccg taaagatgaa
     3001 atcgcgcgac gcgttcttgc agcaagcagc tgctctgcga cagagcaagc gccgcgtgat
     3061 cggttttgtg ccgcgcttcg ttacctcgtt gaccgccgca atcgtgatca ttgccattgt
     3121 cggggtcggt gctatcgccg cttctggctc cgccttgcct ggcgatccgt tgtatggttt
     3181 gaaacgcacg atcgaaacag cgcggctggc cctcagtccc gatattttat cgcgcggcgc
     3241 attggccgct caattcgatc aaacgcgggt gagtgaagtc aaagccttaa tcacggccag
     3301 ccgatcgacc cacgtcgaat tcggtgggac gatcgaatcg attcaaaaca atacgtggtt
     3361 ggtggccggc atcaccgtgc tcttgaatag ttcgacttca gtgacgggca cgccgctgac
     3421 cagatcgcgc gcacaggtga ttgggaccag cagctcgaat ggtgtggccg ccacttcgat
     3481 caccatcgat gcgaatacac cggcgccaac gccgacgccc gcaccatcgc caacgatcga
     3541 gcctacgccc acgccgatcg tgacaccgac actgacgatc actccgacgc aaccgattac
     3601 gccgacgatc gcgccaacgc cgacaccgat cgaagtagaa ttttcgggcg tggtccaaac
     3661 aatcggagta cagagctgga cgatcaatgg cacactcgtc caagtgacta gctcaacgca
     3721 gatcgagagt gggatcggga tcgggcaaaa cgtcagcgtc aaagcagtcc aacacagtgg
     3781 tgggcaatta gtcgcgatac agatttcgaa aatcgtagag ccggataccg gcggcgggga
     3841 cagcccaccc actcaagagc cagccgagcc gggggcaacc gacaaaccgg agccgaccga
     3901 aatacgcgag ccgacggagt cgccggaacc taccgaacaa ccagaaccca ctgaaactcc
     3961 gcatgactaa tgtggctaac accaagccca acagatcgtc acaacgccgc caagattggc
     4021 ggcgttgtgt tgtccaactg tatttagtca tcggcatggt tgattttcgt tcattgtaaa
     4081 gccctataca aaatatggta taatctcgcg cgttgtcgaa aatcatgtgc ttaatgaaag
     4141 tggagggttt tgcaatgaag ttccgaagta tcgctgtact gttgttactg gttttgaccg
     4201 cctcgatcgt tttgacggct tgtaacggtg gtagctcaac gcccgcgcca acgaccggcg
     4261 ccaagaccat cgcggtggac gccgccgagt ttgcgttcac gcctaataaa tttgacgcga
     4321 aagtcggcga caagttgact ttcaagatca ccaataaagg cacgctcgat cacagttttg
     4381 tgatcgccgg accggatggc gttgtcatca cgcgcaccga tgtgaagcaa ggcggcagcg
     4441 cttcgcttga tttcacgcca actaaagcgg gcgcctatca aatttcctgc gatgtgccgg
     4501 gtcataaaga ttccggcatg gtgggcacgc tgactattac accctaacga tcagcttaac
     4561 ctcggcctat ggacccttcg atcaaagatc gaggggtctt ttgtttttcc gattggccag
     4621 ctgtgtccaa cgtttgtctg caccgagtta taatatgcca tgcgacggtc tcgaataaaa
     4681 caccggcgcg ggtcaagccg acttgctttt attgccgtcc atgcgaaact tgaggtgcgt
     4741 cgaagcaggc cgccaatcac aaccaaaggt gtagcgatga tccatttttt atatcgcgca
     4801 ggcaaaaatg aactcatggc tgatctgccg ctggatcagc tcacagccgc cctgcgcaac
     4861 aagtccggac taatctggat cgatctggac ggcgaagacc ctgaaaccta tcgaccgatc
     4921 ctcgcggaca ccttcggctt ccatccgctc gcgatcgacg acgcgctggt cgaatcgcac
     4981 atccccaagc tcgatgattg gggcgaatat atatatctcg tgctgcacgc cgtcgatttc
     5041 gatcgcagca tcatggattt ggacacgcac gaactcgatt gtttcgtggg tcccaatttt
     5101 ctggtcacgc atcataccga gccggtacga gcaatcgatc gtgtgcgaca ggcgtgcctg
     5161 cgcgacaagc gccacctgct gcgcggcccc gactatctgc tcttcgaact cgccgacgcg
     5221 cttatcagcg acttcatgcc atgcatcgac gacctcgacg aaacaatcga tcggattgaa
     5281 gatgaagtct tcgccaaacc taccacctcc accttgaaca gcatcttcac gctgaaacgc
     5341 gccacgattc acttacgccg gattctctcg cctcaacggg aagtcttgaa ccacttagcc
     5401 cgcgatgatt accacgcgat cgatgcgtca gagcaaatct actttcgcgg cgtatatgat
     5461 catttggtgc ggctggttga cattaatgaa tccttgcgcg atctaattgg cggcacgttg
     5521 gacacctatc tatctgttgt gtcgaatcgg ctgaatgaag taatgaaagt attgaccatc
     5581 gtgacggtct tgttcatgcc cttaactttt ctgaccggct tcttcggtat gaactttttt
     5641 ggcagcacgt atgaagttgc gtcgccaacc aacagtggac tgctgttgat cgccgccatc
     5701 acctcgatga tcggcatgcc gctattgatg tggtattggc tcaagcggcg cgggtgggtg
     5761 tgaaattttc gagctaagcc agcatccata tcttccagga ggttagcatg atcgcattcg
     5821 tactcagtgg cggggccaat cgcggcccgt tggaaaccgg cgcactgcaa tccttggtgg
     5881 cacacaacat caaagccgat tttgtggtcg gcacctcggc cggcgcgttg aacggcgtgt
     5941 attatgcgta cgatcctacg cccgctggca tgcagaaact ggccgacgtc tggatcaata
     6001 ccaaagttca agagatcttc cctaacccca atcgcttggc catggcctgg cgggtgctga
     6061 ccggtcgcga tagtttagtg gacggcaaaa atttcttgaa taccatccag aaatatctac
     6121 cgggcaaaac gtttgccgac ttgaaaattc ctttctacgc tgtcacggcc gatttgctgt
     6181 cggcgcaaat gatcgtgttt ggtgatgacc catcgaccga gctattggaa ccggtcgcgg
     6241 ccagtgcctc cttcccgatc gtgctgcctc cgatcgagat cgatgatttt caattgagtg
     6301 atggcggcgt aacggctatc gtgccgatcc aggccgcgct cgatcgcggt gcgaccgaac
     6361 tctatgtgat cgatctggaa ccggtggtgg cgaagagcgc gccagtgcgc ggcgtattga
     6421 acattgcgat gcagacgttg atgactgccc tgcgcgcaca attgatcacc gatctgaaag
     6481 tcgcgatcaa agccggaatg aaggtgcatc atgtcaacat cacggcgttt cccgatctcg
     6541 atctatttga tttctcccaa gcagcccaat tggtcgaggt gggtcgcctg gcgatggaca
     6601 cttacttaaa cgcacccaag ccgaatgtga ttcgaccgct cagcgccaca gttcaatcgt
     6661 tgccgcgcgt gccgcgcggt ggaaaagcga tgagacagta actcatctta agcagattgt
     6721 aggtgatgtc atgctgctta cgcgggtgtg accattgcaa aaggcaagcc cattattctg
     6781 accgaaggtg tttatgcgcg cagtcgacat cattgaaaaa aaacgcgacg gcggcgaact
     6841 tactgccgat gaaattgatt tcttcatcgg cggcttcacg cgcggcgaga ttccggatta
     6901 tcaggctgct gcgttgtgca tgacgatcta tttcaaagac atgacgtatg ccgagaccgc
     6961 caatctcaca ttggcgatgg cacgatccgg cacgatgctc gacttgaaag acattgcgcc
     7021 gatcgtcgtc gataaacatt ccagcggcgg cgtgggcgat aaggtcagct tgattgtcga
     7081 gccgttggtc gccgcctccg gtttgccgac cggcaaaatg tcgggacgcg gcttgtcttt
     7141 cagcggcggc accatcgaca agcttgaatc gatcacaggc tttcgcgtcg atctatcgca
     7201 aaccgagttt cggcgcttgt tgaaacaaca cggcattgtg ctcaccggcc aatcaccgga
     7261 tatggcgccg gccgatggca agctctatgc actgcgtgat gtgatcggcg cggtgcccag
     7321 cattccactg atcgcctcca gtatcatgag caagaagatc gccggcggcg cagacgccat
     7381 ggtgctggac gtgaagtgcg gcacgggcgc gttcatgaaa actttggaag acgcgcgtaa
     7441 actcgccgag acgatggtgc gcatcggtca tgagttgatg cgcacgacaa tcgcggtgat
     7501 cagtgacatg aatcaaccac tgggctttgc cgttggcaac gcgctggaag tgaaagaggc
     7561 catcgaaact ctgcatggcc gcggcccgca cgatctcacc gagcattgcc tgaccatcgc
     7621 gagctatatg ctgcaattag gcgataaagc tcgatcgttc gatgaaggcc gcgcactcgc
     7681 cgaaaaatta ttgggcgatg gatcagcctg gcgaaaattt gtcgaatgga tcgaagcaca
     7741 gggcggcgac gtcgagatga tacagcatcc cgatcggttg ccgctggccc cgatcgtccg
     7801 tgaagttcga tcgagtcaat cgggttacct tcaatcgatc aatgcgctgg aagtcggact
     7861 cacttcggtc gatctcggcg caggccgagc gaagaaaggc gatcccatcg actacgccgt
     7921 gggcatcgtc gtgcatggca aagtcggcga tcgaattgaa accaaccggc cactcttcac
     7981 aattcatgcg aatgatcaag ccaaagccca ggccgccgag caaaggctaa ttgccgccat
     8041 ccagtacagt catgctccag tagtgccact gcctttattt tacgatgtcg tgaagtgagc
     8101 gccgcctaaa tggaaattcg tgtcctaact gcagctgatt cggcagctta tcgcgaacag
     8161 cgcttgcgcg cgttgaaaga agatccagaa gccttcggcg attcatacgc acagtcggtg
     8221 cagcggccga tcgaatggtt tgctgaacgc atccgatcga aagtcaatgg cagttttact
     8281 ttaggcgcgt tcgatcaaga tcaattggtc ggcacggtcg gctttgcgcg cgcgctgggc
     8341 gacaagttta gccacaaggg cgacatctgg ggcatgtatg tcgcgctcga atttcgccgc
     8401 cggggaattg gtcgcgcatt gatggtggaa gcgatcgcgc gcgctcgatc gatcgcggag
     8461 ctggaccata tttcactcgg cgtcatgacg acccaaacca tagcgcgtca gctttatacg
     8521 tcgctgggct ttgtgccctg gggtacggaa accaacgccg ttcgcgtgag tgatcgattt
     8581 atcgatgaag agtttatgat tttgaaattg agttaagcga ggagcaaaat gaatccgttt
     8641 gtcattatgg gcgcggcgtt cg
//
LOCUS       DASRTJ010000443         7078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_102705,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000443 DASRTJ010000000
VERSION     DASRTJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102705"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2308)
                     /locus_tag="VFF70_09080"
     CDS             complement(65..2308)
                     /locus_tag="VFF70_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY44888.1"
                     /translation="MADEIVQFSRRSFLKLSGATIGAAGLASLNWSSAKAAAQDSLTY
                     DKIVPTMCEQCVWRCGVWAKVRDGKIVKLDGNPFHPHSNGKLCARGQSGIMTTYDPDR
                     ITTPLIRAGNRGEGKFRRASWNEALDLVAQKMLDIKNQYGAEAMIFSSTHNLSQPLFE
                     NLLYAFGSPNYGTQRSLCFNAMVVAHLTTYGIEEPGRDYSTVKYIIYTGRNMLEAIST
                     SETSDLMNAIGRGAHIVVLDPRFTKTASKAEWLPIKPGTDLAFHLALLNVIVGENLYN
                     KAFIELNAIGFDELAAEVVKYTPEWAASITGLSADTIRRIARDFAAAGPDAMAHPGWR
                     TSNFINSFQTERAITVLNALVGNWERLLIDSGSEGGGIALGKPQQPPYPRITAQRLDG
                     VPWKYPLVPLKIGVFQEIRDSILTGKPYQAHGWFIARQNPVMSLPDRSKTLQALDKLD
                     FIVTVSIIPNDTEWYADVILPESSYLERYDPLNVVDNRVFLRQPVIDPIGDTHSALWI
                     YKQLGERLGLGDYFQYADEEDYINQQLAPLGITVDAFAERGCWSPTLAQKTAGERSSC
                     FVPQVLSSEEPVEVPFELKAYKLNTPSGKIEIASETLRKSNQPAVPTWIEPPAPASDK
                     FYLLSGKVGQHTQFATQNNLWLNQVYAENELWLNPKAASARGINNGEIVKVTSDVGSA
                     KVKCKVTEGIREDCVWMTQGFGHQSKGLKTAYGKGAADSDLHGTFTDPVSGGQALSQT
                     FVVVEKA"
     gene            complement(2372..2926)
                     /locus_tag="VFF70_09085"
     CDS             complement(2372..2926)
                     /locus_tag="VFF70_09085"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZY44889.1"
                     /translation="MTIIAPIFIGFLFGFMLHKGGMTRYHKIVNVFRFTDLAVLKFMM
                     SSLVVGMTGIYLLTDLGVIANIPVTPTYIVGNLFGGLLFGVGMATAGVCAAPVAAGSG
                     EGRLDYIVPGMLGMFFGALMFGLTYNQVYPALAKLANSGPVTLPDVLNVNHWLVIAIF
                     TVIALMLFYVVDRVLKMRVDKTQI"
     gene            complement(3031..3573)
                     /locus_tag="VFF70_09090"
     CDS             complement(3031..3573)
                     /locus_tag="VFF70_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZY44890.1"
                     /translation="MNNVVAYLRKPEWSPYVSGGALGLVTIISLVLSSKLLPAPTLIG
                     ASGAYENLIAPVGLAIDPKNTYFNFVMPAGITWAVWVLVGIFLGGLVSALLSGTFKLR
                     KMPDQQWESIFGKSIIKRWAIVFCAAALLEYAAGIAGGCTSGLAISGGVVLAPASFIF
                     IAGMFASGIVTAMVIYRKKY"
     gene            complement(3589..4593)
                     /locus_tag="VFF70_09095"
     CDS             complement(3589..4593)
                     /locus_tag="VFF70_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44891.1"
                     /translation="MMKRQSLLIILAGAIIVIGLAIGQSAMTQVSAAGIDPSTIGSAV
                     FDRPLHCGVKVPDGLAQCGSAASSCKNCHEAKGEDPVNAKGDWHTQHAFGDFCEFCHA
                     GNVQATDKTAAHQGLVQPLEDVKANCSSCHTDDYQPKAQTYATTLGVTIGTSGSSQPP
                     TNPSAPSAPAPQQPAQPAPIAAPTSSNEVIDYASSAAPVNGNVIAAIVLVLMIIAGAG
                     LVYWNEQRLQRLAIPKSTEGYVTFETDRPSSAKDQLGSATTTEPIGEVSPELAKLLPV
                     LRSLDPRTLRALRIILSDRKRGEEMLQSLSLINFTVLEEMKRLDKKELSLLLALAGES
                     "
     gene            complement(4609..4956)
                     /locus_tag="VFF70_09100"
     CDS             complement(4609..4956)
                     /locus_tag="VFF70_09100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZY44892.1"
                     /translation="MSQPHKSEIQMLHAEICQALADPIRILLLYQLDDRPQSVGELGE
                     RLEINQPTVSRHLKVLRERGMVTTERHGTTVMYSMADHRVIEALDLMRSVMTDNLDRK
                     RALVDMLIAVEQH"
     gene            complement(5221..6441)
                     /locus_tag="VFF70_09105"
     CDS             complement(5221..6441)
                     /locus_tag="VFF70_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY44893.1"
                     /translation="MIIQGQWLGTAYTISLKDSSLAVSIGAREIIVYDRAGRLYTCNF
                     DDHHHRRSFSGQILQKWSIDNLRQRRWLDSIEADALIDRAAQRMRELIGALNSPDWNW
                     IIEPDQPEQLIEYVLIIERGAQFDSEAAAWDAARFQKVYSPIGMLPPDQYLALVLEAT
                     QGCTFNSCAFCDLYQQPFKVKSPTEFERHIDAVRNYLGDSFLLRSRSIFLGAANALAV
                     PMARLQPLLEIAVKEFNPDGQHGIGLYAFLDALTGMHKTVSDYVTLRQLGLRRIYIGL
                     ETGHDPLLALVHKPGGSIDAIETVRTIKAAGINAGVIVMIGLGGDRFNDGHITDTAST
                     LNAMHLGSGDLLYFSDLVEKPHTPYPTLAEEQSIRALTSIEMIEQRQSIRAQLRFGND
                     QPKISTYDVREFVY"
     gene            complement(6445..6864)
                     /locus_tag="VFF70_09110"
     CDS             complement(6445..6864)
                     /locus_tag="VFF70_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZY44894.1"
                     /translation="MFQNILVAYDGSPHSERALATALELAKCTEGHVTLLNAYDKVPD
                     YLGEPNFQETLDRTVVAARDKMLIVLEQVREQGLTASMNVLEGPPADAILRVAETEKH
                     DLIVMGSRGLGLFQELLLGSVSERVVRHAKIPVLIVR"
     gene            complement(6871..>7078)
                     /locus_tag="VFF70_09115"
     CDS             complement(6871..>7078)
                     /locus_tag="VFF70_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44895.1"
                     /translation="VVKQQYCAAVRQDAKLIETRVYPNSDFIAMDNFRVVKQECALGL
                     ECSLDDHVHCVWAGAGTHDPFAES"
BASE COUNT         1545 a         1956 c         1930 g         1647 t
ORIGIN      
        1 gcgtaatgcg tatttcgtaa tgaatccctt acgcagtacg cattacgcac tgcgcttcat
       61 ttgctcacgc tttttccact acaacaaacg tctgcgacag cgcttgcccg ccgctgaccg
      121 gatcggtgaa ggtcccatgc agatcgctat cggccgcgcc tttgccatac gcggtcttca
      181 agcccttcga ttgatggccg aagccctgcg tcatccacac gcaatcttcg cgaatgccct
      241 cggtcacttt gcatttgact ttggccgagc caacatcgct ggttactttt acgatttcac
      301 cgttattgat gccgcgcgca cttgccgctt tcggattgag ccataattca ttttcggcat
      361 acacttgatt cagccataaa ttattttgcg tggcgaattg cgtgtgctgg ccgaccttgc
      421 cgctgagcaa atagaacttg tcgctcgctg gggcaggcgg ttcgatccag gtgggcaccg
      481 cgggttgatt cgatttgcgc agcgtctctg aagcgatttc aatcttgcca ctgggcgtgt
      541 tgagcttata tgcctttagt tcgaacggca cttccaccgg ttcttcggac gaaagcactt
      601 gcggcacaaa gcacgatgat cgctcgcctg ccgttttctg tgccagggtc ggactccagc
      661 agccgcgctc ggcgaacgca tcgaccgtaa tgcccagcgg tgccagctgt tgattgatgt
      721 aatcctcttc gtcggcgtat tggaagtaat cgccgagacc taggcgctcg cccagttgtt
      781 tgtagatcca tagggcgctg tgagtatcgc cgatcggatc aatcacaggt tgacgcagga
      841 acacacgatt gtcgaccacg ttgagtggat cgtagcgctc gagatacgac gattctggca
      901 ggatcacatc ggcataccat tcggtatcat tcggaatgat gctgacggta acaatgaaat
      961 cgagtttgtc gagggcttgc agcgttttcg atcgatcggg caagctcatt accggattct
     1021 gccgcgcgat gaaccagccg tgcgcttgat acggcttgcc cgttaagatg ctgtcgcgaa
     1081 tttcttggaa gacaccgatc ttcaaaggca ccagcggata tttccacggg acaccatcga
     1141 gccgctgggc ggtgatgcgc gggtagggcg gctgctgtgg tttgcctaaa gcaatgccgc
     1201 cgccttcgct accgctatcg atcaacaatc gctcccagtt tccaacgagc gcgttgagga
     1261 cggtgatcgc ccgttcggtt tggaaggaat tgataaagtt gctagtgcgc cagccgggat
     1321 gcgccatcgc atccggtccg gcggctgcaa agtcgcgcgc gatgcggcgg atggtatcgg
     1381 ccgataggcc ggtgatcgag gcggcccatt cgggcgtata cttcacaact tcggcggcaa
     1441 gttcgtcaaa tccgatcgcg ttgagctcga tgaacgcttt gttatacaga ttttcgccga
     1501 cgatcacatt gagcagcgct aggtgaaaag cgagatcggt gccgggtttg atcggcagcc
     1561 attccgcttt gctggccgtc ttagtgaagc gtgggtccaa gaccacaata tgcgctccac
     1621 ggccgatcgc attcatcaaa tcgctggttt cgctggtcga gatcgcctcc agcatgttac
     1681 gccccgtgta aataatgtat ttcacggtac tgtaatcgcg gcccggctct tcgatgccgt
     1741 aagtggtaag atgcgcgacg accatcgcgt tgaagcacag acttctttgc gtgccgtaat
     1801 tcggtgagcc gaacgcgtac aacaaatttt caaacagcgg ctggctcaaa ttgtgcgttg
     1861 atgaaaagat catcgcttcc gcgccatatt gatttttgat gtcgagcatt ttctgcgcga
     1921 cgagatcgag ggcttcattc cacgaggcgc gacggaactt gccttcgcct cgattgccgg
     1981 ctcgaatcaa cggggttgtg atccgatcgg gatcgtacgt ggtcatgatc ccgctttggc
     2041 cgcgcgcgca cagcttgccg ttcgaatgcg gatggaacgg attgccgtcg agtttgacga
     2101 tcttgccgtc gcgcactttg gcccacacgc cgcagcgcca cacgcactgc tcgcacatcg
     2161 tcggcacgat cttgtcgtag gtcagactgt cttgagcggc agctttcgcc gaactccaat
     2221 tgagactggc taaacctgcc gcgccgatcg tcgcgccgga gagtttcaag aaacttctgc
     2281 gtgagaattg aacaatttca tcagccatgt gaacatcctg atgattagta attagtggtt
     2341 agtatgtcct aactaattac taatcactaa tttatatttg tgttttatcc acacgcatct
     2401 ttaggacgcg atcgacaacg tagaacagca tcaaggcgat gacagtgaag atcgcaatca
     2461 ccagccagtg attgacattt aatacatccg gcagcgtgac gggaccggaa ttcgcgagct
     2521 tggccaacgc gggataaacc tggttgtacg tcaggccgaa catcaacgcg ccgaagaaca
     2581 tgcccagcat gccgggcacg atgtaatcca agcggccctc gcccgatccg gcggcgacgg
     2641 gcgcggcgca cactccggcc gtagccatgc ccacgccaaa caataagccg ccgaacagat
     2701 tgccgacgat gtaagtgggt gtaactggaa tgttggcgat gacgcccaga tcggtcagca
     2761 ggtagatgcc ggtcatgccg acgacgagtg aggacatcat gaacttcaac acagcgagat
     2821 cggtgaagcg gaacacattg acgattttgt ggtagcgggt cataccgcct ttgtgcagca
     2881 tgaatccgaa caagaagccg atgaagatgg gagcgatgat ggtcatagtg aattctccta
     2941 gtgtcattgc gagcggaccg aagcaatctc ccgatcgatg atacgattgc ttcgtcgcgc
     3001 ggtgcgctcc tcgcaataac gcgaaattat ttaatatttc ttccgataga tcaccatcgc
     3061 cgtcacgatg cccgaggcga acattccggc gatgaagatg aacgaggcgg gcgctaagac
     3121 cacaccgccg ctgatggcca agccgctggt gcagccgccc gcgatgccgg ccgcatattc
     3181 gagcagggcc gccgcgcaga atacaattgc ccagcgtttg atgatgctct taccgaaaat
     3241 actttcccac tgctgatccg gcatcttgcg caacttgaat gtgcctgaca aaagcgcaga
     3301 cactaatccg cccaagaaaa ttccgacgag cacccagact gcccaggtga ttccagcggg
     3361 catcacgaaa ttgaagtagg tattcttcgg atcgatcgcc aagcccacgg gtgcgatcaa
     3421 attttcgtat gcgcccgatg caccgatcaa tgtcggggca ggcagcagtt tgctgctgag
     3481 gacgagcgag atgatcgtca ccagtcccaa ggcccctccg gagacatagg gcgaccattc
     3541 gggtttacgc agataagcga caacgttgtt catggtgtat tctccatttt atgattcgcc
     3601 ggcgagtgcc agcagcaaag acaactcttt cttgtccagc cgcttcatct cttccagcac
     3661 tgtgaaattg atcaaactca gcgattgcag catctcttcg ccgcgtttgc gatccgagag
     3721 gatgatacgc agcgcgcgca gtgtgcgtgg atcgagcgat cgaaggactg gcagcagctt
     3781 cgccagttcg ggcgatacct cgccgatcgg ttcggtggtt gtggcagaac ccaattggtc
     3841 tttcgcgctc gacggtcgat cggtttcaaa agtcacatag ccttccgtcg atttcggaat
     3901 cgccaagcgc tgcagtctct gctcattcca atagaccaat cctgctccag cgatgatcat
     3961 cagcaccagc acgatcgccg caatgacgtt gccgttcacg ggcgcagcgc tcgatgcgta
     4021 atcgatcact tcgttcgatg atgtgggtgc agcgatcggt gcaggttgag ctggttgttg
     4081 tggtgcaggc gcgctgggcg cagaaggatt agtcggcggc tgtgatgatc cgctggtgcc
     4141 gatcgtcacg cccagcgtgg tggcatacgt ttgcgctttc ggttgataat cgtcggtgtg
     4201 gcaagaggag caattcgctt tcacatcctc taagggttgg accaaaccct gatgggctgc
     4261 ggtcttatcg gtcgcctgca cgttgcccgc gtggcagaac tcacagaaat cgccgaacgc
     4321 gtgctgcgtg tgccaatcac ccttggcgtt gacgggatct tcgcctttcg cttcgtggca
     4381 gttcttgcac gacgaggctg ccgatccgca ctgcgctaaa ccgtccggca ctttgacgcc
     4441 gcagtgcagc ggacgatcaa aaacggcgga gccgatcgtt gaaggatcga tccccgccgc
     4501 tgaaacttgt gtcatcgcgc tttgcccgat cgccaggcca atgacaataa tcgcccccgc
     4561 caatatgatc aaaagagatt ggcgtttcat cattttaatt ctccgtgact aatgctgctc
     4621 gactgcgatc aacatatcga ccagcgcgcg cttgcgatcg aggttgtcgg tcatgacact
     4681 gcgcatgaga tccagtgctt cgatgacacg atgatcagcc atcgaataca tgactgtcgt
     4741 gccgtgccgc tcggtggtaa ccatgccgcg ctcgcgcagc accttgagat ggcgggagac
     4801 cgtcggttgg ttgatctcca atcgctcgcc aagttcaccc acgctttgcg gtcgatcgtc
     4861 caattgatac aacaacaaaa tgcggatcgg atcggccagc gcctggcaga tttcagcatg
     4921 cagcatttga atttcggact tatggggttg tgacatttta ctattcctcc tatcctttat
     4981 tcttatatgc ggatgagcat atttacctgt gaaaaatata acgcaatgta acagaggcga
     5041 taagtgcaga ttgtcactgt taggctatta caccactcct taccgcgatg attttgctga
     5101 taggcgtaag caataaccga ttgtagataa aaaaagcgct gaagatcaat ttgatcttca
     5161 gcgcggaatt accttcattc ggattaatat gcactatgcc tactgcattg tgcactcatt
     5221 ttaatataca aattctcgaa cgtcgtatgt ggatatcttc ggctgatcat ttccaaagcg
     5281 cagttgcgcg cggattgatt ggcgctgttc aatcatttcg attgacgtca gcgctcgaat
     5341 ggattgttct tcggccaatg tcggataagg cgtatgcggc ttttctacca agtcgctgaa
     5401 gtacaacaag tcgcccgagc ccaaatgcat cgcattcagc gttgaggccg tatcggtgat
     5461 gtgaccgtcg ttgaatcgat cgccgcccag gccgatcatc acaatcacgc cggcgttgat
     5521 gcccgcggct ttgatcgtgc gcacggtctc gatcgcgtcg atcgatccgc cgggtttgtg
     5581 aaccagcgct aacaagggat cgtgtcccgt ttccaaaccg atgtagattc ggcgcaagcc
     5641 tagctgcctc aaagtaacgt agtcgcttac ggttttatgc atgcccgtca gcgcatcgag
     5701 gaaagcatac aggccgatgc cgtgctgccc atctggattg aattctttca ccgcaatttc
     5761 caacagcggc tgcaagcgcg ccatcggtac ggctaaagca ttggccgcgc cgagaaagat
     5821 cgatcggctt cgcagcagga acgagtcgcc cagatagttg cgcactgcat cgatatgccg
     5881 ctcgaactcg gtcggcgact tgaccttgaa gggctgctga tacagatcgc agaacgcgca
     5941 cgaattgaaa gtgcagccct gcgtcgcctc taacaccagt gccagatatt gatcgggcgg
     6001 cagcatgccg attgggctgt agactttctg aaagcgcgca gcatcccagg cggcggcttc
     6061 actatcgaac tgcgcgccgc gctcgatgat caacacatac tcgatcagct gctcaggctg
     6121 atcgggttct atgatccagt tccaatcggg ggaattgagc gcgccaatca attcacgcat
     6181 ccgctgcgcg gcgcgatcga tcagcgcatc cgcttcgatc gagtccagcc agcgccgctg
     6241 gcggaggttg tcaatcgacc acttctggag gatttggccg ctgaagctgc ggcggtgatg
     6301 gtggtcgtcg aagttgcagg tgtacaggcg gccggcgcga tcgtaaacaa taatttctcg
     6361 tgcgccgatg ctcacggcca gcgagctgtc tttcaggcta atggtatagg ccgtgcccag
     6421 ccattgacct tgaatgatca ttgatcaacg gacaatcaat accggaattt tggcgtggcg
     6481 caccacccgc tcgctgacac tgccgagcag caactcttgg aaaagtccca acccgcgcga
     6541 gcccatgacg ataagatcat gtttctctgt ttcggctacg cgcagaattg catcggcggg
     6601 cggaccttcc aagacattca tgctcgccgt caagccttgc tcgcgcactt gctccaacac
     6661 tatcaacatc ttatcgcgcg cggccacaac tgtgcgatcc aatgtttcct gaaaattcgg
     6721 ctcgccgaga tagtctggca ctttgtcata ggcgttcaag agcgtgacgt gcccttcggt
     6781 gcatttggca agttcgaggg ctgtcgccag cgcgcgttcg ctgtgcggcg agccgtcata
     6841 ggcgactaaa atattttgaa acataaacct ttaactttct gcgaatggat cgtgtgtgcc
     6901 cgcgccggcc cacacacagt gaacgtgatc gtcgaggctg cactccagtc ccaacgcgca
     6961 ttcctgcttg acgacgcgga agttatccat cgcgatgaaa tcggaattag gatagacccg
     7021 cgtctcgatc aacttggcat cctgccgaac ggcggcgcaa tactgctgct tgacgacg
//
LOCUS       DASRTJ010000444         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_254877,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000444 DASRTJ010000000
VERSION     DASRTJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254877"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1384
                     /locus_tag="VFF70_09120"
     CDS             239..1384
                     /locus_tag="VFF70_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44896.1"
                     /translation="MRCIFKLSGLVMLISIGLLARSSSVWADELNRLINGGLDGSYHP
                     QCTRIGGNSWDVVLCDPAHIDSSTTILWGNVQVPGGWSAWWRSPNADQTDPNYFNSYP
                     AYCPDKSTTPPNCVPWHAPDFRDTATDPQGRGPDRRVGGDNSQKLSSFSAVYAAGLFQ
                     TVSGLTPGTVLRFSVNLEAWSSSDNDPSISSGQASMNLRVGIDPTGGTNPNSADIVWS
                     AAQDSFDRFSVFSIESTALADHVTVFTYARPVLAVQHNDVYIDEASLIELNAALTPTV
                     SSTDTGPTATPTKTATARPTRTPIPTATPRPTITPTPTVSPTATITHVPTAIPSPTAT
                     RIPVLVVGSRGINQFWVAIGILISALVIAYIVMRLTSSGSAGVDRPN"
BASE COUNT          330 a          436 c          456 g          361 t
ORIGIN      
        1 cgtcaagccg cgccaaattt tcggcgatat tacgcgtgac gccgccgggc ttaatcgtaa
       61 tcgagccggg cacatcggtg gtggagagcg gcgatcgact caaccgcgtt ttgatatcga
      121 tcgccgcgga gccgatggca aaaatggatt tcatgcgaga catgatacgc gacaggcaca
      181 tctttggcaa atgtcaatcg aattgatttg atctataatc gtgagtgagg cacagaatat
      241 gcggtgcatt ttcaagctaa gcgggttagt gatgctgatc tcgatcgggc tgctggcgcg
      301 atcgagttca gtttgggccg atgaattaaa tcgattgatc aacggcggct tggatggcag
      361 ctatcatccg caatgcacgc gtatcggtgg aaattcgtgg gacgtggtgc tatgcgatcc
      421 ggcgcatatc gattcgagca cgacgatttt gtggggcaat gtccaagtgc ccggtggttg
      481 gtcggcgtgg tggcgatcgc cgaatgcgga tcaaactgat cccaattact tcaattccta
      541 tccggcctac tgtcccgata agtccacaac gccgccgaac tgcgtgccgt ggcatgcgcc
      601 ggattttcgc gataccgcca ctgatccgca gggccgcggg ccggatcgac gcgtgggcgg
      661 cgacaattct caaaaacttt cttcgttctc ggccgtgtat gccgcgggct tgtttcaaac
      721 agtgagcggt ctgacgcccg gcaccgtgct gcgcttcagt gtgaatctgg aagcgtggtc
      781 aagttctgac aatgatccat cgatctccag cggacaagcg tcaatgaacc tgcgcgtggg
      841 gatcgatccg accggcggca cgaatccgaa tagcgccgat attgtctggt ccgcggcgca
      901 ggacagcttt gatcggttct cggttttctc gatcgagtcc acggccttag cggatcacgt
      961 cacggtcttc acttacgctc gcccggtgct ggctgtgcag cacaatgatg tgtatatcga
     1021 tgaagccagc ttgatcgagc tcaatgccgc gctcacgccc acggtgtcga gcaccgatac
     1081 cgggccgacg gccactccga caaagacggc tacggccagg cccacccgca cgccgattcc
     1141 gacggcgacg ccgcgcccga cgattacacc gacgccgacc gtctcaccaa ctgccacaat
     1201 cacgcatgtg ccaaccgcga taccttcgcc gacagctacg cgcatcccgg tcttagtcgt
     1261 cgggtcgcgc ggcatcaatc agttctgggt ggcgatcggg atattgatca gcgcgctggt
     1321 tatagcgtat atcgtcatgc gcctcacgtc ctccggctca gcgggtgtcg atcgtccgaa
     1381 ctgatgaatc aagcggtgtc aataaagaaa tctttaccca tgggtaggcc attgtcttga
     1441 cagcctgaat agctcaggta taatcgcgga cgttgactct acttgcgatg aggtctgtcg
     1501 caagtttcat attctaaagg agaagcatga ttttgaagaa gtttgtgccc gttttattgg
     1561 ctttagccgc cgtagtgttg acc
//
LOCUS       DASRTJ010000445         1934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_102756,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000445 DASRTJ010000000
VERSION     DASRTJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102756"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFF70_09125"
     CDS             <1..485
                     /locus_tag="VFF70_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY44897.1"
                     /translation="WLNFWQQLKASGVKIDEDWNTAYYTDFSGSSGKGPRPIVVSYAS
                     SPPAEVVFANPAIDQPPTAAIVAPKTCFRQIEFAGILKGTKNRDLAEKWIDFMLGLDY
                     QQDIPLQQYVYPVNPQAKLPDVFVKWSVIAEQPANLAPDVIALNRDQWIQQWTDAVLK
                     "
     gene            564..>1934
                     /locus_tag="VFF70_09130"
     CDS             564..>1934
                     /locus_tag="VFF70_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZY44898.1"
                     /translation="MSFPRRFLFILPLAFLAIFFFNPLATIIARGLAPDGVIDLSSFG
                     TIVGSPFYLQTVWFTFWQAIVSTLLTIVFALPAAYVFARFRFRGKAIMRALTTIPFVL
                     PTVVVAAAFSALIGPRGWINEALKAILQVDYAPLDIQNTIWIILLAHIFYNFTIVLRI
                     VGGFWTNLDPQIESAARVLGANRWRTFREITLPLLKPALLAAALLVFIFDFTSFGVVL
                     ILGGPQFNTIETAIYRNTQITLNLPMATALTIVQLICTFIFISIYTRLQARASHPIDL
                     RSQRLTQRLPVTRSEKYFVVSVIGSMLVLLCAPLIALIAKSFVNDAGFTLDNFINLAA
                     NPRGSVLFVPPLTALSNSLVFASVTMFIALVIGVLAAYSSSTGRQKILEPFWMLPLGA
                     SAVTLGFGFLIGLPSLRSTWLIIPLAHSLVAFPFVVRSITPALRSIRSNLREAASIMG
                     ANPMRAW"
BASE COUNT          394 a          538 c          482 g          520 t
ORIGIN      
        1 actggctgaa tttctggcag caactgaaag cgagcggcgt gaagatcgat gaggattgga
       61 acacggctta ttacaccgac ttcagcggat cgagtggcaa agggccgaga ccgatcgtgg
      121 tgagctatgc ctcaagtccg cccgccgaag tcgtgttcgc caatccagcg atcgatcaac
      181 cgccaaccgc agcgatcgtc gcgccgaaga cttgcttccg gcagatcgag ttcgctggaa
      241 tcttgaaggg gacgaagaat cgcgatttgg ctgagaagtg gatcgatttc atgctaggtc
      301 tcgattatca acaagatatt ccactgcaac agtatgtgta tccggtgaat ccacaagcca
      361 agttgccgga cgtgtttgtg aagtggtcag tgattgcgga gcaacccgct aatcttgcgc
      421 cggatgtgat tgcgctgaat cgcgatcagt ggattcagca atggactgac gcagtgctca
      481 aataaagaaa attaaccgca gaggcacaga gttgttgaag ttttcttctc cgtgctctcc
      541 gtgcctccgt gattaagatc agcatgtcat tcccacgccg gtttctcttt atcttacctc
      601 ttgccttcct cgccattttc tttttcaatc cactggcgac gatcatcgcg cgcggcctcg
      661 cgcccgacgg cgtgatcgat ctttcatcct tcggtacgat tgtcggaagt ccgttctatt
      721 tacaaacagt ctggttcaca ttttggcaag cgatcgtttc aactttactc acgattgttt
      781 ttgcactccc ggcggcctat gtctttgcgc gatttcgttt tcgcggcaaa gcgatcatgc
      841 gtgccttaac tacgatcccc ttcgttttgc caactgtcgt cgttgccgcc gcgttctcgg
      901 ccctgattgg tccacgcggc tggatcaatg aagcactcaa ggcgattctg caggtcgatt
      961 atgcgccgct cgatattcag aacaccatct ggatcatttt attggcgcat atcttctaca
     1021 acttcacgat cgtgctgcgc atcgtcggcg gattttggac caaccttgat ccacagatcg
     1081 aatccgccgc gcgcgtgctg ggcgcaaatc gctggcgcac ctttcgcgaa atcacgcttc
     1141 cattattaaa gcctgcgctg ctcgccgccg cgctgctcgt tttcattttc gatttcactt
     1201 cattcggcgt cgtattgatc ttgggcggcc cgcagttcaa cacaatcgaa acggcgatct
     1261 atcgcaatac acaaattact ttgaatctgc cgatggcgac cgcgttgacg atcgtgcagt
     1321 tgatctgcac gttcatcttc atttcgatct acacccgcct gcaagcgcgg gctagccacc
     1381 cgatcgatct tcggtcccaa cggctcactc agcggctgcc cgtcactcga agcgaaaaat
     1441 atttcgtggt cagtgtcatt ggctcgatgc tggtattgct gtgcgcccca ttgatcgcgt
     1501 tgatcgcgaa atcatttgtg aatgatgccg ggtttacact cgataacttc atcaacctcg
     1561 ccgccaaccc acgtggatca gttttgtttg tcccgccgct gacagccttg agcaattcat
     1621 tggtctttgc aagcgtgacg atgttcattg cattggtcat cggtgtgctg gctgcctatt
     1681 cttcatcaac tggccgtcaa aaaatactgg aacctttctg gatgctgccg ctgggcgcat
     1741 cagctgtaac actgggtttc gggtttttga tcggattgcc aagcttgcga tcaacttggc
     1801 tgattattcc actcgcacac tcgctcgttg cgttcccgtt tgtcgtccga tcaatcacgc
     1861 ccgccctgcg cagcattcga tcgaatttgc gcgaagcggc atcgatcatg ggcgcaaatc
     1921 cgatgagagc ttgg
//
LOCUS       DASRTJ010000446         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_102998,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000446 DASRTJ010000000
VERSION     DASRTJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102998"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFF70_09135"
     CDS             complement(<1..712)
                     /locus_tag="VFF70_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44899.1"
                     /translation="MFELTFLGTSASAPSVRRGLSSQVVMHNEYRFMIDCGEGTQRQL
                     LKSGLGFKKLDKILLTHGHLDHILGLAGLMSTLMRWEAITELNLYGGRPGLDRVEDLI
                     NKIVLRDADAPVRVNYHELQPGVIFADASLELSAFPVTHRGNGNFGFLFREKAHRPFL
                     NDRAEALGVPFGPERKRLVAGESIQLADGRMIQPSDVLGAEVAGATLAIVGDASRTDT
                     LIEYVRKADALVIESTYLE"
     gene            complement(750..1262)
                     /locus_tag="VFF70_09140"
     CDS             complement(750..1262)
                     /locus_tag="VFF70_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZY44900.1"
                     /translation="MLFSPIILTGSYLLHLIATVVWIGGLVVIALVIQPIASKTVTDK
                     AALAQLLDGIHKRFQPLANLSLIVLILTGLVQMVASKFYKGFLAIDNTWSQAILLKHL
                     AVGLMIVFAGIMTFSIEPAMRRTAFMAANDLVDEKVAARLRQQQLRLTRLNLVFSILV
                     LIFTAIARAQ"
     gene            complement(1311..>3028)
                     /locus_tag="VFF70_09145"
     CDS             complement(1311..>3028)
                     /locus_tag="VFF70_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44901.1"
                     /translation="WLKRDWLVLLLYLIATIVMTYPVAFRLGGEWVASRDVDTYVKLW
                     DQWRFGQTIHLGLPLNFTRDIFFPTGIDLTFHSTAWTVVTLAWLLSPIFGPIGAYNFT
                     ILIAVFTTAYGGYLLIRSMVDHRSAAWLGGAVYSFIPYHIAHTGGHPDLVHLAGIPIA
                     ALLFIRALTTSSIRSALGTALMLGFVALTSLYIMDFALITLVPLFIFLAFQQQRWRQR
                     DFWKVTILFSVATAIALSVRLFPLFQSGNALSAAIEDKYEAATLQTDLLSFGVPSPFN
                     PFFAPYVERIANQFPMNHKWPSYLGLVASVMGLSALTWKKRRGLTWMWAATGIIFGVM
                     ALGTVLRFNGTVYDNITLPLAYLEWLPPIRAIGRPDYFVLGLALPLAVGAGLGFDRWL
                     VAVGDRRRAKTLLIIGTTCFMLFEYWNGIYPGFTSTPNPFYTQIANEPDEFGLIDLPM
                     GRFNSKEYMYDQITHQRPIVEGLIGRTPLEAFTYIDNNPLLERWHTGTPLDCQETDAA
                     TIKHSLDQLATDNFRYIIVHQTDGTVPDTFSGYLTSQPVYHDDSITAYSIADLQTYPP
                     CTSTP"
BASE COUNT          792 a          808 c          820 g          608 t
ORIGIN      
        1 cctccaagta agtcgattcg atcaccagcg catcggcctt gcgcacatac tcgatcaaag
       61 tatccgtgcg actggcatcg ccgacgatgg cgagtgttgc cccggccact tcggccccta
      121 atacatctga tggttgaatc atgcggccgt cggctaattg gatcgattca ccggcgacca
      181 agcgcttgcg ctccggtccg aaaggcacgc ctaacgcctc agcccgatcg ttcaggaaag
      241 gtcgatgcgc tttctcgcga aacaaaaaac caaaattgcc gttaccacga tgcgtaactg
      301 gaaacgccga caattcaagt gaagcatcgg caaagataac gccgggctgc aactcatgat
      361 agttgaccct gactggtgca tcggcatcgc gcagcacgat cttgttgatc aaatcttcaa
      421 ctcgatcgag tccgggccgc ccgccgtaca aattcaattc cgtgatggct tcccagcgca
      481 tcagcgtgct catcagaccg gccaagccca gaatatgatc gaggtgcccg tgcgtcagca
      541 gaatcttatc caatttctta aagccgagtc cgcttttcaa taactggcgc tgcgtgcctt
      601 cgccgcagtc gatcatgaag cgatattcat tgtgcatcac cacttgcgac gacagcccgc
      661 gtcgaacgga cggggctgaa gcggaagtgc cgagaaaagt taactcaaac atagtgtacc
      721 gtatcgccga ctaaagtcgg cattccatgt cattgtgcgc gcgcaatcgc cgtaaagatc
      781 aacaccaaaa tactgaacac taaattcaag cgagtcaaac gtagttgttg ttggcgcaag
      841 cgcgcggcaa ctttttcatc caccagatcg ttggccgcca taaaggcagt gcgccgcatc
      901 gccggctcga tcgagaaggt cataattccg gcaaagacga tcatcagacc gacggccaag
      961 tgcttcaaca ggatcgcttg cgaccaagta ttatcgatcg ccagaaagcc tttgtagaat
     1021 ttgctggcga ccatctgcac gaggccggtc aaaatcaaaa cgatcaaact cagattggct
     1081 aacggctgaa agcgcttatg aattccatca agcaattggg ccagcgctgc tttatccgtc
     1141 acagttttcg aagcgatcgg ttgaatcacg agcgcgatga ccaccaagcc gccaatccac
     1201 accacggtcg cgatcaagtg caataaataa ctgcccgtca aaatgatcgg actgaataac
     1261 atggcgatga ttatacccga tggcttactt ctcgaaaagg cgcgaggtcg ctaaggcgtg
     1321 ctggtgcatg gcgggtaggt ttgcagatcg gcgatcgagt aagccgtgat cgaatcatcg
     1381 tgatagacag gttgggaagt taaataaccg gaaaaagtgt ctgggacagt tccatctgtt
     1441 tgatgcacga tgatataacg gaaattatcg gtagctagtt gatcaagtga gtgtttgatc
     1501 gttgctgcgt ctgtttcctg gcaatccagc ggtgtgccgg tgtgccaacg ctcgagcagc
     1561 ggattattgt cgatatacgt gaaggcttca agcggcgtgc ggccaatcag gccttcgaca
     1621 atcggccgct gatgagtgat ttgatcgtac atatattctt tggaattaaa gcggcccatc
     1681 ggcagatcga tcaacccgaa ctcgtccggc tcgttagcga tttgagtgta aaacggattg
     1741 ggtgttgagg tgaagccggg atagatacca ttccaatatt cgaataacat gaaacacgta
     1801 gtgccgatga tcagcagagt cttcgcacgg cggcgatcgc ccacagcaac cagccagcga
     1861 tcgaagccga ggccggcgcc gacggccaaa ggcagcgcca agcccagcac gaagtaatcc
     1921 ggtcgaccga tagcgcgaat gggcggcagc cattctagat aagctagcgg cagcgtgatg
     1981 ttgtcataga ctgtgccatt gaaacgcagg actgtgccca gcgccatgac gccgaatatg
     2041 attccggtcg ccgcccacat ccatgtcaag ccccgccgtt ttttccaagt caacgccgat
     2101 aagcccatca cgcttgcgac taacccaaga taagacggcc atttgtgatt catgggaaat
     2161 tgattggcga tcctctcgac gtatggcgca aaaaacggat tgaacggcga aggcacgccg
     2221 aacgataaaa gatcagtttg cagagtggcc gcttcatatt tatcttcaat cgccgctgag
     2281 agcgcattgc cagattggaa gagagggaat aggcgaacac tcagcgcaat tgctgttgcg
     2341 acactgaaga ggatcgtcac cttccaaaaa tctctctgcc gccaacgctg ttgctggaat
     2401 gctaaaaaaa taaacaacgg gaccagcgtg atcagcgcga agtccataat gtatagactg
     2461 gtcaaagcga cgaagcccaa catcaaagcc gtgcccagcg ccgatcgaat gctcgaggtg
     2521 gtcaacgcgc ggatgaacaa cagcgcggcg atcggaatcc cggccagatg aaccagatca
     2581 ggatgtccgc cggtgtgggc gatgtgatag ggaatgaagc tgtacaccgc gccgcccagc
     2641 caggcagccg atcggtgatc caccatcgat cggatgagca gatagccgcc ataggcggtc
     2701 gtgaacactg caatcaatat cgtgaagttg tacgcgccga tcgggccgaa gatcgggctg
     2761 agcagccagg ctaatgtcac gaccgtccac gcagtgctgt ggaaggtgag atcgatcccg
     2821 gttgggaaga aaatgtcacg ggtaaagtta agcggcaagc cgaggtggat ggtctgcccg
     2881 aaccgccatt gatcccacag tttcacgtaa gtgtccacat cgcgtgaagc gacccactcg
     2941 ccgccgagcc ggaaagcgac gggataggtc atgacgatgg tggcgatgag atacaacagc
     3001 aacacgagcc aatcgcgctt gagccagc
//
LOCUS       DASRTJ010000447         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_255185,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000447 DASRTJ010000000
VERSION     DASRTJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255185"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFF70_09150"
     CDS             complement(<1..304)
                     /locus_tag="VFF70_09150"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZY44902.1"
                     /translation="MKNFLGMWGPVAIWLAIIYAGSSVGHLPRAESKTIDSIIHRIGH
                     VAEFALLGALLLRAWRLQRSIAWRDVILVVIACGLYGFSDEWHQSFVAGRSSELSAV"
     gene            complement(349..1530)
                     /locus_tag="VFF70_09155"
     CDS             complement(349..1530)
                     /locus_tag="VFF70_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY44903.1"
                     /translation="MRIAMIGPFGLRPKGTMSVRALSMARALVKRGHAVKIVLPPWDH
                     PEDSGHAFEDHGVQIENITLPRIFAPLFQLVVMLRLIKHALVFKPDVIHCFKPKAYAG
                     LSAWWLWQLKRLRFSKVRLIIDSDDWEGAGGWNDRGQYSWLQKKFFAWQERWGLKHND
                     GLTLASRALQTIVWSMGLKPDKVMYVPNGIANADFGTREAEATSPRSVVYILLYTRFF
                     EYSVDRVLEVFRRVVDKVPQAHLLVVGKGFHEEEQQLLEQARQIGLHNQIDYIGWVDP
                     DALPGFFAQARVAIYPFDDTLINRTKCAVKLIDLLAAGVPVVADAVGQNMEYIRHNQT
                     GWLVTCGNVEAMAEAVVSAISDPNRAKSIGTAAMQDMYLRFDWDRLVESVETIYQGKL
                     L"
     gene            complement(1532..>2887)
                     /locus_tag="VFF70_09160"
     CDS             complement(1532..>2887)
                     /locus_tag="VFF70_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44904.1"
                     /translation="RDSFWLAVSEIPYAWTSLWGRFGYGQIPLSDSIYIGALIVTAVA
                     LIGLISAIIRRRSIFDAIQIKQLTLVIFSALLFAAALFGYMLTSTAGPMGRFYFPGLS
                     AFGVLMVVGLMELASSKLQVVRNNYSLLITRYFVPVIAFALALIAFFGYFVPAYAQPT
                     SIDPTTLKIEQPINVTFDGKIELLGAEIDRTSVQPGDPIHVTLYWRALQSIDQSYIEF
                     VHLIDGSGIIVAQRDTWPGRGMFPTVVWQAGETFADSLTLDVPEAAFAPNDAKIQAGL
                     FANDGTRLHALDASNSELDQDAVPLNQISILPRPGGYPNPIEANFGGKVDLLGYDMTS
                     RSILPGQALTVTLFWRSRSTFNADYSVFLNALRPSLRISAQDTGKPINGNFSTQAWQV
                     GQMITDVRVLQFPITAKVGQLDVEVGWFLPKSDRLDVLGDDGHVIDSRILLSPIRIRE
                     Q"
BASE COUNT          730 a          818 c          711 g          628 t
ORIGIN      
        1 acacggcgct caattctgaa cttcgaccgg cgacgaaact ttgatgccac tcatcgctga
       61 agccgtacag cccgcacgcg atgacaacga ggatgacatc ccgccacgcg attgatcgtt
      121 gtagacgcca cgcccgcaac agcaatgcgc cgagcagcgc gaattcggcc acgtgcccga
      181 tacggtggat gattgaatcg atcgttttgc tttccgcccg aggcagatga ccgactgatg
      241 atccggcata aataatggcc agccaaatcg cgaccggccc ccacatccct agaaaatttt
      301 tcattttatg aaaccgctaa agtttacctg aaatatctgc ccgtatattt acagcaattt
      361 cccttgataa atcgtctcga cggactcgac taatcgatcc caatcgaaac gcagatacat
      421 gtcctgcatc gccgccgtgc cgatgctttt ggcgcgattt ggatcactta tcgcgctaac
      481 cactgcctct gccatcgcct cgacattgcc acatgtcacc aaccagcccg tttggttgtg
      541 gcgaatatat tccatattct gcccgaccgc atcggccacg accggtacac ccgccgccag
      601 cagatcgatc aacttcacgg cgcatttcgt acgattgatc agcgtgtcat cgaacggata
      661 aattgccacg cgcgcttgag cgaagaaacc gggcaacgca tcgggatcga cccagcctat
      721 gtaatcgatt tggttatgca acccgatctg acgcgcctgc tcgagcaact gctgctcttc
      781 ttcgtgaaag cctttgccca ccactagcaa atgtgcttgc ggaactttat cgaccacgcg
      841 gcgaaaaact tctagcacgc gatcgacgct gtattcaaag aagcgggtgt acaacagaat
      901 ataaaccaca gatcgcggac tcgtcgcctc cgcctcccgc gttccgaaat ctgcattcgc
      961 gatgccgttc ggcacataca tcactttgtc cggcttcaag cccatcgacc acacgatcgt
     1021 ttgcaaagcc cgcgaggcta atgtaagtcc atcattatgc ttcaagcccc agcgttcttg
     1081 ccacgcgaaa aatttctttt gtagccagga atattgaccg cgatcattcc agccgcccgc
     1141 accttcccaa tcatcactgt cgataatcaa gcgcaccttt gagaaacgca gccgcttcaa
     1201 ctgccacagc caccacgccg agagtcccgc atacgctttg ggcttgaagc agtgaatcac
     1261 atcgggcttg aaaacgagcg catgtttgat cagccgcagc atcaccacca actgaaacaa
     1321 cggcgcaaat attcgtggca gagtaatatt ttcaatttgc acgccatgat cttcaaatgc
     1381 atgtcctgaa tcttcggggt gatcccacgg cggcaaaacg atcttcaccg catggccgcg
     1441 tttcactaac gcgcgcgcca tcgacagtgc gcggacagac attgtgcctt tgggccgcag
     1501 cccgaacggg ccaatcattg cgatgcgcat cttattgctc ccgaattcta attggactca
     1561 acaatattct tgaatcgatc acgtgaccgt catcgccaag cacgtccaat cgatcggact
     1621 tgggcagaaa ccagccgact tctacatcga gttgaccgac tttggcggtg atcggaaatt
     1681 gcagcacgcg cacatcggtg atcatttgac cgacttgcca ggcttgcgtc gagaaattgc
     1741 cattgatcgg tttgccggtg tcttgcgcgc ttattcgcag tgaagggcga agcgcattca
     1801 agaacacgga gtaatctgca ttgaatgtcg atcgactgcg ccaaaataaa gtgaccgtca
     1861 gtgcttgacc cggcaaaata ctgcgtgaag tcatatcata acccagcaga tcgactttgc
     1921 cgccgaagtt cgcttcgatg ggattcggat agccgccggg gcgtggaagg atcgagattt
     1981 gattcaaagg tacagcatct tgatcgagtt cactgttgct tgcatcgagc gcatgcaagc
     2041 gcgtgccgtc attagcaaat aagccagctt gaatcttagc atcattcggc gcgaatgcgg
     2101 cctcgggcac atcaagcgtt aacgaatcgg cgaaggtttc accggcctgc cacaccaccg
     2161 tcgggaacat gccgcgtccc ggccatgtgt cgcgttgggc aacgatgatg ccactgccgt
     2221 cgatcagatg cacaaactca atgtaagatt gatcgatcga ttgtaaggcg cgccaatata
     2281 atgtgacgtg gatcggatca ccgggttgaa cggatgtccg atcgatttcc gcgcctaaca
     2341 attcgatctt gccgtcgaac gtgacattga tcggctgttc gatcttcaga gttgtcggat
     2401 cgatcgatgt gggttgagcg tatgcgggaa cgaagtagcc gaaaaaggcg atgagcgcga
     2461 gcgcgaaggc gataaccgga acaaaataac gagtaatgag taacgagtaa ttgtttctta
     2521 caacttgtaa cttgctactt gctaactcca tcaatcctac aaccatcaac accccaaagg
     2581 ccgaaagacc cggaaagtag aagcggccca tcggtccggc ggtactggtc agcatataac
     2641 caaagagtgc ggcggcaaat aagagcgcgg agaagatgac cagagtgagt tgtttgatct
     2701 ggattgcgtc gaagatcgat cggcggcgga tgatggcgct gatcaaaccg atcaatgcaa
     2761 ccgccgtcac gatcagcgcg ccaatgtaaa tcgaatcaga cagcggaatt tggccgtagc
     2821 caaagcgccc ccacaaactt gtccatgcgt atggaatctc actgaccgcc agccagaaac
     2881 tgtcgcg
//
LOCUS       DASRTJ010000448         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_330854,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000448 DASRTJ010000000
VERSION     DASRTJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330854"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="tsaE"
                     /locus_tag="VFF70_09165"
     CDS             complement(<1..478)
                     /gene="tsaE"
                     /locus_tag="VFF70_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZY44905.1"
                     /translation="MSTLDPGAIEITSRSPDQTAQLGERLGRLLHGGEVICLAGQLGA
                     GKTVFAQGIGRGWGAADELTSPTFTLIHELHRSIDRSKLYHIDLYRLEKESEAWLLGL
                     IDLMDERASVLIEWPERARSILPPDRLWIAFETVDETQRRITFSATDPVNQSLLNSL"
     gene            651..1382
                     /locus_tag="VFF70_09170"
     CDS             651..1382
                     /locus_tag="VFF70_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZY44906.1"
                     /translation="MRHLIISDIHANLAALDAVLADAGSYDKVWHLGDLVGYGPDANE
                     VIDRLRSLPHISLAGNHDWAALGKLDLRNFNSDARETAIWTQTNLTPDNRAYLESLKP
                     IAIEEGYTLAHGSPNQPIEEYILDPLTAELNFSIFTTQVCFVGHTHWPTAYFMAQDSD
                     RLCVQRSAKWNTAVSLNDGRWIINPGSVGQPRDGNPAAAYGMLDPESGEWEYRRAKYP
                     VEVTQKRMRDLKFPNRLIDRLTHGH"
     gene            1428..2813
                     /gene="glmU"
                     /locus_tag="VFF70_09175"
     CDS             1428..2813
                     /gene="glmU"
                     /locus_tag="VFF70_09175"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZY44907.1"
                     /translation="MTAPSSNLQLPTANIQCIILAAGQGTRMKSTRPKVLHTLAGRSI
                     IDYVIDAASSISSRKPIVVIGFGAEEVQKTIGDRVQYVRQTEQKGTGHAVVQACAKID
                     PKSETVLVVYGDTPFITAQTLTALVNTRTTTSAKIALLTFQPINPANNYGRIIRDGAG
                     NITSIIEYKELTTHQRRIDEVNAGPVCYETEWLLDHLPRLQPQAGHGELYLTDLVGLA
                     AKENASITAHQGSEIEVMGINDRVELSHAEHILRERINHHWMLQGVTLIDPARTTIEA
                     SVAIGIDTVILPNTSLEGRTSIGTDCVIGPNSIVRDSQIGNHCEITASMIESAVLEDQ
                     VSVGPFSHMRKGTHLAQGVHVGNFAEVKNSQLGEGTKQGHFSYLGDATIGSNVNIGAG
                     TITANFDGEQKLTTEIGDDTFIGVDTMIIAPRKIGKRSKTGAGSVVTHDVADGELVYG
                     VPAKPKVKSKK"
     gene            2877..>3133
                     /locus_tag="VFF70_09180"
     CDS             2877..>3133
                     /locus_tag="VFF70_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZY44908.1"
                     /translation="MTASLSNELILLVVLISLNAFFAASEIAIVSVPKLHLKQLMDDG
                     NKTAELLFHLADESSRFLATTQIGMTMMGFLASATAAVSLS"
BASE COUNT          769 a          825 c          845 g          694 t
ORIGIN      
        1 ggagggaatt gagtaaactt tgattaacgg gatcggtcgc agaaaaagtt atgcgccgtt
       61 gcgtttcgtc aacggtctca aaagcaatcc acaaccgatc gggcggcaag attgatcggg
      121 cgcgctcggg ccattcgatc aacacactcg cccgctcgtc catcaagtcg atcaaaccta
      181 acagccacgc ttcggattct ttttccaatc gatagagatc gatgtgatac aacttcgatc
      241 gatcgatcga ccggtgaagt tcgtgaatca aagtaaacgt cgggctagtg agctcatcgg
      301 ctgcacccca cccccgaccg atgccctgcg cgaaaacggt cttgcccgcg ccgagttggc
      361 cggccagaca gatcacttca ccaccatgca gcaatcgacc gagccgttca ccgagctgcg
      421 cggtttgatc gggtgagcga ctggtaattt ctatggcgcc gggatctaaa gtggacatgc
      481 taaatcaaca tcgacgcttc cttcgacttc gggctgacct cttcgagttc agatctccgc
      541 tcaggatgct cgatacttcg taataaactc gcgtgattat accgccgcgc ctcaggtgcg
      601 acaagcctag ccaaagccgc tgaatcgtgt taaacttttc ggactccgct atgcgccatt
      661 taatcatctc ggatattcat gccaacctgg ccgcgttaga cgcagtgctc gccgatgccg
      721 gatcatacga caaggtctgg cacttgggcg atcttgtcgg ctacggcccc gacgctaatg
      781 aagtgattga tcggctgcgc tcgctgccgc atattagttt agccggcaat catgactggg
      841 ccgcgttggg caaactcgat ctgcgcaact tcaacagtga tgcgcgcgag actgcgatct
      901 ggacgcagac taatttgacg ccggataatc gtgcatacct ggagtcgttg aagccaatcg
      961 ccattgaaga aggttatacg ctggcgcacg gcagtccgaa tcaaccgatc gaagaatata
     1021 ttcttgaccc gttgacggcc gaattaaatt tttcgatttt cacgacgcag gtctgtttcg
     1081 tcgggcatac ccattggccc actgcctatt tcatggctca ggattcggat cgattgtgcg
     1141 tgcagcgctc ggcgaaatgg aacacagcgg tttcgctgaa cgacggccgc tggatcatca
     1201 atccaggcag cgtcggtcag ccacgcgatg gcaatccggc tgcggcgtat ggcatgctcg
     1261 acccggaatc cggcgaatgg gaatatcggc gcgccaaata tccggtcgag gtgacgcaaa
     1321 agagaatgcg cgatctcaaa ttcccgaatc gattgatcga ccgcttgacg catggacatt
     1381 gatgaatgca cactgtatag tgctgaatgc acactgctag tttttatatg actgctccat
     1441 cttccaatct ccaattacca accgctaaca tccaatgcat cattctggcc gccgggcaag
     1501 gcacgcgcat gaaatcgacg cggcctaaag tgctgcatac tctggctggc cgatcgataa
     1561 tcgattacgt gatcgacgcg gccagttcga ttagcagccg caagccgatc gtcgtgatcg
     1621 gctttggcgc agaggaagta cagaagacga tcggcgatcg agttcaatac gtgcggcaaa
     1681 ccgagcaaaa aggcaccggc cacgcggtgg tgcaagcctg tgctaagatc gatccgaagt
     1741 cggaaaccgt gttggtcgtt tatggcgaca caccttttat cactgcgcag acactgaccg
     1801 ctttagtcaa tacgcgaaca acgactagcg ccaagattgc gctgctgact ttccagccga
     1861 tcaatccagc taacaactac gggcgcatca tccgtgatgg ggcggggaat atcacttcga
     1921 tcattgaata caaagaactg acgacgcatc agcgccgcat tgatgaagtg aatgccgggc
     1981 cagtatgtta tgagaccgag tggctgttag atcatctgcc ccgcttgcag ccacaggccg
     2041 gacatggcga gttgtatttg acggatctgg ttggactggc ggcaaaagaa aatgcatcca
     2101 tcactgcgca tcaaggcagc gagattgaag ttatgggcat caacgatcgg gttgagctgt
     2161 ctcacgcgga acacatctta cgcgagcgta tcaatcacca ctggatgttg cagggtgtga
     2221 cgctgatcga tccagcccgc acgaccatcg aagcgagtgt ggccatcggg atcgacacag
     2281 tgatcttgcc caacacatcg ctcgaaggcc gaacgtcgat cgggactgac tgtgtgatcg
     2341 gcccgaactc gatcgtgcgg gacagtcaga tcggcaatca ctgcgagatc actgcctcaa
     2401 tgatcgaaag tgcagtctta gaagatcagg taagtgttgg gccgttctcg cacatgagaa
     2461 aaggcacgca cttggcacaa ggcgtgcatg tcggcaactt tgccgaagtc aagaacagcc
     2521 aactgggtga aggcacgaag caaggtcact ttagttatct gggcgacgcc accatcggat
     2581 caaacgtgaa cattggcgca gggacgatca ccgcgaactt cgatggtgaa caaaaattga
     2641 cgaccgagat cggcgacgat acttttattg gtgttgatac aatgatcatt gccccgcgaa
     2701 agatcggcaa acgatcgaaa accggagcag gctcggtggt gacgcacgat gtagcagatg
     2761 gcgagttggt gtacggtgta cccgccaaac caaaagtgaa gagtaaaaag tgaagagtga
     2821 tgatgcgctt tgtttttcac gcgtcatttg tcattgatca ttcttaatta ctccctatga
     2881 ctgctagcct gagtaacgaa ctcattctcc tcgtcgtatt gatcagcctc aatgcatttt
     2941 ttgccgcgtc ggaaattgcg atcgtcagcg tgccgaaact gcacttgaag caattgatgg
     3001 atgacggcaa caaaacagct gagctactgt ttcatttggc tgatgagtcc agtcgctttc
     3061 tggcgacgac gcagattggc atgacgatga tgggctttct ggcctcggcc accgcagccg
     3121 tgagtttgtc tga
//
LOCUS       DASRTJ010000449         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_255281,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000449 DASRTJ010000000
VERSION     DASRTJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255281"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFF70_09185"
     CDS             complement(<1..869)
                     /locus_tag="VFF70_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY44909.1"
                     /translation="MLSTALSVLKTYRNSFSPLRLPNFRLYLGGQAISLIGTWLQAAA
                     QQWVVLQLTNSEAALGIAVALSTLPILLLGLWAGVWAERWDRRKLLIGTQTAAMSLAF
                     IFAALMITGVIQVWHVYVLSFALGIVSALDFPAQQAFLGDLSGMSEVRKAVNLNAMII
                     QISRMLGPAFAGLIIARLGVSTAFWLNGLSFLAVIVTLLLVRSQQVTSPPSGKSMVHE
                     FSEGLKFIKSQPRIQDLLLFVVLVTFLGFSVLTIASAFVVKILHGNSETYGLLLSASG
                     AGALIGVLFVVPIA"
     gene            complement(947..1435)
                     /locus_tag="VFF70_09190"
     CDS             complement(947..1435)
                     /locus_tag="VFF70_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZY44910.1"
                     /translation="MPVKNNATRLLDSRKIHYEIFEYDASHFHAAAEVAQLIGVAVNQ
                     VYKTIVVLRENVVGTSRRSPLLIMVAADKEIDLKRVAASLGEKKLKVAKHDEAEALTG
                     LQVGGISALALLTKPFEPCIDRPALELSHVYVSAGQRGSNVKIGVQALIQLTNAKIVD
                     AT"
     gene            complement(1463..2068)
                     /gene="nadD"
                     /locus_tag="VFF70_09195"
     CDS             complement(1463..2068)
                     /gene="nadD"
                     /locus_tag="VFF70_09195"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZY44911.1"
                     /translation="MRLGILGGTFDPPHNGHLQMAEAAFSQLQLDKISFAPVGVQPLK
                     IDQPSTAPEQRARMVELAIANHAHFELSRIDLDRPGPHYSVDLLGIAHRQFPQSEFWF
                     IMGEDSLADLLRWRDPAGLITLARLAVQRRPSYEPNWASLEKELPDIRSRIDWIDQAE
                     INISARDIRRRVHDGLPIDNLVPQSVADYIEHHNLFSQGRV"
BASE COUNT          568 a          600 c          606 g          473 t
ORIGIN      
        1 gcgatgggca caacgaacag cacaccgatc aatgcgcccg cacccgaggc cgataacaat
       61 aaaccgtaag tttcagaatt gccatgcaat atcttcacga cgaaagcaga ggcgatggtc
      121 aagactgaga agcccaggaa agtaaccagc acgacgaaaa gcagcaaatc ttgaatgcgc
      181 ggctggctct tgatgaattt caagccttcg ctaaactcat gcaccatcga tttgccgctc
      241 ggcggactgg tcacttgctg gctgcgcacc aacaacaaag ttacaatgac cgccagaaag
      301 ctcaaaccgt tcagccagaa ggcggtcgat acgcccaatc gggcaatgat gagacccgca
      361 aatgcgggac ccagcatgcg gctgatctgg atgatcatcg cgttgaggtt aaccgctttg
      421 cgcacttcac tcatgccgga caaatcgccg agaaaagctt gttgggcggg aaaatctaaa
      481 gcgctgacga tgcccagggc gaagctcaag acatagacgt gccacacttg aatcacgccc
      541 gtgatcatca aggccgcaaa aatgaatgcc aagctcatcg cggccgtctg tgtgccgatc
      601 aacagcttgc gtcgatccca tctttcagcc caaacgcccg cccacaagcc gagcagcaga
      661 atcggcagcg tgctcaacgc gacggcgatg cccaacgctg cttccgaatt ggtcagctgc
      721 aaaaccaccc attgctgcgc ggccgcttgc agccacgtgc caataagcga gatcgcttgg
      781 ccgccgagat acaatcgaaa gttaggcagg cgcaggggtg agaaactatt gcgataagtc
      841 tttaatacac tgagcgccgt cgataacaat gcgagtctcc acgaattgga atttgattga
      901 cctcaaagat tatacctgac cgtgttgcgt aaaccttgaa gtagattcaa gttgcatcca
      961 cgatcttcgc gttggtcaat tgaatcaaag cctgcacgcc gatctttacg ttcgagccac
     1021 gctgaccagc gctgacgtaa acgtgtgata gttccagcgc ggggcgatcg atacaaggtt
     1081 caaagggttt ggtcaacaag gccaacgcgc tgataccgcc gacctgcaag ccggtcagcg
     1141 cttcggcttc gtcgtgtttg gcgactttca atttcttttc gccgagcgaa gccgccacgc
     1201 gttttagatc gatctctttg tcagcggcga ccatgatcag taggggcgag cggcggctcg
     1261 tccctacaac attctcgcgt aggacgacga tcgttttgta gacttggttg accgccacac
     1321 cgatcagctg cgcaacctca gcggccgcat ggaagtgcga ggcatcgtat tcgaatatct
     1381 cgtagtgtat cttacgtgaa tcaagtagtc gtgtggcgtt gtttttgaca ggcatgcttc
     1441 gctgttaggt gaggggcgag ttttagactc gcccctgcga aaataaattg tgatgctcga
     1501 tgtaatccgc aaccgattgt ggcacgagat tatcaatagg caatccgtca tgcacgcgcc
     1561 gacgaatgtc acgcgccgat atattgatct ccgcttgatc gatccaatcg atgcgcgatc
     1621 ggatatccgg caattctttt tcgagcgaag cccaattggg ctcgtagctc gggcgccgct
     1681 gtactgctaa tcgagcgagc gtgatcaacc cagccggatc gcgccaacgc aatagatcgg
     1741 ccagtgagtc ttcgcccatg atgaaccaaa attccgattg tggaaattgc cggtgcgcga
     1801 tccctagcaa atcgactgag taatgcggac cgggccgatc gagatcgatg cgcgagagtt
     1861 cgaagtgagc atgattggca atcgccagtt cgaccatgcg cgcgcgttgt tcaggcgcgg
     1921 tggaaggctg atcgatcttg agaggttgca cgccgacagg cgcgaacgaa attttatcaa
     1981 gctgcagttg gctgaatgca gcctcggcca tttgcaaatg accgttgtga ggtggatcga
     2041 aagtgccgcc gaggattcct aaccgcatga ggcaagttgc aagatgcaag atgcatgtta
     2101 cgcaccgcgc accacgccct ccaattggtt attgagaaac tgacgcacat ctagtaatga
     2161 agaaagaatc ttatcagccg aagacaaatc aagcgagcgc gtctgaacat tgggtacggc
     2221 aatgcatgtc atgttggcgg acttagc
//
LOCUS       DASRTJ010000450         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_66125,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000450 DASRTJ010000000
VERSION     DASRTJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66125"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFF70_09200"
     CDS             complement(<1..396)
                     /locus_tag="VFF70_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZY44912.1"
                     /translation="MKNQRTIYLDHAATTPVDPRVIEAMQPYWTDHFGNTSSIHAIGR
                     EAQQGLENARRDVAEILNCDPHEIIFTGCGTESDNLALRGVAWAARKSGPGNHIITTK
                     VEHHAVEVTAEQLHELNGFDVTFVDVDEYG"
     gene            488..1747
                     /locus_tag="VFF70_09205"
     CDS             488..1747
                     /locus_tag="VFF70_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44913.1"
                     /translation="MNPKSEAIHQKSEFKTIVVLFVIMRLTILLLYTPQGLLNAYTDT
                     NYYYRTAQLSDQGYYPFVNMWYEYPPIATYISQGVYSLTRNLPADGAIDVRYQVYSRL
                     LSGLFLIFETGVLILIHRIAAKCWETVTADRLGWIYSVLSVPLFFWNASQNSVVVFFA
                     LWSIEAFLDSHQIRSAIALGLGIATKFTPFFLLAPAIKFLLPNLRASIKYAAITIVTT
                     IAIFIPFVLLGGWQWIVASFSALGRLASYSTAWALIDGNWQPGDAGPLINRIQLDAVN
                     QLPGNPAVVPWLVTIGVLGVIYWLIFRRPIKPLGPKEYVRFSTLTLMIFLVWSKGWSP
                     QWATLVIPFLLLSFPDRRGVIITLLLTLLVFIEWPLADALQSHAWLAIAIVGRTLLFI
                     AAAFIIAREWWTPSALSRAEVAPLTQR"
     gene            2149..2580
                     /locus_tag="VFF70_09210"
     CDS             2149..2580
                     /locus_tag="VFF70_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZY44914.1"
                     /translation="MSIESNKKSARHLVEDGLNKNNMAVIDEVISAKDVEHALPPGMP
                     PTLAGFKMFISAFIAAFPDVHYTIDHEVAEGNLITHFLTGTGTMKGDFQGIKATNKKG
                     TWSEMHLGRFEGDQIVEHWAVVDQLGMLISVGAMQPPGAQH"
     gene            2698..>3397
                     /locus_tag="VFF70_09215"
     CDS             2698..>3397
                     /locus_tag="VFF70_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV restriction endonuclease"
                     /protein_id="HZY44915.1"
                     /translation="MPITFSQARASIDQLVKHFKTNLNLYRAPTYKEASARQEFIDKM
                     FVYLGWDVYNEERTAPQYRDVEVEPSQDVEGEKKAPDYVFRIGKEMKFFVEAKKPGVS
                     IKTDAKPAYQVRRYAWSAKLPLSLLTDFEELSVYDCRSRPSIDDQASVARINFFTFDQ
                     YPDRWREIWDVFSREAVRGGSFDQYIDQAKGKRGTATVDAEFLKEIEGWRETLAHNLA
                     LRNPKLSIDELNDAV"
BASE COUNT          765 a          927 c          879 g          826 t
ORIGIN      
        1 gccatattca tccacatcga cgaacgttac atcgaaaccg ttgagttcat gcaactgctc
       61 ggcggtgact tcgacggcgt gatgctcaac tttggtcgtg atgatgtgat tgccgggacc
      121 gcttttgcgc gcggcccagg ccacgccgcg cagtgcaagg ttatcgcttt cggtgccgca
      181 gccggtaaaa ataatttcat gcggatcgca gtttagaatc tcagcgacat cccggcgcgc
      241 attctccagg ccctgttgag cctcgcgccc aatggcatgg atcgatgagg tattgccgaa
      301 gtgatcggtc caataaggct gcatcgcttc gatgacgcgc ggatcgaccg gcgtggtcgc
      361 cgcatgatca aggtaaatgg ttcgctgatt cttcatcatt gctctcttgt gcaaaagatt
      421 gtaacatgtg gagggtacac ggtcaatgaa aacgaataac acttacaggt gtgagtaacg
      481 agtaatgatg aatcctaaat cagaggccat ccatcagaaa tcagagttca aaacgatcgt
      541 cgtcttgttc gttatcatgc gcctgacgat cttgctgctt tatacgccgc aaggtttgct
      601 caatgcctac accgacacca actactacta ccgcaccgcg cagctgtccg atcaaggcta
      661 ctatccgttc gtcaacatgt ggtacgaata tccgccgatc gcaacttata tttcgcaagg
      721 cgtctattca ctcacgcgca acctgccggc cgacggcgcg atcgatgtgc gttaccaagt
      781 ttattcgcgc ttattgagtg gcctcttctt gatctttgaa accggggtgc tgatcttgat
      841 ccatcggatc gcggccaaat gctgggagac cgtaaccgcc gatcgattag gctggatcta
      901 ttcagtcttg agcgtgccgc tgttcttttg gaatgccagc cagaattcgg tggtggtgtt
      961 cttcgccttg tggtcgatcg aggccttcct cgattcgcat caaattcgat cggccattgc
     1021 gctgggactg ggcatcgcca ccaagttcac gccgttcttc ttgcttgcgc ctgcgatcaa
     1081 gtttctgctg ccgaatctgc gcgcatcgat caaatacgcg gcgatcacca tcgtcacgac
     1141 gatcgcaatc ttcatcccat ttgtgttgct cggcggttgg caatggatcg tcgcttcgtt
     1201 cagcgcgtta gggcggctcg catcttattc aacggcatgg gcgttgatcg atgggaattg
     1261 gcagccgggc gacgccgggc cgctcataaa tcgtattcaa ttggatgccg tgaatcaact
     1321 gccgggcaac cccgccgttg tgccatggct tgtaacgatt ggcgtgttgg gagtcatcta
     1381 ctggctgatc ttccgtcgtc caattaagcc gctaggccct aaagaatatg tgcgcttctc
     1441 aacattgacg ttgatgatct ttttggtgtg gtctaaaggc tggagtccgc agtgggcgac
     1501 actcgtcatt ccatttctgt tgttatcatt ccctgatcgg cgcggcgtga tcatcacgct
     1561 gctcctgacg ctgctcgtgt ttatcgaatg gccgctggcc gatgcgctgc agtcgcacgc
     1621 ctggttagcg atcgccattg tgggtcgcac gctgctgttc atcgccgcgg ctttcatcat
     1681 cgcgcgtgaa tggtggactc cgtccgcgct cagtcgcgct gaagtcgctc ctctgacgca
     1741 gcgttaatcg ccggcgacga tcggcttgac ccccatactc gatcggccta tacttcccgc
     1801 atgcccatca ctttcgatca agcccaagac tcaatcaatt aactcgtcaa acaattcaag
     1861 acgaacttta acctgtattg cgcgccgaca tacaaccctg ttgcgggacg atcgatctcg
     1921 acatgtgata caatcttctt ataccgatct gaattgctcg gaagaacgat cgaggttgtg
     1981 gttggcctga gcagtcctca ggttaaatcg gaggcacatc tataaatcaa ttcgatctca
     2041 gtttgactca ccaacgatat tttgaacctc gtgcactcgg cgttcgaccg ataactccca
     2101 tcgacgccac ggcgcacccc cagccaatga atgttaagga gaatccctat gtctattgaa
     2161 tcgaataaaa agtcagcccg ccatttggtc gaagacggct tgaacaaaaa caatatggct
     2221 gtcatcgacg aagtgatatc agccaaagac gtcgaacatg ctctgccgcc cggaatgccg
     2281 ccgacgctgg cgggcttcaa aatgttcatc agtgccttca tcgccgcgtt tcctgatgtg
     2341 cattacacca ttgatcatga ggtcgccgaa ggaaatttga tcacccactt cctgacgggt
     2401 accggcacga tgaaaggcga ctttcagggc attaaagcga ctaacaaaaa gggtacatgg
     2461 agcgagatgc atctgggtcg ctttgaaggt gaccagatcg tggaacattg ggcggtcgtc
     2521 gatcagctcg gaatgttgat ctcggtcggc gcgatgcagc cgccgggcgc gcaacattag
     2581 cctggactag ttcagacgtg tatttcatat tgatgctcga aacgtaaacc tgacagatct
     2641 taagtgacct gtcaggtttt gcttgaccct cacgtcctat cattctatac ttggcacatg
     2701 cccattacct tctcgcaagc ccgagcttca atcgatcaac ttgtcaagca tttcaagacg
     2761 aacctcaact tgtatcgcgc gccgacgtac aaagaagcga gcgcgcggca agagttcatc
     2821 gacaagatgt ttgtctactt gggctgggat gtctacaacg aagaacgcac cgcgccacaa
     2881 tatcgcgatg tcgaggttga accctcacag gatgtagaag gcgaaaagaa agcgcccgat
     2941 tacgtctttc ggatcggcaa agaaatgaaa ttctttgtcg aagcgaagaa gcccggcgtg
     3001 tcgatcaaga ccgatgccaa gcccgcgtat caagtccgtc gttacgcatg gagcgcgaag
     3061 ttgccgctct cgctgttgac agactttgaa gaactatcgg tctacgattg ccgcagccgg
     3121 ccatcgatcg acgatcaagc cagcgtcgcg cggatcaact tctttacatt cgatcagtat
     3181 cccgatcgct ggcgcgagat ttgggatgtg ttttcgcgcg aagccgtgcg cggcggctcg
     3241 ttcgatcagt atatcgatca agccaagggc aagcgtggca ctgccaccgt cgatgcggaa
     3301 ttcctcaaag agatcgaagg ctggcgtgaa acgctggcgc acaatctcgc cctacgcaat
     3361 cccaaactct cgatcgatga attgaacgac gcggtgc
//
LOCUS       DASRTJ010000451         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_255517,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000451 DASRTJ010000000
VERSION     DASRTJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255517"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFF70_09220"
     CDS             complement(<1..251)
                     /locus_tag="VFF70_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44916.1"
                     /translation="MQDNDLLIVRNLKKYFPIRRGVFQRKVGDVKAVDGVSFTIKKGE
                     TLGLVGESGCGKSTTGKAILQLDRPTSGEVIFGDRDLVKL"
     gene            complement(269..1276)
                     /locus_tag="VFF70_09225"
     CDS             complement(269..1276)
                     /locus_tag="VFF70_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY44917.1"
                     /translation="MQQPQPSQQPLLQIKNLTTRFKITGAYVHAVNGISYSLNEGDSL
                     GVVGESGCGKSVSMMSVMRLIPQPPGEITAGEVLYGGRDLLKLSSEEMRHVRGKEIAM
                     VFQDPMTSLNPVMTIGRQITEMLELHMAMDRKQARARAAELLTMVGIPRAADRLDDYP
                     HQFSGGMRQRVMIAMALSCVPQVLIADEPTTALDVTIQAQIVDLVKKLRDELGMAIVW
                     ITHDLGVVARLVQNVIVMYAGFIVERSKVADLYTHPSHPYTLGLLGSLPRVDAHERAR
                     LVSIEGAPPDLVDLPTGCPFAARCKFVIDRCHHENPPLMPVGPAHEAACWVDVTTGRP
                     R"
     gene            complement(1371..1637)
                     /locus_tag="VFF70_09230"
     CDS             complement(1371..1637)
                     /locus_tag="VFF70_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44918.1"
                     /translation="MDNLIRSTLQAEIMDQAPSAAVRASMLVEAAHVRSRSAVGPSIP
                     SVANGLREQCNSPSPVAPIRLTVFSGHDVAEHWLMMIAPVHAIR"
     gene            complement(1662..2255)
                     /locus_tag="VFF70_09235"
     CDS             complement(1662..2255)
                     /locus_tag="VFF70_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY44919.1"
                     /translation="MTVTPTDAELIQRLCEGDLAALGDLYDKYRLQVFHTALSVTHDR
                     QAAEDILQECFLKVNTYARRIDTRLPLEPWLYRVTVNLAYTWVTRRSKWLTSIEDMID
                     RLVAPARTNSEYRAEMSELQAVIRQAIDSLPAAQRLTVVLYYLNSLSLKEISYILDCP
                     VGTVKSRLHYGREYLKKCLQVYHSGVPQLGPAAQSGS"
     gene            complement(2371..3138)
                     /gene="trmD"
                     /locus_tag="VFF70_09240"
     CDS             complement(2371..3138)
                     /gene="trmD"
                     /locus_tag="VFF70_09240"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZY44920.1"
                     /translation="MRFDVFTLFPEIFSSPLQESILKRAIEAGHLEVQLHNIRDYTAD
                     KHHITDDYPYGGGGGMLMKPEPVFSAVESVLGIGNEASGMRQTTAVPIILLTPQGRPF
                     NQDIAWELSKHDRLALICGRYEGFDERIRENLVTDEISIGDYVLTGGDLAALILIDAI
                     IRLRPGVLGDPEGPLDDSHTGRLLEYPQYTRPPEFRGLKVPEVLLSGDHAKIDRWRRE
                     QSLLRTVQRRPDLIAAAKLSDADRAFLENVNIESDTM"
     gene            3358..>3829
                     /locus_tag="VFF70_09245"
     CDS             3358..>3829
                     /locus_tag="VFF70_09245"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HZY44921.1"
                     /translation="MRDKPSAIMRIYITGAKGQLGRALMRRLAHHTLLGTDLPEVDLT
                     DLAAIQSSIRSFTPDVIIHAAAMTDVDGAARDPDAAYRINGLGTRNAAVSAESIGAAV
                     LAISTNEVFDGKKTEPYTEFDQTNPINPYAQSKLAAENFVRELSHRFYIVRTAWL"
BASE COUNT          913 a         1066 c         1002 g          848 t
ORIGIN      
        1 agtttcacta gatcccgatc gccaaagata acctcgccgc ttgtcggccg atcgagctgc
       61 aagatcgcct tgccggtcgt cgatttgccg cagccgcttt cgcccaccaa ccccagcgtt
      121 tcacctttct tgatcgtgaa actgacgcca tccaccgctt tgacatcgcc caccttgcgc
      181 tggaagacgc cccggcggat gggaaaatac tttttgagat tccgtacgat caacagatcg
      241 ttatcctgca ttgcattctg cgccatgatt aacggggcct cccggtggtg acatctaccc
      301 agcaagccgc ttcatgggcc gggccgaccg gcatcagcgg cggattttca tggtggcagc
      361 gatcgatcac aaatttgcag cgcgccgcaa acgggcaacc tgtcggcaga tcgaccaaat
      421 ctggcggagc gccttcgatc gaaacaagcc gagcgcgttc gtgcgcgtcg actcgcggca
      481 atgagcctaa caggcctaaa gtataaggat gactagggtg cgtgtacaaa tcggcaacct
      541 tcgaacgctc tacaataaaa cccgcataca tcacgatcac attctgcacc aggcgcgcca
      601 cgacgcccaa gtcatgggtg atccacacaa ttgccatgcc caattcatca cgcagcttct
      661 tgaccagatc gacgatctga gcctggatcg taacatccag cgcggtggtg ggttcatcgg
      721 cgataagcac ctgcggcacg cacgatagag ccatagcgat catcacgcgc tggcgcatgc
      781 cgccggaaaa ttgatggggg taatcgtcca accgatcggc ggcgcgcggg atgccaacca
      841 tcgtcaataa ttcggcggcg cgggcacggg cctgtttacg atccatcgcc atgtgcagtt
      901 ccaacatctc ggtgatctgg cggccaatgg tcataacggg attcagcgac gtcatggggt
      961 cttgaaagac catggcaatc tctttgccgc gcacgtgccg catttcttcg gacgaaagct
     1021 tcagcagatc gcgcccgcca tacaacacct cacccgccgt aatttcgccg gggggctgcg
     1081 ggattaagcg catgaccgac atcatgctga cgctcttgcc acaaccactt tcgcccacca
     1141 cgcccaaaga gtcgccttca tttaaggaat aagaaatgcc attcacggca tgcacataag
     1201 cgcctgtaat cttgaatcgc gtcgtcaggt tcttgatttg caggagcggc tgctgtgatg
     1261 gctgaggctg ttgcatgtac agtgcgtcct ccactcttga tttgtcgttc agctgctatt
     1321 aaatcagcct ctcaaggcta gacctattga gaggccggta aaaaataatc ttaacggata
     1381 gcgtgcactg gcgcaatcat catgagccag tgttcagcga catcatgccc actaaaaacc
     1441 gttaaccgta taggggcaac gggtgaaggc gaattgcact gctcgcgtaa cccgttggcc
     1501 accgacggaa tcgatgggcc gacagccgat cgggagcgga catgcgcggc ctccaccaac
     1561 attgacgccc gcacagcggc cgacggtgct tgatccatga tctccgcttg cagcgttgat
     1621 cgaattaaat tatccaccaa tcctccactc aatcttttga atcaacttcc actttgagcg
     1681 gctggcccca gctgtggcac accgctgtga tagacttgca aacacttctt gagatattca
     1741 cgcccgtaat gcaatctgga tttaacggta ccgaccgggc agtcaaggat ataagagatc
     1801 tctttcaggc ttaaactgtt caagtaatac aagaccaccg tcaagcgctg agcggcgggt
     1861 agtgaatcga tggcttgccg gatgaccgcc tggagttcag acatctcagc acgatattcc
     1921 gaattggtgc gggcgggtgc caccaatcga tcgatcatat cttcgatcga ggtcaaccat
     1981 ttcgagcggc gggtcaccca ggtataagcc agattgacgg tgactcgata cagccacggt
     2041 tccaagggca ggcgggtatc aatgcggcgg gcatacgtgt tgactttgag gaaacattct
     2101 tgcagaatgt cttctgccgc ttgacgatca tgggtaacag acagagcagt atgaaaaacc
     2161 tgtaagcggt atttgtcata caggtcgccg agggctgcga gatcaccttc gcagagtcgc
     2221 tggattagtt cagcatctgt cggggttacc gtcataccta ctataccttg acctcgaccg
     2281 aaaggttcaa ctgaggattg gaattgaacc tgtttagggt agaacaaaat ttattatact
     2341 gagatcgaag aataacgcaa gtcacacgct tcacatagta tcagactcga tattgacgtt
     2401 ctctaagaac gctcgatctg catccgatag ttttgcggcg gctatcaagt caggccgccg
     2461 ctgcacagtc cgcaacagcg attgttctcg gcgccaccga tcgattttgg cgtgatcacc
     2521 ggacaataga acttctggaa ctttcaagcc gcgaaattcc ggcgggcgag tgtattgtgg
     2581 atattccaac aagcggccgg tgtgcgaatc atcgagcggg ccttccggat cgcccagcac
     2641 acccggtcga agccgaatga tcgcatcgat caggatcagg gcagccaggt caccaccagt
     2701 cagcacgtaa tccccgatcg agatttcatc ggttacgaga ttctcgcgaa tacgctcatc
     2761 gaaaccttcg taacgcccgc agatcaatgc cagccgatcg tgcttcgaca actcccacgc
     2821 gatgtcttga ttgaacgggc ggccttgcgg agttaacaag atgatcggaa cagctgtcgt
     2881 ttgccgcata ccgcttgcct cgttgccgat tcctaacaca gactcaactg cggaaaatac
     2941 cggctccggc ttcatcagca tgccgccacc gccgccgtaa ggataatcat cagtaatatg
     3001 atgtttatcg gcggtgtaat cgcggatgtt atgcagttga acttctaaat gcccggcctc
     3061 gatcgcgcgc ttgaggatgc tttcctgcaa cgggctggag aatatttcgg ggaacaacgt
     3121 gaacacgtcg aagcgcatac agagattgta gcacggtttt gaatgtctga tctttgatct
     3181 ctgaacggca cgtcattacg agcggagcga agtaatctta ttatggtgag cacggagatt
     3241 tcttcgccgc tgcgccgctc gtaatggcgg ttaataggtt tggtataatg atctcgcttc
     3301 acgaacaatg agcgacacct gcacttcaaa gcacgcagtg cggtgcacgt gtatgccatg
     3361 cgcgataagc catctgccat catgcgaatc tacattacgg gcgctaaagg acaactcggt
     3421 cgagcgttga tgaggcgctt ggcgcaccac actttgctcg gcactgatct gcctgaagtt
     3481 gatctgacgg atctggccgc gatccaatcg agcattcgat cattcacgcc cgatgtgatc
     3541 atccacgcgg cggcgatgac cgatgtcgac ggtgcagcgc gcgatcccga tgcggcttat
     3601 cgcatcaatg gactgggcac acggaatgcg gccgtgagcg cagaatcgat cggcgcggcg
     3661 gtgttagcga tcagcacaaa tgaagtgttc gatggtaaaa agaccgagcc ttatacagaa
     3721 tttgatcaaa ctaacccgat caacccctat gcccaatcga aactcgccgc cgaaaatttt
     3781 gtgcgcgaac tttcgcatcg tttctatatc gtgcggacag cgtggctct
//
LOCUS       DASRTJ010000452         4732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_331273,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000452 DASRTJ010000000
VERSION     DASRTJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331273"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VFF70_09250"
     CDS             complement(<1..111)
                     /locus_tag="VFF70_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZY44922.1"
                     /translation="MSKQHYDRSKPHVNVGTMGQIDHGKTTLTAAITKVVG"
     gene            complement(170..242)
                     /locus_tag="VFF70_09255"
     tRNA            complement(170..242)
                     /locus_tag="VFF70_09255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Thr,seq:ggt)
     gene            complement(251..337)
                     /locus_tag="VFF70_09260"
     tRNA            complement(251..337)
                     /locus_tag="VFF70_09260"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Tyr,seq:gta)
     gene            complement(374..445)
                     /locus_tag="VFF70_09265"
     tRNA            complement(374..445)
                     /locus_tag="VFF70_09265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Thr,seq:tgt)
     gene            927..1766
                     /gene="rpsB"
                     /locus_tag="VFF70_09270"
     CDS             927..1766
                     /gene="rpsB"
                     /locus_tag="VFF70_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZY44923.1"
                     /translation="MAVVSMKALLETGVHFGHRTKRWNPKMKPFIFTERNGIHIIDLQ
                     QTLSSLDNSFNMIRDTVANGNTVLFVGTKRQAQETIAKEAVRANMPHVTYRWLGGTLT
                     NWRTIKQRIDTLHALEQRRERGEFELLTKKEALDYDRLITKLNLRLGGIKELKKLPDL
                     LFVIDVHREFTAIKEANALKIPIIAMVDTNCDPDPIDLVIPANDDAIRAIKLIVARMA
                     DAALEGAAMRKEVPQAEAVEGTAEADDKYLGEATLAKLRAGLEFEDTETAGVGVESLD
                     EQA"
     gene            1894..2490
                     /gene="tsf"
                     /locus_tag="VFF70_09275"
     CDS             1894..2490
                     /gene="tsf"
                     /locus_tag="VFF70_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZY44924.1"
                     /translation="MGISAADIKTLRAATGAGVLDCRKALEQTNGDFEKAKAWLKDKG
                     LAAAAKKTDRAASDGLVGHYIHHNSRVAVLVEVNCETDFVARTEGFKTLTQNIAKHIA
                     MAHPNYLKPEDISADEMAKLKSEFRAEAASTGKPENILDKIVESKLDNYYKEVCLLKQ
                     PYVLDDKQTIGDMVAQGIADLKENIVVRRFVRYELGGE"
     gene            2560..3273
                     /gene="pyrH"
                     /locus_tag="VFF70_09280"
     CDS             2560..3273
                     /gene="pyrH"
                     /locus_tag="VFF70_09280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZY44925.1"
                     /translation="MPDLKYKRILLKLGGEALAGQGGFGINPLMAEDLAAKIKAVKDL
                     GVEIALVLGAGNLWRGKDGLSHGMDRATADHMGMLGTVMNALALQDALERIGIGTRVQ
                     TAISMQAIAEPYIRLRAIRHMEKGRVVIIGGGTGNPYFTTDSAGALRAMEIKADILIK
                     ATKVDAIYTQDPKIHPDAQRYDELSYIDALNQRVGVIDSTAISLCMDNNMPILVLDLW
                     QAHSLERAVMGQAIGTIIR"
     gene            3521..4078
                     /gene="frr"
                     /locus_tag="VFF70_09285"
     CDS             3521..4078
                     /gene="frr"
                     /locus_tag="VFF70_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZY44926.1"
                     /translation="MAKDILKTAEEHMLKSNHALEIDLRSIRTGRASPMLVERVMVMY
                     YDTPTPLEQLATIAAPEPQLLTIKPFDVHSIKDIEKAIASSDLGFTPTDDGKMIRIAI
                     PRLSEERRNDMIKIVHKRLEEARIAVRNVRRDAHNDLRDYEKEKMLSEDELKRATDDL
                     QKLTDKHVEQAEKISQRKEEEIREV"
     gene            4116..>4732
                     /gene="uppS"
                     /locus_tag="VFF70_09290"
     CDS             4116..>4732
                     /gene="uppS"
                     /locus_tag="VFF70_09290"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZY44927.1"
                     /translation="MSQVPYHLAIIMDGNGRWAKARGLPRLAGHRAGTDNLRRILRAC
                     VKHGIKILTIYAFSTENWGRPAEEVRGLMSILEQVIERELQELHKDGVQIRHIGRMDG
                     ISPRLQKKIEHAVEMTRHNATLILNVAFNYGGRQEIIDAVRHLLKDGVDPDHVDDALF
                     SRYLYTTDLPDPDLIVRTSGEMRVSNFLIWQGAYAEYYATEALWP"
BASE COUNT         1175 a         1328 c         1262 g          967 t
ORIGIN      
        1 gcctacgacc ttggtgatgg ccgccgttaa ggtggtcttg ccgtggtcga tctgacccat
       61 ggtgccgacg ttgacgtgcg gcttgctgcg atcatagtgc tgcttcgaca tggtttcgtc
      121 tccttcaatt acttcatgct attcaatgaa atgatttctg tttttcagaa gcccacaacc
      181 agatttgaac tggtgacctt ccccttacca aggggatgct ctaccaactg agctatgtgg
      241 gcactgacac tggtggacag ggagggaatc gaacccccga acggcatcgc cgagctgatt
      301 tacagtcagc gtcctttggc cacttggata cctgtccaat tatcaatgaa cactgaccac
      361 tcatcaacgt cagagccgac ggcgagaatt gaactcgcga ccctccgctt acaaggcgga
      421 cgctctgccg ctgagctacg tcggcaacaa acaagccacg cgggctaaaa cttctagccc
      481 gctcaccgat attataccgc atattgaacg attgggtcaa gcaatcgatc gattttaatg
      541 aagcgagcga agtatacctg cgatctgggg acttgtcaaa acccgatcgg ctttcctatg
      601 cccgatcgat cttcaatctg atcgacactg ctcgtgaatg gcaaaaggat ccgatcggcc
      661 gcagaggaac aatcggcatt cagaaagagc caattttttg cggtaaccga tcgactcaat
      721 taaatcagac cgcacattcc gtttaatcga ctgtgaaata atttcattgc caacattcct
      781 gcgctgtggt atactctcgt tcgaattcgc gcaagcgaat atctcacacg ccaccgggat
      841 cgatcgaggc gtgccggagc ctacagccta ccggctctcg atcgaattga acgggacgga
      901 cgaaaaactt ttaggaggca ccacgcatgg ctgttgtttc aatgaaggca ctgctcgaaa
      961 cgggcgtgca cttcggtcac cgtaccaagc gctggaatcc aaagatgaag ccgttcatct
     1021 ttaccgagcg caacgggatc cacatcatcg atctgcaaca aaccctgtct tcgctggata
     1081 attcattcaa catgattcgc gacaccgtgg cgaacggcaa cacggtcttg tttgtcggca
     1141 ccaagcggca agcgcaggaa accatcgcca aagaagcggt gcgcgccaac atgccgcacg
     1201 tcacctatcg ctggctgggc ggcacactca cgaattggcg cacgatcaaa cagcgcatcg
     1261 acacgctcca cgcgctggag cagcggcgtg agcgcggcga gttcgaattg ctcaccaaga
     1321 aagaagcgct tgactacgat cgattgatca ccaagttgaa tctgcgcctg ggcggcatca
     1381 aagaattgaa gaagctgccc gatctgctgt tcgtgatcga tgtgcatcgc gagttcaccg
     1441 cgatcaagga agcgaacgcg ctgaagattc cgatcatcgc catggttgat accaattgcg
     1501 atccggaccc gatcgatctc gtcatcccgg cgaacgacga cgctatccgc gcgatcaagt
     1561 tgatcgtcgc ccgcatggcg gatgccgcgc tcgaaggcgc ggccatgcgc aaagaagtgc
     1621 cgcaagccga agcggttgaa ggcacagccg aagccgatga caagtatctg ggcgaagcga
     1681 ctctcgccaa actgcgcgcc ggtttggaat tcgaagatac tgagaccgca ggggttggcg
     1741 tagaatccct ggacgaacaa gcgtaacatc ctattgactt ccgaagcgca ctcgcccttc
     1801 ggaagtcttt tcaagtttga gtaaactggt tcgggcgaca gctggattgt cacccgccaa
     1861 cctcagcgca aaaatttcac ggagtgaatt caagtgggca tttcagctgc tgacatcaaa
     1921 accctgcgcg cagcaaccgg cgcgggtgtt ctcgactgcc gcaaagcttt agagcaaacc
     1981 aatggcgatt tcgaaaaggc caaagcctgg ctaaaggata aaggcttggc cgcggcggcc
     2041 aagaaaaccg atcgggcggc cagcgatgga ctggtggggc attacataca tcataacagc
     2101 cgcgtcgccg tcttagtcga agtgaattgc gagacagatt ttgtcgcgcg cacggagggc
     2161 ttcaaaacgc tgactcagaa tatcgccaag catattgcga tggcgcatcc caactacctt
     2221 aaaccagagg acatctccgc cgacgaaatg gcaaaactca agagcgagtt tcgcgccgaa
     2281 gcggcgtcaa ccggcaagcc ggaaaatatt ctggacaaga tcgtcgagag caaactggat
     2341 aactattata aggaagtctg cttgctcaag cagccgtatg tgttggacga caagcagacc
     2401 attggcgaca tggtggccca gggaatcgcc gatttgaaag aaaacattgt cgtccgccgg
     2461 ttcgttcgat acgagttggg cggcgaataa aattcaacgg tcatcgaacg ggcaacgacc
     2521 caatttcgtt gcccgttcga tgatctgatc gagatcacta tgcccgattt aaaatacaaa
     2581 cgcatcctcc tcaaactcgg cggcgaagca ttggccggtc aaggtggctt cggcatcaat
     2641 cccttgatgg ctgaagacct ggccgcaaaa atcaaagcgg tcaaagacct cggcgttgaa
     2701 atcgctctgg tattaggtgc gggcaattta tggcgcggca aagacggcct gtcgcacggt
     2761 atggatcgcg ccaccgctga tcacatgggc atgctcggca cggtgatgaa tgcgctggcg
     2821 ctgcaagatg cgttggaacg cattggcatc ggcacgcgcg ttcaaaccgc catctcgatg
     2881 caagcgatcg ccgaaccgta cattcgattg cgggcgattc ggcatatgga gaaaggccgc
     2941 gtggtgatca tcggcggcgg cacaggcaat ccgtatttca ccaccgattc ggccggcgcc
     3001 ctgcgcgcga tggagattaa agccgacatt ctgatcaaag ccaccaaggt ggatgcgatc
     3061 tacacgcaag accccaagat tcatccggat gcccagcgct atgacgagct atcgtatatc
     3121 gatgcattga atcagcgcgt aggcgtgatc gactctacgg ccatctcgtt gtgcatggac
     3181 aacaacatgc cgattctcgt gttggatttg tggcaggccc atagtttgga acgcgccgtg
     3241 atgggtcaag cgatcggcac catcatccgc tagttggcgc tcggttgcct gccagcttga
     3301 cttgcccttt tcaggccaat tcgtattata atagtgggcg cagctgggcc aatcgggttc
     3361 agctgccacc catgtttgca ccaatcgtgg taaaatcgtc ctggatctaa atcgatcacc
     3421 ccgctgccgt ttgaaatgac gtccgcttac tcatagttca acccacccaa ttttattcac
     3481 tcaatcctac ttcaaggtag agacacccgt gaccgacgac atcgctaaag atattctaaa
     3541 aaccgccgaa gagcatatgc tcaaatccaa tcacgcgctg gagattgact tgcgcagcat
     3601 tcgcaccggc cgcgccagcc cgatgttggt ggaacgtgtc atggtcatgt attacgacac
     3661 acccacaccg ctggaacaac tggcgactat cgccgcgccg gagccgcagc tgctgaccat
     3721 caagccgttc gatgtgcatt cgataaaaga cattgagaag gcgattgcat catctgattt
     3781 gggcttcacg cccaccgacg acggcaagat gatccgcatc gccattccgc gcttgagtga
     3841 agagcgtcgc aatgacatga tcaagatcgt ccacaaacgc ctggaagaag cgcgcatcgc
     3901 cgtgcgcaat gtccgacgcg atgcccacaa cgatctgcgc gattacgaaa aagaaaaaat
     3961 gctgtcggaa gacgagctca agcgcgccac cgacgatctg cagaagctca ccgataaaca
     4021 cgttgaacag gctgagaaaa tcagccagcg caaagaagag gagatccgcg aggtctaaac
     4081 ccgttcctcg caattgcatg agctccgcac ccactgtgtc tcaggtcccc tatcatctag
     4141 cgatcattat ggacggtaac ggccgctggg cgaaagctcg cggtctgccg cgcctggcgg
     4201 gacatcgcgc cggcaccgat aatctgcgcc gcatcttgcg cgcctgcgtc aagcatggca
     4261 ttaagattct gaccatttac gctttctcga ctgaaaattg gggacgtccg gccgaagaag
     4321 tgcgcggctt gatgagcatc ctggagcaag tgatcgaacg cgaacttcag gagctgcaca
     4381 aagatggcgt acaaatccgg cacatcggcc gcatggatgg catctcgccg cggttgcaaa
     4441 aaaagatcga gcacgcggtg gagatgaccc gccacaacgc taccttgatc ttgaatgtcg
     4501 cctttaatta tggcggccgc caggaaatca tcgatgcggt gcggcacctc ttgaaagacg
     4561 gcgtcgatcc ggatcacgtc gacgacgcgc tcttcagccg ctatctgtat accaccgacc
     4621 tgcccgatcc cgatctcatc gtgcgcacca gcggcgagat gcgcgtcagc aattttttga
     4681 tctggcaggg cgcgtacgcc gaatactatg cgaccgaagc cttgtggccc ga
//
LOCUS       DASRTJ010000453         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_277193,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000453 DASRTJ010000000
VERSION     DASRTJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277193"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFF70_09295"
     CDS             complement(<1..534)
                     /locus_tag="VFF70_09295"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZY44928.1"
                     /translation="MRSLFTPARRWQPAYYPFKRETFGYRLLETTERWIKGPLFGCRM
                     CGNCLLQETAFICAMECPKGARNGPCGGSTEEYCYVDETRPCIWYKIYDRSFKMGRQE
                     KLLEVLPPLDWDRVGTETWADVFKQLHTVGTRKFFGALFSRNVKQRAEVWDEVFKPVR
                     QPNWWQGDSEYHAPAYQE"
     gene            complement(666..1589)
                     /locus_tag="VFF70_09300"
     CDS             complement(666..1589)
                     /locus_tag="VFF70_09300"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZY44929.1"
                     /translation="MYIIGENIHIISEKVKAALTERNAKFFQELAVKQVEAGAQALDL
                     NLGPRKKDGEEIFPWMVDTVQAVVDVPLSFDSTNLVGIEAGLKRVTKAQPIINSTSAE
                     PERLEKVPLVAKKYNTRLVALTMGVGSIPVSADERVSIAIEKLIPRMIEIDFPMKDLI
                     IDPLVLTVSGCQQYCPHLIEAIRTLQVAWDPAPCISVGLSNVSNAVPNENRPLINRVY
                     GAMLMGVGLQTMIANPMDSEQMEVIHAIEQRNDRTPLNRLYLKIADRIGAMEEPQIED
                     FDLKDPDQMAIWKTVQILLNKVIYADSYLSQ"
     gene            complement(1651..3630)
                     /locus_tag="VFF70_09305"
     CDS             complement(1651..3630)
                     /locus_tag="VFF70_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZY44930.1"
                     /translation="MADHLVTFDVSPTPVHVPTGTLLIDAARLAGIDIGQPCGGQGRC
                     GRCAVIVNNGGVRRRSTLRLSDKDVAAGYALACQSVIEGDVEAVVLPQEKIERRLTTD
                     RVVADVTVPSGYDPDRVQTIKRVTLTLPAPSMDDQTDDWSRLQVALRQATGVDEISAS
                     LNTLRRIGSILRENDWQVAAIVDSAVRNPHSAIEDQAPAVRLIDLNPGNVPDHAPLWG
                     AAIDIGTTTVTCWLVDLISGEVKKQAAEYNRQIARGEDVISRIIYASKNGGGAELQSL
                     VVNTINALIDEVCVRASIKPTEIIKATISGNSTMIHLLLNIPAFSIRLSPFITAVNHV
                     PLLTANEIGLEINPEATIDCLPGVASYVGADITAGVLSSGLADTDKSTLFMDVGTNGE
                     IVLGSRDWLVTCACSAGPAFEGAGVIDGMRATKGAIEEVWINNDTFEPTYRVISSDKP
                     RGICGSGLISLLSELFITGVIDKGGNFNTHLGTERIRSGEHGFEYVIAFADQTEHGRD
                     IAITYVDIDNLVRAKAAIYAGFSVLADRVGVPLDSVEQILIGGSFGKYINVENAIQIG
                     LLPDLPWDRFQFLGNTSVKGAYRALLDRTDRDRIADIAARMTYIELSADNTFYEAFTS
                     ALFLPHTDLSKFPSVKEALGEKEIQTTETQRHRDF"
     gene            complement(3705..5039)
                     /gene="acsC"
                     /locus_tag="VFF70_09310"
     CDS             complement(3705..5039)
                     /gene="acsC"
                     /locus_tag="VFF70_09310"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HZY44931.1"
                     /translation="MALSGIQIYKLLPQTNCKECGFPTCLAFAMKLAAKQVELSLCPY
                     VTETSKVQLAESAAPPMRLVTVRADGHEIKVGNEVVLFRHEKTFYNKPGLFVRVRDDE
                     SIDAIKAKIKPADQFAVTYVGIDLTLDGFAIESVKNDPVVFANAIRAVREVSKRPLIL
                     ISRDPKIMSAGMLAIDGEAPMIYAADENNWEAMADLAKQHKAPLAVVADSLDRLAELT
                     EKIKAKGVEDLVIDPAARDLHTSLTLTTQIRRLALKKNFHALGYPTIAFPGESAIESV
                     AATQAITKYAGFVVLDHFLPEMIYPLLVLRQNIYTDPQKPIQVQPGLYEINNPKPEDP
                     VLVTTNFSITYFSVANEVESSGLPAWLLVADAEGMSVLTAWAAGKFDAERIAKGVKQF
                     NVADKVSKKRIVLPGHVAVLSGELEGELPGWEVRVGPREAVDLPAFMKQVLV"
     gene            complement(5096..>5831)
                     /locus_tag="VFF70_09315"
     CDS             complement(5096..>5831)
                     /locus_tag="VFF70_09315"
                     /inference="COORDINATES: protein motif:HMM:NF003376.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HZY44932.1"
                     /translation="NDPIKWAQAAEQAGADLIELTLSLTAADGSPNTPQAAVKTVKNV
                     LGATGLPLLVFGPGQAEIDNALLVPIAEATKGERLVLGICEDKNYRTIVATAMANGHL
                     VSSRTPMDVNLAKQLVILISDMGLPLDRIVMDPTTGALGYGIEYGYSVMERLRLAALQ
                     GDAMTQLPMIVTPGVEAWKTKESKVGAGVPASWGNWLERAIHWETMTSIALLESGADA
                     IVVRHPESLKRIKTAIDDLMVVKAMA"
BASE COUNT         1311 a         1662 c         1508 g         1350 t
ORIGIN      
        1 ctcttgataa gcaggcgcgt gatattcgga atcaccctgc caccaattgg gctggcgcac
       61 gggcttgaag acctcatccc acacttcggc gcgctgctta acgttccgcg aaaacaacgc
      121 cccgaaaaat tttcgtgtgc cgacggtgtg aagttgttta aacacatcag cccacgtttc
      181 cgtgccgacg cgatcccaat cgagcggcgg caagacttcc aacaattttt cctgccgccc
      241 catcttgaat gatcgatcgt aaatcttgta ccagatgcac ggccgcgtct catcgacgta
      301 gcagtactcc tcggttgagc cgccgcacgg accgttgcgc gcgcctttgg ggcattccat
      361 cgcgcagatg aaggccgtct cttgcagcaa acaattgccg cacatgcggc aaccgaacaa
      421 cgggcctttg atccagcgtt cggttgtttc caacaagcga tagccgaatg tctcgcgctt
      481 gaatggataa tacgcaggct gccaacgtcg agcgggtgtg aagagtgaac gcatggtgtc
      541 tccttctata tccgaccctc accttgcctc tccccggtac gcactgcgtg ctttaccgca
      601 tgtgtaaaag ggagaggaat gactcccttc tcctttcagg gagaaggctg gggatgaggg
      661 tcagttcatt gactcaaata actatccgcg taaatcactt tgttcaacaa aatctgcacg
      721 gtcttccaga tcgccatttg atctggatcc ttgagatcga aatcttcgat ctgcggttct
      781 tccatcgcgc cgatccgatc cgcgatcttc agatataacc gattgagcgg cgtccgatcg
      841 ttccgctgct cgatcgcatg gatcacttcc atctgctcgc tatccatggg attggcgatc
      901 atcgtctgca aaccgacgcc catcagcatg gcaccataca cgcgattgat caaagggcga
      961 ttctcattcg gcacggcatt ggacacgttc gacaaaccga ccgagatgca tggcgcagga
     1021 tcccaggcca cttgcaaagt gcggatcgct tcgatcaaat gcgggcagta ttgctggcag
     1081 ccagatacag ttaataccaa cggatcgatg atcaaatctt tcattggaaa atcgatctcg
     1141 atcatgcgcg gaatcagttt ctcgatcgcg atactgaccc gctcgtccgc ggacaccgga
     1201 atgctgccga cgcccatcgt gagcgcgacc aagcgtgtat tatatttctt ggcgaccagc
     1261 ggcactttct ccaagcgctc cggctcggcc gaggttgaat tgatgatcgg ctgggctttc
     1321 gtcacgcgct tcaagccggc ttcgatgccc actaagttgg tcgaatcaaa actgagcggc
     1381 acatcgacga cggcctgaac cgtatcgacc atccatggaa aaatttcctc gccgtctttc
     1441 ttgcgtgggc cgaggttgag atcaagcgcc tgcgcgccgg cttcgacttg tttgacggcc
     1501 aactcttgaa agaacttggc attgcgttca gtcaaagccg ctttcacttt ttcagagatg
     1561 atgtgaatgt tttcgccgat gatgtacatg gttaactcct gtatcgatca agtgagggat
     1621 aaccaccgag ccacagagtc acagaggtat ctaaaaatct ctgtgtctct gtgtctccgt
     1681 ggtttgtatt tccttttcgc ccaatgcctc tttaactgaa gggaacttgc tcaaatcagt
     1741 gtgcggcaga aacaatgccg atgtaaacgc ctcatagaat gtgttgtcgg ccgacaactc
     1801 aatataagtc atgcgcgcgg cgatgtcggc aatacgatcg cgatcggtgc gatcgagcag
     1861 cgcgcgatac gcgcccttga ccgacgtgtt gccgagaaac tggaatcgat cccacggcag
     1921 gtcgggcagc aaaccgatct ggatcgcatt ctcgacgttg atgtatttgc cgaacgatcc
     1981 gccgatcaag atttgctcga ccgagtcaag cggcacaccc actcgatcgg ccaacacgct
     2041 gaagcccgca tagatcgcgg ctttggcgcg caccaagtta tcaatatcga catacgtgat
     2101 cgcaatatcg cgtccgtgct cagtttgatc ggcgaaagcg atgacatatt caaatccatg
     2161 ctcgcctgag cgaattcgtt ccgttcccaa atgtgtgttg aagttgccac ctttgtcaat
     2221 cacgcctgtg atgaacaatt cagagagcag cgaaatcaaa ccgctgccgc aaattccgcg
     2281 cggcttatcg ctgctgatca cgcgataagt cggctcgaac gtatcgttat tgatccacac
     2341 ttcttcgatg gcgcctttgg tcgcgcgcat gccatcgatc acgcctgcgc cttcaaaggc
     2401 cggaccggcg gaacacgcgc acgtcaccag ccaatcacgc gaacctaaaa cgatttcacc
     2461 attggtgccg acatccatga acaaagtgga tttatcggtg tcggccaagc ccgatgagag
     2521 aacaccggcc gtgatgtcgg cgccgacgta actcgccaca ccgggcaaac aatcgatcgt
     2581 ggcttcgggg ttaatctcca agccgatctc attcgccgtc agcagcggga cgtgattgac
     2641 cgcagtgatg aacggcgaca aacgaatgct gaaggccgga atattcaaca gcaaatggat
     2701 catcgtgctg ttgccggaaa tagttgcctt gataatttca gttggtttga tactagctcg
     2761 cacacagact tcatcgatca gagcattgat cgtgttgacg acgagtgact gcaattcagc
     2821 accaccgccg tttttactgg catagatgat gcgcgagatc acgtcttcgc cacgcgcgat
     2881 ctgccggttg tattcggcag cttgtttctt cacctcgcca ctgatcagat caaccagcca
     2941 gcaggtgacg gtcgttgtgc cgatatcgat cgccgcaccc cacagtggtg catgatcagg
     3001 aacgttgccc gggttgaggt caatcaatct aacagcaggc gcttgatctt cgattgcgga
     3061 atgcggattg cggactgcgg agtcaacgat cgccgcgact tgccaatcat tttcacgcaa
     3121 gatcgatcca atgcggcgca aggtatttaa tgaagcagaa atttcatcta caccagttgc
     3181 ttggcgcaag gcaacttgca agcgactcca atcatcagtc tgatcgtcca tgctcggcgc
     3241 aggcaatgtc agcgtgacac gcttgatggt ctgtactcga tcgggatcat agccgctcgg
     3301 gactgtgaca tccgccacca cgcgatcggt cgtcaagcgc cgttcgatct tttcttgcgg
     3361 aagaacgacg gcttcaacat caccttcgat caccgattga cacgcgagcg cgtaaccggc
     3421 ggctacatct ttgtccgata aacgcagtgt actgcgccgc cgcacgccac cgttgttgac
     3481 aatgacggca cagcggccgc agcgaccttg tccgccgcac ggttgaccga tgtcgatgcc
     3541 cgcgagccgc gccgcatcga tcagcagtgt gccggtcggg acatggacag gtgttggaga
     3601 cacatcgaag gtgacgagat gatcggccat gttatttcga agttggacgt tggagatttg
     3661 aagattgatc caacttccaa actctaactt ccaacactcg attcctaaac taacacttgc
     3721 ttcataaagg cggggagatc aaccgcttcg cgcggaccga cgcgaacttc ccagcccggc
     3781 agttcgcctt cgagttcacc ggacaataca gcaacatgtc ctggcaatac aatccgtttc
     3841 ttgctgactt tatcggcgac attgaattgc ttcacgcctt tcgcaattct ttcggcatcg
     3901 aatttgccgg cagcccacgc cgtcagcacg ctcatgcctt cggcatccgc gacgagcaac
     3961 caggcgggca gaccggaact ttcaacttca ttcgcgacgc tgaagtaggt gatgctgaag
     4021 ttcgtcgtaa ccaacaccgg atcttcaggc ttgggattat tgatttcata caagcccggt
     4081 tggacttgaa tgggtttctg cggatcagtg taaatgtttt ggcgcaagac caatagcgga
     4141 tagatcatct ccggcaggaa atgatcgagc acgacgaagc cggcatactt cgtgatcgct
     4201 tgtgtcgcgg ccacagactc gatcgccgat tcaccgggaa aagcgatcgt cggataaccg
     4261 agtgcgtgga aatttttctt aagcgcgagc cgtcgaatct gggtggtgag cgtcagcgat
     4321 gtatgcagat cgcgcgcggc cggatcgatc accagatctt cgacaccttt ggctttgatc
     4381 ttctcggtga gttccgccaa ccgatcgagc gaatcggcga caacggctaa aggcgctttg
     4441 tgctgcttgg cgagatcggc catcgcttcc cagttgtttt catcggcggc gtaaatcatc
     4501 ggcgcttcac catcgatcgc gagcataccc gctgacatga tcttgggatc gcggctgatc
     4561 aagatcaacg gtcgtttact cacctcgcgc acagcgcgaa tcgcgttggc aaagactacc
     4621 ggatcattct tgaccgattc gatcgcaaaa ccgtcgagcg tgagatcgat gccgacgtaa
     4681 gtcacggcga attgatcggc cggtttgatc ttcgccttga tcgcatcgat cgattcatcg
     4741 tcacgcacgc gcacgaataa cccgggcttg ttataaaagg ttttctcgtg acggaacagg
     4801 acgacttcat tacccacttt gatttcatgc ccgtcggccc gaacggtcac caggcgcatc
     4861 ggcggagcgg cggattcggc cagctggact ttcgaggttt cggttacgta tgggcacaac
     4921 gaaagttcaa cttgcttcgc ggccaacttc atcgcgaaag ccaagcaagt tggaaagccg
     4981 cattctttac agttggtctg cggcagcagt ttgtaaattt gaattccaga tagagccatg
     5041 tccaactcct taacgcatac tgagtaacga gaaatgactc gttacttgtt actcgttacg
     5101 ccatcgcttt aacgaccatg agatcatcaa tcgccgtctt gatgcgtttc aaactttcag
     5161 gatgccgcac gacgattgca tccgcgccgg attccagcag tgcgatcgag gtcatcgttt
     5221 cccaatgaat tgcacgctcc agccaattgc cccacgacgc gggcacgcct gcgccgactt
     5281 tcgattcttt cgttttccac gcttcgacgc caggcgtaac aatcatcggc agttgggtca
     5341 tggcatcacc ctgcagcgcc gccagccgga gtcgttccat caccgagtag ccgtattcga
     5401 tgccgtaacc gagcgcaccg gtcgtcgggt ccatgacaat tcgatcgagc ggcaggccca
     5461 tgtcgctgat caaaatcacc agctgcttgg cgaggttcac atccatcggc gtgcgcgaac
     5521 tgacgagatg tccgttcgcc atcgccgtcg ccacaatcgt gcgatagttt ttgtcttcgc
     5581 agatgcccag caccaagcgc tcgcctttgg tcgcttcagc gatcggcaca agcagtgcat
     5641 tatcgatctc ggcttgtccc ggcccgaaga ccaacagcgg tagaccggtt gcgcctaata
     5701 catttttcac cgttttgacc gcggcctgtg gagtgttggg agaaccatcg gctgccgtca
     5761 ggctcaaagt cagttcgatc aaatccgcac cggcttgttc ggcggcctgc gcccacttga
     5821 tcggatcatt g
//
LOCUS       DASRTJ010000454         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_103582,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000454 DASRTJ010000000
VERSION     DASRTJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103582"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="fmt"
                     /locus_tag="VFF70_09320"
     CDS             complement(<1..645)
                     /gene="fmt"
                     /locus_tag="VFF70_09320"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZY44933.1"
                     /translation="MANIIFMGSPQFAVPTLEAMAADHELLAVFTQPDKPAGRGKLLT
                     AVPVKRWADAHHIPIYQPASLRKDLSAVQTIHDLKPDVIVVAAYGLILPQSVLDIPAF
                     GCLNVHASLLPRHRGAAPITSAILAGDSETGITIIQMDAGLDTGPLLAVAREPIRSDD
                     TTRSLSDRLAMLGAQLMSERLPKYLRGEIEPQPQPIEGATYSPKINKEDALIDWS"
     gene            complement(741..1961)
                     /locus_tag="VFF70_09325"
     CDS             complement(741..1961)
                     /locus_tag="VFF70_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HZY44934.1"
                     /translation="MNLTKRLIFLLFASIGLLLIPIGLAYADGITLSELTATYTFAQS
                     ITFNVQASSASPIQQATLFFQAGGAPPIKQAAAEFISANQVTLTAKLDLAHIKPPAFS
                     TISYWWEVDDRSGQHIQSPPQSIAYIDNRFQWQDLTAGSIRVHWVQGDSGFGASAASI
                     ANNALPKIQQQIGARPPAPIDIYIYSSTQQVRSAVELAGQTWLGGLAQPEFGVVMIAI
                     PDDETTLINLRRDVPHELTHLLVFVATTPNYRTVPRWLDEGLASIHEVDPNSTQAVAL
                     QNAFNANQLIPIESLCGAFPGDAASAIIAYAESRGLVQQIVNEYGSAGLQALLAAYRD
                     GATCDAGVERGLNDSLASLELKWKSSLTPSSGATSIVTGVLPWLLLIAVIVLPLMALV
                     PRKNDRGRTTNHHS"
     gene            complement(2069..2977)
                     /locus_tag="VFF70_09330"
     CDS             complement(2069..2977)
                     /locus_tag="VFF70_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZY44935.1"
                     /translation="MSTLLQILLDDILPIFIVIGFGFLFVRRTQTDMRAASRLTFFVL
                     SPCLVFTSLIESDVAGSEIVQIAAFTISITLIIGLIAFSATRIFRFDPRRTAAFLLAT
                     MFVNAGNYGLGVNRLAFGAIAESRAVIYFVTSSIMVYTIGVTIARGGQGGWRAMLTEL
                     LHLPHIYALIAALIIRSINIQVPPPILHGLELPAAAAIPMMLLLLGGQLATLSVGEYW
                     RPAFAASGISLILAPIVAIGLAALFGLSGATRQATILEASMPAAVIITIIATEYDAEP
                     KLVTATVIVSTLLSPITLTLLIAMLK"
     gene            complement(2974..>3828)
                     /locus_tag="VFF70_09335"
     CDS             complement(2974..>3828)
                     /locus_tag="VFF70_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZY44936.1"
                     /translation="IAEENFEVDFNSVYRIGLTDPDPEVRAAAIDGIWEDNDTALIAP
                     LIRFLQQDSTESVRAAAATGLGQFVLSGEYEEIPPAKIEPVLDALRSTYLKESESLEV
                     RRRALESLAYHTFDELPGFIRTAYEHSNERMRSGAVQAMGRSSDEQWSDIVVRELEST
                     NPEMRFEAAQACGGLEIPAAVKPLIELIEDVDDQVQKAAVWALGEIGGEAARRALTAV
                     LDSDQDYLHEAAEDALAELDFNSGNLAFKVLDFDDVDTVDEVDEDEEWLMDRVDEDEE
                     DDESALDN"
BASE COUNT          885 a         1016 c         1078 g          849 t
ORIGIN      
        1 gctccaatcg atcagcgcat cttctttgtt gatcttcgga ctgtaagttg cgccctcgat
       61 cggttgtggt tgcggctcga tctcgccgcg caggtattta ggcagcctct ctgacatcaa
      121 ctgtgcgccg agcatcgcca atcgatcgct aagtgatcgt gtcgtgtcgt ctgatcgaat
      181 cggttcgcgt gccacggcca gcaatggccc ggtatcgagg ccagcatcca tttgaatgat
      241 cgtgatcccg gtctctgaat caccagcgag aatcgccgaa gtaatcggtg cagcgccgcg
      301 atgccgcggt aagagtgagg catgcacatt caggcagcca aacgctggaa tatccaacac
      361 gctttgtggc aggatcaaac cgtaagccgc gacgacgatc acatcgggct tgagatcgtg
      421 gatcgtttgt actgccgaaa ggtctttgcg taaagaggcg ggttgataga tcggaatgtg
      481 atgtgcgtcc gcccagcgtt tgactggaac agctgtcagc agcttgccac gaccggcagg
      541 cttatcgggt tgagtgaaga ccgccagcaa ttcatgatcg gctgccatcg cttcgagcgt
      601 cgggacggca aattgaggtg agcccatgaa gatgatgtta gccattgttg attagaatga
      661 agccggaaaa tagaagttgg aaattgaaag cctgtcctga gcaaagccga aggattggat
      721 gtaataagtt agacgacgaa tcaactgtgg tggttggtcg ttcgtccccg gtcgtttttg
      781 cgcggcacca atgccatcaa cggcagcaca atcacggcga ttaacaacag ccacggcaga
      841 acgccagtga cgatcgatgt tgcgccgctg ctgggtgtca gcgacgattt ccatttgagt
      901 tcaagactgg cgagtgaatc attcagcccg cgctcgacgc cggcgtcgca tgttgcgccg
      961 tcgcgataag ctgccagtaa ggcttgcagt ccggcgctac catattcgtt gacgatctgc
     1021 tgcacaaggc cgcggctttc tgcatatgca attatagctg aagccgcatc gccgggaaat
     1081 gctccacaca atgattcgat cggaatgagt tgattcgcgt tgaaagcgtt ttgcagcgcc
     1141 accgcttgcg ttgaattcgg atctacttca tggattgaag ccaagccttc atccagccaa
     1201 cgcggcaccg ttcggtagtt tggcgttgtg gcgacgaaga caagcagatg tgtcaattca
     1261 tgcggcacgt cgcgccgcaa gttgatcaat gttgtctcgt catcgggaat cgcgatcatc
     1321 accacgccga actcaggctg cgccagtccg cctaaccagg tttgtccggc gagttcgacc
     1381 gcgctgcgca cttgttgcgt cgacgagtaa atataaatat cgatcggtgc aggcggccgc
     1441 gcgccaattt gctgttgaat ctttggcagc gcgttattcg cgatcgatgc cgcgctggcg
     1501 ccgaagcccg aatcaccttg aacccagtgc acgcggatcg atccggcggt caggtcttgc
     1561 cactggaagc gattgtcgat gtaggcgatc gattgcggag gcgattggat atgctgaccc
     1621 gatcgatcat ccacttccca ccaatagcta atggtgctga acgctggcgg tttgatatgc
     1681 gccagatcaa gcttggcggt caaagtgact tggttagccg aaatgaattc agcagcggct
     1741 tgtttgatcg gtggcgcgcc gccggcttga aaaaacaaag tggcttgttg aataggtgaa
     1801 gcactcgaag cttgcacgtt aaacgtgatc gattgggcga aagtgtaagt ggccgtcaac
     1861 tcgctcagtg taatcccatc ggcgtaggct aaaccgatcg ggatcagcag caagccgatc
     1921 gaggcgaaca gcaggaagat caggcgctta gtgagattca tgttcattga ataggggcga
     1981 cgcattcgcc aatagatcca tttggatcag tatgcaaatg acagcagtga tgtcgttcga
     2041 tttgaatcag tgagcgaatg cgtcgcccct acttcagcat cgcgatcaac agtgtcagcg
     2101 taatcggact caataaggtc gatacgatca ctgtcgcagt cactagtttt ggttcggcgt
     2161 catactcggt ggcgatgatc gtgatgatga ccgcggccgg catgctggct tccaagatag
     2221 tggcttgccg cgttgctcca cttaatccga ataatgcagc caggccaatg gcaacaatcg
     2281 gcgcaaggat cagactgata ccgctggccg caaaagcggg gcgccaatac tcaccaaccg
     2341 atagcgttgc cagttggccg ccgagcagca atagcatcat cgggatggcc gccgccgctg
     2401 gcaattcaag tccatgcaag atcggtggcg gcacttgaat gttgatcgat ctgatgatca
     2461 acgcggcgat caacgcataa atatgcggca gatgcagcag ctcagtgagc atggcgcgcc
     2521 agccgccctg accgccgcgc gcgatagtga cgcctatagt ataaaccatg atgctgctcg
     2581 tgacgaaata gatcacggcg cgcgattctg cgatcgcgcc gaaggccaat cggtttacac
     2641 ccaggccgta attgcccgcg ttgacgaaca tcgtggcgag cagaaacgcg gccgtgcgcc
     2701 gaggatcgaa tcgaaatatg cgtgtggcgc tgaaggcgat gagcccgatg atcaatgtaa
     2761 tcgagatcgt aaaggcagcg atctgaacga tctcgctacc ggctacatcg gactcgatga
     2821 gggatgtaaa taccaggcag gggcttaaaa caaaaaaagt caggcgcgag gcggcgcgca
     2881 tatcagtctg agtgcgccgg acaaagagaa aaccgaaacc gatcacgatg aagatcggca
     2941 gaatgtcatc gaggagaatt tggagcagtg tgctcaattg tctagcgccg attcatcgtc
     3001 ttcttcatct tcgtctactc tatccatcaa ccattcttca tcttcatcga cttcgtcgac
     3061 ggtgtcgaca tcatcaaagt ctaagacttt gaaagcgaga ttgccgctgt tgaagtcgag
     3121 ttcggccaaa gcgtcttcag ccgcttcatg caagtagtct tgatcactgt ccagcacggc
     3181 ggttaaagca cgccgcgcgg cctcgccgcc gatttcgccc agcgcccaga ccgcggcctt
     3241 ttgaacttga tcatcaacgt cctcgatcag ttcgatcaac ggtttcacgg ctgctggaat
     3301 ttccagccca ccgcaagcct gcgcggcttc aaagcgcatt tcgggattag tggattccag
     3361 ttcgcgcacg acgatgtctg accattgctc gtcactgctg cgacccatgg cttgcacggc
     3421 gccgctgcgc atgcgctcat tgctatgttc atacgcggtg cgaataaagc cgggcagctc
     3481 atcgaacgta tgataggcca gcgattccaa cgcgcggcgg cgcacttcca gtgattcaga
     3541 ctctttcaga taagttgatc gcagtgcgtc aagcaccggc tcgatcttgg ctggcgggat
     3601 ttcttcgtac tcgccgctca agacaaattg tcccaggcct gtcgccgcgg cggcgcgtac
     3661 tgattcggtt gaatcctgct gcagaaaacg gatcaacggc gcgatcaacg ccgtatcatt
     3721 atcttcccaa atgccatcga tggcggcggc tcgcacttcg ggatcaggat cggttaagcc
     3781 aatgcgataa accgaattaa aatcgacttc aaaattttct tcggcgat
//
LOCUS       DASRTJ010000455         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_103675,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000455 DASRTJ010000000
VERSION     DASRTJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103675"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1047
                     /locus_tag="VFF70_09340"
     CDS             61..1047
                     /locus_tag="VFF70_09340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY44937.1"
                     /translation="MNHSASSLIPHTSSVVSIVIVNWNGSQHLPICLNSLRRQIFKDF
                     EAILVDNNSSDVSIDLLKREYPEVKVIALRENRGFAGGSNVGLRQASGSICVLLNNDT
                     EVDPHWLQVVVDTFDRHPEAGLIASKMLRFDQRDRFHTTGDFYRLDGLPGNRGVWETD
                     RGQYNTEEYVFSACGGSAAYRRSMLDQIGLFDEDFFFSCEDIDLSWRAQLAGWQCVYA
                     PNAIVYHKLSATGGGPIASFYDGRNFIYLLAKDYPATLWQKYRNLIIRRQWKLFVEAL
                     RSWRGAAARSRMRGMLAGLIGLPKMRRKRKSVQARRTVDDEYLLSILLPPSS"
     gene            1243..1953
                     /locus_tag="VFF70_09345"
     CDS             1243..1953
                     /locus_tag="VFF70_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZY44938.1"
                     /translation="MPAYNEEQRLPDSLAQVDRFVTAQNYPIEVIIVNNNSRDNTLKI
                     AQDFAASYAYTRVLNEPRQGKGAAVRKGMLNGNGEYLFICDADLSMPIEQVNKFLPPV
                     IDRYDVAIASREIKGAQRIGEPQYRHLMGRVFNLIVRVLAIPHIQDTQCGFKVFQRNA
                     ARDVFALQTIDGWGFDVEVLFIALKRKYKLIEVPITWYYKPQTKISPIRDSMKMVFEV
                     LKVRRNGWRGQYDKQPSA"
     gene            1979..>2448
                     /locus_tag="VFF70_09350"
     CDS             1979..>2448
                     /locus_tag="VFF70_09350"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZY44939.1"
                     /translation="MKHERRLMRNGYSLIAGIDEAGRGAWAGPVVAGAVILNPEARAS
                     SRSPLHGVNDSKQLTASQREALFPIIINACIAYGIGSAQSAEIDSIGILPATRLAMQR
                     AIESLAPQPQALIIDAVKLPQVELPQSVFDFADSISLSVAAASILAKVTRDRMM"
BASE COUNT          565 a          652 c          662 g          569 t
ORIGIN      
        1 atcataccct caacaactta atcgcatcca acttccattt tccaacttct attctctaaa
       61 atgaatcact cggcttcatc cttaatccct catacttcat ccgttgtctc gattgtcatc
      121 gtcaactgga acggctcaca gcatttgccg atttgcctca actcccttcg acgacaaatc
      181 ttcaaagact tcgaagcgat ccttgtcgac aataattcta gtgatgtttc gattgacttg
      241 ctcaagcgcg agtatcctga agtgaaagtg atcgcgttga gggagaatcg cggcttcgcc
      301 ggcggatcga acgtcgggct gagacaagcg tcgggatcga tctgcgtgct gctgaacaac
      361 gatacggaag tcgatcccca ttggctgcaa gttgttgtcg atacattcga tcggcatcct
      421 gaagccggat tgatcgcctc gaagatgctg cgattcgatc aacgcgatcg atttcataca
      481 acaggcgact tctaccgcct cgacggactt cccggcaacc gtggggtgtg ggaaaccgat
      541 cggggtcagt acaatactga agagtatgtc ttcagcgcgt gcggcggcag tgcggcctat
      601 cgtcgatcga tgctggatca gatcggctta tttgatgaag acttcttctt ctcctgtgaa
      661 gatatcgatc tgtcttggcg cgcgcagttg gccggttggc agtgcgtgta cgcgccgaac
      721 gcgatcgtgt atcacaagct cagcgcgacg ggcggcggac cgattgcgtc attctacgat
      781 ggccgcaatt tcatttactt gctcgcgaaa gattatcctg cgacgttgtg gcagaaatat
      841 cgaaacttga tcattcgccg ccaatggaag ttgttcgttg aagcgctgcg atcttggcgc
      901 ggagcggccg cgcgatcgag aatgcgcggg atgctcgccg gattgatcgg gctgccgaag
      961 atgcgccgca aacgtaaatc ggtgcaagcg cggcgcaccg tggatgacga gtatctgcta
     1021 tcgatcttgc tgccgccatc gtcgtagggg cgacgcattc ccacgctgat caattttggc
     1081 ttgatcgtct gatgtatcga tgatgacgat cggtcaagac gcttggggga atgcgtcgcc
     1141 cttacacatc aattgacgat tcaatcagat cgcggtattc tagcccgcac aatattttct
     1201 tgaggccgca tcatcaacac cacgcccctg ttatcgatca tcattccagc ctataacgaa
     1261 gagcagcgac tgcctgacag tttggcgcag gtcgatcgct tcgttaccgc gcagaattat
     1321 cccatcgaag tgatcatcgt caacaataat agccgcgata acactttgaa aatcgcgcaa
     1381 gacttcgcgg caagttatgc ttatacccgc gtgttgaatg aaccgcgcca aggcaaaggc
     1441 gcagcggtgc gaaagggcat gctcaacggc aacggcgagt atttattcat ctgcgatgca
     1501 gacttatcga tgccgatcga gcaagtcaac aaattcctgc cgcccgtgat cgatcgatac
     1561 gatgtggcga tcgcgtcacg cgaaataaaa ggcgcgcagc gcatcggcga gccacagtac
     1621 cggcatttaa tgggccgcgt gttcaatcta atcgtgcgcg tgctggcgat tccgcacatt
     1681 caagacacgc agtgcggctt caaggttttt cagcgcaacg cggcgcgaga tgtgttcgcg
     1741 cttcagacca ttgacggctg gggctttgat gtggaagtgt tgttcatcgc gctgaagcgc
     1801 aaatacaaat tgatcgaagt gccgatcacc tggtattaca agccgcagac caagatcagc
     1861 cccatccgcg attcgatgaa gatggtgttt gaagtcctga aagtgcggcg caacggctgg
     1921 cgcgggcagt atgataagca accatcggcg taggggcgag cagctgctcg cccctaccat
     1981 gaaacacgaa cgccgcttga tgcgcaatgg atattcattg atcgccggca ttgatgaagc
     2041 aggccgtgga gcatgggccg ggccggtggt cgcgggtgcg gtgatattga atccagaggc
     2101 aagggcgagc agccgctcgc ccctacatgg ggttaatgat tccaagcaac ttactgcgag
     2161 ccaacgtgaa gccttgttcc caatcatcat caacgcgtgc atcgcttacg gaattggatc
     2221 ggctcaatca gctgagatcg attcgatcgg gattttaccg gccacccgac tggcgatgca
     2281 gcgcgcgatc gaatctttag cgccccaacc gcaagcattg atcatcgacg ccgtcaaact
     2341 tccgcaagtt gaactgccac aatcggtttt tgattttgcc gattcgatct cattgtcggt
     2401 ggcggctgca tcaatcctgg caaaagtcac gcgcgatcga atgatgat
//
LOCUS       DASRTJ010000456         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_201312,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000456 DASRTJ010000000
VERSION     DASRTJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201312"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="VFF70_09355"
     CDS             <1..1517
                     /locus_tag="VFF70_09355"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HZY44940.1"
                     /translation="PDHPAFDPTVRDDLARNATTSSAPHSLYAATAGGGSSQGVPLTI
                     NFDPPVYRVGMYIGDGSTLPIGGPLAALRAYNAQGVLLGQVIDRVPVPVENFFGVHAL
                     DDSIGRVELDYQHFNAEEIDDLVFDACTDDLPYHPGPATDPFSVTVRAEAHELVPMGE
                     NSQLIVTDLPGVVIVADGDNAGTPFTREIARGNAIALTAPAWALTPNGQLVVFDHWRH
                     DGVRAFADGDRTLNLTPDQAWTLTAVYVPAPGGRTYLPVVQGGASGPTPTRTSTPSST
                     PSRTPTRTPTATPTRTRVPLRTATATSTNTATPTRTPTSTPTATPTRTPTATPTSTAT
                     PIALVLTPIADAYIRSDTPTINYGSATTLYVGTQFVTMTARSLFRFDLSSIPVGATIV
                     NATFQSFATVFSPPPTTTMDIELKRIDVAWSELTVNWLTPLTYTGANNVVGVGLTPTY
                     YSWDVTSLVQTWVGGAVNNGLALVSKNEAWLVYRGFNSKESGSPPPRLLISYQP"
     gene            1674..1823
                     /locus_tag="VFF70_09360"
     CDS             1674..1823
                     /locus_tag="VFF70_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44941.1"
                     /translation="MLTTRVVHFPRGVVVRTLLVLSSILSVLALGVIVPSPTPPLSPL
                     QPAVA"
     gene            2146..3258
                     /locus_tag="VFF70_09365"
     CDS             2146..3258
                     /locus_tag="VFF70_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZY44942.1"
                     /translation="MDRREFCRYMVVVGGTVMLTPVLDACTRIEAVLTPSVSTATFGP
                     TATFVQPGPTSMITPNPTPAPTPQEKSGLAKIALVRTRDRAAGVHHAIELLGLNPIRG
                     NRVLLKPNLNSADTAPGSTHPAVLRTLVTDLYDMGAQTITLADRSGMGNTRSVMQQTG
                     VIALAQELGFTTIALDDLAEKDWAIRQSKDFHWSRGYAVPKLLLDADCVVQTCNLKTH
                     RYGGHFTMSLKNSVGLAAKTVSGGYNYMQELHNSAHQRHMIAEINTAYTPSLIVMDGV
                     EAFVNGGPDRGKKVDSEVVLAGTDRIALDAVGVAILRMFGTTPEVSSGRIFEQEQIAR
                     AVELGLGIDSPNKIDFITDDATSQAYAEKIRKTLRL"
     gene            complement(3222..3470)
                     /locus_tag="VFF70_09370"
     CDS             complement(3222..3470)
                     /locus_tag="VFF70_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44943.1"
                     /translation="MSGCRSREHDDRVCFEAIDFGQLLPSNVELEFSIQFKSVVAWIF
                     LTEPWSIIDEQHGVLWSLFSFGASASNYSLKVFRIFSA"
     gene            3707..>4260
                     /locus_tag="VFF70_09375"
     CDS             3707..>4260
                     /locus_tag="VFF70_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZY44944.1"
                     /translation="MRAQLNSGSVRLAVAAVLGLAVLLVITTFTSIAANTIAWQFAST
                     GLPASGLVRDVEFGDVNNDGKPDAVVVGVGGFGVKVYAGNGAGVWSSSGMTVGLPVGG
                     TYDRVALGDLNTDGQLDIVATGASNNGVAHWRGDGAGSWTIITTGLPITGSYVGVTLG
                     DVNQDGRLDVIAGGNGGTAAGIKV"
BASE COUNT          914 a         1256 c         1189 g          901 t
ORIGIN      
        1 tccccgatca tcctgccttc gatcccactg ttcgcgatga ccttgcgcgc aatgccacta
       61 cgtcgtcagc gccgcattcg ctgtacgcgg ccaccgcggg cggcggatcg agtcagggcg
      121 tgccgctcac gatcaatttc gatccgccgg tgtatcgcgt gggcatgtac atcggcgacg
      181 gatcgacgct gccgatcggc gggccgctcg cggcgctgcg cgcctacaac gcgcaggggg
      241 tcttgcttgg ccaggtgatc gatcgggtgc ccgtgcccgt cgagaatttc ttcggcgtac
      301 acgcgttgga cgacagcatc ggccgcgtcg aactggacta tcagcacttc aacgcggagg
      361 agatcgacga tctggtcttt gacgcctgca ccgacgatct gccttaccat cccggcccag
      421 cgaccgatcc gttcagcgtc accgtccggg ccgaagcgca tgagctggtt ccgatgggcg
      481 aaaacagcca actgattgtg actgacctgc ctggcgttgt catcgttgcc gacggcgaca
      541 atgcgggcac gccgtttacg cgagagatcg cgcgcggcaa tgccatcgcc ttgactgcgc
      601 cggcctgggc gcttacgccc aacggacaac tggtggtgtt tgatcactgg cgtcacgacg
      661 gcgtgcgcgc cttcgccgat ggcgatcgca cgctgaacct cacgcccgat caagcatgga
      721 ctttgacggc cgtgtacgtg ccggctcccg gcgggcggac gtatttgcct gtggtgcaag
      781 gcggagcgag cggaccgact ccaacgcgca catccacccc gtcatcaacg cccagccgca
      841 cacccacgcg caccccgacc gcgactccga cgcgcacgcg cgtgccgctg cgcacggcga
      901 cggccacttc gacgaacacc gcgacaccga cgcgcacgcc aacttcaacg ccaactgcga
      961 cgccaacgcg tacgccgaca gccacaccta catcaacggc tacaccgatc gccctcgtgt
     1021 tgacgccgat cgccgatgcc tacattcgat cggatacgcc gaccatcaac tacggatcag
     1081 ccacgacctt gtatgtcggc acgcagttcg tcacgatgac ggcccgatcg ctctttcgct
     1141 tcgatctcag ttcgatcccc gtcggcgcga caatcgtgaa cgccaccttc caatcatttg
     1201 cgacagtctt ctcgccgccg cccacgacga cgatggacat cgagctgaag cgcatcgacg
     1261 tcgcgtggag cgaattgacc gtcaactggc tgacgccgct tacatacacg ggcgcaaata
     1321 atgtggtcgg tgttggcctg acgcccacat actacagttg ggatgtgacg agcctggttc
     1381 aaacgtgggt tggtggcgca gtcaacaacg gcctcgcgct cgtcagcaag aacgaagcat
     1441 ggctggtgta ccgcggtttc aacagcaaag aatcgggcag tccgccgccg aggttactga
     1501 tcagctatca accgtaagag cgttgccaac ggcgaggcgt tcccgctacg tcgcgcaccg
     1561 cttatgacct gcctcatatt ggcgcgtgtc gtgatcgtga tataactgag gcattgaccc
     1621 gcgtcctgcg acggatagtc agttgccagg gcattaacca ggggaggtgt cccatgctca
     1681 cgacacgtgt tgtccatttt ccgcgcggcg ttgttgtccg cactttgctc gtcctgtctt
     1741 cgatcctcag tgtacttgcc ttgggggtaa tcgttccttc gcccacgccg cctttgtcgc
     1801 cgcttcaacc agctgtggcc taagttgagg ccgcgccagc gcttgccgct tttcatccgc
     1861 attctctaac acgcggatcg atccaaaggc aatcaccatt gcgcatattc gatttgtatc
     1921 gaattgacat gacctcgtga tgttgtagca catatgttat tgagatcgta tcattggcct
     1981 gtgttcgtgt gagatcataa tcaaatccaa tgcacctgtg gacctccccc tcgcttaggg
     2041 ccaatcgagt ataataattt tatgacgtct ctctccgcac acattcacac gaagaatcaa
     2101 tactgcaatt catcgacgac ctggggcaag tcaggagaat gaacaatgga ccgacgcgaa
     2161 ttctgtcgtt atatggtagt cgtcggggga acggtgatgc tcacacctgt gctcgatgcc
     2221 tgcacgcgga tcgaagcggt gctgacccca tccgtgtcta cagccacatt cgggccgaca
     2281 gccacattcg tccaacctgg tccaacttcg atgatcactc ctaatccaac tccggcacca
     2341 acgcctcaag aaaaatctgg actagctaag atcgcattag tcagaaccag agatcgggcc
     2401 gcaggcgtac atcatgcaat cgaactgctc ggcctcaatc ctattcgcgg caatcgagtt
     2461 ttgcttaagc ccaatctcaa cagcgccgat acagcacctg gttcaaccca tcctgccgtg
     2521 ctgcgcacgc tcgtcaccga tctgtatgac atgggcgcgc agacgatcac gttagccgat
     2581 cgaagcggta tgggcaatac acgtagcgtt atgcagcaga ccggcgtgat cgctctggct
     2641 caggaactag ggttcacaac gatcgcgctc gatgatctag cggaaaaaga ttgggcaatt
     2701 aggcaatcga aagattttca ttggtcacgt ggttatgcag taccaaaatt gctgttggat
     2761 gctgactgcg tggtacagac ctgcaatttg aagacacatc gttacggcgg acacttcacg
     2821 atgtcactga aaaattcggt cgggttggcc gccaaaacgg tgagcggcgg ctataactac
     2881 atgcaggagc tgcacaactc agcgcatcaa cgccacatga tcgccgagat caatacggct
     2941 tacacgccga gcctgatcgt catggacggt gtcgaagctt ttgtgaacgg cggaccggat
     3001 cgaggcaaga aagtcgattc cgaagtggtg ctggctggaa ccgatcgcat cgcattggac
     3061 gcagtaggtg ttgcgatttt gcgcatgttc gggacaactc cggaagtaag cagcggcaga
     3121 atctttgagc aggaacaaat tgcgcgagca gtcgaattgg ggctgggcat cgactcgccg
     3181 aataagatcg atttcatcac tgacgacgca accagtcaag cttatgctga gaagattcga
     3241 aagaccttaa ggctgtaatt cgacgcactc gcgccgaatg aaaacaaact ccacagcacg
     3301 ccatgctgtt catcgataat gctccacggt tcagtcaaga aaatccaagc gaccacactc
     3361 ttgaattgaa tgctgaactc caactcgaca ttcgatggga gcagttggcc aaagtcaatc
     3421 gcctcgaagc acactcgatc gtcgtgttcg cgcgaacggc agccactcat gactttgatc
     3481 ggcttgatcc ggccaattgc gtttgccgct ccaatagtcg atcgaaattg cgcccctgca
     3541 ggttagtata aaattttact tagtgccaaa cttttcctgt tgacgtcccg cgattatctg
     3601 tagtaaacta aaggcagtca gcatgactcg attgctgcta ttttccggct cgatcatttc
     3661 cgaagtgcag agcagtgaag catcagtata acgggaggca gacactatgc gtgctcaact
     3721 caactcagga tcagtgcgtc tggccgtagc ggccgtgctg gggcttgcgg tgctcttggt
     3781 gattacaacc ttcacctcga tcgccgccaa cacgatcgct tggcagtttg cctccaccgg
     3841 cttgcccgcc agcggcctcg tgcgcgatgt cgaattcggc gatgtgaaca acgacggtaa
     3901 acctgatgcg gtcgtcgtgg gtgtaggcgg cttcggtgtg aaagtctacg cgggcaatgg
     3961 tgctggtgta tggtcaagca gcggtatgac ggttggctta ccggtcgggg gtacgtacga
     4021 tcgggtggcg ttgggcgatc tcaacacgga cggccagctc gacatcgtgg cgacgggcgc
     4081 atcgaacaat ggcgtcgctc actggcgcgg tgatggcgca ggcagctgga cgatcatcac
     4141 gactggcctg cccattactg gatcgtatgt cggtgttacg ttgggagatg tgaatcaaga
     4201 tggccgcctc gacgtgatcg ccggcggtaa cggcggtacg gcggccggga tcaaagtttt
//
LOCUS       DASRTJ010000457         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_103801,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000457 DASRTJ010000000
VERSION     DASRTJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103801"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFF70_09380"
     CDS             complement(<1..393)
                     /locus_tag="VFF70_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HZY44945.1"
                     /translation="MNYEPVIGLEVHAELLTQSKMFCGCAVVMSDGEPPNSHVCPICT
                     GMPGMLPVINRRAVEMTIMTGLALNCTIEPFNVFARKNYFYPDLPKGYQISQYEFPLC
                     THGWLQVETSTGVKKIGIRRVHLEEDTGK"
     gene            complement(473..1657)
                     /locus_tag="VFF70_09385"
     CDS             complement(473..1657)
                     /locus_tag="VFF70_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY44946.1"
                     /translation="MRDYVSAKVNSIPPSGIRRFFDIAATKHDVISLGIGEPDFVTPE
                     PIINAGIDALRRGETHYTSNSGTEELRRGLSAYIHQRYHVEYDPETELLVTVGVSEAL
                     YLAMMAILDPGDEVIVPQPCFVSYTAEVTLAGGVPVPIATRVENNFQVTKAEIEAKIT
                     PRTKAILIGYPNNPTGAVMSRDRLIDIAKLAEQHDLIVISDEIYDRLVYAPMGDGSVQ
                     PIHTCFPSLPNMRERTILLGGFSKSHAMTGWRIGYSATSKEITAAMRKVHQYTIMSAP
                     TMAQVAALEALQHGEEYVEQMRQEYDRRRKLIVSGFNRIGLKTFEPQGAFYAFPSIKV
                     SGMTDEEFAEKLLNEERVAVVPGNAFGAGGEGFVRASYATAYEKIEEALHRMEKFMQR
                     HG"
     gene            complement(1675..2679)
                     /locus_tag="VFF70_09390"
     CDS             complement(1675..2679)
                     /locus_tag="VFF70_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZY44947.1"
                     /translation="MKVIIPLAGLGTRLRPHTYTKPKPLINVAGKPMLAHILDQLSLL
                     DVEEYIFIVGYLGEQIEQYVSREYKFKARYVMQHEMLGQAHALWLCRDYVDQAVFMVF
                     GDTFFKTDLSEITRSPTDSIAYVREIEDPRRFGVAIADQNNYVKKFVEKPSSIDDKLA
                     VIGMYYFTDGPALMKACAELMDKRIQTKGEYFLTDAINLMLTAGAKFVTRTVDVWQDT
                     GKPETVLQTNRYLLDHGYNTSEQAEYDTCVIVPPVNIHPSAKLEHSIIGPYATIGPEC
                     KITRSIIRNSIIDAGAQIEETMLDQSLIGKEAVVTSRFRRFNVGDSAVVGFSDANHDE
                     "
     gene            complement(2697..>3719)
                     /locus_tag="VFF70_09395"
     CDS             complement(2697..>3719)
                     /locus_tag="VFF70_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZY44948.1"
                     /translation="LDRVPGGSSGGSAAAVAADLCLGAFGSDTGGSVRQPASLCGVVG
                     IKPSYGRVSRYGLIAFASSLDQIGPFAKDVMDAALLLGVIAGHDPLDSTSMPNPVPDY
                     SAHFRLDLTGVKVGVPKEYFIDGLQAEVEKSVRTAIDVLRSLGAEMREVSLPHTDYGL
                     PVYYLLAPAEASANLARFDGVRYGLRIDRGEVWETYKATRGQGFGPEVKRRIMLGTYA
                     LSTGYYDAYYLKAQKIRTLIKADFDQAFKQVDVIACPTAPTTAFKIGEKVDDPLQMYL
                     SDAFTLNQNLAGICGISLPCGFDQQGLPIGLQINGPAFGEEKMLQVAYAYEQTTDWHS
                     RKPNLV"
BASE COUNT          835 a         1060 c          958 g          866 t
ORIGIN      
        1 cttgccggtg tcttcttcaa gatgcacgcg ccgaataccg atcttcttca cgcctgtcga
       61 tgtttcaacc tgcaaccagc cgtgcgtgca caatggaaat tcgtactgtg agatttggta
      121 ccctttgggc agatcggggt aaaaataatt cttgcgcgca aagacattga acggctcaat
      181 cgtgcagttc aacgcgaggc cggtcatgat cgtcatctca actgcgcggc gattgatgac
      241 cggcagcatc cccggcatgc cggtgcagat cggacacaca tgcgagttcg gcggctcacc
      301 gtccgacatg acgactgcgc agccgcaaaa cattttcgat tgtgtgagga gttcagcgtg
      361 gacttctaag ccaatgaccg gttcgtagtt catagcggat taaccacaga tatcacggat
      421 tacactgatg gattattttc gtctatacct atctgtgcga atcagtggac aattagccgt
      481 gccgctgcat aaatttttcc atgcggtgca gcgcttcttc tattttctcg tacgcggttg
      541 catatgacgc gcgcacaaaa ccctcaccgc ccgcaccgaa tgcattgccc ggcactacgg
      601 cgacccgctc ttcattcaat aatttttcgg cgaactcttc gtcagtcatg ccactgactt
      661 tgatcgacgg aaaggcgtag aacgcgcctt gcggctcgaa cgttttgagg ccgatccggt
      721 tgaagccgct cacgatcagt ttgcggcgtc gatcgtattc ttgccgcatc tgttcgacat
      781 actcttcacc atgttggagt gcttccaatg cggcgacctg cgccatcgtc ggcgcggaca
      841 tgatcgtgta ttgatgtacc ttgcgcatcg ccgccgtaat ctctttcgat gtcgccgaat
      901 agccgatgcg ccagcctgtc atcgcatgcg atttggagaa gccgcccagc aagatcgttc
      961 gctcgcgcat attcggtagc gatggaaaac aggtgtggat cggttggacc gatccatcgc
     1021 ccatcggggc atacaccaac cgatcgtaga tctcgtccga aatgacgatc agatcatgct
     1081 gctcggccag cttggcaatg tcgatcaatc gatcgcggct catcacggcg ccagtgggat
     1141 tgttcggata accgatcaag atcgctttgg tgcgcggcgt gatcttcgct tcgatctctg
     1201 ccttcgtcac ctgaaaatta ttttcgaccc gcgtcgcgat cgggaccggc acaccaccgg
     1261 ccaaagtcac ttcagccgta taagacacaa aacatggctg cggcacaatc acttcatcgc
     1321 ccggatcgag gatcgccatc atggccagat acaacgcttc cgacacgccg accgtgacta
     1381 acagttccgt ttcaggatca tattcgacat gatagcgttg atggatgtaa gcgctcaatc
     1441 cacgccgcag ttcttcggtg ccggagttgc tggtgtaatg cgtttcgccg cgccgcagcg
     1501 catcgatgcc cgcattgatg atcggctctg gcgtgacgaa gtccggctcg ccaatgccga
     1561 gcgagattac atcatgcttc gtggcggcaa tgtcgaagaa acgccgaatg ccgctggggg
     1621 gaatagaatt gactttcgct gaaacgtaat cacgcatgct ccactcctta acgcttattc
     1681 atcatgattc gcatcgctga aaccgacgac cgctgagtcg ccgacattga agcgccggaa
     1741 acggctggtc acgacggcct ctttgccaat cagcgattga tcgagcatcg tctcttcaat
     1801 ttgcgcaccg gcatcgatga tcgagttgcg gatgatcgat cgcgtgatct tgcactccgg
     1861 cccgatcgtg gcatagggcc cgatgatcga atgttccaac ttggccgatg gatggatatt
     1921 gaccggcggc acaatcacgc atgtgtcata ttctgcctgt tcactggtgt tgtatccgtg
     1981 atccagcaaa taccgattgg tctgcagcac cgtttcgggt ttgccggtgt cctgccagac
     2041 atcgacggtg cgcgtcacaa acttcgcgcc cgcagtcagc atcaaattga tggcgtccgt
     2101 taagaaatac tcgcctttgg tttggatgcg cttgtccatc aactcggcac aagctttcat
     2161 caacgccgga ccatcggtga aataatacat accaatcacc gccagcttgt cgtctatact
     2221 ggacggcttc tcaacaaatt ttttcacgta gttattttga tcggcgatcg ccacaccgaa
     2281 ccggcgcggg tcttcgatct cgcgcacata ggcaatcgag tctgtaggcg agcgagttat
     2341 ctcactgaga tcggtcttga agaatgtgtc gccaaagacc atgaacaccg cttgatcgac
     2401 atagtcgcgg cacagccaga gtgcatgcgc ctgtccgagc atctcatgct gcatgacata
     2461 gcgcgccttg aacttgtatt cacgcgacac atactgttcg atctgctcgc cgagataacc
     2521 gacgatgaag atatattctt ccacgtcaag caggctgagc tgatcgagaa tatgcgctag
     2581 catgggtttg cccgccacat tgatcaaagg tttgggcttg gtgtaagtgt gcggtctcaa
     2641 tcgagtgcca agtccagcca ggggaatgat gactttcatt ttctgctttc tagaaatcag
     2701 acgagattcg gtttgcgcga atgccaatcc gttgtttgtt catacgcata agcaacttgc
     2761 aacatttttt cttcgccgaa ggccggaccg ttgatctgca agccgatcgg taaaccttgc
     2821 tgatcgaaac cgcagggcag tgagatgccg cagatgcccg ccaaattctg attgagcgtg
     2881 aacgcatcgc tcaaatacat ttgcaacgga tcatccacct tctcgccgat cttgaaggct
     2941 gtcgtcggcg ccgtcggaca agcgatcaca tccacttgtt tgaatgcttg atcgaaatcg
     3001 gccttgatca aggtgcgaat tttttgcgct ttcaaataat aagcatcgta gtaaccggtc
     3061 gataaagcgt acgtgcccag catgatacgg cgcttcactt ccgggccaaa gccctgcccg
     3121 cgagtggctt tataagtctc ccacacttcg ccccgatcga tccgcaagcc gtaacggacg
     3181 ccgtcaaaac gcgccaagtt cgcggaagcc tccgctggtg cgagcaaata ataaacgggc
     3241 aatccataat cggtgtgcgg caaactcact tctcgcattt ctgccccgag cgatctcaaa
     3301 acatcgattg cagttcgtac cgacttttca acttcggctt gcagcccgtc aatgaaatat
     3361 tctttcggca cgccgacctt aacgcctgtc agatccaatc ggaaatgcgc cgaataatcg
     3421 ggcacgggat tcggcatcga tgttgaatca agcggatcgt gtccggcgat cacgcccaag
     3481 agcagcgccg catccatcac atctttagca aacgggccga tctgatcgag cgaggaggca
     3541 aacgcgatca gcccatagcg gctcacgcga ccatacgagg gtttgatgcc gacgacgccg
     3601 cacaacgagg ccggctgccg aaccgatccg ccggtatccg agccgaatgc gccgaggcag
     3661 agatccgcag cgaccgctgc ggcgctgccg ccgctcgatc cgccgggcac acgatcgag
//
LOCUS       DASRTJ010000458         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_103838,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000458 DASRTJ010000000
VERSION     DASRTJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103838"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFF70_09400"
     CDS             complement(<1..1363)
                     /locus_tag="VFF70_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZY44949.1"
                     /translation="MSIQPRKLLKIDLTTQQITTQSIDPRWAEDFIGGSGLAARILWD
                     SLTPSIDPLGPDNPLLFMNGPLTGLAGPSVGRFVVSSRSPATSLWAESNCGGYWGPEL
                     AFAGYDGVLITGRANQPTYIWINDDHVELRDALLLWGRTDTYETQTAIRTELHESLAR
                     IMCIGAAGERLIPYANILGDHGRVAGRTGLGAVMGSKNLKAVAVRGHHKLYIARPDQF
                     QPLRAAANRELKEDNITLGLRAGGSGAAAELFQLYGEMPMGYFTRGSFIGQENISGNV
                     MNDTILSGVTTCHACVIACGRVVAIPEGPYARSDSKGPEYETMVGFGANLLSDDLSAT
                     AHLGQKCDRYGLDTISASNTIGLAYLLYERELIKPADIGGLELNWGDPRPADFLIEMI
                     VNQHGLGAIMSRGAKALAKHFGVEELAVQVNNLEVAYHDPRAMASMMLTYATSPRGAC
                     HNQS"
     gene            complement(1435..1848)
                     /locus_tag="VFF70_09405"
     CDS             complement(1435..1848)
                     /locus_tag="VFF70_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZY44950.1"
                     /translation="MPRIFPTYEWAVAFKEEINRSLIYAEAAKTWEGAINFVIDDVLD
                     ELGHPKVLWIDLWHGQCREAKEAAATQPIEAPYTIHAPLANWKKVITKKLGPTQAMIS
                     GQLRVKGNLAHILKYVKAAQELVECATRIETEFVN"
     gene            complement(1856..2350)
                     /locus_tag="VFF70_09410"
     CDS             complement(1856..2350)
                     /locus_tag="VFF70_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44951.1"
                     /translation="MPTIDQEEFSSAIPAEARARIEKLANSLLERYKVSAPPVPIDQI
                     LKDPLLDLWDVDPTQVSSSFGHGLFRHAPRLASARLLYRAISESAAARQAGLDAPWPA
                     SRREVKYFARCVLMPEAWIRRLADADRVIDSITEKFQVPPFDVIVRLGELGLPVPDTS
                     APKA"
     gene            2494..>2840
                     /locus_tag="VFF70_09415"
     CDS             2494..>2840
                     /locus_tag="VFF70_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZY44952.1"
                     /translation="MKTIWLYVMRQAATPLHYFWEQLWQVLIGWVPTLIGVGLRGLFY
                     RLILRMDGLAAIENGVRLRFASYIRLGPRSYLDQSVYIHACPAGVRIGHDTLIMHGSI
                     LHVYNFRDLPHAS"
BASE COUNT          594 a          769 c          807 g          670 t
ORIGIN      
        1 cactctgatt gtggcacgca ccgcgcggcg aagtggcgta agtcagcatc atactcgcca
       61 tcgcgcgcgg atcgtgataa gccacttcca ggttattgac ctgcacggcg agttcttcca
      121 cgccgaagtg tttcgctaac gccttcgcgc cgcgactcat gattgcgccc aatccgtgtt
      181 gattcacgat catctcgatc aagaaatcgg ccgggcgcgg atcgccccaa ttcaattcga
      241 gtccgccgat gtctgcgggc ttgatcagtt cacgctcgta cagcaaatac gctaaaccga
      301 tcgtattgct ggcgctgatc gtgtccagcc catatcgatc gcacttctga ccgaggtggg
      361 cagttgcact caaatcatcg ctgagtagat ttgcgccgaa gccaaccatc gtttcgtatt
      421 ccggaccttt gctgtccgat cgagcgtacg gaccttcggg aatggcgacg actcggccgc
      481 aggcgatgac acacgcatgg caagtggtca cgccactgag aatggtgtca ttcatgacat
      541 tgccgctgat gttttcttgt ccgatgaaac ttccgcgcgt gaaatagccc atcggcattt
      601 cgccatacag ctggaacaat tcggcggcgg cgccgctgcc gccggcgcgc aagccgagtg
      661 tgatgttatc ttctttgagt tcacgatttg ccgcggcgcg cagcggttgg aattgatccg
      721 gtcgggcgat gtacaatttg tgatgtccgc gcacggccac ggccttcaaa ttctttgaac
      781 ccatgactgc acccaaaccc gttcgaccgg cgacgcggcc atgatcgcct aagatgttgg
      841 catacgggat caatcgttcg cctgccgctc cgatgcacat gatgcgcgcc aaagattcat
      901 gcagctcagt gcgaatcgcg gtctgcgtct cataagtgtc ggtgcgtccc cagagtaata
      961 aagcatcgcg caattcaacg tgatcgtcgt tgatccaaat ataagtgggt tgattcgctc
     1021 gaccggtaat caacacgccg tcatatccgg cgaaagccaa ctctggtccc caatagccgc
     1081 cgcaatttga ttcggcccat aaactggtcg cgggcgagcg actgctcacg acgaaacgtc
     1141 ctaccgaggg gccggccaag ccggtcaagg gtccgttcat aaacagcaga ggattatcgg
     1201 ggccgagtgg atcgatcgag ggtgtcaggc tatcccataa aatccgcgcg gccagtccgc
     1261 tgccgccgat gaagtcttca gcccagcgcg gatcgatcga ttgagttgtg atctgctgtg
     1321 tggtgagatc gatcttgaga agtttgcgtg gttgaatcga catgatgagt gattaggatt
     1381 gagtcgtgga tgctggccgg ctgatcatca atctcactca tcactgatca cgaattagtt
     1441 gacaaattca gtttcaatgc gggtcgcgca ctcgaccagt tcctgcgcgg ctttgacgta
     1501 tttcaagatg tgcgccagat tgcctttgac gcgcagttgg ccgctgatca tggcttgcgt
     1561 cgggccgagt tttttggtga taactttttt ccaattggcg agcggcgcgt gaatagtgta
     1621 tggtgcttcg atcggttgag tggcggccgc ttctttggcc tcgcggcatt ggccgtgcca
     1681 tagatcgatc cataagactt tgggatggcc gagctcgtcg agcacatcat cgatcacaaa
     1741 gttgatcgcg ccttcccagg ttttggcggc ttccgcatag attaagctgc gattgatctc
     1801 ttctttaaaa gcaacagccc attcgtaagt tggaaaaatg cgcggcatga ggctcctacg
     1861 ctttgggggc agaggtatcg ggcacgggta aacccagttc acccaagcgg acaatcacat
     1921 caaacggcgg cacttgaaat ttttcggtga tactgtcgat gacgcggtct gcatcggcca
     1981 gacggcggat ccatgcttcc ggcatcagca cgcagcgcgc aaaatatttt acttcgcggc
     2041 gtgaagccgg ccacggcgca tcgagtccgg cctggcgcgc cgcagcgctt tccgagatgg
     2101 cgcggtacag caggcgagcc gaagccaggc ggggcgcgtg ccgaaagagg ccatgaccaa
     2161 aactggaact cacctgtgtg ggatcgacat cccacagatc cagcagcgga tctttgagga
     2221 tttgatcgat cggcacgggt ggtgcgctga ctttgtagcg ttcgagcagc gagttggcta
     2281 gtttctcaat tcgcgcgcgc gcctcagccg ggatggcgga actgaattct tcttgatcga
     2341 tagtcggcat agttgattga ttggagaaaa atgaagaatc acggcgatta taccacagcc
     2401 gattatcact ctggtataat cgaaaacgct tggtaaacga accgaacgaa tctcacccct
     2461 cacggtcgcc ttgtgcgtac ttagctgagc gatatgaaaa cgatttggtt gtatgtcatg
     2521 cgtcaagccg ccacaccgct ccattatttt tgggagcagc tgtggcaagt gctgatcggc
     2581 tgggtgccta cgctgatcgg tgttggcctg cgcggcttat tctatcgatt gatcttgcgg
     2641 atggatggtc tggccgcgat cgagaacggc gtgcggctac ggtttgccag ttatatccga
     2701 ttgggtccgc gcagctatct cgatcaatcc gtctacatcc atgcgtgccc ggcgggcgtg
     2761 cgcataggcc acgatacttt gattatgcac ggctcgatct tgcatgtgta taattttcgc
     2821 gatctgccgc acgccagcat
//
LOCUS       DASRTJ010000459         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_103845,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000459 DASRTJ010000000
VERSION     DASRTJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103845"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFF70_09420"
     CDS             complement(<1..206)
                     /locus_tag="VFF70_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY44953.1"
                     /translation="MALIQFTRNHNDSSTDQGFQFEFLCDRCGNGYQTEFQASATGLA
                     TSALDAASNLFGGLFSNVANAGHSV"
     gene            complement(377..1138)
                     /locus_tag="VFF70_09425"
     CDS             complement(377..1138)
                     /locus_tag="VFF70_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZY44954.1"
                     /translation="MDQIELNVAPREVTGKQVSVLRRAGLVPVVMYGRHDKPLVLQAK
                     ARELMKVLSKAGSSRLISIKVDGESDTHMALVREIQREPIRGDLLHTDFYAVSMTEEI
                     TVEVSIHFTGASPAAVRNEGVLTYGATTIEIECLPGDLIDSISVDLSSLAQVGDAIHV
                     RDLTIPGTIKVLDDADDLVVRVTYLAAEEVEPVPGAAVTEAVVEPEVIKKGKIEEEEI
                     EDGAAAKPGGAAKPAAAKAEAKPAGKAEAKPAAKK"
     gene            complement(1181..1678)
                     /locus_tag="VFF70_09430"
     CDS             complement(1181..1678)
                     /locus_tag="VFF70_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44955.1"
                     /translation="MSEETVEFRGQSIGDVNAHAVHMSQSTARELEADRVELHQSAVQ
                     LIDADSIELRQSAAQMIDADTLRMQQSLTLGARTETLQAEQSVLGMVRTHEATLADSS
                     VLALAGDHIQAANVNTVFLIGRNIEGDIKTMMDTRGALMFGLAAGGVLGFFSLISALA
                     RRFRR"
     gene            complement(1968..>2299)
                     /locus_tag="VFF70_09435"
     CDS             complement(1968..>2299)
                     /locus_tag="VFF70_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44956.1"
                     /translation="INRACVGCHVMQLVTVRFANHFHVKLPQAWNLIQSGVQLIVAAD
                     TPQVLTDPRSKPELLNTSITCLSCHQAHRSNLDLTDYLDQDAVVLQACAACHRETGKG
                     PVGIAPP"
BASE COUNT          481 a          703 c          609 g          506 t
ORIGIN      
        1 accgagtggc cagcattcgc cacattgcta aacaaaccgc cgaacaaatt gctggccgca
       61 tctaaagccg aggtcgccag tccggtcgcc gaggcctgaa attcggtctg ataaccgttg
      121 ccgcatcgat cgcataagaa ctcaaactga aaaccttgat cggtgctgct gtcgttgtga
      181 ttgcgggtga attgaatgag cgccatgctg ttctcctgtg atgaaaatga tcaaaacatt
      241 ttcggctgaa ggcgtgattt ctggcaccga gtatagtcca aatcgatcag gcgtcaagcg
      301 catctcatcc cgccaaacaa aaaaatcgcc gggcggaatt ttccacacgg cgatttgatt
      361 tgctggcaac aaacagttac ttctttgcag caggcttggc ttcggctttg cccgcgggct
      421 tcgcttcggc cttggctgcc gccggtttgg ctgcgccacc cggcttggcg gctgcgccgt
      481 cctcgatctc ttcttcctcg atcttgccct tcttgatcac ttccggttcg acgaccgcct
      541 cggtcacggc cgcacccggt accggctcca cttcttcggc ggcgagataa gttacccgca
      601 cgaccaaatc atccgcatca tccaagacct tgatcgtgcc ggggatggtc agatcgcgga
      661 catgaattgc atcgccgact tgcgccaggc tgctgagatc gacgctgatc gaatcgatca
      721 aatcgccggg caagcattca atctcgatcg ttgtcgcgcc gtaggtcaac acgccttcgt
      781 tgcgcaccgc cgccggtgaa gcgcccgtaa agtgaatcga cacttcgacg gtgatctctt
      841 cggtcatgga gaccgcgtag aaatcggtgt gcagcaaatc gccacggatg ggttcgcgct
      901 gaatctcgcg caccaacgcc atatgcgtat cactttcgcc gtcaaccttg atcgatatca
      961 ggcggctgct gcccgctttg gacagcacct tcatcagctc gcgagccttg gcctgcagaa
     1021 ccagcggctt gtcatgccgg ccatacatca ccaccggcac cagccccgcc cggcgcaaga
     1081 cactgacctg cttgccagtc acttcgcgcg gtgcaacatt gagttcaatt tgatccatcg
     1141 ttcctcagct cctcgacgtg cagcgcacgt gttcaatctt ttagcggcga aaacgccgtg
     1201 ccaatgcaga gatcaatgaa aagaaaccta ataccccgcc ggctgctaaa ccgaacatca
     1261 acgcgccgcg cgtgtccatc atcgttttaa tatcgccttc gatgttgcgg ccgatcaaga
     1321 agaccgtgtt gacattggct gcttgaatat gatcgccggc cagcgccagc acactgctgt
     1381 cggccagcgt ggcctcatgg gtgcgcacca tgcccagcac cgattgctcg gcttgcagcg
     1441 tctcggtgcg tgcgcccaac gtcaggcttt gctgcatccg cagtgtatcg gcgtcgatca
     1501 tctgcgcggc tgattggcgc agctcgatcg aatctgcatc gatcagctgt acggccgatt
     1561 ggtgcagctc tacccgatcg gcttccagct cccgcgcggt cgattgtgac atatgcacag
     1621 cgtgcgcgtt cacatcccca atcgattgac ccctgaactc aacggtttct tcggacatag
     1681 taagtctcaa tctttccagc gttcaccgaa ttcaatccag aacgctctca ggtcaaatag
     1741 gcaaacaaaa aggcgcggcc ccaatcgcgg cgacccgccc cacggcccca ctcacactgg
     1801 cggcattgta cctgttttag cctgattcgt caaatgcacg cggcacacga aggttatcag
     1861 gccatcagcc gaagcggctc agtgtgaagc ctccctgtgg gacatgtcgc ttgaggtcgg
     1921 gcgtgggagc ccgacccgct gatatgtatt gcatgtacat cggtctttta cggcggcgca
     1981 atcccgaccg ggcctttgcc cgtttcgcga tggcaggcgg cgcaggcttg taaaacgacg
     2041 gcatcttgat cgagataatc cgtcaaatcc aaattcgatc gatgcgcttg atggcagctc
     2101 aagcaggtga tggacgtatt cagcagttcc ggtttcgatc gaggatcagt caagacctgc
     2161 ggcgtgtctg cggcgacgat cagctgcacg ccgctttgaa tcaagttcca ggcctgcggc
     2221 agcttgacgt gaaaatgatt ggcgaaccta accgtgacga gctgcatgac gtgacagccc
     2281 acgcatgcgc gattgatca
//
LOCUS       DASRTJ010000460         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_66809,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000460 DASRTJ010000000
VERSION     DASRTJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66809"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1654)
                     /locus_tag="VFF70_09440"
     CDS             complement(164..1654)
                     /locus_tag="VFF70_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZY44957.1"
                     /translation="MLSILDNPLISTIRSITTERNIHAWLVGGAVRDWLLGRSIHDWD
                     IAVEREAIPLARALADRLQADVYVLDPDRDTARVIMADAVIDIARLRVASIEGDLEAR
                     DFTINALAIDLAEPDRLIDLFNGQRDLNDGLIRAISEEALTTDPIRLLRGVRLSASLL
                     FGLDPITAGWIKSHSALIQSASAERVRDELLKTLIEPGTADNLILLDAFDLLHTVLPE
                     VVAMHGVEQSLPHHWDVFEHTRRVVDSLELILTRVLGWPQADESALMLPVIPAFVWDG
                     LIYTLSPYTDSLRAHLQIDANDLERSRLSLLKWAALLHDIGKPSARTVDPDGRSRFFT
                     HEDVGAELAADRMRELRFNKDAIDRVSGIIRSHMRPHHLAKSAVTRRAIYRFFHACGD
                     YGVDVLLLSIADRLATHGPDLERDYWVTRLGMIDQMLSAYFEQGAPIIAPPQLVTGDD
                     VMSALNLKPGKHIGAILEAIREAQADGEVQTKEEALQVARRMLEEK"
     gene            complement(1711..1899)
                     /locus_tag="VFF70_09445"
     CDS             complement(1711..1899)
                     /locus_tag="VFF70_09445"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HZY44958.1"
                     /translation="MKAPTQPGHPIDLAPYAGRWIAVVRDHVAGVGRNAQEAAALAKT
                     TRPKEEPMVLFVPENFGG"
     gene            2126..>2475
                     /locus_tag="VFF70_09450"
     CDS             2126..>2475
                     /locus_tag="VFF70_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY44959.1"
                     /translation="MKLAARMGRLGTETAFEVLARARALEAQGRDVVHLEIGEPDFGT
                     PQHIIDAATHALNNHATHYTPSAGIPELRKTIADYVSRTRNLDVTPDMVVVVPGGKPI
                     IFHGLMALIERVDEV"
BASE COUNT          564 a          692 c          690 g          529 t
ORIGIN      
        1 ctgcttcaag ggtttatgca tgcggcgcga ctttggagag actcgcgcta agcgcgatca
       61 tgctaggcgg agattcaaat gatcacaggc ggaatcgatc gaggcgggtg gcggcaggtg
      121 ttcgcatcga catcgagcac aagaatagat cacgcagcac gcatcacttt tcttccagca
      181 tgcgtctggc aacttgtagt gcttcctctt tcgtctgcac ttcgccatca gcctgcgcct
      241 cacggatcgc ttccaagatc gcgccgatgt gtttgccagg tttgagattt aacgccgaca
      301 tgacatcatc gcccgtgacg agctgcggcg gcgcaatgat gggcgcacct tgctcaaagt
      361 acgccgacag catctgatcg atcataccca ggcgcgtcac ccagtaatcg cgttcgagat
      421 cggggccatg cgtcgccaaa cgatcggcga tggacaacaa caggacatcg acgccgtaat
      481 cgccacaggc gtgaaagaag cgatagatcg cgcggcgggt gactgcgctc tttgccaaat
      541 gatgcggccg catgtgcgat cgaatgatgc cgctgacgcg atcgatcgcg tccttgttga
      601 aacgcagttc gcgcattcga tcggcggcca gttccgcccc gacatcttcg tgggtgaaga
      661 agcggctgcg cccatccgga tcgactgtgc gtgctgacgg cttgccgatg tcgtgcagca
      721 gcgccgccca cttgagcaga ctcaacctcg atcgttccag atcgttcgca tcgatctgca
      781 aatgcgctcg cagactatcg gtgtagggtg acaatgtgta aatcaagcca tcccatacaa
      841 acgctggaat gaccggcagc atcaaggcgg attcatcggc ttgcggccag cccaagacgc
      901 gcgtcaagat caattcaagt gaatcgacga cacggcgcgt atgctcgaag acatcccaat
      961 gatgcggcag cgattgttcc acgccatgca tggcaaccac ttcaggcaga acagtgtgaa
     1021 gcagatcgaa cgcatcgagc agaatcaagt tatcggccgt acccggttcg attaaagttt
     1081 tcaagagttc atcacgcacg cgttcggctg atgctgattg aatcagagcg ctatgggatt
     1141 ttatccagcc agccgttatt ggatcaaggc caaataacaa cgaggccgac agccgcacgc
     1201 cgcgcaacaa acggatcgga tcggtagtca atgcttcttc gctaatcgcg cggatgaggc
     1261 cgtcgttcag atcgcgttgg ccgttgaaga gatcgatcag ccgatcgggt tcagcaagat
     1321 cgatcgccag ggcattgatc gtaaaatcgc gtgcttcaag atcgccttcg attgaagcga
     1381 cacgcaaccg ggcaatatcg atcaccgcat ccgccatgat gacccgcgcc gtatctcgat
     1441 cgggatcgag cacatagaca tcggcctgca aacgatcggc caacgcgcgt gcgagcggaa
     1501 tcgcctcgcg ctcgacggca atatcccaat cgtggatcga tcgaccgagc agccagtccc
     1561 gcactgcgcc gccgaccagc cacgcgtgaa tgttgcgctc agtggtgatc gagcgaatgg
     1621 tgctgatgag cggattatcc agtatcgaga gcaaaggtca attcctaatc tgacaagatg
     1681 atcttttgac aaggtcagcg tgtcatcttg tcatccccca aagttttccg gcacgaacaa
     1741 aaccatcggt tcctctttgg gcctcgtcgt cttcgctaaa gcagccgctt cttgcgcgtt
     1801 gcgccctacg ccggccacat gatcgcgaac cacggcaatc cagcgtccgg catacggcgc
     1861 aagatcgatc ggatgaccgg gttgggttgg cgctttcatg ccggaatgat aggtgaaatc
     1921 aagcgaatgg tcaatgtctg atgatcagtt aatcggagtt cagccttctg ggatctcgtg
     1981 tcgcgctcta tcaagtaccc atcacttttc tttatcattt gtcattattc atttgtcata
     2041 cttcattgtt cattgttaat tgagaattga tcatggtaca atgtcgctca gagtccaatc
     2101 ttattaggcg ggggagagca agcgcatgaa attagcagca cggatgggcc gattgggcac
     2161 cgagactgcc ttcgaggtgt tggcccgcgc cagagcactg gaagcgcagg gccgtgatgt
     2221 ggttcatctg gagattggtg agccggattt tggcacgccg cagcacatca tcgatgcagc
     2281 cacgcacgcc ctgaacaatc acgccacgca ttacacgcct tcggccggca taccggaact
     2341 gcgcaaaaca atcgcggact atgtcagccg cacgcgcaac ctcgatgtca cgccggatat
     2401 ggtcgtcgtg gtgccgggcg gcaagccgat catctttcat ggcttgatgg cgctgatcga
     2461 gcgtgtggat gaagt
//
LOCUS       DASRTJ010000461         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_352985,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000461 DASRTJ010000000
VERSION     DASRTJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352985"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFF70_09455"
     CDS             complement(<1..311)
                     /locus_tag="VFF70_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY44960.1"
                     /translation="MIELTFIGTGAAIPPADRGNAAFTVRTDNFLFLADAGPTIFNGL
                     QRAGIEPTAIDALYLSHGHADHILGMPQIALVSKFIPRTPPLKIFSTADVRAAVTTIQ
                     R"
     gene            complement(523..1932)
                     /locus_tag="VFF70_09460"
     CDS             complement(523..1932)
                     /locus_tag="VFF70_09460"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZY44961.1"
                     /translation="MSPLNTLLNSVHHTLELKDNRVSVKDERHLLEHTDDLINAAVFG
                     DAETKAVARWLIWETAQALGIRPASIHDLYLARGRGEVNTTFTVPAINIRMLAYDTAR
                     AALRAARKLNISAMIFEIARSEIGYTDQRPGEYVAVLLAAAIKEGHRGPLFIQGDHFQ
                     INAKKYAADPIAELKAVKDLIAEAVPAGFLNIDIDTSTLVDLSKPTTAEQQRSNFELC
                     AQLTQFVRSKEPQGVTISIGGEIGEVGGKNSNEEEMRAFMEGYNQSLSGSIGMSKISI
                     QTGTSHGGVVMADGKLADIAIDFDVLKRLSQLAREAYHMGGAVQHGSSTLPQNAFSKF
                     LDCAAVEVHLATAFQTLVFEHKAIPNDLRQRMIEWVKTNAADERKATDSDEQFIYKSR
                     KKAIGPFKHDLWSLPVEARNAICADLEDRFAFLFKQLNVVDTQSIVEKFVHPIEWHQP
                     LPTGALQAIEQESIEGLAD"
     gene            2165..>3918
                     /locus_tag="VFF70_09465"
     CDS             2165..>3918
                     /locus_tag="VFF70_09465"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZY44962.1"
                     /translation="MRGFRIVILLSVLISIGAVVPIVQAASTCSLTWDRYDADIQVNT
                     DGTMTVTEIETIHFTGSCHKGFRVIPQSHGTTDNIVVWEGDRRYSSSNAGSEYSYSTF
                     HDDNGDINLTWYFPYTSNSTHVYNIQYTVQTGLLYYPKDDYDRIQWKAIPADHAYPIL
                     NSNVTLHLPASAPILNDNNGPRIGVADGNLAVASAGSDRSTARFEAISPLSPGNGIEI
                     RADFQHGAVAGTAPAWQTQYDFQDKYGPIISLAGCVLGLLLMLGGVLWVYLRWYTRGR
                     DPLTGLVTEYVTEPPSDLPAGMVGTLVDEKADMQDIIATLIDLARRGSITMQEEQTAG
                     FLGIGSSRDFIFKRIANSNDSQLRDYERTLLNDVIPSGERKLSDLKNSFYTSIPALKK
                     GLYAEVVKEGLFPASPDSVRSRWAGVGCGIIFLAILGGIVVGVLFANTAFGVVFPFIG
                     LGFFGLVTMSASAAMPVKTRKGSEAKAAWLAFKRYLENIKQYTKVEGATAQFDKYLPY
                     AIAFGIDRSWINTFAQVNTPVPIWYYPYGYGGPIGSGSSMSGGGVGNIGTPSVQGMSN
                     SLAGSLQGMSTGLTSMLN"
BASE COUNT          898 a          975 c         1075 g          970 t
ORIGIN      
        1 aagcgctgaa tcgttgtgac agctgcacgg acgtccgccg tcgaaaaaat ttttagcggc
       61 ggagttcgtg ggataaattt ggatacgagc gcgatctgcg gcatacctaa gatgtgatcg
      121 gcatgaccgt gcgacagata gagcgcatcg atggccgtgg gctcaatgcc cgcgcgttgc
      181 agcccgttga aaatcgtcgg accggcatcg gcgagaaata aaaaattgtc agtgcgaacg
      241 gtgaaagcgg cgtttcctcg gtcggccggc ggaattgccg cgccagtgcc aataaacgtt
      301 agctcaatca catctaaacc tttgaaggaa gattgtattg aatgaaatgc ttgccaatga
      361 caggctcgga ataatagctc ggtcagaagc aattatcaaa tcatgtttcg gagtgtcata
      421 cctgcgaaag caaggatcca gtcggttgct ctggattccc gcctttgcgg aaattacact
      481 aacatcacaa gatctctgat tttgggaaga aggaggtcaa cattaatccg ccaacccctc
      541 gatcgattct tgttcgatcg cttgcaatgc cccagtcggc agcggttgat gccattcgat
      601 tggatgaacg aatttctcca cgatcgattg tgtgtcgacc acattcagtt gtttgaacaa
      661 gaaggcaaat cgatcttcca ggtctgcaca gatggcgttg cgcgcttcaa cgggtagact
      721 ccataggtcg tgcttgaacg gaccgatcgc tttcttgcgc gacttatata taaattgctc
      781 gtcggaatcg gtcgctttgc gttcatcggc tgcgttcgtc ttgacccact cgatcatgcg
      841 ctggcgcagg tcatttggta tcgccttgtg ctcaaacacc agcgtttgaa aagcggtggc
      901 gagatgaact tccactgccg cgcaatctaa aaatttgctg aacgcatttt ggggcaaggt
      961 gctggagcca tgttggacgg cgccgcccat gtgatacgcc tcgcgcgcta attggctcaa
     1021 gcgcttcaag acatcaaaat cgattgcaat atcggccagc ttaccgtccg ccataacgac
     1081 gccgccgtgc gatgtgccgg tctggatgct gatcttgctc atgccgatcg atccgctgag
     1141 cgattgattg taaccctcca tgaacgcgcg catttcttct tcgtttgaat tcttgccgcc
     1201 aacttcgcca atctcacctc cgatcgaaat ggtgacgccc tgcggctctt tcgatcgaac
     1261 aaattgagtg agctgcgcgc acaattcaaa attactgcgc tgttgttcag cggtcgtcgg
     1321 cttcgagaga tcgacgaggg tggaagtgtc gatatcgata ttcaggaatc cggccggcac
     1381 tgcttcggcg atgagatctt tgacagcctt tagttctgca atgggatccg ctgcatactt
     1441 cttggcattg atttgaaagt gatcgccttg aatgaacaac gggccgcgat ggccttcttt
     1501 gatcgcagcg gccagcagca cggccacgta ttcgccgggg cgttgatcgg tgtagccgat
     1561 ttcgctgcgg gcgatctcga agatcatcgc gctgatattg agtttccgag cggcgcgcag
     1621 ggcagcgcgc gcagtatcgt aggctagcat ccggatgttt atcgcaggca ctgtaaaggt
     1681 ggtgttgact tcaccccgac cgcgagccaa atacagatca tggatcgatg cagggcgaat
     1741 gcccaaggct tgtgccgttt cccagatcaa ccagcgggcc acggctttag tctcggcgtc
     1801 gccgaatact gcggcgttga tcaggtcgtc cgtatgttca agcaaatggc gctcgtcttt
     1861 tacgctgacg cgattgtctt ttagttcgag ggtgtgatgc acggaattga gtaacgtgtt
     1921 gagtggtgac atggactgtg gctccggttc aaaatgatca agagggttca tttctaagca
     1981 ttgaatctgt attgaagtgt aatgtcggtt aaggagtttg tcaaacgaaa gctcgcacct
     2041 tttctgagtg attaacgtca tggtgcgttt acacatttgg cggctttatc agtataataa
     2101 ccggcacacg ctagacttca gaggaattaa atacttccga agtctgaatt gagtggagga
     2161 taagatgcgc ggttttcgaa ttgtcatttt gctgagcgta ctcatctcga tcggagccgt
     2221 agtgccgatc gtgcaagctg ccagcacctg ttcgctcacg tgggatcgct atgatgccga
     2281 catacaagtc aacactgatg ggacaatgac ggtaacggaa attgaaacaa tccatttcac
     2341 cggatcgtgt cacaaaggtt tccgcgtcat tccgcaatcg cacggcacga cggacaacat
     2401 tgtcgtctgg gaaggcgatc ggcgttattc atctagcaat gccggcagtg agtactctta
     2461 tagcaccttt catgatgata acggcgacat caatcttacg tggtactttc cgtatacttc
     2521 caactcgaca catgtctaca acattcaata cacagtccag actggcttgc tgtattatcc
     2581 taaagatgac tacgatcgaa ttcaatggaa agcaattcca gccgatcacg cttatccgat
     2641 cttgaactcg aatgttacct tgcatctacc cgctagcgcg cctatcctca acgacaataa
     2701 tggcccgcgt atcggtgtgg cggatggaaa tctagcggtt gcgagtgctg gctccgatcg
     2761 atctacagcg cgattcgaag caatcagtcc attgtcacct ggtaacggta ttgaaattcg
     2821 cgccgacttc cagcatggcg cagtcgccgg gaccgcaccg gcttggcaaa cacagtatga
     2881 cttccaagat aaatatggcc cgatcatttc attagccggc tgcgtcttgg gattgctgct
     2941 gatgttgggc ggagtgttgt gggtgtatct gcgttggtat acacgcggac gtgatccact
     3001 aacgggtctc gtcaccgagt atgtgaccga accgccgagc gatctgcctg cgggaatggt
     3061 cggcacgttg gtcgatgaga aagctgacat gcaggacatc atcgccacgc tgatcgatct
     3121 ggcgcggcgg ggtagtatca ccatgcagga agagcaaacc gcgggcttcc tcggcatcgg
     3181 ttcatcgcgc gacttcatct tcaaacggat tgcaaattca aatgattcac agttgcgcga
     3241 ctacgagcgc actctgctca acgacgtgat accgtcgggc gagcgcaaac tatcggattt
     3301 gaagaacagc ttctatacat cgatcccggc gctcaaaaaa ggcttatatg cagaggttgt
     3361 caaagagggt ttgttcccgg ctagcccgga ttcggttcga tcgcgatggg cgggcgtcgg
     3421 ctgtgggatc attttcttag ctatcctcgg cgggattgtt gttggtgtat tgtttgccaa
     3481 taccgcgttt ggtgtggtct tccctttcat cgggttgggt ttcttcggcc tggtgacgat
     3541 gagcgcgagt gcggccatgc cggtcaagac gcgcaaagga tcggaagcaa aagctgcgtg
     3601 gctggccttc aagcgttatc ttgaaaatat caagcaatac accaaggtcg aaggagcgac
     3661 agcgcaattt gataagtatc tgccgtacgc gatcgccttc gggatcgatc ggagttggat
     3721 caatacgttc gcgcaagtca acacacctgt accgatttgg tattatccct atgggtacgg
     3781 tggaccgatc ggcagcggtt catcgatgtc aggcggtgga gttgggaaca tcggcacgcc
     3841 atccgtgcag ggcatgagca acagcctcgc cggttcgctg cagggtatga gcaccggact
     3901 cacatcgatg ttgaacag
//
LOCUS       DASRTJ010000462         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_256656,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000462 DASRTJ010000000
VERSION     DASRTJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256656"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFF70_09470"
     CDS             <1..260
                     /locus_tag="VFF70_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44963.1"
                     /translation="EGQDRWLQACAIYQMGFTSAYELIEHVQAALKSDDPLLRETAIA
                     AGRRLLDHQQYLQMLQEQSGNTDFPGVRRYALAQLEKLEPL"
     gene            257..715
                     /locus_tag="VFF70_09475"
     CDS             257..715
                     /locus_tag="VFF70_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZY44964.1"
                     /translation="MTLSTIERILFLRSAELFGQIASEDLVPVAQVAQEVHFNAGENF
                     IKQGDVGECLYLIVDGEASISLPGVGEVARRSAKEAIGEMAIISRQPRSADCQALTDL
                     TMLKIDYDEFWELLAEKPPLALGVIRTLAARLTEAVANLDRLSQKKIDSV"
     gene            712..1338
                     /locus_tag="VFF70_09480"
     CDS             712..1338
                     /locus_tag="VFF70_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZY44965.1"
                     /translation="MTSDLNFTGAIEYAVSRLGRELSPLLVYHTLWHTRDDVVPAVER
                     FAEMEGVTREDRLLLLTAAYYHDLGFVEKRVGHEDVSIRIVREVLPGFGYQPYQIDVI
                     ESIIRATQLPQTPQTLLQQIMADADLDALGRADFFERNQLLRQEWINFDLPSTEQEWD
                     EIQLSFLESHHYFTQAAINLRGLAEKRHIEILIDRIRRHGGRPIRDNT"
     gene            complement(1733..>2106)
                     /locus_tag="VFF70_09485"
     CDS             complement(1733..>2106)
                     /locus_tag="VFF70_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZY44966.1"
                     /translation="YTYTDVPSDMTQHDHDIFVTMGEAMAGMNSYTPKQASDLYITDG
                     TIDDWLYGVYHIYAFTFEMYPQSCSYPYYCGFYPPGSVIATQTERNREPILYAINVAG
                     NPDQVLPLQYPPRAYLPLVLK"
BASE COUNT          481 a          549 c          601 g          475 t
ORIGIN      
        1 ctgaaggtca agatcgctgg ctgcaagcct gtgcgatcta tcaaatgggt tttacatcgg
       61 cctatgaatt gatcgagcac gttcaagccg cgctgaaatc cgacgatccg ctgttgcgcg
      121 aaacggcgat agccgctggc aggcgcttgc tggatcacca gcaatacttg cagatgttgc
      181 aagaacaatc gggcaatacc gacttccccg gcgtgcgccg ctatgctctg gcacagttgg
      241 aaaaattgga gcctctatga ctttatccac tatcgaacgc atcctgtttt tgcggagcgc
      301 cgaattgttc gggcagatcg ccagtgaaga tttggtgccg gtggcgcaag tggcgcagga
      361 agttcacttt aatgcgggcg agaacttcat caagcagggc gatgtgggtg agtgcctgta
      421 tttgatcgtc gacggcgaag ccagcattag tttgccgggc gtcggtgaag tggcgcggcg
      481 cagcgccaaa gaagccatcg gcgaaatggc aatcatcagc cgtcaacccc gctcggcaga
      541 ttgtcaggcg ctgacggact tgaccatgct caagatcgat tacgatgaat tctgggaatt
      601 gctggctgaa aagccgccgt tggcgttggg cgtcattcgc acgctggccg cgcgtttgac
      661 tgaagcggtg gcgaatcttg atcgcttaag ccagaagaag atcgacagcg tatgacctct
      721 gatctgaatt ttaccggcgc aatcgaatac gcggtgagcc gtttggggcg cgagctttcg
      781 ccgctgctgg tctatcacac gttgtggcat acgcgcgatg atgttgtgcc ggctgtcgaa
      841 cggttcgccg aaatggaagg tgtcacccgc gaagatcgat tgttgctgct gactgccgcc
      901 tactatcatg atttgggttt cgtggaaaag cgggtcggac acgaagacgt cagtatccgc
      961 atcgtgcgtg aagtgctgcc cggcttcggt tatcagccgt accagatcga tgtgatcgaa
     1021 agtatcatcc gcgctaccca gctgccgcag acgccgcaga cgttgttgca acagatcatg
     1081 gccgatgccg atctggacgc attgggccgc gctgatttct tcgagcgcaa tcagctgctt
     1141 cgccaggaat ggatcaactt cgatctaccc agcaccgaac aggaatggga tgagattcaa
     1201 ctcagttttc tggaaagtca tcactatttc actcaagctg cgatcaactt gcgcggactc
     1261 gctgagaaac gtcacattga aattctgatc gatcgcatcc gccgccacgg tggaagaccg
     1321 attcgagata acacgtgacg gacaccgcat cgatattcct caccccgatc aacatgcaga
     1381 tcgatcagcg ccgcttccct tctccgagtc aatcgtcggg acagacctgg gtgtatgccc
     1441 attggatatt cgttgagcgt tctcattgga gaaaattaac gcatcgacat cgatcaaagg
     1501 tggaatgatg gccagccccc gccggtttca caatcgaggt ggaatcgatc gtgttcctcc
     1561 ccctccttcg actttgctac gcgccgctca ggaagtgggg gaggcgaatg gatcacatca
     1621 acaatcgatc aacagatggc gggggcacag accacatcag cctagaaggg cgagttgccg
     1681 acttgcccct actggctcac tactcacatg gacagactcg agtctcgact ctttacttta
     1741 gcacaagcgg gagataggcg cgcggtggat attgcagcgg caaaacttga tcgggattgc
     1801 cggccacatt gattgcgtac aagatcggct cgcggttgcg ttcggtctgg gtggcgatga
     1861 cgctgcccgg cggataaaat ccacaataat acggatagga acaagactgg ggatacattt
     1921 caaaagtgaa cgcatagatg tgatatacgc cgtatagcca atcatcgatc gtgccgtcag
     1981 tgatatacag atcgctggct tgtttcggcg tgtagctgtt catgcccgcc atggcttcgc
     2041 ccatcgtcac gaagatgtcg tggtcatgct gcgtcatatc agacgggacg tcggtataag
     2101 tgtaac
//
LOCUS       DASRTJ010000463         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_104426,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000463 DASRTJ010000000
VERSION     DASRTJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104426"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1988)
                     /locus_tag="VFF70_09490"
     CDS             complement(<1..>1988)
                     /locus_tag="VFF70_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZY44967.1"
                     /translation="WRESRRLILIEELIFFVAFAIFLFIRFGNPDLWHPAMGGEKPMD
                     FAYLNAVIKSQYFPPYDPWFAGGQITYYYYGFVLIATLIKLIGVIPSIAYNFAIPTLF
                     AMTALGAFCAGFNLIAERGMRYAEHSSLIAHHSPFSFRPLSFRNPIVIGLIAAVFVTF
                     MGNLGETQLLEEHISEIGQAQTHFNSSIHPLQMIVDTTAGLIRMATHGDQLNFRPEWW
                     YFTPTRIIPAPTTEAGPITEFPFFTFLYADLHAHMIAMPLTLLALALAIGWLKRIPQW
                     TWSGLGSIGLAALVVGALRATNTWDYPTYLVIGSAALLFGTFASESIGSLSTWIKVAI
                     RVGAFAGLSVIFFMPFSRNYATSYTSIEPWVGSLTPLWAYLNVHLLFLFPIVTFMLFE
                     FKRWGWRWWQHMWQLLKDWQLPILLLGAVVILAGVLVLFTGREVIILIEPIVILIMLL
                     IVRPRLSAVKRFWLFLILLSIGLTLVVEVVVLKGDISRMNTVFKFYIQVWILLGICAA
                     VALGWLADRLAAWRGWGGNLWKGFMFLLVVGSFLYVPLAARAKMLDRFVPDMPPGLNG
                     MDYMTRATYNETGQDFSLNADHDLITWLQDHAVGTPVIAEGQAPLYHWGARISINTGL
                     PSIIGWDWHQRQQRSIMPGSVIDNRLNDLRTLYQSTDI"
BASE COUNT          549 a          541 c          529 g          369 t
ORIGIN      
        1 gatgtccgtc gattgataca acgtccgcag atcattcagc cgattatcga tcaccgagcc
       61 aggcatgatc gatcgctgct ggcgctgatg ccaatcccag ccgatgatcg acggcaagcc
      121 cgtattgatc gagatgcgtg cgccccagtg atagagcggt gcctgccctt cggcgatcac
      181 gggcgtgccg acagcgtgat cctgcagcca ggtaatcaaa tcatgatcag cgttcaacga
      241 gaaatcttgg ccagtttcat tgtaggtcgc acgcgtcatg taatccatgc cattcaaacc
      301 cggcggcata tccggcacaa atcgatccag catcttggcc cgcgcagcca acggcacata
      361 caagaacgat ccgacgacga gcaggaacat aaagcccttc catagattgc caccccaccc
      421 acgccacgcc gctaatcgat cggctaacca gcccaacgcg acggccgcac agatgcccaa
      481 caatatccag acttgaatat agaatttgaa gaccgtgttc atgcggctga tgtcgccttt
      541 caacacgacc acttcgacca ccagcgtcaa accgatcgat aggagaatca agaacaacca
      601 gaaacgcttg accgctgata agcgcggtcg aacgatcagc agcatgatca aaatgacgat
      661 cggctcgatc aaaatgatga cttcgcgtcc ggtaaacaaa actagcacgc ccgccaagat
      721 cacaaccgcg cccagcagca agattggcaa ttgccaatcc ttgagaagtt gccacatgtg
      781 ctgccaccag cgccaacccc agcgcttgaa ttcaaatagc atgaaagtga cgatcgggaa
      841 cagaaacagc aaatgaacat taagataagc ccacaacggc gtcaacgagc caacccacgg
      901 ctcgatgctg gtgtaagaag tcgcataatt gcggctaaat ggcatgaaga atatcacact
      961 taaaccagcg aacgcgccaa cgcggatcgc gaccttgatc caagtcgaaa gagatccgat
     1021 cgattcactt gcgaatgtgc cgaacagcaa agcagccgag ccgatcacga ggtaggtcgg
     1081 atagtcccac gtattcgtcg cgcgaagtgc gcccacgacg agcgcagcga gcccgatcga
     1141 tccgaggccg ctccacgtcc attggggaat gcgcttcagc caaccgatcg ctaaagctaa
     1201 cgcgagcagc gtcaatggca tggcgatcat atgcgcatgc agatcggcgt acaagaaagt
     1261 aaagaatgga aattcagtga tcgggccggc ttcggtggtt ggcgcgggaa tgatccgcgt
     1321 cggtgtgaag taccaccact ccggcctgaa gttcaattga tcgccatgtg ttgccatgcg
     1381 gattaagccc gccgtcgtat cgacgatcat ttggagcggg tggatgctgc tattgaaatg
     1441 ggtttgcgct tgaccaattt ccgagatatg ttcttcgagc aattgtgtct cgccgaggtt
     1501 acccatgaac gtgacaaaga ccgcggcgat gaggccaatg acgatgggat tgcggaacga
     1561 gagcggacga aaactgaatg gtgagtgatg agcaatgagt gaactgtgct ccgcatatcg
     1621 cataccgcgt tccgcaatca ggttgaatcc cgcgcagaag gcaccgagtg cggtcatggc
     1681 gaacaaggtg ggaatcgcaa agttgtaagc gattgaggga atgacgccga ttaatttaat
     1741 cagcgtggcg atcagcacga agccgtagta ataataagtg atctgcccgc ccgcgaacca
     1801 cggatcatac ggtgggaaat attggctttt gatcaccgca ttgaggtagg cgaagtccat
     1861 cggcttctcg ccgcccatcg ctggatgcca caggtcggga ttgccgaagc ggataaacaa
     1921 gaagatggca aacgcgacaa aaaagatcaa ctcttcgatc aaaatcaaac ggcgtgattc
     1981 gcgccaga
//
LOCUS       DASRTJ010000464         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_353168,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000464 DASRTJ010000000
VERSION     DASRTJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353168"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3003
                     /locus_tag="VFF70_09495"
     CDS             <1..3003
                     /locus_tag="VFF70_09495"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY44968.1"
                     /translation="VLSISGTKLISNAVTGPGGGVYSSQSLAIINGYFERNRSNDQGG
                     GLRSNGSVTTTNTIFISNTADQGGGLHIDGAATLNTTQFSSNLAVVDDGGGAYIGGTA
                     SVTGSYFILNIAANHGGGAYFFGTSSVTASTFTTNTGDSGGGALFNGPASVNASSFIS
                     NTVFDLGGGAYFDGASNIANSSFVRNSTPYDGGGAYFFMPVTLTASTFTSNAADQGGG
                     AYFNNTVIMSGGIFARNVVTSAGAGLFTNNTLILSGTQFLTNTSQGSGGGLFATQAIT
                     LTNAQFISNTAKNGSGGVGTTGSIVLNGGYFERNVARNGVGGGVATISSLTINGSIFV
                     TNSSSIWGGAAFSIGPAQLIGGRYQANRAIGIFDAQGGALYISNTLSISGSQFMNNTA
                     HVEGGGVYAALTATLINAQFISNTSQAAGAGGLMAAQRLIMTNSQFLSNTSAYEGGGA
                     YVQDHVFGTSNLFRNNTSQTAYGGGLYLFGTGVISHSAFMNNTAQNNGGAIYITDLMD
                     TSVSADVYLINSQIIRNSSLNGSGGGVYSELPLETINTQFISNTARQNGGGLAVTADA
                     TIYDSEFRANTATLGIGGGVWIDSQLMMSETDVISNAATGKGGGLYAGGPTMFIGGPT
                     VIIGGRFERNDSLDAGGGAFISSTVSLTHVEFISNTGRGAFPFGGGGLYIYGTAAIDG
                     GLFENNRNLTQNGGGLFANNALSLNGTSFISNSTLLDGGGALIVGPALINNARFERNS
                     SLVDVAGGLYTFAAARVTNTLFFSNVAVNYGGGIYAVGSLDVRGGVFRNNRSLIRDGG
                     GLFATSSVGLTGTQFISNSARSGGGVYQRGVGASATGRVVNSLFVHNTAAITGAAMTI
                     ENSQNTVLLHDTIADLSLNPKPAISIVSGTVGITDTIIASHSIAISNTRGTVYEDYNL
                     LYSDPITRAGSISGGTHDGFGDPKFADPSIDNFHILTGSAAIDKAITAGVSIDFDGQA
                     RPFGAGFDIGYDEYVILKVYLPLALKNY"
     gene            3053..3751
                     /locus_tag="VFF70_09500"
     CDS             3053..3751
                     /locus_tag="VFF70_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZY44969.1"
                     /translation="MRIADMNWSQVEEYLKHEDRCVLPIGSTEQHSFLSLSTDSLLAE
                     RVSIEAAELIGVAVFPVVAYGLTPYFMAYPGTITLTESTYFALLTDILNSLVQHGFKR
                     IIIVNGHGGNSPAQVTCDRFANERKIQLKWHNWWNAPRTWAKVQSIDPVASHASWMEN
                     FPWTRLVNASMPNDQKKMIDPAHVKTLNPIEARKYLGDGNYGGVYQRSDEELLALWQI
                     GVEETRAMIEEDWK"
     gene            complement(3789..>4074)
                     /locus_tag="VFF70_09505"
     CDS             complement(3789..>4074)
                     /locus_tag="VFF70_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZY44970.1"
                     /translation="EPGTVKDVEMHDGSIIRLTKIGHDYDPTDRMAAIKLLEEAKAKQ
                     EFVTGLLYVNEGRPTLVEHEKLVATSLVHLPPEKLRPSKAALDKLNAEYM"
BASE COUNT          936 a         1112 c         1142 g          884 t
ORIGIN      
        1 gtgttatcga tcagcggcac aaaattaatc agcaatgcag tcaccgggcc gggcggcggc
       61 gtatactcca gtcaaagtct agcgatcatc aacggttatt tcgagcgtaa tcgcagcaac
      121 gatcaaggcg gcggcttgcg ctcgaatggc agcgtgacaa cgacgaatac gatctttatt
      181 agcaacactg ccgatcaagg cggcggactg catatcgacg gcgcggccac gttgaacact
      241 acgcaattca gcagcaacct cgccgtcgtc gatgatggtg gcggggcata catcggcggc
      301 acggccagcg taactggaag ttacttcatt ctcaacattg cagccaatca cggcggcggc
      361 gcatacttct tcggcacatc cagcgtaacg gccagcacct tcaccacgaa caccggcgac
      421 tctggcggcg gcgcactctt caacggtcca gcgagtgtga atgcttcctc atttattagc
      481 aacaccgtat tcgatctggg cggcggcgca tacttcgacg gtgcatctaa tatcgccaac
      541 agttccttcg tccgaaactc aacgccctac gacggcggcg gcgcatactt cttcatgcct
      601 gttacactga ccgcttcaac attcaccagt aatgctgcgg atcaaggtgg cggggcgtac
      661 ttcaacaata ccgtgatcat gtccggcgga atttttgcgc gcaatgttgt gacctctgca
      721 ggcgcaggct tattcactaa caacacactt attttgagcg ggacgcaatt tttaacgaac
      781 accagccaag gcagcggcgg cggattattc gcaacgcaag cgatcacact caccaacgca
      841 cagttcatca gcaatacggc caaaaatggc agtggcggag tcggcacaac cggttcaatc
      901 gttttgaatg gcggatactt cgaaaggaat gtcgcccgaa acggcgtagg tggcggtgtg
      961 gcgacgattt cttcgttgac gattaacgga tcgatcttcg tgacgaactc gtcttcgatc
     1021 tggggtggtg ccgctttctc gatcgggcca gcgcagctga tcgggggacg gtatcaagcc
     1081 aatcgggcga tcggaatttt cgatgctcaa ggcggggcgt tatacatcag caatacgcta
     1141 tcgatcagcg gcagccagtt tatgaataat accgcgcacg tcgaaggggg tggagtctat
     1201 gccgcattga ccgccacgtt gatcaacgca cagtttatca gcaacaccag tcaggccgcg
     1261 ggcgcgggcg gactgatggc agctcagcga ttgatcatga cgaactcaca gttcctgagc
     1321 aatacctcgg cttacgaagg cggcggagct tacgttcaag atcatgtgtt tggcaccagc
     1381 aacctgtttc gtaacaacac cagtcaaaca gcctacggcg gcgggctata tctatttggc
     1441 actggagtga tcagccattc ggcctttatg aacaacacgg cccaaaacaa tggcggcgcg
     1501 atctatatta ctgatctaat ggatacttcc gtaagcgccg acgtttattt aatcaacagc
     1561 cagatcatcc gcaacagttc gctgaacggc agcggcggcg gcgtgtattc cgagctgccg
     1621 ctggaaacga tcaatacaca gttcatcagc aacaccgccc gtcaaaatgg cggtggatta
     1681 gccgtgaccg ctgacgcgac aatctatgac agcgagttca gggcgaacac agcaacgcta
     1741 ggcatcggcg gcggtgtgtg gattgactct caattgatga tgagtgaaac agatgtgatc
     1801 agcaacgcgg ccaccggcaa aggcggcgga ctatatgctg gcggcccgac gatgttcatc
     1861 ggcggcccga ccgttatcat cggcggacgt tttgagcgga atgacagttt ggacgccggg
     1921 ggtggcgcat tcatcagcag cacggtcagc ttgacccatg tcgagttcat ctccaatacc
     1981 ggacgcggtg cctttccgtt tggcggaggc gggctgtata tttatggcac ggcggcgatc
     2041 gatggcggcc tctttgaaaa caatcgcaac ctgacacaga acggcggcgg tctgttcgct
     2101 aataacgcgc tgtccctcaa cggcacaagc ttcatcagca actccacgct gcttgatggt
     2161 ggcggcgcac tgatcgtcgg cccggccttg atcaacaacg cgcgctttga gagaaatagc
     2221 agtctcgttg atgtggccgg tggcttgtat acattcgctg cggcgcgtgt gaccaacacc
     2281 ttgttcttca gcaacgttgc ggttaattat ggcggcggaa tatacgccgt gggcagcctc
     2341 gatgtgcgcg gcggcgtgtt ccgcaacaac cgcagcctga ttcgagatgg cggaggttta
     2401 tttgccacta gctcagtggg tttgacgggt acacagttca tcagcaactc ggcgcgatcg
     2461 ggcggcggcg tgtatcaacg cggcgttggc gcaagtgcca ccgggcgagt ggtcaactcg
     2521 ctcttcgtgc acaacaccgc tgccatcacg ggcgcagcca tgactatcga gaacagccag
     2581 aacaccgtcc tgctgcatga tacgatcgcc gatctcagcc tcaaccctaa accggcgatc
     2641 tcgatcgtca gcggcacggt cggcattact gatacgatca ttgccagcca ttcgatcgcg
     2701 atcagcaaca ccagagggac ggtgtacgaa gattacaatt tgttgtattc cgatccgatc
     2761 acccgcgccg gatcaatttc cggcggcacg cacgatggtt tcggcgatcc gaagtttgcc
     2821 gatccgtcga tcgacaattt ccacatcctc accggctcgg cggcgatcga caaagcgatc
     2881 actgccggcg tctcgatcga tttcgacggc caagcgcggc ccttcggcgc gggcttcgac
     2941 atcggttacg atgaatatgt gatcttgaaa gtttatctgc cgcttgcgtt gaaaaattat
     3001 taattcgtag aggcgacccg ctgggtcgcc tttgttttac agaggaaaac ctatgcgcat
     3061 cgccgacatg aactggtcgc aggtcgaaga atatttgaag cacgaagatc gatgtgtctt
     3121 gccgatcgga tcaaccgagc agcattcatt tctcagcctg agcaccgaca gccttttggc
     3181 ggagagagtt tcgatcgaag ccgctgaact gatcggtgtg gcggtgtttc cggtggtggc
     3241 ttacggcctg acgccatact tcatggcgta tcccggcacg atcacattga ctgaaagcac
     3301 ttacttcgct ttactcaccg atattctcaa tagtttagtg caacacggct ttaagcggat
     3361 catcatcgtc aacggacacg gcggcaactc gcccgcgcaa gttacgtgcg atcgatttgc
     3421 caacgagcgc aagattcaac tcaagtggca caactggtgg aacgcgccgc gcacgtgggc
     3481 gaaggtgcag tcgatcgatc cggtcgcgtc gcatgcgtcg tggatggaaa attttccgtg
     3541 gacacgcctg gtgaacgcga gtatgccaaa tgatcaaaag aagatgatcg atccggcgca
     3601 cgttaaaaca ctgaacccga tcgaggcgcg caagtatttg ggcgatggaa actacggggg
     3661 cgtgtaccag cgatcagatg aagagctgct ggcattgtgg cagatcggtg ttgaggaaac
     3721 gcgggcgatg attgaagagg attggaaata aaaacgcggc gcacctttga agtgcgccgc
     3781 gctcgatatt acatatactc ggcgttgagt ttgtccagcg ccgctttgga cgggcgcaat
     3841 ttttcgggcg gcagatgaac caatgaggta gccaccaatt tctcgtgctc gactaaagtc
     3901 ggccggcctt cattcacata caacaggccc gtcacaaatt cctgtttggc tttagcctct
     3961 tccagcaact tgatggccgc catgcgatca gtgggatcgt agtcgtggcc gatcttcgtc
     4021 aagcggatga tcgatccgtc gtgcatctct acatctttga ccgtacccgg ttca
//
LOCUS       DASRTJ010000465         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_67336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000465 DASRTJ010000000
VERSION     DASRTJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFF70_09510"
     CDS             complement(<1..824)
                     /locus_tag="VFF70_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZY44971.1"
                     /translation="MPRFPFIAIEGVIGVGKTTLARMLTPDFGGDSLLEVFEENPFLS
                     DFYADRERYAFQTQIFFLLSRYRQQHQAVPNALKRGALFSDYTFAKDSLFAHLNLSGD
                     ELVVYEKLHSALAEKIPAPDLLIYLRADLDTLMTRIAMRDRPYERGMDRSYIDSLRLA
                     YEGFFTAYNSSPVLVIDTNSLNIVAKHEAYFNVRERIRSALGEGTFQPSLPQMPITAG
                     IESTRAVKIHKSTASSNPLQEFASLTQSIGNLGAVLSDGADASVLQESLQTILKRV"
     gene            966..1763
                     /gene="surE"
                     /locus_tag="VFF70_09515"
     CDS             966..1763
                     /gene="surE"
                     /locus_tag="VFF70_09515"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZY44972.1"
                     /translation="MSAQRPLILITNDDGINSIGLHAAAQVCERLGDILVVAPRQQQS
                     GAGRSMPPHSEGRIFHQYLEIDHQRVACYAVEGTPAQVVQHGLFEIAERKVDLVVSGI
                     NYGENLGEGVSVSGTVGAAMESASFRIPSIAISLETAKEHHLSLSAEIDFSVAAYFLS
                     IFVERTLKQGLPPKTDLLKIDVPTDATRQTAWRWTRLSQTRYFIPIKPHRRRPMDPGP
                     MGYEAHLDLEHLEKDSDVYAVRIDHLVSVTPITLDMTAPIDLQILPK"
BASE COUNT          443 a          505 c          518 g          439 t
ORIGIN      
        1 ttcacacgtt tcaagattgt ttgcagcgac tcttgtagca cactcgcatc ggcgccgtcg
       61 gacaagaccg cgccgaggtt cccgatcgat tgagtaagcg aggcgaattc ttgcagtgga
      121 tttgaagacg cggttgattt atgaatctta actgcgcggg tggattcgat accggcggtg
      181 attggcattt gaggtaatga gggttggaac gtgccttcac cgagggcaga tcgaattcgt
      241 tcgcggacat tgaagtaagc ttcgtgtttg gccacgatgt tcaggctatt ggtatcgatc
      301 acgaggacgg gtgaagaatt atatgccgtg aagaaccctt cataagccag gcgcagacta
      361 tcgatgtaac tgcgatccat gccgcgctca tacggtcgat cgcgcatggc gatgcgggtc
      421 atcaaggtat ccaaatctgc gcggaggtag atcaacagat cgggcgcggg aattttttcg
      481 gccagggccg agtggagctt ctcgtataca actagctcat cgcccgatag attcaagtgc
      541 gcgaataagg aatctttggc gaaggtgtaa tcgctaaata atgcgccacg cttcagcgca
      601 ttgggcacgg cttgatgctg ctgccggtag cggctgagca aaaagaaaat ctgcgtttga
      661 aaagcgtagc gttcgcgatc ggcgtagaag tcgctgagaa acggattttc ctcaaagact
      721 tcaagcagcg agtcgccgcc gaagtcaggc gtcagcattc gggctaaagt cgtcttgccg
      781 acgccaatga caccttcaat cgcgatgaaa ggaaaacggg gcatgcttct ccaattaacc
      841 ctgtgcacta acctgctgag cttaatggcc ttgcaaataa atgtcaagac gctgatcgat
      901 caagtggcac tttgttctcc agctgtcact cgccggatca tgtgcttcag tttataatgc
      961 tgaacatgtc tgctcagcgc ccgctgattt taatcacgaa tgatgacggc atcaactcga
     1021 tcggtctgca cgccgccgcg caagtgtgcg agcggcttgg cgatatcttg gtcgtggcgc
     1081 cgcgtcaaca gcaatcgggc gcaggccgat cgatgccgcc gcattcggaa ggccgcatct
     1141 tccatcagta tcttgagatt gatcaccaac gtgtcgcgtg ttacgccgtc gaaggcacgc
     1201 ccgcgcaagt cgttcaacac ggtttgttcg agatcgccga gcgcaaagtt gatctggttg
     1261 tgtccggcat caactatggc gaaaatttgg gcgaaggcgt ctcggtgtcc ggcaccgtcg
     1321 gggctgccat ggaatccgct tcatttagaa ttccatcgat cgcgatctcg ctggaaaccg
     1381 ccaaagaaca tcatctcagc ctatcggcag agatcgattt ttccgtggcc gcatattttc
     1441 tatcgatctt tgtggagcgc acattgaagc agggcctgcc gcccaaaact gatctgttga
     1501 agatcgatgt gcccaccgac gccactcgtc aaacggcttg gcgctggacg cgcttaagcc
     1561 agacgcgcta cttcattccg atcaaacctc accgccgtcg accgatggat cccggcccga
     1621 tgggctacga agcgcacctc gatttggagc atctcgaaaa agattcggac gtgtatgccg
     1681 tgcggatcga tcacctcgtg tcggtcacac ccattacgct ggacatgacc gcgccgatcg
     1741 atttgcagat attgccgaag taaaccgtgc cagatggttc caaacctcct caacctcgag
     1801 gacgcgtttc ctcacgtaag aacctgaccg aaacgctatt cgtttcctca cgaaaaagct
     1861 gaccgaacga gtcagtcgtt tcctcacttt aaagctaact gaagg
//
LOCUS       DASRTJ010000466         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_29425,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000466 DASRTJ010000000
VERSION     DASRTJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29425"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFF70_09520"
     CDS             <1..582
                     /locus_tag="VFF70_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZY44973.1"
                     /translation="VLVRTRTDRSRRELERKLGWPLHTLEDRVFNGELSIRASHGEIT
                     EEAFWDQAARDFNLAQYQMTGADFRHEFFVDDFLDEELVQFMRGLRPAIKVGLISNAW
                     LGLRSVLQTVFHIDDMFDEIVISAEENVMKPDARIYRLALDRLGLQPAETIFLDDMPI
                     NVEAADAVGMLGVRFQSTAQALRDIRSRLNHHV"
     gene            585..1115
                     /locus_tag="VFF70_09525"
     CDS             585..1115
                     /locus_tag="VFF70_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HZY44974.1"
                     /translation="MAIIGIVADTHVPQRLKQLPPGIFAALRGVDLILHAGDINSRQV
                     LRELEEIAPVQAVIGNADLFRTSLPLTRLIEIEGQRIGLVHGHGGWPLYVWSKLRDQL
                     GYNQEYYLRGVRRSFGPVDAIVFGHTHRFYRSNQAGVLLFNPGPIAPEYYNTSGPQIG
                     KLHVSRESIQTEIVEL"
     gene            1207..1986
                     /locus_tag="VFF70_09530"
     CDS             1207..1986
                     /locus_tag="VFF70_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZY44975.1"
                     /translation="MDRVLISVEHYDDMAAYLPVVQENGLGLELQEFADPNVLDNDWR
                     GLVRKYQRALSDFHGTLTLHGCYIDLVSGSPDKKLVALTRERYQLNIEIGRQLGATAI
                     DFHANYLPLIDQPSYLAGWLDRQVAFWSVLAEESAKHDIMLLLENMWEPDPSIIKHIL
                     MRIKSPQLKACLDVGHAGLYSALPLETWISSLGADLVYTHLHNNNGQHDEHLAFGIGV
                     IDFPALLKRLREIPRPPVFTLEMPNLETIKASLPFLNLDKR"
BASE COUNT          432 a          546 c          576 g          472 t
ORIGIN      
        1 gtgctggtgc gcacccgaac cgatcgatcg cggcgcgagt tggaacgcaa gttgggttgg
       61 ccgctgcaca cccttgagga tcgcgtcttc aacggcgagc tgtcgatccg tgcctcacac
      121 ggtgaaataa cggaagaagc cttctgggat caagcagccc gcgattttaa tttggcgcaa
      181 tatcaaatga ccggcgctga tttccgtcac gaattctttg tagacgactt tttagatgaa
      241 gagttagtcc aattcatgcg cggcctgcgc cctgcgatca aagttggctt gatcagcaat
      301 gcctggctgg gtttgcggtc cgtattgcag acggtgtttc acattgacga catgtttgat
      361 gagatcgtca tctccgctga agaaaatgtg atgaagcccg acgcgcgcat ctaccgcctt
      421 gcgctcgatc gacttgggtt gcaaccggct gaaacgatct tcctcgacga tatgccaatc
      481 aacgtcgagg cggcggacgc ggtgggcatg ctcggcgtgc gtttccagtc gaccgcgcag
      541 gcgctgcgcg acattcgatc acggttgaat catcacgtgt aagtatggcc atcattggca
      601 tcgtcgccga tacgcatgtg ccgcaacgcc tgaagcaatt gccgccggga atttttgcgg
      661 cgctgcgcgg cgtcgatctg attttacacg cgggcgatat caactccagg caggtgttgc
      721 gtgaacttga agagatcgcg ccggtgcagg ccgtaatcgg caatgctgat ctgtttcgta
      781 cgagcctgcc gttgacgcgc ttgatcgaga tcgaaggcca gcgcatcggt ttggtgcatg
      841 ggcacggcgg ctggccgttg tatgtctggt cgaagctgcg cgatcagctg ggttacaatc
      901 aagaatatta tttgcgtggg gtgcgtcgat cgttcgggcc agtggacgcg atcgtgtttg
      961 gccacaccca tcggttttat cgatcaaacc aagcgggcgt gctgttgttc aatccggggc
     1021 caatcgcgcc ggagtattac aatacatccg gcccgcagat cggcaaactg cacgtgtcgc
     1081 gcgaatcgat ccaaaccgaa atcgtcgagc tgtgatattt ggtctgcagg agcgaacggg
     1141 cttaatcggt ttggcgaaca acttgaagtg ttgtaaaata tcgcaagttt gaaccggggt
     1201 ccgagcatgg atcgcgtact catttctgtc gaacactatg acgacatggc ggcctatctg
     1261 cctgtcgtcc aagaaaatgg attggggctg gaactccaag agtttgccga tcccaacgtc
     1321 ctcgacaatg attggcgcgg cctggtccgc aaatatcagc gcgcgttgtc tgactttcac
     1381 ggcaccttaa cgctgcatgg ctgctacatc gatttggtta gcggcagccc cgacaagaaa
     1441 ctcgttgctt taacccgtga acgctatcaa ctcaacatcg agatcggccg gcagctcggc
     1501 gcgacggcga tcgattttca cgccaactat ctaccgttga tcgatcagcc gtcatatctg
     1561 gccggctggc tcgatcggca ggtggcgttc tggtcggtgt tggctgaaga gtctgccaag
     1621 catgacatca tgctcttgct tgaaaatatg tgggagccgg atccgtcgat catcaagcac
     1681 attctcatgc gcattaaatc accgcagctc aaagcctgtc tggacgtcgg ccacgcggga
     1741 ctgtattccg ctctgccgct ggagacgtgg atctcctcgc tgggcgccga tctggtctac
     1801 acgcatctgc acaacaacaa cggccagcat gacgaacatc tggccttcgg catcggcgtg
     1861 attgattttc cggcgctgct caagcggctg cgcgaaattc ctcggccgcc ggtgttcacg
     1921 ctcgaaatgc cgaacctcga aacgatcaag gcgagtctcc cttttctcaa tttggataaa
     1981 cggtaaatca aaatttgatc gatcataaaa gcgcgactta gcaagc
//
LOCUS       DASRTJ010000467         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_201669,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000467 DASRTJ010000000
VERSION     DASRTJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201669"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VFF70_09535"
     CDS             complement(<1..1412)
                     /locus_tag="VFF70_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZY44976.1"
                     /translation="MSLVNKDAAGMRDHVTVMPLLPNKDEQRLLGFILIVFLLLTAIS
                     NFTIPLFEAPDEDDHYRFALWLAYGNGLPDLNQLVAQVGHEAWQTPLYYYLLSPVMRL
                     ANGGQPDEIAPRSVGYLAGYSIVTRIHSQAESWPYVGTSLAVHLARLITSLFGVGTIL
                     VTFGLARLIWPRAAWVAAALVAFNPQFIYLSAAISNDVPAACLAGLTMCWLVRGLHQD
                     SIGKEWNIVLGILAGLAVMTKLSNLVLVIPIGLALLLRLKPFRQTWRRILADSMLLLI
                     AFVSIAGWWFLSNWLRYHDWLAWQPMLNLSVGLQRGHLLNWFEALQVSAGLLTSFWLN
                     IGNGAPQFYLFFDVLMLAAAIGLGAWLWQQLRQHTRRVFVNVGLIAVWISLTFISLLQ
                     WMRLVRATDQGRLLYPVISGLAVLLAVGLMQIAWRKWSVAPVLVAGLFIAAALTPVLV
                     TMPAYAQPRPLPMGEQLPNPR"
     gene            complement(1412..2437)
                     /gene="pheS"
                     /locus_tag="VFF70_09540"
     CDS             complement(1412..2437)
                     /gene="pheS"
                     /locus_tag="VFF70_09540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZY44977.1"
                     /translation="MLDQLKSIEKDALNALRSIENKDALEVWRVEYLGKKSPLMTILG
                     DLGKLSKEERPSIGKRGNEVKNALETAYVDRVEAIRAAEMQHGLTTGALDVTLPGRPI
                     ERGRLHPATQTLREIYAIWAEMGFQVYRARDVETDEFNFQLLNIPPYHPARDMQDTFY
                     TTTPGVLLRTQTSPGQIHAMREYYPQPIRIIVPGMCYRYEQSSASKDIQFMQVEGLAV
                     GRNIRMTDLLGTMTEFARRIFGTDRKTRFRASYFPFTEPSMEVDVTCVVCGGSGCHVC
                     KHTGWLEIAGSGMVHPTVLRNGGYDPSIYSGFAFGMGPQRVTMLKYGITDIRYFWQND
                     LRFLEQF"
BASE COUNT          670 a          737 c          636 g          525 t
ORIGIN      
        1 cgcggattgg gaagttgctc acccatcggc agcggtcgag gctgagcata ggcaggcata
       61 gttaccagca caggtgtcaa tgcagccgca ataaataatc ctgcaacaag cactggcgct
      121 actgaccact tccgccaagc aatctgcatc aatccaaccg ccaacaacac ggccagtcca
      181 ctgatcaccg gatacaacaa tcggccttga tccgtggcac gcactagtcg catccattgc
      241 agcagactga taaatgttaa actaatccaa acagcaatca agccgacgtt cacgaaaact
      301 cttcgtgtgt gctgccggag ctgctgccat aaccaagcac ccaagccgat tgctgccgcc
      361 aacatcaaca catcaaagaa gagatagaac tgcggcgcac cattaccgat attgagccaa
      421 aacgaagtca acagacccgc actcacctgc aacgcttcga accaattgag taagtgccct
      481 ctttgtaatc cgaccgataa attcaacata ggttgccacg ccagccaatc atgatatctc
      541 aaccaattcg agagaaacca ccagccggcg atagacacaa aggcaatgag cagcagcata
      601 ctatcggcta atatgcggcg ccatgtttgc cgaaaaggct taagcctcaa gagcaaagcg
      661 agtccaatgg gtattaccaa gaccagattg ctcaactttg tcatgacggc caagcccgct
      721 aagatgccta aaacgatgtt ccattctttc ccaatcgaat cctggtgcag accccgcact
      781 agccagcaca tggtcagacc tgccaaacaa gcggcgggca catcattgct gatcgccgca
      841 ctcagataga tgaactgcgg gttgaaagcg acgagcgcgg ctgctaccca cgcagcccta
      901 ggccagataa ggcgtgccag accaaaagtg accagaattg tgccgacgcc gaataagctc
      961 gtgatcagcc gcgccaaatg aacggccagg ctcgtaccaa catacggcca cgactctgct
     1021 tggctatgaa tacgcgtcac gatcgaatag cctgccaggt atccgacgct gcgaggcgca
     1081 atctcatcgg gctggccacc gttcgccaaa cgcataacag gactaagcaa ataatagtat
     1141 aacggtgtct gccatgcttc gtgtccaact tgggcaacga gctggttgag atcaggtagg
     1201 ccattgccgt aagccagcca taatgcaaat cgataatgat catcttcatc gggtgcttca
     1261 aacaacggaa tagtaaagtt cgaaatggct gtgagcagta aaaaaacaat caaaataaag
     1321 cccaacagac gttgctcatc tttattgggc agcagaggca ttaccgtcac atgatctcgc
     1381 atgccagctg catctttatt caccaaagac attagaactg ctccaagaac ctcagatcgt
     1441 tctgccagaa atagcggatg tcggtgatgc catatttcaa catcgttacg cgctgcggcc
     1501 ccatgccgaa cgcaaagccg ctgtagatcg atggatcgta gccgccattt ctcaacacgg
     1561 ttggatgcac catgcccgat ccggcgatct ccagccagcc ggtatgcttg cacacatggc
     1621 agcctgatcc gccacagacc acgcacgtca catcgacttc catgctcggt tcggtgaacg
     1681 ggaaataaga ggcgcggaag cgcgtcttgc gatcggtgcc gaagatgcgt cgggcgaact
     1741 cagtcatggt gccaagcaga tcggtcatgc ggatattcct gccaacggcc aaaccttcga
     1801 cctgcatgaa ttggatatct ttcgacgccg agctctgttc atagcgatag cacatacccg
     1861 gcacaataat ccggatcggc tgcgggtaat actcacgcat cgcatgaatc tgtcccggcg
     1921 atgtttgtgt gcgcaacaac acgcccggcg ttgtggtata gaaagtgtcc tgcatatccc
     1981 gcgccggatg atacggcgga atattgagca actgaaaatt gaattcatcc gtctccacat
     2041 cgcgcgcacg ataaacctgg aagcccatct ccgcccagat cgcataaatc tcgcgtagcg
     2101 tttgggtcgc tggatgcaag cgcccgcgtt cgatcggtcg accgggcagt gtgacatcga
     2161 gcgcgccggt cgtgaggccg tgctgcattt cggcagcacg gatcgcttca acccgatcga
     2221 catatgcggt ttccagcgcg ttcttcactt cgttgccgcg tttgccgatc gacgggcgct
     2281 cttctttgga caacttgccc aagtcgccga ggatcgtcat caacggcgat ttcttgccga
     2341 gatattcgac acgccaaact tcgagcgcat ccttgttctc gatcgagcgg agcgcgttga
     2401 gcgcgtcttt ttcaattgat ttgagttgat caagcatgtc agcctccatg aaaataaatc
     2461 tgggagtggt gtgtcactgc gagcctcgca gaggcgaagc accacttgcg ccgtaatgcg
     2521 gagcagtgca ggtgtttccc gggcaatcaa ctgagattgc ttcgccgc
//
LOCUS       DASRTJ010000468         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_164074,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000468 DASRTJ010000000
VERSION     DASRTJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164074"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1286
                     /locus_tag="VFF70_09545"
     CDS             183..1286
                     /locus_tag="VFF70_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY44978.1"
                     /translation="MRNIFPFSAIVGQERMKRALVLNAVYPQIGGVLIRGERGTAKST
                     AARALAALLPDLDVVVDCRFGCDPDQPATWCTECRERANGNGTGLPRTTRKTKFVDLP
                     VSATEDRVVGTLDIEQAIQKGIRRFEPGVLASANRGLLYVDEVNLLDDHVVDLLLDSA
                     AMGVNVVEREGISFSHPSRFILVGTMNPEEGDLRPQLLDRFAFAVDIQGIHDQMARMQ
                     ILERNLQFESDPEGFYTEWEPREKLLSQEIDRAQKTLPEVQYRQSDLASIAGLVGSLG
                     VDGHRADIVILKGARAHAALAGRRHINKEDILLAAELALPHRLKKQPFQEDGSVSTQD
                     LSERLDRAESEFPGGDDVEMDPDGEEGGTKKKR"
     gene            1421..2251
                     /locus_tag="VFF70_09550"
     CDS             1421..2251
                     /locus_tag="VFF70_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY44979.1"
                     /translation="MRKLDTPLDKLTRRSAGRRSTSKTDRKRGRYIQARPSPGNPTDL
                     AFDATLRAAAPFQNERKAEVADGPALRVKRGDYQKKVRVRKAANLILFIVDASWSMAV
                     AERMAATKGAIMSLLTDAYQRRDRVGLIVFQKDQARLILPPTNSVDLARKALRDIPVG
                     GKTPLSAGLTLTHMVLVQELRLHPDIAPLAIILTDGAGNVSQSGSTPAQEESYRVAGQ
                     IAKMDIRTVVINMEHPAFDQGLAQKLADHLEAPCYTLKELKAESLYRTVRDELDSPKM
                     "
     gene            2259..2795
                     /locus_tag="VFF70_09555"
     CDS             2259..2795
                     /locus_tag="VFF70_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44980.1"
                     /translation="MYSIQRETLSPDQPIRYTVTDDAGEGLYIVERTNELLDLNQQRA
                     RILDRAGHPVAQLIPPAERNFWRATSLYQLTMAGEEQAHFTIEQTYSLIDRILLRLPH
                     YKLRGHGARFNARGSRYSEHFYELFGAKDKFLGRIVRPSHGPTYLIEGEPSPLMQMPL
                     LLSALTIVVDLQMADSDG"
     gene            2825..>3280
                     /locus_tag="VFF70_09560"
     CDS             2825..>3280
                     /locus_tag="VFF70_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44981.1"
                     /translation="MQNQNSIVQPASARLSVQVKFENSRGILEAPRGTLLKEFIRGAY
                     PDKQRSIVAAFINNKLSELSTPIETDCSVRPIDLTSRDGMRIYRRSLMFLLIAAAREL
                     FPTANIYIDHSLPFGGYYCEVTGRDPLTLDELMALQSRMREIVNADEPIV"
BASE COUNT          737 a          912 c          940 g          691 t
ORIGIN      
        1 gacgcaggcg atctatactt gctactaccg attgttcttg ccattggctt tgaaggattt
       61 ctaaatcgat cgatttcaaa attacccgat caagaaaacc tcctccatcc tgaggaggtt
      121 tttgtttctc gatgagtggt ataatctcag cggatttcac ttggaaaggc tactctttta
      181 gtatgcgaaa catttttcct ttcagcgcga tcgtcggcca agagcggatg aagcgtgcct
      241 tagtcttgaa tgccgtctat ccgcagatcg gcggggtgct gattcgcggc gagcgcggca
      301 cggccaaatc gacggcggcg cgcgcgttgg ccgcgctgtt gcccgatcta gatgtggtcg
      361 tcgattgccg tttcggctgc gatcccgatc aacccgcgac gtggtgcacc gaatgccgcg
      421 aacgcgcgaa cggtaatggc acaggcttgc cgcgcaccac ccgcaaaacg aagttcgtcg
      481 atctaccggt cagcgccacg gaagatcggg tcgtcggcac gctcgatatt gaacaggcga
      541 tccaaaaagg catccggcgt tttgagccgg gcgtgttggc ttcggcgaat agaggcttgt
      601 tgtatgtcga tgaagtgaac ttgctcgacg atcacgtggt cgatctgctg ctcgactcgg
      661 cggcgatggg cgtcaacgtc gtcgagcgcg aaggcatttc atttagtcat ccgtcacgat
      721 ttattttggt cggtacgatg aatcctgagg agggcgatct caggccgcag ctgctcgatc
      781 gtttcgcgtt cgccgtcgat attcaaggca ttcacgatca aatggcgcgc atgcaaatcc
      841 ttgagcgcaa tctgcaattc gaaagtgatc ccgaaggttt ctataccgaa tgggaaccgc
      901 gcgaaaaact tttgagccag gagatcgatc gggcgcagaa aactctgccc gaagttcaat
      961 atcgacaaag cgatctggct tcgatcgccg gactcgtcgg cagtctgggc gtagatggac
     1021 atcgcgccga tatcgttatc ctgaaaggtg cgcgggcgca tgccgcgttg gcggggcgta
     1081 ggcatatcaa caaagaagat attttgttgg cggctgaact ggcgctgccg catcgcttga
     1141 agaaacagcc tttccaggaa gacggatcag tttccacgca agacttgagc gagcggctcg
     1201 atcgagcgga aagcgaattc cccggcggcg acgatgtgga gatggatccc gacggcgaag
     1261 aaggcgggac gaaaaaaaaa cggtagcgcc cgaaggcgaa gtcaaagaca cggctgactt
     1321 cgggcagagc gatgctcgca ccaaatctca aacgcaaggc acgcaggacg cttactggtg
     1381 ggaaggcggc gatcaagttc ccatcggcga gcagttcgat gtgcgcaagc tcgatacacc
     1441 gctcgataag ttgacgcggc gatcggcggg ccggcgcagc accagcaaga ccgatcgcaa
     1501 acgcggacgt tacatccagg cccgaccgtc tcccggcaac cccaccgacc tcgcgttcga
     1561 tgcaaccctc cgcgcggcgg cgcctttcca aaacgaacgc aaagcagaag ttgccgatgg
     1621 cccggcgctg cgggtgaagc gcggcgatta tcaaaagaaa gttcgagttc gcaaagcagc
     1681 caacttgatt ttgtttatcg tcgatgcgtc gtggtcgatg gcggtggccg agcgcatggc
     1741 cgccaccaag ggcgcgatca tgtcgctgtt gacggatgcg tatcagcggc gcgatcgagt
     1801 ggggctgatc gtttttcaaa aagatcaagc gcgcttgatt ctgcccccga cgaactcggt
     1861 tgatttagcc cggaaggcct tgcgcgatat tccggtcggc ggcaagacac cactctcggc
     1921 gggactaacg ttgacgcaca tggtgctggt tcaagagctg cgcctgcatc ccgatatcgc
     1981 gccactggcg atcattctca ccgatggcgc ggggaacgtc tcgcaaagcg gatcgactcc
     2041 ggcgcaagag gaatcctatc gagtggcggg gcagatcgcc aagatggata ttcgcacggt
     2101 ggtgatcaac atggaacatc ctgcgttcga tcaagggctg gcgcagaagt tggcggatca
     2161 tctcgaggcg ccgtgctata cgctgaaaga actgaaggct gaatcgctct atcgcacggt
     2221 gcgcgatgag ctcgatagcc cgaagatgta aattgctcat gtattccatt caacgcgaaa
     2281 ccctgtcacc cgatcaaccg atccgttaca ccgtcaccga tgatgcaggc gaagggttgt
     2341 atatcgtcga gcgcacgaac gaactgctcg atctgaatca gcagcgtgcg cggattctcg
     2401 atcgcgccgg tcatccggtc gcgcagctca tcccgccggc agagcgcaat ttctggcgcg
     2461 cgaccagcct gtatcagcta accatggccg gtgaagagca ggcacacttc acgatcgaac
     2521 aaacttattc gctgatcgat cggattctgc tgcggctgcc gcattacaag ctgcgcggtc
     2581 acggtgcccg tttcaatgcg cgcggcagcc gttacagtga gcacttctat gaactctttg
     2641 gtgcgaaaga taaattcctg ggccggatag tgcgcccctc acatggaccc acttacttga
     2701 ttgagggcga gccttcacct ttaatgcaga tgccgctgct gttgtccgcg ctgacgattg
     2761 tcgtcgattt gcaaatggca gacagcgacg gctaatcctc tatctcctaa atcgtttcat
     2821 cactatgcag aatcaaaact caattgtcca acccgcctca gcgcgtctca gcgtacaagt
     2881 caaattcgag aacagccgcg ggattttaga agcgccgcga ggcacgctgc tgaaagaatt
     2941 cattcgcggc gcctatcccg acaagcagcg ttcgatcgtc gccgcgttca tcaacaataa
     3001 attaagcgaa ctctcgacgc caatcgaaac cgattgcagc gtgcggccga tcgatctcac
     3061 cagccgcgac ggcatgcgta tctatcgccg ctcattgatg tttctgctga ttgcagccgc
     3121 gcgcgaatta tttccaactg ccaatatcta catcgatcat tcgctgccgt tcggcggcta
     3181 ttattgtgaa gtcaccggac gcgatccgtt gacgcttgat gaattgatgg cgctccaatc
     3241 gcgcatgcgc gagatcgtca acgccgacga accgatcgtg
//
LOCUS       DASRTJ010000469         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_277729,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000469 DASRTJ010000000
VERSION     DASRTJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277729"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFF70_09565"
     CDS             complement(<1..1136)
                     /locus_tag="VFF70_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZY44982.1"
                     /translation="MSSDIEIVECTTPQQRDEFIFFQWVPYKSNKYWVPPLISERREF
                     YNKDKHPFHKHAEVSMFLAKRNGETVGTTYAIENYKHNEFHTEKIGMFGGFETIDDQA
                     VANALLSKAAEWCKARGLTALRGPFNFSTNEECGLLIDAFEDTPRPLMTYNPPYYQKL
                     IETAGLKKAMDLLAYNLEVKDIQKGVDALPAKLKRVTDMLMKRNEIVIRKVEMKHLDA
                     EIELLKKVYNSAWENNWGFVPMTEEEFEHLANGLKPVLDPDLIFIVEAKGKPIGFGLT
                     LPDVNVPLLYANPNPKTPEIFTLVRFMANLKLRRSMMKYVRVIALGVIKEYRLTGVDG
                     LLYRRTAEEVLKKGYNIGEFSWILETNAPMRNAIEGLGGKVYKT"
     gene            complement(1229..2665)
                     /locus_tag="VFF70_09570"
     CDS             complement(1229..2665)
                     /locus_tag="VFF70_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44983.1"
                     /translation="MFKIDPALCPKIFLRAVWLIPIVLAALIGVTTVRAVPIPHLGYG
                     FNVAEQDYTLLNNMGFDWIKYYNVPPIDPPPSWAHVLLRITVNKQSYNQLSTFLNDLD
                     YTLVYKDHVDAYELGNEVNLDANYGWNAAPDAQKYKTVLCAAYNKIKQADPTAIVVSA
                     GLAPTGRVTGSWNGHAGHNGAYQDEREFLKEIINAGGGSCLDAVGYHPYGFSADFNAP
                     PDIASTNPTLNCANGFCFRGVEKIYEIMQANGLSNKQIWPTEFGWITDPPDYCKSQPD
                     WSGRLWQIVTEQKQSSNLAGAFQYADAHYPWMGTMFVFNLNFNTSPYYDECEQMRFYG
                     VEGRPAQTALTNLTKNKVPGHLHVSPSYILMLIKPDWQPITQSFALNFNNTGWQTLRY
                     TVTANSNLTIVPTLANASRNLNPMITKSTTVTISSTDRITGSYTGALTVTASIGALGV
                     PITIPIELRVVDVIWQTYLPLISANSLP"
BASE COUNT          638 a          690 c          691 g          698 t
ORIGIN      
        1 taagtcttat acacctttcc gcctaagcct tctatcgcat tgcgcatcgg cgcattggtt
       61 tccagaatcc acgagaactc gccgatattg tagcccttct tcagtacctc ttccgcggtg
      121 cggcggtaca acaaaccatc cacccccgtt aagcgatatt ccttgatcac gcccaaggca
      181 atcacgcgta catacttcat catcgatcgg cgcagtttca agttcgccat gaatctcacc
      241 agcgtgaaaa tttccggcgt cttgggatta gggttggcat acagcagcgg cacattgaca
      301 tccggcaggg tcaagccaaa accgatcggt ttgcctttag cttcgacgat aaatatcaaa
      361 tcgggatcca gtacgggctt caagccattc gccagatgct cgaactcctc ttcggtcatc
      421 ggcacaaagc cccagttgtt ctcccaggct gagttgtaga ccttcttcaa caattcgatc
      481 tctgcgtcga gatgcttcat ttcgactttg cggatgacaa tctcattccg cttcatcaac
      541 atatcggtca cgcgttttaa tttggccggc agcgcgtcga cgcctttctg aatgtctttc
      601 acttccaagt tatacgcgag caagtccatg gctttcttca agccggcggt ttcgattaat
      661 ttctgataat acggtggatt gtaagtcatt aacggacgcg gggtatcctc aaacgcatcg
      721 atcagcaagc cgcactcttc gttcgtcgag aagttgaacg gcccacgcag cgccgtcaga
      781 ccgcgcgctt tgcaccactc ggccgcttta ctcaacagtg cattcgctac cgcttgatcg
      841 tcgatcgttt cgaagccacc gaacatgccg atcttctcag tgtgaaattc gttgtgcttg
      901 taattctcga tcgcgtaagt ggtgccgaca gtctcgccgt tgcgtttggc cagaaacatg
      961 gaaacttcgg cgtgtttgtg aaaaggatgt ttgtctttgt tatagaattc gcggcgttcg
     1021 ctgatcagcg ggggtaccca gtatttgttg cttttatatg gaacccattg aaagaagatg
     1081 aattcatcgc gctgctgggg agtggtgcat tcaacaatct cgatgtcgga tgacatgtaa
     1141 ttcgcctccg tgcctggatg cgcgtattat acccctctcc gttgtaaaga caaaaattgc
     1201 agggcggtga caaaattaag ttgacaactt acgggaggct gttggctgat atgagcggca
     1261 agtaagtctg ccagatcaca tccaccacgc ggagttcgat cgggatcgta atcggcacgc
     1321 cgagtgcgcc gatcgaggcg gtaacggtta gcgcgccggt atacgatcca gtaatgcgat
     1381 cggtgctgct gatggtcacg gtggtcgatt tggtgatcat cggattcaag ttgcggctgg
     1441 cattggccaa agtcggcacg atcgtgaggt tggaattagc cgtcacggtg taacgcagtg
     1501 tctgccagcc ggtattgttg aagttgagcg caaaactttg cgtgataggc tgccaatcag
     1561 gcttgatcaa catcaggata taactcggac taacgtgcag atgacccggt actttattct
     1621 tggtgaggtt cgtcaatgca gtttgcgccg ggcggccttc cacaccgtag aaccgcatct
     1681 gctcgcattc atcataataa ggtgacgtgt tgaagttgag gttgaagaca aacatcgtac
     1741 ccatccacgg gtagtgggcg tcagcatatt gaaaggcacc ggcaagattg ctggattgct
     1801 tctgttcagt aacaatttgc cacaagcggc ctgaccaatc tggctggctt ttacaatagt
     1861 ccggcggatc ggtgatccaa ccaaattcgg ttggccagat ttgcttgttg ctcaagccat
     1921 tcgcctgcat gatttcataa attttctcca cgccgcgaaa acagaagcca ttagcacagt
     1981 tcagtgttgg gttcgtggag gcaatgtccg gcggagcatt aaaatcagca ctgaagccgt
     2041 aggggtgata gccgacagca tccaaacaac tgccaccgcc cgcattgatt atctctttga
     2101 gaaattcgcg ttcgtcctga taagcgccgt tatggccggc atgaccattc cagctgcccg
     2161 tgactcggcc cgtcggcgcg agtccggccg agacgacgat ggcagtcgga tctgcctgtt
     2221 taattttgtt gtaggctgca cacagcaccg tcttatactt ttgggcatcg ggtgccgcgt
     2281 tccaaccgta attcgcatcc agattcactt cgttgccgag ctcatacgca tcgacgtgat
     2341 ctttgtagac gagggtgtaa tctagatcat tgaggaaggt gcttaactga ttatacgatt
     2401 gtttgttgac ggtaattcga agcaagacat gcgcccaact tggcggcgga tcgatcggcg
     2461 gcacgttgta atacttgatc cagtcgaagc ccatgttgtt aagaagtgtg taatcttgtt
     2521 cggcaacgtt gaaaccatat cccaaatgcg gaatgggcac cgcccgcacg gtcgtaacac
     2581 cgatcagcgc cgctaaaaca atcgggataa gccaaaccgc acgcaaaaaa attttgggac
     2641 ataaagccgg atcgatcttg aacatagcgt tattataaac aatcgatcga catcggccaa
     2701 tgaggaatta gtcattt
//
LOCUS       DASRTJ010000470         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_29626,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000470 DASRTJ010000000
VERSION     DASRTJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29626"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..549
                     /locus_tag="VFF70_09575"
     CDS             61..549
                     /locus_tag="VFF70_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZY44984.1"
                     /translation="MLDDAHTATNWLVQQPSVDVNQLALVGSSIGGYVTLAAGTNDLR
                     FKYLVSICPFVNGQAVPMSIENSIEWARMLNGITSEQLKHEWDHLPPVHAFKDQLIDR
                     NILLISGSQDTLFPISHHQPLVDLLPKIHWTRLTDGDHTFNTCRRQLVNVVIEWLSNQ
                     LN"
     gene            577..1071
                     /locus_tag="VFF70_09580"
     CDS             577..1071
                     /locus_tag="VFF70_09580"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZY44985.1"
                     /translation="MLEAIELSTVLPATPRKLYTAWLSSKEHTAMTGGQASIEPEVGG
                     KHYEWDGYIWGQNLELEPFKRIVQSWHSAEFPEGSQDSRLEVRFEAVKGGTEVTLIHS
                     AIPEGQGESYRDGWDENYFQPMKKYFKPKKTSAPKTVKPRSKPKAANAKKPTGAKTTR
                     RKTR"
     gene            1068..1598
                     /gene="coaD"
                     /locus_tag="VFF70_09585"
     CDS             1068..1598
                     /gene="coaD"
                     /locus_tag="VFF70_09585"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZY44986.1"
                     /translation="MTKAIYPATFDPVHFGHIDIAKRAANIFDEVIIGVYDRPLKSLL
                     FSVEERLRLVQLALKDLKNVHIQRYDGLTVDFAHEVGAQVIVRGLRVVSDFELEWHMA
                     LTNKQLAPDIEVVCLMTSQAFAFLSSSTVREVALLGGNVTSMVPAFVAEALHAKAVEQ
                     ASDPANHPVPMTSLRD"
     gene            1714..2241
                     /locus_tag="VFF70_09590"
     CDS             1714..2241
                     /locus_tag="VFF70_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZY44987.1"
                     /translation="MDILHLIDRLEAILNEGSHPPLIKKVMIDEQRAWEVIDQMRIAI
                     PDEVKKAKRTIQERDRIMAQANEEAARVVELAKEDAAQLTIDHDIAKSAQNRASQIIE
                     RAQREAEQLRTDADDYSLQVLSDLEAELAKQLTVSRNGLLKLQSDRAAREAQAGSEDQ
                     SDSASTASVPSTHRS"
     gene            2317..2535
                     /gene="rpmF"
                     /locus_tag="VFF70_09595"
     CDS             2317..2535
                     /gene="rpmF"
                     /locus_tag="VFF70_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZY44988.1"
                     /translation="MGPLPKRKHSKGRRNRRRAHYKISPMTLQLCPTCKELKLPHRVC
                     PNCGNYDGALVVDVQARAARRTAKKTDQ"
     gene            2607..>3002
                     /locus_tag="VFF70_09600"
     CDS             2607..>3002
                     /locus_tag="VFF70_09600"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZY44989.1"
                     /translation="MTHSIAFLFPGQGSQFVGMGRGLIDQYPEARAIFDQADQILGRS
                     LSRLCLDGPEDQLTDTVNAQPAILVTSIATLGVIAKHAPHIKPAFVAGHSLGEFSALV
                     ATGSLSFEDAVRLVQERGRVMKAAGAAQPG"
BASE COUNT          754 a          797 c          810 g          641 t
ORIGIN      
        1 tgcctgtact ttcattatcg aggctgttgg tgatcagaag gaacttattc gtcaaatggc
       61 ttgttagatg atgcgcatac cgcgaccaat tggctggttc aacaaccatc ggtggatgtg
      121 aatcagcttg cactcgtcgg cagcagcatc ggtggatacg taacattggc tgccggcaca
      181 aatgatctgc gtttcaaata tcttgtatcg atttgtccat tcgtcaatgg gcaagctgtg
      241 ccgatgtcga tcgaaaattc gatcgagtgg gcgaggatgc tcaatggtat caccagcgag
      301 cagctcaagc atgaatggga tcacctcccg ccggttcatg ccttcaaaga tcaactgatc
      361 gatcggaata ttttattgat tagcggcagc caagacactt tgtttccaat cagccaccat
      421 caaccgctcg tcgatcttct gccgaagatt cattggacgc gtttgaccga tggcgaccat
      481 acattcaaca cctgtcgccg gcaactcgtc aacgtcgtca tcgaatggtt gtcaaatcag
      541 ttgaattagt gagataattg aacctggaga atgcccatgc tagaagcgat cgaactctca
      601 actgtcttac ccgcgactcc gagaaaattg tataccgcgt ggctcagcag caaagaacat
      661 accgcgatga ccggcggtca agcctcgatc gagcctgagg ttggcggcaa gcattacgaa
      721 tgggacggtt acatttgggg ccagaacttg gaacttgaac cattcaagcg cattgttcaa
      781 tcttggcact ccgctgagtt tccagaaggc agtcaagatt cgcgcttgga agttcgcttc
      841 gaggccgtca agggcggaac cgaagtgaca ctcattcact cggccattcc tgagggccag
      901 ggtgaatcat accgggacgg ttgggacgaa aactatttcc agccgatgaa aaaatatttc
      961 aagcccaaga aaacgtcggc gcctaaaact gtcaagccac gttctaaacc taaagcggca
     1021 aatgccaaga agccgacagg cgctaagacc acgcggagga agacccgttg accaaggcga
     1081 tctatccggc caccttcgat ccggtccact tcggccacat cgatattgcc aaacgcgccg
     1141 cgaatatttt cgacgaagtg atcatcggag tgtatgatcg gccgttgaaa agcctcctgt
     1201 ttagcgtaga agagcgcctc cgattggtgc aactggcctt gaaggatttg aagaacgtgc
     1261 acatacaacg ctatgacggc ctgaccgtcg atttcgcaca tgaagttggc gcgcaagtta
     1321 tcgttcgcgg gctgcgcgtg gtcagtgatt tcgaattgga atggcatatg gcactcacca
     1381 acaaacaact tgcgccggat attgaagtcg tgtgtttgat gaccagccag gcgtttgcat
     1441 tcttaagttc cagcacggta cgtgaagtgg cattgctcgg tggaaatgta acgagcatgg
     1501 tgccagcatt cgtggccgag gcgctgcacg cgaaggccgt tgaacaggcc agtgatccgg
     1561 cgaatcatcc agtaccgatg acgtcattga gagattgata atcggagggg cgaagtgacc
     1621 tcgccccaac ttgcaatttt ttcaaacttg taagacactg tatccagcct gagtacacca
     1681 gctagcagca tgagcacacc atctggagct gatatggaca ttcttcacct aatcgatcga
     1741 cttgaagcta tcctcaacga gggatcgcat ccgccgttaa tcaagaaagt catgatcgat
     1801 gagcagcgcg cctgggaagt catcgatcaa atgcgcattg cgatccccga tgaggtcaag
     1861 aaagcgaagc gcacgatcca agaacgcgat cggatcatgg cgcaggctaa tgaagaagct
     1921 gcgcgcgtcg tcgagctggc gaaagaagac gcagcgcagc tcacgatcga tcacgatatc
     1981 gccaagtcgg cgcagaatcg ggccagtcaa atcatcgagc gggctcagcg tgaagcggaa
     2041 cagttgcgca ccgatgccga tgattacagc ctgcaagtcc tgtccgacct cgaagcagag
     2101 ttggccaagc agctgactgt gtcgcgcaac ggcttgctga agctgcaatc tgatcgggcg
     2161 gcgcgggagg cgcaagcggg cagcgaagat caatcggatt cagcgtcaac cgccagtgtt
     2221 ccatcaaccc atcgatctta aatcacttgt cttgacaggt cacccgcatc tgctataatc
     2281 gtcgcctgat tcatttgaaa ggatattacc agccagatgg gaccattacc gaagcgaaaa
     2341 cactctaagg gccgccgcaa tcgccgccgg gcgcattaca aaataagccc gatgacgctg
     2401 cagctgtgcc cgacctgcaa agagttgaag ctgccacacc gcgtctgccc gaactgcggc
     2461 aactatgacg gcgcgctcgt cgtggatgtg caggctcgcg ctgcgcggcg cactgcaaaa
     2521 aagaccgacc aatagtcaac cgcaatgact cacacacggg gccgctgtcg atcacacggc
     2581 ctcgtatgtt gtcagggcaa tgaacgatga ctcactcgat cgcgtttctg ttccccggac
     2641 aaggatcgca attcgtcggc atgggccgtg gattaatcga tcaatatcca gaggcgcgtg
     2701 ccatcttcga tcaagccgat cagattttag gccggagctt atctcgattg tgtctggacg
     2761 gccccgaaga tcagttgacg gataccgtca acgcccagcc tgcgattttg gtcacgagca
     2821 ttgcaacatt gggtgtgatt gcgaagcacg cccctcacat caagccggcc tttgttgccg
     2881 gccacagcct cggcgaattc agcgcactgg ttgcaactgg atcactttca tttgaagacg
     2941 cggttcgatt ggttcaagaa cgtggccgag tgatgaaagc ggcgggcgcg gcacagcccg
     3001 gc
//
LOCUS       DASRTJ010000471         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_67572,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000471 DASRTJ010000000
VERSION     DASRTJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67572"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..1170
                     /locus_tag="VFF70_09605"
     CDS             493..1170
                     /locus_tag="VFF70_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZY44990.1"
                     /translation="MSGTITALVAQKKNKDRVNVFLDGQFVFGLAAIEAIRLKRGQVL
                     SDADIDRLRGADEVEKAHHRALNFLAVRPRSISEVRQNLKKAEVSPDVIEQVIDRLQR
                     ADLLNDAEFIKYWLENRSAFSPRSSRALKQELMRKGVDREAIDQVLGEVKHDEEGAAT
                     RSALAKANRFKQLPQKEFALKLSQYLARRGFGYEIIREAVAAAWRSIHAAEAEASIDQ
                     PFDELEE"
     gene            1173..2705
                     /gene="rny"
                     /locus_tag="VFF70_09610"
     CDS             1173..2705
                     /gene="rny"
                     /locus_tag="VFF70_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZY44991.1"
                     /translation="MDIALVILIALAAAGVGGAIGYVVAKNRNKVAKQELDVERARIV
                     EAAQIKAKEVALAAKDEAIKVRDDVETELKRRRGEWQREEERLNKRREELDQKLEKVD
                     QRERKLNQRQSQMDKTQNDLEKEHAQIMIELERSAGMGREEAKQELLKMVEAEARGDM
                     ARVLRQVEAEIKEQADQKARELVVLAVQRLATEQAAEVSTSLVPLPNDEMKGRIIGRN
                     GRNIRAIERATGVDIVVDDTPEAVTLSSFDAVRREIAKIALSKMIQDGRIHPTRIEKL
                     VEDAEAEVERVIKDEGERAAIEAGVPGLHPEIIKLLGRLRFRTSYGQNQHYHAIESAH
                     IAGILAAELGADVKVAKIGGLLHDIGKAIDHEFEGTHAKLGAEFAKRFGINNPKVLNA
                     IAAHHHEVPQESIEAIIVEMSDAISGSRPGARRENVETFLKRVRELEDIATAHKGVAE
                     AYAIQAGREIRVVVRPEDIDDLASIQLSKDIAKKIEETMQYPGQIKVTVLRETRATEY
                     AK"
     gene            complement(2873..4018)
                     /locus_tag="VFF70_09615"
     CDS             complement(2873..4018)
                     /locus_tag="VFF70_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZY44992.1"
                     /translation="MAISQKSLRSNTHLRENRAESLFDLSLPKTFDNRVAHSIAIIAT
                     RLWQTTTAGLVTRYTAHLTVLVVVIAAIALGRPAIAAPAVNNTNKVLTSSVETSSDSS
                     LQETFAEHGLFVDSNIVSRLPNAHTTVPFRTRREIVIYRVQPGDNVQGIAAAFGLQPE
                     TILWSNPAIEDLPDLLKIDQEVVILPIDGVYHEVKTGDTLDGIAKLYKVDVANITDVP
                     WNNLTPPNYDITAGSKLIVKDGTKPFIPKVVTSYSGPVPTGAQGSGQFRWPVVGYITQ
                     DFWAGHKALDISAPTGSPVYASDAGYVSFSGWTDVGYGWLVRINHGNGFETLYAHNSQ
                     LLVVAGEAVARGQLIAYAGSTGHSTGPHCHFEIRLNGVPVNPRLYLP"
     gene            complement(4133..>4252)
                     /locus_tag="VFF70_09620"
     CDS             complement(4133..>4252)
                     /locus_tag="VFF70_09620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY44993.1"
                     /translation="QGTVIAAAGSTGRSSGPHLHFETHFNGAPQNPFNVLPPD"
BASE COUNT         1031 a         1036 c         1305 g          880 t
ORIGIN      
        1 gtttattaaa agaacaaaaa ttttgactgg cacttgcact tatctaatgg tataatcgca
       61 tcaagttatt ttaaggtttc atttgcccat ccatttatcg ggcgagcgcc gcgcaaaacg
      121 aataaccgcg cggcccatag aacgcaccgc cgcagcaacg ttgatacatc ggcggtccgt
      181 tgattcgatt gtaaagatac ggacggctat ccaaccggat agcgctgaga gtccagtggt
      241 ttgtagttgt aggacttcgg caggtgtgcc ggagcagtac agaatgcctg tgtgattggg
      301 tgtagaccat ttagtgagtg tcccgcgcca gcggcatatt ggcatacctg tcagttgagt
      361 taacacaccg tatcttgttg tagttgcaat ccgaatcgcg gacatccgct gtcgatcgac
      421 gagctgtggc tgagtcaagt catggctcgt tttgtttatt gaccgtagac cgcggacggc
      481 ggactgcaaa agatgtcggg aacgattacc gcgctcgtcg cgcagaagaa aaataaagat
      541 cgcgtcaatg tgtttctcga cggccagttt gtttttgggc tggcggcgat cgaggcgatc
      601 cgcctgaagc gcggacaggt gttgagcgat gcggacatcg atcgactgcg cggggccgat
      661 gaagttgaga aggctcatca tcgcgcgctg aactttttag ctgttcggcc gcgcagcatc
      721 agcgaagtcc gccagaactt gaagaaggcc gaagtcagtc ccgatgtgat cgagcaagtc
      781 atcgatcgct tgcagcgcgc ggacttgttg aacgatgcgg agtttatcaa gtactggctg
      841 gaaaaccgat cggccttctc gccccgcagt tcgcgcgcgc tgaaacaaga gttgatgcgc
      901 aagggcgtcg atcgtgaggc gatcgatcaa gtgttgggcg aagttaagca tgacgaagaa
      961 ggcgcggcca cccgatcggc gttagctaag gccaatcggt ttaagcaact gccgcagaaa
     1021 gagttcgcgc tgaagttgag ccaatatctg gcgcggcgcg gcttcggtta cgagatcatt
     1081 cgagaagcag tcgcggccgc gtggcgatcg atccacgcgg ctgaagcgga agcatcaatc
     1141 gatcaaccat ttgatgaatt agaggagtaa tcatggacat tgcattagtc attttgattg
     1201 cgctggccgc ggcgggagtc ggtggggcca ttggctatgt cgtcgccaaa aatcggaaca
     1261 aagtagccaa acaggaattg gatgttgagc gagcgcgcat cgtcgaagcg gcgcagatca
     1321 aagccaagga agtggcttta gcggccaagg atgaagcgat caaagttcgc gacgatgtgg
     1381 aaaccgagct caaacgccgc cggggcgagt ggcagcgcga agaagagcgc ttgaacaaac
     1441 ggcgtgaaga actggatcag aaactggaga aagtcgatca gcgcgagcgc aaattgaatc
     1501 agcgccagag ccagatggat aagacgcaga atgatctgga gaaagagcac gcgcagatca
     1561 tgatcgagtt ggagcgcagc gccgggatgg gccgcgagga ggcgaagcag gaactgttga
     1621 agatggtcga ggccgaggcg cgtggcgata tggcgcgtgt cttgcgacaa gttgaagccg
     1681 agatcaagga gcaggccgat caaaaagcgc gcgagctcgt ggtcttagcc gtgcagcgcc
     1741 tcgcgaccga gcaagcggct gaagtttcaa cgagtctggt gccgctgccg aatgacgaga
     1801 tgaaaggccg tatcatcggc cgcaacggcc gcaatatccg cgcgatcgaa cgtgcaaccg
     1861 gcgttgatat tgtcgtcgac gatacaccgg aagcggtgac gttatcctcc ttcgatgcag
     1921 tgcggcgcga aatagcgaag attgcgctga gcaagatgat tcaagatggg cgcattcatc
     1981 cgacacggat cgagaagctg gtcgaagatg ccgaggccga agtcgagcgt gtgatcaaag
     2041 acgaaggcga acgcgcggcg atcgaagcgg gcgtgccggg cctgcatccg gaaattatca
     2101 agctgctcgg ccgcctgagg tttcgcacgt cctacggaca gaaccagcat tatcacgcca
     2161 ttgagtcagc gcacatcgcg ggcatcctcg ccgctgaatt gggcgccgat gtgaaagtgg
     2221 ccaagatcgg cggcttgctg cacgacatcg gcaaggcgat cgatcatgag tttgaaggca
     2281 cgcatgcgaa actcggcgcc gagtttgcca aacgctttgg gatcaacaat cccaaggtcc
     2341 tcaacgcgat cgcggcgcat caccatgaag tgccgcaaga atcgatcgag gcgatcattg
     2401 tcgaaatgtc ggatgcgatc tccggctcgc ggccgggcgc gcggcgcgag aacgtggaaa
     2461 cgttcttgaa gcgtgtgcgt gagctggaag atattgccac cgcgcataaa ggtgtggccg
     2521 aggcttacgc gattcaagcc ggacgtgaaa ttcgggtggt cgtgcggcct gaagacatcg
     2581 atgatctcgc ttcgatccaa ttgagcaaag atattgccaa gaagatcgaa gagacgatgc
     2641 agtatccagg ccagatcaaa gtgacggtgt tgcgcgaaac tcgagcgacg gagtacgcga
     2701 aataaaggat cagtgatcag ttgattaagg attaggaaaa cagaacagcg ctcgagtttt
     2761 gtgaactcga gcgctgttct gttttagagc aatcggcgat tactcttctt atatgaagta
     2821 accgccgatt ttcttttcct gatcactgat ccccgattaa ctgatcccta tcctacggca
     2881 aatacaatct tggattcacc ggcacgccgt tcagccgaat ctcaaagtgg cagtgcgggc
     2941 cagtcgagtg gccggtgctg ccagcgtagg cgatcaattg tccgcgagcc acggcttcac
     3001 ccgcgacgac gagcagttgg ctattgtgcg cgtacaaagt ttcaaagccg ttgccgtgat
     3061 tgatacgcac cagccaaccg taacccacat ctgtccagcc ggaaaatgac acatagccgg
     3121 catccgaggc atagacaggt gagccggtag gcgcgctgat gtctaatgct ttgtggcctg
     3181 cccagaagtc ttgagtaatg taaccgacga ccggccagcg gaactgtccg ctgccttgcg
     3241 cgcctgtcgg gaccggaccg gagtaactgg tgaccacttt cggaataaag ggcttggtgc
     3301 catctttaac gatcaatttt gagccggcgg tgatatcgta attcgggggc gtcaaattat
     3361 tccacggcac gtcggtgatg ttggcgacat ctactttgta aagcttggcg atgccgtcca
     3421 aggtgtcgcc cgtcttcact tcgtggtaga caccatcgat cggcaggatg acgacctctt
     3481 gatcgatctt cagtaaatcg ggcaagtctt caatcgctgg atttgaccac aagatcgttt
     3541 caggttggag gccaaaggct gctgcgatgc cctgcacatt atcgccgggt tgaacgcgat
     3601 agatcacgat ctcgcgtcgg gtgcggaagg ggacggtggt gtgcgcattg ggtaagcggg
     3661 agacgatatt tgaatcgacg aacaagccgt gctcggcaaa agtttcttgg agtgaggagt
     3721 cgctgctcgt ttctaccgaa ctagtcagga ccttgttagt gttattgacc gccggggccg
     3781 caatcgcggg acgacctaaa gcaatggccg cgataactac caccaagact gtcagatgcg
     3841 cggtataacg agtgactaaa ccggctgtgg tggtctgcca taaacgtgtc gcgatgatgg
     3901 cgatgctatg cgctacacgg ttatcgaacg ttttgggcag agataaatcg aacagcgact
     3961 ctgcgcgatt ttccctgaga tgggtattgg accggagtga cttttgagaa atagccataa
     4021 tagtttaatg aacactgtcg ccggtcgaac aacccggtaa gactgtgagt agttcagccc
     4081 aacagctgtc ttcgcggcga gagtataccc caattggcca gaagtgccag cgtcaatcag
     4141 ggggcaagac gttaaacgga ttttggggag cgccgttgaa gtgcgtttcg aaatggagat
     4201 gcgggccgga ggaacggccg gtcgatccgg cggcagcgat gacagtgcct tg
//
LOCUS       DASRTJ010000472         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_277754,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000472 DASRTJ010000000
VERSION     DASRTJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277754"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="gatB"
                     /locus_tag="VFF70_09625"
     CDS             <1..998
                     /gene="gatB"
                     /locus_tag="VFF70_09625"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZY44994.1"
                     /translation="MEMVTEPDFRSVEEVQAYALKLRAILRYLGVNSGDMEKGVIRFE
                     ANISIRPAGSKEFGTRTEVKNLNSFRALVRATEYELARQQKIVEGGGTIDQATLGWDE
                     ARNITLMQRSKEQAHDYRYFPEPDLPPLVIDPKWVAEIKAALPELPDAKRDRFARDYS
                     LDLNEAELLLAERSVAEYFEAAVKLSGGQKAKSVFNWLTGELFRYMNEAAISIEEVKV
                     TPKMLVELIDLVEAGTINLNTGKRVLGDMFKSGESATSIVAAKGLAQISDNSAIESIV
                     QKVLAANAAEVEKYLGGKENVLGFLVGQIMKESKGKANPNAVQEILKKQLDMKRE"
     gene            complement(1115..2407)
                     /gene="clpX"
                     /locus_tag="VFF70_09630"
     CDS             complement(1115..2407)
                     /gene="clpX"
                     /locus_tag="VFF70_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZY44995.1"
                     /translation="MKHRDHLRLDNEPTHCSFCLRSKEDVNRLIAGPEGVAICDACID
                     LCRDMLGAEALPATPIDFQPDQIPSPRSIYAQLNEYVVGQDRAKRVLSVAVYNHYKRI
                     NSAKSSEVELEKSNILLIGPTGCGKTLLARTLARILDVPFSIADATSLTEAGYVGEDV
                     ENILLRLMQAADFDVTRAEKGIIYIDEIDKIARKSGDNPSITRDVSGEGVQQALLKIV
                     EGTIANVPPQGGRKHPQQDYIQIDTSNILFICGGLFDGLPKIVSDRLGLKSTIGFTAN
                     QAKREMSESDLLQHITPDDMMHFGLIPEFVGRLPVAVSVDPLDKAALIDILTKPRNAL
                     IKQFQRLIGLDHVELVFTDDALNAAAEEALKQETGARGLRTIVERALLDVMYEIPSRQ
                     DIRKCVINADTIRGLTPPILISQSGQAIDWNTPQSKAA"
     gene            complement(2461..3081)
                     /locus_tag="VFF70_09635"
     CDS             complement(2461..3081)
                     /locus_tag="VFF70_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019640204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZY44996.1"
                     /translation="MESPLNLIPMVVQQDARGERAYDIYSLLLKERIIFLGTPINDQV
                     ANVIVAQLLYLNSEDPDKEIRMYINSPGGQITAGLAIYDTMKMIPAPVSTTAVGLAAS
                     FGTILLTAGAKGKRYALPHATIHLHQPLGGVEGQAADIEIEARQILKMRDLLNGILKE
                     STGLSEADISKYTDRNFYMTADEAVKLGIIDSVLRPQELVSANGKK"
     gene            complement(3297..>3562)
                     /locus_tag="VFF70_09640"
     CDS             complement(3297..>3562)
                     /locus_tag="VFF70_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY44997.1"
                     /translation="EELRPTAEIRLKRGLVLSELIKAEGLQIDEAAIDQRIDEMVERY
                     GVRADDARKSLSTEENRDALRLDLLSQAGVDRAAAIAKGEVSN"
BASE COUNT          774 a          906 c         1044 g          838 t
ORIGIN      
        1 tgatggagat ggtcaccgag ccggactttc gatcggtgga ggaagttcaa gcgtacgcgc
       61 tgaaacttcg cgcgatcttg cgctatcttg gcgtgaacag tggcgatatg gagaagggcg
      121 tcattcgctt cgaagcgaac atttcgatcc ggccagcagg ctcaaaggaa tttgggacgc
      181 gcacggaagt gaagaatctg aatagtttcc gcgcgttagt gcgagccacc gagtacgaat
      241 tggcgcgcca gcaaaagatc gttgagggcg gcggcacgat cgatcaagcg acactaggct
      301 gggatgaagc ccgcaatatc acgttgatgc agcgcagcaa agagcaggcg cacgactatc
      361 gatatttccc cgagcccgat ctgccgcctc tcgtgatcga tccgaagtgg gtggctgaga
      421 tcaaagcagc tctacccgaa ttgccggatg ccaagcgcga tcgatttgcg cgcgactaca
      481 gtctcgattt aaatgaggca gagttattgt tagcggaacg atcagtcgcc gagtattttg
      541 aggcggcggt caaactatcg ggtgggcaga aagccaagag cgttttcaat tggctgacag
      601 gcgagttgtt ccgctacatg aatgaagcag cgatttcgat tgaggaagta aaagtcacgc
      661 cgaagatgtt ggttgaattg atcgatctcg tcgaggcggg cacgatcaat ctcaacacgg
      721 gcaagcgcgt gttgggcgat atgttcaagt ccggcgaatc ggccacatcg atcgtagctg
      781 cgaaggggct cgcgcagatt agcgacaaca gcgcgatcga atcgatcgtg cagaaggtgc
      841 tggcggcgaa tgcggctgaa gtcgaaaagt atttgggcgg caaagaaaat gtactgggct
      901 tcctcgtcgg ccagatcatg aaagagagca aaggcaaggc caatccgaat gcggtgcaag
      961 agattttaaa gaagcagttg gatatgaaaa gagagtaatc agggattggg gattagtagg
     1021 ggcgagcggc agctcgccct ttttgttaat tgatctacag caatatgaga ttgcttcgtc
     1081 ggcccaacgg cctcctcgca atgacgcgcg agatttacgc tgctttactt tgcggtgtgt
     1141 tccagtcgat ggcttgcccg gattgcgaga tcagtatcgg cggagtgagg ccgcggatgg
     1201 tatccgcgtt gatgacacat ttgcggatgt cctgccgcga gggaatttcg tacatcacgt
     1261 cgagcagcgc gcgttcgacg atcgttctca agccgcgcgc gccggtttcc tgtttcagcg
     1321 cttcttcggc ggcggcgttg agcgcgtcat cggtgaagac gagttcgacg tgatctaagc
     1381 cgatcaggcg ctggaattgc ttaatcagcg cattgcgcgg cttggtgagt atatcgatca
     1441 gcgcagcctt gtccaacgga tcgacgctga cggcgaccgg caagcggccg acaaattcgg
     1501 ggatcagtcc aaagtgcatc atatcgtcgg gtgtgatgtg ctgcaggaga tcggattcgc
     1561 tcatctcgcg cttggcttga ttcgccgtga agccgatcgt acttttcaag cccaatcgat
     1621 ccgagacgat cttcggcagg ccgtcgaaca gtccgccgca gatgaagagg atattggatg
     1681 tgtcgatctg gatgtaatct tgctgcggat gcttgcgtcc gccctgcggc ggcacattcg
     1741 cgatcgtgcc ttcgacaatc ttgagcagcg cttgctgcac gccttcaccc gatacatcgc
     1801 gcgtgatcga tggattgtcg ccgctcttgc gcgcgatctt gtcgatctcg tcgatgtaga
     1861 tgatgccttt ctcggcgcgg gtcacatcga agtcggcggc ctgcatcaag cgtagcaaaa
     1921 tgttttcaac atcttcgccg acgtaaccgg cttccgtcag cgaagttgca tccgcgatcg
     1981 aaaacggcac atcgagtatg cgtgccaagg tgcgagctaa cagggttttg ccgcagcccg
     2041 tggggccgat gagcaatatg ttgctctttt cgagttcaac ctcggaactt ttggccgagt
     2101 tgatgcgctt atagtgattg taaaccgcga cgctgaggac gcgcttggcg cgatcctgtc
     2161 cgacgacata ttcgttgagc tgtgcgtaga tcgatcgggg cgagggaatc tgatcgggtt
     2221 ggaaatcgat cggagtagcc ggcagcgctt ctgcgcccaa catatcgcgg cacagatcga
     2281 tgcacgcatc gcaaattgcc acaccctccg gcccggcgat caatcgattg acatcttctt
     2341 tgctgcgcag gcaaaaagag cagtgcgtgg gttcgttgtc caggcgaaga tgatcgcgat
     2401 gtttcatgtt cttcgagtta aaaattcgct ccatgtttgt ggcacggggc ggacgcgatt
     2461 ttacttcttg ccgttggcgg acacaagttc ctgcggccgc aaaacggaat cgatgatgcc
     2521 gagcttcacg gcttcgtcgg cggtcatgta gaagttgcga tcggtgtact tgctgatgtc
     2581 ggcttcactc aggccggtcg attctttgag aatgccgtta agcagatcgc gcattttcaa
     2641 aatctgccgc gcttcgattt cgatgtcggc agcttggcct tctacgccac cgagcggctg
     2701 atgtaaatga atggtggcat gcggcagagc gtagcgtttg cctttggctc cggcagttaa
     2761 taggatcgtc ccgaagctgg cggccagccc gacggcggtc gtcgatacgg gcgcagggat
     2821 cattttcatc gtgtcataga tcgcgagacc tgcggtgatt tgtccgcccg gcgagttgat
     2881 gtacatccga atttctttgt ctgggtcctc gctgtttaag tacagcagct gcgcgacgat
     2941 cacattggcg acttgatcat tgatcggcgt cccgaggaag atgatgcgct ctttgagcag
     3001 cagcgagtag atgtcatacg cgcgctcgcc gcgggcatcc tgctgtacaa ccatcgggat
     3061 caaattcagt ggtgattcca tttgcgactc cttgtaagat gagtagaaca tcgtgctgtc
     3121 gattatatct gttcgatgtt cactctgaac tgatcgaatc tacaggttgc gatcgatcta
     3181 atagaactct aacacatttt cgaattaact atgcttaacg cgcaattctc aatgttgact
     3241 ccaacgcctc ggccgttttt tcggctcgat atgccgcaga aaaagaagaa gcaggtttag
     3301 ttagacactt cacctttggc aatcgctgca gcccgatcga cacccgcctg gctcaaaaga
     3361 tcgagccgga gcgcatcgcg attttcctcg gtcgataacg acttacgcgc gtcgtcggca
     3421 cgcacgccat acctttcaac catctcgtca atccgctggt cgattgccgc ctcatcgatc
     3481 tgaagacctt cggccttgat caattcgctc aacaccagac cgcgcttcaa tcgaatctcg
     3541 gcggtgggcc ggagttcttc gt
//
LOCUS       DASRTJ010000473         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_332667,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000473 DASRTJ010000000
VERSION     DASRTJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332667"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..>1510)
                     /locus_tag="VFF70_09645"
     CDS             complement(121..>1510)
                     /locus_tag="VFF70_09645"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZY44998.1"
                     /translation="QADDDKLAKSMTADGKFTPSQKMRLQKLGIKGETLADLSTEDKR
                     RMFRLDIDPASITWQRVVDINDRMLRKIQIGLGDEEKGNERFTGYDITVASEIMAILA
                     LTTGIDDMRRQLGAMVFGTNTRGEALTAEDLGVAGALTVLMKDAIMPNLMQTLEGTPA
                     FVHAGPFANIAHGNSSIVADQIALKLVGKDGYVITESGFGADIGMEKFMDIKCRYSGL
                     IPDVVVIVATIRALKMHGGGPKVVAGRPLDPAYTSENLALLEKGMPNMVRHIHNAKKF
                     GVPVVVAVNSFKDDTDREIDLVRKFAIEAGAEGAYKCTHWMHGGLGAIDLAKAVMAAA
                     KLPKKFKFLYPLELSIKDKIDAIAKFYGAAGVDYSPEAEAKIDLYTRLGFDKLPICMA
                     KTHLSFTDKPEIKNAPTGFRLPIRDIRASVGAGFLYPLVGSMRTMPGLPTRPVFFDVD
                     LDLKTGKVLGLF"
BASE COUNT          296 a          454 c          416 g          344 t
ORIGIN      
        1 ctgacgcgcg cgaaataaaa cctcaaatat ttcctgatca aaaaaactcc cgaagacact
       61 acagccctcg ggagtttttc ttttgtctgc gtaatccgta aaatccgcgt ccggcttatt
      121 ttagaacaag cctaacacct tgcccgtctt cagatcgagg tcgacatcga agaacactgg
      181 ccgagtcggc aggccgggca tggtgcgcat cgatccgacg agcggatata agaagcccgc
      241 gcccacactt gcgcgaatgt cgcggatggg caggcggaaa ccggtcgggg cattcttgat
      301 ctccggttta tcggtgaaag acaaatgcgt cttcgccatg cagatcggca gtttatcgaa
      361 accaagccgc gtatacagat cgatcttcgc ttcggcttcc ggcgagtagt ccacgccagc
      421 cgcgccatag aacttggcaa tcgcatcgat cttgtctttg atcgaaagtt cgagcggata
      481 caggaacttg aatttcttcg gcagcttggc cgcggccatg accgccttcg ccagatcgat
      541 tgcgccgagg ccgccgtgca tccagtgcgt gcacttgtac gcgccttccg cgcccgcttc
      601 gatcgcaaac ttgcggacga gatcaatttc ccgatcggtg tcgtccttga acgagttcac
      661 cgccacgacg accggcacgc cgaatttctt ggcattgtgg atgtggcgca ccatattggg
      721 catgcctttt tcaagcagtg ccaggttttc gctggtgtac gccggatcga gcggacgacc
      781 ggccaccacc ttcggcccgc cgccgtgcat cttcagcgcg cggatcgttg cgacgatcac
      841 cactacatcc gggatcaaac ccgaatagcg gcatttgatg tccatgaatt tttccatgcc
      901 gatgtccgcg ccgaagcccg attcggtgat gacgtagcca tctttgccca cgagtttcaa
      961 tgcgatctga tcggccacga tcgagctgtt gccgtgcgcg atgttggcga acggcccggc
     1021 atgcacgaac gccggcgtgc cttccagggt ctgcatcagg ttcggcatga tcgcgtcttt
     1081 catcaagacc gttaacgcgc ccgccacgcc caaatcttcc gcggtcagtg cctcgccgcg
     1141 cgtgttggtg ccgaagacca tcgcgcccaa ctgacgccgc atatcatcga tgccggtggt
     1201 cagcgcgagg attgccatga tctccgaagc gacggtgatg tcgtaaccgg tgaaacgctc
     1261 gttgcctttc tcttcatcgc ccaagccgat ctgaatcttg cgcagcatgc gatcgttgat
     1321 gtctaccacg cgttgccagg tgatgctggc cggatcgatg tccaggcgga acatgcggcg
     1381 cttatcttcg gtcgagagat cagccagcgt ttcgcctttg atgccgagct tctgcaagcg
     1441 catcttttgc gatggcgtga acttgccatc ggcggtcatg cttttggcga gtttatcgtc
     1501 gtcggcctgc
//
LOCUS       DASRTJ010000474         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_29805,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000474 DASRTJ010000000
VERSION     DASRTJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29805"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFF70_09650"
     CDS             complement(<1..331)
                     /locus_tag="VFF70_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY44999.1"
                     /translation="MTPADPREPRYRILVVDDEPRMIRFIQMNLELEGYIVVEAANGF
                     EALNKVRDELPDLIILDVMLPDMDGFETLRLLREISQVPVIMLTVKADEADKVKGLEL
                     GADDYVTK"
     gene            complement(399..644)
                     /locus_tag="VFF70_09655"
     CDS             complement(399..644)
                     /locus_tag="VFF70_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45000.1"
                     /translation="MSITVLVHLVGEEAIMCEADRLPNPDDTNITVMNVRRRDGKDVN
                     FLDASVHSVILPLARINFIEVMQTGEDEEKVVSFVRD"
     gene            complement(666..1133)
                     /locus_tag="VFF70_09660"
     CDS             complement(666..1133)
                     /locus_tag="VFF70_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY45001.1"
                     /translation="MLTGGPLRTEPLPETELPATRADPWQSGLEPSPSGDLLTEITLA
                     IVAPSGRQLVLPIDGEAVLGRLDATRGVFPNIDLTPEGGLEGGVSRRHARIHRQRAQF
                     FIEDLGSANGTFLNGQRLTPYLPHPLHDGDELQLGRVKVRLSLTKSSKQKVSD"
     gene            complement(1202..2689)
                     /locus_tag="VFF70_09665"
     CDS             complement(1202..2689)
                     /locus_tag="VFF70_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZY45002.1"
                     /translation="MNDPLDDLYARLNLSAKATPEEIRHAYRQLARQHHPDTQAAPAG
                     TTLLFRQIQEAYDLLSDPVRRAAYDQQQIEAGRSPDALFHWNMQLSRSILPLIEGEQV
                     IYVLLEIAAGQPVKSIERLPLNLCLVLDRSTSMQGARLDQTKAAAQRMIESLTSEDSL
                     GIVTFSDRAEVVWPNQTLTDPIRTKAKISAIQASGGTEILQGLNAGLVELDKHRRERS
                     INHLILLTDGQTYGDEEQCITAALEAKKRRIGISALGIGEDWNDTLLDAIATRSGGVS
                     QYIASTGEIQHFLRERLQGLGSTYSENLRLTIKATKGTLLQNAFTLLPYIRRIESEKD
                     TLLLGALELNKPITALLEFVIGPHASGLQRVAQLELSGDIPSQNRLGERLRRDVSFTF
                     SPMAAANLAVPPNILSALAKITIFRMQEGAWQALDKGDIVGATNRLETMATRLLDLGE
                     HDLARAALLEAGRLARNGNTSAAGRKAIKYGTRSLTKSLKKEQKS"
     gene            complement(2696..3586)
                     /locus_tag="VFF70_09670"
     CDS             complement(2696..3586)
                     /locus_tag="VFF70_09670"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZY45003.1"
                     /translation="MKLFGKSKKTEMSQAAAAPAAEDTPTTLGRQPVARISQRSGLRV
                     GRATDVGRVRAHNEDSSLTFESTHNGDESLAPLGLFILADGMGGHQAGEIASSLAVRI
                     VANSIIRDVYLPYLMKGTPSAVETPLADALHGAVETANMAVHNQVPGSGTTLTCALII
                     GSRAYMAHVGDSRAYLYHDQQLKQVTHDHSYVDKLVEMGQLTPEAAAVHPQRNVLYRA
                     VGQGDQLEIDIQMIDLPTSSRMLLCCDGLWGMLTDAIIAKILAEAPTPQEACNQLVVA
                     ANEAGGKDNITAVIIESLSA"
     gene            complement(3622..>3975)
                     /locus_tag="VFF70_09675"
     CDS             complement(3622..>3975)
                     /locus_tag="VFF70_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZY45004.1"
                     /translation="WVIWRYRTEKPVVSSPIIGQNVVYIGSADGNLYALEVKSGKLLW
                     KYTTEGQVNSSPAIHHEAVYVGSVDGSVYSLDTKTGNLRWRLRTGGPVISSPVVGADV
                     VYIGSNDHILYALPT"
BASE COUNT          857 a         1046 c         1073 g          999 t
ORIGIN      
        1 gtttggtcac gtaatcatca gcgccgagtt ccagcccttt tactttatcg gcttcatccg
       61 ctttgactgt cagcatgatc accggcactt gcgaaatttc tctgagtaag cgcagcgttt
      121 caaagccatc catgtctggt agcatgacat ccaagataat taaatcgggc agctcatcgc
      181 ggactttgtt gagcgcctca aagccattgg cggcttcaac gacgatatac ccttctagtt
      241 ccaagttcat ttgaatgaac cggatcatgc gcggctcgtc gtcgacaacg aggatgcgat
      301 agcggggttc gcgaggatca gcaggggtca tagtttgatc gaaaaacttg cgctgtttct
      361 gtcaatctcg atcgattttc agaacctgcg caaggttgtt agtcgcggac aaagctaacg
      421 actttttctt cgtcttcgcc tgtttgcatg acttcgatga agttaatgcg cgctaaaggc
      481 agtatgaccg aatggacaga ggcgtccaag aagttgacat ctttaccgtc gcgccgccgg
      541 acgttcatca cggtgatgtt agtgtcatct gggttaggca accgatcggc ttcacacatg
      601 atcgcttctt cgccgacgag atgaaccagg acagtgattg acaaggtcgc tcgcttcgat
      661 ccgatttaat cggacacttt ttgtttgcta gacttggtga ggcttagacg aactttgacg
      721 cgtccgagtt ggagttcatc gccatcatgt aagggatgcg gcagatacgg cgttaagcgt
      781 tggccgttaa ggaaggtacc atttgcgctg cccaagtctt caatgaaaaa ttgcgcccgc
      841 tgtcgatgaa tgcgcgcatg ccggcgtgac acgccgcctt ctagtccgcc ttctggcgtc
      901 aggtcaatat tagggaacac accacgggtg gcatctaacc ggccgagcac cgcttcacca
      961 tctataggca aaacgagttg gcgtccgctc ggcgcgacga tggcgagcgt gatctcagtt
     1021 agaagatcac cggacggcga tggttccaat ccgctctgcc acggatcggc gcgcgtggcg
     1081 ggtagttccg tttcgggcag cggttcggtc cgcagtgggc cgcccgtgag caaatagact
     1141 ccgcattcac tgcagaataa ggttccaggc cgatgtttgg ctccacatga aggacattcg
     1201 atcatgattt ttgttccttc tttaaagact tcgtcaaact acgcgtccca tatttaattg
     1261 ctttgcggcc ggccgccgag gtattaccat tgcgcgctag tcggcctgcc tctaataaag
     1321 cggcacgtgc cagatcatgt tcgcccaaat caagcaagcg tgtcgccatg gtctccaagc
     1381 gattggttgc accaacgata tcacctttgt ctagagcttg ccacgcgcct tcctgcattc
     1441 ggaagattgt aattttggcg agtgcgctga gaatgtttgg cgggacggcc aagttggccg
     1501 ctgccattgg gctaaatgta aacgacacat ctcgtcgtaa ccgctcgccc agtctgtttt
     1561 gtgatggaat atcgccgctc aattcgagct gtgcaacgcg ctgcaagcct gacgcatgag
     1621 ggccgatcac aaattccagc agtgcagtga tgggcttgtt aagttccaat gcacccagta
     1681 gaagcgtatc tttttctgat tcaatgcgcc gaatgtaagg caacaatgtg aatgcattct
     1741 gcaggagtgt gcctttagtc gctttgatgg tcagccgcag gttttcactg taagtggaac
     1801 ccagtccttg aagacgttcg cgcagaaaat gttgaatttc gccggtcgac gcgatgtatt
     1861 gtgacacccc tccactgcgc gttgcaatgg catccagcag agtgtcattc caatcttcgc
     1921 cgatgcccag cgcactgatg ccgatccgcc gcttcttagc ttccagcgcg gctgtgatgc
     1981 actgctcctc gtcgccatag gtctgaccat cggttagtaa gatcaaatga ttgattgatc
     2041 gttcgcggcg atgcttgtct aattcgacca ggccggcgtt caagccttgc aagatctcag
     2101 tgccgcccga agcttgaatg gcgctgattt tggccttagt ccgaattgga tccgtcaggg
     2161 tttgattggg ccagacaacc tcagcccgat cactaaaggt tacgatacca agactgtctt
     2221 cacttgtcaa gctttcgatc atccgctgag ccgcagcttt tgtttgatcg agccgcgcgc
     2281 cttgcatgga tgtgctgcga tcgagcacca gacacaaatt tagagggaga cgctcgatcg
     2341 atttgaccgg ttgcccggcg gcgatttcga gcagcacata aatgacttgt tcgccttcaa
     2401 tcagcggtaa aatcgatcgg ctgagctgca tgttccaatg aaagagtgca tccggcgaac
     2461 ggccggcttc gatctgctgt tgatcgtacg cagcgcggcg gacgggatcg ctcaacagat
     2521 cgtaggcttc ttgaatttgt cgaaagagga gtgtggtgcc ggccggcgcg gcttgagtat
     2581 ctggatggtg ttgtcgtgct aactgtcgat aggcatgtcg aatctcttcg ggcgtggctt
     2641 tggccgaaag gttgagccgt gcgtaaagat cgtccaacgg atcgttcatg gtctcttaag
     2701 cggacagcga ttcaatgata accgcagtga tgttgtcttt gccgccagct tcgttagccg
     2761 cgacgaccag ttgattgcag gcttcttgcg gtgtgggggc ctcggctagt atttttgcaa
     2821 taatcgcatc cgttagcatc ccccagagcc catcgcagca taacagcatg cggctgctgg
     2881 ttggcaaatc gatcatctga atatcaatct caagctgatc gccttgtcca acagctcgat
     2941 acagcacatt gcgctgcgga tgaaccgcgg ccgcttccgg cgtaagctgg cccatctcga
     3001 ccagcttgtc gacatacgaa tgatcgtgtg taacttgttt gagctgctga tcgtgataca
     3061 agtaggcgcg gctatcgccg acgtgggcca tgtaggcgcg tgacccgatg atcaaggcac
     3121 aggtgagtgt cgtaccgctg cccggcactt gattgtggac agccatgttg gcggtttcga
     3181 ctgcgccatg cagcgcatca gccaagggcg tctcaacagc tgaaggagtg cccttcataa
     3241 gatacggtag atagacatct cgaatgattg agttcgcgac gattcgcacg gctagcgaac
     3301 tggctatttc gccggcttga tgtccaccca taccgtctgc cagtatgaac aagcctagcg
     3361 gtgcgagtga ttcatcgccg ttatgcgtgg actcaaaggt tagtgagctg tcttcattat
     3421 gcgcccgcac ccggccgaca tcggtcgcgc ggccaacacg caatccggat cgttgactga
     3481 tccgcgcaac tggttgccgg cctaatgtgg ttggtgtgtc ctcagcggct ggagctgcag
     3541 ctgcttgtga catttccgtt ttttttgatt taccgaacag cttcattcaa aaaccgtcct
     3601 gtctcttaag ctgttgcccg gtcaagtcgg taaagcatac aagatgtgat cgttcgatcc
     3661 gatataaacc acgtctgcac caacgacggg cgacgaaatg accgggccgc ccgtgcgtag
     3721 tcgccagcgc agattgccgg ttttagtatc caggctgtag acactgccat ctactgagcc
     3781 gacatacact gcctcgtggt gaatcgcggg cgaagaattc acttgtcctt cggtggtgta
     3841 cttccagagt aattttccag attttacttc cagggcatat aaatttccgt cagctgagcc
     3901 gatgtacact acattctgtc caatgattgg cgatgaaaca accggcttct cagtgcgata
     3961 gcgccaaatc accca
//
LOCUS       DASRTJ010000475         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_104968,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000475 DASRTJ010000000
VERSION     DASRTJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104968"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFF70_09680"
     CDS             <1..589
                     /locus_tag="VFF70_09680"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZY45005.1"
                     /translation="QRGERTVSVEGPGTTTYVELAYHAPAANDPDFHALITLDAILAG
                     ASSLGGGGTSNASSRLYKALVDTQLAADVGSSIIPTVDPFLFGLSATVRNGHTAAEVI
                     AALDGEIDRLTNEPVKAAEVHKAIKQAKAQFAFGSESVTNQGFWYGWSEVFADYTWFE
                     NYLDRLAAVTIEDVQRVAQKYLRRSNRTVGTYLPK"
     gene            673..1953
                     /locus_tag="VFF70_09685"
     CDS             673..1953
                     /locus_tag="VFF70_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZY45006.1"
                     /translation="MPLDTESTPGPHNIIRKEYPNGMTVLVKENFSSPSVVVDGLFRA
                     NSAADLTGKEGLSSFTTDVLMRGTTHRSFAQIYEEIESVGASVDVSSGINISSFGAKS
                     LAEDLPLVVDILSDVLREPIFPADEIEKVRGEISTSLEERDNDTRRKAGLAFRELLYP
                     KGHPYSRSTEGYRDSVQSITRDDIANFYRSRYGAKGMMVSIVGAVNAADAFKIWENKF
                     GDWIGATPDRVELPGAPRLTDKREKRIDIPGKSQSDLVMGFIGPARSAPDYLAARLGN
                     SILGVFGLYGRIGEQVREKGGMAYYSYSQIEGGVGPGAWRVVAGVSPQNVDKAVKLIR
                     AEIKRFCETKVTARELSDNKSHAIGSMPLGLETNDGVAGVILDMEFYSLGLDYLIRYP
                     DLIQSISAAQIQAAAQQYLDADHFALAVAGPTGI"
     gene            2080..2460
                     /gene="acpS"
                     /locus_tag="VFF70_09690"
     CDS             2080..2460
                     /gene="acpS"
                     /locus_tag="VFF70_09690"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZY45007.1"
                     /translation="MLSTGVDILSVSRVETALKKFGDRFLQRCFTPMEIEMSRGRHSE
                     YAARYAAKEAVSKALGVGLRLMARNGIHFHDVEILNDRFGKPYVVLHNWAKDRAEELG
                     LTEWSVSLTHEREYAIAFVVAMGG"
     gene            2539..3306
                     /gene="arsM"
                     /locus_tag="VFF70_09695"
     CDS             2539..3306
                     /gene="arsM"
                     /locus_tag="VFF70_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HZY45008.1"
                     /translation="MDDITIKTEVRKHYADIALQPKSCCGSDCCGGDDAINQLIDYGG
                     LDAEIVEGANLGLGCGLPTVNAGIRSGDTVLDLGSGAGIDVFLSAKAVGKEGHVIGVD
                     MTPEMLDRARANARKGNYTNVEFRLGEIEHLPVADNTVDVVISNCVINLVPDKRIVYD
                     EIHRVLRSPDAITGNTGGHFSISDVVTYGEMPESIRNDVALWTGCIAGALDRDEYLKI
                     IHDAGFVDVVINKFEVYDYLKGDDYGASSITLEGRKA"
BASE COUNT          810 a          889 c          981 g          759 t
ORIGIN      
        1 ccagcgtggc gagcgcactg tcagcgtgga aggccccggc acaacgacgt atgtcgaact
       61 cgcgtatcac gcgcccgccg ccaacgatcc ggactttcac gcgctaatca cgctcgatgc
      121 gattttggcc ggtgcttctt cgttgggtgg cggcggtaca agtaatgctt catcgcgttt
      181 gtataaagcg ttagtcgata cacagctcgc ggcagatgtt ggatcaagta tcatcccgac
      241 cgtcgatccg ttcctgttcg gcttgagcgc gaccgtgcgc aatggccata cggcggcaga
      301 ggtgatcgcc gcgttggatg gcgagatcga tcgactgacg aacgagccgg taaaagccgc
      361 agaagtgcac aaagcgatca aacaagccaa agcgcaattc gcctttggga gtgaatcggt
      421 cacgaatcaa ggtttctggt acggctggag cgaagtcttc gcggattaca cctggttcga
      481 gaactatctc gatcgcttgg ccgccgtgac gatcgaagac gtgcagcgcg tggcgcaaaa
      541 atatttgagg cgcagtaatc gaacggttgg aacttatctt ccgaagtgat tagtgatcag
      601 ttgatcagta atcagttaat caggaaacaa agacctgatc actgctcaac tgattaactt
      661 tgaaaggaca tcatgccttt agacacagaa tcgactcccg gcccgcacaa catcatccgc
      721 aaagaatatc ccaatggtat gaccgtgttg gtcaaagaaa atttttccag cccgtctgtt
      781 gtcgtcgatg gcttgttccg cgcgaacagc gctgctgatc taacgggcaa agaaggtctg
      841 agctcattta cgacggacgt gctcatgcgc ggcacgaccc atcgatcgtt cgcgcaaatc
      901 tatgaagaga tcgaatcggt cggcgcgtcg gtcgatgtca gttccggcat caacatctca
      961 tcgttcggcg caaagtcgct ggccgaagat ctgccgttgg tcgtcgacat tctatcggat
     1021 gtgctacgcg aacccatctt tcccgccgac gaaatcgaaa aagtgcgcgg cgagatcagc
     1081 acttcgctgg aagagcgcga caatgatacg cgccgcaagg ccgggctggc tttccgcgaa
     1141 ttgctgtatc caaaaggtca tccgtattcg cgcagcaccg aaggttaccg cgacagcgtg
     1201 caatcgatca cgcgggatga catcgccaac ttctatcgat cgcgttacgg cgcaaagggc
     1261 atgatggtct cgatcgttgg tgcagttaac gccgctgatg cattcaaaat ttgggaaaac
     1321 aaatttggcg attggatcgg tgctactccc gatcgagttg agctgcccgg cgcgccgcgc
     1381 ttgacggaca aacgcgagaa gcgcatcgat attccgggca agagtcaaag cgatctcgtg
     1441 atgggcttca tcggccccgc acgatcggca ccggattatc tcgccgcacg tttgggtaat
     1501 tcgatcttgg gcgtcttcgg tttatatggc cgcatcggcg aacaggtgcg cgaaaaaggc
     1561 ggcatggctt attatagcta cagccagatc gaaggtggcg ttggccccgg cgcgtggcgc
     1621 gttgtcgccg gtgtctcccc gcagaatgtc gataaggctg tcaaattgat ccgcgctgaa
     1681 atcaaacgct tctgcgaaac aaaagtcacc gcgcgcgaat taagcgacaa taaatcgcac
     1741 gctatcggat cgatgccgct gggcctggaa acaaatgatg gtgtggcagg tgtcatcctt
     1801 gatatggaat tttatagttt ggggctggat tatttgatcc gctaccccga tctgatccaa
     1861 tcgatcagcg cggcgcagat tcaagccgct gcacagcagt atctcgatgc ggatcatttt
     1921 gcattggcag ttgcggggcc aacgggaatc taaagactgt catttcgagc gcagcgagaa
     1981 atctctatga taatgagctg agatttctcg ccgaaagaac gcggctcgaa attacaccaa
     2041 gattttgcta gatttgtcat tctgcattga tcattcatca tgttatcaac cggcgttgac
     2101 atcctctctg tctcgcgcgt cgaaaccgcg ctcaaaaagt tcggcgatcg atttttgcag
     2161 cgttgcttca caccgatgga aatcgaaatg agccgcggcc gtcactcgga atatgcggcg
     2221 cgttacgcgg cgaaggaagc cgttagtaaa gcactgggcg ttggactgcg cttgatggca
     2281 cgcaacggca ttcactttca cgacgttgaa atcttgaacg atcgcttcgg caaaccctat
     2341 gtcgttctac acaactgggc gaaagatcgc gcggaagaat tgggcttaac cgaatggtcg
     2401 gtgagcctga cccatgagcg cgaatacgcg atcgcgtttg tggtggcgat gggcggctga
     2461 tggataagcc gcctttttat ttgcgtcctt tattcggcac gtccgtctat ataagcaaat
     2521 ctcaagagga gtttcagcat ggacgacatc actatcaaaa ccgaagtgcg caagcattat
     2581 gccgatattg cgctccaacc caagagctgc tgcggctctg attgctgcgg cggcgacgac
     2641 gcgatcaatc agttgatcga ttacggcggc ttggatgcag aaattgttga aggcgcgaac
     2701 ttggggctgg gctgcggcct cccgactgtg aatgccggaa tccgatccgg cgacaccgtg
     2761 ctcgatctgg gcagcggcgc aggcatcgat gtatttttat cggcgaaagc tgttgggaag
     2821 gaagggcacg tcatcggtgt ggacatgacg cccgagatgc tcgatcgagc gcgggcgaat
     2881 gcgcgcaaag gcaactacac caacgtcgaa tttcgattgg gcgagatcga gcatctgccc
     2941 gtggccgaca acaccgtcga tgtcgtgatc tcaaactgtg taatcaacct cgtgccagat
     3001 aaacgcatcg tgtacgacga gattcatcgc gtattgcgaa gtcccgatgc tattacggga
     3061 aatacaggcg gacacttttc catatcggat gtggtgacct acggcgagat gccggaatcg
     3121 atacgcaatg atgtggcttt gtggacgggc tgcatcgcgg gcgcgctgga tcgcgacgag
     3181 tatttgaaga tcattcacga cgccggtttc gtcgatgtgg tcatcaacaa gttcgaggtg
     3241 tatgattatc tcaaaggcga tgactacggc gcgtccagca tcacactgga aggccgcaaa
     3301 gcataatttg ccaacctaca ccgtcattgc gaggagcgtc ctgagcgaag cgccatctgt
     3361 tcgccgcgaa gtcgaaggat gcgacgaagc aatcttgccc ggacgcgagt gggacaccgc
     3421 actgcacgca gtggatgca
//
LOCUS       DASRTJ010000476         9409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_29830,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000476 DASRTJ010000000
VERSION     DASRTJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29830"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFF70_09700"
     CDS             complement(<1..985)
                     /locus_tag="VFF70_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZY45009.1"
                     /translation="MSHYRSSYRTPRPNPTGWITLLIIVIAACALCWPLTSGVFNSIS
                     NVVKGIGSGINNVVQGPQPTLTIAVSPEKGDLFKQLVNTFNSKIVKASTGETLVVNIV
                     QLDPESMLDAAIAGNFQAMSPDSSIWLDQLDRDYEATTQSATGLVGQSVRYAVSPVVI
                     AMWRDKAQALGWPGTPIGWSDILKIATSDPNFKWSHPSTASASGLLATLAEFYAGANK
                     TRGLTADDVQSPNVLQYVTALEKTVRYYGEGEYAIAQRVVQNGRSYLDAFVASEQMLI
                     WANQHGTDLVAVYPSEGSLWQDHPLALLEADKLTDLNRETFRAFAAYATSAD"
     gene            complement(1147..2334)
                     /locus_tag="VFF70_09705"
     CDS             complement(1147..2334)
                     /locus_tag="VFF70_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45010.1"
                     /translation="MNRTRWIFFGVIGVAIAIIAISLIARSVNSNPVGPAAPSGPLEV
                     RIVVALPIEPWVTAAANQFNTENHQLDNNKIHVTIIPMDGLTALSRYDSAAFDPVPTA
                     WIPDSRYLVELANAAYKDRLGRDVFLTDGEYRAKPIALSLFAWGIYGSRARVLETKFN
                     EINWLTLHDAAIASGGWPELGGEPAWGFFKLVVPNPRKNVGGLAAMVAAAGEYYNRPD
                     ISVADVTKPDFQKWLKELMSSVTDISGASAYTAEDFALLGYSVGDGGQMLESDLLANM
                     AGISNRWKDPLVVRYPKFVTWFDFPFSVWIGPETSAQEKNAALEFEKYLLSAPVQQKA
                     VSYGLRPVNADVTVDQPDSPFTKWSAAGVVDVVQRTSAMRSPDRDVLQALLRWFDLNV
                     AVR"
     gene            complement(2471..3148)
                     /locus_tag="VFF70_09710"
     CDS             complement(2471..3148)
                     /locus_tag="VFF70_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZY45011.1"
                     /translation="MASLLEKVKTLISANLHAMVDSALKANSVAVMDEYIRQAENNLE
                     ALEDAAATVGGEVKTLKRKYDDFSAQAAKLDKDIDALLQQGREDLATAAQSKLNSIQH
                     LVESYKEQYDRQQVEYQNLMNAKLKLEAKLTTTKQEREELLALLDLAKSKELTVKTIK
                     SLDDLAGLGDADVTRMADSIRARLDKASAKSEMLASRLDDQMDEVLGKQELDNQLAER
                     KKRLGIN"
     gene            complement(3422..4765)
                     /locus_tag="VFF70_09715"
     CDS             complement(3422..4765)
                     /locus_tag="VFF70_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZY45012.1"
                     /translation="MSDSLFLRRNDLDQLKSTLTDGACCSIIGLSNVGKSTLLRAAAE
                     PDSKSNRSFTVYVDCNLMLALSDQAFYEVTLRSALDALKHLPNVSAELIAAIEKLYRQ
                     VIDADRPGGIIAPLSFNEGIALLCEKLNRRVALLFDEFDDPFGALDGRVFLNLRALHD
                     KYESLVYVTATGAPLADRRHDAEASEFCELFVGQQMILGMPADEIARRAVKAWGKEDG
                     AELDRDEVDFIVAQAGGHPGLLQSTTRLVVRVAAGVPIGSRQQALSLAREQLESDSII
                     RNECAKLWTQLTPIEQDALLEVLTNAEAPSSIGSTQADLIAKGILIENNPPQVFSELF
                     GGYARRQRKLRQPAQAGVWVDVDAGEVWIDGERAPTLTDLEYRLLLLLYGRSNKICDK
                     YQIVEAVWGQDYIDEVDDARIEKLISRLRTKIERDAANPRYLITVRGRGYKLASV"
     gene            complement(4904..5572)
                     /locus_tag="VFF70_09720"
     CDS             complement(4904..5572)
                     /locus_tag="VFF70_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45013.1"
                     /translation="MALNSNEPENNSTSTPDENANRTFRMAVIALGGIALLGVVLIAV
                     FLLTNSGQRQQLVPQQQSVDSPNETATQIAQITPTDSPEPTETPKPPTATPTLPPTGA
                     PTQAAQPVVTSTGAATGAATGTKTAVGPATSTPKLPSASVVASSTAKPANTTVATKVA
                     TKTSTSAPVVAAGQLSGITPTAAATTPDKIAQTGAGDYLGFLLAGGLIVLLFVARRLR
                     TAQA"
     gene            complement(5686..6768)
                     /locus_tag="VFF70_09725"
     CDS             complement(5686..6768)
                     /locus_tag="VFF70_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45014.1"
                     /translation="MTDSVAYYPPETRVMPLTLIHRERLLPTPGKVLVSMQDRLQASQ
                     IVAQADATGEVRIVNIARALRVPTAEADRYLKVKEGDDVKAGAVLASRGVLSGARAVS
                     PADGFVSRVDKNTGRVLIQVVAKPFEMNAYLPGTVVSVIASRGVVIEAAGALIQGALG
                     FGGESFGVLHVTASEPTDVLRAQSIDAASHGAIVVGGAWIDESALQQGIQLQVRGIIA
                     GSLEGRLLEMARSLAFPIILTEGLGKLAMAGPIFKLLQGQAGREASISAITRTRWGIT
                     RPEIIIPLPADSKPALPPNVGTLLANNSRVRVVRGPQQGEVGEVASVVDKPRRIESGA
                     RVYGAEINLDASGKMFVPLANLEILR"
     gene            complement(6768..8528)
                     /locus_tag="VFF70_09730"
     CDS             complement(6768..8528)
                     /locus_tag="VFF70_09730"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HZY45015.1"
                     /translation="MEQQTQVHSILGVDIGSVNTQATLIARVEGTFRFVARAQSPTTL
                     EDPWSDVSIGVRHAIEQLTQIAARPMLNDKGDVISPEQPDGAGVDLCVITCSAAQPLR
                     VVLAGLVSDLSLDSLRRAAAGTYTWVEDVFGLLGTERLSDEARARRIMSIQPDVVCIA
                     GGTDGGAEEPVLDLVEAVTLGASLLDQAVRPKIIYAGNANLRSRVSQIVGQQTEILMA
                     EGNVRPNLETENLDPLMNELEVLHAHNKIGNMPGIAACAAWSALPMLSTARSLGHVIQ
                     YLSALDDQSRGALGVDVGASTTSIATGFKGNLHLSVRSDIGSAFGGTRLLREIGAAAI
                     LRWLPFELTEGELEAFLINKELHPLSIPQDTRELLIEHAIAREAIRAALRTAKPSWYT
                     DRQAALEDLMPLIDPIVGAGAVLARAPRPGQAALMLLDALEPIGVTTLNLDVYGLIPA
                     LGLAALAQPLSATQAIENNGLLTLGTAIVPVGRARLGEIILGVKISYEGGGDLEVEVA
                     AGTLEVLPLSLGQKAALRLAPRRGINIGYGPGRGVKLPDVNGGMVGLIIDARGRPLSL
                     PSNAKKRCQRIQQWLWDVGA"
     gene            complement(8556..9218)
                     /locus_tag="VFF70_09735"
     CDS             complement(8556..9218)
                     /locus_tag="VFF70_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45016.1"
                     /translation="MSIDQIGLILGAALSVIILSYLIGDNFLYRFFTHVLVGVGAAYI
                     VVTVVTDVLIPQMVDPLLQRAQSGQVVVAAAGLLGCILLLVKAWRPRLAGIGNISIGY
                     LVGVGAAVALGGALFGTLSAQIGAAGQLPPRSDPDFWPKVFFIAIAAFGTITTLLSFG
                     FYRVARDGLLSRVSTIGRFFLSAALGATFALVYVASVSLLIDRVQAIADVVNMLSKLP
                     KP"
     gene            complement(9215..>9409)
                     /locus_tag="VFF70_09740"
     CDS             complement(9215..>9409)
                     /locus_tag="VFF70_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45017.1"
                     /translation="VVGMGGAFVYGQYSGLSPLAIDVKATELQIRLESQTLAHWIMIA
                     IILLGMLSALFSRDGRRSAA"
BASE COUNT         2127 a         2803 c         2490 g         1989 t
ORIGIN      
        1 cgtccgctga agtcgcgtaa gccgcaaagg cgcggaatgt ctcgcgattg agatcggtta
       61 acttgtccgc ttccaacaac gccagcggat gatcctgcca caatgatcct tcgctcggat
      121 aaactgcgac gagatcggtg ccgtgttgat tggcccagat caacatttgc tcggacgcaa
      181 cgaatgcatc caaatagctg cggccattct gcaccacgcg ctgcgcaatg gcgtactccc
      241 cctcgccgta atagcgaacc gttttctcca gcgccgtcac atactgcaaa acgttgggcg
      301 attgcacatc atccgccgtg aggccgcgcg ttttattcgc gcccgcgtaa aattcagcaa
      361 gcgttgcgag caagcccgat gcggacgccg tcgagggatg actccacttg aaatttggat
      421 cggacgtggc aatcttcagg atgtcgctcc acccgatcgg tgtaccgggc cagccgagcg
      481 cttgcgcttt atcacgccac atagcgatga ccacgggcga aaccgcatac cgcaccgatt
      541 gtcccaccaa acccgtcgcc gattgcgtgg tcgcttcata atctcgatcg agctgatcga
      601 gccagatcga tgaatcgggc gacatggcct ggaagtttcc ggcgatggcc gcgtccagca
      661 tcgattccgg atcgagctgc acgatgttaa ccaccagcgt ttcgccggtc gatgctttca
      721 cgatcttgct gttgaaagta ttaaccagct gcttgaagag atcgcccttc tccggcgaga
      781 ccgcgatcgt cagtgtgggt tgcggacctt gcacaacgtt attgatgcct gagccaatgc
      841 ccttgaccac attcgagatc gaattgaaca cgcccgaagt caacggccag cacaacgcgc
      901 aggcggcgat gacgatgatc agcagcgtaa tccagccggt gggattgggt cgaggcgttc
      961 ggtaggagga tcgatagtga gacatggtga ttgtgattag taacgagtta tcagtaacgc
     1021 gtaccggcac gtcgttgcga ccctgtacaa agcagacagg gcaggttgtg gccgttaggg
     1081 cgatgtcgca atctcaaaat cgatcagaag attgccatgc cgcggcgcgg ctcgcaatca
     1141 cgaggatcaa cgcaccgcaa cattcagatc gaaccaacgc agcagcgctt gcagcacatc
     1201 tcgatcgggt gagcgcatgg ccgaggtgcg ttgcaccaca tcgaccacac ccgccgctga
     1261 ccatttggta aacggactgt cgggttgatc gacggtcaca tcggcgttga ccgggcgtag
     1321 tccgtaactc acggccttct gttgaaccgg cgcacttaac aggtactttt caaattcaag
     1381 tgccgcattt ttttcttgcg cactcgtctc aggaccaatc cacacgctga acggaaaatc
     1441 gaaccacgtc acgaacttgg gatagcgcac cactagcgga tctttccatc gatttgaaat
     1501 gccggccata ttcgccagta gatcgctctc cagcatttgt ccgccatcgc cgaccgaata
     1561 accgagcaat gcaaaatctt ccgcggtata agcgctcgcg ccactgatgt ccgtgacgga
     1621 actcatcaac tctttcagcc atttttgaaa atcgggcttg gtcacgtcag ccacgctgat
     1681 atcgggccgg ttgtaatatt cgcccgctgc cgcaaccatc gccgcgaggc cgccgacatt
     1741 cttgcgcgga ttcggcacga cgagtttgaa aaagccccag gccggttcgc cgccgagttc
     1801 cggccaaccg ccggatgcga tcgctgcatc atgcagcgtc aaccaattga tttcattgaa
     1861 ttttgtttcc aacacacgcg ctcggctgcc ataaatgccc cacgcaaaca acgacaacgc
     1921 gatcggcttg gcgcgatatt cgccatcggt gagaaacacg tcacggccca atcgatcttt
     1981 gtaggcagcg ttcgccaatt cgaccaagta acgtgaatcc ggaatccacg ccgtcggcac
     2041 cggatcaaac gcagcgctgt catagcggct caaggccgtc aggccatcca tcggaatgat
     2101 ggtgacgtga atcttgttgt tgtcgagttg atgattctcg gtgttgaatt gattggccgc
     2161 ggcagtgacc cacggttcga tcggcagggc gacgacgatg cgcacttcga gcgggccgct
     2221 gggcgccgcc ggaccgaccg ggttggagtt aaccgatcgt gcgatcaggc tgatcgcaat
     2281 gatcgcgatc gcgacaccga tcacgccgaa gaaaatccat cgagttcgat tcatgccatt
     2341 tactcctgcc caccgcgcta ttttaattgt atctgcttat ggctggcggg caagtcatcg
     2401 ggcggagccg aaaccgaccc cgcccgatta ttgattgaag ctcgtagggg cgaggcaacc
     2461 tcgcccttac ttaattgatg cccagtcgtt tcttgcgctc ggccaattga ttatcgagct
     2521 cttgtttgcc caacacctcg tccatttgat catcgagacg gctcgccagc atctcgctct
     2581 tggctgaagc tttgtccaac cgcgcgcgga tcgagtccgc catgcgcgtc acatcggcat
     2641 cgccgaggcc ggccaaatca tccaatgact tgattgtctt gacagtcagt tccttggact
     2701 tggcaagatc gagcagcgcc agcagctctt cgcgctcttg cttggtggtc gtcaacttcg
     2761 cttcaagctt gagcttggca ttcatcaagt tttgatattc aacttgctgc cgatcgtact
     2821 gttctttgta tgactcaacc agatgttgaa tggaattgag cttagattgt gccgcagttg
     2881 ccagatcttc gcggccctgc tgcaacaaag cgtcaatatc cttatcgagc ttggccgctt
     2941 gcgccgaaaa gtcatcgtat ttgcgtttca gcgttttgac ttcgccgccg accgtagccg
     3001 cggcgtcttc caatgcttcg agattgtttt ctgcctgacg gatatattca tccatcaccg
     3061 cgaccgaatt agccttgagt gccgagtcga ccatcgcgtg caggttggcc gaaatcaagg
     3121 ttttgacttt ttcgagcaga gatgccatga gattttcctc ctgaaggaat gttgaagaat
     3181 gaaaagtgac gtgtgacgtg tgacgtgtga tgcgtgatgc gtgatgcgtg atgcgtgatg
     3241 cgtgatgcgt gatgcgtgaa gaagaatatc acccagtgaa gcgggccgct tgacggctca
     3301 ccgacagttg gctgacagaa ttcctgaagc atcctgagcg gaactgcggc gtgagcgatg
     3361 cagtgcaatc gaaggcgcgg cgcatatgca ctgcccaacc ttcgcaaggt taaaacatcg
     3421 atcagactga agctaatttg tagccgcggc cgcgcactgt gatgaggtag cgcgggttcg
     3481 ccgcatcgcg ttcgatcttg gtgcgcaggc ggctgatcaa tttttcgatg cgggcatcgt
     3541 cgacttcgtc aatgtaatct tgaccccaca ccgcttcgac aatctgatac ttgtcgcaaa
     3601 ttttgttact gcgtccatac aacagcaata gcaagcgata ttcgagatcg gtcagcgtcg
     3661 gcgcgcgctc accatcgatc cacacttcgc cggcatccac atccacccac acgccggcct
     3721 gtgcaggctg gcgcagcttg cgctgtcgac gcgcataccc gccaaacaat tcgctaaaga
     3781 cttgcggtgg attattttcg atcaagatac ctttggcgat cagatccgcc tgggtagatc
     3841 cgattgaact cggcgcttcc gcgttcgtca acacttcgag caacgcgtct tgctcgatcg
     3901 gtgtgagctg cgtccaaagt ttagcgcact cgttgcgaat gatcgaatcg ctctccaatt
     3961 gttcacgcgc caagctcaac gcttgctggc gcgatccgat cggcacaccg gcagccactc
     4021 gcacaactaa gcgggtggtc gattgcaata agccgggatg accgcccgct tgcgccacaa
     4081 tgaaatccac ttcgtctcga tcgagctcgg cgccgtcttc tttgccccac gcttttacag
     4141 cgcggcgggc gatctcatcg gcgggcatac ccaatatcat ttgctgcccg acaaagagtt
     4201 cgcaaaattc gctggcttca gcatcatgcc gccgatcggc taacggggcg ccggtggcgg
     4261 tgacatacac cagtgactcg tatttatcgt gcagggcgcg gaggttgagg aagacgcggc
     4321 catcgagcgc gccgaacgga tcgtcaaact cgtcgaacag cagcgccacg cgccggttca
     4381 atttctcgca cagcagcgca atcccttcat tgaaactcag tggcgcgatg atcccgcccg
     4441 gccgatcggc atcgatcacc tggcgataca gtttttcaat cgcagcgatc aattccgccg
     4501 aaacgttggg caggtgcttt aaagcatcaa gggctgatcg gagggtgact tcatagaaag
     4561 cttgatcgct caatgccagc atcaaattgc aatcgacata cacggtgaaa gatcgattgg
     4621 atttgctatc gggttcagcg gcggcgcgca gcagtgttga tttgccgacg ttgctcaaac
     4681 ctatgatcga gcagcaagcc ccatcggtca gagtggattt taattgatcg agatcgttgc
     4741 ggcggaggaa aagtgagtca gacatgatga gggcgcttaa actggcagag gttgaagttc
     4801 agaagtgtcg ctcgacacgg aaacctccgc aaggttcttc aaaaagtaaa agtgggtcaa
     4861 ccgacctcat tggacagttg acccactcgt ttaatcatgc agcttaagct tgcgctgttc
     4921 ttaagcgacg agccacaaac aataacacga tcaaaccacc cgccagcaga aagccgagat
     4981 aatcacccgc acccgtctgg gcaattttat cgggagtggt agcagcagcg gtcggtgtga
     5041 tgccactcag ctgcccggcg gcaaccacag gcgctgacgt agaggtcttg gtggcaacct
     5101 tggtggcaac cgttgtgtta gccggcttgg cggtgctact cgctacaacg gatgcactcg
     5161 gcaatttcgg tgttgaagtc gcagggccaa cagctgtctt tgtgccagtc gcagcgccag
     5221 tcgcagcgcc agtcgaagtc acgacaggct gtgcagcttg cgtcggtgca cctgtgggcg
     5281 gcaaagttgg tgtggccgtc ggcggcttcg gtgtttcagt cggctcaggc gaatcagtcg
     5341 gcgtgatctg ggcaatttgc gtagccgttt cattcggcga atcgacggat tgctgctgag
     5401 ggactaactg ctgacgctgg ccggagttcg tcaacaagaa gactgcaatt aagaccacgc
     5461 ccagcagcgc gataccgccc agcgcgatga ctgccatgcg aaacgtgcgg ttggcatttt
     5521 catcgggagt gctagtgctg ttattctcgg gttcattact attgagagcc attatcgttg
     5581 ctcctgttga gataggggag attggaataa tatgcgcagc ctagatcaat cattccaacc
     5641 tccaaactcc gacttcaagt taaggtattg taacagaaac aagctttaac gcaaaatttc
     5701 aagattggcc aatggcacaa acatcttacc tgatgcatcc agattaattt cagcgccgta
     5761 cactcgcgcg ccgctttcaa tgcgccgcgg cttatcgacg acgctggcca cttcgccaac
     5821 ttcaccttgt tgtggcccgc gcacgacacg cacacgacta ttattggcca gcagggttcc
     5881 aacattcggc gggagcgcgg gcttggaatc agccggtaaa ggtatgataa tctccggccg
     5941 ggtaatgccc cagcgcgtgc gcgtgatcgc gctgatcgat gcttcgcgtc cagcctggcc
     6001 ttgtaaaagt ttaaagatcg gtcctgccat cgccagctta cccagccctt cggtcagaat
     6061 gatcggaaat gccaggctgc gcgccatttc caataagcgc ccttccaggc tgccggcaat
     6121 gatgccacgc acttgtagct gtatgccttg ctgcaatgca gattcatcga tccacgcgcc
     6181 gccgacgacg atcgcaccgt gcgacgcagc atcgatcgat tgtgcgcgta acacatcggt
     6241 cggctcgctg gccgtgacat gcagcacacc aaacgattcg ccgccaaagc cgagcgcgcc
     6301 ttgaatgagc gcgcctgccg cttcgatcac cacaccgcgt gaagcgatga cactgaccac
     6361 cgtgccgggc aaatatgcat tcatctcgaa cggcttggcc actacttgaa tcaatactcg
     6421 gccggtattc ttatcgacgc ggctgacaaa cccatcggcg ggcgaaacgg cgcgcgcacc
     6481 gctaagcacc ccgcgcgaag caagcaccgc acccgctttt acatcatcac cttcttttac
     6541 tttcaaatag cgatctgctt ccgccgtcgg tacgcgcagc gcgcgcgcaa tattgacgat
     6601 gcgcacctcg cccgtcgcat cggcttgcgc cacgatctgt gaggcttgca agcgatcctg
     6661 catgctgact aaaactttgc cgggcgtggg caacaggcgc tcgcggtgaa ttaacgtcag
     6721 tggcatcaca cgcgtttcag gcggatagta agcaaccgaa tcagtcatta agcacccaca
     6781 tcccataacc attgctgaat acgctggcag cgcttcttgg cattcgacgg caggctcagc
     6841 ggccggccgc gtgcatcgat aatcaatcca accataccgc cattcacatc cggcagtttt
     6901 acgcctcgac cgggaccata accaatattg atccctcgac gcggcgccaa acgcagggca
     6961 gctttctgcc ctaatgacag cggcagcact tccaatgttc cggcggctac ttccacttcc
     7021 aaatcgccgc cgccttcgta gctgatcttt acacccagga tgatttcacc gagccgcgct
     7081 cgaccaactg gcacaatggc ggtgcccagc gtgagcagtc cgttattctc gatcgcttgg
     7141 gtggcgctca acggctgagc aagtgcagcc aggcctagcg ccgggatcaa accatacaca
     7201 tccaaattga gcgtcgtcac tccgatcggt tccagcgcat ccaacaacat caatgccgct
     7261 tgaccgggcc gcggtgcgcg cgcgagcact gcgcccgcgc cgacgatcgg atcgatcaac
     7321 ggcatgagat cttcaagcgc cgcttgccga tcggtgtacc agctgggctt agccgtgcgt
     7381 agtgcggcgc ggatcgcttc gcgcgcgatc gcatgttcga tcaacaactc gcgcgtatct
     7441 tgcggaatac tcaagggatg cagttcttta ttgatcagaa acgcttcaag ctcgccttcg
     7501 gtcaactcaa agggcagcca gcgcaagatc gccgccgcgc caatttcacg cagcaaacgc
     7561 gtaccgccga aggcgctgcc gatgtcactg cgcaccgata aatgcaaatt gcctttaaag
     7621 ccggtggcga tcgacgtcgt ggacgcgccc acatccacac ctagcgcccc gcgcgattga
     7681 tcatctaacg cgctgagata ttgaatgaca tgccccagac tgcgcgccgt cgacaacatc
     7741 ggcagcgcac tccacgctgc acaggcggca atgccgggca tgttgccaat cttgttgtga
     7801 gcgtgcagca cttccaactc attcatcaac ggatcgagat tttccgtttc caaattgggc
     7861 cgcacgttgc cctcggccat caagatttca gtctgctgtc cgacgatctg acttacgcgc
     7921 gatcgcaaat tggcattgcc cgcatagatg atcttgggcc gtaccgcttg atccagcagc
     7981 gacgcgccca acgtaaccgc ttccacgaga tcgagcaccg gctcttcagc gccgccatct
     8041 gttccgccgg cgatgcatac cacatccggc tgaatggaca tgatgcgccg cgctcgagct
     8101 tcatcactga gccgttcagt gcccaacaga ccgaacacat cttcgaccca agtgtaagtg
     8161 ccggcagccg cacgccgcag actgtctaaa ctcaaatcgc tgaccagccc ggctaagaca
     8221 actcgcaagg gttgcgctgc actgcaggtg atgacgcaca gatccacacc cgcgccatct
     8281 ggctgctccg gtgaaatgac atcgcctttg tcattgagca tcggccgcgc cgcgatctga
     8341 gtgagctgct caatggcgtg acggacgccg atcgaaacat ctgaccacgg atcttccagc
     8401 gtcgtcggtg attgcgcgcg cgccacaaag cggaacgtgc cttccacgcg tgcgatgagt
     8461 gtggcctgcg tattcacgct gccgatatca actcccagaa tcgaatgcac ttgagtctgc
     8521 tgttccatat ttccgatcac acaacccaat atcgattagg gcttaggcag ttttgacaac
     8581 atattgacta catccgcaat cgcctgaacc cgatcgatca acaagcttac actggccaca
     8641 tacactagcg caaaagtcgc cccgagcgct gccgacaaga agaagcggcc aatggtgctg
     8701 acgcggctta ataagccgtc gcgtgccacg cggtaaaaac caaacgataa caatgttgtg
     8761 atcgtgccga aggcggctat cgcaatgaag aaaacctttg gccaaaaatc tggatctgaa
     8821 cgtggcggca gttgaccggc agcaccaatc tgcgcgctca atgtgccaaa caacgcgccg
     8881 cccaacgcca ccgccgcgcc gacgcccacc agataaccga tcgaaatatt tccaatgccc
     8941 gctaagcgcg gccgccaagc tttgactaac aacaagatac aacccaacaa tccagcggca
     9001 gccaccacca cctgcccgga ttgagcgcgc tgcaacagcg ggtctaccat ttgcgggatc
     9061 agcacatcag tcactactgt gacaacaatg taggccgcac ccacgcccac taacacatgc
     9121 gtaaagaagc gatacaagaa attatcgccg atcaagtaac tcaaaatgat cacgctcaga
     9181 gctgcgccaa ggatgaggcc aatctggtca atgctcatgc agccgatcgc ctcccgtcgc
     9241 gcgagaacaa cgcgctgagc ataccgagca aaataatcgc gatcatgatc cagtgagcca
     9301 aagtctgtga ctccaagcga atctgcagct cggtcgcttt cacgtctata gccagcggcg
     9361 acaatcccga atactgacca tacacgaacg cgccacccat gccgacgac
//
LOCUS       DASRTJ010000477         5889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_29953,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000477 DASRTJ010000000
VERSION     DASRTJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29953"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFF70_09745"
     CDS             <1..704
                     /locus_tag="VFF70_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY45018.1"
                     /translation="VASLVQELRTPMTSINGYTDLLLGESVGILGAMQRQFLQRVKAN
                     IERMDAMLQDLIGITAIETGQLKIEPESIDVSEVIEESVMNAAAVFRDRELSIRIELA
                     EGLPKLKADRDSLHQIISHLLSNAALCSPNGSEVIVRAQVQPEMSDYLLFSVADHGGG
                     IATEDRQRAFHRMYRADNPLIQGLGETGIGLSIAKTLVEAQGGRIWVDSVMGDGSTFT
                     FVLPITPRTEQVESV"
     gene            701..1648
                     /locus_tag="VFF70_09750"
     CDS             701..1648
                     /locus_tag="VFF70_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45019.1"
                     /translation="MNTSNRSKQLLEGLAIGAIVIFTLAGAVLLSTRNTIALPNTSPT
                     PIVPATPSATAAPINTPGPTINAATHAPTVTQSATATSTGTSTPTPAPTRCPPPIGWQ
                     PYTVGSFDTLFLIAQKFNVDPNMLSRANCLSNYAVTVGQTIYVPSTLPTATTAPCYPP
                     YYWAIYSVQPGDTLTAIAARYNISVYQLWRANCLASSFIYVGQLLRVPPIMIYPTATP
                     TFIPSATPTATPTNTPTPSITPSATSTVTPTPSATSVPTIGSSPTPTDTSTPTPTDTT
                     VPPSPTPTDTPTTIPPSPTDTATLVPPTATDTPTPPSTP"
     gene            complement(2051..3253)
                     /locus_tag="VFF70_09755"
     CDS             complement(2051..3253)
                     /locus_tag="VFF70_09755"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZY45020.1"
                     /translation="MTKSPNLQIQREPTRKQLSRAEREAKQRRRIWLLVFGVLGLATL
                     ILVYGYLYQAVFILNEPVANVNGEIISTRQFQDRVRLAGKQLQQQIQVAYSLNDQNSI
                     DYYVNQLSDPVTLGGQLINSMVDDLLLKQAAPTFGVSVSVDEVQLNIEQQLRYDRNPP
                     TPAPTSTPRPTPTASGPITQTPTPSITPFPTATPVTLDAFNKLYQEQLGQLAALGLGE
                     QEYRDLVQTSLLVDKVRTAIESTVVTTTDQIKFKYIRAANADAVTVTRDINAQDFATI
                     YQAVLSGTYPITTVQATETSDWVPHDEISATTEFGPVLADTLFATPVSQTMALVNAAG
                     TATYVALILDHQVQPLNASFLQSRQQAAIDDWLAQRRKPEFYFTWNDRVPVVPVPTPL
                     SQTQSTSP"
     gene            complement(3397..3750)
                     /gene="rpsR"
                     /locus_tag="VFF70_09760"
     CDS             complement(3397..3750)
                     /gene="rpsR"
                     /locus_tag="VFF70_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZY45021.1"
                     /translation="MADQDMRVESSSGGAASQRRAYNSGGGAGRTDRRGGPGGSGGGR
                     YMARPKVCQFCVDKVKAIDYKQGDMLRRFVSERGKIRPRRQTGTCARHQRFLTTALKR
                     ARHLAMLPYVGNQAR"
     gene            complement(3871..4272)
                     /locus_tag="VFF70_09765"
     CDS             complement(3871..4272)
                     /locus_tag="VFF70_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZY45022.1"
                     /translation="MTRGLNKVMIIGRLGRDPEMRYTASGKPVTVFSVAVNRTWTTGD
                     GDRHEETEWFNVVAWGNLAEICKQHLHKGQSVYIDGRLQTRSWDDPDGKKHYRTEIVA
                     NEMIMLGDRRGEGGEEPIVDSPTIGEEEFPF"
     gene            complement(4300..4596)
                     /gene="rpsF"
                     /locus_tag="VFF70_09770"
     CDS             complement(4300..4596)
                     /gene="rpsF"
                     /locus_tag="VFF70_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZY45023.1"
                     /translation="MANTRPYEVTFIVRPDVDETGLTGVIDKVKGWITASGGSVSKTD
                     LWGRRRLAYPIKKMTEGQYVLLLADLPAQSLAGLERDLRLSEEILRFLLVRAGE"
     gene            complement(4875..>5889)
                     /locus_tag="VFF70_09775"
     CDS             complement(4875..>5889)
                     /locus_tag="VFF70_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45024.1"
                     /translation="GRRRLQAVLTSTSDAVIVTDKENRVLLCNPAAEVAFGLAAGSAV
                     GQAVSMVWPEATLDRLLKLDDGASSRTEEIALPDGRTLFGSASGIISGDGQLNGRVVV
                     LRDITQLKELDAMKSEFVATVSHDLRAPLTYMRGYTTMLPMVGTLSPKQQDYLEKIML
                     GIEQMTELIDDLLNLGRIEAGIGLVRKTCSLAEVARSVIDAMQGQASAHDVKLVTGKL
                     SLKTIEGDLGLVHHAVMNLVDNAIKYTSKGGTVTVSVEEGDGNSVVVVKDTGLGIAPG
                     DQVRLFERFYRVRRRETIDIKGTGLGLAIVKSIADWHHGRVWVESQIGVGSTFYLLLP
                     MKA"
BASE COUNT         1308 a         1819 c         1454 g         1308 t
ORIGIN      
        1 tcgtggcttc gctggtgcag gaactgcgca cgcccatgac ttcgatcaac ggctacaccg
       61 acttgttgtt gggcgaatcg gtgggtattc tcggcgccat gcagcgccaa tttttgcagc
      121 gcgtcaaagc caacatcgaa cgcatggatg cgatgctgca agacttgatc ggcatcacgg
      181 cgatcgagac cggccagctc aagatcgaac cggaatcgat cgatgtttcg gaagtcatcg
      241 aagaatcggt gatgaacgcg gcggctgtgt tccgcgatcg agagctgtct atccgcatcg
      301 agctggccga aggcctgccg aaattgaagg ccgatcgaga cagcctacat caaatcattt
      361 cgcatctctt atcgaacgcc gcactgtgct cgccgaatgg cagtgaagtg atcgtgcgcg
      421 cgcaagtgca gccggagatg tcggactatc tgttgttcag cgtggcggat cacggcggcg
      481 gcatcgccac ggaagatcga cagcgcgcat tccatcgcat gtatcgcgcc gacaatccgt
      541 tgattcaagg cctcggcgaa accggcatcg gtctgtccat cgccaaaaca ttggtggaag
      601 cgcagggcgg ccgcatctgg gtcgacagcg tcatgggcga tggctcgacg tttacatttg
      661 tcctgccgat cactccacgc actgagcaag tggaatcagt gtgaacacat ctaatcgatc
      721 gaagcaattg ctcgaaggat tggcgatcgg cgcgatcgtg attttcacgc tggccggagc
      781 cgtgttactc tcgacgcgta atacgatcgc actaccgaac acctcgccaa ccccgatcgt
      841 tccggcgacg cccagcgcga cggctgcgcc gatcaacaca cccgggccaa cgatcaatgc
      901 cgcgactcat gcgccgacag tcacgcaatc agccaccgcg acttcaaccg ggacatcgac
      961 gccgacgccg gcccccaccc gctgtccgcc gccgatcggt tggcaaccgt ataccgtcgg
     1021 ctcgttcgat acattgttcc tcatcgcgca aaaattcaac gtcgatccga atatgctgtc
     1081 aagggcgaat tgcttatcga attacgccgt taccgtcggc caaacgatct atgtaccgtc
     1141 cacccttcca acagcgacga ctgcgccgtg ctatccgccc tattattggg cgatctacag
     1201 cgtgcagcct ggtgacacac tgaccgcgat tgctgcgcgc tacaatattt ctgtctatca
     1261 attgtggcgg gccaattgcc tggccagcag tttcatttac gttggacaac tgctgcgcgt
     1321 cccgccaatt atgatctatc caaccgcaac gcccacattt attcctagcg caacaccaac
     1381 cgcaacgccg acgaatacgc ccacgccatc gatcacgccc agcgcgacga gcaccgttac
     1441 gcccacacca tccgcgacca gcgtgccaac gatcggatca tcgcccacac cgacggatac
     1501 ctcgacgcca acaccgaccg atacaactgt gccgccctcg cccacaccta ctgatacacc
     1561 cacgacgatc ccgccctcgc caaccgatac ggcaacactg gttccaccca cagcaacgga
     1621 tacgcccacg ccaccgagca cgccgtgaaa gaaagttgat cagggattaa cccctaccct
     1681 ggccctcccc ccgattctca ggggagggaa aagagaaatg cccgatctac aatgcgatcg
     1741 ggctgcttgt ttgagtcatc tactctgcct cacgcttgca tcgatcgatt aagcacgcga
     1801 tgccttgcca tctcccgatt ttgtgaatcg ggagatggac aacgataatg ctagcgatga
     1861 atctgcatcg gggtgaggca gattgcccct cccgcgtcgc gtatgaatct agctcagtgc
     1921 gtaaacacca aattgcattc agtgtaactc aattcacgtc aatcgcatta caacaaaaaa
     1981 ctccgactca cttatgagtc ggagtttccc aaatccataa ttcctgatca actaatcccc
     2041 aatccctact ttacggactt gtcgattgcg tctgcgacag cggcgtgggg actggcacca
     2101 ccggcactcg atcgttccag gtgaaataga actccggctt ccgtcgctga gccagccaat
     2161 catcgatcgc tgcttgctgg cggctctgca agaacgatgc atttaatggc tgcacttgat
     2221 gatccaaaat cagcgccacg taagtggccg tgcccgccgc attcaccaaa gccatggttt
     2281 gactgaccgg agtcgcgaac agtgtatcag ccagcacggg accgaattcg gtcgtggctg
     2341 atatttcatc atgcgggacc caatccgacg tctctgtggc ttgcacggta gtgatcggat
     2401 aggtgcctga caaaaccgct tgatagatcg tcgcgaaatc ttgcgcgttg atatcccgcg
     2461 tgactgtcac tgcatccgca ttcgcggccc gaatatactt aaacttgatc tgatcggtcg
     2521 tcgtcaccac cgtcgattcg atcgccgttc gaactttatc gactagcagt gaagtctgca
     2581 ccaagtcccg gtattcctgc tcgccaagac cgagcgcggc caactgtcct aactgttctt
     2641 ggtacaactt attgaaagca tccaaggtta ccggcgtcgc cgtcgggaac ggtgtgatcg
     2701 aaggcgtcgg tgtttgggtg attggaccac tcgccgtcgg cgtcggacgc ggcgtgctgg
     2761 tcggcgccgg tgtcggcgga ttgcgatcgt agcgcagctg ctgctcgata ttcagctgca
     2821 cttcatccac gctgaccgac acaccaaagg ttggcgcggc ctgtttcagc agtaaatcat
     2881 ccaccatgct attgatcagt tggccgccca gcgttaccgg atcgctgagc tgattaacat
     2941 agtagtctat actattctga tcgtttaagg agtaggcgac ttgtatctgc tgctgtaatt
     3001 gttttccggc taaccgtacg cgatcttgga actggcgtgt agaaatgatc tcgccgttca
     3061 cattcgccac cggttcattc aaaatgaaca cagcctgata taggtagcca tacaccagaa
     3121 tcagcgtcgc caatcctaaa acgccgaaga caagcagcca aattcggcgg cgctgtttcg
     3181 cctcccgttc cgcgcgcgac aactgcttgc gcgtcggctc tctttgaatc tgaaggttag
     3241 gtgatttcgt catatcgtct cagcacaggc agctaagttc acgcccagac gcgaagccgc
     3301 aatataaact gaaggccggt tagcgacttg gcgcgctgac cattgtagtc agcgcctcga
     3361 tcaagatttt tattggcgcg ctgactattg attcttttag cgagcttgat tgccaacata
     3421 cggcagcatc gccaaatgcc gcgcacgctt cagcgccgtg gttaaaaacc gctgatgccg
     3481 ggcgcacgtc cctgtttggc ggcgcggtcg aatcttgcca cgctcactca caaagcggcg
     3541 cagcatatcg ccctgcttgt aatcgatggc cttgacttta tcgacgcaga actggcaaac
     3601 cttcgggcgt gccatataac gaccgccgcc actgccaccc ggcccgccgc gtcgatccgt
     3661 tcgacctgct ccaccaccgc tgttatacgc ccggcgctgc gacgccgctc caccagaact
     3721 tgattcaact cgcatatctt gatcagccat gcataccctc caaagatgcg tggatcgatc
     3781 ggcttacccg tgcggcaccc atgcggtaca catgcagtaa agcacgtatt gcgtaccgaa
     3841 gcccgcagtg cgtaccgtcg atcacccgcg ctaaaacgga aattcttctt ccccgatcgt
     3901 gggtgaatcc acgatcggtt cctcgccgcc ctcgccgcgt cgatcaccca gcatgatcat
     3961 ttcgttggcg acgatctcgg ttcgataatg tttcttgcca tccggatcat cccagctgcg
     4021 ggtctgcaag cggccatcga tatacacact ctggcctttg tgcagatgct gcttgcagat
     4081 ctcggccaga ttaccccagg ccaccacgtt aaaccactcg gtctcttcgt gccgatcgcc
     4141 atcgccagtc gtccaagtcc gattgacggc tacactaaaa accgtcaccg gcttgccgct
     4201 cgccgtgtag cgcatttcgg gatcgcgtcc caatcggcca atgatcatga ccttgttcaa
     4261 tccacgtgtc atgccaccct cccgcttgct ggaatagaat tactcgccgg cgcggacaag
     4321 caagaagcgc aggatctctt cgctgagtcg gagatcgcgc tccaacccgg ctaaagactg
     4381 cgccggtaaa tcggccagca acaacacata ctggccttca gtcattttct tgatggggta
     4441 tgctaaacgc cggcggcccc atagatcagt tttggaaacc gatccgccgc tggcggtgat
     4501 ccatcccttg actttatcga tcacaccggt cagtccggtt tcatcgacgt caggtcggac
     4561 aatgaacgtg acttcgtagg ggcgagtgtt cgccataatt ctcctttcca tgggattgcc
     4621 ctcaggctca cactgccgag agcggaagga gcgtacactt atccaatatt gaattgtagt
     4681 ctacgcaacg aggcaggatt ttagcacaac tgccggattt cgtcaatgca ataatcgctg
     4741 tattctccag tgcctggtgc ggtattttct tccttcatta tcatccttca caacgcatca
     4801 aacaatatcc gctgatcatt gagcattgaa cttttatcag catgaattgt gcatgatgct
     4861 tgatgcaaca agcatcatgc cttcattggc agcaatagat agaacgtcga gccaacaccg
     4921 atctggctct ccacccacac tcgaccatga tgccaatcgg caatcgattt gacgatcgcc
     4981 agccccaggc ccgtgccctt aatatcgatc gtttcgcggc gcctaacacg atagaatcgc
     5041 tcaaataagc ggacctgatc acccggcgca atgccgaggc cggtatcttt cactacgacc
     5101 acactattac cgtcaccctc ttcgaccgag acggttaccg tgccgccttt ggacgtgtac
     5161 ttgattgcat tgtcgactag attcatcacc gcatgatgga ccaaaccgag atcgccttcg
     5221 attgttttca gcgacaattt gccggttacc agtttgacat cgtgtgcgct ggcctgcccc
     5281 tgcatcgcat cgattacact ccgggctact tcagctaatg agcaagtttt ccgaactaag
     5341 ccgatgcccg cctcgatgcg ccctaaattg agcaggtcgt cgatgagttc ggtcatctgc
     5401 tcgatgccca acatgatctt ctccagataa tcctgctgtt tcggcgaaag tgtccccacc
     5461 atcggcagca tcgtcgtata gccgcgcata taagtcaatg gcgcgcgcag atcatgactg
     5521 accgttgcga caaactcgct cttcatcgca tccagctcct ttagttgcgt gatgtcgcgc
     5581 aacacaacca cccgcccgtt cagctgccca tcaccgctga taatccccga tgcgctgccg
     5641 aacaatgtgc ggccatccgg tagagcaatt tcctcagtgc gtgaacttgc gccatcgtcc
     5701 aatttcaaca agcgatccag cgtagcctcc ggccaaacca tcgaaactgc ttgaccgaca
     5761 gctgaaccgg ctgctaatcc gaatgcaact tcggcggctg gattgcaaag cagcacgcga
     5821 ttctctttat ccgtgacgat gacggcgtcg ctggtgctgg tcaacaccgc ttgcaagcgg
     5881 cggcgtcca
//
LOCUS       DASRTJ010000478         1749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_105315,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000478 DASRTJ010000000
VERSION     DASRTJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105315"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFF70_09780"
     CDS             complement(<1..349)
                     /locus_tag="VFF70_09780"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZY45025.1"
                     /translation="MSNSDQLTAYPEVLALLHDLLTSARSTLGDYFIGMYLFDSLANG
                     GFDRESDVDVLIVTDDVLSPDLFVALQAMHARIAALDEWCATQLEVSYIPQTALRRFD
                     PANNLHPHIDRGRA"
     gene            complement(498..1022)
                     /locus_tag="VFF70_09785"
     CDS             complement(498..1022)
                     /locus_tag="VFF70_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45026.1"
                     /translation="MKRSLMIGLAILPAAVLIAIATLGTPGILGVHVPTTQDQVSSAI
                     DSYLQYRAPRARSINSIQQLIPASLPGNFTSRMSKATFGNGNYFKSDFHSTHIPVMWL
                     GQKPLPYPPTDVWCARLLPSGNSYNSYPDIVFIAQHEDDYHAEWIVHDPATETRQQLT
                     ADLAALGCSEVEQP"
     gene            complement(1236..1460)
                     /locus_tag="VFF70_09790"
     CDS             complement(1236..1460)
                     /locus_tag="VFF70_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HZY45027.1"
                     /translation="MPPKRIEIKPGLRVQIIEKQNQRSGQLTEGVVARILTSSPQHPH
                     GIKVMLDNGKVGRVQAIVPDAQSIKPATTL"
     gene            complement(1512..>1749)
                     /locus_tag="VFF70_09795"
     CDS             complement(1512..>1749)
                     /locus_tag="VFF70_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45028.1"
                     /translation="LANSTSILIAHRLSTVKNVDRIIVMRLGEIIEEGNHDQLLTQRG
                     HYAELYNTYFRHQSIDYRPGVFDGSIQVAIAQNQ"
BASE COUNT          428 a          423 c          488 g          410 t
ORIGIN      
        1 tcgcccggcc tcgatcgata tgcgggtgga gattattcgc cggatcgaaa cggcgcagtg
       61 cggtttgtgg aatgtaagag acttctaact gcgttgcgca ccactcgtcg agcgcggcga
      121 tgcgggcatg catcgcttgt agagcgacga agagatcagg tgagagaacg tcatcggtca
      181 caatgaggac atccacatca ctctcacgat cgaatccgcc gtttgcgagt gagtcaaaca
      241 gatacatgcc gataaaataa tcgcctaaag tggatcgcgc acttgtcagc aagtcatgca
      301 gcagagccag cacttcggga taggccgtaa gttgatctga gtttgacatc tgtcgcagcg
      361 aaggaaggat aacaccacaa tcaataggtc accttacgaa agtcttgatt acggtcattc
      421 ccgcgaaagc gggaatccag cgcaaaatga ctggaaaccc acctttgcag gtgtgacggt
      481 gcgttatctg gtcgcgatca tggttgctca acttcgctgc aaccaagcgc cgccagatca
      541 gcagtcaact gttggcgcgt ctcggttgcc gggtcatgga caatccattc agcgtgataa
      601 tcatcttcat gctgcgcgat gaacacgatg tccggataac tgttataact gttgcctgag
      661 ggcagcaaac gggcgcacca cacgtccgtc ggcggatagg gcaaaggctt ctggcccagc
      721 cacattacgg ggatatgggt ggagtgaaag tcactcttga aatagttgcc attaccgaat
      781 gtagctttgc tcatccgcga tgtgaaattt cctggcaagc tagccggaat caattgttga
      841 atggagttga tcgatcgtgc ccgaggcgcg cgatattgca gataactatc gatcgcgctg
      901 ctgacttgat cttgagtcgt tggaacgtga accccgagaa tcccaggagt cccaagcgtg
      961 gcgatggcga tcaacacggc cgccggtaag attgccaggc cgatcatcaa agaacgtttc
     1021 atgctgacct cctgtgaagc gccaactatc gaatttcgat atcagtataa ccccgattga
     1081 tgggcagtca atccgatcgg aattattcgt atcgcgatca gcggaatagg cagatgagaa
     1141 ggaataaaga attgatagca gcatcatctg tgagcctgcg cgatttttag agacgagcgt
     1201 aagcagaatc tgctgttaat gcaagtcttt ctgccttaaa gtgttgttgc gggtttgatt
     1261 gactgtgcat cgggcacaat cgcttgcacg cggccgacct tgccgttgtc gagcatcacc
     1321 ttgatgccat gcggatgctg cgggctgcta gtgaggatgc gcgcgacaac gccttcggtc
     1381 aattgaccgc tgcgttgatt ctgtttctcg atgatctgca cgcgcagacc gggttttatc
     1441 tcgatgcgtt taggcggcat agacgtaaaa aggaggaagg cggacgaatg aaggataaaa
     1501 aatgcaacgc tttattggtt ctgtgctatg gcaacttgaa tcgatccatc gaacacgccc
     1561 ggtcggtaat cgatcgattg atggcggaag taagtgttat acagttccgc gtaatgacct
     1621 ctctgcgtca agagttgatc gtggttgcct tcttcgatga tctcgcccag ccgcatgaca
     1681 atgatccgat cgacgttctt cacggtcgaa agccggtgcg cgatcaagat cgaggtgctg
     1741 ttcgccaga
//
LOCUS       DASRTJ010000479         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_105336,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000479 DASRTJ010000000
VERSION     DASRTJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105336"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VFF70_09800"
     CDS             <1..1482
                     /locus_tag="VFF70_09800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZY45029.1"
                     /translation="GIMGGLETEVDDHTTNVLIEAANFNMINIRQTTQQMKLPSEASA
                     RFGRGIHPAMALRGNIRSANLIQQLGGGTIDTGSVDVYPHPAETITIDLALSEVKRIL
                     GFEIEIDQIVHILESLEFKSEIHTPQSAISVSVPDHRIDCTGPHDLIEEIARIYGYDR
                     FPQVGMTDEMPPQRSNPSLEFEERVRDILVQAGLTEAITYSMTTPQREAKLLPGHRPD
                     DRPYVEIANPISAERTHMRHTLLASLLEVVAQNVRHHEYVAMFELNKVYLASEDGPLP
                     DEPRRLSIVMTGPREPLSWKGGDRNPIDFYDLKGVIEALLRGLHVEGEDFEATEHPTY
                     YPGRTARLKVNGTLIGVLGELHPLVREGFDFPDQTAVVAAELDLDELSKQISAIVRVA
                     EVPRFQAVIEDMAIIVDEKVPASKVLEVIKRGAGGEKNDSLLKHAELFDVFKGDRIGA
                     GKKSLAYRLTYQADRTLTDPEVVKVRELIIKRLKDEVDAVIRG"
     gene            1643..>1922
                     /locus_tag="VFF70_09805"
     CDS             1643..>1922
                     /locus_tag="VFF70_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZY45030.1"
                     /translation="MDWQQNLITNDNDILELARSSHRVAVLGIRSEKRADRAAFYVPE
                     YLASVGVDVIPVPVYEPETKEILGKEVYRRLIDVPGEIDLVDVFRRSED"
BASE COUNT          466 a          453 c          556 g          447 t
ORIGIN      
        1 ggcatcatgg gcggcctgga aaccgaagtg gacgatcaca cgacaaatgt tctgattgag
       61 gctgcgaatt tcaacatgat caatattcgc cagacgacac agcagatgaa gttaccgagc
      121 gaagcttcag cgcgattcgg acgcggtatc catccggcga tggcgctgcg cggcaacatc
      181 cgatcggcca atctgatcca gcagttgggc ggcggtacga tcgatacggg tagcgtcgat
      241 gtctatccac atccggcgga aacaatcacc attgaccttg ctctttcgga agtcaagcgg
      301 attcttggtt tcgagattga gattgatcag atcgttcaca tccttgaatc gcttgaattc
      361 aaatccgaaa ttcacactcc gcaatccgca atatccgtca gcgtgcctga tcatcgcatt
      421 gactgcaccg gtccgcacga tctgatcgag gagatcgcgc gcatctatgg ttacgatcga
      481 ttcccgcaag tcggcatgac cgatgagatg ccgccgcagc gatcgaaccc atcgcttgaa
      541 tttgaagagc gtgtacgaga tatactcgtt caagcgggtt tgaccgaagc gatcacttat
      601 tcgatgacga cgccgcagcg tgaagcaaag ttgctgcctg gtcatcggcc tgacgatcga
      661 ccgtatgtgg agattgcgaa tccaatcagc gccgagcgaa ctcacatgcg tcatacatta
      721 cttgcttcac tgctagaagt ggtggcgcag aatgttcgac atcatgagta tgtagcgatg
      781 ttcgaactca acaaagtcta cctcgcgtcc gaagatggac cgctgcccga tgagccgcgt
      841 cgtttatcga tcgtgatgac tggcccgcgt gaaccgttgt cgtggaaggg tggtgatcgc
      901 aacccgatcg atttctacga tctcaaaggg gtgatcgaag cgttgttgcg cggcttgcac
      961 gtcgagggcg aagattttga agcaactgaa catcccacgt actatccggg acgcaccgcc
     1021 cgtttgaaag tgaatggaac tttgatcggt gtgttgggcg aactgcatcc gttagtgcgg
     1081 gaaggttttg attttcccga tcagactgcg gtggtggccg ccgaactcga tctcgatgag
     1141 ttgtctaaac aaatatcggc gatcgtgcgc gtggcggaag tgccgcgttt ccaggcggtc
     1201 attgaagaca tggcgatcat cgttgatgag aaagtccctg ctagtaaggt gttggaagtt
     1261 attaagcgag gagcaggggg cgagaagaat gattctttat tgaagcacgc cgagttgttc
     1321 gatgtcttca agggcgatcg aataggcgcg ggcaaaaaaa gtttggccta ccgcctgacg
     1381 tatcaagccg atcggacgtt aaccgatccc gaagtggtca aagtccgcga attgatcatt
     1441 aagcggctca aagatgaagt tgatgcagtg atacgaggct aaatattttc gttgtcattt
     1501 cgaggagcgc agcgacgacg cctagcggcc cacctgaacg caaagcgttc agggtatggg
     1561 cgaaatctct gtgacaattg ggattatgct tttatcggtg tagagatttc tcgcttcgct
     1621 cgaaatgaca gaggagggtt atatggactg gcagcagaat ctgattacga acgacaacga
     1681 tatacttgaa ctcgcgcgat catcgcatcg agttgccgtg ctgggcatcc gcagcgaaaa
     1741 gcgcgccgat cgagcggcgt tctatgttcc agaatatttg gcatcggtgg gcgtcgatgt
     1801 gatccccgtg ccggtgtatg aaccggagac caaagaaatc ttggggaaag aagtctatcg
     1861 acgtttgatc gatgtgccgg gtgagatcga tctggtggat gtgttcaggc gatcggaaga
     1921 ta
//
LOCUS       DASRTJ010000480         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_202016,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000480 DASRTJ010000000
VERSION     DASRTJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202016"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFF70_09810"
     CDS             complement(<1..602)
                     /locus_tag="VFF70_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZY45031.1"
                     /translation="MAAKVNPSKSVAPNGQKPGVPSRREREKVQRRDDLLRAAKKVFF
                     KKGLHTATIDDVAAEAEVGKGTVYLYFDTKETILAHLLLAGLEELVVLLEAAYASDQK
                     LAARTRLRRIANAYLEFFQTNPEYYRLMMAFDRGHFQETVDPKVYQQVYHRSLRGLEF
                     LVLAVQQAKEEGVLDVDDTRHAASVLWAGLNGVLVLLSHPL"
     gene            827..>2383
                     /gene="mutL"
                     /locus_tag="VFF70_09815"
     CDS             827..>2383
                     /gene="mutL"
                     /locus_tag="VFF70_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZY45032.1"
                     /translation="MPIRVLPSNVVSQIAAGEVVERPASAVKELIENAIDAGATDIAV
                     ETRDGGRSFIRISDNGSGLPASEVELAFNRHATSKLASADDLTHIKTLGFRGEALASI
                     ASVAKVTCVSRSREETIGTRIAIEGGQISERGSIGAPAGTVVTVENLFYTVPARLKFL
                     KSDTTERRQIDLLISRYAMAYPSVRFNLIQAGRTAFQTTGNGSLKDVLIAVYGLDIAE
                     QMIELPPPPQPSPDKSGEGIKVSGFISAPALSRANRADMTIFVNGRWVQDRGLNAAVL
                     QAYHTLLMVGRYPVCIVRVEIDPEDVDVNVHPAKTEVRFRSNDAVFRVVQRNVRRTLI
                     DLAPIPQVQTQEPTSAWQPSFDRHDEIAVSRIEALRSLQPVDQDLANQSSVISHQPSS
                     LSDEPSVVSGLSPVINTHQPSLPPLRVIGQIGAMYIIAEGPEGMYLIDQHAAHERVLY
                     EKFMADKTSQHIAVQNLLDPLPMEFSPDESTLIEENIETLHTIGLHLDAFGGSTWLIR
                     SIPSLLAKDDI"
BASE COUNT          546 a          646 c          638 g          553 t
ORIGIN      
        1 agcggatggc tcaacaaaac caacacgcca ttcagtccgg cccacaacac gctggcagca
       61 tggcgcgtat cgtcaacatc gagcacacct tcttcttttg cctgctggac tgcaagtact
      121 aaaaattcaa ggccgcgcag gctgcgatga tagacctgtt gataaacttt cggatcgacc
      181 gtctcttgaa agtggcctcg atcgaaagcc atcatcaagc gatagtattc ggggttcgtc
      241 tggaaaaatt cgaggtaggc gttggcgatg cggcgcagtc gtgtccgcgc ggcgagtttt
      301 tgatctgagg cataagcggc ttcaagcagg acgacgagtt cttcaagtcc cgctaacaac
      361 aagtgggcca ggatcgtctc ttttgtatcg aaatacaaat agaccgtgcc tttgcccact
      421 tccgcttcag ccgccacatc atcgatcgta gcggtgtgca gccctttttt gaagaagact
      481 ttcttggcgg cgcgcagcag atcgtcgcgg cgctgaactt tttcgcgctc acgccgcgag
      541 ggcacgcctg gcttttgccc gttgggggca actgatttgg agggattgac tttagccgcc
      601 atcacgcctc atttactgac taacggtcag tcactgactg acagtcagta tattggagat
      661 tgagcggctt gtcaagacgg aatttgcggc gggcagagac gcatcccaac gcgcgtcctt
      721 cgacttcgca tcgatctctt cgagttcgct gcttcgctca ggatgctgac tgaattttct
      781 acttactcgt tacttttcac gcatcactca tggtacaatg ccgatcatgc ccattcgagt
      841 tctgccctcc aacgttgttt cgcaaatcgc cgccggcgaa gtggtcgaac gccccgcttc
      901 cgccgtcaaa gaattgatcg agaacgcgat cgatgcaggc gcaaccgata tcgccgttga
      961 aacgcgcgat ggtggtcgat cgttcatccg catttccgat aatggctcgg gcctcccggc
     1021 gagcgaagtc gagctggctt tcaatcggca tgcgaccagt aaactcgctt ccgccgatga
     1081 cctcactcac atcaagactc taggctttcg cggcgaagcg ctggcttcga tcgcgtccgt
     1141 agcaaaagtg acgtgcgtct ctcgatcacg cgaagaaacg atcgggacac ggattgcgat
     1201 cgagggcggg cagatcagcg agcgtggatc gatcggcgca ccggccggca ccgtcgtcac
     1261 cgtcgaaaat ctcttctata ctgtgcccgc ccgcttgaag ttcctcaagt ccgatacgac
     1321 cgaacgacga cagatcgatc tattgatcag ccgctatgca atggcatatc catcggtgcg
     1381 cttcaatttg attcaagccg gacgcactgc gtttcaaacc acgggcaatg gatcgttgaa
     1441 ggatgtttta attgcagtgt atgggttgga tatcgcggag cagatgatcg aattgccacc
     1501 tccaccccag ccctcccccg ataaatcagg ggagggaatt aaagtgagcg gtttcatcag
     1561 tgcgccggcg ttgtcacgcg caaatcgggc cgacatgacg atcttcgtga acggacgctg
     1621 ggtgcaagac cgcggcttga atgcggcagt gttgcaagcg tatcacacgc tgctgatggt
     1681 cgggcgttat ccagtgtgca tcgtgcgcgt cgagatcgat ccagaagacg tcgatgtgaa
     1741 tgtgcatccg gccaagaccg aagtgcgttt tcgcagcaat gatgcagtgt ttagagtcgt
     1801 gcagcgcaat gtccgacgca cgttgatcga tctagcaccg attccgcaag ttcaaacgca
     1861 agaaccaaca tcggcctggc agccgagttt tgatagacat gacgagatag cggtctcacg
     1921 aattgaggct ttaagatctc tacaaccagt tgatcaggat ttagccaatc agtcatcagt
     1981 catcagccat cagccatcgt ctctcagcga cgagccatct gtagtcagtg gtctgtcgcc
     2041 ggtgatcaac acacaccaac cctccttacc tccgttaaga gtcattggcc aaatcggtgc
     2101 gatgtatatt attgccgaag gaccggaagg tatgtacttg atcgatcagc acgcggccca
     2161 cgaacgcgtg ctgtatgaaa aatttatggc cgataaaacg tcgcagcata tcgcggtgca
     2221 gaatctgctc gatccgctgc caatggaatt ttcgcctgat gaatcgacat tgatcgaaga
     2281 gaacatcgag acgttgcata cgattggatt gcacctcgat gcattcggcg gatcgacctg
     2341 gttgattcgc agtattccat cgttgttggc gaaggatgac att
//
LOCUS       DASRTJ010000481         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_278098,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000481 DASRTJ010000000
VERSION     DASRTJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278098"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="VFF70_09820"
     CDS             <1..1631
                     /locus_tag="VFF70_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45033.1"
                     /translation="PLMLCLGFEFLWRGLTAEIRSQKPEARSPAISLSRRLAISPSLR
                     LFIFGGIFLGLSAYTYLSSRAIPILLIAFVIYLALFARDRMRGRWRNLFICFAIAIVV
                     ALPLAITVLTNPDLQFRVSEVSDPLDQALKGDTQAIMANIPLAFGMFTDRGDNTVRDN
                     LPDRPVFPEPIGQALLIFGFVIALSKFNQPRYALALIWLGSMLVPSIVTSGAPNFTRT
                     LGALPMAFALPAIGLEWLSMWPLRMIAMPHQQKLSRIFVPIAIVFLIGVFAINAASTF
                     DDYFNKWPNHSETQFVFQADFAAIAKDIDANNVMDVSVGGLSNDTLDDDSLRLLLQRK
                     DVRVRWFDSGSPISSGGAIVEGYNKALPIYIPSMVPVSSLFSIYLDDNRCLELFVDNH
                     FRHYQLRCEGVGGISPKARSFNDTALLQDTPQNFPDTILAGDRFQFTTTWIAQFPIDY
                     PRRVFVHLVDPRNGLIVAQHDGLDAPTKFWGDFDQIIQVHTLTVPLETVPGIYEIWIG
                     LYDPSTGQRVLQTDSAHSEPPSDQYLLGTIQVAR"
     gene            1697..>1890
                     /locus_tag="VFF70_09825"
     CDS             1697..>1890
                     /locus_tag="VFF70_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45034.1"
                     /translation="MRSFSLPRFLAIVLLLILTWLQFSTLSITSPTMDEPSHIVSGYA
                     FLTRGDTRIKLNGPILPNLI"
BASE COUNT          411 a          533 c          431 g          515 t
ORIGIN      
        1 tgccgttgat gctgtgtctg ggctttgagt ttttgtggag aggattgacc gcagaaatta
       61 gaagtcagaa gccagaagcc agaagtcccg ccatctcgct gtctcgccgt ctcgctattt
      121 ctccttctct acgtctattc atctttggtg gcatcttcct cggtctatct gcctacactt
      181 atctctcctc tcgcgcgatt ccgatcttgt tgatcgcctt cgtgatctat cttgcgctct
      241 tcgcgcgcga tcgaatgcgc ggccgatggc gcaatttgtt catctgcttc gcaattgcga
      301 tcgtcgttgc gttgccatta gcgatcaccg ttctgactaa tcctgatctg caattccgcg
      361 tctccgaagt cagcgacccg ctcgatcaag cattgaaggg cgacacacaa gcgatcatgg
      421 ctaacatccc gctcgcattc ggaatgttca ccgatcgagg cgacaacacc gtgcgcgaca
      481 acttgccgga tcgacctgtt ttcccggagc cgatcggtca ggcgttattg atcttcggat
      541 ttgtcatcgc gctatcgaag ttcaatcagc cgcgctatgc gttggctttg atctggctcg
      601 gatcgatgct ggtgccatcg atcgtcacaa gtggcgcgcc gaactttacg cgtacgctcg
      661 gcgcactacc gatggccttc gccttgccag caatcggcct cgaatggctt tcgatgtggc
      721 cgctgcgcat gatcgccatg ccacatcaac aaaaattgtc gcgcatcttc gtgccgatcg
      781 cgattgtatt tttgatcggt gtgtttgcaa tcaatgctgc ttcgacattc gatgattact
      841 tcaacaagtg gccaaatcat tctgaaaccc aatttgtttt tcaagccgac ttcgccgcga
      901 ttgcgaaaga catcgatgcg aacaatgtga tggatgtatc ggtcggcggc ctatcgaacg
      961 atacgctgga tgatgacagt ctgcgcttgc tacttcaacg caaagacgtg cgcgtccgct
     1021 ggttcgattc ggggagtccg atcagcagtg ggggagcgat cgtcgaagga tataataagg
     1081 cattgccaat atatattcct tcaatggtcc ctgtttcatc tctattttca atctatttgg
     1141 atgacaacag atgccttgag ttatttgtgg ataaccattt tcgtcactat cagttgagat
     1201 gtgaaggcgt tggtggtatt agtcccaaag cacgatcatt caacgacact gcactgcttc
     1261 aagatacacc tcaaaacttc cctgacacga tattagccgg tgaccgcttt cagttcacaa
     1321 ccacatggat agcacaattc cccatcgact atccacgtcg agtattcgtt cacttggtag
     1381 acccccgcaa cgggctaata gtcgcgcagc atgatggtct cgacgcgcca acaaaattct
     1441 ggggcgactt cgatcaaatc attcaagttc acacgctaac tgtaccgctt gaaactgttc
     1501 ccggcattta tgaaatctgg atcggcttgt atgatccatc gaccggacaa cgagtcttgc
     1561 aaaccgactc tgctcattcc gaaccgccga gcgatcaata tctgctcggc acaattcagg
     1621 tagcgcgata attcgcgcat cgctcttacg cattactcat tatttttcac gcatcactcg
     1681 tcactaatca ctcatcatgc gctccttctc cctacctcgc tttctcgcca tcgtcttgct
     1741 gctcattctt acctggctgc aattcagcac tctatcgatc acttcgccga cgatggatga
     1801 gccaagccac atcgtcagcg gttacgcctt tctcacacgc ggcgatacac gcatcaaatt
     1861 gaacgggccg atcctgccga atttgatcag
//
LOCUS       DASRTJ010000482         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_202119,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000482 DASRTJ010000000
VERSION     DASRTJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202119"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1280
                     /locus_tag="VFF70_09830"
     CDS             243..1280
                     /locus_tag="VFF70_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZY45035.1"
                     /translation="MSTAKPVYIGTDGGATTSKVSGVWSDGTPVSISLLQRSTNSQLG
                     PEAVVGSWVEAITEYLEQNELTWDQVQGVGLSIPGVFRRYGVLDRSANFPAVFHGWEV
                     HAAYSGVLAERAGRAVPLVVGNDGNYGGVAEAQRVRGAGRGSVLMLAPGSGLGCAYID
                     RNGLPFDGDTLAGMEGGHMPAPLHLLNAKAYPCGCGRTWGCVEVYTTISGLPYLLAEK
                     LIEYPDHALAKSPLSPKEKALALRGLAQQGDRLAVEIFDFQARAMGLHVANLVMALDP
                     QFVVIGGGLMDPEATTEAFRERYLRIVRETAAPYFWPAQRDHVSIVPAALGDLSQAIG
                     AALVALYQSRS"
     gene            1354..>2060
                     /locus_tag="VFF70_09835"
     CDS             1354..>2060
                     /locus_tag="VFF70_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45036.1"
                     /translation="MKRISIFLLLLTVIWLAGCGAPASTLTPLSVTQLKYILMDYFGG
                     MAVDHGILYCDPDRYPLQIDDQEHKNALEKFMQIAQDADRFQMIVGHIGLANLSEYTD
                     EQKLLIYRQDKQLNAFILTAIDHGYSFDLTLVGGPGIFTEVKGTISEQGAVQITSQAK
                     LSTPGCPICLLPDTKIDTPDGQIAVKDLIVGTPVWTVNHVGMRVTGVVVETVKRPVPP
                     NYPIVHLRLKDGRELFIS"
BASE COUNT          458 a          495 c          591 g          516 t
ORIGIN      
        1 agtccgaagc cagaagcata atcaaatgaa ggggcggggt gacctcgcct ttactaaccg
       61 cagaggcgcg gagacgcaga gatttcaaat gcggctccgc gcttttttgc gcctgacagt
      121 tgtcgaatca gcttgatgcg cgtatacttc gggtctgtcc gatcgattgg agaccggcat
      181 cttccttgat gcagccatgt ctatcggata acggttggtc gaaaaatttg gcaggagcca
      241 acatgagcac agcgaaacct gtttacatcg ggaccgatgg tggcgccacc acctccaagg
      301 tgagtggcgt ctggagtgat ggcacgcccg tatcgatcag cttgcttcaa cgatcgacta
      361 attctcaact cggccccgag gccgtcgtcg gcagctgggt cgaagcgatt accgaatatc
      421 tggaacaaaa tgagctgact tgggatcaag tacagggcgt ggggctgtcg attccaggcg
      481 tatttcggcg ttatggtgta ctcgatcggt ctgcaaattt cccggcggtt ttccatggtt
      541 gggaagttca cgcggcttac agcggcgtat tggcagaacg ggccggacga gccgtgccgt
      601 tggtcgtcgg caacgatggt aattatggcg gcgtggccga ggcgcagcgt gtgcgcggtg
      661 cgggtcgagg cagtgtgctg atgcttgcgc cgggatcggg actgggttgt gcctatatcg
      721 atcgcaacgg cctgccgttc gatggtgaca cgctggccgg aatggaaggc ggccacatgc
      781 ctgcgccgct gcatctgttg aatgccaaag cttatccatg tggttgcggt cggacgtggg
      841 gctgtgttga agtttacacg acgatctccg gtctgcctta tctgctcgcc gaaaaactga
      901 tcgagtatcc cgatcacgct ctggctaaat cgccgttgag tccgaaagag aaagctttgg
      961 ccttacgtgg tttggcccag caaggcgatc gcttggcggt cgaaatcttc gactttcagg
     1021 cacgtgcgat gggcttgcat gtcgccaacc tcgttatggc gcttgatccg cagtttgtcg
     1081 tgatcggtgg cggactgatg gatcccgaag cgacgaccga agcatttcga gagcgctacc
     1141 tgcggatcgt ccgcgagacg gcggctccct atttctggcc ggcgcagcgc gatcatgtca
     1201 gcattgtccc ggccgcgttg ggcgatctat ctcaagcgat tggcgcggca ttagtggcct
     1261 tgtatcaaag tcgatcgtag tggattgacg attgtagggg gtgaggtcac ctcgccctac
     1321 taacggcaga ttgggtaatt tcattatcta atcatgaaga gaatctcgat tttccttttg
     1381 ttgctgacag ttatttggct cgccggatgt ggtgccccgg ccagcacgct aactccttta
     1441 tcagtcactc aactcaaata tatcctgatg gattattttg gcggcatggc tgtagatcac
     1501 ggaattctat actgtgatcc agatcgttat ccgctacaaa ttgatgatca agaacacaag
     1561 aatgcacttg agaagttcat gcaaattgcc caagatgccg atcggtttca aatgatagta
     1621 ggccatatcg gcttagcaaa tctttctgag tatacagacg aacagaagct gcttatctat
     1681 cgacaagaca aacaactaaa tgcattcatc cttacagcta ttgatcatgg ttacagtttc
     1741 gatttgacgc tggtgggcgg gccgggtatt tttacggaag ttaaaggaac aatttctgag
     1801 caaggtgcag tccagattac tagccaggct aagttatcaa cgccgggatg tccgatatgt
     1861 ttgttgcctg atacaaagat cgatacacct gatggtcaaa ttgcagttaa agacttgata
     1921 gtgggtacac ctgtttggac agtgaatcac gttggaatgc gggtgactgg cgttgtcgtt
     1981 gaaacagtga aacgccctgt cccgcccaac taccctattg tgcacttgcg cttgaaagat
     2041 ggccgcgaac tatttatatc
//
LOCUS       DASRTJ010000483         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_333479,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000483 DASRTJ010000000
VERSION     DASRTJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333479"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFF70_09840"
     CDS             <1..257
                     /locus_tag="VFF70_09840"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZY45037.1"
                     /translation="KVYLGWSTIYFTLTGRTKDLIIAIGPMPDRLNLYFARGVDLDDP
                     NELLEGTGKKMRHVKIFESKQIRSRALKALIKQAVTIAKA"
     gene            355..1641
                     /locus_tag="VFF70_09845"
     CDS             355..1641
                     /locus_tag="VFF70_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZY45038.1"
                     /translation="MTIPTISSLTARRYILGKQGLWPGRRWIGQAGVAQALKEIEAIQ
                     IDPLNVVGRSHDLALHSRVIDYQPQQFENVLYNDRLGFDWGGALFVYPMAELPYWRAV
                     MKRLKDHPQWGIFPQGQRALINEVKRELRQRGPLGNRDFDGRRRVDHYRASKDSGLAL
                     YYLWRSGELMTHSRRGNFERVFGFRDHIAPPEMNRVASIKETEQFFARKTIAFYGLMT
                     ARSWRLGFANKIDRTVTLAEAQRRLDRAIKSKEFSLVNVEGWKEPAYLLAEDQDLLLD
                     LETGQIPDRWRPIDTTTDEEILFLAPLEIVSARGRAKTLFDDYEYIWEVYKPEPQRRW
                     GYYTLPILYGDRLVARIDPRHAKASNTLVINGLWLDDRLLAKDATFMQALLRGISRLL
                     KYLNTEAIDLSPLQAKVKGKSDLEAQWIRSVVKRLS"
     gene            1682..>1892
                     /locus_tag="VFF70_09850"
     CDS             1682..>1892
                     /locus_tag="VFF70_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZY45039.1"
                     /translation="MKTNQAAPQTIDQYIADFPEDIQKILQKIRATIRKAAPGAAEAI
                     KYQMPTFVLNGNLVHFAAFKHHIGFY"
BASE COUNT          482 a          488 c          509 g          413 t
ORIGIN      
        1 aaaaggtgta tctgggctgg agcacgatct atttcacttt aaccggcaga accaaagatc
       61 tgatcattgc gatcgggccg atgcctgatc gattgaattt atatttcgcg cgcggcgtcg
      121 atctggacga tccgaatgaa ttgcttgagg gcacgggcaa aaagatgcgg catgtgaaaa
      181 tttttgaatc gaagcaaatt cgatcgagag cgttgaaggc attgatcaag caagcagtaa
      241 cgatcgctaa agcgtgatcg tcattgcgag cgaagcgaag caatctcacc ttcggtcgat
      301 caggagattg cttcgtcggc ctactggcct cctcgcaatg acggctgaac catcatgacc
      361 ataccgacta tttcctctct cactgcccgc cgatatattc tcggcaagca ggggctgtgg
      421 cccggccggc gctggatcgg ccaagcgggc gtggctcagg cactgaaaga gatcgaagcg
      481 atccagatcg atccactcaa cgtcgtcggc cgcagccacg acctcgcgct gcacagccgc
      541 gtgatcgatt atcaaccgca gcaatttgaa aatgttttat acaatgatcg cttgggtttc
      601 gactggggcg gcgcgttgtt tgtgtatccg atggctgagc tgccctattg gcgcgccgtg
      661 atgaaacgcc ttaaagatca tccgcagtgg ggtatttttc cacaaggaca gagagcgtta
      721 atcaacgagg tcaaacgcga gctgcgccag cgcggcccgt tgggcaatcg agatttcgat
      781 ggccgccgcc gcgtcgatca ttatcgcgcc agcaaagatt ccggcctcgc cttgtattac
      841 ttgtggcgca gcggtgagct aatgacgcat tcacggcgcg gcaacttcga gcgcgtgttc
      901 ggttttcgcg atcacattgc accgccagaa atgaatcgcg ttgcatcgat caaggagacg
      961 gagcaattct tcgcgcgcaa gacgattgca ttctatggat tgatgaccgc gcgatcgtgg
     1021 cgcttgggct ttgccaacaa gatcgatcgg acagtcacgc tcgctgaagc ccaacgccgg
     1081 ctcgatcgag cgatcaagtc caaggagttt tcgctggtga atgtcgaggg ctggaaagaa
     1141 ccggcttatt tattagcgga agatcaagac ttgttgctgg atcttgaaac gggacaaatt
     1201 cctgatcgat ggcggccgat cgacacgacc acggacgaag aaatcttatt cctcgcgcca
     1261 ctggaaatcg tcagcgcgcg cggccgcgcc aagacattat ttgatgacta tgaatatatc
     1321 tgggaagtgt ataagcccga gcctcaacgc cgctggggtt attacaccct gccgatcctg
     1381 tacggcgatc gattagtcgc ccggatcgat ccgcgccacg ccaaggccag caacacgtta
     1441 gtgatcaacg gcctgtggct cgacgatcga ttgctggcaa aagatgcaac cttcatgcaa
     1501 gcgctgttgc gcggcataag tcgcctactg aaatatctca acactgaagc gatcgatctg
     1561 tcgccgctgc aagccaaggt gaaaggcaaa agcgatctcg aagcgcagtg gatccggtcg
     1621 gttgtaaaac gattgagtta aaattcgatc aagaattaac gcagtcgacc gaggagacaa
     1681 gatgaaaact aatcaagcag caccccaaac tatcgatcaa tatattgcgg atttcccgga
     1741 agatattcaa aaaattctgc aaaagatacg ggcgacaatt agaaaagcgg caccaggcgc
     1801 ggcagaagcc atcaagtatc aaatgccaac cttcgtgcta aacggcaatt tggttcactt
     1861 tgccgccttc aaacatcata tcggttttta tc
//
LOCUS       DASRTJ010000484         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_105729,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000484 DASRTJ010000000
VERSION     DASRTJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105729"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VFF70_09855"
     CDS             <1..1831
                     /locus_tag="VFF70_09855"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZY45040.1"
                     /translation="VGLLLALGGYTPIYYALLKIFPGFDLFRAPARWIVLFAFAGSIM
                     TGVGLDSLRDRKINFRSLLLPIVFVGLLVLLTFISGGLTPAGASGPIGIPDVASLLLW
                     IVAFVITILFIKISSFILHPSSFITHYSLLITLSCLELFLATRTLPYNSHATAPDALT
                     DLRSATAHLLVGAQDRTPPDRFLSISDIFFDPGDSAELKSIFGNQLSNDAYYDLIIAT
                     KFKEIVAPNLPLYYRLTAVDGYDGGLLPLKNYMTFQQLFLDPSLIQSDGRLREQLKSI
                     PAGRWLDLMNVHYIITDKVGDQWYDGVLYDLQFSTTLISGQSASTSQLPSFQADALGL
                     VYSASISNTTLASIDLKFADGTTQTLMLNDSCEGSSSSQDLTTCRLSWIGSKRVISIK
                     VTGVGGITLHGAALIDQTSGAFQSFVIAPHGEFKLAHSGDVKVYENVDALPRAFFVSN
                     EQTVANDEEAIALMKSDQFNPSQAVVLIGNQNTNSTDHLVPLSPSPLVPPSPSHLVTY
                     EPEHIIVDVDAQQDGYLVLTDAYYPGWVATLDDRAAEIDRADVLFRAVKVPAGQHRID
                     MRYDPQSYKIGSIVSIGAWIVWLMLFVVTRRRVIIAQPSMTNN"
     gene            1838..2476
                     /gene="scpB"
                     /locus_tag="VFF70_09860"
     CDS             1838..2476
                     /gene="scpB"
                     /locus_tag="VFF70_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZY45041.1"
                     /translation="MTKSENKSHEVELPKELPLPLTAMIESLLFVSSEPVQINYLAEA
                     LNTPIDHIEQSLAELKDGSMGRGLRLQRKGDRVQLTTRPEVAPYIEKFLGLDLSSRLS
                     KPALETLAIVAYQQPATRPQIEQVRGVNCDAVLSTLLTKGLIEEIGRLETAGRPIQYG
                     TTFAFLQHFGLRGLDDMPQLKPGEAATLEAMATNKNDQMTNDQITNGPVVNG"
     gene            2469..3338
                     /locus_tag="VFF70_09865"
     CDS             2469..3338
                     /locus_tag="VFF70_09865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZY45042.1"
                     /translation="MAEERIQKLLALAGLGSRRSCEDYLVQQRVKVNGKLVELGAKAD
                     PDRDTITVDDRRVQFESKKIYVMLNKPLGVITTNEDEFDRKTVRDLIPIDGHLYPVGR
                     LDADSEGLVLLTNDGDLANALTHPRYEHEKEYMVLVDGRPEERVLNSWRRGVMWDDQK
                     TAPARVEVIDTQNSQTWLRIIMHEGRKRQIREIGGMLGHRVKHLQRVRIGPLLLGDLK
                     SGSWRYLTASEISQLRAIVDQAASRKPVKEGRPRKLIKRSDGPTKSRTRSDQRGTDRS
                     SGAMRGVKQTNRK"
BASE COUNT          770 a          870 c          907 g          800 t
ORIGIN      
        1 cgtcggtttg ttgctcgcgc tcggcggtta cacaccgatc tattacgcgc tgctaaaaat
       61 ctttcccggc ttcgatctct ttcgtgcgcc tgcgcgctgg attgttttgt ttgccttcgc
      121 tggatcgatc atgacgggcg ttggcctaga ctctctgcgc gatcgaaaga tcaactttag
      181 atcattgctg cttccgattg tttttgtcgg cctgctcgtc cttctgactt ttatatctgg
      241 cggcttaaca cccgccggcg cttccggccc gatcggaatt ccagatgttg cctctctctt
      301 gttgtggatc gttgcctttg taataacaat tctattcatc aaaatttcat ccttcatcct
      361 tcatccttca tccttcatta ctcattactc gttactcatt accctctcct gtctcgaact
      421 ctttttggca acccgcactc ttccatataa ctcacatgct actgcgccgg acgcgttgac
      481 tgatctccga tcggctactg cacatttgtt ggtcggcgcg caagatcgaa cgccgccgga
      541 tcgattcttg tcgatctcgg acatcttctt tgatccgggc gacagcgccg aactcaaatc
      601 gatctttggt aatcaacttt cgaacgatgc ttactatgat ctgatcatcg ccaccaaatt
      661 taaagagatc gtcgcgccga acttgccttt gtactatcgc ctgactgcgg tcgatggcta
      721 cgacggtggc ttgctgccct taaagaacta catgaccttc cagcagttgt tcctcgatcc
      781 gagtttgatt caaagcgatg gccgcttgcg tgaacaactc aaatcgattc ccgctggacg
      841 ctggctcgat ctgatgaacg tgcactacat catcaccgac aaggttggcg atcaatggta
      901 tgacggggtc ttgtatgatt tgcaattctc aacaacgctc atttctggac aaagcgcttc
      961 gacttcacaa cttccatcgt ttcaagccga tgcgttaggc ttagtttatt ctgcgtcaat
     1021 tagcaacaca actttggctt cgatcgatct gaaatttgct gacggcacaa cacagacatt
     1081 gatgttgaat gattcgtgtg aaggctctag ttcttcgcaa gacttaacaa cctgtcgttt
     1141 gagttggatt ggctcgaagc gcgttatctc aataaaggtg accggtgttg gcggcattac
     1201 attgcatggt gcggcgttga tcgatcaaac gagtggggcg tttcaatcat tcgtgattgc
     1261 gccgcatggc gaattcaagt tggcacattc gggcgatgtg aaagtgtatg agaatgtcga
     1321 tgcgctgccg cgcgcgttct tcgtaagcaa tgaacaaact gtggcaaatg atgaagaggc
     1381 catcgcctta atgaagagcg atcaattcaa tccctcacag gctgtggttc tcatcggaaa
     1441 ccagaatact aattcaacag atcaccttgt ccccttgtct cccagtcccc ttgtccctcc
     1501 ttcccccagt caccttgtca catatgaacc tgaacatatc atcgtcgatg tagacgctca
     1561 acaagacggc tatctcgtcc tcaccgacgc ttactacccc ggctgggtcg ccacactgga
     1621 cgatcgggcc gccgagatcg atcgggcgga tgtgttgttc cgcgcggtga aagttccggc
     1681 tggccagcat cgaattgaca tgcgctacga tccgcaatcg tacaaaatcg gatcgatcgt
     1741 ttcgatcgga gcatggatcg tgtggctcat gttgttcgtc gtcactcgcc ggcgtgttat
     1801 aatcgcgcag ccatccatga caaacaatta atgcccaatg acaaaatcag aaaacaaaag
     1861 ccacgaagtt gaactgccca aagaattgcc gctgccgctg acggcgatga tcgagagctt
     1921 gttgtttgtg tcaagcgagc cggtgcaaat caactacctc gcggaagcgc tgaacacgcc
     1981 gatcgatcac atcgagcagt cgctggctga actcaaagac ggatcgatgg ggcggggctt
     2041 gcggttgcag cgaaaaggcg atcgggtgca gctgaccacg cgacccgaag ttgcgccata
     2101 tatcgaaaaa tttctcggcc tcgatctgag cagccgctta tcgaaaccgg cgctggagac
     2161 gctggcgatc gttgcatatc aacagccggc gacgcgcccg cagatcgaac aggtgcgtgg
     2221 cgtgaattgc gatgcggtgc tctcgacgct gttgaccaag ggactgatcg aggagatcgg
     2281 ccgattggaa acggctggcc gtccgattca atatggcacg acgttcgcat tcttgcagca
     2341 ttttggtttg cgcggtctgg atgatatgcc gcagttgaaa ccgggcgagg cggccacgct
     2401 cgaagcgatg gcgacgaata agaatgatca aatgacaaat gaccaaataa caaatggacc
     2461 ggtggtgaat ggctgaagaa cgaattcaaa aactgttggc actggcaggt ttaggctcgc
     2521 ggcgatcgtg cgaagactac ttggtgcagc aacgcgtcaa agtgaatggc aaactcgtcg
     2581 agttaggtgc gaaagccgat ccggatcgtg acacaattac ggtcgatgat cggcgtgttc
     2641 aatttgaatc gaagaagatc tatgtgatgc tgaacaagcc gctcggcgtc atcacgacga
     2701 acgaagacga gttcgatcgc aagacggtgc gcgatctgat cccgatcgat gggcatctat
     2761 atcccgtcgg tcgattggat gcggatagtg aaggcttggt gctgctcacg aacgatggcg
     2821 atctagcgaa tgcgttgacg catccgcgct acgagcatga gaaggaatac atggtgctgg
     2881 tcgatggccg gcccgaagag cgcgtcttga atagttggcg gcgcggcgtg atgtgggacg
     2941 atcaaaagac ggcgcccgcg cgcgtcgaag tcatcgacac acaaaacagc cagacgtggc
     3001 tgcgcatcat catgcacgaa ggccgcaagc ggcagattcg agagatcggc ggcatgctcg
     3061 ggcatcgcgt caagcatttg cagcgcgtgc ggatcgggcc gctgctgttg ggcgatttga
     3121 aatccggcag ctggcgatat ttgactgcga gtgagatcag tcaactgcgg gcgatcgtgg
     3181 atcaggcggc gagtcgtaaa cctgttaaag aaggccgacc acgcaagctc atcaaaagat
     3241 ctgacgggcc gacgaaatcg cggacgcgat cagatcagcg cggcaccgat cgatcttcgg
     3301 gcgcgatgag gggcgtgaag cagacgaatc ggaagtaggg gttgggc
//
LOCUS       DASRTJ010000485         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_258041,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000485 DASRTJ010000000
VERSION     DASRTJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258041"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..870)
                     /locus_tag="VFF70_09870"
     CDS             complement(202..870)
                     /locus_tag="VFF70_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZY45043.1"
                     /translation="MFVIIVGGGKTGSQVASELLKAGHQVRIVEDRSAVLERLKHELP
                     AASVVAGDGSTPSVLEAAGVHEAQVLAAVTGADETNLVVTTLGRFEFNVPRIIARVNN
                     PKNGWMFTPEMGVDVALNQADLMAHLILEEMSLGDMTTLLKLRKGQYSLVEEKVHPTA
                     LANGKAIRNLLLPAECVLVAVIRAGQLIIPHGDVVLQSADEVLALVHSSQMSALAELL
                     NRTG"
     gene            complement(873..1532)
                     /locus_tag="VFF70_09875"
     CDS             complement(873..1532)
                     /locus_tag="VFF70_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZY45044.1"
                     /translation="MKVIIMGCGRVGEQVSLLLDADGHDVSVIDYNAEALARLGPRFK
                     GKTVKGIGFDRNVLMEAGIEQADAFVATSSSDNANVVAARLARNIFQVPRVVARLYDP
                     QRAEVYHRLGLVTISSTTWGAQRIQELLTHADLDPVLTFGRGEVVLIAIEAPSHLVGR
                     TVNNVTVSGEASVVSITRKDQAIIPTLGTEFRSGDLIHLAVLATAMERIEDLLGLGEG
                     G"
BASE COUNT          395 a          550 c          434 g          383 t
ORIGIN      
        1 gtatcaagct catcattttg attttgcgcc aacgattact agacctaaat gtggtgattg
       61 attacatcgc aggcaggatc gatccgtgaa gagcagctgt tcgaatgtga gggcgcggac
      121 cgtgacccat cataaataaa acacgccaag gaaatttagc cctggcgtgc ccttaaatgg
      181 ttggagtcgc gttctcccgc tctaaccggt ccggttcaac agctccgcca acgccgacat
      241 ctgcgaacta tgcaccagcg ccaaaacctc atccgccgac tgcaacacca catcaccatg
      301 cggaatgatc agctgcccgg cgcgaatgac ggccaccagc acgcattctg cgggcagcag
      361 caaatttcgg atcgccttgc cgtttgccag cgccgtcgga tgaactttct cttcgacgag
      421 tgaatactgt cccttgcgca gcttaagcag tgtcgtcata tcacccaatg acatctcttc
      481 caggatcaaa tgggccatca agtccgcttg atttagcgcc acatccacgc ccatttccgg
      541 ggtaaacatc cagccgttct tgggattatt gacccgcgca atgatgcgtg gcacgttgaa
      601 ttcaaatcgg cccagtgtgg tgacgactaa attcgtttcg tctgcaccgg tcaccgcggc
      661 cagcacttgc gcctcatgca cacccgcggc ttccaacacg ctcggcgtgc tgccatcgcc
      721 ggcgaccacg gaagccgcgg gcagttcatg tttcaatcgc tccagcaccg ccgatcgatc
      781 ttcgacgatt ctgacttgat ggcctgcttt caacaactcc gatgccactt gcgatccggt
      841 ttttccgccg ccgacgataa tcacaaacat ggctagcctc cttcacctaa acctaataga
      901 tcttcgatgc gttccatcgc cgtagccagc acagccagat ggatcagatc gcccgatcgg
      961 aattctgtgc ccaacgtggg gatgatcgct tgatctttgc gcgtgatcga cacgacactc
     1021 gcttcacctg acacggtcac attgttgacg gtgcggccaa ccaaatgcga cggcgcttca
     1081 atcgcgatca acaccacctc gccgcgcccg aaggtcagca ccggatcgag atcggcatgc
     1141 gtcaacagtt cttgaatccg ctgcgcgccc cacgtcgtgg aggagatcgt caccaagccc
     1201 aaacggtgat agacttccgc gcgctgcgga tcatacagac gcgcgacgac gcgcggcact
     1261 tgaaagatat tgcgagccag ccgcgccgcc acaacgttgg cattgtccga cgagctggta
     1321 gcgacgaagg catcggcctg ctcaatgccg gcttccatca gcacattccg atcgaagccg
     1381 atgcccttca cggtcttgcc tttgaagcgc ggtcctaacc gggccaaggc ttccgcatta
     1441 taatcaatca ccgacacgtc gtgtccgtcg gcatccagta acaggctgac ctgttcaccg
     1501 actctgccac aacccatgat aatgactttc atcgtaacct cctgaaggga gaatcatttc
     1561 gaatattttg cttggtattc taagacaact tcacccgagc gtgatgttgc gtagcaaaat
     1621 ctacaacggc ggctgccacg ccgccggatt cgggcgtggg agcccgaacc gctgagtttc
     1681 taatcacctg gattcgatcg tacctcaatt tcagatcgag cagggttaca tgtacgttgc
     1741 gcctgctcgc tgcctgaaac gc
//
LOCUS       DASRTJ010000486         7483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_258090,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000486 DASRTJ010000000
VERSION     DASRTJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258090"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFF70_09880"
     CDS             <1..420
                     /locus_tag="VFF70_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZY45045.1"
                     /translation="REDELFDAEGAVKESRYSLTYTQPEKEREFTHSVLLSRSRFTAR
                     ADRSLRFYREYFRPLQEREVIKERRRYHIVYKNEEFAVNIDRIVSPEMPGWYVEIKSR
                     TWSPRDAEAKAALIAEMLRLLGVEPDKVMRTEYVEQL"
     gene            complement(728..1669)
                     /locus_tag="VFF70_09885"
     CDS             complement(728..1669)
                     /locus_tag="VFF70_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45046.1"
                     /translation="MKRISFLILVVALFVSACGGAQPNLPDTNALSTQIAATVNAAIN
                     NALPTSVPNIVPTKAPIVPPPAATQTPAPTKAPLPNGSGGSFSPITFAIGENHEQPVD
                     AMDTFPGGVTMIYAIFSGVNVKNGDAWRNEWYLNGKLQDKLSASDNWDAATAGPDSVW
                     WLTVFNPDGVRSGQWELKLYVGENLVQSGQVTVEANVEPDFGPITFAGGVDSSDQPVN
                     PVEVSDPTFPAGTKEVYAFFSGINMPKDTAWQTQWFLNGEPADSKDHTWNFGLTEEDW
                     IQFGSTGSSPLDPGTYELKLSIDGAIVNIGSFVVSAN"
     gene            complement(1769..1903)
                     /locus_tag="VFF70_09890"
     CDS             complement(1769..1903)
                     /locus_tag="VFF70_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45047.1"
                     /translation="MAWFIGLAPADLPSYAIAVLIEVPSGQRIDRAEQIVRATLIDLL
                     "
     gene            complement(2015..2938)
                     /locus_tag="VFF70_09895"
     CDS             complement(2015..2938)
                     /locus_tag="VFF70_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45048.1"
                     /translation="MKKIGLLIIVVAMFVSACGGAQPNLPDTNALSTQIAATVKAAFN
                     SVTTSVPQLAATAKPIVPPAATQAPANPPPNSTASFSPITFAIGVENEQPVDAMDTFP
                     SGVTKIYGVFSGINIKDGQAWRHEWLLNGESFPELSSGTTWKAAIAGVNSVWWITPSF
                     PEGMDAGEWELRLYIGDQLVQSGKVTVEANVEPDFGPITFAGGVDTNDQPVDPVTVRK
                     PTFAAGTKEVYAFFSGINVTADTDWQSEWFFNGKSLDSKNHAWNLSPNEENWIQFGST
                     SSSPLTPGTYELKLTIDGTVVNIGSFVVLAN"
     gene            complement(3027..4364)
                     /locus_tag="VFF70_09900"
     CDS             complement(3027..4364)
                     /locus_tag="VFF70_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZY45049.1"
                     /translation="MTKSIQRISLAFLAAFGVLALALGIWSIGSPGLVERDDNARLII
                     AEQRIQRGAIIDRYDRILAETIPVSGTFVRHYPDRSAVPAVGYYSINFGRSEIEEALD
                     QTLRGPYGFLDELLHRERAGRSVRLTIDLAEQRALTDQFDRPGGAVVVSIPDGAIIAL
                     ASYPSFDPNSLDVNWKTLSSDSASPLLNRATQGLFQPGAIFETPLLADVIDRGFVSIT
                     QTLTRPDQSIAIGSLVLNCYRSEATPTLAEAYANACPASFADLRIALGETELISITEH
                     WHLDVPPSLEIRTAAALTATVALTSTEALNAFATGQSQLTISPLHMALVAATIGNHGN
                     MPQVHLVQDFQSIDGEWQPYHSDSATIPAERIISNSTAEIVLAAMRTIDNTAGHSGIA
                     FSGSSQLAWFIGLAPADRPRYAIAVLIEVPSGQRIDRAEQIGRAALIDLIKSR"
     gene            complement(4375..5754)
                     /locus_tag="VFF70_09905"
     CDS             complement(4375..5754)
                     /locus_tag="VFF70_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZY45050.1"
                     /translation="MSLQSALRTPQSALENPQLNNTQPIETAYLWSRLPINILRERVL
                     LVLAGAFTLSGFVLLELISNQEHLTFFIVNWSVLIAFLVWSICFTAIHLHFNRRVRGR
                     DPFLLPIAALLTGWGLIEVARLAPGYLSRQVIWLIVGSSALIAITWSPANLKWLRRYR
                     YTWLIGGLFLLALTFVIGVNPEGSGLRLWLGGLAGVFFQPSEMLKLLFVAFLASYLAE
                     KREIAQSLQIEHQRFLTAPYLAPLIAMWAVALILLIGQQDLGTAILLYLTFLVMLYLA
                     SSQPRYLLVGVLLLIVAGLIGFQLIGRVRGRVSTWIDPWTDSAGSSYQIVQSLIALGS
                     GGAIGSGLGQGSPDIILPAAHTDMPLAAIGEEFGLIGTLAVVICFSLLTLRGLRAALR
                     ARTTYGQLLAAGLATSIGVQAWIIMAGNANVAPLTGVTLPFVSYGGSSLLVSFLAIGL
                     LSQISADRD"
     gene            complement(5764..6705)
                     /gene="mtnP"
                     /locus_tag="VFF70_09910"
     CDS             complement(5764..6705)
                     /gene="mtnP"
                     /locus_tag="VFF70_09910"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZY45051.1"
                     /translation="MLNIEKVKLAIIGGSGLYDMEALTDVKEFKIKTPFGDPSDAIVV
                     GSLSSQRVAFLPRHGRGHRLTPTEVNSRANIWALKSIGVERIVSVSACGSLREEFEPR
                     HIVIPSQLFDRISGRPLTFFGNGIVAHIGLADPFCPELSGLLYEAVKNTGATVHMGGW
                     FVTIEGPRFSTKAESTLFRQWGMDIIGMTAIPEANLAREAEICYACMAHVTDYDVWHA
                     GEETVNVQMIIERLLANVEVSKRAIAYLAPAIPNRRTCECPTALANAIITQPQFVPLK
                     TKRDLAPIIGKYMPIAATKAAKKVVKKKLPRRRVTRG"
     gene            6845..7387
                     /locus_tag="VFF70_09915"
     CDS             6845..7387
                     /locus_tag="VFF70_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HZY45052.1"
                     /translation="MNKPAGLPTLVDGYNKDAPSLVGLVQQRYGRVWIVHRLDKDTSG
                     VVLFARSADVHRSLNIAFETRHVSKVYHAIVNGVPEWNKLEIEFPLKADGDRRHRTVV
                     DRAEGKAALTRCRVLEKFTAHALIEAKPETGRTHQIRAHLAAQGFPIVGDELYGRPAR
                     VIDRTLIDRSALHARSITIA"
     gene            complement(7396..7467)
                     /locus_tag="VFF70_09920"
                     /pseudo
     CDS             complement(7396..7467)
                     /locus_tag="VFF70_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1753 a         2023 c         2021 g         1686 t
ORIGIN      
        1 cgcgaagatg aactgttcga cgcagaaggt gcggtcaaag agtcgcgcta ttcgttgacc
       61 tatactcaac cggagaagga acgcgagttc actcattccg tgctgctgtc gcgttcgcgc
      121 tttaccgcgc gagctgatcg gtcgttgcgg ttctatcggg aatatttccg gccgctgcaa
      181 gaacgcgagg ttatcaaaga gcggcggcgg tatcacatcg tttacaaaaa tgaagagttt
      241 gcggtgaaca tcgatcgaat cgtttcgccc gaaatgccgg gctggtatgt ggaaatcaaa
      301 agtcgtacat ggtcgccgcg cgacgcagaa gcgaaggccg ccttaatcgc ggaaatgctg
      361 cggctgttgg gcgtggagcc ggacaaggtc atgcgcacgg agtacgtcga gcagctataa
      421 ctgctcacca ctgggacgca gagacgccga gttattaact cacctgacgc acatcaagtc
      481 ggttgtcatt cccgcgccct tcggtggtaa aacccctcag ggtgtaccaa aggtgggaat
      541 ccagagcgga atgactggac aaccgctttc gcgggtgtga cagcccgcac ggtgaattaa
      601 tccttcagaa gtcccccact tttttgtgta aatacttggt gggggaacat tcaaagtcaa
      661 taacgaaaac gcggtcgatg gattttccat cgaccgcgtt tctcattcat taacaggctc
      721 gtatgattta gttagcggaa accacaaaac taccgatgtt cactatcgcg ccatcgattg
      781 atagtttcaa ttcatacgtg ccgggatcca gcggcgaact gccggtgctg ccgaattgaa
      841 tccagtcctc ctcggtcagg ccgaagttcc aagtgtgatc cttggagtcc gccggctcgc
      901 cgtttaagaa ccactgagtc tgccaggcag tatccttggg catgttaatg ccgctaaaga
      961 aggcatacac ttctttggtg ccggccggaa aagtcggatc gctaacctca acaggattta
     1021 ccggctgatc gctactatcc acaccgcccg caaaagtgat tgggccgaaa tcgggttcga
     1081 cattagcctc aacggtcact tggcctgact gcaccaagtt ttcgccgacg tacaacttaa
     1141 gttcccattg gccgctgcga actccgtctg gattgaagac ggtcagccac catacagaat
     1201 caggcccggc tgtggccgca tcccaattat ccgatgcact gagcttatct tgtaatttcc
     1261 cgttgaggta ccactcattc cgccacgcgt cgccattctt cacattcacg cctgagaaga
     1321 tggcatagat cattgttacg ccgccgggaa acgtgtccat tgcatcgaca ggctgttcat
     1381 gattctcgcc aatggcaaac gtgatgggcg agaatgatcc cccactgcca ttaggcaacg
     1441 gcgcttttgt gggggctggc gtttgagtgg ctgccggcgg tggaacaatc ggtgctttcg
     1501 tcgggacgat gttcggcaca gacgtgggca gcgcattgtt gatggccgca ttcactgtcg
     1561 ccgcgatttg ggtcgacagt gcattggtgt cgggcagatt gggttgtgcg ccgccacagg
     1621 cactgacgaa aagggctact actaagatca gaaaagatat gcgtttcata ttgagcctcc
     1681 taagcgcgca atgataggtg gattctcgat attcgtcaag ggtgaaattt tcgagaggtc
     1741 gaatccactg attgtgcatt ttttgacttt acagtagatc gattaatgtt gcgcggacga
     1801 tctgttcagc ccgatcaatc ctctgacccg acggcacttc gattaaaacc gcaatcgcat
     1861 aactcggcag atcggcaggg gccagcccaa tgaaccacgc caattggctg cttccgcgct
     1921 ataggcagtg ccgctgtgta aaactcgata acaaaacgcg gtcgatggaa ttttcatcga
     1981 ccgtaggctc agtcaggaac atgctcgcag gcgatcagtt agcgagaacc acgaaacttc
     2041 cgatgttcac taccgtgcca tcgatcgtga gtttcaactc ataggtgccg ggagtcagcg
     2101 gcgagctgct ggtgctgccg aattgaatcc agttttcctc gttcggactg agattccaag
     2161 cgtgattctt cgagtctagc gacttgccgt taaagaacca ctcgctttgc cagtctgtgt
     2221 cagcggtcac gttgatcccg ctgaagaacg cgtacacttc tttcgtacct gccgcgaacg
     2281 tcggtttacg aactgttacc ggatcaaccg gttgatcatt tgtgtccaca ccaccggcaa
     2341 acgtgatcgg accgaaatcg ggttcgacat tagcctcaac ggtcaccttt ccagactgca
     2401 ccaattgatc gccgatatac aacctcagtt cccattcgcc tgcgtccatg ccttcgggaa
     2461 acgacggcgt tatccaccac acgctgttga cgccggcgat tgcggctttc caggtcgttc
     2521 cagagctaag ttcggggaat gattcgccgt tcagcagcca ttcgtggcgc caggcttgac
     2581 catctttaat gttgatgccc gagaagaccc cgtagatctt ggtcacgcca ctagggaatg
     2641 tgtccatcgc atcgaccggc tgctcatttt ccacgccaat cgcgaacgtg atgggcgaga
     2701 atgaggcggt gctgttcggc ggtggattgg ctggcgcctg tgttgcggcg ggcggcacaa
     2761 taggtttggc ggtggccgct aattggggta cgctcgttgt gaccgaattg aatgcagcct
     2821 ttaccgtcgc cgcgatttgg gttgagagtg cattggtgtc gggcagattg ggttgtgcac
     2881 cgccacaagc actgacgaac atggctacta ctataatcag caaaccaatc tttttcatga
     2941 tgtccctccc aggcgcgcaa tgataagtgg aagatcgatt atcgtcaagt gtttcattcc
     3001 agtttatttc gaatcagcgg ttgccgctag cggctcttga tgagatcgat cagtgcagcg
     3061 cggccgatct gttcagcccg atcgatcctc tgacccgacg gcacttcgat caacaccgca
     3121 atcgcgtagc gcggccgatc ggctggggcc agcccgatga accacgccaa ttggctgctg
     3181 ccactaaagg caataccgct atgccccgcc gtattgtcga ttgtacgcat ggccgctaaa
     3241 acaatctctg ccgttgagtt ggaaatgatc cgttccgccg gaatagttgc gctgtcgctg
     3301 tgatatggct gccattcgcc atcgatcgat tgaaagtctt ggactagatg cacttgcggc
     3361 atgttaccat gattgccgat cgtcgcggcg accaatgcca tgtgcagcgg cgagatcgtt
     3421 aattgacttt gacccgtcgc aaaggcattg agcgcctcag ttgaagttaa ggccacagtg
     3481 gcggtcaagg cggctgctgt acgaatttct aatgaaggcg gtacatctaa atgccagtgc
     3541 tccgtgatcg agattaattc agtttcgcct aacgctattc taagatcggc gaacgatgcc
     3601 ggacaagcat tagcataagc ctcagccaat gttggagtgg cctctgatcg atagcaattc
     3661 aaaacgagac tgccgatcgc gatcgattga tcgggacgtg tcagcgtctg cgtgatcgaa
     3721 acgaaaccgc gatcgatcac atcggccaac agcggcgtct caaagatcgc gcccggctgg
     3781 aataaaccct gcgtagcgcg attgagcagc ggtgacgcag aatcgctcga cagcgttttc
     3841 caatttacat ccaacgagtt gggatcgaac gagggatagc ttgccagcgc gatgatcgcg
     3901 ccgtcgggta ttgacaccac gacagcgccg cccggccgat cgaattgatc cgttaacgcg
     3961 cgctgctcgg ccagatcgat agtcaagcgc acgctgcggc cggctcgctc gcgatgcagc
     4021 agctcatcga gaaacccgta cggcccgcgc agtgtttgat cgagcgcttc ttcaatctct
     4081 gatcggccaa agttgatcga gtaataaccc acggcgggca cagcagatcg atcgggataa
     4141 tgccggacga atgtgccact gactggaatt gtttcggcca agatccgatc gtatcgatcg
     4201 ataatcgcgc cgcgttgaat gcgctgctcg gcgatgatta gccgcgcgtt gtcgtcacgt
     4261 tcaaccaaac cgggcgatcc gatcgaccaa atgcctaaag ccagcgccag cacaccgaaa
     4321 gccgctaaga aagcaagtga gatgcgttga atggattttg tcatcgtgct gggcttaatc
     4381 tcgatcagcg gaaatttgcg acaacagccc gatcgccaga aaactgacca agagcgaact
     4441 cccgccgtaa ctgacgaagg gcaaggtcac accggttaaa ggtgcgacgt ttgcattgcc
     4501 cgccatgatg atccacgctt gtacgccgat cgatgttgcc aggccagctg ctaacaattg
     4561 tccgtaggtg gtgcgtgcac gcaaggcggc gcgtaagccg cgcagggtta ataaggaaaa
     4621 gcaaatgacc acagcaagag tcccgatcaa accaaattct tcgccgatag cggctaaagg
     4681 catatcagtg tgcgcggcag gtaaaatgat atccggcgat ccttgtccca gtccgctgcc
     4741 gatcgctccg cccgatccta acgcgatcaa cgattgcacg atttgatagg atgatccggc
     4801 cgagtccgtc cagggatcga tccaggtgct gacgcgacca cgcacgcggc cgatcagctg
     4861 aaacccgatc aaaccggcga cgatcaataa gagcacgccg actaatagat aacgcggctg
     4921 gctgctggcg agatacagca tgaccaagaa tgttaaatac aacaagatgg ctgtccctaa
     4981 atcctgctgt ccaatcaaca agatcaaagc gactgcccac atggcgatca gcggcgcgag
     5041 atagggcgcg gtcaaaaaac gctgatgttc aatttgaaga ctctgcgcaa tctcgcgctt
     5101 ctcggctaga tatgaagcca ggaaagcgac aaacagcagc ttaagcattt ccgaaggttg
     5161 aaagaaaact ccagcgagtc cgcccagcca caggcgcagg cccgatcctt ccggattgac
     5221 accgatcaca aaagtcagcg ccagcaaaaa caagccgccg attaaccatg tatatcgata
     5281 acgccgcagc cacttgaggt ttgccggtga ccatgtgatc gcaatcagtg cgctcgatcc
     5341 gacaatcagc cagatcactt gacggctgag gtaaccgggc gccaggcgcg ccacttcgat
     5401 caacccccag ccggtgagca gcgccgcgat cggcagcagg aacggatcgc gtccgcgtac
     5461 ccgtcgattg aaatgaagat ggatggctgt gaagcaaatg ctccaaacga gaaaagcaat
     5521 tagcactgac caattaacaa tgaagaatgt taaatgctcc tggttactga tcagctcgag
     5581 cagcacgaag ccgctcagcg tgaaggcacc ggccagaacc aacaagacac gctcacgcag
     5641 aatgttgatc ggcaaacgcg accataggta agccgtctca atcggctgag tgttatttaa
     5701 ttgcggattt tcgagtgcag attgcggagt acggagtgcg gattgcaaac tcaactgttc
     5761 atcctatcct cttgttactc ttctgcgtgg caacttcttc ttaacaactt tctttgcagc
     5821 cttagtcgca gcgatcggca tatacttgcc gatgatcggc gcgagatcgc gtttggtttt
     5881 gagcggcaca aattgaggct gagtgatgat cgcattcgct aaggccgttg ggcattcgca
     5941 cgtccgtcga ttcgggatgg ccggcgctag atatgcgatt gcccgtttgc tgacctcaac
     6001 gttcgccagc aagcgctcga tgatcatttg cacgttgact gtttcttcac ccgcatgcca
     6061 gacatcgtaa tctgtgacgt gcgccatgca cgcgtagcaa atctccgctt cacgcgccag
     6121 gttagcttcc gggatcgccg tcatgccgat gatgtccatg ccccattgtc ggaacaatgt
     6181 gctttcggct ttggtgctga agcgcggtcc ttcgatcgtg acgaaccagc cgcccatatg
     6241 cacggttgca ccagtgtttt taacggcctc gtataataag ccgctcaatt cgggacaaaa
     6301 cggatcggcg aggccgatgt gtgcgacaat tccattccca aaaaatgtca gcggccggcc
     6361 acttattcga tcgaataatt gcgagggaat gacgatgtgg cgcggctcaa attcttcacg
     6421 caaacttccg cacgcgctaa ccgaaacgat ccgttcgacg ccgatcgatt tcaacgccca
     6481 gatgttcgcg cgcgaattca cttccgtcgg tgtgaggcga tgcccacgcc catgcctcgg
     6541 caaaaatgcg acgcgctggc tggacaaact gccgaccacg atcgcgtcgc tgggatcgcc
     6601 gaatggcgtt ttgattttga attctttcac atcggtcagc gcttccatgt catataagcc
     6661 gctgccgccg atgatcgcga gttttacttt ttcaatattg agcatgagtt ccctctttcg
     6721 ttcgatgcgt gaaagtcatt gtatactggt gcaaatgatt ggtcaatcag ggactgctgc
     6781 gtgatgcata atgcgtgacg aatttttact tgagacaatt tatactgacg agtacctctt
     6841 ggccgtgaac aagccggcag gcttaccgac attggtcgat ggctacaaca aagatgctcc
     6901 atctctagtc ggattggtgc agcagcgata cggccgagtt tggatcgtgc atcggctcga
     6961 taaagacacc agcggagtgg tgctgtttgc ccgatcggct gatgtgcacc gatcgctcaa
     7021 cattgctttt gagactcgcc atgtctcaaa ggtttaccac gcgattgtga acggtgtacc
     7081 agaatggaat aaattggaga tcgaatttcc actgaaggcc gacggcgatc gacggcatcg
     7141 cacggtggtc gatcgcgctg aaggcaaagc ggctttaact cgctgccgcg ttttggaaaa
     7201 gtttaccgcc cacgcgctga ttgaagccaa acccgaaacc ggtcgcacgc atcaaattcg
     7261 cgcgcatctc gccgcgcagg gatttccgat cgttggtgat gaattgtacg gtcgtccggc
     7321 gcgggtgatc gatcgaacac tcatagatcg atcggctttg catgcgcgct cgatcacaat
     7381 cgcgtgataa attttttcga cgcggcgagt ctcaaacgcg atattgagtg ctcggtgcac
     7441 atcagccgat cgggcaaaca gcaccactcc gctggtgtct tta
//
LOCUS       DASRTJ010000487         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_258122,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000487 DASRTJ010000000
VERSION     DASRTJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258122"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFF70_09925"
     CDS             <1..744
                     /locus_tag="VFF70_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45053.1"
                     /translation="AFMRDVVQSALTANDSPIVAIDEHERWLREEARSGTSISFRWAF
                     NSETKTLADRVEAVVIDLLRSHAEWHPDELQREVYRRFPDQFTPDRALVATCIASYAD
                     EIEPGRVQLRSEDAFESREGEMKEAKESLEAIGQKLNYEVKSKPSAVIWLQDKVEVYS
                     FVISTLAEIDPMLRAESGVLVLPGGRASLLQFKLNRDPRLHESQWQTLKFRSLRHLTE
                     HTDLTLDVFALALGLEPPIEQAGTQIKLL"
     gene            complement(829..2286)
                     /locus_tag="VFF70_09930"
     CDS             complement(829..2286)
                     /locus_tag="VFF70_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HZY45054.1"
                     /translation="MTFDTLRPVDRSRIIVPMNPQHYQRSVKRVLIGIMLINLIVTAI
                     KFFVGALTGSIAVLADAFNALIDAASNVIGLFGIYAASDPPDPDHPYGHRKYETVATL
                     GIAALMLVAGWEILQNVFRRLSSGSQPVVTTEALLLILITLPLNTLVVWYEGRRGREL
                     RSDILLADATSTRINIFVSLSALIGLLGSSIGLSWLDVIMALGIVGYVARAALKIVRE
                     TSDVLTDSAVIDPHVVEGVALSVPGVWFATKVRSRGRGDDVHLDLHIKVDPAMATEQA
                     HALSDEVEHRLQEKIPGLVDAVVQIEPGEHAPLSDWDAIAVRLRAIADGLGLGVHNLH
                     IDVIPTGYLVDVDVEVNSDFSLGQSHALVTQLEARARAELPQVTEIVTHIEPMEPGDT
                     TRPIVGRDAEALRASIIRIGDRVAGIGTCHHVILRRSPSLDRVDMSLHCVLPGESPVV
                     AAHLIAEQIERQLRAELTQLDHITIHVEPPEARDQ"
     gene            2423..>2625
                     /locus_tag="VFF70_09935"
     CDS             2423..>2625
                     /locus_tag="VFF70_09935"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45055.1"
                     /translation="MQHSEGKFTTADGIHIYTQQWVADQPIANIVIVHGLGDHSDRYK
                     NYVDYFVPRGYSIYSFELRGHGRS"
BASE COUNT          610 a          722 c          730 g          563 t
ORIGIN      
        1 gcgtttatgc gcgacgtcgt ccaatcggcg ctgacggcga atgactcgcc gatcgtcgcc
       61 atcgacgaac atgagcgctg gttgagagaa gaagcgcgat ccggaacttc gatttcgttt
      121 cgttgggcat tcaattccga aacaaaaacg ttggccgatc gagtggaagc ggttgtgatc
      181 gatttgctcc gatcgcatgc cgagtggcac cctgatgagt tacagcgcga ggtttatcgc
      241 cgcttccccg atcagttcac acccgatcgg gcgttggtgg ctacttgcat cgcatcgtat
      301 gcagatgaga tcgagccggg tcgagtacaa ttaagaagcg aagacgcatt tgaatctcgc
      361 gaaggtgaaa tgaaagaagc caaagaaagt ttagaagcaa tcgggcagaa gttgaattac
      421 gaagtcaaga gcaaaccgtc ggccgtgatt tggttacaag acaaagtgga agtttactcg
      481 ttcgtgatca gcaccttagc cgagatcgat ccgatgttac gcgctgaatc cggtgtgttg
      541 gtgctgcccg gtggccgcgc ctcccttttg cagttcaagc tgaatcgcga tccgcgcctg
      601 catgaatcac agtggcagac gttgaagttc aggtcactac ggcatttaac cgagcacacc
      661 gatctcacgt tggatgtttt cgccctcgcg ttagggcttg aaccaccgat cgagcaggcc
      721 gggacccaga ttaaacttct ttagtggatt acccttcgtc aatgccaagg tgactccctg
      781 aataggtgac aaagtgattg ggttgatagt cgcacgctgc ttagcctgtt actgatctct
      841 cgcttccggt ggttcaacat gaatcgtaat gtgatcgagc tgagtcagtt cagcgcggag
      901 ctgacgttcg atctgttcgg caatcaagtg cgccgcaacc accggggatt cgcccggcag
      961 gacgcagtgc agcgacatat ccacacgatc gagtgacgga ctgcgccgta agataacgtg
     1021 atggcaagtg ccgatgcccg ccacccgatc gccgatgcga atgatcgatg cgcgcagcgc
     1081 ctcggcatca cgcccgacga ttggccgagt cgtatcgccc ggctccatcg gctcaatatg
     1141 cgtcacgatc tcagtgacct gtggcagttc ggcgcgcgcc cgcgcttcca actgcgtgac
     1201 taacgcatgc gattgtccca gcgaaaaatc cgaattgact tccacatcca catcgaccag
     1261 ataaccggtt ggaatcacat cgatatgcaa gttatgcaca cccagcccca atccatctgc
     1321 aattgcgcgc aggcgcacgg caatggcgtc ccagtctgat aacggcgcat gttcacccgg
     1381 ttcaatctgc acgacggcat ccaccagccc cggaattttt tcctgcaggc gatgttcgac
     1441 ttcatccgaa agcgcgtgag cttgttcggt cgccatagcc ggatcgactt tgatgtgcag
     1501 atcgagatgc acgtcgtcgc cacggccccg gctgcggact ttggtggcga accacacgcc
     1561 cggcacactc agcgcgacac cttcgacgac atgcggatcg atcacggcgc tgtccgttaa
     1621 cacatccgat gtttcgcgca cgatcttcag tgcggcgcgc gcgacgtatc cgacgatgcc
     1681 gagcgccatg atcacgtcga gccagctcaa tccgatcgag ctgccgagca aaccgatcaa
     1741 ggccgacagt gacacaaaga tgttaatgcg cgtgctggtc gcatcggcca ataaaatatc
     1801 gcttcttaat tcgcggccgc gtcggccttc ataccaaacg accaaggtat tcagcggcag
     1861 tgtgatcaaa atcagcagca aggcttcagt cgtcacgacc ggctgactgc cgctgcttaa
     1921 acgcctaaaa acgttttgca ggatttccca accagccacc agcatcagcg cggcgatgcc
     1981 cagcgtggcg accgtttcat atttgcggtg gccgtaggga tgatcggggt cgggcggatc
     2041 ggacgcggca taaatgccga acagaccgat gacgttggag gccgcatcga tcagcgcatt
     2101 gaacgcgtcg gccagcacgg cgatcgagcc ggtcagtgcg ccgacgaaaa atttgatcgc
     2161 ggtcacgatc aagttgatga gcatgatccc gatcagcacg cgcttaacag agcgttgata
     2221 gtgttgagga ttcatcggga cgattatacg tgatcgatcg acgggtcgca acgtgtcaaa
     2281 tgtcattgtt gatcaaatta acttacccag ctggagccgt gtcaggcggt ggtgggtgaa
     2341 ggctagtatc agcgtcgcat tgactttaag tctatatcaa gggtacgatg tgaaggaaac
     2401 aggccggcag gagttcaaat tcatgcagca ttccgaaggc aaattcacta ccgccgatgg
     2461 catccacatt tatacgcaac aatgggtagc cgatcagccg atcgctaaca tcgtgattgt
     2521 ccacggcctg ggcgaccaca gtgatcgcta caaaaactac gttgactatt tcgtgccgcg
     2581 tggttattcg atctatagtt tcgagctgcg cgggcacggt cgatc
//
LOCUS       DASRTJ010000488         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_105921,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000488 DASRTJ010000000
VERSION     DASRTJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105921"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..932)
                     /locus_tag="VFF70_09940"
     CDS             complement(207..932)
                     /locus_tag="VFF70_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HZY45056.1"
                     /translation="MSSLNMPDTPQKESAFWKISFVVLMGVIGFLVVTLIAMLSNTTP
                     FLMIDQTIDKLFKIDTVQSMWYITRAAGLIAYVLLWLSTVWGLAVSSKIIDPLVQRGS
                     SYDFHQVLSLLSIAFIVLHIVVLMFDQYMPYTLAQILIPFTSPYRSVWVGIGVIALYL
                     TVLVSVTFYIRRWIGQKAFRTIHYLSLIAFIGSAIHGLFSGTDSPLWTVQAMYSFTSL
                     SVVFLTVYRIAASRPKRQRSLSH"
     gene            complement(910..>1803)
                     /locus_tag="VFF70_09945"
     CDS             complement(910..>1803)
                     /locus_tag="VFF70_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZY45057.1"
                     /translation="AEGEANALAHGFAQARSFIEAGEARFTRFSEASELAQLNRSSGT
                     WFLASPEMFDVVTQARAYAEETEGIFDPMILDVLEGAGYDKSMDDIRAHGVSPSTRSA
                     IPARSDFREIQLDPAGRSIRLPIGSRIDLGGIAKGWIAEQAALRLSEYSEACVVNAGG
                     DLFAIGQPSDQPSWSIGLEDPRAAAQTLAVLGVQSGAVATSSIMKRRWQQGDRIQHHL
                     IDPRVGRPANTDWLSVTVAAPHAALAEVYAKVLLIVGSSDAVRLSERRPEIGFIAIDA
                     AGQLWGSVQSREMLNVQFEYA"
BASE COUNT          463 a          531 c          449 g          360 t
ORIGIN      
        1 ctagtaactc accttacgca ttgtcgcacc cgcaaaagcg ggtgtccaat tatttcattc
       61 tggattcacc caccactccg ctcacctgca ctgctccgtg gtacatcctg cgtctgcacc
      121 atcgcgggac attgcggtgc aagtgtcgct gcgagtgtct tcgactttcg cgagaatgac
      181 agtaaggtca atttgcattc agtgatctaa tgacttagac tacgctgcct ttttggccgt
      241 gacgccgcaa ttcgataaac cgtcagaaag acaacactta agctcgtgaa actatacatc
      301 gcctgcaccg tccacaacgg cgaatctgtg cctgaaaaca gaccatggat cgccgagccg
      361 atgaaggcga tcaaactcaa gtagtgaatc gtgcggaacg ctttctgacc aatccagcgc
      421 cggatataga acgtgacgga caccagcaca gtcagataca gtgcaatgac gccgatgccc
      481 acccaaacgg atcgataagg ggacgtgaac gggatcagga tttgagccaa agtatacggc
      541 atgtattgat cgaacatcaa cacgacgatg tgcaagacaa tgaacgcaat cgacaacagc
      601 gacaagacct gatgaaagtc gtaagaggaa ccgcgctgca ccagtggatc gatgatcttg
      661 ctggagactg ctagacccca gacagtcgac agccataaca gcacgtaagc gatcagccct
      721 gcggcgcgag tgatatacca catcgattgc accgtgtcga tcttgaagag cttatcaatc
      781 gtctgatcga tcatcaagaa cggcgtggtg ttcgacagca tcgcaatcag tgtgacgacc
      841 agaaagccga ttacacccat cagcaccacg aatgaaattt tccaaaaagc agattctttt
      901 tgaggcgtat caggcatatt caaactggac atttaacatc tccctcgatt gaactgaacc
      961 ccataattgt ccagcggcgt cgatcgcaat gaaaccaatt tcgggccgcc gctcactgag
     1021 acggaccgca tcgcttgagc caacaatcaa cagcactttc gcatacactt cggccagcgc
     1081 ggcatgcggc gccgccaccg tcacactcaa ccaatcggta ttcgccggcc ggcccacacg
     1141 cggatcgatc aagtgatgct gaattcgatc gccttgctgc caacgccgct tcatgatcga
     1201 tgaagtagcc acggcgcccg attgcacacc caacaccgct aaagtttgag ccgcagcgcg
     1261 tggatcttcc aagccgatcg accaactcgg ctgatcggac ggctgaccga tcgcgaacaa
     1321 atcaccgccc gcattcacca cgcacgcttc ggaatattca gacaaacgca atgcggcttg
     1381 ttctgcgatc cagcctttgg cgatgccgcc cagatcgatc cgcgaaccga tcggcagacg
     1441 aatcgatcga ccggccggat caagttgaat ctcacgaaag tccgatcgag ccgggatcgc
     1501 cgatcgggtt gaaggcgaaa cgccgtgtgc ccgaatgtca tccatgctct tatcgtaacc
     1561 ggcaccctcc aacacatcga gaatcatcgg atcgaaaatg ccttcagtct cttcggcata
     1621 cgcccgcgct tgtgtgacga catcgaacat ttcaggcgag gccaggaacc aggtgccgct
     1681 cgatcgattg agttgggcca gttcgcttgc ttcggaaaag cgtgtgaagc gggcctcgcc
     1741 ggcttcgata aacgatcggg cttgcgcgaa gccgtgagcc agtgcgttcg cttcgccctc
     1801 ggc
//
LOCUS       DASRTJ010000489         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_68852,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000489 DASRTJ010000000
VERSION     DASRTJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68852"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1285)
                     /locus_tag="VFF70_09950"
     CDS             complement(38..1285)
                     /locus_tag="VFF70_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45058.1"
                     /translation="MDQLHKRYTAEQVKVLLHGYGQGTLSRAEVQEMLQINKTRFFAL
                     LKDYRASPEKFNLSYERHSPAKLTARAENEIKKALLQEKELVNDPELPIADYNYTALR
                     DRLQQKGLRVSTTTITKRAKQLDCYKPHRKGKVHDREVVTASTGALVQHDASLHLWSP
                     YAAEKWTLITSLDDYSRLLLFADFFAHESSWAHIQAAESLMQHYGLPLRYYVDNLRVF
                     RFVQGRDSYWRNHVLTTDAVDPQWRQVMRLLGVDVTHALSPQAKGKIERPYRWLQDRI
                     VRTCALEQITTLDDARSVLKHELDRYNNHQVHSTTGEIPSLRFAQACEANSLFRPLAL
                     PQPYTSAKDIFCLRVTRRLDGYRRISLFKHELVVPKVPVREDVEIHLIPNAAKQVMDV
                     RVWWDRKLVQTVSLPLAGFPVHF"
     gene            1805..2842
                     /locus_tag="VFF70_09955"
     CDS             1805..2842
                     /locus_tag="VFF70_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45059.1"
                     /translation="MFTQQLTIDSIEALLPEGYTARGATMDDLEATVAVFNACARALN
                     SGEEFNAIDFRSEWEVPNLDLSHDLRLVISPAGQIVGYMEFWDLSEPHIRYRAWGRVH
                     PDHLNRGLGTYLVRWAEQRAQQAIPRALQESRIWMTSWVNDLNLRAKLLFENCGWHVI
                     RKNYRMAIELTAPPADPIWPDGITLRTFVPGQDERATVQADRDAFRDHWGYVERPLEA
                     EIKMLKHHMSKPGFDPTLWLLGMDGHRVAGKCLNDPQAEDDPDMGWITDLGVRREYRR
                     RGLGQALLQYSFADFYRRGKRKVGLNVDASSLTGALRLYEHAGMHVVRVNSTYEKELR
                     AGVDLSTQSIS"
     gene            2863..>3257
                     /locus_tag="VFF70_09960"
     CDS             2863..>3257
                     /locus_tag="VFF70_09960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45060.1"
                     /translation="MLTQQLTLDSVESLLPAGYTSRGATLADLVPAVAMFNAYSQGLY
                     GKDEITAKEYRLEWEAPNLDLANDVRLVLAPDGQIVGCMEFWDLTEPHVRYNTWGRVH
                     PDHADRHIGTYLVCWAEERAQQSLPHAAEA"
BASE COUNT          711 a          823 c          939 g          784 t
ORIGIN      
        1 atgttagcca aacttgaaaa gtgaactcag tcaaacttca aaagtgaact gggaaacctg
       61 ccaatggcag actgacggtc tgaaccaatt tgcggtccca ccagacgcga acgtccatca
      121 cttgtttagc cgcattcgga atcaaatgga tctcgacgtc ttcgcggacg ggcaccttcg
      181 gcactaccag ttcatgtttg aagagggaaa tgcggcgata accatctagc cgtcgggtga
      241 cgcgcaggca gaaaatatct ttggcagaag tgtagggctg cggtagagcc aacggccgga
      301 ataaactgtt ggcctcgcac gcctgggcga agcgcagact ggggatctcg ccggtggtgg
      361 aatgcacttg atgattgttg tagcggtcga gttcgtgttt caagacggag cgcgcatcgt
      421 ccagcgtggt gatctgttcc aaggcgcagg tgcgcacaat gcggtcttgc aaccagcgat
      481 agggtcgttc gattttgcct ttggcttggg gcgagagcgc atgcgtgacg tccacgccga
      541 gcaggcgcat cacctggcgc cactgcggat cgaccgcgtc ggtggttagg acgtggttgc
      601 gccaataact gtcgcggcct tgcacaaacc ggaacacccg caagttatcg acgtagtacc
      661 ggagcggcaa gccgtagtgc tgcatgaggc tttcggcagc ctgaatatgg gcccaactgg
      721 attcatgcgc aaagaaatcg gcgaagagca acaggcggct gtagtcgtcg agcgaagtga
      781 tcagcgtcca tttctcggcg gcatacggtg accagagatg tagcgaagca tcatgttgca
      841 ctaaggcgcc ggtcgaagcc gtcaccactt cgcgatcatg gaccttgcct ttgcggtgcg
      901 gtttgtagca gtccagttgc ttggcccgtt tcgtgatcgt cgtcgtcgac acgcggaggc
      961 ctttctgctg gaggcgatcc cgcagggccg tgtaattgta atcagcaatg ggcaactcgg
     1021 gatcgttcac cagttctttt tcctgcagca aggctttctt gatttcgttt tcggcgcgcg
     1081 cagttaactt ggcgggcgag tggcgttcat aactgaggtt gaacttttcg ggactggccc
     1141 ggtagtcttt cagcaacgcg aaaaagcggg tcttgttgat ctggagcatc tcttggacct
     1201 ccgcccggct cagggtgcct tggccatatc cgtgcagcaa aaccttgact tgctcggccg
     1261 tatagcgctt gtgcaattga tccatggtgc tccttgtttt agtgagcagt ctatcaggat
     1321 cgttcggcct ttggcacttt tgcgcagttc acttttcaag tggtaacggt tcgcttttca
     1381 agttggaacg gttcactttt caagtttgga taacagaata catttgcatc ttgacaaatg
     1441 acctcccact atggtaaact gcccgcgttg taaattttag aaaggaggcg cacccaaatg
     1501 gataacatta atttcttgac tggcaaattt gtggcccgaa ccgtcagcgg tttggcttct
     1561 ttgccttatc ggagtgcgcc tgttactcgg tagacagaac ttttgctgtc gaaggccgtg
     1621 gggctcactc tccacggact ttttgtttgt atagtgcgag atgcactgaa gacagaggcc
     1681 gtggagtcta actctcggcc tctttttacg agcaaatact gcggactgcg tgttgcgtaa
     1741 ttcgcgtgtt atgcagcacg cactactcag gtcgaatcat gtctcacaca ttcaggagtc
     1801 aaatatgttt actcaacaac ttaccatcga ttcaatcgaa gcactactac cagaaggcta
     1861 caccgcgcgc ggcgcaacga tggatgatct cgaagcgacg gtggcggtgt tcaatgcttg
     1921 tgcgcgtgcg ctgaacagcg gggaggaatt caacgccatc gattttcgat cggaatggga
     1981 agtacccaat ctggatttgt cgcatgacct gcgcttggtt atctcgcccg ctggacaaat
     2041 cgtcggttat atggagttct gggatttgtc cgagccgcac attcgatatc gcgcgtgggg
     2101 ccgtgtacat cccgatcatc tcaatcgcgg tctgggtacc tacctggtgc gttgggccga
     2161 acagcgtgcg cagcaagcga tcccgcgtgc actacaagaa tcgcgtatct ggatgacaag
     2221 ctgggtcaac gatctcaatt tgcgcgcgaa gctgctgttt gaaaattgtg gctggcatgt
     2281 gatccgcaaa aattatcgca tggcgattga actaactgcg ccgcctgctg atccgatttg
     2341 gccggacggt attacgctgc gaacctttgt gcccggccag gatgaacgcg ccacggtcca
     2401 agccgatcgc gatgcattcc gtgatcattg gggctatgtg gagcggccgc tcgaagcaga
     2461 aatcaagatg ctcaagcatc acatgtccaa gcctggcttc gatccgacgc tgtggttgtt
     2521 gggcatggat ggccatcgag tcgctggcaa atgcttgaat gatcctcagg ctgaagatga
     2581 tcccgacatg ggctggatca ccgatctcgg tgtacggcgc gaatatcgac gccgcggatt
     2641 gggccaggcg ctgcttcaat attcattcgc ggatttctat cggcgcggca agcgcaaggt
     2701 gggtttgaat gttgatgcca gcagtttgac tggcgcgctg cgtttgtacg aacacgctgg
     2761 tatgcacgtg gtgcgcgtca acagcacgta tgaaaaagaa ttgcgagcgg gtgtagatct
     2821 cagcacccaa tcgattagtt gaagcacaaa aaggaaacga ccatgttaac tcaacaactt
     2881 actctcgatt cagtcgaatc actgttgccg gcaggctaca cgtcacgtgg cgcaacgctg
     2941 gctgatcttg tgcctgccgt ggcgatgttc aacgcttact cgcaggggtt gtatggcaaa
     3001 gatgaaatca ctgcgaaaga atatcgcctg gaatgggaag cacccaatct cgatctagcg
     3061 aatgatgtgc gattagttct tgcacccgat gggcagattg tcggctgcat ggagttctgg
     3121 gatttgactg agccgcacgt tcgatacaac acgtggggcc gcgtacatcc tgatcatgcc
     3181 gatcgccaca tcggcactta tctggtgtgt tgggccgaag agcgcgctca acaatcgctg
     3241 ccgcatgcgg ctgaagc
//
LOCUS       DASRTJ010000490         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_278365,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000490 DASRTJ010000000
VERSION     DASRTJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278365"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFF70_09965"
     CDS             complement(<1..583)
                     /locus_tag="VFF70_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HZY45061.1"
                     /translation="MIDLRQFEVWFVTGSQHLYGNEALEQVAEHAKVIVQALGRSRHI
                     PVNLVFKPVVTTPEAIYELCLEANAAKNCIGLITWMHTFSPAKMWIAGLVTLKKPFLH
                     LHTQFNQDIPWSTIDMDFMNLNQSAHGDREFGYIGSRMRLNRKVVVGHWQDEDVQTRI
                     GVWTRAACAWHDAQGLRIARLGDNMRQVAVTEGD"
     gene            complement(714..1739)
                     /locus_tag="VFF70_09970"
     CDS             complement(714..1739)
                     /locus_tag="VFF70_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZY45062.1"
                     /translation="MHVTLQDVARQAGVSTKTVSRVVNNQGEISETTRQRVQAAIEQL
                     GYYPNILARSLVNQRSNTLAVVAWGIDYYGPSRILVGIEKKSDELGYSLFLNLLARPD
                     DSHIDRILDMLIARRVEGILWAVPEVGNNRAWIATARLDSLPPVVFVSMASRPGLTTV
                     SIDNRHGAAQATQHLIDQSPRHIGMITGPLTWWEARERQAGWQAALQKAGLTPAPALV
                     VEGDWSAASGDRAMRLLLDREPAIDAVFVCSDQMALGALGAAHQAGRKVPQDLIIAGF
                     DNIPESAFFWPPLTTVYQQLVDAGGIAVQNLHQVIEGRRKDNQHPQSEATILTPELVI
                     RESSVQR"
     gene            2424..2507
                     /locus_tag="VFF70_09975"
     CDS             2424..2507
                     /locus_tag="VFF70_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45063.1"
                     /translation="MVTRYQKRISGPLLDRFDIHIEVPRVN"
     gene            2596..2808
                     /locus_tag="VFF70_09980"
     CDS             2596..2808
                     /locus_tag="VFF70_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45064.1"
                     /translation="MTAAHIREYCAVDDAGKSLTRTAMQQLGMSARSFHRVLKLARTI
                     ADLADSDQIQSAHLAEALQYRPRQQS"
     gene            complement(2905..>3723)
                     /locus_tag="VFF70_09985"
     CDS             complement(2905..>3723)
                     /locus_tag="VFF70_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45065.1"
                     /translation="VQSCATAGRPVAVVKCVGDFGAAFDAKAASPLTLTVGRVEEAGP
                     SHTDMRDWNPANYSSAAAAATLYYQLVKPIWNLNPLIDVWETFNEKSANWNWQADFYI
                     SLMDLAEADGYRLGLWSASVGNPPEWAYPQIARACQRARQSGNHILCLHEYGGIWNTD
                     DGRLKTESPLLVTRYRQLYSYLEQQAAVIPLVISECGENGGGGFTGTDVFIDDFSWYD
                     DQMMRDSFVIGCAAWTLGGWDGANFRTALPALTTYIINKVKVNRIYLPLITNSS"
BASE COUNT          868 a         1004 c          982 g          869 t
ORIGIN      
        1 tatcaccttc ggtcaccgcg acctgacgca tgttgtcgcc caatcgagcg atgcggagac
       61 cttgagcatc atgccaggcg caggcggcgc gcgtccacac gccgattcta gtttgaacat
      121 cttcgtcctg ccaatgaccg acgacgactt tgcggttcaa gcgcatccgg ctgccgatat
      181 aaccaaattc gcgatcgccg tgcgccgatt ggttgaggtt catgaagtcc atgtcgatcg
      241 tcgaccaggg aatgtcttgg ttgaattggg tatggagatg caggaatggt tttttcaacg
      301 tgaccaatcc ggcgatccac attttggccg gtgagaaggt atgcatccag gtgatcagac
      361 caatgcagtt cttggcggca ttcgcctcca gacacagctc gtagatcgct tccggcgtcg
      421 tcacaaccgg tttgaaaacc agattgacgg ggatgtggcg agatcggcca agcgcttgca
      481 cgattacttt tgcatgctca gcgacttgtt caagtgcctc attgccatac agatgttggc
      541 tgcctgtcac aaaccatact tcgaattgtc gtaagtcaat cacggtagat gctcctgtgt
      601 cacctgctag aaaaatatcg atctgaaacg ataagcaggc ggatagaatg gatcggttag
      661 gccgctgggg ttttgcctca agacaacgcg agcggcgcga atattcacta gcctcaacgc
      721 tggaccgaac tctcgcgaat cacaagttca ggcgtcaaga tcgtagcttc ggactggggg
      781 tgttggttgt cctttcggcg tccttcgatc acctgatgca aattttgcac ggcgatgccg
      841 cccgcatcaa ccaattgctg atagacggtc gtcagcggcg gccagaaaaa tgcggactcg
      901 ggaatattgt cgaatccggc gatgatgaga tcttggggaa ctttacggcc tgcttgatga
      961 gccgcgccta acgcgccgag cgccatctga tcgctgcaga caaataccgc atcgatcgcc
     1021 ggttctcgat ccagcagcaa ccgcatcgct cgatcgccgc tggctgccga ccaatcgcct
     1081 tcaaccacta atgcaggagc aggtgtcaat ccagcctttt ggagggccgc ctgccagccg
     1141 gcttgccgtt cgcgcgcctc ccaccaagtc agtggaccgg taatcattcc aatatgccgg
     1201 gggctttgat cgatcaaatg ctgcgtcgcc tgcgctgcgc catgccgatt gtcgatcgag
     1261 accgtcgtga gcccggggcg tgatgccatg cttacaaaga caacgggggg taagctgtcc
     1321 aagcgggctg tagcgatcca cgctcgattg ttgcccacct ccggcacagc ccagaggatg
     1381 ccttcgacac gacgagcaat gagcatatcc agtatgcgat cgatgtggct gtcatcgggg
     1441 cgggcgagta gattgaggaa caacgaatag cccaactcgt ccgacttttt ttcgatgcca
     1501 accagaatgc gtgaaggacc gtagtagtca attccccagg cgactacggc caacgtgttg
     1561 cttcgctgat tgactaagct gcgcgccagg atgttgggat aataaccgag ttgttcaata
     1621 gcagcttgga cacgctggcg ggttgtttca ctgatctcgc cctgattgtt cacgacacga
     1681 gatacggtct tggtggagac acccgcttgc ctggctacat cttggagggt gacatgcata
     1741 tgaccgcctt ttttagcgaa gcgagcagcg cagcaatgtc caatgctatt attacgagga
     1801 gcagttgtat ttcaatgaag gcgtgtcttt gagactactt cgatgacgac agcgataacg
     1861 acaacgctgt cgttagtgta tgatggtttt cttggcttgt caatgggtaa tttttgaaaa
     1921 atttgtaaca taagataata acttgaaatc gatactgtac accagatcaa ccgagagttg
     1981 ccagttgtga attgtctggg gcgattggct tgattagtta actgctgagg ttgaggcaaa
     2041 ccaccgcggg cttgagcggt ttcaatcaag cactgtgcaa gacattagct agaatcagaa
     2101 cgctgtgctt atactcgctg atgaggatgt gctcgtgggg agtatgatcg agatcggaat
     2161 cgcccggccc gtaagccagc gcctcagccg cctgctgaaa gaaactctga gcgaggcacg
     2221 ctattcttct gcttcattgc cttgcagcta tcacgattca tgaaataatc gagaaatcga
     2281 tctaccgaac gagacaactc gtaattctga ctttcctgca attgtggctc tcaattgata
     2341 gtgaatcaag cttgttttgt tctcctccct gcccatgcgg ctatcacggc gaccccactc
     2401 aatcctgcac ctgttcaccc tcgatggtca cgcgttatca aaaaagaatc tccggcccgc
     2461 tgctcgatcg attcgacatt cacatcgaag tgccgcgcgt gaactaggag aagctgaccg
     2521 ccgatcgatt catcggcact tcattaatct gcaacgccga tacacctgca ctttcaccgt
     2581 agcgcaggtg caagtttgac cgcggcgcac atccgcgaat attgcgccgt ggacgatgcg
     2641 ggcaagtcgc tcacgcgcac ggcgatgcag cagctgggca tgagcgcgcg atcattccat
     2701 cgcgtgttga agttggcgcg cacgattgcg gatttagcgg attcggatca aatccaatca
     2761 gcgcatttgg cggaggcgtt gcaatatcga ccgagacagc aaagctgatc gatcgggatt
     2821 gtattgcatc gcagaaaaaa taacaacgcc cgatcaatcg aaatcgatcg ggcgttgttc
     2881 tcagttgagc agaacgcatt cagatcaact agaatttgtg ataagtggca agtatatgcg
     2941 gttaacttta actttattga taatgtaggt ggttagagcc ggcagtgctg ttcggaagtt
     3001 tgcaccatcc caaccaccca gcgtccaagc cgcgcagccg atgacaaagc tgtcacgcat
     3061 catctgatcg tcataccaag aaaaatcgtc aatgaataca tccgtgccgg tgaatccgcc
     3121 gccgccgttt tctccgcact ctgaaatgac cagcggtatc acggcggctt gttgctcaag
     3181 atacgaatac agttggcgat atctcgtcac cagcagaggt gactctgttt tcaaccgtcc
     3241 atcgtccgta ttccaaatac ccccgtattc atgcaggcac aggatgtggt ttccgctctg
     3301 tctagcccgt tgacaagctc gggcaatttg aggataggcc cactccggcg ggttgccgac
     3361 actcgccgac cataatccca accgataccc atcggcttca gctaagtcca tcaggctgat
     3421 atagaaatcg gcctgccagt tccaattcgc cgacttctca ttgaaggttt cccagacatc
     3481 aataagcgga ttgagattcc agatcggctt caccagctga tagtatagtg tggcggcggc
     3541 cgcggcgctg gagtaattag cgggattcca gtcccgcata tcagtatgcg atggtcccgc
     3601 ttcctcgaca cgaccgacgg tcaacgtcaa cggactggca gctttcgcat caaaagctgc
     3661 accgaagtcc cccacgcatt tgacgaccgc cacaggacgt cccgccgttg cacagctttg
     3721 gac
//
LOCUS       DASRTJ010000491         1821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_69047,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000491 DASRTJ010000000
VERSION     DASRTJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69047"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFF70_09990"
     CDS             complement(<1..241)
                     /locus_tag="VFF70_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45066.1"
                     /translation="MINQPWLINSLASMLTVLGVYLLGRDLFDQNTGLLAAALGSISP
                     MFVMLSGTLLAHPSAMAALIFFAWMFVRARRSDEPK"
     gene            complement(344..523)
                     /locus_tag="VFF70_09995"
     CDS             complement(344..523)
                     /locus_tag="VFF70_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45067.1"
                     /translation="MNLLKRMNRKVLVLCIATFVLATAVAWGVFDAMPHLEDEQANLF
                     QAKVFASGYVTVSEP"
     gene            complement(695..1177)
                     /locus_tag="VFF70_10000"
     CDS             complement(695..1177)
                     /locus_tag="VFF70_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45068.1"
                     /translation="MYFVTAIDGFGVATAGVQVAGGGNAWSALSDRNITRVIRADEAL
                     AKTPIVMLTARVESGDKIMGLDIGADDYITKPFNPNEVVSRVRAVLHRSTGDPVQAHV
                     IQLGGLLCDIDRYEVPVAASEEEIQAKALATEGAHRFMEGKPAKKIVYVAGKLVSIVV
                     "
     gene            complement(1442..>1821)
                     /locus_tag="VFF70_10005"
     CDS             complement(1442..>1821)
                     /locus_tag="VFF70_10005"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY45069.1"
                     /translation="PETHTYLKNTVIADSTSGGNCKGKAFDSSKYSLSSDNTCALSGT
                     GDQNSVAAKLNPLGLVGGSLKTHLPLPDSPLIDLISGSDYPSTDQRGIGRPQGLGGDT
                     GAVERQISDPVYSLLSYLPLVLK"
BASE COUNT          410 a          535 c          478 g          398 t
ORIGIN      
        1 gtttaggttc gtcgctgcgt cgggcgcgca caaacatcca tgcgaaaaag atcagcgccg
       61 ccatcgcgct gggatgtgcg agcaacgtgc cactcaacat cacaaacatc ggcgagatcg
      121 atccgagtgc ggcggcgagc aagcccgtgt tttgatcgaa cagatcgcgt cctaacaagt
      181 acacgcccaa aaccgtcagc atcgacgcaa gggaattgat cagccacggc tgattgatca
      241 acacgccgat cgcgagcagc agcggatagc cgggcgtata tttgccaaag agttgtccgt
      301 ctttgttgac gatgaacgga atcgagaaag cctgcggcgc gatctagggt tcagacactg
      361 tcacatagcc gctggcaaac actttggcct gaaacaaatt cgcctgctcg tcctccagat
      421 gcggcatcgc gtcgaacacg ccccatgcga cagcggtcgc taaaacaaaa gtggcgatgc
      481 acagaacaag caccttgcga ttcatgcgct tcaataaatt catcacgaag attcgatacc
      541 ccgttggttg ttcacgcgaa gcgatattat aaacgaatct gctgttgatg ttcgtgacta
      601 tgaattgcac aggccgctga gaatcgatcc tcagcatcct gtgttaaatg ctgacaacga
      661 gcgacatacc ttcaaagtgc atcgctcgtt gttgctaaac aacaatgctc actagcttcc
      721 ccgcgacata cacgatcttc ttcgcaggct tgccttccat gaatcgatgt gcgccttcgg
      781 tcgccagcgc cttcgcttga atttcttctt cactcgccgc caccggcact tcataccgat
      841 cgatatcgca caacaggccg ccgagttgaa tgacgtgcgc ttgaacggga tcgccggtcg
      901 atcggtgcag cacggcgcgc acgcgcgaga cgacttcgtt gggattgaag ggcttggtga
      961 tgtagtcgtc cgcgccgatg tccagcccca taattttatc gccgctctcg acgcgcgcgg
     1021 tcagcatgac gatcggcgtc ttcgccagcg cttcatcggc gcgaatcacg cgcgtgatgt
     1081 tccgatcgct cagcgcactc caggcattgc cacctcccgc cacttgcact ccggcggtgg
     1141 cgacgccaaa tccatcgatc gccgtgacaa aatacacccc gccggtggcg cgcgccgtga
     1201 aggcattatt cgcgccacac ggataatcga agttgttcga atcggcccag gtgaagcagc
     1261 cgtcgttata ggatttggcg cgctagccca acgctttcgt gtgtcacccg ctgtgtttgt
     1321 ttaaccattc cacagacaga ctcggagggt ccaagaccct ttgagtctca ctcttcaaac
     1381 gatctaaaac aaaacgcccg atgcatttgg catcgggcgt ttgtgcttca tgatgaaccg
     1441 tttacttgag cacgagcggc aagtagctca acaacgagta gaccggatcg ctgatctgcc
     1501 gctcgaccgc gccggtatcg ccacctaagc cttgcggccg accgatgccg cgctgatcgg
     1561 tgctgggata atcgctgccg gagatcagat cgatcaacgg actgtccggt aaaggcaaat
     1621 gtgtcttgag cgaaccgcct accaagccca gtggattcag tttagccgcg acgctgtttt
     1681 gatcgcccgt gccgctgagt gcacaagtgt tgtcgctgct gagcgaatac tttgacgagt
     1741 caaaggcctt tcccttgcaa ttgcctccgc tagtgctatc ggcaataacg gtattcttca
     1801 aatacgtgtg cgtctccgga t
//
LOCUS       DASRTJ010000492         1591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_69077,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000492 DASRTJ010000000
VERSION     DASRTJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69077"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFF70_10010"
     CDS             <1..462
                     /locus_tag="VFF70_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45070.1"
                     /translation="ALGFVVLIFGVLPGLFLGAVGLSRKLSKATESLKKTFIAYAYVL
                     VPLGLMAWIAFSISFVFTNFSYAWSTASDPLGAGWNLLGTAQIVWTPYITQSIPLLQA
                     SVLLIGLGWSSVTARRIANETQSGRSAMLPALPVVGYCFVITIGLMGLLIG"
     gene            459..1433
                     /locus_tag="VFF70_10015"
     CDS             459..1433
                     /locus_tag="VFF70_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZY45071.1"
                     /translation="MKAERIARVLIVLLAVGLPSLAVIARSSAGAIELHGSIADNGGW
                     SPANLSASVGQPIHLRLTSDDVVHGFAIGQSDQAAIDVMPGVMTETMLIFDQPGKYTF
                     YCTRWCGLNHWRMRGTIEVSGSSSETVASSTPLYIDLKLNIDDPHPAGVVPADKPSAE
                     RGLAVQSSIPLVYLDPKIYQTQSPAVVWRELRIAPFSKNLSDAQVWDVVAFIWKANAT
                     PQAFTAGQKLFAQNCAACHGETGQGNGVMAAALDKGSQASLGESTVRPIDFTNAANML
                     GASSAVLQGKIIRGGMGTGMPYWGPIFTDRQIWSLINYLWSFQFDTLN"
BASE COUNT          342 a          382 c          466 g          401 t
ORIGIN      
        1 gcgcttggct ttgtggtgct gatttttggt gtgttgcccg gtttgttctt gggcgcagtt
       61 ggactgagcc ggaaattatc taaagccact gaatctttga aaaaaacatt tattgcgtat
      121 gcgtatgtgt tagtgccgtt aggcttaatg gcgtggatcg cttttagcat ttcgtttgtg
      181 ttcacgaatt tttcctatgc atggtcgact gcctccgatc cgcttggggc aggctggaat
      241 cttttgggga cagcacaaat cgtgtggaca ccatacatta ctcaatcgat tccactgctt
      301 caagccagtg tgttgttaat tgggttgggc tggtcgagtg taaccgcgcg tcgcattgcc
      361 aacgaaactc aatcaggccg cagcgcgatg ctgccagctt tgcctgtagt tggttattgt
      421 ttcgtgatca ccattggtct gatgggattg ttgatcggat gaaagccgaa cgtattgcgc
      481 gagtgttaat tgtcttgctc gccgtcggcc tgccgtcgct cgccgtgatc gcccgatcga
      541 gcgcgggcgc gatcgaacta cacggatcga tcgcggacaa tggcggttgg agtccggcga
      601 atctttcagc gagtgtaggt cagccgattc atttacgttt gacatcggac gatgtggtgc
      661 acggctttgc gatcggtcaa agcgatcaag cggcgatcga tgtgatgccg ggcgtgatga
      721 ctgaaacgat gctgatcttt gaccaaccgg gcaagtacac attttattgc acgcgctggt
      781 gcggtctcaa tcattggcgg atgcgcggca cgatcgaagt aagtggctcg tcgagcgaaa
      841 cggtggcatc atccacgccg ttatatatcg atctgaaatt gaacatcgac gatccgcatc
      901 cggctggcgt cgttccggca gataaaccat cggcagaacg cggcttggcg gttcaatcga
      961 gcatcccgct ggtttatctc gatcccaaga tttatcagac ccaaagtccc gctgttgttt
     1021 ggcgcgaact tcgaatcgcg cccttttcaa aaaatttaag cgatgcgcaa gtgtgggatg
     1081 tcgtggcatt catctggaaa gccaatgcaa cgccgcaagc atttaccgca ggccaaaaac
     1141 ttttcgcgca gaattgtgcg gcctgtcatg gcgagaccgg gcagggcaat ggcgtaatgg
     1201 ccgccgcact tgataaaggg tcgcaagcga gtctgggcga atcgactgtg aggccgatcg
     1261 atttcactaa tgccgcaaat atgctcggtg caagttcagc cgtcttgcaa ggtaagatta
     1321 ttcggggcgg catgggcacg ggcatgccgt attgggggcc gatctttacc gatcggcaaa
     1381 tttggtcgtt gatcaattac ctatggtcat tccaattcga caccctcaac taaaggagca
     1441 gatatgagca accgtcaact gcgaaaggca gccgcgaaat atcaacagca gcagagacgc
     1501 gcggtgccgt ggtgggcaat cggattggtg gctgccggcg tggtcgtgat cgccgtggtc
     1561 ttgatcggcg gtaacaacaa tttgacgccg g
//
LOCUS       DASRTJ010000493         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_106221,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000493 DASRTJ010000000
VERSION     DASRTJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106221"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1768)
                     /locus_tag="VFF70_10020"
     CDS             complement(284..1768)
                     /locus_tag="VFF70_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY45072.1"
                     /translation="MASNTIFLINFRNHRDMYPPFGIMYVADALEQIGFNVKIFHETE
                     EFVEPFVKQIETERPLFVGFSTITGPQLRPTIEASKKVHALGTPVVWGGVHATIMPLD
                     VLKEDYVDFVVVNEGEVTAQELAQMLSWQKPPLWSSVAGLAYKETDGTPMFHRERDFI
                     EDLDRFTPRWEKIPVERYLIPSGSYTRAIPVYISRGCPFRCGFCYNEVVMKRTWRQHS
                     DEVILRQIQWLKDNYRIDAIDYADDYLFGRIKPMQRLVEKVGMPWSGQVRVQLLTPEF
                     VKWLHDTGCQWVNIGAESGSQAVLDNMHKDQKAWQIEWGMQNLTDGAPNIEANLSFIV
                     GLPGETPADTKITLDLMETLCDMNAKARSSVCVYMPYPGTPLWPQALELGYVPPATQE
                     GWCEMDLNRGNTPWVSDAEAQVMSDIDDILFVGRSKGHWLLTPYYKLLRWRWHHRYFK
                     HYWEGALKRSISNSPLRGILQRATKKLVDFNPITHKGPAADATV"
     gene            complement(1876..>2772)
                     /locus_tag="VFF70_10025"
     CDS             complement(1876..>2772)
                     /locus_tag="VFF70_10025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY45073.1"
                     /translation="RLNTIEKYTQPEVIEFWRRFSQQGLQKCESEMLARYAPPPARMI
                     DLGCGAGRVGIALKDSSYAVTGLDLVWPMIRTARELYAENNIAPNLLQADIRSVPCAN
                     DHYDVALIFIAALQHISGRIARREVFAEVSRIIRSDGVLILALDNLAPALTCYGWWVW
                     RKVRSILKPSSNGHAIDHTAADDLLESRRMNRSTLSWHARGLARTLRWRTWTGLIDRA
                     RQAHLLRGEIGDTAIDQVSLTPTRGMVYYHVYRHAELIDDAASAGLTLLGYHDGRELN
                     EGQTFTPRVRQLDKQVLYAFRH"
BASE COUNT          611 a          822 c          700 g          639 t
ORIGIN      
        1 cactcgctct ctgcggatcg agctgctggg cggctgctgg gccgttcatc gaccacaaca
       61 tggtcatcgc cacgattgcg atgagcagca gcgcagcctt acgtgaaacg aaatgccaga
      121 tattcatgtc agcctcctat atcatctctt atcggccagc atttgatcct gatgcccatt
      181 ggaatcgggt tacttgatca ctcttcccgc cctgattgcc ggccgtgtgt gtgtggcggg
      241 cttgaacttg actcaaaaaa gtttcaaatc tcggcagcac ctattacacc gttgcgtcag
      301 ccgccgggcc tttgtgcgta atcggattga agtccactaa cttcttcgtc gcgcgctgca
      361 atatgccgcg cagcggcgaa ttcgagatcg atcgtttgag cgcaccttcc cagtaatgct
      421 taaagtagcg gtgatgccag cgccagcgca gcagtttgta atacggcgtc agcagccaat
      481 ggcctttcga tcgaccgacg aacaagatgt cgtcgatgtc gctcatcacc tgcgcttcgg
      541 catcgctgac ccacggtgtg ttgccgcgat tgagatccat ttcgcaccag ccctcttgtg
      601 ttgcgggcgg cacgtaaccc aactccaaag cttgcggcca caacggtgtg ccgggatatg
      661 gcatatacac gcacaccgaa ctgcgcgcct tcgcattcat gtcgcacaac gtctccatca
      721 aatccaaagt gatctttgta tcggccgggg tctcaccggg cagcccgacg atgaacgaca
      781 gattcgcttc gatgttgggc gcgccatcag tcaggttctg catcccccat tcgatctgcc
      841 atgctttttg atccttatgc atgttgtcga gcaccgcttg cgatccggac tccgcgccga
      901 tgttgaccca ttgacaaccc gtgtcgtgca gccacttcac aaattccggc gtaagcagtt
      961 gcacgcgtac ttgtccgctc cacggcatgc cgactttttc gacgaggcgc tgcatcggct
     1021 tgatgcggcc gaataaataa tcgtcggcgt aatcgatcgc atcgatgcga tagttgtcct
     1081 tcaaccattg aatttgcctg agaatcactt cgtcggaatg ctgccgccac gtgcgcttca
     1141 tcacaacttc gttgtagcag aagccgcagc gaaacgggca gccgcgtgaa atgtaaacgg
     1201 ggatcgcgcg cgtatacgag ccgctgggga tcaaataccg ttcgaccgga attttttccc
     1261 agcgcggtgt aaatcgatcg aggtcttcga tgaaatcgcg ttctcgatgg aacatcggtg
     1321 tcccatcggt ttctttgtag gcgagtccgg cgacggatga ccacagtggc ggtttctgcc
     1381 aactcaacat ctgcgccaac tcttgcgccg tcacttcacc ttcattgacg acaacaaaat
     1441 cgacgtagtc ttctttcaac acatcgagcg gcatgatcgt cgcatgcacg ccgccccaca
     1501 cgacaggcgt gccgagcgca tgaacttttt tggaagcctc gatcgtcggg cgcagctgcg
     1561 gcccggtgat cgtgctgaag ccgacaaaca atgggcgctc ggtttcaatt tgcttgacga
     1621 acggttcgac aaattcttcc gtctcgtgga aaattttaac gttaaagccg atctgctcca
     1681 acgcgtcggc cacatacatg atcccgaacg gcggatacat gtcgcggtga tttcgaaaat
     1741 tgatgaggaa aattgtattg ctggccatag tctcccccgg ggtggaaatt agaaattgga
     1801 gattggaagt tggaacatac atctgccaat catcatgcga ctaacttcta aaatccaact
     1861 tttaaccttc aacctctaat gccgaaatgc gtacaacact tgtttatcca attgtcgcac
     1921 acgcggcgta aacgtctgcc cttcatttaa ctcacgcccg tcgtgatagc ccaacagcgt
     1981 caaccccgct gacgccgcat cgtcaatcaa ctccgcgtgt cgatacacat gataatacac
     2041 cattccccgc gtcggtgtca gactcacttg atcgatcgcc gtatcgccga tttcaccgcg
     2101 gagcaagtgc gcttgacgtg cccgatcgat caagccagtc cacgttcgcc aacgcaatgt
     2161 tcgagcgaga ccgcgtgcgt gccaactcaa cgtacttcga ttcatgcgcc gcgattcaag
     2221 cagatcgtcc gccgcggtat gatcgatcgc atggccgtta ctcgagggct tcaagatcga
     2281 tcggactttg cgccagaccc accacccata acaggtcagc gccggagcca gattatcgag
     2341 cgcgagaatt agcacgccgt ccgatcgaat gatccgcgag acttcggcaa acacttcacg
     2401 tcgagctatt cgacccgaaa tgtgctgaag cgccgcgatg aagatcaatg ccacatcata
     2461 gtgatcattc gcgcagggaa ctgatcgaat gtcggcttgc agcaaattcg gcgcgatatt
     2521 attttccgca tacagctcac gcgcggtgcg aatcatcggc cagaccagat cgaggccggt
     2581 gactgcgtag ctgctgtcct tcaatgcgat gccgacccga cccgcgccgc agcccagatc
     2641 gatcatgcgc gctggcggcg gggcataacg cgccaacatt tccgattcgc atttctgcaa
     2701 gccctgctga ctgaatcgtc gccagaactc aatcacttcc ggctgcgtgt atttctcgat
     2761 cgtgttcagg cg
//
LOCUS       DASRTJ010000494         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_278490,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000494 DASRTJ010000000
VERSION     DASRTJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278490"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..486
                     /locus_tag="VFF70_10030"
     CDS             76..486
                     /locus_tag="VFF70_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45074.1"
                     /translation="MRPELDQYALEVLQFLAQTGAQVNQLQNVLRQTTGLSGIASFVE
                     SRNYGKDVYLCICLEADVSGERTLTWWMDIRSVVNGWLIEASVLWNGREAVIELPPQV
                     VPDFRAVEKQVPLILGKILEAGTQALVSTVAERK"
     gene            536..1672
                     /gene="hppD"
                     /locus_tag="VFF70_10035"
     CDS             536..1672
                     /gene="hppD"
                     /locus_tag="VFF70_10035"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZY45075.1"
                     /translation="MTLKEMPKIEEKPIDQEDDFLKINAIDHIEFWVGNAKQSNFFWK
                     QWGLKPTAYSGFETGNRRYASYVLEQSQIRFVLTTPYSPHDEMSAHTLLHVDGVKAIA
                     MEVDDVESAYRETTSRGAKGVLAPTETKDEFGMLRAAEIAGYGQTNFRFIDRSDYTGP
                     FRPTYKALPEDMTVKPAGLAAVDHIVGNVELGKMNYWVEWFHKTMGFRQIVHYDDKVI
                     HTEYSALMSKVMGNGNGRIKFPINEPAEGRKKSQIEEYLDYYIGAGVQHIALITGDII
                     ATVRELRSRGLEFLRVPDAYYASLPERVGKIKESYDDIAELGILADRDDEGYLLQIFS
                     RPIQDRPTMFMEVIQRRGARGFGVGNFKALFESIEIEQGKRGNL"
     gene            1751..2740
                     /locus_tag="VFF70_10040"
     CDS             1751..2740
                     /locus_tag="VFF70_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZY45076.1"
                     /translation="MKLVSYFLPNQAEAKLGAIVGDNVIDLVEAEAWAVETDRLAALR
                     LSHEAHDPILALLNTPDGIDQARALLKLLEKEDVRHLHLNFPLNQVRLRAPIGRPPSL
                     RDFYAFEQHVKTARANRGAEMIPDWYDIAIFYFSNHNAIAGPYDSVKRPKASKSLDFE
                     LEVAAVIGRGGIDIPARNADEYIAGYMVMNDWSARDLQMKEMKMNLGPAKGKDFATTL
                     GPNLVTPDELLDKKIDRGDQGSLYNLQMIGCVNGKETSRGNWKDMYFTFAQMIERASA
                     DVQLYPGDVIGSGTVGTGCLLELTKGQGPWLQPGDVVELEIEGLGLIKNQVRE"
     gene            2857..>3367
                     /locus_tag="VFF70_10045"
     CDS             2857..>3367
                     /locus_tag="VFF70_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZY45077.1"
                     /translation="MNQPITFKDIQSAAIRLAGHAHRTPVVTSQTFNDLIGAKVFFKC
                     ENLQRAGAFKFRGAYNTISQLSDNQRSRGVVTFSSGNHAQAVALVAKLLGISAVIVMP
                     NDAPEIKLNATRGYGAEVILYDRVEQKREAIATEIAEERGMALVPPFDHPHIIAGQGT
                     TALELMTDVP"
BASE COUNT          839 a          816 c          963 g          749 t
ORIGIN      
        1 gaatgaacat caacctgaga tcatgcccgc ctgatcgatc gggcggcaga ccgcgatcgg
       61 atatatcgaa ctagcatgcg gcctgaatta gatcaatatg ctttagaagt cctgcaattt
      121 ctagcgcaga caggcgcgca ggtcaatcag ctgcaaaacg ttttgcggca gaccacgggc
      181 ttaagcggca tcgcgtcttt tgtcgaaagc cggaattacg gcaaagacgt ttatctgtgc
      241 atttgccttg aagccgatgt cagcggtgag cgaacgttga catggtggat ggatatccgg
      301 tcggtcgtga atggctggct gatcgaagcg agtgtactgt ggaatgggcg tgaagcggtt
      361 atcgagctgc cgccgcaggt cgtgccggat tttcgtgccg tggaaaaaca agtcccactc
      421 attcttggaa agattttgga agcaggcacc caagcgctcg ttagcacggt cgccgagaga
      481 aaatgatcag actgtgatcc aataactagc gctattcatc ttgggggaga caacgatgac
      541 actaaaagaa atgccgaaaa ttgaagagaa gccaatcgat caagaagatg actttctgaa
      601 gatcaacgcg atcgatcaca ttgagttctg ggttggcaat gcgaagcaat caaacttctt
      661 ctggaagcaa tggggcttga agcccaccgc ctacagcggt tttgaaaccg gcaaccgccg
      721 ctatgcatca tatgtgctgg aacaaagcca gattcgattt gtgttgacca caccctattc
      781 gccgcacgat gaaatgtcgg cgcacaccct gctgcacgtc gacggtgtga aagcgatcgc
      841 gatggaagtc gacgatgtcg aatcggccta tcgggagacg acgagtcgcg gcgccaaggg
      901 tgtactcgca ccgaccgaaa cgaaagatga attcggaatg ctgcgggctg ccgagatcgc
      961 gggttatggc caaacgaact tccgcttcat cgatcgcagc gattacaccg gcccgtttag
     1021 accgacgtat aaagctttgc ccgaagatat gacggtgaag cctgccggtt tggccgcggt
     1081 cgatcatatt gtgggcaatg tcgaactcgg caagatgaac tattgggtcg agtggtttca
     1141 taagaccatg ggcttccgcc agatcgttca ctatgacgat aaagtgatcc acaccgaata
     1201 ttcggcgttg atgtcaaagg tgatgggcaa cggcaatggc cgcatcaagt tcccgatcaa
     1261 cgaaccggcc gaaggccgca aaaaaagcca gatcgaagaa tatctcgatt actacatcgg
     1321 cgccggcgtg cagcacattg cattgatcac cggtgacatc attgcaacgg tgcgcgaatt
     1381 gcgtagccgg ggtttggaat tcctgcgcgt gccagacgcc tattacgctt cgctgcccga
     1441 gcgtgtcggt aagattaaag aatcgtatga tgacatcgcc gaactcggta tcttggctga
     1501 tcgagacgac gaaggttact tgctgcaaat tttcagccgc ccgatccaag atcgaccgac
     1561 gatgttcatg gaagtgatcc agcgccgcgg tgcgcgcggt ttcggcgtcg gcaatttcaa
     1621 ggcgctattc gaatcgatcg agattgagca gggcaagcgt ggcaatctgt gatcagcgat
     1681 aagttaatca gggattaggg atcaggtgta ggggcgaggt tacctcgccc ttatcatgta
     1741 aggagttgag atgaaactcg tttcatattt tctaccgaat caggccgagg caaaactcgg
     1801 cgcaatcgtc ggcgacaacg tgattgatct agtcgaagcc gaggcgtggg ctgtggaaac
     1861 cgatcggttg gcggcgctcc gattgtccca cgaggcccac gatccgatct tggcgctgct
     1921 caacacgccc gacgggatcg atcaagcgcg cgcgttattg aagttattgg agaaagaaga
     1981 tgttcggcat cttcatctaa attttccact aaatcaagtt cggttgcgtg cgccgatcgg
     2041 tcgtccgccg agcctccgag atttctacgc gttcgagcag catgtgaaga cggcgcgagc
     2101 caatcgcggc gcggagatga tcccggattg gtacgacatc gccatctttt atttttcgaa
     2161 tcacaacgcg atcgctggtc cctacgattc agtgaaacgg ccgaaagcct cgaagtcact
     2221 ggattttgaa ttggaggtcg cggcggtgat cggcagaggc ggcatcgata ttcccgcgcg
     2281 caatgccgat gaatatattg ccggttacat ggtgatgaat gattggtcgg cgcgcgatct
     2341 acaaatgaaa gagatgaaaa tgaatctcgg tccggccaag ggcaaagatt ttgcaacaac
     2401 gctcggtcca aatctcgtca caccggatga acttcttgat aagaagatcg atcgaggcga
     2461 ccaaggctcg ttatacaatt tgcaaatgat tggctgcgtc aatgggaaag aaacatcgcg
     2521 tggcaattgg aaagatatgt acttcacctt cgcgcagatg atcgagcgcg catcggcgga
     2581 tgtgcagctg tatcccggcg atgtgatcgg ctcgggcact gtcggcaccg gctgcctgct
     2641 cgaactcacg aagggtcaag gcccgtggct tcagcctggc gatgttgtcg agttggagat
     2701 tgaaggtttg ggtctcatta agaatcaagt gagagaatga tctgacattt cgagcgcagc
     2761 gagaaatctc tataatgatt ggcggcggtc gctaatgggg tagagatttc tcatcgcgtt
     2821 tagcgcgctt cgaaatgaca aaacaaagaa aactttatga atcagccaat caccttcaaa
     2881 gatattcaat ccgcggccat tcgattggca ggccacgcgc atcgaacgcc agtcgttact
     2941 tcgcaaacgt tcaatgatct cattggcgcg aaagttttct tcaagtgcga gaatttgcag
     3001 cgcgcaggcg cgttcaagtt tcgcggcgcg tataacacga tcagccaact gagcgacaat
     3061 caacgttcgc gcggcgtggt gacgttttca tctggcaatc acgcgcaggc ggtggcgttg
     3121 gtcgcgaagt tgctaggtat ttcggccgtg atcgtcatgc cgaatgatgc gcccgaaatc
     3181 aaattgaatg cgacgcgggg ttatggcgct gaagttattt tgtatgatcg cgttgaacaa
     3241 aagcgcgaag cgattgcaac agaaattgcc gaagagcgtg gcatggcact cgtgccgccg
     3301 ttcgatcatc cgcatatcat cgccggacaa ggtacgaccg cgctggagtt gatgaccgat
     3361 gtccccg
//
LOCUS       DASRTJ010000495         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_258770,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000495 DASRTJ010000000
VERSION     DASRTJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258770"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFF70_10050"
     CDS             <1..272
                     /locus_tag="VFF70_10050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY45078.1"
                     /translation="VVIVRHVFNDGRTILSFYGHLDPPSVVLNVGDCVERGQQVGEIG
                     KPRGRPHLHFEIRHHLPARPVPATGRSIQLWRVGNRRASTSGTIA"
     gene            269..1276
                     /locus_tag="VFF70_10055"
     CDS             269..1276
                     /locus_tag="VFF70_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZY45079.1"
                     /translation="MSTAPGVDWLRAFTATDSIGAGVLSNTLITYANDKLLAVNAEDG
                     QLKWSQPLTGLYQTLIDVNGTALYASTLSGDVQSLDSSGTLNWQMDFDEFDRLNLMPL
                     PGGGVIVQIDQQLIGVSAAADRLWQIDQIAAPFDWLRQGDRLLFSTSGDRSATYSIDR
                     SGRVIKLAEIGGRLAFSHDRLFIYTSNGVYRLSATPPLVDLVLPLDRDVFNTGRIITT
                     EDGTLLITHHGLTDRRLIALNSDGSLRWDRSVLDLGSDLPYLMTVDQQVYAITLDGDV
                     LRIDPQTGAARRLFNGGPLLPLSGKPWGVAANGRLIFDFRGGTLVGFDPRAVDQIDLG
                     E"
     gene            complement(1346..>1788)
                     /locus_tag="VFF70_10060"
     CDS             complement(1346..>1788)
                     /locus_tag="VFF70_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZY45080.1"
                     /translation="RDMPDLERKEIETIFSNWGFESDLLSRSVEQVMKDERAWVDVMI
                     HNELKLAPVQASTALRSALIVGLAAIVGSLIPMFPFFFYPINTGIPIAIVITAITLFG
                     VGVYKARTMVGHPVKSGLQMAVIGTLSALAGYLVGSLFSAPPTP"
BASE COUNT          362 a          488 c          527 g          411 t
ORIGIN      
        1 gcgttgtgat cgtccggcat gtgtttaatg acgggcgcac cattctgtca ttctacggac
       61 atctcgatcc gccgagcgtg gtgttgaacg tcggcgattg cgtcgagcgc ggtcagcagg
      121 tgggtgagat cgggaaaccg cgcggccggc cacatttgca tttcgagatt cgacatcatc
      181 tgcccgctcg cccggtcccg gctactggcc ggtcgatcca actttggcgg gttgggaatc
      241 gccgagcgtc tacatctggc acgatcgcat gagcaccgcg cccggtgtag actggttgcg
      301 cgcgttcacc gccactgact cgatcggcgc gggtgtactc agcaatacat tgattactta
      361 tgcgaacgat aaattgttgg ccgtgaatgc ggaggacggt caattgaagt ggagccagcc
      421 actgaccggg ctgtatcaaa ctctgatcga tgtgaacggg actgcgcttt acgcttcaac
      481 actttctggc gacgtgcagt ccctcgatag ttcgggcacg ttgaactggc aaatggattt
      541 tgatgaattc gatcggctca acttgatgcc tttgcctggc ggcggagtga tcgttcagat
      601 cgatcaacag ctgatcggcg tttcagccgc agccgatcgg ttgtggcaga tcgatcagat
      661 cgctgcgccg ttcgattggc tgcggcaagg cgatcgattg ttgttcagca ccagcggcga
      721 tcgatcggcg acgtactcga tcgatcgatc cggccgagtg atcaagctgg ccgagatcgg
      781 cgggcgcttg gccttctcgc acgatcgatt gttcatctac acatccaacg gtgtttatcg
      841 attgagtgcc acgccgccgt tggtcgatct cgtcttaccg cttgatcgcg atgtgttcaa
      901 caccggtcgc atcatcacca ctgaagatgg cacactgctc attacgcatc acggcttaac
      961 cgatcgacgt ctcatcgcgc tgaattccga tggcagtctg cgctgggatc gatcggtttt
     1021 agatttgggc agtgacttac catatttgat gacagttgat caacaagtct atgcgatcac
     1081 gctcgatggc gatgtcctgc ggatcgatcc gcagactggt gcggcgcggc ggctgttcaa
     1141 tggcggaccg ctgctgccgc tatcggggaa accgtggggc gttgcggcaa atggccgctt
     1201 gatttttgat tttcggggtg gcacgctcgt cgggttcgat ccgcgcgcgg tggatcagat
     1261 cgatctgggg gagtaagttg ataaatgcac aaccgggttg cttcaagcaa tccggtttca
     1321 ttttaggcgc tgataaacaa atcactcacg gagtcggcgg cgcactgaat aatgatccga
     1381 cgagataccc ggccagcgcg gacagcgtgc cgatgaccgc catctgcaag ccgctcttaa
     1441 ctggatgccc gaccatggtg cgcgctttgt acacgccgac gccgaacaag gtgatcgccg
     1501 tgatgacgat cgcgatcgga atgccggtgt taatgggata gaagaaaaat ggaaacatcg
     1561 gaatcagcga gccgacgatc gcggccaagc cgacgatcaa cgcgctgcgc aaggcggtgc
     1621 tggcctgcac cggcgcgagt ttcaattcat tgtgaatcat cacgtccacc cacgcgcgct
     1681 cgtccttcat cacttgctca accgatcgag ataatagatc gctttcaaag ccccaattgc
     1741 tgaagatcgt ttcgatctcc ttgcgctcca gatcgggcat gtcacgga
//
LOCUS       DASRTJ010000496         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_278590,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000496 DASRTJ010000000
VERSION     DASRTJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278590"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFF70_10065"
     CDS             <1..502
                     /locus_tag="VFF70_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZY45081.1"
                     /translation="GQGYMQVTPLQLARMYSAIANGGTLYRPEVILKIGSNNEPPEET
                     FQPDIQSQLPISPENLKSIQNGLHGVISSPRGTAGFVFRGFPHSVAGKTGTAETGGPN
                     DVSHAWFVAYAPFEDPQILVVVFLENGGQGSYNAAPLARNVLEAYFNLSFTPPPTTPP
                     SGPEDR"
     gene            646..1953
                     /locus_tag="VFF70_10070"
     CDS             646..1953
                     /locus_tag="VFF70_10070"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HZY45082.1"
                     /translation="MAANPFTYGNPISDPARFFGREREIEQVFSRLRNAEFESSSLVG
                     ERRVGKTSLLNAIAHPDIRHQHGLDPAQYIFVYVDLEMVDKDATPLKLWQRMLRQMAR
                     ACQDAEVKQAIEEISKENAIDTFALADLFDSIEEKKQYVVFLLDEFENVTTNQNFGPD
                     FFYGLRSLAIQHNLALITASRRELIELCHSDAIRSSPFFNIFANINVGLLNADETRLL
                     IARSMEGTGITLSEDEVNLICRLAGYHPYFLQAACYFLFEAYSKNLPSEERRTYLLKE
                     FRQEANPHLADYWYNSDDHEKIVLTVLALLERQGKANGRSFSMKQLQEYYTRSDQTLS
                     HLEKRSLISAQADGYMLFNTLWGEWIMTEITDIPKDAQSYDDWLKSNQSALGKLSGKA
                     KSEIGEILPKISSKYRDLIVGWVSDPKNWVAVLGLLKGVLLPV"
     gene            1963..>3371
                     /locus_tag="VFF70_10075"
     CDS             1963..>3371
                     /locus_tag="VFF70_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY45083.1"
                     /translation="MSEIVNPYIAGNPVTGTEMFFGREDVFTFVQQALVGQHRDNVIV
                     LYGQRRTGKTSVLYQMHRHLDPRYLCIFIDLHGLALEGINGFLWELASTITRALRKDH
                     QIELPRPKERADFIADARSSFENDFLPQVWSAIGDRHILLMLDEAIRLQEQIQAGKLE
                     PEVFEYLRHLMQHHERLDFLFSLGSGLEEMEKEYAFLFSVGLYKKISFLERAPAIALI
                     TEPVKDCYHVEPTAIDRILQITSGHAYYTQLVCHSLLNRWQQDHRAQITAKDVDAILD
                     EVVERGLAVLKHVWEESTSGEKAVLAGLAAIMGDHNRPIGPQEIDRGWAQHTVILPES
                     ERSKAIQSLIARDVIAGRDKYEFAIELQRIWVAKYERMEWVKEEIASDVQQWQSQPGV
                     LTKPRSFLRTRTGLIAAASISAVFLILVILLLQAIIPPGPTAPSTIAANTGNNTSNTN
                     PGGAAANVSALTIVDNIVWT"
BASE COUNT          823 a          894 c          895 g          759 t
ORIGIN      
        1 cggtcaaggc tacatgcagg tgacgccgct ccaacttgcg cggatgtact ccgcgatcgc
       61 gaatggcggc acgctctatc gaccggaggt catcctcaag attggatcga acaacgagcc
      121 gccggaagaa acttttcaac cggacattca gagccaactt ccgatctcgc cggaaaattt
      181 gaagtcgatc caaaatggct tgcatggtgt catcagcagt ccgcgcggca ctgctggctt
      241 tgtctttcga ggttttcctc attcggttgc cggcaagaca ggcacggcgg aaaccggcgg
      301 tccgaacgat gtctcgcatg cctggttcgt cgcttatgcg ccgttcgaag atccgcagat
      361 cctagtcgtt gtctttctcg aaaacggcgg gcaaggatcg tataatgctg cgccgctcgc
      421 acgtaatgtt ttggaggcgt atttcaatct ctcgtttact ccgccaccga ccacaccgcc
      481 gagcggaccg gaagatcgat aggcgctaca acgcacgagg cgtgcgatgt acatcacgcg
      541 tcttgtttgc tttgtatctt cgattcgcgt acaattcaag caaatctcgt cttcgatcgc
      601 tccagccgca aatgtgatct cattaacgag aaagcgatct cgactatggc tgccaatcca
      661 tttacctacg gaaatcctat atctgatcca gcacgattct tcgggcgcga gcgtgagatc
      721 gagcaggttt tcagccgcct gcgtaatgcc gaatttgaat caagttcgct cgtcggcgag
      781 cgccgggttg gtaaaacttc gctcctgaac gccattgccc atcctgacat ccgtcaccaa
      841 cacggactcg atcccgctca atacatcttc gtgtatgttg atctagaaat ggtcgataaa
      901 gatgcgacgc cattgaaact gtggcagcgt atgttgcgac aaatggcgcg tgcttgccag
      961 gatgccgagg tcaaacaagc cattgaagag attagtaaag aaaacgcgat cgatactttc
     1021 gcgttggctg atctcttcga cagcatcgaa gaaaagaaac aatacgtcgt ctttctgctc
     1081 gacgaatttg agaacgtcac gacgaatcaa aacttcggac cggatttttt ctacggcctg
     1141 cgcagtctcg caattcaaca caacctcgct ctgatcacgg ccagccgccg cgaactgatc
     1201 gagttgtgtc actcagatgc gatccgatca tcgccgtttt tcaacatctt cgccaacatc
     1261 aacgtcggcc tactcaatgc cgatgagact cggctgttga tcgcgcgatc gatggaagga
     1321 acaggcatca cattgagtga ggacgaagtc aacttgattt gccgcctcgc cggttatcat
     1381 ccctactttt tgcaagctgc ctgttatttc ttattcgagg cttattcaaa aaacttaccg
     1441 tctgaagaac gacgcacgta tctgctgaag gagtttcgcc aggaagccaa tccacatttg
     1501 gccgattact ggtacaactc cgatgatcac gagaagatcg tactcacagt cttggctttg
     1561 ctggagcggc agggcaaagc caatggacga tcgtttagca tgaaacagct gcaagaatat
     1621 tacacgcgat ccgatcaaac gctatcccac ttggagaaac gcagcctgat cagcgcacag
     1681 gccgatggtt atatgttatt caacacgttg tggggcgagt ggatcatgac tgagatcacc
     1741 gacataccca aggacgcgca aagctacgac gactggctta aatccaacca aagtgcgttg
     1801 gggaaattat cgggtaaggc caagagcgag atcggtgaga tcctgccgaa gatcagcagc
     1861 aagtatcgcg atttgatcgt cggctgggtg agcgatccaa agaactgggt ggccgtcctc
     1921 ggcttgctca agggtgtatt gctgccggta tgaggacaaa ccatgtctga aatcgtcaat
     1981 ccatacatcg cagggaatcc cgtaaccggt acagaaatgt tctttggccg cgaggacgtg
     2041 ttcaccttcg tgcagcaagc gctagtcgga cagcatcgcg ataacgtcat cgtactctac
     2101 ggtcagcggc gcacgggcaa aacgtcggtg ctgtatcaaa tgcaccgtca tctcgatccc
     2161 cgctacttgt gcatcttcat cgatctgcac ggcctcgcac tggaagggat aaacggcttc
     2221 ctgtgggaac tcgccagcac gatcacgcgc gcattacgca aagaccatca gatcgaactg
     2281 ccgcgcccga aagagcgcgc ggactttatc gctgatgcgc gcagctcctt cgagaatgat
     2341 tttctgccgc aagtgtggtc ggcaattggc gatcgccaca tcttgctcat gctcgacgaa
     2401 gcgatccgac tgcaagagca aattcaagcc ggcaagcttg aaccagaagt gtttgaatat
     2461 ctgcgccact tgatgcagca tcacgaacgc ttggatttcc tgttctcgct cggtagcggg
     2521 ctggaggaga tggagaagga atacgccttc ttattcagcg tcggcttgta caagaaaatt
     2581 tcgttcctgg agcgcgcacc ggcgattgcg ttgatcacgg agccggttaa agattgctat
     2641 cacgtcgagc cgactgcgat cgatcggatt ttacagatca cttcgggcca cgcgtattac
     2701 acgcagctgg tctgccacag cttgctcaat cgctggcagc aggatcatcg agcacaaatc
     2761 acagccaaag atgtcgatgc cattttagat gaagtcgtcg agcgcggcct cgcggtgttg
     2821 aagcatgtgt gggaagagtc aacgtcgggt gaaaaagcag tgctggctgg actggcagcg
     2881 atcatgggtg atcacaatcg accgatcggg cctcaggaga tcgatcgggg ctgggcacag
     2941 cacaccgtga tactgccgga gagcgaacga tcaaaagcaa tccaaagcct gatcgcgcgt
     3001 gatgtgattg ccggtcggga caagtacgaa ttcgcgattg agctacagcg gatatgggtc
     3061 gcgaagtacg agcgtatgga atgggtcaag gaagaaatcg cgagtgatgt tcagcaatgg
     3121 caatcgcagc caggcgtgct aaccaagcca cgatcgtttc tacgcacgcg cactggattg
     3181 atcgctgcgg catcaatctc tgccgtgttc ttgattctcg tgattctttt gctgcaagcg
     3241 atcatcccgc ctggcccaac ggctccaagc acaattgcgg cgaatacggg caacaatact
     3301 agcaacacaa atccaggtgg tgcagccgcg aatgttagcg ccttaacgat tgttgataac
     3361 atcgtttgga c
//
LOCUS       DASRTJ010000497         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_106711,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000497 DASRTJ010000000
VERSION     DASRTJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106711"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..280)
                     /locus_tag="VFF70_10080"
     CDS             complement(38..280)
                     /locus_tag="VFF70_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HZY45084.1"
                     /translation="MVTLFDAGSGSRVGRITEKQLEALIDWMEEESSEDREFYLTGED
                     CELMLEQGIDPTLVEALQAALHGREDMDIRYEKEEQ"
     gene            complement(338..1612)
                     /locus_tag="VFF70_10085"
     CDS             complement(338..1612)
                     /locus_tag="VFF70_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY45085.1"
                     /translation="MTNLLSSDVTQRSMLGKWLRTQTETIAGPWIAAIHSRRSATEQP
                     ATPAIDQSIYQKLYQNLALALETGQYASLDESLTEITIAGRAQGYQLQDLIQIATMLK
                     NDIWQALVTAYPPEETLGYLHTIDILFQTALSYQTRVFSDLMQREAIQTLGLARDELE
                     KVNETKSRFINIAAHELKTPLTLIQGYTDILSQELSDLADEQVLHILGGLRNGARRLL
                     ATVNDMIVVSMIDARTLTLNYQIVALPHIIQMVLNELRPTLRERDLTIVFKPVPIDLG
                     PFYADPQRIYQVLDYIVTNSIKYTPDGGRITIELKLIPGTDPFVEVCVVDTGIGIAPE
                     DLPYIFEKLYGKTDLSKHSSSRTNFKGGGVGLGLSVVKGIVDAHGGKIAIESTGYDEQ
                     KFPGTTVRIFLPNLAQPPAREQSPFGLESRSS"
     gene            complement(1609..2436)
                     /locus_tag="VFF70_10090"
     CDS             complement(1609..2436)
                     /locus_tag="VFF70_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZY45086.1"
                     /translation="MISPIATPAPSIFDGNAAYQHVIAQVGLGARPTGSAALQKTGDY
                     IIEQLRSIGWQVETQEFDYKNTHTRNIIAKRGSGPIIIIGAHYDTRMTADQDPDPGKR
                     NQPVMGANDRASGVAVLLELARTLNIENINREVWLTFFDAEDNGEIDGWDWGVGSTYM
                     ADHLTISPTAMILLDMIGDANLNISWEQNSNPALNQSIWDIAATLGYSQSFLARPGWT
                     MIDDHLAFIRHGIPSVDLIDFDYPYWHTTQDTADKVSPVSLERVGRTLQTWLEQSKP"
     gene            complement(2578..>2765)
                     /gene="gid"
                     /locus_tag="VFF70_10095"
     CDS             complement(2578..>2765)
                     /gene="gid"
                     /locus_tag="VFF70_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="TrmFO; Gid; glucose-inhibited division protein;
                     similar to GidA; the gene from Bacillus subtilis encodes a
                     tRNA-methyltransferase that utilizes folate as the carbon
                     donor and bound flavin as reductant; modifies tRNA at
                     position 54 (uridine) of the T-psi loop to form a
                     C5-methyluridine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZY45087.1"
                     /translation="LGALCHYITHAESNNFQPMKANFGIMPSLENAPHGKRDRARAFS
                     QRALIDLDRVLADRVFA"
BASE COUNT          643 a          721 c          701 g          700 t
ORIGIN      
        1 gagtaatgcg tagtgcgtaa ggtggggggt taactgatca ctgttcttct ttttcgtaac
       61 ggatatccat atcttcccgt ccatgcaggg ccgcctgcag tgcttcgacc aatgtcgggt
      121 cgatgccctg ctctaacatt aactcgcaat cctcgccggt taaatagaat tcgcgatctt
      181 cactgctctc ttcttccatc caatcgatca gcgcctctaa ctgcttttca gttatgcgcc
      241 ctaccctcga gccggatccg gcgtcaaaca aggtgaccat gacagcctcc gcgattgata
      301 aatgctcatc gattataacc tgcacgcgat caaagcgtca agacgatctt gattccaggc
      361 caaacggtga ctgctcacgc gcgggcggct gagctaagtt tggcaaaaag atgcgcacgg
      421 tggtgccggg aaatttctgt tcgtcatagc ccgtactctc aatcgcgatc ttgccgccat
      481 gcgcatcgac gatacctttc accacagata agccgagacc gacgccgccg cctttaaaat
      541 tggtgcggct gctggaatgc ttggacagat cggtcttgcc gtacaatttt tcgaagatat
      601 agggcaaatc ttccggcgcg atgccgatgc ccgtatcgac cacgcagact tcgacgaacg
      661 gatcagtgcc ggggatcagc tttaactcga ttgtgatgcg acccccatcg ggcgtgtatt
      721 tgatgctgtt cgtcacgatg tagtcgagga cttgatagat gcgttgtgga tcggcataaa
      781 aggggccgag atcgatcggg accggcttga agacgatggt taagtcgcgc tcccgcagcg
      841 tcggtctaag ttcattcaac accatttgaa taatatgcgg cagcgccaca atttgataat
      901 tcaacgtcag cgtccgcgca tcgatcatcg acacgacgat catgtcgttg acggtcgcca
      961 gcaagcggcg agcaccgttc ctcaatccgc cgaggatatg gagcacttgc tcatcggcca
     1021 agtccgacaa ttcttggctg agtatgtctg tatatccttg gatcaatgtt aaaggtgtct
     1081 tcagttcatg ggctgcaatg ttgataaagc gcgacttggt ctcatttact ttttccaatt
     1141 cgtcacgcgc cagaccgagt gtctgaatgg cctcacgctg catcaaatca ctgaaaacgc
     1201 gcgtttgata ggatagcgca gtctgaaata gaatatcgat cgtatggaga tagcccagcg
     1261 tctcttcggg cggataagct gtgacgagcg cctgccaaat atcatttttt agcatcgttg
     1321 caatttggat cagatcttgc aactgataac cttgggcgcg tcccgcgatg gtgatctcag
     1381 tcaggctttc atccaaagag gcgtactgcc cggtttccag tgcgagcgct aagttttgat
     1441 acaacttctg atatatcgac tgatcgatcg ccggcgtcgc aggctgctcg gtagcggagc
     1501 ggcgcgaatg aatggcggcg atccatggac ctgcaatcgt ttcagtttgc gtgcgcagcc
     1561 acttgcctaa catcgagcgt tgggtcacat cggagctcaa aaggttggtc atggttttga
     1621 ttgttccagc caggtctgta aagttcgtcc gactcgctcc agactgaccg ggctgacttt
     1681 atcagctgtg tcttgcgtcg tgtgccagta tggataatcg aagtcgatca gatcgaccga
     1741 cggaatgccg tgtcgaatga acgccagatg atcatcaatc attgtccagc ctggtcgagc
     1801 gagaaacgat tgcgaataac caagcgtagc cgcaatatcc caaatgcttt ggttcaatgc
     1861 tggattagag ttttgttccc acgagatatt gagattagca tcgccaatca tatctaataa
     1921 gatcatcgcc gttggggaga ttgttaaatg atcggccatg taggttgaac ccacgcccca
     1981 atcccagcca tcgatctcgc cgttgtcttc cgcatcgaaa aaggtcaacc acacctcacg
     2041 gtttatattc tcgatattca gcgttcgcgc aagttccagc aagactgcga cgccgctggc
     2101 acggtcattc gcgcccatca ccggttgatt gcgcttcccc ggatcgggat cttgatcagc
     2161 cgtcatgcgc gtatcgtaat gcgcgccgat gatgatgatc ggtccgctcc cgcgtttggc
     2221 gatgatgttg cgcgtatgag tattcttgta atcgaactcc tgcgtctcca cctgccaacc
     2281 gattgatctg agctgctcga tgatatagtc gcccgtcttt tgaagtgcag ctgaaccggt
     2341 cgggcgcgca cccaaaccca cttgagctat cacatgttga taggctgcgt tcccgtcaaa
     2401 gatcgacggt gcaggtgtgg caatcggtga aatcaatcgc cctgccccgg tgcaatctgt
     2461 cagaaaaaaa attgaaattg ccgcgatcga aacggagagc aggccgcgta aatttcgcat
     2521 agcttcttat attctaaaga aaataaaatc aggatttatc cacgtatttt gaagtgtcta
     2581 cgcgaatact ctatctgcca acaccctgtc caaatctata agtgcgcgct gactaaacgc
     2641 tcgtgcccga tcacgtttgc catgaggagc attttcaagt gaaggcatga tgccgaagtt
     2701 tgctttcatc ggctgaaaat tatttgattc agcatgcgtg atgtagtgac acagcgcgcc
     2761 cagca
//
LOCUS       DASRTJ010000498         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_31584,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000498 DASRTJ010000000
VERSION     DASRTJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31584"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFF70_10100"
     CDS             complement(<1..586)
                     /locus_tag="VFF70_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZY45088.1"
                     /translation="MTDSIDFSAEVRNQKLRWSFPQPIAIDPELAMAIGGQAIVAELL
                     TRRGLGSIATALAFLDPDRYTPAPPIELPDLEAAADRLYRAITRGEKIAVWGDFDADG
                     QTSTTLLYSLLRDLGANAIYHIPNRLKESHGIKIDSLNRLIDQHVSVLLTCDTGIAEH
                     AAIAHAKQNGLTVLVSDHHDLPDELPNADALINPK"
     gene            complement(583..1701)
                     /locus_tag="VFF70_10105"
     CDS             complement(583..1701)
                     /locus_tag="VFF70_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45089.1"
                     /translation="MPKYIGAIRKPVKRANAERYLLIMLLSFAASVILTRIYLQLTGF
                     PQIGTGELHIAHMLWGGLLLFVSAVLMLTLANSWALNLSAILSGAGVGLFIDEVGKFI
                     TKTNDYFYPVAAPIIYAFFLLTVMFYLHVRGRASSDARSELYHAFENLGEAVDDDLDV
                     YETANIQVRLQRVVAAKDDYPDQARLAQALLDLLHSNQITLVEHQPTRWEKIAKSIKA
                     FEDKYFIRRAHKTLLMIGLGAFGIGSLIALAPVILALTAPIYLEQISQQIIAAGLVSN
                     PSGLIWFTTRLTLQGMVGALLLGSAVFLLFGRDQRGIALGYFGLLLYLLTVNLLVLYF
                     DQFTTIVVTLIQFGLFIGVIRYRQRFLDPQPAPITAHQ"
     gene            complement(1796..>2285)
                     /locus_tag="VFF70_10110"
     CDS             complement(1796..>2285)
                     /locus_tag="VFF70_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZY45090.1"
                     /translation="ISIPLHKYRLTAHLDLIAADAERIVIVDWKTERKRPTRDQLLKR
                     PQTRVYRYVVSEAYERPPGTISMIYWFAEFPDQLEVLQYDAAQHAKDRDLLNQVLVDI
                     ETRAQADGEWDKTPDERKCAYCTYRSLCNRGRSAGVIDPVAEDYDREFSLSQEEIEEI
                     AY"
BASE COUNT          551 a          562 c          653 g          519 t
ORIGIN      
        1 gtttgggatt gatcagggca tcggcgttgg gcaattcatc aggcagatcg tggtgatcgg
       61 atacgaggac ggtcagtcca ttttgttttg cgtgtgcgat tgcggcgtgt tcggcgatac
      121 ccgtgtcaca agtaaggagg acgctgacgt gctgatcgat caaacggttg agtgaatcga
      181 tcttgatgcc gtgcgattct ttcagccgat tggggatgtg gtagatcgca ttcgcgccga
      241 gatcgcgcaa taatgaatag agcagggtgg tggacgtttg tccgtccgca tcgaagtcgc
      301 cccagactgc aatcttttcg ccgcgcgtga tcgctcggta caaacgatcg gcggccgctt
      361 caagatcggg cagctcgatc ggcggggcag gagtgtatcg atcgggatca agaaaagcca
      421 gcgcagttgc gatcgatcct aagccgcgcc gcgtgagcaa ttccgccaca atcgcctggc
      481 cgccgatggc catcgccagc tcgggatcga tcgcgatcgg ttgtggaaag gaccagcgta
      541 atttttgatt gcggacttcc gcagaaaaat cgatcgagtc ggtcattgat gcgcagttat
      601 cggagcgggc tgcgggtcca aaaatctttg gcgatagcga atgacgccga taaagagtcc
      661 aaattggatc aaggtcacga cgatggtggt gaattgatcg aaatagagga caagcaaatt
      721 taccgtgagc agatatagca ataggccgaa gtagcctaag gcgatgccgc gctgatcgcg
      781 gccgaataat aaaaatactg ccgaacccag cagcagtgca ccaaccatgc cttgtaatgt
      841 taagcgcgtg gtaaaccaga tcaaaccaga cggattactg accaggccag cggcgatgat
      901 ttgctgactg atctgttcca gataaatcgg cgcggtgagc gcgagaatga ccggcgctaa
      961 ggctatcaac gatccaatgc caaacgcgcc tagcccaatc attaaaagag ttttgtgcgc
     1021 acgtcggata aaatatttat cttcgaaggc tttaatcgac ttcgctattt tttcccagcg
     1081 ggtgggttga tgctcgacga gggtgatttg attggagtgg agtaaatcga gcagcgcctg
     1141 cgccagccga gcttgatcgg gataatcatc tttggcggcg accacacgct gcaaacgaac
     1201 ctgaatgttg gccgtctcgt atacatccag atcatcgtcg acggcttcgc cgagattctc
     1261 aaaggcgtga tacaactcgc tgcgcgcatc gcttgatgcg cgcccgcgca catgcagata
     1321 gaacataacg gtcaatagaa agaacgcata gatgatcggc gcggcgaccg gataaaaata
     1381 atcgttggtc ttggtgatga atttgccgac ttcgtcgatg aacaaaccga cccctgcacc
     1441 gcttaagatc gccgagaggt tgagcgccca agaattggcg agcgtcagca tcaggacggc
     1501 tgagacaaat agcagcagac cgccccacag catgtgcgca atgtggagct cgcctgtgcc
     1561 gatttgtggg aagccggtta gctgcaaata gatgcgcgtc aaaataactg acgcagcaaa
     1621 acttaataac atgatcagca aatagcgttc cgcgttggcg cgcttaacag gtttgcgaat
     1681 tgctccgatg tatttgggca taacgattac ctccgttagg gcgtagtctg cgaattgaat
     1741 attgagtatt acgtggcgcg ggttccgtta cgcaccaagc aacacacatt atgcatcaat
     1801 acgcaatctc ttctatttct tcttggctga ggctgaattc tcgatcgtaa tcctcggcca
     1861 ctggatcgat cacgccggcg cttctgccgc gattgcacaa cgatcggtaa gtgcagtacg
     1921 cacacttgcg ctcatcgggt gttttgtccc attcgccatc ggcctgggcg cgcgtttcaa
     1981 tatcaacgag cacttgattt aacagatcgc gatctttcgc atgttgggcg gcatcgtatt
     2041 gcaacacttc cagttgatcg ggaaattcgg cgaaccagta gatcatcgag atcgtgccag
     2101 gtgggcgttc atacgcttcg ctgacaacat agcgatacac gcgcgtctgc gggcgcttga
     2161 gaagttgatc gcgtgtcggg cgtttgcgtt cggtcttcca atcgacgatg acaatccgtt
     2221 cggcatcggc ggctatcaga tcgaggtgcg cggtgaggcg atacttatgg agcggaatcg
     2281 aaatc
//
LOCUS       DASRTJ010000499         5883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_126816,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000499 DASRTJ010000000
VERSION     DASRTJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126816"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFF70_10115"
     CDS             complement(<1..347)
                     /locus_tag="VFF70_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZY45091.1"
                     /translation="MKFQKIKFYFIGLICLASLILSACGAPSTATPPSTAVASSVKNI
                     SPIVNATGTVLPAKWTTLSFGQAGTVIDPLVKDGDVVKAGDLIARLAAPDLRANLAQK
                     QAAIKAAEANLSLV"
     gene            complement(600..1343)
                     /locus_tag="VFF70_10120"
     CDS             complement(600..1343)
                     /locus_tag="VFF70_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45092.1"
                     /translation="MANVKSKYNTPANNNRNDDGTLIKLHNIVKTYETPAGKFEALKG
                     IDLSIGSGEFVSIIGKSGSGKTTLINMLTGIDRPTKGEIHIGGMAVHTLNEGQIATWR
                     GSHMGVVFQFFQLLPTLTVLENVRLPMDFCNIYTPQERNERAMYLMELVGIAEHAYQL
                     PNHLAGGQQQRAAIARALANDPPIVATDEPTGNLDSKTAESVMQLFKNLVADGKTILM
                     VTHDTDLANEAERTIVLADGEIVNEYRNN"
     gene            complement(1444..3852)
                     /locus_tag="VFF70_10125"
     CDS             complement(1444..3852)
                     /locus_tag="VFF70_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZY45093.1"
                     /translation="MELSTRWIKILKDLWDHKSRSILVILSIAVGVGAVGMINNAKGI
                     IERDLFESYAAGNPALVQIFVSTFDKSLATAVAGMPEVADAQAKRLATETVYRVDGKS
                     YDINLIAVPDFSDLRIDKYHVEAGAAMPGVREILLERQSAVGLGVTVGDQIMIEVNNQ
                     RRYPVMVVGIVHSLILRPFNLGHQASGYVSMDTLAWMGLGTTYNRLDIITADDPLSRA
                     HDLAVAGLARDRIIQRSGYTVNRIQIPGFSADPGQHWAQNQIIGMLLVLQVMGILVIF
                     LTGGLVVNTISAIMSQQIKQIGIMRSIGAVRRQIIGMYVLNVVVLSVLGLLIGLPLGV
                     IGASGLASLAGNIMNFDISQVTLPPNIALVQIFVGLLMPIGVALYPIISGTRISVYDA
                     IYEYGLSDEDRKGLIDHLLTKIRNVSPPVMLSLRNAFRNKSRLAFTLITLTMAGAMFI
                     SAFSTRDSLTQQIGEIVRYVHFDAALSVPAGSNRDTILREALRIPGIAIAEGWENSSA
                     VVLHTDGSEGDEVDVQGVPVDSQTIDARFIAGRWLQAADTRQVVINDDFLEQEPAVTI
                     GTEIILKTGDRTRTFEVVGIVSKHLIGPRVYMSFNSFAKLTGRQDQIDSIRVRADPDR
                     VGSADEQDRMAALLEQHFKDAGLSSATATTQHTTFEMFTSAFDVILLILIIMAGLLAI
                     VGGLSLTGTMGMNILERTREIGVLRAVGASNEAVRRVVVVEGVVVGLMSGLLGTLLAV
                     PVGWLLAGAVIRAVLKANVSYQFSLLGVGLWLAVIILIGVFSSLGPARNAVNLSVREV
                     LDYE"
     gene            complement(3940..4686)
                     /locus_tag="VFF70_10130"
     CDS             complement(3940..4686)
                     /locus_tag="VFF70_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45094.1"
                     /translation="MNSATANSTASIGSLQPDSDAHFIELRSVVKKYQTPAGEFTALK
                     GINLQVARGEFVAVLGKSGAGKSTMINLMTGIDNPTSGEILIDRLPIHTLPEDQRAQW
                     RGRNLGMVFQFFQLLPSLTLIENITLSMDFLNTYPRRERKARALHLLEQVGIIEHAYK
                     IPSRISGGQQQRVAIARALANDPAILVADEPTGNLDSHTAHEIFDLFSSLLEHGKTLL
                     VVTHDKEIAARANRRIEMIDGEIVANGSVH"
     gene            4955..>5883
                     /locus_tag="VFF70_10135"
     CDS             4955..>5883
                     /locus_tag="VFF70_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HZY45095.1"
                     /translation="MLKVAVIGGGSTYTPELINGFLARVDTFPLRELWLMDIDPDRLA
                     IVGKFAQRMAEAKHAPFKIYLTTDRAAAIHGASYVTTQLRVGMMEARRRDEYLGQRHG
                     LVGQETTGIGGMAKALRTIPVILEIANEMRRSAPNALLVNFTNPAGLVTEALQRYAPD
                     IASVGVCNVALNTKLQFVDRLQKQLNSTIEPARTQLKTLGLNHLTWHYGFTLDGEDMW
                     SHVIQSFLEELQSDPEPEWDVRTIASLRMIPNYYLQYFYYTQHKLAEQKKWPPSRAEE
                     VMSIEDGLLRQYAEPDRTQPPDDLMKRGGAYYST"
BASE COUNT         1436 a         1696 c         1456 g         1295 t
ORIGIN      
        1 accaatgaca gattcgcctc agcggctttg atcgccgcct gcttctgtgc caagttcgct
       61 cgaaggtccg gcgcggccaa acgggcgatc agatcccccg ctttgacgac atcaccatct
      121 ttgaccaatg gatcgatcac cgtgcccgcc tgcccgaaac tcaacgtcgt ccacttcgcg
      181 ggcagtactg tgcccgtcgc gttcacgatc ggcgaaatgt tcttaacgga tgaagccacc
      241 gccgtggacg gaggcgtcgc cgtagatgga gcaccacacg ccgaaaggat cagactagcg
      301 agacaaatca gcccgataaa ataaaatttg attttttgaa acttcatacg aatttatcca
      361 gtgacaaaat gataggaact gttcggcctt ggccgccgcg tgtcattccc gcatgcatta
      421 agcgagaatc cagagtccct atcagcataa cttgccaaca gtcaatctga atgcccgata
      481 cacatgcggt aaagcacaca gtgcgtaccg gaagcatgcg ggcatgacgg ctgagcagtc
      541 acaaataatc gaaccgcatc cttcaccatt actcatcact catcactctt cactcattgt
      601 taattgttcc ggtattcatt cacgatctca ccatccgcga gtacaatcgt ccgctcagct
      661 tcattggcca gatcggtatc gtgcgtcacc atcaagatcg ttttgccgtc ggcgactaaa
      721 ttcttgaaca gctgcatcac cgattcagcc gtcttcgaat cgaggttgcc cgtcggttca
      781 tctgtcgcga cgatcggcgg atcgttcgcc agcgctcggg cgatcgccgc tctttgttgt
      841 tggccgcctg ccaagtgatt gggtaattgg taagcatgct cggcgatgcc gacgagttcc
      901 attaaataca tcgcacgttc gttgcgctct tgcggcgtgt agatgttgca gaagtccatc
      961 ggcagacgga cattctccag caccgtcaat gtcggcagca actgaaagaa ttgaaagacc
     1021 acgcccatgt gcgaaccgcg ccacgtagcg atttgacctt cattcaacgt atgcaccgcc
     1081 ataccaccaa tgtgtatttc gcccttcgtc ggtcgatcga tcccggtcag catgttgatc
     1141 aacgtcgtct tgccgctgcc cgacttgccg atgatcgata cgaattcgcc cgaaccgatc
     1201 gatagatcga tgcctttgag cgcctcgaat tttccggcgg gcgtctcgta agtcttgacg
     1261 atgttatgca gtttgatcaa cgtcccgtcg tcattgcgat tgttgttcgc gggtgtattg
     1321 tacttactct taacattagc catgattgct gaactcgcac atcgattcac cgcagagcac
     1381 gcttagaccg ccgagttttt tatatctctc agtgtattct acgatctctg cggtaaaagt
     1441 tatttactcg taatccagaa cttctcttac actcagattt acggcattgc gcgccggccc
     1501 caaactggaa aacacgccga tcaaaataat gacggccagc cagagtccga cgcccaacag
     1561 cgagaattga taactcacat tagccttcag cacggcgcgg ataaccgcac cggccagcag
     1621 ccagccgact ggcaccgcga gcagcgtacc cagcaggccg ctcatcagac caacgaccac
     1681 gccttcgacc acgaccacac gccgcactgc ctcattcgac gcgccgaccg cgcgcagcac
     1741 accgatctcg cgtgtgcgct ccaggatgtt catgcccatc gtaccagtca aactcaaacc
     1801 accgacaatc gccaacaaac cagccataat aatcaagatc aataagataa catcgaaggc
     1861 gctagtaaac atttcgaatg tagtgtgctg cgtagtagcc gtagccgagc taagacccgc
     1921 atctttgaaa tgctgctcca acagcgcggc cattcgatct tgctcgtcgg ccgagccaac
     1981 ccgatcggga tcagcgcgca cacggatgga atcgatctga tcctgccgcc cggtcaactt
     2041 tgcaaaagaa ttgaaactca tatacacccg cgggccgatc aaatgcttgg agacaatgcc
     2101 cacgacttca aacgtgcgcg tgcgatcgcc ggtcttcaaa ataatctcag tgccgatcgt
     2161 cacagccggt tcctgctcca agaaatcatc attgatgacc acttgacgcg tatcggcagc
     2221 ttgcagccaa cgtcccgcga taaaacgcgc gtcgatcgtt tgactatcca ccggcacacc
     2281 ttggacatcg acttcatcgc cttcgctgcc atccgtatgc aaaacaactg cactagaatt
     2341 ctcccagcct tcagcaatcg ctatgcccgg aatgcgcagc gcctcacgca gaatcgtgtc
     2401 gcgattcgat ccggcgggca cactcaacgc ggcgtcaaag tgaacgtagc ggacgatctc
     2461 gccaatctgc tgcgtcagtg aatcgcgcgt gctgaacgcc gagatgaaca tggcgcccgc
     2521 catcgtcaac gtgatcaacg taaaagccaa acgcgattta ttgcgaaacg cattgcgcag
     2581 cgacaacatc accggcgggc tgacgttgcg aatcttcgtc agtaaatgat cgatcaaccc
     2641 ttttcgatct tcgtcgctca gaccatactc atagatcgca tcatagaccg aaatgcgcgt
     2701 gccggaaata atcggatata acgctactcc gatcggcatc agcagcccga cgaagatctg
     2761 caccagcgca atattcggcg gcaaagtcac ttgagaaata tcgaagttca tgatgttgcc
     2821 ggccaatgag gccagtcccg acgcgccgat cacacccaac ggcagaccga tcaataaacc
     2881 gagcacgctc aagacaacta cgttgagcac atacatgccg atgatttgcc gccgcaccgc
     2941 gccgatcgat cgcatgatac cgatctgctt gatctgctgc gacatgatcg ccgaaatggt
     3001 attcaccact aaaccgccgg tcaagaaaat taccagaatg cccatcactt gcaagaccag
     3061 cagcatgccg ataatttgat tctgcgccca gtgctgaccc ggatcggcgc tgaagcccgg
     3121 tatctgaatg cgattgaccg tgtagcccga tcgctgaatg atgcgatcgc gcgcgagtcc
     3181 cgcgacagcc agatcgtgcg cgcgactaag tggatcgtct gcggtgatga tatcgagccg
     3241 attataagtc gtgcccaaac ccatccaggc cagcgtatcc atcgacacat aaccgctcgc
     3301 ttgatggcct aagttgaaag gccgcaagat cagtgaatgc acaatgccga caaccatcac
     3361 tggataacga cgctgattgt tcacctcgat catgatctga tcgccgacag ttacaccgag
     3421 tccaacggcc gattggcgct caagcaaaat ctcgcgcacg cccggcatag cagcacctgc
     3481 ttcaacatga tacttgtcaa tgcgcagatc actgaaatct gggacagcga tcaagttgat
     3541 gtcgtaggat tttccatcga cgcgatagac cgtttcagtc gccagccgct tcgcttgagc
     3601 gtcggcgact tccggcatgc cggcgacagc cgtggctaaa ctcttatcga acgtcgaaac
     3661 aaaaatttgg acgagcgcag gattgcctgc ggcgtatgat tcgaacagat cgcgctcgat
     3721 gatgcctttg gcattgttga tcatgccgac agcgccgaca cccacggcga tcgataagat
     3781 caccaagatc gatcgggatt tgtgatccca caggtctttg agaattttga tccagcgggt
     3841 gcttagttcc actgtttttc cccagagtaa cgttactcgt gccggagcgc cagctctggc
     3901 agacatcggc gaagctgacg cttcgccttg agcctttaat taatgtaccg atccattggc
     3961 gacgatctcg ccatcgatca tctcgatccg gcgattggcc cgcgccgcaa tttctttatc
     4021 gtgcgtcaca accaataatg ttttgccgtg ctccagcaaa cttgaaaaca gatcgaagat
     4081 ttcgtgtgcg gtgtgcgaat cgaggttgcc ggtgggttca tcggccacca atatcgctgg
     4141 atcgttcgcc aaggcgcgcg caatcgccac acgctgctgc tgtccgccag agatgcgtga
     4201 cgggattttg taagcatgct caataatccc aacttgttct aagagatgca gggcgcgcgc
     4261 tttgcgctct cggcgtggat aagtgttgag gaagtccatc gagagggtga tgttctcaat
     4321 caaggtcagc gatggcaaca attgaaagaa ctgaaacacc atgcccagat tgcggccgcg
     4381 ccactgcgcg cgttgatctt ctggcagcgt atgaatcggc agtcgatcga tcaagatttc
     4441 gcccgacgtc ggattgtcga tgccagtcat caaattgatc atcgtcgact tgcctgcgcc
     4501 ggatttgccc agcacggcga caaattcgcc gcgcgcaacc tgcaggttga tgcctttcaa
     4561 ggccgtaaac tcgcccgcgg gtgtctgata tttttttacg acgctgcgca gttcaatgaa
     4621 gtgggcatca ctatcgggtt gcagtgaacc gatcgaggcc gtgctattgg ctgtggctga
     4681 attcatgtgg actttccaag acaaagccgc ctcaatgagt tgggcggctc gttaattcaa
     4741 caaaacttgc tgacaaacgc gcaagacaat agtaacaatg aaattgatat ccgtcaatga
     4801 tctgcgggcg aagccagcga agcgtccttc gcgactcgat caatatgccg cggccgatca
     4861 gctgccgctt ttcatagcgc ctcataaatc atgtatacta tcatccagca tcgcggtttg
     4921 gtcatcacta gcgttgaaaa atagaaagga catcatgctg aaagttgcag tcatcggggg
     4981 cggatcaacc tatacgcccg aattgatcaa cggctttctc gcgcgcgtcg atacttttcc
     5041 gctgcgcgaa ttgtggctga tggacatcga tccggatcga ttagcgatcg tcggcaagtt
     5101 tgcgcagcgc atggcggaag cgaagcacgc gccctttaag atttatctga ccaccgatcg
     5161 agcggcggcc atccacggtg catcgtatgt gacaactcaa ttaagagtag gcatgatgga
     5221 agctcggcgt cgcgatgaat acctcggtca gcggcacggg ctggtcggtc aagagaccac
     5281 cggcatcggc ggcatggcga aagcgctgcg caccattcca gtcattcttg aaatcgcaaa
     5341 cgagatgcga cgatcagcac ccaacgcctt attggtgaac ttcactaatc cggctgggtt
     5401 ggtgaccgaa gccctgcaac gttacgcgcc cgatatcgct tcggtcggcg tgtgcaatgt
     5461 ggcgctgaac actaaattgc aattcgtcga tcgattgcag aaacaactga acagcaccat
     5521 cgagccagcg cgcacacaat tgaaaacgct cggcctcaat catctcactt ggcattacgg
     5581 cttcacgctc gatggtgaag atatgtggtc gcatgtcatt cagagttttc tggaagaact
     5641 ccaatccgat ccagaacccg aatgggatgt ccgcacgatc gcatcattgc gcatgatccc
     5701 gaattattat ttacaatatt tctattacac tcagcacaag ttggccgaac aaaaaaagtg
     5761 gccgccctcg cgcgccgaag aagtcatgtc gatcgaagac ggtttgctgc ggcagtatgc
     5821 tgaacccgat cgaacgcagc cgcccgacga tttgatgaaa cgcggaggcg catattactc
     5881 aac
//
LOCUS       DASRTJ010000500         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_334800,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000500 DASRTJ010000000
VERSION     DASRTJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334800"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFF70_10140"
     CDS             complement(<1..288)
                     /locus_tag="VFF70_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZY45096.1"
                     /translation="MVLEILRGLSITLREMFKPPVTIQYPEIKRPVRQRFRGRHELRR
                     YADGLERCIGCALCAAACPADAIYVEAAENTDEKRFSPGERYAATYEINMLR"
     gene            complement(406..1449)
                     /gene="nuoH"
                     /locus_tag="VFF70_10145"
     CDS             complement(406..1449)
                     /gene="nuoH"
                     /locus_tag="VFF70_10145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZY45097.1"
                     /translation="MTLGTSLLIWVIHSVLLAVILLTGFAYTTLLERKVLARIQSRIG
                     PNRAGPFGIFQPLADGVKLIFKEELTPGGADRLTFLIAPIMAAVPAVVVFAVIPLGGT
                     ANFFGTPIELTLAPHLNVGILYVLAITSISVYGIVLAGWASNNKYSMMGGLRSSAQMI
                     SYELAMGLSIVALVLVAGTLDMNSIVDQQKQVWFVFLQPVAALVFMISALAEVNRSPF
                     DLPEAEQELTAGYVTEYSGMKFALFFMAEYIKMAVIGGLAATFFFGGYREPLPLFIVK
                     AMPLLSVDNTPWLGPIYFSMKVIFFLFIQIWVRATLPRFRYDKLMKFGWKFLLPVSLV
                     WVMVTAFVILIRG"
     gene            complement(1446..>3536)
                     /locus_tag="VFF70_10150"
     CDS             complement(1446..>3536)
                     /locus_tag="VFF70_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY45098.1"
                     /translation="MQVRTQNETVKKAQKDVLEFLLTSHPLDCPICDKGGECPLQNLT
                     LGFGPGVTRFDFNEKQHLAKHFPLGDLVFLDKERCIQCARCIRFQDEIAEDHVLGFYE
                     RGRTMQIQTTSEPGFDSKLSGNTTDICPVGALTTSDFRFRARPWEMTNVPSICTHCPV
                     GCNITLGTRRSSKGGGDWDILRVMPRQNEEVNEIWICDKGRFGHHFTRSADRLTTPLI
                     KKNGKFVEATWDEALNLVADRLKLADGHVAGFIGDRVSNEDAYLFGKLFRDVLKSNRI
                     SNRPHVAGIDLVQRYGVGKGTNLKDLGRGDVIVVVAGDVEESAPIYSLRLMAAAKRGA
                     TLIVANSRETKIDRYTKHSLRYKAGTEAQLVYGLLSAVSNQQSAISDHLSNYNASKVA
                     SITGVDETAIDEAGKAIGAAKNLIVVFGDEQPYPAMEHGQYTNEGRALAQACANLVVT
                     TKHVGTANNGLLPLWPHNNTQGVFEMLSATGASWSNADNLKAIYIVGTDPIGEGQHLP
                     KSEFVIVQELFMTKTAHQADVILPAASFAERDGTFTNAERRVQRYYKALPPLGEGHPD
                     WDILQAVANRSGVKWGCYTSEAVMDEIAATIPAYSGMIYPALAVMKEEWPPVGGDDLY
                     FGGTAYDNRGGVGLQLPSLVELDNRSELNWIEPGTRSDGQIIPRKLNRHGTLIDQSKV
                     YEPRLIEPMVEYQA"
BASE COUNT          812 a         1088 c          875 g          761 t
ORIGIN      
        1 gcgcagcata ttgatctcat acgtcgcggc atagcgctca cccggcgaga aacgtttctc
       61 gtcggtattc tcggccgctt cgacgtagat cgcatcggcg ggacaagccg ccgcgcacaa
      121 cgcacaaccg atgcagcgct ccagtccatc ggcatagcgg cgcaattcat ggcggccacg
      181 gaaacgctgt cgaacaggcc gcttgatctc aggatattga atggtgaccg gcggtttgaa
      241 catttctctt agcgtgatgc tcaagcctcg caaaatttct agaaccatat taaactccat
      301 caaattctat tgtcattccg agcgaagcga ggaatctcta ccactatcag ccgaggttat
      361 tccccgcctc gcggctcaga atgacacaat catttcgtgg ttctttcagc ctctaatcaa
      421 aatcacgaac gccgtcacca tcacccacac cagcgatacc ggcagcagga acttccaacc
      481 gaacttcatc agcttgtcgt agcgaaaacg aggcagcgtc gcgcgcaccc aaatctggat
      541 gaacaagaag aagatcacct tcatcgagaa atagatcggg ccaagccacg gcgtgttatc
      601 gacgctcaac agcggcatcg ccttcacgat gaacaacggc agcggctctc gatacccgcc
      661 gaagaagaac gtcgcggcca gtccgccgat gaccgccatc ttgatatatt cggccatgaa
      721 gaacagcgca aatttcatgc cgctgtattc ggtcacgtaa cccgccgtca attcttgttc
      781 cgcttccggc agatcgaacg gcgagcgatt gacttccgcc agcgccgaga tcatgaacac
      841 caacgccgcg accggctgta agaacacgaa ccagacttgc ttctgctgat cgacgatcga
      901 gttcatatcg agcgtgcccg ccaccagcac cagcgcgacg atcgacaagc ccatcgccaa
      961 ttcgtacgaa atcatctgcg ccgacgatcg cagcccgccc atcatcgaat acttattgtt
     1021 gctcgcccag cctgccagca caatgccata cacgctgatc gatgtgatcg ccagcacata
     1081 taaaatgccg acgttcaaat gaggggccaa cgttagctcg atcggcgtgc cgaagaaatt
     1141 ggcggtgccg ccgagtggga tcacggcgaa cacgacgaca gccggcactg cggccatgat
     1201 cggcgcaatc aagaacgtca atcgatcggc gccccctggc gtcaattcct ctttgaagat
     1261 caacttgacg ccgtcggcca gtggctggaa tatgccgaac ggcccggcgc ggttcggtcc
     1321 gatgcgcgat tgaatgcgcg ccaacacctt gcgctccaac aacgtggtgt acgcaaagcc
     1381 cgtcagcaaa ataaccgcca acagcaccga atgaatgacc cagatcaaaa ggctcgtgcc
     1441 gagagtcatg cttgatactc caccataggc tcgatcaatc gcggctcata cactttggat
     1501 tgatcgatca atgtgccgtg tcgattcaac ttgcgcggga taatctgccc atccgatcga
     1561 gttcccggct cgatccaatt taattccgat cggttatcca gctcgacgag cgagggtaat
     1621 tgtaaaccca caccgccgcg attgtcatat gctgtgccgc cgaaatacaa atcatcgcca
     1681 ccaacgggcg gccattcttc tttcatcact gccaaggccg gataaatcat tccgctataa
     1741 gcgggaatcg tcgcagcgat ctcatccatc accgcttccg aagtgtagca gccccacttc
     1801 acgcccgatc gattggcgac cgcttgaagg atgtcccaat cgggatggcc ttcgcccagc
     1861 ggcggcagcg ctttgtaata gcgctgcacg cgccgctcgg catttgtaaa agttccatcg
     1921 cgttcagcaa atgaagcggc gggcaggatc acatccgctt gatgtgctgt ctttgtcatg
     1981 aacaactctt gcacgatcac aaattcactc ttcggcagat gctgtccctc gccgatcgga
     2041 tcggtgccga cgatatagat cgctttcaag ttatccgcgt tcgaccaact cgcgcccgtc
     2101 gctgacaaca tctcgaacac gccttgcgta ttgttgtgcg gccacaaggg taacaagccg
     2161 ttgtttgctg tgccgacatg cttcgtcgtc accaccagat tcgcgcaggc ttgagcgagc
     2221 gcgcggcctt cattagtata ttggccgtgc tccatcgccg gatacggctg ctcgtcgccg
     2281 aacaccacga tcaagttctt cgccgccccg atcgctttac ccgcctcatc gatcgctgtt
     2341 tcatcaacac cagtaattga tgcaacttta ctcgcgttgt aattgcttaa gtgatcgctg
     2401 atggctgact gctgattgct gaccgctgat agcaaaccgt acaccaactg cgcttcagtt
     2461 ccagctttgt agcgtagtga atgcttcgta tatcgatcta tttttgtttc gcgcgaattc
     2521 gccacaatca acgtcgcgcc gcgcttggcc gctgccatca agcgcaggga atagatcggt
     2581 gcgctctctt ccacgtcacc ggcgacgacc acaatcacat cgccgcgacc gaggtctttg
     2641 agattcgtgc ctttgcccac accataacgc tgcaccagat cgatgcccgc gacatgcggc
     2701 cgattagaaa ttcgattcga cttcaacaca tcgcggaaca acttgccaaa taaatatgca
     2761 tcttcattcg acacacgatc gccgatgaat ccagccacat gcccgtcggc cagcttcaac
     2821 cgatcggcga ccagattcaa cgcttcgtcc cacgtcgctt cgacaaactt gccgttcttc
     2881 ttgatcagcg gcgtcgtcaa tcgatcggca ctgcgcgtga agtgatgacc gaagcggcct
     2941 ttgtcgcaaa tccagatttc attgacttct tcgttttggc gcggcatcac acgcaagatg
     3001 tcccaatcgc cgccgccctt cgatgatcga cgcgtgccta gcgtgatgtt gcaacccaca
     3061 ggacagtgcg tgcagatcga tggcacgttg gtcatttccc acggccgcgc gcggaaacga
     3121 aaatcgctgg tggtcaacgc gccgacgggg cagatgtccg ttgtgttgcc ggataacttc
     3181 gaatcgaagc ccggctctga ggtcgtttga atttgcatcg tgcggccgcg ctcataaaaa
     3241 ccgagtacgt gatcctcggc gatttcatcc tggaagcgaa tgcagcgcgc gcactgaatg
     3301 cagcgctctt tatcgagaaa aaccaaatcg cccagcggaa aatgtttggc gagatgctgc
     3361 ttctcattga agtcgaagcg cgtcacgccc ggcccaaagc ccagcgtcag attttgcagc
     3421 gggcactcgc cgcccttatc gcaaatcggg caatcgagcg ggtgcgatgt cagcagaaac
     3481 tccagcacgt ccttctgtgc cttcttcacc gtctcgtttt gcgtgcggac ctgcat
//
LOCUS       DASRTJ010000501         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_165053,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000501 DASRTJ010000000
VERSION     DASRTJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165053"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..817
                     /locus_tag="VFF70_10155"
     CDS             140..817
                     /locus_tag="VFF70_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45099.1"
                     /translation="MTKIRVLLADDQDIIRTGLTIILNHQPDIEVVGQAADGAQAIDL
                     VKQLNPDVVLMDIKMPNINGIQATRQIVAARPQTQIIILTTYDTDDWVFDGIRAGAIG
                     YLLKDTPSQDLAAAVRGAMRGESQIDPAVARKVLREFQHVTRDRAPAPKQTFDEPELE
                     RLTDREEDVLKLLASGLSNKDIASQLSLSEGTVKNHISAILAKLHANDRTQAVLTALK
                     RGLVDLE"
     gene            complement(860..2152)
                     /locus_tag="VFF70_10160"
     CDS             complement(860..2152)
                     /locus_tag="VFF70_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY45100.1"
                     /translation="MKLTTHHIPKLVLIDHEFQVPLDHTKPNGDHLSVFAREVATTDR
                     ENDKLPWLVFLQGGPGIQSPRLDNNHEQWWTRATKEYRVLLLDQRGTGRSTPVNFQSL
                     ARLARPQAQADYLKLFRADSIVRDCELIRTELIGTEKWSTLGQSYGGFCTATYLSIAP
                     EGLRESIITGGLPPIRQTPDDVYRATYKRVLGKNQKYYARYPDDIARARDIADFLMKN
                     DVRFPNGDRFTVGRFQDLGKNFGASNGLEFVHYLLEIAFIEGAHGREINLAFLRGVEN
                     QSIYDTNPIYVMLQEACYTQHAASNWSAHRLLAEYPQFDVTKNDVVQFTGEMMYPKEF
                     DDYQALRPLKEAANILAEYADWPNLYDVKVLQANEVPVAATIYWDDMYVERAYAEEAA
                     SLIRGCKTWVTNEYEHNALRADGERVLDRLLGMLHGNS"
     gene            complement(2164..>2917)
                     /locus_tag="VFF70_10165"
     CDS             complement(2164..>2917)
                     /locus_tag="VFF70_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45101.1"
                     /translation="LVLQFGLSGMQLIYGILTAISAIVFLVVVREKPPTPASPAAELE
                     RALMLDGLRQILRIREFWYLAIVLFVGNGIFNGLSTWVETIVRPRGLSISEAGDLGGA
                     MLLGGVIGSIVMPALSDRLRKRKALMVVGFAIAIPGMIGAAFGASYFLLLASFFWLGL
                     FTTGIAPVAYQYGAEVTYPVPEGTSNGLFAMAGQISVVFIFAMGWSNDVLKSFAPSLI
                     TLIVLMLIGVVLLALLKESPMMKVQTAIDSNS"
BASE COUNT          680 a          782 c          807 g          648 t
ORIGIN      
        1 agtcagcgac gaacgctttg gtttaattgg catgcgtgaa cgggccgaaa tgatcggcgg
       61 tatgttcagc gtcgaaagtc atatcgggca gggtacaaaa gtatctgtat ctttgccgct
      121 gcgatcgatc gagtagatga tgaccaagat tcgtgtgttg ctggctgacg atcaagatat
      181 tatccgcact ggattgacga tcattctgaa tcatcaaccg gatattgaag ttgtcggtca
      241 ggcggcggac ggcgcgcagg cgatcgatct ggtgaagcag ctgaaccccg atgtggtctt
      301 gatggacatc aagatgccga acatcaacgg cattcaggcc acgcgccaaa ttgtggcggc
      361 tcggccgcag acgcagatca ttatcctgac gacgtacgac acggatgatt gggtgttcga
      421 cggcattcgc gccggagcga ttggttactt attgaaggat acgccgtcgc aggatttagc
      481 ggcggcggtg cgcggcgcaa tgcgcggcga atcgcagatc gatccggcgg tggcacgcaa
      541 agtgctgcgc gagtttcaac atgtgacgcg cgatcgagcc cctgcgccga aacagacatt
      601 cgacgaaccg gagttggagc gcctgaccga tcgggaagaa gatgtactca agctgctggc
      661 ttcgggctta tccaacaagg acatcgcgag tcaactctct ttgagtgaag gcaccgttaa
      721 aaatcacatc agcgcgattc tggctaagtt acatgccaac gatcgaacgc aggcagtgtt
      781 gacggcgttg aagcgaggac tggttgattt ggagtagtcg atagactcga agggttttca
      841 aaacccttcg agtttttatt tacgaattac cgtgcagcat ccccaacaat cgatcgagca
      901 cacgttcgcc gtcggcgcgc agggcgttgt gctcatattc attcgtcacc cacgttttgc
      961 agccacgaat caaactcgcc gcttcttcgg catacgcccg ctcgacgtac atatcatccc
     1021 aatagattgt ggccgccacc ggcacttcat tcgcttgcaa aactttcaca tcatatagat
     1081 tcggccaatc ggcatactcg gccaaaatgt tcgcggcctc tttcaatggt cgcagcgctt
     1141 gatagtcgtc aaactccttc ggatacatca tctcgcccgt aaactgcact acatcattct
     1201 tggtcacatc aaactgcgga tactctgcca gcaatcgatg tgccgaccag ttcgatgcgg
     1261 cgtgttgcgt gtagcacgct tcttgcagca tcacgtagat cggattggtg tcgtagatcg
     1321 attggttttc gacgccccgc aggaaagcga gattgatctc gcggccatgc gcaccctcga
     1381 tgaacgcgat ctccaacaag taatgtacga attccaaacc gttgctcgcg ccgaaatttt
     1441 tgccgaggtc ttggaagcga ccaacggtga atcgatcgcc attggggaac cgaacatcgt
     1501 tcttcattaa gaagtccgcg atatcgcggg cgcgcgcgat gtcatcggga taacgcgcat
     1561 aatatttttg attcttgccc agcacgcgct tgtaagttgc gcgatacaca tcgtcgggcg
     1621 tttgcctgat cggcggcagg ccaccggtga tgatcgattc gcgcaagcct tcgggcgcga
     1681 tcgataagta agtggcagtg cagaatccgc cgtaactttg gccgagcgtg ctccactttt
     1741 ccgtgccgat caattctgtg cggatcaatt cgcaatctcg cacgatcgag tcggcgcgaa
     1801 aaagtttcag gtaatcggct tgcgcttgcg gtctagccaa gcgcgccagc gattgaaaat
     1861 tgacgggcgt gctgcggccc gtaccacgct gatcgagcag cagaacgcga tactctttcg
     1921 tcgcacgcgt ccaccattgc tcgtgattgt tatcgaggcg cggcgattga atgcccggcc
     1981 cgccttgcag aaacacgagc catggcagtt tgtcattctc tcgatcggtt gtagctactt
     2041 cgcgcgcgaa tacggaaagg tgatcaccgt tgggtttggt gtgatcgagt gggacttgga
     2101 attcgtgatc gatcagaact aactttggga tgtgatgagt tgtgagtttc atgaagagct
     2161 ttcttatgaa ttcgagtcta ttgcggtttg cactttcatc atcggcgatt ccttgagcag
     2221 cgccagcaac acgacgccga tcaacatcaa gacgatcagt gtgatcaacg atggcgcgaa
     2281 tgatttcagc acatcgttcg accagcccat cgcaaagatg aacacgaccg agatttgtcc
     2341 agccatggcg aacaatccgt tcgaagtgcc ttcaggaaca ggataagtta cctctgcgcc
     2401 gtattgatag gcgacgggcg cgatgccggt ggtgaataag ccgagccaga aaaatgatgc
     2461 gagtaacaag aagtaacttg caccgaaggc cgcgccgatc atgccgggaa tggcgatcgc
     2521 gaagccgacg accatcaacg cttttcgctt gcgcagccga tcggatagcg cgggcatcac
     2581 gatcgatccg atgacgccgc ccagcagcat cgcgccgccc agatcgcccg cctcgctgat
     2641 cgataatccg cgtggccgca cgatcgtttc gacccacgtg cttaagccat tgaagatgcc
     2701 attgccgaca aataacacga tcgccaaata ccagaattcg cggatgcgca aaatttgccg
     2761 caggccgtcg agcatcagcg cgcgttcgag ttctgccgcc ggactggcgg gcgtcggcgg
     2821 tttttcgcgc acgacgacca agaacacgat cgcggagatg gccgtcagaa tgccataaat
     2881 caactgcatg ccactcaagc cgaattgcaa aacgagc
//
LOCUS       DASRTJ010000502         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_354251,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000502 DASRTJ010000000
VERSION     DASRTJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354251"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFF70_10170"
     CDS             complement(<1..489)
                     /locus_tag="VFF70_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45102.1"
                     /translation="MTNDPQLPQPVESKQPYRAPIATALLLIGIMLAGAYFRFVGIDW
                     DEEQHLHPDERFLTMVEAAIQLPKNVGQYFDSLTSPLNPYNNNFGLFVYGDLPIVFTR
                     YVAAALDGLCYNTDAHACLDQHSATFSFSDYGHVYLLGRILSALFDLGTLLWLFLIAR
                     KLY"
     gene            complement(575..3109)
                     /locus_tag="VFF70_10175"
     CDS             complement(575..3109)
                     /locus_tag="VFF70_10175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45103.1"
                     /translation="MQIGIELTLALGLMGLGHYYVSTQRDQYPLDGYVFYALALGVFA
                     HVWHITRADQDPIWSALRGAFRTGWTLLRDSLSLIWRGLRELLPMMSLRTRFAIVAVF
                     NILAAILAIVLPQAISLWLIVWLGSIFVLIGPLLSRSITRAAPQRSETWIEPLKSIGR
                     APKLVGLLIAIGLLIAGQIVVSANDPASRSPAVSWIQDELKLGLRSPATALGGLGLLL
                     IGLIVFAIVTRRSAVIDAPRLSVEVPTPAGKRVSNRWIAVAAIGYAIWLSMIRSIAED
                     ASGWAGVLPWLISIGLIAVCWWKIDRSRGVRLAIRLDRSEVIGLAMAAVAILIVFVYR
                     INDVPNSMWGDEGAFFSTARDIAQGKATLDFFGLGAYGTFPTPGSVFQSIFLSIFGAN
                     IIAWRLGSAVAAWLAIFPLYYLTRATLGKRVAWISIALYAISPYVLTYARMGYNNSQS
                     IAPVAVTLMLTWFAIKRSSLFFAFLAGCVGGLGFMTYTAAELGIVLAVGWLIWMWIMR
                     SGRRRTIALAIAAYLLGSAMIAAPPIVYQAARLPQMFAYKQVESIFANVIYARDLYPD
                     DQLFASASVIKVGDQDLFYDPSIYSQLLARGVVRTALGFHISSVITENYLIGGLAEPF
                     GAIYLLGLGWCLIRVRRPGYAIWSAWLIVGAITLSVIDTYPPRAAHMLPVVASIAVLS
                     SLGLVAGIDTLAHVIGGIPDRIKSIILIGAVSVLAFFGLRTYFIEMPAHFPPDLENTM
                     FWQAQSMPRGSDITLIQPEGLPDEFVPWGLRELDLGVNFHLIKPADIATADLKSLCPN
                     VCRFYFLQADFYAASNRLTQIFGQKSKQPYPDVIGPNGFYEFNPNE"
BASE COUNT          905 a          902 c          817 g          612 t
ORIGIN      
        1 ataaagtttg cgcgcaatca agaacagcca cagcagcgtt cctagatcga atagtgcgga
       61 caagatgcgg ccaagcaaat agacgtgccc ataatcgctg aaactaaagg tcgcactgtg
      121 ttgatcgaga caagcgtgcg cgtccgtgtt gtaacacaac ccgtcgaggg cagcggccac
      181 atagcgcgtg aacacgatcg gcagatcgcc atagacaaac aaaccaaagt tgttgttgta
      241 agggttcagc ggtgaagtaa gcgaatcgaa atactggccg acgttcttcg gtaattgaat
      301 cgcggcttcg accatggtga gaaaacgttc gtcgggatgc agatgctgtt cttcgtccca
      361 atcgatgccg acgaagcgga agtacgcgcc cgccagcata ataccgatca gcaaaagcgc
      421 cgttgcgatc ggcgcgcgat acggctgctt ggattcaacc ggttgaggaa gttgtggatc
      481 gtttgtcatt tagtcagata gttcgatagt cgagtagttt cagctaacgc tggctgtatt
      541 cccgcttgag caggaatgcc tgtgcgcaac attatcattc gttcggattg aattcataaa
      601 aaccattggg gccaatcaca tctggataag gctgctttga tttctggccg aaaatttggg
      661 tcaagcgatt ggatgccgca tagaagtcag cctgaaggaa atagaaacgg catacattcg
      721 ggcataaact ttttagatct gcagtagcga tgtcagcggg tttgatcaaa tgaaagttca
      781 cgcccagatc gagttcacgc agcccccacg gcacaaactc gtcgggcaga ccttccggct
      841 ggatcaacgt gatgtccgat ccgcgcggca ttgattgagc ctgccaaaac attgtgttct
      901 ccaaatcggg cggaaagtga gcaggcattt cgataaaata tgtgcgcagc ccaaaaaacg
      961 ccaacacact cactgcgccg atcagaatga tcgatttaat ccgatccggt atcccgccga
     1021 tgacatgtgc cagagtatcg atgcctgcaa ccaaacccaa ggagctcaat accgcgatcg
     1081 acgcaacgac cggcagcata tgagccgcgc gcggcggata agtgtcgatg acgctcaaag
     1141 tgatcgcgcc gacaatcaac caggcggacc agattgcata gcctggacgc cgcacacgga
     1201 ttaagcacca gcccaagccc agcaaataga tcgcaccaaa cggttccgcc aagccaccga
     1261 tgaggtaatt ctcggtaata acactcgata tatgaaagcc taaggccgtg cggacaacgc
     1321 cacgcgctaa aagctgagaa taaatcgacg ggtcatagaa taaatcctga tcgccaactt
     1381 taatcacact tgccgaagcg aacaattgat catcaggata gagatcacgc gcgtagatca
     1441 cattagcgaa gattgattca acttgtttat aagcgaacat ctgcggcagc cgcgctgctt
     1501 gataaactat cggcggggcc gcgatcatcg cactccccaa cagataggcc gcaatcgcca
     1561 gcgcgatcgt acgacgccgc ccgctgcgca taatccacat ccagatcaac caaccgacag
     1621 ccagaacgat gcccaattca gcggcggtgt aagtcataaa acccagtccg cccacgcagc
     1681 ctgccaggaa tgcaaaaaat aaactactgc gtttgatcgc gaaccaggtc aacattaatg
     1741 tcacggccac gggcgcgatc gattggctgt tattgtatcc catccgtgca taggtcagca
     1801 cgtatggcga gatcgcatac aacgcgatcg aaatccaggc tacgcgtttg cccagtgtgg
     1861 cgcgggttaa ataatacagc ggaaagatcg ccagccatgc agcaacggct gagcctaagc
     1921 gccaggcgat aatattcgca ccgaagatcg agagaaaaat actctgaaag actgaacccg
     1981 gtgttgggaa agtcccatac gcacccaatc cgaaaaaatc caaagtcgct ttgccttggg
     2041 caatatcgcg cgcggtggaa aagaatgcgc cctcatcgcc ccacatacta ttcggcacat
     2101 cgttgatgcg atagacaaac acgattaaga tcgcgaccgc agccatcgct aagccaatca
     2161 cttcagatcg atcgagccgg atcgccaatc tcactccgcg cgatcgatcg atcttccacc
     2221 aacatacggc gatcagcccg atcgagataa gccacggcag cacgccggcc cagccgctgg
     2281 catcctcggc gattgatcgg atcatactca accagatcgc atacccgatc gctgcgacgg
     2341 caatccaacg gttgctcacg cgtttgccgg caggcgtcgg cacctccaca ctcaagcgcg
     2401 gagcatcgat caccgcactg cgccgcgtca cgattgcaaa cacgatcagg ccgatcaaca
     2461 acaaccctaa gccgcctaaa gcggtcgcgg gactgcgaag acccaatttt aattcgtcct
     2521 ggatccaact aacggccggt gaacgagaag caggatcatt tgccgaaaca acgatctgcc
     2581 cggcgatcag taacccgatc gcgatcaaca gtccaaccaa tttcggtgcg cgtccgatcg
     2641 atttcagtgg ctcaatccac gtttccgatc gctgtggagc agctcgggtg atcgatcgag
     2701 ataacaaagg accgatcagc acaaagatcg aaccgagcca cacgatcagc cacagcgaaa
     2761 tggcttgcgg cagaacaata gccaagattg cagctagtat attgaacacc gccacgatcg
     2821 caaagcgagt tcgcaacgac atcatcggca gcaattcacg cagaccgcgc caaatcaaac
     2881 tgagtgaatc gcgcagcaaa gtccaaccgg tgcgaaaagc gccgcgcaaa gcgctccaaa
     2941 ttggatcttg atcagcgcgc gtgatgtgcc acacatgggc aaacacgcct agtgcgagcg
     3001 cataaaaaac gtagccatcg agcggatact gatcgcgctg agtcgagacg tagtaatgcc
     3061 ctaagcccat gaggccaaga gccagggtta actcaattcc gatctgcatc cgcttgaatt
     3121 tcatatcgtt gggtaggacc gagtggcggc tcgccctgag ttatacgcaa caaagaaaac
     3181 gtacgcacca tttttcggca aaggctcgct gtagtaacgc cgcacatatt tgtcgg
//
LOCUS       DASRTJ010000503         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_334868,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000503 DASRTJ010000000
VERSION     DASRTJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334868"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFF70_10180"
     CDS             complement(<1..284)
                     /locus_tag="VFF70_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45104.1"
                     /translation="MKRNFVVSLFGSYIGFGMVFILLSAAGIVGARSSEVTRANVMHV
                     NAVAATPLTSTFTYQGQIKNSGTVVNGACQMAFRLYDAPAGSGLIGSPITT"
     gene            complement(532..1962)
                     /locus_tag="VFF70_10185"
     CDS             complement(532..1962)
                     /locus_tag="VFF70_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZY45105.1"
                     /translation="MASKNLQPGHPQLLTELRAAIGAEAVATDPATLTAYAYDGTWAE
                     QRPAVVLHPLSTQQVSSILRIADRERIPIVPRGAGTGLAGGSIPIEGSWCLNLARMNR
                     LLSVSAADSIVVTQPGVITASLQKEVEKQNLFYPPDPASLHMCTIGGNVSTNAGGPRC
                     LKYGVTADYVLGLEVVLPGGKIMRTGGQTIKNVSSYNITQLLVGSEGTLGVITEITLR
                     LIPKPIAQATALASFDKLSDACDAVGKMLSSGITPLVTEIMDHDVIRAVDQFQRDKRS
                     DLRLPIEAEALLLVAVDGDRDQVERDIDQVTSILKRAGSSDVRRANSPEEAEAMWEAR
                     RSVSPAVIRLGNARFGEDIVVPRSKIPQMVDIVKQIGKQQELLIVVYGHIGDGNLHPN
                     IICDRRDAEMMQRVERAAHAIFDAALELGGTISGEHGIGLLKTNYVSKAIDPVALSML
                     QSIKKLFDPHNILNPGKKLPGSTQVW"
     gene            complement(1916..>2204)
                     /locus_tag="VFF70_10190"
     CDS             complement(1916..>2204)
                     /locus_tag="VFF70_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45106.1"
                     /translation="KEVMTPSGRGKVIEVLPLRDSVLVQVAEMRIEVKRADLQPLAEL
                     EAFQKKIDQPCSGQHGCSCGASKRTGKNRDNKDQRDRGEQKPPTGPSTTSN"
BASE COUNT          475 a          627 c          597 g          505 t
ORIGIN      
        1 gtggtgattg gactgccgat caaaccactg cctgctggag cgtcatacaa ccgaaaggcc
       61 atctgacacg ctccattcac cacagtgccg ctgtttttta tttgaccttg ataggtaaag
      121 gtcgaggtga gcggcgtggc agcgacggcg ttaacgtgca taacattcgc tcgagtcacc
      181 tccgaactcc gcgcgccgac gatgcctgcc gcgctgagca ggatgaacac cattccaaat
      241 ccaatgtaac taccgaataa actaacaaca aagttgcgtt tcatgtctgc ctcccttggc
      301 aatgaacgtg gtctcagcgg tcggccctcg agaggacacg ctgagcaaca tttgattgaa
      361 gggctgcaca ctgagattag cctgcgaggc gcgatatgat caagctaatg tgtgctgtgg
      421 aaatgccgaa agttgagcac gatatcgcgc aatggtaaat tggcaaactt ccgcgccatg
      481 tcgattaaac aagactacgg gccgatcgga gaggcgaagt gacctcgccc tttaccacac
      541 ttgtgtgctg cccggcaatt tcttgccggg attcaggatg ttgtgtggat cgaacagttt
      601 cttaatggat tgcagcatgc tcaacgccac cggatcgatc gctttagaca cgtaatttgt
      661 tttcaacaag ccgatgccgt gttcgcccga aatcgttccg cccaattcaa gcgcggcgtc
      721 gaagatggcg tgtgccgctc gctcaacgcg ctgcatcatc tcggcgtcgc gtcgatcgca
      781 aatgatattc ggatgcaagt tgccatcgcc gatatgtcca tagacgacga tcaataattc
      841 ctgctgcttg ccaatttgtt tgacaatatc gaccatctgc ggaattttgc tgcgcggcac
      901 gacaatgtcc tcgccgaaac gcgcgttgcc cagtcgaatg accgccgggg acaccgatcg
      961 acgcgcttcc cacatcgctt cggcttcttc gggcgagttg gcccgtcgaa catccgatga
     1021 tccggctcgc ttcaagatcg aagtgacttg atcaatatcg cgttcgactt gatcgcgatc
     1081 accgtcgacg gcgaccagca gcagagcctc ggcctcgatc ggcaaacgca ggtccgatcg
     1141 tttgtcgcgt tgaaattgat cgaccgcgcg aatcacatca tgatccatga tctcggtgac
     1201 caagggtgtg atgccgctcg ataacatttt tccgacagcg tcacacgcgt cagaaagttt
     1261 atcgaaagac gctaaggccg tcgcttgcgc gatcggtttg gggatcaaac gcagcgttat
     1321 ttcagtaatg acgcccaacg tgccttccga tccaaccagc aactgagtga tgttgtagct
     1381 cgaaacattt ttgatcgtct gcccgccggt tcgcatgatc ttgccgccgg gcaatacgac
     1441 ttcgagaccg aggacgtaat cggcagtgac gccatacttc aaacagcgcg ggccgcccgc
     1501 attcgtgctg acgttgccgc cgatcgtgca catgtgcagt gaggccggat caggcggata
     1561 aaacaaattt tgtttttcaa cttctttttg gagcgaggct gtaatcacgc caggctgcgt
     1621 aacaacgatc gagtccgccg ccgaaaccga aagcaggcga ttcatccgcg cgagattcaa
     1681 gcaccacgat ccttcgatcg gaatcgatcc gccggctaaa cccgtcccgg cgccgcgtgg
     1741 cacgatcggg atgcgttccc gatcggcaat ccgcaagatc gaactgactt gctgcgtgga
     1801 gaggggatgc aaaacgacgg ccgggcgttg ttcagcccaa gtgccgtcgt atgcgtatgc
     1861 ggtcagcgtc gccggatcgg tggctacagc ctcagcgccg atcgcagcgc gcagttcagt
     1921 tagaagttgt ggatggcccg gttggaggtt tttgctcgcc acgatcgcgt tgatctttgt
     1981 tgtctctgtt tttgcccgtg cgtttactcg cgccacagct gcagccatgc tgcccgctgc
     2041 agggttgatc gattttcttt tggaacgctt cgagttcggc caacggttgc aggtcagcgc
     2101 gcttgacttc gatgcgcatc tcggcaactt gcactaacac cgagtcacgc agcggcaaca
     2161 cctcgatcac tttaccgcgc ccgctcggcg tcatcacttc tttg
//
LOCUS       DASRTJ010000504         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_334871,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000504 DASRTJ010000000
VERSION     DASRTJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334871"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VFF70_10195"
     CDS             <1..1554
                     /locus_tag="VFF70_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HZY45107.1"
                     /translation="AAAWNQVNVGWDKLLETVQVQTPDLAMNLLLNRWLLYQTLACRV
                     WARSAFYQSSGAFGFRDQLQDIMALIHTAPQIAREHILQAAQFQFDAGDVLHWWHPPS
                     GRGVRTRFSDDLLWLPFVVGHYVTSTNDETILDENIPFLTGPPLEPSEAERYDQYART
                     NEMYTLYEHCRRALEKGTTAGAHELPLMGSGDWNDGMNRVGIEGRGESVWLGWFLNAT
                     LKNFSKLCERRGDQTQAAGYRQRADELAKSIEASAWDGAWYRRAYYDDSTPLGSAANN
                     DCQIDSIAQSWAVLSGASDPDRATQAMESVADRLVHPSDQLVLLLAPPFNQTARDPGY
                     IKGYPPGIRENGGQYTHAALWAIWAYAELGQGDRAEMLFRLVNPIYHSDTPDKVARYM
                     VEPYVIAADVYNAPAHVGRGGWTWYTGSSGWMYRVGLEAILGIRRMGQTLHIDPCIPK
                     KWPRYQVTYRVGKTTFQISVENPMGVNRGVMQLKLDGNVLNRNTIPLLDDGSQHAVHV
                     LLGTVTATS"
BASE COUNT          370 a          415 c          473 g          346 t
ORIGIN      
        1 gcggcggcct ggaatcaggt gaatgtaggc tgggataaat tgctggagac ggttcaagtg
       61 caaacccctg atctggcaat gaacctgttg ctcaatcgct ggctgctcta ccaaacactg
      121 gcgtgccggg tgtgggcgcg ctcggcattc tatcaatcgt ccggcgcgtt tggttttcgc
      181 gatcagctgc aagacatcat ggcgctcatc catactgcgc cgcagatcgc gcgcgaacat
      241 attctgcaag ccgcacagtt tcagttcgat gccggcgacg tgttgcattg gtggcatcca
      301 ccatcaggcc gcggcgtccg cacccgtttc tctgatgatc tgttgtggct gccgtttgtc
      361 gtcggtcact atgtgactag tacaaatgat gaaacgattc ttgatgaaaa cattccgttt
      421 cttacaggcc ctccattgga gccgagtgag gcagaacgct acgatcaata tgcgcgcacg
      481 aatgaaatgt acacactcta tgaacattgc cggcgtgcct tagaaaaagg cactacggcc
      541 ggggcgcatg aactgccatt aatgggcagc ggcgattgga atgatggcat gaaccgggtc
      601 ggtatcgaag gacgcggcga aagtgtctgg ctgggttggt ttttgaatgc cacgctgaaa
      661 aatttttcaa aattatgcga gcgccgaggc gatcagaccc aagcggcagg atatcggcag
      721 cgtgccgatg agctagccaa gtcgatcgaa gccagcgcgt gggacggcgc gtggtatcgg
      781 cgcgcgtatt acgacgatag cacaccgctc ggatcggcgg cgaacaatga ttgccagatc
      841 gattcgatcg cacaatcgtg ggccgtctta tccggcgcga gtgatcccga tcgagcaacc
      901 caggcgatgg aatcggtagc cgatcggttg gtgcatccat ccgatcagct ggtactgctg
      961 ctcgcgccgc cgttcaatca aacagcgcgc gatccgggtt acatcaaagg ctatccaccc
     1021 ggcattcgcg aaaatggcgg acagtacacc cacgccgcac tctgggcgat ttgggcgtat
     1081 gccgaacttg gacaaggtga tcgcgctgaa atgctgtttc gtttggtcaa tcctatttat
     1141 cacagcgaca caccggataa agttgcaagg tatatggtgg aaccgtacgt gatcgcggcc
     1201 gatgtttaca acgcgccggc gcacgttggg cgcggcggct ggacgtggta cacgggctca
     1261 agcggctgga tgtatcgggt gggactcgaa gcaatcctgg gcatccggcg tatggggcag
     1321 acgttacaca tcgatccgtg cattccgaaa aagtggccac gttatcaagt gacatatcgc
     1381 gtaggtaaaa cgacatttca aatcagtgta gaaaatccga tgggtgtcaa tcgcggcgtc
     1441 atgcagctca aactggatgg gaacgtttta aaccgcaaca cgattccact actggacgat
     1501 ggcagtcagc atgcggttca cgtgttgctg ggcaccgtca ccgcaacaag ctagatctta
     1561 gaagccagga ctttgatgtt atgcagcaac agtggatcag ccgc
//
LOCUS       DASRTJ010000505         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_278730,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000505 DASRTJ010000000
VERSION     DASRTJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278730"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFF70_10200"
     CDS             complement(<1..342)
                     /locus_tag="VFF70_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZY45108.1"
                     /translation="MNKRERLEATIAGQTTDRTPIAMWRHFPGDDQSSDSLAEATIAF
                     QKRWDFDFVKVTPASSFQIKNWGAQDQWLGNIEGTRKYTYQPIERSSDWRSLKTLDPR
                     SGSLGNQLQCLH"
     gene            complement(420..>2315)
                     /locus_tag="VFF70_10205"
     CDS             complement(420..>2315)
                     /locus_tag="VFF70_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45109.1"
                     /translation="ESSSKITLFFIFVSALAVFLITPHSQFIWDLIPASAYIQFPWRL
                     LGPVAFTLALGGASVFSGQRLVSGDQPLPDTQHTIRNTNCSLLVTHYLPEIILLVILI
                     AALPTMYSPVWDANFGDTSPRGMIDFELSGVALGTTSTGDFQPIQIGRVPGSTQSLLD
                     SYSSNLLDKFDHASLPAGATVQVEQHSVIFDRFAIHSSIAFNARILTFYFPGWHVSID
                     GAEVPIKPADQDGFITFAVSSGAHSIEVSLQLTTPQLFGTVITLISLLALIILLLKPA
                     KASNLIAAVLPLAPVKRPRSKAEMGRPSLVPAVSPSRHLSFSPSLLLSLCILFLVGKI
                     LIIDRCDSCFRYTSPIGQALSAQYKQTSHFGGQIDLLGYDLPWMNVEAGQVLPLTLYW
                     HATTPVPVNYQVFTHLTRPDTVLWGQSDKLNPGDFPTTRWPLDKFVWDDHSLRVLPGT
                     PPGDYRLSIGLYTLDNGQRAPIFDDAGKIIGDNVVLDRVVHVDRPATPPFINSLQMHS
                     PIDQNFSGVQLLGWSIESNKLSLPNFARLTLFWRSDADRLPNLRERAELIASDDHVAQ
                     TIEAYPVQNSYPMTQWIRGEIVRDQIAFWLPPDFVIGTYSMRVSILDENSNVLKAIDL
                     AQIEVLK"
BASE COUNT          625 a          572 c          624 g          494 t
ORIGIN      
        1 atgcagacac tgcaactgat tgcccagcga gcccgatcgt ggatcgagcg tcttcaggct
       61 gcgccagtcg ctcgatcgct cgatcggttg gtatgtatac ttgcgtgtcc cctcgatgtt
      121 gcccaaccac tgatcttgcg caccccaatt tttgatctgg aaactgctgg ccggagtcac
      181 tttaacgaaa tcgaaatccc agcgtttctg aaatgcgatt gtcgcttcgg ccaaactatc
      241 ggacgattga tcgtcgccgg ggaaatgacg ccacatggca attggagttc gatccgtggt
      301 ttgccccgcg atagttgctt ctaatcgttc acgcttgttc atccaacctc gctccaaggc
      361 tcaagcgaaa gcgtcagctc tcgcgaagtt cggcagagcg ctagctctgc attgagatct
      421 cattttaata cttcaatttg tgccagatcg atggccttca acacattact gttttcatcc
      481 aagatactga cgcgcatcga atatgtgcca atgacaaaat ccggcggcag ccaaaacgcg
      541 atttgatcac ggacgatctc gccgcgtatc cactgcgtca tgggatacga attctgaact
      601 ggatacgctt cgatcgtttg cgcaacatga tcatcgctgg caatcaactc ggcgcgctcg
      661 cgcagattcg gcagccgatc ggcatccgat cgccagaaca gtgttaaccg cgcgaaattc
      721 ggcagtgaaa gtttattcga ctcgatcgac cagcctagca gctgcacgcc gctgaagttt
      781 tgatcgatcg ggctatgcat ttggagtgaa ttaatgaatg gaggcgtagc gggtcgatcg
      841 acatgcacga ctcgatcgag cactacatta tcgccaataa tcttccccgc atcatcaaag
      901 atcggcgcgc gttggccgtt gtccaaagtg tacagaccga tcgataggcg ataatcgccg
      961 ggcggtgtac cgggcaacac gcgcaatgaa tgatcatccc agacgaactt atccagcggc
     1021 cagcgcgtcg ttgggaagtc acccggattc agtttatcgg attgccccca caatacggtg
     1081 tcaggccgag tcaagtgcgt aaaaacttga tagttgaccg gcacaggtgt ggtcgcatgc
     1141 caataaagag tcaaaggcaa aacttgtcct gcctcaacgt tcatccatgg cagatcgtaa
     1201 cccaatagat cgatctgtcc gccgaagtgc gaagtctgct tatattgtgc gctgagagct
     1261 tgtccgatcg gcgaagtgta gcgaaagcaa ctatcgcagc gatcgatgat cagaattttg
     1321 ccgacgagaa agagaatgca aagcgagaga aggagagagg gagagaaaga gagatggcga
     1381 gatggagaga cggcgggaac aagcgagggg cgacccatct ccgctttgct acgagggcgc
     1441 ttcacggggg caaggggcaa gaccgcggct attagatttg aggctttagc cggtttgaga
     1501 agtaatatga ttagggctaa caaagatata agcgtgatga ctgtgccgaa taattggggt
     1561 gtcgttagtt gtaacgaaac ctcgatcgaa tgcgcaccac ttgacacagc aaaagtaatg
     1621 aagccgtctt gatcggcagg cttgatcgga acttcagcgc catcgatcga tacatgccag
     1681 cctggaaaat aaaaggtcag gatccgcgca ttaaaagcga tcgatgaatg gattgcaaac
     1741 cgatcgaaga taaccgagtg ctgttcaact tgaactgtcg cgccagcggg caatgaagcg
     1801 tgatcaaact tatcgagcaa attagaagaa tatgaatcga gcaaagattg agttgacccc
     1861 gggacacgtc cgatctggat cggctgaaag tcaccagtcg aggtagtgcc cagcgcgacg
     1921 ccactcaatt caaaatcgat catgccgcgc ggcgaagtat ccccgaagtt cgcgtcccat
     1981 acaggtgaat acatggtggg caaggcggcg atgagtatca ctaacagaat gatctcaggc
     2041 agataatgag taacaagtaa tgagcaattc gtattgcgga ttgtgtgttg cgtatcaggc
     2101 aacggctggt cgcccgacac taggcgctgg ccactgaaaa ctgatgcccc acccagcgcc
     2161 aatgtgaagg caaccggccc cagcaaccgc cacgggaatt gaatgtaagc cgaagccgga
     2221 atcaagtccc aaatgaattg cgagtgcggg gtgatcaaga aaaccgctaa agctgaaaca
     2281 aaaataaaga atagagtaat cttgctggaa gattc
//
LOCUS       DASRTJ010000506         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_106959,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000506 DASRTJ010000000
VERSION     DASRTJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106959"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFF70_10210"
     CDS             <1..848
                     /locus_tag="VFF70_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45110.1"
                     /translation="VLAEEDAAQSLKEHFEAAGQTVLVAHSQDEALAAVRQWLPQAVI
                     LDMETPGLNVTDLCHNIRSTPRTRHIHITLLALQTRRDDRLLALSSGADEFLTKPVDA
                     EELGLRIRNALRRAEYQNLVNPTTGLPGQQLIEDRLRELLRSENEWALIRSNLRGFKA
                     FTDAYGFLAREVVLRFAVQVFTQTVNQLGTLDDFLGHTGSDNFIVVTALDRSDALRQT
                     LSDRFAEGVRSHYSFHERELGYMIVRLSDGGETKAPHMSLNTQMITSKQGPFYDIFEL
                     TQMQS"
     gene            complement(895..2031)
                     /gene="solA"
                     /locus_tag="VFF70_10215"
     CDS             complement(895..2031)
                     /gene="solA"
                     /locus_tag="VFF70_10215"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HZY45111.1"
                     /translation="MTIDFETIIIGAGAMGSAAAYHLARDNRSVLLLEQFASGHTRGS
                     SHGDSRIIRLAYDHPIYVELARSAYHLWAELESELHRALITTTGGLDMSAPDQPSFKL
                     RVDALQQTGVPFEILDAAEIMRRFPQWHLSEKTVGVYQADSGILNPGECVPLIAEQAM
                     RRGAVLLDQTRVRLIHSIDHGVEVVTDGQSYKCKKLIISAGAWTGSLLKSIGVNLPLV
                     VTQEQFAYYAVHPPADFQPDRFPVFIHYGSGGSFDNYGLPIFGHAGVKVGEHHAGPVV
                     TADTRSFEIDQMRLERLTHYVRMHLPDTTGEAFGVTSCLYTNTPDEHFIIDILPDHPQ
                     VIVASPCSGHGFKFSILIGRILADLVERGKTDYPIGMFGLKRFL"
     gene            2196..2477
                     /locus_tag="VFF70_10220"
     CDS             2196..2477
                     /locus_tag="VFF70_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZY45112.1"
                     /translation="MSEAIARLHVTIRGRVQGVGFRFHTQQAAEELGLTGWVANRSDR
                     SVEVVAEGPRAALDRFLTTLKHGPSMAQVIDLQPEYLPATREFAEFRVE"
     gene            2527..>2988
                     /locus_tag="VFF70_10225"
     CDS             2527..>2988
                     /locus_tag="VFF70_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45113.1"
                     /translation="MRRNPERLAWTVLGISLLICVTLAIAVPLAIRSFINDTTDISRI
                     KLEVQQGTVLVRPLGTDDPIGVTTQLDNLLEGVTIRADENVQALLTIESPNNGATLMI
                     VQIYGSTNLAITLARTPRFQASGLPHSETLTIDGGRVRVTVSPDGARSTDAR"
BASE COUNT          687 a          813 c          798 g          690 t
ORIGIN      
        1 ttgtgcttgc ggaggaagac gcggcacaat cattgaagga acattttgaa gcagctggcc
       61 agaccgtgct ggtggcccac tcgcaagacg aagcgttggc tgctgtgcgc caatggctgc
      121 cgcaagcggt gattctcgat atggaaacac caggtctgaa tgtcacggat ctgtgtcaca
      181 acatccgatc aaccccgcgc acccggcata tccacatcac cttactcgcg ctgcaaaccc
      241 gccgcgacga tcgattgttg gcgctgtcgt ccggcgcgga tgaattcctg acaaagcccg
      301 tcgatgccga agaattgggg ctgcgcattc gcaacgcgct gcgccgcgcc gaatatcaga
      361 acctagtgaa tccgacgacc gggttgcccg gtcagcagtt gatcgaagat cgattgcgcg
      421 aactactgcg cagcgaaaac gagtgggcct taatccgatc gaacctgcgc ggtttcaagg
      481 cctttaccga tgcttacgga tttttagccc gtgaagtcgt gctgcgtttt gccgtgcagg
      541 ttttcactca aaccgttaat cagttaggca ctctagatga ttttctgggg cataccggca
      601 gtgacaactt cattgtcgtc accgcgctcg atcggtcaga tgcgctgcgc caaaccttga
      661 gcgatcgctt cgctgaaggt gtccgctcgc attattcatt ccacgaacgc gaactgggtt
      721 atatgatcgt gagattgtcc gacggaggag agaccaaagc cccgcacatg tcattgaata
      781 cacagatgat tacctcgaag caagggccgt tctacgatat ttttgaacta acccagatgc
      841 aatcataatg agtgatgagt aatgagtaaa agattactcg ttccctgtta ttcgttaaag
      901 aaacctcttc aacccaaaca tcccgatcgg ataatccgtt ttacctcgtt cgaccaagtc
      961 tgccagaatc ctgccgatca agatcgaaaa tttaaagccg tggccggagc agggcgaagc
     1021 cacgatgacc tgtggatgat ccggcaggat gtcgatgatg aaatgctcgt cgggcgtatt
     1081 ggtatacaag caactggtca caccgaacgc ttcacctgtg gtatcgggta aatgcatgcg
     1141 cacgtagtga gtcagccgct cgagccgcat ctgatcgatc tcgaacgatc gtgtgtctgc
     1201 ggtaacgacc ggcccagcat gatgctcacc aactttcacg ccagcatgcc cgaagatcgg
     1261 caagccataa ttatcgaacg atccgccgga accgtagtga atgaacaccg gaaatcgatc
     1321 cggttggaag tctgctggtg gatgcacggc gtagtaagca aactgttctt gcgtcacaac
     1381 caacggtaaa tttaccccga tcgattttag cagcgatcca gtccacgcgc cggcgctgat
     1441 aattaatttt ttgcacttgt agctctgtcc gtcagttaca acttcgacgc catgatcgat
     1501 gctgtgtatc aatcggacgc gcgtttggtc gagcagcact gcgccacgcc gcatggcttg
     1561 ttcggcaatc agcggcacac attcgccggg attcaagatg cccgaatcgg cttgatacac
     1621 gccgaccgtt ttttcgctca agtgccactg tggaaagcgg cgcatgattt cggccgcatc
     1681 caaaatttcg aacggtacac ccgtctgctg taaagcatca acacgcagtt tgaatgaagg
     1741 ctgatcgggc gcggacatat ccaacccgcc ggtcgttgtg atcaatgcgc ggtgaagttc
     1801 actttccaac tctgcccaca agtgatatgc tgatcgagcc aactcaacat agattggatg
     1861 atcgtaagcc agccggatga tgcggctgtc accgtgtgag ctgccgcgag tatgcccgct
     1921 ggcaaattgc tcgagcagca agaccgaccg gttgtcccgg gctaaatgat aagccgccgc
     1981 gctgcccatt gcgcctgcgc caatgatgat cgtttcaaaa tcaatcgtca ttgtctgacc
     2041 tcataagttg atttgatatt gcaaatcgaa tatcgcacag ctcacagcat cacttgcgac
     2101 ttgcaatctg catcttttcc ttcgtgtgcg tatgcgcctt gccccttgcc cttcgctatg
     2161 gtattatcgc gcgcgttgct tgagtggaga aggaaatgag tgaagcaatc gcgcgattgc
     2221 atgtgacgat tcgcggtcga gtgcagggtg tgggtttccg ctttcacacg cagcaagccg
     2281 ccgaagaatt gggcctcacg ggctgggtgg ctaatcgatc ggatcgcagc gttgaggtgg
     2341 tagccgaagg cccgcgtgcc gccctggatc gatttttaac aactttgaag cacggtccgt
     2401 cgatggctca agtgatcgac ctgcagcctg aatatttgcc ggcaactcgc gagttcgctg
     2461 aatttcgcgt tgaatgatca ggattgtccg cagccttgat tttgaagcgt tggttcacac
     2521 actaatatgc gacgcaaccc cgaacgttta gcctggacgg ttctcggcat ttcattgttg
     2581 atctgcgtca ccttggccat cgctgtgccg ctggcgatcc gcagcttcat taacgacact
     2641 accgatattt ccagaatcaa actggaagtg cagcaaggta cggtcttggt gcggccactg
     2701 ggcactgatg atccgatcgg tgttaccacc caactggata atctcctgga aggcgttacg
     2761 attcgagccg acgaaaacgt gcaggcattg ctgacgatcg agtcgccgaa caacggcgcg
     2821 acgttgatga tcgttcagat ctacggcagc accaatctgg cgattacctt agcccgaacg
     2881 ccgcgctttc aagccagcgg tctgcctcac agcgaaacgc taacgatcga tggcggccgc
     2941 gtgcgtgtga ctgtttcacc ggatggcgcg cgatcgacgg atgccaga
//
LOCUS       DASRTJ010000507         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_69966,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000507 DASRTJ010000000
VERSION     DASRTJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69966"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1450
                     /locus_tag="VFF70_10230"
     CDS             119..1450
                     /locus_tag="VFF70_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45114.1"
                     /translation="MSPEFDWQVQDDHGQEEIVHTPQPRSPRWRPIAIVVVIAIGVAL
                     GIAYRSIPEPPKTPTPTPTIVALPPLIAEVVDREVQALANGAKDAFLSIQDPNDANWY
                     QAQADAFETWGQAPATNPEYTVIETGTLADNRVWVDVRQWHGEFSYVRETRFYRLRDN
                     RWLRTRPDVSFWSGQDARLSTTHFEVIYPKEDLSLAQIIVDRFERAYVAVCGDLHCEI
                     GPTSRVLPQVLTLTLVLTQTDTFTTIDQGQYVTITFPSPRMLRIADRWGVTDDPLDAM
                     AIAQMAVPVARIVSGGADRWFKSGDGVLFFNAIAEWERPRAKSVTSEFFVIDNRTNQL
                     IRLTAHENNLGQSQQTYIDQLRDAQLVPLKALWSWPLNMPPGSSMFDTMKIEADAMIG
                     FIQFRYKPDGVARLLLALKLAQSLPEAIETSLGTKYEDFEAEWLKWIGKAE"
     gene            complement(1483..2118)
                     /locus_tag="VFF70_10235"
     CDS             complement(1483..2118)
                     /locus_tag="VFF70_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45115.1"
                     /translation="MRANITKIFLVMLLCSIVGVAPVAAQLPGPNPQLSITASPFPLM
                     INQNARITMVATNVGTANADNIVISNGVPNNMGIIGVATSQGGASVYNSAATIDIGTL
                     APGQSATVYMDVFVVQANPSDTPFNDCAGLTFYNGTARLSCLPRQSAINKTPRVSYSL
                     PPNGARPIDNPNRGPILLPVAGGALIDSSAVLLVLGGVSIVLGRLLKRKRE"
BASE COUNT          511 a          575 c          628 g          471 t
ORIGIN      
        1 ctcggcccgg ccacctcact gcgggatgcg atcgagtcca cgggattggt ttacaatgac
       61 ttcgagaaga actggcagac gtggctgaac aagaattttc cgaagtaaga tcgatgcgat
      121 gagtccagag ttcgattggc aagtacaaga cgatcacggc caggaagaga tcgttcatac
      181 gccgcagcct cgatcgccgc gctggcggcc gatcgcgatt gtggtagtca ttgcgatcgg
      241 cgtcgcgttg ggcattgctt atcgatcgat ccctgaacca ccgaaaaccc ccacgccgac
      301 accgacgatc gttgcgctgc cgccgctgat tgccgaagtg gtcgatcgag aagttcaggc
      361 cttggcgaac ggcgctaaag atgcattcct atcgattcaa gatccaaatg atgcgaattg
      421 gtatcaagcg caagccgatg cctttgagac gtggggccaa gcgcccgcaa ccaatcctga
      481 atataccgtg atcgaaacgg gcacgctggc ggataatcgc gtgtgggtgg acgtgcggca
      541 gtggcatggt gaattcagtt acgtgcgcga gacgcgtttt tatcgattgc gcgataatcg
      601 ctggctgcgc actcggcctg atgtgtcgtt ttggagcgga caagatgcgc ggctgtcgac
      661 aacgcatttc gaggtgatct atcctaaaga ggatttatcg ttggcgcaga tcatcgtcga
      721 tcgcttcgag cgcgcgtatg tcgcagtctg cggcgatctg cattgcgaga tcggcccgac
      781 aagccgcgta ctgccgcagg ttttaacact gacgctggta ttgacgcaga ctgatacttt
      841 caccacgatc gatcaaggcc aatatgtgac gatcaccttt ccctcaccac ggatgctgcg
      901 cattgccgat cgctggggcg taacggatga tccgcttgat gccatggcga ttgctcagat
      961 ggccgtgcca gtggcgcgca tcgtgtctgg tggagccgat cgctggttca agtccggcga
     1021 tggggtgtta ttcttcaacg cgatcgcgga atgggaacgt ccgcgtgcaa agtccgtgac
     1081 gagcgaattt tttgtaatcg acaatcgaac caatcagctg atcagactca ccgcacatga
     1141 gaacaacctc gggcaatcgc aacagactta catcgatcaa ctcagagacg cgcagctggt
     1201 accgttgaag gcgctgtggt cgtggccgtt gaatatgcca ccgggcagca gcatgtttga
     1261 caccatgaag atcgaagcag atgcgatgat cggtttcatc caatttcgat acaagccaga
     1321 cggcgtggcg cgtctcttat tagcactgaa gttggcgcag tccttgccgg aagcgatcga
     1381 gacaagtttg ggaacgaagt acgaagattt cgaagcagag tggttgaagt ggattggaaa
     1441 agcggaatga gagatggagt gaaggagaga aaaagcaatc gctcattctc tctttctttt
     1501 caataaacgc cccagcacga tcgacacccc acccagcacc aacaacaccg cactcgaatc
     1561 gatcaacgct ccacctgcaa ccggcagcag gattgggccg cgattcggat tatcgatcgg
     1621 gcgcgcgccg tttggtggta aagagtaact cacccgcggg gttttattaa tggccgactg
     1681 gcgtggcagg cacgataagc gagcggtgcc attatagaag gtcaagcccg cgcaatcatt
     1741 gaacggcgta tcgctgggat tggcctggac gacaaacaca tccatataca ccgtcgcact
     1801 ttggccgggc gcgagcgtgc caatgtcgat cgtcgccgcc gagttgtaaa cgctggcccc
     1861 gccttgcgaa gtggccacgc caatgatgcc catgttattc ggcacgccgt tagagatgac
     1921 aatgttatcg gcattggccg tgccgacatt ggtggccacc atggttatgc gtgcattctg
     1981 attgatcatc agtggaaacg gcgaagcggt aatcgatagc tgcggattcg gcccaggtaa
     2041 ctgcgccgcc acgggcgcca ccccaacgat cgagcataac agcatgacca gaaatatttt
     2101 cgttatattt gcgcgcataa gagatcgcct tgaataatcg ttcagctccg tatgaatctt
     2161 ttcaaccctt cgctgcatcg gtgga
//
LOCUS       DASRTJ010000508         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_107127,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000508 DASRTJ010000000
VERSION     DASRTJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107127"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFF70_10240"
     CDS             complement(<1..1239)
                     /locus_tag="VFF70_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZY45116.1"
                     /translation="MFLSPNRILKDRYRILSLLGQGGMGSVYRGFDTALDRPVAVKQL
                     LPAMASDATTTAAIKQQFEREAKILAALTHPNLPRVTDYFTDDNMPYLVMDYVEGDTL
                     SDLLKQKRNGFPEIQVLNWADQILSALDYIHAHGVIHRDIKPGNLRLTPDGRIFLVDF
                     GLSKSLQSEHPETATMMRGVGTAEYAPPEQYDSTLGHTDARADIYSLGATLYHLLVGK
                     APATVTQRMAEPQSFQPMRDTGALISPQIDRVINRAMEIERAKRFASAADMRAALKLA
                     APDRSTGSISTRLLGNVTSQPKRSTGRIALIGLISVALLAAAIVIVVTSSPSTRPPST
                     PTATTPIQPDVRIVTLSGTSTPEYVDIKNFGGVTQTLTGWKLVSTEGQQTFNFPNGFA
                     LPPGASVRLSSYTGAINNPPT"
     gene            complement(1448..1777)
                     /locus_tag="VFF70_10245"
     CDS             complement(1448..1777)
                     /locus_tag="VFF70_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY45117.1"
                     /translation="MIDRGQVIVEPGVTLGYYAQQLVTLDRSQPLIDVIRQAKQFGEP
                     EAATLLIEILFSDERCCQRVNELSSGERCRLQLAPLMISTANFLLLDEPRHTTSKSLQ
                     PNCWKKS"
BASE COUNT          461 a          509 c          582 g          481 t
ORIGIN      
        1 ggtcggtgga ttgttgatcg cgcccgtata actgctcagt cttacgcttg cgccaggcgg
       61 aagtgcaaag ccattaggaa aattgaaagt ttgctgacct tccgtgctca ctaatttcca
      121 gcctgtcaac gtttgtgtga cgcccccgaa atttttgatg tccacatatt caggagtgct
      181 cgtgccgctc agtgtgacga tgcgcacgtc cggttgaatt ggagttgtcg cagttggcgt
      241 cgatggtggg cgcgtcgatg gactcgatgt cacgacgatc acaatcgcgg cggcaagcaa
      301 tgccacactg attaacccga tcaatgcaat acggccagtc gatcgtttcg gctgagaagt
      361 gacattgccc agcaagcgtg tgctaatgga accagtagat cgatcgggag cagcgagctt
      421 caacgcggcg cgcatatcgg cggctgaggc aaagcgcttg gcacgctcga tctccatcgc
      481 acggttgatc actcgatcga tctgcggtga gatcagtgcg cccgtatcac gcataggttg
      541 aaacgactga ggctcggcca tgcgctgggt gacggtggcc ggtgctttgc caacgagcag
      601 atgatacaac gtcgcgccga gtgaatagat gtctgcacgg gcatcggtat gaccgagcgt
      661 gctgtcgtat tgttcgggcg gcgcatattc ggccgtaccc acaccacgca tcatcgtcgc
      721 tgtctcagga tgttccgatt gcaacgactt cgacaaacca aagtcgacga ggaagatgcg
      781 gccatccggc gtgaggcgca ggttgccggg tttgatgtcg cgatgtataa cgccatgtgc
      841 gtgaatgtaa tcgagcgccg ataaaatctg atcagcccaa ttgagcactt gaatttcggg
      901 aaagccgttg cgtttctgtt tgagcagatc gctaagcgtg tcgccttcga cataatccat
      961 gacgagatac ggcatgttgt cgtcggtgaa ataatcggtg acgcgcggca gattaggatg
     1021 tgtgagtgct gccaaaattt tggcttcacg ctcgaactgt tgcttgatcg cagcagtcgt
     1081 tgtcgcgtcg gacgccatgg cagggagcag ctgcttcacg gcgacaggtc gatccagtgc
     1141 ggtatcgaaa ccacggtaga ccgagcccat gccgccttga cccagcaaac ttaaaattcg
     1201 atagcgatct ttgaggatgc gattgggtga taggaacatg atctctaccg gttcatgttc
     1261 agaatgaatt tagtatagca caactgccga catttacggt atcaagtgag tttcgattca
     1321 tcttagtccg agaaaccgcc cagttgtccc accttttgtc cgcaatccaa ttctacggcg
     1381 cggtgggtga tgcgatcgag accatagcgt tcgggcgata cgcacaatac gctgccttcg
     1441 agattgttta agacttcttc cagcagtttg gctgaagcga tttcgaggtt gtatgtcggg
     1501 gttcgtcgag caacaggaag ttggcggtgc tgatcatcaa tggtgccaac tgtaagcgac
     1561 accgctcacc actcgatagt tcattcacgc gttgacagca ccgctcatca ctgaacagaa
     1621 tctcgatcaa gagtgtggcc gcttcgggtt cgccgaattg tttggcttgc cggatcacat
     1681 cgatcaacgg ctgagatcga tcgagtgtca caagctgctg ggcgtaatac ccgagcgtga
     1741 cgcctggctc gacgatgact tggccgcgat cgatcatcaa ccgcagctag tgtcacttat
     1801 ggataaccac gcaaatgaag aggggatagc cacccggttg gcatcggcgc gaacgggcag
     1861 ggcgataaag tattaacgac gcagaccggc tggcgctaag tgaaatggag tatatttctt
     1921 cgagggttcg cgaatttgat atcagctgcg cgcttgcaag atgttcctga agttccaggc
     1981 ggaataatcc gggaacactt cttcgatctt gctattcagt aaccgtttct gca
//
LOCUS       DASRTJ010000509         8070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_335097,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000509 DASRTJ010000000
VERSION     DASRTJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335097"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFF70_10250"
     CDS             complement(<1..654)
                     /locus_tag="VFF70_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZY45118.1"
                     /translation="MKRLMLRLILGLVFAIPLMLVATIWVQASPIVPAPVKEASLSNC
                     VVCHAEYQNAWEHGAHGQAATNGNFTTAWKAQGSPAECMSCHATGYDANTQTWKTEGV
                     TCEACHGPIASDHPKTPMPVDRTGKTCGNCHTETYFEWQVSKHRANDLACSSCHDAHA
                     TKLKATDPSTLCATCHQERASNFSHTAHSAKGLNCADCHLSPLDSKLGQGHGERDHSF
                     "
     gene            complement(714..1058)
                     /locus_tag="VFF70_10255"
     CDS             complement(714..1058)
                     /locus_tag="VFF70_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45119.1"
                     /translation="MTLNGFAVVLLGILVRFLLPIAATLLVIWFFRKLDARWQIEAQQ
                     QLHAQMALAVANHTPCWEQRQCSAEKRALCPAYQNSYRPCWQVFRDTDGRLREACMEC
                     DVFLSAPVPAIL"
     gene            complement(1526..2176)
                     /locus_tag="VFF70_10260"
     CDS             complement(1526..2176)
                     /locus_tag="VFF70_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45120.1"
                     /translation="MTIKILLVDDHAVVRSGLRMLLENETDVEIVGEAATGREALALV
                     DRLKPDMVIMDIGLPDITGIEATQKIKQTSPQVAVVALTIHEDEEYFFKMLQAGANGY
                     VPKRAAPEELLTAIRIAAAGGVYLYPSLAKLLVRDYLSSDHDASKKDALGELTDRERE
                     VLTHLADGADTQSIAEALSISPKTVARHRDNIMNKLNLHSRTELVKYAIRKGIIQA"
     gene            complement(2290..3792)
                     /locus_tag="VFF70_10265"
     CDS             complement(2290..3792)
                     /locus_tag="VFF70_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZY45121.1"
                     /translation="MSGIDNTYLVLVYFVYGLAFFSLGLAVILERGRGSDVRLRHALR
                     PLAAFGLLHGTHEWIEMLEQMRALPGQFTVPIAWDGIRLGILCFSFLSLSAFGASLLS
                     PTERQRRLSLLVPLFQVVMWSVGLLIFRGEYIYTSRLMDVAEVWTRYVVAIPAALLAS
                     VGLVAQQRAFRQAGMIRFGRDSLWAAVAFAWYGIIGQVFVRPSPLPPSDVFNQELFLR
                     LFGFPVQLLRAIAALMISLFIIRFFRSFEVETQQRIAELQAGRLEEAERREALRGELL
                     RRVVAAQEAERQRIARELHDATGQALTALGLGLRGVATSYARQDNDQIDKRLRQLEDL
                     TTRSLDELRHLIADLRPSHLDDLGLAAALRWYAKDLQSRVPLDIRVEINGEERPLASA
                     VKIALFRVAQEALTNIIKYADAEHAWVRLNYADQEVMLQIEDNGRGFDMEAQANSARS
                     QWGLLGMQERAALLGGRFDLYAVAGQGTRVQVVIPYQQVFEEYVAAARLT"
     gene            3986..4888
                     /locus_tag="VFF70_10270"
     CDS             3986..4888
                     /locus_tag="VFF70_10270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZY45122.1"
                     /translation="MLNKIYAHSCEAMTELNNDSVALTVTSPPYWNAIDYEVHSIDKQ
                     KNYRTRKYALGYTEYEEYLTWLDRIFSEVLRVTQPGGFCAIVVGTVLLNSRHYPVPFD
                     LIARLTRSGWLFHQDIIWHKCTAGVKRAGVTIQKPYPGYYYPNIMTEYILIFRKPGDP
                     IYKTHSLKDKHSAQFPINRVFTMDTANNLWHIAPVPPDLIDHPCPYPEEIPYRLIQMY
                     SYPGEVVLDPFVGSGQTLKVAKWLDRAYVGYETIRKYVNLANKRLKQKLSIRPKQLIA
                     RFEKIDLDEPAGQTPTTRSRRVKS"
     gene            complement(4986..6314)
                     /locus_tag="VFF70_10275"
     CDS             complement(4986..6314)
                     /locus_tag="VFF70_10275"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HZY45123.1"
                     /translation="MNPIFPKDFVWGAATASYQIEGAWNEDGKGESIWDRFSHTLGKV
                     ENGDTGDMACDHYHRWRDDLVLMKQLGLKAYRFSISWPRVIPNGFGQINPLGLDFYDR
                     LVDALLDADIQPFATLYHWDLPQALQDRGGWSNREVSAYFADYAAAVVRRLGDRVRSW
                     ATFNEPWVVAFVGNRDGRHAPGLTNEQLALQVAHHLLVAHGLAAQAIRSIDPALEVGI
                     VNNMWPYEFEIDTPENRSQADELWLKNEGWFIEPIVCGSYPARAWAAYGANVPIIQPG
                     DMALIAQRIDFMGINYYSRHLVENGEIVANVPNVEHTEMGWEVNAPGLRRLLNQLKRD
                     YRLPPLYITENGAAFVDQVSPDGQVHDPRRLNYFREHIRQIKLAIDDGVNLRGYFAWS
                     LLDNFEWGYGYSKRFGLVHVDYQTQQRIIKDSGLWYKQVIARNGVADESL"
     gene            complement(6316..7227)
                     /locus_tag="VFF70_10280"
     CDS             complement(6316..7227)
                     /locus_tag="VFF70_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45124.1"
                     /translation="MLTGLERVTGDFHGLPTHILGNDQLSLEFLSEAGPRIVRLKLPG
                     NDQNFLAEVPDFKWTTPHGDYFLHGGHRLWRAPEIFPQTYMPDSSGLKIEELSNGVRL
                     SRAAQLTGDIRKSIEIQLAPDRPAVTLIHQLTNDSDRSIECAPWAITQLALGGIAIMP
                     LPHDSVEADELRPNRHLTLWPYTRWSDSRLHVDDDGVLIEARSELPALKVGSLNRHGW
                     IGYLIGGVLFRKCFEPATDQLYPDLGSNVEIYCNDRFMELETLGGLRPLQPGQTATHI
                     ETWELLTGLDVPATLAGAQALLKKLES"
     gene            complement(7233..>8070)
                     /locus_tag="VFF70_10285"
     CDS             complement(7233..>8070)
                     /locus_tag="VFF70_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZY45125.1"
                     /translation="HLSAEAQQCLPEAVKMKTTVDQYGWDGDWFLRAYDNFGAKIGSH
                     ECDEGRIFIEPQGMCIIGGIGLEDGRAERALAAVNQHLATQHGIILQQPAYSHYYLNL
                     GEISSYPPGYKENAGIFCHTNPWIIIAEAIIGHGDQAHAYYSRINPSAREAISEVHRC
                     EPYVYAQMIAGRDAPTHGEAKNSWLTGTAAWNYAAISQWILGIRPSLGGLLIAPVIPT
                     KWPGFSATRVFRGVTYRIEVKRVGRGNNVSLRVNGELIAGNVVSLPADGQAEVSVEVT
                     LN"
BASE COUNT         1897 a         2375 c         2060 g         1738 t
ORIGIN      
        1 gaaactgtga tcgcgctcgc catgcccctg ccctagtttg ctatccagcg gcgatagatg
       61 gcaatccgca cagttcagcc ctttggcaga atgcgcagtg tgcgagaaat tactggcgcg
      121 ttcttgatgg cacgtcgcgc acagcgtcga tggatcagtt gccttcaact ttgtagcatg
      181 cgcatcgtgg cagctggagc aggccagatc attggcgcga tgcttgctca cttgccactc
      241 gaaataagtt tcagtatggc agttgccgca ggtcttgccg gttcgatcga ccggcatggg
      301 cgtcttcgga tgatcggacg cgatcggccc gtgacacgct tcgcacgtta cgccttcggt
      361 cttccaggtt tgggtgttgg catcgtaacc ggtcgcgtgg cagctcatgc actccgccgg
      421 gctgccttgc gccttccaag cagtcgtaaa attgccattg gtggctgctt gtccgtgcgc
      481 accatgctcc cacgcgtttt gatattcggc gtggcagacc acacaattgg acagcgacgc
      541 ttctttaacc ggcgcgggaa cgatcgggct ggcctgcacc caaatagtcg ccaccagcat
      601 caatggtatt gcaaaaacaa gtcccaatat caagcgcagc atcaagcgtt tcatttcaaa
      661 atcctcctag cctaaacttc cgaagtctct caggcttcgg aagattcaat gatctaaagt
      721 atggccggca ccggcgcaga caggaacaca tcgcattcca tacacgcttc acgcaagcgg
      781 ccgtctgtat ctcgaaagac ttgccagcac ggcctgtagc tgttttgata ggccgggcag
      841 agtgcgcgtt tttccgccga gcactggcgc tgctcccagc acggcgtgtg gttcgcaaca
      901 gccaaggcca tctgcgcatg caactgttgc tgcgcctcga tctgccagcg ggcatcaagt
      961 ttgcggaaga accagataac cagcagcgtc gccgcaatcg gcagaaggaa gcgcaccaga
     1021 atgccgagga gaacgactgc aaatccgtta agagtcatgt cacaccgcct aatggctgtg
     1081 ccgcgtgttt tgatagcgag cgatcaaagt gcccacaacc agcagcacca caattggcag
     1141 gacaattcgc aacatcgtta aagtgagaat gacagttgtg ccgaacatga tccacctcct
     1201 gctccaatct caatcgatcc tctttctcga tcttcacttg cagtatagag atcgatctac
     1261 tcgatcacaa tccgtcattc acccgatctt tgtctgcgcg atctagcagg tgatcgaacg
     1321 agcgcatggg gtttttaccc atgtccgatt gggtggatca ccccagcgat taagacgaag
     1381 cggctcagcc taaaggagtc gaggctttag ccggttaatg tgactaaaac atccggctaa
     1441 agcctcgaca ccagaataaa aaaagggcga gcagccgctc gcccctactt cttcaattca
     1501 tccttccacc ctcagccttc agccactacg cctgaatgat tcctttgcgg atcgcgtact
     1561 tcaccaactc tgtgcgcgaa tgcagattca atttattcat gatgttatcg cgatgccgcg
     1621 ctacagtttt cggactgatg ctcaacgcct ccgcgatcga ttgggtgtct gcgccgtcgg
     1681 ccaagtgcgt cagcacttcg cgctctcgat cggtcaactc gcccagcgcg tcttttttcg
     1741 aggcatcgtg atcactcgac agataatcgc gcaccaagag cttggccaat gaaggataca
     1801 gatagactcc gcctgcagct gcaatccgaa tcgccgtgag caattcttcg ggtgcggcgc
     1861 gcttgggcac atagccgttt gcgccggctt gcagcatctt gaagaaatac tcttcatctt
     1921 cgtggatcgt caacgcaacc acagccactt gcggcgaagt ctgcttgatc ttctgcgtcg
     1981 cctcaatgcc agtgatgtcc ggcaggccga tgtccatgat aaccatatcg ggtttcaagc
     2041 gatcgacgag cgccagtgct tcgcggccgg ttgccgcttc accgacgatt tcgacatcgg
     2101 tttcattctc caacagcatc cgcaggcccg atcgaaccac ggcgtgatcg tcaaccaata
     2161 atattttgat ggtcatacga actccttgtc agtcagggcg acacattcgc caacaaatca
     2221 gtattgattg atactcacgc tgaggccagg ttgcgtgttg gctcaaatca ttgagcgagt
     2281 gcgtcgcccc tacgtcaatc tggctgctgc gacatattct tcaaaaacct gttgatacgg
     2341 tatcaccact tgcacgcgcg tgccctgtcc cgcgactgca tagagatcga atcgaccacc
     2401 aagcagcgcg gcacgttcct gcatgccgag cagtccccac tgtgagcgcg ccgaattggc
     2461 ctgtgcctcc atatcgaagc cgcggccgtt gtcttcaatc tgcagcatca cttcttgatc
     2521 ggcgtaattc aagcgcaccc aggcgtgctc ggcatccgca tacttgatga tgttcgtcag
     2581 cgcttcttga gccacgcgga aaagagcgat cttcaccgct gaggccagcg gccgctcctc
     2641 gccattaatc tcgacgcgaa tgtcgagcgg cacgcggctc tgcaaatctt tggcatacca
     2701 acgcagcgct gccgccaaac ccagatcgtc aaggtgcgac ggccgcagat cggcgatcag
     2761 atgccgcagc tcatccaagg atcgtgtcgt cagatcttcc aactgcctca gccgtttatc
     2821 gatctgatcg ttgtcttgcc gcgcgtaact cgtcgcgacg ccgcgcaagc caagccctag
     2881 tgcggttagt gcttgcccgg tggcatcatg caattcgcgg gcgatgcgct gccgctcggc
     2941 ctcttgggcc gcgaccactc gtcggagcag ctcgccgcgc aaagcttcgc gccgttcggc
     3001 ttcttccaat cgaccagctt gcagttcggc aatgcgctgc tgcgtttcaa cttcgaatga
     3061 tcggaagaag cgaatgatga acaaggaaat catcagcgcc gcgatcgcgc gcagcagttg
     3121 aaccggaaat ccgaacagcc gcaaaaacaa ttcctgattg aaaacatccg agggcggcag
     3181 cgggctcggt cggacgaaca cctggccgat gatgccatac cacgcaaaag cgaccgcggc
     3241 ccacaaacta tcgcggccaa aacgaatcat gccggcttgt cggaaagcac gctgctgcgc
     3301 caccaaaccg acactggcca acaaggcggc cgggatcgcc acgacgtagc gcgtccagac
     3361 ttcggccaca tccattaagc ggctggtgta aatatattcg ccgcgaaaga tcaacaggcc
     3421 cacgctccac atcaccactt ggaataacgg gactaacaaa ctcaatcgcc gctgacgttc
     3481 ggtcgggctg agcagcgacg cgccaaatgc gctcaatgat aagaacgaga aacacaggat
     3541 acccaagcga atgccatccc acgcgatcgg caccgtgaac tgaccgggca gcgcgcgcat
     3601 ctgctccagc atctcgatcc attcatgcgt gccgtgcagt agaccaaacg cggctaacgg
     3661 gcgcagggca tgccgcagcc gcacatccga tccgcggccg cgctccaaga tcacggcgag
     3721 acccaaactg aagaaggcca agccgtatac aaaatatacc aggactaaat aggtgttatc
     3781 gatgccagac acagcactac ctcgatcgga tggggtgcat gaccgccaat tttattgtag
     3841 gccgatcgat ctgtgttgct aagtgctgaa tgtcgcgaat tcgctatgcc gaatgattaa
     3901 aagatgcgca tcttgtcaat tagtagaatc tcagctacac ttcggtgtag aatggaagtt
     3961 ggaaatttag ggttggagat tgcggatgct taacaaaatc tacgcgcatt catgtgaagc
     4021 gatgaccgaa ctcaataacg atagcgtagc cttgacggtg acgtcaccgc cgtactggaa
     4081 cgcgatcgat tacgaggtgc attcgatcga taagcagaag aactatcgaa cgcgcaaata
     4141 tgcgctgggc tacaccgagt atgaagaata tctcacgtgg ctcgatcgaa tcttcagcga
     4201 agtgctgcgt gttacacagc cgggcggctt ctgcgcgatc gtggtcggca ccgtgctgct
     4261 aaacagccgg cactatcccg tgccgttcga tctgatcgca cggctgaccc gatcgggttg
     4321 gctgttccat caagatatca tctggcataa atgcacggcg ggcgtaaagc gcgccggggt
     4381 gacgatccag aaaccctatc cgggctatta ctatcccaac atcatgaccg aatatatttt
     4441 gatcttccgc aagcccggcg atcccatcta caaaactcat tcacttaaag acaagcattc
     4501 agcccaattt ccgatcaatc gagtgttcac catggacacg gccaacaatc tgtggcacat
     4561 cgcgcccgtg ccgcccgatc tgatcgatca tccctgcccg tatccggaag agattcctta
     4621 ccgcttgatt cagatgtatt cgtatccggg cgaagtggtg ctggatccgt ttgttggatc
     4681 ggggcagacg cttaaagttg ccaagtggct cgatcgggct tatgtgggtt atgaaaccat
     4741 tcgaaagtac gtcaatctgg cgaacaaacg cttgaagcaa aaattatcga tcagacccaa
     4801 gcagctgatc gcgcggtttg agaagatcga tttagatgaa ccagccggtc agacgccgac
     4861 gactcgatcg cgtcgcgtga agagttgagc aaacactcat tatcaaaaaa ttggcgaaaa
     4921 acacgaaggc acactgatcg acgcgatcag tgtgccttct cattgcttga ggagtgtcga
     4981 gagatttaga gcgactcgtc tgctacgcca ttgcgagcaa tcacttgctt ataccacagc
     5041 ccgctgtctt tgatgatccg ctgctgcgtc tggtaatcga cgtgcaccag gccgaagcgc
     5101 ttggaataac catagcccca ctcgaaatta tcgagcagcg accaggcaaa gtaaccgcgg
     5161 agattgacgc catcgtcgat ggcgagtttg atttgccgga tgtgttcacg aaaataattc
     5221 aggcggcgcg gatcatggac ctgtccatcc ggactgacct gatcgacaaa ggccgcaccg
     5281 ttctcggtaa tgtacagcgg cggcaagcga taatctcgct tcaattgatt cagcagacgc
     5341 cgcaatcccg gcgcgttaac ttcccagccc atctcggtat gttcaacatt cggcacgttc
     5401 gcaactattt ccccgttctc gaccaagtgc cgcgagtaat agttaatgcc catgaaatcg
     5461 atccgctgcg cgatcaaggc catatcgccg ggctgaatga tcggcacatt cgcaccatag
     5521 gctgcccacg ctcgcgcggg ataactgccg cacacaatgg gctcaataaa ccagccttca
     5581 ttcttcagcc acagttcatc agcctgcgat cgattctcag gagtgtctat ctcgaactca
     5641 tatggccaca tgttgttaac gatacccact tccaacgctg gatcgatcga tcgaatcgcc
     5701 tgcgcggcca aaccatgcgc gaccagtaaa tgatgcgcca cttgcaaagc cagctgctcg
     5761 ttggtaagcc ccggcgcatg ccggccatcc cgatttccga cgaaggccac cacccatggc
     5821 tcgttgaatg ttgcccacga tcgaacccga tcgcccaagc gtcgaacaac cgcagcggcg
     5881 tagtctgcaa aatacgcgct cacttctcga ttgctccagc cgccgcgatc ttgcaaagcc
     5941 tggggcagat cccaatgata aagcgtcgca aagggttgaa tgtctgcgtc gagcagcgca
     6001 tcgaccaatc gatcgtaaaa atccaaaccc agtgggttaa tctggccgaa gccgttgggg
     6061 atcactctcg gccatgagat ggaaaaccgg taagctttca agcccaactg cttcatcaaa
     6121 accaaatcat cgcgccaacg atgataatga tcgcaagcca tatcccccgt gtcaccattc
     6181 tcgacttttc ccaaagtgtg actaaagcga tcccagatcg attcgccttt gccgtcttcg
     6241 ttccaggcgc cttcgatttg ataagaagcc gtcgctgcgc cccagacaaa atccttcgga
     6301 aaaatagggt tcatatcaac tctccagttt ctttaataga gcttgcgcac cagcgagtgt
     6361 ggccgggaca tcgaggccag tgagcaactc ccaagtttcg atgtgagtcg cggtttggcc
     6421 cggctgcaaa ggtcgcaagc cgcccaacgt ttccaactcc atgaagcgat cgttgcaata
     6481 gatttcaaca ttcgaaccaa gatcgggata gagttgatcc gtcgccggct caaaacattt
     6541 acggaacaaa acaccgccaa tcaggtaacc gatccaacca tgtcgattca atgaaccgac
     6601 cttcaaggct ggcagttccg atcgggcctc aatcaacacc ccatcgtcat ctacgtgcaa
     6661 ccgcgagtcg ctccagcgcg tgtaaggcca caaggtcaga tgacgattcg gccgcagctc
     6721 atccgcctcg acagaatcat gtggcagcgg catgatcgcg atcccaccca aagccagctg
     6781 cgtgatcgcc catggcgcac attcgatcga tcgatcgctg tcattagtca attgatggat
     6841 caaagtcacc gcgggtcgat caggcgccaa ctgaatctca atgctcttcc gaatgtcgcc
     6901 cgtcagctgc gcggcccgcg ataaacgcac gccgtttgac aattcttcga tcttcaaacc
     6961 gctgctgtca ggcatatacg tctgcggaaa aatttcgggc gcgcgccaca accgatgtcc
     7021 accgtggaga aaataatcgc cgtggggtgt cgtccatttg aaatcaggca cttcagccaa
     7081 aaaattttgg tcgttgcctg gcaattttaa gcgcacgata cgcggcccag cttcgctcag
     7141 aaattcgagt gacagctgat cgttgcctag gatgtgcgtc ggcaatccat gaaaatcgcc
     7201 cgttactctt tcgaggcctg tcagcacgcg gctcaattta atgtcacctc aaccgaaact
     7261 tcggcttgcc cgtccgctgg caatgaaaca acattacccg cgatcaattc tccattgacc
     7321 cgcagcgaaa cattgttacc tctaccgaca cgtttcactt cgatgcggta agtgacgcca
     7381 cggaaaacgc gcgtcgcgct gaagcctggc catttggtgg gaatcactgg tgcgatcagc
     7441 aagccaccga gcgaaggccg aatgccaaga atccactgcg agatcgccgc gtagttccaa
     7501 gccgcggtcc cggtcagcca cgagttcttc gcttcgccat gcgtgggtgc gtcgcggccc
     7561 gcgatcattt gcgcgtaaac atacggctcg cagcgatgca cctcgctgat ggcttcgcgc
     7621 gccgacggat tgatacgcga gtagtaagca tgcgcttgat cgccgtgccc gataatcgct
     7681 tcggcgatta tgatccacgg attggtgtgg cagaaaattc cagcgttctc tttatacccc
     7741 ggcgggtacg atgaaatttc gccgagattc agataatagt gtgaatatgc tggctgctgc
     7801 aagatgatgc catgctgcgt cgccaaatgt tgattcacgg ccgcgagcgc tcgttcagcg
     7861 cggccatctt ccaggccgat gccgccgatg atgcacatcc cttgcggctc gatgaagatc
     7921 cggccttcgt cacattcatg cgagccgatc ttcgcgccga agttatcgta ggctcgcagg
     7981 aaccagtcgc catcccaacc gtactgatcg acggtggtct tcatcttgac ggcttccggc
     8041 aaacactgct gcgcttcagc tgataagtgg
//
LOCUS       DASRTJ010000510         6937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_165160,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000510 DASRTJ010000000
VERSION     DASRTJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165160"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VFF70_10290"
     CDS             <1..1602
                     /locus_tag="VFF70_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZY45126.1"
                     /translation="GVAAARRSNPESWTDGLTMIQPEKEGPQPWASVHETFPTTVAEI
                     YNVDNVGPHGSLLSGWEMFRRAGEDLDVLIRQAHVARKRILPIGAGWALSKINITDGW
                     LVNTKLLNGCYDVSDKYFDAAYPAERRPNLVLAQAGMQIAELNTYLELLPRIEGNRRA
                     LQAAGIGNGQTVAGSISGNTHGSQLTYGAMPDFVTGLHIATGSGQTIWLERQSKPVMN
                     QDFVQKIGARLIRDDDIFNAAVVSFGTFGIIAALAVETAPIYQLEFPPIANISYAKLK
                     EKLNGFAAGIIPQDLYHYEFIFDPFSPAHIAMEASAPKVPYIPGVPTPKPRWIIRDKK
                     GYAPGINILRVLGPFRNLLPPRFLTGIEYKQYRKNALLNKVRGSPGQLYTSSIYYLGG
                     YIESAFAVSVKDAAATIDISSQVAQELSLPSIYQVRLCRASTGTLAFTQHEPITAVFE
                     LGMINDERFPEFEQRLDEAFRKAGIRYTMHWSKNSGISPDKLKYMYGDAKIKSWKAAR
                     SAVFGGDATLMKLFETDAMVEAGLA"
     gene            complement(1960..3018)
                     /locus_tag="VFF70_10295"
     CDS             complement(1960..3018)
                     /locus_tag="VFF70_10295"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZY45127.1"
                     /translation="MTFNFHSPTSNFQQPNGQGDNLLEVESVVKYFPVRGGILQRTVA
                     QVQAVENVSFTVKRGETLGLVGESGCGKTTIGRMIVRLTEPTSGAIRFEGQDILPLKG
                     NNLKRLRREMQIIFQDPYSSLDPRTPVGDSIGEGLLVHGVKSAHERQRQVLEILQKVG
                     MEDYHAKRYPHEFSGGQRQRIGIARALILRPKFLVCDEPVSALDVSIQSQVLNLLREL
                     QHEFGLTYIFIAHNMSVVEHISDRVAVMYLGKLVELADRIELYRNPQHPYTQALMSAI
                     PVPNPKLRRERTILKGDVPSPLNPPTGCRFHPRCPVAIDICSKVEPPLLDLGGGHLAA
                     CHLRQPASGAGISKPASA"
     gene            complement(3123..4109)
                     /locus_tag="VFF70_10300"
     CDS             complement(3123..4109)
                     /locus_tag="VFF70_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45128.1"
                     /translation="MASQPDTLLEIRDLHTNFYTHDGVVRAVDGLNLYVKRGEVLGLV
                     GESGCGKSVTSLSVLRLVSPPGKIDSGEILFDGKDLLKFSGHEMTQIRGNRIAMIFQQ
                     PTSSLNPVFRIGDQISEVLNIHQDLGKKAGKARALELLSMVGIPDPERRLNAYPHELS
                     GGMAQRVMIAMALACVPELLIADEPTTALDVTIQAQILDLMRDMRSKLDTAIVLITHD
                     LGVVAEMADRIAVMYAGQIVEEAPVDELFANPKHPYTRGLIGSIPVLGVVKDALDVIP
                     GNVPNLIDLPVGCRFAPRCRARVENNLQICLEKVPELKPVAVNHTARCWLYE"
     gene            complement(4147..5100)
                     /locus_tag="VFF70_10305"
     CDS             complement(4147..5100)
                     /locus_tag="VFF70_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45129.1"
                     /translation="MQSADAVPNAVAAKVEAPSSLFREILRRLLRNRSAQVGLVILTL
                     LLLVALFAPAIAAHDPIDFTDPSNTTRTPPCVHLFGCPADQPEYIFGLDGNGRDLFSR
                     VVFATQVSLFIGISTVTFAIVIGTLIGALAGYAGGWFENIAMRFMDVLLAFPSLLLAI
                     ALVAILRPIILEGGISPLIPALFAIGFVSIPVYARIVRASVISIKEQDFVAADRALGV
                     SPLRLLFHRILPNALTPLIVQGTLGIATGILDAAALGFLGLGQQPPFPEWGTMLGVER
                     NSVFNAPHLLIFPGLAIMITVLSFNLLGDGLRDALDPRLYR"
     gene            complement(5106..6182)
                     /locus_tag="VFF70_10310"
     CDS             complement(5106..6182)
                     /locus_tag="VFF70_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45130.1"
                     /translation="MIQFLVRRLLLIIPVLIGIIFVTFVMVHAIPGDPCRAMLGERAT
                     DRTCNAFKERFGLNDNVVVQFGRYIGNLVQGDLGTSIKYGRPVGDILIERLPMTLEIS
                     IGAMLFAILLGIPLGIISAYRHNTPIDAVTMVGANIGVSMPIFWLGLLLAALFGVALK
                     GTFLQLPTSSRLTAGLTIVPLAESWHLQNLSGVGKTLITFLSNTFTFNALVTGNWKVY
                     GDAIKHLILPSIALGTIPLAIMARITRSNLLDIMGLDYIRTARAKGLTERVVLFKHAM
                     RNAMLPLVTIIGLQFGGLLGGAVLTETVFGLPGMGSFLVDSIFARDYPIVQGFTLVIA
                     IIFVFVNLLVDISYAYLDPRVRLS"
     gene            complement(6458..>6937)
                     /locus_tag="VFF70_10315"
     CDS             complement(6458..>6937)
                     /locus_tag="VFF70_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY45131.1"
                     /translation="PMVPVAHGGSAAAFKATVAGAYSDPLAGEQFRLMSIPGQDQIVW
                     SQNAEPISLYCADETDGETLRACEQVFDALLSYEPGGTAVKPGLATGCTSNADLTVWT
                     CTLRAGAKFSDGSAVTANDVVKTYGVQWDAADPLHTGSTGNFDYWSAFFGGFLNPPK"
BASE COUNT         1650 a         2103 c         1851 g         1333 t
ORIGIN      
        1 ggcgtggctg ccgcccgccg atccaatccg gaatcctgga ctgacggcct gacaatgatc
       61 caacccgaaa aagaaggccc acagccgtgg gccagcgtcc acgagacatt cccgacaacg
      121 gtggccgaaa tctacaacgt cgacaacgtc ggtccccacg ggagcctgct ctcggggtgg
      181 gagatgttcc gacgcgccgg cgaggacctc gacgtcctga tccgccaggc ccacgtagcc
      241 cgcaagcgca tcctcccgat cggcgcggga tgggcgctgt cgaagatcaa catcaccgat
      301 ggctggctgg tgaacaccaa gctactgaac ggctgttacg atgtcagcga caagtacttc
      361 gatgccgcgt atccggcgga gcggcgtccc aacctcgtcc tcgcccaggc cggcatgcag
      421 atcgccgagc tcaacaccta cctcgagctc ctcccgcgga ttgagggcaa ccggcgggcg
      481 ctccaggccg cggggatcgg caacggccag accgtcgcgg gatccatctc cggcaacacc
      541 cacggatcgc agctcaccta cggggcaatg cccgacttcg tcaccggtct gcacatcgcg
      601 accggcagcg gccagacgat ctggctcgag cgacagtcga agccggtcat gaaccaggac
      661 ttcgtccaga agatcggcgc ccggctgatc cgcgacgacg atatcttcaa cgcggccgtg
      721 gtcagtttcg gcacattcgg gatcatcgcc gccctcgcgg tggagactgc tccgatctat
      781 cagctcgagt tcccgcccat cgccaacatc agctacgcca agctgaagga gaagctcaac
      841 ggcttcgctg ccggcatcat tccgcaagac ctctatcatt acgagttcat cttcgacccc
      901 ttctcccctg cgcatattgc catggaggca agcgcgccga aggtgccata catacccggc
      961 gtaccgacgc ccaaaccccg ctggattatc cgcgacaaga aaggctacgc gcccggcatc
     1021 aacatcctgc gagttctcgg ccccttccgc aacctgttgc cgccgcgttt cctgacggga
     1081 attgagtaca agcagtaccg caagaacgcc ctgctgaata aggtgcgcgg cagtccgggc
     1141 cagctctaca cctcgtcgat ctactacctc gggggctaca tcgagagcgc attcgcggtg
     1201 tcggtcaaag acgcggcggc gacgatcgac atctcatcgc aggtggccca ggaactgagc
     1261 ctgccctcga tctaccaggt gcggctctgc cgcgcctcga cggggacgct cgcgttcacc
     1321 cagcacgagc cgatcaccgc ggtcttcgag ctcgggatga tcaacgacga gcgcttccct
     1381 gagttcgagc agcgccttga cgaggcgttt cggaaggcag gcatccgcta cacgatgcac
     1441 tggtccaaga attccgggat cagtccagac aagctcaagt acatgtacgg cgatgccaag
     1501 atcaagagtt ggaaggccgc gagatcggcg gtcttcggcg gtgacgcgac gttgatgaag
     1561 ttgtttgaaa ccgatgcaat ggtcgaagcg gggttggcgt gacctcccga cctacttgtg
     1621 ctgaaccgga gcgagatcgc aaggtatctc tcgctcgcag cacctgtccg tcggttgcca
     1681 gccagagtcg ttgtggagat tgtggtacac cgtgacggca cccctattaa agttcggctg
     1741 gggcgcactt cgggagaacc ggatccccag gctggatctc cttgccctac atcgcatagc
     1801 ccggatccaa gaggccgcct aaccagcaaa tgaagctgcg gggcgcgcgg ctcccgccgc
     1861 gcgccccgca gcttatttgc gattcgttag gcacgaccga tacgaaataa aactcctcaa
     1921 agccgaaaac tctgaggagt tttttgatga atcgagttct tacgcactcg ccggcttact
     1981 aattccggca ccactcgccg gctggcgcaa gtggcacgcc gccaaatgcc caccaccgag
     2041 atcgagcagc ggcggttcga ctttggagca aatatcgatc gccaccgggc agcgcggatg
     2101 gaagcggcag ccggtcggtg gattcaacgg gctcggcaca tcgcctttga gaatcgtgcg
     2161 ctcgcggcgc agtttcggat tcggcaccgg aatggccgac atcaacgcct gcgtgtatgg
     2221 atgctgtgga tttcgataca gctcaatccg atcggccaac tcgacgagct tgcccagata
     2281 catcaccgcc actcgatcgc tgatgtgctc gaccacgctc atgttatgcg cgatgaagat
     2341 atacgttagt ccgaattcat gttgtagctc gcgcagcaga ttcaacactt gtgattggat
     2401 cgacacatcg agcgcagaca ccggctcatc gcacactaaa aatttggggc gcagaatcaa
     2461 cgcgcgggca atgccgatgc gctgacgttg gccgccggag aattcgtgtg gatagcgctt
     2521 ggcatgataa tcttccatgc ccactttttg tagaatctcc aacacttggc gctggcgctc
     2581 gtgtgcgctc ttcaccccgt gcaccagcag cccctcgccg atgctatcgc ccaccggcgt
     2641 gcgcggatcg agcgacgagt acgggtcttg aaaaataatc tgcatctccc ggcgcagccg
     2701 cttgagatta ttgcccttca acggcagaat atcctgcccc tcaaaacgga tggcaccgct
     2761 ggttggttca gtcagccgga caatcatgcg gccgatggtc gttttgccgc aaccgctttc
     2821 gccgaccagc cccaaagtct caccacgctt caccgtgaag ctgacattct caaccgcttg
     2881 cacctgcgca actgtgcgct gcagaattcc gccgcgtacc ggaaaatatt tgacgaccga
     2941 ctcgacttcg agcaagttat cgccctgccc gttgggttgt tggaagtttg aggttggaga
     3001 atggaagttg aaagtcattg ccttgttgct gatccgctcc cttcgactgc gatgtgacta
     3061 ctgtaaattc acatctccgc tcaggatgct ttcactaatt acttcaccct aatcatcccg
     3121 cattattcat acagccagca gcgcgccgtg tgattaacgg ccaccggctt caattcagga
     3181 actttttcca ggcaaatctg taaattgttt tcgacgcggg cgcggcagcg tggcgcaaac
     3241 cggcagccta ccggcagatc gatcagattc ggcacattgc cgggaatcac atccaacgcg
     3301 tctttcacta cgcccagcac cgggatcgat ccaatcaagc cgcgcgtgta tggatgtttt
     3361 gggttggcga acaactcatc gacgggcgct tcttcaacga tctgtccggc gtacatcacc
     3421 gcaatccgat cggccatctc ggccaccacg cccagatcgt gcgtgatcaa aacgatggct
     3481 gtatccaact tcgagcgcat atcacgcatc agatcgagaa tctgtgcttg aatggtcacg
     3541 tcgagcgcgg tcgtcggctc gtcggcgatc agcagttccg gcacgcaggc caacgccatg
     3601 gcgatcatca cgcgctgagc cataccgcct gagagttcat gcggatacgc attcaagcgc
     3661 cgctccggat ccggaatgcc caccatcgat agcagctcca gcgcgcgcgc cttacccgct
     3721 ttcttgccca aatcctggtg aatattcagc acttcggaaa tttgatcacc aatcctaaag
     3781 actggattca acgaggaagt cggctgttga aagatcatcg caatacgatt gccgcgaatc
     3841 tgagtcatct cgtggccgct aaacttcaac aagtctttcc cgtcgaacaa gatttcgccc
     3901 gaatcaatct tgcccggcgg cgagaccaag cgcaggacag acagtgaagt cacgctcttg
     3961 ccgcaaccgg actcaccgac caagcccaag acttcgccgc gcttgacgta cagatttaac
     4021 ccatcgaccg cccgcacaac gccatcatgc gtatagaagt tggtatgcag gtcacgaatt
     4081 tctaacagtg tatccggctg tgacgccatg taactctatc tccacatcag tatcaacaac
     4141 tcaaccctaa cgatacaagc gtggatctaa cgcatcgcgc aagccatcgc ccagcaagtt
     4201 gaaagacagc accgtgatca tgatcgccag gccggggaag atcaacaaat gcggcgcgtt
     4261 gaagacgctg ttgcgctcga cgcccagcat cgtgccccat tccggaaacg gcggctgttg
     4321 tcccaatcct aagaaaccca aggccgcggc atccaatatt cctgtcgcaa tgcctaaggt
     4381 gccctgcaca atcaacggcg tcagtgcatt cggcaagatg cgatggaaca acaagcggag
     4441 cggtgaaacg cccaacgcgc gatcggccgc cacgaagtcc tgctccttga tcgatatcac
     4501 gctggcccgc acgatccgcg cataaaccgg gatcgataca aagccgatcg caaacaacgc
     4561 tggaatcaac ggtgatattc cgccctccag aatgatcggc cgcaggatgg cgaccaaagc
     4621 aatcgccaaa agcaagctcg gaaaagcgag cagcacatcc atgaagcgca tggcaatatt
     4681 ttcgaaccag ccgcctgcat agccggccag cgcaccgatc aacgtgccga tcacaatcgc
     4741 aaaggtaacg gtcgagatgc caataaacag tgagacctgt gtagcaaaca ccacgcggct
     4801 gaacagatca cgcccgttgc cgtccagccc gaaaatatat tcaggctgat cggccgggca
     4861 gccgaagaga tgaacgcacg gcggcgtgcg agttgtatta ctgggatcag taaaatcgat
     4921 cgggtcgtgc gctgcaatcg ccggcgcaaa gagcgcgacc aacaataaca gggtgaggat
     4981 caccaatccg acttgagcgg atcgattgcg caggagtcgt cgtaggattt cccgaaacag
     5041 actgctcggt gcttcgactt tggcggctac ggcgttcggc acggcatcag cagattgcat
     5101 aaggattaac tcagccgcac gcgtggatca aggtaagcgt acgaaatatc cactaagaga
     5161 ttgacgaaca caaagatgat ggcaatgacc aacgtaaagc cttggacgat gggatagtca
     5221 cgcgcaaaga tcgaatcgac gagaaagctg cccatgcccg gcagaccaaa gacggtttca
     5281 gttaagaccg cgccgcccag caagccgcca aactgcaggc cgatgatcgt gactaagggc
     5341 aacatcgcgt tacgcatcgc gtgcttgaac agcactaccc gctcggtcaa ccccttggcg
     5401 cgcgccgtgc gaatgtagtc cagacccatg atatccaaca gattggagcg ggtgatgcgc
     5461 gccatgatcg ccagcggaat agtgccgagc gcaatcgaag gcagaatgag gtgtttaatc
     5521 gcatcgccat agactttcca attaccagtg accaatgcat taaacgtgaa ggtgttggac
     5581 aaaaatgtaa tcagcgtttt gccgacaccc gacaggtttt gcaaatgcca actttcagcc
     5641 aacggcacta ttgtcaggcc ggctgtcaag cgactagagg tgggtaattg caagaaagtc
     5701 ccttttagcg ccacgccaaa caacgccgcc agcaacaatc ccagccaaaa aatgggcatt
     5761 gagacgccga tgtttgcgcc aaccatggtc actgcatcga tcggcgtgtt gtgccgatag
     5821 gcggaaataa taccgagcgg aatgcccagg aggatcgcga acaacatcgc cccgatcgat
     5881 atttcaagcg tcatcggtaa acgctcgatc agaatatcgc caaccggacg accatacttg
     5941 atcgacgtac ccaaatcgcc ttgcactaaa ttgccgatgt accgaccaaa ctgaacaacg
     6001 acattgtcat tcaggccaaa ccgttccttg aaagcgttgc aggttcgatc ggtagcgcgc
     6061 tcgcccagca tcgcacggca gggatcgccc gggatggcat gcaccatcac gaacgtcaca
     6121 aaaataattc caatgaggac gggaatgatc aacagcaaac gacgaactaa gaattggatc
     6181 atcgcttatc gcttttgctc agtcgcagat aaatagcata caccgaatat gttaatcagt
     6241 aataggtctt tcgcttcagg aatcttttta aggttgtcgc cctgagcgca gcgaagggtc
     6301 tcgtgcaatt ttccgagatg cttcgctgcg ctcagcatga caggcgaaaa ttctaagtaa
     6361 gatcaaaatt tatcaaacct tagcacgatg ggtggagcgc ccgtgagcac cccacccatc
     6421 ctgcatcttc aaaacaattt acttcgcagt caaccaatta cttcggcggg tttaagaatc
     6481 caccgaagaa cgctgaccag taatcgaagt taccggtgct gccggtatgc agcggatccg
     6541 ctgcatccca ttgcacacca taggtcttaa cgacatcgtt cgccgtcacg gcagagccat
     6601 ctgagaactt agcgcctgcg cgcagcgtgc aagtccagac cgttagatcg gcattagacg
     6661 tgcagccagt ggccaggccg ggtttcactg cagtgccgcc cggctcgtag gacagcaacg
     6721 catcgaagac ctgctcacaa gcacggagtg tttcaccatc ggtttcatcc gcgcaataca
     6781 ggctgatcgg ctcagcattc tgactccaga cgatttgatc ttgtccgggg atgctcatca
     6841 agcggaattg ctcgcccgct agcgggtcag agtaagcacc ggctacggtt gccttgaagg
     6901 cagccgcaga accgccgtgc gccaccggga ccatcgg
//
LOCUS       DASRTJ010000511         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_127045,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000511 DASRTJ010000000
VERSION     DASRTJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127045"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFF70_10320"
     CDS             <1..403
                     /locus_tag="VFF70_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45132.1"
                     /translation="LPQNFALQATASYTGTNDNGYGVIFRVVADGKFYVFRISGDGFY
                     TVERADVDKLTTLIDWMQSDLIRQDPRVANVLTVHGQGGHYTLYINDQQVDQFDDQTY
                     ADGSYGVIVDNFDDQSSVQLLFDVYTVGTPK"
     gene            470..1963
                     /locus_tag="VFF70_10325"
     CDS             470..1963
                     /locus_tag="VFF70_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45133.1"
                     /translation="MHTTEATKTLLPNNRGILAALIAIVLLGAALRFTGLDHESLWND
                     ELERYRQANQVDLLHAIKAGWTYEPHPPGYRVFLYFSEKIWGTSEFALRFPGAVAGIL
                     AIIAIFLLGRKLYSEREGLIAALFMAVQWCPIYYSQDAGPYPILMLLSIVTVLVWRPL
                     VEQLRQGRNPPRALSLTYILVSIVTCWLHYFGLFFIALQGLATIWIVRSAQQKLKPGA
                     RIFAPIFIAFLPWVPFALRSLGRGPIWIPYPGEPISAAADYFAFFFNQSIGLVALVAL
                     IALWVMLRRAVRMFRQREHLHINLLSPDQLLVAWAAIPFTIVYLISLRTPILTLRNMI
                     IALPAVYLLSARSITRLTPKPIGQTAISVALAAVFMYNLLYVDNYYTQPHKDQFREAV
                     ASIVQRADSYPQAYILGYGYGSEYFDYYFQHFGSPLRVDLSTYDSNQFPIVTAAIKRA
                     QPKYVWLVSGLIIPNDLLLNYFNQNYQLIDQQPFFQAGVWLYQKKTS"
     gene            1977..2378
                     /locus_tag="VFF70_10330"
     CDS             1977..2378
                     /locus_tag="VFF70_10330"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZY45134.1"
                     /translation="MSVSTSATFMLRPDVVATVLEDGAVLLDLETKYFYSVNQSGWAI
                     LQLFEIGATRPQVVDQCRAWGVVAPEADGVLKFLDTLLSENLITALQQPSSAPSIEWT
                     GMWTTPTIEKHREPLQRIMISAFDPTLPLAE"
     gene            2388..3353
                     /locus_tag="VFF70_10335"
     CDS             2388..3353
                     /locus_tag="VFF70_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45135.1"
                     /translation="MSSEIFYCGPLAVSLTAQTQALRAKVVEALDLYTVTWPPPLFPI
                     SIDIRERDSFASMPAGTYMKCSRMNVDPVAGGLRAAVPSGAECIYLSANSLWSISVPL
                     NGQHVPEDVEDLIGLVLTNSWRQAKWVPIHAGAIVNGSICAILCAPSGGGKTTVTASM
                     IRRGWQTLGDDKILLRLQSNGSPLLAALLHNFNLHPKTRDWFPEVGDLTHLPRYSAWT
                     EKRKVRIVDIWPGSEVLHAVPTHLIQLERSEQPKAVELTKLTPDEVLSIFLRQTVIPK
                     DRSFAQLILSTVASTAQRLTGWRVEVGDQAYHDPDCLLPLERVLQ"
     gene            3350..4267
                     /locus_tag="VFF70_10340"
     CDS             3350..4267
                     /locus_tag="VFF70_10340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45136.1"
                     /translation="MSELSVSVVIPVYNAEDTIGNLLSALLNQSGISTQTEYIIVDNG
                     STDRTIEIVQKFPVTLLRESTPGPAAARNCGLFAATSDVIAHLDADTQPTRRWLAEIV
                     APFNDPMVMLVAGKTLAFVPQNGVERYFAQVHLFDAEANIKRPIFPFAASMNVAVRRS
                     AAIEIGGWDESMLTTEDVDFCYRILQKFSEPIHYAPNALLLHRNRSTLEDMQRQAWTY
                     GEGSGKLYAKYPEMLHWGWPQAFNAQRNLFMYGLWPHIIRVTKWLGLASSDAVEFAHY
                     HHLWYSAFWAGFYNFRRSGCFMPTPNKGL"
     gene            4264..4746
                     /locus_tag="VFF70_10345"
                     /pseudo
     CDS             4264..4746
                     /locus_tag="VFF70_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
     gene            4757..>5469
                     /locus_tag="VFF70_10350"
     CDS             4757..>5469
                     /locus_tag="VFF70_10350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45137.1"
                     /translation="MLRVSIVVPTFNSAQTIAECLQAIGSQSLPRDQYEVILVDNGSR
                     DATTTIADQFDAKQLLQNTRGAAQARNVGWAAARGEWIAFTDSDCIPSRMWLQSLLDR
                     AATDRMAAHPIGVAGRTLGYRSTSAAARYVDLTGGLDAETYLAHPAFPWAPTNNVMYL
                     RSALETVEGFDARYVTYEGGDLSYRLQTVFNHALVFEPRAVVLHQHRSTWRAYWRQQY
                     GYGRGYGQFIWKHRDHFRWT"
BASE COUNT         1178 a         1568 c         1456 g         1267 t
ORIGIN      
        1 gctgccgcag aactttgcgc tgcaagcgac agccagttac accggcacaa atgacaacgg
       61 ctatggtgtg atcttccgcg tcgtggcgga cggcaagttc tacgtcttcc gcatttccgg
      121 cgacggcttc tataccgtcg aacgcgcgga tgtcgataag ctcacgacgt tgatcgattg
      181 gatgcaatca gatctcatcc gccaagatcc gcgggtcgcc aacgtgctga cggtgcacgg
      241 tcagggcggc cactacacgt tgtacatcaa cgaccagcag gtcgatcagt ttgacgacca
      301 gacctacgcc gacggatcgt acggcgtcat cgtcgataac ttcgacgatc aatcttcggt
      361 tcagttactc ttcgacgttt acaccgtcgg cacgccaaag tagatgcact aaaacggagt
      421 ggacttgagc acagccatgt tcaagtccac tcgagatctt cattaaaaca tgcatacaac
      481 tgaagcaaca aaaactctcc tccccaataa tcgaggtatc cttgccgcgc tgatcgccat
      541 cgtcctactg ggcgcggcgc tgcgcttcac cggcctcgat cacgaatcac tgtggaatga
      601 tgagctagaa cgttaccggc aggcgaatca ggtcgatcta cttcacgcga tcaaagccgg
      661 ctggacatat gaaccacatc cgccgggtta ccgtgtgttc ctgtacttct ccgaaaaaat
      721 ttggggaacc tctgaatttg cgctgcgctt ccccggcgca gtggctggca tactggcaat
      781 catcgccatc ttcctgctgg gccgcaagtt gtactccgag cgcgaaggct tgatcgccgc
      841 gctgttcatg gccgtccagt ggtgcccgat ctattacagt caggatgccg ggccttatcc
      901 gatcttgatg ctgttgagca ttgtgaccgt tctcgtgtgg cgaccattag tcgagcagct
      961 gcgccaaggc cgcaacccgc cgcgcgcatt gtcactgacc tatattttgg taagcatcgt
     1021 cacgtgctgg ctgcattact tcggtttatt ctttatcgcg ctgcaaggac ttgccacaat
     1081 ttggatcgtg cgatccgcgc agcagaaatt gaaaccgggc gcgcgcatct tcgcgccgat
     1141 ctttatcgct ttcctgccct gggtgccttt cgctctgcgc tcgttgggtc gcggcccgat
     1201 ctggattccg tatcccggcg aacctatcag cgctgcagcg gactacttcg ctttcttctt
     1261 caaccaatcc attgggttgg tcgcgttagt tgcgctgata gccttatggg tcatgctgcg
     1321 ccgcgccgtt cgaatgttca gacagcgcga acacctccac atcaatctgc tgtcccctga
     1381 tcaactgctg gtggcctggg cagcgattcc atttacaatc gtttacctga tctcactacg
     1441 aacaccgatc ttgacgctgc gcaacatgat catcgccctg cccgccgtct atctgctctc
     1501 cgcccgatcg atcacgcgct tgacgccaaa gccgattggg caaacggcca tcagcgtcgc
     1561 gctggctgcg gtgttcatgt ataatctgct ctacgtcgac aactactaca cgcagccgca
     1621 caaagatcaa tttcgggaag cggttgccag cattgtgcag cgcgccgaca gctatccgca
     1681 ggcctacatc ctgggctacg gctatggcag cgaatacttc gattattatt ttcagcattt
     1741 tggttcgccg ctgcgcgtcg acctgagcac ttatgacagc aatcaatttc caatcgtcac
     1801 agccgcgatc aagcgtgccc agccgaaata tgtctggctg gtcagcggtt tgatcatacc
     1861 gaatgatcta ttgttgaatt atttcaatca aaattatcag ctcatcgatc aacagccatt
     1921 cttccaggcg ggtgtctggc tgtaccagaa aaaaactagt taggagataa atttagatgt
     1981 ctgtttcaac ctctgccact ttcatgctgc gacccgacgt ggtcgccacc gttttggaag
     2041 acggcgccgt gttattggat ctggaaacta aatatttcta ttcggtcaat caatccggct
     2101 gggctatctt gcaattgttt gagatcggcg cgacacggcc ccaggttgtg gatcaatgcc
     2161 gggcgtgggg tgtggttgcg ccggaagccg atggcgtcct gaagttcttg gatacactcc
     2221 tcagtgaaaa tctgattacc gccttacagc agccctcgag cgcgccgtca attgaatgga
     2281 ccggcatgtg gaccacgccg acaatcgaga agcatcgcga gccattgcag cgcatcatga
     2341 tcagcgcctt cgatcccacg ctgccgctgg cagaataact gcgcagtatg agttcagaaa
     2401 ttttttattg cggaccgctg gccgtcagcc tgacggcaca gacccaagca ctgcgcgcta
     2461 aagttgtcga agcacttgat ctgtacacgg tgacctggcc gcctccgctc ttccccatct
     2521 cgatcgatat tcgtgagcgt gattcgtttg cgtcaatgcc agctggaacg tacatgaaat
     2581 gctcgcgcat gaatgtcgat ccggtggcag gcggcttgcg ggcagccgtg ccgtccggcg
     2641 cagaatgcat ctacctctca gccaatagcc tttggtcaat cagcgtacca ctgaatggcc
     2701 agcacgtgcc agaagatgtt gaagacctga tcggcctggt gttgaccaac agttggcggc
     2761 aagccaagtg ggtgccgatt cacgccggag cgatcgtcaa cggatcgatc tgcgcaatct
     2821 tgtgcgcgcc atcgggcggc ggcaaaacaa ccgtgaccgc ttcgatgatc cgtcgcggct
     2881 ggcagacttt aggcgacgac aaaattttgt tgcgactgca gtccaatggt tcgcccctac
     2941 tcgccgcctt gctgcacaac ttcaatctgc atcccaaaac ccgcgactgg tttcctgaag
     3001 tcggcgacct tacacatttg cctcgctatt cggcctggac tgaaaaacgc aaagtgcgca
     3061 tcgtcgacat ttggccgggc agcgaagtcc tgcacgccgt gcccacgcat ttgattcaac
     3121 tcgagcgcag tgagcaacca aaggccgttg agctaacgaa acttacgccg gacgaagtct
     3181 tatcgatctt tttgcgacag acagtcattc ccaaagatcg atcgttcgcg cagctgatct
     3241 tatcgactgt cgccagcacg gcgcagcgct tgaccggttg gcgcgtcgaa gtgggtgatc
     3301 aagcctatca tgatcccgat tgtttactgc cgcttgaaag agttttgcaa tgagtgagct
     3361 atccgtcagc gtggtcatac ccgtttacaa cgccgaagat acgatcggca atctgctatc
     3421 ggcgctgctg aatcaatcgg gcatatcgac acaaaccgaa tatatcatcg tcgacaatgg
     3481 ctcgaccgat cgcaccatcg agatcgttca aaaatttcca gtgacgctgc tgcgcgaatc
     3541 aacccccggg ccagccgccg cacgcaactg cggtttattt gcggcgacca gcgatgtgat
     3601 cgcgcatctg gatgcggaca ctcaacccac gcggcgctgg ctggcagaaa ttgtcgcgcc
     3661 cttcaacgat ccgatggtga tgttggtggc gggcaagacg ctggcgtttg taccacagaa
     3721 cggcgtcgaa cgctattttg cacaagtgca tttatttgac gccgaagcaa acatcaagcg
     3781 cccgatcttt ccgtttgccg catccatgaa cgtagccgtg cggcgatcgg cggcgatcga
     3841 gatcggcggc tgggatgaat cgatgctcac gacggaagac gtggattttt gttatcgcat
     3901 tctgcaaaaa ttttccgagc cgattcatta cgcaccgaac gcgctgctgc tccaccgcaa
     3961 tcgatcaacg cttgaagaca tgcagcggca agcgtggacc tacggcgaag gttctggcaa
     4021 actttacgcg aagtatcctg agatgttgca ctggggctgg ccgcaagcgt ttaacgcgca
     4081 gcgcaatttg ttcatgtacg gcctttggcc gcacatcatt cgcgtgacca aatggctggg
     4141 tctggcttca agcgatgcgg tcgagttcgc acactatcat catttgtggt acagcgcgtt
     4201 ttgggcggga ttttataact tccggcggtc tggctgcttc atgcccactc cgaacaaagg
     4261 cttatgacga tatccgtctc agtgatcatt ccggtctatc ggcgtgacgc cgaactaacc
     4321 ccttgcttgc aggcccttgc cgcccaaagt ctgccgcgcg atcaatacga agtcatcatc
     4381 gtggatcgca gcaccgatca atcaacgcag ttgatcgcag aataattcaa cacgcggcac
     4441 attcagcaga ccggccgtgg catgtccgat gcgcgcaatg tcgggctgac cgccgcgcag
     4501 ggaaaatgga ttgcctttac ggacgcagac tgtattccat ctagacggtg gctcaaattt
     4561 ttatctgaag cggtgagcca cgaaaacatg tggggcgcgg ccggcaaaac cgtgggtcac
     4621 gcctcgcgat caaacgcggc acgtttcgtc gatctagccg gcaggctgaa tgtcgaaaaa
     4681 tatttggcgc aatatttagg ctttatcaac acttattaca atcgcgctga gcacgcgcgc
     4741 gggtaatcga ttatctatgc ttcgagtctc aatcgtcgta cctactttca acagcgcgca
     4801 gacgatcgcc gaatgcttgc aggccatcgg ctcgcaaagt ttgccgcgcg atcagtacga
     4861 agtgatcctc gtcgacaacg gatcgcgcga tgcgacgacg acaatcgccg atcaattcga
     4921 tgcgaagcag ctactgcaaa acacgcgtgg tgcggcccaa gcgcgcaatg taggttgggc
     4981 ggccgcgcgc ggtgaatgga tcgccttcac cgattcggat tgcattccct cgcgcatgtg
     5041 gctgcaatcc ttattggatc gcgcagcaac tgatcgaatg gctgcccacc cgatcggggt
     5101 ggcgggtcgc acgctcggtt atcgatcgac atcggcggca gcacgctatg tcgatctcac
     5161 cggcggtctg gatgctgaga cttatttggc tcatcctgcc tttccgtggg cgccgacgaa
     5221 caacgtcatg tatctccgat cagctttaga aaccgtcgaa ggctttgatg cgcgctatgt
     5281 gacttacgaa ggcggcgatt tgagctatcg cttgcagact gtgttcaatc atgcgctggt
     5341 ctttgagccg cgcgcggtgg tgctgcatca acatcgatcg acctggcgcg cctattggcg
     5401 gcagcaatac ggttatggcc ggggttatgg ccaattcatc tggaaacatc gcgatcactt
     5461 tcgctggac
//
LOCUS       DASRTJ010000512         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_127170,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000512 DASRTJ010000000
VERSION     DASRTJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127170"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFF70_10355"
     CDS             <1..395
                     /locus_tag="VFF70_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45138.1"
                     /translation="TGPTPFDAAVNVLIAEARRLAHLFITEHLMTIELSHGRRLRLGE
                     DLSAEFPPSLKTITHADLRALLDRIDPTPDSLRESGVVDWADLSDRIHFIADMFRCYQ
                     EYPALFEPPFKPDQVTSLKAGQLPGGRR"
     gene            complement(476..940)
                     /locus_tag="VFF70_10360"
     CDS             complement(476..940)
                     /locus_tag="VFF70_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZY45139.1"
                     /translation="MSRNTLVVRHDPFFMPLRDAMDTLFNDSFISPVARNWATVWGIG
                     SRLPINIYADADGYTFVAVVPGLKADDVTIEVEGNTLKLSGETIAPAISADEKVRALR
                     SEIGFGKFNRSFELPDAIDATQIDANLENGLLTVRVAKAETVKPRSIKVQAK"
BASE COUNT          375 a          431 c          399 g          360 t
ORIGIN      
        1 tcacaggccc caccccgttc gatgcggccg tcaacgtcct catcgccgaa gcgcgccggc
       61 tggcacatct cttcatcacc gagcacctga tgacgatcga attgtcgcac ggcaggcggc
      121 tgcggctggg cgaggatctc agcgccgagt ttccgccgtc gctcaagacg atcactcacg
      181 ctgatttgcg cgctttgctc gatcggatcg atccaacgcc ggacagcctg cgcgaaagcg
      241 gtgtagtcga ttgggctgat ctgtctgatc ggatccactt catcgcggat atgttccgct
      301 gctatcaaga atatccggcg ttgttcgagc cacccttcaa gcccgatcag gtcacgtcac
      361 tgaaggccgg ccaacttccc ggtggtcgac ggtaagcgtt cgatacgcaa caaaaagagc
      421 cgccccaacc tggggcggct ctccaacttc taacttccaa catccaattt ccaaattact
      481 tggcctgaac cttgatcgat cgcggcttga cggtttcagc cttggccacg cgtacggtca
      541 agagaccgtt ctccaaattc gcatcgatct gggtcgcatc gatcgcgtcc ggcagttcga
      601 acgatcgatt gaatttgccg aaaccgatct cgctgcgcag cgcgcgaact ttctcatcgg
      661 cgctgatcgc cggagcgatc gtttcaccgc tgagcttgag cgtgttgccc tccacctcga
      721 tcgtgacatc gtcggccttc aggcccggca cgacggcgac gaaggtgtag ccgtccgcgt
      781 cggcgtaaat gttgatcggc agacgtgagc cgatgcccca gacagtggcc caattgcgcg
      841 cgacggggct gatgaacgaa tcattgaaca gcgtatccat cgcatcgcgc aagggcatga
      901 agaacgggtc gtgtcgaacg actaaagtat tgcgtgacat ttctattgcc tcccagtaag
      961 aatttatgat tttcgatttt tgatctctga ttgctcagaa ctgtgaatag catattcgat
     1021 gtgcgttaga tttccgcaag cgaagtgtta gcgatgtgtt agagttgcaa taagcaagtt
     1081 gcaggttgca agaggtatag attgcgagaa agcgggatag cgagatggag aaaactatcg
     1141 aagaagattt attaaggtcg atatcctaat gactatgatc gctaaaccga atattaaacc
     1201 ggcaatccag aacattaact taagcgggtc aactaccttg cccaaccctt gtttagccct
     1261 aactagatgg tcggtcatag actaccaaga tgctggcacc gctcaagcag ctaaacgcta
     1321 ctccccacat gaggtctgcc gcagcgttaa accacaaagg gccaccatat tgatagatag
     1381 ctcttccgat acctccagag ataaaggcta ccacgaaaag gataatcagg attctcttaa
     1441 agcgtgtgag tgtattattc aaagacattg attcctcgaa gctaatcact tttcactcat
     1501 cacctgaata accgcctgca cgatcgattc gttcggcccg acgatgatgc gcgtgtgatc
     1561 gtcgg
//
LOCUS       DASRTJ010000513         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_335656,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000513 DASRTJ010000000
VERSION     DASRTJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335656"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFF70_10365"
     CDS             <1..469
                     /locus_tag="VFF70_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HZY45140.1"
                     /translation="YLIDHGLNVRAHQLNGWWIDTGKMSDILEANRLILDVLAPQRAG
                     QVDKASHIEGRVVLEKDAVVINSTIRGPVIIGERTRIENAFVGPYTSIHHDCVLQNCE
                     IEHSVVLENTHISDTTARIADSLIGRNVEIGRSGGQTKTLKIMLGDYSKVGIL"
     gene            693..>1765
                     /locus_tag="VFF70_10370"
     CDS             693..>1765
                     /locus_tag="VFF70_10370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZY45141.1"
                     /translation="MASPLRIIPLGGLGEVGKNMTAFEYGKNIILIDCGIMFPENDML
                     GIDFIIPDWSYLRDKRDLIRGIIITHGHEDHTGGLPYLLKEFPEIPVFATKLTRGLIE
                     VKLKSHKILDDARLNTIEAGGHLDLGPFALEFFRVAHSIPDGVGVGITTPAGLIVHTG
                     DFKFDHTPVDGKPTDFAKLAEFGGRGVLALLSDSTNADQPGTTPSERVVDTAFAEVFR
                     EATGRIIVGTFASLISRIQQVGNAAVKNNRKLALAGTSMVENVKMAQRLGYLDFPDDL
                     IVRLEDTKKMAADRVVIICTGTQGEPSSVLARMSLGTHSQLQVERGDTIIMSSHPIPG
                     NEEMVHRTINRLFQRGANVLYDP"
BASE COUNT          412 a          489 c          462 g          402 t
ORIGIN      
        1 atacctgatc gatcacggct tgaacgtgcg cgcgcatcaa ctcaacggtt ggtggatcga
       61 taccggcaag atgtcggaca ttctcgaagc caaccgcttg atcctggatg tgctcgcccc
      121 acagcgcgcc ggccaagtgg acaaagcctc gcacatcgaa ggccgcgtcg tgctggaaaa
      181 agatgcagtt gtgatcaaca gcaccattcg cggcccggtg atcatcggtg aacggacgcg
      241 catcgagaat gcgttcgtcg gaccgtacac ttccattcat catgattgcg tcctccaaaa
      301 ttgcgagatc gagcatagtg tcgtgttgga gaacacgcac atcagcgata ccaccgctcg
      361 gatcgccgat agcttgatcg ggcgtaacgt cgagatcggc cgatcgggcg gccaaaccaa
      421 gacactcaag atcatgttag gcgattacag caaagtaggc attctataag acttctaaag
      481 tctaagtcat tttttgacag cgcgcgtttt atgttgtaca ctcttgtgtg tgggtgaact
      541 gaaacatccg taggaaaatt attgaccgcc ggggtgaaac agactcttgg cggtttttgt
      601 tttggcctgg cattgggatg ccatgcctgc acaatcatta atcacagcag caacgacatc
      661 cccatgtcgt tgcattagga gtaacttttt caatggcatc acctttaaga atcatcccgc
      721 tgggcggatt gggcgaagtc ggcaagaaca tgaccgcctt cgaatatggc aagaacatta
      781 tattgatcga ttgcgggatc atgttccctg aaaatgatat gctgggtatc gatttcatca
      841 tccccgattg gtcctatctg cgcgacaagc gcgatctcat tcgcggcatc atcatcaccc
      901 acggtcacga agatcacacc ggcggcctgc cgtatctgct caaagaattt ccggagattc
      961 cggtcttcgc caccaaattg acacgcggct tgatcgaagt caaactcaag tcgcataaaa
     1021 ttctggacga cgcccgctta aatacaatcg aggccggcgg ccatctggat ctcggcccgt
     1081 ttgctctaga atttttccgc gtcgcacatt cgatcccgga tggcgtcggc gtgggcatca
     1141 ccacaccggc cggtttgatt gtccatactg gcgatttcaa attcgatcac acaccggtcg
     1201 atggcaagcc aaccgatttc gccaaactcg ccgagtttgg cggacgcggt gtgctggccc
     1261 tgctctcaga ttcgaccaat gccgatcaac ctggcaccac tccctcggag cgcgtggtcg
     1321 atacggcctt cgcagaagtc tttcgtgagg ccacaggccg catcatcgtc ggcacattcg
     1381 cttcattgat ctcgcgcatt cagcaagtgg gtaatgcagc cgtgaagaat aatcgcaaac
     1441 tcgctttggc cggcaccagc atggtcgaga acgtcaagat ggcgcagcga ctgggctatc
     1501 tggatttccc cgatgatttg atcgtgcgac tggaagacac caagaagatg gcggccgatc
     1561 gtgtcgtgat catatgcact ggcacgcagg gcgaaccatc gtcggtgctc gcgcgcatgt
     1621 cgctcggcac ccactcgcag ttgcaagtcg agcgcggtga tacaatcatc atgtcttcgc
     1681 accccattcc gggcaatgaa gaaatggtgc atcgcaccat caatcgactg ttccagcgcg
     1741 gcgcgaatgt gttgtacgat cctat
//
LOCUS       DASRTJ010000514         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_70969,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000514 DASRTJ010000000
VERSION     DASRTJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70969"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFF70_10375"
     CDS             complement(<1..948)
                     /locus_tag="VFF70_10375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45142.1"
                     /translation="MNTHQRWIILPIFFLAVALRLSTITTKSLWVDEVNSLQQASWTR
                     DVILSNPYHDPHPPGYYLFLHEWIQRFGTSELWLRLPSAIFGALSVLVVYALGRSIGS
                     PRLGAWAAFLLAIDPIGIWYSQETRMYAMLAFFALCGTLVTALMLKRSTIWAWPIAVL
                     TFAAAMYLDYGGLIFWGVSIVVTIIAMIWLRAWKPARFALWLTAQVAIALLYLPQWPK
                     AFDTISSLAQGYIFQLIAEGLQRLGLKIDQQNLPIILIAVGAVMGLAFLIIFIRFMRH
                     RTGSGTSSNRDNRLIIIAILIAYVAWLLLAAAPRFYSIKR"
     gene            complement(1126..>2525)
                     /locus_tag="VFF70_10380"
     CDS             complement(1126..>2525)
                     /locus_tag="VFF70_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HZY45143.1"
                     /translation="KFDEVYPAARSGAGLLAIAPQLLGSDRPRTYLVLIQNEDELRPS
                     GGFISAAARVTLSAGKIISLTVIDGNLVDDYARKPYDFPPDPLFTVMGSELWLFRDAN
                     WSPDFPTSAREAAHLFDYGLGGGPFDGVIAINQRVVQSIVAAIDPVPIDNNTPALTAQ
                     NLERSLRDAWGPQNYDVTTQRKDFIAKMSQAIMQRLLHSPEAVRWPILARGLLDRLDS
                     HDLLITLIDPALDQSTFSDWNGSLQKSSGDYLMVVDTNLGFNKASAAISQTISYAVAI
                     ESDRTIQAGVTIDYRNANPPQSGCQHFTAKYDITTTYETLIDRCYYDYLRVLAPANAQ
                     FSDSTPHAVPTEYLVTGIGTDGSISITNELDKTSFGTFFVVERGAALHTAVSYSLPAT
                     LLTRDRDQWVYHLIWQKEPGARAWPIEFAIAWPANWKLVSASPEPKSISARSLIIARN
                     LNTDLDIEVRFNEQQ"
BASE COUNT          603 a          654 c          736 g          532 t
ORIGIN      
        1 gcgcttgatc gaatagaagc gcggcgcggc ggccagcaat aaccatgcaa cgtacgcgat
       61 caagatggcg atgatgatca atcggttgtc ccgattggac gatgtcccgc tgccagtgcg
      121 atggcgcata aaacgaataa agatgatcag gaacgctaaa cccattactg cgccgacagc
      181 gatcaatatg atcggtaaat tctgctgatc gatcttcagg ccgagccgtt gcaagccttc
      241 ggcgatcagc tggaagatgt aaccctgagc cagcgacgaa atggtgtcga acgcctttgg
      301 ccattgcggc aggtagagca gagcaatcgc aacttgcgcg gtgagccata gggcaaatcg
      361 agcaggcttc caggcccgaa gccagatcat cgcgatgatc gtcacgacga tcgacacgcc
      421 ccaaaagatc aatccgccgt aatcaagata catcgccgca gcgaatgtca acacggcgat
      481 cggccatgcc caaatcgttg atcgcttgag catcagcgcg gtaacgagcg tgccgcatag
      541 cgcaaagaaa gcaagcatag catacatgcg cgtttcttgt gaataccaaa tgccaatcgg
      601 atcaatggcg agcaaaaacg cggcccacgc gccaagccgc ggcgagccaa tcgatcgacc
      661 cagtgcgtac actaccaaca cactcaacgc accgaagatc gccgagggca atctcagcca
      721 aagttcagaa gttccgaagc gttgaatcca ttcatgcaaa aacaaatagt agccgggcgg
      781 atgcggatcg tgataggggt tggatagaat cacatcgcgc gtccaactgg cttgttgcag
      841 gctgttgact tcatccaccc acagactttt tgtggtaata gtcgacaggc gcagggctac
      901 ggctaggaag aagataggca ggatgatcca gcgttgatgt gtgttcattt caccaatccg
      961 ctgaatgtcg cttcgacgag cagcgcgagg agaagtcccg gcatcgaacg gcgctcgacg
     1021 ctgaggtgaa ctgagatttc ggccattccc aacactctag agtgttggtg gccggctagc
     1081 cgtcgccgcg aaatgatgcg gctcgaaatg acagttgcgg atctattatt gctgctcatt
     1141 gaacctcacc tcaatatcga gatcggtgtt caggttgcgg gcaatgatca gcgatcgtgc
     1201 gctgatcgat ttgggttcgg gtgaagcgga aactaatttc caattggccg gccacgcaat
     1261 cgcgaactcg attggccagg cgcgtgcgcc cggttctttc tgccagatca aatgatagac
     1321 ccactgatct cgatcgcgtg tgagaagtgt agcgggcagt gaataactga cggcggtgtg
     1381 taatgctgcg ccgcgctcta ctacaaagaa tgtgccgaaa gaagttttat ctagttcgtt
     1441 ggtgatcgaa atcgatccgt cagtgccgat gccggtgacg agatattcgg tcggcacagc
     1501 gtgcggtgtc gagtcactaa attgtgcgtt ggcgggcgcg agcacacgta gataatcata
     1561 gtagcagcga tcgatcagag tttcatacgt tgtggtgatg tcatacttcg cggtgaaatg
     1621 ctggcagccg gattgcggcg gatttgcgtt gcgataatcg atcgtgacgc ccgcctgaat
     1681 ggttcgatcg gattcgatcg cgactgcgta gctgatcgtt tgggagatgg ccgcactcgc
     1741 tttgttgaag ccgaggttgg tatcgacgac cattagataa tcgccggaag atttttgaag
     1801 cgagccattc caatcggaaa aagttgattg atcgagcgcc ggatcgatga gcgtaatcag
     1861 cagatcgtgg ctgtccaatc gatcaagcaa gccgcgcgcc aagatcggcc agcgtacggc
     1921 ttcgggtgag tgaagcaggc gctgcatgat cgcttgcgac attttggcga tgaagtcttt
     1981 gcgttgtgtg gttacgtcgt agttctgcgg accccacgca tcgcgcaaag acctttccag
     2041 attctgcgcc gtcaaggccg gggtgttgtt gtcgatcggt accggatcaa tcgcggcgac
     2101 gatcgactgc acgacgcgtt gattgattgc gatgacgcca tcgaatgggc caccgcctag
     2161 gccgtaatca aacaggtgcg cggcctcgcg cgccgatgtg ggaaagtccg gcgaccagtt
     2221 cgcgtcgcgg aacagccaca actctgaacc catcacggta aagagtggat caggcgggaa
     2281 atcatacggt ttgcgcgcgt aatcgtcgac gagattccca tcgatcacag tcaggctgat
     2341 aattttgcct gcgctgagcg taacgcgtgc cgccgcgctg atgaagccgc cgcttggccg
     2401 cagttcgtct tcgttttgaa tcagcaccaa ataggtgcgc ggtcggtcgc tgcccaataa
     2461 ctgcggcgca atcgctaaca ggccggcacc cgatcgtgcg gccggataaa cttcatcgaa
     2521 tttat
//
LOCUS       DASRTJ010000515         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_354724,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000515 DASRTJ010000000
VERSION     DASRTJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354724"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..521
                     /locus_tag="VFF70_10385"
     CDS             300..521
                     /locus_tag="VFF70_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HZY45144.1"
                     /translation="MYYDDHQPPHFHAIYGGAEAQIAIDPIYILRGDLPDRAVSMIIE
                     WAALHQRELSDNWQRMRSNQQPQKVKPLQ"
     gene            536..808
                     /locus_tag="VFF70_10390"
     CDS             536..808
                     /locus_tag="VFF70_10390"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZY45145.1"
                     /translation="MFPRIIQVRYIQDYTLELSFSDGVHGKIDFRPKIVGRGGVFKSL
                     EDVELFEQVKVDSEVGTIVWPNEVDFDPDVLYSEITGIPIQVPQTV"
     gene            complement(943..1644)
                     /locus_tag="VFF70_10395"
     CDS             complement(943..1644)
                     /locus_tag="VFF70_10395"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZY45146.1"
                     /translation="MKVHLKIQRFNSETDVKPYWSEHDVEAEPNDRVLDVLNQVKWYS
                     DGALTYRRSCAHGICGSDAMRINGRNMLACKALIKDVGKKLTIQPLLGMPIIKDLVVD
                     MEGFFAKYRSIKPYLINDEPEPERERLQTIEQRERFDDTTKCILCAACTTSCPSFWAN
                     DEYVGPASIVQAHRFVFDSRDHGAEERLEVLSDMMGVWRCRTAYNCVEACPRGINITQ
                     AIGEVKNAIVNGLDK"
     gene            complement(1662..3437)
                     /gene="sdhA"
                     /locus_tag="VFF70_10400"
     CDS             complement(1662..3437)
                     /gene="sdhA"
                     /locus_tag="VFF70_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZY45147.1"
                     /translation="MTVQHEFDAIVVGAGGAGMMAALHCSKTVKSTAVISKLYPTRSH
                     TGTAQGGIAAPLGNMEEDHWEWYMYDTVKGGDYLVDQDAAEVLAKESIDSVIELEHMG
                     LPFDRTPEGKIEQRRFGGHSSNYGEKPVMRSCKAADRTGHMILQTLYQQCIKQNVQFF
                     DEYQVIDVIVEDNICRGVVAVQILTGETHIFHAKATLLAVGGFGRVWSVTSNAHSLTG
                     DTVASVYRHGLPLEDMEFFQFHPTGVYKMGILLSEALRGEGAIFINDKGERFMERYAP
                     TMKDLASRDVCSRSIYLEIRDGRGIGGKDYVYIDVRPETINKYKSGPGGKPVTAEHLE
                     AALPDVFEMARVYLGVEPMKEGVPVQPTAHYAMGGVPTNLNCEVLADETGKVVKGLYA
                     AGECACVSVHGANRLGTNSLVDLVVFGRRGGKAMAEFVKHADFAPLPKNPEAYTLAEF
                     NRIRKGTGPERPAIIRAEMQKIMMADVGVFREGPTIQKALDKIKELQTRYQNISIDDK
                     GSKFNTDLFEAWELGCLLDLAEVISVSAMYRTESRGGHSREDFPKRDDVNWLKHTFVT
                     RKNGQTSVAYKPVTITKFQPKERTY"
     gene            complement(3439..4371)
                     /gene="sdhC"
                     /locus_tag="VFF70_10405"
     CDS             complement(3439..4371)
                     /gene="sdhC"
                     /locus_tag="VFF70_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZY45148.1"
                     /translation="MSVSNPAKNQSALPKDYLEDGTLYKGGNGMLTWLAHRMSGVAIV
                     YFLTLHIVEAIQLFGGPANYSEATAVYKQPWFRPFEWLLVMAVIYHALNGLRVMLYDT
                     WPRTTRYHRQIFWIGVGLFVLATPMVGYAMLKSVFGMSLQEIFASMNGLGYALAIVLP
                     VALPVLYVAWRGSGLSNGPLIVSQSSSRPAPMKNNFERLSWQFMRISGVILIVLVFWH
                     LIIMHFINDISNITGDFVFARFAENPTWIAVDLSMLVLAWLHGLNGVRIVITDYMRRG
                     YIRRVVLMALFAFGLVWLVAGAIVLFRLPNGLGA"
     gene            complement(4526..5797)
                     /gene="icd"
                     /locus_tag="VFF70_10410"
     CDS             complement(4526..5797)
                     /gene="icd"
                     /locus_tag="VFF70_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZY45149.1"
                     /translation="MDGQKITIKNGKLIVPDNPVLPFIEGDGTGPDIWRASVRVLDAA
                     VKKAYGGQRSIIWKEVLAGQKAFDQTQNWLPAETVEAFREYLVGIKGPLTTPIGGGIR
                     SLNVALRQMLDLYVCLRPVRWFKGVPSPVKHPEKVDVVIFRENTEDIYAGIEFESGSE
                     AAKKFLEMFKTNFPKEFSKIRFPDSSGIGIKPVSREGTERLVRAAINYALANNRKSVT
                     FVHKGNIMKFTEGAFRNWGYALAEREYADQVYTWEQWERTKAVKDEAAANAEQKSALA
                     SGKILIKDAIADIALQQFLTRPDEFDVIATMNLNGDYESDALAAQIGGIGIAPGGNIN
                     YLTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYMGWIEAADLIIKGMDGAIG
                     AGSVTYDFARQMDGAREVKCSEFGDAVIAKM"
BASE COUNT         1329 a         1677 c         1524 g         1402 t
ORIGIN      
        1 ggttttgcgc gcacgctcgg tatgccgccg cattttcagt atcaagattc caagggcaat
       61 tttatttggg agtggcgcat ggctgatcgg gatgcgcgct ggcaagcgat gcagggcgtg
      121 ccggccttca agaatttgaa acggttatat gaagtgatcg agtacgtgta atccgattgc
      181 tcgtcatgat gaaaatcctg ctcacttttt agcgggattt ttattttcta tcgcccgaaa
      241 gaaatgatat aatcagcaca atgccagaga ttagccgctt tttcggaatc attgtgtgaa
      301 tgtactatga cgatcaccag ccgccgcatt ttcacgccat ttatggtggt gcagaagcgc
      361 agattgctat cgatccgatt tatatcttgc gcggcgactt gccggacaga gccgtatcga
      421 tgatcatcga gtgggccgca ttgcatcaac gcgaattgtc agataattgg caacgcatgc
      481 ggagtaatca acagccacaa aaagtgaagc cgttgcaata agaggaggca tggccatgtt
      541 tccgcgtatc attcaagttc gatacattca agactacaca ctcgagctat cgttttccga
      601 cggcgttcac ggcaagatcg atttcaggcc aaagattgtc ggacgcggcg gtgtgttcaa
      661 atcgctcgaa gacgttgaac tcttcgaaca agtgaaagtt gattcggaag tgggcaccat
      721 cgtctggcct aacgaagtcg attttgatcc agacgttctg tatagcgaga tcactggtat
      781 tccaattcaa gttcctcaaa cagtctaaga atctgaaaca ggaacgcccc gctgatgcta
      841 aatcagcggg gcgttccttg ttttgatgca atggcgatgg catggggatg ccatcgctac
      901 gatttagatt cgcagacgcg gcatccctct gccgcgttga tgttatttat ccaacccatt
      961 cacaatcgca tttttgactt cgccgatcgc ctgggtgatg ttgatgccgc gcgggcaggc
     1021 ctcgacacag ttgtacgccg tgcggcagcg ccacacgccc atcatgtcgc tcaacacttc
     1081 caggcgctct tctgcgccgt gatcgcggct gtcgaatacg aaacggtgcg cttgcacgat
     1141 cgaggccgga ccgacatatt catcgttcgc ccagaatgac gggcacgatg tggtacaggc
     1201 cgcacacaga atgcatttag tcgtatcgtc gaaacgctca cgctgttcaa tcgtttgtag
     1261 acgttcgcgc tccggctccg gctcgtcatt gatcaagtat ggcttgatcg atcgatactt
     1321 ggcgaagaag ccttccatat cgacgacgag atctttgatg atcggcatgc ccagcagcgg
     1381 ttggatggtg agtttcttgc cgacgtcttt gatcaacgct ttgcaggcca gcatgttgcg
     1441 gccattgatg cgcatcgcgt ccgatccgca gatgccgtgc gcgcacgaac gacgataggt
     1501 cagcgcgcca tcgctatacc atttcacctg attaagcaca tccagcaccc gatcgtttgg
     1561 ttcggcctcg acgtcatgct cgctccaata cggcttcaca tcggtttcgg aattgaagcg
     1621 ttgaattttc aaatgaactt tcatgttgtc tcattctccc gttaatacgt tcgttctttc
     1681 ggctggaact tcgtaatggt tacgggctta tacgccaccg aggtttgacc attcttgcgc
     1741 gtgacgaagg tatgtttaag ccaattgaca tcatcgcgct tggggaaatc ttcgcgcgaa
     1801 tgtccgccgc ggctctcagt gcgatacatc gccgagaccg atatgacttc agccagatcg
     1861 agcaagcagc ccaattccca ggcttcaaac agatcggtgt tgaatttgct gcccttatcg
     1921 tcgatcgaaa tattctgata gcgcgtctgc agttccttga tcttatcgag cgctttctga
     1981 atggttggtc cttcgcggaa cacaccgaca tcggccatca taatcttttg catttcagcg
     2041 cggatgatcg ccggccgttc cgggccagta cctttgcgaa tccgattgaa ctcggcgagc
     2101 gtgtacgctt cgggattctt gggcagcggg gcgaaatcgg cgtgcttcac aaattcggcc
     2161 atcgctttgc cgccgcgtcg accgaacacc accagatcga cgagcgagtt agtgcccaat
     2221 cgatttgcgc catgcacgga aacgcaggcg cattcacccg cagcgtacaa gcctttcacg
     2281 actttgcctg tctcatcggc caacacttcg caattcagat tggtcggcac gccgcccatc
     2341 gcataatgcg cggtcggttg aacgggcacg ccttctttca tcggctccac acccagatac
     2401 acgcgcgcca tttcaaacac atccggcagc gcggcttcaa gatgttcggc ggtgactggc
     2461 ttaccgcccg gcccgctttt atatttgttg atcgtttccg gacgaacatc gatgtacaca
     2521 tagtctttgc cgccgatgcc gcgcccgtcg cggatctcca gataaatcga tcggctgcat
     2581 acatcccgcg aggccaggtc tttcatcgtc ggcgcatagc gctccatgaa gcgctcgcct
     2641 ttatcgttga taaagatcgc gccttcgccg cgcagcgctt ccgacaacaa aatgcccatt
     2701 ttgtagacac cggtcggatg gaattgaaaa aactccatgt cttccaacgg caagccatgt
     2761 cgataaaccg acgcgacggt atcgccggtc agtgaatgcg cattcgaagt taccgaccat
     2821 acgcggccaa agccgccgac cgcgagcagc gtggctttcg catggaagat gtgcgtctcg
     2881 ccggtcagga tttgcaccgc gacgacgccg cgacaaatat tgtcttcgac gatcacatcg
     2941 atcacttgat actcgtcgaa gaactggacg ttctgcttga tgcactgctg atacagcgtc
     3001 tgcaagatca tgtggcccgt ccgatcggcc gccttgcacg atcgcatcac gggcttctcg
     3061 ccgtaattgc tgctgtgccc gccgaaccgc cgctgctcga tcttgccctc gggcgtgcga
     3121 tcgaacggca ggcccatgtg ctccagttcg atcaccgaat cgatcgattc tttcgccagc
     3181 acttcggccg catcttgatc gaccagataa tcgccgccct tgacggtgtc atacatgtac
     3241 cattcccaat gatcttcttc catgttgccc aacggcgcgg cgatgccgcc ctgcgctgtg
     3301 ccggtatgcg atcgagtggg atacagctta ctgatgacgg ctgtgctctt gacggtcttg
     3361 gaacaatgca gtgcggccat cataccggcg ccccctgcgc cgacgacgat cgcatcgaat
     3421 tcgtgttgaa ctgtcatgtt aagctcccaa cccattcggt aatctgaata aaacgattgc
     3481 gcccgccacc agccagacca agccgaaggc aaacaaggcc atcaacacaa cccggcgaat
     3541 atagccgcgc cgcatgtaat ctgtgatgac gatacgcaca ccgttgaggc cgtgcagcca
     3601 ggccaagacc aacatcgata gatcgacagc gatccaggta gggttttctg cgaagcgcgc
     3661 gaacacaaaa tcgccggtga tgttgctgat gtcgttgatg aagtgcatga tgatcaaatg
     3721 ccagaagacc agcacgatca agatcacacc ggagatgcgc atgaactgcc aggacaagcg
     3781 ctcaaagttg tttttcattg gcgcgggtcg gctgctggat tgcgaaacaa tcagcgggcc
     3841 atttgacaaa cccgatccgc gccacgcgac gtataaaacg ggtagcgcca ccggcaggac
     3901 aatcgctaaa gcgtaaccga ggccgttcat cgaagcaaaa atttcttgaa ggctcatccc
     3961 gaagactgac ttgagcatcg cataaccgac catcggtgtg gcgagcacga atagtcccac
     4021 accgatccaa aagatctggc gatgatagcg cgtggtcctg ggccatgtgt catataacat
     4081 gacgcgcagg ccgttcaagg cgtgatagat cacggccatg accaatagcc attcgaacgg
     4141 acggaaccag ggctgcttat ataccgcggt cgcttcgcta tagttcgccg ggccgccgaa
     4201 gagttgaatc gcttcgacga tgtgcagcgt caggaaataa acgattgcca caccgctcat
     4261 gcgatgcgcc agccaggtca acatgccatt gccgccctta taaagcgttc cgtcttcaag
     4321 ataatctttg ggcagggcgg actggttttt tgctggatta ctgacagaca tgctgtcccc
     4381 tttccttaaa gagtgaggtt caatcaaagc cgagcttgcg cggttcaata ttgaaaagtt
     4441 acgccttgtt tattatagat cacctgattg gcttcggcaa agtgagccaa acaaaaaggc
     4501 gagacctaga gtctcgcctt atgcttcaca tctttgcgat cactgcatcg ccaaactcac
     4561 tacacttcac ttcgcgcgcg ccgtccatct gccgcgcaaa atcatatgtg actgacccag
     4621 cgccgattgc gccatccata cctttgatga tcagatcggc ggcttcgatc cagcccatat
     4681 agcgcagcat catttcgccc gatagaatga ccgaacccgg attgacttta tcgaggttcg
     4741 catatttcgg tgccgtaccg tgcgtcgctt caaagactgc atgacccgtt aagtagttga
     4801 tgttgccgcc cggcgcaatg ccgatgccgc cgatttgcgc ggcgagcgcg tcggattcgt
     4861 aatcgccgtt gaggttcatc gtcgcgatga cgtcgaactc atcggggcgg gtcaggaatt
     4921 gctgaagcgc aatatccgca atcgcatcct tgatcaaaat cttgccggac gcgagcgcag
     4981 atttttgttc ggcattcgcc gccgcttcgt ctttcacggc tttcgtgcgt tcccattgct
     5041 cccaagtgta gacctgatcg gcatattccc gctcggccag cgcatagccc caattgcgaa
     5101 acgcgccttc agtgaatttc atgatgttgc ccttgtgtac gaaggtaacg ctcttgcgat
     5161 tatttgccag cgcataattg atcgcagccc ggacgagccg ctcggtgccc tcgcgcgaaa
     5221 ccggcttgat cccaataccc gatgaatcag ggaagcgaat cttactaaat tccttgggaa
     5281 aattcgtctt gaacatctcg agaaatttct tggccgcttc gctgccggat tcaaattcaa
     5341 tgcccgcgta aatgtcttcg gtgttctcgc ggaagatcac cacgtcgact ttttccggat
     5401 gcttcaccgg tgagggtacg cctttgaacc agcgcaccgg ccgcaagcac acatacagat
     5461 cgagcatctg ccggagcgcc acgttgagcg atcggatgcc acccccgatc ggggtggtca
     5521 acggcccttt gatgccgacc aagtattcac ggaatgcctc aaccgtttca gcgggcagcc
     5581 agttctgcgt ttgatcgaac gccttctgtc ccgctaacac ttctttccag atgatcgatc
     5641 gttggccgcc ataagctttc ttcaccgcgg catcgagcac gcgcacactc gcgcgccaaa
     5701 tatccggccc cgtcccatcg ccttcgataa acggcagcac cggattgtcg ggcacgatca
     5761 atttgccgtt cttgatcgtg atcttttgtc catccatgat gaattgctcc tatcgtaaac
     5821 tatcctcccg caggtcgctt gatgaagagg aattcagcat tgagacctac gggaggatga
     5881 atgaagtcga tcgggattat agcatgaatc atcgggcagg cgcggcgcga tc
//
LOCUS       DASRTJ010000516         5499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_71137,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000516 DASRTJ010000000
VERSION     DASRTJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71137"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1416
                     /gene="ssnA"
                     /locus_tag="VFF70_10415"
     CDS             73..1416
                     /gene="ssnA"
                     /locus_tag="VFF70_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HZY45150.1"
                     /translation="MLITHARIATFGKSLQVIDDGAIYIEDDLIKEVGSNRELIAQHP
                     NVETIDAKGQLALPGLICAHTHFYGAFARGLSIPGKPARNFVEILQKLWWRLDRALDE
                     ESIRLSGLVCLVDAIRNGTTTLIDHHASPNIIDGSLDILAETVNQSGLRVCECYEVTD
                     RNGLDGALAGIKENVRFAKSMRSPLLAASFGLHASFTVGPSTLERSISEAKSIGLGFH
                     IHVAEDSADEIDSERKYGERVGDRLNAAGMLGEKTLSAHCVHVNDREIALLAKTKTKV
                     SHQPRSNMNNAVGTAPVQHMLDQGVCVGLGNDGFSNNMFSEMKAAYLVHKSVANDPRA
                     MSGETVTNMAYQNNAQIASLFWTKPVGEISIGAYADIILLDYYPHTPLTSGNLPWQIA
                     FGIDGSHVATTIVGGKVLMRDRELLTLDEEHIAAQARAIAPKVWQRFEEITQHEA"
     gene            1557..2222
                     /gene="npdG"
                     /locus_tag="VFF70_10420"
     CDS             1557..2222
                     /gene="npdG"
                     /locus_tag="VFF70_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZY45151.1"
                     /translation="MTLPTIAILGGTGKEGGGLALRWAHAGYSIIIGSRDADRARLAA
                     QEMNTQLGGGSILRGMNNIEATQVSDIAVLSVPYEAHQTTLESIKDAVQGKILIDVTA
                     PLDPDNKRRVRKIAAGSASEEAQKFLGDGVKVVSAFQNISYTHLSNLAEALHCDVLVC
                     GNDKEAKRLTIELAQAAKMQAYDAGSIENSVVAEGLTAVLININIQFKIKNAGIQITG
                     VLR"
     gene            2325..2912
                     /locus_tag="VFF70_10425"
     CDS             2325..2912
                     /locus_tag="VFF70_10425"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HZY45152.1"
                     /translation="MCNFNVRFRRIAALCGLISPIISLSTILISVVMIADFSWQANDL
                     SDMGVSATPNLFNFGLIVTGLLSVIFAVGVTRWIGPDRSAHIGSAALIIGSFDVALIG
                     VITEAYGPLHFWVSVAYFVLVPISYLFLSRNLWRKGKKIASRLSIAAGLTVLMMMALR
                     KVLTHDSGVAVPETLSAMITNAWTFGLALMLWLDS"
     gene            2909..3673
                     /gene="cofE"
                     /locus_tag="VFF70_10430"
     CDS             2909..3673
                     /gene="cofE"
                     /locus_tag="VFF70_10430"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZY45153.1"
                     /translation="MTDSISIIPLAGIPLIQPGDDLAQILGDAIDRLESPVKSNDVLV
                     IAQKIVSKAENRFVDLDRVTPSPRAIEIAKQCDKDPRVIEVILWDTHEVVRLRPGLII
                     VEHKLGFVSANAGVDRSNVAPDGIDRVLRLPENPDRSAAHIRSVLRDRFQIDLSVIIA
                     DSHGRPHRLGTIGVAIGLAGLPGVEDWRGRTDLFGYTLQHTEVGLADQIASAATLLLG
                     QAAEKIPAVIVRGAIYEKRDGSAQEINRPREMDLFR"
     gene            complement(3730..4692)
                     /locus_tag="VFF70_10435"
     CDS             complement(3730..4692)
                     /locus_tag="VFF70_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HZY45154.1"
                     /translation="MNTPPRKLLDQVRDAIRVKHYSDRTEKTYVDWIKRYILFHNKRH
                     PVEMGEVEVQAFIRHLAVDRKVSASTQNQALSAVIFLYRHVLKKGLALPLKTVSAERR
                     KRLPSVLTKEEAMLVIVKMQGTPQLMAKLLYGTGPRLMECLRLRIKDVDFANHIIIVR
                     DGKGEKDRSTIMPDTITGDLKAHILKTKTIHDKDLREGYGEAPLPYALSRKYPNASRE
                     WAWQFIFPASQRSTDPISGKIMRYHIHESVLQRAVKDAAQTTSLAKHISPHIFRHSFA
                     THLLQNGYDIRTVQELLGHKDVKTTMIYTHVLQRGGLAVRSPLD"
     gene            4849..5487
                     /locus_tag="VFF70_10440"
     CDS             4849..5487
                     /locus_tag="VFF70_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZY45155.1"
                     /translation="MTNVFVNIGLSLDGYMAPEGMTIENWEKPEYKNWGAKWGELMAW
                     IINQQYIRENLKLGPGGETGPVNDMVRNTTERIGANIMGKRMFEQGEVSWPEEAPFHT
                     PVYVLTHEKREPWVRPGGTTFYFVNDGPESALEQARESAGGRDIRISGGADVIQKYLN
                     MGVIDELEIALAPVLFGGGRRLFENLHEAVPQFRIDKVFDSPTATHLRFVRE"
BASE COUNT         1309 a         1422 c         1467 g         1301 t
ORIGIN      
        1 tgtctctttg ctttcatgtt acaatcctcg ccgatccgaa attcaacttc cattatctaa
       61 tttccaacca ccatgctcat tacacacgct cgaatcgcta cgttcggaaa aagcctgcaa
      121 gtcattgacg atggcgcgat ctatattgaa gacgatctta tcaaagaggt cggatcgaat
      181 cgtgaattga tcgcccagca tccgaacgtt gagacgatcg acgccaaggg ccaactcgcc
      241 ctgcccggct tgatctgcgc gcacactcat ttctatggcg cgtttgcgcg tggcctgtcg
      301 atcccgggta aaccggccag gaacttcgtc gagatattgc agaaactttg gtggcggctc
      361 gatcgtgcgc tggatgaaga gagcatccgc ttgtccgggc tggtctgcct agttgatgcg
      421 atccgcaacg gcacgacaac gttgatcgat caccatgcca gcccgaatat aatcgatggt
      481 tcattggata ttcttgccga gaccgtaaat caatcgggct tgagagtgtg cgaatgttat
      541 gaagtcactg atcgaaacgg actggatggc gcgctggctg gcatcaaaga gaatgtccgc
      601 ttcgctaaat cgatgcgctc gcccttgctt gccgcatcct ttggactgca tgcctcattt
      661 acagttggcc cgtcaacttt ggaacgctcg atcagcgaag ccaaatcgat cgggctgggc
      721 tttcatattc acgtcgctga agacagcgcg gacgagatcg acagtgaaag aaagtacggc
      781 gaacgcgtcg gcgatcgatt aaacgcggcc ggtatgttgg gcgagaaaac tttatcggca
      841 cactgcgtgc acgtcaacga tcgcgagatt gctttactcg ctaaaacaaa aaccaaagtc
      901 agtcatcaac cacggagcaa catgaacaat gcggtcggca ctgcaccggt acagcacatg
      961 cttgatcaag gtgtgtgtgt cggtttaggc aatgatgggt tttccaacaa catgttcagt
     1021 gaaatgaaag cggcctatct cgtgcacaaa tccgttgcga acgatccgcg cgctatgagc
     1081 ggggagacag tgacaaacat ggcttatcag aataacgccc agatcgcctc actgttctgg
     1141 accaagcccg tcggcgaaat ttcaatcggc gcatatgctg atattatttt gctcgactat
     1201 tatccacata cacccttgac ttcaggtaac ctgccgtggc agatcgcgtt cggtatcgat
     1261 ggttcacacg tggcaacgac gatcgtcggc ggcaaagtat tgatgcgcga ccgagaatta
     1321 ctcacgctcg acgaagaaca tatcgccgcg caagctcgcg cgatcgcgcc aaaagtatgg
     1381 cagcgctttg aagagataac acagcatgaa gcataaccct atcccgctga ttaatcgatc
     1441 gagaatcatc cgaggcgaca cctggcgtcg gggccgctgc accacttttt cgtgatagat
     1501 gttgcagggc gtaattacgc attaccattc actcatcact tttttacgga gaccacatga
     1561 ctcttccgac gatcgcaatc ctcggcggca ccggcaaaga aggcggcgga cttgcgctgc
     1621 gttgggcgca cgccggttac tcgatcatca tcggctcacg tgacgccgac cgcgccaggc
     1681 tagccgccca agaaatgaat actcaactcg gcggcggatc gatcctgcgc gggatgaaca
     1741 acattgaagc aactcaagtc agtgacattg cagttctctc ggtcccatac gaagcccatc
     1801 aaacgacgct ggaatcgatc aaagatgcag tccagggaaa aatcttaatc gacgtgaccg
     1861 cgccgctcga tccggacaac aaacgccgcg tgcggaagat cgcggcgggc tcggccagcg
     1921 aagaagcgca aaagttttta ggtgatggcg taaaagtggt gtccgctttt caaaacatca
     1981 gctacacgca tttgtctaat ctcgcagaag ccctgcactg cgatgtgctg gtttgcggca
     2041 acgacaaaga agctaaacgg ctgacgatcg agttagcaca agcggccaag atgcaagcct
     2101 acgacgccgg gtcgatcgag aattcggttg tggccgaggg cttgacggcg gtgttgatca
     2161 acatcaacat tcaattcaag atcaagaacg ccggaattca aatcacgggc gtgctgcgct
     2221 aatcgatccg tcattgcgat aaatgcgcaa ggccgacgct gcaagcgcat tgataaacgt
     2281 agcgattgct tcgccgcgct tcttacgacg cttgaaaaat caccatgtgt aacttcaatg
     2341 tccggttccg ccgcatcgcc gcactatgcg gactcatctc gccgatcatt tccctgagca
     2401 cgatcctgat ctcggtcgtg atgatcgccg atttcagttg gcaggccaac gatctcagcg
     2461 atatgggcgt cagcgccacc ccgaatctct tcaacttcgg cttgatcgtc accggtttgc
     2521 tcagcgtgat ttttgcggtg ggcgtcacgc gctggatcgg accagatcga tcggcgcaca
     2581 ttggcagcgc agcgttgatc atcggatcat tcgatgtcgc cctgatcggc gtcatcaccg
     2641 aagcctatgg ccccttgcac ttttgggttt cggttgctta tttcgtgctc gtacctatca
     2701 gctacctctt tttaagccgc aatctctggc gtaaaggcaa aaagatcgca agcagattat
     2761 cgatcgcggc cgggctcacc gtcttgatga tgatggctct gcgcaaagtc ttgactcacg
     2821 atagtggcgt cgccgtcccg gaaactctgt cggcgatgat cacaaacgct tggacattcg
     2881 gcctggcgtt gatgttgtgg ctcgactcat gaccgactcg atcagcatca ttcctctcgc
     2941 tggcattccg ctcatccaac ccggcgatga cttggcgcaa attttgggcg atgccatcga
     3001 tcgtttggaa tcacccgtca agtctaatga tgtcctcgtc atcgcgcaga agatcgtcag
     3061 caaggcggaa aatcgcttcg tcgatctcga tcgggtcaca ccttcaccgc gtgcgattga
     3121 gatcgccaag caatgcgata aagatccacg cgtgattgag gtcatcttat gggacaccca
     3181 tgaggttgtt cgcttgcgtc ccggcttaat catcgtcgag cataaactgg gcttcgtcag
     3241 cgcgaatgcg ggtgtcgatc gcagcaacgt cgcgcccgat ggaatcgatc gggttttgcg
     3301 attgcctgaa aatcccgatc gatccgctgc gcacatccga tcggtcttgc gcgatcggtt
     3361 ccagatcgat cttagcgtga tcatcgctga cagccacggg cggccgcatc gtctgggcac
     3421 gatcggtgtc gcgatcgggc tggcaggttt gccgggcgtg gaagattggc gcggacgcac
     3481 agatttgttt ggttatactt tgcagcacac cgaggtcggc ctcgccgatc agatcgcctc
     3541 tgcggccaca ttgttgttgg gccaagccgc cgaaaaaatt cccgcggtca tcgtgcgcgg
     3601 agcgatctat gagaaacgtg atggatcggc gcaagagatt aatcgaccgc gagaaatgga
     3661 tttgtttaga taaactagta gtcgaagtga gatgagacaa gcccgatgac gagtccatcg
     3721 gtgccgatct cagtcaagcg ggctgcgaac cgcgaggccg ccgcgttgga ggacgtgcgt
     3781 atagatcatc gtcgtcttca catccttatg gccgagcaat tcttgcacgg ttcggatgtc
     3841 gtaaccattt tgtaaaagat gtgtagcaaa ggagtggcga aaaatatgtg ggctgatatg
     3901 cttggcgagt gatgttgttt gagctgcgtc tttgactgcg cgctgtaata cgctttcatg
     3961 gatgtggtat cgcatgatct tgccagatat tggatctgtg gaacgttgcg aggcgggaaa
     4021 aataaattgc catgcccatt ccctactcgc atttgggtat ttgcgactga gagcgtatgg
     4081 caaaggggct tcaccatagc cttcacgcaa gtccttatca tgaatggttt tagtttttag
     4141 gatatgggct tttaaatcgc cagtaattgt gtctggcatg atagtagagc gatctttttc
     4201 acccttccca tctcggacaa taataatgtg gtttgcaaag tcaacatctt tgattctcag
     4261 tcttaaacat tccatcaggc gcggaccagt gccatacaag agtttagcca tcaactgagg
     4321 cgttccctgc attttgacaa ttacgagcat ggcctcttct tttgtcaata ccgatggaag
     4381 cctttttcgc cgctcagcac taacagtctt taaaggcaag gccaaacctt ttttcaaaac
     4441 atgccgatag aggaatataa ctgcgctaag cgcttgattt tgagtagagg cggatacctt
     4501 tcgatctaca gcaagatgcc tgataaatgc ctgcacttcc acctccccca tctcaacagg
     4561 atggcgtttg ttatgaaaca aaatgtaacg cttgatccag tccacatagg ttttttcggt
     4621 gcggtcggag taatgtttaa ctcgtatagc atcacgaacc tgatcgagca gttttctagg
     4681 tggagtattc atcgaaatct cttgacagtt atgctgtact tgcagtatcc agatttcacc
     4741 ccactttgca gtctaaagta attagacttc atgtaatcca taggctatta gaatggatgc
     4801 aatagaattt caagttaggc cgacgtacag aatcaggaga gatgcatcat gaccaatgtt
     4861 ttcgtgaaca tcggacttag ccttgacggc tacatggcgc cggaagggat gaccattgag
     4921 aattgggaga agcctgagta caagaactgg ggtgccaagt ggggtgagct aatggcctgg
     4981 atcattaatc agcagtacat ccgcgagaac ctcaagctcg gaccaggggg agagaccggt
     5041 ccggtcaatg acatggttcg taacaccacg gagcgcatcg gcgccaacat catgggcaag
     5101 cgaatgttcg agcaaggcga ggtctcatgg cccgaggagg ctccgtttca cactccagta
     5161 tacgttctta cccacgagaa gcgcgaacct tgggtccggc cgggcgggac gacattctac
     5221 ttcgtcaacg acggcccgga gagcgccctt gagcaggcta gagagtctgc gggcggtcgt
     5281 gatattcgca tctcgggtgg agcggacgtg atccagaagt acttgaacat gggagtcatc
     5341 gacgagctag aaatcgccct ggcacccgtg ctgttcggtg gtgggagacg tctgttcgag
     5401 aacctgcacg aggccgtgcc gcagtttcga attgataagg tcttcgatag tccaactgcc
     5461 acacacttgc gctttgtgcg tgagtgaggt ggcctacat
//
LOCUS       DASRTJ010000517         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_33011,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000517 DASRTJ010000000
VERSION     DASRTJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33011"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFF70_10445"
     CDS             <1..872
                     /locus_tag="VFF70_10445"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HZY45156.1"
                     /translation="LALPALNEEATVGKVIRTIRRALMKNHSLIDEIILIDSGSDDRT
                     REIAQRLGVPVYIHQEILPQYGAHTGKGEALWKSLHVTHGDLIAWIDTDIVNIHPRFV
                     YGVIGPLLHSDRIQFVKGFYRRPLKVGDKMQAGGGGRVTELVARPLINLFFPELSGLI
                     QPLSGEYAGWRSALEQVPFFTGYGVETGLLIDLHEKFGLRGIAQVDLEERVHHNQPLD
                     ALGRMSFAIIQVIVARLEDRHKINLLAEINRNMKIVRHEPGRYYLDLEEIGDLQRPPM
                     IQIDEYRRARSLS"
     gene            951..2462
                     /locus_tag="VFF70_10450"
     CDS             951..2462
                     /locus_tag="VFF70_10450"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZY45157.1"
                     /translation="MRKFILILALVVTACAPIAVTQPIVPTPTRSPISGPTPTPDPVW
                     ADLSPYRAAMRPEFAQDVDQFANATQYKIDLTIAPDLTSYTGSQQVHYINTETSALGQ
                     IYFRLFPNTQAYGGQLTITSLKMNGASIEPIMELGNSAMRIDLSSPLNGGAAVDFEMS
                     YQTIVPTTSITYGYDQFGLLDHILALPNFYPQIPAYDEEGWNVEIAPGDGDAVYSDSA
                     LYQVNISAPSNQIVAASGVCDRSSNATTQTLKCVSGPMRDFMIGMSADFQIASDQIDG
                     IQVNSYYLARDAEAGQNGLTTVVNSIKSYEQRIGAYPFTELDLLETPTQAGGIEYPGL
                     IVVAEDLYRQARGFQEGATAHEAAHQWWYSLVGNDQLDDPWLDESLTQFTTALYFYDQ
                     YGQSGLDGDVNQDLVRRHDRVKGTDEDKRADLPVAAYTGRQYSAIVYGKAPLFFYALW
                     QKMGDEKYNAFMQNYFQANRYGVAKVDDLLKAIETQLDKAAVDALMKQWITTP"
     gene            2578..>2817
                     /locus_tag="VFF70_10455"
     CDS             2578..>2817
                     /locus_tag="VFF70_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY45158.1"
                     /translation="MKQRSSAPDLFDHAAQETIARSQPLAARMRPRSLDEYVGQAEII
                     GPNRLLRRAIETDKMFSSILLWGPPGTGKTTLAMVI"
BASE COUNT          643 a          774 c          764 g          636 t
ORIGIN      
        1 gtttggcgct gcccgcattg aatgaagaag ccacggtggg caaagtgatc cgcacgattc
       61 ggcgggctct aatgaaaaat cattcactga ttgatgaaat catcttgatc gattccggtt
      121 cagacgatcg gacgcgagag atcgcacagc gcttgggtgt acccgtctat atccatcaag
      181 aaattttacc gcaatacggc gcgcacaccg gcaaaggtga agcgttgtgg aaaagcttgc
      241 acgtgaccca cggcgatctc attgcctgga tcgatacgga catcgtcaac attcatccgc
      301 gttttgtata tggcgtcatc ggaccgctgc tgcactccga tcgcattcaa ttcgttaaag
      361 gcttctatcg acgcccgctc aaagtcggtg acaagatgca ggccgggggt ggcggacggg
      421 tgacagaact cgtcgcgcgg cctttgatca atttgttctt cccggaattg tcgggcttga
      481 ttcaaccgtt atcgggtgaa tacgcgggct ggcgcagcgc gctggaacaa gtgcccttct
      541 ttaccggcta cggcgttgag accggcctgc tgatcgatct gcatgaaaag ttcggactgc
      601 gcggcattgc gcaagtcgat ctggaagagc gcgtgcatca caatcagccg ctggacgcgc
      661 tcggccgcat gagttttgcg atcattcaag tcatcgtcgc gcgcctggaa gatcggcaca
      721 agatcaattt gctggcagag atcaatcgga atatgaagat cgtgcggcat gagccgggcc
      781 gttattatct cgatctggaa gagatcggtg atctccagcg cccgccgatg atccagatcg
      841 atgaataccg ccgcgcgcga tccctttcgt gaagatcgct gttgaacctt tgcattccct
      901 aacgtgtagc ttattcaaca atattgtcga ttgactgcca ggagtcttga atgcgtaaat
      961 ttattttgat tctcgcgctc gtggttacgg cctgcgcgcc gatcgccgtg acgcagccga
     1021 tcgtgcccac acccactcga tcgccaattt ccggcccgac gcccacaccc gatccggtct
     1081 gggctgatct ctcgccctat cgcgcggcga tgcgtcctga atttgcacag gatgtcgatc
     1141 agtttgccaa cgcaactcaa tacaagatcg atttaacgat cgcgcccgat ctgaccagtt
     1201 acactggctc tcagcaagtg cactacatta atactgaaac atctgctctg gggcaaatct
     1261 atttccgatt gttccccaac acccaagcgt acggcgggca gctgacgatc acgtcgctca
     1321 aaatgaatgg cgcgtcgatc gaaccgatta tggagctggg caattcggct atgcggatcg
     1381 atctgagttc gccgcttaac ggcggcgctg cggtggattt cgagatgagc tatcaaacga
     1441 tcgtgccgac aacgtccatc acctacggct acgatcaatt cggtttgctc gatcacatct
     1501 tggcgctgcc caatttctat ccgcagattc cggcctatga tgaagaaggc tggaatgtgg
     1561 agattgcgcc cggcgatggc gatgcagtat acagcgacag tgcgttgtat caagtgaata
     1621 tcagcgcgcc gtcgaatcag atcgtcgccg cctcgggcgt atgtgatcga tcgagtaacg
     1681 ccaccaccca aacattaaag tgcgtcagcg gcccgatgcg cgatttcatg atcggcatga
     1741 gtgcagattt ccaaatcgct tctgatcaga tcgacggcat tcaagtcaat tcgtattatc
     1801 tggcgcggga tgccgaggcc gggcaaaacg gtctgaccac cgtcgtgaac tcgatcaaat
     1861 cttatgagca gcgcattggc gcgtatcctt tcaccgaact cgatctatta gagacaccca
     1921 cgcaagccgg cggcatcgaa tatcccggct tgattgtcgt ggccgaagat ctgtatcggc
     1981 aagcgcgcgg ctttcaagaa ggcgccaccg cgcatgaggc ggcgcatcaa tggtggtaca
     2041 gcctggttgg caacgatcag ctcgacgatc cgtggctgga tgaatcgctg acccagttca
     2101 cgacggcgtt atatttctac gatcaatatg gtcaaagtgg cttagacggc gatgtcaatc
     2161 aagacttggt ccgccggcac gatcgcgtca aagggacgga tgaagacaag cgcgccgatc
     2221 taccggtcgc cgcctacact ggtcggcagt acagcgcgat cgtatacggc aaagcgccgt
     2281 tgttttttta tgcgctgtgg cagaaaatgg gcgacgaaaa gtacaacgcc ttcatgcaaa
     2341 actattttca agcgaatcga tatggcgtgg cgaaagtcga tgatctcttg aaggcgatcg
     2401 agacgcagtt ggacaaagcg gcggtcgatg ctttgatgaa gcagtggatt acaacaccat
     2461 aatctccgca ttcccttccc tctcccaaaa taaataggaa aatgccatct tgggagaggg
     2521 tagtaaattc ttctcatttt tcatccttca tcctttgaat gaagtataat tctccgcatg
     2581 aagcagcgtt cctccgctcc cgatctgttc gatcacgccg cgcaagaaac gatcgcgcgc
     2641 agccagccgt tggccgcgcg catgcggccg cgctcgctcg acgaatacgt cgggcaggct
     2701 gagatcatcg gaccgaaccg cttgctgcgc cgcgcgatcg aaacggacaa gatgttctcc
     2761 tcgatcctgc tgtggggtcc gcccggcacc ggcaagacca cgctggccat ggttatc
//
LOCUS       DASRTJ010000518         7109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_354759,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000518 DASRTJ010000000
VERSION     DASRTJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354759"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1255)
                     /locus_tag="VFF70_10460"
     CDS             complement(95..1255)
                     /locus_tag="VFF70_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY45159.1"
                     /translation="MLVEDAVLSNAEEQLLTIGRERGYVTYDEVLEAFPEAETNVDQL
                     DEIFATLTENGVELGDAPEDEGPDEEEISDELDDGTVAAIETDDTVGLYLKEIGRIPL
                     LTAPEEVSLAKRIEKGKFALKRLAKNTLPGKKKFQLEGVLQDAKGAREHLITANSRLV
                     ISVAKKYIGRGVPFLDLIQEGNIGLIRAARKFDYHRGHKFSTYATWWIRQAVTRAIAD
                     QGRTIRVPVHMGDQINKLIRVSHSLTQELGREPNPEELAGAMQVPVKKAEQMIQVARR
                     PISLETPTDEDEDSVLGDFIMDEESIAPIEAVTSNMLREQISDILNSLPPREVRILQL
                     RYGLVDGQSYTLEEVGKKLGVTRERVRQIEAQALSRLRHPTHARKLRDYLHE"
     gene            1916..4198
                     /locus_tag="VFF70_10465"
     CDS             1916..4198
                     /locus_tag="VFF70_10465"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZY45160.1"
                     /translation="MEGLSAFEQVAIYVVLFIALLGLGYALLLRNQILREDKGTAKMQ
                     EVWNAIKDGADSYLRRQLRSILPLIAILTVALFFSVYIVPPSADATEPFRGMDESSVR
                     LVVGLARGVAFIMGAVFSLAVGQIGMRMAVQANVRVASASRRSFGDALRIAYRAGTIT
                     GMLTDGLGLFGGTIIFIIMGKAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGADL
                     VGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSALILGLALTNL
                     TGHLEWIIYPLLVRGIGVVSSIIGTYLVKSGPGKSGDAMKAIFSGFLTSAAISVVLFG
                     IVGFFYMQGVPGSWFRPFAATAAGVVLAILIDRLTEYFTGTHAAPVKNIKSAADTGPA
                     TLILQGLAVGYESSVWSILVIAATLFSSIVIFSGVDAQHEVAYILYGVAMTGIGMLTL
                     TGNNVAMDSYGPISDNANGIGEMAWSGMEDAETKTAQQIMADLDAVGNTTKAITKGVA
                     IGSAVIAAVSLFGSFITDVSKVDPAALANGIRISMPVVFVGFLIGGAVPWLFSSFAIQ
                     AVSRAASLIVLEVRRQFKLGVLDGKVPPDYKQAVGISTTAAQKELIPLALLAVIPPIL
                     VGLFLKVEALGGFLAGIILSGQLLAVFMNNAGGAWDNAKKLIEDEPKNPAKNTGKGSE
                     RHKAGVVGDTVGDPMKDTAGPALNPMIKVVNLVSVIIAPIVVSLKETGIGVIIAGIVL
                     LALLVWAIRSSKRNAPSMSV"
     gene            4295..5335
                     /locus_tag="VFF70_10470"
     CDS             4295..5335
                     /locus_tag="VFF70_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZY45161.1"
                     /translation="MSTAYLFDPIFLEHKQAGHVESPDRLEQINLALDRTGMRDRLIS
                     IAPQPISIERLARVHRPEYIQQVKSIADRGGGGLMARGDETYVAPKSYDAALLASGAV
                     VTGLEAVMRGDVKNAFALVRPPGHHSFADHGEGFCLFNNIAIAAATARAEFKIDRVMV
                     VDFDVHHGHGTNAIFYNDPTVLFFSTHQWGIYPGTGHWREVGAGPGLGYSVNVPMLPG
                     WGDAAMNSVFDELLVPIARRFQPQLILVSAGYDPHWSDRLGSMLMTGHGFFVLTRKLV
                     QLSNEICGGKLVLTLEGGYGLEGLASGVVASFTALLGDEDFIDPIGPPLRSELVIDRE
                     YLKQLCTLHHLD"
     gene            5462..>7109
                     /locus_tag="VFF70_10475"
     CDS             5462..>7109
                     /locus_tag="VFF70_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45162.1"
                     /translation="MTEVALHDYVNEIAGMIEGSSYDAAITHCKHILSQYPKYLEAYR
                     LIGKAALEKEDDATAINVFQRVLAVDPEDFLARVGLSIIFDRGNELDKAIWHMERAYD
                     LVPSNGVIQSELKRLYARRDGVEPDRISLTRGALARMYIQGDLYPEAIADLKRILLEQ
                     PDRIDLQVLLTQVLWRDDRRIEAAAWAQKILTKLPYCLSSNLILGEVWKSSDQQVESE
                     LPLKRAQQIDPENSFAAKFFGDVSPLPNQSVTVERLETPYTTSPSELTGTPEEVPDWL
                     RGLTDNAPVPELDEPEVAKAPKLPTGLHMPDTGPLKSEIPDWLQGLGATNESTVPAVA
                     ADTPFTAEIEPDWLAQMRSGTEPALTTAPAVDELPDVPDWISRLGATAPLAETESTTA
                     TAEPSRSEDMPDWIKQIGADVQEQPSASMIEAAETMTADMPDWLKQPEAKPAESSSIV
                     AEPAAEMPDWLANLKPHDLEAIPSLEKAEPDWLSSLKEEHSLPEFDLSSRSEQAVSTA
                     PEVSEEMPSADDALAFLAKLSAGKEDQLRAQAEQEGTARMD"
BASE COUNT         1514 a         1820 c         1985 g         1790 t
ORIGIN      
        1 ggttttgagg cggccacatc catttccaca acttgacatg tattcaaata aaagatggat
       61 gaggcgggcg cctcatccat cttttatagc gggtctactc gtgcaggtag tcgcgtagct
      121 ttctggcgtg cgttggatgt cgcaatcttg acagcgcctg cgcctcgatc tgccgcacgc
      181 gctcgcgtgt gacgccgagt ttcttgccga cttcttcgag cgtataagat tgtccatcga
      241 ccaggccgta acgcaattgc aagatgcgca cttcgcgcgg tggcaggctg ttaagaatat
      301 ccgaaatctg ctcgcggagc atattactgg tcaccgcttc gatcggcgca atggactctt
      361 catccatgat aaagtcgccc aacaccgaat cttcatcttc gtcggtcggc gtttcgagcg
      421 agattggccg ccgcgcgacc tgaatcattt gctcggcttt cttcaccggc acttgcatcg
      481 caccggccaa ctcttcaggg ttcggttcgc ggccgagttc ttgcgtcagc gaatggctaa
      541 cgcgaatcag cttattgatc tgatccccca tgtgcacggg tacgcgaatg gtgcggcctt
      601 gatcggcgat agcccgggtc accgcctgcc gaatccacca tgtggcatac gtgctgaatt
      661 tgtggccacg atgatagtcg aacttgcggg cggcacggat caagccgatg ttgccttctt
      721 ggatcaaatc gaggaacggc accccccgtc cgatatactt ctttgcgacg gagatcacta
      781 atcgggagtt agcggtgatc aaatgctcgc gcgcgccctt ggcatcttgc agcacgcctt
      841 ccagttggaa cttcttttta ccggggagag tgttcttggc caaacgcttc agcgcgaatt
      901 tgcctttttc aatgcgcttc gccagtgata cttcttccgg cgcggtcaaa agaggaatgc
      961 ggccaatttc tttgagatac aacccgaccg tgtcatcggt ttcgatggcc gcgacagtgc
     1021 cgtcgtcgag ttcgtcgctg atctcttctt cgtccggacc ttcgtcttcc ggcgcatcac
     1081 ccagttcgac accgttctcg gtcagcgtcg caaaaatttc gtcgagctga tcgacgttcg
     1141 tttccgcttc ggggaaggct tcgaggactt cgtcgtaagt gacatagccg cgctcgcggc
     1201 caatagtcaa cagctgctct tcggcattgc tgagcacggc atcttctacc agaattggtt
     1261 ccttggatgg aacttctttg gtatctttga ctttgatctt gactctgctc tttgtttttg
     1321 ctgttttcgc ggccgccaaa tcaacaccca cctctaaaga ttatatccgt tcccacaaat
     1381 aaagagcact ctgtgcgttc atgcctggca cattgtgctc ttgaattgat cgcgcaaacc
     1441 agtcatctaa tatcaagtgt tttaagttct gcttgttcat actacaacaa aggtcgatca
     1501 attgtcaagc acttgtgaag tcagtaattc aatcttaatt cgcatagtta tgtatcgttc
     1561 ctgctagttt tatcgatttt ttatcaagtc tcattcactt cagttcgagg ttcgctaact
     1621 ttgggttgga gctattcccg atcgggccag ctagaccgat cggggtgttg gcgatctcgt
     1681 ccagattact tgatcgggct tagtcgcatc cgatcgatcc cgcgggtctg atcgcttatc
     1741 gcacctaaaa tatagcaaaa ctcgcttgac ttttaggcgg tcattgagta aaatccgcgg
     1801 cggggtttca cgtatcaact gtcattgatc tttatgcgcc tggagttttc gggcgcattt
     1861 ttggcctagc gaccaggccg cgtgcaaaaa taaattacgg aggaagtttc aacatatgga
     1921 aggcttaagt gcattcgagc aagttgcaat ttacgtggtg ttgttcatcg cgctgctggg
     1981 attaggttat gctttactcc tgcgcaatca gattctgcgc gaggataaag gcactgccaa
     2041 gatgcaagaa gtgtggaacg cgattaaaga tggcgcagat tcttacctgc ggcgacaact
     2101 gcgatcgatc ttgccgctga tcgctatcct cacggtggca ttgttcttct cggtttacat
     2161 cgtcccgccg tcggccgatg caactgaacc ttttagaggt atggatgaat cgagcgttcg
     2221 attggtggtt ggtctggcac gcggcgtcgc attcatcatg ggcgcagtct tctcgctagc
     2281 cgtcggtcag atcggtatgc ggatggccgt gcaagcgaac gtgcgtgtgg cttcggcctc
     2341 gcgtcggtcg tttggcgatg cgttgcgaat tgcctatcgc gccggaacga tcacgggcat
     2401 gttgaccgat ggtctggggt tgttcggcgg cacgatcatt ttcatcatca tgggcaaagc
     2461 cgcgccggat gcgctgttgg gcttcggctt cggcggcacg ctgctggcgt tattcatgcg
     2521 cgtgggcggc ggtatcttca ccaaagccgc cgatgtgggc gctgacctcg tcggtaaagt
     2581 cgaagcgggc attcccgaag acgatccgcg caatccggcc gtcatcgccg atctcgtcgg
     2641 cgacaatgtg ggcgactgcg ccggtatggc cgccgacatt ttcgaatcct atgaagtcac
     2701 gatcgtgtcc gcgttaattc ttggtctggc gctgacgaat cttaccggcc atttggaatg
     2761 gatcatttat ccgctgttgg tgcgcggtat cggtgtggtg tcctcgatta tcgggacata
     2821 cttagtgaag agtggccccg gcaagtcagg cgatgcgatg aaggccatat tttcaggctt
     2881 cctcacttcg gcggcgatct cagtggtctt gttcggcatc gtgggtttct tctatatgca
     2941 aggtgtgccg ggcagttggt tccgcccatt cgccgctaca gccgctggcg tggtattggc
     3001 gattttgatc gatcgcttga ctgaatattt taccggcaca catgccgcac cagttaagaa
     3061 catcaaaagc gccgctgata ccggcccggc cactttgatc ttgcaaggtt tggcggttgg
     3121 ctacgaatca tccgtgtggt caatcctcgt gattgcggcg acgctctttt cttcaatcgt
     3181 gatttttagc ggcgtcgatg cacaacatga agtcgcctat attttatatg gcgtagcaat
     3241 gacgggcatt ggcatgttga cgctgaccgg caacaacgtg gcgatggatt cgtacggccc
     3301 gatctccgac aatgcgaatg gtatcggcga aatggcctgg agcggtatgg aagatgcaga
     3361 gactaaaacc gctcagcaga tcatggccga tcttgatgcg gtgggtaaca cgactaaagc
     3421 gatcaccaaa ggcgtggcga tcggatcggc ggtcattgcg gcggtgtcgc tatttggatc
     3481 gttcatcacc gatgtgtcga aagtggatcc ggctgcattg gcgaacggaa ttcgcatcag
     3541 catgccggtt gtgttcgttg gtttcttaat cggcggcgca gtgccatggt tgttctcatc
     3601 attcgccatt caggcggtat cccgtgcggc ttcgctcatc gtattggaag ttcgacgtca
     3661 attcaaactt ggcgtgttgg acggcaaggt cccgccggat tacaagcaag ctgtgggaat
     3721 ttcgacgacc gccgcgcaaa aagaattgat cccgttggcc ttgttggcgg tgattccgcc
     3781 catcctggtt ggcttgttct tgaaggtcga agcgctcggc ggttttctgg caggtatcat
     3841 cctctccggt caactactcg cggtgtttat gaacaatgca ggtggcgctt gggacaatgc
     3901 taagaaattg atcgaagatg agccgaagaa tccggccaag aatacgggca aaggctcgga
     3961 gcggcacaag gccggtgttg ttggcgatac cgttggcgat ccgatgaagg acacagccgg
     4021 tccggcgtta aacccgatga tcaaagtagt gaaccttgtg tccgtgatta ttgcaccgat
     4081 tgttgtatcg ctgaaagaga cgggcatcgg cgtgatcatc gccggtatcg tgctgctggc
     4141 gctcttagtc tgggcgattc gctcatcgaa gcgcaacgct ccatcgatga gtgtgtaagt
     4201 atttgtggag gcaatccagc gggagtgatt gtttaatcac tcccgttttt attttctgct
     4261 tgaattgccg ttcttaatcg agtagaatag gtttatgagc actgcttatc ttttcgatcc
     4321 gattttcttg gagcacaagc aagccggcca tgtcgagtcg cccgaccgct tggagcagat
     4381 caatctcgcg ctcgatcgaa caggcatgcg cgatcggttg atctcgatcg cgccgcaacc
     4441 catctcgatc gaacgcttgg cgcgggtgca tcgacctgaa tacattcagc aggtcaaatc
     4501 gattgctgat cgtggcggtg gtggcttaat ggctcgtggc gatgagactt acgtagcgcc
     4561 aaaatcatat gacgccgcat tgctagcgtc gggcgcagtc gttactgggc ttgaagcggt
     4621 tatgcgcggc gatgtgaaga atgcgtttgc tttggtacga ccacccggcc atcattcttt
     4681 tgccgatcac ggtgaaggct tttgtttatt caataatatt gcgatcgcgg cagcgacggc
     4741 tcgcgccgaa ttcaagatcg atcgagtaat ggtcgttgat tttgatgtgc atcatggcca
     4801 tggaacgaac gcgattttct acaacgatcc aaccgtcctc tttttctcga cgcatcaatg
     4861 gggcatctat cccggcacgg ggcactggcg cgaagttggc gcagggccgg ggcttggcta
     4921 tagcgtgaat gtgccgatgc tgccggggtg gggcgatgcc gcgatgaaca gtgtgttcga
     4981 tgaattgctg gtgcccatcg cgcgccggtt tcaacctcaa ttgattttag tgagcgccgg
     5041 ttacgatccg cattggtccg atcgattggg atcgatgctg atgaccggac acggcttctt
     5101 cgtgctcact cgcaaattgg tgcagctatc caacgaaata tgcggcggca aattggtgct
     5161 gacgctcgaa ggtggctacg gcttggaagg cttggcatcc ggcgtggtcg cttcttttac
     5221 cgcgttgctc ggcgatgagg actttatcga tccgatcggg ccgccacttc gatcggagtt
     5281 ggtaatcgat cgagaatact tgaaacaact gtgcacgctg catcatcttg attgaactga
     5341 atgagcggct gagactggcg aactgccttc ggtcagcggc gttaaaccgg cagttaatcg
     5401 cttgcacaat tcatcgaaac atagcataat cactctcgac agcatttaag gacggagaaa
     5461 tatgaccgaa gtcgcgctcc atgattacgt gaacgaaatt gctggcatga tcgaaggaag
     5521 ttcctacgat gcggcgatca cgcactgcaa gcacatcctc agccaatacc ccaaatacct
     5581 tgaagcctat cgtttaattg gcaaagccgc gttggagaaa gaagacgatg cgacagccat
     5641 caatgtcttt caacgcgtat tggcggttga ccccgaggat tttctcgcgc gtgtcggctt
     5701 gagtattatt tttgatcgag gcaatgaact ggataaagct atttggcata tggagcgtgc
     5761 ttacgatctc gtgccgagca acggcgtaat ccaaagcgaa ctgaaacgct tgtatgctcg
     5821 ccgggatggc gtagagcccg atcggatttc gctgacccgc ggcgcattgg cgcgcatgta
     5881 tatccagggc gatctttatc ctgaagccat tgccgatctt aaacgcatac tgctagagca
     5941 gccggatcgg atcgatctgc aagtcttact gacgcaagtt ttatggcgtg acgatcggcg
     6001 gattgaagca gccgcttggg cacagaagat tctgacgaaa ttgccctact gtttgtcctc
     6061 gaatcttatc ctcggcgaag tttggaaatc aagtgatcag caagttgaaa gcgagttgcc
     6121 attaaagcgc gctcaacaga tcgatccgga gaactcattt gctgcaaagt tctttgggga
     6181 tgtctcgccg ttacccaatc aatctgtaac cgtggagcgg cttgagacgc cgtatacgac
     6241 atcgccaagc gaattgactg gtactccgga agaagttccg gactggttgc gcggactgac
     6301 ggataatgct ccagtgcccg agctagacga acccgaagtc gccaaggcgc ccaagctccc
     6361 gactggcctg catatgcccg acactggtcc gttgaaatca gaaatacctg attggttgca
     6421 agggctggga gcgaccaatg agtcgacggt cccagcggta gcggcggata cacctttcac
     6481 cgctgagata gaacccgatt ggctggctca aatgcgatcc ggtaccgagc ctgcactaac
     6541 aaccgcgccc gccgtcgatg agctccccga tgtgccggac tggatcagtc gattgggtgc
     6601 gactgctcca ttagccgaaa ctgaatcaac tacagcgacc gctgagccat cacgcagtga
     6661 agacatgcca gattggatca agcagatagg cgctgacgtt caagagcagc cctccgcttc
     6721 aatgattgag gcggctgaaa cgatgacggc agatatgccc gattggctca aacagcctga
     6781 ggctaagccc gctgaatcta gctcgatcgt ggcggaacct gcagcagaga tgcccgattg
     6841 gctggcgaat cttaagccgc acgatctgga agcaattcca tctctagaaa aagctgaacc
     6901 cgattggttg agctcgctta aagaagaaca tagcttgccg gaattcgatc tgtcctcacg
     6961 atctgagcag gccgtctcga ccgcgccgga agtttctgaa gaaatgccca gcgccgatga
     7021 tgctttagcc ttcttagcaa aattatctgc tggcaaagaa gatcaattgc gggcgcaagc
     7081 ggagcaagag ggcacggcac gcatggatg
//
LOCUS       DASRTJ010000519         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_260645,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000519 DASRTJ010000000
VERSION     DASRTJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260645"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFF70_10480"
     CDS             <1..379
                     /locus_tag="VFF70_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45163.1"
                     /translation="AKEKAVSLRKTDTLLFITNIVSPGAATLIAALAAAIGGNRMFAQ
                     AATQTEDGGWKLACILVAVFGFIATVTGMLKKQYDDRLAQGNQCVGRLLTLDSNLATG
                     SSSWEADTQEYGEIVKSFSEFVS"
     gene            548..1408
                     /locus_tag="VFF70_10485"
     CDS             548..1408
                     /locus_tag="VFF70_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZY45164.1"
                     /translation="MTTSTLTTSRSIAHLIEPQYAIEGAGVLLRRSIAPQVSNPYDPF
                     LLFDHFAFNDPVEGPIVGFPMHPHRGIETVTYMLEGSVHHRDSLGNAGLIHAGDVQWM
                     TSGRGIMHEEMPRRGPSGLINGFQLWVNLPAAQKMTTPRYQEVNAATIPVVKKNGAAV
                     RLVAGEFDGTRGPVTEIAASPLYLEVNLDPGAAFSIDLPREHTALAYLFEGAGQFGAE
                     QESIEAVKMIVFDHGDRIEVHSDTGARFMLIAGAPFREPIAPYGPFVMNTPEEIRQAI
                     ADLRAGTFVK"
     gene            complement(1465..2379)
                     /locus_tag="VFF70_10490"
     CDS             complement(1465..2379)
                     /locus_tag="VFF70_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45165.1"
                     /translation="MRKLSEIIPFVSKKRIWSIGIFSGDSPFSLIDPPTMKNPVLTAR
                     EVTDVSAEFVADPFMLQVDLTWYMFFEVMNVKTQRGAIGLATSRDSWHWTYRQIVLST
                     DQHLSYPYVFKWQADCYLLPEKAAAGGVQLYRAIDFPTRWELIETILPIGLVDPSIVR
                     YADRWWLFAADHPTGYNALRLFQADKLAGPWSEHPRSPIVRRDLRGARPAGRVITIAD
                     RLYRFAQDNRVSYGRQVFAFEITELSATTYQEKIVSDQPILTASGRGWNKHGMHHLDP
                     HRIAPDQWIACVDGAHKRIAFGKKQRVA"
     gene            2478..3209
                     /locus_tag="VFF70_10495"
     CDS             2478..3209
                     /locus_tag="VFF70_10495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY45166.1"
                     /translation="MIIHRTPTRYFSAIVLAAICLLGLCLPRSIYAARPDAASKPFRL
                     PFADPPGPSSWYLGQGYGNTLGAYFQRDTTYRAGQGLHFGLDFAAPCGTPVIAIGGGV
                     VAKVDAPEHGALPHNLMIQLDAGYAALYGHLLERSKLSVGQRVTQGQVVGLSGDSFGT
                     CHSAPHLHLEIRSPNYLTAYNPINLIDADWNALALVGAFQRGFERDLSHPRQWQYPDE
                     QPDTKFGTPLLNNYADPWPPDWYRN"
     gene            3523..4494
                     /locus_tag="VFF70_10500"
     CDS             3523..4494
                     /locus_tag="VFF70_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45167.1"
                     /translation="MPAPTTEPVARPTSELRQLTTGGCCVNPSWSPDAKQVLFIDKPT
                     ANAPTGLYAIDVTATLAQSPQVIGRVGDYSPDRLLIAYRQSGQTIVENLKTGVSSIIP
                     NDGHEVIFAPDNKHIAWEAEAINGPYDQRPNNIYLANIDGRDAVRITKVYGGGLVGWS
                     PQGLKPVFLGRPSLDVHTRTLTILDLTTNVAVDLVSAERISGVSLSANGAWLAYNITF
                     EADKTRNGIWLERSDGMQMLRIDQWGAYQWRDDSHLLIIPQRTSSDRSFDLIEIDAAS
                     SESRLLIDGSITALNIFNGDWRVSPDGQYIVFLNSTDRNLWLVKLPR"
     gene            4520..5044
                     /locus_tag="VFF70_10505"
     CDS             4520..5044
                     /locus_tag="VFF70_10505"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HZY45168.1"
                     /translation="MKSKISLWITMLLILAACTTTPPAVKPTAIDVAALETPIPDEGH
                     DQVQAGSQITYKHNPPASGPLYSVVIQYGLYETDVPAGFWVHIIERGGIVFLYQCGTE
                     CSALKQKLGDMLDSVPVSKHGNVKLVIVPYSNMDHLITAVAWNVQLPLDQFNLDLLTN
                     FYVRHVDHGVEDVP"
     gene            5054..6007
                     /locus_tag="VFF70_10510"
     CDS             5054..6007
                     /locus_tag="VFF70_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY45169.1"
                     /translation="MTDYIQWLRSNIGHHKTILAYTTAIIRDDRGRVLCQRRRDFRDA
                     WWGLPGGALEIGETFSACAKREAFEETGLRIKPIKLIGLYASPEWDVTYPNGDQVQQF
                     TIALDCRIVAGELKSDSDEVIHQEFFALHAVPSLPPWYAAMLGHLDRREAYFDPPIIG
                     SPVESYMQMIRHFVGASTVLVAGASAAIFDDRQRVLLGLRGDNHLWGMPAGQMELGET
                     PAGTVMREALEEVGLRIRVTQLISVQTGPDAIHAYPDGNQVQVVGARFRAEIIEGDLK
                     PDGTETLAVDWFDVNDLPPTLPRHEKIICLAIDHPEGGQFS"
BASE COUNT         1345 a         1679 c         1661 g         1389 t
ORIGIN      
        1 ggcgaaagaa aaagcagtta gtcttcgcaa gaccgacaca cttttgttca tcaccaatat
       61 cgtgtcacct ggcgctgcca cgctgattgc ggcgctcgcc gccgcaatcg gcggcaatcg
      121 aatgttcgcg caggcagcca ctcaaaccga agacggtggc tggaaactcg cctgtatctt
      181 ggtcgcagtg ttcggcttta tcgccacagt gaccggcatg ctcaagaaac aatacgacga
      241 tcgactcgcg cagggcaatc aatgcgtcgg ccgtttgttg acactcgatt cgaatctcgc
      301 cacgggcagc agcagttggg aagcagatac gcaggaatac ggcgagattg tcaaatcgtt
      361 ttctgaattt gtcagttgat gggtgtgtct gtgtgactct gaaccgcccg tgggatcgat
      421 cgtgtgggcg gttttatttc gaagagcagc cacgacatcc ctatgtcgtg gagatcgagc
      481 agcagcatcc cgatcgatgt gttatgattt ttcaaagtaa ggcccacggg aactctaatc
      541 aataactatg acaacatcaa ctctcacgac ttctcgatcg atcgcgcacc tcatcgagcc
      601 gcagtacgcc atcgaaggcg cgggcgtgct gctgcgtcgc tcgatcgcgc cgcaggtttc
      661 taatccatac gatccattcc tgctgttcga tcatttcgcc ttcaacgatc cggtcgaagg
      721 cccgatcgtc ggctttccca tgcatccgca tcgtggcatc gagaccgtca cgtacatgct
      781 cgaaggttcc gtccatcatc gcgatagtct gggcaacgcc ggtttgattc atgcaggtga
      841 tgtgcagtgg atgaccagtg ggcgcgggat catgcacgaa gaaatgccac ggcgcggtcc
      901 cagcggcctg atcaatggtt tccaattgtg ggtcaatctg cccgccgcgc aaaagatgac
      961 cacgccgcgc tatcaagaag tcaacgccgc cacgattccc gtcgtcaaga aaaacggcgc
     1021 ggcggttcga ttggtcgcgg gtgaattcga tggcacccgc ggccctgtca ccgagatcgc
     1081 ggcctcgccg ttatatttgg aagtcaatct cgatccgggt gcagctttct caatcgatct
     1141 gccgcgcgaa cataccgcgc tcgcttacct cttcgaaggt gcgggtcaat tcggcgctga
     1201 gcaagaatcg atcgaggcgg tgaaaatgat tgtattcgat cacggcgatc gaattgaagt
     1261 ccacagcgat acaggcgcgc gcttcatgct catcgccggt gcgccgttcc gtgaaccgat
     1321 cgcgccgtac ggcccgttcg tcatgaacac gcccgaagaa atccgccagg ccattgctga
     1381 tttacgtgcc gggacatttg taaaataagc cgatcttctc aatatcgact gcccgcgcga
     1441 tcaaatcgtg cgggcagttt atttttaagc cactctctgt ttcttgccaa acgcaattcg
     1501 tttgtgcgca ccatcgacgc acgcgatcca ctgatctggt gcaatacgat gcggatcgag
     1561 atggtgcatg ccgtgtttgt tccagccgcg cccgctggcc gtgaggattg gttgatcgct
     1621 gacgatcttt tcttgataag tcgtagctga aagctcggtg atctcaaacg cgaagacttg
     1681 ccgcccatac gacacgcgat tgtcctgcgc gaaacgatac agccgatcag cgatcgtgat
     1741 cacccggccg gccggtcgag cgccgcgcag atcgcgccga acgatcgggc tgcgcggatg
     1801 ttcgctccac gggccagcca acttatcggc ttgaaacaag cgcagggcat tataaccggt
     1861 cggatggtcg gccgcgaaca gccaccaccg atcggcatac cggacgatcg agggatcgac
     1921 caggccgatc ggcaggatcg tctcgatcaa ttcccagcgc gttggaaaat cgatagcgcg
     1981 atacaactgc acaccgccgg ccgcagcttt ctcgggcagc aaataacaat cagcctgcca
     2041 cttgaacaca tacggatacg ataaatgctg atcggtgctg agcacaatct ggcgatacgt
     2101 ccagtgccag ctgtcccgac tggtggctaa cccgatggcg ccgcgctgcg ttttcacatt
     2161 catcacttca aaaaacatat accaagttaa atccacctga agcataaacg gatcggccac
     2221 gaattcagcg ctcacatcgg tgacctctcg cgctgtcaaa acaggattct tcattgtcgg
     2281 cggatcgatc aaactaaacg gtgaatcgcc gctgaagatg ccgatcgacc aaatgcgttt
     2341 cttgctgacg aacgggatga tctctgatag tttacgcaat tatcttatcc attggtccac
     2401 ccacacactg ccgacattct agcataccgt ttggttttct ctgcgggtcg cagttgtctg
     2461 tgatagaatc atgatcgatg atcatccacc gcactcccac tcgttacttc agcgcaattg
     2521 tgctcgctgc gatatgcctg ctcggcttgt gcttgccccg atcgatctac gccgcccgac
     2581 cggatgcggc ctcgaaaccg tttcgtctgc cttttgcgga tccgcctgga cccagcagct
     2641 ggtatttggg tcaaggctat ggcaacacct taggcgcata ttttcagcgc gataccactt
     2701 atcgcgccgg acaaggtctg cactttggtt tggattttgc cgcgccatgc ggcacacccg
     2761 taatagctat cggcggcggc gtcgtcgcga aagtcgatgc gccggaacac ggcgcgctgc
     2821 cgcacaactt gatgatccaa cttgatgccg gctatgcggc cttgtacggt catctcctcg
     2881 aacgatcgaa gttgtctgtt ggacaacgcg tcacgcaagg ccaagtcgtt ggactgagcg
     2941 gcgattcatt cggcacctgt cattctgcgc cgcatctcca tctcgaaatt cgcagcccaa
     3001 attatttgac cgcctataac ccgatcaatc ttatcgacgc cgattggaat gcgctagccc
     3061 tcgtcggcgc tttccagcgt ggctttgagc gtgacctcag ccatccgcgc caatggcaat
     3121 atcccgatga acagcctgat acaaaatttg gcacaccgct gctgaacaac tacgccgatc
     3181 cgtggccgcc agactggtat cggaattaaa gcaggcaggt tgaagagaaa cagaagcagc
     3241 ctgagcgaag agttgaagtc taagacttca actcgaagtc gaaggagcga tttggcatcg
     3301 aacgagagat agcgagatga caagatagca agaagaatca gcgggttgac tgcatcaaca
     3361 ctcatgaact caatacgaat gctaaaaata ttttttctct ttatcctatt agccgcttgt
     3421 tctcctgcgc ctgggccgat ccaaacgccg ccagtcgcaa catcgggctc accggatctg
     3481 cgccctccgc tttccggcgg gttgagtaat ccaccgacta tcgtgcccgc gccaactacc
     3541 gaaccggttg ctagaccgac atctgaattg cgacaactca ccacgggcgg ttgctgcgtc
     3601 aatccgtctt ggtcgcccga tgcaaaacaa gttctcttca ttgataagcc caccgccaat
     3661 gcaccgaccg gcctctacgc gattgatgtg acggccacgc tcgctcaatc gccgcaggtg
     3721 atcgggcggg tgggcgatta ctcgccggat cgattgttga tcgcctatcg tcaatccggc
     3781 cagacgatcg tcgaaaatct gaagacgggt gtcagttcga tcattccgaa cgatgggcat
     3841 gaagtgatct tcgcgccgga taacaaacac attgcgtggg aagcggaggc gatcaacggc
     3901 ccgtatgatc aacgcccgaa caacatctac ttggcaaaca tcgatggccg tgatgccgtg
     3961 cgcattacca aagtgtacgg cggtggcttg gtcggttggt cgccgcaggg cttgaagccc
     4021 gtatttttag ggcggccttc attagatgtc cacacgcgca ccctcactat cctcgatcta
     4081 acgaccaacg tcgcagtcga tctcgtcagt gctgaacgta tcagcggcgt cagcctgtcc
     4141 gctaacggag cgtggctcgc ctacaacatt accttcgagg cagacaaaac gcgtaacgga
     4201 atctggcttg agcgatccga cggcatgcaa atgctgcgga tcgatcagtg gggcgcgtat
     4261 caatggcgcg atgattcgca tctgctgatc attccgcagc gcacgtcctc tgatcgatct
     4321 ttcgacctga ttgaaatcga tgcagcttcc agcgagagcc ggctactgat tgacggttcg
     4381 atcacggcgt tgaatatttt caacggcgat tggcgcgttt cgcccgacgg gcagtatatt
     4441 gtgtttctca atagcaccga tcgtaattta tggcttgtca agttgccaag ataattgaag
     4501 tgcgtgacag aggtctcgca tgaaatctaa aatctcgctc tggatcacga tgctgctcat
     4561 tttagcggcg tgtacgacga ctccgccggc tgttaaaccc acagcgattg atgttgcggc
     4621 gcttgagacg ccgatcccgg atgaaggcca cgaccaagtt caagccggca gccagatcac
     4681 ctataaacac aatccgcccg cgtccgggcc gctgtattcc gttgtgattc aatacgggct
     4741 atatgaaaca gatgtgccgg caggtttttg ggtgcatatt atcgaacggg gcggcatcgt
     4801 ctttctgtat caatgcggca cggagtgcag cgcgctgaaa cagaaactcg gcgatatgct
     4861 ggatagtgtg ccggtgagca aacacggcaa tgtgaaactg gtgattgtgc cttattcgaa
     4921 tatggatcat ctgatcacgg ccgtagcctg gaatgtgcag ctgccgctcg atcagttcaa
     4981 tctggatttg ctgactaact tctatgtgcg gcatgtcgat cacggagttg aagatgtgcc
     5041 gtagcgatcc agtatgaccg attatattca atggcttcgc agcaacatcg gtcatcacaa
     5101 aacgatcttg gcttatacga ccgcgatcat tcgggatgat cgcggtcgag tgttgtgtca
     5161 gcgccgccgc gattttcgtg atgcatggtg gggtttgccc ggcggcgcac tggagatcgg
     5221 ggagacgttc agcgcatgtg cgaagcgtga agcgtttgaa gaaacaggct tgcgcatcaa
     5281 gccgatcaag ctgatcgggt tgtatgcttc gcctgaatgg gatgtgactt atccgaatgg
     5341 cgatcaggtt cagcaattta cgatcgcttt agattgccgc atcgtcgcgg gagaattgaa
     5401 gtccgacagc gatgaagtga ttcatcaaga atttttcgcg ttgcacgctg tgccatcgct
     5461 gccgccgtgg tacgcggcga tgcttggtca cttggatcga cgcgaagcct acttcgatcc
     5521 gccaatcatc ggatcgccgg ttgagagtta catgcagatg attcggcact ttgttggcgc
     5581 atcgacagtt ttggtggcgg gcgctagtgc ggctatcttt gatgatcgtc agcgtgtgtt
     5641 gctgggcttg cgaggcgaca atcatttgtg gggcatgcct gccggacaaa tggaattggg
     5701 tgagacgccc gcgggcaccg tcatgcgtga ggcgcttgaa gaagtcgggt tgcgtattcg
     5761 cgtgacacaa ttgattagtg tgcagaccgg gccggatgcg attcacgctt atcccgatgg
     5821 caatcaagtg caggtcgtcg gtgcgcgctt ccgcgccgag atcattgagg gtgatctcaa
     5881 gccagatggc acggaaaccc tcgcggtcga ttggttcgat gtgaatgatc tgccgcccac
     5941 cttgccgcgc catgaaaaga tcatctgtct ggcgatcgat catcccgaag gcggacagtt
     6001 ttcatagggg cgcggtcacc tcgccccacc attttacggt gtataagccc acagatcatt
     6061 caggggctta gtat
//
LOCUS       DASRTJ010000520         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_165612,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000520 DASRTJ010000000
VERSION     DASRTJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165612"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2604
                     /locus_tag="VFF70_10515"
     CDS             <1..>2604
                     /locus_tag="VFF70_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45170.1"
                     /translation="SVAYSYGLSVAGLYLVFALAARSRGIYRIQIVAVLMAGLVPGVT
                     NVLHLVGILTVDLTPFSFALSGVLLAWAIFRHRLLDLVPIAQEAMIEAMTDGMLVIDE
                     QNRIITLNSAAERIFDVVAPRLVGQPIDRISSHWPTLQPADKSWLEVQDEITVNGFDY
                     ELRSSPLNNRQGRSIGYLLTLHDITHRKQVEEQLKVQQQLLENLLAVAHATSLHSDLQ
                     QTLYDILEVAESLTTAERGSLFLLDASGNVVDAKEMRDGAPSDKRRHVLTEVLNKGLL
                     GWVAAHHATAIIDDTLSDDRWLHLTDESSPIRSVLAVPIVRGPILLGALFLLHSQPGH
                     FTRENAQFIQAAVAQMSLALQQAQAYEAQRRMADRQTTLYEVLRAVGGQLDPESVARL
                     AVAQIERLTGWRNIVVATLRADQKTLEIRGAGGALATALGLVIENGWGIMGRAAATGR
                     TQCVSDVRRDADYVEADPATRSELAVLLRGGNRIRGVLNIESHQLAAFDTYDISLAES
                     LADAIGLALDSAELYREISEEHSRLQAMINSSRDGIALLSVERYFLIGSARMNELLGL
                     KGAPNDLIGRSLFDVLEEIRPAAPKAVEVIIDVTRRVQETNEPSAEAEFEIPPRTVKW
                     ITLPVLMNDTLLGRLLIIRDITGDRELEQARDDLTHMLVHDLRNPLGVILSAFELLTM
                     ARSQTLAEHQREVITLGQQATHRVMDLVNAILDVSRFESGQMPVNREPTELKQLIEDA
                     LQLQSVLANQKQIQLSRVLPEDLPLALIDRDLMSRVIQNLVSNAIKFTPEGGAISVSV
                     ELDTTDRSNLRVSISDTGPGISPEIQGRLFQKFVAGQVKGQGSGLGLAFSRLAVEAHG
                     GHIWVDSQIDHG"
BASE COUNT          568 a          673 c          771 g          592 t
ORIGIN      
        1 agcgtggcct actcgtacgg cctatctgtg gcgggcctat atcttgtctt cgcactggca
       61 gctcgatcac gtggcattta tcggattcaa atcgtggcgg tgttgatggc cggcctggtg
      121 ccgggcgtga cgaatgtctt acatctagtc ggcatactga ctgtcgatct aactccgttt
      181 agcttcgccc tcagcggtgt gttgttggcg tgggcgattt ttcgtcaccg cttgctcgat
      241 ctggtgccca tcgctcaaga ggccatgatc gaagcgatga cggatggcat gttggtcatc
      301 gatgagcaga accgcatcat cacgttgaat tcggctgccg aacgcatctt cgatgttgtc
      361 gcacccaggc tggtcggaca accgatcgat cggatcagct ctcactggcc gactttgcag
      421 ccagcggata agagttggct cgaggtacag gatgagatca ctgtcaacgg gtttgattat
      481 gaattgcgta gttcaccgtt gaacaatcgc cagggccgat cgatcggata cctgctgact
      541 ttgcatgaca tcacccatcg caaacaagtc gaagagcagc tcaaagtcca gcagcaatta
      601 cttgaaaatc ttctggccgt ggcgcacgcc accagcttac attcggactt gcaacaaacc
      661 ctgtacgaca tacttgaagt ggctgagtca ctcaccaccg ccgagcgggg cagcctcttc
      721 ctcctcgacg cgtccggtaa cgtcgtggat gccaaagaaa tgcgtgacgg cgcgccttcg
      781 gacaagcggc gacacgtcct taccgaagtg ctaaacaaag gactattagg ttgggttgcg
      841 gctcatcatg caacagcgat catcgacgac accctcagcg acgatcggtg gctgcacttg
      901 accgatgagt cgagccccat ccgatcggtc ctggcggtcc cgatcgtgcg gggtccaatt
      961 ttattgggcg cactctttct gctccattcc caacccggtc actttacccg ggaaaacgcg
     1021 cagttcattc aagcggcggt cgctcaaatg tctttggcgc tgcagcaggc gcaagcctac
     1081 gaagcgcaac gccgcatggc ggatcgtcag acgacgttgt acgaagtgct gcgcgcggtc
     1141 ggtggccagc tcgaccctga gagtgttgcg cgtctcgctg tggcgcaaat cgagcggctg
     1201 acgggctggc gaaatattgt ggttgcgacc ctgcgcgcag atcagaaaac tttggaaatt
     1261 cgtggcgcag gcggcgcgct ggccactgcc ttaggcctgg tcattgagaa cgggtggggt
     1321 atcatggggc gcgcggctgc cacgggacga actcaatgcg tctcagacgt caggcgggac
     1381 gctgactacg tcgaagcgga tccagccacc cgcagtgaac tggcggttct gttacgcggc
     1441 ggcaatcgga ttcgaggtgt gctgaatatc gagagtcatc aattggccgc gtttgatact
     1501 tatgatattt cattggccga atcgctggcg gatgcaatcg gcttggcgct ggatagtgct
     1561 gagctgtatc gggaaatatc ggaagagcat agccgcttgc aggcaatgat taattccagc
     1621 cgtgacggga ttgcgttgtt aagtgtcgag cgatacttct taatcggcag cgcgcggatg
     1681 aatgaactgc tcggcttgaa gggtgcgccg aatgatttga tcggccgatc gcttttcgac
     1741 gtgttggaag aaatacggcc tgcggcaccg aaggctgtgg aagtcatcat tgatgtgacc
     1801 cgccgcgttc aagaaacaaa tgagccatct gcagaagccg agtttgaaat tccgcctcgc
     1861 acagtaaagt ggatcacact gccggtattg atgaatgaca cgctgctggg tcgtttactc
     1921 attatccggg atatcactgg cgaccgagaa cttgagcaag cgcgtgatga tttgacgcac
     1981 atgctggtac acgatctgcg gaatccgctg ggcgtgattt tgagcgcatt tgaactatta
     2041 acgatggctc gatcgcagac gttggctgaa caccagcgcg aagtcatcac cttaggacag
     2101 caggccacgc atcgcgtgat ggatttagtc aacgcgatcc tggacgtgag ccggtttgaa
     2161 agtggccaaa tgcctgtgaa ccgcgagcca acggaattga aacaattgat cgaggacgcg
     2221 ttgcaactgc aatcagtgtt ggccaaccag aagcagattc aattgtcccg agtcctgccc
     2281 gaggatttgc ccttagcgct cattgaccgc gatttaatga gtcgagtcat acagaatctt
     2341 gtgagcaatg cgatcaagtt taccccagaa ggcggcgcga tctccgtctc tgtggagttg
     2401 gatacgaccg atcgatccaa cctgcgcgta tcgatcagtg atacgggtcc gggtatttca
     2461 cctgaaattc aaggacgatt atttcagaaa tttgtggcgg gccaagtgaa gggccagggc
     2521 agcggcctgg gtctggcttt tagtcgcctg gcggttgagg cgcacggcgg acatatttgg
     2581 gtggatagtc agatcgatca tggg
//
LOCUS       DASRTJ010000521         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_71320,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000521 DASRTJ010000000
VERSION     DASRTJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71320"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1316)
                     /locus_tag="VFF70_10520"
     CDS             complement(36..1316)
                     /locus_tag="VFF70_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45171.1"
                     /translation="MKSFHRTLLISLLLSILIAPAVPVELAAAQSGEPTIYVVQAGDT
                     LYSIAQRYGIDVNALAETNGLINPDQIYVGQSLTIPSPSAASTSTGPSVNRIHAVQKG
                     ETLFSIAVTLGTSIRAIAEANHFSNTNWVLIGQQLVIPISTKSPTLAQPSTPLPAPFI
                     SIDIGPLPLQQGSLLVITVRTVKPVTLSGTFYSQTIPFAIDNEKYIGLAGAGASPVTG
                     VKPGIYPVSVSAVDNNGTQTTVTTNVQINAGHFNFEYINLPPDRQDLLDPTLLAYERD
                     KLNAVWTIFNPTRYWHGLFNVPVDHYTRISSPFGTRRSYDGGPFTSYHEGTDFAIPAG
                     TPVYAPADGVVVLAELLTVRGNAIVIDHGWGVFSGLYHLQSFNVTVGQVVHQGDLVAL
                     SDNTGLSTGAHLHWDIRIRGLNVNALFFTQQVYP"
     gene            complement(1436..1789)
                     /locus_tag="VFF70_10525"
     CDS             complement(1436..1789)
                     /locus_tag="VFF70_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZY45172.1"
                     /translation="MRAISSTGEVLLQRIKWCDTFGSKLRGLMFRRSIDPAEGLVLVE
                     TRSSIAATAIHMFFVPFDIAAIWLDDQFQVVHTALAIAWHPYYASPKAACYVLEASPE
                     LLQRVKIGEHLKFEK"
     gene            1947..4106
                     /gene="glgP"
                     /locus_tag="VFF70_10530"
     CDS             1947..4106
                     /gene="glgP"
                     /locus_tag="VFF70_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZY45173.1"
                     /translation="MIHTDSVLPTFSLPKSIARLGELAYNLWWSWHPEALELFELIDL
                     VLWEQCSHNPVKFLKHVARKNLNAVAQENSYLESYEHVLAAFDQYMHAQSTWFLTQYP
                     DLKDRPVAYFSTEFGLHEALPMYAGGLGVLSGDHCKAASDLGLPLVAVGFMYRQGYFR
                     QRITEDGWQEAINENLNLDESPILRVQDNEGQAVIVHVEMPGRVVYAQLWKVQIGRVS
                     LFLIDTDVDPNEAIDRELTTRLYYSDLERRLQQEIVLGIGGVRALRSLGYNPAVWHMN
                     EGHSAFSSLERMSEIVSNGETFDRALEQIRASTIFTTHTPVPAGHDMFPTWMIEKYFS
                     NYWAGLGLDRDRFLAVAAQHESWGDVFSMTLLALHASERVNGVSELHGEISRKMFAHL
                     WPDRSVDEVPIRSITNGVHTATWLAHRMSDLYDRYLDPDWRDRIDDQAMWNKVRNIPD
                     NALWQVRRHLMRQLVAFARERARAQWQRGGTHPIQIAAGGVLLDPYALTIGFARRFAT
                     YKRAALILRDTDRLFRLVNDPARPVQFIFAGKAHPADDPGKHVIQDLYRIIKDSRWGG
                     RIVFLEDYDMNVARYLVRGVDVWLNTPRRPLEASGTSGEKAAVNGVLNFSMLDGWWRE
                     GYNGENGWAIGDDREYPDTETQDAADAASLFETLEKSIVPLYYDRDSQTHLPLGWLRM
                     MKESIRSLAPMFSTSRMLKEYTAEMYAPAARGEPVNA"
     gene            4396..5493
                     /locus_tag="VFF70_10535"
     CDS             4396..5493
                     /locus_tag="VFF70_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZY45174.1"
                     /translation="MARKIKQRSTMKEVAERAGVAKTTVSHAISGKRPVAPDTREKIF
                     AAMRDLHFSPNPIARRLAGSASHAIALVFPLASQSLAGVELRYLNSIGEVVNRGDYTF
                     LTLTSPRVRVDDLRQVIYSGMIDGVILMRIHVDDERVELLKEAGLPFVMVGRTRHNEG
                     LIYVDLDVEAAIDLAVSHLVELGHMHIAFICPDDLAFGFAYRLMQGYEKSCRKHHLAQ
                     ITIPAGWSDDAGYQAMRMLLSEHPELTGVISWSDVVAVGAISALHASGREIPRDMSLI
                     SFDRSEHLPMASAELTIVDTRPEAIGTQAARMLLNILNEESPRPDQVLVPSRIVQGRS
                     TARPARVPRRSIGATKHLKANGRKEVAASTR"
     gene            5604..6914
                     /locus_tag="VFF70_10540"
     CDS             5604..6914
                     /locus_tag="VFF70_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZY45175.1"
                     /translation="MKTRFSFVSLLAVLGMLLAACQPAATPAPTTAPVPTTVAAKPTA
                     APVVPTAAPVKLVMWTKEGGNQLDEVKAVVADFVKANPNISVDVVNYDVEKLRENFQT
                     SALAGTGPDMLWTVNDHAGVFALADLVQPVDGLGVDTAQFVQAAYDATLVDNQHYGIP
                     LSYGNNLMLLVNKKLIAKVPADTDEMVASAKDFMAKNPGSVGLAWNFGEPFWLVPWLG
                     GENGAVFADAGKTPTLNTPQMVAALTLLAKFKNTDKITPNDCDYACADTLFKDGKAAM
                     IINGDWSLGDYTNVTATKTIDLAISAFPKIVGADVPKPYTSGTYVMFPKDLAGDKLAA
                     ALKLTQFFVSKDVQLKWIKDQKRLPSLQALYTDPAITSDPILSQAAAALVAAGVGMPP
                     FATMRCNWDSMKPNLQAVMAGSMTPADAAAASQKASESCIAQFK"
BASE COUNT         1601 a         1784 c         2023 g         1618 t
ORIGIN      
        1 ggtgcggcgc gcttcgaaag ggcgtcgcac atttgctacg gatacacttg ttgagtaaag
       61 aacagcgcat tcacattcaa gccgcgaata cgaatgtccc aatgcaaatg cgcgcccgtc
      121 gataagccgg tgttatccga cagcgcgaca agatcgcctt gatgcaccac ttggccgaca
      181 gtcacattga aactttgcaa gtgatacagt ccgctgaaca ctccccagcc atgatcgatc
      241 acgatcgcat tcccccgcac ggtcagcagc tcggccagca ccaccactcc atcggcaggc
      301 gcgtagacgg gggtgccggc aggaatcgca aagtcggtgc cttcgtgata actggtgaat
      361 ggtccgccgt cgtacgatcg acgtgtgcca aacggcgaac tgatcctcgt gtaatgatcg
      421 accggcacat tgaacaagcc atgccagtaa cgcgtgggat taaaaatggt ccagaccgca
      481 ttcaacttat cgcgctcata cgcgagcaaa gtcggatcga gcaaatcttg tcgatcgggc
      541 ggcagattga tgtactcaaa gttgaaatga cctgcattaa tctgcacatt cgtcgtgacc
      601 gttgtctgcg tgccgttgtt atcgactgcg ctgacgctga ccggatagat acccggcttg
      661 accccagtca ccggacttgc gccagcgccc gctaagccaa tgtatttttc gttatcgatt
      721 gcgaacggga tcgtttgact gtaaaacgta ccgctcaatg taactggctt gactgtccgc
      781 actgtgatga ccaacaggct gccctgctgt aagggcagcg gtccaatatc gatgctgatg
      841 aatggtgcgg gcaggggcgt cgatggttgg gccagcgttg gtgatttggt gctgatcgga
      901 atgacgagtt gttgtccgat caaaacccaa ttggtatttg aaaagtgatt ggcttccgca
      961 atcgcgcgta tcgacgtacc gagtgtgacc gcaatgctga acagcgtctc acctttttgg
     1021 accgcatgaa tgcgattgac gctcggaccg gttgaagttg aggcggctga gggcgatgga
     1081 atggttagcg attgaccgac atagatctga tcgggattga tcagtccgtt ggtttccgcg
     1141 agcgcattca catcgatccc atagcgctgc gcgatggagt acagcgtgtc gcccgcttgc
     1201 acaacgtaga tcgtcggctc acccgattga gctgcggcca gctcgacggg taccgctggc
     1261 gcgatcaaga ttgagaggag taggctgatc aggagagttc gatggaatga tttcatttgt
     1321 tgttaaacgc gtcattctga gcgaagcgaa gaatctctac cattggttaa cacagttgtt
     1381 gatcgtcata gagattcttc ggcgctgcgc gccccagaat gacttgctaa cgtgttcatt
     1441 tttcaaactt caagtgctcg ccaatcttga cgcgctgcaa taactccggc gatgcttcta
     1501 aaacatagca ggcagctttg ggagaagcgt aatacggatg ccaggctatg gctaaggctg
     1561 tatgcaccac ttgaaattga tcgtccaacc aaatggcggc aatatcgaac gggacgaaga
     1621 acatatggat cgctgtcgcg gcgatgctcg atcgggtttc aactaagacc aagccttcag
     1681 ccggatcgat cgatcgacgg aacatcaagc cgcgcaattt cgatccaaac gtatcgcacc
     1741 acttgatgcg ttgaagcagt acctcgccgg ttgaactaat tgcccgcatg ttgcgattat
     1801 acatgaagat aagggatgag ggaggacgga tgagggctga agaagaatta gatcagaaat
     1861 cataaatcgg agatcggaga ttcacttgac ctctcttcac aactggacta gaatgtccga
     1921 ctgatttcat aaaaggacag gtgcacttga ttcacactga ttcggttctt ccgactttct
     1981 cgctgcccaa atcgatcgcg cgcttgggcg aattggctta caacttatgg tggtcgtggc
     2041 atcccgaggc gctggagctc tttgagttga tcgatctcgt tttgtgggag cagtgttcac
     2101 acaatccggt caagttcttg aaacacgtgg ctcgcaagaa cctgaatgcg gtagcacaag
     2161 aaaacagtta tttggaaagc tacgagcatg tactggccgc ttttgatcaa tatatgcacg
     2221 cgcagtccac ttggttcctc acgcagtatc ctgatttgaa agatcgcccc gtcgcttatt
     2281 tttcaactga atttggtttg catgaagcgc tgccgatgta tgcgggcggt ctcggcgtat
     2341 tgagcggcga tcattgcaaa gcagccagtg atctcggttt gcccttagtc gcggttggct
     2401 tcatgtatcg acaaggctac ttccgtcagc gtatcactga agacggctgg caggaagcga
     2461 ttaacgagaa tttgaatctc gatgagtcgc cgatcttgcg ggtgcaggac aatgagggac
     2521 aagcggttat cgtgcatgtc gaaatgccgg gccgcgtggt ttacgctcaa ttgtggaaag
     2581 tgcagattgg ccgcgtgtca ctgtttttga tcgataccga tgttgatccg aacgaagcga
     2641 tcgatcggga gttgaccacc cgtttgtatt acagcgatct cgaacggcgc ttgcaacagg
     2701 aaattgtgct gggtatcggt ggggtgcgcg cactccgcag tctgggttac aaccctgcgg
     2761 tgtggcacat gaatgaaggc cattctgcct tctccagcct cgaacgaatg agtgagatcg
     2821 tcagcaacgg cgagacattc gatcgagcct tggagcagat tcgcgcttcg acgatcttca
     2881 cgacccacac gccagtgccc gccgggcacg atatgttccc gacctggatg atcgaaaaat
     2941 acttttccaa ttattgggcc ggtcttggcc tcgatcgcga tcggttctta gcggttgccg
     3001 ctcagcatga gtcctggggc gatgtgttca gcatgacctt gctggcactg cacgcttccg
     3061 agcgtgtcaa tggtgtgagc gaactgcacg gcgaaatctc gcgcaaaatg tttgcgcatc
     3121 tctggccgga tcgatcggtg gatgaagtcc cgatcagatc gatcacgaat ggcgtgcaca
     3181 cggcgacctg gctggcgcat cgcatgagcg atctgtacga tcgatatctt gatcccgatt
     3241 ggcgcgaccg catcgacgat caagccatgt ggaacaaagt ccgcaacatt cccgataacg
     3301 ctttgtggca agtgcgccgt catctgatgc ggcagttagt ggcgtttgcg cgtgaacgcg
     3361 cgcgtgccca gtggcagcgc ggcggaacac atccaatcca gattgcggcg ggcggcgtgc
     3421 tgctcgatcc gtatgcgttg acgatcggtt ttgcgcggcg ctttgcgacg tataagcgcg
     3481 cggccctaat actgcgggat accgatcggt tgttccggct cgtgaatgat ccagcgcggc
     3541 ccgtgcaatt tattttcgcg ggcaaagcac acccggccga tgatccgggc aagcatgtca
     3601 ttcaagattt atatcgcatc atcaaagact cgcgctgggg cggacgcatt gtgtttttgg
     3661 aggactatga catgaacgtg gcgcgttacc tagtgcgcgg cgtcgatgtg tggctgaata
     3721 caccgcgccg accgctggaa gccagtggca ccagcggcga aaaggccgca gtgaatggcg
     3781 tcctgaattt ttccatgctc gatggttggt ggcgcgaagg ctataacggt gaaaatggtt
     3841 gggctatcgg cgacgatcga gaatatccgg ataccgaaac gcaggacgcg gctgatgcag
     3901 cctcattgtt cgagacgctg gaaaaatcga tcgtgccgtt gtattacgat cgagatagcc
     3961 aaacccatct gccgttaggc tggctgcgga tgatgaagga atcgatccga tcgcttgcgc
     4021 cgatgttcag cacttcgcgc atgttgaaag aatatacagc tgaaatgtat gcgcccgccg
     4081 cgcgcggtga gccggtgaac gcgtaaagcc gttgagtgag ttgaagcgct caaattaatt
     4141 tcatagagat tgcacaaact cgccgatcga ggtcaagtac ctcgaccggc gagtttttgt
     4201 cagggttatg ctcaacggcc aggcatgttc agcaaaattt gcctaaggta gcgcaaacaa
     4261 ttcgcagcgg tttgcgtgag aaggctgaaa attgatcgat taggctattg acgcgcaaac
     4321 cggtttgtgt taaagtagcc tgtgtgccgc gttgtgcggc atctgtcacg ttgagggcat
     4381 ttaagcaggg cagcaatggc gcgtaagata aagcagcgat cgacaatgaa agaagtggcc
     4441 gagcgggccg gtgtcgccaa gacgaccgtc tcgcatgcca tcagcggcaa gcggccggtg
     4501 gcgcccgaca cccgcgaaaa aatcttcgca gccatgcgcg atctgcattt tagccccaac
     4561 cccatcgcgc ggcggctagc tggcagcgcc agtcacgcta tcgctctggt ttttcctctc
     4621 gcctcacaat cattggcggg agttgaactg cgttatctca atagcatcgg cgaagtggtt
     4681 aatcgtggcg actatacttt tctgactttg acttcgccgc gggtgcgcgt cgacgatctg
     4741 cggcaggtga tatattccgg catgatcgac ggcgtcatct tgatgcgcat tcacgtcgat
     4801 gatgagcgcg tcgaactttt gaaagaagcc gggctgccgt ttgtaatggt cggacgcacg
     4861 cgtcacaacg aaggcttgat ctacgtcgat ctggatgtgg aagccgccat cgatttggcg
     4921 gtcagtcatc tggtggaatt gggtcacatg cacatcgcgt tcatctgccc ggacgattta
     4981 gcgtttggtt ttgcgtaccg tttgatgcag ggttatgaga agagctgccg caaacatcat
     5041 ctagcgcaga ttacgatccc ggccggttgg tcggatgacg cgggctatca ggccatgcga
     5101 atgttactga gcgagcatcc cgaattgacc ggtgttatca gctggtcaga tgtggtggcg
     5161 gtcggagcga tcagcgcgct gcacgctagt gggcgtgaga ttccgcgcga tatgtcgttg
     5221 atctcgttcg atcgatcaga gcatctgccg atggcttctg ctgaactcac gattgttgat
     5281 acgcgccccg aagcgatcgg aacacaagcg gcgcggatgt tgctcaatat tttgaacgaa
     5341 gaaagcccac gtcctgatca agttttggtg ccgtcacgca tagttcaagg tcgcagcacg
     5401 gcacgacccg caagggtacc gcgaagatcg atcggggcca ctaagcactt gaaagccaat
     5461 ggacgaaagg aggtggcggc cagcactcga taaatagtca gattcattca cgccgtagat
     5521 tcaatcgaca cttgttcgag cgaaatccga tcgaacaagc acatccaata atgattacaa
     5581 atactcctgg aggagcaaga aaaatgaaaa cccgtttctc gttcgttagt ttgttagccg
     5641 tactgggcat gctgttggct gcttgccaac cggcggccac acctgcgccg accaccgccc
     5701 cagttccgac gacagtagcg gctaagccaa ctgccgcgcc ggttgtaccg acggctgcgc
     5761 ccgttaaatt agtcatgtgg actaaagaag gcggcaatca gttggatgaa gtgaaggctg
     5821 tcgtcgccga ctttgtgaag gcgaatccga atatcagcgt cgatgtggtc aactatgacg
     5881 ttgagaagtt gcgcgagaat ttccaaacct cggcgctggc cggcaccggt ccagatatgt
     5941 tgtggacggt gaatgaccac gcgggtgtgt tcgcgctggc cgatctggtt caacccgttg
     6001 atggcttggg cgttgacacc gcgcagttcg tgcaggccgc ctacgatgcg accctagttg
     6061 ataatcagca ctatggcatt ccgctgtcct atggcaacaa cctgatgttg ctcgtcaaca
     6121 agaagctcat tgctaaagtt cccgccgata ccgatgagat ggtggcctca gccaaggatt
     6181 tcatggcgaa gaaccccggc tcagtcggtt tagcctggaa cttcggtgag ccgttctggt
     6241 tggtgccgtg gctgggtggt gaaaacggcg cagtcttcgc agatgcgggc aagaccccaa
     6301 ccttgaatac gccgcaaatg gtagccgctt tgacgctgtt ggctaagttc aagaacaccg
     6361 acaagatcac gccgaatgat tgcgattatg cctgcgccga tacgctgttc aaagacggca
     6421 aggctgcaat gattatcaat ggcgactggt cgttaggcga ttacacgaat gtaactgcca
     6481 ccaagacgat tgatctcgcg atctcggcgt tcccgaagat cgtcggtgcc gatgtcccca
     6541 agccatacac ttcgggcacg tatgtcatgt tcccgaaaga cctggcgggc gacaagttgg
     6601 cagccgcttt gaagttaacc caattcttcg tctcgaagga cgttcagttg aagtggatca
     6661 aggatcaaaa gcgattgccg tcattgcaag cgctgtatac tgatccggcg atcaccagcg
     6721 atcccatcct gtcgcaggct gcggctgcgt tagtggctgc gggtgtgggt atgccgccgt
     6781 tcgcaaccat gcgctgcaac tgggattcga tgaagccgaa cttgcaagcc gttatggcgg
     6841 gcagtatgac gccggcagat gccgcggctg cttcacagaa agcgtccgag tcctgcatcg
     6901 cgcagttcaa gtaaatcgag ttgtgtctta aaactagagg ggataggctg cctatcccct
     6961 ctagcctaat agggtgtatc atcaaggatg ttgatcggta gtggtaagaa gctttctcgg
     7021 tgaagt
//
LOCUS       DASRTJ010000522         7670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_33202,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000522 DASRTJ010000000
VERSION     DASRTJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33202"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VFF70_10545"
     CDS             complement(<1..1489)
                     /locus_tag="VFF70_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZY45176.1"
                     /translation="MPNTAIIDLTTSRIEIDRTPDRLIRAYLGGRGLNMHYLRKYLNV
                     AGDPKSIDPLGPDNPLIFGSGLLTGTIAPNAARFNVSAKSPETLGLGDANCGGFFAAA
                     MRKAGFDRLIIVGKCDRPSYLLLEAGRISIHAADEIWGTTSPKAQEMLKAKHGGSTVS
                     AVIGPAGENLVRMASIMTGLKNTAGRCGMGAVMGSKNLKAIVATGGGSIPVDDKEMLR
                     ATRLEQTKYLQGSKVIQTLGKLGTPLLYEVSNRLGAIRTRNSQDNFFADTLNATEIEK
                     FSDKMLACTSCVVHCRHRNTLGGEGPEYSTIGLLGANIGLAPTDQVIMLNNLVNDLGL
                     DASSVGTIIAWAMELYQRGLITADMTDGPLDWGDYERAYELINDIAYRRGFGNILAES
                     TQAKKFFPLEAADYLIAVKDLPQSDPHDVRYIKSFALGIAVASRGADHLRNRPTLDIL
                     RLPDDVRMKIFGVETSADPTSYEGKAGMVAWHDDIYAVADCLGVCK"
     gene            complement(1576..1974)
                     /locus_tag="VFF70_10550"
     CDS             complement(1576..1974)
                     /locus_tag="VFF70_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45177.1"
                     /translation="MKTWFTSLNGAVTLSALSLLSFIGYVLMEYRYFLEKWIPGTGAA
                     AVETIAVLLIAGFWLRALFVATAGRRGGFITLLALSLFTILIFIYDLQFMLNTPMLWT
                     EQLMLIVMLIAGVVATVALWPQLRRNKATV"
     gene            complement(2026..2283)
                     /locus_tag="VFF70_10555"
     CDS             complement(2026..2283)
                     /locus_tag="VFF70_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZY45178.1"
                     /translation="MKVIAHLGEPFWRAANTHEVSLMLRDDATVSDACDQLARLYPAL
                     DADLNHGETQPAIFVNDDQADRTTPLIEGAQLYLLWPVSGG"
     gene            complement(2280..3602)
                     /locus_tag="VFF70_10560"
     CDS             complement(2280..3602)
                     /locus_tag="VFF70_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HZY45179.1"
                     /translation="MDDPTGLDMIYDWNEKGETFKPKHRILITDETLRDGLQSPSVTH
                     PSIQDKVYLLYLMRCLEIDAVDLGLCGAGERFKKDVVVLAREIVNQKMNMVPQSAARS
                     LESDISPIADASQEAGIAIEAAIFLGSSPIRQYAEGWDIDTLLRLTEASVSFAVKHGL
                     PVMFVTEDTTRAKPDDLKRIYATAIRAGAHRVCAADTVGHATPNGVHNLVRFLRAAID
                     EIDPNVGIDWHGHKDRGLDIANSLAAIEAGADRVHACGLGIGERSGNTPMEILLVNLN
                     LLGLANRDLQGLPEYTQVVSQACGVPIPFNYPIVGADSFRTATGVHAAAIAKAVNRHD
                     NWLSERVYCGVPASMVGREQGIEIGPMSGEHNVRAWLAKHNIEAHPMFIDKILAAAKR
                     SNHLLTEEEIQRMTRVLKLRMGMGQTEVTDSDMDILFPQMAQPAGAAR"
     gene            complement(3773..4399)
                     /locus_tag="VFF70_10565"
     CDS             complement(3773..4399)
                     /locus_tag="VFF70_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZY45180.1"
                     /translation="MNDTTNLIFAGVGGQGVLLIAEITALTAVRAGYDVKQTEVHGVS
                     QRGGSVESHVRFGEVVHSPLVTPGQADVVIGLEKLEGLRYANFVRPSPPAPRPTQGEG
                     GILLVNDHEIIPGSVANAVERYPHNSIDFLRDKGVRVIAVPASRLAQDLGDGRMANIV
                     LLGALSTLIDLPEAAWQETLKAKLPAKYRDANLRAFTVGKESIAVQAA"
     gene            complement(4406..5008)
                     /locus_tag="VFF70_10570"
     CDS             complement(4406..5008)
                     /locus_tag="VFF70_10570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY45181.1"
                     /translation="MKQFYEITYRYFRAPWDIGAREELVSLVDGGCIKPCRAVDLGCG
                     AGANAIYLAQHGFDVTGVDYAEAAIEKARARARDAGVQVNFIVDDLTNPLHVSGTFDF
                     LLDFGVLDDLRPRQREPYLRNMLPLTHSDSHYLLWGFEYPIRRWEKFMPFYDVPFYPG
                     EIEQRFGQYFEIEKTASKLDYSKWPPGYAAYFMTRKGETT"
     gene            complement(5080..6870)
                     /locus_tag="VFF70_10575"
     CDS             complement(5080..6870)
                     /locus_tag="VFF70_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZY45182.1"
                     /translation="MKELLLGNEAIARGAWEAGVKFAAGYPGTPSTEILETIAKKYPE
                     VDAQWSANEKVAFEEGMGAAIGGLRVLVTMKMVGLNVAADSFMVFPYAGTNGGFVVIS
                     ADDPGMHSSQNEQDNRYLALMAKVPVIEPSDAQECKDFIKLGMELSEQFSTPVMLRTT
                     TRLAHHKGLVELGERTEVAQKDFVPDPKRFSVPIYRTLLRPGLEKRLADLREYAETTP
                     LNRREWRTGRSGVEAAKGSIGFVTSGIAYQYVREVMPDASVFRLGMTHPLPARSLREF
                     CAAHETVYVVEEGEAFIEDYIKSIGITNVIGKSIFGVIGEYSPDRVAKAIGIGNSTAL
                     NFDQEISILPRPPMFCVGCGHRTVFDSLRQLKVTVAGDIGCYTMGALPPYEASHTTFC
                     MGAGIGTAFGLERAGHDRTVAIIGDSTFVHSGLPSLIDAVYNGSSLTLIILDNSTTGM
                     TGQQPNSSAGKTLRGKPAPVLNLEALVRAAGVQQVQVVDTWERKEVVRAIRSALAHQG
                     PAVVIAKGPCMRLPEMKLSERDETPFFIDESLCTKCDACFKVWCPAITRTALGFPIID
                     GLECTACTVCAQVCPVDAIMPIDERQPVKV"
     gene            complement(6867..>7670)
                     /locus_tag="VFF70_10580"
     CDS             complement(6867..>7670)
                     /locus_tag="VFF70_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZY45183.1"
                     /translation="EFIESQYDGDIWALAEQVLTPTGLRPFLQSSGIDWAVALAEVNP
                     ATTGNTPNQYVADLCAQANALPDPATGPRGRLLPFASINPYMINDLAAEFEKWVKDFG
                     FRGLKAYPVYQHHYMNDTRMYPLYAKAQELDVPLLVHTGSSVFKGARIKYGDPLHLDD
                     VAIDFPNLTILMAHSGRPFWYQQAFWMARQHKNVFMEISGLPAKKLLEYFPDLERLAD
                     KVVYGSDWPGNPHLRRNVEAILALPLTDETKEKILWRNAATILNVEVRG"
BASE COUNT         1598 a         2271 c         2089 g         1712 t
ORIGIN      
        1 acttgcagac gcccaaacaa tcggcgacgg cgtagatgtc gtcatgccac gcgaccatgc
       61 ccgctttgcc ttcatacgac gtgggatcgg cgctggtttc gacgccgaaa attttcatgc
      121 gcacgtcgtc gggcaggcgc agaatatcga gcgtcgggcg gttgcggaga tgatccgcgc
      181 cgcgtgaagc gaccgcgatg ccaagcgcga atgatttgat gtaacgcaca tcatgcggat
      241 cggattgcgg caggtctttg acggcgatca aataatcggc ggcttccagc gggaaaaact
      301 ttttggcttg cgtgctttcg gccaagatat ttccgaagcc gcgtcgataa gcgatgtcat
      361 tgatcaattc ataagcgcgc tcgtaatcgc cccagtccag cgggccgtcc gtcatatcag
      421 ccgtgatcaa gccgcgttga tacaactcca tcgcccatgc gatgatcgtc ccgaccgatg
      481 acgcatcgag gccgaggtcg ttcaccagat tgttgagcat gatgacttga tcggtcggcg
      541 cgaggccgat gttcgcgccc agcagcccga tcgtggaata ctcgggccct tcgccgccga
      601 gcgtgttgcg atgtcggcag tgcacgacgc acgacgtgca ggccagcatc ttgtctgaaa
      661 atttttcgat ctcggtggca ttcaaggtgt cggcaaagaa attgtcttga ctgttgcgcg
      721 tgcgaatcgc gcccaggcga ttgctgactt catacaacag cggcgtgcca agtttgccaa
      781 gtgtctgaat cactttggag ccttgcaaat attttgtctg ctccaagcgt gtagcgcgca
      841 gcatttcttt atcgtccacc ggaatcgatc cgccgccagt cgcgacgatc gccttgaggt
      901 tcttgctgcc catgaccgcg cccatgccgc agcgcccggc ggtgttcttc aagccggtca
      961 tgatcgacgc catgcgaact aagttctcgc ccgccgggcc gatcaccgcg ctgaccgtgc
     1021 tgccgccgtg tttcgctttc aacatctcct gcgctttggg cgaagtggtg ccccatattt
     1081 catcggcggc gtggatcgag atgcggccag cttctaataa caggtaactc ggccgatcgc
     1141 atttgccgac gatgatcaat cgatcgaaac cggctttgcg catcgccgcc gcaaaaaatc
     1201 cgccgcagtt cgcatcgccc agtcccagtg tctccggcga cttggcgctg acgttgaagc
     1261 gcgcggcatt cggcgcgatc gtgccggtga gtaagcccga tccaaaaatg agcggattat
     1321 cggggccgag tggatcgatc gatttgggat cgcccgccac attcagatac ttgcggagat
     1381 agtgcatgtt caagccgcgt ccgccgaggt aagcacggat caaccgatcg ggcgtccgat
     1441 cgatctcaat gcggctggtg gttaaatcga ttatggctgt gttgggcata ggttcatctt
     1501 caatcttgcg aagggtttag tgtcgatcga ctattcctca agccagcctt cgcaaggttg
     1561 tgctttcaga ttgtgttaga ccgttgcttt gtttcgccta agttgcggcc agagcgcgac
     1621 ggtcgccacc acgccagcga tgagcatcac gatcagcatg agttgctccg tccacagcat
     1681 cggagtgttg agcataaatt gcaaatcgta gatgaagatc aatatagtga aaagactgag
     1741 ggcgagcaac gtgatgaagc cgccccgccg gccggcagtg gcgacaaaaa gcgcacgcag
     1801 ccagaacccg gcgatgagca gaacggcgat tgtttctacc gccgccgcgc cagtgcccgg
     1861 aatccatttt tcaagaaagt agcggtactc catcaaaacg tagccgatga acgacagcag
     1921 tgacagcgct gagagcgtga ccgctccatt gagagacgtg aaccaggttt tcatttgatt
     1981 ttctccctga ttgaattatt ggatgctgag attcttgatc gttgatcagc cgccgctcac
     2041 cggccacaac aaataaagct gtgcgccctc gatcagcggt gtggttcgat cggcttgatc
     2101 gtcattcaca aagatcgcgg gctgcgtttc gccgtgattg agatcggcat cgagcgcggg
     2161 atataagcgc gccaattgat cgcacgcatc agatacggtc gcgtcatcgc gtaacatcaa
     2221 acttacttca tgcgtgttcg ccgcgcgcca aaacggttcg ccgagatgag cgatcacttt
     2281 caccgcgccg ctcctgcggg ctgagccatt tgcggaaaaa ggatgtccat atcgctgtcg
     2341 gtcacttcgg tctggcccat gcccatacgc agtttcagca cacgcgtcat acgctgaatt
     2401 tcttcttcgg tgagaaggtg attcgatcgc ttggccgcgg ccagaatctt gtcgatgaac
     2461 atcgggtgcg cttcgatgtt gtgcttggcc agccacgcgc gcacattgtg ctcgccggac
     2521 atcggaccaa tctcgatgcc ttgttcgcgc ccgaccatgc tggccggcac gccgcagtac
     2581 acgcgctcgg acaaccagtt gtcatggcga ttgaccgctt tggcgatcgc ggccgcatgc
     2641 acgcccgtcg cggtgcggaa tgaatctgcg ccgacgatag gatagttgaa gggaatcggc
     2701 acgccgcacg cttgcgacac gacttgcgtg tattctggca gaccttgcag atcccgattg
     2761 gctaagccga gcaaattgag attgaccaac aggatttcca tcggcgtgtt gcccgatcgt
     2821 tcgccgatgc ccagcccgca cgcatgaact cgatcggcgc cggcttcgat cgcggcgagc
     2881 gagttcgcaa tatcgaggcc gcgatctttg tggccgtgcc aatcgatgcc gacgttcgga
     2941 tcgatttcat cgatcgctgc acgcaagaac cgaactagat tgtgcacacc attcggcgtt
     3001 gcatggccga ccgtgtctgc cgcgcaaacg cgatgcgcgc ccgcgcggat cgccgtggcg
     3061 taaattcgtt tcagatcgtc cggcttggcg cgcgtggtgt cttcggtgac gaacatcacc
     3121 ggcaggccgt gcttcacggc aaacgacacc gacgcttcgg tcaggcgcag cagcgtgtcg
     3181 atgtcccagc cttcggcgta ttgtcgaatg ggactggagc cgagaaagat cgctgcttcg
     3241 atcgcgatgc cggcttcctg ggacgcgtcg gcgatcgggc tgatgtcgga ctccagcgag
     3301 cgcgccgcgc tctgtggcac catgttcatt ttttgattaa cgatctcgcg cgccaagacc
     3361 accacgtctt tcttgaagcg ctcgcccgcg ccgcacaaac ccagatcgac cgcatcgatt
     3421 tccaggcagc gcatcaaata caacagatac actttgtcct gaatcgacgg gtgcgtcacc
     3481 gacggcgatt gcaagccatc gcgcagcgtt tcgtccgtga tcaggatgcg atgcttcggc
     3541 ttgaaggttt cgcctttttc gttccagtcg taaatcatat cgagtccggt tgggtcgtcc
     3601 atgagatgcc tcctgtgatt tcgtgttgcg ctcttcgatc gtcattgcga ggaggccgta
     3661 ggccgacgaa gcaatctcag cagactatcg tggagattgg ttcgcttcgc tcgcaatgac
     3721 ggtatgccca tttatttcac cagtgtaatc cgtgtaatcc gtggtaaatt tattaagccg
     3781 cttgaaccgc gatcgattct ttcccaactg taaacgcgcg caaattcgcg tcccgatatt
     3841 tcgcgggcag ttttgctttc aaggtttcct gccacgccgc ttccggcaga tcgatcaacg
     3901 tagacagcgc acccagcagc acgatgttcg ccatacggcc atcgcccaaa tcctgtgcca
     3961 gccgcgaagc cgggacggcg ataacgcgca cgcctttgtc gcgcagaaaa tcgatcgagt
     4021 tgtgcgggta tctttcgacc gcatttgcca ccgagccggg aatgatctcg tgatcgttca
     4081 cgagcaagat tcccccctcg ccctgagtag ggcgaggggc cgggggtgag ggtcgcacga
     4141 agttcgcata tcgcaaccct tccaacttct caagcccaat caccacatcg gcttgacccg
     4201 gtgtcaccag cggcgagtgc accacttcac caaagcgcac gtgactttcc acgctgccac
     4261 cgcgctgcga cacgccatgc acttcggttt gcttcacatc gtatccggcg cgcacggcgg
     4321 ttaatgcggt gatctccgca atcaacaaca cgccttgtcc accgacgccg gcgaaaatga
     4381 gattcgttgt gtcattcata gctcatcatg ttgtttctcc tttgcgggtc atgaaatagg
     4441 ccgcataacc gggcggccat tttgaataat caagtttgct cgcagtcttt tcgatttcaa
     4501 aatattgacc gaagcgctgt tcgatctctc cgggatagaa gggaacgtcg taaaacggca
     4561 tgaacttttc ccaccgacgt attggatatt caaaacccca gagcaggtaa tgactgtccg
     4621 aatgagttag cggaagcatg ttgcgaagat atggctcgcg ctgacgaggg cgcaaatcgt
     4681 ccagcacgcc aaagtcgagc aggaaatcga acgtgcctga gacatggagc gggttcgtaa
     4741 ggtcgtccac aatgaagttg acctgcacgc ctgcatctct agcgcgtgct cgcgcctttt
     4801 caatcgcggc ttcggcatag tccacaccgg tcacatcaaa tccatgttgt gcgagataga
     4861 tcgcgtttgc ccctgcgccg caaccgaggt caacggcgcg gcacggcttg atgcatccgc
     4921 cgtccaccag cgacacgagt tcctctcgcg ctccgatgtc ccacggtgct cgaaagtatc
     4981 ggtatgtaat ttcataaaat tgtttcatgg ctgtcgatca aaccctgtga tgtcaatgtg
     5041 actgctgctt gtgtcgttgc gcgggctaac tattgccact cacactttca ctggctgccg
     5101 ttcatcgatc ggcatgatcg catcgacggg acacacttgt gcgcagaccg tgcaagccgt
     5161 gcattccagt ccatcaatga tcgggaaacc cagcgccgtg cgcgtgattg ccggacacca
     5221 cactttgaag catgcatcac atttggtgca gagcgactcg tcgatgaaga acggcgtttc
     5281 atcgcgctcc gataatttca tttctggcaa gcgcatgcac ggccctttgg caatcaccac
     5341 cgccgggcct tgatgcgcca gcgccgatcg gatcgcgcgc accacttctt tgcgctccca
     5401 cgtgtcgacg acctgcactt gctgcacacc cgccgcgcgc accaacgcct ccagattcaa
     5461 gacgggcgcg ggcttgccac gcaatgtttt tccggcagaa gaatttggct gctggcccgt
     5521 catgcctgtg gtcgaattgt cgagaatgat caacgtgagt gaactgccgt tgtagactgc
     5581 atcgatcaat gacggcaaac ccgaatgcac gaatgtcgaa tcgccgatga tcgccaccgt
     5641 ccgatcgtgc ccggcgcgtt ccaggccgaa tgccgtgccg atgcccgcgc ccatgcagaa
     5701 cgtggtgtgc gaagcttcgt acggcggcag cgcgcccatc gtgtaacagc cgatgtcgcc
     5761 cgcgacggtc actttcaact gccgcaatga atcgaacacc gtgcgatgcc cgcagccgac
     5821 gcagaacatc ggcggacgcg gcaggatgct gatctcttga tcgaaattga gcgcggtgga
     5881 gttgccaatc ccgatcgctt tagcgacgcg atcgggtgag tactcgccga tgacaccgaa
     5941 gatcgatttg ccgatgacgt tcgtgatccc gatcgacttg atgtaatctt cgatgaaggc
     6001 ttcgccctct tcaaccacat agaccgtttc atgcgcggca cagaattcgc gcagcgatcg
     6061 cgcgggcagc ggatgcgtca tgcccaagcg aaagaccgag gcgtccggca ttacctcgcg
     6121 cacatactga tacgcaatcc cgctggtgac gaaaccaatt gagcctttgg ctgcttccac
     6181 acccgatcgg ccggtgcgcc attccctgcg attgagtggc gttgtttccg catattcgcg
     6241 cagatcggcc aatcgttttt ccagaccggg ccgtagcagc gttcgataaa tcggcacgga
     6301 aaagcgtttc gggtctggca caaaatcttt ctgcgccact tcagtgcgct cgcctaattc
     6361 gaccaagcct ttgtgatgtg ccaaccgcgt tgtcgtccgc agcatcactg gcgtactgaa
     6421 ttgttctgaa agttccatcc ccaacttgat aaagtctttg cactcctgcg cgtcacttgg
     6481 ttcgatcacg ggcactttcg ccatcaacgc caagtagcga ttgtcctgct cgttttgcga
     6541 tgagtgcatc ccgggatcat cggccgagat cacgacgaag ccgccattgg tgcccgcata
     6601 cggaaagacc atgaaagaat ccgccgccac attcaggccg accatcttca tcgtaaccaa
     6661 tacgcgcagt ccgccgatgg ccgcgcccat gccttcttcg aacgcgactt tctcattcgc
     6721 cgaccactgc gcatcgactt cgggatattt cttcgcgatc gtttcgagaa tttcggtgct
     6781 gggcgtgccg ggataaccgg cggcaaactt cacgcctgcc tcccacgctc cgcgcgcgat
     6841 cgcttcattg cctaacaata gttctttcat ccccgcacct ccacatttag aatcgtcgcg
     6901 gcgttgcgcc acaatatttt ttctttcgtc tcgtcagtca acggcagtgc cagaattgcc
     6961 tcgacattgc gccgcagatg aggattgccg ggccagtctg atccatacac aactttatcg
     7021 gccagtcgtt cgagatcagg aaagtattcc aacagttttt tcgcgggcaa gccggagatt
     7081 tccataaaca cattcttgtg ctggcgtgcc atccagaaag cctgctgata ccaaaacggg
     7141 cgacccgaat gtgccatcaa gatggtgaga ttcggaaaat cgatcgccac atcgtcaaga
     7201 tgcagcggat cgccgtactt gatgcgcgcg cccttgaaca ccgacgatcc ggtgtgcacc
     7261 aacaacggca cgtcgagttc ctgcgctttg gcgtacagcg gatacatgcg cgtgtcgttc
     7321 atgtaatgat gttgatagac cggatacgct ttcaagccgc gaaagccaaa gtcctttacc
     7381 catttttcaa actcggccgc aagatcgttg atcatatacg gattgatact cgcaaacggc
     7441 agcaatcgac cacgcggacc ggtggctgga tcgggtaacg cattcgcctg cgcacacaaa
     7501 tcagccacat attgattcgg cgtgttaccc gtcgttgccg gattcacttc agccagcgcc
     7561 accgcccaat cgatcccgct cgattgcagg aacggccgca gcccagtcgg cgtcaagact
     7621 tgttcggcca gcgcccagat gtcgccgtca tattgactct cgatgaattc
//
LOCUS       DASRTJ010000523         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_260793,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000523 DASRTJ010000000
VERSION     DASRTJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260793"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFF70_10585"
     CDS             complement(<1..753)
                     /locus_tag="VFF70_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZY45184.1"
                     /translation="MATLRTTYAPADYGRAGWRHFDTPLLVVVLSLIVIGVVMINSAT
                     LNSVDPDIVGVAKRQAIYAVIGIIILLITASIDYRIWQNARKLLYGLTLLFLAITLLA
                     GDSAIGGVRRWIDLSAFPVQPAELAKVLMILALAGFLAARADSIGKMKTVLSSLIYVG
                     IPAGLIFIQPNLSTAIVYLVIWFTMIIAAGARIKHLSFVGLLAALGLPVIWLVMAPYQ
                     RDRILGFLDPSRNPGANYNLTQAQIAAGSGGFA"
     gene            complement(822..1088)
                     /gene="minE"
                     /locus_tag="VFF70_10590"
     CDS             complement(822..1088)
                     /gene="minE"
                     /locus_tag="VFF70_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZY45185.1"
                     /translation="MNWLKRLRGEPQPSSRDTAKQRLQLVLVHDRSQISPGIIEIIKD
                     EIIAVISKHIAIDTAGVQVSFTHGERENKLVADIPLVDGRRTRK"
     gene            complement(1085..1894)
                     /gene="minD"
                     /locus_tag="VFF70_10595"
     CDS             complement(1085..1894)
                     /gene="minD"
                     /locus_tag="VFF70_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZY45186.1"
                     /translation="MTARVITITSGKGGVGKTTTTANLAVALALLGKRVVCMDADIGL
                     RNLDVVLGLENRIVYDVVDVIEGRCKLRQAMIKDKRLSELYLLPASQKRDKSAVKPND
                     LVKVCLDLRPDHDFVLIDSAAGIEEGFRTAIAPADEVIIVTNPEVSAVRDADRIVGLV
                     EAAEKGPAKLIVNRLKPELVQRGDALDTSDVLDILAIELIGIIPEDESVLISTNRGSP
                     VALDDKSRAGKSFRNIARRLTGEDVPFESFRDEAGVFGRFAKFLRGDGRTS"
     gene            complement(1974..>2539)
                     /gene="minC"
                     /locus_tag="VFF70_10600"
     CDS             complement(1974..>2539)
                     /gene="minC"
                     /locus_tag="VFF70_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZY45187.1"
                     /translation="GARVGLIVGPRRLTELDVRAARDLLTRFDVTLWAVVSDDKETRQ
                     VASILGLDADIPKRKAKAGIAEPPIELASSIEEPDLGLVVHRTLRSGQSLRHPGSIAV
                     IGDVNPGAEILAGGDIVVWGRLRGVVHAGAMGDATAVVCALDLVPTQLRIADYISRSP
                     EDKRKRAIPEVARVREGQIVAEAWEAK"
BASE COUNT          590 a          729 c          672 g          548 t
ORIGIN      
        1 ggcgaaaccg ccgctgccgg ccgcaatttg cgcctgcgtc agattgtaat tcgcgccggg
       61 gttgcggctg ggatcgagaa aacccagaat tcgatcacgt tgatacggag ccatgaccag
      121 ccaaataaca ggcaagccga gcgctgccag caggccaaca aaagatagat gtttaatccg
      181 tgcgcccgcg gcgataatca tcgtgaacca aatgaccaaa tacacgatgg ctgtactcag
      241 gttgggttga atgaaaatca agcctgccgg tatgcccacg tagatcaatg aggacaacac
      301 cgttttcatt ttgccaatcg agtcagcccg ggctgccagg aagccggcta aagccagaat
      361 catcaggact ttggctagtt cggcaggctg caccggaaac gccgacagat cgatccagcg
      421 ccgcacaccg ccgatggcgc tgtcaccggc caataaggtg atagctaaga agagcagcgt
      481 caagccatat agaagcttgc gggcgttttg ccaaatgcga taatcaatgc tggcggtgat
      541 cagcaggatg atgatgccga tgaccgcgta gatcgcctgg cgtttggcca cgcccacaat
      601 gtcgggatcg accgagttga gggtggcgct gttgatcatg acgacgccga taacgatcaa
      661 actcaagacc acgactaata agggcgtatc gaagtgacgc caaccggcgc ggccataatc
      721 tgcgggagcg taagttgtgc ggagagttgc cataggtgaa tttctgatct ttaatcaatc
      781 actgatctcg gaaatgacgt tcagagtaca gatgcccaag attacttgcg ggtgcgccgt
      841 ccatcgacca acggaatatc ggcaactaat ttgttttcac gttcgccgtg agtgaaagac
      901 acttgtacgc cggcggtatc gatcgcgatg tgctttgaga tcacggcgat gatctcgtct
      961 ttgatgatct cgatgatgcc gggcgaaatc tggctgcgat catggaccag caccagctgc
     1021 aggcgctgct tggcagtgtc ccggctggac ggttgtggtt cgccgcgcag gcgtttgagc
     1081 caattcatga cgtcctccca tctccgcgca gaaacttggc gaagcgtcca aagacaccgg
     1141 cctcgtcgcg gaacgactcg aagggcacat cttcgccggt taagcgccgc gcgatatttc
     1201 gaaaggactt gcctgcgcgc gatttgtcgt ctaaggcgac tggactgccc cgattggtgg
     1261 agattaaaac tgattcgtct tccggtataa tgccaatcaa ttcgatcgcc aagatatcca
     1321 gcacatcgct ggtatcgagc gcgtcgccac gctgcacgag ttcaggcttc agccgattga
     1381 caatcaattt cgccggtccc ttttcagcgg cttcgaccaa cccgacgatt cgatcagcat
     1441 cccgcacggc cgagacttcc ggattggtga cgataatgac ttcatcggcg ggggcgatgg
     1501 cggtgcgaaa accttcttca atcccggccg cggaatcgat cagtacgaag tcatgatcag
     1561 gccgcagatc gaggcagacc ttcaccaaat cgttcggctt gaccgcactc ttgtcgcgtt
     1621 tttgggaagc gggcagcaga tacaattccg acagacgctt gtctttgatc atcgcttgcc
     1681 gtagtttgca gcggccttcg attacatcga cgacgtcata cacgatccgg ttttccaaac
     1741 ccaacaccac atccaggtta cgcaggccga tgtcagcatc catgcagacc acccgcttgc
     1801 ctaacaaagc cagcgccacg gctagattgg cggtagtcgt agttttaccc acgccgcctt
     1861 tgccagaagt gattgtaatc acgcgtgcag tcatgcgcct ccttaacaat aaagaatgaa
     1921 cacagagtac tgcgcttgcg tttattaatt attcgttggt aattgaaaat ggttcacttt
     1981 gcttcccacg cttctgccac gatctgtcct tcgcgcactc ttgccacttc cggaatcgcc
     2041 cgcttgcgct tgtcttccgg cgagcgcgaa atgtaatcag caatccgcag ttgggtcggc
     2101 actagatcga gcgcgcacac gacagcggta gcatcgccca tcgcaccggc atgcaccaca
     2161 ccgcgcaggc gaccccatac cacaatatcg cctcccgcga gaatttccgc accgggattg
     2221 acatcgccaa tcacggcgat cgatcccgga tgccgcagtg attggcccga tcttaacgtt
     2281 cgatgtacca ctaagcctaa atccggctcc tcgatcgagc tagctagctc gatcggtggt
     2341 tcagcgatac ccgcttttgc tttgcgcttc ggtatatccg catccagtcc taaaatcgat
     2401 gcaacttggc gtgtctcttt gtcatcactg acgacagccc agagcgtcac atcgaagcgc
     2461 gtcagtaaat cacgcgcggc gcgaacgtct aattcggtca agcggcgtgg acctacgatc
     2521 aatccgacac gcgcaccct
//
LOCUS       DASRTJ010000524         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_279323,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000524 DASRTJ010000000
VERSION     DASRTJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279323"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="pstA"
                     /locus_tag="VFF70_10605"
     CDS             complement(<1..607)
                     /gene="pstA"
                     /locus_tag="VFF70_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZY45188.1"
                     /translation="MSRFLTQRLGFAFLTIVTILVVLPIFFVVGYIILKGGSAINWEF
                     LTTAPRDGMRDGGIWPAIVGTFWLTIGTAIFSVPLGVSIAIYLSEYARDNTFTRLIRL
                     AIINLAGIPSVVYGLFGLGLFVLALQFGTSILAGSLTLAIMTLPVIISTSEEALRSVP
                     QSFRTVSVSLGGTKWQTIRKIILPQALPGILTGVILGLERAA"
     gene            complement(840..1739)
                     /gene="pstC"
                     /locus_tag="VFF70_10610"
     CDS             complement(840..1739)
                     /gene="pstC"
                     /locus_tag="VFF70_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZY45189.1"
                     /translation="MNTKRSSMIEFFAERAIRISGLVAIAFVVLIFLFLLEQGLPAFG
                     ETPIDNLLSTRWYPIEDHFGLLPLILGSLIVTIGAAIIAIPTGVLAAVFVAEVAPRWL
                     REILKPMIEVVAGIPSVVLGFIGIVVLSPVVRTTFNAPTGLTAFTGSLLLAYMALPTI
                     ISVAEDALDAVPRSYRDAALALGATKWQTIWMVTIKAARSGIVTAIMLGIGRAIGETM
                     AVMMVTGNAARMPLSLDSFFLPVRTMTATIAAEMGEVAKGSTHFHVLFAIGIVLFVIT
                     FVVNLLASVAIFRQTRRSTRLLS"
     gene            complement(1813..2685)
                     /locus_tag="VFF70_10615"
     CDS             complement(1813..2685)
                     /locus_tag="VFF70_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY45190.1"
                     /translation="MRSGLLRLALVVFLLAGCARSGSSSAPGGAKPIENIGSDTIVNL
                     ALAWAEAYQKINPNVRISVTGGGSGTGLAALINGTADIANASRAIKPEEIQQAQQNKI
                     NPFEIQIARDAIAVIVNPANPVSQLTLQQISDIYSGKITNWKEVGGEDRPIVLVSRET
                     NSGTHVYFLANVIRMGNSKDTTLFSPDTLLLPSSEGIMDEIKQNPNAIGYDGLGYISS
                     AVKVIAVADQPGDPYILPSIDSVNAGTYAVARPLFMYTNGEPQDLIKAYLDWILGPDG
                     QKIVAELGFVPLMK"
     gene            2809..3558
                     /gene="pstB"
                     /locus_tag="VFF70_10620"
     CDS             2809..3558
                     /gene="pstB"
                     /locus_tag="VFF70_10620"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZY45191.1"
                     /translation="MDKIEVEHLNFYYSNTQALMNISLKVPDRHITALIGPSGCGKST
                     FLRCLNRMNDTIADTRFEGKIILDGEDILTPITNVVDLRRRVGMVFQKPNPFPQSIYD
                     NVAFGPRALKMNSLSLDQIVEQSLRGAALWDNVKDHLKDSALELSLGQQQRLCIARVL
                     AVQPEVILMDEPCSALDPIATLKIEELMQQLKEKYTIVIVTHNMQQAARASDFTGFFW
                     LGKLVEHNTTNQMFVRPKEKQTEDYITGRFG"
     gene            3558..>3841
                     /locus_tag="VFF70_10625"
     CDS             3558..>3841
                     /locus_tag="VFF70_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45192.1"
                     /translation="MTTGGKEGRWNMMRSRLSFAKYPWFQNGIPGAVANLIISGSCLS
                     CAIWFAASASLSSQTVSTIPELILVYAVPIFLIGFAAGVISYRHSFGMVMT"
BASE COUNT          951 a         1056 c         1007 g          827 t
ORIGIN      
        1 ccgccgcgcg ttcgaggcct aagatcacgc ctgttaatat tccgggcagc gcttggggca
       61 gaatgatctt gcgaatcgtc tgccactttg tgccgccgag gctgacactc accgtgcgaa
      121 aactctgcgg caccgatcgc agcgcttctt ccgaggtgct gatgatcacc ggcaacgtca
      181 tgatcgccaa agtcaatgaa cccgccaaga tcgatgtgcc gaattgcaag gccagtacaa
      241 acaagccgag tccgaacaac ccgtacacca ctgatggaat tcccgcgagg ttgataatcg
      301 ccaatcggat taaacgcgta aacgtgttat cgcgcgcgta ctcgctcaag tagatcgcga
      361 tcgatacgcc gagcgggaca gagaagatcg ccgtgccgat cgtcaaccag aacgtgccga
      421 cgatggccgg ccagatgccg ccgtcacgca tgccgtcgcg cggtgcggtc gtcagaaatt
      481 cccaattgat cgcggagccg cctttgagaa tgatgtaacc gacgacaaag aagatcggca
      541 gcacgaccaa gatcgtgaca atagtgagga aggcgaaacc tagacgttga gtgagaaatc
      601 gagacatgcg aaactaacca cggatgacta ggatgtcact gatattttct ctgacaatat
      661 gtgctgttgt atttcaaatg aactgtcgca aagtagaaaa tcagatcaac gcgtagttta
      721 gcacttcacc tcatacgatg tcatttcgaa ccgcgcagcg gtgagaaatc tcagccaccg
      781 attaacaatt tagcaggcag agatagagat tcctcgcttc gctcggaatg acagttctct
      841 cacgacagca atctcgtcga tcgacgggtc tggcggaaga tggcgacgga cgccaataaa
      901 ttgacaacga aagtaatcac aaacaacaca atcccgatcg caaacaatac atgaaagtgc
      961 gtgctgcctt tagccacttc gcccatctcg gcggcgatcg tcgcggtcat cgtgcgcacc
     1021 ggcaaaaaga acgaatcgag cgagagcggc atgcgcgcgg cattgccggt gaccatcatc
     1081 accgccatcg tctcaccgat cgcgcggcca atgcccagca tgatcgccgt cacaataccg
     1141 gatcgagcgg ctttaatggt caccatccaa atggtctgcc acttcgttgc gcccaaagct
     1201 aaagccgcat cccgatacga tcgtggtact gcatcgagcg cgtcttcagc cacgctgatg
     1261 atcgtcggca gagccatgta agccagtagt agcgatccag tgaacgcggt caaccccgtc
     1321 ggggcattga aggttgttcg taccactggc gagagcacca caatcccgat aaagcccagc
     1381 accaccgatg gtatgccagc cactacttcg atcatcggtt ttaatatttc acgcagccaa
     1441 cgcggcgcga cttcggccac aaagaccgcg gccagaactc cggtcggtat tgcaatgatc
     1501 gccgcaccga tcgtgacgat caacgatcca aggatcaacg gcagtaaacc gaagtgatcc
     1561 tcgatgggat accagcgcgt cgagagcaga ttatcgatcg gggtttcgcc gaaagccggt
     1621 aagccttgct caagcaaaaa gaggaagatc aacacgacaa acgcaatcgc tacgagaccg
     1681 ctgatccgga tcgcacgttc ggcaaagaat tcgatcatgg atgagcgttt ggtattcata
     1741 tcgaatcaaa tttgctcctc gcggcgtccc cgccgcgaga tcggtcggcg agacgccgac
     1801 cggagcagca tactatttca ttaacggcac aaaacccaat tcggccacga tcttttgtcc
     1861 gtcggggccg agaatccaat cgagataagc tttgatcaaa tcttgtggtt cgccgttggt
     1921 gtacatgaag agcggtcggg ccacagcata cgtgccggca ttgactgaat cgatgctggg
     1981 cagaatatac ggatcgcccg gctggtctgc cacggcgatc accttcaccg ccgatgagat
     2041 gtagcccagc ccatcgtagc cgatcgcgtt aggattctgc ttgatctcat ccatgatgcc
     2101 ttcgctcgat ggcagcaaca gcgtgtcggg cgagaacaga gttgtgtctt ttgaatttcc
     2161 catgcggatg acattcgcta aaaaataaac gtgcgtgccg gaattcgttt cgcgcgagac
     2221 tagcacaatc ggccgatctt cgccgccgac ttctttccaa tttgtgatct tgccgctgta
     2281 aatatcggag atttgttgca acgtaagttg gctgacagga ttggccggat taacgatgac
     2341 cgcaatcgca tcgcgcgcga tttgaatctc gaatggattg atcttattct gttgggcctg
     2401 ctgaatttct tcgggcttga tcgcgcggct ggcgttggcg atatcggccg tgccgttgat
     2461 caaagcggct aagccggtgc ctgatccgcc gccagtgacg gagatgcgca cattcggatt
     2521 gatcttttga taggcttctg cccaggccag tgcgaggttc acgatcgtgt cggagccgat
     2581 attctcgatc ggcttagcac cgcccggcgc tgatgacgaa cccgatcgcg cacagccggc
     2641 gaggaggaac actaccaacg cgagtcgtag cagacctgaa cgcatcgacg cgattatatc
     2701 atgagatcat cgccgcgctt gtgagttcta tctcttcaag ttacaatcgt gtccgcagat
     2761 tcgcactgat aaattaatct gtgtaatctg cgcgcatctg aggacaccat ggacaagatc
     2821 gaagtcgagc atctcaactt ctattacagc aatacgcaag cgctgatgaa tatctcgctc
     2881 aaggtgcccg atcggcatat caccgcgctg atcggtccca gcggctgcgg caaatcgacg
     2941 tttctgcgct gcctcaatcg catgaacgac accatcgccg atacgcgctt cgagggcaag
     3001 atcattcttg acggcgaaga catattaact cccatcacca acgtcgtcga tctgcgccgc
     3061 cgcgtcggca tggtctttca gaaaccaaac cccttccccc aatcgatcta cgacaatgtc
     3121 gccttcgggc cgcgcgcact caaaatgaat tcgctgagcc tcgatcagat cgtcgaacag
     3181 tcattgcgcg gcgcggcgtt gtgggacaac gtgaaagatc accttaaaga ttccgcgctc
     3241 gaattgtctc ttggccagca gcaacgactg tgcatcgcgc gcgtcctcgc cgtgcagcct
     3301 gaggtgatct tgatggacga gccgtgttcc gcgctcgatc cgatcgccac gctcaagatc
     3361 gaagagttga tgcagcaatt gaaagagaag tacacaatcg tgatcgtcac gcacaacatg
     3421 cagcaagccg cgcgcgccag cgacttcacc ggcttcttct ggctcggcaa gttggtcgag
     3481 cacaatacca ccaatcaaat gtttgtccgt ccgaaagaga agcagaccga ggattacatt
     3541 acggggagat ttggttaatg accacgggtg gtaaagaagg acgatggaat atgatgcgta
     3601 gtcgtctcag cttcgcgaaa tatccatggt ttcaaaatgg aattcctgga gcagtagcaa
     3661 acttgatcat cagtggaagt tgtctatcat gcgccatctg gtttgcagct tcggcatctc
     3721 taagtagtca aactgtttcc accataccag agctaatctt ggtctatgcg gtgccgattt
     3781 ttctaatagg gtttgcggca ggtgtaatat cctaccgaca tagttttggg atggtaatga
     3841 c
//
LOCUS       DASRTJ010000525         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_203354,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000525 DASRTJ010000000
VERSION     DASRTJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203354"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1601
                     /locus_tag="VFF70_10630"
     CDS             312..1601
                     /locus_tag="VFF70_10630"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZY45193.1"
                     /translation="MPSNTLSITDNRTGKTYEVPIENETIKTADLRQIKVNATDFGLM
                     GYDPAFMNTASCKSTITYIDGDLGILRYRGYPIEQLAEKATYLETAYLVLYGELPNQQ
                     QLADWSYNVTHHTILHEQMKKLMDAFHYDAHPMGMLISTIAALSTFYPEAKLIRDAEA
                     RQKQIWRLIGKLPTIAAFSYRHNRGMPYAYPDNDLSYTGNFLNMMFKTTEIKYQPNPI
                     LEHALDVLFILHADHEQNCGTNAMRAIGSSNVDPYSAMAGAAAALYGPLHGGANEEVL
                     LMLNKIGSKANVPEFIKHVKAGEGKLMGFGHRVYKNYDPRAKIIKKVADEVFAVTGRN
                     LLLDIALELERIALEDDYFVTRKLYPNVDFYSGIIYQAMGLPVEMFPVLFAIPRTSGW
                     LAQWKEMLDDADQKIARPRQIYLGADERNFTQMDKRK"
     gene            1733..2626
                     /locus_tag="VFF70_10635"
     CDS             1733..2626
                     /locus_tag="VFF70_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HZY45194.1"
                     /translation="MKHALSISLGSTARDKVVEVKLLGETVRIERRGTNGDIQKATEL
                     FNELDGKVDAFGVGGIDLGEHTPWKFYPLHDAQRMVKNVKHTPMVDGGGLKTTLESRV
                     MQFIEKQLIIDPKRAFLVAAITRYGMTESFIAAKYDCVFGDMMFALGMPIAIRSTTTL
                     RRLAKILLPIVGRMPIEWLYPTGEKQDRNTPKYEKYYQWASVTAGDFLYVKQHLPLRM
                     DGKIILTNTTTVEDVEWLKQRGVKALVTTTPVYDGRSFGTNMMEAALVAAAGKGRVLA
                     HEELIEIIDQLKLEPQIQKLN"
     gene            2728..3513
                     /locus_tag="VFF70_10640"
     CDS             2728..3513
                     /locus_tag="VFF70_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZY45195.1"
                     /translation="MDVVISAGSTPGEIDPLLKYTKVDRKALIPIAGNPMIKWVTDAF
                     VASSHIDRLIIIGLDQSIGIDFGSKPVIYVPSTGKLVQNYIAGVERALQDTTDSKRIL
                     IAACDIPLITTAIVDDHIDTMLATDHEVYYSIVRKATMLAKFPTSKRSYTCLRDGVFC
                     GGNINMISGRVMASESRKLWDKLLERRKNVFQQAALAGYGTLFKLLIGRLTIAAAEQR
                     AEKIMGVKCKAIETPHAEIGMDVDKPFQLDIATAALNIKLGLS"
BASE COUNT          914 a          924 c          906 g          771 t
ORIGIN      
        1 acgcgcgcgt gcctagtcgc cgatggcata ggaccgccat cgctataaaa tcataaaccg
       61 cccttccggc atgcttcgcg cactgccgga atcttaaacg gagccgatgc aagccatcga
      121 ctccgttttc gtttcaaatg cggtaaaata tcaacaaccg ctcacgtgaa aaacaatttg
      181 tcatgtgcag ggcatgtcat gtgtcaattg acacccgccc tcaaccttac taaaattatt
      241 caagtcggga taaactttaa tctgcaaatt tgtttttagt caaacctaat ccagtttaga
      301 ggagaacact catgcccagc aacaccctaa gcatcaccga taatcgtact ggcaaaacgt
      361 acgaagtgcc gatcgagaac gaaacgatca aaaccgccga tctgcgtcag atcaaagtca
      421 acgccaccga cttcggcttg atgggttatg atccggcctt catgaatacc gcgtcgtgca
      481 aaagcacgat cacctacatc gacggcgatc taggcatctt gcggtatcgc ggctacccaa
      541 tcgagcagct ggccgaaaaa gccacttacc tggaaacggc ctatctggtc ttgtatggcg
      601 aactgccgaa ccaacagcag ttggctgatt ggagctataa tgtcacgcat cacaccattc
      661 tgcacgagca gatgaagaag ttgatggatg ccttccatta tgacgcgcat cccatgggca
      721 tgttgatcag caccatcgcc gcactgtcca ccttttatcc tgaagccaaa ctcatccgtg
      781 atgccgaagc gcgccaaaaa caaatctggc gcttgatcgg caagctaccc accatcgctg
      841 ccttctcgta tcgtcacaat cgaggcatgc cctatgctta ccccgataac gatctgagtt
      901 acactggcaa tttcttgaat atgatgttca aaacgaccga gatcaaatat caaccgaatc
      961 cgatcctgga acacgcgctg gatgtgctct ttatcctgca cgccgatcac gagcagaatt
     1021 gcggcaccaa tgccatgcgg gccatcggca gttccaacgt cgatccgtac agcgcgatgg
     1081 ccggtgccgc cgctgccttg tatggcccgc tgcacggcgg cgcgaacgaa gaagtgctgc
     1141 tcatgctgaa caagatcggc agtaaggcca acgtgccgga atttattaag catgttaaag
     1201 ccggcgaagg aaaattgatg ggttttggac atcgcgtcta caagaattac gatccccgcg
     1261 ccaagatcat caagaaggtc gcggatgaag tctttgcggt taccggccgc aatctgctgc
     1321 tcgatatcgc gctcgaactg gaacgcatcg ccttggaaga tgattacttc gtgacacgca
     1381 aactgtatcc gaatgtcgac ttctattccg gcatcattta tcaagccatg ggcttgccgg
     1441 tcgaaatgtt cccggtgctg ttcgccattc cgcgcacgtc cggctggctg gctcagtgga
     1501 aagaaatgct ggatgatgcc gatcaaaaaa tagcacggcc gcgccagatc tacctcggtg
     1561 ccgatgagcg caacttcacg cagatggata aacgcaagta gactctcagc ctcctgaagg
     1621 ttctaaacct tcggcaggtt ttttcattgg cacattcacg ccgatcgaca tacaatatcc
     1681 gccagtgcat cgatgatgtg ctggcggttt ttttgttgtt catgggggag acatgaagca
     1741 tgcactcagc atcagcttag gcagcaccgc gcgcgataaa gttgtcgaag tgaaattgct
     1801 cggcgaaacc gttcgcatcg agcggcgcgg cactaatggc gacatccaga aagcgaccga
     1861 acttttcaac gaactagatg gcaaggtcga tgcgttcggc gtcggcggga tcgatctggg
     1921 cgagcatacg ccatggaagt tttatccgct gcatgatgcc cagcgcatgg ttaagaacgt
     1981 taagcacacg cccatggtcg atggcggcgg cttgaagacc acgctcgaaa gccgcgtgat
     2041 gcaatttatt gaaaagcaac taattatcga tccgaaacgc gccttcttgg tcgccgcgat
     2101 cacgcgctat ggcatgaccg aatctttcat cgctgcaaaa tatgactgcg tcttcggcga
     2161 catgatgttc gcgctcggga tgccgatcgc catccgcagc acaacgacgc ttcgacgttt
     2221 ggcgaaaatt ctgctcccga tcgtgggccg tatgccgatc gagtggttgt atcccacggg
     2281 tgagaagcag gatcgcaata caccgaagta cgaaaaatat tatcaatggg ccagcgtgac
     2341 tgcgggcgat ttcctgtacg tcaaacagca tctgcccttg cgcatggacg gcaagatcat
     2401 cctcaccaac accaccaccg tcgaagatgt cgaatggctg aaacagcgcg gtgtcaaagc
     2461 actggtgact actacacccg tctatgacgg tcgatcgttc ggcacgaaca tgatggaagc
     2521 cgcactcgtc gccgctgctg gcaaagggcg agtgttggcg catgaagaac tcattgagat
     2581 catcgatcaa ttgaagttag aaccacagat tcaaaagttg aattgaaccc cctcccggcc
     2641 tcccccgttc ggaaaagatc gaacagggga ggagttgttc cttcccatgc gaagcggggg
     2701 aaggctagaa ggggcaaagg gaacatcatg gacgtggtca tcagcgccgg atcgacgccg
     2761 ggcgagatcg atccgctgtt gaaatacact aaagtcgacc gcaaagcgtt gatcccgatc
     2821 gcgggcaatc cgatgatcaa atgggtgacc gatgcgtttg ttgcatcaag tcacatcgat
     2881 cgattgatca tcattgggct ggatcaatcg atcgggatcg acttcggctc aaagccggtt
     2941 atctatgttc catcaacagg caagttggtg cagaattata tcgccggagt ggagcgggct
     3001 ttgcaagata caaccgattc aaaaagaatc ttgatcgccg cgtgcgatat tccgttgatc
     3061 accacggcga tcgtggacga tcacatcgac acgatgctgg ccaccgatca cgaggtgtat
     3121 tacagtatcg ttcgcaaagc gacgatgctg gcaaagtttc caacatcaaa gcgatcctac
     3181 acgtgcctgc gcgatggggt tttctgtggc ggcaatatca atatgattag cggccgagtg
     3241 atggcaagtg aatcacgtaa attgtgggat aaattactcg aacgaagaaa aaatgttttc
     3301 caacaagcag cgttagcagg ttatggcact ttgttcaagt tactgatcgg ccgattaacg
     3361 attgccgccg ccgagcagcg cgccgaaaaa attatgggcg tgaagtgcaa ggcgatcgag
     3421 acgccgcacg ccgagattgg tatggatgtg gacaagccat ttcagttgga tattgcaaca
     3481 gcggccttga atataaaatt aggactgtca tgaca
//
LOCUS       DASRTJ010000526        10250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_260977,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000526 DASRTJ010000000
VERSION     DASRTJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260977"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFF70_10645"
     CDS             <1..207
                     /locus_tag="VFF70_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011273238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZY45196.1"
                     /translation="DTGIKLGLNHPMGPFTLMDFIGLDTCLFIGDAMYDEFKDARYAA
                     PPLLRRMVTAGLMGRKSKQGFYQY"
     gene            285..2114
                     /locus_tag="VFF70_10650"
     CDS             285..2114
                     /locus_tag="VFF70_10650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZY45197.1"
                     /translation="MFRSSAQRLIAILIALLAVPTLIAAAQSSEWLTYSDPVAGFAFE
                     YPSNAHLSTGLDASQGNSSVFVSIGAGQEYQGYSIAVLNNGRNLALDQFLIELLHLDT
                     AQSKRLNLNGLEAIQQPATGADQAEAYWVQLDRVVLKIELYRGDGSIEITPDSRAAFD
                     RALATLRSIPQSQTIAPSSIAPNVPNIGIADQFQAPLATATTIQYETQWNVITSDTRY
                     GVRNLALTGRKCFGVAWNRMLHSAIDLYRLDGQSADGTSVVAVADGQVAYYDSSYASY
                     PGHVVIIRHQLADSSLIYSVYAHMNAVYIYQGQNVIRGQPIGTVLYQPNDSHLHFEMR
                     KFLDGTYIYTGYPACNGIIYGRGYTYQIHPDNFPAPNGYINPDAFIQAHGGSPLTPIG
                     LPDAYGPLLTYSTLNASTSIDQAQPITDSPSMTHTAYFPLIVREYPKREPACVEGQNL
                     LTNSGFEDGVGSAPWVQVRNSTADSIDANWPYSGVYSVWLGGRNLADEEALQSFVVPY
                     YTDGITLTFKRYLTTQETALNTVYDHFEVVLENTVGNEISPQLQFSNLSTKNVWMAES
                     AAFDGAQAWTGQRLRLSIKGMTDGSLPSSFFVDEVSVQTRCAP"
     gene            2304..3917
                     /gene="ppx"
                     /locus_tag="VFF70_10655"
     CDS             2304..3917
                     /gene="ppx"
                     /locus_tag="VFF70_10655"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZY45198.1"
                     /translation="MQHKIGIIDLGSNTSRLIIMAYTPGVTFQLIDQLRERVRLSEGM
                     GAENILRPEPMERTIRLLKVFRQLCEANGIDTIVATATSAARDARNQAEFLERVRTEA
                     GLDLRILSGAEEAYYGYLGAVNSLPIQNGMVVDIGGGSLELARVQDRQLAQTASLPLG
                     AVRLTETFIKNDPPKSLDLRVIDRYIDAMLGSIDWLKAGSGGMLVGLGGTARTLAKID
                     QERRHYPLDRLHGYTISLTSIENILHELEQLPLSKREKVPGLSADRADVIISGAIALT
                     RLMHQGGYHEMTICGQGLREGLFYEQFLKDSGTALVPDVRAFGLANLTYLYNINWPHA
                     RHVQQLALSMFDQLRSLHGCGPLERAILSGAALLHDVGVAIDFYDHDQHSYYLILNAE
                     LPGFSHREVAMMALLAKYHRHGLPTVDTFHGLLTQADVDRVRKLSALLRLAEYLERGR
                     TQVIQSITCRVRGKVVNLIAHTRGDATMEMWAANQNTDLFKQVFRHDLVIRLEPLTDR
                     DLSSTPIEPAGSADAEPLWSRVRELIEQK"
     gene            4096..4851
                     /locus_tag="VFF70_10660"
     CDS             4096..4851
                     /locus_tag="VFF70_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HZY45199.1"
                     /translation="MASLRKPNYPGKLFIVEGIDGSGKSTQLDLLRKWLISEGYLVAF
                     SEWNSSPVVQEATKRGKKQKLLTPTSFSLIHAADFSDRHTRQILPALEAGAIVLADRY
                     VFTAFARDVARGVHPEWVRDTYSFAVQPTVALYFRVPLEESLKRILVGRPALKYYEAG
                     LDLNLSPDPYESFRLFQDRILKEYDKLVDEYGLTVIDATLPLVKQQEHVRALIKPHLE
                     GAIKINPRAYRQLLAEDSLYGRYIDEIARSRRS"
     gene            4953..5753
                     /gene="tmk"
                     /locus_tag="VFF70_10665"
     CDS             4953..5753
                     /gene="tmk"
                     /locus_tag="VFF70_10665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZY45200.1"
                     /translation="MTALNFYGNGLPNLVDSVLPGKFIVIEGTDGVGRSTQIALLREW
                     LESRGYAVGSTGLSRSLLAGDAIKKAKDGHTLSESTLALFYATDLADRLEHEIIPALR
                     AGMVVLTDRYLYSIIARAQVRGVDARWARRMFGFALMPDAVFYLKADVAHLIPRVLGD
                     QGFDYWESGMDYLKGSSVFRNFVKYQTQLLAQFDAISEEFNFRVIDANRSVRETFYDL
                     RREIGAVVSDLKKQPIEEPAVPTDDVKAKEAEEGAEVKKDEVKESASS"
     gene            complement(5948..7714)
                     /locus_tag="VFF70_10670"
     CDS             complement(5948..7714)
                     /locus_tag="VFF70_10670"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZY45201.1"
                     /translation="MRTAENTLTPHEQTILHAITTQADPKISQRAQLILNWSIDPEPD
                     RLATATGWRPAQVKHWLQAFGQSRLNIFPPDVLWLASRGLDGSITVNQLCKKYKIDLG
                     HAKHVAQLSLMLFEHTQAIHQLPIERGRLLEAAAILHNAGMSIDQAQHHIVGRDIVIK
                     YKLTGFSDTERDSIACMVLFHRKKVKAEIDRLFATLAPDVQRETLILSALLRVGDGLD
                     YSTDQGTYIQSIDARPDRVDVQIDGPQAVSDAARAQHKSDLWQSIFKVPFTFSIGDQL
                     VELSQTARPHRVRRFGLKPTDLMAEASRSIMAQHFKRVRELELSARAGNEDAIHDLRV
                     AVRRLRVTVRLARSEYKQKSIAPLRSALSEFADRLAAVRDLDVILEHGLTFAQKHRSR
                     EAKLDLWLDHLKLRRAAAQNDLTRWLKSKRFHKFSSEFETFANTPQAAVRSKNDRLDS
                     ARVSEVMPSLIWEQYGAVRAYEAVRSMSIEQMHALRIEVKRLRDGLEFFSEVLGQETH
                     ELIDLLVRLQDHLGALQDAIVTQSLLAEYLIEQPKDAGALAYQATVDKEIKARRSQFP
                     EVWKPIINKPFRKLLARLLGEV"
     gene            complement(7872..8876)
                     /locus_tag="VFF70_10675"
     CDS             complement(7872..8876)
                     /locus_tag="VFF70_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HZY45202.1"
                     /translation="MIDLSTTYLGLNLKNPLVPSASPLSKSIDTLKRLEDHGASAVVM
                     YSLFEEQITHESRELDHYLSYGTDSYAEATTYFPDLANYNLGPQEYLNHIRQAKESLN
                     IPIIGSLNGVSNGGWVRYAKLIEQAGADALELNVYFIPTDPSISSTSVEQMYLDLVRH
                     VKASVKIPVAVKLTHFFSSLPYMAMRLDQVGADALVLFNRFYQPDIDLENLEVMANLT
                     LSSSYELRLRLRWAAILFGRIKADLAVTGGVHTAEDVLKSMMVGAKVAQMASALLHNG
                     MHVLDDILIELRRWLETHEYESIKQLQGSMSQRHVAQPAAFERANYMKVLNSFEVNVS
                     "
     gene            complement(9048..>10250)
                     /locus_tag="VFF70_10680"
     CDS             complement(9048..>10250)
                     /locus_tag="VFF70_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZY45203.1"
                     /translation="WDFFLNLPETDRATLNLTTVKDTQLLRPLFEFSLACMGCGETPY
                     LKLISQLYGDRAIIANATGCSSIYGGNLPTTPWAQNKEGRGPAWSNSLFEDNAEFGLG
                     MRLTLDKRIEFAHELIDRLRGSIGAELADQILQADQSSDQGINTQRDRIAQLKSKMKN
                     DQSVDAKNLISIADVLVEKLVWIVGGDGWAYDIGYGGLDHVLASGRNVNIIVLDTEVY
                     SNTGGQMSKATPRGAVAKFAAGGKPLAKKDLARLAMTYGTVYVAQIAMGANDAQTIKA
                     ISEAAAYNGPSLIIAYSHCIAHGINMTKGMEQQKLATVSGYWPLFRYNPDLAKAGHNP
                     LQIDSKAPAMPLEKYIYNETRYKMLTQSNPDVASELLKEAQADVNKKWKMYEQLAAVS
                     FNGDDTQK"
BASE COUNT         2209 a         2790 c         2854 g         2397 t
ORIGIN      
        1 gacaccggca tcaagttggg acttaatcat ccgatgggcc cgttcacgct gatggacttc
       61 atcggacttg atacctgcct gttcatcggc gatgcgatgt atgacgaatt caaagatgcg
      121 cgttacgccg cgccgccgct gctccggcga atggtcaccg cgggtttgat gggcagaaaa
      181 tcaaagcagg gtttctatca atattaaaat gttaatgctc ccgccgacaa cgggagtatt
      241 tttgtctata tcgacttcat tttgtggctc aaagcctcaa cactatgttt cgatcttctg
      301 ctcaacgtct tattgcaatt ctgatcgcct tactggccgt gccaacatta atcgccgccg
      361 cgcaatcgag cgagtggctg acgtactctg atccggttgc gggcttcgcc ttcgagtatc
      421 catcgaacgc gcatctgtcc accggcctcg atgcatcgca aggtaatagc agtgtgttcg
      481 tatcgatcgg cgcgggtcag gagtatcaag gttattcgat cgcggtgctg aacaacggca
      541 ggaatttagc gctcgatcaa ttcttgatcg agttgctgca cttggatacg gcgcagagca
      601 agcgcttaaa tctcaatggg cttgaggcga ttcaacagcc agccacgggg gccgatcagg
      661 ccgaagccta ttgggtgcag ctcgatcgcg tcgtgctgaa gatcgaactt tatcgcggcg
      721 atggatcgat cgagatcaca ccggattcgc gcgcggcgtt cgatcgagct ttagcgacac
      781 tccgatcgat cccgcagtct caaacgatcg ctccgtcatc gatcgcgccg aacgtgccga
      841 acatcggcat cgccgatcaa tttcaagcgc cgctcgcgac ggcgacgacg attcaatatg
      901 aaacgcagtg gaatgtgatt accagcgata cacgctatgg tgtgcgcaat ctagcgttga
      961 ccgggcgtaa atgctttggc gtagcgtgga atcggatgct gcacagcgcg atcgatctgt
     1021 atcgcttgga tggacaatcc gcggacggga caagcgtggt ggcggttgcg gatgggcaag
     1081 tggcttatta cgattcgagt tacgcttcgt atcccggcca tgtcgtgatc atccgccatc
     1141 aattagctga tagcagcctt atttattcgg tgtacgcgca catgaatgca gtctatattt
     1201 atcaaggaca gaatgtgatc cgcggccaac ccatcggcac ggtgctgtat caaccgaacg
     1261 attcgcatct gcattttgaa atgcgcaagt ttttggatgg cacttacatt tacacgggtt
     1321 atcctgcatg caatggcatc atctatggac gcggctacac ttatcaaatt caccccgata
     1381 attttccggc gcccaacggt tacatcaatc ccgacgcgtt cattcaagcg cacggcggat
     1441 cgccgctgac gccgatcggc ttgccggacg cttacgggcc gctgctgact tattcaacgt
     1501 tgaatgcttc aacgtcgatc gatcaagcgc agccgattac cgattcgcca tcgatgacgc
     1561 acaccgctta ctttccattg atcgtgcgcg agtatcctaa gcgcgaaccg gcgtgtgtcg
     1621 aaggccaaaa cttattgacg aacagcggct ttgaagatgg cgtcggtagc gcgccgtggg
     1681 tgcaggttcg caatagcaca gcggatagca tcgatgccaa ttggccctat tcaggcgtat
     1741 atagtgtttg gctgggtggc cgcaatctgg ccgatgagga agcgctgcaa tcgttcgtcg
     1801 tgccatacta caccgatggg atcacactga cgttcaaacg ctatctcaca actcaagaaa
     1861 cagctctcaa tactgtgtac gatcattttg aagtggtgct ggagaatacc gtcggcaatg
     1921 aaatctcgcc gcagctgcaa ttcagcaact tgagcacgaa gaatgtctgg atggcggaga
     1981 gcgcagcctt cgatggtgcg caggcttgga ccggacagcg attgcggctg agcatcaaag
     2041 ggatgacgga tggcagtctg ccatcgagtt tcttcgtcga tgaggtgagc gttcagacga
     2101 ggtgtgcgcc gtagcaaatg gcaaatgagg aagcccgatc gcgaccacga tcgggctttt
     2161 gttttgcagt aagtctttga gcttcagcta tgcgtcgatc gattaagcgg aatctggggc
     2221 tagttgcgtc cggttttgtt ctctccgata ttgacgaata ccgaggcgat gggttacact
     2281 atagtctaag aggacaagca tctatgcagc acaaaatcgg tatcatcgat ctgggctcaa
     2341 acaccagccg cttgatcatc atggcgtata cgccgggcgt tacctttcag ttgatcgatc
     2401 agctgcgcga gcgcgtgcga ttgagcgaag gcatgggcgc ggaaaatatt ttgcggcccg
     2461 aaccgatgga gcgcacgatc cgcttgctga aagtctttcg ccagttgtgc gaagcgaacg
     2521 gtatcgacac gattgtcgcg acggcgacca gcgccgcgcg tgatgcgcgc aatcaagccg
     2581 aatttttgga acgcgttcga actgaggccg gtttggattt acgcatcttg agcggcgccg
     2641 aagaagcgta ctatggttat ctcggcgcgg ttaacagcct gccgatccaa aacggcatgg
     2701 tggtcgacat cggcggcggc agcctggaat tggcccgcgt gcaagatcga cagttggcgc
     2761 aaacggccag tctgccttta ggcgcggtgc gcttgaccga aaccttcatc aagaacgatc
     2821 cacccaagag cctcgacctg cgtgtgatcg atcgctacat cgatgcgatg ctgggcagca
     2881 tcgattggtt gaaggccgga tcgggcggca tgctcgtcgg gttgggcggc actgcgcgga
     2941 cgctggccaa gatcgatcaa gagcgccgtc actatccgct cgatcgattg cacggttata
     3001 cgatttcgct aacttcgatc gaaaatattc tgcacgaact cgaacaactg ccgttgagca
     3061 aacgtgagaa agttcccggt ctgagcgccg atcgcgccga tgtgatcatc agcggcgcca
     3121 tcgccttgac gcgtttgatg catcaaggcg gttatcatga aatgacaatc tgcggtcagg
     3181 gtttgcgcga aggtttattc tacgaacagt tcttgaaaga ttccggcacg gctttggtgc
     3241 cggatgtgcg cgcgtttggt ttagcgaatc tgacgtatct ctacaacatc aactggccgc
     3301 atgcacgcca cgttcagcag ctagccctct cgatgttcga tcaacttcga tcgctgcatg
     3361 gctgcggccc gcttgagcga gcgatcttgt ccggcgcggc gctgctgcac gacgtcggtg
     3421 tggcgattga cttctacgat cacgatcaac attcctatta tttgatcttg aatgccgagc
     3481 tgcctggctt cagccaccgc gaagttgcca tgatggcgct gctggcgaag tatcaccgac
     3541 acggcctgcc cacggtcgat acattccacg ggctgctgac gcaagccgac gtagatcgcg
     3601 tgcgcaagct cagtgcgctg ctccgcctcg ctgagtattt ggaacgcggc cgcacgcaag
     3661 tgatccaatc gatcacctgc cgcgtgcgtg gcaaagtggt aaacttaatc gctcacacgc
     3721 gtggcgacgc cacaatggaa atgtgggcgg caaatcaaaa tactgatctc tttaagcaag
     3781 tctttcggca cgatctggtg atccgattgg aaccgctaac cgatcgggat ttgagctcaa
     3841 ccccgatcga gcccgctggc tcagcggacg ccgagccgtt gtggtcgcgg gtgcgggaat
     3901 tgattgaaca aaaatgaaag gtgcgtcatt gcgagcgaca tttgcaccgc actgcgtgct
     3961 tgtgcagtgc aggtgagtgc agcaatttca taatcgatgc tgagattgct gcgccgtggc
     4021 ccatccttag aagcttgtgc catctaagac tacgcggctc gcaatgacgt tgcagcacga
     4081 tcacaggagg ccaatatggc atcacttcgt aaaccgaact atccgggtaa actattcatc
     4141 gtcgaaggca tcgacggttc cggcaaaagc acgcagcttg atttgctgcg caagtggctg
     4201 atcagtgaag gctacttagt cgcctttagc gagtggaact cgtcaccggt ggtgcaggaa
     4261 gcgacgaagc gcggcaagaa acaaaaactt ttgacgccga cttcgttcag cttgatccat
     4321 gccgccgatt tttccgatcg acatacgcgg caaattctgc ccgcgctcga agctggcgcg
     4381 atcgtgttgg ccgatcgcta tgtattcacg gccttcgcgc gtgatgtggc gcgcggtgtt
     4441 catcccgaat gggtgcgcga tacctattca tttgcggtgc agccgacagt cgctttgtat
     4501 tttcgcgtgc cgttggaaga atcgctcaaa cgaattttgg ttggccgtcc ggcgttgaag
     4561 tattacgaag ccggactcga tctcaatctc agtcccgatc cctacgaatc attccgtttg
     4621 tttcaagatc gaattctcaa agagtacgat aaactcgtcg acgaatacgg cttgacggtg
     4681 atcgatgcga cgctgccgct ggtcaaacag caggaacacg tgcgcgcttt aatcaagccg
     4741 catctggaag gcgcgatcaa gatcaacccg cgcgcctatc gtcaactgct ggccgaagat
     4801 tcattgtatg gccgctacat cgatgagatc gctcgatcgc gcaggagtta agttcgggta
     4861 atcaatcgcg gcgatggctc ccatgccatc atttgattgg gcttgggagc ccaatcttca
     4921 atctgtcatg ttcatttgat tagaggttaa ctatgactgc actcaacttc tatggcaatg
     4981 gcctgcccaa tctggtcgat agtgtgctgc ccggaaaatt tatcgtcatc gaaggcactg
     5041 atggtgtcgg tcgatcgacg cagatcgcgt tgctgcgcga gtggctggag agccggggtt
     5101 acgccgtcgg ctcgacgggc ttgtctcgat cgctgttggc gggcgatgcg atcaagaaag
     5161 ccaaagacgg ccacaccttg agcgaatcga cgctggcctt gttttatgcg accgatctgg
     5221 ctgatcgttt ggagcacgag atcattccag cgctgcgcgc gggcatggta gtgctgactg
     5281 atcgctatct ctactcgatc attgcacggg cgcaggtgcg gggtgtcgat gcgcgctggg
     5341 cgcggcgcat gttcggtttt gcgttgatgc ccgatgcggt gttctatctc aaagccgatg
     5401 tggcccattt gatcccgcgc gtgctgggcg atcaaggttt cgattattgg gaatcgggca
     5461 tggattactt gaagggctcg agcgtttttc gcaacttcgt caaatatcaa acgcagctgc
     5521 tggcccagtt cgatgcgatc tcggaagagt tcaacttccg ggtgattgat gccaaccgat
     5581 cggtgcgtga gaccttttac gatctccggc gcgagatcgg cgcggtggtc agcgatctga
     5641 agaagcagcc catcgaagag ccggctgtcc caacggacga tgtaaaagca aaagaagcag
     5701 aagaaggtgc agaggtaaag aaggacgagg ttaaagagtc agcgtctagc taaacgaaga
     5761 atgggctccc acgcccaatc gcgcgatggc gtgggaaccg tcgctgcaat taaacttccc
     5821 ctaacaaacg cgccagcaat ttcatttgtc attcccgcga aggcgggaat ccagaatgtc
     5881 acaaactgga cacccgcttt cgcgggtgtg acgatggcca gcgaaggcga gggagccgtc
     5941 gctgcaatta aacttcccct aataaccgcg ccagcaattt gcggaacggc ttgttgatga
     6001 tcggtttcca tacttccgga aactggctcc ggcgcgcttt gatttctttg tcgacggtgg
     6061 cttgatacgc cagcgcgccc gcgtctttcg gctgctcgat caaatattcg gcgagcaggc
     6121 tctgtgtcac gatcgcatct tgcagcgcgc cgagatgatc ctgcaggcgc acgagcagat
     6181 cgatcaactc gtgcgtctcc tggcctaata cctcgctaaa aaactccagc ccgtcgcgca
     6241 gccgtttcac ctcgatgcgc agcgcatgca tttgctcaat ggacattgat cgaaccgctt
     6301 cataggcccg aactgcgccg tattgttccc agatcaagct gggcatgact tcactcacac
     6361 gggctgagtc aagtcgatcg tttttggatc gcacggcggc ttgcggcgtg ttcgcgaacg
     6421 tttcaaactc cgaggaaaat ttgtggaagc gcttgctctt caaccagcgc gtcagatcgt
     6481 tttgggccgc cgcgcggcgc agcttcaaat gatccagcca taaatcgagc ttggcttcgc
     6541 gcgatcggtg tttctgtgcg aacgtcaaac cgtgctcgag tatcacatcc agatcgcgca
     6601 cggccgccaa ccgatcggcg aactcgctca acgcgctgcg taaaggtgcg atcgatttct
     6661 gcttatactc cgatcgcgcg agccgtacgg taacgcgtaa gcgccgcacg gccacgcgca
     6721 gatcgtgaat ggcatcttcg ttgcccgcgc gggcgctcag ttccaattcg cgcacgcgtt
     6781 tgaaatgctg cgccatgatc gatcggctcg cttcggccat caagtccgtg ggcttcaagc
     6841 caaagcgtcg aacgcgatgc ggccgcgcag tttggctgag ctccacgagt tgatcgccaa
     6901 tcgagaaagt aaacggcact ttgaagatcg attgccacag atccgatttg tgctgcgcgc
     6961 gggcagcgtc gctcaccgct tgcggtccat cgatctgtac atccacccga tcgggcctcg
     7021 catcgatcga ttgaatatag gtgccttgat cggtcgagta atccaagcca tcgccgacgc
     7081 gcagcaaagc cgataggatc aaagtttcgc gttgaacgtc gggcgctaga gtggcaaaca
     7141 agcgatcgat ctcggccttg actttcttgc gatgaaacaa caccatgcag gcgatgctat
     7201 cgcgctcggt gtcggagaag ccggtcaatt tatatttgat cacaatatcg cgcccgacga
     7261 tgtggtgctg cgcttgatcg atcgacatgc cggcgttgtg aagtatcgcc gccgcctcaa
     7321 gcaggcgccc gcgttcgatc ggcagttgat ggatcgcttg cgtgtgttca aagagcatca
     7381 atgacaattg agccacatgt ttggcatgcc ccagatcgat cttgtatttt ttacacagtt
     7441 gattgacggt gatcgatccg tcaaggccgc gacttgccag ccataacaca tccggcggga
     7501 agatgttcag tcgagactgg ccgaacgctt gcagccaatg tttaacttgc gcgggccgcc
     7561 agccggtggc cgtcgccagt cgatcgggtt caggatcgat cgaccaattt agaatcaact
     7621 gggcgcgttg gctgatcttt ggatcggcct gggtcgtgat cgcgtgcagg atcgtttgtt
     7681 cgtgaggcgt aagagtattt tcggcagtgc gcatgtggct tctttggtga ggcttaccga
     7741 tcgcgattac attctaccac agcaagcttg tggcgtgccg gcacaagaga cagcggctga
     7801 tgagggtaag tcataagccg ctgtctgctg gggtttgatg atgtgccgct gctcaacagc
     7861 gaccgatctg tttagctaac attcacctcg aacgaattga gcaccttcat ataattcgcg
     7921 cgctcgaagg cggccggctg cgcgacatgg cgctggctca tgctaccctg cagctgcttg
     7981 atcgattcat actcgtgcgt ttccagccag cgccgcagct cgatcaggat gtcgtccagc
     8041 acatgcatgc cgttatgcag cagggccgag gccatctgcg ccactttcgc gccgaccatc
     8101 atcgacttca aaacgtcctc ggcggtatgc acaccgcccg tcacggccag atcggctttg
     8161 atgcggccga acaagatcgc ggcccagcgc aagcgaagac gcaattcata cgaactgctc
     8221 agcgtcaggt tcgccattac ttccagattc tccagatcga tgtcgggctg atagaatcgg
     8281 ttgaacaaga caagagcatc cgcgccgact tgatcgagcc gcatggccat ataaggcagc
     8341 gaactgaaga agtgcgtcag cttcaccgcg accggaatct tgacgctggc tttgacgtgc
     8401 cgcaccagat cgagatacat ctgctcgacg ctcgtgctgc tgatcgatgg atcggttggg
     8461 atgaagtaca cattgagttc cagtgcatcg gccccggctt gctcgatcag cttggcgtag
     8521 cgaacccacc cgccgtttga tacaccgttc aagctgccaa tgatcggaat atttaaagat
     8581 tcttttgctt gacgaatgtg attcagatat tcctgcggcc ccagattgta gtttgccaaa
     8641 tcggggaagt acgttgtcgc ttcggcgtag ctgtccgtgc catacgacaa atagtgatca
     8701 agttcgcggc tctcgtgcgt gatctgttct tcaaacagcg aatacatcac cacggctgat
     8761 gcgccgtgat cttccagccg cttcaacgtg tcgatcgatt tcgacaacgg cgaggccgac
     8821 gggacgagcg ggttcttcag attcaagccg agataggtcg ttgataagtc gatcattgtt
     8881 cgctccatta gtggcttctt gaaaatctga caaggtgacg ttcgctgttt gtcatttcaa
     8941 gcgcagcgaa aaatctcttt caatgatcgc agagatgctt cgtcccttcg ggactcagca
     9001 tgacaaataa agagatcacc ttgtcacccc ttcacccagt caccttgtca cttctgcgtg
     9061 tcatcaccat tgaaagacac tgccgctaac tgctcgtaca tcttccactt cttattcaca
     9121 tcagcctgcg cttctttcag cagctcgctt gcgacatcgg gattgctttg cgtcaacatc
     9181 ttgtagcgcg tttcgttgta aatatatttt tccaaaggca tggctggcgc tttcgaatcg
     9241 atctgcagtg gattgtggcc ggccttggcc agatcggggt tgtatctgaa cagcggccaa
     9301 tagccactga ctgtcgccaa cttctgctgc tccatgccct tggtcatgtt gatgccgtgc
     9361 gcgatgcagt gactgtacgc gatgatcaat gacgggccgt tgtaagccgc cgcttcgctg
     9421 atcgctttga tcgtctgcgc atcattcgcg cccatcgcaa tctgcgcgac atacaccgtg
     9481 ccgtacgtca tcgccaggcg cgccaaatct tttttggcta acggtttgcc gcccgccgca
     9541 aattttgcga ccgcgccgcg cggcgtggcc ttgctcattt gtccgcccgt gttggaatac
     9601 acctctgtat ccagcacaat gatgttgaca ttgcggcccg aggccagcac atgatcgagt
     9661 ccgccgtagc cgatgtcgta cgcccagcca tcgccgccga caatccacac cagcttctca
     9721 accagcacat cggcaatgct gatcaggttt ttggcatcaa cggactgatc gtttttcatt
     9781 ttcgatttga gctgcgcgat tcgatcgcgt tgggtgttga taccttgatc ggatgactga
     9841 tccgcctgaa ggatttgatc ggccagctca gccccgatcg atccacgcag ccgatcgatc
     9901 aattcgtgcg cgaactcgat ccgtttatcg agcgtgaggc gcatgcccaa accaaattcg
     9961 gcgttatctt caaacagcga gttgctccac gcggggccgc ggccttcttt gttctgcgcc
    10021 cacggcgttg tcggcaagtt gccgccgtag atcgatgagc agccggtggc attcgcaatg
    10081 atcgcgcgat cgccatacag ttggctgatc aatttgagat acggcgtttc accgcaaccc
    10141 atgcaggcca gcgaaaattc aaacagcggc cgcagaagtt gagtgtcttt gacggtcgtt
    10201 aaattcagcg tcgctcgatc ggtttcaggc aagttcaaga agaagtccca
//
LOCUS       DASRTJ010000527         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_261041,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000527 DASRTJ010000000
VERSION     DASRTJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261041"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1670)
                     /gene="pyk"
                     /locus_tag="VFF70_10685"
     CDS             complement(240..1670)
                     /gene="pyk"
                     /locus_tag="VFF70_10685"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZY45204.1"
                     /translation="MNRTKIVCTLGPACDDDDKIRAMIRAGMNVARINFSHGDHAAHL
                     TRIERVRRIAQEMKAIIAVLGDLQGPKLRVGEIANGFVMTYADREIILTTREVPGDEH
                     EVYLPHPDLVHDVKPGQRLLLDDGQLEFIVLGTTDTDIRCKVVNGGPLSSHKGVSAPG
                     ANLSLSALSDKDREDAVFGAEQQLDFLALSFVRTAEDVKKLRALLDAMGSKIPIVTKI
                     EKAEALENFSEILSCSDVIMVARGDLGVETPAESVPVVQKELIRHCNARGVPVITATQ
                     MLNSMITQPRPTRAEASDVANAIYDGTDALMLSAETASGKYPVESVEMMSKIAHITEQ
                     DVPFEKWSDRRVIGAAHSIADAIGHATVEIAYELNAKAIVTSTLSGNTTRLLARFRPR
                     AGIIAATPSEVVQRQLALVWGVTPILSQRYATTDDMIRVGVLLTVKNGLAQRGDTIII
                     TAGIPATSSNHTNMLKVHVIGEGDGQ"
     gene            complement(1752..>2504)
                     /locus_tag="VFF70_10690"
     CDS             complement(1752..>2504)
                     /locus_tag="VFF70_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY45205.1"
                     /translation="GLETLRDGIDISDEVFLQRLASSKQFPTTSQPPAGDFLEAFKKI
                     RGAGHEVLAVLVSNKLSGTVSSANTAKSELDNDAAITIFDTLNVAAGEGLMVLQAARM
                     AQAGQSQPEILAKLEHMRDHMHLLFVLDTLEYLAKGGRIGNAQKLIGSMLQMKPILEI
                     KHGAVEPFERIRTKVKARARLHEVVDHAVRGKSNVQLAVVYTEITFEATQVANELKEK
                     YHLRECNAYHMSPAVGAHAGPSALGIAYYFDA"
BASE COUNT          517 a          740 c          667 g          580 t
ORIGIN      
        1 gcgttggaat gtctgtccgt attgcggcac tccggccatg gcgggcgtgc agcttcaagc
       61 gactgaggcg aatgcggcgt tgaagcggcc tgctcgacgg actgaagagg ttgtcgaagc
      121 gccgccgcca gcgcaagcct aatcatccaa aatagaaacc aggtttctta tagaaacctg
      181 gtttcttttt agctatactc gcaccgtagt tcaagtgtgg caaactcttt gaaacatgat
      241 tattgcccat cgccttcgcc gatgacatgc actttcagca tgttcgtgtg gttgctcgac
      301 gtagcgggaa tccccgctgt gatgatgatc gtatcgccgc gctgagcgag tccgttcttc
      361 acggtcagca ggacgcccac gcggatcatg tcatcagtcg tagcatagcg ctgcgataga
      421 atcggggtaa cgccccagac caatgccagt tggcgctgca ccacttccga tggagttgcg
      481 gcgatgattc cggcacgcgg gcggaagcgc gccagcaagc gcgtcgtatt cccgctaagg
      541 gttgaagtga caatcgcttt cgcgttgagc tcataagcga tctcgaccgt ggcgtgaccg
      601 atcgcatcgg cgatgctgtg cgccgcgccg atcacccgtc gatctgacca cttctcgaac
      661 ggcacatcct gctcggtgat atgcgcgatc ttggacatca tttcgaccga ttcgaccgga
      721 tatttaccac tggccgtttc ggccgatagc atcaaggcat cggtgccatc atagatcgca
      781 ttcgccacat cgcttgcttc ggcgcgtgtc ggtcgaggtt gagtgatcat cgagttcaac
      841 atctgcgtcg ccgtgatgac tggcacaccg cgcgcattgc aatgccggat caactctttc
      901 tgaaccaccg gcacgctttc ggccggagtc tctacgccga gatcgccgcg ggcgaccatg
      961 atcacatcac tgcaactcaa tatctcgcta aagttctcca gcgcctcggc cttttcgatc
     1021 ttcgtcacga tcggaatttt tgagcccatc gcgtcgagta aggcgcgcag cttcttcaca
     1081 tcttcggcgg tgcgcacgaa cgataaagcc agaaagtcca actgctgttc tgcgccaaag
     1141 acagcgtcct cccgatcttt gtccgataag gccgacaagc tgagattggc accgggtgca
     1201 ctgacacctt tgtgcgaact caacgggccg ccattgacaa ccttgcagcg gatgtccgta
     1261 tcggttgtac cgaggacgat gaattccaac tgtccatcgt ccagcagcag acgttgaccc
     1321 ggcttaacat cgtgaaccag atcggggtgc ggcagataaa cttcgtgttc atcgccggga
     1381 acttcgcgcg tggtcaggat gatctctcga tcggcatacg tcatcacgaa tccgttcgcg
     1441 atttcgccga cccgcaattt cggaccctgg agatcaccca ggacggcaat aatcgccttc
     1501 atttcttggg cgatgcgccg cacccgctcg atgcgcgtca aatgcgcggc gtgatcgccg
     1561 tgcgagaagt tgatccgcgc cacattcata cccgcccgga tcatcgcgcg gatcttgtcg
     1621 tcgtcatcgc aggccgggcc taacgtgcac acaattttgg ttcgattcat gttctggtcc
     1681 ttagctaaaa aacgagggag cgctgcccgc tccctgtttg aatgaacttg gtcctgcgtc
     1741 gatcgctgcg actaagcatc gaaataataa gcgatgccca acgcgctcgg cccggcatgc
     1801 gcgccgacgg ccgggctcat gtgataggca ttgcattcgc gcaaatgata tttttctttc
     1861 aactcattgg caacttgcgt agcctcaaac gtgatctccg tatacaccac ggccagctgc
     1921 acgttcgatt taccgcgcac ggcatgatcg accacttcat gcaagcgggc acgggctttg
     1981 accttcgtac ggatgcgctc aaaaggctcg accgcgccgt gtttgatttc gaggatcggt
     2041 ttcatttgca gcatcgaacc gattaacttc tgggcattgc caatgcggcc gcctttcgcc
     2101 aaatattcca gcgtatccaa cacaaagagc agatgcatgt gatcgcgcat atgctcgagc
     2161 ttcgccaaaa tttctggctg gctctggccg gcttgtgcca tgcgcgccgc ttgcagcacc
     2221 atcaggcctt cccctgccgc cacattcaag gtgtcgaaga tcgtaatcgc cgcgtcgttg
     2281 tccaattccg atttggcggt attggccgat gagaccgtcc cgcttaattt gtttgagacc
     2341 aacacggcca acacttcatg gcccgcgccg cgaatttttt tgaatgcttc cagaaaatcg
     2401 cccgcgggcg gctgtgaagt tgtcggaaac tgtttggaac tcgccaagcg ttgcagaaat
     2461 acttcatcgg aaatatcgat tccatcccgc agtgtctcga ggcc
//
LOCUS       DASRTJ010000528         8912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_71632,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000528 DASRTJ010000000
VERSION     DASRTJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71632"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..907
                     /locus_tag="VFF70_10695"
     CDS             116..907
                     /locus_tag="VFF70_10695"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HZY45206.1"
                     /translation="MNKLAKFESLAGQWVEGTFSRLLGGRLQPMEVATALARAMEDEQ
                     FTADTGDQFAPNVYWVYLNQADYAALRHTQPTLPSDLASSLIDLAERGGLKMPDRPIV
                     EIRSDPAIPARHISVAAKYMAQDTAPISATDEIAQDALDLARSAPKTAMTHSFLILEG
                     RRHVALSKPVITLGRALDNDIVLDDTRVSRHHAQLRIRQGQYILYDTGSSGGSSVNGG
                     PVSEVLLRGGDVISLAGVEIIFGIDDFSATQPASAPDDTPPMQPH"
     gene            912..1388
                     /locus_tag="VFF70_10700"
     CDS             912..1388
                     /locus_tag="VFF70_10700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY45207.1"
                     /translation="METAIFVFRILLSSLLYVFLATLFVFLWRDLKSVTQPQMTTSVR
                     ERPAQLRVIHGSNGLIENEICALSAYTSIGRSAKNSIKIAETYASAEHALIVWRNTQW
                     WLEDRSSRNGTLLNDVPITAPLIISQGDVIGIGQLRLKFEYLNELDNGKNGSSTQG"
     gene            1366..2166
                     /locus_tag="VFF70_10705"
     CDS             1366..2166
                     /locus_tag="VFF70_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY45208.1"
                     /translation="MDLRLKDKVVLVTAASRGLGAASAHRFAAEGAQVAISARDKSHI
                     TQTALDIAKDTAARIFPIVGDVTKPEDADRTVQDVIDRFGRIDILIVNAGGPPAGPFK
                     DMKWDQWEAAAHLVLLSAVRLCQAAIPHMLKNNPPDRGSIVAITSYVARQPGDGLAIS
                     NSLKISVVGLMKSLANEYAAQGIRVNSIAPGWTMTDRIKDIFRNRSERSGTSFDQEAA
                     KVIAQIPIGRIANPDEFADALVWLASPCASYVTGILLPVDGGLTQSYL"
     gene            2175..2651
                     /locus_tag="VFF70_10710"
     CDS             2175..2651
                     /locus_tag="VFF70_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HZY45209.1"
                     /translation="MNKRVELIEQIKSGREELDGLLNQLSDEKMLVAALPGNWSIKDL
                     LAHFEWWAKRAANLYRFLSRGQTPPDEDIEIDQLNARVYAENQNRSLDNVRREEREAY
                     QALLALATEAPEADLFEPHRFAWTQGNPFVNWIINNSYSHFAEHLGDVQAWLKANQ"
     gene            2630..4675
                     /gene="ligA"
                     /locus_tag="VFF70_10715"
     CDS             2630..4675
                     /gene="ligA"
                     /locus_tag="VFF70_10715"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZY45210.1"
                     /translation="MAEGKPVTANLQKRLAELREQINFHSYRYHVLDDPIISDAEYDR
                     LLQELIKIETEHPEWITPDSPTQRAGAQASEKFPKVPHPAPILSLANAFNGDDVRTWY
                     ARISKLIPEGRKVGFVVEPKIDGLTVILQYRDGVFAQGATRGDGSIGEDVTANLRTVR
                     SVPLKIPVAKDGKTKSSINIPKRLVVRGEAFIETKAFEAMNKQIEKEGGKTFANPRNA
                     GAGFLRQLDPSITAKRPISLLCYAIVAADSATARSQWELLEYLSAIGFPVTKVAKKFD
                     SLDKAIAYCESWFDKRDELSFEADGMVIKIDDLDLQAELGSVGKDPRGAIALKFPARE
                     VTTQLIDLGVNIGRTGVMAPYAILEPVNIGGVTVERATLHNFDDIARKDIRIGDRVIV
                     KRSGDVIPYVSGPVVVVRTGKEKKIEPPKKCPFCDTPIVRRAGEVAIYCPNRNCPGKL
                     DRAIQFFVAVMDIEGMGEKIASQLIEVGLVENVADIYSITRDQLLQLEGFADKKADKL
                     LESIKISKTRSLDRLIAALGIPHVGGVAAESLAAHFGSIDVFLKATEDDLTQIEGIGP
                     TIAASIVEWTQRRTNKNLIARLKKEGINPKIDRATQKPSDGKLSGKTFVITGTLSMPR
                     DEIASMIKFAGGKVIDSVSPKTDYVVVGDSPGSKYSKAQELGVIILDEAALAKLLKG"
     gene            4892..6079
                     /locus_tag="VFF70_10720"
     CDS             4892..6079
                     /locus_tag="VFF70_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HZY45211.1"
                     /translation="MDSIEARIKFWRERVDRTHADDTYVYQRPIDSIDGAYVSVHGRR
                     MLMLASYSYLGLPGHPEITQAAQAAAQKYGTGTHGVRLLAGTLDLHQELEQTIARFKH
                     AADAVVFSSGYVTNLTTIATLLGRNDVVICDKLDHASIVDGCLLSGAKFLRFKHSDPN
                     DLERQLQAAGDRSKLVIVDAVFSMDGDIAPLPEISRLCKQYTAWLMVDEAHSIGLLGS
                     TGHGIEEHFDLPPDTIDIKMGTLSKCIPSVGGYIAGSNDLINYLQHVAHAYIYSAALP
                     PAQAAAAKCAFEIIEREPDRVQRAQANGHRLRTGLNSLGFDTLHSTTAIVPLMTCNED
                     RALHMAALCQQRGYFVLPVIAPAVPSELSRLRVTVTAAHTSSNIEQALDIFKQTGKEL
                     GVI"
     gene            6079..7254
                     /locus_tag="VFF70_10725"
     CDS             6079..7254
                     /locus_tag="VFF70_10725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZY45212.1"
                     /translation="MTLGKVALKLNRDRPIKQHHPWIFSGAIDRIDPSIADGDIADIV
                     SAQGEFLARGYVNRRSQIVVRLLTWAASESIDQDFWVRRLTRSISARPTIPARRLVNA
                     EADGLPGLIVDQYNEFSVAQFLTLGMDRHKQQIADALNGLLNPTGLYERSDVDIREKE
                     GLERVTGLLRGVEPPDKMKIDENGWQFWVDAKHGHKTGWYLDQRDNRRKIQTYCQNAE
                     VLNLFSFTGGFSVSALTGGAKSVINVDSSAASLELAKENYRLNGLSLDDQDFVIADVF
                     SYSRKLRAEHRSFDVIIADPPKLAQSQAQLEKATRAYKDLNLISMQLLKPGGTLITFS
                     CSGLVSPDLFQKIIFGASIDAGRDVQIVEKLSQAGDHPILLSFPESEYLKGLVCRVI"
     gene            7239..7721
                     /locus_tag="VFF70_10730"
     CDS             7239..7721
                     /locus_tag="VFF70_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY45213.1"
                     /translation="MPRDLSKHWDVGVTGAVVLDRKVLFVQRNYEPNKGTWTLPGGYA
                     EHTETLDDAVKRELKEETGIEIEVKGVVGVRTRYGDNGGAVLVIFRAEIRSGVPHADM
                     HEISATEFFDADQIAKLEPVFPLSREVGLRVLESDSDGLIEREIPSSLNSIKWKAFTI
                     "
     gene            complement(7791..8384)
                     /locus_tag="VFF70_10735"
     CDS             complement(7791..8384)
                     /locus_tag="VFF70_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZY45214.1"
                     /translation="MNVAAIILAAGQAKRMGSNKMLLPFGRSTVIEAIVTEVVVCNLS
                     DVVVVTGHEHEQIATLLAKYSVRCVFNSGYDRSEMIVSIQAGLRALSEKIQAALIVLG
                     DQPRLQRDVVQRIVEAYAPHSLIVPSYQNRRGHPILIDRSLWADVLALPSTATLRDFV
                     QSHAAHIRYIELDSDSIMHDLDTPQDYQGLIHREDVK"
     gene            complement(8474..8650)
                     /locus_tag="VFF70_10740"
     CDS             complement(8474..8650)
                     /locus_tag="VFF70_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45215.1"
                     /translation="MIPLLPIDLLARSIPATELGFARLVVMSAWYPTVGAVYSICASY
                     LTSICALQNIRVCP"
     gene            complement(8804..>8912)
                     /locus_tag="VFF70_10745"
     CDS             complement(8804..>8912)
                     /locus_tag="VFF70_10745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZY45216.1"
                     /translation="RLTIPSDLGYGAQGAGNSIPPNATLIFEVELVSIK"
BASE COUNT         2153 a         2378 c         2415 g         1966 t
ORIGIN      
        1 cgatccgatc ggggtaagat ataatcgcgc aggtaggaca agtgcctaac ttgccccaat
       61 aagtaagttg tcaaggaaca tgcagccgac tttttcgaca agtcatgagg caattttgaa
      121 taagctagcc aaattcgaat cactcgcggg tcaatgggtc gagggaacat tcagccgctt
      181 gctcggcggc cgcctacaac cgatggaagt cgccacagct ctggcgcggg cgatggaaga
      241 tgaacaattt acggctgaca ccggcgatca attcgcaccg aatgtttatt gggtctatct
      301 gaatcaagcc gattacgctg ccctgcgcca tacgcagcca acactaccga gcgatctggc
      361 cagctcgttg atcgatctgg cggagcgcgg cggtttaaaa atgcccgatc ggccgatcgt
      421 ggaaatccga tcggacccag cgatccccgc gcggcatatc tcagtagccg caaagtatat
      481 ggcgcaggat actgccccca tcagcgcgac ggatgagatc gcgcaggacg cgctcgattt
      541 agcgcgctcg gcgccgaaga cggcgatgac ccatagtttc ctgatccttg aaggtcgccg
      601 acatgttgca ttgagcaagc ccgtcatcac acttggccgc gcgctggata atgacattgt
      661 gctcgatgat acacgcgtgt cgcgccatca cgcccaactg cgcatccggc aaggccaata
      721 cattttatat gatactggct ccagcggcgg atcgagcgtg aacggcgggc cggtatctga
      781 agtgctgttg cgcggcggcg atgtgatctc gttagcgggc gtagaaatta tcttcggcat
      841 cgatgacttc agcgcaactc aaccggccag cgcgccggat gacacacctc ccatgcagcc
      901 tcattgaaat tatggaaacc gcgatttttg tcttccgcat tctactcagc agcttactgt
      961 atgtcttctt agctacactc tttgtatttt tatggcgcga tctcaaatca gtgacccaac
     1021 cacaaatgac cacgagtgtg cgcgaacgtc ctgctcaact gcgtgtcata cacggcagca
     1081 atgggctgat tgagaatgaa atttgtgcac tatccgccta tacctcgatc ggccgatcgg
     1141 ctaagaactc gatcaaaatt gccgagacgt atgcatcggc cgaacatgcc ttgatcgtct
     1201 ggcgcaacac tcaatggtgg ctggaagatc gatcgagccg caatggcacg ctgctcaacg
     1261 atgtgcctat caccgcgccg ctcatcatca gtcaaggtga tgtgatcggc atcggccagc
     1321 tgcgcttgaa attcgaatac ctcaacgaat tggacaacgg gaaaaatgga tcttcgactc
     1381 aaggataaag ttgtgttagt gactgccgcg tcacgcggtt tgggcgcagc cagcgcgcac
     1441 cgtttcgccg cagagggcgc tcaggttgcc atcagcgcgc gcgataagtc acatatcacg
     1501 cagactgcac ttgatattgc caaagacact gctgcacgga tatttccaat cgtcggcgat
     1561 gtcactaagc ccgaagatgc agatcgaacc gttcaggatg ttatcgatcg tttcgggcgc
     1621 atcgatattt tgatagtcaa tgcgggcggc ccgccggccg gcccattcaa agatatgaaa
     1681 tgggatcaat gggaagcggc ggcccacctc gttttgctga gcgccgtgcg cttgtgccag
     1741 gcggctattc cgcacatgct taagaacaac ccgcccgatc gcggatcgat cgtggcgatc
     1801 acgtcgtatg tggcccgcca gccgggcgac ggcttggcta tttcaaactc gctcaagatt
     1861 agcgtcgtcg gcttgatgaa atcgcttgcg aatgaatacg cggcgcaagg cattcgagtt
     1921 aactcgatcg cgccaggctg gaccatgacc gatcggatta aagatatttt tagaaatcga
     1981 tcagagcgca gcggcacatc gttcgatcag gaagccgcta aagtgatcgc ccaaatcccg
     2041 atcggccgta tcgctaatcc cgatgaattc gccgacgcgc tcgtatggct ggcttcgccg
     2101 tgcgcatcgt acgtcaccgg cattttattg cccgtcgatg gcggcctcac ccaaagttat
     2161 ttataggaga tacgatgaac aagcgagttg aactcatcga acaaataaag tcagggcgtg
     2221 aggaattgga tggcctgctc aatcagctca gcgacgagaa gatgttggtc gctgcgctgc
     2281 cgggcaattg gtcaattaaa gatctgttgg ctcattttga gtggtgggct aagcgcgcag
     2341 cgaatttata tcgattcttg tcacgcggcc aaactccgcc ggacgaagac atcgagatcg
     2401 atcaacttaa cgcccgcgtt tatgccgaga atcaaaatcg atcattggat aatgtgcgcc
     2461 gtgaggagcg cgaagcctat caagcactgt tggcacttgc taccgaagcg ccggaagccg
     2521 atctgtttga accacatcgt tttgcgtgga cgcaaggcaa tccgttcgtc aattggatca
     2581 ttaataactc ctactcgcat tttgcagaac accttggaga tgtacaggca tggctgaagg
     2641 caaaccagta acagccaatt tacaaaaacg cttggccgaa ctgcgcgaac agattaattt
     2701 tcattcctat cgttatcacg tactcgacga tccgatcatc agtgatgccg aatacgatcg
     2761 cttgctgcaa gagttaatca agatcgaaac cgagcatccc gaatggatca ctcccgactc
     2821 acccactcaa cgcgcgggcg cgcaggccag tgaaaaattt ccgaaggttc ctcatcccgc
     2881 gccgatttta tctttggcaa atgcgttcaa cggcgatgat gttcgaactt ggtatgcacg
     2941 catctcgaag ttaattcctg aaggccggaa agtcggtttc gtcgtcgaac cgaagatcga
     3001 tgggctcacc gtgatcttac aatatcgcga cggtgtcttc gcgcaaggtg caacccgcgg
     3061 cgatggctca atcggtgagg atgtcacggc gaatttgcgc accgtgcgca gcgtaccgct
     3121 caagatcccg gtcgcgaaag acggtaagac gaaatcatcg attaacattc ccaaacgctt
     3181 ggtggtgcgc ggcgaagcct tcatcgagac caaagctttt gaagcgatga acaagcagat
     3241 cgaaaaagaa ggcggtaaga cctttgcgaa tccgcgcaac gcgggcgcgg gctttttgcg
     3301 tcagctcgat ccgagcatca cagcgaagcg gccgatcagt ttgctgtgct acgccatcgt
     3361 cgctgcggac agcgcgacgg ctcgatcaca gtgggaactc ttagaatact tgagcgcgat
     3421 cggttttcct gtgacgaagg ttgcaaagaa attcgattca ctcgataaag cgatcgcgta
     3481 ttgcgaatcg tggttcgata agcgcgacga gttatccttc gaagcggacg gcatggtgat
     3541 caagatcgat gatctcgatc tgcaagccga actcggttcc gtcggcaaag atccacgcgg
     3601 cgcaatcgcc ttgaagtttc cggcgcgtga agtcacaaca cagctgatcg atctcggcgt
     3661 caacatcggc cgtaccggcg tgatggcacc gtacgcgatc cttgagccag tcaacatcgg
     3721 cggcgtcacc gtcgagcgcg caacactgca caacttcgat gacatcgccc gtaaagatat
     3781 tcgcatcggc gatcgcgtca tcgtcaaacg ctccggcgat gtgattccgt acgtgtccgg
     3841 tccggtcgtg gtggtgcgca caggcaaaga gaaaaagatc gagccgccaa agaagtgtcc
     3901 gttctgcgat acgccgatcg tccggcgcgc aggcgaagtg gcgatctact gcccaaatcg
     3961 aaattgtccc ggtaaactcg atcgagcgat ccagttcttc gtcgccgtga tggacatcga
     4021 agggatgggc gagaagatcg catcgcaatt gattgaggtg ggtttagttg aaaacgtggc
     4081 tgacatttat tcgatcacgc gcgatcagtt actgcagctc gaaggctttg ccgataagaa
     4141 ggccgacaaa cttttagaat cgatcaagat ttccaaaacc cgatcattgg accgattaat
     4201 cgcggcactc ggcattccgc acgtcggcgg cgtggcggct gaatcgctcg ccgcgcattt
     4261 cggatcgatc gatgtctttt tgaaggctac cgaagatgat cttactcaga tcgaaggcat
     4321 cggcccgacg atcgccgcga gcatcgttga atggacacag cgcaggacga acaagaactt
     4381 gatcgcacgg ctgaagaagg aaggcatcaa ccccaagatc gatcgggcga cgcaaaaacc
     4441 atcggatggc aaactaagtg gaaaaacctt cgtcatcacg ggcacattat cgatgccgcg
     4501 tgatgagatc gcatcgatga tcaaattcgc tggcggcaaa gtgatcgaca gtgtcagccc
     4561 aaaaaccgat tacgtcgtcg tcggcgattc gcccggcagc aaatattcta aagctcaaga
     4621 attgggcgtc attattttgg acgaagcggc gctagcgaaa ttgttgaaag gctagctgtc
     4681 cttgcgagga gtgcgtagca cgacgaagca atctcgttga tgatcgctga gattgcttcg
     4741 tcgcaaaaga cgctcaccta ccctgtccgg tgttgacagg gtcgcaatga tgatcggatc
     4801 gctcttcatc cctcagcctt caacctttct ttgacttccc tgacgcgcga tgatacactc
     4861 cgcgcgtcaa tattttgtcg ggagcttaag tatggattcg attgaggcgc gcatcaaatt
     4921 ttggcgcgag cgcgtcgatc gaacgcacgc ggacgacacc tacgtttatc aacgaccgat
     4981 cgattcgatc gacggggcgt atgtcagcgt ccacggccgg cgcatgttga tgctggcttc
     5041 atatagttac ctcggcttac cgggacatcc agaaattacg caagccgcac aagccgccgc
     5101 gcaaaaatac ggcaccggca cgcacggcgt ccgcttgtta gccggcacac tcgatctgca
     5161 ccaggaactc gaacaaacca ttgcgcgctt caagcacgcc gccgacgccg tcgttttttc
     5221 cagcggctat gtaaccaatt tgacgaccat cgccacgctg ctgggccgca atgatgttgt
     5281 catctgcgac aaactcgatc acgccagcat cgtcgatggc tgcttgttaa gcggcgcaaa
     5341 gtttttgcgc ttcaagcaca gtgatccaaa tgatctggag cgccaacttc aagcggccgg
     5401 cgatcgatcg aaactggtga tcgtcgatgc agtcttcagc atggatggcg acatcgcgcc
     5461 actgcctgag attagtcgat tgtgcaagca gtacaccgcc tggctgatgg tggatgaagc
     5521 tcactcgatc ggtttgctcg gctcgacggg gcatggcatc gaagaacatt tcgatctgcc
     5581 gccggacacg atcgatatca agatgggcac gctctccaaa tgcattccaa gcgtcggcgg
     5641 ttatatcgcc ggatcgaacg atttgatcaa ctacctgcag catgttgcgc acgcttacat
     5701 ttattcagcg gcgctgccac ccgcgcaagc cgccgccgcc aaatgcgcat tcgaaatcat
     5761 cgagcgcgaa cccgatcgcg tgcagcgcgc gcaagccaac gggcacaggc tgcgcactgg
     5821 cttgaactcg ctcggatttg acacgctgca cagcaccact gcgatagtgc cgctcatgac
     5881 gtgcaatgaa gatcgcgctc tgcacatggc ggcgctctgt cagcagcgcg gctattttgt
     5941 tttgccggtc atcgcgcccg cggtcccctc cgagctcagt cgcttgcgtg tcaccgtcac
     6001 ggcggcgcac acttcgagca acattgaaca agcgctggat attttcaagc agacgggcaa
     6061 agaattgggt gtgatctaat gacgcttggt aaagttgccc tcaagttaaa tcgcgaccgg
     6121 ccgatcaagc agcatcatcc gtggattttt tccggggcga tcgatcggat tgatccatcg
     6181 atcgccgatg gtgatattgc ggatatcgtt tcagcgcagg gggaattcct ggcgcgcggc
     6241 tacgtcaacc gtcgatcgca gatcgtggtt cgattgttga cgtgggctgc atcagaatcg
     6301 atcgatcaag acttttgggt tcggcgctta acccgatcga tctcggcacg cccgacgatc
     6361 ccggcgcgcc gcttagtcaa cgccgaagcg gatggattgc ctggcttgat cgtcgatcaa
     6421 tacaacgaat tcagtgtggc gcaatttctt actttgggaa tggatcgtca caagcagcaa
     6481 attgccgatg cacttaacgg cctactcaac cccacgggtc tgtatgagcg cagcgacgtc
     6541 gacatccgcg aaaaggaggg actggagcga gttactggat tactgcgtgg cgttgagccg
     6601 ccggacaaaa tgaaaattga tgagaatggt tggcaattct gggtggatgc taaacacggc
     6661 cataaaacag gctggtatct cgatcagcgc gacaatcgac gcaaaattca aacctactgc
     6721 caaaatgccg aagtcttgaa tcttttttcg tttacgggcg gtttctcggt gtcggcgtta
     6781 acaggagggg caaagtctgt tatcaacgtc gattcgtctg cggcctcatt agagttagcc
     6841 aaagaaaatt atcgtttgaa tggactcagc ttggatgatc aagattttgt tatagcagat
     6901 gtcttctcgt atagtcgcaa gctgcgcgcc gaacatcgct cattcgatgt catcatcgcc
     6961 gatccgccga agttagccca atcgcaagcg caacttgaaa aagcgacgcg cgcttataaa
     7021 gatctgaatc tcatctcgat gcagctgctt aagccgggcg gcaccttgat caccttctcg
     7081 tgctccggcc tcgtctcgcc cgatctgttc cagaagatca tcttcggcgc atcgatcgat
     7141 gccggccgtg atgtgcagat cgtcgagaag ttgtcgcaag cgggcgatca tccaatcttg
     7201 ttgagctttc cggaatcgga gtatttgaaa ggcttagtat gccgcgtgat ttgagcaaac
     7261 attgggacgt cggtgtaacg ggcgcagttg tgcttgaccg gaaagtctta ttcgttcaac
     7321 gcaactacga accgaataaa ggcacgtgga ctttgcccgg cggttatgcc gaacatactg
     7381 aaacgcttga tgatgccgtc aagcgcgaac tcaaagaaga gaccgggatt gagattgagg
     7441 tcaaaggcgt ggtgggagtg cggacgcgtt acggagacaa tggcggcgcg gtcttagtga
     7501 tcttccgcgc cgaaattcga tcgggcgtgc cgcacgccga tatgcacgag atcagcgcga
     7561 cggaattctt cgatgccgat cagatcgcga aactcgagcc agtgtttcca ttatcgagag
     7621 aggttggcct gcgtgtactt gaaagcgatt cggatggatt gattgagcga gagattccgt
     7681 ccagcttgaa ttcgatcaag tggaaagcat tcacaattta atgaacaagg agcaattatc
     7741 aattttcgat gagattgctt cgccgctatg cggctcgcaa tgacgtatcg ctacttaacg
     7801 tcttcgcgat gaatcagtcc ttgataatct tgtggggtat ccagatcatg catgatgcta
     7861 tccgaatcca attcgatata ccgaatgtga gccgcatggg attggacgaa gtcgcgcaga
     7921 gtggcagtcg acggtagggc tagcacatca gcccacagcg atcgatcgat cagaattgga
     7981 tgcccgcgtc gattttgata cgagggaaca atcagagagt gcggcgcgta cgcttcgacg
     8041 attcgctgca ccacatcgcg ctgaagccgc ggctgatcgc ccaacacgat caacgcagct
     8101 tggattttct ccgacaacgc gcgcaagccc gcttggattg agacaatcat ctccgatcga
     8161 tcgtaaccag aattgaagac gcaccgcacc gaatattttg ccaggagcgt ggcgatctgt
     8221 tcatgctcgt gaccggtgac aaccacgacg tcgcttagat tgcacaccac gacttcagta
     8281 acgatcgctt caatcacggt cgatcgacca aagggcagca gcattttgtt gctgcccata
     8341 cgtttcgctt gtccagcagc aaggatgata gcagcaacat tcatttactt agaggctccg
     8401 cttgttcgag cgcgcgcttc gcgctcgact caggatgctt gatcgtcgaa tgattgatag
     8461 cccacacaac gcatcacggg cagacgcgga tattttgcaa agcgcagatc gatgtcagat
     8521 aactggcaca gatagaataa actgccccga ccgttggata ccatgcggac atgacgacaa
     8581 gtctggcaaa gcccaattcg gtcgcgggga tcgatcgggc tagaagatcg atcggcagta
     8641 acgggatcac tcatcgcgtt tcattataga tcgaaatcga gactccagca gaggaaaata
     8701 aaaccatgca aaggttgttg aatcgatcga tgacattcaa ttcaaccttc gcaaggtttc
     8761 acaacgttcg caacccttcg acagcgctca gggcgggcgt gtcttacttg atcgaaacta
     8821 attcgacttc gaagatcaat gtcgcattcg gtggaatcga attgcccgca ccttgcgcgc
     8881 catagcccaa atccgacggg atcgtcaagc gg
//
LOCUS       DASRTJ010000529         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_108620,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000529 DASRTJ010000000
VERSION     DASRTJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108620"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFF70_10750"
     CDS             complement(<1..449)
                     /locus_tag="VFF70_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HZY45217.1"
                     /translation="MDKLDSIIEAIRSKFTERNTARDLTLNRSRELIRYCSLTIRATH
                     REEFDEAEKLLASAKQFAAEMKADIAEHPALYYTGYTQDALKELTEACVVYAIVRDRS
                     IPSHTEIEVDEAAYLNGLAEAASELRRRCLDLIRQERNAEAERMLTA"
     gene            complement(453..791)
                     /locus_tag="VFF70_10755"
     CDS             complement(453..791)
                     /locus_tag="VFF70_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004916801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZY45218.1"
                     /translation="MSIPSNPLPQSNNPTPINIEIPNELKAHYANFALIMHTPSEVIL
                     NFTSVLPGMTKAQIDTRIVMTPLHAKLMLNALADNLQKYETQFGEIKLPPGGTGLAEQ
                     LFGGVKPTGS"
     gene            complement(948..1376)
                     /locus_tag="VFF70_10760"
     CDS             complement(948..1376)
                     /locus_tag="VFF70_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZY45219.1"
                     /translation="MYQQIVLVGNLGRDPEMRYTPSGTPVTSLSVATNRKYNDKDNQQ
                     VKETTWFRVSVFGKQAEACAQYLKKGSSVLVEGRLTPDPTTGGPRVFTRQDGSTGTSF
                     EIFANTVRFLGGRDAAGGTAGPSGAPEDVAAAGGEEEIPF"
     gene            1668..>2696
                     /locus_tag="VFF70_10765"
     CDS             1668..>2696
                     /locus_tag="VFF70_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45220.1"
                     /translation="MSQLDSITSPLTPAYEPLAPRSIEETGLNLGFLTELALKIMYFE
                     GYLSGSQIADRMKLPFTGVVDQVLEFLKREQMAEVKGSGGFGEQAYQYAISMKGREKA
                     REALDRSQYAGAAPVTLQTYIAAMAHQSLKGFTVHQRGMRQALGHMVLNDRTFSQIGP
                     AVNSGRSIFLYGPPGDGKTTIAEAVGAMILNGDISVPYAIEVDGAIIKVFDQSNHERV
                     AKQATSALKNRDLRWVKVHRPVIITGGELTLETLDLVWDEVNNYYQAPYQMKANGGMF
                     LIDDFGRQQVRPRDLLNRWILPLEKRVDYLTLHTGRKIEIPFDVLIVFSTNLAPKDLV
                     DEAFLRRIR"
BASE COUNT          613 a          712 c          694 g          677 t
ORIGIN      
        1 atcgcagtca gcatccgctc agcctctgca ttgcgttcct gccggataag atcgaggcag
       61 cgacggcgca gttcgctggc cgcttcggcc aatccattga ggtacgcggc ttcatccacc
      121 tcgatctcgg tatgggatgg aatagatcga tcgcggacta tagcgtaaac tacgcaagct
      181 tccgtcagtt ctttcaatgc atcctgcgtg tagccggtgt aatacagcgc cggatgctcg
      241 gcgatatcgg ctttcatttc ggcggcaaat tgcttggcac tcgccaatag tttttcggcc
      301 tcgtcaaatt cttcgcgatg ggtcgcccgg atcgtcagcg agcagtagcg gatcagttca
      361 cgcgatcgat tcaaggtgag atcgcgcgcc gtgttgcgtt cggtaaactt cgatcgaatg
      421 gcttcaatga tgctgtccaa tttatccatg gatcaacttc ctgtcggctt cacgccgcca
      481 aacagctgct cggctaatcc cgtgccgccc ggcggcagtt tgatctcgcc gaattgagtt
      541 tcgtacttct gcaaattgtc ggccaaggcg ttcaacatca gcttggcgtg tagcggagtc
      601 atgacaatac gcgtatcgat ctgcgctttg gtcatgcccg gcaacacact cgtgaagttt
      661 aagatcactt cggacggtgt gtgcatgatc aacgcaaagt tagcgtaatg cgctttgagc
      721 tcattcggga tttcaatatt gatcggcgta ggattattcg attgagggag tggatttgat
      781 ggaatgctca tgatcgtcct tgtctggaaa ttggaagttt gaaattagag gcgggcaata
      841 ttttctacac gcaaatacgc gatctaattt ctaatctcca aataaaatga gccgtccacg
      901 aacgaacgca gacggctgtg atctcactgt ccaactcgat agattgccta gaagggaatt
      961 tcttcttcgc caccagccgc tgcaacatct tccggcgcac cactcgggcc ggccgtaccg
     1021 cctgcagcat cgcgcccacc cagaaagcgc acagtgttcg cgaagatttc aaaggatgtt
     1081 ccggtgctgc catcttgccg agtgaagaca cgcggaccgc cagtcgtagg atctggcgtt
     1141 aaacggcctt caaccagcac cgagctgcct ttcttcaaat attgcgcaca cgcctcggct
     1201 tgtttgccaa acaccgacac gcgaaaccag gtcgtttcct tgacttgctg attgtctttg
     1261 tcgttgtatt tgcgattggt cgccacgctg aggctggtga cgggtgtgcc gcttggggta
     1321 tagcgcattt ctggatcgcg tcccaagttg ccaacgagca caatctgctg atacattgtg
     1381 attcctcctg aacaggaaat gggttggacg cccacattga ttcatttgtt cgcagtgaac
     1441 aaacgtacgc aaagtttaca acaagtcgaa tcggttgtca atattttttg catgactttt
     1501 ttcgattggt cgtttcgtac tcgacgcaca catcacgaca aaacgttcta attttgcaca
     1561 aactcgtcga ggccagctaa ccgatcggtt tattactcga gcgaagcgta tggtataatg
     1621 ccgacaatct taatatttcg gtttcactcc gtctcggagg ttgtgtgatg agtcagctcg
     1681 attcaattac cagtccgctc actcctgctt atgaaccgct cgccccgcga tcgattgaag
     1741 aaaccggctt gaacctcggt tttctcaccg aactcgccct caagatcatg tacttcgaag
     1801 gttatctatc gggatcgcag atcgccgatc ggatgaagct gcccttcacg ggtgttgtcg
     1861 atcaagtcct ggagtttctc aagcgcgagc agatggccga agtcaaagga tcgggcggct
     1921 ttggcgagca agcttatcaa tatgccattt cgatgaaagg ccgcgagaaa gctcgcgaag
     1981 cgctcgatcg atcacaatat gccggcgcgg cgccggtcac gctgcaaaca tatatcgccg
     2041 cgatggccca ccaatcgctt aaaggcttca ccgtccatca acgcggtatg cgccaagcct
     2101 tgggtcacat ggtcttgaat gaccggacct tcagccaaat tggtccggcc gtgaattcgg
     2161 gcagaagtat atttttgtat ggtccgcccg gtgacggcaa gaccacgatt gccgaagcgg
     2221 ttggcgccat gattttgaat ggcgacattt ctgtgccgta cgccattgaa gtggacggcg
     2281 cgatcatcaa agtcttcgat caaagcaatc atgaacgcgt cgccaaacag gccacttcag
     2341 cgcttaagaa tcgcgatctg cgctgggtca aagtccatcg accggtgatc atcaccggcg
     2401 gcgaactgac gttggaaaca ctcgatctgg tgtgggatga agtcaacaat tattatcaag
     2461 ccccgtatca gatgaaagcc aacggaggca tgtttttgat cgatgacttt ggccgtcaac
     2521 aagtgcgtcc gcgcgatcta ttaaaccgtt ggattctgcc gctcgaaaag cgcgtggatt
     2581 atttgacgct acacaccggc cgcaaaatcg aaattccatt cgatgtgttg attgtgttct
     2641 cgacgaatct cgccccgaaa gatttggtcg acgaagcctt cttgcgccgc attcgg
//
LOCUS       DASRTJ010000530         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_33700,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000530 DASRTJ010000000
VERSION     DASRTJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33700"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFF70_10770"
     CDS             complement(<1..479)
                     /locus_tag="VFF70_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45221.1"
                     /translation="MFEDRLQKIIAPRLKKFGYVYDLNLREGDELFGFSKNLGDEIAA
                     FVQFQRKGGLSNDEFTVNLLRVKAADLQPRAFGGYASALGARLTHMLWYVHELRDYPA
                     SEYWWTSADRSGLEANLIDAVNQIEKYGLAWIEDPNASRPWEMPEYSAAQFVEVLNQV
                     "
     gene            complement(600..1271)
                     /locus_tag="VFF70_10775"
     CDS             complement(600..1271)
                     /locus_tag="VFF70_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZY45222.1"
                     /translation="MTYFKSVWTIVRKDIITELRSREMLFSMGAFAVLVILLFSFAFD
                     LRVAKAGTVAPGALWSVIAFAGMLGLGRSLAAEIDRGSFDALLLAPIDRSAIFLGKAV
                     GIIIFMSIIEAVILPLFVIFFNLPFLQPQIILVILLGTIGYGGIGTLFSTMAANTRLR
                     EVLLPVLLLPIAIPALLAAVKCTAGALDGRPFEDWASWFGLLVAFDLTMLGAAFLLYD
                     FVVED"
     gene            complement(1415..2110)
                     /gene="ccmA"
                     /locus_tag="VFF70_10780"
     CDS             complement(1415..2110)
                     /gene="ccmA"
                     /locus_tag="VFF70_10780"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZY45223.1"
                     /translation="MITAISLTKQFGPFMALKGIDLNIRPGEFVTIVGPNGAGKTTLL
                     RILATLSRPTGGRVSIAGHNLPKGADDARRHIGLLSHQPLLYGDLTAEENLRFFGRLY
                     NVSNLEKRIFDLLDEVDLLHRRADRARTFSRGMQQRLAIARALMHDPDVVLLDEPFTG
                     LDPDASDRLEGLLRGLHNGSRAVVMTIHDLDRGWAMCNRALVIARGKIVFEASTQEVD
                     QATFRSEYHRVTR"
     gene            complement(2185..>2967)
                     /locus_tag="VFF70_10785"
     CDS             complement(2185..>2967)
                     /locus_tag="VFF70_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45224.1"
                     /translation="TEDTSQVSIDTLHIVAIPNATGLQVSEIYVLSNSGDRFVAGFGK
                     PMMHLSLPNGAASISADPNMQPDTLIPNGDGVDYYDAVPVGIGTAQIIFQYQLSGSNF
                     VLDRPVNQKVSSINLLVQGDSKQLEVASTQFLNQGTQSLPGQNQVFQQYTAQNLKPGD
                     KLSIAISMPGASIDWRVLLGGVLIAVGVIGVIAWQVQQRRNAKSTGRRPMAVKIEDDQ
                     DSLLDQIAALDDVHKAGQIDEASYNTKRAQLKKKLMQLMSNE"
BASE COUNT          734 a          843 c          741 g          649 t
ORIGIN      
        1 acttgattca acacttcgac aaattgagcc gccgagtatt ccggcatctc ccacggtcga
       61 gatgcgttgg gatcttcaat ccaggcgagg ccatactttt caatttgatt gactgcatcg
      121 atcagatttg cttcaagacc cgatcgatca gcggatgtcc accaatactc ggacgcagga
      181 tagtcgcgga gttcatgcac ataccacagc atatgcgtca gccttgcgcc caacgcgctc
      241 gcgtaaccgc caaatgcgcg cggctgaagg tcggcggctt ttacacgcaa gaggttcacg
      301 gtgaactcat cgttcgataa gccgcccttt ctttgaaact gcacgaacgc ggcgatctcg
      361 tctccaagat ttttgctaaa cccaaaaagt tcgtcgcctt cgcgaagatt cagatcgtag
      421 acgtagccga attttttcag gcgcggtgca atgattttct ggaggcgatc ttcgaacata
      481 cgattatcgt aggggcgagg catttgcaca tcaatccttg aagcacgcgg tgatggacac
      541 ttcagcaaag cttaacaaca tcgatctcat tacaaatgcc tcgcccgtac ccaagaaaat
      601 caatcttcca ccacgaaatc atacaacaaa aacgctgcgc ccaacatcgt cagatcgaac
      661 gcgaccagca gcccaaacca actggcccaa tcttcaaatg gccggccatc gagcgcgcca
      721 gcggtgcatt tcaccgcggc caataacgcc ggaatcgcga tcggcagcag caggactggg
      781 agcagcactt cgcgcaagcg cgtattagcc gccatcgtcg agaacaacgt gccgatgccg
      841 ccatacccga tcgtgcccag cagaataacc aaaataatct gcggctgcaa gaacggcaga
      901 ttgaagaaga tgacgaacaa gggcaggatc accgcttcga tgatcgacat gaagatgatg
      961 atgcccaccg ctttgcccag aaagattgca ctgcgatcga tcggcgcgag cagcagggca
     1021 tcaaaactgc ctcgatcaat ttcggcggcc aacgatcgac ccagccccag catgccggcg
     1081 aaagcgatca ctgaccacaa cgcgcccggc gcaaccgtgc ccgctttggc cacgcgcaga
     1141 tcgaacgcga acgagaacag cagaatgacc agcacggcaa acgcgcccat actgaagagc
     1201 atctcgcggc tgcgcagttc ggtgatgata tcttttcgga caatcgtcca aacacttttg
     1261 aagtaggtca tgattatcga tcatcattgc gaggcgcact ctgtccgccg acgcctggca
     1321 gaccaactga gtactttgta ctcagggtat gggcagcaat ctcagtacac aattaacgag
     1381 attgcttcgt cgcttcgctc ctcgcaatga cgtatcacct tgttacacga tgatattccg
     1441 atcggaatgt ggcttgatca acttcttgag tactggcttc gaaaacaatt ttgccgcgcg
     1501 cgatcaccaa cgcccgattg cacatcgccc agcctcgatc gagatcgtgg atcgtcatca
     1561 ccaccgcgcg cgatccgtta tgcaggccgc gcagtagtcc ttccaatcga tcggaggcgt
     1621 ccggatcaag tccggtgaac ggttcatcga gcagcacgac atccggatca tgcatcaagg
     1681 cacgcgcgat cgctaaccgc tgctgcatcc cgcgcgaaaa cgtccgcgca cgatcggccc
     1741 ggcgatgaag cagatcgact tcgtcgagca gatcgaaaat ccgtttttcg agattcgaca
     1801 cgttatacaa tcggccgaag aaacgcagat tctcttcggc cgtcagatcg ccatagagca
     1861 gcggttgatg cgacaacaaa ccgatgtgac gacgcgcatc gtctgctcct ttcggcagat
     1921 tgtgtcccgc gatcgaaacg cggccgcccg tcggtcggct caatgtcgcc aggatgcgca
     1981 gcagcgtcgt cttacctgca ccattcggcc cgacgatcgt cacgaattcg ccgggccgga
     2041 tgtttaggtc gatacctttt agcgccataa acgggccgaa ttgtttggtg agggagattg
     2101 cggtgatcaa gtgatactca aagcattcaa aaattgattt cgaattccta cctgcagatt
     2161 agtagatagg caatcgtgtt gcgcttattc gttactcatc agctgcatta gcttcttctt
     2221 caactgcgcg cgtttggtgt tgtaactcgc ctcatcaatc tgcccggcct tatgcacatc
     2281 atcgagcgcg gcgatttgat cgagcagaga gtcttgatcg tcttcgatct taacagccat
     2341 tggccgccgg ccagtagatt tggcattgcg cctctgctga acttgccacg caattacgcc
     2401 aatgacgccg actgcaatca gcacgccgcc gagcagcaca cgccaatcga ttgatgcgcc
     2461 gggcatagaa atagcgatcg agagtttatc acctggcttt aagttctggg ctgtatattg
     2521 ctgaaaaact tggttctgcc ctgggagcga ttgggtgcct tgattcaaga attgagtgct
     2581 ggccacttcc aattgtttcg aatcaccctg aaccaataag ttaatcgaac tcactttttg
     2641 gtttaccggc cgatcgagca cgaagttaga cccagacagt tgatattgga aaataatctg
     2701 agccgtgcca attccgaccg gcaccgcatc gtaataatcg acgccgtcac cattcggaat
     2761 caacgtatcc ggctgcatgt tgggatcagc ggagatcgag gccgcaccat tcggcaaact
     2821 gagatgcatc attggtttgc cgaagcccgc tacaaagcga tcgcccgaat tggacaggac
     2881 atagatctcg ctgacttgca gaccagtcgc attcggaatt gccacaatgt gaagtgtatc
     2941 gatgctcact tggctagtgt cttcagt
//
LOCUS       DASRTJ010000531         7256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_71854,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000531 DASRTJ010000000
VERSION     DASRTJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71854"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VFF70_10790"
     CDS             complement(<1..1058)
                     /locus_tag="VFF70_10790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45225.1"
                     /translation="MIAPLNDETEPEVRPGAHPLRALRTRNFKLFFFGQFISLIGTWM
                     QNTALPLLVIQLAPDHPGEALGLVNFVPLIPLVPLVLIVGSLADRFSRRKLVILAQIV
                     MMLTAFTLAYLTLTQQIQIWHIFALIMANGIGNAIDTPSRQAFMVDMLEDRSDLGSAV
                     ALNSSIFNLGRALGPVIAGFLIAPLGFGAAFLINGLSFLAVIIGLLLIHIPPQPPRDR
                     QPNLGTHLSEGLRYVWQNQSVLILMSLVAVSAFLSTPFLTLMPLFVQAPNGSLAATAQ
                     PINDLICSRIVCQTADAIPFGLLMGAFGLGALVGALLVGAKTDRLRGRWLTLGNLGFP
                     MALIVFAVSRSFWLSFAV"
     gene            complement(1061..3292)
                     /locus_tag="VFF70_10795"
     CDS             complement(1061..3292)
                     /locus_tag="VFF70_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY45226.1"
                     /translation="MTIRSHAVIIFEANLPDRPKMIATNSPSTSSNPIRYYAISLLFP
                     LSILLALRAIPQIDLNLNSPTAHFYIVTAVSFIGIVLAGQAHTLARVSGRAQILFVSL
                     GMFSVTGLLFIHGLATAGALLPEPTPGAGWSARLSLVVGGIFFVLSGTRWPASISLVL
                     VKRYRSIIAAAWIIYAAFFLVVFSFPAQLAGLDKTGSTGVLITLIAAITLYSVALILS
                     LNRSRVEHKRLDAFITIGLLLFGAAVASQSVGILWHLSWWSYHFLIGLAIIVMAVGLN
                     HEYGHLRPANLQRTYFLFGLTVIALLALVASEIFFRINRDNLTHERETSAIVLAQHVA
                     IELQSELPQITDPVGLQSLASIPQVKEIIDHNLVGLNIPRVKIYDAIGQIVYSTASFT
                     LGQAQANDALLSAITGQPRSLLEDNTEPEVPGEPALAGQIIETYVPLRNANQEIIGAF
                     EIYQDANDLIATAILQRGQFLITVSILMLLLFGGFVLIVRRADQLVTARTAELAATRF
                     SLQQSERTRQDMTNVVISNVRRPMATLTRSIDQIQRGTLSPEQGELIGRARNNAREVN
                     NTIYNLLNISQIENGELHLQQSTFPINDLLQVTVQQVAESARDAEVTVRSEVRPGLPY
                     VWADRDLIGRVINNLLSNAIKHTRRGGRLLLNADVEPGGNIVVRVQDQGAGITVEDQA
                     HLFDKFARIGGEATGDSTGLGLAFCKMTIEQHQGRLWVESTVGQGSTFAFTLPTLTTA
                     AAI"
     gene            complement(3345..3417)
                     /locus_tag="VFF70_10800"
     tRNA            complement(3345..3417)
                     /locus_tag="VFF70_10800"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3382..3384),aa:Asn,seq:gtt)
     gene            complement(3493..5091)
                     /gene="rpoD"
                     /locus_tag="VFF70_10805"
     CDS             complement(3493..5091)
                     /gene="rpoD"
                     /locus_tag="VFF70_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZY45227.1"
                     /translation="MRQSKAELLPLREDELNNDDELLDEELDLEEPGLDTPGGSIEDD
                     ESLLADEPDIAELEEIETEEEEESDLEVGADLADDPVRMYLKEIGQVRLLDSQQEIWL
                     AIQMSAAGQLDRVRDRLHAANKKSPQPAELMLGVYADFVAAWKQLEKLGRKVHLKKIS
                     LPGLTQETRLLRIGAPMTTRSLLVVQSGGRNPEHRSSALSAALGQLYSAYVALYLLPI
                     ETLDWLIVQTTKNKTKLPTPKAVTKSIPEARSIKQSIQETLDSSERARQSLTRANLRL
                     VVSVAKRYIGRGISFLDLIQEGNIGLLRAVEKFDYTKGYKFSTYATWWIRQAISRAIA
                     DQARTIRIPVHMVETINRLVRIQRKLVQELGREPSSEEVAVEMGLLEPDETAAVQLAW
                     ATNQPLDPAMERTLRRGAAKVRRIVRISQEPMSLETPVGQEESSLLGDFIEDESVLGP
                     VDAASSQLLKEQVHSILDSLSEREREVLEMRFGLSDGQGHTLEEVGKFFGVTRERIRQ
                     IEAKALRKLRHPYRSRKLRDYLAE"
     gene            complement(5233..7158)
                     /gene="dnaG"
                     /locus_tag="VFF70_10810"
     CDS             complement(5233..7158)
                     /gene="dnaG"
                     /locus_tag="VFF70_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZY45228.1"
                     /translation="MTVIDDIKDRIDIIDIVSETVKLKKSGRTYTGFCPFHANSHTPS
                     FVVWPETGTWKCFGACNTGGDVITYVMKRDGLEFKDALHALGRKAGIEVVDRKPDAEI
                     EDQHIARLREAVAAAAQWYNFILSNNPQAQAARDHLSKRGLTAKTTEVFQLGYAPDDW
                     HAFENQLRDKGFTREEMIDAGLLVSRDDGNVFDRFRNRLMIPIHDGKGRPIGFGARAL
                     KEGDEPKYLNSPQSSLFDKSRTLYGLHAARTAIRDQKVAVIVEGYMDALAAHQFGFAN
                     VVASLGTALTELQFKQLQKLTKRIVLALDPDTAGINAMLRGLDVARESLDRELRPVFD
                     ARGLIGNASKLDIDLRVLTLPGGQDPDEVMQHDPTQWNTLVDQAPPVAQFVIDSLTAG
                     RNLNDPKEKAAIANQVLPIIRDIANPVEQNVYIQQLARKLKVDERSMFDQMRVAVASA
                     ARSRSRTAAQSIEPIKHDAADVEQYCLSTLLHRSDMLKVIDDALSRAELPALNIEDFA
                     EAANREVFKAFQASLSRSPIIDDESLRSELDPTLHDHLSALINEAIKAPSGSTSNAID
                     QDAQQVGLRLRERRLRREGQELHALLEDSVNDQVDDAQALYQARQLNATALLRLQQIL
                     SARTITRQSTTEPWSRS"
BASE COUNT         1618 a         1957 c         2061 g         1620 t
ORIGIN      
        1 aagaccgcga aggacagcca gaacgatcga gaaacggcaa agacgatcag tgccatcggg
       61 aaaccgaggt tgcccagcgt caaccatcgg ccgcgcaatc gatcggtctt tgccccgacg
      121 agcaatgcgc ccactaatgc gcccaggccg aacgcgccca ttaacaaacc gaatggaatc
      181 gcatcggccg tctgacagac aatgcggctg cagatgagat cgttgattgg ttgggcggtg
      241 gcggcaagtg agccattcgg cgcttggacg aagagcggca tcaacgtcag gaatggtgtc
      301 gagagaaagg ccgagacggc gacgaggctc attaagatca acacgctttg attctgccag
      361 acgtagcgca ggccttcact cagatgcgtg ccgagattgg gctgtcgatc gcgcggcggc
      421 tgcggcggga tgtggatcaa cagcaggccg atgatcacgg cgaggaacga taggccgttg
      481 atcagaaaag ctgcgccgaa accgagcggg gcgatcaaga atccagcgat aacgggtccg
      541 agggcgcggc ccaagttgaa gatcgatgaa ttgagcgcga ccgcgctacc gagatcgctt
      601 cgatcttcga gcatatcgac catgaaagcc tggcgtgacg gcgtatcgat cgcattgccg
      661 atgccgttgg ccatgatcaa ggcgaagatg tgccagatct ggatttgctg tgtcaaggtg
      721 agataagcca gcgtaaaagc cgtcagcatc ataacgattt gcgcgaggat gaccaacttg
      781 cggcgggaga atcgatcggc taatgaaccg acgattaaca cgagcggcac gagcggtatc
      841 agcggaacaa agttgaccag ccccaacgcc tcaccgggat gatcgggcgc aagttgaatg
      901 accagaaggg gcagtgccgt gttctgcatc caggtgccga tcagtgagat gaattgtccg
      961 aaaaaaaata atttgaagtt gcgagtgcgc agcgcgcgca ggggatgagc accgggacgg
     1021 acttccggct cggtctcgtc atttagcggt gcgatcattt ttagatggca gcggccgtgg
     1081 tgagtgtggg caaggtgaaa gcgaaggtgc tgccttggcc gacggtgctt tcgacccaca
     1141 ggcggccttg atgctgttcg atcgtcattt tgcagaaggc taaacctaaa ccggtgctat
     1201 caccggtcgc ttcgccgccg atgcgggcaa atttgtcgaa gaggtgggct tgatcttcga
     1261 ccgtgatgcc agcgccttga tcttgtacac gcacaacgat gttgccgccc ggctcgacat
     1321 cggcgttgag cagtaatcgc ccgccgcggc gcgtatgctt aatggcgttg ctgagcaggt
     1381 tgttgattac acggccgatc aaatctcgat cagcccagac gtacggtaag ccgggccgca
     1441 cttcagatcg cacggtgact tcggcgtcgc gcgccgattc ggcgacttgt tgtacggtga
     1501 cctggagcag atcgttgatc gggaaagtgg attgctgcaa atgcaattca ccattctcga
     1561 tctggctgat gttcaataaa ttgtaaatag tgttgttgac ttcgcgcgca ttgttgcgcg
     1621 cccggccaat gagttcacct tgttcggggc tgagggtgcc gcgctgaatt tgatcgatcg
     1681 agcgggtgag cgtggccatg ggccgccgca cattgctgat gacgacgttg gtcatatctt
     1741 ggcgtgtgcg ctcggattgt tgcagcgaga atcgcgtggc agccaattca gctgtgcggg
     1801 cggtgaccag ctgatcggcg cggcgaacga tcagaacgaa tccgccaaaa agcagtagca
     1861 tcaagatcga tacggtgatc aagaattggc cgcgctgcag gatagctgtt gcaatgagat
     1921 cgttggcatc ctgataaatt tcgaacgcgc cgataatttc ctggttcgca ttgcgcagcg
     1981 gcacgtaggt ctcaatgatt tgaccggcga gtgcgggttc acctgggact tccggctcgg
     2041 tgttgtcttc caacagggag cgcggctgac cggtgatcgc gctgagcagc gcgtcgttcg
     2101 cctgtgcttg gccgagggta aagctcgcgg tgctgtacac gatctggccg attgcatcat
     2161 aaatcttgac gcgtgggatg ttaagtccga ccaaattgtg atcgataatc tctttgactt
     2221 gtgggatgga ggcaagagat tgcaatccga ccggatcagt aatttggggc aactcgcttt
     2281 gcagctcgat cgctacgtgc tgcgcgagaa caatggccga agtttcgcgc tcatgggtca
     2341 gattgtcgcg attgatacga aagaaaattt cactcgcgac taaggccagc agagcgatca
     2401 cggtcaggcc gaacaaaaaa taagtccgct gcagattagc cgggcgcaga tggccgtact
     2461 cgtgattcaa gccaactgcc ataacgatga tcgccagacc gatcaagaag tgatacgacc
     2521 accacgagag gtgccagaga attccgacgc tttgtgaagc gaccgcagct ccgaaaagca
     2581 gaagaccgat cgtgatgaag gcatctaatc gcttgtgttc cactcgcgat cggttcagag
     2641 acaagatcaa ggcgacagag tataacgtta tcgcggcaat caaagtaatc agcacaccgg
     2701 tcgatccggt tttatcaagc ccagccagtt gggccgggaa tgagaaaacg acgaggaaga
     2761 aagctgcata gataatccaa gccgcagcga tgatggatcg ataacgcttg accaaaacta
     2821 ggctaatcga cgcaggccag cgggtaccgc tcagaacgaa aaagattcct ccgaccacca
     2881 gactgagtct ggccgaccag ccggcccccg gcgttggttc gggcagcaat gcgcctgcgg
     2941 tcgccaagcc gtgaatgaag agcaagcccg tcacggagaa catgcccagc gacacgaaca
     3001 ggatttgtgc gcggcctgac acgcgcgcga gcgtgtgagc ttgaccggca aggacgatgc
     3061 ctataaagct aactgcagtg acaatgtaaa aatgtgctgt tggactgttg aggtttaaat
     3121 cgatctgcgg aattgcgcgc agcgccagca gaatactcag cgggaacagc aagctaatgg
     3181 cataatagcg aattggattg cttgaggttg aggggctgtt ggtggcgatc atcttgggcc
     3241 gatcgggaag gttagcctca aaaattataa ccgcgtgaga gcgaatggtc aaacgacgca
     3301 gcgaacaaaa aaagagacgg caggccgtct ctttttattt caggctcctc gaccaggact
     3361 cgaacctgga accgatcggt taacagccga ttgctctgcc gattgagcta tcgaggaatc
     3421 gttggctgat tataccagtg cgctccagga gcgtcaagaa aaaaattata aagacgttcc
     3481 agcggaacgt ctttactctg caagataatc tctgagcttg cggctgcgat agggatgccg
     3541 cagcttgcgc agcgccttcg cttcgatctg gcggatgcgt tcgcgcgtga cgccgaagaa
     3601 ctttcctact tcctcaagcg tatggccttg tccatcactc agcccgaaac gcatctctaa
     3661 aacttcgcgc tcgcgctcgg acagcgaatc gaggatcgaa tggacttgtt ctttcagcag
     3721 ttgggaggag gccgcatcga ccgggcccag taccgattca tcctcgatga aatcgccaag
     3781 cagcgatgat tcttcctgcc cgaccggcgt ttcgagcgac atcggctcct ggctgatacg
     3841 gacgatgcgc cgcactttgg ccgcgccgcg ccgcagtgtg cgctccatcg ccggatcgag
     3901 cggttgattc gtggcccacg ccaattggac cgccgctgtt tcatcgggtt caagcaaacc
     3961 catttccacc gcgacttctt cggaagaggg ttcgcgtccc aactcttgca ccaacttgcg
     4021 ctgaatgcgc accaagcgat tgatcgtttc gaccatatgc accggaatgc gaatggtgcg
     4081 cgcttgatcg gcaatcgcgc ggctgatcgc ttgtcgaatc caccaggtgg catacgtgct
     4141 gaacttgtaa cctttggtgt aatcgaattt ttctaccgcg cgtagcaaac cgatattgcc
     4201 ctcttgtatc aaatctagaa acgatatacc gcgcccgatg taacgtttgg cgacgctgac
     4261 gaccaggcgc aagttggcgc gggttaatga ttgccgcgcg cgttccgatg aatccaatgt
     4321 ttcttgaatc gattgcttga tcgatcgtgc ttccgggatc gattttgtga cggccttcgg
     4381 tgttggcagc ttagttttgt tcttcgtcgt ctgcacgatc agccaatcga gcgtttcgat
     4441 cggcagcaga tacaaggcca cataggcgct gtacagctgg ccgagtgcgg cgcttaatgc
     4501 actgctgcga tgctccggat tgcgtccgcc ggattgcact accagcagag agcgcgttgt
     4561 catcggcgcg ccgatgcgca gtagacgcgt ttcttgcgtc aagcccggca ggctgatctt
     4621 tttcaagtgg actttgcgtc ctaatttttc caactgcttc caggcggcga cgaaatcagc
     4681 atagacgccc agcatgagtt cagcgggctg gggcgatttt ttgttcgccg catgcagtcg
     4741 atcgcgaacc cgatcgagtt gcccggccgc gctcatctga atagccagcc agatttcttg
     4801 ctgcgaatcc agtaggcgca cctggccgat ctctttgaga tacatgcgca ctggatcgtc
     4861 ggctagatca gcaccgacct cgagatccga ttcttcttcc tcttcggtct caatctcttc
     4921 cagctccgca atgtccggct cgtcggctaa taggctctcg tcgtcctcga tcgatccacc
     4981 gggcgtgtca agacccggct cttccagatc gagctcttcg tcaagcagct cgtcgtcgtt
     5041 attcaattcg tcttcgcgta aaggtagcaa ttcagccttc gattggcgca tcgacccgtg
     5101 tcaagcgatc gcgcaaagct ttagattttt ttgaccccga tcggctaagg gcgccgatgg
     5161 aatgatattg gagcagcaat tgtactgccg cgacgtcatc cgcctgccgc ttgaaagttt
     5221 gatcccgatc agctaactgc ggctccacgg ttccgtcgtg ctttggcgcg ttattgtacg
     5281 cgcagacaag atttgttgca accgcaataa agccgtcgca ttcaattgcc gagcttgata
     5341 caacgcttgg gcgtcatcca cttgatcgtt cacgctatct tcgagcagcg cgtgcagctc
     5401 ttggccttcg cgccgcaagc gccgttcacg caaccgcaag cccacttgct gcgcatcttg
     5461 atcgatcgca ttgctggttg atccacttgg cgctttgatc gcttcgttga tcaacgccga
     5521 caaatgatcg tgcagcgtcg gatcaagctc tgagcgtaaa ctctcatcat caatgatcgg
     5581 tgagcgactt aagctggctt gaaatgcttt gaacacttcg cgattagcgg cctcggcaaa
     5641 atcctcgatg ttcaacgcgg gcagttcggc gcggctgagc gcgtcgtcga tgactttcag
     5701 catatctgat cgatgcagca gcgtcgataa acaatactgt tccacatcgg ccgcatcgtg
     5761 cttaatcggc tcgatcgatt gggcggcagt gcgtgatctc gatcgagccg ccgaggccac
     5821 cgctacacgc atttgatcga acatggatcg ctcatcgact ttcaatttgc gcgccagctg
     5881 ctgaatgtaa acattttgct caaccggatt ggcaatgtcg cggatgatcg gcagcacttg
     5941 attggcgatg gccgctttct ctttgggatc gttcagattc ctgcccgctg tcaatgaatc
     6001 gatcacaaac tgtgccacgg gcggcgcttg atcgaccaac gtattccatt gagtcggatc
     6061 gtgctgcatc acttcatcgg ggtcttgacc cccgggcaac gtgagtacgc gcagatcaat
     6121 atccaacttg ctcgcgtttc cgatcagacc gcgcgcgtcg aagaccggcc gcagctcacg
     6181 atcgagcgac tcgcgggcca cgtcgaggcc gcgcagcatc gcattgatac ccgccgtatc
     6241 cggatcgagt gccagcacga tgcgcttggt tagtttctgc aattgcttga actgcagctc
     6301 ggtcaaggcg gtgcctagcg aagcgacgac attcgcgaaa ccaaattgat gcgccgcgag
     6361 tgcatccata taaccttcga cgataacggc cactttctga tcgcggattg cagtgcgcgc
     6421 ggcatgcaag ccgtacaacg tgcggctctt atcgaacaat gagctttgcg gtgaattcaa
     6481 atacttcggt tcgtcaccct ctttaagcgc gcgtgctccg aatcctatcg ggcggccttt
     6541 accgtcgtgg atcgggatca tcaatcggtt gcggaatcga tcgaagacgt tgccatcgtc
     6601 acggctgacc agcaaacccg catcgatcat ttcttcgcgg gtaaagcctt tatcgcgcaa
     6661 ctgattttca aaggcatgcc aatcgtctgg tgcataaccc agttgaaaaa cctcggtcgt
     6721 cttggcggtg aggccgcgtt tcgataaatg atcgcgcgcg gcctgtgctt gaggattgtt
     6781 gctcaagatg aagttgtacc actgcgccgc ggcggcaact gcttcacgca atcgcgcgat
     6841 gtgttgatct tcgatctcgg catcgggctt gcggtccaca acctcaatgc ctgctttgcg
     6901 gcccaaagcg tgcagcgcat ctttaaattc aaggccgtcg cgtttcatca cgtacgtgat
     6961 cacatcgccg ccagtgttgc acgcgccgaa gcatttccag gtgccggtct ccggccacac
     7021 gacaaacgat ggcgtatggc tgttagcatg aaacggacag aacccggtgt aggtccggcc
     7081 gctcttcttc agcttcaccg tctcggatac gatgtcgatg atgtcgatgc gatctttgat
     7141 gtcatcaata acggtcatgg gaaatttaca atgaacaatt aacaatgacc aatgacaaat
     7201 gatcgataat cgtaattagc tgcctagaag ttacttcttc cattttgctt tgctgc
//
LOCUS       DASRTJ010000532         4611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_279523,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000532 DASRTJ010000000
VERSION     DASRTJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279523"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFF70_10815"
     CDS             <1..1185
                     /locus_tag="VFF70_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45229.1"
                     /translation="AYISIGYSTNGNLVSSAKDANPAVSDTPRWTTLSWNATTPTSTT
                     LKFQAAASNSSTGPYTFVGPDGTANTYFTSGGSLSQFDGLRYLKYTAYFTTTDSTATP
                     TLADVTVCFNNIAISPTVWNGNTDTNWFVAGNWNPASVPTAASEVIIPTAPSGNRWPT
                     LTGTTTIYTLTLQSSAALTLAQGITLSVNGAVTNDGALAQVNDVPASAMTEFLHITDS
                     SGTIDKYHGVDLTPGGTAMGVTTVQVKGNQSACTTVPADPIIQRCYRIDPTTQTTATV
                     RFWFTEAERNAQTANALKLWHWGPWTQVGTAGNYTYSESGVACLSGSGLACWFQSTGV
                     ASYSPFAVGSTTAPTAITLRGFSAISSSSSPVFLIAVILAAATAIGAVAVSRKKRRIT
                     LQ"
     gene            1297..2067
                     /locus_tag="VFF70_10820"
     CDS             1297..2067
                     /locus_tag="VFF70_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZY45230.1"
                     /translation="MFTRAIVRPPEHNFADGLTTVDLGMPDFQKALQQHERYCLALEE
                     CGLTLMRLAADVRYPDSTFVEDTAILTERGAILTHPGALSREGEMISITETLSKFYAE
                     FQTITEPGTLDGGDICEAGQHFFIGISHRTNEAGAQQLAEFLAAQSYTSSCVDIRNID
                     GILHLKSGIAYLGENRLVLIDALADRSEFRGHSIVHVAPEENYAANCVRVNDNVLIAQ
                     GYPILEKTLSNLGYKTIVLNMSEYQKMDGGLSCLSLRF"
     gene            2202..4436
                     /locus_tag="VFF70_10825"
     CDS             2202..4436
                     /locus_tag="VFF70_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZY45231.1"
                     /translation="MDSLEGIVERITYYNEENGYTVLRLSAPGYAELVTVIGNLPEIT
                     PGESLRLRGEWATHQKHGKQFKADQCEQILPASIEGLKKYLGSGLIKGVGPVTAARIV
                     KKFGAETLDTLDRAPQRIREVLGIGPKRADLITRAWDEQKSIKQVMVFLQGHGVTTGL
                     AVKIFKQYGDASIDLVQADPYRLATDIYGIGFKTADKIAHDLGLPHDAPSRVAAGVVY
                     TLSNFADQGNVFAPQAQLVHEAAELLDVQPDQVRSSLDTLEKSDQIKREIIYPIVGSR
                     QKDTSALIPLPSSLKEDAAIYLTPFYHGEVGVAKRLNAIIDARQSRLSELARSDLQKI
                     LNDLGSTVASHSTARLSDQQRDAIKSAVTHKVTVLTGGPGTGKTTAMRALIELLEKFH
                     KTYALSSPTGRAAKRLSQATDRSAKTIHRLLEFSPNEGFKRNEKNPLDIDILIVDEAS
                     MIDLLLMNHLLKAVPIDAHLLLVGDIDQLPSVGAGDVLRDIIDSGRAAVIRLDVIFRQ
                     AQNSHIITNSHRINQGQMPLTPAEADDFFLFVKEEPDEAADLIVDLVATRIPAKFGLK
                     INDLQVLSPMYRGSTGVSALNAKLQEKLNPPSAKKIERRLGGTLFRVGDRVLQTRNNY
                     DKDVYNGDMGYVTAIDLENQTLTVSIDGRPIEYDWSEADEVVLAYAISVHKAQGSEYP
                     AIVMPLLTQHYMLLQRNLLYTAITRAKKLVVLVGSRRALALAVKNNKPSERFSGLKER
                     LSSV"
     gene            complement(4422..4583)
                     /locus_tag="VFF70_10830"
     CDS             complement(4422..4583)
                     /locus_tag="VFF70_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45232.1"
                     /translation="MEELFRRGFTQDLTPDLAVGQMLQVIQGLWRRIEDLEVRNRLEG
                     TIGSPLHRR"
BASE COUNT         1096 a         1345 c         1212 g          958 t
ORIGIN      
        1 gcctatatat cgatcggcta ctccaccaac ggaaatctgg tctccagtgc aaaggatgcg
       61 aatccggcgg taagtgatac gccacgctgg acgacgctat cgtggaacgc aaccacgccg
      121 accagcacta cgctcaagtt ccaggccgcg gccagcaaca gctcaacagg tccgtacact
      181 ttcgtcggac cggatggcac ggcgaacacc tacttcacca gcggtggatc actcagccag
      241 ttcgacggtc tgcgctatct aaagtatacg gcctatttca ctaccacgga cagcaccgcc
      301 acgccgacac tggccgatgt gacagtgtgc ttcaacaaca tagccattag cccaacagta
      361 tggaacggca ataccgacac gaactggttc gttgcaggca actggaatcc cgcttccgta
      421 ccgacggcag cgtcagaagt gatcattcca accgcgccta gtggtaatcg atggccgaca
      481 ctcaccggca cgaccactat ctacacgctt acgcttcaaa gcagcgccgc gctaactctg
      541 gcgcaaggta tcacgttaag tgtgaacggc gcggtgacca acgatggcgc actggcgcaa
      601 gtcaatgatg tgccggccag cgcgatgact gaattcctgc acatcaccga ttcctccggc
      661 accatcgaca aatatcacgg tgtcgatctc acgccgggcg gcaccgccat gggcgtgacg
      721 accgttcagg tcaaagggaa ccaatcggct tgcacgacgg ttcccgccga cccgatcatc
      781 cagcgctgtt accggatcga tcccacgaca cagacgacgg ccacggtgcg cttctggttc
      841 accgaagccg aacgcaatgc acagaccgcc aatgccttga agttgtggca ttggggtcca
      901 tggacgcagg ttggcacagc cgggaactat acctactcgg aaagcggagt agcctgcctg
      961 tcgggcagcg ggctagcctg ctggttccag tcgacgggcg tcgccagtta ctcgccgttt
     1021 gcggtgggca gcaccaccgc gccaaccgcc atcaccctgc gtggcttctc tgctatttca
     1081 agttcgagca gccccgtgtt cttgatcgca gtgatactgg cggcagccac agccattggc
     1141 gcagtggctg tgtcaagaaa gaagcgaagg attacgcttc aataagttag actctgcttg
     1201 cgaggcggct gtaaaaatca gccgcctcgt ttcattttgc cgcattgatc agtctatgct
     1261 atcatcgcaa tcacctcgcg tcagaggagc aatcgcatgt tcacacgcgc catcgttcgg
     1321 ccgccggaac ataactttgc cgacggcctc accactgttg atctgggaat gcctgatttt
     1381 cagaaagccc tgcaacagca tgaacgctat tgcctcgcac tcgaagaatg cggactcacc
     1441 ttaatgcgct tggccgccga tgtgcgttat cccgattcga ccttcgttga agacactgcg
     1501 attctcaccg aacgcggcgc gatcctgaca catcccggcg cactcagccg cgagggcgaa
     1561 atgatcagca tcacagaaac gttgagcaaa ttctatgcgg aatttcaaac catcaccgaa
     1621 cccggcacac tcgatggtgg cgatatttgc gaagcgggcc agcacttctt catcggtatc
     1681 tcgcatcgaa caaatgaagc aggcgcgcag cagttagcgg agtttctagc ggctcagagt
     1741 tacacctcta gttgcgtgga cattcggaat atcgatggca ttctgcacct caaaagtggt
     1801 atcgcttatc ttggcgaaaa ccgattagtc ttgatcgatg cgctggccga tcgatcggag
     1861 tttcgcggtc actcgatcgt gcatgtcgcg cccgaagaga attatgccgc caactgcgta
     1921 cgtgtcaacg acaacgtgct gatcgcacaa ggttatccga ttttagagaa gacactcagc
     1981 aacttgggtt acaaaacgat cgtactgaat atgtccgaat atcaaaagat ggacggcggc
     2041 ttaagctgtc tgtcactacg attttagtcg tttagttttg gagtctcacc gatccgctga
     2101 tttgccttct ctctttcact cgttcctcat acctgcactg caccgcacgg cgtgcggtgc
     2161 aagtgtcact cgtcactccg tcatgtgtta tcatcaccgc catggattcc ctcgaaggca
     2221 ttgtcgaacg gatcacttac tataatgaag agaacggcta taccgtgctg cgcctgagcg
     2281 cgccgggtta cgccgagctg gtcaccgtca tcggcaatct gccggaaatc accccgggcg
     2341 aatcgctgcg cctgcgcggt gaatgggcca cgcatcaaaa gcacggcaag caattcaaag
     2401 ccgatcagtg cgaacaaatc ctgcctgcca gtattgaagg cctcaaaaag tatttaggct
     2461 ccggcttgat caaaggcgtc ggcccggtca cggcggcgcg catcgtcaaa aaatttggcg
     2521 cggaaacttt agacacgctc gatcgagcgc cgcagcgcat tcgcgaagtg ctgggcatcg
     2581 gtccaaaacg cgcggacttg atcacacgcg cgtgggacga acagaagtcg atcaagcaag
     2641 tcatggtctt cttgcaaggc cacggcgtca ccaccggcct cgccgtcaaa atattcaagc
     2701 aatatggcga cgcgtcgatc gatctggtgc aggccgatcc gtatcggctc gcgactgaca
     2761 tctacggcat cggcttcaag accgccgata agatcgcgca cgatctcggc ctgccgcacg
     2821 atgcgccctc gcgcgtagcc gcaggcgtgg tgtacacgct cagcaacttt gccgatcaag
     2881 gcaatgtttt cgcgccgcaa gctcaactcg ttcacgaagc ggctgaatta ctggacgttc
     2941 agcccgatca agttagatca tcgctagata ctttagaaaa aagcgatcag atcaagcgcg
     3001 aaatcattta ccccatcgtt ggatcacgcc aaaaggatac ttcagccctc atccctctgc
     3061 cctcatccct caaagaagat gccgcaattt atctcacgcc cttctatcac ggcgaggtcg
     3121 gcgtcgctaa acgcctgaac gcgatcatcg atgcgcggca atcccgattg agcgagctgg
     3181 cgcgatcgga tttgcagaag atcttaaacg atctcggatc gaccgtcgcg agtcattcga
     3241 ccgcacggct gagcgatcag cagcgggatg cgatcaaatc ggccgtcacg cataaagtaa
     3301 ctgtcttgac cggcggaccc ggcacgggca aaacgacagc aatgcgcgca ctgatcgaat
     3361 tgctggaaaa attccacaaa acgtacgcgc tctcgtcgcc gacaggccgc gccgcgaaac
     3421 gactgagcca agccaccgat cgatcagcca agaccatcca tcggttgttg gagttctcgc
     3481 cgaatgaagg cttcaagcgc aacgagaaaa atccgctcga catcgatatc ttgatcgtcg
     3541 acgaagccag catgatcgat ctattgttga tgaatcattt gctcaaagcc gtgccgatcg
     3601 atgcgcatct gctgctcgtc ggcgatatcg atcaattgcc cagcgtcggc gctggggatg
     3661 tgctgcggga catcatcgat tctggccgcg ccgcagtgat tcgactggat gtgatttttc
     3721 ggcaggcgca gaactcacac atcattacca attcacatcg catcaatcaa ggccagatgc
     3781 cgctgacacc cgcagaggcc gatgattttt tcttgttcgt taaagaagag cccgatgagg
     3841 ccgcggattt gatcgtggat ttggtcgcca cccgcattcc cgccaagttc ggcttgaaga
     3901 tcaatgacct gcaagtttta tcgccgatgt accgcggcag taccggcgtc tccgcattga
     3961 atgccaagtt gcaagagaaa ttgaatccac cctccgccaa gaaaattgaa cggaggttgg
     4021 gcggcacact cttccgcgtc ggcgatcgcg tgctgcaaac gcgcaacaac tacgacaaag
     4081 atgtttacaa tggcgatatg ggttatgtga ccgcgatcga tctggagaat caaacgctca
     4141 ccgtgtcgat cgatggtcga ccgatcgagt acgattggag cgaggctgac gaagtagtgc
     4201 tggcgtatgc gatcagcgta cacaaagcgc aaggcagcga atatccggcc atcgtgatgc
     4261 cgctgttgac ccagcattac atgctgctgc agcgcaatct actctatact gccatcacgc
     4321 gcgcgaagaa actggtcgtg ctcgtcggca gtcggcgcgc tttagcgcta gccgtgaaga
     4381 acaataagcc gagtgaaaga ttttctggct tgaaagaaag attatcgtcg gtgtaggggc
     4441 gagccgattg tgccctccag tctattccgg acttccaaat cttcaatacg ccgccataat
     4501 ccttgaatca cttgcagcat ctgcccgaca gccagatcgg gtgtgaggtc ttgcgtgaac
     4561 ccgcgccgaa agagttcttc cacgccatat taccggtgag aagtcaatga g
//
LOCUS       DASRTJ010000533         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_165842,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000533 DASRTJ010000000
VERSION     DASRTJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165842"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1071)
                     /locus_tag="VFF70_10835"
     CDS             complement(1..1071)
                     /locus_tag="VFF70_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY45233.1"
                     /translation="MSTPEIDAELTEPCPHCDEEMPEDLNICPHCGGERCFHCGAAID
                     ANDLQCAECGTVFVLMCPQCGHEVPDGAQKCPNCGLIFKVSNEEGDHCPHCGAATNFS
                     DGVCSVCGLTFCPRCGTSTQEADRTCGNCGLELYFNCPDCSIELLASTPICPSCDRLF
                     TRGCPHCHQAIYGAPIDCPHCHNALPAEKASIESAKPRVGSILTTYNLIDCPNCKHRF
                     DPNSGPCPNCGTIICPKCHALAFEGETNCLRCGTELPVTVSTCPRCQQEVSDDAETCP
                     NCGLDLSGKCPRCKFDLPDNADHCPNCGQSVCPECGSATSEDAVQCSVCGAQFSFCCP
                     ECGHDVPADALSCPNCHTVFET"
     gene            complement(1072..1692)
                     /locus_tag="VFF70_10840"
     CDS             complement(1072..1692)
                     /locus_tag="VFF70_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZY45234.1"
                     /translation="MYTPTHFRIEDRSVLFDFISKNNFGILVTIDHSTPIATHLPFLI
                     EPDRGERGTLIGHIAKANTQWQSFSSQSEALVIFQGAHTYISPSWYQSQPSVPTWNYA
                     TIHAYGVPRIIDDAHATHNMLGGLVNKHEAAFEQPWEMKLPDDYYRQQTNAIVVFEIE
                     LMRLEGKFKMSQNRSAEDRSGVIAALEQSGDDNSIEVAHSIKQASR"
     gene            1949..2512
                     /locus_tag="VFF70_10845"
     CDS             1949..2512
                     /locus_tag="VFF70_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY45235.1"
                     /translation="MSEPDSDLDLVARVKQDPAVFGVLYERYITKIYNYVYYRTGNHH
                     DAEDLTSRVFYRALMHVEEYTDRGVPFSAWLYRIAHNLVANWHRDHARRPETNLDDVV
                     LIGPPREMPEPATEANEKRKWLLAAIRLLPGERQELLILKFVEQLSNAEIGRIMGRSE
                     GAIKSLYHRTLLSLRDELMGIDLINEE"
     gene            2502..2885
                     /locus_tag="VFF70_10850"
     CDS             2502..2885
                     /locus_tag="VFF70_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45236.1"
                     /translation="MKNKLSVSRSSRKKVVPIDDAMLEQWLAEALDVPSSKLKPIDAR
                     TQAILSVVDSVRDTLVPILPSPHFVHDLRDRLVAKTSQGKQSLLQRYRTAIVIGVAAA
                     GSVASIVGVTALLLHQKNRRSVRGV"
     gene            3135..>4283
                     /locus_tag="VFF70_10855"
     CDS             3135..>4283
                     /locus_tag="VFF70_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZY45237.1"
                     /translation="MTRTLAAKTYARIPDVHQLPNLIEIQLNSFNWFINQGLRELFDE
                     VSPIDSFNGSLKLYFPGDSPEAKQFGLSFWFEDPKFNQKECLERDLTFARGLYIKVAL
                     VNKETNEHVVNDIFLGEFPWMTENGTFIYNGTERVVVSQLIRSPGVYFDADPDLSSGR
                     VLANAKVIPDRGAWMEFETRKSDYITLKFNRKRTIPVTILLRALAAIDDGSSTDLLKT
                     GQDDEILQLFEHIDNNPDHQYILTTIRQEGNLEPRDKRSIAEEALLEFYRKMRPGDPP
                     TLDNARSYLRDQLFDRRRYDLERVGRYKLNQRLGVNQQIRTVTKVDLIKVVEHIIRIN
                     NGLETVDDIDHLGNRRVKTVGELIQNKLRIGLRRMERVVKERMSIREAD"
BASE COUNT         1027 a         1083 c         1155 g         1018 t
ORIGIN      
        1 tcaagtttca aatacggtgt ggcaattcgg acacgacaat gcatccgccg ggacatcgtg
       61 gccgcattcg gggcaacaga atgaaaactg cgcgccgcag accgagcatt gaaccgcatc
      121 ttcactcgtc gccgaaccac attcggggca aacggactga ccacagttcg gacagtgatc
      181 ggcgttgtcg ggtaaatcga atttgcagcg tgggcacttg cccgatagat cgagtccgca
      241 gttagggcaa gtctcggcat catctgatac ttcttgctga caacgcgggc aagtagagac
      301 agtgacgggt aactctgttc cgcagcgcaa gcaattcgtt tcgccttcaa aagcgagggc
      361 gtggcacttg ggacaaatga tcgtgccgca atttgggcag gggcctgaat ttggatcgaa
      421 ccggtgtttg cagttggggc aatcaatcag gttgtacgtc gtcaagatcg atccgacgcg
      481 cggcttggct gattcgatcg acgctttttc ggcaggcagg gcgttatggc agtggggaca
      541 atcgatgggc gcaccgtaga tcgcttgatg acagtgtgga catccgcgcg tgaagagtcg
      601 atcgcacgaa gggcagatcg gtgttgaggc cagcagttcg atcgagcaat ccgggcaatt
      661 gaaatacagt tccaggccgc agttgccgca agttcgatcg gcttcttgtg tcgatgttcc
      721 acagcgcggg caaaacgtta aaccgcagac actgcacaca ccatcactga agtttgtcgc
      781 cgcgccgcag tgcgggcaat gatcgccttc ttcattagat actttgaaga tcaatccaca
      841 gttcgggcac ttctgcgcgc catcgggcac ttcgtggccg cattgcgggc acatcaacac
      901 gaaaaccgtg ccacattcag cgcactgcaa atcgttggca tcaatcgccg cgccgcaatg
      961 aaaacagcgt tcgccgccac agtgcggaca gatatttaga tcttcgggca tttcctcgtc
     1021 gcagtgcgga cacggttcgg ttagctctgc atcgatttca ggtgtggaca tttagcgact
     1081 cgcttgtttg atcgagtggg caacttcaat cgagttgtca tcacccgatt gttcaagcgc
     1141 agcgatgacg cccgatcgat cttcggccga tcggttctgg ctcattttga atttgccctc
     1201 gaggcgcatt agttcgatct cgaagaccac gatcgcgttg gtttgttggc ggtaataatc
     1261 gtcaggcaac ttcatttccc agggctgctc gaaagcggct tcatgtttgt tcactaagcc
     1321 gccgagcata ttatgagtgg catgcgcgtc atcaataatg cgcggaacgc cgtaagcatg
     1381 tatggtggca tagttccagg ttggcacact cggctgagat tgataccatg acggcgagat
     1441 ataagtgtgt gcgccctgga agatcactaa agcttcgctt tgagacgaga aagattgcca
     1501 ctgcgtattt gctttggcga tgtgaccgat caatgtgcct cgctcacctc gatcgggctc
     1561 gattaaaaag ggcaggtggg tcgcgatggg cgttgaatga tcgatcgtga ccagaatccc
     1621 gaaattattc ttcgagataa agtcgaacaa caccgatcga tcttcaattc gaaagtgggt
     1681 gggggtatac acgcgaaatc gccatcgaaa attaaggtaa gcatcctgag cggaattgcg
     1741 gcctcgaaga gatcgcaacg cagtcgatga ggcgttgcac tgatccgctc cttcgactgc
     1801 gagttgaacc cgccgagttc aactctttcc tcacggcgct ttgccatttt taattctaac
     1861 acgaaagcag ttgacaattt ttgcagcaga ctgctatact cagctgcagg aacacgcaac
     1921 tttttgcgcg cgatggtgtt ttcatttaat gagtgaacct gacagcgatc tcgatttggt
     1981 ggcgcgggtc aaacaggatc cggcagtgtt cggcgtgttg tatgaacgct acatcaccaa
     2041 aatctacaac tacgtttatt atcgcaccgg caatcatcac gatgcggaag atttgactag
     2101 ccgtgtgttt taccgcgcgc tgatgcatgt ggaagagtac accgatcgag gtgtgccgtt
     2161 ttcggcgtgg ctgtatcgca ttgcgcataa cttagtagcg aactggcatc gtgatcatgc
     2221 gcggcgacca gaaaccaatc tagatgatgt ggtgctaatt ggcccgccac gggaaatgcc
     2281 ggagccggcg actgaagcga atgagaaacg taaatggctg ctggcagcga tccgactttt
     2341 acccggcgag cggcaggaat tattgattct caaatttgtc gagcaactgt ccaatgcgga
     2401 gatcggccgg atcatggggc gcagtgaagg ggctatcaag tctctgtacc accgtaccct
     2461 attgtccctg cgcgatgagt tgatgggaat tgatttgatc aatgaagaat aaactttcag
     2521 tctctcgatc ttctagaaaa aaagtagtac ccatcgacga tgcgatgctg gagcagtggc
     2581 tcgccgaggc gttggatgtt ccatcgagta aactcaagcc gatcgatgcg cggactcaag
     2641 cgatcctgtc ggtggtcgat agtgtgcgcg atacgctcgt cccaatccta ccttcaccgc
     2701 attttgtcca cgatcttcga gatcggctcg tcgcgaagac cagccaaggt aaacaatcat
     2761 tgcttcagcg ttatcgtacc gcgatcgtta tcggcgttgc cgccgccggc tcggttgcgt
     2821 caattgtagg cgtcaccgcg ctgttgctgc accagaaaaa tcgacggagt gtgcgtggcg
     2881 tttagcactt tagacctaag cggtaacgtg cgtgaataaa aattcatcgt gcgttttggg
     2941 agttaatcta gcgaagatcg gcttggggca tcgggagcag aaaaacctcg atcgggttgt
     3001 tgacagaatc ctcatcacac tgtacaatat tcttttgcta ctcgcgaaat actattaatt
     3061 aacgctattc atacagggga ttgtcatccc ctttgttgcg tttaatggct taattgacag
     3121 ataggagctc gtgaatgact cgaacccttg ccgccaagac ctatgcccgc attcccgatg
     3181 tacatcaact acccaacctc atcgaaattc aactcaactc ctttaactgg tttatcaacc
     3241 agggattgcg tgaactcttc gatgaagtat cgcccatcga cagtttcaat ggctcgctga
     3301 aattatactt tccgggcgac agccctgaag ccaagcaatt cggactgagt ttttggttcg
     3361 aagatcccaa gttcaaccag aaggaatgcc tggagcgtga tctcacgttt gcgcgcggtc
     3421 tctacattaa agtcgcatta gtcaacaaag agaccaacga gcacgttgtc aacgatatct
     3481 ttctcggtga attcccgtgg atgaccgaga acggcacttt catttataac ggcactgagc
     3541 gcgtcgtcgt atcgcagttg atccgatcgc ccggtgtgta tttcgatgcc gatcccgatc
     3601 tatcatctgg ccgggtgttg gccaacgcca aagtcattcc cgatcgcggc gcgtggatgg
     3661 aattcgaaac gcgtaagagc gattacatta ccttaaagtt caatcgcaag cgcacgattc
     3721 cggtgaccat cttgttgcgc gctttggccg ccatcgacga cggatcctct actgatcttt
     3781 tgaagacggg tcaagatgac gagattcttc agctattcga gcacatcgac aacaaccccg
     3841 atcatcaata tatcctaacg actatccggc aggaaggcaa cctcgaaccg cgcgacaagc
     3901 gatcgatcgc ggaagaggct ttgctcgaat tctatcggaa gatgcgcccg ggcgatccgc
     3961 caacattgga caacgcgcgc agttatttgc gtgatcagct gttcgatcgg cgccggtatg
     4021 accttgagcg cgtcggccga tacaagttga atcagcgctt gggggtcaat cagcagatcc
     4081 gcaccgtcac caaagtcgac ttaatcaaag tcgttgagca catcatccgg atcaacaacg
     4141 gtctggagac ggtagacgat atcgatcact tgggaaatcg tcgcgtgaag acggtgggcg
     4201 aattgattca gaataagctg cgcattggtt tgcgccgcat ggagcgggta gtcaaagaac
     4261 gcatgagcat ccgcgaagcg gac
//
LOCUS       DASRTJ010000534         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_355113,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000534 DASRTJ010000000
VERSION     DASRTJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355113"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFF70_10860"
     CDS             complement(<1..1220)
                     /locus_tag="VFF70_10860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZY45238.1"
                     /translation="MKKLRKWIVLGVVVVVIAAAAVIITNNQRSAAQAAALANVQFGR
                     VTRATLLSTIDSSGSVSPESKLTLSFGSAGTVAKVNVQPGDRVKKGDVLAQIDTTSLE
                     LQVAQAQQSLLIQQASYSMTIQPDPAAITSALLSLSSASANYKLAQQKFAVSNTDQVY
                     VSCNGLDNAKRSYDDAVTAYNMLLANWRAVVNGSYLASPQKSQLDRAKANYDQAVSTC
                     DSAKSNVNDSSVKSAYASYLQAKTNYETLLTPSERTLVSAQLQLAQAESAYEQAQQNL
                     ADAAIVAPLDGVVTQVNATVGGPAVSSGVVLLADVSHYHVNVFVDETEIGQVKVGQKV
                     QVTFDALVNVNSACTVKQISPAGTISQGVVNYLVRVDLDPLTATLRLDMTSNVRVVID
                     THANVLAVPGGAVRS"
     gene            complement(1298..2047)
                     /locus_tag="VFF70_10865"
     CDS             complement(1298..2047)
                     /locus_tag="VFF70_10865"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZY45239.1"
                     /translation="MTLKRILIGVVALAAVGGIGLFILDRTIFAPVTVSSNLPAAPTL
                     AAPTAAAAHDLTSAASADSTPLATSGQLYRIDAAQSEVHYEVDETLFNENNRLNTAIG
                     RTKGIAGEIRVDFAQPANSQIGTFVIDVSQFASDESRRDNFIRRQGLESGRYPTATFT
                     TRSIDGLPAQVQPGQSINFTITGDLTVKTTTKQVTWSVSLTADKDKLIGSATANIKLS
                     DFAAGPIQIPFLQTKDDAKLVFDIVAAAATS"
     gene            complement(2230..2397)
                     /locus_tag="VFF70_10870"
     CDS             complement(2230..2397)
                     /locus_tag="VFF70_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45240.1"
                     /translation="MLEKVYGFSATWLRTDYQFQSVVEAVELTRFFFGIEFGDRVQQH
                     QLTVLPECTGL"
     gene            complement(2413..2913)
                     /locus_tag="VFF70_10875"
     CDS             complement(2413..2913)
                     /locus_tag="VFF70_10875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY45241.1"
                     /translation="MTDFKSIYLNQAAQFDRLVTREDYHYNSLCALNHIRSMQSADIV
                     VLGAGTGRLTLMVAPIARSILVTDASQHMLEVAAQKLRVSSSRNWQVVVGDNRALPLP
                     DRSAEVSIAGWSLGHTCGWYPNTRRQEIDRAIQQMRRVLRRDGLSIILETLGTECELP
                     HPPSET"
     gene            3126..4292
                     /locus_tag="VFF70_10880"
     CDS             3126..4292
                     /locus_tag="VFF70_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZY45242.1"
                     /translation="MRLSFRRMAGFLVIGLLIELSFATGLLLSPKLISRPQTSAAVAP
                     RTLFDEAWSLAESQFFGALPAEQARTYAAIHGMISAYHDPNTIFAEPPATELQGQQLS
                     GKFGGIGANVRRGADGQLVLSPFPDQAAAKAGVHEGDVLVRVDGNIISTTTPLNDILA
                     WLRGDVGTTVTLEVARATQQLFFTIARTEINTPSVTWRILTEAADTGYIAISVFSQTT
                     SDELVKAINDLRQHGAQRLILDVRDNGGGLFNAAIDVASQFVEGTIVIETHRDKTETP
                     FAVKSIGAARDLPVVVLVNHNTASAAEIVAGAIQAKRRGVLIGDQTYGKGSVQNILPL
                     SDGSSLHVTVAEWLTPDRNQISGTGLTPDVVVARTLEDAQANRDPQLDRAVEYR"
BASE COUNT         1046 a         1245 c         1094 g          994 t
ORIGIN      
        1 gatcggactg cgccgcccgg caccgccagc acattcgcat gcgtatcgat caccacccgg
       61 acattggagg tcatatccag tcgcaacgtc gcagtcaacg gatcaagatc gacccgcacc
      121 agataattca cgaccccttg actgatcgtg ccagcgggcg aaatctgctt gaccgtgcaa
      181 gccgaattga cgttcaccag tgcgtcaaat gtaacctgca ccttctgccc gaccttgact
      241 tgaccaattt cggtctcatc cacgaatacg ttaacgtgat aatggctgac atccgccagc
      301 agcaccacgc cgctgctgac cgccgggccg ccgaccgtgg cattcacttg cgtgaccact
      361 ccgtccagtg gcgccacgat cgcggcgtca gccaaatttt gctgagcttg ctcgtaagcc
      421 gattcggcct gcgccaactg caattgagcc gagaccaacg tccgttcgga cggagtcagc
      481 agcgtctcat aattggtctt cgcttgcaag tacgaggcat aggccgattt gacgctgcta
      541 tcattcacgt tgctcttcgc cgaatcgcag gtcgacaccg cttgatcgta attggccttc
      601 gcccgatcga gctgggattt ttgcggcgag gccagatacg atccattcac caccgcccgc
      661 caattagcca gtaacatgtt gtaggccgtc acggcgtcat cataacttct cttagcattg
      721 tccagcccgt tacaactgac gtacacttga tctgtgttgc tcaccgcaaa cttctgctgc
      781 gccaacttgt aattggccga ggcactgctc aacgacagca gcgccgacgt aattgccgcc
      841 ggatccggtt gaatggtcat gctgtaggat gcctgctgaa tcaagagcga ttgctgggct
      901 tgcgcgactt gcaattccag actggtcgta tcgatctgcg ccagcacatc gcctttcttc
      961 acacgatcac ccggctgcac attgacctta gcaactgtgc cggccgatcc aaaggacaag
     1021 gtcaatttcg attcagggct gacgctgcca gacgagtcga tggtcgataa caacgtcgcg
     1081 cgtgtgacac gtccaaactg aacattagcc aacgccgcgg cttgagcagc gctgcgctga
     1141 ttattggtga tgatcacggc cgcagctgca atcacaacta ccactacccc cagaacgatc
     1201 cactttctta gtttcttcat tgattagtac tcccaagcat tattagaatg cagcccattt
     1261 cgttttaagc cggcgcttag cgccacatcg gcatcgatca gctcgtcgct gctgcagcca
     1321 cgatatcaaa caccaacttc gcatcatctt tagtctgcag gaatggaatc tgaatcggcc
     1381 cggccgcaaa atcagaaagt ttgatattcg ccgttgcgga accgatcagt ttatctttgt
     1441 ctgccgtcaa gctcacgctc caagttactt gcttggtcgt cgtcttcacc gtcagatcgc
     1501 ctgtgatcgt aaagttgatc gattggccgg gttgaacctg cgccggtaag ccatcaatcg
     1561 atcgagtggt gaaagtagcg gtgggataac gtcccgactc caagccttgc cgccgaatga
     1621 agttatcgcg ccggctttca tctgacgcaa attgactcac atcgataacg aatgtcccaa
     1681 tttggctatt agctggctgg gcaaaatcaa cccgtatctc acctgcgatc ccttttgtcc
     1741 ggccaatcgc tgtattcaat cgattgttct cattgaatag cgtttcatca acttcataat
     1801 gcacttcgga ttgagcagca tcaatgcgat agagctgccc ggatgtcgcc agaggcgtag
     1861 agtcagctga ggccgccgat gtaaggtcat gcgcggcggc tgcggttggt gccgccagtg
     1921 ttggggcagc cggcaaattg gaactcactg tcaccggcgc aaagatcgtt cgatcgagaa
     1981 taaacaaccc aatgccacca acagcagcga gtgccacaac cccaatcaaa atacgtttca
     2041 acgtcataac aagtctcctc tgctaaatca attctaaagc ctcatctatt ctaatcgctt
     2101 tacttccgca aggaagtgac taaggtcatt acttgaactt aatcacaccc tctgtggact
     2161 tgccagccat gacttccggc atggatcatg tttaaacgct aatgatcgat taatctacca
     2221 gaggcgccat cacaagccgg tgcattctgg taaaaccgtc aattgatgtt gctgaacccg
     2281 atcgccaaac tcgatcccaa agaaaaatcg ggtgagttcg acggcttcaa ccacagactg
     2341 aaattgataa tcggttcgca accatgtcgc tgaaaaacca taaacttttt ccaacaccca
     2401 ataataagca gctcatgttt ctgatggcgg gtgcggcaat tcacactcgg tgcccaatgt
     2461 ttctaaaatg atcgatagac catcccggcg cagcacacgc cgcatctgtt gaatcgcccg
     2521 atcgatctcc tgccgccgcg tgttcggata ccagccgcaa gtgtggccta agctccaacc
     2581 tgcgatcgat acttcagcag atcgatcggg gagcggcaac gcccggttat cacctacaac
     2641 aacttgccaa ttccttgatg aggatactcg cagtttttgc gctgccactt ccagcatatg
     2701 ttgcgatgcg tcggtcacta aaatcgaccg agcgatcggg gcaaccatca aggttaagcg
     2761 gcccgtgcct gcacccagta cgacaatgtc agccgattgc atagatcgaa tatgattcaa
     2821 cgcgcatagg ctattatagt gataatcttc gcgcgttacc aatcgatcga attgcgcggc
     2881 ttggttcaga tagatggatt tgaagtcagt catgtcgggc aagatgccat cctgtcttaa
     2941 aaattacttt tggcaagacc cgcttacagt cgcacccgcg aaagcgggaa tccaaatcat
     3001 aatcacttga cacccgcctt cgcgggtgcg gcactgggta atgtaaggta caagtataac
     3061 aaaaaccttg tcggtgctaa tcatgtgtgt tagaatcatt tgggagtgag cggtctacca
     3121 aacttatgcg actttcattc cggcggatgg ccgggttttt agttattggc ctgctgatcg
     3181 agctcagttt cgccaccggg ctgctgttgt cgccgaagtt gataagccga ccacaaacct
     3241 ccgcagctgt tgccccacgc acgctgtttg atgaagcctg gtcgctggcc gagtcgcaat
     3301 tcttcggagc cttgcccgcc gagcaggcac gcacgtacgc cgccattcat ggcatgatca
     3361 gcgcttacca tgatccgaat acgatcttcg ccgaaccgcc cgccaccgaa ttgcaaggcc
     3421 aacagctcag tggcaagttc ggcggcattg gcgcgaatgt gcggcgcggg gcggacggac
     3481 agctagtgtt gtcgccgttc ccggatcaag ctgcggccaa agccggcgtg cacgaaggcg
     3541 atgttttagt cagggttgac gggaacatca tcagcacgac aacaccgctt aatgatatct
     3601 tagcctggct acgcggcgat gtcgggacga cagtcacgct cgaagttgca cgggccacac
     3661 aacaattatt ttttaccatc gctcgcaccg agatcaacac gccgtcggtg acctggcgga
     3721 tattgaccga ggcagccgat acgggttaca tagccattag tgtcttctcg caaacaacca
     3781 gcgacgaact cgttaaagcg atcaacgatc tgcggcagca cggcgcgcaa cgtctaattc
     3841 tcgatgtgcg tgacaacggt ggcggtttgt tcaacgccgc gatcgatgta gctagccaat
     3901 ttgtcgaggg cacgatcgtc atcgagactc accgtgataa aactgaaacg ccgttcgcag
     3961 tcaaatcaat cggggccgca cgcgatctgc ccgttgtcgt gttggttaat cacaacacag
     4021 ccagcgccgc cgaaatagtc gccggggcca tacaggcgaa gcggcgcggc gttttgatcg
     4081 gcgatcaaac ctacggcaaa ggatcggtgc agaatatttt gccgctgtca gatggatcat
     4141 cactgcatgt cacggtcgcc gaatggctga cccccgatcg aaatcagatc tcgggcacag
     4201 gcttgacacc tgacgtggtg gtcgcccgta cactggaaga tgcacaagcc aatcgtgacc
     4261 cgcagctcga tcgggcagtt gagtatcgat agaagtaaga ggttagaagt tggaagttgg
     4321 gtgaagcatt gagacacaca ctccaccttc caatctccaa tttcaaatca caaggagag
//
LOCUS       DASRTJ010000535         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_127769,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000535 DASRTJ010000000
VERSION     DASRTJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127769"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="rplO"
                     /locus_tag="VFF70_10885"
     CDS             complement(<1..282)
                     /gene="rplO"
                     /locus_tag="VFF70_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZY45243.1"
                     /translation="MKLHELKPGDGSKHRAKRVGRGLGSGHGTYSGRGKKGQRSRSGG
                     TKAPYHQGGNLPLVRKLPFVRGVGFTSRNKIDYAPVNVGHLVDFKAGSDV"
     gene            complement(279..494)
                     /gene="rpmD"
                     /locus_tag="VFF70_10890"
     CDS             complement(279..494)
                     /gene="rpmD"
                     /locus_tag="VFF70_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZY45244.1"
                     /translation="MPKKPNIAKSAGARLRITLVKSPVGNMKRHKQTVRALGFHRLSE
                     TVEQPDTLVTRGMIAKVAHLVMVEEVK"
     gene            complement(478..1029)
                     /gene="rpsE"
                     /locus_tag="VFF70_10895"
     CDS             complement(478..1029)
                     /gene="rpsE"
                     /locus_tag="VFF70_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZY45245.1"
                     /translation="MAKRSDRRERNDEPREELDERVVNITRVAKVIKGGRRFAFRVVI
                     VVGDNRGTVGVGVGKSRGVPEAIRKATERARRAMHPVALAGTTIPHAITSKFGGAKVL
                     LKPASPGTGVIAGGGIRAVLEAAGVRDILTKSLGSSNTLNMIRATMQGLEQLKTPEFV
                     AKTRGKNVEDLLPFWRRRNAQKA"
     gene            complement(1039..1401)
                     /gene="rplR"
                     /locus_tag="VFF70_10900"
     CDS             complement(1039..1401)
                     /gene="rplR"
                     /locus_tag="VFF70_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZY45246.1"
                     /translation="MGKSRSELRQRRQRRVRGKVNGNAARPRLSVYRSLMNIYAQLID
                     DEQGHTLASASTIDPEVAKQITGLNGVAKAKLVGKVLAERAKQAGIQTVVFDRGGYRY
                     HGQVKALADASREAGLHF"
     gene            complement(1470..2018)
                     /gene="rplF"
                     /locus_tag="VFF70_10905"
     CDS             complement(1470..2018)
                     /gene="rplF"
                     /locus_tag="VFF70_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZY45247.1"
                     /translation="MSRIGKKPVPVPAGVQIKIDGSNVSIKGPRGELSRSFHPAMKVK
                     QEGPELHVVRPDDQRQNRALHGLTRALLNNMVEGVTHGFKKTLIIEGVGYRAELAGKS
                     LMLYLGYSHPILIEPPDGIKFEADIKAKTVTIDGNDRELVGEVAAEIRAARPPEPYKG
                     KGIRYVGEVIRRKAGKAGKVGA"
     gene            complement(2059..2472)
                     /gene="rpsH"
                     /locus_tag="VFF70_10910"
     CDS             complement(2059..2472)
                     /gene="rpsH"
                     /locus_tag="VFF70_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZY45248.1"
                     /translation="MSISDPIGDMLTRVRNATMVGHSVVAMPSSRMKLAIAHILKEEG
                     FIQDFEAALEQGHVQPTLRVWLKYVGDRRERVSAITGVERISRPGCRIYTHKEDVPWV
                     LSGMGIAILSTPKGVITGQQARRLGVGGEVLCKVW"
     gene            complement(2597..2779)
                     /locus_tag="VFF70_10915"
     CDS             complement(2597..2779)
                     /locus_tag="VFF70_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZY45249.1"
                     /translation="MAKKSMINREQHRKYAVRVRHRCSKCGRGRGYMRRFDLCRICFR
                     ELALQGMVPGVTKSSW"
     gene            complement(2785..3330)
                     /gene="rplE"
                     /locus_tag="VFF70_10920"
     CDS             complement(2785..3330)
                     /gene="rplE"
                     /locus_tag="VFF70_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZY45250.1"
                     /translation="MPNIQQQYKTELVPTLMKEFNYTSVMQVPRLTKVVVNIGAGAAM
                     DNPKILDGAVADLLLITGQKPIITKARTSIATFKLREGRQIGVKVTLRGARMWAFVDR
                     LCNIALPRLRDFRGISADAFDGRGNYTLGLREQLAFPEIQYDKIDMLRGMEITLVTTA
                     KTDAEGRRLLALIGMPFVRSN"
     gene            complement(3341..3658)
                     /gene="rplX"
                     /locus_tag="VFF70_10925"
     CDS             complement(3341..3658)
                     /gene="rplX"
                     /locus_tag="VFF70_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZY45251.1"
                     /translation="MANIKKGDTVQVITGEAAGVRGLVHRIDAKNERVVVAGANLVKK
                     HQKPTGNKRTQVGIIEFEAPIHLSNVMIVCPHCNELTRVSYEIDPDGHKARVCKNGDR
                     LDS"
     gene            complement(3728..4099)
                     /gene="rplN"
                     /locus_tag="VFF70_10930"
     CDS             complement(3728..4099)
                     /gene="rplN"
                     /locus_tag="VFF70_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZY45252.1"
                     /translation="MIQQETRLTVADNTGAKELLCIHVLGGSTRHYGYVGDVIVATVK
                     KAVPNSAVKKGDVVKAVIVRTAKEYIRSDGSHIRFDDNAAVLIEAETLNPRGTRIFGP
                     VARELRDKGFMKIVSLAPETL"
     gene            complement(4102..4356)
                     /gene="rpsQ"
                     /locus_tag="VFF70_10935"
     CDS             complement(4102..4356)
                     /gene="rpsQ"
                     /locus_tag="VFF70_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZY45253.1"
                     /translation="MNKQRKRLVGRVTSDKMQKTVVVTVDQLKRHRLYSKVIRRSHKY
                     KAHNPGDVAKTGDLVQIVEAQPLSREKRWAVESILEKAQA"
     gene            complement(4353..>4538)
                     /gene="rpmC"
                     /locus_tag="VFF70_10940"
     CDS             complement(4353..>4538)
                     /gene="rpmC"
                     /locus_tag="VFF70_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZY45254.1"
                     /translation="TQEIQHKLAASHQELFNLRFQWASGRLEDHNRITMLRRDVARMQ
                     TVLHERELAALAQGESK"
BASE COUNT          948 a         1347 c         1121 g         1122 t
ORIGIN      
        1 cacgtccgat cccgctttga aatcgactag atgtccgaca ttcaccggcg cataatcgat
       61 cttattgcgc gaggtaaagc cgacgccgcg cacaaagggc agcttgcgca ctaagggcaa
      121 gttgccaccc tgatgataag gggctttggt gccgcccgat cgggagcgct ggcccttctt
      181 gccgcggcca gaataagtgc catgccctga accaagaccg cggccaacgc gcttggcccg
      241 gtgtttcgat ccgtctccgg gttttaattc atgcagtttc atttgacttc ctcaaccatc
      301 accagatgcg ccactttggc aatcatgcca cgcgtgacca gtgtatccgg ctgctcgact
      361 gtttcgctca ggcggtgaaa gccgagcgcg cgtacggttt gcttatggcg tttcatattt
      421 ccaaccggac tcttgaccaa agtgatgcgc aatcgcgcac ccgctgattt agcaatgtta
      481 ggctttttgg gcattgcggc gtctccagaa tggcagcaaa tcttcgacgt tcttgccgcg
      541 cgttttggcg acaaactccg gggtcttgag ttgttcaagc ccttgcatag tcgcgcggat
      601 catattcaag gtattgctgc tgcctaatga cttcgttaaa atatcacgga caccggccgc
      661 ttccagtaca gcacgaattc cgcccccggc aatgacaccg gtgcccggtg aagcgggctt
      721 caacaaaact tttgcgccgc caaatttact tgtaatcgcg tgcgggatcg tcgtgcccgc
      781 taacgcgaca ggatgcattg cgcgccgcgc acgttcagtc gcttttcgga ttgcttccgg
      841 cacaccgcga ctcttgccaa caccgacacc gacagtaccg cgattgtcac cgacaacaat
      901 caccacgcgg aaggcaaagc ggcggccgcc tttaatgact ttggccacgc gcgtaatgtt
      961 gacgactcgc tcgtccagtt cttcgcgcgg ctcatcgttt cgttctcgtc tatcagatcg
     1021 tttcgccatg tatcgctcct aaaagtgcag gccggcttcg cgactagcat cagctaaagc
     1081 tttgacttga ccgtgatagc ggtagccgcc acgatcgaat acgactgttt gaattccggc
     1141 ttgcttagcg cgttccgcta acactttacc caccaatttc gctttggcta caccattcaa
     1201 gcccgtgatc tgcttagcta cttccggatc gatcgtggaa gcagaggcga gtgtatggcc
     1261 ttgctcgtcg tcgatcaact gagcatagat attcatcaag ctgcgataga cgctcaagcg
     1321 cggccgtgcc gcgttgccat tgactttgcc gcggacgcgg cgctgccggc gttgtcgaag
     1381 ttcactgcgt gacttgccca tagtccaatc cttgtctcat caaacttccg aagtctcgct
     1441 gactttttgg aagccccaag aacttagtgt tacgcgccaa ccttgccggc cttgcccgct
     1501 ttgcggcgaa tgacctcacc gacgtagcgg atacctttac ctttgtacgg ctctggcggt
     1561 cgagcggctc gaatctcggc cgcgacttca ccgaccaatt cacgatcgtt gccatcgatc
     1621 gtcaccgtct tggccttgat atctgcttcg aacttgatgc cgtccggcgg ctcgatcaag
     1681 atcggatgtg agtagcccag atacagcatc aaggatttgc ccgccaactc ggcgcgatag
     1741 ccgacgcctt caatgatcag tgttttcttg aagccgtgtg tcacgccttc gaccatgttg
     1801 ttgagcaagg cgcgtgtcag accgtgcagc gcgcgatttt gccgctggtc gtctggtcgc
     1861 acaacatgca gctccggtcc ttcttgcttg accttcatgg ccgggtggaa cgatcggctt
     1921 agctctccgc gcgggccttt gattgataca ttcgatccat caattttgat ttgaacaccc
     1981 gccgggaccg gcacgggttt tttcccaata cgagacatta cttcctccga tcaaacttat
     2041 cggctcaggc gcgactgatt accacacctt gcacaagact tctccgccga cgcccaagcg
     2101 ccgcgcttgc tggccggtaa tcacaccctt cggtgtagaa agaatggcga tacccatgcc
     2161 cgacaagacc cacggcacat cctctttatg cgtatagatg cggcagcctg gccggcttat
     2221 ccgctcgacg ccggtaatag ccgaaacgcg ttcgcggcga tcaccaacat actttaacca
     2281 gacacgcagc gtaggttgga catggccttg ttccaatgcg gcttcaaaat cttgaatgaa
     2341 gccttcttct tttaggatat gcgcaatcgc caacttcatg cggctgctgg gcatggccac
     2401 cacactatgg cccaccatgg ttgcgttccg cactcgggtc agcatatccc caattgggtc
     2461 tgaaatactc attccacatg ctcctctacg tccggctaag aacggcgtgt tttcgatatc
     2521 ggccgctgat cgattgggtc aagcctgaat tcaactcatg actgacacgt cactcgatca
     2581 cttccgggct aatttcctac cagctggact tcgttacacc gggcaccatg ccttgcagcg
     2641 ccaattcgcg aaagcaaatt cggcacaaat cgaagcggcg catgtaaccg cggccgcggc
     2701 cgcacttgct gcatcgatgc cggacacgaa ctgcatattt gcgatgctgt tcccgattga
     2761 tcattgactt tttagccata tgtcctaatt gctcctaaca aaaggcatcc cgatgagtgc
     2821 taataagcgg cgaccttccg catcagtctt ggcggtggtc accaatgtaa tttccatacc
     2881 ccgcagcatg tcgatcttat cgtattgaat ttcaggaaac gcgagctgct cgcgtaaacc
     2941 caaagtgtag ttgccccggc catcaaaggc atcggccgaa atgccgcgga agtcgcgcaa
     3001 tcggggcaac gcaatgttgc acaatcgatc gacgaaagcc cacatacgcg cgccgcgcaa
     3061 ggtaaccttc accccaattt ggcggccttc gcgcaatttg aaagttgcga tggaggtgcg
     3121 cgctttggtg ataatcggtt tctgtcccgt aatgagcagc aaatccgcga ctgcaccatc
     3181 caatatcttg ggattatcca tcgctgcgcc agcgccaatg ttgacgacga ctttcgtcaa
     3241 acgcggcacc tgcatgacgc ttgtataatt aaattctttc atcaaagttg gaacgagttc
     3301 agttttatac tgctgctgta tgtttggcac agtcctgctc ctaagaatcg agccgatcgc
     3361 cgtttttaca aacgcgcgcc ttgtggccgt ccggatcgat ctcataactc acacgagtca
     3421 actcattaca atgcgggcaa acgatcatca cattcgacaa atgaatcggc gcttcgaatt
     3481 cgataatgcc cacctgcgtg cgcttattgc cagtcggctt ctgatgcttc ttgaccaaat
     3541 ttgcgccggc gacaaccacc cgctcattct ttgcgtcgat ccgatgtacc aagccgcgca
     3601 cgcctgcggc ttcgccggtg atgacttgta cggtgtcgcc ttttttgata ttcgccacgt
     3661 caatcactcc tctcgcccaa gtgtgacaag ttgccgtgta cactacccac agggttcata
     3721 acttgtctta caatgtttcg ggcgctaatg aaacgatctt catgaagccc ttatcccgca
     3781 attcgcgggc gacgggtcca aaaatgcggg tccctctggg attcaatgtc tcggcctcga
     3841 tcaacaccgc cgcgttatca tcgaaacgga tgtgactgcc atccgatcgg atatactctt
     3901 tcgccgtgcg aacaatgacg gccttgacca catcgccctt cttcacggcg ctgttcggca
     3961 cggctttctt aaccgtggcc acaatgacat cgccaacgta accatagtgg cgggtcgatc
     4021 cgcctaaaac gtggatgcac aataattctt tggcaccggt gttatccgcc accgtcaagc
     4081 gtgtctcttg ctggatcatg gttaggcctg agccttttcg agtatactct cgacggccca
     4141 ccgtttttcg cggctcagcg gctgagcttc gacaatctga acaagatcgc ctgtcttggc
     4201 cacatcccct ggattgtgag ctttgtattt gtgtgagcgg cgaatcactt tgctgtacag
     4261 ccgatgccgc tttaattgat ccacggtgac cacgacggtt ttctgcattt tgtcgctggt
     4321 cacacggccg acgagacgtt tgcgctgctt attcatttcg attctccttg agccagcgcg
     4381 gccagttccc gctcatgcaa gactgtctgc atgcgcgcga catcacggcg aagcatggtg
     4441 atccgattgt gatcttccag ccgaccggaa gcccactgaa atcgtaggtt aaataattcc
     4501 tgatgagacg cagccagctt atgctggatt tcttgtgt
//
LOCUS       DASRTJ010000536         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_165893,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000536 DASRTJ010000000
VERSION     DASRTJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165893"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1462
                     /locus_tag="VFF70_10945"
     CDS             152..1462
                     /locus_tag="VFF70_10945"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZY45255.1"
                     /translation="MTESSQSNLARPIAGAVCLAIGLVLVLIYFAQINRLGVATNPTA
                     LADRSSPIVTISLPSIASPSQTIGTPSLAPTSQSTSTSTPTLAPVTNPVPTAENSLTP
                     IPVSNLIPTKIVVTVAPRLTATNVPATIAPPIDLYSTRYRVGLGVGEGERTSGTLLKL
                     APGFYLTWGLQPIANAAAEYFPMVRVLHGQPRQDLSLFTSYAQAHPGLVWLIGNEPDV
                     VWQDNSTPDEYAAVYQAAYVALKAADPSSRIAIAGVSQPTPLRLQYLDRVLAAYQDQF
                     GEAMPIDVWNVHNFILNEQRGSWGIDIPPGFDVDSGLLHTIDQHDDLKIFRQQIIAFR
                     QWMAAHGYRDTELIVSEYGILMPPDYGFDTARVSDFMIRTFDYFSNAADNSIGNPGDG
                     NRLVQRWCWFSLSASDYIAGNLVDFDSGQITLLGQDFITYLQKH"
     gene            1462..>1803
                     /locus_tag="VFF70_10950"
     CDS             1462..>1803
                     /locus_tag="VFF70_10950"
                     /inference="COORDINATES: protein motif:HMM:NF040059.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45256.1"
                     /translation="MHTTLFEALNIDSAHVVAFVGAGGKTSAIWRLSNELIDRDRHVI
                     VAPTTHILEPALPRNSITYLTARPEPDRLIQLIDLAPRVILAAKRDEAADFDSTDDFP
                     PARPIKLRGLEP"
BASE COUNT          403 a          509 c          459 g          432 t
ORIGIN      
        1 gcaagttgta ccgacgccat ccgctaattc cagtggcggc aattcaacac cgatcatttt
       61 aatcgtcggc gggattgcgt tattgatcgt cggcgttttc ttgatctttg gccgatcgaa
      121 gtcgaacgat caaacgaagc cgcaatagat catgactgag tcatctcaat cgaatctggc
      181 gcgaccgatc gccggtgcgg tgtgtctagc gattggcctc gtgctggtgt tgatctattt
      241 cgcacagatc aatcgattgg gtgttgcgac aaacccgact gcgcttgctg atcgatcgtc
      301 gccaatcgtg acgatctcgc taccgtcgat cgcatcgccc agccagacga ttggtacgcc
      361 ttcactcgcg ccgacaagcc aatcgacttc gacgagcaca ccaacacttg cgccagtcac
      421 gaatccagtt ccaacggcag aaaattcact tacgccgata cccgtatcga atttaattcc
      481 gaccaagatc gttgtaaccg ttgcgccgcg cctcaccgca actaatgttc cggcaacaat
      541 tgcgccgccg atcgatttgt actcaacgcg ctatcgagtt gggttgggcg tgggcgaagg
      601 cgaacgaaca tctggcacgt tgttgaaatt ggcgccggga ttttatttga cgtggggtct
      661 tcaaccgatc gcaaatgcgg cggcggagta ttttccgatg gtacgtgtgc tgcacggcca
      721 gccgcgccaa gatttatcgc tctttacgtc atatgcccaa gcccatccgg gtttggtgtg
      781 gctgatcggc aatgaaccgg atgtggtgtg gcaagataat tccacgccgg acgaatacgc
      841 ggcggtctat caggcggcct acgtcgcact caaagccgct gatccctcca gccgaattgc
      901 gatcgcaggc gtgagtcagc ccacgccgct gcgcctgcaa tatcttgatc gcgtgctggc
      961 cgcgtatcaa gaccaatttg gcgaagccat gccgatcgat gtctggaacg tccacaattt
     1021 tattttgaac gaacagcgtg gcagttgggg catcgatatt ccgccaggct ttgatgtcga
     1081 cagcggccta cttcacacga tcgatcaaca tgatgacctc aaaattttcc gtcagcagat
     1141 catcgcgttc cgccaatgga tggcggcaca tggttatcgc gatacagaat tgattgtgtc
     1201 agaatatggc attctcatgc cgcccgatta cggtttcgac actgcgcgcg taagcgactt
     1261 catgatccgc accttcgatt acttctcgaa tgccgcggac aactcgatcg gcaatcccgg
     1321 cgatggcaat cgcttagtgc agcgttggtg ttggtttagt ttatctgcca gcgattacat
     1381 cgcaggcaac ctagtcgatt tcgattcagg ccaaatcaca ctactcggcc aggacttcat
     1441 cacatatctg caaaaacatt gatgcacacc acgctatttg aagcactcaa catcgactca
     1501 gcacatgttg tcgcgttcgt cggcgctggt ggcaaaacca gcgcaatctg gcgattgtcg
     1561 aacgaattga tcgatcgcga tcgacatgtg atcgttgcgc cgacaacgca tatccttgag
     1621 cccgcgctgc cacgcaattc gattacatat ctgacggccc ggcctgagcc cgatagattg
     1681 atccagctga ttgatcttgc cccacgtgtc atccttgctg cgaaacgtga tgaagctgca
     1741 gactttgatt caaccgatga ttttccccca gcccgaccga tcaagttgcg cggacttgag
     1801 cct
//
LOCUS       DASRTJ010000537         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72113,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000537 DASRTJ010000000
VERSION     DASRTJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72113"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..993)
                     /locus_tag="VFF70_10955"
     CDS             complement(277..993)
                     /locus_tag="VFF70_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HZY45257.1"
                     /translation="MLKKILIAFIALALPLLLVVTSIRVIASDWFIHFEYSRPDFPPD
                     QFGFTLEQRTPLALIGMQSVLPDSQGIVLLERATLPDGSPAFNEREIGHMTDVRVLIS
                     KVYPIELIGLALIVILAVLLNRSIHWRDAVPNGLLLGSILTLAILGMLIAYIFVNFDS
                     FFLQFHQLFFQGTTFMFLFTDTLIRLYPVQFWSDAAITIGAMTTVCALFLLIGSIWWL
                     RRLKKANVIASKARQSLDRI"
     gene            complement(1085..2656)
                     /locus_tag="VFF70_10960"
     CDS             complement(1085..2656)
                     /locus_tag="VFF70_10960"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZY45258.1"
                     /translation="MINNQPDQKSAVRNPKLLLAVFFSGLTTLAVELSAFRLFAPTFG
                     TANLVSAVVIGLILLYLAAGYTIGGRWADRSPRAETLYRIMLWGAFLVGLIPFIAQPL
                     LRIARDNLQDLTNFNAALLILAFVVTLILFSIPVTLLGCVSPFAIRLSITDINKAGRV
                     AGRLYAISTAGSFIGSFLPELILLDLIGTRGTFVLLALILIAIGAIGLGRKSIGFVWM
                     PIVLIALNVLIPVSISEIAGTIYQTESAYNYIQVVQRGDTRFLLLNEGQGIHSIYNPN
                     SIQSNGTWDYFTLAPFFNPAPYSPAGLKRLAIVGLAAGTIARSYTTIFGPFPIDGIEI
                     DPKIVQVGQKYFDMNESNLNIIVADGRAALANSPYHYNVVAIDAFRVPYIPWHLTTRE
                     YFLDVRDHLTSDGVLAMNVGHTPNNYDLVDAIARTAQGVFPSVYVVDVTGSFNSVVFA
                     TMQPTSFDNLRANFDAMPHPLIQSTARLALQNEHTVAASGVVFTDDKAPTEQMTNALV
                     LNYLFGLSSGEIKLP"
BASE COUNT          710 a          687 c          824 g          585 t
ORIGIN      
        1 ctcgccgcga tgagtgtctg gccttcggcg agacggaagc gctcaaaggt gcgctcggcc
       61 atttccccgc caccgatttg atgccgatgg ctttagcgca cgcgaggcgg ctgcaaaagt
      121 ttggggcttg atcaacgaaa gagcactaag aaacacgaag gaaaaactaa agcgccttga
      181 tcggtttcga tcgaggcgct ttgtttgttg agtcattgcg agccgcgcag tccttagatg
      241 gcacagcctc taaggatgtg ccacggcgtg gcaatctcag attcgatcaa gagattgcct
      301 cgctttgctc gcaatgacgt ttgctttctt caatcttctc agccaccaaa tacttccaat
      361 caacaagaac aacgcgcaga ctgtcgtcat tgcgccgatc gtgatcgcgg cgtcgctcca
      421 gaattgcacg gggtacaagc ggatcaaagt gtcggtgaat aagaacatga acgtcgtgcc
      481 ttgaaagaac agctgatgaa attgcagaaa gaatgaatcg aagttgacga agatgtaagc
      541 aatgagcatg ccgaggatgg ccaacgtcag gatcgatccg agaagcaggc cgttgggcac
      601 cgcgtcacgc caatgaatcg atcgattaag cagaacggcc agaataacaa tcagcgccaa
      661 gccaatcaat tcgatcggat agactttgct aatcaagacg cgcacgtcgg tcatgtggcc
      721 gatctcgcgt tcgttgaagg cgggcgaacc atcgggcagg gtggcgcgtt caagcaggac
      781 gatgccttgg ctatccggca ggacggactg catgccgatc aaagccagcg gcgtgcgttg
      841 ctcgagcgtg aagccgaatt gatccggcgg gaaatcgggg cggctgtatt cgaagtgaat
      901 gaaccagtcg ctcgcaataa cacggatact ggtgacaacg agtagaaggg gcagggcgag
      961 ggcgatgaat gcgatgagta tcttttttag catatgtatc ctttatttcg atttagagat
     1021 ggagcgatag agggaaagag ggagtctctc attccgtcca tctctcaacc tcgccatcga
     1081 tcgtttaggg cagtttaatt tcacctgatg aaagcccgaa caaataattt aaaacgagcg
     1141 cattcgtcat ctgctcggtc ggtgctttgt cgtccgtgaa gacgacgccg ctggcggcga
     1201 cggtatgctc gttctgcaac gccagccgcg ccgtcgattg aatcagcgga tgtggcatcg
     1261 catcgaagtt cgcccgcaga ttatcaaacg aagtgggctg catggtcgcg aacacgaccg
     1321 agttaaacga cccggttaca tcgacgacat ataccgatgg aaatacacct tgcgcggtgc
     1381 gagcgatggc atcgactaaa tcataattgt tgggcgtgtg accgacattc atcgccagca
     1441 cgccgtcgct tgttaaatga tcgcgcacgt ctagaaaata ttcgcgcgtg gtgagatgcc
     1501 atggaatgta cggcacgcga aacgcatcga tggcgacgac gttgtaatga tacggtgagt
     1561 tggctaaagc ggcacggcca tcggcgacga tgatattcag attgctttcg ttcatgtcga
     1621 aatatttttg gccgacctgc acaatcttcg gatcgatctc aatgccgtcg atcggaaatg
     1681 ggccaaagat cgttgtgtac gatcgcgcga tagtgcctgc cgcgagtccg acgattgcca
     1741 agcgtttcaa tccggcgggc gagtacggcg cggggttgaa gaagggcgct aaggtgaagt
     1801 aatcccaagt gccgtttgat tgaatgctat tggggttata aatgctgtgg atgccttgcc
     1861 cttcgttcag cagcagaaaa cgtgtgtcgc cgcgttgcac gacttgaatg taattgtaag
     1921 ccgattcggt ttgatagatc gtgccggcaa tctcgctgat cgatacgggg atcagcacgt
     1981 tgagcgcgat caaaacaatc ggcatccaca caaacccgat cgattttcgg cccagcccga
     2041 ttgctccgat cgcgatcagg atcaaagcca gcaagacgaa cgtgccgcgg gtgccgatca
     2101 gatcgagcaa aatgagttcg ggtaaaaacg agccgatgaa actcccggcc gtcgagatgg
     2161 catacaatcg accggcgacg cggcctgctt tattgatgtc cgtgatcgaa aggcgaatgg
     2221 caaacggcga gacgcagccg agcagtgtga ccgggatcga aaataaaatc agcgtgacga
     2281 cgaaagccaa gatcaatagc gcggcattga aattggtgag gtcttgcaga ttatcgcgcg
     2341 cgatgcgcag cagcggctga gcgatgaagg ggatgaggcc gacgaggaac gcgccccaga
     2401 gcatgatgcg atacaatgtt tcggcgcgcg gcgatcgatc ggcccagcgt ccgccgatcg
     2461 tgtaacccgc ggccaggtac aacaagatca aaccgatgac gacggccgaa acgagattcg
     2521 cagtgccgaa cgtcggcgcg aacaatcgaa atgcggaaag ttctacggcg agtgtggtta
     2581 gtccgctgaa gaacacggcg agcaataatt tcgggttgcg gactgcggat ttctgatcgg
     2641 gttgattgtt gatcatgatg cgatgcatta taatcgaaaa gaattttcag cgtcattgcg
     2701 agccgcacta cggcgaagca acacttgcac cgcaatgtcc tgcgatggtg catctgcagg
     2761 atgtgccacg gagcagggca ggtgtctccc gatcgatgga ttaaac
//
LOCUS       DASRTJ010000538         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72163,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000538 DASRTJ010000000
VERSION     DASRTJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72163"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFF70_10965"
     CDS             <1..826
                     /locus_tag="VFF70_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZY45259.1"
                     /translation="SDGYIYVADGIDNHRIAKFDSTGKFVMNIGGSSGEESPNPPAGL
                     FREPWDVAAAPDGSIYVADTWNHRTQHFKADGTFIRAWGTYSNTGGQAVGSEGGFFGP
                     RGIAIDATGRVLVADTGNKRIQIFEANGQFISQFGGAGLLAGNLDEPVGIAVDVKGNI
                     IVADTWNGRIQVFDQTGLPLAQWDIDGWLDRDLVGKPYVAVDQQQRVYVADEVGRRVL
                     VFDETGTYLGGFGGFGLDNKGFQLPGGIAVDKDGFIYVVDSDSSRVLKFPPFQVKQ"
     gene            1078..>1816
                     /locus_tag="VFF70_10970"
     CDS             1078..>1816
                     /locus_tag="VFF70_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZY45260.1"
                     /translation="MKLHEYQSKQMFAKYGIPIPKGEVATRPEDARRIAAELGKRVVI
                     KAQVLVGGRGKAGGVKLANNPDEAEKVASQILGMDIKGLTVHKVLVDEAADIQHEIYL
                     AVTLDRVQRRVIMMASAAGGMDIEEVARTAPAKIIRVAIDPFLGLREYQTRQIAAAIG
                     LDRSLVKDFNTIAQGLYQCFIDLDGSLAEINPLIVNGAGKLQAVDGKILTDDNALFRH
                     GDMAVLRDVDEEAPSETEARQRGLSYIK"
BASE COUNT          442 a          417 c          549 g          408 t
ORIGIN      
        1 ctcagacgga tacatctacg tggccgatgg tatcgacaat catcggatcg caaaatttga
       61 ttcgactgga aaattcgtca tgaatatcgg tggctcgagt ggcgaggaaa gccccaatcc
      121 accagcgggc ttgttcagag aaccgtggga cgtggctgcc gcgccggatg gatcgatcta
      181 tgtggctgat acttggaatc accgtacgca gcattttaag gcggacggca ccttcattcg
      241 cgcgtgggga acgtacagta acacgggcgg tcaggcagtt ggttcagagg gtggcttctt
      301 tgggccgcgt ggcattgcga tcgatgcgac tgggcgtgta ttagtagctg ataccggcaa
      361 caagcggata caaatctttg aggctaatgg acaattcatt tcacaatttg gcggcgctgg
      421 tctgctggcc ggaaatctag atgaacccgt gggtattgca gtcgatgtaa aaggcaacat
      481 cattgtcgcc gatacttgga acgggcgtat tcaggtgttc gatcagaccg gattgccgtt
      541 ggcacaatgg gacatcgatg gttggctgga tagagatttg gttggcaaac catacgtcgc
      601 cgtcgatcaa cagcagcgcg tgtatgtggc cgatgaagtc ggccgacgcg tcctggtatt
      661 cgatgagaca ggcacctatc tgggcggctt cggcggattt ggtcttgata acaaaggttt
      721 tcagttgccc ggtggtatcg cggtcgataa agatggcttt atctatgtcg tcgacagcga
      781 ctcaagtcgc gtgttgaagt ttccgccgtt tcaggtgaag cagtgacgga aacggcccgt
      841 catttcgagc cgcgtttttt gcagtgagaa atccctgcga ggctagatag atcttgttca
      901 tgggaatgaa atttctcgcg gagtttacac tgagcaaagc gaatgtgttc gaaatgacag
      961 cgcgcaacaa cagatgtata attccaaatg tgatgcgcgc aaaataatct aaatcaatgc
     1021 atcaagggcg gcagtgcaac tgtcgcccta tctgcgtaat gtgatggagg tcaagtagtg
     1081 aagttacacg aatatcaatc gaagcaaatg tttgccaagt atggtattcc gatcccgaaa
     1141 ggcgaagtcg ccactcggcc ggaagacgcg cgcaggatcg ctgctgagtt ggggaagcgc
     1201 gtggtgatca aagcgcaggt gctggtcggc ggacgcggca aggccggcgg cgtgaagctg
     1261 gcaaataatc ccgacgaagc agaaaaagtt gccagccaaa ttttaggcat ggacatcaaa
     1321 ggcttgactg ttcacaaagt gctggtcgat gaagcggctg acatccaaca cgaaatttat
     1381 ctagccgtca cgctcgatcg agtgcagcgc cgcgtgatca tgatggcctc ggccgcaggc
     1441 ggcatggata ttgaagaagt ggcacgcacg gcgccggcga aaattatccg cgtggcgatc
     1501 gatccgtttc tgggcttgcg cgaatatcag acgcggcaga tcgcggccgc catcggcctt
     1561 gatcgatcgc tggtcaagga tttcaatacg atcgcgcaag gcttatatca atgttttatc
     1621 gatctagatg gcagcctggc cgagatcaat ccgctcatcg tcaatggggc gggcaagctg
     1681 caagccgtgg atggaaaaat cttgaccgac gataacgcgc tgtttcgtca cggtgatatg
     1741 gcggtgctgc gcgacgtgga tgaagaggcc ccatcggaaa cggaagctcg tcaaagagga
     1801 ctgtcgtata ttaaac
//
LOCUS       DASRTJ010000539         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72166,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000539 DASRTJ010000000
VERSION     DASRTJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72166"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFF70_10975"
     CDS             complement(<1..1565)
                     /locus_tag="VFF70_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45261.1"
                     /translation="MKTFRQSHLCVLSILIVLISAACTSTDLSVPPLPTPTSDKAVVN
                     LPPTAAPTASHPTEVAPQGPCRFSAAGSFTSGWYWLRDAAYTAIGQLDCRGLPTDRDL
                     TISLPTLVTNKADGGSGYSSPVKIIYLNPTSHLSHTVQVYLQNPSAEQSPANSKGVGY
                     PTTGYFVIPKAYIDTSGGLFVQIERFKPNTTHVAVNAQSLNFDRPRIADSFGQSKGIL
                     IADWYWLQDTARQAYGQWTFKGLSPTAPATLVFDLLVTNKTNGGSGYSTPIEITLINP
                     SNNSQKTLRHVSAQNLLFTQNPNNSNGIGYQTYGSIVLDNTFIDAHGNLIVRVARLSD
                     ATNHLAVNQNSVAIIQPEAAQDLATPLPGATKTSGLDGQPSGKYLFIQYWNKIDGTGT
                     LPALAIDFPDYRFDPRTGELSSFNPNRSITLASTAMGFIGHGTSRSGDAGTGAMSSLD
                     TIDRLPYSIDVAMFTGSVNVQAVPNMEETKPVGLKILAVAEDGTISIELDHQTIKLAP
                     GESWTRTVEAAIEG"
     gene            1822..>2053
                     /locus_tag="VFF70_10980"
     CDS             1822..>2053
                     /locus_tag="VFF70_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45262.1"
                     /translation="MRYLFLLINLSIILAACGSRITPTPIATLAPTATPRPTPTATPI
                     PNCLPISSTPTWIAPDTFDHYPDAIQNYLNNGG"
BASE COUNT          450 a          524 c          565 g          514 t
ORIGIN      
        1 ccttcaatcg cggcttccac tgtgcgcgtc cagctttctc cgggcgctaa cttgatcgtt
       61 tgatgatcaa gttcgatcga aatggttccg tcttcagcaa cggccaaaat cttaagcccg
      121 accggtttcg tctcttccat attaggcaca gcttgcacgt tgaccgatcc ggtgaacatc
      181 gccacatcga tcgagtaggg caaccgatcg atggtatcga ggctgctcat tgcgccggtt
      241 ccggcatcgc ccgatcgaga tgtgccgtga ccgatgaagc ccatagccgt tgaggccagc
      301 gtgatcgatc ggttggggtt gaaggacgac aactcaccgg ttctgggatc gaatcgataa
      361 tccggaaaat cgatcgcgag tgcgggcagt gtgccggtgc catcgatttt gttccaatat
      421 tgaatgaata aatatttgcc gcttggctga ccatcaagtc cggaagtctt tgtggcgcca
      481 ggtagtggtg tcgctaaatc ttgagcggcc tcgggttgaa tgatcgccac agaattttga
      541 ttgaccgcca aatgattggt agcatctgag agtcgcgcga cccgcacaat cagattaccg
      601 tgcgcatcga tgaaagtgtt atcgagcacg atcgagccat aagtctgata gccgatcccg
      661 tttgaattgt tcgggttctg agtgaacaac aggttttgcg cgctgacatg ccgcagcgtt
      721 ttttgtgaat tgtttgacgg attgatcaaa gtgatttcga tcggtgtgct gtaacccgat
      781 ccgccgttgg ttttattggt cacaagtaga tcgaatacca gagttgctgg tgctgtaggg
      841 ctgagtcctt tgaaagtcca ttgaccatat gcttgacggg ccgtgtcttg cagccaatac
      901 caatcggcga tcagaatgcc tttcgattga ccgaacgaat cggcaatgcg cggccgatcg
      961 aagttgagac tctgcgcatt gacggcgaca tgcgtggtat tcggcttgaa gcgttcgatc
     1021 tggacaaaca gtccgccact ggtatcgatg taggccttgg ggatgacaaa gtaacccgtc
     1081 gttggatagc caacgccttt actgtttgca ggcgattgct cagctgacgg attctgcaaa
     1141 taaacttgaa cggtatgtga tagatggctg gtcgggttga ggtaaatgat cttcaccggc
     1201 gagctgtagc ctgaaccgcc gtcggctttg ttggtcacaa gtgtcggcag actgatcgtc
     1261 aaatcccgat cggttgggag tccgcggcaa tccaattggc cgatggccgt gtaagcggca
     1321 tctcgcagcc aataccaacc gctggtgaaa cttccggccg cgctgaagcg acacggccct
     1381 tgcggggcaa cttcggttgg atggctcgct gtcggtgccg ccgttggcgg cagattgacg
     1441 accgctttgt cagaagtcgg tgtggggagt ggcggcacac tcaaatcagt tgaagtgcac
     1501 gccgcgctaa tgagcactat caggatcgat aggacgcaca ggtgcgattg tctgaacgtc
     1561 ttcatggata gcctccaggt ctgccctcct tggctatcgg ttggacattt gcatcccccg
     1621 atcgatgaga ttattcggcg tgttgattcc tattcgaatt tcagtataag gtcgtttgtc
     1681 gagaaagcaa taggcgcagg cgttgatttg caaaattgaa attgatcgat cgcgctagcg
     1741 ttatttgaaa aacccgccaa gtgtcccagc gcagtatggt acaatcgcgg cgcgttgtaa
     1801 acttcgcgaa tgatgatttc gatgcgttat ctatttctat tgatcaatct atcgattata
     1861 ctggccgcct gcggttcgcg gatcacaccc accccgatcg ccactcttgc tccgacggcc
     1921 actccgcgcc cgacgccaac ggcgacaccg atcccgaatt gtctgccgat cagctccacc
     1981 cccacttgga tcgcaccgga cacattcgat cattatcccg acgcgattca aaattatttg
     2041 aacaacggtg gac
//
LOCUS       DASRTJ010000540         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_34128,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000540 DASRTJ010000000
VERSION     DASRTJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34128"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="fabD"
                     /locus_tag="VFF70_10985"
     CDS             <1..862
                     /gene="fabD"
                     /locus_tag="VFF70_10985"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZY45263.1"
                     /translation="YPEARAIFDQADRILGVSLSRLCLDGPEDQLTDTVNAQPAILVT
                     SIATLRMIEKHAPHIKPAFVAGHSLGEFSALVATGSLSFEDAVRLVRERGRVMKAAGA
                     AQPGVMAAILNLGREVLDEVCKEASTATGQPVQVANDNSPGQIVISGDVTALDKAMEL
                     AKARGAKRAIKLAVSIAAHSPLMQPAARSFRKTLDATKFGSMTTPIIGNVYARPLDLE
                     DVRDELEAQLTSPVRWTESIQYLAEQGVTTCIEIGPKDVLTGLMRRIDPTLTAMSVGD
                     PTGIQLLLEK"
     gene            1031..1777
                     /gene="fabG"
                     /locus_tag="VFF70_10990"
     CDS             1031..1777
                     /gene="fabG"
                     /locus_tag="VFF70_10990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010023242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZY45264.1"
                     /translation="MALENKIAVVTGGSRGLGRGVALELAKRGARVVVNYNSNADAAN
                     EVVNLIKQAGSDAIAVQADVSKFEDAQKLIKAATEFGGRLDILVNNAGLTRDMLLAMM
                     PESDWDLVISTNLKSAYNCSKAAIRPMMRQKYGRIINMTSVAGLAGNGGQTNYSASKA
                     GLIGLTKSLAKEVGPRNITVNAVAPGYVLTDLTATLPKELIDGMLKLTPLSRLGNVED
                     VAYAVAFLASDEAAYITGHVLSVDGGMVMQ"
     gene            1855..2190
                     /locus_tag="VFF70_10995"
     CDS             1855..2190
                     /locus_tag="VFF70_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZY45265.1"
                     /translation="MTDEESHGTITIAPNVLNTIARLTTQNIEGVARLGASGKLLRAN
                     EGVAIEITDGRVKADIFIVVKPEVNLFDVGQQIQHNVARLIKDIVGMDVESVNVHVQD
                     VDYVSPKPG"
     gene            2227..2646
                     /gene="nusB"
                     /locus_tag="VFF70_11000"
     CDS             2227..2646
                     /gene="nusB"
                     /locus_tag="VFF70_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZY45266.1"
                     /translation="MKSRRRARSAALKALYELDTTTHQPGIVLSQRLAEESLTPDGEA
                     FAQQLVAGVWQHREQLDKMIQQYAPEWPIEQMAVIDRNLLRIAIFEFEIARITPLKVA
                     INEAIELAKAYGADSAPRFINGVLGSLAGPVESAKKI"
     gene            2648..3022
                     /gene="tatB"
                     /locus_tag="VFF70_11005"
     CDS             2648..3022
                     /gene="tatB"
                     /locus_tag="VFF70_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZY45267.1"
                     /translation="MEVFGIGPFELLLVVAIAFIVLGPERIPDVARSTGKAIRQIRQS
                     LQQLTTESGEKINIADDLAALRREVLGLQKDLTRATKDILMPSDQSPAPRNPVSISPP
                     STPESAPPREDDIIDMGAPPHT"
     gene            3019..3714
                     /gene="tatC"
                     /locus_tag="VFF70_11010"
     CDS             3019..3714
                     /gene="tatC"
                     /locus_tag="VFF70_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZY45268.1"
                     /translation="MSPESRMPFMQHLIELRNRLIKSFVALLVTTIVCAIFTPQILQF
                     LTAPYGNVLQTIDPTENVSIYFRVAIMSGAVLAMPVFVYELWSFLAPGLKSTEKRYAR
                     IVVPGATLLFLIGAGFAWGLMLPAAIGFLANFESEIFRADWRADAYIPFVTSLLFWIG
                     VSFETPLIIFFLAKLHVVTWRQLLKGWRFAIVIIAVVAAVITPTVDPFNMLLVMAPLI
                     LLYFLSVLLARLA"
     gene            3783..>4115
                     /locus_tag="VFF70_11015"
     CDS             3783..>4115
                     /locus_tag="VFF70_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZY45269.1"
                     /translation="MAKTKVLIMGAAGRDFHNFNTYFRDNDAYEVVAFTATQIPNIDG
                     RQYPPELAGKLYPKGIPIHPESELTNLITQHAVDQVVFAYSDVSHENVMHKASQALAA
                     GADFRLMGP"
BASE COUNT         1016 a         1042 c         1095 g          962 t
ORIGIN      
        1 atatccagaa gcgcgtgcca ttttcgatca agccgatcgg attttaggtg tgagcttatc
       61 tcgattgtgt ctggatggcc ccgaagatca attgacggat accgtcaacg cccagcccgc
      121 gatcttggtc acaagcattg caacattgcg tatgatcgag aagcatgccc cgcatatcaa
      181 gccagctttt gttgccggcc acagcctcgg cgaattcagc gcactggttg caactggatc
      241 actttcattt gaagacgcgg ttcgattagt tcgagagcgt ggccgagtga tgaaagcagc
      301 gggcgcagca cagcccggcg tgatggctgc catcctcaac ctcgggcgcg aagtattgga
      361 cgaggtttgt aaagaagcca gcacagcaac cggtcaaccc gttcaagttg ctaacgataa
      421 cagtcccggg cagatcgtga tcagcggcga tgtgactgct ttagacaaag caatggaatt
      481 agcgaaagcg cgcggcgcaa aacgcgcaat caaattggcg gtgagcatcg ctgcgcattc
      541 gccattgatg cagcctgcag ctcgatcgtt tcgcaagacc ctcgacgcga caaaattcgg
      601 atcgatgaca acgcccataa tcggcaatgt gtatgcccga ccgctcgatc tggaagatgt
      661 tcgcgacgaa cttgaagcac aattgacttc gcccgtgcgc tggactgaat cgatccagta
      721 tctggcggag caaggtgtga cgacctgcat cgaaattgga cccaaagatg tgttgactgg
      781 actgatgcgt cgcatcgatc caactctgac tgcaatgagc gtcggcgatc cgaccggcat
      841 tcaactgttg ctggagaaat aatttttcac gaaaaacact aaggaacgtt aggttagaat
      901 ttacgcttag ttgtcatttc gaacgctttt tgcgagaaat ctcgacgatc atgaacgagt
      961 cttgttcagc gggacagaga tttctcactt cgttcgaaat gacaagtgaa agccctgaag
     1021 gagcgtgatt atggcacttg aaaacaaaat tgcagtcgtg accggcggat cgcgcggcct
     1081 aggtcgaggc gttgcgctgg agctagcgaa gcgcggtgcg agagttgtgg tcaactacaa
     1141 cagcaacgcc gacgccgcaa atgaagttgt taatttgatc aagcaagctg gcagcgatgc
     1201 gatcgcagtg caggccgatg tgagcaagtt tgaggatgcg caaaaattga tcaaagccgc
     1261 gacggaattc ggcggtcgat tggatattct ggttaacaat gccggtctca cgcgcgatat
     1321 gctgctagca atgatgcctg aaagcgactg ggacctagtg atcagcacaa atctcaagag
     1381 cgcgtataat tgcagtaagg ccgcgatcag acccatgatg cggcagaagt acggtcggat
     1441 cattaacatg acttcggttg ccggactggc cggcaacggc gggcaaacga actattcggc
     1501 ctcgaaagcg ggcttgatcg gtttgacgaa gtcgctagcc aaagaagtcg gaccgcgcaa
     1561 catcactgtg aatgcggtcg caccgggtta tgtgttaacg gatctaaccg ctacgctgcc
     1621 aaaagagcta atcgatggga tgttgaaact cacaccctta agccgattag gtaatgtcga
     1681 agatgtggct tacgcggtgg cctttctcgc ttctgatgaa gcagcttata tcactggcca
     1741 tgtgttaagc gtcgatggtg gcatggtcat gcaatagatt gcaacccttg cgacggtttt
     1801 gcttctatag ataattcaat ccactaacct tcgcaagaat caaaggaaca agcgatgact
     1861 gacgaagaat cacacggcac cattacgatt gcccccaatg tattgaacac catcgcgcgt
     1921 ttgacgacgc agaacatcga aggtgtggcg cggttaggcg caagtggcaa attactacgc
     1981 gctaacgaag gcgtggcgat cgagattaca gatggccggg taaaagctga tatcttcatt
     2041 gttgtcaagc ccgaggtcaa cctgttcgat gttggccagc aaattcaaca caacgtcgcg
     2101 cgattgatca aagatattgt cggcatggat gtggaatcgg tcaatgtcca cgttcaggac
     2161 gtagactacg tcagccccaa accgggatag agcgatcgcc ttgacctcgt tcagcagcgc
     2221 atcagcatga aatccagacg acgtgcgcgc agcgcagcac tgaaagcact ttacgaatta
     2281 gataccacca ctcaccagcc gggcatagta ctgagtcaac ggttggcaga agaatcgctg
     2341 acgccagacg gcgaagcttt tgcgcaacaa ttagttgccg gcgtttggca acatcgcgaa
     2401 caactggata agatgattca gcagtacgcc cctgaatggc cgatcgaaca aatggccgtg
     2461 atcgatcgga atttgctgcg catcgccatc tttgaatttg agatcgcgcg catcacgcca
     2521 ctcaaggttg ccatcaacga agcgatcgag ttagctaaag catatggcgc agacagcgcg
     2581 ccgcgcttta tcaacggtgt gctaggttcg ctggccgggc cggtcgagtc cgcaaagaag
     2641 atttaacatg gaagtatttg gcattggtcc tttcgaatta ttgctggtcg tcgccattgc
     2701 attcatcgtg ttaggtccgg aaagaattcc agatgtagcc cgcagtaccg gcaaagccat
     2761 ccgacaaatt cgacaatcat tacagcagct cacgaccgaa tcgggtgaga aaatcaatat
     2821 cgctgatgat ttagcggcct tgcggcgcga agttcttggg ctgcaaaagg acttgacgcg
     2881 tgcgacaaaa gacatcctga tgcccagcga tcaatccccc gctcccagaa accctgtcag
     2941 catttcgcca ccgtccacac ctgagtcggc tccgccacgc gaagacgata tcattgatat
     3001 gggggcgccg ccccacacat gagccccgaa agccgaatgc cattcatgca gcatctgatc
     3061 gagttgcgca atcgcttgat caagtcgttc gttgcgttgt tggtgaccac gattgtctgc
     3121 gccatcttta cgcctcagat tttgcagttt ttgaccgccc catatggcaa tgtgctacag
     3181 acgatcgatc caaccgagaa tgtgtccatt tattttcgag tggcaatcat gtctggtgca
     3241 gtgttagcga tgccggtatt tgtctacgag ctctggtcat ttttggcgcc aggcttgaaa
     3301 tccactgaga aacgttatgc aagaattgtg gtgccgggtg ctacgctctt gttcttgatc
     3361 ggagcaggct ttgcctgggg gttgatgtta cccgcggcga tcgggtttct ggcgaatttc
     3421 gaatcggaaa ttttcagagc cgattggcgc gccgatgcgt atattccttt tgtcacgtct
     3481 ctgctctttt ggatcggcgt cagttttgag acgccgctga tcattttctt tctggcgaaa
     3541 ctgcatgtgg tcacatggcg gcagctgttg aagggttggc gtttcgcgat tgtcatcatt
     3601 gccgttgtcg ccgcagtgat cacacccacg gtcgatccgt tcaatatgct gctggtcatg
     3661 gcgcctctta tcctactgta tttcctgagc gtcttactgg cgcgtttagc ttaatctcgg
     3721 caattcacct cgataaaatc aatcgaggca aacagttaaa aaatttgttc aaggagctct
     3781 caatggcgaa gaccaaagtt ttaatcatgg gcgcagcggg acgcgacttt cacaacttca
     3841 acacctactt ccgcgataac gacgcctatg aagtcgtggc ctttaccgcg acacaaattc
     3901 ccaacatcga cggccgccag tatccgccgg agttggcggg caagctttac cccaagggca
     3961 ttccgatcca tcccgaatca gaattgacta acctgatcac tcagcatgct gtcgatcaag
     4021 ttgtctttgc ttattcggac gttagccatg aaaatgttat gcacaaagcc tcacaggcgc
     4081 tggcggccgg cgctgacttt cgtttaatgg gtcct
//
LOCUS       DASRTJ010000541         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_337297,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000541 DASRTJ010000000
VERSION     DASRTJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337297"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFF70_11020"
     CDS             complement(<1..549)
                     /locus_tag="VFF70_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZY45270.1"
                     /translation="MKISTTLYVTHRTQWRAWLVKNHKTAPEIWLVYYKKSSGKPRIP
                     YNDAVEEALCYGWIDSILKPIDQDCYAQRFSPRRKNSPLSEMNKERVRRLIKSKKMTR
                     FGLDSIRQHLDQAPAKSSRARQLKDFKLPDDILDALKADPIVWRNFKKFPKSYQHIRV
                     GWIDGVRKRPLEFQKRLRYFVKM"
     gene            complement(571..2238)
                     /locus_tag="VFF70_11025"
     CDS             complement(571..2238)
                     /locus_tag="VFF70_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZY45271.1"
                     /translation="MPQEEHSQRHRAEQLATLLLCLLILIACQAPQPTETPTPAPTIT
                     ATPIAPSTPTQQPEPTQAPRSITTMDNLIPKPVSIKSTGGTFMLTASTQIAIDPNSAE
                     VTQIGQYLADALKPATGYGLQVSTASSPLANGNILLTTVTGDPTLGEEGYELTITTDS
                     IKLIAYRSAGLFRGIQTLRQLLPASIENAAIQAGPWTVPTGTITDYPRFEWRGAMLDV
                     ARHFFSVDDVKRYIDLLAYTKINRLHLHLTDDQGWRIMINAWPKLATLGGSTEVGGGA
                     GGYYTQADYAEIVAYAQSRYMLVIPEIDLPGHTNAALASYAELNCDGIAPKLYTGTEV
                     GFSSLCGDKEITYQFLDDVIKELAAMTPGPYIHIGGDEAKATAPEEYKKFIERVQAIV
                     QAHGKQMIGWEEIASTQLLTTSIAQHWTSNLVRQAVQRGAKVIMSPATKSYLDMKYDA
                     STPLGQDWAAYIEVRDSYTWVPTTLVDGVTDSDILGVEAPLWSETLTTIKDIEFMAFP
                     RMLSIAEIGWSAASRDWDEYKVRLAAHGPRLTNMSVNFYRSPQVPWQ"
     gene            complement(2313..>2905)
                     /locus_tag="VFF70_11030"
     CDS             complement(2313..>2905)
                     /locus_tag="VFF70_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZY45272.1"
                     /translation="VAIIGSGVQARSHLAAMACVRSIRRVRVVSRSFDHAQRFVAEMR
                     PLYPFPVEAVKSVEEAVDHADLIVTATSSSEPILKREWISSGAHLNVVGASLPQAREV
                     DSATIAAASLFVDRRESTLNESGDYLFAVRDGAIGPDHIRAEIGEVLIGSKPGRSSID
                     EITLFKSLGLAIEDLAAAEYTYRQAQRSGIGTWVEF"
BASE COUNT          595 a          793 c          810 g          707 t
ORIGIN      
        1 catcttgacg aagtagcgca accgcttctg gaactcaagc gggcgcttgc gcactccatc
       61 gatccagccg acacggatgt gttgatacga tttggggaat ttcttgaagt ttcgccagac
      121 gattggatcg gctttcaagg cgtcgaggat gtcatcaggc aatttgaaat ctttgagttg
      181 ccgggcgcgg gacgacttcg ctggggcttg atcaagatgc tgccggatgc tgtccaatcc
      241 gaagcgcgtc atcttcttgc ttttgatcaa gcgccgtacc cgctctttgt tcatctcgga
      301 caatggactg ttcttgcggc gcggactgaa gcgttgagcg taacaatctt gatcgatcgg
      361 cttgaggatg ctatcgatcc agccgtagca caacgcttct tcgacggcgt cgttgtacgg
      421 gatgcgcggc ttaccactgc tcttcttata atagaccagc caaatctccg gcgcggtttt
      481 gtgatttttc actagccacg cgcgccattg cgttcgatgg gtcacgtaca gcgtggtgct
      541 gatcttcatc aggttaaact tttattttga ttattgccac ggcacttgcg gcgatcgata
      601 gaaattaaca ctcatgttgg tcagacgcgg accgtgagcg gccagacgga ctttgtattc
      661 atcccaatct cggctggccg ctgaccagcc gatctcggcg atcgacaaca tgcgcgggaa
      721 agccatgaac tcaatgtctt tgatcgtcgt caatgtctcc gaccatagcg gcgcttccac
      781 acccaggatg tcgctgtcgg tcacaccatc gaccagggta gtgggtaccc aagtgtaaga
      841 gtcccgcact tcgatataag ccgcccagtc ttgtcccaat ggcgttgacg catcgtattt
      901 catgtccaaa tacgatttgg tcgccgggga catgatcact tttgcgcccc gctgcacggc
      961 ctgtcgaacg agattgctgg tccagtgctg cgcgatcgat gtggtgagca gctgcgttga
     1021 ggcgatctct tcccagccga tcatttgctt gccatgcgct tgcacgatcg cttgtacgcg
     1081 ctcgatgaat ttcttatact cttcaggcgc agtggctttg gcctcgtcgc cgccgatatg
     1141 aatgtacggc ccgggcgtca tcgccgccag ctctttgatc acatcatcca gaaattgata
     1201 agttatttct ttatcaccac acaaggaact gaaaccgact tcggtgccgg tgtacaactt
     1261 cggcgcgatg ccatcgcagt tcagttcggc ataggacgcg agtgccgcat tggtatgccc
     1321 cggtagatcg atttcaggaa tcaccagcat gtagcggctt tgcgcgtaag ccacaatctc
     1381 cgcgtaatcg gcctgcgtat aatagccgcc ggcgccgccg cccacttcgg tgctgccgcc
     1441 aagcgtggcc agcttcggcc aggcgttgat catgatacgc caaccttgat cgtcggtcag
     1501 atgtaagtgc agtcggttga ttttggtgta cgccaggaga tcgatgtagc gcttgacatc
     1561 gtcgacgctg aaaaagtgcc gggcgacatc gagcattgcg ccgcgccact caaagcgcgg
     1621 atagtctgtg atcgttccgg tgggaacagt ccatgggccg gcttggatcg ccgcattctc
     1681 gatcgaggcg ggcagcaact gccgcagagt ttgtatgccg cgaaagagtc ccgccgaacg
     1741 atatgcgatc aatttgatcg aatcagtcgt gatcgttaat tcataaccct cttcacccag
     1801 cgttggatcg ccagtgacag ttgtgagcaa gatattcccg tttgccaatg ggcttgatgc
     1861 tgttgagact tgcaggccgt agcctgtggc tggcttcaat gcatccgcca gatattgacc
     1921 gatctgcgtg acttcagccg agttcggatc gatagcgatc tgggtgctgg ccgttaacat
     1981 gaacgtgccg ccggttgatt ttattgagac gggcttggga attagattat ccatagtcgt
     2041 gattgacctc ggtgcttgag ttggctcagg ttgttgggta ggtgttgagg gcgcgatcgg
     2101 cgtggcagtg atcgtcggtg ctggtgtcgg cgtttcggtt ggctgtggcg cttggcaagc
     2161 gatcaaaatc agcaggcaca gcaacaatgt ggcgagctgc tcggctcgat ggcgttgcga
     2221 atgttcttcc tggggcatac attttccctt gcatggttga cttcgacttt gagctgacaa
     2281 ttcccaaatg ttacagttga tcgcagatcg atctaaaact caacccaagt gccgatgccc
     2341 gatcgttgcg cttgccgata ggtgtattcg gccgcagcta aatcttcgat cgcgagcccc
     2401 agcgatttga agagcgtgat ctcatcgatc gacgatcggc ccggcttcga tccgatcagc
     2461 acttcgccga tctccgcgcg gatatgatcc ggcccgatcg cgccgtcgcg caccgcaaac
     2521 aaataatcgc cggattcatt caacgtcgat tcacgtcgat cgacaaacaa actcgctgcg
     2581 gctatcgtcg cggaatcgac ttcgcgcgcc tgcggcagac tcgcgccgac gacgttgaga
     2641 tgcgcgccgg atgaaatcca ttcgcgcttc aagatcggtt cgcttgatga agtggcagtc
     2701 acaatcaagt ctgcatgatc gacagcttcc tccacagatt ttactgcctc gacgggaaat
     2761 ggataaagcg gcctcatttc agccacgaag cgttgagcgt ggtcgaacga tcggctgacc
     2821 acacgcacgc gccggataga tcgaacgcac gccatcgccg cgagatgcga tcgggcttgc
     2881 acaccggaac cgatgatcgc tacat
//
LOCUS       DASRTJ010000542         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72385,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000542 DASRTJ010000000
VERSION     DASRTJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72385"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="mvk"
                     /locus_tag="VFF70_11035"
     CDS             complement(<1..886)
                     /gene="mvk"
                     /locus_tag="VFF70_11035"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HZY45273.1"
                     /translation="MITATAPGKIILFGEHAVVYGRPAIAAPVMQVQATVSIDRSDRR
                     GITIRSIDTNYTISIDRAGANDPLAAIVRLTIGHLGVPLPDCLISIRSTIPIASGLGS
                     GAAVSTAIVRALSQFVGQACLLPETVSALVYEVEKLHHGTPSGIDNSVIAYQCPVYFI
                     KGQPIQTFQVAAPFTVVIGNTGIASPTKIAVGDVRRAWAADRDRYEALFDQIGNIAQA
                     ARSAIESGSTDRLGPLMDRNQDLLRQIDVSSPELEKLIDAAKAAGAAGAKLVGGGRGG
                     NMIALVNESNRDQVVAALK"
     gene            complement(883..>1592)
                     /locus_tag="VFF70_11040"
     CDS             complement(883..>1592)
                     /locus_tag="VFF70_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZY45274.1"
                     /translation="LVDIEADRQHPKKKFRPLPSGQLPKSVAIIAVVLLPLIVMPIAF
                     RLDTIFGIILSIYALLNVAYSFKLKHVVIVDVIVVASGFVLRVGAGVPLVQVVRFSPW
                     MYVCIALLALFLGFSKRRQELMLLQNGATNSRAILEEYNLEFLDGMLNVIMASTILAY
                     SLYTFLAEGLPPNHSMMLTIPFVLYGIFRYLYLIHVRGEIAPPDEVLFKDRPMQIDVV
                     FWALSVVLILYLLPRTA"
BASE COUNT          378 a          428 c          465 g          321 t
ORIGIN      
        1 tcttcaaggc tgcgaccact tgatctcgat tcgattcatt taccagtgcg atcatatttc
       61 cgccgcgtcc accgccgaca agtttcgcgc ccgccgcgcc cgccgctttc gccgcatcga
      121 tcaacttttc cagttcggga ctcgatacat cgatctgcct cagcaagtct tgattgcgat
      181 ccatcagcgg accaagtcga tcggttgatc cgctttcgat ggccgatcgt gctgcttggg
      241 cgatgttgcc gatttgatcg aacaacgcct cgtagcgatc ccgatcggct gcccatgctc
      301 gacgcacatc gccgacggca atcttcgtcg ggctggcgat accggtgttg ccgatgacaa
      361 ccgtaaaggg agcagccact tgaaacgttt gaatcggctg tcccttgatg aaataaacgg
      421 gacattgata agcgatcaca ctgttgtcga tgccgctggg agtgccatga tgcaattttt
      481 caacttcata gacgagagcc gaaacagttt caggcaagag gcaagcttgc ccaacgaatt
      541 gcgacaacgc tcgaacgatt gcggttgaga ccgccgcacc gctgcccagt ccgctggcga
      601 tggggatggt cgatcggatc gagatcaaac aatcgggcag ggggacgccg agatgaccga
      661 tcgtcagccg aacaatcgcg gctaacggat cattagcgcc ggcgcgatcg atcgagatcg
      721 tgtagttcgt atcgatcgat cgaatggtga tgccgcgtcg atcagatcga tcgatcgata
      781 cagtcgcttg cacttgcatc accggcgcgg cgatggcggg tcgcccgtaa acgacggcat
      841 gttcgccgaa gagaatgatc ttgccgggag ccgtggcggt gatcatgcgg tgcgcggcag
      901 gagatagaga atcagcacga cgctcaaagc ccaaaacacg acatcgatct gcatcggtcg
      961 atctttgaac aacacttcat cgggcggcgc gatctcgccg cgcacgtgga tgaggtacaa
     1021 gtaacgaaag atgccataca acacaaaggg aatggtcaac atcatcgagt gattgggcgg
     1081 caggccttcg gcgagaaaag tgtagagcga gtaagccagg atggtgctgg ccatgatcac
     1141 atttaacatg ccgtcgagaa attcgagatt gtattcttcc aagatcgcgc gtgagttcgt
     1201 cgcgccgttt tgcaacagca tcaattcctg gcggcgtttg ctgaagccga ggaacaacgc
     1261 gagcagtgca atgcacacgt acatccacgg tgagaagcgc acgacttgta ccaggggcac
     1321 acccgcgccg acacgcagta caaacccgga cgcgacgacg atcacatcga cgatgacgac
     1381 gtgcttcaac ttgaatgagt aagccacgtt gagcagtgcg tagatcgaca ggatgatgcc
     1441 gaagatggtg tcgagccgga acgcgatcgg catgacgatc aacggcagca gcacgacggc
     1501 gatgatcgcg accgatttgg gcagttgacc ggagggtaga gggcgaaatt tctttttggg
     1561 atgctgtcga tcggcttcga tgtcgaccag at
//
LOCUS       DASRTJ010000543         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72550,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000543 DASRTJ010000000
VERSION     DASRTJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72550"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1366)
                     /locus_tag="VFF70_11045"
     CDS             complement(212..1366)
                     /locus_tag="VFF70_11045"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZY45275.1"
                     /translation="MTYAHIVGWGKFVPPKILTNNDLSKIVDTSDEWIVTRTGIRERR
                     IAGPKDSSATMGLAAAKDAIEMAGVRPTEIDLIICATATPEHYFPSTASLIQDALGAT
                     KAGAFDLSAGCSGFMYGLSMAAQIIQGGGHQVILVIGTETLSRIMNWQDRTTCVLFGD
                     GAGAMVLKASDNKGGLLSSLTRSDGSGGDLLIIPAGGSHLPATAETVQLGQHFIQMNG
                     REVFRFAARVIDRATREVVGKAGLTLDDIDLFIPHQANLRIIQAAARSLAVKEDRIFV
                     NLDRYGNTSSASIPIAICEAVEQGRLKPGDKIVMVGFGAGLTWAAAAVQWTGLEPIHQ
                     PNAVERSIDRIIYFGAAIRNRVVRVLRRIESALFGSPSPKTIDKGGKDKK"
     gene            complement(1442..>2365)
                     /gene="fabF"
                     /locus_tag="VFF70_11050"
     CDS             complement(1442..>2365)
                     /gene="fabF"
                     /locus_tag="VFF70_11050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZY45276.1"
                     /translation="GIGGISTIISQLEEMNAKGPERVSPFLVPMMLPDTASGQIAIQF
                     GLKGPNLCPVSACATGSHAIGEAAEWIRRGVADAVVTGSAEAAIIPIAMAGFNNMTAI
                     STRNDAPEKASRPFDKNRDGFVCGEGAGVLILEELEHAQARGAKIYAEVIGYGSSADA
                     YHITAPEESGAGAAQAMNMALAQAGLKPNQIDYINAHGTSTPLNDKGETAAIKRVFGE
                     AAYHVPISSTKSMTGHLLGAAGAVEGVIATKVITEGIIPPTINYETPDPNCDLDYTPN
                     TARRKQVNTIMSNSFGFGGHNAVVILSRYQN"
BASE COUNT          508 a          699 c          634 g          524 t
ORIGIN      
        1 gtaccattag gatctacggt tgtcatgctg agtgcagcga agcatctcgg ataattgtgc
       61 gagacccagc ccatgccttg agcccatcgc gctcaggtgc gccattaggc gtcgcctgcg
      121 ttcaggttga cagcgttcgc aaagacgcgg gcgttgagca gtacgcaatc gtaggcacac
      181 agagacgcct gttaaaggcg tctctgtgtg tctacttctt gtccttgcca cctttgtcga
      241 tagtcttcgg cgagggtgag ccgaagagcg ccgattcaat ccggcgcaag acgcgcacca
      301 cgcggttgcg aatcgccgcg ccaaaataga tgatgcgatc gatcgatcgc tcaaccgcat
      361 tcggctgatg gatcggctcg agaccagtcc actgcaccgc ggccgccgcc caggtcaagc
      421 ccgcgccaaa accaaccatc acaattttat cgcccggctt gaggcggccc tgttcgacgg
      481 cttcgcagat cgcaatcggg atgctggcac tggaagtgtt cccataccga tcgagattca
      541 cgaagattcg atcttctttc actgccagac tacgcgcggc cgcctgaata atccgcagat
      601 tagcttgatg cggaatgaac agatcgatat cgtccagcgt caatccggcc ttgccaacga
      661 cttcgcgcgt cgcccgatcg attacacgag ccgcaaagcg aaacacttcg cggccgttca
      721 tctgaatgaa atgctggccc agctgaacgg tctcggctgt cgcgggcaaa tgactgccgc
      781 ccgccggaat gatcaacaaa tcgccgcccg atccatctga tcgggtcaaa gacgataaca
      841 ggccgccttt gttgtccgac gctttcagca ccatcgcgcc cgcgccgtcg ccgaacaaca
      901 cacacgtcgt tcgatcttgc cagttcatga tgcggctcaa tgtttctgtg ccgattacga
      961 gaatgacttg atggccgccg ccttgaatga tctgcgctgc catcgacaag ccatacatga
     1021 aaccagagca gcccgcagag agatcgaacg cgccggcttt cgttgcgccc aatgcatctt
     1081 gaatgagaga ggccgtggag gggaaataat gttccggggt ggcggtggcg caaataatca
     1141 gatcgatctc ggtggggcgg acaccggcca tctcgatggc gtctttggcc gcggccagcc
     1201 ccatcgtggc cgatgaatct ttcgggccgg cgatgcgccg ctcgcggata ccggtacggg
     1261 tgacgatcca ttcgtcggag gtatcgacaa ttttagaaag atcgttattc gttaaaattt
     1321 tcggcgggac aaatttgccc cagcctacga tatgcgcata cgtcattgaa accttctttc
     1381 aaaaagtgaa gtgacacgca attgctggat cggtgattgc gtgtcacttc aaccccagag
     1441 tttagttctg ataacggctg agaatgacta cggcgttgtg accgccgaag ccgaacgagt
     1501 tggacatgat cgtattgact tgtttacgcc gggctgtatt gggcgtgtaa tccagatcgc
     1561 agtttggatc gggcgtctcg taattgatag tgggcggaat gataccctcg gtgatcactt
     1621 tcgtggcgat gaccccttcg accgcaccag ccgcgcctag caaatgcccg gtcatcgatt
     1681 tggttgagct gatcggcaca tgataagccg cttcgccaaa cacacgcttg atcgcagctg
     1741 tttcgccttt atcatttaat ggggtactgg taccgtgcgc attgatgtaa tcgatctgat
     1801 tcggcttcaa cccagcttgc gccagcgcca tattcattgc ctgagcagcg cccgcgcccg
     1861 attcttccgg tgcggtgata tgataagcat cggcgctcga tccgtagccg atcacttccg
     1921 cgtaaatctt cgcgccgcgc gcttgcgcgt gttccaattc ttccaagatt aaaacgcccg
     1981 cgccctcgcc gcacacaaag ccgtctcgat tcttatcgaa cggccgacta gctttctcag
     2041 gcgcatcatt gcgggtggag atcgccgtca tgttgttaaa gcccgccatc gcgatcggga
     2101 tgatagccgc ttcagcgctg cccgtcacca ccgcatcagc cacgccgcgt cgaatccatt
     2161 cagctgcttc accgatcgcg tgactgccgg tggcacacgc cgacaccgga cacaagttcg
     2221 ggcccttcaa gccaaattga attgcgatct gaccggacgc cgtatcaggt aacatcatcg
     2281 gcaccaagaa aggactgaca cgctcaggtc ctttcgcatt catctcttcc agttgactga
     2341 tgatcgtgct aatgccacca atgcc
//
LOCUS       DASRTJ010000544         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_337608,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000544 DASRTJ010000000
VERSION     DASRTJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337608"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /gene="obgE"
                     /locus_tag="VFF70_11055"
     CDS             <1..1413
                     /gene="obgE"
                     /locus_tag="VFF70_11055"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZY45277.1"
                     /translation="IDELPAECQTDRASAKPDRLSQPIENDLRTILFCGTINFDFIQS
                     IVSSMFFDTAQIEVKAGDGGDGAVAFRREKYVPFGGPSGGDGGHGGSVILYVDAKVNT
                     LSHFRHHHKFKADDGKDGRVKNQSGPAGADIWLAVPPGTIVRDATTHAVLGDLSEPKM
                     ELIVAKGGTPGKGNQHFATSTNQAPRMATNGLPGEIHTLELELKLIADVGLVGLPNAG
                     KSTFLAATTSARPKIADYPFTTLEPNLGVVDLDTETSLVTADIPGLIEGASLGKGLGI
                     EFLRHIERTRVLIHLLDGSQSDPMQDFHTINEELKAFGHGLIEKPQIVAYNKIDLPDA
                     QAQWPRIHKELKKAGFEAMAISAVTHAGTREVLYRAAQLLRDLPIPQPVVVDEVPVIR
                     TQDETSFVITRAPDASWHVTSKAVEKLALRHRFDSEESLMSFHKSITKLGVTDALRAA
                     GVKEGDTVWIGEDYELEWQD"
     gene            1515..>1991
                     /locus_tag="VFF70_11060"
     CDS             1515..>1991
                     /locus_tag="VFF70_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZY45278.1"
                     /translation="MIQAILFDLDGLMVDSEPHSIAAWQAVLAKRGATFDQAMIDRTF
                     GLRVDETSQLLKDTYHLRDSSADLAREKIEYQITYLNGNVKPMPGLIELLDLIDQRHL
                     KKAVASSSQRRYVDAVLRSIGLHDRFSVVIGGNEVSGGKPAPDVFLAAARALQVDPQ"
BASE COUNT          483 a          553 c          524 g          431 t
ORIGIN      
        1 atagatgagt tgcccgctga atgtcaaacc gatcgtgcat ccgcaaaacc cgatcgattg
       61 agccagccga tcgagaatga tcttcgaaca atcctgttct gtggtacgat taacttcgat
      121 ttcattcaat cgattgtgag cagcatgttt tttgatacgg cccaaattga agtaaaagcc
      181 ggtgatggcg gcgacggcgc cgtggccttt cgacgcgaaa aatacgtgcc tttcggcgga
      241 ccgtctggcg gcgatggcgg ccacggcggc agtgtcatct tatacgtcga tgccaaagtc
      301 aacaccctct cgcactttcg ccatcaccat aaatttaaag cagacgacgg caaagatggg
      361 cgcgttaaaa atcagagcgg tccggcgggc gccgacattt ggctggccgt gccgccgggc
      421 accatcgtgc gggatgccac gacgcatgcc gtgttgggcg atctgtccga gccgaagatg
      481 gaattgattg tcgccaaagg cggcacaccc ggcaaaggca atcaacattt cgccacctcg
      541 acgaatcaag caccgcgcat ggcgaccaac ggcctgcccg gcgaaatcca tacactcgaa
      601 ctggaattga aattgatcgc ggacgtgggt ctggtcggct tgccgaatgc aggtaagtcc
      661 actttcttag cggctaccac atctgcacgc ccgaagatcg ccgattatcc cttcaccacc
      721 ctcgaaccca atctgggcgt cgtcgatcta gataccgaga catcgctcgt taccgccgac
      781 attcccggct tgatcgaagg cgcgagtttg ggcaaaggac ttggcatcga atttctgcgc
      841 catatcgaac gcacccgcgt gttgattcat ctcctcgatg gatcacagtc cgatcccatg
      901 caggactttc acacgatcaa cgaagagttg aaagcctttg ggcatggcct gatcgaaaag
      961 ccgcagatcg tagcttacaa caagatcgat ctaccagatg cccaagcgca gtggccgcgc
     1021 atccataaag aattgaaaaa ggccggcttt gaagccatgg cgatctccgc cgtgacacac
     1081 gcgggcacgc gcgaagtatt gtatcgtgcc gcacaattgc tgcgcgatct gcccattcca
     1141 caaccggtcg tcgtcgacga ggtgcccgtc attcgcactc aagatgaaac atctttcgtc
     1201 atcacccgcg caccggatgc cagttggcat gtgaccagca aggcggtgga aaaacttgcc
     1261 ttgcgtcatc gctttgattc ggaagaatcg ctaatgtcct ttcataaaag catcaccaaa
     1321 cttggcgtca ctgatgcgct gcgcgctgcg ggtgtcaaag aaggcgacac agtctggatc
     1381 ggcgaagact acgaacttga atggcaagac taaaccacgg ctgtcacgga tgacaaggat
     1441 tttgttttaa tctgtgacag ccatgtcatc cgtggctaat tttttcgtgt cgatacgcgg
     1501 atcaaattac caaaatgatt caagccatcc tctttgatct cgacggcctg atggtcgata
     1561 gcgagccaca ctcgatcgca gcttggcaag ccgtactcgc caagcgcggc gcaacattcg
     1621 atcaagcgat gatcgatcga actttcggtc tgcgcgtgga cgaaacctca caactcctta
     1681 aagatactta tcatttacgg gacagcagcg ccgatcttgc ccgtgagaag atcgaatatc
     1741 aaatcacgta tctgaatggc aatgtgaaac cgatgcccgg cttgatcgaa ttgctggact
     1801 tgatcgatca gcgccacttg aagaaagcgg tggcgagcag cagtcaacgc cgttacgtcg
     1861 atgctgtgtt gcgatcgatc ggtttacacg atcgatttag cgtggtgatc ggcggcaatg
     1921 aagtctcagg tggcaagcct gcacccgatg tcttcctcgc cgcggcacgc gcgttgcaag
     1981 ttgatccaca g
//
LOCUS       DASRTJ010000545         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_72627,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000545 DASRTJ010000000
VERSION     DASRTJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72627"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFF70_11065"
     CDS             complement(<1..627)
                     /locus_tag="VFF70_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY45279.1"
                     /translation="MSDTILYAIADNVATITFNRPEVRNAFNDQMADELQAALKNAER
                     DEAVRCIVITGAGQGFCAGQDLAAIRDRSDEVSFRDHLKKTYNPIVSKLRSIEKPVVA
                     AINGAAAGAGWGIALACDIRYASEAAKFRLAFIGIGLAPDSGTSYFLPRMVGLGKALE
                     LAYTNDLVDANTALSLGLVNKVVPADQLMPATLEFAKKLAQGPTRGMGL"
     gene            complement(697..>2085)
                     /locus_tag="VFF70_11070"
     CDS             complement(697..>2085)
                     /locus_tag="VFF70_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HZY45280.1"
                     /translation="PSGDALLDRQARLMRDSFQSQQMSPDMIKQIVALETDITAEFNG
                     FRAELNGQSMTDNQLKKILKESNDEVERRAAWEASKQIGAQVVDRVRQTVQLRNRAAR
                     EMGFANYYAMSLHLQELNEDTLFALFDRLEKLTRPLFTAYKTDLDRQLADRFHLLIDQ
                     LRPWHYADPFFQETPAMPGLNLDQYYADKDIVALAQQFYQKIGMPVDDILGRSDLYER
                     EGKYQHAYCIDIDRQGDVRTMDNVQPNARWMGTLLHELGHAVYDKYHDRSLPFLVREP
                     AHTLSTEAIAMLMGRFSENGAWLRHYLGLSPSETVKFETKLKAFQSAGLLIFTRWDLT
                     VCHFEREMYRNPDQDLNKLWWDLVEKFQMLKRPEGRNQPDWASKIHIACYPAYYQNYM
                     LGEMTASQLLEFINTEVLGGHAEKFVESPEVGKYLVNKYFKLGSRFDWNGLLKESTGE
                     ELRPEYFAKHLQ"
BASE COUNT          459 a          591 c          548 g          487 t
ORIGIN      
        1 cagacccata ccgcgcgttg gaccttgcgc caacttcttg gcaaattcca gtgttgccgg
       61 catcaattga tcggccggca cgactttgtt gaccaagcct agcgacaagg cggtgttcgc
      121 atcgacgaga tcatttgtat aggccaactc taaggctttg cccaagccaa ccattcgcgg
      181 cagaaaatat gatgtgcccg aatccggcgc caggccaatg ccgataaacg ccaagcggaa
      241 cttggccgcc tctgaggcgt atcgaatgtc gcaggcgagc gcgatacccc agcccgcgcc
      301 cgctgccgcg ccgttgatcg ccgcaacgac cggcttctcg atcgagcgca gcttcgacac
      361 gatcgggttg taggtttttt ttaggtgatc gcgaaaggaa acttcatcac ttcgatcgcg
      421 aatcgcggct aaatcctggc ccgcgcagaa accttgacct gcacccgtga tgacgatgca
      481 gcgcacggct tcgtcgcgtt cggcgttctt cagcgcggct tgcagctcat ccgccatttg
      541 atcgttgaag gcgttgcgca cctcgggtcg attgaaggtg atggtcgcga cgttgtcggc
      601 aatggcatag agaatcgtgt cagacaatac agcctccctc gatgaaaacg tgatacgtgc
      661 tgcgtgaggt tcatatcacg cagcacatat cacgcattac tgtaaatgct ttgcgaaata
      721 ttctggccgc agctcttccc cggtcgattc cttgagcagg ccgttccaat cgaagcgcga
      781 gccgagtttg aaatatttgt tgaccagata cttgccgact tcaggactct cgacaaattt
      841 ctcagcgtgg ccgcccagca cttcggtgtt gatgaactcc agcagttgcg aagcggtcat
      901 ttcgcccagc atatagttct gataataagc cgggtagcag gcgatgtgaa tcttcgatgc
      961 ccaatcgggt tggttacggc cctccggccg cttgagcatc tggaacttct cgaccaaatc
     1021 ccaccacaac ttattcaaat cttgatcggg gttgcgatac atctcgcgct cgaaatggca
     1081 gaccgtcaga tcccagcgtg tgaagatcaa taacccggca ctctggaacg cttttaactt
     1141 cgtttcgaat ttaacggtct cgctcggaga taaaccaagg taatgccgca gccatgcacc
     1201 attttcgcta aaacggccca tcagcatcgc aatcgcttcg gtcgataagg tatgcgcggg
     1261 ttcgcgcacc aagaacggca gggagcgatc gtgatatttg tcgtagactg catggcccag
     1321 ttcgtgcagc agggtgccca tccagcgcgc attcggctgc acattatcca tcgtccgcac
     1381 atcgccttgg cgatcgatgt cgatgcaata cgcatgttga tacttgccct cgcgctcata
     1441 caaatcgctg cgcccgagaa tatcatcgac gggcatgccg atcttctgat aaaactgctg
     1501 cgccaaagcg acgatgtctt tatcggcgta gtattgatcg agattgaggc cgggcattgc
     1561 cggcgtctct tgaaagaaag gatcggcgta gtgccacggc cgtagctgat cgatcaggag
     1621 gtgaaaccga tcggctagct ggcgatccag atcggttttg taagcggtga acaacggccg
     1681 cgtgagtttt tccaatcgat cgaacaaagc gaacaatgta tcttcattca attcctgtaa
     1741 gtgcaagctc atggcataat aattggcgaa acccatttca cgcgcggcgc gattacgcag
     1801 ctgcacggtt tgcctgaccc gatcgaccac ctgcgccccg atctgtttgc tagcttccca
     1861 ggcggcgcgc cgctcgacct catcgttgga ttctttcaga atctttttca actgattgtc
     1921 tgtcatcgat tggccgttga gttcagcgcg aaagccgttg aactcggccg tgatgtcggt
     1981 ctccagcgca acgatctgtt tgatcatatc cggcgacatc tgctggcttt gaaagctatc
     2041 gcgcattaac cgcgcctgcc gatcgagcag cgcatcaccg ctagg
//
LOCUS       DASRTJ010000546         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_337646,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000546 DASRTJ010000000
VERSION     DASRTJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337646"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFF70_11075"
     CDS             complement(<1..434)
                     /locus_tag="VFF70_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45281.1"
                     /translation="MRGSLKLMAKSELHPRSLMLHAVGDEVTSLGPNVAMKAHTHPFD
                     LAFSLWAPSIDSDALAKGEHIFPFEFRLPAILPPTFNGDLTQIVYRIEAKVDLPLQPD
                     LHIEQSFSVQVAPIVAAEKPLRATSHLTNGLTLELQLYAVSF"
     gene            complement(601..1134)
                     /locus_tag="VFF70_11080"
     CDS             complement(601..1134)
                     /locus_tag="VFF70_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZY45282.1"
                     /translation="MDTYKSIISLRSVRHFDRSKPIDDQALHRILQAGRMSGSSKNSQ
                     PWWFIVIQDQATLEALSNCGDFAQHLAGATLAIAIVFDPKFYRGEFDSGRTAQNMLLA
                     AWNDGIGSCIASMHREDDTKKVLKVPGEYRVQHILSFGYPQLREQSTSAVKKSGRKSM
                     DEMVFQEKWGKHVTSNE"
     gene            complement(1183..2166)
                     /locus_tag="VFF70_11085"
     CDS             complement(1183..2166)
                     /locus_tag="VFF70_11085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45283.1"
                     /translation="MNTQLIAQGFRLREYLPSDIPAFVAILNRTFPDNPRDVEKEKYM
                     ESIYPPDNPRLRYAVENSAGRVIGVGTCLRPFWMNMTDTYSMWITIDPAWRRRGIGQS
                     MLGKLEVYAQQQGAVKLRTDCREDSADSIEFLKTCGFNNYGLRFESQIDLTQIAESKF
                     GAVIDRATADGYEFTTLAAERTINPEADRCLYELDELTSEDVPLPGGARWESSFENFK
                     HMNLEGPESDVNAIGIAKYRGQFVGLTIVWFGKDKPAYTAMTGVRREHRGHGLALALK
                     LLSFRAIKARGYTAVRTTNDTANPAIMRLNEKLGYQKLPGNMLWEKVISHE"
     gene            complement(2192..3151)
                     /locus_tag="VFF70_11090"
     CDS             complement(2192..3151)
                     /locus_tag="VFF70_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45284.1"
                     /translation="MTQGIRFREYRLSDIPAFVEIANRVYPDDPNTVENEEYFERIYP
                     PDNPRLRYAVENSAGQLIGGGTCILPFWQYAPGVYMMWITLDPDWRRRGIGRAMLDKF
                     ETFARQQGAERLRTDCREDMAYSIEFLKAMGFTNFGLRYESELDLKSFDESKFGNAVD
                     RAIAAGYEFTTLTTERTINPEADRLLYDVDRVTRDDVPMPGGTRSRSSYEDFRRINLE
                     SPESDPSAIFIAKHDDQYVGHTMVWFGKDKPAYTAMTGVQREHRGHNLALALKLLSFR
                     LMKERGYSATRTTNDTANPPILRLNEKLGYQKIPGNSLWEKVL"
     gene            complement(3187..4290)
                     /locus_tag="VFF70_11095"
     CDS             complement(3187..4290)
                     /locus_tag="VFF70_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HZY45285.1"
                     /translation="MKIKSALVTKINQPIQIETLDLEAPHAGEVLVRIAACGVCHSDY
                     HLISGATKHPLPVVPGHEGAGIVESVGPNVTRVKPGDHVVLNWAPNCGHCFYCLRGKP
                     NLCDTFIGPIWDGVMLDGTPRLSWRGDPVYHFSALAAFAEYTVVPQETCVVIRDDVPL
                     KVAALVGCAVTTGVGAVLYTADVHAGDRVAIFGCGGVGLSILLGAKLAGAQQIIAIDR
                     VPKKLELAQQLGATDVIDASQVDAIDQVRRLTNGRGADVSFEAVGVPQLQADCVEAAR
                     PGGKAVLVGLSAHGSVTPLSGAKIARQEKTILGSYYGSADTARDFPFLLDLYQAGKLD
                     LDALVSETYELPQINEAFNKMLSGEIGRGVVVF"
     gene            complement(4287..4961)
                     /locus_tag="VFF70_11100"
     CDS             complement(4287..4961)
                     /locus_tag="VFF70_11100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZY45286.1"
                     /translation="MSDEISTHLAKQVDEYIEHLLIPQDAALEQGLKDAEAAGLPPIS
                     VSANEGKLLYLIAKIANAKRVLEIGTLGGYSTTWLARALPYDGMLITLELDQKHAEVA
                     RKNLDRAGVGDRVEIRIGTAIYSLQQMLDQHEESFDMIFIDANKDSYVEYLNLSLQLA
                     HTGTVILADNLIRNGRALEENPPDENARGAKAYNQAMAANPRLDSIILPIIRTNLDGI
                     SISIVK"
     gene            complement(5058..6134)
                     /locus_tag="VFF70_11105"
     CDS             complement(5058..6134)
                     /locus_tag="VFF70_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZY45287.1"
                     /translation="MSERTIVVLEGDQTGQELLEESLRVLDPKVIRVPLNFQRFDLSL
                     ENRRATNNKVVLDSAEALKVWGLGIKAATITPEKAGDAGSPNAILREAIDGKVILRVG
                     RRIPNVRPVGGVYAPIAVVRMAVDDAYGAKEWREGSGLDEVAYRTEKITRGTCHAVAE
                     FAFRYAKKTGAKVFGGPKFTVSPIYEGMFKEELDAAAKRHPDVRYEPQLIDATYALLL
                     VKAGEALVVPALNRDGDCLSDLVLQMFGSIAGSESMILSFDQDFNVKTAVAEAPHGTA
                     PGLFKKNVANPLAMILAGAALLTYIGSTEADRASRAIYESAFEAIYDGVATADLGHQV
                     GTTEFTDEVIKRVEKKLEVWGTLG"
     gene            complement(6212..7438)
                     /gene="coaBC"
                     /locus_tag="VFF70_11110"
     CDS             complement(6212..7438)
                     /gene="coaBC"
                     /locus_tag="VFF70_11110"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZY45288.1"
                     /translation="MDTIPLLQNKKFVLGICGGIASFKIVDLASKLTQAGSQVDCVLT
                     ESAAKFVSPLTFASVTGSPALIDADLWRADLHVPHVSLGESADLFVIAPATANTIAKL
                     AHGQADNLLTVTALAARCPIVVVPAMDGAMWSNAATQENIATLKSRGLHVLSPATGRM
                     ASGLIGEGRMVEASEIFGFIRVLLGANGKLSGRKIVVTAGGTREAIDPVRYIGNRSSG
                     KQGFAMAQAAIDRGAQVTLIVGASSLDMPYGAERIDVESTEQMRAEVLHACQNADALI
                     MAAAVADYQTEQVADQKIKKTQETVEFNVKLIRTPDILLAVKAQKESIGQPKITIGFA
                     AETQNLIENAKAKLSNKGLDLIVANDVAAEDAGFAVDTNRVTVIGRGGGVEQYPLMSK
                     PQVAERVLDRLIELLG"
     gene            complement(7610..8377)
                     /locus_tag="VFF70_11115"
     CDS             complement(7610..8377)
                     /locus_tag="VFF70_11115"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZY45289.1"
                     /translation="MLLTLDISNTTIKAGIFKGEQLLADWRFATEREKLTDEYAMLLL
                     SLFQASGIPASEIDGVSMSCVVPPLRSVFDDLAKKYLHRTPLIVGPGIKTGVKLAVDN
                     PREVGADRVVNSLATYRLYGGPSIAIAFGTATVFDCVSAQGEYLGGAIAPGVAVALES
                     LTRSAAQLFQVELVRPPKAIGKSTVTTMQSGVVLGFAGLVEGLVKRLKAELDPKAKVI
                     ATGGLADVIAPATDVIDVVDHELTLKGLRLIYELNAA"
     gene            complement(8565..>9070)
                     /locus_tag="VFF70_11120"
     CDS             complement(8565..>9070)
                     /locus_tag="VFF70_11120"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZY45290.1"
                     /translation="VATVVAKLFNITGADRAYFGQKDAQQVIVIKRMVADLAMPIQIV
                     VCPTLREADGLAMSSRNVYLDAEHRQAAPVLYRALSAAKDAFDRGERNGDALRSIMRT
                     VIEKEPLAKIEYVSAADATTLQEADSVDESHGLLLSMAVRVGKPRLIDNFLYDNGQWL
                     MRKFING"
BASE COUNT         2019 a         2601 c         2296 g         2154 t
ORIGIN      
        1 taaaacgaga ctgcgtacag ttgaagttcc agtgtcaagc cgttggtcaa atgcgaggtg
       61 gcgcgcaatg gtttttccgc agcgacgatt ggcgcgacct gcacgctgaa tgactgctcg
      121 atgtgcagat cgggctgcag cggcagatcg actttggctt caatccgata aacgatctga
      181 gtcagatcgc cattgaaagt gggcggcagg atcgcaggta gccggaattc aaatgggaaa
      241 atgtgttcgc ccttagctaa cgcatcgcta tcgatcgacg gtgcccacaa actgaaggct
      301 aaatcaaaag gatgggtgtg cgctttcatg gcgacgttgg ggccgagcga agtcacttca
      361 tcgcccaccg catgcagcat caggctgcgc ggatgcagct cggacttggc catcaatttc
      421 aagctgccgc gcacggtttc gccggggcga taagtgtgat tcgccgattc gagttgaagg
      481 gaaagttgag cgggtatagt gacctcctgg ccccgttagt ttatcacaat gtcgccctga
      541 gttaaacgtg aagagtgaaa aataatgagt aacgagttat gagtaattgc cagacgcgga
      601 ttactcatta ctcgttacat gttttcccca cttctcctgg aacaccatct cgtccatcga
      661 cttgcggcca ctctttttaa cagcgctcgt actttgctct cgcagctggg gataaccgaa
      721 cgagaggatg tgctgcactc gatattcgcc gggcaccttc aaaaccttct tggtatcatc
      781 ctcgcggtgc atcgaagcga tacacgaacc gatgccgtca ttccatgcgg ctagcagcat
      841 gttctgtgcc gtgcggcctg aatcgaactc gccgcgatag aatttcggat cgaacacgat
      901 ggcgatggcg agtgttgcac ccgccagatg ctgtgcaaag tcgccgcagt ttgataaagc
      961 ctcgagtgtg gcttgatcct gaatcacgat gaaccaccac ggctgactgt tcttgctgct
     1021 gccgctcatg cggccggctt gcaaaatgcg gtgcagcgct tgatcgtcga tcggtttgga
     1081 tcgatcgaag tgacggacgg agcggagaga gatgattgat ttataagtgt ccatggaatg
     1141 atcaccgctt aatgtgcaat tcttaatgaa gtaacgtcat tcttactcat gactgattac
     1201 tttttcccac aacatattgc ccggcagctt ttgatacccc aacttttcat tcaaacgcat
     1261 gatggctgga ttggcagtgt cgttggtggt gcgaactgcg gtatagccgc gcgctttgat
     1321 tgcgcggaag gataagagtt tcaacgctaa tgctaaaccg tgtccgcgat gttcacgccg
     1381 gacgccggtc atggcggtgt aagcgggctt gtctttgccg aaccacacaa tcgtcaggcc
     1441 gacaaattgt ccgcgatatt tggcgatgcc aatggcattg acatctgact ctgggccttc
     1501 caaattcatg tgcttaaaat tttcgaaaga ggattcccag cgcgcgccgc cgggcagtgg
     1561 cacatcttcg ctggtgagtt catcgagttc atacaagcac cgatcggctt caggattgat
     1621 cgtgcgctcg gcggctaagg tggtgaattc gtagccgtcc gctgtggctc gatcgatcac
     1681 agcgccgaat ttcgattcag cgatctgggt aagatcgatt tgcgattcaa aacgcaagcc
     1741 gtagttgtta aacccgcatg tcttcagaaa ctcgatcgag tccgcgctgt cctcgcggca
     1801 gtctgtgcgt agtttaactg cgccttgttg ctgcgcataa acttccaact tgcccaacat
     1861 cgattgcccg ataccgcgcc gccgccaagc cggatcgatc gtgatccaca ttgagtaggt
     1921 gtctgtcata ttcatccaaa acgggcgcag gcatgtgcct acaccaatca ctcgccccgc
     1981 actgttttca accgcgtaac gcaaacgcgg attgtccggt ggataaatgc tctccatgta
     2041 tttttctttt tcaacatctc tggggttgtc aggaaaagtg cgattgagta tggccacaaa
     2101 agccggaata tcactgggta gatactctcg aagtcggaag ccttgtgcaa tgagttgagt
     2161 gttcatgagg cgctcctgct gttgatcaag gttacagcac tttttcccac aatgaattgc
     2221 ctggtatttt ttgatagccc aatttttcat tcagccgcaa aatcggtgga ttggctgtat
     2281 cgttggtggt acgggtagca ctgtaaccac gctctttcat caatcggaag gaaagtagtt
     2341 tcaaggccaa ggctagatta tgtccgcgat gctcgcgctg cacgccggtc atcgctgtgt
     2401 aagccggttt gtctttgcca aaccacacca tcgtgtggcc gacgtattgg tcgtcatgct
     2461 tggcaataaa aattgccgaa ggatcagact ctgggctttc cagattaatg cggcgaaagt
     2521 cctcgtagct cgatcgactg cgcgtgccgc ccggcatcgg cacatcgtcg cgcgttaccc
     2581 gatcgacatc gtataacaac cgatcggctt ccggattgat ggtacgttcg gtggtcagtg
     2641 ttgtgaattc gtaaccggcg gcaatcgctc gatcgaccgc attgccgaat ttcgattcat
     2701 caaacgattt caaatcaagt tctgattcat agcgcaggcc gaagttggtg aagcccatgg
     2761 ctttcagaaa ttcgatcgag taagccatat cttcgcggca gtcggtacgc aatcgctccg
     2821 cgccttgttg gcgggcaaaa gtttcgaatt tgtccagcat cgcccggccg atgccgcgcc
     2881 gccgccaatc gggatcgagc gtgatccaca tcatatacac accgggcgca tattgccaga
     2941 acggcaggat gcacgtacca ccgccgatca actggcctgc gctgttttca accgcgtaac
     3001 gcagacgcgg attgtccggc ggatagatgc gctcgaagta ttcttcgttt tcaacggtgt
     3061 tgggatcgtc tggataaacg cgattcgcaa tttcaacaaa ggccggaatg tcgctgagtc
     3121 gatattcgcg aaagcgaata ccttgagtca tgagttgctc attcatcttc attcacctgc
     3181 ttcattttaa aacacaacaa ctccacgccc gatctcgccg ctcagcattt tattaaaagc
     3241 ctcattgatt tgaggcagtt cgtacgtttc tgaaacgagc gcatcgagat cgagtttgcc
     3301 tgcttgatac agatcgagca agaatggaaa atcgcgcgcc gtgtcagccg atccgtaata
     3361 cgaaccgagg atcgtttttt cctgccgagc gatcttcgcg cccgacagcg gcgtgaccga
     3421 gccgtgcgcc gacaaaccga ccagcacggc tttgccgccc ggtcgggcag cttcgacaca
     3481 atcggcttgc agctgcggca caccgactgc ttcgaaggac acatccgcgc cgcgtccgtt
     3541 cgtcaagcgg cggacttgat cgatcgcatc gacttggctc gcatcgatca catccgtcgc
     3601 gcccaattgt tgggcgagtt caagtttctt cggcacccga tcgatcgcga tgatctgctg
     3661 cgcgcccgcc agttttgcgc cgagtaaaat gctcagcccc acgccgccgc aaccgaagat
     3721 cgccactcga tcacccgcgt gtacatcggc ggtatataac accgcgccaa cgccggtcgt
     3781 caccgcgcag ccaaccagcg ccgcaacttt cagtggcaca tcgtctcgaa tgaccacgca
     3841 agtttcctgc ggcacgacag tatattcagc aaacgcggcc agtgccgaga agtgataaac
     3901 cggatcgccg cgccaactca atcgaggtgt gccatccagc atgacgccgt cccagatcgg
     3961 accaatgaag gtgtcgcaca aatttggttt gccacgcaga caataaaagc agtgaccgca
     4021 gttcggcgcc caattaagca cgacatgatc gcccggcttc acgcgtgtga cattcgggcc
     4081 gacggattcg acgatgcccg cgccttcgtg accgggcacc acgggcagcg gatgtttagt
     4141 cgcgccgctg atcaaatgat aatcgctgtg gcacacgccg caggctgcga tgcgcaccag
     4201 cacttcaccc gcgtgcggcg cttccagatc gagcgtctcg atttgaatcg gttgattgat
     4261 cttggtgacg agcgcagatt tgattttcat ttgacgatcg agatcgatat gccgtctaag
     4321 tttgtgcgga tgatcggcag aatgatcgaa tcgagtctcg gattggcggc catggcttga
     4381 ttgtaggctt tcgccccgcg cgcattctca tcgggcgggt tttcttccaa cgcgcgcccg
     4441 ttgcgaataa gattgtctgc caagatcacc gtgccggtat gagcgagttg cagcgataga
     4501 ttcaaatatt caacgtagct atctttattt gcgtcgatga agatcatgtc gaacgactct
     4561 tcatgttgat cgagcatctg ctgcaacgaa tagatcgctg tgccgatccg aatctccact
     4621 cgatcgccga cacccgctcg atcgagattc tttcgcgcca cttcggcgtg cttttgatcg
     4681 agttcgagcg tgatcaacat gccatcatat ggtaaagcac gtgccagcca agtggtgctg
     4741 tatccgccca gcgtgccgat ctctaaaaca cgtttggcat tggcgatctt cgcgatcaaa
     4801 tacagtaact tgccttcgtt ggccgaaacg ctgatcggtg gtaagccagc cgcttcggca
     4861 tccttcaagc cctgctcaag cgccgcatcc tgcggaatta acaaatgctc gatgtattca
     4921 tcgacttgct tggctaagtg cgtgctaatt tcgtctgaca tagtctctcc ttaatgaccg
     4981 cggaccgatg accgatgacc gccgctgatc acacttcaat cagactcatc tgcggtcttc
     5041 tgtctgctgt cctcggtcta gcctaaagtg ccccacactt ccaacttctt ctcgacgcgc
     5101 ttgattactt cgtccgtgaa ttctgtcgtt ccgacctgat gaccgagatc cgccgtggcg
     5161 acaccgtcat agatcgcttc gaacgccgat tcgtaaatcg cacgcgacgc tcgatcagct
     5221 tccgtcgatc cgatgtaggt tagcaacgcc gccccagcca gaatcatcgc caacggattt
     5281 gccacgttct tcttgaacaa gcccggcgca gtgccatgcg gcgcttcggc cacagcggtc
     5341 ttcacattga agtcttgatc gaaactcaaa atcatcgact cgctgccggc gatcgatccg
     5401 aacatctgca aaaccagatc gctcaagcaa tcgccatcgc gattcagcgc cggcacaacc
     5461 agcgcctcac cggctttgac cagtaaaagc gcgtacgtcg catcgatcag ctgcggctca
     5521 tagcgcacat cgggatggcg ctttgctgcc gcatcgagtt cttctttgaa catgccttca
     5581 tagatcggac tgacggtaaa cttcggccca ccaaaaactt tcgcgccggt ctttttggca
     5641 tatctaaatg caaattcggc tacggcatga catgtgccgc gtgtgatctt ctccgtgcga
     5701 taggccactt catccaggcc ggatccctcg cgccactctt tcgcgccgta ggcatcatcg
     5761 acggccatgc gcacgacggc gatcggcgcg tacacaccgc caacaggccg cacattcgga
     5821 atgcggcggc ccacgcgcaa aatcactttg ccgtcgatcg cttcgcgcaa aattgcattc
     5881 ggactgccgg catcgcctgc tttctccggc gtgatcgtcg cggccttgat gcccagtccc
     5941 caaactttca acgcttcagc cgaatccagc acgactttgt tattcgtcgc gcgtcgattc
     6001 tccaacgaca aatcgaaacg ttgaaaattc agcggcacgc ggatcacttt gggatccagc
     6061 acgcgcagcg attcttccaa caactcttga ccggtctggt caccttccaa aacaacaatt
     6121 gttcgctcag acatcatggc tcctcaaaaa taattcagcg gcgatcattc gatcgccgct
     6181 gctattttac tccatttgtt gttaaatccg tttaaccgag caattcaatc aatcgatcca
     6241 gcactcgctc agcgacttgt ggtttgctca tcaacggata ttgttcgacg ccgccacccc
     6301 ggccaatcac tgtcactcga tttgtgtcga cggcgaagcc ggcatcttcg gccgcgacgt
     6361 cattggcaac gatcagatcg agacccttat tcgatagttt ggccttggca ttttcgatca
     6421 aattctgcgt ctcggcggca aatccgattg tgatcttcgg ctgcccgatc gattctttct
     6481 gcgctttaac ggccagcaaa atatccggtg tgcggatcaa cttgacgttg aactcgaccg
     6541 tctcttgggt cttcttgatt ttctgatcgg cgacttgctc cgtctggtaa tctgcaacag
     6601 ccgcggccat gatcaaagca tccgcgtttt gacaggcgtg caagacttca gcgcgcattt
     6661 gctccgtcga ttccacatcg atccgttccg caccgtacgg catgtccagt gatgatgctc
     6721 caacgatcaa cgtcacctgt gccccgcgat cgatcgccgc ctgcgccatc gcaaagccct
     6781 gcttgccgct cgatcgattg ccgatatacc gcaccggatc gatcgcttca cgcgtcccgc
     6841 ccgccgtgac gacaattttt ctgccgctta atttcccatt tgcgcctaac agcacgcgaa
     6901 tgaacccgaa tatttctgac gcttcaacca tccgcccttc gccgatcaga ccggatgcca
     6961 ttcggccagt tgccgggcta agcacatgca agccgcgtga tttcaacgtt gcaatatttt
     7021 cctgagtcgc cgcgttcgac cacatcgcgc catccatcgc aggcacaacc acgatcggac
     7081 atcgcgcggc cagtgccgtg accgtcagca gattatccgc ttgcccatgc gccagtttcg
     7141 caatcgtgtt cgcggtcgca ggcgcaatga caaataaatc cgccgattca cccagcgaca
     7201 catgcggtac gtgcaaatcc gctcgccaca aatcagcatc gatcagagca gggctgcctg
     7261 tgacggacgc aaacgtcaac ggcgatacaa attttgccgc cgattcagtc aacacacaat
     7321 cgacttgtga tccggcctgc gtcaacttgc tggcaagatc gacgattttg aatgaagcaa
     7381 ttccgccaca gattcctaga acgaattttt tattttggag aagaggaata gtgtccatag
     7441 tgaaatgacc aatgacaaat gacaaagttt ttgattgtca ttccgagcga agcgaggagt
     7501 ctctatgccg atcaacagcg atcgctgcag tggtagagat tcctcgtcgt ggcacttcct
     7561 tagaggctgt gccatctaag gactgcgcac ctcggaatga cagcgactat cacgcagcat
     7621 tcaactcgta tatcaaccgc aagcctttca gcgtcaactc atgatccacc acatcgatca
     7681 catccgtcgc aggtgcgatc acatccgcga ggccgcccgt cgcaatcact ttcgcttttg
     7741 gatcgagttc ggccttcaaa cgcttgacta agccttcgac aagtccggca aagcccagca
     7801 ccactcccga ttgcattgtt gtcacagtac tcttgccgat cgccttgggc gggcgcacga
     7861 gttctacttg aaagagctgc gcggccgatc gggttaatga ttccaacgcg acggcgacgc
     7921 cgggagcaat cgcgccgccg agatattcgc cctgtgccga aacacaatcg aacacagttg
     7981 ctgtgccgaa cgcaatcgcg atcgatggtc cgccatacaa tcgatacgtc gccagcgaat
     8041 tgaccacgcg atccgcgccg acttcgcgcg gattatcgac ggcgagtttg acaccagtct
     8101 tgatgcccgg tccgacgatc agcggtgtgc gatgcaaata tttttttgcc agatcatcaa
     8161 ataccgatcg cagtggcggc acgacacacg acatcgacac gccgtcgatc tcgctggctg
     8221 gaatgccgct agcttgaaac aggctgagta gcagcatcgc atattcatcg gtgagtttct
     8281 cgcgctccgt cgcaaagcgc caatcggcca gcagttgttc gcccttgaag atgccagctt
     8341 tgatggtggt gttgctgatg tccagtgtta ggagcattgt tgatcccaga tgaagtcgtt
     8401 aatgtgtatt gagtgaaaat gatgcgtgat acgtgacgag tgaagcgtct ttgcgagaag
     8461 catcctgagc ggagcgcgta gcgcgaagtc gaaggacgcg acgaagcaat ctcataatcg
     8521 atcgggagat tgccacgacg catgcggcgg ctcgcaatga cacttcatcc atttataaat
     8581 tttctcatca accactgacc attgtcatat aagaaattat caatcagtct cggtttgcca
     8641 acgcgtacag ccatcgatag caagaggccg tgtgattcat caactgaatc tgcttcttgt
     8701 agcgtcgtcg catctgccgc cgaaacatat tcgatcttcg ccagcggctc tttctcaatc
     8761 acggtcctca tgatcgatcg caacgcatca ccgtttcgtt cacctcgatc gaatgcgtct
     8821 ttcgccgcac tcaaggctcg atacaaaacc ggtgcggctt ggcgatgttc cgcatcgaga
     8881 tacacattgc ggctcgacat tgccagccca tcggcttcgc gtaaagtcgg acacacgacg
     8941 atctggatcg gcattgccag atcggcgacc atgcgcttga tgactatcac ttgctgcgcg
     9001 tccttctgcc cgaagtacgc tcgatccgcg ccggtgatgt tgaacaactt tgccaccacc
     9061 gttgccacac
//
LOCUS       DASRTJ010000547         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_262171,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000547 DASRTJ010000000
VERSION     DASRTJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262171"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFF70_11125"
     CDS             complement(<1..765)
                     /locus_tag="VFF70_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HZY45291.1"
                     /translation="MNPINDIFIIIANFLRSLLGVGGLHLADWFVELIMAVLRAALLG
                     TFALLTFMVLTWIERKVVARIQDRLGPNVAGPWGILLPIADGIKMLVKEDTVPAGADK
                     VVFSLAPLVAAVAAMLVYAVIPFGPDTGHGALAGADLNIGIFYILAVGAVSLIAILMA
                     GWSSNNKYALLGAFRAVAELLSYEVPQVLSITVVVMLAGTMQMTQIVQAQTVPFIFPL
                     FATAIIFFISGVAESGRTPFDLVEAESEIVAGYHIEY"
     gene            complement(840..1199)
                     /locus_tag="VFF70_11130"
     CDS             complement(840..1199)
                     /locus_tag="VFF70_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZY45292.1"
                     /translation="MLQDFAFVGIFVIVGTGLVGLPLILNWILAPKKPNKIKSETYEC
                     GMETVGETWVQFKAQYYIYALVFAIFDIETVFLFPWAVAYKQFGTIFPLLEMILFVLI
                     LAGGLIYVWRKGALEWA"
     gene            1555..2583
                     /locus_tag="VFF70_11135"
     CDS             1555..2583
                     /locus_tag="VFF70_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HZY45293.1"
                     /translation="MPFHFITDPTEWNAALATLPSRHVLQSWEWGQFKSRWGWSPRYL
                     LYEESGQTRGAALILRRTVGRFLNILYVPKGPALDYSDAALADRILGELAQIAQQDRS
                     IFIKIDPDLAAADRTILLDRGWRSSRDQIQFRNTVLIDLTPNDAQLLAAMKQKTRYNI
                     RLAQKKGVIIREGTLSDLELLYAMYAETSRRDGFLIRPLDYYRDAWGSFIQSGLAQPL
                     IAEVDGQAVAGLILFHFADRAWYMYGMSRNVHRDLMPNHLLQWQAMQWAKSRGCTVYD
                     LWGAPDELIESDSMWGVYKFKEALGGKFAEHVGAYDFVASRFWYWVYSVAMPKVLEVM
                     RSRHQTQA"
     gene            2750..3110
                     /locus_tag="VFF70_11140"
                     /pseudo
     CDS             2750..3110
                     /locus_tag="VFF70_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017219902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
BASE COUNT          770 a          859 c          797 g          684 t
ORIGIN      
        1 atattcgatg tgatatccgg ccacaatttc cgattcggct tcgaccagat cgaacggcgt
       61 gcgtcctgac tcggcgacgc cgctgatgaa aaagatgatc gccgtggcaa acagcgggaa
      121 gataaacggc acggtctgcg cctgaacgat ctgcgtcatc tgcatggtgc ctgccagcat
      181 gaccacgacg gtgatactca acacttgcgg aacttcatag gagagcaatt cggccacggc
      241 gcgaaatgca ccgagcaatg catatttatt gttgctgctc caacccgcca tcaaaatcgc
      301 gatcagcgac accgcgccaa ccgcaagaat gtagaaaata ccaatgttca gatcagcacc
      361 ggccagcgcg ccatgaccgg tatcgggacc gaatggaatc acggcataca ccagcatcgc
      421 ggcaacggcg gcgacaagcg gcgccaggct aaacaccact ttatccgcac ctgccggcac
      481 ggtatcttct tttaccagca ttttgatgcc atccgcgatc gggagcagaa taccccacgg
      541 accggctacg ttgggaccca atcgatcttg aatgcgcgcc accactttgc gttcgatcca
      601 ggtcaagacc atgaaggtga gcagggcaaa cgtgcccagc aaagccgccc gtaacacggc
      661 cataatcagc tcgacaaacc aatcggctaa atgcaaaccg cccacgccca acaggctacg
      721 caggaaattg gcaatgataa tgaagatgtc gttgattgga ttcatactcg atgcctcaca
      781 acaaacgccc cactcccgag tcatcggggg cggggcggtc tgggctcatc gcccgaaatc
      841 taggcccact caagggctcc tttgcgccac acgtaaatca atccgccggc caagattagc
      901 acgaacaaga tcatctccaa cagcgggaag atagtgccga attgtttata cgccacggcc
      961 cacggaaaca agaagaccgt ttcaatatcg aagatggcga agaccagcgc gtaaatgtaa
     1021 tactgcgctt taaactgcac ccatgtctcg ccgacagtct ccatgccgca ttcgtaagtt
     1081 tcacttttga ttttattggg tttcttggga gcgagaatcc aattcaggat taacggcagt
     1141 ccgactaaac cggtgccgac gataacgaag atgcccacaa atgcaaaatc ttgaagcacg
     1201 cttgcctccc gacgatcgag tgcaggtgaa gtcactcaca aattgcaacg gttatagtat
     1261 cacacacccg attgttctgc aaagcccgac ggtacgcact gcgcgcttta gggttgtgag
     1321 gtctttcgac ctcacaacca ttaccgcatg tgtcgaactt ctggctcgct gcgtcgcggg
     1381 cgtattctac caccaacttt ttgcctgcgc aaagcgcttc aatcaaaatc gattatactt
     1441 gttcaaccgt cattgcgacc ctaagtgtag cgaaggggaa gcaatctcat tgattagcag
     1501 ctgggattgc ttcgtcgtgc ttggcactcc tcgcaatgac gcatcggatt cacgatgccc
     1561 tttcacttca tcacagatcc aactgaatgg aacgccgcgt tagccacact gccctcgaga
     1621 cacgtcttgc aatcatggga gtgggggcag ttcaaaagcc gctggggctg gtcaccgcgt
     1681 tacctgttat atgaggagag tggccaaaca agaggggccg ccctcatttt gcgccgcacg
     1741 gtcggtcgat ttctcaacat cctgtacgtc cccaaaggcc cggcgctgga ttactcagat
     1801 gccgcattgg ccgatcggat tctgggcgaa ctagcccaga tcgcccaaca agatcgatcg
     1861 atcttcatca agatcgatcc ggatctcgcc gcagccgatc gaacgatctt gctcgatcgg
     1921 ggctggcgat cctcgcgcga tcaaattcag ttccgcaaca cggtattgat cgatctgact
     1981 ccaaacgatg ctcagttgct ggcagccatg aaacagaaga cgcgttacaa catccgattg
     2041 gcgcagaaga agggcgtgat cattcgtgaa ggcacattga gcgatcttga attgctctat
     2101 gccatgtatg ctgagacttc ccggcgcgat ggcttcttga ttcgcccgct cgattattat
     2161 cgtgacgcgt ggggcagctt tatccaatcg ggcttagcac aaccgttgat cgccgaagtg
     2221 gatgggcaag cggtcgctgg tttgatcctc tttcacttcg ccgatcgagc gtggtatatg
     2281 tacggcatgt cgcgcaacgt gcatcgcgat ctcatgccga atcatttgtt gcaatggcag
     2341 gcgatgcagt gggccaaatc gcgcggttgc acggtgtacg atttatgggg cgcgccagat
     2401 gagttgatcg aatctgattc gatgtggggt gtgtacaaat tcaaagaagc tctcggcgga
     2461 aaattcgccg agcacgtcgg cgcgtatgat ttcgtcgcgt cacgattttg gtattgggtc
     2521 tattcagtcg ccatgccgaa agtgctggag gtgatgagaa gcaggcatca gacccaagct
     2581 taaagcaagc tccaagttgt tttacgatgt ctgggtagac gttccgtcac acccgcgaag
     2641 gagggtgtcc agatttcaaa ctggattccc gctttcgcgg gaatgacact gcgtgacaca
     2701 ccatcaacca aactggaatg tgcgctaacg caagttcagg agaaatgtta tgctaggccc
     2761 ttccccgcgc caaccttacc ccatgccgct cgatccgaag atgtgttttc tgaaaatatt
     2821 attcggaatc ccaacatcat catcggcgat tacacctatt actacgattt cgacgagccg
     2881 gaaaagttcg agcggaatgt cctgtatcat ttcccgttca atcacgataa actgatcatc
     2941 ggcaagttct gttcgatcgc gtccgatgtg aagttcatca tgaacggcgg caatcatcgc
     3001 accgattgga tcaccaacta tccgttcccc gtttttggcg aaggctggga aacagcgatg
     3061 ccggatgcat ggccgaacaa aggcgacacc gaaatcggca acgatgtgtg
//
LOCUS       DASRTJ010000548         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_355397,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000548 DASRTJ010000000
VERSION     DASRTJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355397"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VFF70_11145"
     CDS             <1..1728
                     /locus_tag="VFF70_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45294.1"
                     /translation="RFCVSLIQKLFPLLFIAVLTIWGVYALQIAPVNAGGLPVPAPGF
                     WREWQSAQYYLNQPWPNYLFGQTSITGWWYYFPIAFVLKTPLPILILLVIALARSIIK
                     RSWRRDLIYLLPSLLLFISLLFSTNNLGYRYLLPMLPLFFIYISGIFAVSLSRFASIS
                     LFRSFALSPFHLVILSLLAWQIIGALHIYPYFLTFFNEIAGGPDRGRYALSDSNIDWG
                     QDLVGLKNYVDQHHIDQIKLSYFGIAHPTAYDLKVEALLPVRTALNDQGAWWLKKYTP
                     IDPAPGTYAISVANLMGGIWIDPAQYRYFRDRTPDTIIGNSIYIYMIGSRGNPIDLSL
                     AGLQIDQIDPETYARFNTNDVRPRWFDATSSVIAAPNQTWLAINDNQPIAPEFSAFFA
                     GIQSETQTRTIDDHQPYHLYHFDLGQRIQQVARQTNSTAIWSPQIYPNSSATNIVTLP
                     VRFGETAQLIGFNLAKVSEPSQGLTLITYWQAGDHVIAPLQLFLHAIGPAGSIEAQED
                     RLDASAFGWHSGDLIAQINHLALPGRSGSYWIEIGLYNQDSGARLPIIVDGREVDHRL
                     LLRQISTGR"
     gene            1728..2330
                     /locus_tag="VFF70_11150"
     CDS             1728..2330
                     /locus_tag="VFF70_11150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45295.1"
                     /translation="MPTGNGMMIDTGLQSAPRQVTHNFHWVILIVILLGAFLRWADFG
                     SMKAMLYYDEAYYGANAVSLIQQPRLTPFFPGNFGRESGWMYLLNPFIATFGAQPFAL
                     RLAAGMVGVLTLAAVYALAKELLGRRGAGWSTAALVILYWHVHMNHIGWRANLLTLGG
                     SLAFASLLRAYHLNRFSHWLFTGVLIGLLTYTYFLHVLGS"
     gene            2330..3625
                     /locus_tag="VFF70_11155"
     CDS             2330..3625
                     /locus_tag="VFF70_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45296.1"
                     /translation="MAILFLMAFVLLDAQRRKGSLLAAVIATVLSIPMLVYLFTYPEQ
                     SLSRINDVAALSVGSIGQNIELWLKAWFQQGDLALMPNIPGRPILDPFMGILFLGGLI
                     GVLVFIRRRWHKVLFLGLAIAALIPSLLSQDAPHFLRANGLTVVIAVIVGAGALLIER
                     VIAKRVGQTVVMIVLSGLLLTSGLITFQDFQRWLVDPRVAIYMEEYVNQPIDYLKHTT
                     PSDLPVYGLPHSSDHPLIRFRGSDLAPRPLRAFDTFFCQVTTDVPADYVTITAYEPNY
                     SEVLSRWADVQVITASLPLSGGGLQLDDVYGEINPSFRPPPTPQKDRGVLPFYTIYQA
                     TPHSDWVSGQDQLTAIFGNNVRLRSILPIPSTLHAGDDISIQLGLTALHALDKDYNIF
                     IHLYGSAPPSEGGKVWRVTDEPACAAYPASDWQTHEIVV"
     gene            3865..>4769
                     /locus_tag="VFF70_11160"
     CDS             3865..>4769
                     /locus_tag="VFF70_11160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45297.1"
                     /translation="MFAQMIFASPTQSAAFDEGYTITYGYAYLRTGDARLSRGQNPPL
                     TDVLIALPLLLRNDIKFPIDQPAWTKGDIFGFTDEFLWKANADPQRIILLARLPEMLL
                     AMLLACVIYAFTRSLFDNYAALAALFLCAFDPNILAHGHISGTDLGVTFFMFSSVWLL
                     TSSLKRTSARRAIIAGLLAGAALSSKYSAVWLAPIVLLIVIFLPGLRHQWLARFKLLL
                     IFGVLTFGVIWATFGFSIGPIAPSDPVLPAPDYWSSLEVVRNRVELSTPAFMLGQVSA
                     TGFPLYYPFVFLIKTPLPTLILLAVG"
BASE COUNT          974 a         1228 c         1173 g         1394 t
ORIGIN      
        1 cgtttctgtg tctctttgat tcaaaaacta tttccacttc ttttcatcgc ggtgctgact
       61 atttggggcg tgtatgcttt gcaaattgcg ccagtaaatg ccggtggtct gcctgtgcct
      121 gcacccggct tctggcgcga atggcaatcg gcacagtatt acctgaatca accctggccc
      181 aattatctct ttggtcaaac atcgattacc ggttggtggt attactttcc gatcgcgttc
      241 gtcttgaaaa cgccgttgcc gattttgatc ctgctggtca tcgccttagc ccgatcgatc
      301 atcaagcgct catggcgacg cgatctcatc tatctcctgc cgtcactttt gctcttcatc
      361 agtttactct tcagcactaa caacctcggc tatcgatact tgctgccaat gctgccgctt
      421 ttctttatct acatctccgg cattttcgcc gtctctctat ctcgctttgc ctctatctct
      481 ctctttcgct ctttcgcctt gtcacccttt cacctagtca tcttgtcact cttggcttgg
      541 caaatcattg gtgcgctgca catttatcct tactttctaa ctttctttaa cgagatcgcg
      601 ggtggtcctg atcgaggccg ttatgctttg tccgattcca acatcgattg gggacaagat
      661 ctggttggct tgaagaacta cgtcgatcag catcacatcg atcagattaa actgagttac
      721 ttcggcattg cacatcccac cgcgtacgat ttgaaagttg aagcgttgtt acccgttcgc
      781 acggcgctga atgatcaagg cgcttggtgg ctgaagaaat acacgccgat cgatcctgcg
      841 cccggcacct acgcgattag cgtggcgaat ctcatgggcg gcatctggat cgatccggcc
      901 caataccgat actttcgcga tcgaacgccg gatacaatca tcggcaactc aatctacatc
      961 tacatgatcg gctcacgcgg caacccgatt gatctatcac tggccggact gcagatcgat
     1021 cagatcgatc ctgagactta cgcgcgcttc aataccaatg acgttcgtcc gcgctggttc
     1081 gatgcgactt cctcggtaat cgctgcacct aatcaaacgt ggttggcgat aaacgataat
     1141 cagcccatcg caccagaatt ctccgccttt tttgctggga ttcaatctga aacacaaacc
     1201 cggaccattg atgatcatca accgtatcat ctctaccact ttgatttagg gcaacgcatt
     1261 caacaagttg cacgtcaaac caactcgaca gcgatctgga gtccgcagat ttatcctaac
     1321 tcatcggcta cgaatattgt gactttgcct gtaagatttg gtgagactgc gcagctgatc
     1381 ggattcaacc ttgcgaaggt ttcagaacct tcgcaaggtt tgactttgat cacttactgg
     1441 caggctggcg atcacgtcat cgcaccgctt caattattct tgcacgccat tggaccagct
     1501 ggatcgatcg aggcacagga agatcgactc gatgcttcgg cctttggctg gcactcaggc
     1561 gatctcatcg cgcagatcaa tcatttagcg ttgccaggtc gatcgggatc atattggatc
     1621 gaaatcgggc tgtataatca ggattccggc gcacgcctgc cgattatcgt tgatgggcgt
     1681 gaagttgatc accggctgct attacggcaa atatccacgg gccgctaatg ccaacaggta
     1741 atggaatgat gatcgatact ggattgcagt ctgctccccg ccaagtcacc cacaactttc
     1801 attgggtgat tctcattgtt attttgttag gcgcatttct gcgttgggct gactttggct
     1861 cgatgaaagc catgctgtat tatgacgaag cttactatgg cgcgaatgca gtctcgttga
     1921 ttcaacagcc acgtttgacg ccgttcttcc caggaaattt tggccgggag agcggttgga
     1981 tgtatttgct taacccgttc atcgcaactt ttggagcgca gccatttgcg ctacgattgg
     2041 cggctggtat ggtcggcgtt ttgactttgg ccgccgtgta tgctcttgct aaagaactat
     2101 tggggcggcg aggtgcgggt tggtcgaccg ctgctctggt cattctgtat tggcatgtcc
     2161 atatgaatca tatcggctgg cgagctaatt tattgacgct tggtggctct ttggcgtttg
     2221 cgagtctgtt gagagcttat cacctcaatc gcttctcgca ttggttattc actggcgtgc
     2281 tgattgggtt gttgacttac acttattttc tgcacgtact tggatcgtag tggcgatatt
     2341 gttcttgatg gcatttgtac tattggatgc ccaacggcgg aaaggcagcc tactcgctgc
     2401 tgtgattgcc acagtcctat ccatacctat gttagtctat ctttttacct atccggaaca
     2461 atcacttagc aggatcaatg atgtcgctgc tctgagtgtg ggtagcatcg ggcagaatat
     2521 tgagttgtgg ctgaaggctt ggtttcagca gggcgatctg gcgctgatgc ccaatattcc
     2581 aggtcggccc atccttgatc cattcatggg catattgttt ttaggcggac ttataggcgt
     2641 gctggtattt attcgacgcc gttggcacaa ggttttattt ctgggcttgg caatcgctgc
     2701 gttgataccg agtttactca gtcaagatgc tcctcatttt ttgcgtgcga atgggttgac
     2761 tgttgttatc gctgtcattg tcggtgcagg cgcgttgttg attgagcggg taatagccaa
     2821 gcgcgtcggt caaaccgtag tcatgatcgt tctgtctgga ctacttctga cttccgggct
     2881 gattaccttc caagatttcc agcggtggtt ggtcgatcct cgagttgcga tctatatgga
     2941 ggagtatgtc aatcagccga ttgattactt aaaacacacc acgccttcgg atctgccagt
     3001 gtatgggttg ccccattcga gcgatcatcc attgatcaga tttcgtggct cagatcttgc
     3061 accacgtccc ttgcgagcct ttgacacttt tttctgtcag gtgacaaccg atgtgccggc
     3121 agattacgtg acgatcaccg cttatgagcc caactattca gaggtattat cgcgctgggc
     3181 ggatgtgcag gtcatcacgg ctagtttgcc cttgtcgggc ggaggcttgc agctcgatga
     3241 tgtctatggt gagatcaatc cctcgtttcg tccaccgcct acgcctcaga aagatcgagg
     3301 cgttttaccc ttttatacaa tctatcaagc tacgcctcac tccgattggg tatcaggtca
     3361 agatcagctg acagccatct tcggcaataa tgtgcgtttg cgttcgatcc tgccaatccc
     3421 gtcgaccctt catgccggtg atgatatctc aatccaactg gggctaacgg cactgcacgc
     3481 gctcgacaag gattacaata tcttcattca tttatatgga tcggctccac caagtgaagg
     3541 tggcaaggtg tggcgtgtta ccgatgaacc tgcgtgtgct gcttatccgg cgtcggactg
     3601 gcagactcac gagatagtcg tataggattt tactctatcg atcccaactg atacgccacc
     3661 tggaaaatat atcgtcgcga ctggcattta tgatcgatcg acaaacaatc gtttgccact
     3721 cacttggccg ataacagaaa accaggattt ctttggcttg caagaaataa acattatcaa
     3781 gtaacttggg tgtcttttta agtgacaagt aaacggctga acgcgaagtt tcgatggttg
     3841 attggcatca ctcttttgat gatcatgttc gcccaaatga tctttgcttc acctacgcag
     3901 tccgccgcgt tcgatgaagg ttacacgatc acttacggtt acgcttactt gcgcaccggc
     3961 gatgcgcgcc tgagtcgcgg acagaatcca cccttgacgg atgtattgat tgcattgccg
     4021 ctgctattgc gtaatgacat caagtttccg atcgatcaac cggcctggac gaaaggcgac
     4081 attttcggct tcacagatga atttttgtgg aaagccaatg ccgatccgca gcgcatcatt
     4141 ttgttggcgc gtttgccaga gatgttgctg gcgatgttgt tggcttgcgt catctatgcc
     4201 ttcacgcgat ccctgttcga caattacgct gcgctggccg cattgttctt atgcgcgttc
     4261 gatccgaaca tcttggcgca cggccacatc agcggcaccg atcttggcgt aacgttcttc
     4321 atgttcagtt cggtttggct gttgacgtca tcgctcaaac gcaccagcgc gcgccgggcg
     4381 atcatcgcag ggttgttagc gggtgctgca ttgtcgagca aatattctgc ggtatggctt
     4441 gcgccgattg tgttgctgat tgtaattttc ctgcctggcc ttcgacatca atggctcgcg
     4501 cgtttcaagc ttctgctgat ttttggtgtg ttgacctttg gcgtcatctg ggcaaccttc
     4561 ggtttttcga tcggcccgat tgcgcccagt gatcccgttc tccctgcgcc cgattactgg
     4621 tcatcactcg aagtggtacg taatcgtgtt gaattaagca cgccagcctt catgctcggc
     4681 caagtttcgg cgacgggctt tccgctgtac tatccatttg tgtttctgat caaaacaccg
     4741 ctgccgacgt tgattctact tgcggtcgg
//
LOCUS       DASRTJ010000549         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_337745,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000549 DASRTJ010000000
VERSION     DASRTJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337745"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..705
                     /locus_tag="VFF70_11165"
     CDS             205..705
                     /locus_tag="VFF70_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HZY45298.1"
                     /translation="MTEQDQFEPVIIGFTCNWCSYRAADLAGTARVKYPPNVRLIRLM
                     CSGRLDPTFVMKALASGADGVLITGCHPGECHYIDQNYKALRRYHLLKRVLAQLGIEP
                     KRVKLVWASAAEGVKLAHEITMLVEEIRALGPLHWGAANGHEVHDHLPMIEEEMEVKE
                     TLEVAA"
     gene            702..1709
                     /locus_tag="VFF70_11170"
     CDS             702..1709
                     /locus_tag="VFF70_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZY45299.1"
                     /translation="MTTNGTKPKFAMYWASGCGGCEIAVLNIGEKILDVDANFDVVFW
                     PVAMDAKYKDVEALEDGSILLCLFNGGMRTHENEEMAKLLRRKSKILVAFGSCATEGC
                     IPGLANFSSPQEIFETAYNTISTNNPGHIIPLSSFTVPEGELTIPQFLPVLKTLAQVV
                     PVDYYMPGCPPESHQIAAVIDLVIKVLHGEEQLPPVGSVIGVGASTVCDDCPRERNVK
                     KITEFVRIQNVAHIDPKLCLLEQGIPCNGPATRQGCGALCPQVAAQCIGCYGPADGVL
                     DYGARLITAFASVIDANEPAEIDRILDSLPDPAGQFYRFNLADSLLRAAKPAWLAKAP
                     N"
     gene            1750..3219
                     /locus_tag="VFF70_11175"
     CDS             1750..3219
                     /locus_tag="VFF70_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HZY45300.1"
                     /translation="MQRITIDPITRLEGHGKIEIFLNEEGDVANTYFQIPELRGFERF
                     CVGRPVEEMPLLTNRICGVCPEAHHMAAAKAADAVYHVDPPRTAKMLRELLYSGFYFT
                     DHTTHFYALAGPDFVMGPDAPVAERNILGVIHKVGLEIGGKVIQARKHGHHIVEIIGG
                     RKVHPCTSIPGGLTKGITKDEANEIADIGKWAIEFAQFSLKLFNDIVLGNKTYLDLIL
                     GDIYTHHTYYIGMVDENNKVNFYDGKVSVVDPDGQRIGKYAASEYTDWIAEHVEPWTY
                     LKFPYLKKIGWKGFVDGKDSGVYMATPLSRLNAAEGMATPLAQEQYEKFYETLGGKPV
                     HQRLATHWARLIELLYAAERIVELATDPEIYSNNFHTIPTEIPTEGVGIVEAPRGTLT
                     HHYTTDERGLLTKVNLIVGTTNNYAPMSMSIKKAAQSLITKGTIVTEGLTNMIEMAFR
                     AYDPCFGCATHSLPGQMPLEVTIRGANGEVVDVLKQCCG"
     gene            3270..3761
                     /locus_tag="VFF70_11180"
     CDS             3270..3761
                     /locus_tag="VFF70_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZY45301.1"
                     /translation="MKTLIIGLGNPLLGDDGIGWRVAEEIERRLPKAIETSEISIEVD
                     YSAGGGLSLMERLVGCTEAILIDAIDIGRGPIGSVYRYALDELPNQFAGHTSSTHETN
                     LQMALQVGRALGAALPDRVTVIAIESPHVYDFTEELSPPVAAALPQAIDMVLQQLTLP
                     LEV"
     gene            3766..4233
                     /locus_tag="VFF70_11185"
     CDS             3766..4233
                     /locus_tag="VFF70_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY45302.1"
                     /translation="MISPELLRRYPFFGGLTIEHLKAMAMLAEEVVFEAGETIFETAQ
                     PAESLWFLLEGCVDLHYIVVDELDPKLRKDFFISEINPGEPFGISALIEPHRYTGTVR
                     STCVSRVLKFNAGGLRALCAVDPKIDAALMRQLAAAAMSRLYDTRVQLAAARA"
BASE COUNT         1010 a         1163 c         1250 g          953 t
ORIGIN      
        1 gtcttcagcg acgaacaagt cttatcacag atcgatggac tgctgatgct gaatttggaa
       61 tacatgaaag aagcgcctga gctggtggcg gtttaacgag taacacttgc accacattgc
      121 atccgttgca gtgcaggtgt aagtaacgag taacaagcga tcgatttact cactactcgt
      181 tactcattac tgaggagaac aacaatgact gaacaagatc aattcgaacc cgtcatcatc
      241 ggcttcactt gcaactggtg cagttaccgc gccgctgatc tagccggaac cgcgcgcgtc
      301 aaatacccgc cgaatgtgcg cttgattcgc ttgatgtgtt cgggccggct cgatccgacg
      361 ttcgtgatga aggcactggc ctccggcgcg gacggtgtat taatcaccgg ctgtcatccc
      421 ggcgaatgcc attatatcga ccagaactat aaagcgctgc ggcgttatca cttgttgaaa
      481 cgcgtgctag cgcagctggg catcgagccg aagcgtgtca agttagtgtg ggccagcgcc
      541 gcggaaggcg tgaagctggc gcatgagatc acgatgttgg tcgaagaaat tcgcgcgtta
      601 gggccgctgc attggggcgc agcgaatggc catgaagttc acgatcattt gccgatgatt
      661 gaagaagaaa tggaagtgaa agaaacgctg gaggtagcgg catgaccacg aatggcacga
      721 aaccaaaatt tgcaatgtac tgggcctccg gctgcggcgg ttgcgaaatt gccgtgctca
      781 acatcggtga gaagattctg gatgtcgatg ccaacttcga cgtggtcttc tggcccgtgg
      841 cgatggatgc caagtacaaa gacgttgaag cgctggaaga cggctcgatt ttgttgtgcc
      901 tcttcaacgg cggcatgcgc acacacgaaa acgaagagat ggcgaagctg ttgcgccgta
      961 agagcaagat cctcgtcgcc tttggctcgt gcgccaccga gggctgcatt cccggtctcg
     1021 ccaatttcag ttcgccacag gaaatttttg agacggctta taacacgatc agcacgaaca
     1081 atcccggcca catcattcca ctatcctcgt tcaccgtgcc cgaaggcgag ttgaccatcc
     1141 cccagttcct ccccgtcttg aaaacgctgg cgcaagtggt gccggttgat tattacatgc
     1201 ccggctgtcc gcccgaatcg catcagattg cggccgtgat cgatctggtg atcaaagtac
     1261 tgcatggtga agagcagctg ccgcccgtcg gatccgtaat cggcgtgggt gcatccacgg
     1321 tgtgcgacga ctgcccgcgt gaacgcaacg tgaagaagat cacggaattc gtacgcattc
     1381 aaaatgtcgc gcacatcgat ccgaagctgt gcttgttgga gcagggcatt ccatgcaacg
     1441 gcccggccac gcggcaaggc tgcggcgcgt tgtgcccgca ggtggccgcg cagtgcatag
     1501 gctgctatgg cccggcggac ggcgtgctcg attacggcgc gcgcctcatc acggcgttcg
     1561 cgtcggtgat cgatgcgaat gagccagcag agatcgatcg gattcttgat agcctgcccg
     1621 atcccgccgg acaattctat cgctttaatc tggctgattc actgctgcgc gctgcgaaac
     1681 ccgcctggtt agcgaaagca ccgaactaaa aagccccgct aacaaccggc gagggtagga
     1741 ggataagaca tgcaacgcat cacgatcgac cccatcaccc gccttgaagg tcacggcaag
     1801 atcgaaatct tcttaaacga agaaggcgac gtcgccaaca cttattttca gattcccgag
     1861 ctgcgcggct tcgaaagatt ctgcgtcggg cggccggttg aagagatgcc gctgttgacc
     1921 aatcgcatct gcggcgtgtg cccggaagcg catcatatgg ccgcggccaa agccgccgat
     1981 gcggtgtatc acgtcgatcc gccgcgcacg gcgaagatgc tgcgcgaact tttatacagc
     2041 ggattttatt ttaccgatca caccacacac ttctatgcgt tagccggtcc tgattttgtg
     2101 atggggcctg acgcgccggt agccgagaga aatattttgg gcgtgattca taaagtcgga
     2161 ttggagatcg gcggcaaggt gattcaagcg cgcaagcacg gtcatcacat cgtcgagatc
     2221 atcggcggac gcaaggtgca tccgtgtact tcgattccgg gtggcttgac gaaaggcatc
     2281 accaaagacg aagcgaacga aatcgccgac atcggcaaat gggcgatcga gtttgcgcag
     2341 ttcagtttga agttgttcaa cgacattgtg ctgggcaata agacttacct tgatctgatc
     2401 ctcggcgaca tctacacgca tcacacctac tatatcggca tggtcgacga gaacaacaaa
     2461 gtcaatttct acgatggcaa agtcagcgtc gtcgatccgg atggtcaacg cattggcaag
     2521 tatgcagcca gtgagtatac cgattggatt gccgagcacg tcgagccgtg gacctacttg
     2581 aagttcccat atctcaagaa gatcggctgg aaggggtttg tcgatggcaa agattctggc
     2641 gtgtacatgg cgacgccatt gtcgcgcttg aatgcggctg agggcatggc gacgcctttg
     2701 gcacaagagc aatatgagaa attctacgaa acgctcggcg gcaaacccgt ccatcaacgc
     2761 ctggcaacgc attgggcgcg actgatcgaa ttgctgtacg ccgctgaacg aattgttgaa
     2821 ttagccaccg atccggaaat ttactccaac aacttccaca cgatcccgac cgagataccg
     2881 accgaaggtg taggcatcgt cgaagcaccg cgcggcacgc tgacccatca ctacaccacc
     2941 gacgagcgcg gcctgctgac caaagtgaac ttgatcgtcg gcacgaccaa caactatgcg
     3001 ccgatgagca tgtcgatcaa aaaggcggcg caaagcctga tcactaaagg gacgatcgtc
     3061 accgagggac tgaccaacat gatcgagatg gctttccgcg cgtacgatcc gtgtttcggc
     3121 tgtgcaactc attcgctgcc aggccagatg ccgctggaag tgaccatccg cggcgcgaac
     3181 ggcgaagttg tcgatgtgtt gaagcagtgc tgtggttgat ggtgagcgat ccgtctttcg
     3241 ttatgggtcg ttcgtcatta tcgattcaga tgaagacatt gatcattggc cttggcaatc
     3301 cattgctggg tgatgatggc attggctggc gcgtggctga agaaattgaa cgcagacttc
     3361 cgaaggctat cgagacttcg gaaatctcga tcgaggtcga ttattcggcg ggcggcggct
     3421 tgagcttgat ggaacgtctc gtcggctgta ctgaagcgat cttgatcgat gcgatcgaca
     3481 tcgggcgcgg gccgatcggc agtgtgtatc gctatgcact cgacgagttg cccaatcaat
     3541 ttgccgggca cacttcatcg acccatgaaa caaatttgca gatggcgctg caagtgggcc
     3601 gcgcgttggg cgcagctcta cccgatcggg taaccgtgat cgccattgaa tcaccgcacg
     3661 tgtatgactt cactgaagaa ctaagtccgc ctgtcgcggc agcgctgccg caggcgatcg
     3721 acatggtgct gcaacaactg actctgccac tggaggtatg agacgatgat ttctcccgaa
     3781 ctgctgcgtc gttatccatt ctttggcggg ctgaccattg aacatctgaa agcgatggcg
     3841 atgctggccg aggaagtggt ctttgaagcc ggtgaaacga tctttgagac agcccaacct
     3901 gctgagtcct tgtggttttt gctggaaggc tgcgtcgatt tgcattacat cgtcgtcgat
     3961 gagctcgatc caaaactacg caaagatttt ttcattagcg agatcaatcc cggcgagcca
     4021 ttcggcatct cggcgttgat cgaaccgcat cgctataccg gcaccgttcg ctcgacctgt
     4081 gtcagccgtg ttctaaaatt caatgcgggt ggactgcggg ccttatgcgc agtcgatccc
     4141 aagatcgatg cggcgctcat gcgtcagtta gcagcagctg ccatgtcgcg tttatacgac
     4201 acgcgcgtcc aattggccgc ggcgagagct tgatcgctgc agctctcgac cacggcagcc
     4261 tgccaaacct ataatcagcc tatctgtttg ctcgaataaa aaacgtccga tcgattttcg
     4321 atcggacgtt tgagtcagag gcgacggtgg gaattgaacc cacggtgagg gttttg
//
LOCUS       DASRTJ010000550         7068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_109737,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000550 DASRTJ010000000
VERSION     DASRTJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109737"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1345
                     /locus_tag="VFF70_11190"
     CDS             194..1345
                     /locus_tag="VFF70_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HZY45303.1"
                     /translation="MRQPDAKYLAALRKRYTTARKKERGQILNEFTQTTSYNRKYAIA
                     VLGSPVTRRIQPLQRRRSPEYTAEDARALEKLSTLFDGINAKLLRAALNNELKHLYDA
                     GFLQISAACYQRLKRVSPATIDRLRTRYRRGLPPRVKRGHTKPGTLLKSQIRVRTWAE
                     WNEDRPGFTEMDLVAHDGGDASGDFAQTLDFTDIKTGWTECAAAPNKAQKHVFAALEQ
                     VRARLPFPLLGIDSDNGAEFINDELIRYADHEHLTFTRSRPGRKNDGAHVEQKNWSVV
                     RRFVGDLRYDTPAQVALLNQLYARLHLYINFFLPVVKLKEKIRHGSKVTKKYDEPQTP
                     YQRVLASPAISPKIKTKLRAHYASLDVVRLHPEIEQLVKRLWASGRRLA"
     gene            1756..3384
                     /locus_tag="VFF70_11195"
     CDS             1756..3384
                     /locus_tag="VFF70_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZY45304.1"
                     /translation="MILPPTIPIAFLILGALAILAGHLAHLRRPSLIMSITALLTVAL
                     IWTLRSDSPVSQIIAPWQPVSVFGVPVSFRVDHSAWIIGLGLVLVGVATAFTWQAYPG
                     QTRPAPRAVALLLIASALASVFASNLLTLALAWGLLDTVFTIALLTRSGSQLGRRAAI
                     AIVLNIASTMCVWIAALVIENQHASLYWHLLNAQPGSSGWLVAAAVLRLGLYPLHQWL
                     PIELDQETDRAVLLFVIPAATGLALLARLAISQALPTQSIMPLLAILSALMGAFLARR
                     SPQPRSGLPFIALSYTGLMVISAITPTSVGTLTAATLNWIFVMSALFISRGFKRTAPW
                     WSIGAIVAAASLIGVPGTIGFIVREQTISSLIDSGSWISLLACLIAETLLVASVIRLI
                     FSPAGDSLPINFLRKISFGVALLFTIIPSIALAVLPSTITNVPLLPEMLNKLGVVGWI
                     AWGLPFAGGIALARLLRKPASDTILLLFDPEAEPKWQRWLELDWITSVVSLPIRFITY
                     VVRGLSSVFEGEGSLLWTMLLIVIALVLYSGVIK"
     gene            3398..4066
                     /locus_tag="VFF70_11200"
     CDS             3398..4066
                     /locus_tag="VFF70_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45305.1"
                     /translation="MSDFLKTLSTVPGAVVLIALLITAALLVVLTNWRLLIFALGAQY
                     LLTGLLLSRAVPIELGMVKALVGIMICPVLYITARRVKWGHLEDDEIDNEKSKSRLAW
                     LISPGLPMRLIIATTGVVLSLSLALRNPLPITGDQVVSRDITIGTYALLLLGLLNMAL
                     NENPLKVGLGLLSLLTGFELFYTVVQPALTIVGLLGLTNLIIALAIAYLTIAWATHPH
                     EVQP"
     gene            4063..5490
                     /locus_tag="VFF70_11205"
     CDS             4063..5490
                     /locus_tag="VFF70_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZY45306.1"
                     /translation="MINSPLPLIVLPLLAAVVTFGFQRSATLSALIAAAIPIGLAIFA
                     WVTPLDGSFSFLGRELFLTTGDRLAIVIIFLTSAAVFLGVWRTTPNWTYYPIALAALA
                     AVVAALGARPVNISVIDGRPFDPFQYSVLFMTIAIALSIFPLQGGQPGVASGTLRYMI
                     LMVIALPAFLAAAWTLDQYSQSPDAANLAQASIALLLLGIALWLGLAPFHSWLPKISS
                     EAPPLSSAFVLGVINIGAWFLLLDMLHEIKVLGDPSTFAVLRAAGILSAVVGGSLAFA
                     QRDFGRLMGYAILGDIGVALAALSTHTDAGLTAALIVVIVRIFGLGLMTIGLAIARRY
                     ALRTLPHVQEHPDDTFESMTGLAWRMPWAALAIIVGGFSLAGVPPFAGFTGRWAALQQ
                     VAGTDLLTAIALLISTIGVAIGTLRGLQSVLKPIEPADTDPTRESRINIALILGALAL
                     SLIIGLFPDVIAPALKQMVAAFAIK"
     gene            5639..>7068
                     /locus_tag="VFF70_11210"
     CDS             5639..>7068
                     /locus_tag="VFF70_11210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZY45307.1"
                     /translation="MTIDQPATAAETFAQLGLSAGLIQTLDELGYEAPTPIQLKTIPL
                     MLAGRSLIGQAQTGTGKTAAFALPILDRLDLKHNTVQALILAPTRELAIQVAEAIQTY
                     SKHLGRVRILPIYGGDSMSKQISRLKAGLHIVVGTPGRIMDHLRRGTLDLAAVKTVVI
                     DEADEMLRMGFIENVEWILGQTPADRQTALFSATMPREVRRIADRHLYNPVAIEIKHK
                     TMTVPTVEQTYVHVLEKQKVDALTHLLEAEAVQGEAILIFVRTKVGAADLVDKLQARG
                     YAAEAMHGDMNQSQRETVIRRMRDGQVEIVAATDVAARGLDIEHIGRVINFDIPNDPE
                     SYVHRIGRTARAGRAGKAILFVTPRETRMLREIERFTGQRLTAMKVPSRADVAARRTT
                     LFKARLLKTLQEEELEVYLELVEEIAEESGRDLAEIAAAAVRLARGDKPLDLPAASEP
                     EPTFDALAASTTGAVVRLYLEAGRSSG"
BASE COUNT         1420 a         1964 c         1982 g         1702 t
ORIGIN      
        1 aacgcgcgag attataccat attttgtata gggctttaca atgaacgcat tgactcactt
       61 aaaaccttac cgaaagtgat tcgtttcctc aaataagaac ctgaccgaaa ccgattcgtt
      121 tcctcacgta agaatctgac cgaaacgagg gcggtgtctc atggtatagc tggcggcaaa
      181 aaggagcaag accatgagac agcctgacgc taagtatctg gccgcgctgc gaaaacgata
      241 tacgaccgcg cgtaagaaag agcgcggcca gatactcaat gaatttacac agacgacaag
      301 ctataaccgg aaatacgcca tcgcggtcct gggcagccca gtgacccgcc gcattcagcc
      361 gcttcagcgt cgacggtcgc cggagtatac agccgaagat gcacgcgcgc tggaaaaatt
      421 gagtacactc ttcgacggga tcaatgccaa gttgttgcgg gcggccctga ataatgaatt
      481 gaagcatttg tatgacgctg gttttctgca gatcagcgcg gcttgttatc agcggttgaa
      541 acgcgtcagt ccggcgacga tcgaccgact gcgcacgcgt tatcggcgcg ggctgccccc
      601 gcgcgtcaag cgcggtcaca ccaaaccggg cacgttgctc aagagccaga ttcgcgtgcg
      661 cacctgggcc gagtggaatg aagaccggcc cggcttcacc gaaatggatc tggtggccca
      721 tgacggtggc gacgcgagtg gcgactttgc ccaaacgctc gactttaccg acatcaaaac
      781 gggctggacg gaatgcgccg cggcccccaa taaggcgcaa aaacatgtgt ttgccgcgct
      841 ggagcaagtc cgggcgcgtc tgccgtttcc gttgctcggc atcgattcgg acaatggcgc
      901 cgagttcatc aacgatgaac tgatccgcta tgccgatcac gaacacctaa cctttacgcg
      961 cagccgacct ggtcgcaaga acgatggcgc ccacgtcgag caaaagaatt ggtccgtcgt
     1021 gcgccgcttc gtcggcgatc tgcgctatga cacgcctgcc caagtcgcgc tgcttaatca
     1081 attatacgcg cgcctgcatc tctatattaa cttcttcctg ccggtggtga aacttaagga
     1141 gaagatccgc cacgggagta aggtgactaa aaaatacgat gagccccaga cgccctatca
     1201 acgcgtcttg gccagtccgg ctatctcgcc gaagatcaaa actaaacttc gggcccacta
     1261 tgcgtctctc gatgttgtcc gcctccatcc cgagatcgag caacttgtta agcggctttg
     1321 ggctagtggt cgacgactag cgtgatgcac tgacgaccta tcgaaggcca cgggtcacga
     1381 tccggtgacg accgtgctcc agctcgtgag cgtccgggac gcaaaaatct cctcgccgct
     1441 ttcggtcaga ttcttatttg aggaaacgaa tgtccttcag ttagctttaa agtgaggaaa
     1501 cgactgactc gttcggtcag ctttttcgtg aggaaacgaa gagcgtttcg gtcaggttct
     1561 tacgtgagga aacgcgaatc catcatatgc acttgcctga aattttatgc cgcacttgag
     1621 tcaggttctt gcatgaatcg accgagcggg ttacaatatc gccgctcccc attcgaacgg
     1681 attacccgaa ctttctccga atggtttgcc gatcgatctg cgcgatcgcg taaattcaat
     1741 tttgttcctt agcttatgat tctgccacca acgattccaa ttgcattctt gattctgggc
     1801 gcactggcca tattggccgg acatctggcg cacctgcgcc ggcccagctt gatcatgtcg
     1861 atcacggcgc tgctcaccgt ggcgctgatc tggactttgc gcagcgatag ccccgtatca
     1921 caaatcatcg cgccgtggca gcccgtatcg gtgtttggcg tgcccgtcag tttccgcgtc
     1981 gatcattctg cgtggatcat tggcctgggt ttagtcttag tcggtgtggc aacagccttc
     2041 acatggcaag cttatcctgg tcaaactcga ccggctccgc gcgcagtcgc tctgctgctc
     2101 attgcaagtg cgctcgcgag cgtcttcgcc agcaatttgt tgacattggc cttagcgtgg
     2161 ggattgctcg acacggtatt taccatcgca cttttaaccc gcagcggatc gcagctcggc
     2221 cggcgtgcgg ccatcgcgat tgtattgaac atagcctcaa cgatgtgtgt ttggattgcg
     2281 gctttagtaa tcgaaaatca acatgcgagc ttgtattggc atttgttgaa tgcgcaaccc
     2341 ggctcaagtg gttggttagt tgctgccgca gtcttgcgct taggtttgta tccactgcac
     2401 caatggctgc caatcgagtt ggaccaggag accgatcggg ccgtgctgct gtttgtgatt
     2461 ccagcggcga ccggtctggc gctcttagcg cgcttggcca tatcgcaggc gctgcccact
     2521 caatcgatca tgccgttgct cgccattctc agtgctttga tgggcgcatt tctagcccgg
     2581 cgcagcccgc agcctcgatc cggtttgcct ttcatcgcct tgagctatac cggcctaatg
     2641 gtgatcagtg ccataacacc aacctcagtc ggcactttga cggccgctac gttgaattgg
     2701 atcttcgtga tgagcgcgtt gtttatcagt cgcggcttta agcgcacagc accatggtgg
     2761 tcgatcggcg cgatcgtcgc agcggcgagt ttgatcggcg taccgggcac aatcgggttt
     2821 atcgtgcgcg agcaaacgat cagcagcttg atcgattccg gatcgtggat cagcttactg
     2881 gcttgcttga tcgctgaaac cttattagtt gcttccgtca tccgattgat cttctcgccc
     2941 gccggtgatt cacttccgat caacttcctc cgcaagatca gttttggtgt ggcgttgctt
     3001 ttcacgatta tcccatcgat cgccttagct gtgctgcctt caacgatcac gaacgtaccg
     3061 cttctgcctg aaatgctgaa taagttaggc gtggtcggtt ggatcgcgtg ggggttgcca
     3121 ttcgccggtg ggattgcctt ggcgaggttg ctgcgcaaac ctgcgtctga cacgatcttg
     3181 ctgttgttcg atccggaggc agaaccgaag tggcagagat ggcttgaact cgattggatt
     3241 acatccgtgg tgtcgctgcc aatccgcttc attacctacg tggtgcgcgg actatccagt
     3301 gtcttcgagg gtgaaggcag tttattgtgg acgatgctgt tgattgtcat tgccctggtc
     3361 ttgtatagtg gagtgatcaa atagcagcta gcatattatg agcgactttc tcaaaacact
     3421 ttccaccgtg cccggcgccg ttgtcttgat cgctttgttg atcacggcgg cgctgctcgt
     3481 cgtgttgaca aactggcgct tattgatctt cgcattgggc gcccaatatt tactaaccgg
     3541 cctactgctt tcgcgcgccg taccgatcga gttgggcatg gtcaaagcgc tggtcggtat
     3601 catgatctgc cccgtgttat atatcactgc gcggcgtgtg aagtggggtc atcttgaaga
     3661 cgacgaaatc gacaacgaaa aatcgaaatc acggctggcg tggttgatct cccccggtct
     3721 gccgatgcgt ttgatcatcg ccacgaccgg cgtggtactc agtttgagcc tcgctctccg
     3781 caatccactg cccatcacgg gcgatcaagt agtctcgcgt gatattacca tcgggacata
     3841 cgctttacta ctgctgggct tgctgaatat ggcgctgaat gagaatccgc tcaaggtggg
     3901 cttgggctta ttgagtttat tgaccggctt cgaattgttt tatacagtgg tccaaccagc
     3961 cttgacgatc gtcggtttgc tgggcctgac caatctgatt atcgcgctgg cgattgcgta
     4021 cctcaccatt gcctgggcca ctcatccgca cgaggttcag ccgtgatcaa tagcccgctg
     4081 ccattgatcg tgctgccctt gctagcggcc gtcgtcacgt ttggctttca acgcagcgct
     4141 accttgtcgg ccttgatcgc cgccgccatt ccgatcgggt tggcgatctt cgcgtgggtc
     4201 acgccgctgg acggttcttt cagttttctc ggacgcgagt tgttcctaac caccggcgat
     4261 cgattggcga tcgtgattat atttctgact tcggcggcgg tgttcctggg cgtttggcgc
     4321 actacaccga attggactta ctatccaatc gcgttagctg ctttggccgc agtcgtcgcg
     4381 gccttagggg cgcggccagt gaacatctct gtcattgacg gtcggccttt cgatccattc
     4441 caatactccg tgctgttcat gaccattgca atcgcgctat cgatttttcc cttgcaaggc
     4501 ggccaacccg gcgtagcgag cgggacgctg cgatatatga tcttgatggt cattgccctg
     4561 cccgcttttc tggccgcagc ttggacgctc gatcaatatt cgcaaagccc tgatgctgcc
     4621 aatttagctc aagcctccat cgctttgttg ctgctcggca tcgcgttgtg gttaggcctt
     4681 gcacccttcc attcatggct gccaaaaatt tccagcgaag cgccgccgtt atccagtgcc
     4741 ttcgtgctgg gtgtcattaa tatcggcgcg tggttcttgc tattagacat gctgcatgaa
     4801 atcaaggtgc tgggtgatcc atcgaccttc gccgtgcttc gagcagccgg catcttatct
     4861 gcagtcgtcg gcggcagtct ggcttttgcg cagcgcgact tcggccgctt gatgggctat
     4921 gcgatcctgg gtgacattgg ggtggctctt gccgcgctca gcacacacac ggatgcgggc
     4981 ttgactgcgg cattgatcgt cgtcattgtg cgcatcttcg gcttgggctt aatgacgatc
     5041 ggtttggcga ttgcccggcg gtacgcactg cggactttac cgcatgtgca ggaacatccc
     5101 gatgatactt ttgaatcgat gacggggttg gcctggcgca tgccgtgggc cgcgctggcg
     5161 atcatcgtcg gcggattttc attggcaggt gtgccgccct tcgctggttt caccggtcgc
     5221 tgggcggcgc tgcaacaagt ggccggtact gatctactca cggcgatcgc attgctgatc
     5281 agcacgatcg gggtggcgat cggtacgctg cgcggtttgc agagcgtgct aaaaccgatc
     5341 gagccagctg acaccgatcc gacgcgcgaa tcgcgcatca acattgcgtt gatcttaggc
     5401 gcactcgcgc tgagtttgat catcggactg ttcccggatg tgatcgcacc cgctttaaag
     5461 caaatggtgg cggcgttcgc gatcaagtag tacgtattgc atagtgcgta aatggaaact
     5521 tcccgtcgat caattccgcg ggaagttttt ttgttgcgcc gaaatttttt caggcatcac
     5581 tgggactact gtataatatt gatcagaatt ccacgccacc aacatgaagg actcccacat
     5641 gaccattgat caacccgcta ctgccgctga aacgtttgcc caactcggct taagtgcggg
     5701 gcttattcaa acactcgatg aacttggcta cgaagcgccc actcctattc agctgaagac
     5761 gattccgctg atgctggccg gccgcagtct catcggtcag gcgcaaaccg gcaccggcaa
     5821 gactgccgcg tttgcgctgc ctattctcga tcggctcgat ctgaaacaca atacggtgca
     5881 ggctctcatc ctcgccccta cccgcgaact cgcgattcaa gttgcggaag cgatccaaac
     5941 ttattctaaa catctcggcc gcgtgcgcat attgccgata tatggtggcg attcgatgag
     6001 caagcagatc agtcgactga aggccggctt gcacattgtc gtcggcacac cgggccgcat
     6061 catggatcac ttgcgacgcg gcacgctcga tctggccgcc gtgaagacgg tcgtgatcga
     6121 cgaagccgac gagatgctgc gcatgggttt cattgagaat gtcgaatgga tcttagggca
     6181 aacaccagcc gatcggcaga ccgcgctctt ctcggccacc atgccgcgcg aggtgcgtcg
     6241 cattgccgat cggcatctgt acaatccggt ggcgatcgag atcaaacaca agaccatgac
     6301 cgtcccgacc gtcgagcaga cctatgtgca tgtgcttgaa aaacaaaaag tggatgcgct
     6361 tacgcatttg ctggaagccg aagccgtgca gggcgaagcg atcttgatct ttgtgcgcac
     6421 gaaagttggc gctgctgatt tagtggataa gttgcaggca cgtggttacg ccgccgaggc
     6481 aatgcacggc gatatgaatc agtctcagcg cgagacggtg attcgacgga tgcgtgacgg
     6541 gcaggtggag attgtggccg ccaccgatgt cgccgcgcgc ggactcgaca tcgaacacat
     6601 tggccgcgtg atcaacttcg atattcccaa cgatcccgaa tcgtatgtcc atcgcattgg
     6661 ccgcaccgcg cgcgccggtc gagcgggcaa ggcgatctta ttcgtcacgc cgcgcgagac
     6721 acgcatgctg cgcgagattg aacgtttcac cggccagcga ctgacggcga tgaaagtgcc
     6781 cagccgcgcc gatgtggccg cgcgccgcac caccctattt aaggcgcgtt tactgaagac
     6841 acttcaagaa gaagaactgg aagtttattt ggagctggtt gaagagatag ccgaggagag
     6901 tggacgtgac ttggcggaga ttgcggcggc cgccgtgcgc ctggctcgtg gcgataaacc
     6961 attggacttg ccggctgcat ccgaaccgga gccgacattc gatgcgctgg ctgccagcac
     7021 gacgggagca gtggtacgat tgtatttgga agcgggacgt agctcggg
//
LOCUS       DASRTJ010000551         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_355495,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000551 DASRTJ010000000
VERSION     DASRTJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355495"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFF70_11215"
     CDS             complement(<1..324)
                     /locus_tag="VFF70_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZY45308.1"
                     /translation="MKWVTRSHVHVDRVACPWLITRFIDNEAEFVFVPKNRVAQTVQT
                     LGAIPFDIPGVELGHQSGRCSFESIIRQYDLQEPGLLRLAKIVHAADIDADIDKDPIA
                     RGLEAI"
     gene            428..1291
                     /locus_tag="VFF70_11220"
     CDS             428..1291
                     /locus_tag="VFF70_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45309.1"
                     /translation="MTDLNPGEYDATLNGIRFHYTLRGSGPALIAISGGPGFDARAWD
                     DFAKIDQFVTVIQHHPRGSGLSEPASNDAYLLADYASDVEELRKHLKLDKPIVMGWSH
                     GGMIAQRFAFTYPDSLSKLILKDTAAYFGEFLSDVEASVTEFKDQLWYADSLAALKQE
                     WANDYQTDEDMARLWTREAKFYFKHYGAREAAYNERIKDLSIRIAPLRAFNEKEAPTL
                     DLRPHLNQIKVPTLVIVGRHDFITNVAMAEELVKHIPHARLEIFEDSGHFAVIEEPEK
                     FYRVVKEFVLS"
     gene            1401..2633
                     /locus_tag="VFF70_11225"
     CDS             1401..2633
                     /locus_tag="VFF70_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZY45310.1"
                     /translation="MSTDLRDRLRRLGVHKGAANVTPKPKRKSHLESLITGEVIETDY
                     GPTFIHTERYAPDHVHGLRPLRELLDLPGSIAARLAGLDPEEMNLRRTVFIDTETTGL
                     SGGTGTLAFVVGVGMFDDEDRFKLHQYFLRNPNEEAAMLLHLSETLDQYTSLVSFNGR
                     GFDLPLLQTRFTLARMRPKILTAPHLDLLPPARRVWKGRYESCSLSTLEYHVLQVQRE
                     QADVSGALIPQMYFDYQRSGDASEMPRVMYHNALDILSMVSLSTHLIQLLARSTEAIE
                     DSADLFALGKWAADRGELDRAELLLRQAIDRSDDLKIKNEARSRLASIYKQLDRRDQA
                     IEVWQELAQHPASHRIDLSITACIELAKHFEWQAIDLKRAIKWTQRGLKLAEDFPDQL
                     LLQELRHRSDRLERKLQK"
BASE COUNT          650 a          730 c          677 g          594 t
ORIGIN      
        1 gatcgcttcc aacccgcgtg cgatcggatc tttgtcgatg tccgcatcaa tatccgccgc
       61 atgcacgatc ttcgccagcc gcagcaagcc cggctcctgc aaatcatatt gccggatgat
      121 cgattcgaac gagcagcggc cactttgatg acccaactcc acgcccggta tatcgaatgg
      181 aattgcgcca agggtctgca ccgtttgagc aacccgattc ttgggcacga acacgaactc
      241 tgcttcatta tcgatgaagc gcgtaatcaa ccacggacat gccacccgat caacgtgaac
      301 gtgcgaacgt gtcacccatt tcattctgcg cctccaggaa gaaattttca aacaagatga
      361 ttatagcgtg tttagccaat aattgagtgt ggtatgattc aaacagcaca ctcccatgga
      421 gaacaacttg accgatttga atcccggcga gtatgacgcg acgctgaacg gcatccgatt
      481 ccactacacg ctacgcggat ccggtccggc gctgatcgcc atctccggcg gacccggttt
      541 cgatgcacgc gcgtgggatg attttgccaa gatcgatcaa ttcgtcacgg tcattcagca
      601 tcatccacgc ggatcgggtc tatccgaacc ggcatcgaat gacgcgtatc tgttagccga
      661 ttatgcatcc gacgtcgaag aacttcgcaa acatttgaag ttggacaaac cgatcgtgat
      721 gggctggtcg cacggcggca tgatcgcgca gcggtttgct ttcacttatc ccgattcatt
      781 atcgaagttg attctgaaag acactgccgc ctacttcggc gaatttctga gcgatgtaga
      841 agcatcagtt accgaattca aagatcaact gtggtatgcc gattcgctcg cggcgctgaa
      901 gcaggaatgg gcgaacgatt atcaaaccga tgaggatatg gcgcgcctgt ggacgcgcga
      961 agcgaagttt tacttcaagc actacggcgc gcgcgaagca gcctacaatg agcgcatcaa
     1021 agatttgtcc atccgcattg cgccgttgcg ggcattcaac gaaaaagaag caccgacgct
     1081 ggatttgcgt ccgcatttga atcagatcaa agttccaacc ttagtcatcg tcggtcgaca
     1141 tgatttcatc accaatgtcg caatggccga agagctggtc aagcacattc cacacgctcg
     1201 attggaaatc ttcgaagact ccggtcactt tgccgtaatt gaagagccgg aaaaatttta
     1261 tcgagtcgtt aaagagtttg tattaagtta gatttgtcat tccgagcgaa gcgaggaatc
     1321 tctacccccg ttaacatcag agttcgcaga ggcagagatt cctcggccta cggccccgga
     1381 atgacacgac agccattaat atgtccacag acctccgcga tcgattgcgc cgactaggcg
     1441 tgcataaagg cgcagccaac gtcacgccca aacccaaacg caagagtcac cttgaatcgc
     1501 tgattaccgg tgaagtgatc gagactgact acggcccgac gttcattcac accgaacggt
     1561 atgcacctga tcatgtgcat ggcctgcggc ccttacggga gttgctcgat ctgccaggat
     1621 cgatcgccgc gcgcttagcc ggactcgatc ccgaagagat gaatctacgc cgaaccgttt
     1681 tcatcgatac ggaaacgacg gggctatccg gcggtacagg caccttggcg tttgtcgtcg
     1741 gtgtgggcat gtttgacgac gaagatcgat tcaagctgca tcaatatttt ttaaggaatc
     1801 ctaacgaaga agcagccatg ctgctgcatc tctctgaaac gctcgatcaa tacacatcgc
     1861 ttgtatcctt caacggccgc ggcttcgatc tgccattgtt gcaaactcga ttcaccttgg
     1921 cgcggatgcg gcccaagatt ttgactgcgc cgcacctcga tctgctgccc cctgcccgtc
     1981 gagtctggaa gggacgttat gagtcctgca gtttatcgac gcttgaatat cacgtcctgc
     2041 aagttcagcg cgaacaggcc gatgtatccg gcgcgctgat cccgcaaatg tacttcgact
     2101 atcaacgcag cggcgatgcg agcgagatgc cgcgcgtgat gtatcacaac gcgctcgata
     2161 tcctatcgat ggtgtcgtta tccacgcatt tgatccagtt gctcgctcga tcaaccgaag
     2221 cgatcgagga ttcagccgat ctgtttgcgc tcggtaaatg ggctgctgat cgcggcgagc
     2281 ttgatcgagc ggagctgctt ttgcggcagg cgatcgatcg ctcggatgat ctgaagatca
     2341 agaacgaagc ccgatcacgc ttagcgtcaa tttacaaaca acttgatcgg cgtgatcaag
     2401 cgatcgaggt gtggcaggaa ctggcgcagc acccagcctc gcaccggatc gatctatcga
     2461 ttaccgcttg catcgaatta gccaagcact ttgaatggca ggccatcgat ctcaaacggg
     2521 cgatcaaatg gacgcagcgc ggcctcaagt tagccgaaga ttttcctgat cagctgctgc
     2581 tccaggagtt gcgacatcga tccgatcgat tggaacgtaa gttgcagaaa tagcaacaaa
     2641 aaacccctca a
//
LOCUS       DASRTJ010000552         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_279998,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000552 DASRTJ010000000
VERSION     DASRTJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279998"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFF70_11230"
     CDS             <1..307
                     /locus_tag="VFF70_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45311.1"
                     /translation="LFTQPPKLTQSQAIDREREQRVSSREPIDYSYTIFWTKTARLWP
                     LNQRSSVAKNLETLINSADFKDNPFDRHYMLDGVEGSHSGASLIALKKVLEALNNER"
     gene            339..1154
                     /locus_tag="VFF70_11235"
     CDS             339..1154
                     /locus_tag="VFF70_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45312.1"
                     /translation="MPELRDITQLIVKHLPPSADLPHVRPLDDRSKRWCMALNRQHAI
                     KTSSTADVDATVTSDVAHARAAFTYLRPNGRAIFLIPNKAGVTTELLVEALSSNGLTR
                     ILTESVLGDSFILARGERPTDQSRPTDRIAAIARIDASSIEILNLDQAAHRYPHVHLL
                     VRQDPPSRGWDDRQPNATWQAVTLHDTQSDQVALLAFTSLVKATAFMQPAVLADVIQG
                     VNKLPRFETGRLIDRAEAVIINPAFGTLREDQRFDFNSPAIKIDPALGLKSNE"
     gene            1184..1804
                     /locus_tag="VFF70_11240"
     CDS             1184..1804
                     /locus_tag="VFF70_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45313.1"
                     /translation="MPDKLCPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYRYHSPL
                     ISAEWCAFTNFPWGRGLINFEPHEEAQAMETYHTWLRLFELQRYYSWIIDRFHISTRA
                     YQLQTYHKDYDFCWIEDRLNALSFRLIFCSRSADSFAAAREERLEVSGKPAQYDDLSI
                     FIHEQELMRRLIDESILPKLEVDVTDNNINRAVEQIADWLEQTDGL"
     gene            1898..2716
                     /locus_tag="VFF70_11245"
     CDS             1898..2716
                     /locus_tag="VFF70_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HZY45314.1"
                     /translation="MTETDTVQNTPTPRTRESLAHDLKTLGLQAGMTAIVHSSLSALG
                     WVSGGSIAVVQALMDVLTLDGTLVMPTHSGDYSDPSQWHYPPVPEDWWPIIRATMPAF
                     DPAIAPTRSMGRIVETFRTWPNVKRSSHPQVSFAAWGKHADFITADHSLDHSLGDRSP
                     LARIYDLDGWVLLLGVPYGNNTSFHLGEYRAGKNRSMVQGGPIIEFGQRVWKEFNDIQ
                     WDADRFDELGADFEKADPIKIARVGSAESRLFSQRSAVDFVQEWFTEKSRDDLP"
BASE COUNT          653 a          769 c          749 g          690 t
ORIGIN      
        1 cttattcact caaccaccga aactcacgca atcgcaagcg atcgatcgcg agcgcgagca
       61 gcgcgtctct tcacgcgaac cgatcgatta ctcctacacg atcttctgga ccaagaccgc
      121 gcgcctgtgg ccgctcaatc aacgctcgag cgtggcgaag aatcttgaga ctttgatcaa
      181 ctcggctgat ttcaaagaca atcccttcga tcgacattac atgctcgatg gagtggaagg
      241 ttcacattct ggcgcaagtt tgatcgccct aaagaaagtg cttgaggcac tcaataacga
      301 gagatgagta atttgctttt tactcgttat tccttatcat gcccgaactc cgcgacatca
      361 ctcaactcat tgtgaaacat ctgccgccca gcgccgatct gccgcacgtc cgcccgctcg
      421 acgatcgcag taagcgttgg tgcatggcgc tcaatcgaca gcatgcgatc aaaacctcaa
      481 gcactgctga tgtcgatgcg accgttacgt cggatgtggc gcatgctcga gcggcgttca
      541 cttatttgcg gcctaatggc cgcgcgatct ttttgattcc aaataaagcg ggtgtcacga
      601 ctgaactgct cgtcgaggcc ttgagttcga atggcttgac ccgcatcctc accgaaagtg
      661 tattgggcga ttcctttatc ttggcgcgtg gcgaacgccc gaccgatcag tcgcgcccca
      721 ccgatcggat tgcagccatc gcccggatcg atgcgagttc gatcgaaatt ttgaacctgg
      781 atcaagcggc acaccgttat ccgcatgtgc atttgctcgt tcgacaagat ccgccatcgc
      841 gcgggtggga cgataggcag ccgaatgcga cgtggcaagc ggttacgctt catgacacgc
      901 aatcggatca agttgcattg ctggccttta cgtcgctggt gaaggcgact gcgtttatgc
      961 agcccgccgt gctggcggat gttattcagg gcgttaataa gctgccgcgc ttcgaaactg
     1021 gtcggctcat cgatcgggcg gaggcagtga tcattaatcc cgcatttgga acattgcgcg
     1081 aagatcagcg tttcgatttc aattcgccag ctatcaagat cgatccggcg ttagggctga
     1141 aatcaaatga gtagggcgag gtcacctcgc ccgacgcaat catatgcccg ataaattgtg
     1201 tccgaaaata attttggaag gcacgcggct gactttcaaa actgacattg cgtttgcttt
     1261 gaacgagcat ccgcgcatcg tcgggccgcg caagtatcgt tatcattcac cgttgatttc
     1321 agcggaatgg tgcgcgttta caaattttcc gtgggggcgc ggcttgatca acttcgagcc
     1381 gcacgaagaa gcacaggcga tggaaacgta tcacacttgg ctgcgcctgt ttgaattgca
     1441 acgctactat tcgtggatca tcgatcgctt tcacatctcg acacgtgcct atcaattaca
     1501 gacgtaccat aaagattacg acttttgctg gattgaagat cgattgaatg cgctcagctt
     1561 ccgcttgatc ttctgcagcc gatcggctga ctcgtttgcc gcagcgcgtg aagagcgttt
     1621 ggaagtttcc ggcaaaccag cccagtatga tgacttatcg atctttatcc atgaacaaga
     1681 actgatgcgc cggttgatcg acgaatcgat cctgccgaaa ttggaagtcg atgttacgga
     1741 taacaatatc aatcgtgccg tcgagcagat cgccgattgg ttagaacaga ccgacggttt
     1801 atgaacacag taaattgatc tcttaaccgc caagcgcgcg aagaaaaaca aactataact
     1861 ctgcgatctt tgcgttctct gcggttcaat ttctaacatg accgaaaccg acacagttca
     1921 aaacacgccc acacctcgca cgcgtgaaag tctagcgcac gatctcaaga ccttgggctt
     1981 gcaagctggc atgaccgcga tcgttcattc atcgctgagt gcgttgggct gggtcagcgg
     2041 tggatcaatt gccgtcgtgc aagcgttgat ggatgtactc acgctcgacg gcacactcgt
     2101 catgcccact cattccggcg attactctga tccatcgcag tggcattatc cgcccgtgcc
     2161 ggaagactgg tggccgatta ttcgcgccac gatgccggct tttgatccgg cgattgcccc
     2221 gacgcgcagc atgggccgca ttgtcgagac gtttcgtacg tggccgaacg ttaagcgcag
     2281 ttcgcatccg caagtttcat tcgcggcgtg gggcaaacac gccgacttca tcaccgctga
     2341 tcattcgctc gaccactcgc tcggcgatcg atcgccgttg gcgcgcattt acgatctcga
     2401 cggctgggtc ttgttactcg gcgtgccgta tggcaacaac acttcatttc atttgggcga
     2461 atatcgcgcg ggcaaaaatc gttcgatggt gcagggcgga ccgatcatcg agtttggaca
     2521 gcgcgtgtgg aaagagttta acgatatcca atgggacgcg gatcgatttg acgaactggg
     2581 cgcggatttt gagaaagctg atccgatcaa gatcgcgcgc gtgggttcgg ctgaatctag
     2641 attgttttcg cagcgctcgg cggttgattt tgtgcaagag tggtttactg aaaaaagtag
     2701 agacgattta ccttagcgct gcccgctagg gcaggtgtga accgcctcta cttgatcacg
     2761 ctaaatgact cttcaatccc agtcgtttaa agacatcctc tcgcttcaac ggaaatctct
     2821 gctgatgccg catagcctct ggcaattcca gattgaatga c
//
LOCUS       DASRTJ010000553         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_262454,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000553 DASRTJ010000000
VERSION     DASRTJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262454"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFF70_11250"
     CDS             <1..295
                     /locus_tag="VFF70_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45315.1"
                     /translation="GFVIVLAVSIGSRLNEQTVTMLMGLMCGAGVALPIGLAVGLFLG
                     DRRRHDREAPTPPIVIMHQPQQSPSSSVPLIQAPYLSPPPRSFSIIGDDGSKD"
     gene            complement(442..1101)
                     /locus_tag="VFF70_11255"
     CDS             complement(442..1101)
                     /locus_tag="VFF70_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45316.1"
                     /translation="MSSSATILLVEGARPAISLVPLLENKGYTVTRVSNSKEAFNRIK
                     TTAPALAVVDCTTLHTDGTKICADLRSHADSFPVVIIVRAGTDLNKIDCADTVLTRPF
                     SPRKLLTRIKHYLPNSTGEVLKAGDFSLNIDGRFLRINKSEVHLTPMQTRLMEAFLRK
                     PNELLSRAHLMKTVWKTSYTGDTRTIEVHVRWLREMIETDPKKPKRLLTVRGMGYRLV
                     I"
     gene            complement(1209..1409)
                     /locus_tag="VFF70_11260"
     CDS             complement(1209..1409)
                     /locus_tag="VFF70_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45317.1"
                     /translation="MVAKRIAVLLLGFAVGVGMTLGIMAIFSDRGMTNLGAPNIVLTS
                     FCFGVAACIALDHVLSTGMIKR"
     gene            complement(1481..>2964)
                     /gene="murJ"
                     /locus_tag="VFF70_11265"
     CDS             complement(1481..>2964)
                     /gene="murJ"
                     /locus_tag="VFF70_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZY45318.1"
                     /translation="YYAAFGLPDLLFTLIAGGALASAFIPIFSAYLSRDDRSGAWRLA
                     SAVINTAFIVSLIASIVLASLAPWIVTQTVGRGFTVEQQQLTANLMRVILIGTVVFSI
                     SGIFMSALQANQHFILPALAPVMYNLGILGGVIFLTPRYGVWGPAIGVVVGAVLHLLI
                     QVPGLIKYHARWTPRLDWSDPDLRRVIKLMLPRIAGLGVVQLAAIIATSLASTLSAGS
                     VTALRYGWQVMQLPETLIATAIATAAFPTLSEFAARKQFTELRATLSSTLRAILLLTV
                     PATLALLILGRPAVQLLFERGKFTSDATDLVTFAVQGYAIGLIGQSLLEVCARTFYAQ
                     QDTATPLFVAIGAMAVNILASVILKGTLGVGGLALANSIGVTIEVLGLLIILRHRLRG
                     VDGRRIARSILKFAAGSIGMLIVVLIAQSIFISLPLHLDSIGSDSLRSALERGLPLIA
                     ASILGAIVYVILMIALRSEELIGLRRSIGRRSPIEITESAAIE"
BASE COUNT          705 a          822 c          833 g          604 t
ORIGIN      
        1 cgggtttgtg atcgtgctgg ccgtatcgat cggcagccgg ttgaatgagc agacggtgac
       61 tatgttgatg ggcttgatgt gcggagcagg ggtggccttg ccgatcgggt tggccgtggg
      121 cctgttcttg ggtgatcgtc gtcgccacga tcgcgaagcg ccaacaccac cgatcgtgat
      181 catgcaccag cctcaacaat ctccatcgag cagtgtgccg ctgattcaag cgccatatct
      241 ttcgccaccg ccccgatcgt tcagtatcat cggcgatgat ggcagcaagg attgagctac
      301 ttaactttgg ggcgccattg cgaggagcat ctgtttgcga cgaagcaatc gatcgattga
      361 aactgagatt gcatcgccct gacgcggctc gcaatgacgg tatagcgtaa aataaaaacg
      421 gtagactcat tgtctgccgt tttagatcac caagcgataa cccatgccgc gcacggtcaa
      481 taaccgtttg ggtttcttgg gatcagtttc gatcatttca cgcaaccagc gcacatgcac
      541 ctcgatcgtg cgcgtgtcgc ccgtgtaact tgttttccag acggtcttca tcaagtgcgc
      601 gcgcgacaaa agttcattcg gcttgcgcag gaacgcttcc atcaaacgcg tttgcattgg
      661 cgtgagatgc acttcacttt tgttaatgcg caagaaacgc ccatcgatgt tcaggctgaa
      721 gtcgcccgct ttcaatactt cgccggtgct gttgggcaga taatgcttga tgcgagtcag
      781 caatttacgc ggcgagaacg gtcgggttaa gaccgtgtcc gcgcaatcga tcttgttcag
      841 atccgttccg gcccggacga taatcaccac agggaaggaa tccgcatgcg atcgcaaatc
      901 ggcacaaatc ttggtgccat cagtatgcag cgtcgtgcaa tcgaccacgg ccagcgcagg
      961 tgcagtggtc tttatgcggt tgaaggcttc tttgctattg ctgacgcgcg tgacggtgta
     1021 gcccttgttc tccagcagcg gaacaagcga gatagccggc cgtgcgcctt caaccagcag
     1081 gatcgtagca gatgatgaca tgttgtttcc agaacggtct acgtcaggga gtgaaagaaa
     1141 caggcgggca tgcgctagag tttgcgctgc ccgcgatgtt ttgagtcaaa aatttggcag
     1201 cagcgtaatc agcgtttgat catacccgta ctgagcacat gatccaacgc aatacatgcc
     1261 gctactccaa aacagaatga tgtcaaaaca atattcggcg cacctaagtt ggtcatgccc
     1321 cgatcgctga aaattgccat gatgcctaac gtcatgccca cgccgacggc gaagcctaat
     1381 aatagaacgg cgatacgttt tgcgaccata gttgtaatct gccctccgct ggatgtgacc
     1441 gcgattgtag tcgatgtctt tcgactcgtc aagtaaccga tcactcgatc gcggcggatt
     1501 ctgttatctc gatcggtgat cggcgcccga tcgatcgacg cagcccgatc aattcttctg
     1561 atcgcagcgc gatcatcagg atcacataca cgatcgcgcc caagatcgag gcggcgatca
     1621 acggcagacc acgttcgagc gcggagcgca gcgagtcact gccgatcgag tcgaggtgca
     1681 gcggcagcga aatgaagatc gactgcgcga tcaaaacgac gatcagcatg ccgatcgagc
     1741 cggcggcgaa cttcaagatc gaacgggcga tgcgccgacc atcgacgccg cgcaaccgat
     1801 ggcgcaggat gatcaacaag cccagcactt cgatcgtcac gccgatcgaa tttgccaagg
     1861 ccaatccacc cacgcccaaa gtacctttca agattacact cgcgagaatg ttgaccgcca
     1921 tcgcgccgat cgcaacgaac agcggcgtgg ctgtatcttg ctgcgcataa aatgttcgcg
     1981 cgcaaacttc gagcaaggat tgtccgatca agccgatggc ataaccttgc acagcgaagg
     2041 tgacgagatc cgtcgcatcc gaggtgaact tgccgcgctc aaaaagtaat tgcaccgccg
     2101 gtcgaccgag aattaaaagg gcaagcgtgg ccggaacggt gagcagcaag atcgcgcgta
     2161 gcgttgaaga gagcgtggcg cgcagttcgg tgaattgttt gcgcgccgcg aactcactga
     2221 gcgttgggaa ggcggcggtg gcgattgccg tggcgatcaa tgtttcgggc aactgcatca
     2281 cttgccagcc gtatcgcaac gccgtgacac tgcccgcact caacgtcgag gctaaactcg
     2341 tagcgatgat tgccgctagc tgcaccacac ctaagcctgc gatgcgcggc aacatcaact
     2401 tgatcacgcg gcgcagatcg ggatcgctcc agtccaagcg cggtgtccag cgcgcatgat
     2461 atttgatcaa gcccggcact tgaatcaaca aatgcaagac tgcgcctacc acgacgccga
     2521 tcgctggtcc ccacacgccg taacgcggtg taaggaaaat cacgccgccc aaaatgccga
     2581 ggttgtacat cacgggcgcg agggcaggca aaataaaatg ttgattcgct tgcagcgcgc
     2641 tcatgaagat gccactgatc gaaaacacca ccgtgccgat caagatgacg cgcattaagt
     2701 tcgcagtcag ttgttgttgc tcaaccgtga agccgcgccc caccgtttgg gtgacgatcc
     2761 acggcgcgag gctagccagt acgatcgatg cgatcaatga aacaatgaac gccgtattga
     2821 tcaccgcact ggcaagtctc catgcgccag atcgatcgtc gcggcttagg taagcggaga
     2881 agattggaat gaaggccgag gccaacgcgc cgcccgcgat cagcgtgaag agcagatcgg
     2941 gtagaccgaa ggccgcgtaa tacg
//
LOCUS       DASRTJ010000554         9440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_338000,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000554 DASRTJ010000000
VERSION     DASRTJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338000"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4863)
                     /locus_tag="VFF70_11270"
     CDS             complement(<1..4863)
                     /locus_tag="VFF70_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45319.1"
                     /translation="MKRSKPPAVTHSRSMKNTILDPVARRDPVPAQSESSAPIAPLIP
                     IEWAGVLDRLPGTVFVLDHSGIILWVNSSITSASGYTREALLGRTLLELLIENEHERF
                     DQWFNAVLTAQPAAHIEPLTLNTYLRRFDDLIDYGCLSGVYDTARQNIVVTYHRLNVR
                     KELDASLQERITRLEVANAAATIVSQSLDPDKALQAVLQKTLEVLHAEAGIIMLLDPD
                     SGDLVFKAQIGWRPYALIDATTRVKANIGLAGVVVQTEVPVVVDDVLADPRISISGFR
                     QSGVRSIALVPMHSRAKVIGVLSVIEYSPRSFVTEDVNILCTIADQIGISIENAQLYQ
                     SEQKQRRLAEALHQVAGVVNSTLNLDTVLDLIVDQLKRVVSYDSVSLWLSTGGDFQLA
                     ITRGDVDWTLANAQFSKLRTTQLLLEKQEPIIILDTTADSIWIPLVKDDPVRSWLGIP
                     LLNKNNDQVLGILEIDNYKPYAYSKEDIQAAVVFATQAAAAIENARLYAESQRRAELM
                     AALNSVSATVSQSLELEPTLRSALDKALEVVGVEAGAISLVDEETQELVIRVHRGWRQ
                     QDLLSGLRIKLGQGLSGQAALTGEVVVTGSLENEPRLAVPAVRDEGVQSMVLAPMHAR
                     GRVVGVLGVMSYRVRMFDRQSIEVVKSIADQIGLAIDNAQLFARESRRAVQLALLNDV
                     ARDVVSTPDLMERLNKTTQVIREKFGYFSVLLFMVDEDGQHLSMRSGAGEQANLVDPN
                     YHQPMGKGLIGSVAQSGEMIMANDVANDPRYLNPLPDRADPVKAELAVPLRLGQQVVG
                     VLDIQQLQINAFTRDDAQVIQSLADQLVVAITNAQLYDQACQRVTELTALQQISLQVT
                     SSLDLWTVLEAITHSVLTLIHAATAHIYLFNADSNELTFSTALWDDNERAAAVLQPQV
                     DDPISQVARTGKLVIDNDRSMHPWGTPPLALGALAALPLKRADRVVGVLLTAFRSPHY
                     FVPDQVRVLMLLADQAAVAIDHARLYTNESRRSTHLSLINAVGRQATSTLDLTTLLDR
                     ASAAIRQSFGYFHVGLLLLDKAANEVLLHAYSGGSPEAYGLGYRQSIDIGLIGWAAKH
                     GETMLANDVTHEPRYFSEGHHPKTIHAELCVPIIRNEEVIGVLNVEDLEFNAFSPDDV
                     RAMETLADQLAIAIENARLYEEANQRVAELAALQEISLQVTASLDTLSVLNTIAQNTL
                     LLIRADDIHIFLYDPDTDSMMFGTALWKDGSREPAIVQPRRNGLTWKVFQEGQPVVIN
                     DAQHHPLYDTDQARAWGLTSIAGFPLKRADAILGVFNVAFKEPHTFSPEEIRVIMLLS
                     DLAAIAVSNARLYEQTKRRLDEISTLHEVSVAATSTLDFGEVTERTVRALQRSLGFEY
                     IALFLVDEDDQHADLYSTSGREAEVERNPRVEVGVGIIGWVIANGKLLNVPDVLDDQR
                     YLAGITTTRSEMCVPLRVGDRVIGAVDLQSPRLSAFSNNDERLLLTVAGQWAVILENA
                     RLYAVERRRRQQLEGLQVTASAIGAELDLNALLNLIVESAASTFSASAVSLLLWSESD
                     KLLNIYASHGLSAEFVAETSVSYTEVKWALDPTQTADGSGDHTSAPNLKPLVIYDLCN
                     NLFDAGQSPLY"
     gene            complement(4860..5198)
                     /locus_tag="VFF70_11275"
     CDS             complement(4860..5198)
                     /locus_tag="VFF70_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45320.1"
                     /translation="MSAIAGPSSVLGLIAALYMLYIFALLSRKLGNVTKMKPHYRGLY
                     LSMALTLVAIVAHVLRVAVWLNPTPMPQVLTTGEFYLVTYYLPLTLAVTISLVVVVRY
                     WGWLFSERDR"
     gene            complement(5195..5485)
                     /locus_tag="VFF70_11280"
     CDS             complement(5195..5485)
                     /locus_tag="VFF70_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45321.1"
                     /translation="MTVIDSVLNLYIWIALCALMIVLFRIARFYQKTSGRRSYYQLFS
                     VPIVLLLAGGLRYVSLSIFVGDGLGDLLMVLGGLCLIGLSYYTLHLMTGGRS"
     gene            complement(5482..6246)
                     /locus_tag="VFF70_11285"
     CDS             complement(5482..6246)
                     /locus_tag="VFF70_11285"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZY45322.1"
                     /translation="MERIKSGVPGLDEMLNGGFLPQSANLVEGASGTGKSTLGMQFIY
                     NGITKFNQPGLIITFEEFPQQYYADAASYGWDLRALEQSNQLRVIMTSPEVSHADLER
                     VDGRIQAMIQEIGAVRILVDSLTHFDRLTDDPVARRGLIFSFINSLKRERLTSLLTRE
                     STTLIGGIEAPEEDVAFVADSYLLLRYVEIESAIRKALLVMKMRGSDHAKDIRQFEIT
                     RAGFEVRSKFEGREGLMSGSPHRMADSFVEAFVKRS"
     gene            complement(6337..6762)
                     /locus_tag="VFF70_11290"
     CDS             complement(6337..6762)
                     /locus_tag="VFF70_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45323.1"
                     /translation="MSDPKSSAFQEWNPAPKRPVGTDLAARLMDDMDPEVLEFLKTSV
                     NSFIKWDLVIFYSENPNTTDTATNVSRYIGRDLSVTESEMDELVQSKVLQRDRVSDLT
                     VYSLTIDQAMRDRIKHFVAVANDVQFRGKVFYHLIRGLR"
     gene            complement(6759..>9440)
                     /gene="secA"
                     /locus_tag="VFF70_11295"
     CDS             complement(6759..>9440)
                     /gene="secA"
                     /locus_tag="VFF70_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZY45324.1"
                     /translation="RRRQLDLRVKKLDDELFTTEDDLLSDFLPRAFAAVRESAKRNVG
                     LRHFDVQLIGGMVLHQGKISEMKTGEGKTLVASLPLYLNALAGHGGHLVTPNDYLSKI
                     GAQWMGPIYHGLGTTVGVIQHESAFVFDPDFKAQDDRYNRLRPVSRRDAYRADITYGT
                     NNEFGFDYLRDNMVWELAQCAQRELQYAIVDEVDNILIDEARTPLIISGPAEESSDQY
                     VRFSEIVRRLRPTTNDDEDHPNGEYTVDLKTRNATLTEAGIERIEQVTGIPNLYAPEH
                     SDLIPYLDNALRANVIFKRDKDYIVKDGQVIIVDEFTGRLMYGRRYSEGLHQAIEAKE
                     KVTVQRENLTYATITFQNFFRMYRKLAGMTGTAKTEEEEFQKIYNLDVVSIPTHREMI
                     REDTPDVIFKTEDAKFRSVVSEIEEMNKQGQPVLIGTVAIETSEMLAGMLKRRGIPHN
                     VLNAKEHEKEAVYIAQAGRVGAVTVATNMAGRGVDILLGGNAEGIARDLLRKKGVDLT
                     EISPADWDQALAEAQRMVATEREQVLKAGGLHIVGTERHEARRIDNQLRGRAGRQGDP
                     GSSRFFISLEDDLMRRFGGDSIKNLMDRFKMDEDQPLEHSLLSRSIEQAQVRVEGYNF
                     DSRKHVLEYDDVVNTQRGVIYAQRRKILNESNLRPIILDMMYDEIQAVIAQHTQAQHE
                     DEWDLSGLMISMRGMIPLPPTLTINDWRGLSVEEIEQQLIEFAEQGYAEKTKQLADLM
                     PQAERMTLLRAVDGHWVRHLTDLDNLREGIGLRAFGQQNPLVAYKREAAEMYDGMLSS
                     IRSQVARDIYRVQAAPQAAALPARQLRTNAPASGGKGAKPIKTSSPDAIGRNDLCPCG
                     SGKKYKHCHGAPGAEPLSASQLAGAGTRSATPVKIRK"
BASE COUNT         2225 a         2736 c         2344 g         2135 t
ORIGIN      
        1 gtagagtgga ctctgccccg catcgaacaa gttattgcac aaatcataga tcaccagcgg
       61 tttgagattc ggcgcgctgg tgtgatcacc ggagccatcg gccgtttgtg tcggatcgag
      121 cgcccatttt acttcggtat agctcacgct cgtctctgcc acaaattccg ccgacagacc
      181 gtggctggcg taaatgttga gcagcttgtc ggactcactc cacaacaaca aactaacggc
      241 agacgcgcta aatgtgctgg cggcgctttc cacgattaga ttgagcaatg cgtttaagtc
      301 caactccgca ccgatcgccg atgcggtgac ttgcagacct tccaattgct ggcgccgccg
      361 ccgctcaacc gcatacagcc gcgcattttc caagatgacc gcccactgcc cggccaccgt
      421 caataacaac cgctcatcat tattggaaaa cgcgctcagg cgcggactct gcaaatcaac
      481 cgcaccgatt acgcgatcgc cgacgcgcag cgggacacac atttccgatc gggtggtggt
      541 aattccagcc aggtatcgct gatcgtcaag gacatccggc acattaagca gtttgccgtt
      601 cgctatcacc caaccaataa tgcccactcc cacttcgaca cgtggattcc gctcaacttc
      661 agcttcacgc ccggaggtcg aatataaatc agcatgctgg tcatcttcat cgaccaagaa
      721 gagcgcgata tattcgaagc ctaacgatcg ttgtaatgcg cgcacggtac gctcggtcac
      781 ttcgccaaag tccaacgtgc tggtggctgc cactgagact tcgtgcaggg tgctgatttc
      841 atctagtcgc cgcttggtct gttcatacaa tcgagcgttg ctgaccgcaa tggcggccaa
      901 atcggacaac agcatgatca cccgaatttc ttctggacta aatgtgtgcg gctctttgaa
      961 agccacattg aaaacaccca ggatcgcatc tgcacgctta agcgggaaac cggcaattga
     1021 cgtcaagccc cacgcccgcg cctgatcggt atcgtacaaa ggatgatgct gcgcatcgtt
     1081 gatcacaacg ggctggcctt cttggaaaac tttccaagtc aggccgttgc ggcgcggttg
     1141 aacgatcgcc ggttcgcgtg agccgtcttt ccacaaagct gtgccgaaca tcattgaatc
     1201 tgtgtcgggg tcatacaaaa agatatgaat atcatcggcc cgaatcagca acagcgtatt
     1261 ctgcgcgatc gtgttcaaga ctgacaacgt atcgagggag gccgtgactt gcaagctgat
     1321 ctcttgcagc gcggccaact cggccacgcg ctggttcgcc tcttcatata agcgggcatt
     1381 ctcgatcgcg atggctagct gatcggccaa tgtttccatc gcgcggacat cgtccgggct
     1441 gaaggcgtta aactccaagt cctctacatt tagcaccccg atcacttctt cattgcgaat
     1501 gatcggcaca cataattcgg catgaattgt cttggggtga tggccttcac tgaaataccg
     1561 cggctcgtgc gtcacatcat tcgccagcat tgtttcaccg tgcttcgccg cccatccgat
     1621 caagccgata tcgatcgatt ggcgatagcc aagcccataa gcctctggtg atccgcctga
     1681 gtacgcgtgc aacaaaactt cgtttgcggc tttatccagg agcagtaagc cgacatggaa
     1741 ataaccaaaa ctctgccgaa tcgctgcgct ggctcgatca agcaatgtcg tcaaatcgag
     1801 tgtggacgtc gcctgccgcc ccacagcgtt gatcaatgac aaatgcgtgg agcgccggct
     1861 ctcattggtg tacaagcggg catgatcgat cgcaactgcg gcctgatcag ccaatagcat
     1921 caacacgcgg acttgatcgg ggacaaagta atgcggcgat cggaaagcag tgagcagcac
     1981 acccacaacc cgatcagccc gtttcagtgg aagggcagca agcgcgccga gtgccagcgg
     2041 tggtgtgccc catggatgca tactgcgatc gttatcaatc accaactttc cggtgcgcgc
     2101 cacctgtgag attggatcat cgacctgggg ttgaaggaca gccgcggcgc gttcattatc
     2161 atcccataac gcagtgctaa aagtcagttc atttgaatcg gcattaaaca agtagatgtg
     2221 agccgtggca gcgtgaatca acgtcagaac ggaatgggta atggcttcca agacggtcca
     2281 caaatccagc gaagaagtta cctgcagact gatctgctgc aacgcggtga gctcggtcac
     2341 ccgctggcat gcttgatcgt acaattgcgc attcgtaatc gccacaacca actgatcggc
     2401 tagcgactga atgacctgcg catcatcccg cgtgaatgca ttgatctgca attgttgaat
     2461 atccaatacg ccgacaactt gttgacctaa acgcagcggc acagccaatt ccgcttttac
     2521 cggatcggct cgatcgggca gcgggttaag atagcgcgga tcattcgcga catcattcgc
     2581 catgatcatc tcgcccgatt gagccaccga accaatcagt cccttgccca tcggctgatg
     2641 gtaattcgga tcaactagat tagcctgctc gccggcgccg gagcgcatac tcagatgttg
     2701 gccgtcctca tccaccatga acaggagcac cgagaaatag ccgaatttct cccgaatgac
     2761 ttgcgtcgtc ttatttaacc gctccataag atcgggcgtt gagacaacat cgcgcgcgac
     2821 atcgttcaat aaagccagct gcacggctcg ccgcgactcg cgggcgaaca actgcgcgtt
     2881 atcaattgcc agaccaatct gatcggcaat cgatttgacc acttcgatgg attgccgatc
     2941 gaacatacgc acgcgataac tcatgacacc caagacaccg acaactcttc cgcgcgcatg
     3001 catcggtgcc agtaccatcg attgcacgcc ttcatcacgt acagccggaa ctgccagtcg
     3061 tggctcattc tccaaactgc ctgtcacgac cacctcgccg gtgagagcgg cctgcccgga
     3121 caacccttga cccaatttga ttcgcaagcc gctcagcaaa tcctgctgcc gccaaccgcg
     3181 atgcactcga ataaccagct cttgcgtttc ttcatctact aagctgatcg cgccggcttc
     3241 cacgcctaca acttccaacg ctttatccag cgcagacctc agcgtcggtt ccaattccag
     3301 tgattgactg accgtggccg aaacactatt gagcgcggcc atcaattccg cgcggcgctg
     3361 cgattcagca tataaccgcg cattttcaat cgccgccgcg gcttgcgttg cgaagacaac
     3421 ggcagcctga atatcttcct ttgaataagc gtaaggcttg tagttgtcaa tctccaggat
     3481 gcccaagact tgatcattgt ttttgttgag cagcggtatg cctagccaag atcgaacggg
     3541 atcatctttg actaacggaa tccaaattga atcagcggtt gtatcgagga taatgattgg
     3601 ttcttgtttt tccagcaaaa gttgagtagt gcgcaacttg ctaaactggg cattcgccag
     3661 ggtccaatca acatcgccgc gcgtaattgc caattgaaaa tcgccgcccg tgctcaacca
     3721 caaagagaca ctgtcatatg acacgacacg ctttaattga tcaacgatca ggtcaagcac
     3781 cgtgtccaaa ttcaaagtgg aattgaccac tccggcgact tgatgcagcg cttcagccaa
     3841 gcggcgctgc ttctgctcgg attgatacag ctgcgcattc tcaatgctga tgccaatttg
     3901 atcggcaatg gtgcataaaa tattgacgtc ctcggtcacg aagctacggg gcgaatattc
     3961 tatgaccgac aatacgccga tcacttttgc ccgggagtgc atgggtacca gcgcgatcga
     4021 tcgaacgccc gattgtctga aaccgctaat gcttatccgt ggatcggcca acacgtcatc
     4081 gaccacaacc ggcacctcgg tttgcacgac gacgccagcc aatccgatgt tggccttaac
     4141 gcgtgtggtc gcatcgatca gagcgtatgg tcgccagccg atctgagcct tgaaaaccaa
     4201 atcgccggag tctggatcta acagcatgat gatgccagcc tcggcatgca ggacttccaa
     4261 agtcttttgc aagacagcct gcagcgcttt atcgggatcc aacgattgac taacgatcgt
     4321 cgcggcggca ttggcgactt ccagacgagt gatgcgctcc tgtaaactgg catcgagttc
     4381 tttacggaca ttcaggcggt ggtacgtgac cacaatgttt tgacgcgccg tatcatacac
     4441 accggacaag catccataat cgattagatc gtcaaaacgg cgaagatatg tgttgagagt
     4501 gaggggttcg atgtgtgcag caggctgagc cgtcaacaca gcgttaaacc attgatcaaa
     4561 acgctcgtgc tcattttcga tcaacaattc aagcagcgtg cggcccagca gcgcttcacg
     4621 cgtataccca gacgcagatg tgatcgatga gttaacccag aggataatgc cagaatgatc
     4681 gaggacaaag acagttccgg gcaaccgatc gagtacgcct gcccactcga tcgggatcag
     4741 cggcgcgatc ggggctgaag attcagattg ggctggcacc ggatcccgcc gtgcgactgg
     4801 atcgagtatc gtgttcttca tcgagcgact gtgtgttacc gcaggcggtt tggaccgctt
     4861 catcgatctc gctccgagaa taaccaaccc caataacgca cgacgacaac caggctaatc
     4921 gtcaccgcca gcgtcagcgg cagataatag gttaccaaat aaaattcacc tgtcgtcaat
     4981 acttgcggca tcggcgttgg gttcaaccag acggcaacgc gcaatacatg cgcgacgatg
     5041 gcgaccaggg tcaaggccat actcagatat aaaccgcgat aatgcggctt catcttggta
     5101 acattgccca acttacgact gagcagggca aagatataca gcatatagag cgcggcaatt
     5161 aagccgagca cggaagacgg gccggcgatg gcgctcatga acgcccgccg gtcatcaaat
     5221 gcaaagtgta gtaactcaaa ccgatcagac acaagccgcc cagcaccatg agtaagtcac
     5281 ccagtccatc cccgacaaaa atgctcagcg acacgtagcg caagccgccc gctagcagca
     5341 aaacgatcgg cacactaaat aactggtaat acgatcgccg gcctgaagtt ttctggtaga
     5401 agcgcgcgat ccgaaacaag acaatcatca acgcgcacag cgcgatccag atatataaat
     5461 tcaacacgct atcgataacc gtcatgagcg tttgacaaaa gcctcaacaa acgaatcggc
     5521 catgcggtgc ggactgccac tcatcaagcc ctcgcgccct tcaaatttcg atcggacttc
     5581 gaaaccggcg cgggtaatct caaactgccg gatgtctttg gcgtgatcgc tgccgcgcat
     5641 tttcatgacc agcaacgctt tgcgaatggc gctttcaatt tcaacgtagc gcagcagcaa
     5701 gtatgaatct gccacaaaag ccacgtcttc ttccggcgct tcaatcccgc caatcaacgt
     5761 cgtgctctcg cgtgtcagta aactggtcaa ccgttcgcgc ttaagcgaat tgatgaaact
     5821 aaagatcagg ccgcgccggg ctacgggatc atcggtcaat cgatcgaaat gagtcaagct
     5881 atcaaccaag atccgcaccg cgccaatctc ctgaatcata gcttgtattc ggccatccac
     5941 ccgctctaaa tccgcatgac tgacttccgg gctggtcatg atcacgcgca gctgattcga
     6001 ctgttccagc gcgcgcaggt cccaaccata gctagccgca tcagcatagt attgctgcgg
     6061 gaattcttca aaagtaatga ttagtcccgg ctgattgaac ttggtaatgc cattgtagat
     6121 gaattgcatg ccaagcgtgc ttttgcccgt gcccgacgcg ccttccacca gattggccga
     6181 ttgcggcaag aagccgccgt tcagcatttc atccaagcca gggacgcccg attttatgcg
     6241 ttccatattt acacctcctt cgatcgcttg acaatctcgc gaatgatctc aggctgatcg
     6301 accattcgtc gattcaactt tggcaaggtt atttgttcag cgcaagccgc ggattaaatg
     6361 atagaagact ttgccacgaa actggacatc gtttgccaca gcgacaaagt gcttaatgcg
     6421 gtcacgcatc gcctgatcaa tagtcaatga atataccgtg agatcactga cgcgatcgcg
     6481 ctgcaatact ttcgattgca ccagttcatc catttcactc tcggtcacgc tcaaatcacg
     6541 cccgatatag cgtgacacgt tcgtcgcggt atccgtggtg ttgggatttt cggaatagaa
     6601 aatcactaaa tcccatttaa taaaggagtt gaccgacgtt ttaagaaact ctagcacctc
     6661 aggatccatg tcgtccatca ggcgagccgc gagatcagtc ccgaccggtc gtttaggagc
     6721 aggattccat tcttgaaaag cactcgattt tggatcgctc atttccgtat ctttactggt
     6781 gtagcggatc gcgtgcccgc gccagccaat tggctggccg agagtggctc cgcgcccggc
     6841 gccccatgac aatgtttgta ttttttaccg ctcccacatg gacaaagatc atttcgaccg
     6901 atcgcatcag gtgacgatgt cttgatcggc ttggccccct ttccgccaga cgctggggca
     6961 ttcgttcgga gttgccgtgc gggtaacgcc gcggcttgcg gcgctgcttg aacccgatag
     7021 atatcccgtg cgacctgaga tcggattgag ctcaacatgc catcatacat ttcagcggct
     7081 tcacgtttat aagccacgag tggattctgt tgtccgaacg cgcgcagccc gatgccttca
     7141 cgcaagttat ccagatcagt cagatgccgc acccaatgcc catcgacggc gcgcagtaaa
     7201 gtcatccgct cggcttgcgg catgagatcg gctagctgct tggtcttttc agcatagcct
     7261 tgctcggcaa attcaatcaa ctgctgctcg atctcttcaa cgctcaggcc gcgccaatcg
     7321 ttgattgtca gcgttggcgg cagcgggatc atcccgcgca tgctgatcat taagccgctt
     7381 aagtcccatt catcttcgtg ctgagcctgg gtgtgctgag cgatgacagc ctgaatttca
     7441 tcatacatca tatcgaggat aatcgggcgc agattcgatt cgttcaatat cttgcggcgc
     7501 tgggcataga tcacaccgcg ctgcgtatta accacatcgt cgtattccag cacatgcttg
     7561 cgcgaatcaa agttgtaacc ttcaacgcgc acttgggctt gctcgatcga tcgagacagc
     7621 agcgaatgct ccagcggttg atcctcatcc atcttgaagc gatccatcaa attcttgatc
     7681 gaatcgccgc cgaaacgccg catcaaatcg tcttcgagcg aaatgaagaa tcggctcgaa
     7741 cccggatcgc cctgacgtcc ggcgcgaccg cgcagctgat tgtcgatccg acgcgcctca
     7801 tggcgttccg tgccgacgat atgcaagccg ccggctttca agacttgctc gcgctcagtc
     7861 gcaaccatgc gttgagcctc ggccagcgct tgatcccagt cggccggtga aatttcggtt
     7921 agatcgactc ctttctttcg caacagatca cgcgcaatac cttccgcgtt gccgcccaac
     7981 aaaatatcga caccgcgacc ggccatattc gtcgcgaccg tcactgcgcc gacccgtcct
     8041 gcctgcgcga tgtagaccgc ttctttctca tgctctttgg cgttcaacac attgtgcgga
     8101 atgcctctgc gcttgagcat tccggccagc atttccgaag tttcaatcgc aaccgtgccg
     8161 atcaacacag gttggccctg cttgttcatc tcctcaatct cactcacaac cgatcggaac
     8221 ttggcatcct ccgttttgaa gatcacatcc ggcgtgtcct cacgaatcat ctcgcgatgc
     8281 gtcggaatcg acaccacatc caagttatag atcttctgga actcttcttc ttctgtcttg
     8341 gccgtgccgg tcatgccggc taatttccgg tacattcgga aaaaattttg aaaggtgatc
     8401 gttgcgtaag tcaagttttc gcgctgaaca gtgacctttt ccttcgcctc gatcgcttga
     8461 tgcagacctt cgctatagcg ccggccgtac atcaatcgcc ccgtgaactc atcgacgatg
     8521 atcacctgtc cgtctttgac gatgtagtct ttatcgcgct tgaagatcac attggcgcgc
     8581 aaggcgttgt ccaaatacgg aatgagatcg gagtgttcgg gcgcatacag attcggaatg
     8641 cctgtcacct gctcgatgcg ctcgatgccc gcttcggtca acgtcgcatt acgcgtcttg
     8701 agatcgacgg tatactcgcc attgggatgg tcttcatcgt cgttggtcgt cgggcgcagc
     8761 ctgcgcacga tctcggagaa acgcacatac tgatcgctcg actcttcagc cgggccggaa
     8821 atgatcagcg gcgtgcgcgc ttcgtcgatg aggatgttat ccacctcgtc gacgatggcg
     8881 tattgcagtt cacgctgagc gcattgagca agttcccaga ccatgttgtc gcgtaagtaa
     8941 tcgaagccga actcattgtt tgtgccgtag gtaatgtcgg cacgataggc atctcgacgc
     9001 gacaccggcc gcaatcggtt atagcgatcg tcctgcgcct tgaaatcagg atcgaacaca
     9061 aaggcggact cgtgctgaat cacgccgact gttgtaccta gcccgtgata gatcggtccc
     9121 atccactgcg cgccgatctt ggataagtaa tcgttcggcg tcaccagatg cccaccatga
     9181 ccggctaacg cgttcagata cagtggcaaa cttgctacca gcgtcttacc ttcaccggtt
     9241 ttcatttcgg agatcttgcc ttgatgcagc accatgccgc cgatcagctg tacatcgaag
     9301 tgacgcaggc cgacattacg cttggccgat tcgcgcacag ccgcaaaagc gcgcggcaga
     9361 aaatcgctga gcagatcatc ttcggtggtg aataattcgt cgtcgagttt cttcacgcgc
     9421 agatcgagct gccgccgtcg
//
LOCUS       DASRTJ010000555         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_109912,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000555 DASRTJ010000000
VERSION     DASRTJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109912"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFF70_11300"
     CDS             <1..746
                     /locus_tag="VFF70_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZY45325.1"
                     /translation="PQLQAAGPLLNTPESTFPVLFGRTMIWTVVNVVFHVLGGMGLAL
                     LLNRPLKLRGLYRTLLIIPWAIPQVIAALAWRGEFNGAYGFINIMLTRLGLVAPPWLQ
                     DPAWAFLAVIIVNVWLGIPFMMVVILGGLQSIAGEYYEAAEIDGASRSQQFRAITLPL
                     LRPVLGPAITLGVIWTFNNFNIIYLVTNGGEPAEQTNILVTALYNAAFGFSRYGFAAM
                     FSLVIFAILFTFAYGWIRYSGALKGVYES"
     gene            743..>1645
                     /locus_tag="VFF70_11305"
     CDS             743..>1645
                     /locus_tag="VFF70_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY45326.1"
                     /translation="MTTDSRSIEIAQAQLTVSDRVRLFFIRPRGDSPFRSLWINALLI
                     MACIIAIYPALRVFTISLRPNDQLFSLSLDIIPKDATLANYSRLFSDKNFLVWIWNSL
                     LVTVFVSVLGVSMAATSAYAFSRFRFRGRNISLLFLLTTQMIPAGMLLIPIYLFLARV
                     GLLDTYKGILLAYVVSSIPFSIWILKGYYDTVPVDLEEAAMIDGASRLVTFLRIILPL
                     STPALAIVFLFNFMQSWQEFPVAYQIVQRKELLWTWPLGIKAMQGQFQTEWGMYAAAS
                     FMIMIPVLALFLYSAKWLISGLTLG"
BASE COUNT          292 a          454 c          433 g          466 t
ORIGIN      
        1 atccacaact gcaagccgcc ggcccattgc tgaacacacc tgaatcgact ttcccggtat
       61 tgtttggccg cacgatgatc tggacggtgg ttaatgttgt ttttcatgtt ttgggcggta
      121 tgggtcttgc gctgttgctg aaccggccct tgaaactgcg cggactctat cggaccttgt
      181 tgattattcc gtgggcgatt ccgcaagtga ttgcggcttt ggcctggcgc ggtgaattca
      241 acggcgccta cggctttatc aatatcatgc tgacacgctt aggtttggtc gctccgccgt
      301 ggctgcaaga tccggcctgg gcttttctag ctgtcatcat cgtcaatgtt tggctgggca
      361 ttccgtttat gatggtggtt attctcggcg gcttgcaaag tatcgccggc gaatattatg
      421 aagctgctga gatcgatggc gcgtcccgct cgcagcaatt tcgggcgatc acgctgccgc
      481 ttctgcggcc ggttttgggc ccggccatta cgctgggcgt catctggacg ttcaataact
      541 ttaacatcat ctatctcgtg accaatggcg gcgagccggc tgagcaaacc aacatcttag
      601 tgacggcgct atacaatgcc gctttcggtt tttcgcgcta cggtttcgcg gcgatgttct
      661 cgctggtcat ctttgcgatt ctctttacct tcgcctatgg ctggattcga tatagtggcg
      721 ccttgaaggg agtatacgaa tcatgacaac cgatagccga tccatcgaaa tcgctcaagc
      781 tcaacttacc gtttccgatc gtgtccgatt gttttttatc cgcccgcgcg gcgatagccc
      841 tttccgctcg ctgtggatca atgcgctgct gatcatggcc tgtatcattg cgatctatcc
      901 ggcgctgcgt gtgtttacga tcagcttgcg cccgaacgat cagctcttct cgctctcgct
      961 cgacatcatc cctaaagatg caaccttagc caactacagc cgtttatttt cagacaaaaa
     1021 cttcctggtc tggatctgga acagcctgtt ggtaacagtc tttgtttcgg tcttgggcgt
     1081 gtcgatggcc gccaccagcg cgtatgcctt ctcgcgcttc cgctttcgcg ggcgcaacat
     1141 cagtttgctc ttcttgctga caacacagat gattccggct ggcatgctgc tcatccctat
     1201 ctatttattc ctggcgcggg tcggcctgct ggatacctac aaaggcattc tgctcgccta
     1261 cgttgtgtcg tcgattccct tcagcatctg gattctcaaa ggctattacg acaccgtgcc
     1321 agtcgatctt gaagaagcgg cgatgatcga cggtgcgtcg cgtctcgtca cgttcctgcg
     1381 catcatcctg ccgctgtcta cgcccgcgct ggcgattgtc tttctgttca acttcatgca
     1441 gtcgtggcag gaatttccgg tggcttatca aatcgtgcag cgcaaagaat tattgtggac
     1501 gtggccgttg ggcatcaaag cgatgcaggg ccaattccaa actgaatggg gcatgtatgc
     1561 cgcggcgtca ttcatgatta tgatcccggt cttagccttg ttcttgtatt cggccaagtg
     1621 gctgatcagc ggcctgacgc tcggc
//
LOCUS       DASRTJ010000556         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_34974,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000556 DASRTJ010000000
VERSION     DASRTJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34974"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /gene="ade"
                     /locus_tag="VFF70_11310"
     CDS             complement(<1..1615)
                     /gene="ade"
                     /locus_tag="VFF70_11310"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HZY45327.1"
                     /translation="MELSQFIKIARGDEPADLLLKGGSIVDVFSGDVYAADVAIADGR
                     IVGVGSNYRAKETIDVSGSVIAPGFIDAHVHIESSLVPPPQFARAVVPRGTTSVVIDP
                     HEIANVLGLDGIRYMFDAAKYNPLSVFVMVPSCVPATNMETSGAVLHSYDLTSFKQDR
                     WVLGLAEMMNFPGVIFGDEEVLAKITEFDGTIRDGHAPGLAGQQLNAYIAAGIGSDHE
                     CTTVDEAREKLRLGMTIFIREATNAHNLEPLLPLINATNARRFCWCTDDRQPSDLLDL
                     GGIDYLIRHSIELGLDPVTAIRLGTLNTAEYFRLHDRGSIAPGKRADLVVLNNLKEVK
                     AKFVLRGGAIVARDGELLAYAKPQRDIFVRSSMNVDLDQIDFRVKATGRKANIIGAIP
                     DQLITEHLIEDLTIENGLAVQDPGLDVLKIAVIERHAHTGAHAIGFVKGIGLRAGAIA
                     SSVAHDHHNIVVIGADDRSMLTAVRAIVQTHGGMSAATGDQVVSLLPLPIAGLMSDQP
                     IEKVRDQMDELVRSAHQLGSTLHDPFMAMS"
     gene            complement(1737..>2817)
                     /locus_tag="VFF70_11315"
     CDS             complement(1737..>2817)
                     /locus_tag="VFF70_11315"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010953507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZY45328.1"
                     /translation="LYPIWARMNAKAVYTSALTGMAELILSGCTTSSDHLYLFPNGSK
                     LDDEIRAAQEIGLRFHAARGSMSLGESKGGLPPDSVVEDEKSILKDCVRVIEQYHDSK
                     PGAMLRIVVAPCSPFSVTENLMRESASLARQYNVQLHTHLAETFDEDDFCKQMFGVLP
                     VEYAERVNWLGDDVWYAHSVHVREQDIDRMAQTQTGAAHCPSSNMRLASGIAPIRSMI
                     DHGVPVGLGVDGSASNDGSHMLNEARQAMLLQRVNGNPSGLTGLEALELATLGGAKVL
                     GRDDIGSIEVGKCADFIGFGLDRLEYAGAQHDPQAALIFCAPQCVELSVINGKIVVED
                     GELRTIDIGKVIENHNKISRKLING"
BASE COUNT          612 a          819 c          762 g          624 t
ORIGIN      
        1 aggacatggc cataaacgga tcgtgcaaag tcgatcccag ctggtgcgcg gagcgcacca
       61 gctcatccat ttgatcccgc actttctcga tcggctgatc ggacatcaag ccggcgatcg
      121 gcagcggcag caaggataca acttgatcac ctgtggccgc gctcatgccg ccgtgtgttt
      181 gcacgatggc gcggacggcg gtgagcatcg atcgatcatc cgcgccgatg acgacgatgt
      241 tgtgatgatc gtgcgccacc gatgaggcga tggcgcctgc ccgcaagccg atgcccttta
      301 caaacccgat tgcgtgtgcg cccgtatggg catgccgttc gatcacggcg atcttcaaca
      361 catcgaggcc ggggtcttgc acggccagcc cgttttcaat cgtcaagtct tcgatgagat
      421 gctcggtgat cagttgatcg ggaatggcgc cgatgatatt cgctttacgg ccggtggcct
      481 tcacgcgaaa atcgatctga tccaaatcca cattcatcga gctgcggaca aagatgtcgc
      541 gctgcggttt ggcgtaagcc agcagctcgc catcgcgcgc gacgatcgct ccgccgcgca
      601 agacaaattt tgctttcact tctttcaagt tattcagcac caccagatca gcgcgtttgc
      661 ccggagcaat cgatccgcga tcgtgcaaac ggaaatattc ggcggtgttg agcgtcccca
      721 gccggatggc cgttaccgga tcgagcccga gttcgatcga gtgacggatt aaataatcga
      781 taccgccgag atcgaggaga tcggatggct gtcggtcatc cgtgcaccag cagaatcgcc
      841 gcgcattcgt ggcgttgatc aacggcagca gcggctcaag attgtgcgcg ttcgttgctt
      901 cgcgaatgaa gatagtcatc cccaatcgaa gtttctcgcg cgcctcatcg accgtcgtac
      961 attcgtgatc cgatccgatg ccagcggcga tatacgcatt cagttgctgg ccggctaggc
     1021 ccggcgcatg tccatcgcgg attgttccat cgaactcggt aatcttcgcc agcacttctt
     1081 catcgccgaa gatgacgccg ggaaaattca tcatctcggc caggcccagc acccagcgat
     1141 cttgtttgaa agatgtgagg tcgtaggagt gcagcactgc gccggatgtt tccatgttcg
     1201 tcgccggcac gcacgatggc accatgacga aaacactgag cggattgtat ttcgctgcgt
     1261 cgaacatgta gcgaatgcca tctaatccca acacgttggc gatctcgtgc ggatcgatca
     1321 cgacgctggt cgtgccgcgg ggtacaacgg cgcgcgcgaa ttgaggcggc ggcaccaggc
     1381 tcgattcaat atgcacatgc gcatcgataa agcccggcgc gatcacactg ccactcacgt
     1441 cgatcgtctc ttttgcccga tagttcgatc cgaccccaac gattcttcca tcggcaatgg
     1501 cgacatcggc ggcgtagaca tcgccggaga aaacatcgac gatcgatccg cctttcaata
     1561 aaagatcggc cggctcatcg ccacgcgcaa ttttgatgaa ttgggagagt tccatgatta
     1621 tcacctatac gtggtgtcat tctgacgcgc gaagcgccga agaatctcta cctcagttat
     1681 ggatcagtgt tgactggaat agagattcct cgctgcgctc ggaatgatcg cgaaaattag
     1741 ccattgatta acttccgcga aatcttgttg tgattctcga tcactttacc aatatcgatc
     1801 gtgcgcagtt caccatcttc gacgacgatc ttaccattga tgaccgatag ttcgacgcac
     1861 tgtggggcgc agaagatcaa ggccgcttgc ggatcatgtt gcgcgcccgc atactctaat
     1921 cgatcgagac cgaagccgat gaagtccgcg cacttgccta cttcgatcga tccgatgtca
     1981 tctcgtccaa gaactttcgc gccgccaagc gtggcaagtt ccaatgcttc gagtccagtc
     2041 aagcccgacg gattgccgtt cacccgttgg agcagcatgg cttgacgcgc ttcattgagc
     2101 atgtgcgatc cgtcattgct ggcgctgcca tcgacgccga gaccgaccgg cacgccatga
     2161 tcgatcatcg atcggatcgg cgcgatgccg gaagcgagcc gcatgttgct cgatgggcaa
     2221 tgcgctgcgc ccgtttgcgt ctgcgccatc cgatcgatat cctgctctcg cacgtggacg
     2281 ctgtgcgcat accagacatc atcgcccaac cagtttactc gttcagcata ctcgacgggc
     2341 aacacaccga acatttgctt acaaaaatca tcctcatcga atgtttcggc cagatgcgta
     2401 tgcaactgca cgttgtattg ccgtgccaac gacgccgatt cgcgcatcaa gttttctgtg
     2461 accgagaaag gcgagcaggg cgctacgacg attcgaagca tcgcgcccgg tttcgaatcg
     2521 tgatactgct caatgacgcg cacgcaatct ttgagaatcg atttttcatc ttcgaccacg
     2581 ctatcaggcg gcaggccgcc tttcgattca ccgagtgaca tcgatccacg cgcggcgtga
     2641 aagcgcaggc caatttcctg cgcggcgcga atctcatcat caagtttcga tccgttggga
     2701 aagaggtaca aatgatcgct ggacgtcgta cagccgctga ggattaattc ggccatgccg
     2761 gttaaggcac tcgtgtaaac tgccttggca ttcatgcgcg cccagatcgg atataag
//
LOCUS       DASRTJ010000557         8184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_280080,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000557 DASRTJ010000000
VERSION     DASRTJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280080"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..805)
                     /locus_tag="VFF70_11320"
     CDS             complement(110..805)
                     /locus_tag="VFF70_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY45329.1"
                     /translation="MAAPFEGRYRSIGYEAIYAARLAIREINANGGINGYRIELVALD
                     DRGDPDQAIEAARKLTIDPQVIAVIGHYRPDSTEAAIGEYCEQQVPVIAIESAAPLPE
                     KCWPPIDAFVLGHASIERWISKPLIFVSNVPDLDGLAAAQSFVSAYNAIPIDGTRAGP
                     IALQTYDAMHMVFEAIRRAAKIDRVSVAAAIASNQFKGLGASYQFDPQGKLIDAQIFI
                     FEYGNDGKPKLIP"
     gene            complement(949..2088)
                     /gene="mltG"
                     /locus_tag="VFF70_11325"
     CDS             complement(949..2088)
                     /gene="mltG"
                     /locus_tag="VFF70_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZY45330.1"
                     /translation="MKKRHRRLILFLFVIVFVCGALGAVGVLIFGKGVNLGGGIQLAA
                     PKDLQQFALSMYLNIRAGDLTAPAGKDKTPIEFTIAPNETIGEVSNQLLSLHLIIDAD
                     LFRRYLQYNGQDAGVEAGKFELNQAMNIPEVAQALQTGKIEEVSVTIPEGKRIEEVAE
                     IVAGQVPITATEFLALAKDPGPWKAQYPFLNELPPKATLEGYLFPDTYSLPKDKVSAH
                     DLINRMLTNFNAKATQQMRADIQTSGRTLFDVIRLASIVEREAVVADERPRIAGVYSN
                     RLTASMALDADPTVQYALGSTREPGHWWPQITQDDYQAVQSPYNLYLNPGLPPGPIAN
                     PGFSSIKAAAYPEKNDFYFFRAACAQDGTHQFAKTIEEQIAHACP"
     gene            complement(2150..2590)
                     /gene="ruvX"
                     /locus_tag="VFF70_11330"
     CDS             complement(2150..2590)
                     /gene="ruvX"
                     /locus_tag="VFF70_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZY45331.1"
                     /translation="MKRLLAIDVGEKRIGVAISDETWTLARPLLTVMRGSKTEDFQKL
                     KTVCDQYQIDKIICGLPKTLRNEIGPQAQRVQRYADELTASIGLPIEFWDERFSSVEA
                     EERLAASSRKKRAKGSIDSAAAAIILQEYLDSMRNDHLTMNNGK"
     gene            complement(2614..4149)
                     /locus_tag="VFF70_11335"
     CDS             complement(2614..4149)
                     /locus_tag="VFF70_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HZY45332.1"
                     /translation="MEQIIELTEHDDVHSIRDRLALAQSPRVLLVLPWDSPLLRHAVD
                     LQIVQRFAAANQIEVGIVSEEAEARLVIRETGLPVFRSIDSAQRKKQWTKPIDEEDEL
                     APWKPSLKRKREAEQAAVERDQMTAQSHQRSPYRHFFKYALVSVVAMALILAACAVIP
                     SARIVLVPRTTQVVANVNVIADPDTSGVDAISAHVQAEEVPLDVRDQVTVPTTGKKDI
                     PQSRATGRVIFVNQLNTPIRVSQGTAVRTSSGSQAVRFILTQDVDVPGGIGAQAEGTL
                     EAVEPGYQGNVPANFINEVEGVASLAVRVTNPEAMTGGGAKEVPSVDPVDRETATAQL
                     TKILRDDAQKQLQAKLGADEFIIPESLSGNILEATFDHEVTEEADQLTLLMRVHYTIL
                     KVSSADANSLVFTAMQHQAPPGYELIPEGLSFQRGTASPVEGSDTWFQFQMQGVGYAA
                     ANLDIAHAAKVITGKPIGTASDTLKELLPLKRDPEITTIPSWFPWIPWLTFRIHTEVN
                     PQG"
     gene            complement(4348..7188)
                     /gene="alaS"
                     /locus_tag="VFF70_11340"
     CDS             complement(4348..7188)
                     /gene="alaS"
                     /locus_tag="VFF70_11340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZY45333.1"
                     /translation="MTKKQDSIKKTTKPKSIKTAAPKSPAKSIGLAQPDRFVSSALIR
                     DQYLQFFKDRGHTIVPSIPLIPVGDNTLLFTNSGMVQFKDVFVGVGTRPYKRVADSQK
                     CMRVAGKHNDLEDVGRDTWHHTFFEMLGNWSFGDYYKKEAITWAWQLLTDMWGLPKDR
                     LWATCFEDELGVIPRDDEAAGYWLKQPGFDPKHLLFFGRKENFWEMADIGPCGPDSEI
                     HYDRGPGFCDKQHIKEHVCHVNGDCGRFLEIWNLVFIQYQRTDPTNLTPLPAMHVDTG
                     MGFDRMVSILQNVDSNYKTDLFMPLIHRTQELAGHTDAEVEENIVAYRVIADHSRAAA
                     FLIADGALPGNVGRNYVLRMILRRAARFGRKIGFDRPFLSLIADVVIENMGVHYRELH
                     DRRDHILYTINDEEKRFFRTLDLGLAKLDELLEKSANKIIPGADAFKLYDTFGLSVEI
                     TKDIALERGYTVDEAGFQAAMAEQREKARSAEKFAVGNESLAIYQQVKQQLIDQGVIG
                     DQGVEHDPYTAPEMEAKLIGLINNGQSIDQAKPGDKVEVILPSTPFYIESGGQISDTG
                     IIAQYSARGGEPTWEIEVTDTRRVLPGLIVHVGMVKSGKPKVGDDCMAIVDLDRRLDI
                     MRNHTATHLLQAQLRSILGQHVQQAGSLVAPDRLRFDFTHGSMLTQDEIDRLSENVNN
                     LILANYPLTTTYRNYKDAVGEGAMALFGEKYGEIVRVVRTGFPNEKPVSMELCGGTHV
                     TQTAEIGSFVIVSEGSVGAGLRRIEAFTGRGAQHYVQSRLKLLNNAAAYLKTTPEEVD
                     RKVLEVIELAQSKEKELSKAQRESAQRDFETLLDQVEDVSGVSVLAAQVNAVDVDMLR
                     EMSDWFRAKLKSGVIVLGAVVDGKPNLLAAVTPDLVERGLDAGKIVRSAAQIIGGGGG
                     GKPTLAQAGGKDASRLSEALSSVKQNIAASIK"
     gene            7499..>8184
                     /gene="tyrS"
                     /locus_tag="VFF70_11345"
     CDS             7499..>8184
                     /gene="tyrS"
                     /locus_tag="VFF70_11345"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZY45334.1"
                     /translation="MPSIDEQVALLMQGTEYGDVQLKQNMARDLKERLLEAQKEKRPL
                     RVYCGFDPRTSDLHLGHTIPMRKLRQFQELGHEVTFLVGNYTSLIGDPSDKDKLRPQL
                     TSDEVAQNARTYAEQSYKILDRTKTRVRYNAEWLSELSFADLIKIASNFTVQQFLQRE
                     NFKLRVERNDPIYLHETFYALMQAYDAVAQNTDVQIGGSDQLFNIVVAGRKLQEAMGQ
                     KPQVAIIMGIL"
BASE COUNT         1870 a         2351 c         2051 g         1912 t
ORIGIN      
        1 gtcattatgg gcgcggcgtt cgtgatctta gccttgatgt ggtatacgtt ttattcccag
       61 aacgaaaata agaaatcgtc atgaaggagg ggcaattcat gaattgcccc tacgggatca
      121 atttcggctt gccatcattc ccgtattcaa agataaatat ctgcgcatcg atcaacttcc
      181 cctgcggatc gaattgatag ctcgccccta accccttgaa ttgattgctg gcaatggcag
      241 ctgccacgct cacccgatcg atcttcgccg ctcgacgaat cgcctcaaac accatgtgca
      301 tcgcatcata cgtctgcaat gcgatcggcc cggcacgcgt gccatcgatg ggaatcgcat
      361 tataagctga gacaaaactt tgggcagcag ctaagccgtc gaggtcgggc acgttgctga
      421 caaagataag cggcttactg atccaccgtt caatcgaagc gtggcctaac acaaaggcat
      481 cgatcggcgg ccaacatttt tccggcagcg gcgcagcact ttcgatcgca atcacgggca
      541 cttgctgttc gcagtattcc ccgatcgccg cttcggtgct atcgggtcga tagtggccga
      601 tgactgcgat cacctgtgga tcgatcgtga gtttgcgcgc cgcctcgatc gcttgatcgg
      661 gatcgccgcg atcgtccagc gccaccaatt cgatccggta accgttgatg ccgccattcg
      721 cattaatctc acgaatcgcc aatcgcgctg cataaatcgc ttcgtaaccg atcgatcgat
      781 agcggccttc gaagggtgcg gccaatccga tcttaatgac gggttgagtg gaaccgggga
      841 agttgcacgc ggagagcccg agaaggagag atagagagac agagagaatc aatgaatgcg
      901 agaaaagttt tttccgctcc atcacgcatt gcctcattcg ctcaatgatc atgggcacgc
      961 gtgcgcgatt tgttcttcga tcgtcttggc gaattgatgc gtgccatctt gcgcacaagc
     1021 cgcgcggaag aaatagaaat cgtttttctc tggataagcc gccgctttaa tcgacgagaa
     1081 accgggattg gcaatcggac cgggcggcag accgggattg agatacaagt tatagggcga
     1141 ctgcaccgct tgataatcgt cctgcgtgat ctgcggccac caatgtcccg gctcacgtgt
     1201 gctgcctaaa gcatattgca cggtcggatc agcatccaac gccatgctgg ccgtcaatcg
     1261 atttgaataa acgcccgcga tgcgcggccg ctcatccgcc acgacggctt cacgctcgac
     1321 gatcgatgca aggcgaatga cgtcaaacaa cgtgcggcct gaagtttgaa tatcggcgcg
     1381 catttgctgg gtagctttag cattgaagtt ggtcaacatg cgattgatta ggtcatgcgc
     1441 ggagactttg tctttcggca aactatatgt atccgggaag agataacctt ccagcgtcgc
     1501 tttaggtggc agttcattca aaaacggata ttgcgctttc cacgggccgg ggtcttttgc
     1561 taaagccaga aattcggtgg ccgtaatcgg aacttgtccc gcgacgatct cggctacttc
     1621 ttcgatgcgc ttgccttccg gaatcgtgac gctcacttct tcgatcttgc ctgtctgcaa
     1681 agcctgcgcg acttccggaa tattcatcgc ctgattcaat tcaaacttgc ccgcttccac
     1741 gccagcgtct tggccgttgt attgcaaata gcggcggaag agatctgcat cgatgatcaa
     1801 gtgcagcgac aataattgat tgctgacttc gccgatcgtt tcattcggtg cgatcgtgaa
     1861 ctcgatcggc gttttgtcct tgccggccgg cgcggtcaga tcgcccgcgc ggatattgag
     1921 atacatcgac agcgcaaact gctgcaagtc tttcggtgca gccagttgaa tgccgccgcc
     1981 cagattcacg cctttaccaa aaatcagaac gccgaccgcg cccagcgcgc cgcagacaaa
     2041 cacaatgacg aacaagaata agattagacg acgatggcgc tttttcattg tagataatta
     2101 gtgaagagta attagtgctt ggcagtaagt gatctggaat ccaaatcgtt catttgccat
     2161 tgttcattgt taagtgatca ttgcgcattg aatcaaggta ttcttgtaga ataatggctg
     2221 ccgccgccga atcgatcgaa cctttggcgc gtttcttgcg gctcgatgcc gctaaccgtt
     2281 cttcagcctc gaccgatgaa aaccgctcgt cccaaaattc gatcggcagt ccgatcgagg
     2341 cagtgagctc atcggcataa cgttggacgc gctgtgcctg cggaccgatc tcattgcgca
     2401 aagttttcgg cagaccgcaa atgattttat cgatctgata ttgatcgcaa accgttttga
     2461 gtttttgaaa atcctcggtc tttgaaccac gcattacagt caatagcggc cgtgccagcg
     2521 tccaggtttc atcgctaata gcgacaccta tccgcttctc gccgacatca atcgctagaa
     2581 gtctcttcat cccaaatcaa aaatcgaacg gacttaacct tgcggattca cttcggtgtg
     2641 aatgcggaac gtcagccacg gtatccacgg aaaccaactg gggatagtgg taatctcagg
     2701 atcacgcttg agcggcagca gctccttcaa cgtatcgctc gccgtgccga tcggtttgcc
     2761 agtaatcacc ttggccgcat gggcaatatc aagattcgcc gcagcgtaac ctacaccctg
     2821 catctggaat tgaaaccacg tatctgagcc ttccaccggg ctggcagttc cgcgctgaaa
     2881 actcaagcct tccggaatca attcatagcc gggcggcgct tgatgctgca tcgcggtgaa
     2941 tactaaactg ttcgcatcgg cgctgctgac cttcaatatc gtataatgta cgcgcatcaa
     3001 cagcgtcaat tgatcggctt cttcggtgac ttcatgatcg aatgtcgcct cgagaatgtt
     3061 gccgctcaac gattcgggga tgatgaactc atcggcaccg agtttcgcct gcagctgctt
     3121 ctgcgcatcg tcgcgcaaaa tctttgtcag ctgggccgtc gcagtttctc gatcgaccgg
     3181 atcgaccgac gggacttctt ttgcgccgcc gcccgtcatc gcctcaggat tggtgacccg
     3241 gacggccaac gacgcaaccc cttctacctc attgataaag ttcgcaggca cattgccttg
     3301 ataacccggt tcaaccgctt cgagtgtgcc ctcggcttgc gcgccgattc cgcccggcac
     3361 atccacatct tgcgtaagaa taaaacgcac cgcttgactg cccgacgagg tgcgcaccgc
     3421 tgtgccttgg ctcacgcgga tcggcgtatt gagttgattc acaaaaatga cccggccggt
     3481 cgcgcgtgat tgcggaatgt ctttcttgcc ggtcgttggc acggtcactt gatctcgcac
     3541 atccaacggc acttcttcag cctgcacatg cgcactgatc gcatccacgc cgctcgtgtc
     3601 cggatcggca atgacattga cgtttgcgac cacctgagtg gtgcgcggca ccaacacgat
     3661 ccgagcactg ggaatcaccg cacaggccgc taagatcaac gccatcgcga cgacactcac
     3721 cagcgcatat ttgaagaagt gccgatacgg cgatcgctgg tgcgattgcg ccgtcatttg
     3781 atcgcgctct acggcggctt gttctgcctc gcgcttccgt ttcagcgatg gcttccatgg
     3841 cgcgagttcg tcttcctcat cgatcggctt ggtccattgc ttcttacgct gggctgaatc
     3901 gatcgatcga aagacaggca ggccggtttc gcgtataacc aatcgagcct cggcctcttc
     3961 ggacacaata cccacttcaa tttgatttgc ggccgcaaag cgttggacga tctgcagatc
     4021 gaccgcatgg cgcagtaggg gcgaatccca aggcagcacc aacaacaccc gcggcgattg
     4081 agccagcgcc aatcgatcgc ggatgctgtg aacgtcgtcg tgttcagtta gttcgatgat
     4141 ctgttccata aaacttcttc accgcagaga acgcagagat cgctgagaaa aacaagttat
     4201 tagtctgcgt tctgtgggtc ctctgcggtt aattcaatcc aaacattatt cgaggcgacc
     4261 ctatactgcg gtcgcctcgc ttgagagtgc ggttatttta ccattaagag tagatagcgc
     4321 aaacagactg cgatttgcgg gtcgaattta cttaatcgaa gcagctatat tctgcttcac
     4381 gctcgataag gcctcactca aacgactcgc gtccttgcca cccgcctgcg cgagcgtggg
     4441 cttgccgccg ccaccgccgc cgatgatctg cgccgccgat cgaacgatct tgcccgcatc
     4501 gagtccgcgt tcgaccagat cgggcgtgac ggctgccagc agattcggtt tgccgtcgac
     4561 caccgcgccg agtacgatca cacccgattt tagtttcgcg cggaaccaat cgctcatctc
     4621 gcgcagcata tccacatcga ccgcgttgac ttgcgcggcc aacaccgaaa cgccggacac
     4681 atcttccact tgatcgagca gcgtttcgaa atcgcgctgg gcggattcgc gctgcgcctt
     4741 actcaactct ttttctttag actgcgccag ttcgatcact tccaatacct tgcgatcgac
     4801 ttcctccggc gtggtcttca aatacgcggc cgcgttgttc aacagtttca agcgcgattg
     4861 aacatagtgc tgcgcaccgc gaccggtaaa tgcttcgata cgccgcaggc ccgcgccgac
     4921 gctgccttct gaaacgatca caaacgatcc aatctccgcc gtctgcgtga cgtgcgtacc
     4981 gccgcacaat tccatgctca ctggcttctc attcggaaag ccggtccgaa ccacgcgcac
     5041 aatctcgcca tatttctcgc caaataacgc catcgcacct tcacccaccg catctttgta
     5101 attgcgatag gtggtcgtca gcggataatt tgcgaggatc aaattgttga cattctcgct
     5161 cagccgatcg atctcatcct gagtcaacat cgatccgtgc gtgaagtcaa agcgcaatcg
     5221 atcgggcgcg accagcgatc cggcttgctg cacatgctgc cctaagatcg atcgcagctg
     5281 cgcttgcagc aaatgcgtcg ccgtgtgatt gcgcatgatg tccaagcgtc gatcgagatc
     5341 gacgatcgcc atacagtcat cgccgacttt cggcttgcct gacttcacca tgcccacatg
     5401 cacgatcaaa cccggcagga cgcggcgcgt atctgtgact tcgatctccc aggtcggttc
     5461 gccgcctcgc gcagaatatt gcgcgatgat gcccgtgtcg ctgatctggc cgcctgattc
     5521 gatgtagaac ggcgtcgaag gcagaatcac ttcgacttta tcgccgggct tggcttgatc
     5581 gatggactgg ccgttgttga tcaacccgat cagtttcgct tccatttccg gcgcagtgta
     5641 gggatcatgc tccacacctt gatcgccgat cacaccttga tcgatcaact gctgcttcac
     5701 ctgttgatag atcgccagcg attcattacc gaccgcaaac ttctccgccg atcgcgcttt
     5761 ctcgcgctgt tcggccatcg ccgcttggaa accggcctca tcgaccgtgt agccgcgttc
     5821 caacgcgatg tctttggtga tctcaacgga caacccaaat gtatcgtaca acttgaacgc
     5881 atccgcgccc ggaatgattt tgttcgccga cttctccaac agctcatcaa gtttcgccaa
     5941 acccaaatcg agcgtgcgga agaaacgctt ctcttcatca ttgatcgtat acagaatgtg
     6001 atcgcgtcga tcgtgcagct cgcgataatg cacgcccata ttctcaatca cgacatccgc
     6061 aatcagcgac aggaatggtc gatcgaaacc gatcttccga ccgaagcgcg cggctctgcg
     6121 caaaatcatt ctcagtacgt agttgcgacc cacattgccc ggcagtgcgc catcggcaat
     6181 caagaacgcc gccgcgcggc tgtgatcggc aatgacgcga tacgccacaa tattttcttc
     6241 gacttcggca tcagtgtggc cggccaattc ctgcgtacga tgaatcaggg gcataaacag
     6301 atcagtcttg taattcgaat cgacgttctg caagatagaa accattcgat cgaaacccat
     6361 gccggtatcg acatgcatcg ccggtaatgg tgtcaaattc gtcgggtcag tgcgttgata
     6421 ttgaatgaag accaaattcc agatttcgag gaagcgcccg caatcgccat tcacatggca
     6481 cacatgctct ttgatgtgct gcttatcgca gaacccaggg ccgcgatcat agtgaatctc
     6541 gctatcgggt ccgcacgggc ctatgtcggc catctcccag aaattctctt tgcgcccgaa
     6601 gaacaataaa tgtttcggat caaagcccgg ctgcttcaac caataaccgg ctgcttcatc
     6661 gtcacgcggg atcacaccta actcatcttc gaagcacgtc gcccacaatc gatctttagg
     6721 caagccccac atatccgtca gcaattgcca ggcccatgtg atcgcttcct tcttatagta
     6781 atcgccgaac gaccaattgc ccaacatctc gaagaatgta tgatgccatg tatcccgacc
     6841 gacatcttcc agatcgttat gcttaccagc aacgcgcatg catttctgcg aatcggccac
     6901 acgcttgtaa ggtcgcgtgc cgacaccgac gaacacatct ttgaattgca ccatgccgga
     6961 gttcgtgaac aacaaagtat tgtcgcccac cgggatcaac gggatcgatg ggacgattgt
     7021 atggccgcga tctttgaaga actgcaaata ttgatcgcgg atcagagcgc tgctgacaaa
     7081 ccgatcgggc tgtgctaagc cgatcgattt agcgggcgac ttcggcgcag ccgtcttgat
     7141 cgatttaggt ttcgtcgttt tcttgattga atcttgtttc ttcgtcattc tatcctcatg
     7201 cattcgaagt tggaggttag actttagaag ttagacgatc gatccaactt ccaacacacc
     7261 tgcactgcac aagcacgcag tgcggtgcaa gtgtccatat tccaatcgcc aaaacagtat
     7321 actatacaaa cggctgatgg atcaaaagcc atcagccgtg tgccaaaatt ttagccgata
     7381 gaagcaaggc tgtcaattcg ctatgctata atagcaaaca ctttgtcatt ctgcgcgaag
     7441 cgaggtctag tggcgctgtg agatttgtgc agggtcggaa tgacctctcg gagaaagtat
     7501 gccttccatc gatgaacaag tcgccctgct gatgcagggc accgaatacg gcgatgtgca
     7561 gctcaaacag aacatggcgc gcgatctcaa ggagcggctg cttgaagcgc aaaaagagaa
     7621 gcgtccgctg cgcgtatatt gcggtttcga tccgcgcacg agcgatctgc atctgggcca
     7681 cacgattccg atgcgcaaac tgcgccagtt ccaggaactc ggccacgagg tcacatttct
     7741 cgtcggcaat tacacgtcgt tgatcggcga tccgtcggat aaagataagc tgcgcccgca
     7801 gctcacttcg gacgaggtcg cacaaaatgc acgcacgtat gccgagcaat cttacaaaat
     7861 tctggatcgc accaaaacgc gcgtgcgtta caacgccgaa tggctgagcg agttgtcatt
     7921 cgccgatttg atcaagatcg catcgaactt cacggtgcag cagtttctcc agcgcgagaa
     7981 tttcaaactc cgcgtggagc gcaatgatcc gatctacctg cacgaaactt tctatgcatt
     8041 gatgcaagcc tacgatgccg tcgctcaaaa caccgatgtg caaattggcg gcagcgatca
     8101 actcttcaac atcgtcgtgg ccggccgcaa attgcaagaa gcgatgggcc aaaagccgca
     8161 ggtggcaatc attatgggca ttct
//
LOCUS       DASRTJ010000558         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_262710,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000558 DASRTJ010000000
VERSION     DASRTJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262710"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VFF70_11350"
     CDS             <1..965
                     /locus_tag="VFF70_11350"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HZY45335.1"
                     /translation="YVRRSRRRFWRSEGDADKQAAYSTLHTVLVTLTKLLAPIIPFVT
                     ETMYQNLVRSIDPRAAESVHHCDWPEVESELLDQTLLDDVALSRSIVALGHSIRASNN
                     IKVRQPLSEVIVVVNRTLHDSINRTKDIIADELNVKTVELAENEADFVTYQLLPDNKK
                     LGPKFGAKFPQVRGTLKDAEAFKVVRAVRAGEHVFLGEFDLAPDEILITPQPREGFAI
                     ASENGVVVAIDTHLSNELLREGLAREIVRRVQDLRKSAGLEISDHITLTYRTSGEVKA
                     SIDHWASYIQSETLADEMIDAEPRGTIESEEIDDVKIVLGIEKK"
     gene            1047..1388
                     /locus_tag="VFF70_11355"
     CDS             1047..1388
                     /locus_tag="VFF70_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZY45336.1"
                     /translation="MPIPYDQLKASLLTERLELTLQLEKLKAIATTEAVGYHTHPADD
                     GSAAFDQARDLSIGINEEHTLQMVEDALKRFGNGTYGLCSVCGREIDAARLEAIPYTP
                     YCLDDAAKLER"
     gene            1509..2042
                     /gene="lspA"
                     /locus_tag="VFF70_11360"
     CDS             1509..2042
                     /gene="lspA"
                     /locus_tag="VFF70_11360"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZY45337.1"
                     /translation="MLRRSTVAILLIALLLITLDQVSKYLVSTLLPINGTWSPFPGED
                     PFFRIIYVYNTGAAFGSFRDLGIVFIPIAFLVIAGILYYSRRVSDNQKLLRVALGFML
                     GGASGNLIDRLLRSGHVIDFLDVGVGSTRWYVSNFADISIVLGVILLGVSMWRDEKKQ
                     QPAAVPAPISTDSNPPE"
     gene            2039..2968
                     /locus_tag="VFF70_11365"
     CDS             2039..2968
                     /locus_tag="VFF70_11365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZY45338.1"
                     /translation="MNVATDRTLTLIVEAGQSGRLDRFVAAHVPDLSRTTVQRLIDDG
                     LITCNGSICDAADKVAVGDQIEVRIPPPTLTDILAEDIPLSIVYEDADIIVIDKPAGL
                     VVHPAAGHDRGTLVNALLKHVPDLKGIGGEIRPGIVHRLDKDTSGLMVVAKHDRALQN
                     LQQQFKARTVKKVYVALVEGRVTPPIGIIDGPIGRSRNHRKKMAVTGEGRSARTRYTS
                     IETFHDPDLTLVEAYPETGRTHQIRVHLAWLKHPLVGDTTYGRKQPLFGLQRQFLHAA
                     SLTFALPATGAERTYRSPLPKDLQIVLDELRNK"
     gene            3057..3869
                     /locus_tag="VFF70_11370"
     CDS             3057..3869
                     /locus_tag="VFF70_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZY45339.1"
                     /translation="MREVTIAVVQMAVKFNDVEANLVAMSEWIHKIATAQKVDLIVFP
                     ELATTGYECGVRFTDHAQRVPGPSSNLIAQRASEFGVHVAFGIAAKEKVESVLFNSAV
                     LVGPDGDTIGEYRKVHLKGEERLAFRAGFKFPILETEIGNIGLMIGWDLAFPEVARSY
                     VLDGADFLIECANWEKPNAMEWRTYLLSRAYENTCFIAGANRIGTDATLSFFGESMIV
                     GPRGQIFAAIDEEIEGYAVAKIDLDEVRRYREELQILQARQPSTYRSIVKQY"
     gene            3917..>4309
                     /locus_tag="VFF70_11375"
     CDS             3917..>4309
                     /locus_tag="VFF70_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HZY45340.1"
                     /translation="MLNGMIDFRSDTVTQPTSEMREAMYKAEVGDDVYGDDPTINLLQ
                     ELAAIKVGKASALFVPSGTMGNLTALLTHCKRGDEIIVGDQAHIFKHEVGSYAALGGL
                     SSYQISNNPDGTLPLDRVEAVIRGSDVHE"
BASE COUNT         1019 a         1042 c         1250 g          998 t
ORIGIN      
        1 ggtatgtgcg ccgatcgcgc cgacgttttt ggcgcagcga aggcgatgcg gacaagcaag
       61 ctgcgtattc gactttgcac accgtgttag tcacactgac aaaattactt gcgccgatca
      121 ttccgtttgt cacagagacg atgtatcaga atctcgtgcg ctcgatcgat ccacgcgccg
      181 cagagtctgt tcatcactgc gactggccgg aagttgagtc ggaattgttg gatcaaacgc
      241 tgcttgacga tgtggccttg agccgatcga tcgttgcact cggccactcg atccgcgctt
      301 cgaacaatat caaagtccgg cagccactca gtgaagtgat cgtcgtggtg aatcgcactt
      361 tgcacgacag catcaatcgc acgaaggata tcattgcgga cgagttgaat gtgaagacgg
      421 ttgagttggc agagaatgaa gccgactttg tcacttatca gttgctgccg gacaataaga
      481 aactgggtcc caagttcggc gccaaattcc cgcaggtgcg cggcactttg aaagatgccg
      541 aggctttcaa agtggtcagg gctgtccgag caggcgagca tgtcttcttg ggcgaattcg
      601 atctcgcgcc ggatgaaatt ttgatcacac cgcagccacg cgaaggcttt gcgatcgcat
      661 ccgagaacgg cgtggtcgtg gcgatcgata cgcatctgtc gaatgaactt ctgcgcgaag
      721 gcctggcgcg tgagatcgtc cgccgtgttc aagatttgcg caagagtgcc ggccttgaga
      781 tcagtgatca catcacgttg acctatcgga cttccggcga agtcaaagca tcgatcgatc
      841 actgggccag ctatatccaa tcggaaacac tggctgacga aatgatcgat gcggagccgc
      901 gtggcacgat cgaatccgaa gagatcgacg acgtgaagat cgtgttaggt atagagaaaa
      961 aataatggtt cgtgcggact atgaaataaa gtagaacgca gcgcgtgcca cactatggta
     1021 gaattgtttt gattgaggaa caatcgatgc caattccgta tgatcaactt aaagcgtcac
     1081 ttctaaccga gcgcctggag ttgacgttgc agttggaaaa actaaaagcc attgccacga
     1141 ctgaagcggt tggctatcac acacatccgg ctgatgacgg cagcgcggcg ttcgatcagg
     1201 cgcgtgatct ctcgatcggc attaacgaag agcatacttt acagatggtc gaggatgcat
     1261 tgaagcgttt cggaaatgga acttatggcc tgtgcagtgt gtgtggccgt gagatcgacg
     1321 cggcgcggct cgaagcaatt ccatacacgc cgtattgttt ggatgatgct gccaaacttg
     1381 agcgttgatg agtcaatgca gtccgtgagt agaattggag tcgaggcttt agccggttga
     1441 tgtcgatatc cgacccggct aaagcctcga ctcccgaata ttgaataaac ttcctgagca
     1501 gaggctacat gttgaggcgg agcaccgttg cgattttatt aatagcgttg ttattgatta
     1561 cgcttgatca ggtgtctaag tatttggtgt caactttatt gcctatcaac ggaacgtggt
     1621 cgccattccc cggcgaagat ccattttttc ggatcatcta cgtgtataac accggtgcgg
     1681 cctttggctc attcagggat cttggcatcg tgttcatacc gatcgccttc ctggttattg
     1741 cgggcattct gtattactcg cggcgtgtga gtgataatca aaaattgctg cgcgtggcat
     1801 tgggattcat gctcggcggt gccagcggta atctgatcga tcgactgttg cgttcaggtc
     1861 acgtgattga tttcctggat gtgggtgtcg gctcgacgcg ttggtatgta tcgaactttg
     1921 ccgacatctc cattgtgctg ggggtgattc tgttaggagt atcgatgtgg cgcgatgaaa
     1981 aaaagcagca gccagccgca gtgcctgcgc cgatttcaac cgattcgaat ccgccggaat
     2041 aaatgtggca accgatcgca ctttgacatt gatcgtagaa gcggggcaga gcggccgcct
     2101 cgatcggttt gtggcggccc acgtgcccga tctgtcgcgc accacggtac agcgtttgat
     2161 cgacgacggc ttaattacgt gcaatggatc gatctgcgat gcggccgata aagtggcggt
     2221 gggcgatcag attgaagtga ggattccacc acccacgctg acggatattc tggctgaaga
     2281 tattccatta tcgatcgttt acgaggatgc cgatattatt gtgatcgata agccagccgg
     2341 cctggtggtg catccagccg ctggtcacga tcgcggaacg ttggtgaatg cactgctcaa
     2401 gcacgtgcct gatctgaagg gcatcggcgg cgaaattcga ccgggcatcg tgcaccgatt
     2461 ggataaagat acatcgggat tgatggttgt cgccaagcac gatcgggcgc tgcaaaatct
     2521 tcagcaacaa ttcaaggcgc ggaccgtcaa gaaagtgtat gtggctttag ttgaagggcg
     2581 cgtcacgccg ccgatcggga tcatcgatgg gccgatcggg cgcagccgga atcatcggaa
     2641 gaagatggcc gtgacaggtg aggggcgatc ggcgcgcacg cgctatacat cgatcgaaac
     2701 tttccacgat ccagatttaa ctttagtgga agcgtatccg gagacaggcc gtacgcatca
     2761 aattcgagtg cacttggcgt ggttgaagca tcctttggtg ggcgatacaa cctatgggcg
     2821 caaacaaccg ctgtttggtc ttcagcgcca gtttctacac gccgcatcat taacttttgc
     2881 gctgccagcg accggtgcag agcgaacata ccgatcgccg ctgccgaagg atttacaaat
     2941 tgtgttagat gagttgcgca acaaataatg ggcagtgcgt gctgtgtggg aaattaaacg
     3001 gagcacccac cacgcaccac gcaatgctca agatacatta gccgaaagga aactccatgc
     3061 gcgaagttac tattgccgtc gttcaaatgg ccgttaagtt caatgatgtc gaggcgaatc
     3121 tcgtcgcgat gtcggaatgg attcacaaaa ttgcgacggc gcaaaaagtt gatctgatcg
     3181 tcttcccgga attagccacg accggttacg aatgcggcgt gcgctttacg gatcacgccc
     3241 agcgtgtgcc cggcccatca tcgaacttga tcgcacaacg cgccagcgaa tttggcgtgc
     3301 atgtggcgtt tggcattgcc gcgaaagaga aagtggaaag tgtgttgttc aactcggccg
     3361 ttttggttgg gccggacggt gacacgatcg gcgaatatcg caaagtccat ttgaaaggcg
     3421 aagagcggct ggccttccgc gcaggattca agttcccgat tttggaaacg gagatcggca
     3481 acatcggctt gatgattggc tgggacttgg cctttcccga agtggcgcga tcgtatgtat
     3541 tggatggggc ggactttttg atcgagtgcg cgaattggga gaagccgaat gcaatggaat
     3601 ggcgaacgta tctgctctcg cgcgcatacg agaatacatg cttcatcgcc ggagcaaatc
     3661 gcatcggcac ggatgcgacg ctgagtttct tcggcgaatc gatgatcgtc gggccgcgcg
     3721 gccaaatctt tgcggcgatc gacgaagaga tcgaaggcta tgccgtcgcc aagatcgatc
     3781 tggacgaagt gcggcgttat cgcgaagagc tgcaaatctt gcaagcccgc caacccagta
     3841 cctatcgatc gatcgtgaag caatattagt ccactgattc gcgcagatgc ttgcccattt
     3901 agctggagta acgaaaatgt tgaatggcat gatcgacttt cgatcagata cggtgactca
     3961 accgacgtca gagatgcgcg aggccatgta caaagccgaa gtcggcgacg atgtgtacgg
     4021 tgacgatccg acgatcaatc tcttgcaaga gttggccgcc ataaaggttg gcaaagcatc
     4081 tgcactgttc gtgccgagcg ggacgatggg caatctaacc gcgttgctca cgcactgcaa
     4141 gcgcggcgat gaaatcattg tcggcgatca ggcacatatt tttaagcacg aagtgggatc
     4201 gtatgctgcg ctgggcggac tcagttctta tcaaatttcc aacaaccccg acggcacact
     4261 gccgctcgat cgagttgaag cagtgattcg tggcagcgat gtgcatgag
//
LOCUS       DASRTJ010000559         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_110163,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000559 DASRTJ010000000
VERSION     DASRTJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110163"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1952)
                     /locus_tag="VFF70_11380"
     CDS             complement(<1..>1952)
                     /locus_tag="VFF70_11380"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZY45341.1"
                     /translation="PLYIRAYISEKTHPLLAPLIPEISDLLKEYQIAANGKLAADVVD
                     PINDPSIEAEANQTYGIQSSPLQISDKSGESVINVYFSILIRYGDQDAVLGFRDLIEV
                     TQNADGSPDVHLRNLEYDLTRSIKKVIYGFQSIDAVLSALKDPVKLQVVMTPSSVPDQ
                     LKSAATTIATVAANIQKNSNGKFSFEIVNPDDPKSSITRQTLIDTYKLQPIPVSFFSD
                     QSYYLDLLLTINGQTQVLSVATDYSEASVRTAIESGLKRGTSGFLKVVGLWTPPPSPA
                     AQFGGQPDISSWQAVQQQLGQDYTLTPVDLSGGQVPSNVDVMVVIAPQNMSDKERFAI
                     DQYLMRGGAVIAAAGNFVIAPDQQGGLGLQPVQNGMQAMLDFYGVHIDPKLVMDPQNE
                     PFPVQVSRVVGGLPVQEIQAVNFPFFADIRPAQMDRTSPIVSNLPFVTMNWVSPITLD
                     AAKNANRKTSVLLQSTAKSWLRTSTAIQPNLQLYPDTGYLVEGQRQSFPLAVSVQGSF
                     ESFFKGKPSPFNTNQTPPGQLNPSSIAEPTATPEPAPNFSAIDQSPDTARLVVVGSAE
                     FIDDTVLQLSARLTQDRSAANLQFMQNAVDWSVEDLDLLSIRSRGTITRVLNPMDPNE
                     QSTWEVANYAITLLALIGLGLVWYYRR"
BASE COUNT          442 a          518 c          524 g          468 t
ORIGIN      
        1 cgtcggtaat accacaccag acccagtccg atcaacgcca acagtgtgat cgcataattc
       61 gccacttccc atgtcgattg ctcatttgga tccatcggat ttaagacgcg cgtgatcgtt
      121 ccgcgcgatc ggatgctgag cagatccaaa tcctccaccg accaatcgac ggcattctgc
      181 atgaactgca aattggcggc agaccgatct tgcgttagcc gcgccgacaa ctgcagcaca
      241 gtatcatcga taaactccgc gctgccgaca acgacgaggc gcgctgtatc cggagattga
      301 tcgatcgcgc tgaagttcgg cgcgggttcg ggtgtggccg tcggttcagc tatactcgat
      361 ggattgagct ggcccggcgg agtttgattc gtgttgaacg gtgaaggctt gcccttgaag
      421 aaactttcga aactaccctg cactgaaaca gctaacggaa aagattggcg ttgaccttca
      481 accaagtagc ccgtatcggg atacagttgc agattcggtt gaatagctgt gctggtgcgc
      541 aaccatgatt ttgcagtaga ttgcagcagc accgaggtct tgcgattcgc attcttggct
      601 gcatcgagcg tgatcggtga tacccagttc attgtcacaa agggcaagtt cgaaacgatc
      661 gggctagtgc gatccatctg cgcgggccgt atatcagcaa agaacgggaa gttgaccgct
      721 tgaatttcct ggactggcaa tccgccgacg acgcgcgaaa cttggaccgg gaatggttca
      781 ttctgcggat ccatcaccag tttgggatcg atgtgcacgc cgtagaaatc gagcatcgcc
      841 tgcataccgt tttgcacggg ctgcaaaccc aatccgccct gttgatccgg cgcgatgaca
      901 aagttgcccg cggcggcgat cacagcaccg ccacgcatga gatattgatc aatcgcgaag
      961 cgttccttgt cgctcatatt ttgcggcgcg atcaccacca tgacatccac gttcgatgga
     1021 acttgcccgc ccgatagatc aactggcgtt agcgtgtagt cttgaccgag ctgctgctgt
     1081 accgcttgcc aggacgaaat gtctggctgt ccgccgaatt gtgcggctgg cgatggcggc
     1141 ggtgtccaca agcctaccac tttcagaaag ccggatgtgc cgcgctttag tccagactcg
     1201 atcgccgtcc tgactgaagc ttcgctgtaa tcggttgcaa ccgacagcac ttgcgtctgg
     1261 ccgttaatcg tcaacaaaag atcaaggtag taactttgat cgctaaagaa cgacaccggg
     1321 atcggctgca atttgtaagt gtcaatcaga gtttggcgcg tgattgaact cttgggatca
     1381 tccgggttaa cgatctcgaa actgaatttg ccgtttgagt tcttttgaat attggcggct
     1441 acggtcgcaa tggttgtcgc cgcgctcttc aactgatccg gcactgagct gggtgtcatg
     1501 accacttgta atttgaccgg gtctttcagc gctgacaaga ctgcatcaat actctggaat
     1561 ccatagatca ctttcttgat cgatcgggtc aaatcgtatt ccaggttgcg cagatgcaca
     1621 tccggtgagc cgtcggcgtt ctgcgtcact tcgatcaaat cgcggaaacc aaggactgcg
     1681 tcttgatcgc cgtaccggat caaaatactg aagtacacat tgatcaccga ctcgcccgac
     1741 ttgtcagaaa tttgcagcgg cgatgactgg atgccatacg tttgattagc ctcagcctcg
     1801 atgctcggat cattgatcgg atcgacgaca tccgccgcaa gtttgccatt cgctgcgatc
     1861 tgatactctt tgagtaggtc ggagatttcg gggatcaacg gcgcgagcaa gggatgtgtc
     1921 ttctcgctga tatacgctcg aatatataac gg
//
LOCUS       DASRTJ010000560         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_280183,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000560 DASRTJ010000000
VERSION     DASRTJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280183"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1344
                     /locus_tag="VFF70_11385"
     CDS             157..1344
                     /locus_tag="VFF70_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZY45342.1"
                     /translation="MDGSGLQLRLERYQRLLTISQILNSTLDLKELLDQIVRAAKDLT
                     HSEATSILLVDQKMGDLFFEAASGSQSDAVKRESVPPDSVAGWVVRHGQAQIITDVSQ
                     DQRFSKKTDQNTGFLTRSLLAVPMKIKDRVIGVVEAVNRIGVTPFNEDDVEILTILSS
                     EAAIAIENARLFQQSDLISEMVHELRSPLTSIIAYSELLLRHDLPAEQINSFIETIHH
                     EANRLAKMTNDFLDLSRLQSGRTHLSLTPFDIAALAREVIGLLTPQAAERGITIDGQF
                     SPGPITITGDRDRLRQVLVNLTGNAIKYNRPNGSIEIKIEPQADRIKIEVSDTGTGIP
                     AKDLPHIFEKFFRGPDSEGYAQGTGLGLSIVKQIIEAHNSEIEVRSTPDVGTTFSFML
                     KAA"
     gene            1429..1500
                     /locus_tag="VFF70_11390"
     tRNA            1429..1500
                     /locus_tag="VFF70_11390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1461..1463,aa:Thr,seq:cgt)
     gene            1538..3028
                     /locus_tag="VFF70_11395"
     CDS             1538..3028
                     /locus_tag="VFF70_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45343.1"
                     /translation="MTPSPTLIRDPWRRSWHFLSGDIFLAIALSLSALLLIAAARLPQ
                     TPQNDPIAYSRWLSDTQQRFGGLNGLFTSLGLFSIFNSLVFRLGLGGLGLNAALRLID
                     QIEQLRARDVLPDRPARPAIDHTLDLDLDHIRLKLRGYRVRSHADLLVADHLPRSALA
                     SITLYGAILIVLAGLLIGSFADYRFDNLNVDLPQTTAVSGTPYAIRLDSLNETFDQAT
                     VTLLQQDTSLLQSTLSSSAPAIRSNPAVYLDRIGPALIISAADEAGKPIGLQTTADTP
                     AQPEKSISFNRDHPEGFIAAPDAGLVLQINPVAETIETDYAIQAYQTASGKVLTNTTL
                     PANATLIVNQTIFNFHPSAFITVSLVSQPSHYVICFGLLLVLLGLIGQMIWRPRHLWL
                     HTEADHSRLISDDPTFDPAQLGSMWHATHPSLWIVFAVWIIATVLVSVLTIIAYQRSA
                     SIASISPALIGLWLAFSGSLIANRRTRIVLIVIGVLATFIALFYLI"
     gene            3093..>3238
                     /locus_tag="VFF70_11400"
     CDS             3093..>3238
                     /locus_tag="VFF70_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HZY45344.1"
                     /translation="MTNRPISIGVAGGTGSGKTTIAQEVLKRVGADRIAYIQHDSYYF
                     DKGHL"
BASE COUNT          711 a          955 c          814 g          758 t
ORIGIN      
        1 cccacatgcg tcacgcaaaa ttgatcatca ttaatcgcga gccaacttat gcggataatc
       61 tcggcgaggt ggtcatgcac gccgacgtgg ccgatgtgct gccgcagatt acagccgcga
      121 tccgggcgca ggtttaggat cagatcgagg aagaccatgg atggctccgg attgcaatta
      181 aggctggaac gctaccagcg gctgttgacg atcagccaga ttctcaactc cacactcgat
      241 ctgaaagagc tgctggatca aattgtccgg gcagcgaagg atctgacaca ctccgaagcc
      301 acttcgatct tgctggtcga tcaaaagatg ggcgatcttt tcttcgaagc ggccagcggc
      361 agccagagcg atgccgttaa gcgcgaaagc gtaccgccgg atagtgtggc cggctgggtt
      421 gtgcgccacg gacaagccca gatcatcacg gatgtgtcgc aggatcagcg cttctcaaag
      481 aagaccgatc agaataccgg ctttctgacc cgatcgcttt tagccgttcc tatgaaaatt
      541 aaggatcgcg tcatcggtgt cgttgaagcc gtgaaccgca tcggcgtcac accctttaat
      601 gaagacgacg tggaaatcct aacgatactc agcagcgaag ctgccatcgc cattgagaac
      661 gcacgcttgt tccagcagag cgatttgatc tctgaaatgg tgcacgaact gcgctcgccg
      721 ctgaccagta tcatcgccta tagcgaattg ctgctgcgcc acgacctgcc cgccgaacaa
      781 atcaacagct ttattgagac catccatcat gaagccaatc ggctcgcgaa gatgacaaat
      841 gactttctcg atcttagccg cttgcaatcg ggccgcacac atctgagcct gacccctttc
      901 gacatcgccg cactggcgcg cgaggtcatt ggcctgctca cgccgcaagc cgccgaacgc
      961 ggcatcacga tcgatggaca attttccccc ggtccgataa cgattaccgg cgatcgcgat
     1021 cggctgcggc aagtgctggt taatctgacc ggcaacgcaa ttaaatacaa ccggcctaac
     1081 ggctcgatcg agatcaagat cgaaccgcag gcggatcgga tcaaaatcga agtcagcgat
     1141 accggcaccg gcattcctgc aaaagatctg ccgcatatct ttgaaaagtt tttccgtgga
     1201 ccggacagtg aaggttacgc gcagggcacc ggtctgggcc tcagcatcgt caagcagatc
     1261 atcgaagcgc ataatagcga gatcgaagtt cgcagtacgc ccgacgtcgg cacgacgttt
     1321 agtttcatgt tgaaagcggc ttaagccttt agccattaat tttctcttgt cagttcagct
     1381 gcccgctggt ataatcgacg acgacctccg atggttcaga ttatgcacgc caccctagct
     1441 caatggtaga gcaaccgctt cgtaagtggt aggttgtcgg ttcaagcccg atgggtggct
     1501 cacattggcc ccgctgctgg ggctttttta ttgaagcatg acaccctcac ccactctcat
     1561 ccgcgatccg tggcgccgca gctggcactt ccttagcggc gatatttttc tggcaatcgc
     1621 tctcagcctc agcgccttgt tgctcatcgc ggctgcgcgc ctgccgcaaa ctccgcagaa
     1681 tgatcccatc gcctattcgc gctggctgtc agacacgcag caacgcttcg gcggtttgaa
     1741 cggactgttc acctcgttgg gcttattctc catcttcaac tcgctggtgt ttcgattagg
     1801 gttaggcggc ttaggcttaa acgctgccct gcgcttgatc gatcagatcg aacaactccg
     1861 cgcgcgcgat gtcttacccg atcggcctgc gcgcccggcg atcgatcaca cactcgatct
     1921 tgatctcgat cacatccgat tgaagctgcg cggctaccga gttcgatcgc acgctgatct
     1981 actcgtggct gatcacttac cccgatcagc actggcctcg atcacgttgt acggcgctat
     2041 attgatcgtg ctggctggct tgctgatcgg ttcgttcgcc gattatcgct tcgacaatct
     2101 caacgtcgat ttgcctcaaa cgactgccgt cagcggcaca ccgtatgcca ttaggctcga
     2161 ctcgttgaat gaaactttcg atcaagccac cgttactctt ctacaacaag acacgtctct
     2221 tctacaaagc acgctaagca gttcggcccc ggcgatccgc tcaaacccgg cggtttatct
     2281 cgatcggatt ggtccggcct tgatcatttc tgcagccgat gaggccggca aaccgatcgg
     2341 cttacagacc accgccgata ctccagccca gccggaaaaa tcgatctcat ttaatcgcga
     2401 tcatccagaa ggcttcatcg ctgcgccaga tgccggcctc gtgctgcaaa tcaacccggt
     2461 cgctgaaacg atcgagactg attatgctat ccaagcctat caaactgctt ctggaaaagt
     2521 tttgacaaat accacactcc cagcgaacgc aacgctgatc gtgaatcaaa caatcttcaa
     2581 ctttcatccg tccgccttca tcaccgtttc gctggtcagc caaccgtcgc attacgtgat
     2641 ctgtttcggg ctcttgcttg tactgctcgg tctcatcggc caaatgattt ggcggccacg
     2701 gcatctttgg ctgcacaccg aagctgatca ctcgcgcctg atcagcgacg atccgacttt
     2761 cgatccggcg cagctcggct cgatgtggca tgcgactcac ccctcgctct ggatcgtttt
     2821 tgcggtatgg atcatcgcaa cagtcttagt cagtgtgctt acaataattg cctatcaacg
     2881 cagcgcgtcg atcgcttcga tcagtccggc tttgatcggt ttatggctgg cgttctctgg
     2941 tagtttgatc gccaatcggc ggacacggat cgttttgatc gtaatcggtg tgctggccac
     3001 atttatcgca ttgttctatt tgatttagaa cactcacttc tgactcctaa cttctgccga
     3061 ccacttcttc gcattgaggt ataatcgccg cgatgacgaa ccgacctatt tcgatcggcg
     3121 tggcgggcgg caccggctcc ggcaaaacga cgatcgctca agaagttttg aagcgcgtgg
     3181 gggccgatcg gattgcatac atccagcacg attcgtatta cttcgacaag ggccacct
//
LOCUS       DASRTJ010000561         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_35307,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000561 DASRTJ010000000
VERSION     DASRTJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35307"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFF70_11405"
     CDS             <1..837
                     /locus_tag="VFF70_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45345.1"
                     /translation="MPIVSRSDLNQLDAEVSDMREQLARLEQERADLLMEVHDFELLY
                     HARVDPVHAHLEEVQLHIAEYKLRIDLVRFRGRSLAPSQLEAEVEEQLRVQRQRAEAI
                     GAEAQAAQIEWASIPHDRRDPLLDLDLKQVYRELAKRVHPDLATDTDERITRSQRMAQ
                     VNDLYANHQLNELRRMLREIEIEHLQRNESVEARWARLKREQSQLTAAIYRVKAEIAE
                     LNRSSMPQLKIEFALQKARGHDMLDEVMAQIEAQLHLSEEELNRLIATFREQIESTGL
                     AD"
     gene            951..3533
                     /gene="gyrA"
                     /locus_tag="VFF70_11410"
     CDS             951..3533
                     /gene="gyrA"
                     /locus_tag="VFF70_11410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZY45346.1"
                     /translation="MAARSKAKSKALDTSTHFGNVRQIDIDTEMQQAYLDYAMSVIVA
                     RALPDVRDGLKPVHRRILYAMHDMGLQPNTAYKKSARIVGEVLGKYHPHGDAAVYDAM
                     SRMAQDFSLRYLLVDGQGNFGSVDGDAPAAMRYTEARLASMAAQLLLDIEKNTVEFGA
                     NFDGSLKEPLVLPSAIPNLLLSGASGIAVGMATNIPPHNLSEVCDALVYLIGRWDKID
                     RVTVDELMMRIKGPDFPTGGIVFRYNESVEGGDAIRAAYATGRGRLTIQAKAFIEEAE
                     RGKSRIIVTELPYQVNKSSLIERIADLVRDGKLEGITDLRDESDQSGMRIIIELSRTV
                     EDPRKMLALLYKYTPMQSTFGIINLALVEGEPRLLPLKRMLQLFVEHRQVIIRRRSEF
                     DLTRAKERAHILEGLLKALANLDAVIRLIRSSADADAAKLGLMKRFKLSDVQATAILD
                     MPLRRLARLEREKLDAEYKEKKALIKHLEDLLKDPKKILGVIKNETLVLKEKFGDARR
                     TQIVEKKGKEAEVVTSHDLVTSESAWISIYQDGSVFRMPIGPAILEGKAPAAFVHADS
                     RDTIIVVTERGQAGAIAAHQISSQGMPVAEVFGVPSDQRVVAVFALPKEVPDLPGFLF
                     MTTRQGVVKRVTLIDLAQAFGSFNVMNVADDDAIVAARVTPGEAEMILVTANGQSIRF
                     SEEDVRAMGFNAGGVAGVKLADKDQVIGADVAQARSDLLLLTQNGSGKRSALANFPKQ
                     GRAGQGVVAIKLATGDQVAGVAVVQAADQVVIVTSRGKAKLFRARSAKNAARAAAGSS
                     AYHLTKTEKIVAVIKPAERVVGLVVQEETKPQEKPATTKERKPAAKPKAVKRSPITAK
                     RKSK"
     gene            complement(3653..4033)
                     /locus_tag="VFF70_11415"
     CDS             complement(3653..4033)
                     /locus_tag="VFF70_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45347.1"
                     /translation="MTVTILTVDDEPSSVNLFKLMISIEKPDWVFHGAYNGQEALAIV
                     ANNQVDLVILDIMMPGMDGIEVCRRLRAEPKTAKIPIAMFTALDRPDVRQQAMEVGAT
                     EFWVKPLPPSKLVGAIDRLLAEYK"
BASE COUNT         1083 a         1060 c         1268 g          975 t
ORIGIN      
        1 atgccgattg tttctcgcag cgatctcaat caacttgacg ccgaagtctc ggatatgcgc
       61 gaacagctgg cgcggctcga gcaagagcgt gccgatctct tgatggaagt tcacgacttc
      121 gagttgctgt atcatgcccg cgtggaccca gttcatgcgc acctcgaaga agtccagctg
      181 catattgccg aatacaaact gcggatcgat ctggtccgct tccggggccg atcgctggca
      241 ccgtcacaac ttgaagcgga agtcgaagaa cagttgcgcg ttcaacgcca gcgtgctgaa
      301 gcgataggcg cggaagccca agccgcccag attgaatggg cttcgatacc acacgatcgg
      361 cgcgatcctt tactcgatct tgatctcaag caggtttatc gtgaactagc caagcgtgtg
      421 catcccgatt tggcgacaga tacggatgaa cggataacgc gcagtcagcg gatggcgcaa
      481 gtgaacgact tatatgccaa tcatcagcta aacgaattac gtcggatgct gcgcgagatc
      541 gagattgagc acttacagcg gaatgagagc gtcgaagctc gttgggcgcg actgaagcgc
      601 gagcaatcgc agttgacggc tgcgatctat cgggtgaagg ctgagatcgc cgagctgaat
      661 cgcagctcca tgccgcaatt gaaaatcgaa tttgcgttgc aaaaagcacg cggccacgat
      721 atgctcgacg aagtcatggc gcagatcgag gcgcagctgc acctatcaga agaagagttg
      781 aaccgcctga tcgcaacatt tcgtgagcag attgaatcaa caggtttggc tgattaactt
      841 tttacgttga caacttaatc atgctcggtt aagatgctca tccgataata gtggtcggca
      901 tcttgttttg tttgaaaagt tgtccacttg tcttacaacg gagaaattgt atggcggcta
      961 gatcaaaggc aaaatcgaaa gcactcgaca ccagcacaca ctttggcaat gtgcggcaga
     1021 tcgatatcga taccgagatg cagcaggcgt atctcgatta cgcgatgagc gtgatcgttg
     1081 cgcgcgcgct gccggatgtg cgtgatggtt taaagccggt ccaccggcga attttatacg
     1141 cgatgcatga tatgggcttg cagccgaaca cagcctacaa aaaatccgcc cgtatcgtcg
     1201 gcgaggtgtt gggcaaatat catccgcacg gtgacgcggc cgtgtacgat gcgatgtcgc
     1261 gcatggcaca ggacttttcg ctgcgctact tattggttga tggtcaaggc aacttcggtt
     1321 cggtcgacgg tgacgcaccg gccgccatgc gttataccga agcccgcttg gcttcgatgg
     1381 cggcgcaatt gttattggac atcgaaaaga acacggttga attcggcgcg aacttcgatg
     1441 gttcactcaa agagccgttg gtgttgccgt ccgcaattcc gaacttattg ctgagtggcg
     1501 cgtccggcat tgcggtggga atggcgacga acattccacc gcacaatctg agtgaagtct
     1561 gtgatgcgtt ggtctacttg atcggacgct gggacaagat cgatcgggtg accgttgatg
     1621 aattgatgat gcgtattaaa gggccggatt tcccgacagg cggcatcgtc tttcgctata
     1681 acgaatcggt cgagggcggc gatgcgatcc gcgcggcgta tgccaccggg cgcggccgcc
     1741 tgacgattca agcgaaagcc ttcatcgaag aggcggagcg cggcaagagc cgcatcatcg
     1801 tgacggagct gccgtatcaa gtgaataaaa gcagcttgat cgaacggatt gcggatctgg
     1861 tgcgcgacgg caaattggaa ggcatcaccg atttgcggga cgagagcgac caatcgggta
     1921 tgcggattat catcgagctc agccgcacgg tcgaagatcc tcgcaagatg ttggcgctgc
     1981 tgtacaaata cacgccgatg caatcgactt ttggcatcat caatctggcg ctggtcgaag
     2041 gcgagccgcg tttactgccg ctaaagcgca tgctgcaatt gttcgtcgag catcggcagg
     2101 tgatcatccg gcggcgaagc gaattcgacc tgacgcgagc gaaagaacgc gcgcatattc
     2161 tggaaggtct gctcaaggcg ctggcgaatc tcgatgcggt gattcgcttg atcagatcga
     2221 gtgccgatgc ggatgccgcg aagttgggct tgatgaagcg attcaaattg agcgatgtgc
     2281 aggcgacggc gatactcgac atgccgctgc gccgcctggc gcggcttgag cgtgaaaaac
     2341 ttgacgccga gtacaaagaa aagaaagcgt tgatcaagca tctggaagat ttgttgaaag
     2401 acccgaaaaa gattttagga gttatcaaga acgaaacgtt ggtactgaaa gaaaaattcg
     2461 gcgatgcgcg gcgcacccag attgttgaaa agaaaggcaa agaggccgaa gtagttacca
     2521 gtcacgatct ggtcacgagc gaatcggcct ggatttcgat ctatcaagac ggctcggtgt
     2581 ttcgcatgcc gatcggtccg gcgattctgg aaggcaaagc cccggctgcg tttgtgcatg
     2641 ccgattcgcg cgacaccatc atcgtcgtaa cggagcgcgg tcaagcgggt gctattgccg
     2701 cgcatcaaat ttcaagccaa ggcatgccgg tggccgaagt ctttggtgtg ccgagcgatc
     2761 agcgcgtggt ggcggtcttc gctttgccaa aagaagtgcc tgaccttccg ggtttcttat
     2821 tcatgacgac gcggcaaggt gtcgttaaac gtgtgactct gatcgatctg gcgcaagcct
     2881 ttggatcgtt caacgtgatg aatgtcgccg atgatgatgc gatcgttgca gcgcgcgtga
     2941 caccgggcga agccgaaatg atcttggtta cggcgaacgg ccaatcgatt cgcttcagtg
     3001 aagaagatgt ccgtgcgatg gggttcaatg cgggcggtgt agctggcgtt aaattagccg
     3061 ataaggatca agtaatcggt gcggacgtcg ctcaagcccg atcggatctg ctgctcttaa
     3121 cgcaaaacgg atccggtaag cgatcagcgt tggctaactt cccgaagcag ggtcgcgcgg
     3181 ggcagggcgt agtcgcgatc aagctcgcaa ctggcgatca agttgctggt gtggcagtcg
     3241 tgcaagcggc tgatcaagtg gtaatcgtca cgtcacgcgg taaagcaaaa ttgttccgcg
     3301 cccgatcagc gaagaatgct gcacgggcgg cggctggatc gtctgcctat catttgacga
     3361 agaccgagaa gattgtcgcg gtgatcaagc ccgctgagcg agtagtgggg ctggtcgttc
     3421 aagaagagac caagccgcaa gaaaaaccag ccaccacaaa agagcgcaag ccagctgcga
     3481 agccaaaggc agtcaagagg agtccgatca cagccaagcg gaaatccaag taatacaatc
     3541 acgttgcgtg ttgtcatacc cgcttctgcg ggggtgactg tgtatagcat cctttagaaa
     3601 caaaagtggc gcactcaaca gagtgcgcag caaaattttg taatcgatgc ctttatttgt
     3661 actcagccag aagtcgatct atggcaccta ctaacttgct gggcggcaaa ggtttaaccc
     3721 aaaattcagt tgcgccgacc tccatagcct gttgccgcac gtcgggtcga tcaagggcgg
     3781 taaacatggc gatcggaatt ttcgctgtct ttggctcggc tcgcaaacgg cggcagactt
     3841 caatgccatc catgcccggc atcatgatat ccaaaatcac tagatcgact tggttgtttg
     3901 cgacaatggc caaagcctct tggccgttgt aggcaccatg gaaaacccaa tcgggctttt
     3961 cgatggagat cattaatttg aacagattca cactggaggg ttcgtcatcg acggtaagaa
     4021 tggtaacagt cattatgagg tatattttac tctaataatt ggagttcgtc taagccattg
     4081 tcagcctcat atctattttt ttactgagca acacgttagt tccttgggcc ggtgaattga
     4141 tcgccgggag gttaatcgga tggcagaagc actcaagatc gatctgatcc acttgccatc
     4201 aggcgctgca cggatcatac gccgggaaaa tatcggacac tgatcaagtt gaacttttga
     4261 tgttatcttg cagtatatga tcgtgctatc agccaacttt aaaaccaagg gatagcttga
     4321 cacgtagagt ataataaatc cgtaaggtat gagatatatt attgggtaag ctggatagtc
     4381 caagaa
//
LOCUS       DASRTJ010000562         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_263024,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000562 DASRTJ010000000
VERSION     DASRTJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263024"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VFF70_11420"
     CDS             <1..1251
                     /locus_tag="VFF70_11420"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45348.1"
                     /translation="TLIVGVDAHGKPIGRLYPEDVEIALRMAETHCRPLIHTGWEQID
                     LMGTAVIAVRVARSSELHSLADGRVLIRIGADNMPLGGDAIRQLAATKSSGDFELEAL
                     PGATRSDLDEDVINEYIEKREERQRKRIAMPVDEVLRDLGALDLNGRPTVAGMLLFGR
                     APQQFVPQSGLVFVKFIGTEPRGEGGLPGYGRREEINGPLARVIESAWEVVWEEMHVG
                     AVVKGLEREERTEYPPFAVREALVNSVCHRDYRLKGRRIEIRMFADRMEVISPGGLPG
                     FITVDNLVEEHFSRNPRLVAGLFHWGYIEELGLGIDRMIEEMTLGGHAPPHFKATPYS
                     FTVTLSNIKERKAIPVWEKAMNERQAHALTYLREHGQITNREYQQICPDVSAETLRLD
                     LSDLVDRGALMRIGAKKGTYYILK"
     gene            1394..1795
                     /locus_tag="VFF70_11425"
     CDS             1394..1795
                     /locus_tag="VFF70_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45349.1"
                     /translation="MRYRYPAVYRVQALFGALLAGIILMGSVIAEIESANLPDAFLHA
                     FGFIVSLMILILGLDFGTRTIEITAAGLQTRWLRKSFIAWSHILDWSYRPLGLIHIRL
                     NRGLGLYIWPLMDGYTEILAAIDQHRDQLTT"
     gene            1897..>2099
                     /locus_tag="VFF70_11430"
     CDS             1897..>2099
                     /locus_tag="VFF70_11430"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HZY45350.1"
                     /translation="MPKSNSIHSNPEQKTLMVKWKDGSISEIGWKILRDNCPCAECQE
                     LHGPADPLKLKLAPNYNMVSVDYV"
BASE COUNT          439 a          508 c          659 g          493 t
ORIGIN      
        1 acgttgatcg tcggcgtcga tgcgcacggt aaaccgatcg ggcggctcta tcccgaagat
       61 gtggagatcg cgctgcgcat ggcggagact cactgccgtc cgctgatcca cactggctgg
      121 gaacagatcg atctaatggg cacggcggtg atcgccgtgc gcgtggcgcg cagttcggaa
      181 ctgcattcac tggccgatgg ccgcgtgttg attcggatcg gcgcggacaa tatgccgctg
      241 ggcggcgatg caattcggca actcgcggcg acgaagagca gcggcgattt tgaattagag
      301 gcgctgccgg gcgccactcg atctgatctg gatgaagatg tgatcaatga atatattgaa
      361 aagcgtgaag agcggcagcg caaacgtatt gcgatgccgg tcgatgaggt gctgcgcgat
      421 ttaggcgcgc tggatctgaa tgggcggccg acggtcgcgg gtatgttgtt gttcgggcgc
      481 gcgccacagc aatttgtgcc gcagtcgggt ttggtgtttg taaaatttat cggcaccgag
      541 ccacgcggtg agggcggtct gccgggttat ggccggcgtg aagagatcaa cgggccgctc
      601 gcgcgtgtga tcgaaagcgc ctgggaggtg gtctgggaag agatgcatgt cggcgcggtg
      661 gtgaagggtc ttgagcgcga agagcgcacc gagtatccgc cgtttgctgt gcgcgaagcg
      721 ctggtcaatt cagtctgcca ccgcgattat cggttgaagg gccgccgaat tgaaattcgc
      781 atgttcgccg atcggatgga agtgatcagc ccgggcgggc tgccgggttt tatcacggtc
      841 gataatttag tcgaagaaca tttttcgcgc aatccgcggt tggtggccgg cctgtttcat
      901 tggggctata tcgaagagct ggggctggga atcgatcgca tgatcgaaga aatgaccctc
      961 ggtggtcatg cgccgccgca cttcaaagcg acaccgtatt cgtttaccgt tactttgtcg
     1021 aacatcaagg aacgcaaagc gataccggtg tgggaaaagg cgatgaatga acggcaggcg
     1081 catgcgttga cgtatctgcg cgagcacggt cagatcacca atcgggagta ccagcagatt
     1141 tgtccggatg tatcggccga aacgctgcgc ttggatttat cggacttggt cgatcgcggc
     1201 gcgttgatgc gcatcggcgc gaaaaaaggc acgtattata ttttgaagta aaggctttta
     1261 acgtgacatc ggttttgttt ggcttgcaat ctccggttct cctcgcgctc agcgcctgcg
     1321 ctggctacgt caggttggcg gtcttcgtcg tgcggcatgg tcaatcggtg catcatcggg
     1381 tatgatcgcc ggcatgcgtt atcgatatcc ggcggtctat cgagtgcagg cattatttgg
     1441 cgcgctgctg gctggcatca tcttgatggg ctccgtcatc gccgaaattg aatctgccaa
     1501 tttgccggat gctttcctgc atgcctttgg tttcatcgtc agtctgatga tcttgatctt
     1561 aggactcgat ttcggcacac gcacaatcga gatcacggcc gccggtttgc agacgcgctg
     1621 gctgcgaaaa tcttttatcg cttggtcgca tattctcgat tggtcataca ggcctctggg
     1681 tttgatccat attagattga atcgaggtct gggcttatac atctggccgt taatggatgg
     1741 ctatacggaa attctcgccg cgatcgatca acatcgagac cagctgacta cgtgatcgcg
     1801 agaatctgag atagtgtgac tagtgttgag caagtggtga caattgcaca ctgatcaact
     1861 gcggtcggtg gtcagccgtc tgcggtcgga atccaaatgc ccaagtccaa ttcaatccat
     1921 agtaaccccg aacaaaaaac attgatggtc aaatggaaag acggatcgat cagcgagatc
     1981 ggctggaaga ttctgcgcga taactgcccg tgcgctgaat gccaggaact gcatggcccg
     2041 gccgatccgt tgaagttgaa actggcgccg aattacaaca tggtcagtgt ggactacgt
//
LOCUS       DASRTJ010000563         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_263031,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000563 DASRTJ010000000
VERSION     DASRTJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263031"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFF70_11435"
     CDS             complement(<1..910)
                     /locus_tag="VFF70_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HZY45351.1"
                     /translation="MSECCDTADVLTLGEVRAQDTSRVLRLHIALAGQPNVGKSTVFN
                     ALTGLNQHVGNWPGKTIDQKIGVHQRGDLTLDVVDLPGTYSLTANSPEELIAREYVLT
                     QRPDVIIAVVDASILERSLYLVAELVQLGLPLVVGLNMLDVARQNGTRIDTAKLSQTL
                     SVPVIELIAAQGKGIDRLIDAAIETARRAVGTIQPLRLKPDLESALSEIETLIAAHLP
                     DEYPLTWSAQKLLEGDPVIEKIVRRSLSAVEGRSLDGLRQAQSSANISIASARYDWIA
                     QAIRSAVTRTPSTSLTWTARIDRITTH"
     gene            complement(910..1140)
                     /locus_tag="VFF70_11440"
     CDS             complement(910..1140)
                     /locus_tag="VFF70_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZY45352.1"
                     /translation="MSMGLDELTSGYSAEIIALEQSGSIAGRLAALGFVPGREVSMVQ
                     NFGHGPLIVAVINTRVALGRREAAGIRVRREM"
     gene            complement(1137..1583)
                     /locus_tag="VFF70_11445"
     CDS             complement(1137..1583)
                     /locus_tag="VFF70_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZY45353.1"
                     /translation="MNDFLSQASRVLAIDGGRMTQPRRIILEVISSSHEHLTADQILD
                     RAKQSDRHINLSTVYRTLAFLKAHQLIEPRYFDQDTHHEVFEPASSTEHYHFTCTQCG
                     AVIEFQSRHVPALRRQLRDEFDAEVTHACVCLTGLCLTCREARTKS"
     gene            complement(1710..2117)
                     /locus_tag="VFF70_11450"
     CDS             complement(1710..2117)
                     /locus_tag="VFF70_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45354.1"
                     /translation="MSASRRRSVGATVIWLGVLVWLPFIVLIMNKQSVSIFPFLALHL
                     SGVLGGWSLRQSADRMEGSVQPASGKGRRRKIVSRVLIYIGVLAWAPYLYLDKIAGVE
                     TAIGPFLAAHLTGVLSGVALRLSVEFDRSAQQP"
     gene            complement(2135..2776)
                     /locus_tag="VFF70_11455"
     CDS             complement(2135..2776)
                     /locus_tag="VFF70_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZY45355.1"
                     /translation="MASLHLHCNAQWVQVCHRLNFIGEGRIMDIFKAIYERQSIGHVN
                     PDRVPRELIEKILATGVQAPNHYKVRPWQFIVLTGSARERLGEIMAQSLHKRNPEASD
                     EALSKERHKPLRAPVLIAVGVHRPGLEKVLDIENVVAVSAAMQNMQLAAHALGLASMI
                     RSGDPVTDPHVKDFLGLAHDQHLLGFLYIGYPIDEHKPVERPAFEDRTTWLEE"
     gene            complement(2929..3774)
                     /locus_tag="VFF70_11460"
     CDS             complement(2929..3774)
                     /locus_tag="VFF70_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZY45356.1"
                     /translation="MLAGFLLALREGIEAALVVGIVLGVLKRMNRMDRARRVWLGVGL
                     AAAISLIVALILNLLNAEFDGPGEQAFEGLTMLAAAGVLTWMIFWMQKQGRSVQTGLE
                     SNVTSALSRGQDWALFSIAFFAVVREGVELALFLTAVNFSSTPAAGVEAPILGWLGGL
                     LGLIAAVIVGWLMFDTTVKLNLRRFFQFTSIVLIVFAAGLVGHAIHEFNELGWIPNII
                     PQWYDFNHVISESTVFGQFLKALFGYSAAPSLTESIGYVGYFVVTFFFARWIDRRPVR
                     SAATA"
     gene            3984..>4229
                     /locus_tag="VFF70_11465"
     CDS             3984..>4229
                     /locus_tag="VFF70_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY45357.1"
                     /translation="MHRQKNDTAGLPLPPPFIVLGFLIAGLILSALFPIDLLPFALRL
                     LLGAALVAIGFFIALRAFQVMMSADTPIDPFEPTTAIV"
BASE COUNT         1038 a         1225 c         1052 g          914 t
ORIGIN      
        1 gatgagtggt gatgcgatcg atccgcgccg tccaggttaa tgaagtcgat ggagtgcgcg
       61 taaccgccga tcggatggcc tgcgcgatcc agtcgtagcg cgcgctggcg atcgagatat
      121 tggcggacga ctgcgcctgc cgcaacccat caagcgatct cccctcaacg gccgatagcg
      181 atcgccgcac aattttttca ataaccggat cgccttccaa caatttttgc gcgctccaag
      241 ttaacgggta ttcatccggc aaatgagccg caatcaacgt ttcaatttcg gacaacgcac
      301 tttcgagatc gggcttcaag cgcagcggct ggattgttcc cacggcgcgg cgagcggtct
      361 cgatcgctgc atcgatcaat cgatcgatgc cctttccctg cgcggcgatc aattcgatca
      421 ccggcacgct caatgtctgc gacaacttgg ccgtatcgat gcgcgtgcca ttctgccgag
      481 ctacatcgag catgttcaag ccgacgacca acggcaggcc caactgcact aactcggcca
      541 ccagatacaa actgcgttcc aagatcgaag catcgaccac ggcgatgatt acatcggggc
      601 gctgcgttaa cacatactcg cgcgcgatca attcttccgg cgagttggct gtcaaactat
      661 aggtgcccgg taaatcaacc acatctaatg tgagatcgcc gcgttgatgc acgccaatct
      721 tttgatcgat cgttttgccc ggccagttgc cgacgtgctg attgagcccg gtcagcgcat
      781 tgaacacggt cgatttgcca acgtttggtt gtccggccaa cgcgatatgc aaacgcagca
      841 cgcggctcgt atcttgcgcg cgaacttccc ctaaagtcaa cacgtcggcg gtgtcgcaac
      901 actcactcat tacatttctc tccgaacacg aatgcccgcg gcttcacgtc ggcccaaagc
      961 cacgcgcgta ttgatcaccg ccacaatcaa cgggccgtgt ccaaagttct gcaccatcga
     1021 aacttcgcgg cccggcacaa agcccagcgc cgccaaccgg ccggcgatcg atccgctttg
     1081 ttccagcgcg atgatctcgg cgctgtaacc tgaagtcaat tcatccagac ccatgctcat
     1141 gatttagttc tcgcttcccg gcatgttagg catagcccgg tcaggcagac acaggcgtgc
     1201 gtgacttcgg catcaaactc gtcgcgcagt tgccgccgca gggcgggcac atgccgcgat
     1261 tgaaattcga tcacggcgcc gcactgcgtg caggtgaaat gataatgttc ggtgctggac
     1321 gcgggttcaa aaacttcgtg gtgcgtatct tgatcgaaat agcgcggctc gatcagctgg
     1381 tgggctttga gaaaggcgag ggtgcgataa accgtggaga ggttgatatg ccgatcggat
     1441 tgcttagctc gatcgagaat ctgatcagcc gttagatgtt cgtgactcga agagatgact
     1501 tccaagataa tgcggcgcgg ctgagtcatc cgtccgccat cgatcgctaa aacgcgcgag
     1561 gcttgcgaga ggaaatcgtt catgaaagta accttattgc aaatgatatg caataaggtt
     1621 actcggttac acccaatcga aatagtgaca gctgtaatct ctcgatcggg cagttgtcac
     1681 aaatccgatc ttctaaatga tcagaataat caaggctgct gggcagatcg atcaaactca
     1741 acgctcagcc gcaaagcgac gccgcttaaa acgcccgtga gatgcgctgc caggaacggc
     1801 ccgatggcgg tttcaactcc ggcaatttta tcgagataca aatacggagc ccaggccaat
     1861 acgccgatgt agatcaaaac ccggctgacg atcttgcgcc gtcggccttt acccgaagca
     1921 ggttgaacac tcccttccat tcgatcggcg ctctggcgca gcgaccagcc gcctaacaca
     1981 ccgctgagat gcagtgccaa aaacggaaaa atcgaaaccg attgtttatt catgatcaac
     2041 acaataaatg gcagccaaac caacaccccc agccagataa cagttgcgcc aacggaccgt
     2101 cgcctgcttg cagacattta catttctcct gtgattattc ttccagccac gtcgttcgat
     2161 cttcaaacgc tggacgttca accggcttgt gttcatcgat cggataaccg atatacagaa
     2221 agccgagcag gtgttgatcg tgcgccagtc ctaaaaaatc tttgacatgc ggatcggtca
     2281 ccggatcacc ggagcgaatc atcgaggcca gacctagagc gtgcgcagcc agctgcatat
     2341 tctgcatcgc cgctgaaaca gcgaccacat tctcaatgtc gagcactttt tccaggccgg
     2401 gccggtgcac acccacggcg atcaacaccg gcgcgcgcag cggcttgtgg cgttctttgc
     2461 tcaacgcttc atcgctcgct tcaggattgc gtttgtgcag cgactgagcc atgatctcgc
     2521 ccagccgttc acgcgcactg cccgttaaca cgataaactg ccacggccga actttataat
     2581 gattgggcgc ttgcacgccc gttgccaaaa ttttttcgat cagttcgcgc ggaacccgat
     2641 cggggttgac gtggccgatc gattgccgct catatatcgc tttgaagata tccattattc
     2701 tcccttcgcc aataaaattg aggcggtggc acacttgcac ccactgcgcg ttgcagtgca
     2761 ggtgtagaga tgccatcgct acctcaatcg aacagtagtt gtggcgtccc gttgccagaa
     2821 cctcaacaag tttccgacat ccacaattgt atcacgcgga tatgtgatga agcccataaa
     2881 acaaaaacct cgccagaata tgacgaggtt tgatcaaagt cgtttggttt aagctgtagc
     2941 cgcactgcgc accggccgtc gatcgatcca gcgcgcgaag aagaacgtca ccacaaaata
     3001 accgacatag ccgatcgatt cggtgagcga gggcgcggca ctgtaaccga ataatgcttt
     3061 gaggaattgc ccaaacacgg ttgattcaga aatgacatga ttgaagtcat accattgcgg
     3121 aatgatattc ggaatccaac ctaactcatt aaattcgtga attgcatgtc cgaccagtcc
     3181 cgccgcgaag acgatcagca cgatgctggt gaattggaag aatcggcgca gatttaactt
     3241 gacggtcgta tcaaacatca accaaccgac aatcacggcg gcgatcaagc cgagcaagcc
     3301 acccaaccaa ccgaggatcg gcgcttccac gccagccgcg ggcgtcgatg agaaattgac
     3361 cgcggtcaag aacaacgcca attcaacgcc ctcgcgcacc acggcaaaga acgcgatcga
     3421 gaataaggcc caatcttggc cgcgcgacaa agcgctcgtc acattcgatt caagtccggt
     3481 ttgaacactg cggccctgct tctgcatcca gaagatcatc caggtcagca cacccgcagc
     3541 agccaacatg gtgagacctt caaacgcttg ctcgcccggc ccatcgaact ccgcgttaag
     3601 cagattcaag atcagcgcga cgatcaaact gatggccgcc gccagaccga cacccaacca
     3661 tacccgccgg gcgcgatcca tgcgattcat tctctttaac acgcccaaca caatgccgac
     3721 aactaatgcg gcctcgatgc cttcgcgtaa agcaagcaag aatcctgcaa gcatagcgct
     3781 aatttctccg tcaggtaatt tgctagagga acatctttca aattgagtct atcggcttat
     3841 cgagagtttg tcaaggataa tggattttga tttttgacgg tcggagcgat tcgctttata
     3901 ctcaactatc gatcttccag atcgaaacgg gctaacaata aaccgagcat cgatctcaat
     3961 gaataacaaa ctttaaggag actatgcacc gacagaagaa cgataccgcc ggattgccgc
     4021 tgccaccacc gtttattgtt ctgggatttc tgatcgccgg attgatcttg agcgccctct
     4081 tcccgatcga tctactaccg ttcgcgttgc gcttactttt gggtgcagcg ctagttgcga
     4141 tcggtttctt catcgccttg cgtgcgtttc aagtgatgat gagcgccgac acgccgatcg
     4201 atccattcga gccaacgact gccattgtc
//
LOCUS       DASRTJ010000564         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_338703,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000564 DASRTJ010000000
VERSION     DASRTJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338703"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFF70_11470"
     CDS             <1..284
                     /locus_tag="VFF70_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45358.1"
                     /translation="AFLTVFITMTAVGAPVTGAIVLALILKGVSKSIPLNAATPDRPI
                     EKMKVDPAGVKELSKGEATFWKIAATLLILAAIGTLTVVMWPTFVQWFS"
     gene            371..973
                     /locus_tag="VFF70_11475"
     CDS             371..973
                     /locus_tag="VFF70_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZY45359.1"
                     /translation="MSDTASTREHAIEQTGSMPGIGNRKLGMWLFLASEILFFTGLIG
                     MFLAYRGRPGGADFLAHLDIPLTSLNTFLLLASSFTVVLSIAAVQSGRQNLLKVWLLA
                     TIVLGATFVSIQAIEYARFGDEGFGLATNAATGAFFVLTGFHGLHVIVGVLWCVGVLL
                     RALRGGFGQNNYLGVELFGLYWHFVDVVWIILFTVIYLLR"
     gene            985..1272
                     /locus_tag="VFF70_11480"
     CDS             985..1272
                     /locus_tag="VFF70_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZY45360.1"
                     /translation="METTKHAKTSTYVAVFVALAIVTGVEIFLGQNLPGAAKLIVLLA
                     LALVKALLVALIFMHLRYDSKIYRWFLIFPIFMALLLIVIVIIQYMTFPIG"
     gene            1435..2361
                     /locus_tag="VFF70_11485"
     CDS             1435..2361
                     /locus_tag="VFF70_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZY45361.1"
                     /translation="MNRKWLGALSGLIAASIWGGMYVVSDAVLDVVPPATLVLMRYIV
                     AVPVLLLAFVIVRDRGVAKRDWGLIALTSLVGFGVSLLAQFAGTKLSTAAAGALITSA
                     TPAFIVVFAWLILREAAGARQWIGLALATSGVLIVSLLGNQSATVEATEPLLGNLLLI
                     VAAVTWALYSVLAKSLTKKYSALAVTLLVTAIGIPLVAPVAAIELRTQTIGLLTPSII
                     AGILYIGIGSTAIAFFLWNKSFELLDAASASLFFFAQPIVGVLLSAIFRGQQLDGSFF
                     IGGGLILLGALLGMLSPHTEPIERRKPIGVEE"
     gene            2450..2950
                     /locus_tag="VFF70_11490"
     CDS             2450..2950
                     /locus_tag="VFF70_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HZY45362.1"
                     /translation="MTFRLTKTKAELLESIQTEWDALQLALDGISEEQMLLRGVVEDW
                     SIKDVLAHITFWQVRLVTSMFKAEKGFKPETTGGGAGVDRLNQQNYLDQKDRDFEAIW
                     DDFESSYQQILKRLENWAEAMLFEPKHFVWMQGDPFARYVEGDSAEHYAEHAEHIRAW
                     RKSKEF"
BASE COUNT          656 a          784 c          888 g          806 t
ORIGIN      
        1 aagcgtttct gactgttttc atcacgatga ctgcagttgg cgcacccgtg acaggtgcaa
       61 ttgtgctggc cttgattctc aaaggcgtca gcaagagcat accgttaaac gccgctacgc
      121 ccgatcgacc gatcgagaaa atgaaagttg atccggcggg cgtgaaagaa ttgtcgaagg
      181 gcgaagcaac cttctggaaa atcgccgcaa cgctgttgat tctggccgcc atcggtacct
      241 tgaccgtagt gatgtggcct acgttcgttc agtggttcag ctaaataaat aatcttgcga
      301 tggtatgtga gtcgacagac gatcgatgca ttcaaccctc gcaaggcttt ctgaggagga
      361 ggcgttctac atgagtgata cagcatcaac ccgcgaacat gccatagaac aaaccggatc
      421 gatgccgggg atcggcaatc gaaaactcgg catgtggctg ttccttgctt cagaaatatt
      481 atttttcacc ggtttgatcg gcatgttcct cgcctatcgc ggtcggccgg gcggcgcaga
      541 ctttttggcg cacctcgata tacctttaac gtcgcttaac actttcttgc tactggctag
      601 cagcttcacc gttgtcctgt ccattgccgc agtacaatcc ggccggcaaa atttattgaa
      661 agtctggctg ctcgcgacga ttgtgctggg cgcgacattc gtcagcattc aggcgatcga
      721 gtatgcgcgg tttggcgatg aaggcttcgg gctggcaacg aatgccgcga ctggcgcgtt
      781 cttcgtgctg accggctttc atgggctgca cgtcatcgtc ggcgtattgt ggtgcgtcgg
      841 cgttttgctg cgcgcgctgc gcggcggctt cggccaaaat aattatctcg gcgttgagct
      901 gttcggcctg tactggcact tcgtcgatgt ggtgtggatc attttgttca cggttatcta
      961 tctgctgcgc tgataggaga aatcatggaa acgactaaac atgccaagac atcgacctac
     1021 gtggcagtct ttgtggcgct ggcgattgtg accggcgttg aaatttttct cggtcagaat
     1081 ctaccgggcg cagccaagct gattgtgctg ctagccttag cgctggtcaa agccttattg
     1141 gtggcgctga ttttcatgca cttgagatac gacagcaaga tctaccgttg gtttctgatc
     1201 ttcccgattt tcatggcgct gttattgatc gtgattgtca ttattcaata catgactttc
     1261 ccgatcggct gattgatcga tcctgatcga agtccgactt tcacgctggc ggggtgagag
     1321 ttggacttcg ttttatttat cacccgattg atgatcctgc gctcgatcga ttgattattt
     1381 cgaccgcgag gacacggtcg agaagcggct gaacaacatg ttacaatcat ccccatgaat
     1441 cggaaatggc tcggcgcgct gagcggcctg atcgccgcat cgatctgggg cggcatgtat
     1501 gtggtcagcg atgcggtgct cgatgtggtg cccccagcca cgctcgtttt gatgcgctac
     1561 atcgtcgcgg tgccggtctt gttgctcgct tttgtcatcg tgcgcgatcg aggcgtcgcc
     1621 aaacgcgatt ggggtcttat cgcgcttacg tcattagttg gctttggtgt ttccttgctc
     1681 gcgcaattcg ctggaacaaa actttcaacg gccgcagcgg gtgcattaat caccagcgcc
     1741 acgcccgcct tcatcgtcgt gtttgcgtgg ctgattctac gggaagcggc cggcgctcga
     1801 caatggatcg gtctggcttt agcgaccagc ggagtgttga tcgtttcgtt gttgggcaac
     1861 caatccgcta ccgtcgaagc cactgagcct ttgttgggca atttgttgct tatcgtagct
     1921 gcagtgactt gggctttgta ttctgtgtta gcaaaatcct tgacgaaaaa atattcagcg
     1981 ttggcggtga cgctattggt tacggcgatc ggcattccgc tcgtcgcacc cgtcgccgcg
     2041 attgagctgc gcacccaaac gatcgggctg ttgacacctt cgatcatcgc cggaattttg
     2101 tatatcggta tcggttcgac ggcgatcgct ttctttttgt ggaataagag tttcgaattg
     2161 ctcgatgcgg ccagtgcctc gctgtttttc tttgcccagc cgatcgtggg tgtgctgctc
     2221 agcgcgatct ttcgcggaca gcaactcgat ggatcgtttt tcatcggcgg cgggctgatc
     2281 ttgctcggcg cacttttggg aatgttatct ccgcatacag aaccgatcga gcgtagaaaa
     2341 ccaatcggag ttgaggaatg agctgcgtaa tgaactttgc gagggtttca gcatggatgg
     2401 acaattcgca agtccaaccc tcgcaaggtt ttagtgagga agaatccata tgacctttcg
     2461 actaaccaaa acaaaagcgg aattgcttga atcgatccag actgaatggg acgcactgca
     2521 attggcgctg gatggcatta gcgaagagca aatgctgctg cgcggcgtcg tcgaggactg
     2581 gtcgatcaaa gatgtgctgg cgcatatcac attctggcaa gtgcggctgg tcacgtcgat
     2641 gttcaaggcc gagaaaggtt tcaagccgga aacaaccggc ggcggagcag gcgtcgatcg
     2701 gttgaatcaa cagaattatc ttgaccaaaa agatcgtgat tttgaggcaa tctgggacga
     2761 tttcgaatcg agttatcagc agattctcaa acgcttggag aactgggcgg aagcgatgct
     2821 gtttgaaccc aagcactttg tctggatgca gggcgatccg tttgcgcgtt acgtcgaagg
     2881 cgattccgcc gagcattacg ccgaacacgc cgaacatatt cgcgcgtggc gcaaatcaaa
     2941 agagttttag cagcgcacgt cattgccaga attccatcat gctgcgcgtg atatatcgtt
     3001 gcagaaactc gcggaggcgg ctcccacgcc gcccagttcg ggcatgggag cccgaaccgc
     3061 taagtatctg agcaggagtg tcgcagacac gacgaagcga tctcctctgg tggtcaatga
     3121 gattgccgcg ccgc
//
LOCUS       DASRTJ010000565         6995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_355831,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000565 DASRTJ010000000
VERSION     DASRTJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355831"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..945
                     /locus_tag="VFF70_11495"
     CDS             73..945
                     /locus_tag="VFF70_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45363.1"
                     /translation="MEKISPHVYVESGYRGCTVGAIITPDGVICIDTPLLPADARHWR
                     GQLALLTDKPIRYVIFTDGHRDRVIGQQWLGGVVVAHEQAYEKIRGFGDAFRQQVVEF
                     LAHHGAPQAADEVSHVLQLALPQITIGDGDVLTLHINKPRVIVRPVGGATPGSVWVEL
                     PDQNITFVGDLVTLNTHPFMSEANTLLWLQRLTELRDKKYFAAKIVPGRGGIYRKQDA
                     QKVTDYLIDMRRRMGKLMTQHRGKIDSADLLPDFLDRFPVPHGENERVQRRIRTGLER
                     VHEELRIENRKNRR"
     gene            1084..2142
                     /locus_tag="VFF70_11500"
     CDS             1084..2142
                     /locus_tag="VFF70_11500"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZY45364.1"
                     /translation="MPFQTETLVEVIRGGRLESEHRGAIAVVNAAGKLIARVGDVNLT
                     TYLRSSAKPYQLLPLVESGAADRFGFTDQELAIMAGSHSGEARHVEVVQSILNKIGLK
                     EEALQCGAHVPYSSESANTLSSRGKAPTPIYNNCSGKHSGMLAAAIDRGLSTHDYLDP
                     NHPIQITIREAIADVSGVPIDQVGVCIDGCSAPNFALPLKAAALAFAKLAESLPPFTG
                     GDQAGGSPRRMALARIAHAMSTYPEMVAGEKRLDTDLMRAVNGRVVSKGGAEGFHGLG
                     IFSTDGKPAVGLAMKIGDGDGKRGGHPAVIEALRQLDVLSEKELEALKEYRGWKLTNH
                     RGTEVGDVRAKFEMLFAR"
     gene            complement(2303..4105)
                     /locus_tag="VFF70_11505"
     CDS             complement(2303..4105)
                     /locus_tag="VFF70_11505"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZY45365.1"
                     /translation="MSAATSPSLPRLKPSSTISWLLIFFIAVATFLIVLLSGVVLFEI
                     IFSIRILPGVSVGGIDLGGQTIDQASATIDARLASKFNSTPIDLADADQVFAPTPIDL
                     GLRLDVRATALAAFQTGRNSADAHFEVMFNGVNLAPQIVFDSKIARAYIDQLASKLNR
                     PAIDAGIQLDGVKVIATAARGGRALKIDQTLNALISNARSLKFMRIDLPFEPLVPQIV
                     DATSVAGQLQSALSSNFTLQLENPAPNEASSWDLTPQQIINLLKVERSADGSQLQFTF
                     DADKLKNGLADLAQQINREPEDARFTFNDDTHQLDIIQTAIIGRQINIDTTLQRMTQA
                     LIDGQHHIIIALTLAQPEFGDNAKAADLGITQLIASESTFYAGSSAERMTNIAAAAAR
                     FHSIIVKPGETFSFDHFLGDVSLDTGYAEALIIANGRTIKGVGGGVCQVSTTAFRAAF
                     TAGFPIIERWPHAYRVGWYERGFGPGLDATVFSPDVDFKFKNDTPYHLLIETYSNNVT
                     GKLTFKFYSTSDGRSVNVSAPVTENVVPHGPDIFEDDPTLAVGTKKQVDYAVDGADVT
                     VKRTVTRNGSIVSEDTVFTRYLPWQAVYKVGTGQ"
     gene            complement(4286..5881)
                     /gene="serA"
                     /locus_tag="VFF70_11510"
     CDS             complement(4286..5881)
                     /gene="serA"
                     /locus_tag="VFF70_11510"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZY45366.1"
                     /translation="MHKILITDDIGPAGLALLDAANDVQYTVLKLPPPEKLIEIIGEY
                     DGVITRSGTALTADVFGAAKKLRVAGRAGVGMDNVDVDAATLRGVLVMNTPEANTLAA
                     TELTMTVMLSLCRNLPGANASVKRGEWTRSKFIGTQLNAKTLGVIGLGRIGSRVATRG
                     QAFGMKVIAYDPYISEEVAERLHVHLVSDLDELLTESDIITVHTPLTDETHGMIGAPE
                     IAKMKNGVRLINCARGGIYDEQSLYAALVSGKVAGAAIDVYTAEPPKSDVLKNLLALE
                     NVVATPHIGANTHEAQRDVAVQIVQQVIDALRGINFRNVINLPFAEGVDYRSLAPYMT
                     LAEKIGSLHMQLIRGRIGRVEVEFRGEEVEQHVKPLTVALLKGMLAPILKEAVNYVNA
                     PHLAEERGITVAQTRHPAAEDYSNVILCRVISTKETRLIGGALFLRTQPRFVLLDEYR
                     IDALPNGPALIISNRDVPGVIGTVGTLLGAHHINIAEWRMGRSGPGGMAVSYINIDAP
                     ASNEVLAQLRGLSTILDVQQVTL"
     gene            complement(5931..>6995)
                     /locus_tag="VFF70_11515"
     CDS             complement(5931..>6995)
                     /locus_tag="VFF70_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZY45367.1"
                     /translation="RLFIPGPTDVHPDTLAAQNRSMIGHRGADFEALFARIQPKLRQV
                     FFTNNRVYISTSSGTGLQEASVRNCVAHKVLCCINGAFSERWYEVAVANGKEVTRLDV
                     EWGHAVKPDQIDAALKNGGFDAITLVHNETSTGVVSPIKDIAAVLHDRYPEVTILIDA
                     VSSLGGDKLDFDGWGLDVLLTSSQKCFAVPPGLAFAAVSDRALAKAKGISNRGLYFDF
                     VDLEKYLLKNQTPATPAISLMWALDAALDRMLAEGLDARFARHAQMADRVRGWAEQQF
                     DLFAEPGYRSHTVTCVKNTRSIDVKALNTFLKSKGMQLSDGYGDLKGHTFRIAHMGEI
                     RMEDVAELLACIDEFLMHNA"
BASE COUNT         1518 a         1910 c         2037 g         1530 t
ORIGIN      
        1 gtgcatagga ataacgtgat ttcacttttc actcgtcact catcaactca aatctcttca
       61 caaagaagca tcatggaaaa aatttctccg cacgtttacg ttgaatccgg ttatcgcggt
      121 tgcaccgtcg gggcgatcat cacgcccgac ggagtaatct gcatcgatac gcctttgctg
      181 ccggccgatg cgcgtcactg gcgcggacaa ttggcgctgt tgaccgacaa gccgatccgc
      241 tacgtgatct tcaccgacgg ccaccgtgat cgggtgatcg gtcagcagtg gctaggtgga
      301 gtcgtggtgg cgcacgagca ggcgtatgaa aagatacgcg gtttcggcga tgccttccgc
      361 cagcaggtcg tcgaattttt ggcgcaccat ggcgcgccgc aagccgccga tgaagtttcg
      421 catgtgctgc aactggcact gccgcaaatc accattggcg acggcgatgt gttgacgctg
      481 cacatcaaca agccgcgcgt gatcgtgcgg cctgtcggcg gcgcgacccc cggcagtgtg
      541 tgggtggagc tgcctgatca aaacattacc ttcgtcggcg atctggttac gctcaatacg
      601 catccgttca tgtcggaagc caatacgctg ctgtggttgc agcgcttgac cgagctgcgc
      661 gacaaaaaat attttgcggc taagatcgtg ccgggacgcg gcggcatcta tcgcaaacag
      721 gatgcgcaga aagtgactga ctacttgatc gatatgcgcc gccgcatggg caaactgatg
      781 acccagcatc gcggcaagat cgattcagcc gatctgctcc cagactttct cgatcgattt
      841 ccggtgcctc acggcgagaa tgagcgtgtg cagcgtcgga ttcgaacggg tcttgaacgc
      901 gtgcacgaag aacttcggat tgagaatcgg aagaatcgca gataaatgac cgcaaactga
      961 tgaccggaga ccgcagctga ttatttactc atgatcaagc gcggtcttcc gtctgcttgc
     1021 cctagtcgcg aagcggagag agtcgtcttc ggtcatttca aattaaatca cagggacttc
     1081 agcatgccat ttcaaaccga aacccttgtc gaagtcatcc gcggcgggcg gctcgagtcc
     1141 gagcatcgcg gcgcgattgc ggtagtgaac gctgccggca aattgatcgc gcgcgttggc
     1201 gatgtgaacc tgaccacgta tttacgatcg agtgccaagc cctatcagtt gctgccgctc
     1261 gtcgaaagcg gtgcggccga tcggtttggc ttcaccgatc aggagttggc gatcatggcc
     1321 ggttcgcatt caggcgaagc ccggcacgtt gaagttgtcc aatcgatctt gaacaagatc
     1381 ggtttgaaag aagaggcgct gcaatgcggc gcgcatgtgc catatagttc cgaatcagcg
     1441 aatacgttgt catcgcgagg caaagccccg acgccgattt acaacaactg ttccggcaaa
     1501 cattccggca tgttagctgc cgcgatcgat cggggcttga gcacgcacga ttatctcgat
     1561 cccaatcacc cgattcagat taccatccgt gaagcaatcg ctgatgtcag cggcgtgccg
     1621 atcgatcaag ttggcgtctg catcgatggc tgctctgcac cgaactttgc tctgccgttg
     1681 aaagcagccg cgttggcctt cgcgaaattg gccgaatccc tccctccgtt tacgggggga
     1741 gatcaagcgg ggggttcgcc gcgccgcatg gcgctggcgc gcatcgcgca cgcgatgtca
     1801 acatatcctg agatggttgc gggcgaaaaa agattggaca ccgatctcat gcgcgcagtg
     1861 aatggccgcg tcgtaagcaa aggtggcgcg gaaggttttc atggcctcgg aattttttcg
     1921 actgatggta aacccgcagt aggtttagcg atgaagattg gcgacggcga tggtaagcgc
     1981 ggtggacatc cggcggtcat cgaagcgctg cggcagttgg atgtgttgag tgagaaagaa
     2041 cttgaagcgt tgaaagagta tcgaggttgg aaattgacca atcaccgagg taccgaagtc
     2101 ggtgatgtgc gtgcgaagtt cgagatgctg tttgcgcgat gaaatctagt gcgtagtgcg
     2161 taaacaccgg gcgaggctca agcgtctttg cgaggagtgc agcgacgaag caatctcacc
     2221 ggattgtcac agagatttct cacttcgttc gaaatgacaa ttaagcataa gccctaaaga
     2281 tcagaaatca tcgatcagag attcattgcc ccgtccccac cttatacacc gcctgccacg
     2341 gcaagtaacg cgtgaaaacc gtatcctctg aaacgatcga gccattccgc gtgactgtgc
     2401 gcttcaccgt aacatccgca ccatcgaccg cgtaatcaac ttgtttcttt gtccccactg
     2461 ctaaagttgg atcgtcctcg aaaatatccg ggccgtgcgg cacaacattc tcggtgacgg
     2521 gcgccgatac attcacgctg cggccatcgc tggtgctgta aaacttgaaa gtcaatttgc
     2581 cggtcacgtt gttggaatag gtttcgatca gcaagtgata cggcgtgtca tttttgaatt
     2641 tgaaatcgac gtcgggactg aagacggtcg catcgaggcc ggggccgaaa ccgcgctcat
     2701 accaacccac gcgatacgca tgcggccaac gctcgatgat cggaaagccg gcggtgaacg
     2761 ccgcgcgaaa cgccgtggtt gaaacttggc acacgccgcc gcccacgccc ttgatcgtcc
     2821 ggccattggc gatgatcaac gcttcggcat aaccggtatc caaacttaca tcacccaaaa
     2881 aatgatcaaa cgaaaaagtt tcgccgggct tgacgatgat gctgtgaaag cgcgcggcgg
     2941 cggcggcgat gttggtcatg cgctcggcgc tggagccagc ataaaacgtg ctctcgctgg
     3001 cgatcagttg cgtgatgcca agatcggctg ccttagcgtt atcgccaaat tccggctgcg
     3061 ccaaagtcag cgcgatgatg atgtggtgtt ggccgtcgat caatgcttgc gtcatccgct
     3121 gcagcgttgt gtcaatattg atctggcggc cgatgatcgc ggtttggatg atatccaatt
     3181 gatgcgtgtc gtcgttgaaa gtgaagcggg cgtcctctgg ctctcgattg atctgctggg
     3241 cgagatcggc cagaccgttc ttgagtttat cggcgtcgaa cgtgaattgc agctgtgagc
     3301 cgtccgccga gcgttccact ttgagcagat tgatgatctg ctgcggtgtg agatcccaac
     3361 tcgacgcttc gttcggcgca ggattctcta attgcaaggt aaaattgctg cttaaagccg
     3421 attgcagctg gcccgcgacc gaagtcgcat cgacgatctg tgggacgagt ggctcaaagg
     3481 ggagatcgat gcgcatgaac ttgagcgagc gggcgttgct gattagagcg ttcaaggtct
     3541 gatcgatctt caacgcccgc ccgccgcgag ctgccgtggc gatcactttg acgccgtcga
     3601 gctgaatgcc ggcgtcaata gctggacggt ttaattttga ggcgagttga tcgatgtagg
     3661 cacgcgcaat ctttgaatcg aagacgatct gcggagcgag attcaccccg ttgaacatca
     3721 cttcaaaatg cgcgtcggca ctgttgcgac cggtctggaa ggcggccaat gctgtggcgc
     3781 ggacatccaa gcgcaggcca agatcgatcg gggtaggggc aaaaacttga tcggcgtcag
     3841 cgagatcgat cggggttgag ttaaactttg aggcgagccg cgcatcgatc gttgcgctgg
     3901 cctgatcgat cgtctggccg ccgagatcga ttcccccaac ggacacgccg ggcaggatgc
     3961 gaatcgaaaa aataatttcg aacagcacca cgccgctgag caagacaatc aggaacgtgg
     4021 cgacggcgat gaaaaaaatc agcagccatg agatggtgga cgaaggtttt agtctgggca
     4081 gactgggcga ggttgcggca ctcatgcgtc aatcatacca cgcgctgatc aatccctgat
     4141 gttacgcagt tgagccgtca tggcgagcag gccatcaggc cgacgaagca atctcagttg
     4201 atagtcattg agattgcttc gccgcaaaac ttgtcctgag cttgtcgaag gcagcgcagc
     4261 tcgcaatgac gttgcgtaac gtattttata acgtcacttg ttgcacatcc aagatcgtcg
     4321 atagcccgcg cagctgggcg agcacttcgt tactggcagg ggcatcgatg ttgatatagg
     4381 acacagccat gccgcccggc ccgctgcggc ccatgcgcca ctcggcgatg ttgatgtggt
     4441 gcgcgcctaa caacgtgcca acagtgccga tgacgccggg cacgtcacgg ttggaaatga
     4501 tcagggccgg accgttaggc agcgcgtcga tgcgatactc gtcgagcagc acgaagcgcg
     4561 gctgggtgcg caggaataac gcgccgccga tcaagcgcgt ctctttagtc gagatcacgc
     4621 ggcacaagat cacattcgaa taatcttcgg cggcggggtg gcgcgtctgc gccacggtga
     4681 tgccgcgctc ttcggccaga tgcggcgcgt tgacgtagtt aactgcttct ttcaagatcg
     4741 gcgcgagcat gcctttgagc aaagccacgg tcaacggctt cacgtgctgc tcgacttctt
     4801 cgccgcgaaa ttccacttcg acgcgaccga tgcggccgcg aatcaattgc atgtgcagcg
     4861 atccaatttt ttcggcgagc gtcatatacg gcgcgagcga tcgataatcg acgccctcgg
     4921 cgaagggcag gttgatcacg ttgcggaagt tgatgccgcg cagcgcatcg atcacttgtt
     4981 gaacgatctg gaccgccacg tcgcgctgcg cttcgtgcgt gttcgcgccg atgtgcggcg
     5041 tcgcgacaac attctccagc gcaagtagat tcttcagcac atccgatttc ggcggttcgg
     5101 cagtgtacac atcgatcgcc gcgccggcca ctttgccgga cacgagcgcc gcatataaag
     5161 attgttcgtc gtagatgccg ccgcgcgcgc agttgatcaa acgcacgcca tttttcatct
     5221 tcgcgatttc cggcgcgccg atcatgccgt gcgtttcatc ggtcaagggg gtgtggacag
     5281 ttatgatgtc agactcggtc aagagttcgt ccagatcgct gacgaggtga acgtgcaggc
     5341 gctcggccac ttcttcggag atatacggat cgtaagcgat tactttcatg ccgaacgcct
     5401 ggccgcgggt ggcaacgcgc gatccgatgc ggcccaggcc gatgacgccc agtgtcttgg
     5461 cgtttagctg cgtgccgata aatttgctgc gcgtccactc gccccgtttg acggaggcat
     5521 tcgcaccggg caagttacgg cacagcgaca gcatgaccgt catggtcaat tcagtcgccg
     5581 ccagcgtgtt tgcttcaggc gtgttcatga ccagcacgcc gcgcaatgtc gctgcgtcga
     5641 catcgacgtt atccatgccc acgcctgcgc gtccggccac gcgcaatttt ttggccgccc
     5701 cgaagacatc cgccgtcagg gcagtgcccg atcgtgtgat caccccgtcg tactcgccga
     5761 tgatctcgat cagtttttcg ggtggcggta gtttcagtac cgtgtactgc acgtcgttcg
     5821 ccgcgtcgag cagcgccagt ccggctgggc cgatgtcgtc ggtaataagt atcttgtgca
     5881 taaatcctcc gagaatgcag cgtgcatcgt tcacaatgca caattatgca ttaagcattg
     5941 tgcattaaga attcgtcaat gcatgccagc aattcagcca catcttccat ccgaatctcg
     6001 cccatgtgcg cgatgcggaa tgtgtggcct ttcaagtcgc cgtagccatc cgatagctgc
     6061 atgccctttg atttcaaaaa cgtattcagc gccttcacat cgatcgatcg ggtgttcttc
     6121 acgcacgtca ccgtgtgcga tcgatagccc ggctcggcga acagatcgaa ttgttgttcg
     6181 gcccatccgc gtactcgatc ggccatttgc gcgtgacggg cgaagcgtgc gtcgagacct
     6241 tcagccaaca ttcgatcgag cgcagcgtca agcgcccaca tcaaggagat cgcgggcgtg
     6301 gcgggcgttt gatttttcaa taaatatttt tccaagtcga cgaaatcgaa atagaggccg
     6361 cgatttgaaa tccctttggc tttagctagc gcccgatcgg atacagcggc aaatgctaag
     6421 ccgggcggca cggcgaaaca tttctgtgat gaagtcaaca acacatcgag tccccaaccg
     6481 tcgaaatcga gcttgtcacc acccagcgat gagactgcat caatcaggat cgttacctca
     6541 ggatatcgat catgaagcac agcggcaatg tccttgatcg ggctgacgac gccggttgag
     6601 gtttcgttgt gaacgagcgt gatcgcatcg aagccgccgt tcttcaaagc ggcatcgatc
     6661 tgatcgggtt tcacggcgtg tccccattca acatcgagcc gtgtgacttc tttgccgttc
     6721 gcgacggcaa cttcgtacca gcgctctgaa aacgcgccgt tgatgcaaca caacactttg
     6781 tgtgccacgc agttgcggac ggaggcttct tgcaggccgg tgccggacga cgttgaaatg
     6841 tagacgcgat tgttagtgaa gaagacttgc cgcagtttgg gttgaatgcg cgcgaacagc
     6901 gcctcgaagt ctgcgccgcg gtggccgatc atcgatcgat tttgtgcggc caaagtatca
     6961 ggatgcacat cggtggggcc ggggataaat aaacg
//
LOCUS       DASRTJ010000566         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_73790,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000566 DASRTJ010000000
VERSION     DASRTJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73790"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFF70_11520"
     CDS             <1..1179
                     /locus_tag="VFF70_11520"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZY45368.1"
                     /translation="FLFLVAGVYGVFMSLDLFLLIFFYELSIFPMYFLIANWGWPVTR
                     EYAAMKLTLYLLIGSIVALIGLLAVYFSADSVAMGLQPGEHTFSMLKLAQGNIPLAVQ
                     RFWFLPIFLGFGVLAGIWPFHNWSPDGHVAAPTAVSMIHAGVLMKLGAYAALVTAVKL
                     LPDAAKEFLPFIIFLVTINVVYGAAIAMVQKDFKYVIGFSSVSHMGLVTMGFATMNQV
                     GLNGAVLQMFSHGVMTALFFSVVGMIYDRTHTRQIPELGGFVKYFPLVTIAFIIGGLV
                     SMGMPGLSGFVAELQIFMGLWSGPANGAIYPWYPIIAIIAIWGVVLTAAYVLRVVQQV
                     FFGDWDPHKWHDLRPINLGDKFVLVTFSLILIVVGLFPSVLMNMISVGTAPVVKLLGG
                     "
     gene            1186..2676
                     /locus_tag="VFF70_11525"
     CDS             1186..2676
                     /locus_tag="VFF70_11525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZY45369.1"
                     /translation="MTAEQLNQGLWAILPEILLFVLAAIVIGVDLIQRGQNRRALGWL
                     SIVGVIGVLVLVVFQTAPMVTSNATYDPFFSGMIRIDLTVQLFRIMFLVAAALTCLIS
                     LDFKPMKQSSEYYALVLFGTLGMGLMAASNNILMLYLSLETTSICLYMLAGFMRDTRP
                     STEAGMKYFLFGAFTSTIMLYGLSLLFGFTGQTTYAGVSAGLSKLVAAQQTLPVMVAL
                     VLVLVGFGFKVAAVPFHMWTPDVYEGAPTPITAFISVASKAAGFAVLIRFFLLVFGSV
                     QDQWVGLIAALAVVTMTLGNVLAISQKNIKRMLAYSSIGQAGYVLIGVAAVGTLGTAS
                     VLFYLAAYVLTNIAAFAVVEVVTNTTGSELIKDMAGFSRKSPAVSFGLLAAMLSLAGV
                     PPLAGFFGKFYVFNAAVGYTDVAGQHSLVWLAIIGVLNSIVALYYYLIILKVVYVDRS
                     EGDTTEVQVSGTLKIGLLIASIGMLVMGIIATPWYDLATQAAKLLH"
     gene            2790..3077
                     /locus_tag="VFF70_11530"
     CDS             2790..3077
                     /locus_tag="VFF70_11530"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZY45370.1"
                     /translation="MNENNPKTSPVASVSEGLKQGSVIGVQIGCMAVVITLGALLLGL
                     WLDGLLHTGPWLTIGLLMVSMPLSVFVVFRFALRAARRLNERHAKPEDKSV"
     gene            3083..>4074
                     /locus_tag="VFF70_11535"
     CDS             3083..>4074
                     /locus_tag="VFF70_11535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZY45371.1"
                     /translation="MKKLFWTAIVIVLIMFVCGCGAIFKLPTLAQYFPATLPPITVEA
                     EPVLCLGGNLLDHCAGGFAITNSLIATLIVDALLIVFFLIAARRPKLIPSGIQNFFET
                     IIDILYNQAQQVAGSNAAKIFPIGATIFLFVFFANYMELVPGVDSIGYVVHAEEKGFE
                     LNASTPAGSPIVFLNPSCPVLTQAEFDKLSADEQQARSGCQPRSSEGAAEGWEVIPAV
                     RTATTDINTTLALALAAVAATQVYGVMGHMPRGQHGVGAVLSGIKRYLSKFFNIGGMR
                     AEGAGKVFGAVDLFASLLEGLSEFVKVISFTFRLFGNIFAGTILIFVVMSLIPYV"
BASE COUNT          762 a         1030 c         1179 g         1103 t
ORIGIN      
        1 ttcttgttct tagtcgccgg tgtttatggc gtgttcatgt cgctcgatct gttcctattg
       61 attttcttct acgagttatc gatcttcccg atgtacttct tgatcgccaa ctggggttgg
      121 cctgtcacgc gcgaatatgc cgcgatgaag ttgacgttgt acttgttgat cggatcgatc
      181 gtcgcgctga tcgggttgct ggccgtctac ttctcggccg attcggtggc gatgggcttg
      241 cagccgggtg agcacacttt cagcatgctt aaactggccc agggcaacat tccactggcc
      301 gtgcagcgat tctggttcct gccgatcttc ttgggcttcg gcgtcttagc aggcatctgg
      361 ccgtttcata actggtcgcc cgatggtcac gtcgccgcgc cgacggccgt ctcgatgatc
      421 cacgcgggtg tgttgatgaa gttgggcgca tatgcggcgc ttgtgacggc agtgaagtta
      481 ctgccggatg cggcgaaaga atttctgcca ttcattattt tcttggtcac gatcaatgtg
      541 gtctatggcg cggcgattgc gatggtgcag aaagacttca aatacgtcat cggtttctcg
      601 tcggtcagcc atatgggttt ggtgacgatg ggttttgcca cgatgaatca agttggcctg
      661 aacggggccg tgctgcaaat gttcagccac ggtgtcatga cggcgctctt cttctctgtc
      721 gtcggcatga tttacgatcg aacccacacg cgacagattc cagaactggg cggctttgtc
      781 aaatactttc cgctggtcac catcgcgttc atcatcggtg gtctggtgag tatgggcatg
      841 ccgggcttga gtggcttcgt cgccgagctg caaatcttca tgggcttgtg gagcggtccg
      901 gccaacggtg cgatctatcc gtggtatccg attattgcga tcatcgcgat ctggggcgtg
      961 gtgctgacgg cagcctacgt gctgcgcgtt gtgcagcaag ttttctttgg cgattgggat
     1021 ccacacaaat ggcatgatct gcgcccgatt aatctcggcg acaaatttgt cttggtcact
     1081 ttctcgctca ttctgattgt cgttggacta tttccgagcg tgctgatgaa tatgattagc
     1141 gtcggcaccg cgccggtcgt caagttgttg ggaggctaac tccgcatgac tgctgagcaa
     1201 ctcaatcaag gcctgtgggc cattctgcct gaaattttat tgttcgtgct agctgcgatt
     1261 gtcattggtg tcgatctgat tcaacgcggc cagaatcgac gcgcgctggg ctggctatcg
     1321 atagtaggcg tgatcggggt attggtgttg gtcgtcttcc agactgcgcc gatggtcacg
     1381 tcgaacgcaa cgtatgatcc cttttttagc ggcatgatcc ggatcgatct caccgtgcag
     1441 ctgttccgca tcatgttcct ggtggcggcc gcgctgacct gcctgatctc gctcgacttc
     1501 aaaccgatga agcaatcgag cgaatactat gccctggttt tattcggcac gctgggcatg
     1561 ggcttgatgg ccgcttcgaa taatatcctg atgctgtatc tgtctttgga aacgaccagt
     1621 atctgtttat acatgttggc cggtttcatg cgcgacacgc gtccctcgac cgaagcgggt
     1681 atgaagtatt tcttgttcgg cgcgttcacc tcgacgatca tgctgtatgg cttgagcttg
     1741 ctgtttggct tcaccggcca aacgacctat gccggagtct cagccgggct gtcaaagttg
     1801 gtcgctgcgc agcaaacact gccggtcatg gtagcgttgg tcttggtgct ggtcggcttc
     1861 ggttttaagg ttgccgccgt gccattccat atgtggacgc ccgatgtgta tgaaggcgcg
     1921 ccgacgccga tcaccgcctt catttcggtc gcatcgaaag ccgcgggctt cgccgtgttg
     1981 attcgattct tcttattagt cttcggatca gttcaagatc aatgggtcgg cttgattgcc
     2041 gcacttgcgg tcgtaacgat gaccctcggc aacgtgctgg cgatctcaca gaagaatatc
     2101 aagcggatgc tggcctattc gtcgatcggg caagcgggct atgtgttgat cggtgtggcg
     2161 gccgtcggaa cactgggcac ggcctcggtc ttgttttact tggcggcata cgtactgacc
     2221 aacatcgcgg ccttcgcggt ggttgaagtt gtgacgaata cgactggcag tgaattgatc
     2281 aaagacatgg cgggcttcag ccgtaagtct ccagccgttt cgtttggact gttggcagcg
     2341 atgctgtcgt tagcgggtgt gccgccgctg gccggtttct ttgggaagtt ctacgtgttt
     2401 aatgcggcag tcggttatac cgatgtggcc ggtcagcatt cattggtctg gctggcgatc
     2461 atcggtgtgt tgaacagcat cgtcgcgtta tactactatc tcatcattct caaagttgtg
     2521 tatgtcgatc gatcggaagg cgatacaacc gaagtgcagg tcagtggtac gttaaagatt
     2581 ggcctgttga tcgcttcgat cggtatgcta gtgatgggca tcatcgccac gccatggtat
     2641 gacctggcaa ctcaagcggc caagttgttg cactaagcgt tgatccttgc ggcggttgtg
     2701 cgatttcgca cattgacgaa ttaaaaatcg tgtgatacca tacgctcaag agcgcgcccc
     2761 ctcaattgag ggcgcgcgca tgttgtaaca tgaacgagaa caaccctaaa acatcgcccg
     2821 tggcatcggt tagtgaaggc ttaaagcagg gcagtgtgat cggcgtgcag attggctgca
     2881 tggcagttgt cattactctc ggtgcgctgc tgttggggtt gtggctggat ggattgctac
     2941 acaccggtcc gtggttgacg attggactgt taatggtcag catgccgctc agtgtctttg
     3001 tagtctttcg atttgcgttg cgcgcggctc gacgattaaa cgagcgccac gctaaacctg
     3061 aggataagtc tgtatgaaat cgatgaagaa actcttttgg acagcaatcg tcatcgtact
     3121 gatcatgttt gtctgcggct gtggagcgat attcaagctg cccaccttgg ctcaatattt
     3181 tccagcgacc ctgccgccca tcaccgtcga agctgagccg gtgttgtgcc tcggcggtaa
     3241 tctgcttgat cattgtgccg gcggctttgc cattacgaat tcattgattg caaccttgat
     3301 cgtcgatgcg ctgctgatcg tcttcttctt gatcgcggcc cggcggccca agctaatccc
     3361 atcgggcatt cagaatttct tcgaaaccat tatcgacatt ctctacaacc aagcccagca
     3421 agtggccgga tcgaatgcgg ccaagatctt cccgatcggc gcgacgattt tcctcttcgt
     3481 gttttttgcc aactacatgg aattagtgcc gggcgtcgat agtatcggct acgttgtcca
     3541 tgccgaagaa aaaggatttg aactaaacgc ctcgacacca gccggatcgc cgattgtatt
     3601 tctcaatccg agctgcccgg ttttgacgca agcagagttc gataaattat ctgcggacga
     3661 acagcaagcc cgatcgggct gccagccccg atcgagcgag ggcgcagctg aaggctggga
     3721 agttattccg gccgtgcgca cggcgacgac cgatatcaat accacgctcg cgttggcatt
     3781 ggcggcggtg gcggccacgc aagtctacgg tgtgatgggt cacatgccgc gcggccagca
     3841 cggcgtgggc gcggtcctgt caggcattaa gcgttacttg agcaagttct tcaacatcgg
     3901 cgggatgcga gcggaaggcg cgggcaaggt tttcggtgca gtcgatctgt ttgccagctt
     3961 gttggaaggc ctgagcgagt tcgttaaagt catctctttc acgttccgac ttttcggcaa
     4021 tattttcgcc ggaacgatct tgatattcgt cgtgatgagc ttgattccat atgt
//
LOCUS       DASRTJ010000567         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_263218,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000567 DASRTJ010000000
VERSION     DASRTJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263218"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFF70_11540"
     CDS             complement(<1..680)
                     /locus_tag="VFF70_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY45372.1"
                     /translation="MAEPLHFETLAIHAGQEPDPSTGAVMTPIYQTSTYVQSGVGNHK
                     GYDYARTANPTRTALENCLAALEGAKYGLCFSSGMAAIDTLLRLVKPGEHVLAADDVY
                     GGTYRLFTKVLSEYGLQFDFIDMSDLAAVRSKLKSNTRLLWLETPTNPLLKINDIAAL
                     AEIAHSRRSNALIKTQVAVDSTFASPYLQQPISLGADFVVHSTTKYIGGHSDVVGGAI
                     LTNDAVAY"
     gene            863..1213
                     /locus_tag="VFF70_11545"
     CDS             863..1213
                     /locus_tag="VFF70_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45373.1"
                     /translation="MSSLMFFADEDEVPLPPREVRITEVKADPLPDGRRVVLTVTLTP
                     FLEYPNLEIVVYRPDGAAERSMSIIGTMDRHLQLTLHIARPEAGGEYRAQIDLLHEGE
                     VIQTEVAKFLIPAA"
     gene            1315..>2009
                     /locus_tag="VFF70_11550"
     CDS             1315..>2009
                     /locus_tag="VFF70_11550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY45374.1"
                     /translation="MGNEALIGKTIGRYQITEHLGRGGMAEVYKAYQPSLDRYVAIKL
                     MHGFLADEKEFLTRFEREAKVVATLRHPNIVQVHDFDVENGVYYMVMEFITGDTLKGL
                     LQELEEKGEWVSTDDAARIILAVGSALKYAHERGMVHRDVKPANVMITEDGQVILTDF
                     GIAKIVTSSNLTASGAMVGTPSYMAPEQGMGQPGDERSDIYSLGVMLYQLMIGRLPYD
                     ADTPLAVVLKHIN"
BASE COUNT          467 a          501 c          568 g          473 t
ORIGIN      
        1 tcgtaagcga cggcatcgtt cgtcaagatc gcgccaccga ccacatcgct gtggccgcca
       61 atatatttcg tcgtcgaatg cacgacaaag tccgcaccga gactgatcgg ctgttgcaag
      121 tagggcgaag cgaacgtgct atcaactgcg acttgggtct tgatcagcgc gttcgatcgg
      181 cgtgagtgtg cgatctcggc cagtgccgcg atgtcattga tcttcagcag aggattcgtc
      241 ggggtttcca gccataacaa tcgggtgttg gatttcaact tcgatcgcac cgcggccaga
      301 tcggacatat cgataaaatc aaattgcaaa ccatactcgc tcaatacttt tgtgaacaag
      361 cggtacgtgc cgccgtatac atcatccgct gcgagaacat gttcgccggg tttaaccaat
      421 cgcaacagcg tatcgatcgc cgccatgccg gatgagaaac acaatccata tttcgcaccc
      481 tctagtgcgg ccaaacaatt ttcgagcgcg gtacgcgtgg gatttgctgt gcgtgcataa
      541 tcgtagcctt tgtgattgcc tacaccggat tgcacgtaag tggaagtctg atagatcggc
      601 gtcatcaccg cgccagtcga tggatcgggt tcttgtccgg cgtgaatggc aagcgtttcg
      661 aagtgcagcg gttcggccat taaaaatcct ttgattgaag tgtggttgag attgtaacag
      721 cgataaacag tcgggtcaaa ctgcgcgcgg cgtggtataa tcgcacgcac cttagatttg
      781 gaaacgaaga ttgaacgcgc tcagtttgcc gatcgcggtt cggcaggtgt ataataattt
      841 tgtgttggtt cgggagtcgg tgatgagcag cctgatgttt tttgcggacg aagatgaagt
      901 gccgctcccg ccgcgcgaag ttcgcataac tgaggtaaaa gccgatccgc tgcctgatgg
      961 tcggcgcgtt gttttgactg taacgttgac gccattcttg gaatatccga atctggagat
     1021 cgtcgtctat cgcccggacg gtgcagcaga acgcagtatg tcgatcatcg gcaccatgga
     1081 tcggcacttg cagttgacgc tgcacattgc gcgacctgaa gccggcggcg agtatcgggc
     1141 gcagatcgat ttgctgcatg aaggcgaagt gattcaaact gaagtcgcaa aatttttgat
     1201 acctgcagcc taaagtgcgt gtactgcccg atcggtttac ccggccgatc ggcttgcaag
     1261 gctaatcact gattgaatcg tttactgtga acatgatagg accccggagg agatatgggt
     1321 aatgaggccc taatcggtaa gaccattggc cgctaccaaa tcaccgagca tttgggtcgc
     1381 ggcggcatgg ctgaagtgta taaagcttat cagcccagcc tcgatcgcta tgtggcgatc
     1441 aaattgatgc acggttttct ggccgatgaa aaagaattcc tgacccgctt tgagcgtgaa
     1501 gccaaagtcg tggctacgct gcgccatccc aacattgtgc aagtgcacga cttcgatgtt
     1561 gagaatggcg tgtattacat ggtcatggaa tttatcaccg gcgacacgct gaagggcctt
     1621 ttgcaggaac tggaagaaaa aggcgaatgg gtcagcacgg acgatgcggc gcgaattatt
     1681 ttagcggtcg gctcggcgtt gaagtacgcg catgagcgcg gcatggtcca tcgcgatgtc
     1741 aaaccggcca acgtcatgat taccgaagat ggtcaagtca ttttgaccga cttcggcatt
     1801 gcgaagattg tgacctcttc caacctcacg gccagcggtg cgatggtggg cacgcccagt
     1861 tacatggcgc ctgaacaagg catgggccag cccggcgacg aacgcagcga catttactca
     1921 ttgggcgtca tgctgtatca actcatgatc ggtcgattgc cttacgacgc cgacactcct
     1981 ctcgccgttg tgttgaagca tatcaacga
//
LOCUS       DASRTJ010000568         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_204333,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000568 DASRTJ010000000
VERSION     DASRTJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204333"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFF70_11555"
     CDS             <1..476
                     /locus_tag="VFF70_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45375.1"
                     /translation="QGQSFQLPDGSTINVKLVTGIMTELRVQRNGQPLPGSPSDPQMR
                     FKTAYGVVFFIAGFNIGLGLLSLATNSSFLRSVGIGTASIGFGVIFLVLGFVIRNQRS
                     KVALILSIVIFGLDFVLGIVLNASNGVNPDTTGIIARIILLMPMAQGISAIDQLA"
     gene            613..1476
                     /locus_tag="VFF70_11560"
     CDS             613..1476
                     /locus_tag="VFF70_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZY45376.1"
                     /translation="MALIAYFKGDPTEYVLLYSGGQLRRSGVALSFTYLVPNTSIVSI
                     PTGTIDSQFILNETSGNFQAVTVQGQLTYRINDPKAIASILNYAIDPKTRGYKSNDPE
                     KVAQRIVNEVQAQVRSELLKMTLEDALKQSADIASNVLAKVKNADVLKSIGVDVLALY
                     ITAIKPTPEMAKALEAEYREGLLVKADQAIYNRRAIAVEQERRIKENELSTAVTLEEK
                     RRELVDLQGQNNLQQADYDAQATAKRFEPFKALDPRMLTALSLKEMAENASKIGNLNI
                     TPDILASLLNK"
     gene            1570..>2382
                     /locus_tag="VFF70_11565"
     CDS             1570..>2382
                     /locus_tag="VFF70_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZY45377.1"
                     /translation="MTTLESILLVTQRTALEDLIARFNTRSQAEFYIEQLAKQNPAYK
                     GGAFTEYQQAHDAYHVSLDLLMKSLPHGVKQQSIERSFLPNFKFSGHDLVITLGRDGL
                     VINTAKYLTNEPILALNPDPTRVDGVLIPFQVTEARGWIERAFQDRALIKLISMAKAE
                     LNDGQVLYGVNDLFIGARSHISARYTIEFGEQSEKHSSSGIIVSTGAGSTGWLQSIVT
                     GAFRIAKGVAQSDLEAPASDQYRLDWSANELTFAVREPFTSKTSQAHIVFGKI"
BASE COUNT          567 a          599 c          677 g          539 t
ORIGIN      
        1 ggcaaggcca atcctttcaa ctgccagacg gatcgacgat caacgtgaag ttggtcacgg
       61 gtataatgac ggaattgcgc gtgcagcgca acgggcagcc gctgccaggc tcgccgtccg
      121 atccgcagat gcgtttcaag acggcctatg gcgtggtgtt tttcatcgcc ggttttaata
      181 ttgggctggg gctgctgtca cttgcaacga atagcagttt cttgcgcagc gtcgggattg
      241 gcacggcctc gatcgggttt ggcgtgatct tcttggtgtt aggtttcgtt attcgcaacc
      301 agcgctcgaa ggtagcctta atcctgtcca ttgtgatttt tggactggat tttgtgctgg
      361 gcatcgtgct gaatgcttcg aacggtgtca atccggacac gacgggcatt attgcgcgca
      421 tcattttgtt gatgccgatg gcgcagggca tcagcgcgat cgatcaattg gcgtgaggca
      481 agctaactgg gttttagacg cggatgagtc ttcatccgcg ttttattttg ccgcttgaca
      541 agaggcaaaa gatagttata attagtgtgt aaattacact aactcaatgc aaccgaacag
      601 gaggaaaaat ctatggctct cattgcttac ttcaagggcg accccaccga atatgtcttg
      661 ctctatagtg gcggccagct gcgccgatcg ggtgtggcgc tgagtttcac ttatttggtg
      721 ccgaacacca gcatcgtgtc gatcccgacc ggcacgatcg attcgcaatt tatcttgaac
      781 gagacctcgg gcaactttca agccgtgacc gttcaggggc aactgaccta tcggatcaac
      841 gatcccaagg cgattgcatc gatcttgaat tacgcgatcg atccaaagac gcgcgggtac
      901 aagtcaaacg atccggagaa ggtggcgcag cgcattgtga atgaagtgca ggcgcaagtg
      961 cgcagtgaat tgctgaagat gacactggaa gatgcgctga agcaatcggc ggacatcgcc
     1021 tcgaacgtct tggccaaggt taaaaatgcg gatgtgttga aatcgatcgg cgtagatgtg
     1081 ctggcgctgt acatcacggc gatcaagccg acgcctgaaa tggcgaaagc gctcgaagcc
     1141 gaatatcgcg aagggctgct ggtcaaagcc gatcaagcga tttacaaccg gcgcgcgatc
     1201 gcggtcgagc aggaacggcg catcaaagag aatgaactga gcaccgcggt cacgcttgaa
     1261 gagaagcgcc gagagttggt ggacttgcaa ggacagaata atttgcagca agccgattat
     1321 gatgcacaag ccaccgccaa acgctttgag cccttcaagg ctttggatcc gcgcatgctg
     1381 accgcgttgt cgcttaaaga gatggcggag aacgcttcga agattggcaa cctgaatatt
     1441 acgccggata ttttggcatc gctcttgaac aagtaaatcc tgaaagaatg cgaggaagcg
     1501 agacagcgag atggcgagaa tttatcccgc tgtctcgaat tctcgccatc tcgcattcat
     1561 tggcagagca tgactacact cgaatccatc ctcctcgtca cacaacgcac ggcgctcgaa
     1621 gatctgatcg cgcgtttcaa cactcgatcg caggcggagt tctacatcga gcagttggcg
     1681 aaacagaatc cggcttacaa gggcggcgcg tttactgagt accagcaggc gcacgatgcg
     1741 tatcacgttt cgctcgatct attgatgaag tcgctgccgc acggtgtaaa gcagcaatcg
     1801 atcgagcgat cgtttttgcc gaacttcaaa ttctccggcc acgatctggt catcacgctt
     1861 ggccgcgatg ggttggtgat caatacagcc aagtatttaa caaacgaacc tatcctcgcc
     1921 ctcaatcccg atccaacgcg cgtcgatggg gtgttgattc cctttcaagt gacagaagcg
     1981 cgtggatgga tcgagcgcgc ttttcaagat cgcgcgctaa tcaaactgat ctcgatggcg
     2041 aaagccgagt tgaatgacgg tcaggtgttg tacggcgtca atgatctgtt catcggcgcg
     2101 cgatcgcaca tttcggcgcg atacacgatc gagtttgggg aacaatcgga gaagcacagt
     2161 tccagcggca tcatcgtatc gaccggcgcg ggatcgaccg gctggctgca atcaatcgtg
     2221 acgggcgcgt ttcggatcgc caagggcgta gcccaatcgg atctggaagc gcccgcttcg
     2281 gatcaatacc ggctggattg gtcagcgaac gaattgactt ttgcggtgcg cgaaccgttc
     2341 accagcaaaa cctcgcaagc gcatattgtg tttggcaaga tc
//
LOCUS       DASRTJ010000569         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_263391,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000569 DASRTJ010000000
VERSION     DASRTJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263391"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFF70_11570"
     CDS             complement(<1..790)
                     /locus_tag="VFF70_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZY45378.1"
                     /translation="MEDDHPVRILFVEDVPADAELAARALRKEGLAFTSTRVETKAAL
                     LKALEEFKPDVIVSDYALPEFDGMQALKLSLERDAAVPFILFTGSLNEETAVACMKAG
                     ASDYVLKDRLRQLPFAVKEVLERKQTRLAQAEAERALRESESRYRELFEAESDAIVLI
                     DNATGNILEANSAAVALYGYDHAELLTKRNVDLSAEPEETQQVTQGTPIIPDQVVTIQ
                     LRFHRKKDGSVFPAEITGRFFIRDGRPVHIAAIRDITERKRAEQE"
     gene            complement(841..1086)
                     /locus_tag="VFF70_11575"
                     /pseudo
     CDS             complement(841..1086)
                     /locus_tag="VFF70_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1425..>2989
                     /locus_tag="VFF70_11580"
     CDS             1425..>2989
                     /locus_tag="VFF70_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZY45379.1"
                     /translation="MPNRLIDETSPYLLQHANNPVEWYAWGTEALSKAKAEDKPIFLS
                     IGYAACHWCHVMAHESFEDEATAKLMNELFVNIKVDREERPDLDSIYMSAVVGMTGSG
                     GWPMSVFLTPDGKPFYGGTYFPPSARYGMPAFREVLKAVSDAWRSKRAEIVENSQGIV
                     GYMQQGDDLVEKAASSPLSIDTLTKAAHNIERNFDRSRGGWGGAPKFPQPMTLEFLIR
                     SYAHSPDEAARSMIDLTLTAIARGGIYDQLGGGFHRYSTDANWLVPHFEKMLYDNSQL
                     ARVYLHAYQTFGTPFYKRITTEILDYLAREMTHPDGGFYSTQDADSEGAEGKFFVWSV
                     AEVRSILGDDALLFTEVYGLTPNGNFQDDHSGLRGNVLSVAHNPIEVAKANGLSLDEL
                     ESKLAQARQKLFDVRERRVKPNRDEKILSGWNGLMLAAFAEAGRDRSLDRSDQYRSIA
                     EKNAAFLLRSMRTPNGRLYRSWKDGQAKLNGYLEDYACVIEGLLAVYQTTFDEQWFIA
                     ARELADTMIKHFT"
BASE COUNT          656 a          828 c          818 g          687 t
ORIGIN      
        1 tttcctgctc cgcgcgcttg cgctcggtga tgtcgcggat cgccgcgatg tggaccggtc
       61 gtccatccct aatgaaaaaa cgccccgtga tctcggccgg gaaaacggag ccatcttttt
      121 tgcgatggaa ccgcaactga atcgtcacaa cctgatccgg aatgattggc gtcccttgcg
      181 tgacctgttg cgtctcctcc ggctctgccg acagatccac attccgtttg gttagcaact
      241 cggcgtggtc gtatccatag agcgccaccg ccgcgctgtt cgcttcgagg atgttgcccg
      301 tcgcgttgtc aatcagaacg atggcatcgg attccgcttc gaacaattcg cgatagcggc
      361 tctcgctttc gcgcaaggcg cgttccgctt ctgcttgggc taaccgggtc tgcttgcgtt
      421 ccagcacttc tttcaccgca aagggcaatt gtctaagccg gtctttgagg acgtaatcgc
      481 tggccccggc tttcatgcag gccacggcgg tttcttcgtt cagcgagccg gtgaatagga
      541 taaagggtac agcggcatca cgttccagtg aaagtttcaa tgcctgcatg ccgtcgaatt
      601 ccggcagcgc gtaatccgaa acgatcacgt cgggcttgaa ttcctctaac gccttcagga
      661 gcgccgcttt cgtttccacc cgcgtcgacg tgaaggccag cccttctttg cggagcgcgc
      721 gcgcggccag ttccgcgtct gcgggaacat cttcgacgaa caggatgcgc acgggatgat
      781 cgtcttccat cggactcctt cattgatgta ggacaaattt ccaactgtgt cctatgtcat
      841 tcagcgcatc ggcggctgat tgagcagcag ccagtacagc cccagatctt tgacggcggc
      901 tatgaacttg tcaaactcca ccggcttgac gatgtaactg ttcacgccca acttgtaact
      961 ctcgacaata tcgctttctt ctttggacga tgtgagcacg acgactggaa tgattttggt
     1021 ccgcggatcg cctttgatcg cccgcaggac ttctagtccg tccactttgg gcagtttcaa
     1081 atcgagaggg ggttgagggc gcaatgccat gatccagctc cacatcattg agcggtaggt
     1141 gtcgacctcg acacagcgaa acgactcgcc tgcgtgcgac tcccccgctg ccgtaccagt
     1201 catggcagac gcgctatggt attctattca caacaataac cgtttgatta tcagtgtatc
     1261 actaattatg tgaatgacaa gattttctga ttgcattgtc gttacagccc ggttacgcca
     1321 cttcgagatc tgctgctgac tcgatggctc gttgacctga catacggctg cacttaagcc
     1381 gtatgttaca atctcctcat taactttcct cggaagaaaa tctcatgccc aatcgattga
     1441 tcgacgaaac cagcccatat ctgttgcaac acgccaacaa ccccgtcgag tggtatgcgt
     1501 ggggcacaga agcgttatcg aaggcgaagg ccgaagacaa accgatcttc ttgagcatcg
     1561 gttatgcggc ctgccactgg tgtcacgtca tggcgcacga atcattcgaa gatgaagcga
     1621 cggccaagtt gatgaatgag ttgttcgtca acatcaaagt cgatcgtgaa gagcggcctg
     1681 atctcgattc gatctacatg agcgcggtcg tcggcatgac gggcagcggc ggctggccga
     1741 tgagcgtctt cctcacaccg gacggcaaac cgttttacgg cggcacttat tttccaccaa
     1801 gcgcgcgcta cggcatgccg gcctttcgtg aagtgttgaa ggccgttagc gatgcatggc
     1861 gatcgaaacg cgctgagatc gtcgagaaca gccaaggcat tgtcggttac atgcagcaag
     1921 gcgatgacct ggtcgagaaa gcggcgagca gcccattatc aatcgatacg ttaacaaaag
     1981 ccgcacacaa catcgaacgc aacttcgatc gatcgcgcgg cgggtggggc ggtgcgccaa
     2041 aatttccgca gccgatgacg ctcgagtttt tgatccgcag ttatgcgcac tcgcccgatg
     2101 aagccgcacg ctcgatgatc gatctcacgc tcaccgcgat agcgcgcggc ggcatatacg
     2161 atcaactcgg cggcgggttt catcgctatt cgaccgacgc gaattggctc gtgccacatt
     2221 tcgagaagat gctgtacgac aacagccaac tcgcgcgagt gtatctgcat gcttatcaaa
     2281 ctttcggcac gccgttttac aaacgcatca cgactgaaat actcgattac cttgcgcgtg
     2341 agatgacgca ccctgatggc ggcttctatt cgacgcagga tgctgacagt gaaggcgccg
     2401 aaggaaaatt ctttgtgtgg agcgtcgctg aagtccgatc gatcttgggc gacgatgcgc
     2461 tgctcttcac tgaagtctat ggccttacac cgaacggaaa ttttcaagac gatcattccg
     2521 gcctgcgcgg caatgtgctc agcgtcgcgc acaatccgat tgaagtggct aaagcgaacg
     2581 gcctgtcgct ggatgaactc gaatcgaaat tagctcaagc ccgccagaag ttgttcgatg
     2641 ttcgtgagcg gcgcgtgaag cccaatcgag acgagaagat tctatcgggc tggaatggat
     2701 tgatgctggc cgcgttcgcc gaagctgggc gcgatcgatc gctggatcga tcagatcaat
     2761 atcgatcgat cgcggaaaag aatgcggcct tcctgctccg atcgatgcgc acgcccaacg
     2821 gacggttata tcgatcgtgg aaagatgggc aggcgaagtt gaatggttac ttggaagatt
     2881 acgcgtgtgt gatcgaaggg ctgctcgctg tgtatcaaac gaccttcgat gagcaatggt
     2941 tcattgccgc gcgcgagttg gccgacacga tgatcaaaca cttcaccga
//
LOCUS       DASRTJ010000570         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_338962,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000570 DASRTJ010000000
VERSION     DASRTJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338962"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1628
                     /locus_tag="VFF70_11585"
     CDS             123..1628
                     /locus_tag="VFF70_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45380.1"
                     /translation="MLKHRRPIIFLIIAFINVALYVGVSTSRGHIGYPLDDAWIHQTY
                     ARNWAETGQLAYVIGQPSAGSTSPLWTLLISIGYRLHIDPYLWTLLLGALCLGLSAWL
                     LARIADRLLPNRKLIAWLVGLACLFEWHMIWAAASGMETMLFIALALIVIDRLLADAR
                     AWSIGFFGGLLIVTRPEGLLLIGLAALLLVTRSANGGPKEFLLTIITALVVVMPGALI
                     NFQASGTFFPNTFYAKQQEYAELMSSASIWLTSIGNMIGAPFVGSQVLLLPGVFYWLI
                     NGGEVEGPPHHKRRLLFRDKSQWVQWLPLIWIGAHTAVYALRLPVAYQHGRYLMPIIP
                     IVLLYGAVGLSIALDRRSGSATPGRLSKLLGPALIGSIVIGLIAFVPIGAAAYAADVS
                     IIEDEMVTVARWLAVTAPPEALIAAHDIGAIGYFARRPLIDLAGLISPEVIPFIRDEG
                     RLQQLIEDRRASYLVTFPDWYPHLAQAPSFAKVFQGGSSLSPQHLTVYAVR"
     gene            1711..2451
                     /locus_tag="VFF70_11590"
     CDS             1711..2451
                     /locus_tag="VFF70_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45381.1"
                     /translation="MPKISVLIVDDHQIFRQGLRDAMSLDAEIQVVGEASTGEEALQI
                     ARELSPRVVIMDVNLPGGMNGLQVVQRMRKEVPGVRTLVLTAYDDEEQLFHAIRGGAQ
                     AYFSKDASPDRIIEGVREISQGRYVIANKVMDEEHVAPWLLHASERFGQAGGELGDET
                     LVPLSPREMEILQHITRGMANKTIAHKLGISHQTVKNHMTSILRKLDVEDRTQAAIYA
                     IRHGWVRLQDTQVQSDPPARQDRSSKLG"
     gene            2444..>3460
                     /locus_tag="VFF70_11595"
     CDS             2444..>3460
                     /locus_tag="VFF70_11595"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZY45382.1"
                     /translation="MANPKRSDIQALTLTQQLTEGTRTELEQAQKELKEIDLLILQTG
                     SEVDRLEQRNAQAVNQLKQLEAALDTVPRADLQVAYTAAQDAQKRMLLMRGQLEKLQS
                     DQHNFGRIVNSLRQTSEALKKIDGGSIGATPAADGDANIQAPIVRIIEAQEQERQRLV
                     RQLHDGPAQSLTNLILQAEICERVFDTDAKRARTELGELKAAVTTTFQKVRDYMFDLR
                     PMMLDDLGLIPTLRRYVDNFNEKSGVPAVLTITGSERRFAAYKEVVIFRVVQELLGNV
                     RQHAQATRVTVLLDATEDTAHVIVEDNGSGFNVNEVLNTAQPKGIGLSTMRERMTMLG
                     GQVQI"
BASE COUNT          767 a          894 c          990 g          809 t
ORIGIN      
        1 gtgctcgatc cgaatcatgt ccacgcgctg gatgcgttgt atgccggctc cgaaataaat
       61 ccgcggcttg aacatgtcaa cgattttgca tcgatacaag tttatcggat tgtgcactac
      121 tgatgctgaa acatcgacgg cccattatct tcttgatcat tgccttcatc aacgtcgccc
      181 tgtacgtggg cgtgtcaacg tctcgtggtc acattggcta tccattagat gacgcatgga
      241 ttcatcagac ttacgcgcgc aactgggccg agaccggaca actcgcctac gtcatcggcc
      301 aacccagcgc cggttcgact tcaccactat ggacattgct gatcagtatc ggctatcgtt
      361 tacacattga tccatactta tggacactgt tgcttggcgc gctgtgtctc ggactaagcg
      421 cctggctgtt agcacgcatc gctgatcgat tgctgcccaa tcgaaaatta attgcgtggc
      481 tcgtcggttt agcgtgtcta tttgaatggc atatgatctg ggcagcggcg tccggcatgg
      541 aaacgatgct attcatagcc ttggcgctca ttgtcatcga tcgattgttg gcagatgcgc
      601 gggcttggtc gatcggattc ttcggcggat tattgatcgt gacgaggcca gaaggtttac
      661 tgttaatcgg tttagctgcg ctgctgctgg taacccgatc agctaacggc ggcccgaaag
      721 aatttttgct cacgataatc actgcgctcg tggtggttat gccaggcgcg ctgatcaact
      781 ttcaagccag cggaactttt tttccgaata cgttctatgc caagcaacaa gaatacgctg
      841 aactcatgag cagcgcatcg atctggctga ccagcattgg aaatatgatc ggcgctccct
      901 ttgtaggtag tcaagtgcta ttgctgcccg gtgtatttta ttggctcatt aatggtgggg
      961 aggtcgaagg acctccccac cataaacgcc gactgctgtt tcgagacaag tcacagtggg
     1021 tgcagtggct gccgctcatt tggatcggcg cacacaccgc cgtgtatgcg ctgcgcttgc
     1081 cggtggctta tcaacatggc cgctatctca tgccgatcat tccgatcgtg ctgctgtatg
     1141 gggcggtagg tctctcgatc gcgcttgatc gccgatcggg ttcagctacg cccggtcgat
     1201 tgagcaagtt gctcggcccg gcgctgatcg gatcgatcgt gatcggcttg atcgcttttg
     1261 tgccgatcgg tgcggcggct tatgcggctg atgtgtcgat catcgaggat gaaatggtga
     1321 cggtcgcgcg ttggctggcc gtcaccgccc cgccggaggc gttaatcgcg gcgcatgata
     1381 ttggggcgat tgggtacttt gcgcgccgcc ccttgataga tctggccggg ctgatctcgc
     1441 cggaagtgat tccatttatc cgggatgaag gccgcttgca gcagctcatc gaagatcgac
     1501 gggcgagtta tctggtgact tttcctgatt ggtacccgca cttagcgcag gctccttcat
     1561 ttgccaaagt gtttcagggc ggctcaagct tgagtccgca gcatttgacg gtctatgcag
     1621 tgcgttagcc aatcgctttg gcttgcatgt gctatccatt tttggtatac tgcccacacc
     1681 agcgtcaccg gtttagatcg aggatttcgc gtgccaaaga tttcagtttt gattgtcgac
     1741 gatcatcaga tctttcgcca gggtttgcgc gatgcgatgt cgctggatgc cgagattcaa
     1801 gtcgtcggcg aagcctcgac cggcgaagag gcactgcaaa tagcgcgtga gctgtcgccg
     1861 cgcgtggtga ttatggacgt caatttgccg ggcggcatga atggcttgca agtggtgcag
     1921 cgcatgcgga aagaagtgcc gggtgtgcgc acgctggtcc taactgctta cgatgatgaa
     1981 gagcaattat tccacgcgat tcgcggcgga gcgcaagctt acttctccaa agatgccagt
     2041 ccggatcgaa tcatcgaagg ggtgcgcgaa atcagtcagg gccggtatgt tatcgctaac
     2101 aaagtgatgg atgaagaaca cgtggcgccc tggttattgc atgccagcga gcgcttcggt
     2161 caggccggcg gcgaattggg cgatgagacg ctggtgccct tatcgccgcg tgagatggaa
     2221 atcttgcagc atattacgcg cggcatggcc aacaaaacga tcgcgcataa gctgggtatc
     2281 agtcatcaaa cggtgaagaa ccatatgacc tcgattttac gcaagctcga tgtggaagat
     2341 cggacgcaag ctgccattta tgctattcga catggttggg tgcgtttgca agacacgcag
     2401 gttcaatctg atccgccggc gagacaagac aggagcagca agcttggcta acccaaagcg
     2461 atccgacatt caagccctaa cgctgactca gcagctgacc gaaggcactc gcactgagct
     2521 agaacaggct caaaaagagc tcaaagagat cgatttgctt atcctgcaga caggttctga
     2581 agtcgatcgc ttggagcagc gtaatgcaca agcggtcaat cagctgaagc agctggaagc
     2641 tgcactcgat acggtgccgc gcgccgatct ccaagtagct tataccgcgg cgcaagatgc
     2701 tcaaaaacgc atgctgctca tgcgcggcca actggaaaaa ctacaaagtg atcagcacaa
     2761 tttcggtcgc attgtcaatt ctctgcgaca gacatctgaa gcgctgaaaa agatcgacgg
     2821 tggatcgatc ggtgctactc cggcagcgga cggcgatgcg aacattcaag cgccgattgt
     2881 gcgcatcatc gaagcgcagg agcaggaacg gcagcgctta gtccgtcaat tgcatgatgg
     2941 cccggcgcag tcgctcacca atctcatttt acaggcagag atttgcgagc gtgtctttga
     3001 tacggacgcc aagcgcgctc gaactgaatt gggcgagctc aaggcggcag tcaccaccac
     3061 ctttcagaaa gtacgtgatt acatgttcga tctacgcccg atgatgctgg atgatctggg
     3121 gctgattcca accctgcgcc gctatgtcga taactttaac gagaagagcg gcgtgccggc
     3181 ggtgctgacg atcaccggga gcgaacgacg ttttgcagct tacaaagaag tcgtcatctt
     3241 ccgggtggtt caagaattat taggcaatgt gcggcaacac gcgcaggcca ctcgagtgac
     3301 ggtgctgctc gacgccaccg aagacacagc gcacgtcatc gtcgaggaca atggcagcgg
     3361 cttcaacgtc aacgaagtat tgaataccgc tcaaccaaaa ggcattggcc tgagcacgat
     3421 gcgtgagcgg atgacaatgc tgggcggtca agtacagatc
//
LOCUS       DASRTJ010000571         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_35861,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000571 DASRTJ010000000
VERSION     DASRTJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35861"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFF70_11600"
     CDS             <1..580
                     /locus_tag="VFF70_11600"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY45383.1"
                     /translation="FGIVHSLLAAYQAKLIAQRVLGHSLATAIYRLAFNLIGVLSIAP
                     ALYWTFALPDRELYRFPEPVNIVALITQAVAGLGIAYSIYQLDFFFFAGLRQLIGANR
                     FALESTSTAHLVTNGLHRYVRHPLYTTSLIFLYLTSPMTINRLALITSFTLYFYIGSI
                     FEERKLVREFGAAYRQYQTQVPRLIPRLHQHL"
     gene            complement(563..1477)
                     /locus_tag="VFF70_11605"
     CDS             complement(563..1477)
                     /locus_tag="VFF70_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZY45384.1"
                     /translation="MHPSTFIIVNPASGSGKTGMRWSGWEAHFRTEGSVFDVGFTAEP
                     GHASRLAREAALKGYTKIISVGGDGTFNEVLNGLIENDRAVADVQLGVLPVGTGSDFA
                     RSLHLPKDPRKAWQHLMSGQVSPLDVGHIDTRRGDTTITHYFANVAGLGFDGEVSDRV
                     NRSGRKSGGTIPYLSTMLASLAKYKNKSVTVTVDDRTIEGRMNSVVVCNAKYFGGGMF
                     IGPHADWSDGQFDVVILGDFSKLEVLANVPRLYKGTHLLHPKVTFLRGRSIRVETKDL
                     MYLQAEGELIGEAPTTFTMLPRLLKVLM"
     gene            1638..2309
                     /locus_tag="VFF70_11610"
     CDS             1638..2309
                     /locus_tag="VFF70_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZY45385.1"
                     /translation="MPRLSDHPSITVDVVMMTLQKNQLQALLIKRDAAPYRGRWAIPG
                     GFVQIAESLENAARRELKEETGVKDVYLEQLYTFGEPKRDPRGRVISVAYIALVSAPL
                     TVRAGTDAREARWWPVTELPPLAFDHDQILEYALTRLRYKIEYSSVGFRLLPETFTLS
                     ELQRAYEIILNEPLDKRNFRRRISEADVIEPLDEIRSGDGRPARLYRFRADAVAEVKA
                     RRLFP"
     gene            2397..>3714
                     /locus_tag="VFF70_11615"
     CDS             2397..>3714
                     /locus_tag="VFF70_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45386.1"
                     /translation="MLPKPLKWPLIAIGGGAMAIVISLGWIGQSAAQGSIGLSANPID
                     PAALNDQNQTGNLLINPDFESGSTPYPGHNNIQIPTGWNIKWYTDIDAATGYSFFQPE
                     VKLDDNTIWPYCDGCAPNVPPRIHTGRFAVDSFKSYASQDTTLYQQVIHIPIGASVTA
                     SAWLHAWVTRCNAFPKNFPPQPAISLYGPNSSLDGSCEPDGTLPESSNRMMVGIDPTG
                     GIDPRAASVVWNLGDPNTPMWNGPYDYYSSTLPVTIFLRGITTSDAKFSDFYFDTASL
                     VYSFPISVTTRQALPWPLTSTVAITLQAPISLTQLNASLADPNGQSIPLTYLGTTGFT
                     PPFISRWEFSPAIAGVHLFTVTAAQLPNPLVQPIDVSVMQYTTIQDHLPPPSPITFTL
                     YSPISLTDFTSVLTDPLGSLTTFNSIGSNYISPTYAFQWTFTSTITG"
BASE COUNT          819 a         1027 c          935 g          933 t
ORIGIN      
        1 atttggaata gttcacagcc tgttggccgc ctatcaagcc aagttgattg cgcagcgcgt
       61 actgggccat agcttggcaa ccgcgattta tcgtctcgcc ttcaatctca tcggcgtgct
      121 atcgatcgca ccggcgctgt attggacgtt tgcgttgccc gatcgagagt tgtatcgctt
      181 tccagagcca gtgaacatcg tcgcgttgat cacgcaagcg gtagcgggtt taggcatagc
      241 gtattctatt tatcaattag actttttctt tttcgccggg ctgcgccagc taatcggagc
      301 taatcgattc gcgcttgaat caacatcgac cgcacacctg gtgacgaacg gcttacatcg
      361 ctacgtgcgg catccgctgt acaccacttc attgatcttt ctgtatctta cttcgcccat
      421 gacgatcaat cgtctcgcct tgatcaccag cttcacactc tacttctaca tcggatcgat
      481 cttcgaagag cgcaagctgg tgcgcgagtt cggggctgcc tatcggcaat atcaaacaca
      541 agttccacgt ttgatcccgc gtttacatca acacctttag caaacggggc agcatcgtaa
      601 acgtcgtcgg cgcttcgccg attaactctc cctcggcttg caagtacatc aaatcttttg
      661 tctccacacg gatcgatcgg ccgcgcagga aagtcacttt gggatgcagt agatgtgtcc
      721 ctttatacaa acgcggcacg ttcgctaaca cttccaattt gctgaagtcg cccagaatca
      781 ctacatcgaa ttggccgtcg ctccaatctg cgtgcgggcc gatgaacatg ccgccgccga
      841 aatatttggc gttgcacacc accactgaat tcatccggcc ttcgatcgtt cgatcatcga
      901 ctgtgacagt tacggatttg ttcttatact ttgccaatga agccaacatt gtcgatagat
      961 atggaatcgt tccgccgctt ttgcggcctg atcgattgac gcgatcagaa acttcgccat
     1021 cgaagccgag gccggcgaca ttcgcaaaat aatgcgtgat tgttgtatcg ccgcgacgtg
     1081 tatcgatgtg acccacatcc agcggcgaaa cttggccgct catgagatgc tgccaagcct
     1141 tgcgtggatc tttgggcaag tggagcgatc gggcgaagtc cgatcctgtg ccgacgggca
     1201 gtacgcccaa ttgcacatcg gccacggctc gatcgttttc gatcaagcca ttcagcactt
     1261 cattgaaggt gccatcgccg ccgactgaaa tgatcttcgt gtatcctttg agtgcagcct
     1321 cgcgcgcgag acgtgaagcg tgacccggtt cagcagtgaa gcccacatcg aacacgctgc
     1381 cttcggtgcg gaagtgcgct tcccaaccgg accagcgcat gccggtcttg cccgaaccgg
     1441 atgccggatt gacgatgata aaagtggatg ggtgcatgtc gcctccctgg gtgagattaa
     1501 tattatagag gcggaagacg aaaattggaa atcggaaatc ggccgtgagt caaactgctc
     1561 tcttcaacag aaagcttgac aagctgagcg gtcggcagta cattggtgta tagacaacac
     1621 caacgagagg ctcacccatg ccgcgcttgt ctgatcatcc ctctattacg gtcgatgtcg
     1681 tcatgatgac cttgcaaaaa aatcaattgc aagcgttgct gatcaaacgc gatgccgcgc
     1741 cttatcgcgg ccgttgggcg atccccggcg gttttgtgca aattgctgaa tcacttgaaa
     1801 atgcagcccg gcgtgaatta aaagaagaga ccggcgttaa agatgtttat ctggagcagt
     1861 tgtatacctt tggcgaaccc aaacgcgatc cgcgcggccg ggtgatcagt gtagcctaca
     1921 tcgcgttggt gtctgcgccg ttgaccgtgc gcgccggtac ggatgcgcgt gaagcgcgct
     1981 ggtggcctgt cactgaattg ccgccgctgg ccttcgatca cgatcaaatt ttggaatatg
     2041 cgctcacgcg tttacgctac aagatcgaat actcctccgt cggttttcgg ttgttgccgg
     2101 aaacatttac gctgagcgaa ttgcaacgcg cctatgagat cattcttaat gagccgttgg
     2161 acaagcgcaa tttccgacgt cggatcagtg aagccgacgt gattgagccg ctcgacgaaa
     2221 tccgatcggg tgatggccgc ccggcgcgct tgtatcgatt tagagcggat gcggtcgccg
     2281 aagtaaaagc gcgccgattg tttccgtaat ttcagtatca ttgtcttatt tcctcttggc
     2341 tattttcctc gtagaattat tctatcttcg gaagttagga caaaggatcg tcattcatgc
     2401 tgccaaaacc attgaagtgg ccgttgattg cgattggggg tggggcgatg gcgatcgtga
     2461 tctcacttgg ctggatcggt cagtcagctg cgcagggatc aattggtctg tccgctaatc
     2521 cgatcgatcc ggcggcgctc aacgatcaaa atcaaaccgg aaacttattg atcaatccag
     2581 attttgaaag tggatcaacg ccttatccgg ggcacaacaa cattcaaatc ccgaccggct
     2641 ggaacatcaa atggtatacg gatattgatg ctgcgaccgg ttattcgttt tttcaacccg
     2701 aagtcaaatt ggacgataac accatctggc cgtattgcga tggttgcgcg ccgaatgtgc
     2761 cgccgcgcat tcataccggc cgatttgcag tcgattcatt caaatcgtat gccagtcaag
     2821 atacaacttt gtatcagcaa gtcattcaca ttcctatcgg cgcaagcgta accgccagcg
     2881 cttggttgca tgcttgggtc acacgctgta atgcgttccc gaaaaacttt ccgccgcaac
     2941 ctgccatatc cttgtacgga ccgaatagct cgctcgatgg ttcgtgtgaa cccgatggca
     3001 cgttgccaga gtcatcgaac agaatgatgg tcgggatcga tccgaccggc ggcatcgatc
     3061 cgcgcgccgc gagcgtggtc tggaaccttg gcgatcccaa tactcccatg tggaatggtc
     3121 cgtacgatta ttattcatcg actttgccgg tcacgatctt tttgcgtggc attactacca
     3181 gtgacgcgaa gttcagcgac ttttacttcg atacagccag tctggtctat agctttccga
     3241 tcagcgttac cacgcgacag gctcttccct ggccactgac ttcaaccgtc gccatcacgc
     3301 tgcaagcgcc tatttctttg acgcagctca acgcgagcct cgctgatccg aacggccagt
     3361 caatcccttt gacgtatctc ggtacaacgg gctttacacc tccctttatt tctcgctggg
     3421 aatttagtcc ggcgattgcc ggggtgcatc tattcacggt gaccgccgcc caattaccca
     3481 atccgttagt ccagccgatc gatgtgtcgg tgatgcagta caccactatt caagaccact
     3541 tgccgccgcc gtcgccgatc accttcacgc tctattcgcc gatttcgctc acggatttca
     3601 cttcggtgct gactgatccg ctcggctcat taacgacctt caactctatc ggatcgaatt
     3661 acatcagccc gacgtatgca ttccaatgga cgtttacctc gacgatcact ggtg
//
LOCUS       DASRTJ010000572         5253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_74027,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000572 DASRTJ010000000
VERSION     DASRTJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74027"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1943)
                     /gene="dnaK"
                     /locus_tag="VFF70_11620"
     CDS             complement(21..1943)
                     /gene="dnaK"
                     /locus_tag="VFF70_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZY45387.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPVVIPSAEGSRLIPSVVAVNP
                     KTHERSVGQVARRQAITNAENTVYSIKRLMGRKFKDDQVQKALKRLPYKVTEAPNGDC
                     RVTMDDKDYSPPEVSAMILQKMKQDAEAYLGETVTQAVITVPAYFNDSQRQATKDAGK
                     IAGLEVLRIINEPTASSLAYGLDQKKDEVIAVYDLGGGTFDISILDVGDGVFEVKSTN
                     GDTFLGGDDFDQRIIDYIADEFKREQGIDLRGDRQALQRLKESSEKAKIELSSLMETE
                     INLPFITADASGPKHLQLKLTRSKLEQLTADLIERSIAPCKRALEDASMSPDKIAEVV
                     LVGGMTRMPAVQEAVRKFFQREPHKGVNPDEVVAIGAAIQAGVLGGEVKDVLLLDVTP
                     LTLSIETLGGVATALIQRNTTIPTKKSQVFSTAADGQTSVEIHAMQGERPMATDNKSL
                     GKFILDGIPPAPRGTPQVEVTFDLDANGILNVSASDKATGRTQHITITASSGLSDDEV
                     NRMVKDAEKFAGEDAKRKERIEVRNSADNMVYTAEKSLRDFGEKIPADSKTDIESKAT
                     ALKSVLESASVEELKHKTEELSQAIQKIGSIMYEQPGGATPPPTGDEGSSSGPTAPTG
                     SAGPDGDVIDGEFSKN"
     gene            complement(2020..2559)
                     /locus_tag="VFF70_11625"
     CDS             complement(2020..2559)
                     /locus_tag="VFF70_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZY45388.1"
                     /translation="MSDDVIKDEPIEPAPDDAAEQPIDLFKDLNEQIKQAQAQAAENL
                     EGWQRAKADFINYKRRVELERTEMFNSISGDILLRVLPAVDDFDRAVQTLPEDLQDNS
                     WVNGVLLIQRKLSGLLDQSGVKIIPVQPGDEFDTAIHEAVTHEESEQVASGHVIAEVQ
                     HGYTIGERVLRPALVRVAK"
     gene            complement(2691..3746)
                     /gene="hrcA"
                     /locus_tag="VFF70_11630"
     CDS             complement(2691..3746)
                     /gene="hrcA"
                     /locus_tag="VFF70_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZY45389.1"
                     /translation="MDELTSRQQLILGLVVREYVSSETASEDGVHRVQPVGSKVLVER
                     YNLDYSPATIRSELAYLEQHGYLMHPHTSAGRIPTDSGYRYFVEVLMDDDELPANEQR
                     TIDHQFHQARLDLDQWMRLASSVLARTSNSAAIITAPHATKAKSKHIQLISTQGRLVL
                     LILVLMDGTIKQEMLTLNDPLSQADLDESSARLNLLIHDRTSEEIAAKCSELSALDED
                     VAVRVIDIMQRTDEWSAADVYRDGLSDIMRKPEFEDRESAEGLLKVFEERSLLEDVLS
                     TALNSSVSGVQVVIGSEGRWAELHDCSLVLARYGIPDRATGALGVLGPTRMPYGRTIS
                     AVRYVSVLMTNLISEIY"
     gene            complement(3863..>5253)
                     /locus_tag="VFF70_11635"
     CDS             complement(3863..>5253)
                     /locus_tag="VFF70_11635"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HZY45390.1"
                     /translation="LFVIGLMITDAAMIALAFLLAYTIRFQISLSDPTVKIGSYRDYI
                     GLLIVQVVTIVIVAFWSRRYHLIRAVSRIDEFYAMFGIVSIGTMMSVAVSTLLFKNIE
                     LDLPRALIIYAWMLTIVLMTLGRILYHSIQARLQGRGVGRDRALIVGTGDIGRLILEK
                     IKSTPSLGYEVIGFVTKNGELDSAESLGAPVLGSANDLPDLIDNLQIDEVIIALPEAS
                     HTEILDLISKCSRASLSIKVFPDVFQIIANQVTIDDLGGLPLLSIRDVRLRGWRVTVK
                     RLVDLLITSSLLVILSPFMFLVGILIKIESPGSVFYAQERMGLDARPFLMIKFRSMRK
                     DAEANGPGWTTPDDPRRTRLGRLLRRLNVDELPQLINVLLGDMSLVGPRPERPIYVEQ
                     FKRTIPRYMERHNEKAGLTGWAQVNGLRGDTSIEERTKYDLWYIENWSLLLDFKILIR
                     TIFRTFTDRSAY"
BASE COUNT         1114 a         1490 c         1412 g         1237 t
ORIGIN      
        1 ttgtcatttg tcattgttac ttagttctta ctgaactctc cgtcgatcac atcgccgtcg
       61 gggccggcac tacccgtcgg ggcggtcggg ccggaggatg atccctcatc accagtcggc
      121 ggaggagtgg cgccgccggg ctgctcatac atgatcgatc cgatcttctg aatcgcctgc
      181 gagagttctt cggtcttgtg cttgagctct tcgacactcg cactttcgag cacgcttttc
      241 aaagcggtag ctttgctttc aatgtcggtc ttcgaatcgg ccgggatttt ttcaccgaag
      301 tcgcgcagcg atttttcagc ggtatacacc atattgtccg cactgttgcg cacttcaatg
      361 cgttccttgc gcttggcgtc ttcgcccgca aacttttcgg cgtcctttac catccgattg
      421 acttcatcgt ccgacaagcc cgacgaggct gtgattgtga tgtgctgagt tcgcccggtg
      481 gctttatcgg acgcagagac attcaaaatt ccattcgcat ccagatcgaa cgtcacttcc
      541 acttgcggcg tgccgcgcgg ggcgggcgga attccatcga gaataaattt gcccagcgac
      601 ttgttatcgg tggccatcgg ccgctcgccc tgcatcgcgt gaatctccac gctcgtttgt
      661 ccatctgccg cagttgaaaa gacctgactc ttcttcgtcg gaatcgtcgt gttgcgttgg
      721 atcaacgcgg ttgctacccc gcccagtgtt tcaatgctca gcgtcaatgg cgtcacatcc
      781 agcagcagga catctttcac ttcgccgccc aacacgcccg cttgaatcgc cgcgccgatc
      841 gccaccactt catcgggatt cacacccttg tgcggttcgc gctggaaaaa ttttcgcacg
      901 gcttcttgca ccgcgggcat gcgggtcatg ccgccgacta aaacgacttc ggcgatcttg
      961 tcgggcgaca tgctcgcgtc ttcgagcgcg cgcttgcagg gtgcgatcga tcgttcgatt
     1021 aaatcggccg tgagctgctc caatttcgat cgggttagct tcagctgcaa atgtttcgga
     1081 ccacttgcat cggccgtgat aaacggcaga ttgatttcgg tttccatcaa actcgataat
     1141 tcgatcttgg ctttttcgga cgactctttc aggcgctgca aagcctgtcg atcgccgcgc
     1201 agatcaattc cctgctcgcg cttgaactca tcggcgatgt agtcgatgat gcgttgatcg
     1261 aaatcgtcgc cgccgaggaa ggtgtcaccg ttggttgact ttacttcgaa tacgccgtca
     1321 cccacatcga ggatcgaaat atcgaacgtg ccgccgccca agtcatacac ggcgataact
     1381 tcatctttct tttgatcgag accataggct aacgaagaag cggtgggttc gttgatgata
     1441 cgcaagactt ccagacccgc gatcttgccc gcgtctttcg tcgcctgacg ctgcgagtcg
     1501 ttgaagtagg ccggcaccgt gatcaccgct tgtgtcacgg tttcacccag ataggcttct
     1561 gcgtcttgtt tcatcttctg caagatcatc gccgagactt ccggcggcga ataatctttg
     1621 tcatccattg tcacgcggca gtcgccgttg ggagcttcgg tcactttata cggcaagcgc
     1681 ttcaaagcct tctgcacttg atcgtctttg aatttgcggc ccatcaaccg tttgatcgag
     1741 tagaccgtgt tctcagcatt ggtgatggct tgacggcgcg ccacctgacc cacgctgcgc
     1801 tcatgcgttt tgggattgac cgccacgacc gatgggatca agcgtgatcc ttcggcagac
     1861 ggaatgacta ccggctcgcc gccttccatc accgcgacga cggagttggt ggtgcctaaa
     1921 tcgatgccaa taattttgct cataattttc gttgtctcct aatatatttt cgcaggtgcg
     1981 aacctacgtg tttgccaagg ctgacacaca ggtcggcccc tacttagcta ctctcactag
     2041 agccggacgc agcacgcgct cgccgattgt gtaaccatgc tgcacttcgg cgatgacatg
     2101 cccgctggct acttgttccg attcttcatg cgtcaccgct tcgtgaatag cggtatcgaa
     2161 ttcatcaccg ggctgcacgg gaatgatctt cacgcccgat tgatccagca ggcccgatag
     2221 tttccgctgg atcagcagca cgccgttgac ccacgaattg tcctggagat cttccggcag
     2281 ggtctgcacc gcgcgatcga agtcgtcgac agccggcagc actcgcagca agatatcgcc
     2341 gctgattgag ttaaacattt cggtgcgttc cagctcgacg cggcgcttgt aattgatgaa
     2401 gtccgcttta gcgcgctgcc agccttccag attctcggcg gcctgcgcct gcgcttgctt
     2461 gatctgctcg ttcaaatctt taaatagatc gatcggctgc tcggccgcat catccggcgc
     2521 aggttcgatc ggttcgtctt tgatgacatc atcactcaca gctaacttct ccattgccgt
     2581 gtcattgcga gcgcttgtgc gaagcaatct caaattgatc agattgatct ggagattgct
     2641 tcgtcgcatc cagcccagta gtgctggatg ctccacgtaa tgacgctagc ttaataaatt
     2701 tccgaaatca aattcgtcat cagcacggag acataccgca ccgcggagat cgtccggcca
     2761 tacggcatgc gcgtcggccc caacacgccc aaagcccccg tcgctcgatc gggtatgccg
     2821 tagcgcgcca agaccaaact acaatcgtgc agttccgccc agcggccttc actgccgatg
     2881 acaacctgca cgccgctcac ggatgaattc agcgcggtgc tcagcacatc ttccaacagc
     2941 gatcgctctt caaaaacttt cagcagccct tcggccgatt cgcgatcttc gaattctggc
     3001 ttgcgcatga tgtcgctcag cccgtcgcgg taaacatcgg ctgccgacca ttcatcggta
     3061 cgctgcatga tgtcgatcac gcgcacggca acatcctcgt cgagcgcaga cagctcgctg
     3121 cacttggccg cgatctcttc agaagtgcga tcgtgaatca gtaagttcaa tcgtgcagaa
     3181 gattcatcga gatcagcttg gcttaacgga tcgttgagcg tgagcatttc ctgtttgatt
     3241 gtgccatcca tcagcaccaa aatgagtaag accaatcgac cttgcgtgga gatcaactgg
     3301 atgtgtttgc ttttggcttt tgtggcgtgc ggcgcggtga tgatggccgc gctattcgac
     3361 gtgcgtgcca agaccgatga agccagccgc atccattgat cgagatcgag ccgggcctga
     3421 tggaactgat gatcgatcgt gcgctgctcg ttcgccggca gttcgtcatc gtccatcagc
     3481 acttcgacga agtaacgata acctgaatcg gtgggaatgc gtcccgccga agtgtgcgga
     3541 tgcatgagat agccgtgctg ctccaagtaa gccagttcac tgcgaatagt ggcaggcgaa
     3601 taatcgaggt tgtaacgctc gactaaaact ttggagccga ccggctgcac gcgatggaca
     3661 ccgtcttcgc tggctgtctc gctgctgacg tattcgcgca cgacgaggcc caatataagt
     3721 tgttgacgtg aagttaattc gtccataggg aattcaaggt tattacaacg attagcactc
     3781 tcgggccgag agtgctaatt actctgtgat tgattatacc gtctctgaaa gggtgtgtca
     3841 agaaaaactt aatgcgcaga gtttaatacg cgctgcgatc ggtgaaggtg cggaagatgg
     3901 tacggatcaa aatcttgaag tcgagcaaca gcgaccaatt ctcgatgtac cacagatcgt
     3961 atttggtgcg ctcttcgatc gaggtgtcac cacgcaggcc gttgacttgc gcccaaccgg
     4021 tgaggccggc tttctcgttg tggcgttcca tatagcgtgg aatggtgcgc ttgaattgct
     4081 cgacgtagat cggccgctcc gggcgcggcc cgaccaaact catgtcgccc aacagcacat
     4141 tgatcagctg cggcagttca tccacattca agcgacggag caagcggccc aatcgggtgc
     4201 ggcgaggatc atcgggtgtg gtccagcccg ggccgttcgc ttcggcatcc ttccgcatcg
     4261 atcgaaattt gatcatcaag aagggccgcg catccaggcc catgcgctcc tgcgcgtaaa
     4321 acaccgagcc gggcgattcg atcttgatca agatgccgac cagaaacatg aagggcgaca
     4381 ggataactaa caggctgctg gtgatgagga gatcgaccaa gcgtttgacg gtgacgcgcc
     4441 agccgcgcag ccgcacgtca cgaatgctca acagcggcag gccgcccaga tcgtcgatgg
     4501 tgacttgatt ggcgatgatc tggaagacat ccgggaaaac cttgatgctg agcgaggcgc
     4561 gcgaacattt tgagatcaaa tccagaatct cggtgtgcga tgcttccggc agggcgatga
     4621 tcacctcatc gatttggaga ttgtcgatca ggtcaggcag atcgttggcc gagccgagca
     4681 ccggcgcgcc taaactttca gctgaatcca gctcgccgtt cttggtaaca aacccgatga
     4741 cttcgtagcc caaactgggt gtgctcttga tcttttcaag aattaagcgt ccgatgtcgc
     4801 ccgtgccgac aatcaaggcc cgatcgcgcc cgacgccacg gccttgcagc cgcgcctgga
     4861 tcgagtgata gaggatgcgg cccagcgtca tcagcacaat ggtcaacatc cacgcgtaga
     4921 taatcaacgc gcgcggaaga tcgagttcga tatttttgaa gagcaaagtc gagacggcca
     4981 cggacatcat cgtgccgatc gagacgatgc cgaacatggc gtagaattcg tcgatgcgcg
     5041 acacggcacg gattaagtgg tagcgccgcg accagaaagc gacgatcaca atcgtcacga
     5101 cttgcacgat caacaggcca atatagtcac gatacgatcc gatcttgacg gtgggatcag
     5161 atagggaaat ctgaaagcgg atggtatagg ctagtaagaa cgctagcgcg atcatcgcgg
     5221 cgtcagtgat catcaaaccg atgacgaata agg
//
LOCUS       DASRTJ010000573         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_74057,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000573 DASRTJ010000000
VERSION     DASRTJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74057"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFF70_11640"
     CDS             <1..614
                     /locus_tag="VFF70_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY45391.1"
                     /translation="SELLLTDNHDLPETAAQESLQHILYSGKKMDRIIEELMLLTGVR
                     QQEIVPAPIDIGKTVNDALSSLSNVIQDAGAQISVPDQATWPQAVGYAPWIEQVWVNY
                     LSNALKYGGQKPQIELGATAQSTDTIRFWVRDHGNGLTSEEQSRLFTAFTRLNQIRVT
                     GHGLGLSIVRRIVEKLGGTVGVESQPGRGSTFFFTLPIALHDN"
     gene            complement(688..>1967)
                     /locus_tag="VFF70_11645"
     CDS             complement(688..>1967)
                     /locus_tag="VFF70_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45392.1"
                     /translation="LFPDRMTIHDNSNLESTTRRLTTVLFIAQGLALLAIFTTSAISS
                     ITGTLLGGTARVAGWPSAATLIGGSLAAYPIGRLMGHVGRRIGLSIGYLIGMLGGIVA
                     GLGVIGQSLSVFLIGSMLLGAARVSSDQARYAAADVSTLRNRARAVSLIVFAGTIGAV
                     LGPALVPIADRAGQAIGRGQLVGPWLICGALFVIPLIVINIFLRPDPRDVAYQLSATN
                     ALGQETIILAPARSLLQVLADPAARVALIALGLAQIVMVMVMTITPVHMLDYAHPLED
                     ISLVISAHIVGMYGLSMVTGWLSDRVGRRAVIGLGSTLLIGACVFAPVNHDTLPLAAS
                     LFLLGLGWNCCFVAGSALLTDRLLLHERARVQGTAELVVSVASAIGSLGSGELMARVG
                     FASATTIGMGLALVILAASIARIAVRRANESIA"
BASE COUNT          490 a          596 c          532 g          349 t
ORIGIN      
        1 gtagtgaatt gttgctcaca gacaatcacg atctgccaga gaccgcagcg caggaatcac
       61 ttcaacatat tctgtacagc ggaaaaaaga tggatcggat tattgaagag ttgatgctgc
      121 taaccggcgt gcgtcagcag gagattgtgc ccgcgccgat cgatattggc aaaactgtca
      181 acgatgcttt aagcagttta agcaatgtga ttcaagatgc cggcgctcag atcagtgtac
      241 ccgatcaagc aacttggccg caggctgttg gttatgcacc atggatcgag caagtttggg
      301 tcaactatct cagtaatgcg cttaaatacg gcggacaaaa accgcagatc gagttgggcg
      361 caaccgctca gtccacggac acaatccgtt tctgggttcg cgaccatggc aatggcctca
      421 cctccgaaga acaatccaga ttgttcaccg ccttcacgcg cttgaatcaa attcgggtca
      481 caggccacgg cttgggtctt tcgatcgtgc ggcgaattgt cgagaaactt ggcggcacgg
      541 tcggcgttga aagccagccc ggacgtggca gcactttttt cttcacactg ccgatcgcgc
      601 tgcatgataa ttgatcgcaa gacgcactat ccacgcaacg aggcgacttt attgtagtcg
      661 cctcgattca ttgcaatcac ttacatctca tgcgatcgat tcgttcgcgc gcctcaccgc
      721 gatccgcgcg atggatgcgg ccaaaataac cagcgctagc cccataccaa tcgtcgtggc
      781 tgaagcaaaa cccacgcgcg ccatcaactc accgctgccc aaactgccga tcgccgacgc
      841 gacgctcacc accaactccg ccgtgccttg gacccgcgcg cgctcgtgaa gcagcaatcg
      901 atcggtgagc agtgccgagc cggcgacgaa gcagcagttc caacccaagc ccaacaaaaa
      961 caacgaagcc gccaatggca gcgtgtcatg attcacgggc gcaaagacgc acgcgccgat
     1021 caacagcgtg ctgcccaaac caatcaccgc gcggcgcccc acccgatcgc tcagccagcc
     1081 ggtgaccatc gataaaccgt acatccccac aatgtgcgcc gagatcacca gactgatgtc
     1141 ttccagtgga tgcgcgtaat cgagcatgtg aaccggtgtg atcgtcatca ccatcaccat
     1201 gacgatctga gctaagccta gcgcgatcaa agccacgcgc gccgcagggt cagctaaaac
     1261 ctgtaagagc gagcgggcgg gtgcgagaat gatagtttct tgacctaatg catttgtggc
     1321 cgataactga tatgcgacat cgcgcggatc gggccgcaga aaaatattaa tgacgatcag
     1381 cgggatcacg aacagcgcac cgcagatcaa ccacggcccg acgagctggc cacgcccgat
     1441 cgcttgacca gcccgatcgg cgatcgggac cagcgccggg cccaacaccg caccaatcgt
     1501 tccggcaaaa acgatcagac ttaccgcgcg cgcacggttg cgcaacgtgg acacatcggc
     1561 cgccgcatag cgtgcttgat cggatgaaac gcgcgccgcg cccaacaaca tcgagccgat
     1621 taagaacaca gacagtgatt ggccgatcac gccgagaccg gcgacgatgc cgcccagcat
     1681 gccgattaga taaccgatcg acaaaccgat gcgtcgaccg acgtggccca tcaaacgacc
     1741 gatcggatac gcggcgagcg acccgccgat caaagtggct gccgatggcc agcctgccac
     1801 acgcgctgtg ccgcccaaca gtgtgcccgt gatgctggag atcgccgatg tagtgaagat
     1861 cgccagcagc gccaagcctt gagcgataaa cagcacggtc gtcaatcggc gcgtggtcga
     1921 ttcgagattt gagttgtcgt ggatggtcat gcgatcaggg aataagg
//
LOCUS       DASRTJ010000574         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_35977,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000574 DASRTJ010000000
VERSION     DASRTJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35977"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VFF70_11650"
     CDS             complement(<1..1490)
                     /locus_tag="VFF70_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZY45393.1"
                     /translation="MPRAETITHDVIVVGAGLAGTWAAMVAGQQGVKDVAVLSKIHPL
                     RSHSGAAQGGIAAALGNTRPVKGSGAEGPLEQVPPGDEASDSLDSHIFDTIKGSDWLG
                     DQDAIATMVNDAPRVIYEYEHMGCVFSRTPDGRIDQRRFGGHSAPRACFAADWTGHVL
                     LHTIHEQALRHGVKFYSEWYAMDLIIEDNICRGVVAMDILTGEIHTMRAKAVMFGTGG
                     YGRAYKITSNAYANTGDGVAIAYNAGVPLMDMEFVQFHPTGLYEHGILMSEACRGEGG
                     HLLNKDGERFMQRYAPDKMELGPRDLVSRSEQREINEGRGGGADGQAIFLDLRHLGRE
                     KILQRLPQVRELAIDFVGVDPIDSLVPIQPTAHYSMGGIPTNSSGQVITDADGTPLTG
                     LYAAGECACISVHGANRLGTNSLLDASVFGRRAGYAVAEFIHGGAKLFPVSGDPAAKN
                     QQRLQHFFDNPGKESVEQIAQELKTTMTSNCGVFRDNDHLQIALRD"
     gene            1853..2005
                     /locus_tag="VFF70_11655"
     CDS             1853..2005
                     /locus_tag="VFF70_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45394.1"
                     /translation="MSVELIRVDDDSRATVDFAVDYVLKPNGCLPLVARDDAEGVYEA
                     LTARPI"
     gene            2244..>2683
                     /locus_tag="VFF70_11660"
     CDS             2244..>2683
                     /locus_tag="VFF70_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45395.1"
                     /translation="MSVSNQSARHWLSWAAIITALITLLALPVQAAPLNQDAQATITT
                     PTDGQVVNGNVLISGSAAHPDFDRYEIAYGPDPNPNDAWQIFVNNNQMVAANTLGTWN
                     TSVLVDGTYIIRLRVIRRDSNYTEAFVRGIKVSNQQPVGTPTSVP"
BASE COUNT          625 a          763 c          662 g          633 t
ORIGIN      
        1 atgtcgcgca gcgcaatttg tagatgatcg ttatcgcgaa agacgccgca attgctggtc
       61 atcgtggtct tcaattcttg tgcgatctgc tcaacggatt ctttgccggg attgtcgaag
      121 aagtgctgga ggcgctgctg attcttggcg gcaggatcac cgctgacagg aaataatttc
      181 gcgccgccgt gaataaattc tgcgacggca taacccgcgc gccgcccgaa gacggaggca
      241 tctaataggc tgttcgtccc caatcggttc gcaccatgca cactgatgca ggcacattca
      301 ccggccgcgt acaagccggt cagcggcgtg ccgtccgcat cggtaatcac ttgaccgctc
      361 gaattggtcg ggataccacc catactgtag tgcgcggtcg gctgaatcgg gacgagtgaa
      421 tcgatcgggt ccacgccaac aaaatcgatc gcgagttcgc gcacttgtgg caaacgctgc
      481 aaaattttct cgcggccaag gtgccgcaga tcgagaaaga tagcttgccc gtccgctcca
      541 ccaccgcgcc cctcattgat ttcacgctgc tccgatcggc tgaccagatc acgcgggcca
      601 agttccatct tgtcgggcgc atagcgctgc atgaaacgtt cgccatcttt gttgaggaga
      661 tggccgcctt caccgcgaca ggcttcgctc atgagaatgc cgtgctcata taagccggtg
      721 gggtggaatt gcacaaattc catatccatg agcggcacac cggcgttgta agcgatcgcg
      781 acaccatcgc ccgtgttggc atatgcattc gaagtaatct tataggcacg gccataaccg
      841 ccggtgccaa acatgactgc cttggcacgc atggtatgta tctcaccggt caggatgtcc
      901 atcgcgacga cgccgcggca gatgttgtcc tcgatgatca aatccatcgc gtaccattca
      961 ctgtaaaact tcacgccatg acgcagggct tgttcatgaa tcgtgtgcag caagacgtga
     1021 ccggtccaat cggcggcgaa acatgcgcgc ggcgcactat ggccgccaaa ccggcgttga
     1081 tcgatccggc catcgggcgt gcggctgaac acgcagccca tatgctcata ttcgtagatc
     1141 acccgcggcg catcattcac catggtcgca atcgcatctt gatcgccgag ccaatccgaa
     1201 cctttgatcg tatcgaagat gtgtgaatca aggctatcgc tggcctcatc gccgggtggc
     1261 acttgctcca gcggaccttc agccccacta cctttaaccg gtcgcgtatt gcctaaggcc
     1321 gctgcgattc ccccttgggc agcgccacta tgcgatcgca acggatgaat cttggacagt
     1381 acggccacat ctttaacgcc ttgctgtccg gccaccatgg ccgcccacgt cccggctaaa
     1441 cccgcaccga cgacaatgac atcatgcgtg attgtttctg ctcgtggcat agatttcctt
     1501 tcctcgataa tgccctgagt cgttgttata aaaaagacgg ccttcgcgct atactagtta
     1561 gcacaaccaa ccgccccgct agcagatttg tgcacatcaa tttatcactt ttatcaacac
     1621 ctaaatggta gactggcaaa ccagcagcgg ctagttgtaa acgtcacgca attttacgcg
     1681 gtctatcagg taacacctac catgactaat ttacttcgct aggtacgcat tcagcctcac
     1741 atgtcccaat acgcttgaac agtaactgga tcataactcc gtgttccgtc gaattttgat
     1801 acaattcacc taaggaaata cgtgcaacat aatcgctatt gaaaaataaa ccatgtcagt
     1861 cgaactaatt cgtgtcgatg acgattcaag agccacggta gattttgccg tcgattacgt
     1921 cctcaagccc aacggctgtc tgccacttgt cgcccgcgat gacgctgaag gtgtgtatga
     1981 agccttgacc gcacgtccga tttgatcatc ctcgattttg aaatgcccga gatgaacggc
     2041 ccgatcgatg tgaaaagatt gcggttgtcc gtcgaagcgt ttgaagtgct taatctcaga
     2101 aaatcaatgt ctttctaaaa ttttatgcgt gagctttcac gtccgccttc gtgcagacgt
     2161 tttcattttt caacattatc tttgtcgaag gccttcgagt atgttacaat taactgttga
     2221 aaacttcatt gtttgaggcc aagatgtcag tatcaaacca gagtgcgcgc cactggctca
     2281 gctgggccgc aatcattacc gcactcatta ctttattagc gctgccagtc caagccgcgc
     2341 cgttgaatca agacgcacag gcaaccatca ctacgccgac tgatggccaa gtcgtaaatg
     2401 gcaatgtctt gattagcggt tcggccgctc atcctgactt cgatcggtac gagatcgctt
     2461 acgggcctga cccgaacccg aacgatgcgt ggcagatttt cgtcaacaac aatcagatgg
     2521 ttgcggccaa cacgttaggc acgtggaata cttccgtgct cgttgatggg acgtatatca
     2581 ttcgactgcg cgtcattcgg cgggacagca attacactga agccttcgtg cgcggcatta
     2641 aggtgagcaa ccaacaaccg gtcggcacgc caacatctgt ccc
//
LOCUS       DASRTJ010000575         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_74135,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000575 DASRTJ010000000
VERSION     DASRTJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74135"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..776
                     /locus_tag="VFF70_11665"
     CDS             195..776
                     /locus_tag="VFF70_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HZY45396.1"
                     /translation="MSIPIQPNLAHIDLSTGVIAEAVEITERHLRDMPGFFQDQQTVR
                     ELLPNDPLMYRVYLTHQGGDTNGLYTGTSVIEPGRIGREYYMTKGHFHLANTAPEVYL
                     TLSGQGLLLMQTHSGEIAIQPMKPGAMNYIPGEWAHRTINTGEVQLVFFAVWPVSAGH
                     DYDSIAHHGFAQLVMAGLEGPEVVANPNFTPSR"
     gene            784..1737
                     /locus_tag="VFF70_11670"
     CDS             784..1737
                     /locus_tag="VFF70_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZY45397.1"
                     /translation="MGKCVLVTPRSFAALSDEPLRLLHAAGCEVIQLDRPADGCVTED
                     VMCAAIKGMDAVIVGLEPLSVRVLEAAHKLRVISKYGVGLDNIAVAAARARGIAIGWT
                     PDANSNAVAELTLGLMFALARRIPAASAQTRVGDFSKVVGVELRGRTLGIIGLGRIGQ
                     RVALGAIGLGLRVIAYDIRSGLATDPIEPVDLDTLYAQADIISLHIPLTTETAGLIDA
                     AALSKMKRGALLINTARGGLIDEAALYPALQSGQLSGAAIDSFAHEPPIDHPLLTLNN
                     FIGTPHIASHTTEAIACMGRIAAENVIAGLCGEPLPYPYSH"
     gene            1767..2048
                     /locus_tag="VFF70_11675"
     CDS             1767..2048
                     /locus_tag="VFF70_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZY45398.1"
                     /translation="MTTSNELVIRNKVGLHARPAAQFVKTASQFKSKASVENLSKGTP
                     PANAKSILSVLSIAVQLNDRIRLTIEGIDEAAALAALCGLIENNFGEAE"
     gene            2056..>2727
                     /locus_tag="VFF70_11680"
     CDS             2056..>2727
                     /locus_tag="VFF70_11680"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HZY45399.1"
                     /translation="MSVQTLHGISASPGIAIGPVYLYAVQHVVVARRSGFDVDNELDR
                     LASALTAARQQLAALTERARQAAGADEAAVFEAHQMFLDDPELLDRVRTLLRKESVSA
                     EYAWQAGTHHYAETLSRIDDEYLAARAADVKDVAQRVLRILVGVHSEVGGPQTPAVIA
                     AEDLTPSDTVTLDKSKVLAFCTAGGGPTSHVAILSKALGIPAVVGLSDSFSALKNART
                     VIVDGG"
BASE COUNT          562 a          751 c          825 g          589 t
ORIGIN      
        1 ccttattaca ccgaagtagc ccgaccgggc ggcaacccgg atgtgcagcg aactgatcag
       61 ccacgttacg cgcgggcgtt cgacatcgcc tacccgccga agtcgcattg atcggtctgg
      121 atagatcggg gtactgaatt cattgtgagc aagttttatc tttaggtgta attgagctag
      181 ggagcattga tcgcatgagc attcccattc agccgaatct ggcgcacatc gatttgagta
      241 ccggggtcat cgctgaagcc gtagaaatta ccgagcgcca cttgagggac atgccgggct
      301 tcttccaaga tcaacagacg gtgcgtgaat tgttgccgaa cgatccgctg atgtatcgcg
      361 tttacctgac ccatcagggt ggcgatacca acggcttgta caccggaacg tcagtaatcg
      421 aaccgggcag gattggtcgc gaatactata tgaccaaggg ccatttccat ttggccaata
      481 ctgcaccgga ggtctatttg acgctgagtg gacagggtct gttgttgatg cagacccact
      541 ctggtgagat cgcgattcaa cccatgaagc ccggcgcgat gaattacatt cccggcgaat
      601 gggcgcatcg aacgatcaac accggtgagg tgcagttggt cttcttcgcc gtttggccgg
      661 tgagtgccgg gcatgactat gacagtattg cgcaccacgg ctttgcgcag ttggtcatgg
      721 cggggttaga gggtccggag gttgtagcga atccaaactt tacacccagc cgctgactga
      781 gtcatgggca agtgtgtatt ggtcacaccc cgatcgtttg cggccctatc cgatgagccg
      841 ttgcgtttac tgcacgcggc ggggtgcgag gtgatccagc tcgatcggcc agcggatggc
      901 tgcgtcactg aagacgtcat gtgcgcagcg ataaagggta tggatgccgt tatcgtgggc
      961 ctcgagccgc tcagcgtccg tgtgcttgaa gctgcacaca aattgcgcgt gatttcgaaa
     1021 tatggcgtcg ggctggataa cattgcagtc gcggcggccc gcgctcgcgg tatcgcgatc
     1081 ggttggacgc ccgatgcgaa cagtaatgcc gtggctgagc tgaccctggg tttgatgttt
     1141 gcgctggccc gtcggattcc ggctgcttca gcccagacgc gagtcggcga tttctcgaaa
     1201 gttgttggcg ttgagttgcg cggccgaacg ttgggcatca tcggactggg acggatcgga
     1261 cagcgggtag cacttggtgc gatcggctta ggcctgcgcg tgatcgctta tgatattcga
     1321 tcgggtctgg ccactgatcc gatcgagcca gtcgatctcg acacactgta cgcacaggct
     1381 gatatcatct cgttgcacat cccgttgacc acggagacag ccggattgat cgatgcggca
     1441 gctctgtcta aaatgaaacg cggtgcattg ctgatcaaca cggcgcgcgg cggtctgatc
     1501 gatgaagcgg ccttgtatcc tgcccttcaa tccggtcagc taagtggtgc ggcgattgat
     1561 tcgtttgccc acgaaccgcc gatcgatcat ccgctgctca cgctgaataa cttcattggc
     1621 acgccgcaca tcgcatcgca cacaacggag gctatcgcct gcatggggcg catcgccgcc
     1681 gaaaacgtca tcgccgggct gtgcggtgaa ccgctgcctt atccgtatag tcattagcga
     1741 tcgagaattg atgaaggaga aagggattga ctacctctaa cgagctggtt attcgcaaca
     1801 aagtcggtct gcatgctcga ccggccgctc aattcgtcaa gacggccagt cagttcaaat
     1861 caaaggcctc ggtcgagaat ctcagtaaag gcaccccgcc ggcaaatgcc aaaagcatcc
     1921 tgagtgtgct gtcgatcgcg gtgcagctga acgatcggat ccggctgacg atcgaaggca
     1981 tcgacgaagc cgccgcgctg gccgcactgt gtggcttgat cgaaaataat ttcggcgagg
     2041 cggagtagac cggccatgtc cgtccagaca ctgcacggca tcagtgcttc gcccggcatc
     2101 gcgatcggcc cggtgtatct gtatgcggtt caacacgttg tcgtcgcacg acgctccggc
     2161 ttcgatgtgg acaacgaact cgatcgctta gcatcggctt tgaccgccgc ccgccagcaa
     2221 ttggcagcgc tgaccgagcg ggcacgtcaa gccgcgggcg ccgacgaagc cgccgtgttt
     2281 gaagcgcatc agatgttttt ggacgatcct gaattgctcg atcgagtccg gacgctcctg
     2341 cgcaaagaat cggtcagtgc cgaatatgcc tggcaggcag gcacgcatca ttacgccgag
     2401 acattaagcc gcatcgacga tgaatatctg gccgcgcgcg cggccgatgt gaaagatgtc
     2461 gctcaacgcg tgctgcgcat tttagtcggc gtccattcgg aagtaggcgg cccgcaaaca
     2521 ccggcggtga tcgcggccga agatctcacc ccctccgata cggtaacgct tgacaagtcg
     2581 aaagtcctgg cgttctgcac tgcaggcggc ggcccgactt cgcatgtggc aattttgtcg
     2641 aaagcgttgg gcattcccgc cgtggtcggt ttaagtgata gtttcagtgc gttgaaaaac
     2701 gcccggacgg tgatcgtcga cggcggc
//
LOCUS       DASRTJ010000576        17552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_355973,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000576 DASRTJ010000000
VERSION     DASRTJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 17552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17552
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355973"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFF70_11685"
     CDS             complement(<1..450)
                     /locus_tag="VFF70_11685"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HZY45400.1"
                     /translation="MQLLRRIVITIVMLLLFSSLAGGALAAPLVQQSNGQVCVQAFDD
                     TNGNAARDADEKALAGVGFTLSDDSGVKGSYSTDGVSEPYCFGKLAAGSYTVRAKAPV
                     GYESTTEGQWAISLASGAQFDAKYGAQKIGGDSSTPGNPTSGNTSAPS"
     gene            complement(481..1953)
                     /locus_tag="VFF70_11690"
     CDS             complement(481..1953)
                     /locus_tag="VFF70_11690"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45401.1"
                     /translation="MTPRKELALAGVFALALMMLFNLMPNSAHAARLSQSGNLLQSPG
                     MDQPYNGCGAGDTASGWSCWYRQIDKPGDASALTYTIRPQFGAESNPSGAHPELIHGD
                     PTSQHIGYQQDPWMAGIKQTVPNIPAGSVVQFCAYSRIRASNSPVGTGSDGDVQGRAR
                     VGIDPNGGDNWASGVTWSGAANPHDTWQQLCVQATVGSAGKVTVFTSNDFRQYTRTHL
                     DAWWDDAALTVVGAATPAPGATSAPQQQPTSAPPPAPLPSVISTPNPDGSIVHTIQSG
                     DTLFALSIAYDVPLDQILTLNNITKDALLSIGQKIILRGPTGPALPTAAPTSTTPEAP
                     PTAAPTATPEVITAKLCVQAFDDTNADGLLTTGEQSVAGVQFTVANSQSGEQVSTYTS
                     DGSAAPHCFDNLVPGTYSVAVQPAPNTVATSDKRWSVSLTGGSTVNIRFGSRADASPT
                     PIPATGSSSSLSGLLVGGIGLVVLLVAGVLGAFVIARRRA"
     gene            complement(2043..3566)
                     /locus_tag="VFF70_11695"
     CDS             complement(2043..3566)
                     /locus_tag="VFF70_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45402.1"
                     /translation="MKPQRISIAIAMVCLAWMSSTIATAQVQPARLDQSSRNLLTNPG
                     FEGDYQIQCSFPGGRPWMTLPCDGSLPTVPWQTVQVADGWHGWWQIPSTDRQAPDYYR
                     YPNYCGRNAPDDCVAWHNPEFRDTRSSPQDPPRIRSGENSQKYFTFWSVHEAGVYQTV
                     TSVRPGSLLRFSIYMEVWSATRLSEGEPNPHFSFGQGSMHLRVGIDPTGGNDPWSKDI
                     VWSTEHEAYDQFTLFEVEAVSRSSKVTVFTHSRPEYPMEHNDVYADDAELTLVGGGAS
                     VPIVVNPPPVVVAVGAQPTAVPGRTGKLTHIVKSGDTLFAIALQYGVPVDQIMQLNGL
                     SAESQLALGRELVISLPPISVAPRPISSIGGPGSGLGTICVRAFNDVDGDGFRSISDQ
                     ALSAAGLQIDVIDGNGNPIVARTLDGSADQACFDHLPATVYQVAAQTPSGYSVSGSRR
                     WAVALNSDATVDIALGLHIDPASVVIPPELLVGASVLIAATAVFIWRRRQSQPVKYF"
     gene            complement(3580..5844)
                     /locus_tag="VFF70_11700"
     CDS             complement(3580..5844)
                     /locus_tag="VFF70_11700"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45403.1"
                     /translation="MQLRRFLILSLIFSFAVAWLPLNVQASPISAASPNLLKNPNFET
                     GFTQINADAQVATSWSAWWTPHPASAPDWAFLQPNYSPSNTCTQGCDHRIHGGGNAQR
                     MFHFFGAYEAGIYQQVAVPLNVDLQFSIYAQGWSSQQNDPINSSLGGTDMQMKIGIDP
                     TGGTDPKSPNVVWTQPRNILDSWYQFSVYARSQNTTVTVFTYANPFDSRRNNEVYWDD
                     AALSVLSGDLAATAQASYPTATPVPIVLTPTPASVQVGVNLLTDGGFEGKLYIPCSTI
                     SDVPWRHISCDGLDFDVKDEKGRRINIIWNTVQVPIGWTGWWLEPNIKHGTDPKFYSN
                     HPNNCYSDAPEGCIAWHNPEFRDTKGVVSGPSRIHSGKNSQKYFTFYSVHEAGLFQMV
                     NVLPGSLLRFGVYMHAWSSNQDGSELDPSSYNSSGQTSMHMRVGIDPTGGDDPWSPNI
                     VWSPEHDSYDIFGYYEVRATAQSDRVTVFTHSMPEKGLKHNDVYVDDAELVAIQVPDQ
                     PAGAPPVSNNISAPPAVINTAPGPTATPYPDGSVVHTVKPGDSLFGLSLQYNVPMDQI
                     MQLNGLTKDSFIVIDRELVISASIVTVTPAVTSLPTIAATPMPEQVAAAPESTQLCVR
                     AFNDVDASGVYISSEALVTGALFTLFDAQGQQLTAYSTDGISEPHCFNKLAPGNYSVS
                     IQAPTGMIATSDRRWSVTLGSGATVNVNFGSRPSESKPAAPSDAMGMIGLLIIGALIG
                     IGGWIVLLRRKTIA"
     gene            complement(5938..7920)
                     /locus_tag="VFF70_11705"
     CDS             complement(5938..7920)
                     /locus_tag="VFF70_11705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45404.1"
                     /translation="MRRILNEWAPVIGLMLLAGALRLYMLGAWPPGLYHDEAFNGLDA
                     LRVLGGDHPLYFGANNGREPFFIYLVSLSVSLFGRSVIALRLPAALIGTLTVPATYWL
                     AHELFNRRVGVLSAAIMAVTLWPIHLSHIGLRAVSLPLFIALFIAMGIRAYRSNRRRD
                     WIIAGLLYGASFYTYLASRFTPAVLIVFSIVLIAVRRSRRVWPRVIFFLAAAALVIAP
                     LAITAVNEWDVVMGRPGEISIFNPIINHGDFVGTAVSSTLKALGMFFWQGDRIPRHNV
                     PYRPVFDPIMAIFFVVGLICLVCAVLPQRTPRAHSHSNFFLAINKLSSLFTLLWVAVM
                     SLPTILAEDPPHFLRVVGILPLVMIIPAIGLDVVGRWIAARSKLIGIGVMSAAIIMAG
                     VLTVRDYSQYAIDPETANAFEVAGTELARVAQADIISNQQVFIADRFIRDWTSVSFLL
                     NGDYQTLSDGGQASIDPARSATLFVWPYEDFSKSFANLPGPLMLQVSAGPMAQGDLDP
                     QPHVGYLRVQIDPKQSTSNITEALFDNGLRLLGHSIEVIDDGHWRLRTLWTSEQPQSN
                     DQTFFVHLLLNNQLVVSVDGDSGDNFYPASLWRVGDVVIDQRTIELPAQADRVQLLIE
                     MGVYSRSTGQRVKVLQSAQAVIDQAILLGGPNAVGP"
     gene            8046..9203
                     /locus_tag="VFF70_11710"
     CDS             8046..9203
                     /locus_tag="VFF70_11710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY45405.1"
                     /translation="MTLIRNRDPWSSVYGRNNMKVAILSKALIVCAYRSKLEQLAALP
                     DIEVIAIVPPEWRDERGVMRMEDSLDNVYELIVTPIAFNGNFHLHFYPQIDRLLQRIR
                     PDILHIDEEPYNLATVLALRSAQRVKARTLFFSWQNLNRSYPFPFNRIERYVLNHVDA
                     CIAGNQDAQTVWRAKGYRGPIEIIPQFGVDQDMFAPAPRSRDGTFVIGYVGRFVLEKG
                     IDILLQALSKLTGSWRADLLGSGPDQTRLIDLAQRLHIENRVRCLPWIPSTRLPDYYH
                     SLDCLVLPSRTQSNWKEQFGRALVEAMGCGVPVIGSNCGEIPNVIADAGLIFPEGNVD
                     ALRQLLINLQENPAMRADLSRRGRQRVLDQFTQAEIARKTYGVYQRIKQAA"
     gene            9349..10494
                     /locus_tag="VFF70_11715"
     CDS             9349..10494
                     /locus_tag="VFF70_11715"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY45406.1"
                     /translation="MIDQPPRIAINAHLLAPRGTGGYRSAGIHVYTEQVLRHLPQADP
                     DLRYTVFTAHPPSDLDPAIELQTSRWNTARPATRIAWEQIVQPRATRRVKAAVLHDTA
                     FVGPIVRPCPIVLTIYDLSFALYPQFFRGLKQTYLRIGTRYSAKRAKRIIAISDNTRR
                     DIARLYQVPLDRIDLAYPGVDETIGRRSVVEVERFRSEKSLPNKFLLFLGTLEPRKNL
                     PVLIRAFAQMKIDCPDAKLVLAGGFGWRTAEIFNTVKLAGVEDRVLWPGFVASEEKTL
                     WYSAATAFVYPSIYEGFGLPPLEAMACGTPIIVSNAASLPEVVGAAGISIDPQDVNSW
                     AAAMTRVWRDEAYRAELAHRGLAQAQKFRWIETARATTSSYRRALEK"
     gene            10496..10807
                     /locus_tag="VFF70_11720"
     CDS             10496..10807
                     /locus_tag="VFF70_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45407.1"
                     /translation="MDRSNRKRIVLLLILALPAIIYISIAQRQFEITGEKRIDGIIGV
                     MLGLYICSLAAADLLDMLFARWPRLSKLTQLQWLALNAAFLVLGIVVIIMGTTRFTAT
                     S"
     gene            10843..12270
                     /locus_tag="VFF70_11725"
     CDS             10843..12270
                     /locus_tag="VFF70_11725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZY45408.1"
                     /translation="MKPKYPRWLMWCVVIGDSVLINVAFLLAYTLRYDVQLLRPVEPI
                     FDNPPTVYIPFAVLLTGLLLITFKIDGVYQSRRLGMWLEQMTAIVRGTTLGILLMVGI
                     TFFYQPTFYSRLIFVYDSVLIVLLLGVGRLVWGFILAQLRRRGIGVVRVLIVGADEVG
                     RTVIRTVMAQPELGYRIVGLISDQFDTNTLNIGPIPLLGGLEKIPQAISEQRIDEVVI
                     ALPWTDQQRVLEVFQTCDRLGVRARTVPDIFQLSLNRVDVEDLGGVPLLGLKSASFRG
                     TNLLVKRLIDLIVGGLISLVALPFMLLLSIAIRINSPGAIIFRQKRVGMRGKEFTIYK
                     FRTMRAGAEEEQERLLVYNEFTGPLFKMKGDPRITQVGRFLRKTSLDELPQLFNVMKG
                     DMSLVGPRPHIASEVAQYQDWQKQVLEAPPGMTGLSQVSGRSQLSFDEQCLLDIYFIE
                     NWSPALDFKILLRTIPKVVLGEGAY"
     gene            12276..13319
                     /locus_tag="VFF70_11730"
     CDS             12276..13319
                     /locus_tag="VFF70_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45409.1"
                     /translation="MKDELDEWAISRALGRMAKYLPDRSKDTALALARNSIDPSARIR
                     ALSGLINVLPPIDHAAALQEAQTALHSLSNPWLRVRSMLILALRLSPEAKDRILREAF
                     GTTLNIPNDWVRGRALNLIYGHLPADLIDRAIEAASKLQNLDMRTVALSSMAARLSDP
                     LTSEVLRDIPSIEDESLRASALNDLARRLTPDQLKHALSIAYGLQDETARVDALSGLS
                     QQLAEPIKNKVISEMLDITQHADDGTRRAEMLLLIVTRQPQVLSSSIVAEITRAIQKT
                     IGADIRVDLFTKLAGLLPPEQRSIVLTEALSAARSIENAAARAEALSGLIGFISIDQR
                     EAIVHEVLAMLQV"
     gene            complement(13387..13776)
                     /gene="rplL"
                     /locus_tag="VFF70_11735"
     CDS             complement(13387..13776)
                     /gene="rplL"
                     /locus_tag="VFF70_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZY45410.1"
                     /translation="MSEKLDQIVAQLDALTLMEAAELSKQLQEKWGVSAAAPMMMGAM
                     PAGGGAAAAAPVEEQTEFDVILKDGGAKKIDTIKVVRQLVNTLGLKEAKELVDTAPHA
                     VLTAVSKEAAADARAKLEAAGASVEVK"
     gene            complement(13862..14392)
                     /gene="rplJ"
                     /locus_tag="VFF70_11740"
     CDS             complement(13862..14392)
                     /gene="rplJ"
                     /locus_tag="VFF70_11740"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZY45411.1"
                     /translation="MPVSKQRRSELINEYTEWLKHSQAVIIASYQGVLTKDLYQLRLK
                     IHEANGELHVVKNTLAARALKAANLPVPEELLAGSVIMGFCFQDPAVIAKAMVNFAND
                     KQSFKVKGGLLDKRLIDVAGVKALADLPPRPILLGGLVATIQGPMTQLVNVLNAPLRE
                     LVQVLTARSEQAQAAA"
     gene            complement(14628..15350)
                     /gene="rplA"
                     /locus_tag="VFF70_11745"
     CDS             complement(14628..15350)
                     /gene="rplA"
                     /locus_tag="VFF70_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZY45412.1"
                     /translation="MAQHGKKYVEQAKKIDIEKMYAPTEALQLAKETSYAKFNATIEV
                     HFRLGVDPRQADQLVRGVVVLPAGLGKVVRVMVFAQGDGARLAQESGADHVYSTDEEL
                     KKIQDGWTDWDISIAMADMMGKIGRLGKVLGTRGLMPSPKAGTITQNAGDLPRMIKEA
                     KAGRVEFRVDKTSGVHVPIGKASFALNDLVANFEALMDAMKKAKPSGVKGIYIKKVSV
                     AATMGPGVRIDPNLAQSMEAVA"
     gene            complement(15431..15856)
                     /gene="rplK"
                     /locus_tag="VFF70_11750"
     CDS             complement(15431..15856)
                     /gene="rplK"
                     /locus_tag="VFF70_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZY45413.1"
                     /translation="MAKKLKIVVKLNIAAGKANPAPPIGPALAQHGINLMGFCKEYNA
                     RTASMAGQVIPAEISIYTDNSFTFVLKTPPAPDLLRKAAGIEKGSSTPNKQKVAKITR
                     KQLREIAEIKMKDLNSFTIETAMEQIAGTARSMGIDITG"
     gene            complement(16039..16611)
                     /gene="nusG"
                     /locus_tag="VFF70_11755"
     CDS             complement(16039..16611)
                     /gene="nusG"
                     /locus_tag="VFF70_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZY45414.1"
                     /translation="MRAASSEGAPDLSEAPDERAWFVVHCYSGYENKVKHNLEQRIES
                     MGMAHQIFQIVVPTEEEIEVKEGKRRTVEKRVFPGYILVQMIMNDDSWYVVRNTPGVT
                     GFVGMGNKPTPLRTEEVSGILKRMEAEAPKVKVTFKPGQKVRIVDGPFNDFMGTVDDI
                     NMDKAKVRVLVSFFGRETPVELDFLQVEKM"
     gene            complement(16811..17095)
                     /gene="secE"
                     /locus_tag="VFF70_11760"
     CDS             complement(16811..17095)
                     /gene="secE"
                     /locus_tag="VFF70_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZY45415.1"
                     /translation="MADVKQDNRVVHYFKETRAELKKVNWPNRTEAKNLTTVVLVVTV
                     AMAIILFVFDQIASTLLKGVLVDRDTVSIVILLVASVIGLVVLFINLRRQ"
     gene            complement(17194..17266)
                     /locus_tag="VFF70_11765"
     tRNA            complement(17194..17266)
                     /locus_tag="VFF70_11765"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17231..17233),aa:Trp,seq:cca)
     gene            complement(17349..17513)
                     /gene="rpmG"
                     /locus_tag="VFF70_11770"
     CDS             complement(17349..17513)
                     /gene="rpmG"
                     /locus_tag="VFF70_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZY45416.1"
                     /translation="MAKKDVRDVVTLACPECKNRNYTTQKNRRNDPGRVEFNKFCPHC
                     RKHTLHKETK"
BASE COUNT         3911 a         4936 c         4750 g         3955 t
ORIGIN      
        1 ggacggcgcg ctggtgttgc cgctggttgg attgcccggc gtggacgaat cgccgccgat
       61 cttttgcgcg ccatacttcg catcaaactg cgcgccgctc gccaggctga tcgcccattg
      121 accttctgtg gttgattcat agccgaccgg cgctttagct cgcaccgtgt atgaaccagc
      181 cgccagctta ccgaagcaat acggctcact cacaccatcg gtcgagtacg aacctttcac
      241 accgctgtca tcagacaagg tgaagcccac gcctgctaaa gctttttcat cggcatcgcg
      301 cgcggcattg ccgttcgtat catcgaaggc ttgcacacac acctgcccgt tcgattgttg
      361 cactagcggt gccgctaagg cacctccggc caaactgctg aacagcaaca gcatcacgat
      421 cgttattacg atccgccgta gaagctgcat agtctgctcc tttgcaaatg gtaagaatgg
      481 ttaagcgcgg cgtcgagcga taacgaacgc gcctaatacg ccggccacca acaagacgac
      541 taagccaatg ccgcccacca acaagcccga taggctggag cttgatccag tggccggaat
      601 cggcgtcggc gacgcatcgg cgcggctgcc aaagcgaatg tttaccgtcg agccgccggt
      661 caacgacaca ctccagcgct tatcggatgt cgctactgta ttcggcgcgg gttgtaccgc
      721 cactgaataa gtgcctggca ctaagttatc gaagcagtgt ggagccgcgc tgccatccga
      781 tgtataagtg gaaacttgct cgccgctttg tgagttcgct acagtgaact gcacaccggc
      841 caccgattgt tcgccggtgg ttaacaagcc atcggcattc gtatcatcga aagcctgcac
      901 acacaacttc gccgtgatca cttctggggt cgcagttggc gcggccgtcg gcggagcctc
      961 gggggttgtc gaggtgggtg cggcggtggg tagcgctgga ccggtcgggc cacgaaggat
     1021 gatcttttgt ccgatcgaca acaacgcatc tttagtaata ttattcaacg tcagaatttg
     1081 atcgagtggc acatcatacg cgatcgataa ggcgaacagt gtatcacccg attggattgt
     1141 atgcacgatc gagccatctg gattcggcgt gctgatcacc gagggtagcg gcgctggcgg
     1201 cggggcagaa gtcggttgtt gttgtggcgc ggatgtcgca ccgggggcag gcgtcgccgc
     1261 gcccactact gtcaaagcag catcatccca ccaggcatcc agatgtgttc gagtatactg
     1321 acgaaaatcg ttggaagtga acacggtgac ttttccagca ctaccgaccg tcgcctggac
     1381 gcaaagctgc tgccaagtat catgcggatt ggccgcgccg gaccacgtaa cgccggacgc
     1441 ccaattgtcg ccaccattcg gatcgatacc gacgcgcgca cgaccttgaa catctccgtc
     1501 ggaccccgtc cctacggggc tgttgctcgc tcgtattcga gaatatgcgc agaattggac
     1561 cacgctcccg gccggaatat ttgggaccgt ttgcttgata ccagccatcc atggatcttg
     1621 ctggtagccg atgtgttgcg aggtgggatc gccatgaatc aattccggat gagctccaga
     1681 tggattcgac tcggcgccga attgcggcct gatcgtgtag gtcaaggccg atgcatcgcc
     1741 gggcttatca atctgtctat accagcagct ccagccactg gcggtatcac ccgcgccgca
     1801 tccattataa ggctggtcca tgcccgggct ttgcagcaaa ttaccagatt gcgaaaggcg
     1861 ggcggcatgg gcagaattag gcattaggtt gaacagcatc attaacgcca gtgcgaacac
     1921 tccggccagg gccagctcct tgcgaggggt caatttgaac tccatcttct tctccttatt
     1981 ctacatcgcg tctgcttgat gatttgatca tcaacgatct tcttatgata attgcgactg
     2041 aatcagaaat acttgacggg ctgtgattgt ctacgccgcc agataaaaac tgcggtggcc
     2101 gcgatcaata cactcgcccc gaccagcagc tcaggcggaa tgaccacgct ggccggatcg
     2161 atgtgcagcc ccagcgcaat atcaaccgtg gcatcgctat tcaaggccac ggcccaacgc
     2221 cgcgatccgc tgaccgaata accgctcggc gtttgggctg ccacctgata gaccgtggcc
     2281 ggcaaatgat cgaagcaggc ttgatcggca gaaccgtcga gcgtgcgagc aacgatcggg
     2341 ttaccattgc catcgatgac atcgatctga aggcctgcgg cgcttaacgc ttgatcgctg
     2401 atcgatcgaa agccatcacc atcgacatcg ttaaaagcgc gaacgcagat cgtgccgaga
     2461 ccggaacccg ggccgccgat cgatgagatc ggccgtggcg caacgctgat gggcggcagc
     2521 gaaatgacca gctcacgccc gagcgcgagc tgcgattcgg cggacaggcc attcaactgc
     2581 ataatctgat cgaccggcac accgtattgc aacgcgatgg caaataatgt atcgccgctt
     2641 ttgacgatgt gcgtcagctt gcccgtgcgg ccaggcactg cggtcggctg cgcgccaacc
     2701 gccacaacca ccggcggcgg attgacaacg atcggcacgc tcgccccgcc gccgaccagt
     2761 gttagctcgg catcatccgc gtatacgtca ttgtgttcca tcggatattc cggccggctg
     2821 tgtgtgaaca ccgtgacttt gctggatcgc gataccgctt caacttcaaa aagcgtgaac
     2881 tgatcgtacg cttcatgctc agtcgaccag acgatatctt tgctccatgg atcattgccg
     2941 cccgttggat cgatgcccac ccgcaggtgc atactaccct gcccaaaaga gaaatgcgga
     3001 ttcggttcac cttcgctcaa cctcgttgcc gaccagactt ccatgtaaat gctgaatcgg
     3061 agtaaactgc cggggcgaac actggtcacg gtttgataga cacccgcttc gtgcacactc
     3121 caaaacgtaa aatacttttg cgagttctcg ccggatcgaa tacgcggcgg atcttgtggc
     3181 gagctgcgtg tatctctaaa ttccggatta tgccacgcga cacagtcatc cggcgcgttg
     3241 cgcccacagt aattcggata ccggtaataa tccggcgcct ggcgatcggt actgggtatt
     3301 tgccaccagc cgtgccagcc gtctgctact tgaacggtct gccatggcac cgtcggcagc
     3361 gagccatcgc agggcaatgt catccacgga cgtccgccgg gaaaactgca ttgaatctga
     3421 taatcgcctt caaaacccgg attcgtcagt aaattcctgg aactctgatc caatcgcgct
     3481 ggctgcactt gggccgtcgc gatcgtcgat gacatccagg ctaagcacac catcgcaatc
     3541 gcgatcgata tccgctgcgg cttcattcaa ccactcactt taagcgatcg tcttccggcg
     3601 cagtaggacg atccaaccgc cgataccgat cagcgcgccg atgatcaaca acccgatcat
     3661 acccattgcg tccgacgggg ctgccggctt tgattcgctt ggtcggctgc cgaaattcac
     3721 attgaccgtc gcgccgctgc ccagcgtcac gctccaacgc cgatcggatg tagcgatcat
     3781 gccagtgggt gcttgaatcg agactgagta attgcccggc gcgagtttat taaaacaatg
     3841 cggctcgctg atgccgtccg ttgaataggc tgtcagctgc tgtccctgcg catcaaacaa
     3901 cgtgaacagc gcgccagtga ctaacgcttc gctggatatg tagacgccac tggcgtctac
     3961 atcattgaaa gcgcgcacac acaactgcgt cgattccggc gcagccgcca cttgttccgg
     4021 catgggcgtg gccgcgatag tcggcaagct ggtgactgcg ggtgtcaccg tcacgatcga
     4081 ggccgagatg accaactctc gatcgatgac aatgaatgaa tctttggtca aaccgttcaa
     4141 ttgcataatc tgatccatcg gcacattata ctgcaaagac aacccgaaca aagaatcacc
     4201 gggcttgacg gtgtggacga ctgagccatc gggatagggt gtggccgtgg gaccgggcgc
     4261 agtgttgata acagccggtg gcgcggaaat attattcgag acaggcggcg cgccggccgg
     4321 ttgatccggc acttgaatcg ccaccaattc ggcatcgtcc acatacacgt cgttgtgctt
     4381 caagcctttt tccggcatgc tgtgcgtaaa caccgtgacg cgatccgatt gggcagtggc
     4441 acgcacttcg taatagccga agatatcgta gctgtcatgt tcgggcgacc agacaatgtt
     4501 cggactccac ggatcatccc cgccggtcgg atcgatgccc acccgcatgt gcatactcgt
     4561 ctgcccagat gagttgtaag aggagggatc gagttcgctg ccgtcttgat tggacgacca
     4621 cgcgtgcata tacacgccga agcgcagcag actgcccggc agaacattga ccatttggaa
     4681 caggccggct tcatgcacgc tgtaaaacgt gaaatatttt tgcgagtttt tgccggaatg
     4741 aatgcgcgac ggaccggaga ccacgccctt cgtatcgcgg aattcaggat tgtgccaggc
     4801 aatgcagcct tcaggcgcat ccgaataaca attgttggga tgattagagt aaaactttgg
     4861 atccgtgccg tgtttgatat tgggttccaa ccaccagccc gtccaaccga tcggcacctg
     4921 caccgtattc cagatgatat tgatacggcg gcccttctca tctttgacat cgaaatctaa
     4981 gccatcgcac gagatgtgcc gccacggcac atcgctaatg gtgctgcatg ggatatacaa
     5041 cttgccttcg aagccgccat cagtcaacaa attcacgccc acttgcacac tggccggcgt
     5101 gggcgtcaga acaatcggca cgggcgtagc cgtaggatag gatgcttggg ctgtggccgc
     5161 cagatcgcca ctcaacacgc tcaaggcggc atcatcccaa tatacttcat tgttgcgccg
     5221 cgaatcgaag gggttcgcat acgtgaagac cgtaacggtc gtattctgcg atcgggcata
     5281 gaccgagaac tgataccacg aatcgagtat attgcgtggc tgcgtccaga caacattcgg
     5341 gcttttcgga tctgtgccgc cggtcggatc gatgccgatc ttcatctgca tgtccgtgcc
     5401 gcccagcgaa gagttgatgg gatcgttttg ctgcgaggac cagccctgcg catagatcga
     5461 gaattgcaga tcgacattca aaggaacggc cacttgttga taaatgcccg cttcataagc
     5521 gccgaaaaaa tggaacatgc gctgcgcatt gccgccgccg tgaatgcgat gatcgcagcc
     5581 ctgagtgcaa gtgttactgg gactatagtt cggctgcaag aaagcccaat ccggcgcaga
     5641 agctggatgc ggcgtccacc aggcggacca actggtggcc acttgcgcgt ccgcattgat
     5701 ctgcgtaaag cccgtctcaa aatttggatt ctttaagaga ttgggactgg cggcagagat
     5761 cggtgaggcc tgaacgttca gcggcagcca agccacggcg aacgagaata ttaagctaag
     5821 aatcaagaaa cgtctaagtt gcattcaaac accttggtag tctaactgac tgccaagtat
     5881 acatgatttt atttctcaac accctaagtt ttcgcgggtc tacgtaatgt cagatgttta
     5941 cggcccaacc gcatttggac cgcccaacaa aatggcttga tcgatcaccg cctgcgctga
     6001 ctggagtact ttgacgcgct ggccggtcga tcgactatac acgcccattt cgattagcag
     6061 ctgaacccga tcggcttgag ctggcaactc gattgtccgt tgatcgatca cgacatcgcc
     6121 tacccgccac aagctcgccg gataaaagtt gtcgccgcta tcaccatcga ctgaaacaac
     6181 caactgatta ttcaagagca aatgaacaaa gaaggtttga tcattgcttt gcggttgctc
     6241 ggacgtccac aacgttctca accgccaatg cccatcatca attacttcga tcgaatggcc
     6301 gagcaaacgc aagccattat caaacagcgc ttcggtgata ttcgaagtcg attgcttcgg
     6361 atcgatctgc acgcgcaaat aacccacatg cggctgcgga tcgagatcgc cttgagccat
     6421 tggccccgcc gagacttgca gcatgagcgg ccccggcaga ttggcgaatg atttcgaaaa
     6481 atcttcatac ggccagacga atagcgtcgc cgagcgcgcc ggatcgatcg aggcttggcc
     6541 gccgtcggaa agcgtttgat agtcgccgtt cagcaaaaac gaaacgctcg tccaatcacg
     6601 gatgaaccga tcggcgatga agacttgctg gttggatatg atgtcagctt gggcaactct
     6661 ggctaattct gtgcctgcca cctcaaacgc attcgccgtt tcaggatcga ttgcgtattg
     6721 gctataatcg cgcacggtca acacgcccgc catgatgatc gccgcactca tcacgccaat
     6781 gccaatcaac ttcgagcgcg ctgcgatcca tctgccgacg acatccaaac cgatcgccgg
     6841 aatgatcatc actagcggca atatgccaac gacacgcaaa aagtgcggcg ggtcttcggc
     6901 taagatcgtg ggcaacgaca tgactgcgac ccataagagc gtgaaaagtg atgaaagctt
     6961 atttatagcc aagaagaaat tagaatgact atgcgctctc ggcgttcttt gcggtaagac
     7021 tgcacaaact aagcatatca atccgactac aaaaaagatc gccatgatcg gatcgaagac
     7081 cggccgatat ggcacgttgt gacgcgggat gcgatcgccc tgccagaaga acatgcccaa
     7141 tgctttgagt gtgctgctca ctgccgtgcc aacaaaatcg ccgtgattga tgatcgggtt
     7201 aaagatcgat atttcacccg ggcggcccat gactacatcc cactcgttca cggcagtgat
     7261 cgccaacggc gcaatgacca acgcggccgc tgcaaggaag aagataactc gcggccacac
     7321 tcggcgtgaa cgccggacgg cgatcaaaac aatcgagaag acaatcaaaa ccgcgggcgt
     7381 aaatcgactg gcgagatagg tgtaaaatga agcaccatac aataggccgg caatgatcca
     7441 atcgcgccgc cggttcgatc gataggcgcg aatgcccatc gcgatgaaaa gcgcaatgaa
     7501 caatggcaaa ctgacggcgc gcaagccgat gtggctgaga tgaatcggcc agagcgtcac
     7561 tgccatgatc gccgcgctca gaaccccgac acgtcgattg aaaagttcat gcgccagcca
     7621 ataggtggcc ggtacggtta aagtgccgat cagcgccgca ggtaagcgca gagcgatcac
     7681 gctgcgacca aacaagctga cgcttaacga aaccaaataa ataaaaaacg gctcgcgccc
     7741 attattggcc ccgaagtaca acggatgatc gccgcccagc acgcgcagcg catccaagcc
     7801 gttgaaggct tcgtcgtgat acaagccagg cggccaagcg cccagcatat acaaacgtaa
     7861 agccccagcc aagagcatca acccaatgac cggggcccat tcattcaata tgcgtcgcat
     7921 gtgtgagtta acgtgagggc gaactgatca ctccatttgc caaagcggga aaagtataac
     7981 acaataggca cgcgcggcaa agccgcgctg actgcggacg accgaccacc gaccgcagtt
     8041 cgtcaatgac actcattcgc aaccgtgatc cgtggtcctc ggtctacggt cggaacaata
     8101 tgaaagtcgc cattctctct aaagccttaa tcgtctgcgc gtatcgcagc aaactcgagc
     8161 agctggccgc gctgccggat attgaagtga tcgccatcgt gccacccgag tggcgcgatg
     8221 agcgcggcgt tatgcgcatg gaagactcgc ttgataatgt atatgaattg atcgtcacgc
     8281 cgatcgcgtt caacggtaat tttcatctcc atttttatcc gcagatcgat cggttgcttc
     8341 agcgaattcg gcccgacatc ctgcatatcg atgaagagcc ctataatctg gcgacggttc
     8401 tggcactgcg atcggctcag cgcgtgaaag cgcgcacgtt attctttagc tggcaaaacc
     8461 tcaatcgatc ttatccgttt cccttcaatc gcattgaacg ttacgtgttg aaccacgtgg
     8521 atgcctgcat cgcgggcaat caggatgccc aaacggtgtg gcgcgccaaa ggctatcggg
     8581 gcccgatcga gattattccg cagttcggcg tcgatcaaga tatgttcgcg cccgcgccgc
     8641 gcagccgcga cggaacattt gtcatcggct acgtgggccg cttcgttcta gaaaaaggga
     8701 tcgatatttt gttgcaggca ctttcaaagt tgacgggcag ctggcgcgcc gatctattag
     8761 gcagcggtcc ggatcaaacc cgattgatcg atctggccca acgcctgcac atcgaaaatc
     8821 gtgttcgatg cctgccctgg attccatcaa cccgcttgcc ggattactat cattcacttg
     8881 attgcttagt cttgccgtcg cgcacccaat caaactggaa ggaacaattc ggtcgagcat
     8941 tagtggaagc gatgggctgc ggggtgccgg tgatcggatc gaattgcggt gagattccga
     9001 atgtcatcgc cgatgcaggt ctgatctttc ccgaagggaa cgtggatgcc ttgcgccagc
     9061 tgttgatcaa cttgcaagag aatccagcca tgcgcgcgga tctttcgcgg cgcgggcggc
     9121 agcgagtgct ggatcaattt acgcaagcag agattgcgcg caagacgtat ggcgtttatc
     9181 aaaggataaa gcaagctgca tgatgcaaga tgcaaaacgc aaattgcgtt aagcgatgca
     9241 cgacacctga actggtacaa cacagcgcgg ggcaagtgga taccatccgt catttgtcat
     9301 tgttcattgt tcgttgctca ttgctgattg tccatttata atcgccgcat gattgatcaa
     9361 ccgccgcgca ttgcgatcaa cgcgcattta ctcgctccgc gcggcactgg cggctatcgc
     9421 agcgccggaa tccacgttta tacggaacaa gtgctgcgcc atttaccgca agccgacccc
     9481 gatctgcgct ataccgtttt caccgcgcac ccgccgagcg atctcgatcc cgcaattgag
     9541 ttacagacat cgcgctggaa cacggcacgg ccagccacgc gcattgcgtg ggaacagatc
     9601 gttcaaccca gagcaacccg tcgagtcaaa gcggctgtgc tgcacgacac ggctttcgtt
     9661 gggccaatcg tccgaccgtg cccgatcgtg ctgacgattt acgatctgag ttttgcgctg
     9721 tatcctcaat tttttcgcgg tctgaagcaa acgtatttgc gaatcggcac gcgctattcg
     9781 gccaagcgcg ccaagcgcat tatcgccatt tcagacaaca cgcggcgcga catcgcgcga
     9841 ctgtatcagg tgccgctcga tcggatcgat ctggcttatc cgggcgtcga tgaaacgatc
     9901 ggacggcgat cggtcgttga ggtcgaacgc tttcgtagtg aaaagagttt gccgaataag
     9961 tttctgttat ttctgggaac tttggagccg cgcaagaatc tgcccgtgtt gattcgcgcc
    10021 ttcgcgcaga tgaagatcga ttgtcccgat gcaaaattag ttttggctgg cggcttcggt
    10081 tggcggacgg ctgaaatttt taacacggta aaattagcgg gcgtcgaaga tcgagtcctc
    10141 tggccggggt tcgttgcctc tgaagaaaaa acgttgtggt attccgccgc gacggcgttt
    10201 gtgtatccat cgatctacga aggcttcggc ctgccgccgc ttgaagcgat ggcgtgcggc
    10261 acgccaatca ttgtttcgaa tgcagcttcg ttgccggaag tcgtgggggc tgcaggcatc
    10321 tcgatcgatc cgcaagacgt gaatagctgg gccgcggcga tgacccgcgt gtggagggat
    10381 gaagcctatc gggccgagtt agcccatcgg ggtttagccc aagcccaaaa atttagatgg
    10441 atcgaaacgg cacgcgccac gacatcaagt tatcgccgag cgctggaaaa gtaacgtgga
    10501 tcgaagcaat cgcaaacgta tcgtgctgct actgattctg gcgctgcccg cgatcattta
    10561 tatcagcatc gcacaacggc agttcgaaat cacgggcgag aagcgtatcg atggtatcat
    10621 cggtgtgatg ctggggttgt atatttgttc gctggctgcc gccgatctgc tggatatgct
    10681 tttcgcgcgt tggccgcgtc tgtcaaaact aacgcagctg caatggctgg cgttgaatgc
    10741 ggcgttttta gtgttgggca tcgtggtgat tattatgggc acgacgcgct ttacggcgac
    10801 aagctgaact gctcattgtt cattgatcat ggttaatggc ttatgaaacc taagtatccg
    10861 cgctggctca tgtggtgtgt ggtgattggc gacagtgtgc tgatcaacgt ggcgtttctg
    10921 ctggcttaca cgctgcgcta tgacgtgcag ctgctccgcc cggtagaacc gatctttgat
    10981 aatccaccga cggtctacat tccatttgcc gttttgctga ccggcctgct gctgatcaca
    11041 ttcaagatcg acggcgtcta tcaatcgcgc cgactgggta tgtggctgga gcagatgact
    11101 gcgattgtgc gcgggacgac gttaggcatt ctactgatgg tcggcatcac gttcttttat
    11161 cagccgactt tttattcccg attgatcttc gtgtacgaca gcgtgttgat cgtcttgctg
    11221 ttgggcgtcg gccgattggt ctggggcttt attctcgctc agttacgacg gcgcggcatc
    11281 ggcgtggtgc gtgtgctgat cgtcggcgca gacgaagtcg gccgcacggt gattcgcacc
    11341 gtgatggctc agccggagct gggctatcgg attgtgggcc taatcagcga tcaattcgat
    11401 acgaatacgt tgaacatcgg gccgattccg ctgttgggcg gcctggaaaa aataccgcaa
    11461 gcgatcagcg agcaacgcat cgacgaagtg gtgattgcgc tgccgtggac tgatcagcag
    11521 cgcgtgctgg aagtgtttca aacgtgtgat cgcttgggtg tgcgcgcccg caccgtgccg
    11581 gatatttttc aattgagttt aaaccgcgtc gatgtggaag acttgggcgg cgtgccgttg
    11641 ttgggcttga agtcggcctc gtttcgcggt acgaatctgc tggtgaaacg cttgatcgat
    11701 ttgatcgtcg gtggcctgat ctctttggtc gcactgccgt tcatgctgct tctgtcaatc
    11761 gccattcgga tcaattcgcc cggggcgatc atcttcagac aaaaacgcgt gggcatgcgc
    11821 ggcaaagaat tcacgattta caaattccgc accatgcgcg cgggcgcgga agaagaacag
    11881 gaacgcctgc tggtctacaa tgaatttacc ggcccgctct tcaagatgaa aggcgatccg
    11941 cgcatcacgc aagtgggacg cttcctgcgc aagaccagcc tggacgaact gccacaattg
    12001 ttcaacgtca tgaaaggcga catgagtctg gtcgggccgc ggccgcacat cgcgagtgaa
    12061 gtggcacaat atcaggactg gcaaaagcaa gtgctggaag cgccgccggg catgaccggg
    12121 ctgtcgcaag tgtcaggccg cagccagctg agtttcgacg aacaatgttt gctcgacatt
    12181 tacttcatcg agaattggtc gcccgcatta gactttaaga ttttgctgcg caccattccg
    12241 aaagtcgtac tgggcgaagg cgcatattga aaaggatgaa agacgaatta gacgagtggg
    12301 cgatatcgcg cgcattgggt cgcatggcga agtatttgcc ggatcgatcg aaagacacag
    12361 ccttagcgct ggctcgcaac tcgatcgatc cttcggcgcg aatacgagcg ttgagcggct
    12421 tgatcaatgt tctgcctccg atagaccacg cagccgcact acaagaagcg caaaccgctc
    12481 tacacagttt atcgaacccg tggctgcgcg tgcgatcaat gttgatcttg gcgctgcgtc
    12541 tctcacccga agcgaaagat cgaattctcc gtgaagcatt cggcaccacg ctgaatattc
    12601 ccaacgattg ggtgcgcggc cgcgcgctca atttgatcta cgggcattta cccgccgact
    12661 tgatcgatcg cgcgatcgag gcggcaagca agttgcagaa tctggatatg cgcaccgtgg
    12721 cattgagctc aatggctgcg cgattgagcg atccattaac aagcgaagtc ctgcgtgata
    12781 ttccttcgat cgaggacgag agtctgcgcg cgtcggcgct aaacgatctg gcgcggcgct
    12841 taacgcccga tcaattaaag catgctttat cgatcgcata cgggcttcaa gacgaaacgg
    12901 cgcgggttga tgcgttgagc ggcttgagcc agcagctcgc tgagccgatc aagaacaagg
    12961 tgatcagtga gatgcttgac atcactcagc acgccgatga cggcactcgc cgcgcggaaa
    13021 tgctgctact gatcgtcact cgccagccgc aagtgctgtc gtcgagcatc gtagctgaaa
    13081 taacccgcgc gatacaaaaa acgatcgggg ccgacatccg cgtggactta ttcacgaaac
    13141 tggccggtct attgccgcca gagcagcgat cgatcgtgtt aacagaggcg ttgagcgccg
    13201 cccgatcgat cgagaatgct gcagcacgcg ctgaagcatt gagcggcttg atcggcttta
    13261 tatcgatcga tcagcgcgaa gccatcgttc atgaagtttt ggcaatgctt caggtttaac
    13321 aatgggtatc gaccaaatca aaaagcctct tcatgatgaa gaggcttttt gattgaaaga
    13381 ctgatcttac ttgacttcga cggatgcgcc ggcagcttcg agcttggccc tggcatcggc
    13441 ggcggcttct ttgctgaccg cggtcaacac cgcatgcggc gctgtatcga ccaattcctt
    13501 ggcttccttc aggcccaagg tgttgacgag ctggcgcacg actttgatcg tatcgatctt
    13561 cttcgcgccg ccatccttga gaatcacatc gaattcagtc tgctcttcga ccggggccgc
    13621 ggctgccgcg ccaccaccgg cgggcatggc gcccatcatc atcggagcgg cggcactgac
    13681 gccccacttc tcttgcagct gtttcgacag ttcagccgct tccattaacg tcaacgcatc
    13741 caactgcgcg acgatttgat ccaatttttc agacatcgtg atattcctcc atggaatttc
    13801 taattgattg attctttgat tttcaaagag cgatcgctga acgcctcgct catacctgca
    13861 ctcaagccgc agcctgagct tgctccgatc gcgcagtcag cacttgcacg agctcgcgca
    13921 aaggcgcgtt caacacgttg accaactggg tcatagggcc ttggatcgtc gcgaccaagc
    13981 cgcccaacag aatcgggcgt ggcggcagat cggccagggc tttcacgccg gcaacatcga
    14041 tcaaccgctt gtccaacaga ccgcctttca ctttaaagga ttgcttgtcg ttggcgaagt
    14101 tgaccatcgc ttttgcgata actgccgggt cctggaagca gaaacccatg atgacggaac
    14161 cggccaataa ctcttccggc accggcaggt tagctgcttt gagcgcgcgc gccgccaaag
    14221 tattcttgac gacatgcagc tcgccgttcg cttcgtggat cttcaatcgc agctggtaca
    14281 aatccttggt caacacaccc tgataactgg cgatgatcac cgcctgactg tgcttcagcc
    14341 attcggtgta ttcgttgatc aattcgctgc gacgttgttt agatacgggc acttgtctca
    14401 cctccttccg tctgattgct cctgaacttt ggaacaaaaa agtctttgcc gcaaaaccaa
    14461 ggcaaagact cagacgctgc gggacacagt acccgttcag atcatcttgt ggctttcacc
    14521 tcggcaggtt cggattaagt cgatcgagtt gatcgacgcc tgcggtcatt ggctgcggca
    14581 agtattcagt ttttggaacc tccccaaaac cctgtgggag gttttaacta agctacagct
    14641 tccatcgatt gagccagatt cggatcgata cgcacgccgg ggcccattgt ggctgcgacg
    14701 gaaactttct tgatgtaaat accttttacg ccgctgggtt tggctttctt catcgcatcc
    14761 atcaacgcct cgaagttagc aacgagatcg ttgagcgcga atgacgcctt gccgatcggc
    14821 acgtgcacgc ccgacgtttt atcgacacgg aattctacgc ggcccgcttt cgcttctttg
    14881 atcatgcgcg gcagatcgcc ggcattctgc gtgatcgttc cggccttggg gctgggcatt
    14941 aagccgcgcg tgccgagcac tttacccaag cggccaatct tacccatcat atcagccatc
    15001 gcgatcgaaa tgtcccaatc ggtccagccg tcttgaattt tcttcaattc ttcatcggtg
    15061 ctgtacacgt gatcagcgcc ggattcctgc gccaagcgcg cgccatcgcc ttgcgcgaag
    15121 accatcacgc gcacgacctt gccgagtccg gccggcagca cgacgacacc gcgcacgagt
    15181 tgatcggcct gtcggggatc gacgcccaat cggaagtgca cttcgatcgt cgcattgaac
    15241 ttggcgtacg atgtttcttt cgccagctgc aaagcttcgg tcggcgcata cattttttcg
    15301 atatcgattt ttttcgcttg ttcgacgtac ttcttaccgt gctgagccat gttattctcc
    15361 tggtggtgta aatggtagca cgcctaggcg agactaccat agcggatcgc tcgatcctcc
    15421 cactgaatga ttagcctgta atatcgatgc ccatgctgcg ggccgtgccg gcgatctgtt
    15481 ccatcgcggt ctcgatcgtg aacgagttca aatctttcat cttgatctcg gcgatctcgc
    15541 gcagctgctt gcgagtgatc ttcgccacct tctgcttgtt cggcgtcgac gatccttttt
    15601 cgatcccggc ggccttccgc agtaaatcgg gcgcgggcgg cgtcttcagc acaaatgtga
    15661 atgagttgtc cgtgtagatg ctgatttccg caggaatcac ttgcccggcc atgctggccg
    15721 tgcgcgcgtt gtattccttg cagaagccca tcaagttgat gccgtgctgt gccagcgcag
    15781 ggccgatggg cggcgccgga ttggctttgc ctgcagcgat gttcagttta acaacgatct
    15841 tcagtttctt agccatgctt ttctcctggt ggtgtaaatg gtagtacgcc taggcgagac
    15901 taccatagcg gatcgatcga tcctcccaca aatttttgtc actgcacgcg tcattgcgac
    15961 cctgtacaag gcagataggg caggttgagc atcttttcgc gacgaagcaa tctcagtatc
    16021 gacattggga ttgtcaacct acattttttc gacttgcaag aaatccaact cgacgggcgt
    16081 ctcgcgcccg aagaacgaca ccaagacccg caccttcgct ttgtccatat taatatcatc
    16141 gaccgtgccc atgaaatcgt tgaacggccc atcgacgatg cgcacttttt ggccgggctt
    16201 gaaagtgact ttgactttcg gcgcttcggc ttccatgcgc ttgagaatcc cggacacttc
    16261 ttccgtgcgc agcggagtgg gcttgttgcc catgccgacg aatccggtga cgcccggcgt
    16321 gttgcgtacc acgtaccaag aatcatcgtt catgatcatc tgcaccagaa tatagccggg
    16381 gaacacgcgc ttctcaaccg tgcggcgctt gccttcttta acttcgatct cttcttcggt
    16441 cggcaccacg atctggaaaa tctggtgagc catacccatc gattcgatgc gctgctccag
    16501 attgtgtttc actttgtttt cgtaaccgga atagcagtgc actacgaacc acgcgcgctc
    16561 atcgggcgct tcgctgagat cgggcgcgcc ttcagacgag gcagcccgca tggccgaggt
    16621 gcgcagctcg cgcagtgtcg ctttgacatc ctcggcatcg gacggtagtt cgatcaccga
    16681 ctcttcgatg ggttcatccg ctggaagctg atcttcgatc ggagctaacc cgatcgcctc
    16741 tgcgggctga tcggactcaa tcggcatcat ttcttctggc agggttgctt catccatcat
    16801 acaaactcac ttattggcgg cgcaggttaa tgaacaaaac aaccaggcca atcactgatg
    16861 cgaccagcag aataacaatg cttaccgtat cgcgatcgac cagcacacct ttcaataagg
    16921 tggaggcaat ctgatcgaat acgaacagaa taatagccat ggccaccgtc acaaccaaaa
    16981 ccactgtcgt caggttcttc gcctcggtgc gattgggcca attgactttc ttcaattcgg
    17041 cgcgtgtttc tttgaagtaa tgaactacgc gattatcttg tttgacgtcc gccacgatgg
    17101 agtgactcct cttaactcgt caggctgcgc cggtgcgctt tcaaaattat tggccgcgcc
    17161 cgatcgatct cgcgcggcca aacggttctt aggcaggcga ggcaggaatt gaacccgcaa
    17221 cccccgcttt tggagagcgg tgctctgcca attgagctac tcgcctgtaa gcatattgtt
    17281 taagcatagc gcacgatcac aattctggca acgatcaagc gctaggcaat aagcgatact
    17341 ccgatctgct acttcgtttc cttgtggagc gtgtgcttgc ggcaatgcgg gcagaacttg
    17401 ttgaattcca cgcggcccgg atcattgcgc cgattcttct gcgttgtata gtttcgattc
    17461 ttgcattcgg gacacgccag tgtcacgaca tcacgcacat ctttcttagc cattgttctc
    17521 tctcttttcc aactgtacga aagctctaaa cc
//
LOCUS       DASRTJ010000577         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_263692,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000577 DASRTJ010000000
VERSION     DASRTJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263692"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFF70_11775"
     CDS             complement(<1..341)
                     /locus_tag="VFF70_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45417.1"
                     /translation="MTSPSSKSNSSISFIEWLLIVLIVAVVLLLSGILGLGNLPAADS
                     IVGISTRTATATATLTFTPTPTFIPTSTPTATPTNTPFPTAPPRPSGAILPLIIVTAG
                     EVISGTPVPTQ"
     gene            complement(575..>1808)
                     /locus_tag="VFF70_11780"
     CDS             complement(575..>1808)
                     /locus_tag="VFF70_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZY45418.1"
                     /translation="AFTDIYTDTPVPTPVPLIDLPPETINIALLGSDARPNTGGWRTD
                     VIIIVSINPAVPSVAMFSIPRDTWVYIPNWQYTRVNLADEHGEFVQFPGGGPGLLEQT
                     LQYNFGIPFQYYARVDFEGYKKLIDSVGGVDVVADCPLFDIFPDNPPGVTDIGFSDIT
                     GTIDIPTPGIYHLDGKHALWYSRSRKTTSDFDRSRRQQHVLNALWDKIREQGLINKLP
                     DIWSDVVSSVQTALSLNDVIYLAGLGQTIDRSRIRSSFLDGAYAHRFTSVEGASVFSY
                     SYDEISQTVQTAFWPPLIGRAVQPPATIEVFNGTSNPDWDAIAADRLSSAGYHIVSYG
                     PADRADYPATAITDLRATSKGSRLAELSRLFNTRNAAYQSDPNTPAEYRIILGADFQP
                     CQRARIGTFPTATPTPPP"
BASE COUNT          436 a          436 c          540 g          396 t
ORIGIN      
        1 atctgcgtcg ggacgggtgt gccgctgatt acttcaccgg ctgtgacaat gatcagcgga
       61 aggattgcgc ccgagggacg cggcggcgcg gtcgggaatg gcgtgttcgt cggtgttgca
      121 gtcggcgtag aggttggaat aaaggtcgga gttggcgtaa atgtcagggt tgcagtcgca
      181 gtcgccgtgc gcgtcgagat acccacaatc gaatcagccg ctggcagatt gcctaaaccc
      241 aaaataccgc tcaagagcag cacgacggcg acgatgagga caatgagcag ccactcaata
      301 aaactgatgc tcgaattgct tttacttgac gggctcgtca tgatctatta cacatctcgt
      361 aaaatattgg ctgagcatct tcatcaaagg tgcgagccgt gtgattatac aggacgagca
      421 ggcaatgctc aatacaaaat gaataagtga ttgcagacca tgggcttcag actgcagagg
      481 atgcgttggc tatcttcagc ccgcgtcgtt cagaaaaaac tcggtgatcg aaaatgatca
      541 actgccgtct tcggtctgtg gtcttcggtc gtctttacgg agggggagtc ggtgttgcag
      601 ttggaaaagt gccgatgcgg gcacgctggc acggttgaaa atctgcgccg aggatgatcc
      661 gatattctgc gggagtattc ggatcagatt gataggctgc attgcgggta ttgaagaggc
      721 gtgacaattc tgctaagcgc gatccttttg atgtcgcacg caaatccgtg atcgctgtcg
      781 ccggataatc ggcgcgatcg gccggaccat aactgacgat gtgataaccg gcggaactca
      841 atcgatcagc ggcgatggcg tcccaatctg gattcgatgt gccattgaac acttcgatcg
      901 tcgcgggtgg ttgaacggcc cggccgatca gcggcggcca gaaagcagtt tgaacggttt
      961 gggatatttc gtcatacgag tacgagaaca cgctggctcc ttcgaccgaa gtgaagcgat
     1021 gcgcgtacgc gccgtcgaga aaactgctgc gaatgcgcga tcgatcgatc gtttgaccga
     1081 ggcccgctaa atagatcaca tcgttcagac tcagagcggt ctgcacgctg ctgacgacat
     1141 cgctccaaat atcgggcaac ttattgatca agccttgctc gcgaatcttg tcccacaacg
     1201 cattcaacac atgctgctga cgacgcgatc gatcaaagtc gctggttgtc ttgcgcgaac
     1261 gcgaatacca caacgcatgt ttgccatcaa gatgatagat gccgggcgtt ggaatatcga
     1321 tcgtgccggt gatgtccgag aagccgatat cggtaacgcc gggcggatta tcgggaaaga
     1381 tatcgaataa ggggcaatcg gccaccacat cgacaccgcc gacagaatca atcaattttt
     1441 tgtagccttc aaaatcgacg cgtgcataat actggaacgg gattccgaaa ttgtattgca
     1501 gggtttgttc caagagaccg gggccgccgc cgggaaattg aacgaactcg ccatgctcgt
     1561 cggcaagatt gacacgggta tactgccagt tgggaatgta gacccatgta tcgcgtggaa
     1621 tgctgaacat cgccacggag ggcacagcgg gattgatgct gacgatgata atcacatcgg
     1681 tgcgccagcc gccggtgttg ggacgcgcgt cgctgccaag cagtgcaata ttgatcgttt
     1741 cgggtggcag atcgattaac ggtacgggcg tgggcacagg tgtgtcggta taaatatccg
     1801 tgaaggcc
//
LOCUS       DASRTJ010000578         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_36161,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000578 DASRTJ010000000
VERSION     DASRTJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36161"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFF70_11785"
     CDS             complement(<1..607)
                     /locus_tag="VFF70_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY45419.1"
                     /translation="MYLRHLSLTNFRTYARLEIDLPAQAILLYGDNAQGKTSLLEAVY
                     VLATARSPHTVSDRQMIHWLAGEETKSPYARLVADVIKSDRTQHIEMVLMLEPANTED
                     GWRFRKQLKLNGSVVRAADLVGQIAVVMFLPDDVDIVAGSPGSRRRYLDTAVSQVDAE
                     YSHALDLYADVLSQRNALLKQLSESGGDPDQLIYWDEQLAVS"
     gene            905..1486
                     /locus_tag="VFF70_11790"
     CDS             905..1486
                     /locus_tag="VFF70_11790"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZY45420.1"
                     /translation="MTHDSSGRECILDTSAKLFNEKGFKGVSIRDIAQACGMTNAALY
                     YHFKNKDDLFLEVLQQNHARATAVLDEAARSGGSLRSRLKQLTHSYFEVMLAQRQSMH
                     MLWRDLKHLDDARASKLYTDMRTGFMQPLQQLIESAQSAGEIVGGDAQLYARLLHGML
                     IALTFEGRKNSKAQVSANEVDALVKVFLDGVGK"
     gene            1537..3507
                     /locus_tag="VFF70_11795"
     CDS             1537..3507
                     /locus_tag="VFF70_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45421.1"
                     /translation="MKRQAEQGQSKRPAGPMGKRGAGQWGGGQGRPKVDFGALRRALG
                     YVGHYRRIAIIAYGSLFVATAAQLAVPTLVRSIIDSVTNGYIATNVLKAPASILPAIE
                     QKLGLTHEQLVANAANAESMLIAAGIAIVVFALVRGIFSFLQSYNAERLSQSAAFDMR
                     NDLFSKIQRLSFSYHDQNQTGQLMVRATDDVEKVRLFIGQGLLQALQALILLAGTLLV
                     LVLTNVQLTLAVLPILPLALILFVIFGRISQPLFVVVQIKLSKLNTILQENLAGIKVV
                     KAFAREPQEQKKFNLSADDLMNQQIRVTRIFAFLFPLIFMIANLGQASTLYFGGRLII
                     GGTLTIGEWQQFSLYLAYVFFPIGQLGFIITQMSQAAASASRVYEIIDAKSDVIDKPD
                     AIALPSINGRVEFKNVTFRYPGGQAVLTQVNLEAVPGQTIALLGATGSGKTSIINLLP
                     RFYDPSEGQVLIDGHDLRDITIDSLRQQIGIVLQETNLFSGTIKDNIAFGRSSASLDE
                     VIAAAKAAAAHDFILEFPDGYDTPVGERGSTLSGGQKQRVAIARALLLDPRILILDDS
                     TSSVDLVTEYKIQQALDRLMKGRTSFVIAQRISTVLNADQIIVLDRGSIVARGTHEEL
                     IETSEIYAQIYSSQLLEDVHIDEPATESVKAA"
     gene            3504..>3979
                     /locus_tag="VFF70_11800"
     CDS             3504..>3979
                     /locus_tag="VFF70_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZY45422.1"
                     /translation="MKIAIIGGAGRSGQLLIDRALASGYEVTTFVRDRSKIAIQHDRL
                     KIVEGDVRDLDRVNAAIQNQAAVISVLGQTRPPTKELLTIGVGNILKAMQQNNVTRLV
                     YLTGAGVRSEKDSAPPIAAKVMMFLLKKMAADVLQDSEQAAELIKRSDREWIIVRVP"
BASE COUNT          918 a         1022 c         1142 g          897 t
ORIGIN      
        1 cggacacggc taactgctcg tcccagtaaa tcagctgatc gggatcaccg ccactctctg
       61 atagctgctt caataatgca ttgcgctgcg acaaaacatc ggcatataga tcgagcgcgt
      121 gtgagtattc ggcatcgact tgcgacacgg cagtgtcgag gtagcggcgt cggctgcccg
      181 gcgatccggc gacgatgtcc acatcatcgg gcagaaacat cacgaccgcg atttgcccca
      241 ccagatcagc cgccctcaca accgatccat tgagtttgag ctgcttgcga aagcgccagc
      301 catcttcggt gttagccggt tcaagcatca acaccatttc gatgtgctgc gttcgatccg
      361 atttgatcac gtcggcgacc aaacgcgcgt aaggcgattt tgtctcttcg cccgccagcc
      421 agtggatcat ctgtcgatcg ctaacggtgt gtggcgatcg ggcggtggct aaaacgtaaa
      481 cggcctcaag caaactggtt ttgccttgcg cgttgtcgcc atagagcaaa atcgcctgcg
      541 caggcagatc gatctccaga cgggcgtagg tgcggaagtt ggtgagtgag aggtgacgga
      601 ggtacatgat gagtgatgag caacgagtga cgagtaatga gtaattttat tgtaacacag
      661 agcgtctttt caacagcatg tcagcttgcc ccttgcaacc tgtaactttc aacagttgac
      721 agctggcggg cgctatgata aactctgcgg gctttactga acgtttggta agtacctgcg
      781 ccgtcattgc gagcgaagcg aagcaatctc aatttcgatc aggagattgc ttcgtcggcc
      841 taacggcctc ctcgcaatga cgcctgaact gcgtaaggtg cgtaagtaag ttggaggcag
      901 ctttatgact cacgatagca gcggccgcga gtgcattctt gatacctcgg ccaaattatt
      961 caacgagaaa ggctttaagg gcgtgtcgat tcgcgacatc gcgcaggcct gcggtatgac
     1021 caatgcggca ctatactatc acttcaaaaa caaagacgat ttgttcctgg aggtgctgca
     1081 gcagaatcac gcgcgcgcta cggccgtgct ggacgaagcg gctcgatcgg gcggcagctt
     1141 acgatcgaga ctgaagcagc tgacccatag ctactttgaa gtgatgctgg cgcagcgcca
     1201 atcgatgcat atgctatggc gcgatctaaa gcacctcgat gatgcccgcg ccagtaaatt
     1261 gtataccgat atgcgcactg gcttcatgca gccgcttcag cagttgatcg aatcggcaca
     1321 gtccgccggc gaaattgtcg gcggcgacgc gcagctgtat gcgcgtttgc tgcacggcat
     1381 gttgattgcg ttaacctttg aagggcgaaa aaacagcaag gcgcaggtga gcgcgaatga
     1441 agtcgatgcg ctggtcaaag tatttttgga cggcgtcgga aaataatcgt acagccgatc
     1501 cggtggatcg gctcaattac ttgaagggga aatgatatga aacgtcaagc ggaacaagga
     1561 caatcgaaga gacccgccgg gccgatgggc aagcgcggcg ccggacaatg gggcggcggt
     1621 caaggtcgac ccaaagttga tttcggtgcg ctgcgacgcg cgctgggcta cgtcgggcat
     1681 tatcgccgca ttgcgatcat cgcctatgga tcgttattcg tggcgacggc cgcccagctc
     1741 gccgtgccga cgttggtgcg cagtatcatt gattcggtga ccaacggtta catcgcgacg
     1801 aatgtgttaa aagcaccggc gtcgattctg ccggcgatcg agcagaagct gggcttaacg
     1861 cacgagcaat tagtggcgaa tgccgccaac gccgaatcga tgttgattgc ggccggtatt
     1921 gccatcgtcg tattcgcttt ggtgcgcggc attttctcat ttctgcaatc gtacaacgcc
     1981 gaacgcttgt cgcaaagcgc agccttcgat atgcgcaacg atctgttctc caagattcaa
     2041 cgcctctcct ttagttatca cgatcaaaat caaaccggcc agttgatggt gcgcgccacc
     2101 gatgatgtgg agaaagtccg tttgttcatc gggcaaggtt tattgcaagc gctccaggct
     2161 ctcatcttgt tggcgggcac attgctggtg ttggtgctga cgaacgtgca gttgacgctg
     2221 gccgtgctgc cgattctgcc gctggccctg atcttatttg tcatcttcgg tcggatcagt
     2281 cagcccttgt ttgtcgttgt gcagatcaaa ctctccaagc taaatacgat cttgcaagaa
     2341 aacctggcgg gcatcaaagt agtcaaagcc tttgcccgcg aaccgcagga gcagaagaag
     2401 ttcaatctgt cggccgatga tctcatgaac caacagatca gagtcacgcg catcttcgcg
     2461 tttttgtttc cgttgatttt catgatcgcc aacctgggcc aggcttccac cttgtatttt
     2521 ggcggtcgat tgatcatagg cggcacattg acgattggcg aatggcagca gtttagtttg
     2581 tacctggctt acgtcttttt cccgatcggc cagctgggct ttattattac gcagatgtcg
     2641 caagcggcgg catcggccag ccgcgtttat gaaatcatcg atgctaaaag cgatgtcatc
     2701 gataagccgg acgcgatcgc gctgccttcg atcaacggtc gcgtcgaatt caagaatgtc
     2761 acgttccgtt atcccggcgg ccaagcggtg ttgacgcagg tgaaccttga agcagtgccc
     2821 ggccagacga tcgcgctgtt gggcgcgacc ggcagcggca agacatcgat catcaatttg
     2881 ctgccgcgct tctatgatcc gagcgaaggc caagtgttga tcgacggcca cgatttgcgc
     2941 gacatcacga tcgattcgct gcgacagcag attggcattg tgctgcaaga aacgaatcta
     3001 ttcagcggca caatcaaaga caacatcgcc ttcggtcgat cgagcgcgtc gctggacgaa
     3061 gtgatcgcgg cggcgaaggc cgcagccgcg catgacttta tcttggaatt tcccgatggc
     3121 tacgatacgc ctgtcggcga gcgcggttcc acgttgagcg gtggacaaaa gcagcgcgtg
     3181 gccattgcgc gcgccttgtt actcgatcca cgcattttga ttttggacga ttcgaccagc
     3241 agcgtcgatc tggtgaccga atacaaaatt caacaggcgc tcgatcgatt gatgaaaggc
     3301 cgcacaagtt ttgtcattgc acagcgcatc agcacggtac tgaacgccga tcagattatc
     3361 gtgctcgatc gaggctcgat cgtcgcgcgc ggcacgcacg aagaattgat cgagaccagc
     3421 gagatttacg cacagattta cagttcgcaa ttgcttgaag atgtgcatat tgatgaacct
     3481 gcaactgaat cggtaaaagc cgcatgaaga tcgcgatcat cggtggggca ggccgatcgg
     3541 gtcaattgct gatcgatcga gcgttggcga gcggctatga agttacgact tttgtgcgcg
     3601 atcgatcgaa gattgcgatc caacacgatc gattgaagat cgttgaagga gatgtgcgtg
     3661 atctcgatcg agtgaacgcg gcgatccaga atcaagcagc ggtgatcagt gtgctgggtc
     3721 agacgcggcc gccgacgaaa gagttgttga cgatcggcgt cggtaacatc ttaaaagcga
     3781 tgcagcaaaa taatgttacc cgcttagtct atttaaccgg cgcaggcgtg cgcagcgaaa
     3841 aagacagcgc gccgccgata gccgcgaaag tgatgatgtt cttgttgaag aaaatggcgg
     3901 ctgatgtgct gcaagattcg gaacaagccg ccgagttgat caaacgcagc gatcgagagt
     3961 ggatcatcgt tcgggttcc
//
LOCUS       DASRTJ010000579         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_356096,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000579 DASRTJ010000000
VERSION     DASRTJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356096"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..658)
                     /locus_tag="VFF70_11805"
     CDS             complement(392..658)
                     /locus_tag="VFF70_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45423.1"
                     /translation="MRDASTACDASGVRESSITCWWRVDRVRGVCATARMPRTRNMLT
                     GDPHRVLNQIGVPIAKVNTVNDVLNEPLFYNDFAHAKDPRTLIE"
     gene            complement(677..1213)
                     /locus_tag="VFF70_11810"
     CDS             complement(677..1213)
                     /locus_tag="VFF70_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45424.1"
                     /translation="MSNNSLYRTAGWCALLTALLTVVVFVIFTVAPTSSLTLILGVVS
                     LLVMTLVFYALYVAHRSESAGLSLAGLILWIVAGVANIASLANPTNNALYAVSTMVFA
                     VPILIFGFLAYRSPKMPRGLAVVALLTGVVWGLAGAVSFNGPTDIAMAANLAATVFWL
                     VWLVWLWRVFSSGKLAAA"
     gene            complement(1472..>2014)
                     /locus_tag="VFF70_11815"
     CDS             complement(1472..>2014)
                     /locus_tag="VFF70_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZY45425.1"
                     /translation="MLTAMGEQVTRRGNTHQFFAPVSVYPTRDGFVYIAVGNDRQWAA
                     LTKLAGFESLIDDQYQRNAGRIADVKQLNERISDCTRNFATDDLIPGLNQIGVPIAKV
                     NTLNDVLNEPHLRKDFAHAKDPRTSIEVTLSPIAEVNADRVSELSFPPRLGEHNASIY
                     GEVLGYSTDQLAELRNRQVI"
BASE COUNT          488 a          573 c          488 g          465 t
ORIGIN      
        1 atgtggatca gatgcaggat gatcgaataa atgtccattg ctctactcct attaaagaat
       61 agttcgattt gcactcaagt cgcacgagtg tttccctcat tcaaatcacc tgccgaccgt
      121 gtcggggcga ggcagtcgca caacaatcct ggtcgatcga catcgcggcc acgtccaaaa
      181 acccgatcga catgaattca ttggcgactg cctcgccccg acgatcacga tcaatcactt
      241 caaatcactc cacgttcttt caactcgatt aatttatttc gcgaataacc taaggcttcg
      301 ccgtagatcg attcattgtg ctcgcccaat cgcggtggga acgaaagttc ggacactcga
      361 tcagcgctca cttcggcaat agggcttaag gctattcgat caacgtgcgc ggatctttcg
      421 cgtgcgcaaa atcattgtaa aatagcggtt cattcaagac atcattcacc gtattcactt
      481 tggcaatcgg cacgccgatt tgattcagca cccgatgagg atcgccggtc aacatgttgc
      541 gggtgcgcgg catgcgtgcc gtagcgcaaa cgccgcgcac ccgatccact cgccaccagc
      601 acgtgatgct tgattcacgc acaccacttg cgtcacacgc agtgctggcg tcacgcatca
      661 cgttgcctcc cttgtgctat gccgccgcaa gttttcccga cgaaaagaca cgccacaacc
      721 acaccaacca taccagccaa aagacggtcg cagccaggtt agccgccatt gcaatatcgg
      781 tcggcccatt gaatgagacg gctccggcaa gcccccagac aactccggtc agcaaggcca
      841 ccactgcaag cccgcggggc atcttgggac tgcgataggc aagaaaacca aagatcagaa
      901 ttggcactgc aaacaccata gtggagacgg cataaagagc attgttagtc gggtttgcca
      961 agctggcaat gttcgccaca ccagcaacaa tccacaagat caggccggcg aggctcagcc
     1021 cagctgactc tgagcggtgg gcgacgtaga gcgcatagaa aacgagagtc atcaccaaca
     1081 aacttaccac gccgaggatc aaggtcaggc tgcttgtagg cgctaccgtg aaaatcacga
     1141 agaccaccac cgtgagcaga gcagtcagga gagcgcacca tccggcagta cgatacaggc
     1201 tattgtttga catttgtttt tctccttatt ttttagtatt ttaatttcac acagatggat
     1261 gatcgaacgg gtcgttttct tttgcttgct ccatcacctc cttcaagaac tcgagcagct
     1321 ggtccaagtt cgcaaacccg cgccactcga ccgtgttttg ggactttgca ttttctggct
     1381 gaatatcacg cgaccatctg cgagcacttc agcgaacgat tgaacagtct gtctctttct
     1441 gaatagaaaa ttcattgagt gtttccttca ttcaaatcac ctgccgattg cgaagttcgg
     1501 ctaattgatc agtgctatag cccagcacct cgccgtagat cgacgcgttg tgttcgccca
     1561 atcgcggcgg gaacgaaagt tcagacactc gatcagcatt cacttcggcg atcgggctta
     1621 acgtcacttc gatcgaagtg cgcgggtctt tcgcatgtgc aaaatctttg cgaaggtgcg
     1681 gttcattcaa gacatcgttc agcgtgttca ctttcgcaat cggcacgccg atttgattca
     1741 gcccggggat cagatcgtcc gtcgcgaagt tgcgtgtgca gtcgctgatg cgttcattca
     1801 actgcttcac atcggcgatg cggcccgcgt tgcgttgata ctgatcgtcg attaatgact
     1861 cgaagcccgc taacttcgtc aaggctgccc actgtcgatc attgccgacg gcgatgtata
     1921 caaagccatc gcgcgttgga tacaccgaga cgggcgcaaa gaattgatgc gtgtttccac
     1981 gccgtgtcac ttgctcgccc atagcagtca gcat
//
LOCUS       DASRTJ010000580         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_280627,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000580 DASRTJ010000000
VERSION     DASRTJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280627"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFF70_11820"
     CDS             <1..1186
                     /locus_tag="VFF70_11820"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZY45426.1"
                     /translation="IIGTLATRTGLLSSVHAFARSSLSVPFLAFVGFVLVGSTVLMII
                     RSDVLKSDNKLDSIFSREAAFVLNNFLFVTITFTVFIGTWYSLISEALIGEKLTVGPA
                     VYNQMLGPQVALLILLMGIGPLVAWRRSSAEALGRMSWKSALLAMAVMIALYLSGIQA
                     IWVLVGFGLIGYTFGVTISEYWRGARARHKLSGENYFIALVRLFQRNRRRYGGYLIHV
                     GVLVMGIGVIGSLNYQQQTQRSLAPGGTVTIGAYTIRFDRSLQCDPSASMPAGCQAWY
                     TDRGLDIAIQAASLTVSKSGQAPLELTPYQEIFLNGTGQLSPPGVLGSIAEDLYIIYL
                     GDQSGFATFKVYINPLISFLWTGGIILVLGTIVAMWPSALERRQTVSMPDRSRLAQRA
                     VK"
     gene            1186..1689
                     /locus_tag="VFF70_11825"
     CDS             1186..1689
                     /locus_tag="VFF70_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZY45427.1"
                     /translation="MRSRSIRRVTVFCGALIGVLTIVGVAVAQTGTPPAPKSVTPDDV
                     NRIARQLYCPVCENEPLDVCQTSACQQWRAQIGQLVAEGKTDPEIIQYFIDRYGLKVV
                     GAPPFSSETIWLYLLPVVGLIAGAIYVIFLMRRMRARGSAVADTPTSNESGDDYVKRV
                     ERDLKKW"
     gene            1794..2372
                     /locus_tag="VFF70_11830"
     CDS             1794..2372
                     /locus_tag="VFF70_11830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZY45428.1"
                     /translation="MTTLDNSTPSAIHRSKSLKRYLPIGLVVLFVAFMAFGLSLRNRS
                     EPTSGPAPDFTLTFYPGQDGGLDVPQIKLSDLRGKVVLINFWASWCIPCAEEAPDLQA
                     TYLKYKDRGVVFLGIAWTDIEADAINFMRRFNITYANGPDIGNKIAKPIYQITGVPET
                     FIVDRTGNVQYFAISPMTESVLSTELDRILAQ"
     gene            2450..2944
                     /locus_tag="VFF70_11835"
     CDS             2450..2944
                     /locus_tag="VFF70_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY45429.1"
                     /translation="MQIVAILIGVFIAAGVGWYISRPLLQAKRMSTTDEPVQSMEVQR
                     DSLYAQISELELDHATGKTNDEDYQQVRTGLVTQAANVLREIDGQSPSAKTQDADIEA
                     LIAARRKTTVKPAGKADLDQQIEAAIKARRSGIVCPNCGKLATADDVFCSRCGTSLKI
                     PQKV"
     gene            2955..>3435
                     /locus_tag="VFF70_11840"
     CDS             2955..>3435
                     /locus_tag="VFF70_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZY45430.1"
                     /translation="MKRFFSITLLLLAVVVLAGCSLAAPAPTAPITGPIAQSPTQSAN
                     IEPVQLPTRRPDANAGAAIYAAKCIDCHGQQGHGDGSKAEQVRSQTGTAPADLTSDPI
                     ARAQSPEQWYNQITNGQLDKLMPPFAQSLSPDQRWDVIAYLWTLAASPGEITQGQSIY
                     "
BASE COUNT          751 a          929 c          942 g          813 t
ORIGIN      
        1 gatcatcggc acgctggcga cacgcaccgg cttgctttct tccgtgcacg ccttcgctcg
       61 atcgtcgttg tctgtaccgt tcttggcgtt tgtcggattt gtcctcgtcg gctcgacggt
      121 tttgatgatc atccgatcgg atgtattgaa gagcgacaat aaactcgatt cgatcttctc
      181 gcgtgaagcg gcctttgtct tgaacaactt cttgttcgtg acgatcacct tcaccgtctt
      241 tatcggcacg tggtattcgt tgatctccga agcgttgatc ggtgaaaaac tcaccgtcgg
      301 cccggcggtc tacaatcaaa tgttggggcc gcaggtggcg ctgctcattc tgctgatggg
      361 tatcggcccg ctcgtggcgt ggcgtcgatc gtcggcggaa gcattaggcc gcatgagttg
      421 gaagtcggcg ctgctggcga tggccgtgat gatcgcattg tacctgagcg gcattcaagc
      481 gatctgggtt ctggtcggtt tcggcttgat cggttacacc tttggcgtga cgatcagtga
      541 atactggcgc ggcgcgcggg cgcggcataa actatcgggc gaaaattatt tcattgcgct
      601 cgttagattg ttccaacgca atcgacgccg ctatggtggt tacttgattc acgtcggcgt
      661 gttggtgatg ggcatcggcg tcatcggctc attgaattat cagcagcaaa cgcagcgttc
      721 actcgcaccg ggcggcaccg tcacgatcgg cgcgtacacc atccgcttcg atcgatcctt
      781 gcaatgcgat ccctctgcat cgatgccggc gggttgccaa gcatggtaca ccgatcgcgg
      841 tctggacatc gccattcagg ctgcatcctt aacggtctcg aagtccggtc aagcgccgct
      901 tgaactgacg ccgtaccaag aaattttcct gaatggcacc ggccagctca gcccgcccgg
      961 tgtgctcggc tcgatcgccg aagatctcta catcatttat ctaggcgatc aaagcggctt
     1021 tgcgactttc aaagtctaca tcaatccatt gatcagtttc ctgtggacag gcgggatcat
     1081 tttggtgcta ggcacgatcg tggcgatgtg gccttcggct ttagagcggc gtcaaactgt
     1141 ttctatgccc gatcgatcgc ggctggctca acgggcggtg aagtgatgcg ctcacgatcg
     1201 atcaggcgag tgacggtttt ttgtggcgcg ctgatcggcg tgttgacgat cgtgggcgtg
     1261 gccgtggcgc agactggtac gccgcccgcg ccgaagtccg tcacgcccga tgatgtgaat
     1321 cgcatcgcgc ggcagttgta ttgtccggtg tgcgaaaacg aaccgctcga tgtgtgccaa
     1381 acgtccgcgt gtcaacagtg gcgcgcacag atcggccagt tggtcgcaga aggcaagacc
     1441 gatccggaaa tcattcaata cttcattgat cgctatggct tgaaagtagt tggtgcgccg
     1501 ccgttttcca gtgaaacgat ctggttgtat ctgctgccgg tcgtcggctt aatcgccggt
     1561 gcgatctatg tgatcttcct gatgcgccgt atgcgggcac gcggaagtgc tgtcgccgat
     1621 acgccgacat cgaacgaaag tggcgacgac tatgtaaagc gcgtcgagcg tgatttgaag
     1681 aaatggtaaa cgacttcagg tcattctgag tcgtgcagag cacgccgaag aatctctatc
     1741 gatgatcaga gagattcctc gctgcgctcg gaatgacaac ttaagaactt agtatgacga
     1801 cactcgataa ttcaacccca tctgcaattc accgatcgaa atccttgaaa cgctatctgc
     1861 cgatcgggtt ggtggtgctc tttgtggcgt tcatggcctt tggcttgtcg ctgcgcaatc
     1921 gatctgaacc gacatccggc cccgcgcccg atttcaccct gactttctat ccaggtcagg
     1981 atggtggctt ggacgtgcct cagatcaaat tatcagattt acgcggtaaa gtagttctga
     2041 tcaatttctg ggcatcctgg tgcataccct gcgctgaaga agcgcccgat cttcaagcga
     2101 catatttgaa gtataaagat cgtggcgtgg tatttttggg tattgcttgg acggacatcg
     2161 aagcggatgc catcaatttt atgcggcgtt tcaacattac atatgcgaat ggacctgata
     2221 tcggcaacaa aattgcaaaa ccgatatatc aaattaccgg tgtgccggag actttcatcg
     2281 tcgatcgcac tggcaatgtg cagtattttg ccatcagccc catgacagaa tccgtgttgt
     2341 caaccgagct cgatcggatt ttagcgcaat aaattcagta caagaataac acaaatgtca
     2401 cgaatcaaaa tcatattcgt gggattcgtg gacaaggaat ctggttacta tgcaaatcgt
     2461 cgcgatcctc attggagttt tcatagccgc cggagtgggc tggtatatca gccgaccgtt
     2521 gctgcaagcg aaacgaatgt ccacgaccga tgagcccgtt caatcgatgg aagttcagcg
     2581 cgattcgttg tatgcgcaaa tcagcgaact cgaactcgat cacgccacag gcaaaaccaa
     2641 cgacgaagat taccagcagg tgcgcaccgg acttgtcaca caagcggcta atgtgctgcg
     2701 cgagatcgat ggacaaagtc catcggccaa gacacaggac gcggatattg aagcattgat
     2761 cgctgcgcgg cgcaagacca cggttaagcc cgccgggaaa gccgatctcg atcaacagat
     2821 cgaggctgcg atcaaggcgc gtcgatccgg catcgtatgc cccaactgcg gcaagctcgc
     2881 caccgccgac gatgtgttct gttcgcgctg tggcacatcg ctcaaaatcc ctcaaaaggt
     2941 ataagcggtt cagcatgaaa agatttttct cgattacatt actgttactg gctgtagtcg
     3001 tactcgctgg ctgttcactg gccgcccccg cgccgacggc tcccatcacc ggaccgatcg
     3061 cgcagtcgcc cacccaatcg gctaacattg aacctgtgca gctgcctacc cgtcgaccgg
     3121 atgccaatgc aggcgcggcg atctatgctg ctaagtgcat cgactgccac ggccaacaag
     3181 gtcatggtga tggctcaaaa gccgagcagg tccgatcgca aacaggcaca gcgccggccg
     3241 atctcaccag tgatccgatc gcgcgcgcgc aatcgcctga gcaatggtat aaccagatca
     3301 ctaacgggca gctcgacaaa ttgatgccac cttttgcgca atctcttagc cccgatcaac
     3361 gctgggatgt catcgcttat ttatggacgc tggctgcatc accgggtgag atcacgcaag
     3421 gccaatcgat ctaca
//
LOCUS       DASRTJ010000581         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_74477,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000581 DASRTJ010000000
VERSION     DASRTJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74477"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFF70_11845"
     CDS             <1..489
                     /locus_tag="VFF70_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY45431.1"
                     /translation="AYALVVLPQKHFSALSGGVVLSFIMLPIIIRTTEEMLRLVPGTL
                     REGSLALGAPEWKTSWRVVLPAAWNGVLTGLLLAISRAAGEAAPMLFTSFGNPFMSTD
                     ISQPIATLPHTIFVYAIAPYDDWHAKAWATALVLIVLVLILNVAARLIVAWRARKLGV
                     VR"
     gene            572..1360
                     /gene="pstB"
                     /locus_tag="VFF70_11850"
     CDS             572..1360
                     /gene="pstB"
                     /locus_tag="VFF70_11850"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZY45432.1"
                     /translation="MSTPADLPIKMQAENLSVYYRSFRAVADVQLLINAKMITAIIGP
                     SGCGKSTLLRTLNRMNDLVAGSRVEGEVSLDDEPLYGPGIDVVDVRRRVGMVFQRPNP
                     FPKSIFDNVAYGPRLYGLHSKHELQEVVEKSLRRAALWDEVKDKLQESGMALSGGQQQ
                     RLCIARALAVEPEVLLMDEPCSALDPVATLKIEELMQQLKADYTIVIVTHNMQQAARV
                     SDFTAMMMMRPDRAGELIEFDATPIMFTNPKDKRTEDYVTGRFG"
     gene            1470..1910
                     /locus_tag="VFF70_11855"
     CDS             1470..1910
                     /locus_tag="VFF70_11855"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZY45433.1"
                     /translation="MPQEKYRVLVLCTGNSARSQMAEGLINAQLGDRFAAQSAGTQPI
                     GYVHPTTIVVMREIGIDIAQHRSKSVDEFIDQEFDIVITVCDDAAENCPVWLGRGFKT
                     HIGFPDPAAAPIQQQPEQFRIIRDAMQDRILNFLKAWQPAMGRM"
     gene            1910..2578
                     /gene="phoU"
                     /locus_tag="VFF70_11860"
     CDS             1910..2578
                     /gene="phoU"
                     /locus_tag="VFF70_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZY45434.1"
                     /translation="MTTSTIRSALDRAMHKVSDDILRLGSMVENAIDRSVSALKQRDS
                     KLAQQVIEEDQQINAMRFQIEEECLELIATQQPAASDLRATIAATHIAVELERMADHC
                     TGIAEITIRMQGQPLLKPLIDIPLMATVAKEMTRGALDAFVRRDADLAKSIVKRDDEM
                     DELYQQIFRELLTYMLEDPKNISRATFLLWVAHNLERIGDRATNLCERTIFISTGQLG
                     DLNV"
     regulatory      2690..2808
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2971..>3285
                     /locus_tag="VFF70_11865"
     CDS             2971..>3285
                     /locus_tag="VFF70_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45435.1"
                     /translation="MTLNEQVQLTAALTVSGSGQQTRRMPIHAARLLALPLTFAVIIL
                     IWLLIIRLGNYPAFILPSPGDVWTELIRSIQTASFWGHIGVTLIEVLGGLVIGAALAI
                     VLG"
BASE COUNT          730 a          869 c          939 g          747 t
ORIGIN      
        1 gcttatgcgt tggttgtctt accacagaaa catttctcgg cattatcggg cggcgtagtg
       61 ctgtccttta tcatgctgcc aatcatcatc cgcacgaccg aggaaatgct gagattagtg
      121 cccggcacac tgcgcgaagg atcgctggcg ctgggcgcgc ctgaatggaa aacttcatgg
      181 cgcgtcgttt tgcccgcggc ttggaacggc gtattgaccg gcttgttgct cgccatttca
      241 cgtgccgccg gtgaagccgc gccgatgttg ttcacatcgt tcggcaatcc atttatgagc
      301 accgacatca gtcagccgat cgccaccctg ccgcatacga tttttgtgta tgccatcgcg
      361 ccgtatgacg attggcatgc aaaggcgtgg gccaccgcgc tcgtgctgat tgtgttggtt
      421 ttaatattga atgtcgcagc aagattaatc gtcgcgtggc gcgcccgtaa gctgggcgtc
      481 gtccgttaag tctattattt agaagaggtt agtgatggga caccatgtaa cgctgggaag
      541 caccttagcc gtcaacagcc aagccgccga aatgtctacg cccgcggatt tgccgatcaa
      601 gatgcaagct gaaaacttat cggtgtacta tcgttcgttc cgggccgtgg ctgatgtcca
      661 gctgttgatc aatgccaaaa tgattacggc gattatcggc ccctcaggct gcggcaaatc
      721 gactttgttg cgcaccttga atcgcatgaa tgatttggtt gcaggctcgc gcgtcgaggg
      781 tgaagtctct ctggatgatg aaccgctcta cgggccgggc attgacgtgg tcgatgtgcg
      841 ccgccgcgtg gggatggtct ttcaacggcc caatccgttt ccgaaaagta tcttcgataa
      901 cgtcgcgtac gggccgcgct tgtatggcct gcacagcaag cacgaactcc aagaagttgt
      961 tgaaaaaagt ttgcggcgcg ccgcattgtg ggatgaagtc aaagacaaac tgcaagaatc
     1021 gggcatggcc ctgtccggcg ggcaacagca gcgcttgtgc attgcccggg cattggcggt
     1081 ggaaccggaa gtcttgttaa tggatgagcc gtgctcagcg ctcgatccgg tggcaacctt
     1141 gaagatcgaa gaattgatgc agcagctcaa agcggattac acgatcgtga ttgtgacgca
     1201 caacatgcag caggccgcgc gcgtgtccga tttcacggcg atgatgatga tgcgccccga
     1261 tcgagccggt gagctgatcg aatttgatgc gacgccgatc atgtttacca atcccaaaga
     1321 caagcgcacc gaagattacg tgaccggacg atttgggtaa agaaggctca aggcggagcg
     1381 ctagctccgc cgccggagct ggcgctccag caggagcaac gctaaattga ttgacccatt
     1441 agattgagag tgctacaatg agcttgaaca tgccgcagga aaaatatcgg gtcttagttt
     1501 tatgtaccgg caactcggcg cgatcgcaaa tggccgaagg tttgatcaac gcgcagctgg
     1561 gtgatcgatt tgcagctcaa tcagccggca cccaaccgat cgggtatgta catcccacca
     1621 cgattgtggt gatgcgcgag atcgggatcg atatcgcgca gcatcgatcg aaatcagtcg
     1681 acgagtttat cgatcaagaa tttgatatag tgatcacggt ctgtgatgac gccgccgaaa
     1741 attgtccggt gtggctgggg cgcggtttca aaacacatat tggatttccc gacccggccg
     1801 ccgcgccgat tcaacaacaa cctgaacagt tccgtatcat acgcgacgca atgcaggatc
     1861 gaattttgaa tttcttaaag gcatggcagc ccgcgatggg gaggatgtga tgacaactag
     1921 caccattcgc agcgcactcg atcgtgcgat gcataaagtc agcgatgaca tcttgcggct
     1981 cggcagcatg gtcgagaatg cgatcgatcg atcggtctcg gcactcaagc agcgcgacag
     2041 caaacttgcg caacaagtca tcgaggagga ccagcagatc aacgccatgc gctttcagat
     2101 cgaagaggaa tgtctggaac tgatcgccac gcagcagccg gccgccagcg atctgcgcgc
     2161 gacaattgcc gcgacgcaca tcgccgtgga gttggagcgc atggccgatc actgcaccgg
     2221 catcgccgag atcactattc ggatgcaggg tcagccgctg ctcaagccgt tgatcgatat
     2281 tccgttgatg gcgaccgtgg ccaaagagat gacgcgcggc gcgctggatg cgtttgtgcg
     2341 gcgcgatgcc gacctcgcca agtcgattgt caaacgcgac gacgagatgg atgaactgta
     2401 tcaacaaatt ttccgcgaac tgttgacgta catgctggaa gacccgaaga atatcagccg
     2461 cgccacgttt ctgttgtggg tcgcgcataa tctggagcgc atcggcgatc gcgcgacgaa
     2521 tctgtgtgag cgcacaattt ttatctcgac tggccagctc ggcgatttga atgtgtaatc
     2581 tgtacgggcg aggtaacctc gcccgtactt tttgtcatat ggaatccgcg atcgatctat
     2641 attgacacat tctacccagc tgttataatt tcgtcgtaac caattgaata gcaaccttcg
     2701 gggcagggtg aaattcccga tcggtggtac agcccacgag ttcctcacgg aacatgattc
     2761 ggtgtaattc cgaggccgac ggtacagtcc ggatataaga aggttcacga ttcgcgcgca
     2821 ggcgtgtctg tttgcgtatc gatctgcccc ggagccagtt gtttttggct tcggggtttt
     2881 tgatcaagca gcgcttcaca cgctcgacgg ctgtggcccg ccccgaagga catctgacct
     2941 tacggggtat tattttagcg aggcaaccgc atgaccctga atgaacaagt tcaattaacc
     3001 gcagcgctga ctgtttcggg atccgggcag caaacgcggc gaatgccaat acatgccgcg
     3061 cgtttgctcg cgctgccact caccttcgcc gtcatcattt tgatctggct gttgatcatt
     3121 cgccttggca actacccggc cttcattctg ccttcacccg gcgacgtgtg gaccgaactc
     3181 atacgctcaa ttcaaacggc ttcattctgg ggacacatcg gcgtcacgtt gatcgaagtg
     3241 ctgggcggac tcgttatcgg cgcggcgttg gcgattgtct taggc
//
LOCUS       DASRTJ010000582         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_280684,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000582 DASRTJ010000000
VERSION     DASRTJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280684"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFF70_11870"
     CDS             complement(<1..899)
                     /locus_tag="VFF70_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZY45436.1"
                     /translation="MLLWDFIADRDTPARKKLIADLKVDLQIRLSDQARVIDSFSERE
                     LYSRDLGDLPKWIEKAFFRTTPHLVLQPIDATQVAIILKFSVEKNLPIIPRGIASTAF
                     GGPVPTKNGLVIDFSPMKKIGSISVGADPTITVQAGARFGDIEDFIQPYGYTLAVYPS
                     NRLGTIGGWLSSGGYGMNALKYGHVREWIASIEVVTMDGSIRWLKNGEPDFDAFIGTE
                     GQMGIVTQVMLRLLPRPEVRVPHLLGFDSAKAAFDFANEIYARDSVPATITYLSSELL
                     RLLNQLHEIDHHDSLSREAGEGQG"
     gene            1466..1813
                     /locus_tag="VFF70_11875"
     CDS             1466..1813
                     /locus_tag="VFF70_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZY45437.1"
                     /translation="MTKLMMFTRTFSCSDLELARNCLAELNVHPIELNISIDRDAAQT
                     LMEWVGSLSVPTLVVTDDSDQPIDPPQTLQPDQHTRNTDRGSMISEPNCVSLRLFLKK
                     HGFISDHDHTSNN"
     gene            1791..>2338
                     /locus_tag="VFF70_11880"
     CDS             1791..>2338
                     /locus_tag="VFF70_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45438.1"
                     /translation="MITHQTISIEGRSAKVWMGGSGRPLVLLHGGFGDAQQHWHATFQ
                     AFAPHFHIMAPDLPGFGVSAPLAVPSFQSYLSWINVLFEMLGLAGPMLMMGNSFGAAI
                     ARLYAAANTGEIDRLVLIDGGAIQDVGGCARSIFQLPIVSPFMLEMARRQSFSNKGLR
                     KAIHVDSVFTPEFAANAQAASHG"
BASE COUNT          553 a          617 c          625 g          543 t
ORIGIN      
        1 ccctgaccct cccccgcttc gcgggagagg gaatcatgat gatcaatctc atgcagttga
       61 ttcagcagac gaagcaactc tgaggacaga taggtgatcg ttgctggaac actgtcacgc
      121 gcgtaaatct cattggcaaa atcaaaagcg gctttagccg aatcaaagcc taatagatgc
      181 ggcacgcgca cttcggggcg aggcagcaag cgcaacatca cttgggtgac gatgcccatc
      241 tggccttcgg tgccgatgaa tgcatcgaaa tcaggctcac catttttcag ccagcggatc
      301 gatccatcca tggtcaccac ttcgatagat gcgatccatt cccgcacatg accgtacttc
      361 aatgcattca tgccgtaacc gccgctcgac aaccagccac cgattgtgcc caagcgattt
      421 gaaggataga cggctaaagt gtaaccgtag ggttgaatga aatcttcgat gtctccgaaa
      481 cgcgcgccgg cttgcacagt gatcgtcgga tcagcgccaa ccgagatcga tccgatcttt
      541 ttcatcggcg aaaaatcaat cacgaggccg ttcttggtcg gcaccgggcc gccaaaggcg
      601 gtcgaagcaa tgccgcgcgg aatgatcggc aggtttttct caacggagaa tttcagaatg
      661 atagccactt gcgtggcatc gatcggttgg aggaccagat gcggcgtcgt gcggaagaag
      721 gctttctcga tccacttggg gagatcgccg agatcgcgcg aatacagttc acgctcggaa
      781 aacgaatcga tcacgcgggc ttgatcgctc aatcggattt gcagatcaac ctttagatcc
      841 gcgatcaatt ttttgcgcgc gggcgtatct cgatcggcaa taaaatccca tagcaacata
      901 gggttctccc atcaatcggc cagcataata aacggcgctt aatataaaac ggttttccca
      961 atgcccggct tggcacattg gctgacaaca acaacaaaac aaaagtggat tcaactgcca
     1021 ctgcgcacgg cttcaccgtg aggctagtca gtgtgcttga agaattttgg gggaaagcac
     1081 aggattgtgg cgaccctgag aggcatccgc tgatcgttcg attttccccc gcgtgcgcgg
     1141 ggttagctgc acctgcggtg cagaacctcc acctcgttgt ctcgccaacg cgagacccat
     1201 acgctagctt cccatttcat tgaaaattaa atcagtcgtt cacctctgca ttgaagatgt
     1261 gctgagtata agcgaacttt taggcggcgt caagatgcaa aatgcggggt aggaaacata
     1321 ttcggtataa gtccatgttc gattgaaatc gggcgcgctg ctagtgcacc gtttgacccg
     1381 ctgtatgtgc gaatgtgtcg tccttaagaa ttaatgtaaa gaatttggta tgtctcgtcc
     1441 aactggattc aggtataatc gtaacatgac gaagttaatg atgttcaccc gcacgtttag
     1501 ctgcagcgat ctagagctgg cgcggaactg tctggccgag ttaaacgtcc atccgattga
     1561 gctcaacatc tcgatcgatc gggacgcggc gcagacatta atggagtggg tgggatcgtt
     1621 gagcgtgccg acgttagtcg tgaccgacga tagcgatcaa ccgatcgatc cgccgcagac
     1681 cttgcagccc gatcagcaca ctcgcaacac cgatcgcgga tcgatgatca gtgaacccaa
     1741 ctgcgtttca ctgcgactct ttttaaagaa acacggattc atttctgatc atgatcacac
     1801 atcaaacaat tagcattgaa ggccgatcgg ctaaagtctg gatgggcggc agcggccggc
     1861 cgttggtgtt gctacacggc ggtttcggcg atgcccaaca acattggcac gccacttttc
     1921 aagctttcgc cccgcacttc cacatcatgg cgccagatct gcccggcttt ggcgtcagcg
     1981 cgccattggc cgtgccgagt tttcaatcgt atctcagttg gatcaatgtg ttgtttgaaa
     2041 tgttaggcct ggccggtccg atgttgatga tgggcaattc attcggggcg gcgatcgccc
     2101 gcttgtatgc cgccgcaaat acgggcgaga tcgatcgatt ggtgctgatc gacggtggtg
     2161 ctattcaaga tgtgggtggc tgcgctcgat cgatcttcca actcccgatc gtcagtccgt
     2221 tcatgctgga aatggcgcgg cgccaatcgt tttcgaataa gggtctgcgt aaagcgatcc
     2281 atgtcgattc ggtcttcacg cctgagtttg ccgcgaatgc tcaagccgcc tcgcacgg
//
LOCUS       DASRTJ010000583         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_339657,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000583 DASRTJ010000000
VERSION     DASRTJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339657"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFF70_11885"
     CDS             <1..643
                     /locus_tag="VFF70_11885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45439.1"
                     /translation="EHGAEVIQHPFIDYASQRNEAMNMIDAEWIFFVDADERSTPELA
                     EEINLIVAHGGRESAGLTSGESIAGWFVPRKNYLFDKLTRGAGYWPDYQMRLLKRGYA
                     RYERPASEIVKLDGQSGYLKNPLIHYNYESIAQFHEKQTFRIDFEATNLFKQGIKPKF
                     YTPYTQYVRHFLWRFITLKGYQDGFHGMRLSMLLAYYFGYRYYRRLAELWKNS"
     gene            858..>1780
                     /gene="aspS"
                     /locus_tag="VFF70_11890"
     CDS             858..>1780
                     /gene="aspS"
                     /locus_tag="VFF70_11890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZY45440.1"
                     /translation="MYKTHNCGELRLTQAGQKVTLAGWLHKRRDHGGLIFIDLRDRTG
                     LAQITIDPSVKAAYEVAEKARAEFVLKIVGTVRARPAGLANPNMQSGEIEVLAESIEI
                     LNPAKPIPFPIDDDGMKTTEALRLKYRYLDLRRARLQKNLILRHKTIQFIRNYMDGQG
                     FIEIETPMMIKSTPEGARDYVVPSRVQPGKFYALPQSPQQLKQLLMVAGYEKYYQIAR
                     CFRDEDLRGDRQPEFTQLDLEMSFADRDDVLAVVEGLVTGLVPAVAPHKRILSPLPRI
                     TYRDAMARFGSDKPDLRFGMELIDLTEILQGS"
BASE COUNT          421 a          427 c          511 g          421 t
ORIGIN      
        1 tgagcacggt gcagaggtga tccagcatcc atttattgat tacgcctcgc aacgtaatga
       61 ggcgatgaat atgatcgatg ccgaatggat cttcttcgtc gatgccgatg aacgatcgac
      121 gccggagttg gcggaagaga tcaacttgat tgtggctcac ggcggacgag agtccgccgg
      181 attgacatcc ggtgagagca ttgcaggctg gtttgttcca cgcaaaaatt atctcttcga
      241 caaactcacg cgcggcgcag gctattggcc cgattatcaa atgcgtttgc tgaaacgcgg
      301 ttacgctcgg tatgagcgtc ccgcatctga gatcgttaag ctggatggcc aatcgggtta
      361 tctgaagaat ccattgattc actacaacta cgaatcgatc gcacagttcc atgagaagca
      421 aacattccgc atcgacttcg aagcgacgaa tcttttcaag caaggtatta agccgaaatt
      481 ctatacgcct tacacgcaat acgtccgaca ttttttgtgg cgcttcatca ccttgaaagg
      541 ctatcaagat ggttttcacg gaatgcgatt gtcaatgctg ttggcgtatt attttggtta
      601 tcgctactac cggcggttgg ccgagctgtg gaaaaattcg tgattgactt tccggtcaaa
      661 gtgcatataa tcagaggcat gatgcgagag attattgtgg ttgatggcgc gaacgataat
      721 aacgatgatg ccgatgggcg tcgatcgatt tcgtcgcgcg cgtagcacct acttgttttt
      781 ctgacgaaaa tgacagccgc ccttccgcta agggcggttt tgttttatct tgcaacccga
      841 ccaataagga gccgatcatg tataagacac acaattgcgg tgaacttcga ttaactcagg
      901 cgggacagaa agtgacgctg gctggttggc tgcacaagcg gcgcgatcac ggtggtttga
      961 tctttatcga tctgcgcgat cgaacggggc tcgcgcagat cacgatcgat ccaagtgtga
     1021 aagcggctta cgaagtggct gagaaggcgc gcgccgaatt tgtgttgaag atcgtcggca
     1081 ccgtgcgcgc ccggccggcg ggcttggcga atccgaacat gcagagtggc gagatcgaag
     1141 tgctggccga atcgatcgag attctgaatc cggcgaaacc cattccattt ccgatcgacg
     1201 acgacgggat gaaaacgacc gaagcgctgc gcttgaagta tcgctatctt gatctgcgcc
     1261 gcgcgcggct gcagaagaat ttgattttgc gccataagac gattcagttc attcgcaact
     1321 acatggatgg gcagggcttt atcgagatcg aaacgccgat gatgatcaag tcgacgccgg
     1381 aaggcgcgcg cgactatgtc gtgccaagcc gggtgcagcc cggtaaattc tatgcactgc
     1441 cgcaaagtcc gcagcagttg aagcagctgt tgatggtggc cggctacgag aaatattatc
     1501 agattgcccg ctgtttccgc gatgaagacc tgcgcggcga tcgacaaccg gagttcacgc
     1561 agttggattt agaaatgtcg tttgccgatc gcgatgacgt gctggctgtg gttgaaggct
     1621 tggtgaccgg gctggtgcca gcggtggcgc cgcataagcg gatcctttca ccgctgccac
     1681 gcatcactta ccgggatgca atggcaaggt tcggcagtga caaacccgat ttgcgcttcg
     1741 gcatggagtt gatcgatctg acggaaattc tgcaaggatc
//
LOCUS       DASRTJ010000584         5285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_111559,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000584 DASRTJ010000000
VERSION     DASRTJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111559"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4889
                     /locus_tag="VFF70_11895"
     CDS             <1..4889
                     /locus_tag="VFF70_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45441.1"
                     /translation="AEITGNIIIGKLPEHAFNESDVRLLTTLASSMNVALENARLFDE
                     TQRLLKETEERNAELAVINSIQEGLVSELNFQAIIDLVGNKVQEIFNVSEVEIALYDA
                     ATQMISFPFWSTPEGQAHAEPLPLGTGMMSHVIQTREPFILTDENREVVMQKAVIPAG
                     LQHRKSFIGAPIISGGEALGAISLHDSSDEHAYDESDLRLLTTIANSMAVALKNARLF
                     DETNRLLKETDQRAVELAIINSVQEGLASKLDMQSIYDLVGDKIREVFDAQGVAINYY
                     DRTTNYIKSPYYLFRGERIYGFDLEYGQGMTTHIIESGQSILINENAVERYRELGTVF
                     ARGEEENTAKSWLGVPLIAGGNVTGAIHLENYEREHAFSQSDVRLLQTLANAMSAALE
                     NARLLDETQRLLKETDRRAAELAIINSVQEGLASKLDAQAIFDLVGDKINAVFDAHAI
                     QIITYDRAANLVDYRYQIEKGARQFVEPREPGGFSGHILQTRQPLLINEHMDQRRAEF
                     GSAVLVGNPAKSYLGVPLMLNGEVAGVLSLQNVDREQAFGENELRLLSTLSATMAAAL
                     DNARLFDETNRLLKETDQRAAELAIINGVQEGLASKLDMQSIYDLVGDKLCEVFDSQN
                     MDIRLFDPATGLVNFPYVRDRGERLQVEPMPIRGVSKAVIESGQPLMVAQDMAQHMAE
                     LGSTIIPGTDVEKSFVAVPIVSGDQTIGLVYLGSYEKENAFGDSDVRLLQTVVSSMSV
                     ALENARLFDETQRRASEMSALTEIGRQISATLDLTSVLDQITRNTRDVLQASTSAVYL
                     LDPDGLTLRPIAAIGEVAEAVKNYSPQLGQGLIGSIAASGEAEAIANTFKDPRTVVLP
                     GTAENDEGEKLMVAPLFAREQVIGVMAVWRNPGDPIFTQEDLDFVIGISRQASIAIQN
                     ARLFEETERRARESAATGEILRIISQSPGDERPVLKSIAEYAQRLCEADDAFIVRAEG
                     ERLVPVSSEVRSGAAVEHISVPLDRRMVGGRAHLERRTVQVSNLAEASDEEWGLVKER
                     ALSIGIQTILATPLLRENESLGVIVIQRRSVEPFSDKQVALIETFADQAVIAIENARL
                     FDEKEQARAEAETANQAKSAFLATMSHEIRTPMNAVIGMSGLLLDTPLNTEQREFAEI
                     IRNSGDALLTIINDILDFSKIEAGKMELEAQPFDLRECVEGTLDLMAARASEKKLDLA
                     YTFDDAVPNAIVGDVTRLRQIVLNLLSNSIKFTAQGEVVLQVSVDLYVDPLAGDRSGL
                     QQLHFAVKDSGLGIPPDRMNRLFQSFSQVDASTARKFGGTGLGLAISKRLSELMGGNM
                     WAESEGIPGQGATFHFTILAKSAEQSASTRRELRGDQPQLKDKRVLIVDDNDTNRRII
                     TLQLHKWGMLTRDTASTREALNWIAQNEAFDVAILDMHMSEMDGIELAKEIRKQRDAK
                     KLPLVLFTSLGRREADAESIGFAAHLNKPLKPSALFDAMISIFTDQGARLTAPSAPVK
                     LQMDPDMAKRLPLKILLAEDNAVNQKLALRLLQQMGYRADVAGNGLEAVEAIERQKYD
                     VILMDVQMPELDGLDATREIRKLKIAAQPRIIAMTANAMQGDREMCIAAGMDDYISKP
                     IHIEELVNALNHAQVEKH"
     gene            5032..>5285
                     /locus_tag="VFF70_11900"
     CDS             5032..>5285
                     /locus_tag="VFF70_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45442.1"
                     /translation="MKKTDKPKKTVALSKTTAKPKSRSARKPTRAVKHAVHTVGQRTK
                     ATMDLRVLELQASADRASQALEFAAAIDQYTQALDLLRGAG"
BASE COUNT         1228 a         1419 c         1529 g         1109 t
ORIGIN      
        1 gcgctgaaat aacgggcaac attatcattg gcaagctgcc tgagcatgct ttcaatgaat
       61 ctgatgtgcg cttactgacg acgctcgcct cctccatgaa cgttgcgctg gaaaacgcgc
      121 ggctctttga tgaaacccag cggctcttga aagaaaccga agagcgcaac gcggaactgg
      181 ctgtcatcaa cagcattcaa gagggcctgg tctctgaact gaattttcag gccatcatcg
      241 atctggttgg caacaaagtg caggaaatct tcaacgtgtc cgaagttgaa atcgccttgt
      301 atgacgcggc cacgcaaatg atctcgttcc ccttctggtc cacacctgaa gggcaagccc
      361 atgccgagcc gctgccgtta ggcaccggta tgatgtcgca cgtcattcag acgcgcgagc
      421 ctttcatctt gaccgatgaa aaccgggaag tggtaatgca gaaagcggta attccagccg
      481 ggctgcaaca tcgaaaatcc tttattggcg cgccgatcat ttcgggcggc gaggcgcttg
      541 gtgctatctc gctgcatgat tccagcgacg agcacgccta tgatgaatcc gatttgcggt
      601 tgctgacaac gattgccaac agtatggctg tggcacttaa gaatgcgcgc ctgttcgatg
      661 aaaccaatcg gctgttgaaa gaaaccgatc aacgcgccgt cgaactggcg atcatcaaca
      721 gcgtgcaaga aggtctggcc tccaaattgg acatgcaatc gatctatgat ctggtcggcg
      781 acaagattcg tgaggtcttc gatgcccaag gcgtagcgat caattattac gatcgtacaa
      841 ccaactacat caagtccccc tactatctat ttcgtggcga acggatatat ggttttgatc
      901 ttgaatatgg tcaggggatg acaactcaca tcattgagtc tggccaatcg attctcatca
      961 atgagaatgc agtcgaacgc tatcgggaat tagggactgt gtttgcacga ggagaagaag
     1021 agaatacagc aaaatcctgg ctgggtgttc ctctaattgc aggtggcaat gtgacaggcg
     1081 cgattcattt ggagaactac gagcgcgagc atgccttcag tcagtccgat gtgcgcctgc
     1141 tgcaaacgct tgccaacgcc atgagcgcgg cgctcgaaaa cgcccgcctc ttagatgaaa
     1201 ctcagcggct gttgaaagaa accgatcggc gcgcggccga actggcgatc atcaacagcg
     1261 tacaagaagg cctggcttct aagttggacg cgcaggccat cttcgatttg gttggcgaca
     1321 agatcaacgc agtctttgac gcgcacgcga ttcagatcat cacctacgat cgcgccgcga
     1381 acttggtgga ctatcgctat caaattgaaa aaggcgcgcg gcagtttgtc gaaccgcgtg
     1441 aaccgggcgg tttctccggt cacatcctcc agacgcgcca gccgcttttg atcaatgagc
     1501 atatggatca gcgccgggcc gaattcggca gcgcggtcct cgtcggcaat ccggccaaat
     1561 cgtatctggg tgttccgctc atgctgaatg gcgaagtggc tggcgtccta tcactgcaga
     1621 atgtcgatcg cgagcaggcc tttggcgaga acgaacttcg attattatca accctatcgg
     1681 caacgatggc cgccgcatta gataacgcgc gcctgttcga tgagacgaat cggttgctga
     1741 aagaaaccga tcagcgcgcc gccgaactgg cgatcatcaa cggtgtgcaa gaaggcctcg
     1801 cctcgaagtt ggacatgcag tcgatctacg atctggtcgg tgacaagttg tgcgaagtct
     1861 tcgattccca gaacatggac attcgcctgt tcgatcccgc aaccggcttg gttaacttcc
     1921 cctacgtgcg agatcgcggt gaacggctgc aggttgaacc gatgccgatt cgcggtgtct
     1981 ccaaagccgt gattgaatcg gggcagccgc tgatggtggc acaagacatg gcccagcata
     2041 tggccgaact tggctcaacc attattcccg gcaccgatgt ggaaaaatcg ttcgtggctg
     2101 tgccgatcgt atcgggcgat caaacgatcg ggttggtgta tctgggcagt tacgaaaaag
     2161 aaaatgcttt tggtgattcg gatgtgcgct tattgcaaac cgttgtgtca tcgatgagtg
     2221 tggcgctcga aaacgcgcgg ctgttcgatg agactcagcg ccgcgccagt gaaatgtcgg
     2281 cactgaccga gattggccgc cagatttcag ccacgctcga tctgaccagc gtgctcgatc
     2341 agatcacgcg caacacgcgc gatgtcttgc aggccagcac cagcgcggta tatttgttgg
     2401 accctgatgg gctcacgctg cgcccgatcg cggccatcgg cgaagtggcc gaggccgtga
     2461 aaaactattc gccgcagttg ggccaaggct tgatcggatc gatcgcggcg tcgggtgaag
     2521 ccgaagccat cgccaatacc ttcaaagacc cgcgtaccgt tgtgctgccc ggcaccgctg
     2581 agaacgatga gggcgaaaag ttgatggtcg cgccgttgtt cgcccgtgag caagtcattg
     2641 gcgtcatggc cgtctggcgc aatcccggcg atccgatctt cacgcaagaa gaccttgact
     2701 tcgtcatcgg catttcgcgt caggcttcga tcgccattca gaatgccaga ttgtttgaag
     2761 agaccgaacg ccgcgcgcgt gaatcggcgg cgaccggtga aatcttgcgg atcatcagcc
     2821 agtcgcccgg cgacgaacgg cccgtgctca agtcgattgc cgaatatgct cagcgcttgt
     2881 gcgaagcgga cgacgcgttc atcgtgcggg ctgagggtga gcggttggtc cctgtttcgt
     2941 cggaagttcg aagcggtgca gcggttgagc acatctccgt accgctggat cggcgaatgg
     3001 tgggcggtcg ggcgcatcta gaacgccgca cggtgcaggt ttcgaatttg gcggaagctt
     3061 ccgacgaaga atggggtctg gtcaaagagc gcgcgctgtc gatcggcatc cagaccattc
     3121 tggccacgcc gctgttgcgc gagaatgaat cactgggtgt catcgtcatt caacgccgat
     3181 ccgttgagcc gtttagtgat aagcaagtcg cgctgatcga aacgtttgcc gatcaagcgg
     3241 tgattgccat tgaaaatgcg cggctgtttg atgaaaagga gcaagcccgc gccgaagccg
     3301 aaacggccaa tcaagccaag agcgcgttcc tggccaccat gagccacgaa atccgcacac
     3361 cgatgaatgc cgtcatcggc atgagcggtt tgttgttgga cacgccgctc aacaccgagc
     3421 agcgtgagtt cgccgagatc attcgcaact ctggcgatgc gctgttgacg atcatcaacg
     3481 acatcctcga cttcagcaag atcgaagcgg gcaagatgga attggaagcc cagccgttcg
     3541 atctgcgcga atgcgtggaa ggcacgctcg atctcatggc ggcccgcgct tccgagaaga
     3601 aactcgatct ggcctacacc tttgatgatg ctgtgccgaa tgcgattgtc ggcgatgtca
     3661 cacgcctgcg ccagatcgtg ttgaatctgc tctcgaactc gatcaagttt actgcgcagg
     3721 gtgaagtggt gctgcaagtc tcggtcgatc tgtatgtcga tccattggcc ggtgatcgat
     3781 ccggtctgca gcagctgcac tttgcggtga aggattccgg tctcggcatc ccgcccgatc
     3841 ggatgaaccg gctattccaa tcgttctcgc aagtggacgc ctcgaccgcc agaaaattcg
     3901 gcggcaccgg cctcggcctc gccatcagca aacgcttgag tgaattgatg ggcggcaata
     3961 tgtgggccga gagcgaaggc attcccggtc aaggcgccac gtttcatttc acgattctgg
     4021 ccaagtctgc cgaacaatcc gcttcgacgc ggcgcgaact gcgcggcgat caaccgcagt
     4081 tgaaagacaa gcgcgtactg atcgtcgatg acaacgacac gaaccggcgc atcattactt
     4141 tgcagctgca caagtggggc atgctgacac gcgatacagc ctcgacccgt gaagcgttaa
     4201 actggatcgc gcagaatgaa gcgttcgatg tggcgatctt ggacatgcac atgtcggaga
     4261 tggatggtat tgaattagca aaagagattc gcaaacagcg tgatgctaaa aaacttccgc
     4321 tggttttgtt cacctcactg ggccgccgcg aagccgacgc ggaatcaatc gggtttgccg
     4381 cgcacttgaa caagccgctg aaaccgtccg cattgttcga tgcgatgatt agtatcttca
     4441 ccgatcaagg cgcgcggctg accgcgccgt ctgcgcccgt caagctacaa atggatccgg
     4501 atatggcgaa acggctgccg ctaaaaatct tgttggcgga agacaacgcc gtgaatcaga
     4561 agctggcgct gcgcttgttg cagcagatgg gttatcgcgc cgatgtggcc ggcaacggtc
     4621 tcgaagcggt tgaagcgatt gagcggcaga agtacgatgt gattttgatg gacgtgcaga
     4681 tgcctgagtt ggatggactc gatgcgacac gtgaaatccg caagttgaag attgccgcgc
     4741 agccgcgcat tatcgccatg acagccaacg ccatgcaggg cgatcgcgag atgtgcattg
     4801 ccgccggcat ggatgattac atcagcaagc cgatccacat tgaagagttg gtgaacgcac
     4861 tgaatcacgc gcaggttgaa aaacattaaa gggatgtcac acctgcgaag gccgatgtcc
     4921 agtcgccgcg ctggatttcc gctttcacgg gaatgacggc tagtcgcagc tcatgcagat
     4981 aatctgtgat cggcgtacaa taacaacgtc gtcacagtca aggctctcga tatgaagaaa
     5041 accgataaac cgaaaaagac cgtggctcta tcgaaaacaa cggccaaacc caaatcccga
     5101 tcggcgcgta agccgacacg tgcggtaaag cacgcagtgc ataccgtcgg gcagcgcacg
     5161 aaggccacga tggatctgcg tgtgctcgaa ttgcaagcca gtgccgatcg agcttctcaa
     5221 gcgttggagt ttgcagctgc gatcgatcaa tacacacagg ccttggactt gttgcgcggc
     5281 gctgg
//
LOCUS       DASRTJ010000585         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_111575,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000585 DASRTJ010000000
VERSION     DASRTJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111575"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFF70_11905"
     CDS             <1..186
                     /locus_tag="VFF70_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HZY45443.1"
                     /translation="TAMDDIYSELVTIDFPDAITGGLRRTTDTLRAVLERTRGDLTTT
                     LQQEKLQKALQEFQKKV"
     gene            198..500
                     /locus_tag="VFF70_11910"
     CDS             198..500
                     /locus_tag="VFF70_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45444.1"
                     /translation="MDTTDLNIICVFHGYFTSIMSERVIRASEIGQYDFCAKAWWLNV
                     IEGVPSENVGELQLGTQAHERHGQQVGRAGSLQQLAVVLLIAGVVLLAVFILGNGV"
     gene            509..1024
                     /locus_tag="VFF70_11915"
     CDS             509..1024
                     /locus_tag="VFF70_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZY45445.1"
                     /translation="MIWIAALLIVFGLIVLGIGRRAQAEAGLPIGRVIYADTGDWRAV
                     EKPLFSITYRLVGKPDYLIRRGRQVIPIEVKSSSAPVDGPRRSHLLQLAAYCLLIQEH
                     YHQRPKYGIVKYADRSFEVDYTPKLESHLLDVLDAMRNDLTNRLTHRSHAEPARCLRC
                     GVRSACSESLV"
     gene            1008..>1622
                     /locus_tag="VFF70_11920"
     CDS             1008..>1622
                     /locus_tag="VFF70_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZY45446.1"
                     /translation="MKVWYKMNVTDQLIAAKQAKEPQRNQPKPKHILLLNHPRVGASR
                     PLVNEMAEQLHAWGIQTHIASIGEDADDHNFAEHDMVIALGGDGTMLHTSRLTAMTGV
                     PVLGVNLGRLGFLAEVQPDEWRQVFDRVRQGDYWIEDRMMLHAEVWRGDEQRMSFEVL
                     NEVVVSRGALVKPVRLETYIDGGLLTTYVADGLIIATPTGSTAYA"
BASE COUNT          366 a          396 c          473 g          387 t
ORIGIN      
        1 actgcgatgg atgacattta cagtgagctg gtcacgatcg attttccgga tgccattacc
       61 ggcgggctgc gtcgcacgac ggatacttta cgcgccgtgt tagagcgcac gcgcggtgat
      121 ttgacgacga cgctacaaca agagaaattg caaaaggcac ttcaagaatt tcagaagaaa
      181 gtctagctgc gtttagcgtg gataccacag atttaaatat catctgcgta ttccacggct
      241 atttcacttc aatcatgtca gagcgagtta ttcgcgcaag cgagatcggc caatacgatt
      301 tttgtgcaaa ggcgtggtgg ttgaatgtca ttgaaggcgt tccatccgaa aacgtaggcg
      361 agctacagct cggtacgcag gcacatgaac gtcacggaca acaagtgggt cgcgctggaa
      421 gcttgcaaca acttgctgtc gtgctgctga ttgccggtgt tgttttactg gcggtgttca
      481 ttctgggcaa cggtgtgtag ttgatcgtat gatctggatc gccgcgctgc tcatcgtgtt
      541 tggtttgatc gtcctcggca tcggtcgacg cgcgcaagct gaggccggtt taccgatcgg
      601 gcgggtgatc tacgccgata ccggcgattg gcgcgcagtg gaaaagccgt tgttctcgat
      661 cacatatcgg ttggtcggca aacccgatta tttgattcgg cgaggccggc aagtcattcc
      721 gatcgaagtg aaatcgtcca gcgcgccggt tgatgggccg cgccgcagtc atctgctgca
      781 gctggccgct tattgtttgt tgatccaaga acattatcat cagcgcccga agtacggcat
      841 cgtcaaatat gccgatcgat cttttgaagt ggactatacg ccgaaacttg aatcacactt
      901 gctcgatgta ctcgatgcca tgcgcaacga tttaaccaac cgcctcacgc atcgatcgca
      961 tgccgaaccg gcgcgctgtc tgcgctgcgg ggttcgatcg gcgtgcagtg aaagtctggt
     1021 ataagatgaa tgtcactgat caactaatcg cggcgaagca agcgaaagag ccgcagcgca
     1081 atcaaccgaa acctaaacat attttgttgt taaatcatcc ccgagtaggc gcgtcgcggc
     1141 cgttagtcaa tgaaatggcg gagcagctgc acgcttgggg tattcaaact cacattgcct
     1201 cgatcggtga ggatgccgat gatcacaact tcgctgaaca tgatatggtc attgctttgg
     1261 gcggcgatgg tacaatgttg cacaccagcc ggttgaccgc catgaccgga gtgccggtac
     1321 ttggcgtaaa tttaggtagg ctgggctttc tggccgaagt tcagccggac gaatggcggc
     1381 aggtgttcga tcgggtacga cagggcgatt actggatcga agatcgcatg atgctgcatg
     1441 cggaagtctg gcgcggtgat gagcagcgta tgtcgttcga ggtacttaat gaagtggtcg
     1501 tcagccgcgg cgcactcgtc aaaccggtac gtttggagac atacatcgac ggcggtttgt
     1561 tgacgacgta cgtcgctgat ggtttaataa tcgccactcc caccgggagc acggcgtatg
     1621 ct
//
LOCUS       DASRTJ010000586         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_111595,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000586 DASRTJ010000000
VERSION     DASRTJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111595"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFF70_11925"
     CDS             <1..329
                     /locus_tag="VFF70_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45447.1"
                     /translation="IAARGSMRCFVGETNQIEIGERTNVQDGAIIHVDTCRPYLISSS
                     VTIGHASEVHRAVVDDDVLIGIGAKILSGARIGRECILGAGTLITGRTVLLPMSLVIG
                     LPGRLV"
     gene            complement(436..1188)
                     /locus_tag="VFF70_11930"
     CDS             complement(436..1188)
                     /locus_tag="VFF70_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45448.1"
                     /translation="MSSLVILGTAAAVHDALHENTYLVLKGARESILIDCCGNPLGRL
                     QRAGVSLSTLNTIILTHDHPDHTYGLPILLMDLWLLGRSTPLKIYAPASTIVTMKAIM
                     QAHAWADWPRFYPVEFKAIPDTIGAAVLENEEFTITAAPGVHFVPVVGLRVKNRSTGY
                     AIMYTSDTAPADPIKQLALGVNLLLHETAGATFGHSSAAMAGALAREAHVEKLILIHY
                     PVNADQAALVAEARSEFAGPIEISRDFAEYLF"
     gene            complement(1228..2724)
                     /locus_tag="VFF70_11935"
     CDS             complement(1228..2724)
                     /locus_tag="VFF70_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZY45449.1"
                     /translation="MAAITSTRTKLSWWGGVSILMRRELRDTFRDWRMVVPIVLLTLV
                     FPIIMTLTAGIAQNFVVRYGTPLIAERLFPFLLMIVGFFPISFSLVIALETFVGERER
                     KSLEPLLATPLSNGQLYLGKMLASMIPPLLAAYLGITVYLIGLLVFRGWSPPPILLLQ
                     VLILTTCEAIVMVSGAVVVSSQATSVRAANLLASFIIIPMALLIQAESVIMFLADYGV
                     LWGVVLALIVVDLILVRMGLSLFNREELLGREIDQIHPIGIGRKFKLFWLTANPGEAA
                     QRFSIKNMYHVHVPAAYRRVRSAVFSVIVVLIVSGIAGWIFVSRLHLPDDMLDQVREG
                     FTQLGGHAFDASAFARILPDAANNPALAVFWNNIRSLLIGGGLSMFSFGSIGVVMLMV
                     AVVPIALIAPVVGMAGLSPLTFILAYFVPHGVVELPAAILATAAGLRLGAALVHRTPK
                     LTIGEGWLLALMDFIKLFVFVVFPLLVLAALMEVYGTPKIVCWIYSCQ"
     gene            complement(2724..3656)
                     /locus_tag="VFF70_11940"
     CDS             complement(2724..3656)
                     /locus_tag="VFF70_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45450.1"
                     /translation="MIEAQGLSKSFGDFAAVRDVSLRVAAGEVLALLGPNGAGKTTTV
                     RMLSGILVPTAGWAKIAGLDIRKNAEEVRQRVGILTEMPGLYLRSTAREYLEFFGSVY
                     GLSTAQTRERIKTLMTQFEMSDVLNRRLSEFSKGMKQKLALIRCMLHDPDVLLLDEPT
                     SAMDPQSARLVRDALSTLRGDRRSIILCTHNLVEAESMADHIAIIRLGQIVIEGSPVE
                     LKTRLLGRPLMEIRFAEAMNGLLPSLHDRLDVESNGANWLRYWAANPISDNPPLLRWL
                     ADQRVNVVTLSEVPRSLEEVYLRVVEQPIMAEGY"
     gene            complement(3852..4460)
                     /locus_tag="VFF70_11945"
     CDS             complement(3852..4460)
                     /locus_tag="VFF70_11945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZY45451.1"
                     /translation="MSKSTDRSAALSEKLRPKVAIILQPIARGLLKLHLTPNVLTCLG
                     LLLAVGVGIAAMNGQMLLAGILMLLSGPMDAIDGAVARQSGRQTSFGAFFDSTCDRYA
                     EGFVLLGLSIYGLAINDQRIVLLAFVAVWGSLLISYTRARAEGLGIECKVGLFTRLER
                     VILISAMLILNQVLIGLIVLAILTHVTAMERIVHVWRATREL"
     gene            complement(4541..5635)
                     /locus_tag="VFF70_11950"
     CDS             complement(4541..5635)
                     /locus_tag="VFF70_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZY45452.1"
                     /translation="MAKTVGKKSKKVRVAIIGVGNCASSLVQGVEYYKDAPDGTQIPG
                     LMHTTLGGYHIGDIEFSAAIDIDVEKVGKDLNEAIFSGQNNTYKFANVPKTGIKVARG
                     MTHDGLGKYLSQVITKAPGPTDDIVKLLKDTKTDVVVSYLPVGSEEATKWYVEQILKA
                     GCAFVNCIPVFIAREKYWQQRFEDAGLPVIGDDIKSQVGATITHRILTRLFEDRGVRL
                     DRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSQLTTHQLAPNDVHVGPSDYVPW
                     LTDRKWCYIRMEGTTFGDVPLNLELKLEVWDSPNSAGVVIDAVRCAKIGLDRGMKGAL
                     TAPASYFMKSPPVQYTDDEAHAAVEKFIKG"
     gene            5812..5886
                     /locus_tag="VFF70_11955"
     tRNA            5812..5886
                     /locus_tag="VFF70_11955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5847..5849,aa:Pro,seq:cgg)
     gene            5976..7820
                     /gene="typA"
                     /locus_tag="VFF70_11960"
     CDS             5976..7820
                     /gene="typA"
                     /locus_tag="VFF70_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZY45453.1"
                     /translation="MSTLRDNIRNIAIIAHVDHGKTTLVDGMLKQSHIFRENQVVAER
                     VMDSNDLERERGITILAKNTAITYHTTKINIVDTPGHADFGGEVERVMNMVDGVLLLV
                     DAVDGPMPQTRFVLRKALEMGHKAIVVINKVDRPNARLNHVLNATFDLFIDLGASDDQ
                     AEFPTIYTNAILGHATLLPGAEGTDLQPLFDTIVSYIPAPTIEPDAPLQMLVSTLAYD
                     DYKGRIAIGRIVAGKIRKTQEVKRIAIDGAITSGKIAQVFVHQGLDRIEVDEADAGEI
                     VAITGLPEIFIGETIADPIEPKALPPIRVEEPTVRMSFGVNTSPFGGKEGTWSTSRKL
                     RERLFKELEANVSLRVEETDQADVFIVSGRGELHLAILIETMRREGYEFEVAKPEVIF
                     HEKDGVKLEPFEESYVEVAEAYLGAVVEMLGQRRGLMTDMQHGDDGTVYLKYTVPTRG
                     LLGFRSSFLTSTRGTGVIHSLFAGYQPLAGEITTRDHGSLVSWEAGLTNTFGLKNAEE
                     RGTLFVGAGVDVYEGMVIGQHIRDTDLSVNACKKKHLTNMRASTSDISVRLSTPRNLS
                     LDDCIEYLGVDELLEVTPKNLRLRKRILDTEERRKITRREGKEEVAEA"
     gene            complement(8030..>8631)
                     /locus_tag="VFF70_11965"
     CDS             complement(8030..>8631)
                     /locus_tag="VFF70_11965"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZY45454.1"
                     /translation="RKIVHVGAISIGIFHHNGQWFAHRNSCLHRGGPVCTGTLDGDIL
                     TCPWHGFQYSVVSGQLLVDPAAKLETFEVKIMDGDVHLLVPDFAESKPAPGVAELNPN
                     AFQLSDVAPGQSYLVHLDGQGVAVYNLDGAFYATQAECTHQGGPLNDGKLDGETVTCP
                     LHGSCFNIKTGAVIRGPAHRPIQTFSVVIDGAIGRVEVR"
BASE COUNT         2168 a         2405 c         2244 g         1814 t
ORIGIN      
        1 gaatagcagc gcgtggctca atgcggtgct tcgtggggga aaccaatcag attgagatcg
       61 gtgaacggac gaacgtgcaa gacggcgcga ttattcacgt cgatacgtgc cgcccgtacc
      121 tgatcagttc cagcgtcacg attgggcacg cttcggaggt gcatcgcgca gtcgtcgatg
      181 atgatgtgct gatcggcatt ggggcaaaaa ttttgagcgg cgcaagaatc gggcgcgaat
      241 gtattctggg cgcgggcaca ttgatcaccg gcagaactgt tcttctgccg atgtctttag
      301 tgattgggtt gccaggtcgc ttggtttgat cgctgaccga ggaagagatt gaatcaatcg
      361 aggcgacggc ggaaagatac gtcagatata gcgaggaata tcgcgcttcg agttaagttt
      421 gagcagcgga tcaaactaaa acaaatactc agcgaaatcc cgagaaattt cgatcgggcc
      481 agcgaattcc gatcgggctt cagcaaccag cgccgcttga tcggcattga ctgggtagtg
      541 tatcaagatc agtttttcga catgcgcctc acgcgcgagc gcacctgcca tggccgcgct
      601 ggaatgtcca aaggtggccc ccgcagtttc gtgcagcaat aagttgacgc cgagcgctaa
      661 ctgttttatc ggatcggcgg gcgcagtgtc gctggtgtac atgatggcgt aaccggtcga
      721 tcgattcttc acacgcaggc caaccaccgg cacaaagtga acgccgggtg cagccgtgat
      781 ggtgaattct tcattctcca acaccgccgc gccgatcgtg tcgggtatcg ccttgaactc
      841 caccggataa aagcgcggcc agtccgccca agcatgagcc tgcatgatcg ctttcattgt
      901 cacgatcgta gaggctggtg cataaatttt cagcggagtg gatcggccga gcagccacaa
      961 atccatcaac aggataggca gcccgtaagt gtgatcgggg tgatcgtgcg tgaggataat
     1021 tgtgttgagg gtgctgagag aaacgccggc acgctgcaag cgcccgaggg ggttgccgca
     1081 acaatcgatc aatatcgatt cacgcgcgcc tttcaacacc aggtaagtgt tttcgtgtaa
     1141 ggcgtcgtgg accgcggcag cagttcccag gatgacgagg ctcgacatgc agactctttc
     1201 taaacttgaa tgcccaaatt cacggagcta ctgacagcta tagatccagc agacgatctt
     1261 cggcgttccg tacacttcca ttaacgccgc caacaccagc agcgggaaca ccacaaacac
     1321 aaataattta atgaagtcca tcagcgccag caaccaacct tcaccgatcg ttaacttcgg
     1381 cgtgcgatga accaaggctg cgcccaaacg cagcccggcc gctgtcgcca atatcgcggc
     1441 cggcaactcg accacgccat gcggcacaaa gtacgccagg atgaaagtca gcggactgag
     1501 accggccatg ccgaccaccg gggcgatcaa ggcgatgggc acgactgcca ccatcaacat
     1561 cacgacgccg atcgagccaa acgaaaacat actcaaccca ccgccgatca gtagcgatcg
     1621 aatattattc cagaagacgg ccagcgccgg attgttggcc gcgtcgggca gtatccgtgc
     1681 aaaggcactg gcatcgaagg catggccacc gagctgcgta aagccctctc gaacttgatc
     1741 gagcatatcg tccggcaagt gcaaccggct gacaaatatc cagcccgcta ttccactcac
     1801 gatcaaaacc acgatcaccg aaaagaccgc actgcgcact cgccgataag cggctggcac
     1861 gtggacgtga tacatgttct tgatcgaaaa tcgctgtgcg gcttcacctg gattggcggt
     1921 caaccagaat aatttgaact tgcggccaat cccgatcggg tggatttgat caatctcgcg
     1981 gcccaacagt tcttcgcgat tgaacagact aaggcccatg cgcaccaaaa tcagatcgac
     2041 gacgatcagc gcaaggacga cgccccacag caccccgtaa tcggccagga acatgatcac
     2101 actctcggct tgaatgagca gcgccatggg aatgataatg aaggatgcca gcaaattcgc
     2161 agcgcgcaca ctggtggcct ggctggagac gacaaccgcg ccggacacca taacgatcgc
     2221 ttcacacgtg gtgaggatca agacttgaag caataagatt ggcggcggag accagccgcg
     2281 aaaaaccagc agaccgatca gatacacggt gatccccaag tatgcagcca acagcggtgg
     2341 aatcattgag gccagcatct tgcccagata aagctgcccg ttcgatagag gtgtcgccag
     2401 caaaggctct aagctcttcc gctcccgctc gccgacgaat gtttccaagg cgatgaccaa
     2461 tgagaacgaa atagggaaga agccaacgat catgagcagg aaggggaata agcgctctgc
     2521 aatcagcggc gtaccatagc ggaccacaaa gttctgagcg ataccggcgg tcaacgtcat
     2581 gatgatcgga aagaccagcg tcaacaaaac aatcggcacc accatccgcc aatctcggaa
     2641 agtgtcgcgc agttcgcgcc gcatcaagat cgatacgccg ccccaccaac tgagttttgt
     2701 tcgagtcgag gttatcgcgg ccatcagtat ccttccgcca tgatcggctg ctcgacaaca
     2761 cgcaaataaa cttcttccaa actgcgcgga acttcactca aggtcacaac gttgacgcgc
     2821 tgatcggcca gccagcgcag cagcggcgga ttatcgctga tcggattggc agcccaatag
     2881 cgcagccaat tcgcgccatt gctttccaca tccaagcgat cgtgcagcga aggcagcaaa
     2941 ccattcatcg cttcggcaaa gcggatttcc attaagggcc ggcctaacaa tcgggttttt
     3001 agctcgaccg gagaaccttc gatgacaatt tgtccaagtc gaatgatcgc aatatgatcg
     3061 gccatcgatt ccgcttcgac gaggttgtgc gtgcagagga tgatactgcg ccgatcgccg
     3121 cgcaaggttg acagcgcgtc acgcaccaag cgcgcacttt gaggatccat tgccgaagtt
     3181 ggctcatcca gcagcaggac atccggatcg tgcagcatac agcgaatcaa tgccagcttt
     3241 tgtttcatcc ctttggagaa ttcgctcaag cgccgattca gcacatccga catttcaaac
     3301 tgcgtcatca gcgttttgat ccgctcgcgc gtttgcgccg tcgacaaacc atagacgctg
     3361 ccaaagaatt ccaaatattc gcgcgcggtc gatcgtaaat acaagccggg catctcggtg
     3421 agaatgccca cgcgctgtcg aacttcttcg gcgttcttcc gaatatctag cccagcaatt
     3481 ttcgcccagc cggcggtcgg caccaagata ccggacagca tgcgcacagt cgtcgtttta
     3541 cccgcgccgt tcgggccaag cagcgctaac acttcgcccg ccgcaacgcg caaagaaaca
     3601 tcgcgtacag ctgcaaagtc accgaaagat ttgctcaagc cttgagcttc aatcacgtta
     3661 actcactttt taggggaatg gctgtcaaga ttatagcagg tttaattcat gtttcaatgc
     3721 agttcaagta ctaattcaaa tcaccgcaaa ctctctaaaa accgagcaag aattaggtat
     3781 tgcgatcgca gttagaacct tcggcagact tttaccaaca aaacaccaac aactgcgcag
     3841 gctgtgaagt ttcaaagttc acgcgtggcg cgccaaacat gcacaatgcg ctccatggcc
     3901 gtaacatgcg ttaagatcgc aaggacaatc agcccgatca aaacttgatt aagaattaac
     3961 atggcgctga tcagaataac gcgctccagg cgcgtgaaca ggccaacctt acattcaata
     4021 cccagtcctt ccgcacgcgc gcgggtataa ctaatcaaca gtgatcccca gacagcgacg
     4081 aatgctaaca gcacgatacg ttgatcgtta attgctaagc cgtagatcga taggcccagc
     4141 agcacgaagc cttccgcata tcgatcgcag gttgaatcga agaaagcgcc aaagcttgtc
     4201 tgtctgcccg attgtctagc gaccgcccca tcgatcgcat ccatcggacc ggacagcagc
     4261 atgagaattc cggctaacaa catctggcca ttcattgccg caatgccaac gccaaccgct
     4321 aagagcaggc ctaaacacgt gaggacattc ggcgtgagat gcagcttcaa cagaccacgc
     4381 gcgattggtt gcaagatgat cgccactttg ggacgtagtt tttcactcaa cgccgcggat
     4441 cgatcagtcg atttgctcaa tagataccct catcagtcag ataacaatag cacggccatc
     4501 gatccgagat cgatggccgt gctataatat tcaaaaccac ttaacctttg atgaatttct
     4561 caaccgcagc atgcgcttca tcatcagtgt actgcaccgg cggcgacttc ataaagtatg
     4621 aagccggcgc tgtgagcgcg cccttcatac cccgatcgag tccgatcttc gcgcagcgga
     4681 ctgcatcaat caccacgccc gctgaattgg ggctgtccca cacttccaat ttcaattcaa
     4741 gattcagcgg cacatcgccg aaggtcgtgc cttccatccg gatgtagcac cacttgcgat
     4801 cagtcaacca cggcacatag tcactggggc cgacatgcac atcgttcggc gccaattgat
     4861 gggtcgtcaa ttgactggtg accgcgttcg tcttgctgat cttctttgac tccaggcgtt
     4921 cgcgctccaa catgttcagg aagtccgtgt tgccgccgaa gttgagctga tacgttcgat
     4981 cgagccgcac gccgcgatcc tcaaacagac gcgtcaagat gcgatgcgta atcgtcgcac
     5041 cgacctggct cttgatgtca tcgccgatca ccggcagtcc ggcatcttca aagcgctgct
     5101 gccagtattt ctcgcgggcg ataaaaaccg gaatacagtt gacgaaggcg cagccggctt
     5161 tcaatatttg ttccacatac cacttcgtgg cttcctcact gcccaccggt aaatacgaca
     5221 cgacaacatc ggtcttggtg tctttgagca gctttacaat atcatcggtc ggaccgggcg
     5281 ccttcgtaat aacttgcgac agatatttgc cgagtccatc gtgcgtcatg ccgcgcgcca
     5341 ctttgatacc ggtcttcggc acattggcga atttataagt gttgttctga ccggagaaga
     5401 tcgcttcgtt cagatccttg cctacttttt cgacgtcaat atcgatggcg gcactgaatt
     5461 caatatcgcc gatatggtag ccacccagag tcgtatgcat gagtccgggg atttgagtgc
     5521 catctggcgc atctttataa tattcaacac cctgcaccaa cgacgaagcg cagttgccca
     5581 cgccgataat agctacacgg actttcttgg acttcttgcc gactgttttt gccacaggtc
     5641 attctccttg attgaactaa gttaccactt aattgtacac catcgcggcg ataaagttac
     5701 atcgattgac aagttctgtc aactcgaatg aggatcgatt aacgtctgcg cgaaaaattt
     5761 tcttcgattg tcttgcggat tggatcgaac tcaggtataa tcgtgaacgt tcggggcgta
     5821 gcgcagtccg gttagcgtgc acggttcggg accgtgaggt cggaggttca aatcctctcg
     5881 ccccgactaa tctccacaac aaattgtgga ggtttttatt tgaagggtca catatcatgc
     5941 agcctcttag taagaaagtg tagtaaaagg attacatgtc aacattacgc gacaacattc
     6001 gaaacatcgc catcatcgcc cacgtcgatc atggcaagac aactttggtc gatggcatgc
     6061 tgaaacagag ccacatcttc cgcgagaatc aagtggtggc cgagcgtgtc atggacagca
     6121 atgatcttga gcgcgagcgc ggtatcacga ttttggccaa gaacacagcc atcacttatc
     6181 acaccaccaa gatcaacatt gtcgatacac ccggccacgc cgatttcggc ggtgaggtcg
     6241 agcgtgtgat gaacatggtc gatggcgtgc tgctgctggt cgatgccgtc gatgggccaa
     6301 tgccacaaac gcgcttcgtt ttacgcaagg ctctggaaat gggtcacaaa gcgatcgtgg
     6361 tcatcaacaa agtcgatcgg ccgaatgccc gcttgaatca cgtgttgaac gcgaccttcg
     6421 atctctttat cgatctcggc gcatccgacg atcaagccga attcccgacg atctatacta
     6481 atgcgatctt gggccacgca acgcttttgc cgggcgcaga aggcacagac ctgcagccac
     6541 tattcgatac catcgtttct tatattccgg caccgacgat cgaacctgat gcgccgctcc
     6601 aaatgctggt cagcacgtta gcttacgatg attacaaagg ccgcatcgcg atcgggcgca
     6661 tcgtcgctgg aaaaattcgc aaaacacagg aagtcaaacg cattgccatc gatggcgcga
     6721 tcacatccgg taagatcgcg caagtcttcg tgcatcaagg tctggatcga attgaagtgg
     6781 acgaagcaga tgcaggcgag atcgtagcga tcaccggcct gcccgaaatc ttcatcggcg
     6841 aaacgatcgc tgatccgatc gagccgaagg cactgccacc gattcgcgtt gaagagccga
     6901 cggtgcgcat gagtttcggc gtcaacacca gtccgttcgg cggcaaggaa ggcacctgga
     6961 gcacgtcgcg caaactacgc gagcgattat tcaaggaact cgaagcgaat gtctcgctgc
     7021 gcgtggaaga aaccgatcag gccgacgtct ttatcgtgtc cgggcgcggg gaactgcatc
     7081 tggcgatctt gatcgaaacc atgcggcgcg aaggctacga attcgaagtc gccaagccgg
     7141 aagtcatctt ccacgaaaaa gacggcgtga aacttgaacc gtttgaagaa agttacgtcg
     7201 aagtggccga agcgtatctg ggcgcggtcg tcgagatgct gggtcagcgg cgcggcctga
     7261 tgaccgatat gcagcacggc gatgatggca cggtctatct taaatatacg gtgcccaccc
     7321 gcggactgct cggcttccga tcgagctttc tcacttcgac ccgcggtacg ggtgtcattc
     7381 attcgctctt cgccggatat cagcccttag caggcgaaat caccacgcgc gatcatggct
     7441 cgctggtcag ttgggaagcg ggtctgacta acactttcgg actaaagaat gccgaggaac
     7501 gcggcacatt attcgtcggc gcgggtgtcg atgtctatga aggtatggtc attggtcaac
     7561 acattcgaga cacggatctg tccgtgaacg cctgtaagaa aaagcatttg accaacatgc
     7621 gcgcttccac gagcgacatc tcggtacgct tgtccacgcc gcgcaacttg agtttggacg
     7681 actgtattga atatctgggt gtggatgaat tactggaagt cacacccaaa aatttgcggc
     7741 tgcgcaaacg catcctcgac acggaggaac gtcgcaagat tacccggcgc gaaggcaagg
     7801 aagaggtcgc ggaagcgtaa tcaagatcag tctatcaata gactaagcat ccaaagcctg
     7861 caacacaaga aacgagccgg gattcgattg agtctcggct cgatatctta acgaagtttt
     7921 tacacagttg tcgtgatcgg ttatacctct actccaatgc ccactgctac gacgatacta
     7981 ttttgtgcgg ccgacaatgt cagatcgttc tcgatcgatc ttctacgctt caacgaactt
     8041 ctactcgccc aatcgcacca tcgataacaa cactgaatgt ctggatcggc cggtgagcgg
     8101 gaccgcgaat aaccgcgccg gtcttgatgt tgaagcatga gccgtgcagc ggacaggtca
     8161 cagtttcgcc gtcgagtttg ccgtcattca gtgggccgcc ttgatgcgtg cattcggctt
     8221 gcgtcgcgta gaatgcccca tcgagattgt aaacggccac gccctgcccg tcgagatgaa
     8281 cgagataaga ttggccggga gcaacatcgg acagctggaa tgcattgggg ttaagttcag
     8341 caactccggg tgcaggtttt gattcggcaa aatccggcac gagcaaatga acatctccat
     8401 ccataatctt gacttcgaac gtctccaatt tcgcggccgg atcgacgagc agctgcccgc
     8461 taacgacgct atactgaaag ccatgccacg gacaggtcaa gatatcaccg tcgagcgtac
     8521 cggtgcacac cgggccaccg cgatgcagac acgaattccg atgcgcgaac cactggccgt
     8581 tgtgatggaa gatgccgatc gagatggcac cgacgtgcac aatcttgcga c
//
LOCUS       DASRTJ010000587         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_264399,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000587 DASRTJ010000000
VERSION     DASRTJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264399"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..418
                     /gene="gcvH"
                     /locus_tag="VFF70_11970"
     CDS             32..418
                     /gene="gcvH"
                     /locus_tag="VFF70_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZY45455.1"
                     /translation="MNFPTDVKYTKTDEWIRVKGNEAEVGLTDYAQNHLSDIVYVELP
                     EVGAEFKQATAFGSIESVKAASDLNLPVSGKISAVNDSLADTPEVVNSDPFGKGWIVK
                     CSVANAAELSGLMDAAAYQKYCEERG"
     gene            492..1838
                     /gene="gcvPA"
                     /locus_tag="VFF70_11975"
     CDS             492..1838
                     /gene="gcvPA"
                     /locus_tag="VFF70_11975"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZY45456.1"
                     /translation="MTYIPHTDANRAEMLKAIGVKSLKDLFPDVPAQHKYPKLTVPAA
                     LSEMETWWEMQGLSEANADVNHYACFLGAGAYNHYSPHTVDYVLRRGEFYTAYTPYQP
                     EVSQGTLQAMFEYQSMICQLTGMDVSNASHYDGATSLAEGVLMSMNTTKGNRNKIVMS
                     PLVHPQYRAVVRTYTQGMDVSIVGDDNLKSDLPDLIKLLDDRTAALVIQDPNFLGQLE
                     DINGLADKVHAVGALLIVVTDPIALGLFTPPGEYGADIVCAEGQSLGIPLSYGGPYLG
                     IFATKNAYVRKMAGRLVGETVDSDGTRGYVLTLSTREQHIRREKATSNICTNQGLYML
                     AAAVYMATLGKCGLEQVARLCWHKSHYAAKAIGKLKGYKVITDKPFFKEFVVKLPKPV
                     SEVNEFLLEEFDILGGYDLGQDYPHLKNHMLIAVTETNTQQEIDDLVEGLKQVMEQ"
     gene            1841..>2034
                     /locus_tag="VFF70_11980"
     CDS             1841..>2034
                     /locus_tag="VFF70_11980"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZY45457.1"
                     /translation="MSEPLIYEISSPGRCGVEMPASDVPETDLPKDLLRSNLGLPELS
                     QLDVVRHYMHLSQMNYNIDGG"
BASE COUNT          511 a          542 c          560 g          421 t
ORIGIN      
        1 cacaaaataa ttttttagcg aggagcgaac catgaatttc ccaacagatg tgaagtacac
       61 caagaccgat gagtggatcc gcgtcaaagg caatgaggcg gaagtcggcc tgaccgatta
      121 tgctcagaat catctgagcg atatcgtcta cgtggaactg cccgaggtcg gcgcggagtt
      181 caagcaggcc actgctttcg gatcgatcga atcggtgaaa gccgcgtccg atctgaatct
      241 gccggtcagc ggcaagatca gcgcggtgaa tgattcattg gccgacacgc ccgaagtggt
      301 caacagcgat ccgtttggca aaggctggat cgtgaagtgc tcggtggcca atgccgccga
      361 actcagcggc ttgatggatg cagcggcgta tcaaaagtat tgcgaagaga gaggctagtt
      421 agtaattagt gattagtatt gaggacgcac ttacttttta cgatttacta atcatagaaa
      481 ggtaaagatc aatgacttac attcctcaca ccgacgccaa tcgcgcagag atgttgaaag
      541 cgatcggcgt caaatcactc aaagatttat tccccgatgt cccggctcag cacaagtacc
      601 cgaagttaac cgtccccgcg gcgttatccg aaatggaaac gtggtgggag atgcaaggtt
      661 tgtccgaagc caatgccgat gtaaatcatt acgcgtgttt cctcggtgcg ggcgcgtaca
      721 atcattacag cccgcacact gtcgattacg tgctgcggcg cggcgagttc tacaccgcct
      781 atacgcccta tcaacccgaa gtaagccagg gcaccttgca agcaatgttc gaatatcaaa
      841 gcatgatctg ccaacttacc ggcatggacg tcagcaacgc cagccactac gacggcgcga
      901 cttcattggc cgaaggcgta ttgatgtcga tgaatacgac caagggcaat cgcaacaaaa
      961 ttgtgatgtc gccgctggtt catccgcaat atcgagcggt ggtgcgcact tacacgcagg
     1021 gcatggacgt atcgattgtt ggcgacgaca atttgaaaag tgatttgccc gatctgatca
     1081 agctgctcga cgatcgaacg gccgcgctgg taatccagga tccgaacttc ctcggccagc
     1141 tcgaagatat caatggcctg gccgataaag tccacgcggt cggtgcatta ttgattgtgg
     1201 tcactgaccc gatcgcgctg gggctgttca ccccgcccgg cgaatatggc gcggatatcg
     1261 tctgcgccga aggacaatcg ctgggcatcc cgctgtcgta tggcggaccg tatcttggca
     1321 tcttcgcgac gaagaacgcc tacgtgcgca agatggcggg acgcttggtc ggcgaaacgg
     1381 tcgatagtga cggcacacgc ggttacgtgc tgacgctttc gacgcgcgag cagcacatcc
     1441 gccgcgagaa agccacttcg aatatctgca ccaatcaagg cttgtatatg ttagccgcgg
     1501 cagtgtatat ggcgaccctc ggtaaatgcg gcctggaaca agtggcgcgc ttgtgctggc
     1561 acaagagtca ttacgctgcc aaagcgatcg gcaaactcaa aggctacaaa gtcatcaccg
     1621 acaagccgtt tttcaaagag tttgtggtga agctgcccaa gccggtcagt gaagtgaacg
     1681 aattcctgct ggaagaattc gacatcctcg gcggctacga tctgggtcaa gactatccgc
     1741 acttgaagaa ccacatgctg atcgccgtga ccgaaacgaa cacccagcag gagatcgacg
     1801 atctggtcga aggtctcaag caggtaatgg agcaataacc atgagcgaac ctcttatcta
     1861 cgaaatcagt tcacccggcc gctgcggcgt tgagatgccc gcgtccgatg tgcccgaaac
     1921 cgatctgccc aaagacttgc tgcgatcgaa cctcggcctg cccgaacttt cgcagctcga
     1981 tgtggtgcgg cactatatgc acctgtcgca aatgaactac aacatcgacg gcgg
//
LOCUS       DASRTJ010000588         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_36963,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000588 DASRTJ010000000
VERSION     DASRTJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36963"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="ricT"
                     /locus_tag="VFF70_11985"
     CDS             complement(<1..980)
                     /gene="ricT"
                     /locus_tag="VFF70_11985"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HZY45458.1"
                     /translation="MDAPIVEAVTSSPPIDEKKIVGVRFMPVGKVYHFDATRLADLRQ
                     GDWVLVTTSRGRQIGEVATLNPPKHGEADGPFKAIERLATNRDLALKKHWEGQEISAM
                     VFARERAKLMNVAAKITKAEYTFDGSRLTFLYTVEEEANVNIDPLLNDLQKAYRSKVE
                     LRLIGPRDAAKIIGGGGACGLTERCCTKFLTDFSPISVKMAKEQGLSLNPQEITGMCG
                     RLRCCLLYEYEQYVDARKLLPKRNKEVMTPSGRGKVIEVLPLRDSVLVQVAEMRIEVK
                     RADLQPLAELEAFQKKIDQPCSGQHGCSCGASKRTGKNRDNKVQRDRGEQ"
     gene            complement(1010..2383)
                     /locus_tag="VFF70_11990"
     CDS             complement(1010..2383)
                     /locus_tag="VFF70_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZY45459.1"
                     /translation="MSETNLISDPIFTPISGPVFTTPELPTYESLMDCIRCGRCLPVC
                     PTYQQTTLETQSPRGRLSLLRAVEDGKLDLDDPGVEEHLYHCLDCRACNVVCPAGISI
                     GECIVEGRAAVEEKHPRSWIIRFVLNHALISPRRIEWFAWPLRLAQQLKLDKLLLFLF
                     GWIPAIRDLMQMAPAMAKPLWKELTNPPAGFSSTRMDVQSNLREVGQYRVGFFLGCVM
                     NVAYGDASRASVKLLKQFSCEVVTPKAQVCCGAPQDDQGLKDQARRMAKRNIEAFEKL
                     GELDAIVADCAACSGYLKEYTHVLHNDPEWAAKADWFSAKVKDITEWLDQIMPQDIAT
                     TIAGSRSITYHEPCHLANVQGVRRQPRALLARLKNKGADVRELTDATRCCGSAGIYNL
                     THSTMSKELLDRKMADIAATGAETVISANPGCLLQLQWGARRTGLDVKVKHLTQVLVE
                     SLAERED"
     gene            2548..3252
                     /locus_tag="VFF70_11995"
     CDS             2548..3252
                     /locus_tag="VFF70_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45460.1"
                     /translation="MSKILIVDDEPPIIDVLSYNLKQSGYEVVIARDGEQALMLARRE
                     QPDLIILDLMLPKLDGLEVHRILRRESDTPVIMLTARDGEIDRVVGLELGADDYVVKP
                     FSVRELIARVKNLLRRSIPKPITNLPDRIEVGELSIDVTRHEACFGSIRLELTALEFA
                     MLHTLLRHVDQVLSREQLLEQVWGYDYHDDLRVVDAVIKRLRSKLRQASPKADVIATV
                     RSIGYKLISKPESLEA"
     gene            3249..>4728
                     /locus_tag="VFF70_12000"
     CDS             3249..>4728
                     /locus_tag="VFF70_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45461.1"
                     /translation="MRLFSSLRSRLVFSHLTVIVLAMGLSGLLLLTFLQDYFVRSTED
                     SLIAQARLTVQALVPDANAVGPSITTQAPLNNALQQSASNLVVQATQQTSPSLNTADL
                     NYLANTSLQLSAQIDTRIRLLNADGVVLVDTAKTDTGLNLHDDPLVRRALQGQYASSS
                     DLTSDQARVVVTMPVLVDGKTIGAAYLSESLRDVLTVLQDLRVRLVLATAIALILSGT
                     MSWWLSRAITRPVRQLTAAADAVAVGRFDQWVSVQSHDELGRLGRTFNDMTTRLQSAR
                     KMQIDFVADVSHELRTPLTSIKGTIETLRSGAVDDLGVRDQFLETVEAETDRLIRLVS
                     DLLVLSRADSAALNLRRESIDLGQLIRAVTNRLADQAAMHQIKLQVSIDPDMPLVSAD
                     ADRIEQVLLNLLDNALKYSPLDRTVTIHLAAETNRSVMVQVRDEGRGISAADLDRIGQ
                     RFYRADKSRARGGHGLGLAIAQSLIQAHGGRLWLESKEGAGTT"
BASE COUNT         1033 a         1363 c         1240 g         1092 t
ORIGIN      
        1 ttttgctcgc cacgatcgcg ttgaactttg ttgtctctgt ttttgcccgt gcgtttactc
       61 gcgccacagc tgcagccatg ctgcccgctg cagggttgat cgattttctt ttggaacgct
      121 tcgagttcgg ccaacggctg caggtcagcg cgcttgactt cgatgcgcat ctcggcaact
      181 tgcactaaca ccgagtcacg cagcggcaac acctcgatca ctttaccgcg cccgctcggc
      241 gtcatcactt ctttgttgcg tttgggcagc agcttgcgcg catccacata ctgctcgtac
      301 tcatacaaca aacagcaccg caggcggccg cacatgccgg tgatctcttg cggattcaac
      361 gacagcccct gctctttcgc catcttcacc gagatcggac tgaagtcggt gaggaacttg
      421 gtgcaacagc gctcggtcaa gccgcacgcg ccgccgccgc cgatgatctt cgccgcatcg
      481 cgcggaccga tcaagcgcag ctccactttc gatcgatacg ctttttgcaa atcgttcagc
      541 agcggatcga tgttgacgtt ggcttcttct tcgaccgtat acaaaaacgt caagcgcgat
      601 ccatcgaacg tgtattcggc tttggtgatc ttggcggcca cattcataag ttttgcccgc
      661 tcacgcgcaa acaccatcgc gctgatctcc tgcccttccc aatgtttctt gagcgcaaga
      721 tcgcggttgg tcgccagccg ctcgatcgct ttgaatggcc catcggcctc accgtgcttc
      781 ggcggattga gcgtggccac ttcgccgatt tgccggccgc ggctggtggt caccaagacc
      841 caatcgcctt gacggagatc ggctaagcgc gtcgcatcga aatgataaac cttgcccacc
      901 ggcataaatc gcacgccgac gattttcttt tcatcgatcg gtggtgatga agtgactgct
      961 tctacaattg gtgcatccat acttcccccg tgctgtaatc cgagtgtggt caatcttcac
     1021 gctcggctag cgattcaact aagacttgag tgagatgttt tactttgaca tcgagaccgg
     1081 ttcgtctcgc tccccactgc aattgtaaca aacaaccggg atttgcacta atcaccgttt
     1141 cagcgccggt cgctgcaatg tcggccatct tgcgatcgag cagttctttc gacatcgtcg
     1201 agtgtgtgag attgtaaata ccagcactgc cgcaacaccg cgtcgcatcg gttaattcgc
     1261 gcacatctgc gcctttattt ttcaagcgcg ctaataacgc acgcggttgg cgtcgaacgc
     1321 cctgcacatt cgccaaatga cacggctcat gatacgtgat cgatctcgat ccagcgattg
     1381 ttgtagcgat gtcctgtggc atgatctgat cgagccactc agtgatatct ttgaccttcg
     1441 ctgaaaacca atcggccttt gctgcccatt cgggatcgtt gtgcagcaca tgcgtatatt
     1501 ctttcaaata cccgctgcac gccgcgcaat cagcgacgat cgcatccagt tcccccaatt
     1561 tttcaaaagc ctcgatgtta cgcttcgcca ttcggcgcgc ttgatctttg agaccttgat
     1621 cgtcttgggg cgcgccgcag cacacttgag ctttgggcgt aaccacttcg cagctaaatt
     1681 gtttgagcaa ctttactgaa gcgcggctcg catcaccata ggcgacattc atgacacagc
     1741 ctaagaagaa accgactcga tattggccaa cctcccgcag gtttgattgc acgtccattc
     1801 gagttgaact aaaacctgcg ggaggattag ttagttcttt ccacaacggc ttcgccatcg
     1861 ctggcgccat ctgcatcaga tcgcgaatcg ccggaatcca gccgaaaaga aacagcagca
     1921 acttatctaa cttcaattgt tgagccaatc tcaacggcca cgcgaaccac tcgatccggc
     1981 gcggtgagat cagcgcatga ttcaacacga agcgaatgat ccacgatcgc ggatgctttt
     2041 cttcaaccgc cgcgcgtccc tcaacgatgc attcgccgat cgagatacca gcaggacaaa
     2101 ccacattgca ggcgcggcaa tcgaggcagt gatagagatg ctcttcaacc ccgggatcat
     2161 ccagatcgag tttgccgtct tcgactgcac gcagcaacga caatcggcca cgcggtgatt
     2221 gcgtttcgag cgtcgtttgt tgataggtgg gacacacggg cagacagcga ccgcagcgaa
     2281 tgcaatccat gagtgattca taggtcggca attcgggcgt ggtgaagacg gggccggaga
     2341 ttggggtgaa gatcggatcg gatatcaaat tagtttcgct catcaactat aaccaataat
     2401 cgacgttgtg acagtatctt accatagcga ttacccgcct gcaatcgatc tcaatttaac
     2461 gcaagtttgt catcgatttg tcacacaacc catttactca gcagccgctt tcggtataca
     2521 atcaagccag caaagagagt gctgcggatg agcaaaatat tgatcgtcga tgacgagccg
     2581 ccaattatcg acgtcctgtc ttacaatctc aaacagtcag gctacgaggt cgtcatcgcg
     2641 cgcgacggcg aacaagcctt gatgttggcg cgccgcgagc agcccgattt gatcatcctc
     2701 gatctgatgc tgcccaaact cgacgggctg gaagtgcatc gcatactgcg ccgcgagagt
     2761 gacacgcctg tgatcatgct cacggcacgc gacggcgaga tcgatcgcgt ggtgggcttg
     2821 gaattgggcg ctgacgatta cgtcgtcaag cccttcagtg tgcgcgaatt gatcgcgcgc
     2881 gtgaaaaacc tgctgcgccg atcgatcccc aagccgatca cgaatttacc cgatcgcatc
     2941 gaggtcggcg aactctcgat cgatgtcacg cggcatgaag cgtgtttcgg ctcaattcga
     3001 ctggaactca cagctttgga attcgccatg ctgcataccc tgttgcgcca cgtcgatcaa
     3061 gtcttgagcc gtgaacaact actcgagcaa gtgtggggct acgattatca cgacgatttg
     3121 cgcgtggtcg atgcggtgat caagcgtctg cgatcaaaat tgcggcaggc gtcgcccaaa
     3181 gccgatgtga tcgccaccgt ccgcagcatc ggctataaac taatctcgaa gccggagtcg
     3241 ttggaagcat gagactattt tcgagtctgc ggtcgcgttt ggtcttcagc cacttgacgg
     3301 tgatcgttct agccatgggc ttatccggtt tactgctgct gaccttcttg caagattatt
     3361 tcgtacgctc gaccgaggat agtctcatcg cgcaagcacg cttaacggtg caagcccttg
     3421 tgcctgatgc aaacgctgtc gggccgtcga tcacgaccca agcgccgctc aacaatgccc
     3481 ttcaacaatc agccagcaat ctggtcgtgc aagccactca acagacgtcg ccatcactta
     3541 atacggcgga cctcaattac ttagcgaaca cttccttgca actcagcgcg cagatcgata
     3601 cgcgcattcg attgttgaac gccgacggcg tcgtgttggt cgataccgcg aagacggata
     3661 ccggtctcaa tctgcatgac gatccgctcg tcagacgtgc actgcaaggc caatatgcca
     3721 gcagcagcga tctcacttcc gatcaagccc gcgtggtggt cacgatgccc gtcctggtcg
     3781 acggcaaaac gatcggcgcc gcttatctga gcgagagcct gcgtgatgtg ttaaccgtgc
     3841 tgcaagattt gcgagtcaga ttagttctag caacagctat cgccttgatc ttatcgggca
     3901 cgatgagttg gtggctgtcc cgcgcgatta ctcgaccggt acgccaactg acagcggccg
     3961 cggacgctgt ggcggtgggg cgcttcgatc aatgggtgtc ggtccaatcg cacgacgaac
     4021 tcggacgatt gggccgcact ttcaacgaca tgacgacgcg gctgcaatct gcgcggaaaa
     4081 tgcagatcga ttttgtggca gatgtctcac atgaattacg cacgccactc acttcgatca
     4141 aagggacgat cgaaacactg cggtcgggcg cagttgatga tctgggtgtg cgcgatcaat
     4201 ttttagagac ggttgaagct gaaaccgatc ggttgatccg gcttgtcagc gatctgctcg
     4261 tgctgtcccg cgccgattcc gcggcgctca acttgcggcg tgaatcgatc gatcttggac
     4321 aactgattcg cgctgtgacc aatcggttgg ccgatcaagc cgcaatgcat cagatcaagt
     4381 tgcaagtcag catcgacccg gacatgccgc tggtatcagc tgatgcggat cggatcgaac
     4441 aggtgctgct aaatcttttg gataacgcgc tgaagtattc acccctcgac cgcacggtga
     4501 cgatccatct agctgctgag actaatcggt ctgtcatggt ccaagtgcgt gacgaaggca
     4561 ggggcatctc agccgccgat ctcgatcgaa tcggccaacg cttctatcgc gctgataaat
     4621 cccgcgcgcg cggcggacat ggtcttggcc ttgccatcgc ccaaagtttg attcaagccc
     4681 acggcgggcg attgtggctg gagagcaaag aaggcgcggg cactacag
//
LOCUS       DASRTJ010000589         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75214,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000589 DASRTJ010000000
VERSION     DASRTJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75214"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..877
                     /locus_tag="VFF70_12005"
     CDS             371..877
                     /locus_tag="VFF70_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45462.1"
                     /translation="MTNLFEVKTIRVAKRIDLPLSPVRRRVHVLMRVIPISLTVFVLI
                     YEFGLSEWIQAHFGTVYHSFTDMAIYGIAGPVLAYLVLHFLDRWLEERETSELQAQLL
                     TQARQQAQTSAELSDTALQTLFAASILISSLKSGSTELLPETKIELARTEESLNRAIQ
                     RIRDHLES"
     gene            1006..2343
                     /locus_tag="VFF70_12010"
     CDS             1006..2343
                     /locus_tag="VFF70_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45463.1"
                     /translation="MNDKREIRGWMLYDWANSAFSTTVVTVFLGPYLTGLIQAQVGEN
                     GTFALFGITIAAKSFFTYCVSASVFLQIFFLPILGAIADYSRLKRKLMAVFAVLGAIC
                     TIGLFLINGELYVLGGLLFIIANLCFGASIVFYNAFLPQIVSEDQRDRVSSGGWAWGY
                     AGGGLLLLINLGLYNFGDQIGLTVAMVARISLASAGIWWLIFGWVAISRLRDRGAHRV
                     LPAGQSYISIGFRQLRQTLGEMRKYPMTLRYLIAYLLYNDGVQTVIVVAALFGDQELK
                     MAISDLTLVILMVQFVGIFGALGFGRLAERIGDKKTIIITLLVWSATSVWALLSLRSV
                     GEFWLLAAVVALVLGGSQALSRSLFSRMIPRGREAEFFSFYEISERGTSWIGPLLFGL
                     VTQATNSMRTAIFSLIVLFVGGLLVLITVNVPRAIHESGNDVELLLEPETAPA"
     gene            2521..3447
                     /locus_tag="VFF70_12015"
     CDS             2521..3447
                     /locus_tag="VFF70_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45464.1"
                     /translation="MSQAFAVETAHLRKEYGRKVAVEDLNLQVRPAEVFGFLGPNGAG
                     KTTSIKMLLGLAEPTKGEARLLGKPIGDPAARSRVGFLPEHFRFHDWLTGEEFLQVHG
                     QLYGMSEIDRKQRVPDLLKRVGLEAHADKRLRGYSKGMLQRVGLAQALLNHPAVVFLD
                     EPTSGLDPIGRVLVRDLIHELRDEGTTVFLNSHLLSEVEITCDRVVFIKHGSVVKDVV
                     LKELELGPMPVLIKVGALPSIEDLRRDLERWGHDISIEANHIRLIVDDDSHLPDLARW
                     LIGQGIDLYSLMPQHVSLEELFLQIVGAEESL"
     gene            3449..4282
                     /locus_tag="VFF70_12020"
     CDS             3449..4282
                     /locus_tag="VFF70_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45465.1"
                     /translation="MPIPILIRLTIREALRRKVILGLLILGAVFLILFSLGVGFAHGQ
                     ILEYGSGATGRLPISSVYSFLLTSGLYAANFLIVMLSVLVSVDTISGEISTGTIQSIA
                     VKPIRRSDILLGKWLGLMIMLGACVLFLSGGVILLTALITGYVPPNPLGAIVLMFLES
                     IVLLCASLLGGTRLSTLANGVFGFGLFGAALIGGFIEQIGAFLNNDAALTIGHIATIV
                     MPSDALWRLALTQTAEGVNPFRLMFGGLSEPDPNIVVYALVFAGIVLLLAIRSFSKRD
                     L"
     gene            complement(4349..>4609)
                     /locus_tag="VFF70_12025"
     CDS             complement(4349..>4609)
                     /locus_tag="VFF70_12025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZY45466.1"
                     /translation="AYMIGGAGSDYYNLPGVAFPVYFTYSGCATHGTYWHNDFGRPRS
                     HGCVNATNQAAEWLFRWTEPAVPYTEYGLPIKRGEGTKVVVV"
BASE COUNT          953 a         1096 c         1303 g         1257 t
ORIGIN      
        1 aggcgcgcca gcggtgaaag tcgacaaaga aaaagttgat ctgggtaatg tgaaattggg
       61 gcagacagtc tcagtttcat tcgatgtcac taacgtcggt gatcaaccat tgcgcttcag
      121 tgaagtgccg tatgttgaag tcgtggaggg atgttgaccg cctacaccgg ccatcggttc
      181 gatggtcctc atgccaggtc aacacactat agtttcaatg tcgttcatga tgcatggcga
      241 tatgggcggg cggcacgatt ttcgcgttca cttgatgaca aacgtggcat cgcaaccaga
      301 taaagaagtg acaatattgt cgaattggat ccaataaact tgatagttca ccaaatcttg
      361 cacattttca atgacgaatc tttttgaagt gaagacgatt cgagttgcga aacgcatcga
      421 tctgccgctg tcgcctgtgc gtcggcgagt gcatgtgctg atgcgtgtga ttccaatcag
      481 cttgacggtg ttcgtgctga tctatgagtt cggcttatcc gaatggattc aggctcactt
      541 tggcaccgtt tatcattcat tcaccgatat ggcgatctat ggaatcgccg ggccggtatt
      601 ggcctatctg gtgctgcatt ttctcgatcg ttggttggaa gaaagggaga caagtgaact
      661 acaagcccag ctcttaactc aagcgcgcca gcaggcgcag accagcgcgg agttgagtga
      721 tactgcgctc caaactttgt tcgccgccag cattttgatt tcatccctga agtccggcag
      781 tactgaattg ttgcccgaaa caaagatcga gctagcccgc accgaggaat cactcaaccg
      841 cgccattcaa cgaatccgcg atcacttgga aagctgatac tgcccagttg aagttcttga
      901 attgactttt tgtgtgtttg attctaaact ccccgcactc cacagtggcc gtgtcgggga
      961 gttgttgttt cttctacaag ttccaatctc aattcggagg caggcatgaa tgataagcgc
     1021 gagattcgcg gttggatgtt gtatgactgg gcaaactctg ctttttccac gacggtggtc
     1081 actgtatttc tggggccgta tctgaccggc ttgattcaag cccaagtcgg cgagaacgga
     1141 acctttgcct tgtttggcat caccattgct gccaaatctt tttttactta ctgcgtctca
     1201 gcctcggtct ttcttcaaat ttttttcctg cctatcctgg gcgcgattgc cgattactcg
     1261 cgtctcaaac gcaaattgat ggcggtattt gccgtgctcg gggcgatctg cacgatcggt
     1321 ttattcttga tcaatggcga attgtatgtg ctgggtggct tgctgtttat cattgccaac
     1381 ttgtgcttcg gcgcatcgat cgttttctac aatgcgtttc tgccgcagat tgtttctgaa
     1441 gatcagcgcg atcgagtgtc gtccggcggt tgggcttggg gttatgccgg cggtggattg
     1501 ctcctgctga ttaatctagg cctctataac tttggcgatc agatcggcct gacggtagcc
     1561 atggtcgcgc ggataagttt ggcatcagcc ggcatatggt ggctgatctt tggctgggtt
     1621 gcgatcagtc gtctacgcga tcgcggcgca catcgtgtgt tacccgcagg tcagagttat
     1681 atctcgattg gctttcgtca actccgacaa actttgggag agatgcgcaa atacccgatg
     1741 acactgcgct atctcatcgc gtatttgctc tacaacgacg gcgtccaaac agtgattgtt
     1801 gttgcggcac tgttcggcga tcaagaattg aagatggcaa tcagcgatct gaccctggtg
     1861 attttgatgg tgcagtttgt gggcatcttc ggtgcattag gctttggccg cttggctgaa
     1921 cggattggcg acaaaaagac gattatcatt acgctattgg tctggtcggc tacaagcgtg
     1981 tgggctttgc tcagcctacg cagcgtcggc gagttctggt tgttagcggc ggtggtggcg
     2041 ttggtgctgg gtggcagtca ggccctgagc cgatcgcttt tctcgcgcat gatcccgcgc
     2101 ggtcgcgagg ccgagttctt tagtttctat gagatcagcg aacgcggcac ttcctggatc
     2161 ggaccgctgt tgtttggcct agtcacgcaa gcgaccaaca gtatgcgtac cgcgattttc
     2221 tcgttaatcg ttttgttcgt aggcggcctg cttgtcctga tcaccgtcaa tgttccgcgc
     2281 gcgattcatg aatcgggcaa tgacgttgaa ctgctccttg agcctgagac tgcgccggcc
     2341 taaggccatt tttgcactcg agtgtttgac atgcgatcgc actttggcat attgtataac
     2401 agccgctcat ccgggcggct gtttgcatgt gatatgattc gtggataacc ttccgaaggt
     2461 tagatttgtg cgaaccatag tcgatcacca ctaaaaccat cgcaaggatt taggagcatg
     2521 atgagtcaag catttgcagt cgagaccgcg catctacgca aagagtatgg gcgcaaggtt
     2581 gcagttgaag atttaaattt gcaagtgagg cccgctgaag tttttggctt tctgggtccg
     2641 aatggtgcgg gcaagacgac atcgatcaag atgctgctgg gcttggcgga gccgacgaaa
     2701 ggcgaagcgc ggctgctcgg taaaccgatc ggtgatccgg ccgcccgatc gcgcgtcggt
     2761 tttttgccgg aacattttcg tttccatgac tggctgacgg gcgaagaatt cttgcaagtg
     2821 catggtcaac tctatgggat gagcgagatc gatcggaagc agcgcgtgcc ggacttgttg
     2881 aagcgagtag gcttggaagc acatgcggat aagcgactgc gcggttattc gaaagggatg
     2941 ttgcagcgcg ttggcttggc tcaggctttg ctcaatcatc cggcggttgt ctttctcgat
     3001 gagccgacgt cgggtctcga tccgatcggg cgggtgttgg tgcgcgatct cattcacgaa
     3061 ctacgggacg aaggcacaac ggttttcctc aattcacatt tattatccga agttgaaatc
     3121 acttgtgatc gggtggtgtt tatcaagcat ggcagcgtgg tgaaagatgt ggtcctaaaa
     3181 gaattagagc tgggaccaat gcctgtgttg atcaaagtcg gagcattacc ttcaatcgaa
     3241 gatctgcgcc gcgacttgga acgttggggg catgatattt cgatcgaggc taatcatatc
     3301 cgcttgatcg tcgacgacga tagccactta cccgatctgg cgaggtggtt aatcgggcag
     3361 gggatcgatc tatattcgct gatgccgcag cacgtctcgc tcgaagaatt gtttttgcaa
     3421 atcgtcggcg cggaagagag tttgtgagat gcccattcca atcctcatcc gcttaacgat
     3481 ccgtgaagcg ctgcggcgca aagtcatatt gggactgttg atcctcggcg cggtgttcct
     3541 gattctgttc tcgttgggag tgggctttgc gcacggccaa attttggaat acggttcagg
     3601 cgcaactggc cgtctgccga tcagcagtgt ctacagcttc ttgctcacgt ccggcttgta
     3661 cgcggctaac ttcttgattg taatgttgag tgtgttggtc tcggtcgaca ccatatcggg
     3721 cgagatcagc accggcacga ttcaatcgat cgccgttaag ccgatccgcc gatcggatat
     3781 tttgttaggc aaatggctgg gtttgatgat catgctcggc gcgtgtgtct tatttctatc
     3841 gggcggcgtt attttgctca ccgcgttaat tactggctat gttccgccga atccgttggg
     3901 cgcgattgtc ttgatgttcc ttgaatcaat cgtgctatta tgcgcgtcgt tgttaggcgg
     3961 cacgcgctta tcgactttag cgaacggcgt gttcggcttt ggcctatttg gagccgcgtt
     4021 gatcggcggc ttcattgagc agatcggggc gtttctcaat aacgatgcgg ctctgacgat
     4081 cggtcacatc gcaacgatcg tcatgcccag cgatgcgtta tggcggttgg cgctgacgca
     4141 gactgctgaa ggggtgaatc cattccgttt gatgtttggc ggattatcag agcctgatcc
     4201 aaacatcgta gtttatgcgt tggtctttgc ggggatcgtt ttgttgctgg cgatacgatc
     4261 gttttcaaaa agagatttat agttccctca cgtctggcca actaatagtc attcccgcgc
     4321 aggcgggaat ccagtgcgtt agaatgacct acacaacgac aactttagtg ccttcgccgc
     4381 gcttgattgg tagaccatat tcggtgtaag gcactgctgg ttccgtccag cgaaagagcc
     4441 actccgcggc ttgatttgtg gcgttgacac aaccatgact gcgcggtcga ccaaaatcgt
     4501 tgtgccaata tgtgccatga gtggcgcaac cggaatatgt gaagtaaacc ggaaacgcca
     4561 cgccgggcaa gttgtagtag tccgagcctg cgccgccgat catataagc
//
LOCUS       DASRTJ010000590         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75292,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000590 DASRTJ010000000
VERSION     DASRTJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75292"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFF70_12030"
     CDS             <1..507
                     /locus_tag="VFF70_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZY45467.1"
                     /translation="LLIVLLAPAAILGDNVGYWFGAKAGPRLFNRENSRFFKKKNLLS
                     AKAFYEKHGGKTIVIARFMPFIRTFAPIVAGAGEMHYRRFVFFNFFGGLLWVLSMLLI
                     GFGLGKAAANVAPETVDKYFLILIVAVVFISALPTLIHLWKENRVEIMRRLRRQPAPS
                     PSVETSEE"
     gene            complement(550..1113)
                     /gene="def"
                     /locus_tag="VFF70_12035"
     CDS             complement(550..1113)
                     /gene="def"
                     /locus_tag="VFF70_12035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZY45468.1"
                     /translation="MTVREIKVIDDPVLRRKAKKIDKITPETRKLIEDMIDTMRAAPG
                     VGLAAPQVGSSERVIVVEYAEQDEKEETAPAAPPKMKKLYAVINPEIVWTTDEIIEGT
                     EGCLSIPGWTGDVLRHEAIVVKGLNRSGQRIKIKADNWLARIFQHEIDHLDGILYIDR
                     LIAPDRFRRLTQGDAETAAEKAAEAMG"
     gene            complement(1100..>2661)
                     /locus_tag="VFF70_12040"
     CDS             complement(1100..>2661)
                     /locus_tag="VFF70_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY45469.1"
                     /translation="LYIIQSGQVGLTHIDPQGAPNDVGMREAGAWLGESSLLLGEPHD
                     VTVRALNDVVAIAFSSKEFKELCDHTPGLFDRLTPREENASKINAPHYGWQGEDEAVV
                     IFVRQHKWALVRSMLVPIGALVVAFVVVAVLSQIIPTLTAFLVIVAVLIPLLIAFYVL
                     IDWSDDYYVVTNKRLVHVDELPIIRKRREEAPLSSITEIQFARHSILAHVLDFGDLRV
                     ETFGGGVSMRDIPHPNLVKEAIQREIERVKARARASERSAIRDELRQRIFARTVSATE
                     LENSESGSSPVEQPQPSIFQIIGGALGYFLPRLREVQGDTIIWRKHWIVLLQTSKWPL
                     LGLTITFMAFVIWWNHSVLLNWVPDNLWWLWVVIGLGLFAWYLWLFEDWRNDQYIITP
                     TRVIDIERRPFLMSETRREAALSKIQTTDLNIPSPSAQIFRYGNVSIRVPGAVIEFKF
                     VKDPAGVQAEITKRIAEFNRRVALNDARGRHTELSDWFAAYNQIQHQPQRPEIKRPPV
                     SPEEQSDHDSP"
BASE COUNT          585 a          756 c          671 g          649 t
ORIGIN      
        1 ctcctgatcg ttttgcttgc gcccgcagcg atcttgggcg ataacgtcgg ttattggttt
       61 ggcgcgaaag ctggtccccg cttattcaat cgcgagaact cacgtttctt taagaagaaa
      121 aatttgttgt cagcgaaagc cttttatgag aagcatggtg gtaagacgat cgtgatcgca
      181 cgcttcatgc cgttcatccg cactttcgct ccgatcgtag ctggtgctgg cgaaatgcac
      241 tatcgccgct ttgtgttctt caatttcttc ggcggattac tgtgggtgtt gtcgatgctg
      301 ttgatcggct tcggcctcgg caaagccgcc gcgaacgttg cgcctgagac ggtcgataaa
      361 tacttcttga tcttgatcgt tgccgttgtt ttcatttcgg cgctgccgac cttgatccat
      421 ttgtggaaag aaaaccgcgt agagatcatg cgccggttgc ggcggcagcc cgcgccctct
      481 ccttccgtcg aaaccagcga agagtaagca gcgaaaaacc aggcccctta atgagacctg
      541 gttttatttt tagcccatcg cttcggcggc tttttctgcc gccgtctctg catcaccttg
      601 agtaaggcgg cggaaccgat cgggagcgat cagccgatcg atatacagaa tgccgtccag
      661 atgatcaatc tcgtgttgaa agatgcgggc cagccaattg tccgccttga tcttgatccg
      721 ctggcccgat cgattgaggc ctttcaccac tatcgcttca tgccgcagca cgtcgcccgt
      781 ccagcccgga atacttaagc agccttccgt cccctcgatt atttcatcgg tcgtccatac
      841 aatctccgga ttgatcaccg catacagttt tttcattttc ggtggcgcgg ctggcgcggt
      901 ttcttctttc tcatcctgtt ctgcatactc gacgacgatc acccgttcgc tggaccccac
      961 ttgcggcgcg gccaggccta cgcccggcgc agcacgcatc gtgtcgatca tatcttcgat
     1021 caattttcga gtttccggcg tgatcttgtc gatcttcttg gctttgcgcc gcagcaccgg
     1081 atcgtcgatc actttgattt cacggactgt catgatccga ttgctcctct ggcgaaaccg
     1141 gcggccgctt tatctctggc cgctgcggtt gatgctgaat ctgattgtat gcggcgaacc
     1201 aatcgcttaa ctccgtgtgt cgcccgcgcg catcattcaa ggccacgcgc cgattaaatt
     1261 ccgcaatgcg tttggtaatc tcggcttgca cgccggccgg gtctttcaca aatttaaact
     1321 cgatcactgc gccgggcacg cgaatcgaca cattgccata gcgaaagatt tgcgccgatg
     1381 gcgaaggaat attcagatcg gtcgtttgaa ttttcgagag cgccgcttcg cgccgagtct
     1441 cgctcatcaa gaacggccga cgttcgatgt cgatgacgcg cgttggagtg atgatgtatt
     1501 gatcgttgcg ccagtcttca aaaagccata agtaccacgc aaagagaccc aggccaataa
     1561 cgacccacag ccaccagaga ttatctggaa cccaattaag caggacgctg tgattccacc
     1621 aaataacgaa agccatgaag gtgatggtca gaccaagcag cggccatttc gaagtttgca
     1681 gcaatacaat ccaatgtttg cgccagatga ttgtatcacc ctgtacttca cgcaaccgcg
     1741 gcaaaaaata gcctaacgcc ccgccaatga tctgaaatat tgatggctgt ggctgttcga
     1801 ctggactgga cccgctttca ctattttcca gttcagtcgc agacaccgtt cgagcgaaaa
     1861 tgcgctgccg cagttcgtcg cggatcgcag atcgttctga ggcgcgggcc cgtgctttca
     1921 cgcgctcgat ctcgcgctgg atcgcctcct tcaccaagtt agggtgagga atgtcgcgca
     1981 tcgacacgcc gccgccgaat gtttccaccc gcaaatcacc aaagtcaaga acatgcgcta
     2041 agatgctgtg ccgggcaaat tggatttcgg ttatggagga caaaggtgct tcttcacggc
     2101 gcttgcggat gataggcagc tcatcgacat ggaccaggcg tttattggtg actacgtaat
     2161 agtcatctga ccaatcgatc aaaacataga atgcaatcaa caacggtatt agcacagcca
     2221 caatcaccaa aaaggcggtt agagtaggga taatctgact tagcacggct acaacaacga
     2281 atgcaacgac aagggcaccg atcggcacca acatcgatcg aaccaacgcc catttgtgct
     2341 ggcgcacaaa aatcaccacg gcttcatctt cgccctgcca gccatagtgc ggcgcgttga
     2401 tcttgctcgc attttcttcc cttggcgtca gccgatcaaa gagacctggg gtgtgatcgc
     2461 agagctcttt gaattccttg cttgaaaacg cgatcgcgac cacgtcgttg agtgcgcgca
     2521 ccgtaacatc atgcggctct cccaacaaca gcgatgattc ccccagccag gcgcccgctt
     2581 cacgcatgcc gacgtcattc ggcgcgcctt gcggatcgat atgtgtgagc cctacttgac
     2641 ccgattgaat aatatataag g
//
LOCUS       DASRTJ010000591         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_75331,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000591 DASRTJ010000000
VERSION     DASRTJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75331"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFF70_12045"
     CDS             <1..384
                     /locus_tag="VFF70_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZY45470.1"
                     /translation="VCGQARFGYNRNMMKLMMFTRTFGCSDQKLARNCLAELNIRPIE
                     LNISIDRDAAQTLMKWVGSLSVPTLVVTDDHDQPIMPPQPLRPDQHTRNTDRGSMISE
                     PNCASLRLFLKKHGFISDHDHPSNT"
     gene            362..1153
                     /locus_tag="VFF70_12050"
     CDS             362..1153
                     /locus_tag="VFF70_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45471.1"
                     /translation="MITHQTLSIEGRSAKVWLGGSGRPLVLLHGGFGDAQQHWHATFQ
                     AFVPHFHIIAPDLPGFGVSAPLAAPNFQSYLSWINVLFEMLGLAGPLLMMGNSFGAAI
                     ARLYAAANTGEIDRLVLVDGGAIQDVGGCARSIFQLPIVSPFLLELARRQSFSNKGLR
                     KAIHVDSVFTPEFAANAHAASHGFVTSMKEIATNEPPSLRTPTCPTLIVWGEYDGLSS
                     TANGQRLSASIPHSEFALIKDAGHLPQIEQPQAFQAVVLKFLESK"
     gene            1282..>2187
                     /locus_tag="VFF70_12055"
     CDS             1282..>2187
                     /locus_tag="VFF70_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZY45472.1"
                     /translation="MFRKIFATGAVLLISGALSACNGSNGNLLPTVAPSAATATPAIS
                     IVPQITAQPTTDFSQFPTTVPGTPAPTPDIEKVINPQPDDWTRGAVSPTVTFIEWSDF
                     QCPYCSQFAPVLEKLLKAYPNDVRVVYRHFPLISIHDKALLAAEATEAAGAQGKFWEM
                     HDVIFSNHDIWENAAPADFRKTLDTYATQIGLDVKQFGTDLDGGKYEKKVMDAYNLAA
                     QLGLSGTPFILVNQNPWPNDLNYLSYNNVVGIVKLFAELPQKQFKQAPVMALDAKKSF
                     VATLKTDQGDIVIKLYADITPIAVNN"
BASE COUNT          516 a          601 c          558 g          512 t
ORIGIN      
        1 gtgtgtggtc aagctcgatt cgggtataat cggaacatga tgaagttaat gatgttcacc
       61 cgcacgttcg gctgcagcga tcaaaagctg gcgcggaact gtctggccga attaaacatt
      121 cggccgattg agctcaatat ctcgatcgat cgggacgcgg cacagacgtt gatgaagtgg
      181 gtgggatcgt tgagcgtgcc gaccttagtc gtgaccgacg atcacgatca accgattatg
      241 ccgccgcagc ccttgcgacc cgatcagcac acccgcaata ccgatcgcgg atcgatgatc
      301 agtgaaccaa actgtgcttc gctgcgactc ttcttaaaga aacacggatt catttctgat
      361 catgatcacc catcaaacac ttagcattga aggccgatcg gctaaagtct ggctgggcgg
      421 cagcggccga ccgctcgtat tactgcatgg cggtttcggc gacgctcaac aacattggca
      481 cgccactttt caagccttcg tgccgcactt ccatatcatc gcgcctgatc tgcccggctt
      541 tggcgtcagc gcaccgctgg ccgcaccgaa ttttcaatcg tatctgagtt ggatcaatgt
      601 gctgtttgaa atgttaggcc tggccggtcc gttgttgatg atgggcaatt cattcggcgc
      661 ggcgattgcc cgcttatatg ccgctgcgaa tacgggcgag atcgatcgat tggtgctggt
      721 cgatggcggt gccattcaag acgtgggtgg ctgcgcccga tcgatcttcc aactcccgat
      781 cgtcagtccg tttctgctgg agttggcgcg acgccaatcg ttttcgaata agggtctgcg
      841 taaagcaatc catgtcgatt cggtcttcac acctgagttt gccgcgaatg ctcacgccgc
      901 ctcgcacggt tttgtcacgt cgatgaaaga gatcgcgacg aacgaaccgc cctcattgcg
      961 cacacccacc tgcccgacgt tgatcgtctg gggcgaatat gatggactca gttcaacggc
     1021 taacggccag cgactttccg catcgattcc ccattcagaa ttcgcgttga tcaaagacgc
     1081 cggccacctg ccgcagatcg aacaaccgca agcctttcag gctgtggtgc tgaaattcct
     1141 cgaaagcaag tagcggattg cttatcgggt aaactgattc gtaatctcac ttctctcatt
     1201 catccctaag ttcttatcct ttccggtgtg gtacaatccg cctcgttgtg ttttagcaac
     1261 attcattcat gaaggagcaa tatgtttaga aagattttcg ccacaggtgc agtgctcttg
     1321 atcagcggtg cactgtcggc ttgcaacgga tcgaacggca atttactacc gactgtcgca
     1381 ccgtcggcag cgaccgcgac gccagcgata tcgatcgtgc cgcagatcac cgctcaacca
     1441 accaccgact tcagtcaatt tccaacaact gtaccgggca cacccgcgcc aacgccagac
     1501 attgagaaag tcattaatcc acaaccggat gattggacgc gcggcgcagt ctcgccgacg
     1561 gtaacgttca tcgagtggag cgatttccag tgtccgtatt gttcccaatt cgcacccgtg
     1621 ctggaaaagt tattgaaggc ttatccgaat gacgtgcgcg tcgtctatcg acattttccg
     1681 ttgatcagta ttcatgataa agccctgctg gccgctgaag cgaccgaagc tgcgggcgca
     1741 cagggtaaat tctgggaaat gcacgacgtc atattttcga accatgacat ctgggaaaac
     1801 gccgcgcccg ccgattttcg aaagacgctc gacacttacg cgacgcagat cggtctcgac
     1861 gtgaagcagt tcggcactga tctggatggc ggcaagtatg aaaagaaagt catggatgct
     1921 tacaatctag ctgcgcaatt aggcttaagt ggaacgccgt tcatcttggt gaatcaaaat
     1981 ccatggccaa acgatctgaa ttatttgtcg tataacaacg tggtcggcat cgtcaaactg
     2041 tttgccgaat tgccacagaa acaattcaag caagcgccgg tgatggcgct cgatgccaaa
     2101 aaatcgttcg tggcgacact caaaacagat caaggcgaca tcgtcatcaa actctatgcg
     2161 gacataaccc cgatcgccgt taacaac
//
LOCUS       DASRTJ010000592        10148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_356546,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000592 DASRTJ010000000
VERSION     DASRTJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356546"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="VFF70_12060"
     CDS             complement(<1..2027)
                     /locus_tag="VFF70_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZY45473.1"
                     /translation="MEAKDFSALRVSLASPEQIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTKDWQCYCGKYKNVRYRGIICDKCGVEVTRAAVRRERMGHIELAAPVAH
                     VWYTRRVPSYLGVLLDISRRNLDRVLYFAQYVVTHVDEEARLKALKRLDDEAERERKR
                     VESAGKEGTRAIKEKVEAELEKRKALRDERAGKLDEKLNSATEAVMKEAQKLQDRLDE
                     RMSKDIRASIEFEAAKITIAEAGETIGREHLIRLNKAVKAKLAELEAKNAEERDKSLA
                     KIESEIEYWKGASKDEIATIDTKIQEDLEAHRHKVDADREELKGLGTLQFLTEPRYRD
                     LKSRWGQVFKADMGAEAFLEILKRLELNQMSKELWREVKISKSKQKRKKAIKRLRVVE
                     SLRKSNNRPEWMILTLLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVIGPKLKLHQCGLPKTMALELYRPFVISKLVQYNFASNVKGAKR
                     IIERERPEVWEVLEEVIKQRPVLLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCSAF
                     NADFDGDQMAVYVPLLDEAQAEARDLMAANKNLLKPQNGDPIVHPRMDMVLG"
     gene            complement(2328..2522)
                     /locus_tag="VFF70_12065"
     CDS             complement(2328..2522)
                     /locus_tag="VFF70_12065"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HZY45474.1"
                     /translation="MTDEVRAYCMKEKKEQLMKDVVITTTANGRRAAQGICSSCGTKM
                     YKFLPKAAAEPAAEPMAKPE"
     gene            2746..3012
                     /locus_tag="VFF70_12070"
     CDS             2746..3012
                     /locus_tag="VFF70_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZY45475.1"
                     /translation="MPTYGYRCDKCGYVFEKIQKFSDESVKSCPHCKTRGKVKRTVHA
                     TGIVFKGAGFYKTDSRKSADTASEAKPAEPTPKVETPKAPVAAE"
     gene            complement(3087..3386)
                     /locus_tag="VFF70_12075"
     CDS             complement(3087..3386)
                     /locus_tag="VFF70_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45476.1"
                     /translation="MVKLLMSWDIQTGREAEYFQFIIQEFVPGLLKIGLQPTEVWFTA
                     FGERPQILAGGLASDLPAMQAIVNGSEWKELRTHLEEFVSGYAQKVVRAEGGFQL"
     gene            complement(3478..4359)
                     /locus_tag="VFF70_12080"
     CDS             complement(3478..4359)
                     /locus_tag="VFF70_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45477.1"
                     /translation="MQSRTLRSFITIRLFLLSLFVVLVAAACNQQPPSPLDAGRSALQ
                     SGDANKAITELEKLITDQPASAPAHLLLGQAYFKAGRKDDAKSQFQAGFSLDPAATLV
                     ITSTDAEEVFLTGNVHATLGQFDQALQAYSTTLQIDPSKAGAYTNIGVVYYQTGKLDI
                     AVQQFQQALAIDPKDADTNYLLGAAYVQKNDLENAAKFFQTALMLDPNSTAAYIGLGN
                     VDLLRKNYEQAVTNLQKALNLQPDSPEALFGLGKAYYLQGNNAAAITTLTQFLQLNPA
                     EPYKSDAQKILQQLGAK"
     gene            complement(4362..5024)
                     /locus_tag="VFF70_12085"
     CDS             complement(4362..5024)
                     /locus_tag="VFF70_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45478.1"
                     /translation="MPVDITWLGHSCFRLRDRAATVVTDPYGKDMGLMLPRVRTDIVT
                     VSHDADDHNNVKGVKGEYRVLDGPGEYEVSSVFVTGLDLHGDRKMAKKETALRNTVFL
                     FEFDDVTVCHLGDLNLVPTQAQVEEALGEVDVLLIPVGGGESLNASQAAEVVSLLEPH
                     IVIPMHYKVPGNDAKLDLVSKFLKEMGLDKITPLESLKVSRGGLPDETQVVLLELKSK
                     EE"
     gene            5121..5852
                     /locus_tag="VFF70_12090"
     CDS             5121..5852
                     /locus_tag="VFF70_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45479.1"
                     /translation="MQPFIAGSGQVNQIDSIARLSLPQADRTTYSDAQIDDYAGLSRR
                     SFPHRPPIHLSLRARFSSENILGTAGFGFWNHPFVPNSRWPTLPRAIWFFFASSPSNM
                     ALATDIAGFGWKAATIDATQPSALAIAPLAPIILLLNQSRKLYRRIWPFIERRLRIAE
                     QLIDPALMREWHTYSIEWEIDRAQLSIDDRIVLETDRSPRGPMGFIAWIDNQFAVVTP
                     RGRINFGLLKIDREQWLEIEQIEIK"
     gene            complement(5862..6608)
                     /locus_tag="VFF70_12095"
     CDS             complement(5862..6608)
                     /locus_tag="VFF70_12095"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZY45480.1"
                     /translation="MIEVIDCEQADELLPAYMLDVLDVEEAVAMAMHLRTCERHRIEW
                     QALRPIVESLALTAPVAEIPPTAIKQQLLSQVRSTPRIMHRPFITRQAAFAPILIAFV
                     VLLGVGGWALSLQSQVTTQQTQLDRIATWQKSIQQFFIGTNVQTLPVKFTDQFANDQA
                     TMYVANDRVALAVQGLPTLSGDQVYQCWWLSDANEVMPGSTFKTDANGMGVWAWQKPA
                     DSEYHILGITLENQSGLTQPQGPLVLRVEF"
     gene            complement(6646..7221)
                     /locus_tag="VFF70_12100"
     CDS             complement(6646..7221)
                     /locus_tag="VFF70_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY45481.1"
                     /translation="MDYSGLDDSALVALVARRDANALSELYDRYSRLVYSLALRVVGE
                     RNLAEEVVLDSFYSVWRAAGSFVEERGRFVGWLMSVARHRAIDELRRLKVRPEGTAVE
                     LDDSLKTPQPDGPEDVVEMLYQREIVREVLSHLPEPQRKALELAYFGGLTQQEIAVKT
                     STPLGTVKTRIRLGLLKMRDELRRAQDDQNI"
     gene            7300..8043
                     /locus_tag="VFF70_12105"
     CDS             7300..8043
                     /locus_tag="VFF70_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZY45482.1"
                     /translation="MSDKATIVSLVSNLMFATRIEDAIRSAGGSIVNPIDLGALQQAL
                     HEGVRMIIIDASAPEYDWPNWVRAIKNDPATDNIPIIVFGSHVDTAVRRTALDAGADR
                     YLARSNFFDGLTEFIANALRDRTDDPCSEPLPAGVIHGFVEFNAGQFFEQHETLELVW
                     RAELRPIRDLYRGILQIGVACLQIERGNPRGALKLIDRGTRWLQPFRPHCQGVEVDRL
                     LADTERLRIAISQSDLIERTLFPKVYYQE"
     gene            8224..8985
                     /gene="fabL"
                     /locus_tag="VFF70_12110"
     CDS             8224..8985
                     /gene="fabL"
                     /locus_tag="VFF70_12110"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HZY45483.1"
                     /translation="MDFTNKVAVISGSGRGIGRSIAINFARQGADIVVNFFRNRAPAE
                     ETAKEIEKFGRRALIVKADVGEEAGINKLIEETEKSFGGLDILVCNAASGYNRPVMEQ
                     KIKGWDWTLNINARSVLFLAQKAAPLMQKRGRGYIVSISSLGSIRVLPDYVVVGASKA
                     ALEAVTRYLAVELAPMGIVVNAVSGGIVETDALKHFRSMQTGNILVNAADRTPAGRIV
                     EPDDIANVVAYLCTPQAEMIRGQTIVVDGGHSLTI"
     gene            9165..9935
                     /locus_tag="VFF70_12115"
     CDS             9165..9935
                     /locus_tag="VFF70_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZY45484.1"
                     /translation="MSNTSIKHSPDGGASRTGQIIQHIERHQHGALSDIILGGQDGLV
                     NVLGVILGVAAATPDPRIVIAAGLAATFAESISMGAVAYTTTQTDRAHYLSELEREKI
                     EIRELPDREKQEIREIYQKWGLTGDKLDQVVDIICSNEDTWLSVMMAEELHLAPVEES
                     GALKSAAIVGFSAIVGSVIPLIPFLLVAPPLSLLTRPTAILVAIVVSALTLFAVGVYR
                     AKTTIGKPVRSGLQMAIIGTISALAGFLIGSLFGTPNV"
BASE COUNT         2403 a         2809 c         2536 g         2400 t
ORIGIN      
        1 ccgagaacca tatccatacg agggtgcacg atcggatcgc cgttttgcgg cttgagaagg
       61 tttttatttg cggccataag atcacgagct tccgcctgag cttcgtctag aagcggcacg
      121 tagacagcca tctggtcacc atcgaagtcc gcgttgaacg ccgagcagac caacggatgg
      181 atttgaatcg ctttaccttc caccagcacc ggctcaaagg cttgaatgcc caaacggtgc
      241 aaggtcggcg cacgattgag cagcaccggc cgctgcttaa tcacttcttc cagaacttcc
      301 caaacttccg gccgctcgcg ctcgatgatg cgcttggcac ccttcacatt gctggcgaag
      361 ttatattgaa caagtttgct gatgacgaat ggccgataca attctagcgc catggtcttt
      421 ggcaaaccgc attgatgcag cttcaacttc gggccaatca caatcaccga tcgaccggag
      481 tagtccacgc gtttgcccag caaattacgg cggaagcggc ctttcttacc cttaagcata
      541 tcgctcaagc tctttaactc acggcgaccg cggcggctaa gcgccttgcc gcgctgcgag
      601 ttatcgatca gcgaatccac ggcctcttga agcatgcgct tctcattgcg cacgattacg
      661 tccggcgcgc cgagttcgag cagccgcttc aagcggttgt tgcgattgat cacgcggcga
      721 tagagatcat tgagatcgga ggtagcgaag cggccaccat cgagctgcac catcgggcgc
      781 agatcgggcg gaatgaccgg cagcaaagtg aggatcatcc actccggtcg attattgctc
      841 ttgcgcaggc tttcgaccac gcgcagccgc ttaatggctt tcttgcgttt ctgcttgctc
      901 ttgctgatct tgacttcgcg ccacaactct ttcgacatct ggttgagttc aagccgcttc
      961 aagatttcca ggaatgcttc cgcgcccatg tcggccttga agacttggcc ccaccggctc
     1021 ttcaaatcgc gatagcgcgg ctcagttaaa aactgcaatg tccccagacc cttcagctct
     1081 tcgcgatcag catcgacctt gtgccggtga gcttcaaggt cttcttgaat cttcgtgtcg
     1141 atcgtagcaa tctcgtcctt tgaagcgccc ttccaatatt caatttcgga ctcgatcttg
     1201 gccagcgact tatcacgctc ctcagcgttc ttggcttcaa gttctgccag cttcgccttg
     1261 accgctttgt tgagacgaat taagtgttca cgcccaatcg tctcaccagc ttcagcgatc
     1321 gttatcttgg ctgcctcaaa ttcgatcgaa gcgcggatgt ctttgctcat tcgttcgtca
     1381 agtcgatctt gcagcttctg ggcttccttc attacagcct cagtcgccga attcagcttc
     1441 tcgtccagtt tacccgcgcg ttcatcacgc agggctttgc gcttctcaag ctctgcctcg
     1501 actttttcct ttattgcgcg cgtgccttcc ttaccggcag actccactcg cttgcgctcg
     1561 cgctcagctt catcatccaa ccgcttgaga gccttcaagc gcgcttcttc atcaacatgc
     1621 gttacaacat attgggcaaa gtacaacacc cgatcgagat tgcggcgcga aatatccaac
     1681 aagacgccta gatacgaggg cacacggcgc gtataccaaa catgcgcgac aggtgccgcg
     1741 agctcaatat ggcccatgcg ttcacgccgc acagccgccc gtgttacctc cactccgcac
     1801 ttgtcacaga tgatgccgcg atagcggacg tttttgtact tgccacaata gcactgccaa
     1861 tctttggtgg gaccaaagat ggcttcacaa aacaagccgt ctttctcagg ccgtaaccgc
     1921 cgatagttga tagtctccgg cttcaatact tcgccatatg accacgatcg aatctgttcc
     1981 ggtgaagcta aacttacacg caacgctgaa aaatcttttg cttccacgcg atgcctcctc
     2041 gctaaaaaga aaatctgccg aattcccgct cgttcaaccc acgaacacag ggcaagccaa
     2101 aagaagcgct ggaaggcttt ttctttttgc cttcagcgct cttgcctgtt gtgtactact
     2161 tcaattcaaa gcaggttgat tataacctgc tttacttctt tgtcaagtga cgatctaaga
     2221 taaagcgaat aaaacttcgg ggtgagctaa acccgatcgg tttacagtgg ccgatcaagt
     2281 tgtgccggcg gcctagcatt tgataagccg ccaacacagt aaatcgacta ctctggtttt
     2341 gccattggct cggccgcagg ctctgcagct gctttaggca ggaacttgta cattttggtg
     2401 ccacagcttg agcagatacc ctgggcagcc cgtcgcccgt ttgccgtagt tgtgatcacg
     2461 acatctttca ttaattgctc tttcttttcc ttcatgcaat atgcacgaac ctcatcagtc
     2521 atgataaatc cctccaagtc acgtatttcg attaggcaag taaccttgct ttccgatacg
     2581 ctcagtctac catgcttgaa cctagcctgc aaggaattcg acgcgagaac tttatgtcgt
     2641 aggaccgaac agttgcacaa ccgcttcgtc acaacgacct tgcgattcag gaatcagcaa
     2701 ggtataatcc atctgctaag gccaagacgg gagagacaag aaacaatgcc aacttatgga
     2761 tatcgttgtg acaaatgcgg atacgtattt gaaaagatac aaaaattcag cgacgagtcg
     2821 gtcaaatcct gcccacattg caaaaccaga ggaaaggtaa agcgcacggt gcatgccacg
     2881 gggatcgtgt tcaaaggtgc cggtttctac aaaacggatt cgcgcaaatc ggctgataca
     2941 gcgagtgaag cgaagccagc cgagccaacg cccaaagttg aaacgcctaa agcgcccgtt
     3001 gcggctgaat agtttaactg atcaaaaaga gcggactgga aaatattgct ccaatccgct
     3061 cttttttgat cagctcagtc ccatggctac aactgaaaac caccctcggc tcgaacgacc
     3121 ttctgtgcat agccgctgac aaattcctca aggtgagtgc gcagttcctt ccattccgag
     3181 ccattgacaa tcgcctgcat ggccggcaag tccgaagcga gtccccctgc tagaatctgc
     3241 ggccgctcac caaaggctgt gaaccagacc tcggtaggtt gcaagccaat tttcaataga
     3301 cccggcacaa attcttggat aataaactga aaatattctg cttcgcggcc tgtctgtata
     3361 tcccaactca ttagcagctt aaccattagc acctcagatt ccaatccagc tatcactact
     3421 ataagaagtg taccacatca acgcgtctgt ctgccttaag ccgaaagtgg ataccggcta
     3481 ttttgcgcca agttgctgca aaatcttttg cgcgtccgat ttgtatggtt cggcggggtt
     3541 gagctgcagg aattgagtca gcgtcgtaat tgcagcggca ttattacctt gcagatagta
     3601 ggctttgcct aaaccaaata atgcttcagg cgagtcgggc tgaaggttaa gtgccttttg
     3661 caaattcgtc acagcctgtt catagttttt gcgcagcaga tcgacgttac ccaggccgat
     3721 gtaagccgcc gtcgagttgg gatcgagcat caaggccgtt tggaagaact tcgcggcgtt
     3781 ctccaaatca tttttctgga cataggcagc ccctaataag taattcgtgt ctgcgtcctt
     3841 ggggtcgatg gcgagggcct gttgaaattg ctgaacggct atatccaatt taccggtttg
     3901 ataatacacc acgccgatat tcgtatacgc gcctgcttta cttggatcga tttgcagcgt
     3961 cgtggaataa gcctgcagcg cttgatcgaa ttggccgagc gtggcatgca cgttgcccgt
     4021 caaaaaaact tcttcggcat cggtagaggt tattactaac gtcgctgcag gatctaggct
     4081 gaaaccggct tggaattggg acttcgcatc gtccttgcgc ccagctttga aataggcttg
     4141 accgagtaga agatgcgccg gagcagaagc cggctgatcg gtaattaatt tttccagttc
     4201 agtaatcgcc ttgttcgcat cgccggattg aagcgcgctg cgaccggcat ccaatggcga
     4261 aggcggctgt tgattgcatg cagccgctac cagcacaacg aacaggctca agaggaacaa
     4321 tcggatagta ataaatgatc ggagcgttcg agactgcata gttattcctc tttgctcttc
     4381 aattcgagca agaccacttg agtttcatcg ggcaatccgc cgcgcgatac tttcagcgat
     4441 tccagtggtg tgatcttatc caaacccatt tccttcaaga acttgctgac cagatcgagt
     4501 ttggcatcgt tgccgggaac tttataatgc atcggtatga cgatgtgagg ttccagcaaa
     4561 ctgacgacct cggcggcctg ggacgcgtta aggctttccc cgccgccgac cgggatcaac
     4621 agcacatcca cttcgcccag cgcttcctca acctgtgcct gtgtcggcac caagttcaga
     4681 tcgcctaagt ggcagacggt tacatcatca aactcaaaca ggaacacagt attgcgcagc
     4741 gcggtctctt ttttcgccat ctttcgatcg ccgtgcagat ccagaccggt tacaaacacg
     4801 ctggaaactt cgtactcgcc cggaccatcc aacacccgat attcaccttt gacgcccttg
     4861 acgttgttgt gatcatcggc atcgtggctg accgtcacaa tatcggtgcg cacgcgcggc
     4921 agcatcaatc ccatgtcttt gccgtacgga tcggtcacga cggtcgccgc gcgatcgcgc
     4981 agacggaagc agctgtgacc taaccaagta atatcaactg gcatgcaaaa ctccatgaat
     5041 caagattgtc gtcaaaacta ttatacctaa atcgaagtgg aatgccaatc agtgaatgga
     5101 ctataataat aatcaaacac atgcagccct tcatcgccgg atcaggacaa gtcaatcaga
     5161 tcgattcgat cgcgcgcttg agtttgccgc aagccgatcg aacgacctac tccgacgcgc
     5221 agatcgacga ctacgctggt ctgtcacgcc gatcgtttcc gcaccgcccg ccgatccatc
     5281 tctcgctacg ggcgcgcttc tccagcgaaa atattttagg cacagccggc ttcggatttt
     5341 ggaatcatcc ttttgtgccg aatagccgct ggccgacgct accccgtgcg atatggttct
     5401 tcttcgcttc gtcaccttca aacatggcgc tggcgaccga tatagcgggc ttcggctgga
     5461 aggccgcgac gatcgacgca acacaaccat cagcgttagc aatcgcgccg ctcgcaccaa
     5521 ttattttgct gctgaatcaa tcgaggaaac tttatcgaag aatctggccg tttattgaac
     5581 ggcgcttaag gattgcggag cagttgatcg atccggcgct gatgcgtgag tggcatactt
     5641 attcgatcga gtgggagatc gatcgggcgc agctatccat cgacgatcgg atcgttctgg
     5701 aaactgatcg atcgccgcgc gggccaatgg gcttcatcgc ctggatcgac aatcaatttg
     5761 cggtcgtgac gccgcgagga cgaatcaact ttgggctgct gaagatcgat cgagaacagt
     5821 ggctggagat cgagcagatc gaaatcaaat aaaacgcgag gttaaaattc gactctcaaa
     5881 accagcggac cttgcggctg ggtgaggccg gactgatttt caagtgtgat gcctaagata
     5941 tgatattcgc tatcagcggg tttctgccat gcccacactc ccataccatt ggcgtctgtc
     6001 ttgaaggtgc tgcccggcat cacttcgttc gcatcggaca gccaccaaca ttgatacact
     6061 tgatcgccgc tgagtgttgg caaaccctgc acggcgagag cgacgcgatc attcgcgaca
     6121 tacatcgtcg cttgatcgtt ggcaaattga tcagtgaatt tgacgggcaa tgtctgcaca
     6181 ttcgtgccaa taaaaaattg ctggatcgat ttctgccagg tcgcaattcg atctaactga
     6241 gtttgctggg tagtcacctg cgattgcagc gaaagggccc agccgccgac gccaagcaac
     6301 accacgaatg caattaatat tggagcaaac gccgcctgac gggtaatgaa cggccgatgc
     6361 atgatacgcg gcgtcgatcg aacctgcgag agtagttgct gcttaatcgc agtgggggga
     6421 atttccgcaa cgggagcggt taaagccagt gattcgacaa ttggccgcag agcctgccat
     6481 tcaattcgat gacgttcaca ggtgcgcaaa tgcatggcca tggccacggc ctcttcaaca
     6541 tccaacacgt ccaacatata ggccggcaat aactcgtcag cttgttcgca gtcaatgacc
     6601 tctatcacaa ttcactccac ccactattac gcacaaactt tagtcttaga tgttttggtc
     6661 atcttgcgcg cgccgtagtt cgtcacgcat cttcagcaat cccagtcgaa tgcgcgtctt
     6721 gacggtgccc agcggtgtcg aagtcttcac cgcgatctct tgctgggtca ggccgccgaa
     6781 ataggccagc tctaaagctt tgcgctgcgg ctcgggcaaa tggctcaata cctcacgcac
     6841 aatctcgcgc tgatacagca tttccacgac atcttccggg ccatccggct gcggcgtctt
     6901 gagcgaatca tctaactcca ccgccgtgcc ttccggtcga actttcaagc ggcgcagctc
     6961 gtcaatcgcg cgatgccgcg ccacgctcat cagccagccg acaaatcggc cgcgctcttc
     7021 gacaaacgat ccagctgcgc gccagacgct gtaaaacgaa tccagtacga cctcttcggc
     7081 cagatttcgt tcgccaacga cacgcagcgc cagactatac accaagcggc tgtagcgatc
     7141 gtacaactcg ctcagcgcgt tggcatcacg acgggccacg agtgccacta aagcagaatc
     7201 atccaagccg gagtaatcca cgactcgatt ctatcacaaa caagcattaa cttgtaaact
     7261 gcattgtgtg aggtgtgcga ttgcggtaca attcaactcg tgagtgataa ggcaacgatt
     7321 gttagtctag tctcgaattt gatgtttgcc acgcggatcg aagacgcaat tcgatccgcc
     7381 ggtggctcga tcgtaaaccc gatcgatctc ggcgcgcttc aacaggcact gcacgaaggc
     7441 gtgcgcatga ttatcatcga tgccagtgcg ccagaatatg attggcctaa ttgggtgcgc
     7501 gcgatcaaaa acgatcccgc gacggataat attccgatca tcgtctttgg atcgcatgtc
     7561 gatactgcgg tcaggcgaac ggcgttagat gccggagccg atcggtatct ggcccgatcg
     7621 aatttttttg acggcctaac agagttcatt gccaatgcgc tgcgcgatcg aacggatgat
     7681 ccgtgcagcg aaccgctgcc cgctggcgtg attcatggtt tcgtagaatt caatgcgggc
     7741 cagttcttcg agcagcatga aacgttggag ttggtgtggc gcgccgagct gcgccccata
     7801 cgcgatctct atcgaggcat attgcaaatc ggtgtagctt gtttgcagat cgagcgtggc
     7861 aatccgcgcg gcgctttgaa gttgatcgat cgcggtacgc gttggctgca accctttcgc
     7921 ccccactgtc agggcgtgga agtcgatcga ttgttggcgg atactgagcg gctgagaatc
     7981 gccataagcc aatcagattt aattgaacgc actctctttc cgaaagttta ttatcaagaa
     8041 tgatctgtca tttcgggcga agcgagaaat ttcagatact atcagcatca attgctaatc
     8101 accatagaga tttcggccat tcctaatgcc attgggcatt ggtggcccgc taggcgtcgg
     8161 cctgcggcct cgaaatgaca actgacccgc gatctgtaac ttcaatctaa ccggaggata
     8221 cacttggact tcacgaacaa agtcgccgtc atcagcggat cgggtcgcgg catcggccga
     8281 tcgattgcga ttaacttcgc caggcagggc gcagatattg tcgtcaactt ctttcgcaat
     8341 cgcgcacctg ctgaagagac cgctaaagag atagagaaat ttggccgacg cgcgctgatc
     8401 gtaaaggccg atgtcggcga agaagcgggc atcaacaaac taatcgagga gaccgaaaaa
     8461 tctttcggcg gcctcgacat cctcgtgtgc aatgcagcct ccggttacaa tcgaccggtg
     8521 atggagcaga agatcaaagg ctgggattgg acgttgaaca tcaacgctcg atcggtgttg
     8581 ttcctcgcgc agaaagccgc gccgctgatg cagaagcgcg gacgcggtta catcgtttcg
     8641 atcagcagcc tcggatcgat ccgcgtgctg ccggattacg tcgtcgtcgg cgcaagcaaa
     8701 gccgcgctcg aagccgtcac gcgatacttg gctgtcgaac ttgcgccaat gggcatcgtc
     8761 gtcaatgcgg tgtcgggcgg catcgtcgag acggatgcgc tgaaacattt tcgatcgatg
     8821 cagactggaa atattttagt gaacgccgcc gatcgaacac ccgccggtcg catcgtcgag
     8881 ccggacgata ttgcgaatgt cgtcgcgtat ttgtgcacgc cgcaagccga aatgatccgc
     8941 ggtcaaacga tcgtcgtcga tggcggacac agcttaacga tttaatcgtc attgcaatct
     9001 ccattcgcaa tggcggttaa gatgaggtgg cggggcaatc atctcgccgc tttttgttga
     9061 ccgggacttc ataaccacta agtcacaaag acacgaagaa aacaaaaaaa tactttgagc
     9121 cttagtgcct tagtggttaa tcaaccactg taaatggatt cgcgatgtca aacacttcaa
     9181 tcaaacatag tcccgatggc ggcgcatcgc gcaccggcca aatcattcag cacatcgaac
     9241 ggcatcaaca tggcgcattg agcgacatca ttctcggtgg ccaagatggt ttggttaacg
     9301 tgctcggcgt catacttggc gtcgccgccg ccacgccgga tccacgcatc gtcatcgccg
     9361 ccggacttgc cgcgacattc gctgaatcga tctcgatggg cgcggtagcc tacacaacca
     9421 cccaaaccga tcgcgcacat tacttgtccg aactcgaacg cgagaagatc gaaatccgtg
     9481 agctgcccga tcgggagaag caggagattc gggagatata tcagaaatgg ggactaaccg
     9541 gcgacaaact cgatcaagtc gtggacatca tctgctcgaa cgaagatacc tggctgtcgg
     9601 tgatgatggc tgaagaattg catcttgcac cggtcgaaga aagcggagca ttgaaaagcg
     9661 cagcgattgt cggtttctcc gcgatcgtcg gttcagtgat cccgttgatt ccattcttgc
     9721 tcgtcgcacc accgctaagt ctactcaccc gaccgaccgc tatcctcgtc gcgatcgtcg
     9781 tctcggcctt gacgttattt gccgttggtg tctatcgagc gaagacgacg atcggcaagc
     9841 cggtgcgcag tggactacaa atggccatca ttggcaccat ctcggcgtta gccggtttct
     9901 tgattggatc gctatttggc acaccgaatg tataatttca aacacgattg aaattattga
     9961 cacgccgcta aatcgagagt aatatcttca acgagacttt acctcaacca catcagccgg
    10021 tcatccactg aataacgggc tttaaccttc atcatagcca atgggggatt tgcatgaaac
    10081 catcacgcgc tgcatcgacg atcgttttag gtttattcgg attaattgtg ttgttccttg
    10141 cgctgccc
//
LOCUS       DASRTJ010000593         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_340516,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000593 DASRTJ010000000
VERSION     DASRTJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340516"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFF70_12120"
     CDS             complement(<1..271)
                     /locus_tag="VFF70_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45485.1"
                     /translation="MDLTHFPLVTLVVFLPTIGALIVAFENKEDSNTLKQTALIVSLL
                     TFVVSLGFVFGGFKAELSGFQFTEKYAWIPAFNINYEVGIDGISLL"
     gene            complement(310..>1728)
                     /locus_tag="VFF70_12125"
     CDS             complement(310..>1728)
                     /locus_tag="VFF70_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZY45486.1"
                     /translation="MGEVAPGLAVLVTLLLLVGVTGKSAQIPLYVWLPDAMAGPTPVS
                     ALIHAATMVTAGVYLIARSFPLFHLVPDVQAFVGILGAVTALFAGSIAVAQFDIKKVL
                     AYSTVSQLGFMVSAVGMGATVAGIFHLMTHAFFKALLFLGAGSVIHGVEHGHHLAHEA
                     NAHGDAHSSDDHHHDEKPFDPQDMRNMGGLWHKMRTTSIVYIIGALALAGIFPLAGFW
                     SKDEILAEALDKSPIAFVLLLIAAAFTAFYMTRQVLMVFFGKPRSEAADHAPESSNWM
                     TRPLIVLAFFAVTIGFINIAGGFTHWLEPSEEFPGLNLLVAGGSTLIAVLEIFFGYLI
                     YRPRTEAAKLDDPLRKTGFIFMILNNKYWIDQIYDRLFVRPFKWLSGFLANVIDWRFW
                     HDFFHNTIIWGGFMKLTSFLANPIDQLVIDGAVNGTGRLIAGTSARLRKIQTGFVRNY
                     ALMMLVGVAIVLVWFATRVFVK"
BASE COUNT          469 a          486 c          444 g          329 t
ORIGIN      
        1 gcagcagact gataccatcg atgccgactt cgtagttgat attgaacgcc ggaatccacg
       61 cgtacttttc ggtgaattgg aaacccgaga gttcagcctt gaatccgccg aacacgaagc
      121 caagcgagac gacgaacgtc agcagcgaca cgatcagcgc tgtttgcttc agcgtgttgc
      181 tgtcttcctt gttttcgaat gcgacgatca gcgcgccgat cgtcggcaga aagaccacca
      241 gtgtgacaag cgggaagtga gttaaatcca tagttgcctc aagaatcagt gaatagtaat
      301 gcgtaagtgc tactttacaa agacgcgagt ggcgaaccac acaagaacta tcgccacacc
      361 gaccaacatc atcaaagcat agttgcggac aaagcccgtt tgaatcttgc gcagtcgtgc
      421 tgatgtgccg gcgatcaaac ggcccgtgcc gttgaccgca ccatcaatga ccagctgatc
      481 gatcgggttg gccaggaacg acgtgagctt catgaagccg ccccagatga tcgtgttgtg
      541 gaagaaatca tgccagaagc gccaatcgat cacgttggcc aagaagcctg atagccactt
      601 gaagggtcgc acgaacaatc gatcgtatat ctgatcgatc cagtatttgt tattgagaat
      661 cataaagatg aaaccagtct tgcgtagcgg atcgtcaagc ttggcggctt cagtgcgcgg
      721 ccgatagatt aagtaaccga agaagatctc taacactgcg atcaaagtcg atccgcctgc
      781 gactaaaaga ttcaaacctg gaaattcttc agaaggttca agccaatgcg tgaaaccacc
      841 cgcaatgtta atgaaaccga tcgtgacggc gaagaacgcg agaacgatca atggccgcgt
      901 catccagttc gaactttcgg gtgcgtgatc ggccgcttcg ctgcgcggct taccgaagaa
      961 gaccattaat acttggcgcg tcatatagaa cgcagtgaaa gccgcggcaa tcagtaacaa
     1021 cacaaatgcg atcgggctct tgtccaatgc ttcggccaag atttcatctt tgctccagaa
     1081 accggccagc ggaaaaatcc cggcgagcgc caacgcgccg atgatatata cgatcgatgt
     1141 cgtacgcatc ttgtgccaca agccgcccat gttgcgcata tcctgcggat cgaacggctt
     1201 ctcgtcgtga tgatgatcat ccgaagaatg ggcatcccca tgcgcattcg cttcatgcgc
     1261 aagatgatgc ccatgctcga cgccgtgaat caccgagccc gcacccagga acaacaaggc
     1321 tttgaaaaag gcgtgcgtca tcaaatggaa gatacccgca accgtcgcac ccataccaac
     1381 ggcactgacc atgaaaccga gttgcgaaac cgtcgagtaa gccaatactt tcttaatgtc
     1441 gaactgtgcc acggcgatcg atccggcgaa cagtgcagtc accgcgccga gaattccgac
     1501 gaacgcttgc acatccggaa ccagatggaa cagcgggaac gatcgggcaa tgagataaac
     1561 gccagccgtc accatcgtcg cggcatgaat cagcgccgag accggcgtcg ggccggccat
     1621 cgcgtcgggc agccacacgt acaacggaat ctgcgcgctc ttaccggtca cgcccaccaa
     1681 caacaagagc gtcaccagca cggccagacc cggagcgact tcacccat
//
LOCUS       DASRTJ010000594         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_281179,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000594 DASRTJ010000000
VERSION     DASRTJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281179"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFF70_12130"
     CDS             <1..221
                     /locus_tag="VFF70_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZY45487.1"
                     /translation="IDHIISLELGGSNSIRNLFPEPYTGDWNARIKDKLENKLHSLVC
                     SGQVDLVTVQQEISTDWINAYIKYIGQP"
     gene            243..539
                     /locus_tag="VFF70_12135"
     CDS             243..539
                     /locus_tag="VFF70_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45488.1"
                     /translation="MAIHANRKLTKLIAGRTIASTELQDKLLIHFTNGSIIKIKTGAP
                     WQGDVAQRIIKGVRQTDEVISFDFTDKTSMDVRLAEPGSSVMLRDGAGVMEYAD"
     gene            546..860
                     /locus_tag="VFF70_12140"
     CDS             546..860
                     /locus_tag="VFF70_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HZY45489.1"
                     /translation="MIPTINRGTAGKSISNETVYRAKWIIVNPGKRLSLQKHQHGSEQ
                     WVVVQGSGQFTLNDQVRSIKTGDSLFIPPGAIQRVQNIGTNDLIIIETQLGLCLEDDI
                     IR"
     gene            913..1518
                     /locus_tag="VFF70_12145"
     CDS             913..1518
                     /locus_tag="VFF70_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HZY45490.1"
                     /translation="MTRLLLIRHGHNDWADKKLAGWLPDVHLNELGQRQAQELVERLT
                     PLKIAAIYASPLERTIETAQPLAKARGLRIFKMRGLGEVDYGDWAGKSLKVLSQKKEW
                     RIVQVAPSAFRFPNGESMREVQQRAVATVEKIALAHPKETVAVFSHGDVIKSIVAFFS
                     GIALDNFQRIGISPAAISVIDLGPFGARINRLNDTGPFRSQ"
     gene            1526..2083
                     /locus_tag="VFF70_12150"
     CDS             1526..2083
                     /locus_tag="VFF70_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HZY45491.1"
                     /translation="MTDNIELTSVSAITADAVGQPGQRVFYVQARQGLTTVTIETEKE
                     QVRALSLGIHQFLEELIQRFPDRTELDRVDPRDLDLTPPFEPEFKVAQMGLGYDAETD
                     RVVIVAEALNTETEDEPAEEASEESGPKARFWITRGQAKALADHALEIVAQGRPLCPL
                     CGRPMNAEGHFCPKRNGHTKDLIVP"
     gene            2120..2917
                     /locus_tag="VFF70_12155"
     CDS             2120..2917
                     /locus_tag="VFF70_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HZY45492.1"
                     /translation="MSLSKFQPPNPTSTMPSPTNDSTPLSIDRVLDFLTHGKIEAKQL
                     LPWGSNATLLVTIKHSDLESLAIYKPRDGENPLWDFPEGTLCNREVAAFVVSQALDWL
                     IVPPTILRAGPQGIGMLQFYIDCDPNEHYFTLRDDDRFTDQFQKIVLFDLLINNADRK
                     SGHCLRAASDHVWAIDHGVCFSIEPKLRTVMWDYAGQPIPIGLRDDLKSLHARLDQPG
                     DPAIQALNELLDRREITALKRRATQLIASDCFPRPQPGYRQIPWPPV"
     gene            3049..>3752
                     /locus_tag="VFF70_12160"
     CDS             3049..>3752
                     /locus_tag="VFF70_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZY45493.1"
                     /translation="MATRTNPQQSALISNPPADERATVLHTWMIQSQWNTPTIVSGQG
                     AWFIDADENRYLDLSSMAECCNLGHQHPKVIAAIKAQADQMCYVQPGWGSQPRAELAQ
                     RLIDKSQMQNGKVFFTLAGADANENAVKFARWFTGRSKIITRYRSYHGATHAALDLGG
                     DNRGWHTQTGFDGIIHALPPYCYRCPFGLKYDSCNIHCADHIADLIEYAGPENVAAVM
                     IEPHAGTNGIVAPIEY"
BASE COUNT          889 a         1088 c          990 g          785 t
ORIGIN      
        1 aaatcgatca catcatcagc ctggaactcg gaggctcgaa ttcgatacga aatctgtttc
       61 cagagccgta taccggcgac tggaatgcgc gcatcaaaga caaactggaa aataaattgc
      121 acagtctggt atgcagcggc caagtagatc tggtgaccgt ccagcaagaa atctctaccg
      181 actggatcaa cgcgtacatc aagtatatcg gtcagccgta accgatcgat cggagtgaga
      241 gcatggcaat ccacgcaaac aggaaactga ctaagctcat cgctggacgc accattgcct
      301 cgactgagct gcaagacaag ctgctgattc actttaccaa cggatcgatc atcaagatca
      361 aaaccggtgc gccgtggcag ggcgacgtcg cgcagcgcat catcaaaggt gtgcgccaga
      421 cagacgaagt gatcagcttc gacttcaccg acaaaacctc gatggatgtg cgcctcgctg
      481 aacccggatc gagcgtgatg ctgcgcgatg gcgcaggcgt catggaatat gcagattaag
      541 tgtgcttgat tccgacaatc aatcgtggga ccgctgggaa gagtatctca aatgaaaccg
      601 tttatcgcgc caagtggatc atcgtcaacc ccggcaaacg gctatctctt cagaagcatc
      661 aacatggcag cgaacagtgg gtcgtcgtgc aaggctctgg ccagttcacc ctcaacgatc
      721 aagttcgatc gatcaagacc ggtgactcgc tgttcatccc accgggcgcg atccagcgtg
      781 tgcagaacat cggcaccaat gatctcatta tcatcgaaac ccaattgggc ttgtgcttgg
      841 aagatgacat catccgctaa gaggatgatt ggaatcggaa atagcgtatg gtacaatctt
      901 ctccacacag ttatgacccg actactcctc atccgtcacg gccacaacga ttgggccgac
      961 aaaaaactcg ccggttggct gccggacgtg catctcaacg aactcggcca gcgccaggcg
     1021 caggaacttg tcgaacgctt gacgccgcta aaaatcgccg cgatttatgc cagcccactg
     1081 gaacgcacga tcgagaccgc gcagccgtta gccaaagcgc gcggcctgcg gattttcaag
     1141 atgcgcgggc tgggcgaagt cgattacggc gactgggctg gcaagtcatt gaaagtcttg
     1201 tcgcaaaaaa aggaatggcg catcgtgcaa gtggcaccca gcgcctttcg ttttcccaac
     1261 ggcgaatcga tgcgcgaggt gcagcagcgc gccgtcgcca ccgtcgaaaa gatcgcgttg
     1321 gcgcatccta aagagaccgt ggcggtgttc tcacatggcg atgtgatcaa atcgatcgtc
     1381 gctttttttt ccggtatcgc gctcgacaac tttcaacgga tcggcatcag cccagctgca
     1441 atcagcgtga tcgatctggg gccgttcggc gcgaggatca atcggttgaa cgacaccggc
     1501 ccatttcgat cgcagtaggc acagcatgac tgataacatt gaactcacct ccgtctcagc
     1561 gatcactgcc gacgccgtcg gccaaccggg ccagcgcgtg ttctacgtgc aggcgcgtca
     1621 aggtttaacc accgtcacga tcgagaccga gaaagaacaa gtccgcgcgt tgtcgctggg
     1681 catccatcaa tttttggaag agttgatcca gcgctttccc gatcgcactg aactcgatcg
     1741 agtggatccg cgcgatctgg acttgacgcc gccgttcgag ccggaattca aagtggcgca
     1801 gatgggtctg ggctacgacg ccgaaactga tcgcgtcgtc atcgttgcgg aagcattgaa
     1861 cactgaaact gaagacgagc cagcggaaga agcgagcgaa gaatccggcc caaaggctcg
     1921 cttctggatc acgcgcggtc aagccaaagc cctggccgat catgctttag aaatcgtagc
     1981 gcaaggccga cctctctgtc cgttgtgtgg ccgccccatg aatgccgaag ggcacttctg
     2041 ccccaagcgc aacggacaca ccaaagattt gattgtgccc tagcggttag aggttggaaa
     2101 tttgaagttg gatgaatccg tgtcgctatc caaattccaa cctccaaatc caacttccac
     2161 catgccttca cctaccaacg attcaactcc cctctccatt gatcgcgtgc tggattttct
     2221 cacgcacggc aaaatcgaag cgaagcagtt gttgccatgg ggcagtaacg ctacactgct
     2281 cgtcaccatc aaacactcgg acttggaatc gctcgccatt tacaaaccgc gcgacggtga
     2341 aaatccgttg tgggattttc ccgaaggcac attgtgtaat cgcgaagtcg ccgcgtttgt
     2401 ggtcagccag gcgctcgatt ggttgatcgt gcccccgacg atcttgcgcg ccgggccgca
     2461 gggtatcggc atgctgcaat tctatatcga ctgcgatccc aacgagcatt acttcacgct
     2521 gcgcgatgat gatcggttca ccgatcaatt tcagaaaatt gttttattcg atctcttaat
     2581 caacaacgcc gatcgcaaat ccggtcactg cctgcgtgct gccagcgatc acgtctgggc
     2641 gatcgatcat ggtgtatgct tttcgatcga gcctaagctg cgcaccgtga tgtgggatta
     2701 tgcgggccag cccatcccga tcgggttacg ggacgatctc aaatctctcc acgcccgatt
     2761 ggatcagcct ggcgatccgg cgatacaagc gttgaatgaa ttactcgatc gacgcgagat
     2821 caccgcgctg aaacgccgcg ccactcaact gatcgcgtcc gactgttttc ctcgtccgca
     2881 gcccggctat cgacaaatcc cctggccgcc cgtttgaaat attgctccga gcgtagcgcc
     2941 agctccgccg cgagagctga cgctctcgct tgagcattca aaaatctgcg ggaggttatt
     3001 ccctgcttcg cttgccgcct ctcaacgcac ctgatacaat agcccgtgat ggctactcga
     3061 acaaatcctc agcagtccgc gctcatttcc aacccgcccg ccgatgaacg cgcgaccgtt
     3121 ttgcatactt ggatgattca atcgcaatgg aacacgccga cgatcgtcag cggtcaaggt
     3181 gcttggttca ttgatgccga tgagaatcgt tacctcgatc taagcagcat ggctgaatgc
     3241 tgcaacctcg gccatcaaca tccgaaagtg atcgcggcta taaaagctca agccgatcaa
     3301 atgtgttacg tgcagccagg ctggggctcg cagccgcgcg ctgaattggc ccaacgcttg
     3361 atcgataagt cgcagatgca gaatggcaag gtgtttttta ctttagccgg agccgatgcg
     3421 aacgagaacg cggtcaagtt tgcccgatgg ttcaccggac gctcgaagat catcacgcgt
     3481 tatcgatcgt atcacggcgc gacccacgcc gcgctcgatc tcggcggcga caatcgcggc
     3541 tggcatactc agaccggctt cgatggaatc attcacgcgc tgccacctta ttgctatcga
     3601 tgcccattcg gcttgaagta tgattcctgc aacatccatt gcgccgatca catcgccgac
     3661 ctgatcgaat atgccgggcc ggagaatgtc gccgcggtga tgatcgaacc gcacgcgggc
     3721 accaacggca tcgtcgcgcc gatcgaatat tg
//
LOCUS       DASRTJ010000595         6219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_37600,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000595 DASRTJ010000000
VERSION     DASRTJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37600"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFF70_12165"
     CDS             <1..292
                     /locus_tag="VFF70_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45494.1"
                     /translation="QFDLHLAWAPSTGMVRATYRAEIERYESKGDRFQCKLIELVAAS
                     GLQSDSELTRAMISNLIGQHVLVPREGVEGMILRLKMTTLTGGLKRPYFFNA"
     gene            356..1846
                     /locus_tag="VFF70_12170"
     CDS             356..1846
                     /locus_tag="VFF70_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZY45495.1"
                     /translation="MVVKKATKKAAPKKIAAGKNGSSNGKFKLTYGTMFNPPESLHTN
                     FDKAVAKFRGELGKDIPMFINGQDRVKILRKAAAQIEKRLYEISAIVSIEIGKSRMEA
                     LGDVQETADLISYYCDQMDANGGYIKQMGKDPLVGYNATNFSVLKPYGVWVVISPFNF
                     PFALAGGPTGAALVAGNAVVFKPASDTPYSGRLLAECYRDAGVPEGVFNYVTGGGSTI
                     GQELIANPMVDGITFTGSYDVGMHIYHTFATGKYPRPCIAEMGGKNPAIVSRNADLDL
                     AVQGIVRSAFGLQGQKCSACSRVYVDNAVKDKFVQKLVEATNKLKIGDPTLRENFLGP
                     VANQSSYQQYGEFSEELSHDGQILTGGKKLIEGDYGKGYFCAPTLAELPTNHRLWKTE
                     MFLPITTVAGYDDIHHAMELANDADYGLTAGFYGSQKELQWFFDNIEAGVTYANRPQG
                     ATTGAWPGFQPFGGWKGSGSTGKAGGSLYYVPQYMHEQSQTIIEKA"
     gene            2086..3303
                     /locus_tag="VFF70_12175"
     CDS             2086..3303
                     /locus_tag="VFF70_12175"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZY45496.1"
                     /translation="MSIQYIVLGAGRQGTASAYDLAKFGGADRVTMADVDRFVAQAAA
                     DRVNQLIGRSIADAIALDVKDDQAVRQAIKGYNVVLSAVPYFFNLNLTKIAIECGVSF
                     CDLGGNTEIVRQQHGLDAEAKRAGVTVVPDCGMGPGMGNTLAVYAMELLDKTDHIYLF
                     DGGLPIEPQAPWNYVESFSLEGLTNEYHDGMMVLRNSELTTIPPFSEMEIVNIPPLGK
                     MEAFIVAGGVSTTPWTFQGKLKTLQLKVLRYPGTFAQLKAYSDLGLYDFKPIKVDGVD
                     VIPRRVFHTLYGPHVHADVVKDICIVRAHAIGRKNGQRAEALIDLVDRYDPHTGFSSM
                     ERTTGWHLSIMAAMIAHKQIAAGAVPLELAVPGHAFVAEAKIRGFKIKTKVTTAKAKA
                     IAKSRTKSKLRKN"
     gene            3385..4350
                     /locus_tag="VFF70_12180"
     CDS             3385..4350
                     /locus_tag="VFF70_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45497.1"
                     /translation="MQLRKFFLFSLIIAAALAACNNPGPIDVSNPIGFKQKSGLFALQ
                     VPKSWTSSQDEVSTEAIASFADTTHRAELIGYVGLLDHQLSDAEGLQIAADLAGNLLN
                     QPGDLKITDKYRQPDGAFVAVFTFTRAGAPRTGQAIFRDADLSLAGTLINGPVDGWAD
                     LQKSLQPFVDSFKVDKDFIQGTYFTPIDTDAYAVVVPVGWAQQKSKTNNEVEVKAPNG
                     RLSIIGVQLIVTNTLDDTALANQAVSILKQAYRLDNTLSDTSKLPDGRLRLGLDRTDR
                     HTVGYVEQKDGYFIGLFFDVPTQQLLAYQSFIDFVYSTFVTGKAK"
     gene            4424..5326
                     /locus_tag="VFF70_12185"
     CDS             4424..5326
                     /locus_tag="VFF70_12185"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZY45498.1"
                     /translation="MSKVMSLPDAIAKFVHDGDTIYAAGFTHLIPFAAGHEIIRQRKN
                     DLVLARATPDLIYDQMVAAGCAKKIIFSYSGNPGVGSLRVIRQEMEAGRLAYEEYSHF
                     AMITRVAAGASGLPFLPMRPTGAEDLAKVNPQYKTIIDPYTGVELVTVPALNPDVAII
                     HAQRADANGNAQIWGIVGEQKEVAFASKKVILTVEEIVDESVIRSDPNRTLIPGFVVS
                     AVCHVPFAAHPSYTQGYYDRDNKFYLEWDKISETRENVTAWLDEWVYGLKDHAEYWAK
                     LGSEAHDRLKVQAAFSEPVNYGRY"
     gene            5460..>6219
                     /locus_tag="VFF70_12190"
     CDS             5460..>6219
                     /locus_tag="VFF70_12190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZY45499.1"
                     /translation="MNYSSSEMMIVAASRLLRDNDVVFVGIGQPNLACNLARRTHAPN
                     LNMIYEAGVIGAQPARLPLSIGDPCLVSGAASICSMVEIFAYYLQNGKIDVGFLGGAQ
                     IDRFGNVNATVIGSYDHPKVRLPGSGGSAEIAAWASRVYYITPHQARRFPEKCDFITS
                     AGFLAGRSDRAAKGLRGSGPQAVVTDLGLLTPDPDSGELVLTALHAGATVEQAIKNTG
                     WPLKVASALKTTEPPTAEELRLLREELDPHGMYLK"
BASE COUNT         1474 a         1663 c         1766 g         1316 t
ORIGIN      
        1 ccaattcgat ctccacttag cctgggcacc atcgaccgga atggtgcggg caacttatcg
       61 ggcggagatc gaacgatacg aatcgaaagg cgatcggttt cagtgcaagt tgatcgaact
      121 ggtggcggcg agcgggcttc aatcggattc tgaacttacg cgggcgatga tcagtaatct
      181 gatcggacag catgtgttgg ttccgcgcga aggcgtggag ggaatgatcc tgcgcttgaa
      241 gatgacgacg ttgacgggcg gattgaagcg accgtacttc tttaatgctt aatgcacaat
      301 gcatagtgca tactgagtga acgatcgcaa ttcaataaat tttaggaggt ttagtatggt
      361 ggtaaagaaa gcaacgaaga aagccgcgcc gaagaaaatt gcggcgggca agaacggcag
      421 cagcaacggc aagttcaaat tgacttacgg cacgatgttc aatccaccgg agtcgctgca
      481 taccaatttc gacaaggccg tggcgaaatt ccgcggtgag ctgggcaaag atattcccat
      541 gttcatcaac gggcaggatc gagtcaagat tctgcgcaaa gccgcggcac aaatcgaaaa
      601 acgattgtac gagatcagcg cgattgtgtc gatcgaaatt ggcaagagcc gcatggaagc
      661 gcttggcgac gtgcaagaga ccgccgactt gatttcttac tactgcgatc agatggacgc
      721 caacggtggc tacatcaagc agatgggcaa agatccgctc gtcggttaca acgccaccaa
      781 cttcagcgtg ttgaaaccat acggcgtgtg ggttgtgatc tcgcccttca acttcccgtt
      841 cgccttagcc ggtggcccga cgggcgcggc tttggtcgcg ggcaatgcag ttgtattcaa
      901 gccagcctcc gatacgccgt actccggtcg attgttggcc gaatgctatc gcgatgcagg
      961 tgtgcccgaa ggtgtgttca actacgtgac gggcggcgga tcgacgatcg ggcaagagtt
     1021 gatcgccaac ccgatggtcg atggcatcac cttcaccggc tcatacgatg tgggcatgca
     1081 catctatcac acgtttgcaa cgggcaaata tccgcgaccg tgcatcgccg agatgggcgg
     1141 caagaatccg gccatcgtct cgcgcaatgc cgatttggat ctcgcggtgc aaggcatcgt
     1201 gcgatcggcc ttcggcctgc aaggccagaa gtgctcagcc tgttcgcgcg tgtacgtcga
     1261 taacgcggtc aaagataaat tcgtgcagaa gttagtcgag gccacgaaca aactgaagat
     1321 cggcgatccg acgctgcgcg aaaatttcct cggcccggtg gcaaatcaat cgtcttatca
     1381 acaatatggt gaattcagtg aagagctgtc gcacgacgga cagattctga cgggcggcaa
     1441 gaaattgatc gaaggcgatt acggcaaggg ttatttctgt gcgccgacgc tggccgaact
     1501 gccgacgaat catcgactgt ggaagaccga gatgttcctg ccgatcacga ccgtcgcggg
     1561 ttacgacgac atccatcacg ctatggaatt ggcgaacgat gccgattacg gtttgaccgc
     1621 gggcttctat ggcagccaga aagaactgca atggttcttt gacaacatcg aagcgggcgt
     1681 gacatatgcc aatcggccgc aaggcgcaac gacgggcgcg tggccgggct tccaaccatt
     1741 cggcggctgg aagggctcgg gcagcacggg caaagccggc ggctcgttgt attacgtgcc
     1801 gcagtatatg cacgagcaga gccagacgat tatcgagaag gcgtgatcaa cactatttac
     1861 taatcactat ttattgatca acaattagta aatagtattt agtaagtcaa caactcactc
     1921 agtcaatgtg gaggcgcaga gacgttgaga gattgatcat tctctgcgcc tctgcgtctc
     1981 actttaagac gtttggctcc tcgcgccgtc ctcggcgcga gcaaagcgac catgcgcccg
     2041 ccgaggacgg cgggctgagc aaacaatttc tcaacaagga gcagcatgtc gattcagtac
     2101 atcgttctgg gcgcggggcg acagggaacg gcaagcgcgt acgacttagc caagttcgga
     2161 ggagccgatc gcgtcacgat ggccgatgtc gatcgattcg tggcgcaagc tgcggccgat
     2221 cgagtgaacc agctgatcgg tcgatcgatc gccgatgcga tcgcgctgga cgtgaaagat
     2281 gatcaagcgg tacgccaggc gatcaaaggt tacaatgttg ttttaagtgc cgtgccgtac
     2341 tttttcaatt tgaatctcac caagatcgcg atcgagtgcg gtgtatcgtt ttgcgatctt
     2401 ggtggcaaca cggagatcgt ccgccagcag cacggcctcg acgccgaggc gaagcgcgcg
     2461 ggcgtgacag ttgtgccaga ctgcggcatg gggccgggca tgggcaacac gctcgcggtg
     2521 tatgccatgg agctgctcga caagaccgat cacatttatt tatttgatgg cggcttgccg
     2581 atcgaaccgc aggcaccgtg gaactatgta gaatcattca gcctcgaagg actgaccaac
     2641 gaatatcacg acggtatgat ggtcctgcgc aacagcgagt tgactaccat tccgccgttc
     2701 agcgaaatgg agatcgtgaa cattccgccg ctgggcaaga tggaagcgtt catcgtggcg
     2761 ggcggcgtct cgacaacgcc gtggacgttc caaggcaaac tcaaaacgct gcaattgaaa
     2821 gttctgcgct atcccggcac atttgcgcag ctcaaagcct attcagattt gggcttgtac
     2881 gatttcaagc cgatcaaagt cgacggcgtc gatgtgattc cgcgccgcgt ctttcataca
     2941 ttatatgggc cgcacgttca cgcggatgtc gtcaaggaca tctgcattgt ccgcgcacat
     3001 gccatcggca ggaagaacgg ccagcgcgct gaagcgttga tcgatctggt cgatcgctat
     3061 gatccgcaca ccggcttctc gtcgatggaa cggacaacgg gctggcacct atcgatcatg
     3121 gcggcgatga tcgcgcacaa gcagatcgca gctggcgctg tgccgctgga actggccgtg
     3181 ccgggtcatg cgtttgtggc cgaggctaaa attcgcggct tcaagatcaa aacgaaagtg
     3241 actacggcaa aggcgaaagc aatagcaaag tctagaacaa aatcaaagct acgcaagaac
     3301 taacgagtaa tacgcggtgc ggaaaacgga acccgttgcc ttcggcaagg gcaggcacgc
     3361 accacgcaat cgaacgagga gaccatgcaa cttagaaaat tctttctatt cagtttgatc
     3421 atcgcggcag cgctggccgc gtgcaacaat ccgggaccga tcgacgttag caacccgatc
     3481 gggttcaagc agaagtccgg cttgttcgcg ctgcaggtgc ccaagagctg gacgtcatcg
     3541 caagatgaag tgtccactga agcgatcgct tcgtttgccg ataccacgca ccgcgcagaa
     3601 ttgatcggct atgtgggctt gctcgatcat caactgagtg acgctgaagg cctgcaaata
     3661 gccgcggatt tggccggaaa cttgctcaat caacctggcg acttaaaaat caccgacaag
     3721 taccggcagc ccgatggcgc attcgtcgca gtcttcacct tcacgcgcgc aggcgctcca
     3781 cgaaccggcc aagcgatctt tcgcgatgcc gatctgtctt tggccggcac gttgatcaat
     3841 ggaccggttg acggctgggc ggatcttcaa aaatcactcc agcctttcgt cgattcattc
     3901 aaggtcgata aagatttcat ccaaggcacc tactttacgc cgatcgacac tgacgcatac
     3961 gccgtcgtgg tcccggtcgg ttgggcacaa caaaaatcaa agacgaataa cgaggtcgaa
     4021 gtcaaggcgc ccaatggccg cctgtcgatc atcggtgtgc agctcatagt caccaacacg
     4081 ctcgacgata ctgctttggc aaatcaagcc gtttcaattt tgaaacaagc ctaccgactg
     4141 gacaacacgt tatcggatac cagcaaattg ccggatggcc gcctgcgctt gggacttgat
     4201 cgaaccgatc ggcatacggt cggctacgtc gaacagaagg atggctactt catcggcctg
     4261 ttcttcgatg tgccgacgca gcaactcttg gcctatcagt ctttcatcga ctttgtctat
     4321 tcgacattcg taacgggtaa ggcgaaataa ccggctaacc aagtgaaagg ctgatacggc
     4381 gatcgcatat ccgatcggct gatcaacacg ctaaggatct aacatgtcca aagtcatgtc
     4441 attgcctgat gccattgcca agtttgtcca tgatggcgat acgatttacg cggcgggctt
     4501 cacgcatctg attcccttcg ccgccgggca cgaaattatt cgccaacgca aaaatgattt
     4561 ggtgctggcc cgcgccacgc ccgatctgat ttacgatcaa atggtggcgg cgggctgcgc
     4621 caagaagatc atcttttcat acagcggcaa tcccggcgtc ggatcgctgc gcgtcatccg
     4681 tcaagagatg gaggcggggc gcttggcgta tgaagagtat tcgcactttg cgatgatcac
     4741 gcgcgtagcg gcgggcgcgt cgggtttacc gttcttgcct atgcggccga ccggcgccga
     4801 agacttggcg aaagtgaatc cacaatacaa aacgatcata gatccttata ccggtgttga
     4861 gcttgtaact gtgcctgcgc taaatcccga tgtggcgatc atccacgctc agcgcgccga
     4921 tgcgaatggc aacgcgcaaa tctggggcat cgtcggcgaa caaaaagagg tggcctttgc
     4981 ttcaaagaaa gtgattctca ccgtcgaaga gatcgttgat gagtccgtca tccgatcgga
     5041 tcccaatcgc accttgatac cgggcttcgt cgtcagcgcg gtgtgccacg tgccgttcgc
     5101 cgcgcatcca tcgtacacgc agggctatta cgatcgcgac aacaagtttt acttggaatg
     5161 ggacaagatt agcgagacgc gtgagaacgt gacggcctgg ctggatgaat gggtctatgg
     5221 cttgaaagat cacgcggaat attgggcgaa actcggcagc gaagcacatg atcgattgaa
     5281 ggttcaagct gcgtttagcg agccggtgaa ttacggaaga tactaaacac ataagtgctg
     5341 tcacacccgc tttcgcgagt gtccagatcg caacattttg gattcccgct ttcgcgggaa
     5401 tgactttgcg ctcttcgtgc cttcgcggtt taattttctt attcatggag caatgatcga
     5461 tgaattattc ttcgtccgaa atgatgatcg tcgccgcctc acgcttattg cgcgataacg
     5521 atgtggtctt cgtcggcatc ggccaaccca acctcgcctg caatctcgct cgacgaacgc
     5581 acgcgcctaa tctaaacatg atctatgaag caggcgtcat cggcgcgcaa cccgcgcgtc
     5641 tgccgctatc gatcggcgat ccgtgtttgg tgagcggcgc ggcctcgatc tgctcgatgg
     5701 tcgaaatttt tgcgtactac ttgcagaacg gcaagatcga tgtcggtttc ttgggcggcg
     5761 cgcagatcga tcgatttggc aatgtcaacg ccaccgtgat cggatcgtac gatcatccga
     5821 aagtaagatt acccggcagc ggcggctcag ccgagatcgc cgcgtgggcc agtcgggtgt
     5881 attacatcac accgcatcaa gcccggcgct ttccagaaaa atgtgacttc atcaccagtg
     5941 ccggcttctt agccggtcga tcggatcgcg cagcgaaagg gttgcgcggc agcggtccgc
     6001 aagccgtcgt caccgatctc ggcttgctca cgcccgatcc agattccggc gaattagttt
     6061 tgaccgcgct gcacgcaggc gcgacggtcg agcaagcgat caagaacacc ggttggccgt
     6121 tgaaggtcgc cagcgctttg aaaacaaccg aacccccgac cgctgaagag ctgcgcttgc
     6181 tgcgcgaaga actcgatcca cacggcatgt atctcaagt
//
LOCUS       DASRTJ010000596         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_356716,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000596 DASRTJ010000000
VERSION     DASRTJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356716"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1753
                     /locus_tag="VFF70_12195"
     CDS             119..1753
                     /locus_tag="VFF70_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45500.1"
                     /translation="MNLSDRHSERSEESLPDEIETPRFARGDIKQVRLFIFWAIVALL
                     WVNLPYLIGYASSTSQNRFGGFFIYEQDGNSYLAKMRQGAQGAWNFHLPYTSEDQYQS
                     GGFVYPFYLILGKLETLGFNDQFLYHTARILSSILLLIVLSRFIARFVADRRWQLWTW
                     WLLLFSGGWSLLIGYLIDPKYVAYELIAPDGFIFSILYGPPHVIFGFALLMMWIGYTL
                     DSFQANRSRWPQRILIANLIGGAATLSREAYGAVFVGVLGVFVIARSIQQRRIIWHEL
                     ILITLSSIAAIGYLVYLFIALQTIPGLNIWYQQNPFGSPNLIDFILGFAPLLMVGIIG
                     IALIRRHSSRVANNSSLITLWLIAGPIWAYLPISISRRLIAGWQIPLCIFGAYALMKL
                     WQSRRVIGSILIAAVLPTTVLVIVGGSALVAAKPPTLFQSSDELAALDWLSRNTTDQD
                     VVLSDWRFGNLVPTAADARVFIGHPIETAFFKDKEAEVEHLFDPTTGDIDRRAFLSKW
                     RVTLIVVGTGESIGLGRQAQNIVFRQGAYSIYRFLP"
     gene            1830..3347
                     /locus_tag="VFF70_12200"
     CDS             1830..3347
                     /locus_tag="VFF70_12200"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZY45501.1"
                     /translation="MNIKVVNASIQATAADAIIVNLFEGIAVPAGASGAVDKALSGAI
                     SEVIRANDFRGKLNEVTVIYTRGTIPAARVIVIGLGQVADFSLERIRQAAATAAKKAR
                     ELGCKSIASTAHGAGTGQVDPALAAQAVVEGTLLGLYRWRTNHTEPVDRDQIETFSIV
                     EFDDSKLNAIEAGAQAGEAVALGANRARDLVNQAPNYMTPTSLANAAQAMAKEAGVKC
                     TVHDLEWIKAQKMTAFLAVAQGSIEPPRFIEMEYTGSDAAPIVFVGKGITFDTGGISI
                     KPADQMEDMRSDMGGAAAVIGAIEAIAKLKLSLHVVGLIATCENMPSGSAYRPADVIT
                     ARNGKTIEIISTDAEGRMTLADALDYAADFKPQAVIDLATLTGACVTALGENVAAGYF
                     SNDDALALKVDAASKVSGEKLWRLPLYDEYRDKIKTDYADLKNSGGRYSGVGSSAVFL
                     EEFTQYPWAHWDIAGMALDNTGPTSYYPRIHLPHIQRGGTGFGVRALVAFARNWK"
     gene            3413..5071
                     /locus_tag="VFF70_12205"
     CDS             3413..5071
                     /locus_tag="VFF70_12205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZY45502.1"
                     /translation="MADLSGKPLGQYQLIKPINSGGMATIYTANQPSLDRIVAIKILP
                     DYLIDQPGFLERFATEAHAIAKLDHPHILPIYDYGQADRIPYLVMKHVTGGTLKDLMT
                     GPMDLHQTSVILRQIAEALDFAHKQGIIHRDIKPSNVLMQDSQWVLLADFGLAKIMGD
                     PSQITASGVGVGTPDYMSPEQASGEAVDARSDVYSLGVMLYQMLTGKVPFQADTPLAV
                     MLKHITEPPPSASKSNPKISAEIDHVVQRALAKSPHDRYSNAIELADAFDYALDLNAT
                     LPVQSLASAPRSIGQLWPIGVGAIIVVAIAIVLIGNRNQSAAVIPEVTATPQATATIV
                     PTIALPTSAPTSPPITPNTSQLGDQLFDDFSEDLIDTTRWSYDGAYTATLNSPAVNLQ
                     NGRLAVNAENAADEYLDGELIGQIDNRAFTLVAARVTLLNATGFSDIGLQVNGIDDRP
                     DSWAYLAMNPTDGTISAYIGSDTGTEQTYALEIGKGMPAAHELAIGFKDGQLTFYVDG
                     SPRKSVAALHQGESFWLLFDVEPKSDSNGIISGSFDDVRITYAK"
     gene            complement(5127..5768)
                     /locus_tag="VFF70_12210"
     CDS             complement(5127..5768)
                     /locus_tag="VFF70_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HZY45503.1"
                     /translation="MAKHLILVAGNIGAGKTSLTERLAARLNWQSGFESVADNPYIAD
                     FYKDMRSWSFHLQIFFLGHRALQHKALAEGSQNAIIDRSIYEDMHIFARALHELNNFN
                     ERDYDSYRQLYNVVVDSLPAPALLIYLHAPIEVLVERITKRGRSIESGITPDYLRLLD
                     RYYDEWLTDFSLCPVLKIHSDDLDFVHRNTDLDVVVKKINDRLSGRDEVVFKR"
     gene            complement(5875..6447)
                     /locus_tag="VFF70_12215"
     CDS             complement(5875..6447)
                     /locus_tag="VFF70_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZY45504.1"
                     /translation="MLAIILIALVVVIDQFGFSDRSQRADVIVLLGSMVYPGGKLGPS
                     LERRAEHAVALYQAGLADHIICSGGVGDNPPAEARVACGRVIDLGVPAAAIFYEENSH
                     STEENAAYTAAIMREHGWQTALIDTDGFHLLRATLMFQRAGVVTYPSPAQITAGPMNP
                     IERLAREAREAAGLIYFGAKVALGVDITSR"
     gene            complement(6560..6635)
                     /locus_tag="VFF70_12220"
     tRNA            complement(6560..6635)
                     /locus_tag="VFF70_12220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6600..6602),aa:Gln,seq:ttg)
     gene            complement(6678..7037)
                     /locus_tag="VFF70_12225"
     CDS             complement(6678..7037)
                     /locus_tag="VFF70_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY45505.1"
                     /translation="MMPKLFVITLWAEDVTTCAHFYRDVIGLPLIDVRTDPPHFDLDG
                     AYLVIRKTRSAPLPDREIDRFPIVAFSIDDLDSAIDRLQQHHIDLPREIEADNGGRWI
                     MFYDPGGNLIELTQRTP"
     gene            complement(7107..7223)
                     /gene="rrf"
                     /locus_tag="VFF70_12230"
     rRNA            complement(7107..7223)
                     /gene="rrf"
                     /locus_tag="VFF70_12230"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7292..>7646)
                     /locus_tag="VFF70_12235"
     rRNA            complement(7292..>7646)
                     /locus_tag="VFF70_12235"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1795 a         2072 c         2025 g         1754 t
ORIGIN      
        1 ctcttgtgct gctgccgcat cccagcggcg cgagcgcgtg gaccaacacg cccgcgaacc
       61 aagctctgat ccaaaaagcc attcgagcga tcaaaaaatt gaaacgcgac ctgaatttat
      121 gaacctgtcc gaccgtcatt ccgagcgaag cgaggaatct ctgcccgatg agattgaaac
      181 ccctcgcttc gcgcggggtg acatcaaaca agttcgcctc ttcatcttct gggcaattgt
      241 cgccctcctc tgggtcaatc tgccttacct cattggctac gcctcttcaa cttcacaaaa
      301 tcgcttcggc ggattcttca tctacgagca agatggcaac tcatatctcg ccaagatgcg
      361 gcaaggcgca caaggcgcat ggaattttca tttgccatac acttccgaag atcaatatca
      421 atccggcgga ttcgtttatc cgttttattt gatcctgggc aaacttgaaa cactcggctt
      481 caacgatcaa tttctatatc atactgcgcg aattctaagc agcatcctat tgttgatcgt
      541 gttgtcgcgt ttcatcgccc gcttcgtcgc ggatcgacgc tggcaattgt ggacgtggtg
      601 gttgttgctc tttagcggcg gctggagctt gctgatcggt tacctaatcg atccaaaata
      661 cgtggcctac gaattgatcg cgcccgatgg atttattttc tcgatcttat atggcccgcc
      721 gcatgtgata tttggttttg ctttgttgat gatgtggatc ggctacacgt tggattcatt
      781 tcaagccaat cgatctcgct ggcctcaacg gattctcatt gccaacctca tcggcggagc
      841 ggcaactctg tcacgcgaag cctacggcgc agtcttcgtt ggcgttctcg gcgtgttcgt
      901 cattgctcga tcgatccaac agcggcgcat tatctggcac gaactgatcc tcattacact
      961 ctcatcgatc gcggcgattg gttatctggt ctatcttttc attgcgctgc aaacgattcc
     1021 cggcttgaac atttggtatc aacaaaaccc attcggctca cccaatttaa tcgacttcat
     1081 cttaggcttt gcgccattgc tcatggtagg catcatcggc attgcactca ttcgccgcca
     1141 ttcatcacgc gttgctaata actcatccct cattacacta tggctcatcg ccggaccgat
     1201 ttgggcctat ctgccgatct cgattagccg ccgattgatc gccggttggc agattccgtt
     1261 gtgcatcttc ggcgcatatg cattgatgaa attgtggcaa tcgaggcgag tcattggatc
     1321 gatcttgatc gcagctgtgt tgccgactac ggtcttggtc atcgtcggcg gaagtgcttt
     1381 ggtcgcggcc aaaccgccga cgctgtttca atcgagcgat gaactggccg cgctggattg
     1441 gttgagccgg aatacaactg atcaagatgt ggtgctcagc gattggcgct tcggcaatct
     1501 ggtgccgacg gccgccgatg cacgtgtatt catcggtcat ccgatcgaga cggcgttctt
     1561 caaagacaaa gaggcagagg tggaacatct ctttgatcca actaccggcg atatagatcg
     1621 acgcgctttc ttgtcgaaat ggcgagtgac tttgatcgtc gttgggacag gagaatcgat
     1681 cgggttaggc aggcaggctc aaaatatcgt ctttcggcag ggcgcgtatt cgatttatcg
     1741 atttttgcct taactatcgt gttcccatgc gtgtaatgtt ataattgcac tcaagaaatt
     1801 ggtccacact caataaaact tggagaatca tgaacattaa ggtcgtcaac gcatcgatcc
     1861 aggccactgc ggccgatgcg attattgtga atttgtttga aggcatcgcc gttccggcag
     1921 gcgcgtccgg tgcagtggat aaagctttaa gcggcgcgat ctcggaagtg atccgcgcca
     1981 atgatttccg cggcaagttg aatgaagtca cggtcattta tacgcgcggc acgataccgg
     2041 ctgcgcgcgt gatcgtgatc ggcttaggcc aggtcgcaga cttttcactc gaacgaatcc
     2101 ggcaagccgc cgcgaccgcc gccaagaaag cgcgtgaatt aggctgtaaa tcgatcgcgt
     2161 cgacggcgca cggcgcgggc accggtcaag tcgaccctgc gttggcggca caagcggtcg
     2221 tcgaaggcac gctgttaggc ttgtatcgct ggcgcacaaa tcataccgag ccggtcgatc
     2281 gcgatcagat cgaaacgttt agcatcgtcg aattcgatga cagcaaacta aacgcgattg
     2341 aggcgggcgc acaagcgggt gaagccgtcg cgttgggcgc aaatcgcgcc cgcgacttgg
     2401 tgaatcaagc gccaaattac atgacgccga cgtcattagc aaatgccgct caagcaatgg
     2461 ccaaagaggc cggcgtcaaa tgcacggtgc acgaccttga atggatcaag gcgcagaaga
     2521 tgacggcgtt cctggcggta gcgcagggat cgatcgagcc gccaagattt atcgagatgg
     2581 agtacaccgg atcggacgcc gcgccgatcg tgttcgtcgg caaaggcatc acgttcgata
     2641 caggcggcat cagcatcaaa ccggccgatc aaatggaaga catgcgatcg gatatgggcg
     2701 gagcagccgc cgtcatcggc gcgatcgagg cgattgcgaa attgaaattg tcgctgcatg
     2761 tcgtgggcct gattgcgacc tgcgagaata tgccgagcgg ctcggcttat cgaccggcgg
     2821 atgtgatcac cgcgcggaat ggcaagacga tcgagattat cagcacggac gcggaaggcc
     2881 gcatgacgct ggctgatgcg ttggattacg cggcggattt caagccacag gccgtgatcg
     2941 atctggcgac gctgacaggc gcgtgtgtga cagcattggg cgagaatgtg gccgccggat
     3001 atttttctaa tgatgatgcg ctcgcactga aagttgatgc ggcgagtaaa gtttccggag
     3061 aaaaattgtg gcgcttgccg ctctacgacg agtaccgcga caagatcaag accgattacg
     3121 ccgacctcaa gaacagcggc ggccgctata gcggggtcgg atcgagtgcg gtgtttcttg
     3181 aggagttcac gcaatatccg tgggcgcact gggacatcgc cgggatggcc ttggacaaca
     3241 ccggccccac gtcctattat ccgcgcatcc atctgccgca cattcaacgc ggtggaacag
     3301 gtttcggtgt gcgggcgctg gtggcctttg cacgcaattg gaagtagggg cgagcggtcg
     3361 ttgatcctag taacgaggtg gataggaccg ctcgccctta caattttgat ccatggccga
     3421 tctcagcgga aagcccctcg gacaatatca actcatcaag ccgatcaaca gcggtggcat
     3481 ggcgacgatc tacactgcca accaaccctc actcgatcgg atcgtggcga tcaaaattct
     3541 gcccgattac ttgatcgatc aacccggctt ccttgagcgc ttcgctacgg aagcccacgc
     3601 catcgccaaa ctcgatcatc cgcacattct gcccatttat gattacggtc aggccgatcg
     3661 cataccgtat ctcgtaatga agcacgtcac cggcggcacg ctcaaagatt taatgactgg
     3721 gccgatggat ttgcaccaga cctcggtaat cctcaggcag atcgccgaag cgctcgactt
     3781 tgcgcataag cagggcatca tccaccgcga catcaagccg agcaatgtat tgatgcagga
     3841 cagtcaatgg gtcttgctcg ccgattttgg tctggccaag atcatgggcg atccatctca
     3901 aatcactgcc agcggcgtcg gcgtcggcac acccgactac atgtcacccg aacaagcttc
     3961 gggcgaagca gtcgatgcgc gcagcgacgt gtattcactc ggcgtgatgt tgtatcaaat
     4021 gctcaccggc aaagtgcctt tccaggccga tacgccgctg gccgtcatgc tgaaacacat
     4081 caccgaaccg ccgccctccg cgagcaagag caatcctaag atcagcgccg agatcgatca
     4141 cgtggtgcag cgcgctttgg ctaagagtcc gcacgatcga tatagcaacg ccattgagtt
     4201 ggcggatgcg ttcgattatg cgcttgattt aaacgcgaca ctgcccgtac aatctttagc
     4261 gtctgctccg cgctcgatcg gccaactgtg gccgatcggt gtaggtgcaa ttatcgtcgt
     4321 tgcgatcgcc atcgtcttga tcggcaatcg caatcaaagt gcggccgtca ttccggaagt
     4381 caccgccact cctcaagcaa cagcgacgat cgttccaacg atagcgctgc caaccagcgc
     4441 gccaacttcg ccgcctatta cgcccaacac ctctcaacta ggcgatcagc tcttcgacga
     4501 tttcagcgaa gatttgatcg atacaacccg ctggagctat gacggtgctt acactgctac
     4561 attgaacagc ccggccgtca atcttcaaaa cggacgattg gcagtcaatg cagagaacgc
     4621 tgccgatgaa tatttagatg gtgagctcat tggtcagatc gataatcggg ccttcacctt
     4681 agtcgccgcg cgggtcacgc tgctcaacgc caccggcttc agcgacattg gcttgcaagt
     4741 caacggcatc gatgatcgcc cggactcatg ggcgtacctc gcgatgaatc ccacagatgg
     4801 aaccatatcg gcttacatcg gctcggacac tggcactgag cagacgtatg cactggagat
     4861 tggcaaaggg atgcccgccg cgcatgaact agcgatcggg tttaaggatg ggcagctgac
     4921 tttttacgtg gatgggtcgc cgaggaaaag tgtcgccgca ctgcatcaag gtgaatcgtt
     4981 ttggctgctg ttcgatgtcg aacccaaaag cgattcgaac ggcatcatca gcggcagctt
     5041 cgacgatgtt agaatcacgt atgcgaagta aatgaaccac ggagttcatg gataaaatta
     5101 aaatccgtgt catccgtggt tagtagttac cgcttgaaca ccacttcatc tcgtccgctc
     5161 aatcgatcgt tgatcttctt gacaaccaca tccaaatctg tgttgcgatg tacaaaatct
     5221 aaatcatcac tgtgaatctt aagcacgggg cagagactaa agtcggtcaa ccattcatcg
     5281 taatatcgat cgagtaagcg cagataatcg ggggtgatgc cggactcgat cgatctccca
     5341 cgcttcgtaa tccgctcgac cagcacttcg atcggcgcat gcagatagat caacaatgcg
     5401 ggcgcaggca gtgaatcgac gacgacgttg tacagctgcc gatatgaatc ataatcgcgc
     5461 tcgttgaaat tgtttagttc atgcagcgcg cgagcaaaga tgtgcatgtc ttcgtaaatc
     5521 gatcgatcga taatcgcgtt ttgagagcct tcggccagtg ctttatgctg caacgcgcga
     5581 tggccgagga agaaaatttg cagatggaac gaccacgatc gcatgtcttt gtaaaagtcg
     5641 gcgatatatg gattgtcggc gacggattca aagcccgact gccaattcaa tcgagcggcg
     5701 agccgttcag ttaaactggt cttgcctgcg ccgatgttgc ccgcaacgag aatcagatgc
     5761 ttggccaagg ttcactcctg attgttgatg gctgtcattc tactacacgt agtatgtatt
     5821 gcgtggtgcg tgttgggtga tgcataaaac gctcaacaga acacgcaccg cgattcacct
     5881 tgatgtgata tcaaccccta acgcaacttt cgcgccaaag taaatcaagc ctgctgcttc
     5941 tctcgcttcg cgggcaaggc gctcgatcgg attcatagga ccggcggtga tttgcgccgg
     6001 actgggatac gttacgacgc ctgcacgttg aaacatcaat gtcgctctta aaagatgaaa
     6061 tccgtccgta tcaatgaggg ccgtctgcca gccatgttcg cgcatgatcg cggctgtata
     6121 agcggcgttc tcttcagtgc tgtgtgaatt ctcttcataa aagatcgcag ctgccggcac
     6181 gcccagatcg atcacgcggc cacaggcgac tctcgcttcg gcgggcggat tgtcacctac
     6241 gccgccgcta caaatgatat gatcggctaa tcctgcttgg tataacgcaa cagcatgctc
     6301 ggcgcgccgt tcgagcgacg gaccaagttt tccgccggga tacaccatcg agccgagcag
     6361 cacgattaca tcagcacgtt gagatcgatc ggagaagccg aattgatcga tcacgacgac
     6421 taaggcaatt aaaattatcg ctagcaataa gcacgctatg agaatgaatc gaagcagacg
     6481 cggatgtttg cgcataaata tgcggacagt atatcaagaa aaacggcgtt gaccaattgg
     6541 tcaacgccgc cttcatcttt ggctggggag gaaggactcg aaccttcgaa taacggattc
     6601 aaagtccgtt gccttgccag cttggccact ccccagtgtg caacgatttt atcacgcgtc
     6661 gctataactg acaagggtta cggcgtgcgc tgcgtcaatt caattaggtt gccgccggga
     6721 tcataaaaca tgatccagcg tccgccattg tctgcttcaa tttcccgcgg caggtcaatg
     6781 tgatgctgct gaagccgatc gattgcggag tcgagatcgt cgatggaaaa ggcgacgatc
     6841 gggaagcgat ctatttctcg atcgggcagt ggcgccgatc gggttttgcg gatgaccagg
     6901 taagctccat ccagatcgaa gtgcggcgga tcggtacgga catcgatcaa gggcaggcca
     6961 atcacatcac gatagaagtg cgcacaagtt gtgacgtctt cagcccatag tgtaatgaca
     7021 aacaattttg gcatcatagt aatcctccag aaatataaaa agaccgatac tgaaaattca
     7081 gtatcggtct aattagtaca aaagcctctt ggcaatgacc tactctccca gagggtcgcc
     7141 cctcaagtac catcggcgct agcgggctta acggccgggt tcgggatggg accgggtgta
     7201 cccccgctgc tcaaatcacc aagagactct tgttgaaaaa ctttatcagt tcactgatat
     7261 agaatgcccg agcttcaagt tcaccgcaac acgtgataag ccctcgacca ttaggacagc
     7321 ttagcttaac acattgctgt gcttacacct gctgcctatc aaaccagtag tcttctggcg
     7381 gtctttctcc atttaaggaa gggggatctt atcttggggc gagtttccca cttagatgca
     7441 ttcagcggtt atctctgcca gacgtagcta cccagccatg ccgttggcac gacaactggc
     7501 acactagagg tctgtccact ccggtcctct cgtactaaga gcagctcccc tcaaatcccc
     7561 tacgcccaca gcggatagag accgacctgt ctcacgacgg tctgaaccca gctcgcgtgc
     7621 cgctttaatg ggcgaacagc ccaacc
//
LOCUS       DASRTJ010000597         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_37789,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000597 DASRTJ010000000
VERSION     DASRTJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37789"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFF70_12240"
     CDS             complement(<1..433)
                     /locus_tag="VFF70_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45506.1"
                     /translation="MNNRIRKIGSVSLMTLGLLIVLRTALAQDRSTSISVSNEFRVCN
                     TNGSYSQTIQSAVDAAQPGDVIKVAAGVYTESFPVYDYNLYISKTVHLFGGYTCADWL
                     TRDPSINVVTIRPTTTDMSVIFISGIGGQTDLVTPTIDGFLI"
     gene            625..1461
                     /locus_tag="VFF70_12245"
     CDS             625..1461
                     /locus_tag="VFF70_12245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45507.1"
                     /translation="MNFAIRDLTKYEEFTQVIQVQQKIWGLNNSEGLYAPMLKTIAEN
                     GGTVIAAFDADKMIGFVVGFLGRHTDGRIKLCSQTLGVLPEYRNQGVAATLKWTQRQQ
                     MRLTEIDLITWTYDPIEAPNARLNIRTLGGVARIYKEDVFGENFSALTQGLATDRFVV
                     EWWIKSDRVEQRSKGIAEPIGLSSPIVNRCVGSSGDRRIETIDLNSDSPWIRVEIPND
                     LQSIKKTNLALAKDWRLKMREVSGVYFARGYQVIDFVRAGEVWGPTRASHNWYVLQRG
                     GE"
     gene            1678..>2810
                     /locus_tag="VFF70_12250"
     CDS             1678..>2810
                     /locus_tag="VFF70_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HZY45508.1"
                     /translation="MAFRDLREFIKALEQRGELIRIKTLVDPELEITEITDRISKGPA
                     GQNKALLFENVTGSSMPVLINAFGSAERMALALNVNDLEELNHNLAKTIDLKLPQGLK
                     AGLDRGRDLLGVLKSIGLGPKIVKNAPAQEIVITDQVSLAKLPILKCWPLDGGRYITL
                     PQVITRDPITNIRNVGMYRLQVKDDRSLMIHWQRHKGGAEHERVAAENKKPIIPCAIV
                     LGGDPASMWCASAPLPPNIDEYLLAGYLRGKPVEFVKCVTQSIEAPAEAEIVIEGYID
                     PNEHDLEGPFGDHTGFYTPADRFPILHVTAITHRKDAIYPTTIVGKPPMEDYWMGKAT
                     ERLFLPLLKLFLGEVVDYNMPAEGIFHNMVIVSIKKRFPGQPQ"
BASE COUNT          676 a          692 c          812 g          630 t
ORIGIN      
        1 tgatcaagaa gccatcgatc gtgggcgtga ccagatcggt ttgtccgccg atcccgctga
       61 taaagataac ggacatgtcg gtggtggttg gccggatcgt cacaacgttg atcgatggat
      121 cgcgcgtgag ccaatccgcg caggtgtagc cgccgaaaag atgaacggtc tttgaaatgt
      181 aaagattgta atcgtaaaca ggaaaggatt cggtgtagac gcccgcggcc actttgatca
      241 catcgccggg ctgcgcggca tccaccgcgg attggatcgt ttgtgagtag ctgccgttgg
      301 tattgcaaac gcggaattca tttgagacgc tgatcgaggt cgatcgatcc tgcgcgagcg
      361 cggtgcgcag gacgatcagc aatcccagag tcatcaaact gaccgaaccg atcttccgta
      421 ttcgattgtt catggctgcc cccttgactg cctctattct agatcggatc gatcgagagt
      481 caagtggcaa ttgacgagtg aaaagtgacg agtaacgagt tatgaagaat gcgatgtgtg
      541 ttgcgtggtg cgtaaggcgt gatatgattt gcaccatctg cactgcacaa gcgcagcgcg
      601 gtgcaagtgt ctgcaaggag acttatgaac tttgcaatcc gtgatctaac caaatacgaa
      661 gagttcactc aagtcattca ggtgcaacaa aaaatttggg gcttgaacaa ttccgaaggt
      721 ttgtacgcgc cgatgctgaa gaccattgcc gaaaacggcg gcacggtcat cgctgccttc
      781 gatgcggata aaatgatcgg cttcgtggtg ggcttcctgg gacgacatac ggatggccgc
      841 atcaagttgt gctcgcaaac gctgggcgtg ctgccggagt atcgcaatca aggcgtagcc
      901 gccactttga aatggacgca gcgccagcag atgcggttga ccgaaatcga tctaatcacg
      961 tggacgtatg atccgatcga agcgccgaac gcgcgtttga acattcggac gttgggcggc
     1021 gtggcgcgga tttataagga agatgttttt ggtgaaaact tcagtgcctt gacgcagggc
     1081 ttagccacgg atcgatttgt ggtcgagtgg tggatcaagt ccgatcgggt agagcagcgc
     1141 tcgaagggca tcgccgagcc gatcgggttg agcagcccga tcgtgaatcg ctgcgttggc
     1201 tcaagcggcg atcggcggat cgaaacgatc gatctcaaca gcgacagccc ttggattcga
     1261 gttgaaattc ccaacgatct acaatcgatc aaaaaaacca atttagcttt ggcgaaagac
     1321 tggcggttga agatgcgcga agtttctgga gtatatttcg cgcgcggtta tcaagtgatt
     1381 gattttgtgc gcgcgggcga agtgtggggg ccaacgcggg cgagccacaa ttggtatgtg
     1441 ctgcagcgcg gcggagagta gtgcgcggag gaaatcttag tccattggca ttgaagaatt
     1501 tcaaaaaccg ccagcctctc tcgggaggtt gcttttttct cttgcccact tatcgatcgt
     1561 gtggtaacat ctaattgaca tcacgaacaa acgcgccccg tgaataactg ggcgcgtttt
     1621 tgttgcatgc gacaaccgta gaatcggcat gccgatgccg atggcttgtg aggacacatg
     1681 gcctttcgcg atctgcgtga atttattaaa gccttggaac aacgcggcga attgatccgc
     1741 atcaaaactt tggtcgatcc tgaattagag atcactgaaa ttaccgatcg gatcagcaaa
     1801 ggcccggcgg gtcagaacaa ggcgctgctt tttgaaaatg tgacaggctc gtcgatgccg
     1861 gtgctgatca atgcgtttgg cagtgctgaa cgcatggcgc tggctttgaa cgtgaatgat
     1921 cttgaagagt tgaatcacaa tcttgccaag acgatcgatc tcaaacttcc gcagggcttg
     1981 aaagccgggc tcgatcgagg acgcgatcta ttgggcgtgc tcaaatcgat cgggttgggg
     2041 ccgaagatcg taaagaacgc gcccgcgcaa gagatcgtca tcactgatca ggtttcgctt
     2101 gcgaagttgc ccatcctcaa atgctggccg ctcgatggcg ggcgttacat caccctgccg
     2161 caggttatca cgcgcgatcc catcaccaac attcgcaacg tcggcatgta ccgcttgcaa
     2221 gtcaaagacg atcgatcgtt gatgatccac tggcagcggc acaaaggcgg cgccgaacac
     2281 gaacgtgttg ccgctgaaaa taagaaaccg atcatcccgt gcgcgattgt gctcggtggc
     2341 gatccggcgt cgatgtggtg cgcgtccgcg ccgctgccgc ccaacatcga cgaatattta
     2401 ctggccggtt atttgcgcgg caaaccggtt gagttcgtga agtgtgtgac gcaatcgatc
     2461 gaagcgccag ctgaagccga aattgtgatc gaaggttaca tcgatccaaa tgaacatgat
     2521 ctcgaaggac cattcggcga tcacaccggc ttctacacgc ccgccgatcg attcccgatc
     2581 ttgcacgtca ccgcgatcac tcatcgcaag gacgcaatct atccgacgac gattgtcggc
     2641 aagccgccga tggaagatta ctggatgggc aaagcgacgg aaagattatt cttgccgctg
     2701 ctgaaacttt ttctgggtga ggttgtcgat tacaacatgc cggctgaagg catcttccac
     2761 aacatggtga tcgtgtcgat caagaaacgt ttccccggcc agccgcaaaa
//
LOCUS       DASRTJ010000598         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_281414,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000598 DASRTJ010000000
VERSION     DASRTJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281414"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1554)
                     /locus_tag="VFF70_12255"
     CDS             complement(373..1554)
                     /locus_tag="VFF70_12255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZY45509.1"
                     /translation="MEHILDKVTLGALVLVRDKMMAQQASGRKVYRLEVGDTSFNTPA
                     NIRQAINDAMEQGKTHYPPSTGLPQLRAAMLKKMRDENHLPIKDAEHVMVTIGGMHGL
                     YMAFCALLDPGDEVILPDPMWSEIAELIKRPGGVPVPITLKPDRAFLYDPIEIEKAIT
                     PRTKAIYLNTPHNPIGVVLSEETLKQIADIAIRHNLAVVSDEAYEHVIFDGKKHLSIG
                     SLPGMADRTITSFTFSKTYAMTGLRLGYMASNDELLNDRFRKLLRLTTNGVPSITQWG
                     GVAALTGPQDAVHEMAKELEARRNIFFEGIQSIKAFDSFRPEGAFYVWSKISDKWAGY
                     QGKRDSWAMTNYLIDQAGIGTSPGIAFGPSGEGYVRFAFTVDRATLIESIEVMQDLFN
                     K"
     gene            complement(1980..>2882)
                     /locus_tag="VFF70_12260"
     CDS             complement(1980..>2882)
                     /locus_tag="VFF70_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZY45510.1"
                     /translation="IFVAEGTYTENLTVNIQVELFGSYIVSGSTWLPGTPGASTINGS
                     SSIGQPVVTFGGVADGAILNGFTVTGGSVITDGGGIAISSVSPIIQACIIKGNTGGSP
                     SIEWGSGGILIGNGSAPIISNTIIMSNTGTGGASGIRVGSNVTLTVINSVIAQNTGRF
                     AIHANGPNLIKLINVTIANQVNDGGILLNQGSQAVITNSIIWEPSNQALALADANSVF
                     TVTYSDVSDGVLSGTGNLSIDPKFIGSGNYHLKVGSPVIDKGTSNGAPIADLEGTPRD
                     ARPDMGAYEWVGFRVYLPLALKNY"
BASE COUNT          619 a          833 c          733 g          697 t
ORIGIN      
        1 gaatcgacgg gcatgtggat cgcaccacaa tcattcacgc ccgaccagtc ttcttcgtca
       61 agaacgatcc agatcgccgg agcgatcgac cattgatcta attgtacgtt catgagttgc
      121 ctctatggtt cggcgaaggc aactcgatcg agttaccttg ccactgggca ggtgaggcga
      181 tgcctcagcc tgcggctgat acgataaatt tcggtccttg tatcagtgcc atctttagtc
      241 atgtggcatc acctcctctc tctgtcccgt cattgcgagg agcgctctgc gcgacgaagc
      301 aatctcacct tcggtcgatc ggaagattgc ttcgccgcca gcggcggctc gcaatgcaat
      361 gatgctgcta atttatttgt taaacaggtc ctgcatcact tcgatcgatt cgatcaacgt
      421 cgcgcgatcg acggtgaaag cgaagcgtac gtagccttcg cccgaagggc cgaacgcaat
      481 gcccggcgat gtgccgatgc ccgcttgatc gatcaagtag ttggtcatgg cccatgaatc
      541 acgcttacct tgataacccg cccacttgtc cgaaatcttc gaccagacgt agaacgcgcc
      601 ttcgggccgg aatgaatcaa acgccttgat cgattggatg ccttcaaaga aaatattgcg
      661 ccgcgcttcc agttctttcg ccatctcatg caccgcatct tgcggcccgg tcagcgcggc
      721 gactccgccc cattgcgtga tcgatggcac accattcgtc gtgagtcgca gcaacttgcg
      781 gaatcgatcg ttgagcagtt catcgttgct ggccatgtag cccaagcgca agccggtcat
      841 cgcatacgtt ttcgagaacg tgaacgacgt aatcgtccga tcggccatgc cgggcagcga
      901 gccaatgctc agatgctttt tcccgtcgaa gatcacatgc tcgtaagcct catcgctgac
      961 gaccgccaga ttgtgtcgaa tggcgatgtc ggcgatctgc ttcaacgttt cttcggacag
     1021 cactacgccg atcggattat gcggcgtgtt cagatagatc gccttggtgc gcggcgtgat
     1081 cgctttctcg atctcgatcg gatcgtacag aaaagcccga tcgggtttca gcgtgatcgg
     1141 aaccggcaca ccgcccggcc gcttgatcaa ttcggcaatc tcgctccaca tcggatcggg
     1201 caggatcact tcatcgcccg gatcgagcag cgcgcagaag gccatataca aaccgtgcat
     1261 gccgccgatg gtgaccatga catgctcggc atctttgatc ggcagatgat tctcgtcgcg
     1321 catcttcttc aacatcgctg cgcgcagctg cggcaggcca gtcgacggcg gatagtgcgt
     1381 cttgccttgc tccatcgcat cattgatcgc ctggcgaatg ttggccggcg tgttgaacga
     1441 cgtgtcgccg acttccagtc gataaacttt gcggccggac gcttgctgcg ccatcatctt
     1501 gtcgcgcacc aacaccagcg cgccgagtgt aactttatct aaaatgtgtt ccataagtct
     1561 ccttgatcaa tcctcccgca ggtttattga aatatggaca tttgtcgtga agaacctgcg
     1621 ggaggttcac cacaaatgaa aacgcctctc atccacactg ggacgagagg cgctcgcggt
     1681 tccacccaaa ttcgatcgat cttagatcaa tcgcactctg cgctgtaacg ggctgacccg
     1741 gaatcactta tttgttttgc ggtcatggtg atgaccgctc tacgttctgc gatccacgtg
     1801 tggtagttta ctaccacgag cgggcggttt tcatcgactc ttggcggaga aggctctcaa
     1861 gcggtggcct tcagtccctg tcgcgtcgaa ctcgattact cgtcccgctc aacgtgttga
     1921 ttgttaagtt gttgtgatta tatcgatttt gatgcgattg tcaacccatc aaatttctat
     1981 taatagttct tcaacgccag cggcagatac actcggaagc ccacccactc gtatgcgccc
     2041 atgtccggtc gggcatcacg cggagtgcct tccaagtctg cgatcggcgc gccgtttgat
     2101 gtgcctttat cgatcacggg cgagccgact ttcaaatggt aattgcccga accgataaac
     2161 ttcggatcga tcgagagatt gcccgtgccg cttaacacgc cgtcgctgac atccgaatag
     2221 gtaaccgtga atacactatt ggcatctgcc aacgcaagcg cctggttaga cggctcccag
     2281 atgatgctgt tggtaataac cgcttgagaa ccctggttca gcaatatccc gccgtcgttc
     2341 acttgattag caatggtgac attgatcaac ttgatgagat tcgggccgtt ggcatggatc
     2401 gcaaatcgac cggtattttg ggcaattaca ctattgatca cggtcaacgt cacatttgac
     2461 ccaacgcgaa taccactcgc cccgccggtg ccggtgttac tcatgatgat cgtgttactg
     2521 ataattggcg cactgccgtt gccgatcagg ataccaccac tgccccactc gatcgatggt
     2581 gagccgcccg tattgccctt aatgatacac gcttgaatga tcggtgagac gctactgatt
     2641 gcgatcccgc cgccatccgt gataaccgaa ccgccggtta ccgtaaagcc attcaagatc
     2701 gcgccgtcgg ccacaccccc gaaagttaca accggttgac cgatcgagct acttccattg
     2761 atcgtgctgg caccgggtgt gccgggcaac catgtcgatc cgctaacgat gtaactgccg
     2821 aataactcaa cttggatatt caccgttaga ttttcagtgt aagttccctc agccacgaag
     2881 at
//
LOCUS       DASRTJ010000599         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_112876,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000599 DASRTJ010000000
VERSION     DASRTJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112876"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFF70_12265"
     CDS             <1..881
                     /locus_tag="VFF70_12265"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013308751.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZY45511.1"
                     /translation="PEMFKKSYSNVFGENAQWNEIPITGGELYEWNEESTYIQEPPYF
                     DGLTLDVKPIQHISDLRVLAIFGDSVTTDHISPAGNIAESSPAGLYLKSRGVEKKDFN
                     QYGARRGNDRVMTRGTFANPRIKNLMVPGVEGSVTIKYPENNQMTIYDAAMAYKAEGR
                     SLFVIAGKEYGSGSSRDWAAKGPYLQGVRAVIVESFERIHRSNLVGMGILPLQFKEGQ
                     NAASLGLTGKEVIHITGLDDNLKPRQDVAVKAMKDDGTSIEFKATVRIDTPIEVEYYR
                     NGGILHTVLRKLVKEG"
     gene            complement(1079..1564)
                     /locus_tag="VFF70_12270"
     CDS             complement(1079..1564)
                     /locus_tag="VFF70_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZY45512.1"
                     /translation="MLINDKIAAAFNQQVGYELGNSNQYIAVAAYFEGQSLMGLAKMF
                     FKQADEEREHAMKFVKFILDAGGKVVVPAIGAVQNDFKSAEDAAQLALDAEVRTTNQI
                     NDLVTLSIAEKNYTAQNFLQWFVNEQLEEVSSAQTHLDVIKRAGSNVLMVEAYLAHID
                     A"
     gene            1854..2066
                     /locus_tag="VFF70_12275"
     CDS             1854..2066
                     /locus_tag="VFF70_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45513.1"
                     /translation="MAIKVKNAKKIIEPGTRYINDVRMGIEMEQGYPMSVAQDMLLRK
                     LATEGKTTVRMAVDKLIEIRPRPQFR"
     gene            2156..>3241
                     /locus_tag="VFF70_12280"
     CDS             2156..>3241
                     /locus_tag="VFF70_12280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZY45514.1"
                     /translation="MIKDTPYWWDTAPIFPDHSNRPIPEKVDVIVIGSGYTGLSTARS
                     LAKNGAKVIVLEKETIGWGASSRNGGQVLTGLKVGVSDLIKKFGLERARELYNSSLAS
                     IDFTEQLIAAEQIDCEYERSGHIDAAYKPKHFDHFKRTAEILARDFNRPVKLVARSDQ
                     HSELGTHYYHGLMIDESSGQLHPAKYVRGLALAVERYGVELHEKTPALKIEKEQSGFR
                     ITTPRGSIATKEVVVATNGYTDQSAQKIRRRVFPVGSYIIATEPLRSDVAAKILPNQR
                     VVFDSKNFLYYFRLSSDNRMLFGGRAQFTPSTPNSTHTSAEILRRGMVEVFPELADVH
                     VEYVWSGNVCFTLDKFPRSGCIDGIYYA"
BASE COUNT          800 a          812 c          895 g          734 t
ORIGIN      
        1 agccggaaat gttcaagaag tcgtatagca atgtgtttgg cgagaacgcg cagtggaatg
       61 aaattccgat cacgggcggc gaactctatg aatggaatga agagtctacc tacattcaag
      121 agccaccgta cttcgatgga ctgacgctcg atgtgaagcc gattcaacac atcagcgatc
      181 tgcgcgtact ggcaatcttc ggcgattccg tgaccaccga tcatatttcg cccgccggta
      241 atattgccga gagcagtccg gccggattgt atttgaaatc gcgcggcgtt gagaagaaag
      301 acttcaatca atacggcgcg cggcgtggca acgatcgagt gatgacgcgc ggcacgttcg
      361 ccaatccccg tatcaagaat ttgatggtgc ccggcgtcga aggcagcgtg acgatcaagt
      421 atcctgaaaa caatcagatg acaatctatg acgcggcgat ggcctacaaa gccgaaggtc
      481 gatcactgtt cgtgattgcg ggcaaagagt acggtagcgg ctcgagccgc gactgggcgg
      541 cgaaaggccc gtatctgcaa ggtgtcagag cggtcatcgt cgaatcgttc gagcgaattc
      601 accgcagcaa cttggtgggt atgggcattc tgccgctgca attcaaagag ggacagaacg
      661 cggcctcgct gggtttaacg ggcaaagaag tgattcacat cacgggcttg gatgacaatc
      721 tcaagccgcg ccaagatgtc gccgtaaaag cgatgaagga cgatggaacg agtattgagt
      781 tcaaggcaac ggtgcgcatc gatacgccga tcgaggtgga gtattatcgg aacggcggga
      841 ttttgcatac agtgctgagg aagttagtca aagagggcta accgtagagc acgcaaggaa
      901 cgcagagata aaataataac gggactcgat tagattgatc gagtcccatc gctattcata
      961 attatgcatg tcattcccgc ggaagcggga atccagattt acttgactgg acacccgctt
     1021 ttggtacacc ctgaggggat ttaccaccga atggcgcggg tgtgaccgtt tgaggtcgtt
     1081 atgcgtcgat gtgcgccaag tacgcttcga ccatcaacac gttcgatccg gcgcgcttga
     1141 tcacatccaa gtgcgtttgc gcagacgaga cttcttccag ttgttcattg acgaaccact
     1201 gcaagaagtt ctgcgcggtg tagttcttct cggcgatcga cagcgtgaca agatcgttga
     1261 tctggttcgt cgtgcggact tccgcatcga gcgccagctg cgcggcgtct tcggccgatt
     1321 tgaaatcgtt ctgcacggcg ccgatcgccg gcacgacgac tttgccgcct gcgtccaaaa
     1381 tgaactttac aaacttcatg gcgtgttcgc gttcttcatc ggcttgcttg aagaacatct
     1441 tggccagacc catcaagctc tgtccttcga agtaggcggc aacggcgatg tactgattcg
     1501 aattaccgag ttcgtagccg acttgctgat tgaacgcggc ggcgatcttg tcattgatca
     1561 acatggttca tgctccttaa ggaaattttg gaatgacttt gctcattacg ctttacgatc
     1621 agctgtcatt tcgagcgctg tttgcgagaa atctacacga tcattagcaa ggcttgttca
     1681 gcggggcaga gatttctcac ttcgttcgaa atgacaaacg aatgtcttgt tcaattataa
     1741 cggacagtca gccgatcgag tagcgcggaa tgtcgatgaa aaacatgaca cttgtctagg
     1801 ccttgccgca aaatcgatta taatggacgc atcagacgcg cagtgaggtt gacatggcca
     1861 ttaaagttaa gaacgccaag aaaattatcg agccgggcac gcgctatatc aatgacgtgc
     1921 gcatgggcat tgaaatggag cagggttatc cgatgtcggt tgcacaggat atgctgctgc
     1981 gcaagttggc gacagaaggt aagaccactg tgcggatggc cgtcgataaa ctgatcgaga
     2041 ttcggcctcg gcctcaattc cgctagagcc gcccgttcca agcgaccggc gcctgaatcg
     2101 atcgggtggc cggtcttttt ctaactttta acttccaaca tccacaggaa tactcatgat
     2161 taaagacacg ccctactggt gggacaccgc gccgattttc cccgatcact ctaatcgacc
     2221 aattccggag aaagttgatg tcattgtaat cggcagcggg tacaccggac tatcgaccgc
     2281 gcgcagcttg gcaaagaatg gcgcgaaggt gatcgtgctg gaaaaggaaa cgatcggttg
     2341 gggcgcgagt tcacgcaatg gcggccaagt cttgaccggc ctgaaagttg gcgtaagcga
     2401 cttgatcaaa aaatttggac ttgaacgcgc gcgtgaactt tataattctt cgttggcctc
     2461 gatcgatttt acggaacagc tgatcgccgc tgagcagatc gattgcgaat acgagcggtc
     2521 gggtcacatc gatgcggcct acaaaccgaa gcacttcgat cacttcaaac gcacggcaga
     2581 aatcttagcc cgcgacttca atcgtccagt caagctggtg gctcgatcgg atcagcatag
     2641 cgaactcggc acccactatt atcacggctt gatgatcgat gagagcagcg gccaactgca
     2701 tccggctaaa tatgtgcgcg gcctggcgtt ggctgtcgag cgatatggcg tggaacttca
     2761 tgagaaaacg cccgcactca aaatcgagaa agaacaatcg ggttttagga tcaccacacc
     2821 gcgcggatcg atcgcgacga aggaggttgt ggtcgctacg aatggctaca cggatcaatc
     2881 ggcacaaaag atccggcggc gagtgtttcc cgtcggttcg tacatcatcg ccaccgagcc
     2941 gctgcgatcg gacgtggccg ccaaaatttt gccgaaccag cgagttgtct tcgattccaa
     3001 aaactttttg tattactttc gcctatcatc agataatcgc atgttgttcg gcggccgtgc
     3061 gcagttcaca ccctcgacgc cgaacagcac acacaccagc gccgaaattt tgcggcgcgg
     3121 catggtcgaa gtgtttcctg aattggccga tgttcatgtc gaatatgttt ggagcggcaa
     3181 tgtatgcttc acgctcgata agttcccacg cagtggctgc atcgatggca tctattatgc
     3241 g
//
LOCUS       DASRTJ010000600         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_113130,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000600 DASRTJ010000000
VERSION     DASRTJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113130"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFF70_12285"
     CDS             <1..1074
                     /locus_tag="VFF70_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HZY45515.1"
                     /translation="ILAPFMRPGSKPMAEIVYRHYQLERGLKVVAIGGGTGLSIFLRG
                     IKAHTSNITAIITVADDGGSSGRLRRELGVPPPGDFRNCLAALADDESLTAQLFQYRF
                     AQGEELDGHAFGNLFITAMADVSGSFEQALIESSRVLAITGRILPSTLNDVSISAEIK
                     TDQGLVRVDGESAITLTRGSIERVILKPANARPYPDTIRSILDADMIVLGPGSLYTSV
                     LPNLLINSIPDAIRASKALTLYICNVATQHGETDRYSVLDHAHAIELHIGPNVIDAII
                     ANSHTNIEWHNPPEGVGEMIQVEASSTIPIINADVADESRPWRHDSQKLARAVMNAFA
                     ALRIPERGNVSKRSSTQPAEVPR"
     gene            1143..2471
                     /locus_tag="VFF70_12290"
     CDS             1143..2471
                     /locus_tag="VFF70_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45516.1"
                     /translation="MPVTRREFLRFIGLSGVTLALPGSCDALVVKLIAALQPTPTLVP
                     IATIPPTSTATLSPTNTPAPTAAPASTTTPRSILTSLLPYYAKDARNNLVVRNGQRAM
                     LSSHVYPKATFVRDSFYGPLALGDAPLGYDCYRWFEQTQDQASGQIRTAVPFLPANEP
                     LFPSQDDDSSLLFIPWSMWLKRHGLPIDQALVEKAFAFVQTHVHDDWFVSPAGPFRYW
                     ADTVAPDVDERITHNQGLYVLALRSMIEFGWGGVTDRDLTNARSNYAACYKSDIGAIT
                     LGKDSWWADKLDISVLFPEFMLRWLFGEAALSDDVIRSTTDRFIQKASVFNNNEIAGI
                     KVICASDGSFLPPEKYFASVLNRPGDYQNGGYWPMYTLIALALRYKIDPDSALKQMIE
                     QLVQNELARDHRSKEVMMLIPGEVGNIDPNRSGYTWNALIAPALKWANVV"
     gene            2632..>3646
                     /gene="gap"
                     /locus_tag="VFF70_12295"
     CDS             2632..>3646
                     /gene="gap"
                     /locus_tag="VFF70_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZY45517.1"
                     /translation="MTVKVGINGFGRIGRQVLKAIRDYYPNTLEVVAFNDIGDLKTMA
                     HLLKYDSNYGRFNGTVAVQGEDTLLVDGKPIKVFKETDPSKLPWGDLGVDIVIESTGL
                     FTIKKDGVNKKGKTVLGAENHITKGGAKKVIISAPAEGEDLTVVLGVNDDKYDNKIHH
                     VISNASCTTNCLAPAAKVVHDKFKIARGLMTTIHAYTNDQRILDLPHSDLRRARAAAL
                     SIIPTTTGAAKAIALVIPDLKGKFDGYALRVPTSTVSVVDFTAQVEVPTTTDELRQAF
                     RDAAAGPMKGILAAVDEPLVSIDYKGDPHSSSVDLPFTMVLGKEKNDFLKVVTWYDNE
                     WGYS"
BASE COUNT          859 a         1027 c          945 g          815 t
ORIGIN      
        1 atcctggcac cgtttatgcg tcccggcagt aagcccatgg ccgagattgt ttatcggcat
       61 tatcagctcg agcgcggctt gaaagttgtg gcgattggcg gcggcactgg cttatcgatt
      121 tttttgcgcg gcatcaaagc ccacacgtcc aacatcacgg cgatcatcac cgttgccgat
      181 gacggcggat cgagcggacg gctgcgccgc gaactgggtg tgccgccgcc cggcgatttt
      241 cgcaactgcc tcgccgcatt agccgatgat gaatccttga cggcgcagtt gttccaatat
      301 cgattcgcgc agggcgaaga actcgatggt cacgcttttg gaaatctgtt catcaccgcc
      361 atggcggatg tcagcggatc gttcgagcaa gcgctgatcg aaagcagccg cgtgctggcg
      421 atcaccggac ggattttacc gagcacgctg aacgatgttt cgatcagtgc ggagatcaaa
      481 accgatcaag gtctggtgcg tgtcgatggc gaatcggcga tcaccctcac ccgaggatcg
      541 atcgaacggg tgattttgaa accggccaat gcccgcccgt accccgacac aatccggtcg
      601 atcctcgacg ccgatatgat cgtgctgggt ccgggcagtt tatacaccag cgtgctgccc
      661 aacttattaa tcaacagcat cccggatgcg atccgcgcca gcaaggcgct gaccttgtat
      721 atttgcaatg tcgccacgca gcatggcgaa accgatcgat attccgtgct cgatcacgcg
      781 cacgcgatcg agctacatat cggcccaaat gtgatcgacg cgatcatcgc taactcgcac
      841 acgaacatcg agtggcacaa tccacccgaa ggcgttggcg aaatgatcca agtcgaagcc
      901 tcatcgacta ttccgatcat caacgccgat gtcgccgacg aaagccggcc gtggcggcac
      961 gattcacaaa agttggcacg cgctgtgatg aacgcctttg cagctttgcg cataccggag
     1021 cgcggcaacg tttcaaaacg atcatcgact caaccggcag aagttccaag ataaaccaac
     1081 aagtcgcaca tcgatccgct tcttcgactt caagtcactt ccctcaggat gtttttgctc
     1141 acatgcccgt cactcgtcgc gaatttttaa gattcatcgg attatctgga gttacattgg
     1201 ccttgcccgg ttcgtgcgat gctttggtcg tgaaacttat cgctgccctt cagccaacgc
     1261 caaccctcgt gccaattgca acaattcctc caacatcgac ggcaacgctt tcaccaacta
     1321 atactcctgc gccaacagct gcgcctgcct cgaccacaac accgcgatcg atcttgactt
     1381 cgctactgcc ttattacgcc aaagatgcac gcaacaacct cgtcgtgcga aatgggcagc
     1441 gcgccatgtt atcgagtcac gtgtatccca aagccacctt cgtgcgcgat tcattttatg
     1501 gaccattggc cttaggtgat gcgccgctcg gctacgactg ctatcgctgg ttcgagcaaa
     1561 cacaagatca agctagcggg caaattcgca ccgccgttcc gttcctgcca gcgaacgaac
     1621 cgttattccc atcgcaagac gatgattcgt cactcctgtt tattccgtgg tcgatgtggc
     1681 tgaagcgcca cggcttgccg atcgatcaag cgctagttga gaaagcgttc gcctttgtgc
     1741 agacccacgt ccatgacgat tggtttgtct cgcccgccgg tccatttcgc tattgggccg
     1801 atacggttgc gcccgatgtc gatgagcgca tcacgcacaa tcaagggctg tatgttctgg
     1861 ccttgcgatc gatgatcgaa ttcggttggg gcggcgtgac cgatcgagat ttaactaacg
     1921 ctcgatcgaa ctacgccgcc tgctacaaat ctgacattgg tgcaattaca ctgggtaaag
     1981 attcctggtg ggccgacaag ctggatatat ccgttttgtt tccggagttc atgctgcgct
     2041 ggttatttgg cgaagccgct ttgtctgatg atgtgatccg ctcaacgacc gatcgattta
     2101 ttcagaaggc cagcgtcttc aataacaacg aaattgcagg tatcaaagtg atctgcgcca
     2161 gcgatggatc gtttctgccc ccggaaaaat attttgcatc cgtgctcaac cggccgggcg
     2221 attatcaaaa cggtggttac tggccgatgt acacgctaat tgcgctggcg ctgcgctaca
     2281 agatcgaccc tgattccgcg ctcaaacaaa tgatcgagca gctcgtccag aacgaacttg
     2341 cacgcgatca tcgttcgaag gaagtgatga tgctgatacc cggcgaggtc ggcaatatcg
     2401 atccgaaccg atcgggctac acctggaatg cgttgatcgc accggcgttg aaatgggcaa
     2461 acgttgtttg aggctttaca ccaaaattcc gcttaagatc aaattgaagt cacctcatcg
     2521 atcttacttt cctcactggt aatccactgc aagacgagtt aaaatgatga ggttgaaacg
     2581 attcatcgcc ctcaagcgat gatacattta tacgaataag gagatgtaac aatgacagtc
     2641 aaagttggca tcaatggatt tggccgcatc ggacggcagg tgctcaaagc aattcgagac
     2701 tattatccga atacattgga agtcgtcgcc ttcaacgaca tcggcgacct caaaacgatg
     2761 gcgcatctgt tgaagtacga ctcgaactac ggccgcttca atggcacggt cgccgtgcag
     2821 ggtgaggata ccttgctggt cgacggcaaa ccgatcaagg tgttcaagga aactgatccg
     2881 tcgaaactgc cttggggcga tctgggtgtc gatatcgtca tcgagagcac agggctgttc
     2941 acgatcaaga aagatggcgt gaacaagaaa ggcaagaccg tgctcggcgc agagaatcac
     3001 atcaccaaag gtggtgccaa gaaagttatc atctccgctc cagctgaagg cgaggacctc
     3061 acggtggtgc tcggtgtgaa tgacgataag tacgacaaca agattcatca cgtcatctcg
     3121 aatgcatcct gcactaccaa ctgtctcgca ccggcggcaa aagttgtaca tgacaaattc
     3181 aaaatcgcgc gcggcttgat gacgacgatt cacgcctaca ctaacgatca gcgcatcctc
     3241 gatctgccgc acagcgatct tcgccgcgcc cgcgccgccg ccctcagcat catcccgacg
     3301 acgacgggtg ccgccaaggc aatcgctttg gtgattcccg atctcaaggg caagttcgat
     3361 gggtatgcgc tgcgtgtgcc gacctcgacc gtatcagtcg tcgattttac cgcgcaagtc
     3421 gaagtgccca ccaccaccga tgagctgcgc caggccttcc gtgatgcagc tgccggcccg
     3481 atgaaaggca ttctggccgc tgtcgatgag ccgctggtca gcatcgacta taaaggtgat
     3541 ccacacagtt cttcggtcga tttgcccttc acgatggtgc tcggcaaaga gaagaatgac
     3601 tttttgaaag tcgtcacctg gtacgacaac gaatggggct atagtg
//
LOCUS       DASRTJ010000601         4709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_129588,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000601 DASRTJ010000000
VERSION     DASRTJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129588"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFF70_12300"
     CDS             complement(<1..732)
                     /locus_tag="VFF70_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZY45518.1"
                     /translation="MSQILFGQSYYLRFDPKLFAALQPYPPLGTLYAASYLRQRGYDV
                     ALFDAMLAESEDEWAAALDRHRPQFAVLYEDNFNYLSKMCLLRMREAAFTMIDLAKAR
                     GCTVILCGADVTDHASLYFEHGADFALIGEGEETLGELIDRLSGKAVTPFEDILGLAY
                     RNAEFGLRNQDTAITNPQSALKMNPRRPDITHLDDLPFPAWDLVDIPRYQKIWRERHS
                     YYSMNMVTTRGCPYHCNWCAKPIWGQ"
     gene            complement(825..1742)
                     /locus_tag="VFF70_12305"
     CDS             complement(825..1742)
                     /locus_tag="VFF70_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45519.1"
                     /translation="MSSLVGDQRIVIEQAILKTVLYSDLFDYPLTPDEITHYLIEVPA
                     DADLVRDRLCDPIWLDGQIARVDHFIMARGRESIVARRLDRSRTSDRLWPRARRFVRV
                     LAALPFVRMVGITGALAMDNSAADDDIDVMIVTATHRVWLTRALSVALVYIGRIFGDT
                     LCPNYMISESALLLETQTLFTAHEFMQMVPLYGFEVYVRMRQLNDWIDAVLPNAARPF
                     RSEPEVRSGLIGRSVKRFGECLLSGRLGDRLEAWEMNRKMKKFQSQMHEIESNAILDQ
                     DHVKGHFKDYGAPVTRLYESKLKEFQLVE"
     gene            1849..2343
                     /locus_tag="VFF70_12310"
     CDS             1849..2343
                     /locus_tag="VFF70_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45520.1"
                     /translation="MSSRFSLGSLTRQQRLVLGAALLTNVAILGILIWLVFSSTIPTI
                     APPPIEPNNAIECENNAALSLRQQGVSGSIMISGSESIQVYINGADAGAAWNVFSTTA
                     TLLNYGCGPYRVVRVDVPDPDQRSNTRLRLEVSWLDVQAWANDLIDDGQLSERMRRQV
                     YQTQ"
     gene            complement(2420..2599)
                     /locus_tag="VFF70_12315"
     CDS             complement(2420..2599)
                     /locus_tag="VFF70_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45521.1"
                     /translation="MTAQAIDLENLQVDPQIEIDPSNPRSDDTYCWVCGASIVDVHCK
                     IVCQHCGFMRDCSDP"
     gene            2721..3875
                     /locus_tag="VFF70_12320"
     CDS             2721..3875
                     /locus_tag="VFF70_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZY45522.1"
                     /translation="MIFNRQHMEDFEEQTLAPYGMRSRNSRGRVYPEQEHPYRTAYQR
                     DRDRIIHTTAFRRLEYKTQVFVVTEGDYYRTRLTHTLEVAQIGRTLARALGANEDLAE
                     TICLVHDIGHPPFGHSGEQALNQLMKDHGGFDHNQQALRIVQYLEQRYPEWPGLNLTY
                     ETREGIVKHETEYDTQSYAEEFEPHKRGLLEAQIANAADEIAYNAHDLDDGLRAGLFT
                     PADLDQLELWQQLKHSVGWDGREFNDVWRHRLIRRLIGLEVTDVVNATDERLTAAKII
                     SIEDLQKQPKNVIGFSDELKQINRGLKDFLFKQMYRHYRVTRMQIKADRLLSELFTAY
                     VGEPGQMPEAARKRIDQDGLQRAVCNYLGGMTDRFALDEHAKMFDPQVRV"
     gene            complement(4013..>4709)
                     /locus_tag="VFF70_12325"
     CDS             complement(4013..>4709)
                     /locus_tag="VFF70_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45523.1"
                     /translation="AGTGGTSPLLALVGLAVIAIIAIALLIIILQRLRSRQMLPPEEP
                     ITLPGQLSGIGPQTGASVAVAPAPNLARGAPKPVVPTQWAGESQPPLAQYVTQYALGD
                     DRYDMSFSIEQGNGDFLGECGVGISETIGVGNPDKVTALEVWLFDKNDIRTLTKVFMS
                     EHCFNDPSLRAKLAPKGEAVMIKKGEVVTLTTQTLKVNARVIDLQYGTGNLPENSFFQ
                     QAKIELAAWSMGS"
BASE COUNT         1125 a         1273 c         1237 g         1074 t
ORIGIN      
        1 ctgcccccag atcggtttgg cgcaccagtt gcaatgatac gggcagccgc gcgtcgtgac
       61 catgttcatc gaatagtagc tgtgccgttc gcgccaaatt ttttgatagc gtggaatgtc
      121 taccaaatcc cacgcgggaa atggcaaatc atctaagtgc gtgatgtccg gtcgacgtgg
      181 attcattttg agtgcggatt gcggattggt gattgcggtg tcctggttcc gcaatccgaa
      241 ctccgcattt cgatatgcta gaccgagaat atcctcaaat ggcgtaaccg ctttgccgct
      301 taaccgatcg atcaactccc ctaatgtttc ttcgccctca ccgatcaacg cgaagtctgc
      361 gccatgctca aagtatagac tcgcgtgatc cgtcacatcc gctccgcata gtatcaccgt
      421 acagccccgc gccttcgcca gatcgatcat cgtgaaggcc gcttcacgca tgcgcagcaa
      481 acacatcttg ctcaaataat tgaagttgtc ttcatataag acagcaaact gcgggcgatg
      541 ccgatcaagc gccgcagccc attcgtcttc ggattcagcc agcattgcat cgaacaacgc
      601 cacatcatag ccgcgctgcc gcaggtaaga cgcagcatac aacgtgccca acggcggata
      661 cggctgcagc gctgcgaata acttcgggtc gaagcgcaga taataggatt ggccgaatag
      721 aatttgactc atagatgatt ggtgagattg cttcgtcgct tcattcgact gcgcagcgat
      781 aggatgccac tccgcccagg atgctccccg caatgatgcg tgcttcattc gactaactga
      841 aattctttta gcttgctctc atacaatcgc gtgacgggcg cgccgtaatc tttgaagtgc
      901 cctttgacgt gatcttgatc gagaatggca ttactctcga tctcgtgcat ctgcgactgg
      961 aactttttca ttttgcgatt catttcccag gcttctaatc gatctccaag gcggcctgac
     1021 aacaaacatt cgccgaagcg ttttacgctg cggccgataa gacccgatcg aacttcgggt
     1081 tctgatcgaa acggtcgagc ggcgttaggt aatactgcat cgatccagtc attcaattgg
     1141 cgcatgcgaa cgtatacctc aaaaccatat aacggcacca tttgcatgaa ttcatgcgcc
     1201 gtaaacaaag tttgcgtctc caataacagc gcgctctctg aaatcatgta gttgggacac
     1261 aacgtatcgc caaatatcct gccgatatag accagcgcaa cgctcaacgc ccgtgttagc
     1321 cagactcgat gagtcgccgt gacaatcatg acatcaatgt catcatcggc cgcgctgttg
     1381 tccatcgcca gagcgcccgt gatgccaacc atacgcacga acggcaatgc ggccagcacg
     1441 cgcacaaatc tgcgagctcg cggccataac cgatcggacg tgcgagatcg atcgagccgg
     1501 cgagccacga tcgattcgcg gccacgcgcc atgatgaagt gatcgacacg cgcgatttga
     1561 ccatccagcc agatcggatc acacaatcga tcgcgcacaa ggtccgcatc agccggaact
     1621 tcgatcaagt aatgcgtgat ttcgtcgggt gtcagaggat agtcaaatag atcggaatac
     1681 aagaccgtct tgagaattgc ttgttcgatg acaattcgtt gatccccgac gagagacgac
     1741 atacgacttt tctcaatctc gagtttgagt tatggtgtta gtacgcagct acgctgggca
     1801 cggattgttt tacttgatgt gcccgttcaa gcatgttatg atcgggtcat gtcatcacgc
     1861 tttagtttgg gctcgttaac ccgccaacag cgcttggtct tgggcgcagc cttattgacc
     1921 aacgtcgcta tattaggaat actgatctgg ttagtgtttt cttcaacgat acccacgata
     1981 gcgccgccgc cgatcgagcc taacaatgcg atcgagtgcg agaacaacgc tgcgttgagt
     2041 ttacgccaac aaggcgtttc tggatcaatc atgatcagcg gatcagaatc gatccaagtg
     2101 tatatcaatg gcgcggacgc cggcgcagcc tggaatgtat tcagcacaac cgcaactttg
     2161 ttgaattacg gctgcgggcc atatcgagtc gtgcgcgtcg atgtgcccga tccggatcag
     2221 cggtccaaca ctcgcttgag gctggaagtc agctggctgg atgtgcaagc ctgggctaat
     2281 gacttgatcg atgacgggca attatcagag cgtatgcggc ggcaagtgta tcagacccaa
     2341 tagccccacc tcggccctcc cctgacaatt cactcacatc gattatgata gaaattctcg
     2401 aattgtcagg agagggagac tatggatcgc tgcaatcgcg catgaaaccg cagtgctggc
     2461 aaacgatctt gcaatgcacg tcgacgatgg acgctccgca cacccagcag taagtatcat
     2521 cgctgcgcgg atttgatgga tcgatttcga tctgcggatc aacttgcaag ttttccagat
     2581 cgatcgcctg agctgtcatg aattaaatgt tagcatagtt tcaatcaata tgtaaggggt
     2641 taaaaccttg cgagcgattg agggtcgaat gattaccact caattcaacc ctcgcaaggt
     2701 taacgaagat tggaggctaa atgatattca accgtcaaca catggaagac tttgaagagc
     2761 aaacgctggc cccgtatggc atgcgcagcc gcaattcgcg cggccgtgtt tatcccgaac
     2821 aggaacatcc ctatcgaacc gcataccagc gcgatcgcga tcgcatcatt catacgaccg
     2881 cttttcgacg gctcgaatac aagacgcaag tcttcgtcgt gaccgaaggc gattattacc
     2941 ggacccgatt gacccacacg ctcgaagtgg cccagatcgg ccggacgctg gcgcgggcgt
     3001 taggcgcgaa tgaagacctg gccgagacga tctgcttagt gcatgacatc ggccatccac
     3061 cgttcggcca ttcaggcgag caggcgctca atcagttgat gaaagatcac ggcggcttcg
     3121 atcacaatca gcaggcgctg cgcatcgttc aatatttgga gcagcgttat ccggagtggc
     3181 ccggcctcaa cctgacttac gaaacacgcg agggtatcgt caagcatgag accgaatatg
     3241 atacccagag ttacgctgaa gaatttgagc cgcacaagcg cggcttgctg gaggcgcaga
     3301 tcgccaacgc ggccgacgag attgcgtata acgcgcatga ccttgacgac ggactgcgcg
     3361 ctggcttgtt cacgcccgct gatttggatc aactcgaatt gtggcagcaa cttaaacaca
     3421 gcgtcggctg ggatggacgg gaattcaatg atgtttggcg gcatcgattg atccgccgcc
     3481 tgatcgggct ggaagtgaca gatgtggtca acgcgaccga tgaacgttta accgccgcga
     3541 agatcatatc gattgaggat ttacagaagc agcccaaaaa tgtgatcggt tttagtgatg
     3601 aattgaagca gatcaaccgg ggcttaaaag attttctctt caagcagatg tatcggcatt
     3661 atcgggtgac gcgcatgcag atcaaggccg atcgattgtt gagcgagttg ttcactgcct
     3721 acgtgggcga accgggacaa atgccggagg ccgcccgcaa gcggatcgat caagacggct
     3781 tgcagcgcgc ggtctgcaat tacctcgggg gcatgacgga tcgatttgcg ctggatgaac
     3841 atgccaagat gttcgatccg caggtgcgag tgtaagcccc acccccatgc caacttttga
     3901 ggattatttg cctatcactt aatttgagtt gtccggtgag gggtgcagat gtgaggaata
     3961 aaaacgcggc gcactgtgta atcgtgcgcc gcgttttttt atgcgttgag agttagctgc
     4021 ccatgctcca cgcggccaac tcaatctttg cttgctggaa gaaactattc tccggtaagt
     4081 tgcccgtgcc atattgtaga tcgatcacgc gcgcattgac tttcagggtt tgggttgtca
     4141 acgtcaccac ctcgcccttc ttgatcatca ccgcttcacc cttgggcgcg agtttcgccc
     4201 ggagtgatgg atcgttgaag cagtgctcac tcatgaagac tttggtcaac gtgcgaatgt
     4261 cgttcttatc gaacaaccac acttcgagcg cggtaacttt atcgggattg ccgacaccga
     4321 tcgtttcgct gatccccacg ccgcattcgc ccagaaaatc accattgccc tgctcgatgc
     4381 tgaaggacat atcgtatcga tcatccccca acgcatactg cgtcacgtat tgagccagcg
     4441 gcggttgtga ttcgcccgcc cattgagtag gcactaccgg tttgggcgcg ccacgggcca
     4501 gattgggcgc gggcgcaact gcaacacttg cgccagtctg tggaccgatg ccgctgagct
     4561 ggccgggaag cgtaatcggc tcttccggcg gcagcatttg gcgactgcgc aagcgctgta
     4621 aaatgataat caacagcgca atcgcgatga tggcaatcac cgctagtcca accaaagcca
     4681 gcagaggaga agtgccgccc gtgcctgca
//
LOCUS       DASRTJ010000602         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_281936,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000602 DASRTJ010000000
VERSION     DASRTJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281936"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFF70_12330"
     CDS             <1..332
                     /locus_tag="VFF70_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45524.1"
                     /translation="ITERNQTIEGLANVLEWLINGAYSMPRSEVFGLNARGIESQIFA
                     REIDVEASPIALLGHGTWVIAQVEIDPDKGSEITPIGKLSLPIRFHRALKVYRAGPRS
                     NFEKMLD"
     gene            473..1729
                     /locus_tag="VFF70_12335"
     CDS             473..1729
                     /locus_tag="VFF70_12335"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZY45525.1"
                     /translation="MAFLSELLGKPVTDLDGNRLGNLDDVIAGIRGELPHPALVAIVV
                     KRSHGQLLIPFSDVAVLIAPAIPLNRQLANIVPYQPAENDLFLARDVLDKQIIDTNGV
                     RVVRVNDLELTRIGGSFYLANVDVGGLGLLRRLGLAKTAERFVNRLGRSSAVGMISWH
                     QVEMLPGDQPMRLRVPGDKLSELHPADLAEIISDLSPAESDKLLESLDVKTLADTLEE
                     VEPDFQASLVEDMSDERVADVLEEMSPDEAADLLAELPEDRSHDLLNLMQRDEANDVR
                     KLLTYHEESAGGIMNTEFIAMQPDLTAEQAISVLRETAAEAETIYYIYVIDDDQHLNG
                     VFSLRELVLAKPDTPITQFMHRRVITVGLQDSQEEVAQAISKYNLVAVPVVDDQNRLH
                     GIVTADDALDKIIPTAWKKRLPRLYH"
BASE COUNT          413 a          498 c          520 g          412 t
ORIGIN      
        1 tcatcacgga gcgcaatcaa acgatcgagg gcctagcgaa tgtgctggag tggctgatca
       61 atggcgccta tagcatgcct cgatcggaag tgttcggcct gaacgcgcgt ggaattgagt
      121 cacaaatttt tgcgcgcgag atcgatgtcg aagcctcgcc gattgcgttg ttgggccacg
      181 gcacgtgggt gatcgctcaa gtcgagatcg atccggataa agggagtgag atcacaccga
      241 tcgggaaact gagcctgccc attcggtttc accgagccct caaagtttat cgcgccgggc
      301 cgcgatcgaa cttcgaaaaa atgttggact aaggacgagc acatcgctcg tccttacttt
      361 ttctccgcaa aaatgttgtt acttggttga cactcacgat catcggtgct aagattccgc
      421 ggcgctgctg aaatgagcca gtcatttcaa gatttcgcgc cgagagtaac gtatggcttt
      481 tctcagcgaa ttgcttggca agccggtgac ggatctggat ggtaaccgtc ttgggaattt
      541 agatgatgtg atcgctggca ttcggggcga actgccgcat cctgcgctgg tcgcgatcgt
      601 tgtcaaacga tcgcatggtc aactcctcat ccctttctcc gatgtagccg ttctcattgc
      661 gcctgctatt cccctcaatc gacagctggc caacatcgta ccgtatcaac cggctgaaaa
      721 cgatcttttc ctggcgcgcg atgtgctgga caagcagatc atcgatacga acggtgtgcg
      781 ggtggtccgc gtgaatgatc tggaactcac ccgcatcggc ggcagtttct atctggcgaa
      841 tgtcgatgtt ggcggcttag gcttgctgcg ccgattaggg ttagcgaaaa cggccgagcg
      901 gttcgtcaat cgcctgggcc gatcgagcgc agtgggcatg atctcctggc atcaagtgga
      961 aatgctgccc ggcgatcagc cgatgcgtct gcgtgtgccg ggcgacaagc tttccgaatt
     1021 gcacccggcc gatctggccg agattatcag cgatctctca ccagcggaaa gcgacaaact
     1081 gcttgaatcg ctggacgtga agacgctggc cgataccttg gaagaagtgg agcccgactt
     1141 tcaagccagc ctcgtcgaag atatgtccga cgagcgcgtg gccgatgtgc tcgaagaaat
     1201 gtcgccggat gaagcggcgg acttgctggc ggaattgccc gaagatcgca gtcatgatct
     1261 gctgaacctc atgcagcgcg atgaagccaa tgatgtccgc aagctgttga cttatcatga
     1321 agagtctgcg ggcggcatca tgaacaccga attcatcgcc atgcagcccg atctcaccgc
     1381 cgagcaggcg atttctgttt tgcgtgagac ggccgccgag gctgaaacga tctactacat
     1441 ttacgtgatc gatgacgacc agcatttgaa cggtgtgttt tcactgcgcg agctggtgct
     1501 ggccaagccg gatacgccga tcacccagtt catgcaccgc cgcgtgatca cggtcgggtt
     1561 gcaggacagc caggaagaag tggctcaggc gatctcgaaa tataatttgg tggctgtgcc
     1621 ggtcgtcgac gatcaaaatc gattgcatgg cattgtcacc gccgacgatg cgctcgataa
     1681 gattattccc acggcgtgga agaaacggct gccacgactg taccactaaa cgacgaccaa
     1741 agaccaataa ccatcattga ttttcgatcg ctcactcttc actcacagct gcactgcacc
     1801 gcacgcaatg cggtacacgt gtcacttatc acgatcccat gac
//
LOCUS       DASRTJ010000603         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_357359,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000603 DASRTJ010000000
VERSION     DASRTJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357359"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3047)
                     /locus_tag="VFF70_12340"
     CDS             complement(<1..3047)
                     /locus_tag="VFF70_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HZY45526.1"
                     /translation="MRCQISRSISLWVVFSIFLTLLAACNSTQPEPQLDVAAAAKATL
                     DARATLEAAVKATLDARQPVTGDAKMWTVMLYEDGDDPILEQDIMTDFNEMERAGSTD
                     QVNIVAQVDRYKGGYKGMGDWSTAKRFYVTQDNDMNSIHSQEIDDLGEVNMSDGNSLT
                     DFIVWAATNYPAQHYALVMSDHGAGWPGGFSDKDNGGIGADKIVLATDVFGGDNLWLM
                     EINRALTAARQQTGIDKFDLIGFDACLMAQIEVFAMLQQHAKFAVASEETEPTLGWAY
                     TNFLRALDRTPTMDGGQLANAVVDAYIDQDQLIVDDAQRKLFVKREFDFEGDTTAAEV
                     ADLKKKDVTLAAIDLAAIPNVLTALDDFAVKLSQINQNDAAQARAYAQSYESVFGTDV
                     PRSYLDLDNFAQVAAQISGDSHVADSANTLSAAIHQAVIDERHGPKRPGSSGMAIYFP
                     IPALFNLEDNLGYVDVANEFSAQSQWPNFLAFHAASGAQSENFNGSGGNNPSRASTIR
                     SSQKPLDVAPIKLSAEVARPDQPVTITSKVTGDRLAYLFTFVGRVLPKEQVLLVEDED
                     FLLADSTKSVDGVKYPDWSTDGVAIDYNWDPIVYTISDGKNAVKALIAPEEYDQSPVY
                     AVEGTYKFVDGSPVIRARLFFQDAKLTKILGYSGQGAQGSLHEITTAPGDQFTVLEQG
                     FTITDTAEPQVHEQDGGTLTFGTQQFTIETAPAASGSYVVGIIAQDLDGNFFPQFESV
                     FVDNKATTSIEGFKPYANETLGFALLYPGQWTVEENLPNQNVLFSSNDANTVVSVARL
                     SFPDAATTADADAQGLQTARDLLSGTQDFQTVTEEQDFLLGGYNGRTQDFTLTYQGQE
                     FYGSIVVATPKTGTTVGFIIIDLKDDFNNQLDNFNSMLNSFDILLSGLSKQETGPALT
                     GFQAITFKDTLSNSAGGLDTSSGDWGQSSYTSNGEFSYELKPGQGPIYDYYADKQLPQ
                     NFALQATASYTGTNDNGYGVIFRVVADGKFYVFRISGDGFYTV"
     gene            complement(3365..>4015)
                     /locus_tag="VFF70_12345"
     CDS             complement(3365..>4015)
                     /locus_tag="VFF70_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HZY45527.1"
                     /translation="LEFYVLETKSYASSLKINERGEFSFWDDYRRRYVDIESPIEQNR
                     RHVIVLEKILKDKGLMPTRFAMLMTPTFKPYVLVANKSRIDRPDTKQFNTDMVIKADA
                     LFRQIEANFDAMGISAMAKLVSSQTVQELGQRLILYHTPIEFDYYAKFGVEPVSPTSS
                     SPVASPSQPKTSDIPTSKYYCFKCKTPIDEKVAYFCRTHKERFGGRLYCREHQKDF"
BASE COUNT          890 a         1057 c         1104 g          964 t
ORIGIN      
        1 tcgacggtat agaagccgtc gccggaaatg cggaagacgt agaacttgcc gtccgccacg
       61 acgcggaaga tcacaccata gccgttgtca tttgtgccgg tgtaactggc tgtcgcttgc
      121 agcgcaaagt tctgcggcag ctgcttgtcc gcgtagtaat cgtagattgg gccttgaccg
      181 ggcttgagct catagctaaa ctcgccgttc gaggtgtagc tgctttgacc ccaatcgccg
      241 ctgctggtgt ccaagccacc ggccgaatta ctaagcgtat ctttgaacgt gatcgcctgg
      301 aaaccggtca gggccgggcc ggtctcttgt ttgctcaagc cggagagcag aatgtcaaag
      361 ctattcagca tcgaattgaa gttgtccagt tgattattga aatcatcttt caagtcgatg
      421 ataatgaagc cgacggtcgt gccggtcttg ggcgtagcca cgacgatcga tccgtaaaat
      481 tcctggcctt gataggtgag cgtgaagtct tgggtccggc cattgtaccc gccgagcagg
      541 aaatcttgct cttccgtcac ggtttgaaag tcttgcgtgc cgcttaagag atcacgcgca
      601 gtttgcaagc cctgcgcatc cgcatcggct gttgtggccg catccgggaa actcaagcgc
      661 gcgacactca ccacggtatt ggcatcgttg gaactgaaga gcacattctg attgggcaga
      721 ttctcttcaa cggtccattg accggggtac aacagcgcaa agcccagtgt ttcattggca
      781 tacggcttga agccttcgat cgaagtcgtc gctttattat cgacgaagac actctcaaac
      841 tgcggaaaga agttgccgtc caaatcttgc gcgatgatgc cgacgacgta actgccgctg
      901 gccgcgggcg cagtctcgat ggtgaattgc tgcgtgccga aggtgagtgt gccgccgtct
      961 tgttcgtgga cttgcggttc agcagtatcc gtaatcgtga agccctgttc taagaccgta
     1021 aattgatcgc cgggcgcggt ggtgatttca tgcagcgagc cctgtgcgcc ttgaccggag
     1081 tagcccagaa tcttggtgag ttttgcatct tgaaagaata agcgcgcgcg gatcaccgga
     1141 ctgccgtcca caaatttgta cgtgccttcg acggcataca ccggcgattg atcgtactct
     1201 tccggcgcga tgagtgcctt aaccgcattt tttccgtcgc tgatcgtgta cacgattgga
     1261 tcccagttgt aatcgatcgc tacgccgtca gtggaccaat cgggatactt caccccatcg
     1321 acggacttgg tgctgtcggc cagcagaaaa tcttcatctt cgaccagcag cacttgctct
     1381 ttaggcagca cgcggccgac aaacgtaaac aaataagcca ggcgatcgcc ggtgactttt
     1441 gatgtgatcg tcacgggctg atcgggccgc gcgacttccg ccgagagttt aatcggcgca
     1501 acatcgagcg gcttctgcga tgatcgaatc gtgctggcgc gcgatggatt gttgccgccg
     1561 ctgccgttga agttttccga ctgcgcaccg ctggcagcat ggaaggccag gaagttaggc
     1621 cactggcttt gtgccgaaaa ttcattggcg acgtcgacgt accctaaatt gtcttcgagg
     1681 ttaaacaaag ccggaatcgg gaaatagatg gccatgccgg aagagccggg ccgcttgggg
     1741 ccgtgccgtt cgtcgatgac ggcttgatga atggccgccg acaaggtatt cgcgctgtcg
     1801 gcgacatgac tatcgccgct gatctgcgcg gctacctgcg caaagttatc cagatcaaga
     1861 tacgagcgcg gcacatccgt gccgaacacg ctttcatagg actgggcata ggcgcgcgct
     1921 tgcgcggcat cgttttgatt aatctgcgac agcttcaccg caaaatcgtc gagcgcggtt
     1981 agaacattcg ggatcgccgc tagatcgatc gcggccagcg tgacatcttt ctttttgaga
     2041 tcggccactt cggccgccgt ggtgtcgcct tcaaaatcga attcgcgctt gacgaataat
     2101 ttgcgctggg catcatcgac gatcaattga tcttgatcga tataagcatc caccacggca
     2161 ttggccagct gaccaccgtc catggtggga gtgcgatcga gggcgcgcag gaagttcgta
     2221 tacgcccagc ccagcgtcgg ttcggtctct tccgaagcga ccgcgaattt ggcgtgttgc
     2281 tgcagcatgg cgaacacttc gatctgcgcc atgaggcacg catcaaaacc gatcaaatca
     2341 aacttgtcga tgccggtctg ctggcgagcg gccgtcaacg cccgattgat ctccatcagc
     2401 cacaagttgt cgccgccgaa gacatctgtg gccaggacaa tcttgtcggc gccgatcccg
     2461 ccgttgtctt tatcgctgaa accgcccggc cagcctgcgc cgtgatcgga catgaccagc
     2521 gcataatgtt gggcgggata atttgtggcg gcccacacga tgaaatccgt tagcgaattg
     2581 ccgtcggaca tattgacttc gccaaggtca tcgatctcct gcgagtggat cgaattcatg
     2641 tcattatctt gcgtgacata gaagcgtttg gcggtgctcc agtcgcccat gcctttgtag
     2701 ccgcccttgt agcgatcgac ctgcgcgacg atgttaactt gatcggtgga gccggcgcgt
     2761 tccatctcgt tgaagtcggt catgatgtcc tgctccaaga tcggatcgtc gccgtcttcg
     2821 tacagcatca ctgtccacat tttggcgtcg ccggtgactg gctgtcgcgc atcgagtgtc
     2881 gctttgacgg cggcttccag cgtggcacgc gcatcgagcg ttgccttcgc agcagcggcg
     2941 acatcgagct gcggttcagg ctgcgttgaa ttgcacgcgg ccagcagcgt caagaagata
     3001 ctgaagacaa cccataagct aatcgatcga gaaatttgac agcgcatgct gcttcctcct
     3061 accggcgatg attttgatca ttctacatga ttggggacgg agtgacaaga taaaaaagat
     3121 tgtggaatgc ggagtgcgga aggcggagtc attagccacc gtcattgcga gcgccgttac
     3181 cctgcaaact gaaggcaggg tcttcggggc gcgtggcaat ctcattaggt tgctgtaaac
     3241 ccataggaga gtgttatgaa attcgttcag cgattgaaat cgcggcaacg acaacgctaa
     3301 accgaccttc gtcggctggg ggcagtctct ggatcgacac ataggttgat ccctaccctg
     3361 cggattaaaa atccttttga tgctcacggc agtacagcct gccgccaaag cgctctttat
     3421 gcgtccgaca gaaataggct actttctcgt cgataggagt tttgcacttg aaacaataat
     3481 actttgaggt tggaatatcc gatgtttttg gttgcgatgg agaagcaact ggcgaggatg
     3541 aagttgggct aacaggttct acgccaaact tggcatagta atcaaattcg attggcgtgt
     3601 gatagagaat taaacgctgg ccaagttctt ggaccgtttg gctggagacc aatttcgcca
     3661 tagcgctaat gcccatcgca tcgaagttgg cttcaatctg acgaaacaaa gcgtcggctt
     3721 tgatcaccat atcggtgttg aattgtttag tatctggccg atcaattctc gacttatttg
     3781 ccactaacac ataaggcttg aatgttggag tcatcagcat ggcaaagcgc gttggcatca
     3841 agcctttatc cttcagtatt ttctctaata caataacatg ccgcctattc tgctcaatag
     3901 gtgactcgat gtcaacatag cgccgccgat aatcatccca aaaggaaaat tcaccgcgtt
     3961 cgttgatttt caaactagaa gcataactct ttgtttccaa gacatagaat tccaa
//
LOCUS       DASRTJ010000604         1925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_357809,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000604 DASRTJ010000000
VERSION     DASRTJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357809"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /gene="tatA"
                     /locus_tag="VFF70_12350"
     CDS             complement(<1..125)
                     /gene="tatA"
                     /locus_tag="VFF70_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZY45528.1"
                     /translation="MAGFGATELIIILVIILLLFGVGRIATVGKELGTGMREFRKG"
     gene            complement(229..402)
                     /locus_tag="VFF70_12355"
     CDS             complement(229..402)
                     /locus_tag="VFF70_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45529.1"
                     /translation="MIALSAEALHAKAESIAQYRSQLSTFFKDEAEIDTRLKAYAQEV
                     GGGKLVERYWRKV"
     gene            complement(439..570)
                     /locus_tag="VFF70_12360"
     CDS             complement(439..570)
                     /locus_tag="VFF70_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45530.1"
                     /translation="MKIFVPLTAGQHVDHLIARATAEHVNQPLIYSEDFPYAENSTK"
     gene            complement(779..1006)
                     /locus_tag="VFF70_12365"
     CDS             complement(779..1006)
                     /locus_tag="VFF70_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZY45531.1"
                     /translation="MKMPKISRTIFLSPHPDDAALSCGGTISQLSKNDQHLGVITLFG
                     GNRSAATPLSDFARGLHARWQLTDEAPAAPR"
     gene            1044..1115
                     /locus_tag="VFF70_12370"
     tRNA            1044..1115
                     /locus_tag="VFF70_12370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1076..1078,aa:Lys,seq:ttt)
     gene            complement(1176..1763)
                     /locus_tag="VFF70_12375"
     CDS             complement(1176..1763)
                     /locus_tag="VFF70_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZY45532.1"
                     /translation="MQIKNPPQALLADLISGDDIRAERAALLVAASGEAALEALRQLV
                     TDPNADHRWWAARTLSQIRSAASIQELLPLLADQNADVRACAVYALGELQASSAVETI
                     AKLLIDSSVFVSAMASDALARIGRSSVPILIERLKQGESLERARAARALLAILDSRSI
                     PALIAALDDDSPVVEHYASEALTRLGIGTILVKPQ"
BASE COUNT          422 a          497 c          534 g          472 t
ORIGIN      
        1 cccttgcgga actcgcgcat gccggtgcct aattccttgc ccacggtcgc aattcgtcca
       61 acgccaaata gtaatagaat gatcaccaag atgatgatca attcagttgc accaaatcca
      121 gccatagggt cgatacctcc tgggccgaag cccgtattgt tgatcggatt atagcacagt
      181 cgccgcttca atgggcgacg cacttataaa atgcgtcgcc catttcattc atacttttcg
      241 ccaatatcgc tcgactaatt tcccgccgcc gacttcttgt gcatatgcct taagacgtgt
      301 gtcgatctca gcctcgtcct tgaagaaggt gctgagctgc gatcgatact gcgcgattga
      361 ttcggccttg gcgtgcagcg cctcggcgct tagtgcgatc aattttggct gccatttatc
      421 agcgtcccag acaggttgct attttgtact gttctccgca tatggaaagt cttcggaata
      481 gatcaaaggt tgatttacgt gttcagccgt tgcgcgcgcg atcagatgat cgacgtgctg
      541 gccggcagtg agcggaacga aaattttcac ggtcgatccc gagctttgga tcgccgcgat
      601 cttcgatcgg agagctgcag ccacccgacc ggtgatctct gcttcgcgga tcgatccgaa
      661 gattgcagcc tcagaatcat ataacgattg gagcgtgatc ggatcgacgc gatagataca
      721 atccgcaaac ggcagttgaa ggagaaaggc agccagacga tcgagtgcgg cacgatcttc
      781 atcgcggtgc ggcaggtgcc tcgtccgtta actgccagcg cgcatgcaag ccgcgagcga
      841 aatccgataa tggcgtggca gctgatcgat tgccgccgaa gagcgtgatc actcccaggt
      901 gttgatcgtt cttcgatagt tgagagatcg ttccgccgca agacaaggcg gcgtcatcgg
      961 gatggggcga taagaagatt gtccgcgaaa tttttggcat tttcattgac agtccacggt
     1021 cggcatgtta caatcttggg cgcgggccgt tagctcaatg gtagagcaga ggccttttaa
     1081 gcctttggtt cagggttcga gcccctgacg gctcatcaaa atgttcgatg gttgaaagac
     1141 catcgaacat tttttatcgc gttcgtttct gttttttatt gcggcttcac caagatcgtg
     1201 ccgatgccta aacgggtgag tgcttcagaa gcgtaatgtt caaccacagg gctgtcatca
     1261 tcgagcgcgg caatcagggc ggggatcgat cgggagtcga gaatcgccag cagcgctctg
     1321 gccgcgcgcg cgcgttccag tgactcgccc tgcttcaagc gctcgatcaa gatcggcaca
     1381 ctggatcgac caatgcgggc taacgcgtct gaggccattg cactgacgaa cacactggag
     1441 tctatcagta attttgcgat agtttcaaca gcgctactgg cctgcagttc tcctagcgca
     1501 tatactgcgc atgctcgaac atcggcattc tgatcggcaa gcagcgggag taattcttga
     1561 atggaggcag ccgatcggat ttgactgagg gtgcgcgctg cccaccagcg gtgatcggca
     1621 ttgggatcag tgacgagctg ccgaagtgcc tcaagcgctg cttcaccgga cgcggctaca
     1681 agtaacgcgg cgcgttcggc gcgaatatca tcgccactga tcaaatcggc gagtaacgct
     1741 tgaggtgggt tttttatttg catcaattgg gcggccgata aaataaaccg gttgatgggt
     1801 taggctcaac cggattgcat tatcaacatc tctcgtttcg ctggaaggcc gatcgggatt
     1861 tagctagggc tgagccagtt tttcgatcgg tgtatttttg tacttgtcgc cttcttcgat
     1921 caaca
//
LOCUS       DASRTJ010000605         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_357823,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000605 DASRTJ010000000
VERSION     DASRTJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357823"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1145)
                     /locus_tag="VFF70_12380"
     CDS             complement(168..1145)
                     /locus_tag="VFF70_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZY45533.1"
                     /translation="MVKLRWGLLSTARINRAVIPPIRSAKRSELAAVASRNQAKADAY
                     AKEWKIPRAYGSYEALLADKEIDAIYIPLPNDMHAEWSIKCAEAGKHVLVEKPIALTP
                     GEVDRMIDAAKKNKVVIAEAFMYKHHPQTAQVLDLVKQGAIGEVKLIKGAFTFNLTRT
                     SDVRLVPEQGGGSIWDVGCYPISYARLVAQAEPIEVFGWQVTGSTGVDEVFSGQMKFA
                     NGLLAQFDCGFRSPFRASLEIVGATGSIELASPFKPDGSTKFVINRGDKIENFQSANQ
                     PLYLGEIEDMENAAIDHQPQRISLEWSRGNVAVITALLESPQIDKVVKL"
     gene            complement(1259..2176)
                     /gene="lipA"
                     /locus_tag="VFF70_12385"
     CDS             complement(1259..2176)
                     /gene="lipA"
                     /locus_tag="VFF70_12385"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZY45534.1"
                     /translation="MILVESIEVSDKPKRRPEWLKVRAPQGETYDELKRLMRVKTLHT
                     VCEEAMCPNIGECWGRGTATFLMMGDVCTRSCGFCDIKTGRPLPLDWDEPNRVAQAIK
                     AMNLRHAVITSVNRDERKDGGAPIFALVIRRVREIQPGCTIEVLIPDFKGNSEALKMV
                     TDAAPEILNHNVETIPRLFKKVQPQDNYEWAMTTLGNAKQLSPTQVTKSGIMLGLGET
                     KEEVIAVMRDLTDRKVDILTLGQYLQPSKQHLPIDRYVPLEEFAELKQIGLELGFRWV
                     ESGPLVRSSYNADKQARALVSGSMFPNSL"
     gene            complement(2252..>2954)
                     /locus_tag="VFF70_12390"
     CDS             complement(2252..>2954)
                     /locus_tag="VFF70_12390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45535.1"
                     /translation="DNADEIERAVSSVLANRGQHSIEIVLIDNGSSDGTAEIVKQIAA
                     THPEVQALWIDHWIGEAAGRNVGLMRSQGRIIIMLGNNVEIAGDIFELIDQALNDPEV
                     GVCGGWGVRSGDLRNFESSEGPDVDAVEAYLMAFRRSSLKRVGLLNEKYRFYRHFDLD
                     FSLRFSDLGQKVIIIPEVRERTKQHVHRIWESTPPDERDRKSKRNFYRFLHRWGDRRD
                     LLLQPAPIHYHADDE"
BASE COUNT          684 a          887 c          688 g          695 t
ORIGIN      
        1 gagcagttat acgggcgcga ttaacaatcc accgagcgta ttattttggg ttaccgaacc
       61 gatttggaaa aacagcggcg acaaagccat cctcagcgac gagcacaacc agaacatcag
      121 cacaaattgc tacggcgaca agtgcccata acaggcgcaa cttttttcta taacttgacc
      181 actttgtcta tctgcggcga ttcaagtaac gcagtgataa cggcgacgtt gccgcggctc
      241 cactccagtg agatgcgctg tggttgatga tcgatcgccg cgttttccat atcttcaatc
      301 tcacccagat acaatggctg attggccgat tgaaaattct caattttatc gccacggtta
      361 atcacaaact tcgtgctgcc atctggcttg aagggactcg ccaactcgat cgatccagtc
      421 gccccgacga tctccagcga tgcgcgaaat ggcgatcgaa agccacaatc aaattgtgcc
      481 aacaagccat tggcaaattt catttgcccg ctaaacactt cgtccacgcc ggtcgatcca
      541 gtcacttgcc agccaaacac ctcgatcggt tctgcctgcg ctacgagccg cgcataactg
      601 atcggataac agcccacgtc ccagatcgat ccaccgccct gctccggcac gagccgcaca
      661 tcgctggttc gagtcaaatt gaaagtgaat gctcctttga tcaatttcac ttcacctatt
      721 gcgccttgct tgaccaagtc caacacttga gccgtttgcg gatgatgctt gtacatgaac
      781 gcttcggcga ttaccacctt gtttttcttc gccgcatcga tcatgcgatc aacttcaccc
      841 ggcgttaacg cgatgggctt ctcgactaac acatgcttac ccgcttccgc acatttgatc
      901 gaccattcgg cgtgcatgtc gtttggcaac ggaatataga tcgcgtcgat ctctttatcc
      961 gccagcagcg cctcatacga gccataggcg cgtggaatct tccactcttt tgcgtacgca
     1021 tcagccttcg cttgattgcg gctggcgaca gccgctaact ctgatcgttt cgccgatcgg
     1081 atcggcggaa tcacggctcg attgattcgg gcggtactca acaatcccca acgaagttta
     1141 accatgaggt catgctccaa tacaagtcat atttaccgca gagaacgcaa agagcgcaga
     1201 gagaaaggca ataataattg ttggcgatct ctgcgctctc cgcggttagt tgaattgtct
     1261 ataggctgtt cggaaacatc gaacctgaaa ccagcgcccg cgcctgctta tcagcgttgt
     1321 aagacgatcg aaccagcggg ccgctttcca cccagcggaa acccaactca agcccaatct
     1381 gtttcaactc ggcaaattct tcgagcggta catagcgatc gatcggcaga tgctgtttgc
     1441 tgggctgcaa atattgaccg agcgtgagaa tatccacttt gcgatcagtc agatcgcgca
     1501 tcacagcgat cacttcttct ttcgtctcgc caagccccaa catgatgccg ctcttggtga
     1561 cttgagtggg actgagctgc ttggcattgc ccagcgtcgt catcgcccat tcgtaattat
     1621 cttgcggttg aaccttcttg aataaacgcg gaatcgtttc gacattgtga ttcaaaattt
     1681 ccggcgcggc gtcggtcacc atcttcaacg cctcgctgtt gcccttgaaa tctggaatca
     1741 acacttcgat cgtgcagccg ggctggatct cgcgcacacg ccgaatcacc agcgcgaaga
     1801 tcggcgcgcc gccgtctttg cgctcatcgc gattcaccga agtgatcacc gcatgccgca
     1861 agttcatcgc cttgattgcc tgggcgacgc gattcggctc atcccaatcg agtggcagtg
     1921 gccgcccggt cttgatatca cagaacccgc acgatcgagt gcagacatcg cccatcatca
     1981 ggaacgtcgc cgtgccgcgt ccccagcatt cgccgatgtt cggacacatc gcttcttcgc
     2041 acaccgtgtg cagcgtcttg acgcgcatca gacgcttgag ttcgtcatac gtctcgccct
     2101 gcggcgcgcg cactttcaac cactccggtc ggcgcttggg tttgtcggaa acttcaatcg
     2161 attcaacaag aatcacatta tccaagatga aactccaatc caaaataaca acgcagtaat
     2221 tctacgctat tcgactgatt cccgcaaagt gtcattcgtc atcagcatgg tagtgaatcg
     2281 gcgcaggctg caacaataaa tcacgtcgat cgccccagcg gtgcaagaag cggtagaagt
     2341 tgcgcttgct cttgcgatcg cgctcatcgg gcggcgtcga ttcccagatg cgatgtacgt
     2401 gctgtttggt tcgctcacgc acttcaggga tgatgatcac tttctgtccc aagtctgaaa
     2461 agcgcagact aaaatcaaga tcgaagtggc gatagaagcg atatttctcg ttgagcaagc
     2521 ccacgcgttt aagcgaggat cgacggaacg ccatcaagta ggcttcaacc gcatcgacgt
     2581 ccggtccttc ggacgattcg aaattccgca ggtcgcccga tctaactccc cagcccccgc
     2641 acacacccac ctcaggatcg ttcaacgcct gatcgatcaa ctcaaagata tcacccgcga
     2701 tctcgacatt gttgcccagc ataatgatga tgcggccttg acttcgcatc agcccaacat
     2761 tccggcccgc cgcttcgccg atccaatgat cgatccacaa cgcctgcact tccggatgag
     2821 tagccgctat ttgcttaacg atctccgccg tgccatcgct tgatccgttg tcgattaaca
     2881 caatctcgat cgaatgctga ccccgattgg ctaacacgct cgatacggct cgttcgatct
     2941 catcggcatt gtcc
//
LOCUS       DASRTJ010000606         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_357873,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000606 DASRTJ010000000
VERSION     DASRTJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357873"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFF70_12395"
     CDS             <1..767
                     /locus_tag="VFF70_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZY45536.1"
                     /translation="TIVTEAQVQQKLVQTPEAQHLLRRRALRRATIVLGLVAALGLFL
                     LWLPLSSRRLLISSLVANQLLIGLLSIFGLIAISLLWAVGQRLDVWLFTALNLRGYHA
                     VWMDRLMWLATQIGNVGFAALLVVTAYVLGDHRFAIDLTLGSLTLWLLVTIIKALTDR
                     VRPFNLLRETRVIGWREPGLSFPSGHTTQTFFMMTLAISHFQLPFLVALGLYGIAVLV
                     GFTRIYLGMHYPRDVIAGAILGLIWGSLGVLVAPYL"
     gene            834..1661
                     /locus_tag="VFF70_12400"
     CDS             834..1661
                     /locus_tag="VFF70_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZY45537.1"
                     /translation="MADSVKAVKAIKKKTVETVKKPVEAVKKPVNAWRRTIDAAGAIV
                     EKPWRRYRATVFLTYLIVAVVAFIILAVLAKTVAYFTFDVTITHAIQGFNPGWFDALM
                     RALTWIGFSPQAWVITLGILLFLYASGLKWETVVAFVSVLGSSALGLGLKVLIDRPRP
                     SADLVTVLTQLKDFSFPSGHVLFFITFFGFLLFLAYTLLKPTRWRTVLLVILVGMIVL
                     IGPSRIYVGQHWASDVFASYLIGSVWLALSVLIYRWGKPRYFVNQPVAKETPVSQQP"
     gene            1658..>2642
                     /locus_tag="VFF70_12405"
     CDS             1658..>2642
                     /locus_tag="VFF70_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZY45538.1"
                     /translation="MIGPSAESPLELPTSRWTPRRVILGTLVVLSVVISFLLFYRFRL
                     IIVIVFSGVVVSMAIAPGVDWLHRHRVPRSLSVILIYLVLLLLLIGFIILLVPPIVEQ
                     LSTAVPKIENYYLELKAALVSSPLQVIRQIAAQMPAQINLTLAVSPAAGGSLDAVGQA
                     LTTAGSILSGLFTLTAILLIGFYWTLEGEGALRPLLRPLSNDKRESAQEMLQEIGTRV
                     GGYVRGQGILALTIGVADLIVFLAIGLPAALAVALLAGLFEMIPVLGPTLGAIPAVLV
                     AFAYDSSKVVWVIAAGALIQLLENNVLAPRVMHKTVGVNPIVTLLAIVAFGA"
BASE COUNT          509 a          674 c          738 g          721 t
ORIGIN      
        1 tgacaatagt aaccgaagcg caagtccagc aaaaactagt gcagacccct gaagcgcagc
       61 acctgttacg tcgacgcgca cttcgacgcg cgaccatcgt gctcggtctg gtcgccgcgc
      121 tcggcttgtt tcttttgtgg ctgcccttgt cgtcgcgccg cttgctcatc agcagtttgg
      181 tcgccaacca actgctgatc gggttgctca gtatctttgg gttgattgcg atttcactac
      241 tctgggcggt gggtcaacgc ctggacgtgt ggttatttac agccttgaat ttgcgcggct
      301 atcatgctgt ctggatggat cgcttgatgt ggctggcgac gcagatcggc aacgtaggtt
      361 tcgccgcgct actcgtggta accgcatatg tgttaggcga tcatcgtttt gcgattgatt
      421 taacgctagg ctcgctgacg ctgtggctat tggtcacgat catcaaagcc ttgaccgatc
      481 gggtgcgtcc gtttaacctg ctgcgcgaaa cgcgcgtgat tggctggcgt gagccgggct
      541 tatcatttcc cagcgggcat acgacacaaa cgttttttat gatgacactc gcaatcagtc
      601 attttcaatt accgttcttg gtcgcactag gattgtacgg catcgccgtt ttggtcgggt
      661 ttacacggat ttatctcggc atgcactatc cgcgtgatgt gatcgccggc gctattctgg
      721 gattgatctg gggcagctta ggagtgttgg tagcaccgta tctctagctg atcgtcttgc
      781 tgaaattcag gcacagatct ctgatgaaaa aatgttcgtt atgaggtaaa ttgatggctg
      841 acagtgtcaa agcagtcaag gcaatcaaga aaaaaacggt cgagaccgtc aagaaaccgg
      901 tagaggccgt taagaaacca gtcaatgcct ggcggcggac tattgacgcc gcgggcgcga
      961 ttgtcgaaaa gccatggcgg cgctatcggg ctacggtttt cctgacgtat ctgattgtcg
     1021 cggtagtggc cttcatcatt ctggcagtac tcgcgaaaac ggttgcctat tttacgttcg
     1081 atgtgacgat cacacatgcc atacaaggct tcaacccagg ttggtttgat gccctcatgc
     1141 gtgctttgac atggatcgga ttctcacccc aagcctgggt gatcacgcta ggtatcttgt
     1201 tgttcctcta cgcgagcggt ctgaaatggg agaccgtggt agcctttgtg agtgtgttgg
     1261 gcagctccgc gttgggactc ggcctcaaag tcttgatcga tcgcccgcga ccgagcgccg
     1321 atctggtgac cgtcctcacc cagttgaagg atttttcttt tccgagcggg catgtgctgt
     1381 tctttataac gttctttggc tttttgttgt ttctggctta cacgttgctc aaacctacgc
     1441 ggtggcgcac agtgttgctg gttatactcg tcggtatgat agtactgatc gggccgtcgc
     1501 gtatctacgt cgggcagcat tgggccagtg atgtgttcgc ctcgtacttg atcggcagcg
     1561 tttggctcgc tctctcagta ttgatttatc gctggggcaa accgcgctat ttcgtgaacc
     1621 agccagttgc aaaggaaaca cccgtcagcc agcaaccatg atcggaccat ccgctgaatc
     1681 tccgttagaa ctacccacgt ctcgctggac gccccggcgc gtgatcctgg gcacactagt
     1741 tgttctatcg gtcgtgatca gcttcctgct tttctatcgc tttcgcctaa tcatcgtcat
     1801 cgtcttttcc ggcgtcgtgg tgagcatggc catcgctcca ggcgtcgatt ggcttcatcg
     1861 acatcgagta cctcgatcgc taagtgtcat cttaatctat ttagtgctgt tgttgttact
     1921 gattggtttt atcattttgc tagtaccccc gatcgtcgag cagctatcca cggcagttcc
     1981 aaaaattgag aactattatc tggagttgaa ggcagcactg gtgagttcac cgttgcaagt
     2041 gattcgtcaa atcgcagcgc agatgccggc tcagatcaat ttgacactgg ccgtgtcacc
     2101 cgcggccggc ggctcgttag atgcggtcgg acaggcgctc acgactgcgg gcagcatact
     2161 gagtggactg tttacgctga ctgcgatttt gctgatcgga ttttattgga ctctcgaagg
     2221 cgaaggcgcg ctacgccctc tattacggcc attatcgaat gacaagcgtg agagtgccca
     2281 ggagatgctc caagagattg gaactcgcgt gggcggatat gtacgcggcc aaggaattct
     2341 cgcgctgaca atcggtgtcg cggacttaat cgtctttctg gcgatcggtt tgccggcagc
     2401 tctggcggta gcgctgttgg ccggactgtt tgaaatgatc ccagtgttag ggcccactct
     2461 gggcgcgatc cccgcagtgt tagttgcctt cgcctatgac tcgtcgaaag tcgtgtgggt
     2521 catcgcggcg ggggcgctta ttcaactgct tgaaaataac gtgctcgctc caagggtcat
     2581 gcataagacg gtcggcgtta atcccatcgt taccttatta gcgatcgtgg cttttggagc
     2641 tt
//
LOCUS       DASRTJ010000607         8440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_130144,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000607 DASRTJ010000000
VERSION     DASRTJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130144"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFF70_12410"
     CDS             <1..223
                     /locus_tag="VFF70_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45539.1"
                     /translation="VHALLSLAFLFNLITVAPFTPALGSNLVAASIDSPFVFVLKILS
                     LGAAAINILILIMLLIRLKQLPTAIESAV"
     gene            220..1383
                     /locus_tag="VFF70_12415"
     CDS             220..1383
                     /locus_tag="VFF70_12415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45540.1"
                     /translation="MKVVWIVPGFSSDERDWCIPALLDLARVMASQCELHIVSLRYPY
                     RRDRYSVYGATVHSIGGGHRGAWFTPSIWRAAQNVIDQLDFDVLHAWWLYEPGLLAAR
                     LKKRAPIVISLAGGELINLPSIGYGLARRFYLRWLMRWSLRRADVVTTGSNCLIEIAK
                     KFEAVKRIEFAPLGVDTTMFTPSRISGVEASAGAGVIEPAKASTPESRVILNVGSLEP
                     VKDQTTLLHAFKKVHDQLPNTRLIIAGEGNLHCPLSRLADTLNLTPFVEFRGEVPHHQ
                     LPDLYRSASVFVQSSRHESQGMALLEAAACGVPIVGTRAGTLSDLAPDAAFGTTIGNE
                     DELAAAIIGVLIQPDRAAEISQAARSIIKHEYALDRSVDRFRKIYRDLTGRHD"
     gene            1463..2770
                     /locus_tag="VFF70_12420"
     CDS             1463..2770
                     /locus_tag="VFF70_12420"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY45541.1"
                     /translation="MAALFFSPWILQWPILWPGLDETSVPILANLPKLRLADMLAVAS
                     IALYAFAGWSGLRQLGRRSVRWWLWSLTGLAILSVLSITWAMDTNLALVFAAHSVLWI
                     AFAVRVAADDLPPRSLALSLTAGMFVNSAICLLQFATQHSLGLTFFGELPLDPAAPGI
                     SVIGNNDIHLLRVYGLSGHPNVIGGFMAVALIWSLGLVARSSRRWLPMILGAWLLGWL
                     TLMLSFSRSAWLGIAFALGWMASLMFWRRVYRRYAGRVIAVVGIIVISILLLANMFNI
                     FLIERLTADQTSLLESVSIRERIDLIQAAVELIELRPLTGVGIGNFIAGSQRFIGPEV
                     GHDWVHNVPLLIASEMGLPGVTLWLIGLAAIGRSLVRTYRRSALDMWQIAAACSIAAM
                     LVIMQFDHYWWTSSQGVYMWAAITGWLMSQNSEASAPRSALGC"
     gene            complement(2767..3915)
                     /locus_tag="VFF70_12425"
     CDS             complement(2767..3915)
                     /locus_tag="VFF70_12425"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZY45542.1"
                     /translation="MRATGTTQFKTQPGLIGFDVTALAFGYHSGVGRYTRQLLAALID
                     RHDEHRYALLANKPVKGALPSGAIDRIQRRTVNRTWWLQLIVPRTIAQLQPELCHFTN
                     VVAPLNAPCPIVVTIHDAAILMNPQWQPLKSLLAVRAFLPSVARRADAIIAVSHSARR
                     DLITYLRLPPDKVRVVYEAAAPHYRPIENAAEIDRVRRRYQLHDPFALYVGTIEPRKN
                     LDRLVQAFGRIQKADRHQQLIMVGQLGWKYRAVLKLVEDLGLQDRVRQLGYVPDEDLP
                     AIYTLANFSVYPSLYEGFGLPIIESMACGTPVITSNRSSMAELGTGAALLIDPTQIDS
                     LADGWQQLLTDASLRSELRAAGLQRAAEFSWSRAAAETVEVYDSLRAD"
     gene            complement(3887..5620)
                     /locus_tag="VFF70_12430"
     CDS             complement(3887..5620)
                     /locus_tag="VFF70_12430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZY45543.1"
                     /translation="MNRVQSAKKSIALDQLGFISVCLLAFSLPFELARPIALIGPLII
                     SNVEIVLYAVLAFWLLSRIGTRRICWTRVHSAVLAWILTLILSAIFAPSNQTEALKFT
                     LRSIGGCLLFFSTVDLVKTNKQVSWIAAALAIGSCLSALSGIAEVQSAAVSQFLTAFK
                     TQPSILSGLVRAAGTFEYANIAAMYWEATLVATLGVSLWLTAQRGQMRWQYLGTAASI
                     GIAVAIVLSYSRAALIGSILELIILLAISHQTRSVSQRPIVIALIALIGTAAIMLFTN
                     PLFALRLKSETNLDWYLAEYQAINPPSTLEPNHSISVTVQITNRSIQEWPNNGERAIY
                     FGYHWLDDKQQIIIQHDSPHTPLPYAVAQDSVVTVVLRITAPVEPSTYFLQWDLLQEN
                     VTWFSSVTGQASGLLVSVTANGVAQQPPTVISQPAAGYPLGKALPPRSDLWRVAFQMW
                     REHPILGIGPDNFRHLYGSYLGLSIFNDTVTTNSLYIEILTNVGMLGLASFLAILCAA
                     FTFIRRTWHTLTEPANRWLAASLWLAFAAFLIHGLVDTFLAFTPTYGLFWLVCGLLVS
                     VLNESDRHDTV"
     gene            complement(5617..6888)
                     /locus_tag="VFF70_12435"
     CDS             complement(5617..6888)
                     /locus_tag="VFF70_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZY45544.1"
                     /translation="MKTYSSGPPAPDLLSRRLRINAGSKLAAEIVGRALQFLLAYLAQ
                     RTLGPAGYGDFTYALSVSVVLSPLTDLGLQLIITREIARNAAQADRIAGAGLMLKLAL
                     TFAAGLILISIALSRPIDLQAATFTLGLALMFNSVVEYIGYIFRGLQRVEYEAAVTLL
                     LRILTVILGIGALALNFGLIGLTLAYLISSGIAAAISYLLLRRKFFKPRFKIDLRYSW
                     LLLRQALPLGGAIVLSILYTRVAVFLLQPLKGSAAVGLYGVAQKLTEALAILPAAILA
                     AVFPAFTQAMTNNIGQARWLQRRTLLLLFGAGVVIAICGWWGGSWLIHFLYNNQFAGS
                     TAALQILAIAVLFTFINYALTHFLVALNLQRWILIFNMAVLALDVAACLILIPPYGPA
                     GAALASLISECLLGLLCGWALWRHPLDRMAK"
     gene            complement(6975..7046)
                     /locus_tag="VFF70_12440"
     tRNA            complement(6975..7046)
                     /locus_tag="VFF70_12440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7011..7013),aa:Arg,seq:ccg)
     gene            7220..8023
                     /locus_tag="VFF70_12445"
     CDS             7220..8023
                     /locus_tag="VFF70_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HZY45545.1"
                     /translation="MYPRSTWRLLNTHYLDGATNMAIDEAISRAVVAGLVPPTLRFFG
                     WQPACLSLGQAQPGSDVDREACRINGIDVVRRPTGGHAILHTDELTYSVIALDADPRV
                     AGTIVESYQRLSEGLLNGLKLMGVPTHQADRVDGHDRDQGPVCFEVPSNYEIVFGDKK
                     LVGSAQMRKAGVVLQHGTIPLHGDIARITHYLSSQPDPDRVRSRATTVEAAIGCVIDF
                     EDAARWMAEGLAQTLEIDLLPGELIDQERDWTDELRQNKYATHEWTFRM"
     gene            8122..8406
                     /locus_tag="VFF70_12450"
     CDS             8122..8406
                     /locus_tag="VFF70_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZY45546.1"
                     /translation="MIVGVCTIELHLPANGSLKGKRAALKPLLARLHREFNVSAAEID
                     QQDSWQESTIALACISNDPTQAEQVLNHAVSWIELNRPDVQLVDWRIEIL"
BASE COUNT         1925 a         2196 c         2372 g         1947 t
ORIGIN      
        1 ggtgcatgct ctcttgtcgc tggcattcct ctttaatttg atcaccgtcg ccccgttcac
       61 tccagccttg ggcagcaatt tggttgcggc gtcgattgat tcgccgttcg tcttcgtgct
      121 gaaaatttta tcgctgggtg cggccgcaat caatatactg atcttgatca tgctgctgat
      181 tcgattgaaa caacttccga ctgcaataga gagcgcagta tgaaagttgt ctggattgtg
      241 cccggcttca gcagtgatga acgcgattgg tgcattcccg cgctgctcga tctggcgcgg
      301 gtcatggcat cgcaatgtga attgcacatc gtttcgctgc gctatcccta ccgccgcgat
      361 cgatattcag tatatggtgc aaccgtgcat tcgatcggcg gcggacatcg tggggcgtgg
      421 tttacgccgt cgatctggcg ggcagcgcag aacgtaattg atcagttgga tttcgatgtc
      481 ctgcacgcgt ggtggttata tgaaccgggg ctacttgcag cgcgattaaa aaaacgtgcg
      541 ccgatcgtga tcagcttagc gggcggcgag ttgatcaact tgccctcgat cggttatggc
      601 ttggctcgac ggttttatct gcgctggctg atgcgttggt cgcttcgccg cgcggatgtg
      661 gtcacgaccg gatcgaattg cctgattgag attgcgaaaa aatttgaagc agtgaagcgc
      721 attgagtttg cgccgttggg tgtggataca acaatgttta ctccatcgcg catttcgggc
      781 gtcgaggctt cagccggtgc aggcgttata gaaccggcta aagcctcgac gccggaatca
      841 agggttatcc tcaacgtcgg ttcacttgaa ccagtcaaag atcaaaccac cttgcttcat
      901 gcgtttaaga aagttcatga tcaactgccc aacacccgct tgatcatcgc tggcgaagga
      961 aatctgcact gcccattaag ccgtttggct gacacattga atctgactcc tttcgtagaa
     1021 tttcgcggcg aagtgccgca tcatcaattg ccggacttat atcgatcggc ttcagtcttc
     1081 gtgcaatctt cgaggcatga atcgcaaggg atggcgttgt tggaagcagc agcatgtggc
     1141 gtgccgatcg tcggtacgcg cgctggaact ttgtctgatc tcgcgccgga tgccgcgttc
     1201 ggaaccacaa ttggtaatga agacgaattg gcggctgcaa taattggggt gttgatccag
     1261 cccgatcggg ctgccgagat tagtcaggca gctagatcga tcattaagca cgaatatgcg
     1321 cttgatcgat cggttgatcg gtttagaaaa atttatcgag atcttacggg cagacatgat
     1381 tgaatccatt gcaacgcctg atcattctga aattgttatt actcggcacc cgcgactaat
     1441 cgcgcttagc cgcgggtgcg tcttggccgc gctattcttc tcgccgtgga ttttgcagtg
     1501 gccaattctt tggccgggct tggatgaaac gtctgtgccc atcttagcta atctaccgaa
     1561 gctgcggctg gccgatatgc tggctgtggc atccatcgcg ttgtatgcat ttgccggctg
     1621 gtcagggttg cgccagttag ggcgcaggtc tgtgcgctgg tggttatggt ctctgacggg
     1681 tttggcgatc ctgagcgtac tgtcgatcac ttgggcgatg gataccaact tggcacttgt
     1741 gtttgcggcg cattcagtat tgtggatagc gtttgcggta cgtgtggcag ctgatgatct
     1801 tccacctagg tcactcgcgt tgagtttgac agccgggatg tttgtcaatt cggcaatctg
     1861 cttgcttcaa tttgcaacac aacacagttt gggcctaacg tttttcggcg aattgccgct
     1921 cgacccggcc gcgccgggta tcagtgtgat cggcaataac gacatccact tattgcgggt
     1981 ttatggactc agcggtcatc ccaatgtcat tggtggtttt atggccgtgg ctttaatatg
     2041 gagcctgggc ttggttgcgc gatcgtcgcg ccgctggttg cccatgattt tgggcgcgtg
     2101 gctgctgggc tggctgaccc tgatgctctc tttttcgcgc tcggcttggc taggcattgc
     2161 ctttgcttta ggctggatgg ccagtttaat gttctggcgg cgcgtgtatc gccgctatgc
     2221 cggtcgagtg atcgctgtag tcggaatcat agtgatctca atcttgctgc tggcgaatat
     2281 gttcaatata tttttaatcg aacgcctgac cgcagatcaa acgtcactgc tggagtcggt
     2341 ttcgatcagg gagcgaatcg atctcataca ggccgccgtt gagttgatcg aattgcggcc
     2401 gttgaccggt gtcggcattg gcaattttat tgcggggagt caaaggttta ttggccccga
     2461 ggtcggacac gactgggtgc ataatgttcc tttgttgatt gccagtgaga tggggctgcc
     2521 gggtgtgacg ctatggttga tcgggttggc tgcaattggc agaagtctgg tccgcactta
     2581 tcgacggagt gcgttggaca tgtggcaaat cgcggcagcc tgttcgatcg cggcgatgtt
     2641 ggtgatcatg cagttcgatc attattggtg gacttcttcg cagggtgttt atatgtgggc
     2701 tgcgatcacc ggctggttga tgagccagaa ttccgaagcg tcggctccgc gaagcgccct
     2761 tggttgttag tcggctctta atgaatcata aacctcaaca gtctcggccg ccgcgcgcga
     2821 ccacgaaaat tctgccgctc gctgcagacc cgccgcccgt agttccgagc gcaacgatgc
     2881 atcagttagc agctgctgcc aaccgtctgc cagcgaatcg atttgtgtgg gatcgatcag
     2941 cagcgccgcg cccgtgccca actctgccat cgacgatcga ttggaagtga tcaccggtgt
     3001 cccgcaagcc atgctttcaa tgatcggcaa gccgaagcct tcatacaacg acggatacac
     3061 gctgaagttt gccagcgtgt aaatagcggg caggtcctca tcgggcacgt aacccaattg
     3121 gcgcacgcga tcttgcaagc ccaaatcttc aaccagcttc agcaccgccc gatatttcca
     3181 gcccaattga ccgaccatga tcagttgctg atgacgatcg gccttttgaa tgcgcccaaa
     3241 ggcctgcact aatcgatcga gatttttgcg cggctcgatt gtgccgacgt ataacgcaaa
     3301 tggatcatgc agctgatatc ggcggcgaac ccgatcgatc tcggctgcgt tttcgatcgg
     3361 acggtaatgc ggggctgcgg cttcatacac cacgcgcact ttatcgggcg gcagccgcag
     3421 ataagttatc agatcgcggc gcgcactgtg cgacacggcg atgatcgcat cggcgcggcg
     3481 agcaaccgac ggcagaaagg cgcgcaccgc cagcagactc ttcaaaggct gccactgtgg
     3541 gttcatcaag atcgccgcat cgtgaatggt gacgacgatc gggcaagggg cgttcagcgg
     3601 tgcgacgacg ttcgtgaagt gacacaactc cggctgcaat tgtgcgatcg tgcgcggtac
     3661 aattaattgc agccaccacg ttcgattaac ggtgcggcgc tggatgcgat cgatcgcgcc
     3721 gctcggcaga gcgcctttca ccggcttgtt cgccagcagc gcgtagcgat gttcgtcgtg
     3781 tcgatcgatc agcgccgcga gcagctgccg cgtatagcgc cccacgcccg aatgataacc
     3841 gaacgccagc gccgttacat cgaagccaat caatcccggt tgagtcttaa actgtgtcgt
     3901 gccggtcgct ctcattcaac acactcacta ataagccgca cactagccaa aacaaaccat
     3961 acgtcggcgt gaacgccaag aacgtatcga ccagaccatg gattaaaaac gcggcgaagg
     4021 ccagccacag acttgcggcc agccagcgat tggccggttc tgtcagagtg tgccacgtgc
     4081 ggcgtatgaa ggtgaacgca gcgcagagga tagccaaaaa agaggctaga ccaagcatgc
     4141 cgacgttcgt cagaatttca atatacaggc tgttcgtcgt cactgtgtcg ttgaagattg
     4201 agagccccaa ataggaccca tacagatgcc ggaaattatc aggtccgatc cccaagatcg
     4261 gatgctcgcg ccacatttga aacgccacac gccataaatc cgatcgcggc ggcagcgcct
     4321 tacccagtgg gtatccggct gccggttggc tgatgacggt gggcggttgt tgagccactc
     4381 cattcgccgt cacactgacc agcaagccac tggcttgccc cgtcactgag ctgaaccacg
     4441 tcacattttc ttggagcaaa tcccattgca gaaaatatgt gctgggttca accggtgcgg
     4501 taatacgcag gacaacagtc accacggagt cctgcgccac cgcgtagggt aaaggagtat
     4561 gcggggagtc atgctggata attatttgct gcttgtcatc cagccaatga tatccaaagt
     4621 agatcgctcg ctcaccatta ttcggccatt cttgaatgct tcgattagta atttgcaccg
     4681 ttacgctgat gctgtggttt ggctccagag ttgacggtgg attgatagcc tggtactcgg
     4741 ccagatacca atcgaggttg gtttcagatt tcaaccggag cgcaaacagt ggattggtga
     4801 acagcatgat cgctgcagtc ccgatcaatg cgatcagagc gatcacaatc gggcgctgtg
     4861 aaaccgatcg ggtttgatgg ctgatcgcca acagaatgat caattccaag atcgaaccga
     4921 tcaatgctgc ccggctgtag cttaacacga tcgcaactgc aattccgatg gaggcggcag
     4981 tgcctagata ttgccagcgc atttggcccc gttgtgcggt taaccaaaga ctgacgccga
     5041 gtgtagccac gagcgtggcc tcccagtaca tggctgcgat attggcgtac tcaaatgtgc
     5101 cagctgcgcg caccaggccg ctgaggattg atggctgcgt tttaaatgct gtcaaaaact
     5161 gtgagacagc cgcgctttga acttcagcaa tgccgctcag tgcggagaga catgacccaa
     5221 tggcgagcgc agctgctatc caactgactt gtttgttggt cttgaccaga tcgaccgtcg
     5281 agaagaatag tagacagccg ccaatacttc gtaatgtaaa tttgagcgct tcggtttgat
     5341 tgctaggtgc gaatatcgcc gacaggatca gggtcagtat ccaagctaat acagctgaat
     5401 gaacacgtgt ccagcagatt cgccgggttc caattcggct gagcagccag aatgccaaaa
     5461 cagcatacag cacaatctcg acgttgctga tgataagtgg accgatcaat gcgatcggcc
     5521 gggcaagctc gaagggcaaa ctgaaggcca gcaggcacac actgatgaaa cccagctgat
     5581 ccagcgcaat tgattttttg gcggattgta ctcggttcat ttagccatcc gatccagcgg
     5641 atgacgccat agcgcccacc cacataacaa gccgagtagg cattcactga ttaaactggc
     5701 aagtgctgcg cctgctggtc catatggcgg aattaagatc aagcaggcag caacatcgag
     5761 agcgagaaca gccatattaa agatcaaaat ccagcgctgc aggttgagcg cgacgagaaa
     5821 gtgggtcaaa gcataattga taaaagtgaa caagaccgcg atcgccaata tctgcaaagc
     5881 ggcggttgaa ccggcaaatt gattgttata taaaaaatgg atcaaccatg atccgcccca
     5941 ccagccgcag atcgcaatga ctacgcccgc accgaacaat agtagcagcg ttcgacgctg
     6001 aagccatcgg gcttgaccga tattgttggt catggcttgc gtgaatgcgg gaaacactgc
     6061 tgctaagatg gcagcgggca gaatggctaa cgcttcggtt agtttctgcg ccacgccata
     6121 taagccaacg gccgctgatc cttttaacgg ctgcaacaaa aaaacggcga cgcgcgtata
     6181 caagatcgac aagacgatcg cgccacctaa tggcaatgcc tgtcgcaata gtagccagct
     6241 ataacgcaga tcgatcttga aacgcggctt gaaaaatttt cggcgcagca agaggtaact
     6301 gatcgctgcg gcaatgccac tgctgatgag ataggccaac gttaaaccga tcagaccgaa
     6361 gttcaaggct aacgcgccga tccctaggat cacggtgaga atgcgcagca gcaaagtcac
     6421 agcggcttcg tactccacgc gctgcaagcc tcggaaaatg taaccgatgt attcaaccac
     6481 agagttgaac atgagcgcca gccccagcgt gaaagtggcg gcttgcagat cgattgggcg
     6541 ggatagggcg atcgagatca agatcaaacc ggcggcgaag gtcagggcca atttcaacat
     6601 caagcccgcc ccagcaatcc gatcggcctg agctgcgtta cgggcgatct cgcgggtgat
     6661 gatcaactgc aagccgagat cggtcaaagg cgataagacc acgctcaccg aaagcgcgta
     6721 agtgaagtct ccatagccgg ccggaccgag cgtccgctgc gcgagatagg ctaacaggaa
     6781 ttgcaatgcc cgcccgacga tctcggcagc gagtttgctg ccagcgttaa tacgcaagcg
     6841 gcggctgaga agatctggcg caggcggccc ggaagaataa gtcttcatcg caataatggt
     6901 gaagtcaacg gtgtcgttcc tcaaaaggta aaaaaacgcg agtgagaaaa tcactcgcgt
     6961 tgtctaagaa ggtgtaccct cggtgagaat cgaactcacg ccatcggctt cggaggccgg
     7021 tgctctatcc gctgagctac gagggtattg atcgttagtg taccacatgc ccgatcgacc
     7081 cgcaaactcg gcgtgttata atttctctcg tagtgcgcaa agtataatgc gtgttgcgtg
     7141 gggcgcattg atcaaatcaa tttagcgctt accacccgtc acacatcatt catcgttaat
     7201 tgctcattgc tgattaccta tgtatcctcg ttccacttgg cgactgctta atacacatta
     7261 cctcgacggc gcaaccaaca tggcgatcga tgaagccatc agccgggcgg tggtggctgg
     7321 ccttgtcccg ccgacactgc gctttttcgg ttggcaaccg gcgtgtttat cgttgggaca
     7381 ggcccaaccg ggcagcgatg tcgatcgcga ggcctgccgc ataaatggta tcgatgtggt
     7441 gcgccgcccg accggcggcc acgcgatctt gcacactgac gagttgacgt atagtgtgat
     7501 cgccctggat gctgatccgc gtgtggcggg aacgattgtt gaatcatacc agcgactgag
     7561 tgaaggctta ttgaacggtt tgaagttaat gggcgtgcca acgcaccaag ccgatcgcgt
     7621 cgacggacac gatcgcgatc aaggcccggt gtgttttgaa gtgccctcca attacgagat
     7681 tgtgtttggc gacaagaaat tggtcggcag cgcgcagatg cgtaaagctg gcgtcgtctt
     7741 gcagcatggc acgatcccgc tgcatggcga tattgcgcgg atcacacact atctttcgtc
     7801 tcagccggac cccgatcgag tgcgcagccg cgcgacgacg gttgaagccg cgatcgggtg
     7861 cgtgatcgat tttgaggatg ccgctcgctg gatggctgaa ggcttggccc aaacgctcga
     7921 gatcgatctg ctgcccggtg agttgatcga tcaggagcgg gattggacag acgaacttcg
     7981 tcaaaataag tacgccactc acgaatggac gtttagaatg taatcaataa cgagtcatga
     8041 gtaaattcgt attacacgtc acttgttact cgttatcttc ctcgcttcac ttttcacttc
     8101 gggcatgtta gaattccaag catgatcgtc ggcgtctgca ccattgaact gcatttgccc
     8161 gcaaatggat cgctaaaggg caaacgcgcc gcgctcaaac cgctattggc tcgcttgcac
     8221 agagaattca atgtgtcggc tgccgagatc gatcagcagg atagttggca agagtcaacc
     8281 atcgcactgg cctgtatatc taatgacccg acccaggccg agcaagtttt gaatcatgcg
     8341 gtaagttgga tcgaactcaa tcggccggat gtgcagctgg tcgattggcg gatcgaaata
     8401 ctctagcgga ctcaaccgat ggcatataca gaactgcagc
//
LOCUS       DASRTJ010000608         6847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_205807,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000608 DASRTJ010000000
VERSION     DASRTJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205807"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..894
                     /gene="lepB"
                     /locus_tag="VFF70_12455"
     CDS             304..894
                     /gene="lepB"
                     /locus_tag="VFF70_12455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZY45547.1"
                     /translation="MSEEIQLEKVEVATPANETPKTRSNLLREIIETALLTAIIFLLV
                     NTLTGRFRIEGSSMEPNLHDGEYVIIDRVSYVLHQPERGDIIVFTPPTGDRDYIKRVM
                     ALPGDSVEVSNGQVLVNGTALNEPYLNTTTNYALGSQTIEPGHYFVLGDNRNNSSDSH
                     AFGTIVNSAIVGRAWFVYWPPSNWGAVPHYTYAAAK"
     gene            934..1536
                     /gene="lepB"
                     /locus_tag="VFF70_12460"
     CDS             934..1536
                     /gene="lepB"
                     /locus_tag="VFF70_12460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZY45548.1"
                     /translation="MSQELLNLAATSAETVPLSVEPLPKPSTLLRSIIETFVLMAIIF
                     LMVNTAIGRFQIQGPSMQPNLHEGERVIVDKVTYWLRPPQRGDIIVFDGHGAVDLIKR
                     IIALPDETIEIHGGQVSINGIVLDEPYLEHPTSSDVSAQQVAADHYYVMGDNRDNSQD
                     SRAFGAITSKEIVGRAWIIYWPPTNWLIVPHYNYAAAQTH"
     gene            1584..2270
                     /gene="rpiB"
                     /locus_tag="VFF70_12465"
     CDS             1584..2270
                     /gene="rpiB"
                     /locus_tag="VFF70_12465"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZY45549.1"
                     /translation="MVRAVLLRTLGLTEQSAAPRSSVTISASMIDQLAPGKSITLDSG
                     DRITPLARDAARERGVQLIEHPIDLPVRSATHKSIALGSDHGGFELKHTLHTYLIELG
                     YAPIDCGTFSTDSVDYPDFALAVAELVARGRAGCGIIIDGAGIGSCIAANKVPSIRAA
                     MCYDQATAVNSREHNDANVLTLGAGLIGSTLAKQIVKTFLDTPFGGGRHAKRVDKIVA
                     IEKRYSKSNR"
     gene            2528..3205
                     /gene="deoC"
                     /locus_tag="VFF70_12470"
     CDS             2528..3205
                     /gene="deoC"
                     /locus_tag="VFF70_12470"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZY45550.1"
                     /translation="MLPHDEGLASYIDHTLLKPDATDDQIAQLCYEARKYNFAAVCVN
                     PSKVKLCAQLLKGASVKIATVVGFPLGATPPEVKAYESQQALEDGANEIDMVINIGAL
                     KSKDYSLVGRDIATVVRTAHIGGAITKVIIEAALLTDEEKAIACKLAKEAGAEYVKTS
                     TGFSSGGATVHDVALMRWAVGPEMGVKAAGGIKTYADALKMIEAGATRVGASASVKIM
                     QEAEKNN"
     gene            3248..3559
                     /locus_tag="VFF70_12475"
     CDS             3248..3559
                     /locus_tag="VFF70_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45551.1"
                     /translation="MLVFGQSSFIKKNMLTCPDCRTGQLQSKFICYANWHAGRFVIAP
                     YTPAWWCAVCGHIELQAAAVNRLLSLLGPVSRPSAAHTRLGVQINFDLFQNLSSTRKY
                     Q"
     gene            3586..4221
                     /locus_tag="VFF70_12480"
     CDS             3586..4221
                     /locus_tag="VFF70_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019494149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45552.1"
                     /translation="MVAELRSYVFLDSLQPQHAAFLGTVAQGFLPLPHDASLWVEISP
                     GIEINRITDIAVKATGVRPGMQIIERLYGLLEVHSESQAEVRAAGAAILQALDLKEED
                     RWKPRILSSQIIRAVDPLHAQLINRMRHGHMLLGGHSLYVLEVEPAAYAAIAANEAEK
                     RANINILEVRAFGSFGRVYLGGEEQDIMAGYTAAVASIEGISGKETMGRKE"
     gene            4350..4658
                     /locus_tag="VFF70_12485"
     CDS             4350..4658
                     /locus_tag="VFF70_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HZY45553.1"
                     /translation="MAEALGMIECKSFAAVVEASDAMVKAAKVELVSYEKTGGGFVTA
                     VVRGDVAAVKAAVEAGVRGAEKVGEVVAVHVIPRPHVNVDLVLPLGRADQAAKEAKGQ
                     "
     gene            4733..5023
                     /locus_tag="VFF70_12490"
     CDS             4733..5023
                     /locus_tag="VFF70_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HZY45554.1"
                     /translation="MLLGKVIGTLVATRKDEKLEGLKFLVVRQITMDGKESANYVVAV
                     DAVGAGVGEVVMYASGSSARQTKITDQRPCDAVIMAIVDTWEVSGEVKYTKD"
     gene            5043..>6847
                     /locus_tag="VFF70_12495"
     CDS             5043..>6847
                     /locus_tag="VFF70_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45555.1"
                     /translation="MDQPGTRSPVDHREGLLTVPQALTRGSFTREQIEAVRAAAMAIT
                     ARLTVEQVLVIVAEQAAKSVNAAASLVYLVDTLTGELRLIAGFNVPELSIGFMLKPDE
                     NLAGPAALSGQPQIASDPTMLAEVRATFERIAPKSVAPDICSAAAVPLIYSRQVLGVL
                     EVIAIDGLIIDADQIALLQLLASHAATAVTHAQLHDRNQQVMSLLEMINDRSAAVSSV
                     GQAVINAGHDQTRMSLEVLTRTIASLHLAGGKVFLEDPITHQLAGIASENLPREDIVA
                     GLELAAAHCLGIRQTILLQNMSAQAWTREVTQWLSDHHFGRLVCIPLIADNEPVGVLQ
                     VAAKTDQAIETGMLDTLHIIAGQLALGVANARLFNRVRAEQQQLAAILSSSSDVLIGL
                     NANGDIQLANPAAERTFDFVAQAVIGQPLRRATQNATLNNAVDSAIKSKQRQPSGMEI
                     PLTDDQTLFCNLSPIIDPLGQLAGWVAVMQDITHFKEAERSKTDMILMASHDLRNPVN
                     LTLGALDLLGRSASNFNAIQREAYELSLLGLHRIEALISNLLDLERIEQRIGLNLTKC
                     DLTETARSVVNEMRLPAQQREQKLWLTLASTGNIP"
BASE COUNT         1650 a         1801 c         1892 g         1504 t
ORIGIN      
        1 caatacaaca tctctattca tgaattgatc tccgctcaat ttttggagat tacaacttag
       61 aggctagaag tcgatggcga catcttacac gagaaatctc cgatcgttcg gctgcgttca
      121 gggcatatcg ccaaattcta acttcctcat tatggcacag cctgtaaagg tgtcaagtca
      181 cattggtttt gtccctatgt ttgccacgcc caatgagtta gagtataatc gccaaagata
      241 gtttaggcga tcaaattaag cgcttagatc gctgtctgag atctgatggt cgaggtgaat
      301 gtagtgtccg aggagataca actggaaaaa gtggaagtgg caacgcctgc caatgaaacg
      361 cccaagaccc gatcgaattt attgcgcgag attatcgaaa ccgcgctgct gactgcaatt
      421 atttttttgt tggtcaacac cttaaccggc cgctttcgaa ttgagggttc cagcatggaa
      481 ccgaatctgc atgacggcga atacgtaatt atcgatcgag tgtcgtatgt actgcatcag
      541 ccggaacgcg gcgacatcat tgtcttcacc ccgccgactg gcgaccggga ttacatcaag
      601 cgtgtcatgg ccttacccgg cgatagcgta gaagtgagta acggccaagt gctggtcaat
      661 ggcactgcgc tgaacgaacc gtatctgaat acgaccacca actatgcgct ggggtcgcag
      721 acgatagagc cgggtcacta ttttgtctta ggcgataatc gcaataattc cagcgattca
      781 cacgcgttcg gtaccatcgt caacagcgcc atcgtcggcc gcgcatggtt tgtgtattgg
      841 ccacccagca attggggcgc tgtgccgcac tacacttatg cggcggcaaa gtaactgcgc
      901 tggtatccat tatattggaa taaaggttgg tttgtgtcac aagaattact taacctggcg
      961 gccacctccg ccgaaacagt tccactgtcg gtcgaaccat tacctaaacc gtcgacttta
     1021 ttgcgcagca ttattgagac ctttgtctta atggccatta tttttctgat ggtcaacact
     1081 gccatcggcc gttttcaaat tcaaggacca agcatgcagc cgaatttgca tgagggcgaa
     1141 cgggttatcg tcgacaaagt tacgtactgg ctgcgtcccc cgcagcgcgg cgacatcatt
     1201 gtgttcgatg gtcatggcgc ggttgatctg atcaagcgca tcatcgccct gcccgacgaa
     1261 acgatcgaga ttcatggcgg tcaagtgtct atcaatggta ttgtgcttga cgagccttat
     1321 cttgagcatc caaccagcag cgatgtctcg gcgcagcaag tggccgccga tcattactat
     1381 gtcatgggcg acaatcgaga caattcgcaa gactcacgcg ccttcggggc tatcacttcg
     1441 aaggaaatcg ttggccgggc atggattatc tactggccac caacgaactg gttgattgtg
     1501 ccgcattaca attacgcggc ggctcaaaca cattaagcca gagcgaggca tgcgtgtcga
     1561 tttctgaggc gcaggttcga gaaatggtcc gagccgttct gctccgcacg ttgggcctga
     1621 cggaacaatc ggccgcgccg cgatcctctg tgacaatctc agccagcatg atcgatcaac
     1681 tcgcgccagg caaatcgatc acgctggact ccggcgatcg aatcacgcca ctggcgcgcg
     1741 acgccgcacg tgaacgcggc gtgcagttga tcgaacaccc aatcgatctt ccagtccgat
     1801 cggccacgca taaatcgatc gcgcttggct ccgatcacgg cggctttgag ctaaaacaca
     1861 ctttgcatac ctacttaatc gaacttgggt atgcgccgat cgattgcggc acgttctcaa
     1921 ccgactccgt cgactatcct gattttgcgt tggccgtcgc ggagctggtc gcccgtggac
     1981 gcgccgggtg cgggatcatc atcgatggcg cgggcattgg ctcgtgcatt gctgcgaaca
     2041 aagtacccag cattcgcgcc gcaatgtgct atgatcaagc tacagcggtc aacagccgcg
     2101 agcacaatga cgccaatgtt ttgacgctcg gcgctggcct gatcggctcg acgctggcga
     2161 agcaaattgt gaaaacattt ttggacacgc catttggcgg cggccggcat gccaagcgcg
     2221 tcgataagat tgtggcgatt gaaaaacgat attcaaaatc aaaccgctga ggcgctgaga
     2281 cgcgaagaaa caatattgaa ttctcagtga ctgcgcggtt caaaccatta tgcaaatcga
     2341 taacgcgaca atcgagaaac tcatcgaaca gatcacgcgg caagtgctgg ttgagttgaa
     2401 tcaaccggct cacaacgcca gcggcaaagt ttgcaactgc ggcggcagcg cctgtgtgcg
     2461 agattgtgcc gatgatgtgc agcgcgtgat gaaagcgggc gcagcccgga tcacgtcgag
     2521 tgtcggcatg ctgccacacg atgaaggttt ggcgagttat atcgatcaca cgctattgaa
     2581 gcccgatgca accgacgatc agatcgcgca gttgtgttac gaagcgcgga agtacaactt
     2641 cgcggcagtg tgcgtgaatc ccagtaaagt gaagttgtgt gcccagctgc tcaaaggagc
     2701 ttccgttaag atcgccacgg tggtcggctt tccgctcggc gcgacgccgc ccgaagtgaa
     2761 agcgtatgaa tcccagcaag cgcttgagga tggcgcgaac gagatcgaca tggtgatcaa
     2821 catcggcgca ctgaagagca aagattactc cctggttggc cgcgatatcg ctaccgtcgt
     2881 tcgcaccgcc cacatcggtg gtgcaatcac caaagtgatc atcgaagccg ctttgcttac
     2941 tgatgaagaa aaagctatcg cgtgtaaact cgctaaagaa gccggcgcag agtacgtgaa
     3001 gacctccacc ggtttttcgt ctggcggtgc gaccgtgcac gatgtggcgt tgatgcgctg
     3061 ggccgtcggg ccagagatgg gcgtgaaagc ggcaggcggg atcaaaacgt atgcggatgc
     3121 actgaaaatg atcgaggcgg gcgcgacgcg cgtcggcgca agcgccagcg tgaaaatcat
     3181 gcaagaagca gaaaagaaca attagcaatg atcaatgtgc aattaacaat gatcactggc
     3241 aagtacagtg ttagtctttg gtcaatcgtc gttcatcaag aaaaacatgc tgacttgtcc
     3301 tgactgccga accggccaac ttcaatcaaa attcatttgc tatgccaatt ggcatgcagg
     3361 ccgtttcgtc atcgcgccgt atacgcctgc gtggtggtgt gcagtgtgtg gtcatataga
     3421 actgcaagcc gcagcggtga atcgattgct gtcgctactg ggtccggtct cacgcccaag
     3481 cgccgctcat acccgattgg gtgtgcagat caattttgat ctttttcaaa atctatcatc
     3541 gacgcgcaag tatcagtgat cgatctacaa cactggagat gataaatcgt ggccgaactt
     3601 cgatcttacg ttttcctgga tagtttgcag ccgcagcatg ccgcttttct aggcacggtg
     3661 gcgcaaggtt ttctgcccct gccccacgat gcatcattgt gggtggaaat ctccccgggc
     3721 atcgagatca atcggattac ggatattgcc gtgaaagcca cgggcgtgcg gcccggcatg
     3781 cagatcatcg aacgcttata tggcttgctg gaggtccatt cggaatcaca agccgaagtg
     3841 cgcgctgcgg gcgcggcgat attgcaggcg ctcgatctaa aagaagaaga tcgctggaaa
     3901 ccgcgcatct tgtccagcca gatcatccgc gcagtcgatc cacttcacgc gcagttgatc
     3961 aatcgtatgc ggcacggcca catgctgctg ggcggccact cgttgtatgt gctggaagtg
     4021 gaacccgcgg catacgccgc aatcgccgcg aacgaagcgg agaagcgcgc caacatcaac
     4081 attctggaag ttcgcgcgtt tggctcgttt ggccgcgttt atctgggtgg cgaagagcag
     4141 gacatcatgg ccggttatac ggcggctgtg gcctcgatcg aaggcatcag cggcaaggaa
     4201 acgatgggac ggaaagagtg atcagtaatt tgtaattaga atcgaattgc aaaaacactt
     4261 tactactcat ttggcactaa tcaatatctt aagtaattta atttgaattc cggctattca
     4321 ataagatctg aatcccatag gaggacgtaa tggcagaagc attaggcatg atcgaatgca
     4381 agagttttgc agccgtcgtc gaagccagcg acgcgatggt gaaagccgcc aaagttgagc
     4441 tggtcagtta cgagaagacc ggcggcggct tcgttaccgc cgttgtgcgt ggcgacgtgg
     4501 cggcggtaaa ggcggccgtt gaagccggcg tgcgcggagc cgagaaggtt ggcgaagtag
     4561 tcgcggttca cgtcattcct cgcccgcatg tcaacgtcga tctggtgctg cccttaggcc
     4621 gcgccgatca agccgccaaa gaagctaaag gacaatgaca aattcagaat gacaagtgac
     4681 aagacctctc attaatcttt tgtcatttga tcattagtca ttggaggttc acatgctgct
     4741 cggcaaagtg atcggcacat tggtcgccac gcgcaaggac gagaagctgg aaggcttgaa
     4801 gtttctcgtc gtgcgccaga tcacgatgga cggcaaagaa tcagctaact acgtcgtcgc
     4861 cgtcgacgcc gtgggcgctg gggttggcga agtcgtcatg tacgccagcg gcagttccgc
     4921 gcggcagact aaaatcaccg atcaacgccc gtgcgacgcc gtcattatgg cgatcgtcga
     4981 cacgtgggaa gttagcggcg aagtgaaata cactaaagac tgagcgagaa ccgatgagct
     5041 caatggatca gcctggaacc cgatcaccag tcgaccaccg cgagggcctc ttgaccgtgc
     5101 cgcaagcgct gacacgcggt agcttcacgc gcgaacaaat cgaagcggtg cgcgcagcgg
     5161 ccatggcgat tacagccaga ttgaccgtcg aacaggtttt ggtcatcgtc gccgagcaag
     5221 ctgcgaagtc ggtcaatgca gcggccagtt tagtgtatct ggtcgatacc ctcactggcg
     5281 agctgcgttt gatcgcgggc tttaatgtgc cggaactatc aatcggattt atgcttaagc
     5341 ccgatgagaa cctggccggg cccgcggcct tatcgggcca accgcagatt gcttcagacc
     5401 caacaatgct ggctgaagtt agggccacct tcgaacggat tgcgccaaag tctgtggcgc
     5461 ctgacatttg cagcgcagct gcagtgccgc tgatttacag ccggcaagtc ctcggcgtgt
     5521 tagaagtcat cgccattgat ggactgatca tcgatgcaga tcaaattgcg ctgctgcagt
     5581 tactcgcatc acacgctgcc accgctgtga ctcacgcgca attgcacgat cggaatcagc
     5641 aagtgatgag cttgctggag atgattaacg atcgatcggc ggcagtaagc agtgtcggtc
     5701 aagcggtcat caatgctggc catgatcaaa cccgcatgag tttggaagta ttaacccgga
     5761 cgattgcctc attgcatttg gccggtggca aagtcttcct tgaagatcct ataacacacc
     5821 agctagctgg tatcgctagc gagaatctcc ccagggaaga tattgtggcg ggcttggagc
     5881 tagcggcagc ccactgcttg ggtatccgcc agacgatatt gctccagaat atgtccgcgc
     5941 aagcctggac gcgtgaagta acgcagtggt taagcgatca tcatttcggc agactggtct
     6001 gcattccgct catcgccgat aacgaaccag tcggcgtgct gcaagtcgcg gctaagaccg
     6061 atcaagcgat tgaaaccggt atgctggata cactgcacat cattgccgga caactggccc
     6121 tgggtgtggc caatgcgcgc ttgtttaacc gcgttcgcgc cgaacaacag cagctagcgg
     6181 cgatcttgtc cagcagcagc gatgtattga tcgggctgaa tgctaatggc gatattcaat
     6241 tggcgaatcc ggcagctgag cggaccttcg actttgtggc tcaggccgta atcggccagc
     6301 cgctgcgacg cgcaacacag aacgctaccc tcaacaacgc ggttgatagt gccatcaaaa
     6361 gcaagcaacg gcagccgagt ggcatggaaa ttccgctgac cgatgatcaa acgttattct
     6421 gcaatctgtc cccgattatc gatcctctgg ggcaactcgc cggctgggta gcagtaatgc
     6481 aagacatcac ccacttcaaa gaagccgagc gatccaagac cgacatgatc ttaatggcct
     6541 cacatgatct acgcaatccg gtcaatttga ctttaggcgc gctcgatctg ttagggcgca
     6601 gcgccagcaa ctttaacgcc atccagcgcg aagcgtacga attgagtttg ttgggtttgc
     6661 atcgcattga agctttgatc tccaatttgc tcgatctcga acgcatcgag cagcgcatcg
     6721 gcctgaatct gaccaagtgt gatctcaccg agaccgcacg atcggtggtc aatgaaatgc
     6781 gtctaccggc gcaacaacgc gaacagaaat tgtggttaac gctggcctct accggcaaca
     6841 ttccgca
//
LOCUS       DASRTJ010000609         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_283030,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000609 DASRTJ010000000
VERSION     DASRTJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283030"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5835)
                     /locus_tag="VFF70_12500"
     CDS             complement(<1..5835)
                     /locus_tag="VFF70_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45556.1"
                     /translation="MKTTKRSAPRKTPPSIKKKPVERRKPIGAAQPARSRQPDRPIAS
                     SPISELAAPDRSAQVQAALYAIASAASSVQDMQEFYAAMHRIVGQLMYAKNFFIALYD
                     EPSDLLSFPYFVDEKDEVLPPQPLANFRGATGWVLRHGKTIADVDGSWATAKARGEAE
                     EVGTVGEEGIAVPLKIENKTIGVVFIQSYTPGIGYQVGDVAILEFVAQHISTALTRAR
                     AIEETRQRNAELAIINSVQQGLVSKLDFQSIIDLAGDKLREIFGADTTYISLYDPPTN
                     LGHTRYYVDRGQRLYFDPAPAVRGLTAWIFHQGRVLLLGTKEESSAYDAIQLPAGTDH
                     TQDMNESYLGVPIVISAEVRGAISIQSYARHAFDQSHVRLLTALASSLSVALENARLF
                     DETQRLFKAEEQRAAELSIVNSVQEGLASKLDMQSIYDLVGDKIREIFDAQVVIISIF
                     DRVANQSTAYYAIERGVRLPNFTLPIRERILRYLDETHQPLTINTDAVQRSKEFGIEI
                     VPGTESAKALVFAPLIVGDEVKGAISLQNLDRENAFSDSDIRLLQTLASSMSVALENA
                     RLFDEVQTRNQEITETLEQQTATSDILRVIAQSPTDVQPVLDVIARHAVHLIDGVFSA
                     VYRVQGQMIDEVARANFTPSAIEVGRTSYPAPLAWDSSLSSRAVISRGVIHIPDTQNN
                     PDIPEMTKRYAAVQNMQCILFVPMIREGDAIGSIGVGKNDPAPFTDKQITLLQTFAQQ
                     AVIAIENVRLFNELNTRNREITEALEQQTATSEILSVTANSPTDVQPVLDVVAEHSAR
                     LCDSYDALIVQVEGDHYRSIAQWGPVPTPAFDVPLNRDTVTGRAIIDRSIVHVADLLA
                     EPESEFPLSRQFSQQLSGQRTLLAVPMLREGRAIGAIVIRRQEVNPFTDKQISLLQTF
                     ADQAAIAIENVRLFNETKRLLNETEQHAAELAVINSVQAGLASQLEMQSIYDLVGDKV
                     REIFNADTTYIVSYDPDQQLVFSHYYVDKGQRQLPQHIPFGQGIYSRVIQTRQFVLAG
                     TSEEGDQFGATHIPSPDSTDDLNQAALYVPIMLGAEVAGVVSVQSHQPHAYAEKDVRL
                     LTTLANSMSVALENARLFDETQRLLKETNQRAAELSIINSVQQGLASKLDMQSIYDLV
                     GDKIRDIFDARTMIISSYDHAAEMTIFNYSIEKGERYYPTPMPFTGLARHLIRTRQLV
                     LVNEDMETRVSELGMRIVPGTELGKSALFVPLTVGDTVHGGIALYNYDRENAFSDSDV
                     RLLTTLASSMSVALENARLFDETTRLLKETDQRAAELSIINSVQQGLASKLDMQSIYD
                     LVGDKINEIFDVQGVGIMHYNHTTRQIHNPYVIERGQRFYPAPAPFTVFAENMIRTKQ
                     VVVINQDMERQMVDYGMRMVPGTEMAKAIAFVPLIVADQVNGVISLENIDHENAFSES
                     DVRLLSTLASSMSVALENARLFDETQRLLKETDQRAAELAIINSVQQGLASKLDMQSI
                     YDLVGDKIRDIFDAQVVMVLEHDHVQNLSLFRYTIEKGVRLHVEPRPPLGFSAHILKT
                     RQTLVINQDMEQRSLELGSPVIAGDDIKSYLGVPLIVGDEVKGVISLQNIDRENAFSE
                     SDVRLLNTLASSMSVALENARLFDETQRLLKETDQRAAELSIINSVQQGLASKLDVQS
                     VYDLVGEKIREIFDAQAVAIMIFDRRTNLLHYPYIIEKGERIRVDSDPFDDKGFAPYI
                     IRTKQSLMFNTEVAKRAEEFGSYLLGGEEPKSYVGVPLISGDEARGVITLSNIDRENA
                     FTDSDLRLLTTLSSSMSVALENARLFDETTRLLKETDQRAAELALINSVQEGLASKLD
                     VQSVYDLVGDKIRDIFDAQVVQIMAYDRDANRCYWRYMIEKGERQYTDPTEPRGFSSY
                     ILKNAQPLMLNDRINDWWLEHLGTPPTVLKGEMAQSYLGVPLIIG"
BASE COUNT         1337 a         1635 c         1556 g         1358 t
ORIGIN      
        1 gccaatgatc agcggcacgc ccagatacga ttgagccatt tcacctttga gcaccgtcgg
       61 cggagtgccg agatgctcaa gccaccaatc attgattcga tcattcaaca tcaacggctg
      121 ggcgttcttg aggatgtaac tggagaaacc gcgcggttcg gtgggatcgg tatactgccg
      181 ctcacctttc tcaatcatgt aacgccaata acagcgattc gcatctcgat cataagccat
      241 gatctgcacg acctgcgcgt cgaagatgtc gcgaatcttg tctccgacca gatcgtaaac
      301 cgattgcaca tccaacttcg aagcgaggcc ttcctgcacg ctgttgatta acgccagttc
      361 ggccgcgcgc tggtcagttt ctttcagcaa acgcgtggtc tcgtcgaaca ggcgcgcatt
      421 ctccaaagcc acgctcatcg acgacgagag cgtagtcaac agacgcagat cagaatcggt
      481 gaaggcattc tcgcgatcaa tattgctgag cgtgatcacg ccgcgcgctt catccccgct
      541 gatcaacggc acacccacat aggacttagg ttcttcgccg ccgagcagat acgacccaaa
      601 ttcctcggca cgtttggcca cttcagtgtt gaacatcagc gactgtttgg tacggatgat
      661 gtacggcgca aagcctttgt catcgaacgg atctgaatcg acgcgaatgc gttcgccctt
      721 ctcgataatg tacggatagt gcagcaagtt ggtgcgtcga tcgaagatca tgatcgccac
      781 cgcctgcgcg tcgaaaattt cacggatctt ttcgccgacc agatcgtaaa ccgattggac
      841 atccaacttt gaggcgagac cctgctgcac gctgttgatg atcgacagtt cggccgcgcg
      901 ctgatcagtt tccttcaaca gccgctgggt ctcgtcaaac aagcgggcat tctccaacgc
      961 cacactcatg gaagaggcca gcgtgttcag taatcgcaca tcagattcgc taaacgcatt
     1021 ctcacgatcg atattctgga gtgaaataac gcccttcact tcatcgccaa caatcaatgg
     1081 cacgccgagg tacgatttga tgtcatcgcc cgcaatgacc ggcgagccga gctcaagact
     1141 ccgctgctcc atgtcttgat tgatcaccaa cgtttggcgc gtcttgagaa tgtgagccga
     1201 aaagcccaac ggcgggcgcg gttcaacatg caatcgcacg cctttctcaa tcgtgtagcg
     1261 gaacaggcta agattctgaa cgtgatcatg ttcaaggacc atgacgacct gagcatcaaa
     1321 gatatcgcga atcttatcgc cgaccaaatc gtagatcgac tgcatgtcca acttcgaggc
     1381 gagaccttgc tggacactgt tgatgatcgc caactcggcc gcgcgttgat cggtttcttt
     1441 caacagccgc tgcgtttcat cgaacaagcg cgcattttcc agcgccacgc tcatggatga
     1501 ggctaaggtc gagagcaagc gtacgtcgga ttcgctgaat gcattctcat gatcgatgtt
     1561 ttccaaactg atgaccccgt taacttgatc ggcaacgatg agcggcacaa acgcgatggc
     1621 tttcgccatc tcggtgccgg ggaccatacg catgccatag tccaccatct gccgctccat
     1681 atcttgattg atcaccacca cttgtttggt gcgaatcata ttttcggcga agacggtgaa
     1741 cggggccggg gccggataaa accgttggcc acgttcaatc acatagggat tatgaatttg
     1801 ccgtgtggtg tgattgtaat gcatgatccc cacaccttgt acatcgaaga tctcattgat
     1861 cttatcgcca accagatcgt agatcgattg catgtccaac ttcgaggcga ggccctgctg
     1921 gacactgttg atgatcgaca gttccgccgc gcgctgatcg gtttctttca gcaggcgcgt
     1981 ggtctcgtcg aacaggcggg cgttctccaa cgccacgctc atcgacgaag ccagcgtcgt
     2041 caggagacgc acatcagaat cgctgaaggc attctcgcga tcatagttat acaaggcaat
     2101 tccgccgtgc actgtatcac cgaccgtcag aggtacgaac aaagccgact tacccaattc
     2161 ggtgcctgga acgatgcgca ttccaagttc agaaacgcgc gtctccatat cttcgttcac
     2221 caataccagt tggcgcgtgc gaatgagatg gcgtgccaat ccggtaaatg gcatcggagt
     2281 gggataatat cgctcacctt tctcgatgga gtaattaaag atggtcatct ccgcggcatg
     2341 gtcataactc gaaatgatca tcgtccgcgc atcgaaaatg tcacgaatct tgtcgccgac
     2401 cagatcgtag atcgattgca tgtccaactt cgaggccagg ccttgctgga cactgttaat
     2461 gatcgacagc tccgccgcgc gctgattagt ttctttcaac agccgctggg tctcatcgaa
     2521 taggcgcgcg ttctccaacg ctacgctcat cgagttggcg agagtggtca gcaagcgcac
     2581 atctttttct gcgtaagcat ggggctgatg actctggacg ctaaccacgc ctgccacttc
     2641 agcgccgagc atgatcggaa catagagtgc ggcttgatta aggtcgtcgg tgctgtcggg
     2701 ggatgggata tgtgtcgcgc caaactgatc gccctcctca cttgtccccg ccaacacaaa
     2761 ctggcgcgtc tgtatcacgc gcgaatagat gccctgaccg aaggggatgt gctgtggtag
     2821 ttggcgttga cctttatcaa cgtaataatg agagaagaca agttgctgat cgggatcgta
     2881 actgacgata taggttgtat cggcattaaa gatctcgcgc actttatcac cgacgagatc
     2941 gtaaatcgat tgcatttcca actgcgaggc cagtccggct tgcacgctgt tgatcacagc
     3001 caattcggca gcatgctgct cggtttcgtt gagtaagcgc ttcgtctcat taaacaaccg
     3061 cacattctcg atggcaatcg ctgcttgatc ggcaaaagtt tgcaacagcg aaatttgttt
     3121 gtccgtaaac ggattcactt cctggcggcg gatgacgatg gcgccgatag cccggccttc
     3181 acgcagcatc ggcacggcta ataacgtgcg ctggccgctg agctgttgac tgaactgtcg
     3241 gctgagtgga aattcagatt ccggctcggc cagcagatcg gccacatgca cgatcgagcg
     3301 atcgataatg gcgcggccgg tgaccgtatc acgattgagc ggcacgtcga acgccggcgt
     3361 gggcaccgga ccccattgag cgatcgatcg atagtgatcg ccttccactt gcacgatcag
     3421 cgcatcatac gaatcacaca ggcgcgccga atgttctgct accacgtcca gcacgggctg
     3481 aacgtcggtt ggcgaattag ccgtgacgct gagaatctcg ctcgtcgccg tctgctgttc
     3541 cagcgcttcg gtgatttctc gattacgcgt attgagctca ttgaacaagc gcacattctc
     3601 gatggcgatc acggcttgct gcgcaaaggt ctgcagcaga gtaatttgct tgtcggtgaa
     3661 cggcgccgga tcgtttttgc caacgccgat cgagccgatg gcatcgcctt cgcgtatcat
     3721 cggcacgaac aatatgcact gcatgttttg cactgccgca taccgtttgg tcatctcagg
     3781 gatgtcagga ttattctgag tatccgggat gtggatcact ccgcgcgata taacggcgcg
     3841 ggaggagaga cttgagtccc aggccaaagg agcaggataa gatgtgcgcc ctacttcgat
     3901 tgctgaaggc gtgaaattgg cgcgtgccac ttcatcgatc atctgtccct gcactcggta
     3961 tacagcgcta aagactccgt cgatcagatg aaccgcgtgc cgggcaatga catctaacac
     4021 gggctgcaca tcggttggcg attgggcaat cacgcgcaaa atatcgctgg tggcggtctg
     4081 ctgttccaac gtttcggtga tctcttgatt gcgcgtctgc acctcatcaa acaagcgtgc
     4141 attttccagc gccacgctca tcgatgaagc gagcgtctgc aataaacgaa tatccgaatc
     4201 gctgaaggca ttctctcgat caagattttg cagagagatc gcgcctttga cttcgtcgcc
     4261 gacgatcaac ggcgcgaaga ccaatgcttt cgccgattca gtgccgggga cgatttcaat
     4321 gccaaattct ttgctgcgtt ggacagcgtc cgtgttgatc gtcagcggct gatgcgtctc
     4381 atcgagatat cgcaagatgc gttcacgaat cggcagggtg aaattgggca agcgcacacc
     4441 gcgctcgatc gcataataag cggtggattg gttcgccacg cgatcaaaga tactgataat
     4501 caccacctgc gcatcaaata tctctcgaat cttgtcgcca accagatcgt agatcgattg
     4561 catgtccaac ttggaggcga gcccttcttg cacgctgtta acgattgaca gttcggcggc
     4621 gcgctgctcc tcggctttga acagacgctg cgtctcatcg aacagccggg cattttctag
     4681 cgccacactt aaactggaag cgagagcggt cagcagacga acatgactct gatcaaaggc
     4741 atgtcgcgcg tagctctgga tactgatcgc gccgcgcact tcagcgctga tgacgatcgg
     4801 cacgcccaaa tatgattcat tcatgtcttg ggtgtggtct gttccggctg gaagttgaat
     4861 ggcatcatac gccgaagatt cttctttcgt tcctaacaac aacacccggc cttgatgaaa
     4921 gatccaggct gtcaaaccac gcacggccgg ggcgggatcg aagtaaagcc gctggccgcg
     4981 atcaacgtaa taccgggtat gccctaaatt tgttggtggg tcatagagcg aaatataagt
     5041 ggtatccgcg ccgaaaatct cgcgcagttt gtcgccggcc agatcgataa tcgattggaa
     5101 gtccagcttc gagaccagcc cctgttgcac gctgttgatg atcgcgagtt cagcattgcg
     5161 ctggcgcgtt tcttcgatcg cacgggcgcg ggtcaaggcc gtagaaatgt gctgcgccac
     5221 aaattcaagg atcgcgacat ccccgacttg atagccaata cccggcgtgt aactttggat
     5281 gaacaccaca ccaattgtct tattctcaat cttgagtggc acggcgatcc cttcttcccc
     5341 caccgtaccg acctcttccg cctccccgcg cgcttttgcc gtggcccaac ttccatccac
     5401 atcggcgatg gttttgccat gacgcaagac ccaacccgtc gcgccgcgga aattcgccag
     5461 cggctgaggg ggcagcactt catccttttc atcgacaaag taagggaaac ttaacagatc
     5521 cgacggctca tcgtagagcg caatgaagaa attcttggcg tacatcaact ggccgacaat
     5581 gcgatgcatg gccgcataaa actcctgcat gtcttgaacc gagctggcgg ctgacgcaat
     5641 ggcatacaac gccgcctgaa cttgggcgga tcgatcgggc gcagctagct cgctgatcgg
     5701 actggacgcg atcggtcgat cgggctggcg ggatcgagcg ggttgagctg cgccgatcgg
     5761 tttacgccgc tcgaccggtt tcttcttgat cgacggtggt gtttttcgcg gagcagatcg
     5821 cttagtagtt ttcatctgta cgtactccaa tgatttaaga tgatcaggcg caggccgcct
     5881 atcatc
//
LOCUS       DASRTJ010000610         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_206005,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000610 DASRTJ010000000
VERSION     DASRTJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206005"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1401)
                     /locus_tag="VFF70_12505"
     CDS             complement(10..1401)
                     /locus_tag="VFF70_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZY45557.1"
                     /translation="MSIDTLVFFEHLFFWFALGSILLFVPYFLYSKHIHLFIAPLNFM
                     LKPERKSIGQLDKIDFDDQSIEIFGASKLEQMTQTALLDAYACIMCNRGQDVCPASAT
                     GKVLSPSALEINKRYQLNQEGAAIAAGKESTSTLIEFAISPEAVWACTTCGACVEVCP
                     VNNEPMRDILDIRRSMVLMDNSFPTQFQAAFKGMERSGNPWGISPDSRLDWAKGLNVP
                     TIDQNPDPDILWWVGCAPATDARAQKTARDFAKILNAAGVNFAVLGKLGRCTGDSARR
                     SGNEALFFELATGNVETLNEVKPKRIVATCPHCLHTLANEYPAFGGNYDVIHHTQLIE
                     ELLAQGKLQLHGTQTSELTFHDPCYLGRHNDVYDAPRQVLYSVGAEIVEMDRNRNNSF
                     CCGAGGAQMWKEEEHGTERVSTNRIKEAMATGKDTLAVGCPFCMIMLTDAAKAEQTTM
                     QIKDVAEIVAERI"
BASE COUNT          373 a          481 c          402 g          361 t
ORIGIN      
        1 cttcgtggat catattctct ccgcaacaat ctccgccaca tccttgatct gcatcgtcgt
       61 ctgctcggcc ttggctgcgt cggtcaacat gatcatgcag aatggacagc caaccgccaa
      121 cgtatccttg cccgtcgcca tcgcttcttt gatgcgatta gtgctgacgc gctccgtgcc
      181 gtgctcttct tctttccaca tctgcgcgcc gcccgcgccg cagcaaaacg aattgttgcg
      241 attgcgatcc atctcaacaa tctctgcgcc gaccgaatac agcacttggc gcggcgcatc
      301 gtagacatcg ttgtgccgac cgagataaca cggatcgtgg aaggtgagtt cggaagtttg
      361 tgtgccgtgc agttgcaact tgccctgcgc cagcaactct tcgatcaact gcgtgtgatg
      421 aatcacatcg tagttgccgc cgaaggccgg atactcattc gcgagcgtgt gcaagcaatg
      481 cggacatgtc gccacaatcc gcttcggctt gacttcattc aacgtctcga cgttgcccgt
      541 cgccaattca aagaacaacg cttcgttgcc cgatcgccgc gcggagtcgc cggtgcatct
      601 tcccaacttg cccaacaccg caaagttcac gcccgccgca ttgagaatct tggcgaagtc
      661 acgcgccgtc ttctgcgcgc gcgcatcggt cgctggcgcg cagccgaccc accacaaaat
      721 atccggatcg ggattctgat cgatcgttgg cacattcaat cccttcgccc aatcgagtct
      781 cgaatcaggt gagatgcccc acgggttacc cgatcgttcc atgcccttga aggccgcctg
      841 gaattgcgtc gggaatgaat tgtccatcaa caccatactg cgccggatat cgaggatgtc
      901 gcgcatcggt tcgttgttga cggggcacac ctcgacgcac gcgccgcagg tcgtgcaggc
      961 ccacaccgct tcagggctga tcgcaaattc gatcaaggtc gatgtcgatt ctttgccagc
     1021 cgcgatcgcc gccccttctt gattaagttg atagcgcttg ttgatctcca acgccgaggg
     1081 cgacagcacc ttgcccgtcg cactcgcggg acagacatcc tgaccgcgat tgcacatgat
     1141 gcacgcgtac gcatcgagca gcgccgtctg cgtcatctgt tccaacttcg atgcgccgaa
     1201 aatctcgatc gattgatcat cgaaatcgat cttgtccagc tgcccaatcg atttgcgttc
     1261 cggcttgagc atgaaattca gcggcgcgat aaataggtga atgtgtttgg agtagaggaa
     1321 gtagggaacg aagagcaaga tcgatccgag cgcgaaccag aaaaagagat gttcaaagaa
     1381 tacgagcgta tcaatcgaca ttccctgcca ttgcataaca ggcgaaacgt atccccaaat
     1441 ctttcccaat gaattagcga atggttgcca ttgaccaata ttagtgcacg ttattggtat
     1501 gaatgaattc ggtggtaaac aactgatcgg agacaatgcc acacgaatgc tctcgcccaa
     1561 aaaccttgac cccacatgaa acaaaatgaa gaagccaaca atcaacgaat cgcgctt
//
LOCUS       DASRTJ010000611         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_130374,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000611 DASRTJ010000000
VERSION     DASRTJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130374"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFF70_12510"
     CDS             complement(<1..809)
                     /locus_tag="VFF70_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZY45558.1"
                     /translation="MQLALDGIRVIDLTEALAGPYCTMILGDLGADVIKIERPGAGDQ
                     SRIWGPPFIGTESAYFLSVNRNKRSAVIDITSLEGQSLMRKLLEKADVFVCNVRRIES
                     LQKVKLDPETVQAYNPRLIYCSITGYGRTGPYAGRGGYDLVAQGEAGLMAATGEIDGE
                     PMRWPVAIADMTSGVWSATAILTSLYVREKTGQGQFIDQALLDGQVSWAPVMASEYFA
                     TGKRPPRLGNRHANIVPYQVFKTRDKHMIIAVGTPAHWDRFCEMLNLGNMRT"
     gene            complement(832..1464)
                     /locus_tag="VFF70_12515"
     CDS             complement(832..1464)
                     /locus_tag="VFF70_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45559.1"
                     /translation="MSALDKIAYALDRRDEVPNQELATALAAKKDRRGIQEIADNLWN
                     KDKNIQSDCLKVLYEIGFINPTLIADYAPDFIKLLSGSNNRLIWGAMISLSTVADIRA
                     DEVFKHRSEIVREMETGSVITVDNAVKVLALAASHNSKFSGVLFPILLKHLQTCRPKD
                     VPQHAEKSLPAINSRNKDQFVKVLEKRMIDMTGSRAARVKKVVKQANVTR"
     gene            complement(1596..2270)
                     /locus_tag="VFF70_12520"
     CDS             complement(1596..2270)
                     /locus_tag="VFF70_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45560.1"
                     /translation="MNPDSSVLVSVMTKPHDLDLARDQHWYRIPQKSAPKFFPPDFVA
                     FYCTKAFGGEAYSIKWYAQVRGHELLKRRDLIPDQVDHPHADQLYYKLQLGPLLELPH
                     VIPSKHWRRLTFVLTSGDRLFSAWEINDLILGPREDDLMWRALKDAGITAERGYAIRE
                     ERARYNVDLAIPCAKGTVGIVIRERSPRYSAESVLYFKPKQITDDLSSCVSLVRKTIV
                     KYGGAR"
     gene            complement(2443..>2815)
                     /locus_tag="VFF70_12525"
     CDS             complement(2443..>2815)
                     /locus_tag="VFF70_12525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45561.1"
                     /translation="PNTLDQLSIAPELLGELKCGAEYSLIAATFVDKQPVSFCYVASE
                     TESLWDISIDTLSGSRRKGYAALCVAHMIRHMQAQGKQPVWQAVEENPASWRLAQKLG
                     FVPVDELALFEPHEQASSLHS"
BASE COUNT          609 a          772 c          756 g          678 t
ORIGIN      
        1 gttcgcatgt tgccaagatt cagcatctcg cagaatcgat cccaatgcgc cggtgtgccg
       61 acggcgatga tcatatgttt gtcacgcgtt ttgaaaactt gatagggcac gatgttggcg
      121 tgtcgattgc ctaaacgcgg cgggcgtttg cccgtcgcaa aatattcgct cgccatcacg
      181 ggcgcccaac tgacttgtcc gtccaacaat gcctgatcga tgaactggcc ttgtccggtt
      241 ttctcgcgca catacaaaga agtcagaatc gctgtcgccg accacacgcc gctggtcata
      301 tccgcaattg ccaccggcca acgcatcggc tcgccatcga tctcgcccgt cgccgccatt
      361 aatccggctt cgccttgcgc cacgagatca tagccacccc gcccagcata tggcccggtt
      421 cgaccatagc ccgtgatcga acagtagatc aagcggggat tgtaggcctg cacggtttct
      481 ggatcgagtt tcactttttg cagcgattcg atccgccgca cgttgcacac aaacacgtca
      541 gccttctcca gaagcttgcg catcagcgat tggccttcga gcgacgtgat atcgattacc
      601 gcgctgcgtt tgttgcggtt gaccgataga aagtatgcgc tctccgtgcc gatgaacggt
      661 ggtccccata ttctcgattg atcacccgcg cccggtcgtt cgatcttaat cacatccgcg
      721 ccgagatcac cgaggatcat cgtgcagtat ggaccggcca gtgcttcggt taaatcgatg
      781 acgcgaattc catccaatgc aagttgcatt tatactcctt tgataataaa gtcatcttgt
      841 cacattcgct tgtttgacaa ctttcttcac gcgtgccgcg cgtgaaccgg tcatatcaat
      901 cattcgtttt tctaacactt taacgaattg gtccttgttg cgactattga tcgcaggcag
      961 gctcttctcg gcatgctgcg gcacatcttt cggccgacag gtttgcagat gctttagcaa
     1021 aattgggaat agcacgccgg aaaatttact gttgtgtgat gcggccagcg ccagcacctt
     1081 gactgcatta tcgacggtga tgaccgagcc cgtttccatt tcccgcacaa tttccgatcg
     1141 atgtttgaac acttcatccg ccctgatgtc cgcgacggtc gaaagactga tcatcgcgcc
     1201 ccagatcaat cgattgttgc tgccggagag aagtttgatg aagtccggtg cataatcggc
     1261 gatcagtgtc ggattgatga aaccgatctc gtataaaacc ttcaggcaat cactctgaat
     1321 gttcttgtcc ttattccaga gattatcggc aatctcctgg atccctcttc gatctttctt
     1381 cgccgccaat gctgtggcta attcttgatt aggcacctcg tcacgtcgat cgagtgcata
     1441 agcaatttta tctagcgcgg acatgagttg atcctttttg aaaacgtcga ttgtcattcc
     1501 gagcgtggcg aagaatctct atccccgttg aataggttac cggctatggc agagattcct
     1561 cggcgctgcg cgcctcagaa tgacgttgaa ccttgtcacc tcgccccgcc atacttaacg
     1621 atggttttgc gaacaaggct cacacatgag ctgagatcgt cagtgatttg cttcggcttg
     1681 aaatagagca cgctttcggc tgaataacgc ggcgatcgct cgcggatcac aatgccgacc
     1741 gtgcctttgg cgcacgggat cgccagatcg acgttgtagc gggcgcgttc ttctcgaatg
     1801 gcgtagccgc gctcggcagt gatgcccgca tctttgagtg cgcgccacat tagatcatcc
     1861 tcgcgcggac cgaggatgag atcattgatc tcccacgcgc tgaacaatcg atcgcccgag
     1921 gtaagcacga atgtcaaacg ccgccaatgc ttcgatggga tcacgtgcgg cagttcgagc
     1981 agcggaccaa gttgcagttt gtagtacagc tgatcggcgt gcggatgatc gacttgatca
     2041 ggtatgagat cgcgccgctt caataattcg tggccgcgca cttgtgcata ccatttgatc
     2101 gaataggctt cgccgccaaa cgctttggtg caataaaacg cgacgaaatc gggcgggaaa
     2161 aatttcggcg cggatttttg cgggatgcga taccagtgct gatcgcgcgc cagatcgaga
     2221 tcgtgcggct tagtcatgac ggagacgagg acggatgagt cggggttcat gcgtcgattg
     2281 tagcgtgaat tggagtgaga gacaaggcga ggaaatgttc cgtgttgttc aacttgaggg
     2341 gctaaccggt tgagctcagc ggcgcgagcc gcatcgagac tgtaacttgg ctgggccgcg
     2401 tgcggcgaag cgtcggctgc atggcggggt taggcgctca gcttaggagt ggagtgagct
     2461 agcctgctca tgtggctcga acagcgcgag ctcatccacc ggcacgaacc caagcttctg
     2521 tgccagccgc cacgaagccg gattttcctc cacagcttgc cagacgggtt gctttccttg
     2581 ggcctgcatg tgtcgaatca tgtgcgcgac acacagggcc gcatagcctt tgcgacggga
     2641 ccccgacaat gtgtcaatgg aaatatccca cagggattcc gtttcggatg ccacatagca
     2701 gaaagagacc ggctgcttgt ccacaaaagt cgccgcaatc aaagaatact cggcaccgca
     2761 cttgagctcc cccaaaagtt ctggcgcgat agaaagttga tcgagtgtgt tcgga
//
LOCUS       DASRTJ010000612         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_206135,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000612 DASRTJ010000000
VERSION     DASRTJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206135"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFF70_12530"
     CDS             complement(<1..1050)
                     /locus_tag="VFF70_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HZY45562.1"
                     /translation="MIDQSDSAKPKPARTCAPKWYDVPDVKWNDWRWQMSNRLNSVEE
                     LDEIIHLTESEKTALSTKGLFRVDITPYFISLIDPNDPHDPVRKQVIPTARELDKFES
                     MMEDSLAEDRHSPVPGLVHRYPDRVLFLVTTQCASYCRYCTRARIVGDPSATFNRKEW
                     DAAIEYLQRTPQVRDVLLSGGDPLTIAPKLLDELLGRLRAIPHIEIVRIGSRVPVFLP
                     MRVDQDLCDMLQKYHPLWLNIHVNHPNEITPELREACDRMSRAGIPLGNQSVLLAGVN
                     DSVNIQRQLVHELVKMRVRPYYLYQMDLIEGGGHFRTTVSKGIEIIEGLRGHTSGYAV
                     PTYCVDAPGGGGKIPV"
     gene            complement(1260..1940)
                     /locus_tag="VFF70_12535"
     CDS             complement(1260..1940)
                     /locus_tag="VFF70_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZY45563.1"
                     /translation="MADVVAAPKRRRPHVMVIAPDRAAARWAQALAAERVMCLVANDL
                     AMATRLLVEEQRQLELLIVDRVLLERQPRALLNMLRDTGHWPVIVPIKSSPQRPTVAD
                     RKRIAAALSLIRPKRPNDRRIRIGELVIDPHRRRARVKKLHWAPLPPVQYQLLFVLAQ
                     HAGKVVGYQQLLREVWGYDGSQSEAQNLLKAHIRLLRRKLGLTAQGEYLKSVRGHGYM
                     LDAPAGRS"
BASE COUNT          480 a          627 c          546 g          468 t
ORIGIN      
        1 caccggaatc ttgccgccgc cgcccggcgc atcgacgcag tacgtcggca cggcgtaacc
       61 gctggtgtgc ccgcgcaagc cttcaatgat ctcgatccct tttgagaccg tggtgcgaaa
      121 gtggccgccg ccttcaatca aatccatttg atacaagtaa tagggccgca cgcgcatctt
      181 gaccagttca tgcacgagtt gacgctgaat gttgacagag tcattcaccc ccgccaacaa
      241 tacactttga ttgcccagcg ggatgcccgc gcgcgacatt cgatcgcaag cttcgcgcag
      301 ctcgggcgta atctcgtttg ggtgattgac atgaatgttt agccacagcg gatgatattt
      361 ctgcagcatg tcgcacaggt cttgatcgac acgcatcggc aggaaaaccg gcacgcggct
      421 gccgatgcga acgatctcaa tatgcgggat ggcgcgcaat cgaccgagta gctcgtccag
      481 cagcttcggc gcaatcgtca gcggatcgcc gccggataac agcacatcgc ggacttgcgg
      541 cgtgcgctgc aagtattcga tcgccgcatc ccattctttg cgattgaacg tcgccgatgg
      601 atcgccgacg atgcgggcgc gggtacaata gcggcagtaa gaggcgcact gcgtcgtgac
      661 gaggaacagc acccgatcag gataacgatg caccaggccg ggcaccgggc tgtgccgatc
      721 ctcggccagc gaatcttcca tcatcgattc aaatttatcc aactcgcgcg ctgtcggaat
      781 aacctgcttg cgaactggat cgtgcggatc attgggatcg atcaggctga taaaatacgg
      841 agtaatgtcg acgcggaaca aacctttggt gctgagggcg gttttttcac tttcggtcag
      901 atgaatgatc tcgtccagtt cttcgacgct gttgagccgg ttcgacatct gccagcgcca
      961 gtcattccac tttacatcgg gcacgtcata ccatttgggc gcgcaggtgc gcgccggttt
     1021 aggtttggcg ctatcagatt ggtcaatcat attcctttaa agcctcactc ccacacaata
     1081 cttcactttt ctgtgcttca ttctacatca cttccttata gtattcggtg aaaagatggt
     1141 gaaaggtaat aaagtgaaat tttagtgaaa tagaattgca ttttcctcta atcgggtccg
     1201 tgcaacaaaa gccgccggtg gaattccggc ggcgcgctga ttaagcccgg ctgaaaattt
     1261 tatgaacgtc cggcaggggc gtccagcatg taaccatgtc cacgcaccga tttaagatat
     1321 tcaccctggg cggtcaagcc cagcttgcgg cgcagcagcc ggatatgcgc tttgagcaaa
     1381 ttctgcgctt cgctttgact gccatcataa ccccacacct cgcgcagcag ctgttgatag
     1441 ccgacaactt taccggcatg ctgagctaaa acaaaaagca gctgatattg aaccggcggc
     1501 aggggtgccc aatgcagctt cttcactctc gcccgtcgcc gatgcggatc gatcaccaac
     1561 tcgccgatgc ggatgcgccg atcattcggc cgcttgggac ggatcagcga cagcgcagcg
     1621 gcgatccgct tccgatcggc cacggtgggt cgttgaggcg aggatttgat cggcacaatc
     1681 accggccagt gaccggtatc gcgcagcata ttgagcagcg cgcgcggctg gcgctcaagc
     1741 aagacccgat cgacaatcaa caattccaac tgacgctgct cttcaactaa caagcgcgtg
     1801 gccatcgcca gatcattggc gaccaaacac atcacccgct ccgccgccaa cgcctgcgcc
     1861 cagcgcgccg ctgcgcgatc cggcgcaatg accatgacat gcggacgtct tcgttttgga
     1921 gcagcaacta catcggccat atgtttcctc aacggcagta tacatcgaat ccaccatcaa
     1981 tcaactttga cagcctcctt gatcgatgta caatcacgcc ctgttgattt gtcatttttc
     2041 atttgtcagt accaattgtt cccatgccga cacccattca acatctcgcc atcgccgatc
     2101 aactactgca cgattcaaca c
//
LOCUS       DASRTJ010000613         6755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_169161,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000613 DASRTJ010000000
VERSION     DASRTJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169161"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFF70_12540"
     CDS             complement(<1..303)
                     /locus_tag="VFF70_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZY45564.1"
                     /translation="MSPSPDFYDLLTNLFQQTARTPDALPSIPPQQWPPNSPEQRSLE
                     SLQAAWIALEARSQAAKEIEAQYRNIFEATSDGLVIYDTATGRVVEANPAACAMHGY"
     gene            complement(585..1400)
                     /locus_tag="VFF70_12545"
     CDS             complement(585..1400)
                     /locus_tag="VFF70_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45565.1"
                     /translation="MLENQLTDEVAVDRREEIVKTEQPGYVSTQEVTRDVAGEHRLQL
                     FQITRIMWMVLGVLEIMLGLRFTLKLMAANPSNGFAGLVYGLTGTFTRPFDALLGTPA
                     NDGSIFEVTTLMAMSVYAILFGFVARLMLLIADRPRARSAARSVLEQTPSSTADISLD
                     RIDHTDQPPIINVDRIDRTVQPELIGVVKLSVDANAPEEDLLIRAYIDPMNQPQIISV
                     VKLPIDVNSPKEDPLIRAYGEILRRYRRSMSISTPVISLNGVELEPQTADVGL"
     gene            complement(2748..2975)
                     /locus_tag="VFF70_12550"
     CDS             complement(2748..2975)
                     /locus_tag="VFF70_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZY45566.1"
                     /translation="MDANKDILQGKWHELKGQARQRFAKLTDDDVERLSGKMEELSGV
                     LQKKYGYDKAKADTEINNWLKEADKKHNAKA"
     gene            3297..4100
                     /locus_tag="VFF70_12555"
     CDS             3297..4100
                     /locus_tag="VFF70_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZY45567.1"
                     /translation="MTLNLKVVTLNLLNDPTYWPLRAPLIVDELKALQPDLIALQEVA
                     LPDNTAQWIADQLGGYSLHLTPKSGGLGRREGLAILSRLPIEAHAGFSFEHQGRVAQR
                     VSVQYAGQPWTFVNAHLYWHPFDDRTRRAEVQTLMEWLPSPAILCGDFNAEPHYASIS
                     MVKRRFQSAYAVANGHEPDYTCPTPLYRGPAPRQAARRAILRLGGWLDRPRSEMWRGT
                     LDYIFVDPAIEVRDCRLAFDQPAQHNRRIYPSDHFGLMATLQAKNEPKA"
     gene            4104..4463
                     /locus_tag="VFF70_12560"
     CDS             4104..4463
                     /locus_tag="VFF70_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZY45568.1"
                     /translation="MDRAVHVTVRFMLLWALQALSLVVMTRLVLGIGLLTSDAWSAFA
                     NAIAAGLILALMNTLVRPSLLRLKLPINWLTIVGSTLFVNVLLLILFGRVLPLLDISN
                     LAAAFLGGLVSRSLMAY"
     gene            4547..6169
                     /locus_tag="VFF70_12565"
     CDS             4547..6169
                     /locus_tag="VFF70_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZY45569.1"
                     /translation="MSEPGRGIVMLEIDGLSYRRLQAALDQGLMPTLRDMLRTTHAVA
                     SYDCGLPSQTSSSQAGILYGDNFDIPAFRWYDKERGKLIVSNNFDDAAEINARYGQGQ
                     GLLRGGSSINNLIAGDAEKSVLTLSTLNTTNEQERSRRAQDLFVFWLNPFLFIRSLVL
                     SLCEILLELGQALRQRLLNVQPRINRLERGYPLLRALTNVFLRDLSTYLVALDVVRGV
                     PAIYTTYLSYDEVAHHAGPDTSDALETLRSLDRQIRRIRKVIAAKAPRPYDLFVLSDH
                     GQSAGATFRQRYGQSLTEFIAAHTASPGIVAEVSSRMIGRSYAAALVAELQTAERVVA
                     GRVRRLVMRGSRRALQKRIKKEQPAVMDAPIVVCVSGNLANVYFNSHSGNVSLSEINT
                     EHPQLVDQLVMQAGIGFVIVYNDESTPLVLGKEGARDLATGLVTGVDPLQPYSLPDLR
                     AQQLWRVAQFPHSGDLIIVSTMYHAEQVAAFEELVGSHGGLGGEQTSAFLLHPADVPV
                     PEMKNSTDVFPVLNARRGQLDERVIDKKLAEV"
     gene            complement(6193..>6755)
                     /locus_tag="VFF70_12570"
     CDS             complement(6193..>6755)
                     /locus_tag="VFF70_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HZY45570.1"
                     /translation="KVLVTTSGMIGGAAGLTTSLIELPVSTCLVLRSIADIARSEGHD
                     ITRPEIKLACLEVLALSGKSDNDEVLGEGYWAVRSFLAKSVSEAAAYITEKGLVEEGA
                     PPLAKLIAKIAARFSTVVTEELAAKAVPIVGAVAGGTINLLFMQHFQAMATGHFIVKR
                     LEGKYGSERVERMYVECDPSVDKTVQ"
BASE COUNT         1457 a         1797 c         1852 g         1649 t
ORIGIN      
        1 atagccgtgc atcgcacagg ccgcagggtt ggcctcgacc acgcggccgg tcgcggtgtc
       61 gtagatgacc aagccgtcac tcgtcgcttc aaaaatattc cggtattgcg cttcaatttc
      121 tttagcagct tggctccgcg cttctaacgc gatccacgcc gcctgaagac tttcgagcga
      181 gcgctgttcc ggcgaattgg gcggccattg ctggggagga atactgggca gtgcgtccgg
      241 ggtgcgagcc gtctgttgaa acaggttggt taacagatca tagaaatcag gcgaaggaga
      301 catcgtgcct accgtgtcat tcgaatactt aattttacta cactgcgcgc ccataagcca
      361 ccggttagat gcacgagaca acgcaagcta atcttaccgc atgttaggcc atctgcgtaa
      421 ctgccacacg ccacatatcg atcagtttga ctcaaagccg cctagtgcta gctgcaatag
      481 atcgatatcg aggtattggc acagcgagat aaagagaaat ccgatcttca ttcagttgaa
      541 gatcggactt ttgtttgatc taatttggcc caccgcttta gcagctagag ccccacatct
      601 gctgtctgag gttccagttc tactccgttg agggagatca ccggtgtaga gatactcatc
      661 gatcgtcgat accggcgcaa gatttcgcca tacgcgcgga tcagcggatc ctccttgggg
      721 gagttcacgt cgattggtaa cttcacgacg ctgatgatct gtggctgatt catgggatcg
      781 atatatgctc ggatcagcag atcctcttcc ggggcgttcg cgtcgaccga taacttcacg
      841 acgccgatga gctccggttg aacggtgcga tcgattcgat cgacattgat aatcggcggc
      901 tgatctgtgt gatcgattcg atcgaggctg atatcggccg tactgctcgg tgtttgctcc
      961 aacactgatc gtgcagcgct tcgagcacgg ggacgatccg cgataagcag catcaggcgc
     1021 gccacaaaac cgaagaggat cgcatacaca gacatggcca tgagggtcgt cacctcgaag
     1081 atcgagccat cattcgcagg cgttcccaac aaggcatcga acggtcgggt gaatgtcccg
     1141 gtaagaccat agactaaacc ggcaaagccg ttgctcggat tggccgccat caactttaag
     1201 gtgaagcgca gccccagcat gatctctaga acgcccaaca ccatccacat gattcgagtg
     1261 atctggaata actgcagtcg gtgctcgccg gccacgtccc gcgtcacttc ttgggtggac
     1321 acatatcccg gttgttccgt tttcactatt tcttcacggc gatccactgc cacttcatcg
     1381 gtcaattgat tctctaacat agtatctcct tttgaactgc aagcctgtca gagtaagtgt
     1441 gttggacgat gctatcgact aaactgctgt tcagactaaa acgactccaa ttgaaattct
     1501 gcaagattag gccgagcgtc gaatggtctt cacgctctgc gtcagttagg agtatgccaa
     1561 gtcttagttc agcataccgt gaataaaccc caactacatc cagcagttgg ggtgtgtcga
     1621 tctccgccag ttgggggaga tagagctggt aagaggggcg cgcgttaggc tacagtctgc
     1681 aatggtgtat caaccggatt aagactggaa gccaaagtta ccttcaacca aatattgaaa
     1741 acgccggact gatcgctctc tccgcgatag agtcataggc gaccccatcg gcctgaaagc
     1801 gccgcggcgg tatcatacga gccacctcag ccttgattga agtttcatca tccatgaata
     1861 ggaattcgtc actccgctca gattctcgga ttgcctgcca gacgtcgtca agtgtgacgg
     1921 tagtcatcct gttgctcctc aaatgtctga tgtattgctg gatcggatcg atttggaatt
     1981 ctgcaacttc agggccgagt gtcgaatagc cttcatgcta tgtgttggcc agaaatatgc
     2041 gaacccagaa ttcatcatac agtcaacaca ccccaactac atccagcagt tggggtgtgt
     2101 ccatctccgc cagttggggg agatggttgg aatttacgca ctggtgattg gcaggctggg
     2161 gttgaaaagg ttatttgtga tcgtgatcga gtgagcgatc acgcttgcgg tggtcagtac
     2221 aagtgcggct atggcgggaa ctcaaaattg ccgtctatca ggtgccgaaa gcgtgggact
     2281 gatcgctcca tccgcgatcg agttagaggc aaccgcagcg gactgaacac gccgcagctg
     2341 tgctaaacga gccgcctcag ccttgattga agtttcgtcg ttcgtgaagt agctttcatc
     2401 actccgctcg gattctcgga ttacctgcca gatgtaatca actgtgactg tagtcatatc
     2461 gtagctcctc aagtgtttga cgtatcgcta gatcagatcg atcagcggcg gtgtggcagg
     2521 ggaaaggttg ctcgatcgat gccgcgctca atatctattg atgataaccg cttcgcacac
     2581 cggtgactat cagcacgctg ctgattaagt tgtcagtgct tggactacac atgcggccag
     2641 tgatccaagg acggcagtct gaaagagttg ctgcaaaatg gccctgcacc acgcagggcc
     2701 attcgcttca tcaggctatt caattttgcg cgacacacga ccggtgttta ggctttggcg
     2761 ttatgctttt tgtcggcctc tttcaaccag ttgttgattt ccgtgtcggc tttggcttta
     2821 tcgtagccat acttcttctg caagacaccg gagagctctt ccatcttgcc gctcagccgc
     2881 tcgacgtcgt cgtcggtgag tttagcaaac cgctgccgcg cctggccttt gagctcatgc
     2941 cacttgcctt gtaggatgtc tttgttcgcg tccatgtgaa tttctccttg attatggtgc
     3001 tacttaattg aacatgtatc gaatgtaacg gacctggccg tcagatattt tgcgcccgag
     3061 cgttacacca tcactcaata gacacaacaa tgtggcttgt tttctgttgc ttcggcgggc
     3121 gaaagtcgaa cgatcgatgc aacactcggt aacatcatac taagcgattc acgcgccgct
     3181 ggctatcagc ctaatgctga ttaagttgtc agtgtttgga ctacaccata cggctattaa
     3241 tagatggcga tgatataatc gttgtgcgga tgcgataaga tttagcttac gctcatatga
     3301 ctctaaatct aaaagttgtc accttaaact tgctgaatga cccaacctat tggccactgc
     3361 gcgcgccctt gattgtcgat gaattgaaag cgctgcaacc cgatctgatt gccctccagg
     3421 aggtggcact gcccgataat acggcgcagt ggatcgccga tcagttgggg ggctactcgc
     3481 tgcaccttac gcccaagtcc ggcggtcttg gtcgacgtga agggttggcg atcttgagcc
     3541 ggctgccgat cgaagcacat gcgggatttt ctttcgaaca tcagggccgg gtcgctcagc
     3601 gggtaagcgt ccaatatgcg gggcagccct ggacctttgt gaacgcgcat ctctattggc
     3661 acccgttcga cgatcggacg cgccgggctg aagtccaaac gctgatggag tggctgccgt
     3721 ctccggctat tttgtgtggc gactttaatg ctgagccaca ctacgcctca atttcgatgg
     3781 tgaagcggcg tttccagtcg gcgtatgctg tggctaacgg acacgaaccc gactacactt
     3841 gcccgacgcc gttgtatcgc gggccagcgc ctcgtcaagc ggcgcgacga gcgatcctac
     3901 gtctcggagg ctggctcgat cggccgcgca gtgaaatgtg gcggggcacg ctcgactaca
     3961 tcttcgtcga tccggcgatc gaggtgcgtg actgccgcct cgctttcgat caacccgccc
     4021 aacacaatcg gcgcatttat ccgtcggatc attttggttt gatggccacc ttgcaagcga
     4081 agaacgagcc gaaggcataa attatggatc gagccgtgca tgttactgta cgttttatgc
     4141 tgctatgggc gttgcaggcg ttgtcgctgg tggtgatgac gcggctcgtg ctgggtattg
     4201 gtttgctaac gagcgatgcc tggtcggcct tcgccaacgc gatagctgcc ggattgatcc
     4261 tggcattgat gaatacactg gtacgcccat ccctgttacg gttgaagctg cctatcaact
     4321 ggttgaccat cgtcgggtcg acgctgttcg tcaatgtgtt gctgctgatt ctcttcggca
     4381 gagtgctgcc actgcttgac atttcgaatc tggcggctgc gtttttgggt gggttggtat
     4441 ctcgatcgtt aatggcgtac tgacgggttt tgtcaacatt agccgtgatg actcgttctt
     4501 tcaaaacgtc gtcgagtaca tcgccaaacg ccaatcgatt aacggggtga gtgaaccggg
     4561 gcgcggcatc gtcatgctgg agatcgatgg cttgagctac cggcggctgc aagccgcact
     4621 cgatcaggga ctgatgccga ctttgcgaga tatgctgcgc acaacgcacg cggtggcaag
     4681 ttacgattgc ggcctgccgt cacagacttc gtccagtcaa gccggtattt tgtatggtga
     4741 caacttcgat ataccagcat ttcgctggta cgacaaagaa cggggcaagc tcatcgtttc
     4801 gaataacttc gatgacgcgg ctgagatcaa cgcacgctac gggcagggcc aagggctgtt
     4861 gcgcggcggt tcgagcatca acaacctgat cgctggcgat gccgagaagt ctgtattgac
     4921 cttgagcaca ctcaatacaa cgaacgagca agagcgcagt cgccgtgcgc aagacctgtt
     4981 tgtgttctgg ttgaatccat ttctgtttat acgcagcctg gtgttaagct tatgcgaaat
     5041 actgctcgaa ctcggtcagg cgctgcgtca acgcttgctt aatgtgcagc cgcggatcaa
     5101 tcgtttggag cggggctatc cgctcttgcg ggcactcacg aatgtgttct tgcgtgacct
     5161 cagcacctat ctggtcgccc tcgatgtggt gcgtggtgtg cctgccatct acacgaccta
     5221 cctcagttac gatgaagtcg cccatcatgc cgggccggat acgtcggacg cgctggaaac
     5281 gctgcgcagc ctggatcgac agatccgccg catccgcaag gtgatcgccg ccaaagcacc
     5341 gcgtccgtat gatttgttcg tactatccga tcacggtcaa tccgcaggcg cgaccttccg
     5401 gcagcgctat ggtcaatcgt tgacggagtt tatcgcagcg catacggcgt cgcccgggat
     5461 tgtggccgag gtgtcaagtc gaatgattgg taggagctat gccgcggcgt tggtcgccga
     5521 attgcagact gccgagcggg tggtagccgg tcgtgtgcga cggttagtca tgcgcgggtc
     5581 acgccgggct ctgcagaaac gtattaaaaa ggaacaacct gcagtgatgg atgcgcccat
     5641 tgtcgtgtgt gtctcgggca atctggcgaa tgtatacttt aactcgcata gcggcaatgt
     5701 cagtctgagc gagatcaaca cagagcatcc acaattagtc gatcagctgg tgatgcaggc
     5761 cggcatcggt tttgtgatcg tgtacaacga cgaaagcacg ccgctcgtat taggcaaaga
     5821 gggcgcgcgt gatttggcga cgggtcttgt caccggcgtc gatccactcc aaccgtattc
     5881 cctgcccgac ctacgcgcgc aacagctctg gcgagtcgca cagtttccac attcaggtga
     5941 tctcatcatc gtcagcacga tgtatcacgc tgaacaagta gcggcttttg aggaattggt
     6001 tggcagccac ggcggtctcg gcggcgagca gaccagcgct tttctgctgc atccagccga
     6061 tgtgcctgtg ccggaaatga aaaactccac cgatgtcttt ccggtgctca atgcgcggcg
     6121 cggtcaactc gacgagcgcg tgattgataa gaaacttgca gaagtttgat cagaagcttg
     6181 ggcagatcat gcctattgaa cagtcttatc gactgatgga tcacattcta catacattct
     6241 ctcgacccgc tctgagccat atttcccttc gagtcgcttg acgatgaaat gtccggtggc
     6301 catcgcttga aagtgctgca tgaatagcag attgatcgtg ccaccggcca cagcccccac
     6361 aattggcacg gctttagccg ccagctcttc ggtgaccacc gtgctaaatc gggcggcgat
     6421 tttggcgatt aatttggcca gcggtggcgc gccttcctcg accaagcctt tttcagtgat
     6481 ataggccgcc gcttccgaaa ctgatttcgc cagaaaactc ctcacggccc aatagccttc
     6541 acccagaact tcgtcattat cactcttgcc gcttaacgcc agaacttcca ggcaggctaa
     6601 tttgatttca ggtcgagtga tgtcgtgtcc ctcactgcgc gcaatgtcgg cgatcgatcg
     6661 caagactagg caggttgaga cgggcaattc gattagcgat gtggtgagac cggcggctcc
     6721 gcctatcatt ccggatgtcg tgactaacac cttgt
//
LOCUS       DASRTJ010000614         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_206819,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000614 DASRTJ010000000
VERSION     DASRTJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206819"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VFF70_12575"
     CDS             complement(<1..1294)
                     /locus_tag="VFF70_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZY45571.1"
                     /translation="MSVLTAHHLSKSYGAFDVLGDVTLALAHNQRAALVGPNGVGKTT
                     LLHLLAGIEEPSGGAIHRARGQTIGFLPQHADQLLSEAESLHDLMRSVFAHLDQQAAQ
                     LRQLEHDMAHPDRPDEVIEKYGKLVEQFEHAGGYTFENRIEQVLSHVGFERSDFDKPV
                     NILSGGQKTRALLARLILQAPDVLLLDEPTNHLDVSAVEWLEATLKDYPGAIIVVSHD
                     RYFIDAVADTVWELKAGTIEIYSGNYTRYLIQREDRFERQLKEFEKQQEFMAKEEEYI
                     RRNLAGQNTRQAQGRRTRLERLRKSADLIKQPAARQAMKLNLSAERRSGDIVLHARDL
                     VVGYPDDRKPLLSCAELFLYRGQRAAIWGPNGAGKSTFLKTVLGQLPPLSGEIELGAS
                     IKVGYYAQAHELLDPNDSVLHSMLKAQPNMTVSKARSWL"
BASE COUNT          349 a          433 c          429 g          386 t
ORIGIN      
        1 ccaaccaact acgtgctttc gagaccgtca tgttcggttg tgctttaagc attgaatgca
       61 gcaccgaatc atttggatcg agcaactcat gcgcctgcgc gtaatagccg actttaatgc
      121 tggctccgag ttcaatctcg ccgctaagtg gcggaagttg gcctagcact gttttcaaga
      181 aagttgattt gcccgcgcca ttcggacccc aaatggcggc gcgctgccct cgatataaaa
      241 ataattcggc gcatgacagc agcggcttgc gatcgtctgg ataaccgacg acgagatcgc
      301 gtgcgtgcaa aacgatatcg ccgctgcgcc gctcggctga aagatttaac ttcatggctt
      361 ggcgcgcagc gggttgcttg atcaagtcag ccgacttgcg taatcgctcc aggcgagtgc
      421 ggcggccctg cgcttggcgc gtgttttgtc cagcgagatt acgccgaatg tattcttctt
      481 ccttcgccat gaactcttgc tgcttctcaa attctttcag ttggcgctcg aagcgatctt
      541 cgcgctggat taagtaacgg gtgtagttgc cggaatagat ttcgatcgtg cccgccttca
      601 attcccacac cgtgtcggcc accgcatcga tgaaatatcg atcgtgcgac acgacgatga
      661 tcgcgcccgg ataatctttc aaagtcgctt ccagccactc gacggcactc acatccaagt
      721 gattggtcgg ttcgtcgagc aacaagacgt cgggcgcttg caagatcaag cgagccaaga
      781 gcgcgcgcgt tttttgtccg ccgcttaaaa tgttaacggg tttatcaaag tcgctgcgtt
      841 cgaagccaac gtggcttaag acttgttcga tacgattctc gaaggtgtag ccgcccgcat
      901 gctcgaactg ttcgacaagc ttgccgtatt tctcgatcac ctcgtcaggc cgatcaggat
      961 gagccatatc gtgttcgagt tggcgcagct gcgcggcttg ttgatcgagg tgcgcgaaga
     1021 ccgatcgcat gaggtcgtgc agcgattcgg cttcagacaa aagttgatcg gcatgctgtg
     1081 gcaggaaacc gatcgtttgg ccgcgcgccc gatggatcgc cccgccgctg ggttcttcga
     1141 tgccggccaa tagatgcagc agcgtggtct tgcccacgcc gttcgggccg accagcgccg
     1201 cgcgttgatt atgcgccagc gccagcgtca catcgccgag gacgtcgaac gcgccgtaag
     1261 atttggagag atgatgggca gtgagaacgg acatgaatct aataagtaac gagtaatgat
     1321 ttattttgct ttgcaaatta tacatcacaa cccagcatga tcactcgtcc actcggtata
     1381 tttgaaaatt attttcatca tcaatcgatg cagattactc gtcaatcaat actcgttatt
     1441 tttacggtat aataccccgc atgaatgcag aacctcggcg tatcacccct cgatcggaaa
     1501 tgcgatccag cggcaatgtc atcttcgagt tgatcagcgc gctcggactc atcgtgctgg
     1561 cggggatcgg cgcatcgctc gcgatggaag cgcgaat
//
LOCUS       DASRTJ010000615         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_283983,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000615 DASRTJ010000000
VERSION     DASRTJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283983"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VFF70_12580"
     CDS             complement(<1..1750)
                     /locus_tag="VFF70_12580"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HZY45572.1"
                     /translation="MTNRIQQLSQLGQSIWYDNIQRKMIASGKLKAMTDEGLLGMTSN
                     PTIFEKAIAGSADYDASINTMIDEGRSTEGIYDALTIEDVGLAADVFKPVFDRTKGVD
                     GYVSIEVSPKLAHDSAGTLTDARRLWKTLKRSNVMVKIPATKEGLPAIEQALTDGINI
                     NITLMFSMEHYEAVAEAYLCALEKRMKTGKRIDHIASVASFFVSRVDTLVDAKLAEII
                     RQEGSNAALAASLQGKVAVTNSQVVYARFLQIFNSDRFKKLQAKGAKVQRVLWASTST
                     KNPKYPDTIYVNTLIGPDTINTVPPETYDAIKDHADVKRTVDADLAGAHKVLNDLQSL
                     NIHMNDVGEQLSVEGVDKFTKSFDQLLIVLDAKRMSLMAQHIGRGSAALGQYQAAIDQ
                     RLAAFDQSDLTKRVWAIDASVWSTEPEHVQEITNRMGWLTVIEDMHTHAADLRAFADE
                     VRSTGFKDAVVLGMGGSSLGPDVARLTFGSAKGYLKLHVLDTTNPDSIAALEKQINPL
                     KTLFIASSKSGTTIEVESLHRYFRNKLYPKLSEAYGKNFIAVTDPGTPLAALAQKENF
                     RRAFINQPDIGGRYSVL"
BASE COUNT          420 a          500 c          497 g          497 t
ORIGIN      
        1 acagcaccga atagcgcccg ccgatgtcag gctgattgat aaacgcgcgg cggaaatttt
       61 ctttctgggc caacgctgcc agcggcgtgc ccggatcagt caccgcaatg aaattttttc
      121 cgtatgcctc gctgagtttt ggatacagtt tgtttctaaa atatcggtgc agtgattcca
      181 cttcgatcgt cgtgccggac ttgctggaag cgatgaacaa ggtcttcagc ggattgattt
      241 gtttttcgag cgcggcgatc gaatcgggat ttgtcgtgtc taacacatgc agcttgagat
      301 aaccctttgc gctgccaaat gtaaggcgcg ccacatcggg gccgaggctg ctgccgccca
      361 tgcctaacac gaccgcatct ttaaagccgg tcgatcgaac ttcgtcggcg aacgcgcgca
      421 gatcggcggc gtgagtgtgc atgtcttcga tcaccgtcag ccagcccatt cgattcgtga
      481 tctcttggac atgctccggt tcagtcgacc acacactcgc gtctatggcc cacacgcgtt
      541 tagtcaaatc ggattgatca aaagccgcta atctttgatc gatggccgct tgatactggc
      601 ccaacgcggc tgagccgcgc ccgatgtgct gagccatcaa actcattcgt ttagcgtcca
      661 acacaatcag caactgatca aacgatttag taaacttgtc tacgccttca accgacaatt
      721 gctcgccaac gtcattcatg tgaatattaa gagattgaag atcgttcaac actttgtgcg
      781 cgccagcgag gtcggcatcg accgtgcgtt tgacgtcggc gtgatctttg atcgcatcgt
      841 aggtttccgg cggaacggtg ttgatcgtat ccgggccgat cagcgtattg acgtagatcg
      901 tgtcaggata tttcggattc ttggtgctgg tgctggccca caatacgcgc tgaactttcg
      961 cacctttggc ttgcaatttc ttgaatcgat cgctattgaa gatttgcaga aaacgcgcat
     1021 agaccacttg cgaattcgtg acggcaactt tgccctgcag cgaggcggcc agcgctgcgt
     1081 tcgatccttc ttgacggatg atctcggcca acttcgcatc caccagcgta tcgacacggc
     1141 tgacaaagaa cgacgccacc gaagcgatgt gatcgatccg cttgccggtc ttcattcgct
     1201 tctccaacgc gcacaggtag gcttcagcta ccgcttcata gtgttccatt gaaaacatca
     1261 aggtgatgtt gatgttgatg ccatcggtta acgcctgctc gatcgcgggc agaccttctt
     1321 tcgtcgccgg aattttcacc atgacgttgg atcgtttgag tgtcttccat aagcggcgcg
     1381 catcggttaa ggtgcccgct gaatcatgcg ccagcttggg cgatacttcg atactcacgt
     1441 acccatcaac gcctttggtc cgatcgaata ccggcttgaa cacatcggcg gccaggccca
     1501 catcttcgat cgtgagcgca tcatagatgc cctccgtcga tcgaccctcg tcgatcattg
     1561 tgttaattga tgcgtcgtaa tcggcgctgc ccgcgatcgc tttttcgaag attgtcgggt
     1621 tggaagtcat gccgagcaag ccttcatcgg tcatcgcttt gagtttgccg gacgcgatca
     1681 tcttgcgttg gatgttatcg taccaaatgc tctggccgag ttgtgagagt tgttggatgc
     1741 ggttggtcat aagagctttc cttgtgttga tttagattaa ttggtaagaa agagagatag
     1801 agagaatgag agatcaacct tcggcgataa ccgattttag agattccaat cttttgaatg
     1861 agtagaacaa aagcagattg atagcgacga ctgcgccatg cctcagcaat atta
//
LOCUS       DASRTJ010000616         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_130743,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000616 DASRTJ010000000
VERSION     DASRTJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130743"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1034)
                     /locus_tag="VFF70_12585"
     CDS             complement(180..1034)
                     /locus_tag="VFF70_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45573.1"
                     /translation="MTNIRKFRLGQASITVINTGDLQLKMADWLKVSPEVWSQFYPID
                     LYNEPLHLPTQCIHIKIGRTSILIDANVYDVSADSSYAIPNYQPPLDLIASLIESGIR
                     PEDVTHVIITHAHFDHYNGTTTQRNGILVPKFANALYYLGRADWDDPDTQTALKEKGS
                     LQHRTLGVLHDHAVLELVEGDRDLIEGVSIIAAPGESPGHQIVRVHSDDQTLYCVGDL
                     YHHPAEAEHPTWHANWADAESILASRDAFTSRALAENAIVIATHIPSIGRLQQTPSGV
                     NWIEAEMT"
     gene            complement(1081..>1677)
                     /locus_tag="VFF70_12590"
     CDS             complement(1081..>1677)
                     /locus_tag="VFF70_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45574.1"
                     /translation="TISKNWWPDDIFRSIDLKCEREAWGQWSIKDSYAVLDLGEFGFT
                     KLFDACWIYLEAANFRPAVDSRPLRFEIANSEEALSAWRLAWDSDERLGKEIFDSKLL
                     DNKRVYFIAGYKAEQIVSGCFVNKTDNVLGISNFFSPNEGVDHWSAIISFIFSSIEHS
                     DLVGYERCERGDKLQSLGFESIGNLTVWLKKRSDTGRG"
BASE COUNT          412 a          427 c          402 g          436 t
ORIGIN      
        1 tcacagccac tgcgcccata acaccaacga agtaacctcc atcgactgaa acaccacgcg
       61 gcggctctcg atcgagagcc gccgcgtggt cttgcacaat ctttgtgaag catcctatgc
      121 gaatacctca cccgccatgc gagtgagact gaagtcgaag gatatacgca ctactccgct
      181 catgtcattt cggcctctat ccaattaacg cctgatggag tttgctgcaa tcgcccgatt
      241 gatgggatat gcgtagcaat aacgatcgca ttttcggcca gcgctcttga agtgaaggca
      301 tcgcggctgg caaggatcga ttcagcgtcg gcccagttgg cgtgccaggt tggatgttcg
      361 gcctcggcgg gatggtgata caaatcaccg acacagtata aagtttgatc gtccgaatgc
      421 actctgacga tttgatgtcc cggcgattcg cccggcgcgg cgatgatcga gacgccttcg
      481 atcagatcgc ggtcaccctc aacgagttcc agcacggcgt gatcgtgcaa cacacctaac
      541 gttcgatgtt gtaatgaacc cttttccttt aaagccgttt gcgtatcggg atcgtcccaa
      601 tcggctcgac cgaggtaata gagggcgttc gcaaatttcg gaacgaggat tccgttgcgt
      661 tgggtggttg tgccattgta atgatcgaag tgcgcgtggg tgatgatgac atgcgtaacg
      721 tcttctggcc ggatgcccga ttcaattagg ctcgcgatca agtctagtgg cggctgatag
      781 ttcgggatcg cgtaagagct gtcggccgaa acgtcgtaga cgttagcatc aatgaggatc
      841 gaggtgcggc cgatcttgat gtgaatacac tgcgtcggca aatgcagcgg ctcattgtac
      901 aaatcgatcg ggtaaaactg cgaccaaact tccggcgaga ctttcagcca atctgccatt
      961 ttcaattgta gatcacccgt gttgatgacg gtgatggatg cttggcctaa tcggaatttg
     1021 cggatgttag tcattattga agtggaatgc tctgcgctga gtcatttaac acctcgcggt
     1081 tcagccgcgc ccggtatcgc ttcttttctt taaccacaca gtcagatttc ctattgactc
     1141 aaatcccagt gattgaagtt tgtccccccg ctcacatcgt tcataaccta cgaggtctga
     1201 atgttcaatg gagctaaaga taaaactgat tatagcagac cagtggtcaa caccttcgtt
     1261 gggcgaaaag aaattcgaga tgcctaaaac attgtcggtc ttattgacaa aacatccgct
     1321 aacgatctgt tctgccttat atccggcgat gaaatatact cgcttgttgt ctaataattt
     1381 tgaatcgaat atttctttac caaggcgctc gtcagaatcc caggccaatc tccacgcaga
     1441 caaagcctct tcgctatttg cgatttcaaa acgtaatggt cggctgtcta ctgctggcct
     1501 gaaatttgcg gcttcaagat atatccagca cgcatcaaat agttttgtaa atccaaattc
     1561 acccaaatct aagacagcat agctatcctt aattgaccat tgcccccatg cttccctttc
     1621 gcacttgaga tcgatgctcc taaaaatatc atcaggccac cagtttttag aaatcgt
//
LOCUS       DASRTJ010000617         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_207009,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000617 DASRTJ010000000
VERSION     DASRTJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207009"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFF70_12595"
     CDS             <1..503
                     /locus_tag="VFF70_12595"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZY45575.1"
                     /translation="SVLRFAPLIFVVALIYPLGRLGWDYLQWSAEQYLVTSHRVIQTE
                     GLINKRTKDSSLEKVNDIQLTQSFFGRLLGYGELEIVTGSEEGVNILHRLNNPVSFKT
                     ALLDQKVALGQNAGGDQVRPGGAAGTAGGDDSLKRLGDLDDLRKRGAISDQEYQEAKA
                     KILGKL"
     gene            561..1400
                     /locus_tag="VFF70_12600"
     CDS             561..1400
                     /locus_tag="VFF70_12600"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZY45576.1"
                     /translation="MSDTITREQIDEAVALIRQHTAQLPEVGIVLGSGLGELAAAVER
                     PDIIPFADIPHFPISSVLGHAGRMLIGQLEGRSVLVMQGRAHFYEGYSMGQVTFPIRL
                     MQFLGIKTVILTNAAGGLNPSFNSGDVMLITDHINLIGMAGNNPLRGPNDESFGTRFP
                     DMSEPYDKQLGSIALQTAAQNKIDLRQGVYMCLAGPSFESPADIHFLRMIGADAVGMS
                     TVPEAIVARHGGLRVLGLSGISNSLPPAGVKQETTHAEVLAAGRTLVPKMITIIRGVL
                     RSV"
     gene            1406..2170
                     /locus_tag="VFF70_12605"
     CDS             1406..2170
                     /locus_tag="VFF70_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45577.1"
                     /translation="MVFFVQLLANYAGLIYIGCAIGALIYLRDILAARSEIQTSLYSL
                     EREGASSRVMRGSIMVVLFIGVAIVAYILANVVAPTWPLGSLNSTPTPSFLLPTATVT
                     PTIQPTPTRKPTATPAPTSTTPQPNFATPEPPTPTATTLPLPPVSCSAPNVQLTAPTP
                     GQIVSGEFQMFGTATAPNFAFYKFTLQGRGTNNVELTAGEAVRDPKQAAVLGSLNPSI
                     YFDQPGVYLLGLVVVDNTGSEYPHCVIPIIIQPPPP"
     gene            2207..2746
                     /locus_tag="VFF70_12610"
     CDS             2207..2746
                     /locus_tag="VFF70_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45578.1"
                     /translation="MNKVYVRTARADDLKQCLAIDPSFNTDHVWQMESRSEEGQISVS
                     FRTTRLPRLMQVAYPRDAKSLAASWPACATMLIADSNRQLVGYVALSKRVPQSAVWLD
                     DLVVDRTARRDGVGSALLEAAGRWGVTQKMKWLIIEVQTKNYPAICFCQKHGLTFCGF
                     SDRHFQNQDIALFFAQALH"
     gene            2786..>3657
                     /locus_tag="VFF70_12615"
     CDS             2786..>3657
                     /locus_tag="VFF70_12615"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HZY45579.1"
                     /translation="MIGSISLISILALINSILSTGTAVTAFSLLMYIVRYNWRAPVAR
                     AFSVLLACVMLVYVGDAILFHVSDQKPAELWLRMQWIGIALAPAAYLHFADAVLRTTN
                     SFTRLRRWGVYGAYALGAGFMLLALTTDVLVGDVVFVDSAPHLRPGPLFWLYIAYFAL
                     AVIIGFIAILQARSRCLTPTSHRRMTYLLLSSTGPALAVFPYLLLSSNSDAAVPLISW
                     VALVAGNFMVAAMLIVMGYSVAYFGVLAPDRVVKYRLVRFLFRGPIMAAAVAAAVTLF
                     TKLEEFLGLPGETLT"
BASE COUNT          725 a          988 c         1014 g          930 t
ORIGIN      
        1 actccgtcct gcgatttgcg ccgctgattt ttgtggtggc attaatctat cccctcggcc
       61 gattgggctg ggactatctg caatggtcgg cggagcaata tttggtcacc tcacaccgtg
      121 tcatccagac tgagggcttg atcaataagc gaactaaaga ttcttcattg gaaaaggtga
      181 acgacattca gctaacacag tcgttcttcg gtcgattgtt gggctatggt gagttggaga
      241 tcgtcactgg cagcgaggaa ggcgtgaaca tattgcaccg gctcaataat ccggtgtctt
      301 ttaagaccgc gctgctcgat caaaaagtcg cgctcggtca gaatgctggc ggggatcaag
      361 tccgacccgg cggtgcggcc ggcacagccg gtggcgatga ctcgcttaag cgtttaggcg
      421 atttagatga tctgcgcaag cgcggcgcaa tcagcgatca agaatatcaa gaggctaagg
      481 ccaagattct cggcaagctt taatgacacg gcgtgcgctg tagtttaact gcggcgcaac
      541 ccttcaccgg attggttccg atgtccgaca ccattacccg cgaacagatc gatgaagcag
      601 ttgcgctgat ccgacaacac accgctcagc tgccagaagt tggcatcgtg ctcggttcgg
      661 gattaggtga attagccgct gcggtcgagc ggccggatat tattccattt gccgatattc
      721 cgcattttcc aatctcgtcc gttctcggcc atgccggtcg aatgttaatc gggcagctgg
      781 aaggccgatc ggttttggtg atgcagggcc gcgcgcattt ttacgaaggc tattcgatgg
      841 ggcaggtaac tttcccgatc cggctgatgc aattcctcgg catcaagact gtgattctca
      901 cgaatgccgc gggcggctta aacccgtctt tcaattcggg cgatgtcatg ttgattaccg
      961 atcatattaa cttgatcggc atggccggca acaatccact gcgcgggccg aacgatgaat
     1021 cattcggcac acgtttcccc gatatgtctg aaccgtacga taagcagctc ggatcgatcg
     1081 cactgcagac agccgcacaa aataaaatcg atctaaggca gggtgtgtat atgtgtctgg
     1141 ccggtcccag ttttgaatcg ccggctgata ttcatttctt gcgcatgatc ggtgcggacg
     1201 cggttggtat gtcgactgtg ccggaggcga tcgtcgcgcg gcacggcggt ctgcgcgtgc
     1261 tgggcttatc cggtatcagc aattcgctgc caccggctgg cgtcaaacaa gaaacaactc
     1321 acgccgaagt ccttgctgcg ggccgcacgc ttgtaccgaa aatgatcacg atcattcgcg
     1381 gcgtgctgcg ctcggtttag ttgctatggt cttcttcgtt cagctgctcg ctaattacgc
     1441 ggggttgatt tacatcggct gcgccattgg cgcgctcatt tatctccggg atattctggc
     1501 cgcgcgtagc gaaatacaaa cctcactcta ttcactcgaa cgggaagggg caagtagccg
     1561 cgtcatgcgc ggatcgatca tggtcgtgct gtttatcggt gtggctattg tcgcttacat
     1621 cttagcgaac gtggttgcgc cgacatggcc tttgggttct ttgaatagca cgccgacgcc
     1681 ttcattcctt ttgccaactg ccacagtaac tccgacgatt cagccgacgc cgacccgcaa
     1741 gcccactgcg acgccagcgc caactagcac gacgccacag cccaattttg caaccccaga
     1801 accgccgaca cccacggcca cgacattacc tttgccgccc gtgtcttgtt cagcgccaaa
     1861 cgtgcaattg actgcgccaa ctccgggcca aattgtatcg ggcgaatttc aaatgtttgg
     1921 caccgctact gcgcctaatt ttgcttttta taaattcacg ctgcaaggcc ggggcacgaa
     1981 caatgtcgaa ttgacggccg gtgaggccgt gcgtgatcct aaacaggcgg ctgtgttagg
     2041 ttcgctcaac ccgtcgatct attttgatca gccgggtgtc tacctattag gtctggttgt
     2101 ggtcgacaat acgggcagcg aatatcctca ctgtgtgatt ccgattatca ttcaacctcc
     2161 tccgccgtaa acctctgcaa ctctcgatcg tggtagaatg atggcgatga ataaagtcta
     2221 tgttcgaacg gcgcgcgccg atgatcttaa gcagtgttta gcgatcgatc cgagtttcaa
     2281 taccgatcac gtctggcaga tggagagccg atcggaagaa ggacagatct cggtttcgtt
     2341 tcgaacgacc cgtttacccc gattgatgca ggtggcttat ccgcgtgatg ccaaatcttt
     2401 agcggccagc tggccggcgt gcgccacgat gttgatcgcc gacagcaatc gtcaactggt
     2461 tggttatgtg gcgctctcta agcgtgtgcc gcaatcggcg gtctggttgg acgatctggt
     2521 agtcgatcgg actgcgcgtc gagatggagt gggcagcgcc ttactcgaag cggcgggccg
     2581 gtggggcgtg acgcaaaaaa tgaaatggct gattatagaa gttcagacta aaaactatcc
     2641 ggcgatttgt ttctgccaga aacacggctt gacgttttgc ggctttagcg atcgacattt
     2701 tcaaaatcaa gatattgcgc tgttctttgc tcaagcattg cattaaagac tggaatcgct
     2761 agatgtttta tgtgtattga tgtttatgat tggctctatt agtctgatat ccatcctcgc
     2821 gctaatcaac tccatcctct ccaccggaac tgcggtcact gcattttcgc tgctcatgta
     2881 catcgtgcgt tacaactggc gcgcaccggt ggctcgagcg ttcagtgtgc tgctggcctg
     2941 cgtcatgttg gtctacgtcg gcgatgcgat tctgtttcac gttagcgatc agaagccggc
     3001 ggaattgtgg ctgcgcatgc aatggatcgg cattgcgctg gcaccggctg cttatctgca
     3061 cttcgccgac gcagtcttgc ggacaacgaa ttcgtttacg cgactgcggc gctggggcgt
     3121 gtatggtgct tacgcgctag gcgcaggctt tatgctgctc gccctcacga ccgatgtcct
     3181 tgtgggcgat gttgtcttcg tcgactcagc cccgcacctg cgtcccggcc cgttgttctg
     3241 gctgtatatc gcttacttcg ccttagcggt catcatcggc ttcattgcga tcttgcaggc
     3301 gcgcagccgc tgcttgacac cgacttcgca tcgtcgcatg acgtatttgc tgctgtcatc
     3361 gactggccct gcactggcgg tctttcctta tttgctgtta tccagcaaca gtgatgcggc
     3421 cgttccattg atttcatggg tcgccttagt cgccggcaac ttcatggtcg cggcaatgtt
     3481 gatcgtcatg ggttacagtg tcgcttactt cggcgtgctc gcgcccgatc gcgtagtgaa
     3541 ataccgcttg gtgcggttcc tgtttcgcgg cccgatcatg gccgcggccg tggccgctgc
     3601 cgtcacgctg tttacgaagc tggaagaatt tcttggcctg cccggcgaaa cgctgac
//
LOCUS       DASRTJ010000618         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_169463,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000618 DASRTJ010000000
VERSION     DASRTJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169463"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFF70_12620"
     CDS             <1..421
                     /locus_tag="VFF70_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY45580.1"
                     /translation="DRLDVLITNAGLYLMERLVSEDGHEMMLAVNHLAHFLLTNLLLD
                     RLKASAPSRIINVSSGMHHGGAIDFDDLHSQKSFHGSRVYSNTKLANILFTHELAERL
                     KGTGVTVNSLHPGGVSTKLFRGTNGISVEEVAATPVY"
     gene            853..2049
                     /locus_tag="VFF70_12625"
     CDS             853..2049
                     /locus_tag="VFF70_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45581.1"
                     /translation="MRNRWWIVLILGFIGFVTSFGAHIVAVNLPIYAEQVGVGVAIIG
                     ILIAAYDFAEIVAKPIFGALADRRGMQQTMLAGIAVFTLASLAYLVVDPKLLIVVRFV
                     QGVGAAALSAVSLALVGVYFTEQRGRAYGIYNAIKGAGYVVSPIIGSAIVMHSNFAAI
                     FLAAAAIGVMAFVFALLLPEPRATQGASVHTQVFDDDDDLSLKTFLAVFKQPNLLPWY
                     LVIVVNMFFVGILFGFLPVRIHDLHYDPLTTGLILTAATSSYLLIQPIAGALADRINV
                     ATTVRIGLLLSGIAIILVPFVEGSPLVLVSILAGIGVGTVWTNTDTLVSTLAKSGQLG
                     ATMGAAGSFKEFGDMLGPLLIGFLSQAFGLTAGFVVCGGLGLMSIMLIRTATKEMQPK
                     DERNTA"
     gene            2046..3119
                     /locus_tag="VFF70_12630"
     CDS             2046..3119
                     /locus_tag="VFF70_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZY45582.1"
                     /translation="MTRKRLRDLGITIGTLPTGPNNAITDVDGVRVGHTTLITDEPRI
                     ARTGVTMVVPRDGSIWHDNAPAAYHILNGAGEMSGTHWLNEVGVLSSPIGLTNTHQVG
                     LLRDSMESYAYEKGFTSSFLLPVATETYDGWLNDIYAAHVTKADIYHAIDSATSGPVA
                     EGNVGGGTGMICHEFKGGIGTSSRVIEVKSGRFTIGVLVQANYGDRKHLRIDGVPIGL
                     ELDYDHTPRAKIEYHPEGSIIVIIATDAPLTPGQCKRLAQRATIGLGRVGGMGHTSSG
                     DIFLAFATGNHIPDRSDNLIDLKMLPHLHLDPFFDGVVEATEEAIINALCMAETMVGF
                     KGRTVYELPLDEVQRVMKKYNRL"
     gene            3233..3532
                     /locus_tag="VFF70_12635"
     CDS             3233..3532
                     /locus_tag="VFF70_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45583.1"
                     /translation="MIHIIWEFNVHSDRVEKFLQHYSSEGTWAQLFQKSAAFKETLLE
                     RDVDSPLRFITIDKWADRSGFEDFKRTFQAEYAALDRRCESLTESDHFLGMFESL"
     gene            3633..4265
                     /locus_tag="VFF70_12640"
     CDS             3633..4265
                     /locus_tag="VFF70_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZY45584.1"
                     /translation="MATILQDARHDILHIIREKGQSDITELSRALGLSPVTVHYHVNV
                     LQREGLLEITPVRNGVGRPRNVFTLRPEALNQFPQSYHRLSDRLLDTLKTRLSETELL
                     EVFEKMADEIAAEHATLLQGKSLEQKIETLIDLLGEEGFMSKLEKTGAHDFVLTQISC
                     PYQYVVARHPEVCELDLRLINVALGTPVKREACLAHGDTVCTFHISAQPD"
     gene            4274..4603
                     /locus_tag="VFF70_12645"
     CDS             4274..4603
                     /locus_tag="VFF70_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZY45585.1"
                     /translation="MSDTPEAEVIEYSPEETALRDALRAVLDPELGMSVVELGLIRKA
                     NFMPDLTELTVIMTTPFCPYGPALIEQIRRTAEQHLKTPVKLTLGMEMWEPSMMEGGP
                     PAEWGLY"
     gene            4728..>5376
                     /locus_tag="VFF70_12650"
     CDS             4728..>5376
                     /locus_tag="VFF70_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZY45586.1"
                     /translation="MAEYKEPITPKRVMTIHAHPDDQEVSVGGTIAKWAKGGSEIICV
                     VVTNGEAGAGDEGNRDPSKNDAFKPHLIKMREGEQFAANKLLGVRETIFMGYPDGELQ
                     PTLELRKKLTRLIRKYKPDVVVTSDPTVRFYGSSYMNHPDHRVVADVAIDAIFPSAGT
                     RLIFTDLLKEGLEPHDVKKVFMHGTDKPDTWVDISETIDIKAQALKLHTSQLFTDE"
BASE COUNT         1271 a         1378 c         1489 g         1238 t
ORIGIN      
        1 cgatcgatta gacgtgttga tcaccaacgc gggactgtat ctcatggagc gcctggttag
       61 tgaggacggg catgaaatga tgctggccgt gaatcacctg gcgcatttcc tcttgaccaa
      121 cctgctgctc gatcgattga aggccagcgc gccttcacgc atcatcaacg tcagttccgg
      181 tatgcaccac ggcggtgcga tcgactttga cgatctgcat tcgcaaaaaa gttttcatgg
      241 ctcgcgcgtg tattcaaaca cgaagttggc aaacatttta ttcacgcatg aattggccga
      301 gcgtttgaag gggacaggcg tcacagtgaa ttcgctgcat ccgggcggag tgagcaccaa
      361 actgtttcgt ggcacgaacg gcatcagcgt cgaagaagtc gcggctacac cggtttatta
      421 ggccacttcg cccgaagttg aaaaagtaac ggggaaatac ttcgaccgga aacggatcgt
      481 cgaaacttcg gccgcgtcgc gcgatccagt cctgcagcaa gtactacggg aagtgagtac
      541 acgcctgacg ggactgtctt aaaccgatta gttaatcaga gccggacacg gcttatcatc
      601 gaagtggcag ccacgcttgt gtgctaactg acgtgccgtc acagcgacat cctcgtcggt
      661 ggagtgtaac gcactctgcc agattgattt gcccgcgagc gacatcagat tgaacaccac
      721 ttgcgcgctg cgcgctttca cttcacaggt tggccagggc tgattcgata aaaagaaaaa
      781 cggtcgaatt acagccgttg agatttacca ttatactgat cagagaaatt ttcgttcaga
      841 cgaggtttca gtatgcgcaa tcgatggtgg atcgttttga ttctcggctt catcggcttt
      901 gtgacctcgt ttggcgcgca tatcgtcgcg gtcaacctgc caatttatgc cgagcaagtt
      961 ggggtgggcg tggcgatcat cggcatcctg atcgcggctt acgacttcgc cgagattgtt
     1021 gccaagccta tttttggcgc gttagccgat cggcgcggca tgcagcagac tatgctagcc
     1081 ggcattgcgg tgttcacgct tgcttcactg gcctatttgg tggtcgatcc gaaactttta
     1141 attgtcgtgc gcttcgtgca gggtgtcggc gcggcggcgt tgtcggccgt gtcactcgct
     1201 ttggttggcg tttattttac cgagcaacgt ggccgtgcgt atggcattta caacgcgatc
     1261 aaaggcgcag gctatgtggt gagtccgatc attggcagcg cgatcgtcat gcacagcaat
     1321 ttcgctgcga tctttttggc cgccgccgcg atcggcgtca tggccttcgt gttcgcgctg
     1381 ctgctgccgg agccgcgcgc cacccaaggc gctagtgtac acacacaagt cttcgacgat
     1441 gatgacgatc tttctttaaa aactttcctg gcggtattca agcagccaaa tttgttgccg
     1501 tggtacttgg tcatcgtcgt caacatgttt tttgtcggca tcctgttcgg ttttctaccg
     1561 gtgcgcattc atgatctgca ctacgatcct ttgaccaccg gattgatctt gaccgcggcg
     1621 acctccagtt acttattgat tcagcccatc gccggcgcgt tagccgatcg gatcaatgtg
     1681 gcgacgactg ttcgcatcgg actgctgctt tcgggcatcg cgatcattct ggtgcccttc
     1741 gtcgaaggca gcccgttggt gctggtgtcg atcctggctg gcatcggtgt cggcaccgtg
     1801 tggaccaaca cggatacact ggtcagcaca ctcgccaaat ccggtcaact cggagcgacg
     1861 atgggcgcag ccggatcatt caaagaattc ggcgacatgc tcggtccgct gctaatcggt
     1921 ttcctgtcgc aggccttcgg acttaccgcc ggctttgtgg tctgtggagg gctgggtctc
     1981 atgtcgataa tgttgatcag aaccgcgaca aaggaaatgc aacccaagga tgagaggaac
     2041 acagcatgac gcgaaaacga ttaagagatt taggcatcac gattggaact ctgcccactg
     2101 gtccgaacaa tgccatcacc gatgtggacg gcgtgcgggt cggtcacacc actttgatca
     2161 ccgatgagcc gcgcatcgcg cgcacgggcg tgacgatggt cgtaccacgc gacggatcga
     2221 tttggcacga caacgcgccc gcagcttatc atatcctgaa cggagcaggt gaaatgagtg
     2281 gcacgcattg gctcaacgaa gtcggcgtgt tatcctcgcc gatcggcttg acgaacactc
     2341 accaagttgg gctactgcgc gattcgatgg aaagttatgc gtatgaaaaa ggcttcacgt
     2401 catcatttct actaccggtg gcaaccgaga cttacgatgg ctggctgaat gatatttacg
     2461 cagcgcatgt taccaaagct gacatctatc acgcgatcga ttcagccaca agcgggccag
     2521 tggccgaagg caatgtcggc ggcggcacag gcatgatctg ccacgaattc aaaggcggta
     2581 tcgggacttc atcgcgtgtg atcgaagtga agagtggccg atttacgatc ggggtgttag
     2641 tgcaggccaa ttacggcgat cgaaaacacc tgcggatcga cggtgtaccg atcggacttg
     2701 agctggacta cgatcacaca ccacgcgcaa aaatcgagta tcaccctgaa ggatcgatca
     2761 tcgtgattat tgcgaccgat gcacccctga cacctggtca atgcaaacgg ctggcacagc
     2821 gcgcgacgat cggcttgggg cgagtcggcg gcatgggaca taccagcagc ggcgacatct
     2881 ttctggcgtt cgcgactggc aatcacattc ctgatcgctc ggacaacctg atcgatctca
     2941 agatgctgcc gcatctgcat ctggatccat tctttgatgg agtggtggaa gcgaccgaag
     3001 aagcgatcat caatgcgttg tgcatggctg aaacgatggt cggtttcaag gggcggacgg
     3061 tatatgagct gccgcttgac gaagtgcagc gcgtgatgaa gaaatacaat cgattatgat
     3121 gtaggggcga gacattccca atgaagccag attaaacgaa atccaaatgc aatcgaggta
     3181 ttgtgttaaa tcgatctgcg tggaaagcct cacccccaac ggcggagaca aaatgattca
     3241 catcatctgg gaatttaatg tgcattcgga tcgagttgaa aaattcttgc aacattattc
     3301 cagcgaggga acttgggccc agttatttca aaagagcgca gctttcaaag aaacgctgct
     3361 tgaacgagat gtcgattcgc cgctgaggtt tatcacgatc gataagtggg cggatcgatc
     3421 gggttttgaa gatttcaagc gaacttttca agcagaatat gcagcactcg atcggcgctg
     3481 cgagtcgctg acggagtctg atcatttcct cggcatgttt gagagccttt aagtgccgat
     3541 caaacaatcg ggtattgaac attctggcaa tttcctcaat cgatataaga aatattgaca
     3601 aacagtttgc agctgatata atcacttcac tcatggccac aatcttacaa gacgcacgcc
     3661 acgacattct gcacatcatt cgtgaaaaag gccagtccga catcaccgaa ctttcacgcg
     3721 cattgggcct gtcgccggtc acggtgcatt atcacgtgaa tgttttgcag cgcgaaggtt
     3781 tattggagat cacgccggtc cgcaacggcg tcggccggcc gcgcaatgta ttcacgctgc
     3841 gcccggaagc gctcaatcaa tttccgcaaa gttatcaccg cttgagtgat cggctgctcg
     3901 atacgttgaa gacccgctta agcgaaaccg aactacttga agttttcgaa aagatggcgg
     3961 atgaaatcgc cgccgagcat gccacgttac tgcaaggcaa atcgctggag caaaaaatcg
     4021 agaccctgat cgatctgctg ggcgaagaag gcttcatgtc gaagctggaa aaaaccggcg
     4081 cacatgattt tgtgttgacg cagattagtt gtccatatca atatgtcgtg gcacgccatc
     4141 ccgaagtatg cgaactcgat ttgcgcctga tcaatgttgc actcggtacg cccgtcaagc
     4201 gcgaggcttg tttggcgcac ggcgatactg tgtgcacctt ccacatcagc gcacaacccg
     4261 attaggagtc aatatgtcag acacgcctga ggctgaagtc atagaatatt ctccggagga
     4321 aacagcgctg cgcgatgcac tgcgcgctgt tttggatccc gaactgggta tgagcgtggt
     4381 tgagttggga ctgattcgca aagcaaattt catgcccgac ctgaccgagc tcaccgtgat
     4441 catgaccacg ccgttttgtc cctacggccc ggcgctgatc gaacagatcc gccgcacggc
     4501 cgagcaacac ctgaagacgc cggtgaaact cacattgggc atggagatgt gggaaccatc
     4561 gatgatggaa ggcggaccac ctgccgagtg ggggttgtac tgatcgattt gtagggacca
     4621 gctttgtgct cgtcactacg catagaatta atgtaacaaa tcgatcgggg cacactgccc
     4681 gatcgatttt ttgttgaaca acctgcgatt cattcaagga gatgattgtg gccgaataca
     4741 aagagccgat tacacccaaa cgcgtgatga cgattcatgc ccatcccgac gatcaagaag
     4801 tctcagtcgg cggcacgatt gccaaatggg ccaagggggg cagcgaaatt atttgcgtgg
     4861 ttgtcaccaa tggcgaagcg ggcgcgggtg atgaaggcaa tcgcgatccg tcgaagaatg
     4921 acgccttcaa gccgcacttg atcaaaatgc gcgaaggcga gcagttcgcg gcgaacaaat
     4981 tgctaggtgt ccgcgaaacg atcttcatgg gttaccccga tggtgaattg cagccgactt
     5041 tggaactacg caagaagttg acgcgcttga ttcgcaaata caaacccgat gtggtggtga
     5101 ccagcgatcc gaccgtgcgc ttttatggca gcagctacat gaatcatccc gatcatcgcg
     5161 tcgtcgccga tgtcgccatc gatgcgatct ttccttcggc aggcacccgc ttgatcttca
     5221 cggatttatt gaaagagggc ttggagccgc acgacgtgaa gaaagttttc atgcacggca
     5281 cggacaagcc cgacacatgg gttgatattt cggagacgat cgacattaag gcacaagcat
     5341 tgaagttgca cactagtcaa ttgttcactg acgagg
//
LOCUS       DASRTJ010000619         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_284398,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000619 DASRTJ010000000
VERSION     DASRTJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284398"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VFF70_12655"
     CDS             complement(<1..1489)
                     /locus_tag="VFF70_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZY45587.1"
                     /translation="MPEAAIFCDHCGRQLTSQFGAPLASTVQPLLDAPTVNKVSAAPR
                     PKPAVPPSEAEAQLQQYIPKELMKKLDAARTRSEMVGERRVVTMLFCDVKGSTAAAEQ
                     LDPEDWSEIINGAFEHMIKPVYKYEGTLARLMGDAILAFFGAPIGHEDDPQRAVLAGL
                     DIVTAIQPYCERMKQKWGIDFNVRVGINTGLVVVGAVGSDLRMEYSALGDAVNLAARM
                     EQTAAPGTVQIAYDTYKIIKGTFEVEDLGGIEVKGKTEPVLAYRVLARKAAGVRIRGI
                     EGLSVEMVGRTTELSTLRSVIVNLKQGVGRIVCVLGEAGLGKSRLVSETAKVFHAELG
                     SQGDWHETISLSYETNQAYGLFQRLILNMNGIAYNDAPSIVREKLIGLTDSLPVAKRP
                     RALQAFETLFGLASDAERDTPPLEGEAFKLELFEAIASWWRTRCANHPTVLVFDDMHW
                     SDAASIELLNRLLPLTSEIPLVLLCAMRAERSAPAWQIKTVAADEF"
BASE COUNT          335 a          483 c          441 g          336 t
ORIGIN      
        1 ggaactcgtc ggccgcgacg gtcttgatct gccaggcagg cgcgcttcgc tcggcacgca
       61 tggcgcacag caacaccagc ggaatttccg aagtgagcgg caagagccgg ttaagcaatt
      121 caattgaagc ggcatcgctc cagtgcatgt cgtcgaacac cagcaccgtg gggtgatttg
      181 cacaacgcgt gcgccaccaa ctggcgatcg cttcaaacag ttcaagtttg aaagcctcgc
      241 cttccaacgg cggagtgtcg cgctccgcgt cgctggccag tccgaataaa gtttcgaagg
      301 cttgcagcgc gcgcgggcgc ttcgcaacgg gcaggctatc ggtcaacccg atcaatttct
      361 cacgcacgat cgagggcgca tcgttatagg cgatgccatt catgttcaag atcaagcgtt
      421 gaaacaaacc gtacgcttga ttggtttcgt aagacaaact gatggtctca tgccagtcgc
      481 cctgggagcc cagctcagcg tggaaaacct tagcggtttc actcaccaag cggctcttgc
      541 ctaagccggc ttcgcccaac acgcagacga tccgccccac accttgtttc agattgacga
      601 tcacactgcg cagagtggac agctcagtcg tgcgtccgac catttcaacc gatagaccct
      661 cgatgccgcg tatgcgtacg ccagccgctt tgcgcgccag cacccgataa gcgagtacag
      721 gctcggtctt gcctttgact tcgatgccgc cgaggtcttc aacctcgaac gtgcccttga
      781 tgattttgta agtatcgtaa gcgatttgca ccgtgcccgg cgcggcggtc tgctccatgc
      841 gggcggcgag attaaccgca tcccccaagg cagaatattc catccgcaag tcggatccga
      901 ccgcgccgac gacgaccaat ccggtgttga tgcccacccg cacgttgaag tcaatgcccc
      961 acttctgctt catccgctcg caataaggct gaatggcggt cacgatgtcc agtccggcca
     1021 gcactgcccg ctgcggatcg tcttcatgcc cgatgggcgc gccaaagaaa gccagaatcg
     1081 catcgcccat caagcgggcc agcgtgcctt catatttata aacaggtttg atcatgtgct
     1141 cgaacgcgcc gttgatgatc tcgctccagt cttccggatc gagttgttcg gcggcggcgg
     1201 tcgaaccttt cacgtcgcaa aacagcatcg tcacgacgcg ccgttcgccg accatctcac
     1261 ttctggtgcg ggcggcatcc aattttttca tcagttcttt ggggatgtat tgttgaagtt
     1321 gagcttcagc ttcagatggc ggaaccgccg gcttcgggcg cggcgcggcc gaaactttgt
     1381 taacggtcgg cgcgtccagc agcggttgaa ccgtgctagc aagtggggct ccgaattggc
     1441 tggtcagttg gcgtccgcag tgatcgcaga agatcgcggc ctcgggcaaa gcagcatcac
     1501 agaacgggca attcggcaga agccagcaac cgcagcgctt gcaaaatctc gattcccgac
     1561 gattttcatt ttggcaattg ggacaaatca tattc
//
LOCUS       DASRTJ010000620         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_207295,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000620 DASRTJ010000000
VERSION     DASRTJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207295"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1470)
                     /locus_tag="VFF70_12660"
     CDS             complement(295..1470)
                     /locus_tag="VFF70_12660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZY45588.1"
                     /translation="MKPEYDVVVIGAGPAGSIAARTTAQSGLATLLLEKRQEIGSPVR
                     CAEAVGKEAIEKFIPLDLKWIAAEIETFSLTNPQGDCVVLPPLEHTLVLERKIFDREL
                     AHSAAKAGAEVMVKARVTDLIKSARGIEGVRINVQGKRHDVRAKIVIGADGTESQSPR
                     WAGLKSIPQLKDYYTAAQYLMTDIDIDPRICQYHLGWSLAPGGYVWVFPKGDRQANVG
                     LVMWVDPKEKHTAIDYLNEFVAARFPHSSILSQVVGGIPITNVLPQMVTDGYMAVGDA
                     AHQSDPFTAGGITNGMHGGLFAAEVAIEAIKRGDTSGKFLKKYEDRWEAEFGKLYRRL
                     YRIRHALLKVPEEKISKMIHDASKLNARQMSLKDIFMIVMKAHPQMLLEVAPYFLGQ"
     gene            complement(1467..1679)
                     /locus_tag="VFF70_12665"
     CDS             complement(1467..1679)
                     /locus_tag="VFF70_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZY45589.1"
                     /translation="MSTQTLEVVLPIVLVDYDHCSYCGACVAVCPPDCITLHDATLVI
                     DQTACTSCQRCIPACPTGALTIETVA"
     gene            1836..2141
                     /locus_tag="VFF70_12670"
     CDS             1836..2141
                     /locus_tag="VFF70_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45590.1"
                     /translation="MAKAKNKSVFMAGKAEWLSKNARQLAPEQRALLLAVARLDVESG
                     HKLTAEEQAALDELSANSDGFDPKEIQDAVHHMVEAKTKRKTIDWPSGLYRLLRKKK"
     gene            2298..2801
                     /locus_tag="VFF70_12675"
     CDS             2298..2801
                     /locus_tag="VFF70_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45591.1"
                     /translation="MQQPMTQQPMMQQPIKKQRPIGISVLAILAILLGLLLLVGGVIA
                     MAGGNFLGALMSSAVTDAGVTGADANVVGAAVGAMGTILGIGVVIAGLLWLLVGIGAW
                     NLKGWARWLGIIASLLSFLGVGLPVIQAMLGGLQVSGTQIAEAVIPVLIFIYLLTPGV
                     AKAFSKN"
BASE COUNT          664 a          765 c          789 g          684 t
ORIGIN      
        1 tcagcgcatc gatcgagatt atttcatcga caagctgcaa gccgacttcg gcgatcgtgc
       61 cgctgatcaa gtggacactg ccgtcgattg gggtcgttat gcggagttat tcgcgtacga
      121 tgacaatacg gatgagttgt atcttgaagg gaatggaacg agtaatgcgt gaggaaattt
      181 gtttgcgtag gggcgaggca ttcccagcag attattattc gactgcatgg caattgcagt
      241 tgcgcgccaa tcgaaacaga tcgattggga atgcctcgcc ccaacaatca tcgtttattg
      301 ccccaagaaa tacggcgcga cttcgagcag catctgcgga tgtgccttca taacgatcat
      361 gaaaatatct ttgagcgaca tctgccgcgc gttgagtttc gacgcgtcgt gaatcatctt
      421 gctgattttt tcttccggta ctttcagcag tgcatgccga attcgatata agcggcggta
      481 tagcttaccg aactcggcct cccagcgatc ttcgtatttc ttcaagaact tccctgaagt
      541 gtcaccgcgt ttgattgctt cgatcgcaac ctcggccgcg aacaagccgc cgtgcatccc
      601 gttggtaatg ccacccgctg tgaacggatc ggattgatgg gcggcgtcgc cgacggccat
      661 gtaaccatcc gtcaccatct gcggtagcac gtttgtaatc ggaataccac ccacgacttg
      721 cgacaagatc gatgagtgcg gaaaccgggc agcgacaaat tcattcagat aatcgatcgc
      781 ggtgtgcttc tctttcggat cgacccacat caccaagcca acgtttgcct gtcgatcgcc
      841 tttcggaaag acccacacat acccgccggg cgcgagcgac cagccaaggt gatattggca
      901 gatacgcgga tcgatgtcga tgtcggtcat caaatactgc gcggctgtgt aataatcttt
      961 cagctgcgga atcgatttga gtccagccca gcgcggactt tgcgattcag tcccatccgc
     1021 accaatgacg atcttggcgc gcacgtcgtg tcgcttgccc tgcacattga tccgcacgcc
     1081 ttcgatgcct cgagcgcttt tgatcagatc ggtcacgcgc gctttgacca tcacttcggc
     1141 gccggccttc gccgctgaat gtgccaactc tcgatcgaag atttttcttt ccaagaccaa
     1201 ggtgtgttca agcggcggca gcacgacgca atcgccctgc ggattggtga ggctgaacgt
     1261 ttcgatctcg gccgcgatcc atttgagatc aagcggaatg aatttctcga tcgcttcttt
     1321 gccgacggct tcagcgcagc gaacaggcga tccgatctcc tgccgcttct ccaggagcag
     1381 tgtcgctaaa cctgactgcg cggtggtccg cgccgcgatc gatccggcag ggccggcgcc
     1441 gatgacgacg acatcatact cgggcttcat gctacagtct cgatcgtgag cgcaccagtc
     1501 gggcaagccg gaatacagcg ctgacagctg gtgcaagcag tctgatcgat cactaaggtc
     1561 gcgtcgtgca gcgtaataca atcgggcgga caaacagcaa cacaggcgcc acagtaggaa
     1621 cagtgatcgt aatcgaccaa tacaatgggg agaacaactt cgagagtctg agtactcata
     1681 gcgcctccag gctaggagag aggtgtagtt gtacggccaa gacacaacca ttatatccgc
     1741 cctgctcgct atccgtcaac cttttgactc gcggctggca tgcgagtata atccagtggg
     1801 tgatcctgct gtagcgcagc tgagaggata aggccgtggc gaaagcgaag aacaaatcag
     1861 tctttatggc gggcaaggcc gagtggctat cgaagaatgc ccgccaactc gcgcctgagc
     1921 agcgcgcgtt attgctggcc gtggcccgcc tcgatgttga gtccggtcat aaacttactg
     1981 cggaagaaca ggccgcgctg gacgagttgt cggcgaacag cgatggcttt gatccgaaag
     2041 agattcaaga cgccgtccac catatggttg aagccaagac caaacgcaaa acgatcgatt
     2101 ggccgagcgg tttgtaccgg ctccttcgca agaagaagtg atcaatcagc tctacccaca
     2161 agagatcagc cagtctgatc tttttgtttt tcgcgcttcg acgcaaaatt tgactaatat
     2221 tgattcatcg ctacaatgtg ccttacagac ccggctcacg gcgggctgaa ttcaatcact
     2281 cttagggagg agttacaatg cagcaaccaa tgacgcagca accgatgatg cagcaaccaa
     2341 taaagaagca gcgcccaatc gggatatcgg ttttagcgat actagctatt ctcttgggac
     2401 tgttgctctt agtcggtgga gttatcgcaa tggcaggcgg taacttcctt ggggctttaa
     2461 tgtcaagcgc agttactgat gccggtgtga ctggcgccga tgcgaacgtt gtcggagcgg
     2521 ctgtgggggc catgggaact atcttaggca ttggagttgt catcgccggt ttactctggt
     2581 tactggtcgg cattggtgca tggaacttga agggctgggc tcgatggctc ggcatcattg
     2641 catcactgct gtcctttcta ggtgtcggac tcccagttat acaggcaatg ctaggtggtc
     2701 tacaggtgag cggcacgcag attgcggaag cggtaattcc tgtgttgatc tttatttatc
     2761 tactcacacc cggtgtcgct aaagcatttt cgaaaaacta atcatcatcg gggtggccat
     2821 taacagcggc ggaaattatt tccgccgctg ttttatttga gcaggctatc attttcaacc
     2881 gaacaataaa gcgcggcttg ct
//
LOCUS       DASRTJ010000621         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_169938,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000621 DASRTJ010000000
VERSION     DASRTJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169938"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..975)
                     /locus_tag="VFF70_12680"
     CDS             complement(133..975)
                     /locus_tag="VFF70_12680"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZY45592.1"
                     /translation="MSEYVHPEVLVETDWVAQHINDPKVRIVESDEDVLLYDIGHVPG
                     SVKIDWHTDLNDSLIRDYLNADRFATLLSAKGIANDTTVVFYGDKNNWWACYALWVFK
                     LFGHTDGRIMNGGRKKWIDEKRELSKTVPGYPKATYKAPARDDKTIRAFRNDVQAHLA
                     KKLPLVDVRSPGEYSGELLHMADYPQEGAMRGGHIPGAKSIPWAKTVQEDGTFKSADD
                     LKQLYQPQGITADKDIIAYCRIGERSSHTWFVLTYLLGYPKVRNYDGSWTEWGNLVNV
                     PIEK"
     gene            complement(987..2423)
                     /gene="sufD"
                     /locus_tag="VFF70_12685"
     CDS             complement(987..2423)
                     /gene="sufD"
                     /locus_tag="VFF70_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZY45593.1"
                     /translation="MKTKTKSAAKRVAAQKTKTVSAKKPNVRRVTPKQSGVTLAPQLG
                     FTSDALDALIASRPFEPEWMRDRRRDSFHVLMDTPLPTRTDEAWRRTDFGSLRLKEIE
                     SVMSAIDGNTSIRSAPKSIKEVVNGKSTSASLVLAEGETVANNVAKDLAKRGVIFTDM
                     DTAIQKHADLIEPLFMTDIVKPNDGYFAALHGAFWQGGSFLYVPGGVEVPQPIRSGTW
                     LNQHRSSFTHTLIVMEPGSRAVFIDEYASATGDKQAFANGAVEIVVRDGAQLDYVNWQ
                     DWGRNIFNFTHERAKVMRDATLHWIAIGLGTKLTKSFIDAVLAGQGSTALMSGAYFVD
                     SNQHLDYDTEQNHMVPHTKSDLLYKGALKDKARSVWQGNIHVFPGAQRTDAYQANRNL
                     TLSHTARADSIPGLEIEADDVRCTHGAAISQVDREEVFYLMSRGLPQTVAEQMIVTGF
                     FQPVLDRIPMASVRTRLETSFALKMGLA"
     gene            complement(2505..>4841)
                     /gene="sufB"
                     /locus_tag="VFF70_12690"
     CDS             complement(2505..>4841)
                     /gene="sufB"
                     /locus_tag="VFF70_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZY45594.1"
                     /translation="YEHAIKRPMPTWGGDLSGLKLDDMFYYIRPSDKTQGNWDEVPDA
                     IKNTFDKLGIPEAERKFLAGVGAQYESEMVYHSIRENLQQQGVIFLSSDDGLKQYPDI
                     FKEHFGTVIPYTDNKFAAINTAVWSGGSFVYVPKGVNVEMPLQAYFRLNSENSGQFER
                     TMIIVDEGAQVHYVEGCFLAGARVRTRSGEKSIEQIAVGDEVLTHQGRYRRVYHTMNR
                     AYHNDIYRIRFFGDSDRELQVTKEHPLLIVRRERENERNRNFETEWASTSTLKVGDYL
                     AIPVPQPELEMALAGISVPLGRGRHAPIDRYVEFPQEPDFYRLLGYYFAEGHVDNEHY
                     LSLSFHVAESDLVSDAAKLIERYFGKAPIMNKPRQNGQTLVLSSTETARAFAREFGPT
                     VYDKHVPEWVLSAPTELLAEFVRGLWRGDGSYDSHKNMFRYNSISANLAYAFRDALLR
                     LGIAASVNRQERALPRQAMYVVVISSPFNPPFGEIVGVDAPAGSLSGSPFEMDDRFLY
                     VPVRSITSEETETEVYNFSVEEDESYVCEGVVSHNCTAPTYSSDSFHSGVIEIIVKKG
                     ARCRYTTIQNWSINVYNLVTQRAMVAEDGIMEWTDANLGSKLTMKYPSTYLMGKGARG
                     EILSMAFAGKGQHQDTGGKVVHVAPHTRSKITSKSISKDGGRASYRGLLKVYKGAEGS
                     SSHVQCDALLLDETSRTDTYPYIEVDDDRVTIGHEATVSKIGDEQLFYLMSRGLSEVD
                     ARAMVVSGFIEPLVKELPMEYAVEMNRLIHLQMEGSVG"
BASE COUNT         1116 a         1397 c         1163 g         1165 t
ORIGIN      
        1 tctcgtagtg atccaaaata aagtctaatt gctcttgtcg atccatcatc tcattctacc
       61 cttgaaatgc aaagagtgaa acgtcatacc ttttgcgttt cactcttcac ttgctcatca
      121 tgtttattcg gtttacttct cgatcggcac gttgaccagg ttgccccact ccgtccacga
      181 cccatcgtag ttgcgaactt tcggatagcc caacagatac gtcagcacga accatgtatg
      241 gctcgaccgt tcgccgatgc ggcagtaggc aatgatgtcc ttatcggccg tgatgccctg
      301 cggttgatac agctgcttca aatcatccgc tgatttgaac gtgccatctt cctgaacggt
      361 tttcgcccat ggaatgctct tggcgccggg aatatgaccg ccgcgcatcg cgccttcctg
      421 cggataatcg gccatgtgca gcaactcacc gctatactca cccggagatc gaacgtcgac
      481 cagcggcagc ttctttgcca aatgtgcttg cacatcattg cggaaagcgc ggatcgtttt
      541 gtcatcacgc gccggcgctt tgtatgtcgc cttcggataa cctggcacgg tcttgctcag
      601 ttcgcgtttt tcgtcgatcc attttttgcg gccgccattc atgatgcgac catccgtgtg
      661 accgaacaat ttgaaaaccc atagggcata acacgcccac caattatttt tgtcaccgta
      721 aaagacaacg gtcgtgtcgt tggcgatacc cttggccgac aggagcgttg caaatcgatc
      781 ggcattcaga tagtcgcgaa tcagtgaatc attaagatca gtatgccaat cgatcttaac
      841 cgagcccggc acatggccaa tatcgtacaa cagcacatct tcatccgatt cgacaatgcg
      901 caccttggga tcatttatgt gctgcgcgac ccaatcggtt tctactaaca cttccggatg
      961 cacatactcg ctcatcacac tctcccttaa gccagaccca tcttcaacgc gaaagatgtt
     1021 tccaatcgcg tgcggacact agccattgga attcgatcga gcaccggttg aaaaaatcca
     1081 gtgacgatca tttgctcggc gaccgtttgc ggcaggccac ggctcatcaa ataaaaaact
     1141 tcttcgcgat cgacttggct gatcgctgcg ccgtgcgtgc agcgcacatc atcggcctcg
     1201 atctccaaac cgggtatgga atcggcgcga gccgtgtggc ttaacgtgag gttgcgattc
     1261 gcctgatacg catcggtgcg ctgcgcgccg gggaatacgt ggatgttgcc ctgccagacc
     1321 gatcgagctt tatcttttaa cgcgccctta tacaacagat cactcttagt atgcggcacc
     1381 atatgatttt gctcggtgtc gtaatccaaa tgctgattgc tgtcgacgaa gtatgcgccg
     1441 gacatcaacg cggttgatcc ttggccggcc aacacggcat caatgaacga cttggtgagt
     1501 ttcgtgccca acccgattgc gatccaatgt aaagtcgcat cgcgcatcac tttcgcgcgc
     1561 tcgtgcgtga agttaaagat gttgcgaccc caatcctgcc aattcacgta atcaagttgc
     1621 gcgccgtctc ggacgacgat ctcgaccgca ccattagcga aggcttgttt gtcacctgtc
     1681 gccgaagcgt attcatcgat gaagaccgcg cgcgagccgg gctccatcac gatcaacgta
     1741 tgtgtgaatg aagatcgatg ttggttcagc cacgtccccg atcgaatagg ctgcggcact
     1801 tctacaccgc ccggcacgta caagaacgat ccgccttgcc agaacgcgcc atgcaatgcg
     1861 gcaaagtagc catcgttcgg cttgacaatg tcggtcataa acagcggctc aatcaagtcc
     1921 gcgtgctttt gaatggccgt atccatatcg gtgaaaatta caccgcgctt cgccaaatct
     1981 ttggcgacgt tattggcgac tgtttcaccc tccgctaaaa ccagcgaagc ggaagtgctt
     2041 ttgccattca ccacttcttt gattgatttc ggcgcagacc tgatcgaggt gttgccatca
     2101 atagcagaca tcaccgattc gatctctttc aatcgcaacg atccaaaatc ggtccgacgc
     2161 caggcttcgt ccgtgcgcgt tggcagtggc gtgtccatca acacgtggaa actatcgcgc
     2221 cgtcgatcgc gcatccattc tggctcgaac ggtcgcgacg cgatcaacgc atccaatgca
     2281 tccgatgtaa agcccagctg tggagcgagc gtcacacccg attgtttggg tgtcactcga
     2341 cggacgttcg gttttttagc ggaaactgtt tttgttttct gagctgcaac tcgtttagct
     2401 gcagattttg tcttcgtctt cacgggcaat tcagatgctc ctaaaaacaa ataacgagta
     2461 aggcctaaag gcctgattac tcgttactat ttactcaata cacatcaacc gaccgagcct
     2521 tccatctgca aatgaatcaa gcgattcatt tcgaccgcat actccatcgg caattccttg
     2581 accaacggtt cgatgaagcc cgacacgacc atcgcgcgcg catcgacctc gctcaagcca
     2641 cgactcatca agtagaaaag ctgctcatcg cctatcttgc tgaccgtcgc ctcgtgaccg
     2701 atcgtgacgc gatcatcgtc gacttcaata tacggatacg tgtcggtgcg gctagtctca
     2761 tcgagcagca gcgcgtcgca ttgaacatgc gacgatgaac cttccgcgcc tttatagact
     2821 ttcaatagac cgcgatacga cgcgcgtccg ccgtctttgc tgatcgactt cgaggtgatc
     2881 ttgctgcgcg tgtgcggtgc aacgtgaacg actttgccgc ccgtgtcttg atgctggccc
     2941 ttgcctgcaa acgccatcga cagaatttcg ccgcgcgcgc ctttgcccat caaatacgtc
     3001 gacggatact tcatcgtcaa cttgctgccg agattggcat cggtccactc catgatgccg
     3061 tcctcggcga ccatcgcccg ctgcgtgacc aagttgtaaa cattgatcga ccagttttgg
     3121 atcgtcgtat agcggcagcg cgcgcctttc ttgacgatga tctcgatcac gccggagtga
     3181 aaagaatcac tcgaataggt gggcgcggtg cagttgtgtg acacgactcc ctcacacaca
     3241 tacgattcgt cttcttcgac tgagaaattg tagacctcag tttccgtctc ttcactggtg
     3301 atactgcgca ccggaacata caaaaagcga tcatccatct caaaaggcga accgctcaaa
     3361 ctaccggcgg gcgcgtcaac gccgacgatc tcaccgaacg gaggattgaa cggcgaagaa
     3421 atgacaacca catacatcgc ttgacgcggc agtgcgcgtt cttgccgatt taccgacgcg
     3481 gcaataccca gacgcagcag cgcatcacga aacgcgtacg ccaaattcgc cgagatcgag
     3541 ttgtaacgga acatgttctt atgcgaatca taactcccat cgccgcgcca caaaccgcgc
     3601 acaaattctg ccagcaattc agttggcgcg ctcaacaccc attcgggcac atgtttatca
     3661 tagaccgttg gcccgaactc gcgagcaaaa gcacgggctg tttcagtcga cgacaacact
     3721 aaagtctgcc cattctggcg aggtttgttc ataatgggtg ctttgccaaa gtatcgctcg
     3781 atcaacttcg ctgcatcgct caccagatcg gattctgcca catggaaaga cagcgagaga
     3841 taatgttcat tatcgacatg gccttctgca aagtaatagc caagcagccg ataaaaatcg
     3901 ggctcctgcg gaaattccac gtaacgatcg atcggcgcat ggcgtccgcg gccaaggggt
     3961 acactgatgc cggcaagcgc catttccagc tcgggctgcg gaacggggat cgccagataa
     4021 tcccctacct tcagcgttga agtgcttgcc cactctgttt caaaattgcg gttgcgttca
     4081 ttctcgcgtt cacgccgcac gatcagtagt ggatgctcct tcgtcacttg taattcgcga
     4141 tcgctgtcac caaaaaagcg aatgcgataa atatcattgt gataagcccg attcatggtg
     4201 tgatatacgc gccgatagcg tccttgatgg gtcaagactt cgtcgccgac ggcgatctgc
     4261 tcgatcgatt tttcgcctga tcgagtccgc acccgcgccc cagccaagaa gcaaccttca
     4321 acgtaatgca cctgcgcgcc ttcatcgacg atgatcatcg tgcgctcgaa ctggccggag
     4381 ttctccgagt tcaaacggaa gtaggcttgc agcggcatct cgacattgac gcccttcggc
     4441 acatacacaa acgagccacc agaccagacg gcggtattga ttgccgcgaa tttattatcg
     4501 gtgtacggaa tcacggtgcc aaaatgttct ttgaaaatat cggggtattg tttcaagcca
     4561 tcatcgctgc tcaggaagat cacgccctgc tgctgaagat tttcccgaat cgagtgatac
     4621 accatctcgg attcatactg cgcgccgaca cctgccaaaa attttcgttc ggcttccggg
     4681 atgcctaact tgtcgaatgt atttttgatc gcatcgggca cttcgtccca attgccttgc
     4741 gtcttatcag atgggcggat gtaataaaac atgtcgtcca gcttcaagcc cgatagatcg
     4801 ccgccccacg ttggcattgg ccgcttaatg gcgtgctcgt a
//
LOCUS       DASRTJ010000622         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_359201,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000622 DASRTJ010000000
VERSION     DASRTJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359201"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1582)
                     /locus_tag="VFF70_12695"
     CDS             complement(182..1582)
                     /locus_tag="VFF70_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45595.1"
                     /translation="MTSAPYQPPPNGWRTFLIVWATQSISVFGSNLTFFALSIWLTLT
                     LYPRLDQKPELAVALSAISLSFALPRLFLIPIAGAYADRHDRKRIMMIGDAVAGLLSL
                     ILAVLVITNTLQLPGLISLLILHSIVGVFHGSAFDASYAMIVPEQKLPRANGLMQTMW
                     SLSGILSPSIAAAIIALPALLRQGGLTNGIAQLNDGSGLAMLIDAITFFIAAGSLIFL
                     VVPSPHRSDLQAPDGQRQKSLLADVKEGARYIWHRRPMLWLLGTFAVLNLLTAPIGVF
                     QPLILKFNLAPDWSSRGMTFEAALATLATISSIGGLIGGVAISTWGGLKKKRVFGVLV
                     PIVIVSVAMIGFGLSTVLYLTAVMAFISTAMIPIMNSHSQTIWQVQTPRELQGRVFAV
                     RRLIAQFTAPLGVGLIGLFGGRFDPGAVIAALGLILLIFSVIQLFNKNMLRVEDKEWL
                     DTMAAEKEARLVASNE"
     gene            complement(1630..2865)
                     /locus_tag="VFF70_12700"
     CDS             complement(1630..2865)
                     /locus_tag="VFF70_12700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45596.1"
                     /translation="MSDLKQLAQDFLTALATNTAERYAEIFTDDVGMLIGRWDGGEVH
                     RPQFRVVKRLIEEWSGWADPSVELLNVVGDDKLVAVEFRIQATENNRYVEHNRSAFLK
                     IKEGKIEQIRMYCSEPIPSARRKGWIAPLSIADDDLRRVFESMMNSSDPYEWIGTDES
                     GRWSLRGGMGGSGLPHPGSNGVGGVRWSAEEADRKIDETLEYHRQRNIGFQWWLAPDD
                     RPIDLGQRLEKHGLILAGHAATMARKGLDALDIPGNPDVTVELLEGYDEAAIDAIAHI
                     TMICFNWTQEQIDERRPGWIERMRDERFRQREGNFLARINGQAAGYGRVMLQSGVAYM
                     GGAGVLPEFRGQKVYSTLLRRRMEWAHEHNYHVAAINAEPLSRPIVERCGFKEYSRIY
                     IYAWMPVIDLDVIKSLVPQ"
     gene            complement(2966..3349)
                     /locus_tag="VFF70_12705"
     CDS             complement(2966..3349)
                     /locus_tag="VFF70_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45597.1"
                     /translation="MASAEERMQILKMIQEGKISAEEGSKLLQALSAGKADKRSPSST
                     TVGGDVRWFRVRVTDLASGKNKVNVNIPMGLVNVALKMGARFAPNIEGAKYNEMVQAI
                     KSGASGKIIDVTDDQDGERVEIFVE"
BASE COUNT          823 a          991 c          881 g          782 t
ORIGIN      
        1 cgaatagata tgacggaagc atcctgagcg gagcagcgaa ctcaaagagg tcgatgcgta
       61 gtcgaaggac gcgctcttgc accgacagaa tttctcgcat aaacgcgtcc ttcgactaca
      121 atgtgacttt tgcgaattca catctccact caggatgctt atatcatttg acgtggatcg
      181 tttattcgtt actcgctact aatctcgctt ctttttcagc cgccatcgta tccagccact
      241 ctttatcctc aacgcgcaac atattcttgt tgaacaattg aatgacgctg aagatcaaca
      301 gaatcaagcc taaggccgca atgaccgcac ccggatcgaa tcggccgccg aacaaaccga
      361 tcagaccgac gccgagaggt gccgtgaatt gtgcgatcaa tcgccgcacg gcaaacacac
      421 ggccttgcaa ttcgcgcggc gtttgaactt gccagatcgt ttgggaatgc gaattcatga
      481 tcgggatcat cgctgtcgag atgaaggcca tcaccgccgt gagataaagc acggtcgata
      541 aaccgaagcc gatcatcgcc acgctgacaa tcacgatcgg aacgaggacg ccaaacacgc
      601 gcttcttttt caagccaccc cacgtactga ttgccacgcc gccgatcaag ccaccgatgc
      661 tgctgatcgt tgctaaggtg gctaacgccg cttcaaacgt catgcccctg gatgaccaat
      721 ccggcgcgag gttgaatttc aagatcaatg gctggaacac gccgatcggg gcagtaagaa
      781 gattcaacac cgcgaacgtg cccaacagcc acagcatcgg ccgtcgatgc cagatgtagc
      841 gcgcgccttc tttcacatcg gccaataaac ttttctggcg ctggccgtcg ggcgcctgca
      901 aatccgatcg gtgcggcgat ggaaccacca agaagatcaa tgatccggca gcgatgaaga
      961 aggtgatcgc atcgatcagc atggccaagc ctgaaccatc gttcaattgc gcgatgccat
     1021 ttgtcaggcc gccctgtctc aacaatgcgg gcagggcgat gatcgctgcc gcgatcgatg
     1081 gcgagagaat gccggagagt gaccacatgg tttgcatcag gccgttggcg cgcggcaatt
     1141 tttgttcagg cacaatcatc gcgtacgagg catcgaaggc ggagccatga aacacgccga
     1201 cgatcgaatg caagatcaac aaactgatca accccggcag ttgcagcgtg ttcgtgatca
     1261 ccaagacggc caggatcaaa ctcaatagac cggcgactgc atcgccgatc atcataatgc
     1321 gtttgcgatc atgacgatca gcatacgcgc ccgcaatcgg gatgaggaac aagcggggca
     1381 atgcaaacga taaactgatc gccgataaag cgacagccaa ttcaggcttt tgatcgagtc
     1441 gcggatacaa cgtcagggtc agccagatac tcagtgcgaa gaaggtgaga ttgctgccga
     1501 aaactgagat cgattgcgtc gcccagacaa tgaggaacgt gcgccagccg ttaggcggtg
     1561 gttggtaagg tgccgaggtc atattatcaa tcagtatagg ccgctagggg caaactcgcc
     1621 cttataattc tattgtggca ccaaggattt gatcacatcc agatcgatca cgggcatcca
     1681 ggcataaatg tagatgcgcg aatattcttt gaagccgcaa cgttccacga tgggacgtga
     1741 gagcggctcg gcgttgatcg cagcgacatg atagttgtgt tcgtgtgccc attccattcg
     1801 tcggcgcagc aaggtggaat aaactttttg tccacgaaat tctggtagca cacccgcgcc
     1861 gcccatatac gccacaccgc tttgcaacat gacgcggcca taaccggccg cttggccgtt
     1921 gatgcgcgcc agaaaatttc cttcacgctg acgaaaacgt tcgtcgcgca tccgctcgat
     1981 ccagccgggc ctgcgttcat cgatttgctc ttgcgtccag ttgaagcaga tcatcgtgat
     2041 gtgagcaatc gcatcgatcg ccgcttcatc ataaccttct aacaattcaa ccgtgacatc
     2101 gggattgccc ggaatgtcca aagcatccag acctttacgc gccattgttg ccgcatgacc
     2161 cgccaagatc aaaccgtgct tctccaggcg ctggcccaga tcgattggtc tgtcgtcggg
     2221 cgccagccac cattgaaaac cgatgttgcg ttgtcgatga tattcgaggg tttcatcaat
     2281 tttccgatcg gcttcctctg ctgaccagcg cacgccgccg acaccattgc tgccgggatg
     2341 cggcagaccg ctgccgccca tgccgccgcg cagactccag cggccagatt catcggtgcc
     2401 gatccattca tacggatcgc tggaattcat catcgattca aagacgcggc gcagatcgtc
     2461 atccgctatg cttaaaggcg cgatccagcc tttgcgccgc gcgctgggaa tgggctctga
     2521 acaatacatc ctgatttgtt cgatcttgcc ttctttgatt ttcaagaatg ccgatcggtt
     2581 gtgctccacg taccgattgt tctcggtggc ctgaatgcga aattctaccg ccactaactt
     2641 atcatcaccg acgacattta atagttcgac tgacggatca gcccagcccg accattcttc
     2701 aatcaagcgt ttgacaacgc ggaactgcgg gcgatgaacc tcgccgccgt cccaacggcc
     2761 gatcaacatt ccaacatcgt ccgtaaaaat ttccgcgtat cgttcggccg tgttcgtagc
     2821 cagagccgtt aaaaaatctt gagcaagctg cttgagatcg gacatgacaa tctccaaaga
     2881 gaaaagatgt gacgaactaa ctgagtcggg gaagtgcggg gcgcatcgat caaaatctat
     2941 tcaagcgcta cgtcccgccg gagggttatt caacgaagat ctccacgcgc tcaccgtctt
     3001 gatcgtcggt gacatctatg atcttgccgc tggcgccgct cttgatggct tgcaccatct
     3061 cattatattt cgcgccttcg atgttaggcg caaagcgcgc gcccattttg agcgcgacgt
     3121 tgaccaaacc catggggatg ttgacgttga ccttgttttt acccgaagcc agatcggtga
     3181 cgcgcacgcg aaaccagcgc acatcgccgc cgacggtcgt cgaactcggc gagcgtttgt
     3241 cggctttgcc cgcgcttaag gcttgcagca gctttgaacc ttcttcggcg gagattttgc
     3301 cttcctggat catcttcagt atttgcattc gttcttcagc gcttgccatg tatcctccaa
     3361 aattttctta cccctcttca ggagaggcta gccaatcact acttgaattc gttcgccgtc
     3421 ttctgcttca ttcacatcga cgaccaacgg ttgatcgcgg ttgacgttgt ctttcat
//
LOCUS       DASRTJ010000623         3868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_284986,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000623 DASRTJ010000000
VERSION     DASRTJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284986"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFF70_12710"
     CDS             <1..800
                     /locus_tag="VFF70_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY45598.1"
                     /translation="MKGVNMETQTIDRRDFLKLLAASAGTIVGLQMIQNLTAAAQVAE
                     ASETGSSPHKWVMVIDQAKCTGCGQCTLTCRAHNDVPPSISWNRVIDAGKVGDREVFL
                     ARPCMHCEHAPCVEVCPVGASYRRADGVVMMDYDKCIGCRYCEVACPYGARAFNWEEF
                     TGPNPAVPTWGQPEIPRRPRGVAEKCSFCVQRIDRGLAMGLKPGVDQAATPACVVACP
                     VGARKFGDLNDPESEVSQLLADHAAYRLREDLGTAPRVYYLPARKGA"
     gene            803..2068
                     /gene="nrfD"
                     /locus_tag="VFF70_12715"
     CDS             803..2068
                     /gene="nrfD"
                     /locus_tag="VFF70_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZY45599.1"
                     /translation="MLAKSKIQLRLHLSKFQIWLLALLTLIVIGVIGGVIVMSRGLVV
                     TNLTDLVPWGLWITIDMSAIAMSAGAFLLCAATYLLGLKRLQPIARTATFIGLIGYSM
                     AVLCLLLDIGRPDRFWHALVYWNPHSVLWEVTMCVTLYLSVLMLEAAPIIGQTDWMQK
                     RLPKIARLMTSVHHYAPLLAVVGLGLSMLHQSSLGATYGVLKARPFWYRPDLSVLFMF
                     SAMAGGPALTTLISMLSARFTKRARVNDELLDLLSNFIGWVLVGYLYFRFWDAFSMTY
                     TYTPGRTEGLSLMTQGSLSFNFWVGEILLGAVIPIVILLKKNLRANMLLRMLALALVV
                     GGVVAYRWDTNLAGQLVMLTYLPQEIVARYTTYFPSIVEFMTGAGIVAFGVLALTLGV
                     RYLNVIDHRSAPQVEAESVAPLIASAATD"
     gene            2204..>3868
                     /locus_tag="VFF70_12720"
     CDS             2204..>3868
                     /locus_tag="VFF70_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZY45600.1"
                     /translation="MLENLRHWLIGLPLPTHRLAEERLNKIRALAAFSPDALSSIAYA
                     NQEIFLGLVVAGSAGLAFSWPIGVAIAVLLGVLALSYFQTLHAYPSGGGSYTVARENL
                     GSLPGLIAAAALMIDYVLTAAVSLTAGVAAIASAFPDLWPHRVEIALVLLLIILIANL
                     RGLRETGTLMAIPVYFFLIAYLSMLAIGIVRAFSEGPGSIGPAALAATAELTPFLILH
                     TFAAGCTALTGIEAISNGVPAFKRPESKNAGITLIVMACLMGVLFLGSIGLTQHFAVV
                     AGADETILSALARRILGSGILYVVVQVATLLILAVAANTSFADFPRVASILAHDGYLP
                     HQFSQLGDRLVFSNGMVVLAGLTGVLIVVFNGDTHALIPLFAVGVFIAFTLSQGGMVR
                     HWLRLRGAGWQVKALINGLGAGTTLITFFVVAISKFIEGAWIVAVLIPLIVFGFRAVK
                     AHYEQVGRELTLNGLPLSLRPWLEPRVVLPISGVHRGVLEALRFARSLSDQVTAVYVE
                     TNPAVTEKVKRDWEKWAPDVPLTIVPSPYRSMVGPLLDFLDQTDREY"
BASE COUNT          696 a         1022 c         1208 g          942 t
ORIGIN      
        1 ggatgaaagg tgtaaacatg gaaacgcaaa caattgaccg acgtgatttt ctcaaattgc
       61 tcgccgccag cgccggaacg atcgtcggct tgcagatgat tcaaaacctg acagccgctg
      121 cgcaagtggc cgaagcctcc gaaaccggat cgagtccgca caagtgggtc atggtgatcg
      181 atcaggccaa gtgcactggc tgcggccaat gcaccttgac ctgccgcgcg cacaacgatg
      241 tgccgccctc gatctcttgg aaccgcgtga tcgatgcggg caaggtcggt gatagagagg
      301 tctttctggc gcggccgtgc atgcactgcg aacatgcgcc gtgcgtggaa gtctgtccgg
      361 tcggggcaag ttaccggcga gcggatggtg tggtcatgat ggactacgac aagtgcatcg
      421 gctgccgcta ctgcgaagtg gcctgcccgt acggcgcgcg cgccttcaat tgggaagaat
      481 tcaccggccc aaatcccgcc gtgccaacgt ggggtcagcc cgaaattcca cgacggccgc
      541 gcggtgtcgc cgagaaatgt tcgttctgtg tccagcggat cgatcggggt ttggccatgg
      601 gcttgaagcc cggcgtcgat caagcggcca cgcccgcgtg cgtcgtcgcc tgcccggtgg
      661 gtgcgcgcaa gttcggcgac ttgaatgatc ctgaatcgga agtcagtcaa ttgctggccg
      721 atcatgcagc gtatcgactg cgtgaagacc tgggcactgc gccgcgcgtc tattatctgc
      781 ccgcgcgtaa aggagcatag tcatgctggc taaaagcaag attcaactgc ggctgcatct
      841 ctccaagttt caaatctggc tgctggcgtt attgacgttg atcgtcatcg gcgtgatcgg
      901 cggcgtgatc gtgatgagcc gcggactggt cgtgacgaat ctgaccgatc tggtgccgtg
      961 gggcttgtgg atcacaatcg atatgtcggc gattgctatg agcgcgggcg cgttcttatt
     1021 gtgcgccgca acttatttgc tcggcttgaa gcgcttgcag ccgatcgcac gtactgcgac
     1081 gttcatcggc ttgatcgggt acagcatggc cgtgttgtgt ctcctgctgg acatcggccg
     1141 gcccgatcga ttctggcacg ccctcgtgta ttggaatccg cattcagtct tgtgggaagt
     1201 cacgatgtgc gtgacgctgt atctgagcgt gctgatgctt gaggcggcac cgatcatcgg
     1261 gcagacggac tggatgcaaa agcggctgcc gaagatcgcg cgtttgatga ccagcgtcca
     1321 tcattatgcg cccctgttgg ctgttgtcgg tttgggctta tcgatgctgc atcaatcctc
     1381 attaggtgca acctacggtg tgttaaaggc gcggccgttc tggtatcggc cagatctctc
     1441 agtgctgttc atgttcagcg ccatggccgg tggaccggct ctgacgaccc tcatctcgat
     1501 gttatccgcg cgattcacca agcgtgcgcg cgtcaacgat gaattgctcg atctcttatc
     1561 gaacttcatc ggctgggtgt tggtgggtta cttatacttc cgcttctggg atgcgttctc
     1621 catgacgtac acttacacgc ccggtcgaac cgaagggctg agcttgatga cgcaaggttc
     1681 gttgtccttc aacttctggg tcggcgaaat cttattgggc gcggtcatcc cgatcgtgat
     1741 tctgttgaaa aagaatttgc gcgctaatat gttgctgcgg atgttagcgc tggcgttagt
     1801 cgtcggcggg gtggtggcct atcgctggga cacgaatctc gccggacagt tggtgatgct
     1861 gacttatctg ccgcaagaga tcgtcgcgcg ctacacgacg tacttcccct cgatcgtcga
     1921 gttcatgacg ggcgcgggca tcgtcgcctt tggagtgctg gcccttacgt tgggcgttcg
     1981 ttacctgaac gtgatcgatc atcgatccgc gccgcaagtt gaggccgaga gtgttgcccc
     2041 actgatcgcg tccgctgcga ccgattgatc tgtacgccga gatcgcgttc aagctaagtg
     2101 agacgccgca tcaatacgat gcggcgtttt tgtgttggtg cccgtttgtt cgtgcttggg
     2161 agcctgaacc gctaagtttc cgagcagcgg tataatcaaa cccatgttag agaacctgcg
     2221 gcattggttg atcgggctgc cgctccccac gcatcgatta gccgaagaac gcttaaacaa
     2281 aatacgcgca ctcgctgcat tctcaccgga tgcgctgtcc tcgatcgcgt atgcgaatca
     2341 agaaattttc ctcggtctcg tcgtagccgg atcggcaggc ttggccttct cctggccgat
     2401 cggcgtggcg atcgcggtgc tgttaggtgt gctggcgtta tcgtactttc aaacgctgca
     2461 tgcttatccc agcggtggtg gatcctatac cgtggcgcgc gagaatttgg gatcgctgcc
     2521 cggattgatc gccgcggccg cgttgatgat tgattacgtg ctgaccgcgg ccgtgagttt
     2581 gactgcgggt gtggcggcga tcgcttctgc gtttccagac ttgtggccgc atcgagttga
     2641 gatcgcattg gtgctgctgt tgatcatctt gattgcgaat ctgcgcggct tgcgcgaaac
     2701 aggcacgttg atggcgatcc cggtttattt tttcttgatc gcgtatctgt caatgctggc
     2761 gatcgggatc gtgcgcgcgt tcagcgaggg accaggctcg atcggaccag cggctctagc
     2821 ggcaaccgcc gaactcacgc cgtttctgat cctgcatact ttcgcagcgg gttgcactgc
     2881 gctgaccggc atcgaagcga tcagcaacgg cgtgccagcc tttaagcggc ccgaatcaaa
     2941 gaacgcgggc atcacgttga ttgtcatggc ctgcttgatg ggtgtgttgt ttctgggcag
     3001 catcggattg acacagcact ttgcagtggt ggccggcgca gatgaaacca ttctctcggc
     3061 tttggcgcgg cgaattctgg gttctggaat tttgtatgtt gtggtgcaag tagcaacgct
     3121 gttgatcctg gccgtggcgg cgaacaccag cttcgcagat tttccacgcg tcgcttcgat
     3181 tctggcgcac gatggatatc tgccgcacca gttctcgcag ttgggcgatc gattggtgtt
     3241 ctcaaatggg atggtggtat tggcgggttt gactggcgta ttgatcgtcg tgttcaacgg
     3301 cgatacgcat gccttgattc cgttgtttgc cgtgggcgtg ttcatcgcgt ttacattgtc
     3361 gcaaggcgga atggtgcggc attggttgcg attgcgcggt gcaggctggc aagtgaaagc
     3421 gctgatcaac ggactgggcg ccgggacaac gctgatcact ttcttcgtcg tcgcgatcag
     3481 taaatttatc gaaggcgcct ggatcgtagc agtgttaatc ccactgatcg tgtttggttt
     3541 ccgcgcggtg aaggcgcact acgaacaagt cgggcgcgag ttaacgttga acggtctgcc
     3601 actgtcactg agaccctggc tggagccacg ggtggtgctg ccgatctccg gcgtgcatcg
     3661 cggcgtgctg gaagcgctgc gctttgcgcg atcgttatca gaccaggtga cggcggtgta
     3721 tgttgagacg aatccggcgg tgacggagaa agttaaacgc gattgggaga aatgggcgcc
     3781 ggacgtgccg ttaacgatcg tcccgtcgcc atatcgatcg atggttggcc cgcttttgga
     3841 ttttctggac caaaccgatc gagagtac
//
LOCUS       DASRTJ010000624         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_131999,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000624 DASRTJ010000000
VERSION     DASRTJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131999"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFF70_12725"
     CDS             complement(<1..1248)
                     /locus_tag="VFF70_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZY45601.1"
                     /translation="MKLRLISITLFSAIVILFEIATAARPTHSSAAAPNVSAPAINSI
                     HLAGSTTYTIYFPIINNCYPLPSITDPYFVSGYQFDMSAINADWAWQHCSTGSPNIVV
                     AVIDTGVSLNHPDLNANLIGGYDFVDYDSIAQDGNGHGSNVAGIIGAALNGVGVVGVA
                     PNTHILPVRVLDDNGSGWSSDVIDGIVFAADNAQILNLSLGSTYGSILSSAIDYAVNT
                     RQRLVVAAAGNCGNSSYYLNGCSAQNQYVYPGAYSIDYPGGVIAVGAVNNYDNHASFS
                     NSDPYVTVSAPGVGIYSDYLGSGYASETGTSQATPHVAGLAALIWAKHPEYTAAQVEY
                     VITSTAVDLGPIGQDDDFGWGRIDVQAALNSIGVTAFQAASVSASIDLPHPIDHRTAK
                     IVAGQVLIKFKSGMKAAGIDWALS"
     gene            complement(1256..2473)
                     /locus_tag="VFF70_12730"
     CDS             complement(1256..2473)
                     /locus_tag="VFF70_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZY45602.1"
                     /translation="MIDKPDIVSTTAGPAISRFRGYASPLLHHLLLFGDRFSRLGTFA
                     LSIYIGVMTLIVIAAWSYAIDSISFGFVTGFGFLAFALADWLIIAALPRRHRSFGPIG
                     PGLIAFTFIRAVYTIIAAFINLSPLWTATLVQISNLALTGYFMDALWGEPFRLGVTRL
                     TLKSSKLNGAPPLRVLHFSDLHIERTTIREQKMLKLIDELQPDVIVYTGDLLNFSYLE
                     DAQARSDCQAILKQIQAPLGVYAIPGTPLIDSPEVLKSIYTGLDHIQLLINETQVIDG
                     YPFVQLIGLSCTHDPSLDAIQFDRLRPEVPSEKFSLLLYHSPDLMPEAIEAKIDLYLC
                     GHTHGGQVRLPIIGAIATSSIYGKRYEMGRYSQHNTTMYVSRGIGLEGKGAPRMRFLC
                     PPEIELIELRGEK"
     gene            complement(2612..3319)
                     /locus_tag="VFF70_12735"
     CDS             complement(2612..3319)
                     /locus_tag="VFF70_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZY45603.1"
                     /translation="MLYQLRDLQHRYGDRLVLDLPSIEIQRGEILSIVGPSGSGKSTL
                     LRLLQFLEQPTAGSITFDGTLIDRRPALEIRRRVATVFQRPLLLDRSVWENVAFGLQI
                     RRAQQIDQQVNFALDRVGLLPYAHTKARTLSGGEAQRVALARAIVIQPDVLLLDEPTA
                     NLDPYNVSLIENLIRDHDHLTIVLVTHNIFQAKRLADRVGLLLAGKLIELAPTDQFFN
                     VPSDPRTRSFINGEMVY"
     gene            complement(3498..>4242)
                     /gene="modA"
                     /locus_tag="VFF70_12740"
     CDS             complement(3498..>4242)
                     /gene="modA"
                     /locus_tag="VFF70_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY45604.1"
                     /translation="LSACTPAAPAQPTTLTVFAASSLTDAYNEIGTAFSADHPDVTVQ
                     FNFAGSQQLRTQLEQGAIADVFASANTKEMNTAITSNLIVSGTQQTFLHNRLIVILPK
                     NNPAAIQSLQDLAKPKLKIILAAPAVPVGGYALTALDKMNVEFGATFSMTVVANVVSY
                     EDNVKQVVAKIQLGEGDAGIVYSSDVTPAAAPQLSTIDIPDKYNVLATYPIAVLKAAP
                     HSQAATAFVEYVLSDKGQSILQKWGFLPK"
BASE COUNT         1016 a         1078 c         1208 g          940 t
ORIGIN      
        1 gctcagtgcc caatcgatgc ctgccgcctt catgcctgat ttgaatttga tcaatacttg
       61 ccccgctacg atcttcgcgg tgcggtgatc aattgggtga ggcagatcga tcgaggctga
      121 gacagaggcc gcctgaaatg ctgtcactcc aatcgaattc aacgcggctt gaacatcgat
      181 ccgtccccag ccgaaatcat cgtcttggcc gatcggtccc agatcaaccg ccgttgaagt
      241 gatcacgtat tctacttgcg ctgcggtgta ttcaggatgc ttcgcccaaa tcaacgcggc
      301 cagtccggcg acatgcggcg tcgcttgtga tgtgccggtt tcgctggcat aaccgctacc
      361 gagataatcg ctgtagatgc ctacgccggg tgccgagacc gtgacgtaag gatcagaatt
      421 tgagaagctg gcatggttat cgtagttgtt caccgcgccg acagcgataa caccgcccgg
      481 atagtcgatt gaatatgcgc cgggataaac atactgattt tgagcagagc agccatttag
      541 atagtagctg ctgttgccgc aattaccagc cgcggcaacg actagacgct gccgagtgtt
      601 gacagcataa tcgattgcgc tggacagaat tgagccgtaa gtgctgccta agcttaaatt
      661 gagaatctgc gcgttatcag cggcgaagac gataccgtcg ataacatcgc tgctccaacc
      721 gctgccgtta tcgtcgagaa cacgcacagg cagaatgtgt gtattcggcg ccacgccgac
      781 aacgcccaca ccattcaacg ccgcgccaat gatgccggcc acattcgatc cgtgaccatt
      841 cccatcttgt gcaatgctat catagtcgac gaaatcataa ccgccgatga ggttggcgtt
      901 cagatcggga tgattgagac tcacgccggt atcaatgaca gcgacgacga tattcggact
      961 gccggtgctg cagtgctgcc aggcccaatc ggcgttgatc gctgacatgt caaattgata
     1021 accactcaca aaatacggat cggtgatcga tggcagcggg taacaattat tgatgattgg
     1081 aaagtaaata gtataagtgg ttgagccggc caaatggatc gaattgatcg ccggagcaga
     1141 aacgttgggc gcggctgccg aggaatgagt tggcctagct gcagtcgcga tttcgaacaa
     1201 gatgacgatg gcgctgaaga gggtgatcga tattaggcgc agtttcaaga tctgatcact
     1261 tctcccctcg caattcgatg agttcaattt ccggcggaca caagaagcgc atgcgcggcg
     1321 cacctttgcc ttccaggcca atgccgcggc ttacatacat ggtcgtgttg tgctggctgt
     1381 agcgccccat ttcatagcgc ttgccataga tcgagctggt cgcgattgcg ccgattatcg
     1441 gcaggcgcac ttgtccgcca tgcgtgtggc cgcacaaata aagatcgatc ttggcctcga
     1501 tcgcttccgg catcagatcg ggtgaatgat acagcagcag tgagaatttc tccgatggaa
     1561 cttccgggcg cagccgatcg aactggattg catccagcga tggatcgtgg gtacagctca
     1621 ggccgatcag ctgtacgaaa ggatacccat cgatcacttg cgtttcgttg atcaaaagtt
     1681 gaatgtgatc gagtccagta tagattgatt tcaggacttc aggtgaatcg atcaagggtg
     1741 tgccgggaat cgcgtatacg cccagcggcg cttgaatctg tttcaggatc gcttggcaat
     1801 ccgatcgggc ttgggcatct tccagatatg aaaagttgag cagatcgccg gtgtagacaa
     1861 tcacatcggg ctgcagttca tcgatcagct tcaacatttt ttgttcacgg atcgtagtgc
     1921 gctcgatgtg cagatcgcta aagtgcagga cgcgcagcgg tggcgcgccg ttgagcttgg
     1981 acgatttaag cgtcaagcgc gtgacaccca agcgaaaagg ctcgccccac agagcgtcca
     2041 taaagtagcc ggtcaatgcc aagttgctga tttggaccag cgtcgcagtc cataacgggc
     2101 taaggttgat aaacgcagca atgatggtgt agactgcgcg aatgaaagtg aaagcgatca
     2161 agcccggccc gatcggcccg aacgatcgat ggcggcgcgg cagcgcggct atgatcagcc
     2221 aatcggctaa cgcgaaagcg aggaaaccga agcccgtcac gaagccgaag ctgatcgagt
     2281 cgatcgcata tgaccaggcc gcgattacga tcaaggtcat gacgccgatg taaatcgaca
     2341 aggcaaaggt gcccaggcgg ctgaatcgat caccgaacaa tagcaagtga tgtaacagcg
     2401 gcgaggcgta gccgcgaaag cgggagatag ctggcccagc ggttgtcgag acaatatcag
     2461 gtttgtcaat cacaatcgaa tgatactcgt caccgtgttg attggcaagc ctcatctgac
     2521 cacccaccct cttccctctc ccaagttcaa aaatctacga cgatgattgg gagagggaag
     2581 aagggcacaa gactgatggc tgaggcgggg gttagtacac catttcgccg ttgataaacg
     2641 atcgggtgcg cggatcgctc gggacgttga agaattgatc ggttggggcg agttcgatca
     2701 acttgccagc gagcagcaag ccaacccgat cggccaggcg tttggcttga aagatattgt
     2761 gcgtgactaa gacgatcgtc aggtgatcat gatcgcggat caagttttcg atcaagctga
     2821 cgttatacgg atcgagattg gcggtgggtt catccagcag caagacatcc ggctgaatca
     2881 caatggcccg cgccagcgct acacgctgag cttcgccacc ggacaaggtg cgcgctttgg
     2941 tgtgagcgta tggcaacaaa cccactcgat ctagcgcgaa atttacctgc tgatcgatct
     3001 gctgcgcccg acggatctgt aacccgaacg cgacgttctc ccacaccgat cgatcgagca
     3061 gcagcgggcg ttgaaaaaca gtggcgacgc gtcggcggat ttccaaggcg ggtcgacgat
     3121 cgatcaacgt gccatcaaat gtgattgatc cagccgtggg ttgttcaaga aattgcagca
     3181 gtcgtaaaag cgtagacttc cctgatccgc tggggccgac gatcgataag atttcgccgc
     3241 gttgaatctc gattgagggg agatcgagca cgagtcgatc gccgtaacga tgttgaaggt
     3301 cgcgtagttg atagagcata tcaatgaaca attttcattt tgcaatgaat aaaggaaggc
     3361 gcaagcatcc tgagcggagc gatgaactcg aagaggtcac tgcgcagtcg aaggacgcgc
     3421 tcgttgcaac attcgcgtcc ttcgactctg atgggaatct tggagattcc catctccgct
     3481 caggatgctt ccacatctca cttcggcaga aagccccact tttgcaagat cgattgaccc
     3541 ttgtctgaaa gaacatactc aacaaaggcc gttgccgctt gtgagtgtgg cgctgccttc
     3601 aagaccgcga ttggatacgt ggctaagacg ttatatttat caggaatatc gattgtgctg
     3661 agctgcggtg cagcggcagg cgtcacatcc gacgaataca cgatgcccgc atcgccttcg
     3721 ccgagttgta tcttggcgac gacttgttta acgttatcct cgtatgaaac gacgttggct
     3781 accacagtca ttgaaaacgt tgcgccgaac tcgacgttca ttttatcgag tgccgtcaag
     3841 gcatagccgc cgacgggcac agctggcgcg gcgaggatga ttttcaattt gggtttggcc
     3901 aggtcttgaa gcgattgaat ggctgccgga ttgttcttcg gcagaatgac gatcaggcga
     3961 ttgtgcaaga atgtttgctg agtcccgctg acaatcagat tcgatgtgat ggcggtattc
     4021 atttctttcg tattggctga agcgaacaca tcggctattg cgccttgttc caattgcgtg
     4081 cgcagctgct gcgatccggc aaagttgaac tgaactgtca catccggatg atcggcgctg
     4141 aaggcggtgc cgatttcgtt ataggcatcg gtcagtgacg aggccgcaaa caccgtgagc
     4201 gtcgtcggtt gagcgggcgc agcgggtgtg caggcgctca ac
//
LOCUS       DASRTJ010000625         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_132014,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000625 DASRTJ010000000
VERSION     DASRTJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132014"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFF70_12745"
     CDS             <1..634
                     /locus_tag="VFF70_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HZY45605.1"
                     /translation="TVRYFANTAKIVELRRTWQSQDNYIQSFIRPTTRNIIYNTASRY
                     KVEEIYGALRAQLQKEIEDQLRIQFDQEGLVLDAFQLRNVTFSPEYAQSIEQKQIAQQ
                     QSEQAKLLVQKAQQEADQVRATAQGLADAAVTKAKGDAEALRQIAAALKDNPELLMYT
                     YIQKLAPNVGLILLPSGGSNPFIIDLKQLQAEFSQAPAASSSGPITPTVP"
     gene            900..1961
                     /gene="pdhA"
                     /locus_tag="VFF70_12750"
     CDS             900..1961
                     /gene="pdhA"
                     /locus_tag="VFF70_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZY45606.1"
                     /translation="MATTKTSTAPVRDKKSTNGRANGEADRSTEGSVVDTTTLKSWYR
                     EMVLIREFEERARQSYQQGKIGGFLHLYIGEEAVAVGAIHALKPQDHIVTHYRDHGHA
                     IARGLDPKKLMAELYGRVDGTAHGRGGSMHFADTSKNFWGGYAIVGGHLTIAVGLAIA
                     AQRAKEDRVALAIFGDGSTDAGQFYECLNLAAVWKLPVVFLCENNQYGMGVAIQKASA
                     VTEVYKKASMANIPAVQVDGMDVVKVHEVMKQVIEEVRNGGGPRFVEALTFRYQGHSV
                     ADPDKYRAKEEIDKWKKRDAIEHVRALLIDKHSVKADELAAVDADVQHEMDEVVKFAD
                     ASAAPDVSTIYDYIYAQPV"
     gene            2021..2992
                     /locus_tag="VFF70_12755"
     CDS             2021..2992
                     /locus_tag="VFF70_12755"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZY45607.1"
                     /translation="MAVITIREALKQAMREEMLRDERVFLLGEDIAAYGGAYAVTKGL
                     VEEFGEERVRDTPISEGIIVGAAMGAAVGGQRPVAELMTINFAILAMDQLINHAAKMH
                     YMFGGQWNVPMVVRTAAGWGQLSATHSQTLEVFFAHVPGLKVIFPGTPYDSKGLLKAA
                     IRDADPVVFIEHTALYGVKGEVPDGDYVLPIGKSDIKREGQHCTIATYGRMLQVSLEA
                     ATTLAADGIEVEIVDLRTLRPLDMAPVYESVQKTNHAVVVTEEWVSFGVGAEVAARIS
                     QDAFDYLDAPVGRVGSLEVPMPYAKNLEQAALPWPKDVVQAVKAALA"
     gene            3003..4286
                     /locus_tag="VFF70_12760"
     CDS             3003..4286
                     /locus_tag="VFF70_12760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZY45608.1"
                     /translation="MATKVTMPQMGFDMTEGKVARWLKKVGEAVKQGEPVAEIETDKV
                     NIETEAVGNGVLREIIASEGSTVPVGTLIAIIAEPDEDISALIAESGKGSATAPKATV
                     VQEAAAVSTPALASTSTVPMVDPSATHVASKGNGRLKASPLAKRVARERGVNISDVTG
                     TGPGGRIVKRDVDSAVSMPIAAPSALRVAAPAMLGPSLLKDELVAPSKLRQTIARRMT
                     ASKQTVPHFYVTIEVEMSEALALRSKLNAIVGDDGKISVNDMVIKAAALALTHFPSIN
                     ASYTDSGIQRHGSVNIGIAVALDQGLITVVVNEADRKPLVQIAREARDIATRAKNGKA
                     RPEEMQGSTFTVSNLGMFGEVDSFTAIINPPEAAILAVGTMRDVPIVRDGQIVPGKTL
                     KMTIAADHRVTDGAEAAKFMQVLKQNLEEPLRLLV"
     gene            4582..5511
                     /locus_tag="VFF70_12765"
     CDS             4582..5511
                     /locus_tag="VFF70_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZY45609.1"
                     /translation="MTKIQSRFGLMLAGVALAFITASLTVQATSGILIIPPTLSGVIT
                     LPGGSLPVPVGTKAMLLNLDRSIKGTSVVSITTGAYTFPVIEPGVYLIRGEPPIGSLT
                     YGPSDLIAVHVLTQAVTVPTLTLSTPSVSGTVYLPDGFTPANSSVEVFTSPHGIPVLV
                     EVRSALSGTFVIGGLPTGTYTLKAIPFPNLPYWKSVPIDVLIDPGAPQYISLTLRPAS
                     VYGLVKDALNFPVQNAVVHAVDANGHHRYDVSGSLGVFAQDKQMNNAPDRVTRSGVCA
                     SVFSRATSIHSWANSVARSLISFWLRVMGRSQM"
BASE COUNT         1318 a         1400 c         1679 g         1241 t
ORIGIN      
        1 gacggttcgc tacttcgcca acacggccaa gattgtcgaa ctgcggcgga catggcagag
       61 ccaagacaac tacatccaaa gtttcattcg accgacgacg cgcaacatca tctacaatac
      121 ggcctcgcgc tataaagtgg aggagatcta cggcgccctg cgcgcgcagt tgcagaaaga
      181 gatcgaagat caactgcgta ttcaatttga tcaagaaggt ctagtcttgg atgcgttcca
      241 gctgcgcaac gttactttct cgccggagta cgcgcaatcg atcgagcaga agcagattgc
      301 gcagcagcaa tctgaacagg cgaagctgct ggtgcagaaa gcgcagcagg aagcggatca
      361 ggttcgcgcg acggctcaag gtttggcgga tgcggctgta accaaggcca aaggcgatgc
      421 cgaagcgctg cgccaaattg cggcggcgct gaaagacaat cctgaattgt tgatgtacac
      481 ttacattcag aaacttgcgc cgaatgtggg tttgattctg ctgccctccg gcggcagtaa
      541 tccgttcatc atcgatctga agcagttgca ggccgagttc agtcaagcac cggcggcaag
      601 cagtagcgga ccgatcacgc cgacggtgcc gtaagatttg tatttgatcg ctccacagcc
      661 gatcgggttt gctgaccgat cggctgttga tttttttatc gatcggccta taatcaggca
      721 cttacccaat ccgatcgggc tgcgtaacct gatcggattt ggcgggttct agtgggttga
      781 ccgcactgaa aatatcgatt gtacgatcga cgccctgcgg cgtcgttttt acgaacgcaa
      841 tcgcatatca gtttgcagag tatcgagccg aaaaagttag aacaagggaa ggagttaaca
      901 tggcaacaac gaaaacgagc accgctcccg tgcgcgacaa gaaatcaacc aacggacgcg
      961 cgaacggcga agcggatcga tccacggagg gcagtgtggt tgatacaacg acgttgaaga
     1021 gttggtatcg cgagatggtg ttgattcgcg aatttgaaga gcgcgcccgg cagagttatc
     1081 agcagggcaa gatcggcggc tttctgcatt tatatattgg tgaagaagcc gtggcggttg
     1141 gcgcgattca tgcgctgaag ccgcaagatc atattgtgac acattaccgt gatcacggtc
     1201 atgcgattgc gcgcggcctc gatccgaaaa aattgatggc cgaactctat ggccgcgtcg
     1261 acggcacggc gcatggccgc ggcggctcga tgcactttgc ggacacgagc aaaaactttt
     1321 ggggcggcta tgccatcgtc ggcgggcact tgacgattgc ggttggcctg gctatagccg
     1381 cgcagcgcgc aaaggaagat cgagtggcgt tggcgatctt tggcgatgga agtacagacg
     1441 ccggacaatt ctacgagtgc ttgaatttgg cggccgtgtg gaaactgccg gtggtgttct
     1501 tgtgtgagaa caatcaatac ggcatgggcg ttgctatcca aaaagcctcg gcagtgacgg
     1561 aagtgtacaa gaaggccagc atggctaata tccccgccgt gcaagtcgac ggcatggatg
     1621 tagtgaaagt ccatgaagtc atgaagcagg tcatcgaaga agtgcgaaac ggcggcgggc
     1681 cgcgcttcgt cgaagcgttg accttccgct atcaaggcca ctcggtggct gatccggata
     1741 agtatcgcgc taaagaagag atcgataaat ggaagaagcg tgatgcgatc gagcacgtgc
     1801 gcgcgctatt gatcgataag catagtgtga aagccgatga gctagcggcc gttgatgcgg
     1861 atgtgcagca tgagatggat gaggtcgtca agtttgcgga tgcttccgcc gcgcccgacg
     1921 tttcgacgat atacgattac atttacgccc agccagtgta aatcaagctg acaagtgttg
     1981 ggcaagttgt caacttgcct tacatacaaa ggaaacgatt atggcagtca ttacaatccg
     2041 agaagcgttg aagcaagcca tgcgcgaaga gatgctgcgc gatgagcgag tgttcttgct
     2101 cggcgaagac atcgctgcgt atggtggtgc ctatgcggtc acaaaaggtt tagttgaaga
     2161 gtttggcgaa gagcgtgtgc gcgatacgcc tatcagtgaa ggcatcatcg tcggcgcggc
     2221 gatgggcgcg gccgtcggtg gtcaacgccc tgtggcggag ctcatgacga tcaactttgc
     2281 gatcctggcg atggatcaat taatcaatca tgcggccaag atgcactaca tgttcggtgg
     2341 gcagtggaat gtgccgatgg tggtgcgaac cgccgctggg tggggacaac tctcagccac
     2401 tcactcccaa acgctggaag ttttctttgc gcatgtgccc ggcctgaaag taattttccc
     2461 cgggacgccg tatgattcaa aaggtttgct caaagcggcg atccgtgatg ccgatccggt
     2521 ggtgttcatc gagcacactg cattatatgg ggtgaaaggt gaagttcccg atggggatta
     2581 tgtgctcccg atcgggaaga gcgacatcaa gcgtgaaggc cagcactgca cgatcgcgac
     2641 gtatggccgc atgctgcagg tttcgctcga agcggcgaca acactcgctg ccgatggcat
     2701 cgaagtggag atcgtcgatc tgcgcacact acgtccgctg gatatggcgc ccgtgtacga
     2761 atcagtgcag aagaccaatc acgctgtcgt cgtgacggaa gagtgggtga gctttggtgt
     2821 aggggcagaa gtagccgcgc gcatttcgca agacgctttc gattatctcg atgcgccggt
     2881 cggacgggtg ggcagtttgg aagtgcccat gccgtatgcc aagaacctgg aacaagcagc
     2941 tctaccgtgg ccgaaagatg tagtccaagc ggtcaaagct gcactagctt aaggggtgag
     3001 atatggcgac taaagtaacg atgccccaaa tgggcttcga tatgaccgaa ggcaaagtcg
     3061 cgcgctggct gaagaaagtc ggcgaggcgg tgaagcaggg cgagccggtg gccgagatcg
     3121 aaactgacaa ggtcaatatc gagaccgaag cggttggcaa tggtgtattg cgcgaaataa
     3181 ttgctagtga aggctcgacc gttccggtcg ggacattgat cgcgatcatt gccgaaccgg
     3241 atgaagatat ttcggcgttg atcgccgagt cgggtaaagg atcagccact gcgccaaagg
     3301 caactgttgt tcaagaagca gctgccgttt caacgcccgc acttgcttcg acatctaccg
     3361 tgccgatggt agacccatcg gccacgcatg tggcgagcaa aggtaacggt cgattgaaag
     3421 cctcgccgtt agccaagcgc gtggctagag agcgcggcgt caacatatcc gatgtgaccg
     3481 gcactggccc cggcggccga attgtcaaac gcgatgtcga ttccgctgtc tcgatgccaa
     3541 tagctgcacc atctgcgctg cgtgtcgctg cacctgcgat gttgggtccg tcgctattga
     3601 aagatgaact cgttgcacca tccaagctgc gccaaacgat cgcgcggcgg atgacggcca
     3661 gcaaacaaac ggtgccacac ttctacgtca cgatcgaagt tgagatgagt gaagcgctgg
     3721 ccttacgatc gaaattgaat gcgattgtcg gcgatgacgg caagatcagc gtcaacgata
     3781 tggtgatcaa agccgcggct ttggcactga cacacttccc atcgatcaat gcttcgtata
     3841 ccgatagtgg tattcagcgg catggctcgg tcaacatcgg catcgccgtc gcgcttgatc
     3901 aaggcttgat cactgtggtc gtcaacgaag ccgatcgcaa gccgctggtg cagatcgcgc
     3961 gtgaggcacg tgacattgcg acccgtgcca agaacggcaa agcgcggcct gaagaaatgc
     4021 agggcagcac gtttactgtc agcaatctgg gtatgttcgg tgaggtcgac tcgttcaccg
     4081 cgatcatcaa tccgcctgaa gccgcgatcc tcgccgtggg caccatgcgt gatgtgccga
     4141 tcgtgcggga tggacagatt gtgccgggca agactttgaa gatgaccatt gccgccgatc
     4201 atcgcgtgac cgatggcgcg gaagcagcca agttcatgca agtgctgaag cagaatctgg
     4261 aagagccgct gaggttgttg gtgtaattgt aaattagacg ttggatattg gaagttagcg
     4321 ccgccgatcg aatttcgatc ggcggttttc gtttcttgcc cctcgctgaa gagctgcgta
     4381 ggcaattagt cctaaaagag ctgatagcag ccaatgtttt acacagcgtt tacactcgcg
     4441 ctacttagat taaacgcaga tgagcgatga tacagttgcg agagcaaatt tcgttagctg
     4501 gaggaagagc ttggcttgta atagtacatt ggcatcagcg ttgtgtaggg caaccccttg
     4561 tattaaactc gaaaggagcg tatgacaaaa attcaatctc gatttggact gatgttagca
     4621 ggcgtggcgc tggcgtttat cactgcgtct ttgaccgtgc aggctacttc gggaatcttg
     4681 atcattccac cgacgctgag tggcgtgatc actttaccgg gcggatcgct gccggtaccg
     4741 gtgggcacaa aagcgatgct attgaatctc gatcgatcga tcaaagggac tagcgtcgta
     4801 tcgatcacga ccggcgctta cactttccca gtgatcgaac cgggcgtcta tctgattcgc
     4861 ggtgaacccc cgatcggatc actcacttac ggtccgtccg atctgatcgc ggtgcatgtg
     4921 ttgacgcaag cggtgactgt gccgaccctg actttatcga cgcccagtgt atcaggcacg
     4981 gtgtatttgc ccgatggctt cacgcccgcg aattcgtctg ttgaagtttt cacttcgcca
     5041 cacggcattc ccgtcctggt tgaagttcga tcggcgctga gtggtacgtt tgtgatcggc
     5101 ggattgccaa ctggcacata taccctgaaa gccataccgt ttcccaatct accgtattgg
     5161 aaatccgtgc cgatcgatgt gttgatcgat ccgggcgcgc cgcaatacat ttcattgact
     5221 ttgcggccgg ctagcgtcta tggcttagtg aaagacgcgt tgaattttcc ggttcaaaat
     5281 gcagttgtcc acgcggtcga cgcgaacggt catcatcgct acgatgtcag cggctcactg
     5341 ggtgtgtttg cccaggacaa gcagatgaac aacgcccccg atcgggtgac tcgatcgggt
     5401 gtttgcgcct cggtcttttc gcgtgcaaca tcgatccatt cgtgggcaaa ctcggtggcg
     5461 cggtctctaa tttctttttg gcttagagtc atgggccgat ctcagatgta gcagggtttg
     5521 gaatcaaagt atagttcgat tcaacctcaa gtcaaacctc tggcagtagg ttgctaattg
     5581 catcgaccct cacatgcctc tccctaaaag ggagaggaga agcggcgttg atcgatag
//
LOCUS       DASRTJ010000626         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_360184,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000626 DASRTJ010000000
VERSION     DASRTJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360184"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..883
                     /locus_tag="VFF70_12770"
     CDS             29..883
                     /locus_tag="VFF70_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZY45610.1"
                     /translation="MSEQLTDSGSIAEEMISADPIGDLAPKKDRSPRVNRWVVLAAFL
                     IGIVIGAFGFAAYNIFSNGRAGDDKSILREAARNGFMDAVATLNAGGNPSAVASNDST
                     APASTAFQVREANRLGDKNAKVTIIEFADFQCPYCERWFQQVETQIVQQYVQTGKITF
                     VYKHSAFLGQESEWAGTAAECAADQNKFWEYHDVLFKQQNGENQGAFTKDKLIGFAQS
                     LELDMSKFTPCLQNDQTLDRVQADTHEGQQASVNGTPTFFINGQRIVGAQPYSTFQAA
                     IEKALSAQ"
     gene            complement(964..2571)
                     /locus_tag="VFF70_12775"
     CDS             complement(964..2571)
                     /locus_tag="VFF70_12775"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY45611.1"
                     /translation="MSIRSIRALAVLVVLPIVLLLLSGLFSSTVRASTSTIKHVANTN
                     NSGVGSLRDAIGSASFGDTIIFDPSVVGTIYLSPTNIFIGIDLTISGPGASVLSISGS
                     NNNGVFNVASANVTISGLTIRNGSASTGAGIINSGIFGVLTITQCVIQYNMATTGAGG
                     GIANSGNLVIDNSRIDDNYAFASNGGGLYSAAGSVTLINVTIDSNQAGMGGGVSLEST
                     QAQLISSHVLTNTSITSGGGIYIDANSTLDLEYSRIANNTAGNSAIGGGIFNVGETDL
                     FYTTIISNGTGTSGAGGGLGNSGILNLTRSSVLSNTAEADGGGILNVSPGVVDLYYST
                     LAYNVANGTAGGILNSNAIVTATDSTLSGNQAYGLFNNGAAIRNVGVGKIWLYYDTIS
                     YNNGGSNGGLSIEGSSVITAVGTIIAGNSGATNPDCSGNLNSLGHNLITVDTGCTING
                     TITGNIIGIDPQLLSLQDNGGSTWTLALQSTSPAIDAGSASYCPSYDQRGIHRPIDGN
                     HDGVATCDIGAYEVQIYVYLPLVAKNS"
     gene            2715..>2921
                     /locus_tag="VFF70_12780"
     CDS             2715..>2921
                     /locus_tag="VFF70_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZY45612.1"
                     /translation="MASWSRREFLKFAGVTLAATVLKPTPPEEHALGRPTGFGRVTNY
                     SVWSYNNPQPGAERVKAYYRDDVLP"
BASE COUNT          703 a          723 c          819 g          676 t
ORIGIN      
        1 cgaaggatat tcgagaggat gattttgagt gagtgaacaa ctaacagaca gtggatcgat
       61 cgcggaagaa atgatctcgg cggacccaat cggcgatctc gcgccgaaaa aagatcgatc
      121 gccacgcgtg aaccgatggg tggtgttggc cgccttcttg atcggcatcg tgatcggcgc
      181 atttggtttt gcggcctaca atattttcag taatgggcgg gcgggcgatg acaaatcgat
      241 cttgcgtgaa gcggcccgca acgggttcat ggatgctgtc gccacgttga atgcgggcgg
      301 taacccgtcg gcagtggcga gcaacgattc aaccgcgccc gcctcaacgg cctttcaggt
      361 gcgggaagcc aatcggttgg gggataaaaa cgccaaagtg accattatcg agtttgcaga
      421 ttttcaatgt ccatattgcg agcgctggtt ccagcaggtt gaaacacaga tcgtgcagca
      481 gtatgtgcaa actggcaaaa tcacgtttgt ctacaaacat tctgcttttc tgggacaaga
      541 atcggaatgg gccgggacgg cggccgagtg cgccgccgat caaaataaat tctgggaata
      601 ccacgatgtc ttattcaagc agcagaatgg ggaaaaccaa ggtgctttca ccaaagataa
      661 gttaatcggt ttcgcgcaaa gtttggaact cgacatgagc aaatttacac cgtgcctaca
      721 aaacgatcag acactcgatc gagttcaggc cgatacccat gaaggtcagc aagccagcgt
      781 gaatggcacg ccgacgttct tcatcaacgg tcagcgcatt gtcggcgcgc agccctacag
      841 cacgttccag gcggcgattg agaaagcgct gagcgctcaa taagttgttg atgttgttaa
      901 gacgtttggt gttattgacg caaaaagaaa ccggagaggc tgcttaagtc tctccggttt
      961 agtttacgaa ttctttgcga ccaacggcaa gtaaacgtag atttgcactt cataagcgcc
     1021 tatgtcgcac gtcgccacac catcgtgatt gccatcgatt gggcgatgga taccacgctg
     1081 atcatatgaa gggcagtaac tggccgaacc cgcatcgatg gccggactcg tcgattgcaa
     1141 ggccagcgtc cacgtcgagc cgccgttgtc ttgcaaagac aataattgcg gatcgatgcc
     1201 aatgatgttg ccagtgattg tgccattgat cgtgcagccg gtatccactg tgatcaagtt
     1261 gtggccgagt gaattcaaat ttcctgagca atccgggttt gttgccccgg aatttccggc
     1321 gatgatcgtg ccgacggccg tgatgacact gctgccttcg atcgacagtc caccgttgct
     1381 gccgccgttg ttgtaagaga tcgtgtcgta gtacagccat attttaccta cgcctacatt
     1441 tctgatcgct gctccattat tgaacaaacc gtaggcttga ttgccgctga gggtgctatc
     1501 ggttgcggtg acgatcgcgt tgctgttcaa gatgccgcct gcagttccat tcgcaacatt
     1561 gtaggcgagc gtgctgtaat acagatcgac tacgccgggg ctgacgttca agattccgcc
     1621 accgtcagcc tcggcggtat tgctgagcac gctgctcctc gtcaaattca gaatgccgct
     1681 gttgccgagg ccgccgcccg cgccgctcgt gcccgtgcca ttgctgatga ttgtggtata
     1741 gaaaaggtcg gtttcaccga cgttgaaaat tccgccgccg atggcagagt tgccggctgt
     1801 gttgttggcg atgcggctgt attcaagatc gagcgtgctg ttcgcatcga tatagatacc
     1861 gccgccgctg gtgatgctgg tgttcgtcag cacgtgcgaa ctaatcaact gggcttgggt
     1921 cgattcgagc gagacgccac cgcccatacc ggcttgattg ctatcgatcg taacgttgat
     1981 caaagtcacg ctgcccgccg cgctgtataa tccgccgccg tttgaggcga aggcatagtt
     2041 atcgtcgatg cggctgttgt cgatgaccag attgccggag ttggcaatgc cgccgcctgc
     2101 gccggtcgtg gccatattgt attgaatgac gcactgcgta attgtcagga cgccaaaaat
     2161 tccggaattg attatccccg caccggttga tgcacttcca ttgcggatcg tcaaaccgga
     2221 gatggtcaca ttggccgagg caacattgaa gacgccatta ttattgctgc cgctgatcga
     2281 taagacactt gcgcccggac cgctaatcgt tagatcgata ccgatgaaga tattggtcgg
     2341 ggacaaatag atcgtgccga cgacgctggg atcgaaaata atggtgtcgc caaatgacgc
     2401 agagccgatt gcgtcacgca gcgaaccgac gccgctgtta tttgtattcg cgacatgttt
     2461 gatagtcgag gttgaagcgc gaactgtcga agaaaaaagt ccgctaagca atagcagaac
     2521 aatcgggagg acaaccagaa cggcgagcgc gcggatcgat cgaattgaca tgaaagccac
     2581 ctctatgcat atcggacgcc acgtgaatcc gattatagca tcaatgttaa acccatactt
     2641 aagtatcctt ggttatcgat gaaagttcgg gtatacttaa gtaacaactt agaaaaggca
     2701 aaggacagag tgtcatggcg agttggagta gaagggaatt tctaaaattt gctggggtaa
     2761 cattagccgc gacggttttg aaaccgactc caccggaaga acatgcgctg ggccggccca
     2821 cgggttttgg acgggtcacc aattattccg tgtggagcta caacaatccg cagcccggcg
     2881 cggaacgggt caaagcgtat tatcgtgatg atgtgttgcc g
//
LOCUS       DASRTJ010000627         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_360558,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000627 DASRTJ010000000
VERSION     DASRTJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360558"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFF70_12785"
     CDS             complement(<1..331)
                     /locus_tag="VFF70_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZY45613.1"
                     /translation="MLNPIGPVALSILIGAALFGFLSGFRDSANIVATMISSRAIGPR
                     RALFIAAASAGIGPFIFGLSVATTIGRDFILPAIASPPVIIAALIAAIVWNTFTAWLG
                     LPSSSMHA"
     gene            complement(462..971)
                     /locus_tag="VFF70_12790"
     CDS             complement(462..971)
                     /locus_tag="VFF70_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45614.1"
                     /translation="MAVRSLNPIELAEVNALFGDGLDLQRVRIKEESSIGNIVGRIGA
                     WLKRLPPPAANAITVGNVSYFPRKLTGDLTDMGWLMHELTHQWQYQHVGIQYLFQAAF
                     APTYVYEAAGQRPSDAVKELSEKGQKFSDFNREQQGDIVRDYYFALKQNEDVSGWQNY
                     LKEIRTRAR"
     gene            complement(1107..1295)
                     /locus_tag="VFF70_12795"
     CDS             complement(1107..1295)
                     /locus_tag="VFF70_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45615.1"
                     /translation="MPDEWKVGDRFLLRSGRAVGTVIGVGKDHIKIQYDNGQVADIDP
                     RDILHNVKPEPDPDEEDE"
     gene            1760..2983
                     /locus_tag="VFF70_12800"
     CDS             1760..2983
                     /locus_tag="VFF70_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45616.1"
                     /translation="MRRWQTVWSVILLGLASVASLMLTLGVSGARPAGAARSIAGVDD
                     QSGPTLVSSTGKIAYVFQSDTAPNDQANMAFLNSRGYTVTPVLLSFVSSTDFSPFDLI
                     MIGEGTGTLNEWGTVAADVPAIIAPNKPIMGLSEGGYAFFGKLSLFIGWPRGWHSTDL
                     VMTRVLTAPNAMFTTPNLVSGSPITAYTQNITEVAIYLAAKPADVVAFAEETPSTNHA
                     MLISQGCRYLWGFGGSPLIMTAAGQNLFQNTVEYALHSNCPPPPPLPTGECVTISKAS
                     APAGNTPVMPGDTIHYTLTLLLKNILSCQNQTGLVIDRVPTDTLVISNSLGQGGYLAQ
                     DNAVVWQVSPAAAPFTRTFSVNVSLLSCLHTPLVINAATLGLTNTVSVKSNVVQHPVI
                     CPTTIFNIYLPLSLK"
     gene            3143..4198
                     /locus_tag="VFF70_12805"
     CDS             3143..4198
                     /locus_tag="VFF70_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZY45617.1"
                     /translation="MNKRFLLLLSSVVIMFIMIWMFIGSQPAVSASTPSRRSSRLDTV
                     TRLTDRALLTSTLAFTAYLPLVIKQPAAPPATNWLDYVNYYRALANLPSVTENPIWSD
                     GDWKHGRYMVKNDFIGHDEDPANPWYTPEGRTAAQNGNTMVSSSATVTDSYAIDFWMR
                     APFHAVGILDPALGQIGFGSYREADGGWQMGATLDVIRGLGAIPPAVAFPIEYPGDGA
                     TIGLRSYTGGEWPDPLSSCAGYTTPSGLPIILQIGPGNLMPSVTTHSFKQSTTSLEHC
                     IFDETNYVNPDSTTQGVGRSVLNARDAIVLIPRSPLTAGATYTVSIATNGQTYTWAFT
                     VASTATVNEFAGPLSMR"
     gene            4365..>4755
                     /locus_tag="VFF70_12810"
     CDS             4365..>4755
                     /locus_tag="VFF70_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45618.1"
                     /translation="MFRTIERLALVFIAAIIIVLIAIGIVRGAPQSGVVGTGTALSCD
                     EAAFDARLAGGGLITFNCGAAPVTITLSSEIPITATTVISGNGLITLSGNDATRLFNV
                     GSGVTFSLVNLKITHGYASFDSGGAIVS"
BASE COUNT         1061 a         1267 c         1318 g         1109 t
ORIGIN      
        1 gtgcgtgcat ggaactggac gggaggccaa gccacgccgt gaatgtattc cagacaatcg
       61 cggcgattaa cgcggcgata atcactggcg gcgaagcgat cgccggcagg ataaagtcgc
      121 ggccgatggt ggtcgcgaca ctcaagccga agataaatgg cccgatgcca gccgaggccg
      181 cggcgatgaa gagcgcacgg cgcgggccga tagcgcgcga tgagatcatc gtggcgacga
      241 tattggcgga gtcgcggaag ccgctcaaga agccgaagag ggcggcgccg attaaaatgc
      301 tcagcgccac cggaccgatc gggttaagca tgaggccgaa gtttccgatc gaaccgcaga
      361 attaacggcg ggcagatgtg atcgatcaaa ataaaatatc ccgatgaaga atcatcggga
      421 tatagcatac catgctggct gaaatcgatg aagcgccact ctcagcgggc acgcgtgcgt
      481 atttctttca ggtaattctg ccagccggat acatcttcat tttgcttcaa cgcaaagtaa
      541 taatcgcgca cgatgtcacc ctgctgttcg cgattgaagt cgctgaactt ctggcctttc
      601 tcgctgagct ctttaaccgc atcgctcggg cgttgaccgg cggcttcgta cacgtaggtc
      661 ggcgcaaaag cggcttgaaa gagatattga atcccaacgt gttgatactg ccattgatgc
      721 gtcagttcat gcatcagcca gcccatatcg gtgagatcgc cggtgagctt gcgcggaaag
      781 tatgagacat tgccgacggt gatggcatta gcggcgggtg gcggcagccg cttgagccac
      841 gcgccgatgc ggcctacaat attgccgata gaactttctt ctttgatccg cacgcgctgg
      901 aggtctagtc cgtcgccgaa aagagcatta acttcggcta actcgatcgg gttgagtgaa
      961 cgcacagcca tattctttct ccctaaagca gaaatatgaa accagcagag gatcaggccg
     1021 gagcgtcctg atacgctttg tgactggggc aagcagctcc ggttgtgaat atcgcggagc
     1081 tgacgctccg cttcaagcat tatactttac tcgtcttctt cgtcgggatc aggttccggc
     1141 ttgacgttgt gcaggatgtc gcgcggatcg atgtccgcca cttggccgtt gtcgtattga
     1201 atcttgatgt gatctttgcc cacgccgatc acggtgccga cggcgcgccc gctgcgcagc
     1261 aaaaatcgat ccccaacttt ccattcatcc ggcatgctct gcctccgcat ttattatagc
     1321 attctgctgc aactgtgttg cgcatgatcg agtttttttc ttcagtatgc tgtgcctctg
     1381 gcaaacctct gggaggaagg ctcgctggtc atccctcttt cgcgttttat tgagtttcgc
     1441 ataaagaatg caaagtttga ttagctgcac tatcctgcgt ggttatgcga cctacgggaa
     1501 cagcacagag cagcagtggt gtgcatcgca ggcggcttca tcactgcggc ggtctcgttg
     1561 atcggcttgt tgcatcccaa agtcaaaaga ctggattgat tttatttcga tcgctgacac
     1621 acacacgctt tgataacggg cgttttttgt ttgtgtggta gactcgatcg atgttctccc
     1681 ctcgatcgta tttaaaaaaa cttcacatga cgaggggtag aatcaattta tcgaaagcag
     1741 ttatacacag gaggcaaaaa tgcgacgatg gcagactgta tggagcgtaa tactcttggg
     1801 cttagccagt gtggcgagtc tcatgctaac cttaggcgtg tccggagcac gtccagccgg
     1861 tgcggcaaga tcgatcgctg gagtcgatga tcaaagcggt cccacgcttg tctccagcac
     1921 aggaaaaatc gcttatgtat ttcagagcga caccgcgccg aacgatcagg cgaatatggc
     1981 ttttctaaac agtcgcggct ataccgtcac gcctgtgctg ttgagtttcg tgtccagcac
     2041 cgacttcagc cccttcgatc tgatcatgat cggcgaaggc actggtacct tgaatgagtg
     2101 gggcactgtg gcggccgatg tgccagccat cattgcgccc aacaaaccga tcatgggctt
     2161 gagcgaaggc ggctatgctt tcttcggcaa actctctttg tttatcggct ggccgcgagg
     2221 ctggcactca acggatctgg taatgacacg cgtgctgact gcgccgaatg cgatgtttac
     2281 cacgcccaat ctcgtcagcg gcagtccgat cacggcgtat acgcaaaata tcaccgaagt
     2341 cgccatttac ctggcggcta aaccggccga cgtggtggcc ttcgccgaag aaacgccatc
     2401 gaccaatcac gccatgctta tcagtcaggg ctgccgatat ttgtggggct ttggcggcag
     2461 tccgttgatc atgaccgctg ccggtcagaa tctattccag aacaccgtcg aatacgcgct
     2521 gcattctaat tgtccaccgc cgccgccgct gccgaccggg gagtgtgtca ccatcagcaa
     2581 ggcttcagcg ccggccggta atacaccggt aatgccgggc gacaccattc actacacgtt
     2641 gaccttgctg ctaaaaaaca ttttatcttg tcaaaaccaa acggggctgg tcatcgatcg
     2701 agttccaacc gatacattgg ttattagcaa cagccttggc caaggtggtt acttagctca
     2761 agacaatgcg gtggtctggc aggtatcacc ggcggcggcc ccctttaccc gcaccttcag
     2821 tgttaacgta tcgctgctgt cgtgtttgca cacaccgctg gtgatcaatg ccgcaacgct
     2881 gggtttaacc aacaccgtct cagttaagag caatgtcgtg cagcatcccg ttatttgtcc
     2941 gacgacgatc tttaacattt atctgccgct gagcctgaag tgaggtgtgg aggctggcgg
     3001 tcacctagct gatttgtttg tttcatatgt tgcctgagca tagaataaac ttgtgcagag
     3061 aaaatctgat tggccagtca aacctgatcg cgatcatatt aaaatactga gcgcacagtc
     3121 gaaaattgca gcaggtgtcg tcttgaacaa acgctttcta cttttgttga gcagcgtggt
     3181 gatcatgttc atcatgatct ggatgttcat cggcagccaa ccggccgttt cggcttcaac
     3241 gcccagtcgc cgttcgagta ggcttgatac ggtaaccagg ctcaccgatc gagcgttgct
     3301 gacttcaacg cttgctttca ccgcctatct gccgctcgtc attaaacaac ctgccgcacc
     3361 gccggcaacg aattggctgg actacgtcaa ttattatcgc gcacttgcga acctgccgag
     3421 cgtgaccgag aatccgatct ggagcgatgg cgattggaaa cacgggcgct atatggtgaa
     3481 gaacgatttc atcggccatg acgaagatcc ggccaatcca tggtatacac ccgaaggccg
     3541 cactgccgcc cagaatggga acaccatggt gagcagcagc gcgaccgtaa ctgattccta
     3601 cgcaatcgat ttctggatgc gcgcaccctt ccacgcggtg ggcatcctcg atccagcctt
     3661 ggggcagata ggctttggca gttatcgcga ggcggatggc ggctggcaga tgggcgcaac
     3721 gctcgatgtg attcgcggcc tgggtgccat tccgccagca gttgcatttc cgatcgagta
     3781 tccaggtgac ggcgcgacga tcgggctgcg ttcatatacg ggtggtgagt ggcctgatcc
     3841 actcagtagt tgcgcgggct ataccacgcc gagcggcctg ccgatcattt tgcaaattgg
     3901 cccgggcaat ctgatgccca gtgtcacgac tcattcgttc aagcaaagca ccacatcatt
     3961 agaacactgt atcttcgatg agacgaacta cgtcaatccg gatagcacca ctcaaggcgt
     4021 tgggcgcagt gtgctcaatg cgcgtgatgc catcgtgttg atcccccgtt cgccattgac
     4081 cgccggtgcc acctacaccg tgtcgatcgc gaccaatggg cagacttaca cctgggcatt
     4141 tactgtggcc agtacagcga ctgtgaacga attcgctgga ccgctgtcga tgcgttgagg
     4201 ctcaagcact ggcctctcga tgcttgccct aaagagctgg gcagttggtg cggctgcctg
     4261 atacgaacaa tcaaatgcgg ctgtttataa gagggatcga gtttatctca acagcgagga
     4321 tgtgatagga tcatcagcaa gcaattggaa caggagggtg cagtatgttt cgaacaatcg
     4381 aacgtctagc ccttgtcttc atcgccgcga tcataattgt gttgattgcg atcggcattg
     4441 tgcgcggcgc gccacaaagc ggagtcgtcg gtaccggcac agcgctgagt tgtgatgaag
     4501 cggcgtttga tgcgaggctg gccgggggcg gattgatcac cttcaattgc ggagctgctc
     4561 cagtcacgat tactctaagc agcgaaattc caatcaccgc cactacggtc atcagtggca
     4621 atggtttgat cacgttaagc gggaacgatg ccacgcggct gttcaatgtg ggatcgggcg
     4681 taacattcag cttggtgaat ttgaaaatta cccacggcta cgcgagcttc gattccggcg
     4741 gcgcgatcgt gtcgc
//
LOCUS       DASRTJ010000628         8613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_361015,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000628 DASRTJ010000000
VERSION     DASRTJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361015"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFF70_12815"
     CDS             <1..203
                     /locus_tag="VFF70_12815"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZY45619.1"
                     /translation="EMPADEADTLGGFVYSQLGKVPLPGDEVHYKNIMLKVMNIAGRR
                     IGKIRVTKRPEERIVDEEPSEE"
     gene            328..780
                     /gene="cdd"
                     /locus_tag="VFF70_12820"
     CDS             328..780
                     /gene="cdd"
                     /locus_tag="VFF70_12820"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZY45620.1"
                     /translation="MTNGSFSAREKVAHEVSTRKECSMNRGELIALAQKVRLRAYAPY
                     SHYKVGAALLGKSGRVYLGCNVENASYGLTCCAERNAVFTAVSAGETEFEAIAVATRN
                     SGSPCGACRQVLSEFAPDLKIYIASKDGRINTTSIKKLLPGAFSPNDL"
     gene            864..1655
                     /gene="recO"
                     /locus_tag="VFF70_12825"
     CDS             864..1655
                     /gene="recO"
                     /locus_tag="VFF70_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZY45621.1"
                     /translation="MPDRSRLYRIDAIVMHRSDMGEADRLLTVLSRDQGKLRVNAKGA
                     RKVASRKGGHVELFTRSKMLIAKSRGNIDIVSQVEMTEAYRGLHEDLTRSTYAHYAVE
                     LIDAFAEEGTAQPDIFDLLADALSWIANSNNLPLTTRYFELKLLTLAGFQPQVFVCAV
                     KGEPLEEIKVDENYGWSPNAGGAICPTHSEGRSDAGRLSLNALKVLRHALRSNYQTFT
                     ALTVSESVQNEIEQAMLRYVQYVLERHVKSIEFLNLLKRESANEP"
     gene            1752..4754
                     /gene="glyS"
                     /locus_tag="VFF70_12830"
     CDS             1752..4754
                     /gene="glyS"
                     /locus_tag="VFF70_12830"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZY45622.1"
                     /translation="MPKPLSFQQIILRLQEYWTSRGCSLWQPYNTQVGAGTMNPATFL
                     RVLGSEPWNVAYVEPSIRPDDGRYGDNPNRLQQHTQFQVILKPDPGNPQEIYLESLFA
                     LGIKRAEHDIRFVEDNWESPALGAWGLGWEVWLDGLEITQFTYFQQAGGITLDPVSVE
                     ITYGLERIAMFLQGVRSAYDIDYDGVHTYGDMFLRSEIEYCIYNYELADVDRLHQLYD
                     TYEAEAKAALTHEPPLVIPAHDYVLKSSHTFNLLDARGAIGVTERANYFRRMRDLSRA
                     VAQAYLKQREAMEFPWLKNDRGQQTPDQSKSKTASDQLSVIREPTTFVLEIGTEELPA
                     PDLTSALDQVREIAAKLLGEARLVYKNIWFFATPRRISFLIDELAPKQTAIDLTVRGP
                     AVKAAFDKDGKPTKAAEGFARGQGVPVTDLIKGEEKGAEYVFIKKHDAGKSTMEVLSS
                     LLPEMIAAIKFDKVMRWNNSGVSFSRPIRWLVALFGNQIVSFQYAGIHSGNVTRGPRA
                     AGAPEIKIESADQYLAILKQANVIAEIDERRTRIQAATTELAKSVKGSVPDDVSLLAE
                     VTNLVEQPTALLGSFDKKYLELPKDVLITVMKKHQRYFPLVGSADLSRLLPNFITIRN
                     GDDQHLDSVQHGNEEVIRARFADAEYFFNHDRQHQLEDFLTRLGTLTFHVKLGSMLDK
                     AKRIEALTPTIGSMLGLKESDLKFAQRAAHLSKADLATKLVVEMTALQGILGREYAKL
                     SGEPNEVANAIGEQYDRAPASKIGAAISLADRFDSLAGLFAIGLAPTGSADPWALRRA
                     ALNIIETLIAHQVSFSLTSALEAAAQLQPLAVESKSIDEAHAFIIGREKAAFLDQSLR
                     YDLVEAVLSARGDDPFSALQSIQQLSLWVAKPDWPQLLDGYARCVRIVRDITESLPLD
                     IKSDNDQNAQTLYQAYEAAQKSIGPASSIEDFLTALKKMIEPITNFFDKVLVMHDDPS
                     IRETRQALLLRIWHLADGIVDLTKVEGF"
     gene            4881..7670
                     /gene="ppdK"
                     /locus_tag="VFF70_12835"
     CDS             4881..7670
                     /gene="ppdK"
                     /locus_tag="VFF70_12835"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZY45623.1"
                     /translation="MSQKWVYLFSEGNKDMRDLLGGKGAGVAEMTRANLPVPPGFTIT
                     TEACNEYFKIGKKFPDGMWDQAMAALKSVEDQTGKHFGDATNPLLVSVRSGAKFSMPG
                     MMDTVLNVGLNPATLEGVAKLTHNDRFAWDAYRRLIQMFGKTVKGIEGHKFEAILTKY
                     KAKTKGKQDTDLTTDMLKDVVTDFKAVYKKELGVDFPDQVHEQLGQAIEAVFSSWFGK
                     RAVDYRNYNKISHDLGTAVNVQMMVFGNMGNDSGTGVAFTRNPSTGDQALYGEFLINA
                     QGEDVVAGIRTPKKIAQLKDEMPKVYDQFLKTGAMLEKHYREVQDLEFTVERGKLWML
                     QTRTGKRTAQAAIKIAVDMVKENLISKEEALQRIEPMQINQLLLPRFDEEAKKKAGVL
                     AKGLNASPGAATGLAVFDADTAEEWGKAGKAVILIRPETNPDDVHGMLVAKGILTQRG
                     GATSHAAVVARGLGKPCVAGTEGIRVDAEKKLFVADGKTIKQGEYISIDGSSGEVFAG
                     QIKTIDPDFSQEKDLIELLAWADQVRRLGVWANADYPKDAAKAREFGAEGIGLCRTEH
                     MFFEQIRLPFVQQMILNASEAQSKLDALARTEADLSKRPDNKETQKAHAAAKKVVTSS
                     SAVKLYKTALTKLLPMQRKDFEGIFKVMDGLPVIIRLIDPPLHEFLPSHDELLVTVTE
                     MRVLGKKGKKLKDAETMLAAVESMREANPMLGLRGIRLGITYPDILKMQVRAIFEAAC
                     SMQSQGVTVKPEVMIPLTGHFNELAVSQGALEEVAKQVMQEKGVDVEFKFGTMIEVPR
                     AALTAAEIAKYAQFFSFGTNDLTQTTYGYSRDDAEGKFLLRYVEDKILPANPFQQLDQ
                     IGVGQLIEMAVKNGRAARPGMEVGICGEHGGDPSSIEFCHRAGLNYVSCSPFRVPIAR
                     LAAAQAALGSTTRDK"
     gene            7819..8325
                     /locus_tag="VFF70_12840"
     CDS             7819..8325
                     /locus_tag="VFF70_12840"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZY45624.1"
                     /translation="MSQIIILEKSIAINATPAKIYNALINAQELRRWRADRAESDASI
                     GGTVVIVRGENEARGVYKRLMLNREVAIHWDRHDKLLPEDLTVFRLEKTAKGTKVIVN
                     NFALPVEVDRIGAEWDKQLKQLKKVYQAASPKPKAKSKAAKKTTAKRKSIAKIKSKSN
                     SSKAKWKK"
BASE COUNT         2207 a         2258 c         2323 g         1825 t
ORIGIN      
        1 gtgaaatgcc ggccgacgaa gccgatacgc tgggcggttt cgtctacagt caactcggca
       61 aagtgccgct gcccggcgat gaagtccatt ataaaaatat catgctgaaa gtaatgaaca
      121 ttgccggtcg acggattggc aaaatccgcg tgacgaaacg gccggaagaa cggatagtcg
      181 acgaagagcc gagtgaagag taatgcgtgc tcatgcgatg tgcaactttc tgaatcgacc
      241 acaatagaac cgtcattgcg agaggagcga agccatctcg ccaacaatca gctgagattg
      301 cttcggcgca ataagacgcg cctcgcaatg acgaatggat ctttttccgc ccgagaaaaa
      361 gttgcgcatg aagtcagcac gcgtaaggag tgttcaatga atcgcggcga attgattgcc
      421 ttagcccaaa aggtccgcct gagagcgtat gcgccctact cgcattacaa agtcggcgcg
      481 gcattgctgg gcaaaagcgg cagagtctat ttgggctgca acgttgagaa cgcgtcatat
      541 ggtttgacgt gctgcgcaga gcgcaacgcg gtcttcaccg cggtcagcgc cggcgaaaca
      601 gaatttgaag cgatcgcggt agcaacccgc aacagcggat cgccctgcgg cgcgtgccgc
      661 caagtattga gcgagttcgc gcccgatctg aaaatttaca ttgccagcaa agatggccgc
      721 attaacacga cctcgatcaa aaaattgcta cccggcgcat tttcacccaa tgatctctga
      781 tttctgattt gtcctatcca tcaacagttt gtcatttgtt atttggcatt cgtcattgtt
      841 aattattcat tggatattga aaattgcccg atcgatctcg cctctatcga attgacgcca
      901 ttgtgatgca ccgatcggat atgggcgaag ccgatcggtt gttgaccgtc ctatcgcgcg
      961 atcagggcaa gctgcgcgtc aacgccaaag gcgcgcgcaa agtcgccagc cgcaaaggcg
     1021 gccacgtcga gttgttcact cgatcgaaga tgctgatcgc caaaagccgt ggcaacatcg
     1081 acattgtgtc gcaagttgag atgactgaag cctaccgcgg tctgcatgaa gacttgaccc
     1141 gatcgacgta tgcgcattac gcggtggagt tgatcgatgc gtttgccgaa gagggcacgg
     1201 cgcaacccga tatttttgat ctcttagcgg atgccttgag ctggatcgcc aactcgaata
     1261 acctgccttt aaccacgcgc tatttcgagt taaagctgct caccctcgcc ggttttcaac
     1321 cgcaagtatt cgtatgtgcc gttaaaggtg aaccgctgga agaaatcaag gtggatgaga
     1381 attacggctg gagtccaaat gccggcggcg cgatctgccc aacgcactct gaaggtcgat
     1441 cggatgcggg tcgcttgtcg ctcaacgcgc tgaaagtttt acgtcacgcg ctgcgtagca
     1501 actatcaaac gttcaccgcc ttgaccgtga gcgaatcagt gcaaaacgag atcgagcaag
     1561 ccatgttaag atatgtgcag tacgttctgg aacgccacgt caaatcgatc gagtttctaa
     1621 atttactcaa gcgcgaaagt gctaacgaac cctaaagggc atgccagtaa gcaacgaatc
     1681 aacgaataaa atgaattact cgttactgat gactaatcac taattactct tcaatcttca
     1741 ctaggacact gatgcccaaa ccgttatctt ttcagcaaat tattttgagg ttgcaagaat
     1801 actggaccag ccgcggctgt tcactgtggc agccctataa cacgcaggtc ggcgcgggca
     1861 cgatgaatcc cgccacgttc ctgcgcgtgc tcggctccga accgtggaat gtcgcatacg
     1921 tcgaaccctc gatccggccg gatgatggcc gctacggcga caatcccaat cggttgcagc
     1981 agcatacgca atttcaagtc atcctcaaac ccgatccggg caatccgcaa gaaatttatt
     2041 tggaatcgct attcgcgctc ggcatcaaac gcgctgagca cgatatccga ttcgtcgaag
     2101 acaattggga atcaccggcg ctcggcgcgt ggggacttgg ttgggaagtg tggctcgatg
     2161 gcttggagat cacgcagttc acatactttc aacaagcggg cggcatcacg ctcgatccgg
     2221 tctccgttga gatcacctac ggtctcgaac gcatcgccat gtttctgcaa ggcgtgcgca
     2281 gcgcgtacga catcgattac gatggcgtgc atacttacgg cgatatgttt ttgcgctcag
     2341 agatcgaata ttgcatctac aactacgaac tcgcggatgt cgatcggctt caccagctct
     2401 acgatactta cgaagccgaa gcgaaggccg cgctgacgca tgaacctccg ctggttattc
     2461 cggcgcacga ttatgtgctc aagtcctcac acaccttcaa tctgctcgat gcgcgcggcg
     2521 caatcggtgt gaccgaacgc gcgaattatt tccggcggat gcgcgatcta tctcgagcgg
     2581 tggcacaggc ttatttgaag cagcgtgagg cgatggagtt tccgtggtta aagaacgatc
     2641 gcggacagca gacgccagac caaagcaaat caaaaacggc tagcgatcag ctttcagtga
     2701 ttagggagcc tacaactttt gtacttgaaa ttggaacaga ggaactgcct gcacccgatt
     2761 taaccagtgc gctcgatcaa gtgcgcgaga ttgcagccaa attgttgggc gaagcccgat
     2821 tggtttacaa aaacatctgg ttttttgcta ctccgcggcg aatttcgttc ctcattgacg
     2881 aactcgcacc gaaacaaacg gcgatcgatc ttacggttcg cggaccagct gtcaaagctg
     2941 cgttcgataa agatggcaaa ccgactaaag ccgctgaagg tttcgcgcgc ggtcaaggtg
     3001 tgccggtaac tgatttgatt aaaggcgaag aaaaaggcgc ggagtatgtc tttatcaaga
     3061 agcatgatgc aggcaagtcg accatggaag tgctttcgtc tctgctgcca gaaatgatcg
     3121 ccgctatcaa atttgacaag gttatgcgct ggaacaacag cggcgtttca ttctctcgac
     3181 cgattcgctg gctggtagca ttattcggca atcaaattgt gtctttccaa tatgctggca
     3241 tacacagcgg caacgtcacg cgcggaccgc gtgccgctgg agcgcctgag atcaagattg
     3301 aatctgccga tcaatatctc gccatattga agcaagccaa cgtcattgct gagatcgacg
     3361 agcggcgaac tcgcatccaa gccgctacga ctgaactggc caagagtgtg aaaggctcag
     3421 tgccggatga tgtttcacta ctcgctgagg tcaccaatct ggtcgagcag cccactgccc
     3481 tgctcggctc gtttgacaaa aaatatcttg aactgccgaa agatgtgctc atcacggtga
     3541 tgaagaagca tcaaagatat tttccgttgg tcgggagcgc cgacttgagt cggctactac
     3601 caaacttcat caccatccgc aatggcgacg atcaacatct cgacagtgtg cagcatggca
     3661 acgaggaagt gattcgggcg cgtttcgccg atgccgaata tttcttcaat catgatcgac
     3721 aacaccagct ggaagatttt ctgacgcgac ttggcacgct gaccttccac gtcaaactcg
     3781 gatcgatgct ggacaaagcg aagcggatcg aagcgttgac gccgacgatt ggctcgatgt
     3841 taggcctgaa ggagagcgat ctcaaattcg cgcaacgcgc cgcgcattta agcaaagccg
     3901 atctcgccac aaagcttgtc gtcgagatga cagcgctgca aggcatcctg ggccgcgagt
     3961 atgcaaagtt aagcggcgaa ccaaatgaag tcgcaaatgc gatcggtgaa caatatgatc
     4021 gcgcgcccgc ctcgaagatc ggtgcggcga tcagcctggc cgatcgattt gatagcttag
     4081 ccggcttgtt tgcgatcggc ctggcgccga caggctcggc tgatccgtgg gcgctgcgcc
     4141 gcgcggcctt gaatatcatc gagacactca tcgcgcatca agtttctttt tcactgacga
     4201 gcgcgcttga agcagcagcc cagctgcaac cactcgccgt cgaatcaaaa tcgattgacg
     4261 aagcccacgc cttcatcatt gggcgtgaaa aagccgcatt cctcgatcaa agcctgcgct
     4321 acgatctggt cgaggcggta ttgtctgcgc gcggcgatga tccattcagt gcattacaat
     4381 cgatccaaca actgagcctg tgggtggcca aacccgactg gccgcagctg ctcgacggtt
     4441 atgcgcgctg cgtgcggatt gtgcgcgaca ttactgagtc gctgccactc gacatcaagt
     4501 cggataacga tcaaaatgcc caaacgctgt atcaagcata cgaagccgcg cagaaatcga
     4561 tcgggccggc tagctcgatc gaggatttct taactgcctt gaaaaagatg atcgaaccga
     4621 tcactaattt cttcgacaaa gttctggtca tgcacgacga tccatcgatc cgagaaacgc
     4681 ggcaagcctt gctactacgg atatggcatt tggcggacgg catcgtcgat ctcacgaagg
     4741 tggaagggtt ttaagtttat tacacatcac atcaaactca tggttatttg tcactagccc
     4801 aaaccgtcta gcatcgatta gacttaatcg attggaaatt catcaacatc gcaattatta
     4861 aacacgttaa ggagaacaag atgtcacaga agtgggtgta cctatttagt gaaggcaaca
     4921 aagacatgcg cgatctgctc ggcggcaaag gcgccggcgt ggccgagatg acacgcgcga
     4981 atttgcccgt gccgcccggt ttcacgatta caaccgaggc ctgcaatgag tatttcaaga
     5041 tcggtaagaa gttcccggac ggaatgtggg atcaagcgat ggccgcgctg aagtcggtgg
     5101 aagatcaaac cggcaaacac ttcggcgatg caaccaatcc cctgctcgtc tcggttcgat
     5161 ccggcgcgaa gttctcgatg cccggcatga tggataccgt cttgaacgtc ggcctgaatc
     5221 cagcgacgct agaaggcgtg gcgaagttga cccacaacga tcgctttgcg tgggatgcgt
     5281 atcgacgctt gatccaaatg tttggcaaga cggtcaaagg cattgagggt cacaagttcg
     5341 aagcaatcct caccaagtac aaagccaaga ccaagggcaa gcaggatact gatctgacga
     5401 ccgacatgtt gaaagacgtc gtcacagatt tcaaagccgt gtacaagaaa gaactcggcg
     5461 tcgattttcc ggatcaggtt catgagcagc tcggtcaggc gatcgaagca gtattctcgt
     5521 catggttcgg caaacgcgcc gtcgactatc gcaactacaa caaaatttcg cacgacctcg
     5581 gcacagccgt caatgttcag atgatggtct tcggtaatat gggcaacgac tccggcaccg
     5641 gcgtcgcctt cacacgcaat ccatcgaccg gcgaccaggc cttatatggc gagttcttga
     5701 tcaatgcgca gggcgaagac gtggtcgccg gcattcgcac gccgaagaag atcgcgcagt
     5761 tgaaggacga aatgcccaag gtgtacgatc aattcctgaa gaccggcgcg atgctggaga
     5821 agcactatcg tgaagtgcag gacttggaat tcaccgtcga gcgcggaaaa ttatggatgc
     5881 tgcaaactcg tacgggcaaa cgcacggccc aggcagcgat caagatcgcc gtcgatatgg
     5941 taaaagaaaa tttgatcagc aaagaggaag cactgcagcg catcgaaccg atgcagatca
     6001 atcagttgtt gctgccacgc tttgatgagg aagcgaagaa gaaagccggc gtgctggcca
     6061 aaggtctcaa cgcatcaccc ggcgcagcca ccggcctcgc agtcttcgat gcggacaccg
     6121 ctgaagaatg gggcaaagcc ggcaaggccg tgatcctgat tcgacccgaa acgaatccgg
     6181 atgatgtgca cggcatgtta gtcgccaaag gcattctcac tcagcgcggc ggcgccacct
     6241 cgcatgcggc ggttgtggcg cgtggactcg gcaagccgtg cgtcgcgggc actgaaggca
     6301 ttcgcgtcga tgcggaaaag aaattgttcg tcgccgatgg caagaccatc aagcagggtg
     6361 aatatatttc gatcgatggc agcagcggtg aagtgttcgc cggacagatc aaaacgatcg
     6421 atccggattt cagccaagaa aaagatttga tcgaactact agcttgggct gatcaagtcc
     6481 gccgattagg tgtctgggca aatgcagatt atcctaagga cgcagccaag gcgcgtgagt
     6541 tcggcgcaga gggcattggg ctgtgccgca cggagcacat gttctttgaa caaattcgct
     6601 tgccgtttgt ccaacaaatg attctcaatg ccagcgaagc ccaatcgaag ctggatgcac
     6661 tggcgcgcac tgaagccgat ctatccaagc ggcctgataa caaggaaacg cagaaagcac
     6721 acgccgcggc caagaaggtt gtgacttcat cgagcgccgt gaaactctac aaaacggcac
     6781 tcactaagtt gctgccgatg cagcgcaaag acttcgaagg catcttcaaa gtcatggacg
     6841 gtctgccggt catcatccgt ttgatcgatc ccccgctgca tgaattcctg ccgtcgcatg
     6901 atgagttgct cgtcacggta actgaaatgc gcgtgctcgg caagaagggc aagaagttga
     6961 aagacgcgga gacgatgttg gccgccgtcg aatcaatgcg cgaagcgaac cccatgctcg
     7021 gtctgcgcgg catccgtctg ggcatcacct atcccgatat tcttaagatg caagtccgcg
     7081 cgatcttcga agccgcctgc agcatgcaat ctcaaggcgt aacagtgaaa cccgaagtga
     7141 tgattccgct caccggccac ttcaacgaac tcgccgtgtc gcaaggcgcg ctcgaggaag
     7201 ttgcgaagca ggtcatgcaa gaaaaaggcg tcgacgtcga attcaagttc ggcacgatga
     7261 tcgaagtgcc gcgcgccgcg ttgacagccg ccgagatcgc caagtacgcg cagttcttct
     7321 cattcggcac gaacgatctc acccagacca catatggtta ctcgcgcgat gatgccgaag
     7381 gcaaattctt gctgcgctat gttgaagaca agattctgcc cgccaatccg ttccagcagc
     7441 ttgatcaaat tggcgtcggc caattgatcg agatggcggt gaagaatggc cgcgccgctc
     7501 gacccggcat ggaagtgggc atctgcggcg aacacggcgg tgatccatca tcaatcgaat
     7561 tctgccaccg cgccggtttg aactacgtga gctgttcacc gttccgcgtg ccgatcgcgc
     7621 ggctagccgc ggcacaagct gcactgggat cgaccactcg agataagtaa gacaagttgc
     7681 caacccttca tcttcgctca gggcaggctt gtcctatgca caaaactttt caaaccgccg
     7741 atcgcactcg atcggcggtt tggcttgtct gtccaattga tcgtataatc taagtagcat
     7801 ttaagcagga ggttggtcat gtcacaaatc atcatcctcg aaaaatcgat cgcgatcaat
     7861 gcgacacccg ccaaaatata caatgctctg atcaacgccc aggagctcag gcgctggcga
     7921 gccgatcgcg ccgagtccga tgcgagcatc ggcggcacag tcgtgatcgt tcggggcgag
     7981 aatgaagcgc ggggcgtcta taaacgcctg atgcttaatc gagaagtcgc tatccattgg
     8041 gatcgacacg ataaattact gccggaggac ctgacagttt tccgcttgga gaaaacggcg
     8101 aagggcacca aggtgatcgt caacaatttt gcgctgccgg tagaagtcga tcgcatcggc
     8161 gccgagtggg ataaacagct caaacaattg aagaaggtgt atcaggctgc ctcgccaaaa
     8221 ccaaaagcaa aatcgaaggc cgccaagaaa acgactgcaa aacgaaagtc aatagcgaaa
     8281 ataaagtcga agagcaacag cagcaaagca aaatggaaga agtaacttct aggcggctaa
     8341 ttacgattat cgatcatttg tcattggtcc ttgttaattg ttcatggctt tggtgcatga
     8401 atcgaacaac aggcgtgttt gatcacagcc attgatcggt taagctactt ggtaactgtc
     8461 tggcatgccc aagtgagtca ttttgttcag agccatgcac cggattaagg cttgagttgt
     8521 ttgggtttca agcaagcgtg tcgagaggcc agcgccaaaa atggttttta ggcgaaacat
     8581 gatcgtctcg gccaaagaac ggacatgata acc
//
LOCUS       DASRTJ010000629         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_208516,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000629 DASRTJ010000000
VERSION     DASRTJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208516"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1671
                     /gene="pepF"
                     /locus_tag="VFF70_12845"
     CDS             24..>1671
                     /gene="pepF"
                     /locus_tag="VFF70_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HZY45625.1"
                     /translation="MSNTQIPARDQIAHEYTWNAESVFASPQAWQAEWNSIASDLPNL
                     KKFQGHLADGASMLADALDAIDQIVRRIGKVYVYASMSHAADTQNQAASKMNSEAQGL
                     FGQVSGAVSFLDPELIAIGEAKLRQWLKAEPRLAHIGHYIDNLFRKQAHVRSGEVEEL
                     LGMLSDPFSGISTAEGALTNADFKFAPAIDSAGKELPVTQSSVYDIMHQPDRAARRSA
                     RENYRDSYLAHKNTVAANLSTSIKQNVFMMRARQQPSTLEATLFNTNIPVEVFHNLIN
                     TFRKNLPTWQRYWRLRRKALGVDRLESYDLWAPLTTAKPKVPYPQAVEWICAGLEPMG
                     QEYVDILRRGCLQDRWIDLLPNQGKSAGAFSTGWPGTHPFIMMSYADDVFSLSTLAHE
                     LGHSMHSYYTWKNQPTLYFDYSTFVAEVASNFHQAMVRDYLLKHNPDRDFQIALIEEA
                     LGNFLRYFFIMPTLARFELETHQRVERGEGLTADSMIDLMADLFSEGYGGEVHVDHDR
                     VGMTWATFGHLYADYYVFQYATGISGAQALAKRVLSGEKGA"
BASE COUNT          383 a          458 c          473 g          357 t
ORIGIN      
        1 gtcatgacct taaaaggctg aacatgagca atactcaaat tcctgcccgc gatcaaatcg
       61 cgcacgaata tacctggaac gccgaaagtg ttttcgcttc gccgcaagcc tggcaagccg
      121 agtggaacag catcgccagc gatctgccga atctcaaaaa atttcaaggt catcttgccg
      181 acggcgcatc gatgttagct gatgcgctgg acgcgatcga tcagatcgtc cgccgcatcg
      241 gcaaggtcta cgtgtacgcc agcatgtcgc acgcggccga tactcagaat caagcggcgt
      301 cgaaaatgaa cagcgaagcg caaggtttgt tcgggcaggt gtccggcgcc gtctcgtttc
      361 tcgatccgga attgatcgcg atcggcgaag cgaaactgcg gcagtggctg aaggccgagc
      421 cgcgcttggc gcacatcgga cattacatcg acaatttgtt ccgcaagcag gcccacgttc
      481 gatcgggcga agtggaagaa ctgttgggca tgctgtccga tccgttttca ggcatatcga
      541 ccgccgaagg tgcgctgacg aatgccgatt tcaaattcgc gcctgcgatc gactcggcag
      601 gcaaagaact gcccgtcacc caaagcagcg tctacgatat catgcatcag cccgatcggg
      661 ccgcacgccg atcggcccgg gagaactatc gggattcata tctggcccac aagaataccg
      721 tcgccgcgaa cttgtccaca tcgatcaagc aaaatgtttt tatgatgcgc gcgcggcagc
      781 agccttctac attagaagca accttattca acaccaacat cccggtcgaa gtcttccaca
      841 atttgatcaa taccttccga aaaaatttgc cgacgtggca gcgctattgg cgcttgcgcc
      901 gcaaagcgct cggcgttgat cgattggagt cgtatgatct gtgggcgccg ctgacgacgg
      961 cgaaaccgaa ggtgccgtat ccacaagcgg tcgagtggat ctgcgcagga cttgagccga
     1021 tgggtcaaga gtatgtcgat attttgcggc gcggttgtct gcaagaccgc tggatcgatt
     1081 tgctgcccaa tcaaggcaag agcgcgggcg ccttctcgac gggctggccc ggcacgcatc
     1141 ccttcatcat gatgagctac gccgatgacg tgttcagcct gagcacgttg gcgcacgaac
     1201 tcggccattc gatgcattcg tattacacgt ggaaaaatca gccgactttg tatttcgatt
     1261 attcaacttt cgtggccgaa gtggccagca acttccatca agccatggtg cgcgattatt
     1321 tgttgaagca caatcccgat cgggatttcc agatcgcgct gatcgaagaa gcgctgggca
     1381 acttcctgcg ttacttcttc atcatgccga cgctggctcg cttcgaattg gaaacgcacc
     1441 aacgggtgga gcgcggcgaa ggcttgaccg cggacagtat gatcgatctc atggccgatc
     1501 tatttagcga agggtatggc ggtgaagtgc atgtcgatca cgatcgcgtc ggcatgacgt
     1561 gggcgacctt cggacatttg tatgccgatt actatgtgtt ccaatatgcc acggggattt
     1621 ccggcgcgca ggcgctggcg aaaagagtgt tgtccggcga aaaaggcgcg g
//
LOCUS       DASRTJ010000630         5945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_361111,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000630 DASRTJ010000000
VERSION     DASRTJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361111"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFF70_12850"
     CDS             complement(<1..229)
                     /locus_tag="VFF70_12850"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZY45626.1"
                     /translation="MDSITLLLDQMAYGGEAIGRYAGQAIFVQGGIAGETVRVEIVEQ
                     HDRFSRARLIEVLEPSAQRVEPRCPHFGFDPN"
     gene            398..1714
                     /locus_tag="VFF70_12855"
     CDS             398..1714
                     /locus_tag="VFF70_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45627.1"
                     /translation="MTSLLKSLTENKKLLIYAILGAMVIVSVLKSNYFDAIWTIITFS
                     LAYILMAWLLAPMLLPIDRSGEERGRLRDWFLRFLSGEKLAFIVVREGKVVAPAKKEK
                     RKPTKDKPEEEEGPWVGRGAILTDSTSVVVMKTETGISHVVGPGSAEEGKSESGVIFT
                     YYREQVDSIIDLRPQIRSHEMEAMTRDGIPVKVRVTAFFTLKGTKAKRSIDMAQSTAH
                     PFTWRQASVRQSFHNKRIANVEGKPEQMRQTQWSDRVLEIAVPQLRQLIAQSTLDYLT
                     APLLAERHPRFEIKKQLIPIVQRELDSQDDFKRGSGIQINNIAVSIMWPPPQVVEQRI
                     EAWKKEWHKKETEVMGRAEAQAMITKEQARANAQGELTARINDTLQNAKASGTSSSDL
                     VALRFLEVMEKMAKDPTTRALLTLDSLNMLNKLREMLKVRGSDDTP"
     gene            1711..3483
                     /locus_tag="VFF70_12860"
     CDS             1711..3483
                     /locus_tag="VFF70_12860"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HZY45628.1"
                     /translation="MSTNPTQHHGTLQDWIDLHPRPIGLLRLMITLGLLGLLSLVGTI
                     IGSTQVAAARDASGCTYQAEEANPPLSGQTAETGSAFGISWKIRNTGSCQIWGTGVAL
                     VRRADAIASDQAAYAIKTDQASGLNVLSVVTQMTAPDQPGVYETAWQMQAPNGQAFGP
                     VMTRRVQVYPAGQPQPNPPRNQDFINLTALFQLGVSLLLYPLPAAFAILLVLWRGADF
                     LQNVYQLKSLPMSHISGMMFGGNAGHLRVRSNKSESDSSTAAMEMIGGPIWLTVTDRT
                     AALTERGAKLARILGPGVHLLHAHERVRGVVDLQIQHRRQKQKTLSKDGIPLELDVDL
                     TFRVGERDIEGEAPPPPPPPIGPRARIRLLIGLPVSPMLLEASKPHRFSREAIRRIVY
                     ETPIASPDAPTDWTAGFTLMRTGELAKEFAERRLDDLSSPEDPDIHPLREIVEGGLTK
                     IRAEVAPSGIDILNMEIGIVEPPADIKGHVTEQRISNWMIEWKRRATILKSEGDAIAM
                     QAQEEAHAEAQANMIQALTEGFRIATAGNASVSSEVIAMRFIDALEALMHSQPEKQTA
                     NDREEEQPDQAAQTMLIVRQNQKE"
     gene            3490..4644
                     /locus_tag="VFF70_12865"
     CDS             3490..4644
                     /locus_tag="VFF70_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45629.1"
                     /translation="MPEHLSIANLLSLLEIDRQDDALQLCAEADSLCRRRRYDAAIEV
                     AERAAHLARNHYERLGVALLYLSSIRASAHQAGQAEQAQRDCERAVRALSTQPHNYVI
                     AQIIRGQIELEIDESANREAALDEFGMAHFSLQKLILDSREHNRAQHTALYRSLEKSL
                     TARIEHLSTALADVEPIEVAPKQPSADQSTPVPAFADQATIPTRPGQATPPPIKLAVP
                     TKLIWPSTELPSIEVVAAIGGATFDFIEVNQLSIEGRDYKVQPIVPVSSGSASLRLQH
                     GQQYMTLQIDGANDQRVLIRRQDRPDQRRQFIVVADSSQHQAWIDDAESTVPFKVVHI
                     IGAEREWNIRNGSDTSPINENELRIIGIVEAILTPAESTEPLIDLEQVAA"
     gene            4746..>5945
                     /locus_tag="VFF70_12870"
     CDS             4746..>5945
                     /locus_tag="VFF70_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZY45630.1"
                     /translation="MAEDPNSKLPPPQPIRLANGNINFVYRDLNGQFISEARYHEMKM
                     LLTNGHGDLSTPHKLRARLHRQLDESTALQDLKRDLAAHRTAHKRLLLNDADLSGLPL
                     THMDLHDADLINAKLMETNFTDANLRNSNLRGADLTQAILRRTDLAQSDLSSAELRFA
                     DLRETKLKDAQLIGTQADRANFSRSDLRSAVMSDKTSLQNVNLTEALLNHAQLAGVNF
                     SGAQLKGADLRWSKLAKANLSKAQLINARLEGVDLSGADLRGANLKGAQLRETNLHGS
                     LIDNDTLLDPEARRRWNAANLQNPKASTGRGSFTGAILHGAQFAGFDLRGSDFTRADL
                     AQANLHGAQLQGVSLIEANLESADLHNAQLERANLNSAYLGDANLKNANLRDADLTNA
                     DLRNAKLQ"
BASE COUNT         1401 a         1748 c         1639 g         1157 t
ORIGIN      
        1 agttaggatc gaagccgaaa tgtggacagc gtggctcgac ccgctgcgcc gatggttcga
       61 ggacttcgat caatcgggcg cgggaaaacc gatcgtgttg ttcgacaatc tccacgcgca
      121 cggtctcgcc ggcgatgccg ccctgcacaa agatcgcctg gcctgcgtag cggccaatcg
      181 cctcgccgcc ataggccatc tgatcgagta gcagcgtaat cgaatccata cctttattat
      241 actttctccg gtcaggttgc tgaatcgtgg ctttttcttt ttgcggcctt ttatgctata
      301 ttactctgcg acaacgcggc gcgcgagcat ccagagcgga acgaagtgaa gtcgagggac
      361 gcgcgccact ccgctcagag tgctaaacta agttcacatg accagcctgc tcaaatcgtt
      421 gacggaaaat aagaaactgc tcatctacgc catcctcggc gcaatggtga tcgtgtccgt
      481 attgaagagc aattacttcg acgcgatatg gacgatcatt acctttagtc tcgcttacat
      541 tctaatggca tggctgctgg ccccgatgct gctgcccatc gatcgatccg gcgaagagcg
      601 cggccggctg cgtgattggt tcctccgctt tttatccggc gaaaaattag cgtttatcgt
      661 ggtgcgggaa ggcaaggttg tggcaccggc caaaaaagag aagcgcaaac cgacgaagga
      721 caagcctgaa gaggaagagg ggccgtgggt cgggcgcggc gcaatcctga ccgacagcac
      781 cagcgtcgtc gtgatgaaaa ccgagaccgg catctcgcac gtggtcggac cgggcagcgc
      841 cgaagaaggc aagtccgagt cgggtgtcat cttcacctac tatcgggaac aggtcgattc
      901 gatcatcgat ctgcgtccac aaatccgcag ccacgagatg gaagcgatga cgcgtgatgg
      961 cattcccgtc aaggtgcggg tcaccgcgtt ctttactctg aaaggcacta aggccaagcg
     1021 ctcgatcgat atggcgcaga gcaccgcgca tcccttcacc tggcggcagg ccagcgtgcg
     1081 ccaatccttc cacaacaaac gcatcgccaa tgtcgaaggc aagccggaac agatgcggca
     1141 aacgcagtgg agcgatcggg ttttggagat tgcggtcccg cagctgcgcc agttaatcgc
     1201 ccaatccacg cttgattatt tgacagcccc gctgctggcc gaacggcatc cgcgcttcga
     1261 gatcaagaag cagctgatcc cgatcgtgca gcgcgagctc gactcgcaag atgacttcaa
     1321 gcgcggctcc ggcatccaga tcaataacat tgcagtcagc atcatgtggc cgccgccgca
     1381 agtcgtggaa cagcgcatcg aagcgtggaa gaaagagtgg cacaagaaag aaacggaagt
     1441 catgggccgc gccgaagcgc aggctatgat cacgaaagaa caggcgcgcg ccaacgcgca
     1501 gggtgaactg acggcccgca tcaatgatac attgcaaaat gccaaggcca gcggcaccag
     1561 cagcagcgat ctggtcgcgc tgcgcttttt ggaagtcatg gaaaaaatgg ctaaagaccc
     1621 caccacccgc gcgctgctga cactcgattc attgaatatg ctgaataagc tccgcgagat
     1681 gttaaaggtc cgcggatcgg acgatacgcc atgagcacga atccgacgca gcatcatggc
     1741 acgttacagg actggattga tctgcatccg cgtccgatcg gtttgctgcg cttgatgatc
     1801 actttaggtc tattaggcct cttgtcgctc gtcggcacga tcatcggttc gacccaggtt
     1861 gccgccgcac gcgacgccag tggctgcact tatcaggctg aagaagccaa tccgccgtta
     1921 tccgggcaaa ctgctgagac cggatcagcg ttcgggatta gctggaaaat ccgtaatacc
     1981 ggatcatgcc aaatctgggg cacgggtgtc gcgctggttc gtcgagctga tgccatcgcg
     2041 agtgatcaag ccgcctatgc gatcaagact gatcaggcca gtggcttgaa tgtattgagc
     2101 gtggtcaccc agatgacagc gccggatcag ccgggcgttt atgaaacggc gtggcagatg
     2161 caagcgccca acggccaggc ctttggcccg gtgatgactc gtcgggttca ggtttatccg
     2221 gccggtcagc ctcagccaaa tcccccccgc aatcaagact tcattaatct gactgcactg
     2281 tttcagctgg gcgtgtcatt attgctgtat ccattgcccg ctgcgtttgc catcctgctc
     2341 gtcttgtggc gcggcgcgga tttcctgcag aatgtgtatc agctgaaatc actgcccatg
     2401 agtcatattt ccggcatgat gtttggcggc aacgccggac acctgcgcgt ccgaagcaac
     2461 aagagcgaat cagattcatc gaccgcggcg atggaaatga tcggcggccc gatctggttg
     2521 acggttaccg atcgaaccgc agcgctgacc gaacgcggcg caaagctcgc gcggattctt
     2581 ggcccgggcg ttcacttgct gcacgcacac gagcgcgtac gcggcgtcgt cgatcttcag
     2641 attcaacatc ggcggcaaaa gcaaaagacc ttatccaaag acggcattcc gcttgaactc
     2701 gacgtcgacc tcactttccg ggtaggcgaa cgcgacattg aaggcgaagc cccgccgccg
     2761 ccgccgccgc cgatcgggcc gcgcgcacgc attcgtttat tgataggcct gcccgtctcg
     2821 cccatgctgc tggaagccag caaaccgcat cgcttctcgc gtgaagccat tcgccgcatt
     2881 gtgtatgaga cccccatcgc ttcacccgat gcgccaaccg attggaccgc agggtttacg
     2941 ctaatgcgca ccggcgaatt agctaaagag ttcgccgaaa gacgcctcga tgatctgagc
     3001 tcgcccgaag atccggatat tcatccgctg cgcgagatcg ttgaaggggg tctgaccaaa
     3061 atacgcgcgg aagtcgcacc ctccggaatc gatatcttga atatggaaat tggcattgtc
     3121 gagccgccgg cggatatcaa gggtcatgtc accgaacagc gtattagcaa ttggatgatc
     3181 gaatggaagc gccgtgcgac aatcctgaaa tccgaaggcg acgctattgc gatgcaggcg
     3241 caggaagaag cgcatgccga agcgcaggcg aatatgattc aagcgttgac cgaagggttc
     3301 cgcatcgcga ctgccggcaa tgccagtgtc tccagtgaag tcatcgccat gcgcttcatc
     3361 gatgccctgg aagcgttgat gcattctcag ccggaaaaac aaacggcgaa cgatcgggaa
     3421 gaagaacagc ccgatcaagc ggcacagacg atgttgatcg tccggcagaa ccagaaagag
     3481 taatcggaca tgcctgaaca tttatcgatc gccaacttat taagcttgct ggaaatcgat
     3541 cggcaggatg acgctctgca gctgtgcgct gaagccgaca gcctgtgccg ccgccgccgt
     3601 tacgacgcgg cgatcgaagt ggccgaacgc gccgcgcatc tggcgcgcaa tcattacgaa
     3661 cgcttgggcg tcgcgctgtt atacctcagc agcattcgcg ccagcgcgca tcaagccggc
     3721 caggcagaac aagcgcagcg tgattgcgag cgcgccgttc gagccttgtc gacacagccg
     3781 cacaattacg tgatcgccca aataatccgt ggccagatcg aattggagat cgacgaaagc
     3841 gccaatcggg aagccgcgct ggatgaattc ggcatggccc atttctctct gcaaaaatta
     3901 attctcgatt cgcgagagca caatcgagcg cagcacaccg ccctgtatcg cagcctggaa
     3961 aaatcgctga ctgcgcggat cgaacattta tcgacagctc tggccgatgt cgagccgatc
     4021 gaggttgcgc cgaaacaacc ctcagccgat caatcgacgc ccgttccggc atttgccgat
     4081 caagccacca ttcccacgcg gcccggtcaa gcgacaccgc cgccgattaa gctggccgtc
     4141 ccgacaaaac tcatctggcc gtcgaccgag ttgcccagca tcgaagtcgt tgcggcgatc
     4201 ggcggcgcga catttgattt tatcgaagtc aatcagttat caatcgaagg acgcgattac
     4261 aaagtccagc ccatcgtgcc ggtttcatcg ggcagcgcct cgctgcgttt acagcacggc
     4321 caacagtata tgacgctcca gatcgatggg gccaacgatc aacgcgtgtt gatccgccgc
     4381 caggatcgac ccgatcaacg gcggcagttc atcgtcgtag cggattccag ccaacatcaa
     4441 gcctggatcg acgatgccga atcgaccgtg ccgtttaagg tagttcacat catcggcgca
     4501 gagcgtgaat ggaatattcg aaatggctca gatacttcac ccatcaatga gaacgaacta
     4561 cgcatcatcg gcatcgtcga agcgatcttg acgccggctg aaagtaccga gccgctgatc
     4621 gatctggaac aagtggcggc ctaaacgcct cgccgatact gggtgctggc tgttggcgcg
     4681 gcaattgaac tgccgccttg tcccgcactc caactgctgg acaaacactg agaccattca
     4741 agctcatggc tgaagaccct aactccaaac tccctccccc tcaacccatt cgcttagcca
     4801 acggcaatat taacttcgtc tatcgcgatc ttaacggaca gttcatttcc gaagcgcgct
     4861 atcacgaaat gaaaatgctg ttgaccaacg gccatggcga tctttcaacg ccgcacaagc
     4921 tgcgcgcccg gctgcatcgc cagctcgacg aatcgactgc gctgcaagat ttgaaacgcg
     4981 atctcgcggc gcatcgcaca gctcacaagc gcctgctgtt gaatgacgcc gatctcagcg
     5041 gtctgcccct cacgcatatg gatttgcatg acgcggattt gatcaacgcc aaattgatgg
     5101 aaaccaattt caccgatgcg aatctgcgca actcaaattt acgcggcgcc gatctcaccc
     5161 aagcaatcct gcggcgcacc gatcttgctc aatccgatct cagcagcgcc gaattgcgct
     5221 tcgccgatct gcgcgagacg aaactgaaag atgcgcaatt gatcggcacc caggccgatc
     5281 gggccaactt cagccgatcg gatttacgat cagccgtcat gtccgataag acttcactcc
     5341 aaaacgtcaa tctgactgaa gcactgctca atcatgcgca gctagccgga gtgaacttca
     5401 gcggtgcaca gttgaaaggt gccgatctgc gttggtcgaa attggcgaaa gcaaatctct
     5461 ccaaagcgca attgatcaac gcccgactgg aaggcgtcga tctcagcggt gctgatctac
     5521 gcggtgcaaa cttgaaaggc gcgcagctgc gcgaaaccaa tttgcatggc agcctgatcg
     5581 ataacgacac gctgctcgat cctgaagcgc ggcgcaggtg gaacgcggcc aatttgcaaa
     5641 accccaaagc atcgaccggg cggggatcat tcaccggcgc gatcttgcac ggcgcacaat
     5701 tcgccgggtt cgatctgcgc ggatcggatt tcacgcgcgc cgatctggcc caggctaatc
     5761 tgcatggcgc gcaactgcaa ggtgtatcgc tcatcgaagc caatctcgaa tcagcggatt
     5821 tgcacaacgc gcaattagag cgggccaatc tcaacagcgc gtacctcggc gatgcgaact
     5881 tgaagaatgc gaatctgcgt gacgccgatc tcacgaatgc cgatctgcgt aacgctaaac
     5941 ttcaa
//
LOCUS       DASRTJ010000631         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_133798,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000631 DASRTJ010000000
VERSION     DASRTJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133798"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFF70_12875"
     CDS             complement(<1..405)
                     /locus_tag="VFF70_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZY45631.1"
                     /translation="MNFSDFNLDARLLHNVHKMGYETPTPIQDAALPAGLAGKDILGS
                     AETGTGKTAAYLLPILQRFLNSTDRPRNARALILVPTRELALQVVDHAEQLSKGSPVR
                     VVAIYGGVSLINQEQQLRRGVDIIIATPGRLLD"
     gene            complement(825..2102)
                     /locus_tag="VFF70_12880"
     CDS             complement(825..2102)
                     /locus_tag="VFF70_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45632.1"
                     /translation="MNTRKTTAPVKRNAYSVYLWMEGITSLASWLAFTVSIIYQVTTV
                     GLNPLQLVLVGTTIEVTAFIFEVPTGVVADTISRKLSIVVGLILVGCGFVLEGSIPRF
                     ETILVAQVVAGIGYTFTSGATEAWIADEVGETKATRAFLRGSQVSYIGALIGTAGAVA
                     LGSVAINLPIIIGGVIMILVGVVLIVIMPETGFKPTPREDRTTFQSMLHTFKSGVKLV
                     RGKPVLISILVLGLVYGMYSEGFDRLWGAHFLQDIALPAIGSLQPVVWFGIIRVVSLL
                     LSIGLIEFASRKVNTDSHGLVARAVLALNVSMVIGLLTFAVTGNFVIALLAYWLTSAA
                     RRTLNPVYTAWVNQHLESSVRATVISMTAQIDALGQMIGGPIVGVIGTAFGVPSALFA
                     SGMILSIALPLLIRTIRRDQLSLVNAIDPIAIE"
     gene            complement(2099..>3091)
                     /gene="rsgA"
                     /locus_tag="VFF70_12885"
     CDS             complement(2099..>3091)
                     /gene="rsgA"
                     /locus_tag="VFF70_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZY45633.1"
                     /translation="DAPQRGSVVKKTFGQYFVRADQRIVPCSITSKLRKNLLYPTADS
                     SSITRHVVAVKDIESVDPIAVGDNVEFTMIDEASGMIVNVLPRRNKLSRPAIDGGRRM
                     KSGGQVLEQVIAANIDQVISVMPIANPAPSWDLLDRYLLTAEAANILPLICFTKIDLM
                     LDAAIPNEIKVYESLGYPVLLTSSNTGQGIGAMKAALKDKVSVLWGKSGVGKTSLLNA
                     IQPELGLRVIEVNRHNSEGRHTTSHMEMFDLEFGGSVIDTPGVRTFKLWDVVDTDLAH
                     FMPDLRSYLGQCKFGANCSHQHEPGCAIKAALEAGRIDRRRYESYLAMREYFSE"
BASE COUNT          720 a          869 c          827 g          675 t
ORIGIN      
        1 atcgagcaag cggcccggtg tggcgatgat aatgtccacg ccgcgccgca gttgttgttc
       61 ttgattgatc agggatacgc cgccataaat cgcgacgacg cgcacgggcg aacctttgct
      121 cagttgctcg gcatgatcga ccacttgcaa agcaagttcg cgcgtcggca ctaagatcag
      181 cgcgcgggca ttgcgcggtc gatcggtgct gttcaagaat cgctgcaaaa tcggcagcag
      241 ataagccgcc gttttgccag tgccggtctc ggccgatcct aaaatatctt tgccggctaa
      301 accggcgggc agggccgcgt cttgaatcgg cgtcggcgtc tcgtagccca tcttgtgcac
      361 attatgcagc aggcgtgcat cgagattaaa atctgagaaa ttcattgaaa ctcctagggg
      421 cggcaaaagt ttttgccgat gtgaacagcc gcggggacac gtcgggtcag gggagtaaaa
      481 acaaacgccg cggggttcaa gaccgggcgg cgcgaacgaa ccggatttac ttgctctcta
      541 acgtcgcaaa ctataacatg gattcgatga tccgtcaata cgcattgatc gattgggttg
      601 gggcggcagt ttaactgctg ccccaacttg cgttgtcccg ttttctcgat tgttgttccc
      661 gcacccggcg gctaatcgat ctaaaaacta atcagagccg ctaacctcaa tggccggtgt
      721 gatcaagttt cttcatttgc tctccgactg cgtcctcgcg gtcggcaact acggtgaaac
      781 gcccgcgcag acgtgggctt cgagccatga gccacatgaa tgaatcattc gatggcaatt
      841 ggatcgattg cgtttacaag tgataattga tcgcgtcgaa tggttctgat caacagcggc
      901 agcgcgatcg acaaaatcat tccgctggcg aaaagcgctg atgggacgcc gaacgcggtt
      961 ccgatcacgc ccacgattgg cccgccgatc atctgtccca acgcatcgat ctgcgcggtc
     1021 attgagatca ctgtcgcgcg cacgctcgat tcgagatgct gattcaccca tgcggtatac
     1081 accggattca acgttcgacg agctgcagac gtaagccagt aagccaacag cgcgatcaca
     1141 aagttgcccg tgaccgcaaa agtgagcaag ccgatcacca tcgagacatt caacgccaac
     1201 acggctcgcg cgaccaggcc gtgactatcg gtgttgactt tgcgtgaggc gaattctatt
     1261 aacccgatcg ataacaacag actgactacc cggataatgc cgaaccatac cacgggctgc
     1321 aaactgccga tcgcgggcag ggcaatgtct tgcaagaagt gcgcccccca caagcgatcg
     1381 aagccttcac tgtacatacc gtagaccaag cccaatacca agatcgaaat gagcaccggc
     1441 ttgccgcgca ccaacttcac gccgctcttg aacgtatgca gcatcgattg aaacgtcgtt
     1501 cgatcttctc tcggcgtggg cttgaagcca gtttccggca tgatcacgat cagcacgacg
     1561 ccgaccagga tcatgatcac accaccgata atgatcggta aattgatcgc cacactgcca
     1621 agcgcgactg cgcccgcagt gccgatcagt gcgccaatat agctgacttg tgaaccgcgc
     1681 aggaaagcgc gggttgcctt ggtttcaccg acttcatccg cgatccaggc ttcggtagcg
     1741 ccgctggtga aggtgtaacc gattccggcc acaacttgcg cgactaggat cgtttcgaag
     1801 cgcgggatcg atccttcgag gacaaagccg caaccgacca ggatcaagcc aacgacgatc
     1861 gatagcttgc ggctgatcgt gtcagcgacg acgccagtcg gaacttcgaa gataaaagca
     1921 gtcacttcga tagtcgtgcc gactaagacg agttgtagtg gattcaggcc aaccgttgtt
     1981 acttggtaga ttatactaac ggtgaacgct aaccatgagg cgagtgatgt aatgccttcc
     2041 atccagagat aaacagagta agcattgcgc ttgacgggcg cagtcgtttt tcgagtattc
     2101 attctgagaa atactcacgc atcgccaggt aactttcata acgccgccga tcgatccggc
     2161 ctgcctcaag ggccgctttg atcgcgcagc cgggttcgtg ttgatgcgaa cagttcgcgc
     2221 caaacttgca ctgccccaga tacgatcgca gatcaggcat gaaatgcgcc agatcggtat
     2281 ccacgacgtc ccagagtttg aaagtgcgga cgcccggtgt atcgatcacg ctgccgccga
     2341 attccagatc gaacatctcc atgtgcgaag tggtgtgccg gccctcgctg ttatgtcgat
     2401 tgacttcgat cacccgtagg ccaagttccg gttgaatggc attcagcaaa ctggttttcc
     2461 cgacgcctga tttgccccac aacacactca ccttatcttt caacgctgct ttcatcgcgc
     2521 cgataccctg accggtattt gagctagtca acagcactgg ataaccgagg ctctcgtata
     2581 ctttgatctc attgggaatc gcagcgtcta gcatgagatc gatcttggtg aagcagatca
     2641 atggcagaat gttagcggct tcggcggtga gcaaataccg atcgagcaaa tcccagctcg
     2701 gtgcaggatt ggcgatcggc atgacgctga tcacttgatc gatgttggcc gcgatgactt
     2761 gctccaaaac ttgtccgccg cttttcattc gccgcccgcc gtcgatcgcc ggtcgcgaga
     2821 gtttattgcg acgcggcaag acattgacga tcatgccgct agcctcatcg atcatcgtga
     2881 actcgacatt atcgccaacg gcgatcggat ctaccgattc gatgtccttc actgcgacga
     2941 catgccgcgt gatcgaggat gaatcagcgg tcgggtagag caaattcttt cgcagcttgc
     3001 tcgtgatcga acacgggacg atgcgctgat cggctcgcac gaaatattgt ccaaacgttt
     3061 ttttgacgac gctgccacgt tgaggcgcgt c
//
LOCUS       DASRTJ010000632         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_361855,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000632 DASRTJ010000000
VERSION     DASRTJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361855"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFF70_12890"
     CDS             <1..766
                     /locus_tag="VFF70_12890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZY45634.1"
                     /translation="VCYDDHARPPLPPISGGAADGKDIVLSAQDGNQFSAYIAYSAQP
                     TGPQVVIFPDIRGLHQFYKELALRFAEIGVRALAIDYFGRTAGLTARDESFEWAPHVE
                     HMTIPTFLRDVTAAIDYLHSLSKADRPTYVIGFCRGGTLALHTGAEKFDLAGIISFYA
                     GLSRPIPGAKGSTFEQAAKNRYPLLGLFGGADAGIPASDIQQLDQHLDQAGVKHELIT
                     YPGATHSFFDRRAVEFADASADAWTRMLKFMNVSTG"
     gene            complement(857..1183)
                     /gene="trxA"
                     /locus_tag="VFF70_12895"
     CDS             complement(857..1183)
                     /gene="trxA"
                     /locus_tag="VFF70_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZY45635.1"
                     /translation="MATPTVVTDETFQQRVLNSDKPTLVDFWAIWCGPCRMVAPVVDE
                     IARENEGKLNVVKLDVDDNQNTAMAYGVMSIPTLILFKNGQVAERIVGYKPKADLMKK
                     LAQHLG"
     gene            complement(1262..2050)
                     /locus_tag="VFF70_12900"
     CDS             complement(1262..2050)
                     /locus_tag="VFF70_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45636.1"
                     /translation="MSAIIIDNALIHYEALGRGKPLIFLHGWLGSWRYWMPTMEALSD
                     RYRTYALDLWGFGDSDRNTLHYDMESYVTLVEQFMAQLGILRAAFVGHSLGAAIAVLS
                     GIRSVERVDRVMAVSIPVGDTNISKRLTATGGSLLDRVLGRRAPTELNEVELEAAKTD
                     PLAVANSAKSLMLLEMRRAFERLKMPTLLVNGEKDPFINSFQLDDLHTADDNLRQISL
                     TESGHFPMLDEANKFQRLLRDFLETKPMDLGALALKDEWRRRMH"
     gene            complement(2068..>2318)
                     /locus_tag="VFF70_12905"
     CDS             complement(2068..>2318)
                     /locus_tag="VFF70_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45637.1"
                     /translation="LILMDVRMPKMTGYEACKALKAMPAMRHIPVVFLSAKGQESEIQ
                     QGVEAGAEEYILKPFAPDELTKQIKSVLERVAQRQAKA"
BASE COUNT          520 a          652 c          587 g          559 t
ORIGIN      
        1 cgtgtgttat gacgatcatg cacgaccacc ccttccgccg atttccggcg gcgcagcgga
       61 tggcaaagat attgtgttat cggcgcagga cggcaatcaa ttttccgcgt atattgctta
      121 ctcggctcag ccgacgggac cgcaagtggt gatcttcccc gacattcgcg gcctgcatca
      181 attctacaaa gagctcgcgc tgcgctttgc cgaaatcggc gtgcgcgcac tggcgatcga
      241 ttacttcgga cgcacggcgg ggctcactgc gcgcgatgag tctttcgagt gggcgccgca
      301 tgtggagcat atgacgatcc cgactttcct gcgtgatgta acggccgcga tcgattatct
      361 tcacagcctg agcaaagccg atcggcccac atacgttatc ggtttttgtc ggggcgggac
      421 attggctttg cataccggcg cagagaaatt cgatctcgca ggcatcattt cgttttatgc
      481 cggtttgagc cgtccgattc ccggcgcaaa gggcagcacg tttgaacaag cggccaagaa
      541 tcgatatcct ctgcttggct tgttcggcgg cgcggacgcc gggattccgg ccagcgacat
      601 ccaacaactc gatcagcatt tggatcaagc cggcgtcaaa cacgaactga taacttatcc
      661 gggtgcaacg cacagcttct tcgatcgacg cgctgtggag tttgcggatg cgtcggccga
      721 tgcgtggacg cgcatgctta agttcatgaa tgtatccacg ggatgaagac gatactgatt
      781 atcgagtctt gtaaataaaa gtagggcggc cattcaatgg ctgccctaca ctgttttttt
      841 tacatgcccc gattagctaa cccaagtgct gagcgagttt cttcatcaaa tcggctttgg
      901 gcttataacc gacgatccgc tcggccactt ggccgttctt gaacagaatc agcgtcggaa
      961 tactcatgac cccataggcc atcgccgtat tctgattgtc gtctacatcc agcttcacga
     1021 catttaactt gccttcgttc tcgcgtgcga tctcatcgac gaccggcgcc accatgcgac
     1081 atggcccgca ccatatagcc caaaaatcga ccagcgtcgg tttatcggaa ttcaatacgc
     1141 gctgctgaaa agtttcgtca gtaactacgg ttggagttgc catgtttcta atcctttagg
     1201 taatggctgg ttgcccagtg tataacgctt attgctgacg gctccgtgtg aatttatttt
     1261 cctaatgcat acggcgtcgc cattcgtcct tgagcgccag cgcgcccaag tccataggct
     1321 tcgtctccag aaaatctcgc agcaaccgtt ggaacttgtt ggcttcatcg agcatcggga
     1381 aatgcccgga ctccgtcaag ctaatctgcc gcaggttatc atcggcagta tgcaggtcgt
     1441 ccaactgaaa tgaattgatg aaaggatctt tttcgccatt gaccagcagc gtcggcattt
     1501 tcaagcgttc gaaggcgcgg cgcatctcta acaacatcaa tgatttggcg ctgtttgcaa
     1561 cggctaatgg atctgtttta gcagcttcaa gttcaacttc attcagttcg gtgggtgcgc
     1621 gtctgcccag tacacgatcg agcagtgagc cgccggtagc ggtcaaccgt ttggaaatgt
     1681 tcgtgtcgcc caccggaatg ctgaccgcca tcacccgatc gacacgctca accgatcgga
     1741 tgccggataa taccgcaatc gccgcaccta atgaatggcc gacaaacgcg gcacgcagaa
     1801 tgccgagctg cgccatgaac tgttcgacca gcgtcacata ggattccata tcgtagtgta
     1861 gcgtattccg atcagaatcc ccgaagcccc acaaatcaag cgcataggtg cgatatcgat
     1921 ccgacaaagc ctccatcgtc ggcatccaat agcgccagga ccccagccag ccatgcagaa
     1981 agatcaacgg tttgccgcgg cccagagctt cgtaatgaat tagagcgttg tcaatgataa
     2041 tcgcgctcaa gaacagttca actcctgcta agctttcgct tggcgctgtg ccacgcgctc
     2101 caacaccgac ttgatctgtt tggttaattc atctggcgca aacggtttga gaatgtattc
     2161 ttctgcgccg gcttcaacac cttgctgaat ttccgactcc tggcccttgg cagataaaaa
     2221 cactaccgga atgtgccgca tggctggcat ggctttcaac gccttgcaag cttcataacc
     2281 cgtcatcttg ggcatccgca catccatcag aatgagat
//
LOCUS       DASRTJ010000633         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_171364,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000633 DASRTJ010000000
VERSION     DASRTJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171364"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VFF70_12910"
     CDS             complement(<1..1479)
                     /locus_tag="VFF70_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HZY45638.1"
                     /translation="MSLTKIVEDKTHWLGRRVFSIEQLNIEKLLYILLIGAAILTRFA
                     NLGDRVMSHDESLHVYFSWNLYKGLGFSHTPLMHGPFKFELTALIYSLFGASDFTARI
                     SVAVFGVALVALPYFLRRWLGRTGAISTAFMLLISPSIWYHARYIRDEAYMLVWGMLL
                     VWAMFSYLRTRSNKWLYFLAIIVALMYTTMEAAFIFSAIFGIFLIGVLILDLSRRADF
                     WSKTVVKTIVALVVAVIVAGAVVILHALILGAIGLGPGDPSPFPVPPVPIQPGLPIDL
                     SSQLQFILQTIGGLLKVLLSLLIPLGLFGFAAYKWFKYMLPDPLRESSAFDLALIILT
                     LSLFMLSAGALTVLNSIWMNILRTPFVDVNFFADGNFPTTDVGLVLRLAVVFGAFAAV
                     SIALGLWWDRRRWLISTGIFIGIGVTLFTTVFTNGVGLGTGFVGSLGYWLAQQGVARG
                     SQPIYYYFIVTPIYEYLPILISIAATIFYLVRWLRRRGSAADA"
     gene            complement(1568..2575)
                     /locus_tag="VFF70_12915"
     CDS             complement(1568..2575)
                     /locus_tag="VFF70_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZY45639.1"
                     /translation="MNFAMWGKALQVIPRISKDEWNKLDVISKWLISTRAAVLIMTFI
                     SATIAGLLALRDGKFMLVNYLLLSLGLILAHATNNLLNDYTDHKRGVDKDNYFRSQYG
                     PQPLEHGLMTEKQLLTYAAVTGLLALAAGAYLVVSHGTLALILLGLGAFFVLFYTWPL
                     KYIGLGEITVLLVWGPLMVGGGYFVIAGDWNWNVVIASLPYALGVTTVIFGKHIDKFT
                     EDKAKHIHTLPVILGEKFSRYAVIGMMLLSYVSVIYLVAIGFYSPIMLIVLFALIVFK
                     DTWKVYRQPRPTEKPDNYPADAWPIWFVGYSFVHNRRFGLLYLAGLIGEVLLRVVFRV
                     I"
     gene            complement(2613..4400)
                     /locus_tag="VFF70_12920"
     CDS             complement(2613..4400)
                     /locus_tag="VFF70_12920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45640.1"
                     /translation="MTSEFVVIDQAAESRSMPRTNAGVSVETALYSVVFLMGTLLRWL
                     SLGLVPLSLREAEQALAALNNTSLPVGGSPLLFSFNQILFGLFGNSLGDAGPRLGAAV
                     IGTALILLPWLYRDRIGRVGALAASTMLAISPTFVFASRLIDGTIVVVACASAALGFG
                     LRFIRSHRSIDLVGLAISIGVGLTSGPSIVTLLIVIGLGSSVVLRWIASKEADEVGSI
                     RQSIQRVQIRRAAWWGGGAFILVATVALTNPSAIRFEPENFSAWLQAFRATPTISAAL
                     FLESAIIYEPLIICVGLLGLFAVLVRVDGTSVLLALWAVGSTMIVLLQPGHQAVDILL
                     ALTPLTLLAGMLIQSLTDSMRRHAAFLREGLVWLIGLPLAGYLVLLAASTAISHQGGS
                     SVFLGVTINPLLSFIVTAALLLLLISAVFVIVIGAGAMLRAASSVIMVVLALISIGSA
                     WSVNQLHVADPRELLWGPIATTSDVGDLRSALHAASDRFTGDPNQISISVVAVQPNPI
                     LEWYLRDFKRVKTDVSPDVTAQAVIVPQAISSTVSLGNYYGASFAIRSIWSLDSLQPN
                     GILRWWLYRDADRPAAFETVTVWIKANQP"
     gene            complement(4397..>5244)
                     /locus_tag="VFF70_12925"
     CDS             complement(4397..>5244)
                     /locus_tag="VFF70_12925"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZY45641.1"
                     /translation="LTLGLVAIILFWRARQPQRSSESDGALDQSSSSGIDFILLLFAV
                     GLLLTFVVEFLFVIDGFGSRMNTVFKLYYQAWAMWGVASAFGLYYLMHESKALNSIIR
                     ALMGAICGIFIVAGLVYPLLAIPAKMEASTPTLDAVQSTAASLPDESAAIEWLNRFAP
                     DTAIILEAPGDEYNAGTSRVSTWTGLSAVVGWSGHESQWRGTCDLQCPRADDVKIIYS
                     TTNDETTLSLLNKYNVSYVYIGPNERRLYPPAALSKFDRLLPAVFRQGLVVIYQVPAQ
                     VTSDK"
BASE COUNT         1465 a         1500 c         1311 g          968 t
ORIGIN      
        1 ggcgtctgca gctgatccgc gccgccgcag ccatcgaacg agatagaaga tcgtcgcggc
       61 aatcgaaatc aatatcggca ggtattcata gattggggtg acgatgaagt agtagtagat
      121 tggctgtgag ccgcgcgcga caccttgctg cgccagccag taacccagcg atccaacaaa
      181 gccggtgccc aggccgacgc cgttcgtaaa aacggtggtg aacagtgtga cgccaatgcc
      241 gataaaaatt ccggtactga tcaaccagcg gcgtcgatcc caccacaggc cgagtgcgat
      301 cgagacagcc gcaaatgctc cgaagaccac cgccaggcgc aagaccaagc ccacatcggt
      361 cgtcgggaaa ttgccgtcgg caaagaaatt gacgtcgaca aatggcgtgc gcaaaatatt
      421 catccagatg gaattcaata cggtcagtgc cccggccgac agcatgaaca gactcagcgt
      481 caaaataatc agcgccaagt cgaaagcgct cgattcgcgc aatggatcgg gcagcatgta
      541 tttgaaccat ttgtaggcgg caaaaccaaa gagtcccagc ggaatgagta agctcagcag
      601 tactttgagc agaccgccga ttgtttgcag aatgaattgg agttgcgagc tgagatcgat
      661 cggcagaccc ggttgaatgg gcacaggcgg cactggaaac ggcgagggat cacccggacc
      721 taagccgatc gcgcccagaa ttaaagcgtg caggatcacc acagcacccg ccacgattac
      781 cgccacaacc agagcgacga tagttttcac gacggtcttt gaccaaaaat ccgcgcgccg
      841 ggacaaatcc agaatcagca cgccgatcag aaatatgccg aagatcgcgg aaaaaatgaa
      901 ggccgcttcc atcgtcgtat acatcaacgc cacaatgatc gcgagaaagt acagccattt
      961 attcgaacgc gtacgcagat agctgaacat agcccacacc agcagcatgc cccagactaa
     1021 catatacgct tcatcacgaa tgtagcgggc gtgataccag atcgatggcg agatgagaag
     1081 catgaacgcc gtgctgatcg cgcccgtgcg tcctagccag cggcgtagga agtagggcag
     1141 cgccaccagc gcgacgccaa aaacggccac cgagatgcgc gccgtaaaat cactcgcgcc
     1201 gaacaagcta tagattagcg ccgtcagttc gaacttgaac gggccgtgca tcaacggtgt
     1261 gtgcgagaag cccagccctt tgtaaagatt ccacgaaaag tagacatgca aactctcgtc
     1321 atgactcatg acgcgatcac ccaagttagc gaaccgcgtc aagatcgccg cgccgatcag
     1381 caggatgtac agcagcttct caatatttag ttgctcgatc gagaatacgc gccgaccgag
     1441 ccagtgggtc ttgtcttcga caattttggt taaactcata ggaatctctg atctcggtca
     1501 cagacggcag accgccgacc gcagttgaat gttgattcac ttacctgctg acgctagact
     1561 gcattaatca aatcacacga aacacgacgc gcagcaacac ttcgccaatc agacccgcca
     1621 gatacagcaa gccaaagcgt cgattgtgaa cgaacgagta gccaacaaac cagatcggcc
     1681 aagcgtccgc tggataatta tccggctttt cagttggtcg aggttggcga taaaccttcc
     1741 aggtatcttt aaacacgatt agcgcgaaca acacgatcaa catgatcggc gaataaaaac
     1801 caatcgccac caaataaatg acggacacat acgacagcaa catcatgcca atcaccgcat
     1861 agcgagaaaa tttctcgccc aagataacgg gcagcgtatg aatgtgcttg gctttatcct
     1921 cagtaaattt atcgatgtgc ttgccgaaga tcacggtggt cacaccgagc gcgtaaggca
     1981 aactggcgat tacgacgttc cagttccaat cgcccgcgat gacaaaatat ccaccgccaa
     2041 ccatcaacgg cccccagacc agcagcacag tgatttcacc gaggccaata tatttcagcg
     2101 gccaggtata gaacagtaca aagaatgcgc cgagcccaag cagaattaag gctaacgtgc
     2161 catgcgagac aactagatat gcccccgcag ctaaggcgag taaaccggtc accgctgcat
     2221 acgtgagcag ctgcttctcg gtcatcaagc catgctccaa cggctgcggg ccgtactgcg
     2281 atcgaaagta attgtcttta tcgacgccgc gcttgtgatc ggtgtaatcg ttcagcaagt
     2341 tattcgtggc gtgcgccaag atcaagccta gcgacaacaa aagataattg accaacataa
     2401 attttccgtc gcggagcgcc agcagtcccg cgatcgtagc cgagataaag gtcatgatca
     2461 gcacggcggc gcgggttgag atcaaccatt tggaaatcac atccagttta ttccactcgt
     2521 ctttgctgat gcgcggaata acttgcagtg ccttgcccca cattgcgaaa ttcatttgaa
     2581 gcctccgcta tactatccaa acttaaaaat tattacggct gattggcctt gatccacacc
     2641 gtaaccgttt caaacgcggc gggccgatcg gcgtcacgat acaaccacca gcgcagaatg
     2701 ccatttggct gcaaactatc taaactccag atcgatcgaa tggcaaacga tgcgccgtaa
     2761 taatttccca gtgacactgt cgatgaaatg gcttgcggca caataaccgc ttgagcagtg
     2821 acatcgggtg acacatcagt cttcacccgt ttgaaatcgc gcaaatacca ctcaaggatc
     2881 ggattgggct ggaccgctac aacgctaatc gagatttgat ttggatcacc ggtaaaccga
     2941 tcggaggcag cgtgcagcgc tgaacgcaga tcgcccacat cgctcgttgt ggcgatcggt
     3001 ccccacagca actcgcgcgg atcggccaca tgcagctgat tcactgacca cgcgctgccg
     3061 atcgaaatta aagccagcac aaccatgatc acgcttgacg ccgcgcgcag catcgctccg
     3121 gcgccaatga cgataacgaa cactgcgctg atcaacagca gcagcagcgc ggcagtcacg
     3181 ataaacgata acagcggatt gatcgtgacc cccaagaata cgctgctccc cccttggtgg
     3241 ctgatcgcgg tgctagccgc caacagtacc agataaccag ccagcggcaa accgatcaac
     3301 cacactaacc cttctcgtaa aaatgctgcg tgccgccgca tagaatccgt caacgattga
     3361 atcaacatac cagccaacaa cgtcaagggc gttaaagcta acagtatatc cactgcttga
     3421 tggccgggct gcaagagcac aatcatcgtc gagccgaccg cccataaggc caacagcact
     3481 gatgtaccat caacgcggac gagcacggcg aataagccga gcaggcctac gcagatgatc
     3541 aacggctcat aaataatcgc gctctcaaga aagagcgccg cgctgatcgt cggcgtggcg
     3601 cgaaaggctt gcagccacgc tgaaaaattt tccggttcga atcgaatcgc ggaagggttg
     3661 gtcagcgcca ctgtcgcgac caaaataaat gcccccccac cccaccacgc tgcgcgccgg
     3721 atttgaactc gctgaatcga ttgccggatc gatccgactt cgtcggcttc cttcgatgca
     3781 atccaacgca ggacaacgct ggatccaagg cctatgacga tcagcagcgt tacgatcgat
     3841 ggcccactcg ttaaccctac cccgattgag atcgccagcc caacgagatc gatcgagcgg
     3901 tgtgatcgaa tgaagcgcag gccaaagcca agcgccgctg acgcgcacgc gactacaacg
     3961 attgtgccat cgatcaagcg cgaggcaaac acaaacgtcg gtgaaatggc cagcatcgtt
     4021 gaagcagcta acgcgccgac gcggccgatt cgatcgcgat acaaccacgg cagcaggatc
     4081 agcgccgtgc cgatcaccgc tgcgccaaga cgcggcccag catcaccgag actgttgcca
     4141 aagagtccaa acaggatctg attgaagcta aacaacaacg gactgccgcc caccggcaaa
     4201 gatgtattgt tcagggcagc taaagcttgt tcggcttcgc gcagggagag aggtacaaga
     4261 cccagactta accagcgtaa tagcgtgccc atcaagaaga cgaccgaata caacgccgtc
     4321 tcaaccgaga cgccagcatt cgtgcgcggc attgatcttg attcagccgc ttgatcaatg
     4381 acaacaaatt cactcgtcac ttatcactcg tcacttgtgc tggcacttga tagataacaa
     4441 ccaagccttg ccgaaagaca gcaggcaaca gccgatcgaa tttgctcaag gccgcaggcg
     4501 gatagagccg tcgctcgttc ggaccaatat atacatagct tacgttatat ttattgagca
     4561 atgataacgt cgtttcatca ttagttgtcg agtaaatgat tttcacatca tcggcgcgcg
     4621 ggcactgaag gtcgcacgtg ccgcgccatt gactttcatg acctgaccaa ccgacgacgg
     4681 ctgataatcc cgtccaggtg gaaacgcgcg atgtgccagc attgtattca tcgcccggcg
     4741 cttccaagat aatcgccgta tcgggcgcga aacgatttag ccactcgatc gcggcggatt
     4801 cgtctggaag cgatgccgct gttgattgta ccgcatccag tgtcggagtg gacgcctcca
     4861 ttttggctgg gatagccaac agcggataaa ccaatccagc gacgatgaag atgccacata
     4921 tcgcgcccat cagagcccgt ataatagagt tcaatgcttt cgattcgtgc atcaggtaat
     4981 acaaaccaaa cgccgaagcc acgccccaca tcgcccatgc ttgatagtag agcttgaaga
     5041 ccgtgttcat ccgcgagcca aaaccgtcga ttacgaacag aaactcaacc acaaacgtca
     5101 gcagcaaacc gacggcaaaa agcaacaaaa tgaaatcaat accgctagat gaagactgat
     5161 cgagcgcgcc gtcggattca gaggatcgtt gaggctgccg cgcgcgccag aataaaataa
     5221 ttgcgactaa gcccagggta agac
//
LOCUS       DASRTJ010000634         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_288050,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000634 DASRTJ010000000
VERSION     DASRTJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288050"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFF70_12930"
     CDS             complement(<1..500)
                     /locus_tag="VFF70_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZY45642.1"
                     /translation="MTHHLTLRIYRYKPGSDPHYDNFSIEVSDEANVLDAIERAWDDQ
                     DRSLMFRHACHHASCGSCGLRVNEVEQLPCVTPVTDYPDGATLKIDPMRNFPLVTDLA
                     VDAAPLYERTREVGTPIMRESEPLRENGLRVLPEGIADFNRFENCIECGLCLSACPSM
                     AADPYY"
     gene            complement(686..1861)
                     /locus_tag="VFF70_12935"
     CDS             complement(686..1861)
                     /locus_tag="VFF70_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY45643.1"
                     /translation="MSDSNRNNRFHVIVIGAGSTGSATAHDLALRGFKVTVIDRGEIA
                     SGTTGRNHCLLHSGGRYAVKDPEGAIECIEENMIIRKIMPDALELNDGLFVATDESDL
                     AFKAQFLEGCAACKIPAREIPIERALQIEPYLNPKILAAIQVPDGVFEPYRFCLAFLA
                     TAKKNGATVLPYHLVTGLRMSGIAVTGVKVRDNRTMKDYEIGADLVVNASGPWADKIA
                     IMANVDVPVKPTAGVMVTIGQRLNQMVVNRLNKPSDGDIVVPQRQTSIIGTTSWPVDD
                     MDAIEIPREHVEKMIAMGERLIPMVRRLPIRGVMAVARPLISKPGVDEREVSRTFECF
                     DHKHDGVENFVTISGGKTTTARAMAEKVSNIVCEKLGVQAECHTRETRLESFRLYYN"
     gene            complement(1999..>2325)
                     /locus_tag="VFF70_12940"
     CDS             complement(1999..>2325)
                     /locus_tag="VFF70_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZY45644.1"
                     /translation="VLDAMNKDSGVNLTALKVDGGMVFNNTLMQFQADILGVPVIRPT
                     VAETTALGATYAAGLAVGFWNAVEDLRANWGKDHEWKPMMDPKKRDSLYKGWLKAVTR
                     TFDWVE"
BASE COUNT          523 a          659 c          621 g          522 t
ORIGIN      
        1 aaataatatg gatcagccgc cattgatgga caagccgata ggcataagcc acactcgatg
       61 cagttttcaa aacgattaaa gtcggcaatc ccttcaggca gaacacgcag gccgttttca
      121 cgcagcggtt cagactcgcg catgatcggc gtgccgactt cgcgcgtgcg ctcatataat
      181 ggcgcagcat cgacagccag atcggtgacg agcggaaagt tgcgcatggg atcaatcttc
      241 aacgtcgcgc cgtcgggata atcggtgacg ggtgtgacgc acggcagttg ctcgacttcg
      301 ttgacgcgca ggccgcacga tccacacgag gcatgatggc aagcgtgccg gaacattaac
      361 gatcgatctt gatcgtccca cgcgcgttcg atcgcgtcga gcacgttagc ttcgtccgac
      421 acttcgatcg aaaaattatc gtagtgcgga tcgctgccag gtttatagcg atagatgcgg
      481 agagtcaggt gatgagtcat aaacgattga tccgtgacga gtgacgagtg aaaagtgacg
      541 cgtgtagttt actcatcact gatattcttt agtcaaatag gctctcgttt caagggcaac
      601 ttaagtctgt caccctgagc gaagcgcagg gtctcgcgct cttaaccgag atgcttcgct
      661 tcgctcagcc tgacacgcga aagtcctagt tgtaatacag acggaacgat tcaagccgcg
      721 tctcgcgtgt atgacattcc gcctgcacac ctaatttttc gcagacaatg ttcgaaactt
      781 tttcagccat cgcgcgcgcg gtggtggttt tgccgccgga gatcgtcaca aaattttcga
      841 cgccatcatg tttgtgatcg aagcactcga acgtgcggct gacttcgcgc tcgtcgacgc
      901 cgggcttgct aatcaacggt cgagcgacgg ccatcacgcc acgaatgggc agccgccgca
      961 ccatcgggat taagcgttca cccatcgcga tcattttctc gacatgctcg cgcggaattt
     1021 cgatcgcgtc catatcgtcg accggccacg atgtggtacc gatgatcgat gtctggcgct
     1081 gcggcacgac gatgtcgcca tcggacggtt tgttgagtcg attcacaacc atttggttca
     1141 gccgctgccc gatcgtgacc atcacgcccg ccgtgggttt gacgggtaca tcgacattcg
     1201 ccatgatcgc aatcttgtcg gcccacggtc cagaggcgtt caccactaag tctgcaccga
     1261 tttcataatc tttcatcgtg cgattgtcgc ggactttcac acccgtcacg gctatgcctg
     1321 acatcctcag gccagttact aaatgataag gtaaaactgt ggcgccgttc ttcttcgccg
     1381 ttgctaaaaa ggctaagcag aatcgataag gttcgaaaac accatcaggc acttgaatgg
     1441 ccgcgagaat tttcggattg agatacggtt cgatctggag cgcgcgctcg atcggaattt
     1501 cgcgcgcggg aatcttgcac gcggcgcagc cctccaggaa ctgggctttg aaagcgagat
     1561 cagactcatc ggtggccaca aataaaccgt cgttgagttc gagcgcatcc ggcatgatct
     1621 ttcgtatgat catgttctct tcaatacact cgatcgcgcc ctctggatct ttcacggcat
     1681 agcggccgcc gctgtgcagc aagcaatgat tgcgacccgt ggtgccggac gcgatctcgc
     1741 cgcgatcaat cacggtgacc ttgaagccgc gcagtgccag atcgtgcgcc gtcgccgagc
     1801 cggtggatcc cgcaccaatg acaatcacat gaaagcgatt attccgattc gaatcggaca
     1861 ttgagtccgt gctccttcaa tgcaaggcat gcggatgccg agcgacaact tcaaaagata
     1921 cgccggcttc agccaaccct aaggcccccg ctgaagccgg cgttgttcaa cagtgtgata
     1981 ctcgatcagc tacaaaattt actcaaccca atcgaatgtg cgggtcacgg ctttgagcca
     2041 gcccttgtac aatgaatcac gtttcttggg atccatcatc ggcttccact cgtgatcctt
     2101 gccccagttg gcacgcagat cttcgacggc gttccagaag ccaactgcca gaccggctgc
     2161 ataggtagca ccgagggcag tggtctcggc cacggtcgga cgaatgaccg gcacgcccag
     2221 gatgtcggcc tggaattgca tcaaggtatt gttgaacacc atgccaccgt cgaccttgag
     2281 cgcggtcagg ttaacgcccg agtctttgtt catggcatcc agcac
//
LOCUS       DASRTJ010000635         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_362506,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000635 DASRTJ010000000
VERSION     DASRTJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362506"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..911
                     /locus_tag="VFF70_12945"
     CDS             177..911
                     /locus_tag="VFF70_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45645.1"
                     /translation="MWNGKKVSVVFPVYNEEAGLGQAIEDFFRYGWVDEIVVVDNNSK
                     DRSAEIAKATPARLVTETQQGYGFALRRGLKEASGDYIILAEPDGTFMGKDVLRLLAY
                     ADDFDMVCGTRTTREMIWAEANMGIFLRVGNWIVAKLLEFLFNGPSFSDCGCTLRLIS
                     ADAARRLNDHFTVGKSHFLPEMVILGLIAHLRIIEVPVNYRGRLGQSKITGSFKTAWR
                     VGWNMIRLIVSYRIRAWFGRAPKITA"
     gene            920..2617
                     /locus_tag="VFF70_12950"
     CDS             920..2617
                     /locus_tag="VFF70_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45646.1"
                     /translation="MPKSNRLDLNRGMWIALIASTIGLTLACLRMWWQPILADEWDFY
                     RAMIDWLNNRALIPHPQAYIHFAQLNLSIFGTSIGSARLAGVFSAIASVWLIPVLVHY
                     AWPDHPQRDRFTVIAIILTAINPLTLQNAMILDIDNTLLIPATMILIIAWAATLHSSR
                     RTRIIVVSLTLAFNLWVKLPTPPLIMASLGLYHLARREWKRAGEIILTSLLGLAIFSL
                     TFMIYSALSGYQLSYFGMTFGRAGSFFNLRDLITRFPQGLGVFVMWLSLPLTILLLIA
                     VIESARRLWHKRTTPADAIILGVIIVGLFYPLVYVPAWGYPRYQAPIVPLISTLVAAL
                     IVPYTLSLPKSVVRGLLILGAILLALNLWLLPDPLYPIYQVTFEGDLYDITHRLLGGL
                     KVVAEIGVPIILLLIAGTIYARRQKLDGGAARRRILSLLTVVTLAALSFTQVNADYST
                     RYRYTYNYADYWWSVREAQSLGLNANIMAIKDALWESGLPGEEIYPYLCADCSPSLVD
                     TIHARRIDALIWTTKEASRSGGLTDDPKFNAALDACYSRSTRGVFIVYLLKNATPCK"
     gene            2705..>3160
                     /locus_tag="VFF70_12955"
     CDS             2705..>3160
                     /locus_tag="VFF70_12955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45647.1"
                     /translation="MRILHIYKDYSPVLGGIENHVRVLAEAGVQRGHEVTVLAASLDR
                     SLHVETLNGVKLIKTPRWINISSAPISPSMFRWARKIGRDADIVHLHFPYPLGEMAHL
                     FSGSRAKTVITYHSDIVRQKFLRMIYRPFLWRILRKADRIIATSDRYRDT"
BASE COUNT          677 a          909 c          821 g          753 t
ORIGIN      
        1 aagtccaacg ggtcttcggg ctgcgtaaac cgatcgagtt ggcagagggc atcgcgcaca
       61 tggctgactg gaccaaagcc gccggcccgc gccagagcaa atatttcggc gagatcgaaa
      121 ttccacgcaa cctcccgccg agctggttac aacgttaacg gagaggcttc tcacccatgt
      181 ggaacggcaa aaaagtcagc gttgttttcc ctgtttataa tgaagaagca ggcctcggtc
      241 aagcgatcga ggattttttc cgctacggct gggtcgatga gatcgtcgtc gtcgacaaca
      301 actccaaaga tcgcagcgcg gaaatcgcca aagcgactcc cgcccggcta gtcacggaga
      361 cacaacaggg ctacggtttc gcgttgcggc gcggcttgaa agaagcgagc ggtgattata
      421 ttatcctcgc cgagcccgat ggcaccttca tggggaaaga tgtgctgcgc ttgctggcgt
      481 atgccgacga cttcgatatg gtgtgcggca cccgcacgac ccgtgaaatg atttgggccg
      541 aagcaaacat ggggatcttt ttgcgcgtcg gcaattggat cgtcgccaag ttgctggaat
      601 ttctattcaa tggcccgtcg ttctcggatt gcggctgcac gctgcggctc atcagcgctg
      661 atgcggcacg gcgcttgaat gatcacttca cggtaggtaa atctcatttt ctgccagaga
      721 tggtcattct aggactgatc gcgcacctgc gcattattga agtgccggtg aactatcgtg
      781 gtcgcttggg acaatccaag atcaccggtt ccttcaaaac cgcctggcgc gtcggctgga
      841 atatgatccg cttgatcgtg agttaccgaa ttcgagcgtg gttcgggcga gcgccgaaaa
      901 tcaccgccta aggatcgctg tgccaaaatc gaatcggctt gatctcaatc gcggcatgtg
      961 gatcgcactc atcgcctcga cgatcggact gacgctggcc tgtctgcgta tgtggtggca
     1021 gcccatcctc gccgacgaat gggacttcta tcgcgcgatg atcgattggc tcaataaccg
     1081 cgcgttgatc ccacatccac aagcatacat tcacttcgcg cagctcaatc tttcgatctt
     1141 cggcacctcg atcggttcgg cgcgcttagc gggcgtcttc agtgcaatcg cgtcggtttg
     1201 gctcattccg gtcttggttc attacgcctg gcctgatcat ccgcagcgcg atcgcttcac
     1261 agtgatcgcg atcattctca cggcaatcaa tcccctaacg ctgcaaaatg cgatgatcct
     1321 cgacatcgac aatacgctgc ttattccggc gacgatgatc ctgatcatcg cctgggcagc
     1381 caccttacac tcatcgaggc gcacccgcat catcgtcgtc agtctgacgc tggccttcaa
     1441 cttatgggtc aaactgccga cgccgccact aatcatggca agcctcggtc tgtatcacct
     1501 cgctcgccgc gaatggaaac gcgcgggtga gatcattctc acttcgctat tgggcttagc
     1561 gatcttcagc ctgacattca tgatctattc cgcattgagc ggttatcaac tcagttactt
     1621 cggcatgaca ttcggtcgag cgggcagctt cttcaatctg cgcgatctga tcacacgctt
     1681 tccgcaaggt ctgggcgtct tcgtgatgtg gctctcgctg cccttgacga ttctactgtt
     1741 gatcgctgtg atcgaaagtg cgcgccggct gtggcacaaa cggacaacgc ccgcagacgc
     1801 gatcatattg ggcgtgatca tcgtcgggct cttctatccg ttagtgtatg ttccagcgtg
     1861 gggctatccg cgctatcaag caccgatcgt accgctgatc tcgacgctcg tcgccgcgtt
     1921 gatcgtgcct tacacattaa gcctcccaaa atcggtcgtg cgcggattgt taattctcgg
     1981 tgcaatttta ctcgccctga atctgtggct gctacctgat ccactctatc caatctatca
     2041 agtgaccttt gaaggcgatc tctacgatat tactcatcga ttgttgggcg gcttgaaggt
     2101 cgtcgccgag atcggcgtgc ccatcatact gttgttgatc gccggaacga tctatgcacg
     2161 gcgccaaaaa ctggatggcg gcgcagctcg gcggcgaatt ctcagcttat taacggtggt
     2221 cacacttgcc gcgctgtcct tcacgcaagt caacgccgat tacagcacac gctatcgcta
     2281 cacctacaac tacgccgatt attggtggag cgtccgcgaa gctcaatcgc taggattgaa
     2341 tgcgaacatc atggcaatca aagacgctct gtgggaatcc ggcttgcctg gcgaggaaat
     2401 ttatccgtac ttgtgcgcgg attgttcgcc atcattagtg gatacgatcc atgcacggcg
     2461 tatcgatgcg ctcatatgga ccaccaaaga agcttcgcgc tccggcggcc tgacagacga
     2521 tcccaaattc aacgcggcgc tcgatgcgtg ttactctcga tcgactcgcg gcgtgtttat
     2581 tgtgtatttg ttaaagaatg ctacaccttg caaatagaag ggcgaggtaa ccacatccga
     2641 ctaatacttc tttaccacca acggagtatt tacatcggcg acccgctcat ctactctaat
     2701 tattatgcgc atccttcata tctataaaga ttactccccg gttctcggcg gtattgaaaa
     2761 tcacgtccgc gtgctggccg aagcaggcgt gcaacgcggc catgaagtca cggtattagc
     2821 cgccagcctc gatcgatcgc tgcacgttga aacgctcaac ggcgtgaagt tgatcaagac
     2881 cccgcgctgg atcaacatct cctccgcgcc aatcagtcca tcgatgttcc gatgggcacg
     2941 gaagatcgga cgggatgcgg acattgtgca tttacacttt ccctatccgc tcggcgagat
     3001 ggcacatctc ttcagcggca gccgcgccaa aacagtcatt acttatcaca gcgacatcgt
     3061 ccggcaaaaa tttctgcgga tgatctatcg gccgtttctc tggcgcattc tgcgtaaagc
     3121 cgatcggatc atcgccacca gcgatcgcta ccgcgacact
//
LOCUS       DASRTJ010000636         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_288231,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000636 DASRTJ010000000
VERSION     DASRTJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288231"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFF70_12960"
     CDS             complement(<1..546)
                     /locus_tag="VFF70_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45648.1"
                     /translation="MQTRWTIMWGAVLVLIGALLLGQQFGLFGPMQLSFAVFLFGIPA
                     LMFLLTYIFDRRQWWALIPGCILLGLAAVVFNEANHFVSSLVSGAIFLFSIALPFWLI
                     YMSKRWWWAIIPAGVMTVLAIMPIISNDMLQGQFVGGTFFLGLAAVFALVRLATRSDP
                     NMGWAWWPAAILGIFGALTILL"
     gene            631..>1552
                     /locus_tag="VFF70_12965"
     CDS             631..>1552
                     /locus_tag="VFF70_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HZY45649.1"
                     /translation="MITPPSIDQWDAFVNARGHILQSSAWAELKSHFGWSADRICVMR
                     DDEIVAGASILFRSLPLHLGTLAYIPKGPIVDLDDHELTHELLGGFDRLMKQHRSILL
                     KIEPDRSADESLRTRLADLSFQVSPQTIQPPRTITIDISRSEEEILAAMHQKTRYNIR
                     LSAKKDVSVREAQESDLPAFNALMQATGNRDKFDVHSAEYYSAAYGLFVPDDRARLFV
                     ATYQEKIIAGIFVFALRDRAWYFYGASGDVERERMPNHALQWAAIRWAKSRGCTEYDL
                     WGVPDENEATLEVQHLDRSDGLWGVYRFKRG"
BASE COUNT          393 a          447 c          407 g          305 t
ORIGIN      
        1 caataagatc gtcaatgcgc cgaagatgcc caggatcgcc gcaggccacc acgcccaacc
       61 catgttcgga tcagaccggg tcgccaatcg gactaacgca aaaactgccg cgaggccgag
      121 gaagaaagtc ccgccgacga attggccttg caacatatcg ttggaaatga tcggcatgat
      181 cgccagcacc gtcatcacac ccgctggaat gatcgcccac caccagcgct tcgacatata
      241 gatcaaccag aacggcagcg cgatgctgaa gagaaagatc gcaccggaga ccaatgagga
      301 cacaaagtga ttggcttcat tgaagacgac cgccgcgagt cctaacaaaa tacagcccgg
      361 aatcaacgcc caccattgcc gccgatcgaa aatgtacgtt aacaagaaca tcaaggccgg
      421 aatgccgaac aagaagaccg caaacgataa ctgcatcggc ccgaacaggc cgaactgctg
      481 tcctagcagc aatgcgccga tcaacaccag caccgcgccc cacataatgg tccaacgtgt
      541 ttgcattttg cacctccaat cgatgatgcc gatcatttta gcatttcgat tcgcgcaggt
      601 caatgatgaa tcgctggtat aatcgcgccg atgatcacac cgccttcgat cgatcagtgg
      661 gacgccttcg tcaacgcgcg cggccacatc ttgcaatcct ctgcctgggc tgagctcaaa
      721 tcgcacttcg gctggagcgc cgatcgaatt tgcgtgatgc gcgacgacga aattgtcgcg
      781 ggcgcatcga tcttgttccg gtcgctgccc ctgcacctgg gcactttagc gtacatcccc
      841 aaaggcccga tcgtcgatct cgacgatcac gaactcaccc acgaattgct cggcggcttc
      901 gatcgattga tgaagcagca tcgatcgatc ttattgaaga tcgaacccga tcgatcagct
      961 gatgaatctc tcagaacgcg attggctgat ctcagctttc aggttagccc gcagaccatc
     1021 caaccgccgc gtaccatcac gatcgatatt tcccgcagcg aagaagaaat tctggcggcg
     1081 atgcaccaga aaacacgcta caacattcga ttgtcggcga agaaagatgt cagcgtgcgt
     1141 gaagcgcaag aaagcgatct gccagcattt aatgcgctca tgcaagccac cggcaatcgc
     1201 gataagttcg atgtgcattc agccgaatat tactcagcgg cttatgggtt gttcgtgccc
     1261 gacgatcgcg ccagactttt cgtcgcaacg tatcaggaaa aaatcatcgc gggaattttt
     1321 gtgtttgcct tacgagatcg agcgtggtat ttctacggcg cgtcgggcga tgtggaacgc
     1381 gagcgcatgc caaatcacgc cttgcagtgg gccgcgattc gctgggccaa atcgcgcggt
     1441 tgcacggagt acgatctgtg gggtgtgcca gatgaaaacg aggccacgct tgaagtgcag
     1501 catctcgatc gcagcgacgg gttgtggggc gtttatcgat tcaagcgcgg ct
//
LOCUS       DASRTJ010000637         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_288460,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000637 DASRTJ010000000
VERSION     DASRTJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288460"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VFF70_12970"
     CDS             complement(<1..1418)
                     /locus_tag="VFF70_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45650.1"
                     /translation="MAGNRRLFQNAQRRGNKFYDEMAWDKALAEFQAAAEEFPDDLGV
                     LDKLADLNERLGLLDKAAEVYITIAGLKSREGLTNEAIDYWQRAARLDNNNLDTHKHL
                     AYAFITQNKPKQAVREQLAMARIYQMQHATGDALLSVQAAYKLDPTNVDVLKALELLK
                     ATTTQQTEKAAQADRAADDTIDSSEQHGSPVDITREKAMSDLAESIFEETPSAARVTP
                     LMSKDEIDSLISQALDQQTTNDIEAAIGTYNRILSAGVQMPAVNFNLGLLYQENVRLN
                     EAIEQFNQSVNHGEYRLGSLFALGELARAKGRVNEALNYFLEALKIIDLQTVRLDQAD
                     DLIQVYDSLAETYAAKVEPQQAASFVSSLVEFLSNKGWEDKVVQARRRLDSLTEDGTP
                     AMSLAEILTVRDGDQLLQSMSLMHELARRGKYYSALEEAYIAIQHAPDYLPLHLRLAE
                     VIWKSGQQPQAISKFLTVANTCL"
     gene            complement(1499..2512)
                     /gene="argF"
                     /locus_tag="VFF70_12975"
     CDS             complement(1499..2512)
                     /gene="argF"
                     /locus_tag="VFF70_12975"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZY45651.1"
                     /translation="MNHFLSLTDLTSDQLASLINLAVKLKQEWRTGGNAPLLKGKTLG
                     MVFTKPSLRTRVSFDMAMLHLGGQALYISPNEISLGQRESAADVARVLSRYVDVIMAR
                     VFSHRDVVELAQHSRVPVINGLSDYSHPCQGLADLLTIYEKLGTLQGIKIAYVGDGNN
                     VANSLIFGAALSGSNFSIASPAGYEITGDVVARAKTMANKTGSKLYFSHDPAEAVREA
                     DVIYTDVWTSMGQETESQKRLEVFAPKYQINAALVGKAKRNAIVMHCLPAHRGQEITD
                     DVVDGSHSVVFDQAENRMHAQKAILVQLLAPQRRRSSRVLKTVKAKAKKATRKKIGVK
                     KKR"
     gene            2665..3465
                     /locus_tag="VFF70_12980"
     CDS             2665..3465
                     /locus_tag="VFF70_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45652.1"
                     /translation="MNLRFILRGSISVLALTLIVMGIGLRVTDLAFRPLDPTPPPPTI
                     LAPRGELPIDPGGLIEFARYGDSDFGVGCGFLLQLPSGSLIGVTTAHSLLFNNPASPL
                     QRIAFGVANRSGYVAIFDTFWGMPGVARSGEDMTVDYVLLKPIDATSVDRSLVLQPDP
                     RGAPQPGERVMLLSGLADGQGGRIIFEGTVQSVDATAVSVLMDSSFDPSGLSGSPFVS
                     EFTGRVVGMTIATSYRANRVLLSLHPIGSIVRLAQSAIEFPKIGEYRR"
     gene            complement(3482..>3670)
                     /locus_tag="VFF70_12985"
     CDS             complement(3482..>3670)
                     /locus_tag="VFF70_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZY45653.1"
                     /translation="VILYSIATPFLPGSMLSVIPGILSAILFGVRTHLVDEMLIAELS
                     GYPEFTQRTSFRLLPGAW"
BASE COUNT          761 a         1039 c          973 g          897 t
ORIGIN      
        1 agacaggtat tcgccaccgt caaaaacttc gagatggctt gcggctgctg gccgcttttc
       61 cagatcactt ccgccaagcg caaatgcagc ggcaaataat ccggtgcatg ctgaatcgcg
      121 atataggctt cttctagtgc gctgtagtat ttgccgcgtc gcgccaattc gtgcatcaat
      181 gacatcgatt gcaacagctg atcgccgtcc cgcacagtca agatttccgc caggctcatg
      241 gcgggtgtcc cgtcttcggt caggctgtcc aagcggcggc gggcttgcac gactttatct
      301 tcccagcctt tgttgctcag aaattcaacc aagctgctga cgaagctggc ggcttgctgc
      361 ggttcgactt tggcggcgta agtctcggct aaactgtcat agacttggat cagatcatcg
      421 gcttgatcga gccgcacggt ttgcagatcg ataatcttca gcgcttccag aaaataattc
      481 agcgcttcgt tcacgcgacc tttggcgcgc gcgagttcac ccagcgcgaa cagactgccc
      541 aaacgatact cgccgtgatt gaccgattga ttgaattgtt caatcgcttc atttagccgg
      601 acgttctctt gatacaataa gcccagattg aaattcacgg cgggcatctg cacgccggct
      661 gagagaatac gattgtacgt gccaatcgct gcttcaatat cattggttgt ttgttgatcg
      721 agcgcctggc tgatgagtga atcgatctcg tccttgctca tcagcggtgt gacgcgggca
      781 gcactcggcg tctcttcaaa gatcgattcg gcgagatcac tcattgcttt ctcgcgcgta
      841 atatcgacgg ggctgccgtg ctgctcgctg gaatcgatcg tgtcatccgc cgcccgatcg
      901 gcttgcgcgg ctttctcggt ttgctgcgtc gtcgtcgcct tcaggagttc gagcgccttc
      961 agcacatcga cattcgtcgg atcgagcttg taagcagcct gcactgatag aagtgcatca
     1021 ccggttgcat gctgcatttg ataaatgcgc gccatggcca gctgctcgcg caccgcttgt
     1081 ttgggcttat tctgcgtgat gaaagcataa gccagatgct tatgtgtatc aagattatta
     1141 ttatccaacc gcgcggctcg ctgccaataa tcaatcgctt cgtttgtcag accctcgcgc
     1201 gatttgaggc ccgcgatagt gatgtaaacc tcggcggctt tatctaacaa gcccagccgc
     1261 tcattcagat cggcgagctt atcaaggaca ccaagatcgt ccgggaactc ttcggcggcg
     1321 gcctgaaact ccgctaaagc tttgtcccaa gccatttcgt catagaactt attgccgcgc
     1381 cgctgcgcgt tttgaaatag acgtcgattg ccggccatag ttgattatcg tatatcgtgg
     1441 tgaggggtgc atcggataca aataggacac gcacccctca ttactcgtca tctctgactt
     1501 atctcttttt cttgacacct atttttttgc gagttgcctt ctttgctttc gccttcaccg
     1561 ttttcaacac acggctcgat cggcgacgct gcggcgcaag cagctgcacc aagatcgctt
     1621 tctgtgcatg catccggttc tccgcctgat cgaatacgac cgagtgcgac ccatcaacca
     1681 catcgtccgt gatctcttgg ccgcgatgcg ccggcaggca gtgcatcact atcgcgttgc
     1741 gtttcgcttt gccgaccagc gcggcattga tttgatactt cggcgcaaaa acttccagcc
     1801 gtttctgcga ttcggtttcc tgccccatcg aggtccagac atccgtgtat atcacatcgg
     1861 cttcgcggac ggcctcagcc ggatcatgcg agaaatataa cttgctgccg gtcttatttg
     1921 ccatcgtttt cgctctggct accacatcgc ccgtgatttc gtagccggcc ggcgacgcga
     1981 tcgagaagtt cgagccgctt aacgccgcgc cgaaaatcaa cgaattcgcc acgttattgc
     2041 cgtcgcccac atacgcgatc ttgatgcctt gcaacgtgcc cagcttttca tagatggtca
     2101 gcaggtcagc caacccttgg catggatgtg aataatcact gagaccgtta atgaccggga
     2161 cacgtgaatg ctgagctagt tcgacgacat cgcggtgact gaacacgcgc gccataatca
     2221 catcgacata gcggctgagc acgcgtgcca catccgccgc actttcacgc tggcccaacg
     2281 aaatttcatt cggcgaaata tacaacgcct gcccgcccaa atgcagcatc gccatgtcga
     2341 acgacacgcg cgtgcgcaga ctgggcttgg tgaagaccat gcccaacgtc ttgcctttca
     2401 gcagcggcgc attgccgccg gtgcgccatt cttgcttgag ttttaccgct aaattgatga
     2461 ggctcgccaa ctgatcggag gtcagatcgg tcaacgacaa gaaatgattc acagcgaatc
     2521 ctccggaaat ttaagatcga tcgcaagtat agcacaagac ggcgtgcgct ggtagggtca
     2581 tggctgtttt tgatcgataa atgacgctgg tggtgcagct gaaacccgat tggctgatcg
     2641 ggctgatcgg acgataatta ccgcatgaac ctgcgattta ttttgcgcgg atcgatcagt
     2701 gtgttggcgc tgacgctcat cgtgatgggc atagggctgc gcgtaaccga tctcgctttc
     2761 cgcccgcttg atcccacacc gccgcccccg acgattcttg cgccgcgcgg tgaactgccg
     2821 atcgaccctg gcggtctgat cgaatttgcc cgctacggtg attcggactt tggcgtcggt
     2881 tgtgggtttt tgttgcaact ccctagcggc agcttgatcg gcgtgacgac cgcgcacagt
     2941 cttttgttca acaatcctgc ctcgccattg cagcggatcg cgttcggcgt cgccaaccga
     3001 tcgggttatg tggcaatctt cgatacattc tggggcatgc ccggcgtcgc gcgatcaggc
     3061 gaagacatga ccgtcgatta cgtgctgctc aagcccatcg acgcgacatc agtcgatcga
     3121 tcacttgtct tgcaacccga tccgcgaggc gcgccacaac ccggcgagcg agtgatgttg
     3181 ctcagtggct tggccgacgg gcagggcgga cgaataattt tcgaaggcac ggttcaatca
     3241 gtcgacgcca ccgctgtgtc ggtgttgatg gatagctcat ttgatccgag cggcctgagt
     3301 ggctcaccgt tcgtcagtga attcacaggc cgtgtggtcg gtatgacgat tgccacgtct
     3361 tatcgcgcca atcgcgtatt gttgagcctg catcccatcg gctcgatcgt gcggctggct
     3421 caatcagcga tagagtttcc gaaaattggg gaatatcgaa ggtaagacat ctggcgaggt
     3481 tttaccacgc gcccggcagt aatcgaaatg atgtgcgctg tgtgaactca gggtagcccg
     3541 ataattctgc tatcagcatt tcatctacca aatgagttcg taccccgaac aagatcgcag
     3601 acaagattcc ggggatcact gacaacatcg atccgggcaa gaacggagtc gcgatcgaat
     3661 acaggatgac
//
LOCUS       DASRTJ010000638         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_209248,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000638 DASRTJ010000000
VERSION     DASRTJ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209248"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1873)
                     /locus_tag="VFF70_12990"
     CDS             complement(<1..>1873)
                     /locus_tag="VFF70_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45654.1"
                     /translation="VQASIGFDYSIATAPGSNTPFAMGLVQALVTLLGLIALVVTWRR
                     DQNKSLRVFALIGLGLSTLMITPLSRMVWDHLPLLSFAQFPWRFLSIQALFTSLIIGY
                     VPSIFQARRLSFVICHLLIGLLLIASILLPLHPDYLPIRADEITPDRLQLYEAFTSNI
                     GTTIRAEYLPRTVIPRPYTGPALIDPSLPPQAIVSSGAATSTRLSRGAIDQVWQVTVI
                     SNAATLDFPLLYWPGWIASIDGQSVEVKAAPDLGYVQIDVPPGEHRIEFSLGRTPIRV
                     IGELVSIVALIVLAILLWSHIRAYPHGLRLFTLRLIYGLVVTAIIIVLGSLAQAGNVV
                     LANENDLTMDFDSKPWLHHNPGGVDFGGAAKLIGYEIGEISDQSVNLSLNWDVATSQA
                     VSVTIALATPSTHLFNDPSPIDQESVMIRSGTSTVHLTSPYRLATGMYYMRVQVGSID
                     QYLKPIWITSDQTLTGAHLFGKLTPQIGLAAVQVQHLDPDRLEVLLSWSISGSIAANY
                     GISLRLHASTGEPSATLDTQPGYGFQPTSAWQTGALNDAYTLSLPNELPRVGVYSLDA
                     ILYRVASKEEVGRTTLDGLRLDSTYAWTAIEPPLRDLVERPVPIRVDAIFGDQIELLG
                     "
BASE COUNT          488 a          512 c          492 g          381 t
ORIGIN      
        1 accccagcaa ttcgatctga tcgccgaaga ttgcatcgac ccgaatcggc acaggccttt
       61 caaccagatc acgcagcggc ggctcgatcg ctgtccatgc ataagttgaa tcgagacgaa
      121 gtccatcgag cgtcgtgcgg cccacttctt ccttcgacgc cacccgatag agaatcgcat
      181 ccagcgaata cactccgaca cgcggcagtt cgttcggcag tgataacgtg taagcatcat
      241 ttagcgcgcc agtttgccac gcgctggtgg gttgaaagcc gtaacccggt tgcgtatcca
      301 gcgttgcaga aggttcgccc gttgaagcgt gcagccgcaa tgaaattccg taattcgcag
      361 cgatcgatcc gctgatcgac caactcaaca acacctccaa tcgatcggga tcaaggtgtt
      421 gcacctgaac cgccgccaat ccgatctgtg gcgtgagctt cccaaataga tgtgcgccgg
      481 ttaatgtttg atcgctcgta atccagattg gcttgagata ttgatcgatc gatccgactt
      541 gcacacgcat gtaatacatt cccgtcgcta atcgatacgg cgaagtcagg tggacagtgc
      601 ttgtgcccga tcggatcatc acgctttctt gatcgatcgg acttgggtcg ttgaacaagt
      661 gcgtcgaggg cgtggccagg gcgatagtca cgcttacagc ttgtgatgtt gccacatccc
      721 aattcagcga cagattaaca gattgatcgc ttatctcacc aatctcatac ccgatgagct
      781 ttgccgcgcc accgaaatcc acgccgcccg gattgtgatg cagccacggc ttgctgtcaa
      841 aatccatcgt caaatcattt tcgttggcca agactacgtt gcctgcttga gctaagctgc
      901 ctaatacgat aatgatggcc gtaaccacta acccatagat cagacgaaga gtaaacagtc
      961 ttaatccgtg cggatatgcg cggatatgcg accacaacaa gatcgctaaa acaatcaggg
     1021 caacaatcga aaccagctcg ccaattacgc gaatgggcgt gcgacccaaa ctgaactcaa
     1081 tgcgatgttc acccggcggc acatcgatct gaacatagcc caaatcgggc gcggctttca
     1141 cttcaacact ttgtccatca atcgatgcga tccagcccgg ccaatagagc agcggaaagt
     1201 ccagcgtcgc cgcattcgaa atcaccgtga cttgccaaac ttgatcaatc gcaccgcgcg
     1261 agagtcgggt tgatgtagca gctccgctcg acacgatcgc ttgcggaggc aatgacggat
     1321 cgatcaacgc gggtccggta taaggccgcg gaatcacagt gcgcggcaga tactccgcgc
     1381 ggatcgtcgt gccgatgttg gacgtgaacg cttcgtagag ctgcaatcga tccggcgtga
     1441 tctcatcggc gcgaatcggt aggtagtcgg ggtgaagggg caatagtatt gaagcaatga
     1501 gtagcagtcc aatgagcaaa tgacaaatga caaatgacaa acgacgagct tgaaaaattg
     1561 aaggaacgta accaatgatc aacgacgtga acagcgcttg tatcgataag aagcgccacg
     1621 gaaattgcgc gaacgacagc agcggtagat gatcccacac catgcgcgat agcggcgtaa
     1681 tcattaaggt ggataaaccg agaccgatca gcgcgaagac gcgcagtgac ttattttgat
     1741 cgcgccgcca ggtaacgacg agagcgatca agcctagcag cgtgaccaat gcttgaacca
     1801 gccccatggc aaatggcgtg ttcgaaccgg gcgcagtcgc gattgagtaa tcgaaaccga
     1861 tcgaggcttg cac
//
LOCUS       DASRTJ010000639         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_135047,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000639 DASRTJ010000000
VERSION     DASRTJ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135047"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VFF70_12995"
     CDS             complement(<1..1748)
                     /locus_tag="VFF70_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HZY45655.1"
                     /translation="MTEFQSPYLDLLKNRINLTHIPFSERGSRLLIFRSNQHLSVRLA
                     ERWFKVDPQLSGYRQRPPIIDEWTFTDGDGQPLNLEITSQPHRLDLQTRIGAFTLIFQ
                     DAETLLMTLPAAKCGIDFLANVDQARTDRRGGVLGVTGDIRRNIAYTTNTRLVENLIE
                     PIGAEANQVSLRFDSARGGSLLINITPRLGFNRYIPDPEEAINAAAKRWHDWFAAAPA
                     VAEEYRAQYYYAWWIMRVGLISTRFYTTREAMTPSKIYYVGVWQWDAYFHALAYRHVD
                     KKLAHDQLRIVIDHQRADGMIPDAVHDEGTVTRLSHPIEGDVTKPPLLAWAAWKLYEL
                     DHDREFLDEIYEPIVRWNNWWFEQNDTDGDGLCEYHHPYSSGLDDSPLWDDGMPVEAP
                     DLNTYLFLQLEALSKIAQVIDQTDDAERWAAKAEEMTVRLIRDMWDEQGGYFRANRIV
                     NKEHVQVNVRTPFNLFPLITGRLPKSINDRLIAHLTNEHEFWPRYPVPTVALDDPKFD
                     PLTMWRGPTWVNVNYLLIEGLQRNGYIDLARELRRRTLEMIKQHDDIYEYYQSETGDV
                     PPKAASIFGWSSAVFI"
     gene            complement(1741..>2075)
                     /locus_tag="VFF70_13000"
     CDS             complement(1741..>2075)
                     /locus_tag="VFF70_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45656.1"
                     /translation="RDQIERELTDRSSLRYQVFDRYSQLLKARSNSPAFDPHGVQRVI
                     ECGHMIFAVVRNERVLCLYNVSNERQTISIDPNWRDAVSLIDGRSIVSNSTIDLAPYQ
                     ILWLKRND"
BASE COUNT          457 a          557 c          571 g          490 t
ORIGIN      
        1 tcgatgaaca ccgccgacga ccagccgaag atcgaggcgg ccttcggcgg aacatcgccc
       61 gtctcagatt gatagtattc gtagatatcg tcgtgctgtt tgatcatctc caacgtgcgg
      121 cggcgcagtt cgcgcgccag atcgatgtag ccgttacgct gcaagccttc gatcaacaga
      181 tagttgacgt tgacccaggt cgggccgcgc cacatggtta acggatcgaa cttgggatcg
      241 tcgagcgcca cagtgggcac cggatagcgc ggccaaaatt catgttcgtt ggtgagatgc
      301 gcgatcaatc gatcgttgat ggacttgggc aagcggcctg tgatcagtgg aaacaaattg
      361 aacggcgtgc gcacgttgac ctgcacgtgc tctttgttga cgatccggtt tgcccggaag
      421 tagccgccct gttcatccca catgtcgcgt atcaagcgta cggtcatctc ttcggctttc
      481 gcagcccagc gttccgcgtc gtctgtctga tcaatgacct gcgcaatctt tgataacgct
      541 tcaagttgta agaacaggta agtattgaga tcgggcgcct cgaccggcat gccgtcatcc
      601 cacaatggac tgtcgtccaa gccggatgaa tatggatgat gatactcgca caatccatcg
      661 ccgtccgtgt cgttctgctc gaaccaccaa ttgttccagc gcacgatcgg ttcgtagatc
      721 tcatccagaa attctcggtc gtgatccaac tcgtaaagtt tccacgccgc ccaggcgagt
      781 aatggcggct tggttacatc gccttcgatc ggatgagaca atcgggtgac agtgccttcg
      841 tcatgaaccg catcggggat catgccatcc gcgcgctgat gatcgatcac gatgcgtagt
      901 tgatcgtgcg ccaatttttt atcgacatgc cggtaagcca gcgcatgaaa gtaggcgtcc
      961 cactgccata cgcccacgta ataaattttg gacggcgtca tcgcttcgcg cgtcgtgtaa
     1021 aagcgcgtgg agatcaaacc gacgcgcatg atccaccacg cgtagtaata ctgcgcgcga
     1081 tattcttccg cgaccgctgg ggcggctgca aaccaatcgt gccagcgttt ggccgcagcg
     1141 ttgatcgcct cttcgggatc gggaatatat cgattgaagc ccaggcgcgg cgtgatgttg
     1201 atcagcagtg aaccgccgcg cgccgaatcg aatctcaagc taacctgatt agcttctgcg
     1261 ccgatcggct cgatcaaatt ttcaacgagg cgcgtgttgg tcgtgtacgc aatgttgcgg
     1321 cgaatgtcgc ccgtcacgcc cagtacgccg ccgcgccgat cggtgcgcgc ttgatcgacg
     1381 ttggcgagaa aatcgatccc gcacttcgct gccggcaatg tcattagtaa tgtttcggca
     1441 tcctgaaaga tcaatgtgaa tgcgccgata cgcgtctgca aatcaagtcg atgcggttgc
     1501 gaagtgattt ccaagtttag cggctgtccg tcgccgtcgg taaatgtcca ctcgtcgatg
     1561 atcggtgggc gctgacgata gccgctgagc tgcggatcga ctttgaacca gcgctcggcc
     1621 aagcgcacgg aaagatgttg attgctgcga aagatcaaca agcgcgagcc acgctcactg
     1681 aatggaatgt gcgtgaggtt gatccgattt ttgagtaaat ccaaataggg cgattgaaat
     1741 tcagtcattg cgtttcaacc acaaaatttg ataaggtgcc agatcgatgg tgctgttgct
     1801 tacgattgat cggccatcga tcaagctaac tgcatcgcgc cagttcggat cgatcgagat
     1861 cgtttgccgc tcattcgaaa cgttatatag acacagtacg cgctcgtttc tcacaactgc
     1921 gaagatcatg tgtccacatt ctatcacgcg ttgcacgcca tgcggatcga atgctgggga
     1981 gttcgatcgc gctttcagta gttgtgaata tcgatcgaag acttgatagc gcagcgatga
     2041 tcgatcggtc aactcgcgct cgatttgatc tcgat
//
LOCUS       DASRTJ010000640         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_209339,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000640 DASRTJ010000000
VERSION     DASRTJ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209339"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1471)
                     /locus_tag="VFF70_13005"
     CDS             complement(86..1471)
                     /locus_tag="VFF70_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZY45657.1"
                     /translation="MSSKTTAILGPTFEEMLHPSKIDPKVRAQAVKALKDDPLNPINL
                     YNITWRGEDDQIKYEVLPKQLTGVDAPIVVLYGRDFPTGAHKVGAAYSVLVEKILFGE
                     VNPAKNTVVWPSTGNYGIGGAWVGGRVGTKSIVVLPQGMSKERFQMIESFGASIIKTV
                     GSESNVKEIYDKTHELALDPNTRILNQFEVMGNYRFHYHVTGNTIVELAEVLHKQGIG
                     DGTIAAYCSAMGSAGTIAAGDRLKQAFPDHKIVGLEPTQCPTLYNNGYGSHDIQGIGD
                     KHVTWIHNVMNMDAIMCLDDIECKKGLQLLTEEPGMLLMKEHGVPEAKVKQLSTIFGI
                     SGVCNVLGAIKTAKFYGFGQNDVIVTICTDAIDRYHSVMSDMTETYGQLDRAEALARL
                     DSIFLGQKTDWIHEGTTDMRQQWHNLKYYTWVEQQGKSVEELNEQMHQEWWIAHQQMV
                     GEIDKRITATR"
     gene            complement(1591..2394)
                     /locus_tag="VFF70_13010"
     CDS             complement(1591..2394)
                     /locus_tag="VFF70_13010"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZY45658.1"
                     /translation="MRVLISVDMEGISGVTHWDHVTPDRSEYARFRSIMTDETNAAIE
                     GAFNAGADRVVVVDGHDAGSNILLEKLDPRAKLITGSPAPLSMVEGVQNSDVAMFIGY
                     HARSGTPNAILDHTWSDRVRFLSINDREIGEIGFNAAVCGSFNVPIVMLSGDRAACDE
                     AIELLGHIETVIVKSAVGRLTAECLSINAAHQMIREAAARAIKQHPALFTFTPPMNVI
                     IEFAHTGHIDRALRIPGTRRVSGTRIEFQSADILAAYNTFRAIVAIAGD"
     gene            complement(2466..>2830)
                     /locus_tag="VFF70_13015"
     CDS             complement(2466..>2830)
                     /locus_tag="VFF70_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45659.1"
                     /translation="PPYMPVMGLRLDFLDRNFSIAYSADTSPSDSVVELASGVDLLIH
                     ESSFSATLEPDQDPTFYFHSDARMAGQIAVRAGAKKLALVHLSRLHGDHRRVLTAEAA
                     ESFHGEIFVPDDGDVLKF"
BASE COUNT          619 a          752 c          738 g          721 t
ORIGIN      
        1 gcggtctcat cgcaacgcat cgatcgatga agaagcataa tcgataaaaa acttccgaag
       61 gccggcgact tcggaagtat ttgtttcagc gggtggcagt gatccgctta tcgatctcgc
      121 cgaccatctg ttgatgggcg atccaccact cttgatgcat ctgctcgttc agttcctcga
      181 ccgacttgcc ctgctgctca acccacgtgt aatacttcaa gttgtgccac tgctggcgca
      241 tgtcggttgt gccttcgtga atccaatcgg ttttctgccc caggaagatg ctgtctaaac
      301 gcgccagcgc ttccgcgcga tccagctggc cgtatgtctc agtcatgtcg ctcatcaccg
      361 aatgatagcg atcgatcgcg tcggtgcaga tggtaacgat cacatcgttc tggccgaagc
      421 cgtaaaactt ggcagtcttg atcgcgccca gcacattgca gaccccgcta atgccgaaga
      481 tcgtcgagag ttgtttgact ttcgcttccg gcacgccatg ctccttcatt aatagcatgc
      541 ccggttcttc ggtcaacagc tgcaagccct ttttgcattc gatgtcgtct aggcacatga
      601 tcgcgtccat gttcatgacg ttgtgaatcc atgttacgtg tttgtcgcca atgccttgaa
      661 tatcatgcga gccgtagccg ttgttataca acgtcgggca ttgagttggt tcgaggccaa
      721 cgatcttgtg atcggggaag gcttgtttta atcgatcgcc cgccgcgatc gttccggcgg
      781 agcccatcgc gctgcaatac gccgcaattg ttccatcgcc gatgccttgt ttatgcagca
      841 cctcggcgag ttcgacgatg gtgtttccgg tgacatgata atggaagcga taattaccca
      901 tcacttcaaa ctgattaagg atgcgcgtat tgggatccag cgccagttca tgcgtcttgt
      961 cgtagatttc tttgacattg ctctccgaac cgacggtctt gataatcgaa gcgccgaatg
     1021 attcgatcat ttgaaagcgt tccttgctca taccctgcgg tagcacgacg atcgatttcg
     1081 tgccgacgcg accgccgacc cacgcgccgc cgatgccgta gttgcccgtg ctcggccaga
     1141 caactgtgtt ttttgccgga ttgacttcgc caaataaaat cttttcgact aaaaccgaat
     1201 atgccgcacc gactttgtgc gcgccggtcg gaaaatctcg gccgtacaac accacgatcg
     1261 gcgcgtcgac gcccgtcagt tgtttgggca gcacttcgta tttgatttga tcatcttcac
     1321 cgcgccaggt gatgttgtat agattgatcg gattcagcgg gtcgtccttc aaggctttca
     1381 ccgcctgggc gcgcactttc ggatcgatct tgctggggtg cagcatttct tcaaaagttg
     1441 gaccgaggat tgcagttgtt ttgctggaca tgattcactc cgattggaat tggatattag
     1501 aagtgggatg cttgaggtta gacagctatt gctggttttg gattcgaatt atccgtcatt
     1561 gaaaatggtt cattcttaat tgcaaactgc ttaatctcct gcgattgcga ctatcgctcg
     1621 aaatgtgttg tatgctgcta aaatatccgc gctttgaaat tcgatccgcg tgccgcttac
     1681 acgccgggtg ccgggaattc gcaacgctcg atcgatgtgg ccggtatgcg cgaattcaat
     1741 gatgacattc atcggcggcg taaacgtaaa tagagcaggg tgttgtttga tcgcacgggc
     1801 ggctgcttcg cggatcattt ggtgtgccgc attgatcgat aaacactccg ccgtcaaacg
     1861 gccgacggcc gatttgacta ttaccgtttc gatatgaccc aatagttcga tcgcttcgtc
     1921 gcaggcggcg cgatcgccgc tcagcatcac gatcggcaca ttgaacgatc cgcacacggc
     1981 ggcattgaat ccgatctcgc cgatctcccg gtcgttgatt gataaaaacc gaactcgatc
     2041 cgaccaggtg tgatcgagaa tggcattcgg tgttcccgat cgggcgtgat agccgatgaa
     2101 cattgcgaca tcggaattct gcacgccttc taccatcgac agaggcgcag gcgaaccagt
     2161 gatcaatttc gcgcgcggat ctagtttctc caacaagatg ttgctgcctg catcatgtcc
     2221 atcgacaacg acaactcgat cagcgccggc gttaaacgca ccttcgatcg cggcattggt
     2281 ttcatccgtc atgatcgatc gaaagcgggc gtactcagat cgatcgggcg tgacgtgatc
     2341 ccaatgagtt actccgctga tgccttccat gtcaaccgaa atcaagacac gcatgaattt
     2401 attcgatcaa gaagaaagtt gatttaaaag ttgtgcgtgt tccgatgctg cttgcatact
     2461 gggtattaga atttaagcac atcaccatcg tctgggacaa atatttcgcc gtgaaaagac
     2521 tcagcggcct cggcagttag cacgcggcgg tgatcgccgt gcaagcggct cagatggacc
     2581 aaggccaatt tctttgcgcc tgcccgcacg gcgatctgtc cggccatccg agcgtctgaa
     2641 tgaaaataga aagttgggtc ctgatcgggt tcgagcgtcg cgctaaacga tgattcgtgg
     2701 atcaacaggt cgacgccact ggctaactcg accacgctgt ccgagggtga cgtatcggct
     2761 gaataagcga tcgagaaatt gcgatcgaga aaatcgagcc gcagtcccat aaccggcata
     2821 tacggcggtc
//
LOCUS       DASRTJ010000641         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_289162,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000641 DASRTJ010000000
VERSION     DASRTJ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289162"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2184)
                     /locus_tag="VFF70_13020"
     CDS             complement(<1..2184)
                     /locus_tag="VFF70_13020"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZY45660.1"
                     /translation="MSKYPTLNLETLLKAYSSDLHSPNGAGNGNSVQASREMITKAFA
                     LAEKAHLGQKRSSGEPYVQHCLAVANILTEFHLDAPTIAAALLHDTLEDTTVTRSELK
                     TKFGEEVASLIEGVTKLGQFDQLDSKGERSYDERELESLRKMFLAMVDDPRVVLIKLA
                     DRLHNMRTLGALTEDRRERIAKETLELFAPLANRLGIWQMKWELEDLGFRYLYPDKYR
                     EIAALIDERLPNREDYIDEIIRKLQDKLKAEGIQSAEISGRPKHIYSIWKKMERKGGF
                     DQVYDVRAVRVIVDTVAQCYAVLGIVHSMWQPIRGEFDDYIATPKDNFYQSLHTAVVA
                     DEGKTLEAQIRTHDMHRHAELGIAAHWRYKEGGKSDLKYEEKIKWFRQLMDFRSDISD
                     AKEFVDSLRNDVFQDRVYVFTPKGKLIDLATASTPIDFAYQIHTDVGDRCRGARVNGS
                     LVPLDYQLQTGDRVEILTAKRGGPSRDWLNPNLGYVKTERARTKIKVWFKKQDREANI
                     AQGREILERELRRLSVEALPHDAVAKLFNLDKVDDFFEKIGSNDISSQAIVSKVIEAD
                     RKANPPEEEPVNFVAPSKLEVSDLTVRGTGGLLTTLATCCNPVPGDAIVGYVTRGRGV
                     TVHKRNCVNIVHSSEAERLIEVSWGSKQRTYPVTIKITAYDRDGLLRDVAAVITGGSV
                     NMSSVNVTTNKNIATFLATLEIAGTSQLSTVMDRIERLPNIIEVRR"
     gene            2342..2896
                     /gene="rsmD"
                     /locus_tag="VFF70_13025"
     CDS             2342..2896
                     /gene="rsmD"
                     /locus_tag="VFF70_13025"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZY45661.1"
                     /translation="MRVISGSAKGRNLKAVPGDTTRPITDRVKGAVFNILSSDVIDAR
                     CLDLFGGTGGVGIEALSRGAAHCAFVEKNRAALDVIRDNLQTTHLLDRAELIRSDAFN
                     YLRGAPQPFHYIYIAPPQYQRMWIEALHLIDVQPLWLAEDGEIIVQIHPKEFEELPLN
                     NFEQVDERKYGSTLLIFYEHKPTT"
     gene            3009..3881
                     /locus_tag="VFF70_13030"
     CDS             3009..3881
                     /locus_tag="VFF70_13030"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZY45662.1"
                     /translation="MAVLLLTACESRSSSPLATPTAPFLPTHIPLPTITSTPSATETP
                     TPEPTVAPLTDGWRETRSGVAIREMYQDEQSQRGKVLVVRIDPNQVDFHVRYQPENPQ
                     HVFDWYNDSRSLIVMNSSFFDRFNNAVGQLTTDSVSGGSIHDRMEGAFYLTAVGAAVW
                     PLRGWQKPAGMQIVESIESFPMYLMPGGLLNPDISDDATAARRTAVAVDRSGNVLFIV
                     TAGSAFTLKGLGIWLANSDLNLDTALNFDGGSSSGVAVWTPIGVWGLDSNNHVPAVIT
                     VDPKVLGLTGGEAP"
     gene            3992..>4772
                     /locus_tag="VFF70_13035"
     CDS             3992..>4772
                     /locus_tag="VFF70_13035"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZY45663.1"
                     /translation="MSRDACASPLGMLGWCLFLLSLIALISCSSITSSAPVSITPSDR
                     IPTQTSIPVLTTTPIPTDTGWLTLPSGLEYRELYVTFQNQSDHLRLARVDPSHFNFRV
                     LYAPNQPRQVSEWLASNEAFLLVVNGGYFDPAYHALGQLISDGISVGRNYQGFGGMFA
                     VNLDGTVQIRWNIQQPYVEGEGLKYALQNFPMMIIPGGGSNDQIDDNGELALRSIVGQ
                     DRSGRIIFIVSPSAAFTLSGMSQWLPISDLDLNVALNLDGGT"
BASE COUNT         1012 a         1287 c         1310 g         1163 t
ORIGIN      
        1 ccgtctcact tcgatgatat tcggcagtcg ctcgatacga tccatcaccg tagacaactg
       61 ggatgtgccg gcgatctcca gcgttgccaa aaacgtggcg atgtttttgt tggtcgtgac
      121 attgacactg ctcatgttga cactgccgcc cgtgatgacg gcggccacat cgcgcagcag
      181 gccgtcgcga tcgtaggccg tgattttgat cgtcactgga tacgtgcgtt gcttgctgcc
      241 ccaggagact tcgatcaagc gttcggcttc cgaactatgc acgatgttaa cgcagttgcg
      301 cttatgaact gtcacacctc gaccgcgcgt cacataaccg acgatcgcat cgccggggac
      361 tggattgcaa caagtcgcta aagttgtcag tagaccgccg gtgccacgca cggtcagatc
      421 ggacacctcc agcttcgacg gtgccacaaa attgactggc tcttcctcgg gcggattcgc
      481 tttgcgatcg gcttcaatca ccttgctgac gatggcttgt gaactgatgt cgttgctgcc
      541 gatcttctcg aagaagtcat cgaccttgtc gaggttaaac agtttggcta ccgcatcgtg
      601 cggtaacgct tccacactca agcggcgcag ctcgcgttcg agtatttcac gcccttgagc
      661 gatgttcgct tcgcgatctt gcttcttgaa ccagactttt atcttggtac gcgcgcgttc
      721 agtcttgacg tagcctaagt tgggattcag ccagtcgcga ctcggcccgc cgcgtttggc
      781 ggtgaggatt tccactcgat cgccggtctg caattgataa tcgagcggca ccaggctgcc
      841 attgacgcgc gcgccccggc agcgatcgcc gacgtcggta tggatttgat aagcaaaatc
      901 gatcggcgtg ctggccgtcg ccagatcgat cagcttgccc ttcggcgtga agacgtacac
      961 gcgatcttga aaaacatcgt tgcgcagcga atcgacaaat tctttcgcgt ccgaaatatc
     1021 agatcggaaa tccatcaact gccggaacca cttgatcttc tcttcgtact tgagatcgct
     1081 tttgccgcct tctttatagc gccagtgcgc agcgataccc aattcggcat ggcgatgcat
     1141 gtcgtgcgtg cggatttgag cttcgagggt tttgccttca tcggcgacaa ctgccgtgtg
     1201 cagactttgg tagaagttgt ccttcggggt cgcgatgtaa tcgtcgaatt caccgcgaat
     1261 cggctgccac atcgaatgaa caatgcccag taccgcataa cactgcgcca cggtatcgac
     1321 aatcacccgc actgcccgga catcatagac ttgatcgaag ccgcccttgc gctccatttt
     1381 cttccagatg ctatagatat gtttgggccg gcctgatatt tcggcgctct gaatcccttc
     1441 ggctttcaat ttatcttgca gcttgcggat gatctcgtcg atgtaatctt cgcgattggg
     1501 tagccgttcg tcgatgagcg cggcgatctc tctatattta tcgggatata gatagcgaaa
     1561 gccgagatct tcaagttccc atttcatctg ccagatgccc aaccgattag cgagtggcgc
     1621 aaagagttcc agcgtctctt tcgcaatgcg ctcgcgtcga tcttccgtca gcgcgccgag
     1681 tgtgcgcatg ttgtgtaatc gatcggccag tttgatgagc acgacacggg gatcgtcgac
     1741 catggccagg aacatcttgc gcagcgattc gagttcgcgc tcgtcgtatg atcgttcacc
     1801 ttttgaatcc aattgatcga attggcctag cttggtaacg ccttcgatga gcgaagcgac
     1861 ttcttcgccg aatttagtct tcaattcgct gcgggtaacc gtggtatctt cgagcgtgtc
     1921 gtggagcaat gcggcggcga tcgtcggcgc atcgagatgg aattcggtga ggatgttggc
     1981 gacggcgagg cagtgctgca catacggctc gcccgatgat cgcttctggc ccagatgagc
     2041 cttctccgct aaagcaaatg ctttagtgat catctcacgg ctggcctgaa cgctgttgcc
     2101 attgcccgcg ccgttaggac tatgcagatc ggatgagtaa gccttaagca atgtttcgag
     2161 gttaagtgtc ggatatttgc tcatcgtgaa aatattgtag cacaaatatc aattcgtaat
     2221 gatcaattca caatgaacaa cgatcaatca gggcgggtag cacctgaagt atggtaggat
     2281 tgaagccgac caaggacgaa tgacgaagtt ggtcattcgt cgttcgtctt aaaaagggat
     2341 gatgagagtt atatccggtt cagcaaaagg ccgtaatctc aaagcggtgc ctggcgatac
     2401 gacgcggccg atcaccgatc gggtcaaagg cgcggtgttc aacatcctga gcagcgatgt
     2461 gatcgatgcg cgctgtctcg atctctttgg cggcacgggc ggggtcggca tcgaagcgtt
     2521 gagccgcggc gcggcgcact gcgcgtttgt cgagaaaaac cgcgcggctt tggatgtgat
     2581 cagagataac ttacagacca cgcacttgct cgatcgggct gagctgatcc gatcggacgc
     2641 tttcaattat ttgcggggtg cgccgcaacc atttcactac atttacatcg cgccgccgca
     2701 gtatcagcgg atgtggatcg aggcgctgca cttgatcgac gtacagccat tgtggttggc
     2761 ggaagatggc gagatcatcg tgcagatcca tccgaaggaa tttgaagaac tgccgctgaa
     2821 taattttgag caagttgatg aacggaaata tggcagcacg ctgttaattt tctatgagca
     2881 taaaccaact acttaactgc ggacgccaga gacctgccgc ttcgaggact tgggcaggcg
     2941 cgatgacgcg aagattaata agtttttctt tgcggctccc gccccatctc attttatggg
     3001 gttgcgtctt ggcggtttta cttctcacag cctgcgaatc acgatcttca tcgcccctcg
     3061 ccacaccgac tgcacccttc ctgcccacgc acattccatt acctaccatt acttcgactc
     3121 cctcagcgac tgagacaccc acaccggaac ccaccgttgc acctttgact gatggctggc
     3181 gcgaaacccg atcaggcgta gcgatccgcg agatgtatca agatgaacag agccaacgcg
     3241 gtaaagtgtt ggtggtgcgc atcgatccca atcaagttga ttttcacgtg cgctatcagc
     3301 cggaaaatcc acaacatgtg tttgactggt ataacgacag ccgatcgttg atcgtgatga
     3361 acagcagttt cttcgatcgc ttcaacaatg ccgtcgggca actcaccacc gacagcgtga
     3421 gcggcggctc gattcacgat cgcatggaag gcgcgtttta tttgacggca gtcggtgcgg
     3481 cagtgtggcc gctgcgcggt tggcaaaaac cggcgggcat gcagattgtt gaatcgatcg
     3541 aatcgtttcc gatgtatctc atgccgggcg gcttgttgaa tcccgacatc tccgacgatg
     3601 caaccgctgc tcgccggacc gccgtcgccg ttgatcgatc gggtaatgta ttgttcatcg
     3661 tgactgcggg cagcgccttc actttgaaag gcctgggcat ttggttggcg aatagcgatc
     3721 tgaatctcga caccgctctt aactttgacg gcggctcgtc atcgggcgtg gccgtgtgga
     3781 cgccgatagg cgtatgggga cttgactcga ataatcatgt gcccgccgtc atcacggtcg
     3841 atccgaaagt attgggcttg accggcggcg aagcgccatg agaaatctgg ttgcaagagt
     3901 tacgatcatt gggagagtgg agcgcgtact tcgactgtgt ggcgtgcgag catccagcgc
     3961 ggatgcttca cgcgcactgc gagtgaagca gatgtcacga gacgcgtgcg cgtctccgct
     4021 cgggatgctt ggctggtgtt tattcttgct gagtttgatt gcattgatca gttgcagctc
     4081 gatcacttca agcgcgccgg tgtcgattac gcccagcgat cgcattccaa ctcaaacgtc
     4141 gattccagtt ttaacgacca cgccaatccc gacggacact ggctggctaa cattacccag
     4201 cggcctcgaa tatcgcgaac tgtacgtgac tttccaaaac caatcggatc atttgcgcct
     4261 agcgcgtgtc gatccgtcgc actttaattt tcgtgtgttg tacgcgccga atcaaccacg
     4321 ccaagtaagt gaatggcttg cctcgaatga agcgttccta ctggtggtga atggcggcta
     4381 cttcgatccc gcctatcacg cgctgggcca gttgatcagc gacggaatca gtgttggccg
     4441 taactatcaa ggcttcggcg gtatgttcgc agtaaacctt gatggcactg ttcaaatccg
     4501 atggaacatt cagcagccgt acgtcgaggg cgaaggtctg aagtatgcgc tgcaaaattt
     4561 tccgatgatg atcatccccg gcggcggatc gaacgatcag attgacgaca acggcgaact
     4621 cgcgctgcgc tcgatcgtcg gccaggatcg atcggggcgg atcatcttca tcgtcagtcc
     4681 cagcgccgcc ttcactttat cgggcatgag tcagtggctt ccgatcagcg atcttgatct
     4741 gaatgtcgcg ctcaatctcg acggtggtac at
//
LOCUS       DASRTJ010000642         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_363532,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000642 DASRTJ010000000
VERSION     DASRTJ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363532"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1208)
                     /locus_tag="VFF70_13040"
     CDS             complement(93..1208)
                     /locus_tag="VFF70_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZY45664.1"
                     /translation="MQKHELESQADRIEMVLRDHRAPARVTGGNVTPRWIQFLVQTAP
                     GIKVNRVEALSREIAMALGASNARVSMHAGAVRIEIPRSDPQPVKLLPLLARLPPIPA
                     GAALLGLADDGAPLLLRLPSTDVAHVLVAGTTGSGKTALVQSMIVSLAMSERRSQMQF
                     VLIDPKGRAFEGLSTLPHLLKPIVSQTDRAVQTLNELVSLMETRDQTRVTDPRIVIVL
                     DELADLVQTGGSEVIDRLGRLVQRGRQAGIHVIGATQKPSSAILGPLVKANFPVRLVG
                     RVVSSDDARVAAGIGGTGAERLTGHSDFVAVSGMGVTRFQAAFISPGEMLEVAAKLSN
                     GKHRSEIGRSDVKPAWGNDREESDVFRSALRVLRRVK"
     gene            complement(1330..1602)
                     /gene="rpsT"
                     /locus_tag="VFF70_13045"
     CDS             complement(1330..1602)
                     /gene="rpsT"
                     /locus_tag="VFF70_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZY45665.1"
                     /translation="MANIKSAIKRIRSSKRRAVRNKLVRTQAKSAVKEARGSMTSGDQ
                     KVIDASLSAASSALDKAAAKGVIHKRNAARRKGRLMKAAAKATTAK"
     gene            1703..2173
                     /locus_tag="VFF70_13050"
     CDS             1703..2173
                     /locus_tag="VFF70_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45666.1"
                     /translation="MSPDRSGRAPRWLKNILGHFYYWRGNMHRHFGNLSGERGEYESA
                     VNDFSRAINLKPDFISAHFNRGVLYWRELENFHHAIRDMSRVMELAPYRSEAWFNRAI
                     AYQLRGDSPQAIADLEYFLLIAGDDSWRASAESQLVAIKSLVEERAEIRRQRKT"
     gene            complement(2350..2970)
                     /gene="rimI"
                     /locus_tag="VFF70_13055"
     CDS             complement(2350..2970)
                     /gene="rimI"
                     /locus_tag="VFF70_13055"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZY45667.1"
                     /translation="MSNGWLPYRVDPMRWEDVPAVMTIERQSFTLPWSDYTYRHELLE
                     NIHSHYFIARRIDQTAAKRSSLFSRWRNNPKASVPIVGYGGFWLIADEAHISTIASHP
                     DWRGRGVGELMLLAMIERALQLNAFMITLEVRVTNSVAQSLYRKYGFEVVGQRPCYYR
                     DNNEDADLMTVEGLQKPEYRQKLQVLRIALEDQLRRDVKPEIVKAS"
     gene            complement(2967..>3660)
                     /gene="tsaB"
                     /locus_tag="VFF70_13060"
     CDS             complement(2967..>3660)
                     /gene="tsaB"
                     /locus_tag="VFF70_13060"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZY45668.1"
                     /translation="GLGWGFMLLAIDTSTRMASIAVYDGSIVLAEFTWQIGDHHTVEL
                     MPRLVDLLNQVSVSIDQLTGLAVALGPGSFTGVRVGVAAAKGLALARNIPIVGVRSSD
                     IVAYAQPPLEDRLVVVLQAGRGRLIVAHYDRDQNEWRQRGDFALTNARDLGQDWIKPT
                     LLCGELSASDREVITQRLGDRVRIVAPALSLRRAGYLAEIGWHIIRSGRHDDPAQLQP
                     IYVAPVVLEGAK"
BASE COUNT          872 a         1095 c          885 g          808 t
ORIGIN      
        1 cataacccac ctcgccagaa gccagtagca aaacatcgtt gaatttcaag tgttgccctc
       61 cagcttctaa cctccaattt ccatcatcta aatcacttca ctcttctcaa cacccgcagc
      121 gcacttctga agacatcact ctcttcacga tcattgcccc acgctggttt aacatccgat
      181 cgcccgatct cagatcgatg tttgccgttg ctcaacttcg ccgccacctc tagcatctca
      241 cccggcgaaa tgaaagccgc ctggaaacgc gtcacaccca tacccgaaac ggcgacaaag
      301 tcgctatgac cggtcaagcg ttctgcgccc gtcccgccga tacccgccgc cacgcgcgca
      361 tcgtcgctgc tgaccactcg accgacaagt ctcaccggaa aattcgcttt cactaacggc
      421 cccaggatcg cgctcgatgg tttctgcgtc gcaccgatca catgtatccc ggcttgtcgc
      481 ccacgttgaa ctaagcgccc tagtcgatcg atcacttcgc tgccaccggt ctgcaccaga
      541 tcagccagct catccagcac gatcacgata cgcggatcag tcacgcgcgt ttggtcgcgt
      601 gtctccatga gtgacaccaa ttcattcagc gtctgcaccg ctcgatcggt ttgagaaacg
      661 atcggtttca gtaaatgcgg caatgtgctc aagccttcaa acgcccggcc cttcggatcg
      721 atcaacacga actgcatttg cgatcgacgc tccgacatgg cgagtgaaac aatcatcgac
      781 tgcacgagcg ctgtcttgcc cgaaccggtg gtgcccgcga cgagcacatg cgcaacatca
      841 gttgacggca agcgtaataa cagcggcgca ccatcatcag ccaaacctag cagcgcagca
      901 cccgctggaa tcggcggtaa acgcgccagc aacggcagca acttcaccgg ctgtggatct
      961 gatcgcggaa tctcgatccg caccgcccca gcatgcatac tgacgcgcgc attcgacgcg
     1021 ccgagcgcca tggcaatttc tcgcgacagg gcttcaacac gattgacttt aatgcctggc
     1081 gcagtctgca ctaaaaattg aatccagcgt ggcgttacat tgccgccagt tacacgcgcc
     1141 ggcgctctgt gatcgcgcag caccatttca attcgatcgg cttgcgattc gagttcatgt
     1201 ttttgcatcg ggtaccccac aaaattatgc taaggttatt atatagaaca aatgttctaa
     1261 tgtcaagaga aagaaaatag ccgttgagtt tttccctcaa cgactattca atgaccggta
     1321 cgaacaaatt tacttcgcgg tggtggcttt ggcagcggct ttcatcaagc ggcctttacg
     1381 gcgtgcagca ttgcgcttgt gaatgacgcc cttcgctgcg gctttatcca acgcgctgga
     1441 tgctgcgctc agactggcgt cgatcacttt ttgatcacct gaagtcatac tgccccgcgc
     1501 ttctttcacg gccgacttag cctgtgtgcg caccagttta ttgcgcaccg cgcgccgctt
     1561 actgctgcga atacgcttga ttgctgattt gatattagcc aacggtgtcc tccaaacaat
     1621 ttcaacgacc caacggacgc agagtataca cgaactcgcg tgatttgtcc aacacgtttg
     1681 atcgaattat aatgcggtta acatgtcacc cgatcggtca ggccgcgctc cacgctggtt
     1741 aaaaaatata ttgggccatt tttattattg gcgcggcaat atgcatcgcc actttggtaa
     1801 cttaagcggc gagcgaggcg agtacgaatc cgccgtgaat gatttcagcc gcgcgatcaa
     1861 tctgaaaccc gatttcatct ccgctcattt caatcgcggt gtgttgtact ggcgtgaatt
     1921 ggaaaatttt catcacgcga ttcgggacat gagccgcgtg atggaattag cgccctatcg
     1981 cagtgaagcg tggttcaatc gagcaattgc ctatcagctg cgcggcgaca gtccacaggc
     2041 aatcgccgat ctggaatact ttctcttgat cgccggagat gattcgtggc gagcgagtgc
     2101 tgagagtcag ttagtagcaa ttaaatccct ggtggaagaa cgcgcggaaa ttcgtcggca
     2161 acgcaaaacc taagtatcca gagcggagat gtgaattcgc ggaagtcaca tcacagtcga
     2221 cgcctgccca tccccaactt tccagtggag aattggggat gggcaggacg cggtgtttga
     2281 aagaagagca agctgtgaaa tgcgcttctt cgactgcacc ttcatttgct acaccgacga
     2341 agtattcgct caggatgctt tcactatttc aggcttaaca tcacgccgaa gttgatcctc
     2401 cagcgcgatc cgcagcacct gtaacttctg gcgatattca ggcttttgca acccttcgac
     2461 cgtcatcaga tcagcgtcct cattgttatc gcggtaataa cacggccgct gtcccaccac
     2521 ttcaaatccg tactttcgat acaaactctg cgccacagaa ttagtcaccc gcacttcaag
     2581 cgtgatcatg aacgcattca actgcagcgc acgttcgatc atcgccagca gcattaactc
     2641 acccacaccg cgcccgcgcc aatcgggatg agaggcaatg gtgctaatat gcgcttcatc
     2701 ggcgatcagc cagaagccac cataccccac aatcgggacc gaagctttag gattgttgcg
     2761 ccagcgcgaa aacaaactcg atcgcttggc cgctgtctga tcgatccgcc gcgcgataaa
     2821 ataatgtgaa tggatattct ccagtagctc atgccgataa gtgtaatccg accacggcag
     2881 cgtgaacgat tgacgctcga tcgtcatgac ggctggcaca tcttcccagc gcatgggatc
     2941 gacgcgataa ggcaaccagc cgttactcac ttagctcctt caagaacaac aggcgcaacg
     3001 tagatcggct gcagctgcgc aggatcgtca tgccggcccg atcggattat atgccagccg
     3061 atctcggcca ggtaaccggc acgacgcagg ctcaatgctg gcgcaacgat gcgaacgcga
     3121 tcacccaatc tttgcgtgat cacttctcga tccgatgcag agagttcgcc gcacaataga
     3181 gttggcttga tccaatcttg tcccaagtca cgcgcgttgg tcagcgcaaa atcgccgcgt
     3241 tgacgccatt cattttgatc gcgatcatag tgcgcgacga tcaatcggcc gcggccggct
     3301 tgcagcacga caaccaatcg atcttcgagc ggcggttggg catacgccac aatgtccgac
     3361 gatcgtacac cgacgatggg aatgttacgc gccaatgcga gtcctttggc cgcggccacg
     3421 cccacgcgca cacccgtgaa cgatcccggt ccaagcgcga cagccaaacc agttaattga
     3481 tcgatcgata cactaacttg attgagcaag tcgacaaggc ggggcatcaa ttcgacggta
     3541 tgatgatcgc cgatttgcca ggtgaattcg gccaacacaa tcgatccgtc atagacagcg
     3601 atgctggcca ttcgagtgga tgtatcgatc gctaagagca tgaaccccca ccctaacccc
//
LOCUS       DASRTJ010000643         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_135918,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000643 DASRTJ010000000
VERSION     DASRTJ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135918"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1527
                     /locus_tag="VFF70_13065"
     CDS             <1..>1527
                     /locus_tag="VFF70_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45669.1"
                     /translation="LILARGFAYRAVADIPATIADYEAALNLARELHAVPRQVQSITR
                     LVEIYCNIGRATEARPRAEEALQLARGVADRKSEADSLEAVGATYIYISDYAHAKEYY
                     EQALTLYRELGDRAGEAQCLRRLGWVAGDLGDLSLARDYQHAALALYREIGDRNGEVR
                     ALNMLAISSLDYADQRAYEEEALDIARAIGSRDGQALMYGNLSAVYNALGLYSTARDY
                     VERGVQIYRELESKSYLANGVMGLADLHLIKGEYDQVEPLLTEARSLAEASDDPKTVG
                     TYWLQLGRLRLAQGQAPAAHAAFEKFDELAHQLKMPAYFTQAWTLMGAASLALGEVAA
                     AQHETSEAVRQLEALGTVVTDIQTQEIWWWRYQALKADKPKGKTKSAQLIDEAWQVLQ
                     KAYDAMLANIATLSDEGLRRNYLNKVKLNRDILIEWTRQAIDRTIPIETAVARSGNLQ
                     EQFRRLLSIGVRLNEPREVSALLDFIMDQLIELSGAERALLILVDQAGQRSIAASRGY
                     T"
BASE COUNT          335 a          394 c          477 g          321 t
ORIGIN      
        1 tctgatcttg gcgcggggct ttgcctatcg ggcggtggct gatattccag cgacgatcgc
       61 cgattacgaa gcagcgctga atctggcgcg tgaattgcac gctgtgccgc gccaggtgca
      121 atcgatcacg cgattagtcg aaatatactg caatatcggc cgcgccaccg aagcgcgacc
      181 acgggctgaa gaggcgttgc agttggcgcg cggcgtggcc gatcgcaaat ctgaagcgga
      241 tagtttggag gctgtgggcg cgacctacat ttatattagc gactatgcgc acgcgaaaga
      301 atactatgaa caagcgctga cgctctatcg tgaactgggc gatcgtgccg gtgaagcgca
      361 gtgtctgcgg cgcttgggtt gggtcgcggg tgacctgggt gatctctccc tcgccagaga
      421 ttatcaacac gccgcgctgg ctctgtatcg tgagatcggc gatcggaacg gtgaagtccg
      481 cgcgctgaat atgctggcga tctcttcatt agattatgcc gatcagcgcg cgtacgaaga
      541 agaagcgctg gacattgcgc gtgccatcgg ctcgcgcgac gggcaggcct tgatgtatgg
      601 caacctcagc gccgtgtata acgccctggg gttatacagc acggcgcgcg actatgttga
      661 acgcggcgtg cagatttatc gcgagctgga gtccaagtct tatttagcta atggcgttat
      721 gggcttggcc gatctgcact tgattaaagg cgaatacgat caagtggaac cgttgttgac
      781 tgaagcccgg agtttggcgg aggccagcga tgatcctaag acagtcggca cttattggct
      841 gcagctgggc cgtttacgct tagcccaagg gcaagctcca gcggcgcacg cggcttttga
      901 aaaattcgac gaactcgctc atcagttgaa aatgccggca tactttaccc aagcgtggac
      961 gctgatgggc gcggccagtt tggcgctggg tgaggtggcc gcggctcaac acgaaacgag
     1021 tgaggccgtt cgtcaactcg aagcgttggg gacggtggtc accgacattc agacgcagga
     1081 aatttggtgg tggcgctatc aagccttaaa ggcggacaaa cctaaaggca aaactaaatc
     1141 cgctcagttg atcgatgaag cctggcaggt gttgcagaaa gcctacgacg cgatgctggc
     1201 taacatcgcc acgttgagcg atgaaggcct gcgccgaaat tatttgaaca aggtcaaact
     1261 taatcgcgat attctgatcg agtggacgcg gcaggcgatc gatcgcacaa tcccgatcga
     1321 aacggccgtg gcccgatcgg gcaacttgca ggaacagttc cgtcgcttgt tgtcgatcgg
     1381 cgtgcgcctg aacgaaccgc gcgaagtcag tgctctgctc gacttcatca tggatcagtt
     1441 aattgagttg agcggcgcag aacgcgcgct gttgatcttg gtcgatcaag caggccaacg
     1501 atcgatcgcg gcctcacgcg gctacac
//
LOCUS       DASRTJ010000644         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_364039,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000644 DASRTJ010000000
VERSION     DASRTJ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364039"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFF70_13070"
     CDS             <1..346
                     /locus_tag="VFF70_13070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45670.1"
                     /translation="ETDLPDLMARCRAFVFPGDEDFGIAPVEAQASGRPVIAFGAGGA
                     LDTVLDGQTGVLFKEQNVDALIEAIQHLTSLKFDPQLARSNAEQFDSNVFKEKLGKFI
                     VEKYIEQSTRNA"
     gene            421..1098
                     /locus_tag="VFF70_13075"
     CDS             421..1098
                     /locus_tag="VFF70_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45671.1"
                     /translation="MELRLILKILLRRWWLVLLPPLIVAAYTLVTYLSPAPTFAATLR
                     FSVGYTPQSEGATLYDKFYPAWLTSEYIAGGLSDWAKTGNFAQHVADTASADGLILTA
                     DEAAGSIAASDHQRSIVLLYFSSGDQNKLLAIASASIKTMQSLNGIIFPQNGPSGATV
                     TPLDAISIAPVPPSLKSRIDIPVRIALGLALGIALAFIAHYFDPLIRDREEVEALGYK
                     IVGEIPK"
     gene            1195..1884
                     /locus_tag="VFF70_13080"
     CDS             1195..1884
                     /locus_tag="VFF70_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZY45672.1"
                     /translation="MELKDYLRILRKRGWIIILVIAMSAATAWGVSKLQTVEYKSSIQ
                     VFVRPERIDNGTLLAVSQILRGFVAYIDSRTFAQRVINTLGLDMLPETLISKVTIASI
                     NENYIIQIDVIDTNGDQANRIADEWANQFVLWRNQDNANQLKQDRVDANSVQNAAYVQ
                     FRPQTTLNTVAGGIIGLMVGALIVFFLESIESNILRAPDDVERALGITVLAAIPVTGA
                     DSGRSKRKSTG"
     gene            1964..2635
                     /locus_tag="VFF70_13085"
     CDS             1964..2635
                     /locus_tag="VFF70_13085"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZY45673.1"
                     /translation="MNQSLNSLITLSDPRSPAAEAFRTLRTNIYFSSLDRSIHTLVVT
                     SAAPGEGKSMVLANLAVAMAQGDTKTILVDADLRRPTVHTTFGLNNDAGLTSLFINAK
                     GPIEPALKDVGVNNLQVLTSGPLPPNPAEVLGSKRMLDVIADLKTRAEIVLFDAPPVI
                     AVTDASVLGSRVDGVLLVINSGHTRREHARRAMAQLEKLNIRVVGVVLNGTTIDNAMG
                     GYYSA"
     gene            2764..>3408
                     /locus_tag="VFF70_13090"
     CDS             2764..>3408
                     /locus_tag="VFF70_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZY45674.1"
                     /translation="MKGLILSGGKGTRLRPFTYTGAKQLVPLANKPVLFYAIEDLVES
                     GITSIGIVISPETGDQVKAAVGDGSKFGAQITYILQAAPKGIAHGIKIAQDFIGDDKF
                     VLFLGDNFLRDGIVSQVKAFSDAAMNAQIILYSMDDPSSMGVAVLDADGRVTKLVEKP
                     KQFISPYAVIGIYMFDRHVFEAVNNIKLSARGELEITETIQYLIDHGLNVRAHQL"
BASE COUNT          804 a          933 c          877 g          794 t
ORIGIN      
        1 tgaaaccgat ctgccggatc tcatggcgcg ctgtcgagcg tttgtatttc cgggtgatga
       61 agatttcggc attgcgcccg tcgaagcgca ggcgagcggt cgaccggtga ttgccttcgg
      121 cgcgggcggc gcgctcgaca cggtgctcga tggccagacc ggtgttttat tcaaagaaca
      181 aaacgttgat gcgctgatcg aagcaataca gcatctcact tcgctgaaat tcgatccgca
      241 actggcccga tcgaacgccg agcagtttga tagcaatgtt ttcaaagaga agttaggcaa
      301 attcattgtc gagaaataca ttgaacaatc aaccagaaat gcttaatgca taatgcacaa
      361 tttcattgtc acagcgttca ttgagcactg tgcattatga aatatgaatt ggagttaggt
      421 atggaacttc gtctcatctt gaaaattctt ttgcgccgtt ggtggctcgt tcttctgccg
      481 ccgctgatcg tagccgcgta tactttagta acataccttt cgcctgcgcc gacgtttgca
      541 gcgacgcttc gcttttcggt gggttatacg ccgcaaagcg aaggcgcaac gctgtacgat
      601 aaattctatc ccgcttggct gacttccgaa tacattgccg gcggcttgag cgattgggcc
      661 aaaaccggaa acttcgcgca gcacgtagcc gacaccgcct ccgccgatgg gctaatcctc
      721 accgccgatg aagccgccgg atcgatcgct gcgtccgatc atcagcgtag tatcgtcctg
      781 ctctatttca gcagcggcga tcaaaataaa ctgttggcca tcgccagtgc ctcgatcaaa
      841 acgatgcagt cactcaatgg catcatcttt ccgcagaacg gcccatcggg cgccacggtt
      901 accccgctgg atgccatctc gatcgcacct gtgccgcctt cactaaagag ccggatcgat
      961 attcctgtcc gcatcgcgct ggggctggcg ttggggatcg ctctcgcctt catcgcgcac
     1021 tacttcgatc cgctcattcg ggatcgagaa gaagttgaag cgctgggcta caagatcgtc
     1081 ggcgagattc caaaataatc gaagtccaag actattgtgt cattagtcat tcgtcatttc
     1141 tcatcggtct ggtatggtag aatgacgcgt gattctttag gagaacttta atcgatggag
     1201 ctgaaagatt atttacgaat attacgcaag cgcggctgga tcattattct ggtcattgcc
     1261 atgagcgccg ccacagcttg gggcgtcagc aagttgcaga cggtcgagta caagagttcg
     1321 atccaggtct ttgtccggcc agagcgcatt gacaacggca cactgctggc cgtcagtcaa
     1381 atcctacgcg gatttgtcgc atatatcgac agccggacct ttgcccaacg cgtgatcaat
     1441 acgctgggac tggatatgct gcccgaaacg cttatcagca aagtgaccat cgcttcgatc
     1501 aatgagaatt acatcattca aatcgacgta atcgatacca acggcgatca agccaaccgc
     1561 atcgccgacg aatgggccaa tcaatttgta ttgtggcgca atcaagacaa tgccaatcaa
     1621 ctcaaacaag atcgcgtcga tgccaatagt gtgcagaacg ccgcctacgt gcagttccga
     1681 ccgcagacca cgctgaacac agtggcgggc ggcattatcg gcctgatggt cggcgcattg
     1741 atcgtgttct tcctggaatc gatcgagtct aacatcctgc gcgcgccgga cgatgtcgaa
     1801 cgcgccttag gcatcaccgt tctggctgcc attccggtga cgggcgcgga ttcaggtcga
     1861 tcgaaaagaa aatctactgg atgattagtg actagtaact agtgcaagca aactactcat
     1921 cgctaattac tattcattga ccttcattac caaaggacgt tcaatgaacc aatctctcaa
     1981 ttcactcatt accttatccg atccgcgctc accagcggcc gaagccttcc gcacgctgcg
     2041 cacgaatatt tatttctcca gcctggatcg atcgatccac acgctggtcg ttacctcggc
     2101 cgcgccaggc gaaggcaaat cgatggtgct ggcgaatctc gcagtggcga tggcacaggg
     2161 cgataccaaa acaatcttag tcgatgccga tctgcgccgc ccgacggttc acacaacgtt
     2221 tggcttgaat aacgatgcgg gactcacctc actcttcatc aatgccaaag gcccgatcga
     2281 gccggcgctg aaggatgtcg gcgtgaacaa cttgcaagtc ttgaccagcg gccccctgcc
     2341 accgaatccc gccgaagtcc tgggctcgaa gcgcatgctg gatgtgattg cggatctgaa
     2401 gactcgcgcc gagatcgtcc tgttcgatgc gccgccggtg attgcggtga cggatgcgtc
     2461 cgtcctcggc agccgcgtcg atggggtgct gttggtgatc aactccggcc acacgcgccg
     2521 cgaacacgcc agacgcgcga tggcgcaatt ggagaaactt aacatccgcg tcgtcggtgt
     2581 ggtgttgaac ggcacaacga tcgataatgc aatgggcggc tattattctg cctagaagtt
     2641 tttataccgc aaagaacgcg aagagcgcaa agtatattta tttttttctt cgcgaacttt
     2701 gcgcccgctg cggttcaata caatttccaa catccaacct ccaacttcta aaatggagca
     2761 ctcatgaaag gtctcatcct ctccggcggc aaaggcacac gcctgcgccc cttcacttat
     2821 accggcgcga agcagctcgt tccattagcg aataagcctg tcttgttcta cgcgatcgaa
     2881 gatctcgtcg agtcgggcat cacttcgatc gggattgtca ttagtcctga gacaggcgat
     2941 caagtcaaag ccgccgtcgg cgatggatcg aagttcggcg cgcagattac ttacatcttg
     3001 caggctgcgc cgaaagggat tgcccacggc atcaagatcg ctcaagactt catcggcgac
     3061 gataagtttg tcttgtttct cggcgataac tttttgcgcg atggcatcgt atcgcaagtc
     3121 aaagccttta gcgatgcggc gatgaatgca caaatcatcc tgtattcgat ggacgatccc
     3181 agttcaatgg gcgtcgcggt gctcgacgcg gatggccgcg tgacaaaatt agttgagaaa
     3241 ccgaagcagt tcatctcgcc atatgccgtc atcggaattt atatgtttga tcggcatgtg
     3301 tttgaggccg tgaacaacat caagctatcg gcgcgcggcg aactcgaaat caccgaaacg
     3361 attcaatacc tgatcgatca cggcttgaac gtgcgcgcgc atcaactc
//
LOCUS       DASRTJ010000645         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_364043,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000645 DASRTJ010000000
VERSION     DASRTJ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364043"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VFF70_13095"
     CDS             complement(<1..1632)
                     /locus_tag="VFF70_13095"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZY45675.1"
                     /translation="MTNRTLKIGGALLALVMLIIIGAIVFINNLPEVADQLKSNASPV
                     IVTLTQPLNGSTWERNLSIPIKASASPFTSVASIELWVDGTPTETLTSKTGSAEWVAV
                     LQTEGEHTFFVRAIDKQSRVANSNVVRVIASHTTDPGATLLQPVKGGDTVASLAANFN
                     TTPQDIIDLNPGLDPNSALPPGNPIKIPIQPPALSLVNNPTSPVPSGPTQPGSNKPPG
                     KFAFWVSQFFSPKAPPAPDLALQVNQCAVNLFITPKSKLSQSTNVYRFDAATNVFIKI
                     ATLADSNGNPIGYSDPDRNGNVLYYVSAFNAAGESPSNIVSADITDPVCLTPFWIGVK
                     FSQTPLKLKQSVGLNPDIIPTKYDQAYFYLSINNSGSIRVPADPNAFITHMPDGYFIP
                     DEQIKNFVKPAAEDLAVEIDAWGWSAGQLLHIGTFQRTVPALKQNTTLPADYTASQLL
                     LCDIPTSHCEKEGLSSYVTEAYSSDYLGWGFRWLPDPSNADHGLWQVSVSAFPNDCAV
                     NPPDLIATGQIDAASGHYSFFFIDFALLKDKLPNVG"
BASE COUNT          373 a          406 c          482 g          381 t
ORIGIN      
        1 gcctacgttt ggcaacttgt ctttcaagag cgcaaagtcg atgaagaaga aagaatagtg
       61 cccactcgcc gcatcgatct gacctgttgc aatgagatcg ggcggattaa cagcgcagtc
      121 gttgggaaaa gcgctgacgg aaacttgcca cagaccgtga tcggcgttcg acggatcggg
      181 cagccaacgg aacccccagc ccaagtaatc ggaggagtaa gcttcggtga catagctgct
      241 caatccttct ttttcacaat ggctagtcgg aatatcacac aatagcaatt ggctggccgt
      301 ataatcggcg ggcagcgtcg tattctgttt gagggccggc accgttcgct ggaaagttcc
      361 aatgtgcagc agttgtccgg cactccagcc ccacgcatcg atctccactg ctaaatcttc
      421 ggcagcgggc ttaacaaaat ttttgatctg ctcgtcgggg atgaagtaac catcgggcat
      481 gtgggtgata aacgcattcg gatcggccgg cacgcggatc gatccactgt tgttgatcga
      541 tagataaaaa taggcttgat catacttcgt tggaataata tctggattca gcccgaccga
      601 ttgttttagt ttgagcgggg tctggctaaa cttgacgccg atccaaaacg gcgtcaggca
      661 aactggatca gtaatgtcgg cgctgacgat attgctgggc gattcaccgg ccgcattgaa
      721 ggcggacaca tagtacagca cattgccatt ccgatcggga tcgctatacc cgatcgggtt
      781 gccgttgcta tcggctagag tcgcgatctt aatgaagaca ttggtcgccg catcgaagcg
      841 atagacgttg gtgctttgcg agagtttcga tttcggcgtg atgaacaagt tgaccgcgca
      901 ttgattgacc tgcaaggcta gatcaggcgc gggcggcgct ttgggcgaaa agaactgact
      961 gacccaaaat gcgaatttgc ccggcggctt gttcgatccg ggttgagttg gcccactcgg
     1021 cacgggcgat gtcggattgt tcacaagcga taaagcgggc ggttggatcg gaatcttgat
     1081 cggattgccg ggcggcagcg cggaattggg atcgaggccg ggattcaaat caataatatc
     1141 ttgcggcgtg gtattgaaat tcgcggcgag acttgcgacg gtgtcaccgc ccttgaccgg
     1201 ttgaagcagg gtagcacccg gatcagttgt gtgtgaagca atgacacgca cgacgttcga
     1261 gttagcgacg cggctttgct tatcgatcgc gcggacgaag aatgtatgct cgccttcggt
     1321 ctgaagcaca gcaacccact ctgctgatcc agtcttcgac gttagcgttt ccgtcggcgt
     1381 gccatcgacc cacaattcga ttgaagccac cgaagtgaat ggtgaggccg atgccttgat
     1441 cgggatcgac agattgcgtt cccaggtgct gccgttcaac ggctgggtca gcgtgacgat
     1501 caccgggctg gcattcgatt ttagttggtc ggctacttcg ggtaaattgt taatgaagac
     1561 gatcgcgccg atgatgatca gcatgacgag cgcgagaagt gcgccgccaa tcttgagagt
     1621 tctattcgtc atgtcccacc cc
//
LOCUS       DASRTJ010000646         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_136408,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000646 DASRTJ010000000
VERSION     DASRTJ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136408"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1008)
                     /locus_tag="VFF70_13100"
     CDS             complement(241..1008)
                     /locus_tag="VFF70_13100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45676.1"
                     /translation="MIDRDHIRIFLETDREWSAYALGDLDPELYQECEWFGAEDNGRL
                     RSIVLLFKGLEPPVIFTIGEASGIDQILDRLMHARSVYLSIREDHLRAIETQYHLGRR
                     ESMWRMILKPSEFRSIEGEAIDLQLADIDDLTCLYALGGGDAFTRSQLSSGVFYGLRK
                     KDRIAAVAGTHVVSIEQSVAAVGNIMTHPDDRHHGYASIVTSAVCAELIRRGIRTIAL
                     NVSQSNLAAIRVYEKLGFKKYVHFYEGIALRKETKRS"
     gene            complement(1079..2035)
                     /locus_tag="VFF70_13105"
     CDS             complement(1079..2035)
                     /locus_tag="VFF70_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZY45677.1"
                     /translation="MNIVVTGSIAYDYIMSFPGRFRDHILPEQLDNLSLSFLVDSMKK
                     QRGGTAANIAYNLALLGERPCVMATAGQDFGEYRVWLEQHGVDTSAIKQYDDEFTSSF
                     FVSTDLDQKQIANFYTGAMARASELSFKQLNSAKPDLVIISPNDPQAMKAYALECKAL
                     DIPYIYDPSQQIVRLSDEDLRQGIDGSRMLIVNDYEFEMLRGRLHLAESDVLQRTQTL
                     IITRGENGSTIATPTDRVDVPPVLTEHQVDPTGVGDGYRAGLMKGMALGLPWEIAGRM
                     GALAATFVLEHMGPQGHTYTHAEFVQRYRAHFNDSGALDKWE"
     gene            complement(2173..2868)
                     /locus_tag="VFF70_13110"
     CDS             complement(2173..2868)
                     /locus_tag="VFF70_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45678.1"
                     /translation="MPKLSIIICMYNEKASALPVIDKVRSVSLSAGWSKEIIVVDNCS
                     IDGTRELLQQLPYNDVRVIYQPRNLGKGASIRTGFREAIGEYGVIQDADFEYDPAELS
                     MLTAKATEAGVVAVFGSRTAGGRHIYKYKQNYWGVKVLTSITNILFGGHLTDVAVATK
                     MVKRSVFQSLDLKGSSFDLDFELPCKLLKRKHSIAEVPVSYKPRTIAEGKKIRWTDGL
                     HALAVIFRERFTK"
     gene            complement(2928..>3346)
                     /locus_tag="VFF70_13115"
     CDS             complement(2928..>3346)
                     /locus_tag="VFF70_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZY45679.1"
                     /translation="QLDKLPTFIEKRKKHFRMLFDHLQQYQDYLQLPTWSPKADPAWF
                     AFPITVRSDAPFNRIALTKFLEAHRIETRSLFAGNILSQPGYRNIERRVIGELPNADI
                     VLRRTFFVGVYPGLDEQQIGYMLATFDKFFNLMHSA"
BASE COUNT          810 a          933 c          801 g          802 t
ORIGIN      
        1 tcgcaacacc ggcatctcct gctcggccca ctcgatacgg taacgaccac ctgtggctaa
       61 atctaatttc ttaacgtgat gttcgactgt catcgcctct cctatttctg agtagtgatt
      121 tcatttgtca ttctgagcga agcgaagaat ctctgcgcca atcgatagag accctgccca
      181 tactctgagc gcatcgcgct aagtggggcc gcaaggcgta gcctttgctc agggtgacaa
      241 tcaagatcgt tttgtttctt ttctcaacgc aataccttcg taaaaatgca cgtacttctt
      301 aaagcccaac ttctcgtata cacgaatcgc ggctagattc gattgagaga cattcaaggc
      361 gatggttcga atcccgcgcc ggatcaattc agcgcatacg gcgctggtca cgatcgatgc
      421 ataaccgtga tggcgatcat cgggatgagt catgatgttg ccaacggcgg ccacactctg
      481 ttcgatcgag accacatgcg ttcctgctac ggcggcaatt cgatcttttt tcctcagccc
      541 gtagaacact ccgctgctta attgagatcg agtaaacgca tcaccgccgc cgagcgcgta
      601 taaacaagtt agatcgtcga tgtccgccaa ctgtagatcg atcgcttccc cttcgatcga
      661 tctaaattca ctcggcttca agatcatgcg ccacatcgat tctcgccgac cgagatgata
      721 ctgcgtttcg atcgcgcgca aatgatcttc gcgaatacta agataaaccg atcgggcatg
      781 catcaaccga tcgagaatct gatcgatccc gctggcctcg ccgatcgtaa agatcacggg
      841 cggctcaagt cctttgaaca gcaacacgat cgatcttaat cgaccattgt cttcagcgcc
      901 gaaccattca cattcttggt aaagttcagg atcgagatcg cccagcgcat aggccgacca
      961 ctctcgatcg gtttcgagaa aaatgcgaat gtgatctcga tcaatcaagc gtgttacttg
     1021 catcgtagcg aataacgagt gatgagtaag ttactcgtta ctcattgctc gttaagcctc
     1081 actcccactt atccagcgca ccactgtcat taaaatgtgc gcgataacgc tgcacgaatt
     1141 cggcgtgcgt ataggtgtgg ccctgcggac ccatatgctc caacacaaat gtggcggcga
     1201 gcgcgcccat gcgaccggcg atctcccacg gcaagcccag cgccatgcct ttcatgagtc
     1261 cggcgcgata gccatcgccc acacccgtcg gatcgacttg gtgctcggtc aggacaggcg
     1321 gcacatcgac gcgatccgtt ggcgtggcaa tcgtcgagcc gttttcgccg cgagtgataa
     1381 tcaacgtctg cgtgcgctgg agaacatcgc tttcagctaa atgcaatcga ccgcgcagca
     1441 tctcgaactc ataatcgttg acgatcagca tgcggctgcc gtcgatgccc tgccgcaaat
     1501 cttcgtcgct cagccggaca atctgctgac tgggatcgta gatataggga atgtccaacg
     1561 ccttgcattc aagcgcgtaa gccttcatcg cttgcggatc attcggtgag ataatcacga
     1621 gatccggttt ggcggaattc aattgcttga acgagagttc gctggcccgc gccatggcgc
     1681 ctgtataaaa attagcgatc tgcttctgat ctagatcggt gctgacaaag aacgaactgg
     1741 taaattcgtc atcgtattgt ttgatggccg acgtatcgac gccatgctgt tccagccaca
     1801 ctcgatattc accaaaatcc tggccggccg tcgccatcac gcacggccgc tcgcccaaca
     1861 aggcgagatt gtatgcgatg ttcgccgccg tgccaccgcg ctgcttcttc atcgaatcga
     1921 cgaggaaact caagctgaga ttatccaact gctccggcaa tatgtgatcg cggaagcgtc
     1981 ccggaaagga catgatgtaa tcgtaggcga tcgatccagt gacaacgata ttcatggttc
     2041 tcccgatgct cgtcattgcg aggaagccgt aggccgacgc agcaatctcc ttaggttgtc
     2101 attgagattg cttcgccgcc cgaaggcctt gcctgctctg tgcagggttg cggctcgcaa
     2161 tgacgttgta tgttactttg taaaccgttc acgaaaaatg actgccagcg catgcaggcc
     2221 atccgtccaa cgaatctttt tgccttcagc aatcgtgcgc ggtttgtatg acaccggcac
     2281 ttcagcgatc gagtgtttac gcttcagcaa cttacatggc agttcaaaat ccagatcgaa
     2341 cgacgagcct ttcagatcga gcgattgaaa cacactgcgc ttgaccatct tagtggcaac
     2401 cgccacatcg gtgagatggc cgccgaataa aatattggta atacttgtca gaactttcac
     2461 gccccaatag ttctgcttgt atttgtaaat atgccgcccg cccgccgtgc gtgatccaaa
     2521 tacggccacg acaccggctt cagttgcctt cgccgtcagc atggacaact ctgctggatc
     2581 atattcgaag tccgcatcct gaatcacgcc atactcaccg atcgcttcgc ggaaacctgt
     2641 gcggatcgat gcgcctttgc ccaagttgcg cggctgataa atcacacgca catcgttata
     2701 cggcaattgc tgcaataact cgcgcgtgcc gtcgatcgaa caattatcga cgacgatgat
     2761 ctctttcgac cagcccgcgc tcagcgaaac cgatcgaact ttatcgatga ccggcagcgc
     2821 acttgctttt tcattgtaca tgcagatgat gatcgacagc ttaggcaaga atgattccta
     2881 atgagtgcat aattctcaat gcacaatgca taattgagaa ttgtgaatta tgcactatgc
     2941 attaaattaa aaaatttatc aaacgtcgcc agcatataac cgatctgctg ctcgtctaag
     3001 cccggataca cgccgacgaa aaaagtccgg cgcaaaacaa tatctgcatt gggcagctca
     3061 ccgatcactc ttcgctcaat attgcgatag cccggctggc tcaagatatt acccgcaaac
     3121 aacgatcgcg tttcgatgcg gtgtgcttcc aagaatttgg tgagcgcaat ccgattgaac
     3181 ggcgcatccg atcgaactgt gatcggaaac gcaaaccagg ccggatccgc tttgggcgac
     3241 cacgtgggca gttgcagata atcttgatac tgctgtagat gatcgaacaa catgcggaaa
     3301 tgcttcttcc gtttctcaat gaacgttggc agcttatcga gctgcg
//
LOCUS       DASRTJ010000647         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_289887,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000647 DASRTJ010000000
VERSION     DASRTJ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289887"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFF70_13120"
     CDS             <1..721
                     /locus_tag="VFF70_13120"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZY45680.1"
                     /translation="VRGPSHPNYLMLMAAQSPIVNTPFPSDVCPAFCLDLPTIVDRLD
                     AAQVSWRDYAGMFTDIKNLVGRKEITEYDDTPFFDDAAAGILPNVAWLNSGFLTNGGE
                     KSGHPTGSICEAQNYAVRVLNAIMHGPQWNSTAVILVWDDWGGFFDHVEPPTVETLAD
                     GTPFRYGFRVPCLVISPFAKHGYVSHQLHSHVSTLRFIESIFDLDPLTQRDAQASDLL
                     DCFDFAQAPRPPIDLVERVCG"
     gene            complement(858..2225)
                     /locus_tag="VFF70_13125"
     CDS             complement(858..2225)
                     /locus_tag="VFF70_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45681.1"
                     /translation="MSMPPAAPTPPPSNFTSANTGSRFKSAWDDPARRPSLVIGLGAV
                     ALALILVIVAIGQISGTGSTVTPTATENPCFINSNCPQGSIVPKILHVRGRSILIDPV
                     SMNNGQWKPSAAAGRAEWVYGTLINYLIGLSSSQENSDMLKALGQADEISLDLSNGQS
                     LKYKFAGRQLVTANASDIFAQSKPGLTLVLLGENSDQRIVITADYVAEQEVGKPAPGS
                     YAQINTPIVIGAAKVTVLSARLVINAPGIQVGSSFYMVDFSVENIGTTPLDAAGFLIQ
                     LQDYAKQKYNLSAAASALGPNPAPKGTLQPGTLATFTSGFEVPANVTGPVLIWVFLPQ
                     ANFTVQANVAVPLIGPTPTPDPKSKVVVQITQAYYTPDQSELIVVGGIGNPSSVPVTL
                     NTADISLSTPEGVLAKLQGAEPALPLNLAPGQNQTFTLHFARVPGSSATLKILFASFQ
                     LNGLQ"
     gene            complement(2274..>3040)
                     /locus_tag="VFF70_13130"
     CDS             complement(2274..>3040)
                     /locus_tag="VFF70_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45682.1"
                     /translation="TYDDGSWDNDFFKVRVKPGMLITCKTSALSAGTDTNLILYDESH
                     NGINGSDDLNLAGGDLSSAVTYYVTYEGWLYALVGQGFNVPQNLEASYTYNFECAIGN
                     LSTDTPTPTNTPAGPVEPTRTRIPTDTPTPTITPTPTPPFIQIQPLPTATPVGLPLTR
                     IPISLQVYYDADGNNKQDPGEGVSGVSVRIFDLVTGNLLTQGFTDETGRATFTVSAPG
                     AVHLVVPYFNFSTIVLPSGGSAVIRISPRELPQSIP"
BASE COUNT          643 a          829 c          878 g          690 t
ORIGIN      
        1 agtacgcggc ccgtctcatc cgaattattt gatgttgatg gccgcgcaat ccccgatcgt
       61 gaatacgccc ttcccgtcgg acgtgtgtcc cgctttctgt ctggacctgc cgacgattgt
      121 cgatcgactc gacgcggcgc aggtgtcgtg gcgcgattac gccggaatgt tcacggacat
      181 caaaaattta gtcgggcgca aagagatcac cgaatatgat gatacgccgt tcttcgatga
      241 tgcggcggcg ggaatcttgc cgaatgtggc gtggctcaat tccggttttc ttaccaatgg
      301 aggcgagaag agcggccatc cgaccgggag tatctgcgag gcccaaaatt atgcggtacg
      361 tgttttgaat gcgatcatgc acggcccgca gtggaattcg accgcggtca ttttagtgtg
      421 ggacgattgg ggcggcttct tcgatcacgt cgaaccgccg actgtggaaa cgctggcgga
      481 tggcacgccg tttcgatatg gttttcgcgt accgtgtctc gtgatcagcc cgttcgctaa
      541 acacggctac gtgtcacatc aactgcattc gcatgtcagc acgctgcgct tcatcgaatc
      601 gatcttcgat ctcgatccct tgacgcaacg cgatgcgcag gccagcgact tgctcgattg
      661 tttcgacttt gcccaggcgc cacgtccgcc gatcgatctg gtggagcgcg tgtgtggcta
      721 agaggtgact catcgagatt gaagttggca aggtgcaact gcatagaaaa tctcgggtat
      781 gttagcccgg gaccgatcga aaaaaagacc ggcgatcgat atcgatcgcc ggtcttgtcg
      841 tgggtctact tgttcagtta ttgcaagccg ttcaattgaa aggaggcgaa taatattttc
      901 aaggtcgccg atgagccggg cactcgcgcg aaatgcagcg tgaatgtttg attttgcccc
      961 ggcgctaaat tcaacggcaa ggccggctcc gcgccttgca attttgccag cacaccttcc
     1021 ggcgtgctca ggctgatgtc cgccgtgttc agcgtgaccg gcacgctcga tggattgccg
     1081 atgccgccaa cgacgatcaa ctcagattga tcgggcgtgt agtaggcttg cgtgatctgc
     1141 accaccactt tcgatttggg atcgggcgtc ggcgtcgggc cgatcagcgg cacagccaca
     1201 ttggcctgca cggtgaaatt tgcttgcggc aaaaagaccc agatcaacac cggcccggtc
     1261 acgttcgcgg gcacttcgaa cccggaggtg aacgttgcca acgtgccggg ttgcaaggtc
     1321 cctttaggcg caggattcgg gcccaaagcg ctggccgcgg ctgataaatt atacttctgc
     1381 ttggcgtagt cctgcagttg aatcaggaaa ccggcggcgt ccagcggtgt ggtgccgatg
     1441 ttttccaccg agaagtcaac catgtagaac gaactgccga cctgaatgcc cggagcgttg
     1501 atcaccaatc gggcggacag caccgtcact ttcgcagcgc caatcacgat cggcgtgttg
     1561 atctgcgcgt acgatcccgg cgcaggcttg cccacttctt gttcggctac atagtcggcc
     1621 gtgatgacga tccgctgatc ggaattttcg ccgagcagca ccaacgtcaa gcccggtttg
     1681 gattgcgcaa agatgtcgga agcattcgcc gttaccaact gccgaccagc aaatttgtat
     1741 ttcagcgact ggccgttcga tagatcgagc gagatctcat cggcctggcc gagtgctttc
     1801 aacatatcgc tgttttcttg cgaggacgac aagccgatca aataattgat cagcgtacca
     1861 tatacccatt cggcccggcc cgcggctgca ctcggcttcc attgcccgtt attcattgag
     1921 actggatcga ttaagatcga tcggccgcgc acatgcaata ttttcggaac aattgaacct
     1981 tgtggacagt tgctgttaat gaagcaaggg ttttccgttg cagtcggtgt aaccgttgag
     2041 cccgtgccgg atatttgacc gatcgccacg atcaccagaa tcaacgcgag cgcgactgca
     2101 cccaagccga tcaccaacga aggccgtcgc gcgggatcat cccacgcact cttaaagcgg
     2161 ctgccggtat tcgctgaggt aaaattggag ggcggcggtg tgggtgcggc cggtggcatg
     2221 gacatccggt tgaatctcct gagcgtgatg atgactccgc gtggagcgcg tcgttacgga
     2281 atagactggg gaagttcgcg cgggctgatg cgaatcacag ccgatccgcc ggacggcaaa
     2341 acgatcgtgc tgaaattgaa gtacggcacc accagatgaa ctgcgcccgg cgcgctgacc
     2401 gtgaaggttg cccgtccggt ttcatcggta aagccttgcg tcaacaggtt acctgtcacc
     2461 aaatcgaata tccgcacgga aacaccactg acaccctcgc ccggatcttg tttgttgttg
     2521 ccatccgcat cgtaatagac ttgcaggctg atcggaattc gcgtcagcgg caagccgact
     2581 ggcgttgccg tgggcagcgg ctgaatttga atgaagggcg gcgtgggggt cggtgtaatg
     2641 gtcggcgttg gagtatccgt tgggatacgc gttcgcgtcg gctcgaccgg gcctgctggc
     2701 gtattcgtcg gcgtgggcgt atccgttgag agattgccga tggcgcactc aaagttatag
     2761 gtgtagctgg cttccaaatt ctgtggcacg ttaaagcctt gaccgaccag cgcatatagc
     2821 cagccctcgt aagttacata gtaagtaacc gcactgctca gatcgccacc cgccagattg
     2881 aggtcatcac tgccattgat gccgttgtga ctctcgtcat aaagaatcaa attggtatcg
     2941 gtacccgccg acaaggcact ggtcttgcac gtgatcagca taccgggttt aaccctgact
     3001 ttaaaaaagt cattatccca agagccatca tcataggtac
//
LOCUS       DASRTJ010000648        11243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_289896,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000648 DASRTJ010000000
VERSION     DASRTJ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289896"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VFF70_13135"
     CDS             <1..1253
                     /locus_tag="VFF70_13135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45683.1"
                     /translation="VAARLLAVLIMGNQIQTLPGIDDQRSYDMLAQQVVTGHGFTVVT
                     DWWPLTRAGQPTAHWSYLYTLYLAFTYMIFGVQPIVARLLQAIIAGLFMPWLNFRLGR
                     RLANDRVGLMAAAWSAIYGYFVYYSAALMTEMFYITAILWSLDLALSMANNDRSIKRW
                     AVLGLSMGITALLRQTFLLIIPIVLIAALGLRVSDFKLKTAQSKLTAGLKGSIVALMV
                     MAALILPWTLRNYQAFNRFVLLNTNAGFAFFWSNHPIYGTEFVGILPRGTSYQSLIPP
                     ELRTLDEASLDAELMKAGINFVVQDPGRYALLSLSRFKTYFEFWPTSESELLSNVVRV
                     LSFGAALPFMLYGLILALRHWRQWLLLYVFAIVYSLIHLLSWALIRYRLPVDAVLIIF
                     AAYGLVDLIERSAPRLKLKPPNLA"
     gene            1253..2473
                     /locus_tag="VFF70_13140"
     CDS             1253..2473
                     /locus_tag="VFF70_13140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45684.1"
                     /translation="MEILFIVPYTPTLIRVRPYNLIKFLAQRGHQLTLATLWETADEF
                     RALAEWRSMGVDVLAARLTRARSILNALRTVPTRDPLQAAYCDSPQLRQLIQSKVNSQ
                     SFDAIHVEHLRGARYGLQLQLLTSNFKHPTPFIWDSVDCITYLFEQAARYRSGLFSWL
                     ITRLELGRTRKYEARMAAQFDRVLVTSEIDRQALAALSNRSTIDVLPNGVDLDYFTPR
                     LDRREADTVVFSGKMSYHANAAAARYLIDLIMPLVWRQRPTAKLIIAGSRPARSIRHA
                     GAVDARIEVTGSVEDMRVALRRASVSVAPLLYGAGIQNKILEAMACGVPVVASSRALA
                     ALPRVKRTSAVLVADEPRSFADAILRVLNDGELRAQLSAAGRQVVEAEHHWDHIAGQL
                     EVIYRAAIEQRLAK"
     gene            2585..3160
                     /locus_tag="VFF70_13145"
     CDS             2585..3160
                     /locus_tag="VFF70_13145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZY45685.1"
                     /translation="MDFTGSWILLLVLIPLLVILALIVKLDSHGPILHRRRVLGRNGT
                     EFDAFKFRTMQLNGHELLAAQPELKAALAAEHKIKNDPRLTRSGRWLRKLSLDELPQL
                     LNVLRGQMSLVGPRMIAPIELSNYGSFAAELLTVKPGLTGVWQLSGRSDLSYADRVQL
                     DIRYIRTRSIWIDLRLLLKTPWVVITGRGAY"
     gene            3162..4178
                     /locus_tag="VFF70_13150"
     CDS             3162..4178
                     /locus_tag="VFF70_13150"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HZY45686.1"
                     /translation="MLIARAPVRVSFFGGGTDLPAYYLQYGGSVLSTTINKYVYVIMN
                     VSEQNVLQITSSDYQTFYRHPPGEPLLWDGDLSLPRAVLHHFGVEHGVSLFLASEVPP
                     GTGLGSSSSVMVALIKAVSAARGMKLSKAQVAEMAVQIELEKMGNPIGVQDQYAAAFG
                     GLNCFTFDSAGIHAEPIDLPATTLKQLESNLLLIFTGSARDSAEILSRQTQASQQRDP
                     IVIEALQAVHELSRRARDYLVNSEVDQFGALLHEAWQHKKRFAAGVTNARIDQVYELA
                     LRHGALGGKITGAGGGGFLMLYCPADTIERVAGALADEGLRRMEFRFEQDGARVLFNA
                     GLRL"
     gene            4217..5752
                     /locus_tag="VFF70_13155"
     CDS             4217..5752
                     /locus_tag="VFF70_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZY45687.1"
                     /translation="MADHKHTLNRSTQTAYHRQWQALIAALIIFDALMIWGSLRLAYD
                     LRFTSSLLTYYGELSPATYAIMEMIGLPIWLGLFALAGLYQRKNLLGGTLEYKQVIKA
                     GSAGVVAIVSLAFVWRDVALVSRGWLVFAWIFLCASVSVERFVIRRVAYRLRRRGWLT
                     ARVLMVGANDQGIAIAKQWQQSHTSGMHVVGFVDDFKPIGTSVLDDLKVIGQPTALLQ
                     IIDQSGVDEVVVVPNAVAWETFEEIITQAGAVKDYELWLSPGFYEMLTNSVVVTNKSY
                     VPLFTINAARLVGPDAIMKAVLDYGLGFGLLLVMLPIMSLIAAGLKLSRRSVFMAHRT
                     IGQGDTIFLMWKFNSADDKPTRLQRGLHRYALDKLPQLFNVLTGHMSLVGPRPRVVDD
                     QSLDPRQVHNLRSVKPGLIGPWLVTEVWSSLDETRDDLYYVRNWTIWLDLQILFQSIV
                     AWFRLRRPMLMAESDTSRSEEAVLTDDTFEARRSISGLMRLPIKHGAPLLMDKDRSSQ
                     RLE"
     gene            5793..7124
                     /locus_tag="VFF70_13160"
     CDS             5793..7124
                     /locus_tag="VFF70_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZY45688.1"
                     /translation="MLEEYLDRYLDDIASALKKLDRRAVWAVIEELMTAWREHRQVFL
                     LGNGGSAALASHMANDLCKTSIVNGQPRLRAIALTENVPLLTAWANDNGFEHIFSEQL
                     YNLCQPNDLVIAISCSGNSTNVLRALRAARELRARTIGFTGDTGGQLKDLVDLCVFAP
                     AEKIWPQEDVHVILDHVIASTLHLWITAIAEQSAHPMRGMILAAGAGTRLRPLTLDKP
                     KPMLPINGQPLLHYTIEWLREAGIHEVAINVHHRPDAIIDHFGDGSRWGVKVHYSHED
                     QLWGTAGAVRRLNGFIDNDALVVVYGDVLTNMDLRGLMAFHRQQLARDPSLGVTLSLQ
                     HLPNPIGKGLVELDDMGRVTRFVEKPAAHDMFGDLVNAGVMIVEPSVIDRIPRDTFYD
                     FGQHLLPELLHSDRSIYGWIVPPQTYLIDIGTPENYAQAQRDWPVSAIDRA"
     gene            7094..7699
                     /gene="gmhB"
                     /locus_tag="VFF70_13165"
     CDS             7094..7699
                     /gene="gmhB"
                     /locus_tag="VFF70_13165"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZY45689.1"
                     /translation="MAGVRDRSGLIMRPAIFLDRDGVINRNRNDYIKAWSEFEFLPGS
                     LNALQRLASLEWPIVVITNQSAVGQGLISRAQVDEIHRQMLQAISAAGGRINAVLYCP
                     HRADEGCTCRKPHPGLILQVAEHLAIDLPHSFFIGDAENDILAARAAGCQPILVKTGR
                     GVQEWTILRQHQVSGFQVAEDLGEVADWLCQLAATDFGAAE"
     gene            7789..9465
                     /locus_tag="VFF70_13170"
     CDS             7789..9465
                     /locus_tag="VFF70_13170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZY45690.1"
                     /translation="MELRQYLNIVRKWWWLIVISMVIAGGAGYFATKSIPPTYLSRVT
                     LMVGQALQNPNPSQNEIFTGEALAQSYADLVKRQPVLQGTLNALSLNWELTALQQMVS
                     SRVVLGTQLLEITVLDTDPNRAQTLANEIGHQAILLSPSSTDLGEADQRQFILDQLVD
                     LKANIQHALTENAQLDQTITSTGSASQVQAARDRQAVLRTQISSWQNTYAQLLNGLQH
                     NTTNFLSVVEPPQVPTAPIGPRPIENALLAVLIGFVLSTTGTFLLEYMDDTIKTPEDA
                     RQVFDLAVLGTVGQMDYGDYPSKLVTALQPRSPISESFRILRTNLQFSAVDQPLGALM
                     VTSTSPSEGKSLTAANLAVVMAQSGKRVILVDADLRRPTQHRIFELNNNAGLTNVLLD
                     GPARLADALQLTPIENLRVLTAGSIPPNPSELVGSARMAEFIQSLRAQADLIIFDTPP
                     IMVVSDATVLSPRVDGVLIVVNSAHTRRPIAQRAENTLKAAGARVLGISLNRLAVRDA
                     GYYYDYYYAERDENKPRRWFSRNGHAAKSASVPVPSEEVVKPKPAAAEKA"
     gene            9543..10565
                     /locus_tag="VFF70_13175"
     CDS             9543..10565
                     /locus_tag="VFF70_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZY45691.1"
                     /translation="MSQRIVKVLIFTQASIPDPLIVRLRAVSPRLLIEHHTAQTIEAI
                     DRSVWRDCEVLYTSWLMPTIDLVPNLKWVQGHFAGVDHFLDNPLIRSVILTTASGVHA
                     PHMAEYILMMMLAFAHRLPNMLNYQQRVDWPKDRWSLFAPKELNDSTLGIVGYGSIGR
                     ETARLAKSFGMHVLASKRDVHIKHEQGWQLPKSGDSSAQHVDRLYPPDQLRSMLSECD
                     FVAVTVPLTAETEKMIGAAELRSMKRGAILINVARGGVIDEAALIDALQNNLIGGAGL
                     DVFEEEPLPLNSPLWKLPNVILSPHVSGFSSHYDERAMDLFAENLRRYLTGESLLNVV
                     DMNKGY"
     gene            10648..>11243
                     /locus_tag="VFF70_13180"
     CDS             10648..>11243
                     /locus_tag="VFF70_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45692.1"
                     /translation="MKRIQAHSLTKASITSAREARYRRRMNLRVKTQAEASEFVQDVG
                     FCFLFPIQNVEMPSLWDAIAGRVVRTSASHHGYEIDRTWGWKDDALDKKIWFYGKLIR
                     DKATLVSLDFLPNFYALSENYGDYEHDYLEEYKAGTLTAEAKSIYEALLKHGALDAIR
                     LRRESHLSSDSAKGRFDKALTNLQTGLKVIPVGVAPVGA"
BASE COUNT         2365 a         2928 c         3220 g         2730 t
ORIGIN      
        1 cggtggctgc acgcttgttg gcggtactca tcatgggtaa tcaaattcag accttgcccg
       61 gcatcgatga tcaacgttcg tatgacatgc tggcgcagca agtcgttact ggtcacggct
      121 tcacggtcgt cacagattgg tggccgctga cgcgcgcggg ccaaccgacg gctcattggt
      181 catatctcta cacgctgtat ttggcattca cttacatgat ctttggcgtg cagccgatcg
      241 tggcgcgctt gctgcaagcg atcattgctg gcctcttcat gccgtggctt aattttcgat
      301 tgggccgccg gttagcgaac gatcgtgtcg gattaatggc tgcggcttgg tcagcaatct
      361 atggttactt cgtttactat tcagccgcat tgatgaccga aatgttctac atcacggcga
      421 tcttgtggtc gcttgatctg gccttaagca tggcgaataa tgatcgttcg attaaacgct
      481 gggcggtgtt gggcttatca atgggcatca ccgcgcttct gcgccaaact tttttgctta
      541 ttatcccgat tgttttgatc gcggccttag gtctgcgcgt ttccgacttc aaattgaaaa
      601 cggctcagtc gaaattaacg gctggtctta aaggatcgat cgtggcgctc atggtcatgg
      661 ccgcgctgat tctgccgtgg acgcttcgca actatcaagc cttcaatcgt ttcgttctgc
      721 tcaacaccaa tgcgggcttt gcttttttct ggtctaatca tccgatctat ggtacagagt
      781 tcgtcggtat cttgccgcgt ggcacgtcct atcaaagttt gattccgcct gaattgcgca
      841 cgcttgacga agcgtcactg gatgccgaat taatgaaagc cggtattaat ttcgtcgtgc
      901 aagatccggg tcgctatgcg ttgctgtccc tcagccgctt caaaacttat tttgaattct
      961 ggccgacgag cgaatcggaa ctgttgagta atgtcgtgcg ggtgctgtcg tttggcgccg
     1021 cgctgccatt catgctatat ggtttgattc tcgcattgcg tcactggcga cagtggttgc
     1081 tgttgtatgt attcgcgatc gtgtacagtc ttattcactt gttatcctgg gcgttgattc
     1141 gttatcgctt gcccgtcgat gcagtgctga ttatctttgc ggcctacggc ttggtcgatc
     1201 tcatcgagcg cagtgctcct cgcttaaaac tcaagcctcc taacctagcc tgatggaaat
     1261 tctttttatt gtcccgtaca cgccgacgct catccgggtg cggccctata acttgatcaa
     1321 gtttcttgcg caacgcgggc atcagctcac gctggcaacc ttatgggaaa cggccgacga
     1381 attccgcgcc ttggctgaat ggcgatcaat gggcgtcgat gttctggccg cgcgtttgac
     1441 gcgcgcccga tcgatcctca atgccttaag gactgtgccc acacgcgatc cacttcaagc
     1501 cgcgtattgt gactcaccgc agttaaggca attgatacag tctaaagtta actctcagtc
     1561 ttttgatgcg atccatgttg agcatttgcg cggcgcgcgt tatggcctgc agctccaact
     1621 tctaacttcg aacttcaaac atccaactcc ctttatctgg gacagtgtcg attgcatcac
     1681 ctacctgttt gaacaagcgg cgcggtatcg atccggtttg ttcagctggc tgatcacgcg
     1741 cttggaatta ggccgcacta gaaagtatga agcccgcatg gccgctcagt tcgatcgggt
     1801 gttggtgact tctgaaatag atcgacaagc actggcggcg ttatcgaatc gatcgacgat
     1861 cgatgtgctg cccaacggcg tcgatctgga ttacttcaca ccgcgcctcg atcggcgtga
     1921 agcggacacc gtcgtttttt ccggcaagat gagttatcac gctaatgcgg cggcggcgcg
     1981 ttacttgatc gatctgatca tgccgctggt gtggcggcag cgtccaaccg cgaagttgat
     2041 cattgccggc agccgcccag cgcgatcgat ccggcacgca ggtgctgtcg atgcgcggat
     2101 tgaagttacg ggttcagtag aggatatgcg cgttgcgtta cggcgggcga gtgtttcggt
     2161 tgcgccgctg ttgtatggtg cgggaattca aaataaaatt ttggaagcga tggcgtgcgg
     2221 agttccggtc gtggcctcgt cgcgcgcact ggccgccttg ccacgtgtca aaagaacatc
     2281 tgcggtgtta gtagccgatg agccgcggtc ttttgcggat gcgatcttga gggtgttgaa
     2341 cgatggagag ttgcgcgcac aattgagtgc ggcaggccgg caggtcgttg aggccgagca
     2401 tcattgggat cacatcgccg gtcagttaga agttatttac cgcgcagcaa ttgaacagcg
     2461 cttagcaaaa taattcttcc ctgttcaaat ttgccccgat agctgtacaa tgcaatcgat
     2521 catggatacc tcgctgccgg tgccaagggc gattcccatc acgtacgatt atattaagag
     2581 agcgatggat tttactggct cttggatttt gctgttggtg ctgatcccgt tgctggtgat
     2641 cttggcgctg attgtaaaac tggacagcca tggtccgatc ctgcatcggc ggcgtgtact
     2701 gggtcgtaac ggaacagaat ttgacgcgtt taagtttcga acgatgcagc tcaacggcca
     2761 tgagctgctg gccgcacagc ctgaattgaa agccgcgctg gccgccgagc ataaaatcaa
     2821 aaatgatccg cgcctcacgc gcagcggccg ctggttgcgg aagttgagtc tggatgaatt
     2881 gccgcagtta ttgaatgtgc tgcgcggtca aatgagtttg gtcgggccgc gcatgatcgc
     2941 gccgatcgag ctgtcgaatt acggatcctt cgccgcagag ctgttgacag tgaaaccggg
     3001 cttgaccggc gtgtggcagt tgtcaggccg atcggatttg agctatgccg atcgagtgca
     3061 gctggatatt cgctacatcc gcacccgatc gatctggatc gatctgcggc tgttgttgaa
     3121 aactccgtgg gttgtcatca ccgggcgagg ggcgtactga gatgttgatt gctcgcgcgc
     3181 ccgtgcgggt cagttttttt ggcggtggca cagatctgcc agcgtactat ttgcaatacg
     3241 gcggcagcgt gctgagcacg acaatcaaca agtatgtgta cgtgatcatg aatgtgagcg
     3301 agcaaaacgt gctgcagatc acgtcgtctg attatcagac gttctatcgg catccacccg
     3361 gcgagccgtt attgtgggac ggcgatttga gtttgccgcg cgcggtgctg catcatttcg
     3421 gcgttgagca tggcgtgtcg ttgttcctgg cctcggaagt gccgcccggc accggcctgg
     3481 gatcgtctag tagtgtcatg gtggcattga tcaaagccgt gagtgcggcg cgcggcatga
     3541 agttatcgaa agcacaagtg gccgagatgg ctgtgcagat tgaattggaa aagatgggca
     3601 acccgatcgg cgtgcaggat caatacgccg cggcgttcgg cggcttgaat tgcttcacgt
     3661 tcgattcggc gggcatccat gctgagccga tcgatctgcc ggcgaccacg ctcaagcagt
     3721 tggaatctaa tttgttgttg atattcaccg gctcggcgcg cgattcggcg gagatactct
     3781 cacggcagac acaggccagc cagcagcgtg atccgattgt gattgaagcg ctgcaagcag
     3841 tgcatgaact ttcccggcgc gcgcgcgact atttagtgaa cagtgaagtt gatcaatttg
     3901 gcgctctgct gcatgaagcc tggcagcaca aaaagcgttt tgcggccggt gtgactaacg
     3961 cgcggattga tcaagtctat gagctggcac tgcgtcacgg cgcattaggt ggtaagataa
     4021 cgggcgcggg cggcgggggt ttcttgatgc tctactgtcc cgcggatact atcgaacgtg
     4081 ttgcaggggc gctggcggat gaaggcttgc ggcgcatgga atttcgattt gagcaagacg
     4141 gtgcgcgcgt gttattcaac gcgggcttgc gcttgtgaag ttagaccata gatgcactta
     4201 gggacaatgc ttcggcatgg cagatcacaa acacacgctt aatcgatcga cgcagaccgc
     4261 ctaccaccgt cagtggcaag cgctgattgc tgcgctgatt attttcgacg cgctgatgat
     4321 ctggggcagc ctgcgcctgg cttatgatct gcgcttcacc agcagcctgt tgacttatta
     4381 cggtgagttg agtccggcca cttatgccat catggagatg atcggcctgc ccatctggct
     4441 gggcttattt gcgctggcgg gtttgtatca gcgtaaaaat ttattaggtg gcacgctcga
     4501 atacaagcaa gtgatcaaag ccggcagcgc cggtgtggtc gccatcgtca gtttggcgtt
     4561 cgtttggcgc gatgtggctt tggtgtcgcg cggttggctg gtgttcgcat ggatcttctt
     4621 atgcgcgtcg gtcagcgtgg aacgatttgt aatccgccgg gtggcctatc gattgcgccg
     4681 gcgcggttgg ctgacagccc gggttttgat ggtgggcgca aacgatcaag gcattgcgat
     4741 tgccaagcag tggcagcaat cgcacacttc gggcatgcac gtcgtcggtt ttgtggatga
     4801 tttcaaaccg atcgggacga gcgtgctcga cgatctcaaa gtgatcggac agccgactgc
     4861 gttgctgcag atcatcgatc aatccggtgt ggatgaagtg gtcgttgtac ccaatgccgt
     4921 ggcctgggaa acattcgagg aaatcatcac tcaagccgga gccgttaaag attatgaact
     4981 gtggctgtca cccggcttct atgaaatgtt gaccaacagt gtagtggtga cgaataagtc
     5041 gtatgtcccg ctgtttacga tcaacgcagc gcgcctcgtc ggacctgatg cgatcatgaa
     5101 ggcagttttg gattacggct taggcttcgg cttgttgcta gtcatgttgc cgatcatgag
     5161 cctcattgcc gccggtttga agttgagccg ccgatcggtt ttcatggctc atcggacgat
     5221 cgggcagggc gatacgattt tcttgatgtg gaaatttaac tccgcggacg ataagcccac
     5281 tcgcttgcag cgcggactgc atcgctatgc attagataag ctgcctcaac tgttcaacgt
     5341 gctgaccggc cacatgagct tagtcggccc gcgtccgcgc gtggtcgacg atcagtcgct
     5401 cgatccgcgg caggtgcaca acttgcgatc agtaaagccc ggcctgatcg gcccgtggtt
     5461 ggtgacggaa gtctggtcgt cgttagatga aacgcgcgac gatttgtact acgtgcgcaa
     5521 ttggaccatc tggctggatt tgcaaatcct cttccagtct atcgttgcct ggttccgctt
     5581 gcgccgaccg atgttaatgg ccgaatccga tacgagccgc agcgaagagg cggtgctgac
     5641 agacgacaca tttgaagcgc gccgatcgat cagcggcttg atgcgtttac ccatcaagca
     5701 tggcgcgccg ctgctcatgg acaaagatcg atcgtctcaa aggcttgagt aggatagaag
     5761 tatctcaaat tattaaagac tcggattaaa gcatgttaga ggaatacctc gatcgatatt
     5821 tagacgacat tgcctcggcg ctgaagaagt tggatcgccg cgctgtctgg gccgtcatcg
     5881 aagaactgat gacggcctgg cgcgagcatc gacaggtctt tctgctgggc aacggcggca
     5941 gcgccgcgct ggcttcccac atggctaacg atctgtgcaa gacttcgatc gtgaatggtc
     6001 aaccacgctt gcgggccatc gctttaacgg aaaacgttcc attgctgacg gcgtgggcca
     6061 acgataacgg cttcgagcat attttttccg agcagctcta caacttgtgc cagccgaatg
     6121 atctggtgat cgctatttca tgcagcggca atagtacgaa tgttttgcga gcgctgcgcg
     6181 cggcgcggga actgcgggca cgcacgattg gctttaccgg cgataccggc ggtcaactca
     6241 aagatctggt agacctgtgt gtctttgcgc ccgccgaaaa gatttggccg caagaagatg
     6301 tccacgtgat tttggatcat gtgattgcca gcacgctgca tctgtggatt acggcgatag
     6361 ccgagcagtc ggctcacccg atgcgcggca tgatcttggc ggccggtgca ggcacacgcc
     6421 tgcgaccgct gacgttggat aaacctaaac ccatgctgcc gatcaacggc caaccgctct
     6481 tgcattacac gatcgagtgg ctgcgcgagg cgggcatcca tgaagtggcg atcaatgtgc
     6541 atcatcggcc ggacgcgatc atcgatcact tcggcgacgg atcgcgctgg ggagttaaag
     6601 ttcactactc acatgaagat caattatggg gcacggccgg cgcagtgcgg cggttgaatg
     6661 gttttatcga taatgatgcg ctagtggtgg tttatggcga tgtcctgact aatatggatc
     6721 tgcgcggcct aatggccttc catcgccagc agctggcccg cgatccatcg ctgggtgtga
     6781 cgttgagctt gcagcatctg cccaacccga ttggcaaagg tttggttgag ttggatgata
     6841 tggggcgcgt tacgcgtttt gtggaaaaac cggcggcgca tgacatgttc ggcgacttgg
     6901 tcaacgcggg cgtgatgatc gttgagccga gcgtgatcga tcggattcca cgtgatacct
     6961 tttatgattt cggccagcat ttactgccgg agctgctgca ctcagacaga tcgatctatg
     7021 gttggatcgt tccgcctcag acctacttga tcgatattgg cacgccagag aattacgcgc
     7081 aggcccaacg cgattggccg gtgtccgcga tcgatcgggc ttgatcatgc ggcccgcaat
     7141 ttttctcgat cgagacggcg tcatcaaccg aaatcgcaac gactatatca aagcgtggtc
     7201 ggaattcgag tttctccccg gcagtttgaa tgctttgcag cgcttggcga gtttggaatg
     7261 gccgattgtc gtgatcacta atcaatcggc tgttgggcag gggctgatca gccgcgccca
     7321 agtcgatgag attcaccgcc agatgttaca ggctatcagc gcggccggcg gccggatcaa
     7381 tgccgtgctg tattgtccgc atcgcgctga tgaaggctgt acctgccgca aacctcatcc
     7441 gggtttaatc ctgcaagttg ccgagcattt agcgatcgat cttccgcaca gttttttcat
     7501 tggcgatgcc gaaaacgata tcttggcggc gcgggctgcg ggttgccagc cgatcttagt
     7561 caagaccggg cgcggtgttc aggagtggac tatcctgcgg caacatcaag tgtccggctt
     7621 ccaggtcgct gaagatttgg gcgaggtcgc agactggctc tgccagctag cggctactga
     7681 ctttggcgca gcagaatgaa ctaagtgtag taaaataggc ggcgtcttgg tcgggctgaa
     7741 cggaagttcg atgaaacgcg gcttctatat catctaaagg attgttacat ggaattgcgt
     7801 cagtatttga atattgtccg caagtggtgg tggctgattg tgatcagcat ggtcattgcc
     7861 ggcggcgccg gttattttgc tacaaagtct attccaccca cctacttatc gcgtgtgacg
     7921 ttgatggtgg ggcaagcctt acaaaaccct aatcccagcc aaaacgagat ttttacgggc
     7981 gaagcattag cccagagcta tgccgatttg gttaagcgtc agcccgtgtt gcaaggcacg
     8041 ttaaatgcct taagtctgaa ttgggagctg accgcactcc agcaaatggt cagcagtcga
     8101 gtggtgctgg gcacgcagct cttggagatt acagttttgg acactgatcc aaatcgggca
     8161 cagacgttgg ccaatgaaat aggacatcaa gcgatcttgc tgagcccgtc gagtaccgat
     8221 ctcggcgaag ctgatcagcg ccaattcatt ctcgatcaac tggtcgatct caaagcgaat
     8281 attcaacacg cgctaactga aaacgctcaa ttagatcaga cgatcaccag caccggcagc
     8341 gcttcgcaag tgcaagcggc gcgcgatcga caggccgtgc tgcggactca aatttcttca
     8401 tggcaaaata cgtacgcgca attgctcaac ggcttacagc acaataccac gaactttttg
     8461 agcgtcgtcg agccgccaca agtgccgact gcgccgattg gtccacggcc gattgagaac
     8521 gcattactgg ccgtcctcat tggctttgtt ttgtcgacta ccggcacttt tcttttggaa
     8581 tatatggacg acacgatcaa aaccccggaa gatgcccggc aggtttttga tcttgcggtg
     8641 ctaggcaccg tcgggcaaat ggactatggc gattatccgt ccaagctggt gactgctctc
     8701 cagccgcgtt cgcccatctc cgaatctttt cgcattctgc gcactaacct gcaattcagc
     8761 gccgtcgatc aaccgctggg cgcattgatg gtcaccagta ccagcccgtc tgaaggcaaa
     8821 agcctgactg cggccaatct ggcggttgtg atggcgcaat cgggcaaacg tgtcatccta
     8881 gtcgatgccg atctgcgccg cccgacgcag catcgtatct ttgaactcaa taacaatgcc
     8941 ggtttgacca atgtcttgct ggacggccca gcccgcttag cggacgcgtt acaactgacc
     9001 ccgattgaaa acttgcgagt gctgactgcc ggttccattc cgccgaatcc gtctgagtta
     9061 gtcggttcgg cacgcatggc cgagttcatt caaagtttgc gtgctcaagc cgatctgatc
     9121 atctttgaca cgccgccgat tatggtcgtc agtgatgcga ccgtactgtc accgcgcgtt
     9181 gacggggtct tgatcgtggt caattctgca cacacgcgtc gaccgattgc gcagcgcgcc
     9241 gaaaacacgc tcaaagcagc gggcgcacgc gtccttggaa tttctctcaa tcggttggct
     9301 gtgcgtgatg ccggttatta ctacgattac tattatgccg agcgcgatga gaataagccg
     9361 cgccgctggt ttagccgcaa cggccatgcc gccaaatccg ccagtgtgcc tgtgccttca
     9421 gaagaagtgg ttaagcccaa accggccgcc gccgaaaaag cctaacgctt cacggttgcc
     9481 ttgacgctca acgactccac gcccgtggag tcgtttttgc tctactgatt caggagcgca
     9541 gcatgtccca gcgaatcgtc aaagttctca ttttcactca agcatccatc ccagatccgt
     9601 taatcgttcg actgcgtgcg gtgtcgccgc gcctcttgat cgaacatcac accgcgcaaa
     9661 cgatcgaggc gatcgatcga tcggtgtggc gggattgcga agtgttgtac accagctggc
     9721 tgatgccaac gatcgatctc gtgccgaatc tcaagtgggt gcagggacat tttgccggcg
     9781 tcgatcattt tctggataat cccttaatcc gatcggtgat cctgacgacc gcgagtggag
     9841 tccacgcgcc gcacatggcc gaatacattc tgatgatgat gctggccttt gcccatcgct
     9901 tgcccaacat gctcaactat caacagcgtg tggactggcc gaaagatcgc tggagcctct
     9961 tcgcgcccaa ggaactcaac gactccacac tgggcattgt cggctacggc agcatcggcc
    10021 gcgagacagc gcgcctggcc aaatcttttg ggatgcacgt gctggcaagc aaacgcgatg
    10081 tgcacatcaa acatgagcag ggttggcagt tgccgaaaag cggcgactcg tccgcgcagc
    10141 atgtcgatcg attgtatccg cccgatcaac tgcgctcgat gctgagtgag tgcgacttcg
    10201 tggccgtgac agtgccgttg accgctgaga cggaaaaaat gatcggggcc gccgaactcc
    10261 gatcgatgaa acgtggcgcg atcttgatca acgtcgcgcg cggcggtgta atcgacgaag
    10321 cagccttgat cgatgcgctg cagaacaacc tcatcggtgg cgcggggctg gatgtgtttg
    10381 aagaagaacc tttacctctg aatagtccct tatggaagtt gcccaacgtc attctcagcc
    10441 cgcacgtctc cggctttagt tcccactatg acgaacgcgc gatggatctg tttgccgaaa
    10501 acttgcggcg ttatttgact ggcgaatcgc tgcttaatgt ggttgatatg aacaaggggt
    10561 attgattcaa aagatgtgga agcatcctga gcggaacagc gaactcgaag agagcgcagt
    10621 gaagccgaag gacgcgatta acaaaacatg aaaagaattc aagcacattc actcacaaag
    10681 gcctccatca cttctgctcg tgaagcccgc tatcgacgcc ggatgaatct gcgcgtgaag
    10741 acccaagcgg aagccagtga gttcgtccag gatgttggtt tctgctttct ctttccaatt
    10801 caaaacgtcg aaatgccatc gctgtgggac gcgatcgccg gtcgggtggt gcgaacctct
    10861 gccagtcatc acggttacga gatcgaccgc acgtggggtt ggaaggatga tgccctcgat
    10921 aagaaaattt ggttttacgg caaactcatc cgcgataaag ccacgttggt tagtttagat
    10981 ttcctcccga acttttatgc gctgagcgaa aactatggcg attacgaaca tgattatctg
    11041 gaagaataca aagcgggcac actcaccgct gaagccaaat cgatctacga agcgctgctc
    11101 aaacatggcg cgctcgatgc gatccgcttg cggcgtgaat cgcatctcag ttccgattca
    11161 gccaaaggcc gcttcgataa agcgctgact aatttacaaa ccggcttgaa agtcattccg
    11221 gtcggcgttg cgcccgtcgg cgc
//
LOCUS       DASRTJ010000649         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_364459,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000649 DASRTJ010000000
VERSION     DASRTJ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364459"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..580
                     /gene="moaC"
                     /locus_tag="VFF70_13185"
     CDS             101..580
                     /gene="moaC"
                     /locus_tag="VFF70_13185"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZY45693.1"
                     /translation="MSKSLTHLDEHGHARMVDVGGKADTKRVAIARGEVHLSHATIDL
                     ILSHTAPKGDVFAAARLAGIMAAKKTSELIPLCHPLSLTHLSVDLKGHLATGIVEITA
                     QAETTGKTGVEMEALTAVSVAALTVYDMIKAVQRDARIMNVRLIEKHGGKSGDIVLE"
     gene            626..1249
                     /locus_tag="VFF70_13190"
     CDS             626..1249
                     /locus_tag="VFF70_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZY45694.1"
                     /translation="MTNIEARSIVVKQAQQTEIFTISYNALEAIAAIGLGMASRSTSL
                     ATFGLDSLIEIGAGLILVWRLNAEQRSADTARIEFVERRAAKLVGWSLLGLAVYVAIQ
                     SVSELITQAKPDPSIWGIVLAVASLILMPFLGRRKLQLADKISSKALHADAYETIACA
                     YLSFTLLLGLTANYLFGWWWADAIAALAMLYFIVREAREALSGEHAE"
     gene            1256..1966
                     /locus_tag="VFF70_13195"
     CDS             1256..1966
                     /locus_tag="VFF70_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZY45695.1"
                     /translation="MNVRIKLFATLKDRAGSNHVEIDLPDDATVAMLLERLALAQPTL
                     APSLPTSLVAVNQEFAFGSTLIRSGDEIALFPPVSGGSIVYPEIFRIVDQQIDLNELV
                     TSVTLPSTGAVCTFTGTVRGVSSDHETLQLNYEAYTPMAEAKMKQVAAEIRSRWPDVQ
                     GMAMVQRVGQLTVGEFTVLIAVSAGHRDQGVFEAARYGIDRLKEIVPVWKQEIGPQVE
                     RWVEGHYHPTEKDVKSNT"
     gene            2022..3272
                     /gene="fabF"
                     /locus_tag="VFF70_13200"
     CDS             2022..3272
                     /gene="fabF"
                     /locus_tag="VFF70_13200"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZY45696.1"
                     /translation="MTGKKRVVVTGMGTLNPCGSTVEETWQNIIAGNSGIDTITLFDT
                     SRLNVHIGGQVKKFDPAKYMDTKEARRRDRFCQLAVAATKLALEDSGLVITEENADDI
                     GCIYGTAVGGLKSFFDASVVDVTDGPRKLHPFSIPMTIADGASGSIAIDWNIRGPNFA
                     PVSACSAGADAIGLSYQAILLGQAKAMVCGGSETPLIEIGIGAFDRLGAMSHNNDDPK
                     HACRPFDANRDGLVMGEGAATLILEDLDYALARGANIYAEIVGYGCTSDAFHVTAPAE
                     NGVGAARAMKRSLENAELQVTDIDWISAHATATALNDKYESIAIKSVFGARAYKIPIS
                     GTKSMTGHMMGATGALEVIFGIKAIETSTLPPTINFESGEMPDCDLDYVPNKARPQKV
                     NTFMSNAFGFGGHNSVLIVKRFVA"
     gene            3341..>3891
                     /gene="rnc"
                     /locus_tag="VFF70_13205"
     CDS             3341..>3891
                     /gene="rnc"
                     /locus_tag="VFF70_13205"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZY45697.1"
                     /translation="MLPPFNNSALLTRALTHRSYLNEHPELPGEDNERLEFLGDAVLD
                     FVAAEYLFRQYPAFAEGRLTSIRAALVRMETLSEYANQLDIGSLLRLGKGEAETGGRA
                     RQSLLADAFEAVVGALYLDQNIDAVRNFIEPLLAAEADRIVGDERDRDAKSILQEWCQ
                     ANRNTTPHYRTISAVGPDHAKWFT"
BASE COUNT          965 a          987 c         1078 g          861 t
ORIGIN      
        1 tgtggcgcga actaagagaa gagggacgaa cgacaaaaga ccaccgaccg acaaaaaatt
       61 taaactaaaa tttgtcgttg gtcatcggtt gatgatcact atgagcaaat cactcactca
      121 cctcgatgaa cacggccacg cccgcatggt cgatgtcggt ggcaaagccg acacgaagcg
      181 cgttgccatc gcgcgcggcg aagtgcatct ctcacacgca acgatcgatc taatcctgag
      241 ccacactgcc cccaaaggtg atgtgtttgc ggcggcacgc ctcgccggaa ttatggccgc
      301 caagaaaacg agcgaactca ttccattgtg ccatccgctg tcgctgaccc atctttcggt
      361 tgatctcaag ggacacctcg ctacgggcat cgtcgagatc acagcccagg ccgagacaac
      421 cggcaagacc ggcgttgaga tggaagcgtt gaccgctgtc tcagtagcgg cgcttaccgt
      481 gtatgatatg atcaaagccg tgcagcgcga tgcacgcatc atgaacgtcc gcttgatcga
      541 gaaacatggc ggcaaaagcg gcgacatcgt tttggaatga tcgcagttga tcaacctagc
      601 ctcatcaatt tctcaagatt aaccaatgac gaacatcgaa gcacgcagca tcgtcgtcaa
      661 gcaggcacag cagaccgaaa tctttacgat ctcatataac gcgctggaag ccatcgccgc
      721 aataggactg ggcatggcaa gccgcagcac ttcgctcgca acatttggac tggacagcct
      781 gatcgaaatt ggggcgggcc tcattctggt gtggcgcttg aatgccgaac aacgcagcgc
      841 cgatacagcg cggatcgagt tcgtcgagcg gcgagcggcc aagttagtag gttggtcact
      901 cttgggactg gccgtctatg tcgcaataca atcagtcagt gagctaatca cgcaagccaa
      961 acccgatcca agtatttggg gaattgtact ggctgtcgcc tcactgattt tgatgccgtt
     1021 tctgggtcgc cgcaaattgc aactggccga taagatcagt agcaaagcct tgcatgccga
     1081 tgcatatgaa acaatcgcct gtgcttattt gtcattcact ttgcttttag ggttaaccgc
     1141 aaattattta ttcggttggt ggtgggctga tgcaatcgcg gcgctagcga tgttgtactt
     1201 cattgtgcgt gaagcgcgcg aagccttatc gggcgagcat gctgagtgaa gtgagatgaa
     1261 cgttcgaatt aaactctttg ccaccttaaa agatcgggcg ggcagcaatc acgttgagat
     1321 cgatttaccg gatgatgcaa ccgtcgccat gctgctcgag cgattagcgc tggctcaacc
     1381 tacactcgcg ccgtcattgc caacctcatt agttgcagtg aatcaagaat ttgcttttgg
     1441 gtcaacgctg atccgatcgg gtgatgagat tgccttattc ccgccagtca gcggcggttc
     1501 gatcgtgtat ccggaaattt tccgcatcgt cgatcagcag atcgatctga atgaacttgt
     1561 cacctcggtc acgctgccgt cgaccggcgc cgtgtgcacc tttactggaa cggtgcgcgg
     1621 cgtcagcagc gatcacgaaa cgcttcaatt gaattacgag gcttacacgc cgatggccga
     1681 agcgaagatg aaacaagtcg ccgctgaaat tcgatcaaga tggccggatg tgcagggcat
     1741 ggcgatggtg cagcgcgtgg gacaattgac tgttggcgag tttacggtcc tgattgcagt
     1801 ttcagcaggt catcgcgatc aaggtgtgtt tgaagcggca cgttacggca tcgatcgact
     1861 gaaggagatc gtgccagtgt ggaagcaaga gatcggtccg caggttgaac gctgggtgga
     1921 aggacattat cacccaacgg aaaaagatgt gaagagtaat acgtgattga tttttacgca
     1981 gcacgcatta tcaatagagg atcgttttaa tggagacaag tatgactggc aaaaagcgcg
     2041 tggtggttac cggcatgggc acactcaatc catgcggatc aaccgtcgaa gaaacctggc
     2101 agaatattat tgccggaaat tccggtattg atacgatcac gctgtttgat accagccggt
     2161 tgaatgtaca catcggcggt caggtgaaaa aattcgaccc ggcaaaatac atggatacca
     2221 aagaagcgcg gaggcgagat cggttttgcc aattggccgt ggcggcgacg aaactcgcgc
     2281 tggaagattc gggcttggtc attactgaag agaatgcaga cgacattggc tgcatctacg
     2341 gtacggcagt gggcgggctg aaaagtttct ttgatgcttc ggtcgtcgat gtgacggatg
     2401 gcccgcgcaa gctgcatccg ttttcgatac cgatgacgat tgccgacggc gcatccggct
     2461 cgatcgcgat cgattggaat atacgcggcc caaacttcgc gccggtctcg gcttgttcag
     2521 cgggcgctga tgcgatcggc ttaagctatc aagcgattct cttgggccaa gccaaagcga
     2581 tggtctgcgg cggcagcgaa acaccgttga tcgaaatcgg cataggcgcg ttcgatcgat
     2641 tgggcgcgat gagccacaac aacgacgatc caaaacatgc ctgccgtccg ttcgatgcga
     2701 atcgcgacgg gctggtcatg ggcgaaggcg cggccacttt gattttggaa gacctcgatt
     2761 acgcgctggc acgcggcgca aatatttatg ccgaaatagt tggctatggc tgcacatccg
     2821 atgcgttcca tgtgactgca ccagctgaaa atggtgtcgg cgcggcgcgc gccatgaaac
     2881 gctcactgga aaacgccgag ctgcaagtca ccgacatcga ttggatcagc gcgcacgcga
     2941 cagcgacagc cttaaacgac aagtacgaat cgatcgcgat taaaagtgta ttcggcgcac
     3001 gagcttataa gattccgatc agcggcacga agtcaatgac cggtcacatg atgggcgcaa
     3061 caggcgcatt ggaagttatt tttggcatca aagcgatcga gacttccacc ctgccaccga
     3121 cgatcaattt tgaatcaggc gaaatgccgg attgcgatct ggactatgtg ccgaataaag
     3181 cccgcccaca aaaagtcaat acattcatga gcaacgcgtt cggttttggc ggacacaata
     3241 gtgtgctgat tgtaaaacga tttgtggcct aacgagtaat gagtaacgag taattcacaa
     3301 ctagcaatca ctcattcctc gtcactcatt atttttcaac atgctcccac ctttcaacaa
     3361 ctctgctctc ctgactcgtg ccctcacaca tcgatcgtat ctgaacgaac atcccgaact
     3421 gcccggtgaa gacaacgaac gcttggaatt tctcggcgat gccgtcttgg acttcgttgc
     3481 cgctgaatat ttatttcggc agtatcccgc atttgctgaa ggccgcttga catcgatccg
     3541 cgccgcgttg gtgcgcatgg aaacgttgag cgagtatgcc aatcaattgg atataggcag
     3601 tttattgcgc ttaggcaaag gtgaagccga aacaggcgga cgcgcgcggc agtcgctgct
     3661 ggccgatgcg tttgaagcgg tcgtcggtgc gctgtatctc gatcaaaata ttgacgcggt
     3721 taggaatttt attgagccgc tgcttgccgc agaagccgat cgaattgtgg gcgacgagcg
     3781 tgatcgcgac gcgaaaagta ttttgcaaga atggtgtcag gccaatcgaa acaccacgcc
     3841 gcactatcga acgatcagcg cggtgggacc ggatcacgcc aagtggttca c
//
LOCUS       DASRTJ010000650         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_136767,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000650 DASRTJ010000000
VERSION     DASRTJ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136767"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFF70_13210"
     CDS             complement(<1..1453)
                     /locus_tag="VFF70_13210"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HZY45698.1"
                     /translation="MNDPLPETNVFEVHLPKPSPRGLIVKIAAIVLGVGVIAVAAGVI
                     INRAASPTIDPLAQIMPADTMLFLSMTTHPDQQPNYAVVADAWKGSKEAKQVESGLAV
                     AITYAGFDWDTDIKPWLGDRAGVGLIDLGGTDQPPFFIIAIQTRDHAKSDKFLADYRQ
                     QRQTSAQGTFAIHDETYRGISIVYVSNFSQYAPYAEAFATVNDVVVVTLGPDDLKKAI
                     DAALDGKTLATSSNFKTTMMALPGQNVGAMYMDLANYLTAASKMFDATTLETPSALLQ
                     RQQSMQQTQDMLQALGGVGLAMTYEPTGLRFDMVEQIDPSKLPEKWRSLYPTSYTAAS
                     NQIFDNIPASAFFAMNAHLPASTVQLFLDPNYWSTVFAANPNVNSADIVQKIDEFQKA
                     AGVDLKADLLDLHNGDMAVVMMTKSNLPAPNPNAFNSFNFPFDLAALVDSSDALRTAS
                     SLDKIFGALIASSDQSGVQLQPMNRAPYTALLDQ"
     gene            complement(1595..>1839)
                     /locus_tag="VFF70_13215"
     CDS             complement(1595..>1839)
                     /locus_tag="VFF70_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45699.1"
                     /translation="VGMSLGGSIALTWGTQRVQTLVAQFAAGSNQFAGNTLDFFRRAT
                     TQAVNESFVLFAALACVIALLPALLMRKENLDAVYD"
BASE COUNT          418 a          480 c          516 g          425 t
ORIGIN      
        1 gctgatcgag cagggccgta taaggcgcgc gattcatcgg ctgcagctgc acgccgctct
       61 gatcggatga agcgatcaat gcgccgaaga ttttatcgag actgctcgcg gtgcgcagcg
      121 catcagacga atcgaccagc gcggcgagat caaatgggaa gttgaaagaa ttaaaagcgt
      181 ttggattcgg tgcgggcaga ttggacttcg tcatcatcac tacagccata tcgccgttat
      241 gcagatcaag cagatcggct ttgagatcta cccccgctgc tttttggaat tcatcgatct
      301 tctgcacaat gtcggcgctg ttcacattcg gattcgccgc gaagaccgtc gaccagtaat
      361 tgggatccag gaacagctgc acagtgctgg ccgggaggtg ggcattcatg gcgaagaaag
      421 ccgaggccgg aatgttatcg aagatttgat tagaggcggc tgtatagctg gtcggataca
      481 agctgcgcca cttctccggc aatttgctcg gatcgatctg ctccaccatg tcaaagcgca
      541 ggccggtcgg ttcgtaggtc atcgctaacc cgacaccgcc cagcgcctgc agcatgtcct
      601 gcgtctgctg catcgattgt tggcgttgca acagagcgga tggtgtttcc agcgttgtag
      661 cgtcgaacat ctttgatgcc gcggtcagat aattcgctaa atccatatac attgcgccga
      721 cgttctgccc gggcagcgcc atcatcgtag tcttgaagtt gctgcttgtg gctagggttt
      781 tgccatccaa cgccgcgtcg atcgctttct tcaaatcgtc cgggccaagg gttaccacga
      841 ccacgtcgtt gaccgtcgcg aaggcttcag catatggcgc gtattgagaa aagttgctga
      901 catagacaat tgatatgcca cgatatgttt cgtcgtgaat agcgaaagta ccttgcgcgc
      961 ttgtttgacg ctgctggcga taatctgcta agaacttgtc cgacttggcg tgatcgcggg
     1021 tctgtatcgc gatgatgaag aacggcggct gatcggtgcc accgagatcg atcagaccta
     1081 cacctgcccg atcgcccagc cacggtttga tgtccgtgtc ccaatcgaac ccggcatagg
     1141 tgatagcgac cgctaaaccg ctttcaacct gcttcgcctc tttcgatcct ttccacgcgt
     1201 cagccacgac agcgtagttg ggctgttgat caggatgcgt ggtcatcgat agaaacagca
     1261 tggtgtcggc gggcatgatc tgcgccagtg gatcgatcgt gggcgaggcg gcacgattga
     1321 tgatcacgcc agcggcaacc gcgatgacgc ccacgcctaa tacgatcgcg gcgatcttga
     1381 cgatcaggcc gcgcgggctg ggcttgggca gatgaacttc aaatacattg gtttcgggca
     1441 gtgggtcgtt catgagatac tccttgttga attgcacagc gaaaggttga cgtattatca
     1501 gatcgatctt caaaaataat caagcactta agtgcaggct tatcggcggc tgaaagtcac
     1561 atgcgatcat ataagaattg aacaatctgc gtcttcagtc ataaactgcg tctaaatttt
     1621 cttttcgcat taataacgcg ggcagcagcg caatgacgca cgcgagtgcc gcaaataata
     1681 cgaaagactc attaacggcc tgagttgtgg cgcggcggaa aaaatccaga gtgttgcctg
     1741 cgaattgatt cgatccggcg gcgaactgcg cgactaaagt ctgtacgcgc tgagtgcccc
     1801 acgtcaacgc gatcgatccg ccgaggctca tgccgacca
//
LOCUS       DASRTJ010000651         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_290234,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000651 DASRTJ010000000
VERSION     DASRTJ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290234"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VFF70_13220"
     CDS             complement(<1..1643)
                     /locus_tag="VFF70_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZY45700.1"
                     /translation="MSTTTRQRFSNPRIVVLLAVSLAIMALFVLRLWDLQIIRGADFR
                     ALADQNRFRLESVDAPRGIIYDRNGIPLVRNAPSFEIQIIPAYLPDDPAAEHAVFQYL
                     SGMLNLPIESQPIQTSEVVPLDRGAVSLASTLHFMIDRLKEKIEVRGCCIKDIVEGVR
                     NLAPYSPATIKSGVNRDIALRIAEQANNLPGVRVHVASVREYISGTVLSDIVGYMRRI
                     SAEDLATLPADTYDPSSDRIGAVGVEGLFEDVLRGQKGRQYVEEDVVGRQVRIVGQTD
                     PIVSGQNVQLTIDFDLQQYAKQALQDEIDFLNRYAGHTVTQQGVVVAMNPKTGEVLAM
                     VSLPTYDNNIFSSSFISQSVLDQLQTDPYLPQLNHAIQSVYPPGSTFKIVPASAALQE
                     GVLTPKTIIYDPGAIVIPNQYFPDDRRQAQTFVCWLRSGHGDENILQALAHSCDVFFY
                     EVAGGYHVKGQTEFEGLNVDRLGKYAQLFGYGTPTGIDLVGEAAGFVPDAVWKRRTTG
                     ENWSLGDTYNFGIGQGYLTATPLQVLNSMAAIANDGILM"
BASE COUNT          461 a          521 c          495 g          410 t
ORIGIN      
        1 ttcatcaaga tgccatcgtt tgcaatcgcc gccatcgaat tcaaaacttg cagcggtgtc
       61 gccgtgagat aaccttgtcc aatgccgaag ttataggtat cgcccaacga ccagttctcg
      121 cccgtggtgc gccgcttcca gactgcatcg gggacgaagc cggctgcttc accgacaaga
      181 tcaatcccgg tcggcgtgcc gtagccaaag agctgcgcat acttccccaa ccgatcgaca
      241 ttcaagcctt caaattctgt ctggcctttg acgtgataac cgcccgcgac ttcataaaag
      301 aacacgtcgc aggaatgcgc caaggcttgc aaaatatttt cgtcgccatg cccgctgcgc
      361 agccagcaaa caaatgtttg cgcttgacgt cgatcgtccg ggaaatattg attgggaata
      421 acgatcgcac cgggatcgta aatgatcgtc ttgggcgtca ggactccttc ttgcaaagcc
      481 gctgaggccg gcacaatttt gaaagtcgag ccgggtggat acacactttg aatcgcatga
      541 ttcaattgcg gcagatacgg atcagtctgg agttgatcaa gcacgctttg gctgataaag
      601 gaactggaga agatattatt gtcataggtg ggcagactaa ccatcgccaa gacttcgccg
      661 gtctttggat tcatggccac gaccacgccc tgctgggtga ccgtatgacc cgcatatctg
      721 ttgagaaaat cgatctcgtc ctgcagagct tgcttagcgt attgctggag atcgaaatcg
      781 atcgtcagct gaacgttttg cccggacacg atcggatccg tttgcccgac gatccgcact
      841 tggcgcccca ccacatcttc ttcaacgtac tgcctgcctt tttgtccgcg caagacatct
      901 tcaaatagtc cttcaacacc caccgcaccg atgcgatctg atgatggatc atacgtatcg
      961 gcaggcaatg tggctaaatc ttcggccgag atgcgacgca tgtaaccaac aatatcggac
     1021 agcactgtac cggagatata ttcgcggacg gacgccacat gcacgcgcac accgggcaga
     1081 ttgttggctt gctcggcaat gcgcagcgct atatctcgat tgacgccgga cttaatggtg
     1141 gcaggcgaat acggcgcaag gttgcgcact ccttcgacaa tgtcctttat gcagcaaccg
     1201 cgcacttcaa ttttttcctt caatcgatcg atcatgaaat gcagcgtgct tgctaaactg
     1261 actgcgccgc gatccagcgg caccacttcc gaggtttgga tcggctgcga ttcgatcggc
     1321 aggttgagca tccccgacaa atactgaaag acggcgtgct cggcggcggg atcgtctggc
     1381 agataggcag gaatgatttg gatttcaaaa ctgggcgcgt tgcgcaccag cgggatgcca
     1441 ttgcgatcgt agataatgcc gcgcggcgca tcgacagatt ccaagcggaa gcggttttga
     1501 tcggccaaag cgcgaaagtc tgcgccccga atgatctgca agtcccacag gcgcagcacg
     1561 aacagcgcca tgatcgccag tgaaacggcg agcagcacta caatgcgcgg attagaaaaa
     1621 cgttgtcgag tggttgtact cataaaatgt ttaaccgcca acgccagcgg cataccgctt
     1681 cgcggtcacg ggcaaaccca cggcatgccg agactgctaa aatatcttag ggtcgctgca
     1741 gataaaactc gctaccacgt taagcccggt ggagtgaccc gtcgatgcag tgccgacatc
     1801 aatcgataag cgatcgggct gaggatcata ttgagcaccg cggaaggcag cataacccgt
     1861 atgatcgcgt caaaaatatc gatcttc
//
LOCUS       DASRTJ010000652         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_364825,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000652 DASRTJ010000000
VERSION     DASRTJ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364825"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(662..>1687)
                     /locus_tag="VFF70_13225"
     CDS             complement(662..>1687)
                     /locus_tag="VFF70_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45701.1"
                     /translation="TLMTKTAIAFAHTGLMFLMARTAEGPIVKEIKPGAMVGYADIVR
                     RVIKAKDGKVMSVFASQTEKLGSTLMLRELEEKFQRLGDLEMAIVDTGENGLFAKGEF
                     EAITHIWQMEFVTPEEIAQQVVMEIKGGNTGYDMIGAIDAAVMNPTYRAGYLRSFVLQ
                     EVSRLEDMDQPSVAIGRLGPPELGKLLWEAQLLKTKYGSISSVLRKSADDLARELAEL
                     IRSEAGTNLRQRIISIGLPILTPDGSTLIRGPFLRIPEVPNMTSVPVTPDRIDQWAMK
                     GWVDLRAKNFAVWLDRFARMKRENEQTHGRGSAAITLQTYWSDEISIGAVAGWIFNNE
                     EGGFRIK"
BASE COUNT          365 a          513 c          402 g          407 t
ORIGIN      
        1 ctcttctatg gacaacttcc ccaagtcgcc aaggatcgtc atcaacggcg attttttgcc
       61 aagatattcg acgcgccaga cttcgagggc atccttgctc tcgatcgagc ggagcgcgtt
      121 gagcgcggct tgttcaatgg atgtaagttg attgagcata tgagcctcca aaaacattta
      181 tacaggagag cgcgccaaga tcgcaaagta aatgagaatc tcagcgccct ccggtaccca
      241 ctgcgtgctt taccgcaagt gtgcgatctc agcagcgaga tcgatgtacg cgattaaaat
      301 aaaaacgccc tcatcctcac agggacgaga gcgttagcgc tgccgtggta ccaccccact
      361 tgatcgatct tcgatcgatc cactttgtcg ccgacgatca tcggctgtcc cgttaacgca
      421 ggacatgcgg aggaaactac tctcgacgcc tgtgcatcga tttcacttct cggctcggga
      481 gcgaacttcg gtggcggtta ctcgatcgag gcttgcagtc attgaccccg attccctgtc
      541 gaatagctgg ccgcttactt ttctccgtcg ttgcctaatg cctgtattat ccgcatctcg
      601 attaatttgt caagcacttg acgaacgacc aacgaccaac ttattggtca tttgtaattt
      661 gtcatttaat tctgaagccg ccctcttcat tgttgaatat ccagccagcc accgcgccga
      721 tagagatttc atccgaccag taagtctgca gcgtgatcgc cgccgagcct ctgccatgtg
      781 tttgttcgtt ctcgcgcttc atccgcgcga atcgatccag ccacactgca aagttcttgg
      841 cacgcagatc gacccagccc ttcatcgccc actgatcgat gcggtccggc gtcacgggca
      901 cgctggtcat attcggcact tcgggaatgc gcaggaacgg cccgcggatg agcgttgatc
      961 catcgggcgt gaggatcggt aaaccgatcg agatgatgcg ctgccgcaag ttggtcccgg
     1021 cctccgatcg gatcagctca gccagctcgc gcgctaaatc atccgccgat ttcctcaaca
     1081 cgctgctgat cgatccatac ttcgtcttga gcagctgcgc ttcccataaa agtttgccga
     1141 gctctggcgg gccaagccgg ccgatcgcca ccgacggctg atccatatct tccaatcgag
     1201 aaacttcttg caggacgaag ctgcgcaaat aaccagcgcg gtaagtggga ttcatcaccg
     1261 ccgcgtcgat cgcgccgatc atgtcgtagc cggtattgcc gcctttgatc tccatcacga
     1321 cttgctgcgc gatttcttcc ggtgtgacaa attccatttg ccagatatgc gtgatcgcct
     1381 caaattcacc tttggcaaac aacccattct ctcccgtatc gacgatcgcc atttccaaat
     1441 cgcccaagcg ctgaaatttt tcttccaact ccctcagcat caacgtcgat cctaactttt
     1501 ccgtctggct ggcaaacacc gacataactt tgccatcttt ggctttaatc acgcggcgca
     1561 caatatccgc ataaccgacc atggcgccgg gcttgatctc tttcacgatc ggaccttcgg
     1621 cggtgcgcgc catcaagaac atcaagcccg tatgcgcgaa ggcgatcgcc gtcttggtca
     1681 tcaaggt
//
LOCUS       DASRTJ010000653         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_137023,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000653 DASRTJ010000000
VERSION     DASRTJ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137023"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1923
                     /locus_tag="VFF70_13230"
     CDS             409..1923
                     /locus_tag="VFF70_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZY45702.1"
                     /translation="MLIVLIVAVVLLLSGILGLGNLPAADPIVGISTRTATATPTSTV
                     TPTPTFIPTSTSTATPTNTPTPTSPPRPSGAILPLIFVTAGEVISGTPVPTQMPYIDQ
                     PEGSINILLLGTDQRPTSGGYRTDSIFVIGVNPQRQYVTMLSIPRDLWVYVPIEDKFD
                     RINTVDAWGESHKVPGGSASLIKQTLLYNLGIPIDYYARIDFSGVKQIVDRVGGVEVL
                     AFCPLYDVFPDVSDDQSDIISDPALLATVLTGTIDIPSPGLYTLDGKHALWFARSRKS
                     TGDFDRSRRQQQVLRAIWAKIKANGLISQLPSLWGDVTSIVQTDLSVNDVGYLATFAA
                     QLDDSRIKQRAIDKLSTDGFVAANGAEVLAIAPDRVSAVLQEAFEPPLTNVTSQASAQ
                     IEVWNGSGQANWDLLAINRLRSEGFVVTSYGPADHVYSQTQVINFNTTVKGSRSSQLA
                     RLFNVRSSSNLIDQPTANSPINYRLIVGSDFNSCLPPPAPVQFPTPTPTASPVP"
     gene            complement(2108..2869)
                     /locus_tag="VFF70_13235"
     CDS             complement(2108..2869)
                     /locus_tag="VFF70_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZY45703.1"
                     /translation="MKTTYAVRHLAILAGVSVRTLHHYDQIGLLKPTSRTAVGYRQYG
                     EKDLLRLQQILFFKELDVPLSEIQAILDRPGFDQVKALKDHKGLLTQRADRLTRLLKT
                     IDATIDKLSEAKMSLTDADLYEGFSQEQIDRYKREVRELYDVKIVEESEQRVGKMSKQ
                     QWAAIKAEGDAITRELAGLMDKPIANRLVQQAIGRHYAWVGNFYTVTPEIFRGLGQGY
                     AEHPEFRATYDKYRTDMADFMQAAMTYYCDHTLAK"
     gene            complement(2945..>3478)
                     /locus_tag="VFF70_13240"
     CDS             complement(2945..>3478)
                     /locus_tag="VFF70_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY45704.1"
                     /translation="NDIHFRSKRMTHPLIDQFHFTRSEWLRGIEGVSEEDGARHFGQM
                     NSIGWTVGHLAWHEQRSWLTRPQGKILFPQLITDFAFGAPMSTPSFNAMLDIWHMVTK
                     AADPFLDSLTTELLLTELLVDGKAVGQSIGSALRRVTYHYWYHIGEIQAIRQILGQSN
                     LPEYVGDIETEAPYRAE"
BASE COUNT          765 a          986 c          903 g          824 t
ORIGIN      
        1 tctgatccga atactcccgc agaatatcgg atcatcctcg gcgcagattt tcaaccgtgc
       61 cagcgtgccc gcatcggaac ttttccgcaa ccacccactg ctaccccgac actcactccg
      121 taaagacgac cgaagaccac agaccgaaga cggcagttga tcattttcga tcaccgtgtt
      181 atttctgaac gacgcgagct gatgagatac aacgaatcat ctgttgtctg aggtccatgg
      241 tctgcaatca cttattcatt ttgcattgag cattgcctgc tcgtcctgta taatcacacg
      301 gctcgcacct ttgatgagga tgctcagcca aaaatttaac gagatatgtt ttagatcatg
      361 acgagtccgt caagcaaaag caagtcaggc atcagttttt ttgagtggtt gctcattgtc
      421 cttatcgtcg ccgtcgtgct gctcttgagc ggtattttgg gtttaggcaa tctgccagcg
      481 gctgatccga ttgtgggtat ctcgacgcgc acggcaaccg cgacaccaac atcgacagtc
      541 acaccaacgc caacatttat tcccacctcc acatcaaccg caacaccgac caatacgccg
      601 acgccgactt cgccgccgcg tccatcgggc gcaatccttc cgctgatctt tgtcacagcc
      661 ggtgaagtaa taagcggcac acccgtcccg acgcagatgc cgtacatcga tcaacccgaa
      721 ggctcgatta acatcctgct gctcggcacc gatcaacggc ctaccagcgg cggttatcgc
      781 accgattcga ttttcgtcat cggcgtaaac cctcagcgac agtatgtcac catgctcagc
      841 attccgcgcg atctatgggt gtatgtgcca atcgaagaca agttcgatcg gatcaacacg
      901 gtcgatgcgt ggggtgagag ccacaaagtg ccgggcggca gcgcgagttt gatcaagcaa
      961 acgctgttat ataacctcgg catcccgatc gattattatg cgcggatcga tttttccggc
     1021 gtgaagcaga tcgtcgatcg ggtaggcggc gtggaggtgc tggctttctg tccgctgtac
     1081 gatgtattcc cggatgtgtc cgatgatcaa agcgacatca tcagcgatcc ggcgctgctg
     1141 gcgacggtgc tcaccggcac gatcgatatt ccgtcgcccg gcctgtatac gctcgatggt
     1201 aagcacgcgc tgtggttcgc ccgatcgcgt aaatctactg gtgacttcga tcgatcgcgg
     1261 cggcagcagc aggtcttacg cgcgatctgg gcgaagatca aagccaacgg tttgatctcg
     1321 cagctgccga gcttgtgggg cgatgtgacc tcgatcgtgc aaaccgatct cagcgtcaac
     1381 gatgtgggtt acctcgccac attcgcagcg cagctggatg attcacggat caagcagcgt
     1441 gccatcgata agctatcgac cgacgggttt gttgctgcga acggtgcaga agtgttggcg
     1501 attgcgcccg atcgggtttc agccgtgttg caggaagcgt tcgagccgcc gctgacgaat
     1561 gtaacgtcgc aagcatccgc gcaaattgaa gtgtggaatg ggagcggcca agcaaattgg
     1621 gatttgcttg cgatcaatcg cttacgatcg gaagggtttg ttgtgaccag ctacggcccg
     1681 gccgatcacg tttactccca gacgcaagtc attaacttca acaccacagt gaagggctcg
     1741 cgtagctctc aactagcgcg cttgttcaat gttcgctcgt cgtcgaattt gatcgatcaa
     1801 cctactgcga atagtccgat caactaccgc ttgatcgtcg gatcggattt caattcgtgt
     1861 ctgccgccgc ccgcgccggt gcagttcccg acgccgacac caacagcgtc ccccgtaccg
     1921 taacgactta caataaacga atgatccatc atagcgtgaa gggtaaagcc cgacgtggca
     1981 atctcgtcgg gctgatggaa aaacgatccg gagattgctt cgtgcctagc ggcgctcgca
     2041 atgatgacca aaaatgaaaa gaggagactc gatcgagtct cctctttttg ttgcaccata
     2101 cattcgatta cttcgccagc gtgtgatcgc agtagtaggt catggccgct tgcatgaagt
     2161 cagccatgtc tgtgcgatat ttgtcgtagg tcgcgcggaa ctctggatgt tcggcatacc
     2221 cttgtccaag gcctcgaaag atttcgggcg tgactgtata gaagttaccc acccaggcat
     2281 agtgccgtcc gatggcctgt tggaccagtc gattggcgat cggtttgtcc atcaacccgg
     2341 caagttcgcg cgtgatggca tcgccctcag ccttgatcgc agcccactgc tgtttcgaca
     2401 tcttacccac tcgctgctcg gactcttcga cgatcttcac atcatagagc tcgcgcactt
     2461 cccgcttgta gcgatcgatc tgttcctgag agaaaccctc atacagatca gcatcagtta
     2521 aactcatttt agcctccgat agtttgtcga tcgttgcatc gatcgttttc aagagacggg
     2581 tgagccgatc ggcgcgctgt gttagcagcc ccttgtgatc cttcagtgcc ttgacctgat
     2641 cgaacccggg tcgatcgaga atggcctgaa tctcactcag cggcacatcg agttctttga
     2701 agaagagaat ctgctgcaag cgcagcaaat ctttctcgcc atactgtcga tagcctaccg
     2761 ccgttcgaga ggtcggcttc agcaaaccga tctggtcgta gtggtgcagc gtgcgcacgc
     2821 tcaccccggc cagtattgcc aagtgccgaa ccgcgtaagt cgtcttcact ttgtctccgt
     2881 ggggatgact atacactatg acgtagcgtc agagtcaagg ggaaaattga aattggtcag
     2941 ctcattattc cgctcgatac ggcgcttcgg tctcgatatc gccgacgtat tccggcagat
     3001 ttgattggcc cagaatctgg cgaatcgcct gtatctcacc gatatgatac cagtagtgat
     3061 acgtcacgcg ccgcagcgcc gagccgattg attgcccgac cgcttttcca tctaccagca
     3121 actcggtcaa caatagttca gttgtcaacg aatcgagaaa cggatcggcg gctttcgtca
     3181 ccatgtgcca tatatcgagc atcgcgttga atgaaggcgt gctcattggt gcgccgaagg
     3241 cgaagtcggt tatcagctgc ggaaataaaa tcttgccctg tggccgcgtc agccacgatc
     3301 gctgttcatg ccacgccaga tgtcccaccg tccacccgat cgaattcatc tgcccgaagt
     3361 ggcgcgctcc atcctcttcg gatacgcctt ctatgccgcg cagccattcg gatcgcgtga
     3421 agtgaaattg atcgatcagc ggatgagtca ttcgcttgct cctaaagtgg atgtcgtt
//
LOCUS       DASRTJ010000654         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_290620,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000654 DASRTJ010000000
VERSION     DASRTJ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290620"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFF70_13245"
     CDS             complement(<1..758)
                     /locus_tag="VFF70_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZY45705.1"
                     /translation="MTYSKSAVLSGGLEAGGTKFVCMIASGPGDIRDEIRFATTTPDE
                     TIDRAIDFFKRVTTLRAIGVGSFGPIDLRRDSASYGFITSTPKPKWANTDLVGKLKAA
                     LNIPIAFDTDVNAAAYGEYRWGTLQNFDPGIYITIGTGIGGGVIVNRQPIHGLIHPEI
                     GHMFIPHDRSIDPFPGVCPFHQDCFEGLASGPALRSRWKQPAETLPIDHPAWKLEAKY
                     VAYALSNLIVSYSPQRIVLGGGVMQRQELFPMIRR"
     gene            934..1962
                     /locus_tag="VFF70_13250"
     CDS             934..1962
                     /locus_tag="VFF70_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZY45706.1"
                     /translation="MQTEINEQPSVLERFLKTESDSAIHLAREIALRDVNYVLIAARG
                     TSDNAARYAQYVFGAYNHLPVALATPSLYTLYAAPPQMKGALVIGISQSGRSPDIVSV
                     IEAGREQSRPTLAITNDPHSALARAAEWVLPLGVGEERAVAATKTYTTSLMALALLST
                     ALANDAERRAELDRVPELMRQTLAGVQSVLPLIERYRYIERSAVIGRGFNYATAFEIA
                     LKIKELTRVVTESYSSADFLHGPISVLEPGFPLILIAPSGRTLADLSDLAIEAKRRGA
                     ELIVIADDPKLLQQAQTPLPLPPNIPEWLSPLVAVLPGQLFARQLAVAKGFDADRPLG
                     LTKVTETL"
     gene            2419..3093
                     /locus_tag="VFF70_13255"
     CDS             2419..3093
                     /locus_tag="VFF70_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45707.1"
                     /translation="MNKKIKMPMLIGLLVLTVILAGCDRPSADNPLTDTTANTQSLAD
                     VSAQQTADAIGADNQAQPEGTSPAGTALPPVQPQATDAPVPTAIPAQSTSAPATTSGP
                     AQSTPGPSTTSTSTCGATYIVQPGDRFFSIGRACNVNPYAIAQANNIGAPYFIYPGQQ
                     LIIPGGNPGPQPTTTPGGNTYVVQPGDNLFRIALQFGKTMQAIAAANGLSNINLIFVG
                     QRLTIP"
BASE COUNT          704 a          840 c          850 g          699 t
ORIGIN      
        1 cgccggatca tcggaaataa ttcctggcgc tgcatcacgc cgccgcccaa tacaattcgt
       61 tgcggcgaat aactgacgat taagttggaa agtgcataag ccacatattt tgcttccaac
      121 ttccacgccg gatgatcgat cggcaatgtt tcggcgggtt gcttccaacg tgatcgcagg
      181 gctggcccag acgccaggcc ttcaaagcaa tcttgatgga acggacacac gccagggaac
      241 ggatcgatcg atcggtcgtg cgggataaac atgtggccga tttcaggatg aatcaagccg
      301 tgaatcggct gtcggttgac aatgacaccg ccaccgatac cggtaccaat tgtgatgtaa
      361 atgcccggat cgaaattttg cagcgtaccc cagcgatatt cgccataagc cgccgcgttc
      421 acatcggtat caaaggcgat gggaatgttc aaggccgctt tcaatttgcc gacgagatcg
      481 gtgttagccc actttggttt cggtgttgaa gtgatgaaac cataactggc cgaatcgcgc
      541 cgaagatcga tcgggccgaa ggaaccaacc ccaatcgcgc gcaatgtcgt tacgcgcttg
      601 aaaaaatcga tcgcgcgatc gatcgtttcg tcgggtgtgg tggttgcaaa tcgaatctcg
      661 tcgcggatgt cacccggccc gctggcgatc atgcacacaa actttgtgcc gccggcttcg
      721 aggccgcctg acaagactgc gctcttagag tatgtcatgt ttcgatctcc tgacatatga
      781 cccaaacttc cagcgataag ccgagaatac cataagtgta caaataagtg gaggcaatgc
      841 gccttgccta tcttcggtga agcctgttaa aattgccaat gatcattttg tggacagagt
      901 ttggcatgct aggagcagag ggtggattca atattgcaaa ctgaaatcaa cgaacaaccg
      961 agtgtcttag agcgcttctt gaagactgaa agcgactcgg cgatccactt ggcgcgtgag
     1021 atcgctctac gcgacgtcaa ctacgtgttg attgcggcgc gtggcacgtc tgataatgcc
     1081 gcccgatatg cgcagtacgt gtttggcgcg tacaatcatc tgccggtggc actggcgaca
     1141 ccttccttgt atacactgta cgccgcgccg ccacagatga aaggcgcact cgtgatcggg
     1201 atttcgcagt ccggccgctc gcccgatatt gtcagtgtga tcgaagccgg ccgcgagcaa
     1261 agtcggccga cgctggcgat tacgaacgat ccacattcgg cgctggcgcg ggccgctgag
     1321 tgggtactgc cgttgggggt cggggaagaa cgggcggtgg ctgccaccaa aacatatacg
     1381 acttcattga tggcgctggc tttgctctca acggcgttag cgaatgatgc tgagcgacgg
     1441 gccgaactcg atcgagtgcc ggagttgatg cgtcagactt tggccggtgt tcaatcggtg
     1501 ttgccgctca tcgaacgtta tcgttacatc gaacgcagtg cagtgatagg acgcggattt
     1561 aattatgcga ctgcgttcga gatcgcgctg aagatcaaag aactgacacg cgttgtgacc
     1621 gaatcctatt catcggcaga ttttctgcac ggcccgatct cggtgttgga gccgggcttt
     1681 ccgttgattc tcattgcgcc atcgggtcgg acgctggctg atctatccga cttggcaatt
     1741 gaagccaaac ggcgcggagc agaattgatc gtgatcgctg atgatccaaa actgttacag
     1801 caagcgcaga cgccactgcc attgccgccg aacataccgg agtggctatc gcccttagtc
     1861 gccgtactgc ccggccagtt gtttgccagg cagttggctg tggctaaagg cttcgatgcg
     1921 gatcggccat tgggcctgac gaaagtgacc gaaacgcttt aagcgccgag tggttgtgcg
     1981 tgctagatta ccatcagtga tgatgcggac aaaacaatct tcgataactg cagctgaacg
     2041 ccgacgccgc gctgatgtcg tacggctgat cattcttacg cgtgaaaagt ttccgcactc
     2101 gccgcgctgg atgaatgctt tgcgacgcgt cctgtggcgt tatgcggccg cagctccgcg
     2161 ctcggcgtct aaaaagaaca aacgctgaca gacattccgc gtgcgttcaa tagattcttt
     2221 gatcgattca caaccgatct tgtttgagca accgcttcag cgtctgaata atcatcgatt
     2281 gtttgctcgt ttctccgatc gatctcggcg ctttttcgct tgacctcccc agtgacttat
     2341 gctattctgt ggcttgagag taagcgagtg gctgaggaag cacttgatca aattcgccca
     2401 ccttaaggag gggcttcgat gaacaaaaaa ataaagatgc ccatgttgat tggtctgctg
     2461 gtcttgacag taattctcgc cggctgcgat cgacccagtg ccgacaatcc gttgacggat
     2521 actacggcga acacccagtc gttggcggat gtcagtgcac agcagacggc agatgcaatt
     2581 ggagcggata atcaagcaca accggaaggc accagtccgg ccgggactgc cttaccgccg
     2641 gttcaacctc aagccacaga cgcgcctgtg ccgactgcca taccggccca atcgactagt
     2701 gcgcccgcta cgaccagtgg tcctgcgcaa tcgacacccg gcccgtcaac aacttccaca
     2761 tccacttgcg gcgcaactta tatcgtccag cccggcgatc gattctttag cattggccgc
     2821 gcctgcaacg taaatccata cgccattgcg caagcgaaca acatcggcgc accgtatttc
     2881 atttatcccg gacaacagtt gattattccg ggtggcaacc ccggtcctca gccgacaacg
     2941 acgccgggtg gcaataccta cgttgtgcag ccgggcgata atctgttccg catcgcgttg
     3001 caatttggca agacgatgca ggcgattgct gcggcgaacg gcttaagtaa tataaatttg
     3061 atcttcgtcg gccagcggct gacgattcca taa
//
LOCUS       DASRTJ010000655         7988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_365105,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000655 DASRTJ010000000
VERSION     DASRTJ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365105"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VFF70_13260"
     CDS             <1..1430
                     /locus_tag="VFF70_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY45708.1"
                     /translation="SDRSFRAQRFSYILPFSIALLSLFILLPQSAEATPQANTRYVAK
                     SGSNSGTCSTSGTACMTVQYAVDQAVAGDAIYIAAGTYTSVTLRTGTYQQVYISKTLS
                     LQGGYTTTDNFAKSNPVANPTVLDAQGGGRVVYFNGVAGSVSNLSVQNGNFAGGDGGG
                     IYASNALTLTDVTVMSNTASNNGGGVYANTVATLNGGRFQNNTATTLDGGGLNVRSRL
                     VLRVTQFFSNTAGGNGGGLYLVNGINSSVQQARFRGNSAGDEGGGLFSINSAYVQLNA
                     NQITDNGAVNGGGGLYAATDNILALDNNLFAANTSTLGAAEIEIGGSGGPSVLIGRHN
                     TFAAASVGSGIAVNLGADVPGDRLVLTNTLFYGYNLALQTGPNAATIIAKAVLFDSVT
                     TQTQNNAGVISVTTIYSGSAAFVNASARNYHLQPGSAAINAGINAGVTTDIDGQTRPF
                     NGLYDIGYDEYVIYKIYLPLALKDF"
     gene            complement(1609..2751)
                     /gene="moeB"
                     /locus_tag="VFF70_13265"
     CDS             complement(1609..2751)
                     /gene="moeB"
                     /locus_tag="VFF70_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZY45709.1"
                     /translation="MTTELSHQEIRRYSRHLIMPEVGLEGQRKLKEAKVLLVGAGGLG
                     SPMAMYLAAAGVGHIGIMDFDVVDESNLQRQIIHGTSTLGQMKLDSAKNRMLDINPFI
                     DVTTYTEPLSSANALDIFKDYDIIADGTDNFQTRYLVNDACVLLGKPNVYGSIFRFEG
                     QASVFYAKEGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGVIGTIQATEVVKLIIGQG
                     DPLIGRLLLYDALEMSFTTLKLRKNPDCVICSDHPSVTHLIDYDQFCGVLPHAVEAQG
                     LEITVKELKAKLDGDDRNMILIDVREPHELEISKIDRAQLIPKNDLPDHLKELDSTKE
                     YVLLCKTGVRSLDAAKLMRAAGFRVKSVRGGINAWAREIDPSLPTY"
     gene            complement(2787..3098)
                     /locus_tag="VFF70_13270"
     CDS             complement(2787..3098)
                     /locus_tag="VFF70_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZY45710.1"
                     /translation="MPISINIPAPLRPYTGGQKIVRAEGRTVGEVLQALTAVHPNLRQ
                     HLFGEDGKLRSFINVYLNDEDVRYLQQGNTPIKAADTLSIVPSVAGGVNDLPLERSTR
                     E"
     gene            complement(3099..3533)
                     /locus_tag="VFF70_13275"
     CDS             complement(3099..3533)
                     /locus_tag="VFF70_13275"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZY45711.1"
                     /translation="MSLLHENRRIWQKHVLAENRRSVEGLLETLCNEPVYTIMATGEE
                     YRGKDAVAEFYTGLFAGVPDATFDPISVFVSEDGVMEESVLKGTQTGELFGLPPTHKS
                     FSLPLIIVFPIKNGEILGERLYFDMATLMHRLGIPLDQIPVT"
     gene            3695..4498
                     /locus_tag="VFF70_13280"
     CDS             3695..4498
                     /locus_tag="VFF70_13280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZY45712.1"
                     /translation="MAGKSRSNSRRANDMAVLRKPKPNASRSQVGCLILIAIAGTIGL
                     AVITTARAGGLPVQPIDTPRPAPTLTPTPSSFTASQPDGRPRRPTIGIVAGHWGNDSG
                     SVCPNGVSEQQTNLEIAKRVAELLSARDYRVDLLQEFDSRLNGYQADVLLSIHADSCD
                     IIDADPPATGFKVARSAASAIPNTEDTLVNCLTDNYASETKLPLHTNSITHDMTDYHS
                     FREVSLDTPAAIIETGFLKLDYNLIVKQPDIPARGIAAGITCFINSLPK"
     gene            4495..5589
                     /locus_tag="VFF70_13285"
     CDS             4495..5589
                     /locus_tag="VFF70_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZY45713.1"
                     /translation="MKTSRTSVDQLMDYGWLLIKDGQPAAARKFFEKIIEREPDHINA
                     LLLLAGVCDRGEDSLRYLARVLEIDPKSDRAHAGIRWARKRVHPYLAEGGDQVTQPIS
                     DLTVDQTPPDQADPDRAAQPELKNGQGTHPAAGLLLGGLIGLILIACLGVGAIWLFGP
                     PHNASAQIAPTIRPSATATLARTSTATSIPTATSTPTLAPTDIVAPAPTIEPTGVPTE
                     VPTAAPLPTDEPTIEPTLAPIDPPPPSSYQPRWIDVDLTNQMLTAYEGDTPVRTTLVS
                     TGLPGTPTVVGQFNIYVKYPAQLMVGPGYYLPDVPWVMYFYNDYGLHGTYWHNNFGHP
                     MSHGCVNLPTPEAEWLYNWASIGTLVNIHY"
     gene            complement(5901..>7988)
                     /locus_tag="VFF70_13290"
     CDS             complement(5901..>7988)
                     /locus_tag="VFF70_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZY45714.1"
                     /translation="GVARNLVESGYQGGIYFINPKADQILSHACYPSIEAAPDPIDLV
                     VVIVPASNVPQTLIDCGIRGVRSAIVMSGGFGETDEAGKQLERRIVEIAQQYHMRLIG
                     PNCIGLIDTHVPFNTTFIKSVPQPGEIAFVSQSGAICQAVIDWGTGMGFGFSRVASLG
                     NQADVSEAEVISALADDPNTRVITIYLEGVKDGGKFIEAATLATREKSVVALKVGRSV
                     SGKRAVSSHTGALAGHETAYDAAFDRCGVIRANNTEELFDWARALAWCPPMRGDRVAV
                     LTNAGGPGILAVDAIEAAGLKMATLTEETINALKSFLLPYASLHNPIDMLASAGPHEY
                     AESLHVLMHDPQVDAIVIVSVPPPIESPTPVAEAIAFVGRNTDKPIVVAVMGEATVGD
                     ALKTLRAVRLPDYRFPERAASSLGILWKQAQLRARSIDAPEKFSDVNRDSAQHLLDDA
                     LNSPRLMTELNADHRRTVSSSGEGPGVRLIVGSVATAILQEYKIFGPCEALARSADEA
                     VRWAQKFGFPVVLKVASSDIPHKSDIGGVALDLADEDSVRHAFDRIMANALKAKPDAH
                     IDGVTMQQMVKHGQEVIIGAVRDEQFGPLMMFGAGGIEVEGARDVAFGLAPLSRSEAE
                     KMIDATFAGRRLFGFRNIPPADREAVIKALLRLSQFVVDFPQVQEVEINPLRAQASGQ
                     GAIAIDVRISMKS"
BASE COUNT         1812 a         2375 c         2102 g         1699 t
ORIGIN      
        1 aatccgatcg atcattccgc gcccaacgct ttagctatat tctgcccttc tccatcgcct
       61 tactcagtct cttcattcta ttgccacagt cagctgaggc gacaccgcaa gccaacactc
      121 gttacgttgc caagtcaggc agcaatagtg gcacctgctc gaccagcgga acggcctgca
      181 tgaccgtgca atacgcggtt gatcaagcgg tggctggcga tgcgatttat attgcggctg
      241 gcacatatac cagcgtgacc ttacgcactg gcacctatca acaggtctac atcagcaaga
      301 cgctttcgct acaaggcggc tacacaacga cggataattt cgctaaatcc aaccccgtcg
      361 ccaacccgac cgtcctcgat gcgcaaggtg gcgggcgcgt cgtatacttc aacggtgtgg
      421 ctggcagtgt ctccaacctc agcgtacaga atggcaactt cgccggcggc gacggcggtg
      481 gcatctacgc ctcgaatgcg ctgacactga ccgatgttac cgtcatgagc aatacggctt
      541 ccaataacgg tggtggcgtg tatgccaaca ccgtagcgac cctgaatggt gggcggttcc
      601 agaataacac cgctactacc ttggacggcg gcgggctgaa tgtgcgtagc aggctcgtgc
      661 tgcgtgttac acagttcttc agtaacacag caggcggcaa cggcggcggc ctttatctag
      721 taaacggcat caactccagc gtgcagcagg cgcgcttccg gggcaatagc gcgggcgacg
      781 aaggcggtgg cctgttcagc atcaattcag cctacgtgca attgaacgcc aatcagatca
      841 ccgataacgg cgcggtgaat ggcggcggcg gcctatacgc cgccacagac aacatcctcg
      901 ccttagacaa caatctcttt gcggcgaaca ccagcacgct gggcgcagcc gaaatcgaaa
      961 ttggcggttc gggtggtccg tctgtgctga tcgggcggca caacacgttc gccgcggcga
     1021 gcgttggtag cggcatagcg gtcaacctcg gcgcagatgt gccaggtgac agactcgtcc
     1081 tgaccaacac gctgttctac ggctacaacc tcgcgctgca aacaggtccg aatgcagcga
     1141 cgatcatcgc caaggccgtg ctcttcgaca gcgtgaccac gcagacgcag aacaacgcgg
     1201 gcgtgatcag cgtgaccact atctattcgg gcagcgcggc ttttgtcaat gcgagcgcgc
     1261 gcaattatca cttgcaaccc ggcagcgcgg cgattaatgc cggtatcaat gcgggcgtga
     1321 ccaccgatat cgatggacaa acgcgtccgt tcaatggact gtacgacatc ggttacgatg
     1381 aatatgtaat ctacaagatc tatctgccgc tggcgttgaa ggatttctag ttcactctac
     1441 acatcgtcat tgcgagccgc agcagcggcg aagcaatctc aacgacaatc aactgagatt
     1501 gcttcgtcag cctaacggcc ttctcgcaat gacgacctaa caatgaaaag aggccgcgca
     1561 tttaatgcgt ggccactttt tgatccattg gctttgttgt gagaggaatc agtacgtcgg
     1621 caaactcgga tcgatctcgc gcgcccaggc gttgatgccg ccgcgcacac tcttcacgcg
     1681 aaagcccgcc gccctcatta acttggccgc gtccaaagat cgaacgccag tcttgcacag
     1741 cagcacatac tctttggtcg agtccaattc tttcagatga tccggcagat cgttcttcgg
     1801 gatcaactgc gcccgatcga tcttcgagat ttccagttcg tgcggctcgc gcacatcgat
     1861 caagatcatg ttccggtcat cgccatccag cttggctttc aattctttga ccgtgatctc
     1921 caaaccctgc gcctcgactg catgtggcag gacgccgcag aattgatcgt aatcgatcaa
     1981 gtgggtgacg ctgggatgat cggagcagat cacgcaatcg ggattcttgc gcagcttcaa
     2041 tgtagtgaaa ctcatttcga gcgcatcata cagcaacaat cgcccgatca acggatcgcc
     2101 ctgcccgatg atcaacttga cgacttcggt cgcttgaatc gtaccgatca cgccgggcag
     2161 cacgcccagc acgccacctt cagcgcatga cggcaccagt ccgggcggcg gcggctcagg
     2221 atacaagcag cgatagcacg gcccttcttt ggcatagaag accgaggctt gtccttcgaa
     2281 gcgaaagatg ctgccataca cattcggctt acccaataac acacacgcat cattcactaa
     2341 gtaacgcgtt tgaaaattat ccgtgccatc cgcgatgatg tcgtagtctt tgaagatgtc
     2401 gagcgcattc gcggacgaga gcggctctgt gtacgtcgtg acatcgatga acggattgat
     2461 gtccagcatc cggtttttgg ccgagtccaa tttcatctgg cccagcgtcg atgtgccgtg
     2521 aatgatctgt cgctgcaagt tgctttcatc gacgacgtca aagtccatga tgccgatgtg
     2581 gccgacgcct gccgccgcta aatacattgc catcggtgaa ccgagtccgc ccgcaccaac
     2641 gagcaggact ttagcctctt tcaatttgcg ctggccctcc agcccgactt caggcatgat
     2701 caagtggcgg ctgtagcgcc ggatctcttg atggcttaac tcagtcgtca tttgtcgtcg
     2761 cctcgatgat tcaacgcgca gtccattcat tcgcgggtac ttcgttccaa gggcaagtcg
     2821 ttgacaccac ccgccacaga cggcacgatg cttaaagtat ccgcagcctt gatcggcgtg
     2881 ttgccctgtt gcaagtagcg cacatcctcg tcattcaaat aaacattaat gaatgatcga
     2941 agtttaccat cttcgccaaa tagatgctga cgcaaattgg gatgcaccgc cgttaacgcc
     3001 tgcagtactt cgccgacggt gcggccttcg gcgcgcacga ttttttgtcc acccgtataa
     3061 ggtcgcaacg gggctggaat gttaatcgag atgggcattt acgtgaccgg aatttgatcg
     3121 agcgggatgc cgagtcgatg catcaacgtg gccatatcga aatacaatcg ctcgccgagg
     3181 atctcgccgt tcttgatcgg gaacacaatg attaatggga gcgagaaaga tttatgagtc
     3241 ggcggcaagc cgaacaattc gcccgtctgg gtgcccttca acaccgattc ttccatcaca
     3301 ccatcttcag acacaaagac gctgatcgga tcgaaggtgg catcaggcac accggcaaac
     3361 agtccggtat aaaattcggc gaccgcatcc ttgccgcgat attcttcgcc cgtggccatg
     3421 atcgtataga ccggctcgtt gcacaaagtt tccagtaagc cctcaacgct gcgccggttt
     3481 tccgccagca catgcttctg ccatatccgt cgattctcgt gcaacagcga catgaatgtt
     3541 ctcctgcaaa taaagaagtg tcagaccgtc atcatcatag cacaatttcc aggcggttcg
     3601 cagatttctg cccaggttct gtcgaaattc atgcccacgt gccaatcgca gcgcgttttt
     3661 gacttggttt gtattcagaa gtatcataca ccgcatggct ggaaaaagcc gatcgaactc
     3721 acgccgcgcg aacgacatgg ccgtgctgcg taaacccaaa ccaaacgcct cccgatcgca
     3781 agtgggctgc ctgatcttga tcgccattgc cggaacgatc gggttagccg tgatcaccac
     3841 cgcacgcgcg ggcggcttgc ctgtccaacc gatcgacact ccgcgacccg ccccaaccct
     3901 cactccaacg ccatcgtcat tcactgcatc tcaaccagat ggccggccgc gccgaccgac
     3961 gatcggcatt gtcgctggac attggggcaa cgactccggc tcagtgtgtc ccaacggtgt
     4021 ctctgagcag cagaccaatt tggaaattgc caaacgtgtc gccgagcttt taagcgcgcg
     4081 cgattatcgc gtcgatctct tgcaggaatt tgattcgcgc ttgaacggtt atcaagccga
     4141 tgtgctactg tcgatccatg ccgattcatg tgatatcatt gacgccgatc cacccgccac
     4201 cgggtttaag gtagcccgat cggccgccag cgcgatcccg aacaccgagg acactctggt
     4261 caactgcctc acagacaact atgctagcga aacgaaattg ccacttcaca cgaacagcat
     4321 cacccacgat atgaccgact atcattcttt ccgcgaagtg agtctggaca cgccggcagc
     4381 gatcatcgag acgggattct tgaagttgga ctataatctg attgtgaagc aaccggacat
     4441 tccggcgcgt ggcatcgccg ctggaattac ctgctttatc aacagtctgc cgaaatgaaa
     4501 acatcacgaa ccagcgtcga tcaattaatg gactatggct ggctgctgat caaagacggt
     4561 cagccggctg ccgcgcgcaa gttctttgag aaaatcatcg aacgcgagcc cgaccatatc
     4621 aacgcgctct tgctgctggc cggtgtgtgc gatcgaggcg aagacagcct gcgctatttg
     4681 gcgcgcgtgc tggagatcga tccgaagagc gatcgagcac atgctggtat tcgctgggca
     4741 cgcaagcggg tgcatcctta tctcgctgaa ggcggcgatc aagttacgca gccgatctct
     4801 gacctgacgg ttgatcaaac gccgccggat caagctgatc ccgatcgcgc cgcccaacct
     4861 gaattgaaaa atggacaggg cactcacccg gccgcaggcc ttttgcttgg cggcttgatc
     4921 ggtttgatct tgatcgcgtg cctcggcgtc ggcgcgatct ggctgtttgg tccgcctcac
     4981 aatgccagcg cacaaatcgc gccaacgatc aggccgagtg ccacggccac attagcgcga
     5041 acttcaaccg ccacatcgat ccccaccgca acatccacac cgactctcgc gccgacagac
     5101 atcgtcgcgc cagcaccaac gatcgagcca accggagtgc cgacggaggt tccaaccgca
     5161 gcgccactgc caacagatga accaacgatc gagccaactc tcgcaccgat cgatccgccg
     5221 ccaccttcga gctatcagcc gcgttggatc gatgtcgatc tgaccaatca aatgttgact
     5281 gcgtatgaag gcgacacgcc ggtgcgcacc acgctggtct cgaccggttt gcccggcacg
     5341 ccgacggtcg ttgggcaatt caacatctac gtcaaatatc ccgcgcagtt gatggtcggg
     5401 ccgggttatt atttgccgga tgtgccgtgg gtgatgtatt tctataacga ttacggtctg
     5461 catggcacgt attggcacaa caacttcggt caccccatga gtcacggctg cgtgaatttg
     5521 ccgacgcccg aggcagagtg gttatataac tgggcaagca tcggtacgct cgtgaatatt
     5581 cattattgat cacgatggct tagaaaccaa aacaaaagga tcgaggttaa gcgctaacct
     5641 cgatcctttt tattcgtgtt gattcgtgtt cattcgtgga aaaaaattcc tgcgccgatt
     5701 aattcaacac cagcggcaag taaactttaa actgtacaac gttgatcgta agcattttta
     5761 gatcgcaacc gaaggaactg ttcgccgtga gtgtcaggat cgaggtggtg gtgaccaggc
     5821 agacccgcga cgccaagtgt gatcggcgca atgccggtga tatcgattcc ataatgggca
     5881 gcagttaaac tgctgcccaa ttaagacttc atgctgatcc gcacatcaat cgcgatggcg
     5941 ccctgcccgg acgcttgagc gcgcagcgga ttgatctcaa cttcctgcac ttgcggaaag
     6001 tcgacgacga actgcgacag gcgcagcagc gctttgatga ccgcctcccg atcggccggc
     6061 ggaatgttgc gaaagccgaa cagtcgtcgt ccggcaaaag tcgcatcgat cattttttct
     6121 gcctccgatc gagacagagg ggcgagtccg aacgcgacat cgcgcgcgcc ttccacttcg
     6181 atgccgcccg cgccgaacat catcaacggg ccgaactgct cgtcgcgcac tgcaccgatg
     6241 atcacttcct gtccatgctt gaccatctgc tgcatcgtca caccatcgat gtgcgcgtca
     6301 ggcttggcct tcaatgcatt cgccatgatg cgatcgaacg cgtggcgcac cgagtcttca
     6361 tccgccagat cgagcgcgac accgccaata tccgatttgt gtggaatatc agacgaggcg
     6421 actttcaaca caacgggaaa accaaatttc tgcgcccaac gcacggcttc gtcggcggat
     6481 cgggccagcg cttcacaggg tccaaaaatt ttgtactctt gcaagattgc agtcgcgact
     6541 gagccgacga ttagcctcac ccccggcccc tcgcccgatg acgatacagt tcgacgatga
     6601 tccgcattca attctgtcat caggcgaggg gagttgagcg catcatctaa gagatgctgc
     6661 gcactatccc gattcacatc agaaaatttt tctggcgcat cgatcgatcg ggcgcggagt
     6721 tgggcttgct tccacagaat gcccaacgaa gacgcagcgc gttcaggaaa tcgatagtca
     6781 ggcaagcgca cggcgcgcag cgtcttgagg gcatcgccga ctgtggcttc gcccatcacc
     6841 gcgacgacga tcggcttatc cgtgttgcgg ccgacgaagg caattgcctc ggccaccggt
     6901 gttggcgact cgatcggtgg cggcacactc acgatcacga tcgcatcgac ctggggatcg
     6961 tgcatcaaga catgcagcga ctcagcatat tcgtgcggtc cggcagaagc cagcatatcg
     7021 atcgggttgt gtaaggacgc atagggtagc aaaaaacttt ttaaagcgtt gatcgtttcc
     7081 tcagttaaag tggccatctt caatccggcc gcttcgatcg catcgacggc gagaatgccg
     7141 ggaccgcccg cattggtgag aacggccact cgatcgccgc gcatgggcgg acaccatgcc
     7201 aacgcgcgcg cccaatcgaa taactcttca gtgttgttgg cccgaatcac gccgcaccga
     7261 tcgaacgcgg catcatacgc ggtctcgtgt ccggcaagcg cgcccgtgtg cgatgacacg
     7321 gcgcgtttac ctgaaaccga tcggccgact ttcaaggcga cgacgctttt ctcgcgcgta
     7381 gccagcgtcg ctgcttcgat aaattttccg ccgtctttca cgccttccaa atatatggtg
     7441 atgacccgcg tgttgggatc atcagccaac gcgctgatca cttcggcctc ggacacatcg
     7501 gcttgattgc ccaacgacgc cacgcgtgaa aaaccgaagc ccatgcccgt gccccaatcg
     7561 atcacggcct gacagatcgc gccggactgc gacacaaacg caatctcgcc cggctgcggg
     7621 acactcttga tgaatgtcgt gttgaacggc acatgtgtat cgatcaagcc gatgcagttt
     7681 ggtccgatca aacgcatgtg atattgctgc gcgatctcga cgatccgcct ttcgagttgt
     7741 ttgccagcct catcggtttc gccgaatcca ccggacatca caatagccga tcggacgccg
     7801 cgtatcccac aatcgatcag cgtctgcggc acattcgagg caggcacaat tactaccacc
     7861 agatcgatcg gatcgggtgc ggcttcgatc gagggataac aagcgtgact gagaatttga
     7921 tcggctttgg gattgatgaa gtagatgccg ccttgataac cggattcgac caagttacgc
     7981 gcgacccc
//
LOCUS       DASRTJ010000656         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_365182,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000656 DASRTJ010000000
VERSION     DASRTJ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365182"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFF70_13295"
     CDS             <1..833
                     /locus_tag="VFF70_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45715.1"
                     /translation="AQADRPVAVVKCVDDFEAAFKSKHYSDRTYTVGRVNSVGLTKKN
                     IVDMQAWEPAEFSPPAEFHYTTAQEAAHIYYDLVKPIWSLNPVVDAWETFNEFSWHWG
                     WQADFYIQLMDLAEADGFRLGLWSPSGGNPPEEFYPEIARACRRAKAHGDHILCLHEY
                     SWDGLLKEAPSSLVTRYRRLYKYLKEVDAVIPLVISEAGENGGGGFTGTPTFIDDFAW
                     YDAQLSRDSYVIGCAAWTLGDWSGANFQTALPALADYITGQHDPLSSKIYLPAIQIDR
                     "
     gene            complement(1121..1384)
                     /locus_tag="VFF70_13300"
     CDS             complement(1121..1384)
                     /locus_tag="VFF70_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45716.1"
                     /translation="MLFALLFFDLGLLDQTCQIKIGYRWIVSEIGGGKLIRCARAVPP
                     DVAIIDQLAAERHMIQTKVHAEMSSPGLKLIIEFAFRIQSIWG"
     gene            1690..2091
                     /locus_tag="VFF70_13305"
     CDS             1690..2091
                     /locus_tag="VFF70_13305"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HZY45717.1"
                     /translation="MSTQAKATFEVKTWDEKPYAEFGGKAKLTRASVTKTFHGDIEGE
                     GTLEYLMAYTEDGSAGFVGMEHIVGRIGNRSGSFVLQHSGSDDGSTSKVDLLVVSNSG
                     TGDLYGLRGHGSSVISRDQPQYAFTLDYDFE"
     gene            2174..2593
                     /locus_tag="VFF70_13310"
     CDS             2174..2593
                     /locus_tag="VFF70_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY45718.1"
                     /translation="MTQLSPYLKFDGNCHEAMTFYQKCLGGELSLQAVEETPMAAQMP
                     AEAQKKILHATLTHGGIVLMASDMVGPEGLVHGSTIFLSLDCGSEEEIKTFFSKLSAG
                     GQVTYPLKTEFWGATFGQVIDKYGMTWLLNYTQNPPE"
     gene            2661..3872
                     /gene="recA"
                     /locus_tag="VFF70_13315"
     CDS             2661..3872
                     /gene="recA"
                     /locus_tag="VFF70_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZY45719.1"
                     /translation="MARKKKAESTEELTDFIEGAKTKKKSKEDKKPEIDPGKSRALET
                     TLSTILKRFGEGSIMRLGEATHMQIETIPTGSLALDLALGVGGIPKGRVTEIYGPESA
                     GKTTLCQHIVAEVQKRGGICAYIDMEHALDPNYAAVCGVDIDNLYISQPDTGEQALEI
                     AESLVRSNAVDLIVIDSVAALVPRAEIEGEMGDSHMGLQARLMSQALRKLSGAIKQSN
                     TALVFTNQLRMKIGVMFGNPETTTGGNALKFYASVRLDIRRTQSIKTDAEVVGNRVKV
                     RVTKNKVAPPFREAEFDIMFNEGISKVGDILDIGANMEIITKRGAFFAYGDVKLGQGR
                     ENGKDFLKANPAIANEVEAAIRGNVAKAAELAKASLRHAPDEIEADEEDTPEGAVAIA
                     AAVVEEAESEL"
BASE COUNT          993 a         1051 c         1006 g          838 t
ORIGIN      
        1 gtgctcaggc cgatcgaccg gtggctgtgg tcaaatgcgt cgatgatttc gaagctgctt
       61 tcaaatccaa gcactacagc gatcgaacgt acaccgtcgg gcgtgtcaat tcggtcgggt
      121 tgaccaagaa aaacatcgtc gatatgcagg catgggaacc cgctgagttt agtccgcccg
      181 ccgagtttca ctacaccacc gcgcaagaag ccgcgcatat ttattacgat ctcgtcaagc
      241 cgatctggtc gctcaatccc gtggtcgatg cctgggaaac gttcaatgaa ttctcgtggc
      301 attggggctg gcaagctgat ttctatatcc aactgatgga ccttgctgaa gccgacggtt
      361 ttcgtttggg cttatggtcg cccagtggcg gcaatccgcc ggaagagttt tatcctgaaa
      421 tcgcccgcgc ttgtcggcgc gctaaagcgc acggcgatca cattttgtgc ctgcacgaat
      481 acagttggga tggattgttg aaagaagcac catcgagttt ggtcacacgc tatcgacggt
      541 tgtacaaata tttgaaagag gtcgatgccg ttattccact cgtgatctcc gaagcaggcg
      601 aaaatggcgg cggcggattc accggcactc caaccttcat cgatgatttc gcctggtatg
      661 atgcgcagct cagccgcgac agttacgtga tcggttgtgc ggcgtggaca ctgggcgatt
      721 ggagtggcgc gaattttcaa accgccctgc ccgcgttagc cgattacata accggtcagc
      781 acgatccgtt atcctcgaaa atttatctgc cagcgattca gatcgatcgg tgagtctgaa
      841 cggtctggtt cactccgagt gctttgcctg cgcgatcgag ttttcacgcc cgttaggcca
      901 tttcacatat cggcatccta acaatcccga tcggattgac tttcgctcag ccgtgggttt
      961 atactattgt ccgattttat aaattttgtc tggaggaact atgctccaca tcactatcga
     1021 tcaatttata aatcgttttg tggccttagt tatggccgga ggtggtctgc ccacgaaacc
     1081 acaagatcga cagatcctat acctgagttc aattctcaaa ttagccccaa atcgattgta
     1141 ttctgaaagc gaactcaatg atcaacttca accctgggct gctcatttcg gcgtgaactt
     1201 tggtttggat catgtgacgc tccgccgcta gctgatcgat gatcgctaca tccggcggga
     1261 cagcgcgggc acatcgtatc aacttgccgc cgccgatctc acttacgatc catcgatagc
     1321 cgatcttgat ctggcaggtt tgatcgagca agccaagatc gaaaaagaga agcgcaaaca
     1381 gcaattcatg caaaacgcca agcgctaaat ttgaactcct tcccgatcta tgccaattgt
     1441 agttgcaatt gggtggctcg ctaagccatc gccatccgcc gattctcaat ggaaggacca
     1501 actccaccct agtccaacaa aggcctcagg aagtctctta tgaatagctg gtaagcgtcc
     1561 caaatcccgg ttccggatac ccaatcccta atccctattt tttaaggttg tgttcgattg
     1621 acaagataga acgcttgtgc tattctaaca gtagcctaat atcagcaaca attgctggag
     1681 gaaatgggga tgagtacaca agcaaaagct acgttcgaag taaaaacttg ggatgagaaa
     1741 ccttacgcgg aattcggcgg caaggccaaa ctcactcgcg ccagtgtgac caaaacgttc
     1801 cacggcgaca tcgagggcga aggcacattg gaatatctga tggcctatac tgaagatggc
     1861 tctgctggtt ttgtcggtat ggaacacatt gtcggccgta tcgggaaccg atcgggcagc
     1921 ttcgtcctgc agcatagcgg tagcgatgac ggcagcacat cgaaggtcga cctgctcgtc
     1981 gtgtcaaact ccgggactgg cgatctgtat ggcttgcgcg gccacggcag tagtgtcatt
     2041 tcccgcgacc agccacaata tgcctttacg ctcgactatg attttgagta aaatgcattc
     2101 gagacgatgg attgcacgga ctaacttcta gcccaggccc aacatcaatc atttactgcc
     2161 aaggagaatc aagatgacac aactgagtcc gtatttgaaa ttcgacggca actgccatga
     2221 agcaatgacc ttctaccaaa agtgcttggg cggagaactg tcgctgcaag cggttgaaga
     2281 gactcctatg gccgcccaga tgccggctga agcgcagaaa aagattctgc atgccacatt
     2341 aacccatggc ggtatcgtgc tcatggcttc ggatatggtg gggccggagg gactcgtcca
     2401 cggcagcacc attttcctgt cgctcgattg cggcagcgaa gaagaaatca aaacattctt
     2461 ttcaaaactt tccgctggcg ggcaagttac ctacccattg aaaacggaat tttggggcgc
     2521 gacctttggt caagttatcg acaagtatgg gatgacctgg ttgctaaact acactcagaa
     2581 cccgccagaa taatacgcaa taagtcgcat gaatcttggc gaccatacaa tcacaccaca
     2641 ttatcatcaa ggagaacaac atggcacgca agaagaaagc cgaatcgacc gaagaactaa
     2701 ccgacttcat cgaaggggcc aagaccaaga agaaaagcaa agaagacaag aaaccggaaa
     2761 tcgatccggg caagtcgcgc gcgctcgaaa ccactctcag cacgatcttg aaacgcttcg
     2821 gcgaaggatc gattatgcgg ctaggcgaag ctacgcacat gcaaattgag accatcccga
     2881 ctggctcact cgcgctcgat ctggcgcttg gcgtgggcgg cattcccaaa ggccgcgtca
     2941 ccgagatcta cggccccgaa tcggccggca aaaccacgct ctgccagcac atcgtcgccg
     3001 aagttcagaa gcgcggcggc atctgcgcct atatcgacat ggagcacgcg ctcgatccca
     3061 actacgcggc cgtgtgcggc gtcgatattg ataatctcta catctctcag cccgacaccg
     3121 gtgaacaagc gctggaaatc gccgagagtc tggtgcgatc gaatgcagtc gatctgattg
     3181 tgatcgattc cgtcgccgcg ctcgtgccac gcgccgagat cgaaggcgag atgggcgact
     3241 cacacatggg tctgcaagcc cgattgatgt cgcaggccct gcggaagttg tccggcgcga
     3301 tcaagcaatc gaacaccgcg ctggtgttca ccaaccaact gcgcatgaag atcggcgtca
     3361 tgttcggcaa tcccgaaacg accaccggcg gcaacgcgct caagttctac gcttcggtgc
     3421 gattagatat tcgccgcacg caaagcatca agaccgatgc ggaagttgtg ggcaatcgcg
     3481 ttaaggtgcg cgtcaccaag aacaaagtcg ccccgccctt ccgcgaagcc gaattcgaca
     3541 tcatgttcaa cgaaggcatc agcaaagtcg gcgacattct cgacatcggt gcaaatatgg
     3601 aaatcattac caagcgcggc gcgttcttcg cctatggcga tgtgaaacta ggccaaggcc
     3661 gcgagaacgg caaggacttc ctgaaagcta acccggctat cgccaacgaa gtcgaagccg
     3721 ccatccgagg caacgtggcc aaggcagcag aattagccaa agccagcctg cgtcacgctc
     3781 ccgatgaaat cgaagccgat gaagaagata cacctgaagg cgcagtcgcc atcgctgcgg
     3841 ctgtcgtcga agaggccgaa agcgaactgt aaaaaaactc tgtgacac
//
LOCUS       DASRTJ010000657         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_210181,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000657 DASRTJ010000000
VERSION     DASRTJ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210181"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VFF70_13320"
     CDS             complement(<1..1595)
                     /locus_tag="VFF70_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZY45720.1"
                     /translation="MRYGHFDDEQREYVITQPDTPVPWINYLGCENYFGLISNTAGGY
                     SFYRDARLRRLTRYRYNNAPLDLGGRYIYLRDNASQQFWSPTWQPTRRELTNYTCRHG
                     LGYTTISSTYQDIEARALYFVPLGETLEVWQLTLTNHRSTPAHLSVFSSIEFCLWDAQ
                     DDATNFQRNFNTGQVEVADRVIYHKTEYRERRDHFAYFACSEKLAGFDTQRESFLGAY
                     RGWDSPVAVERGESFNSIAQGWQPIGSHHIQITLAPNETRRIIFVLGYHENPRDQKFD
                     PPGSQTINKRTVKPIIAKYLEAAQVAAAFESLRKYWNDCLAVFQVNTPDVHTNRMVNI
                     WNPYQCMITFNLSRSASQFESGIGRGMGFRDSNQDLLGFVHMIPARARERILDIAATQ
                     LESGGAYHQYQPLTKRGNNDVGSNFNDDPLWLILGVSAYLKETGDWSILDELVTYDNR
                     SGSEQPLLDHLQRSYRYTIERLGPHGLPLIGRADWNDCLNLNCFSEEPGESFQTTTNR
                     DGKVAESIFIAGLFVQAANELAA"
     gene            complement(1810..>2470)
                     /locus_tag="VFF70_13325"
     CDS             complement(1810..>2470)
                     /locus_tag="VFF70_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZY45721.1"
                     /translation="RAVAREAVAKSLVLLKNDQSALPLSKSAPLIYVAGIGANDIGMQ
                     SGGWTIEWQGQLGNITPGTTILKAITSTVKAGTVVKYDAEGNFEGGKVADACVAVVGE
                     QPYAEGRGDKEDLSLAPADVQMTERLRDKCQKLIVVLISGRPLIVTEPLKQWDAFVAA
                     WLPGTEGQGVADVLFGDKPFTGKLPYTWPRSNDQLPFDFKNLGSGEADPLFPFGFGLS
                     R"
BASE COUNT          556 a          699 c          669 g          546 t
ORIGIN      
        1 atagccgcga gttcattcgc cgcctgcaca aacaggccgg caatgaatat cgactcggcc
       61 actttgccat cgcgattggt cgtcgtttga aaagactcgc ccggttcttc ggagaagcag
      121 ttgaggttga ggcaatcatt ccaatcggcg cgaccgatca agggtaagcc gtgggggccg
      181 agccgctcga tcgtgtaccg atagctccgc tgtaagtgat ccaacaacgg ctgttcagaa
      241 cccgatcggt tatcgtacgt caccagttcg tcgaggatcg accaatcgcc cgtctcttta
      301 agatacgcgg atacacccag aatcagccac aaaggatcat cattgaagtt gctgccgacg
      361 tcgttgttgc cgcgcttggt cagcggttga tattgatgat acgcgccgcc ggactccaac
      421 tgcgtcgcgg cgatgtcgag aatgcgttct cgcgcgcgcg cgggaatcat gtgcacgaag
      481 ccgagcaaat cttgattcga atcccggaag cccatgccgc gcccaatgcc tgattcaaac
      541 tgcgaggcgg agcgcgaaag gttgaacgtg atcatgcact ggtaagggtt ccagatgtta
      601 accatgcgat tggtgtgaac atcgggcgtg ttgacttgaa agaccgccaa gcaatcattc
      661 cagtatttcc ttaaactctc gaaggccgcc gcgacttggg cggcttcaag atacttggcg
      721 atgattggtt tgaccgttcg tttgttgatc gtctgtgaac cgggcggatc gaatttctga
      781 tcgcgcggat tctcgtgata gcccaacacg aaaatgatcc gccgcgtttc attcggcgcc
      841 agcgtaatct ggatgtgatg tgaaccgatc ggctgccaac cctgcgcgat cgagttgaac
      901 gattcaccgc gctcgaccgc gacaggacta tcccagccgc gataagcgcc caggaacgac
      961 tcgcgctgcg tatcaaatcc ggccaacttc tcggagcagg caaagtaggc aaagtgatca
     1021 cgccgctcac gatactcggt cttgtgatag atcactctgt ctgctacttc aacttgcccg
     1081 gtgttgaagt tgcgttgaaa gttcgtcgca tcgtcctgag catcccataa acaaaattcg
     1141 atgctggaga aaaccgagag gtgagccggg gtcgagcgat gattagtgag cgtgagctgc
     1201 cagacttcga gcgtttcacc caacggcacg aagtacaagg cgcgcgcttc gatgtcttga
     1261 taggtcgagc tgatggtggt gtaacctaag ccgtgacggc aggtgtagtt tgtcaactca
     1321 cggcgggtcg gctgccaggt tggcgaccag aactgttgtg aagcgttatc ccgtaaatat
     1381 atgtagcgtc cgcccagatc gagcggcgcg ttgttgtaac gatagcgtgt caagcgccgc
     1441 agtcgcgcat cccgataaaa ggaataacca ccggccgtat tggagatgag gccaaaataa
     1501 ttttcgcagc cgagataatt gatccacggc acaggcgtgt ctggttgagt aataacgtac
     1561 tcacgctgct cgtcgtcaaa atgtccatag cgcatgatcg attcaattgc tccatctaaa
     1621 ctcgaagtga caccacttcg attatttcaa gggctggctg attattcagg ccacccacat
     1681 atcgtacgta aacaacgtcg tcacatgccc agcaacaaca cactcgccag cgttcaccat
     1741 gcgcaacaca caaacactat tgcttcgccc gtgttcgatg acggacgaag cggacaggta
     1801 atgatctgtc taacgactta gaccaaatcc gaaggggaag agtggatccg cttcgccgct
     1861 gcccaagttc ttgaaatcaa atggcagctg atcgttcgac cgcggccacg tgtagggcag
     1921 ttttcccgtg aaaggcttat cgccgaacag cacatcggcc acgccttgtc cttccgtgcc
     1981 gggcagccac gccgcgacga aagcatccca ttgtttcagc ggctcggtga cgatcagtgg
     2041 tcggcctgag attaagacca cgatgagctt ctggcactta tcgcgcagac gctcggtcat
     2101 ttgcacatca gccggagcga gggacaaatc ttctttgtcg ccgcgacctt ctgcataagg
     2161 ctgttcgcca accacggcca cacacgcatc ggcgaccttt ccgccttcaa aattaccttc
     2221 cgcatcatac ttgaccaccg tgcccgcctt gaccgtgctg gtgatggctt tcagtatcgt
     2281 cgtgcccggc gtgatgttgc ccagctgccc ctgccattcg atcgtccacc cgccggactg
     2341 catgccgatg tcattggcgc cgatacccgc cacatagatc agcggcgctg atttggacag
     2401 cggcagtgcg ctctgatcgt tcttgagcag caccagcgac ttcgctactg cttcgcgtgc
     2461 tacggcgcga
//
LOCUS       DASRTJ010000658         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_137355,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000658 DASRTJ010000000
VERSION     DASRTJ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137355"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFF70_13330"
     CDS             complement(<1..390)
                     /locus_tag="VFF70_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45722.1"
                     /translation="MKVAVVGPTYPHRGGIAQYTSLLAFHLSQQCELRLYGFKQQYPN
                     WLFPGRSQIDPSPKPLFDIEVRRWLIPWWPLSWRRVVKDWQTWKPDRVVIQWWVPFMA
                     PMTASLASWARRHGIPTIFICHNVLPHE"
     gene            complement(506..1528)
                     /locus_tag="VFF70_13335"
     CDS             complement(506..1528)
                     /locus_tag="VFF70_13335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45723.1"
                     /translation="MASINKAESSDPHDLVTTVVVNWNGRAFLNKCLSSLVAQTYQSH
                     EIILVDNASTDGSADYVEAKWPSIKVLRSRTNIGFAAGNNLAIQRSTGKYVALLNNDA
                     YAEPDWLAKMIEVVDQHQDVGMIACKMLFADRPTIINSTGICIDRVGIAWDRCGGEVE
                     SNDTEPPTEIFGPCAGAALYRRNMLSEIGLFDEDFFMYLEDVDLAWRARRSGWRCLYV
                     PMARVVHYHSASSTEGSPFKSFHLGRNKVWLIIKNYPFAQLWAYAPLLIGYDLAAVIY
                     ALIGRRDIHALRGRLAGWAGARKMWRKRQAASSLRRDDLAWLSPIEPPWLVPHRYRHL
                     SERRNK"
     gene            complement(1537..3093)
                     /locus_tag="VFF70_13340"
     CDS             complement(1537..3093)
                     /locus_tag="VFF70_13340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45724.1"
                     /translation="MRVGIYDRWLYTLGGGERHMLALAQHLSIDHVVEILTHQAVDLN
                     SAGRKLNIDTRGLRLRCVPEMPDPALSLITQQYDLFIQASQASFIPSQARRSLMLVFF
                     PTPIHLSRSARLRRSVGLWLRRQLMQPIYQRGFYSAEYTEGRFVRWTDGHAIVLVPIG
                     SGRVKLSMLIDARGANSKVPVTLRLNDRVIDQLEVSESDRALPVQVDLQAGIGGLAVL
                     EVVSPTRTEWLSPGSSRSIGVAISDLQIQHWRARLYSLLFDRLFKELGLRLLSIPDQV
                     DADTLDSYSILCANSEFTREWIQRYWGRDSQIFHPPVDVAAFQPADKRNIILTVGRIF
                     AGSHNKKHLPMIETFKRMTRSGLSGWEFHIVGSVPPEAVHQDYLKRIRSAAQGFPIYV
                     HTDLPFDQLQELYGAARIYWHASGYGEDEQRDPIKFEHFGITTVEAMAAGAVPVVIGK
                     AGQLEVVEHDRNGLHWQTLDELIGYTQQLITDKSMWQRLSDGARERSRSFGRAAFDRR
                     VKELLVELEV"
     gene            complement(3110..3823)
                     /locus_tag="VFF70_13345"
     CDS             complement(3110..3823)
                     /locus_tag="VFF70_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45725.1"
                     /translation="MKSSLTVQAVLSDIEFEIAQGTISRQELGQGVRAAKQYQNKLRA
                     ETFQTGRALADLHELVDRQFQINDIHLTLLQEMAAAIQSLQLDLQRVGRLRKSTPAMT
                     AQSEASPADWLATPIDLSEYGEIEPAEWWQPEQIEQAMRSDALSLKSEVRAVRAPIIG
                     SLITRVRSALHSLVLFYINLLAQRQTQVNQVYGERILQLSRLHQMQYEQLEMLKIQLA
                     GSAGHAPDTESPVDTQHST"
     gene            complement(3820..>5064)
                     /locus_tag="VFF70_13350"
     CDS             complement(3820..>5064)
                     /locus_tag="VFF70_13350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY45726.1"
                     /translation="SSVYPPAQLEGVGRHTHLMARGLFECGHTVHVITRGDRDQISFY
                     DGAYVHQLAQRHNRYDQYQMLRYLHSILNHSHAVYDCVRQLILNDGIQIVDSPVWQID
                     GLVTAISGVAPVSVRVQTALRQIASIQRDRDDDVRLIGELEQSLIEHAAYLVPNSQAT
                     LGAVRRIYGTFPAVDRYSIIPHGIVPVPDDAVRPFDLQHVPNKFTVLYIGRLEKRKGI
                     PDLFQAIPQILKQVPNVEFIIAGDDNSQYDGFRQQTGVDYPAYFADHFRDVASRVQFL
                     GGVSDSKLQQLYQSCDLFVAPSLYESFGLIYLEAMNYAKPVIGCRAGGIPEVIDDGVT
                     GKLVDPESPIALAEAIVSLLQAPAKLREMGLAGRQRLLDKFTYLQMARGFERVYQSVV
                     QYREVNQSGDGQGSAIDRWSTR"
BASE COUNT         1287 a         1410 c         1254 g         1113 t
ORIGIN      
        1 ctcatgcggc aaaacattgt ggcaaataaa gatcgttggg atgccgtgcc ggcgggccca
       61 acttgccagc gaggccgtca tcggcgccat gaatggcacc caccactgga taacaacccg
      121 atcgggcttc caggtttgcc aatcctttac aacacgccgc cagctaagtg gccaccacgg
      181 aatcagccag cgccgcacct cgatgtcgaa caatggcttc gggcttggat cgatctggct
      241 gcgtccaggg aagagccaat tgggatactg ttgcttgaaa ccatatagcc gcaactcaca
      301 ttgctggctg agatgaaacg ccaacagcga tgtatactgc gcaatgccgc ctcgatgcgg
      361 atatgttgga ccaacaacgg caactttcac gagaatcctt tttccattaa aagcaaacgg
      421 atgataccgt aatcgcgttg aggatcaatg gcggatctgg catcgaatcg atcgcgtgtc
      481 tccaacaagc ttcgggcaga cttaactact tgttacgccg ctctgacaaa tgtcgatagc
      541 gatggggcac caaccagggc ggctcgatcg gcgacagcca cgctaaatca tcacgccgca
      601 acgacgatgc agcctgtcgc ttgcgccaca tttttcttgc gcctgcccac ccggccaatc
      661 gcccgcgcaa ggcgtgaata tcgcgccgcc caatcaaggc atagattacc gccgctaaat
      721 catagccgat tagtaatggt gcgtacgccc acaattgcgc aaacggataa tttttgatta
      781 tgagccacac tttgttgcgt cccaaatgaa aacttttaaa cggtgatcct tccgtactcg
      841 aagccgaatg gtaatgcacg acgcgtgcca tgggcacata gagacaacgc caaccactac
      901 gccgcgctcg ccacgcgaga tcaacatctt caagatacat gaagaaatct tcgtcaaaca
      961 ggccaatttc actcaacatg ttgcggcgat ataaagccgc accagcgcat ggcccaaaga
     1021 tttcagttgg cggttcggtg tcatttgatt caacctcgcc tccacatcga tcccaggcaa
     1081 tgccaacgcg atcgatgcaa attccagttg agttgataat cgtcggccga tcagcaaaca
     1141 acattttgca ggcgatcatg cccacatcct gatgttgatc aacgacctcg atcatcttcg
     1201 ctagccaatc gggttcagca taggcgtcat tgttgagtaa agccacatac ttgccagtcg
     1261 atcgttgaat cgccagattg tttcccgcgg caaatccaat gttggtccgt gaccgcagca
     1321 ctttaatcga aggccacttg gcttcaacgt aatctgcgga gccgtccgtc gaggcattat
     1381 cgactaaaat aatctcatgc gactgatagg tctgcgccac taatgacgac aagcatttat
     1441 taagaaaagc gcggccattc caattgacga caaccgtagt gactagatcg tgcggatcgc
     1501 tcgattccgc tttattgatc gacgccatgc tccgactcac acttctagct caacgagcaa
     1561 ctcttttacc cgacgatcga acgccgcacg cccaaaagat cgactgcgtt cacgagcacc
     1621 gtcgctcaag cgctgccaca tggacttatc agtgatcaat tgttgagtat aaccaatcaa
     1681 ttcatccaac gtctgccaat gcaggccgtt gcgatcgtgt tcaacgactt cgagttgacc
     1741 ggccttgccg ataactaccg gcactgcgcc ggcagccatc gcctcaacag tggtgatacc
     1801 aaagtgttca aacttgatcg gatcacgctg ctcatcctcg ccatagccac ttgcatgcca
     1861 gtaaattcga gccgcgccat acagttcttg cagttggtcg aacggcaaat ccgtatggac
     1921 gtagattgga aagccttgcg cggcggacct aatgcgcttt agataatcct gatgcaccgc
     1981 ctcaggcggc acactgccga caatatgaaa ttcccaacca ctcaaacctg accgcgtcat
     2041 gcgcttgaag gtttcgatca tcggtagatg ttttttgtta tgactgccag caaagatgcg
     2101 cccgacggtt agaataatgt tccgtttatc agccggctga aacgcagcca catcaaccgg
     2161 cggatggaat atttgactat cgcgtcccca ataacgctgt atccattcgc gtgtaaattc
     2221 agaattagcg caaagaatgg aatagctatc gagggtgtcg gcatcgactt gatcaggaat
     2281 gcttaataac cgcaggccca attctttgaa cagacggtcg aacaagagcg aatacaatcg
     2341 agcgcgccaa tgctgaattt gcagatcact gatcgcaact ccaatcgatc ggctgctgcc
     2401 cggacttaac cactcagtcc gtgttgggct gacgacttcg agcacggcca gtccgccgat
     2461 gcccgcttga agatccacct gaacgggcaa agcccgatcg gattctgaaa cttccaattg
     2521 atcgatcacg cgatcgttta ggcgcagggt cacggggact ttcgagttcg cgccgcgggc
     2581 atcgatcaac atgctcagct ttacgcgacc tgatccgatc ggcactagca ctatcgcgtg
     2641 accatccgtc caccgaacga atcgaccctc agtgtattcg gcactgtaaa agccacgttg
     2701 atagatcggc tgcatgagct gacgccgcaa ccacaagccc acggatcttc gaagccgcgc
     2761 agagcgggat agatgaatcg gcgttgggaa gaaaaccaac atcaggctgc ggcgggcctg
     2821 cgagggaatg aaactggctt gtgaggcttg aataaataga tcgtattgct gcgtgataag
     2881 tgaaagtgct ggatcgggca tttccggaac acaccgcaat cgcaagccac gagtgtcgat
     2941 attcagttta cggcccgctg aattcaaatc aactgcttga tgtgtcagaa tctccacaac
     3001 gtgatcgatc gacaagtgct gcgcaagtgc cagcatgtgc cgctcgccgc cgccgagggt
     3061 atacaaccag cgatcgtaaa tgccaactct cacgaaacat tcctggcgat caagtgctat
     3121 gctgggtgtc gactggcgac tcagtatctg gcgcgtgacc agccgatcca gctaactgaa
     3181 tcttgagcat ctccagttgt tcatattgca tttgatgcag ccggctgagc tggaggatac
     3241 gttcgccata gacttgattg acttgggtct gccgctgcgc caatagattg atgtagaaca
     3301 agacgaggct gtgcagcgca ctccgaacgc gagtgatcag cgagccgatg atcggagcac
     3361 gcaccgctcg aacttccgac ttgagggaca gcgcatcgga tcgcatcgcc tgctcaatct
     3421 gttctggctg ccaccactca gctggctcaa tctcgccata ttctgaaaga tcgatcgggg
     3481 tagccagcca atcagctggt gacgcttcag attgagcagt catcgccggc gtggacttcc
     3541 gtaatcgacc aacacgctgt agatccaact gtagtgattg aatggcagcc gccatttctt
     3601 gcagcagagt taaatggata tcattgatct ggaactgccg gtcaaccagc tcatgcaaat
     3661 cggcgagtgc gcgaccggtt tggaatgtct cagcgcgcaa tttattttga tactgcttgg
     3721 ccgcccgcac accctgccct aattcctgac gcgagatggt gccctgcgca atctcaaact
     3781 caatatcact cagcacagct tgtacagtta gcgaactttt catctagtgc tccaacgatc
     3841 gatcgccgag ccttgtccgt cgcccgattg attgacctcg cgatattgaa ctaccgattg
     3901 atacacgcgc tcaaaaccac gcgccatctg aaggtaggtg aatttatcca ataatcgctg
     3961 acggccagcc aagcccattt ctcgcaattt ggcaggagcc tgcagcaacg aaacaatcgc
     4021 ttcagctaat gcgatcggtg attccggatc aaccagctta ccggtcacgc catcatcgat
     4081 cacttcggga atgccacccg cccggcagcc gattaccggt ttggcataat tcattgcctc
     4141 cagataaatc agcccaaacg attcatacag ggatggcgcg acaaacaaat cgcaggattg
     4201 atatagctgt tggagtttag aatcgctgac tccccccaag aactgaacac gcgaagcaac
     4261 atctcgaaaa tggtcggcga agtaggcggg gtaatcgaca cccgtctgct gacgaaagcc
     4321 atcatactgg ctattgtcat caccggcaat aataaattct acattgggaa cctgcttgag
     4381 tatctgcggg atcgcttgaa acaggtcagg gatgcctttt cgcttttcca accgtccgat
     4441 gtagagaacg gtgaacttgt tgggaacatg ctgcaaatcg aatggacgca ccgcgtcgtc
     4501 gggcacgggg acgatcccat gcggaatgat cgaatagcga tcgacagcgg ggaatgtgcc
     4561 atagatccgt cgcacagcgc ccaatgtggc ttgcgaatta ggcacgagat aagcggcgtg
     4621 ttcgatcagg ctttgctcta gctcgccgat caagcgcaca tcatcatccc gatcgcgctg
     4681 gattgaagcg atctggcgca gggctgtctg cacgcgcacg ctaaccggag cgacgccgct
     4741 aatggccgta accaatccat cgatttgcca aactggcgaa tcgacaattt gaattccgtc
     4801 gttcaagatc aactgccgaa cacaatcgta aacagcatgg ctgtgattca atatcgaatg
     4861 cagataccgc agcatctgat attgatcgta gcggttatgg cgttgggcta attgatgaac
     4921 gtaagcgcca tcataaaaac tgatttgatc tctgtcaccg cgagtgataa catggacagt
     4981 gtgaccacat tcaaacagtc cgcgcgccat caaatgagtg tgacgaccaa cgccctcgag
     5041 ttgagccggc ggataaacgc tgct
//
LOCUS       DASRTJ010000659         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_290738,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000659 DASRTJ010000000
VERSION     DASRTJ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290738"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="panB"
                     /locus_tag="VFF70_13355"
     CDS             complement(<1..740)
                     /gene="panB"
                     /locus_tag="VFF70_13355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZY45727.1"
                     /translation="MTDRKKITLPMLQKKMLDGIPITMITCYDYAMAYLVEQAEIDMV
                     LVGDSLGMTMLGYEGTLPVTMDDMMRHTAAVRRGSPTSWLIGDMPYMSYQPSDEIAVK
                     NAGRFMAETGCDSVKLEGGRQMASRVKAIADAGIPVMGHLGLTPQSVSALGGFKVQGK
                     GADQAARIVEDAKILEEAGAFSILFEMIPDRVQKVITERAHIPIISLGSGPDAHGQLL
                     IFHDMFGLYPRFTPKMAKVFGNAGEVIL"
     gene            complement(785..1681)
                     /locus_tag="VFF70_13360"
     CDS             complement(785..1681)
                     /locus_tag="VFF70_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZY45728.1"
                     /translation="MNLTGLHLLLTYQCTFECDHCFVWGSPKQHGTLTLDQIREILKQ
                     AKAYGAIESIYFEGGEPFLYYPVLLQAVREARQFGFQVGIVSNGYWAISVDDAIEWLK
                     PFAGLISDLSISSDLYHYDEAISRQVINARTAADRLGIPIGVISVAQPATTDATSSIG
                     QLPTGESSVMYRGRAADELAPRARLQDWPQFTECPHEDLREPGRVHVDPLGNVHLCQG
                     IAIGNLFQTSLREICETYDPASHSIAGPLLAGGPVELVRRYGLPHADQYADACHLCDA
                     ARRMLRDRFPAILMPDQMYGVF"
     gene            complement(1774..>2292)
                     /locus_tag="VFF70_13365"
     CDS             complement(1774..>2292)
                     /locus_tag="VFF70_13365"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZY45729.1"
                     /translation="PKMFLGAAASPFASEPRFQAIREHKKVNAGAQFFQTNLVYDVDG
                     LEIWLNELAKRNILDKVFILIGITPIKSLKVALRMNKEVPGVTVPDRLLKRLEAAGDR
                     ADEEGVQIALELIDQIKTKQGVHGIHIMAIGWEEIVPRIVREAGLLPPDFIEPPKAPV
                     KEKKPIGEPVGV"
BASE COUNT          504 a          686 c          590 g          512 t
ORIGIN      
        1 ttcaagatca cttcgcccgc attgccgaaa accttcgcca tcttcggcgt gaagcgcgga
       61 tacaggccga acatatcatg gaagatcaac aattgaccgt gcgcgtcagg cccggagccg
      121 agactgatga tcgggatatg ggcgcgctcg gtaatgacct tctgcacgcg atcggggatc
      181 atctcgaata agatcgagaa cgcgcccgcc tcctccagaa tcttcgcatc ctcgacgatg
      241 cgtgccgctt gatcggcccc tttgccttgc accttgaatc cgcccaacgc cgaaacactc
      301 tgcggtgtga ggccgaggtg gcccatcacc ggaatgcccg catcggcgat cgctttgacg
      361 cgcgaagcca tctgccgccc gccttcgagc ttcactgaat cgcagccggt ctcggccatg
      421 aagcggcctg cattctttac ggcaatctca tctgatggtt gataactcat atacggcatg
      481 tcgccgatca accagctcgt tggcgaaccg cgccgcacgg cggccgtgtg ccgcatcata
      541 tcgtccatcg ttacgggcag tgtgccttcg tagccgagca tcgtcatgcc cagactgtcg
      601 cccaccaaca ccatatcgat ctccgcctgc tcgacgaggt acgccatcgc gtaatcgtag
      661 caagtgatca tcgtgatggg gatgccatct aacattttct tttgcaacat cggcagtgta
      721 attttctttc ggtcagtcat agcagactcc tttcaagtcg aaattgaatt gtgacagtca
      781 agattcagaa tactccatac atttgatcgg gcatcaagat tgccgggaat cgatcgcgca
      841 gcattcgacg cgcggcatca cacagatggc acgcatcggc atattgatcg gcatgtggca
      901 atccgtagcg acgcaccaac tcgacggggc cgccagcgag cagcggaccc gcgatcgaat
      961 gtgaggctgg atcgtatgtt tcacaaattt cacgcaatga cgtttggaaa aggttaccga
     1021 tcgcaatgcc ctggcacaga tgaacattgc ccagcggatc gacgtgcacg cggcccggct
     1081 cgcgcagatc ttcgtgtgga cattccgtga actgcggcca atcttgaaga cgcgcacgcg
     1141 gggccagctc gtctgcggca cgtccgcgat acatcacgct cgattcaccg gtgggcagtt
     1201 gcccgatcga cgatgtcgcg tctgtcgtcg caggctgcgc aacgctgatg actccgatcg
     1261 ggatgcccaa tcgatcggct gccgtacgcg cgttgatcac ttgtcggcta atcgcttcgt
     1321 cgtagtgata cagatcgctg ctgatcgata gatcgctgat caagccggcg aaaggtttca
     1381 gccattcgat cgcatcgtca acgctgatcg cccaatagcc gttggatacg atgccaactt
     1441 gaaagccgaa ctgtcgcgct tcgcgtaccg cctgcaacaa aaccgggtaa tacagaaacg
     1501 gttcaccgcc ttcgaaatag atcgattcga tcgcgccata ggctttagct tgcttgagaa
     1561 tttcacgtat ctgatccaac gttaaagtgc catgctgctt cgggctgccc cacacgaagc
     1621 agtgatcgca ttcgaacgtg cattgataag tcaagagtaa atgtagacca gtgaggttca
     1681 tacaagtaac ctccctgcat caatacgcaa cggatcatcg ctggagttaa cgcacttgat
     1741 gatccgctgc tgcacttcga tcattatcgc ctgctatacg ccgactggtt caccgatcgg
     1801 tttcttctct ttcacaggcg ctttcggcgg ctcgataaaa tccggcggca gcaagccggc
     1861 ttctctgacg atacgcggca caatctcttc ccagccgatc gccataatgt gaatcccgtg
     1921 aacgccctgc ttcgttttga tctgatcgat caactccagc gcgatctgca cgccttcctc
     1981 atcagcccga tcgcccgcgg cctctaagcg cttgagtaac cgatcgggca cggtgacgcc
     2041 cggcacttct ttgttcatcc gcagcgcgac cttcaaactt ttgatcggcg tgatcccgat
     2101 caagatgaag accttatcga gaatgttgcg cttcgccaat tcattcaacc aaatctccag
     2161 cccgtcaaca tcgtagacga ggtttgtttg gaagaactgc gcgcccgcgt tgactttctt
     2221 gtgctcgcgg atcgcctgaa aacgcggctc ggatgcaaag ggcgaagcgg ccgcacccaa
     2281 aaacattttg gg
//
LOCUS       DASRTJ010000660         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_290902,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000660 DASRTJ010000000
VERSION     DASRTJ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290902"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFF70_13370"
     CDS             complement(<1..253)
                     /locus_tag="VFF70_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45730.1"
                     /translation="MMDAVQLLLLAVLLFGVGAVASLLLNKLSRAARVTAGLIGAIAS
                     AVGLVAVVQAAIGPSAVLEIPAPVPFGHFNLQMDGLSTLM"
     gene            complement(284..895)
                     /locus_tag="VFF70_13375"
     CDS             complement(284..895)
                     /locus_tag="VFF70_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZY45731.1"
                     /translation="MNQFVIADPKLCIGCYACVAGCVESHRQVGLQAYPRLYIIHTPP
                     VGTMPIQCRQCEEPACAAVCPVKAISYQHNSIYLNESLCIGCKMCAVACPFGAIIPGG
                     TPTPKFDFNVGQYTYLNTPYQADPMYLREFALQERLSLLNWNIGQKTVAVKCDLCYFR
                     EEGPACVSACPHKALSLVDEQTDRPQKFIEQTKTVAKLEAGGG"
     gene            complement(1418..1488)
                     /locus_tag="VFF70_13380"
     tRNA            complement(1418..1488)
                     /locus_tag="VFF70_13380"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1454..1456),aa:Cys,seq:gca)
     gene            complement(1542..1613)
                     /locus_tag="VFF70_13385"
     tRNA            complement(1542..1613)
                     /locus_tag="VFF70_13385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Gly,seq:gcc)
     gene            complement(1646..1718)
                     /locus_tag="VFF70_13390"
     tRNA            complement(1646..1718)
                     /locus_tag="VFF70_13390"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1683..1685),aa:Phe,seq:gaa)
     gene            1834..1932
                     /locus_tag="VFF70_13395"
     tRNA            1834..1932
                     /locus_tag="VFF70_13395"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Sec,seq:tca)
     gene            complement(2008..>2442)
                     /locus_tag="VFF70_13400"
     CDS             complement(2008..>2442)
                     /locus_tag="VFF70_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZY45732.1"
                     /translation="HGATLRRYTSGEVLVRQGDAGESLFVIKDGQVKVNVRSESGQST
                     TVATHGPGNFFGEMSLLTGEPRTASVVAQIDTEVVVVDKADVTKLLMADSSIAEALSN
                     VIESRLRGTAEQIALEEEARSGKLTAQQSPLLNRIRSFFGIG"
BASE COUNT          598 a          654 c          626 g          564 t
ORIGIN      
        1 ccattaatgt cgagaggcca tccatctgca agttgaaatg accgaaggga actggcgcag
       61 gaatttctaa aacggctgaa gggccgatcg cagcttgaac aaccgccacc agcccgacag
      121 cggaggcgat cgctccaatc aatccggctg taacgcgggc tgcccgactt aacttgttga
      181 gcagcaatga ggcgactgca cccactccaa acaacagcac ggccagtagt agcagctgaa
      241 ctgcatccat catattcctc tacatcgtac gatctttgaa agcttaacct ccccccgctt
      301 ctaatttagc gactgtcttt gtctgctcga taaatttctg tggccgatcg gtttgttcgt
      361 ctaccaggct gagagctttg tgtgggcagg cgctgacgca ggccggccct tcctcgcgga
      421 agtaacacag atcacatttg acagcgactg ttttttgacc gatgttccag ttcaaaagag
      481 acagacgctc ctgcaacgcg aattcacgca gatacatcgg atcagcctga taaggtgtat
      541 tgaggtaggt atattgcccg acattgaaat cgaattttgg ggtcggtgtg ccgccgggga
      601 tgatcgcccc aaagggacag gccaccgcgc acatcttaca accgatgcac aagctttcat
      661 tcaagtaaat ggagttgtgt tgatacgaga tggccttgac cggacagacc gccgcacagg
      721 ccggttcttc acactgacgg cactggatgg gcatcgttcc gacgggcgga gtatggatga
      781 tgtataaacg gggataggcc tgcaagccga cttggcgatg gctctcgacg cagcccgcca
      841 cacaggcata acatccaata cacagttttg gatcagcaat cacaaattgg ttcatgctga
      901 atccttagta gccgttgaaa cgaaggttgt ggttgctgac gaaatggttg aggttcaatc
      961 tcaacggcca acaactgatg acactcaacc gagaaggtaa tgcgagcgat gtcgcattga
     1021 ccgcacatcg acataaccat tttcaggcct gccgtttggt agggtgatcg ttaatagacg
     1081 attgtaataa ttctcacaat agtataaaag agaaccggcc agaagacacc tgtcatttgt
     1141 atgctttcga gtattacatc gatacgggag cgaatatttt ccgaccaaaa aaagtttact
     1201 gaagattacg aatagatgat tagactgaca ccgatcgatt taatatcaat ttcagataga
     1261 tcgaatcagc agggcaaatt ggtcgatcca ttcaacacga tcgctgaccg atcccgacaa
     1321 agttgataga tatttcgaaa tgatcaagta aggttttatc ttgaggttca gcccaaaaca
     1381 aaacgcccga tcgaaaatga tcggacgttt gagtcagagg cgacggtggg aattgaaccc
     1441 acggtgaggg ttttgcagac ccttgccttg ccacttggcg acgtcgccat tgaaacaaac
     1501 ggctgatgct gcacagcaaa gtcgcatcag ccattggtca gagcgggaga cgggattcgg
     1561 acccgcgatc ttctccttgg caaggagacg ctttaccact aagccactcc cgcgtatgtg
     1621 ccgctcgctc gcccggcaca cagtgtgccg aggcgcagga tcgaactgcg gacaccgcga
     1681 ttttcagtcg cgtgctctac catctgagct acctcggcgg tagcgacgtt attgtaccgt
     1741 acaggcaaat gtctgtcaag caaatttatc agcgcagacc caaaaggttt caaaaacctt
     1801 ccgcgtcttg ttgtaaagtg gtatcatgca cgcggggatg gttggagccc ggtggctccc
     1861 acagtcttca aaattgttgg ctggtcgcgc tgagcgagcg gcggtgggtt cgactcccat
     1921 ccttccccgc cattcacgtc gatgtgccac gcacttttga tcgatcactg ctgattcgat
     1981 ccaactgaag tgcgtggcac tattcgatca accaatcccg aagaacgatc gaattcgatt
     2041 gagcagcggc gattgctggg cggtcagctt gcccgatcgg gcttcctctt ccagtgcgat
     2101 ttgttccgcc gtgccgcgca ggcgcgactc aatcacattc gataacgctt cggcgatgct
     2161 gctatcggcc atcagcagct tggtcacatc ggctttatcg acgaccacga cttccgtatc
     2221 gatctgcgcc acaaccgagg cggtacgtgg ttcaccggtc agcagactca tctcgccgaa
     2281 gaaattgccc ggtccgtggg tggccaccgt cgtcgattgg ccgctctcgc ttctgacatt
     2341 cactttcact tgaccgtctt tgatcacaaa gagtgattca cccgcatcgc cttgtcgtac
     2401 caacacttcg ccgctggtgt aacgccgcag cgtagcacca tg
//
LOCUS       DASRTJ010000661         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_365390,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000661 DASRTJ010000000
VERSION     DASRTJ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365390"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFF70_13405"
     CDS             complement(<1..208)
                     /locus_tag="VFF70_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45733.1"
                     /translation="MVSSDLLEILRCPHCVSGETRHAGEDPGRLQLVNNAWLVCLETD
                     CQRKYPIRDDIPVMLIEEGDKYKNT"
     gene            408..495
                     /locus_tag="VFF70_13410"
     tRNA            408..495
                     /locus_tag="VFF70_13410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:442..444,aa:Ser,seq:tga)
     gene            552..645
                     /locus_tag="VFF70_13415"
     tRNA            552..645
                     /locus_tag="VFF70_13415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:586..588,aa:Ser,seq:gct)
     gene            676..747
                     /locus_tag="VFF70_13420"
     tRNA            676..747
                     /locus_tag="VFF70_13420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:709..711,aa:Arg,seq:gcg)
     gene            831..1538
                     /locus_tag="VFF70_13425"
     CDS             831..1538
                     /locus_tag="VFF70_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45734.1"
                     /translation="MPRILAILIGISLLAGCNTNTTPASAPTVFIPTTVPQSQATSAP
                     VTNAAGTILFQDDFSNKNGDWDQQSSTDGGSNYGSGFYSIQVETADYSIWANPNAQPA
                     FSDVAIDVDAAIANGPEDAGFGLICRYVDTDNFMHAAITADGYYGIVLVKAKQATVLT
                     GQGNLVKSTAINAGKANNHIQFVCKGNEYTLFVNGQKLESAHDSSFIQGAVGLIATTF
                     ATGGVEVRFNKYLVTTP"
     gene            1656..2465
                     /locus_tag="VFF70_13430"
     CDS             1656..2465
                     /locus_tag="VFF70_13430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY45735.1"
                     /translation="MNLQDRVAIITGASSGIGAATAQRLASAGLHVVLAARRTDRLVE
                     VRESILAKGGFALEVETDVTQRESIDRLVDRVMSEYGKIDVLINCAGFGSVGWLDEID
                     PADIRRQFAVNVFGTIEMTQAVLPIMFQQRHGHIINVSSLASKIATPTYSIYASTKFA
                     IDGFSQALRREVRPWGIHVSILYPSSVETEFAEVADIRRKTKVHMPHRFVLSADDVAR
                     KILSRVKHPRSSATLPLIMHGARFFNHFTPWLVDWLTARWVQIERSDKLKI"
     gene            complement(2530..3567)
                     /locus_tag="VFF70_13435"
     CDS             complement(2530..3567)
                     /locus_tag="VFF70_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZY45736.1"
                     /translation="MEEKISISRRQFIRGASVIGAGLGLAAACSPAAPVVGPTPTMDH
                     SADNSQPQQATPDDMDAMHKKGVDTFVAGAGKDDTFWRKPLAFKLDGDTKVFDLTVTA
                     GKWNTTPDQAVDAMMYNSMAPGPEIRVTEGDKIRVVCNNNMTAQSTSIHFHGLLIDNK
                     MDGVPYITQDPIKPGTSFTYEFTVKNPGTHMYHSHHNAAEQVTKGLMGAFIIEPKDKS
                     KEPLVDAEYSLLLNDTGIGLTINGKSFPYTQPIIAKLGQTIRIRYMNEGLLIHPFHLH
                     GLPQQVVAKDGWYLPAPYMCDTLNIAPGERYDVIVKCDAPGVWAYHCHILTHAESAHG
                     MFGMVTAVIVQ"
     gene            3859..4215
                     /locus_tag="VFF70_13440"
     CDS             3859..4215
                     /locus_tag="VFF70_13440"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HZY45737.1"
                     /translation="MKLIYHICPVDYYKSIPANQAYLPLEFDRDGFIHCTRGAEQVVV
                     VANRYYRDDVRPLLLLVIDEERLTAAIKNELAEDSLAYPHLYGPLNRDAIIGVVRMPR
                     LPDGSFQFPDRDQTRS"
BASE COUNT         1047 a         1130 c         1245 g         1119 t
ORIGIN      
        1 gtgtattttt gtacttgtcg ccttcttcga tcaacatgac cggaatatcg tcacgaatgg
       61 gatacttacg ctggcagtcc gtctctagac agaccagcca agcgttattg actaattgca
      121 ggcggcccgg atcttcaccg gcatgccgag tttcaccact gacgcaatgc gggcatcgca
      181 aaatttccaa tagatctgag ctgaccattt tgactctccg tgatcttttt ctgaagtgta
      241 taacgattgc attctaccat attcacaatg cctgagcaaa gatcacgcaa aaatttggcg
      301 agtgtattga cagtgctaga tcggcgtggt atactcgccg acaagttgcg atgcgctaaa
      361 ccttgcgttg tttcgccgca aaattcaggc agggtcaggc tgaatgtgga gggatggccg
      421 agtggtttaa ggcgacggtc ttgaaaaccg ttgtagctct ctgggttacc gggggttcga
      481 atccctctcc ctccgcctta aaaattgatc gctcagctga catctgatta gatgtcacgc
      541 gagtccaaaa aggggaggtg ccggagtggc cgatcggagc cgcctgctaa gcggttgtag
      601 ggctttaaaa ccctacccag ggttcgaatc cctgcctccc cgccagaatt ttaacaacgc
      661 gatgatgtcc gttacacccc tgtagctcag cggatagagc gcggccctgc ggaggctgag
      721 gcttgggttc aattcccaac aggggtatat tttttcctcg tcgatcgatt ccctattaca
      781 aaccactctt cctttctgta ttagtaagat ccgctcaggc ggggatactt atgccacgaa
      841 ttttagctat cttaatcgga ataagtttat tagcagggtg caacactaac acaacgccag
      901 catcagcacc caccgtcttt ataccgacaa ctgtccccca atctcaagcg accagcgcgc
      961 cagtgacgaa tgcggcaggc acgattctgt ttcaagatga tttctcaaac aagaacggcg
     1021 attgggatca gcagagcagc actgacggag gatcgaatta tggcagcggc ttctacagta
     1081 ttcaggtgga gactgcagac tattcgatct gggcgaatcc gaatgcacag ccagcgttta
     1141 gtgacgtggc gattgatgtc gacgcggcga ttgcgaacgg tccggaagac gccggttttg
     1201 gcctaatttg ccggtatgtt gatacggaca actttatgca tgccgcaatt accgccgatg
     1261 gatattatgg tatcgtactg gtgaaggcta agcaggctac cgtgttaacc ggccaaggca
     1321 atttggtgaa gagcactgcc attaacgctg gcaaagctaa caatcatatt caattcgtgt
     1381 gcaaaggcaa tgaatacaca ctgtttgtca acggacaaaa attggagtca gctcatgata
     1441 gcagtttcat tcagggcgct gttggcttga ttgcgactac ttttgcgacg ggcggcgttg
     1501 aggttcgctt caacaagtat ttggtaacaa ctccgtagct gctggttcgt gctgagggag
     1561 gaggcgactc gtaggagagt cgcctctttt tgttagactt gggattttcg tttatcattc
     1621 tgagcgcgtc aaataatctt ttaacgaggc agcacatgaa tcttcaagat cgagtagcca
     1681 ttatcaccgg tgcgtcaagc gggatcggcg cggcgacggc gcaacgtcta gcgagtgcgg
     1741 ggctgcatgt tgtgctggcc gcccgccgca ccgatcgatt ggtcgaagtg cgtgagagta
     1801 ttctggccaa aggtggattc gcgctggaag ttgagactga tgtaacacag cgcgagtcga
     1861 tcgatcgcct ggtcgatcgc gtgatgagcg agtacggcaa gatcgatgtg ctgatcaatt
     1921 gcgcgggatt tggctccgta ggttggctgg acgagatcga tccggcggac atccgtcggc
     1981 agtttgcggt gaatgtgttc ggcacgatcg agatgacaca ggctgtgctg ccgatcatgt
     2041 ttcagcagcg ccacgggcat ataatcaatg tgtcatcgct cgcgagtaag atcgcgacgc
     2101 cgacttacag tatttacgcg tcgacgaaat ttgcgataga tggtttctcg caagcactgc
     2161 ggcgtgaagt tcgaccgtgg ggcattcatg tgtcgatcct ttatccgagc tcggtcgaga
     2221 cggagtttgc tgaagtggcc gatattcggc gcaaaacaaa ggtgcatatg ccgcaccggt
     2281 tcgtgttgag tgcagatgat gttgcgcgta aaattctgag cagggtgaag cacccgcgat
     2341 caagcgccac gctgccgctg atcatgcatg gcgcgcggtt cttcaatcat ttcacacctt
     2401 ggctggttga ttggctcact gcgcgttggg ttcagatcga acgatcggat aaactgaaga
     2461 tctaaaaata gaacgcaggc tccatatcga aacccgcgtt ttcgatcgat cttgaaagtg
     2521 ttgctggatt tattgaacga tgactgccgt caccatgccg aacatgccgt gcgccgattc
     2581 ggcgtgcgtc agaatgtggc agtgatacgc ccacacgccc ggggcatcgc acttgacaat
     2641 gacatcgtag cgttcgccgg gcgcaatgtt caaggtgtcg cacatatacg gcgccggcaa
     2701 ataccagcca tctttggcaa cgacttgctg cggcaagccg tgcaaatgga acgggtgaat
     2761 gagcaggcct tcattcatat agcggatgcg aatggtctgc cccagcttcg cgatgattgg
     2821 ttgagtgtag gggaagcttt tgccattgat agtcaaaccg atgcccgtgt cattgagcag
     2881 cagagaatat tcagcatcga ccagcggttc tttactcttg tctttcggct cgatgatgaa
     2941 tgcacccatc aagcccttgg tgacttgctc ggccgcattg tgatgtgagt gatacatatg
     3001 tgtgccggga ttcttgaccg taaactcgta agtaaagctg gtaccgggct tgatcggatc
     3061 ttgggtgatg tacggtacac catccatctt gttgtcgatg agcaggccat gaaagtggat
     3121 tgacgtgctc tgcgcggtca tgttgttgtt gcaaaccacg cggatcttat cgccttccgt
     3181 tacgcgaatc tccgggccgg gggccatgct gttgtacatc atcgcatcga cagcctgatc
     3241 gggtgtggtg ttccacttgc cggcggtgac ggtaagatcg aagactttgg tatcgccgtc
     3301 cagtttgaaa gccaatggct tgcgccaaaa cgtatcatcc ttgcctgcgc ccgcgacaaa
     3361 tgtatcgaca ccctttttgt gcatcgcatc catgtcatca ggcgtggctt gctgtggctg
     3421 cgagttgtct gcactgtgat ccatcgttgg ggtagggcca acgaccggag ccgcgggtga
     3481 gcaggctgcg gcgagaccca gccccgcgcc gatcaccgat gcgccgcgaa taaattgtcg
     3541 acgcgaaatc gaaatcttct cttccattgt tgatatcctc catcagcgaa tttataagcc
     3601 aggtagtagc ttaccaaaag ccagactgcg agcgagtgct atttgtcccg attttttcag
     3661 ggtcgaatgt catgggttgc gaccaacaaa cgcgatcatt agtctttgag atcgtacgcg
     3721 cggctggcgg cgtttattcc gtgcgtccga ttgagcgttg cagcccgagc ggcaaaacct
     3781 gatccaaagc gctgccattt gacgcgcaaa tcaaaacaca gtagacttac agcacactcg
     3841 actctgaggc gaagtcccat gaaattgatc tatcatatct gccccgtcga ttactacaaa
     3901 tcgattccgg cgaaccaagc ttatctgccc cttgaatttg atcgagatgg cttcatccac
     3961 tgcacgcgcg gcgcagagca agtggtcgtc gtcgccaatc gatattaccg cgacgacgtg
     4021 cggccgctgt tattgctggt gatcgatgaa gaacgcctaa cggccgcgat taaaaatgaa
     4081 ctcgccgaag atagcctggc ctatccacac ctatatggcc cgctcaatcg cgacgcaatc
     4141 attggtgttg tacgtatgcc gcgtctgccc gatggctcct ttcaattccc cgatcgcgat
     4201 caaacgcgca gctaacaacg aagataatgg tcagaaaaat ttaagataga ctcaggactt
     4261 tagatcattg cgcctaagga gggcttgtgc tatggtaata gtacctctcg atcagatgcc
     4321 cggttcatcc tcgaaggcgg gcatcaaaat cttgattctt gcatggaagg aggccggtat
     4381 ttgacggata gcactgagcc accggaaaac gcagagccga tgttcgtgca gcaggtgtgt
     4441 tgcgagttta ttaagaacct gactgtgaac cgttcgggtt ccggctgatg attgcataac
     4501 cgctcgttac tttgaagcgc tcgtatggca ttgcaacagg g
//
LOCUS       DASRTJ010000662         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_291295,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000662 DASRTJ010000000
VERSION     DASRTJ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291295"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFF70_13445"
     CDS             <1..505
                     /locus_tag="VFF70_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45738.1"
                     /translation="QLDRFLGKNDEAIQEYQNALKWNPDCADCWGGLSDIFSSAGNYT
                     AVETITLAFMQTHADFVPGLRTLARNVYLPSGRLSEAIDTQSQVVSLAIQDPNVWDDY
                     RVLAVMLAQAGRFPEALQAAQTGLTKAPQDKQTEMQNLITQLQQQLGVGPTLTNTVPG
                     LNPTPTP"
     gene            557..1609
                     /locus_tag="VFF70_13450"
     CDS             557..1609
                     /locus_tag="VFF70_13450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZY45739.1"
                     /translation="MTTAMLVFAGALLIAFSATPLARRLAPRLGVMDQPNPRKVHAQP
                     MPLLGGVAIVIASIVALLLFQDHFEIQQLIGTFFGAALVSLLGIVDDRWGLRPLLKLL
                     GQIFAAVILIVSGLQVQSFPFEWLNIFVTLVWIVGITNAFNLLDNMDGLSGGVAAVAA
                     AFFTLMAALTSQTYVGALAAALLGAAIGFLVYNFNPASIFMGDTGSLFLGFMLSAIGI
                     KLRFPSNVTFVTWMVPVLVLGLPIFDTTLVFISRLRRGQNPLTTPGKDHVSHRLVALG
                     FTTREAVMIHYLAGVAFGMIAIFVTQASVLEGYIAGGATALAALYFLLRLEFKRNAKV
                     ASSNATSHSTSIDEPH"
     gene            1619..2203
                     /locus_tag="VFF70_13455"
     CDS             1619..2203
                     /locus_tag="VFF70_13455"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZY45740.1"
                     /translation="MSTETTPNTATKPKSRALQLITLLLAVGISLAIIVITNQYREQI
                     QSLGNAGYLGLFVISIIGNATLFFPAPVFIVACAAGLALGPIPVGIVAGAGAAIGELT
                     GYLAGYGGEAILPEGKLYQRLHAFMDRHGMLAIFLLAAVPNPIFDVGGLIAGVLKMPV
                     WKFLLAAIIGKSIRLGFTAWACLSGIPFLQQIFK"
     gene            2749..>4757
                     /locus_tag="VFF70_13460"
     CDS             2749..>4757
                     /locus_tag="VFF70_13460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZY45741.1"
                     /translation="MNETSMPKTYDFTSTEQRLYEWWWQHGWFKPEAARDQSAEPFVI
                     SIPPPNVTGELHWGHAMFVSIEDLMIRYARMRGQRALWVPGMDHAGIATQLQVEKHLL
                     RTEEVTKDELGREEFVKRVWEWKTKYGGIIQNQIRRLGASADWDRERFTLDAGLSRAV
                     REAFVRLYDKGLIYRGTRLVNWSPGLKTAVSDIEVEYSEEQVTLYFFKYRLADSDEYI
                     PVATIRPETILGDTAVAVNPIDDRYKHLIGRTVKVPILDREIPVIGDKAVSLEFGTGA
                     LKVTPGHDPIDYDIAQRHNLPIINILTIEAKINENGGAYQGLDRDVARKKFWADMEQA
                     GLTLKTEPYTTSIPRAQRGGEVIEPMMSTQWYVKIKPLADPAIEAVRSGAIKIVPERF
                     EKVYFNWMENIKDWCISRQLWWGHRIPVWTCGHGHEFAARIDPTACPQCEDKNLQQDP
                     DVLDTWFSSGLWPFSTLGWPDETIDLKTFYPTSVLETGYDILFFWVARMIMFGLEMTG
                     QPPFHTVYLHGLVRDEHGRKMSKSLGNAIDPVQLLDEFGTDAFRFTLITAGTPGNDLN
                     LGVNRIESNRNFANKIWNVARFVISNWENVDPKVEWGDSVANYQLPDKWILSRLNTTI
                     TSVTRLINEYQYGEAGRTAYDFLWNEYADWYIEISKIALNSTDEN"
BASE COUNT         1117 a         1302 c         1253 g         1085 t
ORIGIN      
        1 gcagctcgat cgcttcttgg gcaagaatga tgaggcaatc caagaatatc aaaacgcgct
       61 gaagtggaat ccggactgtg ccgattgctg gggcggcctg tccgatatat tcagctcggc
      121 gggcaactat accgccgtcg agacgattac tttagccttc atgcaaacgc acgccgattt
      181 tgtgcctggc ctgcgcacac tggcccgcaa tgtttacctc ccttccggtc gattgagcga
      241 agcgatcgac acgcaatcgc aagtggtgtc attagccatt caggatccaa atgtctggga
      301 cgattatcga gtgttggcgg tcatgttggc gcaagccggc cgcttcccgg aagcgctgca
      361 agccgctcaa accggcttaa ccaaagcgcc gcaggacaag caaactgaaa tgcagaatct
      421 aatcacgcag ctgcaacaac agctgggcgt tgggccgaca ctcacgaaca cagtccctgg
      481 acttaacccg acgccaacac cataacttca gcatcgagag acgttccagt cgaacgtctc
      541 actgagatca cattctatga cgactgctat gttggtcttc gccggcgcgc tgctgattgc
      601 tttcagtgca acgcccctcg cccgcaggct tgcgccgcgc ctaggcgtca tggatcagcc
      661 caatccgcgc aaagtccatg ctcaaccaat gcccttgctc ggcggtgtgg caatcgtaat
      721 cgccagcatc gtcgctttac tgttgttcca ggatcacttt gagatacaac aactcatcgg
      781 caccttcttc ggtgcggcgc tagtctctct gttgggcatc gtggacgatc gctggggatt
      841 gcgtccgctg ctaaaattgt tggggcaaat tttcgccgca gtgattttga tcgtctccgg
      901 cttgcaggtt caatcttttc cattcgaatg gctaaacatc tttgtgacgc tcgtgtggat
      961 cgtcggtatc acgaatgcct tcaacctgct tgataacatg gatggactat cgggcggggt
     1021 agccgcggtc gcagcggcat tcttcacgtt gatggccgcc ttaaccagtc aaacttacgt
     1081 gggcgcgtta gcggccgctt tgctgggagc agcgatcgga ttcctggttt acaacttcaa
     1141 tccggccagc atcttcatgg gcgataccgg cagtttgttc ctcggtttca tgttatccgc
     1201 catcggcatc aaactgcgct tcccatcgaa cgtcaccttt gtcacgtgga tggtgcctgt
     1261 cttggtcttg ggcctgccga tcttcgacac gacgctggtc ttcatttcgc gattgcggcg
     1321 cggccagaat ccgctgacca cgcccggtaa agatcatgtg tcccaccgat tggtggcgct
     1381 gggcttcacg acccgcgaag cggtgatgat ccactatctc gccggcgtag cattcggcat
     1441 gatcgcgatc ttcgtgacac aagccagtgt gcttgaaggc tatatcgccg gcggcgcaac
     1501 cgcactggcc gcactgtatt ttctgcttcg actggaattc aagcgcaacg ccaaagtggc
     1561 gagttcaaac gctacctcac actcaacctc gatcgacgag ccgcactaag cacagcccat
     1621 gtcaactgag accacgccta acactgccac gaaaccgaaa tcgcgcgccc tacaactgat
     1681 cacactgttg ttggccgtcg gcatcagtct ggcgatcatc gtgatcacca atcaatatcg
     1741 cgaacagatt caaagtttag gcaacgccgg ttatctcggt ttgtttgtga tcagcatcat
     1801 cggtaatgcg actttgtttt ttcccgcgcc agtcttcatc gttgcctgcg ccgctggact
     1861 ggcactcggt ccaattccag ttggtatcgt cgccggagcg ggtgcggcga tcggcgaact
     1921 gaccggttat ctggcgggtt acggcggcga ggcgatcctg cccgaaggca aactgtatca
     1981 acgcttgcac gcgttcatgg atcgacacgg catgttagca atatttctgc tggccgctgt
     2041 ccccaatccg atcttcgatg tgggtggcct gatcgccgga gtgttgaaga tgccggtctg
     2101 gaaattttta ctggccgcca tcatcggcaa atcgatccgc ctgggcttca cggcctgggc
     2161 gtgtctcagc ggaattccat tcttgcagca aatcttcaaa taataatcgg acgcggcttg
     2221 cgcagacgct tacatcgcac tgcggcgcat gcagtgcagg tgttaacact gattttattt
     2281 ttatctagga aatccattaa atctgcgccc aaaattttgc attgacaact ctttcaactc
     2341 ggctataatc agcgcaatac agtgaacgac gttgaacgag aggagtaggc gtttgacgac
     2401 cgacagaaat taagggtcac tggctgaaag cccttatcgg ttcagcagcg ccgaagtcgc
     2461 tcgggagttg attcgctcaa cagagccaaa tgaccacaaa gactcaaagt cacaaagaaa
     2521 tattttacct tagtgtttta gcgtcttagt ggtaaaccag ctcttcatag gcgagtccgg
     2581 gttagcccgt tacagccgat cgagtgtctt ttgagcaatt ttgtaaattg cttaacagaa
     2641 accaaggtgg taccacggaa gcctctttcg tccttgcaaa cgagagaggc ttttattttt
     2701 catttgtcgt ttgctcattt gtcattttga gcatgaggag atcagactat gaatgaaaca
     2761 tccatgccga agacttacga cttcacctcg accgaacagc gtctatacga atggtggtgg
     2821 cagcacggtt ggttcaaacc cgaagcagcg cgcgatcaat cggctgagcc gttcgtgatc
     2881 tcgatcccgc cgccaaatgt caccggcgaa ctgcattggg gccacgccat gttcgtcagc
     2941 atcgaagacc tgatgatccg ctatgcacgc atgcgaggcc agcgcgcctt gtgggtgccc
     3001 ggtatggatc acgctggcat cgcgacacaa ttgcaggttg aaaaacattt gctgcgcacc
     3061 gaagaagtca ccaaagacga actcggccgc gaagaatttg tgaagcgcgt gtgggaatgg
     3121 aaaaccaaat acggcggcat cattcaaaat cagattcgcc gcctgggcgc atcggccgat
     3181 tgggatcgcg aacgctttac gctcgatgcc ggactcagcc gcgccgtgcg cgaagccttc
     3241 gtccgattgt acgacaaagg cttaatctat cgcggcaccc gattggtcaa ctggtctccc
     3301 ggcttgaaga ccgccgtgtc agatatcgaa gtggaataca gtgaagagca agtgaccttg
     3361 tacttcttca aataccgatt ggctgatagc gacgaatata ttccggtagc aaccatccga
     3421 cctgaaacga ttttgggcga tacagcggta gccgtgaacc cgatcgacga tcgatacaag
     3481 cacctgatcg gacgaaccgt gaaagtgccg atcctcgatc gggaaattcc ggtgatcggc
     3541 gataaagccg tgtcgctcga attcggcacg ggcgcactca aagtcacacc ggggcatgat
     3601 ccgatcgatt acgacattgc tcaacgccac aacttgccga tcatcaacat cctgacgatc
     3661 gaagcgaaga tcaatgaaaa cggcggtgcc tatcaaggcc tcgatcgaga tgtggcgcgc
     3721 aaaaaattct gggctgacat ggaacaagcc ggactcacgc tcaagactga accgtacaca
     3781 accagcatcc cacgcgccca acgcggcggc gaagtaatcg aaccgatgat gtccacgcaa
     3841 tggtacgtaa agatcaagcc gctggccgat ccggcgatcg aagcggtgcg atcgggcgcg
     3901 atcaagattg tgccggagcg ctttgaaaaa gtttacttca actggatgga gaatatcaaa
     3961 gactggtgca tcagccggca gttgtggtgg gggcatcgca ttccggtgtg gacgtgcggc
     4021 cacggtcatg aattcgccgc acgcatcgat ccgactgcct gtccacagtg cgaagataaa
     4081 aatcttcagc aggatccgga cgtgctcgac acgtggttct cttcgggcct gtggccgttc
     4141 tcgactctcg gttggccgga tgaaacgatc gatctcaaaa cattctatcc gaccagcgtg
     4201 ctcgaaaccg gctacgacat tttattcttc tgggtcgcgc gcatgatcat gttcggtttg
     4261 gaaatgacgg gccagccgcc atttcatacc gtatacttgc acggccttgt gcgcgatgaa
     4321 catggccgca agatgagcaa gtccctcggc aacgcgatcg atccagtgca attactcgat
     4381 gaattcggca cggatgcctt tcgcttcacg ttgatcacgg cgggcacgcc cggcaacgat
     4441 ctcaatctcg gcgttaaccg catcgaatca aatcgcaact tcgccaacaa aatttggaac
     4501 gtggcgcgct tcgtgatttc gaattgggaa aatgtggacc caaaagtgga atggggagac
     4561 tcggttgcta attatcaact accagacaaa tggattttgt cacgcctgaa cacgacgatc
     4621 actagcgtca cgcgcctgat caatgaatat caatacggcg aagcaggaag aaccgcatac
     4681 gattttctgt ggaatgagta cgccgactgg tatatcgaga tatcgaaaat cgcgctgaac
     4741 tctacagatg aaaatga
//
LOCUS       DASRTJ010000663         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_138014,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000663 DASRTJ010000000
VERSION     DASRTJ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138014"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFF70_13465"
     CDS             complement(<1..1300)
                     /locus_tag="VFF70_13465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45742.1"
                     /translation="MTSQNMNHSSLISVIIPVFDGGENFQRCIDALRACDPPPHEVIV
                     VDDGSTDESRRVANERGARVLMTDRPRSGPAMARNIGANAATGDVVLFIDADVAVQPD
                     VIGRIAHNFEADSDLAACFGSYDEQPAAQNFLSQYKNLFHHYVHQSAQADASTFWAGC
                     GAIRRDLFLKVGGFSETYPRPSIEDIELGYRLKAAGCKLQLDKGIQCQHLKRWTWRSL
                     LRSDILDRGMPWTELILRDGAFVNDLNLQTHNRISVVTVYLLLLSLAIGVLQPIGWLA
                     SAGLAIILLRLNLKVYCFFKAQRGLFFTLRVVPTHWLYYVYNGISFSIGAARHIFSAD
                     QSFERRKRWPLRVMILVMFIGAALRFINLGRDSFWYDELLQVNIAANHLPLLLSKMAE
                     NSAMPLDYLLTHGMLLLGRSEFWLRFPAAMWGLLTLPLMYQ"
     gene            complement(1391..2617)
                     /locus_tag="VFF70_13470"
     CDS             complement(1391..2617)
                     /locus_tag="VFF70_13470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45743.1"
                     /translation="MRRWLHWLTLPIAAILLLFNLRRLIFTFVALFNKSAPEIAASDS
                     SLIPHPSSLILIPCRNEAATLLGLIDCLDRVEYPADRLHIALIDDGSIDATGEIIDQA
                     ARARSNWTAIHLAINRGKPKALNDALSQIEFGEVIVVYDVDHRPEPDSLVKLIAAFDD
                     PFVAGASGRTKPINPTASWPAYYATVESLVHQLITMRAKDQLDLAPALLGSNCAYRRS
                     ALLQAGGFRSGALLEDSDLTVRLTRFGYRLRFVPESIAWHQMPESIEGYIEQHMRWSR
                     GFNDVARDHLIDLLRDRAVPPRVRIESAIFALGYLDRLALLSALGMQLIDRIFGSGFY
                     FTKSILQLSLIMPLIQIAAIFKSERVSISMWRRFPVIPLLFGLDIFVAVRSMLESLLN
                     RPRVWTKTQRLQELNK"
     gene            complement(2617..>3036)
                     /locus_tag="VFF70_13475"
     CDS             complement(2617..>3036)
                     /locus_tag="VFF70_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45744.1"
                     /translation="DAQVTRFVYAALDVVKRVFNSPLPPAEITAQLRAGCPLRLQQWI
                     DRYALSDVAQLDYRQPDKGTAYVLTNLAAQSIGEKFGIVRYALLPPRQFIMLKYKLKE
                     PWQAIPFYVPYILSRTFDTLKSFLRAVTRSARLEGRA"
BASE COUNT          797 a          804 c          823 g          612 t
ORIGIN      
        1 tctgatacat gagcggcagc gtcagcagtc cccacatcgc cgccggaaaa cgcaaccaaa
       61 actcactgcg cccgaggagc aacatcccat gcgtaaggag gtaatcgagc ggcatcgccg
      121 aattttcagc catcttggac agcagcaacg gcagatggtt cgcggcgatg ttgacttgca
      181 gcagttcatc gtaccagaaa ctatcacggc ccagattgat gaagcgcaag gccgcgccga
      241 tgaacatcac cagaatcata acccggagtg gccagcgctt tcgccgctcg aaagattggt
      301 cggcgctgaa gatgtgtcgc gcggctccga tcgaaaatga gatgccgtta taaacatagt
      361 acaaccaatg ggttggcacg actctcaagg tgaagaacaa accgcgctgc gctttgaaga
      421 aacaatatac cttcaaatta agccgcagca aaataatcgc tagcccggcg gacgccagcc
      481 aaccgatcgg ctggaggact ccgatcgcca gcgacagcag cagcagatac accgtgacga
      541 cactgatgcg attgtgcgtc tgcaaattca gatcgttgac gaatgcgcca tcgcgcagaa
      601 tcaattcggt ccacggcatg ccgcgatcaa gaatatcgga tcgcaataac gatcgccagg
      661 tccagcgttt gagatgctga cattgaatcc ctttatccag ttgcaatttg cagcctgcag
      721 ccttcagtcg atagcccaac tcaatatctt caattgaagg acgcggatac gtttcactga
      781 agccgcctac tttcaaaaag aggtcgcgcc ggatcgcgcc gcagcccgcc caaaatgtgg
      841 aggcgtcggc ctgtgccgat tgatgcacgt aatgatgaaa caaattctta tactgcgaca
      901 aaaaattttg cgcggcaggc tgttcatcgt acgagccgaa acacgcggcg agatcagaat
      961 cggcttcaaa gttgtgcgca atacggccaa ttacatctgg ctgaacggct acatccgcat
     1021 cgatgaacaa tacaacatcg cccgtagctg catttgcacc gatgttacgc gccatcgccg
     1081 ggccgctgcg gggtcgatcg gtcatgagga cacgtgcacc gcgttcgttc gcgacacgtc
     1141 gagactcatc ggtcgatcca tcgtcgacga cgatgacttc atgcggcggc ggatcgcagg
     1201 cgcgcagagc atcaatgcag cgttgaaaat tttcgccgcc gtcgaagacc ggaatgatga
     1261 cgctgatgag tgacgagtga ttcatatttt gtgacgtcat tgcgagccgc gaagcgacga
     1321 agcaatctca gactcgatca ggagattgct tcgtcgcaaa aagatgctcc tcgcaacgat
     1381 ggatcaattt tcatttattc aattcttgaa ggcgctgtgt cttcgtccag acccgcggcc
     1441 gattgagcaa tgattcgagc atggaacgca ccgccacaaa aatgtccaag ccgaataaaa
     1501 ggggaataac tggaaagcgc cgccacatgg atatcgagac acgttctgat ttgaagatgg
     1561 ccgcaatttg aattaacggc attatcaaag aaagttgcaa aatggacttt gtaaagtaaa
     1621 atccactgcc aaagattcga tcgatcagct gcatgcccag tgcgctgagc agcgccaacc
     1681 gatcaagata accaagcgcg aagatggccg attcgattcg cacgcgcggt ggcacagctc
     1741 gatcgcgcag cagatcgatc agatgatctc gcgccacatc gttgaagccg cgcgaccaac
     1801 gcatatgctg ctcgatgtaa ccctcgatcg attccggcat ttgatgccac gcaatcgatt
     1861 ccggcacaaa gcgcaagcga taaccgaatc gagttaagcg cacagtgaga tcgctgtctt
     1921 ccaacaaagc gcccgatcga aaaccacccg cttgcaggag cgcgctgcgt cgatacgcgc
     1981 aattcgatcc caacaacgcc ggagccaaat ccagttgatc cttcgcgcgc atggtgatca
     2041 gttgatgcac gaggctttca actgtcgcat aatacgcggg ccaactcgcg gttggattga
     2101 tcggcttggt ccggccgctc gcgcccgcaa cgaagggatc atcaaacgcg gcgatcaact
     2161 ttaccagtga atcgggttcg ggccgatgat cgacatcata cacaacgatc acttcgccga
     2221 attcaatttg actcaaggcg tcattcaacg ctttaggttt gccgcgattg atcgctaaat
     2281 ggatcgccgt ccaattcgat cgggcgcgcg cggcttgatc gatgatttca cctgtggcat
     2341 cgatggagcc gtcgtcgatc aacgcgatat gcaagcgatc ggctggatac tctactcgat
     2401 cgaggcagtc gatcaggccg agcaaggtcg cggcttcgtt gcggcaaggt atcagaataa
     2461 gggatgaggg atgagggatg agggatgaat cggatgcggc gatctcaggt gcagatttgt
     2521 tgaacaaggc aacaaatgta aatatgagcc gacgtaaatt gaagagcagc aagattgcgg
     2581 cgatgggcaa ggtgagccaa tgaagccagc ggcgcattag gcccgacctt caagccgagc
     2641 ggatcgagtg accgcacgca ggaaagactt cagcgtatcg aaggtgcggc tcagaatata
     2701 aggcacatag aatggaatgg cttgccacgg ttccttcaat ttatatttga gcataatgaa
     2761 ttgacgcggc ggcaagagcg cgtaccggac gataccaaat ttttcgccga tcgattgcgc
     2821 ggccagattg gtcaacacgt aggctgtgcc tttatccggc tgacgataat cgagttgcgc
     2881 gacatcactg agcgcatacc gatcgatcca ctgttgcaat cgcagcggac aaccggcgcg
     2941 caattgtgct gttatttcgg cgggtggtag gggcgagttg aagacacgct tgacaacatc
     3001 aagtgccgca tatacaaagc gagtgacctg tgcgtc
//
LOCUS       DASRTJ010000664         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_210475,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000664 DASRTJ010000000
VERSION     DASRTJ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210475"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..283)
                     /locus_tag="VFF70_13480"
     CDS             complement(65..283)
                     /locus_tag="VFF70_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45745.1"
                     /translation="MDKFFSRILDRISDTIANHRGMPILICLPMVIFNYILRVIPGTQ
                     LGFVETTDLFLHLAVFIGLLGVLLGEAL"
     gene            complement(299..1663)
                     /gene="hflX"
                     /locus_tag="VFF70_13485"
     CDS             complement(299..1663)
                     /gene="hflX"
                     /locus_tag="VFF70_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZY45746.1"
                     /translation="MDFQEVAITKHREAYPTESPSPRTFMIGVELRGAHGLLSVEDSL
                     DELRALADTAGLNIVGSMTQKLNTIVPATFIGTGKVEELKVWQAELGVGVFLFDDELS
                     PRHQRNLEKELGEEVRVLDRTALILDIFAQHARTREGALQVELAQYEYRLPRLTRQWT
                     HLARQSGGGSGRAGTAGVGLRGPGETQLEVDRRQIRSRITFLKSELEGVRLHRQQYRA
                     KRRRAQIPVVAIVGYTNAGKSSLLNALVDASGSKQTPVLAEDKLFATLDPTTRRLRLP
                     GGKFALFTDTVGFIQKLPTQLVAAFRATLEEIEEADLLLHVVDVTHPNAIEQAKSVEQ
                     TLAELNITDIPMVVALNKIDRLDDGQLQDLSSGQFPHSVAISALHQQNLEQLLKLIEH
                     ELYENLAPVELRIPYRRTDLVALFHDNGRVDKEEYGEKDIKLAGRLPARYLAAFRQFM
                     VKAK"
BASE COUNT          392 a          491 c          490 g          418 t
ORIGIN      
        1 gtgctattaa taaaaagagg catcgagtca aactcgatgc ctcttcgtat gcaaagacct
       61 cacttcatag agcttcgccg agcaatacgc ccagcaatcc gatgaacacc gccaggtgta
      121 aaaacagatc ggtcgtttca acaaagccca actgcgtgcc ggggatcacc cttaagatat
      181 agttaaagat caccatcggt aaacaaatca gaatcggcat gccgcgatga ttggcaatag
      241 tgtccgaaat gcgatcgagg atgcgcgaga aaaacttatc catagcgatc tcctgaggtt
      301 acttcgcctt gaccatgaat tgccggaaag ccgccaagta acgggccggg aggcgcccag
      361 ctagtttgat gtctttctcg ccgtactctt ctttatcgac acggccattg tcgtgaaaca
      421 gcgccactaa atccgtgcgc cgatacggga tgcgcagctc gacgggtgca aggttctcat
      481 acagctcatg ttcgatcagc ttgagtagct gctcgagatt ttgttgatgc agcgccgaga
      541 tggccacgct gtgcggaaat tgaccggacg agagatcctg taattgaccg tcatccaatc
      601 ggtcgatctt gttgagtgcc acaaccatcg ggatatcggt gatattcaat tcggccaacg
      661 tttgctcgac tgatttcgct tgctcgatcg cgttgggatg tgtgacatcg acaacgtgca
      721 gcagcagatc ggcttcttca atctcttcga gcgtggcgcg aaaagcggcg acgagttgcg
      781 tgggcagctt ttgaatgaag ccgaccgtgt cggtaaacag cgcgaacttt ccgccgggca
      841 atcgcaagcg ccgcgtggtg ggatcgagtg tggcgaacaa tttatcttcg gccaacaccg
      901 gcgtttgctt tgaaccgctg gcatctacta aggcattcag caatgacgac ttgcccgcgt
      961 tggtgtagcc gacgatcgct acgaccggaa tttgcgcgcg gcggcgcttg gcgcgatatt
     1021 gctggcgatg cagccgcacg ccttccagct cgctcttcag gaacgtgatg cgcgatcgga
     1081 tctgccggcg atcgacctcg agctgcgttt cgcccgggcc gcgcagaccg acacccgccg
     1141 tgcccgctcg gcctgatcca ccgccggatt gtcgagccag atgtgtccac tgacgagtca
     1201 gccgcggcaa tcgatattcg tactgtgcca attcaacttg cagtgcgcct tcgcgggtac
     1261 gggcatgctg cgcgaaaata tcgaggatga gcgctgtccg atcgagcacg cggacttcct
     1321 cgcccaattc tttttcaagg ttgcgctgat ggcgcggcga taattcatcg tcgaacagga
     1381 agacaccgac gcccaactcg gcttgccaga ctttaagttc ttcaactttg cctgtgccga
     1441 taaaagtcgc cggcacaatc gtgtttaact tttgcgtcat cgagccgacg atgttcaggc
     1501 cggcggtatc cgctaaggca cgcagttcgt ctaacgagtc ttcaacggag agcaagccgt
     1561 gtgctccgcg cagctcaaca ccgatcatga atgtgcgcgg gcttggtgat tctgtggggt
     1621 aggcttcacg atgtttggta atggcgacct cttggaaatc caaaaggttt taaaaacctt
     1681 ttggatttag cgttcattct tccttttcgt accgcaagtc catatcttcg cgaccgtgca
     1741 gcgctgcttg cagggcttcg accaatgtcg gatcgatgcc ctgttcttgc a
//
LOCUS       DASRTJ010000665         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_291579,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000665 DASRTJ010000000
VERSION     DASRTJ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291579"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VFF70_13490"
     CDS             <1..1078
                     /locus_tag="VFF70_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZY45747.1"
                     /translation="KEYIARGTYIYPPAPAMRLITDIFKFCRIEVPRWNTISVSGYHM
                     REAGSTAVQEVAFTLANGIAYVQAALDAGLDVDEFAEQISFFFNAHNNFLEEIAKFRA
                     ARRVWARIMRDRFKAKNPNSMKLRFHTQTGGSTLTAQQPENNVVRVTLQALAAVLGGT
                     NSLHTNSRDEELWLPTEESVQIALRTQQIIAYESGAADSIDPLAGSYLIEHLTDEIEV
                     KAKEYIDRIDQLGGTLRAIERNYIQGEIAEAAYAYQRSIERQEQIVVGLNQFQTNEQL
                     HLDRLRVDPSFEFEQRSRLAEVRKNRDNNRVSELRSYLEAAAKSTENLMPPILACVEG
                     DVTLGEICNTLRGVFGEYQPSVGI"
     gene            1090..1662
                     /gene="mce"
                     /locus_tag="VFF70_13495"
     CDS             1090..1662
                     /gene="mce"
                     /locus_tag="VFF70_13495"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZY45748.1"
                     /translation="MTIKRIDHIAIVVDNMEGALEFWEDALGLQMNHMEEVADQESIV
                     AFLPSGHSEVELVEPTSDSSGVAKYLAKRGPGIHHICFEVDDIDLALARMKAKGVKLI
                     NETPTIGTGGKKIAFIHPSSTHGVLVELYELTPEEPMRRRRLIREWRRRIDTQRAVAS
                     AGLRTFVKTWRQQIRRSNDREKKEIVLKQN"
     gene            1855..>2628
                     /locus_tag="VFF70_13500"
     CDS             1855..>2628
                     /locus_tag="VFF70_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZY45749.1"
                     /translation="MDFLHGLNPQQRRAVEAPDGPALVLAGPGSGKTRVLTQRVAYLV
                     EGRGISPRHILAVTFTNKAAREMRERLEKILSPTRAVELTLGTFHSICARWLRRDAPE
                     LGLNREFVIYDQDDQTSLVKQALKDLNVDEKRYTPSAMLNAISSAKNELITPENYQPP
                     SYWHEMVRRVYPRYQELLRASNALDFDDLLLEVVRLFDTKPEVLKRYQERYQYFLVDE
                     FQDTNTAQYTMLQQLSNKTHNLFCVGDEDQSIYKWRGADF"
BASE COUNT          642 a          656 c          754 g          576 t
ORIGIN      
        1 caaagagtac attgcgcgcg gcacgtatat ttatccgccc gcgcctgcga tgcgcctcat
       61 caccgatatt ttcaaattct gccgcatcga agtgccgcgc tggaacacga tcagcgtcag
      121 cggctatcac atgcgtgaag ccggatcgac ggcggtgcag gaagtggctt tcactttggc
      181 gaacggcatc gcttatgtgc aagctgcgct cgatgccgga ctcgatgtcg atgagtttgc
      241 cgaacagatt tcattcttct tcaacgcgca caataatttt ctggaagaga ttgccaagtt
      301 ccgcgcggcg cggcgtgtgt gggcgcgcat catgcgcgat cgattcaagg cgaagaatcc
      361 caattccatg aagctgcgct ttcatacgca gaccggcggg agcacgttga ccgctcagca
      421 gccggagaac aatgtcgtgc gggtcacgct tcaagcgtta gctgcagtat tgggcgggac
      481 caattcgctt cacaccaatt cgcgagatga ggagttgtgg ctgccgactg aagagagcgt
      541 gcagattgca ctgcgcacac agcagatcat cgcctatgaa tccggcgccg ccgattcgat
      601 cgatccgctg gcgggatcat atttgattga acatctcacc gatgagatcg aagtcaaggc
      661 caaagagtat atcgatcgga tcgatcagct cggcgggacg ctgagagcga ttgagcggaa
      721 ctatatccag ggcgagatcg ccgaagcggc ttatgcctat cagcgatcga ttgagaggca
      781 ggagcaaatc gtcgttgggc tgaatcagtt tcaaacgaac gagcagctgc acctcgatcg
      841 attgagggtc gatccctcgt tcgagtttga gcagcgatcg agattagcgg aagtgcggaa
      901 gaatcgggat aataatcgag tatcagaatt aagatcgtat ttggaagccg ctgccaaatc
      961 gaccgagaat ttgatgccgc ctattctggc gtgcgtcgaa ggcgatgtga cattgggcga
     1021 aatttgtaat acacttaggg gcgtgttcgg cgagtatcaa ccttcggtcg gcatctaact
     1081 ggagcaagca tgactattaa gcggatcgat cacattgcaa ttgttgtcga taacatggaa
     1141 ggcgcgctgg aattttggga agacgcgttg ggcttgcaga tgaaccacat ggaggaagtg
     1201 gctgatcaag aatcgatcgt cgcgtttctg ccctcaggcc acagcgaagt ggaactcgtt
     1261 gagccgacat ccgattcgag cggcgtggct aaatatttag cgaagcgcgg gccgggtatc
     1321 catcacattt gctttgaagt tgatgatatc gatctagcat tggcgcggat gaaagcgaaa
     1381 ggtgtgaagt tgatcaatga gacgccgacg atcggcacag gcggcaagaa gatcgccttc
     1441 attcatccga gcagcacgca tggtgtgtta gtcgaactat acgaactcac tcctgaggag
     1501 cccatgcggc ggcgtcgatt gatccgcgaa tggcgcaggc ggatcgacac gcagcgcgcg
     1561 gtcgcgagcg cgggcctgcg gacgttcgtg aagacgtgga gacagcaaat tcgccgcagc
     1621 aacgatcgag agaagaaaga gattgtattg aagcagaatt gattttgtgc cgctgtcggt
     1681 atcggcgtgg tacaatgaac cttgcgaaga ttgaaaagtc ttcgcaaggt ttttattttt
     1741 agtggtgtga ccctgagccc ttcggctttg ctcaggataa actccgcgaa gagtctctat
     1801 gaattggcgt agagattctt cgcgtcgctc agaatgacgt gcgaccaggc aaccatggac
     1861 tttctgcacg gacttaatcc tcaacaacga cgcgcggtcg aagcaccgga tggaccggca
     1921 ctggtcttag ccgggccagg atctggcaag acgcgcgtat tgactcagcg cgtggcatac
     1981 ctcgtcgaag gacgaggtat ttcaccgcgt cacattctgg ccgtgacgtt cacaaacaaa
     2041 gcggcgcgtg aaatgcgcga acgcctagaa aaaattctca gccccactcg cgcggttgaa
     2101 ttgacgctgg ggaccttcca ttcgatctgc gcccgctggc tgcggcgcga tgcgccggag
     2161 ctcggcttga accgcgagtt cgtaatctac gatcaagacg atcaaacttc gctggttaag
     2221 caagccctca aagatctaaa tgtcgacgag aagcgctaca ccccatcggc gatgctgaat
     2281 gcgatttcct ctgcgaaaaa tgaactgatc acacccgaaa attaccagcc gcccagttac
     2341 tggcacgaga tggtgcgccg agtatatccg cgttatcaag aactgctgcg cgccagcaac
     2401 gcgctcgact tcgacgatct actgctggaa gtcgttcgat tgttcgacac caagcccgaa
     2461 gtgttgaagc gctatcagga acgttatcaa tactttttgg tcgatgaatt tcaagacacc
     2521 aatactgcgc agtacacgat gttacagcag ttgtctaata agacgcacaa cctgttttgc
     2581 gtgggtgatg aagatcaatc gatctataag tggcgcggcg ctgacttc
//
LOCUS       DASRTJ010000666         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_291804,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000666 DASRTJ010000000
VERSION     DASRTJ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291804"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VFF70_13505"
     CDS             <1..1833
                     /locus_tag="VFF70_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZY45750.1"
                     /translation="EVYKAYQPSLDRYVAIKLMHGFLADEKEFLIRFEREAKVVATLR
                     HPNIVQVHDFDVDNGVYYMVMEYITGETLKGRLQTLEEHGEWVSTDDAARIILAVGSA
                     LKYAHERGMVHRDVKPANVMITEDGQVILTDFGIAKIVTSSNLTASGAMVGTPSYMAP
                     EQGMGQPGDERSDIYSLGVMLYQLMIGRLPYDADTPLAVVLKHINDPLPLPRSVKPDL
                     SEPLERVILKSLAKNPTDRYQKVSELLADLRQAVGMPVDEAQSDVANGSSIKLSGATV
                     VGRVSGLTPPPRTTGTLPMPTQIAGAGAAAVVSSAQAPVIQRKSNAPVIVLAIIAAVV
                     ILGGGGLFLASQSNKAPSPTAVDTPIPATTAPTSTPDVSAFLLTFNTDQVELHDKPDL
                     TSNVLGLLPMSTQFHLRARTEDTAWLKIETPDGVGGWVDASKVDLSAISAELLTSLPT
                     ATSLTQPTDTPTPTEPPTSTPTITPTPRPTNTPGPTKPPSTPKPAGPTATPTKLVSAE
                     PLGHGFEFKFCTYDGINYTCNVTIWGSGGDGKYHFALENPDTGNWEEKTGNFVGYLMR
                     SRRCKIKTQQLRIWDESNAHLEPNITMNPDDIPSLFPGGTGCTL"
     gene            1968..2231
                     /locus_tag="VFF70_13510"
     CDS             1968..2231
                     /locus_tag="VFF70_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45751.1"
                     /translation="MSMQWEYCEMRWQVAQRYNAARPAVVFYRAPNQPWSPPDAVPEQ
                     LAMRLGLDGWELISVLSYESGESMGNTEIRWYFKRPYASVPEK"
     gene            2299..>2662
                     /locus_tag="VFF70_13515"
     CDS             2299..>2662
                     /locus_tag="VFF70_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45752.1"
                     /translation="MKKHLRIIIVDDHEVVRLGLRALLDRHPNFSVVDEAGSAKEAVQ
                     KVLQHRPEIVVMDIRLPGGNGIEACREIVAQSPETKVIMLTSFAEDDMLFEAIAAGAA
                     GYVLKQIGGDDLVRAIESV"
BASE COUNT          621 a          662 c          745 g          634 t
ORIGIN      
        1 gaagtatata aagcttatca gcccagtctc gatcgctatg tggcgatcaa gttgatgcac
       61 ggctttctgg ctgatgagaa agagtttctc attcgcttcg agcgtgaagc gaaagtggtg
      121 gccacgctgc gccatcccaa catcgtgcaa gtccacgact tcgatgttga taacggcgtg
      181 tattacatgg ttatggagta tatcaccggc gagacattga agggccggtt gcaaactctg
      241 gaagaacatg gtgagtgggt ctccactgat gatgcggccc ggattattct ggccgtcggt
      301 tcggcgttga agtacgcgca tgagcgcggc atggttcacc gtgatgtaaa gccggcgaat
      361 gtcatgatta ctgaagatgg tcaagtgatc ttgacggact tcggcattgc caagattgtc
      421 acttcttcca atctcaccgc cagcggcgcc atggtcggca cccccagtta catggcgccc
      481 gagcaaggca tgggccagcc cggcgatgag cgcagcgaca tttactcatt gggcgtcatg
      541 ctgtatcaac tcatgatcgg tcgattgcct tacgacgccg acactcctct cgctgttgtg
      601 ttgaagcaca tcaacgatcc tttgccgctg cctcgatcgg ttaagcccga tctgtctgag
      661 ccgctcgaac gagttatctt gaagtcgctc gccaagaatc ccaccgatcg ctaccaaaaa
      721 gtcagtgagc tacttgccga tctgcgccag gcggtcggca tgcccgttga tgaggctcaa
      781 tcggatgttg ctaacggctc atcgatcaaa ctgagtggtg caacggttgt gggccgagtg
      841 agtggactta caccaccacc gcgcacaacc ggcacactcc cgatgccaac gcagatcgcg
      901 ggcgcgggcg cggccgccgt cgtttcaagt gcgcaagcgc cggtcattca acgaaagagc
      961 aatgctcccg tgatcgtatt agcgatcata gcggcagttg taatcttggg cggtggcgga
     1021 ttattcctcg ccagccagag caataaagca cctagcccaa cggcggtcga tactcctata
     1081 ccggcaacaa ccgcgccgac cagcacgccg gatgtgtcgg catttctgct gacatttaat
     1141 accgatcaag ttgaattgca cgacaagccc gatcttactt caaatgtttt aggccttttg
     1201 ccgatgtcta ctcaatttca cttgcgagcg cgaacggagg atactgcgtg gttgaagatt
     1261 gaaacgcctg atggtgtagg tggctgggtc gatgcgagta aggttgatct gagcgctatc
     1321 tcagctgaac tcttgacgag tttgccgact gcaacttcgt tgactcaacc gactgatacg
     1381 cccacgccaa ctgagccgcc gacgtcgacg ccaacaatca cgccaactcc aaggccgaca
     1441 aatacacctg gcccgacaaa gccaccgtca acaccgaagc ctgcaggccc gacagctaca
     1501 cccaccaagc tggtttcagc tgagccattg gggcacggat ttgagtttaa attctgtaca
     1561 tatgacggta ttaattacac ctgcaacgtc actatttggg gtagcggcgg cgatggcaaa
     1621 tatcacttcg cgctcgaaaa tcctgataca ggaaattggg aggagaaaac cggaaatttt
     1681 gtcggctatc tcatgcggtc aaggcgttgc aagataaaga cccaacagct gcggatctgg
     1741 gacgaatcaa atgcacacct ggagccaaac attaccatga acccggatga cattccgtca
     1801 ctctttcccg gtgggactgg ttgtacattg taggttttag ctactaaaat agggttagag
     1861 gatttccgtc gccaaaaatc aacaagcgcc gaagttgtgt catgaccttt gaatcggatt
     1921 tgtagatcga ttcgaggtca tgattttgtc gcgtatacgg aggttgcatg tccatgcagt
     1981 gggaatattg tgaaatgcgt tggcaggtcg ctcaacgtta taacgcggcg cgacccgccg
     2041 tcgtttttta tcgcgcgcct aatcaaccgt ggtcgccacc ggatgctgtg ccggagcaat
     2101 tggccatgcg cttggggctg gatggttggg aattgatatc ggttctgtcg tacgaaagcg
     2161 gtgaatcgat ggggaatacc gagattcgtt ggtatttcaa acggccgtac gccagcgttc
     2221 cagaaaagta atgttcgctg tcgagcgcgc gagttgagat aaattcaagg ttaggttgtg
     2281 cttgattgtg aggctggatt gaaaaagcat ttgcgaatta tcattgtcga tgatcatgaa
     2341 gttgtgcgtc ttggactgcg ggcgttgctc gatcggcatc ccaatttttc ggtggttgat
     2401 gaagcgggtt ctgccaagga agccgtacaa aaagtcttgc agcatcgacc tgagattgtg
     2461 gtgatggata ttcgcttacc cggtggtaac ggtatcgagg cttgtcgcga gattgtggca
     2521 caatcacccg aaactaaggt gattatgctg acgtcgttcg cggaagatga tatgttattc
     2581 gaggcaatcg cggccggcgc agcgggttat gtgcttaagc aaattggtgg cgacgatctg
     2641 gtgcgcgcga ttgaatcggt cg
//
LOCUS       DASRTJ010000667         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_291872,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000667 DASRTJ010000000
VERSION     DASRTJ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291872"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFF70_13520"
     CDS             complement(<1..282)
                     /locus_tag="VFF70_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45753.1"
                     /translation="MRGRFVLSLLLVLVIVAGAIGLGTYAYNSGIAQGLAQSGRIAAP
                     DGGAVVPYYAPYYHQPFGFGFGGFLFSCFFLLLIIGLVRAIFGRRHWGMH"
     gene            complement(415..1176)
                     /locus_tag="VFF70_13525"
     CDS             complement(415..1176)
                     /locus_tag="VFF70_13525"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45754.1"
                     /translation="MSHAALRVEHVGHIFDDQNGQVEALIDISFEIAPSEFVCLVGPS
                     GSGKSTLPRILAGLIQPQQGHVWLDGQLVESPGPSIGVVFQKANLMPWRTAIENIALP
                     LELNGVASNEVLRRSNELIELVGLNGFANAYPHSLSGGMEQRVAIARALIHNPSILLL
                     DEPFGSLDALTRERMALELTRIWSSQKTTVVMVTHSIQEAVFLSDRVFVMTDRPGRIA
                     ADFPVNFDRPRDMSLLQTERFAEIAGRVREAISAT"
     gene            complement(1173..2165)
                     /locus_tag="VFF70_13530"
     CDS             complement(1173..2165)
                     /locus_tag="VFF70_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY45755.1"
                     /translation="MKKFFLLLSIAALVLAGCGNTAAAPTAPAQPTHLQLPMGYIANV
                     QFAPFYVAVDKGYFAREGIAIDFDYRFETDGMKLVGANQLPFAVVSGEQVPLARSQGL
                     PVTYVMQWYQNFPIGVVAISDKNIKTPADLIGRSVGLPGFFGASYIGWRGLLYKANVN
                     EADIKTQDIGFTQVAALQQGKVDAIVGYTNNEPLQLLAAGLKIDVIQVSDYVSMVANG
                     IVTNDSTIKNNPQLVTGFLRALVQGLQDVKNNPDEAFTISTKYVDGLGKDPKNDALQM
                     QVLYASIKLWTIDGRSNPKSWDTTQEVLVNMGLLTAKIDPAQLFTNDFLPMAGK"
BASE COUNT          538 a          604 c          598 g          524 t
ORIGIN      
        1 gtgcataccc caatggcgac gcccgaagat ggcgcgcacc aggccgatga tcaaaagcag
       61 gaagaagcag ctgaacaaaa atccgccgaa gccgaaacca aacggttgat ggtaatacgg
      121 cgcataatac ggaaccactg cgccaccatc cggcgcggcg atccggccgc tttgcgccaa
      181 accttgcgcg atgcctgagt tgtacgcgta ggtgcctaaa ccgatcgcgc ccgcgacgat
      241 cacgagcacc aacaacagtg ataaaacaaa tctgcctcgc ataatactcc ttcacttgct
      301 gaatggattc tgaagtgatt cttcagtctg aagagagtat cgcgccggag tgtgaaccaa
      361 ttgtgaatga gcgatggagg gattgaagat gattgtgcgg gaaagactgt ggagttatgt
      421 ggcgctaatt gcttctctca cccgaccggc gatctcggcg aagcgttccg tttgcagcag
      481 actcatatcg cgcggtcgat cgaaattcac ggggaagtcg gcggcaattc ttccgggccg
      541 atcggtcatg acgaaaactc gatcgcttaa gaacacggct tcttgaatgg aatgggtgac
      601 catgaccacg gttgtttttt gcgaagacca gatgcgcgtg agttcgagcg ccatgcgttc
      661 gcgcgtcagc gcatcgagcg agccgaatgg ttcatccagc agcaagatcg atgggttgtg
      721 gatcagcgct cttgcgatcg ctacgcgctg ctccatgccg cccgataaac tgtgaggata
      781 tgcattcgca aagccattca aaccgacaag ctcgatcaac tcatttgatc ggcgcaggac
      841 ttcgttcgag gcgacgccat taagttcgag cggcagtgca atgttttcga tcgcggtgcg
      901 ccatggcatg aggttcgctt tttggaacac cacaccgatc gacgggccgg gcgattccac
      961 taattggcca tcgagccaca cgtggccttg ttggggctga atcaatccgg ccaagatgcg
     1021 cggcagcgtc gatttgccgc tgcccgatgg tccgaccagg cacacgaact cgctcggtgc
     1081 aatctcgaac gaaatatcga tcagcgcttc gacttgtcca ttttgatcgt cgaagatatg
     1141 tccgacatgt tcgacgcgta atgcggcgtg actcatttac ccgccatggg taggaagtca
     1201 ttggtaaaca actgcgcggg atcaatcttg gcggtgagca gacccatatt caccaaaact
     1261 tcctgcgtcg tatcccacga cttaggattc gatcggccgt cgatcgtcca caatttgatc
     1321 gaggcataca acacttgcat ttgcaacgca tcatttttgg gatctttacc cagcccgtcg
     1381 acatacttgg tcgagatcgt gaaggcttca tccggattat tctttacatc ctgcaagcct
     1441 tgtactaatg cgcgcaggaa gccagtcacc aactgcggat tgtttttgat cgtgctgtca
     1501 ttcgtcacaa tgccattcgc caccattgac acgtaatcac tgacttgaat cacatcgatt
     1561 ttcaaaccgg ctgcaagcaa ttggagcggt tcgttattcg tataaccgac aatcgcatcg
     1621 accttgccct gctgcaaggc ggcaacttgc gtgaagccga tatcctgcgt tttgatgtcc
     1681 gcttcattga cgttggcctt atacaacagg ccgcgccagc cgatataact tgcgccgaag
     1741 aagcccggca gtcctactga tcttccgatc aaatccgccg gcgttttgat atttttatcc
     1801 gatatggcca cgacgccgat cggaaaattc tgataccact gcatcacata tgtgaccggc
     1861 agtccttgag atcgggcgag tggcacttgt tcgccgctga ccaccgcgaa cggaagttga
     1921 tttgcgccga ctaacttcat gccatcggtt tcaaaacgat aatcgaaatc gatcgcgatg
     1981 ccttcccggg cgaagtaacc cttgtcgact gcgacataga atggcgcgaa ttgcacgttg
     2041 gcgatgtaac ccatcggtag ttggaggtga gtgggttgag ccggagcagt cggcgctgcg
     2101 gcggtgtttc cgcaacccgc taggaccagc gcggcgatcg agagtaataa gaaaaacttt
     2161 ttcatctgaa tttttcctca acaaagattg atttgtcatt tcgacgagcg gagcgaggag
     2221 aaatctccgt tcaagatcga gatttctcgc ttcgctcgaa atga
//
LOCUS       DASRTJ010000668         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_210687,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000668 DASRTJ010000000
VERSION     DASRTJ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210687"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1039)
                     /locus_tag="VFF70_13535"
     CDS             complement(149..1039)
                     /locus_tag="VFF70_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZY45756.1"
                     /translation="MACYPLLQVDAFTTRALSGNPCAVVFDADDLDDRSMLAIAREMN
                     LSETAFVRRSVVADFGVRYFTPAEEIPLAGHPTIATTFALIETGRLKLTCDLTQITLE
                     LKVGPIRVEIFSHNNTVDQIVMTQKKPQFLATHRSEDVLPLFDLTPEDALPNVPIQTV
                     SSGTPQLMIPIRDHDALRRAQIDVNAYIAYRNKSDFFSPHLFCLQGVTDRGRTFARHF
                     GVPPDTLEDAFTGSATGGMAAYIWHYGLIDQPTFIAEQGHWMNRPGEATVEIIGARDN
                     IETVKVGGSAVTVFRGELVI"
     gene            complement(1047..1385)
                     /locus_tag="VFF70_13540"
     CDS             complement(1047..1385)
                     /locus_tag="VFF70_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZY45757.1"
                     /translation="MDCLFCKIAGGQRPAKIIYRSSDVVAFHDISPQAPTHILVIPVK
                     HIASAREVQSGDADLIGHMITAAVQIAKEQNLDRGYRFVINTGSDGGQSVDHLHIHIL
                     GGRQMRWPPG"
     gene            complement(1385..2047)
                     /locus_tag="VFF70_13545"
     CDS             complement(1385..2047)
                     /locus_tag="VFF70_13545"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HZY45758.1"
                     /translation="MKSKQLMLSVLILWLPSACSNNSSGSSTVPAPPSAFTPLPYTEN
                     FDNPASGWKIASDESIKIQYQNSALNFTLDALNAIAWSTPNQKFGDFTFEVDATQVDG
                     PDDNGYGVIVRYVDDRNFYRLDISGDGYFDVLKYKAGIWIKLQDWTESTAIQPGATTN
                     HLRVAAQGNQFTFSVNDQIVKTFTDDDFRQGDIGLSAGTFFDHAGVQVAFDNLKVTGV
                     KP"
BASE COUNT          505 a          553 c          593 g          512 t
ORIGIN      
        1 atgacaatga gaaaaagtaa agctgcaatg cctactgtga tcgcgaggca acccgaaaat
       61 gaaaatcgtc gtcgatgtga tacagattca gcgatcataa tgcgaaagcc ctggacaccc
      121 gccttcgcgg gtgtaacaat acgcgtgatt agatcaccaa ctcgccacga aaaacggtga
      181 cggcactgcc gccgactttg acggtctcaa tattatcacg cgctccgatg atctcaaccg
      241 tcgcctcgcc gggtcgattc atccaatgtc cctgctcggc gatgaaggtc ggttgatcga
      301 tcaagccgta atgccaaatg taggcggcca tgccgcccgt cgccgagccg gtgaatgcat
      361 cttccagcgt atcgggcggc acgccgaaat gccgcgcaaa ggttcggcct cgatcggtaa
      421 cgccttgcaa gcaaaacaga tgcggactga agaaatcgga tttgttccga taggcgatgt
      481 aggcgttcac atcgatctgc gcgcggcgta gcgcgtcgtg atcgcggatt ggaatcatta
      541 actgcggtgt gccactgctg acagtttgga tcgggacatt gggtagagca tcttccggcg
      601 tgagatcgaa cagaggcagc acatcttcag atcgatgcgt cgctaagaac tgcggtttct
      661 tctgggtcat cacaatttga tcgacggtgt tgttgtgcga aaaaatttca acccgaatcg
      721 gcccgacttt cagttcaagc gtgatttgtg tgagatcgca ggttaatttc aagcggccag
      781 tttcgatcag tgcgaatgtc gtggcgatcg tcggatggcc agccagtgga atctcttcgg
      841 cgggtgtgaa gtatcgcact ccgaagtcag ctacaaccga tcggcgcaca aaagccgttt
      901 cggataaatt catctcacgc gcaatcgcca gcatcgatcg atcgtccaga tcgtccgcat
      961 cgaacacgac tgcgcacgga ttgccgctta aggcgcgagt ggtgaaggca tcgacttgca
     1021 aaagtggata acacgccacg ctgattctat cccggcggcc agcgcatttg tcgtccgcct
     1081 aaaatatgga tgtgtaaatg atcgaccgat tggccgccgt ctgatccagt gttgatcaca
     1141 aagcgataac ctcgatcgag attctgctct ttggcgattt gaactgcggc ggtgatcatg
     1201 tgcccaatca gatctgcatc gcccgattga acttcgcgag ccgacgcgat gtgcttgacg
     1261 ggaatcacta agatgtgagt gggcgcttgc ggagaaatat catgaaaggc cacaacgtct
     1321 gaactgcgat aaataatttt ggccggtcgc tgcccgccag cgattttaca aaagaggcaa
     1381 tcgatcatgg cttcacaccc gttactttaa gattatcgaa cgcgacctgc acgcctgcat
     1441 ggtcgaagaa cgtgccggcc gatagcccaa tgtcgccttg tctgaaatca tcgtccgtaa
     1501 aagttttgac gatttgatcg ttcacgctga aggtgaattg attgccctgc gcggcaacgc
     1561 gcagatgatt tgtcgttgcg cctggttgga tagctgttga ttccgtccaa tcctgaagct
     1621 tgatccaaat gcccgctttg tatttcaaca cgtcgaagta gccgtcgccg ctgatgtcca
     1681 atcggtaaaa attgcgatcg tccacgtagc gcacgatgac gccgtagccg ttatcgtccg
     1741 gtccatcgac ctgcgtcgca tcgacctcga atgtgaagtc gccgaatttt tggttgggcg
     1801 tcgaccaggc gatcgcgttg agcgcatcaa gcgtaaagtt cagcgcactg ttttgatatt
     1861 ggatcttgat cgattcgtcc gaggcgatct tccaaccgct ggccggattg tcgaaatttt
     1921 ctgtataggg caggggcgta aacgcggagg gaggtgcagg gaccgtactg ctgccgctgc
     1981 tgttgttgct gcaggcggaa gggagccaca gaatgagcac tgaaagcata agttgttttg
     2041 atttcatctg atgctcagta actcccacct agcctcccct attgcttcgc aacaagggag
     2101 agatttactt ccttctccag tcgacatctt tttgttaaca ggggaagggc aggagatgcg
     2161 gtt
//
LOCUS       DASRTJ010000669         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_138572,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000669 DASRTJ010000000
VERSION     DASRTJ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138572"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..795
                     /locus_tag="VFF70_13550"
     CDS             25..795
                     /locus_tag="VFF70_13550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45759.1"
                     /translation="MNPNDLLTLHDRQQRIDITYHGMRREVLPDVVRHVGLEDKWGIV
                     LYSRLKTENADQVIEDQIDYFASLGLNFEWKFYEHDTPVDLRERLIAQGFAPDEPEAL
                     MILDIDHAPAILLRPIALDVRRITEPDQVPSLMKVLEQVWGSTNDWLGQRLMTDLVQD
                     TDHLSVYSGYVDDRAVCAAWIDFHDNQFAGLWGGTTLEEFRGRGFYTSLLAIRLQEAK
                     SRGIRFLVLDASPMSRAIVEKHGFQFVEYTQPHKYHSK"
     gene            complement(872..2776)
                     /locus_tag="VFF70_13555"
     CDS             complement(872..2776)
                     /locus_tag="VFF70_13555"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY45760.1"
                     /translation="MKIRVLIVVAISFLLTSHFSSAAPASIYTVNSTLDLPDADLTDN
                     ACATQPPNSVCTLRAAVMQANAHAGLDFIALPAGTYKLTRVGYDSNAAYGDLDSTDDL
                     TIYSTGGSVVINANGAVTFDRAFEVVAKSLNLSDLTIRNGNTPSYGGAIYSHGDLTLY
                     NVSVQTNTAFLDGGGIYADNSNLRVSNSTLQGNTSQASGGGIAFAGASPNPPTLSIFD
                     STLANNRALGAGSSGGGAYAYTAKVLIQNSTFRNNLAGEGGALQYTAAVVGVRLDMTG
                     TLIISNTASSVSGGGLYFYGNTSIVDSQIISNVAPSAGGLYAYFGTHVLNNVRITNNK
                     ATSYACGGIQVSSGNLYLTRGWVQYNTAPSLGGGLCTGGAGSIQMMITDTNILNNSVA
                     NGEGGGVFASNKFVALRSAIYNNFATRGGGVYAMGRAVIEDSTVSGNSAGRDGAGVYA
                     APDSNVQFNSATIASNHARTAGLFGGGTGGGIYISNTAVVSTANTLFDHNTNSDTAFS
                     FSDCKGALVSQGYNFISTNEGCTITGDPTGNQIGGSSPFNLIDPKIDPLALVFGTTPA
                     HLLHADSPLIDVGPPIGCQSALPIGFLFLDQIGHFRALDGNGDMVGLCDIGAVEYFDV
                     KRVYLPLTRK"
BASE COUNT          724 a          823 c          772 g          681 t
ORIGIN      
        1 atatagacgc gatcaataac gcgcatgaat ccaaacgatt tgctcaccct tcacgatcga
       61 caacaacgca tcgacatcac ctatcacggc atgcggcgcg aagtcctgcc cgatgtcgtg
      121 cgacatgtcg gcctcgaaga caaatggggc atcgtgttgt attcacgcct caagaccgag
      181 aacgccgatc aggtgattga agatcagatc gattacttcg ccagtctcgg cctgaatttc
      241 gaatggaaat tttacgagca cgacacgcca gtcgatctgc gcgagcgatt gatcgcgcaa
      301 ggcttcgcgc ccgacgaacc cgaagctttg atgatactgg acatcgatca cgcaccagcg
      361 atcttgttgc gaccgatcgc gcttgatgtc cgacggatta cagaacctga tcaagttcca
      421 agtctaatga aagtgctgga acaagtgtgg ggcagtacga atgattggct gggtcagcgc
      481 ttgatgaccg atttggttca ggacacggat cacctcagcg tctacagcgg ctacgtggac
      541 gatcgagccg tgtgtgctgc atggatcgat tttcacgaca atcagttcgc aggcttatgg
      601 ggcggaacga ctttagaaga atttcgaggc cgaggttttt atacttcact gttggcgatt
      661 cgattgcaag aagccaagtc gcgcggcatc cgctttctcg tgttggatgc cagccccatg
      721 agccgcgcga ttgtggagaa acatggcttt cagttcgtgg aatatacaca gccgcataag
      781 tatcactcaa agtaaaacct cgcaggtttt tggaaacctg cgaggtttgt tctgacttct
      841 cagctctgat cactgatctc tgatttactg atcacttcct cgtcagcggc aaatacactc
      901 gtttcacatc gaagtattcc acagcgccga tgtcgcacag cccaaccata tcgccattcc
      961 catcaagcgc acggaagtgg ccaatctgat cgagaaacag aaaaccgatc ggcagtgccg
     1021 attgacaacc gatgggtggc ccgacatcga tcaacggact atcggcgtgc agcagatgcg
     1081 cgggcgttgt gccgaagaca agcgccaaag gatcgatctt gggatcgatc aaattgaacg
     1141 gagatgatcc gccaatttga ttgccggtag ggtcgcccgt gatcgtacag ccctcgttgg
     1201 tgctgatgaa gttatagcct tgcgatacaa gcgcaccttt gcaatcgctg aaagagaagg
     1261 cggtatcgga attagtattg tgatcgaaca acgtgttcgc cgtgctgacc acggctgtat
     1321 ttgagatgta tatcccaccg cctgtgccgc cgccgaacaa gcctgcagta cgagcatggt
     1381 tgctggcgat tgtcgcgctg ttgaattgca cgttagaatc gggggccgca taaactcccg
     1441 caccatcgcg cccggcgcta ttgccactca cggtcgaatc ttcgataaca gcccgaccca
     1501 tcgcatatac cccgccacca cgagtcgcaa agttattgta gatcgcgctg cggagggcca
     1561 cgaatttatt gctcgcaaac acgccgccgc cttcaccatt cgccacagaa ttattgagaa
     1621 tgttcgtgtc ggtgatcatc atttggatcg aacctgcacc gcccgtgcat aacccgccgc
     1681 ctagactcgg cgcggtgttg tattgcaccc agccgcgcgt gagatagaga ttgccgctgc
     1741 tgacttgaat tccgccgcaa gcatatgatg tggccttatt attagtgatt cgcacattat
     1801 tcaagacgtg ggtgccaaag taagcatata aaccgccggc actcggcgca acgttgctga
     1861 tgatctggct atccacgatc gatgtgttgc catagaaata caacccgccg ccgctgacgc
     1921 tgctggccgt attactgata atcaacgtgc cggtcatatc caggcgcaca ccaaccacag
     1981 ccgccgtata ctgcaacgcg ccgccctcgc ccgccaaatt atttcggaag gtgctgtttt
     2041 gaattaagac cttggcggta taagcgtatg caccgccgcc agaactcccc gcaccgaggg
     2101 cacgattatt ggcaagcgtg ctatcaaaga tgctgagcgt cggcgggttg gggctggcac
     2161 cggcgaaggc aataccgcca ccggaggctt gcgatgtatt gccttgaagg gtgctattcg
     2221 aaacccggag attagaatta tcagcataga taccgccgcc atccagaaaa gccgtgttcg
     2281 tctgaacgct gacgttgtat agagtcaggt cgccatgcga gtagattgca ccgccatagc
     2341 tgggcgtatt gccatttcga atggttaggt ccgaaagatt gagcgacttc gccacaactt
     2401 caaacgcccg atcgaacgta accgcgccat tggcattgat cacaacagag ccaccggtgc
     2461 tgtaaatggt gagatcgtcg gtgctatcca aatcgccgta agctgcattc gagtcgtatc
     2521 cgacgcgagt cagcttataa gtgccggccg gcagtgcaat gaagtccagt cctgcatgag
     2581 cattggcctg catgacagcg gcccgcaagg tacacaccga gttgggtggt tgcgttgcac
     2641 aggcattgtc agtgagatca gcatccggta gatcgagcgt gctgttgacc gtataaatac
     2701 ttgccggtgc cgcgctcgaa aaatgtgagg tcaacaagaa actaattgca acgacgatca
     2761 gaactcgaat cttcatgatt acagcctccg ctgtctgcct tcactaaacg aacaattcta
     2821 agccagtcgc aaccgcaaca tttcccgatc gtcgatcctc gatcaattga tcactttctc
     2881 cctctcacca tcaagtcttc ttgttccggt aaatcttctt cacgcgcgat cagcacattt
     2941 acctcgcagg ctttgaactc cggaatcttc gccatcggat cgagcgtatc gatcgtcaac
//
LOCUS       DASRTJ010000670         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_366580,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000670 DASRTJ010000000
VERSION     DASRTJ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366580"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1323
                     /locus_tag="VFF70_13560"
     CDS             187..1323
                     /locus_tag="VFF70_13560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45761.1"
                     /translation="MRILHIYKDYFPVLGGIENHVRVLAEAGVQRGHEVTVLAASLDR
                     SLQIETLNGVKLIKTPRWINISSAPMSPAMFRWAQKIGASAGIVHLHFPYPLGELAHL
                     FSGSPAKTIITYHSDIVRQKFLRMIYRPFLWRILRKADRIIATSDRYRDTSPYISRFK
                     EKCVTIPLGSDIDHFANVDPQKVLELRKQLIPDKERTTHSILLSVGRLRYYKGLDDLI
                     RALPAIPDAITVIVGSGPMQAEWRQLVRSGGVANRVVFAGEVDDQELPLYYAASDLFV
                     LPANARAEAFGTVIVEAMAAGRAVISTEIGTGTSWINVNGQTGLVVPPHAPDQLAAAI
                     NSLLNDPDRRKKYGQAAQVRAQADFSVNKMIDRVYDVYQSLIEH"
     gene            1468..5499
                     /locus_tag="VFF70_13565"
     CDS             1468..5499
                     /locus_tag="VFF70_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45762.1"
                     /translation="MTNSSRLSALCDKMIEAGWIAAVVLVPLFFNVYSSRVFEPDKLT
                     LLRSIALTMIAAWAIKWIDQRKQPKDPDRPDRITLRTPLVLATIILVVVYLISTVFSL
                     VPAVSFFGSYQRLQGTYSTFSYIVVFAMILVHLRKREQLDRIVTAIVMTSLPIAFYGL
                     LQHGDGKNSLDPLPWGGDVTSRVASNMGNAIFVAAYLIMAFFLTLGRVVESFRTILTE
                     EESRVSDILRASAYIFVAAIQLITFGFADSRGPLLGWLPGMFIFGLVSLLMLRVSLHS
                     QTEENVEAIDSSPAAIRFPITVLDLVKAVVLTAIAVGIAAAAVFILLKSSEASEVKVK
                     LTLIAVVGLLGGLLPVLAVVGIRRTAARWAWMSLILVTISGAVGLLLINFSNIPKIVE
                     LRQSGVFGQLATLFESESGTGKVRTLIWQGSEALVTPHDPINFPDGHTDPFNVIRPLI
                     GYGPESMYVAYNNFYPSDLAHYESRNASPDRSHNETWDSLVITGVIGFIAEQFLFISV
                     FFFALKFIGWIPNRKAAMTYIALMIVGGIGGGLGLALLKGVKFFGPGWPGGVTAGIVA
                     YVLLFALFHFQISRRVYLIISLILILVVEGVIFGVTYTAEQRTIELLGGATGAAALMG
                     LFYVIGRWVFNETAGQPIDISSHVFIIIALFGGILAHYMEISFAGIAIAATRTYFWTF
                     AGLLVVTGLHWVPTSDEPAAPIASMNDAPSATLAQASPAIQSNVPRHKKKSKRVIEQS
                     RAAQSIKRNSSSALPAWLAPVMTMAVIGTLILVTLGYEFINNPPRDPNSPGFNPNPSA
                     TELIVNSLTHLPYQGNKESSGALLMFGVTWLFGGLLSLAELRRRGTLEAKDTLMASLS
                     YFGISIGMTLLVWLLLAGQLLSLGSIVHNTQITTLADLNGRVNELLTLADSVANLLSF
                     FYFVVFALMIGMAVSIMLQSRTWFGKWGTDFGWLSAIVIVPLLIIGINTTNLNNIRAD
                     TIFKQADPLKGQGQWDAAIAHYKKVSSLQPNEDFYYLWMGAAFLEKANTAPPAAADRP
                     SIITPQTTFNSIMNLDFNKSYTLNRLDTLQVALTVLTHARDLNPLNTDHSANLARLYR
                     RWSDLETDKQAKHDRLVTASDYYRQATTLSPQNAQLWNEWATVNLGLHDVDLQLNNDQ
                     GAANLQTAQDLLDHSLTLDSQYDQTYIIRGQLDRFLGKNDEAIQEYQNALKWNPDCAD
                     CWGGLSDIFSSAGNYTAVETITLAFMQTHADFVPGLRTLARNAYIPAGRLGEAIDTQS
                     QVVSLANQDPNVWDDYRVLAVMLAQAGRFPEALQAAQTGLTKAPQDKQTEMQSLIEQL
                     QQQLGVGPTLTDTVPGPNPTPTP"
BASE COUNT         1187 a         1559 c         1509 g         1310 t
ORIGIN      
        1 gatcccaaat tcaacgcggc gctcgatgcg tgttactctc gatcgactcg cggcgtgttt
       61 attgtgtatt tgttaaagaa tgctacacct tgcaaataga agggcgaggt aaccacaccc
      121 gactaatact tctttaccac cgacggagta tttacatcag cggcgccctc gtttactcta
      181 atttttatgc gcatccttca tatctataaa gattatttcc cggtgctcgg cggcattgaa
      241 aatcacgtcc gcgtactggc cgaagccggc gtgcagcgcg gccatgaagt cacggtatta
      301 gccgccagcc tcgatcgatc gctgcagatt gaaacgttga atggcgtgaa gttgatcaag
      361 accccgcgct ggatcaacat ctcctccgcg ccgatgagtc cggccatgtt ccgttgggcg
      421 caaaagatcg gcgcgagtgc gggcatcgtc cacttgcact ttccatatcc gctgggtgag
      481 ctagcgcatc ttttcagcgg cagcccggca aaaaccatta tcacgtatca cagcgacatc
      541 gtccggcaaa aatttctgcg gatgatctat cggccgtttc tctggcgcat tctgcgtaaa
      601 gccgatcgga tcatcgccac cagcgatcgc taccgcgaca cttcgccgta tatcagccgc
      661 ttcaaagaaa agtgtgtcac cattccattg ggcagtgaca tcgatcactt tgccaacgtc
      721 gatccgcaaa aagttttgga actccgcaaa cagttgatcc cagacaaaga acgaacgacc
      781 cattcgatct tgctgtccgt cgggcgattg cgctactaca aaggcttgga cgatctgatc
      841 cgcgcgctgc ccgccatccc ggatgcgatc actgtgatag tcggcagcgg gccgatgcaa
      901 gctgaatggc ggcagctggt ccgatcgggg ggtgtagcca atcgggtcgt gtttgccggt
      961 gaagttgatg atcaagaatt gccgctctat tatgcagcta gtgatctctt tgtactgccc
     1021 gctaatgcgc gcgcggaagc cttcggcacg gtgatcgtcg aagccatggc ggccggccgc
     1081 gcggtgatct ccaccgaaat cggcacgggc acatcctgga tcaatgtgaa tggacagaca
     1141 ggtttagttg tcccaccgca cgcgcccgat caattggccg ctgcgataaa ctcattgttg
     1201 aacgatccag accggcgaaa gaagtacggt caagctgctc aagtccgcgc ccaagccgac
     1261 ttcagcgtaa ataaaatgat cgatcgggtt tacgacgtgt atcaatcact tatcgaacat
     1321 taatttttaa ggcatggcaa gctatggtat cattggcaaa cccacaagat gaaaaaccgt
     1381 cattgggcga acgtcagccg tccgatctca gagccaacgc aaagagattc atcgtttcgc
     1441 tcggaatgat atataggaat agtttctatg actaattcga gtcgtttgag tgcgctgtgc
     1501 gacaaaatga ttgaggccgg ttggatcgcg gcggtggtat tggtgccgct cttcttcaac
     1561 gtgtactcca gccgcgtgtt tgagccggac aaactgacgc tcttgcgatc gatcgccctg
     1621 acgatgattg cggcctgggc catcaagtgg atcgatcagc gcaagcagcc gaaggatccc
     1681 gatcgccccg atcggattac actccgcaca ccgctggtgc tggccacgat cattttagtc
     1741 gtcgtctatt taatctcgac tgttttttct ctcgtgccgg ccgttagttt ctttggctct
     1801 tatcagcggt tgcaaggcac ctacagcaca ttttcatata tcgtcgtctt tgcgatgatc
     1861 ctcgtccacc tgcgcaagcg cgaacaactc gatcgaatcg tgacggcgat tgtgatgacc
     1921 agcctgccca ttgcgttcta tggtttgctg cagcatggcg acggcaagaa ttcactcgat
     1981 ccgctgccgt ggggcggcga tgtgaccagc cgcgttgcct cgaacatggg caacgccatt
     2041 ttcgtcgccg catatttgat catggctttc ttcttgacgc tgggccgcgt agtcgagagt
     2101 ttccgcacga tccttaccga agaagaatcg cgcgtttcgg acattctgcg cgccagcgcg
     2161 tacatttttg tggccgccat tcaattgatc accttcggct ttgcggacag ccgcggcccg
     2221 ctcttgggct ggctgcccgg tatgttcatc ttcgggctcg tcagcttgtt gatgctgcgt
     2281 gtctcgctgc atagccagac cgaagaaaat gttgaggcaa ttgattcttc tccggcagcc
     2341 attcgttttc cgattaccgt gctcgatcta gttaaggcgg tcgtactgac tgccatcgca
     2401 gtgggcattg ccgccgccgc cgtgttcatc ttgctcaaat cgtctgaagc atccgaggtc
     2461 aaggtcaagc tcactctgat tgccgtcgtc ggcctgctcg gcggcctgct gccggtactg
     2521 gccgtcgtcg ggattcgccg caccgctgcg cgctgggctt ggatgtcgct gattttggtc
     2581 acgatttccg gcgcggttgg actgttattg atcaacttct ccaacattcc taaaattgtc
     2641 gagctccggc agagcggcgt gttcggtcaa ctcgcgacgt tgtttgaatc cgaaagcggc
     2701 accggcaaag tccgcacgtt gatctggcaa ggctcggagg cgctggtcac gccgcatgat
     2761 ccgatcaact ttcctgacgg tcacactgat ccgtttaatg tcattcgtcc gttgatcggt
     2821 tacggaccgg aatcaatgta tgtggcttac aacaatttct acccgtccga tctggctcac
     2881 tacgaatcgc gcaacgcctc gcccgatcga tcacacaatg agacctggga ctcgctggtc
     2941 atcaccggcg tcatcgggtt catcgctgaa cagttcctgt tcatcagcgt gtttttcttc
     3001 gcgttgaaat tcatcggctg gatacccaat cgcaaagcgg ccatgactta tatcgccttg
     3061 atgattgtgg gcggtattgg cggcgggctc ggcttagcct tgctcaaagg cgttaagttc
     3121 ttcggaccgg gctggccggg cggggtgacg gctggcatcg tagcttacgt gctgctgttt
     3181 gccttattcc acttccaaat ttcgcggcgg gtgtatctca tcatttcgct gatcttgatt
     3241 ttggtggttg aaggcgttat ctttggcgtg acatacaccg ccgagcagcg cacgatcgag
     3301 ttactgggcg gtgcaaccgg agccgccgct ttgatggggc tgttctacgt cattggacgc
     3361 tgggtcttta atgagaccgc cggtcaaccg atcgatatta gcagccatgt gtttatcatc
     3421 attgccttat tcggcgggat tctggcgcat tacatggaaa ttagttttgc cggaattgcg
     3481 atcgcggcca cgcgcacata cttctggacg ttcgccggat tattagtcgt caccggcttg
     3541 cactgggtgc cgacgtccga tgagccggct gcgccgatcg cgtctatgaa cgacgcgccc
     3601 agtgcaacgc tcgcgcaagc cagcccagcg atccaatcga acgtgccgcg ccacaagaag
     3661 aaatcgaaac gcgtcatcga gcaatcacgc gcggctcaat cgatcaagcg caactcgtcg
     3721 agcgcgctgc ccgcgtggct cgcgcccgtg atgacgatgg cggtgatcgg cacgttgatc
     3781 ttagtgacgc tgggctacga attcatcaac aacccgccgc gtgatccaaa ttcacccggc
     3841 tttaatccca atccgtcggc gacggaattg atcgtcaatt cgctgacgca cctgccttat
     3901 cagggcaaca aagaatcatc cggcgcgctg ttgatgttcg gggtgacgtg gttgttcggc
     3961 gggttattaa gcctggccga gctgcgccgg cgcggaacgt tggaagcaaa agatacgctg
     4021 atggccagtc tgagttattt cggtatctcg atcgggatga cactgctcgt ctggctgctg
     4081 ctggctggac aattattgag tctgggatcg atcgtccaca atacgcagat cacgacgctg
     4141 gccgatctca acgggcgcgt caacgaattg ttgacgctgg ccgacagtgt ggcgaacttg
     4201 ctgtcattct tctacttcgt cgtgttcgcc ttgatgatcg gcatggccgt gtcgatcatg
     4261 ctgcaaagcc gcacctggtt cggcaagtgg ggcactgatt ttggctggct gagtgcgatc
     4321 gtgattgtgc cgctgctgat catcggcatc aatacaacca acctgaacaa catccgggcc
     4381 gataccattt tcaaacaggc cgatccgctg aagggtcagg gtcaatggga cgctgccatc
     4441 gcgcattaca agaaagtgtc tagcttgcaa ccgaatgaag atttctatta tctgtggatg
     4501 ggtgcggctt ttctggaaaa agccaacacg gcgccgccgg cagcggccga tcgacccagt
     4561 atcatcacgc cgcaaacaac ttttaacagc atcatgaact tggatttcaa taagtcgtac
     4621 acactgaatc ggctcgatac gctgcaagtg gccttgaccg tgctgacgca cgcgcgagat
     4681 ctcaatccgc tcaacaccga tcattcggcc aacctggcgc ggctgtatcg gcgctggtct
     4741 gatctggaaa ccgataagca ggccaagcac gatcggttag tcacggccag cgattactat
     4801 cggcaggcca ccacgctcag cccgcaaaat gcgcagctct ggaatgagtg ggcgaccgtc
     4861 aatctcggcc tgcatgatgt cgatctccag ctgaacaacg atcaaggcgc ggctaaccta
     4921 cagacagcgc aagatctact ggatcattcg ttgacgctgg atagccaata cgatcagacg
     4981 tatatcattc gcgggcagct cgatcgcttc ttgggcaaga atgatgaggc aatccaagaa
     5041 tatcaaaacg cgctgaagtg gaatccggac tgcgccgatt gctggggcgg cctgtccgat
     5101 atattcagct cggcgggcaa ctacaccgcc gtcgagacga ttactttagc cttcatgcaa
     5161 actcacgccg attttgtgcc cggcctgcgc acactggcgc gcaatgccta catccccgcc
     5221 ggtcgattgg gcgaagcgat cgacacgcaa tcgcaagtgg tgtcattagc caatcaggat
     5281 ccaaatgtct gggacgatta tcgtgtgttg gcagtcatgt tggcacaagc cggtcgcttc
     5341 ccggaagcgc tgcaagccgc tcaaaccggc ctgaccaaag cgccgcagga caagcaaact
     5401 gagatgcaga gtctgatcga gcagctgcaa caacagctag gcgttgggcc gacactcacg
     5461 gacacggtgc ctgggccgaa cccaacccca acaccataac ttcagcatcg agagacgttc
     5521 cagttgaacg tctcactgag atcacattct atgacgactg ctatg
//
LOCUS       DASRTJ010000671         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_366813,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000671 DASRTJ010000000
VERSION     DASRTJ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366813"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..597
                     /locus_tag="VFF70_13570"
     CDS             472..597
                     /locus_tag="VFF70_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45763.1"
                     /translation="MAAIFHQARRLDQRIVRRPLLVSIVILIIFGGLLIRSGLAG"
     gene            735..>1810
                     /locus_tag="VFF70_13575"
     CDS             735..>1810
                     /locus_tag="VFF70_13575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45764.1"
                     /translation="MPPEPAAAAPRRFLKIKPNWNDLAFSALMPILAVLAALLVGAVM
                     MALLHVDPLQGYLELMGGAFGSVSGLTQSIVKATPLLLVGLGIVIAFRANVINIGGEG
                     QIILGALAGTWFALAFRDWPGWLLIPATMLSGFLAGGMWGFIPGFLKARLGVNEILST
                     VMMNAIALQLMNSLLRGPMMDPAELAKGTFLAQSERLPESVWLPRLVSQTQLHTGAIL
                     AIVLAVAVYIFLWRTTIGYRIRAVGLNPDAARYAGIRVPFYQALALTLAGGFAGLAGV
                     IEVIGVQHRLLQGITSGYGFTGIVAALFGGLHPLGSIPASFLFGGLLVGADKMQRAVQ
                     VPSALIDTLLGLIVLFVVGSGLLAR"
BASE COUNT          338 a          471 c          515 g          486 t
ORIGIN      
        1 gtgcggcatt gaccgctgcc acgccactgg ccggattggc gacaccaacc gcgcccgtca
       61 gtgcagtcgg actgcacgca ctcaaaacga gtgcggccga gacgatgatg gtcattagta
      121 cagtcttggc tttcattcta ctgcctccat gaattgctaa aaagttgttt gaactcaacc
      181 tcgatattaa cgccttgcca ttaaagcaac gtagcaataa gcttaatctt caatgaggat
      241 gatatttggt gtgggtcggc cgcgatgctc aaggcgttag cgtaaattaa gccagcctta
      301 atcaaatgct tctatggcag gacttacgct cagttgtcat ttcgagcgct tttttgcgag
      361 aaatctctac aatcataggc gagactcgtt catcagggca gagatttctc acttcgttcg
      421 aaatgacaag cgaaagttct gtatggatta ttcatcagcc tcttagtgat catggccgcg
      481 atctttcatc aagcccgacg gctcgatcaa cggatcgtgc gcaggccgtt gttggtcagc
      541 atcgtcattc taattatctt cggcggctta ttaatccgat cgggtctagc tggctgatcg
      601 aggcacttta cgttattttt tgcgtgaaat cacgcccgcg cgggcctggc tcagcgccgg
      661 gtatatgatg ttagacctaa taagcaaggt caactgtcat ttgctttatg cttccatttc
      721 actatactaa tgaaatgccg cccgaacccg cagcagctgc tccccgtcga tttctaaaaa
      781 ttaagcccaa ttggaatgat ctcgccttta gcgctttgat gccgatcttg gccgtgttgg
      841 cggcgctgct cgtcggtgcg gtgatgatgg cgctgctcca cgtcgatccg cttcagggct
      901 atctcgaact catgggcggc gcctttggca gtgtctccgg tctcactcaa tcgatcgtga
      961 aagctacgcc gctgttgctg gtcggtttag gcatcgtcat tgcgtttcga gcgaacgtga
     1021 tcaacatcgg cggcgaagga cagatcatcc tcggcgcact ggcaggcacc tggttcgcgc
     1081 tcgcctttcg tgactggccc ggttggctgt tgattccggc gacgatgctg tccggttttt
     1141 tggccggagg aatgtggggc ttcattccgg gttttctcaa agcccgtttg ggtgtgaatg
     1201 agattctcag cacggtgatg atgaacgcca ttgcgctgca gttgatgaac tctctgctgc
     1261 gcggaccgat gatggatccg gccgaattgg ccaagggcac tttcctggcg cagtccgagc
     1321 gcttgcctga aagtgtgtgg ctgcctcgcc tggtttcaca gactcaactg catacgggcg
     1381 cgatcttggc gatcgtgctg gcggtggccg tttacatttt cctgtggcgc accacgatcg
     1441 gttatcgcat ccgcgccgtc ggcttgaatc cggatgcagc ccgatatgcg ggcattcggg
     1501 tcccgttcta tcaggcttta gcgttgacat tagcaggtgg tttcgccgga ctggccggtg
     1561 taatcgaagt gataggtgtg cagcaccgat tgcttcaagg gatcaccagc ggttacggtt
     1621 tcactggaat cgtcgcggcg ttgttcggcg gtttgcaccc gctggggtcg atcccggctt
     1681 catttttgtt tggcggcttg ctcgtcggcg cggataagat gcagcgcgcg gtgcaagtgc
     1741 cgtcggcgct gatcgatacc ttgctaggtc tgattgtgtt gttcgtcgtc ggatcgggtc
     1801 tgctggcacg
//
LOCUS       DASRTJ010000672         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_210925,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000672 DASRTJ010000000
VERSION     DASRTJ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210925"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..>1606
                     /locus_tag="VFF70_13580"
     CDS             320..>1606
                     /locus_tag="VFF70_13580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZY45765.1"
                     /translation="MNLSRLIAALPSIETQPQSDPDITLITADSRQVIPGALFVAYPG
                     VSIDGHDFIHDAITRGAVAVIGELDLNELPIPQSTIRIPNYIRVRSGREAFAYLHAAW
                     NDYPARKLIMIGVTGTDGKTTTTNLIHSILTAAGIKAGMISTVNALIGDRALDTGLHT
                     TTPDADEVQLYLKEMVDAGMTHCVLETTSHGLMQHRVDGCEYDIAVVTNITHEHLDMH
                     GSYEAYRAAKSILFESLASSFDKGLPKSAILNLDDSSYPYLKNAIERSADRGARHANI
                     LTYSVKHPANCAAIEIDYRPDAIHFTVIGTGYSIDISTSLVGEYNVSNCLAAIAATID
                     GLQIDPQIVRRGIANLKGVPGRMERINAGQDFIAIVDFAHTPNALDQAIAAARTMTDG
                     QVIVAFGCAGLRDRDKRRLMGEVVGKSADKIVITAED"
BASE COUNT          388 a          474 c          407 g          337 t
ORIGIN      
        1 gtccggaccg atgaagcgaa acgaaaagaa taggattgct gtggctaaca gcagccagat
       61 gaatacgcgc gatcgagttg aaggtgagag cgattcgagg ggatgggaca tatggctcct
      121 ttattaacgg ggcaagttaa taacttgtcc tgcattatac tttcaatcgc ctcacaatat
      181 gaaatgcaaa cggcgtcatc gaatagacaa caaatcagcg aatgacgtcg attcaatcat
      241 tcgctgattc gatgcagatt cgctgacacc ctcagccttc atccttctgc ctgacagttt
      301 ctttggtaca atccatcgca tgaatctcag ccgcttgatc gctgcactcc cctcgatcga
      361 aactcaacca caatctgatc ccgacatcac cttgatcact gccgattcgc gccaagtgat
      421 tccgggcgcg ctgttcgtcg cctatcccgg cgtatcgatc gacgggcatg acttcattca
      481 tgacgccatt actcgcggcg cagttgctgt tattggagaa ctcgatctca acgaacttcc
      541 cattccgcaa tccacaatcc gcattccgaa ttacattcgc gtccgcagcg gccgcgaagc
      601 cttcgcctat ctgcacgccg cctggaacga ttatcctgcg cgcaagctga tcatgatcgg
      661 cgtgacgggc acggacggca aaacgacgac aaccaatctc attcactcca ttctgactgc
      721 cgccggaatc aaggcgggga tgatcagcac ggtcaacgcg ctgatcggcg atcgagcgtt
      781 ggacaccggc ctgcacacga ccacacccga cgccgacgaa gtgcagcttt acctcaaaga
      841 gatggtcgat gcgggcatga cgcattgcgt tttagaaacc acttcgcacg gtttgatgca
      901 gcatcgcgtc gatggctgcg agtacgacat cgccgtcgtc accaacatca cgcacgaaca
      961 tctcgacatg catggctcgt atgaagccta tcgcgccgcc aagtcgatcc tctttgaatc
     1021 gttagcctct tcatttgata aaggtctgcc aaagtcggcc atcttgaatc tcgacgattc
     1081 atcctatccg tatttgaaaa atgcgatcga gcgatcagcc gatcgaggcg cacgccacgc
     1141 caacatcctg acttacagcg tgaagcatcc ggccaactgc gccgcgattg agatcgatta
     1201 ccggccggat gcgatccact tcacagtgat cggcacgggt tactcgatcg atatttcgac
     1261 ctcattggtc ggcgaataca acgtctccaa ttgcctggcc gcgatcgcag ccacgatcga
     1321 tggcctccag atcgatccac aaattgtccg gcgcggcatc gcgaatctca aaggcgtgcc
     1381 gggccgcatg gaaagaatca acgcggggca ggacttcatc gccatcgtcg attttgcgca
     1441 cacacccaac gcgctcgatc aagccatcgc cgccgcgcgc acgatgacgg acggacaagt
     1501 gatcgtcgcg ttcggctgcg caggcctgcg cgatcgagac aagcgcagac tgatgggcga
     1561 agtggtgggg aaatctgcgg ataaaattgt gatcacggct gaagac
//
LOCUS       DASRTJ010000673        11946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_210936,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000673 DASRTJ010000000
VERSION     DASRTJ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210936"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFF70_13585"
     CDS             complement(<1..250)
                     /locus_tag="VFF70_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45766.1"
                     /translation="MKTFSGFASSSSKQVSFPDQFFTELLPLIDDLVELKVTLACFWL
                     FDQKTGLLKWATVKGLESDPALKDIQANINVGLEKAVER"
     gene            complement(284..1627)
                     /gene="dnaB"
                     /locus_tag="VFF70_13590"
     CDS             complement(284..1627)
                     /gene="dnaB"
                     /locus_tag="VFF70_13590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZY45767.1"
                     /translation="MTTTPIERMPPHSIEAEEAVLGSILIDPEAIFRVSPFLKTEDFY
                     IVKNQWMWDVCLALHERREPIDFVTVTRELEARNQISEIGGAAYVSQLINVVPTAIHA
                     EGYGRIVERSALRRRLLNTASKIAQLAYEESEDIDATIDAAEQSLFAVSQRRISRDLL
                     PISDVIRSYYDRIEYLYEHRGEPLGIPTGFVDLDKLLGGLQKSDLIILAARPGVGKTS
                     LMLNIGLNAARRFHQRVGVFTLEMSNEQVVQRLVSAETGIDSQRLRLGELHEDEWPLF
                     VQATGALSDALMFIDDTPSISALQLRTKARRLYAEHGLDLIIIDYLQLMSSDQRTENR
                     VQEISYLSRALKGLARELNIPVLVASQLSRAVEQRSDKRPILSDLRESGSIEQDADIV
                     MFIYREDMYDENSDRKNIAELIIAKHRNGPTGTIELFFQKNLTMFRNALKRDIAL"
     gene            complement(1772..2272)
                     /locus_tag="VFF70_13595"
     CDS             complement(1772..2272)
                     /locus_tag="VFF70_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZY45768.1"
                     /translation="MIEVSIDSIRVSLMSQHRVVILKDAETERYLPIWIGACEADAIT
                     IELQGVEVARPFTHDLLKSAITELGGKITQVLISDLRNEVFYAQIVLDVSGRKLEIDS
                     RPSDALALAVRAKVPIYIDESVMDKASITPEEDIAEGSGDEPLTRSPFKDLIEGLDLD
                     DFGEEK"
     gene            2507..3304
                     /locus_tag="VFF70_13600"
     CDS             2507..3304
                     /locus_tag="VFF70_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45769.1"
                     /translation="MSQFAPWIILVGALIFALSLWIVIRASNQARQGAFYFMRTDALQ
                     RARRWLLMGTAILILSISVGVILSNSPQPVAIANVTPLTATRIISPTKTSTPSPMPTS
                     TSTSTASPTPVISSTVTPTIKPGSVPNLLLTPLPSAVPVAPGAKFTLTTLASVLDSKQ
                     SPVDGGTVFPQGTRAIHIFFRTSGVNNAARWAVFCTKGNQLVDSFIDLWKWGPIAQGA
                     HAVCSIDGSIGAYTVTSYLDFNKQFVAPFTIIVPPTATATPAPTTAP"
     gene            complement(3360..4829)
                     /locus_tag="VFF70_13605"
     CDS             complement(3360..4829)
                     /locus_tag="VFF70_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45770.1"
                     /translation="MSIRLRLTLWYSFVLAALLIALGVAIYTILSNNLLSNIDTQLNS
                     TADEILGASHIRSFSNILQVVIPDELDIFRASGVGVIVIDRSQTIVNKSSNVGNLKQT
                     FDPDVLPLITLDNRLTREVIVATAHLRVLTVPINIGSDQVGYLQVAASLSSVDQSLQQ
                     LGAIMLLSGIFGVLAAAIVGAFLASQALRPIDQITQTALSIARAGKLDQRIPATMTHD
                     EVGRLSETFNLMLDRLEGLFGSQQRFIADVSHELRTPLTTIRGNIDLLKRIGGADPAS
                     LDAIRGESDRMIRLVGDLLLLAQADGGLPLRREAVDLDRIAAEVVQQVQVIAGKVRVT
                     LHSPNEEEPTGVTGDADRLRQLVLNLVDNAIKYTPAGGEVVVTVDHSDGWARLDVCDT
                     GPGIPPEHLQLSPNGVPLIFERFYRVDKSRVRSGVKSRSGGGTGLGLSIAYWIVQMHA
                     GRIEVHSEIETGTTFRVWLPRRSAMLPVTNPPQLIEGAQ"
     gene            complement(5008..5685)
                     /locus_tag="VFF70_13610"
     CDS             complement(5008..5685)
                     /locus_tag="VFF70_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45771.1"
                     /translation="MADKILVVEDDEGISGTLFRGLTFEGYKVLTAADGPGGLAIARD
                     DPPDLVVLDWMLPGLDGLEVCKRLRAAGSTPILMLTAKDAIADRVQGLDAGADDYLVK
                     PFAFDELLARVRALLRRSKPVEGSEVLKFADMVLDTGAREVRRGPHKIDLTAKEFDLL
                     ELFIRHPKQVLTRDMIYDRIWGYDFGGESNIIEVYVRYLRQKTEVEGSGRLLQTVRGI
                     GYVLREE"
     gene            complement(5776..6312)
                     /locus_tag="VFF70_13615"
     CDS             complement(5776..6312)
                     /locus_tag="VFF70_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HZY45772.1"
                     /translation="MGRTLLLTGRPGIGKTTLIKAVVEQLKGRVGGFYTEEILGPGGR
                     KGFRLTTLEGRSQVVAHIDHKSRSTVGRYGVDVAAIDRVGAKVIMQALDRYPIILIDE
                     IGRMQLFSSQFQSAVLKAVSSPKLVVATLLLEDHPWTGALKALPFVTTWEVTLKNRSK
                     LVAEILNWLEAQSVMRDS"
     gene            6457..7653
                     /locus_tag="VFF70_13620"
     CDS             6457..7653
                     /locus_tag="VFF70_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZY45773.1"
                     /translation="MDQDRSDTRTLVVLLTIAVSIYLIEKLGQALLSLSNVLLFLALA
                     WLIAFSLQPLVKFIHQLSAPPRVNDWQRKHWGDKWADRLAHPSLGFSILIVYTLLVAG
                     IVIIILAIVPLIVEQVRQLAVTVQQQATNLPESIQRVSDWLNSVRDFLITRLQLDPSQ
                     IPLPAPQEFIGQLASQLAAFGTSLVQFGLALATGIAQFLLVVFLSVMVMIDGGRLAQQ
                     LMLSIPKRYQPNVQFAINTLQRTFGGFLRGTLLQGIIYGLGVIILMTILGIGSAIATG
                     VATGILMLIPIVGGPIGLFIPLLVGLLQGSPNTFWLILVLVVYQVALFNFITPRLLSK
                     SLRMPSLLVILSLLIGAQLLGIWGFIFAVPISAAIYTIGLLVLGETKRRQDASDAARE
                     REKALP"
     gene            7658..8428
                     /locus_tag="VFF70_13625"
     CDS             7658..8428
                     /locus_tag="VFF70_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZY45774.1"
                     /translation="MSNFKVRGATPDEVQPFAFDRGPIGCVLLHGFTAAPKEMRPLGE
                     YLADRNYTIRGVRFAGHATSPEDLAQTTWRDWVASAKEAVTELRQRCQQVWIIGLSLG
                     GLIGLHLAEHHLVNGVVAMAPPILTPDRRMPFARFLWRFKPYSIKSLANLHDEAALLQ
                     HADYEHNPTRAIAEMYALIRRTRADLSMIDCPLLLIFARHDRVVSIANADYVWSRVRS
                     THKEKLILDRGGHIITEDLDKAVAFQKIEAFLAEFTRK"
     gene            8624..9514
                     /locus_tag="VFF70_13630"
     CDS             8624..9514
                     /locus_tag="VFF70_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZY45775.1"
                     /translation="MTASKRLLFVFPHPDDETFATGGSIAKYAHEGVEVHYACGTRGE
                     VGTVDAERLKPYEHLPEDQRVGALRGQELRCAADKLGLTGLHYLGYRDSGMTGSPEAQ
                     DPRALINANADEVTLKLVELIRSIKPHVIVTHDPFGGYGHPDHIFLHHQTTAAFQAAG
                     DPNKYPDAGAPYQPQKLYWHVFPKALFNFLIKASRLLRPKGNKFGRNNDIDIDEIAKH
                     DYPPTTRIDIWPYYSIKQEASQCHVSQLAGSGGGMLDRIPRLFRRRLLGYEHYRLVEP
                     EPNGRRRKEQDLFEDLEIRS"
     gene            9600..10382
                     /locus_tag="VFF70_13635"
     CDS             9600..10382
                     /locus_tag="VFF70_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45776.1"
                     /translation="MKKYISGLIILAVFFGLSVSPTGAAALSGSVDIFTTAVCKDTAT
                     VAVSGSSDWATNRVKVSFYKQDSNGNYNWFANATSDTFGSGSFVVPVVADYSTKAIPE
                     GTSLRIDVELQHQSGSGGGFYTAASTSTYLTAADKLCFNKCSVTITSRDKAPASGTIT
                     LRSHFGSWFRPEGWLHAAIPVNANQFVKTTIVGVPCDWTVRAWYYPSTGSNRTPKLLP
                     SQYWPGDFAASTSDGALPYAAAFANGLVATKPLESDDPYAPK"
     gene            10417..10698
                     /locus_tag="VFF70_13640"
     CDS             10417..10698
                     /locus_tag="VFF70_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZY45777.1"
                     /translation="MYARSRAFCFQLGHDQTLTIFEWFFNEPGAGVGWESVQQTIAFS
                     DEIQQEDIEICETVQHGLQSRTYHRGRFSVKGENGVHHFQPLLYEFVTK"
     gene            complement(10737..11444)
                     /locus_tag="VFF70_13645"
     CDS             complement(10737..11444)
                     /locus_tag="VFF70_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZY45778.1"
                     /translation="MSSIETFLNTYGLIAIFAVMLIKAIGVPIPIPADVIMLATSARV
                     VEGKFILWQAFVSVLIALLIGGLIQFLIVRGPGRKFLYRFGRYLGLTATRLDAAASIV
                     KQRNPIGLGLIILTPGVRAISVPACGIADVPIGKFTVGLLIGEGLFLSLHFFLGAIIG
                     SLVSQFAQVIPLPILIVAISVLVLLSLGMWIIIRRRQRPALSRRQLVAESFEVWHSAT
                     CPVCLTLGAVSRVNSQV"
     gene            complement(11454..>11946)
                     /locus_tag="VFF70_13650"
     CDS             complement(11454..>11946)
                     /locus_tag="VFF70_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45779.1"
                     /translation="YGLYALNAFWALFGTVALYYRLRSVKEGWAFFATLVGLAASIGS
                     IVGSLYLIGHLHYVAVAFATNPEFARAEFLLPSPVNPFGVMTFGLTSVWFLIAALLML
                     RSNFPKLLGYLGLIAFADLAVGFIGSILGMDLVATIASLIAGAVGGPIFWLWLGVILR
                     REA"
BASE COUNT         2790 a         3465 c         3069 g         2622 t
ORIGIN      
        1 cgcgctcgac ggctttctca agtccaacat tgatgttcgc ctgaatatcc ttcaacgctg
       61 gatcgctttc caaacctttt actgtcgccc acttcaacaa tccggttttt tgatcaaata
      121 accaaaaaca ggctagcgtg actttcaatt caaccagatc atcgatcagc ggcagcagct
      181 cggtgaagaa ctgatccggg aatgaaactt gtttgcttga actgctggca aagccgctga
      241 aagttttcat gtttacattg taggggcgag cggccgctcg cccctacaag gctatatccc
      301 gcttcaacgc attgcggaac atggtcaaat tcttctggaa gaacaattcg atcgtgccgg
      361 tcgggccgtt gcggtgcttg gcgatgatca gttcggcgat gttcttgcga tccgagtttt
      421 catcatacat gtcttcacga tagatgaaca tgacaatatc ggcgtcctgc tcgatcgaac
      481 cggattcgcg caagtcgctc aagattgggc gcttatcgct gcgctgctcg acggcgcggc
      541 ttaactgcga agccaccaag actggaatgt tcaactcgcg cgccagacct ttcaaggcac
      601 gcgacaaata gctgatctcc tgcacgcgat tttcggtgcg ctgatcgctg gacatcaatt
      661 gcaaataatc gatgatgatc aaatcgaggc cgtgctcggc atacaaacgc cgggctttgg
      721 tgcgcaattg cagcgcagaa atactgggcg tgtcgtcgat gaacatcagc gcatcgctca
      781 acgccccggt agcttgcaca aacagcggcc attcatcttc gtgcagttca cccaagcgca
      841 agcgctgact gtcgatgccg gtctcggcgc tgaccagccg ctgcacgact tgttcgttgg
      901 acatctccag tgtgaagaca ccaacgcgct gatgaaaacg ccgcgccgca ttcaagccga
      961 tattcaacat caaacttgtt ttgcccacgc cgggccgcgc cgccagaatg atcaggtcgg
     1021 atttctgtag gccgcccaac aatttgtcaa gatcgacgaa gccggtcggg atgccgagtg
     1081 gttcgccgcg atgctcatac agatattcga tgcgatcata gtacgatcgg attacgtcgc
     1141 tgatcggcag cagatcgcgc gagatgcggc gctggctgac ggcgaataac gactgctcgg
     1201 cggcatcgat cgtcgcgtcg atgtcttccg actcttcata ggctagctgc gcaattttac
     1261 tggcggtgtt gagcagccgc cggcgcagcg ccgatcgttc gacgatgcgg ccataacctt
     1321 cggcatggat ggccgtgggc acaacgttga tcagctgcga cacatacgcc gcaccgccga
     1381 tctcggagat ctgattgcgc gcttccaatt cacgcgtgac cgtcacaaaa tcgatcggtt
     1441 cgcgccgctc gtgcagcgcc aggcacacat cccacatcca ctgattcttg acgatgtaga
     1501 aatcttcggt cttgaggaac gggctgacgc ggaagatcgc ttcgggatcg atcaagatcg
     1561 atcccaacac agcttcttcg gcttcaatgg agtgcggcgg catccgttcg atgggcgtgg
     1621 tggtcatacg cagtgaactc tcaatataat tgatgattga ataaatagcc cgcgttgttt
     1681 agaggttttt tccaagcacg cgggcagatc tatcaataat ttatccgctt aggcgcggat
     1741 gtcaggcagc taaaccgatc gggttagccg atcacttctc ttcaccaaag tcgtccagat
     1801 ccaagccttc gatcaaatct ttgaacggcg aacgggtcag cggctcgtca ccactccctt
     1861 cggcgatatc ttcttccggc gtgatggagg ctttatccat gacggattca tcgatgtaga
     1921 tcggaacttt ggcgcgtacc gccaaggcta aggcatcgct ggggcgcgaa tcaatctcta
     1981 atttgcggcc gctgacatcc agcacaatct gggcgtagaa aacttcgttg cgcagatcgc
     2041 tgatcaacac ttgcgtaatc ttgccgccga gttcggtgat ggcggacttg agcagatcat
     2101 gcgtgaaggg ccgcgccact tccacgcctt gcaattcaat cgtaatcgca tccgcttcgc
     2161 aggcgccgat ccagatcggc aggtagcgct cagtctcggc gtctttcaaa atgacgacgc
     2221 gatgctgcga cattaaactc acccgaatac tatcaatgct gacttcaatc ataggctgac
     2281 gcctcccgct caacacaccc cagcatgtct gcgattatac cacacgcgtt gagcggccag
     2341 aacgatcgga tgcacaccaa cccaatcgat tttcatcaat ttgacacatg gtataatcgt
     2401 ttttagccaa tggtcatgac gatcgctaaa acaatcgctg agattatcac agtcaactcc
     2461 gatcgaaccg ttgttaacgg agccacactt ttcaggataa gtcgcattgt cacaatttgc
     2521 accatggatc atcctcgtcg gcgcattgat ctttgccctg tcgctatgga tcgtcatccg
     2581 cgccagcaat caagcgagac aaggcgcatt ttatttcatg cgcacagacg cgttgcagcg
     2641 tgcccgccgc tggttgctca tggggaccgc cattttgatc ctcagcatca gcgtgggcgt
     2701 catcctatcg aattcacccc aacctgtagc cattgcgaac gttacaccgt tgactgccac
     2761 gcggatcatt tcaccgacaa aaacaagtac gcccagtcca atgccaactt caaccagcac
     2821 gtcgacagca tcgccgacgc ctgtcatttc cagtacggtc acgccgacca ttaagcccgg
     2881 ctcagtaccc aatttattgt tgacgccgct accttcggct gtgccggttg cgcctggggc
     2941 aaagtttacg ttgacgacgc tcgcttctgt gctggatagt aagcaaagcc cggtcgatgg
     3001 cggcaccgtg tttccgcaag gcacacgcgc tattcacatt ttcttccgca ccagcggcgt
     3061 gaacaacgct gcgcgttggg cggtgttctg caccaaaggc aatcaactgg tagacagttt
     3121 cattgatctg tggaagtggg ggccgatcgc gcaaggcgca cacgcggtct gcagcattga
     3181 tggatcgatc ggcgcttata cagtgacgag ctatctcgat ttcaacaaac agttcgttgc
     3241 gccctttacg atcattgtgc cgcccacagc gacggcgaca cctgccccaa cgactgcgcc
     3301 gtaaatcaaa tcagcgcctg aaataaaaag agcgcagtcg tgtagactgg cgctcttttt
     3361 tattgcgcac cctcgatcaa ctgcggcgga ttcgtcaccg gaagcatagc cgatcggcgg
     3421 ggcagccaca ctctaaacgt tgtgccggtc tcgatctcgc tgtgcacttc gatgcggccc
     3481 gcatgcattt gaacgatcca ataggcgatc gataacccca gacccgtgcc gccgcctgat
     3541 cggcttttca cgcccgatcg gacgcgtgat ttgtcgacgc gatagaagcg ttcgaaaatc
     3601 aacggcacac cattcggact gagctgcaaa tgttcaggcg gaatgccagg gcctgtgtcg
     3661 caaacatcca agcgcgccca accatcagag tgatccaccg taacgaccac ttcgccacca
     3721 gcgggtgtgt acttgatcgc gttgtcgacc aaattcaaaa cgagttggcg taaccgatcg
     3781 gcatcgccgg ttacgcccgt cggctcttct tcattcggcg aatgtaaagt cacgcgcact
     3841 ttgccggcaa tcacttgaac ttgctgaact acttctgccg cgatgcgatc cagatcgacc
     3901 gcctcgcgcc gcagcggcaa gccgccatca gcctgcgcca acaacaagag atcaccgacg
     3961 agccggatca tccgatcgga ttcgccgcgg atcgcatcca atgaggccgg gtctgcgccg
     4021 ccgatgcgct tcaagagatc gatgttgccg cgaatcgtcg tcaagggcgt ccgcagctca
     4081 tgcgacacat cggcgatgaa acgctgctgc gatccgaata agccttccaa tcgatcgagc
     4141 atcaagttga atgtttcact caaccgaccg acttcgtcat gtgtcatggt ggcgggaatt
     4201 cgttgatcca acttgccggc acgggcgatc gataaggcgg tctgcgtgat ttgatcgatc
     4261 ggccgcaggg cctggctcgc cagaaacgcg ccgacgatcg ctgccgccaa aacgccgaag
     4321 atgccgctga gcagcataat cgcgccgagc tgttgcagcg attgatcgac actactcaac
     4381 gaagcggcca cttgcaagta acccacttga tcgctgccaa tgttgatcgg gactgtcagc
     4441 acacgcaggt gcgcagtcgc gacgatcact tcacgcgtta agcgattgtc caaagtaata
     4501 agcggcagaa catccggatc aaacgtttgc ttgaggttgc cgacattgct ggacttgttg
     4561 acaatggttt ggctgcgatc gatcacaatc acacccacgc ctgaggcgcg gaagatatcc
     4621 agctcgtcgg gaatgacgac ttgcaagatg ttcgagaatg atcggatatg gctggcgccg
     4681 aggatctcgt cggccgtgct gttgagctgc gtgtcgatat tggacagcag gttgttcgat
     4741 aaaatggtat agatcgccac acccaaagcg atcaagaggg ccgcgaggac aaaggaatac
     4801 cacaaggtga ggcgtaaacg aatagacatg atctcaacga caaataagca aatgacaaat
     4861 gcagaacaga caataagagt ctatctgtgt tctaaggatg aagcaatcgg cttaataggg
     4921 gatgaaggag aagtgagaat acaggctgtt aaggcgccga ttcgcgatga taaattcttt
     4981 gcgcctgagc ggctgtgcgg gtgatcatta ttcttcgcgc aagacatagc caatgccgcg
     5041 cacggtctgc agcagacgac cgctgccttc cacttcggtc ttttgacgca aatagcgaac
     5101 gtaaacttca atgatgttgc tctcgccgcc gaagtcatag ccccaaatcc gatcgtagat
     5161 catatcgcgg gtcagcactt gtttgggatg gcgtataaac aattccagca gatcgaattc
     5221 cttagcggtc agatcgatct tgtgcgggcc acgccgcact tcgcgcgcac cggtatccag
     5281 caccatgtcc gcaaatttca aaacctcgct gccttctacc ggtttgctgc ggcgcagcag
     5341 cgcacggaca cgcgccaaca attcgtcgaa ggcgaatggc ttaaccagat aatcgtcggc
     5401 accggcatcc agcccctgca ctcgatcggc gatcgcatct tttgcggtca gcatcaagat
     5461 cggcgtcgag cccgccgccc gcaatcgctt gcagacttcc aacccgtcta ggccgggcag
     5521 catccaatct aagaccacca gatcgggcgg gtcatcgcgc gcaatcgcca aaccgccggg
     5581 gccatcggcc gcagtcagga ctttgtagcc ttcaaaagtc agaccacgaa acaaagtgcc
     5641 ggaaattcct tcgtcgtctt ccaccaccaa aattttgtcg gccataggtt aattatattc
     5701 gcgccctgca gaatatgcaa aacttgaaaa atgatccgta acgagtgaca cgcgaacgcc
     5761 tattatcatc acgtatcacg aatcacgcat cacactttgc gcctcgagcc aatttaaaat
     5821 ttccgccacc aacttcgatc gatttttgag ggtgacttcc caggtcgtga cgaacggcag
     5881 cgccttcaat gcacctgtcc acggatgatc ttccaacagc agcgtggcca cgaccaactt
     5941 cggactgctg acggctttca acacggcgct ttgaaactgc gatgaaaaca gctgcatccg
     6001 gccgatttcg tcgatcagga taatcggata acggtccaat gcctgcatga tgaccttcgc
     6061 cccaacccga tcgatcgcgg cgacatctac gccatagcgc cccacggtcg atcgactttt
     6121 gtgatcgatg tgcgcgacta cctgcgatcg accctccagc gtcgtcaagc gaaagccttt
     6181 gcgtccgccc gggccgagaa tctcttcagt ataaaatcca ccgacccggc ctttcagttg
     6241 ctcaacgacc gctttgatca aagtcgtttt gccaatgcct ggccggcctg tcaacaataa
     6301 tgtgcgaccc atatgaattt tttgatctct gattgattgc caaggccagt ataacacact
     6361 gattattgat catttgttca ctgctcattt gtcattgtcc gcgtgtcatt gtacaatcgc
     6421 aacattccgc attcggcatt taccctggga gaccgcgtgg atcaagatcg atctgacact
     6481 cgaacactcg tcgtcctgct gaccattgcc gtttcgatct acctgatcga aaaattaggt
     6541 caagcgttgt tgagcttgag caacgtacta ttgttcctgg cgctggcctg gttgatcgcg
     6601 ttcagcctgc aaccgctggt caaattcatt catcaactct ctgcgccgcc gcgcgtcaac
     6661 gattggcagc gcaaacactg gggggacaag tgggccgatc ggttggcgca tccctcgctc
     6721 ggcttttcga tcctcattgt ctacacgctg ctggtcgccg gcatcgtcat tatcattctc
     6781 gcgattgtgc cattgatcgt cgagcaagtt cgccagctgg cggtcaccgt tcaacagcag
     6841 gccactaatt tgccggagag tattcagcgc gtcagcgatt ggcttaactc ggtgcgcgat
     6901 tttctgatca ctcgactgca gctcgatccc tctcaaattc cactgcccgc accgcaagaa
     6961 tttatcggcc agctggcctc acaattagcg gccttcggca ccagcttagt gcagttcggc
     7021 ttggctttgg cgaccggcat cgcccaattt ctactcgtcg tcttcctcag cgtcatggtc
     7081 atgatcgatg gcggacgatt ggcacagcag ctcatgctgt ccattcccaa gcgctatcaa
     7141 cccaacgtgc agtttgccat taacaccctg cagcgcacct ttggcggatt tttacgcggt
     7201 accttactgc aaggcatcat ctacggcttg ggcgtgatca tcttgatgac gatcctgggt
     7261 atcggctcgg cgattgcgac cggcgtcgcg accggcatct tgatgctgat cccaatcgtc
     7321 ggcggcccga tcggtttatt catcccgctg ctcgtgggac tgctgcaagg ctcacccaat
     7381 accttctggc tgattctggt gctggtcgtt tatcaagttg ccctgttcaa cttcatcacc
     7441 ccgcgcctgt taagcaaatc actgcgcatg ccgtcgctgc tggtgatctt gtcgctgctg
     7501 attggcgcgc agctgctggg catctggggc ttcatcttcg ccgtgccgat cagcgccgcg
     7561 atctacacca tcggcctcct cgtgttgggc gaaaccaaac gccgccagga tgcttcggac
     7621 gccgcccgcg aacgagaaaa agcgctgcct tgaaacgatg tcgaacttta aagtacgcgg
     7681 tgccacgccc gatgaagtgc agccgtttgc atttgatcgc ggcccgatcg ggtgtgtgct
     7741 gctgcacggc ttcactgccg cgcccaaaga aatgcggcca ctgggcgaat atctagccga
     7801 tcgaaactac accattcgcg gtgtgcgttt cgcaggccac gccacctcgc cggaagattt
     7861 ggcgcaaacg acttggcgcg attgggtcgc ctcggccaaa gaagcggtga ccgaattgcg
     7921 gcaacgctgc cagcaagtgt ggatcatcgg cttgtcgctc ggcggcttga tcggcttgca
     7981 tctggccgag catcacttgg tcaatggcgt ggtagcgatg gcgccgccta tcctcacgcc
     8041 cgatcgacgc atgccctttg cgcgtttctt gtggcgcttc aagccatatt cgatcaagag
     8101 cctggctaac ttgcacgatg aagccgcttt gctgcaacac gccgattacg aacacaatcc
     8161 cactcgcgcg atagcagaaa tgtatgccct cattcgccgt acccgcgccg atctcagtat
     8221 gatcgactgc ccactgctgc tgatcttcgc ccggcacgat cgcgtcgtct cgatcgcgaa
     8281 tgccgactat gtctggtcac gcgtgcgctc gacgcacaaa gaaaaactga tcctcgatcg
     8341 cggcggccat atcatcaccg aagaccttga caaagctgtg gcgtttcaaa agattgaagc
     8401 attcttagca gagtttactc gcaaatgata acggaggcgt gttccgtttg attttacgca
     8461 ccacgcatca cgattcactc atcactccta taggtgacgg aatcctcgta agcacccgac
     8521 ctgcaagctt ctcaatccgt gttacaattt tcttcgatga ttgatcggcg cagtacagcg
     8581 gcggatcggc cgccaacttt caagacagga taacttcaag cacatgacgg cctcaaaaag
     8641 attattgttt gtatttccac accccgatga tgaaacgttc gccaccggcg gatcgatcgc
     8701 caagtatgcc cacgagggtg ttgaggtgca ttacgcctgc ggcactcgcg gcgaagtcgg
     8761 cacggtcgat gccgagcgct tgaaacccta tgaacatttg cctgaagatc aacgcgtcgg
     8821 cgcattacgc ggacaggaat tacgttgcgc ggcagacaaa ttgggtttga ccggcttgca
     8881 ctatttaggc tatcgcgatt ctggcatgac cggttcgccg gaggctcaag atccgcgcgc
     8941 gctgatcaac gcgaatgccg atgaagtcac cctcaaactc gtcgagctaa tccgatcgat
     9001 caagccgcac gtcatcgtga cgcatgatcc tttcggcggc tacggacatc ccgatcatat
     9061 tttcttgcac catcaaacga cggcggcgtt tcaggcggcg ggcgatccaa acaaatatcc
     9121 ggatgcgggc gcgccgtatc agccgcaaaa attatattgg cacgttttcc cgaaagcgct
     9181 cttcaacttt ttgatcaaag cctctcgcct tctccgtccg aaaggaaata aatttggccg
     9241 caacaacgac atcgacattg atgagatcgc caagcacgat tatccgccca cgacccgcat
     9301 cgatatttgg ccctactatt cgatcaagca agaggcctcg caatgccatg tcagtcaatt
     9361 ggccggcagc ggcggcggaa tgctcgatcg aattccacgc ctgtttcgtc gaaggttgtt
     9421 aggttacgag cactatcggt tggtggaacc ggagccaaat gggcggcggc gtaaagaaca
     9481 agacttgttt gaggatttag aaattagaag ttagacatta gcgattaaat gtcagttcac
     9541 ccatcgtcac ttcaccttct catatccgat atccaagttc caaaacacag gagttaccaa
     9601 tgaaaaaata tatctcaggg ttaatcatct tagccgtgtt cttcgggttg tcggtcagcc
     9661 cgactggcgc agcggcgctc agcggcagcg tcgatatttt cacaaccgcg gtgtgcaaag
     9721 atacggcgac ggtcgccgtg tccggttcat ccgattgggc gacgaaccgg gtgaaggtca
     9781 gcttctacaa acaagacagt aacggcaact acaattggtt cgccaatgcc accagcgaca
     9841 catttggcag cggcagtttc gtcgtgccgg tggttgccga ttacagcacc aaggcgatac
     9901 ccgaaggcac ttcattgcgc atcgacgtcg agctgcaaca tcaaagcggc agcggtggcg
     9961 ggttttacac cgccgcttca acctcgacct atctgactgc ggctgataag ttatgcttca
    10021 acaaatgctc ggtgacgatc acctctcgtg ataaggcgcc cgccagcgga acgatcacct
    10081 tgcgcagtca cttcggatcg tggttcaggc ctgaaggctg gctgcacgct gccatcccgg
    10141 tcaatgccaa tcaattcgtg aaaaccacga tcgtcggcgt gccgtgcgat tggaccgtgc
    10201 gagcgtggta ttacccgtcg accggatcga atcgcacgcc gaagctgctg ccctcacaat
    10261 attggccggg cgacttcgct gcatcgacct cggatggcgc gctgccttac gcagctgcct
    10321 ttgccaatgg actcgtcgcg accaaaccct tggagtcgga cgatccgtac gcaccgaagt
    10381 aattaatcaa attgaaattc ctgcgccctc gatcggctgt acgcgcgatc gagggctttt
    10441 tgttttcaat taggtcatga ccagacgcta acgatctttg agtggttctt caatgagccg
    10501 ggcgcgggcg taggttggga atcggtacag cagacgattg cgttcagcga tgagattcaa
    10561 caagaggata ttgagatttg tgagacggtg cagcacggct tgcaatcgcg cacttaccat
    10621 cgaggccgct tctcagtcaa gggtgagaac ggcgtgcatc attttcaacc gctgctgtat
    10681 gagtttgtga cgaagtgaat aagacctcgc aggttttgaa aacctgcgag gactcactaa
    10741 acttggctgt tgactcgact gactgcgcct aaggtcaaac acacaggaca tgttgcgcta
    10801 tgccagactt caaacgattc agcgacaagt tggcgtcgtg ataacgccgg ccgctgtctg
    10861 cggcggatga taatccacat accgagactg agcaagacca acacgctgat cgctacgatc
    10921 aagattggca gcgggatcac ttgagcaaat tggctcacca acgagccgat gattgcgccc
    10981 aagaagaagt gtagactcaa gaacaagcct tcgccaatca acaagcccac tgtaaatttc
    11041 ccgatcggca cgtcggcgat tccgcaagcc ggcactgaaa tggcacgcac gcccggcgtt
    11101 aagatgatca accccaaacc gatcggattg cgctgcttca cgatcgacgc agccgcatcc
    11161 aagcgcgtgg ccgtcaagcc caaatatcgt ccgaagcggt acaaaaattt tcgacccggg
    11221 ccgcgcacga tcaaaaactg aatcaagccg ccgatcaaca acgcgatcag cacacttaca
    11281 aaggcttgcc acaaaatgaa tttgccttcg accacgcgcg ccgaagtcgc cagcatgatc
    11341 acatcggccg gaatcgggat cggcacaccg atcgccttga tcaacatcac cgcgaagatc
    11401 gcgatcaagc catacgtatt gaggaaagtt tcaatgctgc tcatgcgaaa gacttaggcc
    11461 tcgcgtcgca gaatgacgcc cagccagagc cagaagatcg gcccgccaac cgcacccgcg
    11521 atcaatgacg cgatcgttgc caccagatcc atgcccaaga tcgatccgat aaagcccacg
    11581 gctaaatcgg cgaaagcgat caaacccaaa tagcccaaca actttgggaa attcgagcgc
    11641 agcatcaaca acgcggcgat taagaaccat acactggtca agccaaaggt catcacgcca
    11701 aatggattga ccggcgaagg caacaggaat tctgcgcgcg cgaattcggg attcgtcgcg
    11761 aaggcgacgg ccacatagtg cagatgcccg atcagataca gcgagccgac aatcgatccg
    11821 atcgaagcag ccaacccgac aagcgtggcg aagaaggccc agccttcttt caccgatcgc
    11881 aatcgatagt acaacgcgac ggtcccgaac aaggcccaga aagcgttcag cgcatacaac
    11941 ccgtaa
//
LOCUS       DASRTJ010000674         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_368346,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000674 DASRTJ010000000
VERSION     DASRTJ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368346"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..421
                     /locus_tag="VFF70_13655"
     CDS             128..421
                     /locus_tag="VFF70_13655"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HZY45780.1"
                     /translation="MSLILFGNRKEYAQSPKRSGEAIRMQAKRFGYFPRTFVWRGREY
                     NVEAVERCWTTGSRRNGGQMQRHYFQVRCPEGKFDLYQVIAYNTWHIARIERQ"
     gene            490..1212
                     /locus_tag="VFF70_13660"
     CDS             490..1212
                     /locus_tag="VFF70_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY45781.1"
                     /translation="MKTYALYLESGPKKQRSMVHVLELLGCIAKGSTTDEAIANTPDA
                     IRAYLKFLRRHARSAPIDLGGALKTKIVEHVMAGQWLGNGDPALVFQFDLEPITPKEI
                     ETYLQQLEWSRAEVIKLVSGLTHAQLEFDPKNKQRPIRIMLEHMLESEYFYLSSFLGP
                     IEGLPATGSIVRKREGDLLQWMSYVRTIEVERLQTLTTEERSKSIEHWKQTWTARKMF
                     RRMLEHEWEHLIELSERLGKKI"
     gene            1271..1744
                     /locus_tag="VFF70_13665"
     CDS             1271..1744
                     /locus_tag="VFF70_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY45782.1"
                     /translation="MEPIEILQFMESVRAKTLKRFNGLNQPQLDWKPPKGDGEEAWSL
                     GEVFMHLALDEHYLREHIARPLLEGIKPPEGITFLPPPPPYGASSEVIAFWFDRARTM
                     TRRMIENWPANANLELKHFGGFDESANGAEWLMGYAGHEKFHHKQIDALIAQVSR"
     gene            1751..2239
                     /locus_tag="VFF70_13670"
     CDS             1751..2239
                     /locus_tag="VFF70_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45783.1"
                     /translation="MQIFHWQLEAPAVCHRDVVIGQANRIEMKYNSTKDLKKIETLRY
                     LYLKRLYELSDGSALQYVAFASVSRSLGWDDETTVKVTAYLKGESLVEFPASGKVYIT
                     HKGIKWIEKALSRFDRSTRHSKQAGFVTFMAEGDISIGGDVVGRDKKTKSSKRSRGRD
                     QK"
BASE COUNT          605 a          589 c          653 g          491 t
ORIGIN      
        1 ctgccaccac aatcaaatcc gggctacgca gctcaatcga tcaataacaa ctggggcggc
       61 gcctatccgc tggcacaaaa tacgtcgccc cgacaattta ccattgttgg agaggagcca
      121 accgatcatg agtctaattc tatttggtaa ccgcaaagaa tatgcccagt cgcccaagcg
      181 cagcggcgaa gcaatccgca tgcaggccaa gcgcttcggc tattttccac gcacgtttgt
      241 gtggcgcggc cgcgagtaca atgtggaagc cgtcgagcgc tgctggacca ccggcagccg
      301 gcgcaacggc ggacagatgc agcggcatta ttttcaagtg cgctgcccgg aaggcaagtt
      361 cgatctgtat caagtgatcg cctacaacac ctggcatatc gcacgcattg agcgccaata
      421 gcgcgcgtgc aagacaatcc gccgctatca cgcggcggat tttgttttga gtgtgttaaa
      481 cttgggtcga tgaaaactta tgcgctctat ttagaatccg gcccgaagaa acaacgcagc
      541 atggtgcacg tactcgagct gctcggctgt atcgcgaagg gatcgacgac cgatgaagcg
      601 atcgcaaata cgcccgatgc gatccgcgcc tatctgaaat ttctaaggcg ccacgcacgc
      661 tccgcgccga tcgatctcgg cggcgcattg aagacgaaga tcgtcgagca tgtgatggca
      721 ggccagtggc tgggcaacgg cgatccggct ttggtctttc aattcgatct tgaaccgatc
      781 acgccgaaag agatcgagac ttatcttcag caacttgaat ggtcgcgtgc cgaggtgatc
      841 aaattggtca gcggcttgac gcacgcgcag ctggagttcg atcccaaaaa caaacagcgg
      901 ccgattcgta tcatgctaga acacatgctg gaatcggaat atttttattt gagttcattt
      961 ctgggcccaa tcgaaggctt acccgccacc ggatcgatcg tgcgcaagcg cgaaggcgat
     1021 ctactgcagt ggatgagcta cgtccggacg atcgaggtcg aacggctgca aaccttgaca
     1081 accgaagaac gatcaaaatc gatcgagcat tggaagcaaa cgtggacggc gcgcaagatg
     1141 ttccgtcgga tgctggaaca tgagtgggag catttgatcg agttgtcgga gcgtttgggc
     1201 aaaaagattt agatagaaga tagaatacag aattcagaag ataagttcgc ctcgccaagg
     1261 gagcaaccgc atggaaccaa ttgagatttt gcaattcatg gaatcggtac gagccaagac
     1321 gcttaagcgt tttaacggtc tcaatcaacc gcaactcgat tggaaaccgc cgaaaggcga
     1381 tggcgaagaa gcgtggtcac tcggcgaagt gtttatgcac ttggcgctcg atgagcatta
     1441 tctgcgcgag catatcgcgc gaccgctgtt ggaaggcatc aagccgccgg agggaatcac
     1501 ttttctgccg ccgccaccac cctatggcgc atcgagtgaa gtgattgcct tctggttcga
     1561 tcgggcgcga acgatgacgc gccgcatgat cgagaattgg ccggcaaatg cgaatctgga
     1621 actgaaacat tttggcggct tcgatgaatc ggccaatggg gcagaatggc tgatgggcta
     1681 tgccgggcac gagaagttcc accacaaaca gatcgatgca ctaatcgcac aagtttcccg
     1741 ctgatgtcgt atgcaaattt ttcattggca acttgaagca ccagctgtgt gccaccggga
     1801 tgtcgttatt ggacaggcca atcgaattga aatgaagtac aattcaacga aagatctgaa
     1861 aaagatcgag acgctgcgct atctttacct caaacgtctc tacgagttaa gcgatggtag
     1921 tgcactgcaa tacgttgcgt ttgccagtgt cagccgcagc ttgggttggg atgacgagac
     1981 tactgtgaaa gtgaccgcgt atctgaaggg cgaaagtttg gtcgaatttc ctgcttctgg
     2041 caaggtgtac attacccaca agggtatcaa atggatcgag aaagcgctaa gccgcttcga
     2101 tcgatctacg cggcattcta agcaggccgg gtttgtcacg ttcatggccg aaggcgatat
     2161 ctcgatcggt ggcgatgtcg tcgggcggga caagaaaacg aaatcgtcaa agcgatctcg
     2221 cggccgcgat cagaagtaaa cttctcaact cggaggacga agatggttga ccccttgaaa
     2281 caacttctca aaaaatatcc gcccgatacc cgacggctgg tgcaggaaat gcgcaggg
//
LOCUS       DASRTJ010000675         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_294032,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000675 DASRTJ010000000
VERSION     DASRTJ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294032"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(669..>2617)
                     /gene="dhaR"
                     /locus_tag="VFF70_13675"
     CDS             complement(669..>2617)
                     /gene="dhaR"
                     /locus_tag="VFF70_13675"
                     /inference="COORDINATES: protein motif:HMM:NF008485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HZY45784.1"
                     /translation="DHGVIDPRIDPIVTMSWRRCRPMHNPRTAGNLPRLSAESLQSLL
                     INHFNLIAIARPFMEDIYQFVEGSGQIVVLLDASACVLEVLGDGDMRAEAATLGMQPG
                     TYWDEGHAGTNAFGLAMLEGSPGQVIGAEHLFERFHSLASAAAPIFNSDGHPILILGL
                     VSRARDSELHTLGAVYAAARAIGTQIQAEQLFIQVNEQRTQLEATLEAIAGGLVVIDA
                     GRLVTEMNAIAAEILNLKYEAVVGRPLSEYVDLPSVVQESLRSQHPLNEVEVIFQVKG
                     LQIGCLINLHPIRYEPGAKVDGWILTLRKIEQVHKLVQRMVGARATLTLTDFVGESSV
                     VNNIRRQAQIAARSQSPILVVGETGTGKGVVARAIHNESARADGPFITINCRVLPRDI
                     VVREFLGYEGSAFRKTGSFGQPSKFELTHGGTLHLEDIDALPLEMQTALLRVIDTGEV
                     MRLGGTRIIPVDVRFIATTNANLERAVANGDFRADLYYALSRMIIRLPALHDRMSDLN
                     LLVEAALDRLQRQTGHRIEISPEAMQVLRRYRWPGNNRELENALERAANLSEYGLIEV
                     KHLPENIRGRSSKAAQEEHVPALHEAEHEAIMRAGWACNGSLTKTAAMLGIGRTTLWR
                     KMKAAGIRVEDLRKPLEARSNRPH"
BASE COUNT          628 a          739 c          678 g          572 t
ORIGIN      
        1 ccattgccca agagaatcag aatcatcgtg ccgagaatct caccaactag catttcattt
       61 tcctccgatt ttgagatttt ggatttggtg ggtgatgcga gagacatagc caactgcacg
      121 ttgatgcgaa ctgagggaca ccttggtttc cgttgcgatc atcctccttt ccgccagcac
      181 gacgggcata cccaagcgta caaaaatgcg ttataaccgt gccgaaaata aaaagcgctg
      241 ttcaggggat aaacctaaaa cagcgcttta actgaactcg aactggacca attgcaaata
      301 gaaaaccgaa ttgtcgaagt gaggcgatcg agactaggac catactgccc ttcgactgcg
      361 atacgcttca tctgggctcc tcgaaagaac cgctaaccta taggctggta cgtcacgcgc
      421 gccgagtaat atggagttag gttatcatag gacatcagta cctgtcaatc gatccggcga
      481 aagtgtttca gagtggtacg cccagcccga ttgatttgtt tcgttttgga acaggccgtc
      541 gactagggaa taggtgatca tcaacccctt ttttatgtgt gtgatgacca aaaaagaaaa
      601 acaaccgccc ttgaagcgaa tacctcgagg acggttctag agcaaaatca aatcaccaaa
      661 attgcacttc aatgtggccg gttcgatctg gcttccaacg gctttcttaa atcctccacg
      721 cgaattccag ccgctttcat ctttcgccac aaagtagttc ggccaatacc cagcatcgcg
      781 gccgtcttag tcaggctacc gttacacgcc cacccagcgc gcatgatcgc ctcgtgctca
      841 gcttcgtgta atgcgggcac atgctcttct tgagcagctt tgctcgatcg gccgcggata
      901 ttttccggca gatgtttcac ttcgattagg ccatactcgc tgagattagc ggcgcgctcc
      961 agcgcattct ccaattcccg attgttgccg ggccagcgat aacggcgcag cacttgcatg
     1021 gcctcgggcg aaatctcgat gcgatgcccg gtttgccgct gaagccgatc cagggcagct
     1081 tcaaccaata gattgagatc gctcatccga tcgtgtaggg caggcaagcg gataatcatc
     1141 cggctgagcg cgtaatacaa atcggcacga aaatcgccat tggccaccgc gcgttccaga
     1201 ttggcgttgg tcgtcgcgat gaagcgaaca tccaccggga tgatccgcgt gccgcccaaa
     1261 cgcatgactt cgcctgtatc gatcacgcgc aggagagcgg tttgcatttc taagggcaag
     1321 gcatcgatgt cttcgaggtg aagtgtgccg ccgtgcgtca attcaaattt gctgggctgg
     1381 ccgaatgaac cggtcttgcg aaaggcactg ccctcataac ctaagaattc gcggacgacg
     1441 atatcgcgcg gcaggacacg gcaattgatc gtaatgaagg ggccatcagc ccgcgcgctt
     1501 tcgttgtgaa tcgcccgcgc gacgacgcct ttaccggtgc cagtttcgcc gacgactaaa
     1561 atcggcgatt ggctgcgtgc agcaatctgc gcctgacggc gaatattatt caccacgctc
     1621 gactcgccga caaaatcggt caaggtcaac gttgcgcgcg cgccgaccat gcgctgcacc
     1681 aatttatgca cctgctcgat cttgcgcagg gtcaagatcc agccgtcgac cttagcgcca
     1741 ggctcatagc gaataggatg caaattgatc aaacagccga tctgcagacc cttcacttgg
     1801 aaaataactt caacttcatt cagcgggtgt tggctgcgca aagattcttg caccacggac
     1861 ggcagatcga cgtattcact tagcgggcgg ccaaccaccg cttcatattt caaattcaat
     1921 atttcggcag cgatagcatt catttccgtg acaagcctgc cagcatcgat caccaccaga
     1981 ccgccggcaa tcgcttcaag tgtggcttcc agttgcgtgc gctgttcgtt cacctggatg
     2041 aataactgct cggcttgaat ttgcgtgccg atcgcgcgcg cggccgcata gaccgcgccc
     2101 agcgtatgca gctcgctatc acgggcgcgg ctgaccaaac ccaggatcaa gatcggatgt
     2161 ccatcagagt tgaaaatcgg cgcggcggca cttgctaaag aatggaatcg ctcaaagaga
     2221 tgctcggccc cgataacttg ccccggactg ccttccaaca tggcaaggcc gaaggcgtta
     2281 gttccggcgt ggccttcatc ccaataagtg cccggctgca taccgagcgt ggccgcttcc
     2341 gcccgcatgt cgccatcgcc taacacttcc aagacacagg ccgaagcatc gagcaatact
     2401 acaatttgcc ccgagccttc gacgaattga taaatgtcct ccataaaagg ccgggctatg
     2461 gcaatgagat tgaagtgatt gatcagcagg gattgaagcg attcagccga caaacgaggc
     2521 aggtttccgg cggtgcgcgg gttgtgcatc gggcggcagc ggcgccatga catggtcaca
     2581 atcggatcga tccgcggatc gatcacacca tgatcga
//
LOCUS       DASRTJ010000676        11855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_211547,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000676 DASRTJ010000000
VERSION     DASRTJ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211547"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VFF70_13680"
     CDS             complement(<1..1354)
                     /locus_tag="VFF70_13680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45785.1"
                     /translation="MSVTYLQSPSSLHRSITPSLILLSSLIFLLAFLPRLGAIDRYVT
                     PDELHWVDRSIRFTDAIAHGHWADTVQSGHPGATTMWLGSIGIAIQHLTNPAIPLPST
                     SITFDPQNAEAMRALAKFLDAARLPVILVTSFNIALLFLLLSRKIDRRAAFLAAGLIA
                     LDPFGVALGSILHVDSLLATFSLVSVAALFIALDHPKPMRWLIVSGVLAGLAALSKSP
                     AIVLGPTTFIVLAVSYWRQQRSIGSFFRSFFIWGICATLVFFALYPAMWVKPIAALGR
                     MAATAEKFGEFAHAVNYFFGSLDRDPGPLFYPVVIAFRSTPILWVGLIAVVPLIFRPR
                     TVGKFVTLSEAQGLALSAEMLRSAQHDKQKNNHEKCLRSIALSLLLFAFLFIGLITLG
                     AKKLDRYVFPALLALDIVGALGLAYAIRWIADRLKDRSRVATSIAIAACFIIASIQFM
                     D"
     gene            complement(1369..3036)
                     /locus_tag="VFF70_13685"
     CDS             complement(1369..3036)
                     /locus_tag="VFF70_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZY45786.1"
                     /translation="MTFNHKALIIGLDGATWTLLRPWIDQGHLPNLARLVREGASGPL
                     TTTIPPVSASAWVSFATGCNPGKHGAFDFVFPVAGGYDIGVINVKVRGVPPFWKSIEG
                     AGGQVGLMSVPITYPPEPINGFTVNGFLVPNEQSTYTYPPELKEELKHNGQRWPLHEF
                     EGNRSRHPGRFLQDMLDFDVTRTDTIIWLMQHKSWDTVACVLKTPDTVHHELWHIIDP
                     THPRYAAELNRQFAPAVLNYFQKIDECVGRIIAAAPPDTFVTIMSDHGGGPFHKFFHI
                     NNWLAQQGLLKFKRTPLSLIKRVLFKLGFTPITSLKIVNLLRLGRLRRRVKRGRGRGV
                     LKRLFLSFGDVDWSRTKAFAVGNFGQIYINVKGQRSQGIVEPGVEYEAVRDQIIRSAL
                     ALRDPNGGDQVIAAVYKKEELYHGDRVPVAPDLILHTDRSKYVSFGHADFGSNKLLEP
                     SIGQTGHHTMDGIVVLHGEGVIANTAIKGNIIDIAPTVLYSLGLPIPAIMDGQVIQSA
                     FSAEYLAAHPIVRAAADEPASSGSTKVYSVEDEAQVMDRLRELGYVA"
     gene            complement(3107..4066)
                     /locus_tag="VFF70_13690"
     CDS             complement(3107..4066)
                     /locus_tag="VFF70_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZY45787.1"
                     /translation="MKLGRILRVLISLIILVIVIAGVGADRLFDALRSINLGWFAIAL
                     LFHLIGLVVRTYRWSILIKALGTPVSFGRLLYLYLAGTFFNTFLPTGIGGDVVKIIEL
                     APDRGGAQAFSGVFADRLTGILGSSLIALIVAVLDPADVPAYVVTTVILISAGILIAS
                     FLLTQRRFFEWLIARSSFWPKIPGAKKVQKVVEAVTSYSIGTIVRALLVSLPFTLTLI
                     ATQYALALGLGVNAPLRYFALFVPMVALVQLLPISFNGLGVREGTYQLLFGTVGVEAP
                     QAVAMSLMYYIVRVVGGLFGGLLYLIGNLKADRATVKPPEKIA"
     gene            complement(4178..5836)
                     /locus_tag="VFF70_13695"
     CDS             complement(4178..5836)
                     /locus_tag="VFF70_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZY45788.1"
                     /translation="MKLIIIGLDGATFDLIKPWAAEGKLPTLARLMQDGVHGNLESTL
                     PPVTSPAWPTFMTGKNPGKHGVFDFIRPRAGSFDMVNASQIHGQLLWEILSEAGYSVG
                     VLNVPITYPPRKVNGYMVPGLLSPDQGKTVYPANFLKTYEAELGKYRLTPNVQYKPGN
                     EDEFIADIHDLIDTQLRYALRLMHDHPTDVLMLHFLASDNGSHALWRFMDKTHPRYDP
                     ALAEKYGDALQKLYQHLDQAVAQVIDNARSTAASGQQSAVDVIVMSDHGFGPLHRTIN
                     LNILFLEKGLMALKPKFFTRLRYWAFRHGLTPAGVYKVLSQLGLQNITARVSRKARNE
                     VVGKFLSFEDVDWSQTIAYSMGHVGQIYLNLKGREPFGIVGPDRYIAARQKVIDALNT
                     LIDPDTGKSMVDRIIPREEAAHGPYADQGADLHLILDDYKTIAFPLFATEGRVLTPQI
                     RGDSGCHRLYGIFIGNGAAFEKGVEVSSARIIDLAPTILHILNIPVPTDMDGRVLSAA
                     LTPELRSRTTQISAAAQSTHTQVDLSDAEQAEVEERLRALGYLG"
     gene            complement(6413..8947)
                     /locus_tag="VFF70_13700"
     CDS             complement(6413..8947)
                     /locus_tag="VFF70_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45789.1"
                     /translation="MNSSLKQTLRRDLLVLLFFTALTLFMTYPLITKIGSVYAGNNED
                     LWTFQWDNWWTRTALSHGLDTLYTNYQFYPNGVSLAAHSLSFYNSILWIPLSAIFGDI
                     AAYNITILLTFILSGYTMFKLADYLLTADSRRQTADGHDPTAVGGQRSSVAALIAGII
                     YAFAPYHFSQSLGHVSLASVQWFPLLALFILKATRERSWRNAIAIGLVTFLLTATRLQ
                     FIVLGAAVFAIFVAVDWIVSHREWVRGAIVRLIVGAAIGLILSLPIALPAAQLYAGSA
                     SPDELIADEQTWGQTDLLAYALPMTYHPIFGPIVQPLYTHFAKNQAWMPYLGLIVVAF
                     AVWGSARSKRRSLPWLVIGLFLFIMALGPFLLVNGVQYQNIRLPYALIGDKFPLNTLR
                     SPDRYNLLLPLVLATLAAFGIADILKRLSGRGKTIGAMLIGGMIVFEYLGLPYPTIEP
                     LPSSLFYATIAHDSTPYAILDVPLARSDTKRYLYYQTLHNHPIIEGRVARVPDEAYQL
                     FNSIPLLSQWRHTLDAKRPIDLGAQLNLLAQQNIHYIILHKDLATSLQTAQLRDYFTR
                     EPVFEDDQIAVYSTQPIEDSITAIGGSIGNINSWVNVSGPDQPIEVRMRWSSTQVIDR
                     DYSYRLALIDPAGSIVLSSTDRLTPTTSTWLTGTLVTPVYRLIPKTRVPIGQFKLQLN
                     ILDADQVLGSIDLPQRIVNDQNWIMSVTDEPHARFGSSIELRAADVSRRGNVLVLWLH
                     WHALTTPGVDTKYFVHILDANGDVVAQDDGVYGQYTKPSSEWQAGQYISDQLEIPLWN
                     LIPGDYRISIGVTDPETNERLNAIDSARQPLDQNRYIFSEMIKINK"
     gene            complement(9011..9994)
                     /locus_tag="VFF70_13705"
     CDS             complement(9011..9994)
                     /locus_tag="VFF70_13705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45790.1"
                     /translation="MDQPTLDISIVIPVYNEAEALIDLHSELDAALKSIGKSYEIIAI
                     DDGSHDDSLKVLKQLSAIDPQLVVISLRRNFGQTAAFAAGFDRARGSIVITIDADGQN
                     DPADIPALLKKIDEGYDIAAGWRQHRKESLITRKIPSRAANWLIAKQTGIYLHDSGCS
                     LKAYRWDVIKNVRLYGDMHRFIPAFASWLGVRVAEVPVKDRARKFGKSKYGFSRTFRV
                     FLDIFTLTFLLSFQGKPMRLFGSVGLITSSLGGIILAILAYLKIFRGELLSNRPILWL
                     GIMLVILGVQFLFFGLIAELQTRTYFESQNKSIYVVRETIEHGQSDGAKEG"
     gene            complement(10282..10737)
                     /locus_tag="VFF70_13710"
     CDS             complement(10282..10737)
                     /locus_tag="VFF70_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZY45791.1"
                     /translation="MPNLVHRPNLVQRLIQYLAALRAMSWFLAHVLHHLDRAIDRVSR
                     GRINLTQALTGLPVVMLTTIGAKTGQHRIVPPVAIPDGDTIILIASYFGNQHHPAWVY
                     NLRAQPEAIVLDHERSGTYIAREAIEAERDRYWQRGECVCGLRSLSTPG"
     gene            complement(10815..11465)
                     /locus_tag="VFF70_13715"
     CDS             complement(10815..11465)
                     /locus_tag="VFF70_13715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZY45792.1"
                     /translation="MLPIGDDDSARRTVPLVTYALIALNVLFFFVELSGGDAFIGKWA
                     FVPKRFLANPGADFLTLFTSMFMHAGWVHLGGNMLYLWIFGDNVEDRFGHIKFILFYL
                     LCGLAATFAQLAFSLGSNVPNLGASGAIAGVLGAYILLFPQGRVRVMQGQQVVQVPAL
                     IVIGLWIVLQLFSGIGSIASTADTGGVAYMAHIGGFIAGFVLTFVFRGNRGPQATG"
     gene            complement(11619..>11855)
                     /locus_tag="VFF70_13720"
     CDS             complement(11619..>11855)
                     /locus_tag="VFF70_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45793.1"
                     /translation="GQSSNLIISTADGKVVVKSGTQRPQINLLVRIVYFVLIGWWLSA
                     IWMELAYAVCATIVGMPIGFWMFDRVPAILSLKQ"
BASE COUNT         2860 a         3141 c         3312 g         2542 t
ORIGIN      
        1 cgtccatgaa ttggatcgag gcgatgatga agcaagctgc aattgcgatc gatgttgcaa
       61 ctctcgatcg atccttcaac cgatcggcga tccacctgat cgcgtaagcc agccccagcg
      121 cgccgacgat gtccagtgcc aataaggccg gaaacacata gcgatcgagt ttcttcgcgc
      181 cgagggtaat caaaccaatg aacaaaaacg caaagagcaa aagcgataat gcgattgagc
      241 gtaaacactt ttcatgattg ttcttttgct tgtcatgctg cgcggagcga agcatctcgg
      301 ccgatagtgc gagaccctgc gcttcgctca gggtgacaaa cttgccaact gttcgcggcc
      361 gaaagatcaa cggaactacc gcgatcaatc cgacccatag aatcggcgtc gatcgaaacg
      421 cgatgacgac aggatagaac agcgggccgg gatcacggtc gaggctgccg aagaaataat
      481 tgacggcgtg tgcgaactca ccgaattttt cggcggttgc ggccatgcgc cccagcgccg
      541 cgatcggctt gacccacatc gccggataaa gcgcaaagaa aacaagtgtg gcgcagatgc
      601 cccagatgaa aaacgatcga aagaaagaac cgatcgatcg ctgctgacgc caataactga
      661 ctgccaggac gatgaaggtc gttggcccca aaacgatcgc gggtgatttg ctcaatgcgg
      721 ccaagccagc taatacaccg gacacgatca accagcgcat cggcttggga tgatcgagcg
      781 cgatgaacaa ggccgcaacc gaaacgaggc tgaaggttgc caacagcgaa tcgacatgca
      841 aaatgctgcc taacgccacg ccaaacgggt cgagtgcgat caagccggcg gctagaaatg
      901 cggcgcgtcg atcgatcttg cggctgagca gcagaaacaa caacgcgatg ttgaatgatg
      961 taacgaggat aacgggcaag cgcgcagcat cgagaaattt tgccagtgcg cgcattgcct
     1021 cggcgttttg tggatcgaag gtgattgaag tgcttggaag tggaatggca ggattggtca
     1081 gatgttgaat ggcaatgccg atcgagccga gccacattgt cgtcgcgccg gggtggccgc
     1141 tttggaccgt atccgcccaa tggccgtgcg cgattgcgtc ggtgaaacgg atcgatcgat
     1201 cgacccaatg cagctcgtca ggcgtgacgt agcgatcgat cgcgccgagg cgcggcagaa
     1261 atgcgagcag aaagatcaga ctgctgagca gaatgagcga tggagtaata gagcggtgga
     1321 gcgaagaagg agattgcaaa taggtgacac tcaaattcaa aaatcaggtt aagccacgta
     1381 gccaagttcg cgcaatcggt ccatgacttg cgcttcatcc tctacggaat acaccttggt
     1441 cgatccactt gaagcaggct catcggcagc cgcgcggacg atcggatgtg cagcgagata
     1501 ttctgcgctg aaagctgatt gaatgacttg tccatccatg atcgcgggaa tcggtagtcc
     1561 caatgaatac aacaccgtcg gcgcaatgtc gatgatgttg cccttgatgg cggtattcgc
     1621 tatcacgccc tcgccgtgca ggacaacgat gccatccatc gtatgatgtc cggtctggcc
     1681 gatgctcggt tccaagagtt tgttcgatcc gaaatcggcg tggccgaagg aaacgtactt
     1741 cgatcgatcg gtgtgcagga tcagatcggg cgcgaccgga actcgatcgc cgtgatacaa
     1801 ctcttccttc ttatacaccg ccgcgatcac ttgatcgccg ccgtttggat cgcgcaacgc
     1861 taacgccgat cggatgatct gatcccgaac ggcctcgtac tccacgcccg gctcgacgat
     1921 gccttgcgag cgttggcctt tcacgttgat gtagatctgc ccgaagttgc cgaccgcaaa
     1981 ggctttggtg cgtgaccaat cgacatcgcc gaatgacagg aacaagcgct tcaacacgcc
     2041 gcgtccgcgg ccgcgcttca cacggcggcg caggcgacct aagcgcagca agttcacgat
     2101 cttcaatgaa gtgatcggcg tgaagcccag cttgaacaac acgcgcttga tcaaacttaa
     2161 cggcgtgcgc ttgaacttta gcagtccttg ttgagcaagc cagttattga tgtgaaaaaa
     2221 tttatggaac ggtccgccgc catgatcgga catgatcgtc acaaacgtat cgggcggcgc
     2281 ggcggcgatg atgcggccga cgcactcgtc gatcttctgg aagtaattca gcaccgcggg
     2341 cgcaaactgt cgattgagtt cggcagcata gcgcggatgc gtcggatcga tgatgtgcca
     2401 gagttcatga tgcacggtat caggtgtctt caatacacag gccactgtat cccacgattt
     2461 gtgctgcatt agccagatga tcgtatcggt gcgcgtcaca tcgaagtcga gcatgtcctg
     2521 caaaaagcgg cccggatggc gtgatcgatt gccttcgaat tcatgcagcg gccaacgctg
     2581 accattgtgc ttcaactctt cttttagttc gggtggatac gtgtaagtcg attgttcatt
     2641 cggcacaaga aaaccgttga cggtgaatcc attgatcggt tcgggcggat acgtgatcgg
     2701 cacactcatc aaaccaactt gtccgcccgc cccttcgatc gatttccaga atggtggcac
     2761 gccgcgcacc ttcacattga tcacgccgat atcatagccg cctgccaccg gaaacacaaa
     2821 atcgaacgca ccgtgcttgc ccggattgca gcccgtggca aacgacaccc acgcggaggc
     2881 ggacaccggc gggatggtgg tcgtcaatgg gccagacgcg ccttcgcgca ccaaacgcgc
     2941 caggttaggc aaatgtcctt gatcgatcca cgggcgcaat aatgtccacg ttgcgccgtc
     3001 gagaccgatg atcaatgctt tgtgattgaa ggtcataatt cttcgctttc gatttcagat
     3061 cgccgctgaa tgcagagatt ggcgattata gcatgagagc gatccgctac gcgattttct
     3121 ctggcggttt aaccgttgct cgatcggcct tcaaattccc gatcagatat agcagcccac
     3181 cgaataatcc gccgacgacc cgcacaatgt aatacatcaa cgacatcgct accgcttgag
     3241 gggcttcaac tccaaccgtc ccgaataaaa gttgataagt tccttcgcgc acacccaggc
     3301 cattgaatga gatcggcagc agctgcacca acgcgaccat cggcacgaac aacgcgaagt
     3361 agcgcagcgg cgcattcacg ccgaggccga gggctaaggc atactgtgtc gcgatcaatg
     3421 tcaatgtaaa cggcagcgat accaacagcg ctcggacgat cgtgccgatc gaatacgagg
     3481 tgactgcctc gacaactttc tgaacctttt ttgcccccgg aattttcggc cagaatgaac
     3541 tgcgcgcgat gagccattcg aagaaacggc gttgggttag caagaatgaa gcgatcaaaa
     3601 ttccggctga aatcaaaatc acagtcgtca cgacatacgc aggtacgtct gctggatcga
     3661 gcaccgccac gatgagcgcg atcaacgacg agcccagaat gcctgtcaac cgatcggcga
     3721 acacgccgct gaacgcctgc gccccgcctc gatcgggtgc gagttcgatg atcttcacca
     3781 catcgccgcc gatgccggtc ggtaaaaacg tattgaagaa cgtgcccgct aagtataagt
     3841 acaacaatcg gccgaatgag acgggcgtgc ccagggcttt gatcaagatc gaccagcgat
     3901 aggtgcgcac gaccaggccg atcaggtgaa acaacagcgc aatcgcgaac cagccgagat
     3961 tgatcgaacg cagcgcgtcg aacaatcgat ctgcgccgac gcccgcaatg acgatgacga
     4021 gaatgatcag gctgatcagg acgcggagaa ttcggccaag tttcatatgc aatcaaaaac
     4081 cacagagaca cgcaggcaca gaggcagtct gaaaactctg tatctccggt acgcactgcc
     4141 tgctttaccg cacgtgtgtg actctgtggt ttgcctttta accaagataa cctaacgcgc
     4201 ggagccgctc ttcaacctcg gcttgctcgg cgtcactcag atcaacttga gtgtgtgtgc
     4261 tctgagccgc tgcgctgatc tgcgttgtgc gcgatcgaag ttcaggcgtg agcgcggcgc
     4321 tcaatacacg accatccata tcagtgggaa cagggatgtt cagaatgtgc aggatggtcg
     4381 gcgccagatc gatgatgcgg gcgcttgaaa cctcaacgcc tttttcaaag gccgcgccgt
     4441 tgccgatgaa gatgccatac aatcgatggc agccgctgtc gccacgaatc tgcggcgtga
     4501 gcacgcggcc ttccgtcgca aacaacggga aggcgatggt tttgtaatcg tccaggatca
     4561 aatgcaagtc tgcgccttga tcggcatacg ggccgtgcgc ggcttcttca cgcggaatga
     4621 tgcgatcgac catcgatttg cccgtgtccg gatcgatcaa ggtgttcagc gcgtcgatca
     4681 ctttttggcg ggctgcgata taccgatcgg gtccgacgat cccgaaaggt tcacggccct
     4741 tcaagttgag gtagatctgt ccgacgtggc ccatcgaata cgcgattgtt tggctccaat
     4801 cgacatcttc gaacgacagg aacttgccga cgacttcgtt gcgcgccttt cggctgacgc
     4861 gcgcggtgat gttctgcaag ccgagttggc tcaacacttt atatacgccc gcgggggtga
     4921 gaccatgtcg gaaagcccag taacgcagcc gcgtgaagaa ttttggtttg agagccatca
     4981 agcctttttc aagaaataaa atattgagat tgatcgtgcg atgcagcggg ccgaagccgt
     5041 gatcggacat gacgatgaca tcgaccgctg actgctgccc gctggccgca gttgatcgcg
     5101 cgttatcaat gacttgcgcc actgcttgat ccaaatgctg atacagtttc tgcagcgcgt
     5161 cgccatattt ttcggcgagc gccggatcgt agcgcggatg agttttgtcc atgaagcgcc
     5221 acaacgcgtg cgagccgtta tcgctggcga ggaagtgcag catcaataca tccgtcggat
     5281 gatcatgcat caagcgcagc gcgtagcgca gctgcgtatc gatgagatcg tggatgtcgg
     5341 cgatgaactc gtcttcgttg ccgggcttgt actgcacgtt cggcgtcaag cgatatttgc
     5401 cgagttcagc ttcgtaggtc ttgaggaaat tggccgggta taccgtctta ccttgatcgg
     5461 gcgagagcaa gccggggacc atgtagccgt tcactttgcg cggcggatat gtgatcggca
     5521 cattcaacac gccgaccgag tagcctgcct cgctcagaat ttcccacagc agctggccgt
     5581 gaatttgcga ggcattgacc atgtcgaacg atccggcgcg cgggcgaatg aaatcgaaga
     5641 cgccatgctt gcccggattc ttgccggtca tgaaagtggg ccacgctggt gaagtgaccg
     5701 gcggtaaggt ggattcaaga ttgccgtgca cgccgtcttg catcaagcgc gccagtgtcg
     5761 gcagtttgcc ttccgccgcc cacggtttga tcagatcaaa ggtggcccca tcgaggccga
     5821 tgatgatgag tttcatagtg agttaatctg gaagttggtt agttgagtaa tgagatagtt
     5881 gcttggttga atagtgatta gtaatcaaac tagacgactg atgttaatgg ttaagctgca
     5941 cacttttcac ttcaatccga tattcagcat cttgtgctaa ctgcatttta acatttgctt
     6001 ttgttgggta gccgtaattt gaatcgtatt caaacgagca tatcagaata ggaaacaata
     6061 aagagcattt taggccgtcg ttgaacatgc tttcaatggt taatctttga taaacgttta
     6121 acttacatgg atatatgccg caatagccgt cgttctcgcc atataccact tggcccttgg
     6181 acactgtgac gatattcttg ccttcatttg gatctaggct attcaagaaa gcggttatag
     6241 tgtaattctg cgaatcatac gataaccact tcgcgcgatt gaattcgcga atccctgaag
     6301 tgataataaa gttgattata tacggcgcta gaattattaa agatgcgatc aaacatcctc
     6361 ggcggcgacc ttttggcttc cggctattga tagaggtgct catctcattc atttatttat
     6421 tgatcttgat catctcggaa aagatatagc gattttgatc cagtggttgc ctggctgaat
     6481 ctatcgcgtt caatctctcg ttcgtttctg gatctgttac gcctattgag attcgataat
     6541 cgccggggat caaattccac aacggaattt cgagttgatc gctgatgtat tgtccggcct
     6601 gccattcgga actgggcttg gtgtattggc cgtacacgcc atcatcttgc gcgacgacgt
     6661 cgccattcgc atccaagata tgcacaaaat attttgtatc gacgccgggc gtggtcagcg
     6721 catgccagtg cagccacaag actaacacat tgccgcgccg gctgacatcc gccgcgcgca
     6781 attcgatcga tgagccgaaa cgggcgtgcg gttcgtcggt gacggacatg atccaattct
     6841 ggtcgttgac gatgcgctgc ggcagatcga tcgagccaag cacttgatcc gcatccagaa
     6901 tattcagctg caatttgaac tggccgatgg gcacgcgggt ctttgggatc aagcggtaaa
     6961 ccggtgtgac gagtgtgccc gtcaaccacg tcgaggtcgt cggcgtaagc cgatcggttg
     7021 agctcagcac gatcgagcct gcgggatcga tcaacgccag gcgataactg taatcgcgat
     7081 cgattacttg tgtcgaactc caacgcatgc gcacttcgat tggttgatcc gggccgctga
     7141 cgttcaccca gctgttgatg ttgccaatgc tgccgccgat cgctgtaatg gaatcttcga
     7201 tcggctgcgt agagtatacc gcgatctgat cgtcttcaaa aacaggttcg cgcgtgaagt
     7261 agtcgcgcag ctgtgcggtt tgcagtgagg tcgctaaatc tttgtgcagg ataatgtagt
     7321 gtatgttctg ctgcgccaat aagtttagct gcgcgcctag atcgatcggg cgtttggcgt
     7381 ccaaagtgtg gcgccattgc gacaggagcg ggatcgaatt gaaaagttga taggcctcgt
     7441 caggcacgcg cgccacacgg ccttcaatga tcggatgatt atgcagcgtt tggtaataca
     7501 ggtaacgctt ggtgtccgat cgggcgagtg gcacatcgag gatcgcatac ggtgtggaat
     7561 catgcgcgat cgtcgcatag aagagtgatg agggcagcgg ctcgatcgtg ggatacggca
     7621 ggccgagata ttcaaagacg atcatgccgc cgatcaacat tgccccgatc gttttaccgc
     7681 gtccggacaa tcgcttcaaa atatcggcga tgccgaaagc ggcaagggtc gctaacacga
     7741 gcggcagcag caagttgtat cgatccggcg agcgcagtgt gttgagcgga aacttgtcgc
     7801 cgatcaacgc gtaaggcaaa cgaatgtttt gatattgcac accattgacc agcaggaatg
     7861 gaccgagcgc catgatgaag agaaacagcc cgatgacaag ccacggcagc gatcggcgtt
     7921 tcgatcgagc gctgccccac acggcaaatg cgacaacgat caaaccgaga tacggcatcc
     7981 aggcttgatt cttggcgaag tgggtgtaga gcggctgaac gatcggcccg aagatcggat
     8041 gataggtcat cggcagcgcg taagcgagca gatcagtttg gccccacgtc tgttcgtcag
     8101 cgatcaattc atcaggtgag gcggaacccg catacagttg agccgccggt aacgcgatcg
     8161 gaaggctgag gatcaagcca atcgccgcgc cgacgatcaa gcgcacgatc gcgccgcgga
     8221 cccattcgcg atgcgacacg atccaatcga ccgcgacgaa gatcgcgaag accgccgcac
     8281 ccagcacaat aaactgcaaa cgcgtcgcgg tgagcagaaa agtaacgagg ccgatggcaa
     8341 tagcattgcg ccacgatcgt tcacgcgtcg ctttgagaat gaagagcgcg agcagtggaa
     8401 accattgcac gcttgccagt gagacatggc cgagcgattg cgaaaaatgg taaggcgcga
     8461 aggcgtagat gatcccggca attaaagctg cgaccgatga ccgctgacca ccgaccgcag
     8521 ttggatcatg accatcggcg gtttggcgtc tgctgtctgc agtcaataag taatcggcta
     8581 acttaaacat cgtatagccg ctgaggatga acgtcagcag aatggtgatg ttgtatgcgg
     8641 cgatgtcgcc gaagatggcg ctgagcggaa tccagaggat cgagttgtag aagcttagcg
     8701 aatgcgcggc gaggctgacg ccgttcggat aaaactggta gttagtgtag agcgtatcga
     8761 ggccgtgcga caacgcggtg cgcgtccacc agttgtccca ttgaaatgtc cacaagtctt
     8821 cattgttgcc cgcatacacc gatccgattt tggtgatcag cggataggtc atgaacaacg
     8881 tgagggcggt gaagaacagc aggacgagga gatcgcgccg cagggtttgc ttgagagaag
     8941 aattcatgat gctgaagcga tggagcgggg gagagacgga gagaaagagc gaaggagtct
     9001 ctcgttcgct ctatccctct tttgctccat cggattggcc atgctcgatg gtttcccgca
     9061 cgacgtaaat ggatttattc tgcgattcga agtaagtgcg cgtttgcaac tcggcgatca
     9121 gtccgaagaa taaaaattgc acgccgagga tgaccagcat gatgcccagc cacaagatcg
     9181 gccgattgga cagcagctcg ccgcgaaata tttttaagta agccaggatc gccagaatga
     9241 ttccaccgag gctgctcgtg atcaaaccga cgctgccaaa caagcgcatc ggtttgcctt
     9301 gaaaactcaa caggaaagtt aaagtaaaaa tatccaggaa cacgcggaac gtacgcgaga
     9361 agccgtattt gcttttgcca aacttccgtg cgcgatcttt gaccggcact tcggccacgc
     9421 gcacgcccag ccacgaggcg aaggccggaa tgaagcggtg catgtcgccg tacaagcgca
     9481 cgtttttgat cacgtcccaa cgatacgcct tcaacgaaca gcccgaatcg tgcaaataaa
     9541 ttccggtctg tttggcgatg agccaattcg ccgcgcgcga tggaatcttg cgcgtgatga
     9601 gcgactcttt acgatgctgc cgccagcctg cggcgatgtc gtagccttca tcgattttct
     9661 tcaagagcgc tggaatatcc gctggatcat tctgcccgtc cgcgtcgatg gtgatcacga
     9721 tcgatccccg cgcccgatcg aagcccgcgg cgaaggcggc cgtctgcccg aagttacgcc
     9781 gcaagctgat gacgaccagc tgcggatcga tcgcgctcaa ctgcttcagc actttcaggc
     9841 tatcgtcgtg gctgccatcg tcgatggcga tgatctcgta tgacttgccg atcgatttca
     9901 gcgcggcatc gagttcggag tgcagatcga tcagcgcttc ggcttcgtta tacaccggaa
     9961 tgacaatgga aatatcgagg gtgggttgat ccatagttat cgtcaacaat cgacccgact
    10021 agtggtagtc gggtcgatgg ccgattataa catgattgag tgggcggtac ttatcaaagc
    10081 agggcttttt gtttgtcatt atgagcgaag cgaagaatct tgacgcaaat cgatcgagac
    10141 ccttcgcgga gtttacactg agcgcagtcg aagtgctcag ggtgacaatc ttgcgaccat
    10201 gcttagcgca atttgtttac gggcttcgtg ctgtctgtgg ttcaatctat cggtgtcagc
    10261 agcataatcg ggatgacgcg cttaacccgg cgtcgatagg ctgcgtagcc cgcatacaca
    10321 ctcaccgcgc tgccaatatc gatcgcgctc ggcctcgatc gcttcacgcg cgatataagt
    10381 gccgctgcgt tcatgatcga gcacgatcgc ttcgggttgc gcgcgcaggt tgtaaaccca
    10441 ggccggatga tgttgattcc cgaagtatga tgcgatcaag atgatagtat cgccatcggg
    10501 aatggcaacg ggcggcacga tgcgatgttg acctgtcttc gcgccgatcg tcgtcagcat
    10561 cacgacgggc aaacctgtca gcgcttgtgt caaattgatg cggccccgcg acactcgatc
    10621 gatcgcgcga tcgaggtgat gcaggacgtg cgctaagaac cacgacatgg cgcgcagcgc
    10681 ggcgaggtat tgaatgaggc gctgaacaag gttcggtcga tgcacgaggt tgggcacgat
    10741 tgaaagactt ctcttcgatg gcttcgcagt ttgctctccg actgcgtctc cgcaggcgtt
    10801 gagcaaataa cgcttcagcc cgtcgcctgc ggcccgcgat ttccacgaaa aacaaaggtc
    10861 aacacaaacc cggcgataaa tccgccgatg tgtgccatgt aagcgacacc gcccgtgtcc
    10921 gcggtactgg caatggaacc gatgccgcta aacaactgca gaacgatcca cagcccaatg
    10981 acgatcagcg cgggcacttg aactacttgt tggccctgca tgactctgac tctgccctgt
    11041 gggaatagca aaatgtaagc gccgagcacg cccgcaattg cccccgatgc tcccaaattc
    11101 ggcacgttcg atccgaggct gaacgctaac tgcgcaaatg tggccgccag cccgcacagc
    11161 aagtagaaga ggatgaactt gatgtgcccg aaacggtctt ccacattgtc accgaagatc
    11221 cataagtaca gcatgttgcc gcccaagtgc acccagccgg catgcatgaa catggaggtg
    11281 aagagcgtca gaaaatcagc gccaggattc gccaggaagc gtttgggcac gaaagcccac
    11341 ttcccgataa aagcgtcacc gccgctaagt tcgacgaaga agaacaggac gttcagcgcg
    11401 atcaaggcgt acgtgaccag cggaaccgtt cgacgcgcgc tatcatcatc accgatgggc
    11461 agcataagat ttttctcctt ttactccgat tggatttgtc acagattcac ttcaaaatga
    11521 gaccggcatt tttcaaagaa agtgatccaa tcacgatcaa catatctgca tgcgttgacg
    11581 gctccttctg ttctaatgat tatgctgatt tcgccctact attgtttcaa tgacaatata
    11641 gcgggcacac gatcaaacat ccagaagccg attggcattc caacgatagt cgcacacacc
    11701 gcataagcca gttccatcca gatcgcactg agccaccagc cgatgaggac gaagtaaaca
    11761 atgcgcacca acaagttgat ctgtgggcgc tgcgttccgc tcttgacgac gaccttgccg
    11821 tccgccgtgc tgatgatgag attgctgctc tgccc
//
LOCUS       DASRTJ010000677         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_211674,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000677 DASRTJ010000000
VERSION     DASRTJ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211674"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VFF70_13725"
     CDS             <1..1649
                     /locus_tag="VFF70_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZY45794.1"
                     /translation="QLPRGRFALLIMLLIGLTISVVMQVVRYQVFQHALPPDLAGGLD
                     QSIRVTQRGRIWDRTGSLLANDEPRYAVMFDPIRDQAVNEKLINQAARDLTPVLNMSP
                     ADFRSKLGLKDQHLGLIDNLTADVGKQVNSLQIPSFSAASYWGRAYPEGTLAASVLGF
                     VNAARIGFYGVEGRYNNLLSPQGSRPGADLVLTIDRTAQSITEEELANGLRDTGAEAG
                     SIIVMNPRTGEILAIATAPGYDPNHYSAIPQSEQSIFVDPAVSVHYEPGSVFKILTFA
                     AGIDSGTITPQTTYYDTACTEVGGQTMCNWDRAGHGLVTMVDMMARSLNVGAAYVSTH
                     MGQQNFYRYLKGFGVGKATGVDLQNEDSGELRTRDGNPDNWSEGDLGTNAFGQGLSTT
                     PLQLIAAVAAVANDGVMVQPHVVKQIRDGDQIRTAQIVELGRPISAQTAHTVSDVLVQ
                     VVQREVSNAQVSGYRIAGKTGTAQIALPGGYDPIYTIASFIGYAPADNPQVIILVKLD
                     RPTSSPWGSETAAPVFQKLATRLFPILGILPSTEQLAINN"
     gene            1659..>1906
                     /locus_tag="VFF70_13730"
     CDS             1659..>1906
                     /locus_tag="VFF70_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HZY45795.1"
                     /translation="MTLTLADVVEGLTGQRSQDATQNIRRFVIDSRETGPGDVFVALI
                     GDQTDGHLYVADAFKRGAIAAIVEQDLAAIEASTIDLRS"
BASE COUNT          420 a          484 c          576 g          426 t
ORIGIN      
        1 tgcaactacc tcgcggacga tttgctttac ttatcatgct cttgattggc ttgacgatct
       61 cggtggtcat gcaggtcgtt cgctatcaag tgtttcaaca tgccctgccg cctgatttag
      121 ctggtgggct agaccaatcg atcagggtaa cgcaacgcgg ccgcatttgg gatcgaactg
      181 gtagtctgct agcgaacgat gagccgcgct atgccgtgat gttcgatccg atccgcgatc
      241 aagcagtgaa tgagaagtta atcaatcaag cggcacgtga tctgacaccg gtgctgaaca
      301 tgtcgccagc cgactttcga tcgaagttgg gcttgaaaga tcagcatcta ggcttgatcg
      361 ataacttgac ggcggatgtg ggcaagcagg tcaacagctt gcagattcct agctttagtg
      421 cggcatctta ctggggacgc gcctatcccg aaggcacgct ggctgcttct gtactgggat
      481 tcgtgaacgc agcgcgcatt gggttctatg gtgtcgaagg ccgttacaac aacttgttaa
      541 gtccacaggg atcacgcccc ggcgctgatc tagtcttgac aatcgatcgg acggcgcagt
      601 cgatcaccga agaggaattg gcaaacggcc tgcgggacac gggcgcggaa gcaggttcga
      661 tcattgtgat gaatccgcgc actggcgaaa ttttagcgat agcgaccgcg ccgggttacg
      721 atccgaatca ttattcggct ataccacagt ccgaacaaag catctttgtg gacccggcgg
      781 tgagtgtgca ttatgaaccc ggctcggttt tcaagattct gacttttgca gcgggtattg
      841 attcgggcac gataacgccg cagaccactt actatgacac ggcttgcaca gaagttggcg
      901 ggcagactat gtgcaactgg gatcgggctg gacatggttt ggtgacgatg gtggatatga
      961 tggctcggtc tttgaatgtg ggcgcggcct atgtcagcac acacatgggc caacaaaact
     1021 tttaccgcta tttgaaaggc tttggtgtcg gtaaagccac gggtgtcgat ctgcaaaacg
     1081 aggattcggg tgagctgcgc acgcgcgacg gcaaccccga taattggtcg gaaggcgatc
     1141 tcggcaccaa cgccttcggt caaggactgt cgaccacacc gcttcagctg atcgcggctg
     1201 tggcggcagt ggcgaatgat ggtgtcatgg tacagccgca cgtcgtgaag caaatccgtg
     1261 acggcgatca gattcgcact gcgcagatcg tagagttggg tcgcccgatc tcggcgcaga
     1321 cggcgcacac cgtttcggac gtgttggtgc aagtggtaca gcgcgaagta tcgaacgcgc
     1381 aggtgagcgg ttatcgcatc gcgggcaaaa ccggcacggc gcagatcgcg ctgccgggcg
     1441 gctatgatcc gatctatacg atcgcttcgt ttatcggtta tgcgcccgcc gataatccgc
     1501 aggtgatcat tttggtgaag ttggatcgac cgacctcatc gccgtggggg tcagagacgg
     1561 ccgcgccggt atttcaaaaa ttggcgacgc gcttgttccc gattttgggt atcttgccca
     1621 gcactgaaca actagcgatt aacaattaac aaagatcaat gacgctgaca ctggctgatg
     1681 tggttgaagg actcacagga caacgatcgc aagacgcgac gcaaaatatt cgccgcttcg
     1741 tgatcgattc gcgcgagacc gggccgggcg atgtgttcgt ggcgctgatt ggcgatcaga
     1801 ccgacgggca cttgtatgtc gccgatgcgt ttaagcgtgg cgcgatcgca gcgatcgtcg
     1861 agcaagacct tgcggcgatc gaagctagca cgatcgatct cagatc
//
LOCUS       DASRTJ010000678         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_211729,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000678 DASRTJ010000000
VERSION     DASRTJ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211729"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFF70_13735"
     CDS             <1..367
                     /locus_tag="VFF70_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZY45796.1"
                     /translation="ATLEYLQKNGRIGGAAKLIGSALQIKPILQIDAGHVEAFEKVRT
                     HHKALERIREIARLDCPRTLESHLCVMHADAPSEANQLRVDLCADLGVCDIPLYSVGP
                     AITTHAGPGTLAIGFFVAN"
     gene            394..1308
                     /locus_tag="VFF70_13740"
     CDS             394..1308
                     /locus_tag="VFF70_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45797.1"
                     /translation="MSELPEIKKIERAVRRMTTVQFSELCSELGMIESLLGEDLNERT
                     KTLIGLQTAPDNLKHFVRTIRYIWPEAFDEPVTKPKRVIKIEPGQIIGGVALLIILFV
                     AALMIASAINPPSAPIAEFKVTPSIIATHVVLVAVRTPTPTPVTPTRTPAPTETPDVN
                     ATLTATFAPSPTETGTPTRTPKPTSQFTATPTPSATPAVQAIYPRVQLRKPVNNAIVS
                     INIKPLAQLQWIAPGELKPDERYWVRFRRNGSVAYAYFTSNNWFDGVPNNQLGAYQWS
                     VAIVKVDENGNVLGILGPESEAWNITWQ"
     gene            complement(1368..1958)
                     /locus_tag="VFF70_13745"
     CDS             complement(1368..1958)
                     /locus_tag="VFF70_13745"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HZY45798.1"
                     /translation="MEATSSTQPTSIPHKISKGIRIIAGRLRDQGLRTTGLWFTERTT
                     RILVGVSPERTSRVMKNLYVGGQHQRRGLKSMRARDITACIDLRREYGDVEHSLDLEH
                     HLSIPTDDDNVPSFEELIHAAEFIRDELAAGHGVYIHCANGVGRAPSIAAAYLITTGL
                     SAQAAWTTIRKARPFIRPTSVQREQIDRFEKQFRKD"
     gene            complement(2023..3150)
                     /locus_tag="VFF70_13750"
     CDS             complement(2023..3150)
                     /locus_tag="VFF70_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45799.1"
                     /translation="MDIIKIDTNDRRDVKRFLDFPFTLYKNIPQWVPPLDSDARRMLD
                     RRRHPFYQHSDADFFLALKNDRVAGRIAVLENRHYNEFNHTHQALFYLFEAEDDVEIS
                     RGLFTAAIDWARDRKLDEVFGPKGFTALDGMGLLAKGFEHRPALGIPYNPDYYPRLLE
                     DSGFEGLHDVVSGHLDPSHLNFPEKIHHISELVQKRRGLHIARYNSRRDLRTLIGPLK
                     QMYNDSLGGTEGNVPLTDDEVQTMADQLLSYADPHLIKIVMKDDRPIGFLFAYPDVSA
                     AIQRTKGKLFPFGWIDLLLEFKRTKWVNINGAGIVEQYRGNGGTAILFSEMYKSVKDG
                     GFQHADLVQIGADNDKMQREIASLGIDFYKMHRMYRKKILM"
     gene            3411..>3724
                     /locus_tag="VFF70_13755"
     CDS             3411..>3724
                     /locus_tag="VFF70_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZY45800.1"
                     /translation="MDSAIERILLDERSIAAKVKEMAGQISAAYQGVDELIVVCILNG
                     AFRFTADLEAHLTVPCNIDFMSLSSYPSASTKATGEVRVLLDVKNALHDKHVLIVEDI
                     IDT"
BASE COUNT          870 a          998 c         1000 g          856 t
ORIGIN      
        1 cgcaacgttg gaatacctgc agaagaatgg ccggatcggc ggggcagcta aactgatcgg
       61 cagcgcgctt cagatcaagc cgattctgca gatcgacgcc gggcacgtcg aagcgttcga
      121 aaaagttcgc acgcatcaca aagctctgga gcgaatccga gagatcgccc gtctggattg
      181 cccgcgcacg ctcgaatcgc acttgtgcgt aatgcacgcg gatgcgccca gcgaggcgaa
      241 tcagttgcgc gtggatttgt gtgccgacct gggcgtgtgc gatattccat tgtattcagt
      301 cgggccagcg ataaccacgc atgccggccc cggcacactt gcgatcggtt ttttcgtggc
      361 caactaaatt tccaacagcg atagtggacc ggcatgtctg aattaccaga gatcaaaaaa
      421 attgagcggg ccgttcgacg catgaccacg gtgcagttca gtgaactatg ctccgagctc
      481 ggcatgatcg aatcgctctt gggcgaggat ctaaacgaac ggaccaaaac actgatcggc
      541 ttgcaaaccg cgcccgataa cctaaaacat tttgtgcgca cgattcgcta tatctggccg
      601 gaagcgtttg acgagccggt aaccaaaccg aagcgcgtga tcaagatcga gccggggcaa
      661 atcatcggtg gcgtggcgct gctcatcatc ttgttcgtcg cggcgctcat gatcgcgagc
      721 gcgatcaatc cgccgagcgc gccgatcgcc gaattcaaag tcacaccgtc gatcattgcg
      781 acacacgtcg tgttagttgc ggtgcgcacg ccaacgccaa caccggtcac gccaactcgc
      841 accccggctc caaccgagac gcccgatgtg aatgcgacgt tgactgcgac ctttgcgcca
      901 tcgcccaccg agacgggtac ccccacccgc acgcccaagc cgaccagcca attcacagcg
      961 acaccgacgc cttcagccac acctgcggta caagcgattt atccacgtgt gcaattgcgc
     1021 aagcccgtga acaatgcaat cgtttcgatc aatatcaagc cgttagccca actgcaatgg
     1081 attgcacccg gcgaattaaa gccggatgaa cgctattggg tgcgcttccg ccggaatggc
     1141 agtgtcgctt atgcttattt cacttccaat aactggttcg atggtgtgcc caacaaccag
     1201 ctcggcgcgt atcaatggtc ggtggcgatt gtcaaagtgg atgagaacgg caatgtgctg
     1261 gggattctcg ggcctgaaag tgaagcgtgg aatattacgt ggcagtaaat ttcgatgctg
     1321 gaacttcgat attagcagcg gcgacttgat tatcaagtcg ccgtttttta atcttttcga
     1381 aattgtttct caaaccgatc gatctgctcg cgctgaacgg acgttggccg aataaacggt
     1441 cgcgctttcc gaatcgttgt ccacgctgct tgggcgctca gcccggttgt gatcagatat
     1501 gcggctgcta tcgatggtgc gcgcccgacg ccattcgcgc agtgaatata tacgccgtgt
     1561 cctgcggcga gctcatcccg gataaattct gccgcatgta ttaattcttc aaacgagggc
     1621 acgttatcat catcggtcgg gatcgaaaga tggtgttcga gatcgagact gtgctccaca
     1681 tcgccgtact cgcggcgcag atcgatgcag gccgtgatgt cacgcgcgcg catcgatttc
     1741 aagccgcgcc gctgatgctg tccgccgaca tatagatttt tcataacgcg gcttgtccgt
     1801 tcgggcgata cgccgacgag gatacgtgtc gtgcgctcgg tgaaccacaa acccgttgtg
     1861 cgcagacctt gatcgcgtaa tcggcccgca ataatgcgga tgcctttact gatcttgtgt
     1921 ggaatggaag tgggttgggt agaagatgtt gcttccatga aataaattga tcccgtagag
     1981 atgttccggt ggaacgtctt aagctgcctc accgtggcgt ctctacatta agattttctt
     2041 tcggtacatc ctatgcattt tataaaagtc tatgcctaag ctggcaatct cgcgctgcat
     2101 cttgtcgttg tccgcgccga tctgcactaa atcggcatgt tgaaagccgc cgtccttcac
     2161 gctcttatac atttccgaaa acagaatcgc cgtgccaccg ttgccgcgat attgctcgac
     2221 aatgcctgca ccgttgatgt tgacccactt ggtgcgcttg aattccagca gtagatcgat
     2281 ccagccgaac ggaaatagtt tgcctttcgt ccgctggatt gccgccgaaa catcgggata
     2341 ggcgaacaaa aacccgatcg gtcgatcgtc cttcatgacg atcttgatca aatgtggatc
     2401 ggcgtacgac agcaattgat cggccatcgt ctgtacttcg tcatcggtca acggcacatt
     2461 gccttccgtg ccgccgagcg agtcattata catctgcttc agcgggccaa tcaatgtacg
     2521 cagatcgcgg cgtgagttgt aacgcgcaat gtgcaggccg cgccgttttt gcaccagctc
     2581 ggagatgtga tgaatcttct ccggaaaatt caaatgactt ggatcaagat gccctgacac
     2641 cacatcatgc aagccttcaa aaccagaatc ttccaacagc cgcggataat aatcgggatt
     2701 gtagggaatg cctaaggcgg gccgatgctc aaaacctttc gccagcaacc ccatcccatc
     2761 gagcgcggta aaacctttcg ggccgaacac ttcatcgagt ttccggtcgc gcgcccagtc
     2821 gatcgccgcg gtgaacaagc cgcgtgaaat ttccacgtcg tcttcggctt caaacaagta
     2881 gaacagcgct tgatgcgtgt gattgaattc gttgtaatgc cgattctcta atacggcgat
     2941 ccggcccgcc actcgatcgt tcttcagcgc gagaaagaaa tccgcgtccg agtgttgata
     3001 gaacggatgg cgtctgcggt cgagcatgcg gcgcgcatcg gaatcaagcg gcggcaccca
     3061 ctgcggaata tttttataga gcgtgaatgg aaaatcgagg aagcgtttga cgtcgcggcg
     3121 gtcgttcgta tcgatcttga tgatgtccat gcggtcgatt gtagatcgat tcgagtgaaa
     3181 agtaaaaagt aattagtaac gagtaacgag taattaggaa ggcgtcacga gcatgcgcgt
     3241 cattcccgca tgttttaagc tggaatccag agttcatatc cgcgtaattc acttgccgtc
     3301 aaactggatg cccgacaaaa gcattcgggc atgacggctg aatagagttg tgcattactg
     3361 tgaatgcgct taaaatcatg tgcgactaaa actttagcga ggagttgttt atggattcag
     3421 ccattgaacg catcttgctt gacgaacgat ccattgcggc caaagtcaaa gaaatggccg
     3481 ggcaaatttc ggcggcgtat cagggcgtcg acgaattgat tgtcgtgtgc attctcaacg
     3541 gcgcgtttcg gttcacggct gatctagaag cgcatctgac cgtgccctgc aacatcgact
     3601 tcatgtcgtt gagcagttat ccatcggcct caacgaaagc gacaggcgaa gtgcgggtgc
     3661 tgttggacgt gaagaacgct ctgcacgata agcatgtgct gatcgttgag gacatcatcg
     3721 acac
//
LOCUS       DASRTJ010000679         1643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_369393,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000679 DASRTJ010000000
VERSION     DASRTJ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369393"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFF70_13760"
     CDS             <1..639
                     /locus_tag="VFF70_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZY45801.1"
                     /translation="EHGRFVNQLEVYALLALYFLEVRKERGAIVKTLSTTSMLEKLGK
                     LYDVPVIQTGVGFKYVAPKMIETHAMLGGEESGGFAFRGNVPERDGILGNLYMLDFMV
                     KTGKTQSQLLKYLFSKVGPHYYDRIDTRCTPEQRDAVWERAVNAKPATIGGLKFTSLD
                     TTDGFKFMMGDDGWMLIRLSGTEPIVRVYTETTHQDNVEAILQDGLKIAGLK"
     gene            641..1450
                     /locus_tag="VFF70_13765"
     CDS             641..1450
                     /locus_tag="VFF70_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZY45802.1"
                     /translation="MTNDQRQTTNDLVVGHSSLVDTHCHLDWNKFDPDRDQVIQRAIE
                     AGVTRMITVGVDISSSQRAIELAEKYPAVYAAIGVHPNDCSAFDKSMLDQVRSLARHE
                     KVVAIGEIGLDYYWQKTAKDQQARAFEMQLDLAAEVQKPVIIHNREATADVMKILPQH
                     HNIGVLHSFFESIEVAQQAIGLGYSIGFTGPITFRKAYRERAVAQEVPIDRILIETDA
                     PFLTPVPHRGQRNEPAYVKHIAEMIAQVTGLTFDEIARQTTSNAEKLFGLG"
     gene            1457..>1643
                     /locus_tag="VFF70_13770"
     CDS             1457..>1643
                     /locus_tag="VFF70_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase"
                     /protein_id="HZY45803.1"
                     /translation="MTTLTVLDPIIDDLRRVEAKLRETPQLKEIEFDLVSKAIDHLFS
                     SGGKRIRPSLALLTGRLY"
BASE COUNT          423 a          380 c          457 g          383 t
ORIGIN      
        1 gagcatggcc gcttcgttaa tcaactggaa gtttatgcgc tattagcttt gtatttcctt
       61 gaggttcgta aagaacgcgg cgcaatcgtc aagacgttga gcacgacctc gatgttggaa
      121 aaattgggga aattgtatga cgtgccggtg attcaaaccg gggtcggctt caaatacgtg
      181 gcgcccaaga tgatcgaaac acacgcgatg ctgggcggtg aagaatcggg cggctttgcc
      241 tttcgcggca atgtgccgga acgcgatggc atcctcggca acttatatat gctcgacttc
      301 atggtcaaga cgggcaagac gcaaagtcaa ctgcttaaat atctttttag caaagttggt
      361 ccgcactatt acgatcgcat cgatacgcgc tgcacgccgg agcagcgcga tgccgtatgg
      421 gagcgcgctg tgaacgccaa acctgcgacg atcggtggtt tgaagtttac cagcctcgat
      481 acgaccgatg ggttcaagtt catgatgggc gatgacggtt ggatgctgat tcgcttatcc
      541 ggcacggaac cgatcgtgcg ggtgtatacc gagacgacgc atcaagataa tgtggaggcg
      601 atcttgcaag atggcttgaa gattgctggc ttgaaataaa atgacgaatg accaacgaca
      661 aacgacgaat gatttggtcg ttggtcattc ttcgttggtc gacacacact gccatctcga
      721 ttggaacaaa ttcgatcctg atcgagatca agtgattcaa cgcgcgatcg aggcgggcgt
      781 cacacgaatg atcactgtcg gcgtagatat ttcatccagc caacgcgcga tcgagttggc
      841 ggaaaaatat ccagccgtgt acgccgcaat cggggtgcat ccgaatgatt gtagcgcttt
      901 tgataagagt atgcttgatc aagttcgatc gctcgcgcgc cacgaaaagg tagtcgcgat
      961 cggcgagatt ggtttggact attattggca gaaaaccgct aaggatcaac aggcgcgcgc
     1021 ttttgaaatg caattagact tggcggccga ggttcaaaag ccggtcatca ttcacaatcg
     1081 agaagcgacg gctgatgtaa tgaaaatatt gccccagcac cataacatcg gtgtgctgca
     1141 ttcgttcttt gaatcgatcg aagtcgcgca gcaagcgatc gggttgggat actcaatcgg
     1201 gtttaccgga ccgatcacgt ttaggaaagc ctatcgagaa cgcgcagttg cccaagaagt
     1261 tccgatcgat cgaatcttga tcgaaactga tgcgcctttc ttgacgccag tgccccatcg
     1321 gggccagcgc aatgagccgg cctatgtgaa gcatatcgcg gagatgattg cgcaagtaac
     1381 aggattgaca ttcgatgaaa ttgcgcgaca gacaacgtcg aatgcagaaa agttatttgg
     1441 tttaggatag atagttttga caacgttaac ggtactcgat cccatcatag atgacttgcg
     1501 gcgcgtggag gccaaactgc gcgagacgcc gcaattgaaa gaaattgaat tcgatctcgt
     1561 atcgaaggcg atcgatcatt tattcagcag cggtggcaaa cgcattcgcc cgtcgttggc
     1621 gctactgacc ggccgcttat atc
//
LOCUS       DASRTJ010000680         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_295130,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000680 DASRTJ010000000
VERSION     DASRTJ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295130"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..944)
                     /gene="rsmH"
                     /locus_tag="VFF70_13775"
     CDS             complement(27..944)
                     /gene="rsmH"
                     /locus_tag="VFF70_13775"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZY45804.1"
                     /translation="MNSIRHVTVLLQETIDSLDLQAGGHYIDGTLGSGGHSEAILIRA
                     AASEVLGLDADPAAIDRAAVRLGSFGSRVRFVNQNFSHLTEAAEAQNFANVDGIVFDL
                     GLSSDQLEDAQRGFGFLAGGPLDMRFDPTRGQSAAELVNTLDQGELADLIYRYGEEPA
                     SRKIARSIVAARPIHSAEQLADIIETTIGRRGRIHPATLTFQALRIAVNDELGSLMSV
                     LPQAVELLKRGGRLAIISFHSLEDRIVKEYFRSASQVHMAQPDDPPDLIRRKPTLKLM
                     TRKPIIPSVVEVENNPRARSAKLRVAEKV"
     gene            complement(958..1386)
                     /gene="mraZ"
                     /locus_tag="VFF70_13780"
     CDS             complement(958..1386)
                     /gene="mraZ"
                     /locus_tag="VFF70_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZY45805.1"
                     /translation="MFLGEYAHTIDDKGRLTLPAKFRTELAAGLVVTRGIDKCLFAFP
                     MDQWNKLAEQVSALPLTESQAREFRRLLFSGAADAELDKQGRVLLPQYLREYAGLDGD
                     VIVAGLNTHMEIWSPAAWNSTRANFELGALDAEHWAKLGI"
     gene            complement(1581..1950)
                     /gene="rnpB"
                     /locus_tag="VFF70_13785"
     ncRNA           complement(1581..1950)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFF70_13785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2174..3385
                     /locus_tag="VFF70_13790"
     CDS             2174..3385
                     /locus_tag="VFF70_13790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY45806.1"
                     /translation="MNNSARLTSSPLRRIFCISLLARLAHDTSIRMMYPFLPEIARGL
                     GLPIDQVSALVSLRNGVGIIGPAFGVMSDRIGHRRSMSIGLVCISIGLGLIGGLSALL
                     PAAIGFIIAGLGSVIYIPALQAYISERVPYEQRGRALGAIELTWAIAGMIGAPIAGGL
                     IGSIGWRAPFIGLAIAAFGCALLTLLLAETPQSLRSKVGRFKLSSLRQHRNAIAFLLV
                     WTLVFWAFENIQVGYGSWFETQFKLSATQRGTAQTLFGVFEIAASASSSLFLDRIGKK
                     RGVAGGLIVALIGYGLLMTIGPINLTLALGSMGLAFLGFEFSVVSGISIMSEQIPRAR
                     ATMLALGATGSGLGRMIGDLSGGALIANASFTTAALASAIMAALTLIIFVIGVQEKPF
                     SNEMAASDVPS"
BASE COUNT          862 a         1037 c         1007 g          937 t
ORIGIN      
        1 atctagatgg ttctaattga tatccatcag actttttccg caactctcag cttagcgctc
       61 cgtgccctgg ggttgttctc cacctcaacg acactgggaa taatcggctt gcgcgtcatc
      121 agtttcagcg tcggcttccg ccgaattaaa tcaggtggat catctggctg cgccatatgc
      181 acctgcgacg ccgatcgaaa atattccttg acgattctgt cttccaaaga atgaaacgaa
      241 atgatcgcta agcgaccgcc gcgctttaat aactcaactg cttgcggcag aactgacatc
      301 aatgaaccca actcatcatt cactgcaatc cgcagcgctt gaaacgtcag cgtcgccgga
      361 tgaattcgcc cgcgccgacc gatcgtggtc tcgataatat cggctagctg ttcggccgaa
      421 tggatcggtc gggcggccac aatcgatcgt gcaatcttgc ggctcgccgg ttcttcgccg
      481 tatcgataga ttagatccgc caactcgccc tgatcgagtg tgttcaccaa ctccgccgcc
      541 gattgccctc tcgtcggatc gaagcgcatg tccaacgggc cgccagccaa gaagccaaaa
      601 ccacgttggg catcctctaa ttgatcggat gacaacccaa gatcaaatac aattccatcg
      661 acgttggcaa agttctgcgc ctccgcggcc tcagtcaagt gagaaaaatt ttgattcacg
      721 aatctcactc gagatccgaa tgagcccaaa cggacagcgg cgcgatcgat cgcggccgga
      781 tcagcatcta gacctaaaac ctctgaagcc gcggctcgaa tcaaaatcgc ttcggagtgg
      841 ccgccgctgc ctaatgttcc atcgatgtaa tgcccgccag cctgtaggtc gaggctatcg
      901 atcgtctctt gtaaaagaac ggtgacatgg cgaatcgagt tcatgcttct cgattgatca
      961 gatgcccagc ttcgcccagt gttcagcatc cagcgcaccc aattcaaagt tcgcacgggt
     1021 ggagttccat gcagcgggcg accagatttc catgtgagta ttgagcccgg cgacaatcac
     1081 atcgccgtcg agaccagcat actctcgcaa atattgcgga agcaaaacac gaccttgctt
     1141 gtccaactct gcatcagcgg cgccggagaa caacaaccga cggaactcgc gtgcttggga
     1201 ctctgttaaa gggagagcgc tcacctgctc ggccaacttg ttccattgat ccatcgggaa
     1261 agcaaacagg cacttgtcga tgccgcgcgt cacaacaaga ccggcagcca attctgtccg
     1321 gaactttgcg ggcaaggtca agcggccttt atcatcaatg gtatgggcgt actcgcctaa
     1381 gaacatgtgg tctactctcc ccacctctgc cttgaagatg atcgaaaaga gggcaatgga
     1441 atcccacttt gccccacttt tccccacaag cactgacagt atacaccact ttttcctatt
     1501 ctgtctacta gcaatccaaa attttaaggt ttgggtttgt aaacaaaaac gcccatcgag
     1561 gaaatacctc gaaaggcgtt ttgtccaagg gatcgataag ccgaattctg tgcccggcaa
     1621 gccggatggc aatcatttct caagaccggc ggttacccga cggctccagc ggcctacccg
     1681 cgagattggg gacgagcagt ccttgcaccc ggaggcgcga ctcgcatgct tggccttgct
     1741 cccgatgggg gttgcctggc cggacgcgtt accgcttccg ccggtaggct cttaccctgc
     1801 cttttcaccc tgactcatta ctgagcggtc tgtttctgtg gtcctgctcc gtgggtcgcc
     1861 ccacccggat gttatccggc atcgcgctct atggagttcg gactttcctc gaacgagtct
     1921 agctcgctcg cgattgcctc gatctcttgg acactcttat cttagcactc ctaaataggc
     1981 gagtcaagat cagagctgta gagtctggct gtgtaaattg agtttagcat cactacaagt
     2041 aaatggaaca tgatcaaaat cgctgaggga gaaatccttt cggataggct gagaacttga
     2101 agatcaatcg aaccgaagat tatgggtatg atatacttca caaaacttct cgactccccg
     2161 ttaggttcat gtcttgaaca attcagcccg attaacttct tcaccgctcc gccgaatatt
     2221 ctgcatatcc ctactggcac gtctggcaca tgacacatcg atccggatga tgtatccgtt
     2281 cctgccggag atcgccagag gcctgggact gccgatcgat caagtcagcg cgctggtttc
     2341 cttacgcaat ggcgtgggaa ttatcgggcc agcgttcggc gtgatgtcgg atcgaattgg
     2401 tcatcgtcga tcgatgtcga tcggtttagt gtgcatctcg atcggcctcg gtttaattgg
     2461 cggcttgagc gcgttgttgc ctgcggcgat cggtttcatc atcgccggtt tgggatcggt
     2521 catttacatt cccgcattgc aagcctatat cagcgagcgc gtgccgtatg aacagcgcgg
     2581 tcgtgcgctg ggcgcgatcg agttgacgtg ggccatcgcc ggaatgatcg gcgcaccgat
     2641 cgccggcggg ttgatcggat cgatcggctg gcgggcgcca ttcatcggtt tagcgatcgc
     2701 cgcattcggg tgcgcactct tgactttgtt gttggcagaa acgccacaat cgctgcgcag
     2761 taaggtcggc cgattcaagt tatcctcttt gcggcagcat cgcaacgcga ttgcattttt
     2821 actggtatgg actttagtct tttgggcttt tgagaatatt caagtgggtt atggcagttg
     2881 gttcgaaacg caattcaaac tgtcagcgac ccagcggggc accgcgcaaa cactattcgg
     2941 cgttttcgag atcgcggctt cggcgagcag cagtctgttt ctggatcgaa tcggcaagaa
     3001 gcgcggggtc gcgggtggat tgatcgtggc gctgatcggc tatggactgc tgatgacgat
     3061 cggcccgatc aatcttacct tggcactcgg ctcaatgggc ttggcgttct taggctttga
     3121 gttcagtgtg gtctccggca tctcgatcat gagcgagcag attccacgag cacgcgctac
     3181 gatgttagcg ttaggcgcaa ccggcagcgg attgggtcgg atgatcggcg atttgagcgg
     3241 cggcgcactg atcgcaaacg ccagtttcac gacggcggca ctggcttcag cgattatggc
     3301 cgcgctgact ctgattattt ttgtcattgg cgtccaggaa aagccgtttt cgaatgaaat
     3361 ggcggcaagt gatgttccct catgaaggga cgtcgttcat ctttcattca caccgcgatg
     3421 ccatcgctac gattatgacg ttttagttcc gtcaaccctt tacaagaaca ttaaaaatca
     3481 attgtgaagc gctggacaaa acttattctt tcaggtatga ttgacacaga gcgcagcccc
     3541 aaggtgggct gtttgtttgt ctgctggttt gctgatcgac taagttctgg tatacttccg
     3601 cgcgtttggg tttggaagtt tgatgttgaa tattggaagt tagaatgatc agtcattcca
     3661 acatctaaca tccactttct aatcaccaac ttccaattag aggcactgag gaggcccaat
     3721 ggccagcaaa gagatggatg tgatgatcaa agacttgaca gccggactgc aaaagcagat
     3781 cgccgcattc acaccgaaga ctgaagccgt cgacgtcggt gaagtgcttg aagtcggcga
     3841 cgg
//
LOCUS       DASRTJ010000681         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_369941,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000681 DASRTJ010000000
VERSION     DASRTJ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369941"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="VFF70_13795"
     CDS             complement(<1..2225)
                     /locus_tag="VFF70_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45807.1"
                     /translation="MRSKTQVVLHLLVVTAMLLAAVGIIPASASFSSVQKNLAVLKAP
                     GPDDVALPPTARMIAMREAAIAACATDPTPAECVAEILGEREGDPLLRDEWFYDRRTA
                     GDPSVHFTMADAAALRAKAAEQAGTMMAQQALQPSAPNAFGGAWTAVGPNPMRLTGRG
                     DNSIDAMTGRIGALAVASTSPYTIYLGGAQGGVWESSWVTDTTTNQWTAKTDQLSSLA
                     IGAIALAPSNESIIYVGTGEGALSGDSYFGNGVLKSTDAGNTFSKISASGYFTNVSIS
                     KIVVANADPNTLYAGTLRGRGGARRTSPPDASPFGVWKSTNGGVNWTMVYTVTTDPLA
                     FAGVTDLEMDPQNSQVVYAAVLGDAIYKTVNGGTTWSPVMNGFPSTADYSVAPTRFAL
                     GISHPSAVVSATLYAGFEWYDTSFDRHPSAVWKSTNEGTSWSATSTSVIGDYCGSQCW
                     YDNLIGVDPISPTIVYALGLYDYGNGSGGIYRSMDGGANWTDLGFNLHPDYHAFAVRK
                     DAPANIVMGNDGGSWLSSSRGGRLLPSDPITATTWVNLNGKVNPATAGVLVRSGLQLG
                     QFESIATNPAVPDRFYGGTQDNGTLRKSDANTTWSDVASGDGGQVLVDPTDAKYMYGT
                     YYGLSPYRYVDGGGFYLGGFTANEYILNGLHNDRVEFYLPWIMDPGDSNRLYLGTYRV
                     YRTDNAKAPKSSDVLWNLISGDLTSGCTGSAANGGRGCTISALGAPAESPALYVGTEE
                     G"
     gene            complement(2619..3053)
                     /gene="queF"
                     /locus_tag="VFF70_13800"
     CDS             complement(2619..3053)
                     /gene="queF"
                     /locus_tag="VFF70_13800"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZY45808.1"
                     /translation="MSVDMHNEEMLSGLTLLGQKTNKPTRKLETFPNKHPGRSYVVTL
                     VCPEFTCLCAATGQPDFATITIKYIPDQRIVESKSLKMYLWSYRDEGHFHEHVTNLIL
                     DDLVKALDPIKCEVTGAFSVRGGIAITVEAKYERSGSVAQSG"
BASE COUNT          726 a          877 c          816 g          685 t
ORIGIN      
        1 cagccttctt ccgtgccaac atacaacgcc ggcgattcag ccggtgcgcc gagcgcactg
       61 atcgtgcagc cgcggccgcc attcgcggcc gacccggtac atcctgaggt caagtcgcca
      121 ctgatcaagt tccacaggac atcactggac ttgggggctt tggcattatc cgttcgatag
      181 acccgatagg tacccagata cagccgatta gagtcacccg gatccatgat ccatggcagg
      241 tagaactcaa cacggtcgtt gtgcaaaccg tttaagatgt attcgttggc ggtgaagccg
      301 ccgaggtaaa agccgccacc gtcgacatag cgatatgggc ttaagccata gtaagtcccg
      361 tacatgtact tcgcatcggt gggatcaacc aagacttgac cgccatcgcc gctggcgacg
      421 tcagaccaag tcgtattggc atcggacttg cgtagagttc cattgtcttg ggtgccacca
      481 tagaaacgat cgggcacagc tggattagtg gcaatactct cgaattgtcc gagttgtaaa
      541 cctgaacgaa caagaacacc ggcagtggcc gggttgacct tgccgttcaa gttgacccag
      601 gttgtcgcgg tgataggatc gctgggcaac aaacgtccac ctcggctgga tgataaccag
      661 gagccgccgt cattacccat gacaatgttc gcaggagcgt ctttgcgaac ggcgaacgca
      721 tggtagtccg ggtgcagatt gaagccgagg tccgtccaat tggccccacc atccatcgat
      781 cggtaaatac cgcccgatcc gttgccgtag tcatacagcc ccaacgcgta cacgatggtc
      841 ggcgagatcg gatcgacacc gatcagatta tcataccagc actgtgagcc gcagtaatcg
      901 ccaatgacag acgtgctcgt cgctgaccag cttgtgcctt cgttggtcga cttccagacc
      961 gctgagggat ggcgatcgaa cgatgtgtca taccattcga acccggcata cagcgtggcc
     1021 gagacgacgg ctgaaggatg gctgattccc agagcaaagc gagtgggtgc cactgaatag
     1081 tccgccgtgg acgggaagcc attcatcacc ggggaccagg tggttccgcc gttgactgtt
     1141 ttgtaaatag catcacctaa gacggccgca tagaccactt gcgaattctg cggatccatc
     1201 tctagatcag tcacacctgc aaacgctaag ggatcagtag tgaccgtata gaccatagtc
     1261 caattcacgc cgccgttagt ggatttccaa acaccaaacg gcgaagcatc aggtggcgag
     1321 gtgcggcgag cgccaccgcg tccgcgtagc gtgccggcgt agagcgtatt cggatcggca
     1381 ttggccacga cgatcttcga gatcgacacg ttggtgaagt aaccgctggc gctgatcttg
     1441 gagaaagtat tgccagcgtc tgtggacttc aacacgccat tgccgaaata actatcgccg
     1501 gataacgcgc cttcaccggt gccgacgtag ataatgcttt cattcgaagg cgctagcgcg
     1561 atcgcgccga tcgcgagtga actcaactga tcagtcttag cggtccactg gttagtcgtt
     1621 gtatcggtta cccacgaaga ctcccacacg ccgccttgtg cgccgccgag gtaaatggtg
     1681 tatggtgacg tcgaagccac ggccaacgcg ccgatgcgac cggtcatcgc atcgattgag
     1741 ttgtcaccac ggccggtcaa gcgcatcgga tttggaccca cagccgtcca cgcgccgcca
     1801 aaagcattgg gcgcactagg ttgaagagcc tgctgagcca tcatggtccc ggcttgttcg
     1861 gcggctttcg cgcgcagcgc ggctgcatcc gccatcgtaa agtgcacaga cggatcgcca
     1921 gcggtgcggc gatcatagaa ccactcatcg cgcaacagag gatcgccttc gcgttcgcct
     1981 aggatttcgg ctacacattc cgctggagtc ggatcggttg cgcaggctgc tattgctgct
     2041 tcgcgcatcg cgatcattct tgcggtcggg ggcaaggcca catcgtcagg gccgggcgcc
     2101 ttgagtaccg ctagattctt ttgaactgac gagaatgatg cgctggccgg gataatgccc
     2161 actgccgcca gcagcatcgc tgtcacaacc aacaaatgca atacaacttg tgtcttcgaa
     2221 cgcatggctt atattcctcc aagagatttg tgttttagat cgcatcaacc gagtgaatgg
     2281 tgaattcagg tgtgaagtgt tgtgcgcgag ccacctccct ttatcaaagc taataatcgc
     2341 aggcagttcc aatcaacaaa aaaacattta ccggcaccaa tctgccagca aatgttattg
     2401 atcttcagcg cgccaaacgc ccagaacttg tcgatgaatt taggctgaat cgatttacgg
     2461 ctagttccat aacggatgac agcgcctgac tcaaattgac cgcagtggac atgctccgag
     2521 gttgcatctt atttaatcac atccactgct agaagtcaat acattgatcg accggccagc
     2581 cttaaatttt tcgatgaaat gccgctccgg aacggtgctc accccgattg agccactgac
     2641 cccgatcgtt catacttggc ttccaccgtg atcgcgatgc cgccgcgcac gctgaaagcc
     2701 ccggtcactt cacatttaat cggatcgagc gccttgacca gatcatcaag tatcaaattc
     2761 gtgacatgtt cgtgaaaatg gccttcatcc cgatacgacc acagatacat cttcagcgac
     2821 ttcgattcga cgatgcgctg atcgggaata tacttgatcg tgatcgtcgc aaagtcaggc
     2881 tgaccggtcg ctgcgcacag acaggtgaat tcgggacaca ccagcgtcac cacatacgat
     2941 cgacccggat gcttgtttgg aaacgtctca agtttgcgag tcggcttatt cgtcttctgg
     3001 cctagcagag ttaacccgct caacatttct tcgttgtgca tatcaactga catttcaaca
     3061 tcctctcatt ctatttcaaa tttgaatacg gtgatcagat tatc
//
LOCUS       DASRTJ010000682         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_212057,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000682 DASRTJ010000000
VERSION     DASRTJ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212057"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFF70_13805"
     CDS             <1..283
                     /locus_tag="VFF70_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018795761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45809.1"
                     /translation="TDPSTIEVNEDRVLHWLSNGALPTESVEKLLTKVGTLDRFSRLK
                     KGESRETLLAEAKTAADALRTSATRQTRTNKATPSKKNRIKQTEAAPAA"
     gene            337..564
                     /locus_tag="VFF70_13810"
     CDS             337..564
                     /locus_tag="VFF70_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZY45810.1"
                     /translation="MKEFVEYVVKNMVDQPDQVVVKESHDGNLLVLDLHVAPDDMGRV
                     IGKGGRVANSIRSLLRVMATQADQRVTLEID"
     gene            609..1148
                     /gene="rimM"
                     /locus_tag="VFF70_13815"
     CDS             609..1148
                     /gene="rimM"
                     /locus_tag="VFF70_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZY45811.1"
                     /translation="MTHTRGSGSQKQLPEPHFLKVGMILRAWSIRGDVKVQPTAIQPE
                     ALQKFKRVFVGPHHREYKVQSFRAHQKNWLLKLEGVDTRNAAELLHGQWISIERQDQP
                     PLTEAEYYSNQIIGLRVETIDGAALGTVTEILKTGANDVYVVNGDRGEILLPAQAEVI
                     RSIDVENGIMIVKLLPGME"
     gene            1313..2425
                     /gene="dprA"
                     /locus_tag="VFF70_13820"
     CDS             1313..2425
                     /gene="dprA"
                     /locus_tag="VFF70_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZY45812.1"
                     /translation="MRDVRYWIAFNRVNGIGPAKVRALIDHFGDLERAWPATPIELKA
                     AGLDRKAIESLLQTRSAIDLDRELDRIEKIGAKIIIWDDPDYPPLLRNIPDPPPVLYM
                     KGQLTAADREWTIAIVGTRKASAYGRQAAETLAGELTRNGITIVSGLARGIDAAAHEA
                     ALSVGGRSIGVMACGIDQVYPPEHKRLADRLIEHGAVMTEMPIGSPPEAGTFPARNRI
                     ISGLALGTIVIEAGETSGALITSDRALEQGREVFAVPGNIFSKASLGTNRLIKEGATL
                     ITTAQDVMEALNLKMVVEHSAARAVIPDDATEAKLLSLLSHDPIHVDQIVNDSGLPIT
                     QVSSTLAMMELKGMVRQIGGMQYIVARESRAEYKVE"
     gene            2515..3594
                     /locus_tag="VFF70_13825"
     CDS             2515..3594
                     /locus_tag="VFF70_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HZY45813.1"
                     /translation="MDHYYALIMAGGVGTRLWPLSRQSRPKQVLPLVDERSMFKVAVD
                     RLQPLFDPENVFVVAGSDHIDLLRNEQCGIPVRNFIIEPMGRGTAPAIGLSAIHLQQR
                     DPQAVMAVLTADHFIGDEAGFRKVLEAAYQVAQADQLVTLGITPNSPSTGYGYIERGQ
                     KLIEVGGLTAYQVTAFREKPDLETARRFSADDQHSWNSGMFVWKVDRLLQELARSMPE
                     FYAQLLKIDQAWDTDRYEAVLTDLWPHVKSQTIDYGVLEQATDVAVIPAEFGWNDVGS
                     WATLLEILPSDANGNVVLHADSLGIDTTNTLVYGRDRLVATIGLHDFIVVDAGDALLI
                     CPKDRAQDVKKIVDQLKKEKKEQYL"
     gene            3638..>4255
                     /locus_tag="VFF70_13830"
     CDS             3638..>4255
                     /locus_tag="VFF70_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZY45814.1"
                     /translation="MEAYCVKCKTKREMTEAKPEYTASGTAATRGKCPVCGTTLYRMG
                     ATPEHANIPKPDPVAKSDKPRKKRASTKNGKGKNGSDAPRSGTLVIVESPAKAKTIGR
                     FLGKGYTVKPSVGHVRDLLRSTLSVDVEHDFTPKYRVPNEKKEVVKDIKKLAQKADEV
                     FLATDPDREGEAISWHLLEAAEIEPERAKRVVFHEITESAIKDAFS"
BASE COUNT         1043 a         1068 c         1256 g          888 t
ORIGIN      
        1 caccgatccg tccacgatcg aagtgaatga agatcgtgtg ctgcattggt tgagcaatgg
       61 cgcgttgccg actgaatcgg ttgagaagct gctgacgaaa gtcggcacac tcgatcggtt
      121 ttctcgctta aagaaaggcg aatcgcgcga aacactgttg gccgaagcga agacggccgc
      181 cgatgcgctg cgcacgagcg ccacgcgcca gactcgcact aataaagcca cacccagcaa
      241 gaagaataga atcaagcaga ccgaagccgc cccagcggcc tagtatcgtg agcggaacat
      301 tgaggtcgat gacttcaatg tggagtcgaa agggcgatga aagaatttgt cgagtacgtc
      361 gtcaagaaca tggtcgatca acccgatcag gttgtcgtga aggaaagcca cgacggtaac
      421 ctgctggtgt tggacctgca cgtcgcgccg gatgatatgg ggcgtgtcat cggcaaaggc
      481 ggccgcgtcg cgaattccat ccgatcgctc ctgcgcgtca tggccacaca ggctgaccaa
      541 cgggtaacgt tggagatcga ttgagcgagc gatcgatcga tccgcatcac tagtctcgag
      601 ctaatgcgat gacacacact cgtggctcag gcagccagaa acagctgcct gagcctcact
      661 ttttgaaagt cgggatgatc ctccgtgcct ggagcattcg cggcgatgtg aaagttcagc
      721 ccacagcgat tcagcccgaa gcgctccaga agttcaaacg cgtctttgtc ggtccacatc
      781 accgcgaata caaagtgcaa agtttccgag cgcatcaaaa gaattggctg ttgaaacttg
      841 aaggcgtcga tacacgcaat gctgcggagc tattgcacgg ccagtggatc tcgatcgagc
      901 gccaggatca accgccactc acggaggccg aatattattc gaatcaaatc atcggtctgc
      961 gtgttgaaac gatcgatggc gcagcattgg gcactgtgac tgaaatcttg aaaacaggcg
     1021 cgaacgatgt gtatgtggtc aacggcgatc gtggcgaaat tttgctgccc gctcaggcgg
     1081 aagtcattcg atcgatcgac gttgaaaatg gaattatgat cgtcaaattg ttgccgggta
     1141 tggagtaggg gcgaggcctt cgcacatcga tcgatgttga tctacgtgct catctggacg
     1201 cgattgctat tatccgccac cgcttgacaa atgcctcgcc cttaccagac aatcattctc
     1261 ttgacgattc acaacgtttc ggatatagtc gcgcctcgtc agaggtaacg tcatgcgtga
     1321 tgtgcgctac tggatcgctt tcaatcgagt gaacggcata ggcccggcta aagtccgcgc
     1381 gctgatcgat cacttcggcg atctggaacg cgcctggccc gcgacgccga tcgaactgaa
     1441 ggcagccggg cttgatcgaa aagcgatcga gagtttgctg cagacccgat cggcgatcga
     1501 tctcgatcga gaactcgatc gcatcgaaaa aatcggggcg aagatcatca tttgggatga
     1561 tcccgattat ccaccattgc tgcgtaacat tcccgacccg ccgcccgtct tatatatgaa
     1621 gggccaactc accgcggcgg atcgagaatg gacaatcgcg atcgtcggca cgcgcaaagc
     1681 cagcgcgtat ggccgccaag ccgcagaaac tttggcggga gagttgacgc gaaatggcat
     1741 cactatcgtc agcggactgg cgcgcggtat cgatgcggcg gcgcatgaag cggcattgag
     1801 cgtaggcggc cgatcgattg gcgtgatggc ctgcggaatc gatcaagtct atccgccgga
     1861 acacaaacgc ttggccgatc gattgatcga gcacggcgcg gtcatgaccg aaatgccgat
     1921 cggttcaccg cctgaagccg gaacctttcc ggcgcggaac cggatcatca gcggcctggc
     1981 cttgggcacg atcgtgatcg aagcgggcga aacgagcggc gcgttgatca ccagcgatcg
     2041 agcgttggag caggggcgcg aagtttttgc ggtgccaggc aatatttttt ccaaagccag
     2101 cctcggcacg aatcggttga ttaaagaagg cgcaacgttg atcactacgg cgcaagatgt
     2161 catggaagcg ctgaatttga aaatggtcgt cgagcattcg gcagcgcgcg cggtgattcc
     2221 cgatgatgcc accgaagcga aattattgtc gctattgtca cacgatccaa ttcatgtcga
     2281 tcagattgtg aatgacagcg gcctgccgat cacacaggtc agtagcacgc tggcgatgat
     2341 ggaactcaaa ggcatggtgc ggcagatcgg cgggatgcag tatattgttg cccgggagag
     2401 tcgcgcagag tataaagttg aataatgcgt actatgtact gcggaatact tctaactcaa
     2461 aaatagttac gcaatacgca ttacgtagta ttcttcgcgc attaagaatt gatcatggat
     2521 cattattacg cactgatcat ggctggtgga gttggcacac gactgtggcc gttgtcgcgc
     2581 cagtcgcgcc ctaaacaagt tctaccgctg gttgacgagc gatcgatgtt caaagttgcc
     2641 gtcgatcggt tgcagccgct cttcgatcct gaaaacgtat tcgtcgtcgc gggatcggac
     2701 catatcgatc tactgcgcaa cgaacagtgc ggcattcccg ttcggaactt tatcatcgaa
     2761 ccgatgggcc gcggcactgc tccagcgatt ggcttgagcg cgatccacct tcaacagcgc
     2821 gatccacaag cggtgatggc cgtgctgacc gcggatcatt ttatcggcga tgaagcggga
     2881 tttcgaaaag tgcttgaggc agcatatcaa gttgcacaag cggatcaatt ggtaacatta
     2941 ggcatcacgc cgaattctcc atcgaccggt tatggttata ttgagcgtgg ccagaagtta
     3001 atcgaggtgg gtggactcac ggcgtatcaa gtgacggctt ttcgtgaaaa gcccgatttg
     3061 gaaacagcgc gtcgtttcag cgcggacgat cagcatagtt ggaatagcgg catgttcgtc
     3121 tggaaggtcg atcggttact gcaagaactg gcgcgatcga tgccggaatt ttacgcgcag
     3181 ctgctgaaga tcgatcaagc gtgggacaca gatcgatatg aagcagtgtt gactgatttg
     3241 tggccgcacg ttaaatcaca gacgatcgat tatggtgtgc tggagcaggc tacagatgtc
     3301 gctgtcattc cggccgagtt cggctggaac gatgtgggtt catgggcgac gctgttagaa
     3361 attttaccga gtgacgcaaa tggcaatgtg gtgctgcatg ccgattcgct tggcatcgac
     3421 acgacgaaca cgctggtgta tggccgcgat cggctagtgg caacgatcgg gctgcacgat
     3481 tttatcgtgg ttgatgccgg tgatgcgctg ctgatctgcc cgaaggatcg agcgcaggat
     3541 gtgaagaaga tcgttgatca gttgaagaaa gaaaaaaaag aacaatatct gtagaggcga
     3601 ggtcgcctcg cccattctgg ataggtcata gaggaatatg gaagcatatt gcgttaaatg
     3661 taaaacgaaa agagaaatga ccgaggcgaa gccggagtat acggcttcag gcacggcggc
     3721 gacgcgcggc aagtgtccgg tgtgcggcac gacgttgtat cgcatgggcg cgacacccga
     3781 gcacgcgaat atccccaagc ctgatcccgt ggctaaatcc gacaagcccc gcaagaagcg
     3841 cgcttcaacg aaaaacggca aaggcaaaaa tggatcggac gctccgcgat ctggaacgct
     3901 agtgattgtg gaatcgccgg cgaaggcgaa gaccatcggt cggttccttg gcaagggcta
     3961 cacagtcaag ccgtcggtcg ggcacgtgcg cgatctgttg cgatcgactt tgtcggtgga
     4021 tgtcgagcat gatttcacgc cgaagtatcg cgtgccgaac gaaaagaagg aagtcgtcaa
     4081 ggatatcaag aaactggccc aaaaagccga tgaggtcttt cttgcgaccg atcctgaccg
     4141 cgaaggcgaa gccatttcgt ggcacttgct ggaagctgcc gagatcgagc ctgaacgggc
     4201 gaagcgcgtc gtatttcacg agatcactga atcggcgatc aaggatgcct tctct
//
LOCUS       DASRTJ010000683         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_370160,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000683 DASRTJ010000000
VERSION     DASRTJ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370160"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VFF70_13835"
     CDS             complement(<1..1404)
                     /locus_tag="VFF70_13835"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZY45815.1"
                     /translation="MRRIIPICFIIALIILAPIIPNFGDHLIYPAHSEFSDLTITHWP
                     AFAYARDEIVTTGQVPMWRTSILSGTPFAADPLAGLFYPPHWLALILPLPITFNLLML
                     LHLALAAASMYAMMRRWNVSRVGSIVSAIAYAAAPKVIAHMGVGHVTLVEAWAWLPVV
                     IAGLNPLNPDDIRNRLHAAQRPVIRGVGWRVGSGLALAMCLLADARIAIYAAALAVTY
                     LIVQHARRDRSTWLMIIMRVMIIVVVAVSVSAMSWLPTLSMTGGTARASITAEEAGTQ
                     SLDPIYFLGIVIADRSGAAERTTYFGIVILMLALVGVKLFYRRASRRVWWLIGVIIIG
                     AIVALGLNTPLYRVWLALPGMTLLRVPARAWFIVTFAIAALAGMGTQGLIEWSGQAKP
                     RSIFVAIIVAFAALVFGAIGALGSGQISFVAIALWIPIACLLLILRLKHKLSPDRFAI
                     LIVALIAIDLISIDWALY"
     gene            complement(1553..3106)
                     /locus_tag="VFF70_13840"
     CDS             complement(1553..3106)
                     /locus_tag="VFF70_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45816.1"
                     /translation="MLMPPRSLITSIERRWLLIASIVVLMLASLPYAFGALSSNSDWI
                     FTGLQVNPLDGVSYLAKMRIGFNGGWLFHLPFSIEQGPGAYLFTFFIALGHLARILNL
                     PLIVMFHLVRIIGGFSLLWLIYELIARFTEPIDRRRRVWWIVALSSGLGWLASLLGHT
                     QSSDLSIPESNTFYSLIANAHFALAAAIMISIFIGVLDAQKFSIKRVIGLSLLSLLLA
                     VIQPFAPFAVYAILGATLIAIWYRDRLFPWSKFFSTFIAGLATGPLLLYMYLATQADP
                     TLQQWSIQNQTPSPPLIDYAIGYGLLIAGAIIGIRSVWPRRSNWDILLIVWIVVAIPL
                     LYAPIPLQRRLSLGLHIPIAILAAYGIDRLIQAKWRKRLAIAATCLTSFFLLVVLIGG
                     AVKHDPHIYLSANEAAAFNWLAANAQPNDIILASPDLSLFVPAFTDQRVVYGHPYESV
                     NADRRKKQVTDFYSGQIDPATLLRADSVAYVLVGPRERALGQIDPAALPLNLVFTLGD
                     VSVYQVTLK"
     gene            3194..3895
                     /locus_tag="VFF70_13845"
     CDS             3194..3895
                     /locus_tag="VFF70_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45817.1"
                     /translation="MSKAETLLDLQAIDRVIDQLNKRLVEIQTALNESKKLTAARQAV
                     ANTEQLAAQNRSTHAELETENGTLAAKLDENEKRLYSGEVKVPKELIDLQNDVAMLKK
                     QKTALDDKVLAAMTKVDEADAALKDRRAELETIEAEWQANQIDFVADQTQLNSEVAQH
                     MQDQSKFRARLDTVDLALYDQLRRRKAGLAVVELEDGACNGCGVEPTADVIRLLNRGE
                     QIARCSNCERMLVQV"
     gene            4017..>5331
                     /locus_tag="VFF70_13850"
     CDS             4017..>5331
                     /locus_tag="VFF70_13850"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HZY45818.1"
                     /translation="MRFDFASFLIGVIIGCAISVVLYIQRESVARVWESFRAWLQRVR
                     NSITANIASRYQAALRLHLEQLSVPHAQADFDALYIDRFFDQPPARPTLNPIDPTRLM
                     PITLSAALRGSTRSAILGQSGSGRTTLLIKLARVLLDQQAQTQLGLSQEYMPLSVHLG
                     EIDWSKGTEADPLSALTEAAAAHMPKLIASNVNSFLKSCVRSNSAAVLLDGLDELSPG
                     LQSRAHEWLINLVKQFPNNQFAITTGLNNYGALNQIGFAALKLTDWTANDVEQLAQRW
                     IKVVSGGDQDFKVLSNSLRPIDAATPQPIDLTIAALVWKEHAAAPKDRSAAFAQWIDR
                     VLQAQPTKDRVAPDKIKSALGKIAWTTYQDNRIQVQTGEIEQAIFDTLALTSTQDRAA
                     LAGQVTEIAHAMANSSNLFIPFAADAWAFTHRHLAAYLAAWHAVQT"
BASE COUNT         1433 a         1461 c         1440 g          997 t
ORIGIN      
        1 atacaacgcc caatcaatcg agattaaatc gatcgcgatc agggcaacga tcaagatcgc
       61 aaatcgatcg ggcgacagtt tgtgcttaag ccgcaagatc agcaagaggc acgcgatcgg
      121 gatccatagc gcgattgcaa cgaaactaat ctggcctgaa cccagtgcgc cgatcgcacc
      181 aaataccaac gccgcaaacg caacgataat cgccacgaag atcgatcgag gtttagcttg
      241 gcccgaccat tcgatcaagc cctgtgtgcc catgcccgcc agcgccgcga tggcaaaggt
      301 gacaatgaac cacgcgcgcg ctggcacacg cagcaaggtc attccgggca aggctagcca
      361 gactcgatag agcggtgtat tcaagcccaa cgccacaatc gccccgatga tgatcacgcc
      421 gattaaccac cacacgcggc gtgatgcgcg tcgataaaat aatttcacgc cgaccagcgc
      481 cagcatcaag atcacaattc cgaaataagt cgtgcgttca gccgcgccgg atcgatcggc
      541 aatgacgatg cccaagaagt agatcggatc gagcgactgc gtgccggctt cttcggcggt
      601 gatcgatgcg cgcgccgtgc cgccagtcat agacagcgtg ggcagccatg acatagcgga
      661 tacggatacc gcaacaacga cgataatcat aacgcgcatg atgatcatca gccaagtcga
      721 tcgatcgcgg cgcgcatgtt gaacgatgag atatgtcaca gccaatgcag ccgcatagat
      781 cgcgatacgc gcatcggcga gtaagcacat ggccagcgcg agaccactcc ccacacgcca
      841 gcccactccc ctgatgactg gacgttgagc agcatgtaat cgattacgaa tgtcatcggg
      901 attgaggggg tttagaccag cgatcaccac tggcagccac gcccacgctt cgacgagtgt
      961 cacatgacca acgcccatat gcgcaatgac cttcggtgcg gcggcgtagg caatcgccga
     1021 cacgatcgat ccgacgcggc tgacgttcca acggcgcatc atggcgtaca tcgacgcggc
     1081 agcaagcgcg agatgcagaa gcatcaataa attgaatgtg atgggcaaag gtaaaatcaa
     1141 agcgagccaa tgcggtggat aaaacaggcc cgctaacgga tctgcggcga agggtgttcc
     1201 ggacaagatc gatgtgcgcc acatgggcac ttggccggta gtcacaattt catcacgcgc
     1261 gtaagcgaaa gccggccagt gcgtaatcgt cagatcgctg aattcgctat gcgcgggata
     1321 gatcagatga tcgccgaaat tcggaatgat cggtgcgagg atgatgagag caatgatgaa
     1381 gcagatagga atgattcggc gcatgattgt agttaaccga gcgtcattgc gaggagttct
     1441 cagcacgacg aagcaatctc cgaatcgaca ctgagacacc agcacttgca ccgcactacg
     1501 tgcggtgcaa gtgctgccaa gccgcctgcg gcggttcgca atgacaacaa ttttatttga
     1561 gtgtcacttg atacacactc acatcaccta atgtaaagac caaattcaat ggcagtgccg
     1621 ccggatcgat ttgtcccagc gcgcgttcgc gcgggccgac gagcacgtag gctactgaat
     1681 cagcgcgcag caaagttgct ggatcgattt ggcctgaata aaaatcggtt acttgtttct
     1741 tccttcggtc tgcgtttaca ctttcatacg gatgaccata aaccacacgt tgatcagtga
     1801 aagccggaac gaacaaactc agatcgggcg aagccaagat aatatcattc ggctgcgcgt
     1861 tcgcggccag ccaattaaag gcagcggctt cattcgctga gagatagatg tgcggatcgt
     1921 gtttgactgc accgccaatc agcacaacca atagaaagaa ggatgttaga caagttgcag
     1981 cgatagccaa gcgtttccgc cacttggctt gaatcaatcg atcgatgccg tatgccgcaa
     2041 gtatcgcaat gggaatatgc agaccgagtg acaatcgacg ctgcagcgga atgggcgcgt
     2101 acagcagagg aatcgccacg acgatccaaa cgatcaacag gatgtcccag ttcgatcggc
     2161 gcggccagac tgatcggatg ccgatgatcg cgccggcgat caataatcca tagccgatcg
     2221 cgtaatcgat caagggcggc gatggcgtct gattttggat cgaccattgc tgcaatgtag
     2281 gatcagcttg cgtggcgaga tacatataca agagcagcgg accagtcgct agaccggcga
     2341 tgaaggtgct gaagaacttc gaccacggaa ataatcgatc gcgataccag atggcaatta
     2401 acgtcgcgcc aaggatggcg taaaccgcga atggggcaaa gggctgaatc acggccaaga
     2461 gcaaagagag tagacttaag ccgatgacac gcttaattga aaatttctgc gcgtccaaca
     2521 cgccaatgaa gattgagatc atgatagccg ccgccagcgc gaagtgcgca ttggcgatca
     2581 aactataaaa cgtgttcgat tcagggatcg atagatcgga tgactgagtg tgccccagca
     2641 gtgatgccaa ccaacccaag cccgatgaca gcgcaacgat ccaccatact cgacggcgtc
     2701 gatcgatcgg ttcggtgaag cgcgcgatca attcatagat cagccacaat aagctgaagc
     2761 cgccgatgat gcgcacaaga tgaaacatga caatcaacgg cagattgagg attcgcgcga
     2821 gatgacccaa cgcgatgaag aaggtaaaga gataggcgcc cggcccctgc tcgatcgaga
     2881 acggcagatg aaaaagccag ccgccgttaa agccaatgcg cattttggct aagtaactga
     2941 cgccatccag cggattgacc tgtaagccgg tgaagatcca atcggagtta gagctgagcg
     3001 cgccgaacgc atacggcagc gaagcgagca ttaagacaac aatcgaggcg atcaacaacc
     3061 agcggcgctc aatggaggtg attaacgatc ggggcggcat caacatgttg aggattctag
     3121 catatcttgc ggttatgcat ttataaatca acacgctata atagaccata catcaatcat
     3181 tttggaggcg gccttgagca aagcagagac actgctcgac ctgcaagcga tcgatcgggt
     3241 gatcgatcaa ctcaacaaac ggctggtaga aattcaaaca gcgttgaacg aatctaaaaa
     3301 gttgacggcc gcgcgccagg ccgtggcgaa tacggagcaa ctcgccgcac agaatcgatc
     3361 gacacacgcc gaactcgaaa cggagaacgg gacgctggcg gccaagctcg acgagaacga
     3421 gaagcggttg tacagcggcg aggtcaaagt tcccaaagaa ctgatcgatc tgcaaaacga
     3481 tgtcgccatg ttgaagaagc agaaaaccgc gcttgacgac aaagtattgg cggcaatgac
     3541 caaagtcgat gaagctgacg ccgctttgaa agatcggcgg gctgagctgg aaacgatcga
     3601 ggctgaatgg caagcgaatc aaatagattt cgtcgccgac caaacccagc tcaacagcga
     3661 agtcgctcag cacatgcaag accaaagcaa gttccgcgca aggttggaca ccgtcgatct
     3721 tgcgctttat gatcaattac gccgacgcaa agctggacta gccgtcgttg agctggaaga
     3781 tggcgcgtgc aacggctgcg gcgtagaacc cacggctgat gtgatccgtt tactcaatcg
     3841 cggcgaacaa atcgcacgct gtagcaactg cgaaagaatg ttggtgcaag tctgaataac
     3901 agattacacg gatgacacag ataaaaatct aagtcagtgt aacgcctgca ctccaatgcc
     3961 cttcgacaat gcggtgcaag tgtccgtgac ctccgtggtt tttttcggac agcaagatgc
     4021 gctttgattt tgcctcattc ctcatcggcg tgattattgg gtgcgccatc tccgtagtgc
     4081 tctacattca gcgcgaatcg gtggcgcgcg tgtgggagtc cttccgcgcg tggttgcagc
     4141 gcgtgcgcaa ttcgatcacc gccaacatcg ccagccggta tcaagccgca ctgcgtttgc
     4201 atctcgagca attgagcgtg ccacacgctc aagccgattt cgatgcgctg tacatcgatc
     4261 ggtttttcga tcaaccaccg gcgcggccca cgctcaaccc gatcgatccc acccgcctca
     4321 tgccgatcac actcagcgcg gcgctgcgcg gctctacccg ctcggcgatc ctcggccaaa
     4381 gcggatcggg ccgtaccacg ctgttgatca aattagcgcg cgtgctgttg gatcaacaag
     4441 ctcaaacgca gctgggcttg tcacaagaat atatgccgct cagtgtgcat ctcggcgaga
     4501 tcgattggag caaaggcacc gaagcggatc cattgtcggc gctgacagag gccgctgccg
     4561 cgcacatgcc taaactcatc gcctctaacg ttaatagctt tctcaagagc tgtgtgcgat
     4621 caaactccgc cgcagtgctc ttagatgggc tggatgaact ttcacccgga ctgcaatcac
     4681 gcgcacatga atggctgatc aacctcgtca aacaattccc caataatcaa tttgcaatca
     4741 cgaccggcct caacaattac ggcgcgttga atcagatcgg tttcgccgcg ttgaaactca
     4801 ctgactggac agctaacgac gtcgaacaac tggcccagcg ctggatcaaa gtcgtcagcg
     4861 gcggcgatca agatttcaaa gtactgagta acagcctgcg tccgatcgat gcagctactc
     4921 ctcaaccgat cgatctcacc attgctgcac tggtgtggaa agagcatgcc gccgcgccca
     4981 aagatcgatc ggccgccttc gcgcagtgga tcgatcgggt gttgcaagcg cagcccacta
     5041 aagatcgcgt ggcaccggac aagatcaaaa gtgccttggg caagatcgcc tggaccactt
     5101 atcaagacaa tcggattcaa gtgcaaaccg gtgagatcga acaagcgatc tttgacacgt
     5161 tagccttgac ttcgacacaa gatcgagccg cactcgccgg tcaagtaact gaaatcgcgc
     5221 atgccatggc caacagcagc aacctgttca ttccattcgc cgccgacgcg tgggcgttta
     5281 cgcatcggca cctcgcggca tacctcgcag cgtggcacgc agtgcaaact c
//
LOCUS       DASRTJ010000684         7109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_370173,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000684 DASRTJ010000000
VERSION     DASRTJ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370173"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFF70_13855"
     CDS             <1..910
                     /locus_tag="VFF70_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45819.1"
                     /translation="SLIDGSYQLRARSIASGTISDTTPAAITFTLDTVAPLTPTVITP
                     NGGITIVSPAPIFVWSSGGNPTRFEINLDGITTSVNSPLTATQLNVSDGLHQWRVRAF
                     DAATNASGWSDIAQFHSTSFKSYLPMTLKEFAEVIPPDNNLTCTSVIPNGDFETGNWL
                     PEWQSLSQNPIPGVVTTNVYSGSTSARVGAAPGALAISTGFSSIQQLVAIPANALTAT
                     LSFAYNLYSGDTINDLQYVAVLSSGPIEFLIYQRSNDQTWRTSQFDLLPYAGQPIDLR
                     FSASNNTIGGSTGMLVDDVVLKVCTP"
     gene            939..2210
                     /locus_tag="VFF70_13860"
     CDS             939..2210
                     /locus_tag="VFF70_13860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZY45820.1"
                     /translation="MSTTFEPEKSDPLLGSTTLALRAVFERLSTEDISALRTVSHLKT
                     YPVNTILCHEGETEHTFYVLQAGTVKVTQRIPDGEDRLLAMRGPGEFFGEMAVIDNSP
                     RSASVTAASNVQVIEITQEVFDRVLSHSPELAITLLRHVMQYLRNTMQWQIVELQAKN
                     RALEAAYQQLQAAQAELVATERVKRDLEVAAHLQRSILPDHLPQPIGYHFAARAHPAR
                     EIGGDFYDVFKLDEQHLGILLADVSDKSIHAAIFMAVSRTLFLTEARRSLSPRQVVET
                     VNDLLLEISSSDDMFVTAFYGVLHLDDRKLTYVRAGQDKPLLQHADGTYEVLDGLGRF
                     IGMMPALIVEERTIDLQSGDRLILYSDGITDAMDATEQNYGVPRLQKTIWQHRTDSVE
                     KLADAIFSDVVSFEGGAPQFDDITLLVAAID"
     gene            2310..2801
                     /locus_tag="VFF70_13865"
     CDS             2310..2801
                     /locus_tag="VFF70_13865"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZY45821.1"
                     /translation="MTPEPIRRVEARAHQPNGGPLWIGPAIGLIVGLCGLIGAALFVS
                     FKPINQGSIAQPTALVITITPSPIAPTVTARPTLPPTATPEGLYVGGAAIVSGTGSSL
                     RLRSDPGLQATTLKTVLDGVQLTILEGPREADGVIWWRLHDPADGAEGWAAQDYLQPS
                     AGP"
     gene            2981..3481
                     /locus_tag="VFF70_13870"
     CDS             2981..3481
                     /locus_tag="VFF70_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZY45822.1"
                     /translation="MSQTSTSNEPKPVALRPRPGQTELTWREWLMLAGIVLFGLIFCA
                     IAGRSIINGFAASFPTPTATPPIVRPTVTLIPTATSAPTFTPTPNIPVSITLNLYVVV
                     VGGVNFRNDANTGAQIIRTLTDGEVLQVTGGPTAASGLNWWKLKDQNGTEGWAVQDYL
                     KPTAPP"
     gene            3501..5750
                     /locus_tag="VFF70_13875"
     CDS             3501..5750
                     /locus_tag="VFF70_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZY45823.1"
                     /translation="MTDPDKNGRNVNRIDAVAKVTGAALYPGDLVKENMLHMKILFAR
                     RPHAIIRRIDIVQAEQVPGVKAIFTAKDVPNNEYGLIIPDQPVLIGPGSNKQWGDRVR
                     FIGDQVALIVADSEKTAERARDLIVVEYEDLPILSDPREAIKDGAIQLHPDKANNILQ
                     HYRVRKGDVDSIWSKCNVIVESEYHTPFQEHAYLQPEAGLSYIDEEGRITVQVGGQWT
                     HEDQEQIAHALRLPLERVRVIYPAIGGAFGGREDMSVQIVLALATWKLQRPVKIVWSR
                     EESMIGHHKRHPMYLKAKWGARSDGKLLAAEIHVISDAGAYAYTSTKVQGNTTLTCTG
                     PYEIPNVKVDTYSVFTNNIPTGAFRGFGGPQGEFLAEMQMNKLADKLSIDPVELRLMN
                     VLREGSILSVGTPIPKGISMTPVLEKCALEVGWTKNLEIVESNLSMPSKILSQGWIRP
                     PRTSSTDPSKRIGLGFAIGYKNVGFSFGAPEQSTARIELHGTTEIEEAILYHASAEVG
                     QGTHTVMRQMAARALDMPIEKVQLIASDTATSGNSGSVSASRMTFMSGNSIKGAAELA
                     LKKWHEEERPAIAEYSWKAPKTTPFDPETGKSEPNFAYGYVAEAVEVEVDIETGHVRV
                     LRVWCADDVGRAINPELVRGQIEGGIVQAQGYTITENFQMKDGQVLTPYLSNYLIPTV
                     LDIPQKITSIILEYPDPLGPWGARGMAEMPYLPYAPAVCAAVKDAIGIWFDSFPLTPD
                     RVLKALKTK"
     gene            5811..6965
                     /locus_tag="VFF70_13880"
     CDS             5811..6965
                     /locus_tag="VFF70_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZY45824.1"
                     /translation="MSFANSLRDQFLLDPSIIYLNHGSFGATPRSVFEKYQAFQLELE
                     REPVEFLGRRFNDLMRAAREPLAAYLHVAADEIVYVSNATTGLNVVARSLDLKPGDEI
                     LSTNHEYGALDRTWRFLCGKAGATYINHQLPDPLESREQFIESMWSRVTDRTRVIFLS
                     HITSPTALLFPVEEICQRARSAGILTMIDGAHAPGQIDLDLQSIGSDFYSGTCHKWMC
                     APKGSGFLYARKDKQHLVEPLIVSWGYESDNPSGSKFIDHHEWLGTRDYAAYLATPSA
                     IEFMAQHDWPKVQSQCRILAREAQRLITEWSGRTVLNLDSYPHDLQMLTASLPPCDPV
                     STQGKLFAEYHIEIPFVAWDNRQWIRVSIQAYNSANDVDRLLIALRDILK"
BASE COUNT         1648 a         1840 c         1978 g         1643 t
ORIGIN      
        1 cagtttgatc gatggctcgt accagctgcg cgctcgatcg attgcgtctg gcacaatttc
       61 agatacgacg ccagcggcga tcacattcac gctcgatacg gttgcgccgt tgacgccaac
      121 ggtgattacg ccaaacggtg gtatcacaat tgtctcaccg gcgccgatat ttgtttggtc
      181 gagcggtggc aatcccactc gatttgaaat caatctggac ggcattacga cttcagtgaa
      241 tagcccgttg acagccacgc agttgaatgt gtcggatggg ctgcatcagt ggcgcgtgcg
      301 ggctttcgac gccgcgacga atgcatctgg ctggtcggac atagcccagt tccattcaac
      361 ttcgttcaaa tcatatttac caatgacctt aaaagagttt gcggaagtga tacccccaga
      421 caataacctg acttgcacga gtgtcatccc aaacggcgat tttgagacag gcaattggtt
      481 gccggaatgg caatcattat cacagaatcc aataccagga gtggtgacaa cgaatgtgta
      541 cagtggtagt acttctgcgc gcgtaggtgc tgcacccggt gctttagcta taagcacggg
      601 cttttcatca attcaacaat tggtcgcgat tccggcaaac gcgctaaccg ccacactttc
      661 gttcgcgtac aatttgtatt caggtgatac gatcaacgat ctgcaatacg ttgccgtatt
      721 gagtagcggg ccgatcgagt ttttaattta ccagcgatca aatgatcaga catggcggac
      781 aagccagttt gatctcctgc cctatgccgg ccagccgatc gatctgcgct tctcggcgtc
      841 gaataacacg attggcggtt caaccggcat gctagtggat gatgtcgtat tgaaggtgtg
      901 cacgccgtaa aattttcatt agctacagtt agattatcat gtcaacaacc ttcgaacctg
      961 agaaaagcga tccattatta ggttccacca ccctggcctt gcgcgcggtc ttcgagcgat
     1021 tgtcgacgga agatatctcc gcgctacgca ccgtgtcgca tctgaagact tatcccgtga
     1081 atacgatact gtgccacgaa ggtgagaccg agcatacttt ctacgtcttg caagcaggta
     1141 cggtcaaagt tacgcaacgc atcccggacg gcgaggatcg cttgttggcg atgcgcgggc
     1201 cgggcgaatt tttcggcgag atggccgtca tcgataactc gccacgcagc gcctcggtca
     1261 ctgctgcgag taatgttcaa gtcattgaaa ttactcagga agttttcgat cgagtcttga
     1321 gccacagtcc tgaactggcg attacactgc tgcgacatgt gatgcaatat ctgcgcaaca
     1381 cgatgcagtg gcaaatcgtg gaattgcaag cgaagaaccg cgcacttgaa gcggcttatc
     1441 aacagttgca agcggctcaa gccgaattgg tagcgaccga acgcgtgaag cgcgatttag
     1501 aagtggccgc gcatttgcag cggtcgatcc tgccggatca tctgccccag ccgattggtt
     1561 atcacttcgc cgcgcgcgct catccggcgc gcgagattgg cggcgatttc tatgatgtct
     1621 ttaagctgga cgaacagcat ctgggcattt tgctcgccga cgtttcagat aagagcattc
     1681 atgccgctat ctttatggca gtatcgcgca ccttgttttt aactgaagcg cgccgcagct
     1741 tatcgccccg ccaagtagtc gagaccgtca atgatttgct gttagagatt tcatcatccg
     1801 atgatatgtt tgtgactgcg ttttatggcg ttttacactt ggatgatcgt aaattgactt
     1861 atgtgcgagc cggtcaagac aaaccattgt tgcagcacgc cgatggtacg tatgaagtcc
     1921 ttgacggcct cggtagattt atcggcatga tgccggcctt aatcgtggag gagcgtacga
     1981 tcgatctgca gtccggcgat cgattgattc tctatagtga cggcattacc gatgctatgg
     2041 acgcgaccga gcaaaattat ggtgtgccgc gtttgcagaa gactatctgg caacatcgca
     2101 cagattcagt cgagaagttg gcggatgcga tctttagtga cgtagtgtcc tttgaaggtg
     2161 gtgcgccgca gtttgatgat atcaccttgc tggtggcagc aatcgattag ctcatcctcc
     2221 cgcaggttgt gctcaatgat cgtttgttgt ccaaataatt gtggcgctgt caaagttgat
     2281 cgatttgcct acaacctgcg ggaggttgca tgacaccgga acccattcgc cgcgtcgaag
     2341 cgcgcgcgca tcagccgaac ggcggtccgt tgtggattgg ccccgcgatc gggttgatcg
     2401 tcgggctatg tggtttgatc ggcgcagcgc tgttcgtaag cttcaaaccg atcaatcagg
     2461 gatcgatcgc gcagccgact gcgctagtga ttacgatcac gccatctccg attgctccaa
     2521 cggtgactgc gcggccaacg cttccgccga cggcgacgcc ggaaggttta tacgtgggcg
     2581 gcgcggcgat agtgagtgga accggatcat cgctgcgctt gcgatctgat ccaggtttgc
     2641 aagcgacgac gttgaagacg gtgctggatg gcgttcaact cactattcta gaaggtccac
     2701 gcgaagccga tggtgtaatc tggtggcgat tgcatgatcc agcggatgga gcggaagggt
     2761 gggcggcgca agattatttg cagccgagcg cgggtccata acaagcagga cctttgctca
     2821 taagcagttt atcctgtgtc accctgagcg aagcgaaggg tctcatacaa ttttgcgaga
     2881 tgcttcgtgg cacatcccta gagggctgtg ccatctaggg actgcgctca gtatgacaag
     2941 tgaaagtctc aacacgaaat catttgattg aggacagcca atgtcacaaa cttcgacgtc
     3001 caatgaaccc aagccggtcg cgttgcggcc acgacccggc caaactgaat tgacgtggcg
     3061 cgaatggctg atgctcgccg gcatcgtgct gttcggtttg atcttctgcg cgattgctgg
     3121 acgcagtatc atcaacggct ttgcagcttc attcccgacg cctacagcta caccgccgat
     3181 cgtacgtccg acggtgaccc ttatcccaac cgcgacctcg gccccgacgt ttacgccgac
     3241 gccaaatatt ccagtctcca ttaccttgaa tctatatgtc gtcgtggtcg gcggtgtgaa
     3301 ttttcgcaac gatgcaaaca caggcgcgca gatcattcgc acgttgactg acggcgaagt
     3361 actgcaagtg accggcggac cgactgcggc cagtggcttg aactggtgga agctcaaaga
     3421 tcagaacggc actgaaggct gggcagtcca agattatctc aagccgactg caccacccta
     3481 acagagccac aaggtgcaac atgactgatc ccgataagaa tggacgaaat gtaaatcgca
     3541 tcgatgcggt ggctaaagtg accggagccg ctttgtatcc cggcgatttg gttaaggaga
     3601 atatgctgca tatgaagatt ctctttgccc ggcgcccgca tgcgatcatc aggcggattg
     3661 atatcgtgca ggccgagcaa gtaccgggcg ttaaggcgat cttcaccgcc aaagatgtgc
     3721 cgaacaatga atacggtttg atcatccccg atcaaccggt gttgattggc cccggcagca
     3781 acaaacaatg gggcgatcga gtacgcttca ttggcgatca agtggcgctg atcgtggccg
     3841 actcagagaa gaccgccgag cgtgcacgcg atctgatcgt cgtcgagtac gaagacctgc
     3901 caatcttgtc cgatccgcgt gaagcgatca aagacggcgc gatccaactc catcccgata
     3961 aagccaacaa catcctccag cattatcgcg tgcgtaaagg cgatgtcgat tcgatctgga
     4021 gcaaatgtaa tgtgatcgtc gagagtgaat atcacacacc gtttcaagaa catgcttatc
     4081 ttcagcccga agccgggctg tcctatattg atgaggaagg ccggatcaca gttcaggtcg
     4141 gtggccagtg gacacatgag gatcaagagc agatcgcgca tgcgctgcgt ttgccgctcg
     4201 aacgggttcg agtgatctat ccggcgatcg gcggcgcgtt cggcggacgc gaagatatgt
     4261 cagtgcagat cgtcctagcg ttagccacat ggaaattgca gcggccggtc aagatcgtct
     4321 ggtcgcgcga agaatcgatg attggtcatc acaaacgcca tccgatgtac ctcaaagcga
     4381 agtggggcgc tcgatcggac ggcaagctgc ttgcggctga aattcatgtg atcagtgatg
     4441 cgggcgcgta tgcttacacc tcgacgaaag tgcagggcaa cacgacattg acttgcaccg
     4501 gcccgtatga aattcctaac gtcaaagtcg atacctattc cgttttcacc aacaacatcc
     4561 cgaccggcgc atttcgcggc ttcggtggtc cgcaaggcga gttcttagcc gagatgcaga
     4621 tgaataaact ggctgataaa ttgagcatcg atccggtcga gctgcgcttg atgaatgtcc
     4681 tgcgcgaagg atcgatcttg tcggtcggca cgccgattcc taagggcatc agcatgacac
     4741 cagtgctgga gaaatgtgcg cttgaagtgg gttggacaaa aaaccttgaa attgttgaaa
     4801 gtaatttatc catgccttcg aagatacttt cgcaaggctg gattcgtcca ccaaggactt
     4861 cttcaacgga tccgagcaaa cgcatcgggt tagggttcgc gatcggatat aagaatgtcg
     4921 gcttctcatt cggtgcgccg gaacaatcga ccgcgcgaat cgaactgcat ggcacgacgg
     4981 agatcgaaga agcgatcctg tatcatgcca gtgcggaagt ggggcagggc acacataccg
     5041 tgatgcggca aatggccgcg cgcgcgctgg acatgccgat cgaaaaggtg caactgatcg
     5101 catcggatac cgcgacgagt ggaaactccg gcagcgtctc ggcctcacgc atgactttca
     5161 tgtccggcaa ttcgatcaaa ggcgcggcag aattggcttt gaagaagtgg cacgaggaag
     5221 agcggcccgc catcgccgag tattcatgga aagcaccgaa gaccacgccg ttcgatccgg
     5281 agacgggcaa gagcgagcca aattttgcgt acggctacgt ggccgaagca gtcgaagtcg
     5341 aagttgatat tgaaaccggg catgtgcgtg tattgcgtgt gtggtgcgcg gatgatgttg
     5401 gtcgggccat caaccctgaa ttggtgcggg ggcagatcga aggcgggatc gttcaagcgc
     5461 aaggttatac catcaccgag aactttcaga tgaaagacgg gcaagtgctg acaccgtatt
     5521 tgagcaatta cttgatcccg accgtgctcg atattcccca aaagatcact tcgatcatcc
     5581 tggagtatcc tgatccgctg ggtccgtggg gtgcgcgcgg tatggccgag atgccctatc
     5641 tgccatatgc gcccgcggtg tgtgccgcag tgaaagacgc gatcggaatt tggttcgatt
     5701 catttccgct gacgcccgat cgggtgttga aggcgttgaa gacaaaatag gcgcagcgct
     5761 tagggtgcag acagcatacg cgctgcgtaa cacattcagt gaggttatgc ttgtcatttg
     5821 ccaactctct acgcgatcaa tttctgcttg acccgagtat catatacctc aaccacggat
     5881 cattcggtgc aactccccga tctgtttttg aaaaatatca agcgtttcaa ctcgaactcg
     5941 agcgtgagcc ggtggaattt ttgggccgcc gcttcaacga tctcatgcgt gctgcgcgtg
     6001 agccattagc tgcctacctc catgttgctg cggatgagat cgtctatgtc tcgaatgcga
     6061 caacaggctt aaacgtcgtc gcgcgctcgc tcgatctcaa acccggcgat gagatcctct
     6121 cgacgaatca cgaatacggc gcgctcgatc gcacctggcg ttttctgtgc ggcaaagctg
     6181 gtgcaactta catcaatcat caattacccg atccgctgga atcacgtgag caatttattg
     6241 aatcgatgtg gagccgcgtg accgatcgga cgcgggtgat cttcctgagt cacatcactt
     6301 cacccacggc gctccttttt cccgttgagg aaatttgcca gcgtgcccga tcggccggga
     6361 ttctgacgat gatcgatggc gcacacgcgc cggggcagat cgatctcgat ctacaatcga
     6421 ttggctcgga tttctattcc ggcacttgtc acaagtggat gtgcgcgccc aagggtagtg
     6481 gcttcttgta tgcgcgcaag gacaagcaac acttagttga gccattgatt gtgagctggg
     6541 gttacgaaag tgacaaccca agcggatcga agttcatcga tcatcacgaa tggctgggta
     6601 cacgcgatta tgccgcatat ttggcgacac ccagcgcgat cgagtttatg gcgcagcacg
     6661 actggccgaa agttcaaagt caatgccgca tattggcgcg tgaagcacag cggttgatca
     6721 ccgaatggag tggacgcact gtgttaaact tggattcata cccgcacgac ttgcagatgc
     6781 tgaccgcctc gctaccgccg tgcgatccag tcagcaccca gggcaagttg tttgcagaat
     6841 atcatatcga gataccgttc gttgcctggg acaatcgcca gtggatccgc gtttcgattc
     6901 aagcctataa ttcagccaac gacgtggatc gtttactgat tgcgctgcgc gatattttga
     6961 agtgaaagaa aagagatgaa taccaaacgc ttattgctgt ctgctctgat cggcctcccg
     7021 ttcatcagcg tgctggtggc gatcgatgcg tggctgtttc gggcaatact tggccttgat
     7081 tacctggcct ggtacctgca tagcggcac
//
LOCUS       DASRTJ010000685         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_370216,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000685 DASRTJ010000000
VERSION     DASRTJ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370216"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFF70_13885"
     CDS             <1..528
                     /locus_tag="VFF70_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZY45825.1"
                     /translation="EFQSYVDLLVKTGCIDNGKKIWWDVRPHPSFGTIEVRVCDICTK
                     VDEAIAIAALIQAIFVKIYWLFEKNMSFRLYNRSLINENKWRAARYGLDGELIDFGKQ
                     QSIPARQLLPELIEFVDEVVDDLGSRSAVEYALKMIEEGTSADRQLATYAKTNDLKSV
                     VDQIVEETLEGVVVA"
     gene            713..1069
                     /gene="rplS"
                     /locus_tag="VFF70_13890"
     CDS             713..1069
                     /gene="rplS"
                     /locus_tag="VFF70_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZY45826.1"
                     /translation="MNVMDTLVVKKADRPRLAPGDTVKVHTLIVEGDRERVQIFQGTV
                     IRSRKGGAQANFTVRRIASHGVGVERTFLLHSPRIEKIEVVRSTKIRRANLYYLRDLQ
                     GRAARLRERTTQTTEE"
     gene            1124..>1878
                     /locus_tag="VFF70_13895"
     CDS             1124..>1878
                     /locus_tag="VFF70_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZY45827.1"
                     /translation="MNYDPTLTQGARNAIQVCLRLQPNERLTLIADRAASEIAAALID
                     QIDQVGAEYRSFVLEDLIDRPSADMPIEILRDLERSQVSIFAAQAETGELSMRMQMMS
                     VVNRQRIRHGHMVNISKSIMLQGMRADFQQIDRLSSIIMGKARQAQRIKAQTPNGTDL
                     VAEFSPKLNWVKTSGIISRDKWGNLPGGEIFTSPFNVNGVFVVDGVVGDYLCQKYGDL
                     KSTPLRIEITDTRITHLDCANKDLLNEFAAYTRT"
BASE COUNT          425 a          490 c          548 g          415 t
ORIGIN      
        1 gagtttcaat catacgtcga tctactggtg aagaccggct gcatcgacaa cggcaaaaag
       61 atttggtggg acgtgcgccc tcatccgagt ttcggcacga tcgaggtgcg cgtgtgcgac
      121 atctgcacca aagttgatga agcgatcgcg atcgcggcgt tgattcaggc gatcttcgtc
      181 aagatttact ggctgttcga gaagaacatg tcgtttcgct tgtacaaccg atcgttgatc
      241 aacgagaaca aatggcgcgc cgctcgatat ggccttgacg gcgagttgat cgatttcggc
      301 aagcagcaat cgatcccggc gcggcagtta ctgccggagt tgatcgagtt cgtggatgaa
      361 gttgtggacg atctcggctc gcgttcagcg gtggagtatg cgttgaagat gatcgaggaa
      421 ggcaccagcg ccgatcggca gttggcgacg tatgcgaaga cgaatgattt gaaatcggtg
      481 gttgatcaga ttgtcgaaga gacgttggag ggtgtagtag tagcctaaac ccccacctag
      541 atatttgtgt tgatcgaagg tgatccttca tctcccccgg catgtcgggg agggttaggg
      601 gtgtggttga gagtaatttg agcggttcgc ttgtcagcct caatgaacca tgctataatc
      661 tgcttcgcgc tcgggtttcg agcgcattat tttgccagaa ggaactgttg tcatgaacgt
      721 catggatacc ctcgttgtca aaaaagccga tcgaccgcgt ttagcgccgg gcgatacggt
      781 taaagttcac accctgatcg tcgaaggcga tcgcgagcgc gtgcagattt tccagggcac
      841 ggtcattcgc agccgcaagg gcggcgcgca ggctaacttc accgtccgcc gtatcgcgtc
      901 gcacggcgtg ggtgtagagc gcaccttcct gctgcactcg ccacgcatcg aaaagatcga
      961 agtcgtgcgc agcaccaaga ttcgccgcgc caacttgtac tatctccgcg atctacaggg
     1021 ccgcgcggct cggctgcgtg agcgcaccac gcagaccacc gaagagtaaa tcggcatctc
     1081 acagagcgca ggccaaatcc tgcgctcttt ttattggaga aagatgaatt acgatcccac
     1141 tttgacccaa ggcgcgcgca acgccattca agtgtgtttg cgattgcagc cgaatgaacg
     1201 cttgacgctg atcgccgatc gagcggcgag cgagatcgcg gctgcgctga tcgatcagat
     1261 cgatcaagtg ggcgcggaat atcgatcgtt cgtgctggag gatctgatcg atcgtccctc
     1321 ggccgatatg ccgatcgaaa ttctgcgcga cctcgaacga tcgcaggtca gtatcttcgc
     1381 ggcgcaggcc gagaccggtg aattatcaat gcgcatgcag atgatgagcg tcgtcaatcg
     1441 ccagcgcatt cgacatggcc acatggtcaa catttccaag tcgattatgc tgcaaggcat
     1501 gcgcgcggac tttcaacaga tcgatcgatt aagcagcatc atcatgggca aggcgcgtca
     1561 agctcagcgc atcaaagcgc agacccctaa cggcacggac ctcgttgcgg aattttcacc
     1621 gaagttgaat tgggtcaaga ccagcggcat catctcgcgc gataagtggg gcaatctgcc
     1681 cggcggcgaa attttcacat cgccgttcaa cgtgaatggc gtcttcgtcg tcgatggtgt
     1741 tgtcggcgat tacctgtgcc agaagtatgg cgatctaaag tcgacgccgc tgcgtattga
     1801 gattaccgat acgcgcatca ctcacctcga ctgtgcgaac aaagatctgc tgaatgaatt
     1861 cgcggcttac acgcgcac
//
LOCUS       DASRTJ010000686         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_212108,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000686 DASRTJ010000000
VERSION     DASRTJ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212108"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFF70_13900"
     CDS             <1..462
                     /locus_tag="VFF70_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45828.1"
                     /translation="VNKWAIRLSISLLIAAAAVIVISLATSTNAVITVDWTTASELNT
                     AGFNLYRSEAQDGTYTRLNTDLIPGSTDPLTGGSYTFTDTNVIAGHTYYYQLEDLETS
                     GTATRHDPYEVKAGVNLGANLYVAIGLVTIAIILGALGLAGQPTSKTLIHD"
     gene            455..1261
                     /locus_tag="VFF70_13905"
     CDS             455..1261
                     /locus_tag="VFF70_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45829.1"
                     /translation="MTDLTWPQLSLSIAGLGVTIECNDQHVIDGLRDQWTAFNHHAPQ
                     IDLTARVQVDAAREPAAPDRPLIFRRDRVEFISSGYAGSIDAQHGVGSLSISNRQPND
                     DVRYFLRVVYALLAFRSGGLMLHAAGIVRSGRAYLFFGPSGSGKTTTVKYSPDHLVLN
                     DDLVILMPGSAQWEVFATPFWNEPQQRPNAQQHAPLAGLFRLVQSRSVFVEPMNGARA
                     AAEVLASTPITPLNSGYATTLLQRIEILVRKYPVHRLHFLPDASFWNVIE"
     gene            1529..2119
                     /locus_tag="VFF70_13910"
     CDS             1529..2119
                     /locus_tag="VFF70_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZY45830.1"
                     /translation="MVNSAALVVPSTDHHLLRQVAAGDEQAFDELYRAYNVIVYNYIL
                     RLMHEPNVAEDLLQEVFLAAWQGAARFRQESKVKTWLLRIAHYQTVSWLRRIKAVSSL
                     DDMLDLRDESEPIEEQIVQAWQAEQIRVALDQLSAKHRAVIELAFVHDLPYIEIADVL
                     ACPIGTVKSRISYALRSLTGGLKRMETRNETRVNDV"
     gene            2112..>2337
                     /locus_tag="VFF70_13915"
     CDS             2112..>2337
                     /locus_tag="VFF70_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45831.1"
                     /translation="MFKNLFRREIKTLLPGYINKTLSTTEQQAVDQQLRNDSAAQAEL
                     LAWQKIRSAVLARSQTTPSTLVRQRLLAQTR"
BASE COUNT          523 a          665 c          617 g          532 t
ORIGIN      
        1 gtgaataaat gggcgattcg actatccatc agcttgttga tcgcggcggc cgcggtcatc
       61 gtcatttcac tggccacctc gaccaacgcc gtcatcaccg tcgactggac aaccgccagc
      121 gaattgaaca ctgcgggttt caatttatat cgcagcgaag cgcaggacgg aacatacaca
      181 cgcctcaaca ccgaccttat ccccggctcg accgacccac ttacgggcgg ctcgtacact
      241 ttcaccgaca cgaacgtgat cgccgggcat acttactatt atcaattgga agatttagag
      301 acaagcggga cagcgacgcg tcacgatccc tatgaagtca aagccggcgt caacctgggc
      361 gcgaatttat atgttgcgat tggcttagtt actatcgcca tcatactggg tgcgcttggg
      421 ctggccggcc aaccaacctc gaaaacattg atacatgact gatctgactt ggccgcagtt
      481 atcactgtcg atcgccgggc tgggcgtgac gatcgagtgc aatgaccagc acgtgatcga
      541 cgggctgcgc gatcaatgga cggcattcaa tcaccatgcg ccacagatcg atttgactgc
      601 gcgggtgcag gtcgatgcag cccgtgagcc agctgcaccc gatcggccgc tgatcttccg
      661 aagggatcgg gttgagttta tctcttcagg ttatgccgga tcgatcgacg cgcagcatgg
      721 cgttggctcg ctatccatat ccaaccgaca acctaacgat gacgtccggt attttttacg
      781 cgtggtgtat gcgctgctgg cttttcgatc aggcggcttg atgctgcacg ccgccggcat
      841 cgtacgctca ggccgcgcgt acctattctt cggtccatcc ggcagcggca aaacgacaac
      901 ggtcaaatat tcaccggacc atctcgtgtt gaacgacgat ctggtcatct tgatgccggg
      961 gtctgcgcag tgggaagtat ttgccacacc attttggaat gagccccagc agcgcccgaa
     1021 cgcgcagcag catgcgccgc tggcaggatt attccggctc gtccaaagcc gatcggtttt
     1081 tgtggaaccg atgaacggcg cgcgtgcggc cgccgaagtc ttggcctcca caccgatcac
     1141 tccgctgaac tcgggttacg cgacgaccct gctacagcgc atcgagatcc tagtgcggaa
     1201 gtatcctgtc caccgattgc acttcttgcc tgacgcttcc ttctggaacg tgattgagta
     1261 gttcgattgt tgcgctcgca gccagtccgc tactgccctc cgccgtcggg tcttcgggaa
     1321 cattcaatca tcctgctttg acctggacga gtatcgctgc cgattcgaaa gtgaatctcg
     1381 atcaatactt catcggacgg atctattacc ggcggtcttg accagtcaag ctgacctcgt
     1441 ctatttgcca atcgtgttaa aatagaatct gcccaattga gtcacaagtc aattttgaac
     1501 ctattcttca cttatcacga ctaactatat ggtgaactct gcggcacttg tcgtacccag
     1561 taccgatcac cacctgctgc ggcaggtggc agccggcgac gagcaagcgt tcgacgaatt
     1621 atatcgagcc tataacgtca tcgtttataa ctacatcctg cgcttgatgc acgagccgaa
     1681 cgtcgccgag gatttgctgc aggaagtctt tctggcagcc tggcagggag cagcccgctt
     1741 tcgacaggaa tcaaaagtca agacttggct gctgcgcatc gctcattacc aaacggtctc
     1801 gtggttacga cggattaaag cggtatcgtc actcgacgat atgcttgacc tgcgcgatga
     1861 gagtgaaccg atcgaggaac agattgtgca ggcctggcaa gctgaacaaa tccgggtggc
     1921 gctcgatcag ctttcagcca agcatcgggc ggtgatcgaa ctcgccttcg tgcacgatct
     1981 gccgtatatc gaaattgccg atgtgctggc ttgtcccatc ggcaccgtga aaagtcgaat
     2041 cagttacgct ctgcgcagtc tcacgggtgg gttgaagcga atggaaaccc ggaacgaaac
     2101 gagagtgaat gatgtttaaa aatctttttc gtcgagaaat caaaactttg ctgccgggct
     2161 atatcaataa gacgttatcg acaactgagc agcaggctgt cgaccagcag ctgcgcaatg
     2221 actccgctgc gcaggctgaa cttttggcct ggcagaagat tcgatcggct gtccttgcac
     2281 gatcgcaaac taccccgtca acgctcgttc ggcagcgcct tctcgctcaa acgcgca
//
LOCUS       DASRTJ010000687         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_142479,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000687 DASRTJ010000000
VERSION     DASRTJ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142479"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2415
                     /locus_tag="VFF70_13920"
     CDS             <1..>2415
                     /locus_tag="VFF70_13920"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZY45832.1"
                     /translation="ATNKLSDYKVGLLPLEVKPVAQKLSIALTPDREKAQPGEEVTYQ
                     VHATSSDGQPVSAEFSLDLVDKAVLSLLPRAPDAIVEAFYGKRSLGVNTSSDLAVSVN
                     KFVEQLKQDLEAQPQARDAAVGQVIGGLGAGAENYAIAPSAAQPTPAAADGLALKDAA
                     GLPQNITVREQFADTAYWNPRISTDANGNASVKIKLPDNLTTWTFRGVGLTSDTKVGE
                     ATVDLISTKPLLVRPVTPRFFTVGDKAELAANISNNTDTALDVIAILSSTGVTLNSDA
                     SQTINIAAHSEGKVTWNVEVLDVRSVQLAFIAQAGDLIDASKPRLATAPDGSLIVYRY
                     TAPDIVGTAGQIKSAGGRTEIISLPPRFDVTKGEVSIQIDPSLAAGMINGLDYLEHYQ
                     YECTEQTVSRFLPNVLTYRALKDLGIDNKTLGDKLPSLVSEGLDRLYAGQHNDGGWGW
                     WIDDQSSPHLTAYVVFGLIQARDAGFDVKADAIDRGEQYLIQNLGSPARDFLNWDANR
                     QAWFLYVLSTDDRAPTNSIDDLFGERDKLSYYARAHLVMAIGQQNKEDARIQTILSDL
                     NNAAIVSATGAHWEEDSSDWWSMNTDTRSTAIILDAYAQLDKNNALAPNIVRWLMVAR
                     KIGYWETTQETAWALIGLTDWMRAKGELKPNYDYALFLNDKSISEGHLSPAQVQDPIK
                     LQIAVKDLIADVGNKLRFSRGDGAGVLYYTAHLKVYQPVEDLKPANRGVIVSRRYSLA
                     SCTETDRSKCPDVREVKLGDVIRVDLTIVASNDLYYLVVEDPLPAGAEAVDTGLATTS
                     MLAQGVS"
BASE COUNT          566 a          654 c          699 g          496 t
ORIGIN      
        1 gctacgaaca aactcagcga ttacaaagtc ggcctactgc cgctcgaagt gaaaccggtc
       61 gcgcagaaac tttcgatcgc gttaacgccc gatcgagaaa aggcgcagcc cggcgaagaa
      121 gtcacttatc aagtgcacgc cacctcgagc gatggtcagc cggtctcggc tgagttctca
      181 ctcgatctcg tcgataaagc agtattgtca ttactgccgc gcgcaccaga tgcgatcgtc
      241 gaggcgttct acggcaagcg ttcgttaggt gtgaacacgt ccagcgatct cgccgtctcg
      301 gtcaacaagt ttgttgagca actaaagcaa gaccttgagg cacaacctca agcgcgcgac
      361 gcagctgtcg gccaggttat cggcggtctc ggcgcgggcg ctgaaaacta tgcgattgca
      421 ccgtcggctg cgcagcccac accggctgcc gcggatggac ttgcacttaa ggacgcggct
      481 ggtttgccac aaaatatcac cgtgcgtgaa caatttgccg ataccgctta ttggaatcca
      541 cgcatcagca ccgatgcgaa tggcaatgca tccgtgaaga tcaaactgcc agacaacttg
      601 acgacgtgga cgtttagagg cgttggtctc accagtgata caaaagtcgg tgaagcgacc
      661 gttgatttga tttcaactaa acctttgctc gtgcgaccgg tcacgccgcg cttcttcacc
      721 gtcggcgata aagcggagtt ggcggcaaat atcagcaaca acacggacac tgcattggat
      781 gtcattgcga tcttgtcttc gactggcgtg acgttgaaca gcgacgcgtc gcagacaatc
      841 aacatcgccg cgcattcaga aggcaaagtg acgtggaacg ttgaagtgct cgatgtccga
      901 tcggtgcagc tggctttcat cgcgcaagct ggtgatctga tcgatgcctc taaaccccga
      961 ttggccacgg cgcccgatgg atcactgatc gtttatcgct atactgcgcc ggacattgtc
     1021 ggcacggcgg gacagatcaa atctgccgga ggccgcacgg agatcatttc gctgccgccg
     1081 cgcttcgatg tgaccaaagg cgaagtgagc atccagatcg atccatcgtt ggccgcggga
     1141 atgatcaacg gtctagatta tctggaacat tatcaatatg aatgcactga gcaaacggtc
     1201 agccgcttcc tgccgaacgt gttgacttat cgcgccttga aagatttggg gattgacaac
     1261 aaaactctcg gtgataagtt gccgagttta gtgagcgaag gacttgatcg attgtatgcg
     1321 ggccagcata acgatggcgg ctggggttgg tggatcgatg atcaaagcag cccacatctc
     1381 acggcgtatg tggtcttcgg tctgatccaa gcgcgcgatg caggcttcga tgttaaggcc
     1441 gatgcgatcg atcggggcga gcaatatctg atccagaacc taggcagccc ggcacgtgac
     1501 ttcttgaact gggacgcgaa tcggcaagcg tggttcttgt atgtcctatc gaccgacgat
     1561 cgagcgccga cgaattcgat cgatgatctg ttcggcgagc gcgacaagct gagctactac
     1621 gcgcgggcgc atctggtgat ggcgatcggc cagcaaaaca aagaagatgc gcgcatccag
     1681 acgatcctgt ccgatctgaa caacgcggcg atcgtcagtg cgactggcgc gcattgggaa
     1741 gaagacagca gcgattggtg gtcgatgaac accgacaccc gatcgacagc gatcattctc
     1801 gatgcgtacg cgcagctcga taagaacaat gcgctcgcgc cgaacatcgt gcgttggttg
     1861 atggtggcgc gcaagattgg ttactgggaa acgacgcagg aaacagcctg ggcgttgatc
     1921 ggtttgaccg attggatgcg cgcgaagggc gaactgaaac cgaactatga ctacgcgctc
     1981 ttcctgaacg acaagtcgat cagtgaaggc cacctctcgc ccgcacaagt ccaagacccg
     2041 atcaagttgc agatcgccgt gaaagattta atcgccgatg tcggcaacaa actgcgtttc
     2101 tcgcgcggcg atggcgccgg tgtgctgtat tacaccgcgc atctgaaagt gtatcagccc
     2161 gtcgaggatc tcaaaccggc caatcgaggc gtaatcgtat cccgacgtta ctcacttgcg
     2221 tcgtgcacgg agaccgatcg cagcaagtgc cctgatgtac gcgaagtgaa gttgggcgat
     2281 gtgatccgcg tcgatctgac gatcgttgca tcgaacgatc tatattacct cgtcgtcgaa
     2341 gatccgctgc ccgcgggcgc ggaagccgtc gataccggct tggcgacgac gagtatgctc
     2401 gcgcaaggcg tcagc
//
LOCUS       DASRTJ010000688         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_370434,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000688 DASRTJ010000000
VERSION     DASRTJ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370434"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="asnB"
                     /locus_tag="VFF70_13925"
     CDS             complement(<1..1207)
                     /gene="asnB"
                     /locus_tag="VFF70_13925"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZY45833.1"
                     /translation="MCGIVGCLSNPPIDLHVALDRIAHRGPDDEGIYIGAGVALGVRR
                     LSIIDLAGGHQPLSNEDGSVWIAYNGEVFNAPELRCELEKTGHRFRTHTDTETIVHAY
                     EHWGAEAIARLRGMFALAIWDAHQDRLVLARDRFGIKPLQYAEANGRFAFGSETRSIF
                     AALPELSPRLNHAALRTLFEIGFIPAPLTAFDQVYQLPAAHILIHEKNQTTIQPYWQL
                     EYPPIDQHRSIDLHAAAQEFVKQLHDAVDAWRMSDVPVGSLLSGGVDSASLAALLTEI
                     SGGQIHTFTIGFTAASHDESALARETARFLGSHHHELTFSAHDFDYLPNVVRHLEQPQ
                     CSATSVPIYLLYRACHAAGFKVIMTGEGADELLGGYHWFDGDRRARKFLRWPQFLRST
                     LAHAPINIST"
     gene            complement(1218..>1753)
                     /locus_tag="VFF70_13930"
     CDS             complement(1218..>1753)
                     /locus_tag="VFF70_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45834.1"
                     /translation="GGGSKMKLLLTVLIGVAALLVSCTAPATPAPTLAPSNVPPSLAP
                     TTRSTDAPTAAPAATNTALPPTAAPIDTQVPTAEPSPTLAAKPTADIVYMTFKDFEIV
                     PAEVTIKAGAKVVFLIKSDSKMFHQPYTFDQKDPFESPAGLGDGTSFAHTFNVAGTIT
                     IHCGYHDNMVAKVNVTP"
BASE COUNT          375 a          467 c          542 g          369 t
ORIGIN      
        1 cagttgagat attgatcggc gcatgcgcga gcgtgcttcg caagaattga ggccagcgca
       61 gaaattttcg cgcgcgtcga tcgccgtcga accagtgata gccgcccagt aattcatccg
      121 cgccttcgcc ggtcatgatc actttgaagc cggctgcgtg gcaagcacga tagagcaaat
      181 agatcgggac gcttgtggcc gaacactgcg gctgctcaag atgtcgaacg acattgggca
      241 agtaatcgaa gtcatgcgcg gaaaaagtga gttcgtgatg atgactgccg aggaagcgcg
      301 ccgtctcacg cgccagcgcc gattcatcat gcgacgcggc ggtgaagccg atggtgaagg
      361 tatgaatctg tccgccggaa atttcggtga gcagggctgc gagtgaggcc gaatcgacgc
      421 cgccgctcaa aagtgatccg accggcacat cgctcatacg ccacgcgtcg acggcatcat
      481 gcagctgctt cacaaattct tgcgcggctg catgcagatc gatcgagcga tgctgatcga
      541 tcggtggata ctctaattgc cagtaaggct gaatggtcgt ttggtttttt tcgtggatca
      601 aaatgtgcgc ggcgggtagt tgataaactt gatcgaacgc agtcagcggc gccgggataa
      661 atccgatctc gaagagggtg cgcagcgccg cgtgattgag acgcggggac aactccggca
      721 gggccgcgaa gatcgatcgg gtttccgagc caaacgcaaa gcggccgttg gcttcggcgt
      781 actgcagcgg cttgatcccg aatcgatcgc gcgccagcac caatcgatct tgatgcgcgt
      841 cccagatcgc cagcgcaaac atgccgcgca gccgggcaat ggcctccgcg ccccagtgtt
      901 cgtaggcatg cacgatcgtt tcagtgtcgg tgtgagttct gaaacggtgt ccggtttttt
      961 caagctcgca gcgcagctcg ggcgcgttaa agacctcgcc gttgtaagcg atccacacgc
     1021 tgccgtcttc gttgcttaac ggttggtgcc cgcccgcgag atcgatgatg cttaaacggc
     1081 gcacacccaa cgcgacgcca gccccgatgt agatgccttc atcatcggga ccgcgatgcg
     1141 caatgcgatc aagcgcaacg tgaagatcga tcggaggatt agataaacaa ccgacaatgc
     1201 cgcacatgga tgatgagcta tggcgtgacg tttactttgg cgaccatgtt gtcatgatag
     1261 ccgcagtgaa tggtgatagt gcctgcgaca ttgaacgtgt gcgcgaaact ggtgccgtcg
     1321 cccaaaccgg ccggcgactc gaatggatct ttttgatcga aggtgtaggg ctgatgaaac
     1381 atcttcgagt cactcttgat cagaaacacc actttggcgc cggctttgat cgtgacttcc
     1441 gctgggacga tttcaaaatc tttaaaggtc atgtatacga tgtcggcggt tggcttcgcg
     1501 gcgagtgtgg gactaggctc ggccgtcggt acttgtgtat cgatcggcgc ggcagttggc
     1561 ggcagtgcgg tgttggtcgc cgctggcgcg gcggtcggcg catccgtcga tcgcgtggtc
     1621 ggagccaatg atggcggtac atttgacggc gccaatgttg gcgcgggcgt ggcgggtgca
     1681 gtacagctga ccaacaaggc agccacgcca atcaaaacag tcagtaacaa tttcattttt
     1741 gagcctcctc caa
//
LOCUS       DASRTJ010000689         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_142830,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000689 DASRTJ010000000
VERSION     DASRTJ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142830"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFF70_13935"
     CDS             <1..623
                     /locus_tag="VFF70_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZY45835.1"
                     /translation="AKLYGVRDVPGQVEKYLRMLGLWDRRKDPAGSFSKGMKQKLAIA
                     RAILHEPATLFLDEPTSALDPESAKVVRDFIEELKTEGRTIFLCTHNLDEADRLCDRI
                     GVFKQHLIKVDSPSRMRTALFGRSIEFRLHKIDDSTAQLIKGFDFVKHVEIQSDKLIV
                     GLDNPEEGNPQIVKALVEAGAEVQFVSEIRHSLEEVYFSLINNGNK"
     gene            632..1417
                     /locus_tag="VFF70_13940"
     CDS             632..1417
                     /locus_tag="VFF70_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY45836.1"
                     /translation="MRIRTIINKEWSEVFKNRMVLMTVGLLPLLFTAIPLIQLAVTRT
                     FDPGNMNSMPTSLALQCHLEQLSDTECLQSFLSNQFMLFFLLMPLAIPVIIASYSIVG
                     EKITRSLEPLLATPISTSEIILGKALAASIPAIIATWGSFVIFLVVARFLVVSDRVYA
                     GIVNPMWLVAIFLVAPLMTVLSVNVAIIISSRVSDPRTAEQLGMVVMIPLLGIFFAQI
                     FGLIALNVTSMFLMAIAVLMLDFVLVGIGVKLFQREAILTRWK"
     gene            1533..2093
                     /locus_tag="VFF70_13945"
     CDS             1533..2093
                     /locus_tag="VFF70_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45837.1"
                     /translation="MQKPSPLARIWNALKNINLIISMFVNLILIVVVFVLITQIGAIK
                     ATLSTVLGQLDSAFEGLGSSIIADTIAIDQHVPIQFDLPIDQSITVETQAPIPLSVPT
                     TFSLGAFGTINGTVSLEIPSGTSLPVRLRLTVPVNTQIPVKFDQPISINLYSKGLGPV
                     VNKLRAALAPLIKLVHQLPDGFMLIK"
     gene            2140..>2781
                     /locus_tag="VFF70_13950"
     CDS             2140..>2781
                     /locus_tag="VFF70_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY45838.1"
                     /translation="MTDLRSLCAQHLPDILSLTKQIAALESPSTDKSALDACAAFVAQ
                     QLRSIGAQVQSVPQISAGNHLFATWGGTVQSDRPFLSLLHIDTVWPIGTLTDMSIRED
                     AGKLYGPGIYDMKASAAMTLVALRILQESGLKPRHEIHMLFTSDEEIGSETSRELIEA
                     EAKKSVLVLVMEPAMGGGQLKTFRKGVGTFNVVAYGRAAHAGVDHQKGVNAIEE"
BASE COUNT          672 a          708 c          687 g          714 t
ORIGIN      
        1 tcgctaagct atatggcgtg cgcgatgttc cgggccaagt cgaaaaatac ttgcggatgc
       61 tgggcttatg ggatcgacgc aaggatccgg ccggatcatt ttcgaaaggc atgaagcaga
      121 agttggcgat tgcgcgcgcg atcttgcacg aaccggcgac tctgtttttg gatgaaccaa
      181 cctccgcgct cgatcccgaa tcggcgaaag tggtgcgcga ttttatcgaa gaattgaaga
      241 ccgaaggccg gacgatcttt ttatgcacgc acaatctcga cgaagccgat cggctgtgcg
      301 atcgaattgg cgtgttcaag cagcacttga tcaaagtcga ttcacccagc cggatgcgga
      361 cagcgttgtt cggccgctcg atcgagtttc gcttgcacaa gatcgatgat tcaacggcgc
      421 agctgatcaa aggctttgac ttcgtcaagc atgttgaaat ccaatcggac aaattgatcg
      481 ttgggctgga caatccagaa gaaggcaacc ctcaaatcgt aaaggctcta gtcgaggcgg
      541 gagccgaggt ccaattcgtg agtgaaatac gacactcgct ggaagaagtt tatttttccc
      601 tcatcaacaa tggaaataag taggaggcag catgcgtatt cgcaccatca tcaacaaaga
      661 atggtcggaa gttttcaaga atcggatggt cctgatgacg gtcggcttgc tgccgctgtt
      721 attcaccgcc ataccgctca tccaattagc ggtaacccgc accttcgatc ccggcaatat
      781 gaacagcatg ccgacgagcc tcgcgctaca atgccatttg gaacaactca gcgacacgga
      841 atgtctgcaa agtttcttgt ccaaccagtt catgttgttc tttctcttga tgcccttggc
      901 gatccccgtg attatcgcct cgtacagcat tgtcggcgaa aaaatcaccc gatcgcttga
      961 accactgtta gcaacgccaa tctctaccag tgagatcata ctcggtaaag cgctcgccgc
     1021 atcaattcca gcgatcatcg ctacttgggg gtcgttcgtt atctttttgg tcgttgctcg
     1081 tttcttggtc gtaagcgatc gtgtatatgc gggtatcgtc aatcccatgt ggttggtagc
     1141 aattttctta gttgcgccgc ttatgactgt actgtccgtc aatgtcgcga ttatcatctc
     1201 atcgcgtgtc agcgatccac gcaccgctga gcagttaggt atggttgtca tgattccgtt
     1261 gcttggaatt ttcttcgctc agatttttgg cctaattgca cttaacgtta cgagtatgtt
     1321 cctgatggca atcgctgtgt taatgctaga ttttgtatta gtcggcattg gagtcaagtt
     1381 attccaacgc gaagcgattc tgacgcgttg gaaatagtca ccgaacacac aacgtggctc
     1441 ataaacaaat ggcgagattt tatttgcttt atccatttgt aaatgcttgg atacaagccg
     1501 tggattaatt tcgatcattt aggagacgcc atatgcagaa gccgtcgccg ctcgcccgaa
     1561 tttggaatgc acttaaaaat attaatctga tcatatcgat gttcgtcaat ttgatattga
     1621 tcgtagttgt atttgtcctc atcacacaaa tcggggcgat aaaggctaca ttgagcactg
     1681 tacttggtca attagattct gcgtttgaag gattaggcag ttcaatcatc gccgacacaa
     1741 tcgcgatcga tcagcatgtg cccatccaat ttgacttgcc catcgatcaa agcattacgg
     1801 ttgaaacgca agcacccatc cccttgagcg tgcccaccac cttctcgctt ggcgcattcg
     1861 ggaccatcaa cggcacagtc agcctggaaa taccttcagg tacgtcgctt cccgtacgcc
     1921 ttcgactcac tgtcccagtc aacacccaga ttccggtcaa gttcgatcag cccatatcga
     1981 tcaatctgta ctcgaagggc ctagggcccg ttgtgaacaa attgcgcgct gctttagcgc
     2041 cactcattaa gttggtccat cagctgcccg atgggtttat gctgatcaaa tagccgattg
     2101 ccgacgtttc acatgaaacg ttcgccttcc gaggaactcg tgaccgacct tcgatcattg
     2161 tgcgcccaac atttgcccga tatactgagt ctcacaaagc agatcgccgc gctggaatca
     2221 ccttcaacag acaaatctgc tctcgatgcc tgtgcggcct ttgttgccca gcaactgcgc
     2281 tcgatcggcg cgcaagtcca atcggttccg cagatatccg ccggaaacca cctcttcgcg
     2341 acttggggtg gtaccgtgca gagcgatcgc ccgttcttgt ctttattgca catcgacacc
     2401 gtctggccaa taggcacact caccgatatg tcaattcgcg aggacgccgg aaaactatat
     2461 ggcccaggca tctatgacat gaaagctagt gccgctatga ctttggtggc tttgcgaatt
     2521 cttcaagaat ctggactcaa gccacgtcac gagattcata tgttgtttac gagcgatgaa
     2581 gagatcggca gcgagacatc tcgcgagttg attgaggctg aagcaaagaa gagtgtgcta
     2641 gtgctagtaa tggaacctgc gatgggtggc ggtcaattga aaacttttcg caaaggtgta
     2701 ggtacgttca acgtcgtggc ctatggacgc gcagctcacg caggcgtcga tcaccagaaa
     2761 ggcgtgaacg cgattgagga g
//
LOCUS       DASRTJ010000690         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_142872,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000690 DASRTJ010000000
VERSION     DASRTJ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142872"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFF70_13955"
     CDS             complement(<1..1062)
                     /locus_tag="VFF70_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45839.1"
                     /translation="MNTRLYKFSVVISSIMLMLILSACASPIVTPTHTIAPSVVPTKT
                     LAPTPAPKPIAIYSQGSLPEAVRSHLDEMITAQPAQFTLAGDPATASLQVGYTAHADV
                     PVIGQWVFAFVAPFPTAIDDVPWSAVQSAWQGQPVTCEGCTFTGTLKLTDSTLQALRS
                     ILGDPAPNAIEVVPTDQLTQKLWAIQPALAIVPFDELNVRMKVLSIDGLTPLHRNLDL
                     NTYPLIVNVGLAGSIGDAQAAQAAFGQSITNRDESKLTIVAMTGVTAMSRDFAASMDT
                     NGVLYPDTLIKDYFTSSDIVHISNEVSFDPDCPKQPTLNRGIFCSDPKYMDLLTDVNV
                     KIIELTGNHLNDYGWQPFST"
     gene            1154..2632
                     /locus_tag="VFF70_13960"
     CDS             1154..2632
                     /locus_tag="VFF70_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45840.1"
                     /translation="MTVEVQAMRRVSTSIIVPYLISIALIFLITGAFYLLNIENDTPI
                     VALLYLLPVGLSTALWGLGPGIACAVSAFFVFNYYFIQPTYTLTVHQPQDVLALIVFL
                     VVAIVISQLVGRAKLNLATANTREHEASQLYDLGTLLSASHDDGSIARGLAYKVSQTF
                     QADRVGVSLETRLASQALLIVEPHTSTSPIGKPTIIVPLMTARGLQGEIRVWRKEPLS
                     MGEERLLQTFATQGGLSLDRSRLAQAETRAKVLEESDRLKSALLSSVSHELRTPLATI
                     KASISSLRSETVDWDSAARHDLLAAIEEETDYLNQLVGNLLDMSRIEAGALNPQKKWN
                     SLAEIVGSVLSRMRSAAQEHRLEIDLSDDLPLVLVDYVQLAQVFSNLISNSLKYAPIQ
                     STIKISARVKPDRSIQIEVINQGPPVPAEHLDRIFDKFYRVTAADRVTGTGLGLSICK
                     GMIEAHGGRIWAENRPEGFAFVFMLPAESVNQPKMPIEKEAS"
     gene            2629..>3114
                     /locus_tag="VFF70_13965"
     CDS             2629..>3114
                     /locus_tag="VFF70_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45841.1"
                     /translation="MTNPPRILVIDDEPQILRAMRTILTAKQFEVITASRGEEGLALA
                     AANPPDVIVLDLSLPDMDGIQVCARLREWTQIPIIVLSVRDNERDKVAALDKGADDYL
                     TKPFGIEELLARIRVALRHSVHSQGSKKTIVNAGTLSIDLANHIVTRNSAEVKLTATE
                     FK"
BASE COUNT          687 a          799 c          908 g          720 t
ORIGIN      
        1 ggtggagaac ggctgccagc cgtagtcgtt caaatggttg ccggtcaatt caatgatttt
       61 cacgttcacg tctgtcagca agtccatata ttttggatcg ctgcagaaga tgccgcggtt
      121 caatgtcggc tgcttcgggc aatccggatc gaatgacact tcgttgctga tatgcacaat
      181 atcggaagag gtaaagtagt ctttgattaa tgtgtcagga tacagcacac cgttcgtatc
      241 catcgatgcg gcgaagtcgc gcgacatggc cgtcacgccg gtcatcgcga cgatcgtcaa
      301 ctttgattca tcccgattag tgatcgattg accgaaagca gcttgggcgg cttgcgcatc
      361 cccgatcgat cccgccagcc cgacgttcac gatcagcgga tacgtgttga gatcgaggtt
      421 gcgatgcagt ggcgttaagc cgtcaatcga caaaactttc atgcggacat ttaattcatc
      481 gaaaggcaca atcgctaatg cgggttgaat agcccataat ttttgtgtca gctgatcggt
      541 tggcaccact tcgatcgcgt taggcgcggg atcacccaag atcgatctca gcgcttgcaa
      601 agttgaatca gtcagtttga gtgtgccggt gaaggtgcag ccctcgcagg taacgggctg
      661 gccttgccaa gcggattgaa ctgcgctcca cggcacatca tcgatcgcag tggggaacgg
      721 cgcgacgaac gcgaacaccc attggccgat caccggcaca tcggcgtgtg cggtataacc
      781 cacttgcaag ctggccgtgg ccggatcacc cgctaaagta aattgtgccg gttgcgctgt
      841 gatcatttca tcgaggtgcg atcgcacggc ttcgggcagc gagccttgtg agtagattgc
      901 aatcggcttg ggcgcaggtg tcggggcaag tgtcttcgtc ggcacaacgc tgggcgcgat
      961 cgtgtgggtt ggagtgacga tcgggctggc gcaggccgac aggatcagca tcaacatgat
     1021 cgagctgatc accactgaaa atttatacaa tcgagtattc atatcgttta gttttccttg
     1081 tggtctgtgc ttgacaacga ggcggaatga taacactgcg ggcgtgaagc gacaagcagc
     1141 ctggagttgt ataatgacgg ttgaggttca agccatgcgc cgagtatcca cttcgattat
     1201 cgtgccttat ctcatctcca tcgcgcttat ctttttgatc accggcgcat tctatttgct
     1261 gaacatcgaa aacgatacgc cgattgtggc gctgttgtat ctgctgcccg tcggcttgag
     1321 cacggcgctg tggggcttag gtcccggcat cgcttgcgcg gtttcggctt tcttcgtgtt
     1381 caactactat tttattcaac caacttacac tttgaccgtg catcagccgc aggacgtgct
     1441 ggctttgatc gtgttcttag tggtcgcgat tgtcatcagc cagttggtag gccgcgccaa
     1501 gctcaacctg gcgactgcga acacgcggga acacgaagcc agccagttgt acgatttagg
     1561 cacactgttg tccgcttcgc atgacgatgg atcgattgcg cgcgggctgg cctataaggt
     1621 ctcgcaaact tttcaggccg atagggtggg cgttagtctt gaaacccgat tggcttcgca
     1681 agccttattg atcgttgagc cgcatacgtc aacgagcccg atcggcaaac cgacgattat
     1741 tgtgccgttg atgacagcac gtggactgca aggtgagatt cgggtctggc gcaaagaacc
     1801 gctatcgatg ggcgaagaaa gattattgca gactttcgcg acccagggtg gactgtcgct
     1861 cgatcgatcg cgcctggcgc aagcggagac gcgcgcaaaa gtcttggaag agagtgatcg
     1921 cttgaagtcg gcgctgttgt cctcagtatc gcacgaactg cgcacgccgc tggcgacgat
     1981 caaagcttcg atctccagcc tgcgatccga aacggttgat tgggattcgg cggcgcggca
     2041 tgatctcttg gcggcgattg aagaggaaac cgattacttg aatcagctcg tcggcaactt
     2101 gctggacatg tcgcgcatcg aagcaggcgc tttgaatccg cagaagaaat ggaattcgct
     2161 ggccgaaatc gtcggcagtg tgttgagccg gatgcgatcg gctgcgcaag agcaccgcct
     2221 tgaaatcgat ctatcggacg atctcccttt ggtgttagta gattacgtgc agctcgctca
     2281 agtcttcagc aacttgatca gtaacagtct caagtacgcg ccgatccagt ccacgatcaa
     2341 aatctcagcg cgagtcaaac ccgataggtc gatccagatt gaggtgatca atcaaggccc
     2401 gcccgtccca gcggaacatc tcgatcggat cttcgacaag ttctatcggg tcactgcagc
     2461 cgatcgggtg accggcacgg gtttgggctt atcgatctgc aagggtatga tcgaggcgca
     2521 cggcggccgc atctgggcgg agaatcgacc tgaaggtttt gcgtttgtgt tcatgttgcc
     2581 ggccgaatcc gtcaatcaac cgaagatgcc tatcgagaaa gaagcgtcat gacgaatccg
     2641 ccgcgtatat tggtgattga cgacgagccg cagatactgc gcgccatgcg cacgatcttg
     2701 acggcgaaac aatttgaggt catcacggct tcgcgtggtg aagagggtct agcgttggcc
     2761 gccgcgaatc cgccggatgt gattgtgctc gatctcagtc tgccggacat ggacggcatt
     2821 caagtctgcg cgcgcctgcg tgaatggacg cagattccga tcatcgtgtt gtcggtgcgg
     2881 gacaacgagc gcgataaagt tgccgcgctc gataaaggtg cggatgatta tctgaccaag
     2941 ccgttcggca tcgaagaatt actggcacgg attcgcgtgg cgctgcggca ttccgtgcat
     3001 tcgcaaggca gcaagaaaac gatcgtcaat gccggaaccc tgtcgatcga tttggcgaat
     3061 cacatcgtta cgcgcaacag cgcagaagtg aaattgaccg cgacggaatt caag
//
LOCUS       DASRTJ010000691         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_296095,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000691 DASRTJ010000000
VERSION     DASRTJ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296095"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="VFF70_13970"
     CDS             complement(<1..2115)
                     /locus_tag="VFF70_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY45842.1"
                     /translation="MIKLTINDQLIEAREGQTVLEAAQANQITIPTLCYHKDLSPAGS
                     CRLCVVEVDKIRGQVAACTQIVNEGMVAHTETPAIVQSRKMVLQLLLANYHDAGYATA
                     QDPALNELLYWVKRYAVQPPRGLVSQPHYTVNSDPNPFVWVDWNKCILCTRCIRACAE
                     VQGRFVWGVAQRGFDTKIVAGADTNMIEARCESCGACVVYCPTGALDHKLSMNLGQPD
                     RVITTTCTYCGVGCQIDLNIKDNRIIRVTSNATAAVNGLAMCVKGRYGYDFVHHPDRL
                     TKPRVRKYLLEGSGIRAQGSGHPQSAIRNPPFDRGEWIDVDWNTALNIVAKKLIEIKQ
                     QSGPDSIGVLASAKCTNEENYLMQKFARQVIGTHNVDHCARLCHSSTVAGLAMCYGSG
                     AMSNSMEDIADNARAIFIIGSNTTEQHPVFGAKIRQAVLQRGVKLIVADPRKIDITEF
                     ATLHIRQKPGTDVALINGLMHIIFKNNWHDQKYIDERCENFEEFRSTIEKYPPELVSE
                     ITGVSIEQLNQAAELISINKPMAVFWAMGITQHTTGVFNVLSLGNLQMLLGNMGVPGG
                     GVNPLRGQNNVQGACDMGALPNVFPGYQGVTEEKAREKFNRAWQIEDHDWPMDQSSIN
                     PPRPLFTLKDRPGLTVTEMIPQAGEGKVRALYILGEDPMLTDPDLNHVRECLEACEFT
                     VLQEIFPSVTSEYADVLLPGASF"
     gene            complement(2190..>3461)
                     /gene="nuoF"
                     /locus_tag="VFF70_13975"
     CDS             complement(2190..>3461)
                     /gene="nuoF"
                     /locus_tag="VFF70_13975"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZY45843.1"
                     /translation="RSGWRGEPIDYTQPRPGEIRSMLAQAGQIDPDSIDSAIDHGAYR
                     ALDRALQQSPSAIIDEVETSGLQGRGGAGFPVGRKWRFVAQADRSPKYVVCNADESEP
                     LIFKDRVLMDTNPHQLLEGMALAGYAVGAHEAFIYIRGEYASQTDRLERAIQQAEEKN
                     WLGEKIRGTEFSFKVHVHRGAGAYICGEETALLESLEGKRGEPRIRPPFPATFGYHGL
                     PTVVNNVETFSAVPAIMLNGAPWYRSISAASTPGTKLYMLLGHINRPGLFEAPFGLTL
                     RKIIDDFGGGMRSGSKFHFALTGGAAGTLIPERLLDIPIDYSSAAKGVSLGAGAFLIC
                     DQTIAPIDLLRELLHFFEVESCGKCTPCRDGTPAAREILDRMLRGDRQVDDVQRLTEL
                     AEVLQTASFCGLGQSVAIPMKSALTHFSQEF"
BASE COUNT          732 a         1006 c          946 g          777 t
ORIGIN      
        1 aaacgatgcg ccgggcagca gcacatcggc atattctgaa gtcactgacg gaaaaatttc
       61 ctgcagcacg gtaaactcgc acgcctccaa acattcgcgc acgtgattga gatcgggatc
      121 ggtcaacatg ggatcttcgc cgagaatata cagcgcgcga actttgcctt cacccgcctg
      181 cggaatcatc tccgtcaccg tcaggccggg tcggtccttg agcgtgaaca acggtcgagg
      241 cggattgatc gaactttgat ccatgggcca atcgtgatct tcgatctgcc aggcacgatt
      301 aaatttttcg cgcgccttct cttccgtcac gccttgataa ccggggaata cattcggcag
      361 cgcgcccata tcgcatgcgc cctgcacatt gttctggccg cgcagcggat tcacgccgcc
      421 gccgggcacg cccatgttgc ccaacagcat ttgcaaatta cccaggctca acacattgaa
      481 cacgccggtc gtgtgctgcg tgatgcccat cgcccagaag accgccatcg gcttgttgat
      541 cgagatgagt tctgcggctt gattcagttg ctcgatcgat acgccggtaa tttcggatac
      601 gagttcgggc ggatatttct cgatcgtgga tcggaactcc tcaaagtttt cgcagcgctc
      661 atcgatatat ttttgatcgt gccagttatt cttgaagata atgtgcatta agccgttgat
      721 cagcgccaca tcggtgccgg gcttttggcg gatgtgcagc gtggcaaatt cagtgatgtc
      781 gatcttgcgc ggatcggcga cgatcaattt cacgccgcgc tgcaacacgg cctgtcgaat
      841 cttcgcgccg aagaccggat gctgctcggt ggtgttcgaa ccgatgatga agatcgcccg
      901 ggcgttatcg gcaatatcct ccatgctatt gctcatcgcg ccggagccgt aacacatcgc
      961 caaacccgcg acggtcgatg agtgacataa gcgcgcgcag tgatcgacgt tgtgtgtacc
     1021 gatgacctgc cgcgcgaatt tttgcatcag atagttttct tcgttcgtgc atttggccga
     1081 cgccagcacg ccgatcgaat ccgggcccga ttgctgctta atctcgatca gtttcttagc
     1141 gacaatattc agcgccgtat tccaatcgac atcgatccat tcgccccgat cgaatggcgg
     1201 attgcggatt gcggattgcg gatgtcctga tccctgagcc ctaatccccg acccctctaa
     1261 cagatatttt cgtacgcgcg gtttggtcaa ccgatcagga tgatgcacaa agtcatagcc
     1321 gtagcggcct ttcacacaca tcgccaaacc attcacggct gctgtagcat tcgaagtgac
     1381 gcgaatgatc cggttgtctt taatgttcag atcgatctgg cagccgactc cgcagtacgt
     1441 gcaagtcgtg gtgatcactc gatcgggttg gccgagattc atggagagtt tgtgatcgag
     1501 cgcgcccgtc gggcagtaaa cgacgcatgc gccgcatgat tcacagcgtg cctcgatcat
     1561 attcgtatct gcacccgcga cgatcttggt gtcaaagccg cgctgcgcga caccccatac
     1621 gaatcgcccc tgcacttcgg cgcaggcgcg gatacagcgt gtgcacaaga tacatttatt
     1681 ccaatcgacc catacaaacg gattggggtc actgttgact gtgtaatgcg gttgcgacac
     1741 caagccgcgc ggcggctgaa cagcatagcg cttcacccaa tacagcaact cgttcagcgc
     1801 cgggtcttgg gcggtagcat agcccgcgtc atggtaattg gccaacaaca gttgcaggac
     1861 catcttgcgc gactgaacga tcgcgggtgt ttcagtgtgg gccaccatgc cctcattaac
     1921 aatttgagtg caggccgcga cctggccgcg aatcttgtcg acttcgacga cgcacaggcg
     1981 acacgatccc gccggcgaca aatctttgtg gtagcacaac gtcggaatgg tgatttgatt
     2041 cgcctgcgcc gcttcgagca cggtttgtcc ctcgcgcgct tcgatgagtt gatcgttgat
     2101 ggtgagtttg atcatcatta actccaaatt ttatgaaact cggttgaaca cggactgctc
     2161 agatttttat ccttggaacc cgtgtccttc tagaactctt gagaaaaatg tgtcagcgcc
     2221 gatttcatgg gtatcgctac cgattggcct aggccgcaga atgaagcggt ttgcagcacc
     2281 tcggccagtt cagtcaagcg ttgaacatcg tcgacctgtc gatcgccgcg cagcatccga
     2341 tcgagaatct cgcgcgctgc aggcgtgcca tcccggcacg gcgtacactt gccgcacgat
     2401 tctacttcga agaagtgcag cagttcgcgc aagagatcga tcggggcgat cgtttgatcg
     2461 caaatcaaga acgcgcccgc gcccagcgac acgcctttgg cggcagacga gtaatcgatg
     2521 ggaatatcca gtaatctttc cggaatgagc gtgccagctg cgccgccggt cagcgcaaaa
     2581 tggaacttcg atccggatcg catcccgccg ccgaagtcgt cgatgatttt gcgcaatgtc
     2641 aaaccaaatg gcgcttcaaa caaaccgggc cggttgatgt gaccgagcaa catgtacaac
     2701 ttggtgccgg gtgttgaggc cgcagagatc gatcgatacc acggcgcgcc attgagcatg
     2761 atcgccggca cggcggaaaa agtctccacg ttgttgacga cggtcggcag gccgtgatag
     2821 ccaaacgtcg ccggaaacgg cgggcgaatg cgcggctcgc cgcgtttacc ttccagcgat
     2881 tccagcagcg ccgtttcttc gccgcagatg tacgcgcccg cgccgcgatg cacatgcacc
     2941 ttgaatgaaa attcggtgcc gcgaattttt tcgcctaacc aattcttctc ttcggcttgc
     3001 tgaatcgcgc gctccaatcg atccgtttgc gaggcatatt cgccgcgaat ataaatgaag
     3061 gcttcgtgcg cgccgacggc ataaccggcc agcgccatgc cctccagcag ttgatgtggg
     3121 ttcgtatcca tcaacacgcg gtctttgaaa atcagcggct cgctttcgtc tgcattgcag
     3181 accacatatt tcggcgatcg atcggcctgg gcgacgaagc gccatttgcg tcccactgga
     3241 aaacccgcgc cgccgcggcc ttgcaagccc gacgtttcaa cttcatcgat gatggctgac
     3301 ggtgattgct gcagcgcgcg atcgagcgca cgatatgcgc cgtgatcgat cgccgaatcg
     3361 atcgaatcgg gatcgatctg gccggcctga gccagcatgc tgcgaatttc gccgggacgc
     3421 ggctgcgtat aatcgatcgg ttcgccgcgc caacccgatc g
//
LOCUS       DASRTJ010000692         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_370775,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000692 DASRTJ010000000
VERSION     DASRTJ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370775"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFF70_13980"
     CDS             complement(<1..252)
                     /locus_tag="VFF70_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45844.1"
                     /translation="MKSRPRLTRFAVWLPAIVFLLLLLAWEIAARNGRLSALLFPAPT
                     VIGARLVRLLGNGELLNDLGVTGLRMGIGFAVGCGLGTTL"
     gene            complement(249..1025)
                     /locus_tag="VFF70_13985"
     CDS             complement(249..1025)
                     /locus_tag="VFF70_13985"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45845.1"
                     /translation="MRLRCKHISKSYTTRGSITEALVDVSFEVDDHEFVCVIGPSGCG
                     KTTLLKILAGLLEPTSGQVVFDQGLQAAPARRAMVFQEHGLFPWMTLIDNVAFGLKMQ
                     GLDERTRIDRAASWIDRFGLGPFAAHYPRELSVGMRQRAGIARAFLVDPEVLLMDEPF
                     GSLDAQTRLILQDELLKVWLATRNSVIYVTHDIEEAIRLGDRVLVMSGRPGRIRSDIP
                     ILIDRSRRLTDALNGEGSEIKWQIWKTLEDEVRAGLLHSH"
     gene            complement(1037..1312)
                     /locus_tag="VFF70_13990"
     CDS             complement(1037..1312)
                     /locus_tag="VFF70_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45846.1"
                     /translation="MAYLKGVRQYNQGKTDRNLDIVAKYTQQDREVVKDACWQAINAD
                     GQIDLNSIVDFGNWAVKNNLLDVAVPPAQFWDPSFIDYANGILQATP"
     gene            complement(1300..2124)
                     /locus_tag="VFF70_13995"
     CDS             complement(1300..2124)
                     /locus_tag="VFF70_13995"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY45847.1"
                     /translation="MKNRFQNRSILGGLLSVSLLLAACGTVTPSSTAVRPAPTASAAA
                     PTATSPAPTLVPVKIKAAVPLNLSNAPLFIAQAEGYFAEQGLEVEFITVGSSARALPS
                     LISGELDVLAGAISVGHLNAIAHETHIRFVADKGHYETGECEAVSYAVRPDFLAQHPI
                     NGPADLKGLKINVNMATSAGYTLEKFLAQANLTIADVQTFLLDPSAELPALESGQVDM
                     ISVIEPSLSQEVKAGHVAALSGFYRSATTKQGATVIFGASLVDKNPEAGKRFMWRI"
BASE COUNT          558 a          687 c          587 g          519 t
ORIGIN      
        1 aagtgtggtg ccaagtccgc agcccaccgc gaagccgatg cccatgcgca ggccggtcac
       61 gcccagatca ttcaacaact cgccattccc gagcagtcgc accaagcgcg caccgatcac
      121 tgtcggcgct ggaaacaata aggccgagag ccgaccgttg cgcgcggcta tttcccaagc
      181 cagcagcaag agcagaaata cgatcgccgg cagccagacc gcaaagcgag tgaggcgcgg
      241 ccttgatttc aatgagagtg caatagtccg gcgcggactt cgtcttctaa cgtcttccaa
      301 atttgccatt taatttctga accctctcca ttcagcgcat ccgtcaagcg gcgcgatcga
      361 tcgatcagga tcggaatgtc cgatcggatg cggcccggcc gtcctgacat caccaacact
      421 cgatcgccca agcggatcgc ttcttcaata tcgtgcgtga catagatcac cgaattgcgc
      481 gttgccagcc acactttcaa caattcgtct tgcagaatca agcgcgtctg cgcatccagc
      541 gagccaaacg gctcgtccat caacagcact tcaggatcga ctaaaaacgc gcgcgcgatc
      601 ccggcgcgct ggcgcatgcc cacggataat tcacgcggat agtgagccgc gaacgggccg
      661 aggccaaacc gatcgatcca gctggccgcc cgatcgatcc gcgtgcgctc atcgagacct
      721 tgcatcttta agccaaaggc cacgttatcg atcagcgtca tccacgggaa caaaccgtgt
      781 tcttgaaaga ccattgcgcg gcgcgccggt gccgcttgca agccttgatc gaatacaact
      841 tgaccggagg tcggctccaa taaaccggcc agaattttca acaatgtcgt tttgccgcaa
      901 ccgctgggac caatgacaca cacgaattca tgatcatcca cttcaaacga gacatcgacc
      961 agcgcctcgg tgatcgatcc gcgggtcgtg taagattttg aaatgtgctt gcatctcaat
     1021 cgcatgctat gctcgttcag ggtgtcgctt gcagaatgcc attcgcgtaa tcaatgaaac
     1081 tcggatccca aaattgagcc gggggaacgg ccacatctaa caggttgttc ttcacagccc
     1141 agtttccaaa atcaacaatg ctattaaggt caatctggcc gtcagcgttg atcgcttgcc
     1201 aacaagcatc ctttacaacc tcgcgatctt gctgtgtata cttcgccaca atgtccagat
     1261 ttcgatcggt cttgccttga ttatactgac gcacaccttt cagatacgcc acatgaagcg
     1321 tttgcccgcc tccgggtttt tgtcaactag gctcgcgcca aaaataacgg tcgcaccttg
     1381 ttttgtcgtt gccgatcgat agaagccgga caacgctgcg acatggccgg ccttgacctc
     1441 ttgggagagg ctcggttcga tcacgctgat catatccact tggccggact ccagcgcggg
     1501 cagttcagcg gatggatcaa gtaaaaaggt ctgcacgtca gcgatggtga gattcgcttg
     1561 agcgaggaat ttttctaaag tgtaaccggc actcgttgcc atgttcacgt taattttcaa
     1621 gcccttgaga tcggccggtc cgttaatcgg atgctgcgcc agaaagtctg gtcgaactgc
     1681 gtaactcact gcttcacact cccccgtttc gtaatgccct ttatccgcca cgaatcgaat
     1741 gtgagtttca tgggcgatag catttaagtg tccaaccgaa attgcacccg ccagcacatc
     1801 caactcccca gaaatcagcg aggggagagc tcgcgccgaa ctgccgaccg ttataaactc
     1861 cacctccaag ccttgttcag caaagtaacc ttcagcctgc gcgataaaga gcggcgcatt
     1921 agaaagattc agcggaacag cggccttgat ttttactggt actaaggttg gcgcgggtga
     1981 tgtggctgtc ggtgcagccg cgctcgccgt cggcgcaggc cgtacggctg tcgatgacgg
     2041 tgtcacagtg ccgcacgctg ccaacaggag actgacactt agcaatcccc ccaagatcga
     2101 tcgattctga aacctatttt tcattgctac ctcgtattat cgtgaatgag ttaattacat
     2161 gcatgcagtc tgtccggcca aacggccata ttgaaactag gtttgattga atatcggctt
     2221 tgctttttac tagtcggcga ggttgaatat aatcggagca tgaaacgatg cgagcaacca
     2281 cccaccccgc cggccatcgc tgaacaatcg cgtgactgca cgccccgttc gatgtaattg
     2341 cggatcgaga t
//
LOCUS       DASRTJ010000693         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_371267,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000693 DASRTJ010000000
VERSION     DASRTJ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371267"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..495
                     /locus_tag="VFF70_14000"
     CDS             133..495
                     /locus_tag="VFF70_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45848.1"
                     /translation="MYEHHTEPLIPRSEFAKRLLKSFLVSLAIIFGSLIIGMIGYRVT
                     EGLSWIDAFLNASMLMGGMGPVDVMHTDAGKLFAGFYALYSGLVLLIAVGVLGAPLYH
                     RFQHRFHLETDEDQDKDK"
     gene            593..1849
                     /gene="thrC"
                     /locus_tag="VFF70_14005"
     CDS             593..1849
                     /gene="thrC"
                     /locus_tag="VFF70_14005"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZY45849.1"
                     /translation="MKNLIGFKCSICGEEYALDQVEYVCPKHGDEGVLDAIYDYTAIK
                     RQIDPKKLADRQSAASLNLFRYKSLLPIDSESAELPLHIGWTPLYRAPRLEKMLGLKE
                     VWIKDDGRNPTASFKDRASSVVVVKARELKRELITTASSGNAGAALAGMAASVKMPTV
                     IFVPHTAPQAKVAQLLIYGSIVFLVKDNYDAAFELCLQASKEFGWYCRNTAYNPITVE
                     GKKTASLEICEQLNWHAPDRIFVSVGDGNIISGLHAGLRDLAALNWIDHMPKLMGIQA
                     EGSAAVYNAWKANRSEIEPVAGKTIADSINVDLPRDGRRAVRSIRETQGESLTVSDDE
                     ILDAMRVLARETAVFAEPAASTAFAGLLKAAQANRIGKDERVVVVITGSGLKDVSSAI
                     KAAGQAHTIDPDMKAVKAVAQSIGLS"
BASE COUNT          451 a          471 c          569 g          437 t
ORIGIN      
        1 gtggaagtgg gccggaatgg cgctgggtcc gatcgggatg gtcttaggct ggacaccgtt
       61 catccagaaa cgcattcgac ggagcgccaa cgatcgattg gataaattct ttacccatca
      121 aaggtgatcg ccatgtacga acatcacact gaaccgctga tcccccgatc tgaatttgcc
      181 aagcgactgc tgaaatcgtt tctggtgtcg ttagctatca tcttcggctc gctgatcatc
      241 ggcatgatcg gctatcgcgt cacagagggc ttgagttgga tcgatgcgtt tctcaacgca
      301 tcgatgttga tgggcggcat gggacccgtc gatgtcatgc acacggatgc aggtaagttg
      361 tttgcgggct tttatgctct ttattcgggc ttggtgctgt tgatcgccgt cggcgtattg
      421 ggcgcgccgc tctatcatcg cttccagcat cgatttcatt tagagacgga tgaagatcag
      481 gacaaagata agtagggtgg ccgtcattct ttgaattttt gcgcgatgac gcacggctgc
      541 acggcggtct cacggtatga ctgcggggag attagcaaac ctctggagaa gcatgaaaaa
      601 tctaatcggt ttcaaatgca gtatctgtgg tgaagagtat gcgctcgatc aagtggagta
      661 tgtctgtcca aaacacggcg acgaaggtgt gctcgacgcg atctacgact acacggcaat
      721 caagcggcag atcgatccaa agaagttggc ggatcggcag tcggcggctt ccttgaatct
      781 gtttcgttat aaatccctgc tgccgattga ttcagaatcc gctgagctgc cactgcatat
      841 cggttggaca cccctctatc gggcgccgcg actcgaaaaa atgttaggtc tcaaagaagt
      901 gtggatcaaa gacgatggcc gtaatccaac ggcatcgttc aaagatcggg cgagttcggt
      961 cgtcgtcgtc aaggcgcgtg aattgaagcg tgagctgatt acgactgcat caagtggcaa
     1021 cgcaggcgcg gcgctcgccg gtatggcggc cagcgtcaaa atgccaacgg tgatctttgt
     1081 gccgcacact gcgccgcaag cgaaagttgc ccagctgctt atttatggat cgatcgtgtt
     1141 cttggtgaaa gacaattacg atgcggcatt cgagctgtgc ttgcaagcct cgaaagagtt
     1201 cggctggtat tgccgcaaca cggcttacaa tccgatcaca gtcgagggca agaaaacggc
     1261 ctcgctggaa atttgcgaac aactgaattg gcacgcgccg gatcgaatct tcgtcagcgt
     1321 gggcgatggc aacatcattt caggcttgca tgccggcctg cgcgatctgg ccgcgctcaa
     1381 ctggatcgat cacatgccca agttgatggg catacaagcc gaaggttcag ccgccgttta
     1441 taacgcgtgg aaagcaaatc gatctgaaat tgaacccgtc gcgggcaaga cgattgccga
     1501 cagcatcaat gtcgatctac cgcgcgatgg acgccgagcc gtgcgatcga tccgcgagac
     1561 acagggtgaa tcgttaaccg ttagtgatga tgagatactg gatgcgatgc gcgtgctggc
     1621 ccgtgagaca gcggtctttg ccgaacctgc ggccagcact gcgttcgcgg gacttttgaa
     1681 agcagctcag gccaatcgga ttggcaaaga tgaacgcgtg gtggttgtga tcacgggcag
     1741 cggcttgaaa gatgtatcga gcgcgatcaa agcggccggg caagcccaca cgatcgatcc
     1801 ggatatgaag gccgtcaaag ccgtcgccca atcgatcggg ttgagctaaa cctgaggatt
     1861 ataagccgat ctcgcgtgtc ttttcgaatt cgacggggtt agtgtagaat agattaggag
     1921 gaagactg
//
LOCUS       DASRTJ010000694         7679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_371269,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000694 DASRTJ010000000
VERSION     DASRTJ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371269"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFF70_14010"
     CDS             complement(<1..525)
                     /locus_tag="VFF70_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HZY45850.1"
                     /translation="MSDEAIVIQGAVCQPPFQTRDRIMLGHGSGGKLSHDLIRRSFLP
                     AFDNDALRAGDDAGVFDLNSCTRLAFSTDSHVVWPLFFPGGDIGRLAVCGTVNDIAMM
                     GAIPKYLTAGFILEEGLEVDMLQRVIESMRVAAEEANIQIVAGDTKVVQKGKADGLYI
                     NTAGIGVVPIGIEIG"
     gene            complement(522..1613)
                     /gene="hypD"
                     /locus_tag="VFF70_14015"
     CDS             complement(522..1613)
                     /gene="hypD"
                     /locus_tag="VFF70_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HZY45851.1"
                     /translation="MKYLDEYRDAELVKKGLAEIRRIVTRPWSLMEICGGQTHSIMRH
                     GLDELLPPEIDLIHGPGCPVCVTPLELIDKAIAIASQPDVIFTSFGDMLRVPGSDEDL
                     FRTRADGGDVRVVYSPLDAVKIAQQNPDKQVVFFGIGFETTAPANAMSVWQAKHLGVK
                     NYSMLASHVRVPPAMRAILASPYNQVQAFLAAGHVCAVMGYWEYPSIAEEFKIPIVIT
                     GFEPVDLVEGILMAVRQLEANRHEVENAYSRIVTYAGNRPAQEIINTVFVECDRKWRG
                     IGTIPMSGWRLRDEFSEFDAEKHFDVGAISTKESVLCIGGMILQGRQKPIECAAFGKE
                     CTPEHPLGATMVSSEGACAAYYRYRRIEA"
     gene            complement(1664..2308)
                     /locus_tag="VFF70_14020"
     CDS             complement(1664..2308)
                     /locus_tag="VFF70_14020"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZY45852.1"
                     /translation="MATTTHYQIDYSDVPIRLFKSDFLEFFTHITPQAIVVIWTPVIA
                     LFLIRAVQESALAISAWYLLPAFAIGLFIWTFGEYTMHRFVFHYPARTPTMERITFLF
                     HGVHHAQPSLKTRLVMPPIVSIPLALIFYGLFSLAFSALNAPLWIAPMFAGTVTGYLA
                     YDLTHYATHHWPLKWRYLKYLKRHHMLHHFKTENMRFGVTSPAWDYVFHTMPAA"
     gene            complement(2421..2675)
                     /locus_tag="VFF70_14025"
     CDS             complement(2421..2675)
                     /locus_tag="VFF70_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZY45853.1"
                     /translation="MCLGIPGKIIDIYEANGLRMGKVDFGGVTKEVCLSYVPEAKIGN
                     YTVIHVGFALNLLDENEALETLDLLRQIGAVDEELGKEPA"
     gene            complement(2704..4995)
                     /gene="hypF"
                     /locus_tag="VFF70_14030"
     CDS             complement(2704..4995)
                     /gene="hypF"
                     /locus_tag="VFF70_14030"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HZY45854.1"
                     /translation="MSPTSIGKHIHISGIVQGVGFRPFVYGLALRHELHGEVRNTSSG
                     VDISIEGAADQIDHFIRSLTIEAPPLAKIDSIEVQAAQANGFTVFSIVDSVSQVGAFQ
                     PISPDIGLCDDCRHELIDPTNRRYRYPFINCTNCGPRFTIIQDIPYDRPLTTMAPFVM
                     CPDCAKEYVDPLDRRFHAQPIACPKCGPNVWLQMNADEQPDANEAIQKVQHLLADGKI
                     VAIKGLGGFHLACDATHDAAVAELRRRKGRIDKPFALMAANLESIEQFCEVNDQERAL
                     LQSPQRPIVLLNKCSDSKISQHVAPSQSTLGVMLPYTPLHELLFYPSTLSPHPLVMTS
                     GNFSEEPIAIANDDALARLALLADAFLLHNRDIHLRCDDSVVRVFENDELPIRRSRGF
                     APYPVKLLFKAPPILATGGELKNTFCVTRDGYAFLSQHIGDVENYETLKSLEDSVTHF
                     EKLFRIQPQIIAYDLHPDYLASRYALQRAEREIIQSIGVQHHHAHIAACMVENNLADR
                     QAVIGVAFDGTGYGTDGAIWGGEFLVADYHSYIRAAHLKYVPLPGGDTATRHPYRVAL
                     SYLRSAGVNWTDDLAPVQAADPIERTTIAKQIEMKINAPLTSSMGRLFDAVASMLNVR
                     QEVNYEGQAAIELEALADRSETSAYPVSIDAQAEIDTAQIIRSVVADVRSGVPRSIVS
                     ARFHNSIALIVREVCKSIRDRYGVNEIALSGGVFQNVTLLKRTLDLLRAENFKIYTHR
                     LVPPNDGGIALGQAVIAAASLEH"
     gene            complement(5291..5854)
                     /locus_tag="VFF70_14035"
     CDS             complement(5291..5854)
                     /locus_tag="VFF70_14035"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY45855.1"
                     /translation="MNADAIKWNERYQEGVEAAFEQPRSFLIEAAEYLPRYGLALDVA
                     MGLGGNAGFLIERGLRVIGLDISEVAAQRAKQRQPQLMAAVIDLNRFNLATANFDVVI
                     NFYYLQRALWPLYRRVVRSGGVLIFESLLIDTLQVRPDFNPEYLLQRGELRSAFSDWD
                     ILIYREGWLNTDCDSPRAISSLVARQR"
     gene            complement(5857..6885)
                     /locus_tag="VFF70_14040"
     CDS             complement(5857..6885)
                     /locus_tag="VFF70_14040"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZY45856.1"
                     /translation="MSQIKMYQIIKPLLFKLDPERAHSLTLRLIKWAGIFPPAYWLLQ
                     RIFEVNDPRLAVEAFGLKFKNSIGLAAGYDKNGVAVKGLSVLGFGHIEIGTVTLQKQI
                     GNLKPRVHRVPAVRGLINSMGFPNDGVDALRIKRGNAKIGINIGKSKETAIDRAAEDY
                     CELLKRVYTLADYVTVNVSSPNTLNLRQLQAREAISVLLKEICSVRNGLSTRVPLLVK
                     IAPDLNEAEIDDVLDAIALSGIDGIIATNTTIGRAGLSEKYQNLKGGLSGAPLRDRST
                     AIIRYIANRSGGKLPIIGVGGIVEPRDAIEKLAAGAKLVQIYTGLIYSGPSLVKQINQ
                     SLLHARSD"
     gene            complement(6953..>7679)
                     /gene="pyrE"
                     /locus_tag="VFF70_14045"
     CDS             complement(6953..>7679)
                     /gene="pyrE"
                     /locus_tag="VFF70_14045"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZY45857.1"
                     /translation="SRSIAQAADSAAEAKKVRDEINRWRLEIGNSKLVKATPTFNLQH
                     PTSYSQLANDLIDSQCVRFGDFKLKSGVLSPIYLDLRRLVSHPAILQRVAQAYAEKLR
                     ELNFDRLAGIPYAALPIATAIALEMNRPLIYPRREVKDYGTQAVVEGDYLAGETIAVI
                     DDLATTGDSKIEAIEKLESVGLIVKDIIVLIDRGQGAGKMLAEAGYQLHAVVTLPDLL
                     DEWLRSGAISQVQLDQVIVFLTC"
BASE COUNT         1863 a         2117 c         1914 g         1785 t
ORIGIN      
        1 acctatttca attccaatcg gcacaacacc aatcccggcg gtgttgatat acaacccatc
       61 ggccttgccc ttttgtacaa ccttcgtatc gcccgcgaca atttgaatgt ttgcttcttc
      121 tgctgcaacg cgcatcgact cgattacacg ttgaagcata tccacttcaa gtccttcttc
      181 aagaataaaa cctgcagtga gatacttggg tattgcaccc atcatcgcaa tgtcgttgac
      241 cgtgccgcac actgccaggc gaccgatatc gccgccggga aagaacagcg gccacaccac
      301 atgcgaatcg gtgctgaacg ccaaacgcgt gcagctgtta agatcgaata cgcccgcatc
      361 atcacccgcg cgcaatgcat cattgtcaaa tgcaggcaaa aatgatcgcc taatgagatc
      421 gtgcgacaac ttgccgccgc tgccatgtcc caacatgatc cgatcgcgtg tttggaaagg
      481 cggctgacaa actgcgcctt gaatgacgat ggcttcatcg ctcatgcttc gatccttcga
      541 taccgataat acgcggcgca cgcgccttcg ctcgatacca tcgtcgcacc gagtggatgt
      601 tccggcgtgc actctttgcc gaatgcggca cactcgatcg gtttttgtcg gccctgcaag
      661 atcatgccgc cgatgcacaa caccgattct ttcgtgctga tcgctccaac gtcgaagtgc
      721 ttctccgcat cgaactcact gaattcatcg cgtaagcgcc aaccgctcat cgggatcgtg
      781 ccgatgccgc gccactttcg atcgcattcg acaaagactg tgttgatgat ctcttgagca
      841 ggtcggttgc ctgcgtatgt gacgatgcga ctatatgcat tttcaacttc gtgccgatta
      901 gcttccaatt gccgaacggc catcaagata ccttcaacca gatcgaccgg ttcaaaacca
      961 gtgatgacga tcggaatctt gaactcctcg gcgatcgagg gatattccca atagcccatc
     1021 accgcacaca catgaccggc cgcgagaaag gcttgaactt gattgtacgg cgaggctaag
     1081 atcgcgcgca ttgccggcgg cacccgaaca tgtgaggcca gcatcgaata attctttacg
     1141 ccgagatgtt tcgcctgcca gacactcatc gcattcgcag gcgccgtcgt ttcaaagccg
     1201 ataccgaaga acaccacttg cttgtcggga ttctgctgcg cgatcttcac cgcatcgagc
     1261 ggcgagtaaa cgacccgcac atcaccgcca tccgcgcgcg tgcgaaacag gtcttcatcc
     1321 gatccgggca cacgcagcat atcaccgaag gaagtgaaga tcacatcggg ctgtgaagca
     1381 atcgcgatag ccttatcgat caattcgagc ggcgtgacgc acactggaca gcccggcccg
     1441 tggatcaagt cgatctccgg cggcagcagt tcatcgaggc cgtgccgcat gatcgagtga
     1501 gtctgcccgc cgcagatctc catcagcgac cacggccgcg tgacgatccg tcggatttcg
     1561 gccagaccct tttttactaa ttcagcatcg cgatactcat caaggtactt catctattcc
     1621 ccacgaacct tctgagtttc caaaacctga aaggtctaca atcctatgcc gctggcatgg
     1681 tatgaaagac ataatcccac gcgggcgagg tcacaccgaa gcgcatattc tcagtcttga
     1741 aatggtgcag catatggtgg cgcttgagat acttgagata gcgccacttc aacggccaat
     1801 gatgtgtggc gtaatgcgtg agatcgtagg ccaggtaacc cgtgactgtg cctgcaaaca
     1861 tcggcgcgat ccacaatggc gcattgagcg cgctaaacgc cagtgagaac aaaccgtaga
     1921 agatcaacgc caatggaatg ctgacgatgg gcggcatcac gagtcgcgtt ttcaagctcg
     1981 gctgggcgtg atgcacccca tgaaacaaga atgtgatgcg ctccatcgtt ggggtgcgcg
     2041 ccggataatg aaatacaaaa cggtgcatgg tgtactcgcc aaatgtccag atgaacaagc
     2101 cgatcgcgaa ggcaggcagc agataccaag ccgatatggc taaagcactc tcctgcaccg
     2161 cgcggatcaa gaacaacgcg atcaccggcg tccaaatcac gacgatcgct tgtggggtaa
     2221 tgtgggtaaa gaattccaga aagtcggact taaacaaccg aatcggtaca tcgctgtagt
     2281 caatttgata atgggttgtt gtcgccatgt tgcctcctct tcatggaatg actcttatag
     2341 attgggcgcg tcattctggc aaagggatgc cagaacgact gactcgtaat cgtagtgatg
     2401 gcatcctcac accatcgttc tcacgcaggt tctttcccta gttcttcatc gaccgcgccg
     2461 atctgccgca gcagatcgag cgtctctagc gcttcgtttt catccaataa attcaacgca
     2521 aagccgacat gaatcacggt gtaattgccg atcttcgctt caggcacata cgacagacac
     2581 acttcctttg tgacaccgcc gaaatctact ttgcccatac gcagcccatt cgcttcatag
     2641 atatcgatta ttttgccggg gataccaaga cacatagtga ctccaatttg caattagcaa
     2701 taatcaatgt tcaagacttg ccgccgcgat cacggcttga cccaatgcaa tgccgccatc
     2761 gttcggcggg acgagtcgat gcgtgtagat tttgaaattc tccgctcgaa gcaaatcgag
     2821 tgtgcgcttc aagagagtaa cgttctgaaa tacgccgccg ctgagtgcaa tttcgtttac
     2881 gccatatcga tcgcggatcg atttacacac ctcgcgcacg atcagcgcga tcgaattgtg
     2941 gaagcgcgcc gatacgatcg atcttggcac acccgatcgg acgtcagcaa caaccgatcg
     3001 aatgatttgg gcagtatcga tctccgcttg tgcatcgatc gatactgggt aagcgctcgt
     3061 ttcagatcga tcggctaatg cttctaactc gatcgcagct tgaccttcgt aattaacttc
     3121 ttggcggaca ttcagcatcg aggcgactgc atcaaacaac cgccccatgc tggaggttaa
     3181 tggcgcattg atcttcattt caatttgttt ggcaatggtc gttcgctcga tcggatctgc
     3241 ggcttgtacc ggggcaagat catccgtcca attaacacct gcgctgcgca gataactcag
     3301 cgcgacgcga tacggatgcc gcgtggctgt atcgccgccg ggcagcggta catatttcaa
     3361 atgcgcggct cgaatgtagc tgtgataatc agcgaccaaa aactcaccgc cccagatcgc
     3421 gccatcggta ccgtagcctg tgccatcgaa cgccacgccg atcaccgcct gacgatcagc
     3481 caaattgttt tcaaccatgc acgcggcgat gtgcgcgtga tgatgctgca cgccgatcga
     3541 ctgaatgatt tcgcgttcgg ctcgctgcag tgcgtaacgc gacgcgaggt agtccggatg
     3601 aagatcgtat gcgatgattt gcggctggat gcgaaacagt ttctcgaaat gagtcacgct
     3661 atcttcaagc gatttcaacg tctcatagtt ttccacgtcg ccgatatgct gactgagaaa
     3721 tgcatagcca tcgcgggtga cacaaaaagt atttttgagc tcgccacctg ttgctaaaat
     3781 cggcggcgct ttgaacagta atttcaccgg atacggcgca aagccgcgcg atcggcggat
     3841 gggcagttcg tcgttttcaa acactcgcac aacggaatca tcgcagcgca ggtgaatgtc
     3901 gcgattgtgc aagaggaagg catcggctaa cagcgcgagt cgcgcaagcg cgtcatcgtt
     3961 agcgatcgcg attggttctt cgctgaagtt accgctggtc atgactaaag gatgagggct
     4021 gagggttgaa ggataaaaga gtaattcgtg caatggtgta tatggcagca tcacgccaag
     4081 ggtcgactgg ctgggcgcga cgtgttggga aatttttgaa tcactacatt tgttcagcaa
     4141 aacaatcgga cgttgcggcg attgcagcag cgcgcgttct tgatcattga cttcacagaa
     4201 ttgctcgatc gattcaagat ttgcggccat caacgcaaac ggtttatcga tgcggccttt
     4261 gcggcggcgt agttcggcga cagcggcgtc atgcgtcgcg tcgcaggcga gatgaaaacc
     4321 gccgagtcct ttgatcgcaa cgatctttcc gtcagccaaa agatgttgaa ctttttgaat
     4381 ggcctcgttt gcatcaggct gttcatccgc attcatctgt aaccagacat tcggtccgca
     4441 ctttggacac gcgatcggtt gtgcatgaaa tcttcgatcg agcgggtcga catattcttt
     4501 cgcgcagtcc ggacacatta caaacggcgc catcgtcgtc agcggtcgat cgtacgggat
     4561 gtcttgaatg atggtgaaac gcgggccgca gttggtgcag ttgatgaatg gataacgata
     4621 gcgccgattg gtgggatcga tcaattcgtg gcggcaatca tcgcacaagc caatatcggg
     4681 cgaaatcggt tgaaaggctc cgacctgtga gaccgaatca acgatcgaga aaacagtaaa
     4741 accgttcgct tgtgcggcct gcacttcgat cgaatcgatc ttggctaggg gcggtgcttc
     4801 gattgtcaac gatcgaatga aatgatcgat ctgatctgcc gcaccctcga tcgaaatatc
     4861 gacacccgat gaggtgttgc gcacttcacc gtgcagttcg tggcgcaggg cgaggccgta
     4921 tacaaagggt cgaaagccga caccctgtac gatgccgctg atatgaatgt gcttaccaat
     4981 cgatgtcgga ctcatgctga cactcacgct acagtgcaac aaactcagcc acgagacctc
     5041 ctggcgattc ggcggtggtg ggctattaag atagatacgc ttatattatt ttcgcccagg
     5101 ttgcggagat tcaatagtgg acatagtcat ctcgcactgc gccaagtgtc acagcccgtc
     5161 caagcggcag gctcgcacac acccatgcaa cagccgagcg gcgcgtggct ctaaataatc
     5221 acgtcaattc attcaatccg ctcacagaaa gtgggggtgg gcggcgggtc attgaacgta
     5281 cggggaaact ctaccgctgg cgcgcgacca agctcgaaat cgcgcggggt gaatcacaat
     5341 ctgtattgag ccaaccttct cgataaatta agatatccca atccgaaaac gcgctgcgca
     5401 gttcaccacg ttgaagcaga tactcggggt tgaaatccgg ccgcacttgt aatgtgtcga
     5461 tcaacaatga ttcaaagatc aacacgccgc ccgatcgaac cacgcgccga tacaacggcc
     5521 agagagcgcg ctgtaaataa tagaagttga tcacaacgtc gaaatttgcg gttgccaaat
     5581 taaatcgatt gagatcgatc acggcggcca tcaactgcgg ctggcgctgc ttagcgcgct
     5641 gggctgctac ttccgaaata tcgagaccga taacacgcag accacgctcg attaagaaac
     5701 ccgcgttgcc gccgagcccc atcgccacat ccaacgccaa tccgtatcgc ggtaaatatt
     5761 ctgcggcctc gatcaagaac gatcgcggtt gttcaaaagc cgcttcgact ccctcctgat
     5821 aacgctcatt ccatttaatc gcgtcagcgt tcattcttaa tccgatcgag catgtaacag
     5881 cgattgatta atctgcttca ccaaactcgg tcccgaataa attaagccgg tgtaaatttg
     5941 aaccagcttt gctccagcgg ccagtttctc gatcgcatcg cgtggctcga cgattccgcc
     6001 gacgccgatg atgggcagct tgcctcctga tcgattagcg atatagcgaa tgatcgctgt
     6061 tgatcgatcg cgcaatggcg cgccgctcaa gccgcctttc aggttttgat atttctccga
     6121 tagtcccgcg cgcccgatcg tggtgttggt cgcaataatg ccatcgatgc cggagagcgc
     6181 aatcgcatct agcacatcat caatctcggc ttcgttcaag tcaggcgcga tcttcaccaa
     6241 taacggcacc cgcgttgata agccatttcg cacggaacaa atttccttca acaaaaccga
     6301 aatcgcttca cgcgcttgca attggcgcaa gttcagggta ttggggctgc tgacattcac
     6361 tgtcacataa tcggctagtg tgtaaactct cttcaacaac tcacaataat cttctgccgc
     6421 gcgatcgatc gccgtctctt tggatttacc aatattaatc ccaatcttcg cgttcccgcg
     6481 tttgatcctg agcgcatcaa caccatcatt cggaaagccc atgctattga ttaagccgcg
     6541 cactgccggc acgcgatgca cacgcggctt caggttaccg atctgttttt gtaaagtgac
     6601 tgtaccgatt tcgatatgac cgaaacctag cacactcaac cccttgaccg caacaccgtt
     6661 cttatcatag ccggctgcca atccaatcga attcttgaac ttcaggccaa atgcttccac
     6721 cgccaatcgt ggatcgttaa cctcaaatat ccgctggagt aaccagtagg ccggcgggaa
     6781 tattccggcc cacttgatca atcgcaatgt caggctatgt gcgcgctcgg gatcgagctt
     6841 gaagagaaga ggtttgataa tttgatacat ttttatctgt gacatgcaga catgctgacc
     6901 ggatgacaaa ccgacttctc atgacagatc atcatgtcat ctctacacct tgtcagcatg
     6961 tcaggaatac tatcacttga tcaagttgca cttgtgagat cgcgcctgat cgtaaccatt
     7021 catccagcaa atccggcagc gtgactactg catgcaattg atacccggcc tcggctaaca
     7081 ttttgccagc cccctgccct cgatcgatca acacgatgat gtctttgact atcaagccaa
     7141 cgctttctaa tttctcaatc gcttcgatct tcgaatcgcc ggtcgttgcg agatcatcga
     7201 tcacagcgat cgtttcaccc gcaagatagt cgccttcgac gactgcctgt gtgccgtaat
     7261 ctttcacttc acgacgcgga tagatcagcg gtcgattcat ttcgagcgcg atggcggttg
     7321 caattggtaa ggccgcatac ggtatgcccg ccagtcgatc gaagttgagt tcgcgcaact
     7381 tttccgcgta agcttgtgcc acccgctgca agatcgccgg atgtgagacc aagcgccgca
     7441 gatcaagata gatcggcgat aggacgcccg acttgagttt gaaatcgccg aagcgaacgc
     7501 actgcgagtc aataagatcg ttagcaagtt gtgaataaga agttggatgt tggaggttga
     7561 aagttggagt agccttgacc aacttcgaat taccaatttc taatcgccat cggtttattt
     7621 cgtcccggac ctttttcgct tctgctgctg aatcagccgc ttgtgcaatt gaacgagag
//
LOCUS       DASRTJ010000695         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_296879,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000695 DASRTJ010000000
VERSION     DASRTJ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296879"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VFF70_14050"
     CDS             <1..1713
                     /locus_tag="VFF70_14050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45858.1"
                     /translation="DGKLPRSRSPQRLIAAAISVLMFAAVVLQLLALRTERSDPFAVL
                     FDRSVDVAANGYFTVGTRITDVNNFLRNYPALMPTFPIHPQVHPPGVPLLYWATASVL
                     ETSPSLARSIGLWLRSMECNNIDLMLLSDPQLGSALIGMFLPLLANMLTIWCVYKLAR
                     RRFGQRAGLYAAAFWVIVPSAVLFAGNWSQVYPCLACLTWLSVDRGLAQRKVRWFLLA
                     GLVMSIAAFMELGTAALGISLICYILADYLSHRRNLLRDWKFLGAALITSLVGVFSIW
                     IVYQAIEGVSLIQIVQTMLPIHTGYEFDRLTWIFNHPYEFSSFLGIGFAGLLIFISIR
                     SIRQAKNGQPDPLTLSLGLSLIVLTLIDPARDETARTWMIFMPLAIVVISQLMAEPTG
                     DAKRFGVVWTLVALQLLVMIATLQVIQVGLSGPPSTRSTLSSIPSNISSVQVNFGDMV
                     DLIGYRIDRPAPDQLSLDLYWKTVGPIDQPYVVFNHVLNQQGKIIAQQDSRPQNGQPL
                     MTCWQPGEIFLDQHIIPIDPQAASGKVNLELGLYDAVTGIRVSVEFNGITSDHLNLDV
                     AQHN"
BASE COUNT          381 a          437 c          488 g          537 t
ORIGIN      
        1 gatgggaagc tgccccgcag tcgatcgcct caacgcctaa tagcggcagc gatcagtgtc
       61 ttgatgtttg ccgcagtcgt gctgcaactc ctcgcgttgc gcaccgaacg cagcgatcca
      121 tttgccgtac tgtttgatcg atcggtggat gtggccgcta acggttactt tacggttggc
      181 acgcgtatta ccgacgtgaa caacttcctc cgcaattatc cggctctcat gccgaccttc
      241 cccattcatc ctcaagtcca tccgccgggc gtgccactct tgtattgggc gactgcttcc
      301 gttttagaaa catcgccatc gctagctcga tcgatcgggt tgtggttgag atcaatggaa
      361 tgcaataata tcgatttgat gttgttatct gatccacaat tgggcagcgc actgatcgga
      421 atgtttctgc cgctgttggc gaatatgctg acaatctggt gtgtctataa attggcgcgg
      481 cggcgattcg gccagcgcgc cggtttatat gcggcggcct tttgggtgat tgtcccatcg
      541 gcagtattgt ttgccggaaa ttggtcacaa gtctatccgt gtttggcatg tctgacttgg
      601 ctgagtgttg atcgtggcct ggcgcagcgg aaagttcgtt ggttcttgct ggcagggctg
      661 gtcatgtcaa tcgcggcctt tatggaactt ggcacggctg ctttaggcat aagcctaata
      721 tgttatatct tggcggatta tcttagccat cgacgcaact tgttacgcga ttggaaattc
      781 ttgggtgctg ctttgatcac cagcctggtt ggcgtttttt cgatctggat cgtttatcaa
      841 gcgattgagg gtgttagcct gatccaaatc gttcagacga tgctgcccat tcacaccggc
      901 tatgaattcg atcgattgac ctggattttc aatcatccct atgagttttc gagcttcttg
      961 gggatcggct ttgccggcct gttaatcttc atttcaatcc gatcgatccg gcaagcgaaa
     1021 aacggccagc ccgatccgct gacgttatcg cttgggctaa gtttgatagt gctgacactt
     1081 atcgatccgg cgcgcgacga aactgcacgc acttggatga tcttcatgcc gttagcgatt
     1141 gtggtgattt cacaactcat ggctgagcca acgggggacg ctaaaagatt tggcgttgtc
     1201 tggactttag ttgcgcttca attgctggtg atgatcgcga cgctgcaagt gattcaagtg
     1261 gggctatctg ggccgccgtc gactcgatcg actttaagtt ctataccatc gaacatctct
     1321 tcagttcaag ttaattttgg tgatatggtt gacttgatcg gttaccggat tgatcgcccc
     1381 gcgccggatc aattgtcact ggatttatat tggaaaaccg ttggaccgat cgatcagccc
     1441 tacgtggtgt ttaatcatgt gctgaatcag caggggaaga ttattgctca gcaggacagt
     1501 cgtccgcaaa acggacagcc attgatgacg tgctggcagc caggcgaaat atttttggat
     1561 cagcacatta ttccgattga tcctcaagcc gcttcgggca aagtgaattt ggaattgggg
     1621 ttatacgatg cggttaccgg cattcgtgta tcggtcgaat tcaacgggat cacgtctgat
     1681 catcttaatc tggatgtggc acagcacaat taagccttct tgttttagcg ctggttgcga
     1741 ggtcaagtgt aaaatcccta atccgtctgt ggtatacttt tcttcgctgg tcctctcaaa
     1801 agtgtcttcg tattcaaagg gagcaagttt caatgagcag cct
//
LOCUS       DASRTJ010000696         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_143826,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000696 DASRTJ010000000
VERSION     DASRTJ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143826"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFF70_14055"
     CDS             <1..543
                     /locus_tag="VFF70_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZY45859.1"
                     /translation="NGNFGFLFREKAHRPFLNDRAEALGVPFGPERKRLVAGESIQLA
                     DGRMIQPSDVLGAEVPGATLAIVGDASRTDTLIEYVREADALVIESTYLEVEADLARA
                     YGHLTAAQSARLAQAAQVRKLILTHVSRRYSEKEVSAEAQTIFPESVVARDFDHYRIQ
                     KDKSALVIDVPVEEETDVPA"
     gene            530..820
                     /locus_tag="VFF70_14060"
     CDS             530..820
                     /locus_tag="VFF70_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45860.1"
                     /translation="MSLRKASVLEMATAAYELDASVRRGLLRKIDGKWTVMGDDLDNL
                     LNKFDGQEVVLIAASLSDERPLETRTCRTCGREYVGLECPHCRDARIRLRGR"
     gene            922..1608
                     /locus_tag="VFF70_14065"
     CDS             922..1608
                     /locus_tag="VFF70_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZY45861.1"
                     /translation="MSDRLTTLQQLPLFSALSLDQIASFADRFRASTFDQNATIFLEG
                     DPADRLWVIQEGQVKIVKYGEGGKENLLEVIMPGEMFGGAGILFPIHPASAVAMTAVA
                     VLGLPRTEYLQLIRSFPDVALKIIAILGERLRAAMKMRAMSIERVDARLAHILLKLAD
                     KIGVKVDEGVRIDMSLSRQDLADMAGTTIETAIRIMSKFRKDGLVMTEPGGFIVIMEV
                     DRLAALAEGQ"
     gene            complement(1684..2535)
                     /locus_tag="VFF70_14070"
     CDS             complement(1684..2535)
                     /locus_tag="VFF70_14070"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HZY45862.1"
                     /translation="MLTSSITAAVQPGLKAKVNLRFKPPLPKEHGSWAMLAVPLLISL
                     AIAPAWRWSSLLAVVAVIGLFLVRYPLETMIKTKRRDSVLIVWAIIYASLTILCGGWL
                     LVIDRLIWLIPIGLIGAALVLLRWRLALRRQEMSALGELSGIAALALGAPIVYYAVSG
                     QLDWTALALWLINSLYFGGTVFYIKLKVRQQPRVPAPDRISERLIKAKACLTYQAVTL
                     IIVVVLGLLQSVPMLIPLAFVPMTLKVIVGAYHWQDKHSLSLPRLGIIELVHSVLFAA
                     LVMAALK"
     gene            complement(3014..3403)
                     /locus_tag="VFF70_14075"
     CDS             complement(3014..3403)
                     /locus_tag="VFF70_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZY45863.1"
                     /translation="MKVTRIQMSLLLIALAVALAACGGSTVTPKSSMPTGDAKKGQAV
                     FIGTCSACHGPDAKGLPGLGKDMTISTFIKGMTDDEFFKFVHVGRPASDKLNTTGVDM
                     PPKGGNPALTEQDLANVIAYIRTLNKP"
BASE COUNT          804 a          873 c          978 g          783 t
ORIGIN      
        1 aacggcaact ttggtttttt gtttcgcgag aaagcgcatc gacctttcct gaacgatcgg
       61 gctgaggcgt taggcgtgcc tttcgggccg gagcgcaagc gcttggtcgc cggtgaatcg
      121 atccagttag ccgacggccg catgattcaa ccatcagatg tattaggagc cgaagtgccc
      181 ggcgcaacac tcgccatcgt cggcgatgcc agtcgcacgg atactttgat cgagtatgtg
      241 cgcgaggccg atgcgctggt gatcgaatcg acttacttgg aggttgaagc cgatctggcg
      301 cgcgcgtacg ggcatttaac ggcggctcag agtgcgcggt tggcccaagc tgctcaagtg
      361 cggaaattaa tcctgacgca tgtttcgcgg cgttacagcg aaaaagaagt tagcgcggaa
      421 gcccagacga tttttccgga gtcggtggtg gcgcgcgatt ttgatcatta ccggattcag
      481 aaagacaaat ctgctttggt gatcgatgtg ccggttgagg aggagacaga tgtccctgcg
      541 taaagcgagt gtattggaaa tggcgacggc cgcatatgaa ttggacgcca gcgtacggcg
      601 cggtctgctg cgaaaaatcg acggtaagtg gacggtgatg ggcgacgacc tcgataattt
      661 attgaacaaa ttcgacggac aagaagtcgt gctcatcgcc gcttcattga gcgatgaacg
      721 acctctggaa acgcgcacct gccggacatg cgggcgcgag tatgtcggtc tggaatgtcc
      781 acactgccgc gatgcccgca tccgattgcg cggaagatag agcaatgatc aatgtacaat
      841 gtgccatgat caattttcaa aacacaccaa gattgatcgt tgttcattga gcattgttaa
      901 ttgttcgttg ttcagtttcc aatgtccgat cgcttaacca ctcttcaaca gctgcccctg
      961 ttctcggcat tatccctcga tcagatcgcg tcgttcgccg atcggttccg cgccagcacg
     1021 ttcgatcaaa acgcgacgat ctttttggaa ggtgatcccg ccgatcggtt atgggtgatc
     1081 caagagggtc aggtcaagat cgttaaatac ggcgaaggcg gtaaagagaa tcttctcgaa
     1141 gtcattatgc ccggcgagat gtttggcggc gcgggcattt tgtttcccat tcatccggcc
     1201 tctgccgtcg ccatgaccgc tgtggccgtg ttgggtctgc cccgcaccga atacttgcaa
     1261 ctcattcgat cgtttccaga tgtcgcgctg aaaatcatcg ccatcttagg tgagcgcctg
     1321 cgcgcggcga tgaagatgcg cgcgatgtcc atcgaacgcg tcgatgcgcg gctggcgcac
     1381 atcttgttga agttagcgga taagatcggt gtaaaagtcg atgaaggcgt gcggatcgac
     1441 atgtcgttgt cgcgtcaaga cttggccgac atggcgggca caaccatcga gacggcgatc
     1501 cgcatcatga gcaagttccg caaagatgga ttggtgatga ccgagccggg cggctttatt
     1561 gtaattatgg aagtcgatcg cttggccgcg ctggctgaag ggcaataaat tattgcggcg
     1621 agcacttggc acgtggtcct gagaaatatt ctggtcgggg cgatcgattg atcgccccga
     1681 cgtttattta agggcagcca tcaccagcgc cgcaaacaat accgagtgaa ctaactcgat
     1741 tattcctaac cgcggcaggc tgagtgagtg tttgtcctgc cagtgatagg cacccactat
     1801 aactttcaaa gtcattggca caaaggcgag cgggatcaac atcggaacag attgcaacag
     1861 cccaagcacg accacaatta ttaatgtcac agcctgatac gttaagcagg ctttggcttt
     1921 gattaatcgt tcgctgattc gatcgggcgc aggcacgcgc ggctgctgcc gcactttcag
     1981 tttaatataa aaaacggtgc cgccaaaata cagcgagttg atcagccaca aggccagcgc
     2041 cgtccaatcg agttggccgc tgaccgcata ataaacaatc ggcgcgccaa gcgcgagagc
     2101 agcgataccg gacagttcac ccagcgccga catttcttgt cgacgcagcg ctaatcgcca
     2161 acggagcagt accaacgccg cgccgatcag accgatcggg atcagccaga tcaatcgatc
     2221 gatcacgagc aaccagccgc cgcataagat tgtcagacta gcgtagatga tcgcccaaac
     2281 aatcaatacg gagtcgcgtc gctttgtctt gatcatcgtc tccaacggat aacgcaccaa
     2341 gaatagaccg ataaccgcga caacagccaa gagtgatgac caacgccacg ccggggcgat
     2401 tgctaaactg atcaacagtg gcactgccag catcgcccac gagccatgtt ctttgggaag
     2461 cggtggtttg aagcgcaagt tcacttttgc tttaagtccc ggctgaaccg ccgctgtgat
     2521 tgacgacgtc agcacaagtt ttcgcatgga ggggacgcgg cgaaacgcgg gcggcttggg
     2581 cgcgcagttc aacattgcaa gtggactggc atttgacggg taacgggtga tcaaatgtgt
     2641 gaccgcagtt gtggcacttg gcttcaacgg cgtaacatca tacttccact tccggttcgt
     2701 tgcagtcagg acacgtcaca tagtccggaa agggtcgagc taaatcgatc tcagtcatag
     2761 tccatctcgt tccgttttcg attgaatgcc agtatagccg atggatcgat ctgcgcatat
     2821 gatgcacgtc ataaattgca gcggagaggt gtcatttatt ggacgggatt agcgctaaca
     2881 aaacgcgacg cccctgatcg gggggagatc aagggcgtcg ctagccaagg gagggagact
     2941 acctaaattg atgtaacaca ctgagtctag atcagccagc tgctgatgta tatgatctag
     3001 atcataattc cgattagggt ttgttgagcg tgcggatgta ggcaatgaca ttcgccaagt
     3061 cttgttcggt cagcgccggg ttgccgcctt tgggtggcat gtcgacgccg gtggtattta
     3121 acttatcgct ggccggtcga ccgacgtgca cgaatttgaa gaactcatcg tcagtcatgc
     3181 ctttgatgaa tgtgctaatg gtcatgtctt tgccgaggcc gggcagacct ttcgcatccg
     3241 ggccatgaca ggccgagcag gtgccgatga agacggcttg acctttcttt gcatcgccgg
     3301 tcggcatcga tgattttggc gtgaccgtgc tgccgccgca ggcggctaaa gcgactgcta
     3361 acgcgatcag cagcaggctc atttgaatcc gagtgacttt catgtcattt cctccaaaaa
     3421 aatttgatag tgatattg
//
LOCUS       DASRTJ010000697         6398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_297161,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000697 DASRTJ010000000
VERSION     DASRTJ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297161"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..647
                     /locus_tag="VFF70_14080"
     CDS             207..647
                     /locus_tag="VFF70_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZY45864.1"
                     /translation="MGLIRLAKQARSGLTRIARSRSNRRHARRAKALLALHHGQSVQQ
                     VAQATGLSRQAIYNLQKRFLARQTDRLTERLGDGQHTGRPPQKIRAAKKIIQWLLRRS
                     PRKYGYAAEDWTVPMLHQQIQKRTGAEVSRRTIRRALNELQSHS"
     gene            658..915
                     /locus_tag="VFF70_14085"
     CDS             658..915
                     /locus_tag="VFF70_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45865.1"
                     /translation="MSEQMIQHAQAVKRQHEADLLRKPNVVGVGIGFRTRGGKSTNEV
                     CIVVSVKIKVAPAHLKREELIPSSIEDVPVDVIVTGEIRAL"
     gene            938..1960
                     /locus_tag="VFF70_14090"
     CDS             938..1960
                     /locus_tag="VFF70_14090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HZY45866.1"
                     /translation="MELQLLQIAINLALQARRQYEEYLMQMNNVVATGVGFKIAGSTL
                     TTEPSIIVSVSKKLPLNQLSSADLVPKTVGDVKTDVIETGKIVAFQNLKQRMRPAQPG
                     VSIGHYQITAGTFGCLVKRGSDIFILSNNHVLANSNNAQVGDPIWQPGRFDNGSSADQ
                     IAKLEQFIPIGFTGDTTTPPPNDRPGCAPLAGILSLFAPKPASQPVTINQPGNNTVDC
                     AIAKPLTTDLVTSNILSIGIPIGVGNATLGTPVQKTGRTTGYTTGQISQIDVTVTVDY
                     DGQAATYRNQLMAGPMSAGGDSGSAVLDMNKRVVGLLFAGSDTSTIMNPFQAVLNALE
                     VQLVTS"
     gene            2086..3297
                     /locus_tag="VFF70_14095"
     CDS             2086..3297
                     /locus_tag="VFF70_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45867.1"
                     /translation="MRFRITLLLIGLVVSAACTKTGTPTPAPATSAPAAPTVAPGVPT
                     TAPAPTQQPAPKVLPRPLYFIGPTNTVNQVWRLGTDGVTLAQISHEQAEVINFDVSPA
                     DGSIAFVSSNKLIKVDANGGNRVELFTGPAINPTPGPDQINTQVSSVHWKPDGTLIAY
                     ALNGVNTIAANGGAPIIVQANDPIPSPSPTSPARFFWPAAWSPDGSRILMNVSFYPEG
                     GSLAVKIVAGGAPIMFNTPEGWPCCNPDWSLDSSAIFFANDYPGLIPAGLWRVDAASG
                     LGSTLIEGANQTGTAFNLVSQVKQLSDQRLYYFIAHANAMPDNQPAVSMTSSTVDGNT
                     DRKQLRTDSYVIGEALWDPNQTGAVIKDLTGAPLDFSGGQSTQLIWLNVNNTVPLKLP
                     AFGFNLRWGKP"
     gene            3423..3971
                     /locus_tag="VFF70_14100"
     CDS             3423..3971
                     /locus_tag="VFF70_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZY45868.1"
                     /translation="MTQHETHTVKIGALTRHLPLFQVAPGVKIALFNMLGDTEVVQTA
                     AKALAKRLPKTAQVLITAEAKSIPLAYALSVETQLPYVVLRKTYKPYMGQTIKADTLS
                     ITTGEPQTLYLDEKDIELITGKNVIIVDDVISTGSTLQGMRMIIEKAGGTICATAAVF
                     TEGERAKWKDIIALGHLPIFTA"
     gene            3979..4485
                     /locus_tag="VFF70_14105"
     CDS             3979..4485
                     /locus_tag="VFF70_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZY45869.1"
                     /translation="MPPFTREMIEQLIAERKARGHGDLFLTGAVLTELDLSGLDLRGA
                     NLRKAVLNHTDLHESIMSGVDLVEADLTGCDLRKIDLYQANLTGANLTGANLTFAHLG
                     LANLTAALLHGANLTFADAHRADFRLADLSQCNLREAKLHGANLDQADLTDAKLQGTI
                     MPNGSKHS"
     gene            4610..5254
                     /locus_tag="VFF70_14110"
     CDS             4610..5254
                     /locus_tag="VFF70_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY45870.1"
                     /translation="MAFNFLNRKDQEESVKKMGRLPPGQALTQKFPVLHYGPVPPFNP
                     ATWNFKVFGLVAEEKVWTWDEFNQLPRRKMQMDIHCVTRWSKFDTVWEGVHLKDLIDQ
                     GFIQPKPAAKFIMQHCEYNFTVNVPIDVALSDVFILATHLDGQPIEADHGYPLRGVAG
                     ALPAQPQSDHDRYLWKGGKWLRGLEFMAQDRAGFWEQAGYSMSANIWREERYGW"
     gene            complement(5308..>6398)
                     /locus_tag="VFF70_14115"
     CDS             complement(5308..>6398)
                     /locus_tag="VFF70_14115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HZY45871.1"
                     /translation="LGLIAVVWRTQASAPGQAYVPSGIENPRKWFILSWLIGLVASFI
                     FKARVVADIKPSYAVTIVLASVLVLSGGLWCLRWFSNKIVQDWPHARLDAPAVLPLRW
                     PPNWWVVWSIVGGVLTAALAFGLEIYGLKLFAPLIASATERILLAPSGSGLNDPAVVI
                     LIILGIAIVAPLIEELCKALALLLFRKSIDRPIDGLMLGMAIGIGFGLVESAFYLVGL
                     INAWWIAAWLRLATILMHGTTTSIIGRAYALSRRTGRRRDLIAGYLRAVLLHGLWNGL
                     VIGAALIALNGQAILGCGGLIFWLLLMSRQLPRIATAGVEASVQDEYAAAEQPLPSEW
                     SPLDNGLAWKLVGSEPKYYNPVTKEQLQ"
BASE COUNT         1547 a         1843 c         1669 g         1339 t
ORIGIN      
        1 catggctttg acttcttgcg ggcgagtaaa ttggcgtgcc ttctcaaata aatccatgct
       61 gcacctcctg aaacaacgag ctaagatttc tcgatggccg attgtagccc cgcttcgata
      121 aatggtcaag cgctgctgga cagaatattc ttgacaaatc attcaggaca gcttatgcta
      181 tacgtgaagg tcaggaggtt gcaggcatgg gtttaatccg attagctaaa caagcccgat
      241 ccgggttgac gcggatcgcg cgatctcgat cgaaccggcg gcacgcccgg cgcgccaaag
      301 cgttgctggc gctgcatcac ggtcaaagtg tgcagcaagt ggcgcaggcc acgggcctaa
      361 gccggcaagc gatttataat ctccagaagc gcttcctggc gcgccagacc gatcgattaa
      421 cggaacgcct cggcgacggg cagcataccg gccgcccacc ccaaaaaatt cgtgcggcca
      481 aaaagatcat tcaatggctg ctgcgccgca gtccacgcaa gtatgggtat gccgctgaag
      541 attggaccgt gcccatgctg catcagcaaa ttcaaaagcg caccggcgca gaggtcagcc
      601 gccgcactat tcgacgcgcg ttaaatgagt tacaatcgca ctcatgaggt ggcagccatg
      661 tccgagcaaa tgattcaaca tgcccaagcc gtgaagcgcc agcacgaagc tgacttgctg
      721 cgcaagccca acgtcgtcgg cgtgggcatt ggctttcgaa cccgcggcgg caaatcgacg
      781 aatgaagttt gcatcgttgt gtcagtgaaa attaaagtcg cccctgcgca cttgaaacgc
      841 gaagagttga ttccgtcatc gattgaagac gtaccggtag atgtcatcgt tactggcgaa
      901 attcgcgcgc tgtaattatt cacaggagaa ccccagcatg gaactgcaac tattgcaaat
      961 tgccattaac ctcgcccttc aagcccgccg tcagtatgaa gaatatttga tgcagatgaa
     1021 caacgtcgtc gccaccggcg tcggcttcaa gatcgcgggc agtacgctaa cgactgaacc
     1081 atccatcatc gtctcggtgt cgaagaagct gcccctcaat caactttcct cggccgactt
     1141 ggtgcccaag accgttggcg atgtgaaaac cgatgttatc gaaacaggca agatcgttgc
     1201 ctttcaaaac ctcaagcagc gtatgcgccc cgcgcagccc ggcgtcagca tcggccatta
     1261 ccagatcact gccggcactt tcggctgttt ggtgaaacgc ggcagcgata tatttatctt
     1321 gagcaacaat cacgtcttgg caaattccaa caacgcccaa gttggcgatc cgatctggca
     1381 gccaggcaga ttcgataatg gatcaagtgc cgatcaaatt gccaagctgg agcaattcat
     1441 cccgatcggt tttacaggcg acacgaccac gccacccccc aacgatcgac cgggctgtgc
     1501 gccattggcc ggcattctga gtttgtttgc gcctaaacca gccagtcagc ccgtcacgat
     1561 caatcagccg ggcaacaaca cggtcgattg cgccatcgct aaaccgttga caactgatct
     1621 ggtcacctca aatattttga gcatcggtat cccgatcggg gttggcaatg ccacgctcgg
     1681 cacgcccgtg cagaagaccg gtcgcaccac aggttacacc accggccaga tctctcagat
     1741 cgatgtgacg gttaccgttg attacgatgg acaagcggcc acttaccgca atcaattgat
     1801 ggccggcccg atgagcgcgg gcggcgattc gggatcggcg gtactggaca tgaataaacg
     1861 tgtcgtcggc ttgctcttcg ccggatcgga cacctccacg atcatgaatc cgtttcaagc
     1921 cgtgctcaat gcactcgagg tgcaattggt gaccagctaa atgagttgaa gcctcgttga
     1981 attgttgtcc aaattctcaa tgttgtataa tcgaaatgcc accgtgaaac ttgcggtggc
     2041 acttctgcat catcaagcca ccatcatttc acacaggaga cttttatgcg ttttcgaatt
     2101 acccttttac tcattgggct ggtcgtgtca gcggcttgta ccaagactgg cacgcctacg
     2161 cccgcaccag caacgagcgc gcccgccgca ccgactgttg caccgggtgt gccaacaacc
     2221 gcacccgctc cgactcaaca gcccgcgccc aaagtgctgc cgaggccatt gtattttatt
     2281 ggacccacca acacggtcaa tcaagtttgg cgattaggca ccgacggtgt cacgttggca
     2341 cagattagtc acgaacaggc tgaagtcatc aacttcgatg tctcacctgc cgacggatcg
     2401 attgcgtttg tcagcagcaa caaattgatc aaggtcgatg caaatggcgg caatcgagtt
     2461 gaactgttta ccggcccggc gatcaatcct acgcccggcc ccgatcaaat caacacgcaa
     2521 gttagctcgg tgcattggaa gcccgatggt acactgattg cttacgcgtt gaacggtgtg
     2581 aatacgatcg cggcgaatgg cggcgcgccg atcattgtgc aagccaatga tcctattccg
     2641 tcgcccagtc caaccagccc ggcaagattc ttctggcccg ctgcctggtc gccagatggt
     2701 tcacgcattt tgatgaacgt ctcgttctat ccggagggcg gcagcctggc cgtcaaaatc
     2761 gtagcgggcg gcgcgccgat catgttcaat acaccggagg gctggccgtg ctgcaatcca
     2821 gattggagtt tagatagctc agccatcttc ttcgccaacg attatcccgg cttaattcca
     2881 gcgggcttat ggcgcgtcga tgcagcctcc ggactaggct ccacactgat cgagggcgca
     2941 aatcaaaccg gtaccgcatt taatctcgtg tcacaagtca agcagttgtc cgatcaacga
     3001 ctatactatt ttatagcgca cgcgaatgcg atgccggaca accagcctgc tgtatcgatg
     3061 accagctcaa cggttgatgg caacaccgat cggaagcagc tgcgcacgga cagctatgtc
     3121 atcggcgagg cgttgtggga tcccaatcag acgggcgcag tgatcaaaga cctcaccggc
     3181 gcgccgctcg atttttccgg tggccaatcg acgcagctca tctggctgaa tgttaacaat
     3241 accgtgccgc tcaagttacc ggcctttggc ttcaatctgc gctggggcaa accgtaaaag
     3301 cccgcctgaa cgttggactt ggaataaact tttagaacct tgcagtcagg ttgatgcagg
     3361 gttctattct tgataagttg tgctagaata atacaaggtc gatctacggc cggaggctga
     3421 ttgtgactca acacgaaaca cataccgtta agatcggcgc gctcacgcga catctaccgc
     3481 tgtttcaagt cgcgccgggt gtaaagatcg cgctcttcaa catgctgggc gatactgagg
     3541 tggtgcaaac cgccgccaaa gcgctggcga aacgactgcc gaaaactgcg caagtattga
     3601 tcaccgccga agccaagagc atcccgctgg cgtatgcgtt gtcggtcgag actcaattgc
     3661 cctacgtggt gctgcgcaag acgtacaagc cgtatatggg ccaaacgatc aaagccgata
     3721 ccttatcgat cacgaccggc gagccgcaga cgttgtacct cgacgaaaaa gacattgagc
     3781 tcatcacggg caaaaatgtg atcattgtcg acgacgtgat cagcaccggc agcaccttac
     3841 agggaatgcg catgatcatc gagaaagccg gcggcaccat ctgcgccacg gcggccgtct
     3901 tcactgaagg cgaacgcgcc aagtggaaag atatcatcgc gttgggacat ctgcccatct
     3961 tcactgcgtg attgttctat gccaccattc acccgggaaa tgatcgaaca attgattgcc
     4021 gagcgcaagg cgcgtggtca tggcgatctg ttcctcactg gcgctgtttt gaccgaactc
     4081 gatctgtccg gcttggatct gcgcggcgcg aatctgcgca aggcggtcct caatcacacc
     4141 gatctacacg aatcgattat gagcggcgtg gatttagtcg aagccgatct taccggctgc
     4201 gatctacgca aaatcgatct gtatcaagcc aatttaaccg gcgccaatct caccggcgct
     4261 aatctcacct ttgcccattt gggtttggcc aatctgacgg ccgccctttt gcatggcgcg
     4321 aacctcacct ttgccgatgc gcaccgcgcc gatttcaggc tagccgacct cagtcagtgc
     4381 aacctgcgcg aagccaaact acacggtgcc aatctcgatc aggccgacct cacggacgcg
     4441 aagctgcaag gcacgatcat gccgaacggc tccaaacact cttgacctct ttccattgtt
     4501 atatttgacc cgcaggctgg gctaatgagt gccgccgatc ggatggttgt taattctttc
     4561 gtaagatcgg atggtataat ttctacagtg gtatcttgag aggagcagta tggcttttaa
     4621 ttttctgaat cgcaaagatc aagaagagtc cgtcaaaaaa atggggcggc tgcccccggg
     4681 tcaggccctg acccaaaaat ttccggtcct gcattacggg ccggtgccgc cgttcaatcc
     4741 tgccacgtgg aattttaaag tgtttggctt agtggccgaa gagaaagtct ggacgtggga
     4801 cgaattcaat cagttgccgc gccgcaagat gcaaatggac attcactgcg tcacgcgctg
     4861 gagcaagttc gatacagtct gggaaggcgt gcatcttaaa gacttgatcg atcaaggctt
     4921 tatccagccc aaaccggcag ccaaattcat catgcagcac tgcgaatata acttcacggt
     4981 caatgtgccg atcgatgtcg cgctgagcga tgtatttatt ctcgccacgc atctcgacgg
     5041 acagccgatt gaagccgatc acggttaccc gctgcgcggc gtggcgggtg cgctgcctgc
     5101 gcaaccgcag tccgatcacg atcgatatct gtggaaaggc ggcaagtggc tgcgcggcct
     5161 ggagttcatg gcgcaggatc gcgcaggctt ctgggaacag gcaggttatt caatgagcgc
     5221 gaatatctgg cgcgaagaaa gatacggttg gtaaaacaaa aaaaggtctc acagtgagac
     5281 ccgctttact tttcatgctt gatcaattta ctgcagttgt tctttggtca ctggattata
     5341 gtacttcggc tcactcccta ctaacttcca cgctaaaccg ttatccagcg gtgaccattc
     5401 agacggcaga ggttgttcgg ctgcagcata ttcgtcttgt actgacgctt caacaccggc
     5461 cgtagcgata cgcggcaatt ggcgcgacat caagagcagc cagaagatca atccgccgca
     5521 gcccaagatc gcttgaccat tcaaggcgat caaggctgca ccgatgacta aaccattcca
     5581 caggccatgc agcagcacgg cgcgcagata gcccgcgatc agatcacgcc gccgaccggt
     5641 ccggcggctg agcgcatacg cgcggccaat aatactggtg gtggtgccat gcatcaagat
     5701 cgtcgccaga cgcagccagg ccgcaatcca ccatgcattg atcaagccga ccagataaaa
     5761 cgcactttcc accaggccga agccgatacc gatggccatg cctaacatca agccatcaat
     5821 cggccgatcg atcgattttc ggaagagcag caaggctaac gctttgcaca gctcttcgat
     5881 caacggcgcg acaatcgcta tccccaaaat gatcaatatg accaccgctg gatcattcaa
     5941 gccgctgccg gatggcgcga gcagaattcg ttcagtggcc gaagcgatca acggcgcgaa
     6001 taatttaagg ccataaatct caaggccaaa cgccagcgct gcagtcaaca ctccacccac
     6061 tatcgaccag accacccacc agttgggcgg ccagcgcagc ggcaggaccg cgggcgcgtc
     6121 cagtcgagcg tgtggccaat cctgcactat tttgttgctg aaccagcgca agcaccacaa
     6181 cccgccgctc aacaccagca ccgaggccaa tactatcgtc acggcatacg acggtttgat
     6241 gtcggccaca actcgagctt taaaaataaa cgaggccacc agaccgatca gccaggacag
     6301 gataaaccac ttgcgtggat tttcgatccc gctgggcaca taagcctggc cgggcgcaga
     6361 ggcttgggtg cgccacacaa ccgcgattag ccctaacc
//
LOCUS       DASRTJ010000698         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_143981,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000698 DASRTJ010000000
VERSION     DASRTJ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143981"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFF70_14120"
     CDS             <1..694
                     /locus_tag="VFF70_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZY45872.1"
                     /translation="LTSIQGFSQAIKDGMTNDPETIRRSAAIIFDESNRMGRMVSELL
                     DLARIESGQIVMRSEPVKIDQLVLSVVEKMALRAQQSSIALDAQVPMDLPSINGDGDR
                     LAQVFSNLIDNALKHTPAGGKVTVSARSLSGSSVMRRGKPWPGGVEVIVADTGSGIPP
                     EDLARIFERFYQVDKSRARSSGGGSNLGLGLAIVKHIVVGHGGSIHAESVVGLGTKFV
                     VTLPIARGAKPN"
     gene            880..1152
                     /locus_tag="VFF70_14125"
     CDS             880..1152
                     /locus_tag="VFF70_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45873.1"
                     /translation="MTTFSPADHIIYAAIELSRIETGLTAKVVWTAVDTSAGQNIEVA
                     QKEFTSLAVNVIQAQVELPQDWPTGKYKLDVYLNGTLAKTVEFTVQ"
     regulatory      1517..1615
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1684..>4497
                     /locus_tag="VFF70_14130"
     CDS             1684..>4497
                     /locus_tag="VFF70_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZY45874.1"
                     /translation="MQIFPHTNRKYLDALTDHVVVFDGAMGTSVQACNLHAHDFGGEH
                     LNGCNDYLVITKPDVIEQIHASFLAVGCEVIETDTFRANRITMREYKLQDQIIAINRA
                     AASLARHVADRFASETGIPRFVAGSIGPSGLLPSTNDPTLSNITFEELVDVFREQSVG
                     LIEGGADLLLIETQQDILETKAAVHGAWRAFEETGKRIPVQTQITLDTSGRMLLGTDI
                     AAALTTLEALPIVDVIGLNCSTGPDYMREPVRYLTTHSRKPISVIPNAGLPLNVDGQA
                     VYPMKPVPLAETLAEFVTELGVSVVGGCCGTTPEHLKEVIDRVRGLKPKVREIEYEPM
                     ISSAMRAIPLHQDPAPLIVGERVNSQGSRKIKKMLLEDDYDGILTVARDQVEGGAHML
                     DVCVALTERADEGAQMKAVVKKLQMSVESPLMIDSTEADVIKTALEMNPGRAIINSIN
                     LENGRKRCDAVLPLAVAHGAAVIALTIDEQGMAKTSDAKLEVAKRIYDIAVNEYGLSP
                     DSLIYDTLTFTLATGDNEMAGSAIETMDGIRKIKAELPGVLTSLGVSNVSFGLNPAAR
                     SAINSVFLHHCVQAGLDMALVHPKDITPYAEIDPFVRSLCDDLILNRAPDALTKLIQH
                     FENVKVDASKPIEDPTEGLSVEQKIHWMIVHRKKEGIEPLIDQSISAHGTTPNDGAVW
                     TLNSVLLPAMKEVGDKFGAGELILPFVLQSAEVMKRAVAHLEKSLDRKEGTTKGRVVL
                     ATVYGDVHDIGKSLVNTILSNNGYTTFDLGKQVPANVIIDKAIEVNATAIGLSALLVS
                     TSKQMPLIVNELQRRGLQYPVLIGGAAINRRFGRRILFTESGAPYEPGVFYCKDAFEG
                     LDAMEKLIDPNERGDFVGRIITEAEAEVKGAATADRRPQTAVDQPRSSVSRLRSADIP
                     QPPFWGSRVIREMRLEIVLKHLDI"
BASE COUNT         1056 a         1137 c         1327 g          977 t
ORIGIN      
        1 gctgaccagc atccagggtt tttcccaagc gatcaaagat ggtatgacga atgatcctga
       61 aacaatccgc aggagcgcgg cgatcatttt cgacgaatcg aatcgcatgg gccgcatggt
      121 gagcgagttg cttgatttag cgcgcatcga gagcggtcag atcgtcatgc gcagcgagcc
      181 ggtcaagatc gatcaactgg tgctgagcgt ggtcgagaag atggcactgc gtgcgcagca
      241 aagcagcatt gcgttggatg ctcaagtccc gatggatctg ccatcgatca acggggatgg
      301 agatcggttg gcgcaagtct tcagtaactt gatcgataac gcgttgaagc acacgcccgc
      361 tggcggcaaa gtcaccgtct ctgctcgatc gctgtcaggt tcatcggtta tgcggcgggg
      421 caagccatgg cccggtggcg ttgaagtgat cgtcgcagat accggctcgg gcattccgcc
      481 tgaagatctc gcgcgcatct tcgagcgctt ttatcaagtg gataagtcgc gcgctcgatc
      541 gagcggaggt ggcagcaatt taggtttggg actcgcgatt gtgaagcaca ttgtcgtcgg
      601 gcatggcgga tcgatccatg cggagagcgt tgttgggttg gggacaaaat ttgtggtgac
      661 gctgccgatc gcgcgcggag caaaacccaa ctaattgact ttcaggatgc gcattgatac
      721 aatgctttca tcttaaagat tagaggttac acgatgagac cctttacatt tctatttatt
      781 gctttgcttg ttacgagtat gaccttgtca gcctgtggtg ctgctggccc gctcactgta
      841 caaaacgtta cactgtctag cacgtcaggc ggaacagcag tgacgacttt cagcccggca
      901 gatcacatca tctacgccgc gatcgaactg agccgcattg aaacgggtct gacggccaag
      961 gtggtgtgga cggcggtcga taccagtgcc ggtcagaata tcgaggtcgc ccagaaggaa
     1021 tttacgagct tagcagttaa tgtcattcag gctcaggtcg aattgccaca agactggcca
     1081 acgggcaagt acaagttaga cgtctatctg aacggcacgc tcgctaaaac agttgagttc
     1141 actgtccaat aatcgtggtg ggtggtgcgg ctcggccgcc cggaagaacc atcttaatgg
     1201 ttcgcgatgt cccttctgat cagaagggtc attttgtttc tgaagactat cagcgccgtc
     1261 gcggggcgga tcgatcaagg cggagagcgt cgttggcgtg ggggcctgat tcatcgtgtt
     1321 gctgccgatc gatcgaaata agggataagg gcagagggat gaaggatgaa agaaagcatc
     1381 ctgagcggag cgatgatctc gaagagagca tcgcgctgtc gaaggagcga atcaacgaat
     1441 cagcgataag ccatctgcta tcggctattg acagatcaac accctgcggg tatatttaat
     1501 ccacaatcca atatcattct catccagaga ggcggaggga ccggcccgac gaagcctcgg
     1561 caaccaagga aatcctatgg tgccaaatcc ggcagaaaat tctggaagat gaggagcagt
     1621 cccgcttttg acaatgcctc ttctaaccag ttgaggcatt ttattttttt acaggacgtc
     1681 gacatgcaga tttttccgca caccaatcga aaatacctcg atgctctcac cgatcacgtg
     1741 gtcgtgttcg acggtgccat gggcaccagc gtgcaggcct gcaacttgca cgcgcacgat
     1801 ttcggcggtg agcatctcaa cggctgcaac gattatctgg tcatcaccaa gccggatgtc
     1861 attgagcaga ttcacgcctc gttcctcgcc gtcggctgcg aagtgatcga gacggatact
     1921 tttcgcgcga accgcatcac aatgcgcgaa tacaaactac aagatcaaat tattgcgatc
     1981 aatagagcgg cggcttcatt ggctcgtcat gtcgccgatc ggtttgccag cgaaacgggc
     2041 ataccgcgct tcgtcgccgg atcgatcggg ccgtccggcc tgctgccttc gacgaatgat
     2101 ccaacactaa gcaatattac ctttgaagaa ttggtcgatg tgtttcgtga acaatctgtc
     2161 ggcttgatcg aaggtggcgc ggacttgctg ctgattgaaa cgcagcagga cattctcgaa
     2221 accaaagccg cggtgcatgg cgcgtggcga gcgtttgaag aaacgggcaa acgcattccg
     2281 gtccaaacgc aaatcacgtt ggatacttcg ggacgcatgt tgctcggcac ggacatcgcg
     2341 gcggcgttga ccacacttga agcactgccg atcgtcgatg tgattggctt gaattgttcg
     2401 accgggcctg attacatgcg cgaaccggtg cgatatttga cgacgcattc acgcaagccg
     2461 atcagtgtga tcccgaacgc gggcttgccg ctcaacgtcg atgggcaggc cgtgtacccg
     2521 atgaagcccg tgccgctcgc cgaaacgttg gctgagttcg tgaccgagtt gggcgtaagc
     2581 gtcgtcggtg gttgctgcgg cacgacaccg gaacatttga aagaagtaat cgatcgtgtg
     2641 cgcggcttga aacccaaagt tcgcgagatc gaatacgaac cgatgatctc atcggcgatg
     2701 cgagcgatcc cgctgcatca agaccctgcg ccattgattg tcggtgagcg ggtcaactcg
     2761 cagggcagcc gcaagatcaa gaagatgctg ctcgaagatg attacgacgg catcttgacc
     2821 gtcgcgcgcg atcaagtcga gggcggcgcg cacatgctcg atgtgtgtgt ggcgttgacc
     2881 gagcgcgccg atgagggcgc gcaaatgaaa gcggtcgtca agaaattgca gatgagcgtt
     2941 gaatcgccgt tgatgatcga ttcgaccgaa gcggatgtga tcaagaccgc gctcgaaatg
     3001 aatcccggcc gcgcgatcat caactcgatc aatctggaga acgggcgcaa gcgctgtgat
     3061 gccgtgctgc cgctggccgt cgcacatggc gcggcagtga tcgccttgac gatcgatgag
     3121 cagggcatgg cgaaaacatc cgatgcgaaa ctggaagttg ccaaacgcat ctacgatatc
     3181 gctgtgaatg aatacggcct gtcgcccgat tcattgatct acgacacctt gacgtttacc
     3241 ctcgcgaccg gcgataacga aatggcgggc agcgcgatcg aaacgatgga cggcatccgt
     3301 aagatcaagg ctgagctgcc gggcgtgttg acttcgttgg gggtcagtaa tgtctcgttc
     3361 ggcttgaatc ctgccgcacg ttcggcaatt aacagcgtgt tcctgcatca ctgcgtgcaa
     3421 gccggactcg atatggcgct ggtgcacccc aaagacatca cgccgtacgc cgagatcgat
     3481 cccttcgtcc gatcgttgtg cgacgatctg atccttaatc gcgcgccgga tgcgctgacg
     3541 aaattgattc agcactttga aaacgtcaaa gtggatgcca gcaaaccgat cgaggatccc
     3601 accgaaggcc tgtccgtgga gcagaagatc cattggatga tcgtacatcg caagaaggaa
     3661 ggcatcgaac cgctgatcga tcaatcgatc agcgcgcatg gaacgacacc caacgatggc
     3721 gcagtctgga cattgaacag tgtcttgctg cccgcaatga aagaagtcgg cgataaattt
     3781 ggcgcgggcg aattgattct gccgttcgta ctgcaatccg ccgaagtaat gaagcgcgca
     3841 gtcgcgcact tggaaaagtc gctcgatcga aaagagggca caaccaaagg ccgtgtcgtg
     3901 ctggcgaccg tgtatggcga tgtgcatgac atcggcaaga gtttggtgaa tacaatttta
     3961 tccaacaacg gctacaccac tttcgatttg ggcaaacaag ttccagcgaa tgtgatcatc
     4021 gataaagcga tcgaagtgaa tgcgaccgcg atcggcttga gtgcgctgtt ggtgtcaacg
     4081 tcgaagcaga tgccgttgat cgtcaatgaa cttcagcggc gcggcttgca gtatccggtg
     4141 ttgatcggcg gtgcggcgat caatcgacgc ttcgggcgca gaattttatt tactgaatca
     4201 ggcgcaccgt atgagccggg tgtgttctat tgcaaagatg cgttcgaagg tttggatgcg
     4261 atggagaaat tgatcgatcc gaatgagcgc ggtgattttg tcgggcgcat tattacggaa
     4321 gcggaagcag aagtaaaagg tgctgcgacc gccgaccgta gaccgcagac cgcagtagat
     4381 cagccgcggt catcggtcag ccgtctgcgg tctgctgata ttcctcagcc gccattctgg
     4441 ggctcgcgcg tgattcgtga gatgcggctt gaaattgtgc tgaagcatct ggatatc
//
LOCUS       DASRTJ010000699         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_371788,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000699 DASRTJ010000000
VERSION     DASRTJ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371788"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VFF70_14135"
     CDS             complement(<1..1573)
                     /locus_tag="VFF70_14135"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZY45875.1"
                     /translation="MKRTEVTPIVLAVLIGLLMLLAAVDLLSKPESVSASHVEQTSSG
                     GTICFANRDSVSCVQRLGVQSIGSITDQVRSLIQSMLSGPTFSERAAGVQSALPDRSK
                     LGDLAVTPDRVVIDFNLPSDFIASLRDDQVEAVNEQIMVTLTPYNFTRVEINALAVDG
                     TYQLLSSFLKPIVIPQKQSSIQPSPVTTGEGVLAGQPPINGQPQSHGGLVGKTVFVSA
                     GHGWYYNTSLGGYYTQRPTYPSSPYPAGEGIVEDFNNAEVVNQYLLQYLWNAGADAWT
                     VRERDLNTSMLIVDDSSASFTTQGSWLSGANGYAGSYHMASTVNSAATATATWTFTPS
                     ITNTYAVYIWYPSPVVTRTADAHYTIEHAGATTPVTINQTRDADNWRFLGMYPFYGGQ
                     PARIRLTNQSVISGLSVIADAIRIGGGLGDVSVAGAPPSGKPRWEEQSRQYAKWVGMP
                     DVDNLNDVIVRPLYSEWEKESGEDSVYVSWHTNGFNGYNTTASGTESYVYLSPTAGSD
                     LLQDSIHTALLNGLHT"
BASE COUNT          428 a          449 c          437 g          391 t
ORIGIN      
        1 gagtatgaag gccattcagc agcgcagtgt gaatagaatc ttgtaaaaga tcactgcccg
       61 ccgtcggcga caagtacaca tacgattcgg tcccactcgc ggtcgtgttg taaccattga
      121 aaccattggt atgccacgac acgtacactg aatcttcgcc cgactctttt tcccattccg
      181 aataaagcgg cctgacgatt acatcgttga gattatcaac atcaggcatg ccgacccact
      241 tcgcatattg cctcgattgt tcttcccagc gcggtttgcc acttggcggc gcgccggcca
      301 ccgatacatc accaaggccc ccgccgatac gaatggcgtc tgcgatcaca ctcaagccac
      361 tgatgaccga ttgattcgtc aagcgaatac gcgccggttg tccaccataa aatggataca
      421 tacccaggaa acgccaattg tcggcatcac gcgtttgatt aatcgtaacc ggcgttgtcg
      481 ctccggcatg ctcgatagtg taatgcgcat cggcggtgcg cgtcacaacg ggagacggat
      541 accagatata caccgcataa gtattggtga tcgatggcgt aaatgtccat gtcgcagtcg
      601 ctgttgctgc tgaattcacc gtcgatgcca tatgatagct gccagcataa ccgttcgcgc
      661 cggagagcca actgccctgt gtcgtgaatg acgcgctgct gtcgtcgaca atcaacatcg
      721 acgtattcaa atcgcgttca cgcactgtcc aggcatcagc cccggcattc cacaaatatt
      781 gcagcaagta ttgattgaca acttcagcat tgttgaaatc ctcgacaatg ccttcgccgg
      841 ctggatatgg cgaacttgga taagtggggc gctgagtgta atagccaccc agactcgtgt
      901 tgtaatacca accatggcct gcgctgacga acacagtttt accaaccaat ccgccatgcg
      961 attgaggctg tccgttaatt ggcggctgcc cggccagaac cccctctcca gtagttacag
     1021 gggagggctg gattgaggat tgtttttgtg gaatgacaat cggctttaaa aaggaggaaa
     1081 gtaattgata agtgccatca accgcaagcg cattgatttc aacacgcgtg aagttgtacg
     1141 gcgtcaaggt gaccatgatc tgctcgttga ccgcttcaac ttgatcgtcg cgcagtgagg
     1201 cgatgaagtc agagggtaga ttaaaatcga ttacgacacg gtcgggcgtg actgcgagat
     1261 cgcccaactt cgatcgatcc ggcagcgcgg attgaactcc ggccgcccgc tcggagaaag
     1321 ttgggccgga taacatcgac tggatcagcg aacggacctg atcggtaatc gagccgatcg
     1381 attgcacgcc gagccgttga acgcagctaa ctgaatctct attcgcaaaa cagatcgttc
     1441 caccggatga tgtttgttcg acgtgagaag cggagactga ttccggcttg ctaagtaagt
     1501 caactgccgc cagcagcatg agcaggccga tgagcaccgc caaaacgatc ggggtcacct
     1561 cagtacgttt catgatcgaa cctcgtgttt attatagcaa aatagttcga gtatggtata
     1621 tcgctaagtg ctggtctata ttgaaaaggg tccattcata atcgtaaaac gaaagtcttg
     1681 cggaggatta tctcctccgc aagac
//
LOCUS       DASRTJ010000700         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_371818,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000700 DASRTJ010000000
VERSION     DASRTJ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371818"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1813)
                     /gene="lepA"
                     /locus_tag="VFF70_14140"
     CDS             complement(11..1813)
                     /gene="lepA"
                     /locus_tag="VFF70_14140"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZY45876.1"
                     /translation="MDEKHIRNFCIIAHVDHGKSTLADRLLEHTGTISLREMKEQVLD
                     SMELEREKGVTIKASAVRMRYTAQDGQEYEMNLIDTPGHVDFTYEVSRALNACEGALL
                     VVDATQGVEAQTLANMYLAIDANLTIIPVINKIDLDSARPDDAAAEIAHLLGISPVDL
                     IRASAKEGIGIDEILEAIVAQVPEPRGNPETSLRALIFDTHYDAFKGVVAYLRVIDGS
                     IDRTTKIKLMATNVEAEPLEIGTFSPTMKPSDRLEAGEVGYIATGLKTIRDCHVGDTI
                     TDANNPTSEPLPGYRPVKPMVYAGLYPVEADDYQVLKDALEKLQLNDASLIYEPETSQ
                     ALNFGFRCGFLGLFHMEIIQERVEREYDLDILMTAPSVEYEVLLSNGNSIVVDSPAQL
                     PDPSTIDEIREPWMNVQIFTPTEYIGTIMDLVINRRGAQKDMEYLDPQRVMITYELPL
                     AELIIDFHDQLKSRTRGYASMDYTLVGYRAGDLVKMDILVNEIVVDALATIVHRKAAY
                     DRGSKITSKMKELIPSQMFPVPLQAAVGGKIIARSTVKAQRKDVLAKCYGGDISRKRK
                     LLEKQKAGKKRLKMVGSVEVPQEAFMAILKMGQD"
BASE COUNT          419 a          556 c          478 g          468 t
ORIGIN      
        1 atgcttagat tcagtcttgt cccatcttta agatcgccat gaatgcttct tgcggcactt
       61 cgaccgagcc gaccattttc agccgcttct tgcccgcctt ttgtttctcc agtaatttgc
      121 gcttgcggct gatgtcgccg ccatagcatt tggcgagcac atctttgcgc tgtgccttca
      181 cggtcgatcg agcgatgatt ttgccgccga ccgcggcttg cagcggaacc ggaaacattt
      241 gagacgggat caattccttc atcttcgaag tgatcttgct gccccgatcg tacgcggctt
      301 tgcggtgaac gatcgtcgct aaggcatcga cgacgatttc attgacgaga atatccatct
      361 tcaccagatc gcccgcgcgg taaccgacga gcgtgtaatc catcgaagcg taaccgcgcg
      421 tgcggctttt cagttgatcg tgaaagtcga tgatcaattc agccagcggc agttcatagg
      481 tgatcatcac acgctgcgga tcgagatact ccatgtcctt ctgcgcgccg cgccgattga
      541 tcaccaagtc catgatggtg ccgatgtatt ctgtcggcgt gaagatttga acgttcatcc
      601 acggctcacg aatctcgtcg atcgtcgatg gatccggcag ctgcgcgggc gagtccacaa
      661 cgatcgagtt gccgttgctt aacagcactt cgtattccac gctcggcgca gtcatcaaga
      721 tatcaagatc gtattcacgc tccacgcgct cttgaatgat ctccatgtgg aacaagccca
      781 gaaagccgca gcgaaaacca aagttcaacg cttgcgatgt ttccggctca tagatcaacg
      841 acgcatcatt cagctgcaac ttttccagcg cgtccttcaa aacttgataa tcgtcggcct
      901 cgaccggata taaaccggca tacaccatcg gcttaacggg acgataaccc ggcagcggct
      961 ctgatgtcgg attgttcgca tccgtgatag tatcgcctac atggcaatcg cggatggttt
     1021 ttaagccggt agcaatatag ccaacttcac ccgcttccaa tcgatcgcta ggcttcattg
     1081 tcggtgagaa cgtgccgatc tccaacggct cggcctcgac gttcgtcgcc attagtttga
     1141 tcttggtcgt ccgatcgatc gatccgtcga tcacgcgcag gtaggcgacc acgcctttga
     1201 acgcatcgta atgcgtatcg aagatcaacg cgcgcagtga agtttcggga ttgccgcgag
     1261 gttccggcac ttgcgccaca atcgcttcca aaatttcatc gatgccgatg ccttctttag
     1321 ccgaagcgcg aatcagatca acaggcgata tgcccaacag atgcgcgatc tcggccgccg
     1381 cgtcatcggg ccgggccgaa tccagatcga tcttgttgat gaccgggatg attgtcaagt
     1441 tcgcatcgat cgccaaatac atgttcgcca gcgtctgcgc ctcaacgccc tgtgtcgcat
     1501 cgacgaccaa cagcgcacct tcacacgcgt tcaaggcacg gctgacttcg tacgtgaaat
     1561 cgacgtggcc gggtgtgtcg atcaagttca tctcatactc ttgaccgtcc tgagcggtgt
     1621 agcgcatccg cacggccgag gctttgatcg tcacgccctt ctcgcgttca agctccatcg
     1681 agtcgagcac ctgctccttc atttcgcgca aagagatcgt gccggtatgt tccaataatc
     1741 gatcggccag tgtggacttg ccgtgatcga catgcgcgat gatgcaaaaa ttccggatat
     1801 gtttttcatc cattgcgcac cgattatacc agcttaaaaa gagacttccg aagatgttgg
     1861 tcacttcgaa agtctcgttc agcgcaaact tgccgcgatc gacctacgca cagatatact
     1921 a
//
LOCUS       DASRTJ010000701        12634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_144121,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000701 DASRTJ010000000
VERSION     DASRTJ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144121"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VFF70_14145"
     CDS             <1..1323
                     /locus_tag="VFF70_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZY45877.1"
                     /translation="LDEYVGQAEIIGPNRLLRRAIETDKMFSSILLWGPPGTGKTTLA
                     MVIANTTKSHFEQINAVMSGVADIRRSVEEAHERMKLYRQRTILLIDEIHRFNKAQQD
                     ALLPHVENGTLILIGATTENPYFEVIGPLVSRSRVFQLKPLSDDNIRDLIDRAVHDPN
                     RGYGQLSIAIDPEAIEHLIRVAGGDARNALNALELAIESTPLNKENKIHLTLEVAQES
                     IQRRAILYDKDGDAHYDTISAFIKSVRGSDPDAALYWLAKMLYAGEDPRFILRRLLIL
                     AGEDIGLADPMGLVVANSAAQAFEWVGLPEGVYPIIEATIYLSTAPKSNSAGAYFRAL
                     KQVEAEGKVDVPKHLQDTNRDAKALGHGAGYQYPHSFEGHHVPQNYLPDAIKGMRFYD
                     PSNQGYESQVQDRLERWRKATEAALSIVPQAGPEITEQEIISLKKTIR"
     gene            1454..3295
                     /gene="htpG"
                     /locus_tag="VFF70_14150"
     CDS             1454..3295
                     /gene="htpG"
                     /locus_tag="VFF70_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZY45878.1"
                     /translation="MPTESHEFKAEIQQLLNILVHSLYTDRDIFLRELISNASDALSR
                     IQFEMLTNREVLDHDTELAIHIDYDKEAKTFTITDSGVGMTRDEIVENLGTIAHSGAA
                     EFLKRVQVDKKSADVIGQFGVGFYSVYMVADEVTVTSRSYRQDMQAVRWTSKGDNAYT
                     LEEAEKADRGTEIVVKLKEDALEYADQYKIQQIVKKHSDFVSFPIYVKDKVANQQTAL
                     WRLSPHDVTDDQYNEFYRQLTLDFEKPLLQIHMVTDVPVDVHALFFIPAKREPGMFSL
                     RREDGLKLYSRKILIREYFKELLPNYFRFVQGVIDSEDLPLSVSREAIQSNRALAKLK
                     STLTHKIAGELAELGEQDAAQYNAFWKEFGAFIKEGIATDASNQIELAKLLRFKTNRS
                     ADKLTSLQQHVDRMKPDQNSIYYILGEDLMSIASSPHLDYFKQHDLEVLYLVEPIDSF
                     MMLTLKEFAGRKLQSVADANLALPKSDQPVESKVVNDVYAQLVARIKNTLGEKITEVR
                     ESKVLSESPCRLVSPEESTDQEMQRVRRMLGQEYEIPKKIMEINRGHQLIADLGSLAK
                     SGSNDALLDACIQQLYESSLLIEGLLPNPAEMIPRIQTLMEAAVREK"
     gene            3382..4263
                     /locus_tag="VFF70_14155"
     CDS             3382..4263
                     /locus_tag="VFF70_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45879.1"
                     /translation="MTVSPAEIYQLVLAALHRLQDDGGQFQRAIVRKQLSDLEFWLLS
                     RLRYFPASITPGDFQTYGPYTSVANYEQSLEALTAKGLVEKVETNRYRSAETGRKLIE
                     KLYHAYFSRIAKHDFLHAAEIQRLSALADQVLNSALHQPNLPTPITQATFSVFPSNED
                     VWVMAERRMVALSAFRSDSHIAAWRDKGWSGPEVALSTALFQSADGLDHDQLRAAAVA
                     LNDKDFKSALSALSADGEISSNYDRYRLSAAGQTSRQKIEDRTNRNYSLPFNALEPVE
                     RDEIVELLEKTRGPQST"
     gene            4520..5584
                     /locus_tag="VFF70_14160"
     CDS             4520..5584
                     /locus_tag="VFF70_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZY45880.1"
                     /translation="MRIEFFLRLIGLLVFGMGGLFIGQQLVSSGGIALQDGIASIAVT
                     TLVGGLIGFILIPWFTTRPFSALQRQIRQAPAVDLAAVMAGLGFGLFIAVLTSFPLSF
                     LPTPFHEILPVISAIVFGTLGMWTLGTRRRDLAALFGNRLPTRSIEAGPPVGEERSVL
                     LDTSVIIDGRIADISQTGFVTGTMLVPRFVLNELQHIADSSDVLRRNRGRRGLDMLNR
                     MQKDSRSLLRITDMDIEEVREVDDKLVSLAKRLKCPVLTNDYNLNRVAEIQGVTVLNV
                     NELANAVKLVFLPGENIRVEVIQEGKEVGQGVGYLDDGTMVVVEEGRRFMGSTLSVTV
                     TKILQTAAGRMIFARPDNLN"
     gene            5681..7126
                     /gene="cysS"
                     /locus_tag="VFF70_14165"
     CDS             5681..7126
                     /gene="cysS"
                     /locus_tag="VFF70_14165"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZY45881.1"
                     /translation="MTIQVYNVLTHQKEEFKPLNPDLVTMYVCGPTVYDLSHIGHAKT
                     YVGFDVIVRYLRFSGYRVRYVQNITDVGHLLDTGEDRILKGVRRERIEPMELVERYTR
                     AYFADMDALGVVRPDISPRASAHIPEQIEMIKKLIADGYAYEVAGSVYFSVEKFPEYG
                     KLSGRKVEEAEEGARLGVRDEKRHPADFALWKKAEPEHILRWPSPWGWGFPGWHIECS
                     AMSTKYLGETFDIHGGGIDNIFPHNECEIAQAEAVTHQTFARYWLLTGSLSLEGVKMS
                     KSLGNTLTIQDALKRWPAQAIRYFILSSHYSNPIDFSDEAIEAAHKGLNRVLVPVRAV
                     RDRLRTATDTDEAADFKATLDQHRAALTAAMDENFNAPAALGILSQLTTDVNTLLSSG
                     QPIGQQTLAAIDQLYRDFGGTVLGIIPDEFKPESNAELLDGLVRMLTELRTEARATKD
                     FKRGDQIRDQLAELGVTLEDSRDGTTWRLNK"
     gene            7169..7915
                     /gene="rlmB"
                     /locus_tag="VFF70_14170"
     CDS             7169..7915
                     /gene="rlmB"
                     /locus_tag="VFF70_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZY45882.1"
                     /translation="MKETIYGRNAVYETLKANRRQVYQLLVAEGVQEKGNLAEALKIA
                     AQRSIPVQRVKRETLDHQTDSHQGLMAEVNSYPYAGIEDILDLAKQRHELPLILFLDV
                     IQNPQNLGTLLRTAEAIGVHGVVIPQRRGVEVTQAVVNASSGASEHLLIAQVVNLVVI
                     MTDLKQQEIWITGLDNLPEANSIEEADLSGAIGLVVGNEGEGMRRLVRENCDFLVKLP
                     MRGQMESLNAAVAGSIVLYEILRQRMRSRS"
     gene            complement(7950..8393)
                     /gene="dtd"
                     /locus_tag="VFF70_14175"
     CDS             complement(7950..8393)
                     /gene="dtd"
                     /locus_tag="VFF70_14175"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZY45883.1"
                     /translation="MRLVIQRVSSASVSVDGKIVGQCGRGLCILAGVKVGDTQAEAEW
                     LANKVANLRVFDDDEGKLNRSILDLKGEALVVSQFTLYADARKGRRPTFIDAEAPAKA
                     EPLFSHFVDRLRAEGVPVQIGVFQTIMQVEIHNDGPVTIILEKES"
     gene            8840..9262
                     /locus_tag="VFF70_14180"
     CDS             8840..9262
                     /locus_tag="VFF70_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZY45884.1"
                     /translation="MNIGNILATKGSRVITIHPEQSLKEAIALLTQHQIGALVVVDKA
                     GKPVGILTERDIVHAADRSGEAMLKHAVKDVMTKNVVIATPHDELQSVLKTMSDRRFR
                     HMPVMDEGQLAGIISIGDVVKAQLDEYRGAIDTLQSQL"
     gene            9274..10680
                     /locus_tag="VFF70_14185"
     CDS             9274..10680
                     /locus_tag="VFF70_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY45885.1"
                     /translation="MTLVGTDTNPLRVAIIGSGPAAFYAADELLKQKDLVIEIDMFDR
                     LPTPFGLVRGGVAPDHQKIKSVTKAYDGIASHPRFRFYGNVEYGQHIKLIDLKDHYHQ
                     ILFATGAQTDRRMGISGEDLDGSHPATEFVAWYNGHPDYRHLKFDLSHERVAVVGVGN
                     VAIDVARILCLTPAELAETDMADYAIDALSHSQVKEVHLLGRRGPAQAAFTNPEIKEV
                     GELEDADAITLSDEVQLDELSVTELASLNDRTLNKKVEIIQSYAHAARRSVSKARKLY
                     IRFLVSPTEMIDDGHGQVCGLKLVHNVLYKTEAGTLSPKATDQFEQLHVGLVFRSVGY
                     RGVPLPEVPFNDRYGVIMNDKGRVIEVESKQQVIGEYAAGWIKRGPSGVIGTNKPDSV
                     ETVTHMLEDLRSGSILQPVQPTVNSMADLIKQRQPQFFSYLDWKKLDELEVAKGQPLG
                     RPRVKFTSVAEMITALGK"
     gene            10725..12311
                     /locus_tag="VFF70_14190"
     CDS             10725..12311
                     /locus_tag="VFF70_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZY45886.1"
                     /translation="MKLVTVDQMRKIETAADASGHSFAQMMELAGSAVARVIQDRLDV
                     RGKRILLLIGPGNNGGDGLVAARVLKDAGADIGVYLLKARDDELIKTLRSRSVFIAAL
                     PDDRGLRFLHLWLSNCDLIIDALLGTGSARPISGDLAKLLNAVNAEVATRRAAYTSTL
                     RSLSWPGLETLAPLIVAIDGPTGLNCDTGELDPNSLSANISVTFAYPKIGHARLPGAR
                     AVGELIVADIGIDPQLAKDIELELAEAQLIRSLLPARPIDAHKGTFGKALIVAGSIEY
                     TGAPILSATAAYRSGAGLVTLATPRSIHAIISNKINEATFLPLPDQNGALSAEAVEPL
                     IEMLKTYDAVLIGPGLSLKAQSFIDSVFNSTLWPPLVVDADALTILAQIDRWWTRVPA
                     PAILTPHPGEMSRLTKIDRDQIEADRLGIARKFAAVWGQVVVLKGAFSVIAAPDGRSI
                     IMPFANPALATAGSGDVLAGVIVSMRAQGLAAYEAAIVGTYLHGLAGEIARREIGLSG
                     VLAGDLLLRLPQAIKQCSAV"
     gene            complement(12357..12491)
                     /gene="tatA"
                     /locus_tag="VFF70_14195"
                     /pseudo
     CDS             complement(12357..12491)
                     /gene="tatA"
                     /locus_tag="VFF70_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
BASE COUNT         2974 a         3363 c         3462 g         2835 t
ORIGIN      
        1 ctcgacgaat acgtcgggca ggctgagatc atcggaccga accgcttgct gcgccgcgcg
       61 atcgaaacgg acaagatgtt ctcctcgatc ctgctgtggg gtccgcccgg caccggcaag
      121 accacgctgg ccatggttat cgccaacacg accaagagtc acttcgaaca aatcaatgcc
      181 gtcatgtccg gcgtggccga catccgccgc agcgtcgagg aagcgcacga gcgcatgaaa
      241 ctgtatcgcc agcgcacgat cctgctcatc gacgagattc atcgcttcaa caaggcgcag
      301 caggatgcgc tgctgccgca cgtcgagaac ggcacgctca ttttgatcgg cgcgacaacg
      361 gagaatccgt acttcgaagt gattggcccg ttggtcagtc gatcgcgtgt gtttcaatta
      421 aagccactgt ctgatgataa tatacgcgat ttgatcgatc gggcggtgca tgatcccaat
      481 cggggttacg gccaactctc gatcgcgatc gatcccgaag cgatcgaaca tctcatccgc
      541 gtggccggtg gcgatgcccg caacgcgctc aacgcgctcg aattggcgat cgagtcgaca
      601 ccgcttaata aggaaaacaa aattcatctt acgcttgaag tcgcgcagga gtcgatccaa
      661 cgccgcgcga tcttatacga caaggacggc gacgcgcact acgacacgat cagcgcattc
      721 atcaaatcgg tgcgcggctc tgatccggat gcggcactct attggttggc gaaaatgttg
      781 tatgcgggcg aagatcccag attcattctg cgtcgattgt tgatcctcgc gggtgaagac
      841 attggcctgg ctgacccaat gggtttggtc gtggcgaatt cggcggctca ggcgtttgaa
      901 tgggtggggt tgccggaagg cgtatatccg atcatcgaag cgacgatcta tttatcgacc
      961 gcgccgaaga gcaattcggc gggcgcgtac ttccgcgcgt tgaaacaagt ggaggcggaa
     1021 ggcaaagtgg atgtgcccaa gcatttgcaa gacaccaatc gggatgccaa ggcattggga
     1081 catggcgcag gctatcaata tcctcactcg ttcgaagggc atcacgtccc gcaaaattat
     1141 ttgcctgacg cgatcaaggg catgcgtttt tacgatccca gcaatcaagg ttacgaatca
     1201 caagtgcaag atcgacttga acgctggcgt aaggccaccg aggccgcgtt atcgattgtg
     1261 ccgcaggccg ggccggaaat caccgagcag gaaattatca gcttaaaaaa gacaatcagg
     1321 tagcaggttg caagcggcat agggcaacat agccagtgat acttgatcct gtgtccctgg
     1381 ccccctgctt acgtttttct aacattcctc tgttattttt tttctcgtaa tatcgaacaa
     1441 ggagcaagaa aaaatgccta ccgaatcgca tgaatttaag gctgagattc agcagctgct
     1501 gaatattctg gttcattcgc tctataccga tcgcgatatc ttcctgcgcg aattgatctc
     1561 caacgcgtcc gatgcactta gccgtattca atttgagatg ttgaccaatc gcgaggtgct
     1621 tgatcacgac acggagttgg cgatccacat cgactacgat aaagaagcca agacgttcac
     1681 gattactgat tcgggcgtcg gcatgacgcg tgacgagatc gtcgaaaact tggggaccat
     1741 cgcgcactcc ggggccgccg aattcctgaa acgagttcag gttgataaga aatccgccga
     1801 cgtgatcggt cagttcggcg taggcttcta ttcggtttac atggtggccg atgaagtgac
     1861 agtgacttct cgatcgtatc gccaggatat gcaggccgtg cgctggacat cgaaaggcga
     1921 caatgcctat acgctcgaag aggctgagaa agccgatcga gggacggaga tcgtcgtgaa
     1981 gttgaaagag gatgcccttg agtacgccga tcaatataag attcagcaaa tcgtcaagaa
     2041 gcacagcgac tttgtctcgt ttccgatcta cgtcaaagac aaagtggcaa atcaacaaac
     2101 tgccttgtgg cgcttatcgc cgcacgacgt cacggacgat caatacaacg aattctatcg
     2161 gcagctcacg ctcgattttg aaaagccgct gctgcagatt catatggtca ccgatgtgcc
     2221 ggtggatgtg catgcgttgt tcttcattcc ggccaaacgc gagccgggga tgttcagctt
     2281 gcgccgcgaa gacgggttga aactgtattc caggaaaatt ttgattcgag aatacttcaa
     2341 agagctgctg ccgaattatt ttcgcttcgt gcagggcgtg atcgattcgg aagatttgcc
     2401 gctcagcgtc agccgcgaag cgatccaatc gaatcgcgcg ctggcaaaac tcaaatcgac
     2461 actgacgcac aagatcgccg gcgagttggc tgaactcggc gagcaggatg ccgcccagta
     2521 caatgctttt tggaaagagt ttggcgcgtt catcaaggaa ggcattgcca ccgatgcctc
     2581 gaatcagatc gagttggcga agctgctgcg cttcaagacc aatcgcagcg ccgataaatt
     2641 gacttcgttg cagcagcatg tcgatcgaat gaagcccgat cagaattcga tctattacat
     2701 tttgggcgaa gatttgatgt cgatcgcgag cagcccgcat ctcgattact tcaagcagca
     2761 cgatctggaa gtgctgtatc tcgtggagcc gatcgacagc ttcatgatgc tgaccctcaa
     2821 agaatttgcg ggccgaaagt tgcaaagtgt cgcggatgct aacctggctc ttcccaagtc
     2881 cgatcagccg gtcgaatcga aagtggtgaa tgatgtttat gctcaactcg tggcgcggat
     2941 caaaaatacg ctcggcgaaa aaatcaccga agtgcgcgag tccaaagttt tatccgagag
     3001 cccgtgccgg ctagtctcgc ctgaggagtc caccgatcaa gaaatgcagc gggtgaggcg
     3061 gatgctgggc caagagtacg agattcccaa gaagatcatg gagatcaatc gggggcatca
     3121 attgatcgcg gatttgggct cgctcgctaa gtccggatcg aacgacgcgc tgctcgatgc
     3181 ctgcatccag cagttgtatg agagttcgct gttgatcgaa ggactgctgc ccaatcccgc
     3241 cgaaatgatc ccgcgcattc agacgttgat ggaagcggcg gtaagggaaa agtgatcact
     3301 cgtcacgcat cattcttttg aaaaatcttc tcattttgag tatagtttgc ccgatcgata
     3361 cgaccggatc actttcattc gatgacggta tcacccgccg aaatctatca actggtctta
     3421 gctgcgctac atcgcctaca agatgatggc ggccagtttc aacgcgccat tgtcaggaaa
     3481 cagttatccg acttagagtt ctggctgctc agccgcctgc gttattttcc cgcttcgatc
     3541 acacccggcg atttccaaac ctatgggccg tacacttctg tggcgaacta cgaacaatca
     3601 ctcgaagcgt taaccgccaa gggtttagtc gagaaagtag aaacgaaccg gtatcgatcg
     3661 gcggagaccg gacgcaagtt gatcgagaaa ctctatcacg cttacttcag ccggattgcc
     3721 aaacatgatt ttctgcacgc cgcggaaatt cagcggttga gcgcattagc cgatcaagta
     3781 ttgaactccg cactgcacca gcccaatctt ccgacgccga ttacacaagc gacctttagc
     3841 gttttccctt caaacgaaga tgtgtgggtc atggctgaac ggcgcatggt tgcactcagc
     3901 gcttttcgca gtgacagcca catcgccgcc tggcgcgata aaggctggag cggaccggaa
     3961 gtggcgttaa gcacagcgct ctttcaatca gctgacggac tcgatcacga tcaactgcgc
     4021 gcggcggccg tagccctcaa cgacaaagat ttcaaatcgg ccttatcggc tttgtcggcc
     4081 gacggcgaaa tatcttccaa ctacgatcgc tatcggttgt cggctgccgg acaaacctct
     4141 cgtcagaaaa ttgaagaccg caccaatcgg aactacagcc tgccgttcaa cgcactggaa
     4201 cccgttgagc gggatgagat tgtcgagtta ttagaaaaaa cgcgcgggcc acaatcgaca
     4261 tagatccaga tcgatcccgt atcagatcgc tcctattgac atttgaaatc gggtatgcta
     4321 ccattttaaa cgtaatgcaa gcctgttaat tatttgctgc cagagccggc ggcggttccc
     4381 tgtttattgc ttcagtagtt cgcgcactca tcgttttatc ggcagcactg atcgttgaca
     4441 ggctcagctc tccgatagtt catgattttt gagtgcgccc ttccggtgca ctcattttta
     4501 tttctgaaag gcatcattca tgcgcataga attttttctc cgattgattg gcttgttagt
     4561 ttttggcatg ggcggcttgt tcatcggcca acaacttgtc agcagcggtg gcattgcgtt
     4621 gcaggatggt atcgcttcga ttgcggtgac aacgttggtc ggtggtctga taggtttcat
     4681 tcttatcccg tggtttacca cgcggccctt cagcgcgctg caacggcaaa ttcggcaagc
     4741 accagccgtc gatctggccg cggtcatggc cggcctgggg ttcggtttat ttatcgccgt
     4801 gctcacttca tttccgctgt cctttctgcc gacgccgttc cacgaaatat tgcccgtgat
     4861 tagcgcgatc gtgtttggca cgctcggcat gtggacactg ggcacgcgcc ggcgcgatct
     4921 ggcagcgctg ttcggaaatc ggttgccaac ccgatcgatt gaagctggcc caccggttgg
     4981 cgaagaacgt tcggtgctgc tggatacttc cgtcatcatc gatggtcgca tcgccgatat
     5041 cagtcaaaca ggttttgtta ccggcacgat gctggtgcca cgctttgtgc tgaatgagct
     5101 gcagcacatc gcggactcgt cggatgtcct acggcgcaat cggggccggc ggggactgga
     5161 tatgctgaat cgaatgcaaa aagattcgcg ctcgctgctg cggatcaccg atatggacat
     5221 cgaagaagtc cgtgaagtcg atgacaagtt agtctcttta gccaagcgct tgaagtgtcc
     5281 ggtgttgacc aacgattaca atctcaatcg cgttgccgag attcaaggcg tgacggtgct
     5341 caacgtcaat gaattggcga atgcggtaaa gttggttttt ctacccggcg agaatattcg
     5401 cgtggaagtc attcaagaag gcaaagaagt cggtcaaggt gtaggttatc tggacgatgg
     5461 cacgatggtc gtcgtcgaag aaggccggcg cttcatgggc agcacgctgt ctgtaacggt
     5521 gaccaagatt ttgcagaccg ccgccggccg catgatcttt gcacgaccgg acaatttgaa
     5581 ttagcgcagc tctcacccaa acgctagcgc gcactgacta aaacacattc tgagtgatcc
     5641 gctagattgg tatgcgatct atattgagaa tgcttaaatc atgactattc aagtttataa
     5701 cgtgctgacc catcaaaaag aagaattcaa accgctgaat cccgatctgg tcacgatgta
     5761 tgtctgcggc ccgaccgtct acgatctcag ccacatcggt cacgccaaaa cctacgtcgg
     5821 ctttgacgtg attgtgcgct atctgcgctt cagcggctat cgtgtgcgct acgtgcagaa
     5881 catcaccgat gtcggccatt tactcgacac gggcgaagat cgaattttga agggcgtgcg
     5941 ccgtgagcgc atcgaaccga tggaacttgt cgagcggtat acgcgcgcct attttgcgga
     6001 catggatgcg ttgggcgtcg tccggcccga tatttcgccg cgcgcctctg cgcacattcc
     6061 cgaacagatt gaaatgatca aaaaactgat cgccgacggc tatgcctatg aagtcgctgg
     6121 ctcggtttat ttctccgtcg aaaaatttcc ggaatacgga aagttgtcgg ggcgcaaagt
     6181 cgaagaagca gaagaaggtg cgcgcctcgg agtgcgtgac gagaaacggc accccgccga
     6241 ttttgcgctg tggaagaaag cggagccgga acacatcttg cgctggccca gtccgtgggg
     6301 ctggggtttt cccggctggc acatcgaatg ctcggcgatg tccaccaagt atctgggcga
     6361 gacattcgat attcatggcg gcggcatcga caatattttt ccgcacaatg aatgcgagat
     6421 cgcgcaagct gaagcggtca cccatcagac cttcgcgcgc tattggttgc taaccggatc
     6481 gctgtcgctt gaaggcgtga agatgagcaa gagcctgggt aatacactga cgattcaaga
     6541 tgcgttgaag cgttggcctg cgcaggcaat tcgatatttc atcttgagca gccattactc
     6601 caacccgatc gatttcagcg acgaagcgat cgaagccgcg cacaaaggct tgaatcgagt
     6661 gctggtcccg gtgcgagccg tgcgcgatcg attgcgcacc gcgaccgaca ccgacgaggc
     6721 cgccgacttc aaagcgacgc tcgatcaaca tcgcgcagct ttaaccgcgg ccatggatga
     6781 gaattttaac gcgccggctg cgctcggtat tttgagccag ctgaccaccg atgtcaacac
     6841 actcttgagc agcggccaac cgatcggcca acagacgctc gctgcgatcg atcaactgta
     6901 tcgcgatttt ggcggcaccg tactgggcat catccctgat gaattcaagc cggaatcaaa
     6961 tgcggaattg ctggacggcc tggtgcggat gttaaccgag ctgcgcacag aagcgcgtgc
     7021 aactaaagac ttcaagcgag gcgatcaaat ccgcgatcaa ttggcggagc tcggcgtgac
     7081 actggaagac agccgcgatg gcacaacgtg gcgattgaat aaataatggt taagggcgag
     7141 gttctctcgc ccttatttgt tgatgacaat gaaagaaacc atttacggtc gcaatgcagt
     7201 ttatgaaacc ttgaaagcca atcggcggca agtgtatcag ctgctcgtcg ccgaaggcgt
     7261 tcaagaaaaa ggcaatctgg ccgaagcgct aaagatcgct gcgcaaagat cgatcccggt
     7321 tcagcgcgtt aaacgggaga cgctggatca tcaaaccgac agccatcaag ggttgatggc
     7381 cgaagtcaat tcttatccct atgctggcat tgaagacatc ctcgatctgg cgaagcagcg
     7441 ccatgaactc ccgctgattt tgtttctcga tgtcattcag aacccgcaaa atttggggac
     7501 gctgctgcgc acagccgaag ccattggcgt acatggcgtc gttattccgc agcggcgcgg
     7561 tgtcgaggtg acgcaggcag tcgtcaatgc ctcatccggc gcgagtgaac atctgctcat
     7621 tgcgcaggtc gtcaatctgg tggtgatcat gacggacttg aagcagcagg aaatttggat
     7681 caccggcctt gataatctgc ccgaagccaa ttcgatcgaa gaagcggatt tatccggcgc
     7741 gatcggtttg gtggtcggca atgaaggcga agggatgcgg cggctggtgc gcgaaaactg
     7801 cgacttctta gtgaaactgc cgatgcgcgg tcaaatggaa tcgctgaacg cggccgtcgc
     7861 cggatcgatc gtgttgtatg aaattcttcg gcagagaatg cggtcacgat cgtagacatg
     7921 cctgcccaca tcacgtcggc ctgtgacctt tacgattctt tctctaaaat aatagtcacc
     7981 ggcccatcat tatgaatctc tacctgcatg attgtttgaa agacgccgat ctgaaccggc
     8041 acaccttcag ccctcaatcg atcaacgaag tgtgagaata gcggttcggc tttcgccggc
     8101 gcctccgcat cgatgaatgt tgggcgacgc cctttgcgcg catcagcata tagcgtgaat
     8161 tgcgacacga ctaacgcctc acccttcaaa tccaagatcg atcgattcag cttgccctcg
     8221 tcatcatcga acacgcgcag gttggcaact ttattcgcca accattcggc ttcggcttgg
     8281 gtatcgccga ctttcacgcc ggcaagaata caaaggccac ggccgcactg cccgacgatt
     8341 ttaccatcga cactgacgct ggcacttgag acacgttgaa tgacaagtcg catgatctct
     8401 cctaagagac tgtttcttaa agggtcattc gcctctgtat tgccctgtat tcaatcacac
     8461 ccacgaaggc gggtgtccca ttttctttaa cgcataaagc tggtatcgtt gctctcgggt
     8521 gagctgagtg cacatgctca ggagcgctcc tttgacttgg tcctctaaga gtgctcatcc
     8581 tacctcagct gacctctgtc gacaacctga aaagtcgcac ttgtgagttg attctaatag
     8641 ataaagacgg aagagtgagg gctaaaattg cttgagatgg gacatttgtt acagcgatac
     8701 ctatgtcatg tggtagttga cgcgctgtag gggatatgtt aaaaacacaa tacgccgcac
     8761 acctgacctg gcctaatgcg gctcgatcat tgctcattgc aaaatgtgca ttgaaaattt
     8821 cttcaggagg cagcctctca tgaatatcgg caacattctc gccaccaaag gcagtcgcgt
     8881 catcaccatc caccctgagc aatcgctcaa agaagcgatc gccctactga cccaacacca
     8941 gatcggcgcg ctggtcgtgg tcgataaggc cggcaagccg gttggcattt tgactgagcg
     9001 cgacatcgtc cacgccgccg atcgcagcgg tgaagctatg ttgaaacatg cggtcaaaga
     9061 tgtcatgacc aagaacgtcg tgatcgccac accgcacgac gagctgcaat cggtgctgaa
     9121 gactatgagc gatcggcgct tccggcacat gcccgtgatg gatgaaggcc aactggccgg
     9181 catcatctcg atcggtgacg tggtcaaagc acagctggat gaatatcgcg gcgccatcga
     9241 tacgctgcaa tctcaacttt aaggcaattg ataatgacac tcgttggcac agacaccaat
     9301 ccactgcggg tggcgatcat cggctcaggc cccgcagcct tttatgccgc cgatgaattg
     9361 ctcaagcaaa aagatctcgt gatcgagatc gatatgttcg atcggctgcc gacacccttc
     9421 ggcctggtgc gcggcggcgt tgcgcccgat catcaaaaga tcaaatcggt caccaaagcg
     9481 tatgacggca tcgcctccca tccgcgcttc cgattttacg gcaacgtcga gtacggccag
     9541 cacatcaagt tgatcgatct caaagatcac tatcatcaaa ttttattcgc gacgggcgcg
     9601 cagaccgatc ggcgcatggg catctccggc gaagatttgg acggcagcca tcccgccact
     9661 gaattcgtgg cgtggtacaa cggccatccc gattatcgcc atctcaagtt cgatttgtcg
     9721 cacgagcgcg tggcagtcgt cggtgtcggc aacgtcgcca tcgatgtggc gcgcatcttg
     9781 tgcctcacgc ccgcagagtt ggccgagact gacatggccg attacgcgat cgacgccttg
     9841 agccacagcc aggtcaaaga agttcatctg ctcggtcgac gcggcccggc gcaagccgcc
     9901 ttcaccaatc cggagatcaa agaagttggc gaactcgaag atgcggacgc gatcacattg
     9961 agcgatgaag tgcagctcga tgagttgagc gtgaccgaac tggccagctt gaacgatcgg
    10021 acgctgaata agaaagttga aatcatccag agctatgccc acgctgcgcg ccgatcagtt
    10081 agcaaggccc gaaagcttta cattcgattc ctggtctcac cgacggaaat gatcgacgac
    10141 ggtcacggcc aagtctgcgg cttgaaactc gtgcataacg tgttatacaa aaccgaggcg
    10201 ggcacgctca gcccgaaagc gaccgatcaa tttgagcagc tccatgtcgg gctggtgttt
    10261 cgatcggtgg gctatcgcgg tgtgccgctg cccgaagtac cgttcaacga tcgctatggc
    10321 gtgatcatga acgacaaggg ccgcgtgatc gaagtcgagt ccaagcagca ggtgattggc
    10381 gaatacgctg ccggctggat caagcgcgga ccgagcggcg tcatcggcac gaataaaccg
    10441 gactcggttg aaacggtgac gcacatgttg gaagacttga ggagtggatc gatcttgcag
    10501 ccggtgcagc ccacagtgaa cagcatggcc gatttaatca aacagcgaca gccgcaattc
    10561 ttctcgtatc ttgattggaa gaagcttgat gaactggaag tggcgaaggg tcagccgctg
    10621 ggtcggccgc gcgtgaaatt caccagcgtc gcggaaatga tcacggcgtt gggcaagtag
    10681 attgaaaccc gaaaggtttt gaaaaccttt cgggtttttt aaccatgaaa ctcgttactg
    10741 tcgatcaaat gcgcaagatc gaaaccgccg ccgatgcgag cggacattct ttcgcgcaga
    10801 tgatggaact ggctggcagc gccgttgcgc gtgtgatcca agatcggttg gacgtgcgcg
    10861 gcaaaagaat tttgctgctc atcggcccgg gcaacaacgg cggcgatggg cttgtcgcgg
    10921 cacgggtgtt gaaagatgcg ggcgcggaca tcggcgtgta tttattgaaa gcgcgcgacg
    10981 atgaattgat caagacgttg cgttcacgca gcgtcttcat cgccgcgctg cccgacgatc
    11041 gcggtctgcg ctttttgcat ttgtggttaa gcaactgcga tctcatcatc gatgccttac
    11101 ttggaacggg cagcgctcgg ccgatcagcg gcgatttggc taagttactg aatgcagtca
    11161 acgccgaagt cgctactcga cgggcggcat acacctcaac gcttcgttca ctttcatggc
    11221 ccggcctcga aacgcttgcg cccttgatcg tcgcgatcga tggtccaacc ggtttgaatt
    11281 gcgacacagg cgaacttgat cccaactcgt taagcgccaa tatctccgtc acatttgctt
    11341 atccgaagat cggacatgcg cgcttgcccg gcgcgcgcgc cgtcggcgag ttgatcgtcg
    11401 ccgacatcgg gatcgatccg caacttgcca aagatatcga acttgaactt gcggaggcgc
    11461 agttgattcg atcgttattg cctgcgcggc cgatcgacgc acacaaaggc acattcggta
    11521 aagctttaat cgtcgccgga tcgatcgagt ataccggcgc gccgatcttg tccgcgaccg
    11581 ccgcgtatcg atccggcgca ggcttggtca ccctcgccac gccgcgatcg atccacgcca
    11641 tcatatctaa caagatcaat gaggccacgt ttctgccact gcccgatcaa aatggcgcgt
    11701 tatccgccga agccgttgag ccgttgatcg aaatgctgaa aacgtatgac gcagttttga
    11761 tcggaccggg tttgtcattg aaagcccagt cttttattga ttccgtgttt aattcaacat
    11821 tatggccgcc gctcgtggtg gatgctgatg cactgactat tctggcgcag atcgatcgat
    11881 ggtggacgcg tgtgcccgcg cctgcaatct taacgccgca tcccggtgag atgtcgcggc
    11941 taacgaagat cgatcgggat cagatcgaag ccgatcggtt aggcatcgcg cgcaagttcg
    12001 cggcggtgtg gggacaggtg gtggtgttga aaggtgcctt cagtgtgatc gccgcgccgg
    12061 atggtcgatc gatcatcatg ccgtttgcga atccggcctt agccaccgcg ggcagcggtg
    12121 atgtattggc cggagtgatc gtatcgatgc gcgcccaagg tctagctgct tatgaagccg
    12181 cgatcgtcgg aacatacttg catggactgg caggcgagat cgcccgtcgg gagataggct
    12241 taagcggagt tctagccggt gatttgctgc tgaggttacc tcaagcgatc aaacagtgca
    12301 gcgcggtata gctgttacgc ttacgacttt ggagtgttgt ccgggtgtcc agcgtcctcg
    12361 cccgtcaacc ccttgcgaaa ttcacgaatg cctttgccca gatcgctgcc caaatgcgcg
    12421 atgcgtccgc cgccaaacaa caacaagaca attgccaaaa taatcaacag ctcagtcggt
    12481 cctaaatcca tgccctaata cttttagcca gatttatcaa cgaccacagt ttaacccaat
    12541 ccgtcaagcc gtgccttaaa cgcggttaaa tattgagtga gaaaaataaa actctggcga
    12601 cactgactcg ccagagtttt gttttaaatt gaat
//
LOCUS       DASRTJ010000702         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_371918,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000702 DASRTJ010000000
VERSION     DASRTJ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371918"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFF70_14200"
     CDS             complement(<1..1069)
                     /locus_tag="VFF70_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZY45887.1"
                     /translation="MITQPLSPRQFATLIAICDTLVPSIERDDDPSGFWKRKASDLNV
                     PQLIVETVFTLQDEVSQAQFKQLLSLLDQPITSLALTGHFKRFVDLSLREREKTLQRW
                     SLSSIGLLRQGFQGLKRLTHVLYYITLDANGSNPNWQVMGYPLAPQLQRQRSSAALRS
                     TTVDSDLTLECDAVIVGSGAGGGVVAGELAQAGKAVIVLEKGGHFDENTFPDKEAEAY
                     RLMYDNQGILTTRDYGVVLLAGSTLGGGTTVNWAASFRTPDFVLEEWSRAYGLKGFLD
                     SSFQQSLDAVCQRSSVNVAEAKPNPQNQKLQQGCLELNYHAAPIPCNVQSCGDTRQCG
                     FCGFGCALGSKQSTLKTYLQDA"
     gene            complement(1288..1854)
                     /locus_tag="VFF70_14205"
     CDS             complement(1288..1854)
                     /locus_tag="VFF70_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45888.1"
                     /translation="MTNNKIFFKQHLLRAALLISIGIILIACSNAQTPPNTAQAIEYR
                     GAQFGFRLSYPAQWQVLEDPPPMVGDNPTILHAVTLQPPQDSKALVVVYIQTLTTTQT
                     LAEYASQQMISLGANEVGATFSDLAVTQLGGLDARSTNAADDKSQLRRMIITLNNAKA
                     YAVLLFGPPSNDLAAQFETMVQSFRFLP"
     gene            complement(1854..2606)
                     /locus_tag="VFF70_14210"
     CDS             complement(1854..2606)
                     /locus_tag="VFF70_14210"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY45889.1"
                     /translation="MEFKEKVVLVTGAGRGIGKAIALAFAQAGARVAVNDINPDSCEV
                     TANEINAIGGEATAWHCDVTNKLGVQTMLIGIEDHWQQIDILINNAGVEPHKSIVTMD
                     EWDWDRTIDVNLKGAFLCSQSVGRMMRDRDGGAIVNIASIAGRAAGLRDRSAYVAGKA
                     GLIGFTKECAREFAPYHIRVNAVCPGVIITEMTAPLQQNAEQMKKWLEDIPLGRLGDP
                     ADVTGLVLFLCSDAARYITGQAINVDGGKVML"
     gene            complement(2664..3632)
                     /locus_tag="VFF70_14215"
     CDS             complement(2664..3632)
                     /locus_tag="VFF70_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZY45890.1"
                     /translation="MTDSRPYVGQPADSFNFKNILYRKQNRVATVTFNRPELLNCINY
                     ATLLELSTAFHDAAFDDAIAVVVLTGAGDRAFCTGADMKEQQQFIERPRDYYKWMGAF
                     IEAHEQLRNLGKPTLARLNGIVVGGGNEFNLSCDLAIAADDIYIRQVGASHGSVAAAG
                     ATQWLPLIVGDRRAREILMLNEEIPAQKGLEWGLVNQVVPRSELDNAVAMMAQKLIDK
                     FPEIMRYTKQQLNFWREFSWHMTIGHARDWLSIHTTSPETLEGVQSFAEKRTPNYEGL
                     RDRAEQDRSPEYPHGAPSINCRHCGMQNLPSQFKYCGNCGKTLDKQ"
     gene            complement(3664..3984)
                     /locus_tag="VFF70_14220"
     CDS             complement(3664..3984)
                     /locus_tag="VFF70_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZY45891.1"
                     /translation="MVKLIALYKKPADVEAFEQHYTQIHIPLIEKIPGIRKTEWSRIT
                     ASPTGEAPYYMIYEMYFDNMDAYKVAMKSEENKAAGKDLMSFAKEVVTLMVADAYEDE
                     IKIK"
     gene            complement(4115..>4823)
                     /locus_tag="VFF70_14225"
     CDS             complement(4115..>4823)
                     /locus_tag="VFF70_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45892.1"
                     /translation="PFFISKNDLSIINSTGLTLILFNEDDAPSGEGASIIGRATEVRI
                     AEGDDTSNAAWQPWAASIDFQLSDTPGSHTITIEYRDDQQRSTKYSRTIILGLVDNTE
                     PTVTPIATLAPTSAPTLEATIVVINTLTPTETATPLPTETLPPTDTPEPTSTSTLEPT
                     STAKPSPTSTPTPSITPTPPFGSRLTPPAPVPVPTDRPLDGSLAGMPDWLLPAFGLLQ
                     VLVLIVGAVVVVKRSIK"
BASE COUNT         1091 a         1350 c         1256 g         1126 t
ORIGIN      
        1 cggcatcctg caaataagtc ttgagagtgc tctgctttga acccagcgcg cagccgaatc
       61 cgcagaaacc gcactgccgg gtgtcgccgc agctttgtac gttgcacgga ataggcgcag
      121 cgtgataatt caactcgaga cagccttgct gtaatttttg attctgtgga ttgggctttg
      181 cttcggcgac attcacgctg gagcgttgac acaccgcatc cagcgattgc tgaaagcttg
      241 aatcgagaaa acccttaagg ccgtaagcgc gtgaccattc ttctaaaaca aaatccggcg
      301 tacgaaatga ggccgcccaa ttcaccgtgg tgccgccgcc caacgtcgat ccggccaaca
      361 agacgacgcc ataatcgcgc gtggtcaata tgccttgatt atcatacatc aagcgataag
      421 cctccgcctc tttgtctgga aaagtattct catcgaagtg gccgcctttt tccaaaacga
      481 tcaccgcctt gccggcttgg gctaactcac ctgcaaccac tccgccgccc gcacccgatc
      541 cgacgatgac cgcatcacac tcaagcgtga gatcagaatc aacggttgtc gaacgcaagg
      601 cggccgagga acgctgccgc tggagctgcg gcgctagggg atagcccatc acttgccaat
      661 ttggattgct gccattcgca tccaaagtaa tgtagtacaa aacatgcgtc aagcgcttga
      721 ggccttgaaa gccctggcgc agtaaaccga tcgaggacag tgaccagcgc tgcaaagttt
      781 tttcgcgctc gcgcagcgac agatcgacaa agcgtttgaa atgaccggtc aacgccaggc
      841 tggtgatcgg ctgatcgagc aggctcaaga gctgcttgaa ttgcgcttga ctcacttcat
      901 cttgcagcgt gaacacggtc tccacaatca gttgaggcac attcagatca gaagccttgc
      961 gcttccagaa accggacgga tcgtcatcac gctcaatcga cggcacaagt gtgtcgcaga
     1021 tggcgataag tgtagcgaac tgtcgaggag aaagaggttg agtaatcatg tagacaagcg
     1081 ctcatcgcgc gtgttgtgtt gcgtcaggaa aatgatcgcc gtgttattcg tgccgggcat
     1141 ctcacgcaga gcttgattca cctcatagcc gccgcttcag tgcgatagcc cagcgtattg
     1201 aaatagaatc gcatcaatcc acggagatca ccatcctcat cgacgatcaa tagatcgacg
     1261 gttgagtcaa gtgtggagct aaattgatca tggcagaaac cggaacgact gcaccatcgt
     1321 ctcaaactga gcggccagat cgttactcgg cggaccgaac agcagcaccg cgtaagcttt
     1381 agcgttatta agcgtgatga tcatccggcg caattgcgat ttatcatcag ccgcattcgt
     1441 cgaacgcgcg tccagcccgc ccaactgggt gacggccaaa tcagagaacg tggcgcccac
     1501 ttcattcgcg cccaggctga tcatctgctg actggcatat tcagccaatg tctgggtcgt
     1561 cgtcagtgtt tgaatgtaaa ccaccaccag ggctttggaa tcctgcggcg gttgcagcgt
     1621 gaccgcgtgc aagatcgtcg gattatcgcc gaccattggc ggcggatctt ccagcacttg
     1681 ccattgagcc gggtaagaca atcggaaacc gaactgcgcg cctcggtatt cgatcgcttg
     1741 agcggtattg ggcggcgtct gcgcgttgct gcacgcgatc aaaattattc caatcgagat
     1801 taataaggca gcgcgcagta agtgttgctt aaaaaatatt ttattgtttg tcattaaagc
     1861 attactttcc caccatcgac gttgatggct tgcccggtga tatagcgcgc ggcgtcggag
     1921 cataaaaaca aaaccagacc ggtgacatct gccggatcgc ccaaccggcc aagcggaata
     1981 tcttccagcc atttcttcat ctgctctgcg ttctgctgca agggcgccgt catctcagtg
     2041 ataatcacgc cggggcagac cgcattcacg cgaatatgat acggcgcaaa ttcacgcgca
     2101 cattctttgg tgaagccgat caaacccgcc ttgcccgcca cgtaagccga tcgatcacgc
     2161 aagcccgccg ctcggccagc gatcgaagcg atgttgacga tcgcgccgcc atcccgatcg
     2221 cgcatcatgc gcccgacaga ttgcgaacac agaaacgcgc ccttcaagtt cacatcgatc
     2281 gtgcgatccc aatcccattc atccatcgtc acgatcgatt tatgcggctc gacgcccgca
     2341 ttgttgatca agatatcgat ctgctgccag tgatcttcga tgccgatcaa catggtctgc
     2401 acacccagct tgttggtcac atcgcagtgc caggccgtgg cctcaccgcc gatcgcgtta
     2461 atctcgttcg cggtcacttc gcacgaatcg ggattgatgt cgttgacggc cacgcgtgca
     2521 ccggcttgag cgaacgccag cgcgatcgct ttgccgatgc ctcgaccggc gcccgtgact
     2581 aaaacaactt tttctttaaa ttccacattt acctctaacg tgtcactctg agccgcgtag
     2641 cggcgaagaa actgtcttac actttattgc ttgtccaaag tttttccgca gttgccacag
     2701 tatttgaact gcgatggcag attctgcatc ccgcaatggc gacaattgat cgatggcgcg
     2761 ccgtgcggat actccggcga tcgatcctgc tcggcccgat cgcgcagccc ttcataattg
     2821 ggtgtgcgct tctctgcgaa cgactgcaca ccctccaacg tttcgggcga cgttgtgtgg
     2881 atcgacagcc agtcgcgcgc gtgaccgatc gtcatgtgcc aggagaattc gcgccagaaa
     2941 ttcaactgct gtttggtata acgcatgatc tctggaaact tatcgatcaa tttctgcgcc
     3001 atcattgcca ctgcgttatc caattcagat cgcggcacga cctgattgac caacccccat
     3061 tccagcccct tctgcgccgg aatctcttca ttcagcatca aaatttcgcg cgcgcgtcga
     3121 tcgccgacga tcaatggcag ccactgtgtg gcacctgccg ccgcgaccga gccgtgactc
     3181 gcgccgactt gtcgaatgta aatatcatcc gccgcgatcg ccagatcgca cgacaggttg
     3241 aattcattgc caccgccgac gacgatgcca ttcaagcgcg caagcgtggg cttgccaaga
     3301 ttgcgcagct gctcatgggc ttcgatgaac gcgcccatcc atttgtagta atcgcgcggc
     3361 ctttcaataa attgctgctg ctctttcatg tccgcgccag tgcagaatgc ccgatcgccc
     3421 gcgccggtca gcacgacgac cgcgatggcg tcatcgaacg cagcatcatg aaacgcggtt
     3481 gagagttcaa gtaacgtggc atagttgatg cagttcagca attccggccg attgaaagta
     3541 accgtcgcca cgcggttttg tttgcgatac agaatatttt tgaagttaaa cgaatcggcg
     3601 ggctggccga cgtacggtcg agaatcagtc attcgtcatt cgccatttat ctttggtcaa
     3661 ttgtcatttg attttaattt catcttcgta cgcatcggcc accatcagcg taaccacctc
     3721 tttggcaaag ctcatcaaat ctttgccagc cgctttgttc tcttcggact tcatagccac
     3781 tttataagca tccatgttat cgaagtacat ctcgtagatc atgtaatacg gcgcttcacc
     3841 cgttggcgaa gcggtgatgc ggctccactc ggtcttacgg ataccgggga ttttctcgat
     3901 gagcgggatg tgaatctggg tgtaatgctg ctcaaacgct tcaacatcgg cgggtttctt
     3961 gtagagggct atgagtttga ccatgaagaa aatctcctaa atgaactggc ttcgattata
     4021 acactgctgc cgatcattca gcgagttgga tcgttaattg atctcgatcg agtttggcgc
     4081 ttgaacaaca agggtgttac cccttattca tttatcattt gatcgatcgc ttgaccacca
     4141 caaccgcgcc gacgatcaat accaggactt gcaacaaacc gaacgcgggc aacaaccaat
     4201 cgggcatacc cgctaaactt ccatccagcg gtcgatcagt tggcacgggc acgggtgcag
     4261 gcggggtgag tctcgatcca aacggcggcg tcggcgtgat cgatggtgtc ggcgtcgaag
     4321 tgggtgacgg cttcgctgta gatgtcggtt caagtgtaga ggtggatgtc ggttcaggtg
     4381 tgtctgtcgg cgggagcgtt tccgttggca gcggcgtggc cgtttccgta ggtgtcagtg
     4441 tgttgatgac aacaatcgtc gcttcaagcg ttggcgcact cgtcggggca agcgtagcga
     4501 tcggggtgac cgttggctca gtgttatcca ccaaacccaa tatgatggtg cgggaatact
     4561 ttgtgctgcg ctgctgatcg tcacggtatt cgatcgtgat cgtatgcgag cccggcgtgt
     4621 cagacaattg aaaatcgatc gacgcggccc acggctgcca agctgcattg ctcgtatcat
     4681 ccccttcggc gatacggact tcagtggcac gcccgatgat gcttgcgcct tcgcctgatg
     4741 gcgcatcgtc ttcgttgaac agaattaaag taaggccagt ggaattgatt atgctcagat
     4801 cattcttgct gataaagaac ggc
//
LOCUS       DASRTJ010000703         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_297714,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000703 DASRTJ010000000
VERSION     DASRTJ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297714"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1019)
                     /gene="paaA"
                     /locus_tag="VFF70_14230"
     CDS             complement(42..1019)
                     /gene="paaA"
                     /locus_tag="VFF70_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HZY45893.1"
                     /translation="MSVLSEAEIKIDTNEPQSGPEFESKLAIFEARIDRGDKIEPSDW
                     MPLDYRKQLIRMISQHAHSEIVGCLPEGGWITNAPTFRRKLALTAKVQDEAGHGQLLY
                     RAAETLGITREEMIDALLTGKAKYANIFNYPAVTWADVGMIGWLVDAAAIVNQLMLAE
                     GSYGPYARAMKRICYEESFHLKQGQHFIVTLAGGTRQQREMAQAALNRWWWPTLQMFG
                     PNDKDSVHLIQMMRWKIKTKTNDELRYAFIEQYTPQIQRVGLVIPDPELRFDEKQNRW
                     LYGEPDWEEFKRVISGHGPMNAERLAARRAAHEDGRWVREAMAAKAMNN"
     gene            complement(1153..2868)
                     /locus_tag="VFF70_14235"
     CDS             complement(1153..2868)
                     /locus_tag="VFF70_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZY45894.1"
                     /translation="MNKPHTLYSVKVPMRDGIHCAANVYFPEGAGPWPVILNRTPYIK
                     DSALRAKRVEPYVNAGYVFVSMDVRGRGDSEGEYNPNFQEIEDGYDAIEWCGTQSWST
                     GKVGTQGGSYEGWTQVYPMRLRNKHHTAAFLQCTPSMHPFHEGFYWSGVPQPIMGMMA
                     FYTSGHSIKEQIVNFDWADTLDIRPLKDLHAAIGLPASLRDEQFKHQTFDEHYQKLWF
                     EGVSDLINVPCYHITGWFDDDQKGTLELFPALALNHPDPNVRRSQKLLIGPWPHRMSS
                     DSSKLGDFDYGPHSMVPLAKEAIRWFDYWLKGIDNGIVEEPRCRMFLMGENRWIESDT
                     FPIDQARGKIFYLAADGPSNSLLGHGKLIDKPISITLSDTFTYNPARPAPTPFWKENF
                     QNGTNEDLRSIQRRDDVLVFTTEPFKEPLNIVGILKAELYVSTSALDTDFVARLSDVQ
                     PDGYAMRLNAGILRLRYREGYDQMKLVKPGEIVKIEIDLWATGYQFQAGHCVRLEITS
                     SAYPTWAPNYNTGGNVWEETEPIVAQQTVYHSAQYPSMLILPEMPNPKIVDAWPESRW
                     SRDEG"
BASE COUNT          642 a          834 c          794 g          788 t
ORIGIN      
        1 tcattaagca ttgatcattg tgcattgcac attgcccatt gctaattgtt cattgccttc
       61 gccgccatcg cctctcttac ccagcgaccg tcttcgtgcg ccgcgcggcg agcggctaag
      121 cgctcagcat tcatggggcc atgtcccgaa atcacacgct tgaactcttc ccaatcgggt
      181 tcaccgtaca accagcgatt ttgtttttca tcgaaccgca attccggatc ggggattacc
      241 aaacccacgc gttgaatctg tggcgtatat tgttcgatga aggcatagcg cagttcatcg
      301 ttggtcttgg ttttgatctt ccagcgcatc atttgaatga gatgtaccga gtctttatcg
      361 ttcggaccga acatttgcag tgtcggccac caccagcgat tgagcgcggc ttgcgccatc
      421 tcgcgctgct gcctggtgcc gcccgccagc gtgacaatga aatgctgtcc ctgcttcaag
      481 tgaaatgact cttcatagca aatccgtttc attgcgcgcg cgtatgggcc gtatgaacct
      541 tcagctaaca tcaactgatt gacaatcgcg gccgcatcga ccaaccaacc gatcatgccg
      601 acatccgccc acgtcacggc cggatagttg aagatgttcg cgtactttgc tttgccggtc
      661 aacaacgcat cgatcatttc ttcgcgcgtg atgccgagcg tttctgcggc gcgatagagc
      721 aattggccgt gaccggcttc gtcctgaact tttgcggtca aggcgagttt gcgccggaag
      781 gtgggcgcat tcgtaatcca gccgccttcc ggcaagcagc cgacgatctc gctgtgcgcg
      841 tgctgggaga tcatgcggat caattgtttc cgatagtcca acggcatcca atcgctgggc
      901 tcgattttgt caccgcgatc gatccgcgct tcaaagatcg ccaacttcga ttcaaactcc
      961 gggccgcttt gcggttcgtt ggtatcgatc ttgatttcag cttccgataa aacactcatg
     1021 atactcatgg ctgtgctccg attgacttct catgtttaac cgaatttatt gtaccatgat
     1081 cgtatatcgc ataacgctta tcgcggttcg ctgattcgct tgtcacctgc atcttgcagc
     1141 ctgcgacttg cgtcaacctt catcccttga ccatctactc tccggccagg catcgacgat
     1201 ttttggattg ggcatttcag gcaagatcaa cattgatgga tattgcgccg agtgataaac
     1261 cgtttgctgc gcgacgatcg gttcggtttc ctcccagaca ttgccgcccg tgttgtagtt
     1321 cggtgcccac gtcggatacg cgctggatgt aatttctaat cggacgcaat gtcccgcctg
     1381 gaattgatag ccggttgccc acagatcgat ttcgatttta acgatctcgc ctggcttgac
     1441 cagtttcatt tgatcgtagc cttcgcgata gcgcaagcgc aggatacctg cattcagtcg
     1501 cattgcatag ccgtcgggct gaacatcgga taagcgcgcg acaaaatcgg tatcgagggc
     1561 ggaggtgctg acatacagtt ccgctttcaa gatgccgacg atgttcaatg gttctttgaa
     1621 cggttccgtc gtaaacacaa gaacgtcatc gcgccgctgg atcgatcgca ggtcttcgtt
     1681 cgtgccgttc tgaaaatttt ctttccaaaa gggcgttggg gcagggcgcg ccggattgta
     1741 agtaaaagtg tcgcttagtg taatggatat tggtttatcg atcaacttcc cgtggcctag
     1801 caaactattc gacggtccat cggcggcgag gtagaagatc tttccgcgag cctgatcgat
     1861 cgggaaggtg tcggattcaa tccaccgatt ctcgcccatc aagaacattc gacagcgcgg
     1921 ttcttcgacg atgccattat cgataccttt aagccaataa tcgaaccagc gaatcgcttc
     1981 tttcgccaac ggcaccatcg agtgcgggcc ataatcgaag tcgccgagtt tgctggagtc
     2041 cgaggacatt cggtgcggcc acgggccgat gagcagcttt tgcgatcggc gcacatttgg
     2101 atcgggatga tttagcgcga gcgcaggaaa taattcgagc gtgccttttt gatcatcgtc
     2161 gaaccagccg gtgatgtgat agcacggcac gttaatcaga tcgctgacgc cctcaaacca
     2221 caatttctga tagtgctcat caaaagtttg gtgcttgaat tgttcgtcgc gcagactggc
     2281 aggcaagccg atcgccgcgt gtaaatcttt cagcggtcga atgtcgagcg tgtcagccca
     2341 atcgaaattg acgatctgct ctttaatcga atgtccggat gtgtagaagg ccatcatgcc
     2401 catgatcggc tgcggcacac ctgaccaata gaagccttca tgaaacggat gcatcgacgg
     2461 cgtgcattga agaaatgccg cagtgtgatg tttgttgcgc agtcgcatcg gatagacttg
     2521 tgtccaacct tcatatgatc caccctgcgt gccgacttta cccgtcgacc atgattgcgt
     2581 gccgcaccat tcgatcgcat catagccgtc ttcgatctct tggaagtttg gattgtattc
     2641 gccttcggaa tcgccgcgac cgcgcacgtc catgctgaca aaaacataac ccgcattgac
     2701 atacggctcg acgcgtttcg cgcgcagcgc gctgtccttg atgtacggcg tgcgattcaa
     2761 gatcaccggc cacgggccag caccttcggg gaaataaaca ttcgcggcgc aatgaatccc
     2821 atcgcgcatg ggaactttga cgctgtaaag agtgtgcggc ttgttcaatg ttggctcctt
     2881 gttgttgacg gaagacggcg gtcttctgtc gtcggtctct tagaaatgtc ctctcgccgc
     2941 cgaaatcaaa tcggctaaaa cgccgaacgc cgtttgtgcg ggcgtcggcg cggtttccat
     3001 cagtgtgagt tgattcagcg tatcggtctt gatctggatc gcgctggtcg tgccatac
//
LOCUS       DASRTJ010000704         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_297803,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000704 DASRTJ010000000
VERSION     DASRTJ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297803"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFF70_14240"
     CDS             <1..295
                     /locus_tag="VFF70_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZY45895.1"
                     /translation="KEYQTMPVMCPTVVDAPVAHRIRHVANRAFHALGCRDYARVDMR
                     LRDGVPYVLEVNPNPCLAPDGGFSNAARVAGYDHPAMTKQIADWAWWRRGKHA"
     gene            288..776
                     /locus_tag="VFF70_14245"
     CDS             288..776
                     /locus_tag="VFF70_14245"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY45896.1"
                     /translation="MLELKTEIAPTRPDEAEDILKITADVGVFNDTEVETVRELLAGY
                     YASPIESGYYFLSYRERDRVLGYVCWGPRELSGGAYDLYWICVSREAQGKGAGGALMQ
                     RFEQEARQRGGTWLIIETSSTPHYASARRLYERSGYEKSMELADFYHPGDNLLVYTRR
                     LK"
     gene            complement(889..1575)
                     /locus_tag="VFF70_14250"
     CDS             complement(889..1575)
                     /locus_tag="VFF70_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45897.1"
                     /translation="MKKILIVDDDPDLFKMLGPFLRETGYAIFTATTVLEGLRTVFNE
                     RPDLILLDIMLPGMDGLAMATRVREMSDLPIIVLSAKGTEVDKLRGFNLGIDDYVVKP
                     FSLLELAARIDAVLHRAAKTLQAGSKQQFAFGDLLIDFNRRQVLRGDQPIDLTPTEFR
                     LLQCLAENPNTAVTEARLRAAVWGGAENKDSGYVRRYIWFLRQKLELDPRHPRYLKTV
                     RNYGYRLDVA"
BASE COUNT          394 a          467 c          485 g          383 t
ORIGIN      
        1 aaaggaatat caaacgatgc cggtcatgtg tccgaccgtc gtcgacgcgc cggtggccca
       61 tcgcattcgc catgtcgcca atcgtgcctt tcatgcgttg ggctgccggg attatgcccg
      121 tgtcgatatg cgtctgcgcg atggcgttcc gtacgttttg gaagtgaatc cgaacccgtg
      181 cctcgcgccg gatggcggct tttccaatgc ggcgcgcgtc gccggttacg atcatccggc
      241 tatgaccaaa caaattgccg attgggcctg gtggaggcgc ggcaaacatg cttgagctaa
      301 aaactgaaat cgcgcccacc cgccccgacg aagccgaaga tattctgaaa ataacggctg
      361 acgtcggggt ttttaatgat accgaagtgg aaaccgtgcg cgagctgttg gccggttatt
      421 acgctagccc gatcgagagc ggctattatt ttttgagtta tcgcgaacgc gatcgcgtgt
      481 tgggttatgt gtgttgggga ccgcgcgaac tatcgggggg tgcctatgat ctgtattgga
      541 tctgtgtctc gcgtgaagcg cagggtaaag gtgcgggcgg tgcattgatg cagcgcttcg
      601 aacaagaagc ccggcagcgc ggcggcactt ggctgatcat tgaaacttcc agcacaccgc
      661 actatgcgtc tgcgcgccga ttgtacgaac gcagcggcta tgaaaagtcg atggagttgg
      721 ctgacttcta tcatcccggc gacaacttac tggtgtatac gcggcgattg aagtgatgga
      781 aaaaagcggt gagcccgcaa aagctgatcg ggtagagctg ctcagcgaac ggcggcgatc
      841 aagatcaaaa aaatccggca cagtcgatcg actggccgga tttttgtttt aagccacatc
      901 caatcgataa ccgtaatttc ttacagtctt gaggtagcgc ggatgacgcg ggtccagttc
      961 caatttctgg cgcaggaacc aaatgtagcg ccgcacgtag ccgctgtctt tgttctccgc
     1021 accgccccaa actgctgcgc gcagccgcgc ttcagtgacg gccgtattgg gattctcggc
     1081 caggcactgc agcaagcgaa actccgtcgg cgttagatcg atcggctgat cgccgcgcag
     1141 cacctgccgc cgattaaaat cgatcaatag atcgccgaaa gcaaattgct gtttggaacc
     1201 ggcttgcagt gttttggcgg cgcgatgcag caccgcgtcg atgcgcgcgg ccaattccag
     1261 caggctgaac ggcttaacga cgtagtcgtc aatgcccaga ttgaagccgc gcaatttatc
     1321 gacttcagtc cctttagctg acaaaacaat aatgggcaaa tcagacatct cgcgtacacg
     1381 cgtcgccata gccaagccat ccatacccgg cagcataatg tccagcaaga tcagatcagg
     1441 ccgttcattg aataccgtgc gcaggccttc cagcaccgtt gtggcggtga agatggcata
     1501 acccgtttcg cgcaaaaacg gccccaacat tttgaataga tcagggtcat catccacgat
     1561 caaaatcttc ttcatgtcct gcgtgactcc agtccttgag ggcaaatgtg gacgtcgatt
     1621 cgttcgtaag gggattatag cctaaattgc gtgtgtcgcc taaactcgaa gatcatcgcc
     1681 gcccgatcgc ataactaaat ttagccagcc agcgtcgcag cgatcgctt
//
LOCUS       DASRTJ010000705        11660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_297805,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000705 DASRTJ010000000
VERSION     DASRTJ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297805"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="VFF70_14255"
     CDS             complement(<1..2520)
                     /locus_tag="VFF70_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HZY45898.1"
                     /translation="MMSKSKQAAPTTTLEADDRAVKLTDNARVVLMKRYVRRGPGGKP
                     IESPEGMFHRVAQAVAEPDQEFGLDVEATERAFYRLLTDLRFFPNSPTFTGAGTPLGQ
                     LAACFVLPITDDMGRDSAGIFQTLRDAALIQQTGGGNGFSFGRLRHKGSYVASSAGQA
                     TGPVGFLRVYDKAFGEIAQGGCLLPNTLVFTDRGLLRLDEIVDSNKTGWQDHRLNVAT
                     DQGWRSSPRSFNNGVAPVLRVHTRNGLSLAGTYQHRVKVMTDDGPQWRELQELKSGDW
                     LLVQLGQQRGKLQALRRPKFEHGNQILPKLPSVLDEEFAFFLGYLAGDGFVASAEDDH
                     RVGVSVAHDSYLIEEMPAMLERLFGVKIHRQQKSHDRSVTLVIDNRALKEFLQMNGLN
                     KTRSRDVSVPRLIRQSPSEIVGAYLRGLFEADGGLSHGYPDLTTTSSQLAEEVAALLI
                     GLGCPLRLHSIAPGVNHYGQANIYHVRIESSVGLQAWREKIGCDQRSRFVAAYAWQTG
                     LQRESVYPLPHARYWIEPVLEAITLEQIDAKGRGMGLNFRSDNPRLRRSLLRYSRNDR
                     QLTRSGYDELRRQYPEFAEHARSGDGLWFVQVSGVEPIGESLTLDLEVEENHTYLAYG
                     LVTHNTRRGANMAVLPVHHPDILDFINCKVSEDQITNFNISVGITDDFMRAVRDDQNF
                     DLISPHDGSVTKTLRAREIFNAIVKNAYRNGEPGVLFLDAANRTNPVPHLYALEATNP
                     CGEQWLGPYENCCLGSINLAQHMRTVDGMPQVDWEKLKESTILSTHFLDNVVTANKYV
                     PAVPQLKDAAYLARRIGLGIMGLGDMMYHFGIRYGSAEGQEF"
     gene            complement(2612..3064)
                     /gene="nrdR"
                     /locus_tag="VFF70_14260"
     CDS             complement(2612..3064)
                     /gene="nrdR"
                     /locus_tag="VFF70_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZY45899.1"
                     /translation="MKCPYCESDDTRVIDTAHETTGPGIRRRRECNNCGKRFSTVERA
                     ILTTPLVVKSDGRREPFDRDKLLSGIRMACAKRPISSDALESLVNSIEAKIQQTGKAE
                     VPGKMIGDLVIGGLKPLDQVAYIRFATVYLGLDDVESIRREIDKILER"
     gene            complement(3604..4737)
                     /gene="ftsZ"
                     /locus_tag="VFF70_14265"
     CDS             complement(3604..4737)
                     /gene="ftsZ"
                     /locus_tag="VFF70_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZY45900.1"
                     /translation="MQSNVSSNAEQYARIKVVGVGGGGTNAVNRMIGEGLPGVDFIAV
                     NTDAQALKMTTAPTKVRIGDKLTRGLGAGGNPEVGAKAAEESADDLQAAMSGADMVFV
                     TCGMGGGTGTGASAIVARIAREIGALTIGVVTRPFTFEGHKRSAAAENGINALKEQVD
                     TLIAIPNDRLLEIVDKKATLQQSFRVADDVLRQGIQGISELITVPGLINLDFADVRTI
                     MAEGGAALMAVGSAEGEDRAIKAAQQAITSKLLDVTIDGARGILFNVTGGPNMTLYEV
                     NQAAAIIKETAHPDVNLIFGAVIDDKMGDAIRVTVIATGFDQAGPTPVRRRVSVSAQP
                     SMLTVGSKPMATTDQSLGIPVDFPVRQFDKDDLEVPAFLRKRR"
     gene            complement(4814..6055)
                     /gene="ftsA"
                     /locus_tag="VFF70_14270"
     CDS             complement(4814..6055)
                     /gene="ftsA"
                     /locus_tag="VFF70_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZY45901.1"
                     /translation="MERTIVGIDIGTTKVCTLVGEVNASSDLRIVGVGIAPARGMKKG
                     VVVNVAEASAAIQASIEKAERTSGYEIARAFISVTGAHVSAINSRGVVAVARGERGIA
                     VDDIERALDAARAIAIPNDREVLHVIPRGFTVDGQNGVQDAIGMIGFRLEVEAHIITG
                     AASSIHNLLKCVEGCKVGVDGFVLDPIASGEAVLTDTEKQMGVVLADIGGGTTDIAIF
                     IEGSVWHTTVLAVGGNHITNDIAVGLRVPAEAAEQLKIEYGHARHETIDPTEVVQVKA
                     FGDGGVTRVSRRDLAEIIEARAEEIYQLVLQEIKRSGYDGLLPAGVVLTGGTAQLRGL
                     RELGRQVLGLPVRVGGPQNLNGLVDAIDSPAYATAVGLLLWAQRQGDRLPMSNGKRGD
                     APDLNTRFGNFFRKILPDRNS"
     gene            complement(6212..7012)
                     /locus_tag="VFF70_14275"
     CDS             complement(6212..7012)
                     /locus_tag="VFF70_14275"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZY45902.1"
                     /translation="MKSKPQARLKSRSRYLRYEANTTLIGSVQSAARIDFKSMLNIRS
                     VSIVLLLIVIGLALWLALDDRFYVNSIVITGNTRVSAADIAQKSGALGQHIFWLRPND
                     VAQYLPAALPSVRSAKMSCEFPADCSLSVVERQPLVAWQYGNAITWIDADHVAFAAEG
                     KAAGSLITIEATEGPALFPGQHADQQLIDAAIAVAQAMPEVRTYGYTAAHGLEFNAAR
                     GFPVYLGVGSNMADRAMVWKTLNPYLASKDIIPKFVDVRYPLAPYYGQ"
     gene            complement(7024..8136)
                     /locus_tag="VFF70_14280"
     CDS             complement(7024..8136)
                     /locus_tag="VFF70_14280"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZY45903.1"
                     /translation="MTKRKIRVGIIFGGKSAEHEVSINSAKNVMRALDKNKYEAIPIG
                     IGKDGRWLTRGDPLKALTGGESSLPQLLTLGYQIVDERSAGRELIPGTHASSLPEVDV
                     IFPVLHGPFGEDGTVQGLLELADIPYVGAGVMSSAVGMDKAAMKDLFRSHNLPTPKYA
                     IIKRREWEMTPELAAKKIASEIGYPCFVKPANMGSSVGVHKVHIVEELTAAMNDAASY
                     DRKIVVEHAVPNAREIECSVLGNDDPIASVVGEVVPSHEFYDYEAKYIDESSQLLIPA
                     PIDPTVAARVRDLSLQAFRALDCAGMARVDFLLERESNALYLNELNTIPGFTQISMYP
                     KLWAASGISYTELIDRLIDLALERHDDRHRLKTNYD"
     gene            complement(8219..9211)
                     /gene="murB"
                     /locus_tag="VFF70_14285"
     CDS             complement(8219..9211)
                     /gene="murB"
                     /locus_tag="VFF70_14285"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZY45904.1"
                     /translation="MQNAIDQLQSAVKGLRSKPEFKVNELLSRYTTARIGGPADVLIE
                     VASADELQQIVIAARTVSLPIFILGGGANVLVSDAGVLGLTIINKAKRIEFRDNGQVW
                     CESGTVLPTLARECIAHGLGGLEWAIGVPGTVGGAVVGNAGAHGRDIAADLVSASILD
                     EHDELVEMSNVELQYEYRSSRIKRSQKPVVRSRNENATPLRFATGGVTRNMSYVVLAA
                     TFQLTTESRSELEQKATEFNEYRRRTQPPGASIGSMFKNPIGNSAGRLIDQAGLKGMA
                     IGGAEISNIHANFFVNHGDAKASDVKALIDLAHERVQEKFGVELELEIELVGEW"
     gene            complement(9259..9456)
                     /locus_tag="VFF70_14290"
     CDS             complement(9259..9456)
                     /locus_tag="VFF70_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45905.1"
                     /translation="MTTNQSPKTTPSKKQTVSKFDQMFDEWRSGKQVGWSDLLAAWVE
                     APPAPRSPDLVIKVIRSMGAK"
     gene            complement(9475..10869)
                     /gene="murC"
                     /locus_tag="VFF70_14295"
     CDS             complement(9475..10869)
                     /gene="murC"
                     /locus_tag="VFF70_14295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZY45906.1"
                     /translation="MKPVILLPPNARLHIIGIGGAGMSAIAIVLMERGYQVSGSDQSE
                     SEVTRRLSDQGARVFIGHRAENVGEVDMVIASSAVHEDNLEFAAARSRGIPVSKRAQF
                     LSWLMQGSLGVAIAGTHGKTTTTAMITQILLGAGRDPSFIVGGTIGAIGKSAHAGRDR
                     EFVIEADEYDRTFLGITPTIAIITNIEHDHPDYYPTFDDVLAAFRSFIKLLPADGLLV
                     VCGEDVPATKLASSAPRKIVYGFDSKFDWYATDVRPNNAGGNDFLAYHQGESLGLVRL
                     RVPGEHNVLNALAALAATDRLGVPFGAAQDALSEFRGVGRRFEVRGEVKGVTIVDDYG
                     HHPTEIKATLAGARQRYPDRAIWAVFQPHTYSRTKTLLDQFAAAFVDADHVIVTAIYA
                     AREKDTLGISTADVVRSLDHTDARAIDALTDAVEYLRAHTKEGDVVITFSAGDANKIS
                     TEMINEQVTVNNDE"
     gene            complement(10866..11477)
                     /locus_tag="VFF70_14300"
     CDS             complement(10866..11477)
                     /locus_tag="VFF70_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45907.1"
                     /translation="MDPIEVLWGTIAVIFILVGLVRGFLKELGVTIVLIVMLFGYDRL
                     IPFLEDFIDKGGLLAIGVAPLNHDPSALTPTATGTLLWLVLTILTVLITFVAYQGETL
                     TYEGHNPRFPVGTFLSLVVGSVNAYLLTGTLWWILDRYHYPLQQIKLIDPTQLTDFAH
                     SILNGHLLPMDLLGNGAIPPTAGFGSVWLPLILVVLIVLKVLR"
BASE COUNT         2663 a         3479 c         2882 g         2636 t
ORIGIN      
        1 aaactcctgg ccttcggcgc tgccgtaacg aataccgaag tgatacatca tatcgcccag
       61 gcccatgatg ccgaggccaa tccgtcgggc gaggtaagcc gcgtctttta actgcggcac
      121 agccggcaca tatttattcg ccgtcacaac gttatccaga aaatgcgtgg agagaatcgt
      181 cgactctttc aacttctccc aatcaacttg cggcatgcca tcgactgtcc tcatatgctg
      241 agcgagattg atcgatccca agcagcaatt ttcgtaaggc cctagccatt gttcgccaca
      301 tggattggtt gcttcgagtg catataaatg cggcacggga ttcgttcgat tggccgcgtc
      361 gaggaacaac acgcccggct caccgttgcg atacgcattc ttgacgatgg cattgaagat
      421 ttcgcgggcg cgcagcgtct tcgtcaccga tccgtcatgc ggcgagatca gatcaaaatt
      481 ctgatcatcg cgcacggcgc gcatgaagtc gtcggtaatg ccgacggaga tgttgaagtt
      541 ggtaatctga tcttcactca ctttgcagtt gatgaagtcg agaatgtcgg gatgatgcac
      601 cggcaacaca gccatattcg cgccgcgtcg agtgttgtgg gtgactaaac cgtaagccag
      661 ataagtgtgg ttctcctcaa cttctagatc caacgtgagc gattcaccga tcggttccac
      721 accactcact tgaacaaacc atagcccgtc acctgagcgt gcatgttcag caaactccgg
      781 gtactggcga cgcaattcat cgtagcctga tcgcgtcagc tgtcgatcat tccgagaata
      841 gcgcagcagc gatcggcgca ggcgcggatt atcactgcgg aagttgagac ccatgcctcg
      901 gccttttgca tcgatctgtt ctagtgtgat cgcttccaac actggctcga tccaatagcg
      961 cgcatgcggc aatggataga cgctctcacg ctgcagaccg gtttgccagg cataagctgc
     1021 gacgaagcgc gagcgctgat cgcagccgat cttttcacgc caggcttgca agccgacact
     1081 cgattcgatg cggacgtgat agatgttcgc ctgaccgtaa tgattgacac cgggtgcaat
     1141 cgagtgaagt cgcaagggac aacccaagcc aatcaataat gcagcaactt cttcggccaa
     1201 ttggctcgag gtggtagtga gatcaggata gccgtggctc aaaccaccat cggcctcaaa
     1261 cagtccgcgc aagtatgcac cgacgatttc cgacggcgat tgccgaatca agcgcggcac
     1321 actgacatca cggctgcgcg ttttattgag gccattcatt tgtagaaatt ctttgagagc
     1381 gcgattatcg atcaccagtg ttacggatcg atcgtgcgat ttctgctgtc gatgaatctt
     1441 tacgccaaac agccgctcca gcatggcggg catttcttcg atcaggtacg aatcgtgcgc
     1501 cacgctcaca cccacacgat gatcgtcctc ggcggaagcc acgaatccat cgccagcgag
     1561 ataacccaag aagaaggcga attcttcatc caacacgctg ggtagtttag gcaaaatttg
     1621 attgccatgc tcaaacttgg gtcggcgcaa agcttgcagc ttgccacgct gttggcctaa
     1681 ctgtaccaat aaccaatcgc ccgatttcag ttcttgcaat tcacgccact gcggtccatc
     1741 atccgtcatc actttcacgc gatgctgata cgtcccggcc aagctcagac cgttgcgcgt
     1801 atgcacgcgc agcacgggcg cgacaccgtt gttgaacgat cgaggcgaag atcgccaacc
     1861 ctgatcggtg gcgacattca atcggtgatc ctgccaaccc gtcttatttg aatcgactat
     1921 ttcatccaag cgcagcaagc cgcgatccgt gaagaccaat gtgttcggca gtaggcagcc
     1981 gccctgcgcg atctccccaa aggctttatc gtatacgcgt aaaaatccga ccgggccagt
     2041 agcttgaccc gcactcgagg caacgtacga tcctttgtgg cgcaatcgac cgaaactgaa
     2101 gccattgcca ccacccgttt gttgaatcaa tgcggcatcc cgcagtgttt ggaaaattcc
     2161 cgcgctatcg cgacccatat catcggtaat cggtaacacg aagcacgcgg cgagttgccc
     2221 caatggcgtg cctgcgccgg tgaaagttgg cgagttcgga aagaaacgca aatcagtcag
     2281 caaacgataa aaagcacgct cggtcgcctc aacatcgaga ccgaattctt gatcgggttc
     2341 agccacggct tgtgccacgc gatggaacat gccttccggc gattcgatcg gctttccgcc
     2401 cggcccgcgc cgcacgtaac gcttcattaa taccacacgc gcattatccg tcaacttgac
     2461 ggcgcgatca tctgcttcaa gagtggtcgt tggggctgcc tgcttgcttt tcgacatcat
     2521 actcctccac aataaagttt ttcaaaacac tttcaaacaa aaacgcgtct cacgagaatg
     2581 cgcttaggca tcctcccaga gaacgcgcct attatcgttc gagaatcttg tcgatttcgc
     2641 gtcgaatgct ttcgacgtcg tctaaaccga gatacaccgt cgcaaagcga atgtaagcca
     2701 cttgatcgag cggcttcaaa ccgccgatca ccagatcgcc gatcatctta cccggcactt
     2761 cggctttgcc ggtctgttga atcttagcct cgatggagtt cactaagctt tcgagtgcat
     2821 cgctcgaaat aggccgcttg gcgcaggcca tgcgaatacc cgataacagc ttatcgcgat
     2881 cgaacggctc gcgtcgaccg tcgctcttca cgacgagcgg tgtcgtcaag atcgcacgct
     2941 cgaccgtgga gaaacgcttg ccgcagttgt tgcattcgcg gcggcggcgg atgcccggcc
     3001 cagtggtttc atgcgcggta tcgatcacgc gcgtatcatc gctttcgcag taaggacatt
     3061 tcatgatcta atgaagaaca agttttaaat gacagcttga ccggccgaac tgaatgatgt
     3121 cacatatcat acgctgatac tacatatggt acttcgagac tacaagaccc caagatgtgg
     3181 ggtctttatg cccgatcatc atagcacaga attttctaaa atcaatagag aaaccttaaa
     3241 aaaagatcga tcaaaccaaa ctgtaacatg gttgcgacat cgcgttcgac tattaaacca
     3301 aaaaagagaa ctccctcgat gaacatgaga accatcaagg gagttcaatg catgctaggt
     3361 tttcaggagg tgattcgaca catgcacgcg acgcgcgatc atatcatctc accaatgtga
     3421 gtcgtaaatc agcacgcatg gtacgacctc tctattgatc gggatagaga gtcgaacatc
     3481 caacgtcatt acgagacgcg catcctgagc atagcgcttt gcgcgaagtc gaaggacgcg
     3541 tcgaagaaat ctccgatcga atatgagatt gcttcgctgg caatgacgat cggccgcgtt
     3601 gatctatcgg cgcttgcgca agaacgccgg cacttccaga tcgtctttat cgaattggcg
     3661 tactggaaaa tcaaccggga tgcccaacga ttgatcggtc gtcgccatcg gtttgctgcc
     3721 cacagtgagc atcgacggct gcgctgaaac gctgacgcgt cggcggaccg gcgtcggccc
     3781 tgcctgatcg aaaccagtgg cgatcacggt cacgcgaatg gcatcgccca tcttgtcgtc
     3841 gatcaccgcg ccgaagatca gattgacatc cggatgcgcg gtttctttga tgatcgccgc
     3901 cgcctggttc acttcgtaga gcgtcatgtt cggaccgccg gtcacgttga acaagatgcc
     3961 gcgcgcgcca tcgatcgtca catccagcag cttgctggtg atggcttgct gggccgcttt
     4021 gatagcgcga tcttcgccct cggctgaacc gacggccatc aaggccgcgc cgccttcagc
     4081 catgatcgtc cgcacatcgg caaagtccaa gttgatcaaa ccgggcacgg tgatcaattc
     4141 actgatgcct tgaatgccct ggcgcaggac atcatcggcc acgcggaacg attgctgcag
     4201 cgtcgctttc ttgtcgacga tctccaacaa gcgatcgttg ggaatggcga tcaaggtatc
     4261 gacttgttcc ttcagcgcgt tgatgccgtt ctcggccgcg gccgatcgct tgtgaccctc
     4321 gaaggtaaat ggccgcgtca ccacgccgat cgttaacgcg ccgatttcac gcgcgatgcg
     4381 cgccacaatt gcgctggcgc cagtgccggt gccgccaccc atgccgcacg taacgaacac
     4441 catatcggcg ccgctcatgg cggcctgcag atcgtcggcc gattcttcag cggccttcgc
     4501 gccgacttcg ggattgccgc ccgcgcccaa accgcgcgtg agcttatcgc cgatacgcac
     4561 ttttgtcggt gcggtcgtca tcttcaacgc ttgcgcgtcg gtattgacag cgatgaaatc
     4621 cacgcccggc aatccttcgc cgatcatgcg attgactgcg ttcgtgccac cgccgcccac
     4681 gcccaccact ttgatgcgcg cgtattgctc tgcgttactg ctgacattcg attgcatttg
     4741 tctatcctcc tgaatgattt ctacatcgac acttcgagcg cggctgatcg atccctcaac
     4801 cgcgcgatcg agcttagcta ttccgatcgg gcagtatctt cctgaagaaa ttgccgaagc
     4861 gcgtgttcaa atccggtgca tcgccgcgct tgccgttcga catcggcagc cgatcgcctt
     4921 gccgctgcgc ccacaacagc agaccgaccg cggtggcata tgcgggtgaa tcgatcgcat
     4981 cgaccaaccc gtttaagttt tgcggtccgc ccacgcgcac gggcaagccc aggacttgtc
     5041 ggcccaattc acgcaggccg cgcaattgcg ctgtgccgcc ggtcagcacg acgcccgcgg
     5101 gcaacagccc gtcatatccc gatcttttga tctcctgcag aacaagttga taaatttctt
     5161 ccgcgcgcgc ttcgataatc tcagccagat cgcggcgcga cacgcgcgtc acaccgccat
     5221 cgccaaacgc tttcacttga accacttcag tcggatcgat cgtttcatgc cgagcatgac
     5281 catactcgat cttcagctgc tcagccgctt cggcgggcac acgcaggcca acggcaatat
     5341 cgttggtaat gtgattgccc cccaccgcca gcaccgtggt atgccacaca ctgccttcaa
     5401 taaagattgc aatgtcggta gtgccgccgc cgatatcggc cagcaccaca cccatttgtt
     5461 tttccgtatc ggtcaacacg gcttcaccag acgcaatcgg atccagcacg aaaccatcca
     5521 cgccgacttt gcacccttcc acgcacttca acaaattgtg gatcgatgaa gccgcgcccg
     5581 taatgatgtg cgcctccact tccaaacgga aaccgatcat gccgatcgca tcctgcacgc
     5641 cgttttggcc atcgaccgtg aagccgcgcg gaataacgtg caacacttca cgatcattcg
     5701 gaatcgcgat cgcgcgggca gcgtccaaag cgcgctcaat gtcatcgacc gcaatgccgc
     5761 gctcgccgcg tgcgaccgcg acgacaccac gtgaattgat cgcgctgacg tgcgcgccgg
     5821 tcacactgat gaacgcgcgc gcgatttcgt aaccgcttgt tcgctcggct ttctcaatcg
     5881 aagcctggat cgcggcgctg gcttcggcta cattaacgac cacgcccttc ttcatgccgc
     5941 gcgcaggcgc aataccgacg ccgacaatgc gcaggtcgct gctcgcgttg acttcgccca
     6001 cgagcgtgca gactttggtg gtgccaatat caatgccgac gatagttcgt tccactttta
     6061 cctcaaggtt taattcttat cgctcaagac ttaaacggct aagaatgatt caaattgtaa
     6121 ctggcttaaa gtctgcgttc gacaatcggt ccaagccagt gtgcagtcgg tattgttcat
     6181 cgtgtattct gcagcctgca cgacagtttc tttattgtcc atagtatggc gctaacgggt
     6241 aacgcacatc gacaaatttt ggtatgatgt ctttcgatgc caaatacgga tttaatgttt
     6301 tccagaccat cgcccgatcg gccatgtttg aacctacacc cagatacact ggaaagccgc
     6361 gcgcagcatt aaattcaagg ccatgtgcgg ccgtgtaacc ataagtgcga acttccggca
     6421 tcgcttgggc caccgctatc gccgcatcga tcaattgttg atcggcatgt tggccgggaa
     6481 acaacgccgg accttcggtg gcctcgatcg tgatcagcga tccagcagct ttgccttcgg
     6541 cggcaaaagc gacgtgatcc gcatcgatcc atgtaatcgc attgccatac tgccacgcca
     6601 cgagcggctg tcgctcgaca acgctcaggc tgcaatcggc cggaaactcg caggacatct
     6661 tcgcgctgcg aactgagggc agtgccgcgg gcagatactg tgccacatca ttcggcctga
     6721 gccagaaaat atgttggccc aatgcaccgc ttttctgcgc gatgtccgcc gcactcacgc
     6781 gcgtgttgcc cgtgatcaca atcgaattca catagaatcg atcgtcaagt gcgagccata
     6841 gagctaaacc aatcacgatc aacaacagca cgatactcac cgagcgaata ttcaacatcg
     6901 acttgaagtc aatgcgcgcc gccgattgaa cactgccgat taacgttgta ttcgcctcgt
     6961 agcggagata gcgtgatcgt gattttagcc gggcttgtgg tttcgatttc atttttcaac
     7021 tcatcaatcg taattcgtct tcaggcgatg ccggtcgtca tgccgctcta aggccagatc
     7081 aatcaagcga tcgatcaatt cagtgtatga aatgccgctg gccgcccaca acttgggata
     7141 catactgatc tgagtgaagc cgggaatcgt attgagttca ttaagataca gtgcgttcga
     7201 ttcacgctcg agcaaaaaat cgacgcgggc catgcccgca caatccagcg cgcggaaagc
     7261 ctgcagcgat agatcgcgca ctcgagcggc gaccgtcgga tcgatcgggg ccgggatcag
     7321 cagctgcgag gattcatcaa tgtacttcgc ttcgtaatcg tagaactcgt gactcggcac
     7381 cacttcaccg acaacagacg caatcggatc gtcattgcca agcacactgc attcaatttc
     7441 gcgcgcattc ggcacagcat gctccactac tattttgcga tcgtaagacg ctgcatcatt
     7501 cattgctgca gtcaattctt caacaatatg tactttatgc acacccacgc tcgatcccat
     7561 gttggccggt ttcacaaaac acggataacc aatctcactc gcaatttttt ttgcggctaa
     7621 ctccggcgtc atctcccatt cacggcgttt gatgatggcg tacttcggcg tcggcagatt
     7681 gtgactgcgg aacaaatcct tcatcgcggc tttgtccatg cccactgcac tcgacatcac
     7741 ccccgcgccc acatacggaa tgtcggccaa ctccaacaag ccttgcactg ttccgtcttc
     7801 gccgaacggg ccgtgtagca ctggaaagat cacatcgacc tcaggcaggc tggaagcatg
     7861 cgtgccggga atcaattcgc gtccagccga tcgttcatca acaatttgat aacccaacgt
     7921 caacaactgc ggcaacgatg actcgccgcc ggtcaacgct ttcaaaggat cgccgcgcgt
     7981 caaccatctg ccgtccttcc caatgccaat cggtatcgct tcgtatttat ttttatcaag
     8041 tgcgcgcatc acattcttgg ccgagttgat cgaaacttcg tgctcggctg atttcccgcc
     8101 aaaaatgatg cccacgcgga tttttcgttt ggtcatttgt taatcaggat ccaagttgca
     8161 agatgcaaat gagcaaaatc accacgtcat cttatcgcct tgttagcgtg tcagcctttc
     8221 accattcacc taccaactca atctccaact ccaactccac accaaacttt tcctgtactc
     8281 gttcatgcgc cagatcgatc agtgccttta catccgaggc ttttgcatcg ccatgattca
     8341 caaagaagtt cgcatgaatg tttgaaatct ctgcgccacc gatcgccatg cctttcaagc
     8401 ccgcttgatc gatcaaccgt ccggctgaat tgccgatcgg attcttgaac atcgatccga
     8461 tactcgcgcc cggcggctgc gttcgtcgtc gatactcgtt gaactctgtg gccttctgct
     8521 ccaactccga tcgagattca gtagttaatt gaaatgtcgc cgccaggaca acataggaca
     8581 tgttgcgtgt gacacccccc gttgcgaagc ggagtggggt tgcgttttca ttccggcttc
     8641 tgactactgg cttctgactt ctctttattc ggctgctccg atactcatac tgcagctcta
     8701 cgttcgacat ctcgaccagt tcatcatgtt catccaagat gctggccgaa actaaatccg
     8761 ctgcgatgtc gcgtccatgt gctccggcat tacccacaac cgcgccgccc accgtgcctg
     8821 gcacaccgat cgcccattcc agtccaccga gcccgtgtgc gatacattcg cgcgccagtg
     8881 tgggcaggac ggttcccgat tcacaccaca cttggccgtt atcacgaaac tcaatccgtt
     8941 ttgctttgtt aatgatcgtc aggccgagta cacctgcatc gctgactaac acattcgctc
     9001 cgccgccgag aataaagatc ggcagtgaaa ccgtgcgcgc cgcaatgaca atctgctgca
     9061 actcatcagc gctggctact tcaatcaaca catccgccgg accaccgatg cgcgccgtcg
     9121 tgtagcgcga taacaattca tttaccttga actccggttt cgaccgcaga cctttgaccg
     9181 ccgactgcag ttgatcgatt gcattttgca tcgcacctct tgcgtcctgc atcttgcctc
     9241 ttgcctgatc cctgatccct attttgcgcc catacttcgg atcactttga tcaccagatc
     9301 gggcgagcgt ggcgcgggcg gcgcttcgac ccaagccgcg agcagatcag accaaccgac
     9361 ttgtttgccg gaccgccact catcgaacat ctgatcgaac ttgctgacag tttgtttttt
     9421 tgatggcgtg gtctttggac tctgattagt tgtcatgact cctccgatcg tttgtcattc
     9481 gtcattgttc acggtgactt gctcattgat catttccgta ctgatcttgt tcgcatctcc
     9541 agcactgaaa gtgataacca catcaccctc ttttgtatgg gcgcgcaaat actcaaccgc
     9601 atccgtcaac gcatcgatcg cgcgcgcgtc cgtatgatcc aatgaacgga cgacatcggc
     9661 agtgctgatc ccgagggtgt ctttttcgcg cgcagcataa atagccgtga cgatgacatg
     9721 atccgcatcg acaaacgccg ccgcaaattg atcgagcaag gtcttggtgc ggctgtaggt
     9781 gtgcggctgg aacacagccc agatcgctcg atcgggatag cgttggcgcg ccccagccag
     9841 cgtcgctttg atctccgtgg gatgatgacc gtaatcatcg acaatcgtga caccctttac
     9901 ctcgccgcgc acttcgaagc ggcggcccac accgcgaaat tcactcagtg catcctgcgc
     9961 agcgccgaag ggcacgccta atcgatcggt cgccgctaaa gccgctaaag catttaacac
    10021 attgtgttcg cccggtaccc gcaatcgaac taaacccagc gactcgccct gatgatacgc
    10081 caggaagtca ttgccaccgg cattgttcgg ccggacatcg gtggcatacc aatcgaattt
    10141 agaatcaaag ccgtacacaa tttttcgagg ggcgctgctc gctaatttag ttgccggcac
    10201 atcttcaccg catacgacca gtagaccatc ggcgggcagc aacttgatga acgatcgaaa
    10261 cgctgccaac acgtcgtcga aggtcggata atagtcggga tgatcatgct cgatgttggt
    10321 gatgatcgcg atcgtgggag tgatgcccaa gaaagttcga tcgtattcat cggcttcgat
    10381 cacgaactcg cggtcgcgtc cggcatgcgc ggattttccg attgcgccga tcgtgccacc
    10441 gacaatgaaa ctgggatcgc gacccgcgcc taacaagatc tgcgtaatca tggccgtcgt
    10501 cgtcgttttg ccatgcgtcc cagcgatggc gacgccgagc gaaccttgca tcagccatga
    10561 caaaaattgc gcgcgcttgc tgacgggaat gccgcgtgat cgggcagccg cgaattccag
    10621 gttgtcttca tggacggcgg aggaagcaat caccatatcg acctcgccca cattctcggc
    10681 gcggtgtccg atgaataccc gcgcgccttg atcgctcaac cgtcgtgtga cctctgattc
    10741 agattgatcc gagccgctca cttgataacc tcgctccatc aagacaatcg cgatcgcgct
    10801 catcccggcg ccgcctatgc cgatgatgtg caatcgcgcg ttgggcggca gcaaaatcac
    10861 cggcttcatc gcaaaacctt cagcacaatc aagacgacta agatcaacgg cagccagacc
    10921 gagccaaagc cggccgttgg cggaatcgca ccattgccca gcaagtccat cggcagcaga
    10981 tgaccgttca agatcgaatg tgcgaagtcg gtcaactggg tgggatcgat caactttatc
    11041 tgctgcaacg gataatgata tcgatccaga atccaccata aagtaccggt gaggagatag
    11101 gcattgactg agccgacgac caaacttaaa aacgtgccga ccggaaatct gggattatgg
    11161 ccttcatacg tcagtgtttc accttggtaa gcaacgaacg taattagtac ggtcaatatc
    11221 gtcaacacca gccacaagag tgtgcccgtc gcagtcggtg ttagagcgct cgggtcatga
    11281 ttcaatggtg ccacaccaat ggccagcaag ccgcccttgt caataaagtc ttccaaaaac
    11341 gggatcagtc gatcgtagcc aaacagcatc acgatgagta cgatggtgac gccgagttcc
    11401 tttaagaagc cgcgcaccaa accgaccaaa ataaaaatga ctgcgattgt tccccacaga
    11461 acttcgattg gatccataat gttttagttt gcctcagtcg atcggccgtt cagcccacca
    11521 atgtccttaa cctttttacc gcttcttcgc cggcgggcgt gaccgctgca caccgaacac
    11581 aatcaacacc atgaccagcg ccaagatcaa cggcatgttc accgtcagct gcggcacatc
    11641 cgtagccgtg atcttaatgt
//
LOCUS       DASRTJ010000706         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_144721,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000706 DASRTJ010000000
VERSION     DASRTJ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144721"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..560)
                     /locus_tag="VFF70_14305"
     CDS             complement(57..560)
                     /locus_tag="VFF70_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45908.1"
                     /translation="MTPSDSSTTSSDRATAISTDSTLGNGALPKRSAIEPTNAYILVV
                     EDNIQNFVLIARLMAYLGVKQCQWKASGWQVLEFADSMPRVDLILMDIHLPYEDGFEA
                     LARLRSEPRFKDTLIVAVTADANETTLKQAKQSGFDGFIGKPIDPDRFPEQVKMILRG
                     EPVWSFR"
     gene            complement(557..934)
                     /locus_tag="VFF70_14310"
     CDS             complement(557..934)
                     /locus_tag="VFF70_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZY45909.1"
                     /translation="MTKQILYIEDNSDSRTLVRRVLQSEGYVVREAPDGRTGLQMAAE
                     QRPDLVLMDINLPEVDGYEVTARLKQLAGMSQVPVIAVTANVMKGDREKTLAAGCDGY
                     IQKPIDIDTLSNQIEHFLTRNNS"
     gene            complement(1021..1146)
                     /locus_tag="VFF70_14315"
     CDS             complement(1021..1146)
                     /locus_tag="VFF70_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45910.1"
                     /translation="MVDSGSMVVDDDERTKSFFCDSLVALDITDRAIGAASAKEA"
     gene            complement(1294..2100)
                     /locus_tag="VFF70_14320"
     CDS             complement(1294..2100)
                     /locus_tag="VFF70_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45911.1"
                     /translation="MLQGTHLLIVDDERTTRLSLSEIFTLRGAIVSMAADGLEALAKI
                     AEAEYDLIILDIKMPGASGLQVLEEVQKKSPATIVILLTAHATVDSAITALRKGAFDY
                     ILKPAQPRSIIEVVERGLAKRQDHLRKQNLVGLMAQTVEAFKDNTGQPSLVSAPVDSV
                     TDRQVTIGSIGVDFDRHEARVADRLLDLTPTEFDTLAYLVRHAERVVSCRDMVQAVHG
                     YETTEHAARPVMRVHIHRLRQKIEADAAKPEYLLTIRSAGYMLVSQPRGK"
     gene            complement(2319..>4373)
                     /locus_tag="VFF70_14325"
     CDS             complement(2319..>4373)
                     /locus_tag="VFF70_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45912.1"
                     /translation="DVARTWHQLLGVIDATNWPARDPIVLHADTTPTIDSIGGSDRAL
                     GIRAWISVPLRQRGTLNGLLVLASRAANKFDQSETTLIGTVGAEISVAITNSRLYEET
                     RQRVEELSLLNEVSRMLTSTLDLDKVLRVIMEATVSMLQGEAGSVLLLDEATGDLVFA
                     AAVGPDAESIVGLRLPAGQGIAQRALTEDHALLIDDVQQDDNFYPGIDRVTSLTTRRI
                     IAAPLRTHGRLIGVMEIINKRRAEFSDADLRILDLLTPTAAAAIENARLYARDTQLSA
                     EVRRRNRELSALHALSAALSQSLEITSVLDISLVMIQPLFDYAEGSIALLDHDALNYL
                     ATHSATPHSLPAPDRAELADRFSREAIDAHAVKIIARIDQLDDRLAASWRQAGIGAMA
                     AVPLWGHDHVQGVLQMIWHAARDFTDETAPLLAAMGQQIGVAIERAHLYEVAQRRAKE
                     IEQSYALLVQSEKLAATGRLALSLAHEINNPLQAIQNCLHLALEFDLNETKRTEYLKL
                     AREEVERLSILVQRMLDFYRPAPEAQTNADVRVVLERVLALSEQKLHHNDVELKLEIP
                     TRQLLVQAAPDQMAQVFLNLIVNAAEAMEAGGQLKISSTANGDWIETRFEDNGPGIPS
                     EQLPHIFEPFFTTKSDGTGLGLAISYTIVEHAGGSISVDSRSGEGTTFVVRLPKAAEK
                     SD"
BASE COUNT          952 a         1189 c         1219 g         1013 t
ORIGIN      
        1 gacggagtca ggctggatag cattctgcct cacaaaacta gcgaagatcg atcggattaa
       61 cggaacgacc agacgggttc accccgcagg atcatcttga cttgctccgg aaagcgatcg
      121 ggatcgatcg gtttgccgat aaagccatca aagcccgatt gtttcgcctg cttcagggtg
      181 gtttcattgg catccgccgt gacagcgacg atcaatgtat ccttgaaccg cggttcgcta
      241 cggaggcgtg ccagggcttc aaagccatct tcatatggca gatgaatatc catcaaaatc
      301 aaatcgacgc gcggcatact gtccgcaaat tccaatactt gccagcccga ggctttccac
      361 tggcattgct tcacgcccag ataagccatg agccgcgcaa tgaggacaaa gttttgaata
      421 ttatcttcca ccacgagaat gtaggcattg gtcggttcga tcgccgagcg tttgggcagg
      481 gcgccattgc ccagtgtgct atcagttgaa atggccgttg ctcgatcgga agatgtcgtg
      541 gacgagtctg aaggtgtcat gagttattcc ttgtcaagaa gtgctcgatt tgattcgata
      601 acgtatcaat gtcgatgggc ttttgaatgt aaccgtcaca accggccgcc aaagtttttt
      661 cacgatcgcc tttcatgacg tttgcggtga cagcaatcac tggcacttga ctcatgccgg
      721 ccagctgctt taaacgggcg gtgacttcat aaccgtcgac ctccggcaag ttgatatcca
      781 tcaacaccag atcgggtcgc tgttcggcgg ccatctgcag accggtacgg ccatctggcg
      841 cttcacgcac aacgtagccc tcagattgaa gcacgcgccg gacgagcgtg cgactatcgg
      901 agttgtcttc gatgtagaga atttgtttag tcatttttca ccatccacac ctgcctttag
      961 tgagataggc gtcactggcc gggtcttcta aataagtgaa gtaggcgccg ttgagttcga
     1021 tcaggcttct ttagccgacg cagcgccgat cgcgcgatcg gtaatatcga gcgcgactag
     1081 gctatcgcag aaaaacgact ttgtgcgctc atcgtcgtcg acgaccatgc tgcctgaatc
     1141 gaccatgctc tccttaaagc cctttgggga tgatcttaga gtagcataca gccgatccaa
     1201 attgatttct ggatgattgc aaatttattg cggaagactt tcaatttgag gctggaacct
     1261 gttgtgtggg cgtgcgacgg cacgtgtcgg ggactatttg ccgcgcggct gcgagaccag
     1321 catataaccg gctgatcgaa tggtgaggag gtattcaggc ttggcggcat cggcttcgat
     1381 cttctgccgg agccgatgaa tgtggacacg catgacaggt cgggcggcgt gctcggtcgt
     1441 ttcataaccg tgaacggctt ggaccatatc ccgacagctc acgacgcgtt cggcatgccg
     1501 cacgagataa gccaacgtgt caaattcggt cggcgttagg tctagcaagc gatcggcgac
     1561 gcgggcttca tgccgatcaa aatcgacgcc gatggaaccg atcgttacct gtcgatcagt
     1621 gactgaatcg accggagcac tgacaagtga cggctgccca gtattgtcct tgaaagcttc
     1681 aaccgtctga gccatcaatc cgaccaaatt ctgttttcgc aggtgatcct gccgcttagc
     1741 aaggccgcgt tcgaccactt cgatgatcga gcgcggttga gcgggcttga gaatgtaatc
     1801 gaacgcgcct ttgcgcaagg cggtgatcgc cgaatcgacc gtggcgtggg cggtaagcag
     1861 gatcacaatg gtggccggtg attttttctg aacctcttcc agaacttgca atccgctggc
     1921 gccgggcatc ttgatatcca aaatgatcaa atcatattcg gcctcggcga tcttggccag
     1981 cgcttccaat ccgtcggcag ccatggagac gatcgcgcct cgaagcgtga agatttcgga
     2041 caaagataat cgcgttgttc gttcgtcgtc gacaatgagg aggtgtgtgc cttgtaacat
     2101 agttgatcag agatcagttt ttcagggatt agggattaat caattaggga ttaggaactc
     2161 accttcagca gtgtcgcacc cgcgaaggcg ggtgtccagt cgattagctc tggattcccg
     2221 ccttcgcggg aatgacaaac aacttcattt gggtactgtg atagatatga gtttaacatg
     2281 atatccattg gcaacaaaat acggatcatc cgccatctct aatcactctt ctccgctgct
     2341 ttcggcaatc gcactacaaa cgttgtgcct tcacctgatc ggctgtccac gctgatcgat
     2401 ccgccagcgt gctcaacaat tgtatagcta atcgccagtc ccagaccggt gccgtcactc
     2461 ttggtcgtaa agaacggttc aaagatgtgc ggcagttgtt cggatggaat gccaggtcca
     2521 ttatcctcga agcgagtctc gatccagtcg ccgtttgccg ttgaagaaat tttaagttgg
     2581 ccgcccgcct ccatcgcttc agcggcattc acgatcaagt tcaagaacac ctgagccatt
     2641 tgatccggtg cagcttgaac cagcagttga cgcgttggta tttcaagttt cagctcaacg
     2701 tcattgtggt gcagtttttg ttctgatagt gccagcacgc gttcgagcac aactcgcaca
     2761 tcggcgttgg tctgagcttc cggtgccggt cgatagaagt cgagcatacg ctgcaccaga
     2821 atactcagtc gttccacttc ttcacgtgcg agcttcaggt actctgttct tttagtttcg
     2881 ttcagatcga actccagggc caaatgcaaa caattctgaa tcgcttgcaa tggattgttg
     2941 atctcgtgcg ccagcgacag agccaagcgg ccggtggcgg ctagcttttc cgactgcacc
     3001 aatagtgcat aagattgttc gatctccttg gcacggcgct gggctacttc atataaatgc
     3061 gcgcgctcga tggcgacgcc gatctgttgt cccatggcgg ccagcagcgg cgcggtctca
     3121 tcggtgaagt cacgcgcggc gtgccagatc atttgcagca caccttgaac gtgatcgtgt
     3181 ccccacaacg gcaccgccgc catcgcaccg atgccagcct gacgccaact ggccgccaat
     3241 cgatcatcca gctgatcgat ccgggcgatg atcttgacgg catgcgcgtc gatggcttcg
     3301 cgtgaaaacc gatcggccag ctcagcccga tcgggcgcag gtaagctgtg cggtgttgct
     3361 gagtgagttg ccagatagtt taatgcatca tggtcaagca gcgcgatcga tccttccgca
     3421 taatcgaaca acggttggat catcacgagc gagatgtcca acacgctggt gatttccagc
     3481 gattggctaa gcgcggccga gagggcgtgc agcgcactga gttcgcgatt gcggcggcgg
     3541 acctccgcgc tgagctgcgt gtcacgcgca tacagccgcg cattttcaat tgcggcggcg
     3601 gcggtcggcg tcaacagatc gaggatgcgc agatcggcat cggaaaattc ggcgcggcgc
     3661 ttgttaatga tctccatgac accgatgagg cggccatgtg tgcgtaaggg cgcggcgatt
     3721 atgcggcgag tggtcaggct ggtgacacga tcgatgcccg gataaaaatt atcatcttgc
     3781 tgcacgtcat cgatcaacaa cgcatggtct tcggtcagtg cgcgctgcgc aatgccctgg
     3841 ccggccggta aacgcaagcc cacaatcgat tcggcgtcag ggccgaccgc ggcggcaaag
     3901 accagatcgc cagtggcttc atcgagcagc agcaccgatc cggcttcacc ttgcagcatg
     3961 ctgacggtcg cttccatgat cacgcgcagc actttatcca gatcgagcgt agaggtcagc
     4021 atgcggctga cttcattgag taagctcaac tcttctacgc gctgccgcgt ttcttcatac
     4081 aagcgcgagt tggtgatggc gacgctgatc tctgcgccca cagtgccgat gagcgtcgtt
     4141 tcagattgat cgaatttgtt ggctgcgcgg ctggcgagca ccagtaaacc attcaaggtg
     4201 ccgcgctggc gcagcggcac gctgatccag gcgcgaatgc ccagcgcgcg atcagacccg
     4261 ccgatcgaat cgatcgtcgg cgtcgtatcg gcatgcagca caatcgggtc acgcgccggc
     4321 caattggtcg catcgatcac gcctagcagt tggtgccaag tgcgggccac atc
//
LOCUS       DASRTJ010000707         7188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_144759,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000707 DASRTJ010000000
VERSION     DASRTJ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144759"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2859)
                     /locus_tag="VFF70_14330"
     CDS             complement(<1..2859)
                     /locus_tag="VFF70_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45913.1"
                     /translation="MTELPSPRKTRSLDEPVAIILGIATIINSVRIVTEAPQPVLSPW
                     LYMIWIVTLAIAANLGISFESSDANFASLIIVAAFLSLGLVPAVIVTVTGIVLSEVLR
                     VLFPRPFKTRQRTHWRIFVVLFSNIAIYGLSVIGGGKLYLALGGVAPSPLLQANLTPA
                     SNATFNQLIVPILGLFTGYALINYFLFGLLFKLAGGQSVREYIRQHGRSVAILELAPL
                     WTATIIAYTAANISIPGFGAFCVFLITIMAIIHSMSDARTRLEKRVQEISSLSAVGQA
                     VANSLDLPTVLETIYEETRRLMTVRSFYVALYNDQDQTISFPLAYERGQRKNYPTRSF
                     SHGLTEYVIETGRPLLIKDRLRERLPKVEPILTGAAPKSWLGVPILSADQILGVMVAQ
                     DVEQANVYNEEHRNILTAIASQAAVAIQNAQMFATTQQHAAGLAMLNLVSTAISSTLD
                     LPELLNIIVTSAGQVMGNQKAAIFLTDESGQLLNLVAAHGLSAEYIAQSQAIPIIAKD
                     RSLAAFNKQPLLISDVRTEPNLIDYRPRAEAESIRALADVPLLTRGQAIGALTMYFTE
                     PHQFTLTEIDLLTTYANQAAAAVANAKLYAQTDQALAQRVDQLAALEEIGRDLSASLD
                     FDQVIQRVLERALAATGADFGSISLFDVDQQRVKVMVTHGYTPEATGQLMNQHWPVNH
                     GIVGRAIRSGQAIRINDVSSEPDYIAVDPQVRSELALPIQRDHTIIGAINLESTKPDT
                     FTPASIEFVSQLAIQSAVALQNAQLYQQAQQRLKETTILYSVGQQLTSILDLRQLGQA
                     LVERMALAIGTTICSLEILDPESNLVESIAQYFSPDFAHSPAALRLDRFYQLYDFPAT
                     LVSFQNRQPLIVYVSDPAADPKEVAALQQFGIQAMVTIPLSHGDEVLGAVEWADERSV
                     RRFSNDELRLAVTLANQAAIAVKNAQLFEERTQRITEFS"
     gene            3038..4084
                     /locus_tag="VFF70_14335"
     CDS             3038..4084
                     /locus_tag="VFF70_14335"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZY45914.1"
                     /translation="MNRHYLPLIRHSSHLHSNARSAVQVLLITRYALLSMFLAACGGQ
                     IISGPTPTPHSLAVILPSPTSISTLSPRKLTPDPTETPAPTPTPVIYIVEPGDTLLGI
                     ALQYNVTVDELQAVNGVLSPETLQIGQKLIIPVGGFSVPSSSGGIQLPSPTPVGIEVQ
                     HSALYQTLVGSSWVLGEVFNPGSIALENVEVRVALLDATGQEVAFDSEFVALEAIPPG
                     GRSPFGVLFANPPAVVVAFQATAIRAEPSQNVAARYVQLHVTDPQPKLVESIYHLTGS
                     IVNGTSSNAVEVHVVATIYDADRHVVGYRRSIWGDGSIGAGVSAPFDISVSPDSSAKP
                     VADYSIVAQGRVGQ"
     gene            4168..4893
                     /locus_tag="VFF70_14340"
     CDS             4168..4893
                     /locus_tag="VFF70_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZY45915.1"
                     /translation="MSTNLTDLNPLPRHTTPGWQRLLRFFARMIMLPVARIDVQGMEH
                     VPPDAFMGVTNHMSSFDTLALIAIGPIRRIALFAAIEHRSDFIAGWALNQLRVIWLRR
                     GEADREAIKIALDEIKSGSAFGIAIEGTRSKTGALLEGKTGAAYLATRANIPILPAVI
                     WGTEKIKHNLRKFKRTTVHIRIGEPFHLPEGRAGTEQLQQYTNQIMLKMASMLPSEYW
                     GVYAERMKEQLAMNNDQLAIPLR"
     gene            complement(4939..5778)
                     /gene="rsmA"
                     /locus_tag="VFF70_14345"
     CDS             complement(4939..5778)
                     /gene="rsmA"
                     /locus_tag="VFF70_14345"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZY45916.1"
                     /translation="MLEPRQLLKQFGLRPKKSLGQNFLIDNPALDKIVRAADVTADDV
                     VLEIGPGLGSLTQHLAAIAKRVIAVEIDGNLIPVLHSQLADRSNVEIVQGDILKIDPA
                     TLLREASTYKVVANIPYYITSGIIRHLLEASITPSLLVLTIQQEVAERIIAQPDDMNL
                     LAVSVQFYGSPRIVTNIQAGAFYPAPKVDSAVVRIDLDAKPRVEVKSVDTFFSVVKAG
                     FSQKRKQIHNSLIGGLGKPREEIDRLLQAGNIDPKRRAETLTLEEWAGLANLVDQGSG
                     IGK"
     gene            complement(5885..6922)
                     /locus_tag="VFF70_14350"
     CDS             complement(5885..6922)
                     /locus_tag="VFF70_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HZY45917.1"
                     /translation="MTRVCPSILSVIDLKRVTIEIKRAVPIEIKIGTNAQKFESAADT
                     IGQALAEANVQVYLADRITPDLATPLQSNLQVVIEPSMPINVQVDGQLLKTRTRRSTV
                     GEVLNDLGVTLLGEDYTQPTVDASVESGAVVNVVRVREEFLIQQEPIPFNTLTVADDS
                     MELDTSSVTAGQSGVKQKRIRVRFENGLEVARNVADEFVLQPPESKVYRYGTQIVVRT
                     LDTPDGPIQYWRHLRAHATSYSASTAGTPSTAKYYGRTVTGQQMRKGVIAVDKSVILL
                     FTNMYVPGYGFGQALDTGSAIQGKRLDLGYDDNNLVLWYRWVDVYLLAPAPSPERITY
                     VLPDWPPETGR"
     gene            6879..>7188
                     /locus_tag="VFF70_14355"
     CDS             6879..>7188
                     /locus_tag="VFF70_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HZY45918.1"
                     /translation="MRSITLKIDGQTRVIRTNQRSIENILRDADITIQPEDLVVPDYS
                     ATLDDRQSAITVQHARSIAIDVDGQTKQFRSRATALDELFKQANIEVGAHDRISVNGR
                     V"
BASE COUNT         1634 a         1906 c         2009 g         1639 t
ORIGIN      
        1 gctgaattca gtgatgcgct gggtccgttc ttcaaatagc tgcgcatttt tgacggcgat
       61 ggcggcttga ttggctagcg tcacggctaa gcgcaattca tcgttgctga aacgccgaac
      121 tgatcgttca tcagcccact ccacggcgcc cagcacttca tcaccatgac tgagcggaat
      181 ggtcaccatc gcttgaatcc cgaactgctg cagtgcagcc acttccttgg gatcggcggc
      241 cggatcggac acgtagacaa tcagcggctg gcggttctgg aaagatacca gggtggctgg
      301 aaagtcatag agctgataaa aacgatcgag gcgcagcgcc gccggactgt gcgcaaaatc
      361 cggcgagaaa tactgggcga tggattcgac cagattggat tcaggatcca gtatttccag
      421 cgagcaaatg gtggtgccaa tcgccagagc catgcgttcg accaacgctt ggcctaactg
      481 ccggagatcc aaaatcgagg tcagctgctg gccgactgaa tacaaaatgg ttgtttcttt
      541 caagcgctgc tgggcttgct ggtagagctg tgcgttttgc aaagcgacag ccgattgaat
      601 tgccagctgc gatacaaact caatgctggc gggtgtgaac gtatccggtt tggtgctttc
      661 caaattaatg gcgccaatga tcgtgtgatc gcgctgaatg gggagcgcta actcggatcg
      721 aacttgggga tcgacggcga tgtaatccgg ttcgctactc acgtcattaa tgcgaatggc
      781 ttgacccgat cgaatcgcgc ggcccacgat tccgtggttg actggccagt gttgattcat
      841 gagttgaccg gtagcctccg gggtgtagcc gtgcgtcacc atgactttga ccctttgttg
      901 atccacatca aataaactga tgctgccgaa atcagcgccg gtcgccgcga gcgcgcgttc
      961 cagcacgcgc tggatgactt gatcgaaatc cagggaggcg gacagatcgc ggccaatttc
     1021 ttccagcgcg gccagctgat cgacgcgttg agccagagct tgatcggttt gggcgtatag
     1081 cttggcgttg gcgactgccg cagctgcttg attggcgtaa gtggtcagta gatcgatctc
     1141 tgtcaaggta aattgatggg gttcggtgaa atacattgtc agcgcgccaa tcgcctggcc
     1201 gcgagtgagc aatggcacat cggcgagggc gcggatgctt tcggcctcag cccggggacg
     1261 ataatcaatc aagttgggct cagtgcgaac atcactgatc agcaaaggtt gtttattgaa
     1321 ggcggccaac gatcgatctt tcgctattat cgggatggcc tgtgactgcg caatatattc
     1381 ggcgctcaag ccatgcgccg cgaccagatt aagcagctgg ccggattcgt cggtcaagaa
     1441 gatggccgct ttttgattgc ccatgacttg gccggccgag gtcacaatga tgttgagcaa
     1501 ttccggcaga tcgagcgtgg agctgatggc ggtcgacact aagttgagca tcgccagccc
     1561 ggcggcatgt tgttgagtgg tggcaaacat ctgcgcattt tgaatcgcca cggcggcttg
     1621 ggaggcgatg gcggtcagaa tgttacgatg ttcttcattg taaacattgg cttgctcgac
     1681 atcctgtgcc accatgacac ctaagatctg atcagcagat agaatcggca cgcccagcca
     1741 ggatttgggc gcagcaccgg ttagaatggg ttccactttc ggcagtcgtt cgcgcaatcg
     1801 atctttgatt aacagcggcc ggccagtttc gatgacatat tcggttaaac catggctgaa
     1861 tgaacgcgta ggataatttt tgcgttggcc gcgctcgtag gccaatggga agctgatcgt
     1921 ttgatcttga tcgttataca atgcgacgta gaaactgcgc acggtcatca agcggcgagt
     1981 ctcctcatag atcgtctcca gcactgtcgg cagatcgaga ctgttcgcga ctgcttgtcc
     2041 caccgcgctt aaagaagaga tctcttggac gcgtttttcc aatcgggtgc gggcatcact
     2101 cattgagtgg atgatcgcca tgatcgtaat caagaaaacg cagaaggcgc cgaagcccgg
     2161 aatcgaaata ttagcggcgg tataagcgat gattgtggcg gtccataatg gtgcaagttc
     2221 caatatcgcc accgagcgcc catgctgtcg gatatattca cgtaccgatt ggccgcctgc
     2281 caacttgaag agtaagccga atagaaaata attaatgagc gcgtagccgg taaacaagcc
     2341 gaggattggc acaataagtt gattgaaggt ggcattggaa gcgggagtga gattggcttg
     2401 caatagcggg ctgggcgcca cgccccccaa ggccagatac aattttccgc cgccgatcac
     2461 gctcaagcca tagatcgcaa tattgctgaa gaggaccacg aagattcgcc agtgcgtgcg
     2521 ctgtctggtc ttgaatggcc gagggaagag gacgcgcaat acttcactga gaacgatgcc
     2581 ggtgacggtg acaatcactg ccggaactaa accgagcgag aggaacgctg cgacgataat
     2641 taaactggca aagttggcgt cgctcgattc aaacgaaatg cctaaattgg cggcaatggc
     2701 caaggtgacg atccaaatca tatacaacca tggggagaga acgggctgcg gggcttcagt
     2761 gacgatccgc acgctgttga taatggtcgc tattcccaag atgatggcga cgggctcatc
     2821 cagcgatcga gttttgcggg gagagggtag ctcagtcatt tacggattga gttgaaagat
     2881 ccattgaccg atgcaatcga gtccgatatt ttgcgtggca tgcgtagtct aacacagaag
     2941 agaagcagcg acaaaccgcc tagcggcgca tggtataatc ccgcgcgagt tgcttatggc
     3001 aaatggttta tcgcgtatcg catatcgcat caggcttatg aatcgacatt acttgccgct
     3061 catccgtcat tcctcacacc tgcacagcaa cgcacgcagt gcggtgcaag tgttactcat
     3121 tactcgttac gcattactca gcatgtttct ggccgcatgt ggtggtcaaa ttatcagcgg
     3181 gccgacacct acgccgcact cgttggctgt tattctaccc tcgcccacct cgatctcgac
     3241 tttatcgccg cgaaagttga caccggatcc cacggaaacg ccagccccaa caccgacgcc
     3301 cgtcatctac attgttgagc caggcgatac gctacttggg attgcattgc aatataatgt
     3361 cacggtcgat gaactacaag cggtcaacgg cgtattgtcc cccgaaacac tgcaaattgg
     3421 tcagaagttg atcattccag tcggcggctt ctcagtgccc tcttcgagcg gcggcattca
     3481 attgccttca ccgacgcccg ttggaattga agtgcagcac tcagcgctgt atcaaacgct
     3541 ggttggcagc agctgggtgt tgggtgaagt cttcaatccc ggttcaatag ctttggagaa
     3601 tgttgaagtg cgggttgcct tgttagatgc cactggtcaa gaagttgcgt tcgattctga
     3661 atttgtcgcg ctagaagcca ttccacccgg cggtcgatca ccgtttgggg tgttgtttgc
     3721 caatccgccc gcggtcgtcg tcgcttttca agcgaccgct atccgcgccg agccttcgca
     3781 aaatgtggct gcgcgctatg tgcagctgca cgtgactgac ccgcagccca aacttgttga
     3841 atcgatctat catctcaccg gatcgatcgt taatggcacg tcgagcaacg ccgtcgaagt
     3901 acacgttgtt gcgacaatct atgatgccga tcggcatgta gtcggttacc ggcgatcgat
     3961 ctggggtgac ggatcgatcg gtgctggagt cagtgcgccg ttcgacatca gcgtgtcgcc
     4021 tgattcttcg gccaaaccgg tggccgatta ttcgatcgta gcccaggggc gagtgggaca
     4081 gtagccaatg aggactgatc aattagcaat taacaatccc actccgtatg cgcttcgggg
     4141 actacgtgat caatgccgag tgatcagatg tctacaaatc taactgacct caatccgctt
     4201 ccgcgtcaca ctacgcccgg ttggcaacgc ctactccgat tctttgcgcg aatgatcatg
     4261 ctgcctgtcg cgcggatcga cgtgcagggc atggagcatg tgccgccaga cgccttcatg
     4321 ggcgtcacca accatatgag ttcgttcgat acgttagcgc tgatcgctat cggcccgatc
     4381 cggcgcatcg cgctctttgc ggccattgaa catcgatcgg atttcattgc gggctgggcg
     4441 ttgaatcaac tgcgcgtgat ttggctgcgg cgcggtgaag ccgatcgaga agcgatcaag
     4501 atcgcgctgg atgaaatcaa aagcggctcg gcgtttggga tcgcgattga ggggacacgc
     4561 agcaagactg gtgccttgct cgaaggcaag acaggcgcag cctatttagc gacgcgcgcg
     4621 aatattccga tcttgccggc cgtcatttgg ggcacggaaa aaatcaagca taacctgcga
     4681 aagttcaaac gcacgacagt tcacattcga atcggcgagc cgtttcattt gccggaaggc
     4741 cgcgccggaa ccgaacagtt gcagcagtat accaatcaga tcatgctcaa gatggcgtcg
     4801 atgctgccga gtgaatattg gggagtgtat gcggaaagga tgaaggagca attggcaatg
     4861 aacaatgatc aattagcaat cccgctccgc taacgcttcg gggactgcgt gacaaatgaa
     4921 caatgaccaa cgactagcct acttcccaat ccctgatccc tgatcaacta aattcgccaa
     4981 ccctgcccat tcctctaacg tcaacgtctc ggcccttctc ttcggatcga tgttccccgc
     5041 ttgtaacagc cgatcgattt cttcgcgcgg tttccccaat ccaccgatga gcgagttgtg
     5101 aatttgtttc cgcttttgcg agaagcctgc ctttaccacg ctaaagaaag tatctacgct
     5161 tttaacttca actcgcggct tcgcatccag atcgatccgc acgaccgccg aatcgacttt
     5221 aggcgcggga taaaacgcgc cggcctgaat gttcgtcacg atacgcggtg atccgtagaa
     5281 ctgcacgctg accgccagta ggttcatgtc gtcaggctga gcgatgattc gttccgcgac
     5341 ttcttgttgt atggtgagca ctaagaggct aggtgtgatc gaggcttcga gcagatggcg
     5401 gatgatgccc gaagtgatgt aatacggaat gttggcaacc actttgtaag tagacgcttc
     5461 tcgcagtaaa gtcgctggat cgatcttcag gatgtcgccc tgcacaatct ccacgttcga
     5521 tcgatcggct aattgtgaat gcagcaccgg gatcaggttg ccatcgatct cgacggcgat
     5581 cacgcgcttg gcaattgcgg ctaaatgttg agtcagactg ccgaggccgg ggccgatctc
     5641 cagcaccaca tcatcggcag taacatcggc cgcacggacg atcttatcga gcgcgggatt
     5701 atcgatcaaa aaattttggc cgaggctttt cttgggacgc agcccaaatt gcttgagcag
     5761 ttgacggggt tcgagcatga tgagtgaaaa gtggtaagtg attgcgtgat gtgtgacact
     5821 tgcaacccac tgcgtgcgtt gcagtgcagg tgtacgtgag taaaaacttc aatcatgcat
     5881 cacatcagcg gccagtctcc ggcggccaat ccggcaggac gtacgtgatc cgttcgggtg
     5941 acggagcagg cgcgagcagg tagacatcga cccagcgata ccacaaaacc aggttgttat
     6001 cgtcgtaacc aagatcaagc cgtttgcctt gaatcgcgct gccggtatcc agcgcttgac
     6061 cgaaaccata gccgggcaca tacatattcg taaacaacaa gatcacactt ttatcaactg
     6121 cgatgacacc tttgcgcatt tgctgtccag tgacggtgcg gccataatac tttgcggtgc
     6181 tgggtgtgcc ggctgtcgag gcgctgtacg atgtggcgtg cgcgcgcagg tgccgccaat
     6241 attgaatcgg gccgtcgggc gtgtcgagcg tgcgcacgac gatctgcgtg ccatagcgat
     6301 acactttgct ctcgggcggc tgcaacacaa attcatcggc cacattgcgc gcgacttcca
     6361 gtccgttttc aaagcgtacg cgtatgcgtt tctgcttgac gccgctctgc ccggccgtca
     6421 cactgctggt atcaagttcc atgctgtcat ccgccaccgt cagagtattg aacggaatcg
     6481 gttcctgctg gatcaaaaac tcttcgcgca cgcgcacgac attgacaact gcgcctgatt
     6541 caactgacgc atcaactgtg ggttgcgtgt aatcttcacc caacaacgtc acgcccagat
     6601 cattcaacac ttcgcccacg gtcgaccggc gtgtgcgcgt tttcaacaac tgcccatcga
     6661 cttggacatt gatcggcatc gaaggctcga tcacaacctg tagattcgat tgaagcggcg
     6721 tggcgagatc gggcgtgatc cgatcggcca gataaacctg cacgttcgct tcggccaacg
     6781 cttgcccgat cgtatcggcg gcgctttcaa atttttgtgc gttagtgccg atcttgatct
     6841 cgattggcac cgcgcgcttg atctcgatcg tcacacgctt gagatcgatc acgctcaaga
     6901 tcgatgggca gacgcgcgtc attcgcacca atcaacgctc gatcgaaaat attttgcgcg
     6961 atgcggatat aacgatccag ccggaagacc tcgtcgtgcc cgattattcc gccacactcg
     7021 acgatcgaca gtccgcgatc acggtgcagc acgctcgatc gatcgcgatc gacgtggacg
     7081 gccagacgaa acaatttcga tcgcgcgcga cggccttgga tgaactcttc aagcaggcca
     7141 atattgaggt cggcgcgcac gatcgaatca gtgtgaatgg ccgcgtcg
//
LOCUS       DASRTJ010000708         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_297964,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000708 DASRTJ010000000
VERSION     DASRTJ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297964"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFF70_14360"
     CDS             <1..1184
                     /locus_tag="VFF70_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZY45919.1"
                     /translation="MLLMLAVVFWITITFANIPSQWIAAALFWIEDQAASLFTSLGIP
                     AWMTGFIWHGTYRGLAWVVAVMLPPMAIFFPMFTILEDLGYLPRVAFNLDRLFKKAGA
                     HGKQSLTMAMGFGCNAAGVIATRIIDSPRERLIAILTNNFVPCNGRFPTLIMLATVFV
                     AASFSPALASLAAAGSVVLVVLIGVLATLVVSWALSRTILKGEASAFTLELPPYRRPN
                     VGRILYTSLIDRTLFVLWRAMKTAAPAGAVIWVLANITVGDASLATHIANLLSPLGFA
                     IGLDGVIMLAYIIAIPANEIVVPTMLMAYTGAGMMIGPEGFADLRTLLMGAHGWTMLT
                     VVNLMLFSLLHNPCATTILTMWKETRSKKWTTIGALMPLVIAFAVTFVTASVARLLGW
                     V"
     gene            1219..1380
                     /locus_tag="VFF70_14365"
     CDS             1219..1380
                     /locus_tag="VFF70_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45920.1"
                     /translation="MHVKVNVWARRLIAMIPLIKFTRNRKIMGDFANSHFVNGLAYLV
                     AAIILSLNA"
     gene            1597..>2465
                     /locus_tag="VFF70_14370"
     CDS             1597..>2465
                     /locus_tag="VFF70_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZY45921.1"
                     /translation="MNTISRRTFLKAGSASVLGAASLISIVNQPAKDQPTPPAHADHS
                     AGHGGNLMVGDVDPNNINHFDPTAMLTDWDYGKVSQLPNGQTLREYEIFAGDKEIEIA
                     PGVMFSAWTYNGRVPGPTLRCTEGDHIRIHFTNGSSHPHSMHFHGIHSGAMDGIEPVA
                     VGGTFTYEFDAEPFGLHLYHCHVLPLKHHIHKGLYGGFIIDPKQGRLPAHELFMMMNG
                     FDTDFDNENDVYAVNTVGFHFQRHPIQIKLNELVRVYLVNILEFDLINGFHLHANFFD
                     VYRTGTRLTTNEYT"
BASE COUNT          510 a          711 c          704 g          540 t
ORIGIN      
        1 tcatgttgtt gatgctggcg gtggtgttct ggatcaccat tacgttcgcc aacataccat
       61 cgcaatggat cgccgcagcc ttgttctgga tcgaggatca agctgccagc ctctttacca
      121 gcctgggcat tcccgcttgg atgacgggct ttatctggca cggcacctat cgcgggctgg
      181 cctgggtcgt ggcggtgatg ctgccgccga tggcgatctt cttcccgatg ttcacaattc
      241 tggaagacct gggctatctg ccgcgcgtgg cctttaatct cgatcgactg ttcaagaagg
      301 ccggagcgca cggcaaacaa tcgctgacga tggcgatggg cttcggctgc aacgcggcgg
      361 gcgtcatcgc cacacgcatc atcgatagcc cgcgcgagcg cttgatcgcg atcttgacaa
      421 acaacttcgt gccctgcaat ggccggtttc ccacgttgat catgctggcg acggtctttg
      481 tcgcggcgag cttttcgccg gcgctcgctt cgctggccgc ggccggatcg gtcgtgctgg
      541 tggtgctgat cggcgtgctg gcgacgctgg tcgtatcgtg ggcattgtcg cgcacaatct
      601 tgaaaggcga agcgtcggcc tttacgctgg agctgccacc ctatcgacga cctaacgtcg
      661 ggcgcatcct gtacacctca ctgatcgatc gaacgctgtt cgtgttgtgg cgagcgatga
      721 agaccgccgc gccagccggc gctgtgatct gggtgctggc caatatcacg gtcggcgatg
      781 ccagtctggc gacgcatatc gccaacttac taagtccgct gggtttcgcg atcggcctcg
      841 acggtgtgat catgctggcc tacatcattg cgatcccggc caacgaaatc gtcgtgccga
      901 ccatgttgat ggcctacacc ggcgcgggca tgatgatcgg gccggagggc tttgccgatc
      961 tgcgcacgct gctgatgggc gcgcacggct ggacgatgct gacagtcgtt aacttgatgt
     1021 tattcagcct gctgcacaat ccgtgcgcca caaccatctt gacgatgtgg aaggaaacgc
     1081 gcagcaagaa atggacgact atcggcgcgc tgatgccgct ggtcattgcg ttcgcagtga
     1141 cgttcgtgac cgccagcgtg gcgcgactac tgggctgggt ttagaggacg ccgatcaaag
     1201 tgatcatgga agggattctt gcacgtaaag gtcaacgtgt gggcgcgccg cttgatcgcc
     1261 atgattccgt tgatcaagtt cacacgcaat cgaaagatca tgggcgactt cgccaacagt
     1321 cacttcgtta acgggttggc ttatctggtg gccgcgatta ttttaagttt gaatgcctga
     1381 ctcgtggtgc aaattttgag cgggggaatt tagtgagacc aatacggaaa gtgttgtgcc
     1441 ttggctcgaa gcggagcgcc agctccgcgt aacccgccgc cagagctggc gctctagcgg
     1501 gagcatgaac atcaagcaac accgctcaag gcccgcgtcc gcgcggacgc ggttcgtaga
     1561 gcaacagtta catttacaag ccacagggag aaaaaagtga acaccatttc aagacggaca
     1621 tttctgaaag caggctcggc cagtgtgttg ggggcggcca gcctgatctc aatcgtcaat
     1681 cagcccgcca aagatcaacc gacaccgcca gcgcatgccg atcattccgc cgggcatggc
     1741 ggcaacctca tggtcggcga cgtcgatccg aataacatca accacttcga tccgacggcg
     1801 atgctaaccg attgggatta cggtaaggtc agccaattac ccaacggaca aacgctgcgc
     1861 gagtacgaaa tcttcgcggg ggataaagaa atagagattg cgcctggcgt catgttttcc
     1921 gcctggacat acaacggacg tgtgcccggc cccacactgc gctgcaccga aggcgatcac
     1981 atccgcattc atttcacgaa tggatcgtcg catccgcact cgatgcattt tcatggcatt
     2041 cactccggtg ccatggatgg gattgagccg gtcgccgtcg gcggaacgtt cacttacgag
     2101 ttcgacgcag aaccgttcgg cttgcatttg tatcactgcc acgttttgcc gctcaagcat
     2161 cacatccaca aagggctcta cggcggcttc atcatcgatc cgaaacaagg ccgcctgccc
     2221 gcgcatgagc tgttcatgat gatgaacggc ttcgacaccg atttcgataa cgagaatgac
     2281 gtgtacgccg tcaacaccgt cggctttcat tttcagcggc atccgatcca aattaaattg
     2341 aatgaactgg tgcgcgtgta tctggtcaac atcctcgaat tcgatctgat caacggcttc
     2401 cacttgcacg ccaacttctt cgacgtctat cgcaccggca cccgcctgac caccaatgaa
     2461 tacac
//
LOCUS       DASRTJ010000709         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_213210,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000709 DASRTJ010000000
VERSION     DASRTJ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213210"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFF70_14375"
     CDS             <1..888
                     /locus_tag="VFF70_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45922.1"
                     /translation="YEAKGVSNEQALGDIPLSRRLDASDPYRTLDGYQWQTISTTAAG
                     PNLNLEAFSVAPQMPKSLDQEDVTMRWRKTGSIDTAAPHIRLIQNDRVWVDLSSNLFE
                     RDYPIGQWLNGETVIEHRTLTYPPIAGQARLQIGQGESWTTLITLTLNTSVLSFTAPI
                     MQYPQSASYNHFTDLVGYDLSADSIALYRPLELTIYWRAMNTEPITTSYTIFTQLIAA
                     DGHLVAQQDGLPSPIVNAWVNGQVIADRHTLKVVDPAYHGPATLIVGWYNSATIERVK
                     TITGADHIILQSPLTVNDQ"
     gene            892..3138
                     /locus_tag="VFF70_14380"
     CDS             892..3138
                     /locus_tag="VFF70_14380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY45923.1"
                     /translation="MTNDKVDYATRNMLHVSRVTRHSLLIILSTFVVLATLYSITTPI
                     FEGPDEIWHYAFADHLANGGGLPVFEVNQSATFLRNGAHPPLYYWLVAAVIAPIDRSD
                     FPAQFHFNLASPKITPGGQSDRPNLFIHTAHEDFPFHNTTLAVHLARLISIVLGVITI
                     VGVWKVARQLLPDRDRLAIAATALAAFVPQFVYGSATVNNDALAAAATTWTVFALARL
                     MEERSARWSILSGVLLGVTLLSKIGMVAILPLPLIGLLVSNNHEVSKSPSYKKNLRVF
                     VSSWLKSIQWKQIVVSGFIIYIAAFAIAGWWYVRNWNLYGDPLAWKQWQALTGVGRVP
                     PSIGDFMHDMIGLFGTFWADFSLRVDHTWVWVFSLLTLIAVAGLIRRMIRHAWPSIDW
                     RGLVIALVWFGLLLSSAVRYSFSINDIHGRLLYPALAAIGVVLVLGLSGWNEIIGRRL
                     IFGAIGGLIVVNAIVPVGLIQLAYAPPVVAALPNGVTKTSIKFDDIKLIGYRLKNEQI
                     KSGESVAVATYWQSGGPITEPLPILHGITSLATRGGIIDRIDYTLGTDAYPRWAWRPN
                     EIVETDFLLRTESSGTYVANVLLGVRGESAQLITSSAGDSINLGQVVVKDNRTCDVDH
                     VSEVPFGGSIKLIGYRVYQKTMIGLPSSVILCWQSIKPTAIDYTVFVHVIDNQGGLIT
                     SDAQPLNGNYPTSAWVADDQIEDSHPLPAVIDLVIKRVSVGLYRLDTGERLTIDGTNE
                     TEFVLTTP"
BASE COUNT          719 a          781 c          860 g          871 t
ORIGIN      
        1 tatgaagcga agggtgtatc gaacgagcag gcactcggtg acattccatt gtcacggcga
       61 ttagacgctt ccgatcctta tcgcacgctc gacggttatc aatggcagac catttcaacc
      121 acagctgctg gacccaatct gaatctcgag gcattcagtg tagctcctca aatgccaaag
      181 tcacttgatc aagaggacgt gacgatgcgt tggcgtaaaa ctggatcgat cgacactgct
      241 gcaccgcaca ttcggttgat ccagaacgat cgggtttggg tagatctcag ctcgaatctc
      301 ttcgagcgtg attatccgat cgggcaatgg ttgaatggcg aaaccgtgat cgagcatcga
      361 acgttaactt atccgccgat cgccggtcaa gcgcggttgc agatcggaca gggcgagagc
      421 tggacgactt tgatcacact cacgttgaat acatcggtgc tgagttttac cgcgccgata
      481 atgcagtatc cacagtcggc atcctataat catttcaccg acttggtggg ttacgatctg
      541 tcagcggatt cgatcgcact ttatcgaccc ttagaattga cgatctattg gcgtgcgatg
      601 aataccgagc ctattaccac ttcttatact atcttcacac aattgatcgc agcggatggt
      661 cacctggtcg cgcagcaaga cggcttgcct agcccgatag ttaatgcgtg ggtgaatggt
      721 caagtcatcg ccgatcgaca tacgctgaaa gttgtcgatc ctgcgtacca tggccccgcg
      781 acgctgatcg tcggatggta taattctgcg acgattgagc gagttaagac gatcactggc
      841 gctgatcata ttattttgca gtcgccgctt acggttaatg accaatgact aatgacaaat
      901 gacaaagtgg attacgcaac acgcaatatg ctgcacgtgt cacgcgtcac tcgtcactcg
      961 ttactcatta tcttgtcaac gttcgttgtt ttggcgacgc tctactccat caccacaccc
     1021 atctttgaag gcccggatga aatttggcat tatgcgtttg ctgatcattt agccaatggt
     1081 ggcgggctgc cggtcttcga ggtgaatcaa tctgccacct ttttgcgcaa cggcgcgcat
     1141 ccaccgttgt attattggct ggtcgcggcc gtcattgcgc cgatcgatcg cagcgacttt
     1201 ccagctcagt ttcacttcaa tttggcgagt cccaagatta cgcccggcgg gcaaagcgat
     1261 cggccgaact tatttattca cactgcccac gaagattttc catttcacaa cacgacgctg
     1321 gcggttcatc tggcgcgctt gatctcgatt gtgttgggtg tgatcacaat cgtcggcgta
     1381 tggaaagtgg cgcgtcaact gttacccgat cgtgatcgac tggcgatcgc ggcgacggcg
     1441 ctagctgcat tcgtgccgca gtttgtttac ggctcggcga cggtcaacaa cgatgcgctg
     1501 gcggcggcgg ccacaacctg gacagtgttt gccctggcac gattgatgga ggaacgatcg
     1561 gctcgctggt cgatcctcag cggtgtattg ctgggtgtca ccttgttatc gaagatcggc
     1621 atggtggcga tcttgccgct gccgctgatt gggttattgg tgtcgaataa ccacgaagtc
     1681 tctaagtcac caagttacaa aaaaaatctt cgtgtctttg tgtcttcgtg gttaaaatcg
     1741 atccaatgga aacagatcgt tgttagtggt ttcatcatct acatcgccgc atttgcgatt
     1801 gcaggttggt ggtacgtacg caattggaat ttgtacggcg atccgctggc gtggaagcaa
     1861 tggcaggcgt taacgggtgt cggtcgtgtc ccgccatcga tcggtgattt catgcatgac
     1921 atgatcggtc tgttcggcac gttctgggca gacttttcac tacgtgttga tcacacttgg
     1981 gtgtgggttt ttagtctgtt gacgttgatc gctgttgctg gcttgatccg ccgtatgatt
     2041 cgacacgcct ggccgtcgat cgattggcgt ggattggtta ttgcgttggt atggtttgga
     2101 ttgttgctct cgtcggccgt gcgatattct ttttcaatca acgacattca cggccgattg
     2161 ttgtatccag ctctggctgc gattggcgtt gtgttggtct tgggcttgtc aggttggaat
     2221 gagatcatcg ggcgccgatt aatcttcggg gcgatcggcg gattaatcgt agttaatgcg
     2281 atcgttcctg ttggattgat tcagttggct tacgcgccgc ccgttgtcgc ggcgctgcca
     2341 aatggagtga caaaaacttc aattaagttt gacgatatca aattgattgg ttatcggttg
     2401 aagaacgaac agatcaaaag cggtgagtcg gttgctgtag caacgtattg gcaatctggc
     2461 ggccccatca ctgagccttt gcctatcctg cacggtataa catctctcgc aacgcgtggc
     2521 ggaataatcg atcgcattga ttacacacta ggcacagatg cctatccgcg ttgggcatgg
     2581 cggccaaacg aaattgtcga aactgatttt ctgttacgaa ctgagtctag tggaacatat
     2641 gtagcgaatg ttttgttagg tgttcgaggt gagtctgccc agttaattac ttcgagtgcg
     2701 ggcgactcca tcaatctcgg gcaggtggtt gtgaaggata atcgaacgtg cgatgtcgat
     2761 cacgtttccg aagtgccctt tggaggttct atcaagttga tcggatatcg cgtttatcaa
     2821 aagactatga tcggcttacc atccagcgtg atcttgtgct ggcaatcaat caagccgaca
     2881 gccatcgact atactgtgtt tgtgcatgta atcgataatc agggtggtct catcacttcc
     2941 gatgctcagc cgcttaatgg caactatccg acatcagcgt gggtagccga tgatcaaatt
     3001 gaggattcac atccgttacc ggcggttatt gatttagtta tcaagcgagt ttcagtcggc
     3061 ctttatcgcc tcgatactgg cgaacgctta accatcgacg gcacaaacga aaccgaattt
     3121 gttttaacga ctccataatg cctcatcaaa acgcagacaa caacaagcag aaattgaaca
     3181 aagtcatcgt gagcggcggc gggccagcag gcttgaccgc cgcttacgaa t
//
LOCUS       DASRTJ010000710         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_298441,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000710 DASRTJ010000000
VERSION     DASRTJ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298441"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="rlmN"
                     /locus_tag="VFF70_14385"
     CDS             complement(<1..1019)
                     /gene="rlmN"
                     /locus_tag="VFF70_14385"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZY45924.1"
                     /translation="MTKTQSSLFDLNFDQLQTLMSEWGESSFRAKQIWDWLYVRLADR
                     YGVMSNIAAPLKERLKIEFPFNRLTPKIDLLSSDHWTRKILFTLPDGAQIETVLMDYD
                     SRHTVCVSTQAGCAMGCTFCATGQGGFQRNLSSGEIVEQVLFFDRELRQSENNANRKV
                     QDVSQHRLTNLVFMGMGEPFANYTESMDAVQRLSDPTGYNFGARRITVSTVGLVPMIE
                     RFTQETSQVNLAVSLHAATDELRESMLPITKRYSLSMLMAACRAYTEQTHRRISFEWA
                     LIEGINDTPEQARLLGRLIKGMLCHVNMIPLNPTRDFAGQASTRDRIAAFRSVLDLYK
                     IPNTLR"
     gene            complement(1023..1835)
                     /locus_tag="VFF70_14390"
     CDS             complement(1023..1835)
                     /locus_tag="VFF70_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZY45925.1"
                     /translation="MTLPQSPCPLPRCLITDMDGVLWRGKQPMPGLAEFFQFLRDRSV
                     RFMCATNNSSSTPEQIADRLHAFGANVTPDEIVTSSIATADYLQTILPTGSKVYIIGM
                     DGVREAITHAGFVIAEDRAAAVVVGIDWNLTYNQLKRATQLIRGGAKFIGTNSDASFP
                     SPDGIIPGAGSLLAAIETATSVKPIVIGKPEVVLYEMALKRMGASIEETVALGDRLET
                     DILGAVRMNLKSILVLSGVTTAEELAASDYQPDWVFRDISDLVMHWSRTNDK"
     gene            complement(1838..>2091)
                     /locus_tag="VFF70_14395"
     CDS             complement(1838..>2091)
                     /locus_tag="VFF70_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45926.1"
                     /translation="LADTYRSTGKPIVVTSVKGKRTTPTLFDRSLFAGIAMVKGDRGP
                     RELIDADASRVVKVEIDAHSAVDIDTPDDYHWLFNASEL"
BASE COUNT          461 a          572 c          571 g          487 t
ORIGIN      
        1 cgtaatgtgt tggggatttt gtacagatcc aagaccgatc ggaatgcggc aatccgatcg
       61 cgcgtggaag cctgcccggc aaaatcgcgc gtgggattga gcgggatcat attcacgtgg
      121 cacaacatgc ctttgatcaa acggccaagc agccgcgcct gttcgggcgt atcgttgatg
      181 ccttcgatca gcgcccactc gaaactaatg cgtcgatgag tctgctcggt gtacgcgcgg
      241 caggccgcca tcaacatact caacgaataa cgcttggtga tcggcagcat cgattcacgc
      301 agttcgtcag tggccgcgtg cagtgagacc gcgagattga cttgggatgt ttcttgtgtg
      361 aagcgttcga tcatcggcac gagaccgacg gtgctgacgg tgatccgtcg cgcgccaaaa
      421 ttgtaacccg tcggatcgct caagcgttgc actgcatcca tcgactccgt gtaattggcg
      481 aatggctcgc ccatgcccat gaaaactaga ttggtcagtc gatgctggct tacatcctgc
      541 accttgcggt ttgcgttatt ctcgctctgc cttaactctc gatcgaagaa caacacctgc
      601 tcgacgattt cgcctgacga caaatttctt tgaaaaccac cttgacctgt cgcgcagaac
      661 gtgcagccca tcgcgcaccc tgcctgcgtc gagacacaaa ccgtgtggcg tgagtcgtaa
      721 tccatcaaca ccgtttcgat ctgcgcgcca tccggcagcg tgaataaaat cttgcgcgtc
      781 caatgatcgc tggacagcag atcgatcttc ggcgtcagac ggttgaacgg aaattcgatc
      841 ttcaatcgct ctttgagcgg cgccgcgata ttactcatta ccccgtagcg atcggccaag
      901 cggacgtaca gccaatccca aatctgcttg gcgcggaacg acgactcgcc ccactcgctc
      961 atcagcgttt ggagttgatc gaagtttaga tcaaagagtg acgattgagt ttttgtcatt
     1021 ggtcatttgt cattggtcct tgaccaatgc atcactaaat cgcttatatc ccggaagacc
     1081 caatcgggtt gatagtcgct cgccgctaat tcttccgctg tggtcacgcc gcttagcacc
     1141 aagatcgatt tcaagttcat acgcactgcg ccgagaatat cggtctccaa tcgatcgccg
     1201 agcgcgacag tttcctcgat cgacgcgccc atgcgtttga gcgccatttc atacagcaca
     1261 acttcgggtt tgccaattac gattggcttc acactcgtcg cggtttcgat cgcggctaac
     1321 aatgagcctg caccgggaat gatcccgtcg ggcgaaggaa aacttgcgtc cgaattagtg
     1381 ccgatgaatt ttgcgccgcc gcgaatcaat tgtgtggcgc gcttgagctg attatacgtg
     1441 agattccaat cgatgccgac cacgacggcg gccgcgcggt cttcggcgat aacgaagccc
     1501 gcatgagtga tcgcctcgcg tacgccatcc atgccgatga tatatacttt tgatccggtt
     1561 ggcagaattg tttgcaaata atcagctgtg gcgatggacg atgtaacgat ctcgtcgggc
     1621 gtaacattcg cgccgaatgc atgcaaccga tcggcaattt gctcaggtgt agaagacgag
     1681 ttgttcgttg cgcacatgaa ccgcacggat cgatcgcgca ggaattgaaa gaattcggcg
     1741 aggccgggca tcggttgttt gccgcgccac agcacaccgt ccatgtcggt gataaggcag
     1801 cgaggcaagg ggcagggtga ttgcggaagt gtcatagtta aagttcactc gcgttgaaaa
     1861 gccaatggta atcgtcaggg gtgtcgatgt ccaccgcgct gtgtgcatcg atttctactt
     1921 ttacgacacg tgacgcgtct gcatctatca actcgcgcgg accgcgatcg cctttaacca
     1981 tggcgatgcc ggcaaacagc gatcgatcga acaaggtggg cgtggtgcgt ttgcccttca
     2041 cgctggtgac gacgatcggt ttgcccgtcg aacgataagt gtccgcgagg a
//
LOCUS       DASRTJ010000711         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_298473,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000711 DASRTJ010000000
VERSION     DASRTJ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298473"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFF70_14400"
     CDS             <1..898
                     /locus_tag="VFF70_14400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45927.1"
                     /translation="LCAALQPDAILSIQRYANIAILQSRLLTRRRVPVIVNEQNRIGF
                     EFQQFGGGRFKRAMLKWLYPHANAITVISHGIAQELAQDYSIDPRRVHVIYNPIDLAA
                     LQDRSHSAPPHPWLAENDPPTLIAAGRLVPQKGFDLLLHALTRIKAERFVRLIVLGEG
                     PDRSMLEKLRDDLGLRAVVEFPGFQSNIAAFYSRAATFVLASRYEGFGNVLVEAMACG
                     TPVVSFRCPSGPDEIIEHEQSGLLVPPEDVAALSAAIMRVLGDRALADLLRRGGLARA
                     KTFDAPIIAAQYADLIEALCAS"
     gene            886..2013
                     /locus_tag="VFF70_14405"
     CDS             886..2013
                     /locus_tag="VFF70_14405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZY45928.1"
                     /translation="MRILIATDIFPPDIGGPATYVPIIAAELAQRGHAVRVLTYSQTD
                     QVDTHYPFKVERILLRGSRVARLLRTFRRIASNVHWAEVVYLNGLLIETSIVNFILRK
                     PAAAKVVGDITWERARDKGWIDAEFEDFQNKKYGWRIELRRGLRNWALRRARIIIVPS
                     AYLKRIVSHWPINLDRVQIVYNSFEPKMPAAASIDLPLKTHYRIITSCRLTAWKGVDG
                     LIEAIAALPDTGLVIVGDGPERSRLIDLARRLKVTDRVYFVGQVPADQVAGYLRACNL
                     FVLNSRYEGLPHGILEALATGLPVIAADAGGTSEIVQDRVNGRLVPVLDQAALQAAIR
                     DVLDRPELRQAWISASQKTLERFSRQVMIEHTVEILEAALS"
     gene            2010..>2395
                     /locus_tag="VFF70_14410"
     CDS             2010..>2395
                     /locus_tag="VFF70_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45929.1"
                     /translation="MIDKIPVALLTISIDPDLAKPAVLSPGDAYDRQLKYAGILAEYY
                     AITYIKQASADSAPIQVTDNFTVYPLHAQNLLWFVRAAVDFGSHLCRTKKIDAISTQD
                     PFFTALIGYRLKKKFGLPLSIQSVAD"
BASE COUNT          520 a          667 c          654 g          554 t
ORIGIN      
        1 cttatgtgct gcgctgcaac ccgacgcgat cctctcgatc cagcgttacg ccaacatcgc
       61 catcctgcaa agccgcctgc taactcgccg cagagttcct gttatcgtca acgaacaaaa
      121 tcggatcgga tttgagttcc agcaatttgg cggcggtcga ttcaaacggg cgatgcttaa
      181 gtggctgtat ccacacgcca atgcgatcac ggtgatctcg cacggcatcg cccaggaatt
      241 agcgcaagac tattcaatcg atcccagacg ggtgcatgtc atctacaacc cgatcgatct
      301 cgccgcgctt caagatcgat cgcattctgc gccaccgcat ccgtggctgg cagaaaacga
      361 tccgccgaca ttgatcgccg ccggtcgatt agtgccgcaa aagggctttg atttattgct
      421 gcacgcgctg acccgcatta aagccgagcg gtttgttcgc ctgatcgttt tgggtgaagg
      481 gccggatcga tcgatgctgg aaaaactaag agacgatctc ggcttgcggg ctgtggttga
      541 gtttcccggt tttcaatcga acatcgctgc gttttattca cgcgccgcca cttttgtctt
      601 agcgtcacgc tatgaaggct ttggcaacgt gctcgtcgaa gcgatggcct gcggtacgcc
      661 ggttgtctcc ttccgatgtc cgtcggggcc ggatgagatc atcgagcacg aacaatccgg
      721 cttattggtt ccgccggagg acgtcgccgc tttaagtgcc gcgatcatgc gcgttctggg
      781 cgatcgagcc ttagccgatc tgctgcgtcg cggcggactc gctcgagcca aaactttcga
      841 tgcgccgatc atcgccgcgc aatacgccga tctgatcgaa gccctatgcg catcttgatc
      901 gccaccgata tttttccacc ggacattggc ggtccggcca cttacgtacc gatcatcgcc
      961 gccgaattgg ctcaacgcgg acatgccgtg cgcgtgttaa cttacagcca gactgaccag
     1021 gttgataccc actatccgtt caaggtcgaa cgcatcctct tgcgcggctc acgcgtggca
     1081 cgtttactgc ggacgttcag gcggatcgct tcgaatgtgc attgggcgga agtcgtctac
     1141 ctcaacggac tcttgattga aacatcgatc gtcaatttca ttttgagaaa accggctgcc
     1201 gccaaagtag tcggcgacat cacctgggaa cgcgcgcggg acaaaggctg gattgacgcg
     1261 gagtttgaag attttcaaaa taaaaagtat ggctggcgga ttgagctgcg gcgcggcttg
     1321 cgcaattggg ctttgcgtcg agcgcgcatc atcatcgtac ccagcgccta cttgaagagg
     1381 atagtcagcc attggccgat caatctcgat cgggtacaga ttgtttataa ttcgttcgag
     1441 ccgaaaatgc cggcagcagc atcgatcgat ttgcctctaa agacccacta tcgaataatc
     1501 accagctgcc gtctaacggc ctggaaaggg gtcgatggat tgatcgaagc gatcgccgcg
     1561 ctgcccgata ccggcttagt gatcgtcggc gacgggccgg agcgcagccg cttgatcgat
     1621 ctggcccggc ggctgaaggt gactgatcga gtatacttcg tcggtcaggt gccagccgat
     1681 caagtcgccg gttatttacg cgcatgtaat ttgttcgtgc ttaattcgcg ctatgagggt
     1741 ttgccgcatg gcattttgga agcgctggcc accggactgc cggtgatcgc ggcagatgcc
     1801 ggcggcactt ccgaaatcgt tcaggatcga gtgaatggcc gcctagtccc ggtacttgat
     1861 caagctgcgc tacaggctgc aattcgcgac gtgctcgatc ggcccgagct cagacaagcg
     1921 tggatcagcg cgagccaaaa aactttggag cgcttcagcc ggcaggtaat gatcgagcac
     1981 actgtggaaa tattggaagc ggcgctatcg tgatcgataa gattcccgtc gcgctcctaa
     2041 ccatcagcat cgatcctgat ctggctaaac cggcggtgct cagccctggc gatgcttacg
     2101 atcggcaact caaatatgcg gggattctgg cagaatatta cgccatcact tatatcaaac
     2161 aagcatccgc agactcagcg ccgattcagg tgactgataa tttcacggtc tatccccttc
     2221 acgcgcaaaa tttgttgtgg ttcgtgcgag cagcagtgga cttcggcagc cacttatgcc
     2281 gcacaaaaaa aatcgatgcg atcagcactc aggatccatt ttttacggca ttgatcggtt
     2341 atcgcttaaa aaagaaattt ggcttgccct tgagtattca atccgtcgcc gatca
//
LOCUS       DASRTJ010000712         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_298538,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000712 DASRTJ010000000
VERSION     DASRTJ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298538"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFF70_14415"
     CDS             complement(<1..644)
                     /locus_tag="VFF70_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZY45930.1"
                     /translation="MARVIKHIEHAVKRNEILDAAQKLIYTKGYAQMSIQDILDDLKI
                     SKGAFYHYYTSKQVLLEAVIDRMLQQAEGIVLPIVDDPQLTALEKFQRYFDTVARWKT
                     DRIDLMLALLRIWYTDDNILVRQKVTEQGLKVIMPPLTKIIQQGIQEGTLNTAYPERV
                     CEVIFSMMLAMSDKMAEILLAPPKNTLSQLESTLEVYTDAIEHVLGVSSGSLCL"
     gene            853..1737
                     /locus_tag="VFF70_14420"
     CDS             853..1737
                     /locus_tag="VFF70_14420"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HZY45931.1"
                     /translation="MSDARPIVKWAGGKGQLLDRLRPLFPAKFNRYIEPFAGAGAVFF
                     RLYNLGMIGANPRLRKTPAILNDLNPELMNVYQAARDHIDELIDELARHQAHVDDPDY
                     YYFVRAIDREPNYAQLPVAFRGARTIYLNKTGYNGLYRVNKSGYFNVPFGKHKNPNVL
                     DRSALQAAHRALQHVDLMCSDFAQPLRLAHTSDFIYIDPPYHPVSATSNFVSYTSNGF
                     GWDEQKRLADWVARVIDRGCYVLINNADTPEVRQLYRSAFKSRRIKIHLQSFTTARNI
                     NSRADYRSGSLELTIWNY"
     gene            1829..2167
                     /locus_tag="VFF70_14425"
                     /pseudo
     CDS             1829..2167
                     /locus_tag="VFF70_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
     gene            2298..2370
                     /locus_tag="VFF70_14430"
     tRNA            2298..2370
                     /locus_tag="VFF70_14430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2331..2333,aa:Ile,seq:cat)
     gene            complement(2534..3253)
                     /locus_tag="VFF70_14435"
     CDS             complement(2534..3253)
                     /locus_tag="VFF70_14435"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZY45932.1"
                     /translation="MPDQLKHNAAKQPQKEESSKPHIQIEEHGPYVVTGGVKLTRRIR
                     VRNAQGDAVEWQSGEDYPVETTTYKLCRCGHSDNMPFCDDHHEQEVEWDGSLVADRAP
                     GETRRQLFQGTGMILSDDNSLCAGFEFCDRFGGVWDEINQTADPAISEQLKKQIPLCP
                     SGRLQYLLKRGEKPQEIKYEPMIAAIPDGPVWVLGGIPIEAPDGFTYEARNRQLLCRC
                     GQSKNKPFCDGTHWDIKFKAA"
     gene            complement(3270..3737)
                     /locus_tag="VFF70_14440"
     CDS             complement(3270..3737)
                     /locus_tag="VFF70_14440"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZY45933.1"
                     /translation="MQQLLIDGHNLIAQVPGLALDDPDDEQKLIVMLRKYAARRGAKI
                     VVVFDTGIPGGRSNALSGGGVTAIFAAATHTIADRVLIERINDFPRPADWTVISSDNQ
                     VRAVAFKRKAMLKSAQEFVAIMTAPPKGDDRSVVEEKLSSEEVAEWLKVFKNK"
     gene            complement(3807..>4820)
                     /locus_tag="VFF70_14445"
     CDS             complement(3807..>4820)
                     /locus_tag="VFF70_14445"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZY45934.1"
                     /translation="PHDAVSLLRVINVPPRGIGAKTLSVLDEVSRRGEKSAYQLIHEL
                     KSAGATSPLASSLTPKAKQALVDFAAMIDRFIDSPARGSNIAELIDLILKESAYQRFI
                     DDGTEEGKDRYDNIKELRNVASDYTRAVGDDPLAEFLEDVALVADVDALKDQTDAPTL
                     MTLHSAKGLEFPIVFITGLEEGLLPHARSMEDPDQLEEERRLCYVGITRAKERVLLTY
                     AFRRTMWGSDDVSTPSRFLNDIPKKLLSGSMSFIGSKPKEAAMVRASTWESASAKTRS
                     AEPARAMLFRSGQRVKHGKFGDGIVIESKIDRNDEEVTVAFEKAGIKRLLASFANLTK
                     LPG"
BASE COUNT         1025 a         1378 c         1237 g         1180 t
ORIGIN      
        1 atcagacaca gtgaaccgct cgacacaccc agcacatgct cgatcgcatc ggtgtaaact
       61 tcaagcgtgc tttccagttg agacagagta ttcttcggcg gcgccaataa aatttccgcc
      121 atcttgtccg acatcgccag catcatcgag aaaatcactt cgcagacgcg ctctggataa
      181 gcagtgttca atgtcccttc ttgaataccc tgctgaataa tctttgtcag tggcggcatg
      241 atcaccttca gcccctgctc cgtcactttc tgccggacca aaatgttgtc atcggtatac
      301 cagatgcgca gcagcgccag catcagatcg atccgatccg tcttccagcg agccaccgtg
      361 tcaaagtaac gctggaattt ttccagcgcg gtcaactgcg gatcatcgac aatgggcagc
      421 acaatgcctt cggcctgttg cagcatccga tcgatcacag cttccagcag gacttgtttg
      481 gaagtgtaat agtgatagaa cgcgcctttg gaaatcttca ggtcgtccag aatatcctga
      541 atcgacatct gggcgtagcc tttggtgtag atcaattttt gggcggcgtc gaggatttcg
      601 ttgcgcttga cggcatgttc gatgtgcttg atgacgcggg ccatagtcgg tgcttcctga
      661 taaacagacc gacggtcggt ttatcggagt gagtatatct tatctttttg gctttgtcaa
      721 tatctcgatc gaacatccat ttattctgag gccaaatgtc ctagcaggta agtgacaaac
      781 gtcattcgcg gctgaggcgt cacaggttag tgctgcatgt tataatgcgc tcatcctcat
      841 taggaatcga tcatgtcaga tgcgcgtccc atcgtgaagt gggcgggcgg caaaggccaa
      901 ctgctcgatc ggctgcggcc attgttcccc gccaaattca atcgctacat cgagccgttt
      961 gccggcgcgg gcgcggtctt ctttcgattg tataacctcg gcatgatcgg cgcgaatccg
     1021 cgcttgcgca aaacgcccgc catcctcaac gatctcaatc ccgaactgat gaatgtctat
     1081 caggccgcgc gcgatcacat cgacgagttg atcgatgaac tggcgcggca tcaagcgcac
     1141 gtcgatgatc ccgattacta ttacttcgtc cgcgcgatcg atcgtgaacc caattatgcg
     1201 cagctgcccg tcgcgtttcg aggggcgcgc acgatctatt tgaacaagac cggctacaac
     1261 ggactgtatc gagtgaacaa aagcgggtac ttcaacgtgc catttggcaa acacaaaaat
     1321 cccaacgtgc tggatcgatc ggccttgcaa gcggcccatc gcgcacttca gcacgtcgat
     1381 ctgatgtgtt ccgactttgc gcagccgctg cgcttggcgc acaccagcga cttcatctac
     1441 atcgatccac cgtatcatcc cgtcagcgcc acctctaatt tcgtgtcgta tacctcgaat
     1501 gggtttggct gggacgagca gaaaagatta gcggactggg tcgcccgcgt gatcgatcgg
     1561 ggctgctacg tgctcatcaa caacgccgac acacccgaag ttcgccagct ctaccgatcg
     1621 gcattcaaat cgcgccggat caaaatccac ctgcaatcgt ttaccactgc ccgcaacatc
     1681 aacagccgcg ccgattatcg cagcggcagc ctcgaactga cgatttggaa ttactgacca
     1741 cgatcagaat tccgaagtct atccaaattg attacggtaa cctgattgaa agaggagaac
     1801 cgtcatgcgc attttgcaat cgccatcaat gttgctgcgt ctcgaaggcg cggccttgct
     1861 aattggaagt gtcatcgcct acatttttct gcacgccagc ggcgggctgt ttgcgctgct
     1921 gatcctcgcg cccgatctat cgatgctcgg ctatgtggcg ggcgtcaaaa ccggatcatc
     1981 gatctacaac ctggtgcaga tcgatctctg gccgatcacg cagatcgttt agggtctagc
     2041 tgtttctggt gcaggggtta tcccgatcgg cttgatctgg ttagcgcaca tcggtggcga
     2101 tcgattatta gggtttggct tgaagtatcc gacggacttt aaagatactc aactgcaaca
     2161 ggtgtaattg acgtgaggga gaatattgtt ataatcagtt catactttgt gtgtcattct
     2221 gagccgcgct ggcggcgaag aatctccgcc aataagcgca gagatttctc gctccgctcg
     2281 aaatgacagc caaggacggg cccgtagctc agtggttaga gctgtcggct cataaccgat
     2341 cggtcgctgg ttcgagccca gccgggccca ctaaacgaaa aaccttgcga aggccatctc
     2401 ctcttcgcaa ggtttttgtt tctaccacac tgataactct tggcccgccg gccgcgagga
     2461 cgcgggcttt gagcaacggt gtctgaaccg tttttctttg cgcacttggc gttctttgcg
     2521 gcgaaattat ttcttacgct gctttgaatt tgatgtccca atgcgtgccg tcacaaaacg
     2581 gtttgttctt cgattgaccg caccggcaca gcagctgtcg attgcgcgct tcgtacgtga
     2641 acccgtccgg cgcttcgatc gggatgccgc ccaacaccca taccggcccg tcgggaatgg
     2701 ccgcaatcat cggctcgtat ttgatctctt gaggtttctc gccgcgcttg agcagatact
     2761 gcaaacgtcc gctggggcat aacggaattt gtttctttaa ctgttcgctg attgccggat
     2821 cggcagtctg attgatttca tcccacacgc cgccgaatcg atcgcaaaat tcaaagcccg
     2881 cgcacaatga gttatcgtcg ctcaagatca tgccggttcc ctgaaaaagt tggcggcgtg
     2941 tttcgcctgg cgcccgatcg gccaccaaag acccatccca ctccacttct tgttcatgat
     3001 gatcatcgca gaacggcata ttgtccgaat gtccgcaccg gcacaatttg taagtcgtgg
     3061 tttcgacggg ataatcctcg ccggattgcc attcgaccgc atcgccctgc gcattccgca
     3121 cgcgaatccg ccgcgtcaac ttgacaccgc ccgtgaccac gtacggcccg tgctcttcaa
     3181 tctggatgtg cggtttagat gactcttctt tttgcggttg cttagcggcg ttgtgcttta
     3241 attggtcagg catggaatag atttcctttc tacttatttt tgaacacctt caaccactca
     3301 gcaacttctt cgctgcttaa cttttcttcc accaccgatc gatcgtcgcc cttcggcggc
     3361 gcggtcatga tcgctacaaa ctcctgcgcc gatttgagca tcgccttgcg cttgaatgca
     3421 acggcccgca cttgattatc ggacgagata acggtccaat cagcgggccg cggaaaatca
     3481 ttgatgcgct cgatcaaaac tcgatcagcg atggtgtgcg tggccgccgc aaagatcgca
     3541 gtgacgccgc cgcccgatag tgcgttcgat cgaccgccgg gaattccggt gtcaaatacg
     3601 acgacgatct tcgcgccacg ccgggctgca tatttgcgca gcatcacgat cagcttttgc
     3661 tcgtcgtcgg gatcgtcgag cgctaggccg ggcacttgtg cgatgaggtt atgaccgtcg
     3721 atgagcagtt gctgcatgat tgattcgttt acgcattcca gtgtacgcac taagcaatgc
     3781 gtagtgcgta ttctgtaaca aacttattaa ccgggcaatt tcgtgagatt cgcaaaactt
     3841 gcgagcaagc gtttgatgcc cgctttctca aacgcgacgg tgacttcttc atcattgcga
     3901 tcaattttgc tttcgatcac gatgccgtca ccgaatttgc catgcttcac ccgttgaccc
     3961 gatcggaata gcatcgctcg ggctggctca gcggatcggg ttttagctga ggccgattcc
     4021 cacgtcgacg cgcgaaccat cgccgcctct ttgggtttcg acccgatgaa ggacatcgat
     4081 ccgctgagca actttttcgg aatgtcgttg aggaaacgcg agggcgtgct gacatcatcg
     4141 ctgccccaca tcgtgcggcg gaatgcgtac gtcagcagca cacgttcttt cgcgcgcgtg
     4201 atgcccacgt agcacaatcg ccgctcttct tccaattgat ccgggtcttc catcgatcgc
     4261 gcatgtggca gcagaccttc ttcaagtccg gtgatgaaca cgatcggaaa ttccaaaccc
     4321 ttcgcggagt gcagcgtcat caacgttggc gcatcagtct gatctttcaa ggcatcgaca
     4381 tcggccacta aggccacatc ctcaagaaat tccgccagcg gatcatcgcc gacggcgcgc
     4441 gtgtaatccg acgcgacgtt gcgcagttcc ttgatgttgt cgtagcgatc tttgccttct
     4501 tccgtgccgt cgtcgatgaa gcgttgatac gcagactctt tcaagatcag atcgatcaac
     4561 tccgcaatat tgctgccgcg cgcgggtgaa tcaatgaacc gatcgatcat cgcggcgaaa
     4621 tcaaccaacg cctgcttggc cttcggcgtc agactggagg cgagcgggct agtcgcgccc
     4681 gccgatttga gttcgtgaat cagttgatag gccgattttt cgccgcgccg agaaacttca
     4741 tccaatacgc tcagcgtctt cgcgccgatg ccgcgcggcg gcacattgat cacgcgcagc
     4801 aaactcacgg cgtcgtgcgg
//
LOCUS       DASRTJ010000713         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_373652,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000713 DASRTJ010000000
VERSION     DASRTJ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373652"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFF70_14450"
     CDS             complement(<1..180)
                     /locus_tag="VFF70_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZY45935.1"
                     /translation="MRYLALGDSYTIGESVAEAERWPVQLTSRLRAEQIDLDEPIIIA
                     KTGWATDELNAAIDQL"
     gene            complement(355..>2807)
                     /locus_tag="VFF70_14455"
     CDS             complement(355..>2807)
                     /locus_tag="VFF70_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45936.1"
                     /translation="GRNQLNGWLALGQKPAGQPFTTEDLTYLSTFADQTALALERAII
                     MNDVQRRVNELNVLSRVSQAVNFTLDPDDILELIYTQTSKVLETQNFYIAMADQKRNV
                     MRFAFYVEGGERLYPDDEWPIGFGLTGDIMRRGQPIVTDDYVAESINRGFTSSGKPGR
                     AWMGVPLNAGDRALGVMSVSDFRPEVSYSSEQMQILAAIADQAASVLDKARLYRETEE
                     RARQLAVLNEVGTNLTSSLDLRTVLSTIVQKAIEILHAEAGSLLLVDEQTNELVFEVT
                     FGPAAPDLRGQRLPFGVGIVGAVAKSRQPQIVNETGSDSRFLPDVDKSTTFNSRAILA
                     VPMIVKDRVTGVIELINKLEGDKFNEEDQNLLTAFATNAAVAVENARLFTITDQALAT
                     RIDELSMLQEIDRQLNQSLDLRTVLDITLEWGLRLADATAGSVGIVDLEQNVVQILTS
                     RNYTYTPAALPIDQGLAGQVINSGQAVLVNDVRQDSHYIAGSPTTQSQLSVPIRRGDR
                     VIGVLNVESSKIDHFGILFLDSVSRLADHAAIAITNANLYQEVRYANEAKSKFVSDVA
                     HELNQPMTSIKGFTDMLIKGMAGSVNETQSDFLARIRFNTERMDRLIRDLLEIGRIET
                     GRLKLDIKSNSIKSVVEDTLRALQSQIDERKMTVELSIPDELPPVMADRARLIQIVTN
                     LVSNAYKYSPNGGKIAIGVSSLKTIQSPDVPRGNWTRSDIKTIKPYAPGYLRCSITDT
                     GVGIAPEDQAKLFTQFFRSGAVRDVPGTGLGLIITKSLVELQHGAIWVETEVGKGSTF
                     TFSLPIAEPEAIPAASAA"
BASE COUNT          609 a          788 c          756 g          654 t
ORIGIN      
        1 gagctgatcg atcgcggcat tcagttcgtc cgtcgcccag cccgtcttcg caatgatgat
       61 cggctcatcc agatcgatct gctccgcgcg cagcctcgac gtcagctgca ccggccagcg
      121 ttctgcttcc gccacgcttt caccaatggt ataagaatca ccgagtgcca agtaacgcat
      181 taacaatcct ttattgatat tctcgaagcg cgcgtcttgc ggacgttgca cagaatccca
      241 ccgcgaggac gcggggcgga gagcaaactg catcatgaga cttaatgaaa tacccgctct
      301 caaatatcaa gagcggatgg tcattcaagc attgtcctca catagtcgcg cggattacgc
      361 ggctgaggct gccggtatcg cttccggttc cgcgatcggc aggctgaagg tgaacgtgct
      421 gcctttgcca acttcggtct cgacccagat cgcgccgtgc tgcaattcga cgaggctctt
      481 agtgattatt aatcctaaac cagtgccggg cacgtcacgc actgcgccgg atcggaagaa
      541 ctgcgtgaac aacttggctt gatcttcggg cgcgatgccc acgccagtat ctgtgatcga
      601 gcacctgaga taacccggcg catacggctt gatcgttttg atatccgatc gggtccagtt
      661 gccacgcggc acatctggcg attggatcgt cttgagtgaa ctgaccccga tcgcaatttt
      721 tccgccgttc ggcgaatatt tgtaggcgtt gctgaccaaa ttggtgacga tttgaatcag
      781 gcgcgcccga tcggccatga cgggtggcag ctcatcgggg attgaaagct caacggtcat
      841 cttccgttca tcgatctgtg attgcagtgc tcgcagcgta tcttccacta cgctcttgat
      901 cgaattggat ttaatatcca gcttcaagcg gccggtttca atgcggccaa tttccagcag
      961 atcgcggatc aagcgatcca tgcgttcggt gttgaaacgg atgcgcgcta ggaagtcaga
     1021 ctgagtttca ttcacgctgc cggccatgcc tttgatcagc atgtcggtaa agcctttgat
     1081 gctggtcatc ggctgattga gctcatgcgc cacatcgctg acgaacttgc tcttggcttc
     1141 attggcgtac cggacttctt gatacaagtt cgcgttggtg atcgcaatgg cggcatgatc
     1201 ggccagacgg gaaacagagt cgagaaacaa gatgccgaaa tgatcgattt tgctgctttc
     1261 aacatttaac acgccgatga cccgatcccc gcgccggatg ggcacgctca actgcgattg
     1321 agtggttggc gagccggcga tgtaatgcga atcctgccgc acgtcgttga ccaacacggc
     1381 ttgtccgctg ttgatgactt gtccggccag gccttgatcg atcggtaaag ctgccggcgt
     1441 ataagtatag ttgcggctgg ttaaaatttg aacaacattt tgttccagat caacgatgcc
     1501 gaccgatccg gcagtcgcgt ccgccagacg caaaccccat tcgagagtga tatcgagcac
     1561 ggttcgcagg tccagcgatt gattgagctg gcgatcgatt tcctgaagca tactgagctc
     1621 atcgatgcgg gtcgccagcg cttgatcggt gatggtgaac aagcgcgcgt tctcaacggc
     1681 gaccgccgca ttcgtcgcga aggccgtgag caaattctga tcctcttcgt taaacttatc
     1741 gccttcgagc ttgttaatca attcgatgac gccggtcact cgatctttga cgatcatcgg
     1801 cacggccaag atcgcgcgtg agttaaacgt ggtggactta tcgacatccg gcaggaaacg
     1861 cgaatccgat ccggtttcgt ttacaatctg cggctggcgc gacttggcga ctgcgccaac
     1921 gatgcccacg ccgaatggca aacgctgccc gcgcagatcg ggcgcggccg gaccaaacgt
     1981 cacttcaaaa accaattcgt tggtttgctc atcaaccaac aacagcgatc ccgcttcggc
     2041 gtgcaaaatc tcaatcgcct tctggacgat ggtactcagc acggttctga gatcgagcga
     2101 ggaggtgaga ttggtgccca cttcattcaa cacagccaat tggcgtgcgc gctcttcggt
     2161 ctcgcgatac agccgcgctt tgtccaaaac actggcggct tgatcggcaa tggcggccag
     2221 aatttgcatt tgctctgaac tgtaactgac ctccggccgg aaatccgaca cgctcatcac
     2281 gcctaaggct cgatcgccgg cgttgagtgg cacacccatc catgcgcggc cgggcttacc
     2341 gctggaagtg aaaccgcggt tgatcgattc agccacataa tcgtccgtca caatcggttg
     2401 accacggcgc ataatatcgc cggttagccc aaacccgatc ggccactcat catccggata
     2461 cagccgttcg ccgccctcga cgtagaaggc aaaccgcatg acgttgcgtt tttgatcggc
     2521 catggcgata tagaagtttt gtgtttccaa tactttgcta gtctgggtat agatcaattc
     2581 aagaatgtca tccggatcga gcgtgaagtt caccgcctga ctgacgcgac tcagcacatt
     2641 caactcattg acgcgccgct gcacgtcatt catgatgatt gctcgctcca atgccagcgc
     2701 agtttgatca gcgaaggtgg atagatacgt gaggtcttcg gtcgtgaaag gttgtccggc
     2761 tggtttctgg ccaagcgcca accagccatt gagctgattt cggccag
//
LOCUS       DASRTJ010000714         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_145933,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000714 DASRTJ010000000
VERSION     DASRTJ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145933"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4165)
                     /locus_tag="VFF70_14460"
     CDS             complement(<1..4165)
                     /locus_tag="VFF70_14460"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZY45937.1"
                     /translation="MAAINPPRPSTNRLPLILIALVAVIVLVGGGLFLLSRRNAAPAA
                     NLAPIVIDRLPDRGEEQPTEAPIVLNFDKPMDRASVESAFDISPKVPGTFKWSTDNKS
                     VQFVPTGSGFERNTTYNVKLLDTAKAANGKTLGQIMAFAFKAVGFLNVTQVIPADGST
                     DIDPQSDITVMFNRPVVPLLAASDQTQLPQPLTFDPPIKGAGEWLNTSIYVFHPSERL
                     QAGVLYTANVAAGLQDPTGAVLQKDFIWKFTTQTPIVLRTSPNDSENSIGLDHAIQVT
                     FNQPMDHASAQAVFALHSGSIDGAKVDGTFTWVTETVFAPQGVGQAGKLAAPALGGAQ
                     PNQTLLGETMIFTPSQLLKRGETYFGVVAAGAKAKGGGAPMQADYTWTFKTVPVFRVI
                     RTDPADGDRNATAYGSIDIYFSGPVKESTLANISVQPAPSITDVYTYYSEYDHHYTFG
                     FAAAPSSDYAVTLGAGVSDPWGQSLGKDQVIHYSTRALDPEQWFDVPGRFGVYSAYTD
                     TVVYVRYRNVTQLDFELYQVSLDQFGVLVGQNSYSLWNSSVPVSGAPIRAWSVPVTTT
                     LNKSGLLRVPVGGEAGGALTTGIYLIRVTSPEQRENDFGGTTRHLLVVANTNVTYKSA
                     EREGLVWATDLQSGDVTPAAPTTIYDGDFKVLAAGQTDASGLFKTDWQSPINTFDTAT
                     AIVGEPGGTFGIAINSWTDGIGPYDFGVNANYFNDPYNVYIYTEKPLYRPGQTVHFRG
                     IARLDDDARYAIDPNLRSIDVVVYDSQGNQVLSSTLPLSDYGSFNADLKLDQEAGLGY
                     YSIQAQIPTGGSNPKGPNVNTYYGQFLVSEYVRPQFQVNVTADKPEVLQGGTANVDVQ
                     ANYFFGGSVNGADVTWTALSNNYYFDRYHGQGYFSWNDTDYFVGPAQARSGPLASGEG
                     QTDASGHFKITLPAKLDEKSGSQTFSIEASITDLNGQQVSGRVSVIVHQGSYYIGIAP
                     QDYVGVAGQPMGFDLRSVDWQGDPSGQRNIDVVFYQREWFNVQEKDDIGNIFWTYSFS
                     DTAVFTTSTTTTEGGEGTVSFTPPVGGEYHVVAIGKDENGNSIRSATGVWVSSRDYIS
                     WRQENNDRIQLVADKKAYNPGDTAKILVPSPYQGKVKALVTVERGRILTSKVINLQTN
                     SDVIEVPITPDMAPNAFVSVVIVKGVDETNLAPSFKMGYASFTVNRVQQVLNITLTPD
                     RDPKAAHYGPREPISYTIKVTDYAGKPVQSEVSLALVDLSVLSLADPLERPIADQYYG
                     ERGLGVQTSTSLVFSVDRINAKLAQEAKGGGGGALAEGGDFFVRGNFPDTAYWNATVT
                     TDANGQATISTVLPDNLTTWRLLAKAISKNTLVGEGQTDVLSTKDLLLEPVTPRFFIV
                     GDKVNLAAVV"
     gene            complement(4209..5117)
                     /locus_tag="VFF70_14465"
     CDS             complement(4209..5117)
                     /locus_tag="VFF70_14465"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HZY45938.1"
                     /translation="MRARVRSWIVLPLLVMLPLSTLACAFVGGAFNSTPIDTTPAATL
                     PPIVSTPLAIGTSIGVQPTAIVLQPTSFPAATQSPSIDSGAKESIAVTLPLPGQGVSG
                     SIHIEGISDPTFEQTLGVLVRDQTGSVIANGSAHINVDVGQRGKFSGDVSIPNGAVQP
                     GRLVVYATSARDGGLTHLASLDVELNSTKPSNITALNPDRGESILITSPAPNASLSKT
                     VTVSGVSDPTFEQSLVVEVRDANNHQLGRATVQIKADAGQRGSFSIDVPFQVSTAQPG
                     RIVVYSLSARDGLTVHLNSVEVHLQP"
     gene            5320..6132
                     /locus_tag="VFF70_14470"
     CDS             5320..6132
                     /locus_tag="VFF70_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZY45939.1"
                     /translation="MNLLVVGGSGYVGSLVLPMLAQHHQLRIFDLRPPVDPAWEYVPG
                     DVRDLDSISHAMTGIDALIYMAMGLKDSPENPREEQIKSGFDVSVKGLYLALLAAQQS
                     QVQAALYLSTISIYGLDTDVERRYFISENLTPEAKSAYGLTKFLGEEVCRFAARNWHM
                     HVNVLRLCWPRSIEDWQAQAYRGTPTPKTSADDLARALLAALQFRQGFQLFNLSGDYD
                     QQIIKMRKAKRLLHWAPLARPIKAQREKISRRVMKRSLRRWLAKFSYAIGRR"
BASE COUNT         1344 a         1774 c         1716 g         1382 t
ORIGIN      
        1 gcacaaccgc ggccagattc actttatcgc cgacgatgaa gaagcgcggc gtcaccggtt
       61 cgagcagcaa atcttttgtg ctcaacacgt cggtctggcc ttcaccgact aacgtgtttt
      121 tgctgatcgc tttcgccaac aaacgccatg tcgtcagatt gtccggcagc accgttgaga
      181 tcgtcgcttg accgttcgca tcggtcgtga cggtcgcgtt ccagtacgca gtgtccggga
      241 agttgccgcg cacgaagaag tcgccgcctt ctgctaacgc gccgccgccg ccgcccttcg
      301 cttcctgcgc aagtttggca ttgatgcgat cgaccgagaa gacgagtgaa gtcgatgtct
      361 gcacgccgag gccgcgctcg ccgtagtatt gatcggcgat cggtcgctcc agtggatcgg
      421 ccagcgatag caccgataga tcgaccagcg ccaaagatac ttctgactga acgggcttgc
      481 ccgcatagtc ggttactttg atcgtgtaac tgatcggttc gcgcgggccg tagtgcgccg
      541 ccttgggatc acgatcgggc gtcagcgtga tgttcaacac ttgctgcacg cgattgaccg
      601 tgaacgacgc gtagcccatc ttgaacgacg gcgcgagatt cgtttcatcc acgcctttca
      661 caatgactac cgacacaaac gcattcggcg ccatatccgg cgtgatcggc acttcgatca
      721 catcgctgtt cgtttgcaag ttaatcactt tcgatgtgag gatgcgcccg cgttcgacgg
      781 tgaccagcgc cttgactttg ccttgatatg gcgatggtac cagaatcttg gccgtgtcgc
      841 cgggattgta agctttctta tcggctacca gctgaatccg atcgttgttc tcctgccgcc
      901 acgaaatgta atcgcggctg gacacccaca ctcccgtcgc gctgcggatc gaattgccgt
      961 tttcatcttt gcctatggcg acgacgtgat attcaccacc gaccggcggc gtgaatgaga
     1021 ccgtgccttc accgccttcg gtggttgtcg tcgatgtggt gaacaccgct gtatcgctga
     1081 acgaatacgt ccagaagata ttgccgatgt cgtctttctc ttgcacgttg aaccattcgc
     1141 gttgatagaa caccacgtcg atgttgcgct ggccggacgg atcgccctgc cagtccactg
     1201 atcgcagatc gaagcccatc ggttgtccgg cgacgcccac gtaatcttgc ggcgcaatgc
     1261 cgatgtagta actgccttga tgcacgatca cgctgactcg accggagact tgctgcccgt
     1321 tgagatcggt gatcgaggct tcgatgctga atgtttggct gcccgatttc tcatccaact
     1381 tggcaggcaa cgtgatcttg aaatgtccgc tcgcatctgt ctggccttcg cccgaagcca
     1441 gcggccccga tcgagcttga gctggcccga cgaaataatc ggtgtcattc caactgaagt
     1501 aaccttgacc gtgatagcga tcgaagtagt agttattgct gagtgccgtc catgtcacat
     1561 ccgccccatt gaccgatcca ccgaagaagt agttggcctg cacatcgacg ttggctgtgc
     1621 cgccttgcag cacttccggt ttatccgcag tgacattcac ttggaattgc ggccgcacat
     1681 attcagatac caagaactga ccgtagtagg tgttgacgtt aggacctttt ggatttgatc
     1741 cgcctgtcgg gatttgagct tgaatcgagt aatagcccag acccgcttct tgatcgagtt
     1801 tcagatcggc attgaatgat ccgtaatcgc tcaagggcaa agttgaactc agcacttgat
     1861 tgccctgcga atcgtacacg actacatcaa tcgatcgcag attcggatca atcgcgtagc
     1921 gcgcatcatc atccaagcga gcgatgccgc ggaagtgcac ggtctgcccc ggtcgataca
     1981 acggcttctc ggtatagatg taaacgttgt atggatcgtt gaagtagttg gcattcacgc
     2041 caaagtcata cgggccgatg ccatccgtcc aactattgat cgcgatgccg aatgtgccac
     2101 ccggttcgcc gacgatggcc gtggccgtat cgaacgtatt gatgggggac tgccaatcag
     2161 tcttgaataa accggaagca tcggtttggc cggccgccag aactttgaag tcgccgtcgt
     2221 aaatagtggt cggcgcggcg ggtgttacat cgcccgattg caaatcggtc gcccacacca
     2281 agccttcacg ctcagcggat ttgtaagtca cgtttgtatt cgcgacaacc aacagatgcc
     2341 gcgtcgtgcc gccgaaatcg ttctcgcgtt gttccggcga agtgacgcgg atcaaataaa
     2401 tgccagtcgt caacgcgccg cctgcttcac cgccgaccgg cacacgcagc agaccggatt
     2461 tgttaagcgt cgttgtcaca ggcacagacc acgcgcggat cggtgcgccg gacaccggca
     2521 ctgaactgtt ccataaactg tacgaatttt gtccgacgag cacaccaaac tgatcgaggc
     2581 tgacttgata caactcgaag tccagctgcg tgacattgcg atagcgcaca tacacgacgg
     2641 tatccgtata cgcgctgtac acgccgaagc ggcccggcac atcgaaccac tgctccggat
     2701 cgagcgcgcg tgtgctgtag tgaatcactt gatccttgcc cagactctgg ccccacggat
     2761 cgctgacgcc ggccccgagc gtgactgcat aatccgacga gggtgccgcc gcgaaaccga
     2821 aggtgtaatg gtgatcgtat tcgctgtagt aggtgtacac atcggtgatc gatggcgcgg
     2881 gctggacgct gatattggcg agcgtgctct ctttcaccgg cccggagaag taaatatcga
     2941 tcgagccata agccgttgca ttgcgatcgc cgtcagccgg atcggtgcga atgacacgga
     3001 agacgggcac cgtcttaaac gtccaggtgt aatcggcctg catcggcgcg ccgccgcctt
     3061 tcgctttcgc gcccgcagcg accacaccaa aataagtctc gccgcgcttg agcagctgcg
     3121 atggcgtgaa gatcattgtt tcgcctaaca gcgtttgatt tggttgagcg ccgcctaatg
     3181 cgggcgcggc cagtttgccg gcttgaccca cgccttgcgg cgcgaagacc gtttcagtca
     3241 cccacgtgaa agtgccgtcc actttcgcgc cgtcgatcga tccgctgtgc agcgcgaaca
     3301 ccgcttgcgc gctggcatga tccatcggtt gattgaacgt cacctggatc gcatgatcga
     3361 ggccgatgct attttcagaa tcattcggcg aggtccgcaa gacgatcggt gtttgggtag
     3421 tgaacttcca aatgaaatcc ttttgcaaca ccgcgccggt tggatcttgc aaacccgccg
     3481 caacattcgc ggtatacaac acaccggcct gcaaacgctc gctcgggtgg aacacataga
     3541 tcgaagtgtt caaccattcg cccgcgcctt tgatcggcgg atcgaacgtc aacggttgag
     3601 gcagctgcgt ctgatcggat gccgccagca gcggcactac gggccgattg aacatcacgg
     3661 tgatatccga ctgcggatcg atgtcggtcg atccatcggc gggaatcacc tgcgtgacat
     3721 tcaagaagcc gacggctttg aaggcgaagg ccatgatttg tcccaacgtt ttgccgttcg
     3781 cagctttggc cgtgtcgagc agcttcacat tgtaagtggt gttgcgctca aagccgctgc
     3841 cggttggcac aaactgcaca ctcttgttgt cagtgctcca cttaaacgtg ccgggcactt
     3901 tgggcgaaat atcgaacgcc gattcgaccg aggcgcgatc catcggcttg tcaaagttca
     3961 acacgatcgg cgcttcggtg ggttgctctt cgccccgatc gggtaaacga tcgatcacga
     4021 tcggcgccag gttcgccgcc ggcgcggcat ttctgcgcga caacaaaaag agtccgccgc
     4081 cgaccaatac gatcacggcg accaatgcga tcaagataag cggtaatcga ttggtagaag
     4141 gacgaggggg attgatagct gccatgagac tccttaaaat gatgagtgaa aagtgaattg
     4201 atcactcttc agggttgtag atgaacttcc acagaattca aatgcaccgt caagccatcg
     4261 cgtgcgctga gcgaatacac tacaattctt cccggctgag cggtcgaaac ttgaaacggc
     4321 acgtcgatcg aaaatgatcc gcgctgaccg gcgtccgctt taatctgcac cgtcgctcga
     4381 ccgagttgat gattgttcgc gtcgcgcact tcgacgacca gactttgctc gaacgtcgga
     4441 tcgctcacac cgctgactgt caccgtcttg ctgagtgatg cgttcggtgc gggcgatgtg
     4501 atgagaatgg actcacctcg atcgggattg agcgccgtga tattagacgg cttagtgctg
     4561 ttcaactcaa catctaatga agcgagatga gttaaaccgc cgtcgcgcgc tgaagtcgcg
     4621 tagaccacaa gacgaccggg ctgaactgcg ccatttggaa tagacacatc gcctgaaaat
     4681 tttccgcgct gccccacatc tacattgatg tgcgcgctgc cgttcgcaat gacgctgccc
     4741 gtttgatcgc gcaccaacac accgagcgtt tgctcaaacg tgggatcgct gatcccttca
     4801 atgtggatcg acccgctcac gccctgaccg ggcagcggca gtgtgacggc gatgctttct
     4861 ttcgcgccgc tgtcaatcga tggcgattga gttgccgccg gaaacgaggt cggctgcaaa
     4921 acgatcgcag ttggctgcac tccgatcgag gtgccgatcg ccaacggtgt tgaaacgatc
     4981 ggcggcaggg tcgcggccgg ggtagtatcg atcggggttg agttaaacgc gccgccgaca
     5041 aatgcgcaag ccaatgtact cagcggcagc atcactaata atggcaggac tatccaagat
     5101 cgaacacggg cacgcatgcc agctccttat cagtatcata acgccgaatg cgctcggcaa
     5161 gttcccgcga agcctacgat cgatcggcgc gcagcccacg attgatcgac gctggaaact
     5221 ttgatctgat catagcctac tctaaatcaa tctgcaagat gctgagggcg attacacgct
     5281 atcatcggca cacagattga tcgatgtata atgtgacgca tgaatttatt ggtggttggc
     5341 ggatcaggct acgtgggcag tctcgtgctg ccgatgctcg cgcagcatca tcaactgcgc
     5401 attttcgatc tgcgtccgcc ggtcgatccg gcctgggagt atgtaccggg tgatgtgcgc
     5461 gatctcgatt cgatctcaca cgcgatgacg ggcatcgatg cgctaatcta catggcgatg
     5521 ggcttgaaag actcaccgga aaatccacga gaggagcaga tcaaatctgg cttcgatgtg
     5581 agcgtgaaag gtctgtatct ggcgctactg gccgcccaac aatcacaggt tcaagcggca
     5641 ctttatctca gcacgatatc catttatggt ctcgatactg acgtcgagcg ccggtacttc
     5701 atcagcgaaa atttaacacc cgaggctaaa agtgcctacg gtcttaccaa atttctaggc
     5761 gaggaagtct gccgctttgc ggcacggaac tggcacatgc acgtcaacgt gctgcgcttg
     5821 tgctggccgc gatcgatcga ggattggcag gctcaagcct atcgcggcac gcccacgccc
     5881 aagacttcag ccgacgatct cgctcgcgct ttactcgccg cgctgcaatt ccgtcagggt
     5941 tttcaactct tcaacctcag cggcgattac gatcaacaga tcatcaaaat gcgcaaagcc
     6001 aaacgcctgt tacattgggc gccgttggcc cgtccgatca aggctcagcg tgaaaagatc
     6061 agccggcggg tgatgaagcg atcgctgcga cgctggctgg ctaaatttag ttatgcgatc
     6121 gggcggcgat gatcttcgag tttaggcgac acacgcaatt taggctataa tccccttacg
     6181 aacgaatcga cgtccacatt tgccctcaag gactgg
//
LOCUS       DASRTJ010000715         4755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_146250,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000715 DASRTJ010000000
VERSION     DASRTJ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146250"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFF70_14475"
     CDS             complement(<1..260)
                     /locus_tag="VFF70_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZY45940.1"
                     /translation="MATGFADINSGKLFFELEGQGTPLVMIHGGLVNHHLWDEQFEYF
                     VHHGHQVLRYDVRGYGQSSMPDRSFSHQGDLKLLMDSLGIERA"
     gene            complement(322..2886)
                     /locus_tag="VFF70_14480"
     CDS             complement(322..2886)
                     /locus_tag="VFF70_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZY45941.1"
                     /translation="MPSSSDSSSQSNFISSQESLSTQVIVSARANLVVRQADGSPRMI
                     PLKREPLTIGRHPTNTVVIDIATVSAEHAIIENIDGAYRLTDRNSKNGTFVNGQRIGV
                     TMLHDGDIIRIGDARGNSVSLTFHAGHAPQATSLETFDLATFDRLMIGRDADCDLTLD
                     SPLVSRQHARLERSGSTHTLIDLNSTNGTFVNSQRIDRVQLQTGDIVQIGPYRIIYHP
                     NEMSQVLNTQRGRLDALHLTRRVGADRIILNDVSLSIAPREFVAIVGASGSGKSTLLN
                     ALSGFERAEGRVLLNGDDFYGHFDLYRNLIGYVPQDDIIHRELPVGQALRYTAQLRLP
                     GDTSSHEIDTRIDRVLAEVELEAQHDQIVGQLSGGQRKRVSIGAELLAEPGVFFLDEA
                     TSGLDPGLEKKLMYTLRRLADGGRTIVLVTHATANITQCDHVAFLSEGQLVFFGPPSE
                     ALEFFGVKEFADIYGEIERDPQWWEKRFRESSVYERYVLSRLQRIDQPRSAHATGATR
                     RKTNDKPLAEVASRASPIAPRANALRQFSILIQRYFELVVRDKVLLTVLLAVMPLVGI
                     LLATIAQPTALVGDSVDRIAQIVSEKGLYTIAGQTQTVLMMLALAAVLVGLFAASFEL
                     VRERAIYRRERMINLRLIAYIASKLTVLVGFATLQCMALIVVLAFRIQFPSAGILLPG
                     PLEIYTTLLLASIAGILLGLFISAIVSSVNTVIYVVLLAVFVQIIFGGVIFDLPGAAK
                     PLSYFTITRWTVEALGSTVDLPRLNNLGQIEVHRTIDTIDPLTGAKIQREVVYQDRLP
                     ATFTVDYAHTPDWLISRWAILLAFSIVLGALTALAQSRWSPTGRKGIERRRRRFRR"
     gene            complement(3095..4597)
                     /locus_tag="VFF70_14485"
     CDS             complement(3095..4597)
                     /locus_tag="VFF70_14485"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZY45942.1"
                     /translation="MNTRIVRFLFGATIALAAALSLRILPMPALTRAAPSTTFIVNSN
                     ADAPDATLNGVCETGNGNGICTLRAAITEANAASGSTIVFGQQGSLTYLLTLGELNIT
                     ANVKIVGNGSANTIIDGNGSVTNSRVFDISSSSLVVNMTGVTIRNGKANQGGGINNLG
                     KLSLSHSTVRDSVAAAIDPRSGGGIYNTGILTVTNSFINDNSAPNVSFTGGGGGIYNS
                     GGTVVLINSTLNSNSTNSTGGAIQVDGGTVSLVNSMVNSNIANYGGGIFNGGTLNITQ
                     SGIYSNTASNSSFSGGGGGIDNFNGVATIYNSTIYLNVANRDGGGIGNESSGTLYLYN
                     VTVAGNYSDWDGNNTGVGGGIYQGGGSVNFRNTILAGNYNWSNSCITCSTPDDCAGTL
                     NSEDYNLIQTTSGCTLNAPGHDITGVDPQLSYPPHFNGGPTLNLALLPGSPAIDAGNT
                     GGCRDGLGALIPTDQRGFPRPANGGVNGLRCDIGAYEWYRFGVFLPLIRK"
BASE COUNT         1089 a         1304 c         1306 g         1056 t
ORIGIN      
        1 gcgcgttcaa tgccgagtga atccatcagc aatttcagat cgccctgatg cgagaaagat
       61 cgatcgggca tggaggattg gccgtagccg cgcacatcgt aacgcagcac ttgatggccg
      121 tgatgcacga agtattcgaa ctgctcgtcc cataggtgat gattgaccaa gccgccgtga
      181 atcatgacca gcggcgtgcc ttgtccttcc aattcgaaga acagtttgcc gctgttaata
      241 tctgcgaaac cagttgccat gcgtgcctct caaaaagaat gcggttgatg aaaagtgatt
      301 cgtgatatgt aatccgtaaa actatcggcg aaatctgcgc cttctgcgtt cgattccttt
      361 tctgcctgtg ggcgaccatc ttgattgcgc gagcgctgtt aacgcgccga ggacaatcga
      421 gaatgcaagc aagatcgccc agcggctgat caaccaatcg ggcgtatgcg cataatcgac
      481 ggtgaaagta gccggcaatc gatcttgata gaccacctcg cgctgaatct tcgcgccggt
      541 caacggatcg atcgtatcga tcgtgcgatg cacttcgatc tggcccagat tattcaagcg
      601 gggcagatcg actgtgctgc ccagcgcttc gaccgtccag cgcgtgatgg tgaagtacga
      661 gagcggtttg gcggcgcccg gcagatcgaa gatcacgccg ccgaaaataa tctgcacaaa
      721 aacagccaat aataccacgt agatgactgt attgacactg ctgacgatcg cggagatgaa
      781 taagccgagt aagatgccgg cgattgaagc caggaggagc gtggtataaa tttccagcgg
      841 ccccggcaat aatattccgg cggagggaaa ttggatgcgg aaggctagca cgacgatcaa
      901 tgccatgcac tgcagcgtgg cgaaaccgac gagcacagta agcttcgatg cgatgtacgc
      961 gatcagccgc aaattgatca tgcgctcgcg gcggtagatc gcgcgctccc gcaccaattc
     1021 gaacgaagcg gcaaacaagc ccaccaacac ggcggccaac gcgagcatca tcaacaccgt
     1081 ctgcgtttgt ccggcgatag tgtacagtcc cttctctgaa acgatctgcg cgatgcgatc
     1141 tacggaatcg ccgacgagcg cggtgggttg ggcgatcgtc gctaacaata tgccaaccag
     1201 cggcatgacg gcgagcagca ccgtcaacaa tactttgtcg cggacgacca attcaaagta
     1261 acgttggatc agaatagaga attgccgcag cgcgttggcg cgtggggcga tgggcgaagc
     1321 gcgtgaggcg acttccgcta agggtttgtc attcgtcttt cgtcgcgttg cccccgtagc
     1381 gtgagcggag cggggttggt cgatcctttg caaacgtgac aacacatacc gttcgtaaac
     1441 gctcgactcg cgaaagcgtt tttcccacca ctgcggatcg cgttcgatct cgccgtagat
     1501 atcggcaaat tctttgacgc cgaaaaattc cagcgcttca ctcggaggac cgaagaacac
     1561 caactggcct tcactcaaga acgccacatg atcgcattgt gtgatgttcg ccgttgcatg
     1621 cgtaaccaac acaatcgtcc gacccccatc ggccaggcgg cgcagcgtgt acatcaattt
     1681 cttttcaaga cccggatcga gtcctgaagt ggcctcgtcg agaaagaaca caccgggttc
     1741 agccaacagc tccgcgccga tcgaaacacg cttgcgctga ccgccgctga gttgtccgac
     1801 gatctgatcg tgctgcgctt ctagctcgac ttcggctaac acccgatcga tccgtgtgtc
     1861 gatctcgtgc gacgatgtat cacccggcag ccgcagctgc gcggtatagc gcagcgcttg
     1921 cccgaccggc agttcgcggt gaatgatgtc atcctgcggc acgtaaccga tcaagttgcg
     1981 atacagatcg aagtggccgt agaagtcatc gccgttcaac agcacgcggc cttccgcgcg
     2041 ttcgaagccg ctcaacgcat ttaataaagt tgatttgccc gatcctgatg caccgacgat
     2101 tgccacaaat tcgcgcggcg cgatcgacag cgacacgtca ttcaagatga tgcgatccgc
     2161 acccacacgg cgcgtcaggt gcagcgcatc caggcggccg cgctgagtgt tgaggacctg
     2221 actcatctca ttcggatgat agatgatgcg gtatgggccg atctggacaa tatcgccggt
     2281 ttgcagctgc acccgatcga tccgctggct gttcacgaac gtgccattcg tgctgttgag
     2341 atcgatcaaa gtgtgtgtgg aacctgatcg ttccaagcgc gcatgctggc gcgagaccag
     2401 cggcgaatcg agcgtgaggt cacaatcggc atcgcggcca atcatcaatc gatcgaacgt
     2461 ggcgagatcg aacgtctcta acgaagtcgc ttgcggcgcg tgacccgcat ggaaagtcag
     2521 gctgacggag ttgccgcgcg cgtcgccgat gcggatgata tcgccgtcgt gcagcatcgt
     2581 cacgccaatg cgctggccgt tgacgaacgt gccgttcttg ctgttgcgat cggtcaggcg
     2641 atacgcgcca tcgatattct cgatgatcgc gtgttcggcg gacacggtcg caatatcgat
     2701 cacaaccgtg ttagtcggat gccgtccaat ggtcaaaggt tcgcgcttca gcggaatcat
     2761 gcgcggcgat ccatcggctt ggcgcacgac gaggttcgcg cgcgcggaga cgatcacttg
     2821 tgtgctgaga gattcttgag atgaaatgaa gttggattga gacgacgaat ctgaagatga
     2881 aggcattcca caccgaagta aatgggtctg atgagatgcg aatgcctata ctataccatt
     2941 actttatcct gtttatttca taactgatgg caggaacaac gcggcgcgtt catatttgaa
     3001 cgtgccactg ttcgggataa acgacacgtg caagcaccct gagaacctga gtcgcgtcaa
     3061 cggctggaga ttttcttgaa aaattcagcg catgtcactt gcgaatcagc ggcaagaaca
     3121 cgccaaagcg ataccactca tacgcgccga tatcgcagcg caaaccgttc acgccgccgt
     3181 tcgccggtcg agggaaaccg cgttgatcgg ttgggatcag tgcgccaaga ccatcgcgac
     3241 aaccgcccgt gttgccggca tcgatcgctg gactgcctgg aagcagcgca agattcaacg
     3301 tagggccgcc gttgaaatgc ggaggataac tgagttgtgg gtcaactccg gtgatgtcat
     3361 gccccggtgc attgagcgtg cagccactgg tggtttggat caggttataa tcctccgaat
     3421 tgagcgtgcc cgcgcagtca tcgggcgtac tgcaagtaat gcacgagttg ctccagttgt
     3481 agttgcccgc gagaatcgtg ttccgaaaat tgacactacc gcctccttgg taaatgcctc
     3541 caccgacacc cgtattgttc ccatcccaat ccgaatagtt tcccgcaacc gtcacattgt
     3601 agaggtacag agttccactg ctttcattgc ctattcctcc gccatcgcga tttgccacat
     3661 tgagatatat tgtgctgttg taaatagttg ccacgccatt gaagttgtca atgccgccgc
     3721 caccaccaga gaagctgcta ttcgaggcgg tgttactata aatcccactc tgtgtgatgt
     3781 tcaacgttcc gccgttaaag attccaccac cgtaattggc gatgttgctg ttcaccatgc
     3841 tgttgacaag cgacactgtg ccaccatcaa cttgaatcgc gccgcctgtg ctattggtgc
     3901 tgttgctgtt cagcgtacta ttgatcaaca ccaccgtgcc cccgctattg tagatgccgc
     3961 ctccgcctcc cgtaaaggaa acatttggag cagagttgtc gttgatgaaa ctatttgtaa
     4021 ctgtgagtat gccggtgttg tagattccac caccgcttct gggatcaata gcagccgcaa
     4081 cactgtcgcg cacggtggaa tgtgaaagcg aaagtttgcc caggttgttg atcccgccgc
     4141 cttgattggc tttgccattt cggatcgtca cccccgtcat gttcacgaca aggcttgacg
     4201 aagaaatatc aaacacgcgg ctattcgtca cactgccatt gccgtcaatg atcgtgttgg
     4261 cagaaccatt accgacgatt ttcacattgg cagtgatatt tagttcgccg agtgtcaaca
     4321 gatacgtgag actgccctgc tggccaaaga cgatggttga gccgctggcc gcgttcgctt
     4381 ctgtgatcgc cgcacgcaaa gtgcagatgc cgttgccgtt tcccgtttca cagaccccgt
     4441 taagagtggc gtctggtgca tcggcattgc tattgacgat gaaagtggtg gatggcgcgg
     4501 cgcgcgtcag cgcgggcatt ggcaaaatgc gtagcgacaa tgcagctgcc aaggcaatgg
     4561 ttgcgccgaa gagaaagcgg acgatgcgtg tgttcatgtc attacctccc agtcgactta
     4621 catctatata tacttgtact tgcgtgttac atcgttcatc tattttttga ccagcggcaa
     4681 aaatgatttg acctgatact caaacgcgcc gatgtcgcag acgccgatgc gggagacacc
     4741 gcgctggtct ttcgc
//
LOCUS       DASRTJ010000716         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_299234,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000716 DASRTJ010000000
VERSION     DASRTJ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299234"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFF70_14490"
     CDS             complement(<1..184)
                     /locus_tag="VFF70_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45943.1"
                     /translation="MISRRVWLFIGVAVLVYSVGLAFNLSPYIRGPEEWRWPLAAIPH
                     WDQLWPIGLALLFIAVF"
     gene            complement(181..1386)
                     /locus_tag="VFF70_14495"
     CDS             complement(181..1386)
                     /locus_tag="VFF70_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY45944.1"
                     /translation="MSTLSVIIPAYNEEDGIASVIDRVWSIESTLRESAVDLELIIVD
                     DGSKDRTAEVVKQYPRVRLIQHAVNDGYGAAIKTGFNAAAGEYLAFLDADGTYPPEHL
                     PNLYRALIDQQADLVIGSRMAGASTEMPFVRRVGNMIFAHLVSLLGRQYVSDSASGLR
                     LFKRNILSQLYPLPDGLNFTPVMSTRAVHEHLKIIEVAVPYKERIGRSKLNVVRDGVR
                     FTNSIVWTVLSYNPVRVFGLLGMGLIGLAFLFGVRLLIERVQGITQLDAFGVFSVYAM
                     LVLSVSGVSLFVLGVTFNYLISLLQNRPVRQGLFGKPMFKVPLEQRFGWLGIVAVIAG
                     IAIGGAVWLTGYPIERLWLYLLISALLILSGLQLIIAWLLVKVLSEVKQREVKVKEDM
                     AGSEVKREQ"
     gene            complement(1479..2204)
                     /locus_tag="VFF70_14500"
     CDS             complement(1479..2204)
                     /locus_tag="VFF70_14500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZY45945.1"
                     /translation="MKTIWLYVMRQAATPLHYFWEQLWQVLIGWVPTLIGIGLRGLFY
                     RLILRMDGLAAIENGVRLRFASYIRLGPRSYLDQSVYIHACPAGVRIGHDTLIMHGSI
                     LHVYNFRDLPHAGITIGAQCLIGEYNVIRGQGGVTIGDRVYTSPLVQIVAVNHVFDDP
                     HKPFIDQGITALGIVIEDDVWIGSGAVITDGVHIGRGAVVAAGAVVTSDVAPHTIVGG
                     VPAHLIRTIDGSQTIGDKDVYFL"
BASE COUNT          586 a          690 c          566 g          451 t
ORIGIN      
        1 cgaatacggc gataaagagc aatgccagcc caatcggcca taattgatcc cagtgaggaa
       61 tagccgctag tggccaacgc cactcttctg ggccgcgaat gtacggcgaa agattaaacg
      121 ccaacccgac tgaatagact aaaacggcaa caccgataaa cagccacacc cgccgtgaga
      181 tcattgctcg cgcttcactt cactccccgc catatcttct ttcactttca cttcacgctg
      241 tttcacttca ctcaagacct tgaccaacag ccacgcgatg atcaactgca aacccgatag
      301 aatcagcagc gcgctgatta gcagatacag ccacaaccgt tcgatcgggt atccggtcag
      361 ccaaaccgct ccaccgatcg cgattccggc gatcacggcc acaatcccca gccagccgaa
      421 gcgctgctcc aatggcactt tgaacattgg cttaccgaac aagccttgtc gaaccggccg
      481 attctgcaag agtgaaatca aataattgaa agtcacgcct aaaacaaaca ggctcacacc
      541 cgaaacactt aacacgagca tggcatacac gctgaacacg ccaaacgcat ccaactgcgt
      601 gataccttga acgcgttcga tcagcaatcg cacgccgaac aagaatgcca ggccgattaa
      661 gcccatcccc agcaagccga acacacgcac gggattgtag ctcagcaccg tccagacgat
      721 cgagttggtg aagcgcacgc cgtcgcgcac gacattcaat ttcgatcgac cgatgcgctc
      781 tttatacggg acagccactt cgataatttt gagatgctca tgcaccgcgc gcgtgctcat
      841 caccggcgtg aaattcaaac catccggcag gggatacaat tgcgagagga tgttacgctt
      901 aaatagtcgc aagccgctgg cgctatcgct cacgtactgc cggcccaata agctgaccaa
      961 gtgcgcaaaa atcatgttgc ccactcgtcg aacgaacggc atttctgttg acgcgcccgc
     1021 catacgcgaa ccgattacca ggtcggcctg ctgatcgatc agcgcgcggt acaaattcgg
     1081 cagatgctcc ggcggataag tgccatccgc atcgaggaac gcgaggtatt cgcccgccgc
     1141 cgcgttgaag ccagtcttga tcgccgcacc ataaccatcg ttgacagcat gctgaatcaa
     1201 gcgcacacgt ggatactgct tgaccacttc cgccgtgcga tctttcgagc cgtcgtcgac
     1261 gatgatcaat tccagatcga cggcgctttc acgcagcgtg gattcgatcg accacacccg
     1321 atcgatcacg gaggcaatac cgtcttcttc gttataggcg gggatgatga cagagagtgt
     1381 ggacacggtt gggttctcga caagatgaag ggatgatacg ctcatatgat gccattgctt
     1441 acgagatcat catgtcagca tatgatcaag tcatcttgtc acagaaaata cacatcttta
     1501 tcgccgattg tctggctgcc atcgatcgtt cggatcagat gcgcgggcac accgccgacg
     1561 atcgtgtgtg gcgcgacatc ggacgtgacg acggcgcccg ccgcgacgac ggcgccccgg
     1621 ccgatgtgga caccatccgt gatgactgcg cctgagccga tccatacatc gtcttcgatc
     1681 acgatgccca gcgcggtgat accttgatcg atgaagggct tatgcggatc gtcgaagacg
     1741 tgattgaccg ccacaatctg caccagtggc gaggtataca ctcgatcgcc gatggttaca
     1801 ccgccctgcc cgcgaatcac attgtattcg ccgatcaaac actgcgcgcc gatcgtgatg
     1861 ccggcgtgcg gcaaatctcg aaaattatac acatgcaaga tcgagccgtg catgatcaaa
     1921 gtatcgtggc ctatgcgcac gcccgccggg cacgcatgga tgtagacgga ttgatcgaga
     1981 tagctgcgcg gacccaatcg gatataactg gcaaaccgta gtcgcacgcc attctcgatc
     2041 gccgccagac catccatccg caagatcaat cgatagaata agccgcgcag gccaataccg
     2101 atcagcgtgg gcacccagcc gatcagcact tgccacagct gctcccaaaa ataatggagc
     2161 ggtgtggccg cttgacgcat gacatacaac caaatcgttt tcatatcgct cagctaagta
     2221 cgcacagggc aaccgtgatg ggtgagattc gttcgattcg tatcccaagc gttttcgatt
     2281 ataccagagt gat
//
LOCUS       DASRTJ010000717         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_374355,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000717 DASRTJ010000000
VERSION     DASRTJ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374355"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..526
                     /gene="arsD"
                     /locus_tag="VFF70_14505"
     CDS             167..526
                     /gene="arsD"
                     /locus_tag="VFF70_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HZY45946.1"
                     /translation="MTDTITMFDAAPIHTQATVVEFFDPPMCCPTGLCGPTLDQTLLD
                     VSEMIVALQTEGLKVERYQMTSHPQAFLSNPDVMRLVREKQMAALPITVVRGQVVKVG
                     AYPTLSEIQTYLNGASV"
     gene            523..2415
                     /locus_tag="VFF70_14510"
     CDS             523..2415
                     /locus_tag="VFF70_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HZY45947.1"
                     /translation="MSTQFIFFSGKGGVGKTSLACTQAVRYADEGQSTLIVTTDPASN
                     LADVFEQSIGHQITPIEHVPNLWAMEIDPDRATEEYIDRAMAPIRAAFPLPMVQVMEE
                     QMSGPCTAEVAAFDRFTDFLDVPTDHGQAYDIVIFDTAPTGHTLRLLELPAEWSQSID
                     AASEGSGQTCIGPAAAIQDAKHKYERAMAIMRDPAQTTFVFVLHAEAIAIKETQRATT
                     ELGKLDIHTHELIVNGLIPPEATSNPLFAARAAMQARYLQQVETELSLPTRRMALLSG
                     EIKGVDRLREVGRMFFDGVKEMANQPVNASAKIHIQQPALEAEILSRILPTGQRRAIF
                     FAGKGGVGKTVASCITAVWLAQQGYKTLLLTTDPAAHIGDVLGKPVGDDAAPVEGVKN
                     LWAAKIDPKAAADVYKQRILDDARQRGRPEAAIKVMIEELDSPCTEEMAAFDKFIDFA
                     SQADWQAIVFDTAPTGHTMRLIELPMDWSQQIDVKVFASVDTTAADDVAKQRFGDVIN
                     MMRDSARSTFAFVMYPESTPILEAYRAAEELRTVGIKPGMVVANFVIPAERATTPFTQ
                     SRRVMQEKYLGEIAQRFAVPVVQVPLLPYEVKGIPMLIELGQQVYGLGVDVEAAPLVH
                     SLITAE"
     gene            2431..>2783
                     /locus_tag="VFF70_14515"
     CDS             2431..>2783
                     /locus_tag="VFF70_14515"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HZY45948.1"
                     /translation="MTNQSPAYSINNSALNELELASPEVVRQSARGFAVALGETPQYQ
                     TFDAAVQRLYADEVAMVALEAYQTLQRSLQTMFILNAVSDHDRAELERLRRAYAARPA
                     VKAFAQAQAELISLC"
BASE COUNT          640 a          739 c          783 g          621 t
ORIGIN      
        1 cagctggtgt gttcgggccg cattccgaat cggaccgaag tgacgacgtg gattacgact
       61 gcgctggaac atcaatctca agccgtgtga cacactggtc aagctgagcg agatcgcagt
      121 gaattgattt tttcatccaa ttatttcaaa ggaagagaga ataattatga ctgacacgat
      181 taccatgttt gatgctgcac ccatccatac tcaagccact gttgtggaat ttttcgatcc
      241 gccgatgtgc tgtccgaccg gcctgtgcgg cccgacgctt gatcaaactt tgctggatgt
      301 cagcgagatg atcgttgcac tgcaaacgga gggtttgaaa gtcgagcgct atcaaatgac
      361 cagccatccg caggctttcc taagcaaccc cgacgtgatg cggctggtgc gcgaaaagca
      421 aatggccgca ctgccgatca cggttgtgcg cgggcaagtc gtcaaagttg gcgcgtatcc
      481 aaccctgtcc gaaatccaaa cctatttgaa tggagcaagt gtatgagtac ccagtttata
      541 tttttctcag gtaaaggcgg tgtaggcaaa accagcctcg cttgtacgca agccgtgcgc
      601 tatgccgatg aagggcaaag cacattaatt gttacgaccg atcccgcgtc gaacctagcc
      661 gatgtgtttg aacagtcgat cggtcatcag ataactccca ttgagcatgt gccgaatttg
      721 tgggcgatgg agatcgatcc tgatcgtgcg acggaagaat atatcgatcg ggcgatggct
      781 ccgattcgcg cagccttccc actgccgatg gtgcaagtga tggaagagca gatgagcggg
      841 ccgtgcaccg ctgaagtcgc cgcgttcgat cgcttcactg attttttgga tgtgcccacc
      901 gaccacggcc aagcttatga catcgtcatc ttcgatactg cgccgaccgg tcataccttg
      961 cgcttactcg aactgcctgc cgaatggagt caatcgatcg atgcggccag cgaaggtagc
     1021 gggcaaacct gcatcgggcc agctgccgcg attcaagatg ccaaacacaa gtacgagcgc
     1081 gcgatggcga tcatgcgcga tccggcgcag accacttttg ttttcgtctt gcacgcggaa
     1141 gcgatcgcga tcaaagagac gcagcgtgcc acgactgaac tcggcaagtt ggacattcac
     1201 acgcatgaat tgatcgtgaa cggtctcatt ccgcctgaag ccacgagtaa tccgttgttt
     1261 gctgcgcgcg ccgcgatgca agcccgttat cttcaacagg tcgaaaccga actgtcgctg
     1321 ccgacgcgcc gcatggcgct gttgtccggc gaaatcaaag gtgtcgatcg cctgcgcgaa
     1381 gtgggccgga tgtttttcga tggtgtcaaa gagatggcga atcagccagt caacgcatca
     1441 gcgaagattc atattcaaca gccagcgctc gaagcagaaa ttttgtcgcg catattgccg
     1501 actggtcaac gccgcgcgat ctttttcgcg ggcaaaggcg gcgtaggcaa gaccgtcgcg
     1561 tcgtgcatta ctgcggtgtg gctggcacag cagggttata aaaccttgtt attgacaacc
     1621 gatcccgccg cgcacattgg cgacgtactg ggtaaaccag ttggtgatga tgccgcgcca
     1681 gttgaaggtg ttaagaattt atgggcggcc aagatcgatc cgaaagctgc cgccgatgtt
     1741 tacaagcagc gtatcctcga cgatgcccga cagcgtgggc gacctgaagc cgcaatcaaa
     1801 gtgatgatcg aagaactcga ttcgccctgc acggaagaga tggccgcgtt cgataagttc
     1861 atcgactttg cgtcacaagc cgattggcag gcgatcgtgt tcgacaccgc gccgaccggt
     1921 cacaccatgc gcttgatcga actgccgatg gattggagtc agcagatcga tgtgaaggta
     1981 tttgcctcgg tcgataccac cgccgctgac gacgtcgcca agcagcgctt cggtgatgtg
     2041 atcaatatga tgcgcgattc ggcccgcagt accttcgcct ttgtcatgta tcccgaatcg
     2101 acgcccatct tagaagcgta tcgtgcggca gaagaactgc gcacagtcgg cattaagccg
     2161 ggcatggtcg tggccaactt cgtcattcca gctgagcgag ccaccacgcc gtttacgcag
     2221 tctcgacgtg tgatgcaaga aaaatatcta ggcgagatcg cacaacgctt cgcggtgccc
     2281 gtcgtgcaag tgccgctatt gccttatgaa gtgaaaggca tccccatgtt gatcgagttg
     2341 ggtcaacagg tttatgggct gggcgttgat gtcgaagcgg cacccctcgt tcatagtttg
     2401 ataaccgcag aatgatcgga gcaattacac atgacgaatc agtcccctgc ttatagtata
     2461 aacaactccg ccttgaatga attggaactg gcgtcgccgg aagtggtacg tcaatcggcg
     2521 cgtggttttg cggttgcgtt gggagagact ccgcagtatc aaaccttcga cgctgctgtg
     2581 cagcgattgt atgctgatga agttgcaatg gtggccttgg aagcctatca gactttgcag
     2641 cgatcactcc agacaatgtt catactcaac gccgtctccg atcatgatcg tgccgagctg
     2701 gagcgcctgc gacgggccta tgcggctcga cctgcggtta aagcctttgc gcaagcgcag
     2761 gccgagttga tcagtctgtg cca
//
LOCUS       DASRTJ010000718         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_213815,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000718 DASRTJ010000000
VERSION     DASRTJ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213815"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..412)
                     /locus_tag="VFF70_14520"
     CDS             complement(41..412)
                     /locus_tag="VFF70_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZY45949.1"
                     /translation="MKPETIRPIIQQAAEAWITGNADLFASFFTSDGEFVVPGKIHRG
                     REAIRQVTQSFADRYSDVKIDIRRIIIDGDQCVVEWYWEDTKKETGVRSRADDAIVVD
                     FKNGLICRWREYIDTKSPSVT"
     gene            complement(459..1277)
                     /locus_tag="VFF70_14525"
     CDS             complement(459..1277)
                     /locus_tag="VFF70_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZY45950.1"
                     /translation="MTDHPGEEITAGLVSASDAEWEGDQGDTFVAINAQLMQRYIYEY
                     KVKHSDFAPFPINAHHNAMNNPYAMMHYELTPEKFNGARMIADPINLLDCSPVCDGAA
                     AAILVPTEIARQFSPFPIRIVGSSIATDSLALHDRHNLLSLDAVALSAQKAYDQAKLK
                     PEDIDLFEVHDAFSITAVMSLEASGFAEAGKGWQLGRDGDIFLGGRLPICTMGGLKAR
                     GHPVGATGMYQIVEASMQLRGLAGKNQVKDAKIAMTQNIGGSGSNVVTHILERT"
     gene            1330..1530
                     /locus_tag="VFF70_14530"
     CDS             1330..1530
                     /locus_tag="VFF70_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45951.1"
                     /translation="MSGQCAGQHIANVYTVDPIDLCILDRGQHRIDCEEVDRLVPPIA
                     DRDLPDPNEAYIAHSIPLEISS"
     gene            1677..>2805
                     /locus_tag="VFF70_14535"
     CDS             1677..>2805
                     /locus_tag="VFF70_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45952.1"
                     /translation="MNDTSPQNLPRFGFAKFTLPPRPHDLLRRSRLLDFLHEHIQLRL
                     NLVCAAAGYGKTSLLIDFTHDTDYPVAWCRLDESDADWVVLVSDLLTAIRSPFPGFRS
                     MLPQLIGQTEPADLANVLIREIETNIDQYFILVMDDFHLVQSSPDVIRFVDALLANLP
                     EQAHLIISGRALPPLKMTALVAHQQVAGLSEEHLRFNTAEVQGLIQLRNDVAVSEAEA
                     EKIAANTEGWITGILLTAHLMWQGLIANLMRARQSEAPLYEYLADEVLNEQPEPFRRF
                     LMESAVLPEMEPSVCDAVLGRLDSADLLQQAETGRLFVSVVGNEFLTYQYHHLFREFL
                     ITKLNDQSPDRLKELRRRAANWYSDNAMPEAAVTFYVLAGDL"
BASE COUNT          622 a          740 c          733 g          710 t
ORIGIN      
        1 caggcacttc ggtcttcata tcgccccgct ggttgataaa ctaagttact gaaggtgact
       61 tcgtatcgat atactcacgc caacgacaga tcaagccatt tttgaaatcg acaacgatcg
      121 catcatcggc gcggctgcgc acacccgttt ccttcttcgt gtcttcccaa taccattcga
      181 cgacacattg atcgccatcg atgatgatgc gccggatgtc aatcttcaca tccgaatatc
      241 tatccgcgaa actctgtgtg acttgacgga tcgcttcgcg gcctcgatgg attttgccgg
      301 gcacgacgaa ttcaccatcg gacgtgaaga atgaggcgaa gagatcggcg ttgccggtga
      361 tccaagcttc agcggcttgt tggatgatgg ggcgtatcgt ttctggtttc atgttgatca
      421 aatcttacaa gcaatgaatt aaagactgaa attactcgtt aagttctttc caatatatgt
      481 gtcaccacgt tcgatccgct accgccgata ttttgcgtca tcgcgatctt agcatctttg
      541 acttgattct tgcccgccaa tccgcgcagt tgcatcgatg cttcgacgat ctgatacatg
      601 cccgtcgcac cgacgggatg gccgcgcgct ttcaggccac ccatcgtgca gatcggcaat
      661 cggccgccga ggaagatgtc accgtcgcgg ccaagctgcc agcctttgcc ggcttcagca
      721 aagccactcg cttcgagact catcaccgcc gtgatcgaga aggcatcgtg cacttcaaat
      781 agatcaatgt cttccggttt caacttcgct tgatcgtagg ccttctgcgc tgatagcgcg
      841 accgcatcga gcgagagtaa gttgtgtcga tcatgcagag ccaaactatc ggtggcgatc
      901 gaactgccca caatcctgat cgggaagggg ctgaattgcc gtgcgatctc ggtcgggact
      961 aagatcgctg cggcagcgcc atcgcacaca ggcgagcagt cgagtaaatt aatcggatcg
     1021 gcaatcatcc gcgctccatt gaatttttcg ggcgtcaact catagtgcat catggcgtaa
     1081 ggattgttca ttgcattgtg atgcgcgttg atcgggaagg gggcgaagtc cgaatgcttg
     1141 actttgtatt cgtagatgta acgctgcatc aactgtgcgt tgatggccac aaaagtgtcg
     1201 ccttgatcgc cttcccattc ggcatcggat gcgctgacga gtccggccgt gatctcttcg
     1261 ccgggatgat cggtcatctt ttcgacgccg caagccaact tgatcggcgg ctaatcccgc
     1321 gagcaaatct tgtccggcca atgtgctgga caacatattg ccaacgtata cactgtcgat
     1381 ccgatcgact tgtgcatcct ggatcgcggc caacatcgca tcgactgcga ggaagtagat
     1441 cggcttgtcc caccgatcgc cgatcgggat ctgcccgatc ccaatgaggc ttacatcgcg
     1501 catagcatcc ctctggaaat aagttcttga gtgtaggcag ctgcatctta tcacatttta
     1561 ttacaggagc gattcggtgg ttgaccttaa ccggattcgg gaatagaatc atttggttga
     1621 tcaaacagtg tgtagtatgc tcagacgaga agtattcaaa cgccatcaat tcggtcatga
     1681 acgacactag cccccaaaat ttaccccgat tcggctttgc gaaatttaca ctgccgccgc
     1741 gaccacacga tctactgcgc cgatcgcggc tgctggattt tctgcacgaa cacattcagc
     1801 ttcgattaaa tttggtgtgt gccgcagccg gttatggcaa gacttcgctg ctcatcgact
     1861 tcacgcacga cacagattat ccagtggcgt ggtgccgatt ggatgaatct gacgccgact
     1921 gggtcgtttt ggtatctgat ctactgacgg ctatccgttc gccgtttccc ggatttcgat
     1981 cgatgctgcc gcagctgatc gggcagacgg agccggctga tctcgccaac gttttaatcc
     2041 gcgaaattga aacgaacatc gatcaatact tcatcctggt catggatgat ttccatttag
     2101 ttcaatcgtc accggacgtg atccgctttg tggacgcgct gttggcgaat ttgccggagc
     2161 aggcgcacct gattatctcc gggcgtgcgt tgccgccatt gaagatgacg gcgttggtgg
     2221 ctcaccaaca agttgccggt ctgagcgagg agcatctgcg attcaacacc gcggaagtgc
     2281 agggcttgat ccaattacgc aatgatgttg ccgtctctga ggccgaggcc gaaaagattg
     2341 cagcgaatac tgaagggtgg atcaccggca ttcttttgac agcgcattta atgtggcagg
     2401 gtctcatcgc caatctgatg cgcgctcgtc aatctgaagc accgctgtac gaatatctag
     2461 ccgatgaagt tctcaatgaa cagcctgaac cattccgccg gttcttgatg gaatcggcag
     2521 ttctgcctga gatggagccg tccgtctgtg atgcggtctt ggggcgattg gacagcgccg
     2581 atttgttgca gcaagccgag acggggcgat tgttcgtcag cgtggtagga aatgaatttt
     2641 tgacctatca gtatcatcat ctcttccgcg aattcttgat cactaaatta aacgatcaat
     2701 ctccggaccg tctgaaggaa ttgcgccgtc gcgccgcaaa ctggtattct gacaatgcta
     2761 tgcccgaagc ggctgtaacg ttctatgtgt tggcgggtga tttga
//
LOCUS       DASRTJ010000719         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_146715,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000719 DASRTJ010000000
VERSION     DASRTJ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146715"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFF70_14540"
     CDS             complement(<1..730)
                     /locus_tag="VFF70_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZY45953.1"
                     /translation="MSATTTVNTTPTATSYQRFNIYHRLEHILALSSFVVLAVTGLPQ
                     KFAEQGWAQGLISFFGGIDTTRQIHHLAAIVLMMEVVYHIAAIGYRLIVRRVRPSMLP
                     TIRDASDAVGTLLFNVGLRKQRPQGGRYTFEEKVEYWAFVWGTVVMVITGFMLWNPIA
                     TTSILPGEFIPAAKTAHGSEALLAVLAIIIWHMYSVHVRRFNKSMWTGKLTEHEMLEE
                     HPLELADIKSGLAVRPIAPIDLKKR"
     gene            complement(727..1848)
                     /locus_tag="VFF70_14545"
     CDS             complement(727..1848)
                     /locus_tag="VFF70_14545"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZY45954.1"
                     /translation="MKSWLRWVGLGMLAGLFLFGWAAINVQAAPVTQTPTPQAITSDQ
                     QCNDCHTKADQYKPLLSGESLYLTVDQTAFDISLHGQNEVSCSSCHSNITTYPHPPLV
                     QQSLRQIAASFSQSCKECHADEAARQLDSIHQQARAQGNENAAVCSDCHNPHYTTQPA
                     EPRSKIAVTTCNKCHSGIAQEYRQSVHGAALVGDENPDVPVCIDCHGVHNISDPRTNQ
                     FLLNSPQICAKCHSDPQRMSKYGIRTDVVNTYVADFHGTTVTLFEQKSPDQLPNKPLC
                     IDCHGIHDIKSIKDPDSPVVKANLLATCQKCHPSATDNFPGAWLSHYPPSPDHNPLVY
                     YVGVFYQIFIPLVLGGLLLFVVTDMGHRLVRRIKGGPRK"
     gene            2393..>3706
                     /locus_tag="VFF70_14550"
     CDS             2393..>3706
                     /locus_tag="VFF70_14550"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZY45955.1"
                     /translation="MKRTPLFIAVVLLAIGLALVACSSTPAPTAAPTTPPNTAPQPTA
                     APAAPLIEVPFLTGWQGSAHADAKAEAFNHWNTATPAEVPVDCARCHSTTGYQDFLGA
                     DGSAAFAVDKAQPIGQVITCVACHNTATANLTSVTFPSGLKIEGLGPEARCMQCHQGR
                     ASGATVDAAIKKAALTDDDTVSPDLGFTNIHYLAAGAMLYGSEAAGGYQYTDQLYDMK
                     FRHVPGKDTCLGCHNQHTLEVKVDECTACHKDAKTKDDLKNIRMNGSLADYNGNGDVK
                     EGIAAELDGLRTTLYSTIQAYAKDVSKTPIVYDTATYPYFFIDTNGNGQSDKDELVAA
                     NAFKAWTGRLAKAAYNYQTSIKDPGFFAHNAKYAIELMYDSIADLSTKLSAKIDMTKM
                     ARNDSGHFAGINMPFRDWDAEGEVPFRCAKCHTSDGLPTFVAAAGT"
BASE COUNT          883 a         1031 c          977 g          815 t
ORIGIN      
        1 gacgcttctt tagatcgatc ggagcgatcg ggcgcaccgc taaacccgat ttgatatcgg
       61 ccaactccaa tggatgctct tcgagcattt cgtgttcggt cagcttgccc gtccacatac
      121 ttttattgaa gcggcgcaca tgcacgctgt acatatgcca gatgatgata gccagcacgg
      181 ccaacagcgc ttcgctgcca tgcgcggtct tcgcggctgg aataaattcg ccgggcaaga
      241 tcgaggtggt ggcgatgggg ttccacagca tgaagccggt aatgaccatg acgacggtgc
      301 cccacacgaa cgcccagtat tccacttttt cctcgaaggt gtagcggccg ccctgcggcc
      361 tttgtttgcg cagccctacg ttaaacagca gcgtaccgac ggcatcgctc gcgtcacgga
      421 tcgtcgggag catgctgggg cgcacacgtc gaacgatcag gcgataaccg atcgcggcga
      481 tatgatacac cacttccatc atcaacacga tcgccgcaag gtgatgaatc tgccgcgtcg
      541 tatcgatacc gccgaagaaa ctgatcaagc cctgcgccca accctgttcg gcaaactttt
      601 ggggcaagcc tgttaccgct aacaccacaa aggaactcaa cgcgaggata tgctcgaggc
      661 gatggtagat attgaagcgc tgatagcttg tggctgtcgg ggttgtattc acggtcgttg
      721 ttgcgctcat tttcgggggc ctcccttaat tcggcgaacg agccggtgac ccatatcagt
      781 cacgacgaac aacaataaac cgcctagcac cagcgggata aagatctgat agaacacgcc
      841 gacgtagtaa accaacggat tgtgatccgg tgacggcggg taatggctca accacgcacc
      901 cgggaaatta tcggtcgcgg agggatggca cttctggcag gtcgccaata gattggcttt
      961 gacgaccggc gagtccgggt ctttgatcga cttgatgtca tggataccgt gacaatcaat
     1021 gcacaacggt ttgttcggca attgatcggg cgacttctgt tcaaacaagg tcaccgtcgt
     1081 gccgtgaaaa tctgcgacat aggtattgac cacatcggtg cggatgccgt acttcgacat
     1141 gcgctgcgga tcagagtggc acttggcgca aatctgcggc gagttcaaca agaattgatt
     1201 ggtgcgcgga tcagaaatgt tgtgtacgcc atgacaatca atgcaaaccg gcacatccgg
     1261 attttcatcg ccgactaacg ccgcgccgtg cacactctgt cgatattcct gtgcgatgcc
     1321 gctgtggcat ttattgcagg tggtgacggc aatcttcgat cgcggttcgg caggctgggt
     1381 agtgtaatgc gggttgtggc agtccgagca cactgcggca ttttcattgc cctgcgcgcg
     1441 cgcctgctga tggatactat ccaactgccg tgcggcttca tcggcgtggc attctttaca
     1501 cgattgcgaa aaactcgcgg cgatctgtcg caagctttgc tgcaccaacg gcggatgcgg
     1561 atacgtggtg atgttcgagt gacagctgct gcagctcact tcattctggc cgtgcagcga
     1621 aatgtcgaag gctgtttggt cgaccgtcag ataaagtgat tcaccactca agagcggctt
     1681 atactgatcc gctttggtgt ggcagtcatt gcattgttga tccgaggtaa tcgcctgcgg
     1741 cgtcggcgtc tgagtgactg gcgcggcttg cacgtttata gccgcccagc caaacaaaaa
     1801 caaaccggcc aacataccca gccccaccca acgcaaccaa gatttcatgc cgatccccgt
     1861 gatataataa agtagatgga attctagacg tctagtattt tacatgatat atgcatatgc
     1921 gaatagtcca cagggacaac tgtcactgcc ttatgtgatg aacatcattc gtgaaatgtt
     1981 ttgcctgtat tcgatagccc gaaggtcagc atgcaaacga acgaccgatc ggccagctca
     2041 acccgatcgg tttttcttca gccgcaaagc tatacaaact gcggccaaga ttgagacaac
     2101 tacagtaatt ttactcgtac attcatcact tattctaggt gatgattatt actagccata
     2161 tgttcaatca ttcacataat agagtagaac ctatataatc atacagctga aatgccgtgt
     2221 cgaaagcacg ggagttaatc gcatattttt agcgcctttt gcagtgaaaa taaatcttgg
     2281 gcgaatttga tcgggttgcg ttgtgtcatc ggcgtcacaa tcgaccggat tgatttttca
     2341 atcgtatgaa gttacaatac tcactctcaa ctctaaaagg agaagaccac atatgaaacg
     2401 aacgccgcta ttcatcgcag tagtgttgtt ggcgatcgga cttgcgttgg tagcctgtag
     2461 ttcaacgcct gccccgaccg cggcgccaac cacaccgcct aatactgcac ctcagccgac
     2521 cgccgcaccg gctgccccgt tgatcgaagt tccgttcctg actggttggc aaggctccgc
     2581 gcacgccgac gcaaaagccg aagccttcaa tcactggaac acggccacac cggctgaagt
     2641 gccggttgat tgcgcgcgct gccacagcac caccggctac caagatttct tgggcgcgga
     2701 tggctcggcg gcttttgctg tcgacaaagc tcagccgatc ggccaagtga tcacctgcgt
     2761 ggcctgccac aacaccgcca ctgccaatct gacgagtgtc actttcccat caggcctcaa
     2821 gattgaaggc ctcggccccg aagcacgctg catgcagtgc catcagggcc gcgcttccgg
     2881 tgctacagta gacgccgcga tcaagaaagc ggccttgact gacgatgata ccgtcagccc
     2941 cgatctgggc ttcaccaaca tccactatct ggcagctggc gccatgctgt atggcagtga
     3001 ggccgctggt ggttatcaat ataccgacca gctgtatgac atgaagttcc gccacgtgcc
     3061 cggcaaggac acctgcctgg gctgccacaa ccagcacacg ctggaagtga aggtggatga
     3121 gtgcaccgcc tgccacaaag atgccaagac caaagacgat ctcaagaaca tccgcatgaa
     3181 cggatcgttg gcggactaca acggcaacgg cgatgtgaag gaaggcattg cggccgaact
     3241 tgacggactg cgcacgacac tgtacagcac gatccaggcg tatgccaaag acgtctccaa
     3301 gacgccgatc gtctatgaca ccgccaccta tccgtacttc ttcatcgata cgaatggcaa
     3361 tggccagagc gacaaagatg aattggttgc ggcgaatgcg ttcaaagcct ggaccggtcg
     3421 tttagccaaa gcggcttaca actatcagac ctcgatcaaa gatccaggct tctttgcaca
     3481 caatgccaag tacgccattg agttgatgta tgactcgatc gcggatctca gcaccaagtt
     3541 gagcgccaag atcgatatga ccaagatggc ccgcaatgac tccggtcact tcgccggaat
     3601 caatatgccg ttccgcgatt gggacgccga aggcgaagtt ccgttccgct gcgccaagtg
     3661 ccacacctct gatggtctgc ccacgttcgt cgcagctgct ggcacg
//
LOCUS       DASRTJ010000720         8067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_146728,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000720 DASRTJ010000000
VERSION     DASRTJ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146728"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3122)
                     /locus_tag="VFF70_14555"
     CDS             complement(<1..3122)
                     /locus_tag="VFF70_14555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZY45956.1"
                     /translation="MSFVHLHVHSEYSVLDGLSKVSAIVNRAKELNQTAVALTDHGVM
                     FGVIDFFNAATKAGIKPIVGMEGYLARRSRLDRDPQKDKSPFHLLLLAQNMTGYQNLL
                     CLASAAQLEGFYYRPRVDKEILEKYSEGLIVTSGCGSAEVPRAILGGQLDQARQALSW
                     YVDVFGRERFFIELQQHEGFPELLTLNRQLIQFAKEFNLKVVATNDAHYIKQEDSRAQ
                     DVILCIGTGSLMSQTDRMKMTDTSYYLKSSDEMAAMFSELPEALTSTLDIAEMCEVDL
                     SSKGYHLPIFELPIGVTAADRLRQLCEAGLVRRYPDRLNDPIVRQRLEYELSVINKMG
                     FDTYFLIVADLTRYAQEHDIWWNVRGSGASSIVAYTTNITNLDPLPHNLIFERFLNPG
                     RISMPDIDLDFPDDRRAEMIDYTVRKYGKDKVAQIVTFGTLGARAAIRDVGRALDIPL
                     GDVDRIAKLVPNIPGKPMTLHEAIEQIPDLKQLYESTDYIRELIDTAISVEGTVRNVG
                     THAAGVVISDRPLIEYTPLHRPTKSDEASPLESITQFEMGVVESIGLLKVDFLGLITL
                     SIMRKACDLIKANHGVDLNLTNIPTNDPDAFALMSRGEVLGLFQVEGSGMRRMLMDMR
                     PTKFENIVAAISLFRPGPMEYIPMYIKRMHGEEPIKYHHPSIEPILAETFGIIVYQEQ
                     IIQIAVTLAGYAPGEADEIRKAVGKKIEEKIKAHRAKFIQGAINNHIDQQVAEAVYDD
                     IEYFARYGFNKAHAADYALLTCQTAYLKSYYPVEYMTALMTCEKSVEKIGLLIVECKR
                     MGIDVLRPDVNHSDLDFTIEASADGKRSIRFGMASIKNVGEGPVKAIREARLVGGSFK
                     SLEDFARRVDLRVVNRRALEALIKVGAFEDFGNRAQLLQILDRLMGLSSGAHRAKDVG
                     QISLFGAQMDEGDGAIGKLPKIEEAPMREKLAWEKELIGAYVSEHPVARALAELQDQI
                     THLSGELNEDLNQQKVTMVGSVVTSRTIMTKKNEPMGFITLEDVQGTFECVAFPKTWK
                     QTQALWQKDK"
     gene            3301..4119
                     /gene="mutM"
                     /locus_tag="VFF70_14560"
     CDS             3301..4119
                     /gene="mutM"
                     /locus_tag="VFF70_14560"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZY45957.1"
                     /translation="MPELPEVETIVRRLRQPLIGRTITKVTYDWPRTISRPAPKEFAR
                     RMKGLTFQSIDRRAKYLVFTVGQDDNLSYILIHLKMSGRLNIVLKAEPISKHDRVIFD
                     LDNGQQLRFNDVRKFGRVHLVADPAEITGKHGPEPLDSTFTLKKFREMIQSRSGAIKP
                     LLLNQNFLAGVGNIYADEALWSAHIHPLRKVDSLKDAEVAALHRSIRKILNRAIDDQG
                     TDAGDGVIEAGDYTPRVYGREGRPCKRCGYIIHKMWVGQRGTHYCPHCQAKRGK"
     gene            4120..4269
                     /locus_tag="VFF70_14565"
     CDS             4120..4269
                     /locus_tag="VFF70_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45958.1"
                     /translation="MPFNQFLILVAVFGVALLLIIVGLIVTTHNRSAAKASHDAVTSS
                     KPAPG"
     gene            4279..4818
                     /locus_tag="VFF70_14570"
     CDS             4279..4818
                     /locus_tag="VFF70_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45959.1"
                     /translation="MAGAAGKTITGDAPLTTAQLDAIFVTRDPASNEWVVDVNGTRYR
                     NLKDIHDDLTAHKVLDALSGLQRFAGSIPIVKAPALIVDPLSTGSRAAVSPTSSAGSS
                     SSVPSPKDSLLDQIDAVLQHNLLRHAELSTRVIHMGAAADGSLRIEVDRQFYTAPDEI
                     PERSIREIIKLSIQEWERS"
     gene            complement(5012..6019)
                     /gene="add"
                     /locus_tag="VFF70_14575"
     CDS             complement(5012..6019)
                     /gene="add"
                     /locus_tag="VFF70_14575"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZY45960.1"
                     /translation="MPKVDLHRHLEGSLRLETLAEIAREHGIDLPSYEIEHLRPYVQI
                     TNDPPNFQRFLEKFSLLRRFYTTREAVERVAYEAVVDAAEDNVRYLELRFNPAALSQA
                     GGFGFDEVADWVIEAADRAAAETHIIVRLLCTIVRHEPIELAERTIEVALARRDRGIV
                     GVDLAGDEVRYSALPFRDLLRRAADGGLGITIHAGEAGGADNVREAIELLGANRIGHG
                     IRSIEDLDVIKVLRDHHVTLEICPTSNLQTGVMQKFGHHPLRDLYLLGVPVTINTDDP
                     SISDTTLTDEYLVAIMGMGVTLQDVREFNRNAIQAAFISADERAKLEQRLLAEFDQHL
                     K"
     gene            complement(6106..>8067)
                     /locus_tag="VFF70_14580"
     CDS             complement(6106..>8067)
                     /locus_tag="VFF70_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45961.1"
                     /translation="AEELASVLRVPMTSRGQMIGMLDIYGKSKPRRFGEDEVELAGIF
                     ASQVAVAIENAWLYDETSRRLDEITILFEVARAGASTLDLNQVLDRMLDAVRRTLRFD
                     TFEFLLYDSVSHILHTRAAYGFPPETAQAAIRLGEGIVGRVAQTRQPVLVNDVSREPN
                     YILGQPNTRAELAVPMQVADQIVGVMNVESMRPNAFTHDDERLLSALAGQLAVIIENA
                     RLYEETQHGLAEVSTLYSFAQKLTTSLELDELLDSIVSTLKELLDCRGVSIALLMPES
                     QLLEIRAAAGLQAKWQQAAKLKVGEGISGKVAETAQSIYVPDTRAMPDFIFFDPIVRS
                     LLVVPLRVKDRVIGTLAIDHVKADAFTKNDERLLAIAAAQAAIVIENAQLYADIKERA
                     SKLEQAYLELQQIDKLKDELVQNVSHELRTPLTFIKGYVELLLESDMGPLNENQRESL
                     TIVAEKTNALTRLVSDIIFLQQIERESLELATLDLNEIGRMALQSCEVTATSAGISLR
                     LDAKPDMVKVPVDRDRINQVFDNLLGNAIKFSPKGGTIAITVEDIGNALKITVADDGI
                     GIPADKLPHIFDRFYQVDGSATRRFGGAGLGLAITKRIVEAHGGHIWAESKFGAGSQF
                     IFTLPKDQPGVNEPLDVARATALNLDESN"
BASE COUNT         1836 a         2297 c         2091 g         1843 t
ORIGIN      
        1 atcttatctt tctgccagag cgcttgtgtt tgtttccagg ttttcggaaa cgccacacat
       61 tcaaaagttc cctgcacatc ttcgagcgtg atgaaaccca tcggttcatt cttcttggtc
      121 atgatcgtac gcgaagtgac caccgagccg accatcgtta ctttttgctg attaaggtct
      181 tcattcaact cgccgctcaa gtgtgtgatt tgatcttgca gctccgctaa ggctcgagcc
      241 accggatgct cggacacata cgccccgatc aattcttttt cccaagccaa cttttcgcgc
      301 atgggcgctt cttcgatctt aggcagcttg ccgatggcgc cgtcgccttc gtccatctgt
      361 gcgccgaaca aggagatttg gccgacatct ttagcgcgat gcgcgcccga actcaaaccc
      421 atcaatcgat cgagaatttg caacaactgc gcgcgattgc caaaatcttc aaacgcgccg
      481 actttgatca acgcttcgag cgcgcggcga ttgaccacgc gcagatcgac gcggcgcgca
      541 aaatcttcca ggctcttgaa gctgccgccg accaatcggg cttcccggat cgctttaacc
      601 ggcccttcgc ccacattctt gatcgacgcc atgccaaagc ggatcgaacg tttgccgtcg
      661 gctgaagctt cgatcgtgaa atcaagatcg ctgtgattca catccggccg cagcacatcg
      721 atgcccatgc gcttgcattc gacaatcaat aagccgatct tttcaacgct cttctcgcag
      781 gtcatcaacg cggtcatata ctcgaccgga taatacgatt taagataagc ggtctggcag
      841 gtcagcagcg cgtagtcagc ggcgtgcgct ttgttgaaac cgtaacgtgc aaaatattcg
      901 atgtcgtcgt aaacggcctc ggccacttgc tgatcgatgt ggttgttaat cgcgccctga
      961 ataaatttgg cgcgatgcgc tttgatcttt tcttcgatct tcttgccgac agctttgcga
     1021 atctcatccg cttcaccggg cgcgtaaccg gccaacgtca cggcgatctg aatgatctgt
     1081 tcttgatata cgataatgcc gaaggtctcg gccaagatcg gttcgatgct gggatgatga
     1141 tatttgatcg gttcttcgcc gtgcatgcgc ttgatgtaca tcggaatgta ttccatcggg
     1201 ccggggcgga acagcgaaat ggcggccacg atgttttcga atttcgtcgg gcgcatgtcc
     1261 atcaacattc ggcgcatgcc gctgccttca acttggaaca gacccagcac ttcaccgcgt
     1321 gacatcaacg cgaacgcgtc aggatcgtta gtaggaatgt tggtgagatt cagatcaacg
     1381 ccatgattcg ctttgatcag atcacaggct ttgcgcatga tgctgagcgt gatcaaaccg
     1441 aggaagtcga ctttcaacaa gccgatcgat tcgacgacgc ccatctcgaa ctgcgtgatc
     1501 gattcaagcg ggctggcttc atcggacttg gtcggtcgat gcaacggcgt gtattcgatc
     1561 agtggccgat cggagatcac gacgcctgcg gcgtgggtgc caacgttgcg caccgtgcct
     1621 tcaacactga tcgcagtgtc gatcaactcg cggatgtaat cggttgattc atatagttgc
     1681 ttgagatcgg gaatctgctc aatcgcttcg tgcagcgtca tcggtttacc gggaatattc
     1741 ggcacgagtt tggcaatgcg atcgacatcg cccagcggga tatcgagcgc gcggcccacg
     1801 tcgcggatcg cggcgcgcgc gcctagcgtg ccgaaggtga cgatctgcgc gactttatct
     1861 ttgccatatt tgcgcacggt gtaatcgatc atctcggcgc gccgatcatc gggaaagtcg
     1921 agatcgatat cgggcatgct gatgcggccc gggttgagga aacgttcgaa gatcaaattg
     1981 tgcggcagtg gatcgagatt ggtgatgtta gtggtataag ccacgatcga actggcgccc
     2041 gagccgcgca cgttccacca aatatcgtgc tcttgtgcat agcgtgtgag atcggcgacg
     2101 atcaagaagt aggtatcgaa gcccatcttg ttgatcacgc tcaactcata ctctaaacgc
     2161 tgacgaacga tcggatcatt caaccgatct ggatagcgcc gcaccaaacc cgcttcgcac
     2221 aattggcgta atcgatcggc cgccgttacc ccgatcggca attcgaagat cggcagatga
     2281 tagcccttcg acgacagatc gacttcgcac atctcggcga tgtcgagcgt ggaggtcaac
     2341 gcttcaggaa gttcactaaa catcgccgcc atctcgtcgc tgcttttcaa gtagtacgag
     2401 gtgtcggtca tcttcatgcg atcggtctgg ctcatcagcg agccggtgcc gatgcacaag
     2461 atcacatcct gcgcgcggga atcctcttgc ttgatgtagt gcgcgtcgtt ggtcgcgacg
     2521 actttcaagt tgaactcttt ggcgaattga atcaactgcc gattgagcgt cagcaattca
     2581 ggaaagccct cgtgctgttg caattcgatg aaaaagcgct cgcggccaaa gacatcgacg
     2641 taccagctca aggcctgccg cgcttgatcg agttgaccgc cgagaatggc gcgcggcact
     2701 tcggccgagc cgcagcctga ggtgacgatc aaaccttcag aatatttttc cagaatttct
     2761 ttatcgacgc gtgggcggta atagaaacct tccaactgcg cggcgctggc gaggcacaat
     2821 aagttttgat agcccgtcat gttttgcgcg agcagcagca aatgaaacgg acttttatct
     2881 ttctgcggat cgcgatcgag gcgcgagcgg cgggcgaggt aaccttccat accaacgatc
     2941 ggtttgatgc cggccttcgt ggccgcgttg aagaaatcga tcacgccgaa catcacgccg
     3001 tgatcggtta aggcgacagc ggtctgattc agttccttgg cgcgattaac gatcgccgag
     3061 actttgctga ggccgtcaag caccgagtat tcgctatgga cgtgtaagtg aacgaaagac
     3121 atttaattcg gcctcatatg atcgaggaga ctgcgaagtt tgcttcgcgc tcgattatat
     3181 cttacattca aggtgcgtca aggtatcaac gaatggcaac gaatgatcga atcagcgcgc
     3241 aatgatataa ttgacgcgct ctaagatcag aaatcaaaaa tcagcgatca gagattcccg
     3301 ttgcctgaac tccccgaagt cgaaaccatc gtccgccgat tgcgccagcc gctgatcggt
     3361 cggacgatta cgaaagtcac ctacgattgg ccgcgcacga tcagccgacc ggcgccgaaa
     3421 gaatttgcgc gccggatgaa agggctgaca tttcagtcga tcgatcgacg cgcgaagtat
     3481 ttggtgttca ccgtcggaca agatgataat ttgtcttaca ttctcatcca cttaaagatg
     3541 agtggccgct tgaatatcgt gttgaaggct gagccgatca gcaaacacga tcgggtgatc
     3601 ttcgatctgg acaacgggca acaactgcgt ttcaacgacg tccgaaagtt cggccgcgtg
     3661 catctggtcg ccgatccagc agagatcacg ggcaagcacg ggccggaacc tttggattca
     3721 acctttactt tgaagaagtt ccgtgagatg atacaatcgc ggtcgggcgc gatcaaaccg
     3781 ctgctgctca atcaaaattt tctggcgggc gtcggcaata tctatgcgga tgaagcgctg
     3841 tggtcggcac acattcatcc gctgcgcaaa gtcgattcgc tgaaagacgc cgaagtcgcg
     3901 gccctgcata gatcgatccg caaaatatta aatcgcgcta tcgacgatca aggcactgat
     3961 gcgggtgacg gcgtgatcga ggcgggtgat tacacaccgc gcgtatacgg gcgcgaaggc
     4021 cgaccctgta aacgctgcgg gtatatcatt cacaagatgt gggtcggtca gcgcggcaca
     4081 cattattgtc cacactgtca agcaaagcga ggcaagtaaa tgccattcaa tcaattcctg
     4141 attttggtcg cagtcttcgg cgtcgcactg ctcctcatta ttgtcggatt gatcgtgacc
     4201 acgcataatc gatcggcggc gaaagcctcg catgacgcgg tcaccagctc caagcccgcc
     4261 ccgggttaga taaaaaattt ggcgggcgcg gctggcaaga ccatcaccgg tgatgcgccc
     4321 ttgaccacgg ctcaattaga tgcgatcttc gttacgcgcg atccggcctc gaacgaatgg
     4381 gtggtcgatg tcaatggcac ccgctatcgc aatctaaaag atattcacga cgatctgacc
     4441 gcgcataaag tgctggacgc gctcagcggc ttgcagcgct ttgctggatc gatccccatc
     4501 gtgaaggctc cggcgctgat cgttgatcca ttgtcaaccg gctcacgggc agcggtcagc
     4561 ccaacatcaa gcgcaggatc cagctcgagt gttccctcac caaaggacag tctgctcgat
     4621 cagatcgatg cagtcctgca gcataatctc ctgcggcatg ctgagctctc gacgcgagtg
     4681 attcatatgg gtgcagccgc ggatggcagt ttacgcatcg aagttgaccg gcagttctac
     4741 actgcgccgg acgaaatccc cgaacgatcc attcgcgaga tcatcaaatt atccattcag
     4801 gaatgggaac gctcctagat cgttatccaa atttattgat ggcgttacgc ttcacagcac
     4861 cacgctcacg ctcttcgagc acgtcttgtc tttcacgccg ggttcccggc cgcgcggtcg
     4921 ttgagcaaag tatcagaacg ctcttaaaca aaactccggc ctgatattca tggccggagt
     4981 tttcagctac tcaacaattg ccaagcactg actacttcaa gtgctgatca aattctgcca
     5041 gcaaacgctg ctctaatttt gcgcgctcat cggccgagat gaaggctgcc tggatcgcgt
     5101 tccgattaaa ttcccgaaca tcctgcagcg tgacgcccat gcccattatc gctaccaaat
     5161 attcatcggt caaggtggtg tcggaaatgc tgggatcgtc cgtgttgatc gtgaccggca
     5221 cgcccagcag ataaagatcg cgcagtggat ggtgtccaaa tttttgcatg acgccggtct
     5281 gcaagttgct ggtcgggcag atctccagcg ttacatggtg atcacgcagc accttgatca
     5341 catctaaatc ctcgatcgat cgaataccgt ggccgatccg gttcgcgccg agcagctcga
     5401 tcgcttcccg aacgttatcg gctccgccgg cttcaccggc gtggatagtg atgcccaaac
     5461 cgccatcagc cgcgcggcgc aacagatcac gaaacggcag cgccgagtaa cgcacttcat
     5521 cgccggccag atccacgccg acaatgccgc gatcgcgccg ggccagcgcc acctcgatcg
     5581 tgcgctcggc cagttcgata ggttcgtgcc ggacaatcgt gcacaacaac cgcactatga
     5641 tatgtgtttc agcggccgca cgatccgcag cttcgatgac ccaatcggcc acctcatcaa
     5701 atccaaagcc tccggcctgc gacagcgctg ccggattaaa gcgcaattcc aaataacgca
     5761 cgttatcctc ggccgcatcg acaacagctt catacgccac gcgctccact gcctcgcgcg
     5821 tcgtatagaa gcggcgcagc aaactaaatt tttccaggaa gcgctggaag ttcggcggat
     5881 cgttggtgat ctgcacgtac ggccgcagat gctcaatttc ataactgggc agatcgatgc
     5941 catgctcgcg cgcgatctcg gccagcgttt ccaagcgcag cgagccttcc aaatgccggt
     6001 gcagatcgac tttaggcacg cgctgaagta ttcgacgcag ctccggaatg ctgcgcgcga
     6061 tcgatcgggt aaatgagggt tcagtgttcg tgttggccag tatgatcagt tagactcatc
     6121 tagatttaag gccgttgcgc gcgccacatc tagcggttca ttcacacccg gctggtcttt
     6181 gggcagagta aaaatgaatt ggctacctgc accgaatttg ctctcggccc aaatatgacc
     6241 gccatgtgcc tcgacgatgc gcttggtgat cgccagaccc aggcctgcgc cgccgaaccg
     6301 ccgcgtagcc gatccatcca cttgataaaa gcgatcgaag atgtgcggca gcttgtcggc
     6361 tggaatgccg atgccatcat ccgcgactgt aattttcaaa gcgttgccaa tatcttcgac
     6421 tgtgatcgca atggtcccgc ccttcggact gaatttaatc gcattgccca gcaaattatc
     6481 aaagacttgg ttgattcgat cccgatcgac cggcaccttg accatatccg gctttgcatc
     6541 caaccgcagt gaaattccgg cgctggtcgc cgtcacctca caactctgca aagccatgcg
     6601 cccgatctcg ttcaaatcca acgtagccag ttccagtgac tcccgctcga tttgctgcaa
     6661 gaagatgata tcgctgacca aacgcgtgag ggcattcgtc ttctcggcca caatcgtcaa
     6721 actctcgcgc tgattctcat tcagcggtcc catgtctgat tccaacaaca gctcgacgta
     6781 gcctttgatg aaggtcagcg gcgtgcgcaa ttcgtgcgat acattctgca ccagttcatc
     6841 cttcagttta tcgatctgct gtaattccag ataggcttgc tccagcttgc tagcgcgctc
     6901 cttgatgtca gcatacaact gcgcattctc gatgacgata gcggcttgcg ctgcagcaat
     6961 cgccaacaaa cgctcatcgt ttttcgtaaa ggcatccgcc ttaacatgat cgatcgccaa
     7021 tgtgccgatg acccgatctt ttaccctcag cggcacgacg agcagcgaac gcacaatggg
     7081 atcgaaaaag atgaaatccg gcatcgcgcg agtgtccggc acatagatcg attgagcggt
     7141 ttccgctacc ttgccgctga tgccctcgcc gacttttaac ttcgcggctt gttgccattt
     7201 tgcttgcagt ccggccgcag cgcgtatttc caatagttga gattcgggca ttagcagtgc
     7261 aatgctaacg ccacggcaat ctaacaattc tttcagtgtg ctgacgatcg aatccagcaa
     7321 ttcatccagt tcgagactgg tcgtcaattt ttgcgcgaat gaatataagg tcgaaacttc
     7381 ggccagccca tgttgagttt cttcgtacag gcgcgcattc tcgatgatca cggcaagctg
     7441 cccggccaac gcacttaaca ggcgctcatc atcatgcgtg aacgcattcg ggcgcatgct
     7501 ctcaacattc atcacgccga cgatctgatc ggcgacttgc atcggcacgg ccaattcagc
     7561 ccgcgtgtta ggctggccca aaatatagtt tggctcgcga ctgacatcgt tgaccagcac
     7621 cggctgccgc gtttgagcca cacgaccaac gataccttcg cctaagcgaa tggcggcttg
     7681 cgcagtttcg ggcgggaagc cgtacgcagc ccgcgtgtgc aatatatggc tgactgaatc
     7741 gtaaagcaaa aattcaaacg tatcaaagcg cagcgtccgc cgcaccgcat ccaacattcg
     7801 atcaagcacc tgattcagat ccaaggtcga tgcaccggcg cgcgcgactt caaacagaat
     7861 cgtaatttcg tccaatcgcc ggctggtttc atcataaagc cacgcatttt cgatagccac
     7921 ggcaacctgg ctggcaaaga ttccggccaa ttcaacctca tcttcaccaa aacgccgcgg
     7981 cttggacttg ccgtaaatat ctaacatgcc gatcatttgg ccgcggctgg tcatcggcac
     8041 acgcagcaca ctggccagct cttcagc
//
LOCUS       DASRTJ010000721         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_374850,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000721 DASRTJ010000000
VERSION     DASRTJ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374850"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VFF70_14585"
     CDS             complement(<1..1526)
                     /locus_tag="VFF70_14585"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HZY45962.1"
                     /translation="MKQTYQIMLPITQVDRAEVLLPLAMAWLHQMVAASKGMKGEIVL
                     IGAVCVPKNQNLSVGAQNAQGLRAAMNSIRNRYTDLPLRVKAAVQVSYEPWQEVLQAI
                     QAEEPDLILLDWNGDPAASIMGVPLAEVLSETPCELALVRPTDWNKIDRILLPIRGGP
                     YTKLAVNFSLAAAEMANAEITVMYAGSRGHIDQPYASYLPLLRNIDRIKRHLTIVGDL
                     VDEVLSQAEEHELIVMGASSRPGKIGPEPLGPIASRIATESSIGLVLVKSPRAFTPIS
                     TRPSSVPSSVPVLVDKWFAENTFDASEFDDIDRLIDLKRERNVSISLALPALNEEATV
                     GKVIRTIRRALMKNHSLLDEIVLIDSGSDDRTREIAQRLGVPVYIHQEILPQYGAHTG
                     KGEALWKSLHVTQGDLIAWIDTDIVNIHPRFVYGVIGPLLHSDRIQFVKGFYRRPLKV
                     GDKMQAGGGGRVTELVARPLINLFFPELSGLIQPLSGEYAGWRSALEQVPFFTGYGVE
                     T"
     gene            complement(1526..>2749)
                     /locus_tag="VFF70_14590"
     CDS             complement(1526..>2749)
                     /locus_tag="VFF70_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZY45963.1"
                     /translation="MLRHAQLMIDAGYEVSIITGSGAQFDDRIPVQVVPEIGSRNADV
                     LKVKQQLDAGQVTSDFEQLRNRILETLRSLLTDVDVLIAHNVFTLHKNLPLTTALRNF
                     IDTSPPASAKRPRSEAETGRPLRVIAWHHDLAWTNELYRSQLHDGYPWNLLREAWPNV
                     QNVTVSKSRQVELAKLYQIDPNSIEVIPPGVDPANFFRWTSLTRELVAKLDLLAADLI
                     LLLPARITQRKNIELGIRVLAELRKQTQLDARLIVSGPPGPHNPMNDAYLQSLLNLRR
                     ELKIDRAAHFLYECGQSDQLLIPDETLADLYTLADAVFFPSQQEGFGIPILEAGLARL
                     PIFCADIPALRETGQADAHYFAPDADPIELAKLIGRELITNGAHRLQKRVRSEYFWQR
                     IFADRIEPLLKKRAE"
BASE COUNT          611 a          772 c          748 g          618 t
ORIGIN      
        1 gtctctacgc catagccggt aaagaaaggc acctgttcca acgcgctgcg ccagcctgcg
       61 tattcacccg ataacggttg aatcaagccc gacaattccg ggaagaacaa attgatcaaa
      121 ggccgcgcga cgagttctgt cacccgtccg ccgcccccgg cctgcatctt gtcaccgact
      181 ttgagcgggc gtcgataaaa gcctttaacg aattgaatgc gatcggagtg cagcagcggt
      241 ccgatgacgc catatacaaa acgcggatga atgttgacga tgtccgtatc gatccaggca
      301 atgagatcgc cctgggtcac gtgcaagctt ttccacaacg cttcaccttt gccggtgtgt
      361 gcgccgtatt gcggtaaaat ttcttgatgg atatagacgg gtacacccaa gcgctgcgcg
      421 atctcgcgcg tccgatcgtc cgaaccggaa tcgatcaaga cgatttcatc gagcagtgaa
      481 tgatttttca tcaatgcacg tcgaatcgtg cggatcactt tgcccaccgt ggcttcttca
      541 ttcaatgcgg gcagcgccaa gctgatcgat acattgcgct cgcgtttgag atcgatcagc
      601 cgatcgatgt cgtcgaactc gctggcatcg aaggtgtttt cggcgaacca cttatcgacc
      661 agcactggta ctgaactggg cacactgctg ggccgggtcg agatcggcgt gaaggcgcgc
      721 ggcgatttga ccagcaccaa cccgatcgag gattcggtcg caatccggct ggcgatcgga
      781 cccagcggtt ccggcccgat cttgccgggc cgcgatgaag cacccatgac gatcaattca
      841 tgctcttcgg cttggctcaa gacttcatcg accaaatcgc cgacgatggt gaggtgccgc
      901 ttgatgcgat cgatgttgcg cagcagcggc agatatgatg catacggttg atcgatatgg
      961 ccgcgcgaac cggcgtacat cacggtgatc tctgcgttgg ccatctcggc ggcggccaat
     1021 gaaaaattca ccgcgagttt ggtgtatggt ccgccgcgta tcggcagcaa gatgcgatcg
     1081 atcttgttcc agtcggtggg ccgcaccagc gccagctcgc acggcgtttc agacaacact
     1141 tcggcgagtg gcacgcccat aatcgaagcg gccggatcac cgttccaatc caataggatc
     1201 agatccggtt cttccgcctg gatcgcttgc agtacttctt gccacggctc atagctgact
     1261 tgcaccgccg ctttcacgcg cagcggcaga tcggtatatc gattgcggat cgagttcatc
     1321 gctgcgcgca agccttgagc attttgcgcg ccgacgctca ggttttgatt tttcggcacg
     1381 cagacggccc cgatcaaaac gatctcaccc ttcatgcctt tggacgctgc gaccatttga
     1441 tgcagccacg ccattgcgag cggcagcaac acttccgctc gatcgacttg agtgattggc
     1501 agcatgattt gataagtttg cttcatcact cagctcgctt ctttaacaat ggctcaatgc
     1561 gatcggcgaa aattctttgc caaaagtatt cgctccgcac gcgcttctgc aatcgatgtg
     1621 ctccattcgt aattagctca cgcccgatca gcttggctaa ctcgatcgga tctgcgtccg
     1681 gcgcaaagta atgtgcatca gcttggccgg tttcgcgcag cgctggaatg tcggcgcaaa
     1741 agatcggcag gcgtgccaga cccgcttcca agatcggaat gccgaaacct tcttgttggc
     1801 tggggaagaa cactgcatct gccaaagtat acaagtcggc cagggtctca tccgggatca
     1861 acaattgatc agactggccg cactcgtaca gaaaatgcgc ggcgcgatcg atcttcagtt
     1921 cgcgccgcaa gttcaatagg ctttgcaagt aagcatcgtt cattggattg tgcgggccag
     1981 gtggaccact gacgatcaat cgcgcgtcga gttgagtttg tttccgcagt tccgctaaga
     2041 cccgaatccc cagctcgata tttttgcgct gcgtgatgcg tgcgggcagg agcaaaatga
     2101 gatcagccgc taacagatcg agtttagcca ctaactcacg tgtcagtgaa gtccagcgaa
     2161 aaaagttagc gggatcaacg ccgggcggaa tcacctcgat cgagtttgga tcgatctggt
     2221 agagttttgc taattcaact tgccgcgact ttgaaacggt tacgttctgc acattcggcc
     2281 acgcttcacg cagcaagttc catggataac cgtcatgcag ttgcgatcga tagagttcgt
     2341 tcgtccacgc cagatcatga tgccaggcaa tgacacgcaa ggggcgaccc gtctccgctt
     2401 cactacgagg gcgcttcgcg gaggcagggg gcgaggtgtc aatgaaattg cgcagcgcgg
     2461 tagtgagtgg cagattcttg tgcaaagtaa aaacattatg agcgatcagc acatcgacat
     2521 cggttagcaa cgatctcaat gtctcgagga tccgattgcg cagttgttca aaatccgatg
     2581 tgacttgtcc ggcatcgagc tgctgcttaa ctttcaacac atcggcattg cgcgaaccga
     2641 tctccggcac gacctgcacc ggaatccgat cgtcgaattg cgcgccgctg ccggtgatga
     2701 tcgaaacttc atagcccgcg tcgatcatca actgcgcatg acgcagcat
//
LOCUS       DASRTJ010000722         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_299836,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000722 DASRTJ010000000
VERSION     DASRTJ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299836"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..327)
                     /locus_tag="VFF70_14595"
     CDS             complement(223..327)
                     /locus_tag="VFF70_14595"
                     /inference="COORDINATES: protein motif:HMM:NF019144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45964.1"
                     /translation="MVCDDCRRELFDPSNRRYRYPFINYTNCGPRFTR"
     gene            complement(578..976)
                     /gene="hypA"
                     /locus_tag="VFF70_14600"
     CDS             complement(578..976)
                     /gene="hypA"
                     /locus_tag="VFF70_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZY45965.1"
                     /translation="MHELALTENILNTVLRYAADTNATRVTDVYFVVGQFSDVADESV
                     QFYWDLISPGTFCQSTQLHFEHRPTALKCLNCGGTFALPDDRVTCPACDSERVQIISG
                     DEFRLDSIQVDTPQAVIRSAIGELIEDLHD"
     gene            complement(1046..>3438)
                     /locus_tag="VFF70_14605"
     CDS             complement(1046..>3438)
                     /locus_tag="VFF70_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZY45966.1"
                     /translation="LEVPENTTILKAAEQAGIVIPTLCNHKDLTPYGGCRLCSVDIQG
                     LRLPATACTMLVAPGMIVHTESPALTRYRKTILELLLVGFYDAGYTHANGSSKPELDT
                     QFAHWVNYYGIDMKSAMRKKPFRRIDSDPNPFIWVDKNKCIMCTRCVRACAEVQGRFV
                     WTQAYRGYDLRIVAGAATTMLQARCESCGACVAHCPTGALDNKPSVKLGPPDRLVTTT
                     CSYCGVGCQLDLNIKDDVPGGRVLRVTSNAKAPINGDHLCVKGRYGYDFIQSPNRLKR
                     PRVRKYLLDGTARPNNRGLWTEVAWETALDIAAKGLQAARDQYGPNSIGLLTSGRSLN
                     EENYLMSKLARQLLGTNNIDCCDHVNHANVVDGLDAALGLNAQSNSLDDVAHEAKSIL
                     IIGSNTSEQQPIFGVKLRQSVLRHGCKLVVAHPDFINMAEYATLRLIHRPGTEAALVN
                     GLMHIILKNGWEDRAFIEKYTAGFQEFASSLNEFTPEYVVKLTGVEADTLQRAAEILA
                     KNRPTAIVWGVDLAKHPAGRHNVIGLINLQLLLGNFGVPGGGLIPLRTQNNSQGACDM
                     GGHPKFYPGYQPVDSESTRKKFEAAWGKGLPTKPGLTAAQMMGAAAEGKLRALFILSE
                     DVVAGAPASLNVRRSLHNCDFVVQLAAFDSETSYYADVLLPGVTFAEKTGTFTNTERR
                     IQMVHQAIDPQGGARPDWQIIMELARRIQRTSSNGEHSVWAYADTSQIMVEVAALTPI
                     YAGVSHDRLERANGLQWPVKNFSHPGTPILSMEHFAGGRGKFILIEDNIAESVTPI"
BASE COUNT          757 a          980 c          905 g          796 t
ORIGIN      
        1 cgatcggacg tttcagtcag aggcgacggt gggaattgaa ctcacagtga gggttttgca
       61 ggtcttcatg ggcgctttcg gagaacctca ttcgccttag cggactttat gactaagaat
      121 caatgccgaa ttattggttc ataacctcga gactcttaac cgtccatcag ggcaagccaa
      181 tagacattct gtagttattt ttcttacagc ataccgttgc attcacctgg tgaaacgcgg
      241 gccgcaattg gtgtagttga taaacggata gcgatagcgc cgattcgatg gatcgaataa
      301 ctcgcggcga caatcatcac acaccacaat gtcgggtgag atcgactgaa aggcaccggc
      361 ctgtgagact gaatgaacga tcgagaaagc cgaaaagcca tccgcttgcg cggcctgctt
      421 tcgatcgaat cgatcttggc gagcggcggt gcttcgatcg tcaagacttg aatgaagcga
      481 tcgatctgat attccacacc ctcgatcaaa atatcgacac ccgatgaggt gttgcgccct
      541 tcgccccgca cagtggccat ttcggattag gccatattta atcgtggagg tcttcgatta
      601 attcaccgat ggcagagcgt ataaccgcct ggggcgtgtc gacttgaata ctatcgagac
      661 gaaattcatc gccgcttata atttggacgc gctcgctatc gcaagccggg caggtgacgc
      721 gatcgtcggg tagcgcaaaa gtaccgccac agttcaagca cttcagcgcg gtgggcctat
      781 gctcgaaatg cagctgcgtg ctctggcaga atgtgcccgg gctgatgaga tcccaataga
      841 actgcacgga ctcgtcggcg acatctgaaa actgtccgac gacaaagtag acatccgtca
      901 ctcgagtggc gttcgtgtcg gctgcatagc gcaacaccgt attcaaaata ttttcagtca
      961 gcgccagttc atgcatctaa tttaagcact tcattcaccc agcgatcggg ctgcttaaat
     1021 actcccttgt tttctacagc ctgcattaga taggcgtaac actctcggcg atattatcct
     1081 caatcaagat gaacttaccc cgaccacccg caaagtgctc cattgacaag atgggcgtgc
     1141 cggggtggct gaagtttttc accggccact gcagaccgtt ggcgcgctcc aaccgatcgt
     1201 gtgacacgcc ggcatagatc ggcgtgagag cagcgacttc taccatgatt tgcgatgtgt
     1261 cggcatatgc ccagacgctg tgttcgccgt tggagctagt ccgctgaatg cgccgggcca
     1321 attccataat gatctgccag tccgggcgtg cgccgccttg cggatcgatc gcctgatgca
     1381 ccatttggat gcgccgctcg gtattcgtaa acgtgccggt cttctcggcg aaagtcacac
     1441 cgggcaacag cacatccgca tagtatgaag tctctgaatc gaacgcggcc agctgcacga
     1501 cgaagtcaca gttgtggaga ctgcgacgca cgttcaaact ggccggtgcc ccggccacca
     1561 catcctcact tagaatgaac agtgcccgca gtttgccttc ggctgccgcc cccatcattt
     1621 gcgctgcggt taggccgggt ttggtcggca agcctttgcc ccaggccgct tcaaatttct
     1681 tgcgcgtcga ttcgctgtcc accggctgat agcccggata gaatttcgga tggccgccca
     1741 tgtcacacgc gccctgcgag ttattctgcg tgcgcagtgg aatcagtccg ccgcccggca
     1801 cgccaaaatt tcccagcagc aattgcaaat tgatcaggcc gatgacgttg tggcggccgg
     1861 ccggatgttt cgccaagtcc acgccccaca caatcgcggt cggtcgattc ttcgccagaa
     1921 tttccgccgc gcgctgcaat gtgtctgctt cgacgccggt tagcttcacg acgtactctg
     1981 gggtaaactc attcaggctg cttgcgaact cttggaaccc cgcggtatat ttttcgataa
     2041 aggcgcgatc ttcccagccg tttttcagga tgatgtgcat caagccgttc actaaagccg
     2101 cctccgtgcc cggtcgatgg attagccgca gcgtagcata ttctgccatg ttgataaaat
     2161 ctggatgcgc gaccaccaac ttgcaaccat gccgcaaaac cgattggcgc agcttgactc
     2221 caaagatcgg ttgctgctcg ctggtattgg aaccgatgat caaaatagat ttggcctcat
     2281 gcgccacatc gtccagcgaa ttcgattgcg cgttcaagcc gagcgccgcg tccagcccat
     2341 cgaccacgtt ggcgtgattg acgtgatcgc agcaatcaat gttgttcgtg cccagcagtt
     2401 gccgagcaag tttgctcatc aaataatttt cttcgttcaa gcttcggccc gaagtcaaca
     2461 ggccaatgct attaggcccg tattgatcgc gcgccgcctg caatcctttc gcagcaatgt
     2521 ccaatgccgt ttcccacgct acttcggtcc acagcccgcg attgttgggg cgcgccgtac
     2581 cgtccaataa atatttacgg acacgcggtc gcttcaatcg attcggtgat tggatgaagt
     2641 cgtagccgta acggccttta acgcacagat gatcgccatt gatcggtgct ttcgcattcg
     2701 aagtgacgcg cagcacgcgg ccacccggca catcgtcctt gatgttcaga tcgagttggc
     2761 agccgacgcc gcagtatgaa cacgtcgtcg tcaccaatcg atcgggcggg ccgagtttga
     2821 cggatggttt gttgtccagt gcgccagtcg ggcagtgcgc cacgcacgcg ccgcacgact
     2881 cgcaacgcgc ttgcagcatg gtcgtggctg ctccagcgac gatgcgcaga tcgtaacctc
     2941 gatacgcttg cgtccacaca aaacggcctt gcacttcggc acacgcccgc acacagcgcg
     3001 tgcacatgat gcatttattt ttatcgaccc agataaaggg attgggatca ctatcaattc
     3061 ggcggaacgg tttcttcctc atcgcacttt tcatgtcaat gccgtagtag ttcacccagt
     3121 gggcgaactg ggtatcgagt tctggtttgc tcgatccatt cgcgtgggta taaccggcat
     3181 cgtaaaatcc aactaacaat aattccaaaa ttgttttgcg ataacgcgtc aacgcgggac
     3241 tttccgtgtg cacgatcatg cccggggcaa cgagcatcgt gcaagccgtg gcgggcaagc
     3301 gcaagccttg aatatccacc gaacacaaac ggcagccgcc atacggcgtc aagtctttgt
     3361 ggttacacaa cgtcgggatg acgatcccgg cctgctcggc ggctttcaag atcgttgtgt
     3421 tttcaggaac ttccagag
//
LOCUS       DASRTJ010000723        12791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_374853,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000723 DASRTJ010000000
VERSION     DASRTJ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374853"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VFF70_14610"
     CDS             complement(<1..1449)
                     /locus_tag="VFF70_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45967.1"
                     /translation="MIRPADNPRWPGRRTEAAALFLILVVAAFFRLNQIDRIPPGFTH
                     DEADHGHDAVAILNGARPIYETVGYGREPLYDYIVAMLMPLLGGPHYLALRLTSVLAG
                     LLLIVIAHFWIRRSFDVPIALLTSAGLAVSFWAISTDRQALRSALLPTLFTVAVLFAW
                     QAVKPYSLNKLTGLSQRTLRIGSYLIAGLFICLTLYTYLAARVLPGMFVLLWIYLLLF
                     HRSVWKENWWGIIGMIAIGAALALPMFTYLSAHPGAEYRFTQLSGPIDRLFAGNPNEL
                     LANIFSALGMFTVSGDTLSLYNNPGQPILNWALGGMFYIGIVISFIRFRKIEYAFAIF
                     WLMVAIFPSLLTGVVASNLRSIAAQPVVYLFVAIGLVEAVRFFDRVIESHTLRSIPLI
                     ALLAATTIYAYHDYFDVWGQARDVRVAYHTTLVEVAHYLDRSTSDQLVVGISSIYPNR
                     FHDPASMQMLLQRRDLSLHWFTAAFVDMDSVPH"
     gene            complement(1598..3235)
                     /gene="groL"
                     /locus_tag="VFF70_14615"
     CDS             complement(1598..3235)
                     /gene="groL"
                     /locus_tag="VFF70_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958812.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZY45968.1"
                     /translation="MAKQIVFTTEAQQALKRGVDILASAVATTLGPKGRNVALDKKFG
                     APTVTHDGVTVAKEIELQDPYENMGAQLLKEAATKTNDIAGDGTTTATVLAQSIVNEG
                     LKNVAAGANPMQIKRGLEMATDAVVAAIKKMSTPVADKHEIANVASISAADTEIGDLI
                     AEVMDKVGKDGVVTVEESKGREFETEYVEGMQFDRGYISQYFVTNTETMEAVIENAYI
                     LIHDKKISAATDIVPLMEKLLQKGKRDLVVIAEDVDGEALATLVLNKLRGTFNLLAVK
                     APGFGDRRKAMLQDIATLTNGTLITDELGRKLESVTLEDLGSAAKIVADKDNTTIIEG
                     KGNTKDIKGRIEQIKAEIENTTSDYDKEKLQERLAKLSGGVAIIRVGAATETELKEKK
                     HRVEDALSATRAAVEEGIVPGGGVALINAIDALKKVKSDEPDIQTGVTIMRRALEEPM
                     RRIAANAGQDGAVIIDTVRRVSKEKENKNIGFNVLSGEFEDMVKSGIIDPAKVTRSAV
                     ENAASIAAMILTTDALITDVPEKNPAPMMPPGGGGDF"
     gene            complement(3255..3560)
                     /gene="groES"
                     /locus_tag="VFF70_14620"
     CDS             complement(3255..3560)
                     /gene="groES"
                     /locus_tag="VFF70_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZY45969.1"
                     /translation="MAAKKMGLKPLGDRVVIEPIEKEEKTASGIILPETAKEKPQEGT
                     VLAAGPGRTDDDGKRIAMDVKVGDTVLYAKYAGTEIKIDDRKLLILKESDILAIVEK"
     gene            complement(3709..4038)
                     /locus_tag="VFF70_14625"
     CDS             complement(3709..4038)
                     /locus_tag="VFF70_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HZY45970.1"
                     /translation="MKLMLAVIQNEDEDSLVQALEGKNIGATRIGSSGGFLRASNVTL
                     MIAVDDTQVEAVFDLLRIHCKRRTRHLHPLLPNLEARERFLGSIPVEVGGAAVFVLSV
                     DRMEKLG"
     gene            complement(4130..5392)
                     /locus_tag="VFF70_14630"
     CDS             complement(4130..5392)
                     /locus_tag="VFF70_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY45971.1"
                     /translation="MYLDRSRSRHTSPRRIAILIILIGAGLFVIANQNALRQQLIPPP
                     TPTATRTARSYVVEAQSLAEAGQIDDAIGSYIQATALDSANIDVLIDLSRLLALTQRS
                     DEAVQWALRAAQLSPQSAKAKAALAMALDWNAASLEGRGRVVDAQPEYQQAIDAAHAA
                     ISIDPNYPEAYAYLAETYADLNRWADAIAAAQKAVDLNPNRVDVQRALGYVRESQGNY
                     SGAAQAYEQALKFAPNLPYLYIVLGRNYRVIASVSDASKYQKAIDAFKRAVAIDSNNV
                     VAYDELGWSYYNFDQLTEAQKALEQAVAIDPQSWSAAEHLAVTYYARRNYEDASVTFK
                     TAIGLMNQTFDGDHYCVTSTSRACDRLIEAYYTVGLAFYYLSKCETESYPAFQKALVL
                     RPDEPNAKFGIQLCNDALVTPTPTPKPK"
     gene            5506..7311
                     /locus_tag="VFF70_14635"
     CDS             5506..7311
                     /locus_tag="VFF70_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45972.1"
                     /translation="MNCRNCGARVEPDDLVCGNCGHVIDSAPQLAPKNSMTLPKPIKP
                     RRPLSPTGILIIIFSCGMFTLIAASILGGVATGLQDRETDRQAKADQYYHAGVTHFAT
                     GQLQLAEVDFLYVLKLDPNYPGASGQLSQVQTRLTVVPTPTAAVSQTKVIDQLFQTGQ
                     SAYQTKNWARAIDVLSQVRAIDPKYQTDKVNSLLYQAALTYGLELLKTDRLEEGVAYL
                     DQAAYIQPLPNDSALNSQYAKMYLTARGYWNVDWQKAIDRFSELYAIGPGYKDTFTRL
                     VEAHINYGDQLSQSLDFCTARQNYEAANQLRPDPNLQPKIVDTQQKCLTATPSISGTL
                     TAGNVDGTPGASQTLSGLFTGRLAFPMSDASGSRIFAASAGSPSIYIAAVGDQPELQR
                     NGSNMVYRLTGVGINLVNLAIGSIETIAPPGASSPTFSPDGTRVIYSLHGRLYVVGAH
                     GGAPVDLGAGTTPVWGPTDLLAFSGCDGSGCGIMLRNPDQSNPPTRLTGSPQDIPTSW
                     SPDGANISYFSNVTGAYNLFFVNTFGGVQQVTIGSSNSIAGAWGPDGAHIAFLSDRDG
                     AWSVYIARFDGTEAQKIINAPQSSDWINARLSWMP"
     gene            complement(7349..8560)
                     /locus_tag="VFF70_14640"
     CDS             complement(7349..8560)
                     /locus_tag="VFF70_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZY45973.1"
                     /translation="MAEQHILVADDSEPIREVIATFLEMRGYRIDQAADGQRALDLYR
                     VRQHDLVVTDLQMPRLEGLPLLKLIKTINPDAQVIILTGNATLQTAVEALRLGAYDYL
                     LKPIEDMDSFDRLVDRALRHGRLLQENKRLVEELRQGNTRLEAQVAERTRDLKAANES
                     LLSLDKLKNDFVSMVSHELRTPLSVILLEAELLNQAGVSLSPQKIKQTYSALMTNAGR
                     LRAQIENLLDFSLLERGELELHLEPCSVNQAIREVIDLYQARADEKKLRLIFDRQPTS
                     VLIVTADMPRLRSALIQLVDNAVKFTPENGTITLTAHGPTTIPGSEIRAVAMVVKDTG
                     IGVPLDRQPVLFKLFSQADMSSTRRYGGMGIGLALANRIVTAHHGQITFHSEPDNGSV
                     FAMWIPTRSDK"
     gene            8710..9432
                     /locus_tag="VFF70_14645"
     CDS             8710..9432
                     /locus_tag="VFF70_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY45974.1"
                     /translation="MTSSQHILIVDDEPNVGDVLGIYLRRENYQVSIARDGQTALALI
                     EQTPPDLIVLDLMLPKIDGLSIVRRVRGDEGHAGLFPEIPIVILTAKGEESDRISGLD
                     LGADDYVIKPFSPRELVSRVKAVLRRSKGASVAPERPLSFGPLSIDPKSREVSLNDRE
                     CSLTAKEFDLLYFMARHTRQVFTRDQLLEHVWDLSEYIDPSTVTVHIRRLREKVESDP
                     GKPEWIHTVWGIGYKFDPEEAE"
     gene            9530..10552
                     /locus_tag="VFF70_14650"
     CDS             9530..10552
                     /locus_tag="VFF70_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZY45975.1"
                     /translation="MNFLKRLAPPVAFALGISGVLITAVVTIEVVMRPPAKDLNVIIG
                     YMIISSLGSLGAGYVLYRFGWWRWSGHIFFTLTLGVVIQAIVVFFNVWLTARSMLINQ
                     HDLDLTSMLLLFGIGIGIAFGAFAAASLTQRITQLRHAARSIASGDLSTRVDVKGHDE
                     LAELGTTINSMTTQLEASAQKQKELDHMRRDLIAWASHDLRTPLTSIRVVVEALADGV
                     VDDPDSRQRYLASLQADVRSLSRMIDDLFEIAQIDAGGLKLDLIPCSLHDVISDVLTR
                     MRALADARQITLDGRVDPALQPVQIDPPKFERVLVNLIDNALRHTSAGGRVEVLADRA
                     GENNRI"
     gene            10553..10765
                     /locus_tag="VFF70_14655"
     CDS             10553..10765
                     /locus_tag="VFF70_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY45976.1"
                     /translation="MTDTGDGIDPRDLPFVFDRFYRGDASRSRTTGGAGLGLAIAKGI
                     VEAHRGQIRVRSELARGTTFEVLLPH"
     gene            11183..12520
                     /gene="gntE"
                     /locus_tag="VFF70_14660"
     CDS             11183..12520
                     /gene="gntE"
                     /locus_tag="VFF70_14660"
                     /inference="COORDINATES: protein motif:HMM:NF041362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HZY45977.1"
                     /translation="MALTRTNTQKLFDRAKKVMPYGVNSNFRYWGDDKTHVFVRGEKC
                     YVFDADENRYIDYRLGFGPVILGHGYPAVVERMTRALKDGGVYAATHPLEIIVAERIA
                     RMTGMDKVRLVNSGTEATMHALRIARAHTNREKFIKFEGAYHGMHDNVLFSTSMSLSR
                     TFGSRRSPVQVASGSGIPRALNEYVITLPFNDPEMLHKTLQAKWGDIAAILIEPIMGN
                     AASLMPEPGFLQTIRRLCDEYGIVMIMDEVKTGFRIANGGAQEYFGVKADLATYAKAM
                     ANGYPLAAIAGMDEVMSVIEPGKISLGGTYCGNIPGTAATDATLEILEKQDILGSIGL
                     RGERLKKGIDQILDEADLPHVVMGPPQMFGFFIGSHKVPRDYRDLVAGADIEFYEKFA
                     FEMIDRGVMPEPDNREPWFLCYEHTDAVVDETLNIFQDALTATLSKHSPHLIQ"
BASE COUNT         2929 a         3580 c         3381 g         2901 t
ORIGIN      
        1 gtgcggcaca ctatccatat cgacaaacgc tgcggtgaac caatgcagcg atagatcccg
       61 tcgctgcaac agcatctgca tcgaggccgg atcgtgaaag cgattcggat agatcgacga
      121 aatcccgacc accaattgat cgctggtaga tcgatcgaga taatgcgcaa cctcgaccaa
      181 tgtggtgtga taagctacgc gcacatcgcg cgcctgaccc cacacatcga agtaatcgtg
      241 ataagcatag atcgtcgtcg cggccaagag cgcgatcaat gggatcgatc gcagtgtgtg
      301 cgattcgatc acgcgatcga aaaaccggac agcctccacc aaaccgatcg cgacgaaaag
      361 atagaccaca ggttgcgccg cgatcgatcg caaattgctg gcaacgacac cggtcagcag
      421 actgggaaag atcgcaacca tcagccagaa gatcgcaaag gcgtattcga tcttgcgaaa
      481 gcgaataaac gaaatgacga tgccaatata aaacatcccg ccgagcgccc aattcagaat
      541 aggttggccg ggattgttgt aaagcgacag tgtgtcgccg ctcaccgtaa acatccccaa
      601 cgcgctaaaa atattggcga gcaattcatt gggattgccg gcaaataagc gatcaatcgg
      661 gccgctcaat tgcgtgaagc gatattcggc gccgggatgc gcggacaaat aggtaaacat
      721 cggcagcgcc aatgcagcgc caatcgcgat catgccgata atgccccacc aattttcttt
      781 ccagaccgat cgatggaaga gcaataaata aatccacaac aatacaaaca tgccgggcag
      841 gacgcgcgcc gctaaataag tgtagagcgt caaacaaatg aacaagcctg cgatgagata
      901 actaccgatg cgcaaagttc gctgcgatag gccagtcaat ttattgagtg agtacggttt
      961 cactgcttgc catgcaaata gaactgcgac agtgaacaaa gtgggcaaca atgcagagcg
     1021 caacgcctgc cgatcggtgg agatcgccca gaaggagact gccaagcctg ccgaggtcag
     1081 caaagcaatc gggacatcaa atgaccgccg gatccagaaa tgagcgatca cgatcaacaa
     1141 aagtccggcg agcacggagg tcaagcgcag cgccagataa tgcggcccgc ctaacagcgg
     1201 catcagcatg gcgacgatgt aatcgtataa cggttcgcgt ccgtaaccga cggtttcata
     1261 gatcgggcgt gcgccgttca ggatggcgac agcatcatga ccgtgatcag cttcatcgtg
     1321 ggtgaaaccc ggcggaatgc gatcgatctg gttcagacgg aagaaagccg ccacgaccaa
     1381 aattaaaaac aaggccgcgg cttcggtgcg tcggccgggc cagcggggat tatcggcagg
     1441 tcgaatcatc gtgagcgatg ataccctaaa acgtgaaaag agacgagtga gaagtgaaga
     1501 gtaacgagta attgcctctt gctcgttact cattagctaa caaaaaaccc gactcgcaat
     1561 gaagtcgggt ttctcaagac aaattctacc gggcgaacta gaagtcgcca ccgccaccgg
     1621 gcggcatcat cggggccggg ttcttctccg gcacatcggt gattaacgca tcggtggtca
     1681 gaatcatggc cgcgatactg gccgcattct ccaccgcgct gcgtgtgacc ttggccggat
     1741 cgatgatgcc cgacttgacc atgtcttcga actcgccgct caacacgttg aaaccgatat
     1801 tcttgttttc cttctctttc gatacacggc ggacggtgtc gatgatgacc gcgccgtctt
     1861 gaccggcgtt ggccgcgatg cggcgcatcg gctcttcaag cgcgcggcgc atgatcgtca
     1921 cgccggtttg aatatccggc tcgtccgact tgaccttctt cagcgcatcg atcgcattga
     1981 tcaacgccac acccccgccg ggcacaatgc cctcttccac agcggcgcgc gtcgcgctca
     2041 aggcgtcttc gacacgatgt ttcttttcct tcagttcggt ctcggtcgcc gcgcccacgc
     2101 ggatgatcgc cacgccgccg gacaacttcg cgagccgctc ttgaagcttt tctttgtcgt
     2161 aatcactggt cgtgttttcg atctcggctt tgatctgctc gatacggccc ttgatatctt
     2221 tggtgttgcc cttgccttcg atgatcgtgg tattgtcttt atcagccaca atcttagcgg
     2281 ctgaacccag atcttccaat gtgacggact ccagcttgcg gcccagttca tcggtgatga
     2341 gcgtgccgtt ggtcaaggtg gcaatgtctt gcagcatcgc cttgcgtcga tcgccaaaac
     2401 ccggcgcctt gactgccaag agattaaacg tgccgcgcag cttattcaac actaaggtgg
     2461 ccagcgcttc gccatcgaca tcttcggcga tgacgacgag gtcgcgcttg cccttctgca
     2521 gcaatttttc catcagcggc acaatatcgg tcgccgcgct gatcttctta tcgtggatca
     2581 agatgtacgc attttcgatt acggcttcca tcgtctccgt gttggtgacg aagtattggc
     2641 tgatgtagcc ccgatcgaac tgcatgcctt cgacgtactc agtctcaaac tcgcggcctt
     2701 tgctttcctc tactgtgacc acgccatcct taccgacctt gtccattact tcggcgatga
     2761 gatcgccgat ttctgtatcg gcggcggaga tcgacgctac gttggcgatt tcgtgcttgt
     2821 cagccacggg cgtgctcatc ttcttgatcg cggcgacaac ggcatccgtc gccatttcca
     2881 ggccgcgctt gatctgcatc ggattcgcgc cagccgctac gttcttcaag ccttcgttaa
     2941 cgatcgattg cgcgagaacc gtcgcggtcg tcgtgccatc gccggcaata tcgttggtct
     3001 tggtcgcggc ttccttcaac agctgcgcgc ccatattctc atatggatct tggagttcga
     3061 tctctttggc cacagtcaca ccatcgtggg taaccgtcgg ggcgccaaat ttcttatcga
     3121 gcgccacatt gcgtcccttg ggacctaagg tcgtggcaac tgcgctggcg agaatatcga
     3181 cgccgcgctt cagtgcttgt tgtgcttcag tagtaaatac gatttgttta gccatggctc
     3241 atgctcctgt aagattactt ctcaacaatg gccagaatgt cgctctcttt gagaatgagc
     3301 aacttcctgt catcgatctt gatttcggtg ccggcgtact tcgcatacaa cacggtatcg
     3361 ccgactttaa catccatcgc gatgcgcttg ccatcgtcgt ccgttcgacc gggacccgcg
     3421 gccaacaccg tgccttcctg cggcttctct ttcgcggtct cgggcagaat gatgccgctg
     3481 gcggtcttct cttccttctc gattggctcg atcacaactc gatcgcccag gggtttaagc
     3541 cccattttct ttgctgccat actagatgcc tcctgaatag gttatgagat ttaaaatgat
     3601 gcctgtgaca caaacaaatg tcggttagca ctcaccaagt gagagtgcta accgacctaa
     3661 atataccaca tcgctcacta aattgtcaag ggtgcgtcag gaattttttc agccgagctt
     3721 ctccatccga tcgacggaca aaacaaaaac ggccgcgccc cctacctcca cgggaatcga
     3781 accgagaaat cgctcacgcg cttccaggtt gggcagcaat ggatgcaaat gccgtgtgcg
     3841 gcgtttacag tgtatccgca gtagatcgaa caccgcttca acttgggtat catccaccgc
     3901 aatcatcaac gtcacattgc tggcgcgcag aaacccgccg ctcgatccga tccgcgtcgc
     3961 gccgatgttc ttgccttcca gcgcctgcac gagtgaatct tcatcttcgt tttgaataac
     4021 cgctagcatc agtttcatgt cgcctcccta tgatacgtga cgagtaatga gtactttttg
     4081 cgatacacga tatgcgatac gccattcgct atctgccatc cgccagccgc tacttcggtt
     4141 taggcgtagg tgtgggcgta accagcgcat cgttgcacaa ttgaataccg aacttggcat
     4201 tcggttcatc ggggcgcagg accaatgcct tctgaaacgc cggataggat tcggtttcgc
     4261 atttcgacaa gtaatagaag gctagaccca cggtgtaata ggcttcgatc aaccgatcac
     4321 aggcgcggct ggtcgaggtg acgcaataat gatcgccatc gaaagtctga ttcatcaacc
     4381 cgatcgccgt cttgaaggtt actgaagcgt cttcgtagtt gcggcgcgca taataagtga
     4441 cggccaaatg ctcagccgcc gaccatgatt gcggatcgat cgcaacggcc tgctccaagg
     4501 ctttttgggc ctccgttagc tgatcgaagt tatagtagct ccagcccaat tcatcgtaag
     4561 cgaccacatt gttagaatcg atagcaacgg cacgcttgaa cgcatcgatc gctttttgat
     4621 atttggatgc atcactcaca ctcgcaatca cgcggtagtt gcggcccagc acgatataca
     4681 agtatggcag gttgggcgcg aatttgagcg cttgctcgta agcctgggcc gcgcccgaat
     4741 agttgccctg gctttcccgg acatagccca aagcgcgctg cacatcgacg cgattaggat
     4801 tgagatcaac ggccttctgt gccgcagcga tcgcatcagc ccaacgattg agatcggcgt
     4861 aggtttccgc cagataagca tacgcttcag gataattggg atcgatcgag atggcagcat
     4921 gcgcagcatc gatcgcctgt tgatattccg gttgtgcatc gactacgcgg ccgcgacctt
     4981 ccaagctggc tgcattccaa tcgagcgcca tcgctaaggc ggccttcgct ttggcagatt
     5041 gcggcgagag ttgagctgcg cgtagagccc attgcactgc ctcgtcgctg cgttgggtca
     5101 gcgccaacaa gcgcgacaga tcgatcaaca catcgatatt ggctgaatcc aacgcggtgg
     5161 cttgaatgta agaaccgatg gcgtcatcaa tctgaccggc ttcagccaac gattgcgcct
     5221 cgacgacgta cgatcgagca gtgcgcgtcg ccgtcggtgt gggcggcgga atcaattgtt
     5281 gacgcagcgc gttttgattt gcgataacaa acaaaccggc accgatcaag ataattaaaa
     5341 tggcaatgcg gcgcggcgat gtatggcgcg aacgggatcg atctaggtac atggcaacat
     5401 cttagcacgg cgattcgcgt gagtcaatcg gagatacaag gttcgaagtt agaatatgct
     5461 ataatctcgc gcgatatatt ggtttcatca tttgctggag catacatgaa ctgtcgcaat
     5521 tgcggcgcgc gagtggaacc tgacgatctg gtctgtggta attgtggcca cgtcatcgac
     5581 tctgcaccgc agctggcccc gaaaaattca atgaccctgc ctaaaccgat caagccgcgc
     5641 cgccccttgt cgccgacggg gatcttgatc ataatctttt cctgcggcat gttcacgctg
     5701 attgcagctt cgatcttggg tggagttgca accggcctgc aggatcgcga gaccgatcgg
     5761 caagccaagg ccgatcagta ttatcacgca ggcgtcaccc atttcgcaac cggccaatta
     5821 caattagccg aagtggactt cctgtacgtt ttgaaactgg atcccaacta tccgggcgcc
     5881 agcgggcagc tgtcgcaagt ccaaactcga ttaacggtag tccccactcc aacggcggca
     5941 gtctcacaga ccaaagttat cgaccaactt tttcagaccg gacaaagcgc gtatcaaacc
     6001 aaaaactggg ccagggcaat cgatgtgctg tcacaggtcc gcgcgatcga tccgaaatac
     6061 cagaccgata aagtcaacag cctgttgtat caagcggcac tgacttacgg gctggagttg
     6121 ttgaagaccg atcggcttga agaaggtgtg gcttacctcg atcaagcggc ttatattcaa
     6181 cccctgccca acgactcggc gctgaactcg caatatgcca agatgtattt gacagcgcgt
     6241 ggatattgga acgtcgattg gcagaaagcc atcgatcgat tcagcgagtt gtatgcaatc
     6301 ggccctggct ataaagatac cttcacgcga ttagttgaag cgcacatcaa ttacggcgat
     6361 cagttgtcgc aatcactaga cttctgcacg gcgcggcaaa actacgaagc agccaaccag
     6421 ttgcggcccg atccgaactt gcagccgaag atcgtcgata cgcagcaaaa atgcttgacg
     6481 gccacaccat cgatcagcgg tacgctgacg gcaggcaacg tcgatggcac accaggggcg
     6541 tcgcaaaccc tatcgggatt gttcacgggg cgcctcgcat tcccgatgtc tgatgcaagc
     6601 ggttcgcgca ttttcgccgc cagcgcgggc agtccatcga tctatatcgc ggcggtgggc
     6661 gatcaacctg aattgcagcg caacggatcg aacatggtct atcgcttgac tggcgtgggg
     6721 atcaacttag tcaatctagc aatagggagt atcgaaacaa tcgcaccacc tggcgcatcg
     6781 tcaccaactt tctcgccgga cggcacccgt gttatttatt cattgcacgg ccgcttgtat
     6841 gtcgttggtg cacacggcgg cgcaccggtc gatctgggtg ctggcacgac accggtatgg
     6901 ggaccaactg atctgttggc gttttctggt tgcgatggca gtggctgcgg catcatgctg
     6961 cgcaatccgg atcaatcaaa tccgccaacg cggttgaccg gcagcccgca ggatattcca
     7021 acttcatggt cgcctgacgg cgcaaacatt tcctatttct caaatgtgac gggcgcttat
     7081 aatttattct tcgtcaatac attcggcggt gtgcaacaag tgacgatcgg gagcagtaac
     7141 agcattgcag gtgcgtgggg gccagacggc gcacatatcg cgttcttatc ggatcgagat
     7201 ggagcgtgga gcgtttacat tgcccgtttc gatggcacag aggcgcagaa gataataaac
     7261 gcgccgcaaa gttctgattg gatcaacgcg cgtctgtcgt ggatgccata aagaaatgct
     7321 tcaggctcaa ctgttcatcg atcgctgctt atttgtccga gcgagttgga atccacatgg
     7381 caaagacact gccattatct ggttcgctat gaaatgtgat ctgaccgtga tgagccgtga
     7441 caatccgatt ggccaacgcc aagccgatgc ccatgccccc gtaacggcgt gttgaactca
     7501 tatcggcttg actgaataac ttgaacagca ccggctgccg atcgagtggc acgccaatcc
     7561 ccgtgtcctt gacgaccatc gccacggctc gaatttccga gccgggtatc gtcgtgggtc
     7621 cgtgcgcggt aagcgtgatg gttccattct ctggcgtaaa ttttactgcg ttatcgacga
     7681 gttgaatcaa ggcactccgc aagcgcggca tatcggccgt gacgatgagc accgacgtcg
     7741 gctgccgatc aaatatcaac cggagttttt tttcgtcggc gcgggcttga tacaaatcga
     7801 tcacttcgcg gatagcttga tttaccgaac acggttccag gtgcaattcc aactcgccgc
     7861 gctccagcaa gctaaagtcc agcaaattct caatctgcgc tcgcagacgt ccggcatttg
     7921 tcatcaacgc cgaataagtt tgcttgatct tttgcggtga gagcgagacc ccggcctggt
     7981 tcagcagctc agcctctaac aaaatcacgg acagcggcgt gcggagttcg tgcgaaacca
     8041 ttgagacaaa atcattcttc agtttgtcca gactgagcaa tgattcattc gccgccttca
     8101 aatcgcgcgt ccgctcggca acttgcgcct ccaggcgagt attcccctga cgcaattctt
     8161 ccaccaagcg tttgttctcc tgcaagagac ggccatgcct caacgcccga tcgaccaacc
     8221 gatcaaaaga atccatatct tcaatcggct tcaacaaata atcgtaagcg cccagccgca
     8281 aggcttccac cgccgtctgc agggtggcgt tgccggtcaa gataatcact tgcgcgtctg
     8341 gattgatagt cttaatgagc ttcaacagcg gcaaaccctc cagcctgggc atttgcaaat
     8401 cggttacgac cagatcgtgc tgccgcaccc gatacagatc caacgccctt tgcccgtctg
     8461 cggcctgatc gatccggtaa cctcgcattt caagaaaggt cgcgatcact tcgcgaatcg
     8521 gttcgctgtc gtcggcaact aaaatatgct gttcggccat aaatctcctt gcacttgcat
     8581 tgtaacatga tctaacacaa ccgatcatcg tttcagctaa cttgcctacc gccggaaaga
     8641 ctgctacgat aaacgcgtca gcgtaatcta ggcgtaatcc ggtatggcta tactgggagc
     8701 atgctcacga tgaccagcag tcaacacatc ttaattgtcg atgatgaacc gaatgtcggc
     8761 gatgtgctgg gcatttatct ccgccgcgaa aactatcagg tctctatcgc gcgcgatggt
     8821 caaaccgcgt tagcgctgat cgaacagacc ccgcctgatc taatcgtgct cgatttgatg
     8881 ctgcccaaga tcgatggctt gtcgatcgtc cggcgtgtgc gcggcgacga aggccatgcc
     8941 ggtttattcc cggaaatccc gatcgtgatt ctgaccgcca aaggtgaaga gtccgatcga
     9001 atcagcggcc tcgatctggg cgcagacgac tatgtgatta aaccgttcag cccgcgcgag
     9061 cttgtcagcc gcgtcaaagc cgtgctgcgc cgcagcaaag gcgcatcggt cgcgccggaa
     9121 cggccgctgt ccttcggccc actctcgatc gatcccaaat cccgtgaggt cagcctgaac
     9181 gatcgagaat gcagtttgac cgcgaaagaa ttcgatttgt tgtattttat ggcgcggcac
     9241 acgcggcaag ttttcacgcg cgatcaattg cttgagcacg tgtgggactt gagcgaatat
     9301 atcgatccta gcacggtcac ggttcacatt cgaaggctgc gtgaaaaagt tgagtctgat
     9361 cctggcaagc cagaatggat tcataccgtc tggggcatag gttacaaatt cgatcctgaa
     9421 gaggctgaat gaccgcacgc ctagctgcaa gacattttga aatccgatcg cacgcgcgct
     9481 gctattcttt cttagcggca taacaccttc gcctgagtca agaatattca tgaactttct
     9541 taaacgtctc gctccaccgg ttgcttttgc gcttggcatc agcggcgtgt tgatcaccgc
     9601 cgtcgtcaca atcgaagtag tcatgcgccc gccggccaaa gacttgaatg tcattatcgg
     9661 ttacatgatc atctccagtc tcggttcgct cggcgcgggt tatgtgctgt atcgctttgg
     9721 ttggtggcgc tggtcgggac atattttctt tactcttacc ttgggcgtgg tgattcaagc
     9781 cattgttgtg ttctttaatg tatggctgac ggcgcgcagc atgttgatca atcagcatga
     9841 tcttgatctg accagcatgc tgttgttgtt cggtatcggt atcggcatcg ccttcggcgc
     9901 attcgccgcc gcttcgctga cgcaacgcat cactcaactg cgccacgccg cgcgatcgat
     9961 cgccagcggc gatttatcga cccgcgttga cgtgaaaggt catgatgagt tagccgaact
    10021 cgggacgacc atcaattcga tgaccacgca gttggaagcg agcgctcaga aacaaaagga
    10081 actcgaccac atgcgccgcg atctgatcgc gtgggccagc cacgatctgc gaacgccgct
    10141 gacttccatc cgcgtggtgg tggaagcgct ggccgatggt gtcgtcgatg atcctgattc
    10201 acggcaacga tacttagcca gtttgcaagc cgatgtgcgc agcctcagcc gcatgatcga
    10261 tgatctcttc gagatagcgc agatcgatgc gggcggctta aaactagacc tcatcccctg
    10321 ctcgctgcat gatgtcatct cggacgtatt gactcgcatg cgagcgttgg ccgacgcccg
    10381 acagatcaca ctcgacgggc gggtcgatcc ggcgctgcaa cctgtccaaa tcgatccacc
    10441 gaaattcgaa cgagtgctgg tgaacttgat cgacaacgcg ctgcgtcaca cttcggcagg
    10501 cggccgcgtc gaggtgttag ccgatcgagc tggcgagaac aatcggatat agatcaccga
    10561 cacaggggac ggcatcgatc cacgtgattt gcccttcgtg ttcgatcggt tctatcgcgg
    10621 cgatgcttct cgatcgcgca caacaggcgg cgcgggcctg gggctggcga tcgccaaagg
    10681 catcgtcgaa gcgcatcgcg ggcagatccg cgttcgcagt gagttggcgc gcggtacaac
    10741 cttcgaagtg ttgctcccac actaaggatg tgatgctttt cccaattttg acaaccattg
    10801 gcttgacagg aactgcaccc caagcctaca attgaggcat gtcagcccct aagcattgga
    10861 ttcctactga taccgccgaa gacggccgcc tgtttatccc ctggcggata cgcaacccgc
    10921 tactcgccga tcatcccctg atctttctgg atcattgttg ggtgatgtgg ttcaccgccg
    10981 atcaaagacg gtgcctaatt ttttatacgc ttgatcgttg acttcgatcg atccggccgc
    11041 aacgtcgcca gcctgtttga tcgacaacga tccagcatcc cgcttaaccc gatcgactca
    11101 gctcaatcga tcgggatttg ttgcaattcg caataattaa aaaatcggac accactatta
    11161 gtgattctgg agaacagtta tcatggcact cactcgaacg aacactcaga aacttttcga
    11221 ccgagccaag aaagttatgc cgtatggtgt caactccaac ttccgctact ggggcgacga
    11281 caaaacccac gtcttcgtcc gcggcgagaa gtgctacgtc tttgacgccg acgagaaccg
    11341 ctacatcgat tatcgactgg gctttggccc ggtgattctg ggtcacggtt atccggcggt
    11401 ggtcgagcgc atgacccgcg cgcttaaaga tggtggcgtg tatgccgcga ctcatcccct
    11461 tgaaatcatc gtggctgaac gcatcgcccg gatgacgggc atggacaagg ttcggttggt
    11521 taattccggc accgaggcaa ccatgcatgc gctgcgtatc gcgcgcgcgc acacgaatcg
    11581 cgagaagttc atcaagtttg aaggcgcgta tcacggcatg cacgacaatg tcttgttctc
    11641 aacctcgatg tcgttgagcc gcacgtttgg ctcgcgccgc agtccggtgc aagtggcttc
    11701 gggcagcggc attccgcgcg cgctgaatga gtacgtcatc accctgccct tcaatgaccc
    11761 ggagatgctg cacaagacgc tgcaagccaa atggggcgat attgccgcga tcctcatcga
    11821 accaatcatg ggcaacgcgg ccagtttgat gccggagccg ggtttcctgc aaactattcg
    11881 ccgcttgtgc gatgaatatg gcatcgtcat gatcatggat gaagtcaaga ccggtttccg
    11941 cattgccaac ggcggcgcgc aggaatattt cggggtcaag gctgatctgg ctacgtatgc
    12001 caaagccatg gccaacggtt atccgctagc ggccatcgcg ggcatggacg aagtgatgag
    12061 cgtcatcgag ccgggcaaga tatcgctggg cggcacgtac tgcggtaaca ttccgggcac
    12121 cgcggcgacc gatgccacgc tggagatttt agagaaacag gatattttgg gcagcatcgg
    12181 cctgcgcggc gaacgactaa aaaagggtat tgatcaaatt ctcgatgagg ccgacctgcc
    12241 gcacgtggtc atggggccgc cgcagatgtt cggtttcttc atcggcagcc ataaagttcc
    12301 gcgcgactac cgcgacctgg ttgcgggcgc tgatatcgaa ttctatgaga agtttgcctt
    12361 cgagatgatc gatcgcggtg tcatgccgga gccggacaat cgcgagccgt ggttcctatg
    12421 ttacgagcat accgatgcgg tcgtcgatga gacgctgaac atcttccaag acgcgctcac
    12481 agccacgctc agtaagcatt cacctcacct gattcagtaa tcgatagaaa cgagaagccc
    12541 ctcgaaatag cgaggggctt ttttatttga agtgtccgcc acagtaacgt ataagagcgg
    12601 cgtgcgcaga tcgacacttc gcaacatctt gattagctta gcgacattca acttcgccac
    12661 attgatcgtt tggcatgcga tacgtcgatc acagattgct gaagctcgcc aaccatatcg
    12721 aactcagatt aataagttgg gcaatttgcc taatagattt gatcgagtac aatacccatg
    12781 atctttagct a
//
LOCUS       DASRTJ010000724         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_214190,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000724 DASRTJ010000000
VERSION     DASRTJ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214190"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF70_14665"
     CDS             complement(<1..255)
                     /locus_tag="VFF70_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45978.1"
                     /translation="MNRKRVISVVMIVGMLLAACAPPAVQPTTPPAANSTAAPAKVLH
                     IGYVLHGLNNFTQVIKQGAEDAGRALGVDVEVTGPAGFVSN"
     gene            complement(496..1494)
                     /locus_tag="VFF70_14670"
     CDS             complement(496..1494)
                     /locus_tag="VFF70_14670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY45979.1"
                     /translation="MTQSPQTESLTNATPTLLGRLGRRISRVEEIGVIGALLIMMIAL
                     SVTTTTFLTPTNLLQVARQASYFGIMAIGMVFVMSMGDIDLSVGSILMVTNIVAALAL
                     RDGQPVLVAILIGLATGALCGFVNGVLSVILRIPTIIVTLGTLSIYRGLGLVISNAAP
                     ISKFPKDNWFFTVAGGKILDVPSSVILMLSVAVLGYILYNQTAFGRRIQAIGSNLQAA
                     RFSGIRIARHRILAMTLMGTISALAGLAALAFLQSADPTTGQGFELLVIASTIIGGTA
                     LSGGSGSVPGAMIGALIIAMIRNGLVLLGASAYWGLAATGAVIIAAVALDYFIKRR"
     gene            complement(1567..2589)
                     /locus_tag="VFF70_14675"
     CDS             complement(1567..2589)
                     /locus_tag="VFF70_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZY45980.1"
                     /translation="MANLKFAVFGTGFWSQFQIPAWFEVGGVDLVALYNRTVSKAEVI
                     AQRFNVPRVYGDPEELLKNEQLDFIDIITEVPFHAPLVYLAAKYKIPVICQKPMAPDY
                     ETAQKMVQVCRDANVPYMIHENFRWQTPMRALKQAVDSGQIGTPFRARIDFITGFPVF
                     ANQPFLKTLEHLIITDLGSHMLDIARWLFGEANSLYCQTKRVQPDIAGEDAATFILQM
                     GGRTTVVANIAYAGSFVEREVFPQTLVFIEGDKGSIELAPDYWLRVTTKEGTFSRRVP
                     PPRYAWADPAYDAPHASIVPCNANLLNAIKTGQPAETSGEDNLKTMRLVYSSYESAAR
                     NEVIKF"
     gene            complement(2609..2740)
                     /locus_tag="VFF70_14680"
     CDS             complement(2609..2740)
                     /locus_tag="VFF70_14680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45981.1"
                     /translation="MFDQITAGDPEEARRAMREHLAEGEETLRKALGSSISSSENAS"
BASE COUNT          636 a          793 c          744 g          584 t
ORIGIN      
        1 gttcgacacg aagcccgctg gtccggtcac ttccacgtcc acacccagcg cccggcctgc
       61 atcctcggcg ccctgcttga tcacttgcgt gaagttgttc aagccgtgca gcacatagcc
      121 aatgtgcaac actttcgccg gggcagccgt cgaattagcg gctggcggag tcgtcggctg
      181 aacggctggc ggagcacaag cagcgagcaa catacctaca atcatcacaa ctgagataac
      241 acgcttgcgg ttcattgatc taatcctcct aggatagagt gttatgcatt cgggcgatat
      301 gccgggatcg ttctcggaaa ttcgcccacc gattgagaca ccgagaattc atggctagct
      361 gtagttcaat ttctgtatcc gtccaatcac ctccttcccg tccaagtgcg ctgcgcgctt
      421 cggttcttct ttaatataag tgcaaatggc tcgaaatcat caggtagact ttacggggcg
      481 gcatgtcagc ggccatcaac gccgtttgat gaaatagtca agcgcaaccg ctgcaatgat
      541 gaccgcgcct gtcgcagcca atccccagta agctgaggcg cccagcagca ccagaccgtt
      601 gcggatcatc gcaataatca gcgcaccgat catcgcgccc ggaacggagc ctgaaccacc
      661 actcaaagcc gtgccgccga taatggtcga cgcgataacc agcagctcaa agccttgacc
      721 cgtggttgga tcggcggatt ggagaaacgc tagggcggcg agtcccgcca gcgccgagat
      781 cgtccccatc agcgtcatgg ccaagatccg gtgccgcgca attcggatgc ccgaaaagcg
      841 cgccgcctgc aagttgctgc cgatagcctg gatgcggcgg ccaaacgcgg tctggttgta
      901 caagatatag cccaacacag caacgctgag catgaggatc acgctcgacg gcacatccag
      961 gattttgccg ccggccaccg tgaagaacca attatccttc gggaacttgc tgatgggcgc
     1021 cgcattcgag atgacaaggc cgaggccgcg atagatgctc agcgtaccga gtgtcacgat
     1081 aatcgtcggg atgcgtaaga tcacgctcaa cacgccattc acaaaaccac atagggcgcc
     1141 ggttgctaaa ccgatcagta tggccacgag cacgggttgg ccgtcgcgca aagccagcgc
     1201 tgcgacaatg ttcgtcacca tcaggattga gccgaccgac aggtcaatat cccccattga
     1261 catcacgaag accatcccga tcgccatgat gccgaagtac gaggcttggc gtgccacctg
     1321 caacaagttc gtcggcgtca ggaaagtcgt cgtcgtaacg gacaacgcga tcatcatgat
     1381 cagcaaggcg ccaatcacac caatctcttc gacacgactg atgcgtcgtc caagcctgcc
     1441 cagcaaggta ggcgttgcat tcgtgaggct ttctgtctgc ggtgattgcg tcatgcgtca
     1501 ctccaggtat ttggataatc gtttggcaca agcggcacgg ctcagttcgt cgtagccatc
     1561 tggaaatcag aatttgatca cttcattgcg cgcggcagat tcataagacg aatacaccag
     1621 acgcatcgtc ttgagattgt cctcaccgga tgtctcggcc ggttggccgg tcttgatggc
     1681 attcagcaag ttggcattgc acgggacaat gctggcgtgg ggagcgtcgt aagcgggatc
     1741 ggcccatgca tagcgcggcg gcggcacgcg tcgggagaag gtgccttcct tcgtcgtgac
     1801 gcgcagccag taatccggcg ccaactcaat cgagccttta tccccctcga taaaaaccag
     1861 ggtttgcggg aacacttctc gctcgacgaa actacccgca taagcgatat tagccacgac
     1921 ggtcgtccgg ccacccatct gcagtataaa tgtcgcggca tcttcgcccg caatgtctgg
     1981 ctgcacgcgc ttggtttggc aatacaggct gtttgcttcg ccaaacagcc agcgagcaat
     2041 atcaagcatg tggctgccca gatcggtgat gatgaggtgt tcgagtgttt tcaagaatgg
     2101 ttgattggca aaaaccggaa agcctgtgat gaaatcgatg cgggcgcgaa acggcgtacc
     2161 gatctgcccg ctgtccaccg cttgtttcag tgcgcgcatg ggcgtctgcc agcgaaaatt
     2221 ctcatgaatc atgtacggca cgttggcgtc acgacaaacc tgcaccattt tctgcgcggt
     2281 ttcgtaatcc ggagccatcg gcttctgaca aataacaggg atcttatact tcgccgccag
     2341 atacacgaga ggcgcatgaa agggcacttc ggtgataatg tcgatgaaat caagctgctc
     2401 gtttttgagc agttcttccg ggtcaccgta cacgcgcggc acgttgaagc gctgcgcaat
     2461 tacttccgct ttggaaacgg tgcgattgta taaggcgact aggtccacac cacctacctc
     2521 aaaccaagcg ggaatctgaa attgagacca gaagcctgtg ccaaatactg caaacttcaa
     2581 gttagccatg aatgatcctc cgtacaagtt aggatgcatt ttcgctgcta ctgattgatg
     2641 atcccagcgc tttgcgcagt gtctcttcgc cttcagccaa atgctcacgc attgcccgcc
     2701 gcgcctcttc aggatcgccg gctgtgattt ggtcaaagat ggcgcgatgg tatttga
//
LOCUS       DASRTJ010000725         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_375407,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000725 DASRTJ010000000
VERSION     DASRTJ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375407"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1137)
                     /locus_tag="VFF70_14685"
     CDS             complement(295..1137)
                     /locus_tag="VFF70_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZY45982.1"
                     /translation="MFRHTLRRLRGQMIGWGLALAVLGFGIASLFNTVSQMRDQIEQL
                     IGTLPPAVLAFAGNIDRMFSPAGYLDARYFLLMPLILGVFTVVIGSGLIANDEENGTL
                     DLILAQPISRTQLFLGRWLAFLGALIGILFIAWLGLVIAIVLTSIDLNPLQVALPFLS
                     LFGILIWFGSFALMLSLIVPSRRAAASLSGLLLVASYFITTLARVSTDLAVLAKFSPL
                     NYYQGGDALNGLNVVWLAGLLIASIVFTAIAWWWFQRRDIRVFGEGTWGLNLSRIGAK
                     MRSH"
     gene            complement(1143..2057)
                     /locus_tag="VFF70_14690"
     CDS             complement(1143..2057)
                     /locus_tag="VFF70_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY45983.1"
                     /translation="MNQTSAISIQNLTKSYGKVQALRGVNLEVQRGEVFGFLGPNGAG
                     KTTTIRCLLDLIRPSAGSIRVLGIDPQIDPVAVRSRSGYLPGELHVEENLTAEGLMKY
                     FDQLRGNHVDWMFVRQLADRLQLDLKTSFKNMSKGNKQKVGLIQALMHRPELLLLDEP
                     TAGLDPLIQQEALKLIGEARANGTTVFFSSHILSEVQEIADRVGIIRSGQVVEIAATA
                     ALINRSLQRARIKFKQAIDSEALSKLPGVKILARDATSIILQVEGEMDTLIKTLANYP
                     VRDFETERPSLEEIFLAYYEPDQTKAQA"
     gene            complement(2198..>2595)
                     /locus_tag="VFF70_14695"
     CDS             complement(2198..>2595)
                     /locus_tag="VFF70_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HZY45984.1"
                     /translation="AWLPAAQGNVVKVIAALLPYLPPDYNYRVIFMLRAMPEVLASQR
                     QMLVRRGEDPDKIGDDVMARLYEKHLKQVDSWIAGQANVQRLDVNYNEMLTNSQPIVL
                     HVNEFLGGKLNVSKMSEVVDPKLHRQRKE"
BASE COUNT          630 a          705 c          688 g          572 t
ORIGIN      
        1 atctagattc aaacaactcg gctgtgttga ttcagtatat taaattggag aacgaagggt
       61 gggaacgcga ttatgaggtg gttgagccaa gtgaaccctc attcaccgag ccagcgagct
      121 ctaaaaaatc ctgaagcaat ggagaaaggc gcttgcatgg agcggttttt ccggcaattt
      181 gctaatactg catgcacccg acgttaggta tttttgtaaa taggagttga aagatggcca
      241 ccgccgatct ggaacgatcg gcggtggtgt ttagtgggtg aggcccttcc gacatcaatg
      301 cgatcgcatc ttagcgccta tgcgcgataa attcaatccc caagtccctt cgccgaacac
      361 gcgaatatcg cgccgctgga accaccacca agcaatcgct gtgaagacga tcgaggcgat
      421 cagtaatccg gcgagccaaa ccacatttag cccattgagc gcgtcgccgc cttgataata
      481 attgagcggt gaaaacttgg cgagtacagc cagatcggtg ctcacgcggg cgagggtggt
      541 gatgaagtaa ctagcgacta gcagcaagcc actcagcgat gcggcggcgc ggcgcgaggg
      601 aacgatcaaa ctgagcatca gcgcgaaact gccaaaccag atcaagatac cgaagagcga
      661 gaggaacggc agcgcgactt gcagcggatt gagatcgatc gacgtcagga cgatcgcgat
      721 aactaaaccc agccaggcaa tgaacagaat tccgatcaac gcgccgagaa aggccaacca
      781 ccgcccaagg aacaactgtg tgcggctgat aggttgtgcc aggatcagat cgagcgtgcc
      841 gttctcttca tcgttcgcga tcaatccgct gccgatgacg acggtgaaca caccgaggat
      901 gagcggcatc aacaagaagt aacgcgcatc caaatatcca gccggactga acatgcgatc
      961 gatatttcca gcaaaggcca agactgcggg cggcagcgtg ccaatcaact gctcgatctg
     1021 atcgcgcatc tgagacacgg tgttaaataa cgacgcgatg ccgaagccta atacagccag
     1081 agctaacccc cagccgatca tctgtccgcg caggcgtctc aatgtgtgcc gaaacatcgc
     1141 gatcatgctt gtgcctttgt ttgatccggc tcgtaatatg ccagaaaaat ttcttccagc
     1201 gatggccgct ccgtttcaaa atcgcgcact ggataattcg ccagtgtctt aatcagggta
     1261 tccatctcgc cttcaacttg caaaatgatc gacgtcgcat cgcgcgccaa gatcttcacg
     1321 ccgggcaatt tcgataacgc ctcactgtcg atggcttgct tgaatttgat cctcgcccgt
     1381 tgcagcgatc gattgatcag tgcagcggtt gcggcgatct caaccacttg ccccgatcgg
     1441 atgatcccaa cccggtcggc gatctcctgc acttcgctca aaatgtgcga tgaaaagaag
     1501 accgtcgtgc cgttcgctct cgcttcgccg atcaacttca gcgcttcttg ctggatcaac
     1561 ggatcgagtc cggcggtcgg ttcatcgagc agcagcaatt ccggtcgatg cattaacgct
     1621 tgaatgagac cgactttttg tttgttgccc ttcgacatgt ttttgaacga agtcttcaaa
     1681 tccaactgta gccgatcggc caattggcgc acgaacatcc aatcgacgtg attgccgcgc
     1741 agttgatcga aatatttcat caaaccttcc gctgtgagat tttcttcgac gtgcagttcg
     1801 ccgggcaagt aacccgatcg ggatcgcacg gccactggat cgatttgagg atcgatgccc
     1861 agcacgcgaa tcgatccagc gctgggccgg atcagatcaa gcaggcagcg aatcgtcgtc
     1921 gttttacccg caccgttcgg tcccaggaag ccaaatactt cgccgcgctg gacttcgaga
     1981 ttaacgccgc gcagggcttg aactttgcca taagacttgg tcagattttg aatggagatc
     2041 gcggaggttt gattcatagt tatgggcttg cttgtcattc ccgcgtaggc gggaatccag
     2101 agtggaatga ctggacaccc gcttttggta cacccaaagg ggttttacca ccgaagggcg
     2161 cgggtgtggc gcttgcatca ggtgagtgag taaacaatca ttccttccgc tgtcggtgca
     2221 actttggatc gacgacctcg ctcatcttag agacattcaa cttgccgccg agaaattcat
     2281 tgacgtgaag aacgatcggc tgtgagttcg ttagcatttc gttgtaattc acatccaggc
     2341 gctggacgtt tgcctgccct gcgatccagc tatcgacttg cttgagatgc ttctcatata
     2401 acctcgccat cacgtcatcg ccgattttat ccgggtcttc accgcgccgg accaacatct
     2461 gtcgctgcga ggcaagcact tccggcatcg cgcgaagcat gaagatcacg cgatagttgt
     2521 aatcgggcgg cagatacggc agtagcgcgg cgatcacttt caccacattg ccttgcgcag
     2581 caggcagcca cgcaa
//
LOCUS       DASRTJ010000726         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_176748,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000726 DASRTJ010000000
VERSION     DASRTJ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176748"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFF70_14700"
     CDS             complement(<1..902)
                     /locus_tag="VFF70_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HZY45985.1"
                     /translation="MSNPTDATIQKIHQRVKDQHDAIVQFMRDICATPSMDSQLGAVG
                     ERIGAEMKKLGFDEVRFDKMGNILGRIGSGPTKIVYDSHIDTVGIGDRSTWEWDPFEG
                     KVEDGILYARGSCDEKGSTPGMIYGLALARDLGLLDRFTAYYFGNMEEWCDGIAPNSF
                     VEVDPKVRPDFVVIGEPTKMRIYRGHKGRIELKITADGRSAHAASHQLGDNAVYKMMN
                     IISAIRELDRRLPTDPFLGKATIAVTDVQARTASLNAVPDQFTIYLDRRIIFGETKEF
                     SLSQIEGLIPDYLRDEIHVEKLFY"
     gene            complement(1062..1442)
                     /locus_tag="VFF70_14705"
     CDS             complement(1062..1442)
                     /locus_tag="VFF70_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZY45986.1"
                     /translation="MASMFKNVNVVYHYVTDWERGKKFYSDLLEWPVAYESDEAGWME
                     WGMDTMTHVAINKWDEPGPAPIGRGGTLTLTVADVYKTVEALKMRGIKVDEVLHIPNV
                     VLIGTAYDPEGNRIQFVQEGPPPA"
     gene            1660..3321
                     /gene="murJ"
                     /locus_tag="VFF70_14710"
     CDS             1660..3321
                     /gene="murJ"
                     /locus_tag="VFF70_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZY45987.1"
                     /translation="MDSSSDPILIPAADASHDAITFEPEKLSEVERDAVGGGIARAAG
                     IVSLGNVLSSILGLARETVKSHLFGASGAVSAYNVAAILPSQLYDLLVGGLVNSALVP
                     IFSEVAAKKDRAELWRLLSLIFSVAIVFLSIAILAIELLAPQVARLMAGGFDESLLRT
                     TTDLLRITIPAVLFLSLSGVLAGLLYALKRFAYPAFAAPLYNASMIVVAIILADRFGI
                     AAMAIGLLVGSIAQVIVQVPALRGATIRWRFDLRDPILRQMIRLYIPVAGGVIIGQIA
                     ILLSLNLASRTGASGIAWMDYPTRLIQFPLGLIVSAVSIAILPALSRHAAQNSPSDFK
                     MTLAQGLRLVLVLIIPSTIGMAVLAPHIVQLLFQHGAFTAFDTQAVSIMLWLYLIGLI
                     FAGLDQPLVFAFYARKDTLTPALVGLICNVFIYLIAALAPSIISHRPLQVTDLAIANS
                     IQWTSHALIMLWLVRRRMGGLHGFGLGRLVVKAVAGSIAMGAAVWLVISAITNFVGEA
                     SVSREAIGVIGAAIIGMIIYIAAMSVMRADELKSIWRIVVKRRVA"
     gene            3535..4335
                     /locus_tag="VFF70_14715"
     CDS             3535..4335
                     /locus_tag="VFF70_14715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY45988.1"
                     /translation="MNSELQKPSLPSTLYTEDYFLNACEGYEEFVESQGERLSRRLTE
                     AFAYAEVKPGMQVLDVGCGRGEILRHVTKLGARAYGIDYAEVAVKLSREATKDEATPI
                     GVYRTDAKRLPYPDNMFDRALMFDVVEHLFPWELNASLSDIYRILKPGGMIVVHTAPN
                     RWYDRYAYPFVRFIRRLQGKKYPANPRALNVEANVEVHVNEQDIRSMKRYLRRAGFHH
                     VKVWLNSPPQNRHENILFRAARHIAFEWLPFRWFFEREVFAVGVKNDK"
BASE COUNT          901 a         1177 c         1187 g         1083 t
ORIGIN      
        1 tcgtaaaaca atttctcgac gtgaatttcg tcgcgcagat aatcggggat caagccctcg
       61 atttgagaca gcgaaaactc tttggtttcg ccgaagatga tccggcgatc aaggtagatc
      121 gtgaattgat ccggcaccgc attcaatgac gccgtccgcg cctgcacatc cgttacggca
      181 atcgtcgcct tgccgagaaa tggatcggtg ggcaaacgcc gatcgagttc gcggatcgcg
      241 gagataatgt tcatcatctt ataaaccgcg ttgtcaccca gttggtggga ggccgcatgc
      301 gcggatcggc catctgcggt gatcttcaat tcgatgcggc ctttgtgccc gcgataaatc
      361 cgcatcttgg ttggctcgcc aatgacgacg aaatcgggcc gcaccttggg atcgacttcg
      421 acgaaggaat tgggcgcgat gccatcgcac cattcttcca tgttgccgaa ataataagcg
      481 gtaaacctat cgagcagacc gagatcgcgc gcgagcgcca aaccatagat catgccgggc
      541 gtgctgcctt tttcatcgca cgaaccgcgc gcatataaaa taccatcttc gactttgcct
      601 tcaaacggat cccactccca cgtcgatcga tcgccgatgc cgacggtatc gatgtgcgag
      661 tcgtaaacga tcttcgttgg tccgctgccg atgcgcccca gaatgttgcc catcttgtcg
      721 aagcggactt cgtcgaagcc cagttttttc atttccgcgc caatccgttc gccgaccgcg
      781 ccgagctgcg aatccatcga cggggtggcg cagatgtcgc gcatgaattg cacaatggcg
      841 tcgtgttgat ctttcacgcg ctggtggatc ttttggattg ttgcatcggt cggattgctc
      901 atcttggctg cctctttgtt cgattggatt gtttgcggag aatgactgcg cggagaatct
      961 taacacccga tcgatcgatc gtcaaacaaa cgtaggggca ggtttcaaac ctgcccctac
     1021 caagtgattt gtaatgatgc gtgtaaattc gtgattcgaa attacgccgg aggcggacct
     1081 tcctgcacga attgaatccg attgccttcc ggatcatacg ccgtgccgat aagcacgaca
     1141 ttcggaatgt gcagcacttc atcgaccttg atcccgcgca tcttcaaggc ctcgacggtc
     1201 ttgtagacat ccgcgacggt caacgtcagg gtgccgccgc ggccgatcgg ggcggggccg
     1261 ggttcgtccc atttgttgat tgccacgtgt gtcatggtgt ccatgcccca ctccatccag
     1321 ccggcttcat cgctttcata agcgacaggc cactccagca aatcgctgta aaactttttg
     1381 ccgcgttccc aatcggtgac atagtgataa accacattca cgttcttgaa catcgatgcc
     1441 atgcttcacc tccccttgag attttgaaat aggctaatag catataagaa tacacagtcg
     1501 ttgtcaatag acggcagatt aaataattag ggatgtcatt ccgagctgag cgaggaatct
     1561 ctacttctgc taacaatatt acttatcaga gtcagagatt cctcatcgct acgcgattcg
     1621 gaatgacaaa acgatattct ggggtacaat cgcgtcgcta tggattcttc atctgatccc
     1681 attctcattc cggcggccga cgcttcgcac gatgcgataa ccttcgagcc ggaaaaatta
     1741 tccgaagtcg aacgcgacgc cgtcggcggc ggcatcgcgc gcgcggcggg catcgtctcg
     1801 ctcggcaatg tgttgagcag catcttggga cttgcccgcg aaacggtcaa gagccatctc
     1861 ttcggcgcat cgggcgcggt tagcgcgtac aacgtcgcgg cgattttgcc gtcgcagtta
     1921 tacgatctgc tcgtcggcgg attggtgaac tccgctttag tgccgatctt ctccgaagtc
     1981 gccgccaaaa aagatcgagc cgaattgtgg cgcttgctca gtttgatttt ctccgtagcg
     2041 attgtctttc tctcgatcgc gatcctggcg atcgagttgc ttgcgcctca agtcgcccga
     2101 ttgatggccg gcggcttcga cgaatctctg ctgcgaacca cgaccgacct gttgcgcatc
     2161 actattcccg ctgttttgtt tctcagttta tccggcgtct tagcgggctt gttatacgcg
     2221 ctcaaacgct ttgcttatcc ggcttttgcc gcgccgttgt ataacgcctc gatgatcgtc
     2281 gtggcgatca ttctggccga tcgatttggc atcgcggcga tggcgatcgg gttgctcgtc
     2341 ggctcgatcg cgcaagtgat cgttcaagtg cccgctctgc gcggcgcgac gattcgctgg
     2401 cggttcgatc tgcgcgatcc gattttacgg cagatgatcc ggttgtatat tccggtcgca
     2461 ggcggcgtga tcatcggcca aatcgcgatc ttgcttagtt tgaatctagc gtcgcggacg
     2521 ggcgcatcgg gcatcgcgtg gatggattat ccgacgcggt taatccagtt tcccctcggc
     2581 ctgatcgtct cagcggtttc gattgcgatc ctgcccgctt tatctagaca tgcggcccag
     2641 aattccccca gcgactttaa gatgacattg gcgcaaggac tacgcttagt attagtgttg
     2701 atcattccgt cgacgattgg tatggctgtg ctcgcaccgc acattgtgca gctgctcttt
     2761 caacatgggg cgttcacagc ctttgatacg caagccgtgt cgatcatgtt gtggctgtat
     2821 cttatcggcc tgatcttcgc cggactcgat cagcccttgg tgtttgcgtt ttacgcgcgc
     2881 aaagacactt taacgcctgc cctcgtcggc ttgatctgca acgtgttcat atacttgatc
     2941 gctgcgctcg cgccatcgat catcagccat cgcccgctgc aagtcaccga cttggcaatc
     3001 gccaactcga tccaatggac aagccacgcg ctgatcatgt tgtggctggt gcgccgacga
     3061 atgggcggct tgcatggttt tggtttgggt cgcttggtag ttaaagcggt tgccggctcg
     3121 atcgcgatgg gcgctgcagt ttggttggtg atttcagcca tcaccaattt tgttggtgaa
     3181 gcatcagtct cgcgtgaagc gatcggcgtc atcggtgcgg cgatcattgg catgatcatc
     3241 tacatcgccg cgatgagcgt catgcgcgcc gacgaactca aatcgatctg gcggatcgtt
     3301 gtcaaacgcc gcgtggcgtg aggatgatcg atcttggtat aatcatcgtg cattatcagt
     3361 gcatcgaacg cggtaggcgc agagatcatt aatgagcaat tgtcaccctg agcgaaggcg
     3421 atgcctagtg gcccatctga gcgcgatgcg ctcagagtat gggcagggtc tcacacaatt
     3481 agccgagagg ctccgcttcg ctcagcatga caatcccaga gaggagactc cttattgaat
     3541 tctgaactac agaaaccttc gctgccttcg accttgtaca cagaagatta ctttctgaac
     3601 gcatgtgaag gttacgaaga atttgtcgaa tcgcaaggtg aacgcttgtc acgccgactt
     3661 accgaagcct tcgcgtacgc cgaagtaaag ccgggcatgc aagtgctcga cgtcggctgc
     3721 gggcgcggag aaattttgcg gcatgtcacc aaactcggcg cgcgggcgta tggcatcgat
     3781 tacgccgaag tcgcggtgaa actcagccgc gaagcgacga aggatgaagc gacgccgatc
     3841 ggcgtgtacc gcaccgatgc caaacgcttg ccgtatcccg acaacatgtt cgatcgcgcg
     3901 ttgatgttcg atgtggtcga gcatttgttt ccgtgggaat tgaatgcgtc attgagtgac
     3961 atctatcgta tattgaaacc gggtggcatg atcgtggtgc ataccgcgcc taatcgctgg
     4021 tacgatcgat acgcgtatcc gttcgtgcgc ttcattcggc gcttgcaggg caagaagtat
     4081 ccggcaaatc cgcgtgcgct caacgtcgaa gccaatgtcg aagtgcatgt gaatgaacaa
     4141 gacattcgca gtatgaagcg atacttgcgc cgcgccggct ttcaccatgt caaagtgtgg
     4201 ctgaacagcc cgccgcaaaa tcgccacgaa aatattttgt ttcgagcagc acgtcatatc
     4261 gcctttgagt ggcttccgtt tcgctggttc tttgagcgcg aagtgtttgc agtaggagtc
     4321 aagaatgata agtgacgggt gattcgtt
//
LOCUS       DASRTJ010000727         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_214408,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000727 DASRTJ010000000
VERSION     DASRTJ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214408"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFF70_14720"
     CDS             complement(<1..485)
                     /locus_tag="VFF70_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZY45989.1"
                     /translation="MRRNLFRLMVLSIVTALIVSACGTSSEPTKGDLIVYVAAPQSGF
                     QANGGQTVLGGVRLAADEFNRSGGLLGYKIVIVPLDDESDDGVAVDMANQVKADLAAG
                     KRVLGVIGHYNSGQTIAAMEIYKDLPIVILTPTASEQTLTQKGYTNFFRINANDATQA
                     R"
     gene            complement(607..1512)
                     /locus_tag="VFF70_14725"
     CDS             complement(607..1512)
                     /locus_tag="VFF70_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZY45990.1"
                     /translation="MDPVLLVCLGAVFLVGLVLIMSAIRIVPEFQRLVVFRFGKCIGQ
                     KGPGLVLLYPFGIDRAIMADLREEVREVPHQTSITKDNAPIGIDFLIYWQVVDAVMSV
                     VAVNNFRQAALGIATTTLRAVVGDIALDDVLAQREHINNVLRAKLDEVTERWGVKVTA
                     VEIREIVPPREVQDAMTRQMSAERTRRAVVTEALGTREAAVTVAEGNKQAAILAAEGN
                     KQAAILESEGQRSAQALKAEGFAVALDKIFESAKMIDAKTMTLQYLEALKVLGAGAST
                     KFIFPLEFVNMLKPLIGMSDEAMKK"
     gene            complement(1611..2237)
                     /locus_tag="VFF70_14730"
     CDS             complement(1611..2237)
                     /locus_tag="VFF70_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZY45991.1"
                     /translation="MDLGTQLWQLITNPNVSYILLIAGLLMLLVAVTTPGTGAAEVTA
                     IIALALSAIGLIDLSANFAGVLLVIVGFVVFAIDAAAINHGALTLGGAVAILIGSLLI
                     FPEREGVPGLNGLLIAGVTVSMAGFSVLFLSAFMRMRQQKGVGLAAQNVVGVRGVMKT
                     GVTPPASGTAQIGGQLWTISAEEPIGSGVDVEVIDRQGLTLRVKKINP"
     gene            complement(2300..2866)
                     /locus_tag="VFF70_14735"
     CDS             complement(2300..2866)
                     /locus_tag="VFF70_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45992.1"
                     /translation="MLDMTTDLNNYLAPNERILWQGQGQRKFFSATNPGILFALIFGG
                     VAAVMLVVFLTIGSSTRSSSSVPSFFVVFIPLLFIVIGLGVGIPMSAMSRGANARYVL
                     TSAAAIIVNDSRWTGKRTTIIPLKNIAQLSLSENRDGSGTITFGSNPFAAYGRYGTSM
                     VASATPAFWNIEKPLEVYQLIRKQMTDQ"
     gene            complement(2893..>3606)
                     /locus_tag="VFF70_14740"
     CDS             complement(2893..>3606)
                     /locus_tag="VFF70_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45993.1"
                     /translation="ILLGFFGPDLIPGIQGWYFLLGGAAAWGALTFSIIKDPEMAAQV
                     AAGLLRQKFEPTTLRSPDSRDRLIKAFEYRGRIAEAIEHSQPGVLRDHLKETSNQIDD
                     WIANLFSVAKRLDAFEGDRTIQQDVRSVPQAIQTTQARLSRATDPALRAQIDATLQSQ
                     QSQWTSLQNLESTIERGKLQMDATLSSMGTIYTQLLLMDAKDIDSGRAQRLHDDITEQ
                     VQSLHEVVAAMDEVYQYKK"
BASE COUNT          795 a         1129 c          915 g          767 t
ORIGIN      
        1 atccgcgcct gggtcgcgtc gttcgcgttg atgcggaaga aattcgtgta acctttttgc
       61 gtcaacgttt gctcactggc cgtcggggtc agaataacga tcgggaggtc tttataaatc
      121 tccatcgcgg cgatcgtctg gccgctgttg taatgtccga tcacacccag cacgcgtttg
      181 cccgcagcga gatcggcctt cacttggttc gccatatcga cggccacgcc atcatcgctc
      241 tcatcatcga gcggcacgat cacgattttg tagcccagca gtccgccgga tcgattaaac
      301 tcgtcggccg ccaagcgtac tccacccagc accgtctgtc cgccgttggc ttgaaaaccg
      361 gactgcggcg cggcgacgta gacgatcaga tcgcctttgg tcggttcgct cgatgtgccg
      421 caggccgata cgatcaacgc cgtaacgatc gacagcacca tcaatcgaaa taaatttcga
      481 cgcatcttgt tccctcaacg taggggcggg gcattcgcac acagtgccgt ttcggccaga
      541 aacacttgcg cgatatgcat cacagccgct agcgctcgat tgacgaatgc ctcgccccta
      601 cacaatttac ttcttcatcg cctcgtcgct cataccgatc aacggcttga gcatgttgac
      661 gaattcaagc gggaagatga acttggtgga tgcgcccgcg ccgagcactt tgagcgcttc
      721 aagatattgc aaggtcatcg tcttggcatc gatcatcttc gccgactcaa agatcttatc
      781 caacgcaacg gcgaaacctt ccgccttcaa cgcctgcgcc gatcgctgcc cttccgattc
      841 gaggatcgcc gcttgcttgt tgccctcagc ggcgagaatc gcagcctgct tgttaccttc
      901 agccaccgtc accgctgctt cacgcgtgcc taacgcttcc gtcacgacgg cgcgtcgagt
      961 tcgctcggcg ctcatctggc gcgtcatcgc atcctgcact tcgcgcggcg gaacgatctc
     1021 gcggatttca actgcggtca ctttcacacc ccagcgctcg gtgacttcat ccaacttcgc
     1081 gcgcagcaca ttattgatgt gttcgcgctg cgccagtaca tcgtccaacg cgatgtcgcc
     1141 gacgacggcg cgcaaagtcg tggtcgcaat accgagcgcc gcctgccgga agttgttgac
     1201 ggccaccaca ctcatcaccg cgtcgaccac ctgccaatag atcaagaagt cgatgccgat
     1261 cggcgcgttg tctttggtga tgctggtctg atgcggcact tcgcgcacct cctcgcgcag
     1321 gtcagccata atcgcccgat cgatgccgaa cggatagagc agcaccagcc ccggcccttt
     1381 ctggccaatg cacttgccaa agcgaaacac caccagccgc tgaaattccg gcacgatgcg
     1441 aatggcgctc atgatcaaca ccagaccgac cagaaaaacc gcgcctaaac acactaacaa
     1501 tacgggatcc atacttatgc ctcctgagaa aatgattagt gacgagtgat tagtaaatag
     1561 gaatgagtca ttggaaatcg aatcactcat tactaaccac taattacttt ttatggattg
     1621 atcttcttta cgcgcagcgt tagtccctga cgatcgatca cttctacgtc cacgcccgac
     1681 ccgatcggtt cttcggcgct gatcgtccac agctgcccgc cgatctgcgc cgtgcccgag
     1741 gcgggcgggg tcacacccgt tttcatcacg ccgcgcacac cgacgacatt ctgggcggcc
     1801 aaaccgacac ctttttgctg ccgcatccgc atgaaggcgg acaggaacag cacactgaag
     1861 ccggccatgc tcaccgtcac acccgcgatc agcaacccgt tcaagcccgg cacgccttca
     1921 cgctctggaa agatcagcag cgatccaatc aagatcgcca ctgcgccacc cagcgtcaag
     1981 gcaccgtggt taatggccgc cgcatcgatc gcgaacacga caaagccgac aatgactaac
     2041 aagacgccgg caaaattcgc ggacaggtcg atcaagccaa tcgccgacaa ggccagcgcg
     2101 atgatcgccg tgacttcggc ggcacccgtg cccggcgttg ttaccgcgac gagcagcatt
     2161 agcaggccgg cgatcaagag gatatacgag acattcggat tggtgatgag ctgccacaat
     2221 tgtgttccta gatccatggt gaacctccac ttctaacttt caagttgctc gatatagctc
     2281 aagttgcatt tgtcactcgc tattgatcag tcatttgctt gcgaatcagt tgatacactt
     2341 ccagcggctt ctcgatattc caaaaggcgg gcgtggcact cgcaaccatg ctagtgccat
     2401 agcggccata cgccgcgaac ggattcgagc cgaacgtgat cgtgccgctg ccgtctcgat
     2461 tttcggacaa cgacagctgc gcgatattct tcaatggaat aatcgtcgtg cgcttgcctg
     2521 tccagcggct gtcattcaca atgatcgccg cggccgaagt gagaacatag cgcgcattcg
     2581 ctccgcggct catcgcgctc atcggaatac cgacacccag gccgataacg atgaagagca
     2641 gcgggatgaa gacgacgaaa aacgacggga cagaactact tgatcgagta gatgatccga
     2701 tggtcagaaa aacgacgagc atcaccgccg ccactccgcc aaaaatcaaa gcaaacaaaa
     2761 tacccggatt cgtcgcggaa aaaaatttcc gctggccctg gccttgccac aaaattcgct
     2821 cgttcggggc aaggtaattg ttcaagtcag tcgtcatgtc gagcatgtta ttcatccttc
     2881 agccttccgc cttcattttt tgtattggta cacttcgtcc atcgccgcca cgacttcatg
     2941 caagctctgc acttgttcgg tgatgtcgtc atgcaatcgc tgcgcgcgcc cgctgtcgat
     3001 gtccttcgcg tccatcaaca acagttgagt atagatcgtc cccatggaag aaagcgtggc
     3061 atccatctgc agcttgccgc gctcgatcgt actctccaga ttttgcagcg acgtccactg
     3121 cgattgctgt gattgcagcg tcgcatcgat ctgcgcgcgc agagccggat cagtcgcgcg
     3181 gctcaatcgg gcttgcgtgg tttggatcgc ctgcggcacg gatcgcacat cctgctgaat
     3241 cgttcgatca ccttcaaaag catccagtcg cttcgccacc gaaaacaggt tggcgatcca
     3301 atcatcgatc tgattcgacg tttcctttaa gtgatcgcgc agcacgccgg gctggctgtg
     3361 ctcaatcgct tcggcgatgc ggccgcgata ttcaaacgcc ttgatcagcc gatctctgga
     3421 gtccgggctg cgcagcgtgg tcggctcgaa tttttgccgc agcaaaccgg ccgccacctg
     3481 cgcggccatc tccggatctt tgatgatgct aaacgtcaac gcgccccacg ccgccgcgcc
     3541 gcccagcaaa aaataccagc cttgaatgcc gggaatgaga tcggggccaa agaagcccag
     3601 caagat
//
LOCUS       DASRTJ010000728         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_300939,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000728 DASRTJ010000000
VERSION     DASRTJ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300939"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFF70_14745"
     CDS             complement(<1..534)
                     /locus_tag="VFF70_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45994.1"
                     /translation="MNRINGWIFDVYADVDGMSVWIIDLDGRSHQLRDTLAPSFFVYG
                     SNAELHAVCVLLTSTQAPVRLRHAERYDLFLRRNLVLLEVQVLKPNLFSWIFKRVQNF
                     KPELTFYNADLSPAQFYYFEKNLFPLAYCEIEADEAGRIVGIEAQDSRWDLDYTLPPL
                     KIMSLRLEGESQNPNHGF"
     gene            complement(506..796)
                     /locus_tag="VFF70_14750"
     CDS             complement(506..796)
                     /locus_tag="VFF70_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45995.1"
                     /translation="MGRTNETATQLIRNQQQELTNFFTALRKADQEAFQDLFALAQLH
                     IAPLAYSGAADPQLIMYFAMLLEQHKLILRLQKIINYLLEQQGIESDQWLDL"
     gene            complement(780..1343)
                     /locus_tag="VFF70_14755"
     CDS             complement(780..1343)
                     /locus_tag="VFF70_14755"
                     /inference="COORDINATES: protein motif:HMM:NF020014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY45996.1"
                     /translation="MDTGLALIYGIDISGELIFKPIVQLAAHDRSIFVVDGENSFRSY
                     RIARYAREMKLDSRTVLSNVRVSRAFTCYQLTELIVKLSRLNIDQCAGIVCLGLLGTF
                     YDEDVTLAEARRLLHEAIVQLKSLAERVPVLITVRPPSVKVKDRLSLVNMLMQHVDEV
                     RVLPSAERPSSQLQLQLNAGRIDGPHQ"
     gene            complement(1502..1828)
                     /locus_tag="VFF70_14760"
     CDS             complement(1502..1828)
                     /locus_tag="VFF70_14760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZY45997.1"
                     /translation="MSLGKRLKQLLIGQHNRSAKRTKPRTLEASGSGHAIILPALTPR
                     EKEVVQLIRQGYSNKQVANELGISVSTVKWNMTNILAKFEVESSKQLIALLSNQNADR
                     DWKIIG"
BASE COUNT          464 a          507 c          531 g          463 t
ORIGIN      
        1 aaagccgtga ttcggattct gtgactcgcc ttccaatcgc agcgacatga ttttcagcgg
       61 cggcaaagtg taatcgagat cccagcgtga atcttgggcc tcaatcccga cgattcgtcc
      121 ggcctcatcg gcctcaattt cgcagtaagc gagggggaac aaatttttct caaagtaata
      181 aaattgtgct ggcgacagat cggcgttata gaaagtgagt tcgggcttga agttttgcac
      241 gcgcttgaaa atccacgaga acaaattggg ctttaagacc tgcacttcga gcagcacgag
      301 attgcggcgt aaaaaaagat catagcgttc ggcgtggcgc aggcgaacgg gcgcctgcgt
      361 cgaagtcagc agcacgcaga cggcatgcag ttccgcattt gaaccatata caaagaacga
      421 tggcgcaagc gtatcgcgca gttggtgcga tcggccatcg aggtcgatga tccacacgct
      481 cattccatcc acgtcggcgt agacatcaaa gatccagcca ttgatccgat tcaatgcctt
      541 gctgctccag taggtagttg atgatcttct gcaaccgcag gatcagtttg tgctgctcca
      601 gcagcatggc gaagtacatg atcagctgcg gatcggccgc gccggaataa gccagcggcg
      661 cgatgtgcag ctgcgccagc gcgaataaat cctggaaggc ctcttgatcg gctttgcgca
      721 gcgcggtaaa aaagttggtc agttcctgct gctggtttcg aattagctgc gtcgcggttt
      781 cattggtgcg gcccatcgat tctcccggca ttcaattgca gctgcagctg gctcgatggt
      841 ctctccgctg atggcagcac acggacttca tcaacatgct gcattaacat attcaccagg
      901 ctgagccggt ctttcacttt gacggatggc ggacgaacgg tgatcaaaac cggtactcgt
      961 tcagctaacg acttaagttg cacgatcgcc tcatgaagca agcgccgtgc ttcggccagc
     1021 gtcacatctt catcgtaaaa agttccgagc aggcccagac aaacgatccc ggcgcattga
     1081 tcgatattca ggcggctcag cttaacgatc agttcggtta attgataaca ggtgaaggcc
     1141 cgcgacacgc gcacatttga cagcacagtg cgtgaatcaa gcttcatctc gcgggcatac
     1201 cgcgcgattc gatacgagcg aaacgaattc tcgccatcga ccacgaagat ggatcgatca
     1261 tgggcggcca gctgcacgat cggcttgaag atcagctcgc cggaaatatc gatcccataa
     1321 ataagcgcga ggcctgtatc catgcgctca atgtatcacg cgccgagcgg cgttgcaccc
     1381 agcttgggta tggaaaaggt tggggattgg cctgtctgaa ggtcggggtc gaaggaaaac
     1441 gttcgcggaa aataaaaaat ccgccgaggt tgacggatta gatagaggca aagaaattta
     1501 actagccgat gatcttccag tcgcgatcgg cgttttgatt ggacagcagc gcgatcaatt
     1561 gtttgctgga ttccacttcg aacttcgcca aaatgttggt catgttccac ttgacggtcg
     1621 agaccgagat acccaattca ttcgcgactt gtttgttgga ataaccttgc cgtatgagct
     1681 gcacgacttc tttttcgcgc ggcgtgaggg cgggcaatat gatggcgtgc cccgaccccg
     1741 acgcttccag cgttcgcggt tttgttctct tggctgatcg attgtgctga ccgatcagta
     1801 attgtttaag ccgtttgccc agtgacatca tcacatccga tttgttttac tgctgggcgg
     1861 caaatttctt tgcttcttcc gccagctcgg ttctctgcct caaaacgggc gggaaactta
     1921 cggtaaccaa tattccaatt gaatattacc cgggtcgcgg aaggt
//
LOCUS       DASRTJ010000729         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_148200,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000729 DASRTJ010000000
VERSION     DASRTJ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148200"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFF70_14765"
     CDS             complement(<1..688)
                     /locus_tag="VFF70_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZY45998.1"
                     /translation="MKAILFHQHGNAEVLQYEDFPTPQPGPGQVLVQLKASALNRADI
                     WTRGGYPGLKLDMPHILGADGAGVIASIGEGVTQFKVSDRVVINSNLSDGTCEFCIAG
                     QDNLCVKWNLLGETVRGTYAEYIALSDRNVLPIPTDFPFDRAAAAALVFHTAWHSLIV
                     RGGLQAGESVLIVGAGGGVNTASIQIAKYAGAKVYVVASDAAKADRAKELGADVVIDR
                     SKEDWPRSIFQ"
     gene            990..>2738
                     /locus_tag="VFF70_14770"
     CDS             990..>2738
                     /locus_tag="VFF70_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZY45999.1"
                     /translation="MLPNFTQFIDLLTGSGALIYHLIVLFTLEAIIGMTIGHARRNGW
                     SPALRRAMIAAIVLLIGRAILMVVALLGQIGIPPNIIVSSAITPPLERYIDLVSLGFL
                     AWVFVPLLLDRAQLGLGFVLGNLVLATIVYATFGLQWYNTVITPNLFYNTGSQETVWQ
                     AWSLALAILATLGSFVGREESSGITVTAFALLSIGHLLQLISPVSTSHIAGWVWLAQL
                     ATYPLFAIAIYRRVAFGETRDETNLAPTGFDQPSNDTPILEAAQRVANRKDPQLSLIQ
                     ATTALANAMSADLAAIGLPSLQPGSVELAAIYSPASTPANNPAFTLDSQPSIKRAITR
                     RRPVAIMPGEDQPEMAELFTLMGSHVTGPLLIVPLVDDRVVLGVALFGNPTSNRAWTA
                     ADALRARSLCDYLATALTTAKRHETFEHRIDELGGTLRQQEADAAQRRTWFENALQKV
                     QTEALQASTQSQQQQKRITELAALVELHEEQAKQIGSDHGQWQERVDQLTSERTRLAS
                     ELQEHAQETDRLTQLQSGLEKELKEAQQQIGYLQDALNQRPILAAEPSSILPEEDHQR
                     ELVASLVQELRTPMTSI"
BASE COUNT          588 a          790 c          734 g          626 t
ORIGIN      
        1 actgaaagat cgatctgggc caatcttctt tcgatcgatc gatcaccaca tctgcaccca
       61 actctttcgc ccgatcggct ttcgctgcat ccgacgcgac gacatacact ttcgccccgg
      121 cgtatttcgc aatttggatc gaggctgtgt tcacgccgcc gcctgctccg acgatcaaga
      181 ctgattcacc ggcctgcaag ccacctcgaa cgattaaact gtgccacgcg gtatgaaaga
      241 ctaacgctgc ggccgcagcg cgatcgaatg gaaaatcggt cgggatcggc agcacattcc
      301 gatcggatag cgcgatgtat tctgcatacg tgccgcgcac ggtttcgccc agcaagttcc
      361 acttcacaca caaattatct tgaccggcga tgcagaactc gcacgtgccg tcgctcaaat
      421 tagaattgat tacaacgcga tcgctgactt tgaattgcgt cacgccttca ccgatcgacg
      481 cgatcacgcc cgcgccatct gcgccgagga tgtgcggcat gtccagtttc aagccgggat
      541 aaccgccgcg cgtccaaata tcggcgcgat tcagcgccga ggctttgagc tgcactaaga
      601 cttgaccggg accgggctgc ggcgttggga agtcttcata ctgtaatact tcagcatttc
      661 catgctgatg aaataggatc gctttcatca ttgcgctccg tttgaaattg gttggatgca
      721 attatagtcc atgtgcgtga atgtttcacc gcaagctctc aaccagtaca ttcttaaccc
      781 aattgtcacg cgacccgaat tgctctgatg tgtccaaagt tgtataatca ttctagcatt
      841 gcttaccgtc attgcgagcc gccgaaggcg gcgaagcaat cttctgatcc acattgagat
      901 tgctgcctat tccctgagcg ccctgcgctc aggtgggccg ccaggcgtcg tcggccatat
      961 ggcctcctcg caatcacaac tgaactctta tgctgcctaa cttcactcaa ttcatcgatc
     1021 tgctaaccgg ttccggcgct ctaatctatc atctgatcgt cttgttcacg ttggaagcga
     1081 tcatcggcat gacgatcgga catgcgcggc gaaacggttg gtcgcccgcc ctgcgtcgag
     1141 cgatgatcgc cgcgattgtg ttattgatcg gtcgcgcaat cttgatggtc gttgcgctgc
     1201 ttggacagat cggtattccg ccgaacatca tcgtttccag cgcgatcacg ccgcccttag
     1261 agcgttacat tgatttagtg agcctcggtt tcttggcgtg ggtttttgtg ccgctgctgc
     1321 tcgatcgtgc acagctcggt ttggggtttg tcctcggcaa cttggtgttg gctacgatcg
     1381 tctacgcaac cttcggtttg caatggtaca acactgtgat cacgccgaat ttattttaca
     1441 atacggggtc gcaagaaaca gtttggcagg cgtggtcgct ggctttggca attctggcta
     1501 cactcggatc tttcgtcggg cgagaggaat catcggggat aactgtaacc gcctttgcgt
     1561 tgttatcgat cggtcatctg ctgcaattga tcagtcccgt gtccacttcg cacatcgccg
     1621 gctgggtgtg gctggcgcaa ttggccacgt atccattatt tgcgattgcg atctatcgcc
     1681 gcgtggcctt tggcgaaacg cgcgatgaga ccaacctcgc gcccaccggg ttcgatcaac
     1741 cgtcgaacga cacgccgatt ttggaagccg cgcagcgtgt tgccaaccgc aaagatccac
     1801 aactgtcttt gattcaagcg acgacggctt tggcgaatgc tatgtccgcc gatctcgccg
     1861 cgatcggatt gccgtcactg cagccaggtt cggtcgagtt agccgcgatc tattcgcccg
     1921 cctctacgcc ggccaacaat cccgctttca ctctcgacag ccaaccatcg atcaaacgcg
     1981 ccatcactcg ccgccgaccg gtagcgatca tgcccgggga agatcaacca gagatggctg
     2041 agttgttcac gctgatgggt tcgcacgtca ccggtccatt gttaattgtg ccgctggtcg
     2101 acgaccgagt ggtcttaggc gtggcgcttt tcggcaatcc gacgagcaac cgcgcgtgga
     2161 cggccgcgga tgcgctgcgt gcgcgatcgt tgtgtgatta tttggcgact gccctcacaa
     2221 ccgcgaagcg tcacgaaacg ttcgagcacc gcatcgacga attgggcggc acactgcgcc
     2281 agcaggaggc ggatgcagct caacgccgca cgtggtttga aaacgctttg caaaaagttc
     2341 aaaccgaagc actacaggca tcgacccagt cgcaacagca gcagaagcgc atcactgaat
     2401 tggcggcgct ggtcgagctg cacgaagaac aggccaagca gatcggatca gatcacggac
     2461 agtggcagga acgtgtcgat caattaacca gcgagcgcac gcggctggca agcgaacttc
     2521 aagaacacgc gcaggaaacc gatcggctga cgcagctgca atcggggttg gagaaggaac
     2581 tgaaagaagc gcagcagcag atcggttatc tgcaagatgc gcttaatcaa cgtccgatcc
     2641 tcgccgccga accatcgagc attttgccgg aagaagatca tcagcgtgaa ttggtggctt
     2701 cattagtaca ggaactgcgc acgcccatga cttcgatc
//
LOCUS       DASRTJ010000730         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_148827,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000730 DASRTJ010000000
VERSION     DASRTJ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148827"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFF70_14775"
     CDS             <1..253
                     /locus_tag="VFF70_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY46000.1"
                     /translation="RVREAQRKDEQSAFSQLTDQEMRVMALVAEGKTNREIAQALFLG
                     EGTVRNYVSSILSKLQLTNRAEAAAYAVQHNIKAYGSES"
     gene            complement(362..1711)
                     /gene="miaB"
                     /locus_tag="VFF70_14780"
     CDS             complement(362..1711)
                     /gene="miaB"
                     /locus_tag="VFF70_14780"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZY46001.1"
                     /translation="MCGKIFAAKEQSVMKYHLHIFGCQMNTADAQRVGSDLEKIGLTA
                     TDHASNADVIVLDTCVVRQSAEDKAYSYITTLKPLKEQNPNTIVTVMGCMVGVKGNAA
                     LKKQFPWVDVFMAPSETTPLINYLRQRDEYALLEQETRQRYAIQDGDLILPIHERGNL
                     VAAHVPIVYGCSHACTFCIIPFRRGVERSRPVGQIVQEVRSLVDQGVKEVTLLGQIVD
                     RYGKDAPDGPDLADLLRVIHTVDGLERIRFLTSHPIYMNEKLLDTVAELPKVCEHIEV
                     PVQAGDDHVLDDMKRGYTSDDYRRLIKNIRSKIPNSSIATDIIVGFPGETEARFQKTY
                     DLLEELRLDIAHTAKYSPRPGTVAERRLPDDVPAAEKERRFRAIEEQHQRITAEINAH
                     YQHEWVDVLVEEKHNDRWRGRTRTNKLVFFADDHDRRGQLVKVKIEKTSAWSMTGTI"
     gene            1747..2787
                     /gene="queG"
                     /locus_tag="VFF70_14785"
     CDS             1747..2787
                     /gene="queG"
                     /locus_tag="VFF70_14785"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZY46002.1"
                     /translation="MNLTERIKLRAIELGFDLVGIAPAGVAPHAKEYADWVAAGMLGE
                     MAYMSRDPDRRSDLRRLLPGAQSVIVVGLSHYTLQLSEDVKNDPSRGLIARYAWGVDY
                     HAVMTPRLNDLAKFVRVESSRDVESKVYVDTGAVLERDWAQMAGLGFIGKNTCLINPH
                     MGSWLFLGVLITDLDLEIDQPQKHVSCGNCSRCLVACPTDAFTSPYILDARKCISYLT
                     IELRGSIPIELRSLMSNRIFGCDICQDVCPWPTRFAQPTHERSFFPVDLDRAGPRLID
                     LARLSEDQFKVRFAGTPILRSKRRGLLRNVAVALGNWGSIEAREALQMLSIDPDPIVA
                     EHAEWGLFATGG"
     gene            complement(2798..>3453)
                     /locus_tag="VFF70_14790"
     CDS             complement(2798..>3453)
                     /locus_tag="VFF70_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZY46003.1"
                     /translation="EITFHLLQCRPQSSLREEASQPVPADLPPADRVFISTQMVPHGH
                     VSAIDYLISVDPLTYSQLNDQRKQAVARVVGRLNQTLAGKNFIMLGPGRWGSANIELG
                     VPVTYADIYNSRALVELAFEQGGITPEPSYGTHFFQDLVEAQIYPLAVYPDQSGDYLN
                     REFLGHAHNQLATLLPQDAAYSQCIKVIQIDDERPEYRLEISMNGERALAYLAQKSD"
BASE COUNT          762 a          866 c          958 g          867 t
ORIGIN      
        1 tcgcgtgcgt gaagctcaac gcaaggatga acaatcggct ttcagtcaat tgaccgatca
       61 agaaatgcgt gtgatggcac tagtggccga aggcaaaacg aatcgcgaga tcgcgcaggc
      121 gttgtttttg ggtgaaggca cggtacgtaa ttacgtcagc agtatcttga gtaaattgca
      181 gttgactaat cgtgccgaag cggctgcata tgcggttcag cataatatca aagcgtatgg
      241 ttcagaaagt tagcagttca tcatccatgg cggcgttccg ctggataaac aaaacctccc
      301 gccgatcaac tttgatcggc gggaggtttg tttccgaatt aagcagggtg catcctgcac
      361 cctatattgt ccccgtcata ctccaggcgc ttgtcttctc gatcttaacc ttgaccaact
      421 gaccgcgtcg atcgtggtca tccgcaaaga agaccagctt gttcgtgcga gtgcggccgc
      481 gccaacgatc attgtgcttc tcttcaacca agacgtcaac ccactcatgc tgatagtgcg
      541 cattgatctc ggccgtaatg cgttgatgtt gttcttcgat cgcgcggaag cggcgttctt
      601 tttctgcagc tggaacatcg tccggcaggc gtcgctcggc aaccgtgccc ggtcgcggtg
      661 aatacttggc ggtatgcgcg atatccaatc gaagttcttc gagtagatcg taagtttttt
      721 ggaagcgagc ctcggtctcg cccggaaagc cgacgatgat atcggtggcg atcgaggagt
      781 tcggaatctt tgagcggata tttttgatca aacggcgata atcgtccgag gtatagccac
      841 gcttcatgtc gtccaggaca tgatcgtcgc ccgcctgcac gggcacttcg atatgctcgc
      901 agactttggg aagttcggcg accgtatcca gtaatttctc gttcatgtag atcggatgcg
      961 aggtcaggaa ccggatgcgt tccaggccgt caacggtgtg aatcacgcgc aggagatccg
     1021 cgagatcggg accatcaggc gcatccttgc catatcgatc gacgatctga cccaatagcg
     1081 tcacttcttt cacgccttga tcaacgagcg atcgaacttc ctgcacaatt tgtccgacag
     1141 gccggctgcg ttcaacaccg cgtcgaaacg gaatgatgca gaaagtgcat gcgtgggagc
     1201 agccgtaaac aatcgggaca tgcgcggcca ctaaattgcc gcgctcgtgg attggcagga
     1261 tcagatcacc atcctgaatc gcataacgtt ggcgggtttc ttgttcgagt agtgcatact
     1321 catcgcgctg tcgcaagtaa ttgatcaatg gcgtcgtttc cgagggtgcc atgaagacat
     1381 cgacccacgg gaattgcttc ttgagcgcgg cattgccttt gacaccgacc atacagccca
     1441 tcaccgtgac gatggtgttg gggttctgct ctttcaaagg cttgagggtg gtgatgtaac
     1501 tataagcttt gtcttcagcg ctctgccgca cgacgcacgt atcgagcaca atcacatctg
     1561 cgttgctggc gtgatcggtg gcggtcaatc cgatcttttc aagatcagaa cccacgcgct
     1621 gcgcgtcggc ggtattcatt tggcagccga atatgtggag atgatatttc ataacagact
     1681 gttccttggc tgcaaagatt ttaccacaca tcgatcgggg tgtgaatcat gttaaaataa
     1741 ttggcaatga acctcactga acgcatcaaa ttacgcgcga tcgaattggg attcgatctc
     1801 gtcggcattg cgccagcggg cgtcgcgccg catgctaaag agtatgccga ttgggtcgca
     1861 gccggcatgc tgggtgagat ggcttatatg tcgcgtgatc ccgatcggcg atctgacctt
     1921 cgacggcttt tgcccggcgc gcaaagcgtc attgtggttg ggttgagcca ttacacactt
     1981 caactgtctg aggacgtgaa gaatgatccg tcgcgtggct tgatcgcgcg ttacgcgtgg
     2041 ggtgttgact atcacgctgt gatgacgccg cgcttgaatg atctggccaa gtttgtaaga
     2101 gtagaatcca gccgcgatgt tgaatcgaaa gtctatgttg ataccggtgc tgtccttgag
     2161 cgcgattggg cacagatggc cgggctaggt tttattggca agaatacgtg tttaatcaat
     2221 cctcacatgg gctcatggct attcctcggc gtgttgatta ccgatcttga tctagagatc
     2281 gatcaaccgc agaagcacgt aagttgcggc aactgcagtc gctgcttagt cgcttgcccg
     2341 acggatgctt tcacttcacc ctatatcttg gatgcgcgca aatgtatttc ctatctcacc
     2401 atcgaactgc gcggttcgat tccgattgaa cttcgatcgc tgatgagtaa tcgtatcttt
     2461 ggctgcgata tttgtcagga tgtttgtccg tggccgacgc gttttgctca gccaactcac
     2521 gagcgatcgt tttttccggt cgatctcgat cgtgccgggc ctcgcttgat cgatcttgcc
     2581 cgattgagcg aagatcaatt caaagtgcgt ttcgccggaa cgccaatcct tcgatcgaag
     2641 cggcgagggt tgttgagaaa tgttgccgtc gctttgggga attggggatc gatcgaggcg
     2701 cgggaagctc tgcaaatgct atcgatcgat cccgatccga ttgtggctga acacgcggag
     2761 tgggggcttt ttgcgactgg cggctagcgg ctagacgtta gtcgctcttc tgtgccaaat
     2821 acgctaacgc gcgctcacca ttcatcgaaa tttccaatcg atattccggc cgctcgtcat
     2881 caatctgaat cactttgatg cattggctgt aggcggcgtc ttgaggcaac agcgtcgcga
     2941 gttggttgtg agcgtgaccg agaaattcgc gattgagata atcgcctgac tgatcaggat
     3001 aaacagccag cggatagatt tgcgcttcaa ctaagtcttg aaagaagtgc gtgccgtaag
     3061 atggctcagg ggtaatgcca ccttgttcaa aagccagctc caccagcgcg cgtgaattgt
     3121 aaatgtcagc atacgtcacc ggcacgccaa gttcgatatt agctgatccc cagcgaccag
     3181 gccccaacat gataaagttt tttcctgcca gagtttgatt caagcggccg acgacgcggg
     3241 ccactgcttg tttgcgctga tcatttaact gactataggt taatggatca acgctgatca
     3301 gataatcgat cgcgctgacg tggccgtgcg gcaccatctg cgttgagatg aaaacccgat
     3361 cggctggagg gagatccgct gggaccggtt ggctggcttc ttcgcgcaag ctgctctgag
     3421 gacgacattg caataaatgg aaagtgatct cag
//
LOCUS       DASRTJ010000731         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_148929,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000731 DASRTJ010000000
VERSION     DASRTJ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148929"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFF70_14795"
     CDS             complement(<1..1724)
                     /locus_tag="VFF70_14795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZY46004.1"
                     /translation="MNRLAALLKIRQGEGRTASLLIGLMIFASAGITIGSTGVDALFF
                     ARFGPQNLPYMYLALGVTNFIGMLGVTALIGRVAHHRLFILIPFVFAIVLIIERVVLG
                     LNLPWFMPIVWLLKELMNLVQGTYTWGLAGLFTDTRQAKRLFPLFTAGSIFGAVTGSF
                     ATPLLVNWLNVDNLLIVWAISLAITFILGRILVARINTPSSKPRRSRKQQPSLIQDMQ
                     SGYQFVKRSKLMTWWSIMAIIFSVLWFSIALPFSKAATAQFPNENALASFLATFNGVQ
                     TAVALLLSLFVANRLFARFGLINMLIVFPVIYLLGFAAMTAISIVTIVPFTIFIALVT
                     FKFIQMIYIQGVADTAWQAAFNVVPAQRRDQVRAFINGVPGQAGIFIAGLILIIGENA
                     LQPQWLYLIGLIAAISTVYFAIRSKRAYSTALVDALRDGQPQVFITSDEEPFGGFRKD
                     ATAINAAIDGLSNPDPAIRRVSIEILGRLGVPQATDALVAALRDPDPQVRADALRSLA
                     GAKASAALLEIAACLFDTEPEVRAEAVETLRILAPNPEDLTAYIEPLLHDLMPSVRAR
                     VAAALLSI"
     gene            complement(1815..2858)
                     /locus_tag="VFF70_14800"
     CDS             complement(1815..2858)
                     /locus_tag="VFF70_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZY46005.1"
                     /translation="MISPTSNFQTATSNLQTYFAKYLPAIDDEMRYVVAAPDDPTLKI
                     YYGMLHYHLGWVDQDFEPTRSDAGKRLRPIFVLLSCEASGGEWESALPAAAAIELLHN
                     FSLIHDDIEDGDRTRRGRATLWTIWGQSQAINAGDALFTLSHIALSGMVSRNVPPPRR
                     LAVRERFDLACLALTQGQHLDLSFEARSMIAEDEYLRMIGGKTAALVAGACSIGAIVA
                     GSDQIDQFDRFGHELGYAFQIEDDLLGIWGDPKVTGKAAGNDILHRKKSLPIVYGLTH
                     SVELQHLYGESTIDIEQVIYQLEQLGAREYAESIAAQHHRRALDALDATGANNAAANA
                     LRDLANNLLHRPA"
     gene            complement(2974..>3321)
                     /locus_tag="VFF70_14805"
     CDS             complement(2974..>3321)
                     /locus_tag="VFF70_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZY46006.1"
                     /translation="VELFRAPDDLHKRVAAAFRDWGIPTTESIQMVKRAAPNLPIIAS
                     GGIRSGIDIAKSIALGASIGGMAGPFLKAAAVSVDAVAETLEETIRVLRIAMFAAGTG
                     SIAELQKTPLIKI"
BASE COUNT          794 a          874 c          957 g          696 t
ORIGIN      
        1 tcgatgctta ataaagctgc ggcgacacgc gcgcggaccg acggcatcaa atcgtgaagc
       61 agcggttcaa tgtacgcggt cagatcttcc ggattgggcg cgagaatgcg caaagtttca
      121 accgcttcag cgcgcacttc tggttcagta tcaaacaagc atgcggcaat ttccaacaaa
      181 gcagcggagg ctttagcgcc tgccagcgat cgaagtgcat cggcgcgaac ttgcggatcg
      241 ggatcgcgca gcgcggcgac aagcgcatcg gtcgcttgcg gcacgcccaa tcggcccaga
      301 atttcgatcg agacgcggcg aatagctgga tcggggttcg ataagccatc gatggcggcg
      361 ttaatcgccg ttgcatcttt tcgaaatcca ccgaaaggtt cttcgtcgct ggtgatgaag
      421 acctgcggct ggccgtcgcg cagcgcatcg accaacgcag tactgtatgc gcgtttcgat
      481 cgaatcgcaa aatacactgt ggagattgcg gcgatcaatc ctatcaaata tagccattgt
      541 ggttgcagcg cgttctcgcc gatgatgaga atcaagccgg cgatgaagat accggcttga
      601 ccgggcacgc cgttgatgaa cgcgcgtact tgatcgcgcc gctgagcggg cacgacgttg
      661 aacgcggctt gccacgcagt atcggccacg ccttggatgt aaatcatttg aatgaattta
      721 aaagtgacca gcgcgatgaa gatcgtgaag ggcacgatcg tgacgatcga gatcgcagtc
      781 atcgccgcga agccgagcaa gtagatgact ggaaaaacga tcagcatatt gatcaagccg
      841 aagcgtgcga acaatcgatt cgcgacaaag agcgacaaca acaacgcgac tgctgtctgc
      901 acgccgttaa aagtggcgag gaaactagcg agagcgtttt catttgggaa ctgcgcggta
      961 gccgctttgg aaaacggcag cgcgatggag aaccagagta ccgaaaagat gatcgccatg
     1021 atcgaccacc aggtcatcaa cttcgatcgc ttgacgaatt gatagccgct ctgcatatct
     1081 tgaatcaaac tcggctgctg cttcctcgat cggcgtggtt tgctcgacgg agtgttgatg
     1141 cgcgcgacta ggatgcggcc taagataaaa gtaattgcta gtgagatcgc ccacacgatg
     1201 agcagattat cgacgttgag ccaattcacg agcagcggcg tggcaaacga tccggtgacc
     1261 gcgccgaaga tcgatccggc ggtgaagagt ggaaacaagc gtttcgcttg acgggtatcg
     1321 gtgaagagcc cggccaagcc ccaagtgtat gtgccttgaa ccagattcat taactctttg
     1381 agcagccaga cgatcggcat gaaccacggc agattgagac cgagcactac ccgttcgatg
     1441 atcaacacga tcgcaaatac aaacgggatg aggatgaaca agcgatgatg tgcgacgcgg
     1501 ccgattaacg cggtgacacc caacataccg atgaagttgg tcacgcccag ggcaagatac
     1561 atgtacggca gattttgcgg accgaagcgg gcaaagaaca acgcatcgac gccggtgctg
     1621 ccgatggtga tgcccgccga agcgaagatc atcaagccaa tcaacaacga ggccgtgcga
     1681 ccttcacctt gtcggatttt gaggagtgcg gcgagtcgat tcatgtgatc attccccccg
     1741 aatgatcagc tcaacaatac atccaaacat ttttggacgc ggataaagga tctaatccgc
     1801 atttaaactg aattttaggc tgggcgatgc aacaagttat tcgccaagtc tcttaaggca
     1861 ttcgccgcag cattgttcgc gcccgtcgcg tcgagcgcat cgagtgcgcg tcgatgatgc
     1921 tgcgcagcga tcgattcggc gtattcgcgc gcgccgagtt gttcgagttg ataaatgact
     1981 tgctcaatat cgatcgtcga ttcgccgtac agatgttgca gttcgacgct gtgcgtcaag
     2041 ccatacacga ttggcagcga tttcttccta tgtagaatat cgttgcccgc cgctttacca
     2101 gtgaccttcg gatcgcccca gatgccaagt agatcatctt cgatttgaaa agcatagcca
     2161 agttcatggc cgaagcgatc gaactgatcg atctgatcgc tgccggcgac gatcgcgccg
     2221 atggaacagg cgcccgcaac aagtgccgct gtcttgccgc cgatcatccg caagtattcg
     2281 tcttcggcga tcatcgatcg cgcttcaaaa ctcagatcga ggtgctggcc ttgcgttaga
     2341 gccaggcacg ccagatcgaa gcgttcgcgc accgccagcc gtcgaggcgg tggaacattt
     2401 cggctgacca tgccactcaa ggcaatgtgc gatagggtga agagcgcgtc gccagcattg
     2461 attgcttgcg attgtcccca gatcgtccat agtgttgcac gtccgcgtcg cgtgcgatcg
     2521 ccatcttcga tgtcgtcgtg gatcaatgag aagttgtgca gtagctcgat cgcagccgcg
     2581 gcgggcaggg cggattccca ttcgccgccg gaggcttcac aggataacag cacgaagatc
     2641 ggacgcaggc gtttgcccgc atcagagcgc gtaggctcga agtcttgatc gacccagccg
     2701 agatgatagt gcagcattcc gtaataaatt ttcaacgttg gatcgtcagg cgcggcgacg
     2761 acgtatcgca tctcgtcatc gatggcaggc aggtatttgg cgaagtaggt ctgaagattg
     2821 gaggttgcag tttggaagtt ggaagttgga gagatcatgg tgttcgcatg gtaggggcga
     2881 tgcatgcccc taagaagcgc tgtcgagtga catcgcgtga tggcatgtcc tacgatcggc
     2941 attgacccat acgcgaatgc atcgctctta cgattatatt ttgatcaacg gtgttttttg
     3001 taactctgcg atacttcccg tgcctgcggc gaacatggct atcctcagca cgcggatcgt
     3061 ttcttccaac gtctcggcga cagcatcgac tgaaacggcc gccgctttga ggaacggccc
     3121 ggccatgccg ccgatcgatg cgccgagcgc gatcgatttg gcaatatcaa taccagatcg
     3181 aatgccaccc gatgcaataa tcggcaaatt aggcgcggcg cgtttgacca tctggatcga
     3241 ttcggtcgtg ggaatacccc aatcgcggaa agcggcggca acgcgcttat gcagatcgtc
     3301 gggtgcgcgg aacaattcga c
//
LOCUS       DASRTJ010000732         1933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_214767,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000732 DASRTJ010000000
VERSION     DASRTJ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214767"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFF70_14810"
     CDS             complement(<1..871)
                     /locus_tag="VFF70_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46007.1"
                     /translation="MRKIIALPFGLIFVVLLLATIWVFSLRSFAFDPGFYTTNLKNQG
                     LFQAIQSDPMTYFNWSGSFSQLKALPLDTQRQIFNEAVPPGWIDKEVGAAIQSVMDWL
                     HSDQPPPPHITIDFRPIKDRLQGPPGQQIAADVVALIPECGPNDHPDLSGSSVPSCLP
                     AGLDRSLVTEYVVSVISDAATNIPDTLDVGPDFVGSGRWSSLITGRSWIRLLDVGILL
                     LVAITFLIWFIGALIAGHNFKDGLRWAGGWLLGGSIIVLGLSVLMLIERDAVLSSASF
                     VRSANDNITPATLD"
     gene            complement(885..1877)
                     /locus_tag="VFF70_14815"
     CDS             complement(885..1877)
                     /locus_tag="VFF70_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZY46008.1"
                     /translation="MPTKLAPTIETEYAAAKNGCVLIDSSSRGRLRISGSTRLDFLHR
                     MSTNDLLKLQIGQGAATIFATPIARIIDRTMVYVRENDLIMLTSRDAQAPVMSWLKKY
                     IFFNDDVQIVDATQELGMLSVYGPRAPETFQRVVGIDVSSLGLHHWRSANLGEVEVLI
                     ARADSLGTNGFHLIVQADKLSGLRSKLIDANATPIAEETYQVLRVEAGQPEYGRELTD
                     EIIPLEANLWPDVSFTKGCYTGQEIIARMESRQRLAKQLIGLRLESEINLPARIFADD
                     GDVGSITSIAHSPDRGWLALGYIKAALAIDDQAVQARAGDRSIAAQVVVLPFKF"
BASE COUNT          471 a          547 c          506 g          409 t
ORIGIN      
        1 cgtccagcgt tgcgggcgtg atgttatcgt tggccgatcg gacgaagctg gccgatgaga
       61 gcaccgcatc gcgctcgatc aacatcaaga cgctcaagcc caacacaatg atcgatccgc
      121 cgagcaacca gccgccggcc cagcgcagac catctttgaa attatggccg gcgatcagcg
      181 caccgatgaa ccagattaaa aatgtaatcg ctaccagcag aagaattccg acatccaaca
      241 atcgaatcca acttcgaccc gtgatcaatg agctccaccg gccactgccg acaaagtccg
      301 gcccgacgtc aagcgtatca ggaatgttgg tagccgcgtc actgatcacg ctcacgacgt
      361 actcggttac gagactacga tcgagtccag ccggcaggca ggacggcacg ctgctgccag
      421 acagatcggg atgatcgttt ggaccgcatt cgggaatgag cgctaccaca tcggccgcga
      481 tttgctgacc gggcggacct tgcaatcgat ctttaatcgg gcgaaaatcg atcgtgatgt
      541 gcggtggcgg tggctgatca gaatgcagcc aatccatgac actctgaatc gcagcgccga
      601 cttctttatc gatccagccg ggcggcaccg cctcattaaa aatctggcgt tgtgtgtcga
      661 gcgggagtgc tttgagttgg ctaaacgatc cgctccaatt gaagtaggtc atcggatcac
      721 tctgaatagc ttggaataag ccctgatttt tcaagttggt tgtgtagaaa ccaggatcga
      781 aggcaaagct tctaagactg aagacccaga tcgtggccag caacaacacc acaaagatca
      841 ggccgaaggg cagtgcgatt atttttctca tggacgattt ctcttcagaa tttgaacggc
      901 aatacgacga cctgcgcagc aatcgatcga tcgcctgcgc gcgcttgcac ggcttgatcg
      961 tcaattgcca gcgcagcctt gatgtaaccg agtgccagcc agccccgatc gggcgaatga
     1021 gcgatgctgg tgatagatcc gacgtcacca tcatccgcga aaatgcgagc gggcaagttg
     1081 atttcagatt ccaaccgcag gccgatcaat tgtttggcca aacgctggcg gctttccatc
     1141 cgtgcaatga tctcctgtcc ggtgtaacag cctttagtga aactcacgtc cggccataga
     1201 ttggcctcaa gcggaatgat ctcgtcggtc aactcacgtc catattccgg ctggcctgct
     1261 tcgacacgca agacttgata agtttcctcg gcaatcggcg tcgcgttggc atcgatcaat
     1321 ttcgatcgca agcccgacaa cttatcagcc tgaacgataa gatgaaagcc attagtcccg
     1381 agtgaatccg cgcgagcgat cagcacttct acctcgccga ggttggcact ccgccaatga
     1441 tgcaagccta atgatgatac atcgatgcca acaactcgct ggaacgtttc gggcgcacgc
     1501 ggcccgtaaa ccgaaagcat cccaagttct tgagtcgcgt ctacaatctg cacatcgtca
     1561 ttgaagaaaa tgtatttctt caaccacgac atcacgggcg cttgggcgtc acgcgatgtc
     1621 agcatgatca aatcattctc gcggacatag accatcgtgc gatcaatgat gcgcgcgatc
     1681 ggcgtggcga agatcgtcgc agccccctgc ccgatctgaa gtttcagcag atcgttcgtt
     1741 gacatgcggt gcagaaagtc cagccgcgtg ctgccgctga tccgcaatcg cccgcgcgat
     1801 gaagaatcga tcaatacgca gccattcttg gcagcggcat attcagtttc gatagttggt
     1861 gcaagtttag ttggcattta ttaaccagcc gtcagtgcga gccgccgatc ccgcaaacaa
     1921 cgggcgggac ctt
//
LOCUS       DASRTJ010000733         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_149215,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000733 DASRTJ010000000
VERSION     DASRTJ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149215"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFF70_14820"
     CDS             <1..696
                     /locus_tag="VFF70_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46009.1"
                     /translation="ITAARYTVTIFLRGITTSDAKFSDFYFDTASLVYSFPISVTTRQ
                     ALPWPLTSTVAITLQAPISLTQLNASLADPNGLSIPLMYLGTTGVTPTVFSRWNFSPA
                     IAGAHHFTLTAAQLPHPFIQPIIVSAMQYNTIQDHLPPPSPITFTLYSPISLTGITSV
                     LTDPLGSLTTFNYIGSNYISPTYAFQWTFTSTIVGVYTLGLFANQFTHHWLIPILAAS
                     SRAYLPVVMRAAP"
     gene            878..1090
                     /locus_tag="VFF70_14825"
     CDS             878..1090
                     /locus_tag="VFF70_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46010.1"
                     /translation="MNNTYLQIFVFSLLLLYIVNLRVKLWLDHKVIQQFQKSAIIVPN
                     PDKKHDFGPVALMIAALALLLAMAHL"
     gene            complement(1116..1448)
                     /locus_tag="VFF70_14830"
     CDS             complement(1116..1448)
                     /locus_tag="VFF70_14830"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZY46011.1"
                     /translation="MTFTLEQIMQHMPEAFVPEKAAGLAANVQLDFGESGSYAVRIAA
                     DRCTVTPGPIDQPDATLIASAETYIAMIEGRLETMKAFMSGQLKVKGNLNLLLKFQSL
                     FDPSRVQR"
     gene            complement(1456..2487)
                     /locus_tag="VFF70_14835"
     CDS             complement(1456..2487)
                     /locus_tag="VFF70_14835"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZY46012.1"
                     /translation="MKIVVIGAGAIGGYLGGKLSDRHEVVLVGRTLLVATINSRGLKI
                     IEPRLERWARARAVDSIETAFQTIDRFDLALFTVKNFDTETTIEQMKPFADRIDRILS
                     FQNGVESEEVLGRVFGRNKIIAGTILNPISIPEIGSVRLEKYKGGLGLASIDGQSIEA
                     LTVTLKDTGLLVRVYTDYRSMKWSKLLLNLIGNATSAILDMTTRETFNDRMVFRVEVA
                     ALREAVQVMRALSIRPITLPGYPVPLLVFALRFLPIPLLQIIMRPLAAAGRGDKPPSL
                     LMDLRNGKTKSEIDDLNGAIVRWGESIGVKTPINEFLTNIVKDLAAGRVDRAVWRRQT
                     TELAQTLFP"
     gene            complement(2489..3013)
                     /locus_tag="VFF70_14840"
     CDS             complement(2489..3013)
                     /locus_tag="VFF70_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZY46013.1"
                     /translation="MRLQGKRVAFLLDEGFEDLEFWVTVMRLREEGATVTILSKDAKT
                     EYRGKNALTALPDASFKDAKSTDFDAMVVPGGWAADKLRRYTEVKRLVKEIYDQKKIV
                     GLICHAGLVGISAGIVKGHKSTGSLGIKDDLINAGATFVDEPAFRSGNIVWGRVVADI
                     PDFCRELVAAIENP"
BASE COUNT          773 a          947 c          751 g          871 t
ORIGIN      
        1 atcacggctg ctcgttacac ggtcacgatc tttttgcgtg gcattactac cagtgacgcg
       61 aagttcagcg acttttactt cgatacagcc agcctggttt atagttttcc gatcagcgtt
      121 accacgcgac aggctctccc ctggccgctg acctcaaccg tcgccatcac gttgcaagcg
      181 cctatatctt tgacgcagct caacgcgagc ctcgctgatc cgaacggcct gtcgatccct
      241 ttgatgtatc tcggcacaac cggcgtcaca cccaccgttt tttctcgctg gaattttagt
      301 ccggcgatcg ccggagcgca ccacttcaca ctaaccgccg cccaattacc acatccgttt
      361 attcagccga tcattgtgtc ggcgatgcag tacaacacaa ttcaagatca cttgccgcca
      421 ccgtcgccca ttaccttcac gctctattca cccatttcac taacgggtat cacttcagtg
      481 ttgactgatc cgctcggttc attaacgaca tttaactaca tcggatcaaa ttacatcagc
      541 ccgacgtatg cattccaatg gacgtttacc tcgacgatcg taggtgtgta tacgctaggt
      601 ctctttgcaa atcaattcac ccaccattgg ctgatcccaa ttctggccgc ctcgagtcgc
      661 gcgtacttgc ccgtggtcat gcgcgcagcg ccatagtccc agccttcact gtgccgccct
      721 gaaaataatt gctacttgac aacctggcta gccggagtat acttagtgta taatatacac
      781 ttcggctgag ccgaatttat ttttgcgatt aattagtgtt agattaacac tattagaaat
      841 gcctgaaaaa acttggggca aacaagcgag gaagatcatg aataatacat atctgcaaat
      901 ttttgttttc agtctgttgt tactatatat cgttaactta cgcgtgaagc tgtggctcga
      961 tcacaaggtc atccagcaat ttcagaaatc ggcgatcatc gtgccgaacc cggacaagaa
     1021 acatgatttc ggcccggttg cacttatgat cgcagcgctg gcgctcttgt tggccatggc
     1081 gcacctgtaa gcgttcgctg agcctggcgg ttcaatcatc tctgcactcg cgacggatcg
     1141 aacagtgact gaaacttcag cagcaaattt aaattgccct tgacttttaa ctgccccgac
     1201 ataaaagctt tcatcgtctc gagccgtccc tcgatcatgg cgatgtaggt ctcggcgctg
     1261 gcgatcagcg tcgcatccgg ttgatcgatc gggccgggcg tcaccgtgca tcgatcagca
     1321 gcaatgcgca ctgcgtagct tccactctcg ccaaaatcca actgcacatt ggcggcgagc
     1381 cctgccgctt tttcaggcac gaaagcttct ggcatatgct gcatgatttg ttcgagggtg
     1441 aatgtcatga tcccctcacg ggaaaagagt ctgcgctaac tcagtcgttt gtctacgcca
     1501 caccgctcgg tcgacgcgac ccgcggccaa atcttttacg atgttggtca agaattcatt
     1561 gatgggtgtc tttactccga tcgattctcc ccaccgaacg atcgcgccgt tgagatcgtc
     1621 aatctccgat ttagttttgc cgttccgtaa atccattaac aacgacggcg gcttatcgcc
     1681 tcgacccgct gcggcgagtg gccgcataat gatttgcaat aaggggatcg gcaggaatct
     1741 caaggcaaag acaagtaatg gcactggata accgggcagg gtgatcggtc tgatcgaaag
     1801 cgctcgcatc acctgcaccg cttcacgcaa ggctgcgact tcgacgcgaa agaccattcg
     1861 atcattaaac gtctcgcggg tcgtcatgtc gagaattgcc gatgtcgcgt tgccaatcaa
     1921 attcagcagc aacttgctcc acttcattga tcggtagtcg gtgtagacac ggactagcag
     1981 cccggtgtct ttcagcgtca cggtgagcgc ttcaatggat tgtccgtcga tcgatgccag
     2041 tcccaacccg cctttgtatt tctccaaacg gactgatccg atctctggaa tcgaaatcgg
     2101 attgaggatg gttccagcga taatcttatt gcgtccgaag acgcggccta gcacttcctc
     2161 gctctcgacg ccattttgga atgagagaat gcgatcgatt cgatcagcaa atggtttcat
     2221 ttgctcgatc gtcgtctcgg tgtcaaagtt cttaaccgta aacaaggcca agtcaaaacg
     2281 atcgatcgtt tgaaaagccg tctcgatcga atccacggcg cgcgctcgtg cccagcgttc
     2341 taatctcggc tcgatgatct tcaaccctct tgagttgatg gttgctacca acagcgtgcg
     2401 cccgaccagc accacctcat gacgatcaga caatttaccg ccaagatatc cgccaatcgc
     2461 gcctgcgcca atgacgacga ttttcaattt acggattctc aatcgccgca acgagttctc
     2521 ggcagaaatc aggaatgtcg gcgacgacgc gtccccacac aatgttgccg ctgcggaaag
     2581 ccggctcatc gacgaaggtc gcgcccgcgt tgatgagatc gtctttgatg cctagactgc
     2641 ccgtcgactt atgacccttg acaatgcccg ccgaaattcc aaccagaccc gcatgacaga
     2701 ttaagccgac gatctttttc tgatcataga tttctttgac taaccttttc acttcagtat
     2761 agcggcgcag tttatcagcc gcccagccgc cgggcacaac catcgcgtcg aagtcagtcg
     2821 atttggcgtc tttgaaactc gcatcaggta atgcggtcaa tgcgttcttt ccgcgatatt
     2881 ctgtctttgc atctttgctc aaaatagtga cggtcgcccc ttcttcgcgt aagcgcatga
     2941 ccgttaccca gaactccaaa tcttcaaagc cttcgtcaag tagaaacgca actcgttttc
     3001 cttgtaatct catcttacgc ctccgaatgc ataatgttta atgcggaatg taaaattagg
     3061 aattctgcat tgtgaattgt gaatttatta ggctgggttg tccgcgtgca aagtcttccg
     3121 ccctcattgc tttacgccca gcgaattgaa tctcgcgtaa aacaatcatg cccgatccgg
     3181 ttattactcc tattgatcca tcttccagtt taatcacatg ccccaacttg gagcttgaaa
     3241 cttgcgactt gctttttatt tcaaccttca acaccttcag tatcgatcta ctccagaagg
     3301 tgtacgtgcc tggccaaggt atatacgccc gtaccattcg at
//
LOCUS       DASRTJ010000734         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_377073,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000734 DASRTJ010000000
VERSION     DASRTJ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377073"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFF70_14845"
     CDS             <1..1160
                     /locus_tag="VFF70_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZY46014.1"
                     /translation="LRTDRQALQRLREAAEKAKIELSSLMETEISLPFITADASGPKH
                     LSIKLTRSKFEQLSVNLVKRLRTPFEQALNDAKIMPDKIDEVVLVGGATRMPMVQSLV
                     RELTGGKEPNKSVNPDEVVAIGAAIQAGVLGGEVKDVLLLDVTPLSLGLETLGSVMTT
                     LIPRNTTIPYKKTEVFSTAEDAQTAVDIHVLQGERPIAADNMTLGRFRLEGIPTAPRG
                     MPQIEVTFDIDANGILNVSAKDKATNKEQKIAITASTNLSKADVDKMVQDAQRNNAED
                     NRRREVIQARNEADTVIYQAEKSLRDLGDKVGAPDRQKVEAKIIDLKSAMKSDNVSQI
                     RSTINDLQQASMAIGQNMYQSDASGGSTGPRSEQPGPSDEEGVVEGEYRQV"
     gene            1307..>1800
                     /locus_tag="VFF70_14850"
     CDS             1307..>1800
                     /locus_tag="VFF70_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZY46015.1"
                     /translation="MFFFDPMYLVFALPALLFAFYAQMKVQGAYKKWLQVANTRGMTG
                     QEAARYLLSANQLGDIGIEGIPGKLTDNYDPRDKTLHLSQEVANGRSVAAIAIVAHEV
                     GHAVQDAQGYGPMKVRGAIVPAITVGSWVGPILFFIGFLARSMPLAWLGVILFAGTAL
                     FALIT"
BASE COUNT          444 a          466 c          522 g          368 t
ORIGIN      
        1 atctgcggac cgatcggcag gctttacagc gattgcgcga agcggccgaa aaggccaaga
       61 tcgaactatc gagtttgatg gagaccgaga tcagtttgcc attcatcacc gcagatgcat
      121 ccggcccgaa gcatctatcg atcaagctga ctcgatcgaa gttcgagcaa ctgagtgtga
      181 acctggtcaa gcggctgcgc acaccattcg aacaagcctt gaatgatgca aagatcatgc
      241 cggacaagat cgacgaagtg gtgctggtcg gtggcgcaac gcgcatgccg atggtgcaat
      301 cacttgtacg cgaattgacc ggcggcaagg agcccaacaa gagtgtgaat cccgatgaag
      361 tggtcgcgat cggcgcggcg attcaagcgg gtgtgttggg cggcgaagtg aaagatgtgt
      421 tgctgctcga tgtcacaccg ctgtcgctgg gtcttgagac acttggcagc gtgatgacga
      481 cgctcatccc gcgcaacacg acgattccgt ataagaagac tgaagtcttc agcacggccg
      541 aagatgctca gacagcggtt gacatccatg tgttgcaagg cgagcggccg atcgccgcgg
      601 ataacatgac gctcggtcga ttccgcttgg agggtattcc aactgcgccg cgcggcatgc
      661 cgcaaatcga agtcacattt gacatcgatg ccaacggcat cttgaatgtg tccgcgaaag
      721 acaaagcgac gaacaaggaa cagaagatcg ccatcaccgc ctcgacgaac ttgtcgaagg
      781 ccgatgtcga taagatggtg caggacgcac agcgcaacaa cgcggaagac aatcgacgcc
      841 gcgaagtgat tcaagcgcgc aatgaagccg acaccgtgat ctatcaggcg gaaaagagtc
      901 tgcgcgatct gggcgataaa gtcggtgcgc cggatcgaca aaaggttgaa gcgaagatta
      961 ttgatcttaa atcggcgatg aagagcgata acgtcagtca aattcgatcg acgatcaatg
     1021 atctgcagca agccagcatg gcgatcggtc aaaacatgta tcaatccgat gcttccggcg
     1081 gctctaccgg tccccgatcg gaacagcctg gcccgtcgga cgaggaaggc gtagttgaag
     1141 gcgaatatcg acaggtctaa agaaactctt tgaccgcgcg ggtctttgaa acccgcgcgg
     1201 tcttctatcc aaccctcgta aggtttcata gatccgcatg atataatctg aagagttgaa
     1261 tgacctcgca ggttttcaaa aaccatcagg tctcacggag gccaaaatgt ttttcttcga
     1321 tccgatgtac ttagtctttg cgttacccgc tctgctgttt gcgttttatg cgcagatgaa
     1381 agtgcagggc gcgtacaaaa aatggctgca agtcgccaac acacgcggta tgaccggcca
     1441 agaggcagcg cgctatttgt taagcgccaa tcaactgggc gatattggca ttgaaggcat
     1501 tcccggcaaa cttacagata actacgatcc gcgtgataaa actctgcact tgagccagga
     1561 agttgcgaat ggccgatcgg tagccgcgat cgcgatcgtg gcacatgaag ttggtcacgc
     1621 cgtacaggat gcgcagggct acggcccgat gaaggtgcgc ggcgcgatcg tgcccgccat
     1681 caccgtcggc tcgtgggtcg ggccgatctt gttcttcatc ggcttcttgg ctcgatcgat
     1741 gccgctggcc tggctcggcg tgatattgtt cgcaggcact gccttgttcg cgttgatcac
//
LOCUS       DASRTJ010000735         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_302248,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000735 DASRTJ010000000
VERSION     DASRTJ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302248"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFF70_14855"
     CDS             complement(<1..352)
                     /locus_tag="VFF70_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZY46016.1"
                     /translation="MNDKRQFVDTNILVYAHDSSAGAKHIRARALLAELWRSNTGCLS
                     IQVLQEFYVNITRKVARPLDQDTARQIIVDLGRWTVHSPTVNEVVEAIALQERHTLSF
                     WDAMIVTSAIHLGCH"
     gene            complement(342..584)
                     /locus_tag="VFF70_14860"
     CDS             complement(342..584)
                     /locus_tag="VFF70_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZY46017.1"
                     /translation="METQNITLALPRKILRQVKTIAAQRHTSVSRLLTEQLEAMLADE
                     QGYTRAQTRHLALLEHAPDLGTRGRLRIKREALHER"
     gene            976..2361
                     /gene="mgtE"
                     /locus_tag="VFF70_14865"
     CDS             976..2361
                     /gene="mgtE"
                     /locus_tag="VFF70_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZY46018.1"
                     /translation="MAKNNSHTKVVTYEEVEQQVRTALAIDDQVQLKAAVAHEHPSNL
                     AAVIDRLNHQQRHQVFALLTPAQAPKVLDELSIEATRQLLDDLSAQEIGALLDRMPMD
                     EAAEILTEDYPQREQELLAAMSPARGEQVQALLRYPKHSAGRLMTHKFAQVRPDLSAA
                     EALTYLREINADVETLTNLYVLNDEAQLIGVVSLREVVTAPPERTLAELMNTDLVTVT
                     PETDREEVAHLVSHYDYLALPVVTQDKHMLGIIPVDRVIDVLVSESTEDILRFGGMEG
                     GGTIDQPYFTVALPLVIRKRVGWLLLLFLAETLTGNVLRIFEGELSKVVALSFFIPLL
                     IGTGGNTGAQTVSTLIRGLALGEIQMRDIWRVLRRELLSGFLLGILLGTVGFVRTLLW
                     GNGAPIALIVGLTILAICTWANTVGALIPMVAHKLKIDPAVVSAPLITTLVDATGLAI
                     YMLIAKLILGL"
BASE COUNT          546 a          626 c          693 g          581 t
ORIGIN      
        1 catggcagcc caagtgaatt gcgctggtaa cgatcatagc atcccagaac gacaaagtgt
       61 gtcgctcctg aagtgcgatc gcttctacaa cctcattaac ggtcggcgaa tgaaccgtcc
      121 agcgccctaa atcgacaatg atctgtcgag ccgtatcctg atcaagcggt cgggctactt
      181 tccgtgtgat gttcacgtag aattcttgca acacttgaat gctgaggcaa ccggtattgc
      241 tacgccataa ttcggcgagc agcgcgcggg cgcgaatatg tttagcacca gctgaactat
      301 cgtgagcata gaccaatatg tttgtatcaa cgaattggcg cttatcgttc atgcagcgcc
      361 tcgcgcttga tccgtagtcg gccacgcgta ccaagatcag gcgcatgctc cagcaacgcc
      421 agatgacgtg tttgcgcacg agtatagcct tgctcatcgg ccagcattgc ttccaactgc
      481 tcggtcaaca gacgcgagac ggacgtatgg cgttgagcgg cgatagtttt gacttggcgc
      541 agaattttgc ggggcaatgc taaagtaata ttttgagtct ccatggcaac ctccatactg
      601 agtatatcac gttcacgttt ttccgtgaag agtcgcttga ttcagttctt tgataagtat
      661 gtgaaaggca gttgatcagg gatcggggat cagagcattg actatgtcat gcccgaagac
      721 atgcggtgca gtgctcccgt gtttttatcg ggcatccaga ttgatggtta gcaagtcacg
      781 ttaatagaat gactggattc ccgctttcgc gggaatgacg cctccgatta cattctttga
      841 taagtatgtg aagaacatcg gagcgcaagg atgagcgctt aacttttcag gaattgacag
      901 tcgggcgtgc agctactata gttggcctcg aagttcgctt gatcagctta atcagtcagc
      961 cagaggagga agtctatggc caagaacaac agccatacca aagtagtgac gtatgaagag
     1021 gttgagcagc aggtccgcac tgctctagcg atcgatgatc aagtccagtt aaaggcggcg
     1081 gtggcacatg aacacccgtc taatctagcc gccgtgatcg atcgcttgaa ccatcaacag
     1141 cgacatcaag tcttcgcgct gttgaccccg gcacaggcgc ccaaggtact ggacgaactt
     1201 agcatcgaag cgacgcgcca gctgctcgac gatctatcgg cgcaagaaat cggcgcgctg
     1261 ctcgatcgca tgccgatgga tgaagctgcc gagatcttaa cggaagatta tccacaacga
     1321 gagcaggaat tgctggctgc gatgtctcca gcgcgcggcg aacaggtgca ggcgctattg
     1381 cgttatccga agcacagcgc cgggcggctg atgacccaca aatttgcgca ggtaaggccc
     1441 gatctatccg cggcagaagc gttgacctac ttgcgggaaa tcaacgcgga tgtggaaacg
     1501 ctgacgaacc tgtacgtgtt gaatgatgag gcgcagctga tcggcgtcgt ctcgctgcgc
     1561 gaggtggtga ctgcgccgcc cgaacgcaca ttggctgaat tgatgaacac cgatctggtc
     1621 acggtcacac cggaaaccga tcgcgaagaa gtggcgcatc tggtctcgca ctacgattat
     1681 ctggcgctgc ccgtcgtcac tcaggataaa catatgctcg gcatcattcc agtcgatcgc
     1741 gtgatcgatg tgctggtctc cgagagcacg gaagacatct tgcgcttcgg cggtatggaa
     1801 ggcggcggaa cgatcgatca gccgtacttt acggtcgcat tgccgttggt cattcgcaag
     1861 cgagtgggct ggctcttgct gttgtttctg gctgagaccc tgaccggcaa tgtcctgcgg
     1921 atcttcgagg gcgagttgtc gaaggtggtg gcactctctt tctttattcc actgctgatc
     1981 ggcacgggcg gcaacactgg cgcgcaaact gtttcgactc tcatccgggg tttggctctc
     2041 ggcgaaattc aaatgcgcga tatctggcgc gtgcttcgac gtgagttgtt gagcggtttt
     2101 ctgcttggca tcctcttagg cacagtcggc tttgtgcgaa cgttgttgtg ggggaatggc
     2161 gccccgattg ctctaatcgt ggggctgacc attttagcca tctgcacctg ggcgaatacg
     2221 gtgggcgcgt tgatcccgat ggtggctcat aagctgaaga tcgatcccgc cgttgtctcg
     2281 gctccactga ttacgacact ggtcgatgcc acgggcttgg ctatttatat gttgatcgcc
     2341 aaactcattc tggggctgtg aggaacagct agaacaatca aagcccgatc tgggttagcc
     2401 agatcgggct tccatcctcc gtcctcggtc gatcaattta gcttaa
//
LOCUS       DASRTJ010000736         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_177738,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000736 DASRTJ010000000
VERSION     DASRTJ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177738"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..359)
                     /locus_tag="VFF70_14870"
     CDS             complement(225..359)
                     /locus_tag="VFF70_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46019.1"
                     /translation="MNMWMGLLLFIVLFASKKFEDRSGHFDIRSLLLLLSLIYMALKG
                     "
     gene            529..1269
                     /locus_tag="VFF70_14875"
     CDS             529..1269
                     /locus_tag="VFF70_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZY46020.1"
                     /translation="MIRLLLVEDNQSLRAAMRSGLEAAGQVSVIAETDRGEESIVLAL
                     ADPPDAILMDVRLAGELNGVQAAITIRREIARLPIVFYSIQDDERFYRDFLASGILTH
                     YAYVRKSNYLLPQMVVPLLRDAMAGRSFIDPEIEQRVQEVRLKDEDSPLSLLEPNESE
                     VVRLLAQGMTNEQIASRLGFHDRRAVSRTNGQIYSAWGLDNSNEDEKTARARASLIYN
                     TGRMIVWNESGTPCVRNAKGEWIPMYDE"
     gene            1511..3757
                     /locus_tag="VFF70_14880"
     CDS             1511..3757
                     /locus_tag="VFF70_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY46021.1"
                     /translation="MFDYALFAATLAISLFDTVTLLWLGLTVFLTGNRRTSATIAGSA
                     GLILGSIFFGGHTLIITHTLGSGSFGVNIVWRVMWIVAVAAPFFWGLAIFYYAGDPNT
                     GRWVRRILTAAGLVLIALLIINPIPSFNDLLFASALTPSIAWAYVPYLFLCFTLPLIA
                     LRRRRSTPPSMGGTRGADPFRRARPWLIAAGSMLALAVIAFAFTAYSIVPRAIPITHL
                     TLDINRAVYVADGIIAGFIGLAIIFLGRAVLSNNVLTERPQSGQGFFARWRSVVIVSI
                     VGSLGVAFLYFAPIRPIYSLLMTALLGMTAYALFNWRQYAEHEAFMKRLRPFVTSLHL
                     YDHLISAHEDEWHESRELFDALCRDALDARQAILLYTPADEVEGSRRGEQRIEYHWPI
                     DHDELLSTALNSIEWIHLDADHWALPLADSRGATHAVPVRYSPQGVPKHTVRTVGRLI
                     LGPKINGSEYTAQELQVAATCAERILDALAGEQLARVAVSLLRQRIAQVQVLSGQHKR
                     WLHDEVLPQIHLALLKIEALRNIEHDAGYQADKARVLPYINDATNALTQSHRRLAALV
                     RDMSAAAPTGLESDGLIAALKSALDHDFRDSFDRVEWQVSDSGNDRSQKLPLFASEVI
                     FYAAQEAIRNAARHARGDDTNRKLNLFVSIESDSDLKIVVADDGVGMKMHPERSAESE
                     GVSAQSTPDGPPTLPRRIGNGQDAVASNESGSGLLFHRTMLTIIGGTLSIEDRSGGGT
                     QVAIEIPT"
     gene            3835..>5254
                     /locus_tag="VFF70_14885"
     CDS             3835..>5254
                     /locus_tag="VFF70_14885"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZY46022.1"
                     /translation="MAITSTRPTDLRQRRGRITRFFFRAIVSAIFWDVLVRNLGGRRW
                     VARTALNRYVRLARRFRELAIELGGVMIKLGQFISSRVDILPKEIIDELASLQDEVPP
                     EAFDRIQSLIESELGRSIDEVFTSIDRTPIAAASLGQAHRVQLKPDQSAIVKVQRPGI
                     ESIVAVDLEALRSVMGWLKRYGPIRRRADLDQLLDEFRRTLYEELDYLAEGRNAERFA
                     ADFKDWRDIRIPQIHWELTTRRVLTMQDIGAIKIGDVTGYTSRGVTAHDVAHTVFRFY
                     MQQIFVNGFFHADPHSGNFFVEPKKVDGLTKFTLNVVDFGMVGSISSQLKATLRDGFI
                     GIATRDVHRVVHSMDASGWLLPSADRRAIERAADKVFARFWGISMGDLKNLDLTEMRG
                     FAGEFRSLLIQFPFQIPANLLFLGRALGILSGLATQIDPHFNVFASAVPFAEKMIADE
                     SGSIFRTVIDQSIEIGKSIVRLP"
BASE COUNT         1117 a         1392 c         1488 g         1257 t
ORIGIN      
        1 agcgtatcag ccagcacacc taagatcgcg gcgacgatga taacggtcag tcctgccttc
       61 gcatttgagt tcatagattc ctcattatgt cgtcattgcg agggcgaagc ccgaagcaat
      121 ctccttgaga gtagatcaat cgatcgatcg ggagattgcc acgtcgcttc acctgcactg
      181 caacgcatgc agtgcggtgc aagtgtcagc ggctcgcaat gacgttaacc tttcaacgcc
      241 atatagatca gcgacagcaa taggagcaga ctgcgaatat cgaagtgtcc tgatcgatct
      301 tcgaatttct tggatgcaaa caatacgatg aacagcaaca atcccatcca catgttcatg
      361 gcttgcctcc tgtctcgata ccatcctagc cgaagtccgc ctgagatcga aggcttcgcg
      421 tgtagcaatc gtcgcacttg agtgcttgac tgtaatcaca gatgactcga taatcgagat
      481 cgccactata ggtatacgcc tgatattttc aacgtaggct aagcgtgcat gattcgttta
      541 cttcttgtcg aagacaatca atcgttgaga gccgccatgc gatcggggct ggaggcggcc
      601 ggccaagtgt cggttatcgc tgaaactgat cgtggcgaag aatcgatcgt gctggcgttg
      661 gcggatccgc cggatgcgat tttgatggac gtgcggttag ccggcgaact gaatggcgtg
      721 caagctgcga tcacgatccg acgtgagatc gcgcgtctgc cgatcgtgtt ttattcgatt
      781 caagatgatg agcggttcta tcgcgacttt ctggcgtcgg gcatcctcac gcattacgcg
      841 tatgtgcgca aaagcaatta cttgctgccg caaatggtgg tgccgctgct gcgcgatgcg
      901 atggcgggcc gcagcttcat cgatccggag atcgaacagc gcgtgcaaga agtgcgctta
      961 aaagatgaag actcgccgct gagtttgttg gagccgaacg agagcgaggt cgtgcgattg
     1021 ttggcgcagg gcatgacgaa cgaacagatc gcgtcgcggc tgggcttcca cgatcggcgc
     1081 gcagtcagtc ggacgaatgg gcaaatctat tcggcgtggg gactcgataa ttcaaacgaa
     1141 gatgagaaga cggcgcgggc gcgggcttca ttgatttaca acacgggtcg tatgatcgtg
     1201 tggaatgaaa gtggtacgcc atgcgtgaga aatgcaaaag gtgagtggat tccaatgtat
     1261 gacgagtgac gagcgatgcg tgacgagtaa tgagtaacaa gtaatgagta caagaaattt
     1321 aaccatcatt gcgagcgaag tccttagatg gcacagcctt cttaggatgt gccacgaagc
     1381 aatctcagtt gattatcgca gagattgctt cgtcgtgcct tcggaacttc tcgcgatgac
     1441 gatattaact agaagcgatc cgccatcacc ttgtccattg aaaattgtta attgataatt
     1501 gatcattgcc atgtttgact acgctctctt cgccgccacg ctcgccatct cactcttcga
     1561 caccgtcaca ctcttgtggc ttggtctgac cgtgttcctc accggcaatc gacgcacgtc
     1621 cgcgacgatc gccggcagcg ccggcctgat tctcggatcg atcttcttcg gcgggcacac
     1681 cctgatcatc acgcacacgc tcggttcggg aagtttcggc gtcaacattg tgtggcgtgt
     1741 gatgtggatc gtcgccgtcg ccgcaccgtt cttttggggc ctagcgatct tttattacgc
     1801 gggtgatccc aataccggtc gttgggtgcg gcgcattctc actgccgctg gcctcgtgtt
     1861 gatcgcgctc ctcatcatca atcctattcc ttcatttaat gatttgctct ttgcgtcagc
     1921 cttgacgcca tccattgcgt gggcttatgt gccgtatctc ttcttatgct tcacattgcc
     1981 gttgattgca ctgcgtcgac ggcgctccac ccctccatct atggggggaa cgaggggggc
     2041 cgatccgttc cgccgcgcgc ggccatggtt gatcgctgcc ggctcgatgc tggcgctggc
     2101 ggtgatcgct ttcgcgttta cggcttactc gatcgtacct cgtgcgatcc cgatcactca
     2161 tctcacgctc gatattaatc gcgctgtcta cgtggccgat ggaattatcg ccggattcat
     2221 tggcttagcg atcatctttt tgggccgcgc cgtcctatcg aacaacgttc tgaccgaaag
     2281 accacaatct ggccaaggat ttttcgcgcg ctggcgcagc gtagtgatcg tatcgatcgt
     2341 cggatctttg ggtgtggcgt tcttgtattt cgcgccgatt cgaccgatct acagtttgtt
     2401 gatgaccgcc ttgctcggca tgacggcgta tgcgttgttt aattggcggc aatacgcgga
     2461 acatgaagcg ttcatgaaac ggttgcggcc ttttgtgacc agtctgcatc tgtacgatca
     2521 tttaatctca gcgcacgaag atgaatggca tgagtcgcgt gaattattcg atgcgctgtg
     2581 ccgggatgcc ttagacgcgc ggcaggcgat actgctgtac actccagccg acgaagtcga
     2641 ggggagcagg cggggagaac agcgaattga atatcactgg ccgatcgatc acgatgaatt
     2701 gttgtccact gcattgaact cgatcgagtg gattcatctc gatgcagatc actgggcttt
     2761 gccgttagcc gattcacgcg gtgcgacaca tgcggtaccc gtgcggtata gcccgcaggg
     2821 cgtacccaag cacacagtgc gtaccgtcgg ccgcttgatc ctcggtccga agatcaacgg
     2881 gagtgaatac accgcgcaag aactgcaagt ggccgcgacg tgtgccgaaa gaattctcga
     2941 tgcgttagcg ggcgaacagc tagcccgagt cgccgtcagt ttgttgcggc agcgcatcgc
     3001 acaagtgcaa gtgctgagcg gccagcacaa gcgttggctg catgacgaag tcctaccgca
     3061 aattcatttg gccttgctca agatcgaagc gctgcgcaac atcgaacacg atgccggtta
     3121 tcaagcggat aaagcccgcg tgctgcccta tatcaacgac gcgacgaacg cgttgacgca
     3181 atcgcatcga cgcttagcgg ccttggtgcg cgatatgagt gccgccgcac ccactggact
     3241 cgaaagtgat ggcttgatcg ctgcgttaaa gagcgcgctc gatcatgact tccgcgacag
     3301 tttcgatcgc gtcgaatggc aagtgagtga ctcggggaac gatcgatccc aaaagttgcc
     3361 gctgtttgcc agcgaagtaa tcttctatgc agcacaggaa gcgatccgca acgcagctcg
     3421 gcatgcgcgc ggcgacgaca caaaccgtaa attgaatcta ttcgtttcga tcgagagcga
     3481 ttctgacttg aagatcgttg tcgcagatga tggtgtggga atgaagatgc atcctgagcg
     3541 gagcgctgaa tccgaaggag tcagtgcgca gtcgacgcct gacggtccgc caactctccc
     3601 gcggagaatt gggaatgggc aggacgcggt tgcatcaaac gaatccggca gtgggttgct
     3661 attccatcgc acaatgctga cgattattgg tggcacactc tcgatcgaag atcgatcggg
     3721 cgggggaacg caagtggcga ttgaaattcc aacgtagggg caggttttaa acctgcccct
     3781 acaatttaag atgctgtata cttacccctg aatatttcct ccgaaaaaat tctcatggct
     3841 ataacttcca ctcgccccac cgatttacgt caacggcggg ggcgcatcac tcgtttcttc
     3901 ttccgcgcga tcgtgtctgc gatcttttgg gatgtcctcg tgcgcaatct gggcggacga
     3961 cgctgggtcg cgcgcaccgc actcaatcgc tacgttcggc ttgcccgccg cttccgcgaa
     4021 ttggcaatcg aactcggcgg cgtgatgatc aaactcggcc agttcatcag cagccgtgtc
     4081 gatattctgc ctaaagaaat tatcgacgaa cttgcctcgc tccaggatga agttccgcct
     4141 gaagcatttg atcgcattca atcgttaatc gaatctgaat tgggtcgatc gatcgatgaa
     4201 gtgtttacct cgatcgatcg cacgccgatc gcggccgcgt cgctgggcca agcgcatcgg
     4261 gtgcagctca agcccgatca atcggccatc gtcaaagtgc agcggcccgg catcgaatcg
     4321 atcgtcgcag tcgatctcga agcgctgcga tcggtcatgg gctggctgaa acgctacggc
     4381 cccattcgcc gccgcgccga tcttgatcaa ttgcttgatg aatttcgccg caccttatat
     4441 gaagagttgg attacttagc cgaaggccgc aatgccgagc gcttcgccgc cgatttcaaa
     4501 gactggcgcg atatccgcat tccacagatt cattgggagt tgacgacgcg gcgcgtgctg
     4561 acgatgcagg acatcggcgc gatcaagatc ggcgatgtga ccggctatac atcgcggggc
     4621 gtaacggcgc atgatgtggc acacaccgtc ttccgatttt atatgcagca aatattcgtc
     4681 aacggtttct ttcacgccga tccccattcg ggaaatttct ttgtcgaacc gaagaaagtg
     4741 gacggcctaa cgaagtttac tttgaatgtg gttgactttg gcatggtcgg ctcgatttcg
     4801 tcgcagttga aggccacgct gcgggatggc ttcatcggca ttgcgacgcg cgatgtccat
     4861 cgcgtcgtgc actcgatgga tgcgtccggt tggttattgc cgtccgccga tcggcgcgcg
     4921 atcgaacgcg cggccgataa agtgtttgcg cgcttttggg gcatcagtat gggcgatctt
     4981 aagaatctag atctcaccga gatgcgcggt tttgcgggcg aattccgatc actgttgatt
     5041 cagtttccgt ttcagattcc ggcgaatctg ttgtttctcg gccgcgcgtt ggggattcta
     5101 tcggggctgg cgacgcagat cgatccgcac ttcaatgtgt ttgcttcggc cgtgccgttt
     5161 gccgagaaga tgatcgccga cgaaagcggt tcgatcttcc ggacggtgat tgatcaatcg
     5221 atcgagatcg gtaaatcgat cgtgcggctg cccg
//
LOCUS       DASRTJ010000737         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_150162,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000737 DASRTJ010000000
VERSION     DASRTJ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150162"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..471)
                     /locus_tag="VFF70_14890"
     CDS             complement(247..471)
                     /locus_tag="VFF70_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HZY46023.1"
                     /translation="MPRLRVLSGREACKIMSKQGFVEIRQRGSHIIMQRQAENTTTTV
                     PVPDHDELRIGTLLGIIRQSGLPRSLFETK"
     gene            complement(464..730)
                     /locus_tag="VFF70_14895"
     CDS             complement(464..730)
                     /locus_tag="VFF70_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HZY46024.1"
                     /translation="MEKVLQLTAVIEREGDGYVATCTELDVVSQGDTVEQARLNLLEA
                     VEGFFEVASPSEIRRRLKKETYIMSIMPAAPNIKLKQVARLRHA"
     gene            complement(827..1612)
                     /locus_tag="VFF70_14900"
     CDS             complement(827..1612)
                     /locus_tag="VFF70_14900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZY46025.1"
                     /translation="MDVNLDQALDFAKKISREAGQLLTEYWHRGVVAEYKGAIDLVSE
                     ADRASEKLILDRIRSVYPDHAILSEESGSNQTSSPYTWIVDPLDGTTNFTHGHPDFAV
                     TIALQVKDAIELGVVIDPLRNELFAARKGQGSWLNDRRIKVSATEQLDRSLLVTGFPY
                     DRRTNPHNNLREDSAFLMMAQGVLRSGSAAIDLASVACGRVDGYWEFRLSPWDVAAGI
                     LLVNEAGGRVTNVEGEQLAVPHTDVVASNGLIHAEMMSVLRMM"
     gene            complement(1791..2120)
                     /locus_tag="VFF70_14905"
     CDS             complement(1791..2120)
                     /locus_tag="VFF70_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZY46026.1"
                     /translation="MESVNDFVSVAKITEIAAGTVKVVYVDDAPVGIANVGGRFYAFA
                     DVCTHDDGPVAEGPLVGCVIECPRHGAQFDIRTGAVLRLPAVVPIPVYDLKVDGDEIK
                     VSSSPRE"
     gene            complement(2152..2541)
                     /locus_tag="VFF70_14910"
     CDS             complement(2152..2541)
                     /locus_tag="VFF70_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZY46027.1"
                     /translation="MEDIYRDQIIDHYQNPRNYGTLEHPDISYEEDNPVCGDHIRIDL
                     KVIDGKVIDARFDGHGCSISQAAASMLTESIIGKPLDEVRQVGKEQILEMLGIPLGPV
                     RLKCALLSLKVLKAGAYGLHEWPGEEE"
     gene            complement(2589..>4052)
                     /locus_tag="VFF70_14915"
     CDS             complement(2589..>4052)
                     /locus_tag="VFF70_14915"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HZY46028.1"
                     /translation="GRIVASFEGEGCTISQASASYLMDEINGKTIDEALAIDHAELMD
                     GLGKEIVQQRTRCATLSLDTFRAALRKRDREQMISTLDVPKIRSDFPILNRQVHGKPL
                     VFLDSAASSQKPSVVIDAMDHYYRTTHANVHRGVHTLSEEATAQYEDARKRISKFINA
                     KSSREVIFTRNTTESINLVAYTWGRANIRSGDEILLTESEHHSNIVPWQLLAKETGAI
                     VRYLPIDVKGHLDLSALDLMLSKKTKIVSLAHMSNVLGTIYPIDEVIRRAHAVGAIVL
                     IDGAQSVPHLPTDVQAIDCDFLAFSGHKMCGPTGIGVLWGRRDLLEAMPPFLSGGDMI
                     RRVTFEGAEWNELPYKFEAGTPAIAEAIGLGVAVDYLSALNMNAVRAHEKELIAYALD
                     RLSEVPGLHLIGPDNPDRRGGVATFTLDEIHPHDLASSLDQEGIAIRAGHHCAMPLHQ
                     KLGISASSRASFYIYSTQEEVDKLVDGLYKAKSIFKR"
BASE COUNT          941 a         1184 c          990 g          937 t
ORIGIN      
        1 tagctgtcga agcatgtggg gcagtcatac agcgcctcct gcataaaatc gatgcgagat
       61 gccaacacgc gccgatcagc cgatcagcgc gtgcaatgat taaagcatgg attgaccgat
      121 ttcgccaacc caataaaaca aaatcataat gggaggatta aattgaatgc agcacgttca
      181 tcatctcggc gtgaatcgtg gacaaaaaaa cgaccgcgtg ggccgcggtc ggtgggctga
      241 aagcaatcat ttggtctcaa acagcgaacg aggcaaaccg gactgcctga taattccgag
      301 caacgtcccg attctcaatt catcatgatc tggaacaggc acagttgtag tcgtattctc
      361 tgcctgtctt tgcatgataa tgtggctgcc tctctgtcga atttcaacaa atccctgctt
      421 ggacatgatc ttgcaggctt ctcgcccaga gaggacgcgc agcctaggca tggcgcagcc
      481 tcgccacttg ctttaatttg atatttggcg cagcaggcat gatcgacatg atataggttt
      541 ctttcttcaa cctgcgccga atctctgaag gtgatgcgac ctcaaagaat ccctccaccg
      601 cttcaagcaa gttcaagcgt gcttgctcaa ccgtatcacc ctgactaacg acatcaagtt
      661 cggtgcaagt ggcgacgtaa ccatcgcctt cacgttcaat cacggcagtg agttgtagta
      721 ccttctccat agccatctcc tcttcttcga ttgtcattat aactcaccag aggttttacc
      781 gccgaagggc gcggggctgg cggcgcgtaa catgcggtgt aatcttctac atcattcgca
      841 acacactcat catctccgcg tgaatcaatc cattgctcgc caccacatca gtatgcggca
      901 cagccaactg ttcgccctcg acgtttgtta cgcgcccacc ggcctcgttc accaataaaa
      961 ttccggcggc cacatcccac ggactcagtc gaaattccca gtaaccatcg acgcggccac
     1021 atgccaccga agcgagatcg atcgccgcgc tgccggatcg cagtacgccc tgcgccatca
     1081 tcaagaaggc actatcttcg cgcagattgt tgtgcggatt tgtgcgtcga tcgtagggaa
     1141 agccggtgac taacaaactt cgatcgagtt gctcagttgc agaaactttt atgcgccgat
     1201 cattcaacca cgacccctgc cctttgcgtg cggcgaataa ttcattccgc agcggatcga
     1261 tcaccacgcc caactcgatc gcatccttca cttgcagcgc tatcgtcaca gcaaaatccg
     1321 gatgaccgtg tgtgaagttc gtcgtgccat ccagtggatc gacgatccaa gtataaggcg
     1381 atgacgtttg attcgatcca ctctcttcgg acaagatcgc gtgatcggga taaaccgatc
     1441 ggattcgatc aaggatcaat ttctcggacg cccgatcggc ttcgctgacc agatcgatcg
     1501 cccctttgta ttcggcgacg acaccacgat gccaatactc cgttaagagt tgccccgctt
     1561 cgcgagagat cttcttggcg aaatcaagcg cttgatcaag atttacatcc atatgaaagt
     1621 cacctcgcat gaaaaagggt cagcacctac cgacccattg agtctcagcg tcattgcgag
     1681 gagtgctctg cacgacgaag caatcccgct ttattttcat tgagattgct tcgtggcaca
     1741 tccctagagg gctgggccat ctagggactc cgctcgcaat gacggattga ttattcgcgc
     1801 ggcgatgaag acactttgat ctcatcaccg tccactttca aatcatacac cggaatcggc
     1861 acgacggccg gcaaacgcag aactgcaccc gtgcgaatat cgaactgcgc accgtggcgt
     1921 ggacattcga tcacacaacc cactaatggc ccttcggcga ccgggccgtc atcgtgcgtg
     1981 cacacatccg caaaagcgta gaaccgccca ccgacattcg caatgccgac gggcgcatca
     2041 tcgacataca caactttgac tgtccctgct gcaatctcag ttatcttcgc gaccgataca
     2101 aaatcattca ctgattccat ttgtcatttt ccattagtca tctgtcattg gctactcttc
     2161 ttcacccggc cactcatgca aaccgtacgc gcccgctttc aaaactttca aggacaacag
     2221 cgcgcatttt aaacgcactg gaccgagtgg aatacccaac atctccaaaa tttgctcttt
     2281 accgacctgg cgcacttcgt ccagcggctt gcctatgatc gactcggtca gcatcgaggc
     2341 ggcggcctgg ctgattgagc agccgtgccc atcaaaccgc gcatcgatca ctttgccatc
     2401 aattactttc aaatcgattc ggatgtgatc gccgcagacg ggattgtctt cttcgtatga
     2461 aatatcaggg tgttccagcg tgccgtaatt gcgtgggttc tgataatgat cgataatttg
     2521 atcgcgatag atgtcttcca taaatatcct catccgcccg taggtttgga acctccggga
     2581 ggataaattc agcgcttgaa gatcgatttg gctttataca agccatcgac caacttgtca
     2641 acttcttctt gcgtcgaata aatgtagaac gatgcgcgcg acgaagcgct gatgcccaac
     2701 ttctgatgca acggcatcgc gcagtgatgg cctgcccgaa tggcaatgcc ttcttgatca
     2761 aggctcgagg ccagatcgtg cggatgaatc tcatcgagcg taaacgttgc cacgccgccg
     2821 cgtcgatcgg gattatccgg cccgatcaag tgcaaacccg gcacttcact caatcgatcg
     2881 agcgcatacg cgataagctc tttttcgtgc gcgcgaacgg cgttcatgtt caatgccgac
     2941 aagtaatcaa cggcgacacc tagaccaatc gcttcggcaa tcgccggcgt gcctgcctca
     3001 aatttatatg gcagttcatt ccactcggca ccttcaaacg tcacacgccg gatcatatcg
     3061 ccgccactca aaaacggcgg catcgcttcc aagagatcac gtcgccccca caagacgcca
     3121 atgcctgtcg gcccgcacat cttgtggccg ctaaacgcca gaaaatcgca gtcgatcgct
     3181 tgtacatcgg tcggcaaatg cggcacgctc tgcgcgccat cgatcaagac gatcgcgccg
     3241 acagcatgcg cacggcggat cacttcgtcg atcggataga tcgtgccaag cacattcgac
     3301 atgtgcgcca gcgaaacgat cttcgttttc ttactcaaca ttaaatcaag cgccgacaga
     3361 tccagatgac ctttgacatc gatcggcagg taacgaacaa tcgcgcccgt ttctttggcc
     3421 agcaactgcc acggcacaat gttgctgtga tgctccgact cggtcagcag aatctcgtca
     3481 cccgatcgga tgttggcgcg gccccaagta taagccacca aattaatcga ttcggtcgtg
     3541 ttgcgcgtaa agatcacttc gcggctggat ttcgcattga tgaacttgct gatccgtttg
     3601 cgagcatctt cgtattgtgc ggtcgcttct tcgctgaggg tatgcacacc gcgatgcacg
     3661 ttcgcgtgcg tggtgcgata gtagtgatcc atcgcgtcga tcacgaccga cggcttttgc
     3721 gatgaagccg cgctgtcgag aaacactagc ggcttgccat gcacctgccg attgaggatc
     3781 gggaagtccg atcggatttt ggggacgtcc aaagttgaga tcatttgttc gcggtcgcgc
     3841 ttgcgcaggg cggcgcggaa cgtgtccagc gacaacgttg cgcagcgggt gcgctgctgg
     3901 acgatctctt tgccgaggcc gtccatcaac tcggcgtgat cgatcgccag cgcttcgtcg
     3961 atcgtcttgc cgttgatctc gtccatcagg tacgaggccg atgcctggct gatggtgcag
     4021 ccttcgccct cgaatgaggc gacgatgcga cc
//
LOCUS       DASRTJ010000738         1814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_215228,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000738 DASRTJ010000000
VERSION     DASRTJ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215228"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFF70_14920"
     CDS             <1..837
                     /locus_tag="VFF70_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY46029.1"
                     /translation="LAAAWGGTFVMVKDAVAVMPVFTFLTLRFFVASITLLSISQPRR
                     LRQLDRRAALLSILAGALFAAGYGFQTLGLQTTGPGQVGFITGLSVVLVPIGAAIIWR
                     VRPNRSAIVGVACAVIGLALLTGVFNGQPIAPGAVWVMLGTLGFAGQILVVGALPRQI
                     DPRVLATLQVFSTAVGCGIISILIEKPIVPASPTVWGAILVTGIIVSALGVILQTWAQ
                     SFTSATHTALIFATEPVFAAITGFILLSERLSADQMIGCGLILIGMLISELGNRESTL
                     PL"
     gene            882..>1814
                     /locus_tag="VFF70_14925"
     CDS             882..>1814
                     /locus_tag="VFF70_14925"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY46030.1"
                     /translation="MLNDALQYAEAHRDQFLSELKEFLSIPSISAVAEYQKDLQRAAQ
                     WLADHFTSIGLHHATVHQTDGHPIVTADWLHAGADQPTVLIYGHYDVQPIDPIELWQS
                     DPFKPEVRGDYLFGRGTADDKGQVFIHAKVIESYLKTTGKLPVNVKIVVEGEEEIGGP
                     NLDVFVKAHLAELNADVCLISDSHMLRPDQPCIIYGLRGLTYIEIEVTGPGQDLHSGS
                     YGGAVYNPIQALVEMLASLKNKNGHITVPGFYDKVRPLKKDERNVLARVPFSKKQFMR
                     EASVKKLWGEKGYTPVEQTAARPTLELNGIWGGWT"
BASE COUNT          388 a          491 c          512 g          423 t
ORIGIN      
        1 ttggccgccg cctggggcgg aacgtttgtc atggtcaaag acgcggtcgc ggtgatgccg
       61 gtctttactt tcctcacgct acgtttcttc gtcgcctcga tcacactgct atcgatttct
      121 cagccgcgcc gattgcgaca actcgatcga cgcgcggcac tgctcagcat tctggccggc
      181 gcattattcg ccgcgggcta cggctttcaa acgctcggct tgcagacaac cgggccgggt
      241 caagtgggtt tcatcaccgg tttgtcggtg gtcctggtgc cgatcggcgc ggcgatcatt
      301 tggcgcgtgc gcccaaatcg atcagcgatc gtcggcgtgg cctgcgcggt gatcggcttg
      361 gccttgttaa ccggcgtatt caacggccaa ccgatcgcgc ccggcgctgt ttgggtgatg
      421 ctgggcacgc ttggttttgc cggacagata cttgtcgtcg gcgcgctgcc gcgccagatc
      481 gatccgcgtg tgctggcgac acttcaagtt tttagtacag ccgttgggtg cggaatcatt
      541 tcgatcctca ttgaaaaacc gatcgttcct gctagcccga cggtttgggg cgcaattttg
      601 gttacgggta tcatcgtcag tgctctaggt gtcatcctgc aaacgtgggc gcaatctttc
      661 acgtcggcca cacataccgc tttaatcttc gctaccgaac cggtctttgc cgcaatcacc
      721 ggtttcattt tgctgagcga gcgcttaagc gcagatcaaa tgatcgggtg cggattgatc
      781 ttgatcggga tgttgatcag cgaattgggc aatcgcgaat cgaccttgcc gctttgatga
      841 tcgcgcctta taattaattt acacattatt caggagtcac catgcttaac gatgctttgc
      901 agtacgcgga agcacatcgc gatcagtttt tgtcggagct caaagaattt ctgtcgatcc
      961 ccagtatcag cgcagttgcc gagtatcaaa aagacttaca gcgcgcggcg cagtggctgg
     1021 cggatcactt cacctcgatc ggtttacacc acgccactgt tcatcaaacc gatggacacc
     1081 cgatcgtgac cgccgactgg ctgcacgccg gagccgatca accgacggtg ttgatctacg
     1141 gccattatga cgtacagccg atcgatccga tcgagttgtg gcagtccgat ccgttcaagc
     1201 cggaagtgcg cggcgattat ttgttcggac gcggcacagc ggatgataag ggacaagttt
     1261 tcatccacgc caaagtgatc gagtcgtatc tcaagacaac gggcaagtta cctgtcaatg
     1321 tcaagatcgt cgtcgagggc gaagaagaaa tcggcggacc caatctcgac gtgttcgtta
     1381 aagcacatct cgccgaatta aacgccgatg tgtgtttgat cagtgactcg cacatgctgc
     1441 gacccgatca accgtgcatc atctacgggc tgcgcggcct aacgtacatt gagatcgaag
     1501 tgacgggccc cggtcaagac ctgcattcag gatcatatgg cggcgcggtc tacaatccga
     1561 ttcaagcgct ggtcgaaatg ctggcttcgc tgaaaaataa gaacggtcac attaccgtac
     1621 cgggtttcta tgacaaggtg cgcccattaa agaaagacga gcgtaatgtg ctggcgagag
     1681 tgccgtttag taaaaaacaa ttcatgcgcg aagcgagtgt gaaaaagttg tggggcgaaa
     1741 aagggtacac gcccgtcgaa caaaccgccg ctcgaccgac gctggaactc aatggcattt
     1801 ggggcggctg gacc
//
LOCUS       DASRTJ010000739         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_215908,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000739 DASRTJ010000000
VERSION     DASRTJ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215908"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFF70_14930"
     CDS             complement(<1..391)
                     /locus_tag="VFF70_14930"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HZY46031.1"
                     /translation="MIDPSSTLNDLKEDAIHRLTQADVDSPHISAEVILAHAVSLTRS
                     QLLARSDFKLNPDQLARFQIDLDRLVKGEPLAYVIGHREFYDLDLITDERALIPRPET
                     ECLVEKAIDLLTAHPHPLIADIGTGSGA"
     gene            complement(447..1523)
                     /gene="prfA"
                     /locus_tag="VFF70_14935"
     CDS             complement(447..1523)
                     /gene="prfA"
                     /locus_tag="VFF70_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZY46032.1"
                     /translation="MELLEKLAGIETRYEELNNLMADPAVIADYNRLREVGQERNNLQ
                     PMVEAYQEYRGALQHLDQAKSMVASETDPEMAAMVQAEVTDLETKIAAMETNIKSLLV
                     PKDPRDDKNVIVEIRAGTGGDEAGLFAAELYRMYSRYAENRRWKPELLSSNETGVGGF
                     KEVIFMIKGKGTYSRMKYESGVHRVQRVPVTESSGRIHTSTVTVAVLPEVDEFEIEIL
                     DKDIEIEVYRSQGAGGQNVQKNATAVRIRHLPTGIVVQCQDERSQLQNRLRALSILRA
                     RLYEQEEDKRQKEQTDSRRSQVGSGERSEKIRTYNYPQSRVTDHRVDITSYQLDSVLN
                     GDLDLFIDELITRDQAEKLQAAGF"
BASE COUNT          300 a          399 c          435 g          391 t
ORIGIN      
        1 tcgcgccact accggtgccg atgtccgcga tgagtggatg cggatgcgct gtgagtaaat
       61 cgatcgcttt ttcgacaagg cattcggttt caggccgcgg aatcagcgcg cgctcatcgg
      121 tgatcagatc gagatcgtaa aactcgcgat ggccgatgac gtaagctaga ggctcaccct
      181 taacaagtcg atcgagatcg atctggaagc gtgcgagttg atcgggattg agcttaaagt
      241 ccgatcgagc cagcagctgc gatcgggtga gtgacacagc gtgcgccagg atcacttcgg
      301 cactgatgtg tggcgaatcg acatcggcct gagtcaaacg atggatcgca tcttctttga
      361 gatcgttcaa ggttgacgaa ggatcgatca tgatgaagta cgtagtgatg agtaaatact
      421 aacgtgcact aattactctt cacccatcaa aaacctgcgg cctgaagttt ctctgcctga
      481 tcacgtgtga tgagttcgtc gatgaagaga tcgagatcgc cattgagcac actgtcaagt
      541 tgatacgatg tgatgtcgac gcgatgatcg gtgacgcggc tttgcggata attgtacgtg
      601 cggatttttt cgctgcgctc gccgctgccc acttgtgatc ggcgcgagtc ggtttgttct
      661 ttctgccgct tgtcttcttc ctgctcatat aagcgcgccc gcaggataga cagggcacgc
      721 aatcgattct gcaattgcga gcgttcgtcc tggcattgca cgacgatgcc agtcggcaga
      781 tgacggatcc gcaccgcggt ggcgttcttt tgcacgttct gtccgcccgc accttgcgat
      841 cgatagactt cgatctcaat atccttatcc agaatttcga tctcgaattc atcgacttcc
      901 ggcaagacgg cgacggtgac ggttgaagtg tggatgcgtc ctgatgattc ggtgaccggc
      961 acgcgctgga cgcgatgcac gccgctttca tatttcatac gcgagtacgt gcctttgcct
     1021 ttgatcatga agatcacttc tttaaagccg ccgacgccgg tctcgttgct cgataagagt
     1081 tcgggtttcc agcggcggtt ttccgcgtag cgtgagtaca tccgatagag ttcggcggcg
     1141 aacaggcccg cttcatcgcc gccggtgccg gcgcgaatct cgacgatcac atttttgtcg
     1201 tcgcgcggat ctttaggcac gagcagcgat ttgatgttgg tttccatcgc cgcgatcttc
     1261 gtttcgagat cggtgacctc tgcctgcacc atggcggcca tctccggatc ggtttcggag
     1321 gcgaccatcg acttggcttg atcgagatgc tgcaatgcgc cgcgatattc ttggtacgcc
     1381 tcgaccatcg gctggaggtt attgcgttcc tgccctactt cgcgcaggcg gttgtaatcg
     1441 gcgatgacgg ccggatcggc catcaagtta ttcagttctt cgtagcgggt ttcgatgccc
     1501 gctagttttt caagaagttc catga
//
LOCUS       DASRTJ010000740         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_178438,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000740 DASRTJ010000000
VERSION     DASRTJ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178438"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..2190
                     /locus_tag="VFF70_14940"
     CDS             208..2190
                     /locus_tag="VFF70_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZY46033.1"
                     /translation="MFGGGRGPAMMMEREVLKPKNVGETLKRFGTYFRPYVGVLLLVA
                     TFVIISTWAQVTTPDLIGQAVDCYLTPTAAQQFGGFTGSGVTQSAADNCWYDPDPTHT
                     RTSDQNIAGLGGLILKIIGLFLLSAILTGLTFFGMSWAGQHVLRRLRTEVFEHIHRLS
                     LSYYAEHEAGDVMSRITNDADTIQQALSFALINVIGGALLLIWIAYTMMRLSFAYALI
                     ALVAVPFMWFATTWFSGQARKAFRKSRLEMGSVNAELQQSISSVREVQAFNRERENIE
                     NFRDVNAANRDANIRAVAFTAALPPTLEALGFVAMALVVGVGGLLILRNQDLFGTAIS
                     IGLIITFLGYVQRFNQPIQQIAVLWTNIQSAIAGGERIFSLLDEVPAVQDKSDARPMP
                     TINGTVEFDAVHAEYEKGVPVLRGINFTAQPGQMIAVVGPTGAGKTTIINLVPRFYDV
                     TGGAVKIDGVDVRDVTAASLRQQIGIVLQDSFLFSDTVLNNIRFGRPEATEEEVIAAA
                     KLARADTFIDRLSDGYQTILGERGSGLSQGQRQLLSIARAALANPRILILDEATSSVD
                     TRTEKLIQDALEKLLHGRTSFVVAHRLSTIKHADQIFVLKDGEIIERGKFNELLNAHG
                     FFYDLYMSQFRRQEQIEGATQPSGNGQHATTVTATA"
     gene            complement(2273..>3256)
                     /locus_tag="VFF70_14945"
     CDS             complement(2273..>3256)
                     /locus_tag="VFF70_14945"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZY46034.1"
                     /translation="RAGLIQIATSLIPYLVLWYAMIRAYDVSPLLTLGLALFAAGSLV
                     RIFIIVHDCGHESFFESQKLNDFVGSILGALVFTPYFEWRHAHAIHHATAGNLNRRGV
                     GDVFMLTVKEYNSKNWLGRLGYRLYRNPLIMFGIGPIFMFMIVHRFPARNSRPRERNS
                     VWLTNLAILGFSVIMSLLIGFEKYAVLMVLTLGLAGIAGVWMFYVQHQFEGTYWEPQV
                     EWTYVEAALRGSSYYKLPKILQWFTGNIGLHHIHHLSPRIPNYNLQACHDSNPPLQDV
                     PTITLLPSFKSMTFKLWDEEHHKLVGFNAAKKLQKAEMRTNAKRVVQEPTS"
BASE COUNT          762 a          855 c          930 g          709 t
ORIGIN      
        1 cacgaagaat tgatcgaaac gagcgagatt tacgcgcaga tttacagctc gcagttgctg
       61 gaagatgtgc atctcgatga gcagaaggtt gctgagtctg tgaaggttgc ttaattgaaa
      121 cgtggtgcgt gatgcgtaaa acggaacacg cagcacgtaa tagcgatagg cgataggcca
      181 tctgctatcg gccattagga ggcaagtatg tttggtggtg gacgcggacc tgcgatgatg
      241 atggagcgcg aggtccttaa accgaaaaat gtcggcgaga cgttaaaacg ttttggaact
      301 tacttccggc cgtatgtggg cgtgctgctg ttagtcgcca catttgtgat catctcgacg
      361 tgggcgcaag tcacgacgcc ggacttgatc ggacaggctg tcgattgtta cttgacgccg
      421 acggcagcgc agcagtttgg cggcttcacc ggttcgggcg taacacagag cgcggcagat
      481 aactgctggt acgatcccga tccgacgcat acacgcacgt ccgatcaaaa catcgccgga
      541 ctgggcggct tgatcttgaa gatcatcgga ctgtttctgc tgagcgccat tctgaccgga
      601 ctgactttct tcggcatgag ttgggcgggc cagcacgtgc tgcgccgctt gcgcaccgaa
      661 gtctttgaac acattcatcg cttatcgctg agctattatg cggagcacga agcgggcgat
      721 gtcatgagcc gcatcacgaa tgatgcagac acgattcagc aagcgctgag ttttgcgctc
      781 atcaatgtga tcggcggagc gctgctgctg atctggatcg cgtacacgat gatgcgcttg
      841 agctttgctt acgcgttgat cgcgttggtg gcggtgccgt tcatgtggtt tgcaacgacc
      901 tggttctccg gccaggcgcg caaagcattt cgcaagagcc gtttggaaat gggcagcgtg
      961 aacgcggaat tgcagcagag catctcaagc gtgcgcgaag tgcaggcgtt taaccgcgag
     1021 cgggaaaata tcgaaaactt ccgcgatgtg aatgcggcca atcgcgatgc caacattcgc
     1081 gcagtcgctt tcacggccgc gctgcccccc acgctggaag cgctgggctt tgtcgccatg
     1141 gcattggtgg tcggagtcgg cggtttgttg atcctgcgca atcaagatct cttcggcacg
     1201 gccatctcca tcggtttgat catcaccttc ctcggctatg tgcagcgctt caatcaaccg
     1261 attcagcaga tcgccgtgtt atggacgaat attcagagcg cgatcgcagg cggcgaacgg
     1321 atcttcagtt tactcgatga agtcccggcc gttcaagata aatctgacgc gcgaccgatg
     1381 ccgacgatca atggcacggt cgagttcgac gcagtccatg ccgaatacga aaagggtgtg
     1441 ccggttttgc gcggcatcaa tttcacggct caacccggac aaatgatcgc ggtcgtcggg
     1501 ccgacgggcg cgggcaagac aacgatcatc aatcttgttc cacgtttcta cgatgtaacc
     1561 ggcggcgcag tcaagatcga tggtgtcgac gtgcgcgacg tgacggcggc cagcctgcgc
     1621 caacagatcg gtatcgtgct gcaagatagt ttcttgttca gcgatacggt gctgaataac
     1681 attcgcttcg gccggccgga agcgacggaa gaggaagtga tcgcggcggc gaaactggcg
     1741 cgggccgata cgttcatcga tcggctgtcg gatggttatc aaacgatctt gggcgagcgc
     1801 ggcagcggct tgagccaagg ccagcggcaa ctgctctcga tcgcgcgtgc ggcgttagcg
     1861 aatccgcgca tcttgatctt ggatgaagcc acctcgagtg tggatacgcg caccgagaaa
     1921 ttgattcaag acgccttgga gaagttgctg catggccgga cgagttttgt cgtggcgcac
     1981 cgcttgagca ccatcaagca cgccgatcag atctttgtgc tcaaagatgg tgagatcatc
     2041 gagcgcggca agttcaatga actgctcaac gcgcacggct tcttctacga tctgtatatg
     2101 agccaattcc ggcggcagga acagattgag ggcgcaacac aaccatcggg taatggacag
     2161 cacgctacaa ccgtaacggc aacagcgtga tcgatcaatc aactaaataa aagaagtccg
     2221 acttcgatgc tgatgaagtc ggacttcttg tttgtatcaa ctaagattta cgttagctcg
     2281 ttggttcttg taccacgcgc ttcgcgttcg ttcgcatttc agccttttgt aatttctttg
     2341 cggcattgaa accaaccagt ttatgatgct cttcatccca caacttaaaa gtcatggatt
     2401 tgaaactggg cagcaaggtg atcgttggca catcttgcag cggcggattt gagtcatgac
     2461 aagcttgcaa gttgtaattc ggaatgcgcg ggctaagatg atgaatgtga tgcagcccga
     2521 tgttgccggt gaaccactgt agaatcttgg gcagcttgta atacgaactg ccgcgcagcg
     2581 ccgcctcgac ataggtccat tcgacttgcg gctcccagta tgttccctcg aattgatgct
     2641 ggacatagaa catccacacg cccgcaatac cggccagccc cagcgtcaaa accatcagca
     2701 cggcgtattt ctcaaagccg atgagcaaac tcatgatcac gctgaagcct aaaattgcca
     2761 ggttggtcag ccagacactg ttgcgttcgc gcggccgcga gttgcgcgcc ggaaagcgat
     2821 gcacgatcat gaacatgaag atcggaccga tgccgaacat gatcagcgga ttgcggtaca
     2881 accgatagcc caaccgcccc agccagttct tgctattgta ttccttcacc gtcagcataa
     2941 acacatcgcc cacgccgcgc cgattgagat tgcccgccgt cgcatgatga atggcatgcg
     3001 catgccgcca ttcgaaatac ggcgtaaaga ccagtgcgcc caagatcgat ccgacgaagt
     3061 cgtttaattt ttgtgactca aagaatgact cgtggccgca gtcatggaca atgatgaaga
     3121 tccgcaccag cgaaccggcc gcaaacaggg ccagccccaa agtcaaaagc ggcgagacat
     3181 cgtaagcacg aatcatggca taccacaaaa ccagatacgg gatcaggctc gtggcgattt
     3241 ggatcaaacc ggcgcg
//
LOCUS       DASRTJ010000741         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_178443,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000741 DASRTJ010000000
VERSION     DASRTJ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178443"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFF70_14950"
     CDS             <1..395
                     /locus_tag="VFF70_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZY46035.1"
                     /translation="IGVMGDPGILDAVAKDPLGIGYNNLGYAFDNVTGTSVPGTVVLP
                     IDKNNNGKADTDETIETKQQAVSAVAAGKYPSPPARPLNLVTKGKPTSLTQVFIAWVL
                     SDGQKFVDGAGFVPLAASEINSAQAKIK"
     gene            417..1349
                     /gene="pstC"
                     /locus_tag="VFF70_14955"
     CDS             417..1349
                     /gene="pstC"
                     /locus_tag="VFF70_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZY46036.1"
                     /translation="MRSLRQLKDRIAGRLFFSMTLLPFVLLAVMIVLLLVRARPLLET
                     KPIGDLLFGEVWKPDQGEFGFWPFITGTVWVTTVAMIVAIPVCLLCSIYLSEYAGSRV
                     RAIMKPMLDLLSGIPSVIFGVWGVIAVVPFVQDVVAPFASKFLSFVPIFNTRNNPTGY
                     SIIAGGFVLGVMVTPVIIAVGYEVMQTVPHGLREASLSVGATKWQTIKHTVLPKALPG
                     IIAGVVLGFSRAFGETMAVLMVVGNVPKVPGSIFDPAYPLTALIANNYGEMMSVPLYE
                     SALLGAALILLLVVVIFNVLSALALHRIVGRSIG"
     gene            1346..2206
                     /gene="pstA"
                     /locus_tag="VFF70_14960"
     CDS             1346..2206
                     /gene="pstA"
                     /locus_tag="VFF70_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZY46037.1"
                     /translation="MKEKLARRHIEEGIFKVLMIGSFLIVAGSLGLILLTVVVKGLPG
                     LTIEMLTQSPKGGFYLGKEGGIANAIVGSLYLAGGATILALIISLPIVLYLAMYVRRS
                     RWAAWVRLSLDVLWGIPSIVYGAFGFIVMLALGLRASLLGGIIVLTILELPIMTRAMD
                     EIIRLMPFELKEAAYALGATRLEIALSVIVRQALPGILTAMLLAFGRGIGDAASVLFT
                     AGYTDRMPESLLRPVASLPLAVFFQLGTPYPEVQQRAYTAALILTFIILIISLGSRWL
                     SRRMSRNVIK"
     gene            2243..2998
                     /locus_tag="VFF70_14965"
     CDS             2243..2998
                     /locus_tag="VFF70_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZY46038.1"
                     /translation="MEAHIQVRDLNVDYGDHSALKNISVDIPKGQVTVILGPSGCGKT
                     TLLKSFNRLIEMADGVKVSGQILIDGVDIYDPAVEVTDIRKKAGLLAQRPFPLPMSIY
                     DNVAYGMRIHRMKRGKKLNQAVQHYLEMAGLWDEVKDRLHAPATRLSIGQQQRLCLAR
                     GLAVEPEIMLADEPTSALDPISARHIEQRLLELSQNYTVVLVTHILRQAKRLADYVIF
                     MYLGELIEHGPAHEVFEHPREERTREYLAGAIG"
     gene            3206..3400
                     /locus_tag="VFF70_14970"
                     /pseudo
     CDS             3206..3400
                     /locus_tag="VFF70_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
     gene            3452..4078
                     /locus_tag="VFF70_14975"
     CDS             3452..4078
                     /locus_tag="VFF70_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46039.1"
                     /translation="MEVAVILVGAVIAVFGAMMITLALWPSGSKSAPQPPIDTAAVAI
                     ASDATEVRTADDQSRLTGIQRAQARKSAEAAALAESVSLIERHEEVSRPNIPLQLETL
                     EIISAQIERLQTEYLRVGEDRKRLAQELLTSWLIEKFDETPGRLKADTKKEAQDLRQQ
                     LIKTSGEFERVEFRLTSLVNLQARLDDPRVTQQIDDLVYAVKQLARER"
     gene            4235..>5230
                     /locus_tag="VFF70_14980"
     CDS             4235..>5230
                     /locus_tag="VFF70_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46040.1"
                     /translation="MKTKQWSILLLVCVSIGTVGLLLSHMYSPQTKGSVQVLDRSATV
                     LPQAVKPELSSAQQPAFPDSDAQVTATGPFTISLPENIPTILLTDKPIEPSSLTGGVG
                     NLPYVPPSPDTKPEFEMEPAPKSDTSAGSSAPPAAPALVLGTMPTPTLTFNAMDYTTN
                     GAGHPPDTNGDVGLNHYMEAVNTSIGIYTKTTGAQATAFTFNSFWSGAGTGTACDTGG
                     ILDGPSNQGDPIVLYDTIRQRWIFMDFAWTTPNLINGPYYFCFGVSQTSNPLGNYWRY
                     AIRADDAARPWLPDYPKGGVWPDGLYFSANMFDCLNASCSSAIREDARAYAFNLQK"
BASE COUNT         1141 a         1302 c         1498 g         1289 t
ORIGIN      
        1 gtatcggtgt gatgggcgat ccgggcattc tggatgccgt tgccaaagat ccactgggca
       61 ttggctataa caatttaggc tatgcgtttg acaatgtcac gggtacatct gtgcccggca
      121 cagtggtgct gccgatcgac aagaacaaca acggaaaggc ggataccgat gaaacgattg
      181 agactaaaca gcaagccgtt agcgcagtcg ctgctggcaa gtatccttca ccgccagccc
      241 gtccgcttaa cttggtgact aagggcaaac cgaccagcct gactcaggtc ttcatcgcgt
      301 gggtgctcag cgacgggcaa aaatttgttg acggcgcggg ctttgtgcca ttggctgcta
      361 gtgaaataaa ctcggcgcaa gcgaagatca aatagtgtcg gcacacaggc catggaatga
      421 gatctcttag acagctcaaa gatcgaatag cgggtcgatt gtttttctca atgaccttac
      481 tgccttttgt gttgctggcg gtaatgattg tgttgttgct ggtgcgcgcc cggccattgc
      541 ttgagacgaa accgatcggc gatctgttgt ttggcgaagt gtggaaaccc gatcaaggtg
      601 agttcggttt ttggccattt attacaggca cggtgtgggt cacgaccgtg gcaatgatcg
      661 tcgccatacc ggtttgcttg ttgtgttcaa tttatctatc ggagtatgcc ggatcgcgcg
      721 tgcgggcgat tatgaagccg atgctcgatc tgctgagcgg tattccttcc gtcatcttcg
      781 gagtgtgggg cgtgatcgcc gttgtgccgt ttgttcagga cgtcgtggct ccattcgcca
      841 gtaaattctt gagtttcgtt ccaatattca acacccgtaa taatcccacc ggctacagta
      901 tcatcgccgg tggatttgtc ttgggcgtta tggtcacacc ggtgattatc gccgttgggt
      961 atgaagtcat gcagacggtg ccacacggtt tgcgtgaagc ttctctctcg gtaggtgcaa
     1021 caaaatggca gacgatcaag catacggtgt tgcctaaagc tctgcctggt atcatcgccg
     1081 gtgtcgtgct gggattttca cgtgcctttg gtgaaaccat ggctgtgttg atggtcgtcg
     1141 gcaacgtgcc caaagttcca ggatcgatct ttgatccagc ttatccgttg acggcgctca
     1201 ttgccaacaa ctatggcgag atgatgtccg tgcctttata tgaatcggcc ctgctgggtg
     1261 cggcgctgat tttgctgctg gtcgtggtga tcttcaacgt gctttcggct ttggcgctcc
     1321 atcggattgt cgggagatcg atcggatgaa ggaaaaactc gcgcggcgtc acatcgaaga
     1381 aggtattttc aaggtcttga tgatcggatc gtttctgatc gtcgcgggta gtctgggctt
     1441 gatcttattg acggtcgttg ttaagggctt gcccggcctc acgatcgaaa tgttgacaca
     1501 atcacctaag ggcggctttt atctgggcaa agagggcggt atcgcgaatg cgatcgtcgg
     1561 ctcattgtat ctggccggcg gcgcgacgat cttggccttg atcatcagtt tgccgatcgt
     1621 tttgtatctg gcgatgtacg ttcgtcgatc gcgctgggct gcctgggtga gattgtcgct
     1681 cgatgtgttg tggggcattc catcgatcgt gtatggtgcg tttggtttca ttgtgatgct
     1741 ggcgcttggc ttgcgagctt cgctgctggg cgggattatc gttctgacga ttttagaatt
     1801 gccgatcatg acgcgagcaa tggatgaaat tattcgcctg atgcccttcg agttgaaaga
     1861 ggcggcgtat gcgctgggtg cgacgcgctt ggaaattgcg ttgagcgtga ttgtgcgcca
     1921 agcgctgcct ggtattttga cggcgatgct gctggcattc gggcgtggga tcggcgatgc
     1981 ggcctccgta cttttcacgg caggttatac cgatcgaatg cctgagtcgt tgttgcgtcc
     2041 cgtggcctcg ctgccgcttg ccgtattctt tcaattgggc acgccgtatc ccgaagttca
     2101 gcagcgcgct tatacggcgg cgctgattct aacgtttatt atcctgatca tcagtcttgg
     2161 ttcgcgctgg ttgtcgcggc gcatgtcgcg gaatgtcatc aaatgaatta accacggaca
     2221 cgtgtggtta taggaaaacc ttatggaagc acatattcaa gttcgcgatc tcaacgtcga
     2281 ttatggcgat cattctgcgc tgaaaaatat ttcggtcgat attcccaagg ggcaggtcac
     2341 cgttatcctt ggaccttcgg gttgtggcaa gacgacgctg ctcaagagtt ttaatcgctt
     2401 gatcgaaatg gctgacggcg taaaagtgtc cggtcagatt ctgatcgatg gggttgacat
     2461 ttacgatccc gccgttgagg tgacggacat acgcaagaag gccgggcttc tcgcccagcg
     2521 accatttcca cttccgatgt cgatctatga taacgtggct tacggtatgc ggatccatcg
     2581 gatgaagcgc ggcaagaaac tcaatcaggc tgtgcaacac tatctggaaa tggccggttt
     2641 gtgggatgag gtcaaagatc gattgcatgc accggctacc cggctctcga tcggtcaaca
     2701 gcagcgctta tgtttggcgc gcggcttggc cgtcgaaccg gagattatgc tggccgacga
     2761 accgacctcg gcgctcgatc ccatttcggc gcgccacatc gagcagcgcc tgcttgaatt
     2821 gagtcagaac tatacggtcg ttttagtgac acacattttg cggcaagcca agcgtttggc
     2881 cgattacgtg atcttcatgt atctcggcga attaatcgag cacggtccgg cgcatgaagt
     2941 ctttgagcat cctcgcgaag aacgcacgcg cgaatatttg gcgggcgcca tcggctgact
     3001 gtttccgatt tgggcatttg aaaaaagaat gagagaatga gagacgggag acacagctgc
     3061 ctgtctctta tccattataa cggggactta atggagacca ttgaatcgca gatcgatccg
     3121 aacgctgagc agtttgaagc gaatgccggg cacaatcaac gcttgccgat gaactgcgcg
     3181 atcgaaagag ccgctgcttg atccacagca acaggcggaa ttcaagcgac cgatccttga
     3241 aaagtacgaa cgcgaaggca gtccatatta ttcaacggcg cgcctgtggg atgatggcat
     3301 tctcgatccg atcgagacgt ggcgcgtgtc gggcctagct ttgtcggcct cattgaatgc
     3361 gccgatcgag aaaacggaat atggcgtatt tagaatgtaa atctagcgat tttatagtac
     3421 aatacaggca gagggaacta aaaggacgat tatggaagta gcagtgattc tggttggcgc
     3481 tgtcatagct gtgtttggcg cgatgatgat cacgctggcg ttgtggccga gcggcagtaa
     3541 gtctgcgcct cagccaccga tcgatactgc ggctgtggcg atcgcgtctg atgcgaccga
     3601 ggtgcgcaca gccgatgatc aatctcgatt gaccggaatt caacgggctc aggcgcgaaa
     3661 atcagccgaa gctgcggcct tggccgaatc cgtgtcactg atcgaacggc atgaagaagt
     3721 ttctcggccc aacattccgc tgcaattgga gacgctcgaa attatttccg cacagatcga
     3781 acggctgcaa accgaatatt tgcgtgttgg cgaagaccgc aagcgtttag cacaggaatt
     3841 gttgacaagc tggctcattg aaaaatttga tgaaaccccc ggccgcttga aggctgatac
     3901 gaagaaagaa gcgcaggatc tgcgccagca gttaatcaag accagtggcg aattcgagcg
     3961 cgttgagttt cgcctgacat cgttggtaaa cctccaggca cggctcgacg atccgcgcgt
     4021 cacccagcaa atcgacgacc tcgtttacgc agtgaagcag ttggcgcgtg aacgctaacg
     4081 acatctgggt aaatgatttc tccttcgata ttacagcttt gctcacaatc atcaatagaa
     4141 ttccgatcgc gaatcttggc aatatcgcgg ttcaaattat ttgcgcttac caagagttag
     4201 gcattcgcgc gagtgcagcc tggaagggag ccaaatgaaa acaaagcaat ggtcaattct
     4261 gttactggtg tgtgtctcga tcggaacggt gggtctgttg ctgagccata tgtactcgcc
     4321 acaaacaaag ggaagtgtac aggtgctcga tcgatcagcg accgtgttgc cgcaggcggt
     4381 caagcctgaa ttatcgtcgg cgcagcaacc cgcgtttccg gatagcgacg cgcaggtcac
     4441 ggcgacggga cccttcacca tctctctgcc ggaaaatatc cccacgatct tattgaccga
     4501 taagccaatc gagccaagct cgctaacggg tggtgtaggg aatttgccct acgtgccgcc
     4561 ctcgccagac acaaaaccgg aatttgaaat ggaacctgct cccaagagcg acacaagtgc
     4621 gggtagcagt gctccacctg ccgcgcccgc gctcgtcctg ggtaccatgc ccaccccgac
     4681 gttgaccttt aatgcgatgg attacaccac gaatggagcc ggtcatcccc cggataccaa
     4741 tggcgatgtc ggcctcaatc attacatgga agctgtcaac acctcgatcg gcatttacac
     4801 caaaacgaca ggcgcgcagg ccacagcctt tacattcaat tcgttctggt cgggcgcggg
     4861 cacgggcaca gcgtgcgata cgggtggtat cctggatggt cccagtaacc agggtgatcc
     4921 gatcgtgttg tatgacacca tcaggcagcg ctggatcttc atggacttcg cctggacgac
     4981 cccgaacctt atcaacggcc cgtactactt ctgtttcggc gtctcacaga ctagcaatcc
     5041 actgggcaat tactggcgat atgccattcg tgccgatgat gcggcgcgtc cgtggctgcc
     5101 ggactatcct aaaggcggcg tgtggccgga tggcttgtat ttctcggcca atatgtttga
     5161 ttgtctgaat gccagctgct catcggcgat ccgtgaggat gcacgtgcct atgcgttcaa
     5221 tctgcagaag
//
LOCUS       DASRTJ010000742         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_217221,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000742 DASRTJ010000000
VERSION     DASRTJ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217221"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1514
                     /locus_tag="VFF70_14985"
     CDS             270..1514
                     /locus_tag="VFF70_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HZY46041.1"
                     /translation="MTTNKLALKILYIEDDANSRRLVQRVLEVEGYEVHVAENGLAGL
                     EMARRLRPALVLTDVNMGGMTGHEVATRLKEMPETRHAPVIAITSNVLQTDRELALVA
                     GCDGYITKPIDVDALPRLIERFLAGERESLPDNVRLLRLEEYSHTLVNRLESTVAELQ
                     TANDELHRMDKMKKDFVVLSSHELRTPTTLIYGYINLLKMETKDLPLGDRLMDMINRI
                     ASATQQLNDVVDAIINVSLIDSESLKFALVPIELSALVEVIVRELQPLAKQRDQQILT
                     GDLANLPKVPADPNYLRRALTNLISNAIKYTPDGGIITLEAKQEHEAIHIVVSDTGIG
                     IDHSELTRIFEKFYVLEDTKNHSSSHNAFMGGGLGLGLTVVRGIIEAHGGRIWVESEG
                     EDPARMPGSRFHILLPLIAPKQ"
     gene            1560..2534
                     /locus_tag="VFF70_14990"
     CDS             1560..2534
                     /locus_tag="VFF70_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZY46042.1"
                     /translation="MPASANRKNGIRAAMLSALFLGTTPILGKFAYLAGLNPYSLTAS
                     RTLIAAGVMWLFYLAVKSNRKYIYIYPAGLMISFFAGALNGASSLLYYNGLTHIDAGV
                     SQMLYSLYPLFVVIFRRMDGQRISHWTLIRLVLALIAVALLVLPVNDSRIDFVGVGLM
                     LGAGLMYAWHLAVSQRVLYEMPAPTVTLYVLTSMTAVVWIAFLIHGPAEVPVAGFTPM
                     LLLAAVTIISRLGMFLGVKYLGSIQTALIGVTEILIAVLLAIGVLGEQLMLRQWIGAG
                     ILITSLILIVFEPGIGLPAFQDRFKKKGPAANPIVPGSPPPHLTTPSA"
     gene            complement(2771..3863)
                     /gene="prfB"
                     /locus_tag="VFF70_14995"
     CDS             complement(join(2771..3796,3798..3863))
                     /gene="prfB"
                     /locus_tag="VFF70_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_102136549.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZY46043.1"
                     /translation="MEQMKITLTELNAKIERIMERLDIANKAQRVAELEQRAGQNDFW
                     NDPVSSQATMRELIELRNLVEGWQSFDRRAQDALELADLGDDSLADELTREVDSIQTE
                     FEQRELQLMLAGPHDRGSALMAFHAGAGGVDAADWTSMLVRMYLRWAETRGFKAEVVD
                     QVDADEGGIKNATISITGEYAYGYLRSERGVHRLVRLSPFDAAHRRHTSFALIEVWPD
                     LGEGEEVEIKPQDIKIDTYRSAGAGGQNVQKNETAIRITHLPTGIVVTCQNERSQTQN
                     REMALKILRAKLYEQEEENRLKELQNLKGEHLKAEWGSQIRSYVLQPYQMVKDHRTDH
                     EVGNAQAVLDGRLDDFMEAYLRFRLAAIE"
     gene            complement(3974..4885)
                     /gene="ftsY"
                     /locus_tag="VFF70_15000"
     CDS             complement(3974..4885)
                     /gene="ftsY"
                     /locus_tag="VFF70_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZY46044.1"
                     /translation="MLDRVKSIREALSRTRQATFGRVATLLGATTITPELWDELEALL
                     LQADVGTETTLAVLTRAKDRVAQAGITRADGLKTILKSELRALIFDAPPLDLHIFKAV
                     RVVLIVGTNGSGKTTTIAKLAAWYKAYGRSVLLAAGDTFRAAAGDQLEVWADRVGVPV
                     IGGQPGSDPGAVVFDALQAARSREVNIVLVDTAGRLHTKFNLMQELKKVRNVITKAIP
                     GAPHETWLVLDGTTGQNALMQAQQFKAAVAVTGIIITKLDGTAKGGMVFAVSHELGLP
                     IRFIGTGETMIDITPFDADVFVDGLLE"
     gene            5016..>5396
                     /locus_tag="VFF70_15005"
     CDS             5016..>5396
                     /locus_tag="VFF70_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZY46045.1"
                     /translation="MTSVSIVIPAYNEQENVKAAVEKVSASAEQLKLDYEILLVNDGS
                     RDQTGEIAKTELASRIPNFRLVEHYPNRGYGGALKAGFATATKELIVFIPADNQFDFS
                     EVPLLLDQMTPETMIVSGRRVKRQD"
BASE COUNT         1223 a         1521 c         1359 g         1293 t
ORIGIN      
        1 acaaagccct aagtgaagtg ccagtgattg tggttacggc caaagagctc accgccatcg
       61 aacggcagcg tttagccggt aaagcaagat cattgttgca aaaaggttcg ttcaccgatc
      121 tcgacatcgt cgattattta accgaggcct tagcctagcc gagcatgagc gcctaattcg
      181 tcgctaacaa cgtacggcgt aaccaacgcg ctgccgcgcg ggatgactgt gtctaacatg
      241 tgatcgtaca catatccgtt tcgttagcca tgacaactaa taagcttgca ttgaaaattt
      301 tatacatcga agatgacgcc aacagccgcc gcttggtgca gcgcgttctg gaggtcgaag
      361 gctatgaagt gcacgtcgcc gagaacggtt tggccggcct tgaaatggcg cgtcggttgc
      421 gccctgccct ggtactgacc gatgttaaca tgggcggcat gaccgggcac gaagtcgcca
      481 cgcgcctcaa agaaatgccg gagactcggc acgcgcccgt gatcgcgatt acctcgaacg
      541 tgttgcagac cgatcgggag ttagcgttgg tcgcaggctg tgatgggtac atcaccaaac
      601 cgatcgatgt ggatgcgctc cccaggttga ttgaaagatt tttggcgggc gaacgcgaga
      661 gcctgcctga taatgtacgt ctgttgcgat tggaagaata cagtcatacg ctggttaatc
      721 gcttggaatc gaccgttgcg gaactgcaaa cagcaaacga cgagctgcac cgtatggaca
      781 aaatgaagaa agacttcgtt gttttatcgt ctcacgaact gcggacgccg actacgttga
      841 tctatggtta catcaacttg ctcaaaatgg aaactaaaga cttgcccttg ggcgatcgtc
      901 tgatggatat gatcaatcgc attgcaagcg ccacccagca gctgaatgat gttgtcgacg
      961 cgatcatcaa cgtctcattg atcgattctg aatcgttgaa attcgcgtta gtcccgatcg
     1021 agctcagtgc cctggttgaa gtgatcgtcc gggaattgca accgttagct aaacaacgcg
     1081 atcaacagat tctcactggc gatttggcca atctacctaa agttcccgcc gaccccaatt
     1141 atctgcgcag ggcactcacg aacctgatta gcaacgccat taaatatacg ccggatggcg
     1201 gcatcatcac gctggaagcc aaacaggaac acgaggcgat ccatattgtc gttagcgaca
     1261 ccggcattgg tattgatcat tccgaactta ctcgcatctt tgagaaattt tacgtgctgg
     1321 aagacacgaa aaaccactcc tccagtcaca atgctttcat gggtggcgga ctgggtctgg
     1381 gactcactgt cgtacgagga attatcgagg cacacggcgg gcgcatttgg gttgaaagcg
     1441 aaggggagga tccggcgcgc atgcccggca gccgattcca cattttattg cctttgatcg
     1501 cgcccaaaca ataagtccaa ggcgatacag cctaacattc gttatggatc ggtgagacca
     1561 tgcctgcatc tgccaataga aaaaatggca tacgcgcagc gatgctatcc gccctctttt
     1621 tgggaacgac cccgattctc ggcaagtttg cctatctggc tggcctgaat ccgtacagcc
     1681 tcaccgcttc ccgcaccttg atcgccgctg gcgtcatgtg gcttttctat ctcgccgtta
     1741 aatccaaccg caaatatatt tacatttatc ccgccggctt gatgatctcc ttcttcgccg
     1801 gcgcgttgaa tggtgcaagt tcgctcttat attacaacgg cctcactcac atcgatgccg
     1861 gtgtcagcca gatgctgtac agtttgtatc cgctcttcgt cgtgatcttt cgccgcatgg
     1921 atggtcaacg catttcacat tggacactga tccggttagt cttggccttg attgcggtag
     1981 ccctcttggt cttgccagtc aacgatagtc ggatagattt tgtgggcgtg ggcttgatgt
     2041 taggcgccgg gttgatgtat gcgtggcact tagccgtgag ccagcgcgtc ttgtacgaaa
     2101 tgcccgcgcc aaccgtcacc ctctacgtgc tcacctccat gactgccgtt gtctggatcg
     2161 cattcttgat ccatggacct gctgaagttc cggtcgctgg cttcaccccc atgttgctgc
     2221 tcgccgccgt caccatcatc tccaggctcg ggatgttctt gggcgttaaa tatttaggca
     2281 gcattcaaac cgccctgatc ggcgtcaccg aaatcttgat cgccgtgctc ctcgcgatcg
     2341 gggtattggg cgaacagttg atgttgcgcc aatggatcgg cgctgggata ttgatcacca
     2401 gtttgatctt gatcgtattc gaaccgggta tcggtctgcc tgcgtttcaa gatcgattca
     2461 aaaagaaagg gccggcagct aacccgattg tgcccggcag cccgccgcct cacctcacca
     2521 cgcccagtgc ttagaaaaca aaagaccgct ctctcgagcg gccttttgct gtgcgtcgta
     2581 gcacccctat tccttaaacg gtcggaggta gtgctacgac gctgcgtttg ctattacaca
     2641 tatgcaccac ctcctcctct cttaaaggat agcaagtaaa taatgggtgg ggattttctc
     2701 cccaaagatg tgctaattat ccgccgaatt ctgcggcttg tcaaatccaa cttcacaggg
     2761 tgcagcgtat ttattcaatc gccgccaagc gaaagcgcaa ataggcttcc atgaaatcgt
     2821 cgaggcggcc atccaacacg gcctgcgcat taccaacttc atgatcagta cgatggtctt
     2881 tgaccatttg atacggctgc agcacatacg atcggatctg gctgccccat tcggccttca
     2941 aatgctcgcc ctttaaattc tgcaattctt ttaagcgatt ctcttcttcc tgctcgtaca
     3001 gcttcgcgcg tagaatcttc aaagccatct ctcgattttg agtttggctg cgttcatttt
     3061 ggcaagtcac cacaatccca gtcggcagat gcgtgatccg gatcgcggtt tcattcttct
     3121 gcacattctg cccgcccgcg cccgccgatc gataagtatc gatcttgatg tcctgcggtt
     3181 tgatctcgac ttcttcgcct tcacccagat caggccacac ttcgatcagc gcaaatgagg
     3241 tatgccgacg atgtgccgca tcgaacggcg acaacctcac taagcgatgc acgccgcgct
     3301 cggatcgcaa atagccgtaa gcatattcgc cggtgatcga aatcgtcgca ttcttgatgc
     3361 cgccctcatc cgcatcaact tgatcgacaa cttcggcttt gaagccgcgc gtttcggccc
     3421 agcgcaaata catccgcaca agcatgctag tccaatcggc agcgtcaacg ccgcccgctc
     3481 cggcatggaa cgccatcagc gcactgccgc gatcgtgcgg accggctaac atcaattgaa
     3541 gttcacgctg ttcaaactca gtctgaattg aatcaacttc gcgggttagt tcgtccgcta
     3601 gagaatcatc gccgaggtca gccaactcca acgcatcctg cgcgcgccga tcaaagctct
     3661 gccagccttc gacgaggttg cgcagctcaa tgagttcacg catcgtagct tgcgatgaaa
     3721 cggggtcgtt ccagaaatca ttttgaccgg cgcgctgctc caactcggca acgcgctgcg
     3781 ctttgttagc gatgtcaaag acgctccatg atacgttcga tcttggcgtt gagttcggtg
     3841 agggttatct tcatttgttc cattacgtta ttccgatcct tcagctaatc tgccacaaag
     3901 acactaaggc accaagtttt cttcattttg ttttaccgtg tgtcttagtg gcttcgtggt
     3961 tattttacat ccattattca agcagtccat cgacgaacac atctgcatcg aacggtgtga
     4021 tatcgatcat tgtctcgccc gtgccaatga agcggatcgg caaaccgagt tcatgcgaga
     4081 ccgcaaagac catgccgccc ttggcggtgc catccaactt cgtaatgatg atgccggtca
     4141 cagccaccgc cgctttaaat tgctgcgctt gcatcaatgc attctggccc gtcgtgccat
     4201 ccagcaccaa ccatgtttca tgcggcgcac ccggaatcgc tttggtgatc acgttgcgca
     4261 ctttcttgag ttcctgcatc aaattaaatt tcgtatgcag gcgtccagcg gtatcgacca
     4321 gaacgatgtt gacttcgcgc gatcgagcag cttgcagcgc atcgaacacg accgcgcccg
     4381 gatcagagcc cggttgtccg ccgatgaccg gtacacccac ccgatcagcc cacacttcca
     4441 attgatcacc ggccgccgcg cgaaaggtat cgcccgcggc cagcaaaact gatcggccgt
     4501 acgctttata ccaagccgct aacttcgcaa tcgtcgtcgt cttgcccgaa ccgttcgtgc
     4561 cgacgatcaa tacgacccgc acagccttga agatatgcag atcgagcggc ggtgcatcga
     4621 agatcagcgc gcgcaattca gacttcagga tcgtcttcaa gccatcggcg cgcgtgatcc
     4681 ctgcctgcgc tacgcgatct ttagcgcggg tcagcaccgc cagcgttgtt tcggtgccga
     4741 catctgcttg aagcagcaag gcctccagtt cgtcccacaa ttcaggcgtg atggtggtcg
     4801 cgcctaataa agttgcgacg cggccgaacg tcgcttggcg cgtgcgggac aatgcctcgc
     4861 ggatcgattt aacccgatcg agcataacct aatctccagt gaagcgcggc gattatacct
     4921 gatcgatcag ggatcggcaa cgcgcgcagc gcgctttagc gatcgatcgg tatgttataa
     4981 tcacgctgac tgattcagaa ataaaaggcg cgttcatgac gagtgtatcg attgtaatac
     5041 cggcctataa cgagcaggag aatgttaaag cggccgttga aaaagtttcg gcgagtgcgg
     5101 aacaactcaa actggattat gaaatcttat tagtcaatga tggcagccgc gatcaaaccg
     5161 gcgagatcgc caagacagag ttggcgtcgc ggatccccaa ctttcgctta gtagaacact
     5221 atcccaatcg gggttacggt ggcgctttga aagccggttt cgccaccgcc accaaggagc
     5281 tgatcgtttt cattcccgcc gacaatcaat ttgactttag cgaagtgccg ttgctcttgg
     5341 accaaatgac gccggagacg atgatcgtca gcggacggcg cgtcaagcgt caagac
//
LOCUS       DASRTJ010000743         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_217323,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000743 DASRTJ010000000
VERSION     DASRTJ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217323"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFF70_15010"
     CDS             <1..500
                     /locus_tag="VFF70_15010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZY46046.1"
                     /translation="VVAADRVLATVLFLDIVGSTERAVAIGDASWHTLLDRYYETVRK
                     ELARYRGKEIDTAGDGFFATFDGPARAIRCACAIRDSVKPLGIEVRSGLHTGECELIG
                     EKVGGIAVHIGARVMSKAGASEVLVSSTIKDLVAGSGIEFEDRGLHALKGIPSEWRLS
                     AISHT"
     gene            516..821
                     /gene="paaB"
                     /locus_tag="VFF70_15015"
     CDS             516..821
                     /gene="paaB"
                     /locus_tag="VFF70_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HZY46047.1"
                     /translation="MTDTQWKTFEVFHQSKRGEPHVHVGAVHATDPEMALLLARDQFA
                     RRLDCVNLWVVPSDQIFATNYEDSEALFEPATDKSYREASGFPLRDAIKKAENWKEE"
     gene            973..1713
                     /gene="paaC"
                     /locus_tag="VFF70_15020"
     CDS             973..1713
                     /gene="paaC"
                     /locus_tag="VFF70_15020"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HZY46048.1"
                     /translation="MDHTALIALLTSLADDELILGHRNSEWTGHAPIIEEDIAFSNIA
                     QDEMGHALLWYTILNEQLGQPLPDRMAFFRSADQFRNAQFVELPKGDWAITIVRQYLF
                     DVYEQVRNAELIHSDFQPIIDVVSKIKREEVYHLMHSKGWLLRLGDATEESHTRMQTA
                     LDQLWPHALGLFEPIEEAPEYSAKLNDLRQSWLDIVTPILTQATLIIPANVDPIFGGR
                     KHQHTPHLIDLLKDMQKVARMESPETVW"
     gene            1717..>2778
                     /locus_tag="VFF70_15025"
     CDS             1717..>2778
                     /locus_tag="VFF70_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46049.1"
                     /translation="MNIQIQTNASDPIQPGMIEPKLYPPSFLDRFMKAVERLPVPYWL
                     TYLALFILESAIFHILSWIDGWLPAYTVNPLLLLFPLWLWGPLAIITYLNSISLGALA
                     SFAPLLNIQEESMQRLKNEFTTMPTRSVIISGVMWAIVYFIFTWVALQTFYGDYKIGT
                     LLTVLIIIEGLVSYSTGSIIYYHSIRQLILVNRTLQLVKRFNLFQLDPVYAFSLVTAR
                     TGMAWMILLSLTLLTFPIQLAPIPILVLLSLQLVLAISAFVLPLQIVHHRLVLEKRRL
                     WAEVNQRVESATAQLHQRLDENDLSKMIELDHAIMGLNAERDILAKISTWPWRSGLLT
                     GFLSITILPIILFLVQLALG"
BASE COUNT          638 a          752 c          732 g          656 t
ORIGIN      
        1 cggtggtcgc agccgatcgg gtgttggcga cggttttgtt tttggatatc gtcggctcaa
       61 ctgaacgcgc cgtggctata ggcgatgcaa gttggcacac gctgctcgat cgatattacg
      121 aaacagtacg caaagaactg gcgcgctatc gcggcaaaga gatcgataca gcgggcgatg
      181 gattcttcgc gacattcgac gggcctgccc gcgcgatccg ctgcgcctgc gcgatccgcg
      241 actccgtaaa accgttgggc atcgaagttc gatcgggttt gcacacgggc gaatgcgaat
      301 tgatcggcga aaaagtgggc ggcatcgcgg tgcacatcgg cgcgcgcgtc atgtcgaaag
      361 ccggagcgag tgaggtttta gtgtcgagta cgatcaaaga tttggtcgcg ggctcaggga
      421 ttgaatttga agatcgcggt ctgcatgcgc ttaaaggcat tccgagcgaa tggcgcttgt
      481 cggcaatcag ccacacttaa acggatgatg aatcaatgac cgatactcaa tggaagacat
      541 tcgaagtgtt tcatcaaagc aagcgcggcg agccgcatgt ccacgtcggc gcagtgcatg
      601 ccaccgatcc ggagatggcg ctgctactgg ctcgcgatca atttgcgcgc cgattggatt
      661 gcgtcaactt atgggtcgtg ccaagcgatc agatctttgc cacaaactac gaagattccg
      721 aggcgttgtt cgaaccggcg accgataaga gttatcgaga ggccagcggc ttcccgctgc
      781 gcgatgcgat caagaaagcg gagaattgga aagaagaata attttcacgc gtcattgcga
      841 gcaacacttg caccgcaatg tcctgcgatg gtgcatccgc aggatgtacc acggagcagt
      901 gcaggtgtct cattatcgat tttgagattg cttcttcgcc tacggggctc gcaatgacgg
      961 ccaaaggcaa ttatggacca caccgcactc atcgctttgc tcacttctct tgccgacgac
     1021 gaattgatcc ttggtcatcg caactccgaa tggaccggcc acgcgccgat catcgaagag
     1081 gacattgctt tctcgaacat cgcgcaggat gagatggggc atgcgctgtt gtggtacacc
     1141 atcctcaacg aacaactcgg ccagcctctc cccgatcgaa tggcattttt tcgaagcgcc
     1201 gatcaatttc gcaacgcgca gttcgtcgaa ctccctaaag gcgattgggc gatcacaatc
     1261 gtgcggcagt acctgttcga tgtctacgag caagtccgca atgccgaatt gattcacagc
     1321 gattttcaac ctatcatcga tgtcgtgagt aagatcaagc gagaggaagt gtatcacctg
     1381 atgcacagca aaggctggtt gctgcggctc ggcgacgcga ccgaagaaag ccacacccga
     1441 atgcaaaccg cgctcgatca attgtggccg cacgcgctcg gcttgttcga gccgattgaa
     1501 gaagcgccag agtattcagc caagttaaat gatctgcgtc aaagctggct ggacatcgtc
     1561 acaccgattt tgactcaagc cacattgatc atccccgcca acgtcgatcc gatcttcggg
     1621 ggacggaagc accagcacac gccgcatttg atcgatctat tgaaggacat gcagaaggtc
     1681 gcgcgaatgg aatcgccgga gacggtatgg tgaaacatga atattcaaat ccaaactaac
     1741 gcatctgatc cgatccaacc gggaatgatc gaacccaaac tctatccgcc gagttttctc
     1801 gatcggttca tgaaggctgt cgagcggctg ccggtcccgt attggctgac ctatttggcg
     1861 ctctttattc tggaaagcgc gattttccac atcttgtcct ggattgatgg ctggctgccc
     1921 gcgtatacgg tcaatccact tcttcttctc ttcccactgt ggctatgggg accgctcgcg
     1981 atcatcactt atttgaactc gatctcgttg ggagccttag ccagcttcgc ccctctcctc
     2041 aacattcaag aggagtcgat gcaacggctc aaaaatgaat tcacgaccat gccgacccga
     2101 agtgtgatca tcagcggcgt catgtgggcg atcgtctatt tcatctttac gtgggtggcc
     2161 ctccaaactt tctatgggga ctacaaaatt ggaactctcc tcacagtgct tatcattatc
     2221 gaaggactgg tcagttattc cactggcagc atcatttact atcattcgat tcggcagtta
     2281 attctggtga atcgcacgct ccagctggtc aaacggttta acttgttcca actcgatccg
     2341 gtctacgcct tctcgctggt gacagcccgc acgggcatgg cctggatgat cctgctcagt
     2401 ctcactttac tcacctttcc aattcaactc gcgccgatcc ccattctggt cctgctgagt
     2461 ttgcaactcg tcttggcgat atccgctttt gtcctccccc ttcagattgt gcatcatcgg
     2521 ctggtcttag agaaacgccg actgtgggca gaagttaatc agcgggtcga atcagccacg
     2581 gcacagctgc accagcgtct ggatgaaaat gatttgagca aaatgataga actcgatcac
     2641 gctatcatgg ggctgaacgc tgaacgggac atcctggcga aaatttctac ctggccgtgg
     2701 cggtcaggct tattgaccgg ctttctatcc atcacgatat tgcccatcat cctttttctg
     2761 gtacaacttg cactgggg
//
LOCUS       DASRTJ010000744         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_217463,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000744 DASRTJ010000000
VERSION     DASRTJ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217463"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFF70_15030"
     CDS             complement(<1..142)
                     /locus_tag="VFF70_15030"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HZY46050.1"
                     /translation="MRIIARRALREFWEKQPDARQALQAWYADVKRAEWKTPAEIKAI
                     YRN"
     gene            342..611
                     /locus_tag="VFF70_15035"
     CDS             342..611
                     /locus_tag="VFF70_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZY46051.1"
                     /translation="MIIHITQVKVVGLHSLELTFDNGAHKRVNLSHELYGAVFEPLRD
                     PAYFARAYLDLDSRTVAWPNGADFAPDFLYNLEPETSPFVESTSL"
     gene            complement(714..>2065)
                     /locus_tag="VFF70_15040"
     CDS             complement(714..>2065)
                     /locus_tag="VFF70_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46052.1"
                     /translation="PHAGGYGVVGIGSDAPGVSVDNPGSGALIFGHELTHDYNVYHTN
                     TADACGSNDNNSDFPYPNSSIQEFGFNPDTGKVYNPSLTHDLMSYCPSGGSKQGWISP
                     FTWNKMYGKVPLTPSTASVDRGNNNLLPFTFYPATGGEALVVHTTISNPISNTNQIGG
                     YLGDMNRIEPALTYLPAPGDYSIELHNGPSILLSQTFTVSFQSEYSAHDPGDPGPTAV
                     DDVAFIMPWAEGTTSITLVVSGTVLDSRAVSLYSPVVTITDPTQVVTWTGGSHLLTWN
                     ASDADGDPLTYAVLFSHDGGSNWQLLDTGLISTSYNIDAGAMAGGNDVRFRVVASDGV
                     NIGYDETAYPIVIPNKPPMPMITNPISDTLFAPGDLIVFEGMAIDLEDGSLPDGSLDW
                     SSNRQGGVGLGPTVPMTGLLRGPQLITLTAMDSNGLTATTTVSIYIGYRAYLPIVRR"
BASE COUNT          457 a          572 c          545 g          491 t
ORIGIN      
        1 cattacgata aatagcctta atctcggcgg gtgttttcca ttcggcgcgt ttgacatccg
       61 cataccaggc ttgcaatgcc tgtcgagcat ccggctgttt ctcccaaaac tcacgcagtg
      121 cccgacgggc aatgatccgc atgtctacat tataccttgc tcccgatacg ggagcaaatt
      181 gtgctgccgc gctcgactat catcgcgagc atgtctatcg tgcgtgagtc ttccttccgt
      241 ttccattcgt gctgaagtgc cctctacagc gggttcgtga atcaatcttc cctgccgcgc
      301 aatttgggtt gtgatataat ttttgcacgg taacaaacaa catgatcatt catatcaccc
      361 aagtaaaagt ggtcgggtta cattcattag aattgacctt cgataacggc gcacacaaac
      421 gcgttaatct gagtcatgaa ctctatggcg ctgtcttcga accgctgcgc gatcccgcct
      481 actttgcgcg tgcttatctc gatctggatt cccgcacagt ggcctggccg aatggcgccg
      541 actttgcgcc tgatttcttg tacaatcttg aacccgaaac ctctccgttc gttgaatcga
      601 cttcgctgta gaagaagcgt atacttcgcc gcgcccgatg atcaccgtca gcatatctat
      661 cagccttaag acgctgcaca acgtcgaagg atcgcacgtg taaagcgatt caatcatctc
      721 cgcacaatcg gcaagtatgc tcgatagccg atgtagatcg atacggtagt ggttgcggtc
      781 aacccgttgc tatccatcgc cgttagcgtg atcaactgcg ggcctctcaa taaacccgtc
      841 attggaaccg ttggacccag acccaccccg ccttgacgat tgcttgacca atcgagcgat
      901 ccgtccggca gcgaaccatc ctccagatcg atcgccatac cttcgaagac gatgagatcg
      961 cccggtgcaa agagcgtgtc agagattgga ttggtgatca tcggcattgg cggcttgttt
     1021 ggaataacga tcggataagc ggtctcgtcg taaccaatgt tgacgccatc gctggctacc
     1081 acgcggaagc gcacatcatt gccgccggcc atcgcaccgg catcgatgtt gtacgacgtc
     1141 gaaatgaggc cggtatcgag cagctgccaa ttcgatccgc catcgtgact gaacaacact
     1201 gcgtaagtca acggatcacc gtcggcatcg ctggcgttcc acgtcaacaa gtgactgccg
     1261 cctgtccagg tcaccacctg cgttggatcc gtgatcgtca cgactggtga atacaaactc
     1321 actgcgcgcg aatcgagcac cgtgccgctg accaccaaag tgatcgaggt ggtgccctcg
     1381 gcccagggca tgatgaacgc cacatcgtcg acggctgttg ggccgggatc acccggatcg
     1441 tgcgcactgt attcgctctg aaagctgacg gtgaaagtct ggctcagcaa tatgctcggc
     1501 ccgttatgca gttcgatcga ataatcgccg ggcgcgggca ggtatgtcag cgccggttcg
     1561 attcggttca tatcgcccag ataaccgccg atttgattgg tgttgctgat cggattgctg
     1621 atcgtggtgt gtacgacgag ggcttcacca ccggtcgctg gatagaaagt gaaaggcagt
     1681 aaattgttat ttccccgatc gactgaagcg gtcgaaggtg tgagaggaac tttgccatac
     1741 attttgttcc aggtgaacgg cgagatccag ccctgctttg atccgccgga tggacagtag
     1801 ctcatcaagt catgggttag agacggatta tagactttgc ccgtatccgg attgaagccg
     1861 aattcttgaa tgcttgaatt cgggtaaggg aagtcagaat tgttgtcgtt cgagccgcag
     1921 gcatccgcgg tattggtgtg atagacattg taatcgtgtg tcagttcgtg cccgaagatc
     1981 agcgcgcccg aacccggatt atcgacgctg acgccgggcg catcgctgcc aatgccgaca
     2041 accccataac cgccggcatg cggat
//
LOCUS       DASRTJ010000745         1992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_217777,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000745 DASRTJ010000000
VERSION     DASRTJ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217777"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFF70_15045"
     CDS             <1..653
                     /locus_tag="VFF70_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46053.1"
                     /translation="KEYHYNDAKPGKPLYLLIPGGLIALAAIGLIIYIAYQTWFNHEL
                     ETSQGLPLILLLAPFYIGGVYLFSYGYELYDQKKALRLTAIIVFITVSFVVIIAVLFA
                     LAKGGSSSSKSSSSSSESSSSSSSGAGHSAAPAVSTTTRSGGSIIDPIFFVGGGGTHT
                     VTETREVIKEVPMAPKPINCPSCGRSYVPAENKFACPNCGTATPQDLIDQSQTPSA"
     gene            681..1487
                     /locus_tag="VFF70_15050"
     CDS             681..1487
                     /locus_tag="VFF70_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZY46054.1"
                     /translation="MSKIVDQHYLLHDQYRNSSNLDARISLHKRFSTNMYGWDRWCFD
                     LFQIQTTARLLELGCGPASLWLENRSRIPQDWDIALSDLSAGMIDRARENLQEVDRSF
                     QFEVIDAQSIPFDDAHFDAVIARHMLYHVPDRSKALQEIRRVLKPQGRFYCTTNGHTH
                     LRELHDLMRRFDPTTDFGWGKTSAEIFSLADGAELAPYFSEITVQRYADDLIVTEVEP
                     LVNYIFSMTTAAGLIDRRRAELTRFIQNEMAKTGSIHITKDTGMFIPTRT"
     gene            1652..>1992
                     /locus_tag="VFF70_15055"
     CDS             1652..>1992
                     /locus_tag="VFF70_15055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZY46055.1"
                     /translation="MTTPSRRVIDSWVWLLGLYSAASFIEAAFWGQMGSFTPLYLPKL
                     GIAPADVKSWTGIIVAISGLVGLPFLPLWGALADRYSRQPIIVRSFVAHLIAGIISIL
                     AGNVWVFLLGRS"
BASE COUNT          445 a          553 c          506 g          488 t
ORIGIN      
        1 tcaaagagta tcactataat gatgccaaac ccggcaaacc gctatacctt ttgatccccg
       61 gcggcttgat cgcactcgcc gcgattggct tgatcatcta catcgcttat cagacctggt
      121 tcaatcatga gctggaaacc agtcagggct tgccgctgat cttattgctc gcgccattct
      181 atatcggcgg cgtgtatttg ttcagttacg gttacgaact gtatgaccag aaaaaggctc
      241 tacgcttaac cgccatcatc gtattcatca ccgtctcgtt cgtcgtgatc atcgcggtac
      301 tgtttgcctt ggcgaaaggc ggctcatcga gctccaaatc ttcttcctcg tcttcagaat
      361 catcttcgtc gtcttcgagt ggcgcagggc atagcgcggc accagccgta tcaacgacca
      421 cgcgcagcgg cggcagcatc atcgatccga ttttctttgt cggcggtggc ggaacacaca
      481 cggttaccga aacgcgcgaa gtgatcaagg aagtaccgat ggcgccgaaa ccgatcaact
      541 gcccgtcatg cggccgatcg tacgtgccgg ccgaaaataa attcgcctgc ccgaattgcg
      601 gcacggccac gccgcaggat ttgatcgatc agagtcaaac cccgtctgcg taagggcgag
      661 gtgacctcgc ccctaccaac atgtccaaaa tcgtcgatca acactatctc ctccacgatc
      721 aataccgcaa ctcgtcgaac cttgatgccc gcatctcgct tcataaacgc ttcagcacga
      781 atatgtacgg ctgggatcgc tggtgtttcg atctattcca aattcaaacg accgctcgcc
      841 tgcttgaact cggctgcggt cccgcttcgc tatggcttga aaatcgatcg cgcattccac
      901 aagattggga tattgcgctg tctgatcttt cagccgggat gatcgatcgg gcgcgggaaa
      961 atctccaaga ggttgatcga tcgtttcagt ttgaagtgat cgatgcccaa tcgatcccct
     1021 tcgacgacgc gcatttcgat gcggtgatcg ccaggcacat gctgtatcat gtacccgatc
     1081 gatcaaaggc gctgcaagaa attcggcgtg tgcttaaacc gcagggtcga ttttactgca
     1141 cgactaacgg ccacacacat ctccgtgagt tacatgatct catgcgccgc ttcgatccca
     1201 cgaccgattt tggttggggc aagacttcgg ccgaaatatt ttcactggca gacggcgcag
     1261 aacttgcgcc atatttttct gaaatcactg ttcagcgcta tgcagacgat ttgatcgtga
     1321 ctgaggttga gccgctggtc aattacattt tttcgatgac gaccgctgca ggtttgatcg
     1381 atcgacgtcg cgccgagtta acgcgcttca tccaaaatga gatggcgaag actggatcga
     1441 tccacatcac gaaagatact gggatgttta taccaactcg gacttagcag tattgtcaca
     1501 cccgcggagg cgggtgtcca gcaatttcat tctggattcc cgctttcgcg ggaatgacta
     1561 ggaacatacg ctgcaaagaa caaatagaat tttcacaaca ccatttgaat caggtaataa
     1621 ctagaagact aacggcttaa tgagagagag catgactacc ccctcgcgac gcgtaatcga
     1681 ttcatgggtt tggctgctgg gtctgtattc ggcggcgagt ttcatcgaag cggccttctg
     1741 gggacagatg ggcagcttca cgccgttgta tctgcccaaa ctcggcatcg cgcccgccga
     1801 tgtcaaatcg tggacaggca tcatcgtcgc catctccgga ctcgtcggac tgccgtttct
     1861 gccgctctgg ggtgcgttag ccgatcggta ttcgcgccaa ccgatcatcg tgcgatcgtt
     1921 cgtcgcgcat ttgatcgccg ggatcatttc gatcttggct ggcaatgtgt gggtgttctt
     1981 gctgggccga tc
//
LOCUS       DASRTJ010000746         5233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_217982,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000746 DASRTJ010000000
VERSION     DASRTJ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217982"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VFF70_15060"
     CDS             complement(<1..107)
                     /locus_tag="VFF70_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZY46056.1"
                     /translation="MEQLIRDAGYAGLVAIIFAESGLFFGFFLPGDSLLL"
     gene            complement(222..968)
                     /gene="surE"
                     /locus_tag="VFF70_15065"
     CDS             complement(222..968)
                     /gene="surE"
                     /locus_tag="VFF70_15065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZY46057.1"
                     /translation="MNILLTNDDGYAASGLLILKQALDKLGTVKVVAPDQNWSAGGHV
                     KTMHKPLRLNEIQLADGTSAYASSGAPSDCIALVLLGAIDFKPDLIVSGINPNPNLGQ
                     DVTYSGTVTAAMEGTLGGIPSIAVSVESNEAYASAAEFVGQLIPIVLQKKLPPNILLN
                     VNVPSGSIKGVQVTRLGQRVYNDELVTRLDPRGKPYYWIGGEAPSGVPDDGTDLGALH
                     NGFISITPIQLDMTAIKFLDELTNWPLPDR"
     gene            complement(1218..4178)
                     /locus_tag="VFF70_15070"
     CDS             complement(1218..4178)
                     /locus_tag="VFF70_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZY46058.1"
                     /translation="MNTTDQRVKIIVGRRQRRQNQRGKGQKFIRIIGLALVAIVLSCG
                     VFFGSTVGGAVALYLNFTADLPDPSQLAAKFTQQNTEFFETTKIYDRTGKNLLYEVID
                     PRAGDRQWVSLDTIPELCRQAAIAIEDRSFYDNPGFDIRGMARAFLSNAQGGSVQGGS
                     SITQQLVKNTLIDPSQRTIQTGIEGYTRKLKEVLLSAEIGRRYPKDQILEWYLNTNFY
                     GNLAYGIQAASKIYFNKTVDRLNIAECAMLAAIPEFPSQNPIDNPADATSRQALVLDA
                     MLRDGYITADEAVAAKAEKLNVITGGVADRFDIVAPHFSVYVRKWLEDKFGADAVFRG
                     GLKVYTTLDLDLQNHAQQTVADQVKKLTEEDHNANNGAAVVIRPDTGEILAMVGSADY
                     WNDAINGKFNVAIGARQPGSSFKPFTYITLLSQGIPASFSFLDVRTEFQQPGANPPIY
                     VPENYDRKYHGYTRLRLALARSYNIPAVLSLERAGVDNVIRTAHKMGITTLDRGLDYY
                     GLALTLGGGEVKLLDETYAFGVFGNNGVMAGSPVPEDRQRPGFRTLDPVPVLRVEDSK
                     GNIVWEYSTPASAQILDPRLSYLMNSILSDKGARCAAFGCPNILELDDNRPTAVKTGT
                     TNDFKDNWTVGYTPQVVVGVWIGNTDNTSMKHVTGISGAAPVWHDIISYYLKDKPKVP
                     FVRPPGLVEKTICATTGLLPTKYCPKTTEIFIPGTEPTSYDTLDQAFLIDKETGNLAT
                     ASTPPDKVEEKIFEIYPPEAADYVKEANIPQPPAQYDTNYGPPPNAYGDVAIIGPGPY
                     TYIHGIYPLTGNAKGDDWNHYRIDFGKGLNPTEWQQIGPDHGEQVDQGPLENWDTSAL
                     DGLFSLRLTAFRNNGDTQISVLPVTVDNISPTIKIIYPNSGDIYEYGQDEWVNIQLDV
                     QDNIAIGTVQLFVDDKPEPWSTHRAPPYGDKWLLDSGGKLGTHTFFARVYDKAGNVKD
                     SNKVTVKIVPKKTP"
     gene            complement(4290..5024)
                     /locus_tag="VFF70_15075"
     CDS             complement(4290..5024)
                     /locus_tag="VFF70_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZY46059.1"
                     /translation="MTHQPSARAALRGEPSYVWRFGQNRRLDMILHALPIDARRILVD
                     GCGLGLYVKHLSVLGYNTIGLDVDFDRVVEGTRAGIEQLHVAAGEHLPYPDRSFDAIL
                     SHEVIEHVDNDRQAAQEMIRVLRPGGRVILFCPNRWYPFETHGHYWHGKYQFGNTPLI
                     NYLPNPIRNRLAPHVRAYTGHALRNLFRGTPSRVITHTQIYPGYDNLVKRRPTIGKVL
                     RAITYTFERTPLKFFGLSHFLVVEKT"
     gene            complement(5021..>5233)
                     /locus_tag="VFF70_15080"
     CDS             complement(5021..>5233)
                     /locus_tag="VFF70_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46060.1"
                     /translation="QGVQIDGQARRLIDPDETDRAWALFRDKFPFTTEFVDQIARSSF
                     YTLTPKWIRLIDNSKEFGHREEFTLP"
BASE COUNT         1185 a         1368 c         1438 g         1242 t
ORIGIN      
        1 agcaacaagc tatcgcccgg taagaagaag ccgaagaaca aacccgactc ggcaaagatg
       61 atggccacca ggccggcata gcctgcatct ctaattaatt gttccatatt actttgttcc
      121 tttttttgtg gcttaaaaca agaccccgca aataccagcg gggtctatct tgtataacgc
      181 gccagagaat accacatttc ccagctcgcg acaaggcaag ctcagcgatc gggtaacggc
      241 cagtttgtta attcatccaa aaacttgatc gcggtcatat cgagttgaat cggggtgatc
      301 gagatgaagc cgttgtgcag cgcacccaga tcggtgccat catccggcac gccgctcggc
      361 gcttcaccgc cgatccagta atacggtttg ccgcgcggat cgagccgggt cacgagttca
      421 tcattgtaga cgcgctggcc caagcgcgtg acctgcaccc ctttaatcga tccgcttggc
      481 acgttgacgt tgagcaggat attcggcgga agttttttct gcagcacgat gggtatgagc
      541 tgcccgacga actccgccgc ggacgcatac gcttcatttg attcaaccga cacagcgatc
      601 gacgggatgc cgcctaacgt gccttccatg gcggcagtta ctgttccaga ataagtcaca
      661 tcttgcccta gattcggatt ggggttgatc ccagacacga tcagatcagg tttgaaatcg
      721 atcgcgccga gcaagaccag cgctatgcag tcgctgggcg cgccgctgct tgcataggct
      781 gaggttccat ccgctaattg aatttcgttt agccgcagcg gcttgtgcat cgttttgaca
      841 tgaccgccgg cgctccagtt ttgatcgggc gccacgactt tgaccgtgcc aagtttatcg
      901 agcgcttgtt tcaagatcaa caggccggac gcggcgtagc cgtcgtcgtt ggtgagtaga
      961 atgttcattg aaagttctcc tgaaaaggga taagtgaaac gtgatgtgtc ttgacgcgat
     1021 gtgcaacttt ttcttgcaga aggaaaggtt gccgccgtcg ttgcgagcga agcgaagcaa
     1081 tctccacgac actcagctga gattgcttcg gcgcaaaaag acgcgcctca caatgacggc
     1141 tttaaagagt ggcaacgaat agcccgctct tggagtgtat caagaacggg ctaaacatgc
     1201 tgattatacc tctgccgtta aggagtcttc ttcggaacga tcttaacggt aactttattg
     1261 ctgtccttga cattgccggc tttgtcatag acgcgtgcaa aaaaagtgtg cgtgccgagc
     1321 ttacctccag aatcgagcag ccatttatca ccatagggcg gagcacggtg tgttgaccac
     1381 ggctcaggct tgtcgtcgac gaacaactgc acagtgccga tggcaatatt atcctgcacg
     1441 tcgagttgaa tgttcaccca ttcatcttgt ccgtattcgt aaatgtcgcc gctgttcgga
     1501 tagataatct tgatcgtcgg tgaaatgtta tcgacggtca cgggcaacac cgaaatctgt
     1561 gtatcgccgt tgttgcggaa cgcagtcagg cggaggctga ataaaccatc gagggcagag
     1621 gtatcccaat tttccagcgg accttgatcg acttgctcgc cgtgatcggg cccaatctgc
     1681 tgccactcag taggattcaa gcctttgcca aaatcgatcc gataatgatt ccagtcgtcg
     1741 cctttggcgt tgccggtaag cggatagatg ccgtggatgt aggtatacgg ccccggcccg
     1801 atgatcgcca catcgccata cgcattgggt ggcgggccgt agttggtgtc atattgtgcc
     1861 ggtggctgtg gaatgttggc ttctttaaca tagtcagccg cttccggcgg atagatctca
     1921 aatatttttt cttcgacctt atcgggcggg gtggaggccg tagccaagtt gcccgtttct
     1981 ttatcgatca agaaagcttg atcgagcgtg tcgtaagaag taggctcagt gcccggaatg
     2041 aagatctccg ttgtcttcgg acaatattta gtcggcagta accccgtggt ggcgcagatt
     2101 gttttctcaa ccaaccccgg cggtcgaacg aacgggactt tgggtttgtc tttcagataa
     2161 tacgaaatga tgtcatgcca caccggcgcc gcgccagaaa ttccggtcac gtgcttcatc
     2221 gaagtattat cggtgttgcc gatccacacc ccgacgacga cttgcggtgt ataaccgacc
     2281 gtccagttgt ctttgaagtc gtttgttgtg cccgtcttaa cagcagtcgg tcgattatcg
     2341 tcgagttcca aaatgttcgg gcaaccaaac gccgcgcagc gcgctccttt atccgataag
     2401 atactattca tcaaataaga caaccgcggg tctaatatct gcgcagaggc aggtgtactg
     2461 tattcccata cgatattgcc tttgctgtct tcgacgcgca acaccggcac tggatcgagc
     2521 gtgcgaaagc cggggcgttg tcgatcttcc ggaacgggcg agccggccat gacgccattg
     2581 ttgccaaaca cgccaaacgc ataagtttca tcgagcagct tcacttcgcc gccgcccaaa
     2641 gttaaagcga gtccataata atccaagccg cgatcgagtg tggtgatgcc catcttgtgt
     2701 gcggtgcgga tcacattatc aacgccggcc ctttccagcg aaagcacggc ggggatgttg
     2761 tacgatcggg ctaaagctaa ccgcaaccgt gtgtagccgt gatacttgcg atcgtaattc
     2821 tccggcacat agatcggcgg attcgcgccc ggctgttgaa attcggtgcg cacatccaga
     2881 aatgaaaagg acgccggaat gccttgcgac aacagcgtga tataagtgaa gggcttgaac
     2941 gacgagccgg gctgccgggc gccgatggcg acattgaact tgccattgat cgcgtcattc
     3001 caataatccg cgctgccaac catcgccaag atttcaccgg tatctgggcg gatcacgacg
     3061 gcggcgccat tgttggcgtt gtgatcttct tcggtaagtt tcttaacttg atcggcgacg
     3121 gtctgttgag cgtgattctg tagatcgaga tcgagtgtgg tgtagacttt tagcccgccg
     3181 cgaaagaccg catccgcgcc gaatttatct tccagccatt tacgaacgta caccgagaaa
     3241 tgtggcgcga cgatatcgaa gcggtcggcc acaccgccgg tgatgacatt taatttttca
     3301 gctttcgcgg caaccgcttc atcggctgtg atatagccgt cgcgcagcat cgcatccagc
     3361 actaaggctt gacgcgaggt cgcatcggcc ggattatcga tcgggttctg gctgggaaac
     3421 tctggaatcg cggccaacat tgcacattcg gcgatattca gccgatcgac cgttttgttg
     3481 aaatagattt ttgaagcggc ctgaatgccg taagccaggt tgccgtagaa gttcgtgttg
     3541 agataccatt ctaaaatttg gtctttcggg tagcgccgac cgatttcggc ggagagcaaa
     3601 acttctttga gcttgcgcgt ataaccttca atgccggttt gaatggtgcg ctgactcgga
     3661 tcgatcagcg tattcttcac cagctgttgg gtgatcgacg agccgccttg caccgatccg
     3721 ccttgcgcgt tgctgagaaa tgcgcgcgcc atgccgcgaa tatcgaagcc gggattgtcg
     3781 tagaagctgc gatcttcgat cgctatggca gcctggcggc acagttctgg aatcgtatcg
     3841 agcgacaccc actgccgatc gccggcgcgg ggatcgatta cttcatacaa cagattcttg
     3901 ccagtacgat cgtaaatctt cgtcgtctca aaaaattctg tattttgttg ggtgaacttc
     3961 gcggctaact ggctgggatc aggcagatcg gcggtgaagt tgagatagag cgccaccgcg
     4021 ccgccgaccg tcgagccgaa gaacacaccg cagcttaaca cgattgcgac aagcgcaaga
     4081 ccaatgatgc gaatgaactt ttggcctttg ccgcgctgat tctgtcggcg ctgccgccgg
     4141 ccgacgatta tttttacacg ctgatcagtt gtattcaaac ttacatacct tttatgcaaa
     4201 cgtcattgcg atcgcaatga cgctgcatga ttcaatctta accgcgaaca gattttcacg
     4261 caagaatgag ggaaaggggc ggtgtgaaat caggttttct caactaccag gaaatgcgat
     4321 aggccgaaga acttcagggg tgttcgttcg aaagtgtacg taatcgcgcg cagcactttc
     4381 ccgatggttg gtcggcgctt gacgaggttg tcgtagccgg gataaatttg agtatgcgtg
     4441 atgacgcgtg atggcgtacc gcgaaataga ttgcggagag cgtgtccggt atacgcacgc
     4501 acatgcggtg ccagtcgatt gcgaatggga ttcggcaagt agttaatgag cggggtgttg
     4561 ccgaattgat attttccatg ccagtaatgc ccgtgcgttt cgaacggata ccagcgattg
     4621 ggacaaaaca aaatgactcg accgcccggc cgcaacacgc ggatcatttc ttgcgccgcc
     4681 tgtcgatcgt tgtccacatg ctcaatcact tcgtgcgaca agatcgcgtc gaatgatcga
     4741 tctggatagg gcaagtgctc gcccgccgct acatgaagtt gctcaatgcc cgcgcgcgtt
     4801 ccttcgacga cgcgatcgaa atcgacgtcc agcccgatcg tgttgtagcc taacacgctg
     4861 agatgtttca cgtataagcc gagaccacag ccatcgacca aaatacgacg cgcatcgatc
     4921 ggcaaagcgt gcaggatcat atcgaggcgg cgattctgtc cgaagcgcca cacgtacgat
     4981 ggttcgccgc gcagggcggc tctcgctgaa ggttgatgcg tcacggtagc gtaaactctt
     5041 cgcgatggcc gaactctttg gagttgtcga tcaggcgaat ccactttggg gtgagagtgt
     5101 aaaacgaact gcgggcgatc tgatcgacga actcagtggt gaaagggaac ttgtcgcgga
     5161 agagcgccca cgcccgatcg gtttcatccg gatcgatcaa gcgccgcgcc tgtccatcga
     5221 tctgcacgcc ttg
//
LOCUS       DASRTJ010000747         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_219796,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000747 DASRTJ010000000
VERSION     DASRTJ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219796"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..367)
                     /locus_tag="VFF70_15085"
     CDS             complement(167..367)
                     /locus_tag="VFF70_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46061.1"
                     /translation="MKRLITPSIFIPIVIVLLIGISTANAQRTNDPASPQSGYDLSWY
                     TIDGGGATFSIGGSYSQMVKRH"
     gene            complement(364..1131)
                     /locus_tag="VFF70_15090"
     CDS             complement(364..1131)
                     /locus_tag="VFF70_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZY46062.1"
                     /translation="MNTASDTFATVGGGYGNTASGGNATVAGGQNNAANGLNSMILGG
                     TNNTVTGTNSMAGGYNALANGDGCFAWGDHSTSNVIDCQGNNLWSIRATGGFYFRPSS
                     TVLCSLIDASGWQCIPVSDRNAKQDFVSVNPRQVLNQVVGLPIEQWAYTDDATHIQHI
                     GPMAQDFYAAFNVGADDKHINPVDSIGVALAAIQGLYQENQDLKGQNASLETRLTNLE
                     QNAQPVPFNWINLLGVIAFAGFLSNWIQQRRTTRGQS"
     gene            complement(1128..1868)
                     /locus_tag="VFF70_15095"
     CDS             complement(1128..1868)
                     /locus_tag="VFF70_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46063.1"
                     /translation="MGNRIGITQTVSNVSVNRGLFTAQLNVGNQFGDTAFNGEARWLS
                     IAVRCPTGGTYTPLNPRQPLTSLPYALPGLRTFPNATSPNVVGGFSGHIILASVVGGV
                     IGGGGLSGFPNRVRAYYASIGGGYANIASGYEANVDGGASNTASGFAATVGGGSYITA
                     SGTIATVGGGQFNTASNAETTVGGGFYNTASGNAATVGGGYTTPPATLKPPSAGASTT
                     PPAVLPPPSAGANSTPPAALPPPSAGAT"
     gene            complement(1942..2118)
                     /locus_tag="VFF70_15100"
     CDS             complement(1942..2118)
                     /locus_tag="VFF70_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46064.1"
                     /translation="MKHNFVVGIIGGLIGSAMLLIVLSATGIVGARDADGSRSDVSHV
                     NSVAAATLTSTFTY"
     gene            complement(2378..2881)
                     /locus_tag="VFF70_15105"
     CDS             complement(2378..2881)
                     /locus_tag="VFF70_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZY46065.1"
                     /translation="MEFRSGDHAVHPTHGVGNILRREEKQLAGSASLPYYLLAVGTTI
                     VWVPIAADGSTSLRAVTTKRELEQYRKLLKSRPSTIEREHKQWRFDTNARLTHGTFKV
                     LCEVVRDLTSLGWYRTIGEIDSGMLQKVRNNLRREWAAAAGLSLQDAIQEVDALLIAG
                     RDEFKTA"
     gene            complement(2932..3216)
                     /locus_tag="VFF70_15110"
     CDS             complement(2932..3216)
                     /locus_tag="VFF70_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZY46066.1"
                     /translation="MEAKLYVGNLSYTTSEDELRTLFAQAGEVASVAVIKDRDSGTSK
                     GFGFVEMTSQVDAQKAITMFNEYKMGDRALLVNMAKPRVERSTNNRTNRF"
     gene            3506..>4195
                     /locus_tag="VFF70_15115"
     CDS             3506..>4195
                     /locus_tag="VFF70_15115"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin family protein"
                     /protein_id="HZY46067.1"
                     /translation="MDDQTNLRTKAQAGAQDSDRYFKYMAEFVGLSKGDINAIRQTAP
                     VIEHHLPEIVAKFYSHLLRYPPTRKFFLKNDGSIDQEYVELRMRHLTNFWLRAAQGNF
                     DDEFARYIDYVGRAHTSHGADPTIYIAERYVIGQVGFMQHAIAEAITRDLRHVNEDLE
                     TSAIEAWDRLMMVILEMLARAYGNEREAETFDALVTIDQQAVNRLAQEAFTFEHDKDK
                     PVAHKDIVVGRA"
BASE COUNT          923 a         1177 c         1151 g          944 t
ORIGIN      
        1 gttcgaaatg acaagcgaaa gtcctgttac gaattcttca acgccagtgg tagatagatt
       61 ttgtaagtaa ctgcctctcc tccccagaat ccaccattga gttgatacga tccgccgctc
      121 agcgatcccg catccggctg cccgatcgat ccgccgatgg tacgcgtcag tgacgcttta
      181 ccatttgcga atacgatccg ccgatgctga aggttgcgcc gccgccatcg atggtgtacc
      241 acgatagatc atagccgctt tgcggagacg cgggatcgtt cgtgcgttga gcgttcgccg
      301 tgctgatgcc gatcagcagc actataacga tcgggatgaa aatactcgga gtgattaggc
      361 gtttcatgac tgacctcgtg ttgttcgacg ctgctgaatc cagttggaca agaatccagc
      421 aaaggcgatc acgccaagca gattgatcca gttgaagggg actggttgag cgttctgctc
      481 aagatttgtc aatcgcgttt caagcgaagc gttctgccct ttcaagtctt gattctcttg
      541 atacaagcct tgaatcgctg cgagcgccac gccgatagaa tcgaccggat tgatgtgttt
      601 atcgtctgcg ccgacgttga acgcggcgta aaaatcctgc gccatcggtc cgatgtgttg
      661 aatatgtgtg gcatcatctg tgtaagccca ctgctcaatc ggcaagccaa caacctggtt
      721 caacacttgg cgcggattca cggaaacgaa gtcttgcttt gcgttgcggt cgctgactgg
      781 tatgcattgc caaccggacg cgtcgatcag agagcagagg acagtgctgc tagggcgaaa
      841 gtaaaagccg ccggtggcgc ggattgacca taaattattg ccctgacaat caatcacgtt
      901 gctcgtgctg tgatcgcccc acgcgaagca gccgtctccg ttggcgagcg cattgtaccc
      961 gcctgccata ctgttcgttc cagtgacagt attgttggtc ccgccgagaa tcatggagtt
     1021 cagaccattg gcggcgttgt tctggccccc ggcaacggtg gcgttaccgc cgctggcggt
     1081 gttgccgtag cccccgccga cagtggcgaa ggtgtcactg gcggtgttca cgtagccccc
     1141 gccgacggtg gcggcaaagc cgctggcggt gttgaattcg cccccgccga cggtggcggc
     1201 agaaccgctg gcggtgttgt agaagccccc gccgacggtg gtttcagcgt tgctggcggt
     1261 gttgtatagc ccccgccgac agtggctgca ttgccgctgg cagtgttgta gaagcccccg
     1321 ccgacggtgg tttcagcgtt gctggcggtg ttgaattggc ctccgccgac ggtggcaata
     1381 gtgccgctgg cggtgatgta ggagcccccg ccgacggtgg cggcaaagcc gctcgcggtg
     1441 ttgctggcac ccccgtcgac gttggcttcg tagccgctgg cgatgttggc atagcccccg
     1501 ccgatggaag cataatacgc ccgcacacga ttcggaaacc cactcaagcc gccaccgccg
     1561 atcacaccgc ccacgacgct ggccaagatg atatggccgc tgaagccgcc gacgacgttt
     1621 ggactggtcg cattcggaaa ggtgcgcaag ccgggcaagg catatggcaa cgaagtcaat
     1681 ggctgacgtg ggttgagcgg agtatatgtg cctccagttg gacaacgcac cgcgatcgac
     1741 aaccagcgcg cctcgccatt gaaagcggtg tcgccaaatt gattaccgac atttaactgc
     1801 gcagtgaaca agccacggtt cacactcacg ttgctcaccg tctgcgtgat gccgattcga
     1861 ttgcccaagc taagcgcatc atacaaactg aattgaaaat cgcacgtatt actaacaggc
     1921 gatccgctag accaattgtc cttaataggt gaacgtcgag gtcagtgtcg cagctgcgac
     1981 ggagttgacg tgcgacacat ctgatcgaga accatccgca tcacgagcgc cgacgatgcc
     2041 cgtggcgctc aacacgatca gcagcatggc cgatccgatc aaaccaccga taatcccaac
     2101 aacaaagttg tgtttcatgg cttgcctcct atggcagtga acgagtgctc agcggtctgc
     2161 cttgcgaagt ggcgcgtcga gcgattttag attgaatatc gaacgaacac tttttcgatc
     2221 acgaccccgc tggctctgaa gaatatcgag agtcctatcg acggtcgttt ctcctgctgt
     2281 atacttgcct agaaaacgca accgcctctc cacaattcta ttgaattgca gagaggcggt
     2341 ctttgatttt tatcttcagc gctgggcgcg ccgtggctca ggctgttttg aattcgtccc
     2401 ggccggcgat cagcagcgca tccacttctt ggatggcgtc ttgtaacgac aagcccgcgg
     2461 ccgctgccca ttcccgccgc aggttgttgc gaaccttctg cagcatgccc gaatcgatct
     2521 cgccgatcgt tcgataccag cccagcgacg tgagatcgcg caccacttcg cacaagactt
     2581 taaacgtgcc gtgggtcaga cgcgcattcg tatcgaagcg ccattgtttg tgttcgcgct
     2641 cgatcgtgct cgggcggctc ttcagcagct tgcgatactg ttctagttcc cgcttcgtcg
     2701 ttacggcgcg cagactcgtc gatccatcgg cagcgatcgg cacccagaca atcgtcgtgc
     2761 ccacggccag cagatagtat ggcagtgacg cggacccggc cagttgtttt tcttcgcgcc
     2821 gcaggatgtt acccacacca tgcgtgggat gcaccgcatg atcacccgat ctgaattcca
     2881 tgttgatgct ccttgtatgg tcgccagata cagattgcgg acagaaccag cttagaaccg
     2941 attggttcga ttattggtgc tgcgctcgac gcgcggcttg gccatattga ccagcagtgc
     3001 tcgatcgccc atcttgtatt cattgaacat cgtgatcgct ttttgcgcat ccacctgcga
     3061 tgtcatttcg acaaagccaa agcccttcga ggtgccggaa tcgcgatcct tgatcacggc
     3121 cacagatgcg acttcaccgg cttgtgcaaa cagcgtgcgc agctcgtctt ccgaagtcgt
     3181 gtacgacaga tttccaacat acaactttgc ttccattttt ccaatctcct tgattgaccg
     3241 gcacacagtt gtgacggcaa taaaaaaccg ccagatcaca gaggtctggc ggcagaacac
     3301 acacgcatat ccagttgaac ttatggtgat tataccatag attcgacctt tagggggaat
     3361 acgattaaat gcagacggcg ttaggcgatt cggcagtgag agtaattata ggacacgaac
     3421 tgtatgtcga tcgattgatt gaacaagcct atttctagat cagtgtatac ttacggcgaa
     3481 tcttattgag gagaaaaccc gatcaatgga cgaccaaacc aatttgcgaa cgaaggcgca
     3541 ggcgggcgcg caagattccg atcgctactt caagtacatg gccgagttcg taggtctctc
     3601 aaaaggcgac atcaatgcca ttcgacagac agcgccggtt atagaacatc acctgccgga
     3661 gatcgtcgcc aagttttact cgcacttgtt gcgttatccg cccacgcgca aattcttttt
     3721 aaagaatgac ggctcgatcg atcaagagta tgtcgagctg cgcatgcgcc atctgaccaa
     3781 cttctggctg cgtgccgcgc agggtaactt cgatgatgag ttcgcccgct acatcgatta
     3841 cgtcggccgc gcacacacct cgcacggcgc tgatccgaca atctatatcg ccgaacgtta
     3901 cgtcatcggt caagttggct tcatgcagca cgccatcgcc gaagccatca cgcgcgatct
     3961 gcggcacgtt aatgaagacc tggaaaccag cgccattgaa gcctgggatc gtttgatgat
     4021 ggtcattctg gaaatgttag cgcgggctta cggtaacgaa cgcgaagcgg aaactttcga
     4081 tgcgctggtc acgatcgatc agcaggcggt caatcggttg gcgcaagaag cgttcacatt
     4141 tgagcacgat aaagacaagc cggtggcaca caaggacata gtggttgggc gcgca
//
LOCUS       DASRTJ010000748         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_179998,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000748 DASRTJ010000000
VERSION     DASRTJ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179998"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..>4039)
                     /locus_tag="VFF70_15120"
     CDS             complement(301..>4039)
                     /locus_tag="VFF70_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZY46068.1"
                     /translation="LGTATQRQFVVTGATVNATSRAQSIASAGQIVVTPQLVDALMND
                     RTAFRFSPASDQHYTLESIVKPVESAARSDLVRSIDLFSADDIDSLRSISRLLDRLSP
                     YLPIGLLPRLISDPSHRQSSGEHRLVGVLFANFVGASEWIERSGPAQADQIAEQFNRY
                     YIKMQAAITKYGGIVNKIDLFDRGDELIALFGAPIAHEDDAERTVRSALEMQSTEKEA
                     GPMLIAQRIGVNTGIVFAGEVGSIDRREYTVMGDVVNLSARLMSAAADGELLLSNAMR
                     RKVSPFFEVADRGQVTVKGKAQPVSVYTIVGRRAQPEPVRGIRGLHSTLVGREKESKL
                     VHEAAVNLKRGKGSIISLIGEAGLGKSRLINEIRSEIAENVGAVQVPAQPQNEVSTTA
                     QLSPKQNNKVGAAVERLLDQNNFTWLEGHCLSYTQSVSYSAFNEVIRSALGIFETDTE
                     VEIWIKLRQRIDDLFSADQGADILPYLANFLSLPLSGSLAERVAYLKGETLQRQVIRA
                     VATLLEQMALRRPLILIFDDLHWADSASLTLLERCLTIPDRAPALIGLIYRPDRSHGC
                     WALGQLAARSYPHRYTEITLRPLDVTSGEDELLVSNLLSVPQLPIALAQLIARAEGNP
                     FYIEEIIRTLIDAGAIVRDPLENRWRIAGHITLESVPDSLQSIIMARIDRLAEEARRT
                     LQLASVVGRTFRYTVLHHLAAAVALADHLDTSLAALQRAELVREQTRIPELEYGFAQA
                     VFRDVAYESLLVRDRRVYHQLVGRQLEEINAEQKREEVYELLAYHYYLSDNQAKALTY
                     LIKAGDKTRLAYANNESINFYEQAEALVEELGTLEDKIAIAEGLGDVLYLVGEYESAL
                     KHYRQAVAYRTEPLPQAVLYCRIGAVYEKRGDYEGALNSTAKGIELLTPDRADTAEMA
                     RLLTLRCRIYHEQGQFDQAIESGQRALSIIEDTAYYQDIAQAHNELGNAYDVFSRPEE
                     ASIHLNHGLDILERISDGYGTAKVYNNLAVIYSQTDLARAEEYFKRGLETMQRFGDVW
                     NEAVSYLNLGGISFLRGDYPQAVGYYQRSLEMKERLGDNMGIADCHINLGEVYRTQGD
                     LPQAIDHLEQALFIARQIGASQSEAECQRQLGECYVENSEPEKALSILREALTRAQTI
                     SDLKEAGIIYRVMGNAYLRQRDLEAAVLHLGQSVKVLVELNREFDVASARYDFALALI
                     EANQTDRAQTELNAAVILFEKLELPQEKAKAQAAIDRLGSI"
BASE COUNT          857 a         1072 c         1127 g          983 t
ORIGIN      
        1 atgatgtact gtgagacttt tagtcagttg acagcaggct ggctgggcgc cgggttacag
       61 attgggggac tgctgaccat tttaattttc acagtattga atcgtcggat gaatggtatt
      121 tgacagatgt cattcctatc ctgtgtacca ccgctcatcc ctaggtggtc agcataggcg
      181 acattctggt tgggttaggg ctgttctgtg tcattgtttg gggagtgcac acgttaccac
      241 cacttctagc gtgaggtgat tgtaaaatga ttaatctttt gaccagccta gagcattcga
      301 ttaaattgac cccaatcgat cgatcgccgc ttgcgctttg gctttttcct gaggcagttc
      361 caatttctcg aaaaggatca ccgctgcatt gagttctgtc tgcgcccgat cggtctggtt
      421 tgcctcgatg agcgccagag caaaatcata acgcgccgaa gcgacatcga actcgcgatt
      481 caactccact aacactttga cgctttgtcc cagatgcagc acggccgctt cgagatcgcg
      541 ctggcgcaga taggcattcc ccatcacgcg atagatgatg cctgcttctt tgagatcgct
      601 gatcgtttgg gcgcgtgtca aagcctcacg caagattgat agagctttct ccggctcgct
      661 gttttccaca tagcattcac ctaattggcg ttgacattcg gcttcgctct ggctcgcgcc
      721 gatctgccgc gcgatgaaca aggcctgctc caaatgatcg atcgcctggg gcaggtcgcc
      781 ctgtgtgcga tagacctcgc ccagattgat atggcaatcg gcaatgccca tattatcgcc
      841 gagccgttct ttcatctcga gactgcgctg ataatacccg accgcctgcg gataatcacc
      901 ccgtaggaaa ctgatgccac ctaagttgag ataacttacg gcttcattcc acacatcgcc
      961 gaaccgttgc atggtttcaa gtccgcgctt gaaatactct tctgcgcggg cgagatcggt
     1021 ctgtgaataa atgacggcca aattgttgta gactttcgcg gtgccgtatc cgtcgctgat
     1081 acgctccaag atgtccaagc cgtgattcaa gtgaatgctg gcttcctctg ggcggctgaa
     1141 gacatcgtag gcgttgccca gttcattgtg ggcttgtgcg atgtcttgat aataagccgt
     1201 gtcttcaatg atcgacaacg cccgttggcc ggattcaatt gcttgatcga attgaccttg
     1261 ctcatgatag atgcggcaac gaagagtcag cagccgggcc atttcggcag tatccgctcg
     1321 atcgggtgtg agcagttcaa tgcccttcgc cgtgctgttg agcgcgcctt cgtaatcgcc
     1381 gcgcttttca tatactgcgc cgatgcgaca atagagaacg gcttgaggga gcggttcagt
     1441 gcgataggcc acggcctgcc ggtagtgctt gagcgctgac tcgtattcgc cgacgagata
     1501 caacacgtcg cccagccctt ccgcaatcgc gatcttgtcc tcaagtgtgc cgagttcttc
     1561 gactaaggct tcggcctgtt cgtagaagtt gatcgattca ttattggcgt aagctaatcg
     1621 ggttttgtca cccgctttaa tcagataggt tagcgctttg gcttgattgt ccgataagta
     1681 gtagtgatag gccaataact cataaacttc ctcgcgtttt tgttctgcgt tgatttcttc
     1741 caactggcga ccgactaatt ggtggtacac gcgtcgatcg cgcacgagca gactttcata
     1801 ggccacatcg cggaacacgg cttgcgcgaa gccgtattcc aattcaggga tgcgtgtttg
     1861 ttcgcgcaca agttcggcgc gttgcagtgc agcaaggctg gtatctaagt gatcagccag
     1921 cgccaccgca gctgccagat ggtgcaagac tgtgtatcgg aatgttcgac cgacgactga
     1981 ggcaagttgc aaggtccgcc gcgcttcttc agccaggcga tcgatgcgag ccattatgat
     2041 cgactgcagc gagtccggca ccgattccag cgtgatgtgg cccgcgatgc gccagcgatt
     2101 ctcgagcggg tcacgcacga tcgcgcccgc atcgattagg gtgcggatga tctcttcaat
     2161 ataaaacgga ttgccttcgg cgcgggcgat cagctgcgct aaagcgatcg ggagttgcgg
     2221 cacgctcaat aaattgctga cgagcagttc gtcttcgccg gaagtaacat cgagcgggcg
     2281 cagcgtgatt tcggtatagc gatgcggata agatcgcgcg gcgagttgac ccaacgccca
     2341 gcagccatgc gatcgatcgg ggcgatagat caatccgatc agcgccggag cccgatcggg
     2401 aatggtgagg cagcgttcca gcaatgtgag tgacgcacta tcggcccaat gcagatcgtc
     2461 gaagatcaaa attaacggac ggcgcagcgc catttgttca agcaaggtcg caaccgcgcg
     2521 aatgacttgc cgctgcaagg tttcgccttt caggtacgcg acgcgttcgg cgagcgatcc
     2581 gctgagcggc aaacttaaaa agttggcgag atacggcaga atgtctgcgc cttgatcggc
     2641 tgaaaacaga tcgtcgatgc gctgacgcag tttgatccag atttccactt cggtgtcggt
     2701 ttcaaaaatg ccgagcgcgg atcgaatgac ttcgttgaag gcggaataac tgacgctctg
     2761 cgtgtaagat aaacagtggc cttccagcca cgtgaaatta ttttgatcga gcagccgttc
     2821 aactgctgcc ccaactttgt tgttttgttt gggtgacagt tgcgctgtcg tgctaacttc
     2881 attttgtggt tgggctggca cttgcacggc tccaacattt tctgcgatct cagatcggat
     2941 ttcgttgatt aagcggcttt tgccgaggcc tgcttcgccg atcaaagaaa tgatggagcc
     3001 tttgccgcgt ttcaaattca cggcggcttc atgcacgagt ttggattctt tttcgcgccc
     3061 gaccagcgtt gaatgcaacc cacgaatgcc gcgcaccggt tccggctgcg cgcgccgtcc
     3121 gacgatggta tacactgaaa cgggctgggc cttgcctttg acggtaactt gtccgcgatc
     3181 ggccacttca aaaaacggcg agactttgcg gcgcatggcg ttcgacaaca agagttcgcc
     3241 gtcggcggcg gcgctcatca accgcgccga taaattcacg acgtcgccca tcacggtgta
     3301 ctcgcgtcga tcgatcgagc cgacttcgcc tgcgaaaacg atgcccgtat tcacgccaat
     3361 gcgctgcgcg atcaacatcg gcccggcttc tttctccgtc gattgcatct ccagcgctga
     3421 tcgaaccgtc cgctccgcat cgtcttcatg tgcgattggc gcgccgaata aggcgatcaa
     3481 ttcgtcgccg cgatcgaata gatcgatctt gttgacgatg ccgccgtatt ttgtaatcgc
     3541 agcttgcatc ttgatatagt agcgattgaa ttgttctgca atctgatcgg cttgagctgg
     3601 acccgatcgt tcgatccatt cgctggctcc gacgaagttg gcaaacagca cgccgaccaa
     3661 gcgatgctcg ccgctggatt gtcggtggga tggatcggag atcaagcgcg gcagcagacc
     3721 gatcggaaga taagggctca ggcgatcgag caagcggctg atcgatcgca gtgaatcaat
     3781 gtcgtccgct gagaagagat caatcgatcg aactagatct gatcgcgctg ctgattcgac
     3841 cggtttcacg atcgattcaa gcgtgtaatg ctgatcggag gcgggcgaaa atcgaaatgc
     3901 ggttcgatca ttcatcaaag catcaaccag ttgaggcgtg acgacaatct ggccggcgga
     3961 tgcgatcgat tgcgcgcgcg aagtggcatt gacggtggcg ccggtcacga cgaactggcg
     4021 ttgcgttgca gtgccgagc
//
LOCUS       DASRTJ010000749         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_221168,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000749 DASRTJ010000000
VERSION     DASRTJ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221168"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFF70_15125"
     CDS             <1..684
                     /locus_tag="VFF70_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZY46069.1"
                     /translation="VLFTVAAWLATPLLPILQWIAQLIFKVLMAGLTLLNNLGLSIDR
                     LKASNDFNDFLNSPTFVTISRSAILMAILIVIALVAIWSLRRSGLLGKKQPYEIRESI
                     ASRQLLWSQLKNLLARLWSRSSSVTHSMYLTLDGSADDPRLIIRRAYRSMLDWARLNG
                     RARFPYQTPLKYADALGADLPHAKEAIASLTQSYVLARYSIDPLPPDEARRAQAALDR
                     LLVTPPPVN"
     gene            798..1319
                     /locus_tag="VFF70_15130"
     CDS             798..1319
                     /locus_tag="VFF70_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZY46070.1"
                     /translation="MALYEFEGKRPQIGKTSFVPETADVIGDVVIGEGCFIGVGARIR
                     GDYGSIRIGDRTSVQENVVIHAREGDQTIVGSNVQLGHGCILHNCTVKDNAVIGVGAI
                     LTDFSIVGDWAIVGEGAVVRGEVPDGSIAVGVPAKVIGHVTDKHKEEWQYYKDKYAVL
                     ASVRYPKGLKRID"
BASE COUNT          305 a          409 c          412 g          384 t
ORIGIN      
        1 gtgttgttta cagttgcagc ttggctggcc acgccgctgc tgcccatctt gcagtggatc
       61 gcacaattga tctttaaggt tctcatggca ggtctgacgc tgttgaacaa tctgggcctc
      121 tccatcgatc gtctcaaagc atccaatgac ttcaatgatt ttctgaactc gcccaccttt
      181 gtcaccatca gccgcagcgc gattctcatg gcgatcctga tcgtgatcgc gctggtggcc
      241 atttggagct tgcgtcgatc gggtttgctt ggcaagaaac aaccctatga aatccgcgag
      301 agcattgcat cgcgccagtt attgtggtcg cagctcaaga atttgctggc gcgcttgtgg
      361 tcgcgatcct cttcagtcac gcactccatg taccttaccc tcgacggctc cgccgacgat
      421 ccgcgtttga tcatccgccg cgcctatcga tcgatgttgg attgggcgcg cttgaatgga
      481 cgtgcgcgct tcccatatca gacacccttg aagtatgccg atgcgttggg cgcggacctg
      541 cctcacgcca aagaagcgat tgcctcgctc acgcaatctt acgtcctcgc ccgttactcg
      601 atcgatccgc tgccgcccga cgaagcccgt cgtgcgcaag ccgcgctcga tcgattgttg
      661 gtgactccgc cgcctgtaaa ctgatcttcg atccaatttg ttataattgc ctcagtttga
      721 tattgtgtac ggcgtatttc gtgtcacgca gcatgcaata tgccttactc gttactcatt
      781 acttgtcagg agtttccatg gccctctacg aattcgaagg caagcgaccg caaattggca
      841 aaacttcatt cgtccctgaa acagcagacg tgatcggtga cgtggttatt ggtgaaggct
      901 gctttatcgg cgtgggtgcg cgcattcgcg gcgactacgg atcgatccgc atcggcgatc
      961 gaacgtcggt gcaggagaac gtcgtcattc acgcgcgcga gggcgatcaa acgatcgtcg
     1021 gatcgaacgt gcagttgggg catggctgta tcttacacaa ctgcacggtg aaggacaatg
     1081 ccgtgatcgg cgtcggcgcc atcttgaccg acttctcgat cgtcggcgat tgggcgatcg
     1141 tcggtgaggg ggctgttgtg cgcggtgaag tgccggatgg atcgatcgcc gttggggtgc
     1201 cagcgaaagt aattgggcat gttaccgata agcacaaaga agaatggcag tattacaaag
     1261 acaaatacgc tgtcctggcc tcggtgcgtt atccgaaagg cttgaagcgg atcgattgag
     1321 tcacccctgt cattccgagc gcagcgagga atctctatca cgattggctc atactgctga
     1381 tagtgctaga gattcctcgt cgctacgctc ctcggaatga catgcagtca ctatcctttc
     1441 gactttcgat tcgaattgcg gtatactccc cgcattgaga ttagattcat agtttgggtt
     1501 gatcctggga
//
LOCUS       DASRTJ010000750         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_180360,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000750 DASRTJ010000000
VERSION     DASRTJ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180360"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1531
                     /locus_tag="VFF70_15135"
     CDS             <1..>1531
                     /locus_tag="VFF70_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46071.1"
                     /translation="PFIGAFIEQTGVTSQINGANWPARSLGLPGAAHILQIDGTAVQT
                     AQDIRAIIESKLIGDHVSIDYALPDGSRNSLSNVPLESFPLQDLLSNFWLPYLLGVGY
                     FALGWWTYRARGETRAARTFAALCCGVALTLGLFFDMNTTQWFVRVWTAAIPLTGAAS
                     VSLALLFPEQIPLVDRHPRLRFIPYGPAVLIALFAEIVLFDQNDPFAYFVGWRWCYLA
                     IATGILTMFITAIYRQRRSRTMLARQQARIVVWGSVVAFGPIALWSLQAALTLSLPFS
                     AGLFFTPLIAFPLAIAYAIVRYQLLDVDRIVSQGLGYTALTAIVVGGYLLIVNVLSQV
                     LRLQLGAADPLPLAIFVFLLVIGLNPLRGRIQRTIDRIFFRDRVDYRQALELFSHELT
                     GTLDLRSILLKVRSNLQATLHPDRMLVHLYDEESRSYFEVSDDEFEAATLGIESPLAQ
                     RLARENHVLYLMPNAPLPIDLQPDRARVTSLNTPVFVPLRSRDQLSGWLSIGQKLSGN
                     V"
BASE COUNT          274 a          446 c          422 g          389 t
ORIGIN      
        1 gacctttcat cggcgcgttc attgaacaga ccggcgtaac cagtcaaatc aatggcgcaa
       61 actggccggc tcgatcgctg ggcttgcccg gcgcggcgca cattcttcag atcgatggca
      121 ctgccgtgca gactgcgcag gatattcgcg cgatcatcga atcaaaatta atcggcgatc
      181 acgtctcgat cgattatgcg ctgcccgatg gatcgcgcaa cagcctctcg aatgttccac
      241 tggaatcatt tccactgcaa gatttgctgt ccaatttttg gctgccgtat ttattgggcg
      301 tgggctattt cgcgttaggc tggtggacct atcgggcacg cggcgaaaca cgcgcggctc
      361 gcaccttcgc cgcattatgc tgcggcgtcg cgctcacact cggcctgttc ttcgatatga
      421 atacgacgca gtggttcgtg cgcgtctgga cggcggccat acccttaacg ggcgcggcgt
      481 cggtctctct ggcgctcttg ttccccgaac aaattccgtt agttgatcgg cacccgcgtt
      541 tgcgcttcat accttacggg ccagcggtct taatcgcgtt gttcgccgaa attgtcttat
      601 tcgatcaaaa tgatccgttt gcttacttcg tcggttggcg ctggtgctat ctcgccattg
      661 ctaccggtat tctcacgatg ttcattaccg ccatctatcg ccaacgccgc agccgcacca
      721 tgctcgcacg gcagcaagcg cgcattgtcg tgtggggcag cgtggtggcg tttgggccga
      781 tcgcgctctg gtcattgcag gcggcactca cgttaagcct gcctttttcc gccggactgt
      841 tcttcacgcc gttgatcgca tttccgttgg cgattgcgta tgcgattgtg cgttatcaat
      901 tgctggatgt cgatcgcatt gtcagtcaag gtctcggcta caccgcttta accgcgatcg
      961 tcgtgggcgg ctatttgctg atcgtcaacg tcctgagtca ggtgctgcgc ctgcaacttg
     1021 gtgcggccga tccactgccg ctggcgattt tcgtttttct gctggttatc ggtctcaatc
     1081 cgctgcgggg ccgaattcaa cgcacgatcg atcggatctt cttccgcgat cgcgttgatt
     1141 atcgccaggc actggaatta ttttcgcacg aactcaccgg cacgctcgat cttagatcga
     1201 tcctgctgaa agtccgatcg aatctgcagg ccacgctgca tcccgatcgg atgctggtgc
     1261 atctatacga cgaagaaagt cgatcgtatt tcgaggtcag cgatgatgaa ttcgaagcgg
     1321 caacgctcgg catcgaatcg ccgttggcgc agcggctggc gcgcgagaat cacgtgctgt
     1381 acttgatgcc aaacgcgcca ttgccgatcg acctgcagcc cgatcgcgcg cgcgtcactt
     1441 cattgaacac gccggtgttt gtgccgctcc gcagccgcga tcaactcagc ggctggttgt
     1501 cgatcggtca aaaactttcg ggaaatgtat a
//
LOCUS       DASRTJ010000751         7687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_180429,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000751 DASRTJ010000000
VERSION     DASRTJ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180429"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFF70_15140"
     CDS             complement(<1..569)
                     /locus_tag="VFF70_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46072.1"
                     /translation="MGQQAKMSQQAKAMLAGIRGLLAGYPFITLAAGLVVGLLIGLVV
                     LGWWLWPVRWTDARPSDLSSKAEIQYQQDFFRLAADAYQYGRPIQDIAKWFGDGWTRA
                     QILTAIDNVVVSSEEKSSIQALRSALSDPNVPVGGDQLSKSAGTGGTSPLLALVGLAV
                     IAIIAIALLIIILQRLRSRQMLPPEEPITL"
     gene            697..2274
                     /locus_tag="VFF70_15145"
     CDS             697..2274
                     /locus_tag="VFF70_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY46073.1"
                     /translation="MIIKLIASLVVFILASNTDSFHNVIIIGGGLAGLSAALHLAERG
                     LQPIVLEADPDRAGGRVSGGQAVELTQADQLWCFPAEHGIHGFWSQYHNLRGMLNRHH
                     ILPQFVAAKQEEWIIAHGSRIKRAEAGSHIRGSWVPAPFHYLNLLIRPSFLRILDIFD
                     WFALPFVLAKLLVALSIDPLREGKSLQGMSLAQFTWGWTPNLRAFFAALARSGLSAHP
                     EQVPLDGFIAFLRFYTLTRRDAWAFEYFVADSGSALIDPIVHKVIDLGGQIEFDQAVQ
                     QIDREADTWIIRTNRSSFRARSIILAVDAPNAKQILCQSPITRVAATRFEFPIGIATA
                     ILRVWFDRSPRNGPEAGIFSGDFTLDNFFWLDRFQTKFMNWRAATGGSAIESHVYGPP
                     EVLKQPDSNLISTLLNDIHRAWPELNGHSIHYTLQRNKPAHTLFKIGTTESRLGTETL
                     WPDLFVCGDWIRHPTPALFLERACITGIDAANAVLESNQIDRFLLIPHTPPEPLARGI
                     EEIMHGLRRGLHRLRRN"
     gene            2420..3343
                     /locus_tag="VFF70_15150"
     CDS             2420..3343
                     /locus_tag="VFF70_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZY46074.1"
                     /translation="MRTIFLWLSHRKFFRTLAAKLGFVRRAVMRFVAGETIDAAIRAI
                     KELNAIGITTTLDHLGENVETEADATRAADDYIDVLEAISKNDVKSHVSIKLTALGLD
                     LGDDFCCANVARVIAKAKSIGSFVRIDMEGTPYTERTLAIYRSLRKEFDNLGIVIQAY
                     LYRTEKDIDSLCAEGAKVRLCKGAYKEPADKAFPKKADVDANYIRLTKKLLSAKARAK
                     GTRGAIATHDPKMIEATRQYAAEANVPHDEFEFQMLFGIRRELQRQLASEGYAMRVYV
                     PYGTEWYPYFMRRLAERPANVWFVLSNMFKK"
     gene            3485..4219
                     /locus_tag="VFF70_15155"
     CDS             3485..4219
                     /locus_tag="VFF70_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZY46075.1"
                     /translation="MDIHNKFALVTGGAHRVGQAIALALARSGVHVAITYRLSVEAAH
                     ATVDQIKAMGVRSIALHCDQRNFTEIQSLFVSLQREFDHLDILINSAALMERKTVLDV
                     TPDDFDRTIETNLRGPFFIAQAAAKWMKQSGVAGSIVNIADLSAIHPWPSYIAHTISK
                     SGIVAMTHTLALALAPLIRVNAVAPGAIMKPVDWDDARWSKLAGTLPLQRTGSAEDAA
                     NAVLFCVQSEFMTGETIVIDGGRSLK"
     gene            4216..4605
                     /locus_tag="VFF70_15160"
     CDS             4216..4605
                     /locus_tag="VFF70_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46076.1"
                     /translation="MKKVARPKPVPSKFQPIFEQLKKILRRYERRLTVTAYSSTGYSL
                     NAAFSPKYKKEIFFGALQINKNDVSYHLMPVYVFPALLKGISPVLTKRMHGKSCFNFT
                     SIDDRLLAELSTLTEKCFKKFEDGKMI"
     gene            4691..5185
                     /locus_tag="VFF70_15165"
     CDS             4691..5185
                     /locus_tag="VFF70_15165"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY46077.1"
                     /translation="MSAKHDLIRKRLRDEGDKLVALFDALTSVQWQQTLYTDGMTWSI
                     KDLLAHQISAERKFIFYGHDILNGGEGAPEGFQINAFNNAEVASMIDRTPDQLLTDMR
                     AVRRATIDFVDNVQDSDFDRRGRHPFFGMMSIEEMFKLIYRHNMLHARDVRRAIGGHD
                     HDGS"
     gene            5172..5648
                     /locus_tag="VFF70_15170"
     CDS             5172..5648
                     /locus_tag="VFF70_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZY46078.1"
                     /translation="MTDRKHQLHDHLNATREALLNIVGDLQPDDWNKQVQGDDGHWTV
                     RQMMLHLATSESGQIGTGKSIVAGQPTVPDDFDLNRYNNRQVEKNAAKMPPEILFGMA
                     ESRQKLLAFLDEVSEADLDKRGKHARGDVISLEQLFYRIGEHEAEHAELIRQAVRK"
     gene            5655..6140
                     /locus_tag="VFF70_15175"
     CDS             5655..6140
                     /locus_tag="VFF70_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY46079.1"
                     /translation="MIQLIPMTQVEFDKFLSVEIEEYAQEHVKAGNWHPDEAIQLSKA
                     EHAKLLPNGLASPNQYLYSIQATDSGSNIGMIWFAEQQQGPNHYAFIYDFRIDEAQRG
                     KGYGQDALSAIEEKVKALGLDTISLHVFGHNQMARSLYEKVGYEITNVNMSKKLTHDI
                     A"
     gene            6127..7017
                     /locus_tag="VFF70_15180"
     CDS             6127..7017
                     /locus_tag="VFF70_15180"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZY46080.1"
                     /translation="MTLRKLIAFIQLTRPLFLVGGVVMYSLGVFMARSQGTPIDLPRA
                     LLGQAIITSIQLMTHYSNEYYDLETDRLIGADRTFFSGGSGVLPAGLLSPLVAQQAMI
                     IFAAIGFIAIIVGTILSPLVGLIGILGLLGSYFYSAPPLRLSGSGWGELTASIIVAVL
                     TPLTGYVLQTNRIDPIVFQIAAPLVLLHLAMVLTFELPDYAADLATHKQTLAVRLNRN
                     TIGWLYTVLIIAAFALIAITGTSFNRFVWISLPLAAWQITGGVWRIKRGWTHLGWLTF
                     GGLALFALSASLWLIGFILT"
     gene            7166..7405
                     /locus_tag="VFF70_15185"
     CDS             7166..7405
                     /locus_tag="VFF70_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46081.1"
                     /translation="MCSAPLLQARLIDQLPNGMRRGFMGLSTDAQRCLQFVRSTPGMA
                     VALAGMSQVEHVEENMTTAQVAPLTLEQIQALLIR"
     gene            7407..>7687
                     /locus_tag="VFF70_15190"
     CDS             7407..>7687
                     /locus_tag="VFF70_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HZY46082.1"
                     /translation="MPELSTVLSPNIALKKLFDHLPRSSAIEAIETANGLQRVLAESP
                     IATMPLPSFPRSTMDGYAVRAADTFGASEALPAYLKVIGESPMGRAPSI"
BASE COUNT         1813 a         2060 c         2000 g         1814 t
ORIGIN      
        1 agcgtaatcg gctcttccgg cggcagcatt tggcgactgc gcaagcgctg taaaatgata
       61 atcaacagcg caatcgcgat gatggcaatc accgctagtc caaccaaagc cagcagagga
      121 gaagtgccgc ccgtgcctgc acttttgctc agctggtcgc caccaaccgg aacattcgga
      181 tcggataagg cactgcgcag cgcttgaatg ctggactttt cttctgatga aacaacaacg
      241 ttatcaatcg cggttaagat ctgcgctcgc gtccaaccat cgccgaacca cttcgcaata
      301 tcttgaatcg gtcggccata ttggtatgcg tcagcggcca aacggaagaa gtcctgttgg
      361 tattgtatct cggctttgga actcaaatcc gatggtcgcg catccgtcca acgcaccggc
      421 cacagccacc aacccaacac gactaagccg atcaagagac cgacgactaa gcccgcagcc
      481 aacgtgataa acggataccc ggcgagtaag ccacgtatgc cagctaacat tgctttggcc
      541 tgttgactca tttttgcttg ttgacccata gcctgactcc ttagtgcaga tcgaatcccc
      601 taaagtatag attggacttc actctaagtc aaatcgcgcc tcgactgaaa tgtttgtttt
      661 ctgtttgacg ttcttgcaga tcgatcagcc tatgatatga ttataaaact cattgcatcg
      721 ttggtcgtct tcattttggc aagcaacact gattcctttc acaacgtcat catcatcggc
      781 ggcggcttgg ccggactctc cgccgcgtta cacctggccg agcgtggact gcagccgatc
      841 gtcctcgaag ccgatccgga tcgcgcgggc ggccgagtat ctggcggcca ggctgttgag
      901 ctcacgcaag cggatcagtt gtggtgcttt cccgccgagc atggcattca cggcttttgg
      961 tcgcagtatc ataatttgcg cggaatgctc aatcgacatc acatcctccc gcaattcgtc
     1021 gcggccaaac aagaagaatg gatcatcgcg catggctcgc gcatcaaacg cgcggaagcc
     1081 ggcagccaca ttcgcggcag ttgggtgccc gcgccgtttc attatctcaa cttgttgatt
     1141 cgacctagtt tcttgcgcat cctcgacatc tttgattggt tcgcgctgcc ctttgtctta
     1201 gcgaaactgc tcgtcgccct atcgatcgat ccactgcgcg aaggaaaatc tttgcagggt
     1261 atgtcgctcg cgcaatttac ctggggctgg acgccgaacc tgcgtgcctt cttcgccgcg
     1321 ctcgctcgca gtggattgtc cgcgcatccc gagcaagtgc cgctcgacgg cttcatcgcg
     1381 tttctgcgtt tttacacgtt gacccgccgc gatgcgtggg cttttgaata tttcgtcgca
     1441 gattccggca gcgccttgat cgatccaatt gtacacaaag tgatcgatct cggcgggcag
     1501 atcgaattcg atcaagcggt ccaacagatt gatcgagagg cggatacctg gatcatccgt
     1561 accaaccgat cgagcttccg cgcccgatcg atcattctcg ccgttgatgc gccgaacgcg
     1621 aaacaaattc tatgccagag tcccatcacc cgagtagccg cgactcgatt tgaatttccg
     1681 atcgggattg cgactgcgat cctgcgcgtg tggttcgatc gatcgccgcg taacgggcca
     1741 gaggcgggta tcttcagcgg cgatttcacg ctcgataatt tcttctggct cgatcggttt
     1801 caaacgaagt ttatgaactg gcgcgccgcc accggtggca gcgcgatcga atcacatgtc
     1861 tatggaccgc ccgaagtgct gaaacaaccc gattcaaact tgatcagcac attgttgaat
     1921 gatattcatc gcgcatggcc ggaactcaac ggacattcga tccactacac actgcagcgc
     1981 aataaaccag cccatacttt attcaagatc ggtacgaccg aatcgaggtt aggcactgaa
     2041 acactgtggc ctgacctctt tgtgtgtggc gactggattc ggcatccaac ccctgccttg
     2101 tttttggagc gtgcctgtat caccggtatt gacgccgcca acgctgtttt ggaaagcaac
     2161 caaatcgatc gcttcttatt gatcccgcac accccgccgg agccactcgc ccgcgggatc
     2221 gaagaaatca tgcacggctt gcgtcgagga ttgcatcgac ttcgccgcaa ttagattagt
     2281 catttttcat tagatcattt atcattgttc acaaagtccc caaagcgaaa gtggagaggg
     2341 attggtcata cgccatttgt cattgtaaaa tatcactgcg ccacctgttc agcacaaccc
     2401 ctcatttgga gcctaacgca tgcgaaccat cttcctctgg ctgtcccatc gaaaattctt
     2461 tcgcactctt gccgcaaaat tgggctttgt ccgtcgcgcc gtgatgcgct tcgtcgccgg
     2521 tgagacgatc gacgctgcca ttcgcgccat caaagaactc aacgcgattg gcatcaccac
     2581 cacactcgat catctcggcg aaaacgttga aactgaagcc gacgctacac gcgctgccga
     2641 cgattacatt gatgtcctcg aggccattag caaaaacgac gtgaagtcgc acgtctcgat
     2701 caagttgacc gcgctcggcc ttgatctggg cgacgatttc tgctgcgcga acgtcgcgcg
     2761 cgtgatcgcc aaagccaaat cgatcggctc attcgtgcgg atcgacatgg aaggcacgcc
     2821 ctataccgaa cggacgctgg cgatctatcg gagtttgagg aaagaatttg ataacctcgg
     2881 catcgtcatt caagcctatt tgtatcgcac tgaaaaggat atcgactcgt tgtgcgctga
     2941 gggcgcaaag gttcgattgt gcaaaggcgc gtataaagaa cctgcggaca aagctttccc
     3001 caagaaagca gatgtcgatg cgaactatat ccgtctgaca aaaaagctgc taagcgctaa
     3061 agcccgcgcc aaaggcacac gcggtgccat cgcgacgcac gatcccaaga tgatcgaggc
     3121 gaccaggcaa tacgccgctg aagctaatgt accgcacgac gaattcgaat tccaaatgct
     3181 attcggcatt cgacgcgagt tgcagcggca gttggcgagc gaaggttatg ccatgcgagt
     3241 ctatgtgccg tacgggacgg aatggtatcc ttatttcatg cgccggctgg ccgagcgtcc
     3301 agcgaacgtg tggtttgtgt tgagcaatat gtttaagaaa taagactata gcattctgtg
     3361 cggagttgcg gactcaaaga gatcgcaacc cagtcgaaga agcgaacgag atcaacgcgc
     3421 ccttcgactg cggagtgagg tctgcgaccc cagtactccg ctcaggctgc ttctacattt
     3481 tcatatggac attcacaaca aattcgcgct cgtcacgggt ggcgcacatc gagtaggtca
     3541 agcgattgcc ttggcattgg cccgatcagg cgtgcacgtg gcgataacct atcgcttgtc
     3601 cgtggaagca gctcatgcca cggttgatca gatcaaagcc atgggcgtgc gatcgatcgc
     3661 gctgcactgc gatcaaagaa actttactga gattcagtcg ctctttgtat ctctccagcg
     3721 cgagttcgat catctcgata ttctgatcaa cagcgcagca ctaatggaac gtaagactgt
     3781 gttggatgta acacctgatg atttcgatcg gacgatcgaa acgaatttgc gcgggccgtt
     3841 cttcatcgcg caggcggccg caaagtggat gaaacaatcg ggcgtggcag gatcaatcgt
     3901 gaacatcgcg gatctatcgg ctattcatcc gtggccgagt tacattgccc ataccatcag
     3961 caaaagcggc atcgttgcca tgacacacac gctggctctc gcgctggctc cattgatccg
     4021 ggtgaatgca gttgcgccgg gggcaatcat gaagcccgtt gattgggacg acgctcgctg
     4081 gagtaagttg gctgggacat tgccgctgca gcgcaccgga tcagcagaag acgccgcgaa
     4141 cgccgttttg ttttgtgtgc aaagcgaatt catgacgggc gagacgatcg tgatcgatgg
     4201 cgggcggagt ttgaaatgaa gaaagttgcc aggccaaaac cggtaccgag caaatttcaa
     4261 ccgatcttcg agcaattgaa aaagattttg cgccgatacg aacgtcgctt gacggtaacg
     4321 gcgtactctt caactggcta ttcactcaac gccgcattct cgccgaagta taagaaggaa
     4381 atcttttttg gcgcccttca gattaacaaa aacgatgtaa gttatcactt gatgccggtc
     4441 tatgtttttc cggcgttgct gaaaggcatc tcgccggtat tgacaaaacg gatgcatggt
     4501 aaatcctgct tcaacttcac ctcgatcgat gaccgactac tagctgaact ttcaacactg
     4561 actgaaaagt gttttaagaa gttcgaagac gggaaaatga tttaagtttc agggtgcgaa
     4621 ccctaataga gtacaatgat gccggggcaa gtttcggcat cattttttat cttcccacag
     4681 gcggccgagg ttgagcgcaa aacacgattt gatccgcaaa cggttacgtg atgaaggcga
     4741 taagttagtc gcgctgttcg acgcgctaac gtccgttcag tggcagcaga ccctatatac
     4801 cgatggaatg acctggtcga tcaaagactt gctggcgcat caaatttccg ctgaacgcaa
     4861 attcattttc tatggacacg atattctcaa cggcggcgaa ggcgcacccg aaggttttca
     4921 gatcaatgcg ttcaacaatg ccgaagttgc ttcgatgatc gatcggacgc cggatcaact
     4981 attgaccgac atgcgagcgg tgcggcgggc gacgatcgat tttgtggaca acgttcagga
     5041 ttcggacttc gatcggcgtg gacggcatcc attctttggt atgatgtcga tcgaagaaat
     5101 gttcaagttg atttatcggc acaatatgct gcacgcccgc gatgtgcggc gggcaattgg
     5161 aggccatgac catgacggat cgtaaacatc aactgcacga tcatttgaat gcgacgcgcg
     5221 aagcgctgct gaatatcgtc ggtgacttgc agccggacga ttggaacaag caagtgcaag
     5281 gcgatgacgg ccattggacg gtaaggcaga tgatgctgca cttggccaca tccgaatcgg
     5341 gacaaatcgg tacgggcaaa tcgatcgtcg ccggtcagcc gaccgtgccg gacgacttcg
     5401 atctcaatcg ctacaacaat cgacaggtgg agaaaaatgc ggctaagatg cccccagaaa
     5461 ttttattcgg catggcagag tcgcgtcaaa agttattagc gttcttagac gaagtcagcg
     5521 aagccgatct cgataagcgt ggtaagcacg cgcgcggtga tgtgatttcg ctggagcaac
     5581 tcttttatcg aattggcgag catgaggcgg agcatgcgga gctgatcaga caagccgtga
     5641 ggaaatgatt cgttatgatc caactaatac cgatgaccca agtcgagttt gataaatttc
     5701 tctccgtcga gatcgaagag tacgcgcagg aacatgttaa agcgggtaac tggcatcccg
     5761 atgaagccat ccaattgtcg aaggccgagc atgctaagtt gctgcctaac ggcttggcgt
     5821 cgcccaatca gtatctgtat tcgatccaag cgacagactc gggatcgaac attggcatga
     5881 tttggtttgc cgagcagcag caaggtccaa atcattatgc cttcatctat gatttcagaa
     5941 tcgatgaggc acagcgcggc aaagggtatg gccaggacgc cctgtcagcc attgaagaaa
     6001 aagtgaaagc tctagggctc gatacaatct cgctgcacgt ttttggccac aatcaaatgg
     6061 ctcgatcctt gtacgaaaaa gtcggctatg aaatcaccaa tgtgaatatg tcaaagaaat
     6121 tgacgcatga cattgcgtaa actgatcgcc ttcatccaac tgacccgccc attgtttctg
     6181 gtgggcggcg tcgtgatgta ttctctcggc gtattcatgg cgcgatccca aggcacccca
     6241 atcgatctgc cacgcgcgct gctcggtcaa gccatcatta cttcgatcca attgatgacc
     6301 cactactcga acgaatacta cgatcttgaa accgatcggt tgatcggcgc tgatcggact
     6361 ttcttttcag gtggcagcgg cgtcttacct gcgggattgc tctcaccatt agttgctcaa
     6421 caggcgatga tcatctttgc agccatcgga ttcatcgcga tcattgtcgg aaccattcta
     6481 tccccgctcg tcggattgat cggcatcctc ggtctgctgg gatcatattt ttattctgcg
     6541 ccgccgcttc gactttcggg cagtggctgg ggcgaactca ccgcctcgat catcgtcgct
     6601 gtgctcacac ccttgaccgg gtatgtgctg caaaccaatc ggatcgatcc gatcgtattt
     6661 caaattgccg cgccgttagt cttgctccat ctggcgatgg tgctcacttt cgaattgcct
     6721 gactatgcag ccgatttagc cacgcataaa cagacactcg ccgtccgctt aaaccgcaat
     6781 acgatcggct ggctgtacac cgttttgatc atcgccgcat ttgcgttaat cgcaatcaca
     6841 ggcacatcct tcaatcgttt cgtgtggatc agcctgccac tggccgcgtg gcagatcact
     6901 ggcggagtgt ggcggatcaa acgaggttgg actcatttgg gatggctcac ttttgggggt
     6961 ctcgcactct ttgcactctc agccagtttg tggctaatcg gctttatact gacctgaacc
     7021 ttgcgaaggt agttttgatc ggcaacccca cccgcgttga gttttgatcc ccatttacat
     7081 ccgacgcagg tcgcactata atcaaatcat caaaagcgag atgatgtcga tctttgaagc
     7141 gactgagatg tcaggggtga cggtgatgtg cagtgcacct ttattgcaag cacgattgat
     7201 cgatcagctg cccaacggta tgcggcgcgg ctttatgggt ttatcgacag acgctcagcg
     7261 ctgcctgcaa ttcgttcgat cgacccccgg tatggcggtg gcgctggccg gcatgagcca
     7321 agtggagcat gttgaagaaa atatgacgac cgctcaagtt gctccgctaa cattagagca
     7381 aattcaagcg ttgttgatac ggtaatatgc ctgaactctc tacggtcctc tcacccaaca
     7441 tcgcgctgaa aaaacttttt gatcatctgc cccgctcgag tgctattgaa gcgatcgaga
     7501 cggcaaacgg cttacagcgt gtgctggccg aatcgccgat cgcgacgatg cccctgcctt
     7561 catttccgcg cagcaccatg gatgggtatg cggtgcgcgc ggcagatacc ttcggcgcga
     7621 gtgaagcgct gcctgcttac ttgaaagtga tcggtgaaag tccgatgggc cgcgctccct
     7681 cgatcga
//
LOCUS       DASRTJ010000752         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_180486,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000752 DASRTJ010000000
VERSION     DASRTJ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180486"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFF70_15195"
     CDS             complement(<1..529)
                     /locus_tag="VFF70_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZY46083.1"
                     /translation="MNERRSDPIGVFICGTLSRMPPDLILRPVRLEDKPRVLEFTAHT
                     WGEDEDDYIQFVFDDWLYDPRGEFTAVESNGQCVAIAKLTDIGSNEWWFEGLRVDPEA
                     RGQGIGEELNRYQVDLARKLGGKTIRYMTASDNVGSLVIGSKAGFEHVLTYLMHTATP
                     LDQRYPRVRLTLDDLP"
     gene            complement(637..>1819)
                     /locus_tag="VFF70_15200"
     CDS             complement(637..>1819)
                     /locus_tag="VFF70_15200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZY46084.1"
                     /translation="RGETQVLSMATLGTLKDAQMLDGLDVDTTKRYMHHYNFPPYSTG
                     EARPMRGSSRREIGHGALAERALIPVLPSKEEFPYTIRVVSEALSSNGSTSMGSVCGP
                     TLSLMDAGVPIKAPVGGIAMGLISDGKRFSILTDIQGVEDHLGDMDFKVAGTLKGITA
                     LQMDIKIKGLSKEILEKALAQAREARIKVLDVLKSAIAAPRAELKPHAPRITTIHINP
                     EKIGAVIGPGGKMIRSIQEETGAQIDIEEDGTIYIASVEAAGAEAARLKIEALTESVE
                     IGKIYTGKVVRTTDFGAFVEIMPGTDGLVHISQLADFRVDTVESVVKLGDEIMVMVTD
                     IDPAGKIRLSRRAVLEGLTPEQAREMDKPRSSSGGDRGRSGGGDRRGGGGDRRGGGNR
                     H"
BASE COUNT          393 a          531 c          481 g          414 t
ORIGIN      
        1 cgggcaaatc gtcgagcgtg agacgaactc tggggtagcg ctgatcaagc ggagtagcgg
       61 tatgcatcaa ataagtgagc acatgctcaa agcctgcttt cgatccgatc accaggctgc
      121 cgacattgtc gctggccgtc atatatcgaa tcgttttgcc gccgagtttg cgcgccagat
      181 cgacttgata gcgattcagc tcttcaccga taccttgacc acgcgcctcc ggatcgacgc
      241 gcaggccttc gaaccaccat tcgttcgatc caatgtcggt gagtttagcg atggcgacac
      301 actgtccatt cgactcgaca gccgtgaatt cgccacgtgg atcatatagc caatcgtcga
      361 agacaaattg aatatagtcg tcttcatctt cgccccaggt gtgtgcggtg aattcgagaa
      421 cgcgcggttt gtcttcaaga cgaaccggac gaaggatcaa atcaggtggc atgcgcgaaa
      481 gtgtaccaca aataaaaacg ccgatcggat ccgatcggcg ttcattcatc aactgtcatt
      541 ccgaggcgcg tagcgctgag gaatctctac cactgattaa gattagtctc aacagcaata
      601 gagattcttc gctgcgctca gaatgacaaa aggttactaa tgtcggttgc cgccaccgcg
      661 gcgatcaccg ccgccgccgc gtcgatcacc acccccggaa cgacctcgat cgccaccgct
      721 gctcgatcgg ggtttatcca tctcgcgggc ttgttcaggc gtcaggcctt ccaagactgc
      781 gcggcggctt aatcgaatct tgccggccgg atcgatatcg gtcaccatga ccatgatctc
      841 atcgccaagt ttgacgacgg attcaactgt gtctacgcgg aagtcggcca gttggctgat
      901 gtgcaccagg ccgtcggtgc caggcatgat ttcgacaaac gcgccgaagt cggtggtgcg
      961 cacgactttg ccggtataaa tttttccaat ctcaaccgat tcagtcaacg cttcgatctt
     1021 caagcgcgcc gcttccgcgc cggcggcttc gactgaggcg atgtagatgg tgccatcttc
     1081 ttcgatatcg atctgcgcgc cggtctcttc ttgaatcgat cggatcatct tgccacccgg
     1141 gccgataacc gcgccgatct tttcgggatt gatgtgaatg gtggtgatac gcggcgcgtg
     1201 cggtttgagt tccgcgcggg gtgcagcgat ggcactcttc aatacatcca gaaccttgat
     1261 gcgcgcctca cgcgcttgcg ccaaagcctt ctccaaaatt tctttggaca ggcccttgat
     1321 tttgatatcc atttgcaacg ccgtgatgcc tttcaaagta ccggccactt tgaaatccat
     1381 atcgccgagg tgatcttcaa cgccttgaat atcggttaag atcgaaaagc gcttgccatc
     1441 actaatcaag cccatggcga tgccgccgac cggcgctttg atcggcacac ccgcatccat
     1501 caacgacaag gtcggtccgc agaccgagcc catcgaagtc gatccgttcg aggacaaagc
     1561 ttcactcacc acgcggatcg tgtaaggaaa ttcttccttg cttggcagaa ccggaataag
     1621 cgcgcgctca gctaatgcgc cgtggccgat ctcgcgtcga gacgatcccc gcataggcct
     1681 ggcctcgccg gtggaatatg gcgggaagtt gtagtgatgc atgtaacgct tggtcgtatc
     1741 cacgtcaagg ccgtccagca tttgagcgtc cttcaacgtg cccagcgtgg ccatcgacag
     1801 cacttgcgtt tcgccgcgc
//
LOCUS       DASRTJ010000753         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_221850,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000753 DASRTJ010000000
VERSION     DASRTJ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221850"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFF70_15205"
     CDS             complement(<1..194)
                     /locus_tag="VFF70_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HZY46085.1"
                     /translation="MRHRLAGKQLNRNTSQRLALRRGLVTELFRHGAIQTTQAKAAAI
                     RGMAEKLITTAKRSIVADDP"
     gene            complement(209..1207)
                     /locus_tag="VFF70_15210"
     CDS             complement(209..1207)
                     /locus_tag="VFF70_15210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZY46086.1"
                     /translation="MSSISMVLPKIETDATSKNYGRYIIGPLERGYGITLGNALRRVL
                     LSSLDGAAVTSVRLSDVHHEFSDVPHVREDMTQVILQIKQLRVKLHGEEPVRLRLEVR
                     GEGIVTAADIQCPPEVEVINPDLYLFTVDSDKARLEMELTVEPGRGYSPSDERGRLPI
                     GELPVDAIFSPVRRCNYDVERARVGQMTNYDKLLIEIWTDGTLRPQEALSHAAKILMQ
                     HFQLVSGVTEEIRMEEPAQSTSSIPQEIYETPIEQLDLSVRVFNSLKRTGITNVGEVL
                     EMLGRGEEAMLTIRNFGAKSLSELREKLIAKNFMKVGDLPDSLVASLPAETDEVEE"
     gene            complement(1306..1944)
                     /gene="rpsD"
                     /locus_tag="VFF70_15215"
     CDS             complement(1306..1944)
                     /gene="rpsD"
                     /locus_tag="VFF70_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZY46087.1"
                     /translation="MARTIGPVCKLCRREGEKLFLKGARCMSPKCAIERRGYAPGQHG
                     KTGQFKRGRSTDYLNQLREKQKARRIYGVLEKQFRRYFQIAQRSKGLTGAMLLTLLES
                     RLDNVVYRMGFATSRAQARQLVQHGHFDVNGRRTDAPSYLAQAGDMIAVHAGSRTGTY
                     FKEMPKVMEDYNAPKWLSVEPSQFGGKVLNLPMREEIDLSLNEQLIVEYYSR"
     gene            complement(2076..2477)
                     /gene="rpsK"
                     /locus_tag="VFF70_15220"
     CDS             complement(2076..2477)
                     /gene="rpsK"
                     /locus_tag="VFF70_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZY46088.1"
                     /translation="MGRSQRVKRTSPAKKQKRVSSHGHAHIHSSFNNTIVTITDTTGN
                     AIAWSSAGQAGFKGSRKSTPYAARLAGTNAAKTAVDMGVLEVDVFVKGPGPGREAAIR
                     ALQATGLRVRSITDVTPIPHNGCRPPKKRRV"
     gene            complement(2545..2934)
                     /gene="rpsM"
                     /locus_tag="VFF70_15225"
     CDS             complement(2545..2934)
                     /gene="rpsM"
                     /locus_tag="VFF70_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZY46089.1"
                     /translation="MARIAGVDLPRTKRVEVALTYIYGIGLKTSREVIALSRVNPDTR
                     VKDLTEAEVSVLRDLVEKQHKVEGDLRREVSLNIKRLVEIGAYRGLRHRRNLPARGQR
                     SRTNARTRRGPKKTVAGRGRRRGAKKK"
     gene            complement(2947..3060)
                     /gene="rpmJ"
                     /locus_tag="VFF70_15230"
     CDS             complement(2947..3060)
                     /gene="rpmJ"
                     /locus_tag="VFF70_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZY46090.1"
                     /translation="MKVSPSVRPICPKCKLIRRKGKLRVICENPKHKQVQG"
     gene            complement(3086..3751)
                     /locus_tag="VFF70_15235"
     CDS             complement(3086..3751)
                     /locus_tag="VFF70_15235"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZY46091.1"
                     /translation="MADYYLLFGPPGVGKGTQADHLEKTLDLAHVATGDLFREHLKKN
                     TPLGQLARSYIDRGALVPDNVTIGMVAERMTEPDTQQGMLLDGFPRTIAQADALSKML
                     ADRGQKIQRVLFITAPKDILIERLGHRRTCSNCGTIYNAQTQLPKNARVCDVCGGEVI
                     QRSDDTPDTQRKRIDVYLEQTIPLVDYFAQRGLLVTIQGEQSIDRVYEDLLQAIETAK
                     NIG"
     gene            complement(3796..>4837)
                     /gene="secY"
                     /locus_tag="VFF70_15240"
     CDS             complement(3796..>4837)
                     /gene="secY"
                     /locus_tag="VFF70_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZY46092.1"
                     /translation="VIPALGRIMKEEGSAGRKKIETYTYILTVPMAALSAIGQIRIFE
                     SGGSPIIKNFGVDGLATLSIIVTMTAGTMFAIWLGELITAQGVGQGISIIIFGGIVSR
                     LPQNIGQLLADPSRAAGSLIAFAIIVILIVVVIVFIQEGERRIPVQYGKRVRGRRVFG
                     GGSTHIPLKVNMAGMIPLIFAQSILTLPAILAQFLANSPDATVANIFGSIRDIFSGGR
                     NSASFLYWFFMFFLVVGFTYFYTDVMVQQQNLPDSLQKQGGYIPGIRPGARTEEYLNG
                     VTRRITLVGALFLGGIAIVPFLISLTGLIENATNFSILSTGLLIVVGVVLDTIRQLEA
                     QLTMRHYEGFIK"
BASE COUNT         1086 a         1463 c         1190 g         1098 t
ORIGIN      
        1 atgggatcgt cggcgacgat gctgcgcttg gcggtcgtga tcaacttctc ggccatgccg
       61 cgaatcgctg cggctttcgc ctgcgtcgtt tgaatcgctc cgtggcggaa aagttccgtg
      121 actaagccgc ggcgcaatgc cagccgctgc gaagtgtttc gattaagttg tttacccgct
      181 aaacgatggc gcatgtctta gcatcctttt actcttcgac ttcgtcggtc tcagccggca
      241 aactcgcgac caaactatcc ggcagatcgc cgaccttcat gaagtttttg gcgattagtt
      301 tctcgcgtag ttcggacagc gatttcgcac caaagttgcg aatggtcaac atggcttctt
      361 cgccccggcc gagcatttcc agcacttcac cgacattggt aatgccggtg cgcttcagtg
      421 agttgaagac gcggaccgat aagtccagct gctcgatcgg ggtttcgtag atctcttgcg
      481 ggatcgacga agtcgattga gccggctctt ccattcggat ttcttcggtg acgccggaga
      541 ccaactggaa gtgctgcatc aatattttcg ccgcgtgact caaagcctcc tggggtctga
      601 gcgttccatc ggtccagatc tcgatcaaca gcttatcgta gttggtcatc tggccgacac
      661 gcgcgcgttc tacatcatag ttacaacggc gcaccggact gaaaatcgcg tcaaccggca
      721 attcaccaat cggcaatcgg ccgcgttcat cggacggact ataaccacgg cccggctcaa
      781 cagtcagttc catttccagt cgagctttgt cactatcgac cgtaaagaga taaagatcgg
      841 gattgataac ttcgacttcc ggcgggcact gaatatccgc agccgtcacg ataccttcac
      901 cgcgcacttc caagcgcagg cggactggct cttcgccatg caacttcacc cgcaactgtt
      961 tgatctgcaa gatgacttgc gtcatatcct cgcgcacgtg cggcacatcc gaaaattcgt
     1021 gatggacatc actcagacgc accgaggtta ccgctgcacc atccaaactg ctcaacaata
     1081 cccggcgcaa ggcattgccg agtgtaatcc cataaccgcg ctccaacggg ccgatgatat
     1141 accggccgta gttcttggac gtggcatcgg tttcgatctt gggcaagacc atactaatac
     1201 ttgacaagga atcctcctcg gatcacggct gaaccatttg gcctaccgct cagccgcacc
     1261 cagcgggcga tcgatccgat cgcccatgca tgttatcgat aagctttagc gcgagtagta
     1321 ctcgacgatc agctgctcat tgagcgacag atcgatttct tcgcgcatcg gcagattgag
     1381 caccttacca ccgaattgac taggctcaac actcaaccac ttgggcgcgt tgtaatcttc
     1441 catcactttc ggcatctctt tgaagtaagt gccggtgcgg ctgcccgcat ggaccgcaat
     1501 catatcgccg gcctgcgcca aataggatgg cgcatcggtg cggcgaccgt tcacatcgaa
     1561 gtgaccgtgc tgtaccaatt gacgtgcttg cgcccgcgag gtggcaaagc ccattcgata
     1621 cacgacatta tccaagcggc tttctagcag cgtcaacaac attgcgccag tcaagccctt
     1681 gctgcgctgg gcaatttgga aatagcgacg gaactgcttc tccaacacgc cgtaaatacg
     1741 gcgcgccttc tgcttttcgc gcaattgatt taaatagtct gttgaccgac cgcgcttgaa
     1801 ctgtccagtc ttgccgtgct gacccggcgc gtagccgcgt cgttcgatcg cacacttggg
     1861 tgacatgcag cgcgcaccct tcaggaacag tttctcgcct tcgcgtcggc acagtttaca
     1921 cacaggccct attgttcttg ccatgcttct tcctgtcgcc tttctctaag aatttttctt
     1981 catccgttga gaatgaagtt gctcacattc aaaaatcaat atgctgtaac tggtggggtg
     2041 cagatgaggc gcgaacccac ccgcacacgg atcaactaca ctcgccgttt cttcggcggg
     2101 cggcaaccat tgtgcggaat cggggtcacg tcggtgatcg atcggacccg cagtccagtc
     2161 gcctgcaagg cgcgaatcgc cgcctcgcgg ccggggccag gccccttcac aaacacatcc
     2221 acttccagta cacccatatc gaccgcggtt ttggctgcat tcgtgccggc caatcgagcg
     2281 gcgtacggcg tgctcttgcg actgcccttg aagcctgcct gtcctgcgct gctccaggcg
     2341 atcgcattgc ccgttgtatc ggtgatggtc acgatcgtgt tgttgaagct cgaatggata
     2401 tgagcatgac cgtgcgacga cacacgcttt tgcttcttag ccggtgatgt ccgtttaacg
     2461 cgttgacttc gacccatgaa cgttctcctc actcatcgga ttcgcaaccc gcatcgattg
     2521 agctgcgaac cagcactcac tgctttactt cttcttagcg ccacgacgtc gaccacggcc
     2581 agccaccgtc ttcttgggac cacggcgggt acgcgcattg gtgcgtgatc gctgaccacg
     2641 cgccggcaga ttgcggcgat gccgcagacc gcgatatgcc ccgatttcga ccaagcgctt
     2701 gatgttcaac gacacctcgc gccgcagatc gccttcgacc ttgtgctgct tctcgaccag
     2761 atcacgcagc acactgactt ctgcctcggt caaatccttc acccgcgtat cgggattgac
     2821 ccgcgacagc gcgatgactt cccggctggt ctttaagcca ataccataga tgtaagttaa
     2881 tgcgacctca acccgcttcg tgcggggcag gtcaacgccc gcaatacgcg ccatagaatt
     2941 cctccgctaa ccttgcactt gcttgtgctt gggattttcg caaatcacgc gcagtttccc
     3001 tttgcgccga atcaatttgc acttcggaca aatcggccga accgatggtg aaactttcat
     3061 ctggataact cctattcagt caaacttaac cgatattttt tgccgtttcg atcgcctgca
     3121 ggagatcttc gtaaacccga tcgatcgact gctcgccttg aatcgtcact aacaaaccgc
     3181 gttgcgcaaa ataatccacc agcggtatcg tctgctcaag atacacatcg atgcgtttcc
     3241 gttgagtatc cggggtatca tcggagcgct gaatcacttc accgccgcac acatcacaca
     3301 cgcgagcgtt tttgggcaac tgtgtctgcg cattataaat cgtaccacag ttcgaacaag
     3361 tcctgcgatg tcccaaccgc tcgatcagaa tatctttggg cgcagtgata aacagcacac
     3421 gctgaatctt ctgcccccga tcagctaaca tcttgctgag cgcatcggct tgggcgattg
     3481 tccgcggaaa accatccagc agcatgccct gctgcgtgtc aggttcagtc atccgttcgg
     3541 cgaccatgcc gatcgtcaca ttatccggca ccaatgcgcc gcgatcgata taactccgcg
     3601 ccaattgacc taaaggcgta ttcttcttca aatgttcgcg aaacagatcg ccagttgcaa
     3661 cgtgagccag atctaacgtc ttctccaggt gatcggcttg agtgccttta ccaactccgg
     3721 gcgggccaaa gagcaaataa tagtctgcca tttaccacca gacctccatc ccccgccaac
     3781 ccccgccgct agaatctatt tgataaagcc ctcataatgg cgcatcgtta attgcgcctc
     3841 caattggcga atggtatcca gcaccacacc gacgacgatc aacaagcctg tacttagaat
     3901 tgaaaagttc gtcgcgttct cgatcaagcc ggtgagcgaa atcaaaaacg gcacgatggc
     3961 aattccgccc aagaacaacg cacccaccag cgtgatgcgg cgcgtcacac cattcaggta
     4021 ttcctcggtg cgcgcgcccg gacggatgcc cggaatgtaa ccgccctgct tctgcaatga
     4081 atcgggcagg ttctgctgct gcaccatcac atcggtgtag aagtacgtaa agcccacgac
     4141 caggaaaaac ataaagaacc aatacaggaa cgaggccgaa ttccgtccgc cggaaaatat
     4201 atcgcgtata ctgccaaaga tattggcgac ggtggcatct ggcgagttcg ccaagaactg
     4261 cgccaagatt gcgggcagag tcagaatcga ttgtgcgaag atcaacggaa tcatgccggc
     4321 catgttaact ttcagcggaa tgtgagtcga tccgcccccg aatacgcgcc gcccgcgcac
     4381 gcgttttcca tattgcaccg gaatacgccg ctcaccttcc tgaatgaaga caatcaccac
     4441 cacgatcaat atcacaatga tggcaaaggc aatcaatgaa ccggctgcgc gcgaaggatc
     4501 cgctaacaac tgtccgatgt tctggggcaa gcgcgacacg atgccgccaa agattataat
     4561 cgagatgcct tgcccgacac cctgcgccgt aatcagctcg ccgagccaaa tggcaaacat
     4621 ggtaccggcc gtcatcgtca caatgatcga cagtgtcgct aacccgtcca caccaaagtt
     4681 cttgatgatg ggtgagccgc ccgattcgaa aatccggatt tgcccgatgg ccgataacgc
     4741 cgccatcggc actgttaaga tataggtgta agtttcaatc ttcttgcggc cagctgagcc
     4801 ttcttccttc atgatacgcc caagcgctgg aatgacg
//
LOCUS       DASRTJ010000754         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_180747,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000754 DASRTJ010000000
VERSION     DASRTJ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180747"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFF70_15245"
     CDS             complement(<1..689)
                     /locus_tag="VFF70_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46093.1"
                     /translation="MKIIVCIKQILDPRGLTVNRKAEKVFVNREEYLIDPASKAALEV
                     AESIKGLNAEVIAISIGPARVDEALREALARGADRSILIRSDVPLDAFVVTNLLAAAI
                     DKIGEVDLVLCGDRSLDTACGEIAPRLAEALNLPSILNAAKIEIKDQLLTATVHQSGF
                     LSVESKLPVVVSFTAEAFTGKFANAWRLVDAYKKWDVETWSISDLGLSEEDLQPLTVK
                     KEDAFPPERQFG"
     gene            complement(785..1966)
                     /locus_tag="VFF70_15250"
     CDS             complement(785..1966)
                     /locus_tag="VFF70_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY46094.1"
                     /translation="MDFELSREHEAFRKLMREFAEEEVRPVARPTDRTGEPALAVIKK
                     AGEIGLLGAPYPREYGGLGLGETGLCVLMEELGKVDTSVSTMIGAHSGIGTAPIYLAG
                     SQAIKEKYLPDLCSGKKIAAFALTEPQAGSDAASIKMRATREGDSFILDGSKMWITNG
                     PIANVMSILAVTDPTLGARGGATAFVIDTRHNGSLADGFTPGKPEHKLGLHSSPTSTL
                     TFDNFRVPRENTLGQEGLGFVVMMQTLDLGRVSLGAATLGGAQAALQQSLEYAALREQ
                     FGRPLAHNQSIQFMIADMAAEVEALRSLVYRTAWLADSGKPFTKEAGICKLLGSEMAS
                     RCIDRAVQIHGALGYSRDFNIERGYRDGRITEIFEGTNEIQRILIAEQMFREVGVKIR
                     P"
     gene            complement(1998..2600)
                     /locus_tag="VFF70_15255"
     CDS             complement(1998..2600)
                     /locus_tag="VFF70_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46095.1"
                     /translation="MKQTIRVVSFVVSQIVNVPFVAGAAITLLLFGLRPDVPQRILGW
                     GVSLIFLTVIPALSLLFYIPRRNEPWSSTEKRQRVMSFVFMAVSYPIGFVILKIIQVP
                     TIFEAIMLTYVVVVLALILVNLFFKASGHAAGVAGPVSALIYFYGFIATPLIALIPLT
                     MWARVRSKGHTLSQTIIGALLSAGVTIAILFLYGFRPGQL"
     gene            complement(2609..3751)
                     /locus_tag="VFF70_15260"
     CDS             complement(2609..3751)
                     /locus_tag="VFF70_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZY46096.1"
                     /translation="MDFNLTADQKMFRDLFRDFATKEVAKVAKHIDETEEPPIDLLKK
                     AAAQGFLAATIPEELGGAALDPLSYALLIEEIGKVCASTAITLALHNSLAVMPLILFG
                     TADQKQRWAPKLAESIGAFALTEPEAGTDANHLSTRARKDGDSYVIDGVKTWVSNAGI
                     ASTLTFIADTANGPLAFIADTKTPGIKIGHREPTMGLRGVSINTVYFDHAVMPVSDRL
                     GSEGEGRHVAHEALAHMRLAIAALALGAAEGALALGRTFAIERKQFGVSIATKQAIGN
                     YFADTEIDIEALRHLIEYTAWLIEQKKDYKQAALKAKTLGAKVAREAANRMLQVHGGY
                     GFSDEYAISRVYRDVRALDIMGGTPQIARVLIAREIFEESGVKIKP"
     gene            complement(3844..>4114)
                     /locus_tag="VFF70_15265"
     CDS             complement(3844..>4114)
                     /locus_tag="VFF70_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZY46097.1"
                     /translation="QAMQLRLVNMVVPGGEVMRQSIGLAKKFADKSSVGITAILGSIR
                     EGLDADLQSGLAAERKNFVNVASSADAQEGIMAFLEKREAKFQDK"
BASE COUNT          926 a         1233 c         1035 g          920 t
ORIGIN      
        1 ccaaattgtc tttccggcgg gaaagcatct tccttcttta cagtcaacgg ctgcaaatcc
       61 tcttcgctta atcccaaatc tgatatgctc caagtctcaa cgtcccattt cttgtaagca
      121 tcgaccaagc gccaagcatt ggcaaacttt ccggtgaagg cttcggccgt aaacgaaaca
      181 accaccggaa gttttgattc aacgctgagg aaaccggatt gatgcactgt cgccgttaac
      241 aactgatctt tgatttctat tttggccgcg tttaagatcg agggcaagtt caacgcttcg
      301 gccaggcgtg gagcgatctc gccgcacgcg gtgtccaatg atcgatcgcc gcataagacc
      361 agatcgactt cgccaatctt atcgatcgcc gccgcgagca aattagtgac gacaaatgca
      421 tcgagcggaa cgtccgatcg gatcaaaatt gatcgatcgg cgccgcgagc cagtgcttca
      481 cgcagggctt cgtcgacgcg cgcagggccg atcgagatcg cgatcacttc agcgtttaag
      541 cccttaatcg attcagcgac ttccaacgcc gccttgctgg ccggatcgat cagatactcc
      601 tcgcgattga cgaaaacttt ctcggccttg cgattgacgg tgaggccgcg cggatcgaga
      661 atttgtttga tacagacgat gatcttcatg gagcgatcct tattaaccac ggattacaca
      721 gatgacaagg atattttgct tttgcgttta atcagtgaaa tccgtgtcat ctgtggttta
      781 ttttttatgg gcgaatcttc acgcccactt ctctaaacat ctgctcggca atcaagatgc
      841 gttgaatctc attcgtgcct tcaaagattt cggtgatgcg gccatctcga taaccgcgct
      901 cgatgttgaa gtcgcgactg taaccaagcg cgccatgaat ctgcaccgcg cgatcgatac
      961 agcgtgaagc catctccgag ccgagcagtt tgcagatgcc cgcttcttta gtgaacggtt
     1021 tgccactatc agccagccag gcggtgcgat acaccaacga tcgcagggct tcgacttcag
     1081 cggccatgtc ggcgatcatg aattgaatgg attgattgtg cgccagtggt cggccaaatt
     1141 gttcgcgcaa cgcggcatat tccagtgatt gttgtaacgc ggcttgcgcg ccgcctaatg
     1201 ttgctgcgcc gagcgacacg cggcccagat cgagcgtctg catcatcacc acaaaaccca
     1261 agccttcttg acccaaagta ttctcgcgcg gtacgcggaa attatcgaag gtcaaggtgg
     1321 atgtcggcga actatgcaga cccagtttgt gttcgggctt gccaggtgta aagccgtccg
     1381 ccaatgagcc gttgtggcgc gtatcgatca cgaacgcggt cgcgccgccg cgcgcgccca
     1441 aggtcggatc ggtcaccgcg aggatgctca tcacattcgc gatcggcccg ttggtgatcc
     1501 acatcttcga gccgtcgaga atgaaggagt cgccttcacg cgtcgcgcgc attttgatcg
     1561 aggccgcatc cgatccggct tgcggttcgg tcaacgcaaa cgctgcgatc tttttgccgc
     1621 tgcacagatc gggcagatat ttttctttga tcgcctggct gccggccaaa tagatcggcg
     1681 cggtgccaat gccgctgtgt gcgccgatca tcgtcgagac actcgtgtcg accttgccga
     1741 gttcttccat caagacgcac aggccagttt cacccaggcc gagtccgcca tactcacgtg
     1801 gatagggcgc gccgagcaga ccgatctcgc ccgctttctt gatcacagcc agtgcgggct
     1861 cgcccgtccg atcggtgggg cgcgcgacag gacgaacttc ttcctcggca aattcgcgca
     1921 tcaattttcg gaaggcttca tgttcgcggc taagttcgaa atccatagtg ttacctcaac
     1981 gattggtcat cagcaattta taactgcccc ggacgaaaac catacaagaa caagatcgca
     2041 atcgtcacgc ccgccgacaa caacgcgccg atgatcgttt gcgacagcgt gtgacccttc
     2101 gatcgaacgc gcgcccacat cgtcaacgga atcagcgcaa tcaacggcgt ggcgataaaa
     2161 ccgtaaaaat agatcaacgc gcttaccggc ccggcaacgc ccgcagcgtg accgctcgct
     2221 ttgaaaaaca gattcaccag aatcaacgcc agcacgacga cgacgtacgt cagcatgatc
     2281 gcttcaaaga ttgttggcac ttggatgatc ttcaagatca caaacccgat cggataactg
     2341 actgccatga aaacgaaact catcacgcgc tgacgtttct ccgtggacga ccacggctcg
     2401 ttacggcgcg gaatgtagaa caacaaactc aaagccggaa tcacggtcaa gaagatcaat
     2461 gagacgcccc aaccgagaat gcgctgcggc acatcgggcc gcagaccaaa cagcaacagc
     2521 gtgatcgccg cacccgcgac gaacggcaca ttcacaattt gcgacacaac aaaactgaca
     2581 acccgaattg tttgcttcac acgtcccgtc atggcttgat cttcactccc gattcttcaa
     2641 atatttcacg cgcgatcaac acccgcgcga tctgcggtgt gccgcccatg atgtccaacg
     2701 cacgcacgtc gcgataaaca cgcgagatgg catactcatc gctgaaacca tagccgccgt
     2761 gtacttgcag catacgatta gccgcttcgc gcgccacttt cgcaccgagc gttttcgctt
     2821 tcagcgcggc ttgtttgtaa tctttcttct gctcgatcaa ccacgcggta tattcgatca
     2881 agtgccgcag cgcttcgata tcgatctcgg tatcggcaaa ataatttcca atcgcttgtt
     2941 tcgtggcgat gctgacgccg aactgtttgc gctcgatcgc gaaggtgcgg cccaacgcga
     3001 gtgcgccttc ggctgcgcct aacgctaacg cggcgatcgc caagcgcata tgcgccaaag
     3061 cttcgtgagc gacgtgtcgc ccttcgcctt cgctgcccaa acgatcagag acaggcatga
     3121 ccgcgtgatc gaaataaacg gtattgatcg aaacgccgcg caagcccatc gtcggttctc
     3181 gatggccgat cttaatccct ggcgtttttg tatccgcgat aaacgccaac ggaccattag
     3241 cagtatcggc gatgaaggtc agcgtcgaag cgatccccgc attactaacc caagtcttca
     3301 cgccatcgat cacgtagcta tcgccatctt tgcgtgcgcg ggtcgataaa tgattcgcgt
     3361 ccgtgcccgc ttccggttcg gtcaacgcaa acgcgccgat cgattctgcg agtttcggcg
     3421 cccaacgctg tttctgatca gcggtgccga acaaaatcag cggcatcacc gcgagactat
     3481 tgtgcagcgc caacgtgatc gccgtcgatg cgcacacttt gccaatctct tcgatcagca
     3541 gcgcatagct cagcgggtcc agcgccgcgc cgccgagttc ttctggaatc gtcgccgcta
     3601 aaaagccctg tgcagccgcc ttcttcagca aatcgatcgg cggctcttcg gtctcatcga
     3661 tgtgcttcgc caccttcgcc acttctttcg tcgcgaagtc gcggaacaaa tcgcggaaca
     3721 ttttttgatc ggcggttaaa ttgaagtcca tatgtaactc ctagaccgaa gacggcagac
     3781 tgaagaccgc cgttgatgtt gatcaacatg gatcatcttc tgtcttcggt catcggtcag
     3841 cggtcatttg tcttggaact tcgcctcgcg cttttccaag aacgccatga taccttcttg
     3901 cgcatccgcg ctcgaagcga cgttgacaaa gttcttgcgt tccgcggcca agcccgattg
     3961 caaatccgca tcaagtcctt cgcggatcga tccgaggatc gccgtgatgc cgaccgagct
     4021 cttatccgcg aacttcttcg ccaacccgat cgattgacgc atcacttcgc cacccggcac
     4081 gaccatgttc accaagcgca gctgcatcgc ttgc
//
LOCUS       DASRTJ010000755         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_222163,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000755 DASRTJ010000000
VERSION     DASRTJ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222163"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFF70_15270"
     CDS             complement(<1..552)
                     /locus_tag="VFF70_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZY46098.1"
                     /translation="MLKDAPTLTTEELVAKAKKPSADAMRLHPFYHGKMQTALRCRVR
                     DFNDFAIWYTPGVAAPCKAIQANPELVYEMTNKWNTVAVVSDGTRVLGLGDIGPKAGL
                     PVMEGKALLYKYLGGVDAWPIMLDTKDPDKIIDTVLMLQPGFGGVNLEDLSQPKCFRI
                     LDTLREQAEIPIWHDDQQGTATVT"
     gene            1043..>1639
                     /locus_tag="VFF70_15275"
     CDS             1043..>1639
                     /locus_tag="VFF70_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZY46099.1"
                     /translation="MALASLLILFLVVPLIVILLRAIEAGLLDQLTQPTVSQALQLSL
                     ITSFFSTLIAIIFGTPLAYILARRRVRARRVIETLLDLPIVLPPAVAGLALLMTFGRR
                     GLFGSALDSIGLSLPFTSIAVVLAQMFVAGPFFIRSAQTGFASVSREMEEVASDLGAN
                     NWLVFKRVTLPLAAPSLLAGIILTWARALGEFGATILFA"
BASE COUNT          337 a          444 c          442 g          416 t
ORIGIN      
        1 cgtcaccgtc gcggtgccct gttgatcgtc atgccagatc ggaatctccg cttgctcgcg
       61 cagcgtatcg agaatgcgga agcacttggg ctgtgacagg tcttcgagat ttacgccgcc
      121 gaaaccgggc tgcagcatca agaccgtgtc gatgatcttg tccgggtctt tggtgtcgag
      181 catgatcggc cacgcatcca caccgcccag atatttgtac aacagcgctt tgccttccat
      241 cacgggcagg ccggctttgg ggccgatgtc gcccagcccg agcacccgcg tgccgtctga
      301 cacgacggct acggtgttcc acttgttcgt catctcgtac acgagttccg gattagcctg
      361 aatcgctttg cagggcgcgg ccacaccggg cgtataccag atcgcaaaat cgttgaagtc
      421 acgcacacgg caacgcaatg cagtctgcat cttgccgtga taaaacggat gcaagcgcat
      481 ggcatccgcc gagggcttct tcgctttcgc taccaactct tcggttgtta aagtcggtgc
      541 atctttgagc atatactttc ctccactgaa taatgggttg tcaattccgg taaaggcgct
      601 cgaacgatcg acattgagtg cagctataac cttcgctcag atcgccgttt gcgcctagtg
      661 ttggatgtca ttgaattgtg agaaattcat caaacgcgtg ttaatttgtg aattgaaaag
      721 atggcgatgg tcaaaaataa aagccgctta ttccacaaaa tgaacaagcg gctaaaaata
      781 tctgtaggtg gggcaaccct atgcctcgcg aataaaatgg gtgattattt cgtcgccttg
      841 gcctcttcca atttcggcag tgtcagcatt tgctcaaagc ccgcgtccgc gcggttcgag
      901 agcgttttaa aatggaatgc tgtaaaaatg tcagacgtgc gccgcccctt ttatttcagg
      961 tatacttctg tcacgagcca tgatccattt cctaaaaaca aaacttcaaa acattgcaat
     1021 caaatttggc cgaatatggg tgatggcgct cgcatcactg ctgatcctgt ttctggtcgt
     1081 gccgctgatc gtcatcttac tgagggcgat cgaggccggc ctgctcgatc aattgactca
     1141 gccgacggtc agccaggccc tgcaattgag cttgatcacc agcttcttca gcacgttaat
     1201 cgccatcatc tttggcacgc cgctggctta tatcttggcg cggcggcggg tgcgggcgcg
     1261 gcgcgtgatt gaaacgctgc tcgatctacc gatcgttttg ccgcccgctg tcgcaggttt
     1321 ggcgctgctc atgaccttcg gtcgacgcgg gctcttcgga tcggcgctgg actcgatcgg
     1381 attgagcttg ccctttacgt cgatcgccgt ggtgctggcg cagatgttcg tcgccgggcc
     1441 gttcttcatt cgatcggctc aaacgggctt tgccagcgta tcgcgagaga tggaagaagt
     1501 tgccagcgat cttggcgcaa acaattggct agttttcaaa cgtgtgacct tgccgctggc
     1561 cgcaccctct ttattagccg gaataatttt gacgtgggca cgcgccttag gcgaattcgg
     1621 ggcgacgatt ttgtttgcg
//
LOCUS       DASRTJ010000756         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_222177,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000756 DASRTJ010000000
VERSION     DASRTJ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222177"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFF70_15280"
     CDS             complement(<1..154)
                     /locus_tag="VFF70_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZY46100.1"
                     /translation="MRLLIATTNPGKVKEFQSLFEGLNLELVGLTDLGITTEVEETGS
                     TFAENAE"
     gene            459..890
                     /locus_tag="VFF70_15285"
     CDS             459..890
                     /locus_tag="VFF70_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZY46101.1"
                     /translation="MGLEINEVDQTLGDLKLDFVRGHLRLTRANRRILIKGKLHTVVD
                     AECVRCLTPFRLALNLQLEEMFALSSSVDPVYFVNDDGWLNLQLPLREQVLLAMPIQL
                     FCRPDCKGLCPECGQNWNDGPCEHQGQEIDPRLAALKSLLH"
     gene            903..2102
                     /locus_tag="VFF70_15290"
     CDS             903..2102
                     /locus_tag="VFF70_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZY46102.1"
                     /translation="MANPADNKLIDLFSELMERAEAQGYLTTDDILETLPETDDLSER
                     LEELLAQLRDAGVDIYDSKVGIDAALLQVDDFDDEEESESASDDEDFELAAVNTDDTV
                     GLYLKEMARVPLLTSAEEVQLAKQVLRGRLAIKSLKRATRCQQLRRTKLEQDIDQARL
                     AREHLIKANTRLVVSIAKRYIGQGVPFLDLIQEGNIGLMKAVEKYDYTRGFRFSTYAT
                     WWIRQTVTRAIADQARTIRLPVYMSDLLRQLYQVARQIEQTNGKQATAEEIALELHME
                     PRRVQWLMKASWQPLSLEQPMGEDDDNELASFIEDDSTPSPAQLALDNLLHDKVEEVL
                     STLSPREASILRLRFGLENGHAYTLKEVGQKFGLTRERIRQIENQALRRLRHPRRSRQ
                     LRDYVNP"
     gene            2300..>2748
                     /locus_tag="VFF70_15295"
     CDS             2300..>2748
                     /locus_tag="VFF70_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZY46103.1"
                     /translation="MSDKILVSVAWPYANADIHVGNITGSHLPADIFARYHRLKGNDV
                     LMVSGTDSHGTPVTVRADAEKSTPLIVYEKFHRRFIELFVKLGITFDLYTSTHTENHF
                     KVSQDMFKALLDNGYLYREKQMLLYSESEKRFLPDRYVEGECYICHYP"
BASE COUNT          641 a          742 c          740 g          625 t
ORIGIN      
        1 attcggcgtt ttcggcaaag gtcgagccgg tttcttcgac ttcggtcgtg atgccgagat
       61 cggtcaggcc aactagctca aggttcagtc cttcaaatag cgactggaac tctttgactt
      121 tgcctggatt ggtggtcgcg atgagtaagc gcatgaaatt tccactaacc gatcggcttt
      181 gggaggccga tcgggtctaa tctagagcga atataacctc cgccaacgat ttctgcaagt
      241 tcgatcgagt taatatcggg ccagatcggc ttatgttacc cgattatccg caaacgtgtt
      301 aaatgcttga cgcccgatat tcaatatgct acaatatcgt catcgttaac ctgtcgtcac
      361 cactccatta tcagcctttt taggctgaat ccgccgccga attcacagtg ttgagatata
      421 acatctccct acttttaaaa cctgaagtag gcgccaccat gggtttggag ataaacgagg
      481 tcgatcaaac cctaggcgac ctcaagttgg atttcgtgcg cgggcattta cgcctcactc
      541 gcgcgaaccg ccggattctg atcaaaggca agctccacac ggtggtcgat gcagaatgtg
      601 tccggtgtct gacgcccttc cggctggctt tgaatttgca actggaagaa atgtttgcgc
      661 tgtcctcttc agttgatccg gtgtactttg tgaatgacga tggctggctg aacttgcagc
      721 tgccgctgcg cgagcaagtt ttgctggcga tgccgatcca gttgttctgc aggcccgatt
      781 gtaaaggctt gtgcccggaa tgcggacaga actggaacga cgggccgtgc gaacatcaag
      841 gccaggaaat tgatccacgt ttggcagcac tgaagtccct acttcactag cggaggtaag
      901 tgatggccaa tccggcggat aacaagctga tcgatctgtt tagcgaattg atggaacgcg
      961 ccgaggcgca gggttacctg accacggacg acatcctgga gacgctgccc gagaccgatg
     1021 atctcagcga acgcctcgaa gaactcctcg cgcaactccg cgatgccggc gtcgatatct
     1081 acgactcgaa agtcggcatc gatgcagcgc tgttgcaagt cgatgacttc gacgatgaag
     1141 aagagtcgga gtcggcctcg gacgatgaag acttcgaact cgccgcagtc aacacagatg
     1201 atacggtcgg actttatctg aaagagatgg ctcgcgtgcc cttgctgacc agcgctgaag
     1261 aagttcaact ggcgaagcaa gtcctgcgcg gacgcttggc cattaaatca ctcaagcgcg
     1321 ccacccgctg tcagcaattg cgccgcacca agctggagca ggacatcgat caggcccgct
     1381 tggcgcgcga gcatttgatc aaagccaata cccgcttggt ggtcagcatc gccaaacggt
     1441 atataggtca gggtgtgccg ttcctcgatc tgattcagga aggcaacatc ggcttgatga
     1501 aggccgtcga gaaatatgat tacacgcgcg gctttcggtt cagcacttac gccacctggt
     1561 ggattcgaca gaccgtcacg cgcgccatcg ccgatcaagc ccgcaccatc cgtctgcccg
     1621 tgtacatgag cgatctgctc cgccaactgt atcaagttgc tcgccagatc gagcagacca
     1681 acggcaaaca agcgaccgcc gaagagattg cgctggaact gcatatggag ccgcgcagag
     1741 tgcagtggct gatgaaggct tcctggcagc cgctctcgct agaacagccc atgggcgaag
     1801 atgacgataa tgaactggcg tcgttcatcg aagacgatag cacgccgtcg cctgcccagt
     1861 tggcgctgga taatttgctg catgacaagg tggaagaagt tttatcgacg ttgagtccgc
     1921 gcgaagcaag tattttacgg ctgcgcttcg gcttagagaa cggccatgct tacaccttga
     1981 aggaagtcgg ccagaaattc gggctgacgc gcgagcgcat tcgccagatc gaaaatcagg
     2041 cgctgcgccg cctgcgccat ccgcgccgat cgcgacaact gcgcgattac gtgaatccat
     2101 gaagatgaca cattttattt cgatgtccat gcgaagcgat ctaacacaat cgggcgatga
     2161 ggcagcccga tcggattgat cgcgctttgt tgatcgaaat ctcaaccgcc agcctcatgc
     2221 tggcggtttt gttttggtat gatagaatgt tgaacttagt gaaagttctg aaccttcgcc
     2281 gggttttgga gagtttataa tgtccgataa aatcctcgtt tccgtggcct ggccgtacgc
     2341 caacgccgat attcacgtcg gcaacatcac cggctcgcat ctgcccgccg atattttcgc
     2401 gcgctatcat cgcttgaagg gcaacgatgt gctgatggtc tccggcacgg actcgcatgg
     2461 cacgcccgtc accgtccgcg ccgatgccga gaaaagcacg ccgctgatcg tgtatgaaaa
     2521 atttcatcgc cgcttcattg agctgttcgt gaagctcggc atcacgttcg atctctacac
     2581 cagcacccac accgagaatc atttcaaagt ctcgcaggac atgttcaagg cgctgctcga
     2641 taacggttat ctgtatcgcg agaagcagat gctgctctat tctgaaagcg aaaaaagatt
     2701 tttaccagat cgctacgtcg agggtgaatg ctacatctgc cattatcc
//
LOCUS       DASRTJ010000757         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_222244,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000757 DASRTJ010000000
VERSION     DASRTJ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222244"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..429)
                     /locus_tag="VFF70_15300"
     CDS             complement(46..429)
                     /locus_tag="VFF70_15300"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZY46104.1"
                     /translation="MPRKNTAEIDELLKNWEEVYKKGLLTFWILLLLHDRPGYAYEMS
                     AAIGELSEGTITADENSIYRALNRFEPLGIVQSELKQSDVGPQRRYYRLTENGTALLA
                     KFVQRNVLVFEKPEIAERIQAIVTK"
     gene            complement(542..1894)
                     /locus_tag="VFF70_15305"
     CDS             complement(542..1894)
                     /locus_tag="VFF70_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZY46105.1"
                     /translation="MDFNSFDNYIAGHQEKFIDDLKAFLRQPSIAATGEGVHEMANLL
                     QGRLESIGAKVQQFPTGRAPIVYAELGSGPRTLMIYNHYDVQPADPIDLWTTPPFEPT
                     LRDGKLFARGVSDDKGDLVCRIQAIASWQKTIGALPLKIKWVIEGEEEVGSENLAEFA
                     EKHKDILKADGCLWEGGGKDEADRYVLSMGLKGIQYLELRVHGARRDLHSGYAAIVPS
                     PVWRLIWALNTIKDEHDNILIDGYYAHVRQPNEAELKMLRAMPFDETALKKMFGFDRF
                     IENETGLDLLKRFLYGPTATICGIHAGYTGEGMKTVLPNYAFAKMDFRLVPNLTPELC
                     LSLLRAHLDRRGFTDVEIVPMSAEYPALSPVDAQIVEAARSAAREIYGHDPIMQPLVA
                     GSGPMHPLSTALGIPAVLAGIGYADTRAHAPDENIRMSDYWEGMKFIGRLIDRFARTA
                     "
     gene            2045..>2674
                     /locus_tag="VFF70_15310"
     CDS             2045..>2674
                     /locus_tag="VFF70_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZY46106.1"
                     /translation="MILSALQGGPGELLPAVIAYCIIVLVCFPIHEFGHAWVATRLGD
                     YVAQQEGRVTLNPAAHIDPFGAIILAVAGFGWAKPVPFIPGNLRKAPSISVGIILVSI
                     AGIVMNLFLAALAALPFRLHLVSVADISNTQSVAFILYLIISINAVLAIFNLIPIPPL
                     DGSKILESLLPPQQRGIMDFLNQYGFYILILLFILPGSLLFQLIGPLQLG"
BASE COUNT          544 a          754 c          679 g          697 t
ORIGIN      
        1 tctaaatagt cggttgtcat tgtaatttcc cctgcgcaga aaacactact tcgttacaat
       61 agcctgaatc cgctcggcga tttccggttt ttcgaaaacg agcacattgc gttgaacgaa
      121 tttggctaac agcgccgtgc cattttcagt gaggcggtag tatcggcgct gcgggccaac
      181 atcggactgc ttcaattcgc tttggacgat gcctaacggc tcaaaacgat tcagcgcgcg
      241 gtaaatgctg ttttcgtcgg cggtaatcgt gccttcgctc agctcgccaa tcgcggcgct
      301 catttcgtaa gcatagcccg gacgatcgtg cagcagcaaa agtatccaaa aagtgagcag
      361 ccctttctta tagacctctt cccagttctt tagtagctcg tctatctctg ctgtgttttt
      421 gcgcggcatt cggctttcga ctccactatt attcctatgg tataatagca tgcctcagtt
      481 ggtttgtcaa tatctaaggt ggactgcagg cgaaatgacg aggagaccct tcgaatttag
      541 gctatgctgt tcgtgcaaat cgatctatca atcggccaat aaacttcatg ccttcccagt
      601 aatcactcat tcgaatgttt tcgtcgggtg cgtgcgcgcg tgtgtcggcg taaccaatcc
      661 cagccaagac cgccggaatg ccgagcgcgg tcgagagcgg atgcatcggc ccgctgcctg
      721 cgaccagtgg ctgcatgatc ggatcatggc cgtaaatttc acgcgctgcc gatcgtgccg
      781 cttcaacgat ctgcgcatcg acgggcgaca gcgcaggata ttcggcgctc atcggcacga
      841 tctccacatc cgtgaagccg cgtcgatcga gatgcgcgcg cagcagagac aggcaaagtt
      901 ccggcgtgag attaggcacc aaacggaaat ccattttggc aaaggcgtaa ttgggcaaca
      961 cggtcttcat gccttcccca gtatatccgg cgtgaatgcc gcagatcgtc gcggttgggc
     1021 catatagaaa tctcttcaat aaatccaggc cggtttcgtt ttcgatgaat cgatcgaagc
     1081 cgaacatctt tttcagcgcc gtttcatcga acggcatcgc gcgcaacatt ttcagttcgg
     1141 cttcgttggg ctggcgcaca tgtgcgtaat acccgtcgat caaaatgtta tcgtgctcgt
     1201 ccttgatcgt attcagcgcc catatcaagc gccagaccgg gctgggcaca atcgccgcat
     1261 agccggaatg cagatcgcgt ctcgcgccgt gcacgcgcag ctccaaatat tgaataccct
     1321 ttaagcccat gcttagcaca tatcgatcgg cttcgtcttt gccgccgcct tcccacaaac
     1381 acccgtcggc cttcaatatg tctttgtgct tttcggcgaa ctcggccaga ttttcgctgc
     1441 ccacttcttc ttcaccctca atgacccact tgatctttaa gggcagcgcg ccgatcgttt
     1501 tttgccatga cgcgatcgcc tgaatgcggc agaccagatc gcccttatcg tcactgaccc
     1561 cgcgcgcgaa gagcttgcca tcgcgcagtg tcggctcaaa tggcggcgtc gtccacagat
     1621 cgatcggatc ggcgggctgc acatcgtagt gattgtagat catgagcgtg cgcggcccgc
     1681 tgcccagttc ggcatacacg atcggcgcgc ggcccgtcgg gaactgctgc accttcgcgc
     1741 cgatcgattc caggcgcccc tgtaggagat tcgccatctc gtgcacgccc tcgcccgtcg
     1801 cggcgatgct gggctggcgc agaaacgctt tgagatcgtc aatgaacttt tcttggtggc
     1861 cagcgatata gttatcaaac gagttgaaat ccataagact ccctgaataa atagtgtggt
     1921 ctgagtgtcg aaggctgccg gttacgtcat gcggtgtaaa gtttaacacg taacacccaa
     1981 cacgcaacac tcgcgccgtg ttataatttg cgagcgcagt tgattttttc gaaacgaggc
     2041 tcgcatgata ttatcggcat tacaaggcgg cccaggtgaa ttactccccg ccgtgatcgc
     2101 ctactgcatc attgttttgg tgtgctttcc cattcatgag ttcggccacg cgtgggtcgc
     2161 cacgcgcctg ggcgattacg tcgcccaaca agaagggcgt gtaaccttga atccagcggc
     2221 gcatatcgat ccgttcggtg caattatcct ggccgtggcg ggctttggct gggccaagcc
     2281 ggtgcccttt attccgggca atttgcgcaa agcacccagt atttctgttg gcattattct
     2341 ggtttcaatt gccggcatcg tcatgaattt gtttttggct gcgctggcgg cgttgccctt
     2401 ccggttgcat cttgtttcag tcgccgatat ttccaacacc caatcggtcg ccttcattct
     2461 gtatctcatc atttcgatta atgccgtgct ggctattttc aatttgattc ccatcccgcc
     2521 gcttgatggg tcgaaaatat tggagagcct tctaccgccc cagcagcgcg gcatcatgga
     2581 ttttcttaat caatacggtt tctacatttt gattttattg ttcattctgc cgggcagtct
     2641 tctatttcaa ctgattggtc cgctccagct gggc
//
LOCUS       DASRTJ010000758         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_222323,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000758 DASRTJ010000000
VERSION     DASRTJ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222323"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFF70_15315"
     CDS             complement(<1..499)
                     /locus_tag="VFF70_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis protein"
                     /protein_id="HZY46107.1"
                     /translation="MKTRLRNIGLALKVLPRILIDPRQRNLFREMRVFIRQLPIVLKA
                     PIPEALQSITPTTSSRSSSAQATRELADVVALLDRDSPLGLCLRRSLTRYHFLRELGV
                     PVTVHFGAKLIGGKADRPALRHAVGQVSITGHAWLTLDDQIYFEASENYRGFSVMLTF
                     PQTELE"
     gene            complement(496..771)
                     /locus_tag="VFF70_15320"
     CDS             complement(496..771)
                     /locus_tag="VFF70_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZY46108.1"
                     /translation="MDATAKLKRSDRVTHQVVAEEAILIHLDTGTYFSLNKIGTEFWD
                     MLDGEHSIQQHAETIAQKYQVETPLVVADLLELAGKMAADQLVEAVR"
     gene            903..1883
                     /locus_tag="VFF70_15325"
     CDS             903..1883
                     /locus_tag="VFF70_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46109.1"
                     /translation="MHWDLHGQTISGSTNNDLLRERWRASFASRSISNRRIDLSFALD
                     LVADIPARPIGEPHYRQADLLEYTIDGQNVVAHFPRFGQLRLDLVHGLTEGHIVDAAL
                     NTYGVLEDLIAIGLSPHLRRRGMFLIHAFGAATADPHPRPRPLSLKEERGVLLVGNIG
                     AGKTTTGMSLLDAGWKLLSNDSPIVKATGEVLSYPSLLAAYPDTFDRFERSRKFAQTT
                     LNTEGRQKITLSAELIWPDVWIDRAMCCAIFFPQIESRSDHAIDPIDPPEALRRLLPH
                     AIEQWDREMIAEHLKVLKNLVENAPAYLLRLGSQVKTIPAQVISTLNQLQ"
     gene            complement(1890..>2198)
                     /locus_tag="VFF70_15330"
     CDS             complement(1890..>2198)
                     /locus_tag="VFF70_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46110.1"
                     /translation="IKYVRIYYLDTAATDMTVWLTRYEPGQDNLDLVSVKSTGNTGYG
                     SVLSSLITQTVDMANYAYVLTWGTSVATSANQICGIRVAYYAPPFSALFLPIIRKNSP
                     "
BASE COUNT          495 a          606 c          593 g          504 t
ORIGIN      
        1 cctccaattc ggtttgcgga aaagtgagca tcacactaaa gccgcgatag ttttcactag
       61 cttcaaaata aatttgatca tcgagagtca accaggcgtg accggtgatc gacacttgcc
      121 ccactgcgtg gcgcagtgcg ggtcgatcgg ctttgccgcc gattaatttt gcgccgaagt
      181 gaaccgtaac cggcacaccc aactcgcgca ggaagtgata gcgcgtcagc gatcgacgca
      241 aacacaggcc gagcggcgaa tctcgatcga gcaaggcgac gacatcggcc agctcgcgtg
      301 tagcctgtgc agaactcgat cttgatgagg tcgtcggcgt gatcgattgc agggcctccg
      361 ggatgggcgc tttcaaaacg atcggtagtt gccgaatgaa gacgcgcatc tctcgaaata
      421 aattgcgctg gcgcggatcg atcaggatgc gcggcagaac cttcaacgct aaaccgatat
      481 tccgtaagcg agttttcatc ggacggcttc gacgagttga tcggccgcca tcttcccggc
      541 cagttccaat agatcggcga cgaccagcgg cgtttcaact tggtacttct gtgcgatcgt
      601 ttcggcatgt tgttggatcg aatgttcacc atcgagcatg tcccagaact ccgtgccgat
      661 cttgttcagc gaaaaatatg tgccggtatc caaatggatc aagatcgctt cttcggccac
      721 gacttgatgc gtgacccgat ccgagcgttt gagctttgcc gttgcgtcca caatcgtttc
      781 cattgattcg agtataatca tttcagaatt agcgaaggcg actccgtgcg gagcctccct
      841 gtgggacacg ccgccggatt cggacatacg cgcccgaacc acttgtttct gagagagatt
      901 ctatgcactg ggacttacac ggtcaaacca tcagcggctc aaccaataac gatctcttgc
      961 gcgagcgttg gcgcgcttcg tttgcgtcgc gatcgatcag caatcgccgg atcgatcttt
     1021 ctttcgcgct tgatctggta gccgatattc ccgctcgccc gatcggtgag ccgcactatc
     1081 gacaggccga tctgcttgaa tacactatcg atggacaaaa cgtcgtcgcg cattttccgc
     1141 gcttcggtca actgcggctc gatctggttc acggcctgac tgaaggacat attgttgatg
     1201 ccgcgctcaa cacctatggt gtccttgaag atttgatcgc gataggtttg tcgccgcatc
     1261 tgcggcggcg cggaatgttt ttgattcatg cgtttggggc cgcaaccgcc gaccctcacc
     1321 cccggcctcg gcccctctcc ctaaaagagg agaggggagt gttgctggtt ggcaatatcg
     1381 gcgcgggcaa gacgacgacg ggcatgtcgc tgctcgatgc cggttggaaa ttactatcga
     1441 acgattcgcc gatcgtcaag gcaacaggcg aagtgctgag ttaccccagc ctgcttgccg
     1501 catatcccga tacgttcgat cgattcgaga ggtcgcgcaa gttcgctcag acaacgttaa
     1561 acactgaagg ccggcaaaaa attacactgt ccgccgaatt gatctggccg gacgtgtgga
     1621 tcgatcgagc gatgtgctgc gcaatcttct ttccgcagat cgaaagccga tcggatcacg
     1681 cgatcgatcc gatcgatcca ccggaagcgc tgcggcgctt gctgccccac gcgatcgagc
     1741 aatgggatcg cgagatgatc gcggagcatc tcaaggtgtt aaagaacttg gttgaaaatg
     1801 ctccagctta tcttttacgc ttgggatcac aagtcaagac tattccggcc caggtgatct
     1861 caacgctcaa tcagttgcag tagtccacac tagggactgt tcttccgaat gatcggcaga
     1921 aacaaggctg aaaagggtgg agcataataa gccacccgta tcccgcaaat ctggttcgct
     1981 gatgtcgcca cgctcgtgcc ccaggtgaga acgtaggcgt aattagccat atcaaccgtt
     2041 tgcgtgatga gtgaactcaa aacactgccg taaccggtat tgcctgtaga tttgacgctg
     2101 accaaatcca gattgtcctg acccggctca tagcgcgtca accacacggt catatctgtc
     2161 gctgcggtat ccaagtagta gatccgcacg tatttgat
//
LOCUS       DASRTJ010000759         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_180972,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000759 DASRTJ010000000
VERSION     DASRTJ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180972"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(714..1985)
                     /locus_tag="VFF70_15335"
     CDS             complement(714..1985)
                     /locus_tag="VFF70_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZY46111.1"
                     /translation="MPPKSVDEQASYAAQSVDLHRRYQGALEIKSKVPIKDEHILSLI
                     YLPPGAVEPARTIAQHPEWVYDYTIKRNLVAIVSDGSAVLGLGNIGGRAAMPVMEGKA
                     VIFHTFGGVEAFPICLTTQDQDEIVEIVKRLEPTFGGVNLEDISAPRCFYIEDRLRKE
                     TNIPIFHDDQHGTAVVVLAGLMNACKITGRKLKDISIVVNGAGASAIAVSKLLISVGV
                     GDLILCDTTGTIYKGRPKGMNWIKNEMAEITNAENVRGTLVDAMRGRDLLIGLSVAGA
                     VKPEMVQAMNKNPIIFALSNPTPEIFPDEAKKVGALICATGRSDLPNQVNNSLAFPGI
                     FRGALDTRAKQITDQMKIAAAHAIASIVTDKELNPELIIPKGMDFRVAPKVAAAVAKV
                     AMETGQARQMVDVATVEERCRAFVYEGQQLA"
     gene            complement(2130..3047)
                     /locus_tag="VFF70_15340"
                     /pseudo
     CDS             complement(2130..3047)
                     /locus_tag="VFF70_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
BASE COUNT          646 a          859 c          837 g          755 t
ORIGIN      
        1 gattatttgg tagctgtgca gtatagcccg gctgattcgg cgcagtcgtt gccgcaggcg
       61 cggcggctgg cgcacaggcg gaaacgatga tactgatcgc cagcaaggcg attaaggtgg
      121 cacgcgatcg tgaccaagca aacgaatgag gcgacatttt ctcttctcct tgtcgagtca
      181 gtgtgcctgc gcagatttcg tgcgtcagcc ttgaacggca cgcagcctgt gcaaggttat
      241 ggatgtcagt cgatcagcgt atcgaccgag aagcgacggg gccccatcac tgtctgtttt
      301 ctagacgttg aagtgtgcag aatgttcctg atgtaatctg agattgagct tagcaatctg
      361 aatttaattg aagtaccctg agcggtgcga gtgcccatcg acttctgtat cactcgcaaa
      421 gtgagtgata ccccgctcag gccattcccc agccataggc tgcgggcgtg ctaggcggga
      481 tgcttgcgcg ctgcgcagtc aacgcctagc gacctacctg agcactttgt actcagggaa
      541 agggcggcaa tcttcaggat tgtcaatgag attgcttctg cttcgcttag agtcgcaatg
      601 acggctcaat ttttcatgct ggcgctaagt aacttttcct aaactgtgtt agcaggacaa
      661 taaaaatcgg gcgatagttc aactattgcc cgatctattg agaggagtga gacttacgct
      721 aactgctggc cctcatatac aaatgctcga cagcgttcct cgactgttgc tacatcaacc
      781 atctgccgcg cctgtccggt ttccattgct accttcgcca cggcggcggc aacttttggt
      841 gcaacgcgga agtccatgcc tttgggaatg atcaattcgg gattcagttc tttgtcggtg
      901 acgatcgatg caatcgcatg cgccgccgcg atcttcatct gatcggtgat ctgcttggct
      961 cgagtgtcca acgcgccccg gaagatgcct ggaaatgcga gcgagttgtt gacttgattc
     1021 ggtagatcgc tgcggccagt ggcgcagatc aatgcgccca ctttcttcgc ttcgtccggg
     1081 aaaatttctg gcgtgggatt ggatagcgca aagataatcg ggtttttatt catagcttgc
     1141 accatctctg gttttaccgc gcccgccacg gataagccaa tcagtaaatc ccggccgcgc
     1201 atcgcatcga ctaatgtgcc gcgcacgttt tcggcgttgg tgatctcggc catctcgttc
     1261 ttgatccagt tcatgccttt ggggcggcct ttgtagatcg tgccggtggt gtcgcacaat
     1321 attagatcgc ccacgccgac gctgatcagc aacttgctaa ctgcgatcgc actcgcgcca
     1381 gcgccattga cgacgatcga tatatctttc aattttcgtc cggtgatttt gcacgcgttc
     1441 atcagcccgg ctagcacgac gacggcggtg ccatgttgat cgtcgtggaa gatcggaatg
     1501 ttggtttctt tgcgcaatcg atcttcgatg tagaagcagc gcggtgccga aatatcttcg
     1561 aggttcacac cgccgaaagt cggctcaagc cgctttacga tttcaacgat ctcatcttga
     1621 tcttgcgtcg ttaaacagat cgggaatgct tcgacgccgc cgaaggtgtg gaatatcact
     1681 gccttgcctt ccatcaccgg catcgccgcg cgcccgccga tgttgcctaa acccaacact
     1741 gccgatccat ccgacacgat cgcgaccaag ttgcgtttga tcgtgtaatc gtaaacccat
     1801 tcgggatgct gcgcgatcgt tctggcgggt tcaaccgcgc ccggcggcaa atagattaaa
     1861 ctcaaaatgt gctcgtcttt gatcggcact ttgcttttga tttcaagcgc gccctgatag
     1921 cgccgatgca gatcgacgga ttgtgcagca tacgatgctt gctcatcgac cgattttggc
     1981 gggatgggaa agtgtccttc gtaaatatat ctgcgggttc gctcggcaat ccgatcggga
     2041 tctgcggcca cttttgctat gccggtatcg atcgccgctt tggcgacggc tcgcgcgact
     2101 gcgggcgcga cacgaaaatc gagcactttc ggaatgatgt aattcggatt taattcttta
     2161 tcggtcacaa tcccggccaa ggcttcggcg gcagccaata acatttgatc attgacctcg
     2221 cgtgcgcgca cgtcgagcag gccgcggaac aagccgggga aaaccgaggc gatgtccatt
     2281 tgatttggat gatgcgactg ggccgtggcg atgatcgccg cgcccgccgc ctgcgccgct
     2341 tcggggtcga tctcaggcgc cggtccggcg aacgcaaaca cgatcggttt tgtcgccatg
     2401 ctcttgacca tatcttgcgt cacgacatcg ccgaccgaaa aaccgatgaa tacatccgcg
     2461 cctttgatca cgctcgccag atcgccacgc actcgatcag ggttcgtctt cttcgcgatc
     2521 tcccacttgg cccagtgcat cgattccggt cgatacggat acagcgcgcc atgacgatcg
     2581 catacgatga tgttcttcac gccgatcttc aataaaattt ctgcagtgcc gatgcccgcc
     2641 gcgccggtgc cattgatcac gactttcaga ttctcggctt ctttgccgac aattgtcaat
     2701 gcattgagca agccggccag tacaccgacc gccactgatt cgcggtgatt gttgactacc
     2761 ggaatgtgca tcgcgcgttg aagttcttcg ccgatcgtga ggcaggccgg cgcgacaata
     2821 tcttccaaac aaataccacc gaacgtcggc gcgagtgcga tgactgtttc gacgatctcg
     2881 ctgatgttgt gcgtgctcaa gcagatcggc atcgcatcaa cacccgcgaa ggttttgaaa
     2941 atcacgctct taccttccat tacgggcagg atcgcttccg gtccgacaga gccaagcccg
     3001 aatgccgacg aaccatcgga gacaagcgca acggtgttgc cacgcatggt cagcgcaaag
     3061 gattcgactg ggtctttttc aatatcgagg caaggtt
//
LOCUS       DASRTJ010000760         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_222997,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000760 DASRTJ010000000
VERSION     DASRTJ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222997"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /gene="murJ"
                     /locus_tag="VFF70_15345"
     CDS             complement(<1..1304)
                     /gene="murJ"
                     /locus_tag="VFF70_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZY46112.1"
                     /translation="MTPTNSSHRQIARAAAVVMITFVASRLLGLLRDVVISAHFGTSD
                     ALDAYYAASRLNDLIFNLIAGGALASAFIPTFAGYLARDDDEGAWRLASNIINIMLLA
                     LAVVSLLAAIFAFPIVSGLLGIGFDAEKQKLTADLLRFLLIAPTIFGISGVIMGILNA
                     KQNFWLPGLAPSMYNLGMIGGVIFLSSSFGIYGLAIGAVVGAALHLIVQIPGLIKAGA
                     RLTPRLGLHDPGVRQVFRLMAPRVLGLATVQLNFIVETILASTLGTGAVSALNYAWRV
                     MLLPQGVVAQSVAIAAFPTFSDQYARGKIDELRSALSATLRVILFIAIPAAIGLLVLR
                     VPLIQLLFERGQFQDTSTELVATALGFYALGLIGHSGVEILTRAFYGMHDTRTPVLLS
                     VLSLALNLILSLALIGPMQIAGLAFANTVAILIEMILLIFVLR"
     gene            1715..2086
                     /locus_tag="VFF70_15350"
     CDS             1715..2086
                     /locus_tag="VFF70_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZY46113.1"
                     /translation="MTALEIDPGVEQRLQTERNIWLATVRADGRPHLAAIWFVWYAQR
                     VYLCTAATSIKARNISTNPNVTLSLEVGDKPIVLEGSAKAIERAEPTIVAAFQSKYNW
                     DITTDREYDRVIEIEVERIRA"
     gene            2111..>3090
                     /locus_tag="VFF70_15355"
     CDS             2111..>3090
                     /locus_tag="VFF70_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZY46114.1"
                     /translation="MRFDRFTERAQDAANRSIEILQRYGHNQVDTEHLLLALLEQPDG
                     LVSQILEKLNADVTVMRGKLEELLKQSPKAAIYGGGTGQVFITPRVKRVLDLAQEEAN
                     RLKDEYISTEHLFLAIGTERNTAAARLLSEAGVTRERINETVKSIRGGQRVTDPGAES
                     RYRTLEKYSRDLSALAKAGKLDPVIGRDDEILRVIQVLSRRTKNNPVLIGEAGVGKTA
                     IVEGLAQKIANNDVPEILMGKRVVALDLGAMVAGSRFRGEFEERLKASMEEIQRAQGE
                     IILFIDELHTVVGAGAASGALDAGSMLKPALARGELQCVGATTLDEYRKY"
BASE COUNT          789 a          822 c          892 g          587 t
ORIGIN      
        1 cgcaaaacaa atatcagcag gatcatttca atcagtatcg cgacggtgtt ggcgaatgcc
       61 agtccagcga tctgcatcgg accgatcaac gcgaggctga gtatcaaatt gagggccagc
      121 gataaaacgc tcagcagcac cggagtgcgc gtatcgtgca tgccataaaa cgcgcgcgtc
      181 aatatttcca cacccgaatg gccgatcagg cccaacgcat aaaagcccaa cgccgtggcg
      241 accaactcgg ttgatgtatc ttggaactgt ccgcgctcga ataacagttg aatcaatgga
      301 actctgagga cgagtaaacc gatcgctgcc ggaatagcaa tgaacaagat cacgcgcaag
      361 gtggccgata aagccgatcg aagttcgtcg atcttgccgc gcgcgtattg atcggaaaaa
      421 gtagggaagg ctgcaatcgc caccgattga gccaccacgc cctgcggcag caacatcact
      481 cgccaggcat aattgagcgc cgagactgcg cctgtgccca acgtcgaagc gaggatcgtt
      541 tcgacgatga agttcagttg cacggttgcc agccccaaca cacgtggtgc catcaagcga
      601 aagacttgcc gcacgccggg atcgtgcaga cccaatcgag gtgtcaagcg cgcgcccgct
      661 ttgatcaagc cgggaatctg cacgatcaaa tgcagcgccg cgccgaccac tgcgccgatt
      721 gccaatccat aaattccaaa cgatgacgaa agaaagatta cgccgccgat catgccgagg
      781 ttgtacatgc tgggcgcgag gcccggcagc cagaaatttt gcttggcatt gagaatgccc
      841 atgatcacac cgctaatgcc aaagatcgtt ggagcgatca acaaaaagcg caggagatca
      901 gccgtgagtt tttgtttttc ggcgtcaaag ccaattccga gcagcccgct gacgatcggg
      961 aaagcgaaga tcgcggcgag gagcgaaaca acagccaagg cgagcagcat gatgttgatg
     1021 atgttgctgg ccaaacgcca agcgccttca tcgtcatcgc gggcaaggta accggcgaac
     1081 gtcgggatga aagccgaagc aagagcgcca cccgcgatca gattgaagat caaatcgttg
     1141 aggcgcgatg cggcataata cgcatcgagc gcgtcggaag tgccaaaatg tgcgctgatg
     1201 accacatcgc gcagcaggcc gagcaatcgg ctggcgacga aagtgatcat caccaccgcg
     1261 gcggcacgcg caatttgtcg atgcgaggaa tttgtcggtg tcacgagcgg cgattatacc
     1321 actctgatga gggacatgtg aaaagcgatt gcgcgcaaat attcctccct ctgaggtcat
     1381 tttccatttc ggggaggggt agacgccaaa gagccagtgg tcgctgtcca ctggctcttt
     1441 agcaaaaaca aggaggagaa gaagagatgt ttcgcccgct actcttcgat gataaattta
     1501 ccgcatatcg ctctacttaa ctcgactaaa tgcctacgat tcccctacga aacgcgctaa
     1561 aataggctag atcgatgctg gttggtggtt cagcggccac ccgattgttc tcccacgctc
     1621 gacagccgct ctaactcact cttagcgact tttctaacac tcctctagta caaaacggct
     1681 aaactccagc caatatattg tataatcagg gcagatgacg gcactcgaga tcgatccggg
     1741 cgttgagcag cgattgcaga ctgagcgcaa tatctggctg gccaccgttc gagcggatgg
     1801 ccgtccgcat ttggcggcga tctggttcgt atggtacgca caaagagttt atctctgcac
     1861 ggccgccacg agcattaaag cgcgaaatat cagtaccaat ccaaatgtca cgctgtcgct
     1921 ggaagtcggt gacaaaccga ttgtgctcga gggatcagct aaagcgatcg agcgagccga
     1981 accaaccatc gtcgctgctt ttcaatcgaa atataattgg gacatcacaa cggatcgaga
     2041 gtacgatcga gtgatcgaaa ttgaagtgga acgaataaga gcctagggcg gacaaggaaa
     2101 aataactatg atgcgattcg atcgattcac cgagcgcgcg caggatgcgg ccaaccgcag
     2161 cattgaaatc ttgcagcgct acgggcacaa tcaagtggat accgagcatc tgctgttggc
     2221 gctgctggag caacccgacg gcttggtctc gcaaattttg gagaaattga atgccgatgt
     2281 gacggtcatg cgcggcaagt tggaagagct tttgaagcaa tcgcctaagg ccgcgatcta
     2341 tggcggcggc acgggccagg tcttcatcac accgcgcgtg aagcgtgtgc tcgatctcgc
     2401 ccaggaagaa gccaatcggt tgaaggacga atatatttcg accgagcatc tctttttggc
     2461 gattggcacc gagcgcaaca cggcggccgc gcgcttgtta agcgaagcgg gcgtgacgcg
     2521 cgagcgcatc aacgaaaccg tcaagtcgat tcgcggcgga cagcgcgtga ctgatccggg
     2581 cgcggagagc cgctatcgta cgttggaaaa atacagccgc gatctgagcg cgctcgcgaa
     2641 agcgggcaaa ctcgatccgg tcattggccg cgatgatgaa atcctgcgcg tgattcaagt
     2701 tttatcgcgc cgcaccaaaa acaatccggt gttgatcggt gaagcgggcg tgggcaagac
     2761 tgccatcgtc gaagggctgg cgcagaagat cgccaacaat gacgtgccgg aaattttgat
     2821 gggcaagcgc gtcgtcgcgc tcgatctggg cgcgatggtc gccggttccc gtttccgcgg
     2881 cgagttcgaa gagcgcttga aagcatcgat ggaagagata cagcgcgcgc agggcgagat
     2941 cattttattc atcgacgaac tgcacaccgt cgtcggcgca ggcgcggcgt caggcgcgct
     3001 ggatgcgggc agcatgttga agccggcgct cgcacgcggt gaactgcagt gcgtcggtgc
     3061 gacaacgctg gatgaatatc gcaagtatat
//
LOCUS       DASRTJ010000761         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_181189,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000761 DASRTJ010000000
VERSION     DASRTJ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181189"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VFF70_15360"
     CDS             <1..1527
                     /locus_tag="VFF70_15360"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZY46115.1"
                     /translation="AVMGDDGEKFGMWPTTFEHCWAGGWMDALFTALEQNSDWLITCP
                     PAEVLDLIAPLGRVYLPVASYDEMGEWSLPAPLAREFAQVKERLQIEGREDVLRFLRG
                     GMWRSFMVKYPEINTLHKRMLWASDRVHRMKKAAARSEALDHLWASQCNCPYWHGVFG
                     GVYLFHIREANYAHLIEAQNIADRASHPAETWIEVEQVDVDRDGHAEAVIASDAQWLM
                     FSPGVGGALLEWDWRKRGVNVLNTLARWREGYHQDLIDAAAKGEVVIAGQSHKLESIH
                     TSVVKAKEPGLEKRLIVDWYRRSSLIDHIIAPDASSDAFYRASYAEWGDFVNQPYAVK
                     INRSAKKITLTLVRAGGVWIDTVRHPLRIEKKITVESGQSAVAVAYTLTNVGAEKISA
                     RFGVETNWGISGGDATEGAYSLWPGGNLIRLNAIDAVPSSKEVAIVNERVGRGLIQLS
                     EAAAWWQFPIETISLSEAGFEKTYQGTTLLAHWPLDLEPGAMWKMKAQIELMHGGNAG
                     "
BASE COUNT          406 a          446 c          569 g          403 t
ORIGIN      
        1 gcggtgatgg gtgatgacgg cgagaaattt ggaatgtggc cgacgacctt cgagcattgt
       61 tgggcaggcg gctggatgga tgcgctcttc accgcgctgg agcagaattc cgattggttg
      121 atcacttgcc cgccggccga agtcttggat ctgattgcgc cgttaggccg tgtgtatttg
      181 cctgtggctt cgtatgatga aatgggcgaa tggtcactgc ccgcgccgtt ggcacgcgag
      241 tttgctcaag tgaaagagcg tctgcaaatc gaaggccgtg aagatgtgct gcgtttcctg
      301 cgcggcggca tgtggcgatc gttcatggtg aaatatcccg aaatcaacac actgcacaag
      361 cggatgctgt gggccagcga tcgtgtgcat cgcatgaaga aagccgccgc tcgatcggaa
      421 gcgcttgatc atttgtgggc gtcgcaatgc aactgtccat actggcacgg cgtcttcggc
      481 ggagtttatt tgttccatat ccgcgaagcg aactacgcgc atctgatcga ggcgcagaac
      541 atcgctgatc gcgcgtcaca tccggctgaa acttggatcg aggtggagca ggttgatgtc
      601 gatcgcgacg gacatgctga agcggtgatc gccagcgatg cccagtggct catgttctcg
      661 ccgggcgtcg gcggtgcgct gctcgaatgg gattggcgca agcgcggcgt gaatgtgctc
      721 aacacgctgg cgcgttggcg cgaaggttat caccaggatt tgatcgatgc ggcggcgaaa
      781 ggtgaagtcg tcatcgccgg ccaatcgcac aaactggaga gcattcacac ctcggtggtt
      841 aaagccaaag agccgggact tgaaaagaga ttgatcgtcg attggtatcg ccgatcgagt
      901 ttaatcgatc acattatcgc gccggatgcg tcgagcgatg cgttctatcg ggcgagctat
      961 gccgagtggg gcgatttcgt caatcaaccg tatgcggtga agatcaatcg atcggccaag
     1021 aagatcacgc tgacgttggt gcgggctggc ggcgtgtgga tcgatacggt gcgccatccg
     1081 ctgcgcatcg aaaagaaaat taccgttgaa tctggccaat ctgcggtggc cgtcgcttac
     1141 acgctgacga atgtcggcgc agaaaaaata tcggcgcgtt tcggcgtcga aacgaattgg
     1201 ggcatctccg gcggcgatgc aaccgagggc gcgtactcgc tgtggccagg cggcaatctg
     1261 atccgcttga atgcgatcga tgcggtgcct tcctcaaagg aagtggcgat tgtgaacgag
     1321 cgcgtcggtc gcggcttgat tcaattgagt gaagcggcag cgtggtggca gttcccgatc
     1381 gaaacgattt cgctgtcgga agcgggcttc gagaaaacgt atcaaggcac aacactgttg
     1441 gcgcactggc cgctcgatct tgagccgggc gcaatgtgga agatgaaggc gcagatcgag
     1501 ttgatgcatg ggggtaatgc aggatgatcc actaatttgc acgaatcgac gcgaatattt
     1561 cttccgcaag gtaacaccaa gactttttca actggcattt cgaacgagcg aaacgagtga
     1621 gaaatctctg gtactgatga agatcgttta ctacttaagg cagagattcc tcgctgcgct
     1681 cggaatgact cgagaataaa cgaaaacggc tcgatcgaaa ttcgatcgag ccgctgtcta
     1741 tttgaatcag aaaatatttt tacgctttga tccccaacac ccgctccacg ctttgcagca
     1801 gttcctgcgg accgaacggc ttgg
//
LOCUS       DASRTJ010000762         9630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_223486,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000762 DASRTJ010000000
VERSION     DASRTJ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223486"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VFF70_15365"
     CDS             <1..1492
                     /locus_tag="VFF70_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZY46116.1"
                     /translation="TSADVVTSFLYDAATDQFFHGSALGGTGEWTDTKAIRRDGMTYR
                     VIQQGQPILVGDTLQEPETNPGLIQSGVRSLICVPLISKGKPLGVLFVNSFSPYKFDA
                     DDVQIASALANQAASAIENARLFADIAEGRDKLQAILNSTHDGILMFDASTRIVTVNP
                     RLEEIWDLSRVSLEDRLLIDLLGQPEFQIAAKLGFTADKLHHQLLQVQAGERIEWPKE
                     IYSLPGSRPRFIERSGLPVLDIANRLIGWMLVLHDVTEERELQQSRDDLSNMIVHDLR
                     SPLTSILGSLQLLEENIQEKDAASIEQQALAISTRSTQKLLDLVNSLLDINKLTAGQV
                     TIESAPDSLALSINAAIERLSVLAVEEGVIIRKQIPPELPPVLIDEEKITRVLINLID
                     NALKFSPHGGQIMVLAEVWPHDGASPAFVRCAVLDSGPGVPLEFRERIFDRFVQVTHQ
                     PGRRHGTGLGLSFCRLTVEAHGGKIWVDDAPGKGSVFSFTLPIAKV"
     gene            1727..3625
                     /gene="nusA"
                     /locus_tag="VFF70_15370"
     CDS             1727..3625
                     /gene="nusA"
                     /locus_tag="VFF70_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZY46117.1"
                     /translation="MKSEFLLAFNQICTERNLPKEVVLEALSTALVSAYRRNINASSA
                     QNVTAVIDGTTGVAQIYAEKEVAEDVTDVRTEVTVAAARQIMPEAKVGDMVMVESTPD
                     GFGRIAAQTAKQVILQRIREAERDAQYNMYAEREEEIINGVVQSIGPSGITLNLGRTE
                     ALMPKKEQVPGERYQMHQRLRAYVLEVRKSTRGPQIIVSRSHKNMLRRLLELEVPEIF
                     AGTVEIKSIAREPGARSKIAVAATQAGIDPVGACVGMRGVRIQSIVNELGGEKIDVIE
                     WNLDPAAYIQKALSPARVLTVHPDDDPREGKIALVVVPDDQLSLAIGREGQNARLAAK
                     LTGWRIDIQGSTESLRKALAELEADPEISATMGADATAIPSTQAALQRHTEQQLPWNQ
                     EEMQSIKHVLDGVHRVLTARKQAERERLRAQIQAERAIKAQEEAVEVSRKEQLAAYKA
                     RIPAQAYETMLADLGISARVLNHLEKSDLTNVGQIHERLVSSDESMLSIEGIGPKGLT
                     EIKQAIEEKGLGLMPLPEPVVMETSTEAGAGQPIEPAQPIGAEAAAGAATAEAVSPIK
                     PIEGAPAIELEEVDDDDDPDKKLKKKGKRKDRTLVLDEKSGVVVAKKQRKTGRATDIF
                     ELIDEEEI"
     gene            3694..3984
                     /locus_tag="VFF70_15375"
     CDS             3694..3984
                     /locus_tag="VFF70_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZY46118.1"
                     /translation="MAGKHIPQRTCVGCREVQGKRQMVRVVRRADGQVSVDATGKAPG
                     RGAYIHQRQSCWQYALKSGHLTHALRVTQLAADDRLALQAYAAALPKDETES"
     gene            4316..6103
                     /gene="infB"
                     /locus_tag="VFF70_15380"
     CDS             4316..6103
                     /gene="infB"
                     /locus_tag="VFF70_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZY46119.1"
                     /translation="MAEAVKTSTVVEIPSALTVRDLAERVKVSPINIIRELMNNGVMA
                     SINQQIDYDTAAIVLQGMGFETHEEERAEESPSEDLSMTLWQRLYAKEDPAKLEARPP
                     VVTVMGHVDHGKTSLLDVIRNTSIAAGEAGGITQHIGAYQVERNGRKITFLDTPGHEA
                     FTAMRARGAQATDIAVLVVAADDGVMPQTKEAIAHIKAAHVPIIVALNKIDKSNANPE
                     RVKKELNDNGVVIDEYGGDILLVPVSAKQKKGIEDLLEAILLVADSREIKANPDRFAV
                     GTVIEGRLDKSLGAVATVLVQNGTLKAGDGIVLGKVAGRVRTMYDDKGKAIQKAGPST
                     PALISGLIGVPSAGDQFEAVKDEKIAKAIIGAKTKAEASDGVVRKTLTLADVFSRIQA
                     GEVKELPIILKADVQGSLEPVINQLEKLNTTAVKVKLLHSSTGTIGENDVQLAIASGA
                     IVVGFNVTADGSAKRLADAEHVDVRTYDIIYRLTDDIQKALTGMLEPVYKEVSLGVAE
                     VRQVFKIKGVGNIAGSMVREGVIQRGVKARVKRGGEVVHEGGVGTLKRNTEDVKEVKA
                     GFECGIGLANFQEFKKGDLIEFYTQEQVS"
     gene            6184..6531
                     /gene="rbfA"
                     /locus_tag="VFF70_15385"
     CDS             6184..6531
                     /gene="rbfA"
                     /locus_tag="VFF70_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZY46120.1"
                     /translation="MTTRRQERVAEQIKIELGKMLEHQINDPRLNLISITDVTISSDL
                     REANVYTSALAGQSVEAEVLAGLEHARSFMRRGLAQSMQLRVVPNLHFHWDKSLENGD
                     RISRLLDQIERED"
     gene            6538..7521
                     /locus_tag="VFF70_15390"
     CDS             6538..7521
                     /locus_tag="VFF70_15390"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZY46121.1"
                     /translation="MPSSSSALNPIDPPVPLIDAIRHAQTILLITHLAPDSDAIGSLL
                     GLTHALRSIGKSIVPSCADQIFDRFNLLPGSQDIVQQTSGSFELIIALDCADASRMGN
                     IWAKLNDPKMPLINIDHHITNTQFGSINWVDPQATATSEIILTLIDQLNIQVTREIST
                     CLLYGIVGDTLGFRTPHTTPRSLEYSMRLMQAGAVLSEIMEQQFNRRSLALIKLWGKA
                     LNNLQIKDRIAFTTISKAMRTSVSMSGSSDISLSSFLISANEADRAAVLVETDDGQID
                     LSLRAKSGHDVAKAALALGGGGHPLAAGATIDGPMDVAVKRVIEALKANAA"
     gene            7594..8475
                     /gene="truB"
                     /locus_tag="VFF70_15395"
     CDS             7594..8475
                     /gene="truB"
                     /locus_tag="VFF70_15395"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZY46122.1"
                     /translation="MPDSGVLIVNKPRGLTSHDVINRVRRAIKIKQVGHAGTLDPLAI
                     GVLVVCINQATRISEYLMGHAKIYRARIRLGIETNTYDAEGELTATHDVKVTEAELRS
                     ALNHFVGPIDQIPPMHSAIKQGGQKLYDLARQGIEVDRPARSVTIHALDLINFESPDL
                     FIEVKCSAGTYIRSIAHDLGAQLGCGAQLIELQRTASGPYTIEQAIDLEQFETAARAD
                     HWEAHLHSIDEALSDWPSVTLNETDRLRALNGASIESITLSGSRCRAHDEHGNLIALL
                     MFDPKKSIWRADKVLKQ"
     gene            8517..9440
                     /locus_tag="VFF70_15400"
     CDS             8517..9440
                     /locus_tag="VFF70_15400"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZY46123.1"
                     /translation="MRLIHDLQEAQLTEESVVTIGAFDGLHIGHRKLIDGVRESAQSA
                     NRASVVVTFFPNPSIVLGRAEPFYLTSPEEKLVEINHTGVDLLVELPFTVDTAKTRAA
                     DFVDGLIQHLHMRELWIGHDFALGHNREGNADFLTRLGKERGYDVQLIEPLTNGNTVI
                     SSSTIRSALRVGDVAHAAKWLGRPFRLSGRVVKGDGRGRTIGVPTANLEMWPAHAIPA
                     NGIYACWAWVGHIRHRAAVSIGVRPTVTDNRIRTIEAYLLDFDKDLYGLNIALDFVAR
                     LRAEEKFSGLAELITQMKIDIALAREMLTNV"
BASE COUNT         2338 a         2577 c         2705 g         2010 t
ORIGIN      
        1 caccagcgct gatgtggtca cctcttttct gtacgatgcg gccaccgatc aattttttca
       61 tggcagcgct ttgggcggaa ccggtgaatg gactgacacc aaagcgattc gccgcgatgg
      121 catgacctac cgcgtgatcc aacaaggtca gccgattttg gtcggcgata ctttgcagga
      181 gcccgagacg aatcctggtt tgattcaatc cggcgtccga tcgttgatct gcgtcccatt
      241 gatcagcaaa ggcaaaccgc taggtgtctt attcgtcaac agtttcagcc cctacaaatt
      301 tgatgccgac gatgtgcaga tcgcctcagc gttggcgaat caagcagcca gcgccattga
      361 aaacgcgcgc ttgtttgccg atattgccga aggccgtgat aagctgcaag ccattctcaa
      421 cagcacgcat gacggcattt tgatgttcga tgcgtcaaca cggatcgtca ccgttaatcc
      481 acgcttggaa gaaatttggg acctgtcccg cgtcagtttg gaagaccgtt tgttgatcga
      541 tctgctcggt cagccggagt tccaaatcgc cgcgaaatta ggatttacgg ctgataagct
      601 gcatcatcaa ctgctacagg tgcaagcggg cgagcggatc gagtggccga aagaaattta
      661 tagtttgccc ggcagccggc cgcgcttcat cgaacgctcc ggtctgccgg tcctggatat
      721 agcgaaccgc ttgataggct ggatgctggt cttgcatgat gtcactgaag aacgcgagct
      781 gcagcaatcg cgtgatgatc tgagcaatat gatcgtccac gatttacgca gtccgttgac
      841 cagcatcttg ggcagtcttc agctgctgga ggaaaatatt caggagaagg acgcagcctc
      901 gatcgagcag caggccttag cgatcagcac gcgcagcacg cagaaactgc tggatctcgt
      961 caactcgctc ctggatatta ataaactcac cgcgggtcaa gtcaccatcg aatccgcacc
     1021 tgattcactg gccttaagca tcaacgcagc cattgagcgt ctatcggtgc tggccgtcga
     1081 ggaaggtgtc attatccgca aacaaatccc accggagttg ccgccggttc tgatcgatga
     1141 agagaagatt acgcgcgtct tgatcaattt gatcgacaat gccttgaagt tcagcccaca
     1201 cggcggacaa atcatggttc tcgccgaggt gtggccacac gacggcgctt cacctgcctt
     1261 cgttcgctgt gcagtgctag attcaggtcc cggtgtgccg cttgaatttc gcgagcgcat
     1321 cttcgatcga tttgtgcaag ttacccacca accaggccgg cggcacggaa ccggactcgg
     1381 cttaagtttc tgtcgattga cagtagaagc gcacggcgga aaaatctggg tcgatgatgc
     1441 gcccggcaaa ggcagcgtgt tttcatttac cctgccgatt gccaaggtct gacctcgctg
     1501 gttaatgccc atgccctacc attccacgtt ctgaagttac agccccatgt caaaactcgc
     1561 tgcgcgtctt gtcaaaaact agccgctatg ctataatctt aatcgcagtg cagttgatgc
     1621 agtcaagtgg aaagtgggca gcccccactt ttcttgttaa tatggcctac cattgatgaa
     1681 gcaacaaact tgcgagtttg tctaatccag gagcaagaga taaacaatga agagtgagtt
     1741 cctgctcgcc tttaaccaaa tatgcactga gcgaaacctg cccaaagaag tggttctgga
     1801 agcccttagc accgctctgg tcagcgccta tcggcggaat atcaacgcgt ccagtgcaca
     1861 aaatgtgacc gcggtgatcg atggcacgac cggcgtagcg cagatctacg ccgaaaaaga
     1921 agtggccgaa gatgtcaccg acgtgcgcac cgaagtgacc gtggccgctg cgcgccagat
     1981 tatgccggaa gccaaggtcg gcgatatggt catggtcgaa tcgacgccgg atggctttgg
     2041 ccgcattgcc gcgcagactg ccaagcaagt cattttgcag cgcatccgcg aagcagaacg
     2101 agacgcgcag tacaacatgt atgccgagcg cgaagaagag atcattaacg gcgtcgtgca
     2161 aagcattggt ccgagcggca ttactttaaa tttgggccgc actgaagcgt tgatgcccaa
     2221 gaaagagcaa gtgccgggcg aacgttatca aatgcaccag cgcttacgcg cctatgtcct
     2281 ggaagtccgc aagagcaccc gcggcccgca gatcattgtc agccgctcgc acaagaacat
     2341 gctgcgccgc ttgctcgaat tggaagtgcc ggaaattttc gccggaacgg tcgagattaa
     2401 atcgattgcg cgggaacccg gtgctcgatc gaagatagcg gtggccgcga ctcaggccgg
     2461 catcgatcca gtgggcgcgt gcgtaggcat gcgcggtgtg cgcatccaat cgatcgtcaa
     2521 tgaactgggc ggcgagaaga tcgacgtgat cgaatggaat ctcgatccgg cggcttatat
     2581 tcagaaagct ttgtcgcccg cgcgagtctt gacggttcat cccgatgatg atccacgcga
     2641 aggcaaaatt gccttagtcg tcgtgccgga tgatcaactt tcactcgcca tcggccgcga
     2701 aggccagaat gctcgactcg ccgccaaact taccggctgg cgcattgaca ttcaaggctc
     2761 caccgaatcg ctgcgcaagg ccttggctga gttggaggcc gatccggaaa ttagtgcgac
     2821 tatgggcgcg gatgcaactg cgattccaag cacacaagcc gcattgcaac gtcacactga
     2881 acaacagctg ccgtggaatc aagaagagat gcagtcgatc aagcacgtgc tggacggcgt
     2941 gcatcgtgta ctgaccgccc gcaagcaggc cgagcgtgag aggctgcgcg cacagattca
     3001 agccgaacgt gccattaagg cccaggaaga agcggtcgaa gtttcgcgca aagaacaact
     3061 ggccgcttac aaggcacgca ttcccgctca agcttatgag accatgctgg ccgatctggg
     3121 gatcagtgcg cgcgtcttga accatttgga aaagagcgat ctcaccaacg tcggtcagat
     3181 tcacgagcgc ttagtgagca gtgatgaatc gatgttgtcg attgaaggca tcggcccgaa
     3241 gggtctgact gaaatcaagc aggcgattga agagaagggg ctgggtttga tgcctctgcc
     3301 cgaacccgtc gtcatggaaa ccagcaccga agcaggcgcg ggacaaccga tcgagccagc
     3361 tcaaccgata ggggctgaag cagctgccgg agcagcaacc gccgaggccg tgtcgccaat
     3421 caaaccgatc gaaggtgcgc cggcgatcga actggaagaa gtcgacgacg acgacgatcc
     3481 cgataaaaag ctcaagaaga agggcaagcg caaagatcgc acgttggtgc tggacgagaa
     3541 gagcggcgtg gtggtggcga aaaagcagcg caagactggc cgcgccacag acattttcga
     3601 attgatcgac gaagaagaga tttaatcaat ggctcacgcc gccggcggcg gcgacgatcg
     3661 gtcggcgaga cgccgaccgg agcaggaatg tttatggccg gcaaacatat tccgcaacgg
     3721 acgtgtgtgg gttgccgcga agtgcagggc aagcggcaga tggtgagagt ggtacgccgc
     3781 gccgatgggc aagttagcgt ggatgcaacc ggcaaggcac ccggtcgcgg cgcgtatatc
     3841 catcaacgcc aaagttgttg gcagtacgct ttgaaaagtg gtcacttgac tcacgcctta
     3901 agagtgacac agttagccgc agacgatcgg ctggcgctgc aagcttatgc ggcggcgctg
     3961 ccgaaagatg aaactgaatc ttgagtgtcg tagttttgct cttcaactgc acgcaggcag
     4021 ttgattcgcc cgcagcgttg cggacggtga gcaagaagaa gatgaaaccg aagcttgaat
     4081 tagtgaccga tgcaatcgat tcttctcatg acttacagtt tgattgtggc ccagcgctgc
     4141 ggtgagtatg ccgcggcctg ctaactttat gaacccacaa atgttcccaa tcggactgta
     4201 acgcctcgct ggttgccagc ccacgacctg tgtgtgcgca ccaacgagtg atgcacggcg
     4261 tgcagcaatt tgacacgcgg tgaatcgatc gacggtcaac gcacggaggt gtggcatggc
     4321 agaagcagtt aagacttcca ccgtagttga aattcccagt gctttaacgg tgcgcgattt
     4381 agccgagcgg gtgaaggtca gcccgatcaa tatcatccgc gagttgatga acaacggcgt
     4441 catggccagc atcaatcagc agatcgatta tgacacggcc gcgatcgtgc tgcagggcat
     4501 gggctttgag acgcatgaag aagagcgtgc tgaagaaagc ccctcggaag atttgtccat
     4561 gacgctctgg cagcgtttat acgccaaaga agatccggcc aagctcgagg ctcgaccgcc
     4621 agtcgtcacc gtcatgggcc acgtcgatca cggcaagacc tcgctgctgg atgtgatccg
     4681 caataccagc atcgctgctg gtgaagcggg cggcatcacg cagcatatcg gcgcgtatca
     4741 agttgagcgt aatggtcgca agatcacctt cctcgatacg cccggccacg aagctttcac
     4801 ggcgatgcgc gcacgcggcg cgcaggctac ggacatcgca gtcttggttg tcgccgccga
     4861 tgatggggtc atgccgcaga ccaaagaagc gatcgcgcac atcaaagcag cgcatgtgcc
     4921 gattattgtg gcgctcaata agatcgataa atccaatgcg aatcccgaac gcgtcaagaa
     4981 agaattgaat gacaacggcg tcgtgatcga tgaatatggc ggcgatattt tgcttgtgcc
     5041 ggtttcggcc aagcaaaaga aaggcattga ggacttgctc gaagcgatct tgttggtagc
     5101 cgactcgcgc gagatcaaag ctaaccccga tcggtttgcc gtaggcactg tgatcgaagg
     5161 ccgcttggat aaatcgctcg gcgcggtcgc cacagtctta gtccaaaacg gaacgttgaa
     5221 agccggcgac gggattgtac tcggcaaagt ggctggccgc gtacgcacga tgtatgacga
     5281 caaaggcaaa gcgattcaga aagccggccc atcgacaccg gctttgatct cagggctgat
     5341 cggcgtgccc tccgccggcg atcaatttga agccgtcaaa gatgaaaaaa tcgccaaggc
     5401 catcatcggc gcaaagacga aagcggaagc gagcgatggc gttgtgcgca agaccttgac
     5461 attggccgat gtcttcagcc gcattcaagc cggtgaagtc aaagaactgc cgatcattct
     5521 caaagccgat gtgcaaggct cgctcgagcc ggtgatcaat caattggaaa aactcaacac
     5581 taccgccgtc aaggtcaagc tgttgcatag cagcaccggc acgatcggcg aaaacgatgt
     5641 gcagctggct atcgcctcgg gtgcgatcgt cgtcggcttc aacgtcaccg ccgatggatc
     5701 ggccaagcgt ctggccgatg cggaacacgt ggacgtgcgc acgtacgaca tcatttatcg
     5761 cttgactgac gatattcaaa aagctttaac cggcatgctg gaaccagttt acaaagaagt
     5821 cagcctcggc gtcgcggaag tgcggcaggt tttcaagatc aaaggcgtgg gcaacatcgc
     5881 cggatcgatg gtgcgcgaag gcgtcattca acgcggcgtc aaagcgcgcg tcaaacgcgg
     5941 cggcgaagtc gtgcacgaag gcggcgtcgg caccttgaag cggaacaccg aagacgtgaa
     6001 agaagtcaaa gccggtttcg aatgcggcat cggcttggca aacttccaag aattcaagaa
     6061 aggcgacttg attgaattct atacacagga acaagtgagt taacgcccag ctcaaggcgg
     6121 agcgtcagct ccgccgtctc atctgccgaa gctgacgctt cggcaagagc attgagagtc
     6181 agcatgacca cccgtcgaca agaacgcgtc gccgaacaga tcaagatcga actcggcaaa
     6241 atgttggagc atcagatcaa cgatcctcgc ttaaatctga tcagcatcac ggatgtgacc
     6301 atctcctccg atctgcgtga agcgaatgtc tataccagcg cgctggccgg tcaatctgtc
     6361 gaggccgaag tgcttgccgg cttggaacat gcgcgcagtt ttatgcgccg cggtttggcg
     6421 caaagtatgc agctgcgcgt ggtcccgaac ctgcattttc actgggacaa gtcgcttgaa
     6481 aatggcgatc gaatatcgcg cctgctcgat cagatcgaac gagaagattg atcgcccatg
     6541 ccgtctagca gttccgcgct taacccgatt gatcctcccg tgccattgat cgatgcgatc
     6601 cggcacgccc agactattct tttaatcacc caccttgcac cagatagtga tgcgattggt
     6661 agtttgctag gattgaccca cgctcttcga tcgatcggta aatcaatcgt gcctagctgc
     6721 gccgatcaaa tctttgatcg attcaatctc ctgcccggtt ctcaagacat cgttcaacag
     6781 acatccggct cattcgagct tattatcgcg cttgattgcg cagatgccag ccgcatgggc
     6841 aatatttggg cgaaactaaa cgatcccaag atgccgctca tcaacatcga tcatcacatc
     6901 accaacacac agttcggatc gatcaactgg gtcgatcccc aggccaccgc cacttctgaa
     6961 atcatcctca ccttaatcga tcagcttaac attcaagtca cgcgtgagat ttccacctgt
     7021 ttgttatatg gcatcgtcgg cgatacattg ggttttcgca caccgcacac gacgccgcga
     7081 tctttggaat actccatgcg cttgatgcaa gcgggcgcag tcttgtccga gatcatggag
     7141 caacaattca atcgccgctc gctggcgttg atcaaattgt ggggcaaagc cctcaataac
     7201 ttgcaaatca aagatcgcat tgcctttacg acgatcagca aagcgatgcg gacttcggtt
     7261 agcatgtccg gcagcagcga tatcagccta tccagtttct taattagcgc caacgaagcg
     7321 gaccgtgcgg cggtactggt cgaaacagat gatggccaga ttgatcttag tctgcgcgcg
     7381 aagagcgggc atgatgtcgc taaagccgcg ctagctttag gcggcggtgg acatccgctg
     7441 gccgccggtg cgacgatcga tgggccgatg gatgttgcag ttaagcgggt gattgaagcg
     7501 ttgaaggcga atgctgcatg atacgtaatg cgtgagggtt aagttgtatc atgcatcact
     7561 cgtcacgcat cacgttatta ctttccactt atcatgcctg attccggcgt cctcatcgtc
     7621 aataaaccac gcggcctcac ctcgcacgat gttattaacc gggtgcggcg cgcgatcaag
     7681 atcaagcaag tcgggcatgc gggcacgctc gatccgctgg cgatcggcgt attggtcgtg
     7741 tgcatcaatc aagcgacgcg catcagcgaa tatttgatgg gtcatgctaa aatctatcgc
     7801 gcgcgaatac gtttggggat cgagacgaac acgtacgacg cagagggtga actgacggcg
     7861 acgcatgatg taaaagtgac tgaagctgaa cttcgatccg cattgaatca ctttgtcggg
     7921 ccgatcgacc aaattccgcc aatgcattct gcgatcaaac agggcggaca gaaattatac
     7981 gatctcgccc ggcaaggcat tgaagtggat cgccctgccc gatcagtaac gattcacgcg
     8041 cttgatctga tcaactttga atcgcccgat ctgttcatcg aagtaaaatg ctcggcgggc
     8101 acgtacattc gatcgattgc gcacgatctg ggcgcgcagc tgggctgcgg cgcgcaattg
     8161 atcgaattgc agcgtacggc ctccggcccg tatacgatcg agcaagcgat cgatctggaa
     8221 caatttgaaa ctgcggcacg cgccgatcac tgggaagccc acctgcattc gatcgacgaa
     8281 gccttgagcg attggccgtc cgtcacgttg aatgaaaccg atcgactccg cgcgctcaac
     8341 ggcgcctcga tcgaatcgat cacactcagc ggctcgcgct gccgcgcaca tgacgagcat
     8401 ggcaacttga ttgcgctgct gatgtttgat cccaagaaaa gtatttggcg agcggataaa
     8461 gttctcaagc aataacctga caggccttaa tgacctgtca ggtttataat aggctcatgc
     8521 gactcattca cgatcttcaa gaagctcaac tcaccgaaga atccgtcgtg accatcggcg
     8581 cgttcgacgg cctgcacatt ggtcatcgca agttgatcga cggcgtacgc gaatccgcgc
     8641 agagcgccaa tcgcgcgtca gtggtcgtga cgttcttccc caatcccagc atcgtgttgg
     8701 gccgcgccga acccttctat ttgacttcac ccgaggagaa attggtggag attaaccata
     8761 ccggcgtcga tctgttggtt gaacttccct tcaccgttga tactgcaaaa actcgcgcgg
     8821 ccgattttgt cgatggcttg atccaacatt tgcacatgcg cgaattgtgg atcggtcacg
     8881 acttcgcttt gggccacaac cgcgagggca acgcggattt cttgacacgg ttaggaaaag
     8941 agcgtgggta tgacgtgcag ctgatcgaac ctttgacgaa tggaaacacc gtcatcagca
     9001 gcagcactat ccgcagcgcc ctgcgagtgg gcgatgtggc acatgctgca aaatggttgg
     9061 ggcgtccatt tcgattgagc ggacgggtgg tgaaaggcga cgggcgcggc cgaacgatcg
     9121 gtgtgccgac ggccaatctc gagatgtggc ccgcgcatgc tattccggcc aacggcatct
     9181 atgcgtgttg ggcgtgggtc ggtcacattc ggcatcgagc tgcggtcagc atcggcgtgc
     9241 ggccaaccgt gacggacaac cggattcgca cgatcgaagc ttatctgctc gattttgaca
     9301 aagacttata tggtttgaat attgcgttgg attttgtggc gcgtttacgc gccgaagaga
     9361 aattttcggg actcgctgaa ttgatcacac agatgaagat cgacattgcg ctggcgagag
     9421 aaatgttgac gaatgtataa tgcataattc ttaatgctta attgtgcagt atgcattcag
     9481 aataggatcc tattcatgaa gcaacgagac atttacctcc tcaaccccaa agaactcagt
     9541 cctgaagtga tcgccgtggc gtttgccaaa accagtcgat cgcccgaatc gttccgcgaa
     9601 atcgcggctg gattgacaga cgaaaaatca
//
LOCUS       DASRTJ010000763         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_223585,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000763 DASRTJ010000000
VERSION     DASRTJ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223585"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VFF70_15405"
     CDS             complement(<1..1129)
                     /locus_tag="VFF70_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HZY46124.1"
                     /translation="MIGLASAKPIKGKYLITPCSQMCNRGNGKGGGVAVVGCFPKFPN
                     HYAVQVGYLDLDARKEIEQKYIFPNFDVTGIEQQPYLEDFRDLPRLEIEPPRVVRYFS
                     RVKESILSKFAEEHGFKDMQAAEDEFVFQNSFRLNKEYYGSREDKKGFVLSHGRNMMI
                     LKAVGYAEDIAQYYQLEDMEANVWIGHQRYPTRGRVWHPGGAHPFSGLDEALVHNGDF
                     ANYFGVVEYLRQKNYFPLFMTDTEVAVYLFDLYNRVYGYPLEYVIEALAPTTERDFTK
                     LPIEKQPVYRAIQRSHLHASPDGPWFFIVARNDVQTQTMQLIGITDTSMLRPQVFALQ
                     EGERSIGVIASEKQAIDALLSDIAKDDPSVCPVADRYWNARG"
     gene            complement(1314..2822)
                     /locus_tag="VFF70_15410"
     CDS             complement(1314..2822)
                     /locus_tag="VFF70_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZY46125.1"
                     /translation="MTLSELSLLETLPIANTVSKWWVARSDACTHCNICVDVCPTGAH
                     KAADGYVRQPQPKSEVCIGPNCRSDGRNCVTECPADALRVESNQSYEVLGDARWPADL
                     LNATWHEAEYGVAPRNDLEWRVGRSGGGFDRLRFRVEDNLPEVLDPPGGLQAAYQQAL
                     SEVDLHLPLNRRHDEAEAITIDTPWYGGGMSYGSISLPVMLARASAAQRLGYFISTGE
                     GGYPIELSRYKDNVITQVATGLFGVREETLQWARIIEIKYAQGAKPGLGGHLLADKVT
                     RDVADVREAVEHTNLFSPFPFHSVYSVEDHKKHVDWLKAINPRAVIAVKVSTPEDVDM
                     VAVGSYYAGAHIIHLDGGYGGTGAAPDIAKKNIAMPIEYAIPKAHKFLVQEGIRDQVT
                     LMASGGLRTPYDVAKVIALGADGAIIGTAELVAVECVRCSNCESGRGCPKGIATTDAG
                     LTPIIGSHWGGQRIVNMYSAWLGELALLLKAMGLKSIAELRGRTDLLRYIKE"
     gene            complement(2877..>3916)
                     /locus_tag="VFF70_15415"
     CDS             complement(2877..>3916)
                     /locus_tag="VFF70_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZY46126.1"
                     /translation="TYSTHGRAPFTADILSALARQADEGRIPVTGAGYRGKFGGEGFD
                     GMWTDMSEIVRPTRDGIHGREYISTAIDLGRRSSRVLLNDQGQLAIDQAASLEIPIPI
                     VFQLPTIHADVIKVGMSIRHAARQLGTIVLDQSTHSEKLIQLDDSASLMEEIKHIKAD
                     HADTVIVVRLALRKDVADRVEQLTRSGAEVIHLAANWDGSTEDGSTLFDALPIVHQQL
                     VAANIRDEVTLLASGGIASAEHLPKTIILGADGVVIDLPLLAALECPLLDGCFEGHDC
                     TTPLNSIEVKWGRQRIVNLMAAWRNQLLEVMGAMGMREVRRLRGERGRAMFANELEEK
                     LFEPLFLMSNE"
BASE COUNT          884 a         1144 c          984 g          904 t
ORIGIN      
        1 caccccgggc gttccaatat cgatcggcaa ccggacagac cgaaggatcg tctttggcaa
       61 tatcggacag caacgcatcg atcgcctgct tctcggacgc gatcacaccg atcgatcgtt
      121 cgccttcttg caaggcaaat acttgcgggc gcagcatcga ggtatcggtg attccgatca
      181 actgcatcgt ttgagtctgg acatcgttgc gggcaacgat gaagaaccac gggccgtcgg
      241 gtgaggcgtg caaatgcgat cgttggatcg cccgataaac tggctgcttc tcaatcggga
      301 gtttggtgaa atcgcgctcg gtcgtcggcg ccagcgcttc gatcacatat tcgagcggat
      361 aaccgtacac gcgattgtag agatcgaaca agtagacggc cacttccgta tcggtcatga
      421 acaacggaaa gtaatttttc tgacgcaagt attcgacgac gccgaagtaa ttggcgaagt
      481 caccattgtg cactagtgct tcatcgaggc cgctgaacgg atgcgcgccg cccggatgcc
      541 atacgcgccc gcgcgtcgga tagcgctgat gaccgatcca cacattcgct tccatgtctt
      601 ccagttgata atactgtgcg atgtcttcgg cataaccgac cgctttgagg atcatcatgt
      661 tgcggccatg cgataagaca aaaccttttt tgtcctcgcg gctgccgtaa tattctttgt
      721 tcaagcgaaa agaattttga aagacaaatt catcttcagc ggcctgcata tctttgaagc
      781 catgctcttc agcaaacttg ctcaagatcg attctttgac gcgggagaaa taccgcacca
      841 cacgcggcgg ttcgatctca agtcgcggca gatcgcggaa gtcctccaaa taaggctgct
      901 gctcgatgcc agtcacgtcg aagtttggga aaatatattt ctgctcgatc tctttacgcg
      961 catccagatc gagataaccg acttgcaccg cgtaatgatt gggaaacttc gggaagcagc
     1021 caacgaccgc gactccgcca cccttgccgt tgcctcgatt gcacatctgg ctgcagggcg
     1081 tgatcaaata cttgcctttg atcggcttcg ctgatgccag gccaatcacg ccacaccctc
     1141 cttcggcttc ttccgattgt gacgagtgac gagtgatgag tgatgcgagt tcacttcgag
     1201 agttcaatat agtttcaggc atttcgcgca tagtcacctc aagttaacca cggagacaca
     1261 gtggccacgg agtattattt tattttctcc gtgttctcag tgtctcagtg gtttcattct
     1321 tttatatagc gtaacaaatc tgttcttcct cttaactccg caatgctctt caagcccatc
     1381 gcctttagta acaacgcgag ttcgcccagc cacgccgaat acatgttgac gattcgttgt
     1441 ccgccccaat gcgaaccgat gatcggcgtg agccccgcat cggttgtggc aatgcctttc
     1501 ggacaaccgc gacccgattc gcaatttgaa cagcggacac attcgaccgc gactaattcc
     1561 gcggtgccaa tgatcgcgcc atccgcgcca agcgcaatca ctttcgctac atcgtagggc
     1621 gtgcgcaagc cgccgctcgc catcaaggtc acttgatcgc gaatgccttc ttgcaccaag
     1681 aacttgtgcg ctttagggat cgcatactcg atcggcatgg cgatgttttt cttggcaatg
     1741 tccggcgcgg cacccgtacc gccgtagcca ccatcgagat gaatgatgtg cgcgcccgcg
     1801 taatacgatc ccacggcaac catatcgaca tcttcgggcg tcgagacttt caccgcaatc
     1861 accgcgcgcg gattgatcgc ctttagccaa tcgacatgct tcttgtgatc ttcaacggaa
     1921 taaaccgaat gaaacggaaa cggcgagaac aaattcgtat gctcgacggc ttcacgcaca
     1981 tcggcaacat cgcgcgtgac tttatccgcc agcaggtgtc caccgaggcc gggcttcgcg
     2041 ccctgcgcgt atttgatctc gatgatacgc gcccactgca aagtttcttc gcgcacgccg
     2101 aacaaacccg tcgcgacttg ggtaatgaca ttatctttgt agcgcgacaa ctcgatcgga
     2161 tagccgccct caccggttga gatgaaatag cccaagcgtt gagccgcaga cgcgcgcgcc
     2221 agcatcaccg gcaacgagat cgagccatac gacatgccac cgccatacca cggcgtatcg
     2281 atcgtgatgg cttcggcttc gtcgtgccgc cgattgagcg gcaagtgcag atcgacttcg
     2341 ctcaaggctt gttgataagc cgcttgcaat cctcccggag gatccaaaac ttccgggagg
     2401 ttgtcctcaa ctctgaagcg caatcgatcg aacccgccgc ccgatcggcc tacgcgccat
     2461 tcaagatcgt tacgcggcgc gacgccatac tcggcttcat gccaggtggc attcaacaaa
     2521 tctgcaggcc agcgcgcatc gccaagcact tcgtaacttt gatttgattc aactcgcagc
     2581 gcatctgccg gacattcggt cacacagttg cggccatcgc tgcggcaatt tgggccgatg
     2641 cacacttcgc ttttcggctg cggctgacgc acatatccat ccgccgcttt gtgcgcaccg
     2701 gttggacaga catcgacgca gatgttgcag tgcgtgcatg catccgatcg cgcgacccac
     2761 catttggata cggtgttggc gatagggagt gtctctaata aagacaattc gctaagtgtc
     2821 atgcgttgct ctcctgataa cgagtaatga gtaacgagta aatcttgtct tactcattac
     2881 tcgttactca ttaagaatag tggttcgaat aacttttctt ccaactcatt cgcaaacatc
     2941 gcgcggccac gctcaccgcg caagcggcga acttcacgca tgcccatcgc gcccatcact
     3001 tccagcaact gattgcgcca cgcggccatc aggttcacaa ttctttgccg tccccacttc
     3061 acttcgatcg aattgagcgg cgtggtgcag tcgtgtcctt cgaaacaacc atccagcaac
     3121 ggacattcta aagcggcgag caatggcaaa tcaatgacga cgccatcagc gccgagaatg
     3181 atcgtcttgg gcaaatgttc cgccgatgcg attccgccac tggccagcaa agtgacttca
     3241 tcacgaatgt ttgccgccac caattgctga tgcacgatcg gcagcgcatc gaacaacgtc
     3301 gatccgtctt cggtcgatcc gtcccagttg gcggccagat ggatcacctc tgcacccgat
     3361 cgagttagct gctcaacgcg atcggccaca tcctttctta acgccaaccg cacgacgatc
     3421 accgtgtccg catgatcggc tttgatgtgt ttaatctctt ccattaagga agcagagtca
     3481 tcaagttgga tcagtttttc tgaatgagtg gattgatcta aaacaattgt gcccaactgc
     3541 cgggccgcat gacggatcga cataccgact tttatcacgt ccgcatgaat cgtcgggagt
     3601 tggaagacaa tcggaattgg gatttctaaa gaggctgctt gatcaatcgc caattgacct
     3661 tgatcattca acagtacccg tgacgatcgg cggcccagat cgatcgcggt gctgatgtat
     3721 tcgcggccgt gaatgccgtc gcgcgtcggt cgaacaatct cgctcatatc ggtccacatg
     3781 ccatcgaaac cttcgccgcc gaacttcccg cgatagcccg cgcccgtcac ggggatgcgg
     3841 ccttcatcgg cttgacgtgc gagcgctgac agaatatccg cagtaaatgg cgcgcggccg
     3901 tgcgttgagt aggtcg
//
LOCUS       DASRTJ010000764         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_223591,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000764 DASRTJ010000000
VERSION     DASRTJ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223591"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFF70_15420"
     CDS             complement(<1..642)
                     /locus_tag="VFF70_15420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZY46127.1"
                     /translation="MNRKIWIALIGYLMIGAAACQSSAATPLPSGATTVQSQPVPAGD
                     DNLTATASGLKYADLIVGSGGTPAATDYVTVQYTGTLDNGTVFDASRQHGGPAEFPLD
                     QVIPGFTEGVGSMKIGGTRKLVIPPNLAYGSQANGPIPANSTITFIVELISVKPAPQV
                     KVEDLVVGTGAEANVGSTLTVNYTGTLENGTVFDASYGKSPFDFQLGAGQVIPG"
     gene            complement(711..1145)
                     /locus_tag="VFF70_15425"
     CDS             complement(711..1145)
                     /locus_tag="VFF70_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46128.1"
                     /translation="MNLRLDKVIRNTGFSVRKVYSLVLSHEALYIIHTGSIGALKHYH
                     FDTATQRVVADASNDRSVAEIQSNETQIDLTLLDQLVGGNNYRVHLVAIEDVSVRSGQ
                     LPEMIVKVTGSDHRLIFPFTPIDQVLTLRAALLRQDDQVASR"
     gene            complement(1142..>1700)
                     /locus_tag="VFF70_15430"
     CDS             complement(1142..>1700)
                     /locus_tag="VFF70_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46129.1"
                     /translation="LISLGFAFVTLAWNDLNKIKGLVSAHPFLYISACLYLIGLPIYV
                     FGSIMRRPRQPLTPRAQTRSALAPWSAVWRAAQALMDFFDSLITIVFAVVFTLLAFAW
                     LICVAPVQYFVYLICAAPARLMRRSRRRVISNFDEAQYFHLDEVPAEGEAPAGWLEAG
                     FFTRPVSMTAAFAAALLWILAQTIR"
BASE COUNT          419 a          464 c          451 g          366 t
ORIGIN      
        1 gcctgggatc acttgacctg caccgagctg gaaatcgaac ggcgatttac catacgatgc
       61 atcaaagacc gtgccgttct ccaacgtgcc cgtgtaattg accgtcaacg tgctgccgac
      121 attcgcttca gcgccggtgc ccaccaccaa gtcttcgacc ttcacttgcg gcgcgggctt
      181 gacgctaatg agttcgacga tgaaagtgat tgtggagttt gctggaatcg gaccattggc
      241 ctgactgccg taagccagat tcggcggaat aactaacttg cgcgtgccgc cgatcttcat
      301 cgagccaaca ccttcggtaa aacccggaat gacttgatcg agcggaaatt cagccggccc
      361 gccatgctga cgcgaagcat caaacacagt gccgttatcc agtgtgccgg tatattgaac
      421 agtgacgtaa tcggttgcag caggtgtgcc accagacccg acaatgagat cggcgtactt
      481 gagtccggat gctgtcgcgg taaggttatc atcacctgct ggcactggtt ggctctggac
      541 agtggtggcc ccgctcggca atggcgttgc tgcagatgat tgacaagccg ctgcgccgat
      601 catcaagtaa ccaatcagcg cgatccaaat tttgcgattc ataaacttag tccttttaat
      661 ttattttggt tcttagccaa ggccggtaat catagcatac cgccgcgctt tcagcgagac
      721 gctacttgat cgtcctgccg cagcagtgcc gcacgcagcg tcaacacttg atcgatcggc
      781 gtaaacggaa atatgaggcg atgatcactc ccggttactt tgacgatcat ttcgggcagc
      841 tgaccggatc gcacgctgac atcttcgatc gctaccaaat gtacccggta attgttaccg
      901 ccaacgagtt gatcgagcag tgtgagatcg atctgtgttt cgttcgattg gatctctgcg
      961 actgatcgat cattcgaggc gtccgccacc acccgctggg ttgctgtgtc aaaatgatag
     1021 tgcttcagcg ccccgattga tccggtgtga ataatataca acgcctcatg gctcagcacc
     1081 agcgaataga ctttgcgtac actgaaaccg gtgttgcgaa tcaccttatc aagtcgaaga
     1141 ttcatcgtat cgtctgggcc aaaatccaca gcagcgccgc cgcaaatgcg gctgtcattg
     1201 aaacgggtcg cgtgaagaag ccggcctcca gccagcccgc cggcgcttca ccctccgctg
     1261 gaacttcatc gagatgaaaa tattgtgctt cgtcgaaatt tgaaatcacg cggcggcgcg
     1321 atcggcgcat cagcctcgcg ggcgcggcgc agatcaaata aacaaaatac tggacgggtg
     1381 caacacagat caaccaagcg aaggcgagta gtgtaaacac taccgcaaag acgatcgtga
     1441 tcagtgaatc aaagaaatcc atcaacgctt gagcggcacg ccagactgcg ctccagggcg
     1501 ccagcgctga tcgggtttga gcacggggcg ttaacggctg ccgggggcgg cgcataatcg
     1561 atccgaaaac atagatcggc aggccgatca aatacaagca agcgctgata tagagaaatg
     1621 gatgtgcgga gaccaaacct ttgatcttgt tgagatcatt ccaggccagc gtcacaaacg
     1681 caaagcctaa actaatgagt
//
LOCUS       DASRTJ010000765         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_223768,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000765 DASRTJ010000000
VERSION     DASRTJ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223768"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFF70_15435"
     CDS             complement(<1..772)
                     /locus_tag="VFF70_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46130.1"
                     /translation="MKSKIQNPKSKILWLVIPALIFVIAAWFDLSPYLRGPDEWRWTL
                     RSIQSPERLLVPIAVLGLYGLISASCLNRSAISAKKFLIFVTIAAPIIQLGLAFAVSR
                     YPLLEFFGPTVSVHNSGYFTTAIANNDLNNLLSNYPQLMPSLPIHAQSHPPGPIVAHW
                     LGWKFFQALPPLADSLAMPLRTLQCHNPGLMALDNPQIASALIGMLIPLIGALAVWPM
                     YALGKRIIGSPAALISVALFPIMPLFAMWPTQWDQIYPL"
     gene            complement(769..2094)
                     /locus_tag="VFF70_15440"
     CDS             complement(769..2094)
                     /locus_tag="VFF70_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46131.1"
                     /translation="MRRSFGAILFLLFMIVWALFTKIDTHSWQEESRMATIQTLVEQN
                     TFAIDHTEFNRTGDKVFVSDHFYSDKTPLLSVVAAGGYSILHNSFYLTLDPTICVPDE
                     DVAACRVFDGKNFRFTAFYWLTLIFIGGSSALLVTLSWRAMLDTGAGGILSTALALAV
                     GLASPIAPYSIVFAGHVPAALCLFAGFFLFVRCHSERGDESLSQIYRKDNRNLFFAGL
                     LISLSANIDLTLAVFIAAFGLWILLARRNNFLSYLIGAIVPFAVSAAINYWAAGSIMP
                     LYFDPNAYDFFGTVLNKSVGGTNGFYSLDFGLRYTYDLLIGARGLFSFTPMLIFAIVG
                     LWIVLRDRANRLRGLTFAVIAGSLTFTLYLIFRTDNFGGEAWGTRWIVPLAPMLMYYA
                     GNAYRISALTYRGALVWRIVFWLLVILSFATAVYGLHDAWKSVPPIVRL"
BASE COUNT          655 a          572 c          608 g          412 t
ORIGIN      
        1 acagcggata gatttgatcc cactgggtcg gccacattgc aaaaagcggc atgatgggga
       61 acaaggcaac agaaatcaag gcggcaggtg agccgataat ccttttaccc aatgcataca
      121 tcggccaaac agccagcgcg ccgatcaagg gaatcaacat accaatcaac gcgctagcga
      181 tctgcggatt atcgagcgcc atcaagccgg gattgtggca ctgcaatgta cgcagcggca
      241 tggcgagcga atcagccagc ggcggcaatg cttgaaagaa tttccagccg agccagtgcg
      301 ccacgatcgg gccgggcgga tgcgattggg cgtgaatcgg cagcgagggc atgagctgcg
      361 ggtagttgct cagcaagtta ttcagatcat tgttcgcaat tgccgtcgtg aagtagccgc
      421 tgttgtgaac agaaactgtc ggtccgaaaa attccaagag tggataacgt gaaaccgcaa
      481 acgctaatcc gagttgaatg atcggcgcag cgatggtaac gaaaatcaaa aactttttcg
      541 ctgagatggc cgatcgattc aagcagctcg cggagataag cccatacaag cctagcaccg
      601 caatcggcac aagcaagcgc tccggagatt ggatcgatcg caatgtccaa cgccattcgt
      661 cggggccacg cagatacggg gagagatcga accacgccgc aatgacgaag atcagtgcag
      721 gaatgaccag ccacagaatt ttagattttg gattttggat tttggatttc ataagcgaac
      781 gatcggtgga acagatttcc aagcatcatg taagccatat accgcagtcg caaatgagag
      841 gatgactaat aaccaaaaaa caattcgcca tacaagcgca ccgcgatagg taagagccga
      901 tatgcgatac gcattgcccg cataatacat caacatcggc gcaagcggca cgatccagcg
      961 tgtgccccag gcttcgccgc cgaaattatc cgtgcggaaa atcaagtaga gggtgaaggt
     1021 caaactgcca gcgatgacag cgaacgtcaa tccgcgcaag cggttcgctc gatcgcgcag
     1081 gacaatccac aagccaacga tcgcgaagat caacatcggc gtgaaagaga ataaaccgcg
     1141 cgcgccgata agcagatcgt atgtgtagcg taatccaaaa tcaagcgagt agaaaccatt
     1201 cgtgccgccg acggacttgt tcaagactgt gccgaagaag tcataggcgt tcggatcaaa
     1261 gtacagcggc atgatcgagc cggcggccca ataattgatc gccgcgctga cggcaaacgg
     1321 aacgatcgcg ccgataagat aagagaggaa gttgtttcgg cgtgcaagta agatccacag
     1381 tccaaatgcg gcgatgaata ctgcgagagt gagatcgatg ttggccgaga ggctgatcaa
     1441 caatccggcg aaaaataaat ttcgattatc cttgcgataa atttgcgata gtgattcatc
     1501 gcctcgttcg gagtgacagc gtacgaatag gaaaaaacct gcaaacaggc acagcgccgc
     1561 cggaacatgc cccgcaaata cgatcgagta cggcgcgatc ggcgaggcaa gacccacggc
     1621 cagcgccagc gcagttgata agatgccccc tgctcccgta tcaagcatcg cccgccaaga
     1681 cagtgtcacc agtaaagcag atgatccacc gatgaagatg agcgtcaacc aatagaatgc
     1741 ggtgaagcga aaattctttc cgtcaaaaac gcggcaagct gccacatctt catcaggcac
     1801 acaaattgtc ggatcgagcg ttaaatagaa agagttgtgc aggatcgaat atccgccagc
     1861 ggccacaacg gacaacagcg gtgtcttatc ggagtagaaa tgatcgctga caaacacttt
     1921 atcgccagtg cgattgaact cggtatgatc gatggcgaat gtattctgct cgacgagcgt
     1981 ttgaatcgtg gccatgcgcg attcttcctg ccacgaatgc gtatcaatct tagtgaacaa
     2041 tgcccagacg atcatgaata gcaaaaacaa gatcgcgccg aatgatcggc gcatgaggaa
     2101 cgcatccagg cggctcaggg ctgcacctgc acttgagtca agaaaattcg atcggcagca
     2161 ggttgatcgc caacctcgat cggcaaccgc tgcccggtgt ctctatgata caagcccaca
     2221 ccgatccaat atgatccggc cggggtg
//
LOCUS       DASRTJ010000766         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_224373,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000766 DASRTJ010000000
VERSION     DASRTJ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224373"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFF70_15445"
     CDS             <1..1041
                     /locus_tag="VFF70_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZY46132.1"
                     /translation="DFLNARRPEEISFGQNMTSLTLHISRSIARTLKPGDEIVVTRLD
                     HDANITPWTLIAEDRGATVKWVDFDPEDCTWSVDDLKHQINSKTKLVAVGYASNAVGT
                     INHVAEAVQAAHAVGALCFVDAVQYAPHGPIDVQALDCDLLACSAYKFFGPHTGILYG
                     KYELMNDLKAYKVRPSGNEPPDKFETGTQSFESIAGMLGALEYFEWLGETFGAEFEAQ
                     YAPYNGRRRAFKQAMAVMREYEFDLSRKLIEGLSLIKGLHVWGITDPKQMAQRVPTVS
                     FTLNGWQPRQVAEELAKANIYVWDGNYYALAVMERLDLENKGGMVRVGAAHYNTFDEV
                     DRLVEAVKKLAK"
     gene            1169..1528
                     /locus_tag="VFF70_15450"
     CDS             1169..1528
                     /locus_tag="VFF70_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZY46133.1"
                     /translation="MEKVNIAQKFSLFNDHWSPKIVGELNDTHVKLVKLKGEFVWHHH
                     ETEDELFLVIKGQLLIKLRDQDIQLDEGEFAIIPKGVEHLPIAAEECYVLLLEPKTTL
                     NTGTVQNDRTVVDLQRI"
     gene            1540..1938
                     /locus_tag="VFF70_15455"
     CDS             1540..1938
                     /locus_tag="VFF70_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZY46134.1"
                     /translation="MTARQNISSGTKWEPFVGYSRAVKIGSHVYVSGTTATDADGKIV
                     GKDDPDAQTVQTLKNIEAALLRADASLKDVVRTRMYVVNIDDWEKIGRAHGEFFGDVR
                     PASTMVEVKRLIDPEMLVEIEAEAYIDEEN"
     gene            1943..2335
                     /locus_tag="VFF70_15460"
     CDS             1943..2335
                     /locus_tag="VFF70_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZY46135.1"
                     /translation="MTRQSVSTGSPYEPIFGIARAVRIGNTIAVAGTAPLGPDGKTVS
                     IGDAAGQMRRCIEIAQIALEKLGASLSDVIRTRTLLTRIEDWEVTGRVHGEVFKDIRP
                     VNTVMQVTRFIDPEWLVEIEVDAVVDEN"
     gene            2408..2983
                     /locus_tag="VFF70_15465"
     CDS             2408..2983
                     /locus_tag="VFF70_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZY46136.1"
                     /translation="MPKDYNLEATPANQQRRNELVQDDEWINEFLQRASIGHIATRWD
                     EQPFITPTTYWYDGARHEIYFHSNVVGRIRANSEHHDRVCFEASEFGKLLPSNVALEF
                     SIQFESVIAFGSIRVLEDAEEKRFALSNLLGKYFPQMTAGKEYRPITDEELKRTSVYA
                     IAIESWSGKRNWPEQADQGDEWLPLIAAQAN"
BASE COUNT          793 a          747 c          888 g          679 t
ORIGIN      
        1 gattttttga acgcccgccg gccggaagag atcagcttcg ggcagaacat gacttcgctc
       61 acgctgcaca tcagtcgatc gatcgcacgc accttgaagc cgggcgatga gatcgtggtc
      121 acgcgcctgg atcacgatgc caacattaca ccgtggacat tgatcgccga agatcgcggt
      181 gcgactgtaa agtgggtgga tttcgatccg gaagattgca cgtggagcgt cgatgatctc
      241 aagcaccaga tcaattcaaa aacaaaactg gtggcggtgg gttacgcttc gaatgcggtc
      301 ggcacgatca atcatgtggc tgaagccgtg caagccgcgc atgccgtcgg cgcgttgtgc
      361 tttgtcgatg ccgtgcagta tgcgccgcat ggcccgatcg atgtccaggc gctggattgc
      421 gatctcctcg cttgctcggc ttacaaattc ttcgggccgc ataccggcat cttatatggc
      481 aaatatgaat tgatgaatga cttgaaggct tacaaagtcc ggccgtcggg caatgagccg
      541 ccggataaat tcgaaaccgg cacgcagagc tttgaatcga tcgcgggcat gctgggcgcg
      601 ctggaatatt tcgaatggct gggtgaaacc ttcggcgcgg aattcgaagc gcagtacgcg
      661 ccctacaacg gccgccgccg ggcgtttaaa caagcgatgg ctgtcatgcg cgaatacgaa
      721 ttcgatctca gccgcaagtt gatcgaagga ctttcattga tcaagggact gcatgtgtgg
      781 ggcattaccg atccaaaaca aatggctcaa cgggtcccga ccgtttcgtt cacgctcaac
      841 ggttggcagc cgcgccaagt ggctgaagaa ctcgctaaag ccaacatcta tgtgtgggac
      901 ggaaattact acgccttggc tgtaatggaa cgcttggact tggaaaacaa aggcggcatg
      961 gtgcgcgtcg gcgcggcgca ttacaacacc ttcgacgaag tcgatcgatt agttgaagcc
     1021 gtgaagaaat tggcgaagta aacatttcgc gcgtcattgc gaggagcgtt ttttgcgacg
     1081 aagcaatccc cggatcgacg ctgagattgc ttcgccgcgt agcggctcgc aatgacggac
     1141 gacattttcc gttaaggggg aatcaatgat tgagaaagtc aacatcgcgc agaaattcag
     1201 tttgttcaac gatcattgga gtccgaagat cgtcggcgag ctgaacgata cacatgtcaa
     1261 gctggtgaag ctcaaaggtg aatttgtctg gcatcatcac gaaaccgaag acgaattatt
     1321 tctggtgatc aaaggtcagc tcttgatcaa attacgcgat caagatattc aacttgatga
     1381 aggcgagttc gcaattattc caaagggcgt cgagcatctg ccgatcgctg ccgaagaatg
     1441 ctatgtgctg ttgctcgaac ccaaaacaac gttaaatacg ggcaccgttc agaacgatcg
     1501 aacggtggtt gacctgcaaa gaatttaatc ggagggacga tgactgcgcg ccaaaacatt
     1561 tcatctggaa caaaatggga accattcgtc ggttattcgc gcgccgtcaa aatcggatcg
     1621 catgtgtatg tgtcgggcac gactgcgact gatgcggatg gaaagatcgt cggcaaagac
     1681 gacccagacg cgcaaacagt ccaaaccttg aagaacatcg aagcggcatt gctgcgcgca
     1741 gacgcgagtc tcaaggatgt cgtgcgtacg cggatgtatg tagtcaacat cgatgactgg
     1801 gagaagatcg gtcgggcgca cggcgaattc ttcggcgatg ttcgacctgc atcgacgatg
     1861 gtggaagtga agcgtttgat cgatcctgag atgctggttg aaatcgaagc ggaggcttac
     1921 attgatgagg agaattaatc aaatgacacg ccaatctgtt tccactggat cgccttatga
     1981 accgattttc ggtatcgcgc gcgcggtgcg catcggcaat acgatcgccg ttgcgggcac
     2041 cgcgccactt ggcccggatg gcaaaaccgt ttcgatcgga gacgcggcgg gccaaatgcg
     2101 gcgctgcatc gagattgctc agattgccct ggaaaaatta ggcgcgagtt tatcggatgt
     2161 gatccgcacc cgtacgttat tgacgcgcat cgaagattgg gaagtcaccg gtcgagttca
     2221 cggcgaagtc ttcaaagaca ttcggccggt gaatacagtg atgcaagtga cgcgcttcat
     2281 cgatccggag tggctggtgg agatcgaagt cgatgcggta gttgacgaga attaaaccgc
     2341 caagcccgcg agaggcgcaa agatcttttg tctttctttg cgcaccttgc ggtcagaaat
     2401 gacaatcatg cccaaagatt ataatctgga agcaacaccg gcgaatcaac agcgccgcaa
     2461 tgaattagtg caagacgatg aatggatcaa tgaattcttg cagcgcgcct cgatcggcca
     2521 catcgctacc cgctgggatg agcaaccttt cattacgccc acgacgtatt ggtatgatgg
     2581 cgcacgccac gaaatctact ttcactcgaa tgtcgttggc cgcattcgag cgaacagtga
     2641 acaccacgat cgagtgtgct ttgaggccag cgagtttggc aaattactcc cgtcgaatgt
     2701 ggcgttggag ttcagtattc aatttgaaag tgtgatcgct ttcggatcga tccgcgtatt
     2761 ggaggatgcg gaagaaaaac gatttgcctt gagtaacttg ctgggcaaat attttccgca
     2821 gatgacagcg ggcaaagaat atcggccgat taccgacgaa gagttgaaac gcacgtcggt
     2881 ctatgcgatc gcgatcgaga gttggagcgg caaacgcaat tggccggagc aagccgatca
     2941 aggagatgag tggctgccac tgatcgcggc gcaagcaaat tgacacgccg ttccaagaat
     3001 taggattgat aaaacgaacg tgggacaaat tgagaagccg tcagctgttg acaggcaaat
     3061 cttgtcccac gttctgtggt tactgcaggt gaaaacctca attgttc
//
LOCUS       DASRTJ010000767         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_224615,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000767 DASRTJ010000000
VERSION     DASRTJ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224615"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VFF70_15470"
     CDS             <1..1348
                     /locus_tag="VFF70_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46137.1"
                     /translation="IFDNEAGYRSFLGADPRSAYKLRSVCDRTIEMTESHVMSIDLQA
                     IESSIEQNLAEGYSKKNKMEDQPPRGLPMRTGDARAAIAFGGGRFHPQMIAWVASLVS
                     DLIKQQHVEPRDIVILAPFLSDSLRFALQDRFDRQSIPTRSLRPSRALNEESITRGLM
                     TLSTIAHPDWHLAPPQSDVAQMLVMSIAGLDPARAHLIAERLYRSRDGQIELRAFDGL
                     KPELQDRIGYEIGAKFENIRAWIADRVQSETALPLDHFLSKLFGELLSQYGFGFHHQI
                     DPGRITAQIIESARKFRQTVSPDPFAAMSDPSIGRDYIRMVAEGIVAATFIPSPYADI
                     PNAVLLAPAYTFLLSNESVAYQFWLNAGSPAWWERLYQPLTHPYVLSRNWPIDKKWTD
                     EDEQLARRATLLRLLRGLVRRCRLKIYLAMSTLNEQGFEERGPLLSIVQQVLREIR"
BASE COUNT          358 a          416 c          396 g          346 t
ORIGIN      
        1 catttttgat aacgaagccg gctatcgatc gtttttgggc gcggatcccc gatcggctta
       61 taaactccga tcggtgtgcg atcgaacgat cgagatgact gagtcgcatg tcatgtcgat
      121 cgatctgcaa gcgattgaat catcgatcga gcaaaacctc gcggagggtt actccaagaa
      181 aaacaaaatg gaagaccaac ctccgcgagg tttgcctatg cgaacgggcg atgcacgcgc
      241 ggccattgcg ttcggcggag gacgttttca tccacagatg atcgcctggg ttgcatcact
      301 agtgagcgac ttgatcaagc agcagcacgt tgagccgcgc gatatcgtca tcctcgcacc
      361 gttcttaagt gactcattgc gcttcgccct gcaagatcga ttcgatcgac aatcgatccc
      421 gacacgatca ctgcgaccat cgcgcgcgct gaatgaagaa tcgattacac gcggcttgat
      481 gacgctgtca acaatcgctc accccgattg gcatctcgct ccgccgcaat ctgatgtggc
      541 ccagatgctg gttatgtcca tcgctggact cgatccggcg cgcgcgcatt tgatcgccga
      601 acgcttatat cgatcgcgcg acgggcagat cgaattacgc gctttcgacg gcctgaagcc
      661 agagctgcaa gatcgcatcg gctacgagat cggcgcaaag tttgaaaaca ttcgggcctg
      721 gatcgccgat cgggttcaat ctgaaaccgc actgccttta gatcattttc tcagcaaact
      781 tttcggcgaa ctattatcgc aatacggctt tggctttcat catcagatcg atccgggccg
      841 catcacggcg cagatcatcg aatcggcgcg caagttccgg caaaccgtca gccccgatcc
      901 atttgcggcg atgagcgatc cgagtattgg ccgcgattac attcgcatgg tggccgaagg
      961 gattgtcgcg gcgaccttta ttccaagccc ttatgctgac ataccgaacg cggtcttatt
     1021 ggctccggct tatacttttt tactcagcaa cgaatcggtg gcttatcaat tttggttgaa
     1081 cgccggcagt ccggcgtggt gggaaagact gtatcaaccc ctcacacacc cgtacgtact
     1141 gagccgcaac tggccgatcg acaagaagtg gaccgacgaa gacgagcaac ttgcacggcg
     1201 cgcaacgctg ctgcgactct tgcgcggact ggtgcgccgg tgtcgattga aaatctattt
     1261 agcgatgagc acgcttaacg agcaaggctt tgaagaacgc ggccccctgc tctcgatcgt
     1321 gcaacaggtg ctacgcgaga ttcgctagcg caagtataac ggatagagta gaatcaattc
     1381 gtcagacatt taccaacttg ctgcaatacg atcggatcat gccgcgacac gaaggaagta
     1441 ccagtgcctt cgtgttttta tgtgtcatgc acagtcaaaa ttatgaactc tgaattctcg
     1501 attcaactca aatggg
//
LOCUS       DASRTJ010000768         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_225570,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000768 DASRTJ010000000
VERSION     DASRTJ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225570"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFF70_15475"
     CDS             <1..1124
                     /locus_tag="VFF70_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZY46138.1"
                     /translation="VETLAIPLPDLLPRSAKFVQAHITGFDLDQHVVKTDHGDFPYVR
                     LIIALGSQSATYNIPGLNEHTLMLKSVQDARHIRGHFEIVLSGLKNRSSSMPYSIVIG
                     GAGITGVELAAELSEGLAALLREYELGRDAVKVILIEASATVLPGFDHKTIDEAAQSL
                     ACLGVEVRTGTIVERVEANRVIVKPIGFAVLEEIETETLIWTGGIRANSLIAHSGLKI
                     GERGAAIVDEYLHAIDQHEVYVLGDSAVVRDPRDGHIATPCGQLAVKQGQYAAKDILA
                     DVKGDIHRPYIPYMDGLLISLGSYAGVGTIGPVWVRRLIARLAKIGAETRYLYNVGGV
                     RLVSSRWLGLRHEWVALNRGLRNLLGHRRSLRANHTAVK"
     gene            1488..2399
                     /locus_tag="VFF70_15480"
     CDS             1488..2399
                     /locus_tag="VFF70_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZY46139.1"
                     /translation="MIKRIVIPGGSGFLGQALIDHWAARDYEIVVLTRSSTATNSQAR
                     FIQWDGKTLGDWSAWLEGAEAIINLTGKSVNCRYTPKNRNEIIASRVNSVNAIGQAIQ
                     RCTQPPNVWIQASSLAIYGDAGDRICDESAPYGQGFPVETCIVWEQALAALSLAHIRT
                     VALRIGFALNRSGGAFGPLKNLAKFFMGGTAGSGKQYVSWIHINDLNRMFDWCLERDD
                     LSGVFNATSPRPMTNAEFMRAIRHVLHRPWSPPIPSWAVQFGSIILRTEAELVLRGRR
                     CIPKRFSDLDFKFEFPELIPALQNLTA"
     gene            2670..>2970
                     /locus_tag="VFF70_15485"
     CDS             2670..>2970
                     /locus_tag="VFF70_15485"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HZY46140.1"
                     /translation="MPDIDEQIQKAQQAGEFDRLRGKGKPFTHLDSDPLRHLLDAQGF
                     TPHWLEADHEIQRKIELAEQAIKRTYEWVMELWSSGSADRHFAREEWRRALKIFDE"
BASE COUNT          730 a          707 c          863 g          670 t
ORIGIN      
        1 atgttgagac gctggcaatc cctctgcccg atttgctgcc gcgctcggcg aaatttgtgc
       61 aggcgcacat cacaggtttc gatctcgatc agcatgtggt caagaccgat cacggcgatt
      121 tcccttacgt tcggttgatc attgcactcg gcagccaatc ggccacatac aacattccgg
      181 gcttgaatga acacaccctg atgttgaaat cggtgcagga tgcgcgtcac attcgcggtc
      241 acttcgaaat cgttttgtcc ggattaaaaa accgatcgag ttcaatgcct tactcgatag
      301 tgatcggcgg cgcgggcatc accggtgtgg aactcgccgc ggagttaagt gaaggtctag
      361 cagcgttgtt gcgcgaatat gaactcggac gtgatgcagt gaaagtgatc ttgatcgaag
      421 cctcggctac agttctgccc ggcttcgatc acaagacgat cgacgaagcg gcgcaatcgc
      481 ttgcatgctt aggcgtcgag gtacgcacgg gcacgatcgt cgagcgcgtg gaagccaatc
      541 gggtgatcgt gaaaccgatc ggatttgcgg tgcttgaaga aatcgaaacc gaaacattga
      601 tctggacagg cggcatccgc gcaaattcac tcatcgctca ttcaggtttg aagattggcg
      661 agcgcggcgc ggcgatcgtc gatgaatact tgcacgcgat cgatcaacat gaagtctatg
      721 tgctcggcga cagcgcagtg gtgcgcgatc cgcgcgatgg acatatcgct acaccgtgtg
      781 gtcaactggc cgtgaagcaa ggccaatatg cggcgaaaga tattttggcg gatgtgaagg
      841 gcgatatcca tcgaccttac attccataca tggatggtct gttgatttcg ctgggttcct
      901 atgcgggcgt cggcacgatc gggccggtgt gggtgcgaag attgatcgcg cggctggcaa
      961 agatcggcgc ggagacacgt tacttgtaca atgtcggcgg ggtgcgattg gtgtcatcgc
     1021 gttggctggg cttgcgacat gagtgggtgg cgttgaatcg tgggctgaga aatttattgg
     1081 gacaccgtag atcgttaaga gcgaatcaca ccgcggttaa gtgaatgagt ccacccgcag
     1141 gtttccgcct ctgcgggcgg gaatggaaat ttgagatgcc ttcattaaaa aatatttggg
     1201 tgaatccaaa aacaaagatc attagcgatg acggagagac atcagtcatt gaagtcagcg
     1261 gcatgatctg tgaatggggt tgagcgcgaa atgtgacagc ggctctggca gagctgccta
     1321 acgtcgaagc gatcgagcac gcggccggaa ccgatcagtt cacgatcaaa catcagggcg
     1381 agctcagcag cgcggctgta gcagtggatc gaattgtgat ttttaagtgg ttgagaaaat
     1441 tcctgaatag gctgaggggc tatgaacaag gaaggtaaag aatcaacatg ataaaacgaa
     1501 tcgttattcc cggtggcagc gggttcctcg gacaagcgct gattgatcat tgggcagccc
     1561 gcgattacga aattgttgtg ctgacccgct cttcaacggc aacaaacagc caggcaagat
     1621 ttattcagtg ggacggcaag acactcggtg attggagtgc gtggctagaa ggtgctgagg
     1681 ccatcatcaa cttgacgggc aagagcgtca attgtcgtta cacgcctaaa aaccgcaacg
     1741 agattatcgc ttcgcgggtc aattcggtaa atgcaattgg tcaagcgatt cagcgctgca
     1801 cacaacctcc taacgtttgg atacaagcca gttcacttgc tatttacggt gacgcgggcg
     1861 atcgaatttg cgacgaatct gcgccttacg gccaaggctt tccagttgaa acatgcatcg
     1921 tgtgggaaca agcattagcc gcactatcct tggcacatat ccgcaccgtc gcgcttcgga
     1981 tcggtttcgc gctcaatcga tccgggggcg cattcgggcc attaaagaat ctggccaagt
     2041 tctttatggg tggcacagca ggaagtggca aacaatatgt gagctggatc catatcaacg
     2101 atctgaatag aatgtttgat tggtgtcttg aacgagacga tttgtcaggc gtgtttaatg
     2161 caaccagccc gcgaccgatg accaacgccg aatttatgcg agcaattcgc cacgtcctgc
     2221 atcgaccgtg gagcccgcct attccaagtt gggcagtaca attcggctct atcatcttaa
     2281 gaaccgaggc cgaactcgtt ctgaggggac ggcgctgcat ccccaaacgg tttagcgatc
     2341 ttgatttcaa gtttgaattt cctgaattga ttccggcact gcaaaatctt actgcctgaa
     2401 ttcaattcta atgcacggag gtgtgagatg tcgatcgata tgcagacact tttaatcgtt
     2461 ggcttggcgg cttacatcct gggcatgttg acggcgctgc gcatggagca tggcaaaagt
     2521 cattaaggtg tgtgttaccg gatgctaaat aaaaaaggca tccgcataat cagggcggcg
     2581 gctcagcggg gcgcgcttag gccgccgccc atcaattctg aaaattcaaa ccgcgaagat
     2641 gctaagggtt tataatgcga ggaagaacta tgcccgatat tgatgaacaa attcaaaaag
     2701 cgcagcaagc cggtgagttc gatcgactgc gcggcaaggg caaacccttt acgcacctgg
     2761 acagcgatcc cttgcggcat ttattggatg cgcagggttt cacgccgcac tggctggaag
     2821 ccgatcacga gattcagcgc aagatcgaat tggccgagca agcgatcaag cgcacctacg
     2881 aatgggtgat ggagctttgg agcagcggca gcgccgatcg gcatttcgcg cgagaagagt
     2941 ggcggcgcgc gctgaaaatc ttcgatgagc
//
LOCUS       DASRTJ010000769         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_226843,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000769 DASRTJ010000000
VERSION     DASRTJ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226843"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1376)
                     /locus_tag="VFF70_15490"
     CDS             complement(36..1376)
                     /locus_tag="VFF70_15490"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46141.1"
                     /translation="MKTRILLILIVVFSLIPLRSAAAQGEHLVLAFYYAWFSPSSFGS
                     TKTSDQPITPYTSSDRSTIERQVAEARQAGIDAFVQSWYGPDGGVNNQTESNFSTLLD
                     VALANNFKAAVDFETSGPFFKNTTDVKNALTSLLSSHALHPAYLSVSGKPVIFFWQNN
                     RYTVETWTDIRDAVDPYHNSIWIAEGTNLDYLRVFDGLHLYTIAWSADPRATNVTWGQ
                     RVREKAIELGSFKYFVGTAMAGWNDKLLHRSGSFARDRANGDYFRSSFSGVTQSNADW
                     AIITSFNEWPEGTQIEPSATYGDDYLNLAAELAEVYRTTASSYLIAPTETPIPTNTPT
                     VTPTPTFTPTPTKTPTPTPTNTATPTTTPTATPTPTPTSTDTPTPTRTPVKISQQLPN
                     AALAAGGQSLLPTPIPRNSSQSDNSLTGYIIGGLFMFLAAIIVGWSLGRRAKAR"
     gene            1564..2118
                     /locus_tag="VFF70_15495"
     CDS             1564..2118
                     /locus_tag="VFF70_15495"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZY46142.1"
                     /translation="MTLIEIFWTFMKVALFTWGGGPASLPIMQREVVDHGWVTQEEFA
                     DAVALGNALPGPIAPQMGVYIGYRVAGVGGAIAGMLGSVVPTSILMLILVAAFVQSSK
                     LEWVNSILAAVRPAVIGMLLWTAYDIGAKVLDKNNQGIGAALQNNWDKFLLVAAAFIA
                     LTFFKVHPAIVVVIALVFGLIAYR"
     gene            2162..2632
                     /locus_tag="VFF70_15500"
     CDS             2162..2632
                     /locus_tag="VFF70_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZY46143.1"
                     /translation="MRDTIQQDPNCFICRKHRGEISIPGGAIYQDDLVYAGHAQIREG
                     QITTYLGYLMVEPKRHADTLADLTDEEAQALGLLITRLSRALIATENAERVYLFVINE
                     LVHHVHFHVVARYPSAPREYWGPRVDEWQDAPHGGPQEIAALCRRIQNHLSSKQ"
     gene            2629..>2917
                     /locus_tag="VFF70_15505"
     CDS             2629..>2917
                     /locus_tag="VFF70_15505"
                     /EC_number="3.5.4.43"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transformation of hydroxyatrazine to
                     N-isopropylammelide and ethylamine in the atrazine
                     degradation pathway; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZY46144.1"
                     /translation="MTTLLIRNIRTLITLNADRQPIQNGSILVRDNKIEFVGASDDPQ
                     LDRFPKPDRIINGDRLIVMPGMVNTHHHLYQTLTRCIAIDSGLFNWLKTLYP"
BASE COUNT          675 a          728 c          821 g          693 t
ORIGIN      
        1 tgatttactg atggtggacg cagagctaag tagaattatc gagctttggc gcggcggccg
       61 agtgaccaac ccacgataat cgccgcgagg aacatgaaca atccgccgat aatatagcca
      121 gttaacgaat tatcactctg cgacgaatta cgcgggattg gcgtgggcag aagcgattga
      181 ccaccggccg ctaaggcagc attcggtagt tgttgagaaa tcttcaccgg tgtgcgggtt
      241 ggtgtcggtg tatcagtgga tgtcggtgtg ggcgttggtg tagcggttgg tgtcgtcgtg
      301 ggtgtagcgg tattcgtcgg cgtgggcgtc ggcgttttcg tcggcgtcgg tgtaaatgtt
      361 ggtgtaggtg ttacggttgg cgtgttcgtc gggatcggag tttcagtcgg ggcaatcaaa
      421 taactgctgg ccgtcgtgcg atacacctcg gccaactctg ccgcgagatt gagataatca
      481 tcgccatacg tcgcgctcgg ttcgatctgc gtgccttccg gccattcatt aaaagaagta
      541 atgatcgccc aatcggcatt cgattgcgtc acgccggaaa acgacgatcg aaagtaatcg
      601 ccattcgctc gatcgcgcgc gaacgagccc gaacgatgca gcagcttatc gttccaaccg
      661 gccatcgccg tgccgacaaa atatttgaag gagcctaact cgatcgcttt ttcgcgcacc
      721 cgctgacccc acgtcacatt cgttgcgcgc ggatcggccg accaggcgat ggtgtataag
      781 tgcagtccgt cgaagacgcg cagataatca agattggtgc cttcagcaat ccagatcgag
      841 ttgtgatagg gatcgactgc gtcgcggatg tccgtccaag tttcaacagt gtagcgatta
      901 ttctgccaga aaaagataac gggcttgccg ctgacgctga gatacgccgg atgaagtgca
      961 tgcgaactga gtaatgaggt caacgcattt tttacatcgg tggtgttctt gaaaaatggg
     1021 ccgctcgttt caaaatcgac tgcggctttg aagttgttgg cgagcgctac atcgagcagc
     1081 gtgctgaagt tcgattcggt ttgattgttg acgccgccat ccgggccata ccacgattga
     1141 acaaacgcat cgatcccggc ctggcgcgct tcggccacct gccgctcgat cgttgatcga
     1201 tcgctcgacg tgtaaggcgt gatcggttga tcgctggtct tggtcgaccc gaacgagctg
     1261 ggcgaaaacc acgcgtaata aaaggctaag actaaatgtt cgccctgcgc ggcagccgat
     1321 cgaagaggaa tcaaactgaa gacaacgata agaattaaca gtattctggt tttcatcgag
     1381 gcgcgattgt accagaggaa tgtgcatcga gcaatggcta ttgagtcatg tggtcgattt
     1441 gtcgtggagt tgggtagtag gatagtgagt tggtgacgag tgaaacgtga tacgtgatgc
     1501 gtaagcggct gtccgatacc cgctacgcag tacgtgatca acgaaaggat caagatgacc
     1561 atcatgacac tcatcgaaat tttctggaca ttcatgaaag tcgcattgtt cacgtgggga
     1621 ggcggcccag cctcgctacc gatcatgcag cgcgaagtcg tcgatcacgg ctgggtcacg
     1681 caagaggagt ttgccgatgc ggtcgcgtta ggcaacgccc tgcccggccc gatcgcgccg
     1741 cagatgggtg tgtatatcgg ctatcgagtt gccggagttg gcggagcgat cgctggaatg
     1801 ttgggcagcg ttgtgccaac gagtatcttg atgttgatcc tcgtcgcggc cttcgtgcaa
     1861 tccagcaaac tcgaatgggt taattcgatt ttggccgcgg ttcgaccagc cgtgatcggc
     1921 atgttgctgt ggacggcgta tgacatcggc gctaaggtac tggacaaaaa caatcaaggc
     1981 atcggcgcgg ccttacaaaa caactgggac aaattcttgc tcgtggctgc ggccttcatt
     2041 gcgctgacgt tcttcaaagt ccatccggcc atcgtcgtcg tgatcgcact cgtgtttggg
     2101 ttgattgcgt atagataact ggttgcttgc actcttgagg taatctatac tttcatgaag
     2161 tatgcgtgac acaattcagc aagaccccaa ctgtttcatc tgccgcaagc atcgtggcga
     2221 aatatcgatc cccggcggcg cgatctatca agacgatctg gtctacgccg gacacgctca
     2281 aattcgtgaa gggcaaatta cgacttatct cggttacttg atggtcgagc ctaaacgcca
     2341 cgccgataca ctagccgatc taaccgacga agaagcgcag gcactcggcc tactgattac
     2401 gcgtttgagt cgcgcattga tagccaccga gaacgccgag cgtgtttatc tattcgtcat
     2461 caatgaattg gtgcaccatg ttcactttca cgttgtggca cgttatccca gcgcgccacg
     2521 cgaatattgg gggccgcgtg ttgatgaatg gcaggatgcg ccgcatggcg gtccacagga
     2581 aatcgcagca ttgtgtcgaa gaatccaaaa tcatttatcg agcaaacaat gactacactt
     2641 ctcattcgca acatccgcac cttgatcacg ctgaatgctg atcgtcaacc aattcagaat
     2701 ggatcgatcc tcgtgcgcga caacaagatc gaatttgtcg gcgcatcaga tgatccgcaa
     2761 ctcgatcgat ttcccaagcc cgatcggatc atcaacggcg atcggctgat cgtcatgccg
     2821 ggcatggtga atacgcatca tcatctctat caaactttaa cgcgctgcat agcgatcgat
     2881 tccggtttat tcaactggct aaagacctta tatccaa
//
LOCUS       DASRTJ010000770         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_183331,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000770 DASRTJ010000000
VERSION     DASRTJ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183331"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..801)
                     /locus_tag="VFF70_15510"
     CDS             complement(169..801)
                     /locus_tag="VFF70_15510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZY46145.1"
                     /translation="MKFYDVSLTISNDLPTWPGDPSVSLQRVSDMDKGDGANVSRLDC
                     GVHTGTHVDAPVHFVPGGSGVDTLSLDTLIGMCEVRRVPDEAKAISAEILEALQIPFG
                     TIRVLFRTSNSAIWARGENKFQTNFVAIDQSGAEWIVAHGIKLVGVDYLSVAPFDAGL
                     PTHDILLRAGVIPIEGLNLSQIEPGEYLLYCLPLKLKDADGAPARVVLIH"
     gene            complement(851..>1960)
                     /locus_tag="VFF70_15515"
     CDS             complement(851..>1960)
                     /locus_tag="VFF70_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZY46146.1"
                     /translation="RTGCLVRSSYLPARFMWLARTQPKLLQEVNRWISIGEYLFDQFF
                     DRSLVSTSIASWSGLLNRHTLKWDSEWFKWLPVSIEQFSSLTDASEPISGLKSEWASR
                     WPMLKDVPWFPAIGDGAAANLGSGCSTSSQVALTLGTSGAMRVALSGTPEKIPFGLWC
                     YRIDRTTSLLGGATNEGGNVYAWLRQTLLIDTDDKFDAAINALEPNAHGLTALPFIAG
                     ERSPGYAGDVRVSITGLSINTQPIEIARAMLEAVAYRFAIIYQRIESAHPPGGFESSG
                     RIIASGGGLLRRPAWLQIFADVLNRSVIASSEKEATSRGAALIALRSIGAIKSFDEVP
                     ADLGQTFESHPDRHAIYLKAIERQKELYDLLITRH"
BASE COUNT          442 a          555 c          513 g          450 t
ORIGIN      
        1 gcccgatcgg cgaggtatgg cccccacttc ttccaatcga tctcgcgcgt ggtggtggca
       61 ttcaggcgtt gatgttcggc ggttgtggat tgcggcatga tgttcccccg taaggcgtga
      121 tgtgttttac gaaatacgcg ctacgcatta cttgttacgc atcacctgtt agtgtattag
      181 caccactcgt gctggcgctc catccgcatc tttcaacttc aacggtaaac aatacagcag
      241 atattccccc ggctcgatct gcgacaggtt caaaccctcg atcggaatca cgccggcgcg
      301 cagcagaata tcgtgcgtcg gtaagcctgc atcgaacggc gcgactgaca ggtaatccac
      361 accgactaat ttgatgccat gcgcgacaat ccattccgcg ccggattgat cgatcgcgac
      421 gaagttggtc tgaaacttat tttcaccgcg cgcccagatc gcagagttgc tggtacgaaa
      481 caatacgcga attgtgccga atgggatttg cagcgcttcc agaatttctg cgctgatcgc
      541 tttggcctca tccgggacgc ggcgcacttc gcacatgccg atgagtgtat cgaggctgag
      601 cgtatccacg ccgctgccgc ccggcacgaa atgcaccggc gcatcgacat gcgtgccggt
      661 gtgcacgccg cagtctaagc gagaaacatt cgcgccgtcg cccttgtcca tatcggacac
      721 gcgctgcagg ctgactgagg gatcgcccgg ccacgtaggc aagtcatttg agatggtgag
      781 tgagacgtcg tagaatttca taaatgattc tccattgatt attggcgacg cactcattac
      841 tattcactaa ttagtgacga gtaatgagca agtcgtatag ttccttttgc cgctctattg
      901 cttttaagta tatcgcatgt cgatccggat gcgactcgaa ggtctggcca aggtcagccg
      961 gaacttcgtc gaatgatttg attgcaccga tcgatcgcaa cgcgatcagt gccgcaccgc
     1021 ggctcgtcgc ttctttttcg gatgaggcga tcaccgatcg attcagcacg tccgcaaaaa
     1081 tttgcagcca cgccggacga cgcaataatc cgccgccgct ggcgatgatc ctcccggagc
     1141 tttcaaaacc accaggtgga tgcgcagatt caattcgttg ataaatgatc gcgaagcgat
     1201 aagcgacggc ttccaacatc gcgcgcgcaa tctcgatcgg ttgggtgttg atcgacaggc
     1261 ctgtgataga cacacgcaca tcacccgcat aacccggact gcgctcacct gcgatgaagg
     1321 gcagggcggt cagtccgtgc gcattcggtt cgagtgcatt gatcgccgca tcgaatttgt
     1381 cgtcggtatc gatcaacaac gtttggcgca gccaggcgta aacattcccg ccttcatttg
     1441 ttgcgccgcc gagcagtgac gtagttcgat cgatccgata acaccacaag ccgaacggaa
     1501 ttttttcagg cgtgccgctg agcgccacgc gcattgcgcc gcttgtgccg agtgttaatg
     1561 caacttgact tgaagtacta cagccactac ccaaatttgc cgccgctccg tcgccgatcg
     1621 ccggaaacca cggcacatct ttcaacatcg gccaacgtga agcccattcg gattttaatc
     1681 cgctgatagg ttcgctagca tctgttaatg aagagaattg ctcgatcgag acgggcagcc
     1741 acttgaacca ttcgctgtcc catttcagag tatgtcgatt gagcaagccg ctccatgacg
     1801 cgatcgaagt gctgaccaac gatcgatcaa agaactgatc gaataaatat tccccgatcg
     1861 aaatccagcg gtttacttct tgaaggagtt ttggttgcgt acgcgccaac cacatgaagc
     1921 gcgccgggag ataactcgat cgcaccaagc agccggttcg
//
LOCUS       DASRTJ010000771         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_185756,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000771 DASRTJ010000000
VERSION     DASRTJ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185756"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..295)
                     /locus_tag="VFF70_15520"
     tRNA            complement(224..295)
                     /locus_tag="VFF70_15520"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Gln,seq:ctg)
     gene            complement(367..867)
                     /locus_tag="VFF70_15525"
     CDS             complement(367..867)
                     /locus_tag="VFF70_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZY46147.1"
                     /translation="MYSLRSYDLTQAADVAANQRGEISPRQRLAFDEVARVANRPALI
                     MIAVTAILFALPIMLLWQTPGSIGDKLSATVVVIAFVAAIIIVPFLRERAHSIKHRQE
                     ILNGQIEQAAGEVRWQFTHAIARTTDRQLQSIYGPLTLPPGNYRFYYLPKSGGLLSAE
                     KIGQPQ"
     gene            1028..2347
                     /locus_tag="VFF70_15530"
     CDS             1028..2347
                     /locus_tag="VFF70_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZY46148.1"
                     /translation="MEPRNTELSDREIEILQLLANGLSNKEIAAKLFLSVNTIKVHLR
                     NVFAKIDVQSRTEATLYAIQHGHVTVVVDQLSGAIEESPSVPTILIEPPLPIGKRATL
                     IGLVAVSIGLALLIGPKSIAQSGSQAQPFSDSALTNAAFNSAASETAWQALTPMTLAR
                     GRLAATSIGTQIIAIGGEANGGVTSLVEIYDSVTQRWRLGSNKPTPIANVSAAVVANK
                     IIVPGGYTVSGVPTTTVEAYDLTTDSWSRLSPMPAPRFAYSIAVYKDQIYVFGGWDGS
                     NYSNSVFVYDAMNDRWTTRTPMPIARGFSGAAVLENAIYVVGGYADGHEFDTCERYLP
                     KDDRWETCAPMTVARGGLGLVTISSRLYAVGGGWTGYLTFSERFDPQADAWTVAPTPF
                     TGQWRGMGITTLGTDIYALGGWSGQFLGVVEKYSPFPFSIFVPSVTK"
     gene            complement(2381..3064)
                     /locus_tag="VFF70_15535"
     CDS             complement(2381..3064)
                     /locus_tag="VFF70_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZY46149.1"
                     /translation="MGIKFTWYGHAVLGLEIDKHKLIVDPFLTGNPAANITPDKVQAE
                     YILISHGHSDHVGDAIDIAKRTGATIITNNEIAGWFNSKGIEKTEGQHIGGGHMYPFG
                     YLKLTQALHGSRLPDGSNGGNPCGFLITADKKKIYLACDTGLFGDMKLIGEEGIDLAV
                     LPIGDYFTMGPDDALRAAKMLQAKNIVPVHYNTFDLLKQDAGKWAKTVEKETGSKVHI
                     LKPGESFSL"
     gene            complement(3162..>3397)
                     /locus_tag="VFF70_15540"
     CDS             complement(3162..>3397)
                     /locus_tag="VFF70_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZY46150.1"
                     /translation="GAPQQDKWIARNLDRTGAIVGIGVGGAFDFIIGKQKRAPKWIQR
                     IGLEWLYRLIREPWRWKRQLALPRFVWKILTER"
BASE COUNT          796 a          864 c          923 g          814 t
ORIGIN      
        1 cgcacccttg aaaaaatccg gcgacgaagt gaggcgtgcc tcaaaaatca ccaacgcatt
       61 gtgccatgcg cccggcggta ggctgaccaa taacccgtct cgactgcctt tgatgacaat
      121 gccatcatcg atcataatag cggaatctca ttgtgcggga ttatagcaca tcctctatgg
      181 ttttgataaa aaaccttgcg aagatttatt cgcaaggttt aggctggggg tgagggattc
      241 gaaccccccc atacagattc agagtctgct gtcctgccag ctagacgaac ccccaagatc
      301 gaacgcggct attctatcat gcgcggataa gttccgcaag ggcgaccgga ttttttgagc
      361 acagaatcac tgcggttgac cgattttctc ggcggatagc aacccgccag atttcggcag
      421 atagtagaat cggtaatttc ccggcggcaa ggtcaacggc ccatagatcg attgcaactg
      481 ccgatcggtt gtccgtgcga tggcgtgagt gaactgccaa cgcacttcac ctgcggcttg
      541 ttcaatctgc ccgttcagta tttcctgccg atgtttgatc gagtgagcgc gctcgcggag
      601 aaacggtacg atgatgatcg ctgcgacgaa cgcaatgacg acgacggtgg cactgagctt
      661 atcgccgatt gagccgggtg tttgccacag cagcatgatc ggcagcgcaa ataaaatcgc
      721 ggtgacggcg atcataatca gcgccggtcg attggcaacg cgggccactt catcaaaggc
      781 tagccgctga cgaggtgaaa tctcgccgcg ttgattagca gctacatcgg cagcttgagt
      841 gagatcgtaa ctgcgcagtg aatacatatg ctcattgtaa tcggtaatgc gtgaaagatg
      901 aagtgatgcg tgaagagtga attgtgaaaa gtggaatgtg ataggtaacg agtaatgcgt
      961 acgggttttg cgacaagcca tcagccattt gctatcagcg atttgccatc atggtagaat
     1021 acgaggcatg gaaccacgca acaccgaact cagcgatcgc gagatcgaaa ttctgcagct
     1081 actggccaac ggcctcagca acaaagagat tgcggcgaag ttgtttctca gcgtcaatac
     1141 gatcaaggtg cacttgcgaa atgtctttgc caagatcgat gttcagagcc gcaccgaagc
     1201 aaccttgtac gctatccaac acggccatgt aacagttgta gttgatcaat tgtcgggagc
     1261 gattgaggaa tctccaagtg tgcccacgat tttgatcgag ccgccgttgc cgatcgggaa
     1321 acgggcaact ctaatcgggt tggtggcggt ctcgatcgga ttagcactgt tgatcgggcc
     1381 gaaatcgatc gcgcaaagcg gatcgcaggc gcagcctttt agcgattcag ccttgactaa
     1441 tgcagcgttc aactctgcgg caagtgagac tgcatggcag gcactgacgc cgatgacatt
     1501 agcgcggggt cgattggccg ccacatcgat cggcacacaa atcatcgcca tcggcggcga
     1561 agcgaacggg ggtgtcacta gcctggtcga aatatatgat agcgtgactc agcgttggcg
     1621 tttgggatcg aacaagccga cgcccatcgc gaatgtttcg gcagccgtag ttgctaacaa
     1681 aattattgtg cccggcggtt atactgtttc gggagtacca accacgaccg tagaagccta
     1741 tgatctgaca actgatagtt ggtcgcgctt gtcgccaatg cccgcgccgc gttttgctta
     1801 ttcgatcgcg gtttacaaag atcaaatcta cgtgtttggc ggttgggatg gatcgaatta
     1861 ttctaatagc gtgtttgtat acgacgctat gaatgatcgc tggacgactc gcactccgat
     1921 gccgattgct cggggcttta gcggtgcagc cgtgttggag aatgcgatct atgtggtcgg
     1981 cggttatgcg gatggacatg agtttgacac ttgtgagcgt tatctgccga aagatgatcg
     2041 ctgggagaca tgtgcgccga tgaccgttgc ccgtggcggc ttgggactag tcacgatcag
     2101 cagtcgatta tatgcagtcg gcggcgggtg gacgggttac ctgacgttta gcgagcggtt
     2161 cgatccgcaa gcagacgcgt ggaccgttgc gcccacccct tttacgggac aatggcgcgg
     2221 catgggcatc accacgcttg gcaccgatat ttatgcgttg ggcggctgga gcggacaatt
     2281 cttgggtgtc gtggaaaaat acagcccatt cccatttagt atttttgtgc cgtcggtgac
     2341 gaaatgagaa agggcagatg gcctcgcccc aacaatatca tcacaaacta aaactctcac
     2401 ccggcttgag gatatggacc ttcgagccgg tttctttttc aacagtcttc gcccacttgc
     2461 ctgcatcttg cttgagcaga tcgaacgtgt tgtaatgaac cggcacgata ttcttggctt
     2521 gcaacatctt cgccgcacgc agcgcatcat ccggacccat cgtgaagtaa tcgccgatcg
     2581 gcagcaccgc cagatcgatg ccctcttcgc cgatcaattt catatcgccg aacaagccgg
     2641 tatcacaggc cagatatatt ttctttttat cagccgtgat caaaaagccg cagggattgc
     2701 caccattcga tccatcgggc agcctcgagc catgcagcgc ttgggtaagt tttagataac
     2761 cgaagggata catgtgaccg ccgccgatat gctgtccctc ggtcttctcg atgcccttgg
     2821 agttgaacca accggcaatc tcattgttgg tgatgatcgt cgcgccggtg cgtttggcaa
     2881 tatcgatcgc atcaccgacg tgatcgctat ggccgtgcga gatcaaaata tattctgcct
     2941 gaaccttatc gggcgtgata tttgcggccg gattgccggt caaaaaggga tcgacgatca
     3001 atttgtgttt gtcgatctcc agccctaaca cggcatgtcc ataccacgta aatttaatgc
     3061 ccatgtttat cctccgagta gagttgagct aatgatagca tgagatgatg agtaatgggt
     3121 aacgagtaat gagtaattcc tattgcgtgc cgcttgtctc actatcgctc tgtcagaatt
     3181 ttccacacaa acctcgggag cgctaattgc ctcttccatc tccacggttc tctgatcaat
     3241 cgatacaacc attccaaacc gatgcgttga atccatttag gcgcgcgctt ctgtttgccg
     3301 atgatgaaat cgaatgcgcc gccgacgccg atgccgacga tcgctccggt gcgatcgagg
     3361 ttacgcgcga tccatttgtc ttgctgcggt gcaccat
//
LOCUS       DASRTJ010000772         4743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_186626,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000772 DASRTJ010000000
VERSION     DASRTJ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186626"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1249)
                     /locus_tag="VFF70_15545"
     CDS             complement(32..1249)
                     /locus_tag="VFF70_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZY46151.1"
                     /translation="MSTQPPSSTPPPPAPIAAGTQTYTCPQCASKMSFDAKSGLLACP
                     SCGHKMPVPSATDAVQEHDLMQALQNTVGKSTGYGTEMKSINCQNCGATTNVQPNVTS
                     TTCPFCGSNQIIEQKPDPNMIQPESVVPFAVDESRANMLFRTWLSKGFFQPGDLKQLG
                     GGQKLRGVYLPFWTFDAHAESDWWAESGTYYYETEWVTVTRDGKTVREQRQVQKTRWW
                     PSSGHHADNYDDVLVYATKSIDVRILQKVYPYDTAKLLNYKPEFLSGWAAESYQINLA
                     DGWNIGKGNINSEEYSKSDRQVPGDTHRNLRVNTSLSNLTYKHVLLPVWVASYRYNSK
                     TFQFMINGQNGKVQGDKPISWIKVAIVVVIVLAIIGAIVYFVARSKSEGSSSTGELLM
                     WQQYVMAHVNMLL"
     gene            complement(1310..4132)
                     /locus_tag="VFF70_15550"
     CDS             complement(1310..4132)
                     /locus_tag="VFF70_15550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZY46152.1"
                     /translation="MSDNHASPTTPRFFGLLVLTILVASALRLIEVGTTPPGLHYDEA
                     ADTIIAEQIARGDSAPIFVEAYTGKEVLFFNWAAAWMKLIGPSVFAMRLAAAMLGILT
                     VALTYRVVKEMLSPPLALSGRGAGGEGVALLATIFIATSFAHILMSRLGFRSIAEPFI
                     QSIALAALFRGLRLNRLKWIVIAGVFVGLNLYTYLAARLFPVAVGVLFLYMIIADRGQ
                     RRQRMIQFLVLTLCAIIIFLPLGIYFINNPAAFITRIQQVAPRADQPIALLDNIGRAL
                     GMVFVSGDPYIRFNEPYRPLFPFLLGILYSVGLIVALIGLFRSRAARRRFAYFAITSI
                     TLIMLLPTALAVNEITPSNLRAIGLLPIVFVFPALGAWWLLKKINSVISSRATARNLS
                     LTEVEIPRFARNDKLFSRLNPRSIILSILVATTIDASVTYFGQYVHEPQLYIQSDGDL
                     TDISALLNHTDTSHDQVYIAALHYRHPTAAALANDYNTFKWLTGNQIVVIPDESAYLF
                     FARLALPDQAWLDRFLPPSMLIDAPLAPDGQTNYREYHLSAQPTIKPQAEMKVNYGNI
                     IQLMGYDREAHAESGRTASVTLYWRVLNKPDRGDYSTFIQLNDQWGFEWGQNGSFDYP
                     SEEWEPGEVIINRIDVPVAAGAPPGDYELRVGLFSQSTNQRINIVANDGSFGGTIARL
                     SPIEIERASKPSSVSDLGIGINVEREITAGLKLLGYSRGAQSIHQNEKFFMTLFWQSE
                     SMLNDAAISIQLKGDSTTITLTTTLPVHGLYPFSQWLPNEIVADQYALRVPIDAPPGQ
                     YTLEVSIGAGSSIGLGTIEIVQANRIMVEPEIDHRMNVMLGDAIELVGYNLDQSHLTL
                     IWRSIKSLDQDYTVFTHVLDQSGQQIAGQDNQPLNGSYPTSRWSAGEYVIDPYAINVA
                     SGQYAIEVGLYDPETGAHLGQMIQLK"
     gene            complement(4142..>4743)
                     /locus_tag="VFF70_15555"
     CDS             complement(4142..>4743)
                     /locus_tag="VFF70_15555"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZY46153.1"
                     /translation="ADLVVHSGTKYLGGHGDVMAGVIATDARRASGLRELIKVYGSNL
                     GPFEAWILLRGLKTLALRMRQHCANALQIAYWLSDQVSISKVIYPGLSDHPQHAIAQR
                     LFRDNSFGGVVAFELRGAGEPEVFRFMEALKLIRTGTSLGDVGSLLVYPAQSSHYYLS
                     PEERHRQGISDGLVRLSVGIEDAQDIIADLQVALNMISQ"
BASE COUNT         1148 a         1334 c         1205 g         1056 t
ORIGIN      
        1 gcgattaatt ctccgatcaa agttcagaga tctacaacag catattcacg tgcgccatca
       61 catattgttg ccacatcaac aattcaccag tcgaactgct gccttccgac ttcgacctcg
      121 ccacgaagta cacaatcgcg ccgatgatcg ccagcacgat gacgaccaca atcgccacct
      181 tgatccagga aatcggcttg tcgccttgca ctttgccatt ctgtccgttg atcatgaatt
      241 ggaacgtctt gctgttgtag cgatacgagg ccacccacac tggcagcagc acatgcttgt
      301 aggtcagatt cgataaactt gtattcacgc gcaaattgcg atgcgtatcg cccggcacct
      361 gccgatcgga tttactgtac tcctcgctgt ttatattgcc cttgccgatg ttccagccat
      421 cggccaagtt aatctgatac gactcggccg cccagcccga tagaaactcc ggcttatagt
      481 tcagcagctt cgccgtatcg tacggataga ctttttgcaa aatccgcacg tcgatgctct
      541 tcgtcgcata caccagcaca tcatcgtagt tatcggcgtg atgcccgctg gacggccacc
      601 agcgcgtctt ctggacctgc cgctgttcgc gcaccgtttt gccatcgcgc gtgaccgtca
      661 cccattccgt ctcataatag tatgtgcccg actccgccca ccagtcactc tcggcgtgcg
      721 catcgaatgt ccaaaagggc agatacacgc cgcgcaattt ttgtccgccg cccagttgtt
      781 tcagatcacc gggttggaag aaacccttac tcagccaggt gcggaacagc atgttcgccc
      841 ggctctcatc gaccgcaaac ggcaccacgg actccggctg gatcatattc gggtccggct
      901 tctgctcgat gatctgattc gatccgcaga acgggcaggt cgtcgatgtc acgttcggct
      961 gcacattcgt cgttgcgccg cagttctggc agttgatcga cttcatctcc gtgccgtagc
     1021 ccgttgactt gcccacggtg ttctgcaaag cctgcattaa atcgtgttcc tgcacggcat
     1081 ccgtcgccga cggcaccggc atcttgtgtc cgcacgacgg acaggccaac aaaccggact
     1141 tggcatcgaa actcatcttg ctggcgcatt gcgggcaagt ataagtctgc gtacctgcag
     1201 cgatcggcgc aggcgggggt ggcgtacttg aaggcggctg ggtactcata tcaatcactc
     1261 cactcaaaga tggcgctaag tatatctagt ctcggacaaa atgcaaaact catttcagct
     1321 gaatcatctg acccaagtgc gcgccggttt caggatcata tagaccgacc tcgatcgcat
     1381 attgacccga cgcaacattg atcgcatatg gatcgatcac atactcgccc gccgaccaac
     1441 gcgacgtggg atacgatccg ttcagcggct gattgtcctg tccggcgatc tgctgtcccg
     1501 attgatccag cacatgcgta aaaaccgtgt aatcttgatc gagagatttg atcgatcgcc
     1561 agatcagcgt gaggtgagat tgatcgagat tgtagcccac taactcgatt gcatcaccga
     1621 gcatcacatt cattcgatga tcgatctccg gctccaccat aatcctattg gcttgcacga
     1681 tctcgatcgt gcccagcccg atcgacgatc cagcgccgat cgagacttcc agagtgtact
     1741 gaccgggcgg cgcatcgatc ggcacgcgca aggcatattg atcggccacg atctcattcg
     1801 gcagccattg actgaacgga tataatccat gcacaggcaa agtagtggtt agggtgatag
     1861 ttgtactgtc acccttcaat tgaattgaaa ttgccgcatc attcagcatc gattcactct
     1921 gccaaaagag tgtcatgaaa aacttttcat tttgatggat ggactgcgct ccacgcgagt
     1981 aacctaacaa cttcaacccg gcggttattt cacgctcgac gttgattccg atccccaagt
     2041 cactcacact cgacggcttt gaagcacgct cgatctcaat cggcgacaag cgtgcaatcg
     2101 ttccgccaaa cgatccgtca tttgccacga tattaatgcg ctgattcgta ctctgcgaaa
     2161 ataatcccac tcgcaattca taatcgcccg gcggcgcgcc tgccgcaacc ggcacatcga
     2221 tgcgattgat gatcacctca ccgggttccc attcttctga cggataatcg aacgagccgt
     2281 tctgtcccca ttcaaagccc cattgatcat ttagttggat gaatgtcgag taatcgccgc
     2341 gatcgggttt gttcaatacg cgccaataga gtgtcacact cgccgtcctg cctgattcgg
     2401 cgtgtgcttc tcgatcgtag cccatcaact gtatgatatt cccataattc accttcattt
     2461 ccgcttgcgg cttgattgtt ggttgtgccg acaaatgata ttcgcgatag ttcgtctgcc
     2521 cgtccggcgc cagcggcgcg tcgatcaaca ttgatggcgg caagaatcga tcgagccacg
     2581 cttgatcggg cagagctaat cgagcgaaaa ataagtatgc tgattcgtcg ggaatcacaa
     2641 cgatctggtt gccggtcagc cacttgaaag tattgtagtc attcgccaag gccgctgcgg
     2701 tcggatgccg ataatgcaat gcggcaatat agacttgatc gtgtgacgtg tcggtatgat
     2761 tcaatagtgc gctgatgtcg gttaagtcgc catcgctttg aatgtatagc tgcggctcat
     2821 gcacatattg cccaaagtac gtcacgctcg catcgatggt ggttgcaacc aaaatcgaaa
     2881 gaattattga acgtggattg aggcgagaaa ataatttgtc attccgagcg aagcgaggaa
     2941 tctcaacttc cgttaagctg agatttctcg ccgttgcacg gctcgaaatg acagagttta
     3001 ttttcttcag cagccaccac gcgcccaacg ccggaaacac aaacacgatc ggcagcagcc
     3061 cgatcgcgcg gaggttgctg ggcgtgattt cattgaccgc cagcgccgtc ggcaacagca
     3121 tgatcaatgt gatgcttgtg atggcaaagt aagcgaatcg acggcgagcc gctcgagagc
     3181 ggaataaacc aatcaaggca acgatcaaac ccacgctgta gagaataccc aatagaaatg
     3241 gaaacaacgg tcgatagggt tcgttaaagc gaatgtatgg atcgccgcta acgaaaacca
     3301 tgccgagcgc gcgtccgatg ttatcgagca gcgcgatcgg ttgatcggcg cgcggcgcaa
     3361 cctgctgaat gcgtgtaatg aaagccgccg gattattaat gaagtaaatg cctagcggca
     3421 agaagatgat gatagcgcag agcgtaagca ctaagaattg gatcatgcgc tgccggcgct
     3481 ggcctcgatc agcaatgatc atatacagga agagcactcc aaccgccacg gggaacaatc
     3541 gggcggcgag atacgtgtaa agattcaagc cgacgaacac acccgcgatc acgatccatt
     3601 tcaatcggtt taagcgcagg ccacgaaaca aggccgccaa tgcgatcgat tggatgaacg
     3661 gctcggcgat cgatcgaaag cccaatcggc tcatcaaaat gtgcgcgaaa gaggtggcga
     3721 tgaagatggt tgcgagcaga gcaacacctt cacccccggc ccctctccct gaaagggcga
     3781 ggggagggct aagcatctct tttacaacac gatacgttag tgcaacagtg agtatgccga
     3841 gcatcgcggc ggcgaggcgc atggcgaaca ccgatgggcc gatcaacttc atccaggcag
     3901 ccgcccaatt aaagaacagg acttctttgc cggtataggc ttcgacgaag atcggcgcgc
     3961 tgtcgccgcg tgcgatctgt tcggcaatga ttgtgtcggc ggcttcgtcg taatgcaagc
     4021 cgggcggcgt tgtgccgact tcgatcaatc gcaacgcgga ggcaacaaga attgtgagga
     4081 caagtaaccc gaagaaccgg ggcgtagtgg gtgacgcgtg attatcactc atcactcatc
     4141 actattgact aatcatgttg agcgcaactt gtagatcagc aataatatct tgcgcatctt
     4201 caatgccgac cgacaagcgt accagcccat cgctgatgcc ctgtcgatgg cgctcttcgg
     4261 gcgacagata gtaatggctc gattgcgcag gataaacgag caaactgccc acatcgccga
     4321 ggctggttcc ggtacggatc aatttcaacg cttccataaa acgaaaaact tctggctcac
     4381 ccgcaccacg caactcaaat gcgacgacgc cgccgaatga gttgtctcta aacaatctct
     4441 gggcgatcgc atgttgagga tgatcggaca agccgggata aatcactttg gagatcgaaa
     4501 cttgatcgga caaccaatac gcgatctgca gcgcgttcgc gcaatgctgc ctcatgcgca
     4561 gcgccagcgt ttttaagccg cgcaataaaa tccacgcttc gaacggaccg aggttcgagc
     4621 catagacttt gatcaactca cgcagtccgc tcgcgcggcg cgcatcggtg gcgatgacgc
     4681 cggccatcac atcgccgtgc ccgcccaaat attttgtgcc tgaatgcacg acgagatccg
     4741 cgc
//
LOCUS       DASRTJ010000773         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineae bacterium isolate SMAG_U6913 k141_188748,
            whole genome shotgun sequence.
ACCESSION   DASRTJ010000773 DASRTJ010000000
VERSION     DASRTJ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970151
            Sequence Read Archive: SRR12756251
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineae bacterium (soil metagenome)
  ORGANISM  Anaerolineae bacterium
            Bacteria; Chloroflexota; Anaerolineae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:45:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,113
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Anaerolineae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188748"
                     /isolate="SMAG_U6913"
                     /isolation_source="soil"
                     /db_xref="taxon:2052143"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="68.38 N 149.43 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFF70_15560"
     CDS             complement(<1..864)
                     /locus_tag="VFF70_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZY46154.1"
                     /translation="MKVLLVGVGGVGEAIAVVAQNRAWLEHMVLADYNLDRAREVQSR
                     LTDKTKFPIEKIDASDRAQIVALAKKYRVDLIMNAVDPIFNEEIFDAAYEAGAHYMDM
                     AMTLSSPHPTDPFHQPGVKLGDYQFARAKDWEAKKLLALVGMGVEPGMADVFARYAEK
                     HLFDEIDEIGIRDGANLEVRGYEFAPNFSIWTTIEECLNPPAIWEKDKGWFTTEPFSE
                     AEVFDFPEGIGPVECVNVEHEEVLLVPRWIKCKRVTFKYGLGEKFIGILKTIKMLGLD
                     KKEKINVKGVQV"
     gene            complement(966..1511)
                     /gene="hpt"
                     /locus_tag="VFF70_15565"
     CDS             complement(966..1511)
                     /gene="hpt"
                     /locus_tag="VFF70_15565"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZY46155.1"
                     /translation="MDSAIERILIDEQSIAAKVKELAAQISAAYQGGDELIMVCILNG
                     AFRFTADLVAHMTIPYNIDFMSLSSYPSASTKATGEVRVLLDVKNSLHDKHVLIVEDI
                     VDTGHTLNYLYQMLNTKHPASLKSCALVSKTEMRAIDVHPEWIGFHVPNEWLVGYGLD
                     WHNQYRMLPYIGVLKPEVYSH"
BASE COUNT          389 a          490 c          384 g          426 t
ORIGIN      
        1 aacctgcaca cccttgacgt tgatcttttc ttttttgtcc aagcctagca tcttgatcgt
       61 tttcaaaatg ccgatgaact tttcgcccaa cccatatttg aaagtgacgc gcttgcattt
      121 gatccaacgc ggcacgagca aaacttcttc atgctccaca ttgacgcact cgaccggccc
      181 aatgccttcc ggaaaatcaa acacttcggc ttcagagaac ggctcggtcg tgaaccagcc
      241 tttgtccttc tcccaaatcg cgggcggatt caaacactct tcgatcgttg tccagatcga
      301 aaagttcggc gcgaattcat aaccacgcac ttccagattc gcgccgtcac ggattccaat
      361 ttcatcgatc tcgtcgaaga gatgtttctc ggcatagcgc gcaaacacat cggccatgcc
      421 cggctccacg cccatgccga ctaatgcgag aagtttcttc gcttcccaat ctttcgcccg
      481 cgcgaattga taatcgccca acttcacgcc cggctgatga aacggatcgg tcggatgcgg
      541 cgaggatagc gtcattgcca tatccatgta atgcgcgccc gcttcatacg ccgcatcgaa
      601 gatctcctcg ttgaagatcg gatcgacggc gttcataatc agatcgacac gatacttctt
      661 ggccagtgcc acgatttgcg ctcgatcgct ggcatcgatc ttctcgatcg ggaatttggt
      721 tttgtctgtc aatctcgatt ggacttcccg cgcccgatcg agattgtaat cggccagcac
      781 catgtgttcc agccacgctc gattttgggc aacgacggcg atcgcctcgc caaccccacc
      841 cacacctaca agtaagactt tcatgctact ccttaaaaat tttagaccgc agaccaaaga
      901 ccgcaaacca cggctgatcg ctcttcaaca gagatcaact gcggtcggcg gtctgtcgtc
      961 tgccgtcagt gtgaatacac ttccggtttc agcacgccga tatatggcag catgcgatat
     1021 tgattgtgcc aatccaaacc gtaacccacc agccattcat tcggcacatg gaaacctatc
     1081 cactcgggat gcacatcgat cgcgcgcatt tcggtcttgc tcaccagcgc gcacgatttc
     1141 aatgaagcag gatgtttggt gttgagcatc tggtacaaat aattcagcgt gtgacccgta
     1201 tcgacgatgt cttcgacgat cagcacgtgt ttgtcgtgca ggctgttctt cacatccaat
     1261 agcacccgca cctcgcccgt ggccttggtc gaggctgacg gataactgct caacgacatg
     1321 aagtcgatgt tatagggtat ggtcatgtgc gcaaccagat cggcggtgaa gcggaacgcg
     1381 ccgttaagaa tgcagaccat gatcaattca tccccgcctt gataggctgc cgaaatttgc
     1441 gccgccagct ctttgacttt ggctgcaatc gattgttcgt caatcaggat gcgttcaatg
     1501 gctgaatcca taaacagctc ctcgctaaag ttttagtcgc ccatgatttt aagcgcattc
     1561 acagtaatgc acaactcttg tgtgtggtgc gtattgcgta ttacgtactt acctgatgtg
     1621 caactttctg aatccgtcaa gccagaaccg tcattgcgag gcgcgtcttt ttgcgccgaa
     1681 acaatctcc
//
